ML18198A129
| ML18198A129 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/13/2018 |
| From: | Anthony Williams Tennessee Valley Authority |
| To: | Office of Nuclear Reactor Regulation, State of TN, Div of Water Pollution Control |
| References | |
| NPDES Permit No. TN0026450 | |
| Download: ML18198A129 (102) | |
Text
{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 July 13, 2018 Chattanooga Environmental Field Office Division of Water Pollution Control 1301 Riverfront Parkway, #206 Chattanooga, Tennessee 37402-2013 TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR June 2018 Enclosed is the June 2018 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the reporting period . The final report for toxicity sampling conducted at outfall 101 , from May 13 - May 18, 2018, is enclosed . No adverse effects were observed . The corrected May discharge monitoring report for outfall 101 T is also enclosed . If you have any questions or need additional information , please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fi *
- ment for knowing violations.
t ony ~- illiams Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures) : U.S. Nuclear Regulatory Commission Attn : Document Control Desk Washington , DC 20555
PERMITIEE NAME/ADDRESS {Include Facility NameA.ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR Form Approved . Name _ _!YA - SEQUOYA!:!._NUCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01) Address P.O. BOX2000 - - - - - - - - - - - - - _ _ ..J!NTEROFFICE OPS-5N-SQN)_ _ _ _ _ _ _ _ TN0026450 101 G F - FINAL t ~rG _ _ _ SODDY - DAISYJ.N 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Ip~~t Fard!J!y TVA - SEQUOYAH NUCLEAR PLANT _ _ _ _ _
~
EFFLUENT Location HAMILTON COUNTY _ _ _ _ _ _ _ _ _ _ 0 ATTN :Millicent Garland From IYEAR 18 I MO 06 D 01 To 18 MO 06 I PAY 30
*** NO DISCHARGE D ***
NOTE: Read instructions before complelinA this form . PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ******** ******** ******** ******** 39.9 0 30 / 30 RCORDR MEASUREMENT ** 04 CENTIGRADE 00010 1 0 PERMIT
******** ******** **** ******** ******** Req. Mon. DEG.C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS TEMPERATURE, WATER DEG.
CENTIGRADE SAMPLE MEASUREMENT
******** ******** 28.0 04 0 30 / 30 MODELO 00010 z 0 PERMIT ******** ******** ******** ******** 30.5 DEG. C. CONTI CALCTD REQUIREMENT INSTREAM MONITORING DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ******** 2.6 0 30 / 30 CALCTD MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 EFFLUENT GROSS s PERMIT REQUIREMENT
******** ******** -** ******** ******** 3.0 DAILYMX DEG. C. CONTI NUOUS CALCTD FLOW, IN CONDUIT OR THRU SAMPLE ******** 1863 ******** ******** ******** 0 30 / 30 RCORDR MEASUREMENT 03 **
TREATMENT PLANT 50050 1 0 PERMIT ******** Req. Mon. MGD ******** ******** ******** **** CONTI RCORDR REQUIREMENT EFFLUENT GROSS DAILY MAX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE ******** ******** ******** ******** 0 30 / 30 CALCTD MEASUREMENT 03 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. ******** MGD ******** ******** ******** MGD CONTI CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO AVG NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** ******** 0.016 0.035 0 23 / 30 GRAB MEASUREMENT ** 19 50060 1 0 EFFLUENT GROSS VALUE PERMIT REQUIREMENT
******** ******** **- ******** 0.1 MOAVG 0.1 DAILY MAX MG/L FIVE PER CALCTD WEEK TEMPERATURE - C, RATE OF SAMPLE ******** 0.2 ******** ******** 0 30 / 30 CALCTD MEASUREMENT 62 **
CHANGE 82234 1 EFFLUENT GROSS 0 PERMIT REQUIREMENT
******** 2.0 DAILYMX DEG C/HR
/
~ --- l)
/
**** CONTI NUOUS CALCTD NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared uf~my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified ersonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the pe on or persons who manage the system, or those persons directly responsible for gathering the ~ident information, the information submitted is , to the best of my knowledge and belief, true, accurate, 423 843-7001 18 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injections occurred: Flogard MS 6236 (max calc. was 0.033 mg/L, limit -- 0.20 mg/L), Spectrus BO 1500 (max calc. was 0.037 mg/L, limit -- 2.0 mg/L) . EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name _ 3YA - SEQUOYA~NUCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01) Address P.O. B0X2000 - - - - - - - - - - - - - _ _ _(!NTEROFFICE OPS-5N-SQNl _ _ _ _ _ _ _ _ TN0026450 101 T F - FINAL _ _ _ SODDY-DAISY...!.N 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 FacJ!i!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ EFFLUENT Location HAMILTON COUNTY _ _ _ _ _ _ _ _ _ _
¥0o~iS81 NG _ p;-~-~-E-M,,,_o D ATTN :Millicent Garland MO 06 r 01 1 To [ 18 r 06 ~ - ~ - - ~ -30
_ D"""" Ax_
-~
*** NO DISCHARGE ***
NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring ******** ******** MEASUREMENT ** 23 CERIODAPHNIA Not Required TRP3B 1 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** Monitoring ******** ******** MEASUREMENT ** 23 PIMEPHALES Not Required TRP6C 1 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MIMINUM ANNUAL SAM PLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT
....,.,,.,..,..,_..,.,, __.,,.,,_,~-. -~
PERMIT REQUIREMENT
,,/""
~ )
/ /
~d~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified P. sonnel Anthony L. Williams properly gather and evaluate the information submitted. Based on my inquiry of the p son or persons who manage the system, or those persons directly responsible for gatherin the information, the information submitted is , to the best of my knowledge and belief, tru ccurate,
,,..- _§Je ice res1 ent 423 843-7001 18 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false informauv, , ;:,IGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was not sampled in June 2018. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
i PERMITTEE NAME/ADDRESS Name _ (Include Facility Name/Location if Different) _2VA - SEQUOYA!:!.._NUCLEAR PLANT _ _ _ _ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) MAJOR Form Approved . 0MB No. 2040-0004 (SUBR 01) Address P.O. BOX2000 - - - - - - - - - - - -
- _ _ _J!NTEROFFICE OPS-5N-SQ!:!.l _ _ _ _ _ _ _ _ TN0026450 103 G F- FINAL
_ _ _ SODDY-DAISY....I.N 37384 - - - - - - - - LOW VOL. WASTE TREATMENT POND FacJ.!!!y TVA - SEQUOYAH NUCLEAR PLANT - - - - - EFFLUENT Location HAMILTONCOUNTY - - - - - - - - - -
*** NO DISCHARGE D ...
ATIN:Millicent Garland NOTE: Read instructions before completinA this form . PH PARAMETER iX SAMPLE AVERAGE QUANTITY OR LOADING MAXIMUM UNITS MINIMUM 6.5 QUALITY OR CONCENTRATION AVERAGE MAXIMUM 6.7 UNITS 12 NO. EX 0 FREQUENCY OF ANALYSIS 5 / 30 SAMPLE TYPE GRAB MEASUREMENT 00400 1 0 PERMIT
******** ******** ** 6.0 ******** 9.0 SU ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ******** ******** ******** <2.5 <2.5 0 1 / 30 GRAB MEASUREMENT ** 19 00530 1 0 PERMIT
******** ******** ** ******** 30.0 100.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX MONTH OIL AND GREASE SAMPLE ******** ******** ******** <5.0 <5.0 0 1 / 30 GRAB MEASUREMENT ** 19 00556 1 0 PERMIT
******** ******** ** ******** 15.0 20.0 MG/L ONCE/ GRAB REQUIREMENT EFFLUENT GROSS MOAVG DAILYMX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.022 1.114 ******** ******** ******** 0 4 / 30 INSTAN MEASUREMENT 03 **
TREATMENT PLANT 50050 1 0 PERMIT REQUIREMENT Req. Mon. Req. Mon MGD ******** ******** ********
. ONCE/ INSTAN EFFLUENT GROSS MOAVG DAILYMX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT __.,..-- .--
*-~~-*.....
"\
NAMErrlTLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams
'"~
'direction""'" ""'"""' ,_ .. '"" ,~ ""' "' ~
or supervision in accordance with a system designed to assure that qualifie ersonnel properly gather and evaluate the information submitted. Based on my inquiry of the erson or persons who manage the system, or those persons directly responsible for gather" g the
/
~ i c e ~sident L
/
TELEPHONE DATE information, the information submitted is , to the best of my knowledge and belief true, accurate, 423 843-7001 18 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false i o~ion--- SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) Form Approved . MAJOR Name _ ..!_VA - SEQUOYA~NUCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01 ) Address P.O. B0X2000 - - - - - - - - - - - -
- _ _ _.{!NTEROFFICE OPS-5 N-S~l- _ _ _ _ _ _ _ TN0026450 110 G F - FINAL
_ _ _ SODDY - DAISYJN 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER FaclfilY TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMIL TON COUNTY _ _ _ _ _ _ _ _ _ _ EFFLUENT MO *** NO DISCHARGE Ixx I *** ATIN :Millicent Garland 06 NOTE: Read instructions before completin;i this form . PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT
******** ******** .. ******** ******** 04 CENTIGRADE 00010 1 0 PERMIT REQUIREMENT
.. ******** ******** REPORT DEGC CONTIN CALCTD EFFLUENT GROSS VALUE DAILY MX uous TEMPERATURE, WATER DEG.
CENTIGRADE SAMPLE MEASUREMENT
******** ******** .. ******** ******** 04 00010 z 0 PERMIT ******** ******** ** ******** ******** 30.5 DEGC CONTIN CALCTD REQUIREMENT INSTREAM MONITORING DAILY MX uous TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** ******** ******** ********
MEASUREMENT ** 04 UPSTRM DEG.C 00016 1 0 PERMIT REQUIREMENT
.. ******** ******** 5 DEGC CONTIN CALCTD EFFLUENT GROSS VALUE uous FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT
******** 03 ******** ********
DAILYMX 50050 1 0 PERMIT REQUIREMENT
******** Req. Mon. MGD ******** ******** ********
.. CONTIN RCORDR EFFLUENT GROSS VALUE DAILYMX uous CHLORINE, TOTAL RESIDUAL SAMPLE ******** ******** .. ******** 19 MEASUREMENT 50060 1 0 PERMIT
******** ******** ******** 0.1 0.1 MG/L Five per CALCTD REQUIREMENT EFFLUENT GROSS VALUE MO AVG DAILYMX Week TEMPERATURE - C, RATE OF SAMPLE MEASUREMENT 04 CHANGE 82234 1 0 PERMIT REQUIREMENT
******** 2 DEGC ******** ******** ******** . CONTIN CALCTD EFFLUENT GROSS VALUE DAILYMX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT
/ ---- )
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams
'" """' ""'""' ,~ '""'"a=-... "'"""'m'""- ""':f direction or supervision in accordance with a system designed to assure that qualified rsonnel properly gather and evaluate the information submitted. Based on my inquiry of the rson or persons who manage the system, or those persons directly responsible for gather g the
/
information, the information submitted is , to the best of my knowledge and belief, rue, accur ~
~esident-
/
423 TELEPHONE 843-7001 18 DATE 07 11 S ite Vice President and complete. I am aware that there are significant penalties for submitting false in n, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved . MAJOR Name _ _ TVA-SEQUOYA~NUCLEAR PLANT---- DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01) Address P.O. BOX2000 - - - - - - - - - - - -
- _ _ _QNTEROFFICE OPS-5N-SQ!fl_ _ _ _ _ _ _ _ TN0026450 110 T F - FINAL
___ SODDY - DAISYJ.N 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Faq!!!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMILTON COUNTY - - - - - - - - - - EFFLUENT YEAR MO o MO PAX Ixx I *** ATTN:Millicent Garland From ~ 18 I 06 : 01 To [ 18 - 06 I 30 *** NO DISCHARGE NOTE: Read instructions before completinQ this form . PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA SAMPLE MEASUREMENT
******** ******** .. ******** ******** 23 TRP3B 1 0 0 PERMIT ******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR PIMEPHALES SAMPLE MEASUREMENT
******** ******** .. ******** ******** 23 TRP6C 1 0 EFFLUENT GROSS VALUE 0 PERMIT REQUIREMENT
******** ******** **- 42.8 MINIMUM
******** ******** PERCENT SEMI ANNUAL COMPOS SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT v-PERMIT REQUIREMENT
/ )
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams
'C"~ ""'" '"""'"'~*"-' "°'"~""'-, -**~-1~*
properly gather and evaluate the information submitted. Based on my inquiry of e person or persons who manage the system, or those persons directly responsible for gat ring the information, the information submitted is , to the best of my knowledge and beli true,~
/
m, direction or supervision in accordance with a system designed to assure that quali
- d personnel
~_,
~
/
Site Vice President 423 TELEPHONE 843-7001 18 DATE 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name _ _!YA - SEQUOYA!:!__NUCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01) Address P.O. BOX2000 - - - - - - - - - - - - - _ _ ...1!.NTEROFFICE OPS-5N-SQ!il _ _ _ _ _ _ _ _ TN0026450 118 G F - FINAL ___ SODDY - DAISY....!.N 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER FacJ!!!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ EFFLUENT Location HAMIL TON COUNTY _ _ _ _ _ _ _ _ _ _ MO ¥0~J981NG iP,...,.,£~.......~.....¥_...MO......._D"""'"AY._ Ixx I *** ATIN :Millicent Garland 06 I 01 ] To l~1a~ I_o_s~_3_0~
*** NO DISCHARGE NOTE: Read instructions before completinQ this form.
IX PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT
******** ******** .. ******** ******** 19 00300 1 0 PERMIT
******** ******** **** 2 ******** ******** MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT
******** ******** .. ******** ******** 19 00530 1 0 PERMIT
******** ******** **** ******** ******** 100 MG/L TWICE/ GRAB REQUIREMENT EFFLUENT GROSS DAILYMX WEEK SOLIDS, SETILEABLE SAMPLE MEASUREMENT
******** ******** .. ******** ******** 25 00545 1 0 PERMIT REQUIREMENT
.... ******** ******** 1 MUL ONCE/ GRAB EFFLUENT GROSS MONTH FLOW, IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT 03 ******** ********
DAILY MX 50050 1 0 PERMIT MGD
- ONCE/ ESTIMA REQUIREMENT Req. Mon. Req. Mon. ******** ******** ********
EFFLUENT GROSS MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT /~ /~ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams
""""""' -*"' ,_. ~.*~*'""--* -*
, ~
/
direction or supervision in accordance with a system designed to assure that qu ,tied personnel properly gather and evaluate the information submitted. Based on my inquiry the person or persons who manage the system, or those persons directly responsible for thering the information, the information submitted is , to the best of my knowledge and lief, true,
..,,, ~ n t
/
423 TELEPHONE 843-7001 18 DATE 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting fa
- ation, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period , there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITIEE NAME/ADDRESS (Include Facility Name/1..ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved . Name _.!._VA
- SEQUOYA~NUCLEAR PLANT _ _ _ _ DISCHARGE MONITORING REPORT (DMR) 0MB No. 2040-0004 (SUBR 01)
Address P.O. B0X2000 - - - - - - - - - - - -
- _ _ _.(!NTEROFFICE OPS-5N-SQtil_ _ _ _ _ _ _ _ TN0026450 101 T F - FINAL
_ _ _ SODDY - DAISY,_I.N 37384 - - - - - - - - PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fac:J!!!y TVA - SEQUOYAH NUCLEAR PLANT ____ _ Location HAMILTON COUNTY _ _ _ _ _ _ _ _ _ _ EFFLUENT MO 05 NO DISCHARGE D ***
!x ATIN:Millicent Garland NOTE: Read instructions before completinQ this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** ******** 3 / 180 COMPOS MEASUREMENT ** 23 0 CERIODAPHNIA TRP3B 1 0 PERMIT
******** ******** **** 42.8 ******** ******** PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ******** ******** >100.0 ******** 3 / 180 COMPOS
** ******** 23 0 MEASUREMENT PIMEPHALES TRP6C 1 0 PERMIT REQUIREMENT
.... 42.8 ******** ******** PERCENT SEMI COMPOS EFFLUENT GROSS MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT
~
/
./
-------- /
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Anthony L. Williams Site Vice President
, '"~ """'"""" * ,~ .. ,,;,'°'"mom ,oo" '"'~-* - ~
direction or supervision in accordance with a system designed to assure U,at qualifie properly gather and evaluate the information submitted. Based on my inquiry of u, arson or persons who manage the system, or those persons directly responsible for gal ing the information, the information submitted is , to the best of my knowledge and rsonnel
,et. true, accurate and complete. I am aware that there are significant penalties for submitting alse informatio
~
~ --
Site Vice President SIGNATURE OF PRINCIPAL EXECUTIVE 423 TELEPHONE I 843-7001 18 DATE 07 11 TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA CODE I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled May 14 - 18, 2018. The final report will be included with the June DMR. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
TENNESSEE VALLEY AUTHORITY TOXI JTY TEST REPORT Report Date: June Ol, 2018
- l. Faci lity I Discharger: equoyah Nuclear Plant / TV
- 2. County/ tate: Hamilton / Tenn s e
- 3. NPDES Pennit #: TN0026-l50
- 4. Typ of Facility: Nu !ear-Fueled Electri Generalin!! Plant
- 5. Design Fl w (MOD): 1.579
- 7. 1Ql0: .).49l
- 8. OutfallTested: 101
- 9. Dates ampled: Mav 13 - 18.2018
- 10. Average Flow on Day ampled MOD: 1805. 1810. 1788 l l . Pertinent ite Conditions: Pr duction / o peration data will be pro i<l"'d up
- 12. Test Oat s: Mav 15 - 22. 2018
- 14. Test Species: Fathead Minnows (Pimephales µtome/as)
Daphnids ( eriodaphnia d11bia)
- 15. Concentration Tested %) :
Pimephale promelas: UV treated utfall l Ol: l 0. 7. 21.4. 42.8. 85 .6. l 00 UV treated Intake : l 00 C*riodaphniadubicr: Non-treat d utfall 101: I0.7._l.4.42.8. 5.6.100 Non-treated Intake: LOO
- 16. Permit Limi t Endpoint (%): utfall 101 : [ 2s = 42.8%
- 17. Test Re uhs: utfall l Ol : Pimephal s promela. : 1 2:- > l 00%
eriodaplmia dubia: IC2s > I 00% Page 1 of94
- 18. Facilit Contact : Millicent Garland Phone# : (423) 843-6714
- 19. onsulting / Testin 0 Lab : En ironm ntal Testin2. olutions. Inc.
- 20. ab nla t : Jim umner Ph ne #: (828) 350-9 64 Exp sure to sampl s collected May 1"' - 18. 2018 from utfall IO I re ulted in not xic effect to fathead mianow or daphnids. The resulting J 25 alues for both species. were > I 00 percent. E posure of minnows and daphnids to intake ample re ulted in no ignificant difference fr m tJ1e controls during thi ~ stud p riod .
Page 2 of94
METHOD UMMARY Samples: I. amp ling P int: utfall IO 1. Intak
- 2. ample Type: omposite
- 3. ample Infonnatjon:
Date Dat Date (MM-DD-VY) (MM-DD-VY) Initial (MM-00-YY ample Time ET) T) TRC
- T ime (ET lD ollected Lat Us dB 05-13-18 0755 t __ 9 05-15-18 11 05 101 05-14-18 1305 <0.10 05-14-18 0655 05-16-1 1008 05-] 3-1 0812 t Or-l;<:i-1 1105 lntak 05-i4-I I 05 2.2 <0. 10 05-14-18 0711 05-16-18 1008 05-15-18 0722 to 0 18 1013 LOI 05-16-18 1240 1.0 <0.10 05 1 0622 05-18-18 1037 05-15-18 0740to 05-17-18 I 1 Intake 05-16-18 1240 J. _ <O. L 05-16-18 0 40 0 18 l0"7 05-19-18 10 7 05-17-18 0657 to 101 0 18 13 I - 1.), l. 3t <0.10 05-20-18 os-1 s-18 o
- 1 s 05-21-18 05-19-1 05-17-18 0711 to Intak 05-18-18 1"'15 0.5 <0. 10 1 05-1 8- 18 0611 05-21-1
*TR = Total Residual Chlorine tsamples v ere collected in two 2. S gallon *ubitainers. Temperature was measured in each cub itain r upon arTival.
- 4. amp! Marupulation: . ample from utfall I 01 and intake,. ere warmed to test temperatur (25.0 +/- 1.0° ) in a warm water bath.
Y-treated tlu*ough a terilizer (manufactured bv mp ror Aquatics. lnc.) for 2 minute . Page 3 of94
Pimephales pr me/as eriodaphnia dub;r, I. ourc In-house Cultures Jn-hou Cultures
- 2. gc : < 24-hours old < 24-hours old est Method umrna1y:
- 1. Te t onditions: tatic. Renewal
- 2. Te t Durali n:
ontrol / Dilution at r: M derately Hard ynthetic ynthetic
- 4. umber Replicat : 4 lQ
- 5. rgani ms p r eplicat : lQ
- 6. est Initiation: (Date/Tim) : 05-15-18 1024
- T 05 18 I 105 ET
- 7. Te t Tennination: Oat Time): 05-22-18 0926 ET 05-22-18 1025 T
- 8. e t emperature: utfatl 1Ol : Mean= 24.6uc (24.2 - 25 . l )
9. I 0. Slati tics : Page 4 of94
ee Appendi, fi r Bench heet
- 1. Results of a PimephaLe promel 1s Chronic/ 7-day Toxicit Te t.
Genus specie ) (Type / Duration) Conducted May I - - 22.2018 u ing effluent rom utfall IO I . Te t Percent urv1vmg Solutions (lime interval used - days) (% Effluent) I 2 3 4 5 6 7 Control, I 100 100 100 10 100 JOO UY-treated 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 JOO 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 iOO JOO 98 98 Control, 100 I 0 1 100 100 10 Non-treated M a11 Dry Weight (mo) Te t Solutions (replicate number) (% EfOu nt) 1 2 3 4 Mean Control, 0.7 . . 0.688 0.614 0.65 0.680 UV-u*eatcd 10.7% 0.741 0.759 0.728 0.743 0.743 21.4% 0.728 0.743 0.719 0.750 0.735 42.8% 0.724 0.753 0.675 0.715 0.717 85 .6% 0.729 0.756 0.630 0.705 0.705 100.0% 0.705 0.690 0.731 0.694 0.705 Intake 0.628 0.568 0.608 0.677 0.620 ontroL 0.736 0.759 0. 44 0.602 0. 85 Non-treated fC2s Value: > I 00% Calculated U Estimat : < 1.0 TUc* Permit Limit: 2.8% Permit Limit: 2.3 TUc 95% Confidence Limit : Upper Limit: NA Lower Limit: NA
*TUa = 100/LCso: TUc = l 00/ IC2*
Page 5 of94
TOXI fTY TEST RESULT (see Appendix C for Bench h ets) Re ult fa Ceriodaphnia dubia Clu-onic/ 7-day Toxicity Te t. (Genu pecies) (T pe / Dura[j n onducted May 15 - 22. 201 using effluent fr m Outfall l 01. Percent urv1 m g Test (time interval used - days) I ulions l 2 4 5 6 7 (% Ef1luent) ontrol 100 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 JOO 100 85.6% 100 100 !Ov 100 ]00 100 100 100.0'Yo 100 100 100 100 100 100 100 Reproduction (#young/female/7 days) est olutions Data (replicate number) (%
- ff1uent) l 2 3 4 5 6 7 8 9 10 Mean ntrol 28 28 27 29 33 28 27 28 29 27 28.4 1 .7% ...... 32 _,_
1") . 31 l 31
- 32 29 29 33 29 31
.) .)
I,.- 21.4% 34 32 30 31 31 32 30 30 34 36 32.0
- 42. % 32 34 37 .......
32 35 37 34 30 .).) 31 33.5 85.6% 36 36 35 35 39 33 31 34 .,5 .,5 34.9 100.0% 37 33 37 39 36 39 36 34 34 37 36.2 I 1s Value: > 100% Calculated TU Estimate : < 1.0 TUc P rmit Limit: 42.8% Pennit Limit: 2.3 TUc 95% onlidence Limits: Upper Limit: _ Lower L imit: NA Ua = 1 0/L so: TU = lOO/ J s Page 6 of94
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day T xici!y Tes!.
(Genus species (T pe / Duration Conducted Mav 15 - 2 __ 2018 using water from Intake Percent urviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent.) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Repr duclion #y ung/lemalc/7 days) Te. t olulions Data r licate numberl (% Effluent) .., 2 4 5 7 9 JO Mean Control 30 29 26 30 0 29 31 27 30 2 29.0
..,., ., ') ..,.,
lntake 5 ..) 33 34 34 36 .)_ ..)..) 34 35 33 .9
!Cr Value: > 100% alculated T Estimate : < l.O Uc*
Permit L imit: N/A Permit Limit: N / 95 % Confidence Limit ~: Upper Limit: NA Lower Limit: A and D Duration 25 Pimephales promelas May08 - l5. 20 18 ors 7 day Ceriodaphnia dubia Ma 08 - l , 201 0911 7-day Page 7 of94
~
CQ for UV-11c.11ctl P1111cphc1l~.f prr,111c1c1s J1xl Nun-,r~;olcd < r11odopl1111a ,/11h1t1 5cq uo1ah Nuclcm l'i:11n (SQN), 1-fTiucnl O Ull:lll lO I ,mJ 1111.11.!' Cl) pcr~,m11.:d Mnv 15-22. 2018 Oo 0 I e~ 1 Sa mple 10 Tc mpcr:1 1111*c <"C)
-.. Diss olve d 0 X}l!CII (mt:/LJ pll (~.ti.) Conduc11111cc \lk" li nlty ll urdnch ' To tul ll cs idu:11 co lnit inl Fln:11 ln itlnl fi'i n:il l niti:11 Final (~unhos/c m) (mg/L CnCOJ) (mg/L CaCOJ) ('.blo1inc (mg/L)
~ ( OM l lfll , 2*1 7 24 S 78 7.J 7 95 7 68 307 (,1 83 -
N1111
- l ft' .tl tU 2-HI
- 24 8 24 3 - 24 6 7 6 - l!O uJ - !i *.1 7.85 - 801 7-1()
- 7.81 Jli(J
- 3 15 (10 61 82 - ~4 - .
rf) nuol. 24.7 24.4 79 75 7 98 7 74 309 61 !13
\ l\',,,u1frtl 24 <,
- 24 l! 24 J . N 6 76 - 8 I 6~ - Mll 7 91 - ~ l~l 7 5'l 785 1'}8
- 318 59 63 82 84 .
!:! 1(1.7%
2'1.8 24 .5 79 75 8 ()(J 7 (>8 ::!93 . - 24 7 - 24 S 24 J - 24 6 77 8. I 70 - 7'J 193 - ~ l/5 149
- 7 84 286
- JU() - - - .
~ 24 ll 2-1.5 so 7 .j 8 01 7.68 176 .
"'t 2lA%
147
- 24 9 24 2 . 24 7 77 . RI 69 - ~ l/ 1 <J~ - MO<, 75(1
- 7~5 2(,7
- 283 - -
.!!:: 1-1.8 2.J .-1 80 74 8.0 1 7 71 242 - .
-H .8'Ya
~
~
2n 24 9 2U
- 24 5 77
- 8I 6 () - 80 7 94 - 8.lJ<l 756 - 784 231
- 246 . . -
-:::. 24 .9 2,1 3 80 7.4 7 99 7 62 17!1 - . -
c:.. 85 .6% 24 8 - 24.9 24.2 - ~4 6 7 S - X3 bM 8 () 7 1!1
- 805 7 45
- 7 76 17 1 187 . . .
N9 24 4 8I 74 7.9S 7.61 154 :i8 63 < 0. 10 IOU% 248 25 0 24..2 - 24 6 77 - 8.3 b7
- 8I 7 91
- 8 0-! 7 1 7J 146 161 54 62 00 6-1 < U 10 - <O 10 25 0 24 .5 II. I 7-1 7()8 7.60 154 56 GO < 0 10 lninlu, 24 9 - 25 I 24 2 24 7 78
- 8.J 6\J 81 7 8')
- 8 ().4 731 779 143
- lb l 56 56 6<) . 60 <U 10 - < O 10 l
- 11 11111. 24 7 25. I 78 79 7 9S 8.00 J07 61 !I] -
60 . 6 1
- \ ~n .. 1n.* .,lr1I 14 6 - 248 24 CJ . 2..i2 76
- 80 77 . 8.l) 7X5
- 8l>'l 792 - 8 ()..I JtKI
- JIS 82 84 2H 24 .9 79 7 () 7 99 8.00 287 .
I0.7 % 24 7 . 24 9 24 8
- 2.5 2 7 8 - ISO 7.8 - 8.U 7\ll - 807 792
- 805 1811
- 294 . . . -
,::. uo .
2 1A% 24 .8 25.0 79 79 8 8.00 173 -
-:: 24.7
- 24.9 248 . 250 77 - 8 I 7.8
- 8.0 7 91
- 8 07 792 - lW3 265 282
*~ 2-1.l! 24 9 7.9 7 CJ 8 00 7 99 24 1 . . .
~ -12.8%
$- 24 7
- 24 '} 24 8
- 25 I 7 7 - I! I 7.8
- 8 i) 79J 8 07 7 'll
- 8 OJ 237
- 25(1 . . .
~ .
1:: 85.6% 24 .9 24 9 79 79 7.98 7.97 .11<i - (i 247
- 250 24 8 - 25 2 77 - II.I 7.8
- 8 \I 789 - 806 78Q - 802 171 17<> - - .
IOU% 24 .9 14 9 80 7.9 7 911 7 95 Ii I 58 <,-1 < 0 10 248 - 250 24 7 - 25 I 78 . IU 78 * ~! 7 88 - 811-1 7 81!
- M00 138 - 156 58 . "8 (iO . (,lj < ll 10 - < 0 JO 24.9 24 8 8.0 8.0 7.97 7 97 154 57 61 < O 10 lrllu kc 24 7 - 15.0 24 8 - 25.0 H- 8.2 78 - 8I 71)1 tOJ 7 88 - S (),I 147 15') 56 . 58 (,{) 64 <0.10 * < O iU pc ~
Ovcrn ll lc 111pc 111 1nrc (°C'l vc ra~e ~l inimu111 1\ l :t~im11 m
/'11111:p/111/,*.r promela.1 24 <, 24.1 25 I I ... ,.,,,d,111(111111 d11h,n 24 ,, 24 f, '.!5 2
UMMARY / CON L SIONS Expo urest amplescolle led Ma 13 - t8 201 fr m utfall 101 r sulled in no t xic effe ts to fathead minnows or daplmid . The re ulting [ 25 alu s. tor both species. ¥ere> I 00 percent. Exposure of minn ws and daphnids t intak sample re ulted inn significant differ nc fro m the conlrols during thi stud y period.
ppcndix A DDITIO A TO f ITY TE T INFORM TIO I . Pimephales prome/as e t were conducted a cording to EPA-82l -R-02-01., October 20 2 using fi ur replicate , ea h con taining ten te t organisms. p r treatm nl. Test vess ls consisted of 500-mL pla tic di po ab l up . each containing 250-mL of test solution.
- 2. eriodaplmia dubia Tests wer conducted according to EPJ\-821-R-02-013 October _002 using ten repljcat ac h contai ning 011 test organi sm, per treatment. e t ves els onsi ted of 30-mL p lypr pylen cup . each containing 15-mL of test solution .
- 1. Pim ?pha/es promela ample used in th fathead minn w test were exposed to UV light for two minut s p1ior to introduction of te ' t organi ms. UV treatment is used to ontrol interference of ti h pathogen . Thi treatment meth d was approved n November 23 _Ql5 by the State of T 1messee in a !en r fr m J sica !urphy to Terr h ek. enior Manager f TV Water P rmit. ompli ance. and Monitoring.
- 2. eriodaplmia dubia N n J . Pimephale promela ne
- 2. eriodaphnia dubia one Page 10 of94
- 1. Reagents, Titrants. Buffers, et .: All chemical were certified products used b for expirati n date (where applicable) .
- 2. In trument : All identification, service. and calibration infonnation pertaining to laborator in trument is recorded in calibration and main enan logbooks.
- 3. Temperatur was mea ured by M 2550 B-2010.
- 4. Di solved xygen wa measured by SM 4500-0 G-20 I l.
- 5. The pH was mea ured by SM 4 00-H+ B-201 I.
- 6. Conductance wa measured by M -510 B-2011 .
- 7. Alkalinit was mea ured b M 2 20 B-2011.
- 8. Total hardnes wa m asured by M 2"'40 C-2 11 .
Total residual chlorine wa measured by RI N 7-7 -1 77. 0 E Toxicit Te l M th d : AJJ phase of the stud including, but not limited to, sample collection, handling and storage, gla ware pr paration, test organism culturing/acquisition and acclimation, te
- t r~ani *m ha:1dJing during te L, and maintaining appropriate te t conditi ns were conducted ac ording t the pr t c l as described in this report and EPA-S21 -R-02-0] 3. 1 known d<;:viations,,; ere noted duri11g the tudy and ar rep rted herein.
REFEREN ndix D for ontrol chart infonnati n I . Test T pe: 7-da , clu* nic te ts with results *pre d as I r valu in g/L K I or a 1.
- 2. tandard T icant: Potassium hlorid (K I cry talline) for Pim '}Jhales promelas.
dium Chlorid Na I ry tallin for Ceriodaphnia dubi t.
- 3. Dilution Water Us d: Moderat ly hard syntheti water.
- 4. lalisti s: To ale oft via.re Version - .0 wa u ed for tatisticaJ anal e .
Page 11 of 94
I. PDE Permit o. TN0026450. U PA.
- 3. tandard Methods for the Examination of Water and Wast water. 2_nd Edition. 2012.
- 4. peratine.. ProcedlU"e , En ironmental T sting Page 12 of94
equ ah Nuclear Plant Biomonitorino May 15 - 22. 20 I 8 pp ndix B Diffu r Dischar e oncentrations of Total Re idual Chi rm , 0 Diffuser Discharg n ntration f Ch mi als ed t Contr l Mi robio logicall lnduced CotT sion and M llu k During To. icity Te t ampling Page 13 of94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-lSOM Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat TRC EO/PO 02/06/2005 - <0.0042 0.028 0.010 - - - - - 02/07/2005 - <0.0116 0.028 0.010 - - - 0.007 - 02/08/2005 - <0.0080 0.028 0.010 - - - - - 02/09/2005 - 0.0199 0.028 0.010 - - - - - 02/10/2005 - <0.0042 0.028 0.010 - - - - - 02/11/2005 - 0.0155 0.028 0.010 - - - 0.007 - 06/05/2005 - 0.0063 - - - - - - - 06/06/2005 - 0.0043 - - - - - - 0.037 06/07/2005 - 0.0103 - - - - - - 0.037 06/08/2005 - 0.0295 - - - a - - 0.037 06/09/2005 - 0.0129 - - - - - - - 06/10/2005 - 0.0184 - - - - - - - 07/17/2005 - 0.0109 0.026 0.009 - - - - - 07/18/2005 - 0.0150 0.026 0.009 - - - - 0.036 07/19/2005 - 0.0163 0.026 0.009 - - - - 0.036 07/20/2005 - 0.0209 0.026 0.009 - - - 0.014 0.036 07/21/2005 - 0.0242 0.026 0.009 - - - - - 07/22/2005 - 0.0238 0.054 O.Q18 - - - 0.014 - 10/30/2005 - 0.0068 - - - - - - - 10/31/2005 - 0.0112 - - - - - - - 11/01 /2005 - 0.0104 - - - - - - 0.035 11/02/2005 - 0.0104 - - - - - - 0.036 11/03/2005 - 0.0117 - - - - - - 0.036 11/04/2005 - 0.0165 - - - - - - 0.035 11/14/2005 - 0.0274 - - - - - - - 11/15/2005 - 0.0256 - - - - - - - 11/16/2005 - 0.0234 - - - - - - - 11/17/2005 - 0.0231 - - - - - - - 11/18/2005 - 0.0200 - - - - - - - 11/19/2005 - 0.0116 - - - - - - - Page 14 of94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-ISOM MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11/12/2006 - 0.0055 - - - - - - - - 11/13/2006 - 0.0068 - - - - - - 0.037 - 11/14/2006 - 0.0143 - - - - - - 0.037 - 11/15/2006 - 0.0068 - - - - - - 0.037 - 11/16/2006 - 0.0267 - - - - - - 0.037 - 11/17/2006 - 0.0222 - - - - - - - - 11/26/2006 - 0.0188 - - - - - - - - 11/27/2006 - 0.0138 - - - - - - - - 11/28/2006 - 0.0120 - - - - - - - - 11/29/2006 - 0.0288 - - - - - - - - 11/30/2006 - 0.0376 - - - - - - - - 12/01/2006 - 0.0187 - - - - - - - - 05/28/07 - - - - - - - - - 0.015 05/29/07 - - - - - - - - 0.036 o.oi5 05/30/07 - 0.0084 - - - - - 0.017 0.036 o.oi5 05/31/07 - 0.0103 - - - - - - 0.036 0.015 06/01 /07 - 0.0164 - - - - - 0.017 0.036 o.oi5 06/02/07 - 0.0305 - - - - - - - o.oi5 12/02/07 - 0.0241 - - - - - - - - 12/03/07 - 0.0128 - - - - - - - - 12/04/07 - 0.0238 - - - - - - - - 12/05/07 - 0.0158 - - - - - - - - 12/06/07 - 0.0162 - - - - - - - - 12/07/07 - 0.0175 - - - - - - - 04/13/08 - 0.0039 - - - - - - - - 04/14/08 - 0.0124 - - - - - - - - 04/15/08 - 0.0229 - - - - - - - - 04/16/08 - 0.0143 - - - - - - - - 04/17/08 - 0.0120 - - - - - - - - 04/18/08 - 0.0149 - - - - - - - - 10/26/08 - 0.0260 - - - - - - - - 10/27/08 - 0.0151 - - - - - 0.017 - - 10/28/08 - 0.0172 - - - - - - 0.041 - 10/29/08 - 0.0154 - - - - - 0.018 0.041 0.030 10/30/08 - - - - - - - - 0.041 0.030 10/3 1/08 - 0.0086 - - - - - - 0.041 0.030 Page 15 of 94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 18, 2018 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101" mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 - 0.0197 - - - - - 0.017 - - - 02/09/09 - 0.0237 - - - - - 0.017 - - - 02/10/09 - 0.0104 - - - - - 0.021 - - - 02/11/09 - 0.0155 - - - - - 0.017 - - - 02/12/09 - 0.0106 - - - - - 0.017 - - - 02/13/09 - - - - - - - - - - - 05/10/09 - 0.0129 - - - - - - - - - 05/11/09 - 0.0415 - - - - - - - 0.0446 - 05/12/09 - 0.0053 - - - - - - - 0.0396 - 05/13/09 - 0.0049 - - - - - - - 0.0396 - 05/14/09 - <0.0141 - - - - - - - 0.0397 - 05/15/09 - <0.0160 - - - - - - - - - 11/15/09 - 0.025 - - - - - - - - - 11/16/09 - 0.0152 - - - - - - - - - 11/17/09 - 0.0255 - - - - - - - - - 11/18/09 - 0.0306 - - - - - - - - - 11/19/09 - 0.0204 - - - - - - - - 11/20/09 - 0.0093 - - - - - - - - - 05/09/1 0 - 0.0192 - - - - - - - - - 05/10/1 0 - 0.0055 - - - - - - - - - 05/11/10 - 0.0100 - - - - - - 0.039 - - 05/12/10 - 0.0171 - - - - - - 0.039 - - 05/13/10 - 0.0041 - - - - - 0.039 - - 05/14/10 - 0.0099 - - - - - - 0.039 - - Page 16 of94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EOIPO Quat Phos- Phosphate TRC J>hate ohate 10/31/10 - - - - - - - - - - - - 11/01/10 - 0.0122 - - - - - - - - - - 11/02/10 - 0.0112 - - - - - - - - - - 11 /03/1 0 - 0.0163 - - - - - - - - - - 11/04/10 - 0.0107 - - - - - - - - - - 11/05/10 - 0.0132 - - - - - - - - - - 05/01/2011 - - - - - - - - - - - - 05/02/2011 - - - - - - - - 0.04 - - - 05/03/2011 - - - - - - - - 0.04 - - - 05/04/2011 - 0.0155 - - - - - - 0.04 - - - 05/05/2011 - 0.0179 - - - - - - 0.04 - - - 05/06/2011 - 0.0089 - - - - - - - - - - 11/06/2011 - 0.0168 - - - - - - - - - - 11/07/2011 - 0.0225 - - - - - - - - - - 11/08/2011 - 0.0141 - - - - - - - - - - 11/09/2011 - 0.0239 - - - - - - - - - - 11/10/2011 - 0.0242 - - - - - - - - - - 11/11/2011 - 0.0231 - - - - - - - - - - 05/06/2012 - - - - - - - - - - - - 05/07/2012 - - - - - - - - - - - - 05/08/2012 - - - - - - - - 0.041 - - - 05/09/2012 - 0.0145 - - - - - - 0.041 - - - 05/10/2012 - 0.0298 - - - - - - 0.041 - - - 05/11/2012 - 0.0174 - - - - - - - - - - 08/12/2012 - - - - - - - - - - - 0.029 08/13/2012 - 0.0256 - - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 - - - - - - 0.037 - - 0.029 08/15/2012 - 0.0279 - - - - - 0.028 - - - 0.029 08/16/2012 - 0.0076 - - - - - - - - - 0.029 08/ 17/2012 - 0.0446 - - - - - - - - - 0.032 05/12/2013 - 0.0099 - - - - - - - - - - 05/13/2013 - - - - - - - - - - 0.064 05/ 14/2013 - 0.0091 - - - - - 0.039 - - - 0.064 05/15/2013 - 0.0096 - - - - - 0.039 - - - 0.064 05/ 16/2013 - 0.0229 - - - - - - - - - 0.032 05/17/2013 - 0.0063 - - - - - - - - - 0.032 09/15/2013 - - - - - - - - - - - 0.03 09/16/2013 - 0.0072 - - - - - - 0.0379 - - 0.03 09/17/2013 - 0.0107 - - - - - 0.036 0.0379 - - 0.03 09/18/2013 - 0.0217 - - - - - 0.036 0.0379 - - 0.03 09/19/2013 - 0.0172 - - - - - - - - - 0.03 09/20/2013 - 0.0173 - - - - - - - - - 0.03 Page 17 of 94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PFmg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD
- Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos- mer EO/PO Quat Phos- Phosphate TRC obate obate 05/04/2014 - 0.0118 - - - - - - - - - -
05/05/2014 - 0.0112 - - - - - - - - - - 05/06/2014 - 0.0096 - - - - - - - - - - 05/07/2014 - 0.0164 - - - - - - - - - - 05/08/2014 - 0.0235 - - - - - - - - - - 05/09/2014 - 0.0110 - - - - - - - - - - 09/07/2014 - - - - - - - - - - - - 09/08/2014 - - - - - - - - 0.04 - - - 09/09/2014 - - - - - - - - 0.04 - - - 09/1 0/2014 - - - - - - - - 0.04 - - - 09/11/2014 - 0.0070 - - - - - - - - - - 09/12/2014 - 0.0074 - - - - - - - - - . 08/09/2015 - - - 08/10/2015 0.0195 0.03 - 08/1 1/2015 0.0275 0.03 - 08/12/2015 0.0213 - O.o3 08/13/2015 0.0192 - 0.03 08/14/2015 0.0182 - 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 O.o174 10/23/2015 0.0156 05/ 15/2016 - 05/16/2016 0.0209 05/ 17/2016 0.0210 05/18/2016 0.0361 05/19/2016 0.0254 05/20/2016 0.0261 07/31/2016 - - 08/01/2016 0.0091 0.03 08/02/2016 0.0093 0.03 08/03/2016 0.0209 0.03 08/04/2016 - - 08/05/2016 - - 04/30/2017 - 05/01/2017 0.0298 05/02/2017 0.0218 05/03/2017 0.0260 05/04/2017 - 05/05/2017 - Page 18 of94
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 18, 2018 Date Towerbrom Floguard Spectrus BD1500 Nalco Spectrus Floguard mg/L MS 6237 mg/L 73551 CT1300 MS6236 TRC mg/L Quat mg/L mg/L mg/L Phosphate/Zinc EO/PO Quat Phosphate 07/23/2017 - - - 07/24/2017 0.0124 0.04/0.01 - 07/25/2017 0.0081 0.04/0.01 - 07/26/2017 0.0232 0.04/0.01 0.03 07/27/2017 0.0179 - - 07/28/2017 0.0296 - 0.03 5/13/2018 5/14/2018 5/15/2018 0.0229 0.030 0.0275 5/16/2018 0.0159 0.030 0.0275 5/17/2018 0.0133 0.030 0.0275 5/18/2018 Page 19 of94
quo ah uclear Plant Biomonitoring Ma 15 - 22, 2018 Ap endix C barn of Custody Records and Toxicity st Bench She ts Page 20 of94
BIOMONITORING HAIN OF CU TOD\' RE ORD Pag 1 of 1 Cl~ t: TVA En ironmental Testing Solution In. Delivered By ircle One*
~
Pr'1Ject Name: equoyah NP Toxicity 351 Depot Street. Fed x UP Bus Client h:l, P. O. Numb r. NIA Asheville C Other specify): So("\~(!_ 'De It"t, .... ru Fathity Sampled: Sequoyah NP ' 28801 G n ral Comment : 11,,X, .5o.,..p(-eS 7NA' 5a ... p>>!. I tro~ IOI S~\-.,(2 t>'lf/1 5*t~,/9' tro- ~-\. ~pier@ /)825 r, ... S'*P/-/? t>n Ph ne: 28-350-9364 PD 1 Colli d By*~
~
umber: TN0026450
.PA-/\IT6fl,1 ~ s ~-~
Lr.I<-//~ :.7"_,., ~ Fa 28-350-9368 ' IOI o,ac~ ~le. wo.1 ,~t~ t V1'$S /l'fd4!.! l,,J<:rc Oal/, o-./t!.c/ + ~pf
~ ~ec p,i s , i!~
Field I dei1't il cati n / ..._ rcfrnb~ ~lleC!i n Date/Time ample D criptlon ontoiner Number& low (MOD) Rain vent? PM'~ \333i Laboratory Use Volume (Mark as Appr priale Collecced V lfYcs. No T ETSl~g Arrival Temp B Time Appear*
- tart End
-- *- In hes umbtr <* ~
ancc
-101-TOX Comp ~-13 -t F b '155 Er S'-1'1- 1?
!)~55 ~,. I
.-A...11
.Z(-. gal) libS. oo X leo51"i-02.. 2 -~ - c_ 6' tpS SQN-INT-TO C mp S
- l3*1J>
of 12. £1 5"*/1/* 1? {) 712. ~ '- I (2. gal) N x_ tt30S11.{ . o:!) '2.. .1. . (_
! \~O~ -j.
Sample Custody Fill fn From Top Down -'J. (_\I~~--\ ~ A l c;. \ ~ , . 5f\t1.ILCS lu..l.e.\\J ~t) l~ 6-aob c.,r~\;:t\CN . ~ Relinqui hed B ignature ; I 1me Recei ed B ( ignature at 1me
\~~
AL_fl~ 5-Jt/,J? /o?S8'Ei '~ /(. . 9_~ /)_ -
~i->\(.
~-1,J~
os--J~<.;1t t;t'o,~YE"t J~ V
$ow\(_
Q£ g,_- \'n~..., b J'-1 Y-1f'/ L) ; ()1- ~ ,
~f( /v----_ E"'\S os-,'4-,~ \~OS £X V I.,
In 1ru lmn
- Ii n~ houl fill m II a cep1 t em e *= *-.;-1 l) e~ I c Biomonil nng mpl re pre c d nn 1h m 1 "C J ,ppin th m m ice. The h ld um ~ r *h _ ,~
,* 6h urs fr rn 1ht ume c II* uon. Thercl re, pica oll and IP m SU h a \ a ' th I lhc I or ory will rec I c th mplc \\ith pl timet 1nttint testing, ithin lhal time fram
- ample hipp (1 rmght on rid 111a FedF. ur lJP mu th m rked ~ r . rurda dcl e or the. , ill no1 arrive until the follm mg Monda}
I Page u, \ Whole Effluent Sample Receipt Log
- Sample temper~ture performed using Sample Rcttiving Thermometer. SN 160928622 Date Time Receiv d Received
- Sample Project Sample 0
Sample name and description State Comments Received Received by from Temp. ( C) number number 05-14-18 0906 K. Keenan Fed - Ex NA 13336 180514 .01 CORTROL IS3020 TN 05-14*18
- 1305 J. Sumner TVACourler 2.9 13337 180514 .02 TVA - Sequoyah Nuclear Plant-* 101 TN 05-14 -18 1305 J. Sumner TVA Courier 2.2 13337 180514 .03 TVA* Sequoyah Nuclear Plant
- lncake TN SOP G4
- Exhibit G4.2, revision 01-0.3-12
Bl MONIT RIN H I OF T DY R I ORD Pa~c _l_ uf _l_ CQ
~
Cl) licm: TVA Environmental Testing olution Inc. Delivered By ( ircle II*): c:l Project amc: Sequoyah P Toxicil) I D pot Street. 1:cdl~: UP Bu ClieH 0.,, t l'.0. Number: NIA Asheville N Olhcr (sped y): 5onis;.. De. Ji'~~,-,1/ 2 801 G ncral Co111mcn1s. Fncih1y ampled* *equ y. h p Tcok 5o.-pte.s .fro-, tot >?l-er~07..J7 "" S-:/,*J. Ph nc: 828-3 0-9364 Too 1: ~pt~.s .fto'"' r...s-r ~-ptu@... 1)7~/ o"' .s-l&-1? NPDFS um er* TN002645
/
~.,ore:.- 'lt-101 Ba.cl:.o.~ ~plc:1 1,Jc..;:, ll<<:f (5(\. ~;~c:
Collccr d Oy: ~ ~,.J~t.).,l,J!
* , . . ~ r e 1 c S t)
*,.-£?.); v---
~Fax: 28-35 -936
;/,(. Diti~ . ,vt~cil!> IA .c (2..of(-cd--c.J 't- ¥-~*I l>Y':!.i
*1dd IJ.:otilio111on I ,r:i Comp
- n1mncr 110,, (333+
- nmrlc: Oc.scription C llcction Date 'imc Number& (MOD R.in l::wnt1 ~00j e.t.f l.:1hor:llo lJ~c Volume IM:u : , /\pl)fl1prit11c)
L,1llcLtc,I Yes If Yo*. Nn Trnc~ ITTS Ln~ ,\rrival *t cmp Oy Tim" .\pre.a,
- - -* lf\Che~ N11111hcr c*c-1 ,*ni.*
St, n End
;tt-o't.1-..,1Y E...-
SQN- 101-TOX Comp s--15 .. 11? 5-1~-l'lf ...212 5g I ({)nl\4.6LI \.O
- r..._ \1.-10 j t>?ZZ £ r ObZZ. E1 1- l'ltl}.7bi X -~
s-1s -1'!{ S*lfo~IK SQN-INT-TOX Comp 011/IJ ;f1 !){.pt/() E. I.. I (2 5 gal) NA i \&6\~.oS \ .1. c.. o\ "1.'10 t Sample usiody - Fill In From rop Down -'lf C.v~,o~ ~V\\,J /~,~LT. <;.AHf\.e.<:; Relinq111shed B (Signature): D tc/Time Rec.?i *i.:d B
- Signature);
~K.e.<J 1ve.D \w G-<>t>fiill"&'i-~ Q\TlOIJ.
11ne ()
/?zo. /v/___ ,.J~ 5*/b-!f/ !J?I 7 e:- 13 R. 31!~ Sti~,c..
"°' '-le.a."\ ds -/' ,./( ~
/
~!') ET' 531~ 5-)w c..
be.\..\-.i&.-l cJ b--16.'t ,I J. yo ~ fl'-}v
' V
- £~ Oi*lt,*tf l?..'10 e, Ir st ru,11
- C'ltCIII ll'Uld ill II"' ,. cert ch ,c an 1h "I. h 1.,tn II :: 1111 *l flu111 11111rini; .11n11k 1*1 *. *n J h tt r111
- t cm al 6' * .111 *t,iripot
- ah 11110 n,~ h 1111i . r," m *1 '
,. 1ft h,.,., Ju 01 ll ~ 1n,
- f I '(I: Ill<' (lk,I' 1,IJ *1 :mJ h rt 111 11 h "l. lh.,1 lh
- IJl 1\1 \ H ,1;"'- *t 11 * ,mi,k , *uh, 11,pk tnn 1n 1n,11. \ 1 IIO nth* lh I Inn.: fr:11
- c<lf::- l*r "P!'.
r '"' ,111 ,, ' I 11\ :i*H;! I lm f.ri.la~ "-' 11111 t ~ 1na1 , d 11 r *u1urd,1
- d,:l1vc 111 th *1 will n,11 :1rm c until th* folln11111
- II 11'111:1)*
-1 Whole Effluent Sample Receipt Log Page~
- sample temperature performeo using Sample Rece,v ng Th ermometer: N 160928622 Date Time Received Received Sample Project Sample 0
Sample name and description State Comments Received Received _by from Temp. ( C) number number 05-16*18 0827 K. Keena n 5. Gouge 3.1 13340 180516 .01 Baxter Healthcare NC 05-16-18 0957 K. Keenan Fed - Ex 2.3 13341 180516 .oi Enfield WWTP NC 05-16-18 0957 t<. Keenan Fed
- Ex 2.3 13342 180516 .03 Scotland Neck WWTP NC 05-16-18 1240 J. Sumner TVA Courier 1.0 13337 180516 .04 TVA* Sequoyah Nuclear Plant -101 TN 05-16-18 1240 J. Sumner TVA Courier 1.2 13337 180516 .OS TVA - Sequoyah Nuclear Plant - Intake TN SOP G4
- E><hlbit G4 .2, revision 01-03-12
BlOMONITORJNG CHAIN OF CUSTODY RECORD Page _ l _ of _ 1_
~.
CINtft: TVA Environmental Testing Solution, Inc . Delivered By (Circle One): Pr$ct Name: Sequoyah NP To icity 351 Depot Street. FedE UP Bus Client
~-
P.:Number: NIA Asheville, NC Other (sp cify : I) ,. \ ,\;e f
.... """' I"\ ; .-,
I co 28801 General Comments: f", g FadMity Sampled: equoyah NP too~ ~-pi-es tfo,.., lo\ 5... ""'~l<re ()~5D 6 " ,Jg-I Phone: 828-350-9364 -r.,., K ~pl-t>S -fro-. "I:...i. . Sa.1--t~ler@ 01Jt/ t>" 5-18-tJ Byd. NPO Number: TN0026450 Collected tJ. MltJrt:... Fax: 828-3 50-93 68 _o4 *b.-s.s. Me.h~~s ~u~ eollec~.J t ~4+ O'f"' ~;~ Field Identification / Grab/ omp - ollection Date/fime ontainer Flow prw}vt~ \3~3'1-mple Description Number& (MGD) Rain Event?
- Laboratory Use Volume {Mark as Appropriate)
Collected
- tart End
-- - Yes If Yes, Inches No Trace ETS Log Number Arrival Temp.
{°C) By Time
~
Appear-ance QN-10'1-TOX Comp S-17* 1? 0&57 Er S"- IS'-li' PSS7ET" 2 {2.Sgal 1'11-l. 41 X 1oo;,a.o3 \.\ '\.~-(_ o' \'?,\"1 t Comp 6- 17*/i 5-/1- tY \8th18 .bY SQN-£NT-TOX NA
;(
I (2.5 gal)
\~\<,
(}'7// £1 /)(pj/ a- Q .~*L
~
Sample Custody - Fi ll In From Top Down ~tv~ ~ l~T , .C .A.-1~ ~!tt.\\)j C) Relinq uished By.,,,(Signature): Date ime Received By (Signature): t,.) G-oo t t.ci ~b,-no),,J. ohJT* e 1me /tL {)d ,.J~ { ~tr tt oi1sE~I B1<~ S6-->,c.. l:::R.L,~ut.~ o s-;.. f <il-1r;rI O</.
- is----PS A~ 21~ jffei
{./ So~,l I (
))e~-.\ ~ s-/9,-ft? /tJ.1j .. ,~ (......._,, l\w,.... / t~ O~*\f
- I~ \~\':> ~
/
In trucli ns: Cl ients hould fill in all area except thos in the ul.ab rato * ,. bl k. Biomonitoring samples are preserved b taring them at °C od hippin them in ice. The hold ti111e for each s mplc i hours from the time of collection Therefore, pie e c Hect and hip in su h a , that the laboratory will receive the . amples with ample time 10 ini1 ia1 te. ting, ithin that time frame. amples shipped 11em1ghl on Friday via Fed ~x or P. must e marked for aturda. d Ii e r th y will not arrive unti l Lhe following M nda .
I
\
Whole Effluent Sample Receipt log Page t..S
*sample 1en1peranJre performed using Sample Receiving Thl!fmomete . SN 160928622 Date Time Received Received *sample Project Sample 0
Sample name and description tate Comments Received Received by from Temp . ( C} nu mb er number 05-18-18 0948 J, Sumner Fed - Ex 2.1 13341 180518 .01 Enfield WWTP NC OS-18-18 0948 J. Sumner Fed
- Ex u 13342 180518 .02 Scotland Neck WWTP NC 05-18-18 1315 J. Sumner TVA Courier 1,1/1.3 !3337 180518 .03 TVA
- Sequoyah Nuclear Plant
- 101 TN 05* 18-18 1315 J. Sumner TVA Courier 0.5 13337 1aos18 .o~ TVA* Sequoyah Nuclear Plant - Intake TN SOP G4
- E><hibit G4.2, revision 01-03-12
Page Io 7 I Chronic W hole Effluen t oxicity Te *t ( 1 P -821-R-02-013 Method 1000. Sp de : Pimepll ales promelas 1 ou nty; Hamilton Outfall: 101 Project#: _ _ , -~-;':)_:-S_.\ 1 Dilution preparation i11(ormnlio11: Dilution prep (%} 10.7 21.4 42.8 85.6 100 Co111111e11ts: Each conce,iu-ation was UV-treated Effiuc111 volume (111L) 267.S 535 1070 2140 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2232.5 1965 1430 360 0 Interferences . Total volume (mL) 2500 2500 2500 2500 2500 Test orgllllism i11fo rmalio11: Test in(or111ntio11: Org,anism source: In-house culture Random izing templale: LllrH\" 0 ~ Age: < 24-hou rs old Incubator number and
\~
she! f location: Spawn date: a::,.00..-\& Anemia CHM number: CHM984 OS*\~-\ i ,,;."\..S "Cl Hatch dates and times: DIJ in<> i11/or111atio11 for weioht (\~-\C-,, *\& o<;OS Transfer vessel infonnation: pH=
,_.,, s.
deter111i11r11io11: Date/ Time in oven : OS,l.."l..*\k 00\~ Temperature - ,_-\.°':) oc Initial oven temperature: l.ro ' L Average transfer volume: <0.25 mL Date/ Time Ollt of oven: 0S*1:~*l.R (Qoo Final oven temperature: l,,()" c..... Total drying time: "1.'.\ o..>D~ Daily feeding mul reuewnl i11/or111atio11: D:iy Dntc I Morning feeding Aftcmoo11 r;:c<lin" Tc t initintion, rcncwnl, or Snmplc numbers u cd MllSW hntch tcrrninntion used Time Arrnlyst Time Annlyst Time Annlys t Outfall IO l Intake 0 05-15-18 hc ,o )'(
\t..\O u. \ 0-8 vi \.g os.H. . n_ li oS \'-\ . 03) oswtR A I 05-16-18 ,..~,~ \I \1. l~ \,( oC\"\..S
2 * ;, 05-17-18 CJ'\..\1.-
..ll o"" le os,1c. .n<;;
3 4 05-18-18 (JI~() en'-\\ vi u*
\,f u
l:>~Q
\~~~ ~-
\1 OC\S'c ,.,t 1&0S 11.. .
l .'-1:61\o- ()~ \iU';llo. ~ o~- 1l.t~ II l(\ C:. *\ Ir:,* t.f p.. 05 18 ~'-\.(.,. l,t I ; -.\<; l/ D0.S""\ "~ lRoSti-0~ If Cl c; lf-0 --\ 0~*11,,- 1i A u* 5 05-20-18 CrOO \( \'\OO IA 1nl\ ..\A 1 1- t\S \8' , If\ ,czos1c-o-t ~-\\,
- lfA.
6 05-21 18 Ol.H.) ).\ u*
\ 1..t I,) ,.,,...
I\ of.\ '2.S M li O.i le , (f!) l.foS tf -O"l o~-11.*u I'\ 7 05-22-18
\J.
oo.1 -lo *~ Co11trol i11formatio11: l)\J. c..n '-'1""tO I.... Acccprnoce criteria St1111111nry of test e11tlpoi11ts:
% Mortality: () 7. s20% 7-day LCso '> 1001 Average weight per initial larvae: 0.1.,go NOEC 100 7.
Average weight per surviving larvae: O . l!>fO >- 0.25nig,'larvae LOEC >1007. ChV ) 1007 ICzs ~1007. Page 27of94 SOP AT20 - Exhibit AT20 .3, revision l l-01-14
~ ~ -
Page 2 of7 Date: 05-15-18 urvivnl nud Growth Dntn Da CONTROL 10.7% 21.4% A B C D E F G H I J K L 0 10 10 10 10 10 10 10 JO 10 10 10 10 I 10 10 IC) l () ll) IC) ID ID 10 I l) I () ro 2 ID It) l t) 10 IC) lO 10 IC) IC) l~ l () l~ 3 I t) ID ID lU IO ( t) IC) IC) ID ( \J lO I() 4
't, lQ I () 10 \0 10 lO 10 I () 10 ro to 5
ID ,o 10 10 IC) \b \Q I lJ ID IQ l~ 10 6 I (.) 1-0 l \) ID lU l C) LC) LO lO lO l D LO. 7 ID ,a / t) I0 'D ro 'D /Q /0 tO lQ LO
= P:m wcigh1 (mg)
Trny colo r code:: ~(~ nnlyst: TS /5. t.f l 1a.1i /5,5.2. Ho.13 15.~0 /{o,1~ 15.St \3.04 l'i. IC\ /fl.OJ.. Jt~5 15 14 Dnlc: Q~ -Ql- l i ' o- P:111 + Lnrvnc weight (mg)
/\ n:tly t: i{ ,2. . l\ t f.'lb 2.? .a; 'l.\.16 "?.'2,.1.,l -Z.,j ,'?)~ -i1..,'\ 20,<41 2 1.<J7 -z.~."s -z.1.~I 2:z.1..\.I D:ilc: O'i*t3* ,g_
J C = L:1 rv:ie wet ht (mg)* B - A 11:tntl c:11 olnted. Anni *st: t l.,.R&
- __os. ~
!.. .S~
, .1.~ , .*t\ ,.~'\ , . 'LB .. ~ ,.u , . . ,~ ,. '°' ,.so I
(. ,
\Vcighl per initi:tl number oflnrvnc (111r1) = / lniti. I number of lnrvn~
Homl cnlculntcl.l. Cl} ..}
~
'V:p ~
N; o"'*
~' o*"~ "-
"'q. ~~
o*"'
~
A. o* o*
~ /\\.'>\
o* A"::,0 j nntysr: el o*"' o* o* o* o* o* Avcr:iuc wcigh 1 per Percent rccluction initinl number of lnrwtc(mg) from contr*ol (%) D.lo &b O .i~ - °'.-~1. o.,oS - g. 1. 7. Comment codes: c = clear, d = dead, fg = fungus, k = killed, rn = missing, sk = sick, sm = unusually small, lg= unusually large, d&r = decanted and rerurned , w = wounded. Comments: Page 28 of94 SOP AT20 - Exhibit AT20.3revision11-01-14
Pag 3 of7 Species: Pimeplwles promelas Client: TVA/ Scguovah Nuclear Plant, Outfall 101, UV-treated Date: 05-15-18 Survival anti Growth Data Day 42.8% 85.6% JOO% M N 0 p Q R s T u V w X 0 10 10 10 10 10 10 10 10 10 10 10 10 I 10 10 IO co >o IO IQ C0 ID ID 10 ID 2 tD It> co IC) LO l C) l~ /Q It) l t\ ta ID 3 tD 1G IQ 10 lQ l() tC) to \0 l 0 l () ID 4 10 I~ to 10 IC) l C) I~ I() I~ IC) 10 10 5 I() IO IC) I t) tD l t) \ t) \D LC) l C) ID ({) 6 IO 10 10 10 10 ( () (0 tQ IQ co lD 10 7
'C) l I:) l\) 'O I l) ID (Q ID l () 10 ID 'G A = Pan weight (mg)
Tr.
- color code:: ao.,,
Aunlysr: 'T5 "' IY.Co4 l"l ,4 \ /J*I. ~9 15.15 jLI, 30 15.'i1 15..39 J'i. 9J 15.15 15..t<o 14. '15 111.n Dnrc: t1.l"i -/\ I -18 U = Pnn + Lnrvnc weight (mg) Ann lyst: ~ '1.1.<<c -i., .<:\~ L,\.'2.-l U-'!>o 21.S"\ 1.~-~ "U .ti\ -z.1.'il 11.lO 2i.. fb '2..7. .'2.b 'Z.1.1_3 Ont,,: as. 1~-1&-
-C = Larvae weight (mg)"' U-A llnn!l calculn rc!l.
, .1..~\ ,.s~ i.,.,,; , . \ 'S ,.1..~ , . '=,\o t.-.;>Q ,.os , .oS l....'\O ,.2>\ lo.'\~
Analyst: j Weight per initial number or lnrvnc {mg)
- C / lniti:il number orlnrv:ic
,....~ w J::, :t, °" "',;;a '.!:><:)
"6' Q(., r>> ~ ~
o*""" llnnd cnlcul~tc!l. ,-...; 'O o*"' I 'fl \9
~ o*" o*"'
'J>
Annlysl: 0 o* 0 o* o* 0 o* C:>' Avcrngc weight ll(r Pcrceul reduction initi:il numbcr or rrom control (%) o. -, Y1 -s .c:; 1. c.,os - ~. & 7. o.,os -3.~7. larvae {mid J Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm - unusually small, lg= unusually large d&r = decanted and returned w = wounded. Comments: Page 29 of94 SOP ATIO - Exhibit AT20.3 rcvi ion 11- 1-14
' *- - - - ~ Page 4 of7 pccics: Pimeplwles pl'omelas Client: TVA/ Seguovah Nuclear Plant, Outfall 101. UV-treated Date: 05-15-18 Survival and Growth Data Day 100% lntake Control - Non-trea ted y z AA BB cc DD EE FF GG HH II JJ 0 (0 (0 (0 10 10 10 10 10 I
10 ( () 10 10 ,o lO IC) 10 2 {O ll) *o 10 l \) co ( t> 10 3
/ t) It) 10 10 \D co IC) ,o 4
10 Ia 't) I~ (0 I (:) (0 ( i:) 5 IO t 0 ID 10 l C) ID l(J 10 6 q ,4. ro IG lb ID 10 ID
/0 7
q I () ,o ID It) ( I:) IC) '('J..
,'\ = l':,n ,vcighl (mg)
Trny color code:: ~re..j Ann lysl: T.$ /5. 7'o !5.5G, J5.(i1 15.39 Jta5 /5.0a. J5.o5 15.fo9 D:11 ; ()6-0\-lg' B = P:1n + L:1r\'nC weight (mg)
/\n:1lyst:
~ CS-1..:?.*-*i 22.o\. -U.1.~ -u.,1.. ZZ . lb 2.1.'-\ \ "Z-2.../o\ 'Z\ ,'19 1..l :1\
D11tc: C = La rv:1c weight (mg) = B - A
~.1.8 S.loi c. .08 L,.11 ,.31.. -,,$'\ l.,.t.\-l 1..,.01-1-lnud cnlculntcd.
Analy 1: t}1 Weight per init ial number or lnr\'nc (mg)
= C / lnilial number orlnrvnc J, ~ ..J} ~ ~
,v' N' ~ 0 Hnn!I cnlculatc!I. ~"' '9 '-a ~ ~ '1) ....g o*"' o* . o*
l An:1lys1:
,\v mgc weigh! per t¥1.
Pcrcc111 rcd11ction o* D* lo'1..0 0 g.17. 0 0.1., %5 0 c) ivo, inilinl number or from control (%) Nfut.AW.
- lnrv:1c (me) 1 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk "" sick, sm = unusually small, lg "" unusually large, d&r = decanted and returned, w = wounded.
I Comme11ts: Page 30 of94 SOP ATIO - Exhibit AT20.3, revision 11-01-14
TVA I Sequoyah uclenr Pinnt, Outfall LOI Mn , 15-22, 2018 l'i111epl11tl1t.1*11r"111clfls hronic Whole
- mucnt Toxic.it Tc.1 l~l'/\-!!21- ll.-02-013, i\lcthotl IOO<J.0 Qunlit) Control Vrri lirnt io u or D11111 Ent ry, nl ul:11ion ', antl tati. ticn r ,\11 :11 xc~
Environmenta l Testing Solutions, Inc. rrojr( 1 m1111l11"1-; IJJ 7 Nat Im Con11tt1;auc~ ""*~<man. hnlo!ff'QJ ' jfiOc.MOJV 0C C**f'n1!1r.tl<"l t",\.1 K1'-pffnl, 1..i,t11 11.t.WUon .r il*8';,1t* i.llnM 1.n .... ,.,fu.k,o,,,:, n <<i,:btl~f"iH~ t\h:~11, ;.it.11 l."'1fid,M*f1..1'"'"11\ \\'(t'~l11/ h1ltl*lflllftll lf1" Mul!"4nkfll M t111t iot"l.:lt1 I r,~ C*~ !f1tl.ml f f " ( rti\ I f\1111-t\Nf ftNl1t1t*l'\ l ii( l.;f" ioe A*ll,f,ll*tli:;111
, *i.., ...... ,..""!h.. _ ,,.u 111.,m* , .. r l~n ...,. t-.:, **wiiJru I .:I
- U*A ,11,11,i..,"rt.. "' ", *I Swf"ll*t..;ft~ltff..f
~
'" ' - * ... . 11.,,.<1t%l 11t l...n.- (01eitt t"'4) bnut l ...
, .. ,bi.,
1'4* 11*,thtJ , ....., (:T 10 Ill 14 o, l l Ji 71!, 07.t. (I ?J(J Utl lt OI, Oil 10 IO i<Ol ll (.\ Bq II 75~ u1,,, 11 u n* u;,s O.M~ 10 9 '\ol .t 1*11Ur111 M< n u,,u II ~tJ
'*uH1 .:=- tfiJ ff 10 IU '"
9 1< Ul
"(IJ
? I 19
?I 71 6 JJ 1,02 Uf)f\'t ucm 111,ss
(-'on11ol , l l\t*1tr,1 1rtl C I IJ to
,n Ill 10 H>
ID ll *II ll ll ll 'l 22 ?* 18 ?~
- ?~ OS b gH b 14 bSl 11~~8
\IIIIJ OM\
IIL,11 ... 00 1*1 O(,< I 11100 IJ 6&0 S.l No1 .i 1111llt~hlf> u 10 IU I~ 13 2.1 76 7 6} U7ol 0 76J E 10 10 ll ?0 ll "1 7 JI 0 74 1 0141 I II) IU ] ol;l.1 7 ~IJ 0 7~(1 015') Ui11f\,. C 10 Ill
'" 7J IHI 111*1 7 2S U7?1 l! 7J1 I7 07?H 100.(1 il.~J J 1, ,
II 10 10 IJ l/,1 20 17 Hl U 7JJ 0 7,11 I Ill 10 ?I J7 728 0 711 0 7lR 11.,** J 10 10 14 ' " lbU1 IJJS 1]Jl HJ 7 ,., IJ 7JJ 0 710 0 , l t'J V 743 O 71n 10<].(J
"* ,. *IU "I. 10 10 IO II) 15 ll 116-l
.,.,M 7'0 oH,1 07<Q J U%
~I 0
I'
"'1n 10 10 10 II) 111 IQ 14 b-1 HJI IHq ll Jl 11 K3 ll ?J ll ?4
~2_10 7 lJ 7 51
~
71< 7> un.i 0 75'1 oc,H u ) lj
,1111
- 01J.I 075 1 oc,,,
0 715 I
*" . 17 ~
0 Ill IU 100 '.H sq 7!*1 IJ?:!S U7:!\J N!-~6"
- It
~ '"
10 ju Ill ll 87 l~ JQ 11 J1 216') 1.!6 t, 10 u T<t, ob:,,1 H"kl~ 17 0 ?lb U6ll' 1011.0 u.11 !> n . I 10 10 UY1 21 97 1Ul U7tll U7tJS II 1\1 10 D7l lHO . 7Q< 0705 0705 It> Ill IS 2<, ll lo Of,<JO 11, ,; - (>O() t)t,1 wo~. 10 Ill lJ ~~ 1210 71 1 07J1 U ,OJ ~~ ti 111 IO!l.tl * -J.li X IU w 141'> 21 7l b ., 06*lJ Ol.-!,l
\' 10 2211(\
IU\\'",.. l11t11kt
- ,. Ill '
10 IS7* 11 ~b !I !,,f 6.?11 HS O(t48 U5f~ U611 056$ 9B .7 AA llR In 10 J(l 10 l~t... IS JO ll 7l
,.., 16 I, UM 6 77
!lb(!., '" 0.08 0677 u.610 7.1 0671
~ ISO Minirnllm Slu1t1f1* HI 11{1,.*r,.:ft(,; Ou1111r11*, M U \ ;1 h1 t ~ PMSD P~m,M M*ni;,mm S111ufl am 1l1ir<<~**tct.' 1lMSI>: 9 f1\ tSO l5 n n*\\::t lr~O li.:t.1 j>fl.'\'.ll,K.'4"1 Tk l1~ISL) "' 'll\J1ltlll\ffTI I r<-,..,, ditr~ *d*1i,.:1W1.'l."U ti Clk\lf01 lnd Ir I\IJil llu1 '111 I~ ~l.u-..,hl llS1icallv I* nlit:mt Nl n \\ltOI cilh !fll' \ k:\C\ I I 1111:tkC'j Omrnt.u' M *u ,.ah* t : Otrl. 7 l..0\\-l:f r, 1*u bound d:lt,,'fll\il~ ll\ !I ' l:l<'.I\ t Htilt J ~U(litlt,,IJ ,~ t*MSI>: II . I V111wr 11 1SO uttd d.:h:rtn,!\CJ b) 1 £f';\ (O('th Jh."-'°out~l l 04'
*---r
TV A/ equoyah Nuclear Plant, Outfall I 01 May 15-22, 2018 tatistical Analy e
&w*rot1trtt11011 "fel-1lrw, SofatlOn 11\C..
Larval Fish Growth and Survlv~I To st-7 011y Growth S1art'Oa1e* 5/15/2016 Test 10. PpFRCR Sample ID* T\/A I SON Ouuall 101 E nd Dal e 5/22/2018 Lab ID ETS* nv,r. Tesung Sol Sample Type. OMR-Di charge Monitonng Report Sample Dale. May 2018 Protocol: FWCHR-EPA-821-R-02-013 Test Spec es: PP-Pimephales promclas Commenls UV-treated Cone.OJ. 1 2 3 4
\JV-Control 0 .6890 0.6140 0.9530 0 7630 Non-Control 0 .7360 0.7590 0.6440 0 6020 10.7 0.7410 0 7590 0.7280 0 7430 21 .4 0. 7280 0 7430 0.7190 0.7500 42.8 0 .7240 0. 7530 0.6750 D 7150 85 6 0.7290 0 7560 0 .6300 0 .7050 100 0 7050 0 6900 07310 0 .6940 lnla 0 .6280 0 ,5680 06080 0 .6770 Tra ns form; Untuns rormod 1-Tailod l&otonlc Cone,% M11an N-Moan Mo.an Min Max Cli'/ , N I.Sr.it Crltic~t MSO Moan N*Mo'"n uv.c nlro l 0 ,6795 0 916 0.6795 0.6140 0 7630 9 322 4 07191 I 0000 Non-Con trot 068.53 1 0000 06853 06020 0 7590 10.871 4 10.7 0 ,7428 1 083ll 07428 07280 07590 1 71 1 4 -2.351 2 4 10 0 0648 0 7191 1.0000 2 1.4 0 .7 50 1 ons 0735 0 0.71 0 0 7500 1 914 4 *2 063 2 410 0 0648 0 7191 1.0000 42 0 0 7168 1 0460 0 7 16 0.6750 0 7530 4 494 4 - 1,385 2, 410 0.0648 0 7168 0.9968 85.6 0 7050 1.0288 0 7050 06300 07560 7 663 4
- 0 .9 48 2 41 0 0.0648 0 7050 0.9804 100 0 7050 1 0288 0 7050 0.6900 07310 2 618 4 -0.948 2 4,0 0.0648 0. 7050 0.9804 In kc 0 .6203 0 905 06203 0. 56B<l 0 8770 7 306 4 Auxllla!l'. TttSts Statistic Critical Skew Kurt Shaptro-Wllk*s Te.sl l nd,calas normal dis11'lbu1ion (P > O 0, 1 0 .9.SS *1 Q 1Jij4 -0.0045 1 4844 !
Bartlett's Tesl lnd,cales equal variances (p = O 05) , , 2468 15 0863 The control means e no1 sign,ficantl)!'. di!feren1 <e ~ 0. 91 l 0.11761 2 44691 H;i:eothosls Tost (1-tall, 0 .051 NOEC J..OEC ChV TU MSDu MSOe MSB MSE F-f',ob di Dunnett's" I 100 >100 0 06483 0.09541 0 0021 0 00145 0 25399 5 , 18 neatmenls vs UV-Control l.ln*~r lr,tol'polat,on (200 Re samplo$) Point % so 95'Yo CL(Exe) Skow
,cos > 100 1c 10 > 100 IC15 > 100 0 IC20 > 100 09 IC25 >100 08 IC 4 0 > 100 07 IC50 > 100 06 C>
C 05 0
- a. 0.4 Iii 03 c::
02 0, 00
-0 I
-0 2 0 50 100 ,so Doso 'II, Ooso*Rosponso Plot 08 1-lall, 0.05 lcvel i 06 al significance e os 0
~ 04
,._ 03 02 01 o+--~--~--~--~--~--~---j
~
i Page 32 of94
TV / equo ah uclear Plant Outfall 101 - Intake Mc y 15-22 2018 tati ti al Analyses
,wltonm*nUI 1u1ln9 S0lu\J0n1. Inc.
L arval Fish Growth and Survival Test-7 Day Growth Start Date. 5/1 5/2018 Test ID: PpFRCR Sample ID TVA/ SQN lnta e End Date 5/22/2018 Lab ID. ETS-Envir Testing Sol Sampl Type. DMR-Discharge Monitoring Report Sample Date: May 2018 Protocol. FWCHR-EPA-821-R-02-013 T st Speci s . PP-Pimephales promelas Comments UV-treated Cone -% 1 2 3 4 UV-Control 0.6880 0.6140 0.6530 0.7630 Non-Control 0.7360 0.7590 0,6440 0.6020 10 7 0.74 10 0.7590 0.7280 0 7430 21 .4 0.7280 0.7430 0 7190 0.7500 42.8 0.7240 0.7530 0.6750 0.7150 85.6 0 7290 0.7560 0.6300 0.7050 100 0.7050 0.6900 0.7310 0.6940 Intake 0.6280 0 5680 0.6080 0.6770 Transform : Untransformed 1-Tailed Cone-% Mean N-Mea n Mean Min Ma x CV% N t-St t Cri tical MSD UV-Control 0.6795 0 9916 0.6795 0.6140 0.7630 9 3.22 4 Non-Control 0.6853 1 0000 0.6853 0.6020 0 7590 10.871 4 10.7 0.7428 1 0839 0.7428 0 7280 0.7590 1 711 4 21.4 0.7350 1.0726 0 .7350 0.7190 0.7500 1.914 4 42.8 0.7168 1 0460 0.7168 0 6750 0 7530 4 494 4 85.6 0.7050 1.0288 0 .7050 0.6300 0.7560 7 683 4 too 0.7050 1.0288 0 7050 0.6900 0.7310 2 618 4 Intake 0.6203 0 9051 0.6203 0.5680 0.6770 7 306 4 521 1 943 0.0757 Auxiliary Tests Statis tic Cr itical Skew Ku rt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95581 0 749 0 46261 *O 5692 F-Test indicates equal vana nces (p = O 60) I .9538?. 47 4683 The control means are not ignificantly different ( -= 0.91) 0. 11761 2.114691 Hypothesis Test (1-tail , 0.05} MSDu MSOp MSB MSE F-Prob df Homoscedast,c t Test indica tes no significant differences 0.07567 0.11136 0.00702 0 00303 0.17896 1. 6 Treatments vs UV-Control Dose-R spon e Plot 09 0.8 0 7 1-tail. 0.05 level l 0.6 of s19ntficance e o.s (!)
~ 04 a
,.._ 03 0.2 01 0
p ~
~ 8 i
Page 33 of94
Page 5 of7 pecics: .;....:.;-'-=Je..:..:..:=-=-=:....c..:c..::.:.;;.=.:.:= ate: os-r-18 Client: eguova h Nuclear Plant. ut nll 101, UV-treated Dally Che11,istr,*: Day (Analvst identified for each dav, oerformcd 1JH, D.O. and conductivilv measurements on ly.) 0 J 2 Anulyst rlJ' ,-,J t15 Hr ,0
/-1.1' Conccntrntion Parnmcter . 'tk,r., -
DH (S.U.) :,.9-:, "1.~5 .. -, :,. ~ 'Z :,_9 , '.\.tnC l co TROL DO (mg/L) Conductivity (11mhos/cm) 8.o 30~
?.."1 (-:f.'1) 8 . 0 soe
'":{.c_
- 311
-:/. fj ~-~
UV-treated
*All<alinity
~~ " -
(mgCaCO.;/L) \.gt) .,_ ~ -/* -
*llnrdn css (mgCaCO.i/L) g,,1.., 1
"~ ; !?'-\
" Temperature (°C ) "--.\-"'\ i,~ .'-\ 1.J..\., ""'L'\* '!, "l..'\.\o """' * \o pH (S.U.) "':(..9-=t. -:z_-::,13 -:/.98 -=,.-::re:; ::/...ttn I'.) ."1"6 DO (me/L) f:1.o ~ -"'1 R. o "':/-~ '::(.P,, 'l....t 10.7% Conductivity (umhos/cm ) 293 ?.t:ts 2~1 "Tcmpcrnturc (0 C) '\.."\., '"\....'-\,.'-\ "\..<\- i ~\~ i..'-\ ,1 ""'&.t.S nH(S.U.) ::f:-'18 7- -:f.(;, ? -98 ""+-=!- I +-9Af l\.~
DO Cme/L) f?.1 ~-0 R.o ':1.c, ~-a # j ~ 21.4% Conductivity (1u11hos/cm} 29,1./ 7.":/-(s, Zrf
- Tempcrnturc ("CJ "-""
- cf '\.A.'\ "'\.'-\ *.!- ""\..'-\*L- "\.'-\* 8 \.'\,i.
pH (S.U.} ":l-18 -=,. f-'A '1,9f{ 7- .::;2 :/.9J{ , ..J.u<<;' DO (me/L) e., '8.o R. t ~ - "::I ':{. .,, ~.2.
- 42. % Conducth-ily (u1nhos/cm) 2 4( 1 '2..4~ '2.38
*Temperature (°C)
DH (S. U.}
-i..'-\ . f
-=l,9S
"-\ -S
-=,.7.~
,_'\ ,K
-:,.9~
i..'\,--\
-:;.~,L/ '"'"" .,
~ - 9Z
"'-'\*'-'
'.+.-=.-?:i DO(ml!IL} ;?.3 fll.o f=?.1 "7. 7:/ 7. .,, lo.Pi 85.6% . Co ntlucl ivil
(µ mho~/cm) l":l~ /=fl 1'17
'"Temperature (°C) , -t .'\ '"L'-l* ,_ '1.'-1 *" "\..~*'-' i}\/\ \.~*'-\
' pH (S.U.) 1-9~ 1:=n . ~ -9G, "":/.(,, s- ~ . 9/ l:\ ."i(1 DO (mg/L} ~.g B*< R.-z.. ? . 1- Ftl.J lO,'"'.l-Conductivity (junhos/cm} /52 "'
IL/(p l"-!tp
" Alknlinity 100% (ml? CaC01/L) 5~ S'-\ -
"' fl:trduc s (me CaC01/L) ~Q 4 --
\a'-\
- TR chlorine (mg/L) ,o.,o -<-0.10
*Tcmpernture (oC) ""t.< . 0 L.4.*b 1..'{.'1 '\.'l- s 1.-\* '\ ~l-S.
J pH (S.U.) DO (me/L} 7...493 1?.~ 1-+I 9?. Z "7/-. 9~ Jf?. 2.
-:/.(;:,I.{
4 *1'
-:{. tJ-,
7.c,
'..J... ~.
IO.Oi Conduclivil}' - (µmbos/cm) /50 /'i:l 15-'f 100% -* - Intake
*Allmlinity (me CaCO;/L)
- 1111rdncss S\... - . l S\o
*,r -
(1112 CaCO;JL) l.,O loC ,, :,
"TR ch lorin e {mg/L) -<0.10 -<-0.10
*Temr,ernture (OC) 1.':i *O 1.'-!*'l '"LS, I ,.'i *S ~-0 1H-1 Initial Fi n11l Initial FinnJ lnilinl Finni 1pcralurcs performed at the time of test inu1a11on, renewal or terminnllon by lhe nnalysl 1dcnllfied in 1hc Dmly Renewal lnfonna11on table located on Page I .
..."Alkoh ily, 11ardncss nd total residual chlorine pcrli m1ed by the anal st identified on the bench sheet specific for each analysis and transcribed to th is bench sheet
,., .., L Y'.'
0
*
- Totnl residual chlorine was performed on non-trcn1ed Outfall IO I and Intake samples.
V,...., age 34 of 94 SOP AT20-Exhibit AT20.3 revision 11 14
l Pa e6of7 Specie : Pimeplwles promeln Client: TVA I Scouo 1ah Nuc lear Plant Outfall lO J, UV~treatcd Dale: 05-15-18 Day (Ana lysl 1de111ificd for each day, pcrfom1cd pH, 0 .0 . and conduclivily mcasuremenis only.) 3 4 5 6 nalyst l Conce.n- Param ter Ii'--* P.sc., i:a.i::.c.... I)., i,l..l J-fS /-f~ rl..f trntion oH (S.U.)
'..\-.lt!> ":}~':l- 1.oi+ 'l..cl ~ -4~ :i ~,:;- 8.o'-( -=I,. 8D DO (mg/L) RJO "f,O i.l ,,,_q ......., -:i. G., "':l-8 "':/.o Conductivity
(µm hos/crn) 3\2 . 31~ !n'--' -" 298
- CONTROL .
~-~
¥
*J\lknlinity l UV-treated Im~ CaCOJ/L) l.,~
*~
I""'..-'" .
~-Q,
- t1nrd11e s (m~ CaCOJ/L)
*Tcmncrnture("C) pH (S.U.)
i~
._'\ ,i e1'11
"'\..-\.':,
""l.514-
.~.os 8-1
--\., ""\..'\.::,
.ll..\l~
~-,
8ll'2.
"\..-\.* I.\
"':l. 'B"I
_0 1 ~ 1..-\*\ 8,.nc::-
"1..-\* ~
":{. "1B OO(mg/L) ~.o -::J.'! 'iJ .1 l\.,o 1..~ "?.'-f --:,. Fl "':7. l 10.7% Conductivity
(µmhos/cm) ~ ~'l.l- t.C\\ 2.it.
*Temperature (°C) ..-t-<f
""'""° i..'"'* &' "'\-lo -v-1 *"1 "'\.'f*~ "1.'(. &- '1..-l-S 21.4%
pH (S.U.) DO {ml'JL) Conductivity o0JJ\ 0.0 1,5cii
"::} <;7
<:/,OIJ)
'i. I
')-.,SO u,,q ao~
'4-,":l-i-;w
"?.es
'?. ,'-(
-oS 8-ci ~ ..
":[.y<j J.8.3
,,.v, 2t.-=t
~.
(umhos/cm) ~~
- Temperature ("C) ""\_'-\ ,! \.'\.lo ,..-\. 8 ""\..'-\*~ 1.'-\. \ ""L'l -lo '1..1\.\,
pH (S.U.) 911"\ I '1.~, ~.otp ':\-.SU, e.~3 "':/-.f!l'-1 8. <!:JG, ':/.R? DO (ml!/Ll ~.&' <i.1 l,f-1 ~.,- ?-!! 8.o -=t. 2 42.8% Conducti\*it * (µmhos/cm} i'?>1- ~64v a~v 240 qempernture ("C) .,.'l,'1 ~\.~ ~'\.( -'\.'t. '-\ '"'\..'-\,"'\ w"'\.l.\.*"L -u\.*" '"\.'\. s r>H(S.U.J 0.no -:}.--k., "i.0'3 *~.-ts 8 .03 ;..,~.:?c, 1 8, 0"'( "'=7-=T'-f
._Q£) (m,1./L) M\ "'1. lo 'ii',l *~.o ~-B ~ * '< 8-o 7.7.
- 85. % Conductivity (r1mhos/cm) 104 i<I+ l-6 ".} J-:/ I
*Temperature (*C) '\.'-\*"I "\..'-\..1.o 1..'\* C\ ""\.'\,'-I "\.1,VL 1.t.l,1 -'\.'\. "1-
""* e e.o~
pH (S.U.) DO (mg/1.)
"1P12 0.\ ,._.,
'":J..'+~ 'b.t>'+
'6 .,
ll.~?,
'.},.O 4.~
?.=,q 7,3 8-o~
8-a
- J_ ':/'-J
-:/.2 Conductivity (iunhos/cm) ,s0i l~o ' lll>2.. -*
/50
*Allmlinity 100% lo'"L (mg CaCO;/L)
*llnrd ncs I
-* i (mg CaC01/L) - *. \,.,,'-\
*TR chlorine (mg/L) <-o . 10
*Temperature (0 C) -u.o '1.L:, 1.c;. 0 1..l\
- S' ~ *t "\..-t*'i '"\S. 0 1.~ .1-pH (S.U.) '.1,.0,.C:, 1','S't "i".*'t ~-'1+ Bot -:,.. ~tt f:f.rt:>:J ":/.1~
DO Cml1/L) Conduclivit
- 0 ,7.. -:}-. " r.1 *~11 i'.l,.e) t-8 e., ~-1
(µmhos/cm) 191 1si (lQ\ .. J:;3 I* 100% Jnt.'lke
- Allmlinit ,
(m!! CaC01/Ll s~
*llanlncs (mg CoCO;/L) t.:.o
*TR chlorine (mg/L) ,0.10
*Temperature ("C) ,S.Q "l.~.lo "t.-\,'\ '"\.4. ~ -'\.C,. . 1) ~l.\*S 1S *O 1.~ - l..
Initial Final Initial Finni Initial Final lnitfal Final
';;,';:J;c, cratures pcrfonned at the tune oftest 111111011011, renewal or tcrm,nouon by the ~nal t 1dcnufied 111 the Dmly Renewal Information 10.blc located on Pa I .
...,.,L .a.Jl;;ill 1itY. hardness and total residual chlorine pcrfom1ed b}* the analyst identified on the bench sheet specific for each analysis and tra11scribcd 10 1his bench sheet
~ : .J"g' 'sj~ ref chlorine was pcrfonncd on non-treated Outfall IO I and Intake snniplcs.
O SOP ATIO - Exhibit AT20.3, revision 11 14
..._........_~-. Page 7 of?
l pecie : Pi mephales pro melas Client: TV A/ Sequoya h Nuclear Plant Ou tfall 1OJ, UV-treated Date: 05-15-18 Dnil)I C!le111isfr)1: Day (Analvst identified for each dav, pcrfonned oH, 0 .0 . and conductivity measurcmenls only.) 0 1 2 n:ilyst 6', LAS Mf AJJ ,-/(' ~ Conce ntration Para meter nH (S.U.) , . i c.. --:,.e3 , f* ?/9 ":f.:r:s 71. 6'~ ~ .<.Pl Control DO (mg/L) ' .<:\ 8 -o -:/--:/ +-C:, +.8 "'. 'Ji on-treated Conducth,ity (11mhos/c111) 3 \t.- 30$" 302. *: -
",\ 11.alinily to\ \..,C)
(mg CnCO; /L)
~ -"'"'
- 11~rd11c (mg CnCOJ/L)
*Temricraturc (OC) g 1-1..'-1..g '1.'l , ~
--~
, .A.\. "\.-.\ .--\
!l '-\
~'-\ -b
"\..'"'*'° In itial II Fin:il Initial Final lniti:il II Fin:11 Da (Analyst idcnlificd for each day, pcrfonned pH , D.O. 1111d conductivity measurements onlv.)
3 4 j 6 Analyst ,I'- &;L, &;v t..l ~ 1-1~ f/f/' J-1~ Conccn- f'nr<1metcr trntio n MJ'b5-iut6 pH (S.U.) 'l-.~Oi -:J..5 1 S.o'+ /+.'4~ ' - -,,, 1,. f: I ~.:, 'i "7.f!4 ontrol DO (mwL) l).q :J,,(p "[s ,O "4-..0 0J.)3) ~-~ '? .-z. i :':1 ":I. I on-treated Conduelivil * - 31'5 (11 mhos/cm) 3o"a joo 1ot;"
*Allrnlinily (m~ C11CO;/L) ~ ~ ,\ (.p \ '\_'IY,\ ......
*llnrdn s (mg CaC03'L) '"'. g'-\ *~
"~
*Tcm 1>crnturc (°C) ,A-'1 ,.~. s 1-'4 -1 i..'-\-~ "l.'-\. lo "\."\-s i..'-\.. ~ i.{, lo lnitinl Finni I lnitinl Finni fnitinl Finni I lniti:11 Finni
*Temper111urcs perfornied 111 the time oftest init iation, renewal or termination by the analyst identified in the Daily Renewal lnfornmtion table located on Page I.
Alkalin ity, lrnrdncss ond total residual chlorine performed by lhe analyst identified 011 thc bench sheet specific for coch analysis and transcribed to this bench sheet by: - - . - - -* Page 36 of94 SOP ATIO - Exhibit AT20.3, revision 11 14
TVA/ equo a h Nuclear Plant, Outfall 101, V-treated Ma 15-22 2018 Pimepltnle. prome/11 Chronic W hole Efnucnl To icit T t R-02-0 13, Method 1000.0 Dail hcmical A nnly e Enyt,onmcflt31 Tostlllg SoluUon,, Inc. l'rojccl nnn,bcr: _ _ _ _ _ _1_3_J3_ 7 Con<<ntrntion Pn 111 tr r Un,* U 0~1 I n.,,1 OoyJ lln1* ~ Dn,* 5 llnv 6 Initial Finni lniti;il n11 ul lnllial l'inal luilinl r,in:il lnllinl Fi1111I ln itwl Finni lnit ls l Flnnl 1111 (Slll 7 86 7.8 1 7 89 7 73 7.85 7 ss 7 '19 7 5I 8.!J.l 749 803 7 81 7.99 7.84 DO (m~/1, ) 7.9 so 77 76 78 6.3 79 76 80 70 76 72 77 7.1
~outrol. Co11duc1 ,vit1 l11mhw,/c111) 3 12 305 302 3 15 J08 100 JOl No11 .. trcntc1I /\llrnlinil)* (111~/L CnCOJ) 61 60 61 c:~co,1 ll nnlnl'>s (mW) .
I tm ncrnturc ("C) 1111 (Slll 2H 82 797 JJ J 18:5 2H 7'15 78:? M4 24 <> 7 'II Nll 765
.!4 7 19~
14 5 7 67
!41
~l 8 t\.l
_4 4 n4
- !H 7')(/
24 S 785 2-18 8 O.J 1~ () 780 DO (rnt!/ 1.1 8 (l 79 8.0 76 1R 74 8.0 80 8I (i 9 1b 76 78 7.0 ("ont rol, Contluclh*itv (umhos/cml 309 308 31 1 312 J l8 10.J 196 11v.1 , ~a tcd li\ lknli ni1 y (n,1(/L C:nC'O, ) 611 59 63 llur,luc~~ (111j!/I, C11 CO,) 81 84 84 Tc11wcn1turc (C) 141 24.4 lJ 7 :n 24,6 24 I, 2H 14 5 l-l? 24 3 2n 14 4 2n 243 11111 (SUl 797 77~ 7 9X 77j 7.93 758 8 01 7 ;;4 II.OS 7 -19 801 7 lW 8 05 7 78 DO /m~/LI 80 79 80 7.6 7.ft 74 8 () 79 81 70 77 7 ,j 78 7I 111.7'\i, Conducll\if Y(u111ho,/c111l 19 !'15 29 1 300 292 29 1 286
' r cm11~1~1turc (C:> .4 7 24 ~ 14 S 214 24 7 24 5 lH JH 2H ~46 1n 1-18 24 .!
lnll lSUI 7118 7 76 7\18 771 H -1 159 801 759 8.116 7 50 S 03 -7.85
- 3 8 OS 7 7()
2 1.4% DO(tn!!ILI 8. 1 80 30 7.6 78 7J so 7.S l! I 69 n 74 80 7I Conducln ii\ (11111 lm, lc111) 174 176 277 183 !78 274 167
'l'cn111crntu~ ,*c, 148 24 4 24 \ 14 2 14 S 2n '2H lH -H ~ .j 3 24 7 24 6 24.9 14 6 42,H'Y*
lnll lSU ) DOtnwll, l 7 9R 8I 7 7ll
- 80 79ll 3I 171 77 7 94 79 765 801 S1 7 61 7.8 S\16 81 756 69 s llJ
,., 7.84 7.3 8 06 80 781 72 Conducthit1 (umltos/cml 241 244 2J8 237 2-16 146 240 Tc11111eiit111rc ("Cl _4 ~ 24 5 148 14 ~ 2H N4 14 y ~4 3 -H !4 .I l -1 7 _4.J N9 HS 11,B (S Ul " 95 7 73 796 76-t 7 91 7 53 801) 746 805 14S 8 03 1.16 8 04 774 l>O trn!!IL\ s1 8.0 8I 77 79 bB SI 1.b SI 70 7.8 7.3 80 72 8S.6'Y* IConductil in* turnho,Jcm) 174 177 18.J 171 IS~ 187 171 Tcm11cii11Urc (C) 24 I} 14 2 249 1~ .J 249 24 J 24 Q 146 .4 9 24 J )48 2H 24 9 .n 11111 IS lf ) 7 9-1 772 79~ 7 65 7.9 1 7.50 H:? 7 46 Kt1.J 7.J3 SOJ 774 8.0.J 77J DO(mu/LI 83 8 I 82 71 8.0 t, 7 SI 76 SI 70 77 73 8,0 7.1
" umlurth it1 (11111ho,/cml ISl 116 146 159 1(111 162 150 10(1% All,alinity (11111/L C11 CO, ) 5~ 5-l t,1 llnrdn~, (tni:/L CaCO ,) (;(I 64 f!,1
- ro1:1I He,idu:,I C hlo, Inc (m~/L 010 0 10 0. 10
*r1:m11l'r,11urc !"Cl 25 U 1H 149 :!.J 5 249 _.J,5 15 fl _.J) .:!5U 2-1 J 2H 24 4 1!0 NJ nil (Sl' I 1 '13 7 71 79.J 7.6'1 7 89 7.ll 7% 7 5.J 8 ().l H7 81).j 779 8.03 77.J 0011110/Ll 8.3 81 S.2 77 79 c,*9 8.2 7.6 8.1 7.0 H 7.J 8. 1 7I Coutluc1M11 (1u11h o.,/rrnl 150 141 15J 1511 ISS 16 1 1}3 IIMJ' .. lu rn hc 1\llm llnlt) (mwL CHL OJ) S6 51l 51, lli,nJnc'" (u1i:/l. CnCO, ) 60 611 I,()
*Tomi Ht.,itluul C hlori11c (ruu/L , o 10 010 ti 1U Tcm11cr:i1urc ("C l 25 0 !-1.J 1, 1 ~.J 5 :!SO .!4 7 15 0 14 t, _J 9 ~43 .!S V .4 .5 150 :N l Nc,p fot I
- rduul chl<mnc was rcrformcd on nn11-1rc.11 d Ou1falf 1\11 ..J ln11kc *.impl
- File s n10 _051 51Schem Entered by T Sleeper Reviewed by -tJ:{--
Pagel of7 Ch roni Whole , ffluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriotlaplm ia dubia lient: Tenn essee Va ll ey utbority Co unty: Hamilton Facility: eguovah N uclear Plaut Outfall: 101 NPDE #: TN0026450 ProJect #: Dilulio11 prepal'atiou i11(or111ntio11: Comments: Diluuon prep (%) 10.7 21.4 42.8 85.6 100 Effiucn1 volume (ml) 267.5 535 1070 2140 2500 Diluent volume (mL) 2232.5 1965 1430 360 0 Totnl volume (mL) 2500 2500 2500 2500 2"00 Test or}!nnism source ill(ormation: Test i11formntio11: Or!!anism a!?e: < 24-hours old Randomizing template color: i\u.JP Date nnd times organisms were born o<; .,c;-\f C>l.,QO , i . IO'-\ lo between: Incubator number nd shelf location; 1-~l Culture board: US-o&*\k A_...........-0S-oi*18. & Replicate nur,ili~r- i I ! i . ~ ~ I s I 6 I 7 I s I 9 I 10 YWTbatch : c,.\ -1\,- l i° Culture hoard cup number: ~':> I~ I J1 1 J- I \"'LI I:'> I I'"\ I ~ l..x> 13 \ Transfer vessel i11fomm1ion: I oH: 1,'\~ S.U Temperature = "\..I.\ .~ QC Avcrnec 1mnsfcr volume (mL): I < 0.25 ml Sele11ns11*1,m batch; o+3o-,, Daily renewal i11for111ntio11: Day Date Test inith1tion and feeding, MHSW S:1mple numb er used Analyst renewa l and feeding, or batch u ct.I utfall 101 Intake termination time 0 05-15-18 llO~ O':.*lO*\f A, l f( OS l'*l.Ui... \ q OSt'-1 , lf"i Y\ l 05-16-18 ll"\t'\~ ~ *lO"'\~ (\ l( OS ti\. 01.. \~cc:-'* ~ ;1 2 05-17-18 "'
\Ol~ O~*\lc.--\if\ 180Sl'i> . o'-\ \.i-1.)S I~ , 0$ Jf 3 05-18-18 ll.) :r'\ t),S,\lo*t.k " \iO~tlc,. o'-\ ,eosib. o-; -*u 4 ~, V' 05-19-18 \t) "'l..\ os.* 1b*\ a~ 1i os ,~ . l>".:> \~0~\~ -~,1 5 05-20-18 ,cs-i., O~*l\.*18~ 1, !\C I 5( , O"". \ROS\J. o'-\ \,l 6 ,&-~ \!, o-; 1A 05 18 10 01.o D~*\\:i* liB \ &~S\i., CP.:>
7 0- 18 \.t:i'\..S Co11trol ill/ormntion: St1111111mJ1of test endpoints: Control-I Contro l-2 Accc pt:incc crilcrin % of Male Adults: 07. 0.1 ~20% 7-day LC;o > ID01 . % Adults having _,,d Broods: lnD 1- lOC> 7. <!: 60% surviving adullS NOEC \DCI 1. % Mortality: 01. 01 . S20% LOEC >1007 . Mean Offspring/Female: -U.4 1..'i.. 0 <!: 15.0 offspring/female ChV ') LOO 7 %CY : \o . ~..,. s.~1. <42.0 % IC2s '> 1001. Page 38 of94 SOP ATl I - Exhibit ATI 1.2, revision I 1-01-14
Page 2 of 7 Species; Ceriodap/111io ,tubia l Client; TVA/ Sequoyah Nuclear Plant, Outfall LOI CONTROL-I Survival n11d Reproduction Dntn Date: 05-15-1 Replicate number Dav .I 2 3 4 s 6 7 8 9 10 I You ng produced 0 /) {) /"', (") 0 () () () n Adult mortality L '--
'-- '- '-- '-- '- '-- '- L...
2 Young produced r, ('""\ /) (') r, n 0 0 0 0 Adult mor tality L L. '- \_... \._ L... '-- '-- \._ ~ J Young produced (\ n 0 0 0 0 D *O 0 () Adu lt nwrl:ilily L L... '-- '-- '-- '- L- '- '- l.._ 4 Young produced :-.. '-\ '-,\ ~ --\ ~ 5 ~ ~ -~ Adult mortalrt}'
"-- \._
'-- \.__ L '- '-- '- '-- "--
5 Young produced ~ \\ IC) I\ '~ \0 lO \0 \ L- \ 'b Adult mortality
'- '- '- '- '- '- '- '-- \.. '--
6 Youn g produced () 0 () b 0 D 0 0 n 0
\._
Adult mortality
'- \._
'-- \- \._ L \.._ L, l_
7 Young produced \ \o \ => \ => :\ \\. \ '-\ \ "L ,s l '-\ \I.\ Total young prod1tccd (,. <a "'2..~ "'2..."1 .,_ '\ :> ';> "'2..~ L..\ '"'l..fs '"2...'\ ""L\ Final Adull Mort:tlllY L '- '- '-- '-- '-- '-- '-- '-- ~ X for y d Broods -,c;._ )<. ")o(.. '-../ ~ )< '>(_ -x- ~ 'V NOi.: M<tll mortality (L
- r.,.._ D
- d~d).SEl
- spl' bcood rsi!l',ik btood ,p~l bct,<<<n 1wo d>ysl. CO
- c,,ry o**tr{cluprin~ corricd oscr wil l odult Jurin* lran,r<r)
Co11cefllnrtion :
% Mortality: I f"l1.
Mean Offspring/Female: I -U,-4 CONC: l0.7% Survival ruul.Reproductio11 Data Rcnli cntc 1111mbcr Day I 2 J 4 5 G 7 8 9 10 I Young 11 roduc~d o* 0 0 0 0 n {) 0 D () Adult mortality
"-- L \._ \._
'- L L '- L '-
'? Young produced 0 C) 0 C) 0 (j ("\ () () T'\
Adult mortnlity '- L '- L L L L L L 1....... J Young produced fl {) 0 0 0 0 C) 0 0 0 Adult mor1:11i1y \._ '- L '- '- l__ \__
'- L \_
4 \' oung produccll ~ 45 '-l '-\ s ~ I..\_ ~ ~ ~ 5 Adult morlality Young prodt1ccd Adult mortali iy
\<..
L
*~\._
L
\
\.._
l"'l...
\.....
\._
\Q I (:)
I-,_ L
\\
\.._
\()
L L Ii....
.~,~
6 Young produced /) 0 0 (') 0 0 () 0 0 D Adult mortality \_ \_ \._ L \_ L L \._ L \._ 7 \'oung produced \~ t '$ 11.. I'-\ l I... \\ \ ':) lR I'""\ To1al you11g produced Filial Adult Mortnlit,* L
"°!):,
- !> '1..
~1.
"l."'- "2."
l_.
~!)
"'2...'1 L...
~
\...._
\ 3'"L Nott: Adul r mon:,lit (L - 11,*c,. D
- dt'.:,d), SO :a- splh &rood (M.t,leh~ d split brtwcen l\\'O dn.y$), CO
- ctn\' over (offiprin~ e.trrie<t ovc( uit h ltduh dunnr,. 1ra.n.sfc::r).
Co11ce11tmtio11:
% Mortality: 07.
Mean Offspring/Female: 3 \.\
% Reduction from Control- I: -'\-~ l.
Page 39 of94 SOP ATI I - Exhibit AT! J.2, revision l l-01-1 4
Page 3 of7 l Species: Ceriotlnplmin tlubifl Client: TVA/ Sequoya h Nuclear Plant, utfall 101 Date: 05-15-18 ONC: 21.4 % Survival and Reproduction Data Rcnlicntc number Dnr l 2 3 4 s (i 7 8 9 JO I Young protlucctl ri 0 0 {) t'I 0 {") 0 0 0 Adult niortnlity L \...- '- '- L. '-- '- '- L '- 2 Young produced 0 ~ () n 0 ('\ (') t) () 0 Adult mortality
'- '- \.._
'- '-- '-- '- L '- L.
Young protlucctl ~ (') 0 0 0 D 0 0 n () L L. L- i..._
,\dull mortality L '- '- L. '- '-
-' Youug protlucctl c::. '-\ ~ ~ s '-\ '-\ '-\ ~ '--\
Atlult mortnlily '-
\.._
'- '- '- \... '- '- '-- \.._.
Young protluccd '\ 1.... \'"L \ I,'.) IQ I0 \-:':> '() \\ \~ \~ Atlult mortnlity L- '- \_
'- '- '- \..... '-- '- \._
G Young producctl (') 0 r, 0 r,, b 0 C) (\ 0 Adult mortnlity L.
,.., '- L. '- '- \.._ '- "\.._. \.._
7 Young produccll \ \a \\.c l <=:> \ \o \S \'\
~,
'\o \~ ! '-
To tal young protluccd =,'-\ ~'4
- >"1.. ~b =, \ ~ "1- -=>t> 3,t) 3\o Final /\dull i\lortali ty '- '-- '- '- '- '-- '- '- '- '-
~'Ole: Adull monolny (L
- Irv D
- dod). SD
- 1))111 brood (soosJ* brood 1plit bttw<<n two d*)'s). CO
- c:ir,y ovc.r (ollspnnc r, CJ.m ewer ,,,1h .iduIt dunnc 1uns er)
Co11centrntiofl:
% Morlali ty: 01.
Mean OITspring/Female: 31..Q
% Reduction from Contro l-I: -n.., 1.
CONC: 42.8% Survival and Reproduction Data Rcolicntc number D~y I 2 3 .j 5 6 7 8 9 10 J Young produced 0 n (\ () () () (\ () 0 0 Adult morllllity L. '- '- '-- '- '- '- \._ \. L-2 Young producetl ~ 0 0 (') () () 0 0 {) 0 A dult mortnllly \..- \..- L. \.._ '- \. \.._ \........ \_ 3 You11g protlucctl 0 0 D () () 0 0 t) () (') L L L Atlull mortality
'--- L- '- L '- '- \.__
4 Young protluccd ~ s Ll ~ '-\ s ~ '--\ '-\ \.\ Atlull m rlality
'- '- '- L. \.._ L '- L \.....
s Young produced \:) \ \ I~
,,_ 11- l L\. '.,_ *10 I\ l I!)
Atlult mortnlity \.... '- '-- '-- '-- '- \.._ L L L G Young protluccd 0 (', 0i 0 l) n 6 {) 0 6 Adult mortality L L '-- L \._ L L '- L \._ 7 Young protluccd I I., I'\ *S 11 ,g ,g q Ho I (s I\ Tomi yo ung protluccll
~"1.. ~s ~2- ~, ~'-\ ~\ 3'-\ ~ t:) !>~ ~\
Finni /\<l ull Mor*tnlit)' L. '- '-- ~ '- '- '-- \.... ~ '- N111~. AdulC n10n.uldy (L
- li\'t. D
- dtJ.d), SB
- spin brood {11ns;tc broods 11 bttwttn two d.1y.st CO
- arr, o,cr(otrsorin1! a.med ~tr ,,,.h,aduh dun.M uoosftrl.
011ce11trlllio11:
% Mortality: 01.
Mean Olfsprin.g/Female:
% Reduction from Conirol-1: -\i-o 7.
Page 40 of94 SOP ATI I - Exhibit ATI 1.2, revision I 1-01-14
Page o 7 Species: Ceriodllpl111ia ,tubia Client: TVA I Seg uovah Nuclear Plant, Outfnll 101 Date: 05-15-(8 CONC: 85.6o/o Survival mu/ Reproduction Dot" Rc111ic:11c number Dny I 2 J <I 5 G 7 8 9 10 l Young produced 0 n D 0 0 0 n () 0 n Adult m rtality L- '- '-- '- '-- '- '- '-- '-- '- 1 Young produced (") 0 0 0 0 () 0 0 (') n Adult mortnlity L '-- '-- '--- \..._ J \'oung produced (<') 0 0 0 D D a t\ 0 D Adult mortnlity
'- \._ \...._. L- '--- '- \__ L '-- l.._
4 Young produced '-\ \. lo "-\ 5 '-\ '...\ <;. \o 5 Adult rnorrnlity \...... '- \...... \__
'- \.... '- '- \.,.... L.
5 Young produced \ ""'2.. n .. \ "\_ \ :> 1'-\ '-'.:> \ L. I C) 10 . \~ Adult morcnlity
'-- '- '- '- L \...._. '-- '- '- '-
6 Young produced C) 0 D D 0 D 0 D a a
,\du ll mo1*1:1li1y \_ '- \_ \_
'- '- \.... \_ '-- \._
7 Young produced Total young produced L.0
- ,\:,
\ A-
"'::> lo
\\
~c;
,g
~s
""1.,.c:,
oC\
\ \.o
-~~ I
\S
~\
,<\
~'-\ ~s
\" \\
~
Fin~I Ad ult Mortality '--- '-- ~ '- '- '-= '-- '-- '- \_ Nott : Adolt mor11o1lity (L
- lfrc. O .:t. deJd), SO
- split b;ood (sinyl e brood spin bc l,,.~cn 'WO ti:i,J\ CO* c.:tr: over (01Ts:uri1111 c.arricd ovc, '"'" .1.dull du.rirut 4G1nsfcrl.
Co11ceflfrntfo11:
% Mortality: 01.
Mean O!Tspring/Pemalc: ~ '-\. '\
% Reduction from Control-I: - 2 1.-'\ 1.
CONC: 100% Survival mu/ Reprotluction Data Rc11llcn1e number On)' 1 2 J 4 5 G 7 8 9 10 I Young pr'oduccd n _o_ i) () n 0 0 0 0 0 Adult mortnlily L L L \_ L '- L.- L. L '- 2 Voung produced i) 1\ 7'\ (\ L) l"i (') c\ 0 0 Adult rnorrolity L L. '- L. L L '-- L L L J Young produced (') 0 () 0 C (') 0 0 0 0 Adull mort. lity
'- L.. '- L L L '- L L L
-i Voong produced 'S ~ s '-\ 5 5
Adult mortnli ly Young produced
\ -.\
\ ' l~
\o
\
'S L
\~
~
14 I.a i "'\ L 1-Z... L_
\ 'l_
\~
Adult morrnlity \..... '-- \.- '- '-- '- '- L '- '-- G Young produced ("\ () () () () () (\ 0 () C) 7 Adult mori:ility Young produced Totnl young produced fina l Adult i\lorlnllty No, . -
- ,i L
\...
\ ~ \i L
~:>
L. lt\ 31 L. L '-
"l.. \
~ '\
L Adut1 mon*ltty (L l"-c. D dc,d). SO
- split brood (s,ni;J. b,oocl sp~I b<t"roo 1wo d*yi), CO
- g L
\~
~ \.
L
\._
L. 0
~'\
L
~b L
ll L L
\~
"?I'-\
\
o,tr (ofT, n*~ c,mcd Ol'<t wuh aduU dunn~ tr.11uf<1 l* L
~\
b \.\ L.
~.
L
\q I.._
Co11ce11lrnlio11:
%Mortality:
Mean Offspring/Female:
% Reduction from Control- I:
Page 41 of94 SOP A T I I - E.'hibit AT! 1.2 revision 11-0 1-14
1 Page 5 of ? pcdc : Cer iotlap/111ia tlubin licnt: TVA/ Scguova h Nuclear P lant, Ou tfall IO] Date: 05-15-18 0 TROL-2 Survival mul .Reproductio11 Data Repl icate num ber Dav I 2 3 4 5 6 7 8 9 10 I Young produccll (") /") () 0 () 0 () n /') {)
;\ dull mor1a lily L \_ L- L- '- '- \.._.
'-- \...... \,_
2 Young produced l) 0 0 0 D c) (') (1 r,, n AClull mortnllty \_ '- \__
' - '-- '-- '-- L.. '--- L.
3 Young produced 0 0 0 0 D D 'C) 0 t) 0 Adult mortnli1y
'-- \..._ \.._. L. \_. L '- \..._
'- L 4 Young produced L\. '-l "!) "-\ ~ ~ ~ ~ ~ "-\
dill! morrnlilJ' \._ \_.
'-- \,_
'- \._ \_. '-- '- '-
- Yo ung produced \ Cl I ?_ '\ \ "L 10 I Cl ,o \ \ n_ I \
Ad ult mor lnlily '- '- '- '- '--
\.... '- '- \.... '-
G Young produced () 0 0 n 0 0 0 0 0 D 7 dult morrn li ty Young produced \ \.o L l~ L \._. l'-t I'-\
\_.
\ lo \\o L-
\~
I '-\
\.._
'~
r o11l i*oung prod uced
;:,Cl 1.."\ '2.. lo DO ~o "l'\ :) ""2.i oc "1.. 8 Finni Adult Morln lity '-- '- '- \._..
'- '-- '-- '-- '--- \_.
X for 3,d Broods
"'- --.,c. .....;:_ -)C ':,{_ ~ 7- -y._ 'L ':I-NUI,. Adult man lily (L
- Ii,,:, 0
- d J), SB
- spti, brood (s,nglc brood spl11 bcr~= 1wo da ,s), CO
- c:,ny ovct(o!Tuprin c;urird o*..rnilh adull d"""' tr.ansfn)
Co11ce11trntio11: CONC: 100°/c0 I n ta <.e S11/'V/.V(l I lllllI ReproriIucI"10 11 D na t Replicate numbtr 0:J) I 2 3 -I 5 G 7 8 9 10 l Young p rotluccd (j n 0 0 0 () () 0 0 0 A<l ult morlnlity L '-- \.._.
'- '- \._ L L- \_.
2 Young producctl 0 D 0 D {) () () 0 () 0 du lt mortnfity L '-- \,_ L '-- \,_
'- '- L \,._
J Young produced (') 0 D 0 0 0 0 0 (') 0 Adul t mortality \_. \_. \._ L \._ L L '- '- \._
~ Vo ung produced s '5 "-\ s ::> s lo '-\ 4 l...o Adult mortnlity L L L L \,_ L \._ L L- \...
- Young protluccd I "'L \:;, \ ,_ l \ 12) \1- 10 IC) 13 (C)
Adult mor1nli1y G )'oung protlucc(I 0 n (') {) n () () D 0 0 7 dull mor1:ili1y Young pr du cd \~
\S L
l ""\ \&
\~
~
\ "\
\
"" \
L
'\ ,, L l~
\...
To lnl young producctl finol /\tlult Morrn litY ft'IJk, Adull monafit (l
- lin*, D
- dad). SB *
~~
L-
~~
~
?)~
~q
~"-
~lo
\..._
~ "1..
~
~~
L-
- 1'\
\._
l,t brood 1u, It: brood $plit bctwcr::n 1\,-o d.J s). CO
- c:ur O\tflofTsorim:. c:1mcd D\ c.r , ..irh .aduk durina tl'l1.nsfcrl
~'S
\._
Co11cc11trntiu11:
% Mortality: 7.
Mean Off: pring/Female:
% Reduction from Control-2:
Page 42 of94 SOP ATI I - Exhibit ATI 1.2 revision 11-01-14
TVA/ Seq uoya h Nuclear Plant, Outfall IO I Mny 15-22, 20 18
~
CQ Veritlcntion of Ceriodaf)lt11ill Re1>rod11cfion Totals C1) ('I) [J111l,*nm<<nlf'J Tetl f!'lt Sntutln.*n, ll'IL. llt 11ic~Ut. n1111,t.u~ ri Ucnllta l\' num l.to.r 0 Doy fO IAI l)uy °fMAl
..... 1 0 I 2 0
3 {1 4 0 s 6 7 0 0 0 8 II 9 a 1(1 0 0 I I (I 2 0 J 0
~
0 0 5 6 0 1 0 8 0 0
~ JO tl Q f 1 l
0 0 0 0 u (l 0 0 0 0 0 0 0 0 0 0 0 0 Q JJ 0 0 l J 0 0 0 0 0 u 0 II 0 0 0 tl 0 0 0 0 n 0 0 0 0 0 4 J 4 J J 4 4 s l J J 1(1 4 ,I b 6 4 s 4 . s b 1 49 5 () II Jl) II 13 10 10 10 11 10 106 s ll 12 11 I) 14 13 ll 10 Ill 13 121 6 1 0 Ill u i'.I 0 0 15 u 0 14 0 12 0 15 0 0 C) b 0
- o 0
IH 0 11 ,~ 0 0 0 0 15 u 0 JQ 0 17 0 17? 14 JI 141 !O 11 lb 7 I') Toiol lS 2S 27 29 JJ 28 27 ?8 29 21 l8J I 0101 Jf, Jli J5 J5 39 JJ JI J4 JS JS ) 49 100% f< c tlirll f(' numbl'r ft t!- >fk ta lt nulllht"r 1);1' Tomi llo\ fo l ~f I 1 J 4 s 6 7 8 9 10 I ? J ~ 5 6 7 ~ ~ Ill I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 l 0 0 0 0 0 0 p 0 0 0 0 ! 0 0 0 Q 0 0 0 0 u 0 0 3 0 0 0 0 0 0 D 0 0 0 0 ) 0 () n 0 0 0 0 !) 0 0 0 4 s 4 12 s 10 4 IJ 12 l J 10 4 12
]
II 3 10 l 38 4 s 14 I s . 5 5 14 6 4 1.2 s 5 13 50 127 10 ll In 5 11 D ll IJ 13 I~ (, 1 a IS 0 18 0 IS 0 16 0 JJ 0 16 a i7 0 JS 0 IS 0 17 ,~,0 (, 7 0 IS 11
'S 0
1q 0
! I 0
18 0 20 0 17 0 IS 17 t) 0 19 0 ISS To to! 31 JJ }l Jl n 1') Jl 29 JI 3? J II -r.,., 37 Jl 37 JY 36 J? J6 34 34 J7 J6l Co ntro l-2 R1: 11ic:ak' 1l11.n1btr D,y R.: 1lic:1tc ftl1HJIJ U o~l' To lnl 'To1ol l ~ l ~ s 6 1 6 ~ l!I I ? 3 4 5 6 7 N ') Ill I 0 II \J 0 (l 0 0 0 0 0 0 I n 0 0 0 0 0 0 0 f) f) 0 l il 0 0 0 0 0 0 0 0 0 II l 0 0 0 0 0 0 0 0 !) 0 0 J 0 0 0 0 (l 0 0 0 0 0 0 ;I 11 II {I 0 0 0 0 0 (J j ~ J 5 4 5 ,n 0 s ,0 J J *I 4 4 4 ~ -I J 3 J J s 11 I! JO 10 10 IJ 10 11 11 I~ I 14 -~ 1u ll **
~
12 10 10 IO II 11 " ll 1()7 6 7 I) 17 0 lb a 16 0 18 0 16 0 IS 0 16 0 u 0 16 0 1'1 0 I<>* 6 7 0 lo ,. II 0 iJ ,. 0 u 17 0
]6 0
16 0 IJ 14 0 0 IJ 0 14(1 l'oUif J4 JJ JO JI JI 32 lP JO H JG J?O l'o(l11 JO 19 36 lll Jfi 19 31 27 JO 28 1'111 42 .8% Rcnlluh.* numt,e-r n c lllett le number lhj l '*t~I l>Al 1ulnl 1 (, I I I) 0 J 0
~
t) 5 0 0 0' 0
~ 9 0
10 0 0 I I i) l Q
)
0 4 0 5 0 0 G 7 ll 0 8 V 0 10 u p l 0 II 0 0 0 0 0 0 0 0 0 2 0 0 0 0 u 0 0 0 0 u 0 J 4 0 3 ll s 0 4 0 b 0 4 0 0 5 0 4 ,,u 0
~
1) 4*1 l J 0
)
0 0 4 0 5 0
)
0 5 u 6 (I 4 0 I 0 6 0 47 s ll II ll 11 12 I~ I? JO II 10 118 s 12 11 I! II 13 I? Ill JO 13 IU 116 0 0 0 0 0 6 l 16 ,o 0 u 0 Jq 0 18 IS 17 0 Jo 0 18 0 17 173 G 7 0 J[ 0 Ii n 0 I) JS 0 Ill 0 10 0 I(\ 0 P) 17 0 0 Jq (l 116 r... , )l J~ 3? 37 JJ J7 J~ JO JJ JI JJ5 1"tU \\ I J:; ll 33 JJ J4 36 J1 JJ JJ )~ JJ9 7
T A / equoyah Nuclear Plant Outfall 101 May 15-22 2018 h r onic V hole
- ff1ucn t Toxicity T es t (EPA-821-R-02-013 Meth d 1002.0)
Ceriodapl111ia dttbfo hronic Wh ole I fflucnt Toxici Tc
~
CQ EP R-02-013, Method 1002.0 CD t
....0 Quali ty Co ntrol Verification of Data Entry, alculation , a nd ta ti ticnl naly cs f Envi ronm e ntal Testing Soluti ons, Inc.
l'roj tct 1111111 btr; _ _ _ _ _ _ _ _ _ _ _ JJ_3_3_ 7 Concc ntr:otio n Re 111irn le nu 1nb c.r Sun:iv:,I 1\ crll!lC re prod uct io n Cormrit11t ot Prtcu11 r d11 1101, from (%) V. ) 1orf pri nit/ rcrn11lcl uartnlt u (Y*) potlltd con1ral t ) I 2 J ,I 5 <, 8 9 Ill ontrol - I 28 21! _7 2 3 1~ 27 28 29 27 ttiO lSA 6.3 No t npp licuhlc 10.7 % ' I )3 32 J2 19 21) n 29 31 31 ,oo J I. I S.I -9.5 2 1.4% 4 32 30 I 3I ]:! 0 0 3~ 6 100 n .o ,A -12.7 42 .8% 2 5 2 J7 3~ )7 34 30 33 JI 1(10 33.S 7.t - 111.0
- 85. y,, 36 <, 3S 5 ') J JI 34 33 3 100 3-l.\l 6.0 -22.\1 100% 37 33 37 <) 3b JC/ ]6 34 34 37 100 ,.:? 5.6 .z1.s 30 2 2 JO 30 2\) 31 ' 28 100 29.0 o\'o l 1lrtJ1 lic:1bl Co nt rol* 2 27 100% l ntul r 35 3) 3) ~ 4 J(, 32 33 34 35 1()11 33.!> 3.S -16.\1 0 11,ruu 111 1: M. D: M111111w111 1g111lic:u11 Dillcrcncc Dunn ctr's ,\ I , I) valur; :?.0~7 l'M 'D = Percent M1nm1um S1gn,licant Differ nee l' ~I 'l>: 7.2 l'MSO 1s o 11cas111c oftest rrcc1s11J11 l'hc l'M 1s the 111111,mum pcrccm dincrenc l1etwccn the conhol und tre uncnt Llml cnn I dcclnrcd musucall) s1*111ftcmu III a I hole cfllue1111ox1c1ty test ln tul c:
11 I ll llltNl 's M I) , 1 ~l11 c: 1.080 I owcr PM D bound dcu:n11i11cd by U l:l'A (10 ' pc1ccnltlc) = 13% l'M 0 : 3.7 Upper PM D b(,und dcterrr.mcd by U f:.JlA (90'" pcrccnl1lc) =47% l.o\\'cr nnd upper PMSD bounJs wcrcdctcnmnccl l'iom the IOU1and 90th pcrecnlllc. res cctivcl . 01 PM SI) d 10 finm EPA' WET lnterlnborat I) Vnnab11tt Study ( Sl;PA , }1)(11 , SEl'A. 200 1b), Shi'/\ 200 1a, 211 lb hnul Rcrmn l111crlnhont1nl)* V:1r111hi h1 y tuJy nt t,P/\ Sh11rt-tcn11 ' hro,11~ .ind /\cntc Whole lffOucnt T11 octl r .:. t Mdhod!., Vnh1111~-,; I <1111.1 2-1\ppcndts. El'A, g11.u-0 1-0tH .1nll EP/\*81 1-0-0 1-005 US E11v1ronmc11lnl Prou:c11011 /\,:ency, onomnnll, 11
TV / equo *ah uclcar Plant utfall 101 Ma J ~-22 2018 tatistical Ana lyse Conodaphnia Survival and Reproduction lHt-Roproduction S tart Date. 5/15/2018 Test ID CdFRCR Sample ID. TVA f SON Oulfall 101 End Data 5/22/2018 Lab 10 ETS-Env,r Tesuno Sol Sarni)! Type DMR-D19Ch ,ga Montto rig Repor, Sample D le. May 2018 Protocol FWCHR-EPA-821
- R*02-01 T t Spaoas CO-Cenodaphnta oubla Comments* Non-treated Cone-% 1 :z a 4 5 6 7 8 9 10 Control-I 26.000 28.000 27 000 29000 33.000 28.000 27 000 28 000 29000 27 000 Controt-2 30.000 29000 2 .000 30000 30.000 28 000 31 .000 27000 30 000 28000 107 31 .000 33000 320 0 32.000 29000 29 000 33 .000 29000 3 1 000 32 000 214 34.000 32000 30000 31 000 31 000 32,000 0 .000 30000 34000 36000
,2.8 32.000 35000 32000 37 000 34.000 37 000 34 000 30.000 33 000 31 000 856 36.000 36 000 35 000 5 000 39 000 33.000 31 .000 34.000 35000 35000 100 37.000 33.000 3; 000 39000 36000 39 000 36000 34 .000 34 000 37 000 lnta e 35.000 33000 33.000 34000 34 000 36 000 32.000 33000 4 000 35.000 Transform: Untranslormod 1-Talled l1 otonlc Cone-% Mean N-Mcan Moan Min Max C\1% N t.Stat Critical MSO Moan N, Moan Control-1 28 400 0 9793 28 400 27.000 33.000 6 55 10 32.683 1.0000 Comrol-2 29000 1 0000 29000 26.000 31.000 5 391 10 10 7 31 100 1.0724 31 00 29.000 33 000 5 129 10 -3017 2287 2.047 32 683 1 0000 21 ,4 32.000 1 1034 2 .000 30.000 36.000 6.421 10 -4 022 2.287 2 047 32683 1 0000 42.8 33.500 1 1552 33 500 30.000 37 000 7 071 10 s. 8 2 287 2 .047 32.683 1 0000 es e 34.900 1 2034 34 900 31.000 39.000 5.957 10 -7 262 2287 2 .047 32.683 1 0000
,oo 36.200 1 2483 6.200 33 000 39.000 5.646 10 , 8.715 2 287 2 .047 32683 1 0000 lnla e 33900 1 1690 33.900 32.00 36000 3 532 10 Auxdla!}'. Tuts Statistic Critical Skow Kurt K"lnlOgo vo est indicates normal d1stnbut,on (p > 0.01 0 .87338 1.035 IJ.37827 - 00943 Bartlett's Tes 1nd*cates equal variances (P ; 0.90) 1.590,49 15.0863 The control means are not s rornflcanllk'. differen t !e = 0 '3! o eo11a 2 1u092 H)'.~thosls Test (1-tail. 0,05) NOEC LOEC ChV TU MSOa MSDp MSB MSE F-Prob di Ounne1rs Te~t 100 >100 1 2 04668 0 07207 76 5367 4 00556 4 11 5, 54 T~eatments vs Controt -1 Llnoar lnlorpolaUon (200 Resamplos)
Point % so 95%CL Skow IC05 >1 00 IC 10 >1 00 IC15 100 10 ~ - - - - - - - - - - - ~ IC20 >100 011 IC25 100 01! IC40 > 100 07 IC50 > 100 oe 0 05 t 04 8_ 03
¥l 02 IX OI 0.0 ----0---0--0
-0 1
*O 2 0
04 -t-..--.~~-..-~~-.-...-~..--.~~ 0 50 100 o oso "I,, Oose-Rosp onso Plot
*S 40 35 C 30 0 1-ta,1 0 .05 level g 25 or s1gm1,canee
~ 20
~
IX 1S 10 5 0
~
. S? ;;;
cD
~
co
¥1 "
B Page45 of94
T I quo rah uclear lant Outfall 101 - lntal< May 15-22, 2018 tatistical Analys lnvt om,.ntal t01Un9 Solut fGni, fnc. Ceriodaphnia Survival and Reproduction Test-Rep roduction Start Oat 5/15/2018 Test JD* CdFRCR Sample tO: TVA/ SQN Intake End Date* 5/22/2018 Lab ID: ETS-Env1r Test ng Sol Sample Type* DMR-0,scharge Monitoring Report Semple Date May 2018 Protocol FWCHR-EPA-821
- R-02*013 Test Si;ec1e CO-Ceriodaphn1a dub,a Comments: Non-treated Cone-% 1 2 3 4 5 6 7 8 9 10 Control-1 28.000 28.000 27.000 29.000 33.000 28 000 27 000 28.000 29.000 27.000 Control-2 30.000 29.000 26.000 30.000 30.000 29.000 31 .000 27 .000 30.000 28.000 10.7 31 .000 33.000 32 .000 32.000 29.000 29.000 33.000 29 .000 31 .000 32.000 21 4 34.000 32.000 30.000 31 .000 31 .000 32 .000 30000 30.000 34 .000 36.000 428 32.000 35.000 32.000 37 .000 34 000 37 000 34.000 30 000 33.000 31 000 85.6 36.000 36.000 35,000 35 000 39.000 33 000 31 .000 34.000 35.000 35 000 100 37 000 33 000 37 000 39000 36000 39.000 36.000 34.000 34 .000 37 000 Intake 35 000 33 000 33.000 34 000 34 000 36.000 32.000 33 .000 34 000 35 000 Transform : Untransformed 1-Talled Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Cr tlcal MSD Control- 1 28.400 0 .9793 28 400 27 000 33.000 6.255 10 Control-2 29 000 1.0000 29.000 26.000 31 .000 ~.391 10 10.7 31 100 1 0724 31 . 100 29.000 33.000 5.129 10 21 .4 32.000 1.1034 32 000 30.000 36.000 6.421 10 42 8 33 500 1.1552 33.500 30.000 37.000 7.07 1 10 856 34.900 1.20.34 34.900 31 .000 39.000 5.957 10 100 36.200 1.2483 36.200 33.000 39.000 5.646 10 Intake 33900 1 1690 33.900 32.000 36.000 3.532 10 -7 869 1.734 1 080 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test 1nd1cates normal distnbution (p > 0 01) 0 94659 0.868 -0 4961 -02125 F-Test Indicates equal variances (p = 0 .44 ) 1 70543 6.54109 The control mean are not significantly different (p = 0.43) 0.80178 2.1 0092 Hypothesis Test (1-tail, 0 .05) MSDu MSDp MSB MSE F-Prob df Homoscedast1c t Test indicates no significant di erences 1.07983 0.03724 120.05 1.93889 3.1E-07 1, 18 Treatments vs Control-2 Dose-Respons Plot 45 40 35
., 30 c:
0 1-ta11, O 05 level of significance g 25 "C e 20 C. GI 0:: 15 10 5 0 (',/ ....
~ -e Q N
~ h u
Page 46 of94
Page 6 of7 l pccies: Cerio({ap/111i11 tlubir, Client: TVA / Sequovah Nuclear P la n t, O utfall 101 Date: 05- 15-18 D m*1V Cl1e1111stry: Da (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) 0 I 2 l Concentration Parameter Analyst H5 HS ,-t.J' t-1S ,._,,(' IA.) DH(S.U.l -:,,. p!(, f-* 9<,, "1-f3'7 :J.92 1 -~'5 -~Aq DO (me/L) f."f e.o ?-71 -=,. fj '":{_ '8 'lei Conductivit (µmhos/cm) 31-z. - 305 - :?oz . . CONTROL *Alknlinity '* - " :-;-,, (me CaCOJ/L) \p\ .....
~" '.,.,!'..'< I..., C) g-..\
' - - ,, .,.'I
*Hnrdncss (ma CaCOJ/L)
~1-
*Temperature (*C) '\."\ "\S. \ ""\..~.b ~-0 "'\.'-\.., ""\.S . \
DH (S.U.l ""::/-'9S -=, ..,-:, -=l,9(,, -:/.. '12 1-*Cf( 0.a'.) DO (mg/L) 8 -o -=,..q -:, ..::, 1-B -:,.!J 't.~ 10.7% Contluetivity (µinhos /cm) -Z.90 2£9'1 zq~
*Ten1perature (oC) "\....\ . 8 "'\...'\.. k -i..-\."'\ "'-'l. k -u,\. "'\ ""\.S. L oH (S.U.) '1-9ft> 7{.t:j{. "";/.9tJ f-*.,-z 7 ."J'Z l}_q ~
DO Cme/L) 8-1 --:,.. "t f?..o -=7, 6 =?-a q_t::) 21.4% Conductivity (11mhos/cm) 2:ZS- 1."1-8 ?.1-2
"'Temperature (°C) "\..U.- '\ ~-0 "\..'-\. -""'\ "\.s. o ""\ ~ *\ \S.. O oH (S.U.) -f- '!(,, '"::1.9(p "":1.96 -:[. q, -=,.9.:3 -'l.a i:;i DO (mg/L) f?.,, f's.o P..c:, +-8 -:,. 9 /ll'.l .
42.8% Contlueti *ity (um hos/cm) 241 "2.4~ '2.37 "Temperature (oC) pH (S.U.)
'\.'-\.'\
"7--9"1
\."'*
92 ( "'L~- \
"1-96 1.-\ -"\
7 . ?J,q t..4-i
":/.f!}q
"\.'-\,&
r.tM DO (mg/L) ~ -1 8 -a ~( 11. C/ Ef>.o (J.0 P:..G% Contluclivily (111nhos/c111) /'::/-2 /":l(o /+I
*'fen1oernture (OC) ..-;.o '\.-\ . & "'\.."'l-8 ~ \ .'\ .,_q. "1 -i'-'. ~
oH (S.U.) "'7-.9J.( -=l-9-z.. ~?5 '7.tUJ "':/-1'8 'l- t\"1 DO (mg/L) '8.-z Pl. I 8. I '(i./ £!.a A-A Conduclivily (111nhos/cm) / 5& . l"lt isa ;
" Allmlinity 100% (mg CaCOJ/l.) s~ .,
~h
*llnrdncss (mg CaCOJ/L) I.a& I.""C)
"TR chlorine (mg/L) <. 0 .,o <o .m "Temper:iture (°C) '"\.S.o "1.1.\., ., i>\-'\ "1.S. \ "\.'-\* t "1.' i - .f pH (S.U.l , .-,,z. 7-.92 '"':f,'fS' ~-8B -=,.q( ~q4 DO (nu!IL) ~-'Z. ~., fl. , 113-1 ff.o ~.Ot Contluctivil * '
tLf!1 1'19 /52 (1tmhos/cm) - -- J 100% lntnke
*Alkalinity (ma CaCOJ/L) 5<..:, ; *- ' S& 1,
- Hnrdncss (ma CaCOJ/L) l9G 1i,--\
"TR chlori ne (mg/L) <0.10 c(..O, lO
- Temperature (°C} "-"'--" '-'l* & .,_~ . i' t.'t - f -u\*\ '1.'-t . '\
lniti nl Finni Initial Final initial Final Te111peratures pcrfom1ed at the time oftest ini1ialio11. renewal or tcnnination by the analyst identified in the Daily Renewal lnfommtion lnblc located on Page I Alkalinity, hardness nnd 101al residual chlorin pcrfomicd by the onalys1 idcittilicd on the bench sheet specific for cnch analysis and transcribed to thi bench sheet by: -+i.---* Page 47of94 SOP ATJ I - Exhibit ATI 1.2, revision 11 14
I Page 7 of7 pecies: Ceriotlaplmia dubitt C lient: T VA J Scouova h Nuclca1* Plant, Ou tfall ] 01 Date: 05- 15-18 Day (Analyst identified for each day, pcrfonned pH, 0 .0 . und conductivity measurements only.) 3 4 5 6 A nalyst tz..<., AA~
~ rl.S rv ~ -
rlS Co ncen- Pll rnm eter tr:i tion , 1Jll (S.U.) ..lM 'i .ol ~.o-+ e,.o3 0 .0~ 8 -o'f -:,..er, 8-CH DO (m!!/L} Conductivity
~-°'
-3,.::;-
'i-1.o 3~
~.o , .J,S ~.'--
300
*'°1 ,+
305
'":/.-:/ 71,R
~*, "'-~.
(umhos/cm) CONTR L *;\l k:llillil)' (mg CnCO;/Ll
~.,L
' --~ \.p\ --- \..'I:'~ - - . '
- t b rd ncss (mg C:iCOJ/L) &~ v~
*Tcmper:iturc ("C) 'bt-1 "-S * \ "'-~- i "\S*\ ,.<\,& ._c;. '\... "'\.~ - \ t..'\. 4\
nH (S.U.) 0.00 1 .02- 'i.o3 e.111, A.a:+ 8..o~ 8 -o\ 8 ......c: DO (mlZ/1.) f.l-.O, ~.o 'i,o ':\-4 ~6 '"7i. fj -:z. ~ "::(. t3 10.7% Conductivity (r1mhos/cm) ~z.. ~g-o 2eo z~-
"Temnerntu re (0 C) \.4,f "\.'-\ . &- '-"\-'\ "'\.S . o "\."\.-<\ "l.S ,0 "l..l.\,! "\."* R pH (S.U.) 1J.. no ~.01, %.c<+ 0 .0'?) ~01" 8 -02. f?. o~ 8-o?.
DO (mg/L} '.\.Cl "R .&"l '1. £} ,~.'\ ~-~ "':/. .s_ ~e -=,.e 21.4% Co nduclivil}'
~62.. a1n ~7'0 (11mhos/cm}
"Tem pera t ure ("C) o.H (S.U.)
""\A*.t A,n l.c;..o
~.01,
"\.)\. '\
'B.ocf
~-0 0.o\
... "\.~
8 ,01'
"'\.S . O 8 .c,Z 2 7-Z
"-'-t. B 8 -cS
"'1-*\. f 80'3 DO (mg/L) 8.0 ~.o n,.ai>f ~ ii .Er t:\..C\ ~-+ -:i..a i-C/ -.:,. t?
42.8% Comluctivlt * - ~21-(11n1hos/cm) ~50 ~01" ZL/o
*T em perature (°C) 1!{. ~ 1..-\ - ~ 1.,.-{.'\ "'\S,.Q '1.1\ ,'\ \.~."\ l..L\., '\'i,. \
oH (S. U.) 0bo ,. .01 "+,Cfq (/J,/)0 env, ltf..oo B.03 !J.oz D0 (111!!/L) . ~ '";}.'\ -:J-.Oi ~.o , -4, "'\- i-B ':/.9 "':{.13 5.6% Co ntl ucth*ily {)q (11mhos/cm) l'lC\ l,'b Pt/
*T emper atu re (°C} i...'-\.,f l..'l , & 1..'\. '\ "'\S-l.. i.t..\ .'\ '"'\5.. \ ,._.... 4\ ,A. k 1>H (S.U.) ~.o I t',"ltp ~ t.>O '*\..°'v 0.0~ 8.,:,o 8 -03 ~ -98 DO (me/Ll {:;,0 *R 1',C\ '\{-i '-t- e, '"=1,g "':/-~ -:,.,,,.
Co nductivi ty t '54' lSS- tS\ /!)~ (µmhos/c 111) -
*Allmlinity JOO% c; ~
(11111 CaCO;/L)
~Hnrdncss
~~ le"
-* - . 1 (mgCaCOJ/L) -*
*T R chlori ne (mg/L) <..o . 10 >
1..q.4' J
*T em perll tu re ("C) uH(S.U.l \.""*"
~u l.~. \
'1,-,q~
i.S , 0
-::r *c:\ I.,
.,_"i *"
0.02.
-i..s.o 0-03
-UV\
6'-.::il 8.03. 8-~
,._':. *O DO (1112/L) t:S. 2. ":1 .q -:i q ~}~ Hf -;f.t, E:,o 8-o
~
Conduc tivity IW IS"t \'5'5" lsc, (11mbos/cm) *-** . *::- . -" .. - 100% "Alka lini ty Intake {mg CaCOJ/L} . '5 & - -
"Ji nn.In es ..
(mg CaCOJ/L)
~o * .
"TR chl orine (mg/L) <.o.1D .
*Te mperatu r e (°C) l.'"\.* J ""\.~ *f 1.s.o 'i.e.\.* ~ 1..'-t
- C\ ,s .o ~.o 1...-\.E In iti a l Finn i Initia l Fi na l In itial Finni Ini tial Final
*Temperatures performed nt the Lune oftest in1tia11011. renewal or tcm1111at1011 by the analyst 1dc1111ficd ,n the Dai ly Renewal lnrormation table located on Page I. Al kalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet s ccific for each ::malysis and transcribed lo this bench sheet by: --!1.--
Page 48 of 94 SOP ATI J - Exhibit ATI 1.2, revision 11-01-14
TVA / cquoyah Nuclear Plant Outfall IOI No n-treated May 15-22 2018 Ceriml"pl111in tlubio C hronic Whole Ef0 11en l To, icit Tc t EPA-821-R-02-013 Mclho<.11002.0 0:111. hcmi c:11 Anal 'C* E~vlronn e~lal Tesllng Solullon,, Inc. l'rujec1 nurnl er: _ _ _ _ __ u_J_J7_ Con(entrutl n l'nn,metcr O:"'O Uoy I Oo,* 2 l)n,, J 11:1\' ~ O~v S Un6 lnlllnl Finni lt1i1i:1I Finul lnili11I l' ioul lnlti:tl l'in,11 tnilinl Finni lui1fol Fiori! lnitfal Finni
'1111 (S lll 7 86 7% 7 &~ 7.92 7 8.5 7 '}<} 7 99 8 01 804 S 03 H 03 &04 7 9? 8 (1.J 00 (m~/1.) 79 so 77 7S 78 79 79 so 8.0 78 76 77 77 78 C.orulucfi\ ii )' (1.unho~/cm ) 312 305 .,02 ,,s JOS 300 lOS ontrol :AJk11linit) <rnJl/l. C:iCO,l 61 01 60 lln rdncs, (mµ/L (11CO,) 81 81 84 Tcu111erature (C) 2~ .7 l.i I 24 .~ 1$0 J.I 7 25 I 2,11 25. 1 N8 !5 1 148 !5 2 .n 14 .9 inll 1Slll 795 797 7 90 7 9! 7 91 800 8 00 & 02 S 01 8.03 !..07 8.04 ~ UI sos OO(iui:/LI 80 79 79 78 78 7 'l 7'J 80 l!U 7S 7,8 7S 78 78 10.7% 2'>0 180 180 Conduc11vi11* (11 mho,/cml ~8\) 19-1 192 ! &5 1 cm11crn1urc re, 1,1 8 1-18 241 21 g 1n :15 l 1-18 218 14 9 ~S.O 2~ Q J~.o !4 8 248 lull (SUI 7.% 7% 7 'lS 7 91 I *)1 1 ()<J 8 00 8 01 8 0.1 8 03 S 07 8.01 8.0 1 S 03 ll.-1':I.
DO (mi:/Ll ~ I 79 s l) n 7S 7.9 7.9 8.0 71) 79 77 18 7S 7.8 Comluc1hih* l11111 hu,/c1n) ~1* 278 in !82 26'i 170 H! T~m,..,r,lturc 1'C) l<l.9 1) 0 147 1S 0 N7 -50 -"*8 1S.0 24 9 . 25 0 2H l.50 118 14.8 IDll 1Slil 7.96 i 9(, 7 9S 7.9 1 7'H 7 Q<j ~00 80'! 8.o.l 801 SJl7 8 0:! SOS 803 IJO (me/L) 8I 80 80 711 7 'l 7 ') 80 RO 7.9 79 17 78 79 7S
-12 8"/.,
Conducth it,* (J,mllo,Jcm) ~4 1 1M .!..,1 250 :!J7 217 '!40 r~1111ker:1 1urc ('C) 24 9 ?4 8 14 1 1 1.9 lH 2-IS 1,18 248 1'1.9 lS 0 14 <J !~ Q 1-19 1S I 1011 (Sl l 7.94 1 *n 7 9(, 7 89 711,9 7 98 8.00 801 7 99 800 8 0(1 sou 8 03 S 02 l<S.6% D01m!!IU 8. 1 80 8I 79 80 s.o 79 79 7 I) li.O 77 78 7.9 7S IComlocti \it \' (J unhu,/cm) In 176 111 17? 178 179 177 T<!1tt i,cr:ilu1c ('<.') 13'(1 2U 248 1d () ?47 lH 148 24 8 249 lS '2 24 O 15 I 1*1.'I 24 S 11111 /SU ) 7 ?4 1n 7 9S 1 88 788 7.93 801 7 9~ ll 00 7 ')(, 110.J 8110 8 (ll 1 98 UO (me/L l 82 8I SI 8I so 7 ,, 80 7'> 79 79 i .8 78 1 'I 79 IC011tlucti~i1, C11111hus/cm) ISG 147 m 156 155 ISi 15.t lflh% Alk11li11i1y (no)!II. C:iCO,J 58 59 58 I hu ,Ines., l mi:/L C:oL01) lt8 t,(l b-1 1111~1 l{Miduul Chlorine (mi:/1.) 0 )0 ii 10 , 0 10
!'c1111krn tu re t"C! 25 (\ 24 7 14 IJ 25. 1 lH :!H 119 15. 1 .5.0 1~ 9 :!SO 24 9 :!49 .!S 0 111 11 (SU) 7.92 7.92 7 95 u~ 1 91 7.9 1 8.00 7 9S 7% 802 s.o 8 01 S 03 8 0~
OOt nn,/1 .1 82 8I 8I SI so 7.9 81 79 19 7.9 7.! H s.o 80 C11nd11c1h it1 lu n,h u,Jcml 11 7 1~9 ,~2 I ~~ 157 155 IS'1 100*,1. lnta~* ,\ll<ali11it) (1111:IL C:1CO,J 56 58 S8 11:onlncs, (noi:/L CuCO,) llO (M c,(l Tu111l llc,id11:il Chlu ri nl' lme/1,l 010 11 IU 0 10 l e 11111t n11urt 1'C) 2~.? 1,1 k :!H 11 S 1~ 1 2.1? JJ 8 14 8 1S 0 2H H'i ~~ II !S.\l 248
Page (oO Page _ _ , of_l__ Total Residual Chlorin e (4500-CI G-2011), Screening Whol e Effluent Toxicity Samples Analyst Date ana lyzed I t O= l 5 ,~e; Matrix: Water, RL = 0.10 mg/L DPD: I CHM \Cl.>"t Positive and Negative Control: Control Type Sample ID Result (..I) Positive Negat ive Negative co ntro l Positive con trol Deionized water Tap water " ' Sample screening: Sample Sample ID Sample characteristics Resu lt (,.I') number Posit ive Negative
\. .!{,~ 11..\ . CTL Tv{\ k n:,.~ \()\ no, ,-i\tr\. . r,,,,.,, '-.
, t oe... , '-\. -~ ~ \ ~f\l,(((_ I) c.<l-e +o.,, ru e,./ '
\. ~ O~ lS . 01- ct-)F\e_Lt:> no w\ C"\ cJ flf"t, ,/
I lc"()'S,\.~.0\ /' 1-\"-.Tf\t.)()()~ O~h" I (' l.OtJ. /
/
V
/
/
/
./'\o/
o)Y
/
,, V
/
/
/
/
/
.... /
Note : All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. A pos1tl\le resu lt indicates the presence of total residual chlorine above detection(> 0.10 mg/l}, which results 1n a pink to red color change with the addition of the DPD indicator. A negative r suit indicates the absence of total residual chlorine, which results in no color chan e with the addition of the DPD indicator. Reviewed by ~---.1!1,. ., -----1 Date reviewed ~-c,"'-'5. _.-'1-=
$--~'g"---~
Page 50 of94 SOP CB-Revision 4- Exhibit CB.l
__ _.._ Page~ Page _,.e.;:.;:...._
/ ~ of __J____
Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix : Water, RL= 0 .10 mg/L, Meter: Accumet Model AB250 pH/mV/lon M eter Analyst I T5 Iodide reagent : INR '6~ 9 Date analyzed O[ . . /5 _ /8' Acid reagent : INR g 35 Calibration: Slope (mV) 0.10 mg/L 1.00 mg/L (suggested ra!lge = 26 to 30 mV) Reference standard # INSS INSS /i /7 3 Note: For sa mples with a residual chlorine of > 1.0 mg/L, the samples mun be diluted to be within the calibration range. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/l) (mg/L) INSS /~ /7 0.50 0,53 10 Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO = {(S - D) /[(S+D)/21} x 100 (mg/L) (acceptable range = :t 10%) I~ c, * (J""t- -r..JP-.. SQ....> \C> \ 5 t. o.o 7 L Duplicate D <'.:Q.01 Sample measurements: Sample Sample ID Total residual chloriru? number (mg/L) TV < O.OS mg/ L Method Blank (MB) L: (),() 1..2 TV = 0.30 mg/L Method Detection limit spike sample (MDLs) 0.3~0
\ o<;.1'-l. o~ ,.J,... I ~r0 \~Ile L. O . OQ.~ ..,
_/
/
/
/
/
V
/
/
/
,<Y /
,,,,,./ ~
r----~
'\~
- (j>Y
/
~
~
~
Note: All samples were analyzed in excess or EPA recommended holdin tome (15 minu tes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/l) (acceptabl e range = 90 to 110%) INSS 7 0.50 0. 47Co o;o Reviewed by '----K~_ __. Date reviewed I C><;,\~*lr Page 51 of94 SOP CB-Revision 4- Exhibit C8.2
Page 62 Page _J_ of _J__ I Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples M at rix: W at er, RL = 0.10 mg/L I -,-~ DPD: I CHM IOOY Ana lyst Date aoaly,ed C- /fi"_ Positrve and Negative Control: Control Type Sample ID Result (.I) Positive Negative Negative cont rol Deionized water v Positive con t rol Ta p w ater V Sample screening: Sample Sa mple ID Sample characteristics Result (.I) number Positive Negative
\ f( 0~ II., 0~
*~*
\e _.,_ .,.. .
., -(..J,t,... \~~ \t) \
no CO /o ( 1 C.fc. Q("'
/ \i crs.1 as
- t. .
\, I 11'1 ,l\',(e__ no co lor I c/e,a r /
I ( OS \ '1 . 01- £N~,e..\...~ no co for , c lc.o.r ,/
\&OS.,-, .Q\ C..\.\ ~"P-.N~
/tfj'r,t- \ft//Ou) J e, k_ar /
/
/
/
/
/
/
,/
/
A/\y
~/;Y
- r
~
/
/
/
/ ~
Nore: All samples were analyzed in excess of EPA recommended holdins ume (15 m inu tes) unless o herw1se noted. A positive result indicates the presence of tota l residual chlprine above detection (> 0.10 mg/l}, which result s ma pink t o red color change with the addition of the DPD indicator. A negative result indicates the absen ce of total residual chlorine, w hich re sults in no color change with the addition of the DPD indicator. Revie wed by I /) 6-:-__~~-:_.-i Date reviewed ~===O~S~-~l"'\:_.-,... Page 52 of94 SOP CB-Revision 4- Exh ibit C8 .l
33
.._._ __ Page Page_/_ of _J_
Total Residual Chlorine {ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L, Meter : Accumet Model AB250 p H/mV/lon Meter Analyst I \.S Iodid e reagent: I INR ~2 9 Date analyzed 05-17-1 c;r Acid reagent: . IN R ~35 Calibration ; Slope(mV) 0.10 mg/L l .00 mg/L (suggested ran11e ~ 26 to 30 mV) Reference standard # INSS /{p/f INSS /(iJ 17 .~n Note: for sample$ with a residual chlorine of > 1.0 mg/ the samples mu t be diluted to be within the caf1brat1on range. Laboratory control sample: Reference standard True valu e (TV) Measured value (MV) % RS : MV / TV x 100 number (mg/L) (mg/L) (acce ptable range" 90 to 110%) INS$ /(i, t 7 0.50 0. 5?\L.\ /O ft, R 0 /o Duplicate sample precision: Sample number Sample ID Total residual chlorine %RPO :: {(S - D) /[(S+D)/2)} x 100 (mg/L) (acceptable range= +/- 10%)
\k O'S. ll,.. o-.\ ,v p._ \ C,.Q t,.) \C) \ s L 0 .0/}?
l Sample measurements:
' Duplicate D ~ 0.0/5 05-17-/'i 1.S Sample Samr,lolD Total residual chlorine number (mg/L)
TV < 0 .05 mg/l Method Blank (MB) .t. o.o,~ TV = 0 .30 mg/L Method Detection limit spike sample (MDLs) 0.313
\ k O~l'b .O~ \°'J " \ s(hJ I 1-JTA',(. ~ o.nn7
/
/
/
_/
./
_/
, /
\C{.,/
,, { \Y NJ/
/v')~
~
/
/'
~
~
Note: All gmples were analyzed in eJCcess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS : MV / TV x 100 number (mg/L) (mg/L) (acceptable rante" 90 to 110%) INS$ 1wn 0 .50 (\ SI~ 102..u /<) 0 Reviewed by !.____..A\_,,__ ___, Dat reviewed j O~
- n-t&
Page 53 of94 SOP CB-Revisi on 4 -Exhibit C8 .2
I Page (oL\ Page _L of _J_ I Total Residual Chlorine (4500-CI G-2011), Screening Whole Effluent Toxicity Samples Matrix: Water, RL = 0.10 mg/L I Ana lyst l \~ Date analy,ed 05- [ - / f!;' oPo : I cHM 100 4 I Positive and Negative Control: Control Type Sample ID Result{,/') Positive Negative I Negative control Positive control Deionized water Tap water v v I Sample screening: Sample Sample ID Sample characteristics Result (.I') num ber Positive Negative I \ It tt=...\!-~ "\" ~ '.Si:'i~ I()\ no co /or c. lc.'4 r fQr-11 c\ ~ ~ I I /
,~os. '< -o...\ ~ tl-l~-.:..e no c:o /or > d e-Q. c- *v I \ k or:. \ "- CS1.... Ft..}~,e..L~ heh.\- +~\ c./q.u d '-/ ./
\ (OC:.\<'\-0 \ ~ G\p.,""t"t ""-~006-A. /ts'1+ '{ti/OW\ C/ood'( v
., /
/
/
/ -
/
/
/
,\~/
~JY o/
,VI/
y V _/
/
Note: All samples were analyzed in excess of EPA recommended hold ing time (15 minutes) unless otherwise noted. A positive result indicates the presence of total residual chlorine above detection(> 0.10 mg/l), which results in a pink to red color change with the addition of the OPO indicator. A negative resul t indica tes the absence of total residual chlorine, which results in no color change with the addition of the DPD indicator
~ FA.L~e. P~\'t\l..le. f-o.t. -r, t/1\ ('S~~ SM'\.P,e.
A:,;5 \ b\.e ,es, ltJ ttJ!LFetl...AX.£. . Reviewed by l1---J1:1-l-t------1 (J'---- Date reviewed~ . __Cl~S-*_1~'\-
*I_J_ _
Page 54 of94 SOP CB-Revision 4- Exhibit C8 .l
Page 4 I *-- Page~l~of __j__ I Total Residual Chlorine (ORION-97-70-1977), Confirmation of Whole Effluent Toxicity Samples Matrix: Water, Rl = 0 .10 rng/L, Meter: Accurnet Model AB250 pH/mV/lon Meter I \"""::, I '6;Jj I Ana lyst Date analyzed Oo-1'1- L ~ Iodide reagent : Acid reagent : . INR INR
~35 Calibration:
I Reference standard It INSS /(; 0.10 mg/L
/7 INSS 1.00 mg/L
/G, I 7 Slope (mV)
(suggested range = 26 to 30 mV) d~ Note: For samples with a residual chlorine of > 1.0 mg/L, Lhe samples must be diluted to be w thin the ca librauon range. I Laboratory control sample: Reference standard True value (TV) Measured value (MV) % RS :: MV / TV x 100 number (mg/L) (mg/L) (acceptable range= 90 to 110%) I INSS /(, /7 Duplicate sample precision: 0.50 0,5/../J.. /(Jg J./ O/o Sample number Sample ID . Total residual chlorine YoR PD : {(S
- D) /[(S+D)/21} x 100 I \ s ()!,. \ C.* (f',. s (mg/L) 0.01.;z (acceptable range = :t 10%)
I 1-Sample measurements: 1-nJf\\
~Q.~ \C)\
Duplicate D L LO .0/Q -......... n.:-_1q-/~ Sample Sample ID Total residual chlorine number (mg/L) I TV< 0.05 mg/L TV = 0 .30 mg/L Method Blank (MB) Method Detection Limit spike sample (MDLs)
<O QJ~I
- o. 3:; l
'°T'J~\ S.Q.~ ,~e_ 004 I Uo<;.\i_.()'-\ t:. () .
./
/
I /
/
~
I _/
/"'
n ~ I ,...,.,.,,y
,(J-' ( D_/
{JJ/ I .,..
--\ ;::,,Y'
/
/
I /"'
/
/
~
Note: All samples were analyzed in excess of EPA recommended holding um (15 minutes) unless otherwise noted Laboratory control sample: Reference standard True value (TV) Measured valu e (MV) % RS = MV / TV x 100 number (mg/l) (mg/L) (acceptable range = 90 to 110%) INSS /Co17 0.50 c). 5J,..,:.,, /06.0 °lo I --~'-)'{. .,.__ . . . . Reviewed by .... Date reviewed I o S
- 1~-lc;f Page 55 of94 SOP CB-Revision 4-Exhi b it C8.2
Page -z..o Page l of~~- Analyst /1-...&sc.::.L-.._ _ _ _ ~J Alkalinity (SM 2320 B-2011) Date analyzed C>~*l"2. ,,'& J Matrix: Wat r, Rl = 5.0 mg CaCQ3/L, Sa mples are titrated to pH :: 4.5 s.u Titrant normality and multiplier determination : ntrant reftrenu Normality check Begin End Total Nofmarty (N) of HiSO* pH Factor or Multiplier j numb r 5 nd rd number ml ml ml (E)
= (S ml N zCOJ x O.OS)/ = O.ZS/E (acceptable range e 0.0180 - 0.0220)
* (N x 50000)/ 100 ml sample
>> N>tSOO INR "o!>ID INS$ lv~' o., 1:u, i~.s 0. l)~OO /0,t)
Laboratory control standard (LCS): l Re ference number ndard Tru value (TV) (mg CaCO,/L) Sample volume Be in m End ml Total ml Multlplier Alkafinity (MV) (mg CaC03/L)
%RS: MV/TVxlOO
( cceptablera nge = 90 lo 110%) (ml)
, _ _(.qq_:9
( ss \\,~t> 100 100 ,~.l,p J~~ ,. 'l 10,1) °I 'L c~lc. Duplicate sample precision: Sample Alka linity S mple Sample ID olume 0 t*n End Total Mui 'plier (m,: CaCO.J/lJ (ml) Ill ml so Ja. Duplicate (D) j :::,.0°1. os 61<
.V\.l.
ample mea urements* <<... dS*,ug Sample Begin End Total Multipli r Alk.lllnity Sample number Sample ID volume (mll ml ml ml (ma CaCO,/l) MB (TV< 2.S mg/l) Oeion zed water, pH = 5'.~\I $U 100 o.o 0.1 \O;O j,O 0.1 100 70, '1. ~Q.I,, )."'i I'+ 5"0 ~ " I,, 3Y..~ ~ .; lo-o -:19.. C\ Lf-.1 ;). 3.~ l \ .14.~ ~ .1-\S.$ 6,:!> I o.o t,.u l,. ,o OS, \O ,t-e A I 1o .o ,~.\ l,. J J );!,. \ ,,g,; t,:i.. 1.#2. l"a.1.. ~g.~ J1:1.2.. 100
,!l,5 3<t~ 11.~ I\O a*ts 4-"'t. '6' )(),0 100 D ,O 9.i ~.i O'S. Dti* J8 ~.% Zo .'t lo Ir I I t:>
iof+ 31.1, h.l-QA. f.\-l Dm (2... '.2.1. 1, 3'"T.9 t,.~ Oo A l. DM<Z.. o'l-.q # .~ ~.,
~A-c. Otnfl- O.b 10.l> \0,1>
A-eU- Dn,fl..- \0$ ~.::i .... , ,. 'l. \U"\ La {Y)(oA-<.- rnec.n...
- control standard (LCS}:
DfrV2... Dma, ,__
~~.C>
31..i 2..l.i "B,C:, 10.6 l\. \ - l\O Refere nce st.l nd:ird B gin End Total Multiplier Alka linity (MV) % RS : MV /TV 100 r !u....L~TV~li--..:;;Sa::.:.;m~p=le numb r (m g C CO.J/l) volume""" ml ml (mg caC0-3/l) (ao:eptahl r n e: 90 to UO%) (ml) INSS 100 100 R vrewed by: Date reviewed: ( 05,BIB Page 56 of94 SOP C - e 1s10n 4-Exhibi C6.l
Pag Page 2- of__.__ _ Analyst Date analy2ed l&4S'- US*\'l,*,?, Matriic: Water, R : Alkalinity {SM 2320 B-2011) s.o mg CaCQ3/L, sample!. are rated to pH= 4 5 S.U. Titrant normality and multiplier determination: 8 in End pH F ctor or Mui lph m m (IV l\ soooo)/ 100 ml mpl *
- Nx 500 INR INSS laboratory control tandard (LCS):
tnd R ference stand rd number T ue value (1v) (mg CaC01/l) 5 mple volume (mL) Begin ml ml Total ml Multipll r Alkalinity (MV) (mgC... o,/L) "
- MV/TVXlOO (accept.a I t"ilng = 90 to l lOW.)
INSS \\o3~ 100 100 o.c 9.,! C\.~ \o.o C,"t, 9S.t> 0 /p Duplicate sample precision: S mple Alkallnlly %R PO Sampe Sample ID volume Begin End Tot I Multipr.e, (mg CaC03/L) :. ((S - O) /l(S+D)/2)) lOO number (ml) ml ml ml (acceptable ranC"e : r 10%)
\ R 6~o'\,\& U"r6h1*~~ '\$ 14~ 10.0 ,. s
- Q,~ ~ I,. l~o
\
D t, Duplicate (DJ l- !-+.~ 16..'1 'i,S )O;<> L*I) )i-o tl,<;1., -b..5*12.*l\' Sample measurements: Sample 8egon End Total Multiplier Alkallnitv Snmple number Sample ID volume(ml) ml ml ml (mg CaCOJ/l) Mti, V' - _ ... .... -~- ,,orf W:ttPr nH : < *--- 10:0 - ~ ~<;;*\'2'\\
\ @. c:,510 . 0 ~ t h.n m ho. 01...a ;,,5 ,s.q "7 ,.._q ?_,O >'-) ~~~ ....,, ...... ~ , loSoi.1.0 "-r../A 5~ Prtn 5o ?£\ ,Q ~S.'S'" --+:~ .,.\ 91-. , ~oso2. *u AtniNt- .,3c;,S' 29.1., '°4,1 :i,\ ~ \le.= ,'12,\& Pih . 2."I.'-" O"'+,l 4-5 ')_) ~Q l
t&oc;a.f.\" Jc.OSo& . o\ rf\l A BF I\.\ flt\ ir-Jr I 5o M.,
?,~~
2.~ .4
'+I.~
~.,
I-,,, 1'1'.'\ g\p V2-
,e oso~. ')J I 1... \ 04.\ ~ ~I,.. :i.., . ~ lo '2- \.~O~\\.\" j .3 ..L ~.\p '-1-~.C\ 3.~ .'2., L,lp , tosoe.O"\.- rfJA- f>f N 1>JT I ~ 0.1> 8.1 31 ~, lo-Z. \ &'OS 0"\, 1.~ I z I ~-1 1# ,3 ~.2. .., \ L,\+
J.-.
\ E-<>~ \ \. l 4\ J 3 Ul,? '1.5 3 2. .')'\ lf't \ ~ n~ 4f1.IC> C°fl.J.-mar\ I ~."5 9.S 1.1. \,, :1 I I u.' '&+ , ~ o~o°'. i..-i.. I '2. I II ,It, 13,,'I ~.2, 4.L) ql..
J-
.1 3
,. roS \\. \t
\C: DScn.. o"'\ ~+-tn~r\ <<w t ..1-S
\-\ .q l\eS
'"'5 1!4
;l "' I i..)
I Gt u\ IO~
":l 1.>
\ k 0~ 0 °'* °1c'!> I :i. I \'ii4 ~o L\. ~ ... u'\ <;n
\< O<;\\. \ °'- l J ..J- .2.10-+ .,,) 'l .,. ~-3. 11.4) C\2.
~~hn&v\ Anh -:,1.,
\kOScn.,~
\ ~OSo'\ , l-"L- ~ '
2-RS _L
.:l~.:1-o\--4>~
.Q4,.lr:,
;lit .j l'l
~.;
14)
- 14) - - 'h Laboratory control standard {LCS}:
Refer nced.,.ndard True.valu (TV) Sample llecin End Total Mul plier I allni y (MVI % MV/TV JOO number (me---.. ~, *-***-e ml ml ml (mg CaCO,/LJ I ccepi.ab range 110") (ml) INSS 100 100 B'>t. S, 1'2.**8 Reviewed by ate r vi wed* L.:O :..'S::.:. :.17:::>:J.>G- - - ' Page57of94 SOP 6-Rev,sion 4-Exh bit C6.1
C Page 1-""2.
,," . Page 3 of_4-____
C: : - - _:;_-: _) , . . - . , . .. fnljloof; ~ .... Analyst I E,'51,.., Alkalinity (SM 2320 B-2011) oate analyzed OS',\"2.A'zS Matrix: Water, RL = 5.0 mg CaC03/L, Samples are titrated to pH= 4.5 S.U. Tftrant normality and multiplier determ ination-Titt~-M _Normalitv check Begin fnd Total Normality (N) of HzSO, pH Factor or Multiplier sta ndard number number ml ml - f~ ml NazCOJ X0.05)/E = O.ZS/E = (N x 50000)/ 100 ml sample (E) (accepta~" n,so. 0.0220) =N X 500 INR INSS -e:s.... 't)C5. \'2. ., i Laboratory control standard {LCS}: Reference standard True va lue (TV) Sample Begin End Total Multiplier Alkalinlty (MV) %RS= MV/ TV><l00 number (mg CaCOJ/L) volum ml ml ml (mg CaCOJ/l) (acceptable range = 90 to 110%) (ml) INSS 11.c2,"3 100 100 2.tp,1 2J~ .~ 9.'9 loo 'll.# q~.oO/p Duplicate sample precision: Samp le Alkalinlty %RPO Sample Sa mple ID volume Begin End Total Multiplier fmg CaC0;/1.) = {(S - 0) /((S+D)/ZJ} x 100 number Ao H (ml) ml ml ml (acceptable range= :t 10%) s
. \& <:Ast mu""' 6 ;;i_5 ~.s lo.a 100 J_ D
- l. Duplicate (D) 1.
Sr:mple measurements:
. , "'--*- 2ft*,o 1+1~ .:? .S
~ -
l.:>O ~
,.---i)°S,l'L**.'g Sample Beg:n tnd Total Multiplier Alkalinity Sample number Sample 10 volume (ml) ml ml ml (mg CaCOJ/L)
MR 1n, - ~. r ~- -,
*. __ :onirea ** - L - - .... U - . ' -- S<ir,. - O'S*1l*\
'-' o<. o-\. o'-\ Q:rs+<<-tC("' Anh (lw l 2.5 '+\ .'5 '+S.S ~.O I Ll\ t O:O
<jC
, ~ osoci. . 1.~ I 2. I ~&.S -4:5',b :J.1 ( ln 8'4-j 6 .!-
I &0S 1I. 1"-. '-4-6.ic '4-"1 :'l ~.~ I~ "bB. oS * <r:.
- lk' A ml-\sw fh'\}; I 0,0
,:,~ - t.fb
- i& 6 l 2,
- }"00 lq , \
lc, , I lo.I v,.O
- l.Dl Lq:.::,
-- i I ~-1
(\~* c.:,i. \ ~ J 3 .L \;\ . I I~.?- "- *I UZI I f 'O':, Oi, O\ ~-PiCV ' Joe>
- 18 . '1.. ?~-.'1- "+ s J.!15 I&. oSO<\* 1.\ I 2- Z..1.:1* .?~.'e, '+.I "f\ \KOS.\\, \S .1 2 ~1.11:g 01.-i. ~* 4-4 , .e-oSl\k .ol.. l\oWnt;- ha<<"
I f OS 10 . crz_ I '
?-
Q,
~ 'I
~LL 5-c ~) .2,..
&~.1 Y-,,'2.-
~re~ ~.'&
2..o I."?.)
- 1..)
?_,
~o 91o
\ ~eiSl1. . O\ l 3 3if,o 4.<.? 4.'2? ci....
, kri:;of. o"1 i?)rc.lJ.P t &; lf~.i 4<<-o 5 .-i /'7..o) )O'C> t)
\ "0S10.G3 I :i I o.~ '+~ .i...1-~ l 1...o} q,,,o I K"0'::.11.. <J1... J .3 .L J.+.& °i.3 '{,S- ~) q.siO
, ,~ o~ .oR (_;,("\f'.. o..l htnd I 2. 9-~ IS.$" t., :i f
.....,, ~I ()'C
\kr,<.10. !)~ .JI 2 I \S'.< ~ 1.<I l,U. I C'"o) ?,t,oe>
18.~r,-~ - J .3 \ ..i21. "I ,qll.'- C,,4- ( C"'O'I .;-z.oo
\ $.s:OS<>~ ,O'\ ~ ...!- ~g.; ~Vs
-1....J o!o*O PAsQ111 ~"'"'- 4,,o r t'"o )
Laboratory control standard (L CS}: True value (TV) Sample Begin End Total Muitlplier Alkalinity (MV) % RS=MV /TVx 100 (mg CaCOJ ml ml ml (mg CaCOJ/l) (acceptable range '° 90 to 110%) (m l) INSS 100 100 Reviewed by : Date reviewed : I05*~16 J Page 58 of94 SOP C6-RP.vi~inn 4-FYhihit (.fi.1
Page '23
, Page ~ - - of 4: .) ~ilJ.,; ,; ~; k Analyst Dat e analyze d Ii---o=S"'-.,-2.-,-,'B- -
BSL
---i Allcalinity (SM 2320 B-2011)
Matrix: Wa t er, RL = 5.0 mg CaC01/L, Samples are titrated to pH :: 4.5 S.U. Titrant normality and multiplier determination: Titr;,nt- ri,f,.rence Normality check Begin End Total No rma lity (N) of H:SO~ p H Factor o r Multiplier num ber stanaa ru ,. u, _, ml ml - (S m l Na: C01 x O.OS)/ E c: 0.2S/E = (N x 50000)/ 100 m l sample (CJ ~-.,cee-* - L -~--- =- 0.0180 - 0.0220) ; NxSOO INR INSS - Se,._ O:,*IL.. *1 5c . Laboratory control standard (LCS)
- Refe re nce sta ndard True val ue (TV) sample Begin End To tal Multii,lier Alka linit y (MV) % RS: MV / TVX 100 number (m g CaC0 1/L) volume ml ml ITll (mg CaC01/ l} (acceptable range = 90 to 110%)
(ml) INSS \\0'3(3 100 100 ai .-; 4\.'\ cu,, lo.o C!ID.O C\ ie .C ,:,/o Duplicate sample precision: Sa mple Alkalinity %RPO Sam ple Sample ID vo lume Begin End To!al Multiplier (me caCOJ/Ll {(S - D) /l (S+0)/2]} x 100 n umber (ml) ml ml ml (acce ptable ra nge = .t 10%) s C2,o:>O j
\~<61~ . GS ~o. s.c:, l..O t().rJA; 2. i.. o.o 3 ,9 3.4 I co) lo.O A'AA8 i->
0 "Z()Oc>> \. l Duplicat e (D ) L
'. . 3 .4 +. ,g 3.~ I yo) 10.0 C
P..Si- -t:.S('?., / Sample measurem ents: Sa mple Begin End Total Multiplier Alka li nlly Sample number Sam ple 10 volume (ml) ml ml ml {mg CaCOJ/l) M B (TV <..2." ~.'~, -. * : __ ... YYOL 'il; J , t- _ _ _s.u. J UU EK.,<., ~-,z.,f.
' ~ t,<; ,,.
- c:i4 .Pa~ w k,,1<.. 3 2- l.<l \"U) ~ ,_ ' 1rb) IC,o 2,1c t:>
l(OS.c,L\0 c . ~tlc...h ( Sl c; 1'2-C> 15,; 3.~ I 1.1.\ l.~o
\/( (610,[)lo ( 2. I \'5 ..; I'~.; .2. 0 .1-1) l'w>
..1-IX'()':,\'"l *OS \ ...3 16.~ 2,'0. cy ol.~ Iq) 10 0
\ Ji ~S6k , I\ P,* Mil (>.... I ;;5 ?,.o .'l ~4.'ii ~ .'I ! ~) .. tt:,o 2.
\ ~ (1:110
- Ui I <<4,j ~ -'+ ~"' l-4) I /.In -
{ l 8 oS 1"1. .o\o j ..2, ....L ..,78.4 ~ _2,. ~ I i4,) .H-'7.<:.~ 06-11..1-BJl. Sa ..usw - lo<' -~ .. i+'l .\ o lo .<;'" ,. ~G , ~*-(1oo) 05.\'LlRi o~ { J_ ~ ~ >. = .q 'l.~ --- - ~~ 05 1'M(j
~
J
/
/
V ( Bi;t.. ~-- .- *o - Laboratory control standard (LCS}: Refere nte standard Tru e value {TV) Sample Begin End Total Multiplier Alkalinity (MV) % RS = MV / TV K 100 number (mg CaCOJ/L) volume ml ml ml (mg c aco, /l) (acceptal>le range " 90 to UOY.) (ml) INSS \ll13'1 100 100 62 .., ~ .lo q,-:r 10.0 q-=r cn .E:P\'o Reviewed by: iiC Date reviewed: b5.\.3,~(j ] Page 59 of94 SOP C6-Revision 4- Ex h ihit C6. l
Pag Pag _ _.__of~--
--~----
Date an lyzed Analyst I: ~ ~ Alka li nity (S M 2320 B-2011) Matrix. W ter, RL = 5.0 tng CaCO /L, mples are t,tr ted to pH = 4.5 S.U.
...*..:..1.=;...__ _- - J
.......;:O~*-'-:::i T;tront normality and multiplier determ ination : -
Titrant r f rence Normality checl.. Begin nd Total ormality (/11) of HtSO* p H Fact11ror Multlpll r number stand rd nu_mber ml ml ml : (5 m l Na2C01 IC 0 .05)/ = 0.25/E " (/II x SOOOO)/ 100 ml sampl {El (accep table range .,. 0.0180
- 0 .0220) =/111< soo INR 'i3<, INS5 ILto+ 0 .l ,~.\, l~S'" 0.020'0 ) t) ,O Laboratory cont rol standard (LCS}:
I Ref ren c standard number rue value (TV) (me CaC01/l) Sam pl volume B gin ml End ml Total ml Multiph r Alkallnity (MV) (mg C;iCOJ/L)
"RS: MV /TV x 100 (acceptab e range = 90 to 1.10")
(ml)
,~.(p ~.5 l INSS \"3!
Dup IIcote somp e precfs,on:
- 100 100 'f :'i \ 0 ,0 9 -, Gf 'l o 0 /o S mple lkalinity %RPO amp! Sample 10 volume Be.gin End Total Multiptl r (mgC CO t/L) "'((5
- OJ /[(S+0)/2]} x l QO number (ml) ml ml ml {acceptab.le range = t 10 %}
m1+slJ..J s 06 .\\o.\6e:, 100 ~~-.; ~I.P L, .1 10.0 '4, I 0
..l Duplicate (OJ .J.- ~ ~.'- 3-+,".} t, .1 .J_
~ I ~L. - o5,1Cf,,1 Sample measurements:
m pl ee In Eod Total Mui
- pr r Alkal nitv Sample oumbllr Sample ID volume (m l ) ml ml ml (m g CaC01/L)
M B (TV < 2.5 ma/W Deionized wat , pH : '5. lol. s_u 100 o.o o., O .J 10.0 J.o OS - 16 .6 ~ O'tl~ aq.."l 4o.w S ."i 5"f 0S , l5 .1Af.:> I 5A '5q 1'{)'6, .\\o . \6 A- l '"'° *" ~-* P,*O-
~:5 (4' .o
\ l cP.:>'1...' .o\ Cd. CL um~ lt;, ,o- I.:\ . I 16 ,o t.., ,t ld-::,
t.z I -- -
~ J 0 '7 (fl Drna, \;l , I I 'R iJ. v?. ~ :t.
,a.(, . 11.)* \i f\ 1\1 ">W vV \ \ lf .'t- ~'4 .'+ l, :;:) <.,o o S* II.-\C I\ I z. ~~ .3.?. 2, 5",q S'\
J 3 nC.-11,d
\i uS 1'-\ . C:>1-i &
H'"'lf.,4- S &..'f'-) I 0\ I
~
5'o
.2-o ,-'J BJ., ,l.l
;i,,* * (p
~s u.2>
..:;> .'i I 7."\
"~
Sl' I t OS 11... C>~ I 2- 31.5" ~ .'+ .., .4 I 'J ) 5"'i
,.t~,,. ~ ~ .3 i+a .'+- ~. ~ ~ q { 1) <"l
\ t o s1~l-tn. , IJ A SQ~ ov ' '45.; &f'b:2.. ~~ ( 'l.) 5'1
\Pl"!< \ b . ctl I 2.. o.o ..?:1- .d ."i- ( ?. ) ~
\xo S 1~ - ~ .l 2 ~ 5.'i .3 .} P-1 l,'2.
\i C,.:,. I '-4 * {fli.. -f#,- 5&.tJ j III l \ 5.'i ~ .... -1~ (,.) SIP L ~ n, I lo c:i<. I z ~-Ip 11.c; ~ .q ( V\ 5~
'"O<;. \ {c . t>-.{ .l 3 I l.S 14.~ ~.tl L l.) Sq
\ &' o<; l~. o~ -rJA. 5&-.) f N\ VI.I \ l'+.'t- ,;} ,<:/. (.: ~)
I°'.};?. ~(,,, I£ I Y.:.. l b-() (,, Laboratory control standard (LCS}: J. 2-
- l ~ .1 2,, :0 o\ .i I 2.\ 5 <.
Ref re n c standard True. value I ' "I Beain End Total M11ltip i r Alka 111 y (MV) %RS= MV /TV lOO number (m CaC01/L) volume ml m<. ... _ (mg QC0 1/ ll (accepta ble ra nge "90 10 110%) (ml) I SS 100 100 &s1. 05.f"\,1'5 Revi w d by* ;c D te r vi w d. j05. \Cj .~ Page 60 of94 OP C6-R 1sion 4- E hlb1l C6.1
Page -25 Page a? of __...;;,.___ Analyst Date analyzed I :(, 1--=-- * - -- ~- - - ~ Matrix: Water, RL Alkalinity (SM 2320 B-2011)
=5.0 mg CaCQ3/L, Sam ples are titrated to pH =4.5 S.U .
L.._ .:;.x...*..:.. 19 .i...,.
- 1.::..
IJ _ ___. Titrant normality and multiplier de termination: Tltrant r "'~rmalitv check Begin End Total No rma lity (N) of H2SO* pH Factor or Multiplier num ber s tandard number ml ml ... ml Na,co, x 0.05)/E - 0.25/E " (N x 50000)/ 100 ml sa mp le I
~ - IC
(£) (accepta ble range= o. .- - ,01 = N x 500 INR INSS f;61., OS°ol C/ , j'g Laboratory control standard (LCS): I Reference standard num ber True val ue (TV) (mg caC03/l) Samp le volum e (ml) Begin ml End ml Tota l ml Multiplier Alkal init y (MV) (mg CaC01/ l)
% RS = MV / Tl/ X 100 (acce ptable range= 90 to 110%)
en. I INSS H,3Z Duplicate sample precision: 100 100 Sample
~a .o c<'n q ,-:r 10,0 Alkalinity Cf:}'l>(o
%RPO I Sample numbe r a~wos\c~
Sam ple ID (h\)~ volu me S} (ml) Begin t'n l
~1 .-:r End ml 3;>>.S", l'1, 1i Sa mple Begin End Total Multiplier Alka linity I Sam ple numbe r MB 1n1- ~
,.. ~
-" -- - "iieo wate r, pH =
Sa m ple ID 5.U. vo lume (ml) 100 ml ml ml (mg CaCOJ/l) 8!,(. O'S ,I <f *It
£1:;f. \I. OQ6\U ml); IOO ~'1 .'l :2,<g 9 \.0 lo.o j ,::,
j ~ I \ mOmL l!i .~ .., ~ :+ -~ .'& I~'\ l~O
\ ~n::.. \£. .CM. --fy'A~N l1....\i uv -3 ~.,{ ~-~ .;s.~ .::i ~ t :,_\ S"'h e, "C:. ,c; . ~ <;, ku d I \~ no 0.5 o .S 5 .o Ix(.)<;,,. o-z.-. l,.
\ -~'T 1.9
..,\ j....
o .'!: ...... ..t: lq
\ .k oS IC..
- crz_. 3 ..2 .'+ .~ 'l, 1.4- I'-+
\ " Cb ,s.o \ ~u.l-JccnOO Ii 0,.... l 2..5 .3 :'3 ~.o '5. l- ~\
, ? 10
\ &o<;,, .c\ I i. <=t ,Q l'"t.~ t:; .2, ->, ) '2. 10 j '
J_ l ~<JS1G\ , o\ 3 \4-.~ l~~.4 ~-1 I t.I ) ,;/"4-0 I/ [.,.-/'
..,..- ~
,,,/"
~
/
1: ( C':)t.. 05¥1-1~ Laboratory control standard (LCS): Reference standard True value (TV) Sample Be11in nd Total Multiplier Alkalinity (MV) % RS "MV / TV x 100 n umber (mg CaC0 3/ l) volume ml ml ml (mg cacO,/l) (acceptable range =- 90 to 110%) (ml) INSS 1<. 51 100 100 ~o.+ ~ ."C) <\.\o )o ,O C\l,, q <<c..<::> ~ I':) Reviewed by: Date reviewed : I Q5.tPi.\.6 Page 61 of94
<;OP rF. -RPvi~inn 4- Fxhih it r.n.1
Page ~l_v_ _ Page __...___of :3 I - Analyst ~-~ =x.....----- --1 Ha rdness (SM 2340 C-2011) Oat ana lyzed ~-t>_S_,_ _ *l_'S_ _ _~ l'2- Matrix: Water, RL"' 5.0 mg CaCOl/L ritrunt normality and mu/tlplier determination: lltr.,nt referanc Normality c.h c:I< 8 in End Total Normarll (N) of EDT,<<\ pH ~c<<iror Multipl t1um ber s t andard number ml ml ml : 0.2/ "' (N x soooo)f so ml nmple (E) (acce ptable rnnge = 0.0180
- 0.0220) : Nx 10 00 INR '&t>~ INSS IS'D'S o.o 10.t> \ 0 ,0 0.0.2. 0 0 1,o. o Laboratory control st andard {LCS):
Re f r nee st, rtdard lf'IJI! value {TV) S mple 8 gin nd Total Mull,plier Hardness (MV) % RS:MV /TV xlOO num ber (mgcaco /L) volume ml ml ml (mg CICOJ/l) ( a:eptable range = 90 to 110") {ml) INS 1(,, '~ 40 50 10. 0 4,1 105. bQ/~ I~ ,\ t.o.o -'+2. Duplicate sqmple p recision: Sample Hardn ss %RPO Sample Sample l volume B gn £nd Total Multiplier (mg caco,/l) : ((S
- D) /((S+0)/21} 11100 number (mt) ml ml ml (acceptable range = t 10" )
\3.'l s S. Ptf'll t'Jt S'o !7... 1 fr.i3...
11' '4.11 l.'1 ro.o 3'+ 0 Duplicate (0) _I ,~.'l> 15'. S 1. ":> .L 3a+ &51... -OS,1'2A'l Sample mea urements: Sample 8 in nd Totlll Multipl er Hardr.~ Sample number Sam ple ID volu me (ml) ml ml ml (mg CaCOJ/lJ MB llV < 2.5 rng/l) Deioni.ted w ater so 0 ,C) o.o o .o 1-o.o tvD S. Pln.t ,,n I I IS.5 IIp .I, I. I :1 :l. Oo,o-=r -ie, SSUJ 11,,1, 1'6.S I. 4 ~~ ()f. .If , 16 .1 UL5 ~o.5 7..,0 'iO os.o'.'.}, H!J fV\l~SW ~o.':> ~--f.,'1- "+ '2. 'i'\- OS .,o ..ieA I R_4.':t- ~.b
""1.\ 'i1.. -
os . 1o~~ei lb J -, 2..'11.'3
- J" ~ . - -
~
- . AC 1'1. ........2- 'ii+,
l~C'\~~. C \ c.d ftl Orn<< ?,?,. 0 ~ 'l.'l.. lt,1- Sa+
,L PP A-l. [)r(\(L I& C>SH:>,Oi rrum hMH
~ - I \ -- -
I ILJf l
--- 3t.2..
'"h.4
~ "+
4,15. C>
,;.. '2.
"'. te, 051 )18 i-t-130
\ R. o c. 0 ~~1.0 1v'Ast+~....i A-rn 45 O.o o,>.~ ,~}:;) q 5,~~.,1..,i rzo ~
~I
, kosoe -1..\ fun it0t d.t\ 5.~ St 100
\(r.<..l'\~-\ t Pm 5.~ ~.4-
.J .v
~ ., ~ 11.0 :l
~,
1 tosot. \C\ Pn1 l ~T - '-- , .1 11 ;0
~*" ii-'l.. \00
-.,JA !?JF N b._
\ S oso ~. ~\
IR o~o" ."1.o,.\ I z
,,,?C::,
I 11.0
\~.'B
.~ .j I &.I-.~
\,~
l.v l.4-1'd.
\& j 3 ,~.\ t8
\ k OS\ \ *
\ ~ oso8 .o'l..- 1' JA eFr.J , t-.)1 \ 2~ ,~.,
11+ 4 1":\.~ L 1-I.L. 1. 4
\ ~ o'5.o'\ .1.,S I 2 j \"1 .'1-' ,..,~- 1.'l ----- k" l aboratory control standard {LCS):
Reference ndaro '1'/'tl~lue (TV) Sample B in fnd To tal Multplier Hardn ss % RS = MV /lVx 100 number (mg CaC01/l) ml ml mgCaCO / l) (aa:eptable rang :s90to UO%) II (ml)
"~
-* r--
INSS 40 so ML z-,.,,rz .. t-Revl C!w ed by: Date reviewed: I 05. \ Z-16 Page 62 of94 SOP CG*R vision 4-Exhiblt C6.l
2 Page"""--~'--- Page ,I' of 3 aS-c,5.1;i.,1'it I Analyst ~...::es::.c.-<.,------1* Hardness (SM 2340 C-2011) Date analyzed 1:>6 .n... l~ . Matrix: Water, RL:::: 5.0 mg CaC03/L I Titrant normality and multiplier determinaUon: Tit *eference Normality check Begin End Total Normality (N} of EDTA pH Factor or Multiplier number ml ml - 0.2/E "'(N x 50000)/ SO ml sample I
.Sta n"u:u1,,1 .. ~ .. *--
- n nl80
- 0.021.0}
(El la ""' *~* = NxlOOO JNR INSS -ss.. 1:>S.\2,r"c. Laboratory control standard {LCS): I Reference standard number True value (TV) (mgCaCOJ/l) Sample volum (ml) Begin ml End ml Total ml Multiplier Hardness (MV) (mg CaC0.1/l)
% RS = MV /TV x 100 (acceptable range = 90 to 110%}
,~.+
I INSS I~\~ Duplicate sample precision: 40 50 :1,,2, 1.9 'JQ.o 2>3 'iS.~ 01,.., Sample Hardness %RPO I Sample Sample ID volume Begin End Total Multiplier (mg CaC01/L) ,. {(S
- D} /[(S+0)/21) x 100 number (ml) ml ml ml (accepiable ranee * :t 10%)
,1,, 1..0.0 s
\K.OS l \ ,\ '\ rfvA & F.iv ll\ll3 .;z5 ..11.; ~5.o t.'1 vi D
( J, Duplicate (DI .L o<.!,o c,1'4-.'=l- l.'1 I '" 1' j_. li6 eF.,l, - oS.1'2. .. i Sample measurements: Sample Begin End Total Multiplier Hardness I Sample number MB1",., L. *., ... ,.,*~, Sample ID
~ia-: ...... ~ ....._.. "Ir volume(ml) c:n ml ml ml (mg CaC01/L)
&,... 0<;;,11.ii
\R ,~.rn . <J?, PR~tm~ \ ~5 o<'-J..'t .;[~.o ~.~ I :2.., 1.,0,0
"'2..
, rOScA.~'- I 2. .2":}.o .2 q .5 .:) .s Joo I
1&-os 1\.lf
\ ~oScrt.o...\
, ie. oSc'\* "~
~ HnClf'\
J I
"w 3
J z o?9.S 3.i..s
';u..L l, 3l.5
.~.,,
'2.1~.a 3.o
?
'~
,t::>
17-Q
'1Q
_'.i:.L_ ____
,i-oS.\\,I'\
cS.-~\~ f\ In 1-lsvJ fn.m
~ 3 l
'5"'C
*',!,,. ~
~.le>
~f; ....
1-j,~ g
~.~
.y.~
°1'2 i4-oS'*~*\k e, J. I YaS 4t,.q '{.\ <f'Z...
~.,
I os-o,*d~* J ?; _L
~
0,0
... 1i.1r .1..1..1 '32. ~ lO s. <Si
- CT'.> l:::Asl--muA Anh I ~5' -+.\ ~ 11 .i+ IJ.; 1-,' q2_.
I ~c,SQ\ .1..1- I 2 1,_y. "1 ,e :;J .'O 100 14 os.11.1x J 3 't.o 11.i .Q ,?, 110
, .k uscr, .o<t I-~trru:.ri l1 ufttih' I I I* '6 13-1" Iq '1-lo
, k o~,....:: . 1.:i. I z \ ;,-:i, IS.'6 .2, I 'i4-l~OSl\. , \'1
\ f CJS<n .o \ 5f>iN..
I l 3 -
.~ n
,s.i.
\~.I 1'5,1 1q,q
- J ,2..
t,S
'h-31.,
I~ OS.O°I * ;..\ I 2. l'I.~ ~ I, 1.P L"J '.<t+ l~O~\\. \'5 J .3 .Q1 ,v ~a.s; l.q :Z,S?
, ko~oJ: . olo ~ t It 1' N'Y'ltvn I _L--
- l.?S ~'".\-.~ ..;.i 1\p La b ora t orv contra stan d ard(LCS: J Reference standard True value I'., - ,Im Bel!in End Total Mullipfier Hardness % RS=MV /TVxlOO INSS number (mg CaC03/L) volume (ml) ml (mg CaCOJ/l) (acceptable range,,,. 90 to 110%)
40 50 -~l. O:S,1'2 *18 Reviewed by: Date reviewed : ! ~-\2,..l.(3 Page 63 of94 SOP CG-Revision 4-Exhlbit C6.1
I r;::i C""?
- 11_ , -
Page 3 Page of ... I'S
~'--~
I Ana lyst J S5L- Hardness (SM 2340 C-2011} Date analyzed 1-t:> ....:~ 2,._ .-,q - - - - - i c..:..::.,::... Matrix: Water, RL = 5.0 mg CaC03/l I Titrant normality and multiplier determination: ntrant reference Normality check Begin ~, ... T.~,- Normality (N) of EOTA pH Factor or Multiplier
------..*~.9.:!JE = (N x SOOOO)/ 50 ml sample I
number standard number ml ml ml (E) (acceptable range : 0,U.J.!Slf
- u ,u ~ =N>< 1000 INR INSS BSt. b ~.,:i *\1S Laboratory control standard {LCS):
I Reference standard number True value (TV) (mg CaC03/l) Sample volume (ml) Begin ml End ml Total ml Multiplier Hardness (MV) (mg caC03/ll
% RS=MV /TV X 100 (acceptable range= 90 to 110%)
I INSS It. I~ Duplicate sample precision: 40 50 ~ ** :i, ~q.~ ;i.o -i>>.o .i+o 10'0.d:l/o Sample Hardness %RPO I Sample number Samp e ,- volume (ml) - Beg1n r--mt--~ End ml Total ml Multiplier r--- s (mg CaC03/L) = {(S
- 0) /[(S+D)/2)) ,c 100 (acceptable range = +/- 10%)
I Sample measurements: Duplicate (D) ~ l E\2.~ Sample Begin End Total Multiplier Hardness Sample number Sample ID volume (ml) ml ml ml (mg CaCOJ/l}
,,_~~ 1-. .,. .., C
*u* " --- 0!:,,\-:2 *\ \ e~u 1 .k oS , o, 'R.)U\.V'l'\ h cun 2. ~,.; 3~.'i i .\o "1.J) . O I
~'1.-
I &' OC:..1'2
- 0 \ J 3 °'"'
J.. n .-, ~.3 'g,4 I I 1-::i-t> I~
\.f o, ,'IA.. l~ 1 ~n1*N-.hn ~c:; ::c:: Sl 6~.Cf ,'1---- 1"2-0
- ,':>,,\ 1. .
I I"\.
)
./
I V
./
,,/'
/""
,,,,/
/
/
/
/
(
\...._
~ A<...,
c:6,f'l*li Laboratory control standard {LCS) : Reference standard True value (TV) Sample Begin End Total Multipli er Hardness % RS=MV /TV x 100 number (mg CaC03/L) volume ml ml ml (mg CaCOJ/l) (acceptable range = 90 to 110%) (ml) 1N$S lv I 'g 40 so 3~.9 "1-o.'l .;? ,() "t,l:>.O Y,b \~.o0 to Reviewed by: Date reviewed: !Q6./'l.,f (') Page 64 of94 SOP CG-Revision 4-Exhiblt C6.l
Page _!~ - - Page - ~ -of 2. Analyst I &sv Hardness (SM 2340 C-2011) Date analyzed b5, 1q *l'T> Matrix : Water, Rl = 5.0 mg CaCO /l Titrant normality and mu/tip/fer determination : Titrant reference Normality check Begin End Total Normality (NJ of EDTA pH Factor or Multiplier number standard number ml ml ml =0.2/E = (N x 50000)/ 50 ml sample (E) (acceptable range = 0.0180
- 0.0220) "' NX lOOO INR '&~ INSS ,s~~ o.c 10.0 10.c 0.0~t>O 1-<=>,0 Laboratory control standard {LCS}:
I Reference standard number True value (TV) (mg CaC01/L) Sample volume Begin ml End ml Total ml Multiplier Hardness (MV) (mg CaC01/l)
% RS= MV / TV x 100 (acceptable range = 90 to U.0%)
(ml) INSS \\,1\-g 40 50 10.0 \'20 .2. .o 1.Q.o i;,o .P< a.:: )00. ()~k, Duplicate sample precision: Sample Hardness %RP O Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/l) = {(S - D) /[(5+0)/21} x 100 number (ml) ml ml ml (acceptable range = +/- 10%) s os.,1,,,,1e6 VY\~W So 12.0 It, ,'2,.. ~:2.. 1-o:o <iJaf
~ Duplicate (D) J- .L 0
'"" * '2-Jo a+- .14.t.- ~a+ ef:,1.. - - b$,1Cj ,tf Sample measurements:
Sample Begin End Total MUitipiier Hardness
~--
Sample number Sample ID volume (ml) ml ml ml (mg CaC01/L) Z.~.o MB (TV< 2.5 mg/LJ DeionizC!d water 50 0 ,C> 0 .() O.o NO
~ I"\,\\
06.1e1~1t mlt-SW ..!>n .<+ .21+.1,, 4.2. '5li~ oE; 1.A.1 ea .J ~~-"' d'! i ~ -'2. ~4 ()C;. J L, \-8 A- (hj+1,.lAJ ~~ -2: :u 0 >-\-.1.. ~4-I IC b~i.""\. o\
~
(l(i(~ Dn r ..vt l:)tY'l(l.., brf"\'2.-
~:a...o
.31.1 3'1,1
,4.J_~
J.4..1
'+*'2.
_j2.
~1+
,...c. -,o-\i' A "" 1-\ c.. .u uv I 4-1.~ ~ LI- 4 .\ 2'2.
I t)~ ~\lo*\i fl\ I 2 ~5.4-J.+q *"'
,4., '2. '84 Q~
- l'I. * \.~ ~ J .3 -- o.o '-+.L A-t.1.... '1i4
\A u~1'-l. lS'2- 1.J/Jt. ~t)~ lo\ l ~'5 "+, '2.. fi.q I.~ ...... L,&
I Sc. () C:, \\ "',.l \ ,z. 5 ."I °1'1-l. I< .. . 1 ls,o
\ ll: "~\ f. u-:, .! .3 1".4- C!.o u, :'7 \ ~4 1 c. oS 1'-\. <rL 11/A SlS\11 \1,' I 't.o 10.'S 1:S ( :2 \ L,,O \ ~ OS\ lo..t>...\ I 2 t,:;,.S l~ .I ),\p I "2) t.i-i.
1.R 0~ \~.a,... l 3 l..l I l:~."l l.1.t "2. l l.4-
,~re:::.,...t .~ "l../ /),, 6&\..) lt.l T" l l~.':t 15'. "2.. 1.45" _'"\..,s l,o
\ &. O'S 1\o. Cf) I :2. ,s., I t.,,i" I. IP 2.f" L,&J.
\ R. t\C \ l2. ()'-( I 3 111,q lK:t \.S ... \ lto
\ ls I)"::, *'-l- Cl') -QA -S~t-l t ~vu I I 'I .:l, 1q.i 1.5 lDo 11'7 ')
I i ~ll..()~ l 2, l'U ~I,? , .c:; ~)- ..... l-o Latmcotory control standard {LCS}: Reference standaro- -'fro&4'.I TV) Sample Begin End Total Multiplier Hardness % RS" MV /TV x 100 number (mg caco1/l} vo ....... _ ml ml ml (mg caco1/l) (acceptable rang = 90 to 110%) (ml) INSS 40 so ~ 0 $ l'l -l'x Reviewed by: Dale reviewed : !05.l"H0 Page 65 of94 SO P CG-Revisio n 4-Exhib it C6.l
Page '2-o Page ~ of__,,:a-_ Analyst I '£1,'6c.. Ha rdn ess (SM 2340 C-2011) Matrix: Water, RL = 5.0 mg Ca(Q3/L Date analyzed ===o:s:.:,"l::.i=~======== Titrant normality ond multiplier determination: Titrani reference Normality check Begin End Total Normality {N) of EDTA pH Factor or Multiplier number stanoaro num- -,
-* ml (E) 1a -~ = 0.2/E
- 0.0180
- 0.0220)
= {N x 50000)/ SO ml sample
= N X 1000 INR INSS ---a.:.. -.IQ.IQ Laboratory control standard {LCS):
Reference standard True value (TV) Sample Begln End Total Multiplier Hardness (MV) % RS = MV /TV JI 100 number (mg Ca C01/l) volume ml ml ml (mg CaC01/l) (acceptable range"' 90 to 110%) (ml) INSS He\"S 40 50 ~,.~ ~3.3 ~ -0 1,.:,,o ~ 1o0.n°b Duplicate sample precision : Sample Hardness %RPO Sample Sample ID volume Begin End Total Multiplier (mg CaC01/L) = {(S
- OJ /((S+D)/21} x 100 number 3 (m l) ml ml , ml (acceptable range =+/- 10%)
s
\A: c6l8 .o--l tYA-SC~ I~ uv ~5 .:>~.:2. l.:>..i.i I _.c; t i-u"lo,o t...o
~ Duplicate (D) .l- ~4."b .JI.,.~ I.S l ~, ..L D
~o esl.. -os.,~.,,
Sample measurements: Sample Begin End Total Multiplier Hardness Sample number Sample ID volume (mLJ ml ml ml (mg CaCO,/l) mo \IV < .<.:, l)'lg/l) ue1on1zeo walt, C'f'>
~ os .,~ .\~
,&. l)~\c;. - U"'L- £nke.J cl l 5o ;?t,,3 :l')-.~ 0.9 'ZJO,O Ii
\kO~\ "\ * (rL. I 2. I .:n. '). <<'S .'+ , *'2., .:)&.+
\ (:-OS,)'\-cn-
~
.3 _L...
-~-
fl*) , - _ 31.o \.S ,~o
\ k K,S. C>\ Otu.!lct~~
'2 o7S .;2,z; ,4 33, 0 ~-"' ,.,_, I c;( n
,£rt:. l-:J ,{)\ . I I ob/Q 31'-"lr "+ .'+ .2.') l'Ko lf<:610\.0\ J -6 _L 3"1,o '+3 .o '-1-.o ( :i.)--- Il.:>'C>
- /
/
../"'
~
~
~
/
Laboratory control standard {LCS):
-- Qa t)~1JCl,l'il Referenc standard True value (TV) Sample Begin End Total Multiplier Hardness % RS= MV / TV x 100 number (mg cacoi/L) volume ml ml ml (mg CaC01/L) (acceptable range = 90 to 110")
I INSS H.i<~ 40 (ml) so '-t-3.o '-fS.o ,Jn 1,,o.o ~ lot> n °/n Reviewed by: Date reviewed : I();, I'\, w' I Page 66 of94 SOP CG-Revision 4-Exhibit C6.1
'equ yah uclear Plant Biomonit ring M l - 22. _QJ Appendix D Referenc o *icant e t and ontrol hart Page 67 of94
Pimephales promelas Chronic Reference Toxicant Control Chart EnvlfonmtotaJ J .Jtln9 SoH.nton s. In c. Source: In-house Culture 1.2 Control Limits(+/- 2 Standard Deviations) 1.0 0.8 * ---
--.- ~.-----. * =--.- -.~*~---. * - -*- ~ .~- .--. - -** - - 0.6 0.4 U'\
u N 1.2 ro Warn ing Limit s
"'O I 1.0 . .............. ......... , ................ ***** ............... ...... .. ... .... ........... ........................... . " -**~-- ........ **-
0.8 ____,____.*----~=---------*--.;..---4*-~----------*----------.:'.'---~* 0.6 .. .--**-**-**-* __ .. _. ... - .. ....--**-** 0.4 Test date
- 7-day IC25 = 25% inhibition concentration . An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc) .
Cent ral Tendency (mean logarithmic IC25 converted to anti-logarithmic values) - - - Control Limits (mean logarithmic 1<;5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic 1Cz5 +/- 2 coefficent of variations converted to anti-logarithmic values) USEPA Warning Limits (mean logarithmic IC25 +/- SA. 75 converted to anti-logarithmic values. 1 SA.75 = 75 h percentile of CVs reported nationally by USEPA) Page 68 of94
Pimephafes prome/as iCD E*1i111011mt ft1, 1 ft'IIIOtJ lohHlont. kt Chronic Reference Toxicant Control Chart Source: In-house Culture
~ \,I Log10 Conversion Anti-logarithmic Values (c/L KCI)
Ti t numbe r Test date 7-day IC25 Laboratory Calculated CV 75th Percentile CV ToxCal Determination 7-day ICu CT 5 CT Control Limits warning Limits waming Limits (g/L KCI) CT
- lS CT + lS CT - 2CV CT + 2CV CT . SA.75 CT +SA]~
1 12-06-16 0.8303 -0.0808 -0.1423 00433 0.7206 0 5904 0.8795 0.5399 0.9411 0.4468 1.0009 2 01-10-17 0.7066 -0.1508 -0 1433 0.0432 0 7189 0.5892 0.8773 0.5384 0 9392 0.4457 0.9921 3 02-07-17 0.7052 -0.1517 -0.1421 0.0426 0.7209 0.5924 0.8772 0.5426 0 9378 0.4469 0.9948 4 03-07-17 0.7620 -0.1181 -0.1362 0.0365 0.7308 0.6178 0.8646 0 5761 0.9139 0.4531 1.0086 5 04-04-17 0.7043 -0.1523 *0.1392 0 0351 0.7258 0.6174 0 8531 0.5765 0.9012 0.4500 1.0015 6 05-02-17 0.6740 -0.1714 -0.1371 0.0316 0.7292 0.6305 O.S434 0.5939 0,8858 0.4521 1.0063 7 OS-16-17 0.6906 0.1608 -0.1382 0.0320 0.7275 0.6278 0.8431 0.5904 08864 0.4511 1.0040 8 06-06-17 0.7367 -0.1327" -0.1393 0.0314 0.7256 0.6280 0 8384 0.5911 0.8810 0.4499 10013 9 07-11-17 0.7519 -0.1238 -0.1393 0.0314 0.7257 0.6280 0.8385 0.5911 0.8811 0.4499 1.0014 10 08-08-17 0.6829 0.1656 -0 1379 0.0294 0.7280 0.6357 0.8336 0.6012 0.8731 0.4513 10046 11 09-12-17 0.7271 O.l 38'1 *0.1371 0.0292 0.7294 0.6377 0.8342 0.6037 0.8730 0 4522 1 .0065 12 10-03-17 0.7024 -0.1534 -0.1382 0.0293 0.7274 0.6355 0.8326 0.6011 0.8720 0 4510 1.0038 13 11-07-17 0.6666 0.176] *0.1'121 0.0289 0.7210 0.6310 0.8238 0.5961 0.8636 0.4470 0.9949 14 U-07-17 0.6847 0.1645 -0.1404 0.0265 0.7237 0 6406 0.8175 0.6089 0.8533 0.4487 0.9987 15 12-05-17 0.7191 -0.1432 *0.1424 0.0251 0.7205 0.6418 0.8089 0.6112 0.8432 0.4467 0.9943 16 01-09-18 0.7574 -0.1207 -0.1397 0.0244 0.7249 0.6478 0.8113 0.6185 0.844] 0.4495 1.0004 17 02-06-18 0.7951 -0.0996 0.1377 0.0260 0.7283 0.6461 0.8211 0.6154 0 8557 0.4516 1.0051 18 03-06-18 0.7002 -0.1548 -0.1387 0.0263 0 7266 0 6437 0.8201 0.6126 0.8552 0.4505 1.0027 19 04-03-18 0.6973 -0.1566 -0.1411 0.0256 07225 0.6423 0.8128 0.6115 0.8474 0.4480 0.9971 20 05-08-18 0.7204 -0.1424 -0.1429 0.0243 0 7196 0.6435 0.8048 0 6138 0.8380 0.4462 0.9931 Not/:!: 7-diy ICu ~ 2S"l. 111h1b1tion concenv a tlon An estlrnatloo of The potassium t hlor,de concentrallon thal would cause a 25?. reduction In P,meplloles growth (calcu lated using ToXCalc) CT = Centra l tend!!>ncy of the IC,, values Cont rol Ll m, ts = Mean loganthmlt IC 1, +/- 2 standard deviations converted to anto-logauthm,c v.1lues Warning Limits = Mean logarithmic ICu +/- 2CV or s,.,s converted to antHoganthmlc va lues. Sus = Sta ndard dev,a11on corre~pondin@ ,o the 7S'" percentlle of CVs r eport.eel nationally by USEPA (SAP-
- O 38)
C\I "' Coert,clent of 11<1**at1on
Pimepha/es promelas Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture 1.25 QI 1.00 .s:::. CV
~0 ro....> .... 0.75 --- -- --- --- * --- * *
- CJ 0 :~
ro
*--- - ----*- --'*!:. .----. ---* -------.*: :.--=-------- * ........C: ......C:
0 u E QI) 0.50 __ ~- - 0.25 USE PA Minimum Accep ance Criteria (> 0.25 mg per surviving larvae) ~ 0 20 USEPA Maximum Coefficient of Variation Guidance Criteria(< 20%) C .c 0
- .::Ill ~
"i: ro (!) 0
..._--- ~-- --- -
0 ....C u 0 C 0 10
*-----*-4 *
- ---------~. * *
=---1-~.
ClJ
- a
- EClJ u
0
-0 0 ----- -----* -
._~_ __.._.......__.....__~--~- ~ ~ --J---1-_~ - ~ - ~ ~ - - ~ - ~ - ~ ~ -........---__.
USEPA Upper PMSD Bound Acceptance Criteria (< 30%) 30 USEPA Lower PMSD Bound 0 0 0 V') 20 (>12%) ~
- a. *
- 10
- Control Growth, Coefficient of Variation (CV) or Percent M inimum Significant Difference (PMSD)
PMSD is the percent minimum significant difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sens1t1v1ty of the test method and is not a minimum acceptance criteria . Central Tendency (mean Control Growth, 0/ or PMSD) 95% Confidence Interval (mean Control Growth, CV or PMSD +/- 2 Standard Deviations)
Pimephales promelas Chronic Reference Toxicant Test ing, Test Acceptability Criteria Source: In-house Culture i: ToxCal Determination Control Growth Control Growth CV Test PMSD Control Control Growth Test (mi/1M1il IIIVH) (") 11'1 Test number Test date Survival Mean CV MSD PMSD CT 95% Confidence Interva l CT 95% Confidence Interval CT 95% Confidence In terval 11'1 (mg/1n,tiai luv**I (!,o) (1'1 CT - 25 CT-t25 CT- ZS CH2S CT- 25 CT* ZS 12-06-16 100 0.617 5.6 O.U67 18.9 0599 0413 0786 57 0.0 11. 4 11 .2 4.6 l7 8 2 01-10-17 100 0.587 7 .3 0.0629 10.7 0.600 0414 07 6 57 0.0 11 3 11 2 4.6 17 7 3 02-07-17 100 0.579 lLS 0.07Sl 13.0 0.591 0.420 0 763 62 0.3 120 11 .4 4.8 17 9 4 03-07 -17 100 0.638 6.2 0.0662 10.4 0.590 0421 0759 62 0.4 12 0 11.2 4.7 177 5 04-04 -17 100 0.620 s.s 0.0919 14.8 0.5 l 0.422 761 6.3 0.6 11 9 11.3. 4.7 17.9 6 05-02-17 100 0.729 S.6 0.0792 10.9 0 596 0 416 0776 6.2 0.6 118 11.d 5.0 17.8 7 05-16-17 JOO 0.593 2.8 0.0521 8.8 0.603 0.434 0.771 6.0 02 lL8 11 4 5.0 17.8 8 06-06-17 100 0.608 7 ,2 0.0542 8.9 0,601 0 434 0 768 5.8 0.7 10.8 11.0 5.2 16 8 9 07-11-17 100 0.713 3 0.0589 8.3 0.609 0 43 0.781 56 0.5 10 7 10.8 49 16 8 10 08-08-17 100 0.638 7 .9 0.0791 12.4 0 .6 16 0 451 0781 59 LO 10.8 11.0 S.1 16.9 l 09-12-17 100 0.631 S.6 0.0562 8.9 0626 0 489 0.764 5.9 10 10 8 10.8 5.0 16 S 12 10-03-17 100 0.658 4.4 0.0838 12.7 0637 0525 0.7 48 60 I2 10.7 11.0 5.4 16 6 13 11-07-17 100 0.738 3.9 0.0641 8 .7 0.645 0 52 0.761 57 l I 10.n 10.8 5.2 16 4 14 11-07-17 100 0.727 0.8 0.0 80 8.Q 0.642 0 535 0.749 s. .6 10.0 10.S s.o 16 l 15 11-0S-1-7 100 0.676 2..3 0-0607 9.0 0.647 0545 0.750 5.2 0.4 10.0 10,6 5.0 16 l 16 01-09-18 100 0.6U 8..3 O.O?S 12..3 0 .643 543 0.7 4 54 05 10.4 10.8 5.0 16 17 02-06-18 100 0.772 8 ,4 0.0115 10.6 0.651 0 S3 0.765 56 05 t0.7 10.6 5.0 16 2 1 03* 06-18 100 0.810 4.7 0.0640 7.9 0660 0.526 0.794 56 05 10.7 106 4.9 16.3 19 04-03-18 100 0,743 6.1 0.0836 11.3 0.668 0S 4 0.802 s 0 10.'7 10.7 5.1 16 3 20 05-08-.18 100 0.576 11.9 0.0516 9.0 0.663 0.523 0.803 5,6 07 10.S 10.8 S.3 163 Note~ Cof'trol Sur.Iva! w USEP m1n1m1..11 n tut a<<,pc b1111vot1tt1o1 g .~1u4v.,vt t C()ntrol Me:an Growth -: VSE.P~ rmn,murn tell I c*ptab111tv crner1a ~ O 5 mc/su'Vi f'! 1 rwe CV:. co,mciiml o II r, oon rorconuot aro\vth USE.PA m .m\Jm Cl/ u,dance. cottna f oercentt 1e c. 2 MSO =- Mff'\1"lum , .fan d1fftt nc~ PMSO .a P *fttnt mintmum ** n1ftant d1fTtU!nt.e P SO ls a me;nure of ten pr b.siOn Th PMSO s tht "'"-" De-rceN ddf , ,nee tt~twe1:.n I controt nd tr atm, ot tf..1t un t>e decl eQ .s.ta11srlca y J n1f+c.a"t t w ,~ ,h11.tent to 1atv tru Lowor PMSO boo"4 ltrm>nN bv u;EP 110' ~*c*n , l > IN ft lowtr PM'iO bound t~ 1e_se,nu pra r to tne stn111,viw o* tht test n, _ t'tod rld ,i not a mtnimun,
- 1pDn cri , r,a Uoper P1v1SD bound *<< p11*1<* u "'"' dot rm,nea by U!.EPI\ 190' ptlttnt*if, < 30'!(
CT= C*~tr I t ndtnc-, of lhc 11ow1h , CV at PMSO **'
-; St.1ndard di:oY,*t lan of ,he g,owth V or J:.M~ lloilue
Page I of6 £11110:,nm,~uJ Ttillnq Soludo,u, tl'lc. Pota sium hloride Chronic Reference Toxicant Test (EPA-822-R-02-013 Method 1000.0) Species: Pimepha/es promelas PpKCICR Test Number: 39 DU11tio11 nrenm*ntion informntio11: Commeuts: KCI Stock rNSS number: INSS \lo(S Stock preparation : 50 g KCI/L: Dissolve 50 a KCI in 1-L Milli*Q water. Dilulion prep (mg/L) JOO 4::iO 600 750 900 1050 Stock volume (ml) 9 13.5 18 22.5 27 3I
- Diluent volume (ml) 1491 1486.5 1482 ]477.5 1473 1468.-
Total volume (m~) l::iOO 1500 1500 1500 1500 l:iOO Test onmnism i11formnlio11: Test informntio11: Or!?.anism source: In-house culture Randomizing template: \..\~ (\ u,..,l(,. Age: < 24-hours old Incubator number and 1_& shelf location: Spawn dale : 0~
- O'\..-\& Artemia CHM number: CHM984 Hat ~h dares and times: OS-;),*\ 'ti: \ ~~'C)
-rb Dry!11g i11for111atio11 for weight os-o.f- \~ 0~00 deter111i11ntio11:
Transfer vessel infonnation: pH = ,.g o s.u. Date / Time in oven: ~-\S*lt ms<; Temperature = '"I.\.S oc Initial oven temperature: lot>. (. Average transfer volume: < 0.25 mL Date/ Time out of oven: O':>*l\.*\4 cnss Final oven temperature: bO. C.. Total dryi ng time: 1..'-\- ~o..:i.tS Daily feeding mul reuewnf information: Day D:itr: :t.forn ini;: feedin g ! Afternoon feeding T est initiation, i*enew;,I, MHSW or tcrminn lion batch u ed T ime Annlyst T ime Analyst Ti me Analyst 0 05-08-18 oc; \c;
.v\ \\LS ,ll o,:iS x\ OS*~**e i\
( 05-09-18 t>l.:,n o \,f \ £..00 ,11 0£00 11 OS*cr'l..*IJ h
\J -
2 05- 10-18 \L,00 " 0 ~QC) "i os-o ~- \ i' f, 1"11.-.r\O y( \t u* . 3 05-1 1- 18 ~ - Q\c,OC)
., \1.00 ,I{
O~<Y) At. N;.-CJl* *K & 4 OS. ! 8 O\o~ O \{ \.1..~ 0 w l')A ~t. ll{ I\<:.* rt"\-\ of-5 .' " - ' 6 05-13-18 05-1 4- 18 Olo~O Ql... 00 k\
.~ l "1. "\.(\
\ "1..0C) o~~o O&bb l,(
~
M,-<n-1} l"l,-cn-lR 7 \J o, 05-15-18 0\)~S, R Co11trol illformatio11: Acccptnncc critcrin S1111111w1J1of test emlooi11ts:
% Mortalitv: D1, s:20% 7-day LCso 85& . '1 Average weight per initial larvae: o .SlL NOEC looo Average weight per surviving larvae: u.s,6 ~ 0.2:5 mg/lan*nc LOEC 1<:. O ChV lo 10- g IC2s ,1.0-11 Page 72 of94 SOP AT2l- ExhibitAT21.I, revision 11 14
Page 2 of6 l Species: Pimephales promelas PpKCICR Test Number: 39 Survival n11d Gro wth Data Day Control 300 mg KCI/L 450 m~ KCI/L A B C D E F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 JO JO l to It) 10 10 IO (t) co IO ! \) { () ID ID 2
/0 10 l t) l C) 10 I \.J l\) tO l 1) IQ 10 lO 3
£t) IQ \0 It) IO I<:) l C) tu 10 (C) tO ( i) 4 LC /0 l t) It:> 10 /C) fC) to lb I \:) LC) ID 5 l C) ( \)
/0 10 IC) 10 10 CQ LC) l C) ( \.'.:) (0 6
to IO IQ ro It) I t) < a lD l C) (C) LC) {() 7
/0 , C) ( \) 10 'C) 10 ' t) (~ ( \) I{) I () LO A ~ Pnn wcigllt (mg}
Trny color code:; '..furc;rr 8CUO 14.'?3 15.~t?. /{ 91 15.3~ 15.45 15.N Jw.51 JtO~ 15.(lo 15.J~ 15.~J. Anni *sl: : fS Date: Q4 * :;t£1- IIf:
,~.Cf I D = l'nn + Lanae weight (mg)
Annlysr: TS ~C.4-5 J.0.75 Jf,O~ J \. a5 ~ .all J.J.. 19 l3.l.3 ~ .(j5 al.39 cl.I. ~.? ~O.*'f1 o< \. ;i~
'.;:: le: ..!l5:.=..J:1- j ~ ,.
C
- Lnrvnc weigh t (mg) = 8 - ,\
llnml cnlculn ted. s.1o'l. s .s~ I.,. n S.1 \ "'-1~ i. :~ o 1.o.S'\ 1.,.'\~ $.1 ~ I.. ,\'\ S. foS <,,.~:i Ann lyst: ~ Weight per initia l number of larvae (mg)
"' C / Initial number of larvae q..."?
linnd calcnlnlcd.
~
rv ~/>'}
" ~ ' ~ "°'
0
'>:)
I~ Y}
\() \.D ",
A.4;
'D
~
~
":> 4)
' 0 o* 0 Cf o* er Average weight per initial Pcrccnl number of larvae (ow) rctluclion from conlrol o. 'S,i\, !
- o. lo l,A - \~-~,. Cl. S<\ & * ~- & 7.
(%) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm u unusually mall, lg= unusually large, d&r = decanted and returned, w - wounded . Comment: Page 73 of94 SOP AT21 - Exhibit AT21.I , revision 11 14
Page 3 of6 _. £n.v1 ranmtntlt l'f!11Uli9Sokrt on\ lnc. pecie : Pimeplta/e5 promelas PpKCICR Test Nu mber: 39 Survival a11d Growth Data Day 600m1 KCI/L 750 mg KCI/L 900 m: K CI/L I 0 M N 0 p Q R s T u V w X 10 10 10 10 10 JO 10 10 10 10 10 10
- 1 I to IQ / IJ ID I ti IO ID 10 &zrA.. C\ '~ g~
2 q1 A. g
°' '"'
ro I0 l () lD ID IQ I () '\ t\. 8 3 10 t C) 1t> lQ 10 &.,\ I() ~D 1'~ q q g 4
/0 l C) I l) 10 '\J,A. g 10 u!
l C) \ r'" tA ~
~
5 ,<( IQ IQ { C:::, le) &>\ ~ G\ t\ ~J.. 8 -,"' .j ,_____
,-,z J
6 ID / () 10 °I '>. fs lo\.
.u(
8 s
,c.l
~\... s \J..
5u\. 7 ,a 10 ,o q -, ,"'.. 'l i °' .~
& ,t.( '{ 'i l{ *c:\ *.f" A = Pn n weig ht (mg)
Trny co lor code:: £acec:,-\: Anolys t: TS
~ g ll.!)
/4.wO 15. 'l3 fll. ~~ 16.JI 15.SCo /5.<?4 15.~0 J5.G2 15.J.5 14. 7.3 15.05 15. J(j Dn tc: Ql\
- 21~ - 18:
(J = Pn n + L:1 rvnc weigh! (mg) Anni *sl: Gnrc: a.5::- 1::Z- 1~ IS ~0.70 3.1.50 /1.0. (pJ. J.O. ?i ~ .01 l<l,7q ao,3q 19. <t~.. I?,17 11.io \1.Sq IR JL.\ C
- Lnrvnc weight ( mg) "' 8 -
1,,_10 S . \o'"l '- * ~\:, 5.~, l.f, \~ ~ ."IS 4,\:t:\'-\ *V~o .. . er., ,.,-.\1 , .s'\ "2. s.-t llnml cnlc11l:1ml. 1\11 11lyst: e~ Wei hi per lnil i~ l numbcr of lnrvne (mg)
/ ln itin l number of lnrvnc A
.t, ..,ob :::i-(>.~ ~o lln n!I cnlculnl d.
.$> cf '6
"'.) '-,~
. o* 0 . o*
~'\!'
I\, J,.." I\, ~~
'\; "v
':,;).
Annlyst:
~ o*
\g 0 o* 0 ""'. 0 o* o* Cf ()° A vcrngc weig hI per initia l Percent num ber of lnrvnc (mg) rctl uc ti ou 0. 'S'\~ -:,.01 . 0. \.\~'\ "2.\.l.'1 7. o. l.S1... S.lo* ~ 1.
fro m control (%) Commei1t codes: c = clear, d = dead, fg = fu ngus k = killed, rn ... missing, sk = sick, sm = unusual ly small, lg = unusually large, d&r = decanted and returned, w = wounded. Comments: Page 74 of94 SOP AT2 1- Exhibit AT21.1 , revision I J-01-1 4
~ P- C-:J I *
- Page 4 of6
_ EmJtonnmu,11 felting Sohn kins. lnc. Species: Pimepha/es promelns PpKCICR TesL Number: 39 Survival am/ Growth Data Day 1050 m KCI/L l 0 y z AA BB 10 10 10 10 sSc.\ I lo
"'"' <o '\c:A. (.,,\.(
2 ~ I,:, s s *"- 3 ls ~ 'S s 4
\o ~ ~*~ c.\ ,A.
5 3
~.A..
~
u\.
~
I" '-\ 6
- IA Id,,
- > ~ '- 3 A= Pnn weight (mg) 7 -i'"- \ 1:>J..
\ '" \ ul.
fo<~t ca<ee.n Trny color code:: nalyst: :f:$ 14. q() 15.Ji /5.07 (5..3~ Dnte: Q4*.:2!:!- I~ 13
- Pnn + Lnrvne weight (mg)
Analyst: TS Dute: 05.- IJ- l I{ JG>. 11 1s.n 15. 73 15. CJC C "" Larvnc weight (mg) A lfand en lculn 1e(I.
\. 'l."1 o .S\o o.,1o 0 .,; .,
Ann i , i: Xi Weight per initial number of lnrvn (mg)
- C / lnitinl number of lnrvac A
f-fnnd calculnted. {" " 0 i::t' ~
'O o*
t-1 Analyst: t,. ~>' o* 0 1\ vcrnge weight per i!1i1inl Percent 1111111be1* of lnrvnc (mg) rcduclion from control ().011 Slo,, ?. (%) Comment codes: c = clear, d = dead, fg. = fungus, k = killed, m = missing sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded . Co111111e11ts: Page 75 of94 OP AT21 6xhibil AT21.1 , revision I 1 14
~
CQ Pimepho/es pfome/os Chronic Refere nce To,cicant Test CD EPA-821-R-02-013, Method 1000.0 0) 0 tr1"11~t1Q.t°TP\,1noSntrt.t1:llfl_1 .lirl( Qua lity Control
"'II
- Verificatio n of Da ta Entry, Calcu lat ions, and Statistical Analyses Test number:
Tosi do101* l~*V08*15, 2018 39
,.~ittfe:" '"""l l(Q) ii..~.... Wt,al~eJ llftal'""""'*~**' A tc ,.111M1(n:tlfTICI . . ,.". \llrVlt LliMC .,.1fh1 fm:d :c Wflr,1 /t.ff.-...t--. ........, .. ,Jft\l/.Jrll.t\->*11( C..:ff1a..c,.tl:l!Wl11il1i.111 W*iJI)' / r,--tllll 1W*011t, oJ fl,,1.-,.1ww,l*IO,J t,jlun ....:til/h*lll_. CHff>dffllM-.,~i.;., .... ,.,... t ,.itadt011'11P""1 t~*J a
1'~"9..... ......
'ilN'A" -1~1(11\ll ~ nw,r,i-~elf4,..,-..("lfl IIMMfOfl,;an,*t*jnlO 1,f'VM lffl&I PILl,,,ltltrofl..'Wt. M (.ff ltpi
_..., t,,, r) A 10 10 14 91 10.45 S.61 0561 0.5&2 B 10 10 ISH 10 75 SSl 0.5Sl 0.57i 49 O.S53 100.0 0.576 4.9 Not 1pp.1*c.1ble Cot1trol C 10 10 14 92 21 09 6.17 0617 0617 0 10 10 15,34 ll OS 5.11 0571 O.S71 [ 10 10 IS 45 ll.24 6.79 0.679 0 679 f 10 10 l!i.89 l l 19 & 30 0630 0.630 4.?
'300 0.1164 4' 100.0 0.564 *ISJ Ii 10 10 1659 23.13 6.SA o .6SJ 06~4 H 10 10 14.0 l 2095 UJ 0.69! 0693 I J.O 10 1566 1139 5.73 O.S7! 0513 I 10 10 15~~4 21.43 619 0619 0 619 o.s,a
-150 0.598 H O,S65 100.0 s.£ *3.8
~ 10 10 IS22 20.87 5.65 0 56S l LO 10 lo.91 ll.2.4 6.33 0.633 0 633 M 10 10 I0.60 20.70 6.10 0.610 0 610 N 10 10 IS.Bl ll.SO S.67 0 567 0.(08 49 0567 6,2 -3,.0 600 '7.5 0.593 0 10 10 14-26 10..61 616 0 6'6 0.6JS p 10 9 15.JI 20.79 5.5& 0.610 11.SSS Q 10 7 1586 20.01 4 15 0 593 0.415 R 10 7 1580 1979 J.95 0 S64 0.39~
750 s 10 8 15.40 20.34 U4 0.618 0.57! 60 0.49* 75.0 0.431 u 74.7 r 10 8 LH2 19.91 4.30 053! 0.430 u 10
- 15.25 17.71 :uz 0630 0!52
!IOO w
V 10 10
~
103 IS.OS 17.20 l7.St l .47 1.54 061S 0.6lS 0 6l!l 0.2A7 OJS4 40 0 0.2S2 1.3 56.3 X y 10 10
*2 l!i.70 14 90 1824 15.17 2..s4 1 27 0635 0635 0.15*
0127 1050 z 10 l 1S 22 IS7! OS6 O.S50 0606 SI 0056 12.S 0.077 44.4 80.1 AA 10 l 1507 15.75 06& 0,660 0.06& OB to l IS 3l 15.!IO 0.57 0.570 0057 Ounnett's MSD nhte: O.OS1 MSD
- M1nwnum SSnif,c.ant 01ft.,*nce PMSD* 9.0 PMSO ~ Pe:rcen, Mtnlmum S1cnlflc1nt Offil!",:1 nc.t PMSO ft* n,e,nure c,r ten prec.,.ski11 The PMSD 1s the ffilf'lm\t.un perc*nt dlfftrr.nc.e batwt.'<<" 1he controt and u u tmont t h.t c;1n ~ dfffiued stlti\UC..tllV!'lfllfc.-lnt in .1 whole efflu.nl 101fKllV t~t
'--"' PMSD bound dftl!fm,ood bV USfPA 00th r,e<<ennlel
- 12%
Upr,e, PM;D bound deu,.........d bv USEP~ 190th Plf<OftUlel
- SO%
Lower ai11d up~, f'MSD boundJ. were determined from tht 10th and !Kl1h a,enentJlo, r-upec:uv,ly. of ,MSO dilta hom EPA .i WET h'n.erlaboro1tofv ""'!abtl*rt Study t\JSfPA 2.0011; USEPA, 'JOO lb) The lowor PMSO l>:Jl.ffld ~p,~or\U a practle.ll 'llm1t lo tht .sens.ti\n\v of 1M tttl method *.,d 1J.11ot i IT"lfnlmurt" uupttrc* cnt*rlJ. llSEPAi ?OOh, 2C01b. ftt\al Rep0rc lntf-rlabo,ato,; Viar,ab,,,ty S.t\idyof EPA Sh0n*tt1ffl Chro"ic 1nd Aei.te Whote URu~r,t lor1e1ty ltst MHhod\, V~omes l 1nd 2*App1nd1a ~PA*!i-2!*1*01--004 ~nd EPA*82l*B*Ol-00S. US Erw,ronmt.ntJI Proiac.hon ~ek\! CM'lt1"11111I OH
....r..-
-r
~* .-
Statistical Analyses Larval Fish Growth an d Survl vaf T est-7 Day Survival Start Dal
- 518!2018 Test ID* PpKCICR Sampl ID, REF-RerTo ,cant End Date 5115/2018 Lab ID ETS-Envir Teslong Sol Sample Type KCL-Po1as um chlol\d Sample Date Protocol
- FWCHR*EPA-821,R-02-013 Te t Species PP-P,mephal s promel s Comments Co nc-m IL 2 3 4 O*Control 1 0000 1.0000 I 0000 1 0000 300 1 0000 1.0000 I 0000 1 0000 450 1 0000 1 0000 1 0000 1 0000 600 1 0000 1.0000 l 0000 0 9000 750 07000 0 7000 0.6000 0.8000 900 0.4000 04000 0.4000 0 .4000 1050 0 2000 0 1000 0 1000 0. 1000 Tra nsform : Arc sin sguare Root Rank 1-Talled Numbe r To tal Cone-mg IL Mean N-Mean Mean Mi n Ma x CV~/. N Sum Critica l Rese Num ber 0-Conlrol .0000 .0000 141 20 1.4120 1 4 120 0000 4 0 40 300 1 0000 1 0000 1 4120 1.4120 1,120 0.000 4 16 00 10 00 0 40 450 1.0000 0000 I 4120 14120 1 4120 0.000 4 18.00 10.00 0 40 600 0 9750 09750 1.3713 1.2490 1 4120 5.942 4 16.00 10.00 1 40 "750 0 7500 07500 1 0492 0 .9912 t 1071 6.363 4 10 00 10.00 10 40
*900 04000 04000 06847 0 .6847 0 6847 0000 ,4 t 0.00 10.00 24 40
' 1050 0 1250 0.1250 0.3572 0 3218 0.4636 19 861 4 10.00 10.00 35 40 Au xllla~ Tests S tallstlc Critfcal Skew Kun Shap,ro-Wilk'!. Test 1nd1caies non-normal drstnbouon (p < = O 01) 0 87142 0 B96 -0 2B93 2 16 Egus,,tt or ,:mance can!\Ot be con ,rmed H~ot hesls T estp -tail . o.osi NOEC L OEC ChV TU Steel's Many-One Rank Test 600 750 670 82 Treatments vs 0-Conlrol Maximu m Llkellhood-P ro b ft Param ater Valu e SE 95% Fid ucial Lim its Control Chl *Sg Criti cal P-,alu e Mu Sigma lter Slope 12 59B1 1 67874 9.30775 ,s 8884 0 o 015,3 g 48773 0.99929 2 9314 O 0793B 3 lntercep *31 93 4 91886 -41 571 -22.289 TSCR 10 Poi nt eco, Pro b lS m g /L 9S% Fiducia! U m l 2674 558 141 477 111 613 98 09 r EC05 3.355 632.177 562.953 679.848 01 EC10 3 7 18 675.58 614 . 186 71 8 592 0 7 EC15 3.984 706.535 650 845 746 S63 l:C20 4.158 732 145 66 1.06 770 106 ll: 0 C
EC25 4 326 754 855 707 .608 791 .441 8_ 05 EC40 ecso 4,747 815 253 775.803 851-554 5.000 853.891 816 6 893.659 i 04 EC60 5 253 894 36 856 198 941 279 03 EC75 5 674 985.921 920 361 1032 97 02 EC80 5842 995.882 945.635 1073.49 EC85 6 036 1031 9B 975 257 1123.55 OI EC90 6 262 1079.27 1013 04 1 190.82 00 EC95 66 5 1153 36 1070.6 1299.41 lO 100 1000 10000 EC99 7326 1306.36 11 B5.27 1533.44 Oose mg/L D ose-Respon se Pl ot 0.9 08 _ 07
~~ 08
~ 05 2l o4
.... OJ 02 0,
0
~
I Page 77of94
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date. 518/201 B Test to PpKCICR Sampl 10. REF-R rToxlcani End Oa t 5115/2018 Lab ID ETS-Envir. Tes ting So S mple Type. KCL-Po1ass1um clilonde Sample Dale: P otocol* FWCHR*EPA-82 1-R-02-013 Test Species* PP-P1mephales promelas Comments. conc-m IL 1 2 3 4 0-Conlrol 0.5620 0 .5530 06170 0 .5710 300 06790 0.6300 0 6540 0.6930 450 0.5730 0 6190 0 S650 0.6330 600 0.6100 0 .5670 06360 0.5580 750 0
- 150 0 3950 0 4940 0.4300 900 0 2520 02470 0 .2 40 0.2540 1050 0 1270 00560 00660 0 0570 Trans rorm : Untransformed 1-Talled lsoto nlc Co n c- m!i!/L Moan N-Mean Mean Min Max CV% N t.-Stal Crltlcal M SD Mean N-Mean 0-Control 0 5758 1 0000 0.5758 0 .5530 0 6170 4.944 4 0.6199 I 0000 300 0 6640 1 1533 0.6640 0 6300 0.6930 4190 4 -3 919 2.290 0.0516 0 6199 1 0000 450 0 5975 1.0378 0.5975 0.5850 0.6330 5.617 4 -0.966 .290 0.0516 0.5975 0 96!9 600 05928 1 0295 0 5928 0 5580 0 6360 6 190 4 -0 755 2 290 0.0516 0.5928 0 9562 750 04335 0 7529 0 4335 0 .3950 0 4940 9 874 d 0 4335 06993 900 0.2.S 8 0.4373 0.251 0.2470 0 2540 1.312 0 2518 0 4061 1050 0.0765 0.1329 00765 0 .0560 0.1270 44..400 4 0 0765 O 123d Awdllary Tests St.alllsUc Crittc:al Skew Kurt Shapiro-W,ll('s Test ,ndicales normal d1stnbu1ion (p > 0 01 ) 0 89619 0 .844 0 24992 - 1 s;sa Elartlell's Test 1nd1cates !:9Ual vanances (e a O 96) 0 211117 1 .3449 H)'.~lhesls Tes-qt-ta ll. 0,05! NOEC LOEC Ch V TU MSOu MSDe MSB MSE F- Prob di Ou nelt's Test 600 >600 0.05157 0 .08957 0 00602 0 00101 0 01008 3 12 Treatments vs D-Control Llne~r Interpolation (200 Resamples)
Point m!!IL so 95% CLjExe) Skew
!COS 603 64 78 83 281 .16 639 87 -0 8198 IC10 632.84 15.23 592 01 66671 26811 IC1 662 03 1 96 623.04 701 48 0 1775 10 IC20 691 .22 12.82 65800 736 94 0 4492 Q9 IC25 72042 14 65 686.2S 77090 0 4973 08 IC40 800 82 9 95 769 45 927 68 -0. 1471 07 IC50 851 98 5 42 83565 8 7 63 -0. 1673
. 06 0.5
"'C: 0<1
&. 03 a: 02 01 00 01
*02
-0 .3 0 500 woo 1500 Dose mg/L Oose-Respon e P lot 0.8 01 06 1-ta I, 0.05 lev I s=
J 05 or s gn,ficance 2 C:, 04 i;'
....O 03 0.2 0 1 *-.
0 l! T
§ 0
Page 78 of94
l Q Page 5 of6 _ Envtronmrnr~l tHtlng So'u1fot1t. Jnc.. l Species: Pimepha/es promelas PpKCICR Test Number: 39 Daily Cltemistry: Day (A.nolysl identilicd for cnch dai, performed pH D.O. and conductiv11v mcasurc111cn1s onlv.) 0 J 2 A nalyst J-{~ H..! it.ff "-' i>,.., HJ Concentration Parameter ' pl-I (S.U.) -:/.9~ ~.!2. ":'.f."e '+.u,q 9.01 -:;.-=15 DO (mg.IL) -=,.-=I 1 *°? "=/ . e 1.4A ~. ~ -=f.-'t Conductivity 31::/ ?.lo 3~Y -
*~,:A
(~1mhos/c111) I', CONTROL
- Atk3linity (me. CaCOJ/L)
S41 sq ~-'. "-**-
*lfardn g<o (mgCaCO.i/L) &~ "~
"Temper:iture (°C) ""\.-\ ' \:, '1.---\. b "\...tl
- 1 -..-t., '"\.'\ . .t "\..'"'*"\
pH (S.U.) 8.03 '::/.[JI 7/-"'!'1 'l.\l~ '().01-- 7--=!3 DO {,~*g/L) -:/.~ -:/. fo 7{.11 ~o '1-,r °1-'f 300 mg KCI/L Co11d11cli\'i(y (11mhos/cm) 8&2
.,_~.,
85Co 04; .I
*Tem 1>erature (*C) ""L'-1 .\., "'t.i.\-1 "\..o..l* 3, 1.-\-'\ L.-.\-la pH (S.U.) 8.oz '1.32. ?."11 'll4 I 0.0'-l '=1---=F"i DO (mg/LJ !ts 1 .e;- '1-<:f '\..0 '+,')- -=,.~
450 mg KCI/L Cond uctivity (u01hos/c111) //Oo / I Jo toeo "Tempcr:iturc (°C) '"\..'-\. le, "\..'-4 , ,_ "'\.<{ , f '1..1.\. s I.I.\.'\ - d-b pH (S IJ.) e.oz. ':{:~(3 -=1.. <f(,, ~.S1-" 'o.~ -=,.:::,5 DO (m£/L) -=i-B 7:.5 =1-"l u, p, 't.~ '"7-., 600 mg KCI/L Co nductivicy (1tmhos/cm) 1380 1350 \'?,50
- Temperature ( C) 0
'"\>I. lo 1..1.{ -'-l '\.I.{*1 i,'{.S "1.'\,, ""\..'-\- '1..-
t>H (S.U.) 8.0"2. -:,. -:f. (J 71-'lS ~.'5\4 ao5 ':f,bt::t 750 mg KCI/L DO (mg/L) "':lq +*5 e.o IP,8 ~-6 "?s Con du cl ivity {1tmhos/cm) /020 /b3o . 1~20 .
"'Temperature (oC) r..-l* b 1..-t. 6 1.1{.1 "'I..<\., s 'bt . .f '-"'* (.,
pH (S.U.) '8-CPO -:/.80 7 . "'15 ~."'ll J1 8.Dla ':/. ":lo DO (mg/L) -:,.q 7#.5 f:3. 1 'ho ',t.G( "7,5 900 mg KCI/L Conductivity *, (J unhos/cm) l'8"'ro lct.oo l~O "Temperatu r e ( C) 0
).'-\ . 6 1..'{. 3 1.~ .* "\.'\* lo 1..'{ , t 'l..c.t,S pl-I (S.U.) '8,01 ? .TB ":/:.?s 'l.c;,")- e.o'l -:,_? {;,
DO (mg/L) ? . "i i-~ e., lP.e e.o 7-9 1050 mg KCII L Conductivity (umhos/c:m) "Z l~o 2180 AtOfD "Temper:iture (0 C) '1'-\-b "l..~
- S 1.'-(.1 "1.'t . s i.L\ , t "l.~.s In itia l Final Initia l Fin al Initial fl'inal
*Tcmpcrn1ures pcrfom1cd m ll1c time oftest i11itintion, renewal or tcnninmion by the analyst 1dentllicd in 1h Dail Renewa l lnfom1:1tio111able loc ltd 011 Page I
*alinity and hardness pcrfonncd by the mmlyst identified on che bench sheet specific for each analysis and transcribed 10 this bench shccl by: _ _ _
~ d . .Hftlll SOP AT2I - Ex.hibitAT21.1 revi sion 11 -01*14 Page 79 of94
l Page 6 of6 l fnvitot1nutnt,I TaUt,q SoluUont.. l..c. l pecic *: Pimep/wles promela PpKCICR Test Number: 39 Day (Analys l identified for each day, pcrfonncd pH, D.O. and conductivity measurements only,) 3 4 5 6 Analyst HS l'J;L., ~, N V,..; ,J..) i,l, rt.5 ' Concentration Parameter pH (S.U.) -::,..CJ.If +:so 1-,G\S t,Vq Ai~ '+.~ eoo ":?.ff3 DO (nig/L) ? .';f +*"I :}.~ ':\-0 +.".l. '~.1- +.8 -., *'i Conductivity
?Jci)
CONTROL (µmhos/cm)
*Alkalinity (mg CnCO:;/L)
*Hnrdncs (mg CaC0 3'L)
.305
'\....Ji.
~~
' ~
~"""'
l&>O
&'-\
&01..,
*~
.. ..- J
.. '\_,.,,,t .
" Temperature '
(OC) '"\.~., "1..-\. 'S "\...L\,, "'\..'-l.b 1.1.\. \, "'1.'-',4 i.,"\. f "'L'-\.'-\ pH (S.U.) 8.oLJ "'1."J,..:l ?.~ 'l.~~ 0.o'-1 ~!JO E?)D't- 7/.135 DO (111g/L) -={,.3 +,'l ~-q ~.o '\..u 'l..u, "l,1'1 -:[.4 300 m" K 1/ L Con<l uctivily Cvmhos/c111) Bt.18 151- 9-t~ e2.;
*Tempcrntur (oC)
'"\_I..\ ,g "'-'-'*3 "l_'\. q '\.,'-( . !) .,_~.\a 1,..-.\ .s i.'-l'\ "1.'-\..b pH (S.U.) (i.03 +:1--\., y_r,::; ".}.~ 8/)4 l)...ultJ e>.e>S -:is,,
DO (nig/L} 1,8 ~.g ~.o u?.'l /}.".} ':\. V, ~~ "-:f..fo 450 mg KCI/L Conducth*lly (~1mhos/cm) /110 11\D ll\O lo~
*Temperature
("C) 1.-'\. ~ .,_'\A 1A1 '1...'\ .t.o ""l!-\-\c, "-4 .s 1...\.'\ 1..'-\-~ pH (S.U.) 8 -03 "1-, tA 'i.o'5 /l.,Yl 0 .0~ ~.lJ~ a.os- -=l-83 DO (mg/L) '":{. fj "l, .-:i- ~.o 'lo 41' ':\:+ ,~~ -is 600 mg KCI /L Co nduc1h<i1 * (umhos/cm) /31JD 13qo \660 1'3140 "Tempernture "\..~.~ "\.,~-1 -i_'-(.S '-L~-b i....\..lo °l"I,'\ ,'-I. !, (OC) "1...Y-* pH (S.U.) fj'.o3 ~.1-0 ~.o'5 1 .se, a.o, ~-~'l A,'lu "":/-'Bo 00 (mg/L) f.9 "+,If ~.D (I."'.}- '-t8 ~ r-t,'1 t*S Conductivity 750 mg KCI/L (µ mhos/cm) /~10 ~'430 ll4'f0 I\o:Z.O 11-'fem pcrnture "l..4."' 1.tl .1 \.'l* s "'\...4-, '1...\. ~ 1..'-t* '\ -i:-tS (OC) pH (S.U.) 1.~ B.oz.
*°' 1-:~1 'il,o 'i- /)i,,'2, ,.°" ~J~2 8.03 1,. ts>l 900 mg f CI/L DO (mg/L)
Contluctivity (111nhos/c111) t-9
/360
~ ..4:>
"*o ltf3o l:\.o '},a
('lSO
'.\.lo ~A ll\QJ
-={.5 "Temperature
(*C) 1..f& i..~\. ~ l. ~. (I L~-1 t.'\-1 1.4-1 "\.-{ .'i 1,t.\.b pH (S.U.} 8.02. 1.~l ~.~ 1..~3 ~,() 1' 'l.(~(4 e.>.oY ~ - Bs-DO (mg/L) ConductiYI tJ.o +.~ <?.o t".).1, l.t.e ~.'l- e>.O '::/.5 10-0 mg J Cl/ (u mhos/cm) 2.ISO o()OO a1,o '-"o
- Temperature 1.~.f 1.'-\* ) 1\(,f L.~-~ 1..~-1 1,.L{.3 "t<i. D ,c.{.1 (oC)
Ini tia l Final Initial Pinal Initial Final Lnitial Final
****~~'fl: eratures performed at 1hc lime of test initiation. renewal or tcm1ination by 1h analyst identified in the Daily Rcncwnl lnfon11otion table locatc~n Page I.
,,....
- h ity and hardness pcrfonncd by the nnnlyst identified on the bench sheet specilic for each analysis and 1ronscribed to this bench shcc1 by:
~ Page 80 of 94 SOP ATI I - Exhibit AT2 l . I, revision 11-01-14
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Erwlfonmenc I Testfflg SoluOans, ln<.. Source: In-house Culture 1.10 .----.---,---.---.---r--..-----.,----,---,-- -r---r---,----,,----,---.---r--...--.,----,,----,---, 1.08 --- .-- Control Limits (+/- 2 Standard Deviations) 1.06
. .=------*--~--~------. -un, z 1.04 ..J -u ao Ill N
C"O 1.15 .,.,. * ** -* *** r*** **** ** * *** *~*r** * .. * ........... , * .. * . * ************* * * ****** ***** ******t*tr***
"C Warn ing Limits I
" 1.10
** .. *~-- . --*-**- *'"' . **- **-** .. .*
1.05 .. **-'* **-*-**-** **~**-**--*** 1.00
.................. , .... ..... .. ........... ......... , ************ ~*****~******* -****** . ************* *** ******
Test date
- 7-day IC25 = 25% inhibition concentration . An estimation of the sodium chloride concentration which would cause a 25% reduction in Ceriodophnia reproduction (calculated uslngToxCalc) .
Centra l Tendency (mean logarithmic I~ converted to anti-logarithmic values) Control Limits (mean logarithmic 1Ci5 +/- 2 standard deviations converted to anti-logarithmic values) Laboratory Warning Limits (mean logarithmic I~+/- 2 coefficent of variations converted to anti-logarithmic values)
- *, * * * * *
- USE PA Warning Limits (mean logarithmic 1Ci5 +/- S11_10 converted to anti-logarithmic values, 1
S"- 10 = 10 h percentile of CVs reported nationally by USE PA) Page 81 of94
Ceriodaphnia dubia hronic Reference Toxicant Control Chart In< Source : In-house Cult ure co l og10 Conversi on Anti-logarithm1C Values (g/L NaCl) Test number Test date 7-day IC1s Labor.110,y Calculated CV 10th Percentile CV Toxcal Dl!termmauon 7-day 1(25 CT s CT Control Limits Warning limits Warni ng Limits (g/L NaCl) CT - 2S CTt2S CT -2CV CT+ 2CV CT. SA.10 CT+ SA.1U 1 01-10-17 1.0723 0,0303 0.0276 0.0031 l 0656 1.0506 1.0808 t.0515 1 .0796 0.9803 1.1508 2 02-07-17 1.0641 0.0270 0.0278 0 0029 1.0660 1.0517 1.0804 1.05.26 1.0794 0.9807 1.1513 3 03-07-17 1.0641 0.0270 0.0276 0.0029 1.0657 1.0516 1.0800 1.0524 1.0789 0.9804 1.1509 4 03-07-17 1.0614 0 .0259 0.0276 0 0029 1 .0656 1.0515 1.0800 1.0524 1.0789 0.9804 1.1509 5 04-04-17 1.0654 0.0275 0.0274 0.0028 1.0652 1.0516 1.0791 1.0524 1.0781 0.9800 1.1505 6 05-02 -17 1.0691 0.0290 0.0275 0 0028 1.0654 1.0516 1.0794 1.0525 1.0783 0.9802 1.1506 7 06-06-17 1.0614 0.0259 0.0276 0.0027 1.0657 1 0524 1.0791 t.0532 1.0781 0.9804 1.1509 8 06-06-17 1.0518 0.0219 0.0275 0.0029 1.0653 1.0511 1.0798 1.0520 1.0787 0.9801 1.1506 9 07 17 1.0631 0.0266 0.0275 0 0029 1.0654 1.0512 10798 1.0520 1.0787 0.9802 l.1506 10 08-08-17 1.0810 0.0338 0.0277 0 0032 1.0660 1.0502 1.0820 1.0512 1.0807 0.9807 1.1513 11 09-12-17 1.0527 0.0223 0.0274 0.0034 1.0650 1.0435 1.0818 1.01195 1.0805 0.9798 1.1502 12 10-03-17 1.0540 0.0228 0.0272 0.0035 1.0646 1.0474 1.0821 1.0484 10807 0.9794 1.1497 13 11-07-17 1.0528 0.02l3 0.0270 0.0037 l 0642 1.0463 1.0824 1.0473 1.0810 0.9790 1.11193 14 12-05-17 1.0469 0 0199 0.0269 0.0039 1.0638 1.0449 1.0832 1.0460 1.0817 0.9787 1.1489 15 01-09-18 1.0826 0.0345 0.027d 0.0042 1.0651 1.0446 1.0859 1.0458 1.0843 0.9799 1.1503 16 02-06-18 1,0500 0.0212 0.0270 0.0044 1.0642 10427 1.0862 1.0440 1.0844 0.9791 1.1494 17 03-06-18 1.0697 0.0293 0.0272 0.0045 10646 1.0430 1.0867 1.0443 1.0849 0.9794 1.1498 18 04-03-18 1.0835 0.0348 0.0273 0 0047 1.0649 1.042 4 1.0880 1 0437 1.0861 0.9797 1.1501 19 04-03-18 1.0827 0.0345 0.0274 0.0048 1.0651 1.0420 1.0888 1.0434 1.0869 0.9799 1.1503 20 05-08-18 1.0822 0.0343 0.0275 0.0049 1.0655 1.0415 1 0899 1.0430 1.0879 0.9802 1.1507 Note
- 7-day ICu = 25% lnhlb1clon concentrauon An ut,matlon of the sodium chloride c.oncentrc111on that would c:.use a 25% reduc1,on ,n c,modophn10 reproduction (calculnted using ToMCalc)
CT ~ Co?JHral lendency or the ICi~ values S = Standarct deviation of the IC,s values. Control Li mits = Mean logarithrolc IC>i :r 2 ~tandard dev,allon~ convened to antl-logarlthmtc values Warning Li mits = Mean logaTlthmlc IC11 r 2CV ors. 16 corwened ro anli-logarothn"c values. 1 S~ 10 ~ Stal'ldard cle,v,anon corresponding to the 10 ' pe,cenUle of CVS reported nationally by USEPA IS~ 111 : 0.08) cv = Coefficient of vanatton.
Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Organism Source: In-house Culture
- ,e C _
0 QJ 35 - ._.. __ *- .-.- -- . .,.~-,-----*
- iij
]~
- i
~~
er: E 30 C 25 0.._ C.
...o_
C
~
VI 0 20 USEPA Minimum Acceptance Criteria(> 15 offspring per surviving female) u 15 10 l C C: 0 USEPA Maximum Coefficient of Variation Guidance Criteria(< 42%), NCDEQ Maximum (<40%) 0 u IU
- J "C 30 IU
...0
~
....>0 QI 0:: 20
... 0......
C: QI
- u 0C: 10
- E11/ u...
u 0
-0 0
USEPA Upper PMSD Bound Acceptance Criteria(< 47%) 40 USEPA 30 Lower PMSD Bound (>13%) 20
- Control Reproduction, Coefficient of Variation (CV) or Percent M inimum Significant Differen ce (PMSD)
PMSD is the percent minimum significa nt difference between the control and treatment that can be declared statistically significant. The lower PMSD bound represents a practical limit to the sensitivity of the test method and Is not a minimum accept ance criteria. Central Tendency (mean Control Reproduction, CV or PMSD) 95% Confidence Interval (mean Control Reproduction, CV or PMSD +/- 2 Standard Deviations)
Ceriodaphnia dubia Chronic Reference Toxicant Testing, Test Acceptability Criteria Source: In-house Culture f""' ToxCal Oetermln;itlon Control Reproduction Control Reproduction CV Test PMSD Test number Tes t date Control Surviva l Control Reproduction Mean CV MSD Test PMSO CT tolfspn11g/fem,1te) 95')(, Confidence Interval CT
'"')
95% Confid nee Interval CT ('i.) 95% Confidence Interval 11') (of1Spring/fon1ale) I'*) 1%) CT- 2S CT+2S CT 25 CT+2S CT-2S CT+2.S 1 01-10-17 100 30.5 3.2 1.524 5.0 30.4 27.7 33.0 4.8 2,3 7.4 5.8 4.3 7.3 2 02-07 -1 7 100 27.9 8.5 1 .895 6.8 30.2 27 .4 33.0 S.l 2.2 8.0 5.9 4.4 7.4 3 03-07-17 100 29.7 3.9 1.530 5.2 30.2 27.4 3.0 5.1 2.2 8.0 5.8 4.3 7.4 03-07-17 100 29.9 5.3 1.918 6.4 30.2 27.4 331 5.1 2.2 8.0 59 43 74 s 04-04-17 100 30.3 4.9 1 .924 6.3 30.2 27.4 29 5.1 2.2 7.9 58 43 7.3 6 05-02-17 100 31.0 5.9 L894 6.1 30.2 27.4 32 9 5.1 2.3 8.0 58 4.3 7.3 7 06-06-l7 100 30.7 4.9 1 .968 6.4 30.2 27.4 32. S.2 2.5 7.9 59 .6 7 .3 8 06-06-17 100 33.1 4 .6 2.0SO 6.2 30.2 27.3 332 S.l 2.5 7.7 59 .6 7.3 9 07-11-17 100 31.0 4 .8 1.661 5.4 30.2 27.3 33.l 5.0 2.5 7.6 5.9 4.6 7.3 10 08-08-17 100 28.7 4.7 1.668 S.8 30.1 27.2 33.l 5.0 2.4 7.5 59 4.6 7.3 11 09-12-17 100 30.3 6.8 2.191 7.2 30.2 27.3 331 5.0 2.4 7.7 6.0 4.5 7.4 12 10-03-17 100 32.2 3.5 1.926 6.0 30.2 27 .3 331 4.9 2.2 7.7 60 4.6 7.4 13 11-07-17 100 32.3 7.4 2.215 6.9 30.2 27. 33.2 5 l 2.1 8.0 6l 11.7 7.5 14 12-05-17 100 28.6 6.0 2.344 8.2 :30.3 27.4 33 1 52 2.5 8.0 6.2 4.5 7.9 15 01-09-18 100 29.7 4.5 2.015 6,8 30.l 27.11 32 8 5.2 2.5 8.0 63 .7 7 .9 1 02-06-18 100 29.3 5.6 *1 .824 6.Z 301 27.4 32 8 5.2 2.S 79 6.2 4.7 7.8 17 03-06-18 100 30.6 6.8 1.995 6.5 30.1 27 .4 32 8 5.2 2.4 8,0 6.2 4.7 7.8 18 04,03-18 100 28.0 4 .5 1.729 6.2 30.l 27.3 32 9 5.3 2.6 7.9 6.2 4.7 7.8 19 04-03-18 100 1.8 5.5 2.108 6.6 30.2 27 .4 3:31 5.3 2.7 7.9 6.3 .7 7.8 20 05-08-18 100 30.3 5.4 2.172 7.2 30.3 27 .5 33.l 5.3 2.7 8.0 6.4 <1.9 7.8 No te Control Su rvi,al = US EPA r111n1mun1" l acccplabtllrv cn1e,1a i'! 80% survival Control M an Rep,oducUon = USEPA n11nrmum t Sl ace ptab11t1v cr,te"a l lS orrmnng/survtv1ng remal CV = Coeffcien ol vana uon tor control renrod,,ction USE PA ma ,mum cv gwdance en e, ,a (90° pe,centile) < 2 NCDEQ ma ,mum CVacceptance cnttna < 0%. MSO
- M1n,mum s,gn,l1can1 dlffc, nee.
PMSD = , cent ml"'n1um ,gnlf,cant difference. PMSO ,s a measure or test prttr ,on The PMSO" the mrn,111um pe,cen1 d,lrcrence beiween tie con1101 and 1tea1n,ent that canoed clared 1.,nlstlcal vs, nof,cant ,n a wl>ole effl ut.n to *Cl!Y test Lower PMSO bountJ dete1"1tned by U EPA ( 10' percenrile) > 13%. The low r PMSD bound rep~s n1,
- llta tocal hmlt to t~e Sf!nsit1v,ty of rhe tesl metl>od and 15 not~ nll!lllt\Um acceot nci? ttl~na.
pptr PM O bound acceprance ulteua d~1e1mtn d bv USfPA (90 perrenrne) <-47. CT
- Central tenue11cy ol tilt reproduwon, CV or PMS0values 5 .. Stanoard d ylatlon of the rep,oduct,on, CV or PM O., lues
0 - C-:J-:: Page I o 6 n odium Chloride Chronic Reference To icant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaplmia dttbia CdNaCJCR #: 215 Di/11tio11 prepm*atio11 informatiou: Comme11ls: NaCl Stock INSS number: lNSS 11.,, , Stock preparation: I00 g NaCI/L: Dissolve 50 I.? NaCl in 500 rnL Milli*O water. Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 IS 11 Diluent volume (ml.} 149 1 1488 1485 1482 1479 Total volwne (mL) 1500 1500 1500 1500 1500 Test orgm1ism source i11fomwtio11: Test iuformntion: Organism age, I < 24-hours old Random izinit template color Ke.l) Dote pnd times orgonisms were born between: I oS*bl *It' o~~o~ 0'\Clb lncubnlor number and shelf lr,:-at1on: "2...l1. Culture boaro: ci:-u,-\r.6.. Replicate number: I 1 I 1 I
- I s I 6 I ' I s I 9 I 10 YWTbntch:
CullUrc board cup number. '-l , I f- I '\ I '~, I~ I q I l f I *11. 1~ o'\*H,-t e Transfer vessel in fo m1ation: PH= , .'\o s.u. Temperature= i.'\ ,' ,*c Sele11n:r1ru111 batch: Average transfer volu111c (mL}. < 0.25 mL o~~ - l8 Daily reuewnl iuformatiou: Day Date Tes t in itia tion a nd feedin g, MHSW Analyst renewal a nd fe eding, or lrn tch used
. t'!rn1ination time 0 05-08-18 A(
Of-\\\ OS-~*lf f:>.. I 05-09-18 (')Q~1 OS-~;~ ~ kt 2 05 18 " o~'S.S OC.:.-m,.t~ ~ KC
\J 3 05 18 OhS 1- OS~ *l<! & Y1 4 05- 12-18 o'\-i.i OS *U'\
- l r ti 5 05 18 oll\1-~ OS-Cf\*\~ 1(
6 05 18 o~S1- O~* O"'\* Lr/' \1 7 05 18 OR \"L- \( Co11trol information : Acccptoncc criteria Summarv of tesl eudpoi11ts:
% of Male Adults: Ol S 20% 7-da y LCso "} , t./oo
% Adults having 3'6 Broods: I I"\(\ 1 ~ 80% NO EC 1000
% Mortal ity: ()7. S20% LO EC Ii.OD Mean Offspring/Female: 3o., ~ I5.0 ortspring/fcmolc ChV \O(\ $. $
%CV: S-~ 7. < 40.0 % ICzs 1042. 2.
Page 85 of94 SOP AT l4 - Exhibit ATl4. l , revision I 1-01-1 4
l Page 2 of 6 pecies: Ceriodaplmia dubia CdNaCICR #: 215 CONTROL Survival a11d Reproduction Dain Replicate number Day 1 2 3 4 s 6 7 8 9 10 J Young produced 0 D 0 a C D 0 () 0 0 Adult mortality L_ \..- '- L \__..
'--- '-- L '-- L 1 Voung produced ('\ (J 0 n 0 n 0 0 0 [)
Adu ll mortality
'- '- \...... L- '-- L.. '-- L.. L... L..
J Young produced 0 t'\ C\ 0 0 D 0 0 0 0 Adult mortnlity L '- '- L- \.._. \_..
'- '-- \__.. '--
4 Young produccu ~ ~ ~ '-\ '-\ ~ '-\ "--\ c; ~ Adult mortality L \...- L. L L L '- \....- '- '-
- \'oung producctl I\ \":) \t_ IC)
,,_ \0 I\.) I\ l \ \\
Adult morlnllty L '-- '-- L. '-- L '- L \..._ G Young produced 0 6 l) 0 (') 0 {') (') 0 0
<lull mortality '-
'- '-- '- \ '- '- '-- '-- '--
7 You ng produce(] 1<::. \\ \~ \\.c, I\, tg ,s \\ \ '-\ \ c; To tal rom12 produced
-z...'\ :>~ ""1.. 'i 2)0 ~L. :>\ "2°'. ~"1.... ~c 1-~ "" Finni Adult Morlnlity - C. . '--
L. '- '- '-- '- '- '- \. X for Jrd Broods y 'x. '¥. "')('... ')(.. r-- 'i<:'.: )(_ x.. 'K. Note: Adult mono lit {L = live. D =dead), SB = sp lit brood (sin°le brood split between two days). CO= carry over (offspring carried over with :tdult during transfer). Co11ce11lrnlio11:
% Mortality: I Cl Mean Offspring/Female: I .::?)o.':l 600 mg aCI/L S11n1ival and Reproduction Dain Dar I 2 3 4 Rcnlicnlc number 5 Ci 7 8 ') JO I Young produced 0 () () 0 /) a () 6 0 0 Adult mortality \... \..._ L. L L '-- L.. '-- '- L 2 You ng produced f"'I 0 n 0 0 0 0 () 0 0 Adult morr:ility
'- \... '- L L L L L '-- L J Vo1111g prollnccd {') 0 0 0 0 () 0 C) 0 a 4
Adult mort:ility Young produced ~ l s L \.._.
'--\
L
.... ~
L '-
"-\
L
" s L '-
~
L
~
5 G Adult mortnllty You ng produced Adu lt mortality Voong produced 0 L 0
\\
\.._
0 L
\ "'L
\.._.
r,..
*~
0
\_
L 6 L 10 l~ 0 0 IQ L 10
\__
0 0 11
\.._
dull mortolity \...
,g
\..._
,s L
\\
L
\q
\. L '-
II ,,
'- \....
,5
~, '""
7 Young produced 1$ Totnl youn produced llo
- )'-\ ~, ~, ~~ ~ 31- .3"L ~t> "'l.~
Finni Adult 1\lorlnlity L L L '-- \...... \.... L '- \..... L. Note: l\dult mortality (L = ltve, D = dead), SB= spilt brood (smgle brood splil be Iween two days), CO= carry over (offspring cnrricd over with adult during trans fer) . Couceutration:
% Mortality: 07 .
Mean Offspring/Female: ~\.b
% Reduction from ContTOI: - '*\.'~ 7_
Page 86 of94 SOP ATl4 - Exhibit AT14. I, revision 11 14
a - "" ~ Page 3 of6 Species: Ceriodaplmia dubia CdNaCICR #: !!..= 800 m g NaCl/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I You ng produc d 0 0 a a D 0 D l'I (\ (') Adult morlallty \..- \.._ L \._ \_
'- \.._ \,__ L.. \_
? Young produced n 0 0 0 0 0 0 (") ri n Adult morlality \..... \._
'-- '-- '-- '- \.._ '-- L. '--
3 Young produced (") 0 t) 0 D i\ D 0 0 D
\._
Atlult mortality I_.
'-- '- '-- \._
l 4 Young produced Atlull morrnlity
~
_""1.,
'-{
\_
~
.s
'- L
~ ~
~
~
L
'-\.
- Young produced l\ lo \0 \Q I-,_ I\ \ '"l.. I~ I C) ,,_
Adult mortality
'- '-- '-- '- '-- '-- '-- '- \..... '-
G Young produced 0 a 0 0 0 ('I 0 0 0 0 Adult mortnlily L \..._
'- '- '- '- L L '-- L-l& cs \ '-\ \ l.,
7 Young produced Tolnl ou ug produced
- A l&
"':)-,_
\ \o
- P "'b:>
t
~L- . t...<\
\ I.:,
~"'L 1 l.o
~ \ :)\._
-- "" \_ '- '-- L \_ '-- -'-
Finni A<iuh !\lorr:iliry '-- '- Note: dull mortality (L = live, D = dead} SB= splil brood (si ngle brood split between Lwo days}. CO= carry over (offspring carried over with aduh during lrnnsfer) Co11ce11tmtio11:
% Mortality: r'i'1.
Mean Oftspring/Female: ~,.-,
% Reduction from Conu*ol: -'-\. b 1.
1000 ma . . . aCl/L Survival anti Reproduction Data Rcoficn te number O~y 1 2 3 4 5 G 7 8 9 10 I Young J1rod111:cd C () 0 0 ('\ (') () n 0 0 Adult mortnlity L '-- L L l_ '-- L L.. I._ L 2 Young produced 0 0 0 0 () {) 0 (') () 0
/\dull morr:ili&y \_ L- L L '- L \_ L L L.
3 Young prod uced (') (') () 0 () (\ ('i (', (\ n Adult morrnlity '- '-- '--- '-- \...- '-- \._.. L- \..- '-
~ Young produced '.\ 5 ~ s ~\ '-\ '-'- ~ ~ ~
Adult morlal ily '- L L '- L- \._ L L L '-- 5 Young produced \l ,o lQ I\ IC) I "'"L \ "L \ "1.. ,o 10 Adult morrnlily L L '- '-- '- L- '-- '- \..... '- G Young Jlroduccd 0 0 0 0 0 0 () 0 0 0 7 Adu lt morfnlity Young produced \~ L L
*'-l I \.I ,,
\._ L I lo ,-,
L tS L.. l1 L
\ C\
\._
L Total young produced
~~ "'2-'\ '"2., ""!)~ °3t) ':)"'.) '3 \ ~1... :?) "t. ~o Final Adult Mort~lily '- '- '-- '- '-- \_ L \...... L '--
Note: Adull monahty (L= hvc, D = dead}, SB =spht brood (s mglc brood sphl between two days}. CO= carry over (offspnng carried over with adult during &ransrer) Co11ce11lratio11:
% Mortality: Ol Mean Offspring/Female: 3 1.0
% Reducrion fiom Control : - 1. -l 7.
Page 87 of94 S P ATl4 - Exh ibit AT14 . l revision 11-01-14
Page 4 of6 pecie : Ceriodaplmia dubin CdNaCICR#: 215 1200 mg !'la CJ/L Slll'Vll'tt
. I WllIR eprotIuctro11 DI (t {I Replicate number Day I 2 3 4 5 6 7 8 9 10 I Young produced 01 D () t"i () 0 0 C) 0 r, Adull 111orl11llty L L
'-- '- L \....... '- ~ \.- \._
2 Young produced 0 a 0 0 c'\ 0 0
.__ 0 0 0 Adult mortnlily '- L '- L- L \...__ '-- '- L Young produced n () 0 0 0 0 0 0 0 n Adull mortnlit)' '- ' - '- '-- L '-- L L L.. L
~ Young produced :> '"'.:> ~ '--4. L. -,_ \ :::> ~ \
Adull rnortnlily
'- ' - '- '-- L '-- '-- ' - '- L
- 5 Young produced Adult mortnllty t:
\
~
~
\o tn
'\
'- '-- '°'-
G Young produced n 0 0 D 0 0 0 0 0 ()
/\dull mortnlity L
\.... '- \....... '- '- '- L,_
7 Young produced g () b 0 D 0 -'\ 0 ~ D To lnl youn
- produced
\ :> ,s \,
Finni Adult i\lort:1litr
\°-- \0
'- L ; \,._.
\ ""L Is.
\ "'"'L
\1-
'- \._
\
Nolt!: Adult mort:ilil * (L = live. D
- dead). SB = split brood (single brood split bcLwccn tw dny }, CO c carry over (olTspring corned over with adulL dnnng tmnsfcr).
Co11ce11trntio11: 1. n .. s
% Reduction from Control: ., 7.
1400 ma -
..,, . a 1/L Survival mu/ Reproductio11 Data Rcnlicnte number Dny J 2 J 4 5 6 7 8 9 10 I You ng produced n r'J () 0 0 f) 0 /" ) ('"\ 0 Adult mortality L- '-- L- L L L L '-- L. L 2 You11g pl'oduccd ('} () 0 0 0 0 () 0 0 0 Adulr mortality L '- '- L,_ L- L ~ L. L L J \louug produced () /') n 0 11) 0 (\ n (') ()
Adull mortality L L L L. L. L '- L. L L
-4 Young produced \. "L \ \ ~ \ "'L. \. \ \
Adnll morl:ilfry L L L. L L. L l L '- L
- Young protlnccd fl 0 2. 0 0 () c) L\. ""L 0 Ad ult morlnlity \ \_ L \.._ L- L '- L L L 6 Young produced (") 0 0 0 D () r, 0 n ()
Adult mortality L '- '-- L L L '- L L 7 Yo_ung protluccd () .,_ (') 0 0 0 0 0 0 () Toln l you ng produced
\ 1-l 3> \ ~ \ £... s ~ \
final Adult Mortnlity \_ L L. L.. L '- '-- L. '-- L Nole. Adult mor1nli1y (L
- live D
- dc3d), SB"" split brood (si11glc brood plit bctw en two dnys). CO .., carry over {offspring carried over with adllh during trnn fer}
Co11ce1tlratio11:
% Mortality: 07.
Menn Offspring/Female: 2 .~
% Reducrion fi*om Control : '\'2. . \1.
SOP Tl4 -ExhibitATl4 . I, revision l l-01-14 Page 88 of94
~
CQ C1) Verification of Ceriodaphnia Reproduction Totals 00 co Envlronn1 an1111TC1stlng Solution s, Inc. 0
,-.. Control 1000 mg NaCI/L f Dav 1 2 3 4 Replicate number s 6 7 8 9 10 Total Dav 1 2 3 4 Replicate number s 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 3 3 4 4 3 4 4 5 3 36 4 4 5 3 s 4 4 4 3 3 3 38 s 11 l3 12 10 12 10 10 11 11 11 111 s 11 10 10 11 10 12 12 12 10 10 lOS 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 15 17 13 16 16 18 15 l7 14 15 156 7 18 14 14 17 16 17 15 17 19 17 164 Total 29 33 28 30 32 31 29 32 30 29 303 Total 33 29 27 33 30 33 31 32 32 30 310 600 mg NaCI/L 1200 mg NaCI/L Repllcilte number Repllcate number Day Total Oay Total 1 2 3 4 s 6 7 8 9 10 l 2 3 4 s 6 7 8 9 10 l 0 0 0 0 0 0 0 0 0 0 0 l 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 5 4 4 3 4 3 5 3 3 37 4 3 3 2 4 2 2 1 .3 3 1 24 s 12 11 t2 u 13 10 13 10 10 11 114 s 8 7 s 8 6 10 7 9 s 6 71 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 18 15 15 17 19 16 17 17 15 165 7 8 0 6 0 0 0 7 0 9 0 30 Tot al 31 34 31 31 33 33 32 32 30 29 316 Total 19 10 13 12 8 12 1S 12 l7 7 125 800 mg NaCI/L 1400 mg NaCI/L Replicate number Replicate number Day Total Day Total 1 2 3 4 s 6 7 8 9 10 l 2 3 4 s 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 s 3 4 5 s 3 3 3 5 4 40 4 l 2 1 1 3 1 2 l 1 1 14 5 11 10 10 10 12 11 12 13 10 12 111 s 0 0 2 0 0 0 0 4 2 0 8 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 19 16 18 15 18 14 16 16 16 166 7 0 2 0 0 0 0 0 0 0 0 2 Tot.ii 34 32 30 33 32 32 29 32 31 32 317 Total l 4 3 l 3 l 2 5 3 1 24
'l! CQ CD co C) Ceriodaphnio dubia Chronic Refe rence Toxicant Test 0 EPA-821-R-02-013, Method 1002.0 f Envlro nm@nta l T@sllng So lu llo1,., Inc. Qua lity Cont rol Verifi cation of Data Entry, Calculations, and Statistica l Analyses Test number: Cd Na OCR 11215 Test dates: Ma y 08*15, 2018 Concen tration Repllcate n umber Survival Average reproduction Coeffld entof Percen t reduction (mg/L NaO) (%) (offspri ng/female) var iation (%) fr om contro l (%) 1 l 3 4 s 6 7 8 9 10 Control 29 33 28 30 32 31 29 32 30 29 100 30.3 S.4 Not applicable 600 31 34 31 31 33 33 32 32 30 29 100 31.6 4. 8 -4 .3 800 34 32 30 33 32 32 29 32 31 32 100 3 1.7 4.S -4.6 1000 33 29 27 33 30 B 31 32. 32 30 100 31.0 6.S -2.3 1200 19 10 13 12 8 l2 15 12 17 7 100 12.S 30.0 58.7 1400 1 4 3 1 3 l 2 5 3 1 100 2.4 59.6 92.l Dunnet t's MSD va lu e: 2. 172 MSO: Mi nim um Significant Difference PMSD: 7.2 PMSD= Perce nt Mini mum Significant Difference PMSO ls a measure of 1esl precision . The PMSD ,s the minimum percent difference between the control and treatment tha t can be declared s1atisticallv 5ignif,ca nt in a w hole effluent toxicitv test 1 Lower PMSO bound deter mined by USEPA (10 " percentile) = !3%. 1 Upper PMSO bound de term ined by USEPA (90 " percentile) = 47%. Lowe r and upper PMSD bounds were determined from the 10t h and 90th percentile, respectively, of PMSD data from EPA's WET lnterlabor'iltorv Variablh\V St udy (USE PA, 2001a; USEPA, 2001b). The lower PMSD bound represents a practical limit to the sensit1v1tv o f the tes t method and ,snot a m inimum acceotance cri teria.
-;r USEPA. 2001a, 2001b. fin3I ~eport: lnterlaboratory Va riabllitv Study of EPA Sho11 -\.e rm Chronic and Acute W hole Elfhienl To~iclty Test Me thods, Volumes 1 and 2-Appeoclix EPA- 821-8-01*004 and EPA-821-8-01-005. US Environmental Protection A11ency, Cincinn.111, OH
Statistical Analyses Cerlodephnia Survival and ReproducUon Test-Reproduclion Start Date* 5/8/2018 Test ID* CdNaCICR Sample ID REF-Rel Tolocent End Date* 5/15/2018 Lab 10* ETS,Envir Tesung Sol Sample Type NACL-Sod1um Chlor,de Sample Date* Protocol' FWCHR-EPA-821-R-02*013 Te& Species: CD-Cenoaapnn1a dub1a Comments Conc-mglL , 2 3 4 5 6 7 8 9 10 0-Con of 29000 33000 26.000 30000 32 000 3 000 29.000 32 000 30000 29 000 600 31 .000 34 000 31 000 31 000 33 000 33000 32000 32000 30000 29000 800 3" 000 32 000 30 000 33 000 32 000 32000 29000 32000 31 000 32.000 1000 33 .000 29000 27000 33 000 30.000 33 000 31 000 32000 32.000 30 000 1200 19 000 10000 13000 12.000 8 000 12. 00 15 000 12 000 17 000 7 000 1400 1 000 4000 3000 1 000 3 .000 1 000 2000 5 000 3000 1 000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-m9/L Mean N-Mean II/lean Min Max CV % N Sum Critical Mean N -Mean D-Control 30 300 1.0000 30.300 26.000 33000 40 1 0 31 200 1 0000 600 31 600 1 0429 31 600 29 000 34 000 4 764 10 127 00 75.00 31 .200 1 0000 800 31 700 1.0462 31 700 29.000 34 000 4 474 10 128 50 75 00 3 1 200 10000 1000 31 .000 1.0231 31 000 27.000 33 000 6452 10 118.SO 75.00 31 000 0 9936
- 1200 12 500 0.4125 12.500 7 000 19 000 29 993 10 55 00 7500 12 500 04006
*1400 2400 00792 2 400 1 000 5000 59 577 10 55 00 75 00 2 400 00769 Aux Illa!)'. Tests Statistic Critical Skew Kurt Kolmogorov O Test 1n<.*ca tes normal dlstnbuuon (p > o 01) 061747 1 035 014429 1 55997 Banleirs Test indicates un!!9ual vanances !e = 8.62E-03) 15 446 15,0863 H~eothesis Test (1 -tall, 0 .05) NOEC LOEC ChV TU Steel's Many-Orie Rank Test 1000 1200 1095 45 Treatments vs o .control Linear Interpolation (2.00 Resample l Point mg/L SD 95'!. CL Skew IC05 1014 7 7.02626 1001 38 1018 62 -3 9420 IC10 1031 .57 4 98651 1015.85 1037 46 -0 7722 iC15 1048 43 5 27534 1036 35 1056 35 -0 3960 ,.o IC20 1065 3 5.75815 1053 84 1075 32 -0 0539 0.9 IC25 1082.16 6 .39113 1070.95 1094 63 0 19 3 IC40 11 216 8 83718 1118 91 t 152 13 04684 08 IC50 1166.4 10.7131 115017 1191 85 0 5230 07 0.6
~
0 05
- a. 04 ti II'. OJ 02 0 1 00 - ... - .
-01 0 500 1000 1500 Dose m9/L Dose-Response Plot 40 ~ - - - - - - - - - - - - - - - - - - - - - .
35 30
.2 25 tl j
'ti 20 E
0. ti_ 15 10 5 ._ . 0 +----""T""----,-----,.-----,-----1
~
~
Page 91 of94
Statistical Analyses E.nvJ, onm*"t* I fiiltl n!a Soklllon,. f Used for PMSD calculation only. Ceriodaehnia Survival and Reeroduction Tes-t-Reeroduction Start Date 5/8/2018 Test ID. CdNaCICR Sample ID: REF- Ref Toxicanl End Date
- 5/15/2018 Lab ID: ETS- Env,r. est,ng Sol Sample T pe NAC -Sodium ch lond Sample Da t Pro tocol* FWCHR-EPA-821 -R 013 Test Species: CD-Certodaphnia dub,a Commen ts Conc-m9/L 1 2 3 4 5 6 7 8 9 to D-Conlrol 29 000 33.000 28.000 30 000 32 000 31 000 29.000 32 000 30.000 29.000 600 31 .000 34 .000 31 .000 31 000 33.000 33 .000 32.000 32.000 30 000 29.000 800 34 000 32.000 30 000 33.000 32 .000 32.000 29.000 32 .000 31 000 2.000 1000 33 000 29 000 27.000 33.000 30.000 33.000 31 .000 32.000 32.000 30.000 1200 19.000 10 000 13 000 12.000 8.000 12 000 15 000 12.000 17.000 7.000 1400 1.000 4 000 3.00 0 1.000 3.000 1 000 2 000 5.000 3 000 1.000 Tra nsform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSO D Control 30.300 1 0000 30 300 28 000 33 000 5.401 10 600 3 1.600 1.0429 31 600 29 000 34 .000 4 764 10 - 1.369 2.287 2.172 800 31.700 1.0462 31 .700 29.000 34 .000 4 474 10 -1.474 2.287 2 172 1000 31 .000 1.0231 31 .000 27.000 33 000 6.452 10 -0.737 2.287 2. 172
' 1200 12.500 0.4125 12.500 7 000 19 000 29.993 10 18.744 2.287 2. 172
'1400 2.400 0 .0792 2.400 1 000 5 000 59 577 10 29379 2.287 2.172 Auxilla!l'. Tests Stati tic Critical Skew Kun Kolmogorov D Te t Indicates normal distribution (p ::. 0.01) 0.61747 1.035 0.14429 1.55997 Bartlett's Test tnd1cates unegu.al variances !e = 8 62E-032 15.446 15..0863 H:ieothasls Tes t j1-tall, 0.05) NOEC LOEC ChV TU MSOu MSDe MSB MSE F-Prob dt Dunnett's Test 1000 1200 1095.45 2 171 55 0 07167 1602.35 4 50926 5.2E-40 5, 54 Treatments vs D-Contr ol Page 92 of94
l 0 C Pa.,e 5 of6 Species: Ceriodaplmia dubia CdNa CIC R #: 215 D auy
*1 Cl.zenus 1y:
l Da (Analyst identified for each da ,, pcrfonned pH. 0 .0. and cond uctivi ty mcasurcmcnts only.) 0 I 2 A n alys t rl.r H5 H.5 tlv k..J rlS' Co n c e ntration Parameter pH (S.U.) DO (mg/L) Co ntlu clivl ty
+ .e,4
--:,.. =1 7/.9'.5 1-*B
- /.9(3
'1 * (:J .,.~
Q;.o , e.o l
,i."1-8 . oo f*B (iunhos/cm) 3 / t- 3 10 52"'
CONTROL *Allrn linity (mg CnCOi/L) S'\ SC\ ~'-.t
" I li1rdn css g(o (me. CaC01/L) ~lo v* "" !
*Tempcrn turc (0 C) '"l.-\ . i- ""\J,
- 0 "'\.'°( *"\ ~.\ "'l.'\.& ""\.S. '
pH (S.U.) R.,v, ":l..,,~ ":/-. Cf I '+.~5 ~ l )lA -:/.9b DO (mg/L) 7,. :, -i,5 ":(. 'I] ~.+ ~.ll 7-e 600 mg NaC I/ L Co11 du c1h*ity (µmhos/cm) IL/:JO 1390 l'f0°
" T c mp cr11turc (oC) ""\.."-\-'\ 1.L\ . f 1..-l . &- ""\>L~ "'l.i.\..'\ "'\.~ . 0 p H (S.U.) R -oo ':l-9Z ?.93 'l-.t:\v 0.04 °'"::/-'7~
800 mo NaCI/L 00 (mg/L) Co nductivity (µmhos/cm)
*Tcmpc rntu i-c (°C)
+8
/(j()()
""\...\*.So
--=,. e, "1.'{
- 8
-:,.. er l f3 / 0 iJ.l . .f'
'+.e
-u-\.i
,~o t:\I 1.'t, '\
71-e
-is .,_
pH (S.U.) 7/.qq "1. 413 -:,. 9 3 '\~S' S.04 ? -9~ DO (mg/L) ~ '1 -=f. g ?..<t '+~ '+,Ii" ":/-EE I 000 mg NaCI/L C ontluclivfl * = (µ mhos/cm) Z l 'Bo "2 ltoO a.Hoo
*T emperature (oC) "'l..S .o ""\.S . o ....t.\." lS.I "'l...'{,'l ,s..,_
pH (S .U.) "7/. 'J"t 1-'12 ":{. 93 'l-!l4 0~ ":f..9J.f DO (mg/L) 8. o 8-o -=,."' '},e ~~ -=,.. 7 1200 mg N aCl/ Co ntlu ctivity (urn hos/cm) 2"-1&/o zsoo asaJ
*T emp1:rnturc (0 C) '\.'-l.c:.; "bl,."\ "'\.4,, "L*I. '\ l '\."\ b .O pH (S.U.) "'1-. "16 -=,.'12.. -:,. "!:1 l'}.~..cr e.o3 -=,.. 9 '1 DO (mg/L) f?, L ~o ff..;, ~-q ~~ -=,.=,
1400 mg NaCI/ L Co nductivity (µmllos/cm) 2 9 ()() 2 B "7o ~eqo
*Tem perature (0 C) "1.'*l.'l "1..-l ,'\ i.'-l. &- ""\.S. 0 '1.-t.1 '15 -D Initial Final Initial Fin a l lni tial F in a l
- icmpcraturcs performed al the time of test initiat ion , renewal or 1cm1mation by the analyst iden tified in the Daily Renewal lnfonnation table located on Page I. Alkalinity and hardness perfom1cd by the analyst identified 0 11 the ben h sheet specific for each analysis and transcribed to this bench sheet by: 6, Page 93 of94 SOP AT14 - Exhibit ATl4 . 1, re ision 11 14
Page 6 of6 pecies: Ceriodapl111ia dubia CdNaCICR #: 215 Day (Analyst identified for each day, performed pH. D.O. nnd conductivity measurements onlv.l I Concentration Para meter Analyst Hf 3 14::.1.., P..c.c... 4 J....
- 5 IA./ J._)
6
/4.S I 11H (S.U.)
DO (mg/L) Conductivity
+.91 t *'1
,.,,gs
'!,o "J. .~s-f-,lc, e,,o4 1..6 0 .\18
".J..'}
e.ol
~-q e,.oo
'la 1.f!~
-:Z.q (umhos/cm) 305 ..? "'i .. &>0 !>oo l
I CONTROL *Alk:ilinily (m~C:iCO.i/L)
- Jfardn l' (mit CaCOJ/L)
.. Temper:iture
(°C) O'"t..'" "'
-\.g '"'!. s .\
r
"'a
&'-\
1.-\* ~ "V>> . 0
~" "
'1.A .1 "'\S,. 0
~..,a
~
,._4.,
-i.s . "L pH (S.U.) P..oo -i...&5 t-.q\ 6.0\ ~ .Ol ~.q"} a .02. -:J.~
I DO (mg,'!..) Contl uctivity 7 -'":/- '3-0 1-,~ 'l.e '+~ 'l, ~ ~~ -:i. Cf 600 mg NaCI/L 1300 (11mhos/c111) l~~o t:Ffo l~ b
- T em pernture
'\.'-\.. '\ "l..-\ . ~ "l."\. g , A. i "'lS" , 1.. i..S.O "L*l .'\
I (oC) pH (S.U.) ~.ol 1-.i i 1-.q 1
'1..'-\ *"
A .b O 8 .0 1 'i-~:\- e.o3 1.e::, DO (mg/L) 7/.B ~.o 1-.<t" '1,8 "1--8 ~ -q t:\A "':f.<1.f I 800 mg NnCI/L Contl uc livily (11mhos/cml
*Temperature
/ BE,o LJ-\.'\ '-~ * ~
1~<>0 t.**V\ 1.-\.~ llvo 1.~-1 u.o 1euo tS -0 t.S.o ("C) I p H (S.U.) DO (mg/L) 8 .a1 7.6
+,iq
~~
+.'l'l
+-'l q.qq
'1-4 P,.L)3
' ~fl
').~"}
~A (3,0,{
f> ,O
'"=1-8+-
-:/. C, 1000 mg NaCI/L Conducth*ity (u 111hoslc111)
"Temper :iture 2210
'"l..1.\.-cf 1.A.'\
;1.1 <, 0 c,\ .g '"l..<;. \
~130
"\S .D
"~c i,S.Q ""\.l.\ ,'i
(*C) L'1*1 pH (S.U.) '8.00 1-.q\ t.~ ~kl. e,.o~ '+.'\"}- ~.o4 -=,. rn-00 (mg/L) e .o +J:i 1-A /.}(J ~ e,,o 8 '° fg. I J Conduclivity 12 0 mg Na 1/L
<umhos/cml -z..!,--eo ~510 ,, ~SOo ~$lo
*Tcmpernture c*C)
'\..'-\.. g- '1..S. \ ""t'-\ . i '\.'-\ . f '\_~ ., \$ . \ -i.s..o
'1.S- \
J pH (S.U.) 80 1 '5' ,00 +.'i!1- ').(\<,, (!) l'f2. I). 41A d.04 ':/-8'1 DO (mg/L) &-o "'1-Z 'if.o rJ.0 ~A e.o e> JJ R. t Condu cti vity 1400 mg NaCI/L (umhos/cm) 2 9 ?"0 ~85o '-wo ~q"'n '
*Temper:iture 1-L\.~
("C) "l.~ .'\ '1.'-\. g , A. ( 1.~ -'\ ~ .\ 1.~.o 1..~.f Initial Final Initial Final Initial Final Initial Final
.,.e111pcrmures performed ot thc time oftest ini1ia1ion, renewal or termination by the analyst ident ified in the Dail Renewal lnformntion !oble located on Page I. Alkalinity and ha dncss perfonned by the analyst identified on the bench sheet specific for each anal sis and trnnscribed 10 this bench sheet by: _ .,....._ .
SOP AT l4 - Exhibit ATl4 . I, revision 11 14}}