ML14223A779
| ML14223A779 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/11/2014 |
| From: | John Carlin Tennessee Valley Authority |
| To: | Morgan C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Resources |
| References | |
| TN0026450 | |
| Download: ML14223A779 (126) | |
Text
Tcnnessce Valley Authority, Post Office Box 2(01),.Soddy Daisy. l'ennesec 37384-2000 July 11, 2014 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11 th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JUNE 2014 Enclosed is the June 2014 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. Also included is the revised outfall 101 T data with results from May 2014 toxicity for which no adverse effects were observed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
iSin
,¢*ere*'
\\!_ice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
S58 140711 800 - NPDES CORRESPONDENCE July 11, 2014 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR JUNE 2014 Enclosed is the June 2014 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. Also included is the revised outfall 101 T data with results from May 2014 toxicity for which no adverse effects were observed. If you have any questions or need additional information, please contact Millicent Garland by email at mrmoore@tva.gov or by phone at (423) 843-6714.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Since eji2V, Jo~hn.
C hA Site Vice resident Sequoyah Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, OPS 5N-SQN B. E. Brickhouse, BR 4A-C J. T Carlin, OPS 4A-SQN L.S. Cohen, BR 4A-C D. A. Day, POB 2A-SQN T.R. Markum, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 M. McBrearty, OPS 4A-SQN D. B. Nida, BR 4A-C G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN
PERMITTEE NAMFJADDRESS (Include Facility Narme/.ocation i Different)
Name TV EQUOYAH NUCLEAR PLANT Address P.O.
,FX 2000
_.JN'TEROF.FCE OPS-5N-SON-SODDY - D.AISY. TN 37384 Fac-jity TVA -SEUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier NATIONAL POLLUTANT C"' -HARGE ELIMINATION SYSTEM (NPDESI DISCHAR(
INITORING REPORT (DMR)
TN0026450 101 T PERMIT NUMBER I DISCHARGE NUMBER Frm 1 1N ITO1R0.NG_1
_E J QQ.Z T
Fr m4jI os
' Too4 05 3
MAJOR Form App (SUBR 01)
OMB No 2r-,ý-0004 F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE NOTE: Read instructions before comoleting this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.ýFREOUENCYý SAMPLE EXI OF TYPE SAVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSS IC25 STATRE 7DAY CHR SAMPLE
.>100.0 MAXIMUM.......
CERIODAPHNIA MEASUREMENT 23 TRP3B 1
0 PERMIT 43.2 PERCENT
{
SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM I
IANNUAL I IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
1/180 COMPOS' PIMEPHALES MEASUREMENT 2
TRP6C.
0 43.2 PERCENT SEMI COMPOS IEFFLUENT GROSS SAREQUIREMENT MIMINUM ANNUALI SAMPLE
___MEASUREMENT PERMIT REQUIREMENT SAMPLEt MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT
-_t REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT MEASUREMENT REQUIREMENTPRI NAETTEPICPLEEUIEOFCRICriyudrpnlyo a htti ouln n lltttrnrs mer preard ndr
[ NAM/TITL PRICIPAL EXECUTIVE OFFICER I Certify under penalty of law trial this document and all attachmentswe're prepared undern direction or supervision in accordance with a system designed to assure that qualified John T. Carlin Ipersonnel properly gather and evaluate the information submitted Based on my inquiry oft person or persons who manage the system, or those persons directly responsible for gathe the information, the information submitted is. to the best of my knowledge and belief, truo, Site Vice President lacceato. and complete. I am rawae that there are significant penalties tor submitting false O P Tinlonnation, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity sampling occurred May 4-9. The test results were received and are included in this corrected May 10IT my V \\Il//
TELEPHONE I
DATE the
'/i I/IA ring 423 843-7001 14 06 20 SIGNAT ORiF,6 PRINCIPAL EXECUTIVE I
OFFICER OR AUTHORIZED AGENT AREA MBER YEAR MO DAY DMR.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of I
PERMITTEE NArA'rADDRESS (include Facility Name/Location if Different)
Name "ITV
'QUOYAH NUCLEAR PLANT Address P.O ojX 2000
-.JjNTEROFFICE OP S-5-SQSONJ.
SODDY - DAISYTN. 37384 Facility iVA.- SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier PARAMETER AVEI NATIONAL POLLUJTANT DI" 4IARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARC NITORING REPORT (DMR)
(SUBR 01)
TN0026450 101 G 1 F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE 0N I "'O
.E D
EFFLUENT NO DISCHARGE From, 14 06 01 To 14 30y NOTE: Read instruction Form Appr OMB No. 2,
. 0004 s before completing this form.
QUANTITY OR LOADING QUALITY OR CONCENTRATION RAGE MAXIMUM I
UNITS I
MINIMUM TEMPERATURE, WATER DEG.
ýCENTIGRADE 00010 1
0 EFFLUENT GROSS SAMPLE MEASUREMENT AVERAGE MAXIMUM UNITS 39.9 04 NO.
FREQUENCY SAMPLE IEX OF TYPE F
ANALYSIS SNUOUS IAC-f PERMIT REQUIREMENT I
I I
Req. Mon.
DAILY MA.)
TEMPERATURE, WATER DEG.
SAMPLE I
C..............
j C**.
28.9 CENTIGRADE MEASUREMENT 28.9 00010 Z
0 PERMIT
- C*.
- C**
C C*C**
30.5 IINSTREAM MONITORING REQUIREMENT j DAILY I ITEMP. DIFF. BETWEEN SAMP. &
SAMPLE
- 12 UPSTRM DEG.C MEASUREMENT 2
00016 1
S PERMIT 1ALLI 3
EFFLUENT GROSS REQUIREMENT j
_______IDAILY I
30/30 1MODELD1 CONTI CALCTD NUOUS 30/30 CALCTD n
CONTI NUOUS CALCTD FLOW, IN CONDUIT OR THRU SAMPLE i
TREATMENT PLANT MEASUREMENT1 50050 1
0 PERMIT EFFLUENT GROSS REQUIREMENT ICHLORINE, TOTAL RESIDUAL SAMPLE iMEASUREMENT 1749 03 Req. Mon.
MGD DAILY MAX CCC*e**C CCCC*C*C 1
0 30/30 CONTI NUOUS 21/30 FIVE PER WEEK RCORDR RCORDR GRAB CALCTD I
0.017 0.026 19 MG[L o50060 1
0 EFFLUENT GROSS PERMIT REQUIREMENT
- CC****
CCCCCCCC I
0.1 L
Y0.1 MO AV6 DAILY MAX TEMPERATURE - C, RATE OF SAMPLE 0
62*
0 30/*30 CALCTD 182234 1
0 PERMIT F
2 DEG I
CONTI CAL-CTD CHNEMEASUREMENT I ______
iEFFLUENT GROSS REQUIREMENTj
_DALLY MX C_ HR, NUOUS I SAMPLE-ii MEASUREMENT f PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my "l-TELEPHONE DATE
- direction or supervision in accordance with a system designed Io assure hal qualified A
t'..
.e John T. Carlin personnel properly gather and evaluate the information submilter. Based on my inquiry of noI person or persons who manage the system, or those persons directly responsible tor gathenrg S
4:
8 the information, the information submitted is. to the best of my knowledge and belief, true.
I423 843-7Q1 14 07 09 Site Vice President accurate, and complete. I am aware that there are significant penalries for submitting false SIG NTRE OF PRINCIPAL EXECUTIVE 1
Dinformation, including the possibility of fine and imprisonment for kowing vioations OFFIE RR AUTHORIZED AGENT AREA NUMBER YEAR; MO DAY TYPED OR PRINTED J ODE___
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injections occurred: 1. Spectrus CT 1300 (max. conc. 0.04 mg/L, limit 0.05 mg/L). 2. Flogard MS6236 (max. conc. 0.06 mg/L. limit 0.20 mglL), 3. Nalco 73551 (max conc. 0.06 mg/L, limit 2.0 mg/L),
EPA Form 3320-1 (REV 3199)
Previous editions may be used.
Page 1 of 1
PERMITTEENAME/ADDRESS (Include Facilitv Name/Localron if Different)
NATIONAL POLLUTANT DI'HARGE ELIMINATION SYSTEM INPDES)
MAJOR Form App, Name TV EQUOYAH NUCLEAR PLANT DISCHARC
'NITORING REPORT (OMR)
(SUBR 01) 0MB No. k.
-0004 Address P.O.
0..JX 2000 F-FINAL
~~~
.-.I._ROFCOPNS*
_tTENM9E EQP$ESC N)
TN40626466 101 T-IA
--SODDY" DAISY. TN 37384.
PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY Mej.YNO DISCHARGE 7.]EFFLUENT
_~~
1 " 06*__
o**
... NO DISCHARGE * "
ATTN: Spencer Whittier Fromt 14 06 i 01 To 14 06 30 NOTE. Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
F*FQUENI-CYiSAMPLE-I EX OF TYPE
, i EX
- ANALYSIS t
T P MEASUREMENT PERMIT REQUIREMENT I[
SAMPLE MEASUREMENT RPERMIT L
~~REQUIREMENT II NAE/ITE PRINCIPAL EXECUTIVE OFFICER Il Certify under penalty 6f law =ht this document and all attachiments were prepared under my
.K
/ I
/TELEPHONE DATE John T. C
-rlin ldirection or supervision in accordance mth a system designed to assure that qualified
/
I-J John T. Carlinpesonnel properly gather and evaluate the information submitted Based on my inquiry ot line person ons pr o manage the system. or those persons directly responsible for gatho nng esident the information, the information submitted is. to the best of my knowledge and belief, true, SI "E 423 843-7001 14 07 09 Site Vice Pr laccurate, and complete I am aware that there are significant penalties for submitting false SIGNATURg OF PRINCIPAL EXECUTIVE information, i*cuding the possioblity o; fine and imprisonmentr far knowingrvolations I
&FFIGE RORAUTHORIZEDXAGENT AREA NUMBER YEARj MO DAY]
TYPED OR PRINTED CODE 1
COMMENTS AND] EXPLANATION OF ANY VIOLATIONS (Reference all atachments here)
Toxicity was not sampled in June 2014.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1 v
PERMITTEE NAN e'ADDRESS (Include Facility Name/Location if Name TV EQUOYAH NUCLEAR PLANT Address P.O. boX 2000
__..N._TEROFF ICE OPS-5N-SONI SODDY DAISY, TN 73384 Faacili_ TVA - SEOUOYAH NUCLEAR PL ANT Location HAMILTON COUNTY ATTN: Spencer Whittier PARAMETER PH 00400 1
0
- EFFLUENT GROSS Different; NATIONAL POLLUTANT 0*"-ARGE ELIMINATION SYSTEM (AIPDES)
MAJOR Form Appf DISCHARC
- NITORING REPORT (DMR)
(SUBR 01)
OMB No. k.
-0004 TN0026450 103 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND M O= N ITO*Q.RjNGP.E RIO D EFFLUENT 119~AY
ýXMý4 MQJ tA NO DISCHARGE 1
From 14, 06 01 I To 14 06 30 N
NOTE: Read instructions before comoletinq this form.
QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. 'FREQUENCY' SAMPLE SEX*
OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS 7
.9 12 0
13130 GRAB I
9 U
ITHREE/i GRAB MINIMUM MAXIMUM I
EEi SAMPLE MEASUREMENT PERMIT REQUIREMENT I
1!SOLIDS, TOTAL SUSPENDED MESRMNSAMPLE
<6 7
19 0
1, 2130 GRAB3 00530 1
0 PERMIT.
- 30G" C
TOTA SUSENDEREMENT 1_____
30 100 MG/L o~TWIC E/
EFFLUENT GROSS MO AVG DAILY MX MONTH GRAB OIL AND GREASE
}
SAMPLE
<5230 GRAB MEASUREMENT
<19 2
00556 1
0 PERMIT 15 20 MG!L
- TWICE, GRAB IEFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH
- FLOW, IN CONDUIT OR THRU SAMPLE 1.072 1.341 03 0
30/30 RCORDR TREATMENT PLANT MEASUREMENT 03 30/30_______
50050 1
0
[
PERMIT 1 Req. Mon. I Req. Mon I
MGD t
1 SEE R
EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX PERMIT SAPLE-MEASUREMENT PERMIT REQUIREMENT { _
SAMPLE I
MEASUREMENT PERMIT I
MEASUREMENT PERMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 11 Certily under penally of law thal this document and all a direction or supervision in accordance with a system dos John T. Carlin lpersonnel properly gather and evaluate the information s person or persons who manage the system. or those per the mlnormation. the information submitted is, to the best Site Vice President accurate, and complete. I am aware that there are signifi L.
inflomation. inctuding the possibility of line and nmprison TYPED OR PRINTEDl ttachiments were prepared under my 1/
')tii/jj TELEPHONE DATE igned to assure that qualified
/I i
ubmiltod. Based on my inquiry of tne
\\
sons directly responsible fcr gatllering 40 of my knowledge and belief, LI1e.
42 1
cant penallies for submitting false SIG"NTURE OF PRINCIPAL EXECUTIVE
__I_"
men-, for Kno'rcnt iiolaion0.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEARI MO I CODE LEAI COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAMFIADDRESS (Include FacilityfName/Location it Different)
Name TV EQUOYAH NUCLEAR PLANT Address P.O. _,.,X 2000
--- INjTEROFFICE OPS-5N-SQN.
SODDY-DAISY TN._37384 Fs,-ilih,
'T*/f
- *I;;I hIWAI IMI InI *AO Di AMTr NATIONAL POLLUTANT 0'-HARGE ELIMINAIION SYSTEM (NPOES)
MAJOR DISCHARC INITORING REPORT (DMR)
(SUBR F -
)
TN0026450 110 G F-FINAL I
PERMIT NUMBER I DISCHARGE NUMBER RECYCLED COOLING WATER Form App OMB NO. 2,
,-0004 Location HA MILTONCO.UN TY ATTN: Spencer Whittier 7
PARAMETER
_ MONITORJ N
GLiEG Rý,
EFFLUENT From YEARMO.
NO DISCHARGE FX-I Frm 06To0I~ T o L 14 j0 6 130V NOTE: Read instructions before comoleting this form.
QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCYý SAMPLE I__
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM NITS ANALYSIS TEMPERATURE, WATER DEG.
- CENTIGRADE 00010 1
0 EFFLUENT GROSS VALUE I
SAMPLE MEASUREMENT.
PERMITUIREN REQUIREMENT bb
- A ****
A
- 04
- AC* * * *
- REPORT DAILY MX 04 1 DEGOC CONTIN CALCTD UOUS TMEAUEWAEDE.
SAMPLE 04~~******
- I JCENTIGRADE WATER DEG.
MEASUREMENT S
04 I
00010 Z
0 PEMT
.e**,..*****
05 DEG C CONTIN CALCTD 0INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. &
SAMPLE P
04 1UPSTRM DEG.C MAUEENT I________________________
.00016 1
0 PERMIT
- ena
- n DEG C CNTIN JCALCTDý
ýEFFLUENT GROSS VALUE REQUIREMENT DAILY MX uOus FLOW, IN CONDUIT OR THRU SAMPLE 03 ITREATMENT PLANT MEASUREMENT 0
150050 1
0 PERMIT 1
1 Req. Mon.
MGD 1
CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT_
DAILY MX UOUS JCHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 19__
L_
150060 1
0 PERMIT
- a**
aaan Iaan 0.11 0.1 MGIL Five per CALCTD iEFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MXj Week
[TEMPERATURE - C, RATE OF SAMPLE 04.................**"
CHANGE MEASUREMENT 04 82234 1
0 PERMIT 2
DEG C J ******
..aa****
1 CONTIN CALCTD IEFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS SAMPLE MEASUREMENT PERMIT i
REQUIREMENT
_1 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law fhe:thies documenti and all attachmnents were prepared under my TELEPHONE I
DATE d1direction or supervision in accordance with a system designed to assure that qualified John T. Carlin 1personnel properly gathoe and evaluate the information submitted Based on my mnquiry of the/
'Person or persons who manage the system, or those persons directly responsible for gatheriN SI jt c P ýsid ent 1
1 7
0 e c ie information, the information submitted is. to the best of my Knowledge andl belief, true.
i 423 843-70 Site Vice President accurate, and complete I am aware that there are significant penalties *ot subnmliing false iSI NATURE Of PRINCIPAL EXECUTIVE
'4_43 01 4._09 TPED_____RINTED dinformation. including the possibility of fine and impriscnment for knowing violations.
6'FFIdE OR AUTHORIZED AGENT
-AREA
.MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a/ attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different)
Name TV "EQUOYAH NUCLEAR PLANT A d d re s s P.C,.
-JX 2 0 0 0
.,(.INTEROFFICE OPS-5N-SQN)
SODDY - DAISY. TN 37384 Facili.ty A -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier I
PARAMETER I
AVEI NATIONAl POLLUTANT Oo"HARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form App DISCHARI
)NITORING REPORT (IOMR)
(SUR 01)
OMB No.
-0 F-TN0026450 110 1 F-FINAL PERMIT NUMBER DISCHARGE NLUMBER RECYCLED COOLING WATER SMlONITO..
GP E RLOD EFFLUENT NO DISCHARGE XX Froml4 06 ' 01 To 14 06 30 NOTE: Read instructions before completina this form.
004 QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. ýFREQUENCY SAMPLE I EX OF I
TYPE S
,ANALYSIS i
IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1
0 0
EFFLUENT GROSS VALUE lIC25 STATRE 7DAY CHR PIMEPHALES jTRP6C 1
0 0
- EFFLUENT GROSS VALUE SAMPLE I
MEASUREMENT PERMIT REQUIREMENT RAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM I
SAMPLE
.MEASUREMENT PERMIT REQUIREMENT r-
=
SAMPLE T
. MEASUREMENT PERM[T REQUIREMENT I"
SAMPLE MEASUREMENT RPERMIT REQUIREMENT I
SAMPLE MEASUREMENT PERMrI REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT j
,__R PERMIT
_/
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 11 Cerrldy under penalty el law that this document and all attachments were prepared under my directror or supervision in accordance with a system designed to assure trial qualified John T. Carlin personnel properly gather and evaluate the information submitted Based on my inquiry o1 the person or persons who manage the system, or those persons directly responsib e for gathering
,tVee information, the information submitted is, to the best of my knowledge and belIef, truo, Site ice residntIaccuratre; and complete I am aware that there are significant penalties for submitting false
'information. including Ite possibility of fine and impnsonment for Pnowing violat;ons TYPED OR PRINTED TELEPHONE DATE
-'iV ident 423 843-7001 I 14 07 09 SIGNA'TUE"OF PRINCIPAL EXECUTIVE I
OFFICER OR AUTHORIZED AGENT I AREA NUMBER Y
MO DAY
__________________I CODE ER COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAMEIADDRESS (Include Facility Name/Location if Different)
NATIONAL POLLUTANT DC'qHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form App' Name TV EQUOYAH NUCLEAR PLANT DISCHARI
)NITORING REPORT (DMR)
(SUBR 01)
OMB No. ý
.0004 Address P.O_ -- X 2000 IN.TEROFFI.CE OPS-5N-SqN)
TN00264506 118 G F-FINAL SODDY-DAISY, TN 37384 PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Fa.Jlitv TVA - SEQUOYAN NUCLEAR PLANT._._._._._.
Location HAMILTON COUNTY O
OR N ERIOD EFFLUENT RY
,I0_
.DY
. NO DISCHARGE F 1 ATTN: Spencer Whittier From 14
- 06. ! 01, To 14 06 130 Nf NOTE: Read inlslructionls before completing Ihis form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION INO.
FREQ-UE`NCYT!SA`MPLE
_________________________EX OF ITYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT 19 i00300 1
0 I
PERMIT 2
MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT F
MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 1
00530 1
0 PERMIT 100 MGIL TWICE/I GRAB
!EFFLUENT GROSS REQUIREM NT
_DAILY MX WEEK ISOLIDS, SETTLEABLE SAMPLE MEASUREMENT F25
,00545 1
0 PERMIT j
MLIJL ONCE/
GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH iFLOW, IN CONDUIT OR THRU SAMPLE
- e**
TREATMENT PLANT MEASUREMENT 03 50050 1
0 PERMIT Req. Mon.
Req. Mon.
MGD O
E*****4
,****4, ONCE/
ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX BATCH I p
i NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law* that this document end all attachments wver prepared unrer my "oirection or supervision in accordance with a system dosigned to assure that qualified F
John T. Carlin personnel properly gaiher and evaluate lie nrformation submillid. Based on my inquiry of they person or persons who manage the system, or those persons alretly responsibte for gatnering the information, lhe information submitted is. to [re best of my kno,,.edge and belief true.
Site Vice President accuate. atnd complete I am aware tiat there are significant penalties lor suomrttign talse
¶ StN TU OF PF TYPEDOR PRINTED information, including t0o e ossibitty of fine and imprisonment for knoring violations OFFICER OR AL COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference agtl attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320.1 (REV 3199)
Previoua editions may be used TELEPHONE DATE lP'e~ilJ~ef 423 843-7001 14 07 09 tINCIPAL EXECUTIVE JTHORIZED AGENT AREA NUMBER
- YEAR MO 0DAY
_________coo_
I IOQ I
Page 1 of 1
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: June 09, 2014
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 7. 1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: May 04- 09, 2014
- 10. Average Flow on Days Sampled (MGD): 1716.83, 1713.50, 1717.08
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: May 06 - 13, 2014
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC 25 = 43.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: IC25 > 100%
Ceriodaphnia dubia: IC 25> 100%
UV treated Outfall 101: Pimephales promelas: ICL > 100%
Page 1 of 117
- 18. Facility
Contact:
Spencer Whittier Phone#: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Exposures to samples collected May 04 - 09, 2014 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 2 of 117
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3.
Sample Information:
Date Date Date (MM-DD-YY)
(MM-DD-YY)
Arrival Initial (MM-DD-YY)
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 101 05-04-14 0650 to 05-05-14 1340 3.5, 2.8'
<0.10 05-06-14 1237 05-05-14 0550 05-07-14 1142 05-04-14 0703 to 05-06-14 1237 Intake 0-51063 05-05-14 1340 2.7
<0.10 057-412 05-05-14 0603 05-07-14 1142 101 05-06-14 0600 to 05-07-14 1220 0.7, 041
<0.10 05-08-14 1215 05-07-14 0500 05-09-14 1138 Intake 05-06-14 0615 to 05-07-14 1220 0.3
<0.10 05-08-14 1215 05-07-14 0515 05-09-14 1138 05-10-14 1227 101 05-08-14 0610to 05-09-14 1240 2.0,2.0t
<0.10 05-11-14 1138 05-09-14 0510 05-12-14 1137 05-10-14 1227 05-08-14 0624 to051-427 Intake 05-08-14 0524 05-09-14 1240 1.8
<0.10 05-11-14 1138 05-09-14 0524 05-12-14 1137
- TRC = Total Residual Chlorine tSarnples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.00C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page 3 of 117
Test Organisms:
Pimephales promelas Aguatox, Inc.
19.42 - 20.62 hours7.175926e-4 days <br />0.0172 hours <br />1.025132e-4 weeks <br />2.3591e-5 months <br /> old Ceriodaphnia dubia In-house Cultures
< 24-hours old
- 1. Source:
- 2. Age:
Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8. Test Temperature: Outfall 101:
10 1
05-06-14 1237 ET 05-06-14 1225 ET 05-13-14 1246 ET 05-13-14 1230 ET Mean = 24.70C (24.2 - 25.00C) 05-06-14 0942 ET 05-13-14 1008 ET Mean = 24.90C (24.7 - 25.3 0C)
Test Temperature: UV-Treated Outfall 101:
Mean = 24.8°C (24.2 - 25.30C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinnevville, CA).
Page 4 of 117
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 06 - 13, 2014 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 90 85 85 Test Solutions Mean Dry Weight (mg)
Tet Efluetis (replicate number)
(% Effluent) 1 2
3 4
Mean Control 0.772 0.794 0.759 0.689 0.754 10.8%
0.987 0.869 0.841 0.906 0.901 21.6%
0.938 0.777 0.910 0.968 0.898 43.2%
0.910 0.828 1.133 0.888 0.940 86.4%
0.935 0.892 0.965 0.948 0.935 100.0%
0.923 0.986 0.896 0.842 0.912 Intake 0.364 0.728 0.728 0.805 0.656 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100I/LC 50: TUc = 100/IC 25 Page 5 of 117
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 06 - 13, 2014 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions Solutns 1
2 3
4 5
6 7
(% Effluent)
Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Test Soluetis Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 31 29 30 29 28 30 29 30 32 31 29.9 10.8%
32 35 32 32 32 29 30 31 33 30 31.6 21.6%
33 30 33 33 31 32 31 29 33 33 31.8 43.2%
36 32 35 32 34 33 33 33 35 36 33.9 86.4%
33 32 38 32 36 34 34 37 37 35 34.8 100.0%
37 38 36 35 33 36 34 36 35 36 35.6 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50: TUc = 100/IC25 Page 6 of 117
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 06 - 13, 2014 using water from Intake Percent Surviving Test (time interval used - days)
Sltos 1
2 3
4 5
6 7
(% Effluent)
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Tet Soluetis Data (re plicate number)
(Ef2 3
4 5
6 7
8 9
10 Mean Control 28 29 30 30 29 33 32 30 30 30 30.1 Intake 33 31 30 31 33 30 32 30 33 31 31.4 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC25 Page 7 of 117
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 06 - 13, 2014 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
l1o 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 98 98 Test Solutions Mean Dry Weight (mg)
(% Effluent)
(replicate number)
(% Effluent) 1 2
3 4
Mean Control 0.756 0.650 0.661 0.737 0.701 10.8%
0.836 0.749 0.747 0.876 0.802 21.6%
0.796 0.724 0.741 0.746 0.752 43.2%
0.656 0.778 0.751 0.867 0.763 86.4%
0.662 0.808 0.753 0.795 0.755 100.0%
0.785 0.795 0.788 0.866 0.809 Intake 0.819 0.740 0.815 0.721 0.774 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25)
Pimephalespromelas May 06 - 13, 2014 1247 7-days KCI 0.82 g/L Ceriodaphnia dubia May 06 - 13, 2014 0930 7-days NaCI 1.05 g/L Page 8 of 117
(Q (a
PHYSICAIJCHEMICAL
SUMMARY
Water Chenistry Mean Values and Ranges or Pimephalespromelas and Ceriodaphnia dubia Tests, Non-lreated Sequoyah Nuclear Plant (SQN) OutfU 101 performed May 06-13, 2014.
Test Sample ID Temperture (C)
Dissolved O ygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (Amhos/cm) (01g/L CaCO3) (mg/L CaCO3)
Chlorine (mg/L)
Control 24.8 24.5 7.7 7.6 7.98 7.67 305 60 90 24.7-24.9 24.3 - 24.6 7.6 -
7.9 7.3 -
8.0 7.90-8.03 7.56-7.88 300 -
310 58-62 86
-94 10.8%
24.8 24.5 7.9 7.5 7.98 7.65 306 24.7 - 25.0 24.2 - 24.8 7.8 8.1 7.3 8.0 7.88 - 8.04 7.55 - 7.79 295 -
313 24.8 24.5 7.9 7.5 8.01 7.65 288 21.6%
24.7 - 25.0 24.4 - 24.7 7.8 -
8.2 7.2 -
8.0 7.95 - 8.06 7.53 - 7.81 278 -
303 43.2%
24.8 24.6 7.9 7.5 8.00 7.71 260 1
24.7 - 25.0 24.5 - 24.7 7.8 -
8.2 7.2 -
7.9 7.94 - 8.07 7.52 - 7.98 249 -
298 Q
24.9 24.5 7.9 7.5 8.00 7.66 197 24.7 - 25.0 24.4 - 24.7 7.7 -
8.2 7.1 7.9 7.95 - 8.07 7.52 - 7.85 182 -
221 100.0%
24.9 24.6 7.9 7.5 8.01 7.67 174 66 71
< 0.10 24.8 - 25.0 24.4 - 24.7 7.7 -
8.2 7.1 7.9 7.95 - 8.07 7.52 - 7.86 160 -
188 65 67 64 78
<0.10 -
<0.10 Intake 24.9 24.6 8.0 7.4
. 8.03 7.69 189 66 71
<0.10 24.7 - 25.0 24.3 -
24.7 7.7 -
8.2 7.1 7.9 7.95 - 8.10 7.56 - 7.87 178 -
207 65 67 68 74
<0.10 -
<0.10 Control 24.8 25.0 7.7 7.9 7.98 7.91 305 60 90 24.7 - 24.9 24.9 -
25.2 7.6 -
7.9 7.8 -
8.1 7.90 - 8.03 7.86 -
8.00 300 -
310 58 62 86 94 10.8%
24.9 25.0 7.9 8.0 7.98 7.92 306 24.7 - 25.0 24.8 -
25.3 7.8 -
8.1 7.8 -
8.1 7.88 - 8.04 7.86 -
8.00 295 -
313 2.%
24.9 24.9 7.9 8.0 8.01 7.91 288
___21"6_
24.7 -
25.0 24.8 -
25.0 7.8 -
8.2 7.8 -
8.1 7.95 - 8.06 7.85 - 7.97 278 -
303 4.%
24.9 24.9 7.9 8.0 8.00 7.91 260 43.2%
24.8 -
25.0 24.8 -
25.1 7.8 -
8.2 7.8 -
8.1 7.94 - 8.07 7.82 - 7.98 249 -
298 24.9 24.9 7.9 8.0 8.00 7.90 197 24.8 -
25.0 24.8 -
25.1 7.7 -
8.2 7.8 -
8.1 7.95 -
8.07 7.79 - 7.98 182 -
221 100.0%
25.0 25.0 7.9 7.9 8.01 7.90 174 66 71
<0.10 24.9 -
25.1 24.9 -
25.1 7.7 -
8.2 7.7 -
8.1 7.95 -
8.07 7.79 -
7.98 160 -
188 65 67 64 78
<0.10 -
<0.10 Intake 24.9 25.0 8.0 7.9 8.03 7.95 189 66 71
< 0.10 24.8 -
25.0 24.8 -
25.3 7.7 -
8.2 7.8 8.1 7.95 -
8.10 7.87 -
8.09 178 -
207 65 67 68 74
<0.10 -
<0.10 Overal temperature CC Pimephalespromelas Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.2 24.7 Maximum 25.0 25.3
10 lb 0
PHYSICAIJCIHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges fir Pimephalespromelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfil 101 perfbrmed May 06-13, 2014.
Test Sample ID Temperature CC)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness
- Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCOA) (mg/L CaCOA) Chlorine (nag/L)
Control 24.9 24.6 7.9 7.4 8.01 7.69 315 61 88 24.7 - 24.9 24.3 -
24.7 7.9 -
8.0 7.0 -
7.9 7.94 -
8.06 7.61 - 7.89 313 -
321 60 63 80 96 10.8%
24.9 24.6 7.8 7.4 7.99 7.69 305 24.8 - 25.0 24.5 -
24.7 7.6 -
8.0 7.0 -
7.9 7.94 -
8.06 7.63 - 7.84 297 -
320 25.0 24.6 7.8 7.4 8.00 7.66 289 24.8 - 25.1 24.4 -
24.7 7.6 -
8.0 7.0 -
7.9 7.95 -
8.06 7.59 - 7.84 270 -
306 43.2%
25.0 24.6 7.8 7.3 8.00 7.67 261 24.9 - 25.1 24.3 -
24.7 7.6 -
8.1 7.0 -
7.8 7.93 -
8.06 7.57 - 7.84 240 -
297 86.4%
25.0 24.5 7.9 7.3 8.00 7.65 201 86"4%
24.9 -
25.1 24.2 - 24.7 7.6 -
8.1 6.9 -
7.8 7.94 - 8.07 7.56 - 7.85 187 -
216 100.0%
25.1 24.5 7.9 7.3 8.00 7.65 177 63 67
< 0.10 25.0 -
25.3 24.2 - 24.7 7.6 -
8.2 6.8 -
7.8 7.94 - 8.08 7.53 - 7.89 164 -
190 61 65 64 70
<0.10 -
<0.10 Intake 25.0 24.5 7.9 7.3 8.01 7.71 191 66 71
< 0.10 24.9 -
25.2 24.4 - 24.7 7.7 8.2 7.0 -
7.9 7.95 - 8.11 7.61 -
7.89 180 -
206 65 67 68 74
<0.10 -
<0.10
- Note: Total residual chlorine was performed on non-treated Ouffall 101 and Intake samples.
Overall temperature (C)
Average Minimum Maximum Pimephalespromelas 24.8 24.2 25.3
SUMMARY
/ CONCLUSIONS Exposures to samples collected May 04 - 09, 2014 from Outfall 101 resulted in no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 11 of 117
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821 -R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-82 l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-m-L of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 117
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B-2000.
- 5. The pH was measured by SM 4500-H+ B-2000.
- 6. Conductance was measured by SM 2510 B-1997.
- 7. Alkalinity was measured by SM 2320 B-1997.
- 8. Total hardness was measured by SM 2340 C-1997.
- 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-82 l-R-02-013. Any known deviations were noted
.during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCI.
- 2.
Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.
Sodium Chloride (NaCI crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 117
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82 l-R-02-013 (October 2002).
- 3.
Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 117
Sequoyah Nuclear Plant Biomonitoring May 06 - 13, 2014 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 9, 2014 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat
_ _TRC Azole 03/12/1998 0.016 03/13/1998 0.0,15 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 7
09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 06013 0.055 0.019 09/12/1998
< 0.001 0.044 0.015 09/13/1998
< 0.001 0.044 0.015 09/14/1998 00.08:
0.044 0.015 02/22/1999
< 0. 001 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999
< 0.001 02/28/1999
< 0.001 08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070 0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.006 08/24/1999 0.016 0.067 0.023 0.020 Page 16 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 9, 2014 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L
,TRC Phosphate Copolymer DMAD mg/L Quat TRC
"_Azole 01/31/2000
< 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000
< 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000
< 0.0057 0.055 0.019 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000
< 0.0088 0.055 0.019 07/30/2000
< 0.0076 0.055 0.019 07/31/2000
< 0.0152 0.055 0.019 0.006 08/01/2000
< 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.005 12/13/2000
< 0.0120 0.025 0.020 12/14/2000
< 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000
< 0.0036 0.025 0.020 12/17/2000
< 0.0036 0.025 0.020 08/26/2001 0.017 0.06 0.021 0.006 08/27/2001
<0.0096 0.06 0.021 0.005 0.021 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 0.020 0.005 0.021 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001
<0.005 0.059 0.020 11/25/2001
<0.0044 11/26/2001
<0.0119 0.024 0.02 0.005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001
< 0.0043 0.024 0.02 12/09/2001
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001
<0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 1
Page 17 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 9, 2014 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 0.0079 0.023 0.02 0.006 01/03/2002 0.0042 0.023 0.014 01/04/2002 0.0124 0.024 0.014 0.009 01/05/2002
< 0.0042 01/06/2002 0.0042 01/07/2002
< 0.0089 0.024 0.014 0.006 02/24/2002 0.004 02/25/2002 0.004 0.023 0.023 02/26/2002 0.0143 0.023 0.023 0.007 02/27/2002
< 0.0041 0.023 0.023 02/28/2002
< 0.0041 0:024 0.008 03/01/2002
< 0.0041 0.024 0.008 05/05/2002 05/06/2002 G-0.058 0.02 0.014 05/07/2002 0.058 0.02 0.015 05/08/2002 0.056 0.019 05/09/2002 0.057 0.02 0.014 05/10/2002 0.056 0.019 08/04/2002
<0.0058 08/05/2002
<0.0058 0.053 0.018 0.025 08/06/2002 0.0092 0.053 0.018 08/07/2002
<0.0107 0.055 0.019 0.007 08/08/2002
<0.0061 0.055 0.019 08/09/2002 0.0152 0.054 0.018 0.00.8 10/06/2002
<0.00497 10/07/2002 0.0153 0.054 0.018 0.009 10/08/2002
<0.0092 0.054 0.018 0.007 10/09/2002 0.0124 0.053 0.018 0.009 10/10/2002 0.0134 0.054 0.018 0.009 10/11/2002
<0.0042 0.054 0.018 01/12/2003
<0.0035 01/13/2003
<0.006 0.025 0.019 0.009 01/14/2003
<0.0118 0.026 0.020 01/15/2003
<0.0063 0.026 0.020 0.009 01/16/2003
<0.0034 0.026 0.020 01/17/2003 1 <0.0034 0.026 0.009 I
04/06/2003
<0.0073 04/07/2003
<0.0189 0.021 04/08/2003
<0.0117 0.021 04/09/2003
<0.0139 0.021 0.016 04/10/2003
<0.0113 0.021 0.018 04/11/2003 1 <0.0073 0.022
-I Page 18 of 117
.Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 9, 2014 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 0.0045 06/16/2003 0.0037 0.057 0.020 0.022 06/17/2003 0.0048 0.041 0.014 0.024 06/18/2003 0.0048 0.041 0.014 0.024 06/19/2003 0.0085 0.058 0.020 0.025 06/20/2003
< 0.0048 0.058 0.020 0.025 08/03/2003
<0.0050 08/04/2003
<0.0050 0.058 0.020 08/05/2003
<0.0051 0.057 0.020 0.025 08/06/2003
<0.0084 0.057 0.020 0.025 08/07/2003 0.0129 0.057 0.020 0.024 08/08/2003 0.0153 0.057 0.020 0.009 10/05/2003
<0.0043 0.057 0.020 10/06/2003
<0.0043 0.057 0.020 0.025 10/07/2003
<0.0090 0.057 0.020 0.025 10/08/2003
<0.0106 0.057 0.020 0.025 10/09/2003 0.0181 0.026 0.022 0.025 10/10/2003 0.0183 0.026 0.024 0.009 02/01/2004 0.0093 0.027 0.009 02/02/2004
<0.0034 0.026 0.009 02/03/2004
<0.0034 0.026 0.009 02/04/2004 0.0124 0.026 0.009 0.009 02/05/2004
<0.0034 0.026 0.009 02/06/2004 0.0105 0.026 0.009 0.010 05/04/2004
<0.0123 0.026 0.019 0.025 05/05/2004
<0.0144 0.026 0.014 0.009 0.025 05/06/2004
<0.0146 0.037 0.013 0.025 05/07/2004 0.0227 0.058 0.020 0.009 0.025 05/08/2004 0.016 0.060 0.021 05/09/2004
<0.0104 0.058 0.020 07/04/2004 0.0217 0.057 0.019 07/05/2004
<0.0085 0.057 0.020 0.009 07/06/2004
<0.0077 0.058 0.020 0.031 07/07/2004 0.0252 0.056 0.019 0.031 07/08/2004 0.0223 0.057 0.019 0.009 07/09/2004 0.0182 0.057 0.020 0.009 1
Page 19 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998-May 9, 2014 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypoehlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e
TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004
<0.0187 0.000 0.014 11/08/2004
<0.0192 0.047 0.030 11/09/2004
<0.0233 0.048 0.016 0.041 11/10/2004
<0.0149 0.047 0.016 0.041 11/11/2004
<0.0149 0.049 0.017 0.043 11/12/20041 1
<0.0253 0.048 $
0.017 0.042 02/06/2005
<0.0042 0.028 0.010 02/07/2005
<0.0116 0U028 0.010 0.007 02/08/2005
<0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005
<0.0042 0.028 0.010 02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 06/06/2005 0.0043 0.037 06/07/2005 0.0103 0.037 06/08/2005 0.0295 0.037 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 0.036 07/19/2005 0.0163 0.026 0.009 0.036 07/20/2005 0.0209 0.026 0.009 0.014 0.036 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 0.018 0.014 10/30/2005 0.0068 10/31/2005 0.0112 11/01/2005 0.0104 0.035 11/02/2005 0.0104 0.036 11/03/2005 0.0117 0.036 11/04/2005 0.0165 0.035 11/14/2005 0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 0.0231 11/18/2005 0.0200 11/19/2005 0.0116 I
Page 20 of 117
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 9, 2014 Date Sodium Towerbror PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC EO/PO Phosphate 11/12/2006 0.0055 11/13/2006 0.0068 0.037 11/14/2006 0.0143 0.037 11/15/2006 0.0068 0.037 11/16/2006 0.0267 0.037 11/17/2006 0.0222 11/26/2006 0.0188 11/27/2006 0.0138 11/28/2006 0.0120 11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 0.015 05/29/07 0.036 0.015 05/30/07 0.0084 0.017 0.036 0.015 05/31/07 0.0103 0.036 0.015 06/01/07 0.0164 0.017 0.036 0.015 06/02/07 0.0305 0.015 12/02/07 0.0241 12/03/07 0.0128 12/04/07 0.0238 12/05/07 0.0158 12/06/07 0.0162 12/07/07 0.0175 04/13/08 0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.0149 10/26/08 0.0260 10/27/08 0.0151 0.017 10/28/08 0.0172 0.041 10/29/08 0.0154 0.018 0.041 0.030 10/30/08 0.041 0.030 10/31/08 0.0086 0.041 0.030 Page 21 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 -May 9, 2014 Date Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Speetrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L
-PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 0.0197 0.017 02/09/09 0.0237 0.017 02/10/09 0.0104 0.021 02/11/09 0.0155 0.017 02/12/09 0.0106 0.017 02/13/09 05/10/09 0.0129 05/11/09 0.0415 0.0446 05/12/09 0.0053 0.0396 05/13/09 0.0049 0.0396 05/14/09
<0.0141 0.0397 05/15/09
<0.0160 11/15/09 0.025 11/16/09 0.0152 11/17/09 0.0255 11/18/09 0.0306 11/19/09 0.0204 11/20/09 0.0093 05/09/10 0.0192 05/10/10 0.0055 05/11/10 0.0100 0.039 05/12/10 0.0171 0.039 05/13/10 0.0041 0.039 05/14/10 0.0099 0.039 Page 22 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998-May 9, 2014 Date Sodium Towerbror PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo-mg/L 222 mg/L mg/L
-PFmg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly-DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos-mer EO/PO Quat Phos-Phosphate TRC phate phate 10/31/10 11/01/10 0.0122 11/02/10 0.0112 11/03/10 0.0163 11/04/10 0.0107 11/05/10 0.0132 05/01/2011 05/02/2011 0.04 05/03/2011 0.04 05/04/2011 0.0155 0.04 05/05/2011 0.0179 0.04 05/06/2011 0.0089 11/06/2011 0.0168 11/07/2011 0.0225 11/08/2011 0.0141 11/09/2011 0.0239 11/10/2011 0.0242 11/11/2011 0:0231 05/06/2012 05/07/2012 05/08/2012 0.041 05/09/2012 0.0145 0.041 05/10/2012 0.0298 0.041 05/11/2012 0.0174 08/12/2012 0.029 08/13/2012 0.0256 0.028 0.037 0.029 08/14/2012 0.0209 0.037 0.029 08/15/2012 0.0279 0.028 0.029 08/16/2012 0.0076 0.029 08/17/2012 0.0446 0.032 05/12/2013 0.0099 05/13/2013 0.064 05/14/2013 0.0091 0.039 0.064 05/15/2013 0.0096 0.039 0.064 05/16/2013 0.0229 0.032 05/17/2013 1 0.0063 1
I I
0.032 Page 23 of 117
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - May 9, 2014 Date Sodium Towerbror PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-i50M MSW Floguard Hypo-mg/L 222 mg/L mg/L
-PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly-DMAD Azole Quat mg/L mg/L Quat mg/L mg/L mg/L Phos-mer EO/PO Quat Phos-Phosphate TRC phate phate 09/15/2013 0.03 09/16/2013 0.0072 0.0379 0.03 09/17/2013 0.0107 0.036 0.0379
.0.03 09/18/2013 0.0217 0.036 0.0379 0.03 09/19/2013 0.0172 0.03 09/20/2013 0.0173 0.03 05/04/2014 0.0118 05/05/2014 0.0112 05/06/2014 0.0096 05/07/2014 0.0164 05/08/2014 0.0235 05/09/2014 0.0110 Page 24 of 117
Sequoyah Nuclear Plant Biomonitoring May 06 - 13, 2014 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 25 of 117
BIOMONITORING CHAIN OF CUSTODY RECORDP Page of Client: TVA Project Name: Sequoyah NP Toxicity Environmental Testing Solution, Inc.
351 Depot Street.
AsheviUe, NC 28801 Delivered By (Circle One):
FedEx UPS Bus Client P.O. Number: N/A Facility Sampled: Sequoyah NP Other (seiy:'e^
s-h Lft Phone:
828-350-9364 General Comments:
75-a i dt-4pits' frp,, rf ieoi 01
-ll Ac ~ ~
~
~
A~~
/doCy rýýV 1
A54zrrýA&&we or Aewlp
-4j',4e I
I I
I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6*C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 26 of 117
~TS Page J00 Whole Effluent Sample Receipt Log
'Sample temperature performed usingSample ReceMng Themometer. SN 130580151 Dot" Time Received Received
- Sample Project Sample Sample name and description state Comments Received Received by from Temp. ('C number number at 05-05-14 1117 J. Sumner L Keenan 1.4 9772 140505.01 Waynesville WWTP - Pp NC 05-05-14 1340 J. Sumner TVA Courier 3.5/2.8 9773 140S05.02 TVA - Se.uoyah Nuclear Plant - 101 TN 05-05-14 1340 J. Sumner TVA Courier 2.7 9773 140505.03 WA-Suoh Nudear Plant - Intake TN Page 27 of 117 SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1
of 1
Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number N/A Asheville, NC Other (specify):.,C N
. vetw /
Facility Sampled: Sequoyah NP 28801 General Comments:
NPDES Number: TN0026450 Phone:
828-350-9364 fo 077/!iy Collected By: "7t Fax:
828-350-9368 S*-'*.'"
W tbis.4. &'7vMI SA*j;JV "at o-Adi~
0-J.,4 d
Field Identification /
Number &
Flow Rain Event?
Sample Description Grab/Comp.
Collection Daie/Time Volume (MGD)
(Mark as Appropriate)
Laboratory Use Collected(Mr sApoite if No Trace ETS Log Arrival Temp.
By Time Appear-Etadt..nd.Yes No Trace Number (L
E:
ance
£1"/V,,,
s'/7,/,"
SQN-,Ol-TOX-.2 Comp S.4, 2(2.5gal)
I-40-1 x
SQN-INT-TOX-.
Comp 1(2.5 gal)
X
.,P "c
Sample Custody - Fill In From Top Down 4s r.a ef-1VzYrtQV*
SDA."
tCCC'J Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Tir
_~_*lb A--1
/
,,-o?-,/
"C)-
6..vŽ-
I i
I II IIII ii i
- A Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 28 of 117
mgiLls Page...j3 Whole Effluent Sample Receipt Log
- Sample temperature performed using Sample ReceMng Thermometer SN 130580151 Date Time Received Received
'Sample Project Sample Sample name and description state comments Received Received by from Temp.
m) number numberp nt 05-07-14 0952 K. Keenan Fed - Ex 0.5 9792 140507.01 CORR - ERI - AAF McQuay International NC 05-07-14 0952 K. Keenan Fed - Ex 0.9 9793 140507.02 Duke Energy - Belews Creek 55 NC 05-07-14 0952 K. Keenan Fed - Ex 2.8 9794 140507.03 Enviro Chemists - Bladenboro WWTP NC 05-07-14 0952 K. Keenan Fed - Ex L8 9795 140507.04 Brunswick County NC 05-07-14 0952 K. Keenan Fed - Ex 1-2 9796 140507.05 Statesville - Sparks Road WTP NC 05-07-14 0952 K. Keenan Fed - Ex 1.0 9797 140507.06 United Water - Enfield WWTP NC 05-07-14 0952 K. Keenan Fed - Ex 0.3 9798 140507.07 Duke Energy - Marshall SS NC 05-07-14 0952 K. Keenan Fed - Ex 0.7 9799 140507.08 Duke Energy - McGulre NS 005 NC 05-07-14 0952 K. Keenan Fed - Ex 1.5 9800 140507.09 Statesville - Performance Fibers NC__
05-07-14 0952 K. Keenan Fed - Ex 0.3 9801 140507.10 Raleigh CC NC 05-07-14 0952 K. Keenan Fed - Ex 2.8 9802 140507.11 Enviro Chemists - Roseboro WWTP NC 05-07-14 0952 K. Keenan Fed - Ex 1.0 9803 140507.12 United Water-Scotland Neck WWTP NC 05-07-14 0952 K. Keenan Fed - Ex 2.0 9804 140507.13 Wilson WWTP NC 05-07-14 0952 K. Keenan Fed - Ex 1.2 9805 140507.14 Statesville - Tuckertown WTP NC 05-07-14 0952 K. Keenan Fed - Ex 0.5 9806 140507.15 Elementis NC 05-07-14 0952 K. Keenan Fed -'Ex 1.3 9807 140507.16 Duke Energy - McGulre NS 001 N4C.
05-07-14 0952 K. Keenan Fed - Ex 2.8 9808 140507.17 Enviro Chemists-Bladen Bluffs NC 05-07-14 0952 K. Keenan Fed - Ex 2.8 9809 140507.18 Enviro Chemists-Invista - 001 NC 05-07-14 0952 K. Keenan Fed - Ex 2.8 9810 140507.19 Envlro Chemists - Invista - 002 NC 05-07-14 0952 K. Keenan Fed - Ex 0.4 9811 140507.20 Envlro Chemists - Manteo WWTP NC 05-07-14 0952 K. Keenan Fed - Ex 0.4 9812 140507.21 Duke Energy - Mayo Electric Steam NC 05-07-14 0952 K. Keenan Fed - Ex 0.4 9813 140507.22 Duke Energy - Shearon Harris NC 05-07-14 0952 K. Keenan Fed - Ex 0.8 9814 140507.23 Cameron Testing - Woodlake NC 05-07-14 0952 K. Keenan Fed - Ex 0.5 9815 140507 24 Duke Energy-McGuire NS 002 NC 05-07-14 0952 K. Keenan Fed - Ex 0.9 9816 140507.25
'PCS Phosphate, Incr-NC 05-07-14 0952 K. Keenan Fed - Ex 2.8 9817 140507.26 Carolina Beach WWTP NC 05-07-14 0952 K. Keenan Fed - Ex NA 9818 140507.27 Michelin - NMP Bath SC 05-07-14 0952 K. Keenan Fed - Ex NA 9818 140507.28 Michelin - Expoxy Bath
_SC 05-07-14 0952 K. Keenan Fed - Ex NA 9818 140507.29 Michelin - RFL Bath SC 05-07-14 0952 K. Keenan Fed - Ex 1.0 9819 140507.30 TVA - Shawnee Fossil Plant - Outall 001 AA' KY 05-07-14 0952 K. Keenan Fed - Ex 0.4 9819 140507.31 TVA - Shawnee Fossil Plant - Intake AA KY 05-07-14 0952 K. Keenan Fed - Ex 1.3 9819 140507.32 TVA - Shawnee Fossil Plant - Outfall 001 AP KY 05-07-14 0952 K. Keenan Fed - Ex 0.7 9819 140507.33 TVA - Shawnee Fossil Plant - Intake AP KY 05-07-14
.1000 K. Keenan UPS 0.4 9820 140507-34 Microbac - JP Stevens - Wagram WWTP NC 05-07-14 1000 K. Keenan UPS 0.4 9821 140507 _35 Microbac - Rockingham WWTP NC 05-07-14 1000 K. Keenan UPS 0.9 9822 140507.36 South Cary WW`TP NC_.,
05-07-14 1220 K. Keenan Dash Courier 2.3 9823 140507.37 OWASA NC 05-07-14 1200 K. Keenan J..Kennedy 0.5 9772 140507.38 Waynesville 'WWTP - Pp NC 05-07-14 1220 J. Sumner TVA Courier 0.710A 9773 140507.39 WA - Sequoyah Nudear Plant - 101-2 TN 05-07-14 1220
- 1. Sumner TVA Courier
. 0.3 9773 140507.40 WA - Sequoyah Nuclear Plant - Intake -2 TN2 Page 29 of 117 SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page_ 1 of 1
Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify):
I-&
)
eC -lv C 1y Facility Sampled: Sequoyah NP 28801.
General Comments:
_______________________ObK S0,4a ravjiiLC.
&&V?48r 0 01rv Cr~
ON C.~'1-944 NPDES Number: TN0026450 Phone:
828-350-9364 kr :Co
.n. ";-
-^t-L, t
a 3ezi,- '
Collected By:
Fax:
828-350-9368
'~~ t~~b ~
Container f~~~qw Field Identification I Number &
Flow Rain Event?.
Sample Description Grab/Cmp.
Collection DateiTime Volume (MGD)
(
Laboratory Use Collected (Mark as Appropriate) d*
Ye ETS Log Arrival Temp.
By Time Appear-i start End Yes
" fYes, No Trace MLoTieAp-Yes Inches Number o
t anoe SQN-I01-TOX-Z.
Comp b2(2.gal)
I'Zll.07 X
14 2.0 1
SQN-INT-TOX.3 Comp S 9t/
(2.5 gal) 21'"
T Sample Custody - Fill in From Top Down MV4A W'AS,tWACr.T SArjt9L.
Z 1 I Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time s
ft.- j
,..J-t/y
_0_a_66 f*.0-y
/Ao1TrI,.-L7q0f Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 3U Of 171
@11gS
.Page (Ao Whole Effluent Sample Receipt Log
- Sample temperature perfoimed using Sample Receivng Thernometer SN 130580151 Date Time Received Received
- Sample Project Sample Received Received by from Temp. CC) number number.-
y r nt Te t u be iu m b rSam ple flam e and description 'state Com m ents 05-09-14 0952 K. Keenan Fed - Ex 1.7 9792 140509.01 CORR ERI - AAF McQuay International NC 05-09-14 0952 K. Keenan Fed - Ex 1.4 9793 140509.02 Duke Energy - Belews Creek SS NC 05-09-14 0952 K. Keenan Fed - Ex 2.1 9794 140509.03 Enviro Chemists - Bladenboro WWTP NC 05-09-14 0952 K. Keenan Fed - Ex 2.8 9795 140509.04 Brunswick County WTP NC 05-09-14 0952 K. Keenan Fed - Ex 1.2 9796 140509.05 Statesvllle - Sparks Road WTP NC 05-09-14 0952 K. Keenan Fed - Ex 1.6 9797 140509.06 United Water - Enfield WWTP NC 05-09-14 0952 K. Keenan Fed - Ex 1.7 9798 140509.07 Duke Energy - Marshall SS NC 05-09-14 0952 K. Keenan Fed - Ex 1.2 9799 140509.08 Duke Energy - McGulre NS - 005 NC 05-09-14 0952 K. Keenan Fed - Ex 1.3 9800 140509.09 Statesville-Performance Fibers NC 05-09-14 0952 K. Keenan Fed - Ex 0.7 9801 140509.10 Raleigh CC NC 05-09-14 0952 K. Keenan Fed - Ex 2.1 9802 140509.11 Enviro Chemists - Roseboro WWTP NC 05-09-14 0952 K. Keenan Fed - Ex 1.6 9803 140509.12 United Water - Scotland Neck WWTP NC 05-09-14 0952 K. Keenan Fed - EX 1.0 9804 140509.13 Wilson WWTP NC 05-09-14 0952 K. Keenan Fed - Ex 13 9805 1405091.14 Statesvllle - Tuckertown WTP NC 05-09-14 0952 K. Keenan Fed - Ex 1.1 9806 140509.15 Elementls NC....
05-09-14 0952 K. Keenan UPS 0.6 9820 140509.16 Microbac -JP Stevens -Wagram WWTP NC 05-09-14 0952 K. Keenan UPS 0.6 9821 140509.17 Microbacs - Rockingham WWTP NC 05-09-14 0952 K. Keenan UPS 1.0 9822 140509.18 South Cary WWTP NC 05-09-14 1040 K. Keenan J. Kennedy 1.4 9772 140509.19 Waynesville WWTP - Pp NC 05-09-14 1228 K. Keenan Dash Courier 4.2 9823 140509.20 OWASA NC 05-09-14 1240 J. Sumner WA Courier 2.0/2.0 9773 140509.21 TVA - Sequoyah Nuclear Plant - 101 - 3 TN 05-09-14 1240 J. Sumner TVA Courier 1.8 9773 140509.22 TVA - Sequoyah Nuclear Plant - Intake - 3 TN Page 31 of 117 SOP G4 - Exhibit G4.2, revision 01-03-12
0ETS Page l of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seq uoyah Nuclear Plant NPDES #:
+/-L Project #:
County: -Rhea' AM 0'-
Outfall: 101 Dilution p Information:.
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080
'2160 "
2500 Diluent volume (mL) 2230 1960 1420 340 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test Information:
Organism age:
A.67-m- W t
- 2. Aot, 0d[
Randomizing template:
Aeb-q Date and times organisms osO.S.,4 o
-to %10O O
Incubator number and were born between:
shelf location:
I Organism source:
p frr.
A-rc.* Pp ogesi Artemia CHM number:
flii Drying information for weight determination:
Transfer bowl information:
pH= "1. &,S S.U.
Date / Time in oven:
___-1__
Temperature = V"A-
°C Initial oven temperature:
' L Average transfer volume:
Date / Time out of oven:
r-1 Final oven temperature:
Total f*edmne:."rnw ifom Daily feeding and renewal information:
14 00 Day Date Morning feeding Aflerpoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analst Outfall 101 Intake 0
OS.o-0e'
_ "4
",00 A
%13"1 1 bsos.07-Vo s.o-3 Nt, kc-2 A..
L.. It MO*M*
S*.....
O*.-
CA 0o 0 L.
1 t00 JA Ik~
q~ or
'4n-.
b'i 1*'4oJ~o.'4 os,-oeaqA1 4
ri-
-s 10 i---
-z--
V1-1,4066.
6 ri. R in 1Nib
-it N &0
-2 Aoscq.1.1 06ibr+
7 1 0%. 1-&. Iq
Tý I
U
- S'-
V-ILd I=
I-
% Mortality:
O1.
Average weight per initial larvae:
o."I4 Average weight per surviving larvae:
1 0.'19 eý20%
2t 0.25mgiAervae 7-day LC-o
> 107.
NOEC 1
LOEC
>z*?.
~ý 1007-.
ChV Page 32 of 117 IC25 I
)q 7.
SOP AT20 - Exhibit AT20.3, revision 07-01-12
JETS
'I-Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101. Non-treated Date:
OS.U0.A-ti Survival and Growth Data Day CONTROL 10.8%
21.6%
A B
C D
E F
G H
I J
K L
1 0
/0 0
/0 D
1/0 10 10 10 0O 1 0
/0tO t
to
/0
/0*
10
/0
/0
/0 to 2
io tO
/a 101/toC)0
/0
/010 3__
£C(C 1
I
() I C)
I
) I1 10 1 Io to 1()
to 4_to__
IO tO/
I t0 1
0 10 10 10-(/0 5
1 to(
10 10 0o (O
t' C) to ). 10 to 6
riIbI 0 1o~
lo I1 11 1 '0 to I0 7
Io to(QI 10 ID (t)
M1 [0 /b 10
/0 A - Pan weight (ag)
Tray color code::
Analyst: 04.)3 1
i F4 10.0"3 15.1, I.
lb.qo I03 16.55 6.13 I.
14 A
6 Date:
B P Pan + Larvae weight (mng)
Analyst:
kA Date:
W___.'_,_
____2_
O
'I...
p
.a:
g'tl 2.Oj
.b n.'t. 04S.(I~t C = Larvae weight (mg) = B - A AHndealyula1te."
- 1.
4
- 91.
4i.0ja 1.1 4
A.0 Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculate4.l Analyst:
N1 N
N 0
0*
0%
I,.
C 0%
Ia qW i3ýh 0it
_V3'110 V
rbs V*
1%
N N
V Average weight per Percent reduction Initial number of from control (%) I i
~ -
k b
I -
I o0.10
- AS7.
- 0. &%&
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
dt b~..by SOP AT20 - Exhibit AT20.3, revision 07-01-12 Page 33 of 117
1ETS Page 3 of 6 Species: Pbnephales promelas Client: TVA / Sequoyah Nuclear Plant, Oufall 101, Non-treated Date:
os-*Ok-54q Survival and Growth Data Day 43.2%
86.4%
100%
M N
.0.
P
,Q R
S T
U V
W X
10 /0 D 10/0 1
10 0
t
/
/0 0 /0
/0
)10 1 ID 10 10 It
'0 10
/
0 1
t) 10 1(
3 D
It) 0 010 10 0 10 1 10 1 IOD 0
lb to. to to
/0 toto Va3 to 0
- to to flO 5________
10 1)10 D
full(0
- 10)
()
I
't
'T 0 103 (Q 6
0
'0 to 10 to IC
()
q*
0 "0
0 7
10t O
- 0 IO C)
/
O t{
6o o
't 0 Io to. t
'o '0 A - Pan weight (mg)
Tray color code::
mA&uou it.o.
Ib.J 16.5Z ib.o I. i'i Ib.o1 6.t 1b.6 I.iq5 Analyst:
kew1.A R
t.C 6311.3IOO S4W0
%1MA Date:
0" 1
.1"I B=Pan Larye weight ln)
Analyst:
Al.7 l
.,a Date:
9.09 7"10 S4' "S'i Z-33 C = Larvae weight (mg)= B - A Hand calculated.
9113bt S.ka it-lb &At A.is S.M.*
'AAt 4.44 Ot.
0-3 ',MP
&.4%*
S.q Analyst:
n beolra(g Weight per initial number of larvae (rag)
C / Initial number of larvae b
V Hand-calculated.
J Analyst
~
~
~
.~
- ~*~~
Average weight per Percent reduction intial numbereo fromcontrol(%)
O.&HO t*4M'.-4 Q.$
I.-
- 7.
OOA.Va "O.07I Comment codes: c = clear, d = dead, fg = fingus, k = killed, m= missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
I F
I Comnwnis:
Page 34 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
ETS Page 4 of 6 Species: Ptmeyhales ro~mels Client: TVA / Sequoyah Nuclear Plant, Outfail 101, Non-treated Date:
1L Day 100% Intake
[ AA B
0 t o
/ 0 i D 1
2 3
tb
(
45 4 -
6 5 %
A C
(
7 I
A =Pon weight (mag)
Tray color coden::
mj~%
Date:
0q2-'l"-11A B =Pa + Larj -wegtMrg Analyst. _I eibt(g.
Date:
QS-1 C = Larvae weight (rag) - B - A Hand calculated.
3."1.
t1.28 1.0s Analyst:
(A ---
ý I
Weight per initial number of larvae (rag)
=C Initial number of larvae Hand calculated.
Average weight per Percent reduction initial aumber of from control o%) 1661 q
A
.7.
T a
ýr-t T
- - - I, - --
-I Comment codes: c = clear, d = dead, fg = fumgus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w -- wounded.
eOp$ 11iL
- PATHOMM~JC. I,4FeMtoN.
m 1435 L KUof11 Page 35 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 06-13, 2014 Pimq~halespronidas Chronic Whole Emuent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Proje number 9773
______Not for Compolianice Assessment.
Intenal
- m.
Laboraory_
CamnftAm (%)
R.pHkd.
S.1.mlarmd ibM-wlaedIsmwv Apiewnd&Im)
B-Pn+Larns L
e weasbt(ml* Wdiat/Serdkua Man.wdeitkI CaIe-boitalrvz*R"o WaghtlIMstoaw-bwe Ka-wmStl mmiaotlftu Caniwaor pemwe, ton rfm isor..
~b ewd (d
-A-B aa-boflnm(.,
0 S(at) 8 nag.
5*-I"M
.'.(l (V.)
-bhdhkfn.k
-kdoa G&..k maw (%)
A 10 10 14,35 22.07 7.12 0112 0.712 e
10 10 16.37 24.31 7.94 0.794 0.754 0.0 100.0 0.754 6.0 Not applieable Cotl C
10 10 16.29 23.69 7.59 0.759 0.759 D
10 10 16.03 22.92 6.89 0.689 0.689 E
10 10 15.13 25.00 9.87 0.987 0.987 F
10 10 16.31 25.00 8.69 0.869 0.901 7.0 0.869 100.
0%901 7.0
-19.5 10.8%
G 10 to 16.40 24.81 8.41 0.841 0.841 H
10 10 16.03 25.09 9.06 0.906 0.906 0,906 1
10 10 16.55 2.5.93 9.38 0.93, 0.93S 21.6%
J 10 10 15.73 23.50 7.77 0.777 0.898 9.4 777100.0 0898 9.4
-19.2 K
10 10 16.36 25.46 9.10 0.910 040.910 L
10 10 15.96 25.64 9.68 0.968 0.968 H
10 10 14A0 23.50 9.10 0.910 0.910 N
1 10
,15.93 74.21 8.28 0.928 0.940 14.2 0.828 100.0 0.940 14.2
-24.7 43.2%
N 10 10 16.04 27.37 181.33 1.133 11.133 P
10 10 15.32 24.20 8.88 0.888 0.888 1 10 t0 16A43 25.7*_
9.35 0.935 0.935 St6%
R 10 10 16.08 25.00 8.92 0.892 0.935 3.3 0.892 100.0 R.935 3.3
-24.1 S
10 10 15.74 25.39 9.65 0.965 0.965 T
10 10 16.02 25.50 9.48 0.948 0.948 U
10 10 15.86 25.09 9.23 0.923 0.923 100%
V 10 10 16.68 26.54 9.86 0.996 0.912 6,6 0.996 100
".912 6.6
-21.0 W
10 10 16.39 25.35 8.96 0.896 0.896 X
10 10 15.91 24.33 8.42 0.842 0.842 Y
10 5
1S.91 19.55 3.64 0.728 0364 Z
10 9
15.96 23.24 7.28 0.809 0.761 5.9 0.728 85.0 0.656 30.2 12.9 AA 10 10 16.58 23.86 7.28 0.728 0.728 BB 10 10 16.18 24.23 8.05 0.805 0.805 Doorettis 51191 valve:
Intakat.
Doneuadl HME vuuet PMSD.h 0.1311 11A7 0.1975 26.2 MSD -
Minimum Significant Diffemrce PMS? -
Percent Mimum Significant Differfnce PMSD is a measure of test precujon. The PMSD is the minimum percent difference beteesm the control and tnrtment that cma be declared statistically signifiamat in a whole effluent toticity test Lower PMSD bouid dclermnined by USEPA (I 0th percentile) - 12/.
Upper PMSD bound determined by USEPA (90th percewtile) - 30%.
Lowme and upper PMSD bounds were determtned from the 10th and 90th percentile. respectively, of PMSD dea from EPA's WET Intarlaboulory Variability Study (USEPA, 2001a, USEPA, 200 1b).
USEPA. 2001a, 2001b. Finra Report e rnhortaqy Variability Study of`EPA Shot-lertm Chronic and Acute Whole Effluent Toxdcity Test Methods, Volumes I and 2-Appedix, EPA-821-B-01-004 and EPA-821-8-01-005. US Environmental Protolion Agency, Cincinnati, O.
Page 36 of 117 File: s*0l1_OSO614dat*asx Entered by: J. -Vnn Reviwed by:
TVA / Sequoyah Nuclear Plant, Ouffall 101 Non-treated May 06-13,2014 S
6 SSETS Statistical Analyses
- Larval Fish Growth and gurvival Taet-7 Day Growth Start Date:
5/1/2014 Test ID: PpFRCR Sample ID:
TVAI SQN, Outrall 101 End Date:
5113/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Dlscharge Monitorlng Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species; PP-Pimephales promeles Comments:
Conc-%
1 2
3 4
Non.Control 0.7720 0.7940 0.7590 0.6890 10.8 0.9570 0.5890 0.8410 0.9060 21.8 0.9380 0.7770 0.9100 0.9880 43.2 0.9100 0.8280 1.1330 0.8880 86.4 0.9350 0.8920 0.9650 0.9480 100 0.9230 0.9860 0.89(10 0.0420 Intake 0.3840 0.7280 0.7280 0o.050 Transfoea: Untrensformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Cdtical M8D Mean N-Mean Non-Control 0.7535 1.0000 0.7535 0.6890 0.7W40 0.020 4
0.8896 1.0000 10.0 0.9008 1.1954 0.9008 0.8410 0.90870 7.035 4
-2.706 2.410 0.1311 0.8898 1.0000 21.6 0.8983 1.1921 0.0983 0.7770 0.9880 9.377 4
-2.660 2.410 0.1311 0.8898 1.0000 43.2 0.9398 1.2472 0.9398 0.8280 1.1330 14.197 4
-3.423 2.410 0.1311 0.8098 1.0000 10.4 0.9350 1.2409 0.9350 0.8920 0.9850 3.336 4
-3.330 2.410 0.1311 0.8898 1.0000 100 0.9118 1.2100 0.9118 0.8420 0.9860 6.588 4
-2.909 2.410 0.1311 0.8890 1.0000 Intake 0.8583 0.709 0.6563 0.3840 0,8050 30.200 4
Auxiliary Teat-Statlatic Critical Skew Kurt Shapiro-Wilk's Test Indicates normal d&ribution, (p > 0.01) 0.95715 0.884 0.87813 1.88971 Bartlett's Test indicates equal variances (p = 0.20) 0.54*43 15.0863 Hypothesis Test (114al, 0.03)
NOEC LOEC ChIV TU MeDU MSDp MIB MWS F-Prob df Dunnetts Test 100
>100 1
0.13112 0.17402 0.01903 0.00592 0.03013 5, 18 Treatments vs Non-Control Unear Interpolation (200 Resamplea)
Point SD 98% CLAExp)
Skew IC05
>100 IC10
>100 IC15
>100 1.0 IC20
>100 0.9 iC25
>100 0.8 IC40
>100 0.7 IC50
>100 0.e
.04 0.3 0.2 0: 0.1 0.0
-0.12-
-0.3
-0.4 a
80 100 ISO Dose %
Dose-Response Plot 1.2 10.8 0.4
,- 0.4 0.2-1-tall, 0.05 level of significance 0,
4 CR C'4ý0 o
~
t COi Pa,_
by Page 37 of 117 File: sqelOl05061 4dAt.xlsx Brituredb :J Reviwl by:h
TVA / Sequoyah Nuclear Plant, Intake Non-treated May 06-13, 2014
& vr~onniintalTsst
.Ulng S kaImus u.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:
516/2014 Test ID:
PpFRCR Sample ID:
TVA/SQN, Intake End Date:
5113/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-%
1 2
3 4
Non-Control 1.0000 1.0000 1.0000 1.0000 10.8 1.0000 1.0000 1.0000 1.0000 21.6 1.0000 1.0000 1.0000 1.0000 43.2 1.0000 1.0000 1.0000 1.0000 86.4 1.0000 1.0000 1.0000 1.0000 100 1.0000 1.0000 1.0000 1.0000 Intake 0.5000 0.9000 1.0000 1.0000 Transform: Arcsin Square Root 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Non-Control 1.0000 1.0000 1.4120 1.4120 1,4120 0.000.
4 10.8 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
21.6 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
43.2 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
86.4 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
100 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
Intake 0.8500 0.8500 1.2146 0.7854 1.4120 24.393 4
1.332 2.353 0.3486 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.76106 0.749
-1.6615 4.05792 Equality of variance cannot be confirmed Hmotheels Test (1-tall, 0.05)
MSDu MSDp MSB MISE F-Prob df Heteroscedastic t Test indicates no significant differences 0.21111 0.21663 0.07793 0.04389 0.23107
- 1,6 Treatments vs Non-Control Dose-Response Plot 14 0.9 0.8 0.7 0.6 0.5 9*0.4:
0.3 0.2 0.1 p
p 1-tail, 0.05 level of significance 2
q C
't a
CM CD N
~.
L Page 38 of 117 File: sqnlO1_0506]4dat&xsx Entered by: J. Suina Reviewed by:
TVA / Sequoyah Nuclear Plant, Intake Non-treated May 06-13, 2014
- ET Ez ivmrnmugdal TowneSoludoni. Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
516r2014 Test IDr PpFRCR Sample ID:
5/13/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pirnephales promelas Comments:
Conc-%
1 2
3 4
Non-Control 0.7720 0.7940 0.7590 0.6890 10.8 0.9870 0.8690 0.8410 0.9060 21.6 0.9380 0.7770 0.9100 0.9680 43.2 0.9100 0.8280 1.1330 0.8850 86.4 0.9350 0.8920 0.9650 0.9480 100 0.9230 0.9860 0.8960 0.8420 Intake 0.3640 0.7280 0.7280 0.8050 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Non-Control 0.7535 1.0000 0.7535 0.6890 0.7940 6.020 4
10.8 0.9008 1.1954 0.9008 0.8410 0.9870 7.035 4
21.6 0.8983 1.1921 0.8983 0.7770 0.9680 9.377 4
43.2 0.9398 1.2472 0.9398 0.8280 1.1330 14.197 4
86.4 0.9350 1.2409 0.9350 0.8920 0.9650 3.336 4
100 0.9118 1.2100 0.9118 0.8420 0.9860 6.686 4
Intake 0.6563 0.8709 0.6563 0.3640 0.8050 30.200 4
0.957 1.943 0.1975 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.8402 0.749
-1.7067 3.68449 F-Test indicates equal variances (p = 0.04) 19.0884 47.4683 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.19753 0.26216 0.01892 0.02067 0.37568 1,6 Treatments vs Non-Control Dose-Response Plot 1.2 0.8 0.6 0 0.4 0.2 1-tall, 0.05 level of significance File: sqnlOl_OSO614dM.xlsx Entered by: J. Sipnnr Reviewed by:
Page 39 of 117
aETS 6 ft---V..,*
-L Page 5 of 6 Species: pimebhakes promdas Client: TVA / Senuovah Nuclear Plant. OutfaH 101. Non-trated Daly Chemistry:
Date:
os 0--1 IDay (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) I Analyst I tW I
M 1
I 2
I Concentration IParameter PIK (S.U.)
Conductivity (irnhos/cmi)
CONTROL Non-treated 9
- Aibilialty 1(MR CaC0iJL)
- Hlardness (MAi CaCOIL)
- Temperature (Oca
'U U
'U oH (S.U.)
DO rmclL) 10.8%
Conductivity (umhos/cmn)
- Temperature (CC oH (S.U.)
DO (mg/L) 21.6%
Conductivity (umhos/om)
- Temnerature (C0 PH (S.U.)
SI M-8 11 1.4L-I
~ 1
~
LH DO (mg4L_
43.2%
Conductivity (uinhos/oin)
- Temnerature (0C)
PH (S.U.)
DO (m*,.L 86.4%
Conductivity (umhos/cm)
- Temperature ('C)
DH (S.U.)
DO (mg/L)
Conductivity (mrhos/cm) 100%
- Alkalinity (Ing CaCO3/L)
- Hardness (rngCaCO3/L)
- TR chlorlne (ma/L)
-*Temnerature-(O.-
PH (S.U.)
DO (mizIL) i L~~wnosim) 07O' IC Conductivity
- Hardness I
(Mg Caco A-)
,R chlorne (mg/L)
Temperature
.*C 7A -I-S.,
0 1" ~
V. 90.
Initial FInal Initial Final Initial Final eratures performed at the time of test initiation, renewal or termaination by the analyst identified in the Daily Renewal Information table locted on Page 1.
Pa.MW ity, hardness and total. residual chlorine performed y the analyst idenified on the bench sheet specific for each analysis and transcribed to this bench sheet Page 40 of 117 SOP KI20 - Exbibit AT2O.3, revision 07-01-12
Page 6 of 6 Species: Pbneghala Dromelas Client. TVA / Seauovah Nuclear Plant Outfall 101. Non-treated Date:
cS~.fl -04 Ia I
I Day (Analyst identifed for each day. verfonrmd PH. D.O. and conductvity measurements only.)
I m
I-m 3
I 4
I
.5 I
6 Analyst I'W Concen-Parameter traton i
-PH (S.U.)
DO (mg/L).
Conductivity CONTROL Non-treated
- Alkalnlty (ing Caco3 /L) 6.0
- Hardoeas (Mg~aO/)
MLU M 1:
M.~
- TemDerature 00'C a oH[ (S.U.)
PH
.-8U--"
DO (ma/L) 10.8%
Conductivity I2v (gmhos/cm) 1
- Temnaratnre &C pI* (S.U.)
FDO (nir/Ll 1(o 21.6%
DO
_____i~~<Q tTam nm'ahlre I
Ilq.
-t*.
- I
-. d "2 I l~.'t
- Temperature nlH] (S.U.'t r--
..__=
DO (mnL) 4- -~
43.2%
Conductivity (ttmhos/cm)
- Temnartn~ (~C~
-Temp---t e
,0_
oH (S.U.)
DO (Mg/L) 86.4%
Conductivity I/unMmh0.q/
- Temuerature C Ir
]pH(S.U.)
DO (MM/L) tom Conduictivity
- Alkalinity (ing CaCO3/L)
HEardness (Mg CCOMAI)
- Th chlorine (mgi'L
- Temveratre (0C)
Intake pH (S.U.)
DO (mWI)
Conductivity (Lu C-ac3/)
- Hardness (Mg CaICOce()
'TR chlorine (,ng/L) i Initial 11 Final I
Initial FInal I
Initial1 Final hn~tI'WIal
-FIn'al eratures performed at the time of test initiation, renewal or tennination by the analyst identified in the Daily Renewal nomatioo table located on Page 1.
Akall ity hardness and total residual chlorine perforned by the analyst identified on the bench sheet Wecific for each analysis and transcribed to this bench shed P5 ge 41 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
a Evlronmuntal Testing Solutions, Inc-TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 06-13, 2014 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number 9773 Day I
V T
~
I V
T Concentration IParameter Day I
lay I
I I
Day Dav 4 1
Day I
If.IH, Initial I
Final I
Initial I
Final I Initial I
Final I
Initial I
Final hi I
Final I Initial Final 9-pH(w~
7.81' 7.991 7.561 798 7591 7.91 17.71 7711 7.991 7_.5 h -
4-
__4
-b
-4 Control DO tnMlL) 4-Ir-A
+;.i h-J.-
n i
M U U=
I Alkalinity (mgAL CaCO3 )
I 7.7 300 61 86 7.8 302 62 86 7.7 302 60 94
- 7. 6 306 62 88 7.7 310 58 92 T7-Hardness (mglL CaCO3)
I Temperatare (C.
24.9
.1 24 24.5 24.8 24.6 24.8 243 248 246 247 246 24.8 24.5 1H ISU) 8.04 779 8.01 7.57 7.88 7.55 7.95 7.66 797 772 799 759 8.00 7.69 DO (8o-L) 8.1 80 79 7.3 7.8 7.6 7.9 76 7.8 75 78 73 7.9 7* 5 10.8%
Conduetivik (inmhoufm) 305_
295 313 3
3 7 301 309 Temperature CC) 25.0 24.6 24.8 24.8 24.8 24.3 24.8 24.5 24.8 24.6 247 242 24.9 24.6 pH (STM 8.05 71 8.01 7.58 8.06 7.53 7.95 7.68 7.98 7.71 800 756 8.01 7.69 DO (m./L) 8.2 80 79 72 7.8 7.6 7.9 7.5 7.8 75 79 73 7.9 7.6 21.6%
Conductivity (nmholcm) 287 28241 1 303
-27 289 2&2 293
- 4 Temperature (iC) 25.0 24.5 24.8 24.4 24.8 24.5 24.8 24.5 24.8 2,45 24.7 247 24.9 24.6 pH (SUb 8.05 7.84 8.02 7.64 8.07 7.52 7.94 7.72 7.98 770 796 757 8.01 7.68 DO (MAD/L 8.2 7.9 7.9 7.2 7.9 7.6 7.9 7.5 7,8 75 80 72 7.8 7.6 43.2%
Conductivity (ioser) 258 254 298 2
257 2.0 25 I:
Temperature (UCP 25.0 24.7 24.8 24.7 24.8 24.5 24.8 24.8 245 247 246 25.0 24.6 PH (Su) 8.05 7.851
.02 7.60 8.07 7.52 7.95 77.97 771 797 757 8.00 7.68 4 O (IIIAL) 8.2 7.9 7.9 7.1 7.9 7.6 8.0 77.7 74 79 72 7.9 7
86C4%
Conductivity (wmhosl/em) 203 198 221 9 0 190 M
Temperature _
25.0 24.4 24.9 243 24.8 24.5 24.8 24A 24.8 2477 244 25.0 24.7 PH (S_
8.07 7.86 8.02 7.62 8.07 7.52 7.95 7.70 7.97 7.991 7.57 8.00 7.68
]DO (ywL) 8.2 7.9 7.9 7.1 7.9 7.6 8.0 7.5 7.7 78.0 7.2 7.9 7.61 Coduentivity (nughos/em) 183 176 188167 174
.0168n 100%
Alkaliniy (mw/L C&CO3) 65 65 67 flardaess (mgWL CaCO 72 78 64 Total Rtesidual Chlorine (mplL)
<0.10
<0.10
<.10 Temperature (90 25.0 24.9 24.9 224.8 24.5 25.0 24.9 25.0 24.6 PH (S.
8.10 8.04 78.05
. 8.02 7.8 8DO
(._L 82 78.0 7.11 7.9 8.1 7.7 7.9 7.9 7.5 Conductivity (Mnnhos/cm) 200 183 207M M
180
.199 17 179 100% Intake Alkalinity (mg/L CaCO3) 67 65 67 Hardness (mg/]L CaCO1) 74 68 70 Total Residual Chloria (ne /L)
<0.10
<0.10 Temperature (C) 24.9 24.6 25.0 24.6 24.1 24.6 24.9 24.6 25.0 24.6 24.7 24.3 25.0 24.7 Page 42 of 117 File: sqn101_050613che'n.xfsx Entered by: J. Sumner Reviewed by:
- ET b
%"*fft Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Seguoyah Nuclear Plant NPDES #: TN0026450 Project #: _
_r_
County: Hamilton Outfall: 101 Dilution preparation information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information:
Test information:
Organism up_:
I< 24-hours old Randomizing template color Date and times orgims w M
Incubator number and shelf location:
Replicate number:
I1 2 1 3 14 5 1 6 1 7 YW batch:9 1
CAultureboard voume m': 1 1AlU hl 1 5 1 '1 13 1batch:
,0 LAve-ragetransfervolume(mL): I O. ORJ-..v..
I
./nsrmbth Daily renewal information:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outffll 101 Intake termination time
- 0.
06_6__
o4..t.C 4
"oS.o.IL "
4.b
,Lb 2
Oal'.
o08-6nA stQOSU
'%t Iqo -%Owl.,1 5
os.ii-A 6
o08+
L4JSq-L1I L-AOSOq-L-L 7
-[;:
1 bog Contrl inormaion:Summary of tes t endpoinLa:
i Control-I Control-2 Acceptance criteria
% of Male Adults:
0
?,
6 20%0 7-day LC5 0
> 1001.
% Adults having 3' Broods:
- 1067, 1061.
2 80%
NOEC t 07.
% Mor*aty:
A7,
- 01.
- 20%
LOEC
> I07.
Mean Offs 'qWFemale:
2P.A t.,
>15.0 offspdrgfmale ChV
)'1007.
% CV:__O_
<40.0 %
s
>Io07.
WY L b-Page 43 of 117 SOP ATI I - Exhibit ATI 1.2, revision 07-01-12
IETS 0 *.
70"Im.*
Page 2 of 7" Species: Cerodaphnli dubia Client: TVA / Seauoyab Nuclear Plant, Outfall 101 CONTROL-1
- Sam, Date: __byO__1@
iwal and Reproduction Data Replicate number Day -1 2
3 4
5 6
7 8
9 1
1 Young produced 0
Adult mortal...
_',,,,,~C fi I
I0 --
Adult mortality
'-I L..
L-.
k.-.
3
Young prod aced 0
C) 17 11%010 4
Young produced Adult mortaity L
-f-J~JL I..
Y Young produced C)
L "i 4
.Ž L
1 1
L
-Inal Adult mortality
].,
I I
I..
o *Bros*
=
=.
=
"-=
SYoung produced Adult mortr 7N 10 Younrproduvei[l ajd
%%k
%5 Data prd uyoung produced Adult mortality ToaYoung produsced i
-1.
FalAdultmort_
i 2
o? I IX for 3" Broods v
r Note., Adult mortailty (L hk Dw dead).
- spit braod (aistgle brood sprit btwwmt two days),
CO-carry over(offpttng carried ovar with adult duringtrawsstr).
I Concentidon:
%Morital 07J Mean OMi rin emale:
CONC: 10.8%
Survival and Reproduction Data
_____Replicate number Day 1__
1 2
3
- 4.
5 6
8 1
9 10
-dl motait L-L-
t-
-L 2
Young produced jAdult mortality 2
Young produced[
i Adult mortality
[="tt_=
oung produced 1.14
=a-..
6 Young produced r):
C)L C
_)C-Adult mortality t,-
\\-_j--
L-L 57 Young produced W-1%
1" Toa young produced
'b C) 3S 0L 3 1.l. S.L T"
00Bt Final Adult Mortality L
L.
e.
Note: Adult mmtgft CL - Im D - damdj6 3
A so bawpan two day2), CO - am, over (offigring =
ever with adult dwim tranglhr)I" Page 44 of 117 Cocenb&adon
% Mortality:
1Mean Offpring/eiale: -
% Reduction from Control-l:
S.7.
SOP ATI I - Exhibit ATI 12, revision 07-01-12
PETS
- "'W Page 3 of 7 Species: Cerlodawhnia d*bla Client: TVA / Sequoyah Nuclear Plant. Outfall 101 coNc: 21.6%
Suwv Date: ___adRpouoa ivuI and Reproduction Data Replicate number Day 1
2 3
4 5
6 71 8
9 10 I
Young produced 0O 0
0 1 00' Adult mortality
\\..
L L.
L..
L 2
Young produced c)-
0i I
=
Youn produ e 0
0 0
ŽI Adult mortality JL.
L.
L I.-L.
Y Young produced
)
[
0 C)
Adult mortality L
u
_. L..
-- I 5
Young produced Adl Moralitlity 6OC Young produced a6d Rer6uo Da)
Adult mortality I
\\_.\\_
7 Young produced nj Toa you~ngprofduee d%
A 1
A 0
F.1'i.
SAdultmortality
'_. IX.-
6s Young produced "i,
- f j
- .L, Final Adult Mortality k....
Note. Adakurtel (L-iM D-deed),SU-V bood (igle brood sph Womtwo days),CO - a vr o
(oftorin eandedo uww th d~t dwimt a W,
cow: 43.2%
Survival and~ Reprouctio Dai 1~nl Adult Mortali
- k-.-
W
?
Adult1 moortatylity:k Mea YoungrproduceaiIS I S to Adl mortalitoyCotrl-:
.7 (DC Y3.2%
produced and Re1du1o Dat 6
Young produced C)
C)
C0i Q) 0 l..
Adl mortality 1...
L j
L.
L.
JL.. J' I
Young prodluead I~
t) 01 1 0
0 0
1(
3oa young produced IS6~
.6 3.
IS. ate--
Fnl Adult Mortality U.
At 1 Adu
___k (L -_ UmD-___B-q ro doboo &btemtodzC v;ýý,
vrwhsu tn rnr I Conceniadoun:
I bkiE. by.
Page 45 of 117
% Mortality:
I o.
Mean OffspringfFemale:
l SA
% Reduction from Control-i1:
SOP AT 1I - Exhibit ATI 1.2, revision 07-01-12
di Species: Ceriodahnia-dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 coNc: 86.4%
Survival
[
Page 4 of 7 Date:
o&-lo"m "A and Reproduction Data 5
6 7
8 9
10
-- }
I Young produced 101 0
0 0
IAdult mortality j._
-II'I T......
V.
=
I.. =
=
=
2 Young produced 0
0 0
0 3oa Young produced 0******"'1 o.*
Fnl Adult mortalit y
.Adultmtft
-vDded,
=pbods boositbw todyO-a motliyVQ'o~iaueoew~hdld mse
_ Young pre
morta li=t-y
k L
6 Young produced O
.A 5
)
1 I
I)
I Adult mortality L.
-Fe
____I=
7 Young produced l...Ž..AL=
_Adult moortalityI....
L..
L
.. 1 L
Total young produced
-ha 57- :I pzý w"
6FidtalA M ortl i L,,
N*
urorlt LHDduad),SD - s~brood(ahir!u brood split btwmtwo days)CO -
,over (offorhuz cante owr with adult durasitnfa4)
%. Mortality:
W.
-Mean Offsring/Female:
I 304. S
% Reduction from Control-1:
- i.'7r CONC: 100%
Survival wtd Reproduedorn Data 1__epicatenumber_
C_
- __E0 C
__O_
Adult mortality C
q IL-L_
Yun prouce 11 2I__j 8 t_
L 21 Young produced C) 0
- 0)
- 0)
C)
C
)t
.0..Q.
Adult mortality T1
[
-41~
3
[Young produced 1)
~
~
Adult mortalitTy L
~...
4 Young produicedj
- s.
I I
II Adult mortality t..
-~
~
-I' 51 IYoung produced
%A 12~
T%
1 W
JAdult mortality I
6 Young produced L0~T C)-~
_ Adult mortalityl LtL.
U L ~ L..~
I..
L_
7 Young produced Total young produced 1Final Adult Mortality
\\_
V.-
I -
I T
C I. \\_
I.
NoM.: Adut momuty (L - I.
U-e D-d,)
B - a8pM 00ood (sb b0ood Spli betmen two dy), CO - am, over (of3fi m caned over *iah at durnm u
).
Concentradon:
% Mortality:
01.
Mean Offspring/Female:
% Reduction from Control-1:
.1.
Page 46 of 117 SOP AT1 I - Exhibit ATI 1.2, revision 07-01-12
MIETS Page 5 of 7 Species: codQa'Uhnia uhLa Client: TVA / Sequpyah Nuclear Plant, Outfall 101 Date:
O____t__
CONTROL-2 Survival and Reproduclion Data Replicate number Da 2
3 4
5 6
17 8
9 10 1
Young produced
(
C Adult mortlity
- t.
L.
Adult.mortality L -
- J -
I L......
.I "
3 Young produced b
C)
L C.
Adultmortality.
L.
I 4-'
I 4
Youngproduced lJ
,fl
, *fl f
Adult mortality L
L
.. Jl.
j j
-" I
=
tm ~ i I
45 Young produced Adult mortality L f 6
Y oung produced c)~
6 11 0
1
-)
0L jA 1
Adut mortality
'1 7~
Young produced I lb L-&
1Sa A
0 Total young produced 1A
'hi
!)
2S 16 SL
?2 Q
&0 3
I Fnal Adult Mortalityj-W X for 3' Broods y
SK.
74
.,oYe: Adu-lt monm y (L -mva, D-dd), SB - split brood(sinle bod soft betweentwo dayu), CO-*tyvoy(of*m h
Ie ow with adult dwin ttuk)
Concentralton:
/o.Mortality:[
1 Memi Offs emale:
- l.
- c Survival and Reproduction Data Cco.: 100% Intake
_ Replicate number Day 2
3 4
5 16 7
18 9
10 Young produced 0
0 Adult mortality L
2 Young produced
)0 0
4 3
Young produced 0
0 0
I C)
Adult mortality I
4 Young produced Vft~
A 5
Young produced
\\-
I-L 1
IL"..... i.
AyI-6 Young produced
.fl f"Q.
,fli n Adult mortality 71 Youngproduced II I1 Total young produced 1-'[ 3%
~
~
~
I ~
Final Adult Mortality
'S-W 5i
?I
~
~
£'
Nom.- Adult mmtally (L live, D -dead) 9B -sylit brood (do&~ broad spit betwa two days) CO - mm~
(ofa naaid uwihdtdri tek Concentration:
% Mortality:
07.
Mean Offspring/FFemale:
N*qi.
% Reduction from Control-2:
-".'7, SOP AT] I - Exhibit ATI 1.2, revision 07-01-12 Page 47 of 117
- ETS SEmm m lTd SSll*,Ic TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 06-13, 2014 Verification of Ceriodaphnia Reproduction Totals Control-I Rg Relicate number Total Day 1
2 3 14 5
6 17 18 19 110 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
5 4
4 6
4 6
6 4
48 I 1I 10 1
0 10 12 12-12 11 12 Hl!
6 0
0 0
0 0
0 0
0 0
0 0
7 16 14 14 15 14 12 13 12 15 15 140 Total 31 29 30 29 28 30 29 30 32 31 299 10.8%
Dal Replicate number Tot3 l Day 1
2 3
4 5
6
'7 8
9 10 1;
0 0
a1 0
0 0
0 0 a0 0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 41 6
4 5
5 4
5 5
- 5 4
47 5
11 13 11 12 12 11 10 13 12 10 115 6
0 0
0 0
0 0
0 0
0 0
0 7
17 16 17 17 17 1
1 15 1
7 17 165 Total 32 35 32 32 32 29 333 33
. 33 391 21.6%
DyReelicate numberToa D
1 2
3 4
5 6
7 8
9 1101Toa 1
0 0
0 0
0 0
0
+/--
0 a
0 2
1 0 D o f 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 5
4 6
4 5
5 5
4 6
6 50F 12 10 12 11 11 11 10 12 11 12 112 6
10 0
o 0
0 0
0 o0 0 0
o.
7 116 16 15 18 115 1 6 t16 13 6 15 FS56 Tojtalj 33 30 33 33 131 132 131 29 VL33 133 1 318 43.2%
DyRAeDlicate number
.Totl[
D y 1
- 2. 3 1 4 5
6 7 1 8 19 110 1 1
-2 0-0 0
0 0
0 10 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0-0 0
0 0
0--.0 0
4 6
5 I6 4
4 5
5 5
5 6
51 5
12 13 13 11 13 12 10 13_
13 13 123 6
10 0
0 0-0 0
0 0
0 0
0 7
181 14 16 17 17 16 18M1 15 17 17 111 ITotal 36 1 A 35 3.2 3.4 Z3 33 1D 35 1.361 339 86.46/
RE f number Total Day t
2 3 14 9
6 17 1$
9 1 0 1
0 0
0 0
- 0.
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 5
4 5
5 6
4L 6
6 15j 6
12 5
12 12 14 11 1 2 I
1 3 12 1ý3 14 124 6
0 0
0 0
0 0
0 0
0 0
0 7
16 16 19 16 18 19 15 19 19 15 172 Total 33 32 38 32 3
3 34 37 37 35 348 100%
DyReplicate number Total 1a 2
3 4
5 16 7 18 19 1 0 1
0 0
0 0
0 0
0 0
0 01 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4, 6
5 5
.4 5
6 5
-5 6
6 53.
5 12 14 13 12 13 13 13 14 14 1
3 6
0 0
0 0
0 0
0 0
0 0
0 7
19 19 18 19 15 17 16 17 15 18 173 Total 37 38 36 35 33 36 34 36 35 36 356 Control-2 Tay Re 2lic3te number Total 10%
1 2
3 4
5 6
7 8 19 10 1
0 0
0 0
0 0
0 0
0 0 a 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
5 4
4 6
4 4
4 5
4 44 5
11 1I 1 I0 12 10 12 12 12 11 12 113 6
0 0
0 0
0 0
0 0
0 0
0 T
33 13 1 3
31 3 1 7 1 6 1 4 3 3 1 4 31 4 4 Total [28L 29 130 130 129 133 32 30 30 130 1 301 100% Intake Re plicate numberI Day 1 12 3
4 5 16 7
8 19 10 Total 1
0 o 0 0
0 1 0 o
0 0
0 0
2 0 1 o 0 o
0 0
0 0
o D
Y3 0
0 0
0 0
0 0
0 0
0 0
L4 6
5 6
4 6
5 5
5 6
5 53 5
12 11 I
13 10 13 12 10 12 12 116 6
0 0
0 0
0 0
0 0
0 0
0 7
15 15 13 14 7
12 15 15 11 14 145 taTotal 33 31 3r 33 130 132 130 [33 31 314 1
Page 48 of 117 File: s~ri101_OSO6l4d~ataL~xf Entered by-..2. Sumn~er ReAewed by.-X
61 Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 06-13,2014 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
9713 Comcentrateo*
Repicate number Survival Average reproduction CodfftLmet of Paecent redurli-from 1
2 3
4 5
6 7
a 9
10
(%)
(offspring/female) vleu(%)
contro (%)
Control - 1 31 29 30 29 28 30 29 30 32 31 100 29.9 4.0 Not applicable 10.8%
32 35 32 32 32 29 30 31 33 30 100 31.6 5.4
-5.7 21.6%
33 30 33 33 31 32 31 29 33 33 100 31.8 4.6
-6.4 43.2%
36 32 35 32 34 33 33 33 35 36 100 33.9 4.5
-13.4 86.4%
33 32 38 32 36 34 34 37 37 35 100 34.8 6.2
-16.4 100%
37 38 36 35 33 36 34 36 35 36 100 35.6 4.0
-19.1 Centrol - 2 28 29 30 30 29 33 32 30 30 30 100 30.1 4.8 Not applicable 100% Intake 33 31 30 31 33 30 32 30 33 31 I00 31.4 4.0
-4.3 OtfIall 101:
Dunnett's MSD value:
PMSD:
Dunue's MSD value:
PMSD:
1.645 5.5 1.055 3OSS MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. Tbe PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA ( 0I percentile) = 13%.
Upper PMSD bound determined by USEPA (90& percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPAs WET Interlaboratoiy Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-933-R-00-003. US Environmental Protection Agency, Cincinnati, O.L USEPA. 2001a. 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OHR File: sqn1Ot_OSO614data.xlsx Table populated from associated "Verification of Ceriodaphnla Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Surgner Page 49 of 117 Reviewed by.O
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 06-13, 2014 a
S 0fTS bukouu.nuiTngSatnflc.h~
Statistical Analyses
.e nuuapnw Surviva an6 Mepruuucuon I eat-eprovuuuuon Start Date:
518/2014 Test ID: CdFRCR Sample ID:
TVA I SON, Outfall 101 End Date; 5/13/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
CD-Cealodaphnla dubla Comments:
Conc-%
1 2
3 4
6 6
7 8
9 10 Control-1 31.000 29.000 30.000 29.000 28.000 30.000 29.000 30.000 32.000 31.000 Control-2 28.000 28.000 30.000 30.000 29.000 33.000 32.000 30.000 30.000 30.000 10.8 32.000 35.000 32.000 32.000 32.000 29.000 30.000 31.000 33.000 30.000 21.6 33.000 30.000 33.000 33.000 31.000 32.000 31.000 22.000 33.000 33.000 43.2 36.000 32.000 36.000 32.000 34.000 33.000 33.000 33.000 35.000 36.000 88.4 33.000 32.000 38.000 32.000 36.000 34.000 34.000 37.000 37.000 35.000 100 37.000 38.000 36.000 38.000 33.000 38.000 34.000 36.000 35.000 38.000 Intake 33.000 31.000 30.000 31.000 33.000 30.000 32.000 30.000 33.000 31.000 Transform: Untranefrnned 1-Tailed Isotonic Canc-%
Mean N-Mean Mean MNn Max CV%
N t-Stat Critical MSD Mean N-Mean Control-I 29.900 0.9934 29.900 28.000 32.000 4.004 10 32.933 1.0000 Control-2 30.100 1.0000 30.100 28.000 33.000 4.814 10 10.8 31.800 1.0498 31.600 29.000 35.000 5.420 10
-2.383 2.287 1."45 32.933 1.0000 21.6 31.800 1.0565 31.000 29.000 33.000 4.641 10
-2.640 2.287 1.645 32.933 1.0000 43.2 33.000 1.1282 33.900 32.000 36.000 4.495 10
-5.559 2.287 1.645 32.933 1.0000 88.4 34.800 1.1581 34.800 32.000 38.000 8.178 10
-8.810 2.287 1.845 32.933 1.0000 100 38.800 1.1827 35.600 33.000 38.000 4.015 10
-7.921 2.287 1.648 32.933 1.0000 Intake 31.400 1.0432 31.400 30.000 33.000 4.028 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test Indicates normal distribution (p > 0.01) 0.75245 1.035 0.01981 a.8036 Bartletta Test Indicates equal variances (p = 0.63) 3.488W4 15.0853 The control means are not significantly different (p = 0.74) 0.33648 2.10092 Hypothesis Test (1-tall, 0.05)
NOEC LOEC CIV TU MSDu MSDp MSB MSE F-Prob df Dunnelt's Test 100
'100 1
1.64541 0.05503 47.5887 2.58889 1.3E-10 8,54 Treatments vs Control-I Linear Interpolation (200 Resamples)
Point SD 98% CL Skew ICos
>100 IC10
>-100 IC15
>100 1.0 IC20
>100 0.9 IC25
'100 0.5 IC40
>100 0.7 IC50
>100 0.8 0.8 0.41 0.3 0.2 0.1 0.0
-0.1
-0.2-
-0.3 1..
0 5O 100 150 Dose%
Dose-Reeponse Plot 40 35 30 2.5 S20 110 5.
111 jiý.....
I.......
1-tail, 0.05 level of significance tf b
c~
c~
q c~
I 2
S Fir. I qnI1OSO614damxlsx Bnturu by: l. Sumner Rev~iewed by.-N Page 50 of 117
TVA / Sequoyah Nuclear Plant, Intake Non-treated May 06-13, 2014
- ES Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date" 5/6/2014 Test ID: CdFRCR Sample ID:
5113/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnis dubia Comments:
Cone-%
1 2
3 4
5 8
7 8
9 10 Control-1 31.000 29.000 30.000 29.000 28.000 30.000 29.000 30.000 32.000 31.000 Control-2 28.000 29.000 30.000 30.000 29.000 33.000 32.000 30.000 30.000 30.000 10.8 32.000 35.000 32.000 32.000 32.000 29.000 30.000 31.000 33.000 30.000 21.6 33.000 30.000 33.000 33.000 31.000 32.000 31.000 29.000 33.000 33.000 43.2 36.000 32.000 35.000 32.000 34.000 33.000 33.000 33.000 35.000 36.000 86.4 33.000 32.000 38.000 32.000 36.000 34.000 34.000 37.000 37.000 35.000 100 37.000 38.000 36.000 35,000 33.000 36.000 34.000 36.000 35.000 38.000 Intake 33.000 31:000 30.000 31.000 33.000 30.000 32.000 30.000 33.000 31.000 Transform: Untransformed I-Tailed Conc-%
Mean N-Mean Mean Min Max CVDA N
t-Stat Critical MSD Control-I 29.900 0.9934 29.900 28.000 32.000 4.004 10 Contro!-2 30.100 1.0000 30.100 28.000 33.000 4.814 10 10.8 31.600 1.0498 31.600 29.000 35.000 5.420 10 21.6 31.800 1.0565 31.800 29.000 33.000 4.641 10 43.2 33.900 1.1262 33.900 32.000 36.000 4.495 10 86.4 34.800 1.1561 34.800 32.000 38.000 6.178 10 100 35.600 1.1827 35.600 33.000 38.000 4.016 10 Intake 31.400 1.0432 31.400 30.000 33.000 4.028 10
-2.137 1.734 1.055 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93101 0.868 0.58977 -0.3049 F-Test indicates equal variances (p = 0.69) 1.3125 6.54109 The control means are not significantly different (p = 0.74) 0.33646 2.10092 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.05479 0.03504 8.45 1.85 0.04656 1, 18 treatments vs Control-2 Dose-Response Plot dn 35 30 025 20 15 10 5
0 C8
1-tail, 0.05 level of significance (0
w C
c.~
~D (0
/
~File: sqn I0.050614dat.xlsx Entered byr J. Suver Page 51 of 117 Reviewed W t
gET Page 6 of 7 Date:
Species: Cerlodayhnia dubla Client: TVA/ Seguovah Nuclear Plant. Outfall 101 Da*y Chemistry:
1 Aaytidentified for each ft prormdKD.YO. fi', OrV41'5Y¶I teý
~
Ins ny I
~~0 a__
Analyst concentration IParaniter PH (S.U.)
DO (mP/L) conductivity (wnhos/cm)
CONTROL
'Mkaiinity (Mg CBC03IL)
(ng CaCOu/L)
- Temnerature M
[P Pu)
S -
O4 10.8%
Conductivity V 5 o "Icms/om) tC DH (S.U.)
7--
II -~--------~
-~~--=.-~--gI-'
DO (mgi'L) 21.6%
conductivity (Aimhos/cm)
4L
- Temoerature MC)
IPH (5.11.)
43.2%
IConductivity Z6 1(iunhos/cm)
- Temperature (00)15 nH (S.U.)
t s
DO (ma/L) 86.4%
I Conductivity (Wnmhwscm)
- Temperature (*)
pH (S.U.)
DO (maiL)
Conductivity q~mhos/cm)
-Alkalinity 10o%
- in. CaC,/
- ardness (Mg Chlorine (M 1
'*TRcebloine(,ng1L)
<0.10
_________*Temperature M")~
DH (S.qI)
.1.
DO (ma/L)
Conductivity (pmhos/cm) 10 0
100%
- Alkalinity I Intake (i g CaCO3&)
'H1ardne" 6
(Mg CaC03/L)
- TR chlorine (mg/L) 4
- Temperature (*C)
Lji.
Initial Final Initial Final Initial Final
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
Page 52 of.117 SOP AT1 I - Exhibit ATI 1.2, revision 07-01-12
Page 7 of 7 Species: Ceroikwhnla dubia Client, TVA /Segip'rahNusclear Plant, Outfall 101 Date:
O~-Ol.4'~
Dae ZU04!
I Day (Analst identified fbr each day. ne'fonned DH. D.O. and conductivitv measurements onlv.)
i S
3 I
4 I
5 I
Annlvn*
Concen-Parameter tiration I_________
PH (S.U.)
DO (mv/L)
Conductivity (Iamhos/km)
CONTROL
- Alkalinity (sag CeCOI)
MHardness Ca-A
- Temoerature MC) a -
'C PHSU3 DO (ma/L) 10.8%
Conductivity (umhos/cm)
- Temperature (IC)
U U
qp U ~
oH (S.U.)
21.6%
DO (mg/L) 9 lb -
U ~
Conductivity (gmho~om)
(PAO-iom) 4 i U.
v
- Temnerature C00 a -
dh a
pH (S.U.)
I I
lb-0 k S
-t.
AAM tl DO (mu/L) 43.2%
Conductivity fumhos/cm)
- Temperature (0C) oH (S.U.)
DO (m&/L) 86.4%
Conductivity (umhod/rm)
- Temnerature 00 pH (S.U.)
DO (mWL) 100%
Conductivity Wrahos/cm)
-Alkalufty (Pi asCOJIL)
- Hardness (Ing CaCOAI)
- TR chlorine (nrg/L)
- Temperature M) 100%
Intake
- Alkaihf,
- Hardness TRchlorine (ma IL bd.i.m b*wby SU.y I. b.
L 'ern eratureM I
'1 1360 1 'S0 11 LAo 11 L L" ZAg
- Temperatures performed at the time of test initiation, renewal or t-rmination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and tnscribed to this bench sheet by:
A SOP ATI I - Exhibit ATI 1.2, revision 07-01-12 Page 5 of 11
I TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 06-13, 2014
-Ceriodaphnia dubta Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0
__T Daily Chemical Analyses
-* Envlom ntal~sU1ng SoluUtons Imnc p
1 Concentration pairmeler I-I DH (51))
rm() (MVIL' IhJO I
a Initial Final Initial 8.03 8.00 7.99 7.8 7,9 7.7 309 300 58 6
87 6
Day I
Initial Final 7.93 7.98 7.86 8.0 7.8 8.1 302 62 F
t Da 3 Day 4 Initial Initial Final 7.90 7.93 7.95 7.92 7.7 7.9 7.6 8.0 302 306 60
- 62 941l 99 1
I Project number.
9773 Day5 Da6 Initial Final Initial Final 7.99 7.90 7.99 7.86 7.7 7.9 7.7 7.8 310 303 58 60 92 :4 t.uuuut.U Itt 1..J""I "'aY_
wtUI Control 09 UC!!11Lz & 1 4
lka-inity (mg/L CaC03)
Hardmews (mniL C&COW
_TemperatureC 24.9 251 249 24.9 24.8 24.9 24-9 25.1 24.8 U
- 24.
2.1 24.Y d.L PH (
8.04 800 801 7.92 7.88 786 7.95 7.93 7.97 7
99 7.91 8.00 7.86 DO (m/L)0 79
.0 7.8 81 7-9 78 7.8 878 7.9 7.9 7.9 10.8%
Conductivity (Ihose/nm) 305 295 M 313 3007 301 309P Temperature LC) 24.9 249 250 25.1 24.9 250 25.0 24.8 24.8 248 247 24.8 24.9 25.3 PH (SU) 8.05 797 81 7.92 806 785 7.95 7.92 7.98 7.94 8.00 7.91 8.01 7.85 DO (mg/L) 8.2
- 8.
79 8.0 7.8 81 79 7
7.8 80 7.9 7.9 7.9 7.9 21.6%
Conducivity (mhos/cm) 287 i
282 303 i278 289 2S2 293 Temperature CC) 24.9 24.9 25.0 24.9 24.9 25.0 25.0 24.8 24.8 24.8 24.7 24.8 24.9 25.0 PH (SO 8.05 7.98 8.02 7.92 8.07 7.85 7.94 7.94 7.98 7.94 7.96 7.90 8.01 7.82 DO (miL) 8.2 8.0 7.9 8.0 7.9 8.1 7.9 7.8 7.8 8.0 8.0 7.9 7.8 7.9 43.2%
Conduetivity (bmhos5m) 2 254 W2298 249 257
-250 256 Temperature (C)25.0 24.9 25.0 5.0 24.9 25.0 25.0 24.8 24.8 24.8 24.8 24.8 25.0 25.1 PH (SU) 8.05 7.98 8.02 7.91 8.07 7.85 7.95 7.94 7.97 7.94 7.97 7.90 8.00 77 DO 8.2 8.1 7.9 8.0 7.9 8.1 8.0 7.8 7.7 8.0 7.9 7.9 7.9 8.0 8&4%
Conductivity Oumhos/cm) 203ý 198 f
221 190 W I
195 18*2 190 1 Temperature (C) 25.0 24.8 25.0 25.0 24.9 24.8 25.0 24.9 24.8 24.9 24.8 24.8
-25.0 25.1 pHM 8.07 7.98 8.02 7.91 8.07 7.85 7.95 7.93 7.97 7.92 7.99 7.90 8.00 7791 DO (m0 )
8.2 8.1 7.9 7.9 7.9 8.1 8.0 7.7 7.7 7.9 8.0 7.81 7.9 8.0 Conductivity (anihos/en) 183
.4r&
M 176 02M V8 188 #RI
.167 174 N
ý 160-' LO!.
68~
100%
Alkalinity (migL CaCO3) 65
_S.
65 67 Hardness (ing/L CaCO3) 72 78101 64 Total Residual Chlorine (mg/L) 40.10 LM
<0.10c
<0.10 Temperature (C) 25.1 25.0 25.0 25.0 25.0 25.0 25.0 24.9 24.9 24.9 2
24.9 25.0 pH (SU) 8.10 7.99 8.04 7.93 8.05 7.87 7.95 8.09 8.01 7.93 8.01 8.
DO (M/L) 8.2 8.0 8.0 7.9 7.9 8.1 8.1 7.9 7.7 7.8 7.9
- 7.
7.9 Conductivity (sImbos/cm) 200{.
1 207 180 199 178 179 100% Intake Alkalinity (-/
CaCO3) 67IM M
"M.- ~.
65N 67 H1ardness (ing/L CaCO 3) 74 68 70
.1 Total Residual Chlorine (mal.)
<0.10
<a0.
<0.10 2.mperature 24.8 24 24.91 25.1
- 25.
24.9 24.8 25.0 25.0 25 9'
25.0 24.8 File: sqn101_0506i3chem.xlsx Entered by: J. Sumner Reviewed by:
Page 54 of 117
ETS Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seauoyah Nuclear Plant NPDES #:
Project #:
V1 County: a1 Outfail: 101 DI aon pr i on inrmation Comments:
Dilution prp (A%)
10.8 21.6 43.2 8614 100 Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL),
2230 1960 1420 340 0
Interferences.
ITOW volme (ML) 2500,
2500 2500 2500 2500 Test organism information:
Test Information:
Organism age:
'Al -
.0 Al "*AS Q kb Randomizing template:
.1, Date and times organisms
. O-b 0
M no Incubator number and were born between:
shelf location:
Organism source:
AThM.
6NT(.
of 4.*
.tq Artemia CHM number:
tqlE Drying information for weight determination:
Transfer bowl information:
pH I. IS S.U.
Date / Time in oven:
Os-cAh t'OA
,.Temperature =
-iA. t 0C Initial oven temperature:
1t0 C
Average transfer volume:
O P-Q Date / Time out of oven:_ _-tq-t%'
I Final oven temperature:
kA 1.
Total drying time:
Daily feeding and renewal information:
J Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Anal at Time Analyt Time Analyst Outfall 101 Intake 0-
-S0 6
1
-wm t
o An s
1
~
s o q
0 q
- A -
I_ o s -mn N c r b 4M CSI S
.1 - 4 5 2
O-O4.thI%
1c rLggo.4~
~'OScO. !A rN*S2AO OLD- 'g 3_
WwR-
.~-
Aa~t I.
AL o~L
~
4 6
os-IrL.14 U
N1411 At a.1%
Ai I -6s6q --Z I id~4sEI¶ZLL I-Cs4,qA 7I 1 1~~
VL~0 U
--I
.......... AT*-OYa*-c-d*fi*.-- --
-* *,, ;-m,*,7,TA,-*, ;e'*'* *,*"
- 31*-* I*-*-
Mortali:
- 01.
s20%
7-day LC50
ŽLQPL IAverage weight per initial larvae:
O. "101 NOEC I0O1.
Average weight per surviving larvae:
I 0.1o o0.25m/arvae LOEC
) l 01.
ChV
>)Cof.
EA1
__>8007.
Page 55 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
6 Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Date:
4S__.04184_
Survival and Growth Data Day CONTROL 10.8%
21.6%
A B
C D
E F
G H
I J
K L
0ll 10 A
/0 10 0 to 10 Q) 10 1
/0 1
1[o to 'o to I) 10
/0
,O'0./0 2
- 1) io to
/o
'0 IQ tO '0 0
1 10 "0
10 3
-to to 10 to. to 10 to 10 1 -(
t t
4 to 10* to, (0 IO) I0 I) to
- 1) 1( It to 10 5 _
_0
_0 11(
10 10 10 1O 10
/0 10 10 6_______
U~
()
Io ()
10 1
- 0) 10 10 Q0 to t 0
13 7
ID to 10 I0 to0 10 t0 to 10 10 10 A - P*o weight (mg)
Tray color code:: _
Analyst:
VA It4l41
'(A WT5. gnjN.Th jHO.
1~ 5.q5 p4.22z 1,.oR 1q. 14 1~44 Date:
-L1-'hb-tlIA B = Pan + Larae weight (mg)
Analyse.
VZ t
-3.4 (I t.(#,t ZS.14 2a.Ss 11.qZ 1.-4.21 2.".
Z*.j 31.S5 3.*-
Date:.
£X-fl.-lI C Larvae weight (mag) = B - A Handcolculated.
A
'14 '-'---
-1 w
Analyst:
Weight per initial number of larvae (mig)
= C / Initial number of larvae Hand calculated.
Analyst:
'.1
'9 0'
'.8 0
.01 07
'I 0
'V 1%
0 liN A.
Average weight per Percent reduction initial namber of from control (%)
- 0).10
-4larvae (i g)........;..........
m h
Sm 1mb m
I 0.fl2
-~.
ii.
a a
h Comment codes: c i clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
I~~ ~
~
~.
C.m.ts
.....1 II Page 56 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
PETS
'*----',....Page 3 of 6 Species: Pimephales Promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101, UV-treated Date:
,OS " -6 Survival and Growth Data Day 43.2%
86.4%
100%
M N
0 P
Q R
S T
U V
W X
II 00 0 /0
/0 t 0 ID /0 10 /0
/0 /0 /V 2
10 10
/i?
10 10 1U
/0
/t 10 10 r0 I) 3 tl0 I
C i
)
I )
tO 0 iIC L
I I C
[C) to 4
ID I0'cI io 1()0 1o 10 lo 1
/0 10 5
(0 10 ID 1(
.o 0 10 to 10 10 10
/0 /D 6
7_._
tO 10 lb 10 10
'0 IC
'0 1t 0
to 'C A = Pan weight (mg)
Tray color code:: I 05i" Analyst:
W l 51*0161 Date:
OL14.4.1 B = Pan + Larvae weight (mg)
Analyst:
'1i 2.'I.*0 24.Zq
%-% Zt.1(*, 'L
.S 23.3 22.I1
'i9.i 25.1) a,0.5 Date:
os-2 2-1 A
C = Larvae weight (mug) = B - A Hand calculated.
.101 O
.4t S 1' %
IS
- . g e).
Analyst:
Weight per Initial number of larvae (mg)
C C/Initial number of larvae A%
I Hand cakulated.
N Analyst:
z 0
- a Average weight per Percent reduction initilanumber of from control(%)
o"I,'
s 0,flS
-i,.7.
8 i
-jV.
-- e.* -...
Comment codes: c = clear, d = dead, fg = fimgus, k = killed, m = missing, sk sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded.
-O AT-0 --------
0 SOP AT20 - Exhibit AT20.3, revision 07-01-12 Page oI OT 771
5
~Page 4of 6 Species: Plmephales promelas Client: TVA / Sequovah Nuclear Plant. Ouffail 1011 uv-treated Date:
OS-O%-Iq Day 100% Intake
.Y Z
AA BB 0
/D
/
D /D/0 /0 C_ I ()I'0 /o/
2 oI /
o 4
0 6
_oW 10 0% 1 tj to A - Pan weight (ag)
Tray color code::
aImZ Analyt Date:
04A-2!-l B = Pan + Larvae weight (mg)
Analyst:
Ai Date:
M.4104 C = Larvae weight (mg) = B - A Hand calculated.
. 'eb 1.L Analyst:
Weight per initial number of larvae (mg)
-C l Initial number of larvae n
g Hand calculated.
C Analyst-Ab Average weight per Percent reduction initial number of from control (%)
a.TV4 1.
-larvame-r Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sA = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
-C om m ents." -......
WSV.I L V-.-n, Page 58 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
(9 TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 06-13, 2014 Pimephalesprhmelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control.
SVerification of Data Entry, Calculations, and Statistical Analyses
'5 Environmental Testina Solutions, Inc.
Project number.
9773
_Not fo Comr III e Aszefftr LaboratoryQC cm.ffn*rf
].tta Mist h16a1,am*.e r hflardblrem-A-P-.lglSt(.aj B-Pmn+Lz-r
£ lrmwdght(.a)
WdISonivling mekfgthdtl Cofieuiteh-ktIM WlMIhNIidull M..u..r,*,
M...lg4 allrMl cDsdd Pt, mfda-ee.
Wghl(.w
-A-2 wmlrorlar'e-(ulo Swvh 1amho.d a
.MP-t s
oF.a M (.g) 1%)
nio efb*.
Mfilb
.n.
ld I%)
I ML A
10 10 16.74 24.30
.7.56 0.756 0.756 Control B
10 10 14.91 21.41 6.50 0.650 0.701 7.6 0100.6
.701 7.6 Not applirabl C
10 10 16.80 23.41 6.61 0.661 0.661 D
10 10 16.23 23.60 7.37 0.737 0.737 E
10 10 14.78 23.14 8.36 0.836 0.836 10.8%
F 10 10 16.86 24.35 7.49 0.749 O.e2 8.0 0.749 100.0 0.802 8.0
-14.4 G
10 10 15.45 22.92 7.47 0.747 0.747 R
10 10 15.45 24.21 8.76 0.876 0.876 1
10 10 16.22 24.18 7.96 0.796 0.796 21.6% 1 10 10 16.09 23.33 7.24 0.7241 0.52 4.1 0.724 10O.0 0.7S2 4.1
-7.2 3
10 10 14.14 21.55 7.24 0.741 0.741 8L 10 10 16.44 23.90 7.46 0746 0.746 M
10 10 16.80 23.36 6.56 0.656 0.656 43.2%
N 10 10 16.52 24.301 7.78 0.778 0.763 11.4 0.778 100.0 0.163 11.4
-8.8 0
10 10 16.78 24.29 7.51 0.751 0.751 P
10 10 15.79 24.46 8.67 0.867 0.867 t o0
.10 14.64 21.26 6.62 0.662 0.662 86.4%
10 10 16.00 24.08 8.08 0.808 0.755 8.7 0.808 100.0 0.57 8.7
-7.6 S
10 10 16.05 23.58 7.53 0.753 0.753 T
10 10 15.78 23.73 7.95 0.795 0.795 U
10 10 16.34 24.19 7.85 0.785 0.785 100%
V I0 10 16.16 24.11 7.95 0.795 0..9 4.8 0.795 160.0 0.809 4.8
-15.3 W
10 10 15.82 23.70 7.88 0.788 0.788 X
10 10 15.37 24.03 8.66 0.866 0.866 Y
10 9
16.33 24.52 8.19 0.910 0.819 1 [a Z
10 10 16.14 23.54 7.40 0,740 0.797 10.8 0.740 97.5 0.774
&.5
-10.4 AA 10 10 14.72 22.87 8.15 0.815 0.815 BB I
10 10 15.89 23.10 j
7.21 0.72) 1 1
I.
0.721 1
O-WaI 161:
Duuumfo bMS value.
P5630:
Intake Deuneffts MELD
- Welu PNISID:
0.1016 14.5 0.0714 10.2 MED =
Minimum Significant Difference FMSD -
Peet Mirnum Significent Diffmauiee PMSD iso measur oftest precasion. The PMSI is the minimum perceu difference between the coutrol and tratment that i be declaned stwniically siofigc"mt in a whole eluent to Wi,-ty tenL Lower PMSD bouind determined by USEPA (10th pefrcctile) = 120.
Upper PMSD bound determined by USEPA (90111 pl*entile) = 30%.
Lower and upper PMSD hounds we determined from the 10th and 90th perccnlde, respecively, of PMSD dae from EPA's WET Imedabesaiy Vwrability Study (USEPA, 2001 a, USEPA, 2101b).
USEPA. 2001a. 2001b. Final teport: intzriabonatory Variabii4 Study ofEPA Short-term Chnmi and Acute Whole EUuemt Tar*city Tea Methods. Volumes I and 2-Appe"di EPA-821-B-01-004 and EPA-821-B-01 -005. US Enviromentel Protection Agency, Cincinnati, OH.
File: 8qn101 050614dats-uv tasx Page 59 of 117 Ere J.Smner Reviewed by; 3
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 06-13, 2014 Statistical Analyses
- EITS Larval Fish Growth and Survival Test-7 Day Growth Start Date:
51812014 Test ID: PpFRCR Sample ID:
TVA /SON, Outfall 101 End Date:
5113/2014 Lob ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA,821-R-02-013 Test Species:
PP-Pimephales Iromeles Comments:
Cono-%
1 2
3 4
UV-Gontrol 0.7660 0.6500 0.6810 0,7370 10.8 0.8360 0.7460 0.7470 0:8780 21.6 0.7960 0.7240 0.7410 0.7460 43.2 0.6560 0.7780 0.7510 0.8670 88.4 0.6620 0.8080 0.7530 0.7950 100 0.7850 0.7950 0.7880 0.8660 Intake 0.8190 0.7400 0.8150 0.7210 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean UV-Control 0.7010 1.0000 0.7010 0.6500 0.7580 7.503 4
0.7835 1.0000 10.8 0.8020 1.1441 0.8020 0.7470 0.8760 8.038 4
-2.395 2.410 0.1018 0.7635 1.0000 21.6 0.7518 1.0724 0.7518 0.7240 0.7960 4.119 4
-1.203 2.410 0.1016 0.7835 1.0000 43.2 0.7630 1.0884 0.7630 0.6560 0.8670 11.384 4
-1.470 2.410 0.1016 0.7635 1.0000 86.4 0.7545 1.0763 0.7545 0.6620 0.8080 8.745 4
-1.269 2.410 0.1016 0.7635 1.0000 100 0.8065 1.1534 0.8085 0.7850 0.8660 4.770 4
-2.549 2.410 0.1016 0.7635 1.0000 intake 0.7738 1.1035 0.7738 0.7210 0.8190 6.535 4
Auxiliary Testa Statistic Critical Skew Kurt Shapiro-VAlkas Test Indicates normal distribution (p > 0.01) 0.97956 0.684
-0.0952 -0.3987 Bartlett's Test indicates sequal variances (p = 0.63) 3.44692 15.0863
-l.othesls Test (11-tell 0.0 )
NOEC LOEC ChV TU MSDu MSDIp MSB MSE F-Prob df Dunnett's Test 100
>100 1
0.10163 0.14498 0.00611 0.00356 0.15174 5,18 Treatments vs UV-Control Linear Interpolation (200 Resamples)
Point 50 95% CL(Exp)
Skew ICos
>100 ICIO
>100 IC15
>100 1.0 IC20
>100 0.9-iC25
>100 0.8 IC4o
>100 0.7 IC50
>100 0.6 o 0.5 0.4 0.3 0.2 0.1 0.0
-0.1 -----...........
-0.2
-0.3 0
50 100 150 Doses e Dose-Response Plot D.8; 0.7~
0.8 0-4 0.53 0.2 0.1 1-tail. 0.06 level of significance Sgey L W.-f1 Page 60 of 117 c~
C'4 o
~
ce w
a File: sqrxl~lOSOSI4dsata-uv xlsx 2zltrerd byr. June Bavemwd by. A
TVA / Sequoyah Nuclear Plant, Intake UV-treated May 06-13, 2014 E frmvinmenta Teint eiang Sd Iam,l.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5&612014 Test IlQ PpFRCR Sample ID:
TVA/ SQN, Intake End Date:
5/13/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: May 2014 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Cono-%
1 2
3 4
UV-Control 0.7560 0.6500 0.6610 0.7370 10.8 0.8360 0.7490 0.7470 0.8760 21.6 0.7960 0.7240 0.7410 0.7460 43.2 0.6560 0.7780 0.7510 0.8670 86.4 0.6620 0.8080 0.7530 0.7950 100 0.7850 0.7950. 0.7880 0.8660 Intake 0.8190 0.7400 0.8150 0.7210 Transform: Untransformed 1-Tailed Conc-Ph Mean N-Mean Mean Min Max CVA N
t-Stat Critical MSD UV-Control 0.7010 1.0000 0.7010 0.6500 0.7560 7.603 4
10.8 0.8020 1.1441 0.8020 0.7470 0.6760 8.038 4
21.6 0.7518 1.0724 0.7518 0.7240 0.7960 4.119 4
43.2 0.7630 1.0884 0.7630 0.6560 0.8670 11.384 4
86.4 0.7545 1.0763 0.7545 0.6620 0.8080 8.745 4
100 0.8085 1.1534 0.8085 0.7850 0.8660 4.770 4
Intake 0.7738 1.1038 0.7738 0.7210 0.8190 6.535 4
-1.980 1.943 0.0714 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p 0 0.01) 0.79891 0.749
-0.0103 -2.5753 F-Test indicates equal variances (p = 0.93) 1.11097 47.4683 Hypothesis Test (1-taill 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.07138 0.10183 0.01059 0.0027 0.09497 1, 6 Treatments vs UV-Control Dose-Response Plot I
0.9.
0.8 0.7-0.6 90.5, 0.3!
0.2 0.1 1-tail, 0.05 level of significance Cl N
o tC File: sqn101_050614data-uv.xlsx Entered by: J. Sumner Reviewed by-Page 61 of 117
a5ETS Page 5 of 6 Species: PimehaLes to-melas Client: TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Daily Chemisty:
Date:
-O, LI I
Day (Analyst identified for each da performed pH D.O. and conductivit measurements only.)
0 Awlysl I
1 I
2 I
Concentration jParameter oH (SU.
-7_
H --,.
=
DO (mtrLI Conductivity (uimhos/cm)
CONTROL UV-treated
- Alkalinity (MR CaC0a/L)
(mg aCOAL)
- Temuerature (*C1 I
6 ~tI~
pH (S.U.)
DO (mt/LU 10.8%
Conductivity (iimhosicnil
- Temperature (QC)
PH EH(U.)
I~
f IDO (mg/L) i4 21.6%
IConductivity
- MB'o U
(jzmhos/cin)
I *Temnperature (*C)
IS
~Tt I
-~ A "UL.q I
2-9 nH 'S.U.3 e'y- > 1laA)td
.51 pH (&U)
DO (ma/L)
BA0 43.2%
Conductivity 2-41 (Pmhowm) 6 (
- Temperature (QC)
U-%
14,1 U ~U DR (S.U3 R.AY1 I
ii DO (*m/LI 86.4%
Conductivity zo
- Temnerature M I
vH (S.U.)
9 ~
DO fromt/
100%
(Mg CELCO31L)
- EHardns1
- TR chlorine (wnWL)
- 10
-M s
CK0%
~0.1 r
I-J
- TPmnernhatirm t-1 --.-
oH (S.UI Conductivity (pMhos/cm)
Z
_*Alkalinhty....
- Hardness
- TR chlorine (mg/L)
- Temoerature (00 M.1 U,
a a
~
Initial Final I
In nA nitFina
,Jratmures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
(. Hd-Y kral, t, Ehardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
Page 62 of 117 SOP AT20 - Exhibit AT20.3, revision 07-01-12
IgET$
- '---"---'*Page 6 of 6 Species: Pimehales gromelas Client: TVA/ISeuuovah Nuclear Plant. Outfall 101. UV-treated Date:
c*.t.J Clet:TA euoahNcla Pat.Otfl 11 U-retd ae:
0-0-L I
Day (Analyst identified for each day. performed pH. D.O. and conductivity measurements only.)
I M
I I
Analyst I 1
I 4
I Concen-Parameter tration I
PH CS.U.)
DO111191)
CONTROL UV-treated Conductivity ld=!ý i
- AMBallnty frog CHCOq/U) a
- Hardness (Ing CaCO1/IU 0 ~2
- Temperature (*C)
IPH (S.U.)
-K IDO (nggL
.i 10.8%
Conductivity
'2 gimhos/cm)
________*Temperature
(*C)
ISO j0 PH (S.U.)
I '.1*
DO (mgLM 21.6%
Conductivity (umhos/cm)
- Temperature (*C)
DH (S.U.1 DO (mW/L) 43.2%
Conductivity (wmhos/om)
- Temperature (C) 0H (S.U.)
DO (01/L) 86.4%
Conductivity (1mnhos/(WCm
- Temoerature (0C)
PH (S.U.)
DO (me/L) 100%
Conductivity (tjmhos/cm)
- Alalinity (Mug COCOAI)
- Hardness (mg CaCO3/L)
- TR chlorine (ngL)
-*Temperatur(C0C..... I ---
Conductivity
- Hardness
-*TR chlorine (znajL)
- Temperature Q
- *~
"L L
I -e,
-M*
InitIal Final Initial Final Initial Final Initial Final i
t ia a,
I Final I
Initmiraln1 eratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal information table located on Page 1.
VAB kL *VAMniy, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet Total residual chlorine was performed on non-treated Outfal1 101 and Intake samples.
M SOP AT20 - Exhibit AT2203, revision 07-01-12 rage DJ OT 7 71
Ai l
ETvr esnt il~
ng Solution;, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated May 06-13, 2014 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number:
9773 Concentration Parameter DA I
I Da 2 Da Initial Initial Fi I
Idw Ilnitial Initi Final Initial Final PH (SU) 8.04 8.03 7.70 8.06 7.65 7
67 7.97 66 7.98 7.61 8.02 7.67 DO (mnwL) 8.0 7.9 7.3 8.0 73 7.9 77.9 7.1 8.0 7.3 7.91 7.0 Conductivity (nMos/em) 321 31 31 3133 31 314 Control Akalinity (mg*L CaCO3) 61 61 62 6
63 Rarduess (nWJL CaCtI3) 9290 i
8 96>
s0o 88<
Temperature (CL 24.9 24.6 24 24.7 24.9
- 24.
9 24.6 24.9 24.6 24.8 24.6 24.9 24.6 P8 (SU 8.05 7.84 7.97 8.06 7.94 7.67 7.94 766 7.9 7.63 7.96 7.71 10.8%
DO (me!LU 8.0 7.9 7.9 7.9 7.7 7.7 7.9 7.0 7.6 73 7.8 7.1 Condnctivity (
smho/em) 299 311 3'4 1320 299 297 298 '.*
T Temperature (0C 250 24.7 24.9 24.6 249 24.6 25.0 24.5 25.0 24.6 24.8 24.7 24.9 24.5 PH (SU) 8.05 7.84 7.99 7.60 8.06 7.61 7.95 7.63 795 7.64 8.00 759 7.98 7.71 21.6% DO (MAIL) 8.0 7.9 7.9 73 8.0 7.4 76
- 7.
7.9 7.0 76 73 7.8 7.1 Conductivity (umbos/em) 286 294 =>.
306 301 222 283 Temperature CC) 25.1 24.7 24.9 24.4 25.0 244 25.0 24.5 25.0 24.7 24.8 24.7 25.0 24.5 pH_ (SU) 8.06 7.84 8.00 7.67 8.06 7.57 7.93 7.67 796 764 8.00 757 7.98 7.70 DO (_
_L) 8.1
.8 7.8 7.3 8.0 7.4 7.6 7.6 80 70 7.6 7.2 7.8 7.1 43.2%
Conductivity 0mhos/em) 26!
266 297
-259 250 240 254 Temperature CC) 25.1 24.7 24.9 24.7 25.1 24.5 25.0 24.6 25.1 247 24.9 24.4 25.0 24.3 PH (su) 8.07 7.85 7.99 7.66 8.07 7.56 7.94 7.67 796 760V 8.00 7.56 7.99 7.65 86_4__DO
_m 8.1 7.8 7.9 7.3 8.0 7.4 7.6 7.6 8.0 69 7.6 7.2 7.8 7.2 86.4%
Conductivity (pmbos/cm) 208 209 216 21 19-
. 192______
Temperature *C) 25.1 24.4 24.9 24.5 25.1 24.5 25.0 24.2 251 247 24.9 24.7 25.1 24.5 pH (SU) 8.08 7.89 8.01 7.66 8.06 7.53 7.94 7.67 7.96 7.61 8.00 7.54 7.98 7.65 DO (mWI.,)
8.2 7.8 7.9 7.3 8.1 TA 7.6 7.6 8.0 6.8 7.6 7.1 7.8 7.2 Conductivity (junhos/cm) 189 7
186 190 175 166 164 169 100%
Akalinity (mg/L CaCO3) 65 63 61 Hardness (mg/L CaCOW) 70 r
66 i
64 t Total Residual Chlorine (ma/L)
<0.10
<0.10
<0.10...
Temperature 0C) 25.2 24.6 25.0 24.5 25.1 24.4 25.1 24.2 25.3 225.0 24.5 25.2 24.7 pH (SU) 8.11 7.8 8.01 7.68 8.06 7.61 7.95
.7.77 7.97 7.18.0 7.65 7.99 7.71 DO (rag/L) 8.2 7.9 7.9 3
8.2 73 71 7.6 8.1 7.7 7.0 7.8 7.2 Conductivity (itmhos/em) 204 191 20 191 1
182 183 100% Intake Alkalinity (mg/L CaCOt) 67 65 67 0_.-
f Hardness (ma QLqCO) 68 74 70 T otal Residual Chlorine (mnJL)
<0.10
.=-1 r
<0.1<0.0.10...0.
-Temperature (QC)
- 25.
2 25.5 224.9 24 24
- 25.
24.
- Note: Total residual chlorine was perfbomed on non-treated Outfall 101 and Intake samples.
Page 64 of 117 File: sqn1O1.OOO614chem-UV.xlAs Entered by: J Suiner Reviewed by:
0 DETS D ft analyed ao!,?-ý.Jq I
Page 0 Page
-Lof.
Time initiated Time completed [ 0819Z Alkalinity (SM 2320 B-1997)
Matrx Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
pH of Normality NoIty (N) of H2S0 4 pH Factor or Multiplier Deiontzed Titrant check Begin End Total (5 ml Na1COs x.0.05)E
= (N x 50000)/ 100 ml sample water reference standard ml ml ml
= 0.25/E
= Nx00
= 4.5 S.U.
number number (E)
(aceptable ruge 0.0180 -0.0220)
I ~
Ik tW 100 100 2,_____________________
G6 V -
I I
Re*erence standard True ve Sample Alkalinity (MV)
% IRS = MV / TV 100 number volum Bein End Total Multiplier (gCaC
)
(acceptablerag
- ,(Ing CaCO1)
(10i) 011 al EMI 0t 1%
t~~t*
zoo
.100 12.:5 ZA~
!2-
- q DaUp/cate Ran, v/predslon,"
sample At~lyr Sample Sample ED volume Begin End Tlt9S Multlier (Mg CaO3/)
((8 -D)/4(+D)/21) x 100 number (nil),. ml
. ml ml (aeptablerange=-_-.1_%)
.Ma&,& ' s)p:l7See (A)
Rederence standiard Spike value Sample Spike alkalinity (A) number (SIV) volume Begin End Total Multipler (m* CaCG3/L)
(mg 9DC-_,
1 l
)
ml ml ml r4x Ust*,
0"N5 /00 N-T A2. 1
- 10. 2-Cq Sample alkalinity (R)
Measured spike Yak* (MV)
%R=MV/SVX100 (rag CaCOaL)
MV k A -B (Acceptable range (g
QS
-gf~,
-75 to 125%)
.*1__
Sample volume Begin End Total Alkalinity Sample number Sample ID (m!l ml ml
.1 Multiplier (mo CaCOsAL) 04-284%1 C0-0*
Jim:
92.i. K, 1 3,0 1b.z 31 Vi -76.~
114A 30110.49 4.*,iA fl' Hi.,
p.q se.8 OH. -ro5.-H4 GU 123A*I I-Reviewed by-iIZI-Date reviewed:
o4"'M.
SOP C6 -Exhibit 06.1. revision 01-01-13 Page 65 of 117
S F
dk~
-K
.~t.
~
S.ETS Page 7_
Page_
of_ ___
Time initiated [
q j Time completed LZ-Z, a
Analyst L~77 Date analyzed d~iiII Thwnxt normalit and mualgiciep detemakaatipy Alkalinity (SM 2320 B-1997)
Matfri: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
_.oNormality]
Normality (iV of Hz$O4, pH Factor or Multiplier
- Deloalzed Titrant cl--e-e-e-e-e-e-e-Za&_ Total
=(5 ml Na2CO3 x 0.05S)/E ff (N*x 5000)/100 ml sample water reference standard ml m
-l
= Nx 500 fi4.5S8.U.
num ber number (E) trtaern*
Labraora control stan da__..
Rderence standard True value Sample Alkalinity (MV)
%RS=-MVITV/*i0 number oTV) volume Begin End Tota Multiplier (mg CCO3/L)
(acceptable range (Ing CaCOs) L l) ml Jl mi
- 90 to 110%)
I00m5 100 100
£54q U.19
)-
A D,plicates le pOMtc I
Sample Alkalinity ORPD Sample Sample ID volume Begin End Total Multiplier (mag CCO3/L)
((S - D) /[(S+D)2j]) x 100 nmead alml (aceeptable range -* 10%)
numbert (13 7ml) 512 D
ml...
Matrix sike recover:
Rdoence standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOa/L)
(129 CaLCO;)-
(101) ml ml ml Sample alklinity ()
Measured spike valueI(
%R=MV/SVx1OD (Ing CIC/L)
MV = A -D (acceptable range (mjI cRcos/)
=75 to 125%)
Tb0 CIO.07.
-Sample volume Begin End Total Alkalnity Sample number SampleowD (mi) ml ml ml MuCper (m
aCOI )
11,5300 b'~i A-MO
_ I3 4 3L 10 Reviewed by:.
Ii~I Date reviewed.
SOP C6 -Exhibit C6.l. revision 01-01-13 Page 66 of 117
lETS Page
'U Page of Analyst I
i Duew*ed M 10
]
Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time itiated S-Time completed 140 1r pH of Normality Normality (N) of HES0 4 pH Factor or Multiplier Delonuzed Titrat check Begin End Total
= (5 ml Na2CO3 x 0.0)1/
f (Nx 50000)/100 ml sample water reference standard ml ml mi
= 0.25*1
= N x
=4.5 S.U.
number
- n. umber (E)
(aceeptable range = 0.0180 - 0.0220) 6.0W)6 Iit~
[
4NAil 0.2-NO1 0.0 ?O~ z. -
10.1 Reece standard True value Sample Alkalinity (MV)
% RS = MV /TV x lee number (TV) volume Begin End Total Multiplier (ng CaCO0/L)
(acceptable range (mg CaCOs/L)
(ml) ml ml ml
=90 t 11o%)
~~100
,00 17z..
aCp o!
q qq47.
DUPnak m pk precison:...
SAlklinity
%RPD=
Sample Sample ED volume Begin End Total Muli plier (wgc cBCO.3 L)
{(S -D) I[(S+D)/2]) x 100 number (ml)
.ml m
il 0 (acceptable range
-10%)
SSW10 22.1 1ALL b
L Dupf cMS)
.1fp
- S AIZ a
-1D Matrix spike recove:..
ReIrence standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (ng Ca&C)s/L)
(Ma CaCOIL)
(1100 Mnl.
ml ml (219 CaCOdL)
MV=A -B (acceptable range (Mg CaCO*.)
75 to 125%)
Sampl measuemeaL:
Sample volume Begin End Total Alkalinity Sample number Sample ED ml ml ml Mutiplier (Mig CRUC0/L)
- ,i~a IU..
- ..3L2
$,1.
104 32 OS.
31,#.4
__2_1 t6.oeaa-4 A Ch. I AD S.0 1 O6Oc.N
.**.1*4 If.
Os5oi. i'
-I
-4I 1_ 10. 1S
-Ito__
Reviewed by:.Z Date reviewed: I J
-1 I
SOP C6 -Exh*bit C6.1. revision 01-01-13 Page 67 of 117
SETS Iflv*MmflmernIV biin oUl'lin*Inc, Page I2-of -
Alkalinity (SM 2320 B-1997)
Mairix: Water, RL = 1.0 mg CaCO3,/L Analyst 6*1 Date analyzed CC.~
Titrate samples to pH = 4.5 S.U.
Time initiated [
7 Time completed at normal and mwUfen-pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml NaCO, x 0.05)/E (N x 50000)/100 ml sample water reference standard ml ml ml
=0.25/E
- =NxS0 4.5 S.U.
number number
_E)
I (acceptable range = 0.0180 - 0.0220)
I
-I c6.1__
1I Mafrix stpie recov erj:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mag CaC0+A)
(mgCaCOs/L)
(nd) ml ml ml Sample alkalinity (B)
Measured spike value (MV)
%R=XV/SVx10 (ag CaCO3L)
MV=A-B (aeeptable range
(.g
,CaCO-3 IL 7 to.15%)
awe, measurema:
,,ts-Sample volume Begin E!d Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mR CRCO/L) o4.*.jC.-
f tM W
- Inc, 9A 6.o
______A I
4- &,
(0_
ISO -Z2.g 1 4.
-o
%.lc. A i+q L4...
._ o o-"
4_
f oL
- .ed~~t,4fiX *
=&uy,- r,.trg Oa-lo'14.6 5'ýo.
qo.:
OA Reviewed by:
I Date reviewed:
SOP C6 -Exhibit C6.1. revision 01-01-13 Page 68 of 117
+
~,~4.
I-2*
C I
I I
I I
0 j~~utIe~gEu~a.ht Date analyzed~
7*rawi,~nnM and
.4~~,
aa in Mkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCQ3/L Titrate samples to pH = 4.5 S.U.
rago _L..
Page 3
of_
Time initiated Time completed u-pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total t (5 ml Na2CO3 x 0.05A/E
- (Nx 50000)/100 ml sample water reference standard ml ml ml
= 0.15/E Nx 500 4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Labratory cntrol standard:
Reference standard True value Sample Alkalinity (MV)
%RS=MV'TVx 100 number (TV) volume Begin End Total Multilplier (Ing CRCOS/L)
(acceptable range (Ing CaCO3,)
_)
ml ml
_l 90 to 110%__ )
1~g It2*
100 100
.O.
2 IO.t 9')
3 tcate sam pleSprecmlop:
Sample SampleI) volme Alkalinity
%RPD Sample SBegin End Total Multiplier (g CaCO3/L)
((S - D) /(S+D)/2I) X 100 number DIDml___
(acceptable range =- 10%)
Maeire aL_
alaiitA Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mrg CaCO3L)
I(rag CRaO$)
(mI) ml ml.
ml.
Sample alklnty (B)
Measured spike vle(V R
M/SVz 100 (Ing CaCOAL)
MV = A - B (acceptable range (aCRC%&I) 7 25 toW) 24O~
Sawmwe measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mng CaCOsb)
I-%
.tw -
S7
,LL 0.0 f 1L(.00.1 2 2.
0 VA erS..
labi V. A~ 2-14 j
~~
31, J
2-g AL
.01 jsjjut 111 2~34 L.o 2-51415 2+/-
?IA~
Reviewed by:
,I
-1 Date reviewed: I 0 S-I SOP 06 - Exhibit C6.1. revision 01-01-13 Page 69 of 117
I -'!
.I..
I.t.
eE bTnS~hlm Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO/£L Page of Time initiated Time completed Date analyzed 061j jjj Titrate samples to pH = 4.5 S.U.
LaboratoryJ conirof stwdard.
Referenic standard True value Sample Alkalinity (MV)
% RS = h1 / TV x 100 number (TV) volume Begin E
End Total Multiplier (tag CaCO/L.)
(acceptable range (nag CCO
)
(ml )
ml ml al
- 9 to 110%)
AplIItt 100 100 0,0 q.S q..
- DiU, s
-w I
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOA,)
((S -D) /I(S-D)a2} x 100.
number (ml) ml ml ml (acceptable~range =: 10%)
D
_/_
Ma&r spike recoeiy:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Iag CeCO/)
(mR CaCOA)
M) ml ml ml Sample alkalinity (B)
Measured spike value (MV)
%R-MV/SVxI00 (tag CaCO3/L)
MV= A-B (acceptable range (mg CaCOL)
- 75 to 125%)
Sample measuarenentg:_________
Sample volume Begin End Total Alkalinity Sample number Sample EID (ml) ml ml ml Multiplier (MR CaCOs)
Pt6Ot--.C
-Cg9 At 7,(
1-"!
z*.
,o
.,3 341 V.
S!.-"
- 3.1
.M-1i.>
65Q 0W J-O-.3 1T Reviewed by:
I At. - I Date reviewed: I a p$.*
I SOP C6 - Exhidbit C6.1. revision 01-01-13 Page 70 of 117
"ETS Date analyzed
-/
dI Tftramt norimalits' And iuWdilRg detewdnnhaad rlage Page S
of Alkalnity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time initiatedI Time completed pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na2CO, X 0.05YE
= (N x 50000)/100 mi sample water reference standard ml ml ml w 0.(2/E
= Ni 500 4.5 S.U.
number number E)
(acceptable range =0.0180 -0.8220)
At 06.s-lo-Mabfr znike
- imeri, Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begn End Total Multiplier (ag CaCOBIL)
(UK CaCO.)
NmO Ma l
ml Ifel' 26=
30 1,
Sample alkalinity (B)
Measured spike value (MV)
%R=MV/SVx100 (Cg CO,/L)
MV-A-B (acceptable range (mg CaCOCNL)
= 75 to 125%)
'9.0'?
Sample measmurmets:
Sample volume Bein End Total Alkalinity Sample number Sample ID (0l) ml ml ml Multiplier (MR CaC0DiL)
- Dow, (i
- Oli-
.4 I
ý,
14~l.
a*
.1 I.J.A 2,H.S 5.0 IqI tosjoo,-
I 2-00 i
.4 i
Reviewed byI Date reviewed: I OS'o- 'j SOP C6 - Exhibit C6.1. revision 01-01-13 Page 71 of 117
Page 1
of L.4 Aikalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Aualyst L AA~ZI Date wanlzed ()r>11J1 Time initiated [
7 Time completed LI!i2 i
Titrate samples to pH = 4.5 S.U.
71irwd normaiftv and madbioler determinatiorn pH of Normality Normality (A) of H2S0 4 pH Factor or Multiplier Deionized Titrunt check Begin End Total
= (5 mI Na2CO3 x 0.05)/E (N x 50000)1100 ml sample water reference standard ml mad ml
- 0.25/E
=Nx500
=4.5 S.U.
number.
number (E)
(acceptable range - 0.0180.0.0220) 6A JOS5I6k?4I0 2 (
1&0 rO.
. Oz i&.i Reference standard True value Sample Alkalinity (MV)
%RS=MV/TV*100 number (TV) volume Begin End Total Multiplier (Ing CACO3L)
(acceptable range (Ing C&COA/)
(1111) mld ml ml
= 90 to 110%)
100 100 1
2.io cis cis. 0,70 Dupliae*s le precdsion:
Sample Alkalinity
-RPD Sample Sample ID volume Begin End Total Multiplier (ag CaCO3/L)
{(S - D) 1t(8+D)12)} x 100 number (m_)
ml ml.
ml (a2ceptable rane=" 10%)
nS-V).jq65 1",ui too z7.z.
to,%
Matrix spike recover,:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Iag CACO3/L)
(Mg CaCOSIL)
(mI)
Jul m
ml
- Iso
%r~i JD.-
jc(o 10.1 kin Sample allWinity (B)
Measured spike value (MV)
%R=MV/SVx 100 (Ing CaCO3/L)
MV = A - B (acceptable range (n,
g C( C k"
75 to =M%)
Sample volume Begin End TOtl M
Alkalinity Sample number Sample IOD (Inr ml m1 ml Multiplier (MR CaMIL) 054Is-114
[_ LJ.9 o~e
.,. -,I-V&.
s L0A b SWU VAa SHE C) 9A
- 50.
U 2-
_____CA O____
1.6A 2Z.2-
_+_
20 LS'2$
_.__.U 3,-oS.
1I 3..
_A?_._2-_
1?-1 3o 3.2-
.r i
'AG1D bIi~
Reviewed by:
I
,I Date reviewed: 1os-lk.P4 1
SOP C6 -Exhibit C6.1. revision 01-01-13 Page 72 of 117
ITS Analys;t[7 nat analyzed MI diik4 Page Page. _2ofj Alklmnity (SM 2320 B-1997)
Matix: Water, RL= 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time initiated M~me completed[IIjI 1
1r pH of Normality Normality (0) of H280 4 pH Factor or Multiplier Deionized Titrant check Begin End Total f (5 Ml Na 2CO3 x 0.05A)/E (N x 5000)/ 100 ml sample water reference standard ml ml mi
- 0.25./E
= N xS0 f4.5 S.U.
number number
)
(acceptable range -0.0180 - 0.0220) mi Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% S=MY/IV X 100 number (TV) volume Begin End Total Multiplier (mg CaCOVL)
(acceptable range (mg Ca*]dL)
(ml)
Inl ml mll
- 90 to 1109A)
I~i7.q 100 o100 *.S q66 q-1 tu,.1 S2_
9z, In%
Sample Alkaliiy"%
D Sample Sample ID volume Begin End Total Multiplier (mg CaCOA.)
{(S mID) /[(S+D)2J) x 100 number CO(Bl) ml ml ml (acc eptable range
. 10%)
Mah* spi
,,ke -
Rehrence standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Tot l
Multiplier (Ig CaCOaL)
(m co )
(ml) ml ml mal too 3.2.
t, L
'A.1-Sa*mple alkalinity (18)
Measured spike vale (MV)
% R= MV /SVIx 10 (ing CaCOL)
MV-iA-B (acceptable range (m_ _ C__ _ _
75 t-W % )
Sample
__eat:
Sample volume Begin End Total Alkalinity Sample number
.Sample IUD (01l) ml ml ml Multiplier
"(a ad) 1 % ¢aA.'.
hs
, %- A o),
go, B D
-o I0,1 3.-o d)
ID.A I %k 4
.OA
- .L I
- -32.0L 23.T*
3,_.&*
(o5
,v.osogs' # OC A2 SMU
- 23s, ZVI.Z 1-k"
,,.n-Reviewed by.
[
1Z Date reviewed: L' 6&.iii SOP C6 - Exhibit C6.1. reviion 01-01-13 Page 73 of 117
S.ETS
- E..r~ ~ ~~~flueml'w g S luofl.
Page
-1 Page __
of Analyst Date analyzed Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time intiated [ -
7 Time completed [
-I 1
-IL-*
Jr nl IýItt,,.m l~
md~/
Reference standard True value Sample Alkalinity(V)
%RS=MV/TV x110D number (TV) volume Begin End Total Multiplier (ms CaCOj*L)
(acceptable range (019 CaCO3/L)
LMi)
'10 M
I 101m
= 90 to 110%)
lINCz2_V 100 100 o
_ q,'
q t3 10-1 9
,3 Dx/&Ale sa ple Pr on:_
Sample Alkalinity
%RPD=.
Sample Sample ID volume Begin End Total Multiplier (mg CaCOL)
((8 - D) /[(S+D)/2] x 189 number
__(mI) mi mi.
mi I
I (acceptable rnge =e10%)
_Duplicate(B)
- 1.,
D Matrix spike recoverM :
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CoCOs/L)
(Os/CaCO3L)
(MID mi ml mi Sample alkalinity (B)
Measured spike value (MV)
% R='MV/SVx1O0 (rag CaC)D/L)
MV A-B (acceptable range
, (amg CaCOjL)
= 75 to 125%) )
SaMmnle meassmreinenftw:_____
Sample volume Begin End Total Alkawiity Sample number Sample ID (MI) ml ml ml Multlier (mg CoCOs/L)
TVoAo I q.b 46O
_I
_Za, L.5 q'_.
1
...3 37,0 Ck I
~ i2-o Lr, m
- 4oIo~I.3 0_
__00 4-5 C1 9_
146SO
-'O 7VLACL& I VIWu I
-I z-9
%%4.Q S -0a I I__
I.~
?w$_LA.,-__I t,4 avo t
Reviewed b3 Date r
- viewe I'
SOP C6 - Exhibit C6.1. revision 01-01-13 Page 74 of 117
Page -T7 Page c-*Lof -jH AnalystLI7 777 Date analye7A iiII Modan nonnaliAv and,melipier determinadaioi Aikajity (SM 2320 B-1997)
Matrbc Water RL = 1.0 mg CaCOA/L Tiftate samples to pH = 4.5 S.U.
Time initiated Time completed [I
-i /u:*
-f pH of Normality Normality (N) of H2 SO4 pH Factor or Multiplier Deionized Thtrat check Begin End Total
= (5.l Na2CO3 x 0.05YE
= (N 50000)/100 ml sample water reference standard ml ml ml
= 0.25/E
= Nx500 4.5 S.U.
number number
(-)
(acceptable range
.0180 -0.0220)
Laboratory controIsandarL_
Reernce standard True value Sample Alkalinity (MV)
.%RS =MVI/TV XI 10 number (TV) -
volume Begin End Total Multiplier (nag CaCO/L)
(acceptable range (rag CCOs/L)
(01}
ml4 nAll
-90 to 110%/0)
INS if?--4 100
'00.l' 7.,:
t' Ot 9fO Di cates c pzreclio:.
Sample AlkaliSity aRPpl Sample Sample ED volume Beein End Total Multiplier (tag CaC(WL)
((S - D),*[(8+D)t2]) x 100 number (ml) ml ml ml (acceptable range IOOA)
Matft spike recov :
Referenee standard Spike value Sample
.Spie alkalinity (A) number (SV) volume Begin End Total Multiplier (aCg *OsIL)
I (UM-LCmal)
(101) ml mal ml Sample alkalinity (B)
Measured spike value (MV)
%R=MV/SVIOO (nag CaCOsL)
MV = A - B (acceptable range (10, CACOSIL) I7 to W%)
Sample meavur ts:
Sample volume Begin End Total Alkalinity Sample number Sample E[)
(0l) ml ml ml
.multiplier (In CaCOA/LI
_OS__,_.
9i.
w iCS.O~t eL- (a2,Q qL j qj jaoso ai.o IVA OtAF L47A I__'
a1.L.
2.__
tO_.___t ae
.__',,*lt,
- I oi a-Reviewed by:
t Z
Date reviewed:
o
".1i.-.i SOP C6 - Exhibit C6.1. revision 01-01-13 Page 75 of 117
I I
p I
I S
0 p
0.ETS
' bwbsum~T.glng SdudIum.k~
.rage VMa' Pa*e _J of I
Time initiated [I 'it70 7i Time completed [
ll Analyst V-IZIII Date analyzed I R:
Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3IL reference standard ml ml ml
=0.=/E (Nx 50000YSo5 ml sample number number
_)
(acceptable range - 0.0180 -0.A020)
= Nx 1000 lwtsI At)Aiy oo 0.0 Oe2..--
Reference standard True value Sample Hardness (MV)
% RS= MY /TVxlo10 number (TM
. Volume Beg~in End Total Multiplier (mg CaCOVL)
(acceptable range (rag C*C0H)
(1d1)
U1V ml m%
to 110/T0)
I40 50
-0 2.0 20.2-D z~aape prechion:
Sample Hardness
%RPD f Sample Sample ED volume Begin End Total Multiplier (mg CaCO,/L)
((8-D) /[(S+D)12} x 100 number (m]).
ml all mlII b-4-e-ILL4 1 Q 1_4. 0 2.
Z,.02-s (4Z D
uplicate(B)
IJ iq o 2,1
.4 D
2-tO
' z Mai*~
WIke Reference standard Spike value Sample Spike hardness (A) number
.(SV) volume Begin End Total Multiplier (mg CnODX/L)
.(mag CaC0)
(ml) ml ml ml
,4.0
- 9) 1-g.
D 1J9-Ie_4_.1 ZO._
Sample hardness (B)
Measured spike value (MV)
% R = MV SV x 100 (mg CoCOs/L)
MV = A-B (acceptable rauge (mg Cco/)
=75 to 125%)
I Li_
'I i2, Sample measreia__m_--
Sample volume Begin End Total Hardness Sample number Sample iD ml ml l
Mutiplier (m
COL)
TV =ND:
Blank (should be -0 mg QaCO3/L) b 0 o
.. O 20.2
__._10_,.6.
SSWO a It Z-o I:2 D.,
o1441 26 4 b:
20 1 q,0 o/Q Note.: if >15ml of trat is used, smple must be diluted.
Reviewed by:. L IZ Datereviewed 4j.
SOP C7 - Exhibit C7.1. revision 01-01-13 Page 76 of 117
0ET,I0101b Page f
ofL3k Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCOL Analyst F t 7
Date analyzed [Ie,,%.lZ]
TrIuiW iormalfu amai nkuirdtrbaln Time initiated 1
- t Time completed
( *54 Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reerence standard ml ml ml.
= 0ASE
= (NIx S0000)50 ml sample numer number (E)
(acceptable range - 0.0180 - 0.0220)
.N.
1000 L
ILWcontroL stanard Rerence standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number volume Begin End Total Multiplier (mg CasC0/L)
(acceptable range (nrg CaCOTV
)
(ol) ml B
il ml 90 to 110%)
Dwkiicate sap ie prrelon:
Sample HardnessmRPD Sample Sample ID volume Begin End Total Multiplier (mg CaC(WsL)
((S -D) /[(S+D)t2]J x 100 number (ml) ml ml ml go-S7.!VA. 2.0 D
_40-"
Dulicate (B) 4, O'
1$j D
A Ma&&i spiake recoer:_____
olerence standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CmCO*)
(m*
mi ml ml
-4__-_
_o S_
II.S zu C._
Sample hardness (B)
Measured spike value (MV)
%R=MV/SVxIOO.
(Ing CaC03/L)
MV= A -B (accptAble range (m
CACOiL) 75 to 125%)
Sample rneazurenients Sample volume Begin End Total Hardness Sample number Sample I!D (ml) ml ml ml Multiplier (mIn CaCOL)
TV ND Blank (should-Oe 0mgCaCO__)..
A
.0_ 1
-o Vo.o 06.0614 2
-1.*,a. - 4Li&
24.1 -U 1*._.
Os.~,XNf3 3S.o '4-1.
05 Ib
,Lj
- 1. 4 Note: If >15ml of timt is used, sample must be diluted.
Reviewed by. LZ~ZZ Daft reviedwed 1 1 4 SOP C7 - Exhibit C7.I. revision 01-01-13 Page 77 of 117
'Ipr IA r
0 rw*mw~tisan 5Umwv ww Page of Time initiated Time completed o
Analyst [ III Date analyzedL11 ŽiI Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO,/L I Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.-/E (N x 50000)50. ml sample number number (E)
(aceeptable range = 0.0180 -0.0220)
=,Nx 1000 I-IITta utiIe (gCCiiL I (acpal ag Laboratory conml standard."
Reorence standard True value Sample Hardness (MV)
% RS - MV I TV x I00 number (TV) volume Begin End Total Multiplier (ing CaC03L)
(acceptable range (mg CaCOi/L)
- Iml, ml ml
-9to 110%)
1 40 5 O I.0 IC.I
- 2.
"2o.0 U-tC6,,of7c, Duplicate sax pe preciion:
Sample Hardness
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mug CeCO,/L)
({S -D) /[(S+D)/2]) x 100 number (ml),
ml ml ml kc*d.96
-4n,
- 'e, s.o w.(#._ 7-7_
_oo s
UI D*,cf (B)
D t *t-.44 Matix spike reco 1-M Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg C&COsIL)
(Ma CaCOL)
(ml) ml ml ml
- eMC, 9o 1~
~ I So'(.o &dJ2. 20-0 1_
Sample hardness (B)
Measured spike value (MV)
% R = MV/ SV 1I00 (uug CaCOa/L)
I MV = A - B
'(acceptable range (ag CaC22 )
75.t Z
- s.
J)
Sawne measuremets:_
Sample volume Begin End Total Elardness Sample number Sample ID (Ml) ml ml Mnl Multiplier (Mg CCO3 1L)
=(should be- 0 mg CaOO3/L)
AA oio-
%,-4%o~ 0. 4 4...
,.z.
2
.Z2-t,
- e.
621-P__
L_ 7-1_
-4so~
1.-L_
j r460.1
(#,0J*t 4
3
~
1i '%AL Note: If >1 Snl of tilrant is used, sample must be diliued.
Reviewed byr i
Date reviewed SOP C7 - Exh*bit C7.1. revision 01-01-13 Page 78 of 117
H~ardness (SM 2340 C-1997)
RL =.1.0mg CaCO31 Analyst Date analyzd F
ThreMaw nornaii wand muflotipir de&7deabadoa:
Page 40 pare_3of 3 Time initiated [ZZ I Time CoMpletedLIIiii-"
[
Titraut Normality check Begin Eud Total Normality (N) of EDTA ph Fadctor or Multiplier reference standard mi ml ml
= 0.2/B (N x 50000)/50 ml sample number number (B)
(acceptable range- 0.0180 - 0.0220)
=Nx 1000 oS-11-1%4 Mari spike rmewcov:
number (SV) volume Begin End Total Multiplier (mg CBCOz/L)
(ft jNoIA)
(0L) ml ml ml (Ms.t~azs l 25 154
.Dq S L%2 o M4*D3cr Sample hardness (B)
Measured spilke value (MV)
%RMVI SVxI00 (Mg CaCOs/L)
MV = A - B (acceeptable range (MRg Cbcom) u 7a to 125%)
sampt measrmeo.:...
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml
,ml Multiplier (mg CBCO/L)
TV=-ND Blank (should be - 0 n-- CaCO. L) 614 6W.
A LZ
- 5. 9 o
Loo 4~2
..Z -5.
It Ok 4.~
7-f 1SU~.2 Note: If >15ml oftitftnt Is used, sample must be diluted.
Reviewed by: [1.
ýL Date reviewed [106 o 1.1 i-_]
SOP C7-Exhibit C7.1. revision 01-01-13 Page 79 of 117
b0 ~
iU~n SUUSIl Page.
of Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3/L Analst VL,I Date-analyzed [S j S I L l7&ant
.oml~
and dnk~ldeienniadorn Time initiated Time completed t jjto Titrant Normality check Begin EAd Total Normality (M) of EDTA pH Factor or Multiplier reference standard ml ml 101
- 0.2/E (N x 50000Y50 ml sample number umber)
(acceptable range= 0.0180 -0.W0)
-Nx 1000 2
a0.0 t.o 1.0 O.ZOoo Laborator conrol standard.:
Reference standard True value Sample Hardness (MV)
%RS=MV/TVx100 number (TV) volume Begin End Total Multiplier (mig CaCDA/L)
(acceptable range (rag CCOs/L)
(mr ml mi ml
=90tol110%)
J-iG123':4.
40 50 10,0 M'O 7,0 4,tI0 JO,*
Dwftiate le rec~son:
Sample Hardness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (ag CaCOSL)
{(S - D) /[(S+D)1]}x s1O0 number
-WAL..
nmi l
jul jul ad
-ý I
Mairk w/,t rweeo,,.
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mug CaCO3 L),
(Mg CaC
)
/L)
(011 n ml ml
-al ii:)
Sample hardness (B)
Measured spike value (MV)
%R=MV/SVx 100 (mg CnCO3L)
MV=An-a B (acceptable range (uag aoWN) 75s to 1256)
Sample measurements.:
Sample volume Begin End Total Hardness Sample number Sample ID Im) ml ml ml Multiplier (019 CaCO/L)
TV=ND Blank (should be- 0mg_*0 o_
0.0 0.0 0.0 LO fU 0 I
f I
o.u 3
- f.
9 12 Osive-t' A
___-4.1 1i2.7 4A__
Os,.64-*.
Z -t.L
- 1It, 4-%
1_
,s[/
mP4h 1.-0
_4.,,0_
Note: f >1Umi of titoant is used, sample must be diluted.
Reviewed by: I I
Date reviewed I Os" 1Ib SOP C7 -Exhibit C7.1. revision 01-01-13 Page 80 of 117
SET Page. 22 -of S 1
Hardness (SM 2340 C-1997)
RL 1.0 mg CaCO3/L Analystm[d n7 Date analyzedLii I Thrwat iaormalif and muft~lkr detenninadon:
Time initiated Time completed Titrant Normality ebeck Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E
= (N x 50000)/50 m! sample
- n. n mhn n
I I
I 1t&0l 1h h r,
0. 011 R
- 1h A, ",J U -
1 I I
Laboratory control stndad.-
Reference standard True value sample Hardness (MV)
% RS = MV / TV x 1O0 number (TV) volume Begin End
- Total Multiplier (Mig CaCOd/L)
(acceptable range I
(Ig CaCO$/L)
(l ml ml ml I
I
-901 110%)
I I %- I~
40
- 101j, 5L -~
o a
I~
loa%
cbn Du t&Ar ple prvii son:
Sample Hardness
%EPD Sample Sample ED volume Begin End Total Multiplier (mg CaCO3/L)
)
number I
A ml" ml ml S-___x O
StIaftP O 3=.
Ito-w*_)
.,.,'%D Mpli4-03)11 400 1 At________
Mat, sp/ie esreme Rf erece standard ISpike value Sample Siehrns A number (BY) volume Begin End Total Multiplier (ug CDCO'L)
Sample hardness (B)
Measured spike value (MV) r
=MV SVx 100 (g
CaCO)
MVA-1B (acceptable range I
(ma CaCO,/L)
=75 In125%)
IID Sam1ple measwreif~nents:________
Sample volume Begin End Total Hardness Sample number Sample ED (MO ml 011 ml I Multiplier (MR CaC j/L TV=ND (sbould be -0mg QaPO/L)
Ai________q m~tsa
-VA Sao So
~
.~~
i.ok O2 3&s VA_ &W i 3.1 10_
2h
~ 1A J4.4 s
11.'t 3Ž.4 VI_
A 06O.~
1-
.3ZiZ1A3. a Note: If >I5ml of tidant is used, sample must be dluted.
Reviewed by.
Date reviewed [
%k. iZ]
SOP C7 -Exhibit C7.1. revision 01-01-13 Page 81 of 117
U-..
Iii.
Iii F::i":"
.-.I
- I.
Ii I
- ETS 0
i-ae Page 3 f
Of Time initiated [o7 7
I Time completedLIIII1 Analyst []
Date analyzed
,r.*.-,
Sn*,n, anflm
. uI.mmnIna.
Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3/L Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard al nlmlnI l./E 0
(N x 50000)/50 ml sample number number
.(E)
I (acceptable range = 0.01890 - 0.0220)
- NI 1000 Reference standard True value Sample Hardness (MV)
%RS=MV/TVx 100 number (T) volume Begin En.d Total Multiplier (mg CaCOs/L)
(acceptable range (mgCaCOs/L)
(and) ml ni ni l
- 90 to 110%)
I Dm a
e r
sax v pe
-rec-sion:
Sample Hardness
%RPD=
Sample
- ample ID volume Begin End Total Multiplier (mg CaCODsL)
I(S -ID) /(S-D)f2I} x 100 number
-OAA (ml) ml ml ml
... 9 D.
2..% :
Sample measaremes:
Sample volume Begin End Total Hardness Sample number-Sample ID
,(l) ml ml ml Multiplier (HarCCOeL)
TV=
B*
Blank (should be=
n CaO )
=_
-mg_
MwV.A6-,z3 1__
-6 i>, -Ijj i'cAom.O1 s
C4EL~24 -3
?.&0 12A S
_Ia-s-X 3CS
______-3 DI"S 5
11 L', Osd' ot W
1.42~
Ils- -.
Note: if >15ml of fit-at is used, sample must be diluted.
Reviewed by:
J Date reviewed 1 6s I--
SOP C7 - Exbbit C7.1. revision 01-01-13 Page 82 of 117
ES Page Page ___ýo S Hardness (SM 2340 C-1997)
. RL = 1.0 mg CaCO3-L Analyst A Date analyzed 0S~j1,4 ]
Time initiated Time completed Laborator conmb stmadad_
Reference standard True value Sample Hardness (MV)
% RS - MV I TV z 1001 number 0 (TV) volume Begin End Total Multiplier (nag CaCO3IL)
(acceptable range (Mag CaCOs/.)
(00 ml m
= 90 to 110%)
40 50 15-Li' Z.O oo ucate sam e precision:
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (rag CaC*OL)
{(S -D) 4(S+D)t2]} x 100 number (161) ml ml el Duplicate (B)
D______A U
.1 1 W
N k Matrix s"vke Fe*o:rj Reference standard Spik~e value.
Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Iag CaCO3/L)
-Ong
,t (ml) ml mi ml Sample hardness (B)
Measured spike value (MV)
%R=MV/SVx 100 (mag CaCO3/L)
MV=A (acceptable ramp (ma t=
75 to 1 5%) I Sawte measurements:
Sample volume Begin End Total Hardness ESanple number Sample D (Ml) ml ml ml Multiplier (ma CaCOsL)
TV = ND
]Blan A
~l,,
(should be - 0 mg CaOO3/L)
%4,,u r, a
&.- I 41.2,5A.
I m
'3
,'A64 o*.oi Ot 1-40*
- 12. (P-0 1 L10
_,.to__s_.
11_,5 A-4 SAD Ito -11Z
- q oS '.o M&
!A +
(1*
7 1 1 2.o
-+.
L* cksof(
up 1
1 2I2<
k oglo.
03,
%O-
-LI.
4 &1.toI.c Aq-01 1'~o%,04.oc VA W k___I 112 M
oet 1 2 A V Note: If zl-mi of tfitrat is used, sample must be dautcd.
Reviewed by: r "j j"
0\\
Date reviewed SOP C7 - Exhibit C7.1. revision 01-01-13 Page 83 of 117
- ET5.
0 mlm*dTs*Sl~,It Page d'r Page 6-of_ __"
Hardness (SM 2340 C-1997)
RL= 1.0 mg CaCO3/L AnteysyteK Dale a~yzed 0
344 -
Time initiated [,
f3 I" Time completed m
Duplikte s lie precision:
Sample Hardness
%RFD f
Sample Sample ID volume Begin End Total Multiplier (Hg CaCrdnL)
(s - D) /(S+D)i2fl X 100 number m
i l1
_j 7,U_
A!9 12.0
~I(S 4.5 M u sCI I uDplio nte (B)
D4O_
spUW reovwry:
Spike value Sample Spike bardness (A) e (SV) volume Begin End Total Multiplier (MgCaC03L)
(rgCaCO3L),
(101) mi ml ml pie hardness (B3)
Measured spike value (MV)
%R MV/SVxlO j CaCO./L)
MV A - B (scceptable range Sam volum 1 Begin End Total Hardness Sample number Sample IDi ml ml ml Multiplier (mix CQaCOJL TV ND Blank I___N (should be 0 gig o n 2L/C N.)
Note: Vf >15m] of titrAt is usedl, sufnpie must be diuted.
Reviewed by:[i I ]
Date reviewed 1L5 SiMiI]ý
. SOP C7 - Exhibit C7.1. revision 01-01-13 Page 84 of 117
Page Page __L of L2 "ETS 1
EnvlwunmwtafTehdnpg Sutlm*hnm.
'1 AnalystI ZI Date analyzed.-
%Cjj Iotal Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent: [ ImWio_
Acid reagent: I itI-i I]
Calibration:
0.10 mgEL 1.00 mgL I
Slope "Reference standard number of>
ts a
.s dc Note: For samples with a residual chlorine of > 1.0 m&11, the samples must be diluted to be within the calibration range.
.Laboratory control standarfr&
Reference standard True value (TV)
Measured value (MV)
% RS =MV /TV x 10 enumber (1(g/L)
(acceptable range = 90 to 110%)
I f 0.50 C& 01 DUPlicate sw 1*recia on:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = ((S - D) /f(S+D)/21) x 100 number J(mi/L (acceptable raage -:h 10%)
4 Duplicate D z_,01kA Samlemesuremnents:______
Sample Sample ED)
Sample characteristics Reesidual chlorine number 9n ReagentBlank
_0 00643 ILI)4gQA-oZ So.i4 ~w-0
_COW(PZ IV o
t Laboratod control standard:
1140 (9.0
(
N 04...J (r
41 "e av eepalA ra0 -= %110%)
~I
=
o odt' Reviewed by I P-Z Date reviewed C pf t*
SOP C8 - Exhibit C8.1, revision 01-01-13 Page 85 of 117
Page of_
40:ETS EnvironmeAn Tnting Soiuft n hIw.
Analystion:I]
Date analyzed [
~
q Calibration:
rotal Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter Accwumet Model AR25 pH/Ion Meter Iodide reagent:
Acid reagent 1111111111
-=1111W
.0.10.mgL 1
1.00 mil Slope I Reference standard number I
I lo-o.-d Note: For samples with a residual chlorine of > 1.0 mg/L. the samples must be diluted to be within the calibration range.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS =MV1ITV *100*
number (mg/L)
(rag/L)
(acceptable range - 90 to 110%)
- 1.
Dup11cate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD-- ((S-D) /[(S+D)/21) x 100 number (mglL)
(acceptable range -mL 10%)
Duplicate D"MLO,ocDLo At3l Same measurements:
Sample Sample ID Sample characteristics Residual chlorine number I
Reagent Blank os_
iupato
'W0e-bank PAU(O 4
L*cvr-140SZ)6,11~4 qrvv U
1409(0.,.-13 Paes c1MW*.L,.o0
.0o.?o 2
,.N____-_
09->.k Note: All samples wee analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
LaboratorP control standar" Reference standard True value (TV)
Measured value (MV)
% RS =MV I TV x 100 number (nag/L)
(Mng/L)
(acceptable range = 90 to 110%)
0.50 0.o 0.
Reviewed byI Date reviewed LO___
ioZj SOP C8 -Exhibit C8.1, revision 01-01-13 Page 86.of 117
Page 5 Page.L. of__L.
9 S
S S
f~ Izw~mmua,,tdTsstIng Salmtiug.I,,c.
Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0. 0 mg/L Meter: Acurnet Model AR25 pH/Ion Meter Date analyzed Calibration:
Iodidereagent 4Ne wl.
Acid reagent: [~ŽIZMZJ 0.10 I 1.00 Img/L Slope I
Reference standard number C
ZUSS 1t - *5.-0 %m Note: For samples with a residual chlorine of > 1.0 mg&L, the samples must be diluted to be within the calibration range.
Laboratory control slandard&
Reference standard True value (UV)
Measured value (MV)
%RS=MV/TVx 100 number (11g/L) r(mg/L)
(acceptable range-90 to 110%/9)
S KWqtý 0.50 O.'z qq.q Duplicate sample precusion:
Sample Sample ID Sample characteristies Residual chlorine
%RPD = {(S - D) /[(S+D)/2I} x 100 number I
I (mML)
(acceptable range -L 10%).
Duplicate Sample measurementi Sa*ple Sample ID Sample characteristics Residual chlorine wi*naber (ml 1/2!M Raent Blank LO.0O 41
.k CAM-*-
A*
sa
.C Lo.oo5%
Crc Ck.Q vqJILJA LO-00101
_A C*ACM 0,
Z.O.oo424'c 14 OSO.A Ca-TVAý pCe.otpA=
00%
LOYz2
'~~otasy-k TJ\\ CU c 1 Qr Cxolor rneav-ma a I-O-Ol42 NotW: Al) samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory con*ol stadardd..__-
Reference standard True value (TV)
Measured value (MV)
% RS MV/TV x 100 1 number (7-gL)
(mg/L)
(acceptable range = 90 to 110%)
lx=IJSS IM, 0.50 I 0.SOS Inn lot" I.
I-Reviewed by Date reviewed as -iOcLA2l.
SOP C8 - Exhibit CS.1, revision 01-01-13 Page 87 of 117
Page (a
Page__?_ of 7.-
F Ernvbu4vneataITe~tft~g 51w1ar2,,lflc.
Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 p1-/1on Meter Analyst [I P,3Z.Z
]
Date analyzed 9;7 Callraion:
I(odide reagent:
Acid reagent: LIIIIl.'i I
0.10 mIL I
1.00 mg/L I
Slope oReerence standard number I
I Note: For samples with a residual chlorine of > 1.0 mnJL, the samples must be diluted to be within thec calibration range.
Dulicate sample precision:
Sample Sample IOD Sample characteristics Residual chlorine
%RPD = {(S -D) I(S+D)12j)X1 0 numberC
?&(M (acceptable range~ 10%)
4-Duplicate
_i.O.oO(AjCj Samle measurements:
Sample Sample ID Sample characteristics Residual chlorine number II (MRIL) t eagent Blank
- P 6J sA lo~ke o g jC ger-RO iIS LO.Oamn.
%.Amot. o OXI
' qs~goe, f\\
- e a
o x
Lo.o AoS6 14 O. Vb WMtO "In-Otj*/,
-no czcoýr 9.&okr L-otoo?.'H
'4o~ctb TL.-
sA*ro La.oo too NOS&i.IS N 0 Ae sýC ijoý_e speWo LO.000i11 Nove: All samples were analyzed in excess of EPA recommended holding time (i5 minutes) unless otherwise noted.
Laboratory control standard;t Reference standard True value (TV)
Measured value (MV)
% RS MV /V x 100 number (mgL).
(Wg/L)
(acceptable range = 90 to 110%)
LU0.50 0.15 o 7, I
Reviewed by [
I I
Date reviewed 6S-08"1#4 SOP CS - Exhibit C8.1, revision 01-01-13 Page 88 of 117
- Envtiumwu,,tTesng Sahztlan& Inc.
Page 1,of
'L Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accmnet Model AR25 pH/lon Meter Analyst [7777j7 Date analyzed 05Et-j I Iodide reagent [ j3 Acid reagent; [i*K q7I Calibration:
0.10 mg./L 1.00ma/L I
Slope I
FlReference standard number It's%
\\\\.
M% 1%-G
-I q...
I Note: For samples with a iesidual chorine of > 1.0 mgiL4 the samples must be diluted to be within the calibration range.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 1
number (1ng/L)
(mgL)
(acceptable range = 90 to 110%)0)
Ki*\\ -L 0.50 D~uplicate sak mle eilon:
Sample Sample 0D Sample characteristics Residual chlorine
%RPD = [(S - D) /[(S+D)/21) x 100 number
)(acceptable range =: 10%)
Duplicate D e Sant, measurements:
Sample Sample ID Sample cbaracterlsties Residual chlorine number Reagent Blank Gr Ag1~
.).-
ATTA14O06A 0__
%,%66wob cgiL UUa~ntj
~j L0,00360 MotR All samples were analyzed in excess of EA recommeVed eold se time (15 minutes) es s
n Laboratory control standard:
Reference standard True value CM)
Measured value (MWV)
% R.SMV /TV x100 I number (mg/L)
(rmg/L)
(acceptable range -90 to 110%) j
- Mlb, 0.50 J-*4 oo o
2 L" Reviewed by V II IZ Date reviewed 1 1L SOP C8 - Exhibit C8.1, revision 01-01-13 Page 89 of 117
Page 2-of 9 SET SEmvnhnmno"TItUing Solution* InQ Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst
-I I41 Date analyzed OS-j 45 jJ R(odide reagent:
Acid reagent:
Calibration:
.0.10 mg/L l
1.00 mg/L Slope I
Referece andard number Note: For samples with'a residual chlorine of > 1.0 mgfL, the samples must be diluted to be within the calibration range.
Dinlicate samoie Precisiona:
Sample number Sample ID I Sample characteristics Residual chlorine I
Mu]Ll I-IN#, Isola lak1f)OW Du'plicate D to, 001510 I
Sammie meaua'renents:
Sample Sample ID Sample characteristics Residual chlorine number
.mK_
L
-egnt Blank ACl CC-.
I %A Sib. IQ 10A
~
~
0-003_
A2 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 number (rag/L)
(ing/L)
(acceptable range -90 to 110%)
0a.I5017_
q 1.n9,
)-N Reviewed by [
AIT Date reviewed L S2:I)" M SOP C8 - Exhibit C8.1, revision 01-01-13 Page 90 of 117
Sequoyah Nuclear Plant Biomonitoring May 06 - 13, 2014 Appendix D Reference Toxicant Test and Control Chart Page 91 of 117
I I
I I
I I
I I
.1 i
I 0
Pimephales promelos TChronic Reference Toxicant Control Chart E
T Organism Source: Aquatox, Inc.
Environmental Testing Solutions, Inc.
1.0 0.9 0.8 0.7 0.6 USEPA Control Limits (+ 2 Standard Deviations)
I.
I I
I I
I I
I I
I I
I I
I
- 0.5 1.4 1.2 U
VU A5 0.8 0.6 0.4 1.4 1.2 J
I I
I JI IJ I
I I
I IJ I
I I
I I
I USEPA Warning and Control Limits (75th and 90e Percentile CVs)
-J I
I I
1.0 I
I I
I I
I I
I I
I I
I I
I i
I Lborator Warning and Control Limits (1 0 th and 25th Percentile CVs) 11
-I
" I, I
I I
I I
.I I
I
-I 0.8 0.6 0.4 Test date 0
7-day IC25 =-25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC2 5)
Warning Limits (mean IC2 5 *SAO or SA.75)
,psdhet.
Control Limits (mean IC2 5 +/- SA.255 SA.90, or 2 Standard Deviations)
Rk
,ir.
R.
e d.
Page 92 of 117
EtTS En~twmuuanwtl Teau* ngscitmus, Inc.
Tedt anhb Test date i-a111 au a
k/LW k/Q LW Pimephales promelas Chronic Reference Toxicant Control Chart Stand USEPA Lobo taoiy S
LAnsF Units 3SAM Wun*ug lmits cr-a ca+zs Ca-$
CrT+5AJ SA-3t caanaiumIts CrSSAD ClrUSta a-Se, CrSI S,.*m Warrnve Limits Cr - SL7-3 Cr+".-
UEPA SAM Ccotrl Lim ts C-r -M C+ SAM Cv 1
2 3
4 5
6 7
8 9
20 11 12 13 14 13 17 19 2O 02-05-13 0GA 0.72 05 0.62 au 0,09 0.4 03-05-13 0M74 0.72 0.05 0.62 0.83 039 0.64 03-05-13 0.79 0.73
.0.45 0.63 0.83 0.09 0.64 04-02-13 0.77 0,74 0.O5 0.64 0.83 OUR 06S 05-07-13 0.63 0.73 0.05.
0.62 0.4 03 0 D0.4 05-14-L3 0.78 0.73 0.07 0.59 0.K6 0.09 0.64 06-1-13 0.73 0.73 0.04 0.63 0.84 0.39 0.65 07-W0-*3 0.74 0.73 0,05 0.63 0.64 0,m 0.65 0846-13 0A8 0.73 0.05 0.62 0.84 0.0 0.64 09-10-13 0,83 0.73 0.06 0.82 0.85 OS M
09-17-13 0W71 0.73 006 0.61 am we8 0.84 10436-13 0.72 0.73 0.08 0.62 0.8S 0,U9 08A 11-05-13
" 0.76 0.73 0.06 0.62 0,84 0.09 0.64 12-13-13 0.79 0.73 0.05 0.62 0.84 0.8 0.64 01-07-14 0.81 0.74 0.0" 0.63 0.25 0.09 0M65 02-04-14 0.71 0.74 0AS 0.63 0.85 0.09 0.65 W-211-14 0.84 0.7 0.05 0.64 0.LU OS OS 03-11 0.79 0.75 0,0s 0.54 0.3G OS 0.66 04408-14 0.76 0.75
. 0.06 0.64 0.8S 0AS 0.58 05-05-14 0.82 0.75 0.06 0.64 0.87 0309 0.6 0.51 0.81 0.82 0.82 0.81 0.82 0.82 0.82 0.32 0.82 0.82 0.83 0.83 0.84 0.84 0284 0.84 0.84
- 0AS, 0.15 0.1S 0.15 0.15 0.1.5 0.15 045 0.15 0.15 0.L5 0.15 0.13 0.15 0.16 0.16 0.16 0-16 O.S7 0.57 0,58 0.37 0.58 058 0.57 O.58 038 0aB O..5B 0.59 0.59 O.S9 O.59 03L8 0.28 0.88 0.28 0.83 0.28 0.9 0.28 0.g 0.28 0.88 0.28 0.89 0.28 0.55 0.28 0.88 0.28 0.35 0.28 0.89 0.28 0.89 0.28 0.8 O.28 0.88 028 0.85 0.28 O,89 0.28 0.89 a.28 0.90 0.28 0.90 O.28 0.91 0.25 0.91 0.29 0,45 O.45 0AS 0.4 0AS 0.45 OA45 OAS 0AS 0.45 OAS 0A6 0,46 0A6 0.46 0.47 1.00 0.33 0.40 1.00 0.33 0.40 1.01 0.33 0.40 L.M2 033 0.4O 1.02 0os 0A4 1,00 OM 0.40 1.01 03 0.40 S
0.33 0A0 1.01 0.33 0.40 1.01 0.33 0.40 3.01 0-.3 O.4O 1.01 0.M3 0.40 1.01 0.33 O40 1.01
.33 0A0 O2 0.33 0.41 1LM 0.33 0.1 10 0.34 0A1 1Ji3 d.34 0.A1 1.03 0.34 OA1 1.04 0.34 0A2 Los3 0.07 1.05 0.07 1.6 0.07 1.07 0.07 1,06 0.07 1.0A 0.07 1.06 0.06 1.06 0.08 1.06 0.08 1.06 0.07 1.07 0.07 L07 0.07 1.08 0117 3.06 0.07 LOS 0.0J7 Nc 7-d C.s = 7-<by M55 nhibitlon concntorUon. An astmatin ofe concentraion of potassium chklode that wAd ca*e a 25% reAction In Plmnphals growth for th, Lus potnttIo CT ral tendency (menn s. }.
S-Slandard dvitin of the ICavalUes.
Laboratory Caonrol and Warhig Lbm.ts Laboramvbr cntml and wmntn rimlts were eablished irn the standard devation afta lCvahaas I
rr** pondngt Owlta 10th and 23th p*rrelCL Thaesa range are mnor sringent than tha cwousS and warning Oirndts recommended by USM fra the test method and endpoint.
Sas.= Standard daviatlon mcespandig to the Iu paernfile CV. (5s,=0.12)
Sm= Standard viAwtla cor sporid~ngthtie 25!h percm a CV. (5nA -
.213 SEWA (.eal a*l Waa*ig
-M I
.*=
Standard deaiation wrespondtng to the 75 percerle CV. (S,, a 0.38)
$n= Standarddaviatton cmespondngtodtheag0thmantlia CV. (Lea 0.43)
CV= Coaffldatwubfarblaonoftha tclalues.
FPg p93 o0f-11147ss Page 93 oF 117
Pimephales promelos Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements evlr*onmi.*tlTsng Soluions, Organism Source: Aquatox, Inc.
1.25 I
I I
I I
I I
1.00 0.75 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
I I
1 I
I I
I I
I I
I I
I I
I I
I I,
I I
I I
I 1
i I
I I
I I
I I
I I
I I
30 Kentucky Acceptance Limit 30.0%)
Ic 20 10 s*
- . ÷,.*
W 0
- ---o,.,
__I oto Rerdion Iofldn f
a iato (
I) or Peren M iniu Siiifn Diffeen 30 30 I
I (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
,,*/.,
Central Tendency (mean Control Growth, CV, or PMSD)
Control Lmits (mean Control Growth, CV, or PMSD +/-o2 Standard Deviations)
Gnripsupernstgdtmrm asndlded x.ini apmreadheet.
Exe.pe~et~rntm d y:. J. S~mI~r bvIk-Ad.b Page 94 of 117
E TPrecision of Endpoint Measurements EPimephales promelas
$(*vbamn.tMaeebI Chronic Reference Toxicant Data Testnumber Testdam Control survival Control Mean Groffwth Cr CV Ca WoD PnID ar for Control Growth K)
(n/tleme)
(mn/Ilrvae)
(Vol for Contro Growth CV (9)
(%)
for PMSD (961 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 02-05-13 100 03-05-13 100 03-05-13 100 04-02-13 100 05-07-13 100 05-14-13 100 06-11-13 100 07-09-13 100 08-06-13 100 09-10-13 100 09-17-13 100 10-08-13 100 11-05-13 100 12-03-13 100 01-07-14 100 02-04-14 100 03-11-14 100 03-11-14 100 04-08-14 100 05-06-14 100 0A87 0.504 0.564 0.582 0.651 0.599 0.635 0.615 0.666 0.538 0.594 0.679 0.674 0.576 0.637 0.716 0.666 0.689 0.717 0.635 0.495 0.518 0.534 0.557 0.564 0.574 0.579 0.589 0.584 0.585 0.593 0.599 0.597 0U600 0.607 0.611 0.615 0.620 0.621 7.4 8.5 9.1 6.5 7.8 5.7 5.5 49 8.3 6.1 4.7 10.2 15.7 15.0 9.0 6.2 8.1 5.8 10.8 15.9 7-9 8.3 7-9 7.8 7.5 7.2 6.9 7.1 7.0 6.8 7.1 7.7 8.2 8.3 8.2 8.2 8.0 8.2 8.6 0.06 12.8 0.07 14.7 0.10 16.9 0.12 19.9 0.08 11.9 0.08 12.8 0.06 9.2 0.09 14.5 0.08 11A 0.06 11.8 0.10 16.2 0.09 12.6 0.11 16.6 0.09 15.0 0.06 9.8 0.08 10.9 0.08 11.3 0.07 10.1 0.11 15.3 0.11 18.0 13.7 14.8 16.1 15.2 14.8 14.0 14.1 13.8 13.6 13.8 13.7 14.0 14.0 13,8 13.6 13.4 13.3 13A 13.6 Note:
CV - Cofficient of variation for control growth.
Lower CV bound determined by USEPA (1e percentile) = 3.S%.
Upper CV bound determined by USEPA (901 percentile) - 20%
MSD - Minimum Slignifcant Difference PMSD - Percent Minimum Significant Difference PM5D Is a measure of test preclslbn. The PMSD I the minimum percent d*fferance between the cmntrol elnd treatment that can be declared statistically significant In a whole effluent toxldty test Lower PMSD bound determined by USEPA (11e percentile) = 12%9 Upper PMSD bound determined by USEPA (90t" percentile) = 30%.
Ca - Central Tendency (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toidclty Applications Under the Naitfonasl Pollutant Discharge ElI*ination Program.
EPA-83340-0CS. US Environmental Protection Agency, Cincinnati, ON.
USEPA. 20Ga, 2001b. FInal Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix EPA421-B-014.104 and EPA-421-B,.01-05. US Environmental ProtecnAgency, CincInnat], OH.
bFft ppkIocr_05.06-l4*zasx Entered by.. SlmmUi Reviewed by:;
Page 95 of 117
IV.I I
I I
g I
I I
1 I
1 I
'ETS Page 1 of 6 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: PmeWhalde promelas PpKCICR Test Number :
Diuton preparation
,mao:..
Comen:
KC1 Stock INSS number.
t9'c V"'T" Stock preparation:
50 g KMCL
__________________DMýWw~ce 5zKOin-M1n111lt.
Dilution pr (mg/L) 300 450 600 750 900 10o0 Stock volume (mL) 6 9
12 15 819 21 Dilueat vohne (mL) 994 991 988 985 982 979 Total volume (mL) 1000 1000 1O00 1000 1000 1000 Test organism informaion:
Test information:
Organism age:
IA.Wm y
- z i#
s &or Randomizingtemplate:
St.
- Date and times organisms were
,(-t
-ro "T
O" Incubator number and born between:
shelf locatiom:
Organism source:
A h
p6-MA A Pip aS-OSt larenita CHM nimber:
CAK
-0 Drying information for weight determination:
Transfer vessel information:
pH I ". as S.U.
Date / Time in oven:
Temperature P'1 C
Intial oven temperature:
Average uasfer volume.
0-Date/Time out of oven
- N.r i-%
Final oven tnemerature:
Total drymgtime:
Daily feeding and renewal informalon:
Day Dabe Morning feeding Afternoon feeding Test Initiatio, renewal,'
MHSW or termination batch usfed Time Time An~st
'he Anls
-60 itAna-h C
n-avoo AL moo A4 l
'1Sq L
o-2 0S. otN
- m.
mg A(
LOMS
)4d GS-CtnP 3_
d%-m i
nb tso A4
~
~
~
.L
~i 5
os,* '4 M K, v
rc-ib -4 A M V m fl....
- l m
m -- W 6
7 O~- ii. N OSd%416%i I
I I
Acceptce crit.ia S m a of,rsp n.
t:
- 07.
- 0%
7-day LCo.
t.
Avere wet Per initial larvae:
0, NORC AvmV wýeht per surviving larvae:
I snuj5ariae LOEC I ChV brfw by flObY L Xw SOP AT21 - Exhibit AT21.1, revision 06-01-11 Page 96 of 117
0S
'S Page 2 of 6 Species: PhiMphalks promelw PpKCICR Test Number:
-WI-Surival and Growth Data
[
Day Control 300 a KCI/L 450 mi KCI/L A
B.C D
I F
G IH I
J K
'L 0
10 10 10 /0 1, 1
1 i
/o
/0110 /0
/0
.2 I
10/01()1.
1/0 1Q1001(
10 I)i)10 2
~~10. it)o 1 -0 !t 1 0 i t)
/C 10.
ID 1011.0 101 3__
1
°)
IC) 10 (1,0
,( 0 IC) 10 1 0 to (0
610
- 10. /o 10 I")"
W 0 10 1'0 1'0 5
b t..
o Mo
,ot,o o,to, 10 1
,o 0.1,c to 6:
it), IDo t*o lr I, ) 10~
too
,0
/0,0 o 10* to
- 1) 0o 10o to to
,o to
,o A Pan weght Tray color code::.I
'.?~Z Analynt
ý,
VM 4202 1.,15.43 IS7.t9 bI 53 q5lTz B = Pan +4 Larvae wel~ht (lug)
Analyt VJO Z&4
~%Z2tI 23A5 22."nz o.~.?
Data-06 1-#
O5 21"Z D6B3 5q C-Laryse weIght (ug) -B
- A Handalculte.-.j
.0LO 6IQ Iq Wdgbt per Initial numb'r of larvae.(mg)
-C /IItIal number ef larvae Hand calculated.
Aailyst or_
jrj*
e 07 4b 1%
0 J?
p~7 4
i-I iI Average weiht per Initial Percent number of larvae (o redueton 0. 10 CS from control M
I 9-h mmI ei. -IL
-%V*I.
7 0...
104
-s.t7.
-s I
I -
I I
I I
I Comiment codes: c-clewr,d -dead, fg ingus,
=killedm
-mssing Ak=
sick,sm"m =uusually smull; Ig = unusually large, d&z = decanted and retumed, w =wounded.
CoNMOMe:i bhyL b.s.~
SOP AT21 -EBxhibit A1-21.1, revision 06-01-11 Page 97 of 117
- v.
T Species: P/rn Page 3 of 6 PpKCICR Test Number.2_L-t/a/es prometai Survival and Growth Data I
i I
1 t
I Day 6
mg KC-L ma KCIL 900 m5 KC0L M
N 0
P q
R S
T U
V W
X
/0 /01/0
/0 10 q 0 10
/0 10 to 4
e10
/01 10 o
44A 1,oo io to a
'i e~
8 6
s 1
6o I i to 0
,o
- a.
S 9,(
0 7
10 10 1* fa "1
S
(.,G A P No weight (mag).
Tray color code::
Snesk-
.eew An**.': -
IAO
%0 1101.Ol IkJiL 1,.o12 I joq lIq.gpo16.0 15.3% Iq.6q15*
I
.li,I(J.1
=-Pau+Larvaewe-kht(mg)
Analy,2.
W i5 SS 3.65
,.* o..
,z4fe z.*o 1.q 61 i.
Date:
C = Larvae weight (rag)= B-A Hand calculated.
1i 1,1 "41.
.SI
.9 b,,1. s.Ai &.
Rq LSA
'.W4 Weight per initial number of larvae (mg)
C fltial number of larvae ruen Average wreight per Initial Percent number ofrv Io
. +
7.
ol.aom
(,g.)
O.rcnO oA to.
Comment code:.c = clear, d = dead, fg = fumgus, k = killed, m = missing, sk = sick, am= unusually small, ig - unusually large, dMr - decanted and returned, w = wounded.
i' i
Cobmmants.:
klL VdAU4 SOP AT27 -Exhibit AT21.I, revision 06-01-11 Page 98 of 117
'3 p
I I
I I
I I
I I.
- ETS Page4 of 6 Species: Pneoha/es grormie&E PpKCICR Test Number: __?3-Survival and Growth Data Day 1050 mn'KCI/L Y
Z AA BB i0 i I0 10 I0 /0 2
3 S
55 6
7 7_ A &AI A - Pan welgt (
z)-
Tray color code::
Analýyt
.12 I..4 IJ.
l..5 I66 Date:
O 5......
B - Pan + Larvae weight (mg)
Ny,*
60
.TtI 1O105 lD.91 up,.51 C - Larvae weight (mag)- B - A Hand calculated.
6
- e.
O.
0.
Aualypf Weight per initial Pmber of larvae (meg)
=C/Iial number of larvae r o
Hand calculated.
j bJ.
Analysft:
0' C
Average weight per initial Percent number of larvae (mug)
Ireduction
.0b.
Ifrom control 0
Comment codeso clear, d = ded, fg= fingus, k killed, m - missing, sk sick, sm unusually small, Ig - unusually large, d&r = decanted and returned, w = wounded.
SOP AT2 - Exhbibt A771.1,revision 06-01-11 Page 99 of 117
?II1 e
ETS t* EnvIronmentalTresting on nsI,'
Phuephales promels Chronic Reference Toxiant Test EPA-821-R-2-013, Method 1000.0 ai.floty C otmand Verificatfon of Data Enr, cualclations, and S'tatistical Analyses wiannal 82
.,eth.Sq A-t KSS~m elI~m wahl w lm'ae' (rood m
n nmeaj
[md/
nlurbr
,4
- =mid S 0=0.d*
A-9q 9
fb A
10 10 1o 5 1a 445 0mS 0.4S5 Uomd a
10 10 1632
",v7 r.75
.6"75 oM63 a5.
woo a2 0M15.5 mateppomi.
C 10 1
10 15.26 5
L.89 0.639 aim O
in 10 16.28 23,49 7.31 0.731 0.731-is 10 19 1..3 2317 7.24 0.724 0CU4 Fo 10 10 1 7 iJ 7.02 0.702 0.725
- 5.
o.M loo C725 52
-1&2 o
1O 0
10 1624 23.93 7.79 0.77-M779 06779 H
10 10 1522 22.77 6.95 0m 0.65 I
10 10 1.14 23.v 7.13 0.713 0.713 40 J
110 1
14.59 23O s0i0 01504 10O 0Osn am 668
=6
-S.1 S
1 O
15.23 2
LID 6.0
,E M0.610 L
10 10 1.50 253M 7.48 0.743
__43 a
10 10 1w"0 --
.56 6.73 0576 a.i76 N
10 10 15.01 23.48 7.47 0.747 0-747 so,
9,.
9;0 in 20 -
im 7.22 OJ7I2 GV 3o.7 DG ae P
10 10 0
22._
.M16 E&04 S
10 a
M 22.22 m
oL2 10381 am10 7
JA 5p ID,9 0.0_
59
- 0.
0.808 7.3 43 10 9
15.04 22.40 6.42 0.713 0642 1
10 8
13.31.
21.10 649 0M11 O
0.
U 10 56 4.4 38.37 3.73 0.622 0373 v
0 5
15.87 1930 MB 0.778 0.736 0.03M OA4O 9.2 37.1 WA 10 1
15.18 1.951 09 1
0.820 Om.39 8B9 10 1
1I3ss8 16.51 0,.0 0.930 a09,,
Daem mm ntue 0.43M PNL0?
15.0.
fm0-Mhthem UgdftmwA O~etbui Peso P6.Mtiwsftmkk na~kmtvmfewnt PIMs hIa miMmnuft7tpmd$stM. The PN60 tIthe mIibfnm pnmfntfdC hee betwan twew ms* end toubmtmtttiamt bed dacb-6 sflted y diVthei0 a mwMhffio ustfttvta LUMer PLS huid det-mihd by UMPA(Ith pemvIS)
= 2.w U,'one PLIE humir*d detsi'iS by IISPvA (Sipenetlej 30ao2" Leeramd uPper P50 bounds wee d2temlhnd fromthe 10th and 90th pownhtne, rezpecduvly, of PIeat dadt *om EAs E
- WEThflhm Va*bIft %npUS&A, 300w U9. a USWA. S001eo30t. Phdbr-~
Swrt: kflaM:bed Vwalh*Saudy of PA SaerbtmeniiOuuk end Acib*o ut ai5ctTmdt*M~wethdu.V s 1 n pdi 8241C4edWAIS0.
SEdeoolf0o Seensy. Chidine, W Ffb FpWcr_05*.-14.w Pwagd b10 of s1.w kamied Page 100 of 117
0ETS Statistical Analyses la FshGrowth and sJeTut.7 Survival Start Date:
65M2014 Te"t D1 PpK=ICR Sarle ID*
REF-Ref Toxlcant End Date:
511812914 Lab ID. ETS-Fnvlr. Toolng S."
Sarrpe Type:
KCL-PotasSlum chloride Siples Date:
Protocol: FWCHR-EPA-821-R-02-013 Test Spedes:
PP-Plmephales promaela Comments:
ogMn 1.0010 2
1.0 4
D 00 1.0000 1.0000 1.0000 1.0000 40m 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 00 1.0.000 0.0000 1.0000 0.0000 75D 0.8000 0.7000 0.0000 0.8000 9o0 0.6000 0.5000 0.8000 0.5000 1050 0.2000 0.1000 0.1000 0.1000 Tranafotrn Amcaln Squae Root Rank 1-Tailed Number Total Conc-n*
Mmm N-Mean Mean Min. Max CVA N.
Sum.
Crwtleal Rasp Nunber D-Cortrol 1.0000 1.0000 1.4120 1A120 1.4120 0.000 4
0 40 300 1.8000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 0
40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 0
40 600 1.0000 1.0000 1.4120 1A120 1.4120 0.000 4
18.00 10.00 0
40
- 750 0.6000 0.8000 1.1138 0.9912 1.2490 8A78 4
10.00 10.00 a
40
- 900 0.6500 0.5600 0.8367 0.7854 0.9861 6,955.
4 10.00 10.00 18 40 "1050 0.1250 0.1250 0.3572 0.3218 0.4838 19.861 4
10.00 10.00 35 40 Auxiliary Tets Statistic Critical Skew Kurt Shpiro-Wi~l)a Teal idicates non-normal disrbltitlon (p -s 0.01) 0.79852 0.898 0.87848 3.89312 Eualit of vevenoe cannot be coonted thyp
.e.
Ts
(.ai:0.
NOEG.
LOEC ChV.
-"U StBM Many-.One Rank Test 600 750 670.82 Tmmlhner vs D-Contral Maiudmum Llkenhood-Pmblt Parameter Value 8E 95% Fiducal LUmlts Control CMl-Sq Criltlad P-value Mu Samana Iter Slope 14.1020 1.98475 10.2126 17.995 0
2.43897 9A48773 0.8588 2.94900 0.07091 a
iterdcept
-36.69 6.04826 -48.047 -25,134 TSCR 1.0 Point Prob9 nalL 96% Fiduchal Limits
.I ECODI 2.674 80.287 524.399 663.982 0.9 EC00 3.355 679.879 609.654 726.e74 0.1 ECIO 3.710 721.427 659.919 783.3813 0.7.
ECIS 8.964 750.884 696.606 789.076
'EC20 4.158 775.151 724.831 811.863--
0.0 EC26 4.326 796.509 750.356 831.856 0.6-
-EC40 4.747 863296 615.147 888.739 EC00 5.000 889.331 883.2 92.64 0.4 E5C0 6.263 028.889 889.9 973.741 0."
EC75 5,074 992.864 948.801 1080.09 0.2 ECB0 6.842 1020.33 071.673 1098.901 ECOS 6.036 1053.31 098.406 1144.88 0.1 M090 8.282 1096.31 1032.40 1207.04 EC05 6.640 1163.31 1064.14 1307.03 10 100 1000 10000 ECO9 7.326 1-00.23 1188.11 1520.04 Dose mgIL
'oss-Rep Plot h.i.h1 M.y.btmw Mi. ppknite-05-06l4xdsx fthed by, 3. 8 Reviewi b~r x Page 101 of 117
J r!
S a
SETS albffluflebnb&
Staistical Analyses '
Larval Fish Growth and Survival Test-7 Day Growth Start Da*t:
5=8/2014 Test IDt. PpKCICR Sample ID; REF-Ref Toxdcant End Data:
5113/2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potaaalum chloride Sample Date:
Protocol: FWCHR-EPA-321-R-02-013 Teat Specie.:
PP-Pimephalea promelea Comments:
Conc-fntgL 1
2 3
4 D-Control 0.4950 0.8760 0.1390 0.7310 300 0.7240 0.7020 0.7790 0.0060 460 0.7130 0.8040 0.8100 0.7430 600 0.6760 0.7470 0.7220 0.5160 760 0.5010 0.5690 0.6420 0.6490 900
.0.3730 0.3890 0.3820 0.4540 1080 0.1050 0.0540 0.0820 0.0990 Tranuforn: Untranabrnned 1-Talid Ivatonlc Conc-mg/IL Mean N-Men Mean o
Mn Max COVI N
t-Stat Critical MOD Mme N-Men D-CoIrItU 0.6360 1.0000 0.8350 0A960 0.7310 16.861 4
0.8800 1.0000 800 0.7260 1.1417 0.7250 0.0960 0.7790 6.250 4
-1.603 2.290" 0.1143 0.8800 1,0000 450 0.6676 1.0512 0.6675 0.6040 0.7430 10.632 4
-0.681 2.200 0.1143 0.6789 0.9883 600 0.e8O3 1.0570 0.6803 0.6160 0.7470 8.341 4
-1.107 2.290 0.1143 0.8780 0.0983 760 0.8078 0.9571 0.6070 0.8500 0.8400 7.338 4
0.6078 0.8935 900 0.3995 0.6291 0.3995 0.3730 0.4540 9.261 4
0.3998 0.6875 1060 0.1080 0.1009 0.1080 0.0620 0.1680 37.380 4
0.1000 0.1859
.Awd. ny Teats tStatltc Critical Skew. Kut ShA O-Wlk's Teat Indicates normal dlatultlon (p : 0.01) 0.91767 0.844
-0.5432 -0.0326 BaMra Test Indicates equal varances (p = 0,49) 2.43364 11.3449 Hwtheas Teat (1-taffr 0.0 NOEC LOEC ChV TU NSOu FalDS F-PmW W
DunnttfTeast 600
'600 0.11434 0.18000 0,00575 0.00499 0.36788 3,12 Treatments vs D-Control[
Unear Irrtequolftlon (200 Resamplida)
Point mg/L ID 96% CL(Exp)
Skew IC05 609.33 07.44 220.10 801.81 -1288 1010 741.04 62.37 632.17 791.21
-2.5843 IC1S 771.43 21.35' 871.57 608.24 -12924 1.0 IC20 796.92 14,88 739.25 834.36
-0.1298 0.9 IC25 520.41 13.40 709.74 J155.76 0.0084 IC40 603.88 11.69 853.49 918.48 -0.1270 1060 930A1 7.82 903.23 950.17 0.0643 0.7 0.6 0.5 0.*~CA S0.3.
0.2 0.1 0.0
-0.2 0
500 1000 1500 Dose mglL 00seP*aeponee Plot 1-tall, 0.05 level of significanca Ltwsd.M b*w~
thtL beam tin-Pik ppukw..p1.O6-i4 xis BlMWdby. I.Sie Itaviaw by.
Page 102 of 117
I I
I FŽ:.
I I
I I
I I
1 I
I I
I
- ETS Page 5 of 6 Species: PimeDhs promelas PpKC)CR Test Number J2.j*-
kid&V Chemiy wmmmd Day Analyst Coutentratlon Parameter DO (zn/)
CONTROL Conduetivity
- 1-J.-
- Aikafnlty (ma ('sd'AT.fl, I
- Hmardness
- Temperature (C) 9 pH (S.U.)
DO (mgL) 300.g KCfL
-Coductmty fumhcqlrini i
a
- Temperature I
I -
PH (.U.)
r
=
II DO (zng)
.4.
450 msg KCYL C. ugctMty (aimhokni)
- Temnerature ('I I
I I
II I
pIR (S.U.)
DO (,gIL) 6W0 mg KCIIL Conductivity (umhos/cm)
- Temperature (MC)
'W4,0
--Lq I 134.i IL ~
I a
a pH (S.U.)
2.O*o 1.59 G&q 0L4ZI I
DO (ma/l.,'
750 mg KCVL DO (
N Conductivity Ita9~fl
- .z.z......~
'Temperature (M 1.50 I
~
~
pH (s.U.)
pp DO (aL)
-7.q 900 mg KCI/L Comuca!t -
I I
- 'rTimature ¢c)
-_.U U ~
pH (.U.)
IIOS mg KC1IL DO (miL) condu f-(OM-11-7.15 0
- Temperature(C
__TOCK' I Initial IF TempMatuea erM om p
a t the time o
't0sW Initiation, enewal or umhiai*u by the anyst identified in the Daily Renewal Infmnuation table locae on Page 1.
ity End headness perfouned by ft analyst idenfied on the b*ch skeet specfic ft each analysis and
-aflied to this bench s ihee b01 SOP AT'21 -Exhibit A7l21.1, revision 06-1-11 Page 103 of 117
I.
- g.
II.
I ETS Page 6 of 6 Species:
Mhale promelas PpKCICR Test Number:.
UI Day (Analyst identified for each day. perfor*eed M. D.O and conductivity measurements only.'
i M
I I
3 4
I 5
I 6
Analyst F T
Concentration Parameter m CONTROL PH tS.U.)
DO (mgL)
Conductiv*ty
- AlklIWnt
- Hardnes
- Temperatur for)
I I -
I -
.~-
I P
i I i
- Jl pH (S.U.)
~slq.~
t.s~
l -c.i 300 me KCI/L DO (jngL) 1 014 6A I-'S Conductivity Z
(11mboaXWOW I
- Temperature VLS I
- inII.~I~4I I -
II eq
-L'.j z1o 211 a
-. LA pH (s.U.)
n Fkq' 770 1 --T.q1
_- =
if-450 mg KCI/L DO (uOL*)
Conductivity
'Temperature (Ic) ttiO
"'to JD0o iA.t j~ ~L'H 131.1 Ii ii p
II i
-,r-*.-...
-- I II-pHV$S.U.)
-4 Sm 600 mg KCI/L DO (mglL)
Conductivilty
- Temperature rbq.O A
M 0.1 13SO 13RO h p
i -
p ~
ii DH (S.U.)
JPH (S.U.)
a n 126 6ýi 750 mg KCL/L DO (mg/)
Conductivity T
prathosm r
- Temperature J144 1j~
Up 14-1 I 13-PH (S.,U.)
0
%O i.0q 9'
DO (MWL)
I 900 mg KCI/L Conductivity
- Temperature
'"IqAq **.
- t.
q' 1.
PH (S.U.)
DO (rmnL)
Ills 1050 mg KCI/L ConduCvity (ITnhodcm)
'l
- Temperature
'vl
~
L.
~.
M'
-CC)-
Initial Final Initial Final Initial Final Inital Finali7 U
U U
~
C m
/
- ~~at e performed at the tme of test initiation, nmewal or termination by the analyst identified in the Daily Renewal Informaton table located on Page 1.
(bvnl~n~ty and hardness peribmed by the analyst Identified on the bench sheet spedflic for eawh analysis and transcribed to this benoh sheet by:
SOP AT21 - Exhibit AT2n1., revision 06-01-11 Page 104 of 117
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 4 EnvironmentlTemsting Soluions, Inc.
1.14 1.12 r
I I
I i
i i
i mI i
I i
I I
I I
USEPA Control Limits (+/- 2 Standard Deviations) 1.10 1.08 1.06 1.04 1.02
.......... 0..#
..................................... 0.......
z 2.5 2.0 i
I I
J I
I n
I I
I I
I I
I I
I I
I I
USEPA Warning and Control Limits (7*h and 9 0 th Percentile C-Vs) 1.5 oe~eo ooete eeee~o~oe-
-O¢6
- - -oo oe 1.0 0.5 I-
?......
1.4 1.3 1.2 1.1 1.0 0.9 na I
I I
I I
I I
I I
I l
I l
I I
I I I Laboratory Warning and Control Limits (10(1 and 25 Percentile CVs) 0.9.....
I I
I U,"
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC2s)
' Warning Limits (mean IC2,.L S+/- 0 or S,7)
Control Limits (mean IC2. + SAs25 SA.9o, or 2 Standard Deviations)
&b
,.noatud 1f a
,oId eml andsaw PEag 1s05of1T17d by.J SuwlUI Roolwed br.
Page 105 of 117
lETS 6 ~~
~
~estnglne~
"~~* Sollutlon, hIrm Test numter Testdate 7-day ICn CT kWL-NacI WILNa()
Cerlodaphnia dubla Chronic Reference Toxicant Control Chart State arn USWPA S
Coatrol Unhs
,.AM Ca-2S Cr+2S 1
2 3
£ 5
7
£ 10 21 12 13 14 15 17
%11
.9 20 12-06-12 01-06-13 02-05-13 03-05-13 04-O0-13 05-07-13 05-07-13 06-04-13 07-09-13 09-06-13 0&46 09-10-*3 10-08-13 U1-OS-13 12-03-13 01-07-14 02-04-14 0304-14 04-08114 05-06-14 L0O 1.07 1.05 1.05 1.07 I.0 LOS 1705 LOS 1.06 1.05 1.06 LOS 1.06 1.07 1.05 1.05 1.06 0.01 1.04 1.06 0.09 LOS 0.01 1.04 1.08 0.09 1.06 0.01 104 Los 0.am 16 0.01 1.04 1OG 0.0 1.06' 0.01 L04 1.a 0.09 1.06 0.01 104 1.01 0.09 1.0 am 1.04 1.0s 0a0 1.06 0.01 1.04 1.Ds MOD L.(]
0.01 L.O4 21.8 0.03 1.06 0.01 1.04 1.08 0.08 I.M4 0.01 1.04 1.O9 0.08 1.06 0.01 1.04 1.03 0.08 1.06 0.01 1.04 1.03 0.08 1.06 0.a1 L04 1.09 0.08 1.06 0.01 L04 119 0.06 1.05 0-01 1.04 1.06 0.08 LOS 0.01 L04 1.06 0.09 LOS o 001 L04 1.09 0.0 1.06 0.01 1.04 1.0Lo M.O labontoa Warnks Lin' CT -SW 1
Cr + S,0 0.98 1.15 0.98 1.15 0.98 1.15 o.99 21.5 0.98 1.15 0.99 1.16 0am 1.15 0.9 1.15 0-.8 11S 0-98 1.15 038 1.15 0.93 1.15 0.99 1.15 0.98 1.15 0.38 1.15 0_98 1.15 0.98 1.14 0.98 1.1L 0.98 1-1S
.0.98 1.15 Labhat"y S91 ContSurl lmiSt Ct-SA.
CT4SLA USEPA S".
Warning Units Cr"- SAM af + SAIs USEPA Sa " aoe C
U
+ SAM CV 0.10 (2.88.
0.13 0.88 0.18 0.88 0.18 as.
0.19 0188.
0.19 0.88 0.19 O8AS 0.19 0.88 0.18 0a8 0.18 0.88
.118 0.88 0.18 0.8B 0.19 0.89 0.18 08 0.18 0.88 0.18 0.88 0.18 0AS 0.18 0.88 124 1.24.
1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1-24 2.24 L24 124 1.24 0.48 0.48 0AS 0.48 O.48 0.48 0.48 0.48 0AS 0.48 0.48 0.49 0.48 OA48 0.48 O.48 0.48 0.43 GAB ass 0.S8 0.58 0.58 0.59 0.58 0.58 0.58 050
%.58 0.58 0-ss 0.58 D.58 ass S0.56 1.54 L.54 1.54
.1-4 1.54 1.S4 1.54 1-54 1.54 1.54 1.54 1.54 1.54 1.54 154 1.54 2.54 1.54 L%5 0.68 0.40 0.66 0.40 0.68 0.40 0.66 0.40 0.66 0.40 0.65 0.40 0.66 0.40 0.66 0.40 0.56 0.40 0.66 0.40 0.66 0A0 0.66 0.40 0.66 0.40 0.66 D.40 9.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 O.86 0.DA 0.66 0.40 1.72 0.01 1.7Z 0.01 1.72 0.01 1.72 0.01.
1.72 O.01 1.72 0.01 1.72 0a 1.72 0.01 1.72 0.01 1.72 0.01 V.2 n
am 172 a.01 1.72 0.01 1.72 0.01 1-72 0.01.
1.72 0.01 1.72 0.01 1.72 0.01 L72 0601
.72 "I
NOte:
7-d fCu" 7-day2514Inhibrtionconcmstration. An estimation ofthe concentration ofsodlum chloride that would cause a 25% reduction in Cerulophnua reproduction hor thetat popuation.
Cr-Centr u
(*nduwyneanW*,).
S - Standard deviation of the ICw yhlues.
a'atoayComarol and Waneln t.ints tortatryConbrol and warning limbl were estabished usingthe standard devatlon of the valua cowpdato the Wt ad Zt ie On.
conese rms e
rm te otrol and warning ranits recommended by US5PA forata tort method and endpoint S. - Standand devation correspondingto the le perceftfle CV. (%.
0.08)
= Standard deeiation crrespondingto the 2e percentle CV. (Sa, = 0.17)
UWSPA Co*nrol mid Waring Uinfl
=ý Standard deviation corresponding to the 7e perranifl CV. (kLý,0AS)
SAD Standarddfdiatbncorroeswd*ntotheOecfa CV. (SKo=0.62)
CV = Coecent of vanaetia of the tCKvahua.
USEPA. 2000. Undemuland and Accounthag for Method Vallillty In Whore Effluent Toxdicty Appliations Under dhe National Pollutnt Discharige Elim*ination Program. EPA-33-.R-00-00 US Etvironmental Protectan Agency, Cinnatk OlI.
- abmd br. J. Suterme ReP0.*e fy.
Page 1.06 of 11.7
S.ETS EmbnvrnumeWt T4"tngjSkmmIc Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 9
40 35 30 25 20 15 I I j
j I
I I
I "l
I I
1 J
I J
Minimum Acceptance Criteria (> 15.0 offspring per surviving fenale)
I I
I I
I I
I I
I I
I I
I I
I g 40 30 20 10 50 40 I
II I
I I
I J
I I
I I
1 I
I I
I I
'F'I' i
North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
...................................................................e n
.... i.....t I..I I
Iý I I
II
,I I
I " l1 X0 30 20 UpperI I
I I
I I
I p I
I I
'I I
I I4 0
USEPA Upper PMSD Bound (90a percentile < 47.0%)
I I
i I
I I
g I
I I
I I
I I
I I
d*
1 ee 10 n
Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD t 2 Standard Deviations)
- .aptis gnunisd fmm5 zmcted axcel sprmadswLt agce uesw 1r0 o1ume17 Page 107 of 117
H S"Precision of Endpoint Measurements Ceriodaphnia dubla E... T
.S Chronic Reference Toxicant Data Environmental Testing Solutions, Inc.
Test Control Control Mean a
Test date C
number Survival Reproduction for Control Mean
(%)
(offspring/female)
Reproduction (offsprlng/female)
CV Cr.
MSD PMSD Cr for Control N5)
Reproduction CV (%)
(%)
for PMSD (%)
1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 12-04-12 01-08-13 02-05-13 03-05-13 04-02-13 05-07-13 05-07-13 06-04-13 07-09-13 08-06-13 08-06-13 09-10-13 10-08-13 11-05-13 12-03-13 01-07-14 02-04-14 03-04-14 04-08-14 05-06-14 100 100 100 100 100 100 100 100 100 100 100 IlOO 100 100 100 100 100 100 100 100 1oo 31.9 30.8 32.1 31.4 28.7 30A 32.2 29.7 30.7 31.0 33.9 29.2 31.9 31.2 32.3 30.6 30.7 313.
32.4 31.3 31.4 31.6 31.6 31.0 30.9 31.1 30.9 30.9 30.9 31.2 31.0 31.1 31.1 31.2 31.1 31.1 31.1 31.2 31.2 5.2 5.5 6.3 4.8 5.9 SA 6.2 5.0 6.9 4.6 5.5 5.5 5.4 2.9 4A 6.0 3.5 3.4 6.7 5.2 5.3 5.7
.5.4 5.5 6.0 6.0 5.9 6.0 5.9 5.8 5.8 5.8 5.6 5.5 5.5 5.4 5.3 5.4 5.4 1.9 2.0 2.0 1.7 1.9 2.2 2.4 1.7 1.6 1.7 2.1 1.6 1.5 1.8 1.7 1.9 19 1.8 2.1 1.7 6.0 6.5 6.1 5.6 6.8 7.3 7.3 5.7 5.3 5.3; 6.1 5A 4.6 5.6 5.2 6.1 6.2 5.9 6.5 5.5 6.3 6.2 6.0 6.2 6.4 6.5 6.4 6.3 6.2 6.2 6.1 6.0 6.0 5.9 5.9 5.9 5.9 6.0 5.9 Note:
CV Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (le percentile) = 8.9%.
Upper CV bound determined by USEPA (90P percentile) = 42%
MSD Minimum Significant Difference PMSD= Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10 th percentile) = 13%.
Upper PMSD bound determined by USEPA (9e* percentile) = 47%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimifiation Program. EPA-833-R.00-00S. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronicand Acute Whole Effluent ToxicityTest Methods, Volumes I and 2-Appendix. EPA-821-B-01-0C4 and EPA-821-9-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICR.050614.xlsx Entered by: J. Sumner Pagviewed by.o Page 108 of 117
I i
I i
I I
0ETS Page Iof 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceiodaphnia duba CdNaCICR#:
-16A Dilution Preparatdon infornaon:
Comrnais:.
NaCI Stock DISS mmbcr 193
ý Stock prperaion:
100 g NaCI/L:
Di..olvee zOS aC in 500mL WlI-Owvc.
Dilution IV_
(mg/L) 60 goo 1000 12U0 1400 Stock volume (L)
.9 12 1i5 181 21 Diluent volume (mL) 1491 1483 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Tet ogM m surce Wrmnado.
Tet ifomadow Oaganism ie=
.<c24-hbo old Randomizing temnpyh color:
Dae and times orgms werbom s-e-'
01.4
-it OW huebatornumim and shelf between:
loc2on:
Cultumbeard:
"-t Replicate number. L 2 1 1 4 1 5 1 6 1 7 1 8 1 9 1 30o YNWbat*:
1 Trnsfr vsselinfnanow-H -- 1.%VS..
ýtmýSelsnasgnjm batckh Daily renewal Information:
Day Date Test Initiation and feeding, MHSW Analyst renewal and feeding, or bptch used termination time
_____m-,
otto jL 0
, f os.06-
-o 9.. ICi.
-1 o.
5 fos-a-.l4 I____*:__'__<
o-aA 6
cc.___
o1%c4N IL.
Contro WsFVInnal Accepteace afei W---,O
~
endpoings S% of Male Aults:
1 25"%
Zf CA.....
% Aduts having 3Broods:
(OQT.
k
.30%
NOEC AO
% Motaty:
01
- " 20%0/
LOECW Mean Ospring/Female:
1-'b k 15.0O *.p'gImale ChV
- g. q
%CV:
I 2.7.
< 40.0 %
IC-m im.-I (i..fl SOP AT14 - Exhibit AT14.1, revision 06-01-11 Page 109 of 117
Page 2 of 6 p
I I
I Species: Ceriodaphnia dub*ia CONTROL CdNaCICR#:
_to_
Survim and Reproduction Data II I
I I
Replicate number D
y o
1 2
3 4
5 6
7 8
9 10 Adult mortalty I
2 Young produced C1 0 _
. n D
. Aduotmortalifty J t
43 Youngprodueed 0_
0_
0L
~
M
~
Adult mortality j-..
4' Young produced 1
2 t L 2 L.JIi 5
Young produced v
'0
%:1 4b a
1 1-j- -
Adult mortality I
7 Young produced I&L
.-L L
i_,
Total young produced
~O 2i 3
Final Arodul Motlt AI-C bote: Adult mortality (L - live, D - dead), SB = split brood (single brood split betwen two days), CO - cay over (ofpdng canred over with adult duing lrnmfr).
Conceiuraten:
I%Mortality' I
(*7.,
Mein 2arin ae:
.* [
600 mgNCIL Survival and Reprodadion Dat Rep-icate number Day 1
2 3
4
.5 6
7 8
9 10 1
Young produced Adult motal I."....
2 Young produced 0
____I Adult mortaity
%- Eck)- rt._
z Youngproduced 1C.
1O I
Z I
0 0
Adultrmortal!
iit[_y i
.4 Young produced 59 r3 AdutmorUW W _
Adultmortality j
1.. _
j S
oung produced~
i__
~~I~
71Young produced
~
)
0 A1T Total young produced.
5IL. '
5IL WA a,
3%
a, IlFial Adult'Mortality t..
t_
Note: Adult mortality (L - live, D = dead), SB - split brood (single brood split between two days), CO - carry over (oftpfng carried over with adult duringafnsfer).
% Mortali:
Mean Offs iemale:
aDaA
% Reduction from Conrol:
SOP AT14 - Exhibit ATI4.1, revision 06-01-11 Page 110 of 117
I Ii II p
I II I
I I
TStu Page 3 of 6 Species: Ceaiedvhpn dubia 800 mNaCI/L CdNaCICR#:
116 \\
Swrvib and Reproduction Data lReplicate number Day 1
2 3
4 o
1 6 7
8 9
10 1
Young produceda 0
0 Adult mortal
_0 L
Y
_ugpredueed 0I T
C) 0 e-
=\\
4 Young produced O
C
-,_Adult mortality.-
Adult mortalit
\\_
I k
-N-
%.- I \\-
6 Yon produced LiC.
Adul Mmo it..y.
6%1_eddatidn fro_
- 1o po7 7 Young produced
%% vs-vs I
- n
%atm Total young produced
?I bb
-& ;B 14 1 Ti 1\\~
Plnd Adult Mortality X'ot: Adult
,Mktly (L"- livre D - dead SB pit broad (snge brood split between two daysl CO carry over (o
.tring cmried over with adult during
_ren&.r).
% Mo 1.it Mean Of rpring/ffemale:
&.I
%Reduction from Control:
z6 7 10004)
NaCI/L SurvisaI and Reproducton Data Da 1
2 415
]6 Ti7l 8
9 1 0 Adult mortalit L_
L_____
=
Young produced
~__
I Adultinora it Yon L.r 0 ~
C).
3 Noung produced r
IAdultmortflity
~L.
~
1Yougproduced I t) 17-I 5
-W IW-W to I
Adult mortaft C_
C-C.
C~ t-I.
61 Young produked C0 F,~
~
~
~~r i
Adult mortulity T
L.C-7jYoung producedI I
t%-
%b I r com young pro"UCCu 1 2h 1 7-40 141 1 1A I 19 1 "Zi A 0 1 -4 1 -L e h, - " j k -
\\._ I. -
I_ %- I -C -
1 11..
Note. Adult mortality (L - live, D - dead), SB - split brood (single brood split between two days). CO = carry over (oihprln carried over with adult during transfer).
Concentradon:
1
%Mo 0.
Imean OffsprWg/emale:
I
% Reduction from Control:
I SOP AT14 - Exhibit AT14.1, revision 06-01-11 Page 111 of 117
I I
II I
I, II ii
~Iji Ii j
II Ii I
I a:TS Page 4 of 6 Species: Cerfdanpkia dubia 12(0 'n NnlI/T, CdNaCK1 #: jkL.
Survival and.R*roducon Data Replicate number Day
' 1'
.2 3
14 5
6 7
8 9
10
=
_Young pr 0du c ed
_o o
0 0_
Adlto,______.
=
t, k -- L-2 Young produced 0
0 C)C)f 1
~,*.,,
i I.
L.
.J "L "
3 Young proIuced 0- '
ci 0
Adul t mortality j L
L
? j 1._
4 1("m prwdpI*"
AdultWfliY 110.t w
.y.
Ne Adultmorll~livemDd od.-,Oo
-o 6
Young.
i oduct d
J'I r.
Adult xaortality 7
oung produced [
i
~
~
.Q
__ _ ___I Fina Adl Mors 1taftL Note: Adult mortality (L =live, D dead) SB split brood (s ingle brood split betwentwo days). CO) - ory over (o~fpr1ng E C.o,,mce'n&;a" k.
" IA:Morw.T I
7
.. IMew Offs:
I l O.
al!
.- I ed, tnfromConmtrol: I -A'Z Surviva Wad Rerodution D)ata 1400 mg NaCI/L Day 2
3 3
-4 6
7 1
i 9 I
Youn g produced 0
0 0
2 Young produced 0
0*,
-b0Ib t"""~
A Adult, ortal.y o. ".
i t
X-
= Aduit*
ortalIty I
3 1 Young produced-j_
,,,o.Adult
.iortatiyf
= K 5Youngproduced L
I
~
~ ?
6 Young produced Q c>1c T
0 6
0 A
rdult d
CotDit C)
I Total young produced/
l I
Bioal Adult Mortality
~~~-
Note: Adult mortality (L - live, D - dead), SB - split brood (single brood split btween two days), CO - carry over (oflprfing carried over with adult during trnsfe).
Ccocei;ade
% Mortai.I.,
I Man Ofpringem*ue:
2.5
% Reduction from Control: i j
SOP AT14 - Exhibit ATI4.1, revision 06-01-11 (f.I l...
Page 112 of 117
lETS Verification of Ceriodaphnia Reproduction Totals Control R
number Total DAY 1 1 2 1 3 4T 5 6n 1 11 I
I 1
0 0
0 0
0 0
0 0
0 0
2 0
o 0 o
0 o
0 0
0 0
0
- 3.
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
4 6
4 5
5 5
46 5
12 12 10 12 13 10 11 12 12 13 117 6
.0
- 0 0
0 0
0o 0
0 7
151 161516 14 1 61316 150 Total 1
32 30 31 33 30 29 33 3
313 W
NaCi/M Day nubte r TUober 11 2
3 4
5 6
7 18 1 9ota0 1
0
- 0.
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0 10 0
0 o
3 o0 0 0
0 0
0 0
0 0
0 0
4 5
4 6
5" 5
4 5
4 5
4 47 5
12 12 1_
12 10 11 11 13 11 1 121 117 1
6 0
0 0
0 0
0 1 0 0
0 0
7 15 17 13 14 16 13 1 13 116 145 Total 32 33 32 31 31 28 29 30 131 32 30 800 MR NaCI/L Da ROPlieate number Total D
2 3 14 5
61 7 10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 a 0
3 o00 o
o o
o 0
0 0
0 4
4 5
4 4
4 5
5 5
- 5.
4 45' 12 12 12 11 12 12 10 11 11 11 114 7
1416 14 IS 15 15 15 113 15 14 146 T30313230293129 305 100 iNaCVL ak Rev*ste number Total 1
2' 3
4 5
6 7
8 9
10
-1 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
3 4
5 4
4 4
4 3
3 38 S
101012 12 10 to 10 10 11 105 6
0 0
0 0
0 0
0 0
0 0
0 7
13 14 14 12 15 13 15 16 14 I4 140 Toa 7 2
0 29 19 28 129 130 2612 1200m NaCi/L Redileate nunber Day 2 13 4
5 6
7 8-9 10 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0 1'0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
00 0
0 0
0 0
0 4
3 4
2 2
1 3
1 2
4 1
23 S
0 0
0
- 0.
0 0
5 0
0 6
11 6
8 5
9 6
6 3
0 10 5
0 52 7
0 o
0 o
0 9
0 0
5 14 Tota 11 9
1 8
'7 6
1 12 9 a 1
100 1400 NaCIIL Day gfileate number Total 2
3 4
.5 6 1 7
.8 9
10 0 0 o
o o
o o
o o
o 1
,0 2i 0
0 0
0 0'~ 0
- 0.
0 10 0
0 3
0 0
0 0
0 0
0 0
0o 0
0 4
0 0
2 0
1 0
0 0
0 3
6 5
2 1
0 3
0 2
.2 1
3 0
14 6
0 0
0 0
0 0
0 0
0 0
0 7
0 0
I 0
3 0
0 0
0 1
5 Total 2
1 3
3 4
2 2
3 1.4 1 25 File: CdNaCICR._O506l4jxsx Entered by J. SuIpner Reve._d by:.
Page 113 of 117
.ETS 6Environmental Testing SacLulons, Ic Ceriodaphida dubla Chronic Reference Toxicant Test EPA-821-R-}2-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
Test dates:
CdNaC1CR #161 MAy 06-13,2014 Concentration
_Repli._cate number
_Survival Average reproduction CoeCMat of Pate.t reductim from (mg/L NCO) 1 2
3 4
5 6
7 S
9 10
(%)
A
)
(%)
Control 31 32 30 31 33 30 29 33 30 34 l00 31.3 5.2 Not applicable 600 32 33 32 31 31 28 29 30 31 32 100 303 4.9 1.3 IN 30 33 -
30 30 31 32 30 29 31 29 100 3.5 4.2 2.6 1000 27 27 30 29 29 28 29 30 26 28 100 28.3 4.1 9.6 11 9
11 8
7 6
15 12 9
12 1
10
.10.0 27.1 68.1 1400 2
1 3
3 4
2 2
1
.3 4
100 2.5 43.2 92.0 unnet's bSD value-1.716 MSD =
Minimum Significant Diffaence PMSD 5.5 FWMSD =
Percent Minimum Significant Difference PMSD is a measue of test precision. The PMSD is the minimum pecnt diffimnce.bemen the control ad treatmt in can be declared sttcally significant in a whole efuent toxicity test Low PMSD bound detrinined by USEPA (1 percentile) - 13%.
Upper PMSD bound determined by USEPA (90 pearcentile) =47%
Lowe mad upper PMSD bounds were detrmined ftom the 10th and 90th percentile, respectively, of PMSD data Anom EPAs WET Intrmlaboratory Variability Study (USEPA. 2001a; USEPA, 200 1b).
USEPA. 2001a, 2001b. Final Report Inhraboratory Vafiability Study ofEPA Shot-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volunes I and 2-Appendix EPA-821-I-01-004 and EPA-821-B-01-005.
US F.rvinnentul Pmrtection Agency, Cincinati, OhI Ffle: CdNaCICR..050S14.xtsx Table populated from associated Verlflcation of Ceiodaphnw Reproduction Totals" spreadsheet Spreadsheet entered by. J. S'rmnr Reviewed by-.
Page 114 of 117
0ETS SEmI m~ma4Th 1nsq f uu~mhk*
Statistical Analyses Start Date:
5/862014 Test ID CdN*CICR Sample ID REF-Ret Toxicant End Date:
5113=2014 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
NACL-Sodlum chloride Sample Date:
Protocol: FWCHR-EPA-821-R-02-013 TeatSpecies:
CD-Corlodaphlla.dwbis Commandt:
Cona-rnglL 1
2 3
4 5
6 7
8 9
10 D-Contro 31.000 32.000 30.000 31.000 33.000 30.000 29.000 33.000 30.000 34.000 600 32.000 33.000 32000 31.000 31.000 28.000 29.000 30.000 31.000 32.000 800 30.000 33.000 30.000 30.000 31.000 32.000 30.000 29.000 31.000 29.000 1000 27.000 27.000 30.000 29.000 29.000 28.000 29.000 30.000 28.000 28.000 1200 11.000 9.000 11.000 8.000 7.000 6.000 15.000 12.000 9.000 12.000 1400 2.000 1.000 3.000 3.000 4.000 2.000 2.000 1.000 3.000 4.000 TransFor: Unitansfored 1-Taleld Isotonie Conc-mngL Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 31.300 1.0000 31.300 29.000 34.000 5.228 10 31,300 1.0000 800 30.900 0,9872 30.900 28.000 33.000 4.932..10 0.633 2287 1.716 30,900 0.9872 800 30.500 0.9744 30.500 29.000 35.000 4.162 10 1.068 2287 1.716 30.800 0.9744 11000 28.300 0.9042 28.300 26.000 30.000 4.728 10 3.697 2.287 1.716 28.300 0.9042
- 1200 10.000 0.3195 10.000 6.000 15.000 27.080 10 28.379 2.287 1.716 10,000 0.3195
- 1400 2.500 0.0799 2.500 1.000 4.000 43.205 10
.38.372 2.287 1.716 2.500 0.0799 Auxiliary Teote StatIstic Critical Skew Kurt Kolmogorov D Test indfcates normal distribution (p > 0.01) 0.58144 1.035 0.17181 0.66778 Bartlett' Test Indic.aes equal varlances Co = 0.07) 10.1883 15.0883 Hypothsois Test (1-tall. 0.08)
NOEC LOEC ChV TU MSIDu MSDp MRB MOE F7Pwb df Dunnett'lmTest 800
" 1000 894.427.
1.71827 0.05483 1803.03 281667 t.E-45 5.54 Treatments vs D.Control Linear Interpolation (200 Resamplas)
Point malL 80 06% CL Skew IC05 869.545 59.6189 724.943 940.909 -2.0963 IC10 1001.42 25.3546 924.747 1010.48 -2.3294 IC1S 1018.52 5.19633 1007.39 1027.18 -0.3039 1.0.
IC20 1035.63 5,00487 1024.72 1044.84 -0.2890 IC25 1052.73 4.97018 1042.77 1062.29
-0.1919 IC40 1104.04 8.7711 1093.63 1116.5 0.1471 0.81 IC50 1138.25 6.88117 1127.54 1153.32 0.2...922 0.7 0.5:
0.4 0.3 0.2 0.1 0.o0 0
N00 1000 1500 Dose m g/L Dose-Response Plot 1-tall, 0.06 level of signtficanco II ts.rm.Ihafl NoIa: CdNsCICR_0SGI14jdox btnmdbr 3, Sunme Reviewed by:r1/2&
Page 115 of 117
I.
6:ETS Page 5 of 6 Species: Ceriodaphnia dubia Daily Chemktv:
CdNaCICR #:
Vto n
eaq Day*
o i
Q:*..gf*mrt idetified fo 0% týpeimedpitV.0; and 2gtq!k. aneensonly.)
I m I
I I
I Cncentration.
CONTROL IDO 0ig9L).
- 1Harwinems (m CA00 3/L)
[DO (mg/VL 600 aug NaCI/L IcomwdviytyI (JU1111o/0111j PH (S.U.)
DO5 (mg/L)
Conductlwly (jamhos/m) 800 mg NaCViL
- Temperature (Q pH (S.U.)
a.OZ.
DO (mi)
-4,A 1000 mg NaCI/L Conductvity
_________*Temperature
(%')
d PH (S.U.)
ýot DO (ig/L) 1400 mg NaCIIL conduetvity V46 0
- Temperature (0Q)
IS-2.-
PH (oSnUt)
S-y*1 DO (mg/L) 1 1400.g INaCIUL ConductivIty Temperature (C STOCK Conductiviy II
- Tempea e plfomned at the time of test initiation, renewal or temination by tie analyst identfled in the Daily Renewal InformatiOn table located on Page 1. Alkalinity and hardness performed by the analyst ideAtified on the beach sheet specific for each analysis and transcribed to this bench sheet by:
SOP ATI4 - Exhib*ft ATI4.1, revision 06-01-11 fr Page 116 of 117
I, I.
."ETS Page 6 of 6 Species: Ceriodaohnia dubia CdNaClCR N:
%XPA 1
(An~vt de~f~dfn w
d,,~n~pDay cnnttvmsielet m,
I IIn t ietidfracd-r-f---
-M D.O.
ad---fffvesue I
3 I
4 I
5 I
6 AnaIst Concentration
[israuseter CONTROL PH (S.U.)
DO (ingL)
Conducftivty (AsMheCM) flardness
- Temperature 4~E U -
~
I PH *.U.)
q.o& I -+,go 1 11 --m7m I D v m V U
600 mg NaCI/L DO (mg/L)
Conductivty
- Temperature I~14.
-;~ ~9 H
Q I
11IIJA.1 1 U ~
U 4
U -0 4U.
pH (S.0.)
qAI 11 -+-3D 1 11 R14 I 14,.Qt(
I 800 mg NaCI/L DO (mg/L)
Conductivity
- Temnperature (00~
La I 'T I
PH (**..)
I
.A DO (mg4,)
VZ 1000 mg NazCIL ConduetiVIqy (tuohost/.)_
- Temperature "s-lID 2v*
0 At '
PH (S.U-)
0HDO (mg/L) 1200 mng NaCI/L CondwAyby~
DO (mg/.)
"Temperature
- t.
t
".j M-Iinitial Wllt 01 Iitil Ulnel --
B I=
~
"Temper M
atu rasnr~
d at the tme often intiation, renewal or tervnbuiati by the apalylt-Idatfe hdlbnmtion table loatd on Pap,.. Allcalinty and hardnmss perfrmemd by the aalynt !idenfiteF hb analysis and transcribed to this benoh shee by:
(f t
SOP AT 14.*x i AT14. 1, rviion 06-01-11 Page 117 of 117