Text

NPDES Permit No. TN0026450 - Discharge Monitoring Report for June 2018
	ML18198A129
Person / Time
Site:	Sequoyah
Issue date:	07/13/2018
From:	Anthony Williams; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Div of Water Pollution Control
References
	NPDES Permit No. TN0026450
	Download: ML18198A129 (102)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 July 13, 2018 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway, #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR June 2018 Enclosed is the June 2018 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period. The final report for toxicity sampling conducted at outfall 101, from May 13 - May 18, 2018, is enclosed. No adverse effects were observed. The corrected May discharge monitoring report for outfall 101 T is also enclosed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fi ment for knowing violations. t ony ~- illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures): U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

PERMITIEE NAME/ADDRESS {Include Facility NameA.ocation if Different) Name _ _!YA - SEQUOYA!:!._NUCLEAR PLANT __ _ _ Address P.O. BOX2000 ------------ - __..J!NTEROFFICE OPS-5N-SQN) _ ______ _ ___ SODDY - DAISYJ.N 37384 -------- Fard!J!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMIL TON COUNTY _________ _ ATTN:Millicent Garland NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 G PERMIT NUMBER DISCHARGE NUMBER From 18 06 01 To 18 06 30 MAJOR (SUBR 01) F - FINAL DIFFUSER DISCHARGE EFFLUENT

- - NO DISCHARGE D Form Approved.

0MB No. 2040-0004 I t 0~rG p~~t ~ YEAR I MO D I MO I PAY NOTE: Read instructions before complelinA this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 39.9 0 30 / 30 RCORDR 04 CENTIGRADE MEASUREMENT 00010 1 0 PERMIT Req. Mon. DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE 28.0 0 30 / 30 MODELO 04 CENTIGRADE MEASUREMENT 00010 z 0 PERMIT 30.5 DEG.C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2.6 0 30 / 30 CALCTD 04 UPSTRM DEG.C MEASUREMENT 00016 1 s PERMIT 3.0 DEG.C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILYMX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1863 0 30 / 30 RCORDR 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 0 30 / 30 CALCTD TREATMENT PLANT MEASUREMENT 03 03 50050 1 0 PERMIT Req. Mon. MGD MGD CONTI CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE 0.016 0.035 0 23 / 30 GRAB 19 MEASUREMENT 50060 1 0 PERMIT 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0.2 0 30 / 30 CALCTD 62 CHANGE MEASUREMENT 82234 1 0 PERMIT 2.0 DEG -- l) CONTI CALCTD ~ EFFLUENT GROSS REQUIREMENT DAILYMX C/HR NUOUS / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared uf ~my ~ident TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified ersonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the pe on or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 18 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following injections occurred: Flogard MS 6236 (max calc. was 0.033 mg/L, limit -- 0.20 mg/L), Spectrus BO 1500 (max calc. was 0.037 mg/L, limit -- 2.0 mg/L). EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ 3YA - SEQUOYA~NUCLEAR PLANT __ _ _ Address P.O. B0X2000 --- - -------- __ _(!NTEROFFICE OPS-5N-SQNl _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F - FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY-DAISY...!.N 37384 -------- PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 FacJ!i!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMIL TON COUNTY _ ___ _ ____ _ EFFLUENT ATTN:Millicent Garland MO 06 ¥ 0o~iS 81 NG _p;-~-~-E -M,,,_ o _ D"""" Ax_ r 01 1 To [ 18 r 06 30

- - NO DISCHARGE D

~-~--~--~ NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE Monitoring CERIODAPHNIA MEASUREMENT Not Required TRP3B 1 0 PERMIT 42.8 EFFLUENT GROSS REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR SAMPLE Monitoring PIMEPHALES MEASUREMENT Not Required TRP6C 1 0 PERMIT 42.8 EFFLUENT GROSS REQUIREMENT MIMINUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ,,/"" ~ ) / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER,,....,.,,.,..,..,_..,.,, __.,,.,,_,~-.. -~ ~d~ direction or supervision in accordance with a system designed to assure that qualified P. sonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the p son or persons who manage the system, or those persons directly responsible for gatherin the Site Vice President information, the information submitted is, to the best of my knowledge and belief, tru

ccurate, and complete. I am aware that there are significant penalties for submitting false informauv,,

including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was not sampled in June 2018. EPA Form 3320-1 (REV 3/99) Previous editions may be used _§Je ice res1 ent

,IGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO.

FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 18 07 11 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

i PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _2VA - SEQUOYA!:!.._NUCLEAR PLANT _ __ _ Address P.O. BOX2000 - ----- - ----- __ _J!NTEROFFICE OPS-5N-SQ!:!.l _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 103 G MAJOR (SUBR 01) F-FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY-DAISY....I.N 37384 -------- LOW VOL. WASTE TREATMENT POND FacJ.!!!y TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTONCOUNTY ---- - ----- EFFLUENT ATIN:Millicent Garland

- - NO DISCHARGE D...

NOTE: Read instructions before completinA this form. PARAMETER iX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 6.5 6.7 0 5 / 30 GRAB 12 MEASUREMENT 00400 1 0 PERMIT 6.0 9.0 SU ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE <2.5 <2.5 0 1 / 30 GRAB 19 MEASUREMENT 00530 1 0 PERMIT 30.0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE <5.0 <5.0 0 1 / 30 GRAB 19 MEASUREMENT 00556 1 0 PERMIT 15.0 20.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.022 1.114 0 4 / 30 INSTAN 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD ONCE/ INSTAN EFFLUENT GROSS REQUIREMENT MOAVG DAILYMX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT "\\ / / NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER ' '"~ ""'" ""'"""',_.. '"",~ ""' "' *-~~ -*..... ~ L TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualifie ersonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the erson or ~ice ~sident persons who manage the system, or those persons directly responsible for gather" g the 423 843-7001 18 07 11 information, the information submitted is, to the best of my knowledge and belief true, accurate, Site Vice President and complete. I am aware that there are significant penalties for submitting false i o~ion--- SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _..!_VA - SEQUOYA~NUCLEAR PLANT ___ _ Address P.O. B0X2000 ------------ __ _.{!NTEROFFICE OPS-5N-S~l-NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 G MAJOR (SUBR 01) F - FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY - DAISYJN 37384 -------- PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER FaclfilY TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMIL TON COUNTY _________ _ ATIN:Millicent Garland PARAMETER QUANTITY OR LOADING AVERAGE MAXIMUM TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE, WATER DEG. SAMPLE CENTIGRADE MEASUREMENT 00010 z 0 PERMIT INSTREAM MONITORING REQUIREMENT TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. EFFLUENT GROSS VALUE REQUIREMENT DAILYMX CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 50060 1 0 PERMIT EFFLUENT GROSS VALUE REQUIREMENT TEMPERATURE - C, RATE OF SAMPLE CHANGE MEASUREMENT 82234 1 0 PERMIT 2 EFFLUENT GROSS VALUE REQUIREMENT DAILYMX SAMPLE MEASUREMENT PERMIT MO 06 UNITS 03 MGD 04 DEGC MINIMUM EFFLUENT

- - NO DISCHARGE I xx I ***

NOTE: Read instructions before completin;i this form. QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS 04 REPORT DEGC CONTIN CALCTD DAILY MX uous 04 30.5 DEGC CONTIN CALCTD DAILY MX uous 04 5 DEGC CONTIN CALCTD DAILYMX uous CONTIN RCORDR uous 19 0.1 0.1 MG/L Five per CALCTD MO AVG DAILYMX Week CONTIN CALCTD uous REQUIREMENT / ) / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER direction or supervision in accordance with a system designed to assure that qualified rsonnel ' " """' ""'""',~ '""'"a=-... "' """'m'""-""':f Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the rson or persons who manage the system, or those persons directly responsible for gather g the information, the information submitted is, to the best of my knowledge and belief, rue, accur ~ Site Vice President and complete. I am aware that there are significant penalties for submitting false in n, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used / ~esident-- SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 423 843-7001 18 07 11 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name __ TVA-SEQUOYA~NUCLEAR PLANT---- Address P.O. BOX2000 - ----------- __ _QNTEROFFICE OPS-5N-SQ!fl _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 110 T MAJOR (SUBR 01) F - FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY - DAISYJ.N 37384 -------- Faq!!!y TVA - SEQUOYAH NUCLEAR PLANT Location HAMIL TON COUNTY - PERMIT NUMBER DISCHARGE NUMBER From ~ 18 I 06 : 01 To [ 18 - 06 I 30 RECYCLED COOLING WATER EFFLUENT

- - NO DISCHARGE I xx I ***

ATTN:Millicent Garland YEAR MO o MO PAX NOTE: Read instructions before completinQ this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT 42.8 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM IC25 STATRE 7DAY CHR SAMPLE PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT 42.8 EFFLUENT GROSS VALUE REQUIREMENT MINIMUM SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT / v- ) REQUIREMENT / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 'C"~ ""'" '"""'"'~*"-' "°'"~""'-, -**~-1~* m, ~ direction or supervision in accordance with a system designed to assure that quali

d personnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of e person or persons who manage the system, or those persons directly responsible for gat ring the information, the information submitted is, to the best of my knowledge and beli true,~

Site Vice President and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used ~_, - Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT NO. FREQUENCY SAMPLE EX OF TYPE UNITS ANALYSIS 23 PERCENT SEMI COMPOS ANNUAL 23 PERCENT SEMI COMPOS ANNUAL TELEPHONE DATE 423 843-7001 18 07 11 I AREA I NUMBER YEAR MO DAY CODE Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) Name _ _!YA - SEQUOYA!:!__NUCLEAR PLANT ___ _ Address P.O. BOX2000 ------------ __...1!.NTEROFFICE OPS-5N-SQ!il _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 118 G MAJOR (SUBR 01) F - FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY - DAISY....!.N 37384 -------- PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER FacJ!!!y TVA - SEQUOYAH NUCLEAR PLANT Location HAMIL TON COUNTY _________ _ EFFLUENT ATIN:Millicent Garland MO 06 ¥0~J981NG i,...,., P£~ ....... ~ ¥_...MO......._D"""'"AY._ I 01 ] To l~1a~I_o_s~_3_0~

- - NO DISCHARGE I xx I ***

NOTE: Read instructions before completinQ this form. PARAMETER IX QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE 19 MEASUREMENT 00300 1 0 PERMIT 2 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 PERMIT 100 MG/L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILYMX WEEK SOLIDS, SETILEABLE SAMPLE 25 MEASUREMENT 00545 1 0 PERMIT 1 MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT /~ /~ REQUIREMENT / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER """"""' -*"',_.., ~. *~* '"" --* -* ~ ~ n t TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qu,tied personnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage the system, or those persons directly responsible for thering the 423 843-7001 18 07 11 Site Vice President information, the information submitted is, to the best of my knowledge and lief, true, and complete. I am aware that there are significant penalties for submitting fa

ation, SIGNATURE OF PRINCIPAL EXECUTIVE I

including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITIEE NAME/ADDRESS (Include Facility Name/1..ocation if Different) Name _.!._VA

SEQUOYA~ NUCLEAR PLANT ___ _

Address P.O. B0X2000 ------------ __ _.(!NTEROFFICE OPS-5N-SQtil _______ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 101 T MAJOR (SUBR 01) F - FINAL Form Approved. 0MB No. 2040-0004 ___ SODDY - DAISY,_I.N 37384 -------- PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac:J!!!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMIL TON COUNTY _________ _ EFFLUENT ATIN:Millicent Garland MO 05 NO DISCHARGE D NOTE: Read instructions before completinQ this form. PARAMETER !x QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100.0 0 3 / 180 COMPOS MEASUREMENT 23 CERIODAPHNIA TRP3B 1 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE >100.0 0 3 / 180 COMPOS MEASUREMENT 23 PIMEPHALES TRP6C 1 0 PERMIT 42.8 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ~ ./ / / NAME/TITLE PRINCIPAL EXECUTIVE OFFICER, '"~ """'"""" *,~..,,;,'°'"mom,oo" '"'~-* - ~ ~ -- TELEPHONE DATE direction or supervision in accordance with a system designed to assure U,at qualifie rsonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of u, arson or persons who manage the system, or those persons directly responsible for gal ing the ~ Site Vice President information, the information submitted is, to the best of my knowledge and ,et. true, accurate 423 843-7001 18 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting alse informatio SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled May 14 - 18, 2018. The final report will be included with the June DMR. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

TENNESSEE VALLEY AUTHORITY TOXI JTY TEST REPORT Report Date: June O l, 2018

l. Facility I Discharger:

equoyah Nuclear Plant / TV

2. County/ tate: Hamilton / Tenn s e

3. NPDES Pennit #: TN0026-l50

4. Typ of Facility: Nu !ear-Fueled Electri Generalin!! Plant

5. Design Fl w (MOD): 1.579

7. 1Ql0:.).49l

8. OutfallTested: 101

9. Dates ampled: Mav 13 - 18.2018

10. Average Flow on Day ampled MOD: 1805. 1810. 1788 l l. Pertinent ite Conditions: Pr duction / operation data will be pro i<l"'d up

12. Test Oat s: Mav 15 - 22. 2018

14. Test Species: Fathead Minnows (Pimephales µtome/as)

Daphnids ( eriodaphnia d11bia)

15. Concentration Tested %):

Pimephale promelas: UV treated utfall l O l: l 0. 7. 21.4. 42.8. 85.6. l 00 UV treated Intake: l 00 C*riodaphniadubicr: Non-treat d utfall 101: I0.7._l.4.42.8. 5.6.100 Non-treated Intake: LOO

16. Permit Limit Endpoint (%):

utfall 101 : [ 2s = 42.8%

17. Test Re uhs:

utfall l O l : Pimephal s promela. : 1 2:- > l 00% eriodaplmia dubia: IC2s > I 00% Page 1 of94

18. Facilit

Contact:

Millicent Garland Phone#: (423) 843-6714

19.

onsulting / Testin0 Lab: En ironm ntal Testin2. olutions. Inc.

20. ab nla t: Jim umner Ph ne #: (828) 350-9 64 Exp sure to sampl s collected May 1"' - 18. 2018 from utfall IO I re ulted in not xic effect to fathead mianow or daphnids. The resulting J 25 alues for both species. were > I 00 percent. E posure of minnows and daphnids to intake ample re ulted in no ignificant difference fr m tJ1e controls during thi ~ stud p riod.

Page 2 of94

METHOD UMMARY Samples: I. amp ling P int: utfall IO 1. Intak

2.

ample Type: omposite

3.

ample Infonnatjon: Date Dat Date (MM-DD-VY) (MM-DD-VY) Initial (MM-00-YY ample Time ET) T) TRC* Time (ET lD ollected Lat Us dB 101 05-13-18 0755 t 05-14-18 1305 __ 9 <0.10 05-15-18 11 05 05-14-18 0655 05-16-1 1008 lntak 05-] 3-1 0812 t 05-i4-I I 05 2.2 <0. 10 Or-l;<:i-1 1105 05-14-18 0711 05-16-18 1008 LOI 05-15-18 0722 to 05-16-18 1240 1.0 <0.10 0 18 1013 05-16-1 0622 05-18-18 1037 Intake 05-15-18 0740to 05-16-18 1240 J._ <O. L 05-17-18 I 1 05-16-18 0 40 0 18 l0"7 05-17-18 0657 to 05-19-18 10 7 101 os-1 s-18 o

s 1 0 18 13 I -

1.), l.3t <0.10 05-20-18 05-21-18 05-17-18 0711 to 05-19-1 Intak 05-1 8-18 0611 05-18-18 1"'15 0.5 <0. 10 1 05-21-1

TR = Total Residual Chlorine tsamples v ere collected in two 2.S gallon *ubitainers. Temperature was measured in each cubitain r upon arTival.

4.

amp! Marupulation:. ample from utfall I 01 and intake,. ere warmed to test temperatur (25.0 +/- 1.0° ) in a warm water bath. Y-treated tlu*ough a terilizer (manufactured bv mp ror Aquatics. lnc.) for 2 minute. Page 3 of94

I. ourc

2.

gc: est Method umrna1y:

1. Te t onditions:

2. Te t Durali n:

Pimephales pr me/as In-house Cultures < 24-hours old tatic. Renewal ontrol / Dilution at r: M derately Hard ynthetic

4.

umber Replicat : 4

5.

rgani ms p r eplicat : lQ

6.

est Initiation: (Date/Tim): 05-15-18 1024 *T

7. Te t Tennination: Oat Time): 05-22-18 0926 ET

8.

e t emperature: utfatl 1 Ol : Mean= 24.6uc (24.2 - 25. l )

9.

I 0. Slati tics: Page 4 of94 eriodaphnia dub;r, Jn-hou Cultures < 24-hours old lQ 05 18 I 105 ET 05-22-18 1025 T ynthetic

ee Appendi, fi r Bench heet

1.

Results of a PimephaLe promel 1s Chronic/ 7-day Toxicit Te t. Genus specie ) (Type / Duration) Conducted May I - - 22.2018 u ing effluent rom utfall IO I. Te t Percent urv1vmg Solutions (lime interval used - days) (% Effluent) I 2 3 4 5 6 7

Control, I

100 100 100 10 100 JOO UY-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 JOO 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 iOO JOO 98 98

Control, 100 I 0 1

100 100 10 Non-treated Te t Solutions M a11 Dry Weight (mo) (% EfOu nt) (replicate number) 1 2 3 4 Mean

Control, 0.688 0.614 0.65

0.7... 0.680 UV-u*eatcd 10.7% 0.741 0.759 0.728 0.743 0.743 21.4% 0.728 0.743 0.719 0.750 0.735 42.8% 0.724 0.753 0.675 0.715 0.717 85.6% 0.729 0.756 0.630 0.705 0.705 100.0% 0.705 0.690 0.731 0.694 0.705 Intake 0.628 0.568 0.608 0.677 0.620 ontroL 0.736 0.759

0. 44 0.602

0. 85 Non-treated fC2s Value: > I 00%

Calculated U Estimat : < 1.0 TUc* Permit Limit: 2.8% Permit Limit: 2.3 TUc 95% Confidence Limit : Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = l 00/ IC2*

Page 5 of94

TOXI fTY TEST RESULT (see Appendix C for Bench h ets) Re ult fa Ceriodaphnia dubia Clu-onic/ 7-day Toxicity Te t. (Genu pecies) (T pe / Dura[j n onducted May 15 - 22. 201 using effluent fr m Outfall l 01. Percent urv1 mg Test (time interval used - days) I ulions l 2 4 5 6 7 (% Ef1luent) ontrol 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 JOO 100 85.6% 100 100 !Ov 100 ]00 100 100 100.0'Yo 100 100 100 100 100 100 100 est olutions Reproduction (#young/female/7 days) Data (replicate number) (%

ff1uent) l 2

3 4 5 6 7 8 9 10 Mean ntrol 28 28 27 29 33 28 27 28 29 27 28.4 1.7% 31 32 32 29 29 33 29 31 1") 31 l .).) I,.- 21.4% 34 32 30 31 31 32 30 30 34 36 32.0

42. %

32 35 32 37 34 37 34 30 .).) 31 33.5 85.6% 36 36 35 35 39 33 31 34 .,5 .,5 34.9 100.0% 37 33 37 39 36 39 36 34 34 37 36.2 I 1s Value: > 100% Calculated TU Estimate : < 1.0 TUc P rmit Limit: 42.8% Pennit Limit: 2.3 TUc 95% onlidence Limits: Upper Limit: _ Lower Limit: NA Ua = 1 0/L so: TU = lOO/ J s Page 6 of94

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day T xici!y Tes!. (Genus species (T pe / Duration Conducted Mav 15 - 2 __ 2018 using water from Intake Percent urviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent.) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Te. t olulions Repr duclion #y ung/lemalc/7 days) (% Effluent) Data r licate numberl 2 4 5 7 9 JO Mean Control 30 29 26 30 0 29 31 27 30 2 29.0 lntake 5 33 34 34 36 ., ') 34 35 33.9 ..) .)_ ..)..) !Cr Value: > 100% alculated T Estimate : < l.O Uc* Permit Limit: N/A Permit Limit: N/ 95% Confidence Limit ~: Upper Limit: NA Lower Limit: A and D Duration 25 Pimephales promelas May08 - l5. 2018 ors 7 day Ceriodaphnia dubia Ma 08 - l, 201 0911 7-day Page 7 of94

~ CQ Cl) Oo 0 -.. co ~ for UV-11c.11ctl P1111cphc1l~.f prr,111c1c1s J1xl Nun-,r~;olcd < r11odopl1111a,/11h1t1 5cquo1ah Nuclcm l'i:11n (SQN), 1-fTiucnl OUll:lll lO I,mJ 1111.11.!' pcr~,m11.:d Mnv 15-22. 2018 I e~1 Sample 10 Tcmpcr:11111*c <"C) Dissolved 0 X}l!CII (mt:/LJ pll (~.ti.) Conduc11111cc \\lk"linlty llurdnch ' Totul llcsidu:11 lnitinl ( OM l lfll, 2*1 7 N1111

l ft'.tl tU 2-HI
24 8 rf)nuol.

24.7 \\ l\\',,,u1frtl 24 <,

24 l!

1(1.7% 2'1.8 24 7 - 24 S ~ 24 ll 2lA% t 147

24 9 1-1.8

-H.8'Ya ~ ~ 2n 24 9 85.6% 24.9 c:.. 24 8 - 24.9 IOU% N9 248 25 0

lninlu, 25 0 24 9 - 25 I l

11 11111.

24 7

\\ ~n.. 1n.*.,lr1I 14 6 - 248 I0.7%

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~

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24 '}

~ 24.9 85.6% 1:: (i 247

250 IOU%

24.9 248 - 250 lrllu kc 24.9 24 7 - 15.0 pc Ovcrnll lc 111pc 1111nrc (°C'l /'11111:p/111/,*.r promela.1 I...,.,,,d,111(111111 d11h,n Fln:11 24 S 24 3 - 24 6 24.4 24 J. N 6 24.5 24 J - 24 6 2-1.5 24 2. 24 7 2.J.-1 2U

24 5 2,1 3 24.2 -

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25. I 24 CJ

. 2..i2 24.9 24 8

2.5 2 25.0 248. 250 24 9 24 8
25 I 24 9 24 8 - 25 2 14 9 24 7 - 25 I 24 8 24 8 -

25.0 vc ra~e 24 <, 24,, lnitlnl fi'in:il lniti:11 7 8 7.J 7 95 7 6 - l!O uJ - !i *.1 7.85 - 801 79 75 7 98 7 6 - 8 I 6~ - M ll 7 91 - ~ l~l 79 7 5 8 ()(J 77

8. I 70 -

7'J 193 - ~ l/5 so 7.j 8 01 77. RI 6 9 - ~ l/ 1 <J~ - MO<, 80 74 8.01 7 7

8 I 6 () -

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8 0-!

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8.J 6\\J 81 7 8')
8 ().4 7 8 7 9 7 9S 76
80 77.

8.l) 7X5

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8 07 7.9 7 CJ 8 00 7 7 -

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II.I 7.8

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806 80 7.9 7 911 78. IU 78 * ~! 7 88 - 811-1 8.0 8.0 7.97 H-8.2 78 - 8 I 71)1 tOJ ~ ~l inimu111 1\\ l :t~im11m 24.1 25 I 24 f, '.!5 2 Final (~unhos/cm) (mg/L CnCOJ) (mg/L CaCOJ) ('.blo1inc (mg/L) 7 68 307 (,1 83 7-1()

7.81 Jli(J
315 (10 6 1 82 -

~4 7 74 309 61 !13 7 5'l 785 1'}8

318 59 63 82 84 7 (>8

!93 149

7 84 286
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7~5 2(,7
283 7 71 242 756 - 784 231
246 7 62 17!1 7 45
7 76 171 187 7.61 154

i8 63

< 0. 10 7 1 7J 146 161 54 62 00 6-1 < U 10 - <O 10 7.60 154 56 GO < 0 10 731 779 143

lbl 56 56 6<).

60 <U 10 - < O 10 8.00 J07 61 !I] 792 - 8 ()..I JtKI

JIS 60 61 82 84 8.00 287 792
805 1811
294 8.00 173 792 - lW3 265 282 7 99 24 1 7 'll
8 OJ 237
25(1 7.97

.11<i 78Q - 802 171 17<> 7 95 Ii I 58 <,-1 < 0 10 7 81!

M 00 138 -

156 58 "8 (iO (,lj < ll 10 - < 0 JO 7 97 154 57 61 < O 10 7 88 - S (),I 147 15') 56 58 (,{) 64 <0.10 * < O iU

UMMARY / CON L SIONS Expo urest amplescolle led Ma 13 - t8 201 fr m utfall 101 r sulled in no t xic effe ts to fathead minnows or daplmid. The re ulting [ 25 alu s. tor both species. ¥ere> I 00 percent. Exposure of minn ws and daphnids t intak sample re ulted inn significant differ nc from the conlrols during thi study period.

ppcndix A DDITIO A TO f ITY TE T INFORM TIO I. Pimephales prome/as e t were conducted a cording to EPA-82l -R-02-01., October 20 2 using fi ur replicate, ea h containing ten te t organisms. p r treatm nl. Test vess ls consisted of 500-mL pla tic di po abl up. each containing 250-mL of test solution.

2.

eriodaplmia dubia Tests wer conducted according to EPJ\\-821-R-02-013 October _002 using ten repljcat ach containing 011 test organism, per treatment. e t ves els onsi ted of 30-mL p lypr pylen cup. each containing 15-mL of test solution.

1. Pim ?pha/es promela ample used in th fathead minn w test were exposed to UV light for two minut s p1ior to introduction of te ' t organi ms. UV treatment is used to ontrol interference of ti h pathogen. Thi treatment meth d was approved n November 23 _Ql5 by the State of T 1messee in a !en r fr m J sica !urphy to Terr h ek. enior Manager f TV Water P rmit.

ompliance. and Monitoring.

2.

eriodaplmia dubia N n J. Pimephale promela ne

2.

eriodaphnia dubia one Page 10 of94

1. Reagents, Titrants. Buffers, et.: All chemical were certified products used b for expirati n date (where applicable).

2. In trument : All identification, service. and calibration infonnation pertaining to laborator in trument is recorded in calibration and main enan logbooks.

3. Temperatur was mea ured by M 2550 B-2010.

4. Di solved xygen wa measured by SM 4500-0 G-20 I l.

5. The pH was mea ured by SM 4 00-H+ B-201 I.

6. Conductance wa measured by M -510 B-2011.

7. Alkalinit was mea ured b M 2 20 B-2011.

8. Total hardnes wa m asured by M 2"'40 C-2 11.

0 Total residual chlorine wa measured by RI N 7-7 -1 77. E Toxicit Te l M th d : AJJ phase of the stud including, but not limited to, sample collection, handling and storage, gla ware pr paration, test organism culturing/acquisition and acclimation, te *t r~ani *m ha:1dJing during te L, and maintaining appropriate te t conditi ns were conducted ac ording t the pr t c l as described in this report and EPA-S21 -R-02-0] 3. 1 known d<;:viations,,; ere noted duri11g the tudy and ar rep rted herein. REFEREN ndix D for ontrol chart infonnati n I. Test T pe: 7-da, clu* nic te ts with results *pre d as I r valu in g/L K I or a 1.

2.

tandard T icant: Potassium hlorid (K I cry talline) for Pim '}Jhales promelas. dium Chlorid Na I ry tallin for Ceriodaphnia dubi t.

3. Dilution Water Us d: Moderat ly hard syntheti water.

4.

lalisti s: To ale oft via.re Version -.0 wa u ed for tatisticaJ anal e. Page 11 of 94

I. PDE Permit o. TN0026450. U PA.

3.

tandard Methods for the Examination of Water and Wast water. 2_nd Edition. 2012.

4.

peratine.. ProcedlU"e, En ironmental T sting Page 12 of94

Page 13 of94 equ ah Nuclear Plant Biomonitorino May 15 - 22. 20 I 8 pp ndix B Diffu r Dischar 0 e oncentrations of Total Re idual Chi rm, Diffuser Discharg n ntration f Ch mi als ed t Contr l Mi robiologicall lnduced CotT sion and M llu k During To. icity Te t ampling

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO 02/06/2005 <0.0042 0.028 0.010 02/07/2005 <0.0116 0.028 0.010 0.007 02/08/2005 <0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005 <0.0042 0.028 0.010 02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 06/06/2005 0.0043 06/07/2005 0.0103 06/08/2005 0.0295 a 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 07/19/2005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 0.014 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 O.Q18 0.014 10/30/2005 0.0068 10/31/2005 0.0112 11/01/2005 0.0104 11/02/2005 0.0104 11/03/2005 0.0117 11/04/2005 0.0165 11/14/2005 0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 0.0231 11/18/2005 0.0200 11/19/2005 0.0116 Page 14 of94 H-lSOM mg/L Quat 0.037 0.037 0.037 0.036 0.036 0.036 0.035 0.036 0.036 0.035

Date 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 0.0084 0.017 0.0103 0.0164 0.017 0.0305 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 0.0260 0.0151 0.017 0.0172 0.0154 0.018 0.0086 Page 15 of 94 H-ISOM MSW mg/L 101 Quat mg/L Phosphate 0.037 0.037 0.037 0.037 0.015 0.036 o.oi5 0.036 o.oi5 0.036 0.015 0.036 o.oi5 o.oi5 0.041 0.041 0.030 0.041 0.030 0.041 0.030

Date 02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09 05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09 11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09 05/09/10 05/10/1 0 05/11/10 05/12/10 05/13/10 05/14/10 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L TRC Phosph EO/PO Quat ate 0.0197 0.017 0.0237 0.017 0.0104 0.021 0.0155 0.017 0.0106 0.017 0.0129 0.0415 0.0053 0.0049 <0.0141 <0.0160 0.025 0.0152 0.0255 0.0306 0.0204 0.0093 0.0192 0.0055 0.0100 0.039 0.0171 0.039 0.0041 0.039 0.0099 0.039 Page 16 of94 H-150M MSW mg/L 101" Quat mg/L Phosphate 0.0446 0.0396 0.0396 0.0397

Date 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 05/01/2011 05/02/2011 05/03/2011 05/04/2011 05/05/2011 05/06/2011 11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 11/11/2011 05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012 08/12/2012 08/13/2012 08/14/2012 08/15/2012 08/16/2012 08/17/2012 05/12/2013 05/13/2013 05/14/2013 05/15/2013 05/16/2013 05/17/2013 09/15/2013 09/16/2013 09/17/2013 09/18/2013 09/19/2013 09/20/2013 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M Hypo-mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L chlorite TRC mg/L Copoly-DMAD Azole Quat mg/L mg/L Quat mg/L Phos-mer EOIPO Quat TRC J>hate 0.0122 0.0112 0.0163 0.0107 0.0132 0.04 0.04 0.0155 0.04 0.0179 0.04 0.0089 0.0168 0.0225 0.0141 0.0239 0.0242 0.0231 0.041 0.0145 0.041 0.0298 0.041 0.0174 0.0256 0.028 0.037 0.0209 0.037 0.0279 0.028 0.0076 0.0446 0.0099 0.0091 0.039 0.0096 0.039 0.0229 0.0063 0.0072 0.0379 0.0107 0.036 0.0379 0.0217 0.036 0.0379 0.0172 0.0173 Page 17 of 94 MSW Floguard 101 MS6236 mg/L mg/L Phos-Phosphate ohate 0.029 0.029 0.029 0.029 0.029 0.032 0.064 0.064 0.064 0.032 0.032 0.03 0.03 0.03 0.03 0.03 0.03

Date 05/04/2014 05/05/2014 05/06/2014 05/07/2014 05/08/2014 05/09/2014 09/07/2014 09/08/2014 09/09/2014 09/1 0/2014 09/11/2014 09/12/2014 08/09/2015 08/10/2015 08/1 1/2015 08/12/2015 08/13/2015 08/14/2015 10/18/2015 10/19/2015 10/20/2015 10/21/2015 10/22/2015 10/23/2015 05/15/2016 05/16/2016 05/17/2016 05/18/2016 05/19/2016 05/20/2016 07/31/2016 08/01/2016 08/02/2016 08/03/2016 08/04/2016 08/05/2016 04/30/2017 05/01/2017 05/02/2017 05/03/2017 05/04/2017 05/05/2017 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M Hypo-mg/L 222 mg/L mg/L -PFmg/L mg/L 73551 CT1300 mg/L chlorite TRC mg/L Copoly-DMAD

Azole Quat mg/L mg/L Quat mg/L Phos-mer EO/PO Quat TRC obate 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 0.04 0.04 0.04 0.0070 0.0074 0.0195 0.03 0.0275 0.03 0.0213 0.0192 0.0182 0.0162 0.0125 0.0120 0.0130 O.o174 0.0156 0.0209 0.0210 0.0361 0.0254 0.0261 0.0091 0.03 0.0093 0.03 0.0209 0.03 0.0298 0.0218 0.0260 Page 18 of94 MSW Floguard 101 MS6236 mg/L mg/L Phos-Phosphate obate O.o3 0.03 0.03

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Date Towerbrom Floguard Spectrus BD1500 Nalco Spectrus mg/L MS 6237 mg/L 73551 CT1300 TRC mg/L Quat mg/L mg/L Phosphate/Zinc EO/PO Quat 07/23/2017 07/24/2017 0.0124 0.04/0.01 07/25/2017 0.0081 0.04/0.01 07/26/2017 0.0232 0.04/0.01 0.03 07/27/2017 0.0179 07/28/2017 0.0296 0.03 5/13/2018 5/14/2018 5/15/2018 0.0229 0.030 5/16/2018 0.0159 0.030 5/17/2018 0.0133 0.030 5/18/2018 Page 19 of94 Floguard MS6236 mg/L Phosphate 0.0275 0.0275 0.0275

Page 20 of94 quo ah uclear Plant Biomonitoring Ma 15 - 22, 2018 Ap endix C barn of Custody Records and Toxicity st Bench She ts

BIOMONITORING HAIN OF CU TOD\\' RE ORD Pag 1 of 1 Cl~ t: TVA En ironmental Testing Solution In. Delivered By ircle One* ~ 351 Depot Street. Fed x UP Bus Client Pr'1Ject Name: equoyah NP Toxicity h:l, - Asheville C So("\\~(!_ 'De I t"t,.... ru P.O. Numb r. NIA Other specify): I 28801 G n ral Comment : Fathity Sampled: Sequoyah NP 11,,X,.5o.,..p(-eS tro~ IOI S~\\-.,(2 t>'lf/1 t>n 5*t~,/9' PD umber: TN0026450 Ph ne: 28-350-9364 7NA' 5a... p>>!. tro- ~-\\. ~pier@ /)825 r,... S'*P/-/? Colli 1d By*~ .PA-/\\IT6fl,1 ~s ~ -~ Fa 28-350-9368 ' IOI o,ac~ ~le. wo.1,~t~ ~ ~ec ~ Lr.I<-//~ :.7"_,., ~ t V1'$S /l'fd4!.! l,,J<:rc Oal/, o-./t!.c/ + ~pf p,i s, i!~ Field I dei1'til cati n /..._ rcfrnb~ ~lleC!i n Date/Time ontoiner low PM'~ \\333i ample D criptlon Number& (MOD) Rain vent? Volume (Mark as Appr priale Laboratory Use Collecced V lfYcs. No T ETSl~g Arrival Temp B Time Appear* In hes umbtr ancc tart End ~ .-A...11 -101-TOX Comp ~-13 -t F S'-1'1-1? .Z(-. gal) X leo51"i-02.. 2 -~ -c_ 6' b '155 Er !)~55 ~,. I libS.oo tpS SQN-INT-TO C mp S *l3*1J> 5"*/1/* 1? I (2. gal) N x_ tt30S11.{.o:!) '2...1.. (_ ! \\~O~ -j. of 12. £1 {) 712. ~ '- Sample Custody Fill fn From Top Down -'J. (_\\I~~--\\ ~Al c;. \\~,. 5f\\t1.ILCS lu..l.e.\\\\J ~t) l~ 6-aob c.,r~\\;:t\\CN. ~ Relinqui hed B ignature ; I 1me Recei ed B ( ignature at 1me AL_fl~ \\~~ 5-Jt/,J? /o?S8'Ei '~ /(.. 9_~ /)_ ~i->\\(. os--J~<.;1t t;t'o,~YE"t ~-1,J~ J~ V $ow\\(_ ~f( /v----_ Q £ g,_ b J'-1 Y-1f' / L); ()1-E"'\\S os-,'4-,~ \\~OS £X \\'n~ ~, V I., In 1ru lmn

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-1 Whole Effluent Sample Receipt Log Page~

sample temperature performeo using Sample Rece,v ng Thermometer: N 160928622 Date Time Received Received Sample Project Sample Sample name and description State Comments Received Received

_by from Temp. ( 0C) number number 05-16*18 0827 K. Keenan

5. Gouge 3.1 13340 180516.01 Baxter Healthcare NC 05-16-18 0957 K. Keenan Fed - Ex 2.3 13341 180516.oi Enfield WWTP NC 05-16-18 0957 t<. Keenan Fed

Ex 2.3 13342 180516.03 Scotland Neck WWTP NC 05-16-18 1240 J. Sumner TVA Courier 1.0 13337 180516.04 TVA* Sequoyah Nuclear Plant -101 TN 05-16-18 1240 J. Sumner TVA Courier 1.2 13337 180516.OS TVA - Sequoyah Nuclear Plant - Intake TN SOP G4
E><hlbit G4.2, revision 01-03-12

BlOMONITORJNG CHAIN OF CUSTODY RECORD Page _ l_ of _ 1_ ~. CINtft: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Pr$ct Name: Sequoyah NP To icity 351 Depot Street. FedE UP Bus Client ~- P.:Number: NIA Asheville, NC Other (sp cify : """' I"\\ ;.-, I),. \\,\\;e f I co 28801 General Comments: f", g FadMity Sampled: equoyah NP too~ ~-pi-es tfo,.., lo\\ 5... ""'~l<re ()~5D 6 " ,Jg-I NPO Number: TN0026450 Phone: 828-350-9364 -r.,., K ~pl-t>S -fro-. "I:...i.. Sa.1--t~ler@ 01Jt/ t>" 5-18-tJ Collected Byd. tJ. MltJrt:... Fax: 828-3 50-93 68 _o4

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,. bl k. Biomonitoring samples are preserved b taring them at °C od hippin them in ice. The hold ti111e for each s mplc i

hours from the time of collection Therefore, pie e c Hect and hip in su h a, that the laboratory will receive the. amples with ample time 10 ini1ia1 te. ting, ithin that time frame. amples shipped 11em1ghl on Friday via Fed ~x or P. must e marked for aturda. d Ii e r th y will not arrive until Lhe following M nda.

I Date Received 05-18-18 OS-18-18 05-18-18 05*18-18 Time Received 0948 0948 1315 1315 Received Received by from J, Sumner Fed - Ex J. Sumner Fed

Ex J. Sumner TVA Courier J. Sumner TVA Courier

\\ Whole Effluent Sample Receipt log Page t..S

sample 1en1peranJre performed using Sample Receiving Thl!fmomete. SN 160928622
sample Project Sample Sample name and description Comments tate Temp. (

0C} number number 2.1 13341 180518.01 Enfield WWTP NC u 13342 180518.02 Scotland Neck WWTP NC 1,1/1.3 !3337 180518.03 TVA

Sequoyah Nuclear Plant
101 TN 0.5 13337 1aos18.o~

TVA* Sequoyah Nuclear Plant - Intake TN SOP G4

E><hibit G4.2, revision 01-03-12

I 1 1 Page Io 7 Chronic Whole Effluent oxicity Te *t ( 1 P -821-R-02-013 Method 1000. Sp de : Pimepllales promelas Project#: __ , -~-;':)_:-S_.\\ ____ _ Dilution preparation i11(ormnlio11: Dilution prep (%} 10.7 21.4 42.8 85.6 Effiuc111 volume (111L) 267.S 535 1070 2140 Diluent volume (mL) 2232.5 1965 1430 360 Total volume (mL) 2500 2500 2500 2500 Test orgllllism i11formalio11: Org,anism source: In-house culture Age: < 24-hours old Spawn date: a::,.00..-\\& Hatch dates and times: OS*\\~-\\ i ,,;. "\\..S "Cl (\\~-\\C-,, *\\& o<;OS Transfer vessel infonnation: pH=,_.,, Temperature - ,_-\\.°':) Average transfer volume: <0.25 mL 100 2500 0 2500

s. oc ounty; Hamilton Outfall: 101 Co111111e11ts:

Each conce,iu-ation was UV-treated for 2 minutes to remove pathogenic Interferences. Test in(or111ntio11: Randomizing templale: LllrH\\" 0~ Incubator number and \\~ she! f location: Anemia CHM number: CHM984 DIJ in<> i11/or111atio11 for weioht deter111i11r11io11: Date/ Time in oven: OS,l.."l..*\\k 00\\~ Initial oven temperature: l.ro ' L Date/ Time Ollt of oven: 0S*1:~*l.R (Qoo Final oven temperature: l,,()" c..... Total drying time: "1.'.\\ o..>D ~ Daily feeding mul reuewnl i11/or111atio11: D:iy Dntc I Morning feeding Aftcmoo11 r;:c<lin" Tc t initintion, Snmplc numbers u cd MllSW rcncwnl, or hntch tcrrninntion used Time Arrnlyst Time Annlyst Time Annlyst Outfall IO l Intake 0 05-15-18 hc,o )'( \\t..\\O u \\0-8 vi \\.g os.H..n_ li oS \\'-\\. 03) oswtR A I 05-16-18 ,..~,~ \\I \\1.l~ \\,( oC\\"\\..S .\\. <n. \\~ OSI~* ~ O~*ll>*lf f\\ 2 05-17-18 CJ'\\..\\1.- (JI~() vi l:>~Q \\1 ll 1&0S 11... o"" le os,1c..n<;; o~- 1l.t~ II 3 05-18-18 u* ~- en'-\\\\ \\,f \\~~~ OC\\S'c ,.,t l.'-1:61\\o- ()~ \\iU';llo. ~ l(\\ C:.*\\ Ir:,* t.f p.. 4 u "~ 05-19-18 ~'-\\.(.,. l,t I; -.\\<; l/ D0.S""\\ lRoSti-0~ If Cl c; lf-0--\\ 0~*11,,-1 i A 5 05-20-18 u* ,czos1c-o-t CrOO \\( \\'\\OO IA 1nl\\ \\A 1 1-t\\S \\8', If\\ ~-\\\\, *lfA. 6 u* I\\ 05-21 18 Ol.H.) ).\\ \\ 1..t I,) of.\\ '2.S M li O.i le, (f!) l.foS tf -O"l o~-11.*u I'\\ 7 05-22-18 \\J. oo.1-lo *~ Co11trol i11formatio11: l)\\J. c..n '-'1""tO I.... Acccprnoce criteria St1111111nry of test e11tlpoi11ts: % Mortality: () 7. s20% 7-day LCso '> 1001 Average weight per initial larvae: 0.1.,go NOEC 100 7. Average weight per surviving larvae: O.l!>fO >- 0.25nig,'larvae LOEC >1007. ChV ) 1007 ICzs ~1007. Page 27of94 SOP AT20 - Exhibit AT20.3, revision l l-01-14

~ ~ J I j Page 2 of7 Date: 05-15-18 urvivnl nud Growth Dntn Da CONTROL 10.7% 21.4% A B C D E F G H I J K L 0 10 10 10 10 10 10 10 JO 10 10 10 10 I 10 1 0 IC) l () ll) IC) ID ID 10 I l) I () ro 2 It) l t) 10 IC) lO 10 IC) IC) l~ l () l~ I D 3 It) ID ID lU IO ( t) IC) IC) ID ( \\J lO I() 4 't, lQ I () 10 \\0 10 lO 10 I () 10 ro to 5 ID,o 10 10 IC) \\b \\Q I lJ ID IQ l~ 10 6 I (.) 1-0 l \\) ID lU l C) LC) LO lO lO l D LO. 7 ID , a / t) I 0 'D ro 'D /Q /0 tO lQ LO = P:m wcigh1 (mg) Trny color code:: ~(~ /5. t.f l 1a.1i /5,5.2. Ho.13 15.~0 /{o, 1~ 15.St \\3.04 l'i. IC\\ /fl.OJ.. Jt~5 15 14 nnlyst: TS Dnlc: Q~-Ql-li o-P:111 + Lnrvnc weight (mg) /\\n:tly t: i{ ,2.. l\\ t f.'lb 2.?.a; 'l.\\.16 "?.'2,.1.,l -Z.,j,'?)~ -i1..,'\\ 20,<41 21.<J7 -z.~."s -z.1.~I 2:z.1..\\.I D:ilc: O'i*t3*,g_ C = L:1 rv:ie wet ht (mg)* B - A - __ os. ~ ,.*t\\,.~'\\,. 'LB.. ~,.u,...,~,. '°',.so 11:tntl c:11 olnted. ,.1.~ Anni *st: t l.,.R& (., '"" !...S~ \\Vcighl per initi:tl number oflnrvnc (111r1) = / lniti. I number of lnrvn~ ~' ~ ~ A"::,0 Cl} ~ 'V:p ~ "'q. ~~ ~ /\\\\.'>\\ Homl cnlculntcl.l. ..} A. N; nntysr: el o* o* o* o* o* o* o* o* o* o* o* o* Avcr:iuc wcigh1 per Percent rccluction O.i~ initinl number of from contr*ol (%) D.lo &b - °'.-~1. o.,oS - g. 1. 7. lnrwtc(mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, rn = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and rerurned, w = wounded. Comments: Page 28 of94 SOP AT20 - Exhibit AT20.3revision11-01-14

I J Pag 3 of7 Species: Pimeplwles promelas Client: TVA/ Scguovah Nuclear Plant, Outfall 101, UV-treated Date: 05-15-18 Survival anti Growth Data Day 42.8% 85.6% JOO% M N 0 p Q R s T u V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 I 10 IO co >o IO IQ C 0 ID ID 10 ID 10 2 tD It> co IC) LO l C) l~ /Q It) l t\\ ta ID 3 lQ tC) to \\0 l 0 l () I D tD 1G IQ 10 l() 4 to 10 IC) l C) I~ I() I~ IC) 10 10 10 I~ 5 I() IO IC) I t) tD l t) \\ t) \\D LC) l C) ID ({) 6 IO 10 10 10 10 ( () ( 0 tQ IQ co lD 10 7 l I:) l\\) 'O I l) ID (Q ID l () 10 ID 'G 'C) A = Pan weight (mg) Tr.

color code::

ao.,, IY.Co4 l"l,4 \\ /J*I. ~9 15.15 jLI, 30 15. 'i1 15..39 J'i. 9J 15.15 15..t<o 14. '15 111.n Aunlysr: 'T5 Dnrc: t1.l"i -/\\ I -18 U = Pnn + Lnrvnc weight (mg) Annlyst: ~ '1.1.<<c -i.,.<:\\~ L,\\.'2.-l U-'!>o 21.S"\\ 1.~-~ "U.ti\\ -z.1.'il 11.lO 2i..fb '2..7..'2.b 'Z.1.1_3 Ont,,: as. 1~-1&- -C = Larvae weight (mg)"' U-A llnn!l calculnrc!l. j ,.1..~\\,.s~ i.,.,,;,. \\ 'S,.1..~,. '=,\\o t.-.;>Q,.os,.oS l....'\\O,.2>\\ lo.'\\~ Analyst: Weight per initial number or lnrvnc {mg) - C / lniti:il number orlnrv:ic ~ w J::,

t,

°" ,;;a '.!:><:) "6' Q(., r>> ~ ~ llnnd cnlcul~tc!l. ~ 'fl 'O \\9 Annlysl: o* 'J> o* 0 o* 0 o* o* o* 0 o* o* C:>' Avcrngc weight ll(r Pcrceul reduction -3.~7. initi:il numbcr or rrom control (%) o.-, Y1 -s.c:; 1. c.,os - ~. & 7. o.,os larvae {mid Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm - unusually small, lg= unusually large d&r = decanted and returned w = wounded. Comments: Page 29 of94 SOP A TIO - Exhibit A T20.3 rcvi ion 11-1-14

l 1 I ' *----~ Page 4 of7 pccics: Pimeplwles pl'omelas Client: TVA/ Seguovah Nuclear Plant, Outfall 101. UV-treated Date: 05-15-18 Survival and Growth Data Day 100% lntake Control - Non-treated y z AA BB cc DD EE FF GG HH 0 10 10 10 (0 (0 (0 10 10 I 10 ( () 10 10 ,o lO IC) 10 2 {O ll) *o 10 l \\) co ( t> 10 3 / t) It) 10 10 \\D co IC),o 4 I a 't) 10 I~ (0 I (:) (0 ( i:) 5 IO t 0 ID 10 l C) ID l(J 10 6 q,4. ro IG lb ID 10 ID /0 7 q I () ,o ID It) ( I:) IC) '('J.. ,'\\ = l':,n,vcighl (mg) Trny color code:: Annlysl: T.$ ~re..j /5. 7'o !5.5G, J5.(i1 15.39 Jta5 /5.0a. J5.o5 15.fo9 D:11 ; ()6-0\\-lg' B = P:1n + L:1r\\'nC weight (mg) -U.1.~ /\\n:1lyst: ~ 22.o\\. -u.,1.. ZZ.lb 2.1.'-\\ \\ "Z-2.../o\\ 'Z\\,'19 1..l :1\\ D11tc: CS-1..:?.*-*i C = La rv:1c weight (mg) = B - A ~.1.8 S.loi

c..08 L,.11

,.31.. -,,$'\\ l.,.t.\\-l 1..,.01-1-lnud cnlculntcd. Analy 1: t}1 Weight per initial number or lnr\\'nc (mg) = C / lnilial number orlnrvnc N' ~ J, ~ ..J} ~ ~,v' 0 Hnn!I cnlculatc!I. ~"' '9 '-a ~ ~ '1) ....g An:1lys1: t¥1. o* o* o* 0 0. c) o* 0 ,\\v mgc weigh! per Pcrcc111 rcd11ction g.17.

ivo, inilinl number or from control (%)

D* lo'1..0 0.1., %5 Nfut.AW.

lnrv:1c (me)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk "" sick, sm = unusually small, lg "" unusually large, d&r = decanted and returned, w = wounded. Comme11ts: II JJ Page 30 of94 SOP A TIO - Exhibit AT20.3, revision 11-01-14

Environmental Testing Solutions, Inc. C**f'n1!1r.tl<"l t",\\.1 Utl lt OI, '*uH1.:=-tfiJ (-'on11ol, ll\\t*1tr,11rtl Ui11f\\,. 11.,** JU% N!-~6"* wo~. IU\\\\'",.. l11t11kt ~ Ou1111r11*, M U \\ ;1 h1 t ~ 1lMSI>: 1111:tkC'j Omrnt.u' M *u,.ah*t : t*MSI>: K1'-pffnl, (:T Oil n ff I IJ C u E I C II I J I. ~I 0 I' 0 It ~ I II X \\ ' AA llR 1..i,t11 11.t.WUon.r ftNl1t1t*l'\\ l ii( l.;f" ioe l~n...,. 10 Ill 10 IO 10 IU 9 to 10 ,n H> Ill ID 10 IU 10 10 II) IU 10 Ill 10 10 Ill 10 10 10 10 IO 10 II) 10 1n II) 10 111 10 IQ Ill IU ju 10 Ill 10 10 1\\1 10 It> Ill 10 Ill IU w 10 Ill 10 In J(l 10 10 9 Otrl. 7 II.I A*ll,f,ll*tli:;111 t-.:, 14 o, i<Ol 1< Ul "(IJ ll *II ll ll ll 'l I~ 13 ll ?0 '" 7J IHI IJ l/,1 14 ' " lbU1 IJJS 15 ll 14 b-1 HJI IHq ll Jl 100 ll 87 l~ JQ UY1 D7l IS 2<, lJ ~~ 141'> IS7* 11 ~b l~t... IS JO ISO PMSD TVA I Sequoyah uclenr Pinnt, Outfall LOI Mn, 15-22, 2018 l'i111epl11tl1t.1* 11r"111clfls hronic Whole

mucnt Toxic.it Tc.1 l~l'/\\-!!21-ll.-02-013, i\\lcthotl IOO<J.0 Qunlit) Control Vrrilirntiou or D11111 Entry, nl ul:11ion ', antl tati. ticnr,\\11:11 xc~

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&w*rot1trtt11011 "fel-1lrw, SofatlOn 11\\C.. S1art'Oa1e* 5/15/2016 TV A/ equoyah Nuclear Plant, Outfall I 01 May 15-22, 2018 tatistical Analy e Larval Fish Growth and Survlv~I Tost-7 011y Growth Test 10. PpFRCR Sample ID* T\\/A I SON Ouuall 101 End Dale 5/22/2018 Lab ID ETS* nv,r. Tesung Sol Sample Type. OMR-Di charge Monitonng Report Sample Dale. May 2018 Protocol: FWCHR-EPA-821-R-02-013 Test Spec es: PP-Pimephales promclas Commenls UV-treated Cone.OJ. 1 2 3 4 \\JV-Control 0.6890 0.6140 0.9530 0 7630 Non-Control 0.7360 0.7590 0.6440 0 6020 10.7 0.7410 0 7590 0.7280 0 7430 21.4 0.7280 0 7430 0.7190 0.7500 42.8 0.7240 0.7530 0.6750 D 7150 85 6 0.7290 0 7560 0.6300 0.7050 100 0 7050 0 6900 07310 0.6940 lnla 0.6280 0,5680 06080 0.6770 Transform; Untunsrormod Cone,% M11an N-Moan Mo.an Min Max Cli'/, N I.Sr.it uv.c nlrol 0,6795 0 916 0.6795 0.6140 0 7630 9 322 4 Non-Con trot 068.53 1 0000 06853 06020 0 7590 10.871 4 10.7 0,7428 1 083ll 07428 07280 07590 1 71 1 4 -2.351 21.4 0.7 50 1 ons 07350 0.71 0 0 7500 1 914 4

2 063 42 0 0 7168 1 0460 0 7 16 0.6750 0 7530 4 494 4

- 1,385 85.6 0 7050 1.0288 0 7050 06300 07560 7 663 4

0.948 100 0 7050 1 0288 0 7050 0.6900 07310 2 618 4

-0.948 In kc 0.6203 0 905 06203 0.56B<l 0 8770 7 306 4 Auxllla!l'. TttSts Statistic Shaptro-Wllk*s Te.sl lnd,calas normal dis11'lbu1ion (P > O 0,1 0.9.SS *1 Bartlett's Tesl lnd,cales equal variances (p = O 05) ,, 2468 The control means e no1 sign,ficantl)!'. di!feren1 <e ~ 0. 91 l 0.11761 H;i:eothosls Tost (1-tall, 0.051 NOEC J..OEC ChV TU MSDu MSOe Dunnett's" I 100 >100 0 06483 0.09541 neatmenls vs UV-Control l.ln*~r lr,tol'polat,on (200 Resamplo$) Point ,cos 1c 10 IC15 IC20 IC25 IC40 IC50 > 100 > 100 > 100 >100 >100 >100 >100 so 95'Yo CL(Exe) Skow C> *.. C 0 a. Iii c:: 0 09 08 0 7 06 05 0.4 03 0 2 0, 00 -0 I -0 2 0 1-Tailod Crltic~t MSO 2 410 0 0648 2 410 0 0648 2, 410 0.0648 2 41 0 0.0648 2 4,0 0.0648 Critical Q 1Jij4 15 0863 2 44691 MSB MSE 0 0021 0 00145 50 100 Doso 'II, 0 8 i 06 e os ~ 04 0 ,._ 03 0 2 0 1 Ooso*Rosponso Plot o+--~--~--~--~--~--~---j ~ i Page 32 of94 1-lall, 0.05 lcvel al significance l&otonlc Moan N*Mo'"n 07191 I 0000 0 7191 1.0000 0 7191 1.0000 0 7168 0.9968 0 7050 0.9804 0.7050 0.9804 Skew Kurt -0.0045 1 4844 ! F-f',ob di 0 25399 5, 18 ,so

,wltonm*nUI 1u1ln9 S0lu\\J0n1. Inc. Start Date. 5/1 5/2018 TV / equo ah uclear Plant Outfall 101 - Intake Mc y 15-22 2018 tati ti al Analyses Larval Fish Growth and Survival Test-7 Day Growth Test ID: PpFRCR Sample ID TVA/ SQN lnta e End Date 5/22/2018 Lab ID. ETS-Envir Testing Sol Sampl Type. DMR-Discharge Monitoring Report Sample Date: May 2018 Protocol. FWCHR-EPA-821-R-02-013 T st Speci s. PP-Pimephales promelas Comments UV-treated Cone-% 1 UV-Control 0.6880 Non-Control 0.7360 10 7 0.74 10 21.4 0.7280 42.8 0.7240 85.6 0 7290 100 0.7050 Intake 0.6280 2 0.6140 0.7590 0.7590 0.7430 0.7530 0.7560 0.6900 0 5680 3 0.6530 0,6440 0.7280 0 7190 0.6750 0.6300 0.7310 0.6080 4 0.7630 0.6020 0 7430 0.7500 0.7150 0.7050 0.6940 0.6770 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max UV-Control 0.6795 0 9916 0.6795 0.6140 0.7630 Non-Control 0.6853 1 0000 0.6853 0.6020 0 7590 10.7 0.7428 1 0839 0.7428 0 7280 0.7590 21.4 0.7350 1.0726 0.7350 0.7190 0.7500 42.8 0.7168 1 0460 0.7168 0 6750 0 7530 85.6 0.7050 1.0288 0.7050 0.6300 0.7560 too 0.7050 1.0288 0 7050 0.6900 0.7310 Intake 0.6203 0 9051 0.6203 0.5680 0.6770 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal vanances (p = O 60) The control means are not ignificantly different ( -= 0.91) Hypothesis Test (1-tail, 0.05} Homoscedast,c t Test indicates no significant differences Treatments vs UV-Control CV% 9 3.22 10.871 1 711 1.914 4 494 7 683 2 618 7 306 1-Tailed N t-St t Critical MSD 4 4 4 4 4 4 4 4 521 1 943 0.0757 Statistic Critical Skew Kurt 0.95581 0 749 0 46261

O 5692 I.9538?.

47 4683

0. 11761 2.114691 MSDu MSOp MSB MSE F-Prob df 0.07567 0.11136 0.00702 0 00303 0.17896

1. 6 Dose-R spon e Plot 09 0.8 0 7 l 0.6 e o.s

(!) ~ 04 a ,.._ 03 0.2 0 1 0 p ~ Page 33 of94 ~ 8 i 1-tail. 0.05 level of s19ntficance

l J pecics:.;....:.;-'-=Je..:..:..:=-=-=:....c..:c..::.:.;;.=.:.:= Client: eguova h Nuclear Plant. ut nll 101, UV-treated Dally Che11,istr,*: Day Page 5 of7 ate: os-r-18 (Analvst identified for each dav, oerformcd 1JH, D.O. and conductivilv measurements only.) 0 J 2 Anulyst rlJ' ,-,J t15 Hr /-1.1' ,0 Conccntrntion Parnmcter 'tk,r., DH (S.U.)

,.9-:,

"1.~5

,. ~ 'Z

,_9,

'.\\.tnC DO (mg/L) 8.o ?.."1 (-:f.'1) 8. 0 '":{.c_ -:/. fj ~-~ Conductivity 30~ soe 311 co TROL (11mhos/cm)

All<alinity

~ -/* UV-treated (mgCaCO.;/L) \\.gt) ~~

llnrdncss g,,1..,

"~ !?'-\\ (mgCaCO.i/L) 1 "Temperature (°C) "--.\\-"'\\ i,~.'-\\ 1.J..\\., ""'L'\\* '!, "l..'\\.\\o """' * \\o pH (S.U.) "':(..9-=t. -:z_-::,13 -:/.98 -=,.-::re:;

/...ttn I'.)."1"6 DO (me/L) f:1.o

~ -"'1 R.o "':/-~ '::(.P,, 'l....t 10.7% Conductivity 293 ?.t:ts (umhos/cm) 2~1 "Tcmpcrnturc (0C) '\\.."\\., '"\\....'-\\,.'-\\ "\\..<\\- i ~\\~ i..'-\\,1 ""'&.t.S nH(S.U.)

f:-'18 7--:f.(;,

? -98 ""+-=!- I +-9Af l\\.~ DO Cme/L) f?.1 ~-0 R.o ':1.c, ~-a

j ~

21.4% Conductivity 29,1./ 7.":/-(s, Zrf (1u11hos/cm}

Tempcrnturc ("CJ

"-""* cf '\\.A.'\\ "'\\.'-\\ *.!- ""\\..'-\\*L- "\\.'-\\* 8 \\.'\\,i. pH (S.U.} ":l-18 -=,. f-'A '1,9f{ 7-.::;2

/.9J{

,..J.u<<;' DO (me/L) e., '8.o R. t ~ - "::I ':{..,, ~.2.

42. %

Conducth-ily 2 4(1 '2..4~ '2.38 (u1nhos/cm)

Temperature (°C)

-i..'-\\. f "-\\-S ,_'\\,K i..'\\,--\\ "'-'\\*'-' DH (S.U.} -=l,9S -=,.7.~ -:,.9~ -:;.~,L/ ~ -9Z '.+.-=.-?:i DO(ml!IL}

?.3 fll.o f=?.1 "7. 7: /

7..,, lo.Pi 85.6% . Contluclivil l":l~ /=fl 1'17 (µ mho~/cm) '"Temperature (°C) , -t.'\\ '"L'-l*,_ '1.'-1 *" "\\..~*'-' i}\\/\\ \\.~*'-\\ ' pH (S.U.) 1-9~ 1:=n. ~-9G, "":/.(,, s- ~. 9/ l:\\."i(1 DO (mg/L} ~.g B*< R.-z.. ?.1-Ftl.J lO,'"'.l-Conductivity /52 IL/(p l"-!tp (junhos/cm} 100% "Alknlinity 5~ S'-\\ (ml? CaC01/L) "'fl:trduc s \\a'-\\ (me CaC01/L) ~Q 4 ' ' - -

TR chlorine (mg/L),o.,o

-<-0.10

Tcmpernture (oC)

""t.<. 0 L.4.*b 1..'{.'1 '\\.'l-s 1.-\\* '\\ ~l-S. pH (S.U.) 7...493 1-+I "7/-. 9~ -:/.(;:,I.{ -:{. tJ-, '..J... ~. DO (me/L} 1?.~ 9?. Z Jf?. 2. 4 *1' 7.c, IO.Oi Conduclivil}' /50 (µmbos/cm) /'i:l 15-'f 100%

Allmlinity Intake (me CaCO;/L)

S\\... S\\o

1111rdncss l
,r -

(1112 CaCO;JL) l.,O loC "TR chlorine {mg/L) -<0.10 -<-0.10

Temr,ernture (OC) 1.':i*O 1.'-!*'l

'"LS, I ,. 'i *S ~-0 1H-1 Initial Fin11l Initial FinnJ lnilinl Finni 1pcralurcs performed at the time of test inu1a11on, renewal or terminnllon by lhe nnalysl 1dcnllfied in 1hc Dmly Renewal lnfonna11on table located on Page I. ... "Alkoh ily, 11ardncss nd total residual chlorine pcrli m1ed by the anal st identified on the bench sheet specific for each analysis and transcribed to this bench sheet ,.,.., L Y'.' 0

Totnl residual chlorine was performed on non-trcn1ed Outfall IO I and Intake samples.

V,...., age 34 of 94 SOP AT20-Exhibit AT20.3 revision 11 14

l Pa e6of7 Specie : Pimeplwles promeln Client: TVA I Scouo 1ah Nuclear Plant Outfall lOJ, UV~treatcd Dale: 05-15-18 Day (Analysl 1de111ificd for each day, pcrfom1cd pH, 0.0. and conduclivily mcasuremenis only.) 3 4 5 6 l nalyst Ii'--* P.sc., i:a.i::.c.... I)., i,l..l J-fS /-f~ rl..f Conce.n-Param ter trntion oH (S.U.) '..\\-.lt!> ":}~':l-1.oi+ 'l..cl ~ -4~

i ~,:;-

8.o'-( -=I,. 8D DO (mg/L) RJO "f,O i.l ,,,_q -:i. G., "':l-8 "':/.o Conductivity 3\\2 31~ !n'--' 298 (µmhos/crn) CONTROL

J\\lknlinity

~-~ ¥ I""'..-'" ~-Q, UV-treated Im~ CaCOJ/L) l.,~

t1nrd11e s i~

8-1

~

_01~ (m~ CaCOJ/L) l

Tcmncrnture("C)

._'\\,i "'\\..-\\.':,.. --\\., ""\\..'\\.::, ~-, "\\..-\\.* I.\\ 1..-\\*\\ "1..-\\* ~ pH (S.U.) e1'11 ""l.514- ~.os .ll..\\l~ 8ll'2. "':l. 'B"I 8,.nc::- ":{. "1B OO(mg/L) ~.o -::J.'! 'iJ.1 l\\.,o 1..~ "?.'-f --:,. Fl "':7. l 10.7% Conductivity ~ t.C\\\\ 2.it. (µmhos/cm) ~'l.l-

Temperature (°C)

.. -t-<f ""'""' '° i..'"'* &' "'\\-lo -v-1 * "1 "'\\.'f*~ "1.'(. &- '1..-l-S pH (S.U.) o0JJ\\ 1,5cii <:/,OIJ) ')-.,SO ao~ "?.es -oS ":[.y<j DO {ml'JL) 0.0 "::} <;7 'i. I u,,q '4-,":l- '?.,'-( 8-ci ~.. 21.4% Conductivity J.8.3 i-;w 2t.-=t (umhos/cm) ~~

Temperature ("C)

""\\_'-\\,! \\.'\\.lo ,..-\\. 8 ""\\..'-\\*~ 1.'-\\. \\ ""L'l -lo ,,.v, '1..1\\.\\, pH (S.U.) 911"\\ I '1.~, ~.otp ':\\-.SU, e.~3 "':/-.f!l'-1

8. <!:JG,

':/.R? DO (ml!/Ll ~. ~.&' <i.1 l,f-1 ~.,- ?-!! 8.o -=t. 2 42.8% Conducti\\*it

i'?>1-a~v 240

(µmhos/cm} ~64v qempernture ("C) .,.'l,'1 ~\\.~ ~'\\.( -'\\.'t. '-\\ '"'\\..'-\\,"'\\ w"'\\.l.\\.*"L -u\\.*" '"\\.'\\. s r>H(S.U.J 0.no -:}.--k., "i.0'3

~.-ts 8.03

..,~.: ?c, 1 8, 0"'(

"'=7-=T'-f ._Q£) (m,1./L) M\\ "'1. lo 'ii',l

~.o

~-B ~*'< 8-o 7.7.

85. %

Conductivity 104 i<I+ l-6 ".} J-:/ I (r1mhos/cm)

Temperature (*C)

'\\.'-\\*"I "\\..'-\\..1.o 1..'\\* C\\ ""\\.'\\,'-I ""* e "\\.1,VL 1.t.l,1 -'\\.'\\. "1-pH (S.U.) "1P12 '":J..'+~ 'b.t>'+ ll.~?, e.o~ ?.=,q 8-o~

J_ ':/'-J DO (mg/1.)

0.\\ '6., '.},.O 4.~ 7,3 8-a -:/.2 Conductivity ,s0i l~o lll>2.. /50 (iunhos/cm) 100%

Allmlinity lo'"L (mg CaCO;/L)
llnrdncs I

\\,.,,'-\\ i (mg CaC01/L)

TR chlorine (mg/L)

<-o. 10

Temperature (0C)

-u.o '1.L:, 1.c;. 0 1..l\\

S'

~ *t "\\..-t* 'i '"\\S. 0 1.~.1-pH (S.U.) '.1,.0,.C:, 1','S't "i".*'t ~-'1+ Bot -:,.. ~tt f:f.rt:>:J ":/.1~ DO Cml1/L) 0,7.. -:}-. " r.1

~11 i'.l,.e) t-8 e.,

~-1 Conduclivit

191 1si (lQ\\

J:;3 (µmhos/cm) I* 100%

Allmlinit,

Jnt.'lke (m!! CaC01/Ll s~

llanlncs (mg CoCO;/L) t.:.o
TR chlorine (mg/L)

,0.10

Temperature ("C)

,S.Q "l.~.lo "t.-\\,'\\ '"\\.4. ~ -'\\.C,..1) ~l.\\*S 1S *O 1.~-l.. Initial Final Initial Finni Initial Final lnitfal Final ';;,';:J;c, cratures pcrfonned at the tune oftest 111111011011, renewal or tcrm,nouon by the ~nal t 1dcnufied 111 the Dmly Renewal Information 10.blc located on Pa I. ...,.,L.a.Jl;;ill 1itY. hardness and total residual chlorine pcrfom1ed b}* the analyst identified on the bench sheet specific for each analysis and tra11scribcd 10 1his bench sheet ~ : . J"g' ref 'sj~ chlorine was pcrfonncd on non-treated Outfall IO I and Intake snniplcs. O SOP A TIO - Exhibit AT20.3, revision 11 14

l _........_~-. Page 7 of? pecie : Pimephales promelas Client: TV A/ Sequoyah Nuclear Plant Outfall 1 OJ, UV-treated Date: 05-15-18 Dnil)I C!le111isfr)1: Day (Analvst identified for each dav, pcrfonned oH, 0.0. and conductivity measurcmenls only.) 0 1 2 n:ilyst 6', LAS Mf AJJ ,-/(' ~ Concentration Parameter nH (S.U.) ,. ic.. --:,.e3, f* ?/9 ":f.:r:s

71. 6'~

~.<.Pl Control DO (mg/L) '.<:\\ 8 -o -:/--:/ +-C:, +.8 "'. 'Ji on-treated Conducth,ity (11mhos/c111) 3 \\t.- 30$" 302. ",\\ 11.alinily to\\ ~ -"'"' \\..,C) (mg CnCO;/L)

11~rd11c g1-

--~ !l '-\\ (mg CnCOJ/L)

Temricraturc (OC) 1..'-1..g

'1.'l, ~ ,.A.\\. "\\.-.\\. --\\ ~'-\\ -b "\\..'"'*'° Initial II Fin:il Initial Final lniti:il II Fin:11 Da (Analyst idcnlificd for each day, pcrfonned pH, D.O. 1111d conductivity measurements onlv.) 3 4 j 6 Analyst ,I'- &;L, &;v t..l ~ 1-1~ f/f/' J-1~ Conccn-f'nr<1metcr trntion MJ'b5-iut6 pH (S.U.) 'l-.~Oi -:J..5 1 S.o'+ /+.'4~ 1,. f: I ~.:,'i "7.f!4 ontrol DO (mwL) l).q

J,,(p

"[s,O "4-..0 0J.)3) ~ -~ '?.-z. i :':1 ":I. I on-treated Conduelivil * (11mhos/cm) 31'5 3o"a joo 1ot;"

Allrnlinily

~ ~, \\ (.p \\ '\\_'IY,\\ (m~ C11CO;/L)

llnrdn s g'-\\
~

"~ (mg CaC03'L)

Tcm1>crnturc (°C)

,A-'1 ,.~. s 1-'4-1 i..'-\\-~ "l.'-\\. lo "\\."\\-s i..'-\\.. ~ i.{, lo lnitinl Finni I lnitinl Finni fnitinl Finni I lniti:11 Finni

Temper111urcs perfornied 111 the time oftest initiation, renewal or termination by the analyst identified in the Daily Renewal lnfornmtion table located on Page I.

Alkalinity, lrnrdncss ond total residual chlorine performed by lhe analyst identified 011 thc bench sheet specific for coch analysis and transcribed to this bench sheet by: --.---* Page 36 of94 SOP A TIO - Exhibit A T20.3, revision 11 14

Enyt,onmcflt31 Tostlllg SoluUon,, Inc. Con<<ntrntion Pn 111 trr 1111 (Slll DO (m~/1, ) ~outrol. Co11duc1,vit1 l11mhw,/c111) No11.. trcntc1I /\\llrnlinil)* (111~/L CnCOJ) llnnlnl'>s (mW). c:~co,1 I tmncrnturc ("C) 1111 (Slll DO (rnt!/1.1 ("ont rol, Contluclh*itv (umhos/cml 11v.1, ~atcd li\\lknlini1y (n,1(/L C:nC'O,) llur,luc~~ (111j!/I, C11CO,) Tc11wcn1turc (C) 11111 (SUl DO /m~/LI 111.7'\\i, Conducll\\ifY (u111ho,/c111l ' rcm11~1~1turc (C:> lnll lSUI 21.4% DO(tn!!ILI Conducln ii\\ (11111lm,lc111) 'l'cn111crntu~,*c, lnll lSU) 42,H'Y* DOtnwll,l Conducthit1 (umltos/cml Tc11111eiit111rc ("Cl 11,B (SUl 8S.6'Y* l>O trn!!IL\\ IConductil in* turnho,Jcm) Tcm11cii11Urc (C) 11111 ISlf) DO(mu/LI " umlurth it1 (11111ho,/cml 10(1% All,alinity (11111/L C11CO, ) llnrdn~, (tni:/L CaCO,)

ro1:1I He,idu:,I Chlo, Inc (m~/L
r1:m11l'r,11urc !"Cl nil (Sl' I 0011110/Ll Coutluc1M11 (1u11ho.,/rrnl IIMJ'.. lurnhc 1\\llmllnlt) (mwL CHLOJ) lli,nJnc'" (u1i:/l. CnCO, )
Tomi Ht.,itluul Chlori11c (ruu/L Tcm11cr:i1urc ("C l TVA/ equo ah Nuclear Plant, Outfall 101, V-treated Ma 15-22 2018 Pimepltnle. prome/11 Chronic Whole Efnucnl To icit T t R-02-0 13, Method 1000.0 Dail hcmical Annly e Un,* U 0~1 I n.,,1 OoyJ lln1* ~

Initial Finni lniti;il n11ul lnllial l'inal luilinl r,in:il lnllinl 7 86 7.8 1 7 89 7 73 7.85 7 ss 7 '19 7 5 I 8.!J.l 7.9 so 77 76 7 8 6.3 79 76 80 312 305 302 3 15 J08 6 1 60 61 82 M4 ~l 2H JJ J 2H 24 <> Nll .!4 7 14 5 !41 797 18:5 7'15 78:? 7 'II 765 19~ 7 67 8 t\\.l 8 (l 79 8.0 76 1R 74 8.0 80 8 I 309 308 31 1 312 J l8 611 59 63 81 84 84 141 24.4 lJ 7

n 24,6 24 I, 2H 14 5 l-l?

797 77~ 7 9X 77j 7.93 758 8 01 7 ;;4 II.OS 80 79 80 7.6 7.ft 7 4 8 () 79 81 19 !'15 29 1 300 292 .4 7 24 ~ 14 S 214 24 7 24 5 lH JH 2H 7118 7 76 7\\18 771 H -1 159 801 759 8.116

8. 1 80 30 7.6 78 7J so 7.S l! I 174 176 277 183

!78 148 24 4 24 \\ 14 2 14 S 2n '2H lH -H 7 9R 7 7ll

79ll 171 7 94 765 801 7 61 S\\16 8 I 80 3 I 77 79 S 1 7.8 81 241 244 2J8 237 2-16

_4 ~ 24 5 148 14 ~ 2H N 4 14 y ~4 3 -H " 95 7 73 796 76-t 7 91 7 53 801) 746 805 s1 8.0 8 I 77 79 bB SI 1.b SI 174 171 177 IS~ 18.J 24 I} 14 2 249 1~.J 249 24 J 24 Q 146 .4 9 7 9-1 772 79~ 7 65 7.9 1 7.50 H:? 7 46 Kt1.J 83 8 I 82 71 8.0 t, 7 SI 76 SI ISl 116 146 159 1(111 5~ 5-l t,1 (;(I 64 f!,1 010 0 10

0. 10 25 U 1H 149

!.J 5 249

_.J,5 15 fl _.J) .:!5U 1 '13 7 71 79.J 7.6'1 7 89 7.ll 7% 7 5.J 8 ().l 8.3 81 S.2 77 79 c,*9 8.2 7.6 8.1 150 141 15J 1511 ISS S6 51l 51, 60 611 I,() ,o 10 010 ti 1U 25 0 !-1.J 1, 1 ~.J 5

!SO

.!4 7 15 0 14 t, _J 9 Nc,p fot I

rduul chl<mnc was rcrformcd on nn11-1rc.11 d Ou1falf 1\\11.. J ln11kc *.impl
Fi1111I 749 70

_4 4 n4 (i 9 24 3 7 -19 70 ~46 7 50 69 ~.j 3 756 69 !4.I 14S 70 24 J 7.J3 70 2-1 J H7 7.0 ~43 l'rojccl nnn,bcr: ______ 1_3_J3_7 Dn,* 5 lnitwl Finni 803 7 81 76 72 100

!H 24 S 7')(/

785 1b 76 10.J 2n 14 4 801 7 lW 77 7,j 29 1 1n -* 3 S 03 7.85 n 7 4 274 24 7 24 6 s llJ 7.84 7.3 146 l -1 7 _4.J 8 03 1.16 7.8 7.3 187 )48 2H SOJ 774 77 73 162 2H 24 4 81).j 779 H 7.J 161 .!S V . 4.5 llnv 6 lnitlsl Flnnl 7.99 7.84 77 7.1 JOl 2-18 1~ () 8 O.J 780 78 7.0 196 2n 243 8 05 7 78 78 7 I 286 1-18 24.! 8 OS 7 7() 80 7 I 167 24.9 14 6 8 06 781 80 72 240 N9 HS 8 04 774 80 72 171 24 9 .n 8.0.J 77J 8,0 7.1 150 1!0 NJ 8.03 77.J 8.1 7 I 1}3 150

N l File s n10 _051 51Schem Entered by T Sleeper Reviewed by -tJ:{--

Pagel of7 Ch roni Whole, ffluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriotlaplmia dubia lient: Tennessee Valley utbority Facility: eguovah N uclear Plaut NPDE #: TN0026450 ProJect #: Dilulio11 prepal'atiou i11(or111ntio11: Diluuon prep (%) 10.7 21.4 Effiucn1 volume (ml) 267.5 535 Diluent volume (mL) 2232.5 1965 42.8 1070 1430 Totnl volume (mL) 2500 2500 2500 Test or}!nnism source ill(ormation: Or!!anism a!?e: < 24-hours old 85.6 100 2140 2500 360 0 2500 2"00 County: Hamilton Outfall: 101 Comments: Test i11formntio11: Randomizing template color: Date nnd times organisms were born o<;.,c;-\\f C>l.,QO,i. IO'-\\ lo Incubator number nd shelf between: location; Culture board: US-o&*\\k A_...........-0S-oi*18. & Replicate nur,ili~r-i I ! i. ~ ~ I s I 6 I 7 I s I 9 I 10 Culture hoard cup number: ~':> I~ I J1 1 J-I \\"'LI I:'> I I'"\\ I ~ l..x> 13\\ YWTbatch: Transfer vessel i11fomm1ion: I oH: 1,'\\~ S.U Temperature = "\\..I.\\.~ QC Sele11ns11*1,m batch; Avcrnec 1mnsfcr volume (mL): I < 0.25 ml Daily renewal i11for111ntio11: Day Date Test inith1tion and feeding, MHSW S:1mple number used renewal and feeding, or batch u ct.I utfall 101 Intake termination time 0 05-15-18 llO~ O':.*lO*\\f A, l f( OS l'*l.Ui... \\ q OSt'-1, lf"i l 05-16-18 ll"\\t'\\~ ~

lO"'\\~ (\\

l( OS ti\\. 01.. \\~cc:-'* ~ 2 05-17-18 \\Ol~ O~*\\lc.--\\if\\ 180Sl'i>. o'-\\ \\.i-1.)S I~, 0$ 3 05-18-18 ll.) :r'\\ t),S,\\lo*t.k " \\iO~tlc,. o'-\\ ,eosib.o-; 4 05-19-18 \\t) "'l..\\ os.*1b*\\ a~ 1 i os,~. l>".:> \\~0~\\~ -~,1 5 05-20-18 ,cs-i., O~*l\\.*18~ 1, !\\C I 5(, O"". \\ROS\\J. o'-\\ 6 05 18 1001.o D~*\\\\:i* liB \\ &~S\\i., CP.:> ,&-~ \\!, o-; 7 0- 18 \\.t:i'\\.. S i\\u.JP 1-~l c,.\\ -1\\,- l i° o+3o-,, Analyst Y\\

1 Jf -*u

~, V' \\,l 1A,, Co11trol ill/ormntion: St1111111mJ1 of test endpoints: Control-I Control-2 Acccpt:incc crilcrin % of Male Adults:

07.

0.1 ~20% 7-day LC;o > ID01. % Adults having _,,d Broods: lnD 1-lOC> 7. <!: 60% surviving adullS NOEC \\DCI 1. % Mortality:

01.

S20% LOEC >1007. Mean Offspring/Female: -U.4 1..'i.. 0 <!: 15.0 offspring/female ChV ') LOO 7 %CY: \\o. ~..,. s.~1. <42.0 % IC2s '> 1001. Page 38 of94 SOP A Tl I - Exhibit ATI 1.2, revision I 1-01-14

Page 2 of 7 l Species; Ceriodap/111io,tubia Client; TVA/ Sequoyah Nuclear Plant, Outfall LOI Date: 05-15-1 CONTROL-I Survival n11d Reproduction Dntn Replicate number Dav .I 2 3 4 s 6 7 8 9 10 I Young produced 0 /) {) /"', (") 0 () () () n Adult mortality L L... 2 Young produced r, ('""\\ /) (') r, n 0 0 0 0 Adult mortality L L. '- \\_... \\._ L... \\._ ~ J Young produced (\\ n 0 0 0 0 D

O 0

() Adult nwrl:ilily L L... '-- L-l.._ 4 Young produced '-\\ '-,\\ ~ --\\ ~ 5 ~ ~ -~ Adult mortalrt}' \\._ \\.__ L 5 Young produced ~ \\\\ IC) I\\ '~ \\0 lO \\0 \\ L- \\ 'b Adult mortality \\.. 6 Young produced () 0 () b 0 D 0 0 n 0 Adult mortality \\._ '-- \\- \\._ \\._ L \\.._ L, l_ 7 Young produced \\ \\o \\ => \\ => :\\ \\\\. \\ '-\\ \\ "L,s l '-\\ \\I.\\ Total young prod1tccd (,. <a "'2..~ "'2... "1.,_ '\\ "'2..~ L..\\ '"'l..fs '"2... '\\ ""L \\ Final Adull Mort:tlllY L ~ X for y d Broods -,c;._ )<. ")o(.. '-../ ~ )< '>(_ -x- ~ 'V NOi.: M<tll mortality (L

r.,.._ D
d~d).SEl
spl' bcood rsi!l',ik btood,p~l bct,<<<n 1wo d>ysl. CO
c,,ry o**tr{cluprin~ corricd oscr will odult Jurin* lran,r<r)

Co11cefllnrtion: % Mortality: I f"l1. Mean Offspring/Female: I -U,-4 CONC: l0.7% Survival ruul.Reproductio11 Data Rcnlicntc 1111mbcr Day I 2 J 4 5 G 7 8 9 10 I Young 11roduc~d o* 0 0 0 0 n {) 0 D () Adult mortality L \\._ \\._ '- L L L '? Young produced 0 C) 0 C) 0 (j ("\\ () () T'\\ Adult mortnlity L L L L L L L 1....... J Young produced fl {) 0 0 0 0 C) 0 0 0 Adult mor1:11i1y \\._ '- L l__ \\__ '- L \\_ 4 \\'oung produccll ~ 45 '-l '-\\ s ~ I..\\_ ~ ~ ~ Adult morlality L L \\._ L L L 5 Young prodt1ccd \\<.. *~ \\ :, l"'l... \\Q I (:) I-,_ \\\\ \\() Ii.... Adult mortaliiy \\._ \\.._ \\..... \\.._ 6 Young produced /) 0 0 (') 0 0 () 0 0 D Adult mortality \\_ \\_ \\._ L \\_ L L \\._ L \\._ 7 \\'oung produced.~ \\~ t '$ 11.. I'-\\ l I... \\\\ \\ ':) lR I'""\\ To1al you11g produced ~, "°!):,

!> '1..

~1. "l." "2." ~!) "'2...'1 ~ \\ 3'"L Filial Adult Mortnlit,* L l_. L... \\...._ Nott: Adulr mon:,lit (L - 11,*c,. D

dt'.:,d), SO :a-splh &rood (M.t,leh~ d split brtwcen l\\\\'O dn.y$), CO* ctn\\' over (offiprin~ e.trrie<t ovc( uith ltduh dunnr,. 1ra.n.sfc::r).

Co11ce11tmtio11: % Mortality:

07.

Mean Offspring/Female: 3 \\.\\ % Reduction from Control-I: -'\\-~ l. Page 39 of94 SOP ATI I - Exhibit AT! J.2, revision l l-01-1 4

~ ----- l Page 3 of7 Species: Ceriotlnplmin tlubifl Client: TVA/ Sequoyah Nuclear Plant, utfall 101 Date: 05-15-18 ONC: 21.4 % Survival and Reproduction Data Rcnlicntc number Dnr l 2 3 4 s (i 7 8 9 JO I Young protlucctl ri 0 0 {) t'I 0 {") 0 0 0 Adult niortnlity L \\...- L. L 2 Young produced 0 ~ () n 0 ('\\ (') t) () 0 Adult mortality \\.._ L L. Young protlucctl ~ (') 0 0 0 D 0 0 n () ,\\dull mortality L L. L L. L-i..._ Youug protlucctl c::. '-\\ ~ ~ s '-\\ '-\\ '-\\ ~ '--\\ Atlult mortnlily \\.._ \\... \\.._. Young protluccd '\\ 1.... \\'"L \\ I,'.) IQ I 0 \\-:':> '() \\ \\ \\~ \\~ Atlult mortnlity L- \\_ \\..... \\._ G Young producctl (') 0 r, 0 r,, b 0 C) (\\ 0 Adult mortnlity L. '- L. '- '- \\.._ "\\.._. \\.._ 7 Young produccll '\\o \\ \\a \\~ \\\\.c l <=:> \\ \\o \\S \\'\\ Total young protluccd =,'-\\

>"1..

~b =, \\ ~, ~ "1- -=>t> 3,t) ~'4 3\\o Final /\\dull i\\lortality ~'Ole: Adull monolny (L

Irv D
dod). SD
1))111 brood (soosJ* brood 1plit bttw<<n two d*)'s). CO
c:ir,y

( r, ovc.r ollspnnc CJ.m h I ewer,,,1.idu t dunnc 1uns er) Co11centrntiofl: % Morlality:

01.

Mean OITspring/Female: 31..Q % Reduction from Control-I: -n.., 1. CONC: 42.8% Survival and Reproduction Data Rcolicntc number D~y I 2 3 .j 5 6 7 8 9 10 J Young produced 0 n (\\ () () () (\\ () 0 0 Adult morllllity L. '- \\._ \\. L-2 Young producetl ~ 0 0 (') () () 0 0 {) 0 Adult mortnllly \\..- \\..- L. \\.._ \\. \\.._ \\........ \\_ 3 You11g protlucctl 0 0 D () () 0 0 t) () (') Atlull mortality L- '- L L L L \\.__ 4 Young protluccd ~ s Ll ~ '-\\ s ~ '--\\ '-\\ \\.\\ Atlull m rlality L. \\.._ L L \\..... s Young produced \\:) \\ \\ I ~ 11-l L\\.

10 I\\

l I!) Atlult mortnlity \\.... '- \\.._ L L L G Young protluccd 0 (', 0i 0 l) n 6 {) 0 6 Adult mortality L L L \\._ L L L \\._ 7 Young protluccd I I., I'\\

S 11

,g ,g q Ho I (s I\\ Tomi young protluccll ~"1.. ~s ~2- ~, ~'-\\ ~\\ 3'-\\ ~ t:) !>~ ~\\ Finni /\\<lull Mor*tnlit)' L. '- ~ '- \\.... ~ '- N111~. AdulC n10n.uldy (L

li\\'t. D
dtJ.d), SB
spin brood {11ns;tc broods 11 bttwttn two d.1y.st CO
arr, o,cr(otrsorin1! a.med ~tr,,,.h,aduh dun.M uoosftrl.

011ce11trlllio11: % Mortality:

01.

Mean Olfsprin.g/Female: % Reduction from Conirol-1: -\\i-o 7. Page 40 of94 SOP ATI I - Exhibit ATI 1.2, revision I 1-01-14

Page o 7 Species: Ceriodllpl111ia,tubia Client: TVA I Seguovah Nuclear Plant, Outfnll 101 Date: 05-15-(8 CONC: 85.6o/o Survival mu/ Reproduction Dot" Rc111ic:11c number Dny I 2 J <I 5 G 7 8 9 10 l Young produced 0 n D 0 0 0 n () 0 n Adult m rtality L-1 Young produced (") 0 0 0 0 () 0 0 (') n Adult mortnlity L \\..._ J \\'oung produced (<') 0 0 0 D D a t\\ 0 D Adult mortnlity \\._ \\...._. L- \\__ L l.._ 4 Young produced '-\\ \\. lo "-\\ 5 '-\\ '...\\ \\o 5 Adult rnorrnlity \\...... \\...... \\__ '- \\.... \\.,.... L. 5 Young produced \\ ""'2.. n.. \\ "\\_ \\ :> 1 '-\\ \\ L. I C) 10 . \\~ Adult morcnlity L \\...._. 6 Young produced C) 0 D D 0 D 0 D a a ,\\dull mo1*1:1li1y \\_ \\_ \\_ '- \\.... \\_ \\._ 7 Young produced L.0 \\ A- \\\\ ,g ""1.,.c:, \\ \\.o \\S ,<\\ \\" \\\\ Total young produced

,\\:,

"'::> lo ~c; ~s oC\\ -~~ ~\\ ~'-\\ ~s ~ I Fin~I Adult Mortality ~ '- '-= \\_ Nott : Adolt mor11o1lity (L

lfrc. O.:t. deJd), SO
split b;ood (sinyle brood spin bcl,,.~cn 'WO ti:i,J\\ CO* c.:tr:

over (01Ts:uri1111 c.arricd ovc, '"'".1.dull du.rirut 4G1nsfcrl. Co11ceflfrntfo11: % Mortality:

01.

Mean O!Tspring/Pemalc: ~ '-\\. '\\ % Reduction from Control-I: - 2 1.-'\\ 1. CONC: 100% Survival mu/ Reprotluction Data Rc11llcn1e number On)' 1 2 J 4 5 G 7 8 9 10 I Young pr'oduccd n _o_ i) () n 0 0 0 0 0 Adult mortnlily L \\_ L L L.- L. L L 2 Voung produced i) 1\\ 7'\\ (\\ L) l"i (') c\\ 0 0 Adult rnorrolity L L. L. L L L L L J Young produced (') 0 () 0 C (') 0 0 0 0 Adull mort. lity L.. '- L L L L L L -i Voong produced 'S ~ s \\o 'S ~ I.a '-\\ 5 ' Adult mortnlily L L L_ '- 5 Young produced \\ -.\\ \\ ' l~ \\..,_ \\~ 14 i "'\\ 1-Z... \\ 'l_ \\~ Adult morrnlity \\..... \\.- L G Young produced ("\\ () () () () () (\\ 0 () C) Adult mori:ility \\... L L L \\._ L L L L 7 Young produced \\ ~ \\ i lt\\ "l.. \\ \\~ L. 0 ll \\ ~ ~\\ \\q Totnl young produced

,i

~:> 31 ~ '\\ ~ \\. ~'\\ ~b "?I'-\\ b \\.\\ ~. final Adult i\\lorlnllty L L. L. L L L L \\ L. I.._ No, Adut1 mon*ltty (L l"-c. D dc,d). SO

split brood (s,ni;J. b,oocl sp~I b<t"roo 1wo d*yi), CO *g o,tr (ofT, n*~ c,mcd Ol'<t wuh aduU dunn~ tr.11uf<1l
Co11ce11lrnlio11:

%Mortality: Mean Offspring/Female: % Reduction from Control-I: Page 41 of94 SOP AT I I - E.'hibit AT! 1.2 revision 11-01-14

1 Page 5 of ? pcdc : Ceriotlap/111ia tlubin licnt: TVA/ Scguovah Nuclear Plant, Outfall IO] Date: 05-15-18 0 TROL-2 Survival mul.Reproductio11 Data Replicate num ber Dav I 2 3 4 5 6 7 8 9 10 I Young produccll (") /") () 0 () 0 () n /') {)

\\ dull mor1alily L

\\_ L-L- \\.._. \\...... \\,_ 2 Young produced l) 0 0 0 D c) (') (1 r,, n AClull mortnllty \\_ '- \\__ L.. '--- L. 3 Young produced 0 0 0 0 D D 'C) 0 t) 0 Adult mortnli1y \\..._ \\.._. L. \\_. L \\..._ '- L 4 Young produced L\\. '-l "!) "-\\ ~ ~ ~ ~ ~ "-\\ dill! morrnlilJ' \\._ \\_. \\,_ \\._ \\_. Young produced \\ Cl I ?_ '\\ \\ "L 10 I Cl ,o \\ \\ n_ I \\ Adult morlnlily \\.... \\.... G Young produced () 0 0 n 0 0 0 0 0 D dult morrnlity L L \\._. \\_. '- L- \\.._ 7 Young produced \\ \\.o l~ l'-t I'-\\,, \\ lo \\\\o \\~ I '-\\ '~ ro11l i*oung prod uced

,Cl 1.."\\

'2.. lo DO ~ o "l'\\

)

""2.i oc "1..8 Finni Adult Morlnlity \\._.. \\_. X for 3,d Broods --.,c. -)C ':,{_ ~ 7- -y._ 'L ':I-NUI,. Adult man lily (L

Ii,,:, 0
d J), SB
spti, brood (s,nglc brood spl11 bcr~= 1wo da,s), CO
c:,ny ovct(o!Tuprin c;urird o*.. rnilh adull d"""' tr.ansfn)

Co11ce11trntio11: CONC: 100°/c I 0 nta <.e S . I I R I I" D t 11/'V/ V(l lllll eprori uc 10 11 na Replicate numbtr 0:J) I 2 3 -I 5 G 7 8 9 10 l Young protluccd (j n 0 0 0 () () 0 0 0 A<lult morlnlity L \\.._. \\._ L L- \\_. '- 2 Young producctl 0 D 0 D {) () () 0 () 0 dult mortnfity L \\,_ L \\,_ L \\,._ J Young produced (') 0 D 0 0 0 0 0 (') 0 Adult mortality \\_. \\_. \\._ L \\._ L L \\._ ~ Voung produced s '5 "-\\ s s lo '-\\ 4 l...o Adult mortnlity L L L L \\,_ L \\._ L L- \\... Young protluccd I "'L \\:;, \\,_ l \\

12)

\\1-10 IC) 13 (C) Adult mor1nli1y G )'oung protlucc(I 0 n (') {) n () () D 0 0 dull mor1:ili1y L ~ '- L L \\... 7 Young pr du cd \\~ \\S l ""\\ \\ & \\~ \\ "\\ \\ "" \\ '\\,, l~ Tolnl young producctl ~ ~ ~~ ?)~ ~q ~"' ~lo ~ "1.. ~~ :1'\\ ~'S finol /\\tlult MorrnlitY L- ~ '-- \\..._ ~ L- \\._ \\._ ft'IJk, Adull monafit (l

lin*, D
dad). SB
l,t brood 1u, It: brood $plit bctwcr::n 1\\,-o d.J s). CO
c:ur O\\tflofTsorim:. c:1mcd D\\ c.r,.. irh.aduk durina tl'l1.nsfcrl Co11cc11trntiu11:

% Mortality:

7.

Mean Off: pring/Female: % Reduction from Control-2: Page 42 of94 SOP ATI I - Exhibit ATI 1.2 revision 11-01-14

~ CQ C1)... ('I) 0..... f [J111l,*nm<<nlf'J Tetlf!'lt Sntutln.*n, ll'IL. Doy I 2 3 1 0 0 {1 1 0 0 u l 0 0 (l 4 J 4 J 5 () II Jl) 6 0 u 0 1 Ill i'.I 11 Toiol lS 2S 27 1);1' I 1 J I 0 0 0 l 0 0 0 3 0 0 0 4 4 s 4 s 12 10 IJ (, a 0 0 1 IS 18 IS Toto! 31 JJ }l o~l' l ~ l I 0 II \\J l il 0 0 J 0 0 0 J j J ~ s 11 I! JO 6 I) 0 a 7 17 lb 16 l'oUif J4 JJ JO 42.8% lhj I 1 J I I) 0 0 l 0 II 0 J 0 ll 0 4 3 s 4 s ll II ll 6 0 0 0 l 16 ,o u r..., )l J~ 3? llt 11ic~Ut. n1111,t.u~ri 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 J 4 4 s II 13 10 10 0 u 0 0 15 lb 14 12 29 JJ 28 27 f<c tlirll f(' numbl'r 4 s 6 7 0 0 0 0 0 0 0 p 0 0 0 D

l J

4 12 10 10 12 0 0 0 a 16 JJ 16 i7 Jl n 1') Jl R1: 11ic:ak' 1l11.n1btr ~ s 6 1 0 (l 0 0 0 0 0 0 0 (l 0 0 J 5

I 4

10 10 IJ 10 0 0 0 0 18 16 IS 16 JI J I 32 lP Rcnlluh.* numt,e-r ~ 5 (, ' t) 0 0 0 0 0 0 0 0 0 0 0 b 4 5 11 12 I~ I? 0 0 0 0 Jq 18 IS 17 37 JJ J7 J~ TVA/ Sequoya h Nuclear Plant, Outfall IO I Mny 15-22, 20 18 Veritlcntion of Ceriodaf)lt11ill Re1>rod11cfion Totals 8 9 1(1 fOIAI l)uy I 2 J II a 0 0 I (I 0 0 0 0 Q 0 l 0 0 0 0 0 JJ 0 J 0 0 u l J J 1(1 4 ,I b 6 10 11 10 106 s ll 12 11 0 0 0 C) b 0 0 0 15 14 JI 141 7

o IH 11

?8 29 21 l8J I 0101 Jf, Jli J5 100% 8 9 10 Tomi llo\\ I ? J 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ) 0 () n ] 3 l 38 4 s I s II 10 ll In 5 14 11 D 0 0 0 0 (, 0 11 0 JS IS 17 ,~, 7 IS 'S 1q 29 J I 3? J II -r.,., 37 Jl 37 Control-2 6 ~ l!I Tolnl D,y I ? 3 0 0 0 0 I n 0 0 0 0 0 II l 0 0 0 0 0 0 0

I 11 II

{I 4 5 4 ,n 4 ~ -I J 11 11 I~ I 14 -~ 1u ll ** 0 0 0 0 6 0 II 0 u 16 1'1 I<>* 7 lo,. iJ JO H JG J?O l'o(l11 JO 19 36 ~ 9 10 l'*t~I l>Al I l ) 0 0 0 0 I i) Q 0 0 0 0 0 2 0 0 0 0 u 0

1) l 0

0 0 4 ~ 4*1 J ) 4 JO II 10 118 s 12 11 I! 0 0 0 0 G 0 0 0 Jo 18 17 173 7 J[ Ii n JO JJ JI JJ5 1"tU \\\\I J:; ll 33 Ucnlltal\\' numl.to.r ~ 5 6 1 0 0 0 0 0 0 0 0 II 0 tl 0 4 s 4 I) 14 13 ll 0 0 0 0 ,~ !O '" 15 J5 39 JJ JI ft t!- >fktalt nulllht"r ~ 5 6 7 0 0 0 0 Q 0 0 0 0 0 0 0 5 5 6 ll IJ 14 13 0 0 0 0 ! I 18 20 17 JY 36 J? J6 R.: 1lic:1tc ftl1HJIJU 4 5 6 7 0 0 0 0 0 0 0 0 0 0 0 0 ~ 3 J s 12 10 10 IO 0 u 0 0 17 ]6 16 lll Jfi 19 31 nc lllettle number 4 5 G 7 0 0 0 ll 0 u 0 0 0 0 0 u 5 ) 5 6 II 13 I? Ill I) 0 0 0 JS Ill 10 I(\\ JJ J4 36 J1 8 ~ 0 0 0 n 0 0 s b 10 Ill u 0 I') JQ J4 JS ~ ~ 0 0 0 u !) 0 4 s 1.2 I~ 0 t) IS 17 34 34 N ') 0 f) 0 !) 0 0 J I I 11 0 0 IJ 14 27 JO 8 V 0 0 0 0 (I 0 4 I JO 13 0 0 P) 17 JJ JJ JO tl 0 0 1 13 0 17 JS Ill 0 0 0 5 13 0 19 J7 Ill f) 0 0 ll 0 IJ 28 10 u u 0 6 IU 0 Jq )~ °fMAl Q 0 0 49 121 0 17? ) 49 fol~f 0 0 0 50 127 0 ISS J6l 'To1ol 0 0 (J 1()7 0 14(1 1'111 1ulnl p 0 0 47 116 (l 116 JJ9 *---- 7

~ CQ CD t 0.... f Envi ronmental Testing Solutions, Inc. Conccntr:otion (%) ontrol - I 10.7% 2 1.4% 42.8%

85. y,,

100% Control* 2 100% lntul r 0 11,ruu 1111: Dunnctr's,\\I, I) valur; l'~I 'l>: ln tul c: I ll llltNl 's M I),1~l11c: l'M 0 : I 28 ' I 4 2 36 37 30 35 2 J 21! _7 )3 32 32 30 5 2 3S 33 37 2 2

3)

?.0~7 7.2 1.080 3.7 T A / equoyah Nuclear Plant Outfall 101 May 15-22 2018 hronic V hole

ff1ucnt Toxicity Test (EPA-821-R-02-013 Meth d 1002.0)

Ceriodapl111ia dttbfo hronic Whole I fflucnt Toxici Tc EP R-02-013, Method 1002.0 Quality Control Verification of Data Entry, alculation, and tati ticnl naly cs l'rojtct 1111111btr; ___________ JJ_3_3_7 Re 111irnle nu1nbc.r Sun:iv:,I 1\\ crll!lC reprod uction Cormrit11t ot Prtcu11 r d11 1101, from V. ) 1orf pri nit/rcrn11lcl uartnlt u (Y*) potlltd con1ral t ,I 5 8 9 Ill 2 3 1~ 27 28 29 27 ttiO lSA 6.3 Not npplicuhlc J2 19

21) n 29 31 31

,oo J I. I S.I -9.5 I 3 I ]:! 0 0 3~ 6 100 n.o ,A -12.7 J7 3~ )7 34 30 33 JI 1(10 33.S 7.t -111.0 5 ') J JI 34 33 3 100 3-l.\\l 6.0 -22.\\1 <) 3b JC/ ]6 34 34 37 100 ,.:? 5.6 .z1.s JO 30 2\\) 31 27 28 100 29.0 o\\'ol 1lrtJ1lic:1bl ~ 4 J(, 32 33 34 35 1()11 33.!> 3.S -16.\\1 M. D: M111111w111 1g111lic:u11 Dillcrcncc l'M 'D = Percent M1nm1um S1gn,licant Differ nee l'MSO 1s o 11cas111c oftest rrcc1s11J11 l'hc l'M 1s the 111111,mum pcrccm dincrenc l1etwccn the conhol und tre uncnt Llml cnn I dcclnrcd musucall) s1 *111ftcmu III a I hole cfllue1111ox1c1ty test I owcr PM D bound dcu:n11i11cd by U l:l'A (10 11' pc1ccnltlc) = 13% Upper PM D b(,und dcterrr.mcd by U f:.JlA (90'" pcrccnl1lc) =47% l.o\\\\'cr nnd upper PMSD bounJs wcrcdctcnmnccl l'iom the IOU1 and 90th pcrecnlllc. res cctivcl. 01 PMSI) d 10 finm EPA' WET lnterlnborat I) Vnnab11tt Study ( Sl;PA, }1)(11, SEl'A. 2001b), ) Shi'/\\ 200 1a, 211 lb hnul Rcrmn l111crlnhont1nl)* V:1r111hih1y tuJy nt t,P/\\ Sh11rt-tcn11 'hro,11~.ind /\\cntc Whole lffOucnt T11 octl r.:. t Mdhod!., Vnh1111~-,; I <1111.1 2-1\\ppcndts. El'A, g11.u-0 1-0tH.1nll EP/\\*81 1-0-0 1-005 US E11v1ronmc11lnl Prou:c11011 /\\,:ency,

onomnnll, 11

Start Date. 5/15/2018 TV / equo *ah uclcar Plant Ma J ~-22 2018 tatistical Analyse utfall 101 Conodaphnia Survival and Reproduction lHt-Roproduction Test ID CdFRCR Sample ID. TVA f SON Oulfall 101 End Data 5/22/2018 Lab 10 ETS-Env,r Tesuno Sol Sarni)! Type DMR-D19Ch,ga Montto rig Repor, Sample D le. May 2018 Protocol FWCHR-EPA-821* R*02-01 T t Spaoas CO-Cenodaphnta oubla Comments* Non-treated Cone-% 1

z a

4 5 6 7 8 9 10 Control-I 26.000 28.000 27 000 29000 33.000 28.000 27 000 28 000 29000 27 000 Controt-2 30.000 29000 2.000 30000 30.000 28 000 31.000 27000 30 000 28000 107 31.000 33000 320 0 32.000 29000 29 000 33.000 29000 3 1 000 32 000 214 34.000 32000 30000 31 000 31 000 32,000 0.000 30000 34000 36000 ,2.8 32.000 35000 32000 37 000 34.000 37 000 34 000 30.000 33 000 31 000 856 36.000 36 000 35 000 5 000 39 000 33.000 31.000 34.000 35000 35000 100 37.000 33.000 3; 000 39000 36000 39 000 36000 34.000 34 000 37 000 lnta e 35.000 33000 33.000 34000 34 000 36 000 32.000 33000 4 000 35.000 Transform: Untranslormod 1-Talled l1otonlc Cone-% Mean N-Mcan Moan Min Max C\\1% N t.Stat Critical MSO Moan Control-1 28 400 0 9793 28 400 27.000 33.000 6 55 10 32.683 Comrol-2 29000 1 0000 29000 26.000 31.000 5 391 10 10 7 31 100 1.0724 31 00 29.000 33 000 5 129 10 -3017 2287 2.047 32 683 21,4 32.000 1 1034 2.000 30.000 36.000 6.421 10 -4 022 2.287 2 047 32683 42.8 33.500 1 1552 33 500 30.000 37 000 7 071 10

s. 8 2 287 2.047 32.683 es e 34.900 1 2034 34 900 31.000 39.000 5.957 10

-7 262 2287 2.047 32.683 ,oo 36.200 1 2483 6.200 33 000 39.000 5.646 10 ,8.715 2 287 2.047 32683 lnla e 33900 1 1690 33.900 32.00 36000 3 532 10 Auxdla!}'. Tuts Statistic Critical Skow K"lnlOgo vo est indicates normal d1stnbut,on (p > 0.01 0.87338 1.035 IJ.37827 Bartlett's Tes 1nd*cates equal variances (P ; 0.90) 1.590,49 15.0863 The control means are not srornflcanllk'. different !e = 0 '3! o eo11a 2 1u092 H)'.~thosls Test (1-tail. 0,05) NOEC LOEC ChV TU MSOa MSDp MSB MSE F-Prob Ounne1rs Te~t 100 >100 1 2 04668 0 07207 76 5367 4 00556 4 1 1 T~eatments vs Controt-1 Llnoar lnlorpolaUon (200 Resamplos) Point IC05 IC10 IC15 IC20 IC25 IC40 IC50 >1 00 >100 100 >100 100 >100 >100

S 40 35 C 30 0 g 25

~ 20 ~ IX 1S 10 5 0 ~ Page45 of94 so S? B 95%CL Skow 10 ~-----------~ 011 01! 07 oe 0 05 t 04 8_ 0 3 ¥l 02 IX O I 0.0 ----0---0--0 -0 1

O 2 0

04 -t-..--.~~-..-~~-.-...-~..--.~~ 0 50 100 o oso "I,, Oose-Rosponso Plot cD co ~ ¥1 1-ta,1 0.05 level or s1gm1,canee N,Moan 1.0000 1 0000 1 0000 1 0000 1 0000 1 0000 Kurt -00943 di 5, 54

T lnvt om,.ntal t01Un9 SolutfGni, fnc. Start Oat 5/15/2018 I quo rah uclear lant Outfall 101 - lntal< May 15-22, 2018 tatistical Analys Ceriodaphnia Survival and Reproduction Test-Reproduction Test JD* CdFRCR Sample tO: TVA/ SQN Intake End Date* 5/22/2018 Lab ID: ETS-Env1r Test ng Sol Sample Type* DMR-0,scharge Monitoring Report Semple Date May 2018 Protocol FWCHR-EPA-821 *R-02*013 Test Si;ec1e CO-Ceriodaphn1a dub,a Comments: Non-treated Cone-% 1 2 3 4 5 6 Control-1 28.000 28.000 27.000 29.000 33.000 28 000 Control-2 30.000 29.000 26.000 30.000 30.000 29.000 10.7 31.000 33.000 32.000 32.000 29.000 29.000 21 4 34.000 32.000 30.000 31.000 31.000 32.000 428 32.000 35.000 32.000 37.000 34 000 37 000 85.6 36.000 36.000 35,000 35 000 39.000 33 000 100 37 000 33 000 37 000 39000 36000 39.000 Intake 35 000 33 000 33.000 34 000 34 000 36.000 Transform: Untransformed Cone-% Mean N-Mean Mean Min Max Control-1 28.400 0.9793 28 400 27 000 33.000 Control-2 29 000 1.0000 29.000 26.000 31.000 10.7 31 100 1 0724 31.100 29.000 33.000 21.4 32.000 1.1034 32 000 30.000 36.000 42 8 33 500 1.1552 33.500 30.000 37.000 856 34.900 1.20.34 34.900 31.000 39.000 100 36.200 1.2483 36.200 33.000 39.000 Intake 33900 1 1690 33.900 32.000 36.000 Auxiliary Tests Shapiro-Wilk's Test 1nd1cates normal distnbution (p > 0 01) F-Test Indicates equal variances (p = 0.44 ) The control mean are not significantly different (p = 0.43) Hypothesis Test (1-tail, 0.05) Homoscedast1c t Test indicates no significant di erences Treatments vs Control-2 CV% 6.255 ~.391 5.129 6.421 7.071 5.957 5.646 3.532 7 27 000 31.000 33.000 30000 34.000 31.000 36.000 32.000 N 10 10 10 10 10 10 10 10 Statistic 0 94659 1 70543 0.80178 MSDu 1.07983 Dose-Respons Plot 45 40 35 c: 30 0., g 25 "C e 20 C. GI 0:: 15 10 5 0 ~ (',/ -e Q N ~ h u Page 46 of94 8 9 10 28.000 29.000 27.000 27.000 30.000 28.000 29.000 31.000 32.000 30.000 34.000 36.000 30 000 33.000 31 000 34.000 35.000 35 000 34.000 34.000 37 000 33.000 34 000 35 000 1-Talled t-Stat Cr tlcal MSD -7 869 1.734 1 080 Critical 0.868 6.54109 2.1 0092 MSDp MSB 0.03724 120.05 1-ta11, O 05 level of significance MSE 1.93889 Skew -0 4961 F-Prob 3.1E-07 Kurt -02125 df 1, 18

l l J pccies: Cerio({ap/111i11 tlubir, Client: TVA / Sequovah Nuclear P la nt, Outfall 101 D *1 Cl m V 1e1111stry: Da Page 6 of7 Date: 05-15-18 (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 I 2 Analyst H5 HS ,-t.J' t-1S ,._,,(' IA.) Concentration Parameter DH(S.U.l -:,,. p!(, f-* 9<,, "1-f3'7

J.92 1 -~'5

-~Aq DO (me/L) f."f e.o ?-71 -=,. fj '":{_ '8 'lei Conductivit (µmhos/cm) 31-z. 305

?oz CONTROL

Alknlinity

\\p\\ I..., C) (me CaCOJ/L)

Hnrdncss

~1- ~ g-..\\ ,, I (ma CaCOJ/L)

Temperature (*C).. '\\."\\

"\\S. \\ ""\\..~.b ~-0 "'\\.'-\\.., ""\\.S. \\ DH (S.U.l ""::/-'9S -=,..,-:, -=l,9(,, -:/.. '12 1-*Cf( 0.a'.) DO (mg/L) 8 -o -=,..q 1-B -:,.!J 't.~ 10.7% Contluetivity -Z.90 2£9'1 zq~ (µinhos/cm)

Ten1perature (oC)

"\\....\\. 8 "'\\...'\\.. k -i..-\\."'\\ "'-'l. k -u,\\. "'\\ ""\\.S. L oH (S.U.) '1-9ft> 7{.t:j{. "";/.9tJ f-*.,-z 7."J'Z l}_q ~ DO Cme/L) 8-1 --:,.. "t f?..o -=7, 6 =?-a q_t::) 21.4% Conductivity 2:ZS-1."1-8 ?.1-2 (11mhos/cm) "'Temperature (°C) "\\..U.-'\\ ~-0 "\\..'-\\. -""'\\ "\\.s. o ""\\ ~ *\\ \\S..O oH (S.U.) -f- '!(,, '"::1.9(p "":1.96 -:[. q, -=,.9.:3 -'l.ai:;i DO (mg/L) f?.,, f's.o P..c:, +-8 -:,. 9 /ll'.l. 42.8% Contlueti *ity 241 "2.4~ '2.37 (um hos/cm) "Temperature (oC) '\\.'-\\. '\\ \\."'* ( "'L~-\\ 1.-\\ -"\\ t..4-i "\\.'-\\,& pH (S.U.) "7--9"1 92 "1-96 7. ?J,q ":/.f!}q r.tM DO (mg/L) ~ - 1 8-a ~(

11. C/

Ef>.o (J.0 P:..G% Contluclivily /'::/-2 /":l(o /+I (111nhos/c111)

'fen1oernture (OC)

.. -;.o '\\.-\\. & "'\\.."'l-8 ~ \\.'\\ .,_ q. "1 -i'-'. ~ oH (S.U.) "'7-.9J.( -=l-9-z.. ~?5 '7.tUJ "':/-1'8 'l-t\\"1 DO (mg/L) '8.-z Pl. I

8. I

'(i./ £!.a A-A Conduclivily / 5& l"lt isa (111nhos/cm) 100% "Allmlinity s~ ~h (mg CaCOJ/l.)

llnrdncss (mg CaCOJ/L)

I.a& I. ""C) "TR chlorine (mg/L) <. 0.,o <o.m "Temper:iture (°C) '"\\.S.o "1.1.\\.,., i>\\-'\\ "1.S. \\ "\\.'-\\* t "1.'i -.f pH (S.U.l ,.-,,z. 7-.92 '"':f,'fS' ~-8B -=,.q( ~q4 DO (nu!IL) ~-'Z. ~., fl., 113-1 ff.o ~.Ot Contluctivil

tLf!1 1'19 (1tmhos/cm)

/52 100%

Alkalinity 5<..:,

S& lntnke (ma CaCOJ/L) 1,

Hnrdncss 1i,--\\

(ma CaCOJ/L) l9G "TR chlorine (mg/L) <0.10 c(..O, lO

Temperature (°C}

'-'l* & .,_~. i' t.'t -f -u\\*\\ '1.'-t. '\\ lnitinl Finni Initial Final initial Final Te111peratures pcrfom1ed at the time oftest ini1ialio11. renewal or tcnnination by the analyst identified in the Daily Renewal lnfommtion lnblc located on Page I Alkalinity, hardness nnd 101al residual chlorin pcrfomicd by the onalys1 idcittilicd on the bench sheet specific for cnch analysis and transcribed to thi bench sheet by: -+i.---* Page 47of94 SOP ATJ I - Exhibit ATI 1.2, revision 11 14

I J Page 7 of7 pecies: Ceriotlaplmia dubitt Client: T VA J Scouovah Nuclca1* Plant, Outfall ] 01 Date: 05-15-18 Day (Analyst identified for each day, pcrfonned pH, 0.0. und conductivity measurements only.) 3 4 5 Analyst tz..<., AA~ ~ rl.S rv Concen-Pllrnmeter ~ - tr:ition 1Jll (S.U.) .. lM 'i.ol ~.o-+ e,.o3 0.0~ 8 -o'f -:,..er, DO (m!!/L} ~-°' 'i-1.o ~.o ,.J,S ~.'--

'°1,+

'":/.-:/ Conductivity -3,.::;- 3~ 300 305 (umhos/cm) CONTR L

\\lk

llillil)'

~.,L \\..'I:'~ "'-~.. (mg CnCO;/Ll ' --~ \\.p\\

tb rdncss

&~ ~*, v~ (mg C:iCOJ/L)

Tcmper:iturc ("C)

'bt-1 "-S* \\ "'-~- i "\\S*\\ ,.<\\,& ._c;. '\\... "'\\.~-\\ nH (S.U.) 0.00 1.02- 'i.o3 e.111, A.a:+ 8..o~ 8-o\\ DO (mlZ/1.) f.l-.O, ~.o 'i,o ':\\-4 ~6 '"7i.fj -:z. ~ 10.7% Conductivity ~z.. ~g-o 2eo z~- (r1mhos/cm) "Temnernture (0C) \\.4,f "\\. '-\\. &- '-"\\-'\\ "'\\.S.o "\\."\\.-<\\ "l.S,0 "l..l.\\,! pH (S.U.) 1J.. no ~.01, %.c<+ 0.0'?) ~01" 8 -02. f?.o~ DO (mg/L} '.\\.Cl "R.&"l '1. £} ,~.'\\ ~-~ "':/..s_ ~e 21.4% Conduclivil}' ~62.. a1n ~7'0 (11mhos/cm} 2 7-Z "Tem perature ("C) ""\\A *.t l.c;..o "\\.)\\. '\\ ~-0 ... "\\.~ "'\\.S. O "-'-t. B o.H (S.U.) A,n ~.01, 'B.ocf 0.o\\ 8,01' 8.c,Z 8-cS DO (mg/L) 8.0 ~.o n,.ai>f ~ ii.Er t:\\..C\\ ~-+ -:i..a i-C/ 42.8% Comluctivlt * ~21-(11n1hos/cm) ~50 ~01" ZL/o

T emperature (°C) 1!{. ~

1..-\\- ~ 1.,.-{. '\\ "'\\S,.Q '1.1\\,'\\ \\.~."\\ l..L\\., oH (S.U.) 0bo ,..01 "+,Cfq (/J,/)0

env, ltf..oo B.03 D0 (111!!/L)

~ '";}.'\\ -:J-.Oi ~.o , -4, "'\\- i-B ':/.9 5.6% Contlucth*ily. {)q (11mhos/cm) l'lC\\ l,'b Pt/

T emperatu re (°C}

i...'-\\.,f l..'l, & 1..'\\. '\\ "'\\S-l.. i.t..\\.'\\ '"'\\5.. \\ ,._.... 4\\ 1>H (S.U.) ~.o I t',"ltp ~ t.>O '*\\..°'v 0.0~ 8.,:,o 8 -03 DO (me/Ll {:;,0

R 1',C\\

'\\{-i '-t-e, '"=1,g "':/-~ Conductivity t '54' lSS-tS\\ /!)~ (µmhos/c 111) JOO%

Allmlinity c; ~

(11111 CaCO;/L) ~Hnrdncss (mgCaCOJ/L) ~~ le"

TR chlorine (mg/L)

<..o. 10

T emperllture ("C)

\\.""*" l.~. \\ i.S, 0 .,_"i *" -i..s.o -UV\\ 1..q.4' uH(S.U.l ~u '1,-,q~ -::r

c:\\ I.,

0.02. 0-03 6'-.::il 8.03. DO (1112/L) t:S. 2. ":1.q -:i q ~}~ Hf -;f.t, E:,o Conductivity ~ \\'5'5" IW IS"t

lsc, (11mbos/cm) 100%

"Alkalinity Intake {mg CaCOJ/L} '5 & "Jinn.Ines ~o (mg CaCOJ/L) "TR chlorine (mg/L) <.o.1D

Temperature (°C) l.'"\\.* J

""\\.~ *f 1.s.o 'i.e.\\.* ~ 1..'-t

C\\

,s.o ~.o Initial Finni Initial Final Initial Finni Initial

Temperatures performed nt the Lune oftest in1tia11011. renewal or tcm1111at1011 by the analyst 1dc1111ficd,n the Daily Renewal lnrormation table located on Page I. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet s8 ccific for each ::malysis and transcribed lo this bench sheet by: --!1.--

Page 4 of 94 SOP ATI J - Exhibit ATI 1.2, revision 11-01-14 6 rlS 8-CH 71,R t..'\\. 4\\ 8...... c: "::(.t3 "\\."* R 8-o?. -=,.e "'1-*\\. f 80'3 -.:,. t? '\\'i,. \\ !J.oz "':{.13 ,A. k ~ -98 1 ,._':. *O 8-~ 8-o 1...-\\.E Final

E~vlronn e~lal Tesllng Solullon,, Inc. Con(entrutl n l'nn,metcr O:"'O lnlllnl '1111 (Slll 7 86 00 (m~/1.) 79 C.orulucfi\\ ii )' (1.unho~/cm) 312 ontrol

AJk11linit) <rnJl/l. C:iCO,l 6 1 llnrdncs, (mµ/L (11CO,)

81 Tcu111erature (C) 2~.7 inll 1Slll 795 OO(iui:/LI 80 10.7% Conduc11vi11* (11mho,/cml 2'>0 1 cm11crn1urc re, 1,1 8 lull (SUI 7.% ll.-1':I. DO (mi:/Ll ~ I Comluc1hih* l11111hu,/c1n) ~1* T~m,..,r,lturc 1'C) l<l.9 IDll 1Slil 7.96 -12 8"/., IJO (me/L) 8 I Conducth it,* (J,mllo,Jcm) ~4 1 r ~1111ker:11urc ('C) 24 9 1011 (Sl l 7.94 D01m!!IU 8.1 l<S.6% IComlocti\\it\\' (Junhu,/cm) In T<!1tti,cr:ilu1c ('<.') 13'(1 11111 /SU) 7 ?4 UO (me/Ll 8 2 IC011tlucti~i1, C11111hus/cm) ISG lflh% Alk11li11i1y (no)!II. C:iCO,J 58 I hu,Ines., lmi:/L C:oL01) lt8 1111~1 l{Miduul Chlorine (mi:/1.) 0 )0 !'c1111krnture t"C! 25 (\\ 11111 (SU) 7.92 OOt nn,/1.1 82 C11nd11c1h it1 lun,hu,Jcml 117 100*,1. lnta~* ,\\ll<ali11it) (1111:IL C:1CO,J 56 11:onlncs, (noi:/L CuCO,) llO Tu111l llc,id11:il Chlurinl' lme/1,l 010 l e 11111tn11urt 1'C) 2~.? TVA / cquoyah Nuclear Plant Outfall IOI Non-treated May 15-22 2018 Ceriml"pl111in tlubio Chronic Whole Ef0 11enl To, icit Tc t EPA-821-R-02-013 Mclho<.11002.0 0:111. hcmic:11 Anal ' C

Uoy I Oo,*2 l)n,, J Finni lt1i1i:1I Finul lnili11I l'ioul lnlti:tl l'in,11 7%

7 &~ 7.92 7 8.5 7 '}<} 7 99 8 01 so 77 7S 78 79 79 so 305 .,02 ,,s 60 81 l.i I 24.~ 1$0 J.I 7 25 I 2,11 25.1 797 7 90 7 9! 7 91 800 8 00 & 02 79 79 78 78 7 'l 7'J 80 ~8\\) 19-1 192 1-18 241 21 g 1n

15 l 1-18 218 7%

7 'lS 7 91 I *)1 1 ()<J 8 00 8 01 79 s l) n 7S 7.9 7.9 8.0 278 in !82

1) 0 147 1S 0 N7

-50 -"*8 1S.0 i 9(, 7 9S 7.9 1 7'H 7 Q<j ~00 80'! 80 80 711 7 'l 7 ') 80 RO 1M .!..,1 250 ?4 8 14 1 1 1.9 l H 2-IS 1,18 248 1 *n 7 9(, 7 89 711,9 7 98 8.00 801 80 8 I 79 80 s.o 79 79 176 111 17? 2U 248 1d () ?47 lH 148 24 8 1n 7 9S 1 88 788 7.93 801 7 9~ 8 I SI 8 I so 7,, 80 7'> 147 m 156 59 t,(l ii 10 24 7 14 IJ 25.1 lH

!H 119 15.1 7.92 7 95 u~

1 91 7.9 1 8.00 7 9S 8 I 8 I S I so 7.9 81 79 1~9 ,~2 I ~~ 58 (M 11 IU 1,1 k

!H 11 S 1~ 1 2.1?

JJ 8 14 8 11:1\\' ~ tnilinl 804 8.0 JOS 01 84 N8 S 01 l!U 180 14 9 8 0.1

71) 26'i 24 9.

8.o.l 7.9

!J7 1'1.9 7 99 7 I) 178 249 ll 00 79 155 58 b-1

, 0 10 .5.0 7% 1 9 157 S8 c,(l 0 10 1S 0 l'rujec1 nurnl er: ______ u_J_J7_ O~v S Un6 Finni lui1fol Fiori! lnitfal Finni S 03 H 03 &04 7 9? 8(1.J 78 76 77 7 7 78 300 lOS !5 1 148 !5 2 .n 14.9 8.03 !..07 8.04 ~ UI sos 7S 7,8 7S 78 7 8 180 ! &5 ~S.O 2~ Q J~.o !4 8 248 8 03 S 07 8.01 8.01 S 03 79 77 18 7S 7.8 170 H! 25 0 2H l.50 118 14.8 801 SJl7 80:! SOS 803 7 9 17 78 79 7S 217 '!40 lS 0 14 <J !~ Q 1-19 1S I 800 8 0(1 sou 8 03 S 02 li.O 77 78 7.9 7S 179 177 lS '2 24 O 15 I 1*1.'I 24 S 7 ')(, 110.J 8110 8 (ll 1 98 79 i.8 78 1 'I 79 ISi 15.t 1~ 9

!SO 24 9

!49

.!S 0 802 s.o 8 01 S 03 8 0~ 7.9 7.! H s.o 80 155 IS'1 2H H'i ~~ II !S.\\l 248

Page (oO Page __ , of_l __ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst I t Date analyzed O= l 5,~e; DPD: I CHM \\Cl.>"t Positive and Negative Control: Control Type Sample ID Result (..I) Positive Negative Negative control Deionized water Positive control Tap water Sample screening: Sample Sample ID Sample characteristics Result (,.I') number Positive Negative \\..!{,~ 11..\\. CTL Tv{\\ kn:,.~ \\()\\ no,,-i\\tr\\.. r,,,,.,, , t oe...,'-\\. -~ ~ \\~f\\l,(((_ I) c.<l-e +o.,, ru e,./ \\. ~ O~lS. 01-ct-)F\\e_Lt:> no w\\ C"\\ c J flf"t,,/ I lc"()'S,\\.~.0\\ /' 1-\\"-.Tf\\t.)()()~ O~h" (' l.OtJ. / I / V / / / ./'\\o/ o)Y / V / / / / / / Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A pos1tl\\le result indicates the presence of total residual chlorine above detection(> 0.10 mg/l}, which results 1n a pink to red color change with the addition of the DPD indicator. A negative r suit indicates the absence of total residual chlorine, which results in no color chan e with the addition of the DPD indicator. Page 50 of94 Reviewed by ~---.1!1,....,-----1 Date reviewed ~-c,"'-'5.._.-'-1=$--~'g"---~ SOP CB-Revision 4-Exhibit CB.l

Page _,.e.;:.;:...._ Page~/ ~ of__J____ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL= 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon M eter Analyst I T5 Iodide reagent : INR '6~ 9 Date analyzed O[.... /5 _ /8' Acid reagent: INR g 35 Calibration: 0.10 mg/L Reference standard # INSS 1.00 mg/L INSS /i /7 Slope (mV) (suggested ra!lge = 26 to 30 mV) 3 Note: For samples with a residual chlorine of > 1.0 mg/L, the samples mun be diluted to be within the calibration range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/l) (mg/L) INSS /~ /7 0.50 0,53 10 Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO = {(S - D) /[(S+D)/21} x 100 (mg/L) (acceptable range = :t 10%) I~ c,

(J""t-

-r..JP-.. SQ....> \\C> \\ 5

t. o.o 7 L

Duplicate D <'.:Q.01 Sample measurements: Sample Sample ID Total residual chloriru? number (mg/L) TV < O.OS mg/ L Method Blank (MB) L: (),() 1..2 TV = 0.30 mg/L Method Detection limit spike sample (MDLs) 0.3~0 \\ o<;.1'-l. o~ ,.J,... I ~r0 \\~Ile L. O.OQ.~ _/ / / / / / / V / ,<Y / ,,,,,./ ~ r----~ (j>Y '\\~ / ~ ~ ~ Note: All samples were analyzed in excess or EPA recommended holdin tome (15 minutes) unless otherwise noted. Laboratory control sample: INSS Reference standard number 7 Page 51 of94 True value (TV) (mg/L) 0.50 Measured value (MV) (mg/l)

0. 47Co Reviewed by '----K~ _ __.

% RS = MV / TV x 100 (acceptable range = 90 to 110%) o;o Date reviewed I C><;,\\~*lr SOP CB-Revision 4-Exhibit C8.2

I Page 62 Page _J_ of _J__ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Mat rix: Water, RL = 0.10 mg/L Analyst I -,-~ Date aoaly,ed C- /fi"_ DPD: I CHM IOOY Positrve and Negative Control: Control Type Sample ID Result (.I) Positive Negative Negative control Deionized water v Positive control Tap water V Sample screening: Sample Sample ID Sample characteristics Result (.I) number Positive Negative \\ f( 0~ II., 0~ -(..J,t,... \\~~ \\t) \\ no CO /o ( 1 C.fc. Q("' / \\e _.,_.,....,

~*.

\\, I no co lor I c/e,a r / \\ i crs.1 t.. as 11'1,l\\',(e__ I ( OS \\ '1. 01- £N~,e..\\...~ no co for, c lc.o.r ,/ \\&OS.,-,.Q\\ C..\\.\\ ~"P-.N~ /tfj'r,t- \\ft//Ou) J e, k_ar / - / / / / / / / A/\\y ,/ ~/;Y - r ~ / / / / ~ Nore: All samples were analyzed in excess of EPA recommended holdins ume (15 minutes) unless o herw1se noted. A positive result indicates the presence of total residual chlprine above detection (> 0.10 mg/l}, which results ma pink to red color change with the addition of the DPD indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator. Reviewed by I /) Date reviewed ~===O~S~-~l"'\\:_.-,... 6-:_-_~~-:_.-i Page 52 of94 SOP CB-Revision 4-Exhibit C8.l

Page 33 Page_/_ of _J_ Total Residual Chlorine {ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter: Accumet Model AB250 pH/mV/lon Meter Analyst I \\.S Date analyzed 05-17-1 c;r Calibration; 0.10 mg/L Reference standard # INSS /{p/f l.00 mg/L INSS /(iJ 17 Iodide reagent: I INR ~ 2 9 Acid reagent:. IN R ~35 Slope(mV) (suggested ran11e ~ 26 to 30 mV) .~n Note: for sample$ with a residual chlorine of > 1.0 mg/ the samples mu t be diluted to be within the caf1brat1on range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS : MV / TV x 100 number (mg/L) (mg/L) (acceptable range" 90 to 110%) INS$ /(i, t 7 0.50

0. 5?\\L.\\

/Oft, R 0/o Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO :: {(S - D) /[(S+D)/2)} x 100 (mg/L) (acceptable range= +/- 10%) \\k O'S. ll,.. o-.\\ ,v p._ \\ C,.Q t,.) \\C) \\ s L 0.0/}? l ' Duplicate D ~ 0.0/5 05-17-/'i 1.S - Sample measurements: Sample Samr,lolD Total residual chlorine number (mg/L) TV < 0.05 mg/l Method Blank (MB) .t. o.o,~ TV = 0.30 mg/L Method Detection limit spike sample (MDLs) 0.313 \\ k O~l'b.O~ \\°'J " \\ s (hJ I 1-JTA',(. ~ o.nn7 / / / _/ ./ _/ / \\C{.,/ ,, { \\Y NJ/ /v')~ ~ / /' ~ ~ Note: All gmples were analyzed in eJCcess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS : MV / TV x 100 number (mg/L) (mg/L) (acceptable rante" 90 to 110%) INS$ 1wn 0.50 (\\ SI~ 102..u 0/<) Reviewed by !._ __ _..A\\_,,_ _ ___, Dat reviewed j O ~

n-t&

Page 53 of94 SOP CB-Revision 4-Exhibit C8.2

I I I I I I I I Page (oL\\ Page _L of _J_ Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L Analyst l \\~ Date analy,ed 05- [- / f!;' oPo: I cHM 100 4 Positive and Negative Control: Control Type Sample ID Result{,/') Positive Negative Negative control Deionized water v Positive control Tap water v Sample screening: Sample Sample ID Sample characteristics Result (.I') number Positive Negative \\ It tt=...\\!-~ "\\" ~ 'Si:'i~ I()\\ no co /or I c. lc.'4 r I fQr-11 c\\ ~ ~ /

v

,~os. '<-o...\\ ~ tl-l~-.:..e no c:o /or > d e-Q. c- \\ k or:. \\ "- CS1.... Ft..}~,e..L~ heh.\\- +~\\ c./q.ud '-/ ./ \\ (OC:.\\<'\\-0 \\ ~ G\\p.,""t"t ""-~006-A. /ts'1+ '{ti/OW\\ C/ood'( v / / / / / / / ,\\~/ ~JY o/ ,VI/ y V / _/ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A positive result indicates the presence of total residual chlorine above detection(> 0.10 mg/l), which results in a pink to red color change with the addition of the OPO indicator. A negative result indicates the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator ~ FA.L~e. P~\\'t\\l..le. f-o.t. -r, t/1\\ ('S~~ SM'\\.P,e. A:,;5 \\ b\\.e,es, ltJ ttJ!LFetl...AX.£.. Reviewed by l1---J1:1-l-t------1 (J'---- Date reviewed~. __ Cl~S-*_1~'\\-*I_J _ _ Page 54 of94 SOP CB-Revision 4-Exhibit C8.l

I I I I I I I I I I I I I I I Page 4 Page~l~of __j__ Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, Rl = 0.10 rng/L, Meter: Accurnet Model AB250 pH/mV/lon Meter Analyst I \\"""::, Iodide reagent: I INR '6;Jj Date analyzed Oo-1'1-L ~ Acid reagent:. INR ~35 Calibration: Slope (mV) 0.10 mg/L 1.00 mg/L (suggested range = 26 to 30 mV) Reference standard It INSS /(; /7 INSS /G, I 7 d~ Note: For samples with a residual chlorine of > 1.0 mg/L, Lhe samples must be diluted to be w thin the calibrauon range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS :: MV / TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) INSS /(, /7 0.50 0,5/../J.. /(Jg J./ O/o Duplicate sample precision: Sample number Sample ID . Total residual chlorine YoRPD : {(S

D) /[(S+D)/21} x 100 (mg/L)

(acceptable range = :t 10%) \\ s ()!,. \\ C.* (f',. 1-nJf\\\\ ~Q.~ \\C)\\ s L 0.01.;z 1-Duplicate D LO.0/Q n.:-_1q-/~ Sample measurements: Sample Sample ID Total residual chlorine number (mg/L) TV< 0.05 mg/L Method Blank (MB) <O QJ~I TV = 0.30 mg/L Method Detection Limit spike sample (MDLs)

o. 3:; l Uo<;.\\i_.()'-\\

'°T'J~\\ S.Q.~,~e_ t:. (). 004 ./ / / / ~ /"' _/ n ~ ,(J-' ( D_/ ,...,.,.,,y {JJ/ / --\\ ;::,,Y' / /"' / / ~ Note: All samples were analyzed in excess of EPA recommended holding um (15 minutes) unless otherwise noted Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/l) (mg/L) (acceptable range = 90 to 110%) INSS /Co17 0.50 c). 5J,..,:.,, /06.0 °lo Reviewed by.... I --~'-)'{...,.__........ Date reviewed I o S *1 ~-lc;f Page 55 of94 SOP CB-Revision 4-Exhibit C8.2

j l Page -z..o Page l of~~- Alkalinity (SM 2320 B-2011) Analyst /1-... &sc.::.L-.. ____ ~J Date analyzed C>~*l"2.,,'& J Matrix: Wat r, Rl = 5.0 mg CaCQ3/L, Samples are titrated to pH :: 4.5 s.u Titrant normality and multiplier determination: ntrant reftrenu Normality check numb r 5 nd rd number INR "o!>ID INS$ lv~' Laboratory control standard (LCS): Reference ndard Tru value (TV) number (mg CaCO,/L) ( ss \\\\,~t> 100 Duplicate sample precision: S mple Sample ID Duplicate (D) ample mea urements* Begin ml o., Sample volume (ml) 100 Sample olume (ml) so j Sample number Sample ID End Total ml ml (E) 1:u, i~.s Be in End m ml ,~.l,p J~~ 0 t*n End Ill Ja. .V\\.l. Sample volume (mll MB (TV< 2.S mg/l) Oeion zed water, pH = 5'.~\\I $U 100 l OS, \\O,t-e A I J O'S. Dti* J8 QA. f.\\-l Dm (2... Oo A l. DM<Z.. ~A-c. Otnfl-A-eU-Dn,fl..- {Y)(oA-<.- DfrV2... rnec.n...

Dma, La

control standard (LCS}:

Reference st.lnd:ird numb r INSS Page 56 of94 r !u....L~TV~li--..:;;Sa::.:.;m~p=le (mg C CO.J/l) volume""" (ml) 100 100 B gin 100 5"0 lo-o \\ I End ml R vrewed by: Nofmarty (N) of HiSO* pH Factor or Multiplier = (S ml N zCOJ x O.OS)/ = O.ZS/E

(N x 50000)/ 100 ml sample (acceptable range e 0.0180 - 0.0220)

>> N>tSOO Total ml ,. 'l Total ml Begin ml o.o 70, '1. ~" I,, -:19.. C\\ .14.~ ~ o.o 1o.o );!,. \\ l"a.1.. ,!l,5 a*ts D,O ~.% iof+ '.2.1.1, o'l-.q O.b \\0$ ~~.C> 31..i Total ml

0. l)~OO Multlplier 10,1)

Mui 'plier End ml 0.1 ~Q.I,, 3Y..~ Lf-.1 ;). .1-\\S.$ t,.u ,~.\\ ,,g,; ~g.~ 3<t~ 4-"'t. '6' 9.i Zo.'t 31.1, 3'"T.9 .~ 10.l> ~.::i.... 2..l.i "B,C:, Multiplier /0,t) Alkafinity (MV) %RS: MV/TVxlOO (mg CaC03/L) ( cceptablerange = 90 lo 110%) , _ _ (.qq_:9 °I 'L c~lc. Alkalinity (m,: CaCO.J/lJ

,.0°1.

os 61< <<... dS*,ug Total Multipli r Alk.lllnity (ma CaCO,/l) ml 0. 1 \\O;O j,O )."'i I'+ ~.; 3.~ 6,:!> l,.,o l,. J t,:i.. 1.#2. J1:1.2.. 100 11.~ I\\O )(),0 100 ~.i lo Ir I I t:> h.l-t,.~ ~., \\0,1> ,,. 'l. \\U"\\ 10.6 l\\O l\\. \\ - Alkalinity (MV) (mg caC0-3/l) % RS : MV /TV 100 (ao:eptahl r n e: 90 to UO%) Date reviewed: ( 05,BIB SOP C - e 1s10n 4-Exhibi C6.l

Pag Page 2-of__._ _ Alkalinity {SM 2320 B-2011) Analyst l &4S'- Date analy2ed US*\\'l,*,?, Matriic: Water, R : s.o mg CaCQ3/L, sample!. are rated to pH= 4 5 S.U. Titrant normality and multiplier determination: 8 in End m m INR INSS laboratory control tandard (LCS): R ference stand rd T ue value (1v) 5 mple Begin tnd number (mg CaC01/l) volume ml ml (mL) INSS \\\\o3~ 100 100 o.c 9.,! Duplicate sample precision: S mple Sampe Sample ID volume Begin End number (ml) ml ml \\ R 6~o'\\,\\& U"r6h1*~~ Q,~ '\\$ 14~ t, Duplicate (DJ l- !-+.~ 16.. '1 Sample measurements: Sample Snmple number Sample ID volume(ml) Mti, V' - .... -~- ,,orf W:ttPr nH : \\ @. c:,510. 0 ~ t h.n m ho. 01...a

,,5

, loSoi.1.0 "-r../A 5~ Prtn 5o , ~oso2. *u AtniNt- \\le.=,'12,\\& Pih t&oc;a.f.\\" flt\\ ir-Jr -- l Jc.OSo&. o\\ rf\\l A BF I\\.\\ I 5o ,e oso~. ')J I 1... \\ \\.~O~\\\\.\\" j .3 ..L , tosoe.O"\\.- rfJA-f>f N 1>JT I ~ \\ &'OS 0"\\, 1.~ I z I \\ E-<>~ \\ \\. l 4\\ J 3 J.-. \\ ~ n~ 4f1.IC> C°fl.J.-mar\\ I ~."5 , ~ o~o°'. i..-i.. I '2. I ,. roS \\\\. \\t .1 3 J- \\C: DScn.. o"'\\ ~+-tn~r\\ <<w t ..1-S \\ k 0~ 0 °'* °1c'!> I

i.

I \\< O<;\\\\. \\ °'- l J ..J- \\kOScn.,~ ~~hn&v\\ Anh ' RS \\ ~OSo'\\, l-"L- ~ 2- _L Laboratory control standard {LCS}: Refer nced.,.ndard True.valu (TV) Sample llecin End number (me---.. ~,

-***-e ml ml (ml)

INSS 100 100 Reviewed by Page57of94 Total ml C\\.~ Tot I ml ~ I,. 'i,S 8egon ml ,s.q ?£\\,Q .,3c;,S' 2."I.'-" M., ?,~~ 04.\\ ~ ~.\\p 0.1> ~-1 Ul,? 9.S II,It, \\-\\.q l\\eS \\'ii4 .2.10-+ .:l~.:1-o\\--4>~ Total ml Multipll r Alkalinity (MV) (mgC... o,/L) \\o.o C,"t, Alkallnlly Multipr.e, (mg CaC03/L) s pH F ctor or Mui lph (IV l\\ soooo)/ 100 ml mpl *

Nx 500
MV/TVXlOO (accept.a I t"ilng = 90 to l lOW.)

9S.t> 0 /p %RPO

. ((S - O) /l(S+D)/2)) lOO (acceptable ranC"e : r 10%)

10.0,.. \\ l~o D )O;<> L*I) )i-o tl,<;1., -b..5*12.*l\\' End Total Multiplier Alkallnitv ml ml (mg CaCOJ/l) 10:0 ~ ~<;;*\\'2'\\\\ "7,.._q ?_,O >'-) ~~~ ....,,...... ~ ~S.'S'" --+:~ .,. \\ 91-. 29.1., '°4,1

i,\\

~ O"'+,l 4-5 ')_) ~Q 2.~.4..,. _;, I-,,, g\\p '+I.~ ~., 1'1'.'\\ V2- ~I,..

i..,

.. ~ lo '2- '-1-~.C\\ 3.~ .'2., L,lp 8.1 31 ~, lo-Z. 1#,3 ~.2. .., \\ L,\\+ '1.5 3 2. .')'\\ lf't 1.1. \\,,

1 I I u.'

'&+ 13,,'I ~.2, 4.L) ql.. '"'5

l "' I i..)

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14)

-:,1.,

lit.j ~.; 14) --

'h Mul plier I allni y (MVI MV/TV JOO (mg CaCO,/LJ I ccepi.ab range 110") B'>t. S, 1'2.**8 ate r vi wed* L.:O:..'S::.:..:.17:::>:J.>G---' SOP 6-Rev,sion 4-Exh bit C6.1

Page 1-""2. C Page 3 of_4-___ _ C: :-- _),..-.,... fnljloof; ~ Alkalinity (SM 2320 B-2011) Analyst I E,'51,.., oate analyzed OS',\\"2.A'zS Matrix: Water, RL = 5.0 mg CaC03/L, Samples are titrated to pH= 4.5 S.U. Tftrant normality and multiplier determination-Titt~-M _Normalitv check Begin fnd Total number standard number ml ml (E) INR INSS Laboratory control standard {LCS}: Reference standard True value (TV) Sample Begin End number (mg CaCOJ/L) volum ml ml (ml) INSS 11.c2,"3 100 100 2.tp,1 2J~.~ Duplicate sample precision: Sample Sample Sample ID volume Begin End number Ao H (ml) ml ml . \\ & <:Ast mu""' 6

i_5: l.

Duplicate (D) J_ 2ft*,o 1+1~ Sr:mple measurements: Sample Sample number Sample 10 volume (ml) MR 1n, - ~ r __ :onirea **- L -- .... U - ~- -, '-' o<. o-\\. o'-\\ Q:rs+<<-tC("' Anh (lw l 2.5 , ~ osoci.. 1.~ I I &0S 1 I. 1"-. j oS * <r:.

lk' A ml-\\sw fh'\\};

I ,:,~ - t.fb *i& 6 l 2, (\\~* c.:,i. \\ ~ J 3 I f 'O':, Oi, O\\ ~-Pi CV I&. oSO<\\* 1.\\ I 2- \\KOS.\\\\, \\S .1 2 ,.e-oSl\\k.ol.. l\\o Wnt; ha<<" ' I f OS 10. crz_ I ?- \\ ~eiSl1.. O\\ l 3 , kri:;of. o"1 i?)rc.lJ.P t \\ "0S10.G3 I

i I K"0'::.11.. <J1...

J .3 ,,~o~.oR (_;,("\\f'.. o..l htnd I \\kr,<.10. !)~ .JI 2 18.~r,-~ J .3 \\ $.s:OS<>~,O'\\ PAsQ111 ~"'"'- ~ Laboratory control standard (LCS}: INSS Page 58 of94 True value (TV) Sample (mg CaCOJ 100 (ml) 100

2.

6 Begin ml I.!- }"00 I.L Joe> Q, 5-c ~ 'I ~LL I .L

2.

I \\ End ml Reviewed by: Normality (N) of HzSO, pH Factor or Multiplier - f~ ml NazCOJ X 0.05)/E = O.ZS/E = (N x 50000)/ 100 ml sample (accepta~" n,so. 0.0220) =N X 500 - -e:s.... 't)C5. \\'2.., i Total Multiplier Alkalinlty (MV) %RS= MV/ TV><l00 ml (mg CaCOJ/l) (acceptable range = 90 to 110%) 9.'9 loo 'll.# q~.oO/p Alkalinlty %RPO Total Multiplier fmg CaC0;/1.) = {(S - 0) /((S+D)/ZJ} x 100 ml (acceptable range= :t 10%) s ~.s lo.a 100

1.

D .:?.S l.:>O ~ --i)°S,l'L**.'g ~ Beg:n ml '+\\.'5 ~&.S '-4-6.ic 0,0 lq, \\ \\;\\. I 18.'1.. Z..1.:1* ~1.11:g ~).2,.. &~.1 3if,o lf~.i o.~ J.+.& 9-~ \\S'.< ..i21. "I ~g.; Total ml tnd Total Multiplier Alkalinity ml ml (mg CaCOJ/L) S<ir,. - O'S*1l*\\ '+S.S ~.O I Ll\\ tO:O <jC -4:5',b

J.1

( ln 8'4- '4-"1 :'l ~. ~ I ~ "bB. lc,, I lo.I l.Dl ~-1 v,.O Lq:.::, I~.?- "- *I UZI ?~-.'1- "+ s J.!15 .?~.'e, '+.I "f\\ 01.-i. ~* 4-4 Y-,,'2.- 2..o I."?.) ~o ~re~ ~.'&

1..)

91o 4.<.? 4.'2? ?_, ci.... 4<<-o 5.-i /'7..o) )O'C> t) '+~ .i...1-~ l 1...o} q,,,o °i.3 '{,S- ~) q.siO IS.$" t., :i f.....,, ~I ()'C ~ 1.<I l,U. I C'"o) ?,t,oe> ,qll.'- C,,4-( C"'O'I .;-z.oo ~Vs 4,,o r -1....J o!o*O t'"o ) Muitlplier Alkalinity (MV) (mg CaCOJ/l) % RS=MV /TVx 100 (acceptable range '°90 to 110%) Date reviewed: I 05*~16 J SOP C6-RP.vi~inn 4-FYhihit (.fi.1 i

.) ~ilJ.,;,;~; k Page '23 Page ~ - of 4: Allcalinity (SM 2320 B-2011) Analyst I BSL Date analyzed i---o=S"'-.,-2.-,-, 'B- - ---i Matrix: Water, RL = 5.0 mg CaC01/L, Samples are titrated to pH :: 4.5 S.U. Titrant normality and multiplier determination: Titr;,nt-ri,f,.rence Normality check Begin End Total Normality (N) of H:SO~ pH Factor or Multiplier number stanaaru,.u, ml ml - (S ml Na:C01 x O.OS)/ E c: 0.2S/E = (N x 50000)/ 100 ml sample (CJ ~-.,cee-* - L -~--- =- 0.0180 - 0.0220)

NxSOO INR INSS Se,._

O:,*IL.. *1 5c. Laboratory control standard (LCS)* Refe rence sta ndard True value (TV) sample Begin End Total Multii,lier Alkalinity (MV) % RS: MV / TVX 100 number (mg CaC0 1/L) volume ml ml ITll (mg CaC01/ l} (acceptable range = 90 to 110%) (ml) INSS \\\\0'3(3 100 100 ai.-; 4\\.'\\ cu,, lo.o C!ID.O C\\ ie.C,:,/o Duplicate sample precision: Sample Alkalinity %RPO Sample Sample ID volume Begin End To!al Multiplier (me caCOJ/Ll {(S - D) /l(S+0)/2]} x 100 number (ml) ml ml ml (acce ptable range =.t 10%) \\~<61~. GS ~o. s.c:, l..O t().rJA; 2. s C2,o:>O j A'AA8 i.. o.o 3,9 3.4 I co) lo.O l L 0 C "Z()Oc>> \\. i-> Duplicate (D) '.. 3.4 +.,g 3.~ I yo) 10.0 P..Si- -t:.S('?.,/ Sample measurements: Sample Begin End Total Multiplier Alkalinlly Sample number Sample 10 volume (ml) ml ml ml {mg CaCOJ/l) MB (TV <..2." ~.'~, -. * : __... YYOL'il; J, t- ___ s.u. JUU EK.,<., ~-,z.,f. ' ~ t,<;,,.

c:i4

.Pa~ w k,,1<.. 3 2-l.<l \\"U) ~,_ ' 1rb) IC,o 2,1c t:> l(OS.c,L\\0 c. ~tlc...h ( Sl c; 1'2-C> 15,; 3.~ I 1.1.\\ l.~o \\/( (610,[)lo (

2.

I \\'5..; I'~.; .2. 0 .1-1) l'w> IX'()':,\\'"l *OS \\ ...3 ..1-16.~ 2,'0. cy ol.~ I q ) 100 P,* Mil (>.... \\ Ji ~S6k, I\\ I

5

?,.o.'l ~4.'ii ~.'I ! ~) tt:,o \\ ~ (1:110

Ui

{

2.

I <<4,j ~ -'+ ~"' l-4) I /.In l 8 oS 1"1..o\\o j ..2, ....L ..,78.4 ~. _2,. ~ I i4,) .H-'7.<:.~ 06-11.. 1-BJl. Sa..us w lo<' -~.. i+'l. \\ o lo.<;'" ,. ~G ,~*-(1oo) 05.\\'LlRi o~ { J_ ~ ~ >. =.q 'l.~ --- -~~ 05 1'M(j ~ J / / V ( Bi;t.. ~-- - -. *o Laboratory control standard (LCS}: Referente standard True value {TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS = MV / TV K 100 number (mg CaCOJ/L) volume ml ml ml (mg caco,/l) (acceptal>le range " 90 to UOY.) (ml) INSS \\ll13'1 100 100 62.., ~ .lo q,-:r 10.0 q-=r cn.E:P\\'o Reviewed by: iiC Date reviewed: b5.\\.3,~(j ] Page 59 of94 SOP C6-Revision 4-Exhihit C6. l

Pag Pag _ _.__of~-- --~---- Analyst I : Date an lyzed ~ ~ .......;:O~*-'-:::i ... *..:..1.=;..._ __ --J Alkalinity (SM 2320 B-2011) Matrix. W ter, RL = 5.0 tng CaCO /L, mples are t,tr ted to pH = 4.5 S.U. T;tront normality and multiplier determination: Titrant r f rence Normality checl.. Begin nd Total ormality (/11) of HtSO* pH Fact11ror Multlpll r number stand rd nu_mber ml ml ml

(5 ml Na2C01 IC 0.05)/ = 0.25/E

" (/II x SOOOO)/ 100 ml sampl {El (acceptable range.,. 0.0180

0.0220)

=/111< soo INR 'i3<, INS5 ILto+ 0.l ,~.\\, l~S'" 0.020'0 ) t),O I Laboratory control standard (LCS}: Ref renc standard rue value (TV) Sampl B gin End Total Multiph r Alkallnity (MV) "RS: MV /TV x 100 number (me CaC01/l) volume ml ml m l (mg C;iCOJ/L) (acceptab e range = 90 to 1.10") (ml) l INSS \\"3! 100 100 ,~.(p ~.5 'f :'i \\0,0 9 -, Gf 'l o 0 /o Dup cote somp e prec s,on: II f

S mple lkalinity

%RPO amp! Sample 10 volume Be.gin End Total Multiptl r (mgC CO t/L) "'((5

OJ /[(S+0)/2]} x l QO number (ml) ml ml ml

{acceptab.le range = t 10 %} 06.\\\\o.\\6e:, m1+slJ..J 100 ~~-.; ~I.P L,.1 10.0 s '4, I ..l Duplicate (OJ .J.- .J_ 0 ~~.'- 3-+,".} t,.1 ~ I ~L. - o5,1Cf,,1 Sample measurements: mpl ee In Eod Total Mui *pr r Alkal nitv Sample oumbllr Sample ID volume (m l) ml ml ml (mg CaC01/L) M B (TV < 2.5 ma/W Deionized wat, pH : '5.lol. s_u 100 o.o o., O.J 10.0 J.o OS-16.6 ~ O'tl~ aq.. "l 4o.w S."i 5"f 0S, l5.1Af.:> I '"'°*" ~:5 5A '5q 1'{)'6,. \\\\o. \\6 A-l P,*O-(4'.o 16,o ld-::, ~-* \\l cP.:>'1...'.o\\ Cd. CL um~ lt;,,o I.:\\. I t..,,t t.z I ~ J 0 '7 (fl

Drna,

\\;l, I I 'R iJ. v?. ~ :t. ,a.(,. 11.)*\\i f\\ 1\\1 ">W vV \\ \\ lf.'t- ~'4.'+ l, :;:) <.,o o S* II.-\\C I\\ I

z.

~~.3.?. 2, 5",q S'\\ nC.-11,d i & J 3 ~ .2-o,-'J

i,, * * (p u.2>

"~ \\i uS 1'-\\. C:>1-H'"'lf.,4-S&..'f'-) I 0\\ I 5'o BJ.,,l.l ~s ..:;>.'i I 7."\\ Sl' It OS 11... C>~ I 2-31.5" ~.'+ ..,.4 I 'J ) 5"'i ,.t~,,.~ ~ .3 i+a.'+- ~. ~ ~ q { 1) <"l \\t os1~l-tn. , IJ A SQ~ ov ' '45.; &f'b:2.. ~~ ( 'l.) 5'1 \\Pl"!< \\ b. ctl I 2.. o.o ..?:1- .d."i-( ?. ) ~ \\xoS 1~ - ~ .l 2 ~ 5.'i .3.} P - 1 l,'2. \\i C,.:,. I '-4 * {fli.. -f #,-5&.tJ j III l \\ 5.'i ~.... -1~ (,.) SIP L ~ n, I lo c:i<. I z ~-Ip 11.c; ~.q ( V\\ 5~ '"O<;. \\ {c. t>-.{ .l 3 I l.S 14.~ ~.tl L l.) Sq \\ &' o<; l~. o~ -rJA. 5&-.) f N\\ VI.I \\ l'+.'t-I°'.};?. ,;},<:/. (.: ~) ~(,,, 2-I£ IY.:.. l b-()(,, J. l ~.1 2,, :0 o\\.i I 2.\\ 5 <. Laboratory control standard (LCS}: Ref renc standard True. value I ' "I Beain End Total M11ltip i r Alka 111 y (MV) %RS= MV /TV lOO number (m CaC01/L) volume ml m<. (mg QC0 1/ ll (acceptable range "90 10 110%) (ml) I SS 100 100 &s1. 05.f"\\,1'5 Revi w d by*

c D te r vi w d. j05. \\Cj.~

Page 60 of94 OP C6-R 1sion 4-E hlb1l C6.1

I I I I I I 1: Page -25 Page a? of __...;;,.___ Alkalinity (SM 2320 B-2011) Analyst I :(, Date analyzed 1--=-- --~---~ L.. _.:;.x...*..:..19.i...,.* 1.::.. IJ __ Matrix: Water, RL = 5.0 mg CaCQ3/L, Samples are titrated to pH = 4.5 S.U. Titrant normality and multiplier determination: Tltrant r "'~rmalitv check Begin End Total Normality (N) of H2SO* pH Factor or Multiplier number standard number ml ml ... ~ I C ml Na,co, x 0.05)/E - 0.25/E " (N x 50000)/ 100 ml sa mple (£) (acceptable range= o. .- -,01 = N x 500 INR INSS f;61., OS°ol C/,j'g Laboratory control standard (LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS = MV / Tl/ X 100 number (mg caC03/l) volume ml ml ml (mg CaC01/ l) (acceptable range= 90 to 110%) (ml) INSS H,3Z 100 100 ~a.o c<'n q,-:r 10,0 en. Cf:}'l>(o Duplicate sample precision: Sample Alkalinity %RPO Sample Sam ple ID volume Begin End Total Multiplier (mg CaCOJ/ l) " {(S - D) /[(S+D)/2]} x 100 number (h\\)~ (ml) t'nl ml ml (acceptable range =.t 10%) a~wos\\c~ S} 3;>>.S", l'1, 1i Sample measurements. Sample Begin End Total Multiplier Alkalinity Sample number Sample ID volume (ml) ml ml ml (mg CaCOJ/l) MB 1n1- ~ ~ - - "iieo water, pH = 5.U. 100 8!,(. O'S,I <f *It £1:;f. \\I. OQ6\\U ml); IOO ~'1.'l

2,<g 9
\\.0 lo.o j,::, j ~I\\ mOmL l!i .~.., ~ :+ -~.'& I~'\\ l~O \\ ~n::.. \\£..CM. --fy'A~N l1....\\i uv -3 ~.,{ ~-~.;s.~ .::i ~ t :,_\\ S"'h e, "C:.,c;. ~ <;, ku d I \\~ n o 0.5 o.S 5.o Ix(.)<;,,. o-z.-. \\ l,. \\ o.'!: -~'T 1.9 lq ........ t: \\.k oS IC..
crz_.
3 j.... ..2.'+ .~'l, 1.4-I'-+ \\ " Cb,s.o \\ ~u.l-JccnOO Ii 0,.... l 2..5 .3 :'3 ~.o '5. l- ~\\ ? 10 \\ &o<;,,.c\\ I
i.
<=t,Q l'"t.~ t:;.2, ->, ) '2. 10 l ~<JS1G\\, o\\ j 3 J_ \\4-.~ l~~.4 ~-1 I t.I ) ,;/"4-0 I/ [.,.-/' ~ ,,,/" ~ / ( C':)t.. 05¥1-1~ Laboratory control standard (LCS): Reference standard True value (TV) Sample Be11in nd Total Multiplier Alkalinity (MV) % RS "MV / TV x 100 number (mg CaC0 3/ l) volume ml ml ml (mg cacO,/l) (acceptable range =- 90 to 110%) (ml) INSS 1<. 51 100 100 ~o.+ ~ ."C) <\\.\\o )o,O C\\l,, q <<c..<::> ~ I':) Reviewed by: Date reviewed: I Q5.tPi. \\.6 Page 61 of94 <;OP rF.-RPvi~inn 4-Fxhihit r.n.1
I Analyst ~-~ =x.....----- --1 Oat analyzed ~-t>_S_,_l'2-_ *l_'S ___ ~ ritrunt normality and mu/tlplier determination: lltr.,nt referanc Normality c.h c:I< 8 in End t1umber standard number ml ml INR '&t>~ INSS IS'D'S o.o 10.t> Laboratory control standard {LCS): Re f r nee st, rtdard lf'IJI! value {TV) S mple 8 gin number (mgcaco /L) volume ml {ml) INS 1(,, '~ 40 50 10.0 Duplicate sqmple precision: Sample Sample Sample l volume B gn number (mt) ml S. Ptf'll t'Jt S'o !7... 1 Total ml (E) \\ 0,0 nd ml I~,\\ £nd ml \\3.'l fr.i3... Page ~l_v _ Page __...___of :3 Hardness (SM 2340 C-2011) Matrix: Water, RL"' 5.0 mg CaCOl/L Normarll (N) of EDT,<<\\ pH ~c<<iror Multipl
0.2/
"' (N x soooo)f so ml nmple (acceptable rnnge = 0.0180
0.0220)
Nx 1000 0.0.2.0 0 1,o. o Total Mull,plier Hardness (MV)
% RS:MV /TV xlOO ml (mg CICOJ/l) ( a:eptable range = 90 to 110") 4,1 t.o.o -'+2. 105. bQ/~ Hardn ss %RPO Total Multiplier (mg caco,/l)
((S
D) /((S+0)/21} 11100 ml (acceptable range = t 10" )
l.'1 s ro.o 3'+ Duplicate (0) _I 11' '4.11 .L 0 ,~.'l> 15'.S 1.":> 3a+ &51... -OS,1'2A'l Sample mea urements: Sample 8 in nd Totlll Multipl er Hardr.~ Sample number Sample ID volume (ml) ml ml ml (mg CaCOJ/lJ MB llV < 2.5 rng/l) Deioni.ted water so 0,C) o.o o.o 1-o.o tvD S. Pln.t,,n I IS.5 I Ip.I, I. I
1 :l.
Oo,o-=r-ie, SSUJ I 1'6.S 11,,1, I. 4 ~~ ()f..If, 16 .1 UL5 ~o.5 7..,0 'iO os.o'.'.}, H!J fV\\l~SW ~o.':> ~--f.,'1- "+ '2. 'i'\\- OS.,o..ieA I R_4.':t- ~.b ""1.\\ 'i1.. os.1o~~ei lb J -, 2..'11.'3 ~... 1'1......... 2- 'ii+,
J" ~.-
- -. AC l~C'\\~~. C \\ c.d ftl Orn<< ?,?,. 0 ~'l.'l.. lt,1-Sa+ ,L PP A-l. [)r(\\(L 3t.2.. ~ "+ ,;.. '2. i-t-I& C>SH:>,Oi rrumhMH '"h.4 4,15.C> "'. te, 130 I 051)18 ~ I \\ ILJfl \\ R. o c. 0 ~~1.0 1v'Ast+~....i A-rn 45 O.o o,>.~ ,~}:;) q 5,~~.,1..,i rzo ~ , kosoe-1..\\ fun it0t d.t\\ 5.~ .J.v St 100 ~ I \\(r.<..l'\\~-\\ t Pm 5.~ ~.4- ~., ~ 11.0
l 1 tosot. \\C\\
Pn1 l ~T ,.1 11;0 ~*" ii-'l.. \\00 ~, \\ Soso ~.~\\ -.,JA !?JFN ,,,?C::, 11.0 .~.j \\,~ b._ 1'd. IR o~o"."1.o,.\\ I z I \\~.'B I &.I-.~ l.v l.4- \\ k OS\\ \\ * \\& j 3 11+4 ,~.\\ L 1-t8 \\ ~ oso8.o'l..- 1' JA eFr.J, t-.)1 \\ 2~ ,~., 1":\\.~ I.L. 1.4 \\ ~ o'5.o'\\.1.,S I 2 j \\"1.'1-',..,~- 1.'l k" l aboratory control standard {LCS): Reference ndaro '1'/'tl~lue (TV) Sample B in fnd Total Multplier Hardn ss % RS = MV /lVx 100 number (mg CaC01/l) II ml ml mgCaCO / l) (aa:eptable rang :s90to UO%) "~ (ml) r-- INSS 40 so ML z-,.,,rz.. t-RevlC!wed by: Date reviewed: I 05. \\ Z-16 Page 62 of94 SOP CG*R vision 4-Exhiblt C6.l
I I I I I I ( I ' I Analyst ~...::es::.c.-<.,------1* Date analyzed 1:>6.n... l ~ Titrant normality and multiplier determinaUon: Tit
eference Normality check Begin End Total number
.Stan"u:u1,,1.. ~.. *-- ml ml (El JNR INSS Laboratory control standard {LCS): Reference standard True value (TV) Sample Begin End number (mgCaCOJ/l) volum ml ml (ml) INSS I~\\~ 40 50 ,~.+
1,,2, Duplicate sample precision:
Sample Sample Sample ID volume Begin End number (ml) ml ml \\K.OS l \\,\\ '\\ rfv A & F.iv ll\\ll3 .;z5 ..11.; ~5.o J, Duplicate (DI .L o<.!,o c,1'4-.'=l-Sample measurements: Sample Sample number Sample ID volume(ml) MB1",., L. *.,...,.,*~, ~ia-:...... ~..... _.. "Ir c:n \\R,~.rn. <J?, PR~tm~ \\ ~5 , rOScA.~'- I
2.
1&-os 1\\.lf J 3 \\ ~oScrt.o...\\ ~ HnClf'\\ "w J , ie. oSc'\\* "~ I z ,i-oS.\\\\,I'\\ ~ 3 cS.-~\\~ f\\ In 1-lsvJ fn.m l '5"'C oS'*~*\\k e, I J. I os-o,*d~* J ?; _L ~ lO s. <Si
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l:::Asl--muA Anh I ~5' I ~c,SQ\\.1..1-I 2 14 os.11.1x J 3 ,.k uscr,.o<t I-~trru:.ri l1 ufttih' I , k o~,....::. 1.:i. I z l~OSl\\., \\'1 I 3 - \\ f CJS<n.o \\ 5f>iN.. l .~n I~ OS.O°I * ;..\\ I
2.
l~O~\\\\. \\'5 J .3 , ko~oJ:. olo ~t It 1' N'Y'ltvn I _L-- L b t d d( J a ora orv contra stan ar LCS: Reference standard True value I'., - ,Im Bel!in End number (mg CaC03/L) volume ml (ml) INSS 40 50 Reviewed by: Page 63 of94 2 Page"""--~'--- Page ,I' of 3 aS-c,5.1;i.,1'it Hardness (SM 2340 C-2011) Matrix: Water, RL:::: 5.0 mg CaC03/L Normality (N} of EDTA pH Factor or Multiplier - 0.2/E "'(N x 50000)/ SO ml sample la ""' *~* - n nl80
0.021.0}
=NxlOOO -ss.. 1:>S.\\2,r"c. Total Multiplier Hardness (MV) % RS = MV /TV x 100 ml (mg CaC0.1/l) (acceptable range = 90 to 110%} 1.9 'JQ.o 2>3 'iS.~ 01,.., Hardness %RPO Total Multiplier (mg CaC01/L) ,. {(S
D} /[(S+0)/21) x 100 ml (accepiable ranee * :t 10%)
t.'1 s vi ,1,, 1..0.0 I '"1' j_. D l.'1 li6 eF.,l, - oS.1'2... i Begin End Total Multiplier Hardness ml ml ml (mg CaC01/L) &,... 0<;;,11.ii o<'-J..'t .;[~.o ~.~ I :2.., 1.,0,0 "'2.. .2":}.o .2 q.5 .:).s Joo o?9.S 3l.5 3.o 17-Q 3.i..s.~.,, '~ '1Q ';u..L l, '2.1~.a ?,t::> _'.i:.L_ ____
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J.'O 100
't.o 11.i .Q,?, 110 I I* '6 13-1" I q '1-lo \\ ;,-:i, IS.'6 .2, I 'i4- ,s.i. 1'5,1
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~'".\\-.~ ..;.i 1\\p Total Mullipfier Hardness % RS=MV /TVxlOO (mg CaCOJ/l) (acceptable range,,,. 90 to 110%) -~l. O:S,1'2 *18 Date reviewed: ! ~-\\2,..l.(3 SOP CG-Revision 4-Exhlbit C6.1
I I I I I I I I I I I
r;::i C""? -
11_ Analyst J S5L-Date analyzed 1-t:> ....:~c..:..::.,::...2,._.-, q-----i Page I'S Page 3 of... ~'--~ Hardness (SM 2340 C-2011} Matrix: Water, RL = 5.0 mg CaC03/l Titrant normality and multiplier determination: ntrant reference Normality check Begin ~,... T.~, Normality (N) of EOTA pH Factor or Multiplier - ------..*~.9.:!JE number standard number ml ml ml = (N x SOOOO)/ 50 ml sample (E) (acceptable range : 0,U.J.!Slf
u,u ~
=N>< 1000 INR INSS BSt. b ~.,:i *\\1S Laboratory control standard {LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Hardness (MV) % RS=MV /TV X 100 number (mg CaC03/l) volume ml ml ml (mg caC03/ll (acceptable range= 90 to 110%) (ml) INSS It. I~ 40 50 ~ ** :i, ~q.~
i.o
-i>>.o .i+o 10'0.d:l/o Duplicate sample precision: Sample Hardness %RPO Sample Samp e,- volume Beg1n End Total Multiplier (mg CaC03/L) = {(S
0) /[(S+D)/2)),c 100 number (ml) - r--mt--~ ml ml (acceptable range = +/- 10%)
---r--- s Duplicate (D) ~lE\\2.~ Sample measurements: Sample Begin End Total Multiplier Hardness Sample number Sample ID volume (ml) ml ml ml (mg CaCOJ/l} ,,_~~ 1-. .,..., C 0!:,,\\-:2 *\\ \\ e~u
u*
1.k oS, o, ~'1.- 'R.)U\\.V'l'\\ h cun 2. °'"' ~,.; 3~.'i i.\\o "1.J).O 1-::i-t> I &' OC:..1'2
0 \\
J 3 J.. n.-, ~.3 'g,4 I I~ \\.fo,,'IA.. l~ 1~n1*N-.hn 6~.Cf I ~c:;
c:: Sl
,':>,,\\ 1..,'1---- 1"2-0 I"\\. ) ./ ./ V /"" ,,/' ,,,,/ / / / / ( \\...._ ~ A<..., c:6,f'l*li Laboratory control standard {LCS): Reference standard True value (TV) Sample Begin End Total Multiplier Hardness % RS=MV /TV x 100 number (mg CaC03/L) volume ml ml ml (mg CaCOJ/l) (acceptable range = 90 to 110%) (ml) 1N$S lv I 'g 40 so 3~.9 "1-o.'l .;?,() "t,l:>.O Y,b \\~.o0 to Reviewed by: Date reviewed: !Q6./'l.,f (') Page 64 of94 SOP CG-Revision 4-Exhiblt C6.l
Analyst I &sv Date analyzed b5,1q *l'T> Titrant normality and mu/tip/fer determination: Titrant reference Normality check Begin End Total number standard number ml ml ml (E) INR '&~ INSS,s~~ o.c 10.0 10.c I Laboratory control standard {LCS}: Reference standard True value (TV) Sample Begin End number (mg CaC01/L) volume ml ml (ml) INSS \\\\,1\\-g 40 50 10.0 \\'20 Duplicate sample precision: Sample Sample Sample ID volume Begin End number (ml) ml ml os.,1,,,,1e6 VY\\~W So 12.0 It,,'2,.. ~ Duplicate (D) J- '""* '2-Jo a+- Sample measurements: Sample Sample number Sample ID volume (ml) MB (TV< 2.5 mg/LJ DeionizC!d water 50 06.1e1~1t mlt-SW oE; 1.A.1 ea .J ()C;. J L, \\-8 A-(hj+1,.lAJ I IC b~i.""\\. o\\ (l(i(~ l:)tY'l(l.., ~ Dn r..vt brf"\\'2.- I ,...c. -,o-\\i' A "" 1-\\ c...u u v I t)~ ~\\lo*\\i fl\\ I 2 Q~
l'I.* \\.~ ~
J .3 \\A u~1'-l. lS'2-1.J/Jt. ~t)~ lo\\ l ~'5 I Sc. () C:, \\\\ "',.l \\ ,z. \\ ll: "~\\ f. u-:, .3 1 c. oS 1'-\\. <rL 11/A SlS\\11 \\1,' I \\ ~ OS\\ lo..t>...\\ I 2 1.R 0~ \\~.a,... l 3 ,~re:::.,...t.~ "l../ /),, 6&\\..) lt.l T" l \\ &. O'S 1 \\o. Cf) I
2.
\\ R. t\\C \\ l2. ()'-( I 3 \\ ls I)"::, *'-l-Cl') -QA -S~t-l t ~vu I I i ~ll..()~ l 2, Latmcotory control standard {LCS}: Reference standaro- -'fro&4'.I TV) Sample Begin End number (mg caco1/l} vo....... _ ml ml (ml) INSS 40 so Reviewed by: Page 65 of94 Page _!~ -- Page - ~ -of 2. Hardness (SM 2340 C-2011) Matrix: Water, Rl = 5.0 mg CaCO /l Normality (NJ of EDTA pH Factor or Multiplier =0.2/E = (N x 50000)/ 50 ml sample (acceptable range = 0.0180
0.0220)
"' NX lOOO 0.0~t>O 1-<=>,0 Total Multiplier Hardness (MV) % RS= MV / TV x 100 ml (mg CaC01/l) (acceptable range = 90 to U.0%) .2..o 1.Q.o i;,o.P< a.:: )00. ()~k, Hardness %RPO Total Multiplier (mg CaCO,/l) = {(S - D) /[(5+0)/21} x 100 ml (acceptable range = +/- 10%) ~:2.. 1-o:o s <iJaf .L 0 .14. t.- ~a+ ef:,1.. -- b$,1Cj,tf Begin End Total MUitipiier Hardness ml ml ml (mg CaC01/L) Z.~.o 0,C> 0.() O.o ~-- NO ..!>n.<+ .21+.1,, 4.2. ~ I"\\,\\\\ '5li~ ~~-"' d'! i ~ -'2. ~4 ~~ -2:
u 0
>-\\-.1.. ~4- ~:a...o 3'1,1 J.4..1 _j2. .31.1 ,4.J_~ '+*'2. ~1+ 4-1.~ ~ LI-4.\\ 2'2. ~5.4-J.+q *"' ,4., '2. '84 o.o '-+.L A-t.1.... '1i4 "+, '2.. fi.q I.~ L,& 5."I °1'1-l. I< ... 1 ls,o 1".4-C!.o u,
'7 \\
~4 't.o 10.'S 1:S ( :2 \\ L,,O t,:;,.S l~.I ),\\p I "2) t.i-i. l..l I l:~."l l.1.t "2. l l.4-l~.':t 15'. "2.. 1.45" _'"\\..,s l,o ,s., I t.,,i" I. IP 2.f" L,&J. 111,q lK:t \\.S ... \\ lto I 'I.:l, 1q.i 1.5 11'7 ') lDo l'U ~I,? ,.c:; ~)-..... l-o Total Multiplier Hardness % RS" MV /TV x 100 ml (mg caco1/l) (acceptable rang = 90 to 110%) ~ 0 $ l'l -l'x Dale reviewed: !05.l"H0 SOP CG-Revision 4-Exhibit C6.l
I I Analyst I '£1,'6c.. Date analyzed===o:s:.:,"l::.i=~======== Titrant normality ond multiplier determination: Titrani reference Normality check Begin End Total number stanoaro num--, ml (E) INR INSS Laboratory control standard {LCS): Reference standard True value (TV) Sample Begln End number (mg CaC01/l) volume ml ml (ml) INSS He\\"S 40 50 ~,.~ ~3.3 Duplicate sample precision: Sample Sample Sample ID volume Begin End number 3 (ml) ml ml \\A: c6l8.o--l tYA-SC~ I~ uv ~5 .:>~.:2. l.:>..i.i ~ Duplicate (D) .l- ~4."b .JI.,.~ Sample measurements: Sample Sample number Sample ID volume (mLJ mo \\IV <.<.:, l)'lg/l) ue1on1zeo walt, C'f'> ,&. l)~\\c;. - U"'L- £nke.J cl l 5o \\kO~\\ "\\ * (rL. I
2.
I \\ (:-OS,)'\\-cn- ~ .3 _L... \\ k K,S. C>\\ Otu.!lct~~ ' o7S ,£rt:. l-:J,{)\\. I 2 I lf<:610\\.0\\ J -6 _L ../"' ~ ~ ~ / Laboratory control standard {LCS): Referenc standard True value (TV) Sample Begin End number (mg cacoi/L) volume ml ml (ml) INSS H.i<~ 40 so '-t-3.o '-fS.o Reviewed by: Page 66 of94 Page '2-o Page ~ of__,,:a-_ Hardness (SM 2340 C-2011) Matrix: Water, RL = 5.0 mg Ca(Q3/L Normality {N) of EDTA pH Factor or Multiplier = 0.2/E = {N x 50000)/ SO ml sample 1a -~ - 0.0180
0.0220)
= N X 1000 ---a.:.. -.IQ.IQ Total Multiplier Hardness (MV) % RS = MV /TV JI 100 ml (mg CaC01/l) (acceptable range"' 90 to 110%) ~ - 0 1,.:,,o ~ 1o0.n°b Hardness %RPO Total Multiplier (mg CaC01/L) = {(S
OJ /((S+D)/21} x 100
, ml (acceptable range =+/- 10%) I _.c; t i-u"lo,o s t...o ~,..L D I.S l ~o esl.. -os.,~.,, Begin End Total Multiplier Hardness ml ml ml (mg CaCO,/l) ~ os.,~.\\~
?t,,3

l')-.~
0.9 'ZJO,O Ii .:n. '). <<'S.'+ , * '2., .:)&.+ fl*), - -~- _ 31.o \\.S ,~o .;2,z;,4 33, 0 ~-"',.,_, I c;( n ob/Q 31'-"lr "+.'+ .2. ') l'Ko 3"1,o '+3.o '-1-.o ( :i. )--- I l.:>'C> / / Qa t)~1JCl,l'il Total Multiplier Hardness % RS= MV / TV x 100 ml (mg CaC01/L) (acceptable range = 90 to 110") ,Jn 1,,o.o ~ lot> n °/n Date reviewed: I ();, I'\\, w' SOP CG-Revision 4-Exhibit C6.1
Page 67 of94 'equ yah uclear Plant Biomonit ring M l - 22. _QJ Appendix D Referenc o *icant e t and ontrol hart
U'\\ N u ro "'O I " Pimephales promelas EnvlfonmtotaJ J.Jtln9 SoH.ntons. Inc. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.2 1.0 0.8 0.6 0.4 1.2 1.0 0.8 0.6 0.4 Control Limits(+/- 2 Standard Deviations) --.-~.-----.=--.--.~*~---.--*-~.~-.--.--**-----.--.-
Warning Limit s
-**~--........ ____,____.----~=---------*--.;..---4*-~----------*----------.:'.'---~* Test date 7-day IC25 = 25% inhibition concentration. An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti-logarithmic values) Control Limits (mean logarithmic 1<;5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic 1Cz5 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- SA.75 converted to anti-logarithmic values. SA.75 = 751h percentile of CVs reported nationally by USEPA) Page 68 of94
i CD E*1i111011mtft1, 1 ft'IIIOtJ lohHlont. kt ~ Pimephaf es prome/as Chronic Reference Toxicant Control Chart Source: In-house Culture \\,I Log10 Conversion Anti-logarithmic Values (c/L KCI) Ti t number Test date 7-day IC25 Laboratory Calculated CV ToxCal Determination 7-day ICu CT 5 CT Control Limits warning Limits (g/L KCI) CT
lS CT + lS CT - 2CV CT + 2CV 1
12-06-16 0.8303 -0.0808 -0.1423 00433 0.7206 0 5904 0.8795 0.5399 0.9411 2 01-10-17 0.7066 -0.1508 -0 1433 0.0432 0 7189 0.5892 0.8773 0.5384 0 9392 3 02-07-17 0.7052 -0.1517 -0.1421 0.0426 0.7209 0.5924 0.8772 0.5426 0 9378 4 03-07-17 0.7620 -0.1181 -0.1362 0.0365 0.7308 0.6178 0.8646 0 5761 0.9139 5 04-04-17 0.7043 -0.1523
0.1392 0 0351 0.7258 0.6174 0 8531 0.5765 0.9012 6
05-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 O.S434 0.5939 0,8858 7 OS-16-17 0.6906 0.1608 -0.1382 0.0320 0.7275 0.6278 0.8431 0.5904 08864 8 06-06-17 0.7367 -0.1327" -0.1393 0.0314 0.7256 0.6280 0 8384 0.5911 0.8810 9 07-11-17 0.7519 -0.1238 -0.1393 0.0314 0.7257 0.6280 0.8385 0.5911 0.8811 10 08-08-17 0.6829 0.1656 -0 1379 0.0294 0.7280 0.6357 0.8336 0.6012 0.8731 11 09-12-17 0.7271 O.l 38'1
0.1371 0.0292 0.7294 0.6377 0.8342 0.6037 0.8730 12 10-03-17 0.7024
-0.1534 -0.1382 0.0293 0.7274 0.6355 0.8326 0.6011 0.8720 13 11-07-17 0.6666 0.176]
0.1'121 0.0289 0.7210 0.6310 0.8238 0.5961 0.8636 14 U-07-17 0.6847 0.1645
-0.1404 0.0265 0.7237 0 6406 0.8175 0.6089 0.8533 15 12-05-17 0.7191 -0.1432
0.1424 0.0251 0.7205 0.6418 0.8089 0.6112 0.8432 16 01-09-18 0.7574
-0.1207 -0.1397 0.0244 0.7249 0.6478 0.8113 0.6185 0.844] 17 02-06-18 0.7951 -0.0996 0.1377 0.0260 0.7283 0.6461 0.8211 0.6154 0 8557 18 03-06-18 0.7002 -0.1548 -0.1387 0.0263 0 7266 0 6437 0.8201 0.6126 0.8552 19 04-03-18 0.6973 -0.1566 -0.1411 0.0256 07225 0.6423 0.8128 0.6115 0.8474 20 05-08-18 0.7204 -0.1424 -0.1429 0.0243 0 7196 0.6435 0.8048 0 6138 0.8380 Not/:!: 7-diy ICu ~ 2S"l. 111h1b1tion concenv atlon An estlrnatloo of The potassium t hlor,de concentrallon thal would cause a 25?. reduction In P,meplloles growth (calculated using ToXCalc) CT = Central tend!!>ncy of the IC,, values Control Llm,ts = Mean loganthmlt IC1, +/- 2 standard deviations converted to anto-logauthm,c v.1lues Warning Limits = Mean logarithmic ICu +/- 2CV or s,.,s converted to antHoganthmlc values. Sus= Standard dev,a11on corre~pondin@,o the 7S'" percentlle of CVs report.eel nationally by USEPA (SAP-
O 38)
C\\I "' Coert,clent of 11<1**at1on 75th Percentile CV waming Limits CT. SA.75 CT +SA]~ 0.4468 1.0009 0.4457 0.9921 0.4469 0.9948 0.4531 1.0086 0.4500 1.0015 0.4521 1.0063 0.4511 1.0040 0.4499 10013 0.4499 1.0014 0.4513 10046 0 4522 1.0065 0 4510 1.0038 0.4470 0.9949 0.4487 0.9987 0.4467 0.9943 0.4495 1.0004 0.4516 1.0051 0.4505 1.0027 0.4480 0.9971 0.4462 0.9931
.s:::. ~ 0.... CJ 0........ C: 0 u 0 ~ C 0 *.:: Ill "i: ro > - 0.... C ClJ *a
E ClJ 0 u 0
0 - 0 V') ~
a.
1.25 QI 1.00 CV > ro 0.75 ro :~ C: 0.50 QI) E 0.25 20 .c ~ 0... (!) 0.... 10 C 0 u... 0 - 0 30 20 10 Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture
---- ----*- --'!*:...----. ---* -------.::*:.--=--------
__._. ~- - USE PA Minimum Accep ance Criteria (> 0.25 mg per surviving larvae) USEPA Maximum Coefficient of Variation Guidance Criteria(< 20%) ~-- --- -
-----*-4****-----------,.--.----------~.=---1-~.
._~ _ __.._.......__.....__~--~-~~--J---1-_~-~-~~--~-~-~~-........ ---__. USEPA Upper PMSD Bound Acceptance Criteria (< 30%) USEPA Lower PMSD Bound (>12%) Control Growth, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sens1t1v1ty of the test method and is not a minimum acceptance criteria. Central Tendency (mean Control Growth, 0/ or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations)
i: Control Pimephales promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Determination Control Growth Control Growth CV Control Growth Test (mi/1M1il IIIVH) (") Test number Test date Survival Mean CV MSD PMSD CT 95% Confidence Interval CT 95% Confidence Interval 11'1 (mg/1n,tiai luv**I (!,o) 12-06-16 100 0.617 5.6 O.U67 2 01-10-17 100 0.587 7.3 0.0629 3 02-07-17 100 0.579 lLS 0.07Sl 4 03-07-17 100 0.638 6.2 0.0662 5 04-04-17 100 0.620 s.s 0.0919 6 05-02-17 100 0.729 S.6 0.0792 7 05-16-17 JOO 0.593 2.8 0.0521 8 06-06-17 100 0.608 7,2 0.0542 9 07-11-17 100 0.713 3 0.0589 10 08-08-17 100 0.638 7.9 0.0791 l 09-12-17 100 0.631 S.6 0.0562 12 10-03-17 100 0.658 4.4 0.0838 13 11-07-17 100 0.738 3.9 0.0641 14 11-07-17 100 0.727 0.8 0.0 80 15 11-0S-1-7 100 0.676 2..3 0-0607 16 01-09-18 100 0.6U 8..3 O.O?S 17 02-06-18 100 0.772 8,4 0.0115 1 03*06-18 100 0.810 4.7 0.0640 19 04-03-18 100 0,743 6.1 0.0836 20 05-08-.18 100 0.576 11.9 0.0516 Note~ Cof'trol Sur.Iva! w USEP m1n1m1..11n tut a<<,pc b1111vot1tt1o1 g.~ 1u4v.,vt t C()ntrol Me:an Growth -: VSE.P~ rmn,murn tell I c*ptab111tv crner1a ~ O 5 mc/su'Vi f'! 1 rwe CV:. co,mciiml o II r, oon rorconuot aro\\vth USE.PA m.m\\Jm Cl/ u,dance. cottna f oercentt1e c. 2 MSO =- Mff'\\1"lum, .fan d1fftt nc~ PMSO.a P *fttnt mintmum ** n1ftant d1fTtU!nt.e (1'1 CT - 25 CT-t25 CT-ZS CH2S 18.9 0599 0413 0786 5 7 0.0 11.4 10.7 0.600 0414 0 7 6 57 0.0 11 3 13.0 0.591 0.420 0 763 62 0.3 120 10.4 0.590 0421 0759 62 0.4 12 0 14.8 0.5 l 0.422 761 6.3 0.6 11 9 10.9 0 596 0 416 0776 6.2 0.6 118 8.8 0.603 0.434 0.771 6.0 02 lL8 8.9 0,601 0 434 0 768 5.8 0.7 10.8 8.3 0.609 0 43 0.781 56 0.5 10 7 12.4 0.616 0 451 0781 59 LO 10.8 8.9 0626 0 489 0.764 5.9 10 10 8 12.7 0637 0525 0.748 60 I 2 10.7 8.7 0.645 0 52 0.761 57 l I 10.n 8.Q 0.642 0 535 0.749
s.
.6 10.0 9.0 0.647 0545 0.750 5.2 0.4 10.0 12..3 0.643 543 0.7 4 54 05 10.4 10.6 0.651 0 S3 0.765 56 05 t0.7 7.9 0660 0.526 0.794 56 05 10.7 11.3 0.668 0 S 4 0.802 s 0 10.'7 9.0 0.663 0.523 0.803 5,6 07 10.S P SO ls a me;nure of ten pr b.siOn Th PMSO s tht "'"-" De-rceN ddf,,nee tt~twe1:.n I controt nd tr atm,ot tf..1t un t>e decl eQ.s.ta11srlca y J n1f+c.a"t t w ,~,h11.tent to 1atv tru Lowor PMSO boo"4 ltrm>nN bv u;EP 110' ~*c*n, l > IN ft lowtr PM'iO bound t~ 1e_se,nu pra r to tne stn111,viw o* tht test n,_ t'tod rld,i not a mtnimun,
1pDn cri, r,a Uoper P1v1SD bound *<< p11*1<* u "'"' dot rm,nea by U!.EPI\\ 190' ptlttnt*if, < 30'!(
CT= C*~tr I t ndtnc-, of lhc 11ow1h, CV at PMSO **' -; St.1ndard di:oY,*tlan of,he g,owth V or J:.M~ lloilue Test PMSD 11'1 CT 95% Confidence Interval CT-25 CT* ZS 11.2 4.6 l7 8 11 2 4.6 17 7 11.4 4.8 17 9 11.2 4.7 177 11.3. 4.7 17.9 11.d 5.0 17.8 11 4 5.0 17.8 11.0 5.2 16 8 10.8 49 16 8 11.0 S.1 16.9 10.8 5.0 16 S 11.0 5.4 16 6 10.8 5.2 16 4 10.S s.o 16 l 10,6 5.0 16 l 10.8 5.0 16 10.6 5.0 16 2 106 4.9 16.3 10.7 5.1 16 3 10.8 S.3 163
Page I of6 £11110:,nm,~uJ Ttillnq Soludo,u, tl'lc. Pota sium hloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimepha/es promelas PpKCICR Test Number: 39 DU11tio11 nrenm*ntion informntio11: Commeuts: KCI Stock rNSS number: INSS \\lo(S Stock preparation: 50 g KCI/L: Dissolve 50 a KCI in 1-L Milli*Q water. Dilulion prep (mg/L) JOO 4::iO 600 750 900 1050 Stock volume (ml) 9 13.5 18 22.5 27 3I
Diluent volume (ml) 1491 1486.5 1482
]477.5 1473 1468.- Total volume (m~) l::iOO 1500 1500 1500 1500 l:iOO Test onmnism i11formnlio11: Test informntio11: Or!?.anism source: In-house culture Randomizing template: \\..\\~ (\\ u,..,l(,. Age: < 24-hours old Incubator number and 1_& shelf location: Spawn dale: 0~
O'\\..-\\&
Artemia CHM number: CHM984 Hat~h dares and times: OS-;),*\\ 'ti: \\~~'C) Dry!11g i11for111atio11 for weight -rb os-o.f- \\~ 0~00 deter111i11ntio11: Transfer vessel infonnation: pH =,.go s.u. Date / Time in oven: ~-\\S*lt ms<; Temperature = '"I.\\.S oc Initial oven temperature: lot>. (. Average transfer volume: < 0.25 mL Date/ Time out of oven: O':>*l\\.*\\4 cnss Final oven temperature: bO. C.. Total drying time: 1..'-\\- ~o..:i.tS Daily feeding mul reuewnf information: Day D:itr:
t.fornini;: feeding Afternoon feeding Test initiation, i*enew;,I, MHSW or tcrminnlion batch u ed T ime Annlyst Time Analyst Time Analyst 0
05-08-18 oc; \\c; v\\ \\\\LS ,ll o,:iS x\\ OS*~**e i\\ ( 05-09-18 t>l.:,n o \\,f \\ £..00 ,11 0£00 11 OS*cr'l..*IJ h 2 05-10-18 \\J - "i 1"11.-.r\\O y( \\L,00 \\t 0 ~QC) os-o ~- \\ i' f, 3 ~ u* 05-1 1-18 Q\\c,OC) \\1.00 ,I{ O~<Y) At N;.-CJl* *K & 4 OS.- 12-! 8 O\\o~ O \\{ \\.1..~ 0 w l')A ~t. ll{ I\\<:.* rt"\\-\\ of-5 05-13-18 Olo~O ~ l "1. "\\.(\\ o~~o l,( M,-<n-1} 6 05-1 4-18 ~ Ql... 00 k\\ \\ "1..0C) O&bb l"l,-cn-lR 7 05-15-18 \\J o, 0\\)~S, R Co11trol illformatio11: Acccptnncc critcrin S1111111w1J1 of test emlooi11ts: % Mortalitv: D1, s:20% 7-day LCso 85&. '1 Average weight per initial larvae: o. S l L NOEC looo Average weight per surviving larvae: u.s,6 ~ 0.2:5 mg/lan*nc LOEC 1<:. O ChV lo 10- g IC2s ,1.0-11 Page 72 of94 SOP AT2l-ExhibitAT21.I, revision 11 14
l Page 2 of6 Species: Pimephales promelas PpKCICR Test Number: 39 Survival n11d Growth Data Day Control 300 mg KCI/L 450 m~ KCI/L A B C D E F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 JO JO l to It) 10 10 IO (t) co IO ! \\) { () ID ID 2 / 0 10 l t) l C) 10 I \\.J l\\) tO l 1) IQ 10 lO 3 £ t) IQ \\0 It) IO I<:) l C) tu 10 (C) tO ( i) 4 /0 l t) It:> 10 /C) fC) to lb I \\:) LC) ID LC 5 /0 IC) 10 10 CQ LC) l C) ( \\.'.:) (0 l C) ( \\) 10 6 to IQ I O ro It) I t) < a lD l C) (C) LC) {() 7 /0 , C) (\\) 10 'C) 10 ' t) (~ ( \\) I{) I () LO A ~ Pnn wcigllt (mg} Trny color code:; '..furc;rr 8CUO 14.'?3 15.~t?. /{ 91 15.3~ 15.45 15.N Jw.51 JtO~ 15.(lo 15.J~ 15.~J.,~.Cf I Anni *sl:
fS Date: Q4 * :;t£1-I If:
D = l'nn + Lanae weight (mg) J\\. a5 ~O.*'f1 o< \\. ;i~ Annlysr: TS ~C.4-5 J.0.75 Jf,O~ ~.all J.J..19 l3.l.3 ~.(j5 al.39 cl.I.~.? '.;:: le:..!l5:.=..J:1-j ~ C
Lnrvnc weight (mg) = 8 -,\\
llnml cnlculnted. s.1o'l. s.s~ I.,. n S.1 \\ "'-1~ i.:~o 1.o.S'\\ 1.,.'\\~ $.1~ I..,\\'\\ S. foS <,,.~:i Annlyst: ~ Weight per initial number of larvae (mg) "' C / Initial number of larvae I~ q..."? rv ~/>'} "°' 0 A.4; ~
4) linnd calcnlnlcd.
~ '>:) Y} ~ ~ ~ '\\II \\() \\.D 'D ~ ' 0 o* 0 Cf o* er Average weight per initial Pcrccnl number of larvae (ow) rctluclion
o. 'S,i\\,

o. lol,A
- \\~-~,. Cl. S<\\ &
~- & 7.
from conlrol (%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm u unusually mall, lg= unusually large, d&r = decanted and returned, w - wounded. Comment: Page 73 of94 SOP AT21 - Exhibit AT21.I, revision 11-01 -14
I
1
. j J Page 3 of6 _. £n.v1ranmtntlt l'f!11Uli9Sokrt on\\ lnc. pecie : Pimeplta/e5 promelas PpKCICR Test Number: 39 Survival a11d Growth Data Day 600m1 KCI/L 750 mg KCI/L 900 m: K CI/L M N 0 p Q R s T u V w X 0 10 10 10 10 10 JO 10 10 10 10 10 10 I to I Q / IJ ID I ti IO ID 10 & zrA.. C\\ '~ °' '"' g~ 2 r o I 0 l () lD ID q1 A. IQ I () g '\\ t\\. 8 3 10 t C) 1 t> l Q 10 &.,\\ I() ~D 1'~ q q g 4 /0 l C) I l) 10 '\\J g 10 l C) \\ r'" tA ~ 5 IQ ,A. u! ,<( ~ ~J.. IQ { C:::, le) &>\\ ~ G\\ t\\ 8 lo\\. .u( ,-,z ,c.l ~\\... \\J.. 5u\\. 6 °I'>. ID / () 10 fs 8 °' s s 7 ,a 10,o q 'l i &,t.( '{.~ 'i l{ *c:\\ *.f" A= Pnn weight (mg) Trny color code:: £acec:,-\\: ~ gll.!) /4.wO 15. 'l3 fll. ~~ 16.JI 15.SCo /5.<?4 15.~0 J5.G2 15.J.5 14. 7.3 15.05 15. J(j Anolyst: TS Dntc: Ql\\
21~ -18:
(J = Pnn + L:1rvnc weigh! (mg) ~.01 I?, 17 Anni *sl: IS ~0.70 3.1.50 /1.0. (pJ. J.O. ?i l<l,7q ao,3q 19. <t~ 11.io \\1.Sq IR JL.\\ Gnrc: a.5::- 1::Z-1~ C
Lnrvnc weight (mg) "' 8 -
llnml cnlc11l:1ml. 1,,_10 S. \\o'"l '-* ~\\:, 5.~, l.f, \\~ ~."IS 4,\\:t:\\'-\\ *V~o... er.,,.,-.\\1,.s'\\ "2. s.-t 1\\11 11lyst: e~ Wei hi per lnili~l numbcr of lnrvne (mg) / lnitinl number of lnrvnc A (>.~ J,.." ':,;). '-,~ .t, ob ~o ~'\\!' ~~ llnn!I cnlculnl d. cf "'.)
i-I\\,
\\g '6 I\\, '\\; "v ~ o* Annlyst: o* 0 o* 0. 0 o* 0 o* o* Cf ()° A vcrngc weigh I per initial Percent num ber of lnrvnc (mg) rctluctiou
0. 'S'\\~
-:,.01. fro m control
0. \\.\\~'\\
"2.\\.l.'1 7.
o. l.S1...
S.lo* ~ 1. (%) Commei1t codes: c = clear, d = dead, fg = fungus k = killed, rn... missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 74 of94 SOP AT21 - Exhibit AT21.1, revision I J-01-14
l ~ P C-:J - I _ EmJtonnmu,11 felting Sohn kins. lnc. Species: Pimepha/es promelns PpKCICR TesL Number: 39 Survival am/ Growth Data Day 1050 m KCI/L y z AA BB 0 10 10 10 10 I lo "'"' <o '\\c:A. s Sc.\\ (.,,\\.( 2 ~ I,:, s s *"- 3 ls ~ 'S s 4 \\o ~ ~*~ c.\\,A. 5 ~.A.. u\\. I" 3 ~ ~ '-\\ IA Id,, 6 ~ 3 7 -i'"- \\ 1:>J.. \\ '" \\ ul. A= Pnn weight (mg) Trny color code:: fo<~t ca<ee.n
14. q() 15.Ji /5.07 (5..3~
nalyst:
f:$
Dnte: Q4*.:2!:!- I~ 13
Pnn + Lnrvne weight (mg)
JG>. 11 1s.n 15. 73 15. CJC Analyst: TS Dute: 05.- IJ-l I{ C "" Larvnc weight (mg) A lfand enlculn1e(I. \\. 'l."1 o.S\\o o.,1o 0.,;., Anni, i: Xi Weight per initial number of lnrvn (mg)
C / lnitinl number of lnrvac A
i::t' 'O t-1 f-fnnd calculnted. {" 0 ~ Analyst: t,. ~>' o* 0. o* 1\\ vcrnge weight per i!1i1inl Percent 1111111be1* of lnrvnc (mg) rcduclion ().011 Slo,, ?. from control (%) Comment codes: c = clear, d = dead, fg. = fungus, k = killed, m = missing sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Co111111e11ts: Page 4 of6 Page 75 of94 OP AT21 6xhibil AT21.1, revision I 1 14
~ CQ CD
0) 0
"'II : tr1"11~t1Q.t°TP\\,1noSntrt.t1:llfl_1.lirl( ,.~ittfe:" '"""l l(Q) ii..~.... Wt,al~eJ 'ilN'A" A 10 Cot1trol B 10 C 10 0 10 [ 10 '300 f 10 Ii 10 H 10 I J.O -150 I 10 ~ 10 l LO M 10 600 N 10 0 10 p 10 Q 10 750 R 10 s 10 r 10 u 10 !IOO V 10 10 w X 10 y 10 1050 z 10 AA 10 OB to Ounnett's MSD nhte: O.OS1 PMSD* 9.0 llftal'""""'*~**' 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 9 7 7 8 8 * * ~
2 l
l l A tc,.111M1(n:tlfTICI 14 91 ISH 14 92 15,34 IS 45 l!i.89 1659 14.0l 1566 15~~4 IS22 lo.91 I0.60 IS.Bl 14-26 15.JI 1586 1580 15.40 LH2 15.25 103 IS.OS l!i.70 14 90 1S 22 1507 IS 3l MSD
PMSO ~
Pimepho/es pfome/os Chronic Reference To,cicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses ..,.". \\llrVlt LliMC.,.1fh1 fm:d :c Wflr,1 /t.ff.-...t--. ........,..,Jft\\l/.Jrll.t\\->*11( C..:ff1a..c,.tl:l!Wl11il1i.111 W*iJI)' / r,--tllll 1W*011t, oJ -1~1(11\\ll a ~ nw,r,i-~elf4,..,-..("lfl IIMMfOfl,;an,*t*jnlO 1'~"9........... 1,f'VM lffl&I 10.45 S.61 0561 0.5&2 10 75 SSl 0.5Sl 0.57i 49 O.S53 21 09 6.17 0617 0617 ll OS 5.11 0571 O.S71 ll.24 6.79 0.679 0 679 ll 19 & 30 0630 0.1164 4' 0.630 23.13 6.SA o.6SJ 06~4 2095 UJ 0.69! 0693 1139 5.73 O.S7! 0513 21.43 619 0619 0.598 H 0 619 20.87 5.65 0 56S O,S65 ll.2.4 6.33 0.633 0 633 20.70 6.10 0.610 0 610 ll.SO S.67 0 567 0.(08 49 0567 10..61 616 0 6'6 0.6JS 20.79 5.5& 0.610 11.SSS 20.01 4 15 0 593 0.415 1979 J.95 0 S64 0.57! 60 0.39~ 20.34 U4 0.618 0.49* 19.91 4.30 053! 0.430 17.71
uz 0630 0!52 17.20 l.47 061S 0.2A7 0.6lS 0 6l!l l7.St 1.54 OJS4 1824 2..s4 0635 0.15*
15.17 1 27 0635 0127 IS7! OS6 O.S50 0606 SI 0056 15.75 06& 0,660 0.06& 15.!IO 0.57 0.570 0057 M1nwnum SSnif,c.ant 01ft.,*nce Pe:rcen, Mtnlmum S1cnlflc1nt Offil!",:1nc.t Test number: 39 Tosi do101* l~*V08*15, 2018 fl,,1.-,.1ww,l*IO,J t,jlun....:til/h*lll_. CHff>dffllM-.,~i.;., ....,.,... t,.itadt011'11P""1 PILl,,,ltltrofl.. 'Wt. M (.ffltpi t~*J t,,,r) 100.0 0.576 4.9 Not 1pp.1*c.1ble 100.0 0.564 4.?
ISJ 100.0 o.s,a s.£

3.8
'7.5 0.593 6,2 -3,.0 75.0 0.431 u 74.7 40 0 0.2S2 1.3 56.3 12.S 0.077 44.4 80.1 PMSO ft* n,e,nure c,r ten prec.,.ski11 The PMSD 1s the ffilf'lm\\t.un perc*nt dlfftrr.nc.e batwt.'<<" 1he controt and uu tmont th.t c;1n ~ dfffiued stlti\\UC..tllV !'lfllfc.-lnt in.1 whole efflu.nl 101fKllV t~t '--"' PMSD bound dftl!fm,ood bV USfPA 00th r,e<<ennlel
12%
Upr,e, PM;D bound deu,......... d bv USEP~ 190th Plf<OftUlel
SO%
Lower ai11d up~, f'MSD boundJ. were determined from tht 10th and !Kl1h a,enentJlo, r-upec:uv,ly. of,MSO dilta hom EPA.i WET h'n.erlaboro1tofv ""'!abtl*rt Study t\\JSfPA 2.0011; USEPA, 'JOO lb) The lowor PMSO l>:Jl.ffld ~p,~or\\U a practle.ll 'llm1t lo tht.sens.ti\\n\\v of 1M tttl method *.,d 1J.11ot i IT"lfnlmurt" uupttrc* cnt*rlJ. llSEPAi ?OOh, 2C01b. ftt\\al Rep0rc lntf-rlabo,ato,; Viar,ab,,,ty S.t\\idyof EPA Sh0n*tt1ffl Chro"ic 1nd Aei.te Whote URu~r,t lor1e1ty ltst MHhod\\, V~omes l 1nd 2*App1nd1a ~PA*!i-2!*1*01--004 ~nd EPA*82l*B*Ol-00S. US Erw,ronmt.ntJI Proiac.hon ~ek\\! CM'lt1"11111I OH r..-. - .... ~* -r
Start Dal
518!2018 End Date 5115/2018 Sample Date Comments Co nc-m IL O*Control 300 450 600 750 900 1050 Cone-mg IL 0-Conlrol 300 450 600 "750

900
' 1050 Auxllla~ Tests 1 0000 1 0000 1 0000 1 0000 07000 0.4000 0 2000 Mean .0000 1 0000 1.0000 0 9750 0 7500 04000 0 1250 2 1.0000 1.0000 1 0000 1.0000 0 7000 04000 0 1000 N-Mean .0000 1 0000 0000 09750 07500 04000 0.1250 Statistical Analyses Larval Fish Growth and Survlvaf Test-7 Day Survival Test ID* PpKCICR Sampl ID, REF-RerTo,cant Lab ID ETS-Envir Teslong Sol Sample Type KCL-Po1as um chlol\\d Protocol* FWCHR*EPA-821,R-02-013 Te t Species PP-P,mephal s promel s 3 4 I 0000 1 0000 I 0000 1 0000 1 0000 1 0000 l 0000 0 9000 0.6000 0.8000 0.4000 0.4000 0 1000 0.1000 Transform: Arcsin sguare Root Rank 1-Talled Number Mean Min Max CV~/. N Sum Critical Rese 14120 1.4120 1 4120 0000 4 0 1 4120 1.4120 1,120 0.000 4 16 00 10 00 0 I 4120 14120 1 4120 0.000 4 18.00 10.00 0 1.3713 1.2490 1 4120 5.942 4 16.00 10.00 1 1 0492 0.9912 t 1071 6.363 4 10 00 10.00 10 06847 0.6847 0 6847 0000 ,4 t0.00 10.00 24 0.3572 0 3218 0.4636 19 861 4 10.00 10.00 35 Stallstlc Critfcal Skew Shap,ro-Wilk'!. Test 1nd1caies non-normal drstnbouon (p <= O 01) 0 87142 0 B96 -0 2B93 Egus,,tt or,:mance can!\\Ot be con,rmed H~othesls Testp -tail. o.osi NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670 82 Treatments vs 0-Conlrol Maximum Llkellhood-P robft Paramater Value SE 95% Fiducial Limits Control Chl*Sg Critical P-,alue Mu Sigma Slope 12 59B1 1 67874 9.30775,s 8884 0 o 015,3 g 48773 0.99929 2 9314 O 0793B lntercep
31 93 4 91886 -41 571
-22.289 TSCR 1 0 r Point Pro b lS mg/L 9S% Fiducia! U m l 09
eco, 2674 558 141 477 111 613 98 EC05 3.355 632.177 562.953 679.848 01 EC10 3 718 675.58 614. 186 71 8 592 0 7 EC15 3.984 706.535 650 845 746 S63 l:C20 4.158 732 145 661.06 770 106 ll: 0 C
EC25 4 326 754 855 707.608 791.441 8_ 05 EC40 4,747 815 253 775.803 851-554 i 04 ecso 5.000 853.891 816 6 893.659 EC60 5 253 894 36 856 198 941 279 03 EC75 5 674 985.921 920 361 1032 97 02 EC80 5842 995.882 945.635 1073.49 EC85 6 036 1031 9B 975 257 1123.55 O I EC90 6 262 1079.27 1013 04 1 190.82 00 EC95 66 5 1153 36 1070.6 1299.41 lO 100 1000 10000 EC99 7326 1306.36 11B5.27 1533.44 Oose mg/L Dose-Response Plot 0.9 08 _ 07 ~ 08 ~ ~ 05 2l o 4 .... OJ 02 0, 0 I ~ Page 77of94 Total Number 40 40 40 40 40 40 40 Kun 2 16 lter 3
Start Date. 518/201 B End Oat 5115/2018 Sample Dale: Comments. conc-m IL 0-Conlrol 300 450 600 750 900 1050 1 0.5620 06790 0.5730 0.6100 0
150 0 2520 0 1270 2
0.5530 0.6300 0 6190 0.5670 0 3950 02470 00560 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Test to PpKCICR Sampl 10. REF-R rToxlcani Lab ID ETS-Envir. Testing So S mple Type. KCL-Po1ass1um clilonde P otocol* FWCHR*EPA-821-R-02-013 Test Species* PP-P1mephales promelas 3 4 06170 0.5710 0 6540 0.6930 0 S650 0.6330 06360 0.5580 0 4940 0.4300 0.2 40 0.2540 00660 0 0570 Transrorm: Untransformed 1-Talled lsoto nlc Conc-m!i!/L Moan N-Mean Mean Min Max CV% N t.-Stal Crltlcal MSD 0-Control 0 5758 1 0000 0.5758 0.5530 0 6170 4.944 4 300 0 6640 1 1533 0.6640 0 6300 0.6930 4190 4 -3 919 2.290 0.0516 450 0 5975 1.0378 0.5975 0.5850 0.6330 5.617 4 -0.966 .290 0.0516 600 05928 1 0295 0 5928 0 5580 0 6360 6 190 4 -0 755 2 290 0.0516 750 04335 0 7529 0 4335 0.3950 0 4940 9 874 d 900 0.2.S 8 0.4373 0.251 0.2470 0 2540 1.312 1050 0.0765 0.1329 00765 0.0560 0.1270 44..400 4 Awdllary Tests St.alllsUc Crittc:al Shapiro-W,ll('s Test,ndicales normal d1stnbu1ion (p > 0 01 ) 0 89619 0.844 Elartlell's Test 1nd1cates !:9Ual vanances (e a O 96) 0 211117 1.3449 H)'.~lhesls Tes-qt-tall. 0,05! NOEC LOEC ChV TU MSOu MSDe MSB MSE Ou nelt's Test 600 >600 0.05157 0.08957 0 00602 0 00101 Treatments vs D-Control Llne~r Interpolation (200 Resamples) Point m!!IL so !COS 603 64 78 83 IC10 632.84 15.23 IC1 662 03 1 96 IC20 691.22 12.82 IC25 72042 14 65 IC40 800 82 9 95 IC50 851 98 5 42 0.8 01 06 s= J 05 2 C:, 04 i;' O 03 0.2 0 1 0 l! § 0 Page 78 of94 95% CLjExe) 281.16 639 87 592 01 66671 623.04 701 48 65800 736 94 686.2S 77090 769 45 927 68 83565 8 7 63 Skew -0 8198 26811 0 1775 10 0 4492 Q9 0 4973 08 -0.1471 07 -0.1673 06 .. 0.5 C: 0<1 &. 03 "'
02 a:
01 00 01
02
-0.3 Oose-Respon e Plot 0 500 woo Dose mg/L 1-ta I, 0.05 lev I or s gn,ficance Mean N-Mean 0.6199 I 0000 0 6199 1 0000 0.5975 0 96!9 0.5928 0 9562 0 4335 06993 0 2518 0 4061 0 0765 O 123d Skew Kurt 0 24992 -1 s;sa F-Prob di 0 01008 3 12 1500
-.. T
l Q Page 5 of6 Envtronmrnr~ltHtlng So'u1fot1t. Jnc.. l Species: Pimepha/es promelas PpKCICR Test Number: 39 Daily Cltemistry: Day (A.nolysl identilicd for cnch dai, performed pH D.O. and conductiv11v mcasurc111cn1s onlv.) 0 J 2 Analyst J-{~ H..! it.ff i>,.., HJ Concentration Parameter pl-I (S.U.) -:/.9~ ~.!2. ":'.f."e '+.u,q 9.01 -:;.-=15 DO (mg.IL) -=,. -=I 1 *°? "=/.e 1.4A ~.~ -=f.-'t Conductivity 31::/ ?.lo 3~Y (~1mhos/c111) I', CONTROL
Atk3linity
,. *~,:A (me. CaCOJ/L) S41 sq ~-'
lfardn
"~ (mgCaCO.i/L) g<o &~ "Temper:iture (°C) ""\\.-\\ ' \\:, '1.---\\. b "\\...tl* 1 -..-t., '"\\.'\\..t "\\..'"'* "\\ pH (S.U.) 8.03 '::/.[JI 7/-"'!'1 'l.\\l~ '().01-- 7--=!3 DO {,~*g/L) -:/.~ -:/. fo 7{.11 ~o '1-,r °1-'f 300 mg KCI/L Co11d11cli\\'i(y 8&2 85Co 04; I (11mhos/cm)
Tem 1>erature (*C)
.,_~., ""L'-1.\\., "'t.i.\\-1 "\\..o..l* 3, 1.-\\-'\\ L.-.\\-la pH (S.U.) 8.oz '1.32. ?."11 'll4 I 0.0'-l '=1---=F"i DO (mg/LJ !ts 1.e;- '1-<:f '\\..0 '+,')- -=,.~ 450 mg KCI/L Conductivity //Oo / I Jo toeo (u01hos/c111) "Tempcr:iturc (°C) '"\\..'-\\. le, "\\..'-4,,_ "'\\.<{, f '1..1.\\. s I.I.\\.'\\ -d-b pH (S IJ.) e.oz. ':{:~(3 -=1.. <f(,, ~.S1-" 'o.~ -=,.:::,5 DO (m£/L) -=i-B 7:.5 =1-"l u, p, 't.~ '"7-., 600 mg KCI/L Conductivicy 1380 1350 \\'?,50 (1tmhos/cm)
Temperature (0C)
'"\\>I. lo 1..1.{ -'-l '\\.I.{* 1 i,'{.S "1.'\\,, ""\\..'-\\- '1..- t>H (S.U.) 8.0"2. -:,. -:f. (J 71-'lS ~.'5\\4 ao5 ':f,bt::t DO (mg/L) "':lq +*5 e.o IP,8 ~-6 "?s 750 mg KCI/L Conduclivity {1tmhos/cm) /020 /b3o 1~20 "'Temperature (oC) r..-l* b 1..-t. 6 1.1{.1 "'I..<\\., s 'bt..f '-"'* (., pH (S.U.) '8-CPO -:/.80 7."'15 ~."'ll J1 8.Dla ':/. ":lo DO (mg/L) -:,.q 7#.5 f:3. 1 'ho ',t.G( "7,5 900 mg KCI/L Conductivity l'8"'ro lct.oo l~O (Junhos/cm) "Temperature (0C) ).'-\\. 6 1..'{. 3 1.~.* "\\.'\\* lo 1..'{, t 'l..c.t,S pl-I (S.U.) '8,01 ?.TB ":/:.?s 'l.c;,")- e.o'l -:,_? {;, DO (mg/L) ?. "i i-~ e., lP.e e.o 7-9 1050 mg KCIIL Conductivity "Zl~o 2180 AtOfD (umhos/c:m) "Temper:iture (0C) '1'-\\-b "l..~ *S 1.'-(.1 "1. 't. s i.L\\,t "l.~.s Initial Final Initial Final Initial fl'inal
Tcmpcrn1ures pcrfom1cd m ll1c time oftest i11itintion, renewal or tcnninmion by the analyst 1dentllicd in 1h Dail Renewal lnfom1:1tio111able loc ltd 011 Page I

alinity and hardness pcrfonncd by the mmlyst identified on che bench sheet specific for each analysis and transcribed 10 this bench shccl by: _
~ d.. Hftlll Page 79 of94 SOP AT2I - Ex.hibitAT21.1 revision 11 -01*14
l l l Page 6 of6 fnvitot1nutnt,I TaUt,q SoluUont.. l..c. pecic *: Pimep/wles promela PpKCICR Test Number: 39 Day (Analysl identified for each day, pcrfonncd pH, D.O. and conductivity measurements only,) 3 4 5 6 Analyst HS l'J;L., ~, N V,..; ,J..) i,l, rt.5' Concentration Parameter pH (S.U.) -::,..CJ.If +:so 1-,G\\S t,Vq Ai~ '+.~ eoo ":?.ff3 DO (nig/L) ?.';f +*"I
}.~
':\\-0 +.".l. '~.1- +.8 -., *'i Conductivity .305 ~"""' &01.., ?Jci) (µmhos/cm) J CONTROL
Alkalinity
'\\.... Ji. .. '\\_,.,,,t (mg CnCO:;/L) ~ l&>O
Hnrdncs
~~ &'-\\
~
(mg CaC0 3'L) "Temperature (OC) '"\\.~., "1..-\\. 'S "\\...L\\,, "'\\..'-l.b 1.1.\\. \\, "'1.'-',4 i.,"\\. f "'L'-\\.'-\\ pH (S.U.) 8.oLJ "'1."J,..:l ?.~ 'l.~~ 0.o'-1 ~!JO E?)D't-7/.135 DO (111g/L) -={,.3 +,'l ~-q ~.o '\\..u 'l..u, "l,1'1 -:[.4 300 m" K 1/L Con<luctivily Bt.18 151-9-t~ e2.; Cvmhos/c111)
Tempcrntur
'"\\_I..\\,g "'-'-'*3 "l_'\\. q '\\.,'-(. !) .,_~.\\a 1,..-.\\. s i.'-l '\\ "1.'-\\..b (oC) pH (S.U.) (i.03 +:1--\\., y_r,::; ".}.~ 8/)4 l)...ultJ e>.e>S -:is,, DO (nig/L} 1,8 ~.g ~.o u?.'l /}.".} ':\\. V, ~~ "-:f..fo 450 mg KCI/L Conducth*lly /110 11\\D ll\\O lo~ (~1mhos/cm)
Temperature 1.-'\\. ~
.,_'\\A 1A1 '1...'\\.t.o ""l!-\\-\\c, "-4.s 1...\\.'\\ 1..'-\\-~ ("C) pH (S.U.) 8 -03 "1-, tA 'i.o'5 /l.,Yl 0.0~ ~.lJ~ a.os- -=l-83 DO (mg/L) '":{. fj "l,.-:i- ~.o 'lo 41' ':\\:+ ,~~ -is Conduc1h<i1
600 mg KCI/L (umhos/cm)
/31JD 13qo \\660 1'3140 "Tempernture "1...Y-* "\\..~.~ "\\.,~-1 (OC) -i_'-(.S '-L~-b i....\\..lo °l"I,'\\ ,'-I.!, pH (S.U.) fj'.o3 ~.1-0 ~.o'5 1.se, a.o, ~-~'l A,'lu "":/-'Bo 00 (mg/L) f.9 "+,If ~.D (I."'.}- '-t8 ~ r-t,'1 t*S 750 mg KCI/L Conductivity /~10 ~'430 ll4'f0 I \\o:Z.O (µmhos/cm) 11-'f em pcrnture 1.~ *°' "l..4."' 1.tl.1 \\.'l* s "'\\...4-, '1...\\. ~ 1.. '-t* '\\ -i:-tS (OC) pH (S.U.) B.oz. 1-:~1 'il,o 'i- /)i,,'2, ,.°" ~J~2 8.03 1,. ts>l DO (mg/L) t-9 ~ .. 4:> "*o l:\\.o '},a '.\\.lo ~A -={.5 900 mg f CI/L Contluctivity /360 ltf3o ('lSO ll\\QJ (111nhos/c111) "Temperature 1..f& i..~\\. ~
l. ~. (I L~-1 t.'\\-1 1.4-1
"\\.-{.'i 1,t.\\.b (*C) pH (S.U.} 8.02. 1.~l ~.~ 1..~3 ~,() 1' 'l.(~(4 e.>.oY ~-Bs-DO (mg/L) tJ.o +.~ <?.o t".).1, l.t.e ~.'l-e>.O '::/.5 10-0 mg J Cl/ ConductiYI 2.ISO o()OO a1,o '-"o (umhos/cm)
Temperature 1.~.f 1.'-\\* )
1\\(,f L.~-~ 1..~-1 1,.L{.3 "t<i. D ,c.{.1 (oC) Initial Final Initial Pinal Initial Final Lnitial Final
~~'fl: eratures performed at 1hc lime of test initiation. renewal or tcm1ination by 1h analyst identified in the Daily Rcncwnl lnfon11otion table locatc~n Page I.

h ity and hardness pcrfonncd by the nnnlyst identified on the bench sheet specilic for each analysis and 1ronscribed to this bench shcc1 by:
~ Page 80 of 94 SOP A TI I - Exhibit A T2 l. I, revision 11-01-14
-u n, z ..J ao -Ill N u C"O "C I " Ceriodaphnia dubia Erwlfonmenc I Testfflg SoluOans, ln<.. Chronic Reference Toxicant Control Chart Source: In-house Culture 1.10.----.---,---.---.---r--..-----.,----,---,-- -r---r---,----,,----,---.---r--...--.,----,,----,---, Control Limits (+/- 2 Standard Deviations) 1.08 .. =------*--~--~------..:---------------------------------------- 1.06 --- 1.04 1.15 r*** **** ** * *** *~*r** *.. * , *.. *. * ************* * * ****** ***** ******t*tr*** Warning Limits 1.10 .. *~--.. --*-**- *'"'. **- **-**.....* 1.05 .. **-'* **-*-**-** **~**-**--*** 1.00 ..................,..............................., ************~*****~*******-******.. ********************** Test date 7-day IC25 = 25% inhibition concentration. An estimation of the sodium chloride concentration which would cause a 25% reduction in Ceriodophnia reproduction (calculated uslngToxCalc). Central Tendency (mean logarithmic I~ converted to anti-logarithmic values) Control Limits (mean logarithmic 1Ci5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic I~+/- 2 coefficent of variations converted to anti-logarithmic values) USE PA Warning Limits (mean logarithmic 1Ci5 +/- S11_10 converted to anti-logarithmic values, S"-10 = 10 1h percentile of CVs reported nationally by USE PA) Page 81 of94
In< co "' Test number Test date 7-day IC1s Toxcal Dl!termmauon 7-day 1(25 (g/L NaCl) 1 01-10-17 1.0723 0,0303 2 02-07-17 1.0641 0.0270 3 03-07-17 1.0641 0.0270 4 03-07-17 1.0614 0.0259 5 04-04-17 1.0654 0.0275 6 05-02-17 1.0691 0.0290 7 06-06-17 1.0614 0.0259 8 06-06-17 1.0518 0.0219 9 07-11-17 1.0631 0.0266 10 08-08-17 1.0810 0.0338 11 09-12-17 1.0527 0.0223 12 10-03-17 1.0540 0.0228 13 11-07-17 1.0528 0.02l3 14 12-05-17 1.0469 0 0199 15 01-09-18 1.0826 0.0345 16 02-06-18 1,0500 0.0212 17 03-06-18 1.0697 0.0293 18 04-03-18 1.0835 0.0348 19 04-03-18 1.0827 0.0345 20 05-08-18 1.0822 0.0343 Ceriodaphnia dubia hronic Reference Toxicant Control Chart Source: In-house Culture l og10 Conversion Anti-logarithm1C Values (g/L NaCl) Labor.110,y Calculated CV CT s CT Control Limits Warning limits CT - 2S CTt2S CT -2CV CT+ 2CV 0.0276 0.0031 l 0656 1.0506 1.0808 t.0515 1.0796 0.0278 0 0029 1.0660 1.0517 1.0804 1.05.26 1.0794 0.0276 0.0029 1.0657 1.0516 1.0800 1.0524 1.0789 0.0276 0 0029 1.0656 1.0515 1.0800 1.0524 1.0789 0.0274 0.0028 1.0652 1.0516 1.0791 1.0524 1.0781 0.0275 0 0028 1.0654 1.0516 1.0794 1.0525 1.0783 0.0276 0.0027 1.0657 1 0524 1.0791 t.0532 1.0781 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.0275 0 0029 1.0654 1.0512 10798 1.0520 1.0787 0.0277 0 0032 1.0660 1.0502 1.0820 1.0512 1.0807 0.0274 0.0034 1.0650 1.0435 1.0818 1.01195 1.0805 0.0272 0.0035 1.0646 1.0474 1.0821 1.0484 10807 0.0270 0.0037 l 0642 1.0463 1.0824 1.0473 1.0810 0.0269 0.0039 1.0638 1.0449 1.0832 1.0460 1.0817 0.027d 0.0042 1.0651 1.0446 1.0859 1.0458 1.0843 0.0270 0.0044 1.0642 10427 1.0862 1.0440 1.0844 0.0272 0.0045 10646 1.0430 1.0867 1.0443 1.0849 0.0273 0 0047 1.0649 1.0424 1.0880 1 0437 1.0861 0.0274 0.0048 1.0651 1.0420 1.0888 1.0434 1.0869 0.0275 0.0049 1.0655 1.0415 1 0899 1.0430 1.0879 10th Percentile CV Warning Limits CT. SA.10 CT+ SA.1U 0.9803 1.1508 0.9807 1.1513 0.9804 1.1509 0.9804 1.1509 0.9800 1.1505 0.9802 1.1506 0.9804 1.1509 0.9801 1.1506 0.9802 l.1506 0.9807 1.1513 0.9798 1.1502 0.9794 1.1497 0.9790 1.11193 0.9787 1.1489 0.9799 1.1503 0.9791 1.1494 0.9794 1.1498 0.9797 1.1501 0.9799 1.1503 0.9802 1.1507 Note* 7-day ICu = 25% lnhlb1clon concentrauon An ut,matlon of the sodium chloride c.oncentrc111on that would c:.use a 25% reduc1,on,n c,modophn10 reproduction (calculnted using ToMCalc) CT ~ Co?JHral lendency or the I Ci~ values S = Standarct deviation of the IC,s values. Control Limits = Mean logarithrolc IC>i :r 2 ~tandard dev,allon~ convened to antl-logarlthmtc values Warning Limits = Mean logaTlthmlc IC11 r 2CV ors. 16 corwened ro anli-logarothn"c values. S~ 10 ~ Stal'ldard cle,v,anon corresponding to the 10 1' pe,cenUle of CVS reported nationally by USEPA IS~ 111 : 0.08) cv = Coefficient of vanatton.
Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture C _ 35 0 QJ
,e iij
i E
]~ 30
------.-----*--**----*----*-------.-....,.~-,-----*
~ ~ 25 er: C *.::: 0 C. VI 20 USEPA Minimum Acceptance Criteria(> 15 offspring per surviving female) ... ~ C 0 o_ u 15 l C 0 IU IU > 0... C: QI *u
E 11/
0 u C: 0 u
J "C
0... ~ QI 0:: 0...... C: 0 u... 0 - 10 30 20 10 0 40 30 20 USEPA Maximum Coefficient of Variation Guidance Criteria(< 42%), NCDEQ Maximum (<40%) USEPA Lower PMSD Bound (>13%) USEPA Upper PMSD Bound Acceptance Criteria(< 47%) Control Reproduction, Coefficient of Variation (CV) or Percent Minimum Significant Difference (PMSD) PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and Is not a minimum acceptance criteria. Central Tendency (mean Control Reproduction, CV or PMSD) 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations)
""' f Control Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture ToxCal Oetermln;itlon Control Reproduction Control Reproduction CV Control Reproduction Test tolfspn11g/fem,1te) '"') Test number Tes t date Survival Mean CV MSD PMSO CT 95')(, Confidence Interval CT 95% Confid nee Interval CT 11') (of1Spring/fon1ale) I'*) 1 01-10-17 100 30.5 3.2 1.524 2 02-07-17 100 27.9 8.5 1.895 3 03-07-17 100 29.7 3.9 1.530 03-07-17 100 29.9 5.3 1.918 s 04-04-17 100 30.3 4.9 1.924 6 05-02-17 100 31.0 5.9 L894 7 06-06-l7 100 30.7 4.9 1.968 8 06-06-17 100 33.1 4.6 2.0SO 9 07-11-17 100 31.0 4.8 1.661 10 08-08-17 100 28.7 4.7 1.668 11 09-12-17 100 30.3 6.8 2.191 12 10-03-17 100 32.2 3.5 1.926 13 11-07-17 100 32.3 7.4 2.215 14 12-05-17 100 28.6 6.0 2.344 15 01-09-18 100 29.7 4.5 2.015 1 02-06-18 100 29.3 5.6
1.824 17 03-06-18 100 30.6 6.8 1.995 18 04,03-18 100 28.0 4.5 1.729 19 04-03-18 100 1.8 5.5 2.108 20 05-08-18 100 30.3 5.4 2.172 Note Control Survi,al = USE PA r111n1mun1" l acccplabtllrv cn1e,1a i'! 80% survival Control M an Rep,oducUon = USE PA n11nrmum t Sl ace ptab11t1v cr,te"a l lS orrmnng/survtv1ng remal CV = Coeffcien ol vanauon tor control renrod,,ction 1%)
CT-2S 5.0 30.4 27.7 6.8 30.2 27.4 5.2 30.2 27.4 6.4 30.2 27.4 6.3 30.2 27.4 6.1 30.2 27.4 6.4 30.2 27.4 6.2 30.2 27.3 5.4 30.2 27.3 S.8 30.1 27.2 7.2 30.2 27.3 6.0 30.2 27.3 6.9 30.2
27.
8.2
30.3 27.4 6,8 30.l 27.11 6.Z 301 27.4 6.5 30.1 27.4 6.2 30.l 27.3 6.6 30.2 27.4 7.2 30.3 27.5 USE PA ma,mum cv gwdance en e,,a (90° pe,centile) < 2 NCDEQ ma,mum CV acceptance cnttna < 0%.
MSO
M1n,mum s,gn,l1can1 dlffc, nee.
PMSD = ,cent ml"'n1um,gnlf,cant difference. CT+2S CT 25 CT+2S 33.0 4.8 2,3 7.4 5.8 33.0 S.l 2.2 8.0 5.9 3.0 5.1 2.2 8.0 5.8 331 5.1 2.2 8.0 59 29 5.1 2.2 7.9 58 32 9 5.1 2.3 8.0 58
32.
S.2 2.5 7.9 59 332 S.l 2.5 7.7 59 33.l 5.0 2.5 7.6 5.9 33.l 5.0 2.4 7.5 59 331 5.0 2.4 7.7 6.0 331 4.9 2.2 7.7 60 33.2 5 l 2.1 8.0 6 l 33 1 52 2.5 8.0 6.2 32 8 5.2 2.5 8.0 6 3 32 8 5.2 2.S 79 6.2 32 8 5.2 2.4 8,0 6.2 32 9 5.3 2.6 7.9 6.2 3:31 5.3 2.7 7.9 6.3 33.l 5.3 2.7 8.0 6.4 PMSO,s a measure or test prttr,on The PMSO" the mrn,111um pe,cen1 d,lrcrence beiween tie con1101 and 1tea1n,ent that canoed clared 1.,nlstlcal vs, nof,cant,n a wl>ole efflut.n to *Cl!Y test Lower PMSO bountJ dete1"1tned by U EPA ( 10' percenrile) > 13%. The low r PMSD bound rep~s n1,
llta tocal hmlt to t~e Sf!nsit1v,ty of rhe tesl metl>od and 15 not~ nll!lllt\\Um acceot nci? ttl~na.
pptr PM O bound acceprance ulteua d~1e1mtn d bv USfPA (90 perrenrne) <-47. CT
Central tenue11cy ol tilt reproduwon, CV or PMS0values 5.. Stanoard d ylatlon of the rep,oduct,on, CV or PM O., lues Test PMSD
('i.) 95% Confidence Interval CT-2S CT+2.S 4.3 7.3 4.4 7.4 4.3 7.4 43 74 43 7.3 4.3 7.3 .6 7.3 .6 7.3 4.6 7.3 4.6 7.3 4.5 7.4 4.6 7.4 11.7 7.5 4.5 7.9 .7 7.9 4.7 7.8 4.7 7.8 4.7 7.8 .7 7.8 <1.9 7.8
0 - C-:J-:: n odium Chloride Chronic Reference To icant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaplmia dttbia Page I o 6 CdNaCJCR #: 215 Di/11tio11 prepm*atio11 informatiou: Comme11ls: NaCl Stock INSS number: lNSS 11.,,, Stock preparation: I 00 g NaCI/L: Dissolve 50 I.? NaCl in 500 rnL Milli*O water. Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 IS 1 1 Diluent volume (ml.} 1491 1488 1485 1482 1479 Total volwne (mL) 1500 1500 1500 1500 1500 Test orgm1ism source i11fomwtio11: Test iuformntion: Organism age, I < 24-hours old Randomizinit template color Ke.l) Dote pnd times orgonisms were born I oS*bl *It' o~~o~ 0'\\Clb lncubnlor number and shelf between: lr,:-at1on: "2...l1. Culture boaro: ci:-u,-\\r.6.. Replicate number: I 1 I 1 I
I s I 6 I ' I s I 9 I 10 YWTbntch:
CullUrc board cup number. '-l , I f-I '\\ I '~, I~ I q I l f I *11. 1 ~ o'\\*H,-t e Transfer vessel in fom1ation: PH=,.'\\o s.u. Temperature= i.'\\,',*c Sele11n:r1ru111 batch: o~~ -l8 Average transfer volu111c (mL}. < 0.25 mL Daily reuewnl iuformatiou: Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or lrntch used t'!rn1ination time 0 05-08-18 Of-\\\\\\ OS-~*lf f:>.. A( I 05-09-18 (')Q~1 OS-~;~ ~ kt 2 05-10-18 o~'S.S OC.:.-m,.t~ ~ KC 3 05-11-18 \\J OhS1-OS~
l<! &
Y1 4 05-12-18 o'\\-i.i OS *U'\\
l r ti 5
05 18 oll\\1-~ OS-Cf\\*\\~ 1( 6 05 18 o~S1-O~* O"'\\* Lr/' \\1 7 05 18 OR \\"L- \\ ( Co11trol information: Acccptoncc criteria Summarv of tesl eudpoi11ts: % of Male Adults: Ol S 20% 7-day LCso "}, t./oo % Adults having 3'6 Broods: I I"\\(\\ 1 ~ 80% NOEC 1000 % Mortality: ()7. S20% LOEC Ii.OD Mean Offspring/Female: 3o., ~ I 5.0 ortspring/fcmolc ChV \\O(\\ $. $ %CV: S-~ 7. < 40.0 % ICzs 1042. 2. Page 85 of94 SOP AT l4 - Exhibit ATl4. l, revision I 1-01-14
l Page 2 of 6 pecies: Ceriodaplmia dubia CONTROL CdNaCICR #: 215 Survival a11d Reproduction Dain Replicate number Day 1 2 3 4 s 6 7 8 9 10 J Young produced 0 D 0 a C D 0 () 0 0 Adult mortality L_ \\..- L \\__.. L L 1 Voung produced ('\\ (J 0 n 0 n 0 0 0 [) Adull mortality \\...... L-L.. L.. L... L.. J Young produced 0 t'\\ C\\ 0 0 D 0 0 0 0 Adult mortnlity L L- \\.._. \\_.. \\__.. 4 Young produccu ~ ~ ~ '-\\ '-\\ ~ '-\\ "--\\ c; ~ Adult mortality L L \\...- L. \\....- L L \\'oung producctl I\\ \\":) \\t_ IC) \\0 I\\.) I\\ l \\ \\\\ Adult morlnllty L L. L L \\..._ G Young produced 0 6 l) 0 (') 0 {') (') 0 0 <lull mortality \\ 7 You ng produce(] 1<::. \\\\ \\~ \\\\.c, I\\, tg,s \\\\ \\ '-\\ \\ c; Total rom12 produced -z...'\\
>~
""1.. 'i 2)0 ~L.
>\\
"2°'. ~"1.... ~c 1-~ C. \\. Finni Adult Morlnlity L. '- X for Jrd Broods y 'x. '¥. "')('... ')(.. r-- 'i<:'.: )(_ x.. 'K. Note: Adult mono lit {L = live. D =dead), SB = split brood (sin°le brood split between two days). CO= carry over (offspring carried over with :tdult during transfer). Co11ce11lrnlio11: % Mortality: I Cl Mean Offspring/Female: I.::?)o.':l 600 mg aCI/L S11n1ival and Reproduction Dain Rcnlicnlc number Dar I 2 3 4 5 Ci 7 8 ') JO I Young produced 0 () () 0 /) a () 6 0 0 Adult mortality \\... \\..._ L. L L L.. L 2 You ng produced f"'I 0 n 0 0 0 0 () 0 0 Adult morr:ility \\... L L L L L L J Vo1111g prollnccd {') 0 0 0 0 () 0 C) 0 a Adult mort:ility l L \\.._. L L L L L 4 Young produced ~ s '--\\ ...., ~ "-\\ " s ~ ~ Adult mortnllty L L \\_ L L 5 Young produced \\\\ \\ "'L *~ 10 l~ IQ 10 11 Adult mortality \\.._ \\.._. L \\__ \\.._ G Voong produced 0 0 0 r,.. 0 6 0 0 0 0 dull mortolity \\... \\..._ L L \\. L \\.... 7 Young produced llo ,g 1$ ,s \\\\ \\q II,,,5 Totnl youn produced ~,
)'-\\
~, ~, ~~ ~ 31- .3"L ~t> "'l.~ Finni Adult 1\\lorlnlity L L L \\...... \\.... L \\..... L. Note: l\\dult mortality (L = ltve, D = dead), SB= spilt brood (smgle brood splil be I ween two days), CO= carry over (offspring cnrricd over with adult during transfer). Couceutration: % Mortality:
07.
Mean Offspring/Female: ~\\.b % Reduction from ContTOI: - '*\\.'~ 7_ Page 86 of94 SOP ATl4 - Exhibit AT14. I, revision 11 14
l a "" ~ Page 3 of6 Species: Ceriodaplmia dubia 800 mg NaCl/L Day 1 I Young produc d 0 Adult morlallty \\..- ? Young produced n Adult morlality \\..... 3 Young produced (") Atlult mortality \\._ 4 Young produced ~ Atlull morrnlity Young produced l\\ Adult mortality G Young produced 0 Adult mortnlily 7 Young produced l & 2 0 \\.._ 0 \\._ 0 I_. _""1., lo a Tolnl ouug produced
A
"':)-,_ \\_ Finni A<iuh !\\lorr:iliry CdNaCICR #: !!..= Survival and Reproduction Data Replicate number 3 4 5 6 7 8 9 10 a a D 0 D l'I (\\ (') L \\._ \\_ \\.._ \\,__ L.. \\_ 0 0 0 0 0 (") ri n \\.._ L. t) 0 D i\\ D 0 0 D \\._ '- '-{ ~ .s ~ ~ ~ ~ '-\\. \\_ L L \\0 \\Q I-,_ I\\ \\ '"l.. I~ I C) \\..... '- 0 0 0 ('I 0 0 0 0 L L L \\..._ L- \\ \\o l& t cs \\ '-\\ \\ I.:, 1 l.o \\ l.,
P
"'b:> ~L-. t...<\\ ~"'L ~ \\
)\\._
L \\_ '- - Note: dull mortality (L = live, D = dead} SB= splil brood (single brood split between Lwo days}. CO= carry over (offspring carried over with aduh during lrnnsfer) Co11ce11tmtio11: % Mortality: r'i'1. Mean Oftspring/Female: ~,.-, % Reduction from Conu*ol: -'-\\. b 1. 1000 ma aCl/L Survival anti Reproduction Data Rcoficnte number O~y 1 2 3 4 5 G 7 8 9 10 I Young J1rod111:cd C () 0 0 ('\\ (') () n 0 0 Adult mortnlity L L L l_ L L.. I._ L 2 Young produced 0 0 0 0 () {) 0 (') () 0 /\\dull morr:ili&y \\_ L-L L L \\_ L L L. 3 Young produced (') (') () 0 () (\\ ('i (', (\\ n Adult morrnlity \\...- \\._.. L- \\..- ~ Young produced '.\\ 5 ~ s ~\\ '-\\ ~ ~ ~ Adult morlalily L L L- \\._ L L L 5 Young produced \\ l ,o lQ I \\ IC) I "'"L \\ "L \\ "1.. ,o 10 Adult morrnlily L L L- \\..... G Young Jlroduccd 0 0 0 0 0 0 () 0 0 0 Adult morfnlity L L \\._ L L L.. L \\._ L 7 Young produced \\ ~
'-l I \\.I,,
I lo, -, tS l 1 \\ C\\,, Total young produced ~~ "'2-'\\ '"2., ""!)~ °3t) ':) "'.) '3 \\ ~1...
?) "t.
~o Final Adult Mort~lily \\_ L \\...... L Note: Adull monahty (L= hvc, D= dead}, SB =spht brood (smglc brood sphl between two days}. CO= carry over (offspnng carried over with adult during &ransrer) Co11ce11lratio11: % Mortality: Ol Mean Offspring/Female: 3 1.0 % Reducrion fiom Control: - 1. -l 7. Page 87 of94 S P ATl4 - Exhibit AT14. l revision 11-01-14
Page 4 of6 pecie : Ceriodaplmia dubin CdNaCICR#: 215 1200 CJ/L mg !'la S . I IR lll'Vll'tt Wll I eprot uctro11 DI (t {I Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young produced 01 D () t"i () 0 0 C) 0 r, Adull 111orl11llty L L \\....... ~ \\.- L \\._ 2 Young produced 0 a 0 0 c'\\ 0 0 0 0 0 Adult mortnlily L L-L \\...__ L Young produced n () 0 0 0 0 0 0 0 n Adull mortnlit)' L L L L.. L ~ Young produced ~ '--4. L. \\ ~ \\ Adull rnortnlily L L
5 Young produced t:
\\ ~ ~ \\o tn '\\ ,; '° Adult mortnllty G Young produced n 0 0 D 0 0 0 0 0 () /\\dull mortnlity \\.... '- \\....... L L,_ 7 Young produced g () b 0 D 0 -'\\ 0 ~ D Tolnl youn
produced
\\ °' \\0 \\ :> \\ ""L Is. \\ "'"'L ,s \\1- \\, \\ Finni Adult i\\lort:1litr L \\,._. '- \\._ '- Nolt!: Adult mort:ilil * (L = live. D
dead). SB = split brood (single brood split bcLwccn tw dny }, CO c carry over (olTspring corned over with adulL dnnng tmnsfcr).
Co11ce11trntio11:
1.
n.. s % Reduction from Control: ., 7. 1400 ma a 1/L Survival mu/ Reproductio11 Data Rcnlicnte number Dny J 2 J 4 5 6 7 8 9 10 I Young produced n r'J () 0 0 f) 0 /") ('"\\ 0 Adult mortality L-L- L L L L L. L 2 You11g pl'oduccd ('} () 0 0 0 0 () 0 0 0 Adulr mortality L L,_ L-L ~ L. L L J \\louug produced () /') n 0
11) 0
(\\ n (') () Adull mortality L L L L. L. L L. L L -4 Young produced \\. "L \\ \\ ~ \\ "'L. \\. \\ \\ Adnll morl:ilfry L L L. L L. L l L L Young protlnccd fl 0
2.
0 0 () c) L\\. ""L 0 Adult morlnlity \\ \\_ L \\.._ L-L L L L 6 Young produced (") 0 0 0 D () r, 0 n () Adult mortality L L L L L L 7 Yo_ung protluccd () (') 0 0 0 0 0 0 () Tolnl young produced \\ 1-l 3> \\ ~ \\ £... s ~ \\ final Adult Mortnlity \\_ L L. L.. L L. L Nole. Adult mor1nli1y (L
live D

dc3d), SB"" split brood (si11glc brood plit bctw en two dnys). CO.., carry over {offspring carried over with adllh during trnn fer}
Co11ce1tlratio11: % Mortality:
07.
Menn Offspring/Female: 2. ~ % Reducrion fi*om Control: '\\'2.. \\1. Page 88 of94 SOP Tl4 -ExhibitATl4. I, revision l l-01-14
~ CQ C1) 00 co 0,-.. f Envlronn1an1111TC1stlng Solutions, Inc. Control Dav 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 3 3 3 s 11 l3 12 6 0 0 0 7 15 17 13 Total 29 33 28 600 mg NaCI/L Day 1 2 3 l 0 0 0 2 0 0 0 3 0 0 0 4 3 5 4 s 12 11 t2 6 0 0 0 7 16 18 15 Total 31 34 31 800 mg NaCI/L Day 1 2 3 1 0 0 0 2 0 0 0 3 0 0 0 4 s 3 4 5 11 10 10 6 0 0 0 7 18 19 16 Tot.ii 34 32 30 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 4 3 4 10 12 10 10 0 0 0 0 16 16 18 15 30 32 31 29 Repllcilte number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 4 3 4 3 u 13 10 13 0 0 0 0 15 17 19 16 31 33 33 32 Replicate number 4 s 6 7 0 0 0 0 0 0 0 0 0 0 0 0 5 s 3 3 10 12 11 12 0 0 0 0 18 15 18 14 33 32 32 29 Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCI/L Total 8 9 10 Dav Replicate number Total 1 2 3 4 s 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 3 36 4 4 5 3 s 4 4 4 3 3 3 38 11 11 11 111 s 11 10 10 11 10 12 12 12 10 10 lOS 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 l7 14 15 156 7 18 14 14 17 16 17 15 17 19 17 164 32 30 29 303 Total 33 29 27 33 30 33 31 32 32 30 310 1200 mg NaCI/L Total 8 9 10 Oay Repllcate number Total l 2 3 4 s 6 7 8 9 10 0 0 0 0 l 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 5 3 3 37 4 3 3 2 4 2 2 1 .3 3 1 24 10 10 11 114 s 8 7 s 8 6 10 7 9 s 6 71 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 17 17 15 165 7 8 0 6 0 0 0 7 0 9 0 30 32 30 29 316 Total 19 10 13 12 8 12 1S 12 l7 7 125 1400 mg NaCI/L Total 8 9 10 Day Replicate number Total l 2 3 4 s 6 7 8 9 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 5 4 40 4 l 2 1 1 3 1 2 l 1 1 14 13 10 12 111 s 0 0 2 0 0 0 0 4 2 0 8 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 16 16 16 166 7 0 2 0 0 0 0 0 0 0 0 2 32 31 32 317 Total l 4 3 l 3 l 2 5 3 1 24
'l! CQ CD co C) 0.... f Envlronm@ntal T@sllng Solullo1,., Inc. Concentration (mg/L NaO) 1 l Control 29 33 600 31 34 800 34 32 1000 33 29 1200 19 10 1400 1 4 Dunnett's MSD value: 2.172 PMSD: 7.2 3 4 28 30 31 31 30 33 27 33 13 12 3 1 MSO: PMSD= Ceriodaphnio dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Repllcate number Survival s 6 7 8 9 10 (%) 32 31 29 32 30 29 100 33 33 32 32 30 29 100 32 32 29 32 31 32 100 30 B 31
32.
32 30 100 8 l2 15 12 17 7 100 3 l 2 5 3 1 100 Minimum Significant Difference Percent Minimum Significant Difference Test number: CdNaOCR 11215 Test dates: May 08*15, 2018 Average reproduction Coeffld entof Percent reduction (offspring/female) variation (%) from control (%) 30.3 S.4 Not applicable 31.6 4.8 -4.3 31.7 4.S -4.6 31.0 6.S -2.3 12.S 30.0 58.7 2.4 59.6 92.l PMSO ls a measure of 1esl precision. The PMSD,s the minimum percent difference between the control and treatment that can be declared s1atisticallv 5ignif,cant in a whole effluent toxicitv test Lower PMSO bound determined by USEPA (10 1 " percentile) = !3%. Upper PMSO bound determined by USEPA (90 1 " percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET lnterlabor'iltorv Variablh\\V Study (USEPA, 2001a; USEPA, 2001b). The lower PMSD bound represents a practical limit to the sensit1v1tv of the test method and,snot a minimum acceotance criteria. USEPA. 2001a, 2001b. fin3I ~eport: lnterlaboratory Variabllitv Study of EPA Sho11 -\\.erm Chronic and Acute Whole Elfhienl To~iclty Test Methods, Volumes 1 and 2-Appeoclix EPA-821-8-01*004 and EPA-821-8-01-005. US Environmental Protection A11ency, Cincinn.111, OH -... ;r
Statistical Analyses Cerlodephnia Survival and ReproducUon Test-Reproduclion Start Date* 5/8/2018 Test ID* CdNaCICR Sample ID REF-Rel Tolocent End Date* 5/15/2018 Lab 10* ETS,Envir Tesung Sol Sample Type NACL-Sod1um Chlor,de Sample Date* Protocol' FWCHR-EPA-821-R-02*013 Te& Species: CD-Cenoaapnn1a dub1a Comments Conc-mglL 2 3 4 5 6 7 8 9 10 0-Con of 29000 33000 26.000 30000 32 000 3 000 29.000 32 000 30000 29 000 600 31.000 34 000 31 000 31 000 33 000 33000 32000 32000 30000 29000 800 3" 000 32 000 30 000 33 000 32 000 32000 29000 32000 31 000 32.000 1000 33.000 29000 27000 33 000 30.000 33 000 31 000 32000 32.000 30 000 1200 19 000 10000 13000 12.000 8 000
12. 00 15 000 12 000 17 000 7 000 1400 1 000 4000 3000 1 000 3.000 1 000 2000 5 000 3000 1 000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-m9/L Mean N-Mean II/lean Min Max CV%
N Sum Critical D-Control 30 300 1.0000 30.300 26.000 33000 40 1 0 600 31 600 1 0429 31 600 29 000 34 000 4 764 10 127 00 75.00 800 31 700 1.0462 31 700 29.000 34 000 4 474 10 128 50 75 00 1000 31.000 1.0231 31 000 27.000 33 000 6452 10 118.SO 75.00
1200 12 500 0.4125 12.500 7 000 19 000 29 993 10 55 00 7500

1400 2400 00792 2 400 1 000 5000 59 577 10 55 00 75 00 Aux Illa!)'. Tests Statistic Critical Kolmogorov O Test 1n<.*cates normal dlstnbuuon (p > o 01) 061747 1 035 Banleirs Test indicates un!!9ual vanances !e = 8.62E-03) 15 446 15,0863 H~eothesis Test (1 -tall, 0.05)
NOEC LOEC ChV TU Steel's Many-Orie Rank Test 1000 1200 1095 45 Treatments vs o.control Linear Interpolation (2.00 Resample l Point IC05 IC10 iC15 IC20 IC25 IC40 IC50 mg/L SD 95'!. CL Skew 1014 7 7.02626 1001 38 1018 62 -3 9420 1031.57 4 98651 1015.85 1037 46 -0 7722 1048 43 5 27534 1036 35 1056 35 -0 3960 ,.o 1065 3 5.75815 1053 84 1075 32 -0 0539 0.9 1082.16 6.39113 1070.95 1094 63 0 19 3 11 216 8 83718 1118 91 t 152 13 04684 08 1166.4 10.7131 115017 1191 85 0 5230 07 ~ 0.6 0 05
a. 04 ti II'. OJ 02 0 1 0 0
-01 Dose-Response Plot 40 ~---------------------. 35 30 .2 25 tl j 'ti 20 E
0.
ti_ 15 10 5 0 +----""T""----,-----,.-----,-----1 ~ ~ Page 91 of94 0 500 1000 Dose m9/L Mean N-Mean 31 200 1 0000 31.200 1 0000 3 1 200 10000 31 000 0 9936 12 500 04006 2 400 00769 Skew Kurt 014429 1 55997 1500
Statistical Analyses E.nvJ,onm*"t*I fiiltln!a Soklllon,. f Used for PMSD calculation only. Ceriodaehnia Survival and Reeroduction Tes-t-Reeroduction Start Date 5/8/2018 Test ID. CdNaCICR Sample ID: REF-Ref Toxicanl End Date* 5/15/2018 Lab ID: ETS-Env,r. est,ng Sol Sample T pe NAC -Sodium chlond Sample Dat Protocol* FWCHR-EPA-821 -R-02-013 Test Species: CD-Certodaphnia dub,a Comments Conc-m9/L 1 2 3 4 5 6 7 8 9 to D-Conlrol 29 000 33.000 28.000 30 000 32 000 31 000 29.000 32 000 30.000 29.000 600 31.000 34.000 31.000 31 000 33.000 33.000 32.000 32.000 30 000 29.000 800 34 000 32.000 30 000 33.000 32.000 32.000 29.000 32.000 31 000 2.000 1000 33 000 29 000 27.000 33.000 30.000 33.000 31.000 32.000 32.000 30.000 1200 19.000 10 000 13 000 12.000 8.000 12 000 15 000 12.000 17.000 7.000 1400 1.000 4 000 3.000 1.000 3.000 1 000 2 000 5.000 3 000 1.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSO D Control 30.300 1 0000 30 300 28 000 33 000 5.401 10 600 31.600 1.0429 31 600 29 000 34.000 4 764 10 -1.369 2.287 2.172 800 31.700 1.0462 31.700 29.000 34.000 4 474 10 -1.474 2.287 2 172 1000 31.000 1.0231 31.000 27.000 33 000 6.452 10 -0.737 2.287 2.172 ' 1200 12.500 0.4125 12.500 7 000 19 000 29.993 10 18.744 2.287 2.172 '1400 2.400 0.0792 2.400 1 000 5 000 59 577 10 29379 2.287 2.172 Auxilla!l'. Tests Stati tic Critical Skew Kun Kolmogorov D Te t Indicates normal distribution (p ::. 0.01) 0.61747 1.035 0.14429 1.55997 Bartlett's Test tnd1cates unegu.al variances !e = 8 62E-032 15.446 15..0863 H:ieothasls Test j1-tall, 0.05) NOEC LOEC ChV TU MSOu MSDe MSB MSE F-Prob dt Dunnett's Test 1000 1200 1095.45 2 171 55 0 07167 1602.35 4 50926 5.2E-40 5, 54 Treatments vs D-Control Page 92 of94
l l 0 C Pa.,e 5 of6 Species: Ceriodaplmia dubia CdNaCICR #: 215 D *1 Cl. auy zenus 1y: D a (Analyst identified for each da,, pcrfonned pH. 0.0. and conductivity mcasurcmcnts only.) 0 I 2 A nalys t rl.r H5 H.5 tlv k..J rlS' Con ce ntration Parameter pH (S.U.) +.e,4 7/.9'.5
/.9(3 Q;.o,
e.o l 8.oo DO (mg/L) --:,.. =1 1-*B '1 * (:J .,.~ ,i. "1-f*B Contluclivlty 3 /t-3 10 52"' (iunhos/cm) CONTROL
Allrnlinity S'\\
SC\\ ~'-.t (mg CnCOi/L) " I li1rdncss ~lo g(o v* "" (me. CaC01/L)
Tempcrnturc (0C)
'"l.-\\. i- ""\\J,
0
"'\\.'°(*"\\ ~.\\ "'l.'\\.& ""\\.S. ' pH (S.U.) R.,v, ":l..,,~ ":/-. Cf I '+.~5 ~ l )lA -:/.9b DO (mg/L) 7,.:, -i,5 ":(. 'I] ~.+ ~.ll 7-e 600 mg NaCI/L Co11duc1h*ity IL/:JO 1390 l'f0° (µmhos/cm) "T cmpcr11turc (oC) ""\\.."-\\-'\\ 1.L\\. f 1..-l. &- ""\\>L~ "'l.i.\\..'\\ "'\\.~. 0 pH (S.U.) R -oo ':l-9Z ?.93 'l-.t:\\v 0.04 °'"::/-'7~ 00 (mg/L) +8 --=,.e, -:,.. er '+.e t:\\I 71-e 800 mo NaCI/L Conductivity /(j()() l f3 /0 ,~o (µmhos/cm)
Tcmpcrntui-c (°C)
""\\...\\*.So "1.'{
8 iJ.l..f'
-u-\\.i 1.'t, '\\ -is.,_ pH (S.U.) 7/.qq "1. 413 -:,. 9 3 '\\~S' S.04 ?-9~ DO (mg/L) ~ '1 -=f. g ?..<t '+~ '+,Ii" ":/-EE I 000 mg NaCI/L Contluclivfl * = (µ mhos/cm) Z l'Bo "2 ltoO a.Hoo
T emperature (oC)
"'l..S.o ""\\.S. o .... t.\\." lS.I "'l...'{,'l ,s..,_ pH (S.U.) "7/. 'J"t 1-'12 ":{. 93 'l-!l4 0~ ":f..9J.f DO (mg/L)
8. o 8-o
-=,."' '},e ~~ -=,.. 7 1200 mg NaCl/ Contluctivity 2"-1&/o zsoo asaJ (urn hos/cm)
T emp1:rnturc (0C)
'\\.'-l.c:.; "bl,."\\ "'\\.4,, "L*I. '\\ l'\\."\\ b.O pH (S.U.) "'1-. "16 -=,. '12.. -:,. "!:1 l'}.~..cr e.o3 -=,.. 9'1 DO (mg/L) f?, L ~o ff..;, ~-q ~~ -=,.=, 1400 mg NaCI/ L Conductivity 2 9 ()() 2 B "7o ~eqo (µmllos/cm)
Temperature (0C)
"1.'*l.'l "1..-l,'\\
i. '-l. &-
""\\.S. 0 '1.-t.1 '15 -D Initial Final Initial Fina l lnitial F ina l
icmpcraturcs performed al the time of test initiation, renewal or 1cm1mation by the analyst identified in the Daily Renewal lnfonnation table located on Page I. Alkalinity and hardness perfom1cd by the analyst identified 0 11 the ben h sheet specific for each analysis and transcribed to this bench sheet by:
6, Page 93 of94 SOP AT14 - Exhibit ATl4.1, re ision 11 14
I I l I I I I I J J Page 6 of6 pecies: Ceriodapl111ia dubia CdNaCICR #: 215 Day (Analyst identified for each day, performed pH. D.O. nnd conductivity measurements onlv.l 3 4 5 Analyst Hf 14::.1.., P..c.c... J.... - IA./ J._) Concentration Parameter 11H (S.U.) +.91 ,.,,gs "J..~s-e,,o4 0.\\18 e.ol e,.oo DO (mg/L) t *'1 '!,o f-,lc, 1..6 ".J..'} ~-q 'la Conductivity 305 ..? "'i &>0 !>oo (umhos/cm)
Alk:ilinily
~ ..,a CONTROL "'a ~ " (m~C:iCO.i/L)
Jfardnl' O'""'
r ~ &'-\\ (mit CaCOJ/L) .. Temper:iture "t..'-\\.g '"'!. s.\\ 1.-\\* ~ "V>>. 0 '1.A.1 "'\\S,. 0 ,._4., (°C) pH (S.U.) P..oo -i...&5 t-.q\\ 6.0\\ ~.Ol ~.q"} a.02. DO (mg,'!..) 7 -'":/- '3-0 1-,~ 'l.e '+~ 'l, ~ ~~ 600 mg NaCI/L Contluctivity l~~o 1300 l~ b (11mhos/c111) t:Ffo
T empernture
'\\. '-\\.. '\\ "l..-\\. ~ "l."\\. g '1..'-\\ *" , A.i "'lS", 1.. i..S.O (oC) pH (S.U.) ~.ol 1-.i i 1-.q 1 A.b O 8.0 1 'i-~:\\- e.o3 DO (mg/L) 7/.B ~.o 1-.<t" '1,8 "1--8 ~ -q t:\\A 800 mg NnCI/L Contluclivily / BE,o 1~<>0 llvo 1euo (11mhos/cml
Temperature LJ-\\.'\\
'-~* ~ t.**V\\ 1.-\\.~ 1.~-1 u.o tS -0 ("C) pH (S.U.) 8.a1 +,iq +.'l'l q.qq P,.L)3 ').~"} (3,0,{ DO (mg/L) 7.6 ~~ +-'l '1-4 ' ~fl ~A f>,O 1000 mg NaCI/L Conducth*ity 2210
1.1 <, 0
~130 "~c (u111hoslc111) "Temper:iture '"l..1.\\.-cf 1.A.'\\ c,\\.g '"l..<;. \\ L'1*1 "\\S.D i,S.Q (*C) pH (S.U.) '8.00 1-.q\\ t.~ ~kl. e,.o~ '+.'\\"}- ~.o4 00 (mg/L) e.o +J:i 1-A /.}(J ~ e,,o 8'° 12 0 mg Na 1/L Conduclivity -z..!,--eo ~510 ~SOo ~$lo <umhos/cml
Tcmpernture
'\\..'-\\.. g- '1..S. \\ ""t'-\\. i c*C) '\\.'-\\. f '\\_~., \\$. \\ -i.s..o pH (S.U.) 80 1 '5',00 +.'i!1- ').(\\<,, (!) l'f2. I). 41A d.04 DO (mg/L) &-o "'1-Z 'if.o rJ.0 ~A e.o e>JJ 1400 mg NaCI/L Conductivity 2 9 ?"0 ~85o '-wo ~q"'n (um hos/cm)
Temper:iture 1-L\\.~
"l.~.'\\ '1.'-\\. g , A.(
1. ~-'\\
~. \\ 1.~.o ("C) Initial Final Initial Final Initial Final Initial .,.e111pcrmures performed ot thc time oftest ini1ia1ion, renewal or termination by the analyst identified in the Dail Renewal lnformntion !oble located on Page I. Alkalinity and ha dncss perfonned by the analyst identified on the bench sheet specific for each anal sis and trnnscribed 10 this bench sheet by: _ SOP ATl4 - Exhibit ATl4. I, revision 11 14 6 /4.S 1.f!~ -:Z.q -i.s. "L -:J.~ -:i. Cf "L*l.'\\ 1.e::, "':f.<1.f t.S.o '"=1-8+- -:/. C, ""\\.l.\\,'i -=,. rn-fg. I '1.S- \\ ':/-8'1 R. t 1..~.f Final}}

ML18198A129

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