ML15349A895
| ML15349A895 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 12/10/2015 |
| From: | Schwarz C Tennessee Valley Authority |
| To: | Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources |
| References | |
| Download: ML15349A895 (110) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 10, 2015 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR November 2015 Enclosed is the November 2015 Discharge Monitoring Report for Sequoyah Nuclear Plant. Per your request, Attachment 1 is also enclosed which summarizes the average monthly flow from Outfall1101 for June - October, 2015. The Turbine Building Sump was aligned to the Yard Pond November 20 -
27, 2015. Turbine Building Sump monitoring data is summarized in Attachment 2. The final report for Toxicity Testing conducted October 18 - 23, 2015 is also enclosed as Attachment 3. Attachment 4 shows the corrected October DMR page for l01T (based on receipt of the final Toxicity report).
There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.
I certify underpenalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gatherand evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, Sequoyah Nuclear Plant Enclosu res cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk Chtaog,4State caleOffice Building,AeuTnese Suite370_0550 3Washington, DC 20555
- PERMrITEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
Address P.O. BOX 2000
.._(.IJNTEROFFICE OPS-5N-SQN)
S TN0026450 I[101 G F -FINAL.
SODDY - DAISY, TN 37384 S PERMIT NUMBER I[DISCHARGE N-UM-BER] DIFFUSER DISCHARGE Facili_ TVA - SEQUOYAH NUCLEAR PLANT EFFLUENT Location HAMILTON COUNTY I MONITORING PERIOD I MO I DAy NO DISCHARGE [*
ATT-N:Millicent Garland From!LYEARI 15 11 01 ILYEARI Tol I1 MO 11 I130 DAY NOTE: Read instructions before completinpa this form.
PARAMETER QUANTITY OR LOADING "QUALITY OR CONCENTRATION NO. FREQUENCY! SAMPLE
_________-EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********** "********** 32.7 04 0 30)! 30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ***** **** ** **** **** Req. MOn. DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE **** ****** ********** 20.9 04 30 / 30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT ***** **** .. ******** 30.5 DEG. C. CONTI CALOTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE **** ****** ********** 2.4 04 0 30 / 30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 1 PERMIT ********* **. *********5.0 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX ___ _NUOUS TRAMNFLOW,IN CONDUITpLNOR THRU MESRMNSAMPLE ***.. J
- 03 ......
- a.....30 /30 R*CORDR 50050 1 0 PERMIT ***** Req. Mon. MGD ****************CONTI RCORDR EFFLUENT GROSS RQIENTDLYMXNUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1360 ***** 03 03 0 30 / 30 CALCTD TREATMENT PLANT MEASUREMENT 50050 1 0 "PERMIT Req. Mon. ***** MGD ********* **** MGD CONTI CALCTD EFFLUENT GROSS VALUE REURMN OAGNUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ***** **** * ***** 0.021 0.029 19 a 19 /30 GRAB MEASUREMENT 50060 1 0 PERMIT ***** **** ** **'**0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE *****0.2 62** 0 30 / 30 CALCTD CHANGE MEASUREMENT 6 82234 1 0 PERMIT *****2.0 DEG *************** CONTI CALCTO EFLEN ROSREQUIREMENT DAILY MX C/HRNUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thes document and all attachments were prepared under my i* -TELEPHONE DATE tirection or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the Site Vice President person or persons who manage the system, or those persons directly responsible for gathering the information, the intormation submitted is , tothe best of my knowledge and belief, true, i Vc P es n-42 83701 15 423____843-7001_______15__12___10_ 2 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED ___________________________I________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injection occurred: Flogard MS6236 (max conc. Was 0.05 mg/L, limit - 0.20 mg/mL),
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fot PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) rm Approved.
DISCHARGE MONITORING REPORT (DMRJ SB 1 I Name "FA- SEQUOYAH NUCLEAR PLANT 18No. 2040-0004 Address P.O. BOX 2000
- -.- INTEROFFICE OPS-hN-SQN)
TN0026450 101 T F-FINAL SODDY - DAISY TN 37384 ._ PERMIT NUMBER - DISCHARGE NUMBER] BIOMONITORING FOR OUTFALL 101 Fac*jj_ TVA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY !MONITORING PER1OD EFFLUENT ATTN:Millicent Garland From 15 I YEARI MO 11 I DAY 01 I Tol YEAR I MO 15 11130 DAY **NO DISCHARGE Z*
- NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUCENY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 1C25 STATRE 7DAY CHR SAMPLE ***** ****
- Monitoring **** 23***
CERIODAPHNIA MEASUREMENT Not- Required
-° 23_____ _
TRP3B 1 0 PERMIT ********* **42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS RQIENTMINIMUM ANNUAL 1C25 STATRE 7DAY CHR SAMPLE ******* Monitoring ********
PIMEPHALES MEASUREMENT Not' Required
-" 23_____
TRP6C 1 0 PERMIT ***** **** .. 42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS RUIENTMIMINUM _____ANNUAL _ _
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT
-REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER -ICertify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE
-direction or supervision in accordance withea system designed to asaure that qualifiedt._/"2 ,-\f*
Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the L.
person or persons who manage the system, or those persons directly responsible for gathering _ -_'"_ e Vice P r____.__. _____ 423 843-7001 15 12 10 the information, the information submitted is, to the best of my knowledge and belief, true, ________________________
Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al/attachments here)
Toxicity was not sampled in November 2015.
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Formi Approved, Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SB01OM No. 2040-0004 Address P.O. BOX 2000
---. (INTEROFFICE OPS-5N-SQN),
[ TN0026450 ][103 G [F-FINAL SODDY - DAIS.Y, TN... 373._84__. . . . S PERMIT NUMBER DISCHARGE NU0MBER] LOW VOL. WASTE TREATMENT POND F._aciji_/ TV._A. - S..EQ._.UOYAH N UCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATl-N:Millicent Garland From LYEAR15I MO 11 010 m° DAYI 15 II11 To LYEAR MO I1301 DAY *
- NOSHREI ODSCAG I
- NOTE: Read instructions before completin~q thils form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_________ _______________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ***** **** *7.0 *****8.7 12 0 5 /30 GRAB MEASUREMENT 00400 1 0 PERMIT **** ***** ** 6.0 *****9.0 SU ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ......... ** *...** 15.8 15.8 19 0 1 / 30 GRAB MEASUREMENT 00530 1 0 'PERMIT **** ***** ******30,0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MIX MONTH OIL AND GREASE SAMPLE **** ***** ** <5.0 <5.0 19 0 1 /30 GRAB MEASUREMENT 00556 1 0 PERMIT ***** **** * ***** 15.0 20.0 MGIL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH____
FLOW, IN CONDUIT OR THRU SAMPLE 0.972 1.151 03 0 5 /30 INSTAN TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD .***************ONCE! INSTAN EFFLENT ROSSREQUIREMENT EFLETGOSMO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEJTrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thia document and all attachmenta were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified / ' *..
Ch ristopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the VcePeL p)erson or persons who manage the system, or those persons directly responsible for gathering -,---Slte Vc r e 2 4-01 15 1 Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, 423____843-7001_______15__12___10_
accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA{ NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) 11/20/15 - 11/27/2015.
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
AddreSs P.O. BOX 2000
- -- INTEROFFICE OPS-5N-SQN).
[ TN0026450 J 110 G F-FINAL SODDY - DAIS..Y, TN..373.84__. . . . I PERMIT NUMBER DISCHARGE NUMBERI RECYCLED COOLING WATER F._acJ* TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY _yMONITORING PERIOD EFFLUENT YEARl MO DAY YEARI MO DAY "'N ICAG FromJ 15 11 I01 To-l15 11- 30
- OOARE @
- ATTN:Millicent Garland NOTE: Read instructions before completing this form.
PARAMETER *. /QUANTITY OR LOADING 1QUALITY j-EX OR CONCENTRATION NO. FREQUENCY SAMPLE OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE **** ***** ** **** 04***
CENTIGRADE MEASUREMENT 0 00010 I 0 PERMIT ********* *********** REPORT DEC C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous TEMPERATURE, WATER DEG. SAMPLE ***** **** *04* ****
CENTIGRADE MEASUREMENT 0 00010 Z 0 PERMIT ********** ********** 30.5 DEG C CONTIN CALCTO INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE *******04**
UPSTRM DEG.C MEASUREMENT 0 00016 1 0 PERMIT ********* ***** ***** 5 - DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous FLOW, IN CONDUIT OR THRU SAMPLE ********** *********
TREATMENT PLANT MEASUREMENT 03
- 50050 1 0 PERMIT ***** Req. Monl. MGD **************** CONTIN RCORDR EFFLUENT GROSS VALUE RQIENTDAILY MX UOUS CHLORINE, TOTAL RESIDUAL SAMPLE **********19****
MEASUREMENT 1 50060 1. 0 PERMIT ********** *****0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE ********** *********
CHANGE MEASUREMENT 04 **
82234 1 0 PERMIT *****2 DEG C ******** ********* *CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER - Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE
_____________________________ direction or supervision in accordance with a system designed to assure that qualified *.
- Christopher Schwarz personnel properly gather and evaluate the informaTion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering it VcePe_ nt-43 4-70 15 12 0 the information, the information submitted is, to the best of my knowledge and belief, true,42 83-0 1 5 12 1 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
______________________________i nformation, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUBR YARM A TYPED OR PRINTED _________________CODE/
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
N._are TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMAR)
(*SUBR 01) OMB No. 2040-0004 A._ddre._ss P.O. BOX 2000 NTEROFFCE OPS-5N-SQN.)
.(I- F - FINAL SODDY - DAIS.Y, TN.._373._84__ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER F._acili~y TIVA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY [ ~MONITORING PERIOD EFFLUENT tYEARI MO T AYI IYER M DAY NO DISCHARGE jj ***
A-TTN:Millicent Garland Froml 15 11 01l Tol jjL 1 J ***
NOTE: Read instructions before completingl this torm.
PARAMETER QUANTITY OR LOADING 1QUALITY __ __
OR CONCENTRATION
_ _- __ _ _ __ _EX NO. FREQUENCY OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 1C25 STATRE 7DAY CHR SAMPLE ******** ******** ** *5*5*********
CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 PERMIT ******** ************ 42.8 ****** ***** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC26 STATRE 7DAY CHR SAMPL*E ************ ***** ****
PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT ******* ******** ***42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT
,REQU1REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT' REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMITr REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. eased on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best ot my knowledge and belief, true, 423 843-7001 15 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 'YEAR MO DAY AREA NUMBER TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01 ) 0MB No. 2040-0004 Address P.O. BOX 2000
- . INTEROFFICE OPS-5N-SQN)
I TN0026450 II118 G ]F-FINAL SODDY - DAISYTN 37384 I PERMIT NUMBER I[DISCHARGE NUMBER] WASTEWATER & STORM WATER Faciliy. TA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY MflNITflPIN* PlPCfIfl 1 EFFLUENT From 15 111 101 Tol 15 11 3o -*NOSHAGE ATTN:Millicent Garland NOTE: Read instructions before completingq this form.
PARAMETER QUANTITY OR LOADING
________IEX IQUALITY OR CONCENTRATION NO. FREQUENCY OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ********************
MEASUREMENT 19 00300 1 0 PERMIT ********* **2 ***** **** MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM _______WEEK SOLIDS, TOTAL SUSPENDED SAMPLE *******************
MEASUREMENT 19 00530 1 D PERMIT **,* **** .. **** ******10 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE ********** *25* ****
MEASUREMENT 2
!00545 1 0 PERMIT ********* ** ******** ML/L ONCEI GRAB EFFLUENT GROSS REQUIREMENT" DAILY MX. MONTH FLOW, IN CONDUIT OR THRU SAMPLE ***** ****
TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. Req. Monl. MGD ***************ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAG DIY XBTC SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER
~~lCertify ire~~~~d under penalty of law that this document and all attachmants were prepared under my TELEPHONE DATE Christopher Schwarz
- ction or supervision in acco rdan ce with a system designed to assure. t hat qualified*..
personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persona who manage the system, or those persons directly responsible for gathering 6
- t .. *n L
it Vice P_ t__42_83701_5 12 1 President Site Vice ~the information, the information submitted is , to the best of my knowledge and belief, true,Pesen42 83-01 5 12 0 SiePesdetaccurate, ic and complete. l am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
- information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CD COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period
Attachment 1 Flow in Conduit or Through Treatment Plant -
Monthly Average Flow Month Monthly Average Flow (MGD)
June 1757 July 1788 August 1788 September 1788 October 1803
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Turbine Building Sump Monitoring Data During November 2015, the turbine building sump was discharged directly to the yard drainage pond from 11/20/2015 to 11/27/2015. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1A.2 of NPDES Permit TN0026450.
Parameter Daily Minimum Monthly Daily No. of Samples
__________________ Average Maximum Flow 1.689 2.145 2 pH8.05 s.u. - 8.59 s.u. 2 O&G -<5.0 mg/L <5.0 mg/L 2 TSS -<13.5 mg/L 22.0Omg/L 2
Attachment 3 Toxicity Testing October 18 - 23, 2015 Final Report
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: November 16, 2015
- 1. Facility / Discharger: Sequovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450 4: Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): L257
- 7. 1Q10:3,9
- 8. OutfallTested: 1.01
- 9. Dates Sampled: October 18-23,2015
- 10. Average Flow on Days Sampled (MGD): 1794. 1825, 1801 11I. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: October 20 - 27, 2015
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids ( Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101" 10.7, 21.4, 42.8, 85.6. 100 Intake: 10__0_
Pimephalespromelas: UV treated Outfall 101: 10.7, 21.4, 42.8, 85.6. 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC25 = 42.8%
- 17. Test Results: Outfall 101: Pimephalespromelas."IC 2 5 > 100%
Ceriodaphniadubia: IC 2 5 > 100%
UV treated Outfall 101" Pimephalespromnelas. IC 2 5 > 100%
Page 1 of 98
- 18. Facility
Contact:
Millicent Garland Phn#:(2)8361
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone Ii: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Exposures to samples collected October 18 -23, 2015 from Outfall 101 resulted in no toxic effects to daphnids. Minnow survival was significantly reduced in minnows exposed to the full-strength effluent. Pathogenic growth was also observed in the gill area of minnows in the full-strength effluent.
The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids and minnows to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival Was improved by two
- minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference may have resulted in the minnow mortality observed in the full-strength effluent.
Page 2 of 98
METHODS SULMMARY Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)
Sample Time (ET) Time (ET) Temp. TRC* Time (ET)
ID Collected Received (°C (rag/L) Last Used By 101 10-18-15 0645to 101-512 .,.1 <.0 10-20-15 1155 10-19-15 0545 1-91125 .819t <10 10-21-15 1100 Inae 10-18-15 0715 to 1-91125.6 <10 10-20-15 1155 Inae 10-19-15 0615 1-95125.6 <10 10-21-15 1100 10-20-15 0706 to 10-21-15 1230 .A._1,9 <0.10 102-506 11 10-21-15 0606 10-23-15 1055 Inae 10-20-15 0730 to 1--5120.6 <.1 10-22-15.15 Inae 10-21-15 0630 1--5120.6 <10 10-23-15 1055 0707 to10-24-15 1057 101 .10-22-15 0770 10-23-15 1250 2 . 4 , 1 ,4 * <0.10 1.0-25-15 1055 10-23-15 0607 10-26-15 1055 10-24-15 1057 Intake 10-22-15,0728 to 10-23-15 1250 0.8 <0.10 10-25-15 1055 10-23-15 0628 10-26-15 1055
- TRC =Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainer's. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: .Samples from Outfall 101 and intake were warmed to test temperature (25.0+/-+1.00 C) in awarm water bath.
Aliqiuots of Outfall 101 and Intake samples were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aciuatics.
Inc.) for 2 minutes.
Page 3 of 98
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: In-house Cultures Aqiuatox, Inc.
- 2. Age: 18.70 - 19.92 hours0.00106 days <br />0.0256 hours <br />1.521164e-4 weeks <br />3.5006e-5 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static. Renewal Static. Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 _1
- 6. Test Initiation: (Date/Time)
Outfall 101 10-20-15 1155 ET 10-20-15 1000QET UV Treated Outfall 101 10-20-15 1142 ET
- 7. Test Termination: (Date/Time)
Outfall 101 10-27-15 1113 ET 10-27-15 0904 ET UV Treated Outfall 101 10-27-15 1052 ET Mean = 24.9°C
- 8. Test Temperature: Outfall 101: Mean = 24.7°C (24.2 - 25.2°C) (24.5 - 25.30 C)
Test Temperature: UV-Treated Outfall 101 : dean = 24.8°C 24.2 - 25.3°C)
(C
- 9. Physical / Chemical Measurements: Alkalinity, hardness. total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinneyville. CA).
Page 4 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench .Sheets)
- 1. Results of a Pimephalespromelas Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20--27. 2015 using effluent from Outfall 101.
Test Percent Surviving Solutions time ihterval
(___ used
[ .1 [4 1 I
____ -days)
(% Effluent) 1 2 3 5 [6 7 Control 100 -100 100- 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 98 98 98 85.6% 100 100 100 100 100 100 100 100.0% 100 100 98 93 83 78 75 Intake 100 100 100 98 98 98 95 Mean Dry Weight (mag)
Test Solutions (replicate number)______
(% Effluent) ] 2 [ 3 [ 4 Mean
- Control 0.616 0.492 0.528 0.579 0.554 10.7% 0.597 0.612 0.632 0.551 0.598 21.4% 0.618 0.538 0.559 0.644 0.590 42.8% 0.660 0.609 0.633 0.547 0.612 85.6% 0.617 0.624 0.588 0.633 0.616 100.0% 0.693 0.318 0.430 0.606 0.512 Intake 0.509 0.557 0.737 0.461 0.566 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: N__A_
Lower Limit: NA________ _______
- TUa = 100/LC5 0 : TUc =100/IC25 Page 5 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20 -27. 2015 using effluent from Outfall 101.
Percent Surviving Test (time interval used -days)
Solutions
(% Effluent) 1 2 3 4 5 67 Control ioo 100 100 100 10o 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 TestSoluionsReproduction (#young/female/7 days)
Test Solutions~Data
(% Effluent) 1if 2 J 3 [ 41.5_16 ]
(replicate number)__
7 8 r _____
9 ]. loMean Control 30 29 29 30 30 31 31 31 32 28 30.1 10.7% 33 31 30 30 30 32 30 32 35 32 31.5 21.4% 33 32 34 31 35 31 33 34 32 32 32.7 42.8% 32 36 32 34 34 33 33 34 31 33 33.2 85.6% 33 38 37 32 35 37 33 33 33 36 34.7 100.0% 36 34 39 38 32 39 35 36 37 36 36.2 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA _______________
- TUa = 100/LC5 0 : TUc =100/IC25 Page 6 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20 - 27. 2015 using water from Intake
~Percent Surviving Test ____ time interval
(___ used -_days)____ ____
Solutions 1 1
(%Efun) 112 3[ 4 516 7 Control 100 100 100 100 100 '100 100
- Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Data (replicate number)
(%Effluent) 1 2 3 I4 I 5 6 [7 I 8 9 10 I[Mean Conrol 30 2830 29 29 32 29 33 32 3.0k 30.2 Intake 135 34 34 33 36 34 31 32 31 32 33.2 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:.
Upper Limit: NA
- Ta=Lower Limit: N__A __________________
- Ta=100/LC 5 0 : TUc = 100/IC 25 Page 7 of 98
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic!/7-day Toxicity Test.
- (Genus species) (Type / Duration)
Conducted October 20 -27,.2015 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions interval used -_days)____
(____time
(%Effluent) 1 ] 2 ... 3 [ 4..] 5 ... J 6 7 .
Control ioo 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8%. 100 100 100 100 100 100 100 85.6% 100. 100 100 100. 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 TestSoluionsMean Dry Weight (mg) number)
(%Effluent) 1
______ ________(replicate 2_ ] 3 _4. . Mean Control 0.593 0.600 0.618 0.512 0.581 10.7% 0.542 0.695 0.566 0.558 0.590 21.4% 0.655 0.573 0.597 0.599* 0.606 42.8% 0.647 0.617 0.494 0.670 0.607 85.6% 0.559 0.614 0.568 0.623 0.591 100.0% 0.613 0.609 0.677 0.573 0.618 Intake 0.603 0.531 0.547 0.591 0.568
- IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: N__A Lower Limit: N__A
- TUa =100/LC5 0 : TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) ___
Species__ Date J Time [Duration Toxicant Results (IC25 )
Pimephalespromelas October 20 - 27, 2015 1130 7-days KC1 0.72 g/L Ceriodaphniadubia October 06 - 13, 2015 1030 7-days NaCI 1.06 g/L Page 8 of 98
Na PHXSICAL/CHEMICAL
SUMMARY
Water Chernistry Mean Values and Ranges forPimephalespromelasand Ceriodaphniadubia Tests, Non-treated SequoyahNuclear Plant (SQN) Out1*1 101 Co performed October 20-27, 2015.
0 Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual 0
____ Initial Final Initial Final Initial Final (pnmhos/cm) (mg/L CaCO3 ) (zng/L CaCO3 ) Chlorine (mg/L)
(D Cotl 24.8 24.5 7.7 7.6 8.02 7.81 317 63 88 24.7 - 24.9 24.3 - 24.8 7.5 - 7.9 7.0 - 7.8 7.95 - 8.13 7.66 - 7.94 302 - 335 62 - 64 88 - 88 1.% 24.9 24.5 .7.8 7.5 8.02 7.79 305 24.7 - 25.0 24.4 - 24.6 7.5 - 8.0 6.5 - 7.9 7.97 - 8.14 7.61 - 7.94 291 - 318 _ _____________
216% 24.9 24.5 7.8 7.5 8.03 7.79 288 24.7 - 25.0 24.2 - 24.6 7.6 - 8.0 6.2 - 7.9 7.98 - 8.15 7.58 - 7.94 280 - 296 _ ____ ______
4.% 24.9 24.5 7.8 .7.5 8.03 7.78 258 24.7 - 25.0 24.4 - 24.7 7.6 - 8.0 6.0 - 7.9 7.98 - 8.13 7.55 - 7.92 251 - 267 ______
86.4% 24.9 24.6 7.8 7.5 8.02 7.78 201
- g.24.8 - 25.1 24.4 - 24.7 7.6 - 8.0 6.0 - 7.9 7.95"- 8.06I 7.52 - 7.92 191 - 211 _ ____ _______________
10.% 25.0 24.6 7.9 7.6 8.00 7.79 181 72 77 < 0.10 24.8 - 25.1 24.4 - 24.7 7.6 - 8.0 6.6 - 8.0 7.89 - 8.07 7.55 - 7.94 170 - 189 69 - 73 69 - 90 <0.10 - <0.10 Inae 24.9 24.5 7.8 7.6 8.02 7.81 181 70 68 <0.10
_____24.7 - 25.2 24.4 - 24.6 7.6 - 8.0 6.8 - 7.9 7.93 - 8.09 7.62 - 7.97 172 - 191 67 - 71 61 - 73 <0.10 - <0.10 Coti 24.8 25.0 7.7 7.9 8.02 7.98 317 63 88 24.7 - 24.9 24.5 - 25.2 7.5 - 7.9 7.7 - 7.9 7.95 - 8.13 7.92 - 8.07 302 - 335 62 - 64 88 - 88 _________
1.% 24.9 24.9 7.8 7.8 8.02 7.99 305 24.7 - 25.0 24.5 - 25.2 7.5 - 8.0 7.8 - 7.9 7.97 - 8.14 7.92 - 8.07 291 - 318 21.6% 24.9 25.0 7.8 7.9 8.03 7.99 288 24.7 - 25.0 24.6 - 25.3 7.5 - 8.0 7.8 - 7.9 7.98 - 8.15 7.92 - 8.06 280 - 296 _ ____
4".2% 24.9 24.9 7.8 7.9 8.03 8.00 258 24.7 - 25.0 24.5 - 25.2 7.6 - 8.0 7.8 - 8.0 7.98 - 8.13 7.93 - 8.09 251 - 267 _ ____
24.9 25.0 7.8 7.9 8.02 8.01 201 86.4%/
24.7 - 25.0 24.8 - 25.1 7.6 - 8.0 7.8 - 8.0 7.95 - 8.06 7.94 - 8.09 191 - 211 _____ _________
10.% 25.0 25.0 7.9 7.8 8.00 8.01 181 72 77 <0.10 24.7 - 25.1 24.9 - 25.2 7.6 - 8.0 7.6 - 7.9 7.89 - 8.07 7.94 - 8.09 170 - 189 69 - 73 69 - 90 <0.10 - <0.10 Inae 24.9 24.9 7.8 7.8 8.02 8.03 181 70 68 <0.10
_____ _____24.7 - 25.0 24.7 - 25.0 7.6 - 8.0 7.7 - 8.0 7.93 - 8.09 7.96 - 8.14 172 - 191 67 - 71 61 - 73 <0.10 - <0.10 Overall temperature ("C) Average Minimum Maximum Pimephalespromelas ,24.7 24.2 25.2 Ceriodaphniadubia 24.9 24.5 25.3
In PHYSICAI/CIIEMICAL
SUMMARY
Water Chemistry Mean Vabies and Ranges for Pimephalespromelas Test, UV-treated S equoyahi Nuclear Plant (SQN) Outfl~sl 101 perforined October"20-27, 2015.
0 0 Test Sample ID) Temperature (°C) Dissolved Oxygen (nag/L) pH (S.U.) Conductance Alkalinity Hardness *Total Residual
-Og ID _____Initial Final Initial Final Initial Final (pumhos/cm) (mg/L CaCO3 ) (mg/L CaCO 3) Chlorine (mg/L)
Co Cotl 24.9 .24.5 7.8 7.5 8.02 7.81 316 63 86 24.8 - 24.9 24.4 - 24.7 7.7 - 7.9 6.7 - 7.9 7.95 - 8.12 7.64 - 7.95 302 - 328 63 - 63 86 - 86 _______
108% 24.9 24.5 7.8 7.5 8.02 7.80 303 24.8 - 25.0 24.3 - 24.7 7.8 - 7.9 6.7 - 7.9 7.96 - 8.13 7.60 - 7.93 291 - 312 ______
26% 25.0 24.6 7.9 7.6 8.03 7.81 288
,24.9 - 25.1 24.3 -'24.7 7.8 - 7.9 6.7 - 7.9 7.96 - 8.14 7.61 - 7.93 278 - 298 ______
42%
_____24.9 25.0
- 25.1 24.224.5
- 24.7 7.8 7.9
- 7.9 .7.5 6.0 - 7.9 7.97 8.03.
- 8.15 7.53 7.79
- 7.92 251 260
- 275 _____ ______
86.4% 25.0 24.5 7.9 7.4 8.04 7.78 204 86"4___ 24.9 - 25.1 24.4 - 24.7 7.8 - 8.0 6.0 - 7.9 7.98 - 8.15 7.54 - 7.95 197 - 213 _______ ______
10.% 25.1 24.4 7.9 7.6 8.04 7.79 .184 72 73 < 0.10
_____25.0 - 25.3 24.3 - 24.7 7.8 - 8.0 6.8 - 7.9 7.97 - 8.16 7.53 - 7.96 174 - 193 71 - 73 69 - 82 <0.10 - <0.10 Inae 25.1 24.5 7.9 7.6 8.04 7.86 182 71 65 < 0.10
______ ____25.0 - 25.2 24.4 - 24.6 7.8 - 8.0 6.8 - 7.9 7.97 - 8.17 7.71 - 7.97 174 - 194 69 - 73 61 - 69 <0.10 - <0.10
- Note: Total residual chlorine was performed on non-treated.Outfall 101 and Intake samples.
Overall temperature (*C) Average Minimum Maximum Pimephalespromelas 24.8 24.2 . 25.3
SUMMARY
/ CONCLUSIONS Exposures to samples collected October 18- -23, 2015 from Outfall 101 resulted in no toxic effects to daphnids. Minnow survival was significantly reduced in minnows exposed to the full-strength effluent. Pathogenic growth was also observed in the gill area of minnows in the full-strength effluent.
The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids and minnows to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference may have resulted in the minnow mortality observed in the full-strength effluent.
Page 11 of 98
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimnephalespromnelas Tests were conducted according to EPA-821I-R-02-0 13 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-82 l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimeyhales promnelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphniadubia None Page 12 of 98
PHYSICAL AND CHEMICAL METHODS*
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B-2000.
- 5. The pH was measured by SM 4500-H+ B-2000.
- 6. Conductance was measured by SM 2510 13-1997.
- 7. Alkalinity was measured by SM 2320 B-1997.
- 8. Total hardness was measured by SM 2340 C-1997.
- 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaCI.
Sodium Chloride (NaC1 crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 98
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater. 22 "dEdition, 201l2.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 98
Sequoyah Nuclear Plant Bijomonitoring October 20 - 27, 2015 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 98
Table B-I1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hl-150M Hypochlorite mg/L mg/L mg/L mg/L mgiL mg/ 73551 mgiL mg/L TRC Phosphate Copolymer DMAD Azole Quat mgiL Quat TRC ____ _ _ _ _ _ ____EOiPO 02/06/2005 - <0.0042 0.028 0.010 - --
02/07/2005 , <0.0116 0.028 0.010 - - - 0.007 -
02/08/2005 , <0.0080 0.028 0.010 -....
02/09/2005 , 0.0199 0.028 0.010 - --
02/10/2005 - <0.0042 0.028 0.010 ----
02/11/2005 -0.0155 0.028 0.010 - - 0.007 06/05/2005 -0.0063 .... --
06/06/2005 0.0043--- - - - 0.037 06/07/2005 0.0103--- - - - 0.037 06/08/2005 - 0.0295-- - - - 0.037 06/09/2005 , 0.0129 - - ---
06/10/2005 - 0.0184 - ----
07/17/2005 - 0.0109 0.026 0.009 -- -
07/18/2005 - 0.0150 0.026 0.009-- - - 0.036 07/19/2005 - 0.0163 0.026 0.009-- - - 0.036 07/20/2005 -0.0209 0.026 0.009 - -- 0.014 0.036 07/21/2005 0.0242 0.026 0.009 - - --
07/22/2005 -0.0238 0.054 0.018 - - - 0.014-10/30/2005 - 0.0068 - - -- -
10/31/2005 - 0.0112 - - ----
11/01/2005 - 0.0104 --- - - 0.035 11/02/2005 0.0104-- - - - 0.036 11/03/2005 - 0.0117 - -- - - 0.036 11/04/2005 - 0.0165 - - - 0.035 11/14/2005 - 0.0274 - - - I 11/15/2005 - 0.0256 .....--
11/16/2005 - 0.0234 .... --
11/17/2005 - 0.0231 - - - -,
11il18/2005 - 0.0200 - -- - --
11/19/2005 0.01 16 - - - --
Page 16 of 98
Table B-i. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbronm PCL-222 PCL-401 CL-363 ... Cuprostat-PF H-130M Nalco H'150M MSW Hypochlorite mgiL mg/L mg/L mg/ mg/L mg/b 73551 mg/b 101 mg/b TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC _______ ____EOiPO Phosphate 11/12/2006 - 0.0055 ......... -
11/13/2006 - 0.0068 - , - - - 0.037-11/14/2006 - 0.0143 - , - - - 0.037-11/15/2006 - 0.0068 - - - - - 0.037 11 /16/2006 - 0.0267 - -- - - 0.037 11/17/2006 - 0.0222 ----- --
11/26/2006 - 0.0188 - - --- --
11/27/2006 - 0.0138 - - ------
11/28/2006 -0.0120 -------
11/29/2006 -0.0288------ -
11/30/2006 -0.0376 -------
12/01/2006 -0.0187 -------
05/28/07 -------- 0.015 05/29/07 ------- 0.036 0.015 05/30/07 -0.0084 -- -- 0.017 0.036 0.015 05/31/07 -0.0103 ----- 0.036 0.015 06/01/07 -0.0164 --- - 0.017 0.036 0.015 06/02/07 -0.0305 --- -- 0.015 12/02/07 -0.0241-- -----
12/03/07 -0.0128 -------
12/04/07 -0.0238- ------
12/05/07- 0.0158 ------ -
12/06/07 -0.0162--------
12/07/07 -0.0175- -----
04/13/08 -0.0039--------
04/14/08 0.01t24 - ------
04/15/08 0.0229 - -------
04/16/08- 0.0143 - - -- ---
04/17/08 0.0120 - - ---- -
04/18/08- 0.0149 - ----- -
10/26/08 -0.0260--------
10/27/08 -0.0151 ----- 0.017 --
10/28/08 - 0.0172 ----- - 0.041-10/29/08 - 0.0154 --- - - 0.018 0.041 0.030 10/30/08 ......- -- 0.041 0.030 10/31/08 - 0.0086 --- - - - 0.041 0.030 Page 17 of 98
Table B-i1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL- PCL-401I CL-363 Cuprostat H-130M Nalco iSpectrus H-i150M MSW Hypochiorite mg/L 222 mg/L mg/b -PF mgiL mg/b 73551 CT1300 mg/b 101 mg/ TRC mg/b Copolymer DMAD Azole Quat mg/L img/ Quat mg/b TRC Phosph EO/PO Quat Phosphate 02/08/09 ... ... 0.0197 - - - - 0.017--
02/09/09 - 0.0237 , - - 0.0 17 ---
02/10/09 ... - 0.0104 - - - - 0.021 . . . ,
02/1 1/09 -0.0155 -- -- 0.017 --
02/12/09 ' 0.0106 - --- - 0.017 --
02/13/09 - - - - ----
05/10/09 - 0.0129 - -- - --
05/t11/09 - 0.0415 - ----- - 0.0446 05/12/09 - 0.0053 - - --- - 0.0396 05/13/09 . 0.0049 - ----- - 0.0396-05/14/09 - <0.0141 - ----- - 0.0397 05/15/09 - <0.0160 ....- --
11/15/09 - 0.025 ------ --
11/16/09 - 0.0152 --------
11/17/09 - 0.0255 ---- ----
11/18/09 - 0.0306 ---- ----
11/19/09 - 0.0204 --------
11/20/09 - 0.0093 -- ------
05/09/10 - 0.0192 - - ----- -
05/10/10 - 0.0055 - - -- ----
05/11/10 - 0.0100 - ---- - 0.039 -
05/12/10 - 0.0171 - ---- - 0.039 --
05/13/10 - 0.0041 - --- - 0.039 --
05/14/10 - 0.0099 - --- - 0.039 --
Page 18 of 98
Table B-i. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbron PCL- PCL-401 CL,363 Cuprostat H-130M Nalco Spectrus H-I150M MSW Floguard Hypo- mgiL 222 mgiL mg/b -PF mg/b mg/b 73551 CT1300 mgiL 101 MS6236 chlorite TRC mg/ Copoly- DMAD Azole Quat mg/L mg/b Quat mg/b mgiL mg/b Phos- mer EO/PO Quat Phos- Phosphate TRC phate ____________phate ____
10/31/10 - - - ..... -- ,
11/01/10 - 0.0122 - - -- -----
11/02/10 - 0.0112 - ....- --
11/03/10 - 0.0163 - - --------
11/04/10 - 0.0107 --- ------
11/05/10 - 0.0132 ---------
05/01/2011 ....-- ---
05/02/2011 - ------ - 0.04 ---
05/03/2011 - - ---- - 0.04 ---
05/04/2011 - 0.0155 ----- - 0.04 - -
05/05/20 11 - 0.0 179 - -- - 0.04 ---
05/06/2011 - 0.0089 - - -- -----
11/06/2011 - 0.0168 - - -- -----
11/07/2011 - 0.0225 - - --- ---
11/08/2011 - 0.0141 ....- --
11/09/2011 - 0.0239 ....- --
11/10/2011 - 0.0242 - ---------
11/11/2011 - 0.0231 - ---------
05/06/20 12 -....- ---
05/07/20 12 -....- ---
05/08/2012 -..... -- 0.041 ---
05/09/2012 - 0.0145 ----- - 0.041 ---
05/10/201!2 - 0.0298 ----- - 0.041 ---
05/11/2012 - 0.0174 - --- -----
08/12/2012 ...... -- -- 0.029 08/13/20 12 - 0.0256 - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 - - - --- 0.037 - - 0.029 08/15/2012 - 0.0279 -- -- 0.028 -- - 0.029 08/16/2012 - 0.0076 -------- - 0.029 08/17/2012 - 0.0446 -------- - 0.032 05/12/2013 - 0.0099 ....- --
05/13/2013 -.... -- 0.064 05/14/2013 - 0.0091 -- - - 0.039 -- - 0.064 05/15/2013 - 0.0096 , - 0.039 -- - 0.064 05/16/2013 - 0.0229 - ------- - 0.032 05/17/2013 - 0.0063 - - ----- 0.032 09/15/2013 ....-- --- 0.03 09/16/2013 - 0.0072 - - -- - 0.0379 - - 0.03 09/17/2013i - 0.0107 -- - - 0.036 0.0379 - - 0.03 09/18/2013 - 0.0217 ----- 0.036 0.0379 -- 0.03 09/19/2013 - 0.0172 -- ----- - 0.03 09/20/2013 - 0.0173 ---- --- 0.03 Page 19 of 98
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologicaily Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H,130M Nalco Spectrus H-150M MSW Floguard Hypo mg/L 222 mg/L mg/ -PF mg/L mg/ 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mgiL mg/ Quat mgt mg/L mg/ Phos- mer EO/PO Quat Phos- Phosphate
_____ TRC ____ hat ___e____ ____phate ____
05/04/2014 0.0118 - - --- ,
05/05/2014 - 0.01 12 - - - - --- --
05/06/20 14 - 0.0096 - - , --
05/07/2014 - 0.0164 ....- --
05/08/2014 0.0235 - - -------
05/09/20 14 0.0110 - - ------ -
09/07/20 14 ....-- ---
09/08/20 14 --- - - -- 0.04 - --
09/09/2014 -- --- - - 0.04 - -
09/10/2014 --- ---- - 0.04 -- -
09/11/2014 - 0.0070 ....- --
09/12/2014 - 0.0074 ....- --
08/09/2015-08/10/2015 0.0195 0.03 08/11/2015 0.0275 0.03 08/12/2015 0.0213 -0.03 08/13/2015 0.0192 -0.03 08/14/2015 0.0182 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 0.0174 10/23/2015 0.0156 Page 20 of 98
Sequoyah Nuclear Plant Biomonitoring October 20 - 27, 2015 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 21 of 98
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1_of 1
- ient: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
l~oject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx LUPS Bus Client
-0. Number: N/A Asheville, NC Other ~
- cility Sampled: Sequoyah NP 28801 General Commen~ 5:
INPDES Number: TN0026450 Phone: 828-350-9364 Fax: 828-350-9368 U- U - U U Xbr~~. ,W~44% C.~4t~ CbIIeCAV~4'14 14+ ~.
Flow Collection Date/Time Container Number &
Volume (MGD) Rain Event?
(Mark as Appropriate)
- ,P~V~M 1~,
borat~zyt~
I 11111 V'-~A ~T~'-~
Yes If Yes, No Trace ETSLog. ArrivalTeizip. By. ~Tim~ App~ar~
Inches $umbcr (CC) I End
__ 1__ __
I'=!
L ~L.'gaI) 4:
/ 799. zr-SQN-TNT-TOX Comp
,__ I 7/5
,"-/'q-I5
~ *t4/*'4,.,i^.
(. gal) NA Ij:,** ' .
__________________________Sample Custody - Fill In From Top Down _______________________.__
Relinquished By (Signature): Date/Time Received By (Signature): G~* .t -
- a*~*XDate/Timel*tk. '~.
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnighit on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Whole Effluent Sample Receipt Log Page 4i¶*
f7~i~
w
~II" Date Tim Received Received "*Sample Project SampleSapenmandscllo
- Sample temperaure performed using Samlple Receiving~Thenmomnter2 State Comments SN 130580151 (D
10-19-15 1225 1.Sumner VA Courier 1.8* 1.9 10958 151019 .01 TVA -e uoyh Nudear Plant -101 TN 10-19-15 ... 1225 .......
LSumner VA CourIer 1.6 10958 "151019 .02 VA - Seuoyh Nudear Plant - INT TN 0
-em SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I
- hent: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client
.O. Number: N/A Asheville, NC Other seiy:.* l.. e 'e/*
~cility Sampled: Sequoyah NP 28801 General Comments:
Phone: 828-350-9364 I1thl Mz.v-*l,6 *,rt%U) s~4i, ,.,,',,,,o,..e.,,
O,,3 NPDES Number: TN0026450 Fax: 828-3 50-93 68
_______ _______ ____ ___ h - ------- --- -
Collection Date/Time Container FlowI Number & (MGD) Rain Event?
I Volume Yes (Mark as Appropriate)
If Yes, Inches No Trace tT~Log ~
tZor~tory~Jse Amv4 Temp.
c~Cy By ~Appear End
/0-ZO- /$~ ~o-ZI-l~5
~) L,~IoE*+
I I - -~ -- I I 1 ¶ T -.
SQN-1NT-TOX [Comp /O-~D ~ '0-74-Is- 1 (2.5 gal) NA I llI -ll I
£2 73'o
~ I i~Ip? O~
- - - - I I - I - I - .. .... ...... . I .... I -1.. "
Sample Custody - Fill In From Top Down Reli.nquished By (Signature): Date/Time Received By (Signature):
I 6~S
___________________________________________________________________ I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing Within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they' will not arrive until the following Monday.
Page T Whole Effluent Sample Receipt Log
'SniI teoeatr cefo, e usnvni Rec n lhro . e N 1... 8.1. 1 lime Received Received *Sample Project Sample i....
Date- 0 Received . Received by from Temp. ( C) number .number Sample name and dtescription State Comments
- ~- -I 10-21-15 0950 K.Keenan Fed - Ex 1.8 10968 "I151021 .01 ALCOA - 005 .. _____N__ ........ ....... ___
U' 10-21-15 09,50 K.Keenan Fed - Ex. 1.8 10968 151021 .02 ALCOA -012 _________ _NC________
10-21-15 0950 K.Keenan Fed - Ex 2,6 10969 151021 .03 SmithfieldPacking _____NC___ .....
0 10-21-15 0950 K.Keenan Fed - Ex 1,8 10970 151021 .04 ALCOA - 013 ____________' NC________
10-21-15 0950 K(.Keenan Fed - Ex 2.0 10971" 151021 .05 Dswberry - Regen - Grab ,_____"'__ ____________"_
10-21-15 0950 K.Keenan Fed - Ex .. ,2.0 '10971 151021 .06 Dewberry- Effluent.- Grab) ________ NC ___________
10-21-15 0950 K.Keenan Fed - Ex 2.6 10972 151021 .07 Beaufort - Glenda Drive WTP ___________ NC _________
10-21-15 0950 K.Keenan Fed - Ex 2.6 10973 151021 .08 Dixon WTP ________NC_____
10J-21-15 0950 K.Keenan Fed - Ex 4.2 10974 151021:.09 Dare County WTP ;N 10-21-15 0950 K.Keenan Fed - Ex 4.2 10975 15102,1..10 RWS. ________ _NC________
10-21-15 0950 K.Keenan Fed - Ex 4.2 10976 151021 .1 1 Skyco W*TP ___________ NC _________
10-21-15 0950 " K.Keenan Fed - Ex 4.2 10977 151021 .12 Stumpyv Point WTP ____ ___________
10-21-15 -0950 K.Keenan Fed - Ex 1.2 10978 15102,1..13 New Hanover WTP ______NC____
10-21-15 0950 K...K Keenan Fed - Ex 1.6 10979 - 151021 .14 Richland Township WTP _____NC__
10-21-15 1025 J. Sumner UPS 1.0 10980 151021 .15 Atlantic Be.ach WWTP ____N 10-21-15 1025 J. Sumner UPS 0.9 . 10981 15102.1, .16 Camden County.WTP _________ NC 10-21415 . 1230 J.Sumner W Courier TA .1.0/0.9 .10958 151021.,.17. TVA - Seq uoyah Nuclear Pla~nt* 101 TN J 10-21-15 .1230 J. Sumner
- TA Courier 1.6 10958 151021 .A8 TA - Sequ-_yah Nuclear Plant- Intak.e TN 10-21-15 1540 J. Sumner. TVA Courier 1.8 10982 151021 .19 TVA -JOF ME2C- Simulated Effluent TN 10-21-15 . 15,40 ,J. Sumner TWA Courier 2.0 10982 151021 .20 . VA-JOF ME2C- Upstream "TN SOP G4 - Exhibit G4.2, revision 0 1-03-12
SBIOMONITORING CHAIN OF CUSTODY RECORDPae1oI_
w (A~ient: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
rn-oject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client
=?O. Number..N/A Asheville, NC
.Other(seiY: 'i, b !;*,'[
- cility sampled: Sequoya1 NP 28801 G~eneral Comments:** -
%Ag, .%epS',*, 4'%"? i 6cr,tx.,,P'f o"/z--<
NPDES Numnber:. TN0026450 Phone: 828--350-9364 Fax: 828-350-9368
~dB:
p a ~- I-~ a n i i n m n n B1 I I Container Number & ~?2ItA4) Q44 1 ' I D~
Grab/Comp. Rain Event?
Sample Description Collection Date-/rIime Volume Laborate ry Use I
&~' c~. ~~o- %?
~--i~-~s .
Instructions: Clients should fiil in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample
- is36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page O Whole Effluent Sample Receipt Log
.5 *SampIetempe~um peiformed usingSanip!e ReceMncThermometer SN230550151 RI (a . Date
- Received lime Received Received by Received from
- Sample Temp. (0C
.Project number Sample number Smlnm S e..m n *fpimSaeCmet an dtsripinStt om e 0
10-23-15 0950 J,. Sumner Fed - Ex 1.3 10968 '151023 .01 ALCOA - 005 Nc ___________
R.J 10-23-15 0950) J. Sumner Fed - Ex 1.3 10968 151023 .02 ALCOA -012 NC._.N_________
0 10-23-15 0950 J.1.Sumner Fed - Ex 1.4 10969 151023 .03 Smithfield Packing iNC___________
-Ii *10-23-15
- 1250 J. Sumner WVA Courier 2.4, 1.4 10958 151023 .04 TVA - Seq uoyah Nuclea~r..Plant -101 .... TN ___________
(0 10-23-15 1250 J. Sumner TWA Courier " 0.8 10958 .151023 .05 VA - Sequoyah Nuclear Plant - Intake ' TN ___________
SOP G4 - Exhibit G4.2, revision 01-03-12
___ /
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-8231-R-02-013 Method 1000.0)
Species: Pimephalespromeias Client: Tennessee Valley Authority County: Hamilton Facility: Sequovah Nuclear Plant Outfall: 10__1 NPDES #: ,TN0026450 Project #: *cC*
Dilutionprepara~tioninformation:
Dilution prep (%) 10.7 21.4 42.8 I85.6 100
.~ Comments:
I Diluent volume (mL) 2232.5 1965 J1430 I360 j 0 _______________I
- Totalvolume (..L.)., 2500 2500 2500 2500 *2500 Test organism information: ...... Test information: __.........
Organism age: . o%* * *.7 t 0* Randomi zin g template: _____._*.
Date and times organisms 10-19-15 1600-1700 Incubator number and were born between: ______________shelf location: ____
Organism source: ATOX Batch Pp: 10-19-15 Artemia CHM number: CHM836 Drying Infonnatlonfor weight
______________________deterimiation:____
Transfer bowl information: pH = t . S.U.' Date!/Time..in oven: ].%t
_________Temperature.= ". 0C .. Initial oven temperature: * '...0 Average transfer volume: 0.1749 rL .. :Date / Time out of oven: .,t Final oven temperature: ,o*
._______ ___________Total drying time: 'Z.*. 6.
1)ailyfeeding andrenewal information:
Day Date Morning feeding A~flernoon feeding "" Test initiation, Sample numbers used MHtSW renewal, or batch
________ ____ termination used Time
,,____ Analyst Time Analyst ... Time Analyst Outfall 101 I ... Intakce,,_
0s l0-20-15 _______
- I,.OO *L.... %o$% ' .. .Stb*.O\
% r*
- t I l o-*
6 10-26-15 o 11 At I e15
'7 10-27-15 "
Controlinformiation: ... Acceptance criteria *Summary of test en~dpoints:
% Mortality: 0,* 20% 7-day LCso ? irt, Average weight per initial 1arvae: or NOEC __________
Average weight .persurinvin'g. larvae: .... o Ž0 .2,5mgfl~ar'ae .... .OEC . *.
ChV IC25 ________
SOP AT20 - Exhibit AT20.3, revision 11I-01-I4 28 of. 98
- * . . . .P a g e 2 of 6 Species: Phmephales.promelas Client: TVA / Sepnovah Nuclear Plant. Outfall 101.,NON-trea~ted Date: 10-20-15 Survival and Growth Data ayCONTROL 17%21.4%-
______AI B C D E F G H I J K L 10 10 10 10 10 10. t0 "10 10 10 10 10
.. 4* 0 t D t O t D r O t 5 *t Ot O 0* 0* Ot 6 b e
- I b P * *
- I 6
- A= Pan weight (mag).
Tray color code: *".
Date: OQ-fl'- ,
B'= Pan + Larvae weight (rag)
C 'Larvae weight (mag)=fB - A ..
HAndalyusted *S li)t d~~' ~ ~ ~ A Analy.it:
Weight per initial nnnmber of larvae (mg)
= C / Initial number of larvae V I.
Hand calculated.
Analyst: . .
a'
~j~a
'0 .4. '0 C,
_.._I ..-
Averg egt ofper initial number Percent reduction from coatol (%) f , ' 4
- .- IS ~01 -. ~ *-
larvae (mg) I J Comment codes: c = clear, decanted d = dead, fg = Iun.gus, k =killed, m =missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = and returned, w ffwounded.
Pa e 29 of 98 SOP AT20 - Exhibit AT20.3, revision. 11-01-14
Page 3 Of 6 Species:. Pimephalespromelas Client: TVA / Sepuoyah Nuclear Plant, Outfall 101, NON-treated Date: 10-20-15
________________ ____________Surrival and Growth Data "_______
Dy42.8% 85.6% _ 100%
10 10 10 10 10 10 10 10 10 10 10 10 4 ~10Ib 16 / lO I'D 1O LO . 1Q 10 ,*,
40 A-= Pan weight (rag) .. .
Tray color code:: t Date: 0o -2*__-15 B= Pan + Larvae weight (rag) 591 '
Date: ______ -______"
C =Larvae weight (rag) =B - A Hand calculated. ~~~'A% ~ ~ A'U ~ ~ ~~
Analyst: t . ..
Weight per initial number of larvae (mng)
= C/IInitial number of larvae Analyst:. * " "
- J Average weight per Percent reduction initial nunmber of fromncontrol (%) 0 * *- -- 4 L0,
- i A o *,0*
- 0* ,*ol larvae (rag)_______ _____________ _ _____
Comment codes: c =clear, d = dead,.fg = fungus, k = killed, m = missing, sk =sick, sm = unusually small,
-lg= unusually large, d&r = decanted and returned, w = wounded.
Comments: -* P,*' ea &¢. Ca~*t2*%*.Jt.R.. ¢r._ A.e 0* l f I.d.p.nd.,t
~
1k- SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 30 of 98
Page 4 of 6 Species: Pimevhale$oromelas Client: TVA / SeiuoyahNuclear Plant, Outfal ,1,0,1. NON-treated Date: 1t)-20-15 Day 100% Intakff*me 0 0 0 1 10 2 10 0(0 O 6
A = Pan weight (mag)
Tray color code:: ...
Analyst: ... ' * (
Date: __ *_____-____
B = Pan + Larvajr weight (ing) - -
- Analyst: 'j ~ ~ tL Date: i*-&g-i5- . [*o C = Larvae~weight (mag) = B- A Hand calculated.. "*
Analyst:
Weight per initial number of larvae (mug)
C /Initial number of larvae
- Hand calculated. **
Analyst: . .* *o Avrg egtper Percent reduction initial number of [ from control (%) *
- 2,,o' larvae m ________________1________________
Comment codes: c --clear, d = dead, fg = fungus, k = killed, m = missing, sk =sick, sni- unusually small, Ig =unusually large, d&-r - decanted and returned, w = wounded.
Commeents:
- Page 31 of 98 SOP AT20 - Exhibit AT20.3, revision 11-01-14
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015
.5O .PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Ic Project numbern 10958 C~eeprlteal(tt) ReIwen ~lettull hu lrel" Flit nmetedt* A=Pan wdkt(m) B=Nal+Lm'en tJmnnawetlt(30), Wetshf~lL,ndele MznwelJel Caeleet~ri~vzt'tmltna Weg~lkt/ fJndttcmber Melan annex Meuxwghtf In~tfllafl Cedthtextef Peeseutndxleefmi A___ 10 "10 15.61 21.77 6.16 0.61'60.6 Control B 10 tO 16.01 20.93 . 4.92 0.492 0.5.54 9.9 0.492 100.0 0.554 9.9 Net applicatble C 10 10 1627 21.65 5.28 0.528 0.528 D 1_1_1.2_*2208 ,,5.9 _057 ,,,0.579 _____ ______ _____ _ ________
E . 0 10 16.16 22.13 5.97 0.597 0.597 G 10 10 15.77 22.09 6.32 0.632 056.80.612 10.7_ ______G10 _______
15.77_ __ 2.09___ 6.32____ 0.632 0.598 5.0.632 100.0 0.598 5.8 -8.0 H 10 10 16.36 21.87 ,5.51 0.55r - , _____ ________ 0.551 _____ ______
____ 10_____ ____0___ 15.08____ 23.26____ 6.8 060.618 214 01 5. 06 .8058050S40.3538 100.0 0,590 8.4 -6.5 1
!0 10 . 15.26 20.64 5.5890538 0590 .40.559 L IO 010 16.22 22.66 6.44 d.644 _______0.644.____________ ______
- 4. N 19 .22.36.9.77069920.609 97.5 0.612 7.9 -10.6 10 90 16.29 22.38 6.0930677 0.299 0.633 P . 10 10 10.06 21.53 5.47 0.547 0.547 ____________________
_a...Q 10 10 15.89 22.06 6.17 0,617 0.617 8.5.691 1K 10 10 15.91 22.15 6.24. 0.624 0.616 3.2 0.624 100.0 0.616 3.2 -11.2 S 10 10 16.12 22.00 5.88 0.588 0.5*88
_______ T 10 10 ". 16.81 23.14 6.33 0,633 _______ _______ 0.633 _ __ _____ ______ _______
U 10 9 . 16.47 23.41) 6.93 0.770 0,693 100% V 10 6 16.11 19.29 3.18 0.530 0.673 15.3 0.430 75.0 0.512 33.1 7.6 W 10 6 16.61 20.91 4.30 0.717 .0"
________ X 10 9 15.71 21.77 6.06. 0.673 ________________ 0.606 ____ _____________________
- 10) LU t0.35 21.44 3'.01* U,)O9 7; 10 10 15.66 5.57 7 0.595 16.6 95.0* 0.566 21.3 -2.2 1410%ILntake 0.737 0.461 MSD = Minimum Sirtlni a-t Difference Dunoeff.'s M5D vanne: 0.0721 PM48D- Ferrcri Mi~nium Signifler.ot Difference 03.0 percent differenoe betweenthe eoab'ol wtdtirealmoent P1450 isan mennoue of test precision. The 1'MSIDis the mninismum significant mnawhole effluent toxicity lest that car be declered atatlstica11y Dunneti's 6660 value: 0.1286 Lower £1451 hatred determoined by USEPA (10th percentile) = 12%.
£6M80: 23.3 Upper PMSD bossnd determined by US8PA (90th perrerstile) = 10%.
Lower and. upper PMS50 b*oundswere determined from the lOtliand 901kperetle, respectivey, of P1430 data hoot EPAb WEI"I-ttulehoratosy Voribility Study (USSPA, 2001a; USE.PA, 200thb).
USEPA. 2001o, 2001b. Final Report: lnterlaborutosy Variability Stadyof EPA Solmrt-trm Chroaloond Ac~ute Whole Effluent Toxicity Teat Methods. Volumes 1 cod6 2-Appendix. EFA-821-B-01-004 and EPA-821-B~0-00.(X5. US Environmental Prcieclion A~eec, Cincinnat, OH.
Flu: .sqnl 01_i02Ol4Sdata.sxta Entered br: .1.S*.no Reviewed by:.!
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015.
a statistical Analyses
~ EnvJronm~ntaITe~t1nfl So1ulIor~.
Inc.
Larval Fish Growth and Survival Test-7 Day Survival Start Date: 1012012015 Test ID: PpFRCR Sample ID: TIVA /SQN Outfall1101 End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sol.- Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2015 Protocol: FVWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000.
- 10.7 1.0000 1.0000 1.0000 1.0000
- 21.4 1.0000 1.0000 1.0000 1.0000 42.8 1.0000 0.9000 1.0000 1.0000 85.6 1.0000 1.0000 1.0000 1.0000
- 100 0.9000 0.6000 0,6000 0.9000 Intake 1.0000 1.0000 1.0000 0.8000 Transform: Arcein Square Root .Rank 1.Tailed Conc-% Mean N-Mean Mean -Min Max , CV% N Sum Critical D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 10.7 1.0000 1.0000 1.4120 1.4120 1..4120 0.000 4 18.00 10.00 21.4 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 *4 18.00 10.00 42.8 0.9750 0.9750 1.3713 1.2490 1.4-120 5.942 4 16.00 10.00 85.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 "4 18.00 10.00
- 100 0.7500 0.7500 1.0676 0.6861 1,2490 19.630 4 10.00 10.00 Intake 0.9500 0.9500 1.3358 1.1071 1.4120 11.411 4 Auxiliary Tests Statistic Critical . Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.73225 0.884 -0.1438 2.5003 Equality of variance cannot be confirmed Hypothesis Test (1-,tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 85.6 100 92.5203 1.16822 Treatments vs D-Control Ds-epne DoseRespnsePlot 0.9 0.8 0.7
"*0.6 W 0.5 i* 0.4 0.3 0.2 0.1 File: sqnl OI_1020l5data.xlex Bdtered by: I. Sumner Reviewed by:_ _
33 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101
.Non-treated 0
October 20-27, 2015 0
- Statistical Analyses*
© Larval FishI Growth arid Survival Test-7 Day' Growth Start Date: 1012012015 Test ID: PpFRCR Sample ID: TVA/SQN Outfall 101 End Date: 10/27i2015S Lab ID: ETS-Envlr. Testing Sol. Sample ~Type: DMR-Dlscharge Monitoring Report Sample Date: October 2015 Protocol: FWCHR-EPA-821-R-02-013 Test Specles: PP-Pimephiales promeles Comments: Non-treated Conc-% 1 2 3 4 0-Control 0.6160 0.4920 0.6280 0.5790 10.7 0.5970 0.6120 0.6320 0.5510 21,4 0.6180 0.6380 0.6590 0.6440 42.8 0.6500 0.6090 0.6330 0.5470 86.6 0.6170 0.6240 0.5880 0.6330 100 0.6930 0.3180 0.4300 0.6080 Intake 0.5090 0.5570 0.7370 0.4610 Transform: Untransfo~rmed 1-Tailed Isotonic Cone-% rMean N-Mean Mean Mmn Max CV% N t-Stat Critical MSD Mean N-Mean D-Control. 0.5538 1.0000 0.5538 0.4920 0.6160' 9.885 4 0.5939 1.0000 10.7 0.5980 1.0799 0.6980 0.6510 0.6320 5.762 4 -1.446 2.360 0.0721 0.5939 1.0000 21.4 "0.5898 1.0850 0.5696 0.5380 0.6440 8.401 4 -1.178 2.360 0.0721 0.6939 1.0000 42.8 0.6123 1.1058 0.6123 0.6470 0.6600 7.878 4 -1.914 2.360 0.0721 0.6939 1.0000 856. 0.6155 1.1115" 0.8155 0.5860 0.6330 3.163 4 -2.020 2.360 0.0721 0.5939 1.0000 100 0.5118 0.9242 0.6115 0.3180 0.6930 33.077 4 0.5118 0.8617 Intake 0.5660 1.0221 0,5880 0.4810 0.7370 21.298 4 Auxlllary Teats Statistic
- critical Skew Kurt Shapiro-Wilk's Test Indicates normal distributIon (p > 0.01) 0.95853 0..868 -0.2242 -0.9437 Bartlett's Test indicates enua.l variances (p 0.58) 2.87245 13.2767 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChY TU MSDu MSDp 'MSB MSE F-Prob df Dunnett'sTest 85.6 >85.6 1.16822 0.07214 0.13027 0.00246.0.00187 0.31073 4,15 Treatments vs D-Control Linear Interpolation (200 Resamuptes)
Point %I SD 95% CL(ExoI Skew IC05 96.016 I101
>100 1.0:
IC20 >100 0.9, IC25 >100
>1*00 0.8, lC50_ >100 0,7 0.68 0.-
0.-
0.2 0.1:
0.8
-0.1
-0.2 0, 50 100 150 Dose %
Dose-Response Plot
- 0.51 0.4i 03.3 0.2 0.1 File: sqn]Ol_102015data.xlhx Enlered by: 1.Silt~er Page 34 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated October 20-27, 201.5 0
Statistical Analyses EtwlronmentalTestlng Solutloni. Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/20/2015 Test ID: PpFRCR Sample ID: "rVA / SQN Outfall 101 - Intake End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Pate: October 2015 Protocol: FW'CHR-EPA-.821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 .3. 4 D-Control 0.6160 0.4920 0.5280 0.5790 10.7 0.5970 0.6120 0.6320 0.5510 21.4 0.6180 0.5380 0.5590 0.6440 42.8 0.6500 0.6090 0.6330 0.5470 85.6 0.6170 0.6240 0.5880 0.6330 100 0.6930* 0.3180 0.4300 0.6060 .
Intake 0.5090 0.5570 0.7370 0.4610 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Mini Max CV% N t -Stat Critical MSD
- D-ControT 0.5538 1.0000 0.6538 0.4920 0.6160 9.885 4 1.0.7 0.5980 1.0799 0.5980 0.5510 0.6320- 6.762 4 21.4 0.5898 1.0650 0.5898 0.5380 0.6440 . 8.401 4 42.8 0.6123 1.1056 0.6123. 0.5470 0.6600 7.878 .4 85.6 0.6165 1.1115 0.6155 0.5880 0.6330 3.163 4 100 0.5118 0.9242 0.5118 0.3180 0,6930 33.077 4 Intake 0.5660 1.0221 0.5660 0.4610 0.7370 21.298. 4 -0.185 1.943 0.1285 Auxiliary Tests . Statistic Critical' Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) "0.93339 0.749 1.07115 1.28224 F-Test indicates equal variances (p = 0.23) 4.85006 47.4683
.Hypothesis Test (1-tail, 0.051 M$Du MSDp MSB MASE F-Prob df Homoscedastic t Test indicates no significant differences 0.12863 0.23229 0.0003 0.00876 0.85928 1, 6 Treatments vs D-Control Dose-Response Plot 0.7' 0.6.
1-tail, 0.05 level
(- 0.4. of significance
- 0.31 h,~p*~.ot R.~d.Wby Y.eij.iLJC..r.n:
File: sqnlOIWl2OlSdata.xlsx Entered by: .1.Sumner Reviewed by:
Page 35 of 98
'* ........ Page 5 of 6 Species: Pimephalespromelas Date: 10-20-15 Client: TVA'/Sepuovah Nuclear Plant. Outfall 101. NON-treated Daily Chemistry:*
- _Day Analyst
=*====TConcentration Parameter /
pH (S.U.) i .....
DO fmn/I' A. -,
Conductivity CONTROL (~unhosIcm) 301 NON-treated *Alkallnity
- (mg CaCO3/L) ________
- Hardness (mg CaCO3IL) _______
"1Temnerature (CC) .~ .- ~
i pH (s.u.) S~O2~ ~ -j 10.7%
DO (ma/LI Conductivity * .
L1~ .1
- ac~~
- Temrjerature (CC')
a - -
pH (s.u.) *,3II 77 17 ,.q
- U7.$# It DO(mu/L) - 7 21.4% Conductivity II
(~+/-mhos/em) 2YA
~ratur~0 c~ -iA.
S 42.8%
pH(S.U.)
DO(mafL')
Conductivity I ~.Q2- b~t 0a T1L (Mrnhos/cnl)
- Temnperature (°C)
S I. ~
nH (S.Ul
- ,.o'2.
-- 1:*
DO (mrJL) 85.6% ' Conductivity
- Temperature (°C)
- o~& -~-
Conductivity ~ <.0
(~cmhos/em) 100% (mc CaC0~/L) a
- Hardness (mc CaCO~dU
- TR chlorine (rng/L) do
- Temperature (°C) k~l a - -
oH (S.U.')
...... *- I " "-- 41,,
I ~A 7q~ H DO (ma./L) .0 lb I U Conductivity (unrhos/em) ~I-v4~, 11 10o% *Alkalinity
- ntake (nig CaCO3IL)
- Hnrdaess (rag CaC0~IL)
- TR chlorine (mag/i) 0.10 a * ,' C.
11ita Fna niia inl Initial Fia
.dpn*. peratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
/ ,kanity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for e~ach analysis and transcribed to this bench sheet.
(*":
8**.Total residual chlorine was performed on non-treate 0utih 101 anid Intake samples.
\ [A / **___" "SOP AT20 - Exhibit AT20.3, revision 11-01-14
. Page 36 of 98 """
........ Page 6 of 6 Species: Pime~halespromelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. NON-treated Date: 10-20-15 N
Ia a
II Day (Analyst identified for each day, performed pH, D.0. and conductivit measurements onl . '
Analyst F= I Concen-tration IParameter pH (S.U.)
DO (mg/L,) E.
Conductivity CONTROL (jxmhoslcm)
- NON- *Mkalinlty treated (mg CaCO3/L)
- Hardness (rag CaC0sJL)
- Temnnerature (0C) 'Lie.
PH (S.U.)
- 1o-~
DO (rg/L). 7 10.7% Conductivity
- Temuerature (CC) pH (s.u.)
0o (mg/L) 21.4.% Conductivity (umhos/cm)
- Temperature (°C)
-~ (OTT~.
DO (ma./LI 42.8%
I(unihosloml
- Tjnerture (00) niH (S.U.')
- DO (mg/LI 85.6% *Conductivity J '..
- Temperature (00)
DH (S.U.)
DO (mn/LI Cond~uctivity
- Alkalinity 1 100%
- Hardness I
- TR chlorine (rag/I.)
- Temneratnre (00) i'~4QQ II H(S.U. .
DO(me/L) ~7'P Conductivity
- Alkalinity 100%
Intake
- Hardness I(rag CaCOi/L)
- TR c~hlorine (mglL) 'C.
SH ~ ~- I
.............................- Initial II Final l Initial [ Final *'Initial Final Initial II Final "I..
ndp*Tperatures performed at the time of test initiation, renewal or termination by the-analyst-idlentfied in te Daily Renewal Inflo-r~mation table lo-cate d on P~age-i
[ hardness and total residual chlorine performned by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet ar:*~nty.
bky' * * - Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.*"
o*SOP
- AT20 - Exhibit AT20.3, revision 11-01-14
TVA / Sequoyah Nuclear Plant, Outfail 101 - Non-treated October 20-27, 2015 IEF Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemicai Analyses
.O Environmental Testing Solutions, Inc. Project number: 10958 Parameter Lz!L~ DaZ Da3 Da4 DeS Da6 Concentration lInidalIFinall I Initial Final Initial Final Initial Final Initial Filial Initial Final
.9.----- 802 7.66 8:13 792 8.08 7.76 pH (SU) I 5.001I '6 7.95 7.90 7.96 7.75 DO tmnn/L* 6 7.5 7.8 7.7 7.6 7.9 7.0 7.7 7.8 7.6 7.8 Codetiiy (wnhoslcmi) ___ 310 325 . 302 317 335 Control 63 64.
Alkalinity (mg/L CaCO 3) __
Hardness (mgF CaCO) L____ 88 88 -
C I 24.81 24.6 24.81 24, 3 24.8 24.6 24.7 24.4 24.9 24.8 24.8 24.4 24.9 8.02 24.6 7.77 H(SU) 802 794 802 776 797 785 799 768 800 7.61 814 790 Temperature 10.7% DOlniziL) (wnhoslcrn) 18 78 78 76 75 78 78 73 80 6.5 78 79 7.8 7.8 Conductivity _______ _______ _______ 310 2C.1 . 2'9 305 0 244 249 24.6 249 246 25.0 24.4
__________ Tcmnperature( C) 248 244 249 246 249 246 247 H(5U) 802 794 803 775 798 788 800 769 801 7.58 815 789 8.04 7.80 21.4% DO(nWL) 78 78 78 76 75 79 78 72 80 6.2 . 77 79 7.8 7.9 Conductivity (pinkos/cm) ______ _______ ______
__________ TemperatureCC) 248 245 249 246 249 24.2 247 245 250 24.6 249 246 25.0 24.4 pH (S!,J 5.021 7.91 8.021 7.721 7.981 7.588 8.01 7.711 8.011I 7.55 8.131I 7.921 8.041 7.77
- __ 7.D.9 77O_ _78m_
- 3 .8 .9 Lonnucnvnv mumao~eim, I 2:'1 I * "7J*3 7_hD '199n5es r - I_____ ~ _____-~ ~ - .:4 5~ -
I-rgemperatura ("C)
I -
24.81
-I -
.* 24.4* -
- 24.91
-0.
24.51 4
25.0[
- -~ -
S 25.0 I 24.6 24.91 24.51 25.01 24.4 nfl (SiU) 8.021
- 7.92 "8.031 %7.41 795S 7.70J 8~___ &os]__7s2J__To~j 77i 85.6% DO mnl)I
.......... I 78 .1 79 .4 j 7~_ -8.01 60 7.81 7.8l 7_Q... ]
70 r[*nndulotifvitv Ifinhnwfrlmt I -
Temperature ("C)
I-I pH (SU)_
DlO (mriL)
-a-Conductivity Isumhos/cmu) 100%
Hardness (mglL CaCO 3 )
ITotal Residual Chlorine (mn/L) I
]Temperature ("C)
I - --
IpH(SU)
DO f mo_/Ll ConductIvity (,unhos/cni) I 100%hIna !Alkalinity(ngfL CaCO 3)
Hardness (melL CaCO1 )
M i
Temperature ("C')
File: sqnl01_j02015chem.xisx Entered by: J. Surmner Reviewed by:
Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
, Species: Ceriodanhniadubia Client: Tennessee Valley Authority County: Hamilton Facility: Seanovah Nuclear Plant Outfall: 10.1 NPDES #: TN0026450 Project#: t O'V51 Dilutionpreparationinformation: Comm'ents:
Effluent volume (mL) 267..5 535 1070 2140 2500 Test organism source information: Test in~formation:
oOranismi : < 24-hours old Randomizinjg template color:
- 8.
Date and times organisms were born ID*Ol 0*A 'V* * } inculbaor number and shelf 2
- between: loaton Culture board: _____________"__lotin:
Replicatenumber: 1 2* 3 ]4 5 6' 7 sa 9 150....
Culturebhoardcup number, *, O ttIt, %.3I ~lt4 Ltlk*1 YWT bath. *0O.OtS Transfer vesselinfrmation:
lm ni) ]p= H
.02 Lot S.U. Ternperature = 1L..tvrg LSelenost Cse rum batch: Oi'2 AIS Daily renewal information:
Day . Date .Test initiation and feeding, MHSW Sample numbers used. -Analyst renewal and feeding, or batch used Outfall 101 Intake
- -termaination time ,
0 10-20-15 t* '*t-S- 6 i% ti,(3\ *._______*____-
- 2 io~-is~ ~i~-z~r' ~sc~-A~l 6 10-26-15 60Oo I~rb iS1 ioU. 05.
,7 10-27-15 .* t Controlinformatiorn Summary of test endpoints:
_______________________ Control-1 Control-2 Acceptance criteria ______________
% of *ale Adults: . C)?.07 <20% 7-day LCso __________,
'%Adults having 3rd Broods: !1 1.' 1Ct'7o >80% NOEC .l1001.
% Mortality: . .. Q [o. ______
- 2o% LOEC _________
Mean Offspring emal'e: ______o
- o*. 15.0Ooffspringlfemale ChIV *..100
_________o3 7, < 40.0 % ie ,
Ad.pend.n, R.~4.wb~
SOP ATI 1 - Exhiibit ATl 1.2, revision,11-01-14 Page 39 of 98
@* * .*T. *Page 2 of 7 Species: Cer'lodaphniadubia Client: TVA I Sequovah Nuclear Plant. Outfall1101 Date: 10-20-15 CONTROL-.1 ,Sun'* ival andRepJroductionData Day Yongrdu 1 1
- 2 3 4 Replicate .number_
5f 6 7 f819 10 Y
Adult mortality ioungprod.ced
__TAdult mortalityJ~ ,j..
k
- 1 o,~
I
.. Lk..
.odo
\
.- _L.
\ *
- o. 1o
- I 0 -
.o,. o.o.IL I
-x
'- I '-i k
... 3iYoung produeed O, 0' LQJO 010* 0 010* 0[*.I
[4IYoungproduced A 4*iiLA..- I___
6
__fAdul 1
tmortality You.pr.u.e Young produced j]Jp
- I '.-'L ...
'IL.1~7F V iof' o
r~.I~1V.I~
c: 0 i'.
___ Aduit mortality I ' i *l.. * '-I '-* I_'l-.l '. - I I'** I*
To]a youngproduced. .*"- '-. vl 1 iW l I SX X v.'*-
Fhnal Adult Mortality X for3*Broods
'*'....{
.~ "
I "*, I * ,I*
Nsre*:Alt mostlisy (L fi ve,D - dead), SB - split brood (sigle brood splitbetween two days), CO - cony over Co*yrlo csic over-with adult dinng trmsn5r),
CONCe 10.7% survival and1?eproductionData
____Replicate number ___.
2 I Youngproduced *I*~I0 1... .)O _
I Young'produced' I___ o l I ___1' o 1 L
[Adultmortali - *i \.---I -I.-- x I v-- L*-'.!k-I - I- 1 -
Yonprdce i4~ .1% -... .. 2. III -I 6 Adultmnortali7" ty *J IT'. __ LJ '*. j I S'i '
Total young produced I -' * [ :*
- j *' L * *I
- I Final AdultMortalit, .... I,-= .. -* * .: -.. -. ,.-
TV .liJX sonsgnsa assseusavervein sesasuwIerlaaaaseTI.
Con canfradon.
Mean Offspning/Feniele: I ~%~S
%Reduction from Control-I:.
ReSseby sailsyL Knwm.aI LA..- Page4Oof98 SOP ATI I - Exhibit ATI i*.2, revision 11-01-14
'*'*F.*....*i~st~lsollon~a¢,Page 3 of 7 Species: Cefiodapliniadubia Client: TVA / Seauoyah Nuclear Plant. Outfall1101 Date: 10-20-15 CONC,. 21.4% Survival and Reproduction Data I - . Replicate number SYoung produced O Q) c*) j* OcL* 101
- Adult mortality 2Yun.podce.... c 1 oA0101 Ir o-1.
- k. .- *- ~-* . ___ * - .
_ _ Adult mortality j . 1-1' [
- 1
'* t -- *-I 3 Young produced 100 0 .. Jy..I0[DI,.. ' '0 4 Young produced.Z 1~J.51.5. 'Jjj
__nal AdultiMortality I_-_ _ *. *s ____ .- . Ls - '-.
___ AdlMortait s-___ lity- __ __
6 Yunprdued~le J0 Of[fspring iIna0:I 14 ougprdce.8%
7Oe
-av Tot. [Young produced
,, 1-
- ) C I..3 -
- u+/-iv
%. and Rpou
- I tionData.
0%
9 -
4 L~oungproMean .Of,.sprIngl ema1e COC 428 SuouvanandprodducionDat 21 = - [ - - I ,..
1 Young produced 0 01m t Q, o***t*t 1'* I**
2Ttayoungproduced -I.0 *
- O &QjO1 ' 0 . 0
__na Adult mortalit *--
- L [** * '- "-- * -
P/ole:AGOIOotwtality tL live, IJ eeao~. liH = tpiIt DroooLelo8le0/000 spotocoweentWOOayO).LU = en Concenlrttlon:
% Mortality: .1.~
ffMean Ot~pring/Female: I $ *&
SOP ATI I - Exhibit ATI 1.2, revision 11-01-14 Page 41 of.98
- .,,s,. Page 4 of 7 Species: Cerlodaphniadubia' Client: TVA / Sequoyah Nuclear Plant* Outfall 1.01 Date: 10-20-15 CONC: 85.6% Survival andReproduction Data
- . _____ - Replicate aumber Da* __ _ 1 2 T314 516 7 18 9110 IFYoung produced O. (0 0100101010**)
[Aduit mortality t.... LJ , _ .-. - '.__I T[
Young produeed I O Id O OL * [I iI C 1 0 Lo. I O I 0
-IAdult mortality I - *- - I '- I t -. I
- 1 -I *-11 *-f I
_[ Adult mortality.I L- --. *- 1I* A[1 4 Young produced ~ __ -- ~__
SYoung produccd
___[',.'Adult mortality.*- IJ TL *I*
~L_-i1%--
- I*II~b J I.*
[.I__
'- I_ "-J-.- '~
6 Youngproduced [ * %* J '.C)" I I..t t l *1 I'P1' Total youngwpoduc~ed "*' .'* *-t *' I -"* " "* 1 * .*
F~lnal Adult Mortality -c: *- '- '- f %-, . s.*%-- j, *- __
lease: AOWSmommy IL ov~ I?= 0050). hki spill OWOO~eiogIe esosoaspin oesweon two 00)5), Lv CWOvover(o5asmemm v~aiilil00tOU0hilRO~~~
I%
3 Concentraton.
Mortality .. 0?".
MeanOffspring/Female:. I o*,
% Reduction front Control-i1: f-l *o CONc: 100% Survival andReproduction Data 1 ____....Replicate number_ _ _
Day Ii Young produced 1112 (0 J 0 3
0-4
- ... 10 iS
.x* 617
.n" ... -O 819 J....... 1
.110
___ Adult mortality 4 t . L_ __ L_.. "- 'I_ .-i
- __.___
J Youngprodced IAdult mortallty __
Oj Cb0 j
I . --
0Ig
-.. I L I*
'L-I.--_ 1 I*
-I..--
- oung produced Y
Autmortality tI t4_-_
0 ~
I
- t't
~~~x.
l) -d t* I-* I.
' ' I~
'JAdult mortality J '% , -I '--IkJ- I'- I '-1 V-I ',,1 __
Young produced 0 " ~~ f0 "~- I(1
'W "
FiIa Adult m ortality I
- _'- '-1, '_, 1 ' -. L. 1., .. j I.. '
Naote:Adult mortoilly (L = live, D - deed), SB - spilt brood (single brood split between two doys), CO= a iv over (aofsfbolo caroind over with adult during tran~lr).
Cotice, traikrn-S% Mortality: 07 SOP AT11I - Exhibit AT1 1.2, revision 11-01-14 Page 42 of 98
- L* n~mmenal~e1*rS~mlm.Page 5.of 7 Species: Ceriodaphuladnbki Client: TVrA / Sequoyah Nuclear Plant. Outfa~l1.101 Date: 10-20.-15.
CONTROL-2 *Survival and Reproduction Data
........ .... : ....... ... Replicate num ber____
Day ____ 112 3 4 516 7 8 91i0 Young produced .0 1 0 O,...
0 - 0 0 O
- O
__[ Adult mortality %] *- '-- *. j% ~ \._
- k-- '
JI +I Lo.. Il...... 2I .. i ... L -
.2
_I_ Young produced Adult mortality. ____ _--_
. [ )"
-I -I c .. - I *
"C*...
- IJ --.-
4 I Young produced I [AJ'A '14 0IO I ~II OW '4fO J. Adult mortality J k..l.ŽI--_-I '-[ 'I- I*- I'- '-"
6 Aduitmortality, Young produced ,
1* ,
Q"L
___T.
Q [
J L.oj*_ L
, .*.,I c"1,*
" ' Young produced * , " t.*
%l,*,,* * , ,'4 %l .t, Total young produced '*n* '**
Final A~dult Mortality %- \.. %--- %- *- a-. *- ** L-..
X for 3'aBroods .'~- E** 2 * *. .T -
A.ls/a Adul esunls *.'ive, D =dud), SnD apli brood (snl~e brood split betwama two days). CO: carry over (offspring carried ovu"vith adult during trasrer).
SConceniradon:
+ % Mortality:
- 07'F" CONC:: 100% Intake Survival and Reproduction Data 1.) ____Replicate number Day ____...._ 1 2 1 -3 1 4 1 5 1 6 7 1 8 9 10 IAdult mortality j *_. '-. i' _. __ '-*
__ Adult mortality * *-- JI JI *- _ *- _ *-
Yon mot]ty prdu ced
.I 'I,.-"IQ.. I"I0... ..*LL .... *O..I L...
u,.-,+~ I: . *....0 [ C2.. ..
p5-' L..C..
I t..:. ,,,,,,.+ I ...Xy....Lo.- .. 0 I o.- X...~..J...:..
3 Younporduce YonpodetAdultmortlily I " -I'.-J I ,-.it+I,'-- '+ I___ '- I,+.`
64 *Aduttmortality Young produced '-' H-iI'*-,~Ia4 IIl '*..I]'I
' ' '- '-SI'4I I *- - ll' Youpoue f' .1
- I *I+'4 -.
Tota young prodclued j+ 0 I .+1 q J L.
- "<"" .. (i
%+.. 1 C L..
Final Adult Mortality L* .L- * *. L.. %- L.. t. L.." .
Note: Adult rrortnliiy (L - lIve D 1 dead), SBt splitlirood (SaingiIroodlspllt between two days).CO= cawver toffsprsui ~~taed over withadult Onhgmsssitran.)
Mean Offspring/Female: . * '
5s4ve.nd.es posies,by % Reduction from Control-2: ,=o' ShinyLilnenpe SOP AT11I - Exhibit ATI 1.2, revision 11-01-14 Page 43 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated October 20-27, 2015
~*HI. S Verification of Ceriodaphniaz Reproduction Totals (a B ID Ezwh.oemenaI Inc.
TesOftgSoIul1oo~.
Control-I 85.6%
ii 1 R.~ Ikate number 4 isl 6 FTF~
Total Day 1 2n-3-r4 .5 6 8 9 10 J ID Day 1 ~ 2 1 3 o jo ~o 10 j0 J 0 0
0 2 1 .0 010 0 0 0
0 010 0 0 O,
0 0 I 0 0 0 0
0 0
0 0
OH to K
0 7 1:1 . - -. - -. -
3 0 0 0 0 0 0 0 .0 0. 3 0 00 (I 010 0 0 0 0 0 0i F' 4 5
6 Total 10.7%"
0 5
11 4
11 0
1414 13 1616 301 29 4
12 0.0 29 3
11 30 4
10 0
30 4
I3 0
1 531339J 2
O 0J0120 4115 .141171151 12 31 J31J31j32j28j301J 1
[0 1
1481 5
6 7
- Total 12 1413 0
4 17 33 38 0
6 18 0
6 15 18 37 32 13 1213 0
4 .5180 35 37 0
5 19 1I1111212 0
5 17 33 0
4 18 33 0
5 16 33 0
4 20
- 36 "123 0
48, 176 34 Replicate numberToa DY *1 2 3 4 5 6 7 8 9 10 Toa 1 0 0 .0 0 0 0 0 0 0 0 0 2 0 0 0" 0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0
- 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0. 0 4i 4 5 5 4 4 5 3 4 5 4 43 5 14 12 14 13 12 14 12 12 14 12 129 6 0i 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 15 14 15 13 16 15 16 17 16 154 7 17 17 19 20 17 20 18 19 17 18 182 Total 33 31 30 .30 30 32, 30, 32 .35 32 315 Total 36 34 39 38 32 39 35 36 37 36 362 3 .i0 0 0 0 0 0 0 0 0 0 21.4% _______ Control-2
- Replicate numberTol Day 1 2 3 4 5. 6 7 8 9 10 Tol 1 0 0 0 0) 0 0 0 000o o 1 0 0 .0, 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 .0 0 0 0 2 0 0 0 0 0 0.0 0 0 0 0.
3 0 0 0 0 0 0 0 0 0 .0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 5 4 4 5 4* 3 4 43 4 4 4 4 3 5 4 4 5 4 4 41 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 16 15 18 14 16 17 17 16 161 7 15 14 14 16 12 15 14 16 16 15 147 Total 33 3234 31 3531-3334 3232 327 T..'ota 30 28 30 29 29 32 29 33 32 30 302 42.8% ___________ _______ 100% Intake__________
Day 1 2 3 4 5 6 7 8 9 10 Toa 2I 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 .0
__ 0 0 0 0 0 0 0 0 0 45 0
6 0O 0 0 0 O 0 0 0 0 0 0 156 Total 32 36 32 34 34 33 33 34 31 33 332 Total 332
.2i1.~. J33 *~ *~* 31j32 File: sqn1 01_10201 5data~xlsx Entered by: J. Scquirer Reviewed by:.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated "0
October 20-27, 2015 Ceriodaphniadabia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 0 . Quality Control Verification of Data Entry, Calculations, and Statistical Analyses W" Environmental Testing Solutions, Inc.
Project number: 10958 Concentration _________ Replicate number Survival Average reproduction Coeffceint of Portent redoct'on from
(%) 1 2 3 - 4 5 6 7 B 9 10 (%) (offsprlng/female) v~~o ('A o~trl (%t)
- Control -1 30 29 29 30 30 31 31 31] 32 28 100 30.1 4.0 Not applicahle 10.7% 33 31 30 30 30 32 30 32 35 32 I00 31.5 5.2 -4.7 21.4% 33 32 34 31 35 31 33 34 32 32 100 32.7 4.1 -8.6 42.8% 32 36 32 34 34 " 33 33 34 31 33 1100 33.2 4.2 -1[0.3
- 85.6% 33" 38 37 32 35 37 33 33 33 36 1100 34.7 6.2 -15.3 1010% 36 34 39 38 32 39 35 36 37 36 100 .36.2 6.1 -20.3 Control -2 30 28 30 29 29 32 29 33 32 30 100 30.2 5.4 Not atpplicable 100% Intake 35 34 34 33
- 36 34 31 .- 32 31 32 100 33.2 5.1 -9.9 Outfali1101: MSD = Minimum Significant Difference DunneUt's MSD value: 1.743 PMSD = Percent Minimum Significant Difference PMSD" 5.8 PMSD is a measure of teat preoision. The PMSD is the minimum percent difference between the control and treatmnent that can be declared statistically significant in a whole effluent toxicity test.
Dunneft's MSD value: 1,282 Lower PMSD bound determined by USEPA (I 0 th percentile) = 13%.
PMSD: 4.2 Upper PMSD bound determined by USElPA (9 0 ehpercentile) =47%.
Lower and upper PlvSD bounds were determined from the 10th and 90th percentile, respectively. ofPMSD data fr'om EPA's WET Interlaborutory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Pr~otectioni Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratoty Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0I-004 and EPA-821-B-0l-005.
US Environmental Protection Agency, Cincinnati, OH.
File: aqnl 01 10201 5data.xlsx Table populated from associated "Verification.of Ceilodaphnla Reproduction Totals" spredadheet.
Spreadsheet entered by: J. Sujnner.
S Reviewed by."
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015
- * * *: Statistical A.nalyses Cenodaphnia Survival and Reproduction Test-Reproduction.
,Start Date: 1012012015 Test ID: CdPRCR Sample ID: T[VA/ SQN Outf'all1101 End Date: 10/2712015 tab ID:. ETS-Envir. Testing Sol. Sample Type: DMR-Disoharge Monitoring R~eport Sample Date: October 2015 Protocol: FWCHR-EPA-B21-R-02-01 3 Test Species: CD-Cerlodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 Control-I 30.000 29.000 29.000 30.000 30.000 31.000 31,.000 31.000 32,000 28.000 Control-2 30.000 28.000 30.000 29.000 29.000 32.000 29.000 33.000 32.000 30.000 10.7 33.000 31,000 30.000. 30.000 30.000 32.000 30.000 32.000 35.000 32.000 21.4 33.000 32.000 34.000 31.000 38.000 31.000 33.000 34.000 32.000 32.000 42.8 32.000 38,000 32.000 34.000 34.000 33.000 33.000 34.000 31,.000 33.000 85.6 33.000 38.000 37.000 32.'000 35.000 37.000 33.000 33.000 33.000 36.000 100 36.000 34.000 39.000 38.000 32.000 39.000 35.000 36.000 37.000 35.000 Intake 35.000 34.000 04.000 33.000 36.000 34.000 31.000 32.000 31.000 32.000 Transform: Untransformed 1-Tailed Isotoni.c Conc.% Mean N-Mean Mean Min Max CV%/ N t-Stat Critical MSD Mean N-Mean Control-I 30.100 0.0967 30.100 28.000 32.000 3.977 10 . 33.067 1.0000 Oontrol-2 30.200 1.0000. 30.200 28.000 33.000 5,362 10 10.7 31.500 1.0430 31.600 30,000 35.000 5.238 10 -1.837 2.287 1.743 33.067 1.0000 21.4 32,700 1.0828 32.700 31.000 35.000 4.090 10 -3.412 2.287 1.743 33.067 1.0000 42.8 33.200 1.0993 33.200 31.000 38.000 4.212 10 -4.066 2.287 1.743 33.067 1.0000 85.6 34.700 1.1490 34.700 32.000 38.000 6,233 10 -6.036 2.287 1.743 33.067 1.0000 100 3,6.200 1.1987 36.200 32.000 39.000 6.080 10 -8.005 2.287 1.743 33.067 1.0000]
Intake 33.200 1.0993 33.200 31.000 36.000 5.080 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.65447 1.035 0.13015 -0.266 Bartlett's Test indicates equal variances (p = 0.34) 5.7073 15.0863 The corntrol means are not significantly different Ip = 0.88) 0.1 5703. 2.10092
,Hyrpothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.74259 0.08789 47.7867 2.9037 7.7E-10 5, 54 Treatments vs Contrel-1 Linear Interpolation (200 Resamples)"
Point % 50 95% CL Skew 1005 >100 1010 >100 1C15 >100 1.0 IC20 >100 0.8 IC25 >100 0.8 I040 >100. 0.7 IC50 >100 0.6 0.5 S034 S0.2 0.1 0.0
-0.1 '. . . . = .
-0.2
-030 50 100 150 Dose-Response Plot 45 405 30 1-tail, 0.05 level
- =25 of significance
-20
- 10
.1 a a; a o* =
- p *File: sqnlOl102O15dat~ylsx ihse by .U8 Page 46 of 98 **~*_*
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated October 20-27, 2015 0
0 Statistical Analyses En~IronmeflthIte tlnuS~hdi~n; Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 1012012015 Test ID: CdFRCR Sample ID: TVA I SON Outfall 101 - Intake End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sal. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 201,5 Protocol: FWCHR-EPA-821-R-02-01 3 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1. 2 3 4 5 6 7 8 9 10 Control-i 30.000 29.000 29.000 30.000 30.000 31.000 31.0.00 31.000 32.000 28.009 Control-2 30.000 28.000 30,000 29.000 29.000 32,000 29,000 33.000 32.000 30.000
- 10.7 33.000 31.000 30.000 30.000 30.000 32.000 30.000 32.000 35.000 32.000 21.4 33.000 32.000 34.000 31.000 35.000 31.000 33.000 34,000 32.000 32.000
- 42.8 32.000 36.000 32.000 34.000 34,000 33.000 33.000 34.000 31.000 33.000 85.6 33.000 38.000 37.000 32.000 35.000 37.000 33.000 33.(000 33.000 36.000 100 36.000 34.000 39.000 38.000 32.000. 39.000 35.000 36.000 37.000 36.000 Intake 35.000 34.000 34.000 *33.000 36.000 34.000 31.000 32.000 31.000 *32.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max -CV% N t-Stat Critical MSD Control-I 30.100 0,9967 30.100 ,28.000 32.000 3.977 10
- .Control-2 30.200 1.0000 30.200 28.000 33.000 5.362 10 *
.10.7 31.500 1.0430 31.500 30.000 35.000 5.238 10 .
21.4 32.700. 1.0828 32.700 31.000 35.000 4.090 10 42.8 .33.200 1.0993 33.200 31,.000 36.000 4.212 10 85.6 34.700 1.1490 34.700 32.000 38.000 6.233 10 100 36.200 1.1987 36.200 32.000 39.000 6.080 10 Intake 33.200 1.0993 33.200 31 .000 36.000 5.080 10 -4.058 1.734 1.282 Auxiliary Tests . .Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92371 0.668 0.31324 -0.9934 F-Test indicates equal variances Cp= 0.91 ) 1.08475 6.54109 The control means are not si nificantiy different (p= 0.88) 0.15703 2.10092 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedestlo t Test indicates no significant differences 1.28211 0.04245 45 2.73333 7.4E-04 1, 18 Treatments vs Control-2
"' Dose-Response Plot 45 40 35 303 -
- 25 TI I I I-I-------------------------------
I 1-tail, 0.05 level of significance 0O I- 2 0.
I- ~ CD CO 00 rj, o c~~j IL)
- e.j 'e' CD
- Page 47 of 98 File,: sqnl 0lO12015data.xlsx Entered by: 3. Sumner Reviewed by:
Page 6 of 7 Species: Ceriodaphniadubia Date: 10-20-15 Client: TVA / Sequovah Nuclear Plant. Outfall 101 Daity Chemistry:
I (Analvst identified for each day. nerfhrmed DayD.O_
M oH.
and 'ondiic~tiuit.- .~apo.IwemPn'o
--- - - .--- .-. ~
rnnlv.*
-. Ii---
0 I 1 i Analyst '
Concentration ] Parameter DO (mn/LI itdiictivity (ra*L*
(tunhos/cml
- DO CONTROL *Alkallnity (mg CaCO i/LI
- Eardness (mCaCOi/L)-
- Temperature (OQ2 4 - - U -- S -
oH (S.U.)
DO (snaL) 7.~1 II *.* I *, -'. ':
10.7% Conductivity
- Temperature (00) "*AA 1~
1l~i -~
tq.
~
I
- *1 .
~!i(~LI ~¶2 ____ 7.97 2 1.4% Conductivity I ~ -
0 1
- Temperature ( C) l'~ b.
DOm
____ LZI~
it i.'iq 1 DO (mal/L) II 42.8%
Conductivity
- Temneratuiret°C)
J~LS I.- 4~
pH (SU.)
DQ~g/L) -74 85.6% Conductivity 1 ~
- *Temperature(OC- - A..% U IS -fl.o L. I _ .*O Ii:**
Conductivity 100%Alkailinity
- Hardness ,*. .
- TR chlorine (mg/L) **' t10
__________ *Te )erature(C _. 1. 0 -.
- Conductivity i - -
100%
- Intake *Alkcalinity (mgCaC 3IL
=,.*
=== ==* *TR chlorine
- (mgfL). C 0.oIQ -* -
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual ojlorine performed by the analyst identified on the bench sheet specific far each analysis and transcribed to this bench sheet by:" :"
SOP AT11I - Exhibit ATI 1.2, revision 11-01-14 Page 48 of 98
- * '*""'*'*Page 7 Of 7 Species: Ceriodanhniadubla Client: TVA / Sequovah Nuclear Plant. Outfall1101 Date: 10-20-15 I
Day (Analyst identified for each day. n~erormied pH. D.O. and conductivity measurements only.) Im II 3 I. 4 I .1 5 I Analyst Concen-Paaer trationt "_____" __
pH (S.U.)
DO (mg/L)
Cohnductivity
(~mos/cm)
CONTROL *AN~aljnity (nig CaCO 3 IL)
- Hardness (nig CaCOilL) rI ~
- Temnerature (0C*
-, -- .- i~i .z. - -
DH (S.U.)
DO(rng/L) 10.7% Conductivity (jimhnslcm)
- Tamnan.o+....o 1"Cfl
- a. -
s
~ -
= fl.np.A S.... - ~
pH (S.U~)
DO(mgfL) 7 21.4% Conductivity ~qLJ
__________ *Temperature (0C)
- p11 (S.U.)
- DO (mg/L) 7.
42.8% Conductivity (prnhcslcm)
___________ *Temperature (00) pH (S.U.) :
85.6% ConductIvity (unhoskm) t Temperature (0C)
- V uR (S.U.i
. ..... _
- I-Conductivity 100%
- Hardness (mig CaC03L).
- TR chlorine (ragiL)
- Temnerature (°C1 m m I
pH (S.U.)*
DO (m*./L' 100% *Alkalinity Intake SConductivity
- Hardness
- TR chlorine (mg/IL)
II SInitial I ina.I Xnitia I Final I Initia . ialIII~nilia, II inF
- Temperatlures performed at the time of test initiation, renewal or termination by the analyst identified in thle Daily Renewal ,
information table located on Page I. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench "l sheet specific br each analysis ad transcribed to this bench she-etby: ._._.'
1 t**
S6P AT11I - Exhibit AT1I.2, revision 11-01-14 Page,49 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated October 20-27, 2015 Ceriodaphrniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Enviro.nmental Test~ing SolUtions, inc. Project number:. 10958 Concentration Parameter Da0D 1D a a4Da5D 10958
______________ nta ina Inta ia nta ia nta ia nta ia. Iiil Fnl I il Final DO (mulL) 7. . . 7 7 . . 79 79 79. 77 79 76 7.9 Control Alkalinity (mg/L CaCO3 ) 6 . 6 6 Hardness (mgIL CnCO3) 88 888
_______Temperature !CC) 248 2.,49 2. 47 2. 4. 49 2. 52 2. 51 2. 25.2 Hi 5 .2 79 .2 7.2 79S.7 . 7.9 79U.0 807 81).9 80 8.01 DO (mu_.L) . . . . 78 78 7.8 .. . . . . . .
10.7% _Conductivity 0zmhoslem) 31 1 9 30.21* 9 0 *, :dl.'*
_______Temperature CC) 248 .2. 49 2. 4. 45 2. 51 . 2.0 2. 47 2. 50 25.0
- ., (sU) 8.2 79.80 .2 7.8 79.0 .8 801 80 . .9 80 8.01 DO (mulL) . 78 79 78 78 75 78 78 79.0 79 77 79.8 7.9 21"4%/ Conductivity (nznhos/cm) , _____ __________24 8027 *29
,Temnperatre °C) 48 2. 50 2. 49 2. 4. 51 2. 52 2. 49 2. 25.0 pH (S 82 79 82 79 .8 79 .0 .8 81 89 83 80 80 8.01 4.o DO (me/L) 787-9 79 76 . . . 0 0 7 0 79 7.9 4.% Conductivity (jumhosfm) _____ 2tY' 267 Temperature (°C) 24,5,5 48 2.9 2. 49 2. 50 20 27 2 50 24.9 pH(SU) 80,0,0 79 .5 7.9 80 .0 0 0,85 82 80 8.01 Ds6/
o (mnlL) 78 7 9 79 76 78 78 80 8 0 7 9 79 7.9' 8.% Conductivity (junmhos/cmn) 2,6 ____ 31 0
_______ emperature ( C) 24'51 20 2. 5. 48 2. 50 25 4 4 4 50 25.1 pH (SU) 803 .80 8.3 79 7.3 80 8.3 79 8.1 80 78 80 807 7.99 Do (mgIL) . . . . 78 78 7.8 76 80 7. 8 7 . .
Conductivity (nnlos/em) 17
- 8 7 8 8 8 8 100% Alkallnity (mglL CaCO3 ) 6 7373.
Hardness (mg(L CaCO3) 90 73 69 Total Residual Chlorine' (mulL) < .1 .<00- <01 S Ternperature 248 .2. 5. 49 2. 2. 50 2.2 2. 51 24 4 51 24.9
- H ISU.80 .0 80 .6 .8 80 .3 80 79.4 89 84 80 8.03 DO (mulL) 78 78 80 79 76 78 78 77 .. 80 78 77 79.0 8.0]
Conductivity (pmlaos/em) 17 7 7 8 8 8 9 Hardness (mgIL CaCO3 ) .6.7 . .6 Temperature (0C) 47 2. 50 2. 49 . 2. 49 2. 49 2. 49 2. 24. 2.9 File: *sqn101_.102015cliem.xisx Entered by: J. Sumner.
Reviewed by:
Page 1 of 6
"* Chronic Whole Efflueni tToxicity Test (EPA-821-R.-02-013 Method 1000.0)
- 1 .."-" . .~
pecies: Pimev/hales promelas
- lttA~itI. Jt* nlog V dIl~tY tLkUtlIUI l County:
Outfall: Ilamilton 101 Facility: Sequovah Nuclear Plant NPDES #: T1N00264.50.
Project #:
Dilutionpreparationinformation: .Comments:
Dilution prep (%6) 10.7 21.4 "42.8 j85.6 " 100' Each concentration was UV-treated Effluent vohrnie (niL) 267.5 535 1070 2140 2500 for 2 minutes to remoVe pathogenic Diluent volume.(iL.) 2232..5, 1965 1430 I360. 0 . .Interferences..]
Total volume (niL) ... 2500 2500 2500 2500 2500 "Testorganism'information: .... Test information: .... __
"Organism age: itA -* "t \*,1 %* . *L Randomizing template:' ______,_
Date and times organisms 10-19-15 1600-1700
- In.cubator number and w~ere born between: .shelf Iocation: ______
Organism source: ATOX(Batch Pp: !10-19-15 "Arterma OHM number: *C11M836 1)rying informationfor weight Transer pH= bol~determination:____
~
infrmatin:
Tranferbowlinfrmaton:pH= *.oo' ",U. Date / Time in oven: I 1,')*..4 * .
Temperature = X... * ° Initial oven temperature: J ¢_ *" t Average transfer volume: 0.1749 mL " Date/'Time Out of oven: J lo*I-t*
Final oven temperature: I a"CV(
____________ ________________Total drying time: "j -*
Dailyfeeding and renewal information:
Day Date '" Morning feeding Afternoon f'ee~ding Test initiation,. Sample numbers used MHSW renewal, or batch
_____ termination used
... Time Analyst Time. Analyst Time Analyst* Outfall 101 l Intake..
3 10-23-15 o>* ..] * *'Ac ...4...... N ..4j".. tI¶Ui2,1 t1 ~ icD2&.* 14 IO'20" S 6 10-26-15" '~frr~ A I, (04b ISIb1~ .cA tts-'l*-tS
[ 10-27-15 I~2JI~i Coqtrol information: Acceptance criteria Summary of test endpoints:
I%Mortality..
I vrg egtprinitial larvae:
Average weight per surviving larvae:,,
I 0120%
I 0 S¶ O.6g
,, Ž,0.25mg/larvae j]7-a NOEC LOEC LCs5 .
I[ til
[0 ChV "7 jtj7.
IC2 5 ~
SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 51 of 98
, ..... Page 2 of 6 Species: PimeDhale* promelas Client: TVA / Sepuo~vah Nuclear Plant. Outfall 101, UV-treatedl Date: ¶0-20-15 Survival and Growth.Data Day CONTROL - 10.7% " 21.4%
_ _ _ _ _ _A B C D E F C H I J K L 0 10 10 10 ]10 10 10 10 10 10 10 10 10 1 0 1bl* ~0 *, 0 10 t0O 10 10 tq
- 7, I0 *O O i 10O~ t 1tO (0 CbO IQ
"° Ir i0 1 0 10 JO 1010 lb i0 1O
- 6 1 10O '0 10 1 0 #0 #0 t 7D l0 ID II Q ' 1 0 10 ! 0 ijO 0 1 A= Pan weight (mg)... ... I ....
Tray color code:; tAY*,e., -CA'..fl' Analyst: ___ ___ ___ ___ 1 {.IIeo %.010 I(,*,* 14, 2..
I C.'.I l*.14 1(45.$* . t l%.'l 1i*.t"
- 7* 7 *2 Date: t9o - 2_.g -
B = Pan + Larvae,weight (nig)'. *.
Date: ___ -____ ____ ____
C --Larvae weight (mg) =--B - A Analyst: .*
Weight per initial number of larvae (mug)
= C / Initial number of larvae Hand calculated. C,.
I, '0 Analyst:. /3
= = I= 3 Average weight per Percent redaction iniia nmbe o Ifrom control (%) *o.,
larvialnne(r of _____
Comment codes: c --clear, d = dead, fg =fungus, k = killed, mn = missing, sk = sick, sm = unqusually small, ig =unusually large, d&r = decanted and returned, w = wounded.
Comment: v
. .. SOP ATI2O - Exhibit AT2O.3, revision 11-01-14 fi rage *z oT *
.... Page 3 of 6 Species: PimephalesDromelas Client: TVA I Seqiuovali Nucle~ar Pian[, Outfall 101* UV-treated Date: 10-20-15
____________________ _____ _____ _____Survival and Growth Data _ _ _ _ _ _ _ _ _
..... Day - 42.8%7 -a8.6% -, - 1oo% , ..
. _...._ _...... _ -_M .. N 0- P ... Q . R 'S., T U _V W X 1o o l l0b 10 lO toO 10 lO lO 2
__________ 10
- lk* 10 0 II) 10 I0j 1t* 10 to I* 1(
S !0t t0 t0 to. tO' to tO * (0 tO tO t
- 6. *0 f* t10 l* 10 10 tO '0 1* 80 7
A=Pan weight (rag)
Tray color code:: *,iWV1 £ GI tll IFI B = Pan + Larvae weight (Dig)
Date: l-*)fg-j*- u5*.
C =Larvae weight (mig) *-B - A ...
HncaultdAnalyst: A Weight per initial number of larvae (Dig)
= C /Initial number of larvae -,.* * *0 * .@ **,* *, @ .'
Hand calculated. * " * ° b * °
- Average weight per Percent reduction initial~number of. fromcontrol (%) *
- o* !.' **
Ue'a°* -*oa7i :
larvae (nrag)__________ ______
Commnt cnodes: c = clear, d = dead, fg = fungus, k = killed, m --missing, sk = sick, sm = unusually small, Ig =unusually large, d&r = decanted and returned, w =wounded.
..C..... m e t:, . . . . "' 2. . . .L SOP AT20 - Exhibit AT20.3, revision 11-01-14 53 of 98
- ...... Page 4 of 6 Species:
Client: TVA Pimeohales mn'omelas
/ Setusoyah Nuclear Plant,.Outfa~ll iDE UV-treated Date: 10-20-15 Day 10% Intake
_______ ___ Y Z AA B 0 10 2 i
'7o10 A =f Pan weight (rag)
Trayceolorecode:: ]rt)O.-L ]lLa..
Analyst: __________ I1 SO~
1C*.4L SDate: O'P2A.q-1i5 B- P'an + Larvae weight (mag)
Analyst: ., .*$ 5 C =Larvae weight (mag) = B - A Hand calculated* ~
Analyst:
- Weight per initial number of larvae (mag)
=f C /Initial number of larvae Hand calculate* .,
Analyst: *
- Average weight per IPercent reduction from control (%)
initial number of 0 **
Comment codes: e= clear, .d = dead, fg = fungus, k = killed, mn = missing, sk =sick, sm = unusually small, Ig --unusually large, d&r = decanted and returned, w =wounded.
Comments:" -.
R,~q,,,by
~MIoyE ,,.n SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 54 of 98
TVA / Sequoyahi Nuclear Plant, Outfall 101 U V-treated October 20-27, 2015 Pimephalespromekis Chronic Whole Effluent Toxicity Test EPA-821-Rl-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc.
)Preject unumber: 10958 Creeraltre 1uu%) Rel~cate Intltdmarbeel14d reu m rer-orl e A PmmweteJht*ergB- Pan÷Larvae tLenenenht(umgl Weighl/Ssucnvim tteruwetght/t Certflehatefse~aisb Wrtcht/1n.itiat mber Mea,,srtval *Mea lgIht/lteVl Ont.otiret Peresett ,dsetierfrem larvae weight~egl -A-B nuaerohtlccveelmgl SuumeigenuI'eof *m~P~I n 01alts-erer(g). (I%) neambeoflanve. -.aeathrn a..,eagc conatint ('.)
tern (erg ,=,...ron...tt%) (ag) Pea.jcer .O.,,.ct A I0 10 16.81 " 22.74 5.93- 0.593 0.593 Contcal B 10 10 16.06 22.06 6.00 " 0.6020 0.581 8.1 0.600 100.0 0.581 8,1 . Not appllcable C 10 10 15.27 -21.45 6.18 0.618 0.618
________ D 10 10 16.13 21.25 5.12 0.512 . 0.512 _ ________________
8 10 10 14.72 20.1I4 5.42 "0.542 0.542 0.% F 10 10 16.11l 23.06 i 6.95 0.695 0.9 200.69510.0591.0-.
7%--. G 10 10 16.66 22.32 5.66 0.566 0.9 20 . 0,56610.D.91.0-6 10 '0 1.6.35 271.95, 5.58, 0.558 _______ 0.558 ____ ______ _______
I . 10 10 16.36 22.91 6.55 0.655 .0.655 2140 Jr 10 10 15.97 21.70 5.73 0.573 066570.57310. 0.657-4
'14K 10 10 16.36 22.:33 . 5.97 0.597 0.0 70.597 100 06657-.
L "10 10 16.24 22.23 5.99 "0.599 _______ 0.599 ____ ______ _______
M 10 10 16.30 22.77 . 6.47 0.647 0.647
- 4. " N 10 10 16.19 22.36 6.17 0.617 0.0 290.617 10O 0671. ,.
0 10
- 10 15.71 20.65 4.94 0.494 0,611290494 100 067 29..
________ P .10 . 10 16.06 22.76 6.70 0.670 .0.670 ____ ______ _______
Q 10 10 . 15.98 21.57 5.59 0.559 0.559 85.l R 10 10 15.19 21.33 6.14 0.614 .91540.614 100 05154-.
856 10 10 15.95 21.63 5.68 0.568 0.9-. 0.568 100 05084-.
____-_____ 0 10 18.45 22.68 6.23 0.623 _ _____ - 0.623 ____
'U 10 10 16.75 . 22.88 6.13 0.613 0.613 10P .V 10 10 16.34 22.43 6.09 0.609 068700.609 100 06870..
10"a W 10 10 15.00 21.77 6.77 0.677 0 .61. 0.670.0 0617.0..
________ X 10 10 15.88 21.61 5.73 0573 ________ 0.573 _____
Y 10 10 15.79 21.82 6.03 0.603 0.003 00 Itke Z 1 10 16.41 21.72 5.31 0.531 0.531 100 0*861Z l taake AA 10 10 15.04 20.51 5.47 0.547 0.568 6.1 0.54710.0566.22
________ BB 10 10 16.06 21.97 5.91 0.591 _______ _______ 0.591 ______________
Out fall 101: MSD = Minimum Signific.nt Difference Dennett'S MSD valom PMSD = Percent Minimum Significant Differecee 0.0919 PMSD;r 15.5 ?1SDf is a oreasure of Sectprecision. Thal'MSI) is 6,e minimumr percent difference between the control and treatment that can be declared statistically signific.ant ina whole effl'uent toxicty test.
Intale:
Dunnettea IbSD value: 0.0566 Lower 1'1.SD bound determined byUSEPA (l0t], percentile) - 12%.
PMSb-D:" 9.7 Upper 8'MSD bormd deteried by USEPA (90th percenttle) = 30%.
Lower and upper PMSD bounds Were determined tfrott the 10th and 90th percentile, respectivey, of PMSI) data from EPA's WET Interlahorelory Variability Study (USEPA, 2001 a; USEPA, 2001b).
- USEPA. 2001a, 2001b. Final Report:Irtterlaboratory Variability Study of EPA Short-torn Chronio and Acute Whole Effluent Toxiceity Test Methods, Volumes I and 2-Appendik EPA-821-B-01 -004 and EPA-821-B-01-t05. US wrtioenmental Protectqion Agency. Cincinnati, 0H1 File: scqn1011 02015fdata-uv.rdsx Entered by: J. Sut~8r Reviewed fby:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated o~o T October 20-27, 2015
~Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10120/2015 Test ID: PpFRCR Sample ID: TIVA/ SQN Outfall1101 End Date: 10127/2015 Lab ID: ETS-Envir, Testing Sal. Sample Type: DMR-Discharge Monitoring Report Sample Date: October2015 Protocol: FWCHIR-EPA-821-R-02-013 Test Species: . PP-Pimnephalespromelas Comments: UV-treatad Cone-% 1 2 .3 4*
D-Control 0.6930 0.6000 0.6180 0.5120 10.7 0.5420 0.6950 0.5660 0.5580.
21.4 0.6550 0.5730 0.5970 0.5990 42.8 0.5470 0.6170 0.4940 0*6700 85.6 0.5590 0.6140 0.5680 0.6230 100 0.6130 .0.6090 0.6770 0.5730 Intake 0.5030 0.5310 0.5470 0.5910 Transform: Untransformed 1-Tailed . .Isotonic Cone-% Mean. N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5808 1.0000 0.5808 0.5120 0.6180 8.098 4 .0.5989 1.0000 10.7 0.5903 1.0164 0.5903 0.5420 0.6950 11.951 4 -0.249 2.410 0.0919 0.5986 1.0000 21.4 0.6080 1.0435 0.8060 0.5730 0.6550 5.732 4 -0.652 2.410 0.0919 0.5988 1.0000 42.8 0.6070 1.0452 0.8070 0.4940 0.6700 12.9;15 "4 -0.688 2.410 0.0919 0.5988 1.0000 85.6 0.5910 1.0176 0.6910 0.5590 0.6230 .5.444 4 -0.269 2.410 0.0919 0.5988 1.0000 100 -0.6180 1.064'1 .0.6180 0.5730 0.6770 6.999 4 -0.977 2.410 0.0919 0.5988 1.0000 Intake 0.5680 0.9780 0.5680 0.5310 0.6030 8.068 4*
Auxiliary Teats Statistic Critical Skew Kurt Shepiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98963 0.884 -0.0913 0.47811 Bartlett's Test indicates equal variances (p.= 0.81) 3.5619 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChiV TU MSDu . MSDp MSB MSE F-Prob "d Dunnett'sTest 100 >100 1 0.09189 0.15823 0.00076 0.00291 0.9287 5, 19 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(E~xp) Skew ICO5 >100 1C10 >100 I015 >100 .1.0 IC20 >100 0.9 1C25 >100 . 0.8 IC40 >100 I050 >100
- 0.7 0.6
- , 0.5 0O.4 0.3 0.2 0.1
-0.1
-0.2 . . . . . . . .
0 50 100 150 Dose %
Dose-Response Plot 0.7 0.6 0.5 1-tail, 0.05 level of signilicance
- 0. * .
0 - N 10-0
. File: qsq101102015 data-uv.xlsx Entered by: J1.Sumner Reviewed by: _
aae 56 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake
- UV-treated October 20-27, 2*015 Statistical Analyses EnvIonmsntal Testi ngSobjilons, Inc.
Fish Growth
- Sampleand Survival Test-7ID:
Day Growth Start Date: 1012012015 Test ID:LarvalPpFRCR TVAI SON Outfall1101 - Intake End Date: 10/2712015 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2015 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pirnephales promnelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5930 0.6000 0.8180 0.5120 10.7 0.5420 0.6950 0.5660 0,5580 21.4 0.6550 0.5730 0.5970 0.5990 42.8 0.6470 0.6170 0.4940 0.6700 85.6 0.5590 0.6140 0.5680 0.6230.
100 0.6130 0.6090. 0.6770 0.5730 Intake 0.6030 0.531.0 0.5470 0.5910 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Mini Max CV% N t-Stet Critical MSD 0-Control *0.5808 1.0000 0.5808 0.5120 0.6180 8.098 4 10.7 0.5903 1.0164 0.5903 0.5420 0.6950 11.951 4 21.4 0.6060 1.0435 0.6060 0.5730 0.6550 5.732 4 42.8 0.6070 1.0452 0.6070 0.4940 0.6700 12.915 4 85.6 0.5910 1.01 76 0.5910 .0.5590 0.6230 5.444 4 100 0,6180 1.0541 0.6180 0.5730 0.5770 6.999 4 Intake 0.5680 0.9780 0.5680 0.5310 0.6030 8.068 4 0.437 1.943 0.*0566 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.8848 0,749 -0.9219 -0.3332 F-Test indicates equal variances (p = 0.62) 1.8616 47.4583 Hypothesis Test (1-tall, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic tTeat indicates no significant differences . 0.05665 0,09755 0.00033 0.0017 0.67716 1, 6 Treatments vs D-Control . .. Ds-epnePo fIR
- 0.7-1-tall, 0.05 level
- 10.5 of significance 19 0.4, 00.
0.2E 0.1 04 CD 0 0 ~-
(SI e~J
.~. CD -
Cs File: sqnIl01102015data-uv.xlsx Entered by: 3. Sumner Reviewed by:
57 of 98
C) ~
~
Page 5 of 6
.Species: Pimenhales promelas Date: 10-20-15
- Client: TVA / Seau~ovah Nuclear Plant. Outfall 101. UV-treated Daily Chemistry:
[ Aaytidentified for each da, , performedpH, D.O0 and oonductivity, measurements only.)
1_____Lls oil (S.U.)...... ~.O5 II 1.95 IJ.~1'i b19 I-l.Vl5 iij.qi IIt======.J.
r---- r -a - ______
DO (mglL)
Conductivity Laa CONTROL *Mkalinity -
UV-treated (mg CaCO3 /L)
(~+/-mhosfcm)
- Hardness 57~ -
(ing CaCO~1L)
- Temverature (0C) 2.'AA ~i'L4.¶a 'i~4A Fi~7W - v L pH (S.U.) II -7 ~ c~7i e.oO 171 Ict~][
I-I.
DO (ngdL) 10.7% Conductivity mhos/crn _______
- Temnerature (°C) a.
~V2- I - -
14A.. N pH (S.U.) =4~I
- 00 II-! .I *!- I1.c-*
-~
II 7'* I DO (ma_/L')
21.4% Conductivity
_(*imos/em)
- Temoerature (°C) ~O L. a i . -- = - _. _!, -- * -- ..
- plJ(S.U.) Ii-q7 B?~W N DO fm*L) -I 42.8% DO (maiD Conductivity N B.
(i+/-mhos/cm) o'.25
- Temperature (0C) LS .O
.1 'U. -~
oH (S.U.I .0I
...... -- II----------
!DO (mWL) . I' -I S5.6% Conductivity hemhoslcm) -
1.o 4
- Temperature (°C) 4 1~~A *-1~
nHl (S.U.' II 1..Qt.~ I - -- "Ul* Vm*=ml=mmll==t!
DO (maiDL A a
Conductivity (amhos/cmn)
.5 I1~3 100% *Alkalmnity S*Hardness*
]*TR chlorine (rag/.)
- Temoerature (°C)
,60I-~
4 -7 pH (s.u.)
Conductivity 7.* .. %C 7..
100% *All~lifiuty*
Intake ~ gCC3L
- Hardness "
,dmtperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
/R,*.-*kinlity* hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet b.
XoI'o cs..* . _.Total residual chlorine was performed on non-treated Outfall 101 andlIntake samples.
\ oIK,,98" -*age 8 *SOP AT20 -Exhibit AT20.3, revision 11-01-14
...... Page 6of 6 Species: _Pi.meyhales pr'omelas Client: TVA / Sequovah Nuclear ]Plant. Outfil~l t01, UV-treated Date: 10-20-15 Day M
a la (Analyst identified for esoh day, performed pH .O ndcodctivit measurements only.) H I B Ana1ys~
Coneen-tration IParameter pH (s.u.) _ 'i -i-. I DO (mr_/L)
S- II- -i--A-----
Conductivity CONTROL (umhoslcm)
UV-treated *AlJtalinity (ma CaCQiIL~ _
- Hardness (inc CaCOSL')
- TempDerature (0 C)
DO(rng/L)
!nhos/cm
- Tern erature('Q ~. A
- 1,~SI.t~ 1. ~6 L ~ jJ j,4 ~ 4 C2.r*n I LA 1 ~MjL7q~J~LJ DO (mrIL) 17* r 74' 21.4%
Cond:ctivity
- Temmnarature (0 C1 I ~
II ,A..u, I ,~i II ir~i-~-
DH (S.U.) II 1.10 I %.O1 II Q~ 1 ~ II 7.9* I a304*
L 11 $ - H DO (mWL) li tl 42.8%
I Conductivity (j+/-nihos/cm)
- Temnerature tPC) ~S.O -IA.-t udkmmmmimalma* ...
isa~
.. [*a IpH (S.U.) 0 lv~ ~IA~ Ii - - I II L~T - -
DO (mgfL) 85.6% Conductivity (i+/-mhos/cm)
- Temnerature (°C*
9 -. - ~ - ~*--. ~m....
DH (S.U.) 8a03 II 7A~* I J.92 DO (mg/L) 7',
I Conductivity I~3
- AUwlinity U 100% 15
~HardnessH
~Th eblouine (mg(L) ~co.lO K. I
---i --- -- _ ,,
"1-91 II 7* .0 Conductivity
- Aflkalinity
-Iso 100%
Intake
- Hardness*
- TR chlorine (mg/L)
'A oow
~t I -aD ~ ,~ ~q~*
- liem eratnreru) 4*.t ' *) " ** L ..*2 I -a £... *.5*. ,
- peratures performed at the time of test initiation, renewal or termination by the analyst identified inthe Daily Renewal Information table located on Page 1.
I(!r.yo* jk51 ity, hardness and total residual chlorine performed by the analyst identified onf the bench sheet specific for each analysis and transcribed to this bench sheet
- __.. Total residual chlorine was performed on non-treated Outfall1010 and Intake samples.
"-- Page 59 of 98 " . "SOP AT20 - Exhibit AT20.3, revision 1 1-01-14
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated
~October 20-27, 2015
¢DW E. r *l**Pimephales promelas Chronic Whole 1Effluent Toxicity Test
~EPA-821-R-02-013, Method 1099.9 0
- Daily Chemical Analyses Project number: 10958 ia~ A EnvironmentalTesting Solutions, Inc.
ConcentrationParameter DaO Dal Da2 Da3 Da4 DaS Da6
____________ ________________________ Initial Final Initial Final Initial . Final Initial . Final Initial Final Initial Final Initial Final pH (SI]) 8.05 7.95 7.99 7.79 7.95 7.91 7.99 7.69 7.99 7,64 8.12 7.92 8.03 7.80 DO (malL) 7.7 7.7 78 7.7 7.7 7.9 7.9 73 78 6.7 7.9 7.7 7.8 7.8 Conductivity (junhoslcm) 311 325 308 32.5 302 313~
Control Alkalinity (mgJL CaCO 3 ) 63 63 63 .
Hardness (mglL CaCO3 ) 86 86 86 ~
Temperature (IC) 24.9 24.6 24.9 24.4 24.9 24.5 24.8 - 24.7 24.9 24.6 24.8 24.4 24.8 24.6
~H (SLY) 8.05 7.92 . 8.00 7.74 7.96 7.90 8.00 7.69 8.00 7.60 - 8.13 7.93 8.03 7.80 10.7% DO (maiL) 7.8 7.7 7.8 7.7 7.9 7.9 7.9 7.3 7.9 6.7 7.8 7.8 7.8 7.7 Conductivity (junhoilcm) 298 '. +/-.. . 312 ,.. 295..............310 291 300
___________ Temperature ('C) 24.9 24.6 25.0 24.4 24.9 24.6 24.9 24.7 25.0 245 24.8 24.3 24.9 24.6 nH(SU) . . 8.05 7.92 8.00 7.74 7.96 7.92 8.00 7.72 8.00 7.61 8.14 7~93 8.04 7.82 DO (malL) 7.8 77 7.8 7.7 7.9 7.9 7.9 .73 7.9 6.7 7.8 7.8 7.9 7.8 21.4% Conductivity (amlaoslem) 284 . 29'l 278 ~ 294. 280 ~.7. 286 298 ~
____________ Temperature ('C) 25.0 24.3 25.0 24.5 25.0 . 24.6 24.9 24.6 25.1 24.6 24.9 . 24.6 25.0 24.7 8.05 792 800 7.74 7.97 7.91 801 770 801 753 815 792 804 778 42.8% DO(EII~IL) 7.8 77 78 7.6 7.9 7.9 79 74 79 60 79 79 79 78 Conductivity (jembos/em) 257 26~ . .j~ 251 , 26(j 260 275 ~
_________ 25.0 244 251 24.2 25.0 24.4 250 247 251 246 249 244 250 246 pH(SU) . 8.05 795 801 7.75 '7.98 7.89 802 765 801 754 815 792 806 779 IDO (maJI.) 7.8 7.7 7.9 7.51 7.91 7.91 7.9 7.3 It i llmfl fkMIEIRI I . c.nI .~ Al~~J I85.6% Il*flHIIH*IIVIrV
]Temperature (°C) r" __ ___
IpU (S'U)_
IDO (meILl 4-Conductivity (atmhos/cm*
100% ' Akalinity (mglL CaCO 3) __
arn,*-ess (ma/L CaCO 3)
'To~taL Residual Chlo~rin (malL)
Temner*hle* (o[-* 25.0 24.4 25.3 24.4 25.2 . 24.4 25.2 24.5 25.1 24.3
____________ -, 4 - 8.02 7.77 7.97 .7.71 n.TI (Q'I'] 8.08 7.96 8.00 7.84 8.00 7.92 JI--..---
DO (ma/L) 7.8 7.8 8.0 7.6 . 8.0 7.9 7.9 7.3 7.8 6.8 Codctvty (junhos/em I 174 . . 178 177 187 180 100% Intake Alkalinity (mg/L CaCO*) 69 . 71 73
[Hardness (ing/L CaCO 3) .it------- 61 . . 65 69 hlrn
.iua fm Il ~0.10 - ., <0.10 . <0.10
. .tl .
Temnerature ('Cl 25.0 24.6 25.2 24.5 25.1 24.5 25.1 24.5 25.1 24.6
'Note: Total residual chlorine'was performed on non-treated Outfall 101 and Intake samnples.
File: sqnlOlj_120l5chem-PUV.xlsx Entered by:" J. Sujnner Reviewed by.
PagejLof ~
'1rotal Residual Chliorine (ORI0N-1977)
Matrix: Water,RLL= 0.10 mg/L Meter: Accunmet Model AR25 pH/Ion Meter
- .!. Analyst __ __ __ _
' -Iodidereagent:
'i".. Date analyzed LO -. i- i Acid reagent 0.10 mug(L [ 1.00 mn ,/L II(sunnestedmV Change ranne = 26 to 30 mV* I Millivoits(mV) I0 "q*,*
- h *-3 'j , "-,
N,ote: For samples with a residual chlorine of'> 1.0 rag/L, the samples must be diluted to be within the calibration'range.
Laboratory¢controlstandard:
Reference standard True value (TV) Measured value (MV) %hRS = MV/ TV x '100 1 number (ragfL) (mg/L) (acceptable range 90 to 110%)
I st4*S.*'5 0.50 'O.,r> i ,, o5. yh7J Duplicate sample precision: ___,__,__,__*____"__ .
- number " " *. L) (acceptable range - ' .. .:%
4 Duplicate D 0 0 e.
- Sample measurements: __________________, _______
"'Sample S pIe'D. Sample "characteristics Reslidual chlorine
,Reagent Blank ... O. ooo'4*
'Ood3 CAC,.o,.V *o C~0*o *o .0ooo*2...
- 'o2o..O' O~~r c~e*-,~~ catou.* (o.oooQ1,,V Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboritory controlstandard:
Reference standard True value (TV) Measured value (MV) .% RS .- MV"J TV x .100 number (rag/I.) (rag/L) (,acceptable range = 90 to 110%)
.. *3
- f 0.50. 0.1 ,C-647& ,I°/
Reviewed by E[ IZ""Z*
'...- ,,!. ': ...,:*" : . Date reviewed L1* .7-,i*" ."J
.. -,,S -:-
SOP C8 - Exhibit C8.l, revision 01-01-13
- ii*of 98 I I I Ill II I I I I = , ,
Page
- Page __. of j I Solutions, Inc.
Total Residual Chlorine (OBJION-1977)
Matrix: Water, RL = 0.10 nig/L Meter: Acecumet Model AR25 pH/Ion Meter h
Analyst Date analyzed [ -5 ,
[.j.I 7 j-j.- °dide reagent: I*
Acid reagent:. LI iIZ.'iq ?t 0.10 mgfL [ 1.00 mg/I, I mV'Change I(suggested- idmee.". 26 to.30 mV) iiReference- -tandard umbe*)r,, ..... i[ *.
Millivolts (mV)' 4",t*Oj) _, $5*
[,IS**",
a6,-* o04.'* J--*- . .",;!*i.i.. -
Note:.Forsamples with a residual chlorine of > 1.0 mg/L, the s~amples must be diluted to be within the calib~ration range.
Laboratorycontrolstandard:
- r. Reference standard number True value (TV)'
(ragTL)
Measulredl'value (MV)
' (mgIL)
% RS. = :*' (TV*..,100 I(aeceptable range *9.0!to11'%)
Sample T Sample ID Sample characteristics [Residualm/chlo°rine %]D(eeta!:inN
%RI ... [. .+/-*.12J)~x .,-.....i
)s 00"...s
- ={(S number .*'.
Sj Duplicate " vtvt, -----.. '.-'*.,",
Sample measurements: ,,"":.. " '**'. :*
Re:.,a.. ent. Blank;.:..:. ;
S~ample. Sample IDSapl charuacPtestcs L ...: "!,!4q*li~e
___....:__ ___:______ _ ,CO*O*o,',f*..'
161 2A.*c, t ka, ' ",10F * "O Note: All samples were analyzed in excess of EPA recommended balding time (15 minutes) unless otherwise noited?*.)**L!'. ..;'
L r*aborato-y controlstandard: "":'"!):: '
Reference standard True value (TV) Measured value (MV) dR'S M. iTV ~0,0*
.%fi
! *number I(mg/L,) I(rag/L) (aceptab* range ;29O'to 110%)
! Date reviewedLL II 1
! SOP C.8 -Exhibit C8.1, revision 01-01-13 Page 62 of 98
Page ')___ __
Page
- of I I 0 a
e II Inc.
envunmeiuaiiasttna Sohatiuns, Ii'.. Total Residual Chlorine (ORION'1977)
Matrix: Water, RL"O .lI0mg!L Meter; Accumet Model AR25 pH/Ion Meter
- I. Anayst FWii6 Date analyzed "[ii, q,-* I 3,
I odide reagent. ((*I Acid reagent: *!7* *U (j7 II 0.10 mg/L " 1.00 mg/L mV Cha.ge j I.' Note: For samples with a residual chlorine of > 1.0 mag/L, the samples must be diluted to be within the calibration range.
Laborator con~trolstandard:,... " III I' Reference standard True value (TV) ". Measured value (MY) % RS = MV / TV x 100 number I(m _g/) ... (ng/I.) . (acceptable range = 90 to 110%)j S~u II)*
[..nmeSample*.*'.*lDpiameSple SamPle. chara~cteristics._
. _.. S*'/Residual
,, m/Lchlorine. %P ( )/fSDl] 0
~1 I Sample measurements:_______
Residual clijorine I
SapeSample ID "Samplecharacteristics Reagent Blank ...... *, o*c*l I,
I Ii I.
Note- All samples Were. analyzed in excess of EPA recommended, holding timle(15, minutes) unless otherwise noted.'
'I
- Laboratory controlstandard:. ,
Reference standard . True value (TV) IMeasured va~lue (MV) %RIS =MVI/TV x 100 I number , (rag/L) I(rag/L.) *(acceptable range = 90 to 110%)
II Date reviewed /L *.2 t I,I SOP C8 - Exhibit C8.1, revision 01-'01-13 Ij Page 63 of 98
Page ______
Page 1 jof ~
lng SvIutIonsIric.
Mkal*]iint (SM 232) ]B-11997)
VMatr-ix: Water, RL = 1.0 mg CaCOO/L Time__initiated.
Dii:
ate analyzed ll=**,D Titrate samples to pH =4.S S.u, and mrnulilierdetermination:
~1 -~ = = = = ==
Normality Normality (NY) of H2 S0 4
- cheek Begin End Total =(5 ml Na 2CO3 x 0.05)IE* fi(Nx 50000)1/100 ml sample standard ml ml = 0.25/E =Nr 500 rnnmhar 'aceeentable ranee = 0.0180 - 0.0220*
_Laboratorycontrolstandard:.. . ....
- Reference standasrd Trure value Sample Alkalinity (MV) % 11$- yv/TV .xIODO number (TV) *~volume .Begin End Total Multiplier. (rag CSCO3IL) (acceptable range 1Dupllcatesample preZ!on: : ........ init Sample Sample ID volum~e 1 egin
'_________._ (ml) ,ml j End ml Total Multiplier (ng CaCOa/) I (s-D)1l(S+D)P]}x00~
7 iijjjj.. j .ge.. =j. )
Matrix spike reccl~rv jReference standard Spike value Sample 0 r _______.....__
Spike alkalinity (A) 0-fl number (SY) volume Begin End Total Multiplier (rag CaCOs/L)
~ ~(mi ziac~OgL) (ml) ml, ml ml ___________
...:Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 !
(rag CaCOa/L) MV A A- B .(acceptable range (racotaiO- =75to 125%)
S* le measarements ......... ........ _____
Sample volume Begin End Total .Alkalinity Sample number Sample IDml _ml. ml ml "Multiplier (mg CaCO.*
m*** _____ 157 )qa 3'.3 - __... _
______.___ Os*.(t *r. ____ __ __ __
Reviewed by: j~j~~jJ
- Date reviewed~ %D-a~. IS Page64 oC698SOP
- Exhibit C6.l]. revision 01-G1:13-
r~gv ________
Page _______of______
1 ~~
FmdfD~1meflt3ITGS~SOIut~o~sIRc.
Matrix: Water, RLL = 1.0 mg CaCO3 IL
-Time i'tit Time completed Date analyzed [7. j
- Titrae *smples *o PH = 4.5 S.1LL 7Wan~f wnrnialitt, und n*ulthoio. dojmv*irndo
- '-Normality N..
ormality (Nv) of H1S0 4 -==pH Factor or Multiplier ..
Deionized Titrant" Begin End Total - (5 ml Na 2CO3 xt 0.O5)/E = (Nit 50)000)1100 ml sample water reference sizM~ard ml* ml = 0,25/E *Nit500
= 4.5* S.U. number "number ,, (acceptable range,= 0.0180 - 0.0220) _____________
efrence standar-d Tr..ue va*lue ..... Sample ....... .... A lalnity (MV % RtS --MV / TV it 100" Rfenumber (TV) volume Begin End Total IMultiplier (rag CaCO3 /L) (acceptable range (mngCaCOsIL) (ml) ml ml ml I = 90 to 110%)
Dnplicate Sample precision:"__*__ __ ............. ___
- eee**anadsievlenSample A.. lkalinity pk %(PA)
Sampler " (pb- volume Begin End Total Multiplier (gCClL (Sa .DO~)./(+)2110 nube Irgt a~L (ml) . ml ml , mi ____ _____ (cetbenne~1%
Dulct (mg 1a71/ MV D 73-____aep___lera__
M ari spike resovem i s:
SnampltavardumSpikgvalue SapleTSpike Alkalinity(A Sapenumber (Samp vlume Begin End Toa l Multiplier ( g C~a.COiL rn )
(i"ng Ca,,,L ml m l m
[tefer .e _*... f I2. -3*.Q*,*30 *3,6 Reviewed by: [ ADate reviewed: *' )i-.*. l&
SOP C6 - Exhibit C6.1I. revision 01-0]- 13
Page
- of *
~,
Em~mJT0t1i.gS~Evons~Jn.
.Mk~Aini* (SM 2320 J*- 11997)
Matrix: Water, RL =1.0 mg CaCO3JL Time initiated Date analyzed [ , TIritrate sa*mpltCS to Time completed [7j7-* '7 pH *-4.$ SoU°T kreanirvl M, gujdml irnia dn jad dulnlo*rntbd Deinizd NrmaityNormality (N) of H2S0 4 p H Factor or Multip~lier eined Titrant ec.k Begini End Total =(5nl Na2 CO x 0.05)/E *(NxS50000)/100 ml sample water, reference stan* ml ml ml =0.25/B =Ni 500
--4.5 S.U. number number ** J L) ..(aereptable range - 0.0:180 - 0.0220) _____________
-- eeeneestaudard "Truevalue Sample .. .......... . . .. Am~lkaia ( %*MV+)S V/* 100 number (TV) volume Begin End Total Multiplier (mg CaCOsJL) (acceptable range (rg caC*/IL) ) ml .1 ml. ml . .-90 to110%) ,*.
Sample
- Sample ID volume IBegin End ITotal IMultiplier (mg CaCOg/L) + ((S -D) I[(S+D)12]~} z 100 Dl .& (a5:eeptcaboe rSmule 10%)
Reeence standard* Spike value Sample Spike alkalinity (A) number " (SV) volume Begin End Total Multiplier *(mg CaCOjIL)
- I (mngCaCOs/L) (ml) ml ml ml Sam+le measurements: ____...._
....... Smpivolume "Begin Enmd T...*otail ........ .. Aikainity Sample number Sample I'D .(mr} ml .ml mlI Multiplier - maCaCO/L) amo+, e.oj __"_"_____
Reviewed by:I Date reviewed: [- ! .**.f SOP C6 - Exhibit.C6. 1.revision 01-01 -13 Page 66 of 98~
Page ~
Page SD!UUgnsIr~c.
EnvimonlentolTeitblg Ailkaifnit (aM 232013°4997)
Matrix: Water, RL 1.0 nig CaCO /li3
[7~U77~7~~ Time ini*I7
-~ Analyst Time completed [I-I~I Date analyzed ~ Titrate samp~es t*o SpH = 405 S.U, lWwrnt nawnvnlhn nnoA 11inlhi~ ,lfADflNIflOfBflP H oxf -Normality -Normality (N) of YJI2SO4 pH Factor or Multiplier Deion*e at check Begin End Total = (5 ml Na2CO3 x 0.05)/B (N x 50900)11I00 ml sample
- water .reference -l~~drd niml ml ml fi0.25/B --N x 500
= 4.5 S.U. number numberr" ___ J*) (acceptable range = 0.0180 - 0.0220) ____________
Laborago controlrtandard: *______________________
efrence standard True value Sample .JAlkalinity (MV) % ItS =MV/TV x 100 number . volume. B*egin End Total Multiplier (uag CaGOs/L) (acceptable range (nig CaCOa/L) (ml) ml .ml ml ""- 90 to 110%)
Di~lcate-sa ~le recisoia:....... S m l [... j ' . . ..A k lnt [
- 0 -..... .
Sample SampleZHI volumie B egin 1E*nd [Total }Multiplier (mg CaUOsIL) R-D) /[(SD)/2]} il00
{(
number ml _____m____ ml____l__a_______e_____=___0%
Matrix spike recover, : ._______________________
Rieference standard ...spike value Sape......Spik alkalinityi (A*)...".....
numbe~r (SV) volume Begin End Total Multiplier (rag CaCO3/L)
(rag CaCO3IL)...mi ml ml ml II Sample alkalinity (B) ' .. Measured spike value (MV) %R
% = 1*/-SV lo0 (rag CaCO3IL) MV = A - B (acceptable range
.,(mg CaCOsIL) =75 to 125%)
Samnple measurements: ._*_ ____......
- Saimple volume Begin En*.d iTotal .. ' j Alkli~ni ....
Sample number Sample ID '(ml) mE sl m:nl Multiplier [ (rag CaCOs/L)
_ _ _ _* _ , ~ ._
___. ____ os* =
Reviewed by: Date reviewed: Tl0-.t, S SOP C6 - xhibit C6.1. revision 01-01-13 Page 67 of 98
a Page ______
Page
~ whunmentalTesttng5o!uklons.Inc.
AllaInity (SM X32A) B-31997)
Matrix: Water, RlL =1.0mg CaCO3 IL Time initiate*. 777[
Analyst Date analyzed O i i* " i Time completed ]I I .I~iII Titrate *am~p]an to pH = 4.5 S0IL.
Dnplicatesa* pe preeison: ______ _________
sample Sample XD. volume IBegin ]nd E Total .Multiplier (migCaCOsg) (('D){ J(S.-D*ti")/*]} ilU number_ __ _ .__ (ml) .ini ml ml _ ___g_
Mfatr~i spike recovery: ...... __________________.__
IIReference standard Spike value Sample .... ..... Spik~e alalinity (A) ......
number (SV) .volume Begin End Total Multiplier (ing CaCO*/L)
(mug CaCOs/L) (ml ml ml nil..
(rag CaCOzIL)
S(mag MV = A - B CaCOs/L)
(acceptable range
=75 to 125%)
A Sample measuremenis: .............. .. __ ____________
Sample volume ... Begin End Total Alkalinity Sample number Sample ID _.(ml) _ ml ml ml Multiplier (ma CaCO3 /L)
- o*.* ,* *_! __ ,*.Q *,j S. _,__ .
Reviewed by: Datereviewed: [, b.1.ISL SOP C6 - Exhibit C6.1. revision 01-01-13 Page 68 of 98
.Page 7 0
~ E'~vIr~nment3~Te5tIng Solutloni, Inc.
. Hiardoess (SM 2340 C-i997)
RL = 1.0 mg CaCO /L 3 Analyst[ j7* Time initiated **
Date analyzed [ q* Time completed [*'*-jj Thrant ,*ormality and ,wdtplier detrminaio:
Ti-trant ... Normalitycheck£ Begin Endc Total ... Normality (J) of EDTA ...... pH D'actor or Mul'tiplier reference standard ml ml nml-- 0.2/15 = (N x 50000)/S0 sal sample.
number number .. (E) (acceptable range = 0.0180 - 0.0220) = N
- 100O Lasboratory control standard: ,__________,_________
1'apl'e
- Reference standard number f True value (TV)
(sag CaCOa!L) volume (mu)
Begin m
End ml Total ml 1Multiplier J=
Hardness (MV)
(rag CaCO.IL)
%RSMI*-= VTV 21@0 I (acceptable range 9Otol110%)
Duplicatesam *le precision: .
--- ' Sample . H..ardness.. .... % * :.....
Sample Sample ¶0 volume Begin End Total Multiplier (rag CaCOs-L) {(S -0 /1)(,541)121) x 100 number. ______ _ ml)~ nml ml nil ___
Matrix spike recovery: _________________________________
Reference standard Spike value Sample ... ...... Spike h~ardness ('A) number (SV) voumne Begin End Tota ul~tniplier (nig CaCO3 IL)
(ag CaC.O.3L) inl ni l ml Sample hardness (B) 'M'asured'spike value (MV%) ' % Il MVgfg/SV x 21008 (mug CaCO3 IL) ]V = A - B M (acceptable ranlge I (n.i g CaCO3 I.). = 75 to 125%)
Sample number Sample 110 (m1m ml ml M~ultiplier (rag C.aCO3 IL)
TV'ND(should beo= O.rg CaCOs/L) __
Jb°*"i * ' : .O ___ *7 !*O tl _ __ _ __
I Reviewed by: Date reviewed [ 4 Note: If >1 5m1 oftiirant is used, sample must be diluted.
Page 6C7of 98SOP
- Exhibit C7.1. revision 0 1-01-13
a Page
- I Page _* ~of 3 I bnmn~a1TesUngS~dut~wi~ Inc.
lHardness (SM 2340 C-31997)
RL =1.0 tug CaCO3/L Time initiated .
Analst Date analyzed Time completed [I I[I F
I?
Laborot ca Reference standardo _t lstandard:
. Tr'e value.......
Sample :. ...._ __ ......
H.. _
-ardness __ _
(IWV) __ __
"...%R _ __
='f ....
MVJ:/TV_ __
. 100 _
number (TV) volume Begin End Total Multiplier (inag CaCOjIL) (acceptable range
________(rag CaCOML) ( ml, m ml ml .. = 90 to'l10%)
D~ lcatesampleprecsion: .... . . .. . .. . .*-..... ... .,.: -
.... " .......... I Sam ple . . . .. .] Ha rdness. ..- "i _'* " .... .'
Sample SampleZ ]Tvolume Begin *nd ,Total Multiplier E (mg CaCOsFL) {(S "- .*9$0, number ______ .*L l m l___
ii Matrix spIke recovery , ,, , ___________
Reference standard 'Spike valiue Sample Spike hardness (A) number . (SV) volume Begin E.nd Total Multiplier ,(mg CaCO3dL)
_______O__ (mi) m!l~jL m l4
,Sample hardness (B) Measured spike value (MV) '% R -MV!SY xi100
! (nmg CaCOsIL) MV* ffA - B (acceptable range Sample volume Begin Egnd Total Hardness Sample number Sample ID (ml) ml *ml ml Multiplier (ma CaCOsJ)
TV ~ (should be-" 0 mg ______ - 4 -- 2*h*
D*I leO'1* -* __ ___ ___ - o- ').* o.
, *
- _.____, , i* ., _ __.._.. _ _ _ _
Note: If >15rml of titrant is nsed, sample must be diluted. -Reviewed by: I
- iiI Date reviewedI [o~do*
SOP C7- Exhbit C7,I. revision 01-01-13 Page 70 of 98
Page I*
Page -* of~
4~ I.wimIment3IT~stInD 5DtutIon5dn~.
JHlardmess (SM 234~O C-11997)
RL.= 1.0mg CaCO 3/L~
Time initiated * " 12 Date analyzed Time completed [I I* I Tk, antnormalit andmutiplier determination:*............
.... *-~rnt* * " otl Nornmlity (N*)of FDTA pH Factor or Multiplier refeencrn stndadml * " ----. *. _ _ * .... =(Nx 50000)!/50 mi sample number number CE) (acceptable range=.= Nix 100 Labondorj'contlvol standard: ________________________
eerence standard 3ue value Tr. ... Sa[ieHardness (MV- %1S = MV! FT x 100 number - (TV) volume Begin End Total Multiplier (mag CaCOsIL) (acceptable range
._Dup_ licatesam21e preciiOn: _______________
Sample . .. :* .. *Ha,r-dn-ess .......... %RPD = "....
BSample Sample ID volume Begin Ed.oa utpir(a aOL (-)[SD/] 0 number m). md
- n E*b** ta*l ( - "utpir(igCCsL
.)I(4D~)xil Matrix spike recovery __________"_________"____
.Reference standard Spike value Sample .... .. ... ... spike hardness (A) number (SV') volume Begin End Total Multiplier (ag CaC*sL)
' CaCOa/L)
(mg}*O* (m) nml ml ml (mgpl CaCOS/L) Meaue = A-B (acept~able range
-.. (sa CaCOs)L = st 125%)
S mle measurements:________
- Sample volume "Begin End' Total' Har.....
dness Sample number Sample lID fml ml ml ml Multiplier (mag C.aCO.3L)
TV ___- -... = - *
- _ - -3.*, *.* __ ___-
Note: If >15m1 of titrant is used, sample must hediluted. Reviewed by: [
- Date reviewed [ *4i6*.
SOP C7 - Exhi'bit C7. I. revision 01-01-13 Page 71 of 98
Sequoyah Nuclear Plant Biomonitoring October 20 - 27, 2015 Appendix D Reference Toxicant Test and Control Chart Page 72 of 98
- Pimephales promelas
@ ~Chronic Reference Toxicant *Control Chart
- *'* *Organism Source: Aquatox, Inc.
S Environmental Testing Solutions, Inc.
I.,o I I I I I I I l ! J I I l I ! J ,
i *PP* * -! - .
II .. L -__ It. = q __ _--_.--- -] I _ _*B. .
UM:VA Lonltrol LimitS *__L :tanaaro ueviatlons) 0,9 0,8 0.7 0.6 0,5 I I I I 1 I I I I I I I I 1.4 S I ] I 1 I I I I l I I ! I I I ' I USEPA Warning and Control *Limits, (75t and 90t Percentile CVs) 1.2 ..................................... *
- 1.
i>.0.6 "U L A 0.,4 1 I 1 .1 I I I I I I I I I I I I I I I I I 1.4 i J L I i I l Ii II I I I I i I I I I I Laboratory Warning and Control Limits'(10h and 25h Percentile CVs) 1.2 1.0 0.8 0.6 0.4 I I 1 I I I I I I I 1* I I I I I I I I I Test date
7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- Central Tendency (mean IC2 5 )
- -* Warning Limits (mean IC2 5 :+/- SA.10 or SA.75) Grahsg....ted frm ... soeited .... 1, spreadshaat.
- % .. .=:,'" Control Lim its (mean IL2 5 -+SA 2 ,S .0 or 2 Standard Deviations) Excelspreadsheetente dbv: l.Sum nrr aAA~5 ~.9'Reviewed by: *l 73 of 98
rsE Pimepholes promelos Chronic ReferenceToxicant Control Chart i-'** Environmental Testing Solution s, Inc.
0 Laboratory" Laboratory USEPA ' USEPA Stats and USEPA 5 Test number Test date 7-day lC* CT S Control Umits 5** Warning Unmits S*J Control Umetts $As5 Warning Urnits naas Control Uimits CV WIJ KcC) (g,/LCC) Cr-2s a+2s CT"-Sku CT+ u, r-5*s~ aT 5s2 aT-S41 s aT+~s5 a'"- 8 8 , C" 5*s..*,
I. 07-08-14 0.74 0,76 0.05 0.67 0.85 0.09 0.67 0.86 0.16 0.60 0.92 0.29 0.47 Los 0.34 0.42 Li10 0.06 2 08-05-14 0.88 0.76 0.05 0.66 0,86 0.09 0.67 0.88 0.16 0.60 0.93 0.29 0.47 1.06 0.54 0.42 Lii1 0.07 3 08-12-14 0.82 0.77 0.05 0.67 0.87 0.09 0.68 0.89 0.16 0.61 0.93 0.29 0.48 1.06 0.35 0.42 £12 0.07 4 "09-09-14 0.81 0.77 0.05 0.67 0.87 0.09 0.68 0.87 0.16 0.61 0.54 0.29 0,48 £07 0.35 0.48 L.12 0.07 5 10-07-14 0.77 0.78 0.05 0.69 0.87 0.09 0.68 0.87 0.16 0.61 0.94 0.30 0.48 1.07 0.35 0.43 1.13 0.09 8 11-04-14 0.82 0.78 0.05 0.89 0.87 0.09 0.68 0.87 0.16 0.61 0.94 0.90 0,48 L07 0.35 0,43 £13 0.06 7 12-02-14 0.74 0.78 0.04 0,69 0.87 0.05 0.69 0.87 0.16 0.61 0.94 0.30 0.AS 2.07 ,0.35 0.43 1.13 0.06 8 o1-1a;!5 0.76 0.78 0.04 0.70. "0.88 0.09 0.69 0.87 0.16 0.62 0.94 0.30 0.48 1.09 0.35 "0,43 £.13 0.05 a 01.-21-15 0.81 0.78 0.04 0.70 "" 0.87 0.08 0.69 0.88 0.18 0.82 0.55 0.30 0.49 £0O8 0.35 0.A3 £.13 0.05 10 02-03-15 0.74 0.78 0.04 0.69 0.87 0.09 0.69 0.87 0.16 0.62 0.94 0.30 0.48 1.08 0.35 0.48 L,15 0.05 11 02-03-15 0.78 0.78 0.04 0.89 0.86 0.09 0.69 0.87 0.16 0.61 0.94 0.30 0.48 1.07 0.35 0.43 LIB3 0.05 04-07 -15 0-st 0.75 0.04 0.71 - 0.88 0.09 0.65 0.58 0.16 0.62 0.95 0.50 0G4S 0.55 0.43 L.14 0.05 13 05-05-1.5 0.81 0.78 0.04 0.71 0.86 0.09 0.69 0.88 ="0.16 0.62 0.55 0.30 0.48 L08 0.355 0.43 L.13 0.05 14 06-09-15 0.82 0.78 0.04 0.71 0.86 0.09 0.89 0.88 0.18 0,62 0.95 0.30 0GAS 1.08 0.85 0.48 1.14 0.05 15 07-14-15 0.78 0.78 0.04 0.71 "0.86 0.09 0.69 0.88 0.26 0.62 0-95 0.30 0.49 Los 0.35 "0.43 £.14 0.05 16 08-04-15 0.83 0,79 0.04 0.71. 0.85 0.09 0.69 0.88 0.16 0.62 0.95 0.30 GAS9 1.08 0,35 0.43 £14 0.05 17 08-11-15 0.91 0.79 0.08 0.72 0.88 0.09 0.70 0.89 0.17 0.62 0-86 0.30 0.AS £09 0.366 0.43 1.15 0.04 18 05-1_5-15 0.81 0.79 0.03 0.75 0.86 0.10 " 0.70 0.89 0.17 0.63 0.96 0.80 0.49 1.09 0.36 0.44 £15 0.04 19 20-09-15 0.78 0.79 0.08 0.73 0.88 0.10 0.70 0.99 0.17 0.63 0.96 0.30 0.49 L.09 0.38 0.44 £15 0.04 20 10-20-15 0.72 0.79 0.04 0.72 0.88 0.09 0-70 0.88 0.17 0.62 0.98 0.30 0.A9 LOS 0.36 0.49 1.15 0.05 Note:- 7-d lCzs 7"-day28% inhibition concentration. An estimation of the concentra tion of potassiuam chloride that would oause a 25% redaction inlPirephales growth for the test population.
CF"= Central tendency (bean 1%~).
0 S = Standard deviation of the I ssvalues.
Laborato~r Control and Warning Umits Laboratory control and warning limits were established using the standard deviation of the [1%winues corrasponcin8 to the 10th and 25th percentile CVs. Tlhese ranges are t~norestringent than the control and warnieg limits recommended by USEPAfor the test method and endpoint.
5 SLio = Standard deviation corresponding to the 10dhpercentile CV. (5,A,,= 0.12) 5z= Standard deviation corresponding to the 2?t percentile CV. )SA~S = 0.21)
USEPAContmol and Warning Limits 5
a~rs=Standard deviation corresponding to the 75th percentile CV. ( *7 0.98) 5 ea= Standard deviation corresponding to the 906*percentile CV. (8 o0.45)
CVg = Coelticionr oolvariation o*tie ICrs vaiues.
USErA.250.0Ulndestandlng*and Peliutant olsdiarge Elmienslton is Whole EffluentTenidtv Appllradnns Underihe Slatinnal AzcsustinglferlMethod Variability USEnvironmental Protection Agency,Oncinnati, Program. EpA-833-R-t0-053. OH.
File: ppskntj0_*20l5.nisx CEtered by, J. Sunorer Reviewed by.
Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements SE nvironmental Testing Solutions, Inc. Organism Source: Aquatox, Inc.
1.25 - I I I I "II I I I .I I I 1 .
S1.00 2-_ 0.75
.0 b*
E 0.25
-USEPA Acceptance Criteria (>0,25 mg per surviving larvae).,
- II I I I I I I
- i I I I I I I i I I I 30*
Kentucky AccePtance Limit (< 30.0%)
(UO I-201 wW
"-2 04-a 4.' *10 w 0 aD 0
- I I I I I I I I I I I I I I I I I I I I -
30 I, III'.I I I I I I I I I -
- USEPA Upper PMSD Bound (90t percentile < 30.0%)
20 1O mj O.DD*
lO* ea ~ ~ *O **e
- P*I O O* *IO
- I I I I I I I I I I I I - I I I I I. I I 0%
Test date
--- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference*
(PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
~Central Tendency (mean Control Growth, CV, or PMSD).
SControl Limits (mean Control Growth, CV, or PMSD + 2 Standard Deviations)
Grphsgeeein~ted from associated aecelspreadalheet.
Excelspreadsheet entered by: J. Sumner
- -Page 75 of 98** Reviewed by: %.
- IT Precision of Endpoint Measurements 0
.. Pimephalespromelas EnvlronimentalTestlnysoiutiorss, Inc. Chronic Reference Toxicant Data Test number Test date Control sria Control Mean Growth cr CV CF" MSD PMSD Cr"
(%)
%) (g/lavae)
(ag/lavae), for Control Growth (mag/larvae)
(96 for Control Growth CV (%) (96) forPMSD(%)
- 1 07-08-14 100 0.631 8.3 0.06 9.0 2 08-05-14 100 0.560 0.596 9.0 8.6 0.07 13.3 11.1 3 08-12-14 100 0.694 *0.628 2.6 6.6 0.11 15.6 12.6 4 09-09-14 100 0.622 0.627 11.7 7.9 0.10 .15.7 13.4" 5 10-07-14 ioa 0.617 0.625 11.6 .8.6 0.11 17.2 14.2 6 11-04-14. 100 0.627 0.625 5.6 8.1 0.05 8.6 13.2 7 12-02-14 100 0.712 0.638 8.2 8.1 0.09 12.6 13.1 8 01-13-15 100 0.551 0.627 8.7 8.2 0.06 10.8 12.8 9 0-1-21-15 100 0.540 0.617 .10.9 8.5 0.08 14.7 13.1 02-03-15 100 0.585 0.614 13.7 9.0 0.08 13.5 13.1 11 02-03-15 100 0.607 0.613 5.5 8.7 0.07 12.2 13.0 12 04-07-15 100 0.520 0.606 4.3 '8.3 0.06 12.1 12.9 13 05-05-15 "97.5 0.536 *0.600 13.0 8.7 0.07 12.7 12.9 14 06-09-15 100 0.534 0.595 2.5 8.2 0.05 10.2 12.7 15 07-14-15 100 0.556 0.593 4.6 8.0 0.10 17.5 13.0 16 08-04-15 100 0.539 0.590 11.9 8.3 0.07 12.1 13.0 17 08-11-15 100 0.600 0.590 7.8 8.2 0.06 9.7 12.8 18 09-15-15 100 0.545. 0.588 8.7 8.3 0.06 11.1 12".7 19 10-06-15 100 0.590 *0.588 6.2 8.1. 0.07 11.7 12.6 20 10-20-15 100 0.584 0.588 10.6 8.3 0.08 13.9 12.7 Note: CV = Coefficient of variation for control growth. "
Lower CV bound determined by USEPA (1 0 thpercentile) =3.5%.
Upper CV bound determined~by USEPA (90"' percentile)= 20%
MSD = Minimum Significant Difference PMSD = Percent Mlninmum.Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatnent th~at can be declared statistically significant In a whole effiuent toxicity test.
Lower PMSD bound determined by USEPA (1 0 Th percentile) = 12%.
a'-
Upper PMSD bound determined by USEPA (90 percentile) = 30%.
CT =Central Tendency (mean Control Growth, CV, or PMSD}
USEPA. 2000. Understanding and Accounting for Method.Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH. -
.USEPA,. 2001a, 2001b. Final Report: Intedlaboretory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2 Appendix. EPA-821-R-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
-PgeEntered by: J: Sumner Pae76 of 98 Reviewed by:_ _
0 Page 1 of 6 a
Emnmo~InS~rutIonoIoo.
Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales p.romelas PpKCICR Test Number: 30_77 Dilution preparationinformation: Comments.:
KC] Stock INSS number: 1NSS ltV( _________________
Stock preparation: 50 g KCIIL:
Dissolve 50. a KCI in ILMl- water.. .____________________
Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mIL) 6 9 -- 12 15 18 21 .......
Diluent volume (niL) "994 '991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 "I0 00 Test organism information: __________ ...... Test information:
Organism age.: l*. %0*S. .. , Randomizing template: ______
Date and times organisms were 10-19-15 1600-1700 Incubator number and born between: shelf location:_______
Organism source: ATOX Batch Pp: 10{)-19-15 Artemia GUM number: CHM836 '
Drying informationfor weight
- .. determination.',______
Transfer vessel information: pH = "1PtO S.U. Date.].Time in oven:, *.iiotS 12.*
_ __ _ _ _ _ _ _ _ _ _ Temperature - "LA , °C Initial oven tem perature: 'tj ° .
Average transfer volume:' 0.1749 mL Date / Time out of oven: t..%t*
Final Oven temperature: ' C..
Dailyfeeding and renewal information:
- "Day Date Morning feeding Afternoon feeding' Test initiation, renewal," MHSW.
_____ ______________ or termination batch used
,Time Anal ,st Time Analyst Time Analyst . ............._
0 10-20-15 _______ " ,.* i,.*o 'O "so* *[ IU" 2 10-22-15 o*cK) u*[ lql * '3"': .. 1( I S2-1 6 10 15 7 10-27-15" lo~Ib~
Contrtol information:; .. . Acceptance, criteria,, Summar of test endpoints:
%M,. t ,'o1 _<20% 7-day LCso 50 ~ o*
Aver~age weight per surviving larvae: __ . >*..*
0.. mg/larvae
_.25 LeEC, ,_______
indo~.nd.nI ChV S
~o4o~o by IC2 5 ~
SOP AT21 - Exhibit AT21.1, revision 11-01-14 Page 77 of 98
Page 2 of 6 Species: Pimephales Dromelas PpKCICR Test Number: 307 Survival and Growth Data Day 0
F -
A 10 i
I -
B 10 Control I -
C 10,
~~
i D 0=
10 300 me KCI/L
~ -
F 10 I -
G I
~ -
Hi 10 0 -
U -
I Io
~
I 450 m* KCf/L J
10 K
10 L
10 10 10
- 10) '0 b0 *0 '0 b 10 I0 l0 & D i I
II
)& W i(0 10 10o (0 10 10. '0 10 to U
80 10 1010/ tO 1.0 10 10. 10 10 t0
/0 10 10 10I tO tO 10 10 tO" 10 10
/0 10 I0 101 1Q *t0 1O Tray color code:: I~l4 k*t*
A = Pan weight (nag) . '... .. ... . . .I "
Date: O~t- 2,4- I $""
B =Pan+*-La'vae weight (rag)
Analyst: 4 J/h **. *L3 *L *J*7
- V
- .1 *00 *S *P
- .*l.
Date: W'-13*
C=-Larvae Weight (mig) =B -A .
Analyst:, ,
Weight per initial number of larvae (mig)
-=C /Initial number of larvae
(¶1 Hand calculated.
Analyst... '
A, 1% £2 Average weight per initial Percent I -. 1 number of larvae (rag) ' reduction . e _
- I = = ~ 1 , =
- from control O5~ tAt
.Commentcodes: c clear, d --dead, fg tbfngus, k killed, m = missing, sk = sick, smn unusually small, ig = unusually large, dgr = deeaned and returned, w = 'wounded. .
e78 of 9 8 SOP AT2I - Exhibit AT2!.1, revision 11-01-14
0 Page 3 of 6 S
EnvJ t,,ITtIIgSdUIIWIS.IM.
Species: Pi'mephalespromelas PpKClCR Test Number: 30..7
_____ ____ ____ _ _______________Survival and Growth Data 750 mg KCIIL' 900 mg KCI/L Day . .... 600 mgKCi/L,_ __ _
_ _ _ _ _ _M N 0 P Q .R S T U V *W X 0 0 10 " 10 10 10 10 10 10 10 10 10 10
_________ __ o* to to *0 ut'
,o *
- o
- __ __ __ 10 " to Io tO \ q 4b '
a*-. *
'p0%0tItO 7 ..... \ o*i* *' .6%
A- Pan weight'(rng) -- - - - ' I Tray color code::i, I-~ 5o 5 I4 43I3i~ -
Anlst ___,_ ..
__o__.______.*2,,q,'t G.tB ,.1q i, I'~I0 15.A3 i*..*z ie.*,
D~ate: flG - .2,.-_.I ;.
B=Pan + Lairv~e Weight (rag)
Analyst: w Jb,. "* . i ,.o .,1 ,, ,,iq* i* 1" t l,. 3 ,3 1. ,S I.*
C = Larvae weight (rag) = B - A Analyst:
Weight per initial number of larvae (rag).
Hand calculated. ,. ***,t/*'
Analyst: * "* 0* *° * .* "0 Averageweight p'er iuiti"al Percent ' '
number of larvae'(mag), reduction )j*.
2.S7, OA%' 2EI&,
- Oo from control Comnment codes: c. clear, d =dead, fg = furngus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r --decanted and returned, w --wounded.
Cottments:
by VMI.yEK..n.m SOP AT21 - Exhibit AT21.1, revision 11-01-14 age 79 of 98
Page 4 of.6 Species: Pimeohales i~romelas PpKCICR Test Number: 30_.7.
I Comment codes:
Ig= unusually c =d&-r large, clear,= decanted d --dead, and fg =returned, fungus, k-=
w-=killed, m = missing, sk = sick, sm = unusually small, wounded.
b~
I,'- SOP AT21 Exhibit AT21.1, revision 11-01-14 Page 80 of 98 -
_a Pimepholespromekws Chronic Reference Toxicant Test 0
a *EPA-821-R-02-013, Method 1000.0
,I' to Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc.. Test number= 307 Test dates: Octeober20-27. 2015 Initaltnumbare, ler eyermoreS A=NawnwerhtCmii 6= Pam+-etaw lIt.. lktu~ms] W.Ile/dSwersig M ac hUten',edpt/* cooffkiaifenetvrcn r WozlutOe/lalnemharoiMa snusmleeli honwcrlgh/'l*-It MK udfileasraln Pnntnufefn Cin.in~ebtlen(nxj~l.Isj bptai.
Irm Imm Vrlll~ml AB uma~f~*CL* um~clemvme bmnms.Ntii * .~* Inun off
- zl l A 10 10 16.16 22.13 5.97 0.597 .0.367
- 6 " 10 10 16.57 21.50 4.93 0493 0.8 060.461100 0541.6 Ntapial Control C ... 10 " 10 15.94 22.16 £352 0.632 - .63J2 100 0591. oeola
- E 10 10 16.55 22.29 5.74 0.--/4 0.574 P0 10 10 16.72 22,45 .5.73 0.573 "0.57310. 0.29288 0 10 10 16.41 21.21 4.80 0.460 - 0.460
- _______ II 1 10 15.07 20.03 5.01 0.501 ________ 0.501 __________ ______
I 10 10 16.14 21.05 4.91 0410.491 450 J 10 10 - 15,49 20.86 5.37 0.537 0.537 100 094701.
C :10 10 16.24 21.76 3.52 0.552 .4700,552
- L 10 10 £4.81 19.56 4.77. 0.477 ________ 0,47-7 _____ ______
M 10 9 15.09 19.39 4.30 0AT67. 0.430 60NP 1o 9 16.86 2.1.62 4.76 0.529 0466 10.7 0.476 9. .6 062.
60 o . o 0 15.52 21.22 5.30 0.530 "0.510 6. .6 062.
P 1 10 15.42 19.62 4.20 0.4251 0A420o ____ ____________
O 10 9 14.43 19.4.5 5.02 0.556 0.502 750 5 .. 10 B 15.43 19.42 1.99 0.496 0.39615259.3 1. 2.
S . 10 7 13.91 17.91 4.00 0.571 0,400
_____"__ 10 9 14.42 15.66 4.24, 0.471 -0.424 *______ ______
U 10 7 1450 18.33 -3.43 0.490 0"343 W 10 6 16.20 18.59 3..39 0.565 - 036 - 650.335 50 030554.
X "10 7 15.55 19.72 3.77 03519 . .377 _ ___ ______ ______
1' 10 3 16.18 17.60 1.42 0.473 0.142 120 10, .. 1 16.57 17.41 1.04 1.040 ,7 570.104 20.0 0.129 14.5 78.0 LA 10 2 16.06 " 17.32 1.24 0.620 0..1.133.7
_________ 6 10 2 16.22 1i7.66 1.44 0.720 _______ _______ 0.144 _____________ ________
Dunneetu' MSOvalue: 0.0811 MIVD'= Minimum S]gn~fcant Difference P635D: 13.6 PMSrO = Percent Minintum Significant Difference PMSD is a measu;re of lest p recision. The PN145 isthe minimum percent dlffereeee between the esetrol an troaltmentlthat can be deciared statistically significant In a whole effluent tosicity teet.
Lower PMSD bouuid determined by USEPA (10th percentile) m12%.
Upper PMlSDbound determined by UIIPA (90th perceetite) =3014.
iLower and upper PMSD bounds were determined from the +/-0th 3rd 60th pe rcentlie, mapectively. of PMSD0data from EPA's WET ieterln boralory VeriahilityStudy (USOPA, 2501,5; UIEPA, 2001b).
USEI'A. 200oa, 2001b. Finel Report: Interleber'utory Variebilityltudy of EPASIhort-terrnvChronlc and Acute Whole Effhuent Tonicity Test Methods, Volumes leand 2-Appendie. EPA-821-6-01-.004 and EPA-821-5-01-O05. US Environmental Proteetion Ageecy. Clnclinnati, OH.
P*ile: ppicci-tr 0Z01S.clsx Reviewed by: *f
tonmoJt*IThatn~SaIw1on,,
I~.
Lerval Fish Growth and Survival Test-7 Dae Survival Start Date: 1O/2012015 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sal. Sample Type: KCL-Potassium chiloide Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephsles prameias Comments:
Conc..mglL 1 2 3 4
- D.Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000. 1.0000 480 1.0000 1.0000 1.0000 1.0000 600 0.9000 0.9000 1.0000 1.0000 750 0.90003 0.8000 0.7000 0.9000 900 0.7000 0.6000 0.6000 0.7000 1050 0.3000 0.1000 0.2000 0.2000 Transform: Arasin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-.Mean Mean Mmn Max CV% N Sum Critical Reap Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 "0 40
.300 1.0000 1.0000 1.4,120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 460 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9500 0.6500 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 40
- 750 0.8260 0.8280 1.1491 0.9912 1.2490 10.866 4 10.00 10.00 7 40 "900 0.6500 0.6500 0.9356 0.8861 0.9912 6.464 4 10.00 10.00 14 40 "1050 0.2000 0.2000 0.4572 0.3218 0.5796 23.087- 4 10.00 10.00 32 40 Auxiliary Teats Statistic Critical Skew. Kurt Shapiro-WIli's Test indicates nlorrnaldistribution (p > 0.01) 0.91641. 0.869 -0.369 0.51366 Equality of variance cannot be confirmed Hypothesis Teat (1-tell, 0.08) NOEC LOEC ChV TU Steels Many-One Rank Test 600 7850 670.82 Treatments vs D-Control Maximum Llkellhoodi-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical *P-value Mu . Sigma iter Slope 10.3756 1.56043 7.31719 13.4341 0 3.9497 9.48773 0.41286 2.96401 0.09638 3 Intercept -25.753 4.58766 -34.745 -16.762 TSCR 1.0-Point Probits mgIL 95% Fiducial Limits 0./
ECO1 2.674 549.286 449.219 615.4890.
EC06 3.386 638.971 554.374 694.609 o.8 EC10 3.718 692.621 619.065 742.166 0.7 EC15 3.964 731.342 668.988 777.169 EC20 EC25 EC40 4.158. 763.653 704.855 607.246 4.326 792.509 738.968 838161 4.747 870.15 824.957 918.041 ap0.6 o.s1 04 EC50 5.000 920.474 874.807 979.405 04 .
- EC60 5.253 973.709 922.5 1050.25 0.3 '
EC76 5.674 1089.1 1000.91 1167.850.
ECS0 5.842 1109.5 1032.4 1249.090.
EC85 6.038 1156.52 1069.72 1326.26 0.1 ECO0 6.282 1223.28 1117.88 1428.62 0.0 EC95 6.645 1326 1192.28 1598.19 1 10 100 "1000 180000 EC99 7.326 1542.8 1343.42 1978.43 Dose mglL Dose-Response Plot 0.9i 0.81 o.61
- 0.4i 0.2' 0.1i Filc ppkclcr_102015.sxl Entel:redby: J. Sh*mern Reviewed by: __*s~
82 of 98
9 0
0 C
Statistical Analyses tnvtronm.isniintieasetnntitte.
Larval Fish Growth end Survival Test-7 Day Growth Sample ID: REF-Ref Toxicant Start Date:
End Date: 1012012015 10J27/2015 Test ID: PpKCICR Lab ID: ETS-Envi rt. Testing Sot. Sample Type: KCL-P~otahsium chloride Sample Date: Protocol: FVVCHR-i SPA-521-R-02-013 Test Species: PP-Pimephalea pronmelas Comments:
Conc-mgIL 1 2 3 4 D-Control 0.5970 0.4930 0.6320 0.6120 300 0.5740 0.5730 0.4800 0.5010 450 0.4910 0.5370 0.5520 0.4770 600 0,4300 0,4760 0.5300 0.4200 750 0.5020 0.3990 0.4000 0.4240 900 0.3430 0.3350 0.3390 .0.3770 1050 0.1420 0.1040. 0.1t240 0.1440 I-Tailed Isotonic Conc-mgJL Mean N-Mean Moan ... Transform:
Min... Max Untransformed CV% N t.Stat Critical MWD Mean N-Mean D-Control 0.5835 1.0000 0.5835 0.4930 0.6320 10.628 4 0.5835 1.0000 300 0.5320 0.9117 0).5320 0.4800 0.5740 9.151 4 1.464 '2.290 0.0811 0.5320 0.91 17 450 1.0.5143 0.8813 0.5143 0.4770 0.5520 6.986 4 1.955 2.290 0.0611 0.5143 0.8813
- 600 0.4640 0.7952 0.4640 0.4200 0.5300 10.842 4 S3.374 2.290 0.0811 0.4640 .0.7952 750 0.4313 0.7391 0.4313 0.3990 0.5020 11.281 4 0.4313 0.7391 900 0.3485 0.5973 0.3485 0.3350 0.3770 5.532 4 0.3485 0.5973 1050 0.1285 0.2202 0.1285 . 0.1040 0.1440 14.510 4
- 0.1285 0.2202 Auxiliary Tests . .Statistic Critical Skew Kurt Shepiro-Wilk's Test indicates normel distribution (p >' 0.01) 0.93919 0.84.4 -0.3921 -0.8388 Bartlett's Test indicates equal variances (p = 0.88), 0.75495 11.3449
,H.y~pothesis Test (1-tail, 0.08) NOEC LONG ChiV . TU MSDu MSDp 1535 155 F-Prob .df Ounnati's Test 450 600 519.615 0.06111 0.13901 0.00973 0.00251 0.0377 3, 12.
Treatments vs D-Control. . . ... , .
Point malL SD 95% CLIExp,) Skew ICo5° 169.95 115.20 .31.34 848.89 0.9788 10[10 357.89 123.81 51.89 702.17 0.2743 0C15 804.55 109.99 97.20 782.28 -0.2747 1.0o 1C20 591.64 92.60 3990.18 "568.07 0.4122 .
1025 720.80 53.65 453.08 870.42 -0.3573 a 1040 827.10 23.73 802.35 939.69 -0.5913. 0.8 1C50 938.89 10.17 909.28 970.18 0.0687 .
- indicates IC estimate less than "thelowest concentration*
0.4 0.3 0.2 0.1 0.0 , ,
500 1000 1590 Dose mgIL Dose-Response Plot 1-tail, 0.05 level of significance 1 0.4' C.3.
0.2 IDdQtfldSflt enliw by Kelleyt.K*eeae:
Pile: ppklcdrj102OIS Enteredby: .xlsx 5.Spiner Reviewed by:.~I Page 83 of 98
Page 5 of 6 nwenxaITe,,gsor~IoniI~,,.
Species: Pimephalesvromelas PpKC1CR Test Number: 307 Daily Chemistry:
Day (Analyst identified for each d' '.-~---------~---~--------;~
nerfbrmed nl-LD.O, and conductivity measurements only.
0 2 Analyst !i) A/ iI~A3 ~& IISi~ I~u.) II ft/&
Concentration Parameter i
pH (S.U.) ~oo If-~qq-
- IIF *7.1l~rI-l~c~ H 70 q6
~sJL~I
]DO (mag/L) 7,'
Conductivity 30 32g.
CONTROL
- Hardness (mg CaCOsL) _____
~o.% ~¶
_ __ ___ ~
IV*. ............... '- . 6*.ij _. II o I ,1WI !I *
- pHo(S..) __,i ____ __ .___ , . 17 45gK IL Conductivity " ___"
______ *Temperature (%-C) "RA, * "1A[*. -*q*i~A "*,0 ' "'ll 400 mig KCIIL Conductivity "
0
.......... *Tremperature ( C) -- '- . = ",A~ ' "L, .1 "iO 600 mg KCI/L Conductivity Llf2O- *** l_
DO(m*L) . , 14*' 77".'
900 mg KCI/L Conductivity l O*" [ *O
........ __.. ..... _ *Temnperat~ur e(;oC). 1AO*, *1$ ll _S1.. ._.1A. . ...
pH(&.u.) ,71(*
DO(rag/L). 17,' -I '~~-
1050 mg KCIIL [Conductivity _, -- /-
~~AiO
- 'Temperature (00) a Finamfil Initial Initial
- *Temperatures performedperformed nity and hardness at the time byofthetest initiation, analyst renewal identified or termination on the bench sheetbyspecific the analyst identified for each analy~sisinand the Daily Renewal transcribed Information to this tableby:located@og Page 1.
bench sheet
Page 84 of9 SO A 2 -Ehii "TIleiin1-11
Page 6of6 Gdr.n~ntaJTeitIn;S.ri.t!o.s.!ne.
Species: Pimevhkales promelas PpKC1CR Test Number: 307_
(Anlvsidntile fo**Day (Anlys Jdntiiedforeach day. i~erformied pH, fl ity measurements only.)
6 AnalystF ' t*,
k cb*
Concentration Parameter pH (S.U.)
DO (mgIL)
Conductivity
(~axnhoskm)
CONTROL *Mkahinhiy (mg CaCO3/L)
- Ijt~rdn~
(mg CaCO 3/L)
- Temperature a.(0C) pH (s.u.) - *'2 I-DO(rng/L) 300 mg KCI/L Conductivity
(~+/-mhos/cni)
- Temperature (0 C) 450mg KCI/L Conductivity *0 *il' ' l *
- l7., r l DO(mg/) 7.7 h7,i z 600 mg KCI/L Conductivity 75mg(SL .nU.)vi *7 16I 3o 1/ . -
750 mg KCIIL Conductivity
- 2 0 * "g (uimhoscm) 9050 mg KCI/L Conductivit y*o **
DOTemperature initial Final Initial *inai Initial *nal Iaitial Final E.;ve ratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located tin Page 1.
A1¢l/ity Ai I and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: _.**
\ Y" / SOP AT2I - Exhibit AT21 .1,revision 11-*01 -14 Page 85 of 98
Ceriodaphniadubia Chronic Reference Toxicant Control Chart 4 Environmental Testing Solutions, Inc.
J I I I I 1.14 "USI* i I I I I J I I I I I I I I I
- PA Control Limits (IL2 StandardDeviations) 1-.12 1.10 1.08
- 1.06-boe~~o.........................*.....,..***t***~b**
1.04 ............ ................ ooem 1.02 1 I I I I I I I I I I I I I I I i I I 2.5 , I I I I ,. ,I I I Ii 1 i I I I I S 2. USEPA Wantning and Control Limits (75t and90t PercentileCl's)
,, 1.5 e l peo oo e el Q 1.0 . S S S 0.5 .. *f9*................
I
- I* , 1 I I I I I I I I I i I .1 I I l
. . . . . I 1 I 1 I 1 1.4 I I I I t i I i I I I I I I I I j j J I Laboratory*Warning and ControlLimits (J 0 th and 2 5 tA Percentile CVs) "
1.3 1.2 1.1 1.0 0.9 Air I I I I 1 I I I I I I i I I I i i i I I
- Test date
-.- 7-day lC 25 = 25% inhibition concentration; An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- . Central Tendency (mean IC2 s)
--- Warning Limits (mean IC25 + SA10 or SA.TS)
CnrlLmt(maIC5 + S 5 .0. 9O or Stndr Deiaions).
Gruphsg[erwrated fromeuroclnted excels*pr*,ads heet,
- Page 86 of 98 En.Ispr.dsh..t entered by:JSme Rmv~uwud y J u. .
Co Ceriodaphniadubia o* .. Chronic Reference Toxicant Control Chart CO Environmental Testing Solutions, Inc.
State and USKPA Laboratory USEPA USEPA 1-alsoratory Testnumiser Tesedate "7-dayiC *3 CT S Control Limits Se.so Warning Limits S* Control Limits S,.T Warning Limits S* Control Lmits CV (g/t NaCI) (8/INaeI) CT-2S CT+25 cT-s, a*L'T÷s,.w CT -S5un C-'C÷ u.n aT- SA,* aT ÷ SJLT T*a.;5 Cr-÷ S~J0 04-08-14 1.05 1.06 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.88 .1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 2.
05-05-14 1.05 "1.05 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.68 1.24 . 0.4.8 0.58 1.54 0.56 0.40 1.72 0.01 3 06-03-114 1.05 1.05 0.01 1.04 . 1.08 0,06 0.98 1.15 0.18 0.88 1.24 0.48 0.58. 1.84 0.86 0.40 1.72 0.03.
4 07-08-114 1.07 1.06 0.01 1.04 1.08 0,08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 ' 0.88 0.40 1.72 0.01 S 08-05-14 1.06 1.05 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.88 1.24 0.46 0.58 1.54 0.65 0.40 1.72 0.01..
6 09-09-14 1.07 1.08 0.01 1.04 1.08 0.08 0.98 1.15 0.18 " 0.88 114 0.48 0.58 1.54 0.66 0.40 1.72 0.01 7 10-07-14 1.08 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.16 0.88 1.24 0A48 0.58 1.54 0.66 0.40 1.72 0.0+/-
8 10-07-14 1.00 1.06 0.01. 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1124 0.48 0.58 1.54 0.66 0.40 1.7"2 0.0"1 9 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.58 0.40 1.72 0.01 10 12-02-14 1.08 1.06 0.01. 1.04* 1.08 0.09 "0.98 2.15 0.18 0.88 1.24 " 0.48 0.58 1.54 0.65 0.40 1.72 0.01 11 01-23-15 ,1.06 1.06 0.01 1.04 . 1.08 .0.09 0.98 1.1.5 0.18 0.8"8 1.24 0.48 0.58 1.54 " 0.68 0.40 1.72 0.01 12 02-03-1.5. 1.07 1.06 0.01 1.04 1.08 0.09 0.98 :1.5 .0.1.8 0.88 1.24 0.A8 0,59 1.54 0.55 GAO0 1.72 0,01 13 03-01-15 1.06 1.06 . 0.01 1.04 1.08 0.09 0.99 1.15 0.18 0.88 1.24 0.46 0.59 1.54 0.68 0.40 1.72 0.01 14 04-07-15 1.06 1.05i 0.01 1.04 1.08 0.09 0.98 1.15 0.16 0.88 1.24 0.48 0.80 1.54 0.88 0.40 1.72 0.01 15 05-05-15 1.07 1.05 0.01 1.05 1.08 0.09 0.98 1.135 0.18 0.88 .125 OAK8 0.59 1.54 0.65 0.40 1.72 0.01 16 06-09-15 1.08 1.06 0.01 1.04 1.08 0.09 0.90 1.15 0.10 0.88 1.25 0.48 0.59 1:.54 0.66 0.40 1.72 0.01 17 07-14-15 1.06 1.07 0.o1 2.05 1.08 0.08 0.98 '115 .0.19 "0.88 1.2.5 0O48 0.59 1.54 0.66 0.40 1.73 .0.01 18 08-04-15 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.4B 0.58 1.55 0.58 0.40 1.73 0.01 19 09-15-15 1.07 1.07 0,01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.56 0.41 1.73 0.01 20 10-06-15 1.08 1.07 0.01 1.05 1.08 0.09 0.08 1.1`5 0.18 0.88 1.25 0.45 0.59 1.54 0.55 0.40 1.73 0.01 Note: 7-d ic* = 7-day 255%inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Cerionlophnio reproduction for the test population.
CT Central tendency (mean 1Cm)..
S =Standard deviation of the lC.vs vlues.
Lab oratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the iC* values corresponding to the 10th and 25th percentile Ct/n. 'These ranges are more strngent than the control and warning limits rtcom~nended by USEPA for the test method and endpoint.
-* Standard deviation correspondingto the i10' percentile CV. (5z 0.0B)
Sz = Standard deviation corresponding to the 25e" percentile CV'.(S~z = 0.17)
UJSKPA Conical and wamning Limits S**= Standard deviation corresponding to the 75" percentile CV. (Sa = 0.45)
$un = Standard deviation corresponding to the9Oe percentile CV. (5S~ = 0.52)
CV = Coeffitient of variation of ithelIwvalues.
USEPA. 2000. Understanding and Accounting for Method Variabli1ty In Whole Effluent Toxicity Applications Undor the National Pollutant Discharge Elimination Program. EPA-833-lt-00-003. US Environmental Protection Agency, Cincinnati, Cl-.
FRe: CdNa~CIt_100l5.nisx Entered by: J. Sumner Reviewed hy.
4"Enromnl I Ceriodaphniadubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 - I I I I I "I I II I I I I I I I I
- I I -
35 0
30 0
25 I-0 20 0
0 Q 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
- I I I I 1 I I I 1 I I I 1 1 I I -
I I I I i I I I I. I I I I I .I I I I I I I S40* North Carolina Acceptance Limit (< 40.0%)]
Kentucky Acceptance Limit (<30.0%)
c* 20 50 E
- II I I I I I I I I I I I I I I 1I " I 40 K USEPA Upper PMSD Bound (90t percentile < 47.0%)*
0 30 20 10 I I.I.."....I..I I I I I'* I* I I I .I. I "
"I
- 4~W 8
~A ' ~9' ~~.Xah~1AN XIrX 5 4~V~ ~ ~ ~ ~ ~6-~
Test date
--- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
-- -. Central Tendency (mean Control Reproduction, CV, or PMSD)
.** Control Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)
Gi~phs generated flora augcl.ted eac.I.pre.dshe.t.
- Page 88 of 98 "" toed ,preadsheet enteredby: J. Sumner Renlawedby:
Precision of Endpoint Measurements Ceriodaphniadubia Chronic Reference Toxicant Data SEnvironmental Testing Solutions, Inc.
Testnubr Test date Control SControl Mean Survival Reproduction. Cr CV a~ MSD PMSD a1 for Control Mean for Control Reproduction (%5) .Reproduction (95) PMSD (%)]
(95) {offsprlngffemale) .for (offspring/female) CV (95) 1 04.08-14 32.4 6.7 2.1 6.5 2 05-06-14 1O0 31.3 31.9 5.2 6.0 .1.7 5.5 6.0 3 06-03-14 100 34.6 32.8 3.9 5.3 2.3 6.6 6.2 4 07-08-14 1 00 34.5 33.2 3.1. 4.7 1.7 4.9 5.9 5 08-05-14 100 34.0 33.4 5.0 4.8 2.3 6.8 6.1.
6 09-09-14 100 32.6 .33,2 4.6 4.8 2.0 6.1 6.1 10-07-14 100 29.7 32.7 4,8 4.8 2.2 7.4 6.3
- 8. 10-07-14 100 32.1 32.7 3.1 4.6 2.1 '6.6 6.3 9 11-04-14 100 29.8 32.3 5.0 4.6 1.8 6.2 6.3 10 12-02-14 100 33.1 *32.4 6.4 4.8* 2.0 6.2 6.3 11* 01-13-15 100 32.7 32.4 3.8 4.7 1.8 5.6 6.2 12 02-03-15 100 32.5 32.4 3.9 4.6 1.8 5.6 6.2 13 03-03-15 100 32A 32.4 6.0 4.7 1.9 5.8 6.1 14 04-07-15 100 32.3 32.4 4.6 4.7 1.8 5.5 6.1 15 05-05-15 100 31.7 32.4 5;.6 4.8 1.8 5.8 6.1 16 06-09-15 100 29.7 32.2 2.8 4.7 1.4 4.8 6.0' 17 07-14-15 100 30.8 32.1 3.0 46,6 1.5 4.8 5.9 18 08-04-15 100 31.0 32.1 3.0 .4.5 1.6 5,0* 5.9 19 09-1.5-15 1O00 29.4 31.9 4.0 4.5 1.7 5.9 5.9 20 10-06-15 100 29.6 31.8 4.8 4.5 S 1.8 6.1 5.9 Note: CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (l10lpercentlle) = 8.9%.
Upper CV bound determined by USEPA ( 50 thpercentile)= 42%
MSD = Minimum Significant Difference PMSV = Percent Minimum Significant Difference PMSD is a measure of test preci~.ion. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a *vihoie effluent toxicity test.
Lower PMSD bound determined by USEPA (2 0 t percentile) = 13%.
Upper PMSD bound determined by USEPA (90t percentile) = 47%.
aT = Central Tendancy (Mean Control Reproduction, CV, or PMSD).
USEPA. 2000. Understanding and Accounting for Method Variability in Whale Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPAo833-R-0D-003. US Environmental Protection Agency, Clncinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-D-01-004 and EPA-821-B-01-005. US Environmental Protect~on Agency, Cincinnati, OH.
"*' .... File: CdNaCICR._1006]5.xlsx Entered by:, J, Sumner Page 89 of 98 R*ewby;__
Page 1 of 6
.1 E~oI~do.od~.
Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodayhnia dubia CdNaCICR #: 17..99 Dilution preparation info'rmation: ,.... Comments:
NaCI Stock INSS number: INSS Iflbl __-___.........._______
.Stock preparation: 100 g NaCI1L:"
Dissolve 50 g NaC1 in500 nmL Milli-Q water.__________________
..D~iuton prep m*)'1 600 800 1000 1200 1400 .......
St1 oue(L 2 .I 15 . 18, , 21 _ _ _ _ _ _ _ _ _ _ _ _ _
DilentvolumeQ(m) 491 1488 1485 1482 1479 ,- . ._______________
Ttloue(L ... 50, 1500 1500(J1500 1500 _________ _________
Test organism source in ormaaion: Test information:.
Or anism ae: < 24-hours old Randomizing* template color:
Date and times organisms were born Gto~-Ce 0 't b tL. Incubator number and shrif between: . . .. location: 2*
Culture board: -* t**
Replicatenumber: I 2 [3 [4 ]S ]6 [7 8 9 10 YWTbatch:
Cultureboardeupnurnber: Ia I ]iiq It I Z*ItIk[lo~*0"'
0 Transfer vessel information: H -- ,o. _SM.. Ternperture = -- *,*C Selenastrum batch:
- Average transfer volume (mL): 0.012 1 joL .. 0*q° Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used
-_ termination time
.0O1-06-15 . ___ "_____._.____ o:',.,,
1* 1 10-07-15 .______________. (*t 1 eot "*
2 10-08-15 _____________ *o'.0V¢s
__ __ 10.09-1S ..
________________ t-to- a
____ 0-11-15 e3._o*O *,"O-r h )
6 10-12-15 SControlinformation: _ ___ Acceptance criteria -Summary of test endpoints:
% of Male Adults:
%Adults having 3 rd Broods:
%Mortality:
. 0~1 ,<20%
O1. ,.<20%
. 80%
7-day LCs0 NOEC LOEC J "7 1
.,t2 cO _
I*
I Mean Offspring/Female: 2A.,* h 15.0 offspring/female ChVY j t1( .S Indepndet
~Anw by age 90 of 98 SOP AT14 - Exhibit ATI4.1, revision 1 1-01-14
D 0
Page 2 of 6 a
-,J Species: Ceriodaphaniadubia CdNaCICR #:. 17_.9 CONTROL Survival and ReproductionData
_ __ _* - Replicate number . __
Day___ 1 2 3 4 5 [6 7 8 .9110
[Adult mortality k. .J_ . 1- ~
- I...
2f] Young poduced *, I
..... >... .CL..O .C1. 0 I I I__
(J(J *:1 I Youngprod,,ced Adult mortality f_ [ ___ f1 Qi j
!.I... [...
Q) 4 I YoungprodUCed 1W1I.. ~I A..~I~I-
____ I Young produced ~~LV'~ O %L i 6[Adult mortality \. ___Y___ngproduced__
Total young produced " *I -.. I 7.* -. I7,"?. ,* I 1.. I . V'* 3o Final AduitMortalit \.- I W I .
's -. 4.. " t:2 -- :-* t.. c-- '...
Note: Adult mortality (L =f live. D = deadil. 51B= s ro ml nlit ro ot between two daysi. CO = carry over (offhorinc carried over with adult during transfer). ...
II Ill I I Concentration: I.
~%Mortality; I (37 I
-Replicate niumber Day 21 $ 6 7 8 9 10-C:
1 Young produced Adult mortality 010
- . *.J 0..1 010O c~ioic~ic 21 Young producedIl Adult mortality cflL.- ]* k.[I .. J*
I L_[j* *L J I....] I (3-1 L,.j <J L.,- I L-- c -,..
I" 3 _Youngproduced i *1JO CL I 0O A1 fl cl I J
.~I Adult mortality *' JL L.* 1'*--I 1 *" I*-1 "-- I_ I" I-"'
4 Youngproduced '-. " k.I*_ .. *** .. [*_. * [. ,
SAdult mortality J '...\..__ .- J
'."J_ L"~ ' - L.
[.Young produeed I. Lt. k** [,I If _____ ] ____ _ ___lL
'Adult mortality ~ IC"
- j- L. ' I *.. [,,- ':~
Adl ...m ralt * . ._U.,,__ -- [ . [ *.
Young produced. 0$ II i* l .1C 0 0 IA * '*
Total young produced "* * "I_'* *- "*-Z *L *( *(o *'% -
Final Adult Mortality * *. ... *...
i**. -.. cr
- caridedAdult
- Note: mortality over with (L =live, adult during D = dead), SB = split brood (single brood spilit between .two days), CO =carry over (oftspring transfer).
I%Concen~tration:
Mortality:
Mean Offspring/Female: I 0.
¢07 IadQ.~d.nt
% Reduction from Control: - ***
Misy E. ~
SOP AT14 - Exhbit ATI4.1, revision 11-01-1 4 LA..-
Page 91 of 98
Page 3 of 6 Species: Cerlodaplhnia dubia CdNaCICR #: 17.99 80 ~NaCIIL Survival and Reproduction Data
__a _ __ 1112 T ____ _,,,
3 4 Replicate number 5 6 7 8 .9 10 2 Young produced- C) T2 (t 01 D 0 o.O 1 Adultimortality "- 1 Z . '- - '-I . -I'-
2 [. Young produced-1.c. __
Adultgportality Jl 'C I '-I C- I '- I s-'aI_'0 I50 ___
Adult mortlitY j k_ *--- U:- .,-.J_U U * -A 1 '-1'- __-
61I Young produced JCj I i I )I I--1 0 ) 0
-I Note- Adult mortality (L = live, D =*dead), SBS - split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). ____________________
IConcentration:
0 AMortlity: 17.
SMean Offspring/'Femnale: I 3. t*
I%Reduction from Survival andReproduction Data Control:" "-5.'1 ,I, 1000 m~NaCIIL
[ 1
____________ 5 number 6Replicate 8 9 1 Day__ 2_ 10617 8 9 1 Young produced * £ _0 0*
- 2 ~Young produced ] 9 0 t "-0 Adult mortality ~ ~ _ __ _ _
4 Young prodUced __ _ __ _...o __
S mortlity YunpducAdult ___ __ __ j, L I.
- .[,
4oa young produced 1 1 II2k 1U~ !~j ialAdult Mortality j ..,T pL,_, , _ L * .q__._ ___
ote: ( lieJ-Aduit mortality edS ' -slibod(ine broo splt bewe[ \4_ tw. das) 'O -I--Icryoe (ofp carried over with adult during transfer).
Concentration.
h.do~.,d7nt Mean Offspring/Female:
- 1_2 R..i.,, hy Reduction fro.m Control: I Y.II.yLX.rnfl
-°4 7. S%
SOP AT14 - Exhibit ATI4. 1, revision 11-01-14 Page 92 of 98
Page 4.of 6 Species: Ceriodaphniadubki CdNaCICR #: 1 7_99 i1200 m ~NaCIl* Survival and Reproduction Data
........... Replicate number Day ______ 1i 2,3 14*___ I5 6 718 9
1 I!~Young produced Adult mortality L. *...__'
01~
I" .. '-_ .. '-
O~
I.__-I *..s-'
II Young produced 01 10 : . OL i0
- 4- ".
- i
__ Adult mortality _ ~P '
Adult mortality '-__If-____* -.
Young produced O~_~~ * " IiILA -OL J Adultmortality*[
- lI 6 I 6[YoungAdlmrat produced dl otliy Yon prxod.,uced j
I 4
f* ]
5 1."'4 I.-
L...o...
- ).
L. .j--
0
.ŽL I
£
....o..'.
el Total young produced [* * * .* *
% l*
"*g ]* t t%
Final Adult Mortallt ' ..- %-.- %. %-- %.: '-- %.--' -
Note: Adult mortality (1.= live, D = dead), SB =split brood (single brood split between two days), CO = carry over (offspring carried over with adult daring transfer)._____________________
IConcentration:
-%oMortali: 1O' IMean Offspring/Female: I
- I
% Reduction from Control: IA.tu 1400 mg NaCIIL Survival andReproduction Data
_______ ___Replicate number ____.
Day ________, 1 2 1 3 1 7, 8 9 110
___j .Adult moilalit L_. * .. L.*
=. _ I _ 1 - I-2I Adult mortalityj j.~~ - L ~ t~-I. L.
.6 IYoung produced Adult mortaliy ",*
_ _ j)
°'_ _ _
I Young producedJ(* (.* I* . t0 C)I J " L ( I:)
T ~otalong prodluced
- 0 L C) 101 : ")" C) ,0 O *.'JL
_Fna Adult Mortality %.. ______ __I'.. _'-I'.- I_'
irene: ImorflUIIy tt. = liVe, J.I =ogaU), *fl = IpilI e~rUoUIS1ngIO OIoUU *pZI oOIWCCJn IWOueys), LU = mrly over moyu~pnim carried P.UUlt over with adult during transfer).__________________
S IConcentration:
flMean Offspring/Femal'e: I2~0
% Reduction from Control: **'T' SOP AT14 - Exhibit ATi4:I, revision 11-01-14 93 of 98
Verification of Ceriodaphnia Reproduction Totals 0
- Environmental Testing Solutions, Inc.
CO co Control 1000 mg NaCIiL-
- Replicate numberToa Day 1 2 3 "4 Rep)icate numberToa 5 6 7 8 9 10 Tta Day ~1 2 3 4 5 6 '7 8 9 10 Tta 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 00 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0* 0~ 0 0 3 0 0 0 0 0 0 0o 0 0 0: 0 4 -" 4 3 4 3 3 3 4 3 5 3 35 4 4 5 3 4 5 4 5 3 3 3. 39
- 5 10 12 12 9 10 12 12 11 9 1I 108 5 12 10 11 11 9 10 10 10 11 10 104 6 0 .0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0
.7 15-13 13 16 16 15 16 18 151I6 153 7 15 18 15 13 15 12 15 14 14 15 146 Toa 29 2 29j28 29 30 32 32 29 30 296 Total 31 33 29 28 29 26 30 27. 28 ,28 289 600 mgNaCFlL 1200 mgNaCI/L_______
liReplcate numberToa Day Relct number ,- - Total Day 1j 2~ 3 4 5." 6 7 8 9'
- I Tta '1~-* 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0. 0 0 0 2 0 a 0 0 0 0 0 a0 0 _ 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0.0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4. 3 3 4 4 4 4 4 3 4 37 4, 4 2 28 5 12 10 12 11 13 12 101I012 10 112 5 0 0 3 0 0 54 0 0 3 15 6 0 0 0 0 0 0 0 0 0 0 0 *6 7 8 .0 5 40 0 74 0 35 7 15 17 14 17 15 16 16 16 18 14 158 7 0 0 4 0 0 3 5 0 0 0 12
- - - - - - a - - - -i a..........7 Total 31 30 29 32 32 32 30 30 33 28 307 Total 9 11 11 7 7 11 13 8 8 5 90 800 mgNaCIIL 1400 mg NaCI/L Da Re *licate numberToa Da - Re lieate nuberTotal Dy 1 2 3 4 5 *6 7 8 9 10 Tta Dy 1 2 3 4 5 6 7 8 9 10
- 0 0 0 -0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 .1 0 0 0 0 0 0 0 0 0 0 0 2 0.0 0 0 0 0 0 0 0 0 0 2 0 a 0 0 0 0 0 0 0 0 0 3 0 0 _0 0 0 0 0 0 0 0 0 4 4 5 4 4 3 4 4 4 4 4 40 4 2 0 1 3 2 1 1 2 1 2 15 5 11 -12 12 10 11 11 11 13 12 12 115 5 0 2 0 0 0 0 0 0. 0 0 2 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 17 13 1.5 15 16 13 16 13 151 7 0 0 1 0 0 0 2 0 0 0 3
-I- - II- - - . ** *. *-
- a *. . - . I Total 32 33 33 27 29 30 31 30 32 29 306 Total 2 2 2 3 2 1- 3 2 1 2 .20 File: CdNaCICR_10061 5.xlsx Entered by: J. Sumner Reviewed by:
to O'*P
- r]f*"Ceriodaphnia
- i:EPA-821-R-02-013, dabia Chronic Reference Toxicant.Test Method 1002.0
- EwlrnmetalTesingSolutions, Inc. Yeiiaion of Dat Entry, Calculations, and Sta~tistica Analyses Test number: CdNaCICR #179 Test dates: October 06-13, 2015 Concentration _____Replicatre number _ __ ___ Survival Average reprodnction Coefficien of Percent rmlaetii from (rng/L NaCG) 1 2 3 4 5 6 7 8 9 10 (%) (offspringlfemale) VSriTIZIO (55) cootrol (°"A)
Control 29 28 29 28 29 30 32 32 29 30 100 29.6 4.8 Not applicable 600 31 30 29 32 32 32 30 30 33 28 100 30.7 5.1 -3.7 800 32 33 33 27 29 30 *31 30 32 29 100 30.6 6.4 -3.4 1000 31 33 29 28 29 26 30 27 28 28 100 28.9 7.0 2.4 1200 9 ii 11 7 7 11 13 8 8 5 100 9.0 27.2 69.6 1400" 2 2 2 3 2 1 3 2 1 2 100 2.0 33.3 93.2 Bennett's MSJD value: 1.813- MSD ='Minimum Significant Difference PMSD: 6.1 PMSD = Percent Minimum Significant Difierence PMSD is a measure oftest precision. The PMSD is the minimum percent difference between'the control and treatment that can be declared statistically significant Ena whole effluent toxicity test Lower PMSD bound determined by USEPA (10"' percentile) = 13%.
Upper PNISD bound determined by USEPA (90"' percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th petcen/tile, respectively, of PMSD data from EPA's WET Interiaboratory Variability Study (USEPA, 2001 a; USEPA, 200 ib).
USEPA. 2001a,.2001b. Final Report: lnterlaboratory Variabiliy Study of EPA Short-term.Cbronic and Acute Whale Effluent Toxicity Test Methods, Volumes I and 2-Appendix. 2PA-421-B-01-004 and EPA-821-B-01'-00S.
US Environmental Protection Agenty, Cincinnati, OH.
File: CdNaCICR_100615.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J.-Sumner Reviewed by':
0 a
a Statistical Analyses 2
~ E1vIroflm~nt5Te,¶IngSoIutIons, inc.
Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date: 101612015. Test ID: OdNaCIOR ,Sample ID: REF-Ref Toxicant End Date: 1011312015 Lab ID: ETS-Envir. Testing SoL. Sample-Type: NACL-Sodlum chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Teat Species:. CD-Ceriodaphnia dubla Comments:.*
Cone-mgIlL 1 2 3 4 .5 6 7 8 9 10 D-Control 29.000 28.000 29.000 28.000 29.000 30.000 32.000 32.000 29.000 30.000 600 31 .000 30.000 29.000 32.000 32.000 32.000 30.000 30.000 33.000 28.000 800 32.000 33.000 33.000 27.000 29.000 30.000 31.000 30.000 32.000 29.000 1000 31.000 33.000 29.000 28.000 29.000 26.000 30.000 27.000 28.000 28.000 1200 9.000 11.000 11.000 7.000 7.000 11.000 13.000 8.000 8.000 5.000 1400 2.000 2.000 2.000 3.000 2.000 1.000 3.000 2.000 1.000 2.000 Transform: Untransformed 1-Tailed Isotonic Conc-mgI/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 29.600 1.0000 29.600 28.000 32.000 4.831 10 30.300 1.0000 600 30.700 1.0372 30.700 28.000 33.000 5.104 10 -1.387 2.287 1.813 30.300 I )0000 800 30.600. 1.0338 30.600 27.000 33.000 6.389 10 -1.261 2.287 1.813 30.300 1.0000 1000 28.900 0.9764 28.900 26.000 33.000 7.006 10 0.883 2.287 1.813 28.900 0.9538 "1200 9.000 0.3041 9.000 5.000 13.000 27.217 10 25.976 2.287 1.813 9.000 0.2970 "1400 2000 0.0676 2.000 1.000 3.000 33.333 10 34.803 2.287 1.813 2.000 0.0660 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p
- 0.01) 0.82283 1.035 0.1504 0.06135 Bartlett's Teat indicates eq~ual variances (p = 0.02) 13.1798 15.0863 Hypothesis Test (1-tail. 0,5)1 N4OEC LOEC ChV TUJ MSflu MSfl MSB MCE F-Probh df Dunriett'sTest 1000 1200 1095.45 1.81338 0.06126 1647.58 3.14444 2.0E-44 5,54 Treatments vs D-Control Linear interpolation (200 Resamples)
Point malL SD 95% CL Skew IC05 1001.16 37.4909 906,468 1012 -2.0108 IC10 1018.38 6.35554 1003.22 1027.02 -0.1934 1C15 1031.61 5.9215 1018.58 1042 -0.1085 1.0 1Q20 1046.83 5.58724 1035.25 1057.01 -0.0406 0.9 8
1025 1052.06 5.34593 1051.32 1072.01 0.0262 1C40 1107.74 5.28361 1097.31 1117.31 0.1552 0.8 IQ50 1138.19 5.78951 1127.31 1148.64 0.2091 0.7 0.6 S03 S0.3 0.2 0.1, 0.0 ... - . . .,
-0.1 0 500 1000 1800 Dose mg/L Dose-Response Plot 30 1-tail, 0.05 level of significance 25 208
- .15 10 e , , . .
I5 0 o
o 0 r nd.eod.nt C.
b~4.wby e File: CdNaCICR_1006 ] 5.idec Eatered by: .1.Sumner Reviewed by:
Page 96 of 98
0 Page 5 of 6
~;yi~i ~.
~
CdNaClCR #: 17..9 Species: Ceriodavhniadubia Daily Chemistry: ____________________________________
I m
Day (Analyst identified for each day, performed pH, D.O. and conductivity' measurements only.) I i -
'I I 1 I Concentration Parameter
- I--
pH (S.U.)
DO (rag/L)
Conductivity (u~mhes/cm)
CONTROL *Allnslinity (mg CaCQ 3 IL)
- Hardne5s (inn CaCO~/L)
- Temperature B - *~ih. - 6 pH (s.u.) II 7A~ I 600 mg NaCI/L DOQng/L)
Conductivity IZAI~1LII 7, ~q. I (puhos/em) 1q70 ~37O I.
'*Temperature (°C)
U -
A- Ip pH (s.u.) '4~q3 7~6 1.
L 800 mg NaCI/L DO(mglL)
Conductivity
(~ernhosIcm)
II rtLIo I U
7 jgt~ v15v 1-Ih TCflhJJCflItUT~ ('C) l.A L.qt ,.Y pH (s.U.)
B 1h 224 ,7.q*
1000 mg NaCIIL.
i I conductivity DO~mg/L~
I*Temperature ('C) pH (S.U.)
B 2O~iO 1-'v*. ~II -
1.7 IA,. h --
8.0 2.q.,*.-
VIO 1~6
- qp 7.7 DO (mg/L) e.0 'R g 1200 mg NaCI/L Conductivity
(~+/-mhos/cm)
~43o
- Temnperatulre ('C) 15V1 qp~ 6 pH (S.U.) 7.e7 7,,Rq '1.
DO (mag/L) 1400 mg NaCI/L Cond:etivity F*Temnperature
.. ('C)
I ~'o Initial21L IIJ.*
II FinallIl- nitiallIl J. &I MA' Final
~28tO 24.9
-rInitial
" 11 7"'" =
Final
- Tempeiaturetable lnformation performed located on at the Pagetime of test initiation,
- 1. Alkalinity renewal'performed and hardness or termination by*thebyanalyst the analyst identified identified on theinbench the Daily sheetRenewal specific for each analysis and transcribed to this bench sheet by: ....
- _
Page 97 of 98 SOP AT14 - Exhibit AT14.1, revision 11-01-14
0 Page 6 of 6
¶~ EtVCfiffi.atilTOg5OhALfl~I~~
Species: erodaphniadubia CdNaCICR #: 17_9 Day (Analyst identified for each day, performed pH, D.O. and conductivit measurements only.
3 4 5 6 pflO(S.,.) "7,*1 - "*. " o-
- 'Conductivity 30738 CONTROL *Aflkainty ~2 600 NaC mg (mgCaondctviy
[, .
- j .pH(S.U.) 7.# . 5 1" . i7" q,* Lq -____ -q .
Do (mg,) 7.__7__l,.3 l /i 600mng NaCJIL °*(*-Conducteit iS3o -0 1*7 y
8400 mg NaCI/L Caldc*vt (ishscm)
Initial Final Initial ifFinal jInitial I[Final IInitial IFinal
- Temperatures Information tableperformed at the located on Pagetime of test initiation,
- 1. Alkalinity renew'alperformed and hardness or termination by thebyanalyst the analyst identified in the Daily Renewal.
identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: L_ .. .
SOP ATI4 -*Exhibit ATI4.1, revision 11-01-14 f*
Page .98 ofr 98
Attachment 4 Corrected DMR Page 101T
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR) MAJOR (SR01OMNo24-04 Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
.(.INTEROFFICE OPS-EN-SQN)
TN0026450 [101 T ]F- FINAL
___ SODDY_ .- D._AISY.,IN.37384_ PERMIT NUMBER [DISCHARGE NUMBERJ BIOMONITORING FOR OUTFALL 101 Fgaciliy TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MO~LRING PERIOD IEFFLUENT Froml 15 10 01 fTot 15110131 ] - OOAO ATTN:Millicent Garland NOTE: Read instructions before compIeting this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_______ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 1C25 STATRE 7DAY CHR SAMPLE ********** >1 00.0 **** ****0 1/10CMO CE*RIODAPHNIA MEASUREMENT 23 0 110 CMO TRP3B 1 0 PERMIT ********* **42.8 ********* PERCENT SEMI COMPOS EF FLUENT GROSS RUIENTMINIMUM ANNUAL 1C,2;5 STATRE 7DAY CHR SAMPLE ********* *>100.0 ******** 3 0 1/10CMO PIMEPHALES MEASUREMENT23 0 /10 CM S TRP6C 1 0 PERMIT **** **** ** 42.8 ************* PERCENT SEMI COMPOS EF FLUENT GROSS REQUIREMENT MIMINUM ANNUAL____
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL ExECUTIVE OFFICER I Certify under penaltyof law that this document and allattachments were prepared under my AEELEPHONE Te 15DATE 1
______________________________________ - irection or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the lperson or persons who manage the system, or those persons directly responsible for gathering <-S1ViePsiet42 83701 5 12 0 SieVc Peietthe information, the information submitted is, to the best of my knowledge and belief, true, 423_______843-7001____________
Sit reidet Vce accurate, and complete. I almaware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE -
information, including the possibility of fine end imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT ARA NUMBER YEAR MO DA-Y-TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled October 18 - 23, 2015.
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 December 10, 2015 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR November 2015 Enclosed is the November 2015 Discharge Monitoring Report for Sequoyah Nuclear Plant. Per your request, Attachment 1 is also enclosed which summarizes the average monthly flow from Outfall1101 for June - October, 2015. The Turbine Building Sump was aligned to the Yard Pond November 20 -
27, 2015. Turbine Building Sump monitoring data is summarized in Attachment 2. The final report for Toxicity Testing conducted October 18 - 23, 2015 is also enclosed as Attachment 3. Attachment 4 shows the corrected October DMR page for l01T (based on receipt of the final Toxicity report).
There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.
I certify underpenalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gatherand evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, Sequoyah Nuclear Plant Enclosu res cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk Chtaog,4State caleOffice Building,AeuTnese Suite370_0550 3Washington, DC 20555
- PERMrITEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
Address P.O. BOX 2000
.._(.IJNTEROFFICE OPS-5N-SQN)
S TN0026450 I[101 G F -FINAL.
SODDY - DAISY, TN 37384 S PERMIT NUMBER I[DISCHARGE N-UM-BER] DIFFUSER DISCHARGE Facili_ TVA - SEQUOYAH NUCLEAR PLANT EFFLUENT Location HAMILTON COUNTY I MONITORING PERIOD I MO I DAy NO DISCHARGE [*
ATT-N:Millicent Garland From!LYEARI 15 11 01 ILYEARI Tol I1 MO 11 I130 DAY NOTE: Read instructions before completinpa this form.
PARAMETER QUANTITY OR LOADING "QUALITY OR CONCENTRATION NO. FREQUENCY! SAMPLE
_________-EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ********** "********** 32.7 04 0 30)! 30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 PERMIT ***** **** ** **** **** Req. MOn. DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE **** ****** ********** 20.9 04 30 / 30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT ***** **** .. ******** 30.5 DEG. C. CONTI CALOTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE **** ****** ********** 2.4 04 0 30 / 30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 1 PERMIT ********* **. *********5.0 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX ___ _NUOUS TRAMNFLOW,IN CONDUITpLNOR THRU MESRMNSAMPLE ***.. J
- 03 ......
- a.....30 /30 R*CORDR 50050 1 0 PERMIT ***** Req. Mon. MGD ****************CONTI RCORDR EFFLUENT GROSS RQIENTDLYMXNUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1360 ***** 03 03 0 30 / 30 CALCTD TREATMENT PLANT MEASUREMENT 50050 1 0 "PERMIT Req. Mon. ***** MGD ********* **** MGD CONTI CALCTD EFFLUENT GROSS VALUE REURMN OAGNUOUS CHLORINE, TOTAL RESIDUAL SAMPLE ***** **** * ***** 0.021 0.029 19 a 19 /30 GRAB MEASUREMENT 50060 1 0 PERMIT ***** **** ** **'**0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE *****0.2 62** 0 30 / 30 CALCTD CHANGE MEASUREMENT 6 82234 1 0 PERMIT *****2.0 DEG *************** CONTI CALCTO EFLEN ROSREQUIREMENT DAILY MX C/HRNUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thes document and all attachments were prepared under my i* -TELEPHONE DATE tirection or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the Site Vice President person or persons who manage the system, or those persons directly responsible for gathering the information, the intormation submitted is , tothe best of my knowledge and belief, true, i Vc P es n-42 83701 15 423____843-7001_______15__12___10_ 2 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED ___________________________I________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injection occurred: Flogard MS6236 (max conc. Was 0.05 mg/L, limit - 0.20 mg/mL),
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fot PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) rm Approved.
DISCHARGE MONITORING REPORT (DMRJ SB 1 I Name "FA- SEQUOYAH NUCLEAR PLANT 18No. 2040-0004 Address P.O. BOX 2000
- -.- INTEROFFICE OPS-hN-SQN)
TN0026450 101 T F-FINAL SODDY - DAISY TN 37384 ._ PERMIT NUMBER - DISCHARGE NUMBER] BIOMONITORING FOR OUTFALL 101 Fac*jj_ TVA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY !MONITORING PER1OD EFFLUENT ATTN:Millicent Garland From 15 I YEARI MO 11 I DAY 01 I Tol YEAR I MO 15 11130 DAY **NO DISCHARGE Z*
- NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUCENY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 1C25 STATRE 7DAY CHR SAMPLE ***** ****
- Monitoring **** 23***
CERIODAPHNIA MEASUREMENT Not- Required
-° 23_____ _
TRP3B 1 0 PERMIT ********* **42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS RQIENTMINIMUM ANNUAL 1C25 STATRE 7DAY CHR SAMPLE ******* Monitoring ********
PIMEPHALES MEASUREMENT Not' Required
-" 23_____
TRP6C 1 0 PERMIT ***** **** .. 42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS RUIENTMIMINUM _____ANNUAL _ _
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT
-REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER -ICertify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE
-direction or supervision in accordance withea system designed to asaure that qualifiedt._/"2 ,-\f*
Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the L.
person or persons who manage the system, or those persons directly responsible for gathering _ -_'"_ e Vice P r____.__. _____ 423 843-7001 15 12 10 the information, the information submitted is, to the best of my knowledge and belief, true, ________________________
Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al/attachments here)
Toxicity was not sampled in November 2015.
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Formi Approved, Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SB01OM No. 2040-0004 Address P.O. BOX 2000
---. (INTEROFFICE OPS-5N-SQN),
[ TN0026450 ][103 G [F-FINAL SODDY - DAIS.Y, TN... 373._84__. . . . S PERMIT NUMBER DISCHARGE NU0MBER] LOW VOL. WASTE TREATMENT POND F._aciji_/ TV._A. - S..EQ._.UOYAH N UCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATl-N:Millicent Garland From LYEAR15I MO 11 010 m° DAYI 15 II11 To LYEAR MO I1301 DAY *
- NOSHREI ODSCAG I
- NOTE: Read instructions before completin~q thils form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_________ _______________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ***** **** *7.0 *****8.7 12 0 5 /30 GRAB MEASUREMENT 00400 1 0 PERMIT **** ***** ** 6.0 *****9.0 SU ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE ......... ** *...** 15.8 15.8 19 0 1 / 30 GRAB MEASUREMENT 00530 1 0 'PERMIT **** ***** ******30,0 100.0 MG/L ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MIX MONTH OIL AND GREASE SAMPLE **** ***** ** <5.0 <5.0 19 0 1 /30 GRAB MEASUREMENT 00556 1 0 PERMIT ***** **** * ***** 15.0 20.0 MGIL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH____
FLOW, IN CONDUIT OR THRU SAMPLE 0.972 1.151 03 0 5 /30 INSTAN TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD .***************ONCE! INSTAN EFFLENT ROSSREQUIREMENT EFLETGOSMO AVG DAILY MX WEEK SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEJTrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thia document and all attachmenta were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified / ' *..
Ch ristopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the VcePeL p)erson or persons who manage the system, or those persons directly responsible for gathering -,---Slte Vc r e 2 4-01 15 1 Site Vice President the information, the information submitted is, to the best of my knowledge and belief, true, 423____843-7001_______15__12___10_
accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA{ NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) 11/20/15 - 11/27/2015.
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
AddreSs P.O. BOX 2000
- -- INTEROFFICE OPS-5N-SQN).
[ TN0026450 J 110 G F-FINAL SODDY - DAIS..Y, TN..373.84__. . . . I PERMIT NUMBER DISCHARGE NUMBERI RECYCLED COOLING WATER F._acJ* TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY _yMONITORING PERIOD EFFLUENT YEARl MO DAY YEARI MO DAY "'N ICAG FromJ 15 11 I01 To-l15 11- 30
- OOARE @
- ATTN:Millicent Garland NOTE: Read instructions before completing this form.
PARAMETER *. /QUANTITY OR LOADING 1QUALITY j-EX OR CONCENTRATION NO. FREQUENCY SAMPLE OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE **** ***** ** **** 04***
CENTIGRADE MEASUREMENT 0 00010 I 0 PERMIT ********* *********** REPORT DEC C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous TEMPERATURE, WATER DEG. SAMPLE ***** **** *04* ****
CENTIGRADE MEASUREMENT 0 00010 Z 0 PERMIT ********** ********** 30.5 DEG C CONTIN CALCTO INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE *******04**
UPSTRM DEG.C MEASUREMENT 0 00016 1 0 PERMIT ********* ***** ***** 5 - DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous FLOW, IN CONDUIT OR THRU SAMPLE ********** *********
TREATMENT PLANT MEASUREMENT 03
- 50050 1 0 PERMIT ***** Req. Monl. MGD **************** CONTIN RCORDR EFFLUENT GROSS VALUE RQIENTDAILY MX UOUS CHLORINE, TOTAL RESIDUAL SAMPLE **********19****
MEASUREMENT 1 50060 1. 0 PERMIT ********** *****0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE ********** *********
CHANGE MEASUREMENT 04 **
82234 1 0 PERMIT *****2 DEG C ******** ********* *CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER - Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE
_____________________________ direction or supervision in accordance with a system designed to assure that qualified *.
- Christopher Schwarz personnel properly gather and evaluate the informaTion submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering it VcePe_ nt-43 4-70 15 12 0 the information, the information submitted is, to the best of my knowledge and belief, true,42 83-0 1 5 12 1 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
______________________________i nformation, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUBR YARM A TYPED OR PRINTED _________________CODE/
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
N._are TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMAR)
(*SUBR 01) OMB No. 2040-0004 A._ddre._ss P.O. BOX 2000 NTEROFFCE OPS-5N-SQN.)
.(I- F - FINAL SODDY - DAIS.Y, TN.._373._84__ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER F._acili~y TIVA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY [ ~MONITORING PERIOD EFFLUENT tYEARI MO T AYI IYER M DAY NO DISCHARGE jj ***
A-TTN:Millicent Garland Froml 15 11 01l Tol jjL 1 J ***
NOTE: Read instructions before completingl this torm.
PARAMETER QUANTITY OR LOADING 1QUALITY __ __
OR CONCENTRATION
_ _- __ _ _ __ _EX NO. FREQUENCY OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 1C25 STATRE 7DAY CHR SAMPLE ******** ******** ** *5*5*********
CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 PERMIT ******** ************ 42.8 ****** ***** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC26 STATRE 7DAY CHR SAMPL*E ************ ***** ****
PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT ******* ******** ***42.8 ********* PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT
,REQU1REMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT' REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMITr REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. eased on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best ot my knowledge and belief, true, 423 843-7001 15 12 10 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 'YEAR MO DAY AREA NUMBER TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01 ) 0MB No. 2040-0004 Address P.O. BOX 2000
- . INTEROFFICE OPS-5N-SQN)
I TN0026450 II118 G ]F-FINAL SODDY - DAISYTN 37384 I PERMIT NUMBER I[DISCHARGE NUMBER] WASTEWATER & STORM WATER Faciliy. TA - SEQ UOYAH NUCLEAR PLANT Location HAMILTON COUNTY MflNITflPIN* PlPCfIfl 1 EFFLUENT From 15 111 101 Tol 15 11 3o -*NOSHAGE ATTN:Millicent Garland NOTE: Read instructions before completingq this form.
PARAMETER QUANTITY OR LOADING
________IEX IQUALITY OR CONCENTRATION NO. FREQUENCY OF SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ********************
MEASUREMENT 19 00300 1 0 PERMIT ********* **2 ***** **** MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM _______WEEK SOLIDS, TOTAL SUSPENDED SAMPLE *******************
MEASUREMENT 19 00530 1 D PERMIT **,* **** .. **** ******10 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE ********** *25* ****
MEASUREMENT 2
!00545 1 0 PERMIT ********* ** ******** ML/L ONCEI GRAB EFFLUENT GROSS REQUIREMENT" DAILY MX. MONTH FLOW, IN CONDUIT OR THRU SAMPLE ***** ****
TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. Req. Monl. MGD ***************ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MOAG DIY XBTC SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER
~~lCertify ire~~~~d under penalty of law that this document and all attachmants were prepared under my TELEPHONE DATE Christopher Schwarz
- ction or supervision in acco rdan ce with a system designed to assure. t hat qualified*..
personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persona who manage the system, or those persons directly responsible for gathering 6
- t .. *n L
it Vice P_ t__42_83701_5 12 1 President Site Vice ~the information, the information submitted is , to the best of my knowledge and belief, true,Pesen42 83-01 5 12 0 SiePesdetaccurate, ic and complete. l am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
- information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CD COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period
Attachment 1 Flow in Conduit or Through Treatment Plant -
Monthly Average Flow Month Monthly Average Flow (MGD)
June 1757 July 1788 August 1788 September 1788 October 1803
TVA Sequoyah Nuclear Plant NPDES Permit No. TN0026450 Attachment 2 Turbine Building Sump Monitoring Data During November 2015, the turbine building sump was discharged directly to the yard drainage pond from 11/20/2015 to 11/27/2015. During this period, the turbine building sump was monitored in accordance with the narrative condition found in Part 1A.2 of NPDES Permit TN0026450.
Parameter Daily Minimum Monthly Daily No. of Samples
__________________ Average Maximum Flow 1.689 2.145 2 pH8.05 s.u. - 8.59 s.u. 2 O&G -<5.0 mg/L <5.0 mg/L 2 TSS -<13.5 mg/L 22.0Omg/L 2
Attachment 3 Toxicity Testing October 18 - 23, 2015 Final Report
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: November 16, 2015
- 1. Facility / Discharger: Sequovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450 4: Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): L257
- 7. 1Q10:3,9
- 8. OutfallTested: 1.01
- 9. Dates Sampled: October 18-23,2015
- 10. Average Flow on Days Sampled (MGD): 1794. 1825, 1801 11I. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: October 20 - 27, 2015
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids ( Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101" 10.7, 21.4, 42.8, 85.6. 100 Intake: 10__0_
Pimephalespromelas: UV treated Outfall 101: 10.7, 21.4, 42.8, 85.6. 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC25 = 42.8%
- 17. Test Results: Outfall 101: Pimephalespromelas."IC 2 5 > 100%
Ceriodaphniadubia: IC 2 5 > 100%
UV treated Outfall 101" Pimephalespromnelas. IC 2 5 > 100%
Page 1 of 98
- 18. Facility
Contact:
Millicent Garland Phn#:(2)8361
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone Ii: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Exposures to samples collected October 18 -23, 2015 from Outfall 101 resulted in no toxic effects to daphnids. Minnow survival was significantly reduced in minnows exposed to the full-strength effluent. Pathogenic growth was also observed in the gill area of minnows in the full-strength effluent.
The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids and minnows to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival Was improved by two
- minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference may have resulted in the minnow mortality observed in the full-strength effluent.
Page 2 of 98
METHODS SULMMARY Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)
Sample Time (ET) Time (ET) Temp. TRC* Time (ET)
ID Collected Received (°C (rag/L) Last Used By 101 10-18-15 0645to 101-512 .,.1 <.0 10-20-15 1155 10-19-15 0545 1-91125 .819t <10 10-21-15 1100 Inae 10-18-15 0715 to 1-91125.6 <10 10-20-15 1155 Inae 10-19-15 0615 1-95125.6 <10 10-21-15 1100 10-20-15 0706 to 10-21-15 1230 .A._1,9 <0.10 102-506 11 10-21-15 0606 10-23-15 1055 Inae 10-20-15 0730 to 1--5120.6 <.1 10-22-15.15 Inae 10-21-15 0630 1--5120.6 <10 10-23-15 1055 0707 to10-24-15 1057 101 .10-22-15 0770 10-23-15 1250 2 . 4 , 1 ,4 * <0.10 1.0-25-15 1055 10-23-15 0607 10-26-15 1055 10-24-15 1057 Intake 10-22-15,0728 to 10-23-15 1250 0.8 <0.10 10-25-15 1055 10-23-15 0628 10-26-15 1055
- TRC =Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainer's. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: .Samples from Outfall 101 and intake were warmed to test temperature (25.0+/-+1.00 C) in awarm water bath.
Aliqiuots of Outfall 101 and Intake samples were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aciuatics.
Inc.) for 2 minutes.
Page 3 of 98
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: In-house Cultures Aqiuatox, Inc.
- 2. Age: 18.70 - 19.92 hours0.00106 days <br />0.0256 hours <br />1.521164e-4 weeks <br />3.5006e-5 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static. Renewal Static. Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 _1
- 6. Test Initiation: (Date/Time)
Outfall 101 10-20-15 1155 ET 10-20-15 1000QET UV Treated Outfall 101 10-20-15 1142 ET
- 7. Test Termination: (Date/Time)
Outfall 101 10-27-15 1113 ET 10-27-15 0904 ET UV Treated Outfall 101 10-27-15 1052 ET Mean = 24.9°C
- 8. Test Temperature: Outfall 101: Mean = 24.7°C (24.2 - 25.2°C) (24.5 - 25.30 C)
Test Temperature: UV-Treated Outfall 101 : dean = 24.8°C 24.2 - 25.3°C)
(C
- 9. Physical / Chemical Measurements: Alkalinity, hardness. total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinneyville. CA).
Page 4 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench .Sheets)
- 1. Results of a Pimephalespromelas Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20--27. 2015 using effluent from Outfall 101.
Test Percent Surviving Solutions time ihterval
(___ used
[ .1 [4 1 I
____ -days)
(% Effluent) 1 2 3 5 [6 7 Control 100 -100 100- 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 98 98 98 85.6% 100 100 100 100 100 100 100 100.0% 100 100 98 93 83 78 75 Intake 100 100 100 98 98 98 95 Mean Dry Weight (mag)
Test Solutions (replicate number)______
(% Effluent) ] 2 [ 3 [ 4 Mean
- Control 0.616 0.492 0.528 0.579 0.554 10.7% 0.597 0.612 0.632 0.551 0.598 21.4% 0.618 0.538 0.559 0.644 0.590 42.8% 0.660 0.609 0.633 0.547 0.612 85.6% 0.617 0.624 0.588 0.633 0.616 100.0% 0.693 0.318 0.430 0.606 0.512 Intake 0.509 0.557 0.737 0.461 0.566 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: N__A_
Lower Limit: NA________ _______
- TUa = 100/LC5 0 : TUc =100/IC25 Page 5 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20 -27. 2015 using effluent from Outfall 101.
Percent Surviving Test (time interval used -days)
Solutions
(% Effluent) 1 2 3 4 5 67 Control ioo 100 100 100 10o 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8% 100 100 100 100 100 100 100 85.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 TestSoluionsReproduction (#young/female/7 days)
Test Solutions~Data
(% Effluent) 1if 2 J 3 [ 41.5_16 ]
(replicate number)__
7 8 r _____
9 ]. loMean Control 30 29 29 30 30 31 31 31 32 28 30.1 10.7% 33 31 30 30 30 32 30 32 35 32 31.5 21.4% 33 32 34 31 35 31 33 34 32 32 32.7 42.8% 32 36 32 34 34 33 33 34 31 33 33.2 85.6% 33 38 37 32 35 37 33 33 33 36 34.7 100.0% 36 34 39 38 32 39 35 36 37 36 36.2 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 42.8%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA _______________
- TUa = 100/LC5 0 : TUc =100/IC25 Page 6 of 98
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic!/7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted October 20 - 27. 2015 using water from Intake
~Percent Surviving Test ____ time interval
(___ used -_days)____ ____
Solutions 1 1
(%Efun) 112 3[ 4 516 7 Control 100 100 100 100 100 '100 100
- Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Data (replicate number)
(%Effluent) 1 2 3 I4 I 5 6 [7 I 8 9 10 I[Mean Conrol 30 2830 29 29 32 29 33 32 3.0k 30.2 Intake 135 34 34 33 36 34 31 32 31 32 33.2 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:.
Upper Limit: NA
- Ta=Lower Limit: N__A __________________
- Ta=100/LC 5 0 : TUc = 100/IC 25 Page 7 of 98
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic!/7-day Toxicity Test.
- (Genus species) (Type / Duration)
Conducted October 20 -27,.2015 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions interval used -_days)____
(____time
(%Effluent) 1 ] 2 ... 3 [ 4..] 5 ... J 6 7 .
Control ioo 100 100 100 100 100 100 10.7% 100 100 100 100 100 100 100 21.4% 100 100 100 100 100 100 100 42.8%. 100 100 100 100 100 100 100 85.6% 100. 100 100 100. 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 TestSoluionsMean Dry Weight (mg) number)
(%Effluent) 1
______ ________(replicate 2_ ] 3 _4. . Mean Control 0.593 0.600 0.618 0.512 0.581 10.7% 0.542 0.695 0.566 0.558 0.590 21.4% 0.655 0.573 0.597 0.599* 0.606 42.8% 0.647 0.617 0.494 0.670 0.607 85.6% 0.559 0.614 0.568 0.623 0.591 100.0% 0.613 0.609 0.677 0.573 0.618 Intake 0.603 0.531 0.547 0.591 0.568
- IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: N__A Lower Limit: N__A
- TUa =100/LC5 0 : TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) ___
Species__ Date J Time [Duration Toxicant Results (IC25 )
Pimephalespromelas October 20 - 27, 2015 1130 7-days KC1 0.72 g/L Ceriodaphniadubia October 06 - 13, 2015 1030 7-days NaCI 1.06 g/L Page 8 of 98
Na PHXSICAL/CHEMICAL
SUMMARY
Water Chernistry Mean Values and Ranges forPimephalespromelasand Ceriodaphniadubia Tests, Non-treated SequoyahNuclear Plant (SQN) Out1*1 101 Co performed October 20-27, 2015.
0 Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual 0
____ Initial Final Initial Final Initial Final (pnmhos/cm) (mg/L CaCO3 ) (zng/L CaCO3 ) Chlorine (mg/L)
(D Cotl 24.8 24.5 7.7 7.6 8.02 7.81 317 63 88 24.7 - 24.9 24.3 - 24.8 7.5 - 7.9 7.0 - 7.8 7.95 - 8.13 7.66 - 7.94 302 - 335 62 - 64 88 - 88 1.% 24.9 24.5 .7.8 7.5 8.02 7.79 305 24.7 - 25.0 24.4 - 24.6 7.5 - 8.0 6.5 - 7.9 7.97 - 8.14 7.61 - 7.94 291 - 318 _ _____________
216% 24.9 24.5 7.8 7.5 8.03 7.79 288 24.7 - 25.0 24.2 - 24.6 7.6 - 8.0 6.2 - 7.9 7.98 - 8.15 7.58 - 7.94 280 - 296 _ ____ ______
4.% 24.9 24.5 7.8 .7.5 8.03 7.78 258 24.7 - 25.0 24.4 - 24.7 7.6 - 8.0 6.0 - 7.9 7.98 - 8.13 7.55 - 7.92 251 - 267 ______
86.4% 24.9 24.6 7.8 7.5 8.02 7.78 201
- g.24.8 - 25.1 24.4 - 24.7 7.6 - 8.0 6.0 - 7.9 7.95"- 8.06I 7.52 - 7.92 191 - 211 _ ____ _______________
10.% 25.0 24.6 7.9 7.6 8.00 7.79 181 72 77 < 0.10 24.8 - 25.1 24.4 - 24.7 7.6 - 8.0 6.6 - 8.0 7.89 - 8.07 7.55 - 7.94 170 - 189 69 - 73 69 - 90 <0.10 - <0.10 Inae 24.9 24.5 7.8 7.6 8.02 7.81 181 70 68 <0.10
_____24.7 - 25.2 24.4 - 24.6 7.6 - 8.0 6.8 - 7.9 7.93 - 8.09 7.62 - 7.97 172 - 191 67 - 71 61 - 73 <0.10 - <0.10 Coti 24.8 25.0 7.7 7.9 8.02 7.98 317 63 88 24.7 - 24.9 24.5 - 25.2 7.5 - 7.9 7.7 - 7.9 7.95 - 8.13 7.92 - 8.07 302 - 335 62 - 64 88 - 88 _________
1.% 24.9 24.9 7.8 7.8 8.02 7.99 305 24.7 - 25.0 24.5 - 25.2 7.5 - 8.0 7.8 - 7.9 7.97 - 8.14 7.92 - 8.07 291 - 318 21.6% 24.9 25.0 7.8 7.9 8.03 7.99 288 24.7 - 25.0 24.6 - 25.3 7.5 - 8.0 7.8 - 7.9 7.98 - 8.15 7.92 - 8.06 280 - 296 _ ____
4".2% 24.9 24.9 7.8 7.9 8.03 8.00 258 24.7 - 25.0 24.5 - 25.2 7.6 - 8.0 7.8 - 8.0 7.98 - 8.13 7.93 - 8.09 251 - 267 _ ____
24.9 25.0 7.8 7.9 8.02 8.01 201 86.4%/
24.7 - 25.0 24.8 - 25.1 7.6 - 8.0 7.8 - 8.0 7.95 - 8.06 7.94 - 8.09 191 - 211 _____ _________
10.% 25.0 25.0 7.9 7.8 8.00 8.01 181 72 77 <0.10 24.7 - 25.1 24.9 - 25.2 7.6 - 8.0 7.6 - 7.9 7.89 - 8.07 7.94 - 8.09 170 - 189 69 - 73 69 - 90 <0.10 - <0.10 Inae 24.9 24.9 7.8 7.8 8.02 8.03 181 70 68 <0.10
_____ _____24.7 - 25.0 24.7 - 25.0 7.6 - 8.0 7.7 - 8.0 7.93 - 8.09 7.96 - 8.14 172 - 191 67 - 71 61 - 73 <0.10 - <0.10 Overall temperature ("C) Average Minimum Maximum Pimephalespromelas ,24.7 24.2 25.2 Ceriodaphniadubia 24.9 24.5 25.3
In PHYSICAI/CIIEMICAL
SUMMARY
Water Chemistry Mean Vabies and Ranges for Pimephalespromelas Test, UV-treated S equoyahi Nuclear Plant (SQN) Outfl~sl 101 perforined October"20-27, 2015.
0 0 Test Sample ID) Temperature (°C) Dissolved Oxygen (nag/L) pH (S.U.) Conductance Alkalinity Hardness *Total Residual
-Og ID _____Initial Final Initial Final Initial Final (pumhos/cm) (mg/L CaCO3 ) (mg/L CaCO 3) Chlorine (mg/L)
Co Cotl 24.9 .24.5 7.8 7.5 8.02 7.81 316 63 86 24.8 - 24.9 24.4 - 24.7 7.7 - 7.9 6.7 - 7.9 7.95 - 8.12 7.64 - 7.95 302 - 328 63 - 63 86 - 86 _______
108% 24.9 24.5 7.8 7.5 8.02 7.80 303 24.8 - 25.0 24.3 - 24.7 7.8 - 7.9 6.7 - 7.9 7.96 - 8.13 7.60 - 7.93 291 - 312 ______
26% 25.0 24.6 7.9 7.6 8.03 7.81 288
,24.9 - 25.1 24.3 -'24.7 7.8 - 7.9 6.7 - 7.9 7.96 - 8.14 7.61 - 7.93 278 - 298 ______
42%
_____24.9 25.0
- 25.1 24.224.5
- 24.7 7.8 7.9
- 7.9 .7.5 6.0 - 7.9 7.97 8.03.
- 8.15 7.53 7.79
- 7.92 251 260
- 275 _____ ______
86.4% 25.0 24.5 7.9 7.4 8.04 7.78 204 86"4___ 24.9 - 25.1 24.4 - 24.7 7.8 - 8.0 6.0 - 7.9 7.98 - 8.15 7.54 - 7.95 197 - 213 _______ ______
10.% 25.1 24.4 7.9 7.6 8.04 7.79 .184 72 73 < 0.10
_____25.0 - 25.3 24.3 - 24.7 7.8 - 8.0 6.8 - 7.9 7.97 - 8.16 7.53 - 7.96 174 - 193 71 - 73 69 - 82 <0.10 - <0.10 Inae 25.1 24.5 7.9 7.6 8.04 7.86 182 71 65 < 0.10
______ ____25.0 - 25.2 24.4 - 24.6 7.8 - 8.0 6.8 - 7.9 7.97 - 8.17 7.71 - 7.97 174 - 194 69 - 73 61 - 69 <0.10 - <0.10
- Note: Total residual chlorine was performed on non-treated.Outfall 101 and Intake samples.
Overall temperature (*C) Average Minimum Maximum Pimephalespromelas 24.8 24.2 . 25.3
SUMMARY
/ CONCLUSIONS Exposures to samples collected October 18- -23, 2015 from Outfall 101 resulted in no toxic effects to daphnids. Minnow survival was significantly reduced in minnows exposed to the full-strength effluent. Pathogenic growth was also observed in the gill area of minnows in the full-strength effluent.
The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids and minnows to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference may have resulted in the minnow mortality observed in the full-strength effluent.
Page 11 of 98
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimnephalespromnelas Tests were conducted according to EPA-821I-R-02-0 13 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-82 l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimeyhales promnelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphniadubia None Page 12 of 98
PHYSICAL AND CHEMICAL METHODS*
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B-2000.
- 5. The pH was measured by SM 4500-H+ B-2000.
- 6. Conductance was measured by SM 2510 13-1997.
- 7. Alkalinity was measured by SM 2320 B-1997.
- 8. Total hardness was measured by SM 2340 C-1997.
- 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaCI.
Sodium Chloride (NaC1 crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 98
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater. 22 "dEdition, 201l2.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 98
Sequoyah Nuclear Plant Bijomonitoring October 20 - 27, 2015 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 98
Table B-I1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco Hl-150M Hypochlorite mg/L mg/L mg/L mg/L mgiL mg/ 73551 mgiL mg/L TRC Phosphate Copolymer DMAD Azole Quat mgiL Quat TRC ____ _ _ _ _ _ ____EOiPO 02/06/2005 - <0.0042 0.028 0.010 - --
02/07/2005 , <0.0116 0.028 0.010 - - - 0.007 -
02/08/2005 , <0.0080 0.028 0.010 -....
02/09/2005 , 0.0199 0.028 0.010 - --
02/10/2005 - <0.0042 0.028 0.010 ----
02/11/2005 -0.0155 0.028 0.010 - - 0.007 06/05/2005 -0.0063 .... --
06/06/2005 0.0043--- - - - 0.037 06/07/2005 0.0103--- - - - 0.037 06/08/2005 - 0.0295-- - - - 0.037 06/09/2005 , 0.0129 - - ---
06/10/2005 - 0.0184 - ----
07/17/2005 - 0.0109 0.026 0.009 -- -
07/18/2005 - 0.0150 0.026 0.009-- - - 0.036 07/19/2005 - 0.0163 0.026 0.009-- - - 0.036 07/20/2005 -0.0209 0.026 0.009 - -- 0.014 0.036 07/21/2005 0.0242 0.026 0.009 - - --
07/22/2005 -0.0238 0.054 0.018 - - - 0.014-10/30/2005 - 0.0068 - - -- -
10/31/2005 - 0.0112 - - ----
11/01/2005 - 0.0104 --- - - 0.035 11/02/2005 0.0104-- - - - 0.036 11/03/2005 - 0.0117 - -- - - 0.036 11/04/2005 - 0.0165 - - - 0.035 11/14/2005 - 0.0274 - - - I 11/15/2005 - 0.0256 .....--
11/16/2005 - 0.0234 .... --
11/17/2005 - 0.0231 - - - -,
11il18/2005 - 0.0200 - -- - --
11/19/2005 0.01 16 - - - --
Page 16 of 98
Table B-i. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbronm PCL-222 PCL-401 CL-363 ... Cuprostat-PF H-130M Nalco H'150M MSW Hypochlorite mgiL mg/L mg/L mg/ mg/L mg/b 73551 mg/b 101 mg/b TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC _______ ____EOiPO Phosphate 11/12/2006 - 0.0055 ......... -
11/13/2006 - 0.0068 - , - - - 0.037-11/14/2006 - 0.0143 - , - - - 0.037-11/15/2006 - 0.0068 - - - - - 0.037 11 /16/2006 - 0.0267 - -- - - 0.037 11/17/2006 - 0.0222 ----- --
11/26/2006 - 0.0188 - - --- --
11/27/2006 - 0.0138 - - ------
11/28/2006 -0.0120 -------
11/29/2006 -0.0288------ -
11/30/2006 -0.0376 -------
12/01/2006 -0.0187 -------
05/28/07 -------- 0.015 05/29/07 ------- 0.036 0.015 05/30/07 -0.0084 -- -- 0.017 0.036 0.015 05/31/07 -0.0103 ----- 0.036 0.015 06/01/07 -0.0164 --- - 0.017 0.036 0.015 06/02/07 -0.0305 --- -- 0.015 12/02/07 -0.0241-- -----
12/03/07 -0.0128 -------
12/04/07 -0.0238- ------
12/05/07- 0.0158 ------ -
12/06/07 -0.0162--------
12/07/07 -0.0175- -----
04/13/08 -0.0039--------
04/14/08 0.01t24 - ------
04/15/08 0.0229 - -------
04/16/08- 0.0143 - - -- ---
04/17/08 0.0120 - - ---- -
04/18/08- 0.0149 - ----- -
10/26/08 -0.0260--------
10/27/08 -0.0151 ----- 0.017 --
10/28/08 - 0.0172 ----- - 0.041-10/29/08 - 0.0154 --- - - 0.018 0.041 0.030 10/30/08 ......- -- 0.041 0.030 10/31/08 - 0.0086 --- - - - 0.041 0.030 Page 17 of 98
Table B-i1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL- PCL-401I CL-363 Cuprostat H-130M Nalco iSpectrus H-i150M MSW Hypochiorite mg/L 222 mg/L mg/b -PF mgiL mg/b 73551 CT1300 mg/b 101 mg/ TRC mg/b Copolymer DMAD Azole Quat mg/L img/ Quat mg/b TRC Phosph EO/PO Quat Phosphate 02/08/09 ... ... 0.0197 - - - - 0.017--
02/09/09 - 0.0237 , - - 0.0 17 ---
02/10/09 ... - 0.0104 - - - - 0.021 . . . ,
02/1 1/09 -0.0155 -- -- 0.017 --
02/12/09 ' 0.0106 - --- - 0.017 --
02/13/09 - - - - ----
05/10/09 - 0.0129 - -- - --
05/t11/09 - 0.0415 - ----- - 0.0446 05/12/09 - 0.0053 - - --- - 0.0396 05/13/09 . 0.0049 - ----- - 0.0396-05/14/09 - <0.0141 - ----- - 0.0397 05/15/09 - <0.0160 ....- --
11/15/09 - 0.025 ------ --
11/16/09 - 0.0152 --------
11/17/09 - 0.0255 ---- ----
11/18/09 - 0.0306 ---- ----
11/19/09 - 0.0204 --------
11/20/09 - 0.0093 -- ------
05/09/10 - 0.0192 - - ----- -
05/10/10 - 0.0055 - - -- ----
05/11/10 - 0.0100 - ---- - 0.039 -
05/12/10 - 0.0171 - ---- - 0.039 --
05/13/10 - 0.0041 - --- - 0.039 --
05/14/10 - 0.0099 - --- - 0.039 --
Page 18 of 98
Table B-i. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbron PCL- PCL-401 CL,363 Cuprostat H-130M Nalco Spectrus H-I150M MSW Floguard Hypo- mgiL 222 mgiL mg/b -PF mg/b mg/b 73551 CT1300 mgiL 101 MS6236 chlorite TRC mg/ Copoly- DMAD Azole Quat mg/L mg/b Quat mg/b mgiL mg/b Phos- mer EO/PO Quat Phos- Phosphate TRC phate ____________phate ____
10/31/10 - - - ..... -- ,
11/01/10 - 0.0122 - - -- -----
11/02/10 - 0.0112 - ....- --
11/03/10 - 0.0163 - - --------
11/04/10 - 0.0107 --- ------
11/05/10 - 0.0132 ---------
05/01/2011 ....-- ---
05/02/2011 - ------ - 0.04 ---
05/03/2011 - - ---- - 0.04 ---
05/04/2011 - 0.0155 ----- - 0.04 - -
05/05/20 11 - 0.0 179 - -- - 0.04 ---
05/06/2011 - 0.0089 - - -- -----
11/06/2011 - 0.0168 - - -- -----
11/07/2011 - 0.0225 - - --- ---
11/08/2011 - 0.0141 ....- --
11/09/2011 - 0.0239 ....- --
11/10/2011 - 0.0242 - ---------
11/11/2011 - 0.0231 - ---------
05/06/20 12 -....- ---
05/07/20 12 -....- ---
05/08/2012 -..... -- 0.041 ---
05/09/2012 - 0.0145 ----- - 0.041 ---
05/10/201!2 - 0.0298 ----- - 0.041 ---
05/11/2012 - 0.0174 - --- -----
08/12/2012 ...... -- -- 0.029 08/13/20 12 - 0.0256 - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 - - - --- 0.037 - - 0.029 08/15/2012 - 0.0279 -- -- 0.028 -- - 0.029 08/16/2012 - 0.0076 -------- - 0.029 08/17/2012 - 0.0446 -------- - 0.032 05/12/2013 - 0.0099 ....- --
05/13/2013 -.... -- 0.064 05/14/2013 - 0.0091 -- - - 0.039 -- - 0.064 05/15/2013 - 0.0096 , - 0.039 -- - 0.064 05/16/2013 - 0.0229 - ------- - 0.032 05/17/2013 - 0.0063 - - ----- 0.032 09/15/2013 ....-- --- 0.03 09/16/2013 - 0.0072 - - -- - 0.0379 - - 0.03 09/17/2013i - 0.0107 -- - - 0.036 0.0379 - - 0.03 09/18/2013 - 0.0217 ----- 0.036 0.0379 -- 0.03 09/19/2013 - 0.0172 -- ----- - 0.03 09/20/2013 - 0.0173 ---- --- 0.03 Page 19 of 98
Table B-1. Sequoyah Nuclear Plant Outfall 101 Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologicaily Induced Corrosion and Mollusks, During Toxicity Test Sampling, February 6, 2005 - October 23, 2015 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H,130M Nalco Spectrus H-150M MSW Floguard Hypo mg/L 222 mg/L mg/ -PF mg/L mg/ 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mgiL mg/ Quat mgt mg/L mg/ Phos- mer EO/PO Quat Phos- Phosphate
_____ TRC ____ hat ___e____ ____phate ____
05/04/2014 0.0118 - - --- ,
05/05/2014 - 0.01 12 - - - - --- --
05/06/20 14 - 0.0096 - - , --
05/07/2014 - 0.0164 ....- --
05/08/2014 0.0235 - - -------
05/09/20 14 0.0110 - - ------ -
09/07/20 14 ....-- ---
09/08/20 14 --- - - -- 0.04 - --
09/09/2014 -- --- - - 0.04 - -
09/10/2014 --- ---- - 0.04 -- -
09/11/2014 - 0.0070 ....- --
09/12/2014 - 0.0074 ....- --
08/09/2015-08/10/2015 0.0195 0.03 08/11/2015 0.0275 0.03 08/12/2015 0.0213 -0.03 08/13/2015 0.0192 -0.03 08/14/2015 0.0182 0.03 10/18/2015 0.0162 10/19/2015 0.0125 10/20/2015 0.0120 10/21/2015 0.0130 10/22/2015 0.0174 10/23/2015 0.0156 Page 20 of 98
Sequoyah Nuclear Plant Biomonitoring October 20 - 27, 2015 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 21 of 98
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1_of 1
- ient: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
l~oject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx LUPS Bus Client
-0. Number: N/A Asheville, NC Other ~
- cility Sampled: Sequoyah NP 28801 General Commen~ 5:
INPDES Number: TN0026450 Phone: 828-350-9364 Fax: 828-350-9368 U- U - U U Xbr~~. ,W~44% C.~4t~ CbIIeCAV~4'14 14+ ~.
Flow Collection Date/Time Container Number &
Volume (MGD) Rain Event?
(Mark as Appropriate)
- ,P~V~M 1~,
borat~zyt~
I 11111 V'-~A ~T~'-~
Yes If Yes, No Trace ETSLog. ArrivalTeizip. By. ~Tim~ App~ar~
Inches $umbcr (CC) I End
__ 1__ __
I'=!
L ~L.'gaI) 4:
/ 799. zr-SQN-TNT-TOX Comp
,__ I 7/5
,"-/'q-I5
~ *t4/*'4,.,i^.
(. gal) NA Ij:,** ' .
__________________________Sample Custody - Fill In From Top Down _______________________.__
Relinquished By (Signature): Date/Time Received By (Signature): G~* .t -
- a*~*XDate/Timel*tk. '~.
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnighit on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Whole Effluent Sample Receipt Log Page 4i¶*
f7~i~
w
~II" Date Tim Received Received "*Sample Project SampleSapenmandscllo
- Sample temperaure performed using Samlple Receiving~Thenmomnter2 State Comments SN 130580151 (D
10-19-15 1225 1.Sumner VA Courier 1.8* 1.9 10958 151019 .01 TVA -e uoyh Nudear Plant -101 TN 10-19-15 ... 1225 .......
LSumner VA CourIer 1.6 10958 "151019 .02 VA - Seuoyh Nudear Plant - INT TN 0
-em SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I
- hent: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client
.O. Number: N/A Asheville, NC Other seiy:.* l.. e 'e/*
~cility Sampled: Sequoyah NP 28801 General Comments:
Phone: 828-350-9364 I1thl Mz.v-*l,6 *,rt%U) s~4i, ,.,,',,,,o,..e.,,
O,,3 NPDES Number: TN0026450 Fax: 828-3 50-93 68
_______ _______ ____ ___ h - ------- --- -
Collection Date/Time Container FlowI Number & (MGD) Rain Event?
I Volume Yes (Mark as Appropriate)
If Yes, Inches No Trace tT~Log ~
tZor~tory~Jse Amv4 Temp.
c~Cy By ~Appear End
/0-ZO- /$~ ~o-ZI-l~5
~) L,~IoE*+
I I - -~ -- I I 1 ¶ T -.
SQN-1NT-TOX [Comp /O-~D ~ '0-74-Is- 1 (2.5 gal) NA I llI -ll I
£2 73'o
~ I i~Ip? O~
- - - - I I - I - I - .. .... ...... . I .... I -1.. "
Sample Custody - Fill In From Top Down Reli.nquished By (Signature): Date/Time Received By (Signature):
I 6~S
___________________________________________________________________ I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing Within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they' will not arrive until the following Monday.
Page T Whole Effluent Sample Receipt Log
'SniI teoeatr cefo, e usnvni Rec n lhro . e N 1... 8.1. 1 lime Received Received *Sample Project Sample i....
Date- 0 Received . Received by from Temp. ( C) number .number Sample name and dtescription State Comments
- ~- -I 10-21-15 0950 K.Keenan Fed - Ex 1.8 10968 "I151021 .01 ALCOA - 005 .. _____N__ ........ ....... ___
U' 10-21-15 09,50 K.Keenan Fed - Ex. 1.8 10968 151021 .02 ALCOA -012 _________ _NC________
10-21-15 0950 K.Keenan Fed - Ex 2,6 10969 151021 .03 SmithfieldPacking _____NC___ .....
0 10-21-15 0950 K.Keenan Fed - Ex 1,8 10970 151021 .04 ALCOA - 013 ____________' NC________
10-21-15 0950 K(.Keenan Fed - Ex 2.0 10971" 151021 .05 Dswberry - Regen - Grab ,_____"'__ ____________"_
10-21-15 0950 K.Keenan Fed - Ex .. ,2.0 '10971 151021 .06 Dewberry- Effluent.- Grab) ________ NC ___________
10-21-15 0950 K.Keenan Fed - Ex 2.6 10972 151021 .07 Beaufort - Glenda Drive WTP ___________ NC _________
10-21-15 0950 K.Keenan Fed - Ex 2.6 10973 151021 .08 Dixon WTP ________NC_____
10J-21-15 0950 K.Keenan Fed - Ex 4.2 10974 151021:.09 Dare County WTP ;N 10-21-15 0950 K.Keenan Fed - Ex 4.2 10975 15102,1..10 RWS. ________ _NC________
10-21-15 0950 K.Keenan Fed - Ex 4.2 10976 151021 .1 1 Skyco W*TP ___________ NC _________
10-21-15 0950 " K.Keenan Fed - Ex 4.2 10977 151021 .12 Stumpyv Point WTP ____ ___________
10-21-15 -0950 K.Keenan Fed - Ex 1.2 10978 15102,1..13 New Hanover WTP ______NC____
10-21-15 0950 K...K Keenan Fed - Ex 1.6 10979 - 151021 .14 Richland Township WTP _____NC__
10-21-15 1025 J. Sumner UPS 1.0 10980 151021 .15 Atlantic Be.ach WWTP ____N 10-21-15 1025 J. Sumner UPS 0.9 . 10981 15102.1, .16 Camden County.WTP _________ NC 10-21415 . 1230 J.Sumner W Courier TA .1.0/0.9 .10958 151021.,.17. TVA - Seq uoyah Nuclear Pla~nt* 101 TN J 10-21-15 .1230 J. Sumner
- TA Courier 1.6 10958 151021 .A8 TA - Sequ-_yah Nuclear Plant- Intak.e TN 10-21-15 1540 J. Sumner. TVA Courier 1.8 10982 151021 .19 TVA -JOF ME2C- Simulated Effluent TN 10-21-15 . 15,40 ,J. Sumner TWA Courier 2.0 10982 151021 .20 . VA-JOF ME2C- Upstream "TN SOP G4 - Exhibit G4.2, revision 0 1-03-12
SBIOMONITORING CHAIN OF CUSTODY RECORDPae1oI_
w (A~ient: TVA Delivered By (Circle One):
Environmental Testing Solution, Inc.
rn-oject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client
=?O. Number..N/A Asheville, NC
.Other(seiY: 'i, b !;*,'[
- cility sampled: Sequoya1 NP 28801 G~eneral Comments:** -
%Ag, .%epS',*, 4'%"? i 6cr,tx.,,P'f o"/z--<
NPDES Numnber:. TN0026450 Phone: 828--350-9364 Fax: 828-350-9368
~dB:
p a ~- I-~ a n i i n m n n B1 I I Container Number & ~?2ItA4) Q44 1 ' I D~
Grab/Comp. Rain Event?
Sample Description Collection Date-/rIime Volume Laborate ry Use I
&~' c~. ~~o- %?
~--i~-~s .
Instructions: Clients should fiil in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample
- is36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page O Whole Effluent Sample Receipt Log
.5 *SampIetempe~um peiformed usingSanip!e ReceMncThermometer SN230550151 RI (a . Date
- Received lime Received Received by Received from
- Sample Temp. (0C
.Project number Sample number Smlnm S e..m n *fpimSaeCmet an dtsripinStt om e 0
10-23-15 0950 J,. Sumner Fed - Ex 1.3 10968 '151023 .01 ALCOA - 005 Nc ___________
R.J 10-23-15 0950) J. Sumner Fed - Ex 1.3 10968 151023 .02 ALCOA -012 NC._.N_________
0 10-23-15 0950 J.1.Sumner Fed - Ex 1.4 10969 151023 .03 Smithfield Packing iNC___________
-Ii *10-23-15
- 1250 J. Sumner WVA Courier 2.4, 1.4 10958 151023 .04 TVA - Seq uoyah Nuclea~r..Plant -101 .... TN ___________
(0 10-23-15 1250 J. Sumner TWA Courier " 0.8 10958 .151023 .05 VA - Sequoyah Nuclear Plant - Intake ' TN ___________
SOP G4 - Exhibit G4.2, revision 01-03-12
___ /
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-8231-R-02-013 Method 1000.0)
Species: Pimephalespromeias Client: Tennessee Valley Authority County: Hamilton Facility: Sequovah Nuclear Plant Outfall: 10__1 NPDES #: ,TN0026450 Project #: *cC*
Dilutionprepara~tioninformation:
Dilution prep (%) 10.7 21.4 42.8 I85.6 100
.~ Comments:
I Diluent volume (mL) 2232.5 1965 J1430 I360 j 0 _______________I
- Totalvolume (..L.)., 2500 2500 2500 2500 *2500 Test organism information: ...... Test information: __.........
Organism age: . o%* * *.7 t 0* Randomi zin g template: _____._*.
Date and times organisms 10-19-15 1600-1700 Incubator number and were born between: ______________shelf location: ____
Organism source: ATOX Batch Pp: 10-19-15 Artemia CHM number: CHM836 Drying Infonnatlonfor weight
______________________deterimiation:____
Transfer bowl information: pH = t . S.U.' Date!/Time..in oven: ].%t
_________Temperature.= ". 0C .. Initial oven temperature: * '...0 Average transfer volume: 0.1749 rL .. :Date / Time out of oven: .,t Final oven temperature: ,o*
._______ ___________Total drying time: 'Z.*. 6.
1)ailyfeeding andrenewal information:
Day Date Morning feeding A~flernoon feeding "" Test initiation, Sample numbers used MHtSW renewal, or batch
________ ____ termination used Time
,,____ Analyst Time Analyst ... Time Analyst Outfall 101 I ... Intakce,,_
0s l0-20-15 _______
- I,.OO *L.... %o$% ' .. .Stb*.O\
% r*
- t I l o-*
6 10-26-15 o 11 At I e15
'7 10-27-15 "
Controlinformiation: ... Acceptance criteria *Summary of test en~dpoints:
% Mortality: 0,* 20% 7-day LCso ? irt, Average weight per initial 1arvae: or NOEC __________
Average weight .persurinvin'g. larvae: .... o Ž0 .2,5mgfl~ar'ae .... .OEC . *.
ChV IC25 ________
SOP AT20 - Exhibit AT20.3, revision 11I-01-I4 28 of. 98
- * . . . .P a g e 2 of 6 Species: Phmephales.promelas Client: TVA / Sepnovah Nuclear Plant. Outfall 101.,NON-trea~ted Date: 10-20-15 Survival and Growth Data ayCONTROL 17%21.4%-
______AI B C D E F G H I J K L 10 10 10 10 10 10. t0 "10 10 10 10 10
.. 4* 0 t D t O t D r O t 5 *t Ot O 0* 0* Ot 6 b e
- I b P * *
- I 6
- A= Pan weight (mag).
Tray color code: *".
Date: OQ-fl'- ,
B'= Pan + Larvae weight (rag)
C 'Larvae weight (mag)=fB - A ..
HAndalyusted *S li)t d~~' ~ ~ ~ A Analy.it:
Weight per initial nnnmber of larvae (mg)
= C / Initial number of larvae V I.
Hand calculated.
Analyst: . .
a'
~j~a
'0 .4. '0 C,
_.._I ..-
Averg egt ofper initial number Percent reduction from coatol (%) f , ' 4
- .- IS ~01 -. ~ *-
larvae (mg) I J Comment codes: c = clear, decanted d = dead, fg = Iun.gus, k =killed, m =missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = and returned, w ffwounded.
Pa e 29 of 98 SOP AT20 - Exhibit AT20.3, revision. 11-01-14
Page 3 Of 6 Species:. Pimephalespromelas Client: TVA / Sepuoyah Nuclear Plant, Outfall 101, NON-treated Date: 10-20-15
________________ ____________Surrival and Growth Data "_______
Dy42.8% 85.6% _ 100%
10 10 10 10 10 10 10 10 10 10 10 10 4 ~10Ib 16 / lO I'D 1O LO . 1Q 10 ,*,
40 A-= Pan weight (rag) .. .
Tray color code:: t Date: 0o -2*__-15 B= Pan + Larvae weight (rag) 591 '
Date: ______ -______"
C =Larvae weight (rag) =B - A Hand calculated. ~~~'A% ~ ~ A'U ~ ~ ~~
Analyst: t . ..
Weight per initial number of larvae (mng)
= C/IInitial number of larvae Analyst:. * " "
- J Average weight per Percent reduction initial nunmber of fromncontrol (%) 0 * *- -- 4 L0,
- i A o *,0*
- 0* ,*ol larvae (rag)_______ _____________ _ _____
Comment codes: c =clear, d = dead,.fg = fungus, k = killed, m = missing, sk =sick, sm = unusually small,
-lg= unusually large, d&r = decanted and returned, w = wounded.
Comments: -* P,*' ea &¢. Ca~*t2*%*.Jt.R.. ¢r._ A.e 0* l f I.d.p.nd.,t
~
1k- SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 30 of 98
Page 4 of 6 Species: Pimevhale$oromelas Client: TVA / SeiuoyahNuclear Plant, Outfal ,1,0,1. NON-treated Date: 1t)-20-15 Day 100% Intakff*me 0 0 0 1 10 2 10 0(0 O 6
A = Pan weight (mag)
Tray color code:: ...
Analyst: ... ' * (
Date: __ *_____-____
B = Pan + Larvajr weight (ing) - -
- Analyst: 'j ~ ~ tL Date: i*-&g-i5- . [*o C = Larvae~weight (mag) = B- A Hand calculated.. "*
Analyst:
Weight per initial number of larvae (mug)
C /Initial number of larvae
- Hand calculated. **
Analyst: . .* *o Avrg egtper Percent reduction initial number of [ from control (%) *
- 2,,o' larvae m ________________1________________
Comment codes: c --clear, d = dead, fg = fungus, k = killed, m = missing, sk =sick, sni- unusually small, Ig =unusually large, d&-r - decanted and returned, w = wounded.
Commeents:
- Page 31 of 98 SOP AT20 - Exhibit AT20.3, revision 11-01-14
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015
.5O .PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Ic Project numbern 10958 C~eeprlteal(tt) ReIwen ~lettull hu lrel" Flit nmetedt* A=Pan wdkt(m) B=Nal+Lm'en tJmnnawetlt(30), Wetshf~lL,ndele MznwelJel Caeleet~ri~vzt'tmltna Weg~lkt/ fJndttcmber Melan annex Meuxwghtf In~tfllafl Cedthtextef Peeseutndxleefmi A___ 10 "10 15.61 21.77 6.16 0.61'60.6 Control B 10 tO 16.01 20.93 . 4.92 0.492 0.5.54 9.9 0.492 100.0 0.554 9.9 Net applicatble C 10 10 1627 21.65 5.28 0.528 0.528 D 1_1_1.2_*2208 ,,5.9 _057 ,,,0.579 _____ ______ _____ _ ________
E . 0 10 16.16 22.13 5.97 0.597 0.597 G 10 10 15.77 22.09 6.32 0.632 056.80.612 10.7_ ______G10 _______
15.77_ __ 2.09___ 6.32____ 0.632 0.598 5.0.632 100.0 0.598 5.8 -8.0 H 10 10 16.36 21.87 ,5.51 0.55r - , _____ ________ 0.551 _____ ______
____ 10_____ ____0___ 15.08____ 23.26____ 6.8 060.618 214 01 5. 06 .8058050S40.3538 100.0 0,590 8.4 -6.5 1
!0 10 . 15.26 20.64 5.5890538 0590 .40.559 L IO 010 16.22 22.66 6.44 d.644 _______0.644.____________ ______
- 4. N 19 .22.36.9.77069920.609 97.5 0.612 7.9 -10.6 10 90 16.29 22.38 6.0930677 0.299 0.633 P . 10 10 10.06 21.53 5.47 0.547 0.547 ____________________
_a...Q 10 10 15.89 22.06 6.17 0,617 0.617 8.5.691 1K 10 10 15.91 22.15 6.24. 0.624 0.616 3.2 0.624 100.0 0.616 3.2 -11.2 S 10 10 16.12 22.00 5.88 0.588 0.5*88
_______ T 10 10 ". 16.81 23.14 6.33 0,633 _______ _______ 0.633 _ __ _____ ______ _______
U 10 9 . 16.47 23.41) 6.93 0.770 0,693 100% V 10 6 16.11 19.29 3.18 0.530 0.673 15.3 0.430 75.0 0.512 33.1 7.6 W 10 6 16.61 20.91 4.30 0.717 .0"
________ X 10 9 15.71 21.77 6.06. 0.673 ________________ 0.606 ____ _____________________
- 10) LU t0.35 21.44 3'.01* U,)O9 7; 10 10 15.66 5.57 7 0.595 16.6 95.0* 0.566 21.3 -2.2 1410%ILntake 0.737 0.461 MSD = Minimum Sirtlni a-t Difference Dunoeff.'s M5D vanne: 0.0721 PM48D- Ferrcri Mi~nium Signifler.ot Difference 03.0 percent differenoe betweenthe eoab'ol wtdtirealmoent P1450 isan mennoue of test precision. The 1'MSIDis the mninismum significant mnawhole effluent toxicity lest that car be declered atatlstica11y Dunneti's 6660 value: 0.1286 Lower £1451 hatred determoined by USEPA (10th percentile) = 12%.
£6M80: 23.3 Upper PMSD bossnd determined by US8PA (90th perrerstile) = 10%.
Lower and. upper PMS50 b*oundswere determined from the lOtliand 901kperetle, respectivey, of P1430 data hoot EPAb WEI"I-ttulehoratosy Voribility Study (USSPA, 2001a; USE.PA, 200thb).
USEPA. 2001o, 2001b. Final Report: lnterlaborutosy Variability Stadyof EPA Solmrt-trm Chroaloond Ac~ute Whole Effluent Toxicity Teat Methods. Volumes 1 cod6 2-Appendix. EFA-821-B-01-004 and EPA-821-B~0-00.(X5. US Environmental Prcieclion A~eec, Cincinnat, OH.
Flu: .sqnl 01_i02Ol4Sdata.sxta Entered br: .1.S*.no Reviewed by:.!
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015.
a statistical Analyses
~ EnvJronm~ntaITe~t1nfl So1ulIor~.
Inc.
Larval Fish Growth and Survival Test-7 Day Survival Start Date: 1012012015 Test ID: PpFRCR Sample ID: TIVA /SQN Outfall1101 End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sol.- Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2015 Protocol: FVWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000.
- 10.7 1.0000 1.0000 1.0000 1.0000
- 21.4 1.0000 1.0000 1.0000 1.0000 42.8 1.0000 0.9000 1.0000 1.0000 85.6 1.0000 1.0000 1.0000 1.0000
- 100 0.9000 0.6000 0,6000 0.9000 Intake 1.0000 1.0000 1.0000 0.8000 Transform: Arcein Square Root .Rank 1.Tailed Conc-% Mean N-Mean Mean -Min Max , CV% N Sum Critical D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 10.7 1.0000 1.0000 1.4120 1.4120 1..4120 0.000 4 18.00 10.00 21.4 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 *4 18.00 10.00 42.8 0.9750 0.9750 1.3713 1.2490 1.4-120 5.942 4 16.00 10.00 85.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 "4 18.00 10.00
- 100 0.7500 0.7500 1.0676 0.6861 1,2490 19.630 4 10.00 10.00 Intake 0.9500 0.9500 1.3358 1.1071 1.4120 11.411 4 Auxiliary Tests Statistic Critical . Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.73225 0.884 -0.1438 2.5003 Equality of variance cannot be confirmed Hypothesis Test (1-,tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 85.6 100 92.5203 1.16822 Treatments vs D-Control Ds-epne DoseRespnsePlot 0.9 0.8 0.7
"*0.6 W 0.5 i* 0.4 0.3 0.2 0.1 File: sqnl OI_1020l5data.xlex Bdtered by: I. Sumner Reviewed by:_ _
33 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101
.Non-treated 0
October 20-27, 2015 0
- Statistical Analyses*
© Larval FishI Growth arid Survival Test-7 Day' Growth Start Date: 1012012015 Test ID: PpFRCR Sample ID: TVA/SQN Outfall 101 End Date: 10/27i2015S Lab ID: ETS-Envlr. Testing Sol. Sample ~Type: DMR-Dlscharge Monitoring Report Sample Date: October 2015 Protocol: FWCHR-EPA-821-R-02-013 Test Specles: PP-Pimephiales promeles Comments: Non-treated Conc-% 1 2 3 4 0-Control 0.6160 0.4920 0.6280 0.5790 10.7 0.5970 0.6120 0.6320 0.5510 21,4 0.6180 0.6380 0.6590 0.6440 42.8 0.6500 0.6090 0.6330 0.5470 86.6 0.6170 0.6240 0.5880 0.6330 100 0.6930 0.3180 0.4300 0.6080 Intake 0.5090 0.5570 0.7370 0.4610 Transform: Untransfo~rmed 1-Tailed Isotonic Cone-% rMean N-Mean Mean Mmn Max CV% N t-Stat Critical MSD Mean N-Mean D-Control. 0.5538 1.0000 0.5538 0.4920 0.6160' 9.885 4 0.5939 1.0000 10.7 0.5980 1.0799 0.6980 0.6510 0.6320 5.762 4 -1.446 2.360 0.0721 0.5939 1.0000 21.4 "0.5898 1.0850 0.5696 0.5380 0.6440 8.401 4 -1.178 2.360 0.0721 0.6939 1.0000 42.8 0.6123 1.1058 0.6123 0.6470 0.6600 7.878 4 -1.914 2.360 0.0721 0.6939 1.0000 856. 0.6155 1.1115" 0.8155 0.5860 0.6330 3.163 4 -2.020 2.360 0.0721 0.5939 1.0000 100 0.5118 0.9242 0.6115 0.3180 0.6930 33.077 4 0.5118 0.8617 Intake 0.5660 1.0221 0,5880 0.4810 0.7370 21.298 4 Auxlllary Teats Statistic
- critical Skew Kurt Shapiro-Wilk's Test Indicates normal distributIon (p > 0.01) 0.95853 0..868 -0.2242 -0.9437 Bartlett's Test indicates enua.l variances (p 0.58) 2.87245 13.2767 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChY TU MSDu MSDp 'MSB MSE F-Prob df Dunnett'sTest 85.6 >85.6 1.16822 0.07214 0.13027 0.00246.0.00187 0.31073 4,15 Treatments vs D-Control Linear Interpolation (200 Resamuptes)
Point %I SD 95% CL(ExoI Skew IC05 96.016 I101
>100 1.0:
IC20 >100 0.9, IC25 >100
>1*00 0.8, lC50_ >100 0,7 0.68 0.-
0.-
0.2 0.1:
0.8
-0.1
-0.2 0, 50 100 150 Dose %
Dose-Response Plot
- 0.51 0.4i 03.3 0.2 0.1 File: sqn]Ol_102015data.xlhx Enlered by: 1.Silt~er Page 34 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated October 20-27, 201.5 0
Statistical Analyses EtwlronmentalTestlng Solutloni. Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10/20/2015 Test ID: PpFRCR Sample ID: "rVA / SQN Outfall 101 - Intake End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Pate: October 2015 Protocol: FW'CHR-EPA-.821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 .3. 4 D-Control 0.6160 0.4920 0.5280 0.5790 10.7 0.5970 0.6120 0.6320 0.5510 21.4 0.6180 0.5380 0.5590 0.6440 42.8 0.6500 0.6090 0.6330 0.5470 85.6 0.6170 0.6240 0.5880 0.6330 100 0.6930* 0.3180 0.4300 0.6060 .
Intake 0.5090 0.5570 0.7370 0.4610 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Mini Max CV% N t -Stat Critical MSD
- D-ControT 0.5538 1.0000 0.6538 0.4920 0.6160 9.885 4 1.0.7 0.5980 1.0799 0.5980 0.5510 0.6320- 6.762 4 21.4 0.5898 1.0650 0.5898 0.5380 0.6440 . 8.401 4 42.8 0.6123 1.1056 0.6123. 0.5470 0.6600 7.878 .4 85.6 0.6165 1.1115 0.6155 0.5880 0.6330 3.163 4 100 0.5118 0.9242 0.5118 0.3180 0,6930 33.077 4 Intake 0.5660 1.0221 0.5660 0.4610 0.7370 21.298. 4 -0.185 1.943 0.1285 Auxiliary Tests . Statistic Critical' Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) "0.93339 0.749 1.07115 1.28224 F-Test indicates equal variances (p = 0.23) 4.85006 47.4683
.Hypothesis Test (1-tail, 0.051 M$Du MSDp MSB MASE F-Prob df Homoscedastic t Test indicates no significant differences 0.12863 0.23229 0.0003 0.00876 0.85928 1, 6 Treatments vs D-Control Dose-Response Plot 0.7' 0.6.
1-tail, 0.05 level
(- 0.4. of significance
- 0.31 h,~p*~.ot R.~d.Wby Y.eij.iLJC..r.n:
File: sqnlOIWl2OlSdata.xlsx Entered by: .1.Sumner Reviewed by:
Page 35 of 98
'* ........ Page 5 of 6 Species: Pimephalespromelas Date: 10-20-15 Client: TVA'/Sepuovah Nuclear Plant. Outfall 101. NON-treated Daily Chemistry:*
- _Day Analyst
=*====TConcentration Parameter /
pH (S.U.) i .....
DO fmn/I' A. -,
Conductivity CONTROL (~unhosIcm) 301 NON-treated *Alkallnity
- (mg CaCO3/L) ________
- Hardness (mg CaCO3IL) _______
"1Temnerature (CC) .~ .- ~
i pH (s.u.) S~O2~ ~ -j 10.7%
DO (ma/LI Conductivity * .
L1~ .1
- ac~~
- Temrjerature (CC')
a - -
pH (s.u.) *,3II 77 17 ,.q
- U7.$# It DO(mu/L) - 7 21.4% Conductivity II
(~+/-mhos/em) 2YA
~ratur~0 c~ -iA.
S 42.8%
pH(S.U.)
DO(mafL')
Conductivity I ~.Q2- b~t 0a T1L (Mrnhos/cnl)
- Temnperature (°C)
S I. ~
nH (S.Ul
- ,.o'2.
-- 1:*
DO (mrJL) 85.6% ' Conductivity
- Temperature (°C)
- o~& -~-
Conductivity ~ <.0
(~cmhos/em) 100% (mc CaC0~/L) a
- Hardness (mc CaCO~dU
- TR chlorine (rng/L) do
- Temperature (°C) k~l a - -
oH (S.U.')
...... *- I " "-- 41,,
I ~A 7q~ H DO (ma./L) .0 lb I U Conductivity (unrhos/em) ~I-v4~, 11 10o% *Alkalinity
- ntake (nig CaCO3IL)
- Hnrdaess (rag CaC0~IL)
- TR chlorine (mag/i) 0.10 a * ,' C.
11ita Fna niia inl Initial Fia
.dpn*. peratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
/ ,kanity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for e~ach analysis and transcribed to this bench sheet.
(*":
8**.Total residual chlorine was performed on non-treate 0utih 101 anid Intake samples.
\ [A / **___" "SOP AT20 - Exhibit AT20.3, revision 11-01-14
. Page 36 of 98 """
........ Page 6 of 6 Species: Pime~halespromelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. NON-treated Date: 10-20-15 N
Ia a
II Day (Analyst identified for each day, performed pH, D.0. and conductivit measurements onl . '
Analyst F= I Concen-tration IParameter pH (S.U.)
DO (mg/L,) E.
Conductivity CONTROL (jxmhoslcm)
- NON- *Mkalinlty treated (mg CaCO3/L)
- Hardness (rag CaC0sJL)
- Temnnerature (0C) 'Lie.
PH (S.U.)
- 1o-~
DO (rg/L). 7 10.7% Conductivity
- Temuerature (CC) pH (s.u.)
0o (mg/L) 21.4.% Conductivity (umhos/cm)
- Temperature (°C)
-~ (OTT~.
DO (ma./LI 42.8%
I(unihosloml
- Tjnerture (00) niH (S.U.')
- DO (mg/LI 85.6% *Conductivity J '..
- Temperature (00)
DH (S.U.)
DO (mn/LI Cond~uctivity
- Alkalinity 1 100%
- Hardness I
- TR chlorine (rag/I.)
- Temneratnre (00) i'~4QQ II H(S.U. .
DO(me/L) ~7'P Conductivity
- Alkalinity 100%
Intake
- Hardness I(rag CaCOi/L)
- TR c~hlorine (mglL) 'C.
SH ~ ~- I
.............................- Initial II Final l Initial [ Final *'Initial Final Initial II Final "I..
ndp*Tperatures performed at the time of test initiation, renewal or termination by the-analyst-idlentfied in te Daily Renewal Inflo-r~mation table lo-cate d on P~age-i
[ hardness and total residual chlorine performned by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet ar:*~nty.
bky' * * - Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.*"
o*SOP
- AT20 - Exhibit AT20.3, revision 11-01-14
TVA / Sequoyah Nuclear Plant, Outfail 101 - Non-treated October 20-27, 2015 IEF Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemicai Analyses
.O Environmental Testing Solutions, Inc. Project number: 10958 Parameter Lz!L~ DaZ Da3 Da4 DeS Da6 Concentration lInidalIFinall I Initial Final Initial Final Initial Final Initial Filial Initial Final
.9.----- 802 7.66 8:13 792 8.08 7.76 pH (SU) I 5.001I '6 7.95 7.90 7.96 7.75 DO tmnn/L* 6 7.5 7.8 7.7 7.6 7.9 7.0 7.7 7.8 7.6 7.8 Codetiiy (wnhoslcmi) ___ 310 325 . 302 317 335 Control 63 64.
Alkalinity (mg/L CaCO 3) __
Hardness (mgF CaCO) L____ 88 88 -
C I 24.81 24.6 24.81 24, 3 24.8 24.6 24.7 24.4 24.9 24.8 24.8 24.4 24.9 8.02 24.6 7.77 H(SU) 802 794 802 776 797 785 799 768 800 7.61 814 790 Temperature 10.7% DOlniziL) (wnhoslcrn) 18 78 78 76 75 78 78 73 80 6.5 78 79 7.8 7.8 Conductivity _______ _______ _______ 310 2C.1 . 2'9 305 0 244 249 24.6 249 246 25.0 24.4
__________ Tcmnperature( C) 248 244 249 246 249 246 247 H(5U) 802 794 803 775 798 788 800 769 801 7.58 815 789 8.04 7.80 21.4% DO(nWL) 78 78 78 76 75 79 78 72 80 6.2 . 77 79 7.8 7.9 Conductivity (pinkos/cm) ______ _______ ______
__________ TemperatureCC) 248 245 249 246 249 24.2 247 245 250 24.6 249 246 25.0 24.4 pH (S!,J 5.021 7.91 8.021 7.721 7.981 7.588 8.01 7.711 8.011I 7.55 8.131I 7.921 8.041 7.77
- __ 7.D.9 77O_ _78m_
- 3 .8 .9 Lonnucnvnv mumao~eim, I 2:'1 I * "7J*3 7_hD '199n5es r - I_____ ~ _____-~ ~ - .:4 5~ -
I-rgemperatura ("C)
I -
24.81
-I -
.* 24.4* -
- 24.91
-0.
24.51 4
25.0[
- -~ -
S 25.0 I 24.6 24.91 24.51 25.01 24.4 nfl (SiU) 8.021
- 7.92 "8.031 %7.41 795S 7.70J 8~___ &os]__7s2J__To~j 77i 85.6% DO mnl)I
.......... I 78 .1 79 .4 j 7~_ -8.01 60 7.81 7.8l 7_Q... ]
70 r[*nndulotifvitv Ifinhnwfrlmt I -
Temperature ("C)
I-I pH (SU)_
DlO (mriL)
-a-Conductivity Isumhos/cmu) 100%
Hardness (mglL CaCO 3 )
ITotal Residual Chlorine (mn/L) I
]Temperature ("C)
I - --
IpH(SU)
DO f mo_/Ll ConductIvity (,unhos/cni) I 100%hIna !Alkalinity(ngfL CaCO 3)
Hardness (melL CaCO1 )
M i
Temperature ("C')
File: sqnl01_j02015chem.xisx Entered by: J. Surmner Reviewed by:
Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
, Species: Ceriodanhniadubia Client: Tennessee Valley Authority County: Hamilton Facility: Seanovah Nuclear Plant Outfall: 10.1 NPDES #: TN0026450 Project#: t O'V51 Dilutionpreparationinformation: Comm'ents:
Effluent volume (mL) 267..5 535 1070 2140 2500 Test organism source information: Test in~formation:
oOranismi : < 24-hours old Randomizinjg template color:
- 8.
Date and times organisms were born ID*Ol 0*A 'V* * } inculbaor number and shelf 2
- between: loaton Culture board: _____________"__lotin:
Replicatenumber: 1 2* 3 ]4 5 6' 7 sa 9 150....
Culturebhoardcup number, *, O ttIt, %.3I ~lt4 Ltlk*1 YWT bath. *0O.OtS Transfer vesselinfrmation:
lm ni) ]p= H
.02 Lot S.U. Ternperature = 1L..tvrg LSelenost Cse rum batch: Oi'2 AIS Daily renewal information:
Day . Date .Test initiation and feeding, MHSW Sample numbers used. -Analyst renewal and feeding, or batch used Outfall 101 Intake
- -termaination time ,
0 10-20-15 t* '*t-S- 6 i% ti,(3\ *._______*____-
- 2 io~-is~ ~i~-z~r' ~sc~-A~l 6 10-26-15 60Oo I~rb iS1 ioU. 05.
,7 10-27-15 .* t Controlinformatiorn Summary of test endpoints:
_______________________ Control-1 Control-2 Acceptance criteria ______________
% of *ale Adults: . C)?.07 <20% 7-day LCso __________,
'%Adults having 3rd Broods: !1 1.' 1Ct'7o >80% NOEC .l1001.
% Mortality: . .. Q [o. ______
- 2o% LOEC _________
Mean Offspring emal'e: ______o
- o*. 15.0Ooffspringlfemale ChIV *..100
_________o3 7, < 40.0 % ie ,
Ad.pend.n, R.~4.wb~
SOP ATI 1 - Exhiibit ATl 1.2, revision,11-01-14 Page 39 of 98
@* * .*T. *Page 2 of 7 Species: Cer'lodaphniadubia Client: TVA I Sequovah Nuclear Plant. Outfall1101 Date: 10-20-15 CONTROL-.1 ,Sun'* ival andRepJroductionData Day Yongrdu 1 1
- 2 3 4 Replicate .number_
5f 6 7 f819 10 Y
Adult mortality ioungprod.ced
__TAdult mortalityJ~ ,j..
k
- 1 o,~
I
.. Lk..
.odo
\
.- _L.
\ *
- o. 1o
- I 0 -
.o,. o.o.IL I
-x
'- I '-i k
... 3iYoung produeed O, 0' LQJO 010* 0 010* 0[*.I
[4IYoungproduced A 4*iiLA..- I___
6
__fAdul 1
tmortality You.pr.u.e Young produced j]Jp
- I '.-'L ...
'IL.1~7F V iof' o
r~.I~1V.I~
c: 0 i'.
___ Aduit mortality I ' i *l.. * '-I '-* I_'l-.l '. - I I'** I*
To]a youngproduced. .*"- '-. vl 1 iW l I SX X v.'*-
Fhnal Adult Mortality X for3*Broods
'*'....{
.~ "
I "*, I * ,I*
Nsre*:Alt mostlisy (L fi ve,D - dead), SB - split brood (sigle brood splitbetween two days), CO - cony over Co*yrlo csic over-with adult dinng trmsn5r),
CONCe 10.7% survival and1?eproductionData
____Replicate number ___.
2 I Youngproduced *I*~I0 1... .)O _
I Young'produced' I___ o l I ___1' o 1 L
[Adultmortali - *i \.---I -I.-- x I v-- L*-'.!k-I - I- 1 -
Yonprdce i4~ .1% -... .. 2. III -I 6 Adultmnortali7" ty *J IT'. __ LJ '*. j I S'i '
Total young produced I -' * [ :*
- j *' L * *I
- I Final AdultMortalit, .... I,-= .. -* * .: -.. -. ,.-
TV .liJX sonsgnsa assseusavervein sesasuwIerlaaaaseTI.
Con canfradon.
Mean Offspning/Feniele: I ~%~S
%Reduction from Control-I:.
ReSseby sailsyL Knwm.aI LA..- Page4Oof98 SOP ATI I - Exhibit ATI i*.2, revision 11-01-14
'*'*F.*....*i~st~lsollon~a¢,Page 3 of 7 Species: Cefiodapliniadubia Client: TVA / Seauoyah Nuclear Plant. Outfall1101 Date: 10-20-15 CONC,. 21.4% Survival and Reproduction Data I - . Replicate number SYoung produced O Q) c*) j* OcL* 101
- Adult mortality 2Yun.podce.... c 1 oA0101 Ir o-1.
- k. .- *- ~-* . ___ * - .
_ _ Adult mortality j . 1-1' [
- 1
'* t -- *-I 3 Young produced 100 0 .. Jy..I0[DI,.. ' '0 4 Young produced.Z 1~J.51.5. 'Jjj
__nal AdultiMortality I_-_ _ *. *s ____ .- . Ls - '-.
___ AdlMortait s-___ lity- __ __
6 Yunprdued~le J0 Of[fspring iIna0:I 14 ougprdce.8%
7Oe
-av Tot. [Young produced
,, 1-
- ) C I..3 -
- u+/-iv
%. and Rpou
- I tionData.
0%
9 -
4 L~oungproMean .Of,.sprIngl ema1e COC 428 SuouvanandprodducionDat 21 = - [ - - I ,..
1 Young produced 0 01m t Q, o***t*t 1'* I**
2Ttayoungproduced -I.0 *
- O &QjO1 ' 0 . 0
__na Adult mortalit *--
- L [** * '- "-- * -
P/ole:AGOIOotwtality tL live, IJ eeao~. liH = tpiIt DroooLelo8le0/000 spotocoweentWOOayO).LU = en Concenlrttlon:
% Mortality: .1.~
ffMean Ot~pring/Female: I $ *&
SOP ATI I - Exhibit ATI 1.2, revision 11-01-14 Page 41 of.98
- .,,s,. Page 4 of 7 Species: Cerlodaphniadubia' Client: TVA / Sequoyah Nuclear Plant* Outfall 1.01 Date: 10-20-15 CONC: 85.6% Survival andReproduction Data
- . _____ - Replicate aumber Da* __ _ 1 2 T314 516 7 18 9110 IFYoung produced O. (0 0100101010**)
[Aduit mortality t.... LJ , _ .-. - '.__I T[
Young produeed I O Id O OL * [I iI C 1 0 Lo. I O I 0
-IAdult mortality I - *- - I '- I t -. I
- 1 -I *-11 *-f I
_[ Adult mortality.I L- --. *- 1I* A[1 4 Young produced ~ __ -- ~__
SYoung produccd
___[',.'Adult mortality.*- IJ TL *I*
~L_-i1%--
- I*II~b J I.*
[.I__
'- I_ "-J-.- '~
6 Youngproduced [ * %* J '.C)" I I..t t l *1 I'P1' Total youngwpoduc~ed "*' .'* *-t *' I -"* " "* 1 * .*
F~lnal Adult Mortality -c: *- '- '- f %-, . s.*%-- j, *- __
lease: AOWSmommy IL ov~ I?= 0050). hki spill OWOO~eiogIe esosoaspin oesweon two 00)5), Lv CWOvover(o5asmemm v~aiilil00tOU0hilRO~~~
I%
3 Concentraton.
Mortality .. 0?".
MeanOffspring/Female:. I o*,
% Reduction front Control-i1: f-l *o CONc: 100% Survival andReproduction Data 1 ____....Replicate number_ _ _
Day Ii Young produced 1112 (0 J 0 3
0-4
- ... 10 iS
.x* 617
.n" ... -O 819 J....... 1
.110
___ Adult mortality 4 t . L_ __ L_.. "- 'I_ .-i
- __.___
J Youngprodced IAdult mortallty __
Oj Cb0 j
I . --
0Ig
-.. I L I*
'L-I.--_ 1 I*
-I..--
- oung produced Y
Autmortality tI t4_-_
0 ~
I
- t't
~~~x.
l) -d t* I-* I.
' ' I~
'JAdult mortality J '% , -I '--IkJ- I'- I '-1 V-I ',,1 __
Young produced 0 " ~~ f0 "~- I(1
'W "
FiIa Adult m ortality I
- _'- '-1, '_, 1 ' -. L. 1., .. j I.. '
Naote:Adult mortoilly (L = live, D - deed), SB - spilt brood (single brood split between two doys), CO= a iv over (aofsfbolo caroind over with adult during tran~lr).
Cotice, traikrn-S% Mortality: 07 SOP AT11I - Exhibit AT1 1.2, revision 11-01-14 Page 42 of 98
- L* n~mmenal~e1*rS~mlm.Page 5.of 7 Species: Ceriodaphuladnbki Client: TVrA / Sequoyah Nuclear Plant. Outfa~l1.101 Date: 10-20.-15.
CONTROL-2 *Survival and Reproduction Data
........ .... : ....... ... Replicate num ber____
Day ____ 112 3 4 516 7 8 91i0 Young produced .0 1 0 O,...
0 - 0 0 O
- O
__[ Adult mortality %] *- '-- *. j% ~ \._
- k-- '
JI +I Lo.. Il...... 2I .. i ... L -
.2
_I_ Young produced Adult mortality. ____ _--_
. [ )"
-I -I c .. - I *
"C*...
- IJ --.-
4 I Young produced I [AJ'A '14 0IO I ~II OW '4fO J. Adult mortality J k..l.ŽI--_-I '-[ 'I- I*- I'- '-"
6 Aduitmortality, Young produced ,
1* ,
Q"L
___T.
Q [
J L.oj*_ L
, .*.,I c"1,*
" ' Young produced * , " t.*
%l,*,,* * , ,'4 %l .t, Total young produced '*n* '**
Final A~dult Mortality %- \.. %--- %- *- a-. *- ** L-..
X for 3'aBroods .'~- E** 2 * *. .T -
A.ls/a Adul esunls *.'ive, D =dud), SnD apli brood (snl~e brood split betwama two days). CO: carry over (offspring carried ovu"vith adult during trasrer).
SConceniradon:
+ % Mortality:
- 07'F" CONC:: 100% Intake Survival and Reproduction Data 1.) ____Replicate number Day ____...._ 1 2 1 -3 1 4 1 5 1 6 7 1 8 9 10 IAdult mortality j *_. '-. i' _. __ '-*
__ Adult mortality * *-- JI JI *- _ *- _ *-
Yon mot]ty prdu ced
.I 'I,.-"IQ.. I"I0... ..*LL .... *O..I L...
u,.-,+~ I: . *....0 [ C2.. ..
p5-' L..C..
I t..:. ,,,,,,.+ I ...Xy....Lo.- .. 0 I o.- X...~..J...:..
3 Younporduce YonpodetAdultmortlily I " -I'.-J I ,-.it+I,'-- '+ I___ '- I,+.`
64 *Aduttmortality Young produced '-' H-iI'*-,~Ia4 IIl '*..I]'I
' ' '- '-SI'4I I *- - ll' Youpoue f' .1
- I *I+'4 -.
Tota young prodclued j+ 0 I .+1 q J L.
- "<"" .. (i
%+.. 1 C L..
Final Adult Mortality L* .L- * *. L.. %- L.. t. L.." .
Note: Adult rrortnliiy (L - lIve D 1 dead), SBt splitlirood (SaingiIroodlspllt between two days).CO= cawver toffsprsui ~~taed over withadult Onhgmsssitran.)
Mean Offspring/Female: . * '
5s4ve.nd.es posies,by % Reduction from Control-2: ,=o' ShinyLilnenpe SOP AT11I - Exhibit ATI 1.2, revision 11-01-14 Page 43 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated October 20-27, 2015
~*HI. S Verification of Ceriodaphniaz Reproduction Totals (a B ID Ezwh.oemenaI Inc.
TesOftgSoIul1oo~.
Control-I 85.6%
ii 1 R.~ Ikate number 4 isl 6 FTF~
Total Day 1 2n-3-r4 .5 6 8 9 10 J ID Day 1 ~ 2 1 3 o jo ~o 10 j0 J 0 0
0 2 1 .0 010 0 0 0
0 010 0 0 O,
0 0 I 0 0 0 0
0 0
0 0
OH to K
0 7 1:1 . - -. - -. -
3 0 0 0 0 0 0 0 .0 0. 3 0 00 (I 010 0 0 0 0 0 0i F' 4 5
6 Total 10.7%"
0 5
11 4
11 0
1414 13 1616 301 29 4
12 0.0 29 3
11 30 4
10 0
30 4
I3 0
1 531339J 2
O 0J0120 4115 .141171151 12 31 J31J31j32j28j301J 1
[0 1
1481 5
6 7
- Total 12 1413 0
4 17 33 38 0
6 18 0
6 15 18 37 32 13 1213 0
4 .5180 35 37 0
5 19 1I1111212 0
5 17 33 0
4 18 33 0
5 16 33 0
4 20
- 36 "123 0
48, 176 34 Replicate numberToa DY *1 2 3 4 5 6 7 8 9 10 Toa 1 0 0 .0 0 0 0 0 0 0 0 0 2 0 0 0" 0 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0
- 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0. 0 4i 4 5 5 4 4 5 3 4 5 4 43 5 14 12 14 13 12 14 12 12 14 12 129 6 0i 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 15 14 15 13 16 15 16 17 16 154 7 17 17 19 20 17 20 18 19 17 18 182 Total 33 31 30 .30 30 32, 30, 32 .35 32 315 Total 36 34 39 38 32 39 35 36 37 36 362 3 .i0 0 0 0 0 0 0 0 0 0 21.4% _______ Control-2
- Replicate numberTol Day 1 2 3 4 5. 6 7 8 9 10 Tol 1 0 0 0 0) 0 0 0 000o o 1 0 0 .0, 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 .0 0 0 0 2 0 0 0 0 0 0.0 0 0 0 0.
3 0 0 0 0 0 0 0 0 0 .0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 5 4 4 5 4* 3 4 43 4 4 4 4 3 5 4 4 5 4 4 41 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 16 15 18 14 16 17 17 16 161 7 15 14 14 16 12 15 14 16 16 15 147 Total 33 3234 31 3531-3334 3232 327 T..'ota 30 28 30 29 29 32 29 33 32 30 302 42.8% ___________ _______ 100% Intake__________
Day 1 2 3 4 5 6 7 8 9 10 Toa 2I 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 .0
__ 0 0 0 0 0 0 0 0 0 45 0
6 0O 0 0 0 O 0 0 0 0 0 0 156 Total 32 36 32 34 34 33 33 34 31 33 332 Total 332
.2i1.~. J33 *~ *~* 31j32 File: sqn1 01_10201 5data~xlsx Entered by: J. Scquirer Reviewed by:.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated "0
October 20-27, 2015 Ceriodaphniadabia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 0 . Quality Control Verification of Data Entry, Calculations, and Statistical Analyses W" Environmental Testing Solutions, Inc.
Project number: 10958 Concentration _________ Replicate number Survival Average reproduction Coeffceint of Portent redoct'on from
(%) 1 2 3 - 4 5 6 7 B 9 10 (%) (offsprlng/female) v~~o ('A o~trl (%t)
- Control -1 30 29 29 30 30 31 31 31] 32 28 100 30.1 4.0 Not applicahle 10.7% 33 31 30 30 30 32 30 32 35 32 I00 31.5 5.2 -4.7 21.4% 33 32 34 31 35 31 33 34 32 32 100 32.7 4.1 -8.6 42.8% 32 36 32 34 34 " 33 33 34 31 33 1100 33.2 4.2 -1[0.3
- 85.6% 33" 38 37 32 35 37 33 33 33 36 1100 34.7 6.2 -15.3 1010% 36 34 39 38 32 39 35 36 37 36 100 .36.2 6.1 -20.3 Control -2 30 28 30 29 29 32 29 33 32 30 100 30.2 5.4 Not atpplicable 100% Intake 35 34 34 33
- 36 34 31 .- 32 31 32 100 33.2 5.1 -9.9 Outfali1101: MSD = Minimum Significant Difference DunneUt's MSD value: 1.743 PMSD = Percent Minimum Significant Difference PMSD" 5.8 PMSD is a measure of teat preoision. The PMSD is the minimum percent difference between the control and treatmnent that can be declared statistically significant in a whole effluent toxicity test.
Dunneft's MSD value: 1,282 Lower PMSD bound determined by USEPA (I 0 th percentile) = 13%.
PMSD: 4.2 Upper PMSD bound determined by USElPA (9 0 ehpercentile) =47%.
Lower and upper PlvSD bounds were determined from the 10th and 90th percentile, respectively. ofPMSD data fr'om EPA's WET Interlaborutory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Pr~otectioni Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratoty Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0I-004 and EPA-821-B-0l-005.
US Environmental Protection Agency, Cincinnati, OH.
File: aqnl 01 10201 5data.xlsx Table populated from associated "Verification.of Ceilodaphnla Reproduction Totals" spredadheet.
Spreadsheet entered by: J. Sujnner.
S Reviewed by."
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated October 20-27, 2015
- * * *: Statistical A.nalyses Cenodaphnia Survival and Reproduction Test-Reproduction.
,Start Date: 1012012015 Test ID: CdPRCR Sample ID: T[VA/ SQN Outf'all1101 End Date: 10/2712015 tab ID:. ETS-Envir. Testing Sol. Sample Type: DMR-Disoharge Monitoring R~eport Sample Date: October 2015 Protocol: FWCHR-EPA-B21-R-02-01 3 Test Species: CD-Cerlodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 Control-I 30.000 29.000 29.000 30.000 30.000 31.000 31,.000 31.000 32,000 28.000 Control-2 30.000 28.000 30.000 29.000 29.000 32.000 29.000 33.000 32.000 30.000 10.7 33.000 31,000 30.000. 30.000 30.000 32.000 30.000 32.000 35.000 32.000 21.4 33.000 32.000 34.000 31.000 38.000 31.000 33.000 34.000 32.000 32.000 42.8 32.000 38,000 32.000 34.000 34.000 33.000 33.000 34.000 31,.000 33.000 85.6 33.000 38.000 37.000 32.'000 35.000 37.000 33.000 33.000 33.000 36.000 100 36.000 34.000 39.000 38.000 32.000 39.000 35.000 36.000 37.000 35.000 Intake 35.000 34.000 04.000 33.000 36.000 34.000 31.000 32.000 31.000 32.000 Transform: Untransformed 1-Tailed Isotoni.c Conc.% Mean N-Mean Mean Min Max CV%/ N t-Stat Critical MSD Mean N-Mean Control-I 30.100 0.0967 30.100 28.000 32.000 3.977 10 . 33.067 1.0000 Oontrol-2 30.200 1.0000. 30.200 28.000 33.000 5,362 10 10.7 31.500 1.0430 31.600 30,000 35.000 5.238 10 -1.837 2.287 1.743 33.067 1.0000 21.4 32,700 1.0828 32.700 31.000 35.000 4.090 10 -3.412 2.287 1.743 33.067 1.0000 42.8 33.200 1.0993 33.200 31.000 38.000 4.212 10 -4.066 2.287 1.743 33.067 1.0000 85.6 34.700 1.1490 34.700 32.000 38.000 6,233 10 -6.036 2.287 1.743 33.067 1.0000 100 3,6.200 1.1987 36.200 32.000 39.000 6.080 10 -8.005 2.287 1.743 33.067 1.0000]
Intake 33.200 1.0993 33.200 31.000 36.000 5.080 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.65447 1.035 0.13015 -0.266 Bartlett's Test indicates equal variances (p = 0.34) 5.7073 15.0863 The corntrol means are not significantly different Ip = 0.88) 0.1 5703. 2.10092
,Hyrpothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.74259 0.08789 47.7867 2.9037 7.7E-10 5, 54 Treatments vs Contrel-1 Linear Interpolation (200 Resamples)"
Point % 50 95% CL Skew 1005 >100 1010 >100 1C15 >100 1.0 IC20 >100 0.8 IC25 >100 0.8 I040 >100. 0.7 IC50 >100 0.6 0.5 S034 S0.2 0.1 0.0
-0.1 '. . . . = .
-0.2
-030 50 100 150 Dose-Response Plot 45 405 30 1-tail, 0.05 level
- =25 of significance
-20
- 10
.1 a a; a o* =
- p *File: sqnlOl102O15dat~ylsx ihse by .U8 Page 46 of 98 **~*_*
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated October 20-27, 2015 0
0 Statistical Analyses En~IronmeflthIte tlnuS~hdi~n; Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 1012012015 Test ID: CdFRCR Sample ID: TVA I SON Outfall 101 - Intake End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sal. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 201,5 Protocol: FWCHR-EPA-821-R-02-01 3 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Cone-% 1. 2 3 4 5 6 7 8 9 10 Control-i 30.000 29.000 29.000 30.000 30.000 31.000 31.0.00 31.000 32.000 28.009 Control-2 30.000 28.000 30,000 29.000 29.000 32,000 29,000 33.000 32.000 30.000
- 10.7 33.000 31.000 30.000 30.000 30.000 32.000 30.000 32.000 35.000 32.000 21.4 33.000 32.000 34.000 31.000 35.000 31.000 33.000 34,000 32.000 32.000
- 42.8 32.000 36.000 32.000 34.000 34,000 33.000 33.000 34.000 31.000 33.000 85.6 33.000 38.000 37.000 32.000 35.000 37.000 33.000 33.(000 33.000 36.000 100 36.000 34.000 39.000 38.000 32.000. 39.000 35.000 36.000 37.000 36.000 Intake 35.000 34.000 34.000 *33.000 36.000 34.000 31.000 32.000 31.000 *32.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max -CV% N t-Stat Critical MSD Control-I 30.100 0,9967 30.100 ,28.000 32.000 3.977 10
- .Control-2 30.200 1.0000 30.200 28.000 33.000 5.362 10 *
.10.7 31.500 1.0430 31.500 30.000 35.000 5.238 10 .
21.4 32.700. 1.0828 32.700 31.000 35.000 4.090 10 42.8 .33.200 1.0993 33.200 31,.000 36.000 4.212 10 85.6 34.700 1.1490 34.700 32.000 38.000 6.233 10 100 36.200 1.1987 36.200 32.000 39.000 6.080 10 Intake 33.200 1.0993 33.200 31 .000 36.000 5.080 10 -4.058 1.734 1.282 Auxiliary Tests . .Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92371 0.668 0.31324 -0.9934 F-Test indicates equal variances Cp= 0.91 ) 1.08475 6.54109 The control means are not si nificantiy different (p= 0.88) 0.15703 2.10092 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedestlo t Test indicates no significant differences 1.28211 0.04245 45 2.73333 7.4E-04 1, 18 Treatments vs Control-2
"' Dose-Response Plot 45 40 35 303 -
- 25 TI I I I-I-------------------------------
I 1-tail, 0.05 level of significance 0O I- 2 0.
I- ~ CD CO 00 rj, o c~~j IL)
- e.j 'e' CD
- Page 47 of 98 File,: sqnl 0lO12015data.xlsx Entered by: 3. Sumner Reviewed by:
Page 6 of 7 Species: Ceriodaphniadubia Date: 10-20-15 Client: TVA / Sequovah Nuclear Plant. Outfall 101 Daity Chemistry:
I (Analvst identified for each day. nerfhrmed DayD.O_
M oH.
and 'ondiic~tiuit.- .~apo.IwemPn'o
--- - - .--- .-. ~
rnnlv.*
-. Ii---
0 I 1 i Analyst '
Concentration ] Parameter DO (mn/LI itdiictivity (ra*L*
(tunhos/cml
- DO CONTROL *Alkallnity (mg CaCO i/LI
- Eardness (mCaCOi/L)-
- Temperature (OQ2 4 - - U -- S -
oH (S.U.)
DO (snaL) 7.~1 II *.* I *, -'. ':
10.7% Conductivity
- Temperature (00) "*AA 1~
1l~i -~
tq.
~
I
- *1 .
~!i(~LI ~¶2 ____ 7.97 2 1.4% Conductivity I ~ -
0 1
- Temperature ( C) l'~ b.
DOm
____ LZI~
it i.'iq 1 DO (mal/L) II 42.8%
Conductivity
- Temneratuiret°C)
J~LS I.- 4~
pH (SU.)
DQ~g/L) -74 85.6% Conductivity 1 ~
- *Temperature(OC- - A..% U IS -fl.o L. I _ .*O Ii:**
Conductivity 100%Alkailinity
- Hardness ,*. .
- TR chlorine (mg/L) **' t10
__________ *Te )erature(C _. 1. 0 -.
- Conductivity i - -
100%
- Intake *Alkcalinity (mgCaC 3IL
=,.*
=== ==* *TR chlorine
- (mgfL). C 0.oIQ -* -
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual ojlorine performed by the analyst identified on the bench sheet specific far each analysis and transcribed to this bench sheet by:" :"
SOP AT11I - Exhibit ATI 1.2, revision 11-01-14 Page 48 of 98
- * '*""'*'*Page 7 Of 7 Species: Ceriodanhniadubla Client: TVA / Sequovah Nuclear Plant. Outfall1101 Date: 10-20-15 I
Day (Analyst identified for each day. n~erormied pH. D.O. and conductivity measurements only.) Im II 3 I. 4 I .1 5 I Analyst Concen-Paaer trationt "_____" __
pH (S.U.)
DO (mg/L)
Cohnductivity
(~mos/cm)
CONTROL *AN~aljnity (nig CaCO 3 IL)
- Hardness (nig CaCOilL) rI ~
- Temnerature (0C*
-, -- .- i~i .z. - -
DH (S.U.)
DO(rng/L) 10.7% Conductivity (jimhnslcm)
- Tamnan.o+....o 1"Cfl
- a. -
s
~ -
= fl.np.A S.... - ~
pH (S.U~)
DO(mgfL) 7 21.4% Conductivity ~qLJ
__________ *Temperature (0C)
- p11 (S.U.)
- DO (mg/L) 7.
42.8% Conductivity (prnhcslcm)
___________ *Temperature (00) pH (S.U.) :
85.6% ConductIvity (unhoskm) t Temperature (0C)
- V uR (S.U.i
. ..... _
- I-Conductivity 100%
- Hardness (mig CaC03L).
- TR chlorine (ragiL)
- Temnerature (°C1 m m I
pH (S.U.)*
DO (m*./L' 100% *Alkalinity Intake SConductivity
- Hardness
- TR chlorine (mg/IL)
II SInitial I ina.I Xnitia I Final I Initia . ialIII~nilia, II inF
- Temperatlures performed at the time of test initiation, renewal or termination by the analyst identified in thle Daily Renewal ,
information table located on Page I. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench "l sheet specific br each analysis ad transcribed to this bench she-etby: ._._.'
1 t**
S6P AT11I - Exhibit AT1I.2, revision 11-01-14 Page,49 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated October 20-27, 2015 Ceriodaphrniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Enviro.nmental Test~ing SolUtions, inc. Project number:. 10958 Concentration Parameter Da0D 1D a a4Da5D 10958
______________ nta ina Inta ia nta ia nta ia nta ia. Iiil Fnl I il Final DO (mulL) 7. . . 7 7 . . 79 79 79. 77 79 76 7.9 Control Alkalinity (mg/L CaCO3 ) 6 . 6 6 Hardness (mgIL CnCO3) 88 888
_______Temperature !CC) 248 2.,49 2. 47 2. 4. 49 2. 52 2. 51 2. 25.2 Hi 5 .2 79 .2 7.2 79S.7 . 7.9 79U.0 807 81).9 80 8.01 DO (mu_.L) . . . . 78 78 7.8 .. . . . . . .
10.7% _Conductivity 0zmhoslem) 31 1 9 30.21* 9 0 *, :dl.'*
_______Temperature CC) 248 .2. 49 2. 4. 45 2. 51 . 2.0 2. 47 2. 50 25.0
- ., (sU) 8.2 79.80 .2 7.8 79.0 .8 801 80 . .9 80 8.01 DO (mulL) . 78 79 78 78 75 78 78 79.0 79 77 79.8 7.9 21"4%/ Conductivity (nznhos/cm) , _____ __________24 8027 *29
,Temnperatre °C) 48 2. 50 2. 49 2. 4. 51 2. 52 2. 49 2. 25.0 pH (S 82 79 82 79 .8 79 .0 .8 81 89 83 80 80 8.01 4.o DO (me/L) 787-9 79 76 . . . 0 0 7 0 79 7.9 4.% Conductivity (jumhosfm) _____ 2tY' 267 Temperature (°C) 24,5,5 48 2.9 2. 49 2. 50 20 27 2 50 24.9 pH(SU) 80,0,0 79 .5 7.9 80 .0 0 0,85 82 80 8.01 Ds6/
o (mnlL) 78 7 9 79 76 78 78 80 8 0 7 9 79 7.9' 8.% Conductivity (junmhos/cmn) 2,6 ____ 31 0
_______ emperature ( C) 24'51 20 2. 5. 48 2. 50 25 4 4 4 50 25.1 pH (SU) 803 .80 8.3 79 7.3 80 8.3 79 8.1 80 78 80 807 7.99 Do (mgIL) . . . . 78 78 7.8 76 80 7. 8 7 . .
Conductivity (nnlos/em) 17
- 8 7 8 8 8 8 100% Alkallnity (mglL CaCO3 ) 6 7373.
Hardness (mg(L CaCO3) 90 73 69 Total Residual Chlorine' (mulL) < .1 .<00- <01 S Ternperature 248 .2. 5. 49 2. 2. 50 2.2 2. 51 24 4 51 24.9
- H ISU.80 .0 80 .6 .8 80 .3 80 79.4 89 84 80 8.03 DO (mulL) 78 78 80 79 76 78 78 77 .. 80 78 77 79.0 8.0]
Conductivity (pmlaos/em) 17 7 7 8 8 8 9 Hardness (mgIL CaCO3 ) .6.7 . .6 Temperature (0C) 47 2. 50 2. 49 . 2. 49 2. 49 2. 49 2. 24. 2.9 File: *sqn101_.102015cliem.xisx Entered by: J. Sumner.
Reviewed by:
Page 1 of 6
"* Chronic Whole Efflueni tToxicity Test (EPA-821-R.-02-013 Method 1000.0)
- 1 .."-" . .~
pecies: Pimev/hales promelas
- lttA~itI. Jt* nlog V dIl~tY tLkUtlIUI l County:
Outfall: Ilamilton 101 Facility: Sequovah Nuclear Plant NPDES #: T1N00264.50.
Project #:
Dilutionpreparationinformation: .Comments:
Dilution prep (%6) 10.7 21.4 "42.8 j85.6 " 100' Each concentration was UV-treated Effluent vohrnie (niL) 267.5 535 1070 2140 2500 for 2 minutes to remoVe pathogenic Diluent volume.(iL.) 2232..5, 1965 1430 I360. 0 . .Interferences..]
Total volume (niL) ... 2500 2500 2500 2500 2500 "Testorganism'information: .... Test information: .... __
"Organism age: itA -* "t \*,1 %* . *L Randomizing template:' ______,_
Date and times organisms 10-19-15 1600-1700
- In.cubator number and w~ere born between: .shelf Iocation: ______
Organism source: ATOX(Batch Pp: !10-19-15 "Arterma OHM number: *C11M836 1)rying informationfor weight Transer pH= bol~determination:____
~
infrmatin:
Tranferbowlinfrmaton:pH= *.oo' ",U. Date / Time in oven: I 1,')*..4 * .
Temperature = X... * ° Initial oven temperature: J ¢_ *" t Average transfer volume: 0.1749 mL " Date/'Time Out of oven: J lo*I-t*
Final oven temperature: I a"CV(
____________ ________________Total drying time: "j -*
Dailyfeeding and renewal information:
Day Date '" Morning feeding Afternoon f'ee~ding Test initiation,. Sample numbers used MHSW renewal, or batch
_____ termination used
... Time Analyst Time. Analyst Time Analyst* Outfall 101 l Intake..
3 10-23-15 o>* ..] * *'Ac ...4...... N ..4j".. tI¶Ui2,1 t1 ~ icD2&.* 14 IO'20" S 6 10-26-15" '~frr~ A I, (04b ISIb1~ .cA tts-'l*-tS
[ 10-27-15 I~2JI~i Coqtrol information: Acceptance criteria Summary of test endpoints:
I%Mortality..
I vrg egtprinitial larvae:
Average weight per surviving larvae:,,
I 0120%
I 0 S¶ O.6g
,, Ž,0.25mg/larvae j]7-a NOEC LOEC LCs5 .
I[ til
[0 ChV "7 jtj7.
IC2 5 ~
SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 51 of 98
, ..... Page 2 of 6 Species: PimeDhale* promelas Client: TVA / Sepuo~vah Nuclear Plant. Outfall 101, UV-treatedl Date: ¶0-20-15 Survival and Growth.Data Day CONTROL - 10.7% " 21.4%
_ _ _ _ _ _A B C D E F C H I J K L 0 10 10 10 ]10 10 10 10 10 10 10 10 10 1 0 1bl* ~0 *, 0 10 t0O 10 10 tq
- 7, I0 *O O i 10O~ t 1tO (0 CbO IQ
"° Ir i0 1 0 10 JO 1010 lb i0 1O
- 6 1 10O '0 10 1 0 #0 #0 t 7D l0 ID II Q ' 1 0 10 ! 0 ijO 0 1 A= Pan weight (mg)... ... I ....
Tray color code:; tAY*,e., -CA'..fl' Analyst: ___ ___ ___ ___ 1 {.IIeo %.010 I(,*,* 14, 2..
I C.'.I l*.14 1(45.$* . t l%.'l 1i*.t"
- 7* 7 *2 Date: t9o - 2_.g -
B = Pan + Larvae,weight (nig)'. *.
Date: ___ -____ ____ ____
C --Larvae weight (mg) =--B - A Analyst: .*
Weight per initial number of larvae (mug)
= C / Initial number of larvae Hand calculated. C,.
I, '0 Analyst:. /3
= = I= 3 Average weight per Percent redaction iniia nmbe o Ifrom control (%) *o.,
larvialnne(r of _____
Comment codes: c --clear, d = dead, fg =fungus, k = killed, mn = missing, sk = sick, sm = unqusually small, ig =unusually large, d&r = decanted and returned, w = wounded.
Comment: v
. .. SOP ATI2O - Exhibit AT2O.3, revision 11-01-14 fi rage *z oT *
.... Page 3 of 6 Species: PimephalesDromelas Client: TVA I Seqiuovali Nucle~ar Pian[, Outfall 101* UV-treated Date: 10-20-15
____________________ _____ _____ _____Survival and Growth Data _ _ _ _ _ _ _ _ _
..... Day - 42.8%7 -a8.6% -, - 1oo% , ..
. _...._ _...... _ -_M .. N 0- P ... Q . R 'S., T U _V W X 1o o l l0b 10 lO toO 10 lO lO 2
__________ 10
- lk* 10 0 II) 10 I0j 1t* 10 to I* 1(
S !0t t0 t0 to. tO' to tO * (0 tO tO t
- 6. *0 f* t10 l* 10 10 tO '0 1* 80 7
A=Pan weight (rag)
Tray color code:: *,iWV1 £ GI tll IFI B = Pan + Larvae weight (Dig)
Date: l-*)fg-j*- u5*.
C =Larvae weight (mig) *-B - A ...
HncaultdAnalyst: A Weight per initial number of larvae (Dig)
= C /Initial number of larvae -,.* * *0 * .@ **,* *, @ .'
Hand calculated. * " * ° b * °
- Average weight per Percent reduction initial~number of. fromcontrol (%) *
- o* !.' **
Ue'a°* -*oa7i :
larvae (nrag)__________ ______
Commnt cnodes: c = clear, d = dead, fg = fungus, k = killed, m --missing, sk = sick, sm = unusually small, Ig =unusually large, d&r = decanted and returned, w =wounded.
..C..... m e t:, . . . . "' 2. . . .L SOP AT20 - Exhibit AT20.3, revision 11-01-14 53 of 98
- ...... Page 4 of 6 Species:
Client: TVA Pimeohales mn'omelas
/ Setusoyah Nuclear Plant,.Outfa~ll iDE UV-treated Date: 10-20-15 Day 10% Intake
_______ ___ Y Z AA B 0 10 2 i
'7o10 A =f Pan weight (rag)
Trayceolorecode:: ]rt)O.-L ]lLa..
Analyst: __________ I1 SO~
1C*.4L SDate: O'P2A.q-1i5 B- P'an + Larvae weight (mag)
Analyst: ., .*$ 5 C =Larvae weight (mag) = B - A Hand calculated* ~
Analyst:
- Weight per initial number of larvae (mag)
=f C /Initial number of larvae Hand calculate* .,
Analyst: *
- Average weight per IPercent reduction from control (%)
initial number of 0 **
Comment codes: e= clear, .d = dead, fg = fungus, k = killed, mn = missing, sk =sick, sm = unusually small, Ig --unusually large, d&r = decanted and returned, w =wounded.
Comments:" -.
R,~q,,,by
~MIoyE ,,.n SOP AT20 - Exhibit AT20.3, revision 11-01-14 Page 54 of 98
TVA / Sequoyahi Nuclear Plant, Outfall 101 U V-treated October 20-27, 2015 Pimephalespromekis Chronic Whole Effluent Toxicity Test EPA-821-Rl-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc.
)Preject unumber: 10958 Creeraltre 1uu%) Rel~cate Intltdmarbeel14d reu m rer-orl e A PmmweteJht*ergB- Pan÷Larvae tLenenenht(umgl Weighl/Ssucnvim tteruwetght/t Certflehatefse~aisb Wrtcht/1n.itiat mber Mea,,srtval *Mea lgIht/lteVl Ont.otiret Peresett ,dsetierfrem larvae weight~egl -A-B nuaerohtlccveelmgl SuumeigenuI'eof *m~P~I n 01alts-erer(g). (I%) neambeoflanve. -.aeathrn a..,eagc conatint ('.)
tern (erg ,=,...ron...tt%) (ag) Pea.jcer .O.,,.ct A I0 10 16.81 " 22.74 5.93- 0.593 0.593 Contcal B 10 10 16.06 22.06 6.00 " 0.6020 0.581 8.1 0.600 100.0 0.581 8,1 . Not appllcable C 10 10 15.27 -21.45 6.18 0.618 0.618
________ D 10 10 16.13 21.25 5.12 0.512 . 0.512 _ ________________
8 10 10 14.72 20.1I4 5.42 "0.542 0.542 0.% F 10 10 16.11l 23.06 i 6.95 0.695 0.9 200.69510.0591.0-.
7%--. G 10 10 16.66 22.32 5.66 0.566 0.9 20 . 0,56610.D.91.0-6 10 '0 1.6.35 271.95, 5.58, 0.558 _______ 0.558 ____ ______ _______
I . 10 10 16.36 22.91 6.55 0.655 .0.655 2140 Jr 10 10 15.97 21.70 5.73 0.573 066570.57310. 0.657-4
'14K 10 10 16.36 22.:33 . 5.97 0.597 0.0 70.597 100 06657-.
L "10 10 16.24 22.23 5.99 "0.599 _______ 0.599 ____ ______ _______
M 10 10 16.30 22.77 . 6.47 0.647 0.647
- 4. " N 10 10 16.19 22.36 6.17 0.617 0.0 290.617 10O 0671. ,.
0 10
- 10 15.71 20.65 4.94 0.494 0,611290494 100 067 29..
________ P .10 . 10 16.06 22.76 6.70 0.670 .0.670 ____ ______ _______
Q 10 10 . 15.98 21.57 5.59 0.559 0.559 85.l R 10 10 15.19 21.33 6.14 0.614 .91540.614 100 05154-.
856 10 10 15.95 21.63 5.68 0.568 0.9-. 0.568 100 05084-.
____-_____ 0 10 18.45 22.68 6.23 0.623 _ _____ - 0.623 ____
'U 10 10 16.75 . 22.88 6.13 0.613 0.613 10P .V 10 10 16.34 22.43 6.09 0.609 068700.609 100 06870..
10"a W 10 10 15.00 21.77 6.77 0.677 0 .61. 0.670.0 0617.0..
________ X 10 10 15.88 21.61 5.73 0573 ________ 0.573 _____
Y 10 10 15.79 21.82 6.03 0.603 0.003 00 Itke Z 1 10 16.41 21.72 5.31 0.531 0.531 100 0*861Z l taake AA 10 10 15.04 20.51 5.47 0.547 0.568 6.1 0.54710.0566.22
________ BB 10 10 16.06 21.97 5.91 0.591 _______ _______ 0.591 ______________
Out fall 101: MSD = Minimum Signific.nt Difference Dennett'S MSD valom PMSD = Percent Minimum Significant Differecee 0.0919 PMSD;r 15.5 ?1SDf is a oreasure of Sectprecision. Thal'MSI) is 6,e minimumr percent difference between the control and treatment that can be declared statistically signific.ant ina whole effl'uent toxicty test.
Intale:
Dunnettea IbSD value: 0.0566 Lower 1'1.SD bound determined byUSEPA (l0t], percentile) - 12%.
PMSb-D:" 9.7 Upper 8'MSD bormd deteried by USEPA (90th percenttle) = 30%.
Lower and upper PMSD bounds Were determined tfrott the 10th and 90th percentile, respectivey, of PMSI) data from EPA's WET Interlahorelory Variability Study (USEPA, 2001 a; USEPA, 2001b).
- USEPA. 2001a, 2001b. Final Report:Irtterlaboratory Variability Study of EPA Short-torn Chronio and Acute Whole Effluent Toxiceity Test Methods, Volumes I and 2-Appendik EPA-821-B-01 -004 and EPA-821-B-01-t05. US wrtioenmental Protectqion Agency. Cincinnati, 0H1 File: scqn1011 02015fdata-uv.rdsx Entered by: J. Sut~8r Reviewed fby:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated o~o T October 20-27, 2015
~Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 10120/2015 Test ID: PpFRCR Sample ID: TIVA/ SQN Outfall1101 End Date: 10127/2015 Lab ID: ETS-Envir, Testing Sal. Sample Type: DMR-Discharge Monitoring Report Sample Date: October2015 Protocol: FWCHIR-EPA-821-R-02-013 Test Species: . PP-Pimnephalespromelas Comments: UV-treatad Cone-% 1 2 .3 4*
D-Control 0.6930 0.6000 0.6180 0.5120 10.7 0.5420 0.6950 0.5660 0.5580.
21.4 0.6550 0.5730 0.5970 0.5990 42.8 0.5470 0.6170 0.4940 0*6700 85.6 0.5590 0.6140 0.5680 0.6230 100 0.6130 .0.6090 0.6770 0.5730 Intake 0.5030 0.5310 0.5470 0.5910 Transform: Untransformed 1-Tailed . .Isotonic Cone-% Mean. N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5808 1.0000 0.5808 0.5120 0.6180 8.098 4 .0.5989 1.0000 10.7 0.5903 1.0164 0.5903 0.5420 0.6950 11.951 4 -0.249 2.410 0.0919 0.5986 1.0000 21.4 0.6080 1.0435 0.8060 0.5730 0.6550 5.732 4 -0.652 2.410 0.0919 0.5988 1.0000 42.8 0.6070 1.0452 0.8070 0.4940 0.6700 12.9;15 "4 -0.688 2.410 0.0919 0.5988 1.0000 85.6 0.5910 1.0176 0.6910 0.5590 0.6230 .5.444 4 -0.269 2.410 0.0919 0.5988 1.0000 100 -0.6180 1.064'1 .0.6180 0.5730 0.6770 6.999 4 -0.977 2.410 0.0919 0.5988 1.0000 Intake 0.5680 0.9780 0.5680 0.5310 0.6030 8.068 4*
Auxiliary Teats Statistic Critical Skew Kurt Shepiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98963 0.884 -0.0913 0.47811 Bartlett's Test indicates equal variances (p.= 0.81) 3.5619 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChiV TU MSDu . MSDp MSB MSE F-Prob "d Dunnett'sTest 100 >100 1 0.09189 0.15823 0.00076 0.00291 0.9287 5, 19 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(E~xp) Skew ICO5 >100 1C10 >100 I015 >100 .1.0 IC20 >100 0.9 1C25 >100 . 0.8 IC40 >100 I050 >100
- 0.7 0.6
- , 0.5 0O.4 0.3 0.2 0.1
-0.1
-0.2 . . . . . . . .
0 50 100 150 Dose %
Dose-Response Plot 0.7 0.6 0.5 1-tail, 0.05 level of signilicance
- 0. * .
0 - N 10-0
. File: qsq101102015 data-uv.xlsx Entered by: J1.Sumner Reviewed by: _
aae 56 of 98
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake
- UV-treated October 20-27, 2*015 Statistical Analyses EnvIonmsntal Testi ngSobjilons, Inc.
Fish Growth
- Sampleand Survival Test-7ID:
Day Growth Start Date: 1012012015 Test ID:LarvalPpFRCR TVAI SON Outfall1101 - Intake End Date: 10/2712015 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: October 2015 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pirnephales promnelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5930 0.6000 0.8180 0.5120 10.7 0.5420 0.6950 0.5660 0,5580 21.4 0.6550 0.5730 0.5970 0.5990 42.8 0.6470 0.6170 0.4940 0.6700 85.6 0.5590 0.6140 0.5680 0.6230.
100 0.6130 0.6090. 0.6770 0.5730 Intake 0.6030 0.531.0 0.5470 0.5910 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Mini Max CV% N t-Stet Critical MSD 0-Control *0.5808 1.0000 0.5808 0.5120 0.6180 8.098 4 10.7 0.5903 1.0164 0.5903 0.5420 0.6950 11.951 4 21.4 0.6060 1.0435 0.6060 0.5730 0.6550 5.732 4 42.8 0.6070 1.0452 0.6070 0.4940 0.6700 12.915 4 85.6 0.5910 1.01 76 0.5910 .0.5590 0.6230 5.444 4 100 0,6180 1.0541 0.6180 0.5730 0.5770 6.999 4 Intake 0.5680 0.9780 0.5680 0.5310 0.6030 8.068 4 0.437 1.943 0.*0566 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.8848 0,749 -0.9219 -0.3332 F-Test indicates equal variances (p = 0.62) 1.8616 47.4583 Hypothesis Test (1-tall, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic tTeat indicates no significant differences . 0.05665 0,09755 0.00033 0.0017 0.67716 1, 6 Treatments vs D-Control . .. Ds-epnePo fIR
- 0.7-1-tall, 0.05 level
- 10.5 of significance 19 0.4, 00.
0.2E 0.1 04 CD 0 0 ~-
(SI e~J
.~. CD -
Cs File: sqnIl01102015data-uv.xlsx Entered by: 3. Sumner Reviewed by:
57 of 98
C) ~
~
Page 5 of 6
.Species: Pimenhales promelas Date: 10-20-15
- Client: TVA / Seau~ovah Nuclear Plant. Outfall 101. UV-treated Daily Chemistry:
[ Aaytidentified for each da, , performedpH, D.O0 and oonductivity, measurements only.)
1_____Lls oil (S.U.)...... ~.O5 II 1.95 IJ.~1'i b19 I-l.Vl5 iij.qi IIt======.J.
r---- r -a - ______
DO (mglL)
Conductivity Laa CONTROL *Mkalinity -
UV-treated (mg CaCO3 /L)
(~+/-mhosfcm)
- Hardness 57~ -
(ing CaCO~1L)
- Temverature (0C) 2.'AA ~i'L4.¶a 'i~4A Fi~7W - v L pH (S.U.) II -7 ~ c~7i e.oO 171 Ict~][
I-I.
DO (ngdL) 10.7% Conductivity mhos/crn _______
- Temnerature (°C) a.
~V2- I - -
14A.. N pH (S.U.) =4~I
- 00 II-! .I *!- I1.c-*
-~
II 7'* I DO (ma_/L')
21.4% Conductivity
_(*imos/em)
- Temoerature (°C) ~O L. a i . -- = - _. _!, -- * -- ..
- plJ(S.U.) Ii-q7 B?~W N DO fm*L) -I 42.8% DO (maiD Conductivity N B.
(i+/-mhos/cm) o'.25
- Temperature (0C) LS .O
.1 'U. -~
oH (S.U.I .0I
...... -- II----------
!DO (mWL) . I' -I S5.6% Conductivity hemhoslcm) -
1.o 4
- Temperature (°C) 4 1~~A *-1~
nHl (S.U.' II 1..Qt.~ I - -- "Ul* Vm*=ml=mmll==t!
DO (maiDL A a
Conductivity (amhos/cmn)
.5 I1~3 100% *Alkalmnity S*Hardness*
]*TR chlorine (rag/.)
- Temoerature (°C)
,60I-~
4 -7 pH (s.u.)
Conductivity 7.* .. %C 7..
100% *All~lifiuty*
Intake ~ gCC3L
- Hardness "
,dmtperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
/R,*.-*kinlity* hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet b.
XoI'o cs..* . _.Total residual chlorine was performed on non-treated Outfall 101 andlIntake samples.
\ oIK,,98" -*age 8 *SOP AT20 -Exhibit AT20.3, revision 11-01-14
...... Page 6of 6 Species: _Pi.meyhales pr'omelas Client: TVA / Sequovah Nuclear ]Plant. Outfil~l t01, UV-treated Date: 10-20-15 Day M
a la (Analyst identified for esoh day, performed pH .O ndcodctivit measurements only.) H I B Ana1ys~
Coneen-tration IParameter pH (s.u.) _ 'i -i-. I DO (mr_/L)
S- II- -i--A-----
Conductivity CONTROL (umhoslcm)
UV-treated *AlJtalinity (ma CaCQiIL~ _
- Hardness (inc CaCOSL')
- TempDerature (0 C)
DO(rng/L)
!nhos/cm
- Tern erature('Q ~. A
- 1,~SI.t~ 1. ~6 L ~ jJ j,4 ~ 4 C2.r*n I LA 1 ~MjL7q~J~LJ DO (mrIL) 17* r 74' 21.4%
Cond:ctivity
- Temmnarature (0 C1 I ~
II ,A..u, I ,~i II ir~i-~-
DH (S.U.) II 1.10 I %.O1 II Q~ 1 ~ II 7.9* I a304*
L 11 $ - H DO (mWL) li tl 42.8%
I Conductivity (j+/-nihos/cm)
- Temnerature tPC) ~S.O -IA.-t udkmmmmimalma* ...
isa~
.. [*a IpH (S.U.) 0 lv~ ~IA~ Ii - - I II L~T - -
DO (mgfL) 85.6% Conductivity (i+/-mhos/cm)
- Temnerature (°C*
9 -. - ~ - ~*--. ~m....
DH (S.U.) 8a03 II 7A~* I J.92 DO (mg/L) 7',
I Conductivity I~3
- AUwlinity U 100% 15
~HardnessH
~Th eblouine (mg(L) ~co.lO K. I
---i --- -- _ ,,
"1-91 II 7* .0 Conductivity
- Aflkalinity
-Iso 100%
Intake
- Hardness*
- TR chlorine (mg/L)
'A oow
~t I -aD ~ ,~ ~q~*
- liem eratnreru) 4*.t ' *) " ** L ..*2 I -a £... *.5*. ,
- peratures performed at the time of test initiation, renewal or termination by the analyst identified inthe Daily Renewal Information table located on Page 1.
I(!r.yo* jk51 ity, hardness and total residual chlorine performed by the analyst identified onf the bench sheet specific for each analysis and transcribed to this bench sheet
- __.. Total residual chlorine was performed on non-treated Outfall1010 and Intake samples.
"-- Page 59 of 98 " . "SOP AT20 - Exhibit AT20.3, revision 1 1-01-14
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated
~October 20-27, 2015
¢DW E. r *l**Pimephales promelas Chronic Whole 1Effluent Toxicity Test
~EPA-821-R-02-013, Method 1099.9 0
- Daily Chemical Analyses Project number: 10958 ia~ A EnvironmentalTesting Solutions, Inc.
ConcentrationParameter DaO Dal Da2 Da3 Da4 DaS Da6
____________ ________________________ Initial Final Initial Final Initial . Final Initial . Final Initial Final Initial Final Initial Final pH (SI]) 8.05 7.95 7.99 7.79 7.95 7.91 7.99 7.69 7.99 7,64 8.12 7.92 8.03 7.80 DO (malL) 7.7 7.7 78 7.7 7.7 7.9 7.9 73 78 6.7 7.9 7.7 7.8 7.8 Conductivity (junhoslcm) 311 325 308 32.5 302 313~
Control Alkalinity (mgJL CaCO 3 ) 63 63 63 .
Hardness (mglL CaCO3 ) 86 86 86 ~
Temperature (IC) 24.9 24.6 24.9 24.4 24.9 24.5 24.8 - 24.7 24.9 24.6 24.8 24.4 24.8 24.6
~H (SLY) 8.05 7.92 . 8.00 7.74 7.96 7.90 8.00 7.69 8.00 7.60 - 8.13 7.93 8.03 7.80 10.7% DO (maiL) 7.8 7.7 7.8 7.7 7.9 7.9 7.9 7.3 7.9 6.7 7.8 7.8 7.8 7.7 Conductivity (junhoilcm) 298 '. +/-.. . 312 ,.. 295..............310 291 300
___________ Temperature ('C) 24.9 24.6 25.0 24.4 24.9 24.6 24.9 24.7 25.0 245 24.8 24.3 24.9 24.6 nH(SU) . . 8.05 7.92 8.00 7.74 7.96 7.92 8.00 7.72 8.00 7.61 8.14 7~93 8.04 7.82 DO (malL) 7.8 77 7.8 7.7 7.9 7.9 7.9 .73 7.9 6.7 7.8 7.8 7.9 7.8 21.4% Conductivity (amlaoslem) 284 . 29'l 278 ~ 294. 280 ~.7. 286 298 ~
____________ Temperature ('C) 25.0 24.3 25.0 24.5 25.0 . 24.6 24.9 24.6 25.1 24.6 24.9 . 24.6 25.0 24.7 8.05 792 800 7.74 7.97 7.91 801 770 801 753 815 792 804 778 42.8% DO(EII~IL) 7.8 77 78 7.6 7.9 7.9 79 74 79 60 79 79 79 78 Conductivity (jembos/em) 257 26~ . .j~ 251 , 26(j 260 275 ~
_________ 25.0 244 251 24.2 25.0 24.4 250 247 251 246 249 244 250 246 pH(SU) . 8.05 795 801 7.75 '7.98 7.89 802 765 801 754 815 792 806 779 IDO (maJI.) 7.8 7.7 7.9 7.51 7.91 7.91 7.9 7.3 It i llmfl fkMIEIRI I . c.nI .~ Al~~J I85.6% Il*flHIIH*IIVIrV
]Temperature (°C) r" __ ___
IpU (S'U)_
IDO (meILl 4-Conductivity (atmhos/cm*
100% ' Akalinity (mglL CaCO 3) __
arn,*-ess (ma/L CaCO 3)
'To~taL Residual Chlo~rin (malL)
Temner*hle* (o[-* 25.0 24.4 25.3 24.4 25.2 . 24.4 25.2 24.5 25.1 24.3
____________ -, 4 - 8.02 7.77 7.97 .7.71 n.TI (Q'I'] 8.08 7.96 8.00 7.84 8.00 7.92 JI--..---
DO (ma/L) 7.8 7.8 8.0 7.6 . 8.0 7.9 7.9 7.3 7.8 6.8 Codctvty (junhos/em I 174 . . 178 177 187 180 100% Intake Alkalinity (mg/L CaCO*) 69 . 71 73
[Hardness (ing/L CaCO 3) .it------- 61 . . 65 69 hlrn
.iua fm Il ~0.10 - ., <0.10 . <0.10
. .tl .
Temnerature ('Cl 25.0 24.6 25.2 24.5 25.1 24.5 25.1 24.5 25.1 24.6
'Note: Total residual chlorine'was performed on non-treated Outfall 101 and Intake samnples.
File: sqnlOlj_120l5chem-PUV.xlsx Entered by:" J. Sujnner Reviewed by.
PagejLof ~
'1rotal Residual Chliorine (ORI0N-1977)
Matrix: Water,RLL= 0.10 mg/L Meter: Accunmet Model AR25 pH/Ion Meter
- .!. Analyst __ __ __ _
' -Iodidereagent:
'i".. Date analyzed LO -. i- i Acid reagent 0.10 mug(L [ 1.00 mn ,/L II(sunnestedmV Change ranne = 26 to 30 mV* I Millivoits(mV) I0 "q*,*
- h *-3 'j , "-,
N,ote: For samples with a residual chlorine of'> 1.0 rag/L, the samples must be diluted to be within the calibration'range.
Laboratory¢controlstandard:
Reference standard True value (TV) Measured value (MV) %hRS = MV/ TV x '100 1 number (ragfL) (mg/L) (acceptable range 90 to 110%)
I st4*S.*'5 0.50 'O.,r> i ,, o5. yh7J Duplicate sample precision: ___,__,__,__*____"__ .
- number " " *. L) (acceptable range - ' .. .:%
4 Duplicate D 0 0 e.
- Sample measurements: __________________, _______
"'Sample S pIe'D. Sample "characteristics Reslidual chlorine
,Reagent Blank ... O. ooo'4*
'Ood3 CAC,.o,.V *o C~0*o *o .0ooo*2...
- 'o2o..O' O~~r c~e*-,~~ catou.* (o.oooQ1,,V Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboritory controlstandard:
Reference standard True value (TV) Measured value (MV) .% RS .- MV"J TV x .100 number (rag/I.) (rag/L) (,acceptable range = 90 to 110%)
.. *3
- f 0.50. 0.1 ,C-647& ,I°/
Reviewed by E[ IZ""Z*
'...- ,,!. ': ...,:*" : . Date reviewed L1* .7-,i*" ."J
.. -,,S -:-
SOP C8 - Exhibit C8.l, revision 01-01-13
- ii*of 98 I I I Ill II I I I I = , ,
Page
- Page __. of j I Solutions, Inc.
Total Residual Chlorine (OBJION-1977)
Matrix: Water, RL = 0.10 nig/L Meter: Acecumet Model AR25 pH/Ion Meter h
Analyst Date analyzed [ -5 ,
[.j.I 7 j-j.- °dide reagent: I*
Acid reagent:. LI iIZ.'iq ?t 0.10 mgfL [ 1.00 mg/I, I mV'Change I(suggested- idmee.". 26 to.30 mV) iiReference- -tandard umbe*)r,, ..... i[ *.
Millivolts (mV)' 4",t*Oj) _, $5*
[,IS**",
a6,-* o04.'* J--*- . .",;!*i.i.. -
Note:.Forsamples with a residual chlorine of > 1.0 mg/L, the s~amples must be diluted to be within the calib~ration range.
Laboratorycontrolstandard:
- r. Reference standard number True value (TV)'
(ragTL)
Measulredl'value (MV)
' (mgIL)
% RS. = :*' (TV*..,100 I(aeceptable range *9.0!to11'%)
Sample T Sample ID Sample characteristics [Residualm/chlo°rine %]D(eeta!:inN
%RI ... [. .+/-*.12J)~x .,-.....i
)s 00"...s
- ={(S number .*'.
Sj Duplicate " vtvt, -----.. '.-'*.,",
Sample measurements: ,,"":.. " '**'. :*
Re:.,a.. ent. Blank;.:..:. ;
S~ample. Sample IDSapl charuacPtestcs L ...: "!,!4q*li~e
___....:__ ___:______ _ ,CO*O*o,',f*..'
161 2A.*c, t ka, ' ",10F * "O Note: All samples were analyzed in excess of EPA recommended balding time (15 minutes) unless otherwise noited?*.)**L!'. ..;'
L r*aborato-y controlstandard: "":'"!):: '
Reference standard True value (TV) Measured value (MV) dR'S M. iTV ~0,0*
.%fi
! *number I(mg/L,) I(rag/L) (aceptab* range ;29O'to 110%)
! Date reviewedLL II 1
! SOP C.8 -Exhibit C8.1, revision 01-01-13 Page 62 of 98
Page ')___ __
Page
- of I I 0 a
e II Inc.
envunmeiuaiiasttna Sohatiuns, Ii'.. Total Residual Chlorine (ORION'1977)
Matrix: Water, RL"O .lI0mg!L Meter; Accumet Model AR25 pH/Ion Meter
- I. Anayst FWii6 Date analyzed "[ii, q,-* I 3,
I odide reagent. ((*I Acid reagent: *!7* *U (j7 II 0.10 mg/L " 1.00 mg/L mV Cha.ge j I.' Note: For samples with a residual chlorine of > 1.0 mag/L, the samples must be diluted to be within the calibration range.
Laborator con~trolstandard:,... " III I' Reference standard True value (TV) ". Measured value (MY) % RS = MV / TV x 100 number I(m _g/) ... (ng/I.) . (acceptable range = 90 to 110%)j S~u II)*
[..nmeSample*.*'.*lDpiameSple SamPle. chara~cteristics._
. _.. S*'/Residual
,, m/Lchlorine. %P ( )/fSDl] 0
~1 I Sample measurements:_______
Residual clijorine I
SapeSample ID "Samplecharacteristics Reagent Blank ...... *, o*c*l I,
I Ii I.
Note- All samples Were. analyzed in excess of EPA recommended, holding timle(15, minutes) unless otherwise noted.'
'I
- Laboratory controlstandard:. ,
Reference standard . True value (TV) IMeasured va~lue (MV) %RIS =MVI/TV x 100 I number , (rag/L) I(rag/L.) *(acceptable range = 90 to 110%)
II Date reviewed /L *.2 t I,I SOP C8 - Exhibit C8.1, revision 01-'01-13 Ij Page 63 of 98
Page ______
Page 1 jof ~
lng SvIutIonsIric.
Mkal*]iint (SM 232) ]B-11997)
VMatr-ix: Water, RL = 1.0 mg CaCOO/L Time__initiated.
Dii:
ate analyzed ll=**,D Titrate samples to pH =4.S S.u, and mrnulilierdetermination:
~1 -~ = = = = ==
Normality Normality (NY) of H2 S0 4
- cheek Begin End Total =(5 ml Na 2CO3 x 0.05)IE* fi(Nx 50000)1/100 ml sample standard ml ml = 0.25/E =Nr 500 rnnmhar 'aceeentable ranee = 0.0180 - 0.0220*
_Laboratorycontrolstandard:.. . ....
- Reference standasrd Trure value Sample Alkalinity (MV) % 11$- yv/TV .xIODO number (TV) *~volume .Begin End Total Multiplier. (rag CSCO3IL) (acceptable range 1Dupllcatesample preZ!on: : ........ init Sample Sample ID volum~e 1 egin
'_________._ (ml) ,ml j End ml Total Multiplier (ng CaCOa/) I (s-D)1l(S+D)P]}x00~
7 iijjjj.. j .ge.. =j. )
Matrix spike reccl~rv jReference standard Spike value Sample 0 r _______.....__
Spike alkalinity (A) 0-fl number (SY) volume Begin End Total Multiplier (rag CaCOs/L)
~ ~(mi ziac~OgL) (ml) ml, ml ml ___________
...:Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 !
(rag CaCOa/L) MV A A- B .(acceptable range (racotaiO- =75to 125%)
S* le measarements ......... ........ _____
Sample volume Begin End Total .Alkalinity Sample number Sample IDml _ml. ml ml "Multiplier (mg CaCO.*
m*** _____ 157 )qa 3'.3 - __... _
______.___ Os*.(t *r. ____ __ __ __
Reviewed by: j~j~~jJ
- Date reviewed~ %D-a~. IS Page64 oC698SOP
- Exhibit C6.l]. revision 01-G1:13-
r~gv ________
Page _______of______
1 ~~
FmdfD~1meflt3ITGS~SOIut~o~sIRc.
Matrix: Water, RLL = 1.0 mg CaCO3 IL
-Time i'tit Time completed Date analyzed [7. j
- Titrae *smples *o PH = 4.5 S.1LL 7Wan~f wnrnialitt, und n*ulthoio. dojmv*irndo
- '-Normality N..
ormality (Nv) of H1S0 4 -==pH Factor or Multiplier ..
Deionized Titrant" Begin End Total - (5 ml Na 2CO3 xt 0.O5)/E = (Nit 50)000)1100 ml sample water reference sizM~ard ml* ml = 0,25/E *Nit500
= 4.5* S.U. number "number ,, (acceptable range,= 0.0180 - 0.0220) _____________
efrence standar-d Tr..ue va*lue ..... Sample ....... .... A lalnity (MV % RtS --MV / TV it 100" Rfenumber (TV) volume Begin End Total IMultiplier (rag CaCO3 /L) (acceptable range (mngCaCOsIL) (ml) ml ml ml I = 90 to 110%)
Dnplicate Sample precision:"__*__ __ ............. ___
- eee**anadsievlenSample A.. lkalinity pk %(PA)
Sampler " (pb- volume Begin End Total Multiplier (gCClL (Sa .DO~)./(+)2110 nube Irgt a~L (ml) . ml ml , mi ____ _____ (cetbenne~1%
Dulct (mg 1a71/ MV D 73-____aep___lera__
M ari spike resovem i s:
SnampltavardumSpikgvalue SapleTSpike Alkalinity(A Sapenumber (Samp vlume Begin End Toa l Multiplier ( g C~a.COiL rn )
(i"ng Ca,,,L ml m l m
[tefer .e _*... f I2. -3*.Q*,*30 *3,6 Reviewed by: [ ADate reviewed: *' )i-.*. l&
SOP C6 - Exhibit C6.1I. revision 01-0]- 13
Page
- of *
~,
Em~mJT0t1i.gS~Evons~Jn.
.Mk~Aini* (SM 2320 J*- 11997)
Matrix: Water, RL =1.0 mg CaCO3JL Time initiated Date analyzed [ , TIritrate sa*mpltCS to Time completed [7j7-* '7 pH *-4.$ SoU°T kreanirvl M, gujdml irnia dn jad dulnlo*rntbd Deinizd NrmaityNormality (N) of H2S0 4 p H Factor or Multip~lier eined Titrant ec.k Begini End Total =(5nl Na2 CO x 0.05)/E *(NxS50000)/100 ml sample water, reference stan* ml ml ml =0.25/B =Ni 500
--4.5 S.U. number number ** J L) ..(aereptable range - 0.0:180 - 0.0220) _____________
-- eeeneestaudard "Truevalue Sample .. .......... . . .. Am~lkaia ( %*MV+)S V/* 100 number (TV) volume Begin End Total Multiplier (mg CaCOsJL) (acceptable range (rg caC*/IL) ) ml .1 ml. ml . .-90 to110%) ,*.
Sample
- Sample ID volume IBegin End ITotal IMultiplier (mg CaCOg/L) + ((S -D) I[(S+D)12]~} z 100 Dl .& (a5:eeptcaboe rSmule 10%)
Reeence standard* Spike value Sample Spike alkalinity (A) number " (SV) volume Begin End Total Multiplier *(mg CaCOjIL)
- I (mngCaCOs/L) (ml) ml ml ml Sam+le measurements: ____...._
....... Smpivolume "Begin Enmd T...*otail ........ .. Aikainity Sample number Sample I'D .(mr} ml .ml mlI Multiplier - maCaCO/L) amo+, e.oj __"_"_____
Reviewed by:I Date reviewed: [- ! .**.f SOP C6 - Exhibit.C6. 1.revision 01-01 -13 Page 66 of 98~
Page ~
Page SD!UUgnsIr~c.
EnvimonlentolTeitblg Ailkaifnit (aM 232013°4997)
Matrix: Water, RL 1.0 nig CaCO /li3
[7~U77~7~~ Time ini*I7
-~ Analyst Time completed [I-I~I Date analyzed ~ Titrate samp~es t*o SpH = 405 S.U, lWwrnt nawnvnlhn nnoA 11inlhi~ ,lfADflNIflOfBflP H oxf -Normality -Normality (N) of YJI2SO4 pH Factor or Multiplier Deion*e at check Begin End Total = (5 ml Na2CO3 x 0.05)/B (N x 50900)11I00 ml sample
- water .reference -l~~drd niml ml ml fi0.25/B --N x 500
= 4.5 S.U. number numberr" ___ J*) (acceptable range = 0.0180 - 0.0220) ____________
Laborago controlrtandard: *______________________
efrence standard True value Sample .JAlkalinity (MV) % ItS =MV/TV x 100 number . volume. B*egin End Total Multiplier (uag CaGOs/L) (acceptable range (nig CaCOa/L) (ml) ml .ml ml ""- 90 to 110%)
Di~lcate-sa ~le recisoia:....... S m l [... j ' . . ..A k lnt [
- 0 -..... .
Sample SampleZHI volumie B egin 1E*nd [Total }Multiplier (mg CaUOsIL) R-D) /[(SD)/2]} il00
{(
number ml _____m____ ml____l__a_______e_____=___0%
Matrix spike recover, : ._______________________
Rieference standard ...spike value Sape......Spik alkalinityi (A*)...".....
numbe~r (SV) volume Begin End Total Multiplier (rag CaCO3/L)
(rag CaCO3IL)...mi ml ml ml II Sample alkalinity (B) ' .. Measured spike value (MV) %R
% = 1*/-SV lo0 (rag CaCO3IL) MV = A - B (acceptable range
.,(mg CaCOsIL) =75 to 125%)
Samnple measurements: ._*_ ____......
- Saimple volume Begin En*.d iTotal .. ' j Alkli~ni ....
Sample number Sample ID '(ml) mE sl m:nl Multiplier [ (rag CaCOs/L)
_ _ _ _* _ , ~ ._
___. ____ os* =
Reviewed by: Date reviewed: Tl0-.t, S SOP C6 - xhibit C6.1. revision 01-01-13 Page 67 of 98
a Page ______
Page
~ whunmentalTesttng5o!uklons.Inc.
AllaInity (SM X32A) B-31997)
Matrix: Water, RlL =1.0mg CaCO3 IL Time initiate*. 777[
Analyst Date analyzed O i i* " i Time completed ]I I .I~iII Titrate *am~p]an to pH = 4.5 S0IL.
Dnplicatesa* pe preeison: ______ _________
sample Sample XD. volume IBegin ]nd E Total .Multiplier (migCaCOsg) (('D){ J(S.-D*ti")/*]} ilU number_ __ _ .__ (ml) .ini ml ml _ ___g_
Mfatr~i spike recovery: ...... __________________.__
IIReference standard Spike value Sample .... ..... Spik~e alalinity (A) ......
number (SV) .volume Begin End Total Multiplier (ing CaCO*/L)
(mug CaCOs/L) (ml ml ml nil..
(rag CaCOzIL)
S(mag MV = A - B CaCOs/L)
(acceptable range
=75 to 125%)
A Sample measuremenis: .............. .. __ ____________
Sample volume ... Begin End Total Alkalinity Sample number Sample ID _.(ml) _ ml ml ml Multiplier (ma CaCO3 /L)
- o*.* ,* *_! __ ,*.Q *,j S. _,__ .
Reviewed by: Datereviewed: [, b.1.ISL SOP C6 - Exhibit C6.1. revision 01-01-13 Page 68 of 98
.Page 7 0
~ E'~vIr~nment3~Te5tIng Solutloni, Inc.
. Hiardoess (SM 2340 C-i997)
RL = 1.0 mg CaCO /L 3 Analyst[ j7* Time initiated **
Date analyzed [ q* Time completed [*'*-jj Thrant ,*ormality and ,wdtplier detrminaio:
Ti-trant ... Normalitycheck£ Begin Endc Total ... Normality (J) of EDTA ...... pH D'actor or Mul'tiplier reference standard ml ml nml-- 0.2/15 = (N x 50000)/S0 sal sample.
number number .. (E) (acceptable range = 0.0180 - 0.0220) = N
- 100O Lasboratory control standard: ,__________,_________
1'apl'e
- Reference standard number f True value (TV)
(sag CaCOa!L) volume (mu)
Begin m
End ml Total ml 1Multiplier J=
Hardness (MV)
(rag CaCO.IL)
%RSMI*-= VTV 21@0 I (acceptable range 9Otol110%)
Duplicatesam *le precision: .
--- ' Sample . H..ardness.. .... % * :.....
Sample Sample ¶0 volume Begin End Total Multiplier (rag CaCOs-L) {(S -0 /1)(,541)121) x 100 number. ______ _ ml)~ nml ml nil ___
Matrix spike recovery: _________________________________
Reference standard Spike value Sample ... ...... Spike h~ardness ('A) number (SV) voumne Begin End Tota ul~tniplier (nig CaCO3 IL)
(ag CaC.O.3L) inl ni l ml Sample hardness (B) 'M'asured'spike value (MV%) ' % Il MVgfg/SV x 21008 (mug CaCO3 IL) ]V = A - B M (acceptable ranlge I (n.i g CaCO3 I.). = 75 to 125%)
Sample number Sample 110 (m1m ml ml M~ultiplier (rag C.aCO3 IL)
TV'ND(should beo= O.rg CaCOs/L) __
Jb°*"i * ' : .O ___ *7 !*O tl _ __ _ __
I Reviewed by: Date reviewed [ 4 Note: If >1 5m1 oftiirant is used, sample must be diluted.
Page 6C7of 98SOP
- Exhibit C7.1. revision 0 1-01-13
a Page
- I Page _* ~of 3 I bnmn~a1TesUngS~dut~wi~ Inc.
lHardness (SM 2340 C-31997)
RL =1.0 tug CaCO3/L Time initiated .
Analst Date analyzed Time completed [I I[I F
I?
Laborot ca Reference standardo _t lstandard:
. Tr'e value.......
Sample :. ...._ __ ......
H.. _
-ardness __ _
(IWV) __ __
"...%R _ __
='f ....
MVJ:/TV_ __
. 100 _
number (TV) volume Begin End Total Multiplier (inag CaCOjIL) (acceptable range
________(rag CaCOML) ( ml, m ml ml .. = 90 to'l10%)
D~ lcatesampleprecsion: .... . . .. . .. . .*-..... ... .,.: -
.... " .......... I Sam ple . . . .. .] Ha rdness. ..- "i _'* " .... .'
Sample SampleZ ]Tvolume Begin *nd ,Total Multiplier E (mg CaCOsFL) {(S "- .*9$0, number ______ .*L l m l___
ii Matrix spIke recovery , ,, , ___________
Reference standard 'Spike valiue Sample Spike hardness (A) number . (SV) volume Begin E.nd Total Multiplier ,(mg CaCO3dL)
_______O__ (mi) m!l~jL m l4
,Sample hardness (B) Measured spike value (MV) '% R -MV!SY xi100
! (nmg CaCOsIL) MV* ffA - B (acceptable range Sample volume Begin Egnd Total Hardness Sample number Sample ID (ml) ml *ml ml Multiplier (ma CaCOsJ)
TV ~ (should be-" 0 mg ______ - 4 -- 2*h*
D*I leO'1* -* __ ___ ___ - o- ').* o.
, *
- _.____, , i* ., _ __.._.. _ _ _ _
Note: If >15rml of titrant is nsed, sample must be diluted. -Reviewed by: I
- iiI Date reviewedI [o~do*
SOP C7- Exhbit C7,I. revision 01-01-13 Page 70 of 98
Page I*
Page -* of~
4~ I.wimIment3IT~stInD 5DtutIon5dn~.
JHlardmess (SM 234~O C-11997)
RL.= 1.0mg CaCO 3/L~
Time initiated * " 12 Date analyzed Time completed [I I* I Tk, antnormalit andmutiplier determination:*............
.... *-~rnt* * " otl Nornmlity (N*)of FDTA pH Factor or Multiplier refeencrn stndadml * " ----. *. _ _ * .... =(Nx 50000)!/50 mi sample number number CE) (acceptable range=.= Nix 100 Labondorj'contlvol standard: ________________________
eerence standard 3ue value Tr. ... Sa[ieHardness (MV- %1S = MV! FT x 100 number - (TV) volume Begin End Total Multiplier (mag CaCOsIL) (acceptable range
._Dup_ licatesam21e preciiOn: _______________
Sample . .. :* .. *Ha,r-dn-ess .......... %RPD = "....
BSample Sample ID volume Begin Ed.oa utpir(a aOL (-)[SD/] 0 number m). md
- n E*b** ta*l ( - "utpir(igCCsL
.)I(4D~)xil Matrix spike recovery __________"_________"____
.Reference standard Spike value Sample .... .. ... ... spike hardness (A) number (SV') volume Begin End Total Multiplier (ag CaC*sL)
' CaCOa/L)
(mg}*O* (m) nml ml ml (mgpl CaCOS/L) Meaue = A-B (acept~able range
-.. (sa CaCOs)L = st 125%)
S mle measurements:________
- Sample volume "Begin End' Total' Har.....
dness Sample number Sample lID fml ml ml ml Multiplier (mag C.aCO.3L)
TV ___- -... = - *
- _ - -3.*, *.* __ ___-
Note: If >15m1 of titrant is used, sample must hediluted. Reviewed by: [
- Date reviewed [ *4i6*.
SOP C7 - Exhi'bit C7. I. revision 01-01-13 Page 71 of 98
Sequoyah Nuclear Plant Biomonitoring October 20 - 27, 2015 Appendix D Reference Toxicant Test and Control Chart Page 72 of 98
- Pimephales promelas
@ ~Chronic Reference Toxicant *Control Chart
- *'* *Organism Source: Aquatox, Inc.
S Environmental Testing Solutions, Inc.
I.,o I I I I I I I l ! J I I l I ! J ,
i *PP* * -! - .
II .. L -__ It. = q __ _--_.--- -] I _ _*B. .
UM:VA Lonltrol LimitS *__L :tanaaro ueviatlons) 0,9 0,8 0.7 0.6 0,5 I I I I 1 I I I I I I I I 1.4 S I ] I 1 I I I I l I I ! I I I ' I USEPA Warning and Control *Limits, (75t and 90t Percentile CVs) 1.2 ..................................... *
- 1.
i>.0.6 "U L A 0.,4 1 I 1 .1 I I I I I I I I I I I I I I I I I 1.4 i J L I i I l Ii II I I I I i I I I I I Laboratory Warning and Control Limits'(10h and 25h Percentile CVs) 1.2 1.0 0.8 0.6 0.4 I I 1 I I I I I I I 1* I I I I I I I I I Test date
7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- Central Tendency (mean IC2 5 )
- -* Warning Limits (mean IC2 5 :+/- SA.10 or SA.75) Grahsg....ted frm ... soeited .... 1, spreadshaat.
- % .. .=:,'" Control Lim its (mean IL2 5 -+SA 2 ,S .0 or 2 Standard Deviations) Excelspreadsheetente dbv: l.Sum nrr aAA~5 ~.9'Reviewed by: *l 73 of 98
rsE Pimepholes promelos Chronic ReferenceToxicant Control Chart i-'** Environmental Testing Solution s, Inc.
0 Laboratory" Laboratory USEPA ' USEPA Stats and USEPA 5 Test number Test date 7-day lC* CT S Control Umits 5** Warning Unmits S*J Control Umetts $As5 Warning Urnits naas Control Uimits CV WIJ KcC) (g,/LCC) Cr-2s a+2s CT"-Sku CT+ u, r-5*s~ aT 5s2 aT-S41 s aT+~s5 a'"- 8 8 , C" 5*s..*,
I. 07-08-14 0.74 0,76 0.05 0.67 0.85 0.09 0.67 0.86 0.16 0.60 0.92 0.29 0.47 Los 0.34 0.42 Li10 0.06 2 08-05-14 0.88 0.76 0.05 0.66 0,86 0.09 0.67 0.88 0.16 0.60 0.93 0.29 0.47 1.06 0.54 0.42 Lii1 0.07 3 08-12-14 0.82 0.77 0.05 0.67 0.87 0.09 0.68 0.89 0.16 0.61 0.93 0.29 0.48 1.06 0.35 0.42 £12 0.07 4 "09-09-14 0.81 0.77 0.05 0.67 0.87 0.09 0.68 0.87 0.16 0.61 0.54 0.29 0,48 £07 0.35 0.48 L.12 0.07 5 10-07-14 0.77 0.78 0.05 0.69 0.87 0.09 0.68 0.87 0.16 0.61 0.94 0.30 0.48 1.07 0.35 0.43 1.13 0.09 8 11-04-14 0.82 0.78 0.05 0.89 0.87 0.09 0.68 0.87 0.16 0.61 0.94 0.90 0,48 L07 0.35 0,43 £13 0.06 7 12-02-14 0.74 0.78 0.04 0,69 0.87 0.05 0.69 0.87 0.16 0.61 0.94 0.30 0.AS 2.07 ,0.35 0.43 1.13 0.06 8 o1-1a;!5 0.76 0.78 0.04 0.70. "0.88 0.09 0.69 0.87 0.16 0.62 0.94 0.30 0.48 1.09 0.35 "0,43 £.13 0.05 a 01.-21-15 0.81 0.78 0.04 0.70 "" 0.87 0.08 0.69 0.88 0.18 0.82 0.55 0.30 0.49 £0O8 0.35 0.A3 £.13 0.05 10 02-03-15 0.74 0.78 0.04 0.69 0.87 0.09 0.69 0.87 0.16 0.62 0.94 0.30 0.48 1.08 0.35 0.48 L,15 0.05 11 02-03-15 0.78 0.78 0.04 0.89 0.86 0.09 0.69 0.87 0.16 0.61 0.94 0.30 0.48 1.07 0.35 0.43 LIB3 0.05 04-07 -15 0-st 0.75 0.04 0.71 - 0.88 0.09 0.65 0.58 0.16 0.62 0.95 0.50 0G4S 0.55 0.43 L.14 0.05 13 05-05-1.5 0.81 0.78 0.04 0.71 0.86 0.09 0.69 0.88 ="0.16 0.62 0.55 0.30 0.48 L08 0.355 0.43 L.13 0.05 14 06-09-15 0.82 0.78 0.04 0.71 0.86 0.09 0.89 0.88 0.18 0,62 0.95 0.30 0GAS 1.08 0.85 0.48 1.14 0.05 15 07-14-15 0.78 0.78 0.04 0.71 "0.86 0.09 0.69 0.88 0.26 0.62 0-95 0.30 0.49 Los 0.35 "0.43 £.14 0.05 16 08-04-15 0.83 0,79 0.04 0.71. 0.85 0.09 0.69 0.88 0.16 0.62 0.95 0.30 GAS9 1.08 0,35 0.43 £14 0.05 17 08-11-15 0.91 0.79 0.08 0.72 0.88 0.09 0.70 0.89 0.17 0.62 0-86 0.30 0.AS £09 0.366 0.43 1.15 0.04 18 05-1_5-15 0.81 0.79 0.03 0.75 0.86 0.10 " 0.70 0.89 0.17 0.63 0.96 0.80 0.49 1.09 0.36 0.44 £15 0.04 19 20-09-15 0.78 0.79 0.08 0.73 0.88 0.10 0.70 0.99 0.17 0.63 0.96 0.30 0.49 L.09 0.38 0.44 £15 0.04 20 10-20-15 0.72 0.79 0.04 0.72 0.88 0.09 0-70 0.88 0.17 0.62 0.98 0.30 0.A9 LOS 0.36 0.49 1.15 0.05 Note:- 7-d lCzs 7"-day28% inhibition concentration. An estimation of the concentra tion of potassiuam chloride that would oause a 25% redaction inlPirephales growth for the test population.
CF"= Central tendency (bean 1%~).
0 S = Standard deviation of the I ssvalues.
Laborato~r Control and Warning Umits Laboratory control and warning limits were established using the standard deviation of the [1%winues corrasponcin8 to the 10th and 25th percentile CVs. Tlhese ranges are t~norestringent than the control and warnieg limits recommended by USEPAfor the test method and endpoint.
5 SLio = Standard deviation corresponding to the 10dhpercentile CV. (5,A,,= 0.12) 5z= Standard deviation corresponding to the 2?t percentile CV. )SA~S = 0.21)
USEPAContmol and Warning Limits 5
a~rs=Standard deviation corresponding to the 75th percentile CV. ( *7 0.98) 5 ea= Standard deviation corresponding to the 906*percentile CV. (8 o0.45)
CVg = Coelticionr oolvariation o*tie ICrs vaiues.
USErA.250.0Ulndestandlng*and Peliutant olsdiarge Elmienslton is Whole EffluentTenidtv Appllradnns Underihe Slatinnal AzcsustinglferlMethod Variability USEnvironmental Protection Agency,Oncinnati, Program. EpA-833-R-t0-053. OH.
File: ppskntj0_*20l5.nisx CEtered by, J. Sunorer Reviewed by.
Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements SE nvironmental Testing Solutions, Inc. Organism Source: Aquatox, Inc.
1.25 - I I I I "II I I I .I I I 1 .
S1.00 2-_ 0.75
.0 b*
E 0.25
-USEPA Acceptance Criteria (>0,25 mg per surviving larvae).,
- II I I I I I I
- i I I I I I I i I I I 30*
Kentucky AccePtance Limit (< 30.0%)
(UO I-201 wW
"-2 04-a 4.' *10 w 0 aD 0
- I I I I I I I I I I I I I I I I I I I I -
30 I, III'.I I I I I I I I I -
- USEPA Upper PMSD Bound (90t percentile < 30.0%)
20 1O mj O.DD*
lO* ea ~ ~ *O **e
- P*I O O* *IO
- I I I I I I I I I I I I - I I I I I. I I 0%
Test date
--- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference*
(PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
~Central Tendency (mean Control Growth, CV, or PMSD).
SControl Limits (mean Control Growth, CV, or PMSD + 2 Standard Deviations)
Grphsgeeein~ted from associated aecelspreadalheet.
Excelspreadsheet entered by: J. Sumner
- -Page 75 of 98** Reviewed by: %.
- IT Precision of Endpoint Measurements 0
.. Pimephalespromelas EnvlronimentalTestlnysoiutiorss, Inc. Chronic Reference Toxicant Data Test number Test date Control sria Control Mean Growth cr CV CF" MSD PMSD Cr"
(%)
%) (g/lavae)
(ag/lavae), for Control Growth (mag/larvae)
(96 for Control Growth CV (%) (96) forPMSD(%)
- 1 07-08-14 100 0.631 8.3 0.06 9.0 2 08-05-14 100 0.560 0.596 9.0 8.6 0.07 13.3 11.1 3 08-12-14 100 0.694 *0.628 2.6 6.6 0.11 15.6 12.6 4 09-09-14 100 0.622 0.627 11.7 7.9 0.10 .15.7 13.4" 5 10-07-14 ioa 0.617 0.625 11.6 .8.6 0.11 17.2 14.2 6 11-04-14. 100 0.627 0.625 5.6 8.1 0.05 8.6 13.2 7 12-02-14 100 0.712 0.638 8.2 8.1 0.09 12.6 13.1 8 01-13-15 100 0.551 0.627 8.7 8.2 0.06 10.8 12.8 9 0-1-21-15 100 0.540 0.617 .10.9 8.5 0.08 14.7 13.1 02-03-15 100 0.585 0.614 13.7 9.0 0.08 13.5 13.1 11 02-03-15 100 0.607 0.613 5.5 8.7 0.07 12.2 13.0 12 04-07-15 100 0.520 0.606 4.3 '8.3 0.06 12.1 12.9 13 05-05-15 "97.5 0.536 *0.600 13.0 8.7 0.07 12.7 12.9 14 06-09-15 100 0.534 0.595 2.5 8.2 0.05 10.2 12.7 15 07-14-15 100 0.556 0.593 4.6 8.0 0.10 17.5 13.0 16 08-04-15 100 0.539 0.590 11.9 8.3 0.07 12.1 13.0 17 08-11-15 100 0.600 0.590 7.8 8.2 0.06 9.7 12.8 18 09-15-15 100 0.545. 0.588 8.7 8.3 0.06 11.1 12".7 19 10-06-15 100 0.590 *0.588 6.2 8.1. 0.07 11.7 12.6 20 10-20-15 100 0.584 0.588 10.6 8.3 0.08 13.9 12.7 Note: CV = Coefficient of variation for control growth. "
Lower CV bound determined by USEPA (1 0 thpercentile) =3.5%.
Upper CV bound determined~by USEPA (90"' percentile)= 20%
MSD = Minimum Significant Difference PMSD = Percent Mlninmum.Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatnent th~at can be declared statistically significant In a whole effiuent toxicity test.
Lower PMSD bound determined by USEPA (1 0 Th percentile) = 12%.
a'-
Upper PMSD bound determined by USEPA (90 percentile) = 30%.
CT =Central Tendency (mean Control Growth, CV, or PMSD}
USEPA. 2000. Understanding and Accounting for Method.Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH. -
.USEPA,. 2001a, 2001b. Final Report: Intedlaboretory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2 Appendix. EPA-821-R-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
-PgeEntered by: J: Sumner Pae76 of 98 Reviewed by:_ _
0 Page 1 of 6 a
Emnmo~InS~rutIonoIoo.
Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales p.romelas PpKCICR Test Number: 30_77 Dilution preparationinformation: Comments.:
KC] Stock INSS number: 1NSS ltV( _________________
Stock preparation: 50 g KCIIL:
Dissolve 50. a KCI in ILMl- water.. .____________________
Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mIL) 6 9 -- 12 15 18 21 .......
Diluent volume (niL) "994 '991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 "I0 00 Test organism information: __________ ...... Test information:
Organism age.: l*. %0*S. .. , Randomizing template: ______
Date and times organisms were 10-19-15 1600-1700 Incubator number and born between: shelf location:_______
Organism source: ATOX Batch Pp: 10{)-19-15 Artemia GUM number: CHM836 '
Drying informationfor weight
- .. determination.',______
Transfer vessel information: pH = "1PtO S.U. Date.].Time in oven:, *.iiotS 12.*
_ __ _ _ _ _ _ _ _ _ _ Temperature - "LA , °C Initial oven tem perature: 'tj ° .
Average transfer volume:' 0.1749 mL Date / Time out of oven: t..%t*
Final Oven temperature: ' C..
Dailyfeeding and renewal information:
- "Day Date Morning feeding Afternoon feeding' Test initiation, renewal," MHSW.
_____ ______________ or termination batch used
,Time Anal ,st Time Analyst Time Analyst . ............._
0 10-20-15 _______ " ,.* i,.*o 'O "so* *[ IU" 2 10-22-15 o*cK) u*[ lql * '3"': .. 1( I S2-1 6 10 15 7 10-27-15" lo~Ib~
Contrtol information:; .. . Acceptance, criteria,, Summar of test endpoints:
%M,. t ,'o1 _<20% 7-day LCso 50 ~ o*
Aver~age weight per surviving larvae: __ . >*..*
0.. mg/larvae
_.25 LeEC, ,_______
indo~.nd.nI ChV S
~o4o~o by IC2 5 ~
SOP AT21 - Exhibit AT21.1, revision 11-01-14 Page 77 of 98
Page 2 of 6 Species: Pimephales Dromelas PpKCICR Test Number: 307 Survival and Growth Data Day 0
F -
A 10 i
I -
B 10 Control I -
C 10,
~~
i D 0=
10 300 me KCI/L
~ -
F 10 I -
G I
~ -
Hi 10 0 -
U -
I Io
~
I 450 m* KCf/L J
10 K
10 L
10 10 10
- 10) '0 b0 *0 '0 b 10 I0 l0 & D i I
II
)& W i(0 10 10o (0 10 10. '0 10 to U
80 10 1010/ tO 1.0 10 10. 10 10 t0
/0 10 10 10I tO tO 10 10 tO" 10 10
/0 10 I0 101 1Q *t0 1O Tray color code:: I~l4 k*t*
A = Pan weight (nag) . '... .. ... . . .I "
Date: O~t- 2,4- I $""
B =Pan+*-La'vae weight (rag)
Analyst: 4 J/h **. *L3 *L *J*7
- V
- .1 *00 *S *P
- .*l.
Date: W'-13*
C=-Larvae Weight (mig) =B -A .
Analyst:, ,
Weight per initial number of larvae (mig)
-=C /Initial number of larvae
(¶1 Hand calculated.
Analyst... '
A, 1% £2 Average weight per initial Percent I -. 1 number of larvae (rag) ' reduction . e _
- I = = ~ 1 , =
- from control O5~ tAt
.Commentcodes: c clear, d --dead, fg tbfngus, k killed, m = missing, sk = sick, smn unusually small, ig = unusually large, dgr = deeaned and returned, w = 'wounded. .
e78 of 9 8 SOP AT2I - Exhibit AT2!.1, revision 11-01-14
0 Page 3 of 6 S
EnvJ t,,ITtIIgSdUIIWIS.IM.
Species: Pi'mephalespromelas PpKClCR Test Number: 30..7
_____ ____ ____ _ _______________Survival and Growth Data 750 mg KCIIL' 900 mg KCI/L Day . .... 600 mgKCi/L,_ __ _
_ _ _ _ _ _M N 0 P Q .R S T U V *W X 0 0 10 " 10 10 10 10 10 10 10 10 10 10
_________ __ o* to to *0 ut'
,o *
- o
- __ __ __ 10 " to Io tO \ q 4b '
a*-. *
'p0%0tItO 7 ..... \ o*i* *' .6%
A- Pan weight'(rng) -- - - - ' I Tray color code::i, I-~ 5o 5 I4 43I3i~ -
Anlst ___,_ ..
__o__.______.*2,,q,'t G.tB ,.1q i, I'~I0 15.A3 i*..*z ie.*,
D~ate: flG - .2,.-_.I ;.
B=Pan + Lairv~e Weight (rag)
Analyst: w Jb,. "* . i ,.o .,1 ,, ,,iq* i* 1" t l,. 3 ,3 1. ,S I.*
C = Larvae weight (rag) = B - A Analyst:
Weight per initial number of larvae (rag).
Hand calculated. ,. ***,t/*'
Analyst: * "* 0* *° * .* "0 Averageweight p'er iuiti"al Percent ' '
number of larvae'(mag), reduction )j*.
2.S7, OA%' 2EI&,
- Oo from control Comnment codes: c. clear, d =dead, fg = furngus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r --decanted and returned, w --wounded.
Cottments:
by VMI.yEK..n.m SOP AT21 - Exhibit AT21.1, revision 11-01-14 age 79 of 98
Page 4 of.6 Species: Pimeohales i~romelas PpKCICR Test Number: 30_.7.
I Comment codes:
Ig= unusually c =d&-r large, clear,= decanted d --dead, and fg =returned, fungus, k-=
w-=killed, m = missing, sk = sick, sm = unusually small, wounded.
b~
I,'- SOP AT21 Exhibit AT21.1, revision 11-01-14 Page 80 of 98 -
_a Pimepholespromekws Chronic Reference Toxicant Test 0
a *EPA-821-R-02-013, Method 1000.0
,I' to Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
- Environmental Testing Solutions, Inc.. Test number= 307 Test dates: Octeober20-27. 2015 Initaltnumbare, ler eyermoreS A=NawnwerhtCmii 6= Pam+-etaw lIt.. lktu~ms] W.Ile/dSwersig M ac hUten',edpt/* cooffkiaifenetvrcn r WozlutOe/lalnemharoiMa snusmleeli honwcrlgh/'l*-It MK udfileasraln Pnntnufefn Cin.in~ebtlen(nxj~l.Isj bptai.
Irm Imm Vrlll~ml AB uma~f~*CL* um~clemvme bmnms.Ntii * .~* Inun off
- zl l A 10 10 16.16 22.13 5.97 0.597 .0.367
- 6 " 10 10 16.57 21.50 4.93 0493 0.8 060.461100 0541.6 Ntapial Control C ... 10 " 10 15.94 22.16 £352 0.632 - .63J2 100 0591. oeola
- E 10 10 16.55 22.29 5.74 0.--/4 0.574 P0 10 10 16.72 22,45 .5.73 0.573 "0.57310. 0.29288 0 10 10 16.41 21.21 4.80 0.460 - 0.460
- _______ II 1 10 15.07 20.03 5.01 0.501 ________ 0.501 __________ ______
I 10 10 16.14 21.05 4.91 0410.491 450 J 10 10 - 15,49 20.86 5.37 0.537 0.537 100 094701.
C :10 10 16.24 21.76 3.52 0.552 .4700,552
- L 10 10 £4.81 19.56 4.77. 0.477 ________ 0,47-7 _____ ______
M 10 9 15.09 19.39 4.30 0AT67. 0.430 60NP 1o 9 16.86 2.1.62 4.76 0.529 0466 10.7 0.476 9. .6 062.
60 o . o 0 15.52 21.22 5.30 0.530 "0.510 6. .6 062.
P 1 10 15.42 19.62 4.20 0.4251 0A420o ____ ____________
O 10 9 14.43 19.4.5 5.02 0.556 0.502 750 5 .. 10 B 15.43 19.42 1.99 0.496 0.39615259.3 1. 2.
S . 10 7 13.91 17.91 4.00 0.571 0,400
_____"__ 10 9 14.42 15.66 4.24, 0.471 -0.424 *______ ______
U 10 7 1450 18.33 -3.43 0.490 0"343 W 10 6 16.20 18.59 3..39 0.565 - 036 - 650.335 50 030554.
X "10 7 15.55 19.72 3.77 03519 . .377 _ ___ ______ ______
1' 10 3 16.18 17.60 1.42 0.473 0.142 120 10, .. 1 16.57 17.41 1.04 1.040 ,7 570.104 20.0 0.129 14.5 78.0 LA 10 2 16.06 " 17.32 1.24 0.620 0..1.133.7
_________ 6 10 2 16.22 1i7.66 1.44 0.720 _______ _______ 0.144 _____________ ________
Dunneetu' MSOvalue: 0.0811 MIVD'= Minimum S]gn~fcant Difference P635D: 13.6 PMSrO = Percent Minintum Significant Difference PMSD is a measu;re of lest p recision. The PN145 isthe minimum percent dlffereeee between the esetrol an troaltmentlthat can be deciared statistically significant In a whole effluent tosicity teet.
Lower PMSD bouuid determined by USEPA (10th percentile) m12%.
Upper PMlSDbound determined by UIIPA (90th perceetite) =3014.
iLower and upper PMSD bounds were determined from the +/-0th 3rd 60th pe rcentlie, mapectively. of PMSD0data from EPA's WET ieterln boralory VeriahilityStudy (USOPA, 2501,5; UIEPA, 2001b).
USEI'A. 200oa, 2001b. Finel Report: Interleber'utory Variebilityltudy of EPASIhort-terrnvChronlc and Acute Whole Effhuent Tonicity Test Methods, Volumes leand 2-Appendie. EPA-821-6-01-.004 and EPA-821-5-01-O05. US Environmental Proteetion Ageecy. Clnclinnati, OH.
P*ile: ppicci-tr 0Z01S.clsx Reviewed by: *f
tonmoJt*IThatn~SaIw1on,,
I~.
Lerval Fish Growth and Survival Test-7 Dae Survival Start Date: 1O/2012015 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 10/27/2015 Lab ID: ETS-Envir. Testing Sal. Sample Type: KCL-Potassium chiloide Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephsles prameias Comments:
Conc..mglL 1 2 3 4
- D.Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000. 1.0000 480 1.0000 1.0000 1.0000 1.0000 600 0.9000 0.9000 1.0000 1.0000 750 0.90003 0.8000 0.7000 0.9000 900 0.7000 0.6000 0.6000 0.7000 1050 0.3000 0.1000 0.2000 0.2000 Transform: Arasin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-.Mean Mean Mmn Max CV% N Sum Critical Reap Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 "0 40
.300 1.0000 1.0000 1.4,120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 460 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9500 0.6500 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 40
- 750 0.8260 0.8280 1.1491 0.9912 1.2490 10.866 4 10.00 10.00 7 40 "900 0.6500 0.6500 0.9356 0.8861 0.9912 6.464 4 10.00 10.00 14 40 "1050 0.2000 0.2000 0.4572 0.3218 0.5796 23.087- 4 10.00 10.00 32 40 Auxiliary Teats Statistic Critical Skew. Kurt Shapiro-WIli's Test indicates nlorrnaldistribution (p > 0.01) 0.91641. 0.869 -0.369 0.51366 Equality of variance cannot be confirmed Hypothesis Teat (1-tell, 0.08) NOEC LOEC ChV TU Steels Many-One Rank Test 600 7850 670.82 Treatments vs D-Control Maximum Llkellhoodi-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical *P-value Mu . Sigma iter Slope 10.3756 1.56043 7.31719 13.4341 0 3.9497 9.48773 0.41286 2.96401 0.09638 3 Intercept -25.753 4.58766 -34.745 -16.762 TSCR 1.0-Point Probits mgIL 95% Fiducial Limits 0./
ECO1 2.674 549.286 449.219 615.4890.
EC06 3.386 638.971 554.374 694.609 o.8 EC10 3.718 692.621 619.065 742.166 0.7 EC15 3.964 731.342 668.988 777.169 EC20 EC25 EC40 4.158. 763.653 704.855 607.246 4.326 792.509 738.968 838161 4.747 870.15 824.957 918.041 ap0.6 o.s1 04 EC50 5.000 920.474 874.807 979.405 04 .
- EC60 5.253 973.709 922.5 1050.25 0.3 '
EC76 5.674 1089.1 1000.91 1167.850.
ECS0 5.842 1109.5 1032.4 1249.090.
EC85 6.038 1156.52 1069.72 1326.26 0.1 ECO0 6.282 1223.28 1117.88 1428.62 0.0 EC95 6.645 1326 1192.28 1598.19 1 10 100 "1000 180000 EC99 7.326 1542.8 1343.42 1978.43 Dose mglL Dose-Response Plot 0.9i 0.81 o.61
- 0.4i 0.2' 0.1i Filc ppkclcr_102015.sxl Entel:redby: J. Sh*mern Reviewed by: __*s~
82 of 98
9 0
0 C
Statistical Analyses tnvtronm.isniintieasetnntitte.
Larval Fish Growth end Survival Test-7 Day Growth Sample ID: REF-Ref Toxicant Start Date:
End Date: 1012012015 10J27/2015 Test ID: PpKCICR Lab ID: ETS-Envi rt. Testing Sot. Sample Type: KCL-P~otahsium chloride Sample Date: Protocol: FVVCHR-i SPA-521-R-02-013 Test Species: PP-Pimephalea pronmelas Comments:
Conc-mgIL 1 2 3 4 D-Control 0.5970 0.4930 0.6320 0.6120 300 0.5740 0.5730 0.4800 0.5010 450 0.4910 0.5370 0.5520 0.4770 600 0,4300 0,4760 0.5300 0.4200 750 0.5020 0.3990 0.4000 0.4240 900 0.3430 0.3350 0.3390 .0.3770 1050 0.1420 0.1040. 0.1t240 0.1440 I-Tailed Isotonic Conc-mgJL Mean N-Mean Moan ... Transform:
Min... Max Untransformed CV% N t.Stat Critical MWD Mean N-Mean D-Control 0.5835 1.0000 0.5835 0.4930 0.6320 10.628 4 0.5835 1.0000 300 0.5320 0.9117 0).5320 0.4800 0.5740 9.151 4 1.464 '2.290 0.0811 0.5320 0.91 17 450 1.0.5143 0.8813 0.5143 0.4770 0.5520 6.986 4 1.955 2.290 0.0611 0.5143 0.8813
- 600 0.4640 0.7952 0.4640 0.4200 0.5300 10.842 4 S3.374 2.290 0.0811 0.4640 .0.7952 750 0.4313 0.7391 0.4313 0.3990 0.5020 11.281 4 0.4313 0.7391 900 0.3485 0.5973 0.3485 0.3350 0.3770 5.532 4 0.3485 0.5973 1050 0.1285 0.2202 0.1285 . 0.1040 0.1440 14.510 4
- 0.1285 0.2202 Auxiliary Tests . .Statistic Critical Skew Kurt Shepiro-Wilk's Test indicates normel distribution (p >' 0.01) 0.93919 0.84.4 -0.3921 -0.8388 Bartlett's Test indicates equal variances (p = 0.88), 0.75495 11.3449
,H.y~pothesis Test (1-tail, 0.08) NOEC LONG ChiV . TU MSDu MSDp 1535 155 F-Prob .df Ounnati's Test 450 600 519.615 0.06111 0.13901 0.00973 0.00251 0.0377 3, 12.
Treatments vs D-Control. . . ... , .
Point malL SD 95% CLIExp,) Skew ICo5° 169.95 115.20 .31.34 848.89 0.9788 10[10 357.89 123.81 51.89 702.17 0.2743 0C15 804.55 109.99 97.20 782.28 -0.2747 1.0o 1C20 591.64 92.60 3990.18 "568.07 0.4122 .
1025 720.80 53.65 453.08 870.42 -0.3573 a 1040 827.10 23.73 802.35 939.69 -0.5913. 0.8 1C50 938.89 10.17 909.28 970.18 0.0687 .
- indicates IC estimate less than "thelowest concentration*
0.4 0.3 0.2 0.1 0.0 , ,
500 1000 1590 Dose mgIL Dose-Response Plot 1-tail, 0.05 level of significance 1 0.4' C.3.
0.2 IDdQtfldSflt enliw by Kelleyt.K*eeae:
Pile: ppklcdrj102OIS Enteredby: .xlsx 5.Spiner Reviewed by:.~I Page 83 of 98
Page 5 of 6 nwenxaITe,,gsor~IoniI~,,.
Species: Pimephalesvromelas PpKC1CR Test Number: 307 Daily Chemistry:
Day (Analyst identified for each d' '.-~---------~---~--------;~
nerfbrmed nl-LD.O, and conductivity measurements only.
0 2 Analyst !i) A/ iI~A3 ~& IISi~ I~u.) II ft/&
Concentration Parameter i
pH (S.U.) ~oo If-~qq-
- IIF *7.1l~rI-l~c~ H 70 q6
~sJL~I
]DO (mag/L) 7,'
Conductivity 30 32g.
CONTROL
- Hardness (mg CaCOsL) _____
~o.% ~¶
_ __ ___ ~
IV*. ............... '- . 6*.ij _. II o I ,1WI !I *
- pHo(S..) __,i ____ __ .___ , . 17 45gK IL Conductivity " ___"
______ *Temperature (%-C) "RA, * "1A[*. -*q*i~A "*,0 ' "'ll 400 mig KCIIL Conductivity "
0
.......... *Tremperature ( C) -- '- . = ",A~ ' "L, .1 "iO 600 mg KCI/L Conductivity Llf2O- *** l_
DO(m*L) . , 14*' 77".'
900 mg KCI/L Conductivity l O*" [ *O
........ __.. ..... _ *Temnperat~ur e(;oC). 1AO*, *1$ ll _S1.. ._.1A. . ...
pH(&.u.) ,71(*
DO(rag/L). 17,' -I '~~-
1050 mg KCIIL [Conductivity _, -- /-
~~AiO
- 'Temperature (00) a Finamfil Initial Initial
- *Temperatures performedperformed nity and hardness at the time byofthetest initiation, analyst renewal identified or termination on the bench sheetbyspecific the analyst identified for each analy~sisinand the Daily Renewal transcribed Information to this tableby:located@og Page 1.
bench sheet
Page 84 of9 SO A 2 -Ehii "TIleiin1-11
Page 6of6 Gdr.n~ntaJTeitIn;S.ri.t!o.s.!ne.
Species: Pimevhkales promelas PpKC1CR Test Number: 307_
(Anlvsidntile fo**Day (Anlys Jdntiiedforeach day. i~erformied pH, fl ity measurements only.)
6 AnalystF ' t*,
k cb*
Concentration Parameter pH (S.U.)
DO (mgIL)
Conductivity
(~axnhoskm)
CONTROL *Mkahinhiy (mg CaCO3/L)
- Ijt~rdn~
(mg CaCO 3/L)
- Temperature a.(0C) pH (s.u.) - *'2 I-DO(rng/L) 300 mg KCI/L Conductivity
(~+/-mhos/cni)
- Temperature (0 C) 450mg KCI/L Conductivity *0 *il' ' l *
- l7., r l DO(mg/) 7.7 h7,i z 600 mg KCI/L Conductivity 75mg(SL .nU.)vi *7 16I 3o 1/ . -
750 mg KCIIL Conductivity
- 2 0 * "g (uimhoscm) 9050 mg KCI/L Conductivit y*o **
DOTemperature initial Final Initial *inai Initial *nal Iaitial Final E.;ve ratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located tin Page 1.
A1¢l/ity Ai I and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: _.**
\ Y" / SOP AT2I - Exhibit AT21 .1,revision 11-*01 -14 Page 85 of 98
Ceriodaphniadubia Chronic Reference Toxicant Control Chart 4 Environmental Testing Solutions, Inc.
J I I I I 1.14 "USI* i I I I I J I I I I I I I I I
- PA Control Limits (IL2 StandardDeviations) 1-.12 1.10 1.08
- 1.06-boe~~o.........................*.....,..***t***~b**
1.04 ............ ................ ooem 1.02 1 I I I I I I I I I I I I I I I i I I 2.5 , I I I I ,. ,I I I Ii 1 i I I I I S 2. USEPA Wantning and Control Limits (75t and90t PercentileCl's)
,, 1.5 e l peo oo e el Q 1.0 . S S S 0.5 .. *f9*................
I
- I* , 1 I I I I I I I I I i I .1 I I l
. . . . . I 1 I 1 I 1 1.4 I I I I t i I i I I I I I I I I j j J I Laboratory*Warning and ControlLimits (J 0 th and 2 5 tA Percentile CVs) "
1.3 1.2 1.1 1.0 0.9 Air I I I I 1 I I I I I I i I I I i i i I I
- Test date
-.- 7-day lC 25 = 25% inhibition concentration; An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- . Central Tendency (mean IC2 s)
--- Warning Limits (mean IC25 + SA10 or SA.TS)
CnrlLmt(maIC5 + S 5 .0. 9O or Stndr Deiaions).
Gruphsg[erwrated fromeuroclnted excels*pr*,ads heet,
- Page 86 of 98 En.Ispr.dsh..t entered by:JSme Rmv~uwud y J u. .
Co Ceriodaphniadubia o* .. Chronic Reference Toxicant Control Chart CO Environmental Testing Solutions, Inc.
State and USKPA Laboratory USEPA USEPA 1-alsoratory Testnumiser Tesedate "7-dayiC *3 CT S Control Limits Se.so Warning Limits S* Control Limits S,.T Warning Limits S* Control Lmits CV (g/t NaCI) (8/INaeI) CT-2S CT+25 cT-s, a*L'T÷s,.w CT -S5un C-'C÷ u.n aT- SA,* aT ÷ SJLT T*a.;5 Cr-÷ S~J0 04-08-14 1.05 1.06 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.88 .1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 2.
05-05-14 1.05 "1.05 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.68 1.24 . 0.4.8 0.58 1.54 0.56 0.40 1.72 0.01 3 06-03-114 1.05 1.05 0.01 1.04 . 1.08 0,06 0.98 1.15 0.18 0.88 1.24 0.48 0.58. 1.84 0.86 0.40 1.72 0.03.
4 07-08-114 1.07 1.06 0.01 1.04 1.08 0,08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 ' 0.88 0.40 1.72 0.01 S 08-05-14 1.06 1.05 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.88 1.24 0.46 0.58 1.54 0.65 0.40 1.72 0.01..
6 09-09-14 1.07 1.08 0.01 1.04 1.08 0.08 0.98 1.15 0.18 " 0.88 114 0.48 0.58 1.54 0.66 0.40 1.72 0.01 7 10-07-14 1.08 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.16 0.88 1.24 0A48 0.58 1.54 0.66 0.40 1.72 0.0+/-
8 10-07-14 1.00 1.06 0.01. 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1124 0.48 0.58 1.54 0.66 0.40 1.7"2 0.0"1 9 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.58 0.40 1.72 0.01 10 12-02-14 1.08 1.06 0.01. 1.04* 1.08 0.09 "0.98 2.15 0.18 0.88 1.24 " 0.48 0.58 1.54 0.65 0.40 1.72 0.01 11 01-23-15 ,1.06 1.06 0.01 1.04 . 1.08 .0.09 0.98 1.1.5 0.18 0.8"8 1.24 0.48 0.58 1.54 " 0.68 0.40 1.72 0.01 12 02-03-1.5. 1.07 1.06 0.01 1.04 1.08 0.09 0.98 :1.5 .0.1.8 0.88 1.24 0.A8 0,59 1.54 0.55 GAO0 1.72 0,01 13 03-01-15 1.06 1.06 . 0.01 1.04 1.08 0.09 0.99 1.15 0.18 0.88 1.24 0.46 0.59 1.54 0.68 0.40 1.72 0.01 14 04-07-15 1.06 1.05i 0.01 1.04 1.08 0.09 0.98 1.15 0.16 0.88 1.24 0.48 0.80 1.54 0.88 0.40 1.72 0.01 15 05-05-15 1.07 1.05 0.01 1.05 1.08 0.09 0.98 1.135 0.18 0.88 .125 OAK8 0.59 1.54 0.65 0.40 1.72 0.01 16 06-09-15 1.08 1.06 0.01 1.04 1.08 0.09 0.90 1.15 0.10 0.88 1.25 0.48 0.59 1:.54 0.66 0.40 1.72 0.01 17 07-14-15 1.06 1.07 0.o1 2.05 1.08 0.08 0.98 '115 .0.19 "0.88 1.2.5 0O48 0.59 1.54 0.66 0.40 1.73 .0.01 18 08-04-15 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.4B 0.58 1.55 0.58 0.40 1.73 0.01 19 09-15-15 1.07 1.07 0,01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.56 0.41 1.73 0.01 20 10-06-15 1.08 1.07 0.01 1.05 1.08 0.09 0.08 1.1`5 0.18 0.88 1.25 0.45 0.59 1.54 0.55 0.40 1.73 0.01 Note: 7-d ic* = 7-day 255%inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Cerionlophnio reproduction for the test population.
CT Central tendency (mean 1Cm)..
S =Standard deviation of the lC.vs vlues.
Lab oratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the iC* values corresponding to the 10th and 25th percentile Ct/n. 'These ranges are more strngent than the control and warning limits rtcom~nended by USEPA for the test method and endpoint.
-* Standard deviation correspondingto the i10' percentile CV. (5z 0.0B)
Sz = Standard deviation corresponding to the 25e" percentile CV'.(S~z = 0.17)
UJSKPA Conical and wamning Limits S**= Standard deviation corresponding to the 75" percentile CV. (Sa = 0.45)
$un = Standard deviation corresponding to the9Oe percentile CV. (5S~ = 0.52)
CV = Coeffitient of variation of ithelIwvalues.
USEPA. 2000. Understanding and Accounting for Method Variabli1ty In Whole Effluent Toxicity Applications Undor the National Pollutant Discharge Elimination Program. EPA-833-lt-00-003. US Environmental Protection Agency, Cincinnati, Cl-.
FRe: CdNa~CIt_100l5.nisx Entered by: J. Sumner Reviewed hy.
4"Enromnl I Ceriodaphniadubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 - I I I I I "I I II I I I I I I I I
- I I -
35 0
30 0
25 I-0 20 0
0 Q 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
- I I I I 1 I I I 1 I I I 1 1 I I -
I I I I i I I I I. I I I I I .I I I I I I I S40* North Carolina Acceptance Limit (< 40.0%)]
Kentucky Acceptance Limit (<30.0%)
c* 20 50 E
- II I I I I I I I I I I I I I I 1I " I 40 K USEPA Upper PMSD Bound (90t percentile < 47.0%)*
0 30 20 10 I I.I.."....I..I I I I I'* I* I I I .I. I "
"I
- 4~W 8
~A ' ~9' ~~.Xah~1AN XIrX 5 4~V~ ~ ~ ~ ~ ~6-~
Test date
--- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
-- -. Central Tendency (mean Control Reproduction, CV, or PMSD)
.** Control Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)
Gi~phs generated flora augcl.ted eac.I.pre.dshe.t.
- Page 88 of 98 "" toed ,preadsheet enteredby: J. Sumner Renlawedby:
Precision of Endpoint Measurements Ceriodaphniadubia Chronic Reference Toxicant Data SEnvironmental Testing Solutions, Inc.
Testnubr Test date Control SControl Mean Survival Reproduction. Cr CV a~ MSD PMSD a1 for Control Mean for Control Reproduction (%5) .Reproduction (95) PMSD (%)]
(95) {offsprlngffemale) .for (offspring/female) CV (95) 1 04.08-14 32.4 6.7 2.1 6.5 2 05-06-14 1O0 31.3 31.9 5.2 6.0 .1.7 5.5 6.0 3 06-03-14 100 34.6 32.8 3.9 5.3 2.3 6.6 6.2 4 07-08-14 1 00 34.5 33.2 3.1. 4.7 1.7 4.9 5.9 5 08-05-14 100 34.0 33.4 5.0 4.8 2.3 6.8 6.1.
6 09-09-14 100 32.6 .33,2 4.6 4.8 2.0 6.1 6.1 10-07-14 100 29.7 32.7 4,8 4.8 2.2 7.4 6.3
- 8. 10-07-14 100 32.1 32.7 3.1 4.6 2.1 '6.6 6.3 9 11-04-14 100 29.8 32.3 5.0 4.6 1.8 6.2 6.3 10 12-02-14 100 33.1 *32.4 6.4 4.8* 2.0 6.2 6.3 11* 01-13-15 100 32.7 32.4 3.8 4.7 1.8 5.6 6.2 12 02-03-15 100 32.5 32.4 3.9 4.6 1.8 5.6 6.2 13 03-03-15 100 32A 32.4 6.0 4.7 1.9 5.8 6.1 14 04-07-15 100 32.3 32.4 4.6 4.7 1.8 5.5 6.1 15 05-05-15 100 31.7 32.4 5;.6 4.8 1.8 5.8 6.1 16 06-09-15 100 29.7 32.2 2.8 4.7 1.4 4.8 6.0' 17 07-14-15 100 30.8 32.1 3.0 46,6 1.5 4.8 5.9 18 08-04-15 100 31.0 32.1 3.0 .4.5 1.6 5,0* 5.9 19 09-1.5-15 1O00 29.4 31.9 4.0 4.5 1.7 5.9 5.9 20 10-06-15 100 29.6 31.8 4.8 4.5 S 1.8 6.1 5.9 Note: CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (l10lpercentlle) = 8.9%.
Upper CV bound determined by USEPA ( 50 thpercentile)= 42%
MSD = Minimum Significant Difference PMSV = Percent Minimum Significant Difference PMSD is a measure of test preci~.ion. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a *vihoie effluent toxicity test.
Lower PMSD bound determined by USEPA (2 0 t percentile) = 13%.
Upper PMSD bound determined by USEPA (90t percentile) = 47%.
aT = Central Tendancy (Mean Control Reproduction, CV, or PMSD).
USEPA. 2000. Understanding and Accounting for Method Variability in Whale Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPAo833-R-0D-003. US Environmental Protection Agency, Clncinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-D-01-004 and EPA-821-B-01-005. US Environmental Protect~on Agency, Cincinnati, OH.
"*' .... File: CdNaCICR._1006]5.xlsx Entered by:, J, Sumner Page 89 of 98 R*ewby;__
Page 1 of 6
.1 E~oI~do.od~.
Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodayhnia dubia CdNaCICR #: 17..99 Dilution preparation info'rmation: ,.... Comments:
NaCI Stock INSS number: INSS Iflbl __-___.........._______
.Stock preparation: 100 g NaCI1L:"
Dissolve 50 g NaC1 in500 nmL Milli-Q water.__________________
..D~iuton prep m*)'1 600 800 1000 1200 1400 .......
St1 oue(L 2 .I 15 . 18, , 21 _ _ _ _ _ _ _ _ _ _ _ _ _
DilentvolumeQ(m) 491 1488 1485 1482 1479 ,- . ._______________
Ttloue(L ... 50, 1500 1500(J1500 1500 _________ _________
Test organism source in ormaaion: Test information:.
Or anism ae: < 24-hours old Randomizing* template color:
Date and times organisms were born Gto~-Ce 0 't b tL. Incubator number and shrif between: . . .. location: 2*
Culture board: -* t**
Replicatenumber: I 2 [3 [4 ]S ]6 [7 8 9 10 YWTbatch:
Cultureboardeupnurnber: Ia I ]iiq It I Z*ItIk[lo~*0"'
0 Transfer vessel information: H -- ,o. _SM.. Ternperture = -- *,*C Selenastrum batch:
- Average transfer volume (mL): 0.012 1 joL .. 0*q° Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used
-_ termination time
.0O1-06-15 . ___ "_____._.____ o:',.,,
1* 1 10-07-15 .______________. (*t 1 eot "*
2 10-08-15 _____________ *o'.0V¢s
__ __ 10.09-1S ..
________________ t-to- a
____ 0-11-15 e3._o*O *,"O-r h )
6 10-12-15 SControlinformation: _ ___ Acceptance criteria -Summary of test endpoints:
% of Male Adults:
%Adults having 3 rd Broods:
%Mortality:
. 0~1 ,<20%
O1. ,.<20%
. 80%
7-day LCs0 NOEC LOEC J "7 1
.,t2 cO _
I*
I Mean Offspring/Female: 2A.,* h 15.0 offspring/female ChVY j t1( .S Indepndet
~Anw by age 90 of 98 SOP AT14 - Exhibit ATI4.1, revision 1 1-01-14
D 0
Page 2 of 6 a
-,J Species: Ceriodaphaniadubia CdNaCICR #:. 17_.9 CONTROL Survival and ReproductionData
_ __ _* - Replicate number . __
Day___ 1 2 3 4 5 [6 7 8 .9110
[Adult mortality k. .J_ . 1- ~
- I...
2f] Young poduced *, I
..... >... .CL..O .C1. 0 I I I__
(J(J *:1 I Youngprod,,ced Adult mortality f_ [ ___ f1 Qi j
!.I... [...
Q) 4 I YoungprodUCed 1W1I.. ~I A..~I~I-
____ I Young produced ~~LV'~ O %L i 6[Adult mortality \. ___Y___ngproduced__
Total young produced " *I -.. I 7.* -. I7,"?. ,* I 1.. I . V'* 3o Final AduitMortalit \.- I W I .
's -. 4.. " t:2 -- :-* t.. c-- '...
Note: Adult mortality (L =f live. D = deadil. 51B= s ro ml nlit ro ot between two daysi. CO = carry over (offhorinc carried over with adult during transfer). ...
II Ill I I Concentration: I.
~%Mortality; I (37 I
-Replicate niumber Day 21 $ 6 7 8 9 10-C:
1 Young produced Adult mortality 010
- . *.J 0..1 010O c~ioic~ic 21 Young producedIl Adult mortality cflL.- ]* k.[I .. J*
I L_[j* *L J I....] I (3-1 L,.j <J L.,- I L-- c -,..
I" 3 _Youngproduced i *1JO CL I 0O A1 fl cl I J
.~I Adult mortality *' JL L.* 1'*--I 1 *" I*-1 "-- I_ I" I-"'
4 Youngproduced '-. " k.I*_ .. *** .. [*_. * [. ,
SAdult mortality J '...\..__ .- J
'."J_ L"~ ' - L.
[.Young produeed I. Lt. k** [,I If _____ ] ____ _ ___lL
'Adult mortality ~ IC"
- j- L. ' I *.. [,,- ':~
Adl ...m ralt * . ._U.,,__ -- [ . [ *.
Young produced. 0$ II i* l .1C 0 0 IA * '*
Total young produced "* * "I_'* *- "*-Z *L *( *(o *'% -
Final Adult Mortality * *. ... *...
i**. -.. cr
- caridedAdult
- Note: mortality over with (L =live, adult during D = dead), SB = split brood (single brood spilit between .two days), CO =carry over (oftspring transfer).
I%Concen~tration:
Mortality:
Mean Offspring/Female: I 0.
¢07 IadQ.~d.nt
% Reduction from Control: - ***
Misy E. ~
SOP AT14 - Exhbit ATI4.1, revision 11-01-1 4 LA..-
Page 91 of 98
Page 3 of 6 Species: Cerlodaplhnia dubia CdNaCICR #: 17.99 80 ~NaCIIL Survival and Reproduction Data
__a _ __ 1112 T ____ _,,,
3 4 Replicate number 5 6 7 8 .9 10 2 Young produced- C) T2 (t 01 D 0 o.O 1 Adultimortality "- 1 Z . '- - '-I . -I'-
2 [. Young produced-1.c. __
Adultgportality Jl 'C I '-I C- I '- I s-'aI_'0 I50 ___
Adult mortlitY j k_ *--- U:- .,-.J_U U * -A 1 '-1'- __-
61I Young produced JCj I i I )I I--1 0 ) 0
-I Note- Adult mortality (L = live, D =*dead), SBS - split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). ____________________
IConcentration:
0 AMortlity: 17.
SMean Offspring/'Femnale: I 3. t*
I%Reduction from Survival andReproduction Data Control:" "-5.'1 ,I, 1000 m~NaCIIL
[ 1
____________ 5 number 6Replicate 8 9 1 Day__ 2_ 10617 8 9 1 Young produced * £ _0 0*
- 2 ~Young produced ] 9 0 t "-0 Adult mortality ~ ~ _ __ _ _
4 Young prodUced __ _ __ _...o __
S mortlity YunpducAdult ___ __ __ j, L I.
- .[,
4oa young produced 1 1 II2k 1U~ !~j ialAdult Mortality j ..,T pL,_, , _ L * .q__._ ___
ote: ( lieJ-Aduit mortality edS ' -slibod(ine broo splt bewe[ \4_ tw. das) 'O -I--Icryoe (ofp carried over with adult during transfer).
Concentration.
h.do~.,d7nt Mean Offspring/Female:
- 1_2 R..i.,, hy Reduction fro.m Control: I Y.II.yLX.rnfl
-°4 7. S%
SOP AT14 - Exhibit ATI4. 1, revision 11-01-14 Page 92 of 98
Page 4.of 6 Species: Ceriodaphniadubki CdNaCICR #: 1 7_99 i1200 m ~NaCIl* Survival and Reproduction Data
........... Replicate number Day ______ 1i 2,3 14*___ I5 6 718 9
1 I!~Young produced Adult mortality L. *...__'
01~
I" .. '-_ .. '-
O~
I.__-I *..s-'
II Young produced 01 10 : . OL i0
- 4- ".
- i
__ Adult mortality _ ~P '
Adult mortality '-__If-____* -.
Young produced O~_~~ * " IiILA -OL J Adultmortality*[
- lI 6 I 6[YoungAdlmrat produced dl otliy Yon prxod.,uced j
I 4
f* ]
5 1."'4 I.-
L...o...
- ).
L. .j--
0
.ŽL I
£
....o..'.
el Total young produced [* * * .* *
% l*
"*g ]* t t%
Final Adult Mortallt ' ..- %-.- %. %-- %.: '-- %.--' -
Note: Adult mortality (1.= live, D = dead), SB =split brood (single brood split between two days), CO = carry over (offspring carried over with adult daring transfer)._____________________
IConcentration:
-%oMortali: 1O' IMean Offspring/Female: I
- I
% Reduction from Control: IA.tu 1400 mg NaCIIL Survival andReproduction Data
_______ ___Replicate number ____.
Day ________, 1 2 1 3 1 7, 8 9 110
___j .Adult moilalit L_. * .. L.*
=. _ I _ 1 - I-2I Adult mortalityj j.~~ - L ~ t~-I. L.
.6 IYoung produced Adult mortaliy ",*
_ _ j)
°'_ _ _
I Young producedJ(* (.* I* . t0 C)I J " L ( I:)
T ~otalong prodluced
- 0 L C) 101 : ")" C) ,0 O *.'JL
_Fna Adult Mortality %.. ______ __I'.. _'-I'.- I_'
irene: ImorflUIIy tt. = liVe, J.I =ogaU), *fl = IpilI e~rUoUIS1ngIO OIoUU *pZI oOIWCCJn IWOueys), LU = mrly over moyu~pnim carried P.UUlt over with adult during transfer).__________________
S IConcentration:
flMean Offspring/Femal'e: I2~0
% Reduction from Control: **'T' SOP AT14 - Exhibit ATi4:I, revision 11-01-14 93 of 98
Verification of Ceriodaphnia Reproduction Totals 0
- Environmental Testing Solutions, Inc.
CO co Control 1000 mg NaCIiL-
- Replicate numberToa Day 1 2 3 "4 Rep)icate numberToa 5 6 7 8 9 10 Tta Day ~1 2 3 4 5 6 '7 8 9 10 Tta 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 00 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0* 0~ 0 0 3 0 0 0 0 0 0 0o 0 0 0: 0 4 -" 4 3 4 3 3 3 4 3 5 3 35 4 4 5 3 4 5 4 5 3 3 3. 39
- 5 10 12 12 9 10 12 12 11 9 1I 108 5 12 10 11 11 9 10 10 10 11 10 104 6 0 .0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0
.7 15-13 13 16 16 15 16 18 151I6 153 7 15 18 15 13 15 12 15 14 14 15 146 Toa 29 2 29j28 29 30 32 32 29 30 296 Total 31 33 29 28 29 26 30 27. 28 ,28 289 600 mgNaCFlL 1200 mgNaCI/L_______
liReplcate numberToa Day Relct number ,- - Total Day 1j 2~ 3 4 5." 6 7 8 9'
- I Tta '1~-* 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0. 0 0 0 2 0 a 0 0 0 0 0 a0 0 _ 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0.0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4. 3 3 4 4 4 4 4 3 4 37 4, 4 2 28 5 12 10 12 11 13 12 101I012 10 112 5 0 0 3 0 0 54 0 0 3 15 6 0 0 0 0 0 0 0 0 0 0 0 *6 7 8 .0 5 40 0 74 0 35 7 15 17 14 17 15 16 16 16 18 14 158 7 0 0 4 0 0 3 5 0 0 0 12
- - - - - - a - - - -i a..........7 Total 31 30 29 32 32 32 30 30 33 28 307 Total 9 11 11 7 7 11 13 8 8 5 90 800 mgNaCIIL 1400 mg NaCI/L Da Re *licate numberToa Da - Re lieate nuberTotal Dy 1 2 3 4 5 *6 7 8 9 10 Tta Dy 1 2 3 4 5 6 7 8 9 10
- 0 0 0 -0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 .1 0 0 0 0 0 0 0 0 0 0 0 2 0.0 0 0 0 0 0 0 0 0 0 2 0 a 0 0 0 0 0 0 0 0 0 3 0 0 _0 0 0 0 0 0 0 0 0 4 4 5 4 4 3 4 4 4 4 4 40 4 2 0 1 3 2 1 1 2 1 2 15 5 11 -12 12 10 11 11 11 13 12 12 115 5 0 2 0 0 0 0 0 0. 0 0 2 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 16 17 13 1.5 15 16 13 16 13 151 7 0 0 1 0 0 0 2 0 0 0 3
-I- - II- - - . ** *. *-
- a *. . - . I Total 32 33 33 27 29 30 31 30 32 29 306 Total 2 2 2 3 2 1- 3 2 1 2 .20 File: CdNaCICR_10061 5.xlsx Entered by: J. Sumner Reviewed by:
to O'*P
- r]f*"Ceriodaphnia
- i:EPA-821-R-02-013, dabia Chronic Reference Toxicant.Test Method 1002.0
- EwlrnmetalTesingSolutions, Inc. Yeiiaion of Dat Entry, Calculations, and Sta~tistica Analyses Test number: CdNaCICR #179 Test dates: October 06-13, 2015 Concentration _____Replicatre number _ __ ___ Survival Average reprodnction Coefficien of Percent rmlaetii from (rng/L NaCG) 1 2 3 4 5 6 7 8 9 10 (%) (offspringlfemale) VSriTIZIO (55) cootrol (°"A)
Control 29 28 29 28 29 30 32 32 29 30 100 29.6 4.8 Not applicable 600 31 30 29 32 32 32 30 30 33 28 100 30.7 5.1 -3.7 800 32 33 33 27 29 30 *31 30 32 29 100 30.6 6.4 -3.4 1000 31 33 29 28 29 26 30 27 28 28 100 28.9 7.0 2.4 1200 9 ii 11 7 7 11 13 8 8 5 100 9.0 27.2 69.6 1400" 2 2 2 3 2 1 3 2 1 2 100 2.0 33.3 93.2 Bennett's MSJD value: 1.813- MSD ='Minimum Significant Difference PMSD: 6.1 PMSD = Percent Minimum Significant Difierence PMSD is a measure oftest precision. The PMSD is the minimum percent difference between'the control and treatment that can be declared statistically significant Ena whole effluent toxicity test Lower PMSD bound determined by USEPA (10"' percentile) = 13%.
Upper PNISD bound determined by USEPA (90"' percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th petcen/tile, respectively, of PMSD data from EPA's WET Interiaboratory Variability Study (USEPA, 2001 a; USEPA, 200 ib).
USEPA. 2001a,.2001b. Final Report: lnterlaboratory Variabiliy Study of EPA Short-term.Cbronic and Acute Whale Effluent Toxicity Test Methods, Volumes I and 2-Appendix. 2PA-421-B-01-004 and EPA-821-B-01'-00S.
US Environmental Protection Agenty, Cincinnati, OH.
File: CdNaCICR_100615.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J.-Sumner Reviewed by':
0 a
a Statistical Analyses 2
~ E1vIroflm~nt5Te,¶IngSoIutIons, inc.
Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date: 101612015. Test ID: OdNaCIOR ,Sample ID: REF-Ref Toxicant End Date: 1011312015 Lab ID: ETS-Envir. Testing SoL. Sample-Type: NACL-Sodlum chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Teat Species:. CD-Ceriodaphnia dubla Comments:.*
Cone-mgIlL 1 2 3 4 .5 6 7 8 9 10 D-Control 29.000 28.000 29.000 28.000 29.000 30.000 32.000 32.000 29.000 30.000 600 31 .000 30.000 29.000 32.000 32.000 32.000 30.000 30.000 33.000 28.000 800 32.000 33.000 33.000 27.000 29.000 30.000 31.000 30.000 32.000 29.000 1000 31.000 33.000 29.000 28.000 29.000 26.000 30.000 27.000 28.000 28.000 1200 9.000 11.000 11.000 7.000 7.000 11.000 13.000 8.000 8.000 5.000 1400 2.000 2.000 2.000 3.000 2.000 1.000 3.000 2.000 1.000 2.000 Transform: Untransformed 1-Tailed Isotonic Conc-mgI/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 29.600 1.0000 29.600 28.000 32.000 4.831 10 30.300 1.0000 600 30.700 1.0372 30.700 28.000 33.000 5.104 10 -1.387 2.287 1.813 30.300 I )0000 800 30.600. 1.0338 30.600 27.000 33.000 6.389 10 -1.261 2.287 1.813 30.300 1.0000 1000 28.900 0.9764 28.900 26.000 33.000 7.006 10 0.883 2.287 1.813 28.900 0.9538 "1200 9.000 0.3041 9.000 5.000 13.000 27.217 10 25.976 2.287 1.813 9.000 0.2970 "1400 2000 0.0676 2.000 1.000 3.000 33.333 10 34.803 2.287 1.813 2.000 0.0660 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p
- 0.01) 0.82283 1.035 0.1504 0.06135 Bartlett's Teat indicates eq~ual variances (p = 0.02) 13.1798 15.0863 Hypothesis Test (1-tail. 0,5)1 N4OEC LOEC ChV TUJ MSflu MSfl MSB MCE F-Probh df Dunriett'sTest 1000 1200 1095.45 1.81338 0.06126 1647.58 3.14444 2.0E-44 5,54 Treatments vs D-Control Linear interpolation (200 Resamples)
Point malL SD 95% CL Skew IC05 1001.16 37.4909 906,468 1012 -2.0108 IC10 1018.38 6.35554 1003.22 1027.02 -0.1934 1C15 1031.61 5.9215 1018.58 1042 -0.1085 1.0 1Q20 1046.83 5.58724 1035.25 1057.01 -0.0406 0.9 8
1025 1052.06 5.34593 1051.32 1072.01 0.0262 1C40 1107.74 5.28361 1097.31 1117.31 0.1552 0.8 IQ50 1138.19 5.78951 1127.31 1148.64 0.2091 0.7 0.6 S03 S0.3 0.2 0.1, 0.0 ... - . . .,
-0.1 0 500 1000 1800 Dose mg/L Dose-Response Plot 30 1-tail, 0.05 level of significance 25 208
- .15 10 e , , . .
I5 0 o
o 0 r nd.eod.nt C.
b~4.wby e File: CdNaCICR_1006 ] 5.idec Eatered by: .1.Sumner Reviewed by:
Page 96 of 98
0 Page 5 of 6
~;yi~i ~.
~
CdNaClCR #: 17..9 Species: Ceriodavhniadubia Daily Chemistry: ____________________________________
I m
Day (Analyst identified for each day, performed pH, D.O. and conductivity' measurements only.) I i -
'I I 1 I Concentration Parameter
- I--
pH (S.U.)
DO (rag/L)
Conductivity (u~mhes/cm)
CONTROL *Allnslinity (mg CaCQ 3 IL)
- Hardne5s (inn CaCO~/L)
- Temperature B - *~ih. - 6 pH (s.u.) II 7A~ I 600 mg NaCI/L DOQng/L)
Conductivity IZAI~1LII 7, ~q. I (puhos/em) 1q70 ~37O I.
'*Temperature (°C)
U -
A- Ip pH (s.u.) '4~q3 7~6 1.
L 800 mg NaCI/L DO(mglL)
Conductivity
(~ernhosIcm)
II rtLIo I U
7 jgt~ v15v 1-Ih TCflhJJCflItUT~ ('C) l.A L.qt ,.Y pH (s.U.)
B 1h 224 ,7.q*
1000 mg NaCIIL.
i I conductivity DO~mg/L~
I*Temperature ('C) pH (S.U.)
B 2O~iO 1-'v*. ~II -
1.7 IA,. h --
8.0 2.q.,*.-
VIO 1~6
- qp 7.7 DO (mg/L) e.0 'R g 1200 mg NaCI/L Conductivity
(~+/-mhos/cm)
~43o
- Temnperatulre ('C) 15V1 qp~ 6 pH (S.U.) 7.e7 7,,Rq '1.
DO (mag/L) 1400 mg NaCI/L Cond:etivity F*Temnperature
.. ('C)
I ~'o Initial21L IIJ.*
II FinallIl- nitiallIl J. &I MA' Final
~28tO 24.9
-rInitial
" 11 7"'" =
Final
- Tempeiaturetable lnformation performed located on at the Pagetime of test initiation,
- 1. Alkalinity renewal'performed and hardness or termination by*thebyanalyst the analyst identified identified on theinbench the Daily sheetRenewal specific for each analysis and transcribed to this bench sheet by: ....
- _
Page 97 of 98 SOP AT14 - Exhibit AT14.1, revision 11-01-14
0 Page 6 of 6
¶~ EtVCfiffi.atilTOg5OhALfl~I~~
Species: erodaphniadubia CdNaCICR #: 17_9 Day (Analyst identified for each day, performed pH, D.O. and conductivit measurements only.
3 4 5 6 pflO(S.,.) "7,*1 - "*. " o-
- 'Conductivity 30738 CONTROL *Aflkainty ~2 600 NaC mg (mgCaondctviy
[, .
- j .pH(S.U.) 7.# . 5 1" . i7" q,* Lq -____ -q .
Do (mg,) 7.__7__l,.3 l /i 600mng NaCJIL °*(*-Conducteit iS3o -0 1*7 y
8400 mg NaCI/L Caldc*vt (ishscm)
Initial Final Initial ifFinal jInitial I[Final IInitial IFinal
- Temperatures Information tableperformed at the located on Pagetime of test initiation,
- 1. Alkalinity renew'alperformed and hardness or termination by thebyanalyst the analyst identified in the Daily Renewal.
identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: L_ .. .
SOP ATI4 -*Exhibit ATI4.1, revision 11-01-14 f*
Page .98 ofr 98
Attachment 4 Corrected DMR Page 101T
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR) MAJOR (SR01OMNo24-04 Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
.(.INTEROFFICE OPS-EN-SQN)
TN0026450 [101 T ]F- FINAL
___ SODDY_ .- D._AISY.,IN.37384_ PERMIT NUMBER [DISCHARGE NUMBERJ BIOMONITORING FOR OUTFALL 101 Fgaciliy TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MO~LRING PERIOD IEFFLUENT Froml 15 10 01 fTot 15110131 ] - OOAO ATTN:Millicent Garland NOTE: Read instructions before compIeting this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_______ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 1C25 STATRE 7DAY CHR SAMPLE ********** >1 00.0 **** ****0 1/10CMO CE*RIODAPHNIA MEASUREMENT 23 0 110 CMO TRP3B 1 0 PERMIT ********* **42.8 ********* PERCENT SEMI COMPOS EF FLUENT GROSS RUIENTMINIMUM ANNUAL 1C,2;5 STATRE 7DAY CHR SAMPLE ********* *>100.0 ******** 3 0 1/10CMO PIMEPHALES MEASUREMENT23 0 /10 CM S TRP6C 1 0 PERMIT **** **** ** 42.8 ************* PERCENT SEMI COMPOS EF FLUENT GROSS REQUIREMENT MIMINUM ANNUAL____
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL ExECUTIVE OFFICER I Certify under penaltyof law that this document and allattachments were prepared under my AEELEPHONE Te 15DATE 1
______________________________________ - irection or supervision in accordance with a system designed to assure that qualified Christopher Schwarz personnel properly gather and evaluate the information submitted. Based on my inquiry of the lperson or persons who manage the system, or those persons directly responsible for gathering <-S1ViePsiet42 83701 5 12 0 SieVc Peietthe information, the information submitted is, to the best of my knowledge and belief, true, 423_______843-7001____________
Sit reidet Vce accurate, and complete. I almaware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE -
information, including the possibility of fine end imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT ARA NUMBER YEAR MO DA-Y-TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled October 18 - 23, 2015.