Text

Submittal of Corrected May 2016 Toxicity Report
	ML16256A778
Person / Time
Site:	Sequoyah
Issue date:	09/07/2016
From:	Schwartz C; Tennessee Valley Authority
To:	Hall A; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources
References
	TN0026450
	Download: ML16256A778 (88)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 September 7, 2016 Ms. Angela Hall Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243

Dear Ms. Hall:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - May 2016 Toxicity.Report - Corrected ) Enclosed is a corrected copy of the May 2016 Toxicity report. The toxicity report previously submitted with the June 2016 DMR may have had missing pages. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.. I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Sincerely, Enclosures cc (Enclosures): Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 Mccallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION I EXECUTIVE

SUMMARY

Report Date: June 16, 2016

1. Facility I Discharger: Seguoyah Nuclear Plant I TV A

2. County I State:. Hamilton I Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River CTRM 483.6)

7. lQIO: 3,491

8. Outfall Tested: 101

9. Dates Sampled: May 15-20, 2016

10. Average Flow on Days Sampled (MGD): 1794, 1797, 1789

11. Pertinent Site Conditions:* Production I operation data will be provided upon request.

12. Test Dates: May 17 -24. 2016

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested(%):

Pimephales promelas: UV treated Outfall 101: 10.7, 21.4. 42.8, 85.6, 100 UV treated Intake: 100 Ceriodaphnia dufoa: Non-treated Outfall 101: 10.7, 21.4. 42.8, 85.6. 100 Non-treated Intake: 100

16. Permit Limit Endpoint(%): Outfa.11101: ICJs = 42.8%

17. Test Results: Outfall 101: Pimephales promelas: IC?5 > 100%

Ceriodaplinia dubia: ICJ5 > 100% Page 1of87

18. Facility

Contact:

Millicent Garland Phone#: (423) 843-6714 ~9. Consulting I Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone#:. (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone#: (256) 386-2787

22. Notes: Exposures to samples collected May 15 - 20, 2016 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC2s values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Exposure of minnows to intake samples reduced minnow growth significantly from the control.

Page 2of87

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY) Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (aC) (mg/L) Last Used By 101 05-15-16 0705 to 05-16-16 1250 3 t <0.10 05-17-16 1333 05-16-16 0605

1. ' 1.5 05-18-16 1234 Intake 05-15-16 0720 to 05-16-16 1250 2.4

<0.10 05-17-16 1333 05-16-16 0620 05-18-16 1234 101 05-17-16 0700 to 05-18~16 1200 1.0, 1.2t <0.10 05-19-16 1233 05-18-16 0600 05-20-16 1240 Intake 05-i7-16 0715 to 05-18-16 1200 0.4 <0.10 05-19-16 1233 05-18-16 0615 05-20-16 1240 05-19-1.6 0650 to 05-21-16 1237 101 05-20-16 1225 2.0, l.Ot <0.10 05-22-16 1250 05-20-16 0550 05-23-16 1305 05-19-16 0705 to 05-2}-16 1237

Intake 05-20-16 0605 05-20'-16 1225 1.8

<0.10 05-22-16 1250 05-23-16 1305

TRC =Total Residual Chlonne tsamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were wanned to test temperature (25.0 +/- l.0°C) iri. a warm water bath.

Aliquots ofOutfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. Page 3of87

Pimephales promelas Test Organisms:

1. Source:

In-house Cultures

2. Age:

< 24-hours old Test Method Summary:

1. Test Conditions:

Static. Renewal

2. Test Duration:

7 days

3. Control I Dilution Water: Moderately Hard Svnthetic

4. Number of Replicates:

1:

5. Organisms per Replicate: lQ

6. Test Initiation: (Date/Time): 05-17-16 1333 ET

7. Test Termination: (Date/Time): 05-24-16 1238 ET

8. Test Temperature: Outfall 101:

Mean= 24.7°C (24.2 -25.0°C)

9. Physical I Chemical Ceriodaphnia dubia In-house Cultures

< 24-hours old Static. Renewal Until at least 60% of control females have 3 broods Moderately Hard Svnthetic l 05-17-16 0827ET 05-24-16 0830 ET* Mean= 24.8°C (24.2 -25.2°C) Measurements: Alkalinity. hardness, total residual chlorine. and conductivity were measrired at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre~ and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Page4 of87 Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKin:Ilevville, CA).

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 17 - 24, 2016 using effluent from Outfall 101. Test Percent Surviving' Solutions (time interval used-days) (%Effluent) 1 2 3 4 5 6 7

Control, 100 100 100 100 100 100 100 UV-treated 10.7%

100 100 100 \\ 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Control, 100 100 100 100 100 100 100 Non-treated Test Solutions Mean Dry Weight (mg)

(% Effluent) (replicate number) 1 2 3 4 Mean

Control, 0.470 0.421 0.485 0.552 0.482 UV-treated 10.7%

0.546 0.585 0.593 0.507 0.558 21.4% 0.505 0.470 0.614 0.563 0.538 42.8% 0.509 0.516 0.514 0.564 0.526 85.6% 0.439 0.455 0.478 0.452 0.456 100.0% 0.453. 0.482 0.428 0.491 0.464 Intake 0.378 0.460 0.405 0.406 0.412

Control, 0.573 0.555 0.565 0.628 0.580 Non-treated IC2s Value: > 100%

Calculated TU Estimates: < 1.0 TUc* Permit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/ IC2s Page 5 of87

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 17 - 24, 2016 using effluent from Outfall IOI. Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 10.7% 100 100 100 100 .100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 .100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 *Mean Control 33 29 30 29 27 29 ~7 28 29 29 29.0 10.7% 29 32 34 29 28 31 29 30 29 32 30.3 21.4% 31 30 31 35 30 29 27 32 32 30 30.7 42.8% 32 34 32 32 33 33 29 30 32 35 32.2 85.6% 34 33 34 32 36 31 35 32 29 31 32.7 100.0% 34 35 36 33 37 35 34. 37 33 33 34.7 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Pennit Limit: 42.8% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC2s Page 6 of87

TOXICITY. TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type I Duration) Conducted May 17 - 24, 2016 usin$ water from Intake Percent Surviving Test (time interval used ~ days) Solutions 1 2 3 4 5 6 7 (%Effluent) Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) 1 2 3 4 5 6 7 8 9 10 Mean Control 27 26 28 29 31 30 31 29 30 32 29.3 Intake 34 35 32 28 31 29 30 29 32 32 31.2 IC2s Value: > 100% Calculated TU Estimates: < 1.0 TUc* Pennit Limit: N/ A. Permit Limit: N/ A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/ IC2s REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (lCzs) Pimephales promelas May 17 -24, 2016 1300 7-days KCl 0.64 g/L Ceriodaphnia dubia May 10- 17, 2016 0940 7-days NaCl 1.06 g/L Page 7of87

Pl-IYSICAL/CHF:M ICAL

SUMMARY

Water Chemislly Mean Values and Ranh>es for UV-lrca!cd Pimeplwles pm111elas and Non-treated Ceriodaplmia dubia. Sequoyah Nuclear Plant (SQN), Ellluenl Outfall I OJ and lnlake pcrl01111cd May 17-24, 2016. Test Sample ID Temncmiurc ("CJ Dissolved OXY!!en (m!!IL) Initial Final Initial Final

Cnntrol, 24.7

.24.5 7.8 7.8 Nun-lrcalcd 24.5 - 24.8 24.3 - 24.6 7.7 - 7.8 7.7 - 7.9

Cnnlrnl, 24.8 24.5 7.8 7.8 llV-ln*nlc.*d 24.7 - 24.8 24.2 - 24.6 7.7 - 7.9 7.8 -

8.0 10.nr.1 24.8 24.5 7.8 7.8 24.7 - 24.9 24.3 - 24.7 7.7 - 7.9 7.8 - 7.9 24.8 24.5 7.9 7.9 1: 21.4"/.1 ~ 24.7 - 24.9 24.3 - 24.7 7.8 - 7.9 7.8 - 7.9 ~

24.8 24.5 7.9 7.8 42.8'X1

..::: e-24.7 - 24.9 24.3 - 24.7 7.8 - 8.0 7.7 - 7.9 ~ 85.6'Yo 24.8 24.6 7.9 7.9 24.7 - 24.9 24.3 - 24.8 7.8 - 8.0 7.8 - 8.0 100% 24.9 24.5 7.9 7.9 24.8 - 25.0 24.2 - 24.7 7.8 - 8.0 7.7 - 8.0 Intake 24.8 24.5 7.9 7.9 24.7 - 25.0 24.3 - 24.8 7.8 - -8.0 7.8 - 8.1

Cnnlrol, 24.8 24.8 7.8 7.8 Non-I rented 24.7 - 24.9 24.4 - 25.2 7.7 -

7.9 7.7 - 7.8 I0.7% 24.8 24.8 7.8 7.8 24.7 - 25.0 24.6 - 25.0 7.7 - 7.8 7.7 -- 7.9 24.8 24.7 7.8 7.9 oC) 21.4'Yc1 .§ .24. 7 - 25.0 24.6 - 24.8 7.7. - 8.0 7.8 - 7.9 24.9 24.7 ' 7.9 - 7.9 42.8°/.1 §< 24.7 - 25.0 24.2 - 25.0 7.8 - 8.0 7.7 - 7.9 24.9 24.7 7.9 7.9

~

85.6% d 24.8 - 25.0 24.4 - 25.0 7.8 - 8.0 7.7 - 8.0 !00% 24.9 24.7 7.9 7.9 24.8 - 25.0 24.4 - 24.9 7.9 - 8.1 7.7 - 8.0 Intake 24.9 24.8 7.9 7.9 24.8 - 25.0 24.4 - 25.l 7.8 - 8.1 7.8 - 8.0

Nole: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

Overnll tempernture ( 0C) Pimeplwles pro111e/as Ceriodaplmia tlubia Avemgc 24.7 24.8 Minimum Maximum 24.2 25.0 24.2 25.2 pH (S.U.) Conductance Alkalinity Hanlncss

Total Residual Initial Final (111nhos/c111) (mg/L CnC0.1) (mg/L CaC03)

Chlo1ine (mg/L) 7.48 7.20 313 61 87 7.30 - 7.65 7.00 - 7.48 299 - 322 61 - 62 86 - 88 7.45 7.23 . 307 62 88 7.22 - 7.64 6.97 - 7.63 297 - 321 61 - 63 88 - 88 7.44 7.22 296 7.23 - 7.65 6.95 - 7.58 287 - 307 7.43 7.22 282 7.23 - 7.65 6.99 - 7.61 273 - 290 7.42 7.20 255 7.23 - 7.64 6.95 - 7.56 251 - 261 7.42 7.17 205 7.22 - 7.64 6.90 - 7.52 201 - 208 7.41 7.23 187 62 71 <0.10 7.20 - 7.63 6.98 - 7.53 183 - 192 60 - 64 68 72 <0.10 - <0.10 7.41 7.25 199 65 73 <0.10 7.18 - 7.66 6.97 - 7.63 196 - 205 64 - 68 68 - 80 <0.10 - <0.10 7.49 7.35 313 61 87 7.30 - 7.65 7.13 - 7.56 299 - 322 61 62 86 - 88 7.43 7.35 289 7.22 - 7.63 7.17 - 7.59 261 - 312 7.44 7.33. 282 7.24 - 7.64 7.08 - 7.59 274 - 291 7.43 7.33 254 7.22 - 7.64 7.11 - 7.59 247 - 261 7.42 7.32 - 204 7.20 - 7.63 7.05 - 7.59 201 - 210 7.40 7.31 186 65 75 <0.10 7.17 - 7.63 7.06 - 7.59 182 - 191 64 66 72 76 <0.10 - <0.10 7.42 7.33 198 71 73 <0.10 7.21 - 7.62 7.11 - 7.58 188 - 204 68 - 73 72 - 76 <0.10 - <0.10

SUMMARY

I CONCLUSIONS Exposures to samples collected May 15 -20, 2016 from Outfall IOI resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Exposure of minnows to intake samples reduced minnow growth significantly from the control. Page 9of87

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02~013 (October 2002) using four replicates, each containing ten test iorganisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-82l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, 1each containing 15-mL oftest solution.

DEVIATIONS I MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas Samples used in the fathead minnow test were exposed to UV light for two minutes prior to introduction of test organisms. UV treatment is used to control interference of fish pathogens. This treatment n;iethod was approved on November 23, 2015.by the State of Tennessee in a letter from Jessica Murphy to Terry Cheek, Senior Manager of TV A Water Permits, Compliance, and Monitoring. *

2. Cerfodaphnia dubia None DEVIATIONS I MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS*

I.. Pimephales promelas None

2. Ceriodaphnia dubia None Page 10of87

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before t;ixpiration dates (where applicable).

I

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments i~ recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2000.

4. Dissolved oxygen was measured by SM 4500-0 G-2001.

5. The pH was measured by SM 4500-H+ B-2000.

6. Conductance was measured by SM 2510 B-1997.

7. Alkalinity was measured by SM 2320 B-1997.

8. Total hardness was measured by SM 2340 C-1997.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. . REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCL

2.

Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. Page 11of87

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEP A. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Omanisms. EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater. 22nd Edition, 2012.

4. Quality Assurance Program: Standard Ooerating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 12of87

Page 13of87 Sequoyah Nuclear Plant Biomonitoring May 17 -24, 2016 AppendixB Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 20, 2016 Date $~1um. Towerbrom * *pct:;::222 PCL-401 HYJ>Ci9bl()rit~ mg!L

fug/r; ***

mo/L TRC

.Ph_o.:;:rih_

.*.... _a_t_-~-**.. *.. Copolymer . *.:. JllT~:_.. _.R&fIJ_._*c*_*-.. --*._. "'r- -( '. ~~ *' ~ :-,~_- : 02/06/2005 0210712005 02/08/2005 02/09/2005 02/10/2005 02/11/2005 06/05/2005 06/06/2005 06/07/2005 06/08/2005. _, 06/09/2005 0611012005 07/17/2005 07118/2005 07119/2005 07/20/2005 07/21/2005 07/22/2005 10/30/2005 10/31/2005 11/01/2005

~..

11/0212005 11/03/2005 11/04/2005 7 11114/2005 11/15/2005 I 1/16/2005 11/17/2005 11/18/2005 11/19/2005 'Page 14 of87 <0.0042 <0.0116 <0.0080 0.0199 <0.0042 0.0155 0.0063 0.0043 0.0103 0.0295 0.0129 0.0184 0.0109 0.0150 0.0163 0.0209 0.0242 0.0238 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 0.0274 0.0256 0.0234 0.0231 0.0200 0.0116 . 6~02~.,. 0,026**:; i"lil~~ 0.010 0.010 0.010 0.010 0.010 0.010 0.009 0:009 0.009 0.009 0.009 0.018 .*-~ --,:.:.~.. Nalco 73551 mg/L EOIPO 0.007 0.007 0.014 0.014 Ji:l5QM

niWL

- Quat 0.037 0.037 0.037 0.036 '0.036 . 0.036 O.Q35 0;036 0:036 0,035

/ Date 11112/2006. 11/13/2006 11114/2006 11115/2006 11/16/2006 11/17/2006 *- 11/26/2006.. 1112712006. 11128/2006 11/29/2006 11/30/2006 12/0112006. 05/28/07 05/29/07 05130107 05/31/07 06/01/07 06/02/07 12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07 04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04118/08 10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08 Table B-1. Sequoyah Nuclear Plant Outfall IOI Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - May 20, ~016 0.0260 0.0151 0.0172 0.0154 0.0086

l:, _._,.

. ' - ~.

PCL-401 mg/L Copolymer -*.~.

- r.,

~-- - -.-. - ' ~..:*. - 0.017 1 0.018 Page 15 of 87 -

\\0.037 t~o:o~1 -

'.,_6,037 . :;Q.037 -. ~- '='_'.{L036 '::.;9p'~6. >,.*0.0.:'16

.yo;o36

0;041 0~041 0,041

. 0.041 MSW 101 mg/L Phos hate 0.015 0.015 O.Dl5 O.Dl5 O.Dl5 0.015 0.030 0.030 0.030

Date 02/08/09 02109109 02110109 02111109 02/12/09 02/13/09 05110109 05111109 05/12/09 05113109 05/14/09 05/15/09 11115/09 11116/09 11/17/09 11/18/09 11119/09 11/20/09 05109110 05110110 05111110 05112110 05/13110 05/14110 Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 20, 2016 0.0197 0.0237 0.0104 '0.0155 0.0106 0.0129 ' ~ 0.0415 O.Oo53 0.0049 <0.0141 <0.0160 0.025., 0.0152 0.0255 Page 16 of87 H-150M

MSW.

mg/L

101.

Quat -mg/L 0.0446 0.0396 0.0396 0.0397 ~Jiosph~te

r.

~.:. -

Table B-1. Sequoyah Nuclear Plant Outfall IOI Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, Date 10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10 05/01/2011 0510212011 05/03/2011 05/04/2011 05/05/2011 05/06/2011 c 11/06/2011,_ - 11/07/2011 - - 11/08/2011 11/09/2011. 11110/2011 11111/2011.. 05/06/2012 >"'":.!.';/' 05/07/2012. 05/08/2012 -... - 05/09/2012 ** 05/10/2012 \\' 05/11/2012 - 08/12/2012 08/13/2012 08/14/2012 08/15/2012. 08/16/2012 ' 08/17/2012 ! -., \\ 05/12/20i3 05/13/2013 ~-. 05/14/2013 ' 05/15/2013 05/16/2013 05/17/2013 09/15/2013 09/16/2013 09/17/2013 09118/2013 09/19/2013 09/20/2013 0.0256 0.0209 0.0279 0.0076 0.0446 0.0099 0.0091 0.0096 0.0229 0.0063 0.0072 0.0107 0.0217 0.0172 0.0173 Page 17 of87 February 6, 2005 -May 20, 2016 Nalco 73551 mg/L EOIPO 0.028 0.039 0.039 0.036 0.036 .'.'Specfuis; H-150M 'MSW Floguard ,;¢$,1J30Q( mg/L i )01-MS6236 !1~.* Quat ',~. Ph:'~ O~D3T'-;

-BY0_)1-. -*

~*: ~...: '~.

~* *:*"* .? :

O:Q~79 0.0379 0.0379 ,:,~'. 0.029 0.029 0.029 0.029 0.029 0.032 0.064 0.064 0.064 0.032 0.032 0.03 0.03 0.03 0.03 0.03 0.03

Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Mici-obiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 -May 20, 2016

S9.4Nw': Towerbro

H:W~'

mg!L Date clilorite TRC .(${~.~ 05/04/2014 05/05/2014' 0510612014 ' 05/07/2014 05/08/2014 05/09/2014 09/07/2014 09/08/2014 09/09/2014 09/10/2014 *. 09/11/2014.:* ~-*.-.... 09112/2014 08/09/2015 08/10/2015 08/1112015 08/12/2015 08/13/2015 08114/2015 10118/2015 10/19/2015 10/20/2015 10/2112015.. -**-

~,'*

10/22/2015 10/23/2015 ' 05/15/2016 ' 05/16/2016 05/17/2016 05/18/2016 05/19/2016 ' ' 05/20/2016 0.0118 0.0112 0.0096 0.0164 0.0235 0.0110 0.0070 0.0074 0.0130 0.0174 0.015p 0.0209 0.0210 0.036'1 0.0254 0.0261 Page 18 of87 . / 0.03 0.03 0.03

l.

Page 19of87 Sequoyah Nuclear Plant Biomonitoiing May 17 - 24, 2016 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page~l~of~l~ 'l!jent TV A Environmental Testing So~ution, Inc. Delivered By (Circle One): FedEx UPS Bus -Client '@oject Name: Sequoyah NP Toxicity 351 Depot Street. ~

Sonic... V-eli'11e.ry

@:>.Number: NIA Asheville, NC Other (specify): ~ility Sampled: Sequoyah NP . 28801 General Comments* 1ii(.)K.~awrp!r:5 f1r(),,,., /{)/.Ja,..jJlc-l"t? 06?.'i"O NPDES Number: TN0026450 Phone: .. 828-350~9364 I /:,,k ~7/f"f IJ-"n..Z-,ralc~ 5~?kre {)7i/ 7 C II d B /11~r~"-' tJ. /r?oNt' f >i<l~o.f" Fax: 828-350~9368 Jf /!)/ 8ac4 ~,,Pl~ vJ!l~../A'):',?ee/.z:b 6'v/~,.,.,-e.tf< o ecte~ ~ ~.-) /

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(/ v I I P"OJ e,U-~, lLl90 Container Field Identification I Number& Flow Rain Event? S11rt1ple Description Grab/Comp. Collection Daterrime Volume (MOD) Laboratory Use Collected (Mark as Appropriate) ~y*, .. :~* *;:2.<*,* -,;j:_*'. '..:\\.-.:*::~ ;_'. :'.:,;::, '.'>~i.. J : .. :) ~: Siert End ~,]~::-~~~;::~;{ i~iJ 0:~'.~~{~:{r' Yes If Yes, No Trace ETS Log Arrivnl.Tcmp. By Time Appear-Inches Number c*c> (.1 once S-15'"--/(,, 5'-/t,-11;. '/ \\\\.105\\~.0) \\$\\\\.C:,"C.... \\t.40 -1. SQN-101-TOX Comp 2(2.5gal) 1'11'3. si& ~- 1J10~ &.Jr /)IPOS° $JT SQN-INT-TOX Comp ~15'*/{p .)-/(, -/{;, 1(2.S gal) fl/II. 1 l~c.Gll? Dt.- -z.A *t. t \\-t:,u /.. ~7:JO G'JT' {) {p ;J.() fjr ~ Sample Custody - Fill Jn From Top Down f: C".S"TI>t\\-l 5eAl5 \\r.JTK,\\, ~~ ...ec. ~l'l\\Je.r. Relinquished By (Signature): Date/Time Received By (Signature): LK) 6-to~ COt-)b.lTl°l), ffc_ A~~ 1"1 Al..L at~ 1me SH-\\~,,)( d //Id -r\\J t\\

)-/to-1~lo?j(o'f:. A A1 ~

5Hl\\(. bet..\\~ ~'\\ 0.5-- /l-/f; / 0 ~:/6' ET r C../.7\\. .? !hi_ Sa~\\L. ( ~~Iv.-- A~ Jb .S':. t 6.. / J, /;:J;$0 < ~ trs os-11.o ~11.:> * 'heii~ ~ '1.Sa ET f .v I Instructions: Clients should fill in all areas except those in the *'Laboratory Use" block. Biomonitoring samples arc preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore. please collect and ship in such a way that !he labornlory will receive the st1mples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not an*ivc until the following Monday.

Whole Effluent Sample Receipt Log

sample temperature performed using Sample Receiving Thermometer: SN 130580085 Date Time Received,

Received

Sample Project Sample Sample name and description,_

State Comments Received Received by from Temp. (°C) number number 05*16*16 1250 J. Sumner TVA Courier 1.3/1.5 11480 160516.01 TVA - Sequoyah Nuclear Plant-101 TN 05*16*16 1250 J. Sumner TVA Courier 2.4 11480 160516.02 TV A* Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

~ent: TVA fi,ject Name: Sequoyah NP Toxicity ~*Number: N/A ~ility Sampled: Sequoyah NP NPDES Number: TN0026450 v v I I BIOMONITORING CHAIN OF CUSTODY RECORD Environmental Testing Solution, Inc. 3 51 Depot Street. Phone: Fax: Asheville, NC 28801 828-350-9364 828-350-9368 Container Delivered By (Circle One): FedEx UPS Bus Page_l_of_l_ Client Field ldcntifrdtion I Sample Description Grab/Comp. Collection Dateffime Number&' Flow Volume (MGD) Rain Event? (Mark as Appropriate) p~ e,ct--Y: I l~ 80 Laboratory Use SQN-101-TOX Comp SQN-INT-TOX Comp }elinquished By ~gnature): Stan S-1'7*/(;p O'J()D~f,1 5,1rj..J{p IJ'1 /"'S' t"-'r c' Collected End 2(2.Sgal) ()~(){) f'61' 1(2.5 gal) t>(D t 'Se~r If Yes. Inches Sample Custody-.Fill In From Top Down Date/Time Received By (Signature): ( .l) v No Trace "'f. o.ol

f.

0.01 ETS Log Arrival Temp. By Numbor C°C> / 0S.*lcr*1" \\'2.00 ET" Time <c..T Appear-ance lnstrnctions: Clients should fill in all areas except those in the *'Laboratory Use block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the lime of collection. Therefore. please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Date Time Received Received Received Received by from 05-18-16 1000 K.Keenan Fed* Ex 05-18-16 1000 K.Keenan Fed* Ex 05-18*16 1000 K.Keenan Fed. Ex 05*18*16 1000 K.Keenan Fed. Ex 05*18*16 12.00 J. Sumner TVA Courier 05*18~16 1200 J.sumntir TVA Courier 05-18-16 1451 J. Sumner TVACoui'ler OHS-16 1451 J. Sumner TVA Courier 05*18-16 1451 J. Sumner TVA Courier 05-18-16 1451 J. Sunnier TVA Courier r* . Whole Effluent Sample Receipt.log Page_kg

sample temperature performed using Sample Rccel*lng Thermometer: SN 130580085
Sample Project Sample Sample name and description State Comments Temp. (°C}

number number 0.7 11483 160518.Ol Washington WWTP NC 0.4 11484 160518 ;02 TVA - Allen Fossil Plant - Outfall 001 TN 0.3 11484 160518.03 TVA-Allen Fossil Plant-Intake TN 0.3 11484 160518.04 TVA -Allen FossilPlant-Upstream TN 1.0/1.2 11480. 160518.05 TVA-Sequoyah Nuclear Plant* 101 TN 0.4 . 11480

. 160518'.0(i TVA ~ Seauovah Ni.iclea.i' Plant - Intake.

TN 1.3 11485 160518,07 TVA. Shawnee NP - Outfall 001 AA KY 1.7 11485 160518.. 08 TVA* Shawnee NP - Outfall Intake AA KY 2.2 11485 160518.09' TVA. Shawnee NP. Outfall 001 AP KY 2.0 11485 160518.10 TVA* Shawnee NP-Outfall Intake AP KY SOP G4 - Exhibit G4.2, revision 01-03-12

BIOMONITORING CHAIN OF CUSTODY RECORD Page*_l_ of _1_ Gijent: TVA Environmental Testing Solution, Inc. Delivered By*(Circle One): fi>ject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P~. Number: N/A Asheville, NC Other (specify): Son]e_ De\\ivef( ClO 28801 General Comments: Ftltillty Sampled: Sequoyah NP . *' ~~~/,ps./;({)- /t>I' ~p1ere.!J1P?5' NPDES Number: TN0026450 .Phone: . 828-350-93 64 TV&>,,e ~?k..s /;o;-, ~I& .sa-_?le.,.._.a ~/S' r, jf- /OI o~a..e-p ~tfe ~$ 5),~p/ -60 Collected By: ~11Uc5 t>. J't'ft:JDre, r:i-n~.~~ Fax: 828-350-9368 6?Y1°reJ,..-e,rl;l'"a./ /~$_/,._J1 $1).u.~n;H -~--VP?- 'k~*;) ~ fJ1~J.P"h/...J~ µei'e 6,/~,l-kfil--ti.1t.;<<,~ {/ v . \\ .. Container. p rU)) ~ 1\\..ieo Field Identification'/ Grab/Comp. Number& Flow

Rain Event?

Sample Description Collection Datemme Volume (MGD) Laboratol;' Use Collected (Mark as Appropriate) F.t;t);\\',: ~:.:.:~; :\\~M~it{~~ ~t~~:.: ~;;:~Jt~~; S1art End . 1=~i;~;~;~~~~ f~l4~~:.Yes If Yes, No Trace ETSI.og Arrival Temp. By Time Appear-Inches Number f"Cl ance S-19,/a. s;Zo~lh x \\lQOS?b.o\\ -z.o, \\.0 't -~ "\\'il-'-, }{- SQN-101-TOX Comp C)IPsO ~ os-s-o ~-* 2(2.Sgal) 1'7gq, 1'11# 5-/11-1~ s--w-1~ x ~~(}Sll)4 t-~*c... ~ ~"'s

SQN*INT-TOX Comp

. P /,[)s a 1(2.5 gal)

J,t/,

\\ C)?&J~e-Sample Custody - Fill In From Top Down ~ tu';)T"l>b "/. .~AIC /~CT SAHiPs..e~ Relinquished By (Signature): Date/Time Received By (Signature): ~\\'\\fQ.b 1.,) ~ ~~\\"TIOt..i, -:rn..t ~~ Oat~ 1ine,w Ai'-L r.~~Wi Ii Id ~4 \\\\/~ r;~zo~/b-Jo f'/0 E ~ff If U'/,,~ Sot-1\\t. 0.:s~ Q 04.J /,(, / tJw. ( 8 'i:.' ~ l::>a..\\...JU,.-.\\ ~~ f) "-,ff / ~~\\(,. ~ o:;,.... -~ o ~l'/t:l.*°'ilJ ~ ~\\,. l\\v,../ ~ os.-to "'"' '1. 7.S E" b~ Instructions: Clients should !ill in all areas except tl}osc in the ;'Laboratory Use block. 13iomoniloring samples are preserved by storing t~ern at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore. µlease collect and ship in such a way that the laboratory will receive the samples with ample time lo initiate testing within that time frame. Samples shipped overnight on Priday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.. /

r\\ Whole Effluent Sample Receipt Log Page_:to

sample temperature performed using sample Receiving Thermometer: SN 130580085 Date Time Received Received
samplo Project Sample Sample name and description State Comments Received Received by from Temp. (

0C) number number 05-20-16 1225 J. Sumner TVA Courier 2.0/1.0 11480 160520.01 TVA - Sequovah Nuclear Plant-101 TN 05-20-16 1225 J. Sumner TVA Courier 1.8 11480 160520.02 TVA* Sequoyah Nuclear Plant-Intake TN SOP G4 - Exhibit G4.2, revision 01-03*12

I I I I I I I I Page I of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeplzales promelas Client: Tennessee Vallev Authoritv Facility: Seguovah Nuclear Plant NPDES #: TN0026450 Project#: Dilution oreoaration i1Zformatio11: Dilution prep(%) 10.7 21.4 I 42.8 Effluent volume (mL) 267.5 535 1070 Diluent volume (ml) 2232.5 1965 1430 Total volume (mL) 2500 2500 2500 Test oreanism iJzformation: Organism source: In-house culture Age: < 24-hours old Spawn date: Q":>-\\1..-ll... Hatch dates and times*: OS-lie-I~ \\\\.,tie 85.6 100 2l40 2500 360 0 2500 2500 \\ll b'Si-1"'\\*l\\o. t>S-Z.<.:> Transfer vessel information: pH= \\."-\$: s.u. Temperature= ""\\..'-\\. '= oc Average transfer volume: 0.1749 mL County: Hamilton Outfall: 101. Comments: Each concentration was UV-treated for 2 minutes to remove patho!!:eriic Interferences. Test informatio1Z: Randomizing template: ft~\$ . Incubator number and ,~ shelf location: Artemia CHM num6er: CHM882 Dryillg i11fornzatfrmfor weight detennilzation: Date /Time in oven: OS*1.'1,,*1'- *~ Initial oven temperature: '°~*c Date I Time out of oven: ()'i-'l":. *l'- \\.~"O Final oven temperatlire: I'.!\\"( Total drying time: 1.'\\-\\1.o" 11 < I DttUy feeding a11d re11ewa' information: Day Date Morning.feeding Afternoon feeding Test initiation, Sample nu.Pbers used MHSW I renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake I 0 05-17-16 ~OS~o A{ I \\,,CO M \\~~~ Y\\ U.. c:,$l\\o-C\\ l\\:>C15'6.~ os-1~-11,,~ I J. IJ ' 05-18-16 ()\\QO \\~()() ',tt \\.'l..~ XI l\\.QS.11,,.()\\ 11:.Cl~t~.(ft. OS.*1~*1(,6 I 2 05-19-16, v . IJ' ~f rn\\..Jc..... J-i I.J. 00 Lt '1..~2' lloCS I~- 6 ll.. ()<::..lex... 0\\. os.-11.Ht..6 3 v u~ 05-20-16 OK\\~ V1 \\'-\\\\S, \\ 1.'-\\0 V1 l\\oo~ \\S. C>~ li...os. \\Ii. al.. os-L(.-1 li:> 4 05-21-16 C.>. ll\\,\\S }{ \\~\\ , )A l lc.OS1.o*"1. Oli.*~*llo I\\ Of I~ 11 I lioOS"'?.o.o \\ 5

~

05-22-16 or.:,~ ~ \\ '-\\. ~() \\1..SO Y( \\loQS '2.0- o\\ \\lo~"2l)-CJ'l.... o~*l*H'-A 6 05-23-16 Oi'lS II 11. \\:;'\\~ -M \\~S x\\ . l\\oCl~l.0-0\\ \\ (e Cl:i. 7.,Q - 01,.. o~* IC-LbA 7 .,~ \\"2.'!> '5 ~\\ 05-24-16 Colllrol information: U\\l-~o;-.._ A.:ccp1ancc criteria Summmy uf test e11dooi1tts: % Monality:

01.

~ 20~1, 7-day LC;n > l('/'\\7 A vernge weight per initial larvae: o_ '-\\~L. NOEC 1r-o? Average weight per surviving larvae: C.'-\\f-1.... ~ 0.25mgtlarvae LOEC '>I C()I ChV '> JIY\\7. IC1s '>"1001.. Page 26 of87 "SOP AT20 - Exhibit A T20.3, revision 11-01-14

J ~,-T"8 1 'J ] 1 J 1 1 ) J J 1 1 )~....--. Page 2 of7 Species: Pimephales promelas Client: TV A I Sequoyah Nuclear Plant, Outfall 101, UV-treated Date: 05-17-16 Sun*ival and Growtlz Data Day CONTROL 10.7% 21.4% A B c D E-F G H I J K L 0 10 ' 10 lO 10 10 10 10 10 IO 10 lO 10 l f o lo 10 10 ltl lO I 'Q lO (0 co ID ro 2 l \\) to 10 f D lO lQ 10 f 0 lQ 10 to 10 3 !() 10 { t> ID I() I Q IQ ft to IQ lb 10 4 I~ IC) I(} IO f IJ ]() lb 10 10 IQ /() I() 5 It:> 10 lD IQ I{) ID IO to I{) IO II;:) 10 6 l () f b tQ I(} lt) t \\::) t t> ID I \\J f D LO re 7 to **10 ID IO 10 ID lO ID ID lb ID rD A =Pan weight (mg) Tray color code:: .~tb-TlL Analyst: Date: Qi:\\-'UO-lb llo.()b 15.1'3 t"\\.t'O 1~3 IS.Sb lb.'3b lbJ'f 15.~ l,b.-:;1 f'),qcf lb~5 \\l-1.58 B - Pan+ La/lac weight (mg) tC\\.1q. Analyst: Al ~.1t. l'\\.()3 j.).145' ;).\\.3" l}..).) 'A01 d,O.b1 ~'*"~ ~o..<., ~

l,t>.J.1 Date:

~-:-~-l~ C = Larvae weight {mg)= B-A t11-r:r- ~-<<'- Hand calculated. ~ ~ . "{:'2.\\. ~.~S s.s7.. ~ s.ss ~.'\\~ s.01 s.os 1..\\.1{'.) <..1'-\\ S.I.~ '-1.10 s.\\tr.:. Annlyst: Weight per initial number of larvae (mg) = C /Initial number oflarvac Hand calculated. ~.\\)- ~,_., zy ~ rP ~ ""~ ~ /\\.Q ~ if ~ ~ ~ ~ C? ~ ~ ~ '° Analyst: " o* o* . t>" Q" 't)' ()* ei* \\Y o* c* ~- C)' Average weight per . Per~nt reduction (k"-\\&1... II o.S~ initial number.of from control {%) - \\ S*:11~ o.~~ -n.\\oi. lanac{mg) Comment codes: c = clear, d = dead, fg =fungus, k = kiHed, m = missing, sk = sick, sm =unusually small, lg= unusually large, d&r =decanted and returned, w =wounded. Commellts: Page 27of87 SOP AT20 - Exhibit AT20.3, revision I l-01-14

Page 3 of7 Species: Pimep/1ales promelas Client: TV A I Seguoyah Nuclear Plant, Outfall 101, UV-treated Date: 05-17-16 Survival mzd G1*owth-Data Day 42.8% 85.§% 100% M N 0 p Q il s T u v w x 0 to 10 10 lO lO 10 10 lO 10 LO 10 10 I 10 10 ID. I 0 ro lD tu (0 f D 10 IC I() 2 1D ID 10 10 10 10 to 10 co LO 10 'O 3 10 ID 10 J{) '() ID ID 10 IQ 10 IQ (0 4 It:> 10 IO to IO 10 10 'O IQ re IQ ro 5 IC> l \\) Lt) to to l {) /0 tD lD IQ I (J LO 6 J\\) 10. JO. It) J\\)

o I()

ID IQ LO 10 ID 7 JO IQ (0 t {l ID I() l \\) LO lb lO IC IO A= Pan weight (mg) Tray color code:: M ~ ~.ni~ l5.S, fb.3\\ lb.b"t 15.fo1 i~.'fl, 1s~10 lb.40 \\b;~\\ l~.~'t 1s.31 \\'S. z.q 15/~t Analyst: Si,~ Date: Q'-4*'.t~-na B =Pan+ Larvae weight (mg) ~(>.~~* ~l.'f7 'c}.l.1lt )g.i.{° Analyst: Nb Jl3\\ Date: Oy.},,b-lle;

).0.'5' ~l;li w.~ l~*~7 ~b.J~ J'l.&7 ~~*1l C = Larvae weight (mg)= B-' A Hand cakulaccd.

s.o'\\ S.l\\a S~J\\,\\ s.1.'{ L{.~'\\' 4.SS "f.18.:ts"l.. 9~S~ "\\. l.'l. '\\.\\. 2d I{.'\\\\ ~ Analyst: Weight per initial number o[Jarvac (mg) = C / lnitinl number oflarvac. ~ b'V "v'b ~ !)-. 'O tr.. 4 "~ ~4> "bl\\/ Hand calculated. ~ V>o ~ £?. of ~ §: ..)... ~ ~ .)\\ ~ Analyst: o* ti* io~ t)" t>" o* -~. 0 ~* 1::1* \\): \\:)" A**erage weight per Percent -reduction - ct.\\ 1. s.~1.

o. \\.\\i..'-1

3. g.1.

initial number of from fontrol (%) 0- S-Z..i.o O/.\\Slc lan*ac(mg) ~ Comment codes: c =clear, d =dead,. fg =fungus, k =killed, m =missing, sk =sick. sm =unusually small, lg = unusually large, d&r = decanted and returned, w =wounded. Comments: Page 28 of87 SOP A "[40- Exhibit AT20.3, revision 11-01-14

I I I I I I I I I I Page4 of7 Species: Pimep/zales promelas Client: TVA/ Seguovah Nuclear PJant, Outfall 101. UV-treated Date: 05-17-16 Survival a11d Growtlz Data Day 100% Intake Control - Non-ta*eated y z AA BB cc DD EE* FF. -~G.. :),m 0 10 IO 10 10 I~ l() to lC l 10 lo JD IQ /\\) ID ID fl) 2 10 CQ It;, I tJ It) l () l\\) co 3 to 10 10 10 /() IQ IQ lQ 4 '~ ro tD lD ra lG lO ra IQ 5 ID lb I lb f b t () lb to l {J 6 ID } () f O lb Lb f O ID 10 7 ~ '~ \\SI-\\* "l.U. 10 tb { l) l'D iD l '{) 10 I{) A - Pan weight (mg) fll\\~U\\t(I.... ~ Tray color code:: b.1..~ l5.Cb \\5.ee> 15.c'.\\'1 l5.'\\-b 15.'3'l lL\\.qb Analyst: ~~ Date: °"'"'*2.irlb

c;..J fl'4*!fo-I!.

B =Pan + Larvae weight (mg) Analyst: l\\J& ~O,C10 lt\\~ 14.\\3 Jt\\.1\\8 ~\\.l'\\ dbtqs- ~\\.Ol\\ it ~14 Date: 05,~*llt, C = Larvae weight (mg)= B-A Hand calculated. ~.1& "I.lob ~.as t.l.olo $~1~ s.~s "i.loS. (..u Analyst: ~ Weight per initial* number oflarvac (mg) =CI Initial number of larvae 0 l,D. '7 "'Cb Hand calculated. ..... 'b Sf ~ ~ ~ CJ' ~ ~ "\\,g Analyst: r;;,* '\\)" t). C>" <Y Cf ~* . o* A\\'erage weight per Percent reduction 0.4n-1Y..$ 7.. o.s~o Net"" initial number of from control{%) Affu~* lan*ac(mg) Comment codes: c ='clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lg = unusually large, d&r =decanted and returned, w = wounded. Comments: J] JJ SOP A T20- Exhibit AT20.3, revision l l-01-14 Page 29 of87

~., Environmental Testing Solutions, Inc. C11nccrur:11l*1t1r%1 lhpllc~1r hil!IJl,.t1n11irrior f'i11.llniirul11::r1*rlu,*~c,\\ = l 111n\\\\d~l1l hl"\\.1c

- t111i:I cc 10 IO 15.itti

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I lo 10 16.11(1

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(" 11* l!J 1o11n I) 10 ID ll,9J t-: 10 ffJ 15.86 10.1-1. I' Ill 10 16.:16 1; 10 10 l<i.H II Ill 10 15.60 IO 10 l6J7 11,4% 10 IO -15.99 I\\ Ill JO 1r... 1s f, IU HI 14,58 ~* Ill Ill ll:l7 42,8% N In 10 16.JI 0 10 II) IG.62 I' IO Ill IM7 <} 10 10 14.ol6 ij$.6')l, JI JO 10 IS.70 s 10 10 16..10 T 10 10 IG.JI u ii) 10 16.J.I l00% \\' IO 10 ll,}7 \\\\' 10 10 IS,l9 x Ill 10 lj,IJl \\' IO 10 rn.22 HIO"l. (nU1h(' 'l 10 10 ll.06 ,\\,\\ IO

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IS.68 Jiit l<I l{I .15.92 01HfMll IOI: MSD'* D111111tll's ~JSD v11l111*.: O,n705 ('.\\ISO* PMSIJ: 14,6 l!!.!nk Dunnrll4! l\\ISD vttlU!! 0,062J PMSO: ll.9 TVA I Sequoy11h Nuclenr Pinnt, Outfall 101 Ma~* 17-24, 2016 Pimcp/111/es promcl11s Chronic Whole Effluent Toxiclly Test El'A-821-lt-02-0IJ, Method 1000.0 Qunlily Co11trol Verlfici1tlon of OJttn Entry, Caleulntions, and Statlstlc11l Analyses Nol for Co11111li1tnN /\\~s:cssnh!nl, lnlt.?m:tl Laboratory QC 11~l'Jn+l.;1m1r-J.Jn1e"d)!l1ltr11Jll \\\\'clt,hl/S11r.l,lni,:

\\h*.211niili!hlt C.'t"*rntrcnlc1f**oirbllnn Wrlchtllnlllllnoml*rr

"rlR,l1ltniiU nA'*R nt1l'l\\hu11r1:i:n:rC'(nr1~*

1'i111,l\\ln~n1m1lirtrif 1~11*~****,.., 1~111'"' 11ff,lnilt'flt1UI hrt.w(111~) "~""r11.,111t*1.1 11.19 l.73 057J (l,l7J 2U.*I~ .*.55 0.5~5 0.)80 56 11.555 !l.O*I l.65 O.l6l . O.l65 ?1.2*1 6.28 0.1*28 0.(17,8 20.76 4.70 0.HO 0,.110 19.?4

l,21.

. 0.421 0.~82 11 l

OAil 19.0J

~.Sl 0:4gl 0.485 !1.45 5.S2 0,5l2 0,5S'1

?l.J2 l.46 O.l~6 U!i4Ct ll.21 5.85 0.585 o.m 7.1 0.585

'l:?.07 l.93 o.~!JJ 0.5?.l 20.67 5,07 M07 1),507 21.ili 5.05 0.505 0.505 21J.69 UIJ 0.470 o.m Jl,8 0.470 21.59 6.lol 0.6lol O.lilol

m.21 5,6}.

0.563 .O.M l0,6(o l:O.'l 0,509 0.509 21.*17 !i:Jr, 0.516 0 5!6 4.9 (I.SIG 21.76 l.1*1 ll,ll4 0:514 21.ll S,M 0,j64. O.S6°I JS.HS

l.'J!J 0.*1)9 0Al9 20.25

{SS l).olSl 0.456 l.6 OA$S 21.18 4 78 o.. 178. O,ol78 20.83 4.12 1).452 OAS2 20.87

- 1:!1 0,4jJ 0.. 153 J0,19

. 4.82 IJ.4S2 o..i8J 19.l7 4.28 OA28 0461 6,! UA:!R 20.82

1.91

.0.491 0.-191 20.00 l.'18 ll.J78 0.378 1966 HO ll.ol60 0412 8.l O.ol60 19.93 4.0S

o.40S o..ios 19,98
1.06 0.406 IJ.*!06 Minint111u S1~nll1<;ml Dill"oo:rn:c P*m:nl r.im<muni Sli:ninC0111 DilT*l'ale*

P(Ojrlf n11111b*r1 ___________ 1_1_~1JO_ Mnn1unh11l ,\\lt>lnurl"hl/ C11flncfr111nf l'rtHnfrtdudfnn(lllm I'/,) tn111111nu111htt 1111bl/11a 1Ut1'""'"*il ,~1ntrd t'/*) lan:u:(ltr~f f<Ul"ill*llo*t..,l<1r10"*ffl l'l*I rno.o MMO M Nol UJIJllitnblr 100.U 0.4~l 11.2 Nol np1dic111ik 100.u II.SSH 7.1

15.7 100.11 O,SJft f 1.8

-JI.* 100.0 0,526 '4,9 .!J.I lllU,O (1;~56 J,6 $.~ 100.0 U.4M 6.1 3.ll

!Oii.ii 11.412 8,J 14.S PMSD 1umwurta(l'5lpre<iiio<I '[110 l'MSD " ill< mlnm1u1u PCl"""l dill"cr<tl<< b"ll'c'<m 11l.i ***lfOI""" ""'""I 11111 CIUI b* dHl.ln:d.m1i11ico!ly 6i!llliRCILOf In. 1'11010 dllu

ICU)' l<JL La11ct rMSD boWld dc1orn1incol by USCM ( I01h porccnliloJ = 12!\\

MSDu MSDe MSB MSE F-Prob Dunnell's Test

0.5

. 02 0.1 0.0 O-.c>-~--c.---

5. 18'

intake 0.4123 0.8553 0.4123 0.3780 0.4600 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.05}

g c: c u

Ha.rdncss (m° CaCOi/L}
Tem erature (0C).

Alk.-ilinily (mgCaCOYl.)
Hardness

TR' citlorim: (nig!L).
Te~perature ee>

Alkalinity (maCaCO,;/L)
Hardness (nio CaCO:;/L}
TR. chlorine (mg!L}

Temperatures perfom1ed a1 lhc time of test initiation, renewal oncm1ination by the analyst identified in tlic Daily Renewal Information table located on Page I.

Alkalinity (mgCaCO:;/L)

TR chlorine (mg/L)

Alkalinity

Temperature (°C)

far.ameter

.* C<induttMty (µmhos/cn:i)

Alkalinity (mg CaCO;;/L)
Hardness

Temperature (°C)

Alkalinity (mgCaCO.;/L)
Hardness (mg CaCOJIL)

Temperature {°C)

3.

Temperatures performed at the.time oftest initiation, renewal or termination by ihe analyst identified in the Daily Renewal Information 1able located on Page I.

lnll (SU) llOlml!ll.l

Conrrol,

** Cond11c1M1v Cumbosfcml Non*trente~

Cl

11111 ISUl 8S.6'V*

Totnl n~sldu11I Ch1otlne lmrill.

'J'otlll ~esidual Chlorine lnu!lL

- 318 61 88 24.7
- 7.44 1;s 321 -

?4~8

'***7.39

7_30 7.24

- - 7.50 7.9 7.8 7,8 7,8 7.8 7.8 7.8 7.8

307 310 322 62 61 88 86 2*1.4 24.8 24.()

1.64 7;01

1.2s 7.8 7,9 7.8 7.8 7.8 7;8 7.8 7.8
319.

6:L 88 88 24.:?

1.6S 1.03 7.23 7.:24 7.6l

1:65 Ml

m 24.5 24.9 24.7 24.ll 24.3 74.7 24.7

?4,8 7;S6.

7.03..

2'1.5 JO 2li,.:7

- 7.64

7.60

i4.8 24.4 24.8 7.63 7.66 7.06 1.18 7.38 7.62 6.97 7.23 7.9 8.0 7.8 8.0 7.9 7.9 7.9 1.9 196 197 199 196

i4.3 7.28 7,9 24.l 7.28 s;o 24.7 7.31 8.0 24.4 1.30 8.1 24.4 Project nuhlbcr: ______

zoo*,

?86 24.6 24.9 24.4 7.00 7:-35 7.32 7.8 8.0 7.9 2S7' 2.\\.6 24.9 24.4 1.00 7.34 7.32 7.9 8.0 7.!l

?Oil 24.3 24.9 24.6 7.07 7,3'.I 7.33 7.7 7:9 7:9

'l":~j!:J ~.

Test initiation and feeding, MHSW County: Hamilton Outfall: 101 Commell/S:.

Selenastn1m batch:

61.

07.

S:20'}1;,

> 1001.

_ f{f.. '1 iq;;-~ I~

Survil)a/ a11d Reprodli.ction Data Repiicate number Day I

~

o

a.

~

'4"

O 0

,L-

L*

a*,

n 0

Adult mortalit)'
L '-

s.

~

u;

L-L-

-

x.

.?r (offscuin~ c:urlCd D\\'Cr with ;,duS: du.ijr-'! tr:rnsrcr).

07.

. L

~.::~--;

z 3

l..._

.\\\\

z

6.

o 0

>~

\\...._

01.

3

Cmici!irttntiou:

r.

-\\_

o 0

07.

- .~:~.-.

n.

Total young produced

?

(")_

.s 3.1-2&

I

~

3 l

4
I 5

IJ 13 6

0.

11.

15.

I s

o.

0.

I
1 3

o.

- 5
"6' 7

I 4

o.

o o*.. o

I s
I

12.

o 0

17

14.

1.1

I 5

I S*

13.

o.

0.

l s
- 10 4

II)'

12 12*

15.

1911

33. J4*

3 0

0.

o.

1

o.

o tl.

o

0.

o 4

:.).

I

5

12
- 11 12.:

a 0
.o.

Joi.

1

0.

3

1: 5.

12

I'

3.

J I~

0.

30.

z1 26. lK 100% Intake Tutnl 8

13

13.

o 0

o 0

0.

I 5

- l s...

6.

()

12.

o 0

1tc illcutc number
I 5

I
I:!.

o u

o.

I

)

I
I 10

10.

19.

o.

I
I Joi 14

J7 33 8

5.

I.II ll"
u
o

32.

10 1'0101 0

I 45 t(i 117 n *

.o.

I I) 0 IS 31 Totpl 0

18 128 0

o.

12.8 37 35 34 37 33 33 IOO J4.7 4.5

Start Date:

m,ooo 32.000 32.000 33.000 3:;.000 29.000 30.000 32.000 35.000 34.000 32.000 36.000 3i.. ooo 35.000 32.000 29.000 31.000 36.000 33.000 37.000 35.000 34.000 37.000 33.000 33.000 32.000 28.000 31.000 29.000 30.000 29.000 32.000 32.000 Transfonn: Untrnnsformed 1-Tailed Isotonic Conc-o/o Me;in N-Mean MG:.n Min Max CV"lo N

g 0,5 g 0.4

g. 0.3.

5. 54

Hardness (ini; C~C03/L)
TR chlorine (mg/L)
Tern* eraturc (°C}

Temperatures perfonned at the time of test initiaiion, renewal or 1cm1i11ation by the analyst identified i11 the Daily Renewal lnfommtion table locntcd on Page I. Alkalinity. hardness and total residual chlorine perfom1ed by Lite analyst identified on the bench sheet specific for eacl1 analysis and lranscribed 10 Ibis bench sheet by: +*

Alkalinity (ma CaCO;/L)
Hardness (me CaCO;/L}

Alkalinity

Hardness (mgCaCO:;/L)
TR chlorine (mg{L)
Tern erature (°C}

Hardness (mo CaC03/L)

Temperature r'C)

Temperatures perfonned at !he time oftesl initiation, renewal ortem1ina1ion by the analyst identified in Elle Daily Renewal lnfomialion table localed on Page I. Alkalinity, hardness and total residual chlorine perfonned by the analyst identified on the bench sheet specific for each analysis and transcribed to !his bench sheet by:

. *
Initial*:

2S.0 oH (SUI 7.28 DOlnm/J,l 7.B CunducUvltv (umhus/cm)

Onvl Dn,*3 Dnv4 Plnul:
Initial -

lnlllnl -

lnllldl

- Tnlllal -
1;1nnl 1.53 7.39' 7.21 1.30 7.56 7.64 7.23 7.SO 1.S6 7.8 7,9 7.8 7.8 7.8 7.8 7.R 7,8 7,7 313 307 310 31~

!70

7.18 -

!47

?01.

2U 7.SI 7.Sl

is.o 14.8 7,Stl 1.S6 7.16 7,21 1.53

.** '7;9

7;9 197 200 188 199 73 73 12 76

24.8 l*rojecl nu111bcr: ______

1.11 7;8 7.S l93 24.8 25.0 7.46 7.08 Ul 7.9 284.

?4.9 24.8 7.44 7;05 a;o 7.9 208 14.9 24,8 7;40 7.06 8.1 8;0 191 lS.0 l4.8 7.40 7.11 8.[

?U4 24.8 lS.I File: sqn101_051716chem Entered by: J. SuZfer Reviewed by. -6lf-

_;__~, > "

,.**~*.'.-*.

f't..f

i~. /

-,* "*i.

"*w**

---~

pH of Deionized Titrant Begin water reference standard ml

""'3~--1 Time completed ~--~'

D).ll(S+D)/21} x 100 (acceptable range = +/- 10%)

t !JO 31_.'-I-1.(3., 5,-.:t 10.7-ln f 06\\t.\\\\v R 4-3.(. H~.e, 5,7

iJ 11-.tf-1-3-"l.. 5:6 ln J..

(acceptable range 0.0180 - 0.022U) -

D) /l(S+D)/2)} x !00 (acceptable range== 10%)

CS\\

t-3

.w 6'-t

z...

ml ml ml

lOO 100 (o.\\

s. ample mensuremellts:

J-3 l \\.., hC:.\\ lo. 0 L-rf\\/'ACC\\N\\\\.t\\1.hJ t qs.'1-L\\-S.q...3. 0 lo+

_,'2>-,

TQtal

h. ~

i t**1-

1-I

Date analyzed O!j'- a.J -ffl Hardness (Sl\\12340 C-1997)

r:

c 1 rtrallt 1wr11u1 rtJ' mul mu/11p rer fo eterm111at1011:.

o. o~oo laboraton* co11trol standard:

.sample ID volume Begin End Total Multiplier number (ml) ml ml m:I OS \\°6LIJ}b mttsw Sf;

t J~.q 4-b.a Y.. ~

2.

~s.._ *o~ 1"'1 *a eJ.o

~o.o g.,

r 1trmrt 11or111a rty a,11t. 11111 tID 1er t. etermmat1011:

t.

Total Multiplier

l~.. o. 'J-).1 s.1 q..o.o s 1/0 Duplicate (B) i

i.S"*"\\

. r;. os~fl4 (should 'be= 0 mg CaCO~/L)

I

2.

6\\

>_.~

2.3 q~

~l 3.<-1

18. r

1 ll'A Duplicate (B)
,I

l.f) ml

).l. (e

(Jlcceptablc range

Sample hardness (B) lV!easured spike value (MV) o/o R =:'MV I SV x 100 (l)Ig CaC03'L)

Begin End Total Hardness Sample number Samele ID (mJ) ml-ml ml Multiolier (mg CllCO.VL)

Blank TV=ND (should be = 0 mg CaCO.y'L}

7, I~ oS \\il.ol.>c;.

ti\\

Q \\

~A

volume Begin End (mg CnC03/L)

\\.\\* \\~

i:::

- - - - ~******~**********
        
        ~*************-

- - - - ~***********

0.58 0.65 0.81 0.70 0.61 0.69 0.72 0.69 o.78 0.70 0.7S 0.71 0.64 0.70 o.os 0.08 0.10 0.09 0.09 0.08 0.08 0.08 Pimephales promelas Chronic Reference Toxicant Control Chart

CT-54.10

s. 10 * ~tondard deviation corresponding to th~ 10"' percentile CV. is. 10 *0.12)

c:.c 0

.;:; s RS 0

c:

u....

c.

MSO ~ Minimum Significant Difforence PMSO = Percent Minimum Significant Difference MSD PMSD er

re ~*,: * ';3 (c c

'as~o Vi

° mce k

o. '5.cr1 NOEC

0.25 mgilarvac LOEC

10.

IJ.O.'~), p.\\.CJ'\\ 1g.!)1 ~.S-lt Je\\."Tl ~o;o~ ~(Ml '-.O~Dq ~0.0~ It\\. f&~ 11\\.'IO Jl\\.'f}.

y;

~.'Al lt\\ *. 11 I'\\. I\\~ 1i.1~ lb.~~

~

.\\

~

\\ = Pan weight (mg)

O~:t-'1.'~-110 -

~

Pcrcci:it numbc.r oflarvac (mg) reduction O~O&tJ

15.99 IS.9l 16.SS MSD*

?.81 0.'168 15.99 o.68 0.340 16.84 0.85 0.425 16.50 0.59 0.59Q 17.62 1.07 O.l57 Minimum Si&OIJlcant 01fferenco Porcenl Minimum Significant Olfforonco Mun'llrH'l1!1l/SunMri1 (otffl.dtnl of vul.1llqn Wtltllt(lt1U11t11utnbtrol nu1ri~1rorl.1rv11 (1111l)

"M"'

0.406 l.6 0.396 0.4Dl 0.422 0.393 0.393 5.0 0.383*

1211.

900
rnso 5117/2016 512412016 1.0000 1.0000 1.0000 0.9000 0.9000 0.6000 0.2000 Me:tn 1,0000 1:0000 1.0000 0.9750 0.9000 0.5750 0.2000 Auxlli:i!'.l Tests 2

Max CV%

o l.8'2817 9.48773 0.76732 2.96364 0.07814 3

3.355 684.091 606.329 734.542 6.8 EC10 3.718 730.3 "*662.93. 775.211 0.7 EC15 3.964 763.229 703.338 804.683" EC20 4.158 790.455 736.489 829.706 O.&

i

~-..

!.>

une:ir lntorpot:otion (200 Res;imptos)

indicates IC estimate less than !he t.owesl concentration g 0.6 0.5

3. 12 1500

J

Mkalinity (m~ CaCO;/L)
Hardness

Temperature (

Temperature (°C) pH {S.U.)

Temperature ("C) pH{S.U.)

Temperature (°C}.
pH(S.U.)

T-emperature ("Cf pH(S.U.)

Tempernture (°C}

Temperature {0C)

1..

1 l

... _,_.-~

Conductivity

Temperature (oC}

Temperature (OC) pH (S.U.)

Temperature (oC) pH(S.U.}

Temperature

Temperature (oC)

fem craturcs performed at the* time of test initiation, *renewal or tem1ination by the analyst identified in lhc Daily Re newel lnfom1ation table located on Page I.

*....-J

i...

- - - - ~*******************~*** ************
- ~*~*****~*-**************************

- - - - ~***************************************************************************************

- - - - ~***********************************************

- - - - ~*********************************~***********************************~***************,

s._,.

- - - - ~**************************************~****

--* ~--~**~**-* -***-**.:...._**-**-**-**!"""- ---*-
- -*---**-~~-**-**__..:,**-*... _,, __ -**-**-**-

01.

<:80%

s. 07-

1.

re-rr I

K.

07.

2.

3.

0.

£

arried over with adult during tnmsfi:r).

r-.

(J a

0 "31\\

,'1,..

~:iL

5.

0. 0

r=T*~

("")

5

Survival alld Reproduction Data

0.

s.

07.

0.

12

15.

-i -

0.

12 120 5

17.

8 9

o

'.l 4

121 5

5 3

r 0

o 0

o -

2.

JOO 32.l 4.5

s 4

Variability Study (USEPA, 2001 n: US GPA. 200 I b).

Transform: Untransformed Rarik Conc-m2/L Mean N-Mean Mean Min Max CV%

1000 29.000 0.9177 29.000 27.000 33.000 6,082 10 67.50
1200 12.100 0.3829 12.100 8.000 15.000 18.866 10 55.00
1400 4.000 0.1266 4.000 3.000 S.000 26.352 10 55.00 Auxilia!}'. Tests Statistic Kolmogorov D Test indicates non-normal distribution (p <- 0.01) 1.17686 Bartlett's Test indicates egual variances !E =- 0.22}

> -e 20

c.

o.26293 0;3891 15.0663 500 1000 1500 Dose mg/L

Sample ID:

1000 29.000 0.9177 29.000 27.000 33.. 000
1200 12.100 0.3829 12.100 8.000 15.000
1400 4.000 0.1266 4.000 3.000 6,000 AuxiliaryTest5 Kolmogorov D Test indicates non-normal diStributfon (P: <= 0.01)

I "Cl ?Q 2 -

Alkalinity (m CaCDl/L)
Hardness i:naCaCOJ/L}
Temperature (0C) pH(S.U.)

-Temperature (°C) pH (S;U.)

Temperatm*e ("C) pH (S.U.)

remperacures performed at the time oftest initiation. renewal or termination by the nnalys1 identified in tl1e Daily Renewal lnfonnation cable located on Page I. Alkalini~y and hardness peTformed by the analyst irlentiiicd on che bench sheet specific for each analysis and transcribed to this bench sheet by; ~-

Alkalinity (mo CaCQ3/L)
Hardness (mo CaCQ3/L)
Temperature

Temperature

Temperature

Tem~rature

Temperature

Temperature

Temperatures pcrfonncd at the time of lest initiation. renewal or terminalion by the ana)y5l identified in the Daily Renewal Information table located on Page I. Alkalinity and hardness performed by 1he analyst identilied on the bench sheet specific for each analysis and transcribed to this bench sheel by: -...,/(,,___

ML16256A778

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ML16256A778

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Dear Ms. Hall:

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