ML13318A891
| ML13318A891 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 11/12/2013 |
| From: | John Carlin Tennessee Valley Authority |
| To: | Morgan C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML13318A891 (114) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 November 12, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2013 Enclosed is the October 2013 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. Also included is the revised outfall 101 T data with results from September 2013 toxicity for which no adverse effects were. observed. If you have any questions or need additional information, please contact Spencer Whittier by email at sdwhittier@tva.gov or by phone at (423) 843-6714.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. /
am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, on ipe President Sequoyah Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
S58 131112 800 - NPDES CORRESPONDENCE November 12, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Resources William R. Snodgrass Tennessee Tower 312 Rosa L. Parks Avenue, 11 th Floor Nashville, Tennessee 37243
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR OCTOBER 2013 Enclosed is the October 2013 Discharge Monitoring Report 'for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. Also included is the revised outfall 101 T data with results from September-2013 toxicity for which no adverse effects were observed. If you have any questions or need additional information, please contact Spencer Whittier by email at sdwhittier@tva.gov or by phone at (423) 843-6714.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, President h Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 S.D. Booker, MOB 1F-WBN B. E. Brickhouse, BR 4A-C J. T. Carlin, OPS 4A-SQN L.S. Cohen, BR 4A-C J. A. Cross, POB 2A-SQN T.R. Markum, BR 4A-C U.S. Nuclear Regulatory Commission Attn: Document Control Desk Washington, DC 20555 M. McBrearty, OPS 4A-SQN D. B. Nida, BR 4A-C A. A. Ray, WT 11A-K G. R. Signer, WT 6A-K P.R. Simmons, POB 2B-SQN
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if D0erent)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
.-.TEROFFICE OPS-5N-SQNS)
SODDY - DAISY, IN 37384 Faci*W_
TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TNO02450
-101 G
PERMIT NUMBER DISCHARGE NUMBER FNrTRING OPERO YEAR*
MO
- A
{
YA
?6O A
From 13 1( 0 01 1 To, 13 110 1 3ý1 MAJOR Form Approved.
(SUBR 01)
OMB No, 2040-0004 F - FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 38.3 04 31/ 31 RCORDR CENTIGRADE MEASUREMENT 00010 1
0 PERMIT Req. Mon.
DEG. C.
CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS.
TEMPERATURE, WATER DEG.
SAMPLE 25.8 04 0
31 / 31 MODELD CENTIGRADE MEASUREMENT 00010 Z
0 PERMIT 305 DEG. C.
CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAIL MX NUOUS TEMP. DIFF. BETWEEN SAMP. &
SAMPLE Y
2 04 0
31131 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1
S PERMIT 3
DEG. C.
CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1713 03*
0 31/31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 PERMIT Req. Mon.
MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE 0.022 0.049 19 0
23/31 GRAB MEASUREMENT 50060 1
0 PERMIT 0.1 0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE oF SAMPLE 0
0 31/31 CALCTD CHANGE MEASUREMENT 62 82234 1
0 PERMIT 2
DEG CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX CIHR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE lirection or supervision in accordance with a system designed to assure that qualified person John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or(
persons who manage the system, or those persons directly responsible for gathering the I
resident 423 843-7001 13 11 information, the information submitted is, to the best of my knowledge and belief, true, accurate, 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, IG U E OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
R OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injections occurred: 1. Floguard MS6236 (max. calc. conc. was 0.05mg/L--limit 0.2mglL)
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include Facility NamelLocation if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 S_
.ITEROFFICE OPS-5N-SQN.
SODDY - DAISY. TN 37384 Facjily_ TVA - SEQUOYAK NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
E TN0026450 t,
z10 PERMIT NUMBER MDISCHRRG MdONITORING PERIOD YEARMO DAY 1 YEAR MO IDAY From 13 i 10 01 To 1 13 10 31 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE Z NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE Monitoring 23 CERIODAPHNIA MEASUREMENT Not Required TRP3B 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE Monitoring 23 PIMEPHALES MEASUREMENT Not Required TRP6C 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Lirection or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin prmperly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the ent information, the information submitted Is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNA"LUR/OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was not sampled in October 2013.
EPA Form 3320-1 (REV 3199)
Previous editfons may be used Page 1 of 1
PERMIT-EE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 S-IJNTEROCFFIE OPS-5N-SQN).
SODDY - DAISYTN 37384 F.aii
._WA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
Form Approved.
OMB No. 2040-0004 TN0026450 11 103 G F-FINAL PERMIT NUMBER NDISCHARGENUMBER LOW VOL. WASTE TREATMENT POND F
MONITQRING PERIEFFLUENT I YEARI MO PDAY_
LYEARi -MO
-1 DMY From[ 13 ]10 101 01 13 101 31 ATTN: Spencer Whittier NO DISCHARGE
[
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7
8 12 0
17/31 GRAB MEASUREMENT 00400 1
0 PERMIT 6
9 SU THREE/
GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 14 16 19 0
2 /31 GRAB MEASUREMENT 00530 1
0 PERMIT 30 100 MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE
<5
<5 19 0
2 /31 GRAB MEASUREMENT 00556 1
0 PERMIT 15 20 MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.222 1.425 03 0
31/31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 PERMIT Req. Mon.
Req. Mon MGD SEE RCORDR EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly.gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the nt information, the information submitted is,to the best of my knowledge and belief, true, accurate, 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNA OF RINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3t99)
Previous editions may be used Page 1 of 1
PERMITTEE NAMEIADDRESS (Include Facility Name/Location ff Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (I-LNTEROFMCE OPS-5N-SQN)
SODDY - DAISY. TN3738*
Faajj:t TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Spencer Whittier NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 1
110 G I F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved.
OMB No. 2040-0004 MONITORING PERIOD I EFFLUENT YEAR I MO I DAY I YEAR1 MO I D6y From A13 10 01 Toj41jI 1
NO DISCHARGE [i NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE I
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 04 CENTIGRADE MEASUREMENT 00010 1
0 PERMIT REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS TEMPERATURE, WATER DEG.
SAMPLE 04 CENTIGRADE MEASUREMENT 00010 Z
0 PERMIT 30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. &
SAMPLE
- 04 UPSTRM DEG.C MEASUREMENT 00016 1
0 PERMIT 5
DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 50050 1
0 PERMIT Req. Mon.
MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous CHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 50060 1
0 PERMIT 0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE 04 CHANGE MEASUREMENT 04 82234 1
0 PERMIT 2
DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UoUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE lirection or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the esident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIG OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFI R OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMIT-EE NAMEIADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
- INTEROFICE OPS-5N-SQN)-
SODDY - DAISY. TIN 37384 Faclity TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 1
T F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIOD
-= EFFLUENT Form Approved.
OMB No. 2040.0004 I YEARI MOD I
YEAR MO DAY From[ 13 1010611 To I3 I0 3
ATTN: Spencer Whittier NO DISCHARGE
[
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT 23 TRP3B 1
0 0
PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE 23 PIMEPHALES MEASUREMENT TRP6C 1
0 0
PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the re ent information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false Information, SIG E
PRINCIPAL EXECUTIVE TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.
ROF C
OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) ftwous ediftons maybe used Page I of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 IjNTEROFFICE OPS-5N-SQN4)
SODDY - DAISY, TN 37384 Faacily TVA - SEQUOYAH NUCLEAR PLANT Loýation HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN 6
118 G I F-FINAL PERMIT NUMBER.
I I DISCHARGE NUMBER WASTEWATER & STORM WATER MONITORNG
__EFFLUENT Form Approved.
OMB No. 2040-0004 ATTN: Spencer Whittier Frm I
ToY I
I0 I DAY I
- " NO DISCHARGE
[i NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT 19 00300 1
0 PERMIT 2
MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT MINIMUM
- WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1
0 PERMIT 100 MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK' SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 25 00545 1
0 PERMIT MLIL ONCE/
GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1
0 PERMIT Req. Mon.
Req. Mon.
MGD ONCE/
ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the nt information, the information submitted is, to the best of my knowledge and belief, true, accurate, Ice ent 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIG"A@
F PRINCIPAL EXECUTIVE I
i including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED ICODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
-(INTEROFFICE EP:-5.__N-._.SSQ) -
SODDY - DAISY, N. 37384 Faci_
T S.EQUOYAH NUCLEAR PLANT Locaion HAMILTON COUNTY ATTN: Spencer Whittier NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) 0MB No. 2040-0004 (SUBR 101 T F - FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 I
MONITORING PERIOD I
EFFLUENT YEAR MO NAOY MO DYDISCHARGE From 13 09 01 I To] 13 i
3o0 NO d
s b
m t
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 1 / 180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM
%ANNUAL 1C25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 11 08 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNTk PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
O CFIC AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity sampling occurred September 15-20. The test results were received and are included in this corrected September 101T DMR.
EPA Form 3320-1 (REV 31U5)
Previous editions may be used Page 1 of 1 EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
.1 TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: October 10, 2013
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 7. 1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: September 15 - 20, 2013
- 10. Average Flow on Days Sampled (MGD): 1698.89, 1716.82, 1721.29
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: September 17 - 24, 2013
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC 25 = 43.2%
- 17. Test Results: Outfall 101: Pimephales promelas: IC 25 > 100%
Ceriodaphnia dubia: IC_25 > 100%
UV treated Outfall 101: Pimephales promelas: ICj.> 100%
Page 1 of 105
- 18. Facility
Contact:
Spencer D. Whittier Phone #: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodarass Phone #: (256) 386-2787
- 22. Notes: Outfall 101 samples collected September 15 - 20, 2013, showed no toxic
-effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Growth was significantly reduced in minnows exposed to intake samples.
Fathead minnowswere also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 2 of 105
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY)
(MM-DD-YY)
Arrival Initial (MM-DD-YY)
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 101 09-15-13 0645to 09-16-13 1430 1.1, 1.6t
<0.10 09-17-13 1034 09-16-13 0545 09-18-13 0935 09-15-13 0700to 09-17-13 1034 Intake 09-16-13 0600 09-16-13 1430 2.1
<0.10 09-16-13 0600 09-18-13 0935 09-17-13 0645 to 09-18-13 1335 2.3,1.01
<0.10 09-19-13 0945 101 09-18-13 0545 09-20-13 0947 09-17-13 0700 to 09-19-13 0945 Intake 09-18-13 0600 09-18-13 1335 0.9
<0.10 09-20-13 0947 09-18-13 0600 09-20-13 0947 09-19-13 0740 to 09-21-13 0950 101 09-20-13 0640 09-20-13 1320 1.2, 1.0t
<0.10 09-22-13 1012 09-0-3 04009-23-13 0948 09-19-13 0755 to 09-21-13 0950 Intake 09-19-13 0755 09-20-13 1320 0.9
<0.10 09-22-13 1012 09-20-13 0655 09-23-13 0948
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- L.0TC) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart ITV Sterilizer (manuifactuired hv Emnernr Anouatiec_
Inc.) for 2 minutes.
Page 3 of 105
Test Organisms:
Pimephales promelas Aquatox, Inc.
18.38 - 18.57 hours6.597222e-4 days <br />0.0158 hours <br />9.424603e-5 weeks <br />2.16885e-5 months <br /> old Ceriodaphnia dubia In-house Cultures
< 24-hours old
- 1. Source:
- 2. Age:
Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101 10 I
09-17-13 1034ET 09-17-13 1023 ET 09-24-13 0937 ET 09-24-13 0953 ET 09-17-13 0940 ET 09-24-13 0847 ET Mean = 24.90C (24.7 - 25.3 0C)
- 8. Test Temperature: Outfall 101:
Mean = 24.8°C (24.3 - 25.1 °C)
Test Temperature: UV-Treated Outfall 10 1:
(
dean = 24.8°C 24.2 - 25.20C)
- 9. Physical / Chemic Measurements:
al Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
Statistics were performed according to methods prescribed bj EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
- 10. Statistics:
Page 4 of 105
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted September 17 - 24, 2013 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - day s)
(% Effluent) 1 I
2 3
[
4 J
5 6
7 Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 i00 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 95 95 95 93 93 Test Solutions
.Mean Dry Weight (mg)
TestSoutions(replicate number)
(%Effluent) 1 2
3 4
Mean Control 0.576 0.701 0.591 0.633 0.625 10.8%
0.675 0.623 0.684 0.618 0.650 21.6%
0.564 0.592 0.619 0.626 0.600 43.2%
0.550 0.542 0.648 0.568 0.577 86.4%
0.537 0.535 0.630 0.622 0.581 100.0%
0.539 0.633 0.574 0.607 0.588 Intake 0.487 0.467 0.616 0.565 0.534 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc
- 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5o: TUc = 100/IC 25 Page 5 of 105
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted September 17 - 24, 2013 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions
(% Effluent)
Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Test Soluetis Data (replicate number)
(%Effluent) 1 2 13 1415 61718 9
10 Mean Control 28 30 29 28 28 35 27 28 30 28 29.1 10.8%
32 29 30 28 29 32 31 28 28 29 29.6 21.6%
29 32 29 33 30 32 32 31 29 31 30.8 43.2%
34 30 30 32 32 34 29 34 31 33 31.9 86.4%
31 31 30 33 36 35 34 29 34 32 32.5 100.0%
33 36 32 35 35 33 36 31 33 32 33.6 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC 25 Page 6 of 105
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted September 17 - 24, 2013 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 1
1 112 [3~
4j 5 6
(% Effluent) 1I2 3I4_
6 7
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Test Soluetis Data (replicate number)
(%Effluent) 1 2
3 4 15 6 17 8 19 110 Mean Control 28 28 28 30 29 30 30 29 29 30 29.1 Intake 35 31 37
- 31.
33 30 34 33 33 30 32.7 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NAA
- TUa = 100/LC5 o: TUc = 100/IC25 Page 7 of 105
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas (Genus species)
Chronic/ 7-day Toxicity Test.
(Type / Duration)
Conducted September 17 - 24, 2013 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1
[
2 3
4 5
[
6 7
Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg)
(%_Effluent(replicate number)
(%1Effluent) 2 3
4 Mean Control 0.580 0.607 0.517 0.562 0.567 10.8%
0.596 0.614 0.629 0.562 0.600 21.6%
0.583 0.577 0.628 0.615 0.601 43.2%
0.599 0.566 0.621 0.584 0.593 86.4%
0.538 0.590 0.529 0.674 0.583 100.0%
0.570 0.598 0.647 0.607 0.606 Intake 0.618 0.611 0.643 0.567 0.610 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100!LC5 0: TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species I
Date Time Duration Toxicant Results (IC2 5)
Pimephalespronielas September 17-24, 2013 1045 7 days KC1 0.71 g/L Ceriodaphnia dubia September 10 - 17; 2013 1010 7 days NaCl 1.06 g/L Page 8 of 105
(a (D
Cz 0
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pirnephalesprornelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 perfbnned September 17-24, 2013.
Test Sample ID Temperature (C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO3) (mg/L CaCO3)
Chlorine (mg/L)
Control 24.8 24.6 7.9 7.4 7.96 7.64 313 61 91 24.7 -
24.9 24.5 - 24.7 7.7 8.1 6.8 -
8.1 7.85 -
8.07 7.51 -
7.73 306 -
323 60 61 90 92 10.8%
24.8 24.7 7.9 7.4 7.89 7.60 291 24.7 - 25.0 24.4 - 24.9 7.8 8.1 6.8 -
8.2 7.77 -
8.04 7.40 -
7.71 285 -
296 2.%
24.9 24.7 7.9 7.3 7.90 7.58 276 21.6%
24.8 - 25.0 24.4 - 24.9 7.8 -
8.1 6.8 -
8.2 7.76 -
8.04 7.40 -
7.69 270 -
278 Q-24.9 24.6 8.0 7.3 7.88 7.58 243 43.2%/
4 24.8 - 25.0 24.5 - 24.8 7.8 -
8.2 6.8 -
8.2 7.68 -
8.04 7.40 -
7.69 239 -
247 8.%
24.9 24.6 8.0 7.3 7.84 7.57 179 86.4%
24.8 -
25.0 24.3 - 24.8 7.8 -
8.2 6.8 -
8.2 7.56 -
8.00 7.40 -
7.65 175 -
184 100.0%
25.0 24.7 8.0 7.3 7.80 7.55 156 60 66
< O. 10 24.9 -
25.0 24.5 -
24.8 7.9 -
8.2 6.8 -
8.2 7.50 -
7.99 7.38 -
7.65 153 -
157 60 61 64 68
<0.10-
<0.10 Intake 25.0 24.6 8.0 7.3 7.77 7.60 155 61 64
< 0.10 24.9 - 25.1 24.4 -
24.7 7.9 -
8.2 6.8 -
8.1 7.45 -
7.93 7.37 -
7.69 151 -
158 60 61 60 70
<0.10-
<0.10 Control 24.8 24.9 7.9 7.8 7.96 7.86 313 61 91 24.7 - 24.9 24.8 -
25.1 7.7 -
8.1 7.7 -
8.1 7.85 -
8.07 7.80 -
7.91 306 -
323 60 61 90 92 10.8%
24.9 25.0 7.9 7.8 7.89 7.87 291 24.7 - 25.0 24.7 -
25.3 7.8 -
8.1 7.7 -
8.1 7.77 -
8.04 7.81 -
7.93 285 -
296
- 1.6%
24.9 25.0 7.9 7.9 7.90 7.88 276 24.8 - 25.0 24.9 -. 25.0 7.8 -
8.1 7.7 -
8.2 7.76-8.04 7.80 -
7.94 270 -
278 42 24.9 24.9 8.0 7.9
- 7.88 7.88 243
- 4.
24.8 - 25.0 24.7 -
25.1 7.8 -
8.2 7.7 -
8.2 7.68 -
8.04 7.80 -
7.93 239 -
247 25.0 25.0 8.0 8.0 7.84 7.86 179 8
24.8 - 25.0 24.7 -
25.1 7.8 -
8.2 7.8 -
8.3 7.56 -
8.00 7.79 -
7.92 175 -
184 100.0%
25.0 24.9 8.0 8.0 7.80 7.86 156 60 66
<0.10 25.0 - 25.1 24.7 -
25.3 7.9 -
8.2 7.8 -
8.3 7.50 -
7.99 7.79 -
7.92 153 -
157 60 61 64 68
<0.10-
<0.10 Intake 25.0 24.9 8.0 8.0 7.77 7.86 155 61 64
< 0.10 24.9 - 25.1 24.8 -
25.0 7.9 -
8.2 7.8 -
8.3 7.45 -
7.93 7.77 -
7.94 151. -
158 60 61 60 70
<0.10-
<0.10 Overall temperature (C)
Pimnephales promelas Ceriodaphnia dubia Average 24.8 24.9 Minimum 24.3 24.7 Maximum 25.1 25.3
(D 0
PHYSICAL/CHEMICAL SUMM ARY Water Chemistry Mean Values and Ranges for Pimephales proinelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 10 1 performed September 17-24, 2013.
Test Sample ID Temperature (°C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness
- Total Residual Initial Final Initial Final Initial Final (pmhos/cm)
(mg/L CaCO3 ) (mg/L CaCO3) Chlorine (mg/L)
Control 24.8 24.7 8.0 7.3 7.92 7.62 306 60 88 24.8 - 24.9 24.5 -
25.0 7.8 8.3 6.7 -
8.2 7.81 -
8.01 7.46 -
7.72 303 -
311 60 61 84 90 108%
24.9 24.6 8.0 7.4 7.92 7.62 292 24.9 - 25.0 24.3 -
24.9 7.8 -
8.3 6.8 -
8.2 7.80 -
8.00 7.41 - 7.71 283 -
297 21.6%
25.0 24.6 8.0 7.3 7.91 7.63 277 24.9 - 25.1 24.3 - 24.9 7.8 8.3 6.6 -
8.1 7.77 -
8.01 7.42 -
7.71 273 -
280 C-25.0 24.6 8.0 7.3 7.90 7.60 246 443.2%
ts 24.9 - 25.1 24.2 -
24.8 7.9 -
8.3 6.6 -
8.1 7.72 -
8.00 7.41 -
7.71 242 -
248 4
25.0 24.7 8.1 7.3 7.86 7.60 180 86.4%
25.0 - 25.1 24.5 - 24.9 7.9 -
8.4 6.5 -
8.1 7.63 -
7.97 7.43 - 7.71 178 -
183 100.0%
25.1 24.7 8.1 7.3 7.83 7.56 157 61 67
< 0.10 25.0 -
25.2 24.4 - 24.9 7.9 -
8.4 6.6 -
8.1 7.55 -
7.97 7.37 - 7.67 153 -
160 60 61 66 68
<0.10 -
<0.10 Intake 25.0 24.7 8.1 7.3 7.80 7.62 155 61 66
<0.10 25.0 -
25.1 24.4 - 24.9 8.0 -
8.4 6.6 -
8.1 7.54 -
7.95 7.43 - 7.75 150 -
157 60 62 64 70
<0.10 -
<0.10
- Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
Overall temperature (°C)
Average Minimum Maximum Pimnephales promelas 24.8 24.2 25.2
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected September 15 - 20, 2013, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >
100 percent. Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period. Growth was significantly reduced in minnows exposed to intake samples.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 11 of 105
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821 -R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 105
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B-2000.
- 5. The pH was measured by SM 4500-H+ B-2000.
- 6. Conductance was measured.by SM 2510 B-1997.
- 7. Alkalinity was measured by SM 2320 B-1997.
- 8. Total hardness was measured by SM 2340 C-1997.
- 9. Total residual chlorine was measured by ORION 97-70-1977.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in thisreport and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KCI or NaCI.
- 2.
Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page. 13 of 105
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 22 nd Edition, 2012.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 105
Sequoyah Nuclear Plant Biomonitoring September 17 - 24, 2013 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 I-I 09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998
< 0.001 0.044 0.015 09/13/1998
< 0.001 0.044 0.015 09/14/1998 0.008 0.044 0.015 1
02/22/1999
< 0.001 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999
< 0.001 02/28/1999
< 0.001 08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070 0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.006 08/24/1999 0.016 0.067 0.023 0.020 I
Page 16 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000
< 0.0057 0.055 0.019 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000 0.0088 0.055 0.019 07/30/2000 0.0076 0.055 0.019 07/31/2000 0.0152 0.055 0.019 0.006 08/01/2000
< 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.005 12/13/2000 0.0120 0.025 0.020 12/14/2000 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 0.0036 0.025 0.020 12/17/2000
< 0.0036 0.025 0.020 1
08/26/2001 0.017 0.06 0.021 0.006 08/27/2001
<0.0096 0.06 0.021 0.005 0.021 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 0.020 0.005 0.021 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001
<0.005 0.059 0.020 I
11/25/2001
<0.0044 11/26/2001
<0.01 19 0.024 0.02 0.005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001
< 0.0043 0.024 0.02 1
12/09/2001
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001
<0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 Page 17 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 0.0079 0.023 0.02 0.006 01/03/2002 0.0042 0.023 0.014 01/04/2002 0.0124 0.024 0.014 0.009 01/05/2002
< 0.0042 01/06/2002 0.0042 01/07/2002
< 0.0089 0.024 0.014 0.006 1
02/24/2002 0.004 02/25/2002 0.004 0.023 0.023 02/26/2002 0.0143 0.023 0.023 0.007 02/27/2002 0.0041 0.023 0.023 02/28/2002 0.0041 0.024 0.008 03/01/2002
< 0.0041 0.024 0.008 05/05/2002 05/06/2002 0.058 0.02 0.014 05/07/2002 0.058 0.02 0.015 05/08/2002 0.056 0.019 05/09/2002 0.057 0.02 0.014 05/10/2002 0.056 0.019 I
08/04/2002
<0.0058 08/05/2002
<0.0058 0.053 0.018 0.025 08/06/2002 0.0092 0.053 0.018 08/07/2002
<0.0107 0.055 0.019 0.007 08/08/2002
<0.0061 0.055 0.019 08/09/20021 0.0152 0.054 0.018 0.008 1
10/06/2002
<0.00497 10/07/2002 0.0153 0.054 0.018 0.009 10/08/2002
<0.0092 0.054 0.018 0.007 10/09/2002 0.0124 0.053 0.018 0.009 10/10/2002 0.0134 0.054 0.018 0.009 10/11/2002
<0.0042 0.054 0.018 01/12/2003
<0.0035 01/13/2003
<0.006 0.025 0.019 0.009 01/14/2003
<0.01 18 0.026 0.020 01/15/2003
<0.0063 0.026 0.020 0.009 01/16/2003
<0.0034 0.026 0.020 01/17/2003
<0.0034 0.026 0.009 04/06/2003
<0.0073 04/07/2003
<0.0189 0.021 04/08/2003
<0.0117 0.021 04/09/2003
<0.0139 0.021 0.016 04/10/2003
<0.0113 0.021 0.018 04/11/2003 1 <0.0073 1
0.022 I
I Page 18 of 105
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 0.0045 06/16/2003 0.0037 0.057 0.020 0.022 06/17/2003 0.0048 0.041 0.014 0.024 06/18/2003 0.0048 0.041 0.014 0.024 06/19/2003 0.0085 0.058 0.020 0.025 06/20/2003
< 0.0048 0.058 0.020 1
0.025 08/03/2003
<0.0050 08/04/2003
<0.0050 0.058 0.020 08/05/2003
<0.0051 0.057 0.020 0.025 08/06/2003
<0.0084 0.057 0.020 0.025 08/07/2003 0.0129 0.057 0.020 0.024 08/08/2003 0.0153 0.057 0.020 0.009 10/05/2003
<0.0043 0.057 0.020 10/06/2003
<0.0043 0.057 0.020 0.025 10/07/2003
<0.0090 0.057 0.020 0.025 10/08/2003
<0.0106 0.057 0.020 0.025 10/09/2003 0.0181 0.026 0.022 0.025 10/10/2003 0.0183 0.026 0.024 0.009 02/01/2004 0.0093 0.027 0.009 02/02/2004
<0.0034 0.026 0.009 02/03/2004
<0.0034 0.026 0.009 02/04/2004 0.0124 0.026 0.009 0.009 02/05/2004
<0.0034 0.026 0.009 02/06/2004 0.0105 0.026 0.009 0.010 05/04/2004
<0.0123 0.026 0.019 0.025 05/05/2004
<0.0144 0.026 0.014 0.009 0.025 05/06/2004
<0.0146 0.037 0.013 0.025 05/07/2004 0.0227 0.058 0.020 0.009 0.025 05/08/2004 0.016 0.060 0.021 05/09/2004
<0.0104 0.058 0.020 07/04/2004 0.0217 0.057 0.019 07/05/2004
<0.0085 0.057 0.020 0.009 07/06/2004
<0.0077 0.058 0.020 0.031 07/07/2004 0.0252 0.056 0.019 0.031 07/08/2004 0.0223 0.057 0.019 0.009 07/09/2004 0.0182 0.057 0.020 0.009 Page 19 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e
TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004
<0.0187 0.000 0.014
- I 11/08/2004
<0.0192 0.047 0.030
-0 1110912004
<0.0233 0.048 0.016 0.041 11/10/2004
<0.0149 0.047 0.016 0.041 11/11/2004.
<0.0149 0.049 0.017 0.043 11/112/20041
<0.02531 0.048 1
0.017 0.042 02/06/2005
<0,0042 0.028 0.010 02/07/2005
<0.0116 0.028 0.010 0.007 02/08/2005
<0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005
<0.0042 0.028 0.010 I
0 02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 06/06/2005 0.0043 0.037 06/07/2005 0.0103 0.037 06/08/2005 0.0295 0.037 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 0.036 07/19/2005 0.0163 0.026 0.009 0.036 07/20/2005 0.0209 0.026 0.009 0.014 0.036 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 0.018 0.014 10/30/2005 0.0068 10/31/2005 0.0112 11/01/2005 0.0104 0.035 11/02/2005 0.0104 0.036 11/03/2005 0.0117 0.036 11/04/2005 0.0165 0.035 11/14/2005 0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 0.0231 11/18/2005 0.0200 11/19/2005 0;0116 Page 20 of 105
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC o
EO/PO Phosphate 11/12/2006 0.0055 11/13/2006 0.0068 0.037 11/14/2006 0.0143 0.037 11/15/2006 0.0068 0.037 11/16/2006 0.0267" 0.037 11/17/2006 0.0222 11/26/2006 0.0188 11/27/2006 0.0138 11/28/2006 0.0120 11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 0.015 05/29/07 0.036 0.015 05/30/07 0.0084 0.017 0.036 0.015 05/31/07 0.0103 0.036 0.015 06/01/07 0.0164 0.017 0.036 0.015 06/02/07 0.0305 0.015 12/02/07 0.0241 12/03/07 0.0128 12/04/07 0.0238 12/05/07 0.0158 12/06/07 0.0162 12/07/07 0.0175 04/13/08 0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.0149 10/26/08 0.0260 10/27/08 0.0151 0.017 10/28/08 0.0172 0.041 10/29/08 0.0154 0.018 0.041 0.030 10/30/08 0.041 0.030 10/31/08 0.0086 0.041 0.030 Page 21 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbronf PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L
-PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mgiL mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 0.0197 0.017 02/09/09 0.0237 0.017 02/10/09 0.0104 0.021 02/11/09 0.0155 0.017 02/12/09 0.0106 0.017 02/13/09 05/10/09 0.0129 05/11/09 0.0415 0.0446 05/12/09 0.0053 0.0396 05/13/09 0.0049 0.0396 05/14/09
<0.0141 0.0397 05/15/09
<0.0160 11/15/09 0.025 11/16/09 0.0152 11/17/09 0.0255 11/18/09 0.0306 11/19/09 0.0204 11/20/09 0.0093 05/09/10 0.0192 05/10/10 0.0055 05/11/10 0.0100 0.039 05/12/10 0.0171 0.039 05/13/10 0.0041 0.039 05/14/10 0.0099 0.039 Page 22 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbrom PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo-mg/L 222 mg/L mg/L
-PFmg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly-DMAD Azole Quat mg/L mg/L Quat mgPL mg/L mg/L Phos-mer EO/PO Quat Phos-Phosphate TRC phate phate 10/31/10 11/01/10 0.0122 11/02/10 0.0112 11/03/10 0.0163 11/04/10 0.0107 11/05/10 0.0132 05/01/2011 05/02/2011 0.04 05/03/2011 0.04 05/04/2011 0.0155 0.04 05/05/2011 0.0179 0.04 05/06/2011 0.0089 11/06/2011 0.0168 11/07/2011 0.0225 11/08/2011 0.0141 11/09/2011 0.0239 11/10/2011 0.0242 11/11/2011 0.0231 05/06/2012 05/07/2012 05/08/2012 0.041 05/09/2012 0.0145 0.041 05/10/2012 0.0298 0.041 05/11/2012 0.0174 08/12/2012
" 0.029 08/13/2012 0.0256 0.028 0.037 0.029 08/14/2012 0.0209 0.037 0.029 08/15/2012 0.0279 0.028 0.029 08/16/2012 0.0076 0.029 08/17/2012 0.0446 I
0.032 05/12/2013 0.0099 05/13/2013 0.064 05/14/2013 0.0091 0.039 0.064 05/15/2013 0.0096 0.039 0.064 05/16/2013 0.0229 0.032 05/17/2013 0.0063 0.032 Page 23 of 105
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - September 20, 2013 Date Sodium Towerbror PCL-PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo-mg/L 222 mg/L mg/L
-PF mgiL mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly-DMAD Azole Quat mg/L mgQL Quat mg/L mg/L mgc L Phos-meo EOePO Quat Phos-Phosphate TRC phate phate 09/15/2013 0.03 09/16/2013 0.0072 0.0379 0.03 09/17/2013 0.0107 0.036 0.0379 0.03 09/18/2013 0.0217 0.036 0.0379 0.03 09/19/2013 0.0172 0.03 09/20/2013 0.0173 0.03
.Page 24 of 105
Sequoyah Nuclear Plant Biomonitoring September 17 - 24, 2013 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 25 of 105
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1-of I I
WUient: TVA fl%
9roject Name: Sequoyah NP Toxicity M
B.O. Number: N/A Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 FedEx UPS Bus Delivered By (Circle One):
Client
&cility Sampled: Sequoyah NP Other (specify): -
, c
- d. l General Comments:
7 k Swkj -FroM /o0-/ SCa, P
j-NPDES Number: TN0026450 Collected By: Dustin Binegar, ia*' s fee-e" l
F 828-350-9368 Container Field Identification/
Grab/Comp.
Collection Date/Time Number &
Flow Rain Event?
Sample Description, Volume (MGD)
(Mark as Appropriate)
Laboratory: Use
~~~~Collected
-bar,
- im,-
.T Yes If"Yes, No Trace ETSLog Arrival Temp.
By Time Appear-.
- i.
- _____.... Yes
- fInches, Number LTa_
- ie ance SQN-101-TOX Comp OS-cr OJs-t 2(2.5gal)
+bq q
'*llrl13 71* Z
.',VI...
S1N-IT-TOX Comp 1
a r/
1(2.5 gal)
A (3"I.'O1 Sample Custody - Fill In From Top Down
" uS1aw*i-WA.i ItAY3 AM V eW-le Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time bt AU. SAf'.
Receivedtt 4
By (Sinatre)
DaeTm I rJ A %.'--2 Instructions: Clients should fill in all areas except those in the "LaboratoryUse" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
0
'ETS 0
Whole Effluent Sample Receipt Log PageLm
- Sample temperature performed using Sample Receiving Thermometer: SN 122164697 Date Time Received Received
- Sample Project Sample S
nst Received Received by from Temp. (C) number number Sample name and description State Comments 09-16-13 1430 J. Sumner TVA Courier 1.1/1.6 9261 130916.01 TVA-Sequoyah Nudear Plant - 101 TN 09-16-13 1430 J. Sumner TVA Courier 2.1
.9261 130916.02 TVA-Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1
of 1
1, lient: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
'Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client 4110.0. Number: N/A Asheville, NC Other (specify): SDoAt, 0,ed,"V-.,"
%acility Sampled: Sequoyah NP 28801 General Comments:
NPDES Number: TN0026450 Phone:
828-350-9364 7
/,91 o
/
Collected By: ý2r L?.At2
, qr r,,.
Fax:
828-350-9368 Field Identification /
Grab/Comp.
Number &
Flow Rain Event?
- piZ, I
Sample Description Volume (MGD)
(Mark as Appropriate)
Laboratory Use Collected..
- VIaA, E**esnd
,a Yes No TraceETS Log Arrival Temp.
By Time Appear-Inches Number ance
- q,*/j q(/g//)
I 1,,
- ~~le i
SQN-101-TOX Comp o
rr e--
o ref r GT-2(2.5gal)
X Z.31f
.K
\\
SQN-INT-TOX Comp
-o oco 12r 1(2.5 gal)
X0 II___________________
Sample Custody - Fill In From Top Down "uobA0 =%6. I u
.*=7.SA Acelueb.
Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time 5&r%ftft.
___,,,,_~
§/
.AL,..--
Instructions: Clients should fill in all areas except those in the "Laboratory User block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
CD 0
IETS w
Whole Effluent Sample Receipt Log Page -VIp
- Sample temperature performed using Sample Receiving Thermometer SN 122164697 Date rime Received Received
- Sample Project Sample Received Received by from Temp. ( 0C) number number Sample name and description State Comments 09-18-13 0948 K. Keenan Fed - Ex 0.6 9268 130918.01 Ahoskie WWTP NC 09-18-13 0948 K. Keenan Fed - Ex 0.9 9269 130918.02 Albemarle - Long Creek WVIWTP NC 09-18-13 0948 K. Keenan Fed - Ex 1.3 9270 130918.03 Carillon Building NC 09-18-13 0948 K. Keenan Fed - Ex 0.9 9271 130918.04 Cleveland WWTP NC 09-18-13 0948 K. Keenan Fed - Ex 1.2 9272 130918.05 Dunn/Blackriver WWTP NC 09-18-13 0948 K. Keenan Fed - Ex 1.7 9273 130918.06 Craven County Wood Energy NC 09-18-13 0948 K. Keenan Fed - Ex 0.9 9274 130918.07 High Point Care Center NC 09-18-13 0948 K. Keenan Fed - Ex 1.6 9275 130918.08 Mayodan WWTP NC 09-18-13 0948 K..Keenan Fed - Ex 1.3 9276 130918.09 Wells Fargo NC 09-18-13 0948 K. Keenan Fed - Ex 1.1 9277 130918.10 Bladenboro WWTP NC 09-18-13 0948 K. Keenan Fed - Ex 3.7 9278 130918.11 ERM - Anschutz Corporation - Outfall 002 MOI 09-18-13 0948 K. Keenan Fed - Ex 3.7 9278 130918.12 ERM -Anschutz Corporation - Up of Out 002 MO 09-18-13 1335 J. Sumner TVA Courier 2.3/1.0 9261 130918.13 TVA - Sequoyah Nuclear Plant - Outfall 101 TN 09-18-13 1335 J. Sumner TVA Courier 0.9 9261 130918.14 1VA-Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page of -I-I!
U
'Vient: TVA a%
(Project Name: Sequoyah NP Toxicity
- R.O. Number: N/A
?ýcility Sampled: Sequoyah N P NPDES Number: TN0026450 Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 FedEx UPS Bus Client Delivered By (Circle One):
Other (specify): Q.*
49.eJjve.1p General Comments:
TooIr e, -f 1 A-,.
/i
/ 0
/-
I I
Relinquished By (Signature):
Date/Time Received By (Signature):
t%3~ etoth (-apamtoi~k TvtL itsevr eja PLL..
Date/Time.swA'Pe. -Y aA-.
'I-VA q1)01,;3 oqls-r-
-24c?.uz3 4 6~L-ot'a-3t ETS-3 3~E O~-~
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
SETS Whole Effluent Sample Receipt Log Page J.Lw
- Sarnple temperature performed using Sample ReceivingThermometer. SN 122164697 UA) 0 Date Time Received Received
- Sample Project Sample Reeied Rceve b
ro
~
evt.I~
ume nmbrSample name and description State Comments Received Received by from Temp. ftC) number number 09-20-13 0940 K. Keenan Fed - Ex 0.8 9268 130920.01 Ahoskie WWTP NC 09-20-13 0940 K. Keenan Fed - Ex 1.5 9269 130920.02 Albemarle - Long Creek WWTP NC 09-20-13 0940 K. Keenan Fed - Ex 2.0 9270 130920.03 Carillon Building NC 09-20-13 0940 K. Keenan Fed - Ex 1.5 9271 130920.04 Cleveland WWTP NC 09-20-13 0940 K. Keenan Fed - Ex 1.3 9272 130920.05 Dunn/Blackriver WWTP NC 09-20-13 0940 K. Keenan Fed - Ex 1.2 9273 130920.06 Craven County Wood Energy NC 09-20-13 0940 K. Keenan Fed - Ex 1.5 9274 130920.07 High Point Care Center NC 09-20-13 0940 K. Keenan Fed - Ex 1.1 9276 130920.08 Wells Fargo NC 09-20-13 0940 K. Keenan Fed - Ex 1.5.
9277 130920.09 Bladenboro WWTP NC 09-20-13 1145 J. Sumner Duke Courier 0.6 9263 130920.10 Duke Energy - MNS WWCB 0.3 M NC 09-20-13 1320 J. Sumner TVA Courier 1.2/1.0 9261 130920.11 TVA - Sequoyah Nuclear Plant - Outfall 101 TN 09-20-13 1320 J. Sumner TVA Courier 0.9 9261 130920.12 TVA - Sequoyah Nuclear Plant - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
- ETS Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TM2NOO466*1-t
'00264SO Project#:.
AL k county: 10._
Outfall: 101 Dilution praration information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volumet(mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0
I.Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
I S.S1 I OAN" 0o.X Randomizing template:
Date and times organisms OR,.0- %- M0.0 Incubator number and were born between:'
shelf location:
Organism source:
A 1p o*. It Artemia CHM number:
CA* *"1io
-U Drying information for weight determination:
Transfer bowl information:
pH = "1.
S.U.
Date / Time in oven:
0%.2,%
Temperature =
- .j c Initial oven temperature: _
".V Average transfer volume:
Date / Time out of oven:
Final oven temperature:
loo *L Total drying time:
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 10
-I
,I, 4
110 A
t054 A-,
lb~ i-1.01 rb
'M1L-.G*.
O+4-I.5 3 OR-¢.
it\\%
\\C\\0.
161 dam
,,.Sl Qb~t' I*
M OVA't I.
.ISt A-.
043q 6
i M
u 1A Vd tARi. i I tQ1/612.17-
- a*.-m,
- WVV
ý7 0q Sol I-Control inf ormation:
Acceptance criteria Summary of test endpoints:
% Mortality:
- 01.
5 *20%
"7-day LCso 10.07.,
Average weight per initial larvae:
0.tas NOEC J
1b07.
Average weight per surviving larvae:
,. 6,4S 0.25m arvae LOEC
> 1067.
ChV
> 1007l.
IC25 I
iW age 32 of 105 SOP AT20 - Exhibit AT20.3, revision 07-01-12
- ETS Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date:
60,. t'l-. %
i i
Survival and Growth Data
- 1 Day
'CONTROL 10.8%
21.6%
A B
C D
E F
G H
I J
K L
0 ID 1O to ID to to ri b to 10 10 1 o 0
I'D 10
/b 1b 16 1 ID 1t 10 10b 10 10
/0 2
to 10 ID 10 tb 1%3 10 C 10 1C lo1 to 3
10 I0 10 to1 ot l(1 i0 to toI0 to 4
1 10 t0 10 to 1
o0 to I0 1(0 10 10
/0 5_,_
,.0 1
1f3 b
I)
(c0)0I0'0'0 6
ID
._l 10 to 10l
[
10 Lto 0 tu 0l to to 7
%UP_
to to 13 10 (10 I
b 10 lb
/1)
'0 t0o A = Pan weight (nag)
Tray color code::
Analyst: W 15-V-1.-5-9 1.39 15.qq 1569 915h fIS L
I07 115316 I16nI.
3I.
Date: CAA
-%I.
B = Pan + Larvae weight (mg)
Analyst:
VU7.2 aI.1N
.I.1 271d i
I..
z o
Z.
Date:
O9 i Z.5.L lq Z1.u1 LI.TI ZZ.
20.9 21.6D Z1.69 C = Larvae weight (mg) B - A Hand calculated.
6
'.5s
(,.#
L.is
(
.as 4 6
0,*(..4 iC.-.V
(
lot Analyst:
Weight per initial number of larvae (mag)
= C /Initial number of larvae Hand calculated.
Analyst:
A.
0*
0 4,
'a A,
I.v
'a
'a
'10
'7 01, b*
'D#
Average weight per Percent reduction initial number of from control (%)
0.
larvae (nig) j q
O. -
4.-
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
age 33 of 105 SOP AT20 - Exhibit AT20.3, revision 07-01-12
- ETS Page 3 of 6 Species: Pimephales promdas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date:
Survival and Growth Data Day 43.2%
86.4%
100%
M N
0 Q
R S
T U
V W
X 0
D
/0 to Do It
/0 O
C) t I t0 (0 I'
iO (0 1I lb ID 10 IQ 1I i.b I
0 2
10 10 t
o 10 1t I0 bO 1o3 to I 0 /10 0C I-0O 10 110 10 (0
10 10 4
1 1(
I O
10 It oo.
to to 5
10 U
0 10 0
10 10 1()
10 10 to I0 10I 6
10 to to 1(3 0 to to to t0 0 I
to 1C.
7 10
.0 to
'/
to to 10 to t
0 0
to 10 A = Pan weight (mgi Tray color code::
PAL q-Analyst:
K.41 j'I.i 15.55 i01 iS.l S.-0 It.1 153.3.1 14
% '1% 3.1i 15.00 Date:
oq. I S' M0 B = Pan + Larvae weight (mag)
Analyst:
ZO
)
l Zt)I1 X5 Z0. i3Z¶oZtS IjZq I~' Ci 10 Date: y Z
O*34 N
2106 ZI169 2.01 21.14 Z0 U-16Z I.o'0 C = Larvae weight (mrg) = B - A Hand calculated.
S
- 47. (Aq S.(
.i S.'6 v ib sm
".V
( U Analyst:
/.t...
Weight per initial number of larvae (mg)
=C/Initial number oflarvae Pb
),
Hand calculated.
I
't Analyst:
Average weight per Percent reduction initial number of from control(%)
O.S1
".'7.
O.62\\
"t \\.
O. it&
s.' 7.
larvae (mg)
Comment codes: c = clear, d = dead, fg fungus, k = killed, m = missing, skA= sick, smi= unusually small,,
1g = unusually large, d&r = decanted and returned, w = wounded.
e age 34 of 105 SOP AT20 - Exhibit AT20.3, revision 07-01-12
Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101, Non-treated Date:
Day 100% Intake Y
Z AA BB 0
0 10 ID ID to 10 to to 2
0 1
/Q 0 3
S*
4 O
a t I L ( I I (
5 I\\G
- Djr, 6
7, t
A = Pan weight (mg)
Tray color code::
It MIN Analyst:
CUD*'
IL1.ZR t..l Date:
Q9 - 1,*
B = Pan + Larvae weight (mg)
Analyst:
l.
Date:
o0"i.21.%1-V
.2-o 19.4 20.qZ2. 50 C - Larvae weight (mg) = B - A Hand calculated.
6 :;.6S Analyst:
Weight per initial number of larvae (mg)
= C/Initial number of larvae Hand calculated.
Analyst:
o'
[
Average weight per Percent reduction initial number of from control (%)
- 0.
V'*
A, 6.1.
larvae(mg)_______
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
(tage 35 of 105 SOP AT20 - Exhibit AT20.3, revision 07-01-12
SS Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated September 17-24,2013 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number.
9261 Not for Compliance Assessmnent Internal Laboratory QC Coneentrton(%)
Repticate lInitialnumberor Finda numberof larve A Pon eIght (mg)
B -Pan. + LAree Larvae weight (mg)
WeighlSoretrlgn Mean welght I Coettfiientof voartto Wdelht/ let/itia number Meansur~vvl Net.w.dghtltIntaltl.
c.rtenteof Percntredtlonfrem
- orn,
.Weight (me
-A-B number.to1ree Img)
Sl*rvlvg numberor 0&-
Wntl 0 0lla rWm(me)
(-1f ft.,,. (W numbeer.hatse v
aratin.o nmmetokt
(%t) tarte. tmg)
(eangmt(%) W 0,1)
,anatafto-)
A 10 10 15.46 21.22 5.76 0.576 t.576 B
10 to 15.32 22.33 7,01 0.701 0.701 Control C
10 10 15.23 21.14 5.91 0.625 9.0 0.591 100.0 0.625 9.0 Notaplicable D
10 10 15.19 21.52 6,33 0.633 0.633 E
10 10 15.39 22.14 6,75 0.675 0.675 F
(0 10 15.44 21.67 6,23 0.623 0.623 10.8%
G 10 10 14.56 21.40 6.84 0.684 0.650 5.3 0.684 100.0 0.650 5.3
-4.0 H
10 10 16.17 22.35 6,18 0.618 0.618 1
10 10 15.30 20.94 5,64 0.564 0.564 J
10 10 15.07 20.99 5.92 0.592 0.592 K
10 10 15.31 21.50 6,19 0.619 0.619 L
10 10 15.43 21.69 6,26 0.626 0.626 M
10 10
.14.88 20.38 5.50 0.550 0.550 43.2%
N 01 55 09
.20520.577 8.4 0.542 100.0 0.5"7 8.4 7.7 Nq 10 10 15.55 20.97 5.42 0.542 0.542 0
10 10 16.37 22.85 6.48 0.648 0.648 P
10 10 15.11 20.79 5.68 0.568 0.568 Q
10 10 15.02 20.39 5,37 0.537 0.537 R
10 10 16.51 21.86 5,35 0.535 0.581 9.0 0.635 S
10 10 15.35 21.65 6.30 0.630 3.'I T
10 10 15.47 21.69 6.22 0.622 0.622 U
10 10 14.74 20.13 5.39 0.539 0.539 100%
V 0.0 10 14.81 21.14 6,33 0.633 0.588 6.9 0633 100.0 0.588 6.9 5.9 W
10 10 15.02 20.76 5.74 0.574 0.574 X
10 10 15.00 21.07 6.07 0.607 0.607 Y
10 8
16.33 21.20 4.87 0.609 0.487 0.47 100% Intake Z
10 91 14.76 12.96 4.67 0.519 0.577 7.8 0.261 92.5 0.534 13.0 14.6 AA 10 10 1.576 292 6.16 0.616 0.616 BB 10 10 1
16.85 1
22.50 5.65 0.565 0.565 COatfall 101:
Dunnelt's MSD value:
PMSD:
Intake:
DanneUt's MSD value.
PMSD:
0.0757 12.1 0.0865 13.8 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a mneasure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA.(I 0th pere.ntile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) 30 0h6.
Lower and upper PMSD bounds were determined from the I 0th and 90th percentile, respectively, of PMSD data from EPA's WET Interiaboratoty Variability Study (1SEPA, 2001 a; USEPA, 2001 b).
USEPA. 2001a, 2001 b. Final Report: Intedaborstory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: sqn101.091713data.xlsx Entered by: J Sujiner Reviewed by:
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated September 17-24, 2013 a:T 6&ft..IT..tr51Un&
toein
.a
.Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
9/17/2013 Test ID:
PpFRCR Sample ID:
TVA/SQN101 End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Conc-%
I
. 2 3
4 D-Control 0.5760 0.7010 0.5910 0.6330 10.8 0.6750 0.6230 -0.6840 0.6180 21.6 0.5640 0.5920 0.6190 0.6260 43.2 0.5500 0.5420 0.6480 0.5680 86.4 0.5370 0.5350 0.6300 0.6220 100 0.5390 0.6330 0.5740 0.6070 Intake 0.4870 0.4670 0.6160 0.5650 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N.Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.6253 1.0000 0.6253 0.5760 0.7010 8.951 4
0.6376 1.0000 10.8 0.6500 1.0396 0.6500 0.6180 0.6840 5.280 4
-0.788 2.410 0.0757 0.6376 1.0000 21.6 0.6003 0.9600 0.6003 0.5640 0.6260 4.709 4
0.796 2.410 0.0757 0.6003 0.9414 43.2 0.5770 0.9228 0.5770 0.5420 0.6480 8.417 4
1.536 2.410 0.0757 0.5821 0.9129 86.4 0.5810 0.9292 0.5810 0.5350 0.6300 8.962 4
1.409 2.410 0.0757 0.5821 0.9129 100 0.5883 0.9408 0.6883 0.5390 0.6330 6.928 4
1.178 2.410 0.0757 0.5821 0.9129 Intake 0.5338 0.8537 0.5338 0.4670 0.6160 12.972 4
Auxillary Tests Statistic Critical Skew Kurt Shaplro-Wilk's Test Indicates normal distribution (p > 0.01) 0.92167 0.884 0.41106
-1.0649 Bartlett's Test Indicates equal variances (p = 0.69) 1.68483 15.0863 Hypothesis Test (1-tall, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
0.0757 0.12107 0.00327 0.00197 0.19804 5,18 Treatments vs D-Control Unear Interpolation (200 Resamples)
Point SD 95% CL(Exp)
Skew IC05 20.013 ICIO
>100 IC15
>100 1.0 IC20
>100 0.9 IC25
>100 IC40
>100 0.5 ICSO
>100 0.7 0.6 0.5 19 0.3 0.2 0.1 0.0 00 50 100 150 Dose %
Dose-Response Plot 0.8 0.7 0.6 0.5 0.4 90.3 0.2 0.1 0
9F 1-tall, 0.05 level of significance o
Q C
CO C
N S
tage 37 of 105 File:
iniOl 091l7l3data.xlsx Entered by: J. S er Reviewed by: __
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated September 17-24, 2013 "ETS 5
Envlronmental Testing Solutions. Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
9/17/2013 Test ID: PpFRCR Sample ID:
TVA/ SQN101 - Intake End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Conc-%
1 2
3 4
D-Control 0.5760 0.7010 0.5910 0.6330 10.8 0.6750 0.6230 0.6840 0.6180 21.6 0.5640 0.5920 0.6190 0.6260 43.2 0.5500 0.5420 0.6480 0.5680 86.4 0.5370 0.5350 0.6300 0.6220 100 0.5390 0.6330 0.5740 0.6070 Intake 0.4870 0.4670 0.6160 0.5650 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 0.6253 1.0000 0.6253 0.5760 0.7010 8.951 4
10.8 0.6500 1.0396 0.6500 0.6180 0.6840 5.280 4
21.6 0.6003 0.9600 0.6003 0.5640 0.6260 4.709 4
43.2 0.5770 0.9228 0.5770 0.5420 0.6480 8.417 4
86.4 0.5810 0.9292 0.5810 0.5350 0.6300 8.962 4
100 0.5883 0.9408 0.5883 0.5390 0.6330 6.928 4
- Intake 0.5338 0.8537 0.5338 0.4670 0.6160 12.972 4
2.055 1.943 0.0865 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.89243 0.749 0.44527 -1.5862 F-Test indicates equal variances (p = 0.74) 1.53061 47.4683 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.0865 0.13835 0.01674 0.00396 0.08559 1,6 Treatments vs D-Control.
Dose-Response Plot 0.8 0.7
.0.6 S0.5 O 0.4 0.3 0.2 0.1 0
.C 1-tail, 0.05 level of significance M
(0 0
J It
-O a~
N r
rn~p~o~
R.~hy Selleyt Keenn IflIt.II*
age 38 oF 105 File: sqnlO1 091713data.xlsx Entered by: J. Sumner Reviewed by:
OETS Page 5 of 6 Species: Plmephales promelas Client: TVA / Seauoyah Nuclear Plant, Outfall 101. Non-treated Daily Chemistry:
Date:
IA-i1-M IDay (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.)
0 2
Analyst 1
IA.
Con cent ratio m,,,,m.,n IParameter pH (S.U.)
nIA's DO (mrnL/
CONTROL Non-treated
ýConductivity
~
1 (jimbo/cm)
(mg CaCO3/L)
L
- Hardness (mg CaCO 3/L)
ROL__
-1.1
.300a I2
- Temperature (0C)
II Iad U
S DH (S.U.)
"AA* I II -I
- -4*'~a~-*------**--=~-
DO (mRlL)
II 10.8%
Conductivity (Limbos/M)
- Temnerature 00C "14.A I S~
(U.)
L 21.6%
Conductivity I
U (Igmhos/cm)
(00)4
. W a
I.-s _
a 7-
~
I 1.t
.U
.2..
.6.04 to nH (S.U.M 43.2%
DO (mg/b)
Conductivity I 4P (gii mos/cm)A4
- Temperature (o)
-Vi-p IPH (s.u.)
qIq DO (mg/b) 6; 86.4%
Conductivity uno/m
____________*Temsperature (00Q pH (S.U.)
f4:ni-
.DO (mg/L) 100%
Conductivity
-(ILm~hos/cm)
Is's___
- Alkalinity
- Hardness(0 (mg CaCO3IL) f
'4
- 10
- Temnerature (0C0 K.1) i 6H (S.U.)
~,110 DO (mg/L) elI
- 1 Conductivity Li L
M) 15s/cm 100%
- Alkal*nity
.tot Intake Ing CaCO3/L)
I*HardnessJ(mg CaCO3IL) 0
- TR chlorine (mg/L)
- Temperature (0C 14 Initial Final Initial Final Initial Final
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
,-Alkaljity_ rdness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet aPof 105 SOP AT20 - Exhibit AT20.3, revision 07-01-12
O:T Page 6 of 6 Species: Pimephales promelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. Non-treated Date:
O~-It.'-
I*
Clet:TA euoa Ncer lnLOtfl 01 ontete at:I I
Day (Analyst identified for each day. nerformed nil D.O. and conductivity measurements onlv.)
I Analyst F 3
I 4
I 5
6~i Concen-Parameter tration I________
J pH (S.U.)
DO (m/L) 0-~-----
Conductivity (ttmhos/cm)
CONTROL Non-treated 0*
- Alkainity (maz CaCO2%/L)
- -u.-~----.-
- Hardness (me CaCOVUL
- Temperature (%)
I0S DH (S.U.)
DO (mg/L) 10.8%
Conductivity__
(Amhoslcm)
ZAO V2-23 ~LO
- Temperature (10
-K.
C1
~.'A ~S AL. h-L
--BSA.
t PH (S.U.)
- 51 DO (mg U IL (jwihos/cm)
CUT LV
- Tempertur CC 43.2%
Cnductivity (pmhos/crn)
___4___
1_
- Temperature(O CC) vq A~0A L'..
DH (S.U.)
0 II -~-~~--
9 DO (mg/L) 86.4%
Conductivity 11.h "cm."
os
- TemDerature (PC I
II-
/,go~L pH (S.U.)
DO (mra/L) 100%
Conductivity (grahos/cm)
- Alkalinity (mg CaCQ3/L)
- Hardness (ngCaCO 3/L)
- TR chlorine (mg/L)
- Temuerature (00
.1 100%
Intake Conductivity
-(Iimhos/cm)
- Alkalinity
-(mig CaCO0/L)
- Hardness (mg CaCO3/L)
- TR chlorine (mg/L)
- Temperature (C)
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
ityt ardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet
_\\
SOP AT20 - Exhibit AT20.3, revision 07-01-12
- __.J a g e 4 0 o f 1 0 5.
0*
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated September 17-24, 2013 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number:
9261 Concentration Parameter Day 0 Day I Day 2 Da 3 Da 4
Da 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.95 7.73 7.85 7.54 7.92 7.69 7.94 7.51 7.99 7.68 7.98 7.70 8.07 7.63 DO (mg/L) 7.8 8.1 7.9 7.2 7.9 7.5 8.1 6.8 7.9 7.2 7.9 7.4 7.7 7.4 Conductivity (limhos/em) 311.A 308 0
- 323, 306 317 j,4P 318 306 -
Control Alkalinity (mg/L CaCO3) 61 60 61 t
Hardness (mgIL CaCO3) 92
- 9.
90 90 I
.. I d
M',
Temperature 0T) 24.8 24.6 24.8 24.6 24.8 24.7 24.8 24.7 24.7 24.5 24.9 24.7 24.9 24.7 pH (SU) 7.81 7.71 7.82 7.40 7.77 7.68 7.92 7.47 7.95 7.62 7.93 7.69 8.04 7.63 DO (Mn/L) 7.9 8.2 8.1 7.3 7.9 7.5 7.9 6.8 8.0 7.2 7.9 7.4 7.8 7.4 10.8%
Conductivity rnmhos/cm) 288 Q 289 2
293 285 292 j 296
- 295 R -
Temperature 24.9 24.9 24.8 24.7 24.8 24.7 24.8 24.7 24.7 24.4 25.0 24.5 24.9 24.7 PH (SU) 7.84 7.69 7.83 7.40 7.76 7.63 7.92 7.48 7.96 7.63 7.97 7.65 8.04 7.57 21.6%
DO (mrnL) 7.9 8.2 8.1 6.8 7.9 7.5 7.9 6.8 7.9 7.1 7.9 7.4 7.8 7.4 Conductivity (rtmhos/cm) 275 276 278 270 277 277 W.
278 277 Temperature "*C) 24.9 24.9 24.8 24.7 24.8 24.5 24.8 24.7 24.8 24.4 25.0 24.6 24.9 24.8 pH (SU) 7.80 7.69 7.84 7.40 7.68 7.64 7.92 7.48 7.95 7.60 7.96 7.65 8.04 7.58 DO (mgrL) 7.9 8.2 8.2 6.8 8.0 7.5 7.9 7.0 7.9 7.1 8.0 7.3 7.8 7.4 43.2%
Conductivity (inhos/cm) 241
£ 245 246 239.
242 W-N 243 247 Temperature ('Q 24.9 24.8 24.8 24.5 24.8 24.5 24.8 24.8 24.8 24.6 25.0 24.6 24.9 24.5 pH (SU 7.79 7.65 7.84 7.40 7.56 7.63 7.86 7.48 7.90 7.57 7.96 7.65 8.00 7.60 DO (mrJL) 8.0 8.2 8.2 6.8 8.0 7.5 7.9 6.9 8.0 7.0 8.0 7.3 7.8 7.4 Conductivity (pmhos/cm) 177 181 184 175 f' 178 180 180 Temperature 24.9 24.8 24.8 24.7 24.9 24.7 24.8 24.6 24.8 24.6 25.0 24.3 25.0 24.5 pH (S-7.71 7.65 7.80 7.38 7.50 7.61 7.80 7.42 7.89 7.59 7.91 7.65 7.99 7.55 DO rmnfL) 8.1 8.2 82 6.8 82 7.5 7.9 6.9 8.0 7.0 8.0 7.3 7.9 7.4 Conductivity (pmhoslcm) 155 157 157 157 153
-M 156 j
157 155 100%
Alkalinity (mg/L CaCO3) 61 *.
60.W.,
60 I M 60 Hardness (mg/L CaCO3) 66 68 a,
64 TotalResidual Chlorine (mrnL)
<0.10 i
<0.10I J 4R
<0.10 Temperature (OC) 25.0 24.7 24.9 24.7 25.0 24.7 25.0 24.7 24.9 24.7 25.0 24.5 25.0 24.8 pH (SU) 7.70 7.65 7.79 7.37 7.45 7.66 7.80 7.49 7.87 7.68 7.93 7.69 7.86 7.65 DO (miJL) 8.1 8.1 8.2 7.0 8.2 7.5 7.9 6.8 7.9 7.0 8.0 7.5 7.9 7.4 Condnctvity (Imhos/em) 152 155 156 X,;V 151V r
156 :
158 158 100% Intake Alkalinity (mgfL CaCO3) 61 61 60I I
Hardness (mg/L CaCO3) 60 70 Total Residual Chlorine (ig/L)
<0.10
- t.
T
<0.10
"-* i
- t.
<0.10 4
t..
'j
'/
ITemperature M 25.0 24.6 24.9 24.7 25.0 24.6[
24.9 24.6 24.9 24.4 25.1 24.5 24.9 24.7 File: sqn101_091713chem.xlsx Entered by: S Taylor Reviewed by:
- ETS:
0,Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Project #:
6%'l County: Hamilton Outfall: 101 T
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080.
2160 2500 Diluent volume (mL) 2230 1960 1420 340 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information:
Test information:
Organism age:
< 24-hours old Randomizing template color:
f Date and times organisms were born 4, V%.
1I'S 4"oTO O 041 Incubator number and shelf between:
Ilcaton:
Culture board:
A -
location:
Replicate number:
1 2
3 4
5 16 17 16 9
110 YTbth Culture board cup number:
kl I10 olb -W I
.'V -0.
YWT1batch:
-J5 Transfer vessel information:
pH = 1).!
S.U* Temperature=
- (
Selenastrum batch:
Average transfer volume (ml):
Se e ubn'a tVrm I
Daily renewal information:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake termination time 0
0A..tr1.1 oAq4 td.tq-:S I-Ocl,.o.
I I,. Ot 2
- m. vs 4N A -A.i., %%y1 l u l 3__
01.______
160l~ill.
4 A
4
?A.2---!
0OLS
"~ru
%5 No
-t
- is~io.
-M 6
0q'UV11d 7 Di C_
- 4 094*1
+/-1 Control Information:
Summary of test endpoints:
_ Control-I Control-2 Acceptance criteria
% of Male Adults:
- 0..
- 07.
<520%
7-day LC50
> 1067.
% Adults having 3"' Broods:
I 001 1 007.
>80%
NOEC 1.
% Mortality:
0?.
20%
LOEC
>101, Mean Offspring/Female:
2t L
>15.0 offspring/female IChV
> 00 7,
%CV:
¶27.
<40.0%
IC 25
'; I007n nd.,2of 10
- ETS
- ""*""*"*'*Page 2 of 7 Species: Ceriodaphnla dubia Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date:
o*'*s-"
V*
CONTROL-1 Survival and Reproduction Data I
Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Young produ-.--
O
,C)CeI d
Adult mortality
\\..."
21 Young produced L..[
0.
I
. I,__
Adult mortality 3
Young produced I I..
oil I
101._ ".Q.. I 0
.. _ 1 0 Adult mortality s-
-I_~F 4
~ ~ L 4~
4 l Young produced LA IS. I IC V W 0 Wi l
1 U J Adult mortality 6
J Young produced C)
Adult mortality J "
I 7j Young produced 14 A T
T I
Total young produced g
3C)
"LA
- 1.
il Xi U.
2.1 3b I Final Adult Mortality L
C.
T.
X for 3r Broods X-z i
-s Note: Adult mortality (L = live, D dead). SB - split brood (single brood split betweea two days), CO - carry over (offsprng carried over with adult during transfer).
Concentration:
% Mortality:
Mean Offspring/Female:
L*
CONC: 10.8%
Survival and Reproduction Data Replicate number Day 1
213
.4 5
6 1
7 8
9 10 1
Young produced Vi-I) jAdult mortality F&
1.._.
.L__
Young produced c
i Adult 1C _O_oI 0,
I,0, 0i O 3 Y mortality 1
1 ---
1 1
4 Young produced
'U X
I Adult mortality
-1
'- I L
5 Young produced Ito VL{ 0~ f -L I0 1?
H :
Adult mortallt
-l o
i'I lt 6
Young produced C
0 10 1*
o io 1
1 Adult mortality L
L 7
Young produced 14 i1%A 1%
i i-t Total young produced j1
- zsi
- I.
.3\\
10*
I-4\\
Final Adult Mortality I t.
c..
Note: Adult mortality (L.- live, D - dead), SB-split brood (single broad split between two days), CO carl ov r
ned overwith adul during transfe Concentration:
age 43 of 105
% Mortality:
07.*
Mean Offspring/Female:
% Reduction from Control-1:
Moraliy:,
SOP ATI 1 - Exhibit AT11 2, revision 07-01-12
@wETS Page 3 of 7 Species: Ceriodaphnla dubla Client: TVA / Sequoyah Nuclear Plant. Outfall 101 CONC: 21.6%
Surv Date:
A G.
ival and Reproduction Data Replicate number
__Da*,
1 2
3 4
5 6
7 8
9 10 1
Y oung 7produeed
[Adult mortality i
42 Young produced
- Young produced c)
_n__*___
Adult mortality L
L "
l ii 1 Young produced010 Adult mortality 1
_-° I
Y Young produced t""
Adult mortality L_._
_____J Co6nrain Yon!rdce
ý mo*duta lortali 7
Young producedMean %sin/Fale I
Total young produced Survival aRpdi'o.n 3A t
S1 L.
____FAdult mortality Mort y
____1ungr~dee I
I. *I*]*]%
0'*
3 N Adult morta_
(
_sp I
t d
i c
I i
- l.
Ii 4 I
Concengtprtion:
T aYoungproduced
.I.
IX.
I___
____~
F MAdult mortality A ut~tlt.
0_O._%
Motliy
.Mean Offspring/Female:
I l
8 I
.. 1
% Reduction fMrro Control-i:
- S147.
Ii coNc: 43.2%
Survival and Reproduction Data I
______Replicate number 1Young produced
-0 C)H ic If)c)I I
Adult mortalityl 2
Young produced 13 01 01 01 C) 0,0
__fAdult mortality k__ LL I.-_L kJ
%- L__
k__
t-3 Young produced I.. C). 1 01C n
16101o1 I
C fAdult mortality L_
~
I L~ I:
___L.
L__
L 4I Young prdue "A
1 S1 6 Adult mortality
\\__
I~
tý I*
i'-i SYoung produced I_
FL5 O 1 IIo
__fAdult mortality I
- 1.
4 4
ij L]7?jL tj 7
Young produced to t4 l-I.!~
je Totl yungprouce3I0 30 132-.
- 32.
3 251 3A Ist 21 Final Adult Mortality 1
0 Z:-: -=_
X.-2T Note. Adult mortality (L - lie, D - dead),
SB - split brood (single brood splilt between two days),
0O o
n-ver (offtivinsi caruded over with adult during transfer).
[Concentration:
J1%
Mortality:
7 Mean Offspring/Female:
avtT%,-*k I 1.
.tage 44 of 105
% Reduction from Control-I: 1 :
I SOP ATI 1 - Exhibit ATI 1.2, revision 07-01-12
a:
- ETS
- Fou/nt.IT.,5ae*d J tlowkIr..
Species: Ceriodaphnla dubia Client: TVA / Sequoyah Nuclear Plant. Outfall 101 CONC: 86.4%
SuM' Page 4 of 7 Date:
o0rr1"l" ival and Reproduction Data Replicate number Day 1
2 32 1
4 5
6 7
8 19 10 I
Young produced
'(n'_I p
p p
I I
I Adult mortality
'-I I
I....
2 Youngproduced I nI.C
__JAdult mortalit
]
\\
I '- J-.-
-1'1 3
Y oung produced 1o 01 1
10~ 0) ni 0
~
1 4 1 Young produced I I
I l IŽ 1
I '.
1_
'1 I Adultnmortality IL-I '-
I '-
1 I
"-'--i " I "- I"--
5 J Young produced 1
Adult mortality Itl
"_ I
' l I 6
Youngproduced
. olo~o L
1Adult mortality I*
1*
Total young produced j "
L 4 I.,
I Final Adult Mortality j
'.. 'tz.,
Note: Mull mortality (L - live, D dead), SB = split brood (single brood split between two days), CO = car over (offspng carred over with adult durin sf e
errt.
Concentration:
% Mortality:.
O17 Mean Offspring/Female:
j
% Reduction from Control-1:
CONc: 100%
Survival and Reproduction Data Replicate number Day 1 1 2
3 4
5 6
7 8
9 10 1
Young produced 0
0____
)
C)
Adult mortality L
\\.-
k
.2 Young produced
[
- 0)
C ICi
- 0)
)
Adult mortality
- I
'=--
I L
I 3
Young produced L oJ o,!
I I
oI o
Adult mortality IL-1 I
4~
Young produced I~~
wi i S..
s I
J YopueAdult mortality L_
j.....
I I
5 Young produced 1l']
'L.
"j4 I J4* [
]VL.
L. V'tI\\
JAdult mortality 1~_ \\_ -4
-['-
'-I'-
6 Young produced C)
FO Adult mortality L-7 Young produced I I I Ot I
t It I(.
Total young produced is 3J as 33 56 at 11 212 Final Adult Mortality J-.....
- k.
. I Note: Adult mortality (I. = live. D - dead), SB = split brood (single brood split between two days), CO = carry over (ofbpritn carried over with adult dut transfer).
I Concentration:
I
.% Mortality:
Mean Offspring/Female:
I *. L.
% Reduction from Control-i:
- l.
SOP AT] I - Exhibit AT 11.2, revision 07-01-12 Page 45 of 105
PoETS 40 tM-u'..M 'dU* ft Page 5 of 7 Species: Cerlodaphnla dubla Client: TVA / Sequoyah Nuclear Plant Outfall 101 CONTROL-2 Surv Date:
00,
ival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Young produced
- 0) 1
)
0 o"
o o
o C) n Adult mortality X.
Young produced Q
I l I
C Do Adult mortalityI
_-I
--_--I_
-I 3
Young produced
- 0.
O, O
l 4
Adult mortality I
4 Young produced 1
Adult mortality K-K-1.
l_"l l-5 Y ou ng pr odu ced I f,-
C) l. I (
I
- l
,__Adult mmortlity
___1
_1 5
Yung produced i
7 Young producedL I
Total young produced
[Li*0 "Sb Toti'
=
10
.0
....o I0 Final Adult Mortality
_.t.
L
[ L j
t I
X o
r I~o II
'I I
X for 3
('
Broods
- dead) si brood (sin.
r
- t.
O r oe(o.
' i Note: Adult mortality (L = live6 D0- dead), SB =split brood (single broad split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
Mean Offspringemale:
I Survival and Reproduction Data CONC: 100% Intake
- I.
I _Replicate number.
Day 2
-3 1
4 5
6 7
8 9
10 1
Young produced f 2
n)
I) 6 10 Adult mortality X._.
L--
2 Young produced l
Adult mortality I1 LI
'-I
'I
'I LI
[ Young produced 0--I K31 j
C0 O 1
I3 Adult mortalit Ity IL--IL.1L I U-F V I-LILI b
.4 Young produced i V
I FWI VW si L-- tX" Adult mortality j
I'--
s Young produced jj Q10
[
%-L V.110 Ij09 12b~
j1 Adult mortality
\\.-
i I
\\-
_\\-
6 Young produced C) 0
~~i L~
0 1 00 f
Adult mortality LLI
.7 Young produced.. 2.
Is.+/-..
~
IS
-j
-S I Total young produced so
~
~
~
Final Adult Mortali-Note: Adult mortality (L - live. D - dead)l Su - split brood (single brood split between two days), CO can over (offpsring earnied over with adult during transfr).
Concentration:
% Mortality:
07.
Mean Offspring/Female:
,32.1
% Reduction from Control-2:
-12.* 7
(
Page-46 of 105 SOP AT I1 - Exhibit ATI11.2, revision 07-01-12
M
- ETS 4 &v ironmm nt lTeu ing Su l*ua n, Inc.
4Control-I.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated September 17-24, 2013 Verification of Ceriodaphnia Reproduction Totals 86.4%
CiA Day Replicate number 1
2 3
4 5
6 7
8 9
10 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 4
3 4
4 5
4 3
4 4
39 5
10 12 11 10 10 13 10 10 10 11 107 6
0 0
0 0
0 0
0 0
0 0
0 7
14 14 15 114 14 17 113 15 16 1
4 Total 28 30 29 28 28 35 27 28 30 28 291 10.8%
D Relicate number D
1 2
4 0 0
7 8
9 1 0 Total 2
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 4
4 3
4 4
5 4
4 4
41 5
12 10 12 10 11 12 10 10 10 11 108 6
0 0
0 0
0 0
0 0
0 0
0 7
15 15 14 15 14 16 16 14 14 14 147 Total 32 29 30 28 29 32 31 28 28 29 296 21.6%
Day ~R licate numberToa 1
0 0
0 0
0 6
0 0
0 0
0o 1
0 0
0 0
0 0
0 0
- 0.
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
3 4
4 5
4 5
5 4
4 42 5
10 13 11 11 10 12 12 11 10 10 110 6
0 0
0 0
0 0
0 0
0 0
0 7
15 116 114 118 15 16 115 15 15 17J 156 Total 29 32 29 33 30 32 32 31 29 31 308 43.2%
Replicate number Total Day 1
2 3
4 5
6 7
8 9 110 1
0
.0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
6 4
4 4
5
- 4.
5 4
5 45 5
12 10 12 11 12 12 10 10 10 12 111 6
0 0
0 0
0 0
0 0
0 0
0 7
18 14 14 17 16 17 15 19 17 16 163 Total 34 30 30 32 32 34 29 34 31 33 319 Day
~Re plicate numberToa DY 1 2
3 4
- 5.
7 8
9110 Toa 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0' 0
0 4
4 4
4 5
5 5
5 4
4 5
45 5
12 10 12 11 13 12 12 10 11 11 114 6
0 0
0 0
0 0
0 0
0 0
0 7
15 17 14 17 18 18 17 15 19 16 166 Total 31 31 30 33 36 35 34 29 34 32 325 100%° Rficate number Total Day 1
2 3
4 5
6 7
8 9
10 1i 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 6
4 5
5 4
5 5
47 5
11 12 12 10 13 10 12 12 12 11 115 1 6 10 0
0 0
0 0
0 0
0 10 10 7
18 19 16 19 18 18 19 15 16 16 174 Total 33 36 32 35 35 33 36 31 33 32 336 Control-2 Replicate number Total Day 1
2 3 14 15 6
7 8 19 10 TO 1
0 0
0 0
0 0
0 0
0 0
0
- 2.
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
3 4
4 4
4 5
41 5
10 10 10 12_
10_L 13 11 I 11 12 110 6
0 0
0 0
0 0
0 0
0 0
0 7
14 13 14 14 16 13 15 14 14 13 140 Total 28 28 28 30 29 30 30 29 29 30 291 100% Intake D
Replicate number Total 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0
-0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 5
5 4
4 45 13 10 13 12 12 10 10 13 11 12 116 6
01 0
0 0
0 0 10 10 10 0
0 7
1 17 117 118 15 17 16 19 15 18 14 Total 1351 31 37 31 33 30 34 331 33 30 File: sqn 101_O91713data.xlsx Entered by: J. Sumner Reviewed by.
CO 0
C Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated September 17-24, 2013 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
9261 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from
(%)
1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variation (%)
control (%)
Control - 1 28 30 29 28 28 35 27 28 30 28 100 29.1 7.8 Not applicable 10.8%
32 29 30 28 29 32 31 28 28 29 100 29.6 5.3
-1.7 21.6%
29 32 29 33 30 32 32 31 29 31 100 30.8 4.8
-5.8 43.2%
34 30 30 32 32 34 29 34 31 33 100 31.9 5.8
-9.6 86.4%
31 31 30 33 36 35 34 29 34 32 100 32.5 7.0
-11.7 100%
33 36 32 35 35 33 36 31 33 32 100 33.6 5.3
-15.5 Control - 2 28 28 28 30 29 30 30 29 29 30 100 29.1 3.0 Not applicable 100% Intake 35 31 37 31 33 30 34 33 33 30 100 32.7 6.9
-12.4 Outfall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
1.942 6.7 1.378 4.7 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10 ' percentile) = 13%.
Upper PMSD bound determined by USEPA (9 0 'h percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interiaboratory Variability Study (USEPA, 2001 a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
File: sqn1O1_091713data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Sumner Reviewed by:
-r
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated September 17-24, 2013
- [
T S Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
9/17/2013 Test ID: CdFRCR Sample ID:
TVA / SON101 End Date" 9/2412013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
CD-Celiodaphnia dubia Comments:
Non-treated Conc-%
1 2
3 4
5 6
7 8
9 10 Control-1 28.000 30.000 29.000 28.000 28.000 35.000 27.000 28.000 30.000 28.000 Control-2 28.000 28.000 28.000 30.000 29.000 30.000 30.000 29.000 29.000 30,000 10.8 32.000 29.000 30.000 28.000 29.000 32.000 31.000 28.000 28.000 29.000 21.6 29.000 32.000 29.000 33.000 30.000 32.000 32.000 31.000 29.000 31.000 43.2 34.000 30.000 30.000 32.000 32.000 34.000 29.000 34.000 31.000 33.000 86.4 31.000 31.000 30.000 33.000 36.000 35.000 34.000 29.000 34.000 32.000 100 33.000 36.000 32.000 35.000 35.000 33.000 36.000 31.000 33.000 32.000 Intake 35.000 31.000 37.000 31.000 33.000 30.000 34.000 33.000 33.000 30.000 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD.
Mean N-Mean Contrd-1 29.100 1.0000 29.100 27.000 35.000 7.845 10 31.250 1.0000 Control-2 29.100 1.0000 29.100 28.000 30.000 3.009 10 10.8 29.500 1.0172 29.600 28.000 32.000 5,330 10
-0.589 2.287 1.942 31.250 1.0000 21.8 30.800 1.0584 30.800 29.000 33.000 4.791 10
-2.002 2.287 1.942 31.250 1.0000 43.2 31.900 1.0962 31.900 29.000 34.000 5.809 10
-3.297 2.287 1.942 31.250 1.0000 86.4 32.500 1.1168 32.500 29.000 38.000 6.994 10
-4.004 2.287 1.942 31.250 1.0000 100 33.600 1.1546 33.600 31.000 36.000 5.287 10
-5.299 2.287 1.942 31.250 1.0000 Intake 32.700 1.1237 32.700 30.000 37.000 6.921 10 Auxllia!y Tests Statistic Critical Skew Kurt KolmogOrOV D Test indicates normal distribution (p > 0.01) 1.01531 1.035 0.59456 0.42645 Bartlett's Test Indicates equal variances (p = 0.72) 2.83833 15.0863 The control means are not significantly different (p = 1.00) 0 2.10092 Hypothesis Test (1-tall 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
1.9418 0.06673 30.11 3.60556 6.7E-06 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples)
Point SD 95% CL Skew IC05
>100 IC10
>100 IC15
>100 1.0 IC20
>100 0.9 IC25
>100 0.8 IC40
>100 0.7 IC50
>100 0.6 0.5 r 0.4 0.3 0.2
.0.1 0.0
- -S S
S S
-0.1
.0.2
-0.3 1.
0 50 100 150 Dose %
Dose-Response Plot 40 35 30 1-tail, 0.05 level 25 of significance 20 15 10 5
0 O
C6 File: sqnI0l_092713dataxlsx 1Entered by J. Sumýer age 49 of 105 e*_iewed by
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated September 17-24, 2013 SET (bEnvirrnmentalTesting SoI~lixtos, Imc Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
9/17/2013 Test ID: CdFRCR Sample ID:
TVA / SQN101 - Intake End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Non-treated Conc-%
1
.2 3
4 5
6 7
8 9
10 Control-1 28.000 30.000 29.000 28.000 28.000 35.000 27.000 28.000 30.000 28.000 Control-2 28.000 28.000 28.000 30.000 29.000 30.000 30.000 29.000 29.000 30.000 10.8 32.000 29.000 30.000 28.000 29.000 32.000 31.000 28.000 28.000 29.000 21.6 29.000 32.000 29.000 33.000 30.000 32.000 32.000 31.000 29.000 31.000 43.2 34.000 30.000 30.000 32.000 32.000 34.000 29.000 34.000 31.000 33.000 86.4 31.000 31.000 30.000 33.000 36.000 35.000 34.000 29.000 34.000 32.000 100 33,000 36.000 32.000 35.000 35.000 33.000 36.000 31.000 33.000.32.000 Intake 35,000 31.000 37.000 31.000 33.000 30.000 34.000 33.000 33.000 30.000 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Control-1 29,100 1.0000 29.100 27.000 35.000 7.845 10 Control-2 29,100 1.0000 29.100 28.000 30.000 3.009 10 10.8 29,600 1.0172 29.600 28.000 32.000 5.330 10 21.6 30.800 1.0584 30.800 29.000 33.000 4.791 10 43.2 31.900.
1.0962 31.900 29.000 34.000 5.809 10 86.4 32,500 1.1168 32.500 29.000 36.000 6.994 10 100 33.600 1.1546 33.600 31.000 36.000 5.287 10 Intake 32.700 1.1237 32.700 30.000 37.000 6.921 10
-4.691 1.796 1.378 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94803 0.868 0.54828 1.12829 F-Test indicates unequal variances (p = 9.28E-03) 6.68116 6.54109 The control means are not significantly different (p = 1.00) 0 2.10092 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Heteroscedastic t Test indicates no significant differences 1.37814 0.04736 64.8 2.94444 1.8E-04 1.18 Treatments vs Control-2 Dose-Response Plot 40 35 30 o 25 5 20 0
15 10 5
0 8.
1-tail, 0.05 level of significance 8
N C.,
0GO 0
0 2as C
(tage 50 of 105 File: sqnl 0Il091713data.xlsx Entered by: J. Sunier Reviewed by:
i
.w IETS S6 Page 6 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant. Outfall 101 Daily Chemistry:
Date:
CA -In" \\*
Day (Analyst identified for each dy, performed pD D.C. and conductivity measurements only.)
0 1
2 Analyst I J-Concentration IParameter PH (s.u.)
DO (mg/L)
Conductivity "IF CONTROL
- Alkalinity (mg CaCO3/L)
- Hardness (mg CaCO,/L)
- Temperature (00 i
4 4
DH (S.U.)
4 I DO (mW/L) 10.8%
Conductivity (umhos/cm!
4
- Temnerature 00C%
DO (m&/L)
- 1.
AIIIII m
21.6%
Conductivity (jirrhos/cm) 2j(
Te eatr.
0
.0
- Temperature (°Cm "L.o
"'K. -a nH (S.U.'
_A o 43.2%
DO (mg/L)
A i I.'
Conductivity (jimhos/cm AL 1L
- Temperature (°C)
-15.0 S
P DH (S.U.)
DO (mUL)
OLD 86.4%
Conductivity (Amhos/M)
I 1I13
- Temnerature (0C)
"S. £
- PH (S.U.)
~
DO (mg/L)
- Conductivity 155' (iunhos/cm) 100%
- Alkalinity (mag CaCO3IL)
- Hardness (mg CaCO3IL)
- TR chlorine (mg/L)
- Temperature (00 I.
PH (S.U.)
O DO (mg/L)
Conductivity 100%
- Alkalinity Intake (mg Ca00 3/L)
- Hardness
- TR chlorine (mg/L)
- 0. 10
'Temperaturej (0)
.2 A21.
1134 (Po
'4 40.1 to 11S.0
.J nal l
Initial Final InitiaHia Initial
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual pWorine perfobr;nd by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
j.
51 of 105 SOP ATI 1 - Exhibit AT 11.2, revision 07-01-12
OTTS 0
E"-WTmg$dur-1ý Page 7 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant. Outfall 101 Date:
c*1-i4211 I
Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.)
I m
I I
I 4
I 5
I 6
Analyst Concen-Parameter tration
-Um DHl (S.u.)
--I -
DO (m F.L)
Conductivity (Umhos/cm)
CONTROL (mg CaCO3/L)
- Hardness (mg CaCO3/L)
- Temnerature (CC) pH(S.U.)
.Z DO (mg/L)
.0 10.8%
Conductivity
- Temperature (Q0 LV *L
.0 4.1 PH (s.u.)n ck 7 DO (mg/L) 21.6%
Cond~uctivity I-~
(izmhos/cm)
- Tel erature (0 pH (S.U.)
t ii 4
1I~
DO (mg/L)
"7.9 gb.4 43.2%
Conductivity
[2 9 1 ZIA
- Temperature (0C)
-L'.9
-~4. 'I "I.,g "I.0 S
S
- ~
ii U
il~
i dl pH (S.U.)
-. %to O'bo
-I.k 3 DO (mg/L) 119
]_1 Z
ýl q
11 11 86.4%
Conducivity I-.s j1o Al k
1-7K
- Temperature (00)
-M A p
Il-p
-II U
DnI (S.U.)
DO (mg/L) 100%
Coductivity (jimhos/cm)
- Alkalinity (mg CaCOAL)
- Hardness (mg CaCO3 Q)
- TR chlorine (mg/L)
- Temperature (0C)
U
."1.
~.¢....O Initial Final Initial Final Initial Final 100%
Intake Conductivity
_(;mhoslcm)
- Alkalinity (mg Ca003/L)
- Hardness (mg CaC0 31)
- TR chlorine (mg/L)
- Temperature (00 a
Initial Final
- Temperatures perfbrmed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal IInformation table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
t age 529of 105
- n+SOP ATI 1 - Exhibit AT 11.2, revision 07 12
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated ISeptember 17-24, 2013 a 1)Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test S0 EPA-821-R-02-013, Method 1002.0 (D.
- Daily Chemical Analyses (i*
Environmental Testing Solutions, Inc.
Project number:
9261 Concentration Parameter Day 0 Day 1 Day 2 Dae 3 Day 4 Da 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.95 7.80 7.85 7.80 7.92 7.89 7.94 7.88 7.99 7.90 7.98 7.91 8.07 7.81 DO (mg/L) 7.8 8.1 7.9 7.8 7.9 7.7 8.1 7.9 7.9 7.7 7.9 7.8 7.7 7.9 Conductivity (Amhos/cm) 311 308 323 306,
317 318 306 e&W-:TT Control Alkalinity (mglL CaCO3) 61 1
6 V4-60 W AO M..
61 Hardness (mg/LCaCO3) 92
.t 90 90 R A247 Temperature CC) 24.8 24.9 24.9 24.8 24.7 24.9 24.8 25.1 24.8 24.9 24.8 24.8 24.9 25.0 PH (SO 7.81 7.81 7.82 7.81 7.77 7.91 7.92 7.89 7.95 7.92 7.93 7.93 8.04 7.83 DO (mi/L) 7.9 8.1 8.1 7.8 7.9 7.7 7.9 8.0 8.0 7.7 7.9 7.7 7.8 7.8 10.8%
Conductivity (bmhos/cm) 288 j 289" 293 285 R.
4,¶ 292.
296 N-R-TM-;-
295..
Temperature (oC 24.9 25.3 25.0 24.8 24.7 25.0 24.8 25.0 24.9 25.0 25.0 24.7 25.0 25.2 PH (SU) 7.84 7.81 7.83 7.80 7.76 7.91 7.92 7.90 7.96 7.93 7.97 7.94 8.04 7.85 DO (mgrL) 7.9 8.2 8.1 7.9 7.9 7.7 7.9 8.1 7.9 7.8 7.9 7.7 7.8 7.8 21.6%
Conductivity wtmhos/cm) 275 U 4i 276 V 278 20 2 277 27 278 277 Temperature (°C) 25.0 25.0 25.0 24.9 24.8 25.0 24.8 25.0 24.9 25.0 25.0 24.9 25.0 25.0 pH (SU) 7.80 7.81 7.84 7.80 7.68 7.92 7.92 7.89 7.95 7.93 7.96 7.93 8.04 7.86 43.2 DO (mgL) 7.9 8.2 8.2 7.9 8.0 7.7 7.9 8.2 7.9 7.8 8.0 7.7 7.8 7.8 Conductivity (Inmhos/cm) 241 I?-Lr 245 Zi 246 239 242 243
- 247 Temperature (°C) 25.0 24.8 25.0 24.9 24.8 24.9:
24.9 24.9 24.9 25.0 25.0 24.7 25.0 25.1 PH (SU 7.79 7.82 7.84 7.79 7.56 7.92 7.86 7.88 7.90 7.88 7.96 7.88 8.00 7.83 DO (m-,)
8.0 8.3 8.2 8.0 8.0 7.8 7.9 8.2 8.0 7.9 8.0 7.8 7.8 7.9 86.4%
Conductivity (lwmhos/cm) 177 %
181 U-7 184 175 178 /
rt.*
180 @
180 Temrnerature (°C) 25.0 25.1 25.0 24.9 24.8 24.9 24.9 24.9 25.0 25.1 25.0 24.7 25.0 25.1 pH SU) 7.71 7.81 7.80 7.79 7.50 7.92 7.80 7.86 7.89 7.87 7.91 7.92 7.99 7.82 DO(meL) 8.1 8.3 8.2 8.0 8.2 7.8 7.9 8.2 8.0 7.9 8.0 7.8 7.9 7.9 Conductivity (wmhos/cm) 155 a
3 M,. _
157 157 V 153 156 ***3i 157.
155 A
,R-,TQ 100%
Alkalinity (mg/L CaCO 3) 61 60 60 W RV 9.I Hardness (mg1L CaCO3 )
66 i
68 64 Total Residual Chlorine (ragL)
<0.10...
<0.9I04
<0.10 Temperature 0°C) 25.1 25.0 25.1 25.0 25.0 24.9 25.0 25.0 25.0 24.7 25.1 24.7 25.0 25.3 PH (SU9 7.70 7.81 7.79 7.77 7.45 7.91 7.80 7.85 7.87 7.94 7.93 7.92 7.86 7.83 DO (mg/L) 8.1 8.3 8.2 8.01 8.2 7.8 7.9 8.2 7.9 7.9 8.0 7.9 7.9 8.0 Conductivity (pmhos/cm) 152 155 f 156 151 1
156 158 158 100% Intake Alkalinity (mg/L CaCO3) 61 j
K 61
- 60.
OH M
Hardness (mg/L CaCO,)
60 M rN L.1.
62 70.J-3 7
Total Residual Chlorine (mg/L)
<0.10.
<0.10
<0.10
. y
,. j Temnperature (°C) 25.0 24.9 24.9 25.0 24.9 24.9 25.0 24.8 24.9 24.8 25.1 24.8 25.0 24.9 File: sqniOl_O91713chem.xlsx Entered by: S. Taylor Reviewed by:
- ETS 0
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Seqyovah Nuclear Plant NPDES #: W "M 0'7OAS Project#:
O.l
\\LL County:
Outfall: 101 Dilution drearation information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2230 1960 1420 340 0
Interferences.
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
it.
06-b Randomizing template:
Date and times organisms 000- %06 " 000 Incubator number and were born between:
I shelf location:
Organism source:
"V.Y-9At
' 00"
.1 Artemia CHM number:
CVlO Drying information for weight determination:
Transfer bowl information:
pH = I.V1 S.U.
Date / Time in oven:
oorz'b %0!S Temperature =C g
°C Initial oven temperature:
Average transfer volume:
Date / Time out of oven: tALS. rb tOts Final oven temperature:
k0 "(
Total drying time:.
.1____
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 00 3 Oet.,i*.1.4 0"1A
..4..
-I
'1 I' s oi1,.
13 it3Oii.
ii di 14'.J3 41'-
(oo*
7d M(.*...1, tori.
- II 1&A,
.,L oi.(.,_
5_
OV o-el A10 1q~
- 10.
-150iU-I%
6 MIA. 1' 01bo At lboo We~
I' kNIA 20.11 1300iu -IL10-i-11 --A I I 0i, -A-15 oi%3 Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
- 01.
<20%
7-day LC5s
> 106'"1.
Average weight per initial larvae:
Ž025glava NOEC Average weight per surviving larvae:
O *
- 0.25mg/Ivae LOEC
>I Doi, IChV
>1 rti.
IC25 r-3
) Co.
Page 54 of 105 SOP AT20 - Exhibit AT20,3, revision 07-01-12
IET Page 2 of 6 Species: Pimephales promelas Client: TVA/ Sequoyah Nuclear Plant. Outfall 101, UV-treated Date:
oat - lxi %I2 Survival and Growth Data Day CONTROL 10.8%
21.6%
A B
C D
E F
G H
I J
K L
0 10 It to IO D
0 II 10 10 to 10 1 f I 1
10 io Vt to 10 io 1(
it (0 (0 to to 2
10 10 "0
10 /0 10 10 1
10 q
/0 10 10 3
to 10 1()
10 0
to (0
t 10 10
/
0 0
it 10 1(
to I0(
Ib I tO
( )
I
()
Io lo- /o{0 5
D 10 0 10 10 10 10 t0 /0 10 t0 10,c 6
11 i
10 11 ID 101 1 M 0to 10(
A = Pan weight (nag)
Tray color code:: "L"r.,!!
t OI9 Analyst:
W 1.* II6."1( 15.71Z5 1.5iV It.'jq 15.5% 1L.9S 15.Iq ISM I.0% 15A..
Date:
O.'tV B = Pan + Larvae weight (mg)
- ..j
[
Analyst:
- tu "L
ZL.'*
.'.202~z 2zk1o Zj1..4 2233 1
oso ZOSO Q9 zixi'12. ;2z.I.'*3 Date:
O0-2.1-M 421 C = Larvae weight (mg) = B - A Hand calculated.
to0 (o.01*
6,11 S.?2-SA.W60 1.TtA S S.6 IS-25 S-Ir 46,20 (01S Analyst:
.J Weight per initial number of larvae (mg)
C / Initial number of larvae Hand calculated.
Analyst:
0 C.,
V Q
0%-
4IV
'*9 Average weight per Percent reduction Initial number of from control (%)
0
- 61 larvae (mg)
I O~OO
-Wo.
7.
J Comment codes: c = clear, d = dead, fg = fungus, k = killed, in = missing, sk sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:.
I~
age 55 of 105 SOP AT20 - Exhibit.AT20.3, revision 07-01-12
PETS 0
Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date:
.A'04-"1-%N Survival and Growth Data Day 43.2%
86.4%
100%
M N
0 P
Q R
S T
U V
W X
0 1i.0 10 t1 to '
o to l o to Io 'tO to to 1
10(0 10 t
) 010 0 toI toO tO to 2
to I0 I
t to to I
o 10 (0 l0 IQ 10 to 3
I0 IO 10 10 o
to t
I to to to 4
1__
I0_/
0 10 1
'0
- 10)
I 1 (0 J.0 I1 I0
'I0 to 5
to-(
0 10 10 I0 t0 tIO"
()
to
,0 (O
6 10 I0 I0 It 10 IoC 16 1 10 to Ito to 7
2to t
.o to )10 t
o to 19 O 10 t 10 t
A = Pan weight Tray color code.. %X*,*qt)Qo%
Analyst:
WOO 1.7-- 5-3 65.25 6Io 15
.1015l.5* r.S* t51q Io 16.() 6%
1614 Date:
pg. tai
- B = Pan + Larvae weight (mg)
Analyst:
2A.
Date:
CA-.2.7- 0522.1 Z
1,5. Z.0D1 ZO..V ZI.53 ZoMIPV3.. ?.l 7.1.99 Z'M.I C = Larvae weight (nag) - B - A Hand calculated.
.66~ 6:11 s.941 S;3 sAO s.2R lo.ik S.-ioj;.ej Ib.q.1 j.(
Analyst:
% j -I Weight per initial number of larvae (mg)
Cn/dInitial number of larvae
)l, 0
Hand calculated.
4T AUR--S-Average weight per Percent reduction initial number of fromncontrol(%)
O.s'6
-tA6 1.
Ab 0,*2.
0.606 6A 7, larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w wounded.
Comments:
age 56 of 105 SOP AT20 -Exhibit AT20.3, revision 07-01-12
aETo Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date:
CA-r 1-1' i
Day 100% Intake
_ Y Z
AA BB 0
tA)
/D 1
10 1D 0I 2
/ (
10 ID) 3 t o.1 I
1 to 10 1 G to 10 0 1) 1t 6
10 It) i0) 10 A = Pan weight (mg)
Tray color code::
t__'v4tMb¢ Analyst:
16'0 14AS 15.05 j5 i Date:
. Sl B = Pan + Larvae weight (mg)
Analyst:
.. O.
Date:
091.l%
22Z 5
Z. 4q8Z1,(
C = Larvae weight (mg) = B - A Hand calculated.
&.1 t (
PAS 11111 Analyst.
Weight per Initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Ile Analyst:
Average weight per Percent reduction initial number of from control (%)
O, b*
-i".7.
larvae (mg)
I Comment codes: c = clear, d = dead, fg = fihgus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
age 57 of 105
.SOP AT20 - Exhibit AT20.3, revision 07-01-12
TSo SEnvironmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated September 17-24, 2013 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project numben 9261
_Not for Compliance Assessment, Interna] Laboratory QC Coeenmrtisou
(%)
8opflcate tnltt.Jdeiterof Fbit-0nnm erlu.t A -Pmnweight (im) 8-I'm.
cryoe Lanv.mtegbt(ais]
Wellhi/ Sarntun Meanwetgbtl Coe1ffiauiilenetldoa We~tllstttatll~I~mer Meamauan~ad Memwedghti ]it*ll Conlet*enof
]Peroontdetnloufr laraetwhltwet
-A -a onmberolt.,oae (a.
Sonvivtag nmba, f of nrlarem)
(is
%)
nanibela, thon vaination 0#.=
moe (it)
A 10 10 16.40 22.20 5.80 0.580 0.580 Control B
I0 1O 16.76 22.83 6.07 0.607 60.567 6.7 0.607 100.0
.567 6.7 Not applicable C
10 10 15.72 20.89 5.17 0.517 0.517 D
10 10 15.39 21.01 5.62 0.562 0.562 E
10 10 15.78 21.74 5.96 0.596 0.596 F
10
. 10 16.79 22.93 6.14 0.614 0.614 1000 0.600 4.8
-6.0 G
10 10 15.58 21.87 6.29 0.629 0
0.629 H
10 10 14.88 20.50 5.62 0.562 0.562 1
10 10 15.14 20.97 5.83 0.583 0.583 J
10 10 15.27 21.04 5.77 0.577 0.577 K
10 10 16.08 22.36 6.28 0.628 0.628 L
10 10 15.68 21.83 6.15 0.615 0.615 M
10 10 16.28 22.27 5.99 0.599 0.599 N
10 10 16.50 22.16 5.66 0.566 0.566 4
0 10 10 15.38 21.59 6.21 0.621 0.621 P
10 10 15.25 21.09 5.84 0.584 0.584 Q
10 10 14.70 20.08 5.38 0.538 0.538 R
10 10 15.63 21.53 5.90 0.590 0.590 S
10 10 15.18 20.47 5.29 0.529 0.5291 T
10 10 15.58 22.32 6.74 0.674 0.674 U
10 10 15.79 21.49 5.70 0.570 0.570 100%
V 10 10 16.01 21.99 5.98 0.598 0.606 5.3 0.598 100.0 0.606
- 5.
-&9 W
10 10 15.41 21.88 6.47 0.647 0.647 X
10 10 16.14 22.21 6.07 0.607 0.607 Y
10 10 16.05 22.23 6.18 0.618 0.618 Z0 9
14.48 20.59 6.11 0.679 0627 7.5 0.611 975 0.610 5.2
-7.6 10%ItaI.
AA 10 10 15.05 21.48 6.43 0.643 0.643 L
BB 10 10 15.41 21.08 5.67 0.567 0.567 Outranl 101:
Dunnett's MSD) value:
PMSD:
Intake:
Dunnett's MSD value-PMSD:
0.0655 11.6 0.0479 L.s MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of lest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effloent toxicity test.
Lower PMSD bound deteromined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined byUSEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Inteiabormlony Variability Study (USEPA, 2001a; USEPA, 2001 b).
USEPA. 2001a, 2001b. Final Repoit Inlerlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test 'Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: sqn101_091713data-uv.xtsx Entered by: J Sty~per Reviewed by:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated September 17-24, 2013 Statistical Analyses
- ETS Ib EnvjmnmQnta11hftSo1ut1onk1-Larval Fish Growth and Survival Test-7 Day Growth Start Date:
9/17/2013 Test ID:
PpFRCR Sample ID:
TVA/SQN101 End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
UV-treated Conc-%
1 2
3 4
D-Control 0.5800 0.6070 0,5170 0.5620 10.8 0.5960 0,6140 0.6290 0.5620 21.6 0.5830 0.5770 0.6280 0.6150 43.2 0.5990 0.5660 0.6210 0.5840 86.4 0.5380 0.5900 0.5290 0.6740 100 0.5700 0.5980 0.6470 0.6070 Intake 0.6180 0.6110 0.6430 0.5670 Transform: Untransformed Conc-%
Mean N-Mean Mean Min Max CV%
N D-Control 0.5665 1.0000 0.5665 0.5170 0.6070 6.678 4
10.8 0.6003 1.0596 0.6003 0.5620 0.6290 4.806 4
21.6 0.6008 1.0605 0,6008 0.5770 0.6280 4.105 4
43.2 0.5925 1.0459 0.5925 0.5560 0.6210 3.933 4
86.4 0.5828 1.0287 0.5828 0.5290 0.6740 11.413 4
100.0.6055 1.0688 0.6055 0.5700 0.6470 5.258 4
Intake 0.6098 1.0763 0.6098 0.5670 0.6430 5.189 4
1-Tailed Isotonic t-Stat Critical MSD Mean N-Mean 0,5914 1.0000
-1.243 2.410 0.0655 0.5914 1.0000
-1.261 2.410 0.0655 0.5914 1.0000
-0.957 2.410 0.0655 0.5914 1.0000
-0.598 2.410 0.0655 0.5914 1.0000
-1.436 2.410 0.0655 0.5914 1.0000 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01)
Bartlett's Test indicates equal variances (p = 0.45)
Hypothesis Test (1-tall, 0.05)
NOEC LOEC ChV Dunnett's Test 100
>100 Treatments vs D-Control Linear Inten Point SD 95% CL(Exp)
Skew IC05
>100 IC10
>100 IC15
>100 IC20
>100 IC25
>100 IC40
>100 IC50
>100 Statistic Critical Skew Kurt 0.96329 0.884 0.56553 0.80102 4.68917 15.0863 TU MSDu MSDp MSB MSE F-Prob df 1
0.06545 0.11554 0.00085 0.00148 0.71837 5, 18 polatlon (200 Resamples) 1.0 1 0.9-0.8:
0.7:
0,6-0.5:
0 04
'0.3 0.2-0.1 -
0.0 i.0 _!.-...... t.!
-0.1
-0.2 50 100 150 0
Dose %
Dose-Response Plot 1-tail, 0.05 level of significance (tage 59 of 105 File: sqnl0l091713data-uv.xsx Entered by. J. Sumner Reviewed by:__*
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated September 17-24, 2013 SET Environmontal lesting Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
9117/2013 Test ID: PpFRCR Sample ID:
TVA / SQN101 - Intake End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date: September 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
UV-treated Conc-%
1 2
3 4
D-Control 0.5800 0.6070 0.5170 0.5620 10.8 0.5960 0.6140 0.6290 0.5620 21.6 0.5830 0.5770 0.6280 0.6150 43.2 0.5990 0.5660 0.6210 0.5840 86.4 0.5380 0.5900 0.5290 0.6740 100 0.5700 0.5980 0.6470 0.6070 Intake 0.6180 0.6110 0.6430 0.5670 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 0.5665 1.0000 0.5665 0.5170 0.6070 6.678 4
10.8 0.6003 1.0596 0.6003 0.5620 0;6290 4.806 4
21.6 0.6008 1.0605 0.6008 0.5770 0.6280 4.105 4
43.2 0.5925 1.0459 0.5925 0.5660 0.6210 3.933 4
86.4 0.5828 1.0287 0.5828 0.5290 0.6740 11.413 4
100 0.6055 1.0688 0.6055 0.5700 0.6470 5.258 4
Intake 0.6098 1.0763 0.6098 0.5670 0.6430 5.189 4
-1.754 1.943 0.0479 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92263 0.749
-0.5281
-0.7039 F-Test indicates equal variances (p = 0.78) 1.42969 47.4683 Hypothesis Test (I-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.04791 0.08458 0.00374 0.00122 0.12996 1, 6 Treatments vs D-Control Dose-Response Plot 0.8 0.7 0.6 0.5 0 0.4 00.3 0.2 0.1 0
g tage.66Oof 105 1-tail, 0.05 level of significance OR II C1 oC1.-
o q-W e
File: sqnI0I_091713data-uv.xlsx Entered by: J. Surpmer Reviewed by:
+
PoETS
'--------=Page 5 of 6 Species: Pinephales promelas Client: TVA / Sequovah Nuclear Plant, Outfall 101, UV-treated Daily Chemistry:
Date:
AM-11'-1b Day (Analyst identified for each da, performed p-D.O. and conductivi measurements only.)
.Analysti
£,
Concentration Parameter pH (s.u.)
".q DO (mg/L)
I A '***
],
Conductivity CONTROL (Iirnhoslcm)
UV-treated
- Alkalinty t
(Mg CaCO3IL) 0
- Hardness (mg CaCO3/L),
ot"
- Temperature jC) 1-..
"*L'"
DH (S.U.)
o DO (mg/IL) 10.8%
Conductivity.
(jcmh Icm
- Temnerature (OC) 9 pH (S.U.)
DO (mg/M 21.6%
Conductivity (grnhos/cm)
I
- Temperature (0C) pH (S.U.)
DO (mgjL) 43.2%
Conductivity
(_Tmhos/cpr)
I *Temperature (C1C) flaq
-I.%-to 1480 GQ AC) 0 14.0 0 q.A15 A16 a
Z' lao lm.o.
'Iq ILS-0 f4jea
-I.%o q LIM)
To-9L 1-79 04 150 fro k9o Lo %
tft*
46 nH (S.U.)
86.4%
DO (mg/L )
Conductivt (iimhoscm)
- "~o
,1n *p oI11 r*toll%
pH (S.U.)
DO (mg/L) 100%
Conductivity
[
(gmrho/cm)
- Alkalinity
- Hardness
- TR.chlorln~e (mg/I,)
40aD
- Temperature (0C' "1c(.1 U,
'I ~
pH (S.U.)
DO (mg/L) 100%
Intake Conductivity (gcmhos/cm)
IG
-Alkalinity e2 (mg CaCO31L)
- Hardness (mg CaCO3/L)
- TR chlorine (mg/L) ~oi
-Ie I
"'L.UIII I Cemera-u I
4a LN.' F 4.*I.I II
- I Initial t "Final Initial Final Initial 11 Final
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
inity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
_SOP AT20 - Exhibit AT20.3, revision 07-01-12 age 61 of 105
'ETS Page 6 of 6 Species: Pimephales promelas Client: TVA / Sequovah Nuclear Plant, Outfall 101. UV-treated Date:
ot-ri\\'
I Day (Analyst identified for each day, performed pI-, D.O. and conductivity measurements only.)
I 3
I 4
I 5
I 6
Analyst Concen-Parameter tration I
DH (S.U.)
CONTROL UV-treated DO mg&
PCI Conductivity Z
(wig CaCO3IL)
- Hardness (wgCaC0 3/L)
- Temnerature 0C)
IPH (S.U.)
DO (mg/L) 10.8%
Conductivity (Ta*hos/cm)
_________*Temperature
(*Q- )
DH (S.U.)
m q
I 2
" m II DO (mg/L) 21.6%
Conductivity Oumhos/cm)
- Temnerature (°'O
- Tepertur C
pH (S.U.)
DO (mRwL' 43.2%
Conductivity (umhos/cm)
- Temperature (0C)
PH (S.U.)
__'_(O--
DO (mg/L)
-)
86.4%
Conductivity
_T__em r
-62.O "L91_9 Temperature C0C)
'LS.0-A 61M
-0 4sga WS-S.O)
W
.IS0 1
i1 pH (S.U.)
'3 -to I
U -~-~--
It ~2~~-'-~-.-
I -~----q:
(~ -'*~--
- ~
DO (mg/L) i*, an I
100%
Conductivity (gmhlos/cm)
- Alkalinity (mg CaCO3/L)
- Hardness
- (mig CaCO3IL)
- TR chlorine (mag/L)
- Temperature (OC)
('1
~0. ID Is' I
U F
~
DIH (S.U.)
I 7qS 11 l'4.Zt I
- .,A II I-iDO (mg/L) 100%
Intake Conductivity mtuhos/cm)
- Alkalinity (Mg CaCO3/L)
- Hardness (mg CaC0 3IL)
- TR chlorine (mg/L).
- Temperature (00)
Initial.
Final Final I
!f.gmperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page I.
- kIislity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
---j age 62o 1Of 5
.SOP AT20 - Exhibit AT20.3, revision 07-01-12 itage62 of 10
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated September 17-24, 2013 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 O E T Daily Chemical Analyses O
Environmental Testing Solutions, Inc.
01 Project number:
9261 Concentration Parameter Dao 0 Day 1 Da 2 Day,3 Day 4 Day 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final PH (SU) 7.89 7.72 7.85 7.46 7.81 7.65 7.93 7.51 7.95 7.68 7.99 7.68 8.01 7.66 DO (mg/L) 7.9 8.2 8.3 7.0 7.8 7.5 7.9 6.7 8.0 7.3 8.0 7.2-7.8 7.4 Conductivity (pmhos/em) 304 307,
308 '
303 305 SW 305 j
311 fU8 Control Alkalinity (mg/L CaCO3) 60 100"*6 Q '
60 S Th.
R!
Hardness (mg/L CaCO3) 90 84 *Ia w
90 fax 0
.11.
O Temperature (*C) 24.9
.25.0 24.8 24.6 24.9 24.5 24.8 24.8 24.8 24.6 24.9 24.7 24.8 24.6 pH (SU) 7.89 7.71 7.86 7.41 7.80 7.66 7.94 7.58 7.96 7.68 7.96 7.69 8.00 7.62 10.8%
DO (mg/L) 7.9 8.2 8.3 7.0 7.9 7.5 7.9 6.8 7.9 7.3 8.0 7.4 7.8 7.4 Conductivity (pmhos/em) 290
,S.
295 297 288 2
295
,i 296
- 2.
283 I Temperature (OC) 25.0 24.9 24.9 24.5 25.0 24.7 24.9 24.7 24.9 24.3 25.0 24.6 24.9 24.7 pH (SU) 7.88 7.71 7.86 7.42 7.77 7.69 7.94 7.53 7.97 7.67 7.97 7.71 8.01 7.67 DO (maLL) 8.0
- 8. 1 8.3 6.6 8.0 7.5 8.0 7.0 7.9 7.3 8.0 7.5 7.8 7.4 21.6%
Conductivity (ljmbos/cm) 276 tui.P 278
'§e -
280 273
- -,r,-K.,
279 2 280 W 276 Temperature ('C) 25.0 24.9 24.9 24.5 25.0 24.6 24.9 24.7 24.9 24.3 25.1 24.8 25.0 24.7 PH (SU) 7.85 7.71 7.86 7.41 7.72 7.64 7.93 7.49 7.96 7.63 7.99 7.68 8.00 7.66 43.2%
DO (mg/L) 8.0 8.1 8.3 6.6 8.0 7.5 8.0 7.0 7.9 7.4 8.0 7.4 7.9 7.4 Conductivity (gmlaos/em) 245 245 248
- 242 247 g, 247 247 T
Temperature (°C) 25.1 24.8 25.0 24.8 25.0 24.6 24.9 24.8 25.0 24.2 25.1 24.5 25.0 24.5 PH (SU) 7.81 7.71 7.86 7.43 7.63 7.69 7.86 7.45 7.92 7.66 7.96 7.68 7.97 7.60 DO (mg/L) 8.1 8.1 8.4 6.5 8.1 7.5 8.0 6.6 7.9 7.4 8.0 7.3 8.0 7.5 86.4%
Conductivity (pmhos/em) 181 179 181 t
178.
183 179 182 i
ý Temperature (7C) 25.1 24.9 25.0 24.7 25.1 24.6 25.0 24.6 25.0 24.5 25.1 24.5 25.0 24.8 PH (SU) 7.76 7.67 7.83 7.37 7.55 7.59 7.83 7.46 7.90 7.62 7.97 7.60 7.95 7.61 DO (mg/L) 8.2 8.1 8.4 6.7 8.2 7.5 8.0 6.6 7.9 7.4 8.0 7.3 8.0 7.5 Conductivity (gmhos/cm) 158 3W;
-. t' 160 r..
153 154 1,4,
. 157 157.,
A 158 Q, 100%
Alkalinity (mg/L CaCO,)
60.
61WO
- 0.
,1 61 4i__f ON
ý I*
ON__
61 N=61 0
Hardness nglL CaCO3 )
66 2.7 6N'
._N 66t 68 KI-IWO.
- Total Residual Chlorine (malL)
<0. 0
<0.I0 7
V-
<0.10 Temperature (CQ 25.2 24.9 25.1 24.7 25.2 24.7 25.0 24.6 25.1 24.4 25.2 24.5 25.0 24.8 PH (SU) 7.71 7.70 7.81 7.47 7.54 7.67 7.83 47A3 7.88 7.63 7.90 7.75.
7.95 7.69 DO (magL) 8.2 8.1 8.4 6.8 8.3 7.5 8.0 6.6 8.0 7.4 8.0 7.3 8.0 7.5-Conductivity (jemhos/cm) 156 156 156 4 150 P 156 157 trtl 155' 100%Intake Alkalinity(mg/LCaCO3 )
62 6
61 T. M-fN Hardness (mg1L CaCO3) 64_~..
Y 70
- Total Residual Chlorine (mg/L)
<0.10
- nO
'*N1-.
0
<0.10 tl
- .U cO.10 In Temperature (C) 25.1 24.9 25.0 24.7 25.1 24.7 25.0 24.6 25.0 24.4 25.0 24.7 25.1 24.8
- Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
File: sqn101_091713chem-UV.xls Entered by: S. Tpylr Reviewed by:
Iic I*..
I I
II
".:I I!.
S S
0 0
EnvIonn.enblTestlng Solutions, Inc.
Page Page
. (_of q_
Time initiated LA Time completed 13DI Analyst I P Date analyzed 1094Y4jj3 Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Titrant normalitv and multiviler determination:
pH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2CO 3 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml ml ml
= 0.25/E
= N x 500
=4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220) 6,* IlO~e~o
- 0.()y 0.* k
- 7
_-.0.o*
,, /o' I
Laborat I g at ooo.
.-o, Reference standard True value Sample Alkalinity(MV)
%RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (Iag CaCO3/L)
(acceptable range (Mg CaCOAL)
(ml) ml ml ml f 90 to 110%)
t4*l'tS 100 1oo
/AX*
A*1.2-0.0 O..
02,,-,*O/
Duplicate sam le precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)
{(S - D) /[(S+D)/21) x 100 number
,_ml_
ml ml ml (acceptable range =: 10%)
)q.1ca.1
$SSW
/00.4~
3.7-10 s
3 Matrix spike reeove*
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (ag CaCO3IL)
(rag CaCO3/L)
(ml) ml ml ml Ia, 'SIqt
°° q.4l SZ I)'V J6 11 Sample alkalinity (B)
Measured spike value (MV)
% R MV /SV x 100 (mg CaCO 3 L)
MV = A - B (acceptable range (Mg CaCOAL)
= 75 to 125%)
33 45 qo~o/.
Sample measurements:
Sample volume Begin
-End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mng CaCO3/L)
________10 (f$+20Y 3Z 60 t3 o I. 0 1,0-2 At 3&o fl4.O
- ,o t I o*,I.J:
10o
.o q..5 Q,.6 1 P
=
+/-1 11*-
_ _9-
.1 q.0 9
0__
.04P is~ is*
A.
o9.000.ls 5 tUL) 18
__z.0p Uoc 5.4 Ill
_.__0,__,__
36.0 4q... (M_______
Reviewed by:
Date reviewed: I &j. iq",
Page 64 of 105 SOP C6 - Exhibit C6.1. revision 01-01-13
- ES Envirwntment~ieung Solutionrý Inc.
Page 7W Page 2-- of _1 Analyste _
Date analyzed oq--]q,i"b Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3IL Titrate samples to pH = 4.5 S.U.
Time initiated V*-ý Time completed Titrant normality and multiplier determination:
PH Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check
-1egin
-f--
-Xo (5 ml Na2 CO3 x 0,05)/E
= (Nx 50000)/100 ml sample water reference standard ml ml,
ml
.25/E
= Nx 500
= 4.5 S.U.
number number
_E.
(acceptable range =
9g4.00,.
-'4 Laboratorp control standard.
Reference standard True value Sample Alkalinity (MV)
% RS =MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCOs/L)
(ml) ml ml ml 90 to 110%)
I hV561*
lOO VO 100 0.t 6
o Q q-o 10-z-q 2_
q;L'O/.
Duplicate sam Ple precision:
Sample Alkalinity
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mg CaCOSIL)
((S - D) /[(S+D)a1l) x 100 number (ml) ml rml ml (acceptable range =:L 10%)
.100 qV 1,5.1 4_0 10-__
S_
_T Duplicate (B)
D
_.D
,1 Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mng CaCOs/L)
(mg CaCO3/L)
(ml) ml ml ml l S~tIq,-,
e"7-.
100 3,0 2
'0
/o.q Z
Sample alkalinity (B)
Measured spike value (MV),
% R = MV /SV x 100 (rag CaCOs/L)
MV = A - B (acceptable range (mag CaCO3L)
- 75 to 125%)
Sample measurements:.
'Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3 /L) mam,1_.
_I.
.' J?
,o P3 sl.o 9,_-_*_
_________________0_1 1_
t-0
- 38.
j5q
.q
___10_
1___ 0, I_3_lq.
0-3 I1A3..f3
.3%
Reviewed by:
[ F I"]
Date reviewed: I oq-A14 Page 65 of 105 SOP C6 - Exhibit C6.1. revision 01-0 1-13
"ETS SEnviron'mental Testr,* 5olutionrmIn*
Page %e Page of of Analyst U
Date analyzed LiItI Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time initiated I'-,
Time completed 1, a q IZ Laboratorv control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume.
Begin End Total Multiplier (mg CaCO3IL)
(acceptable range (mng CaCO3JL)
(ml) ml ml ml
= 90 to 110%)
I j0(0*5I3*2o 100 13.5 2*5.
0
/oIz.
Duplicate sam ple precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
{(S -D) /[(S+D)/2]} x 100 number (ml) ml ml ml (acceptable range =-- 10%)
%,0At0%F.lA\\
zW 2
/.
A.
.g I
l s
42
.1 Duplicate (B) 444 D_
142-_
Matrix spike reco Reference standard Spike value Sample oqli1l Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mag CaCO3IL)
(mg CaCO3/L)
(ml) ml ml ml Sample alkalinity (B)
Measured spike value (MV)
% R =MV /SVx 100 (rag CaCO3/L)
MV = A - B (acceptable range (Mg CaCOAL) fi75 to 125%)
4/2 oo-I.
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample iD (mi) ml ml ml Multiplier (ma CaCOa/L)
F wj 3
160 35a A.-
__04 qo
-b A Vi MJ 6,2-.
5 1
0L 3
5.2 1S4.3 J'I 160
- I
.+/-1.
"A I o*L ilOt I0,U 13.q 2.8 1'600-A
'k 13.'
?q 19 Ilos o.Os CjV&o U),n 1
2__
013.0 1
.1 5.
(
_Z;_
29__
z, 1 5,
223.
Reviewed by: LI XIJ Date reviewed: I a q,"14 '.3 Page 66 of 105 SOP C6 -Exhibit C6.1. revision 01-01-13
ET Envlmnmfentnl Testing SnutionsInc.
Page
__7 Page q of Analyst I
Date analyzed [0q.jq4 I
Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time initiated Time completed Z Z J Titrant normalitv and multiplier determination:
L Normality Normality (N) of H 2SO 4 pH Factor or Multiplier Deionized el r..t
-Begin End Total
= (5 ml Na 2CO3 x 0.05)/E
= (N x 50000)/100 ml sample water reference standard ml
=
0.25/E N x 500 4.5 S.U.
number number (E)
______-_-_L LI.01_0)
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL)
(acceptable range (mg CaCO3/L)
(mI) ml ml ml
= 90 to 110%)
Duplicate sam pie precision:
Sample Alkalinity
%RPD.=
Sample Sample ID.
volume Begin End Total Multiplier (mg CaCO3/L)
{(S - D) /[(S+D)/2]) x 100 number
_(In) ml ml ml (acceptable range = : 10%)
h____.'l,_
SSW_
/00 /33 I(.
2 s
3 I, Duplicate (B)
I IN,'
1q1-,
31 D.1 4,32.
1_
3_._.
Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mag CaCOIL)
(mg CaCO3l)
(ml) ml ml ml
__00__
kIL)
AU J'p 10,2 Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mag CaC~h/L)
MV =A -B (acceptable range (Ing CaCO3/L)
= 75 to 125%)
32 qp 0.of/.
Sample measurements.
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOsiL)
,5 01.06.o 1
b_
_0.2 lbo.t ".4. -0 6.
3o1 0-Reviewed by:
[ZI.II]
Date reviewed:
Page 67 of 105 SOP C6 - Exhibit C6. 1. revision 01-01-13
W" eES 6
EnvifonmentalTesttng Salutlons, Inc.
Page Page I of f Time initiated
/0 Time completed
[.
Analyst 7V-Date analyzed L,
I Th~eant normality and mnulfinliPe dplprmnuanunn_.
Alkalinity (SM 2320 B-1997) 4atrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.5 S.U.
__l_
__o_
pH of Normality Normality (N) of H2S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml Na 2CO3 x 0.05)/E (N x 50000)/100 ml sample water reference standard ml ml ml
= '.25/E
= N x 500 4.5 S.U.
number number (E).. (acceptable range = 0.0180 - 0.0220) q~
I*w so IsqU'i ab 12.4 0.0022-.
0.1 Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
%RS=MV/TVxIOO number (TV) volume Begin End Total Multiplier (mg CaCO31L)
(acceptable range (mg CaCOs/L)
(ml) ml ml ml
=90 to 110%)
bt tq V100 100 11a.q Io/0/
Duplicate sam ple precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCOA/L)
I(S - D) /[(S+D)t2]) x 100 number (ml) ml ml ml (acceptable range =:L 10%)
Duplicate (B)
D (eo 4
Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(rag CaCO3/L)
(ml)
Ml ml ml Sample alkalinity (B)
Measured spike value (MV)
% R = MV /SVx 100 (mg CaCO3/L)
MV = A - B (acceptable range (mg CaCOa/L) 75 to 125%)
- 1.
q1 1411 Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Mng CaCOA/L) 6111,13__
1fLS~
60 A&i 1qq-6A 101 01A,1 11 0.0 4 A_
_0 o11,-A.,U
.. 6 1 38 a.s
\\0
_3
%V 1380 141.60 1 _.__
i
,0 ge=
'.i 1o Reviewed by:
Date reviewed: I Oct. 2i1 Page 68 of 105 SOP C6 - Exhibit C6. 1. revision 01-01-13
4 nvimronmental Tieting Solutions, Inc.
Page _t Analyst I P, Date analyzed Lq,,i I" !
IZ Titrant normalitv and multinlier determinatlos, Alkalinity (SM 2320 B-1997)
Matrix: Water, RL = 1.0 mng CaCO3/L Titrate samples to pH = 4.5 S.U.
Page
._L of Time initiated Time completed pH of Norimaity1f3 Normality (A) of H 2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Totsa I a0 3 x
ý005)/E
= (N x 50000)/ 100 ml sample water reference standard ml ml Mil
=.
Nx500
=4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (nag CaCO3/L)
(acceptable range (mag CaCO3L)
(ml)
M I mi ml
=90 to 110%)
[R g5e lq to 100 100 6.0 qb 9.0 "6.o Duplicate sam pie precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mag CaCOA/L)
{(S - D) /[(S+D)t2]} x 100 number (ml) ml i
ml (acceptable range =.+ 10°%)
V11001 UnI_ lyS1 Tr lo9 VO 1.!
t.o IO0I
- 9.
iv t 0
/0-1 Rfn Duplicate (B) iso 2.0 110.0 D
Matrix spike recovery:
- Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Mg CaCOsIL)
(m1 CaCO3/L)
(ml) mi mi MI tdNI OZ-,s ISID 96(,5o 03-14t,1
/10 Sample alkalinity (B)
Measured spike value (MV)
%R=MV/SVx10 (mg CaCO3/L)
MV = A-B (acceptable range (Mg CaCO3/L)
= 75 to 125%)
Sample measurements:
- Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml mi Multiplier (rag CaCOsL)
$ih CA2b
- t.
%L
. 3 3is Gii-01M..oI 1VA.5 lOiWJ '
3,'
43.3 Lb t4,3
~bcas~.~0.610
,.o 0~.0.
60_
1 61 AV 3"_ 1(o 40 Ub9.
al 2.00
___i
%&t~l. it Cen CD2..
_ZG Q'. 4 -1Leoq S.,
(4)
Z.30 1-606,14.1l O
UDW07
_ 23....
i)
-ww v
v Reviewed by:
V 'I Date reviewed: I OCI'10 Page 69 of 105 SOP C6 - Exhibit C6.1. revision 01-01-13
Env~~ ~
~~lrmt uletn Sdtoris. Inc.
Page 22 Page I
Of____
Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO 3/L Analyst [K; Date analyzed Odi~Iii]
Titrant normality and multilier determination:
Time initiated [
13( al
[
Time completed LiAM Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E (N x 50000)/ 50 ml sample number number (E)
(acceptable range = 0.0180 - 0.0220)
Nx 1000 1£q A "n 5I lze 0.0 10.0
/0.0 O..2-OO0 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range I
(Mg CaCO3/L)
(ml) ml ml ml
- 90 to 110%)
I N',Sli'4 z.-
40 j5o0 H_,___,____,______O___
Duplicate sam le precision:
Sample Hardness
%RPD=
Sample Sample ID volume Begin End Total Multiplier (rag CaCO3IL)
((S - D) /[(S+D)/21) x 100 number (ml) ml ml ml 0q.1o. 13
- ,St, 6"
12.0 I/.
Z-1 So s q2 jDuplicate(B)
J.
I 1..z, D
q4.
07e'l3 Matrix spike recoveri:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3IL)
(ml) ml ml ml 140 tD Vqj
/ &zO 4,1 Sample hardness (B)
Measured spike value (MV)
% R = MV/ SV x100 (rag CaCO3/L)
MV = A - B (acceptable range (mg CaCO3/L) 75 to 125%)
- 2.
q/o
- /oto'/.
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV =ND BankbCC~
- 50.
0.0 0,t) 0'0
~
d P-
= *(should be = 0 mg CaCO3I.,)
0 '0
- 09.
q-13
- 5604
/__
_8e-2 ada.z
.20oD IaI6,01-J/
o0.2______
2.0o
___1.1 p 14.
4.3 156
____j:
203tim'
+10' 9z~
o i 10- i31
___.1_
3a.1 0.1-s 90 0,1-o,_I.
/3__0, 1 40.2
.,k' qz l'bo-___..5
_A___-__L 4.2Q.. 5 7,i'3 g
Lt?
z I Z.5 Note: If >1 5ml of titrant is used, sample must be diluted.
Reviewed by: Ll4 lill i Date reviewed i3O-llJ SOP C7 - Exhibit C7.1. revision 01-01-13 Page 70 of 105
"ETS 6
Environmental Testing Solutions, Inc.
Page 23 Page Z of Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3/L Analyst
[IL*,777II]
Date analyzed bqiiA-v
]
Time initiated Time completed[
I ZI Tltrant normality and multiplier determination:
Laboratory control standard:
Reference standard True value Sample Hardness (MV)
%RS=MV/TV x100 number (TV) volume Begin End Total Multiplier (rag CaCOa/L)
(acceptable range (111139 CCO3/L)
(MI) ml ml
= 90 to 110%)
.40 50 qj. G Z.4 I
1.00
- 42.
Duplicate sam le precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L)
((S -D) /[(S+D)/21) x 100 number 7 (ml) ml ml ml.
,ejl,0.o'4 fthils I b0.
O
..oQ.
in
'o s Ito 0 Duplicate (B)
-0 D
j 40-s
-~..hm 24-e 3.11,4 0j01 Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number' (SV) volume Begin End Total Multiplier (ag CaCOsIL)
(Ing CaCO3,)
(ml) ml ml ml o~'
Z 1.0 Sample hardness (B)
Measured spike value (MV)
% R = MV /SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (Ing CaCO3/L)
=75 to 125%)
loo 0
Wiadi.
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mi CaCO3 L)
Blak
_-Ba
.TV__-___
-( should be= 0 mg CaCO3IL).)
moI1M.O4 MMPtI I14.4 45 (Z) 20.0 180.
N-0q
- L g7 I
.5 ?
.5 q.1 1.
l00
_6_
95 i
?.1.o z1.
'1.3 I,
Z 1-61AII-' 74I 217.1 i
1.3
.3-I1 Ifn 1.21 3od I 2*.- ts 1.7-3.
-. 5 3Z2.Z_
332Z 3?F W
11C) f4VA ton Cin UU;1 Note: If >15mi of titrant is used, sample must be diluted.
Reviewed by: f i/ii I
Date reviewed o
A Page 71 of 105 SOP C7 -Exhibit C7.1. revision 01-01-13
@ST ErI omnaletn Seulons, Inc.
Page __q Page.~
of Hardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3/L Analyst [
U.
Date analyzed oi4-Njo j Time initiated Time completed Titrant normait and multiplier determination:
I T -as
-Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference Vstandar
= 0.2/E
(N x 50000)/50 ml sample number number (EN)(acceptablran
1000 I
Laboratory control standard:
Reference standard True value Sample Hardness (MV)
%RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO3IL)
(ml) ml ml ml
= 90 to 110%)
45S~J~
Ikl4 0
- 4.
.10 ?.O 2010)
McL)
Ia 7
Duplicate sam le precision:
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
{(S - D) /[(S+D)i2]) x 100 number (ml) nml ml mln M,.. L 2-12-5.34O 20.0 s
-0 Duplicate (B) iorf
" 4 D
"IO'~l3 Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Mrg CaCO3/L)
(rgCaCO/L,)
3ml ml m
ml
)l!SSl107, 2T 1--
.0 14-.1 31o Sample hardness (B)
Measured spike value (MV)
% R = MV/ SVx 100 (mg CaCO3/L)
MV = A -B (acceptable range (mg CaCO/L) 75 to 125%)
1-3o 0
- D0o*
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV -J]D Blank 2o:gq-4.=- X1 'j-,4-'+ (7) b
-l0 mcio~to--L.
" A CC 003 10 30,.O P
?q-11. )
_6_
1 11
_____I IPT 5 j 31A' 045S Z
160 Note: If >1 5ml of titrant is used, sample must be diluted. ' Reviewed by: Ij j
j j
Date reviewed I a*-
.A-.
Page 72 of 105 SOP C7 - Exhibit C7.1. revision 01-01-13
I~.
I.
I I
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eES a EnvironmentalITesting Solutlonis, lnc.
Page As Hardness (SM 2340 C-1997)
RL =1.0 mg CaCO3IL AnaystIO Date analyzed Titrant normality and multlnlier determination:
Page __._
of 2..
Time initiated Time completed
]
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E
= (N x 50000)/50 mil sample number number (E)
(acceptable range = 0.0180 -0.0220)
= N x 1000 IMPS-5-,
I Ha*rSdness 0.0
%R I0,0SM 0,/xo10 Laboratory control standard."
Reference standard True value Sample Hardness (MV)
%RS=MV/TVx 10 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mag CaCO3Ll) ml ml ml I
90 to 110%)
-Iq**l*..
40 50 LIZ/,I z,
2,,!*t/
Duplicate sam ple precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)
{(S - D) /[(S+D)/2]) x 100 number (ml) ml ml ml Duplicate (B)
J 1I 4
D go-.~~z, Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mag CaCO/L)
(ml) ml ml ml st~
4
/30i
- h.
u.o 130 Sample hardness (B)
Measured spike value (MV)
% R= MV /SVx 100 (mg CaCO3/L)
MV = A - B (acceptable range (mg CaCO3/L)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (ma CaCO3/L)
TV ND Blank TV =ND (should be = 0 mg CaCO3/L)
£51) 0,0
- 00. 0-Z-00 (Y~f.LLA) 9-4.
.3.
&IA6
's.
I 6A fl.0 14.$
___9 o%1.,.
V11 3&s oO'*
q.,*q__
3.*.*1 0
.I,4 f.Z.
_4 I,,.L I
-&CA*
00.0 60,?*;1 69 o0
~~~~~~~~~~~ 0p0-.
L
.)
i,.
0')
lb i.
A~~c 1j3,3
%So%%
q&
Q 3.4 Note: If >15ml of titrant is used, sample must be diluted.
Reviewed by: I Date reviewed I tii..t%-I1 Page 73 of 105 SOP C7 - Exhibit C7.1. revision 01-01-13
EETS Enironenta Teeng Sluttons, Inc.
Page Z(a Page 2-of 2 I
Analyst I/-
Date analyzed [0i, iaII
]
T2trant normality and multiplier determination:
[lardness (SM 2340 C-1997)
RL = 1.0 mg CaCO3/L Time initiated 00174 t3 Time completed U
U I
4n mality-check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard m
ml
-0.2/E (N x 50000)/50 ml sample number number table range = 0.0180 - 0.0220)
= Nx 1000 I
Laboratorp control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV/TV x 100I number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mn CaCO3 /L)
(roB ml ml ml
= 90 to 110%)
I
- 107, 40 50..
.0 As
- ,o
- Zo.0
'40 I0U).-
Duplicate sam ple precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End
-Total Multiplier (mg CaCOsIL)
{(S -D) /i(S+D)/2J} x 100 number (ml) ml nml ml
~~~~~6 1VA Q-50 A
O &.Z 32
-S
~~
Duplicate (B)
J, g6 I 3).
3.2-1 1 D..
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID L(ml) ml ml mil Multiplier (mg CaCOAL) rv.
B11~
T Zb (should be =0 Ing CaCO3U)
Vbo~~
-01 t
fA&)
0w a
S~~2.-__
?(P.5 to 4[_
IbA1.L.
3q (0_
1_q-Vb~*.'.
"CAI,&,
~lO
-%ýS 3*
q
(.
330 o
." 3 1o.1 3.q
%&o¶ Op. QL_ VA thvgT(Aj I lo,1 1.3.5 3.Z
%6 0q_ %
3.1 7
05____0 r_
%tv 3
6,5__
J go*,o 3*-ý.__I___L Note: If >15ml of titrant is used, sample must be diluted.
Reviewed by: [i
,i.II Page 74 of 105 Date reviewed SOP C7 - Exhibit C7.1. revision 01-01-13
Page 53 Page 1 of I S*T-(0 Environnental Testing Solutions, Inr.
Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed [
I" I Iodide reagent: XNR"5 Acidreagent: "7.
II s%5 Calibraion:
Reference standard number IWOf 1.th Ip mtbeitedZ thlq.
e
- Note: For samples with a residual chlorine of > 1.0 rag/L, the samples must be diluted to be within the calibration range.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number -.
(mg/L)
(mg/L)
(acceptable range = 90 to 110%)
0.50
/.
62_
,OLj.q 7.
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = [(S - D) /[(S+D)/2]) x 100 number I
(mg/L)
(acceptablerange = L10%)
I Duplicate D,
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number I
, (m r.*)
n bReagent Blank
_-__ -. Ctq_ _
I3q%0 Jiý O ho-2
_V )
A Q~
so
~-W no t*ey-d mvi-OLO-0O3(0'i Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Lboratory control standard.
SReference standard True value (TV)
Measured value (MV)
% RS MV/TVx 100 number I(aW/L)
I(mg/L)
(acceptable range= =90 to 110%)
SS It'aL 0.50
- 0. Li75 q,5 I-SReviewed by Date reviewed SOP C8 - Exhibit C8.1, revision 01-01-13 Page 75 of 105
rof Page I
of2 Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed [1I" Ii
,-I1 I
Iodide reagent: ývknks%?
Acid reagent: I z/fa, Gil 0.1Note:
Ft h0 mS/L teml m
l ioe Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 number (mg/L)
-(rg/L)
(acceptable range = 90 to 110%)
PJ*
u%,z o0.50 O.
ol.8.
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = {(S - D) /[(S+D)/2]) x 100 number
[
(aceptable range =:k 10%)
______t~
ND~tCa1or 0-kpu S 40'0 Duplicate
_De_
CC___
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number I
me/L)
Reagent Blank N.Jt~
sw eme-r po"C hc\\CAV&
ws
/1 0 O-W 1 *,*.A..
A*.seA,,,t,.-
- (.O jd G.e c
la,,
'eovdg we-*X-es /.O.OQx"2.\\
Note:. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratorn control standard:
Reference standard True value (TV) rMeasured value (MV)
%uRS = MV / TV x 100 (mg/L)
.(mg/L)
(acceptable range = 90 to 110%)
0.5 OA 0 -t.%, I Reviewed byI I
Date reviewed I ak 8 SOP C8 -Exhibit C8.1, revision 01-01-13 Page 76 of 105
Page 3s Page 2-of jentalTesting Solutlonsý Inc.
Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0. l0 mg/L Meter: Accunet Model AR25 pH/Ion Meter Analyst t.[
Date analyzed o[Q I
j Calibration:
Iodide reagent: I Acid reagent:
0.10 mg/L 1.00 ms/L 7
Slope I
.1 h
l I D]fmrnce standard number I
1:009-11k-15 Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range.
Laboratory control standard:
I I I
.Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number.
(rag/L)
(mg/L)
(acceptable range = 90 to 110%)
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = ((S - D) /[(S+D)/2]) x 100 number (m
(acceptable r'ange = *10%)
S 4.t-I 0-M-n l
Duplicate D <.00N9 iCLAR-1%
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number migt!!
Reagent Blank
.V.A WI V*
o l.%Orxo c6oo,-
CIW,r-
_,o.004
~~
EýW. co-L Ao cdov=
Clew-noAg,'
a
~
O cr~v-CýeUot-e QV no cotoe-dcr
_____C___
Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS MV /TV x 100 number (mrg/L)
I(mg/L)
(acceptable range = 90 to 110%)
0.50 A
.4-"*
It 4. to '.
Reviewed byI
- :o Date reviewed I
IOI. J SOP C8 -Exhibit C8.1, revision 01-01-13 Page 77 of 105
Page L
ofl i
SEnTS Q
Environmental Testing Solutions, Inc.
Total Residual Chlorine (ORION-1977)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst V6 Date analyzed 0[ ZI.1,-5 I.odide reagent:
lj7*j1 J
Acid reagent: L3 II Calibration:
0.10 mg/L the mg/L tlon rg Reference standard number.
1951161:I t*..ll
.1.0 lp Note: For samples with: a residual chlorine of > 1.0 rag1L, the samples must be diluted to be within the calibration range.
Duplicate sample Precision:
Sample Sample ID Sample characteristics
' Residual chlorine
%RPD = {(S - D))/[(S+D)/2l} x 100 number m(cetberne+1%
DulficateDrqW Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number 1411m/L)
Reagent Blank
(.0O02 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS =MV TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
I.
1S ( -
0.50 o,.Q 1
/
Reviewed by 1 t"
91 Date reviewed SOP C8 -Exhibit C8.1, revision 01-01-13 Page 78 of 105
Sequoyah Nuclear Plant Biomonitoring September 17 - 24, 2013 Appendix D Reference Toxicant Test and Control Chart Page 79 of 105
Environmental Testing Solutions, Inc.
Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.
I.
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I 0.9 H-I I
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I II USEPA Control Limits (+/- 2 Standard Deviations)
'",.................... K 0.8 0.7 0.6 0.5 I-I I
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1.4 1.2 1.0 0.8 N
L-2 0.6
- =
0.4 1.4 1.2 I
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I USEPA Warning and Control Limits (75th and 90th Percentile CVs)
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I Laboratory Warning and Control Limits (10th and 25th Percentile CVs)
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0.8 0.6 0.4 Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC2 5 )
Warning Limits (mean IC2 5 +/- SA.10 or SA.75)
Graphs generated from associated excel spreadsheet.
Control Limits (mean IC25 +/- SA+ 2, SA.90, or 2 Standard Deviations)
Reviepwnedahsafter..
J.sbmye:
~,
,*~,Reviewed by:
80 of 105
,w 0
Environmental Testing Solutlons, Inc.
(31 Test number Test date 7-day IC2 Cr (g/L KC)
L KCI)
Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory S
Control Limits SAM Warning Umits CT-2S Cr+2S Cr-sA.
Cr+SA, Laboratory 5AM Control ULmits Cr - SAM Cl+ S's USEPA SAM Warning Umits CT -S.M CT + SA.s USEPA SAMJ Control Limits Cr-SAM CCT+S CV 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 08-07-12 08-14-12 09-11-12 10-02-12 10-1&-12 11-0&-12 12-04-12 01-08-13 01-15-13 02-05-13 03-03-13 03-05-13 04-02-13 05-07-13 05-14-13 06-11-13 07-09-13 08-06-13 09-10-13 09-17-13 0.70 0.81 0.82 0.66 0.71 0.65.
0.77 0.74 0.76 0.65 0.74 0.79 0.77 0.63 0.78 0.73 0.74 0.68 0.83 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.73 0.74 0.73 0.73 0.73 0.73 0.73 0.73 0.73 0.05 0.62 0.81 0.05 0.63 0.82 0.05 0.64 0.83 0.05 0.64 0.83 0.05 0.64 0.83 0.05 0.62 0.83 0.05 0.63 0.83 0.05 0.63 0.83 0.05 0.63 0.83 0.05 0.62 0.83 0.05 0.62 0.83 0.05 0.63 0.83 0.05 0.64 0.83 0.05 0.62 0.84 0.06 0.61 0.85 0.05 0.63 0.84 0.05 0.63 0.84 0.05 0.62 0.84 0.06 0.62 0.85 0.06 0.61 0.85 0.09 0.63 0.09 0.64 0.09 0.65 0.09 0.65 0.09 0.65 0.09 0.64 0.09 0.64 0.09 064 0.09 0.64 0.09 0.64 0.09 0.64 0.09 0.64 0.09 0.65 0.09 0.64 0.09 0.64 0.09 0.63 0.09 0.65 0.09 0.64 0.09 0.65 0.09 0.64 0.80 0.81 022 0.82 0Z2 0-82 0.82 0.82 0.82 0.81 0.81 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.82 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.57 0.57 0.58 0.58 0.58 0.57 0.58 0.58 0.58 0.57 0.57 0.58 0.58 0.58 0.58 0.58 0.38 0.58 0.58 0.58 0.87 0.88 0.89 0.89 0.89 0.88 0.89 0.88 0.88 0.88 0.88 0.89 0.89 0.89 0.88 0.89 0.89 0.83 0.89 0.89 0.27 0.45 0.28 0.45 0.28 0.46 0.28 0.46 0.28 OA5 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.46 0.28 0.45 0.28 0.45 0.28 0.45 0.28 0.46 0.28 0.45 0.28 0.45 0.28 0.45 0.99 1.00 1.01 1.01 1.01 LO0 1.01 1.01 1.01 1.00 1.00 1.01 1.02 1.01 1.01 1.01 1.01 1.01 1.01 1.01 0.32 0.33 0.33 0.33 033 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.33 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 1.04 0.07 1.05 0.06 1.06 0.07 1.06 0.07 1.06 0.07 1.06 0.07 1.06 0.07 1.06 0.07 1.06 0.07 1.05 0.07 1.05 0.07 1.06 0.07 1.07 0.07 1.06 0.07 1.06 0.08 1.06 0.07 1.06 0.07 1.06 0.07 1.06 0.08 1.06 0.08 Note:
7-d lCa, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean ICs5).
S = Standard deviation of the IC-15 values.
Laboratory Control and Warning Limits Laboratory control and warming limits were established using the standard deviation of the Icjs values corresponding to the 1oth and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
SAm = Standard deviation corresponding to the 10 percentile CV. (S5,o = 0.12)
SAs Standard deviation corresponding to the 25t' percentile CV. (SZ = 0.21)
USEPA Control and Warning Umits
$aýs = Standard deviation.corresponding to the 7 5" percentile CV. (S*.s = 038)
SAjn-Standard deviation corresponding to the 90te percentile CV. (SA = 0.45)
CV = Coefficient of variation of the IC25 values.
USEPA. 2000. Understanding and Accounting for Method Vaiablity In Whole Effluent ToxdtityApplicatlols Under the National Polletant Discharge uEtlnatien Program. EPA-83-R-M-003. US EnvImnsmental Protecton Agency, Cinctinat, OH.
File: ppkortI_091713.xIsx Entered by' J. Sumrwer Reviewed b-5
(
Environmental Testing Solutions, Inc.
Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.
1.25 1.00 0.75 0.50 0.25.
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(
USEPA Acetac riei (> 0.25 mg per surviving larvae)
I I
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1 II I
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'0 wCD 4a 0
0.
30 20 10 0
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Kentucky Acceptance Limit (< 30.0%)
I Upper I
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USEPA Upper PMSD Bound (90t" percentile < 30.0%)
30 0.
C',
20 F 10 I
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I Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD)
I--
Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) age 82 of 105 Graphs generated from associated excel spreadsheet.
Excel spreadshee entered by: J. Sumner Reviewed bvy
Precision of Endpoint Measurements Pimephales promelas EnvlronmentalTestlngSolutlons. nc.
Chronic Reference Toxicant Data Test number Test Control Control Mean CT CV C1 MSD PMSD CT Survival Growth Km/larv' for Control Growth for Control K
for PMSD
(%/)
(mg/larvae)
Growth CV (%)
1 08-07-12 97.5 0.660 6.7 0.06 8.7 2
08-14-12 100 0.533 0.597 5.4 6.0 0.07 13.1 10.9 3
09-11-12 100 0.599 0.598 4.2 5.4 0.06 10.1 10.6 4
10-02-12 100 0.558 0.588 3.9 5.1 0.04 7.0 9.7 5
10-16-12 97.5 0.550 0.580 6.7 5.4 0.09 15.5 10.9 6
11-06-12 100 0.615 0.586 5.9 5.5 0.04 7.1 10.2 7
12-04-12 100 0.601 0.588 3.3 5.2 0.06 10.1 10.2 8
01-08-13 100 0.549 0.583 3.3 4.9 0.06 11.0 10.3 9
01-15-13 100 0.532 0.577 4.2 4.9 0.07 13.0 10.6 10 02-05 100 0.487 0.568 7.4 5.1 0.06 12.8 10.8 11 03-05-13 100 0.504 0.563 8.5 5.4 0.07 14.7 11.2 12 03-05-13 100 0.564 0.563 9.1 5.7 0.10 16.9 11.7 13 04-02-13 100 0.582 0.564 6.5 5.8 0.12 19.9 12.3 14 05-07-13 100 0.651 0.570 7.8 5.9 0.08 11.9 12.3 15 05-14-13 100 0.599 0.572 5.7 5.9 0.08 12.8 12.3 16 06-11-13 100 0.635 0.576 5.5 5.9 0.06 9.2 12.1 17 07-09-13 100 0.615 0.578 4.9 5.8 0.09 14.5 12.3 18 08-06-13 100 0.666 0.583 8.3 6.0 0.08 11.4 12.2 19 09-10-13 100 0.538 0.581 6.1 6.0 0.06 11.8 12.2 20 09-17-13 100 0.594 0.582 4.7 5.9 0.10 16.2 12.4 Note:
CV = Coefficient of variation for control growth.
Lower CV bound determined by USEPA (1 0'h percentile) = 3.5%.
Upper CV bound determined by USEPA (90t percentile) = 20%
MSD = Minimum Significant Difference PMSD.= Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10' percentile) = 12%.
Upper PMSD bound determined by USEPA (90't percentile) = 30%.
Cr = Central Tendancy (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interdaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-O05. US Environmental Protection Agency, Cincinnati, OH.
File: ppkclcr.091713.xlsx Entered by: J. 'Supner age 83 of 105 Reviewed by:
%\\
SET Page 1 of 6 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas PpKCICR Test Number:.2:_5 Dilution preparation information:
Comments:
KCI Stock INSS number:
1 W-6 12 (Ti Stock preparation:
50 g KCI/L:
Dissolve 50 g KCI in I-L Milli-Q water.
Dilution prop (nig/L) 300 450 600 750 900 1050 Stock volume (mL) 6 9
12 15 18 21 Diluent volume (mL) 994 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 1000 Test organism information:
Test information:.
Organism age:
It. is A"
i1VLS Randomizing template:
Date and times organisms were Ock0*
6i" t3 II6bC)
Incubator number and born between:
shelf location:
b Organism source:
ATM MmA 1 16-th3)
Artemia CHM number:
CA Ki-'i.o Drying information for weight determination:
Transfer vessel information:
pH =
-V. 60 S.U.
Date / Time in oven:
6k IL4-3 Its Temperature =
I.A
°C Initial oven temperature:
60 ".
Average transfer volume:
Date / Time out of oven:
1651.t 1bS I * ~I l@S
- Final oven temperature:
6 -L Total d*ying time:
"- ioq t-Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time
.!Analst Time Analyst 0
Ov %Of 1*.I A
- 4.
AL%014c I__
M-a' 1 ciao tmu 1oC
~
~
~
i 4
2 01-6-45 onsf 4
ON-I-t. t IN 5
o4 ti" 0400 Al lots 6
MAIN-I'S ni o*
- it ioCo I
I oq.-Lq-is Control information:
Acceptance criteria-Summary of test endpoints:
% Mortality:
.:r, 20%
7-day LCo 1"
Average weight per initial larvae:
.0..
NOEC Average weight per surviving larvae:
C.
A
> 0.25 m arvae LOEC ISO ChV (010O.R ChY C)
-~
-I-IC74 I
age 84 of 105 SOP AT21 -Exhibit AT21.1, revision 06-01-11
- ETS SE nvlronfliefllTeSUflg Sdohion. t nc, Page 2 of 6 Species: Pimephales promelas PpKC1CR Test Number:
Survival and Growth Data Day Control 300 m KCFlL 450 mn KCFL A
B C
D E
F G
H I
J K
L 0to 1o 10 10 10 10 10 /D 10 10 10 1()
1 (0
/0 (0
10 10 I.0 to 0
to 10 10 10 1
10 to 10 I1 1O 10 10 10 10 10 to 10 4
1 t to 110 to 10 10 to 10 110
/0 5
10 10 10
'o 1C 10
/Q
'0 10 lO 0 1/0 5
1
.0 j
10
- 10) too 10 b to: 10 6
It) 10110
.113 to-10 1-1 o
10 1
D 1.
to to 10 to 010 o t1 1 10 A = Pan weight (mag)
Tray color code::
RON Analyst.
ICM W
15 16.4q 15-11.1' 10[I'0 1 V55 15.5tj 14-51o
[
1"S-*q516.%T 10.CI Date:
pg.l54'*-
B = Pan + Larvae weight (mag)
Analyst:
L%1 21o53 L
- 2. - Zol, Z2.lIo zCC1Z.
2.1.1' 22.1.31 23..
Date:
O Q -XI?
- V374 7
"3" C = Larvae weight (mg) = B - A Hand calculated.
s.m koL%
Ial~.
.E 1-uA
-6x-I~AM U-4 Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
nt 'I is N
a 0
N I,
V N
0
%0 0%
'0 Average weight per initial Percent number of larvae (mg) reduction 0
S, "4
from control M)
I SoT 0.1, k7.
Comment codes: c = clear, d = dead, fg = fingus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
(
age 85 of 105 SOP AT21 -Exhibit AT21. 1, revision 06-01-11
3 nrnvtmnmntlTesting Solutions, Imu Page 3 of6.
Species: Pimephales promelas PpKCICR Test Number.
A Survival and Growth Data Day 600 mg KCI/L 750 mg KCI/L 9090D m KCV/L M
N 0
P Q
R S
T U
V W
X 0
10' to IU t~Q o 10 1
- 0) 1Q 1 0t 1
,0 t(C)
(
(0 l0 tO tO I
61A I 0 I
2 10 Ck AL V
~ 10 51c)
I~
101 C)
(C V
6 it)
,o 10 to 0"..
- 7.
7!
A= Pan weight (mg)
Tray color code::
O Analyst:
0 IM*15. 33.5 jl.Z 15.q3 164.. 153o nl*.tb z L iq.% 4.'lt 9 I oAS 1561 Date: _
IT.!*"
'l*
B = Pan + Larvae weight (mg)
Analyst:
1*1Z 22 z59 zz*1 2O.5 7.oR 1j.*Z ZZi is 5.
tb.3b Ih.Z 1'._l Io3 Date:
oq--7.1%
C = Larvae weight (mg) so B - A Hand calculated.
-. 4 1.U
't S
.h O'S $.&Q 5.S l
-1.S10 1.40 L.S4 o.&?r
- .01-Analyst:
Weight per Initial number of larvae (mg)
= C/Initial number of larvaef,
,V Hand calculated.
P'e 1
9E Analyst:
o, o
O" Average weight per initial Percent number of larvae (mg) reduction 0 610
- 5,67.
o,~qI-
-ze,07.
0..O 7.
from control Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT21 - Exhibit AT2 1.1, revision 06-01-11 age 86 of 105
I :ETS 0
nio nna etig,* to r
Pae sf64
'P 4-*
Species: Pimephales promelas PpKCICR Test Number:
V71-5 Survival and Growth Data Day 1050 m, KCi/L 77Y Z AA I BB 0
blob1 10 3
'4K 4
5
.6
-Al 7
A = Pan weight (mg)
Tray color code::
VV%-%
14 Analyst:,.
14-%16 -.
4 If~u3 i153D
ý 1
Date:
Oq' B = Pan + Larvae weight (mag)
Analyst:
141-w>.5 I 15,0
- Date:
0 "LI"Zn
- 06 C = Larvae weight (mrg) = B - A Hand calculated.
0.'
0I Onl1 0.*&1 Analyst:
Weight per initial number oflarvae (mrg)
= C /Initial number of larvaex Hand calculated.
Analyst:
Average weight per initial Percent number.of larvae (mg) reduction 5ol 7q from control
(%)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments"
- page 87-of 105 SOP AT21 - Exhibit AT21.1, revision 06-01-11
(Q O
-Pimephales promelas Chronic Reference Toxicant Test o
W EPA7821-R-02-013, Method 1000.0 (Ai Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.
Test number 273 Test dates:
September 17-24, 2013 C-(Io
.I le(-
KI)
R.POlM i hftd.i-b-hf flnlnm6e5f A.P
,lW bW 6= P Ln4+
L-
%M a(mg)
WnIeflsunt*vln M-uM wtda/Su*nn CMwntderh Wn'k0t/lnnwgrn.nbnf Mann nunla(%] MAnn h.taW G, ~mtaf-nhdl1o P-,tmductfonftem Ial.
h-
-Igt(e A-B w mbennfhnn.l-a)
.. eA.,flwwVr nm) 0-lana W-1-
nn
- berofl~na l't onenl (ul A
10 10 15.72 21.53 5.81 0.581 0.581 contro B
10 10 15.49 21.70 6.21 0.621 0.594 4.7 0.621 100.0 0.594 4.7 Not applicable C
10 10 15.18 21.32 6.14 0.614 0.614 D
10 10 15.13 20.74 5.61 0.561 0.561 E
10 10 16.40 22.16 5.76 0.576 0.576 300 F
10 10 16.35 22.13 5.78
- 0.
639 11.7 0.578 100.0 0.639 11.7
-7.5 0
10 10 15.54 22.27 6.73 0.673 0.673 H
10 10 15.03 22.31 7.28 0.728 0.728 1
10 10 14.56 21.79 7.23 0.723 0.723 450 J
10 10 15.45 22.31 6.96 0.686 0.711 8.1 0.686 100.0 0.711 8.1
-19.6 K
10 10 16.68 24.72 7.84 0.784 0.784 L
10 10 16.90 23.39 6.49 0.649 0.649 M
10 10 15.84 22.90 7.06 0.706 0.705 600 N
10 9
16.33 22.59 6.26 0.696 0.696 1.9 0.626 90.0 0.627 10.7
-5.6 0
10 9
16.52 22.67 6.35 0.706 0.635 P
10 8
15.43 20.65 5.42 0.678 0.542 Q
10 6
16.24 20.97 4.73 0.788 0A73 750 R
10 6
15.30 18.82 3-52 0.587 0.713 12.3 0.352 60.0 0.428 18.6 28.0 S
10 7
17.20 22.37 5.17 0.739 0.517 T
10 5
14.80 1W.50 3.70 0.740 0.370 U
10 2
14.96 16.36 1.40 0.700 0.140 900 V
10 2
14.97 16.52 1.55 0.775 0.155 17.5 0.120 28.1 79.8 W
10 1
16.45 17.27 0.82 0.820 0.082 X
10 2
15.71 16.73 1.02 0.510 0.102 S
10 1
14.56 15.05 0.49 0.490 0.049 1050 Z
10 1
16.43 17.14 0.71 0.710 0.487
- 4.
0.071 7.5 0.037 83.5 93.9 AA 10 1
15.30
.15.56 0.26 0.260 0.026 as 6 10 0
0.00 0.00 0.00 0.000 0.000 Dunnett'$ MSD value:
PMSD:
0.0965 16.2 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD Is the minimum percent dffermnce between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSO data from EPAs WET Interlaboratory Variability Study (USEPA, 2002a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: Interlaborabory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821.9'01-004 and EPA-821-B-01-005. US Envimnmental Protection Agency, Cincinnati, OH.
File: ppkclu 0o917ll3.xlsx EMtered by-. S.nse Reviewedb- -T
S-ETS Eni0 mna etn ouln r Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:.
9/17/2013 Test ID:
PpKCICR Sample ID:
REF-Ref Toxicant End Date:
9/24/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potasslum chloride Sample Date:
Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-mg/L 1
2 3
4 D-Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 500 1.0000 0.9000 0.9000 0.8000 750 0.6000 0.6000 0.7000 0.5000 900 0.2000 0.2000 0.1000 0.2000 1050 0.1000 0.1000 0.1000 0.0000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV%
N Sum Critical Reap Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 0
40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 0
40 600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4
12.00 10.00 4
40 "750 0.6000 0.6000 0.8872 0.7854 0.9912 9.469 4
10.00 10.00 16 40
- 900 0.1750 0.1750 0.4282 0.3218 0.4636 16.570 4
10.00 10.00 33 40
- 1050. 0.0750 0.0750 0.2810 0.1588 0.3218 28.997 4
10.00 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shaplro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.82709 0.896
-0.321 1.98788 Equality of variance cannot be confirmed Hypothesis Test (1-tall. 0.05)
NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Maximum Ilkelihood-Probit Parameter Value SE 95% Fiduclal Limits Control Chl-Sq Critical P-value Mu Sigma Iter Slope 12.0198 1.46854 9.14144 14.8981 0
0.98032 9.48773 0.91277 2.8896 0.0832 3
Intercept
-29.732 4.25763
-38.077 -21.387 TSCR 1.0 Point Probita mg/L 95% Fiduclal Limits 0.9 ECO1 2.674 498.661 425.225 548.18 ECOS 3.355 565.923 603.309 610.943 0.8.
EC10 3.718 606.712 550.101 647.921 0.7:
EC15 3.964 636.881 583.712 674.578 EC20 4.158 660.06 611.535 698.9430.6 EC25 4.328 681.535 636.108 717.115 a0.5, EC40 4.747 738.798 700.119 773.188 EC50 5.000 775.538 739.206 811.728 0.4 EC60 5.253 814.105 778.013 854.887 0.3:
EC75 5.674 882.507 841.512 937.937 0.2, EC8O 5.842 911-219 866.593 974.809 0.2 EC85 6.036 945.868 895.985 1020.52 0.1 EC90 6.282 991.342 933.466 1082.16 0.0 EC85 6.645 1062.79 990.585 1182.02 1
10 100 1000 10000 EC99 7.326 1211.01 1104.66 1398.24 Dose mg/L Dose-Response Plot I
age 89 of 105 File: ppkclcr 091713.xisx Entered by: J. Sum Reviewed by:
- ET Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
911712013 Test ID:
PpKCICR Sample ID:
REF-RefToxicant End Date:
9124/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potasslum chloride Sample Date:
Protocol: FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-mglL 1
2 3
4 D-Control 0.5810 0.6210 0.6140 0.5610 300 0.5760 0.5780 0.6730 0.7280 450 0.7230 0.6860 0.7840 0.6490 600 0.7060 0.6260 0.6350 0.5420 750 0.4730 0.3520 0.5170 0.3700.
900 0.1400 0.1550 0.0820 0.1020 1050 0.0490 0.0710 0.0260 0.0000 Transform: Untransformed 1-Tailed Isotonic ConcmglL Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.5943 1.0000 0.5943 0.5610 0.6210 4.747 4
0.6478 1.0000 300 0.6388 1.0749 0.6388 0.5760 0.7280 11.704 4
-1.056 2.290 0.0965 0.6478 1.0000 450 0.7105 1.1955 0.7105 0.6490 0.7840 8.102 4
-2.758 2.290 0.0965 0.6478 1.0000 600 0.6273 1.0555 0.6273 0.5420 0.7060 10.707 4
-0.783 2.290 0.0965 0.6273 0.9682 750 0.4280 0.7202 0.4280 0.3620 0.5170 18.636 4
0.4280 0.6607 900 0.1198 0.2015 0.1198 0.0820 0.1550 28.083 4
0.1198 0.1846 1050 0.0365 0.0614 0.0365 0.0000 0.0710 83.535 4
0.0365 0.0563 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test Indicates normal distribution (p > 0.01) 0.95791 0,844 0.14514 -0.8584 Bartlett's Test indicates equal variances (p = 0.51) 2.30131 11.3449 Hypothesis Test (I -tall, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600
>600 0.09651 0.1624 0.0096 0.00355 0.09239
- 3. 12 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL(Exp )
Skew IC=5 608.89 34.26 456.48 641.99
-1.0904 1C10 633.27 19.82 543.62 677.90
-1.2476 IC15 657.66 18.99 597.12 724.18 0.0394 1.0 IC20 682.05 21.25 633.33 772.39 0.8531 0.9 IC25 706.43 22.45 654.16 789.15 0.5884 0.8 IC40 769.12 15.78 714.51 811.15
-0.2718
- 0.7 IC50 800.65 12.40 757.36 833.52
-0.3125 0.6 3 0.5 o 0.4 9 0.3 0.2 0.1
- 0.0
-0.1
-0.2
-0.3 0
500 1000 1500 Dose mgIL Dose-Response Plot I-1-tall, 0.05 level of significance
- Page 90 of 105 File: ppkclcr 091713.xlsx Entered by: J. Surner Reviewed by: __t
0:T 4)Env~ramntnIT.,tngSo0ot~an%,,C Page 5 of 6 Species: Pimephales promelas PpKC1CR Test Number:
Daily Chemistry:
I Day
- (Analyst identified for each day, performed pHi. D.O. and cofiductivitv mcai m
Analyst I
.Concentration Parameter pH (S.U.)
~--
DO (mg/L)
Conductivity (n1hos/cm)
CONTROL
- Alkaiinity (me CaCO.fU
- Hardness
- (ME CaCOI/L)
- Temperature (M) m pH (S.u.)
- 4. -~-~--
41 4
DO (rng/L) 300 mg KCI/L iiinv Conductivity (umhos/cm*
01 4Im)
- Temperature (0C) pH (S.U.)
T ~
DO (mg/L) 450 mg KCI/L Conductivitymhos/cm
- Temperature (CC)
L,. A,_.
pH (s.u.)
4--'~-=---4~
DO (mg/L)
~4 600 mg KCI/L Conductivity (umhoslcm)
=
- Temperature (0C) 9 U
Ip -
pH (S.U.)
DO (mgtL) 750 mg KCl/L Conductivity (umhos/cm)
- Temperature (0Q) a a
pH (S.U.)
- -4~'---.4I DO (mg/L) 900 mg KCl/L K
1 Conductivity (umhos/cm) 4
- Temperature (0C)
U U
pH (S.U.)
1050 mg KCI/L DO (mg/L)
-~
-~---r Conductivity (iimhos/cm)
L
~J
- Temperature (00)
STOCK Conductivity STOCK____
- n.
Initial Final..
t II 'Finali ii tia.F l
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page "1.
Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by':.
SOP AT21 - Exhibit AT21., revision 0601* -
- Page 91 of 105 1
ET (5I~ Er1VlronmefltaT.Itdng Sodt1orn,1m Species: Pimephales promelas 3
- 1. Concentration Paramer DO (mg/L)
Conductivity CONTROL
- Alkalinity (Mg CaC0 3/L)
- Hardness
- Temperature (Dc)
V41.*
Page 6 of 6 PpKCICR Test Number 2.1-S Day 4
5 6
Liko
.At.
.II 64 Icewj 1kL ivo VA.1 VA -4 6
Vk. 1:
1~1
)
2 pH(S.u.)
A%_7,.0 DO (mg/L) 450 mg KCI/L Conductivity (mhos/c)
%1RH
- Temperature V"
I.,".,
"~~
pH (S.U.)
q.5;!
(Sap T
.2 DO (mg/L)
.4.b9V 600 mig KCI/L (C o s)Cnductvy 1I3)1 o"o 0"
750 mg KCI/L Conductivity (col D_
C_
- Temperature "iA"
-" "S
)A-b v" 's L-.
L'I.-
(Oc) pH (S.U.)
St -3 01Z7.E DO (mg/L)
J~t'.~
19 1
900 mg KCI/L Conductivity
- 19z,
- Temperature UZ4
. ".1 l
Z.
t-: a, (OC) 1050 mg KCI/L Conductivity
- Temperature
-AZ.
"LO zq]
_.(c1, Initial 11 Final I Initial 11 Final I Initial I Final I Initial 11 Final peratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
AJJ~a-itty and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
i.
K*kyE I~
.SOP AT21 - Exhibit AT21.1, revision 06-01-11 (11-_"Page 92 of 105
61 Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.
1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 I
I I
I II I
I I
I I
I I
I I
I I
I I
I USEPA Control Limits (+/-2 Standard Deviations)
I I
I I
f I ~
I I
I I
I I
I I
I I
I __
%n 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 AQ I
II I
I I
I I
I I
I I
I I
I I
I I
USEPA Warning and Control Limits (75th and 90 Percentile CVs)
S S
0
- -e--
~-- -----------
~S S
p o.o......
o.o o
o I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
i I
I I
I I
I i
I I
I l
I I
I I
I Laboratory Warning and Control Limits (10th and 2 5 th Percentile CVs)
...................... o..............
o...................*.......*.
I I
I I
I III I
I I
I*
I I
I_
f I
I I
I V,0 N" '~%I, WN
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aq,^,"
SA VN
-1A tl~
11,85 ON 6A 60 Test date 7-day IC.5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC 2 5)
Warning Limits (mean IC2 5 + SA. 0 or S k7)
......... Control Limits (mean IC25 :+/-: SA2s, SA90, or 2 Standard Deviations)
R ag by i/'age 93 of 105 Graphs generated from associated excel spreadsheet Excel spreadsheet entered by: J. Sumner Reviewed by:
40k OE.TtS Environmental Testing Solutions, Inc.
Ceriodaphnia dubla Chronic Reference Toxicant Control Chart Test number Test date 7-day IC.s CT (g/L NaCi)
(g/L NaCI) 1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 05-08-12 05-08-12 06-05-12 07-10-12 08-07-12 09-11-12 10-02-12 11-06-12 12-04-12 01-08-13 02-05-13 03-05-13 04-02-13 05-07-13 05-07-13 06-04-13 07-09-13 08-06-13 08-06-13 09-10-13 1.06 1.05 1.05 1.07' 1.08 1.06 1.04 1.05 1.06 1.07 1.05 1.05 1.07 1.07 1.06 1.09 1.06 1.07 1.05 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 1.06 State and USEPA S
Control Limits Sý CT-2s CT+zS 0.01 1.05 1.08 0.09 0.01 1.05 1.08 0.09 0.01 1.05 1.08 0.09 0.01 1.05 1.08 0.09 0.01 1.05 1.06 0.09 0.01 1.05 1.08 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.09
- 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.08 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.09 0.09 0.01 1.04 1.08 0.09 0.01 1.04 1.08 0.08 0.01 1.04 1.08 0.08 0.01 1.04 1.08 0.06 Laboratory Warning Limits CT-S S.
CT+So Laboratory SAuM Control Limits CT-Sý2 s CT ÷55s USEPA SAJSs Warning Limits CT - S,*
CT + SA, USEPA SAo Control Limits CT - SAO CT÷S.o CV 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 3-15 1.15 1.15 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 1.24 1.24 1.24 1.24 1.25 1.25 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.59 0.58 0.58 0.59 0.59 0.59 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.59 0.58 0.58 0.58 0.58 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 Note:
7-d lCz = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodophnio reproduction for the test population.
CT = Central tendency (mean IC2s).
S = Standard deviation of the IC. values.
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the ICs values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
SA.s = Standard deviation corresponding to the 1 0e percentile CV. (S&IO = 0.08) s = Standard deviation corresponding to the 2 5& percentile CV. (Sý = 0.17)
USEPA Control and Warning Limits Sx75 = Standard deviation corresponding to the 7P percentile CV. (S,
= 0.45)
= Standard deviation corresponding to the 9 0t percentile CV. (So
= 0.62)
CV= Coefficient of variation of the ICý values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICR_091013.slsx Entered by: J. SjJmner Reviewed by:
A
- Envlronmtental Testing Solution.. Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 0
U 0
0 0Q C4 I
40 35 30 25
.I I
.I.I.I......................
Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
I I
i I
I I
I i
I I
I I
I I
I i
I i
I 20 15 40
"&! 30 20 10 50 40 S 30 20 10 0
I I
I I
l I
I I
.I I
I I.
I I
I I
I I
I North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
I...
T...
T
.i..
... T....
...i....1..
1 T
I I_
I I
I I
I I I
I I
I I
I I
I I
I USEPA Upper PMSD Bound (9 0t1 percentile < 47.0%)
A.o
- O I
JAO
- e oeee e
l o
e eee eo e
e Test date
-e----- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD)
......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
ý.b,.age 95 of 105 Graphs generated from associated excel spreadsheet.
Excel spreadsheet entered by. J. Sumner Reviewed by:
0
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Test uest Test date number Control Control Mean Survival Reproduction
(%)
(offspring/female) 1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 05-08-12 05-08-12 06-05-12 07-10-12 08-07-12 09-11-12 10-02-12 11-06-12 12-04-12 01-08-13 02-05-13 03-05-13 04-02-13 05-07-13 05-07-13 06-04-13 07-09-13 08-06-13 08-06-13 09-10-13 100 100 100 100 100 100
- 100, 100 100 100 100 100 100 100 100 100 100 100 100 100 31.7 32.4 32.1 31.2 29.2 30.7 32.5 32.5 31.9 30.8 32.1 31.4 28.7 30.4 32.2 29.7 30.7 31.0 33.9 29.2 CT for Control Mean Reproduction (offspring/female) 32.1 32.1 31.9 31.3 31.2 31.4 31.5 31.6 31.5 31.6 31.5 31.3 31.3 31.3 31.2 31.2 31.2 31.3 31.2 CV CT for Control
(%)
Reproduction CV (%)
MSD PMSD
(%)
for PMSD (%)
CT 6.1 5.1 4.0 4.7 3.5 6.2 3.0 3.6 5.2 5.5 6.3 4.8 5.9 8.4 6.2 5.0 6.9 4.6 5.5 5.5 5.6 5.1 5.0 4.7 4.9 4.7 4.5 4.6 4.7 4.8 4.8 4.9 5.2 5.2 5.2 5.3 5.3 5.3 5.3 1.9 2.0 2.3 2.0 1.9 2.3 1.5 1.8 1.9 2.0 2.0 1.7 1.9 2.2 2.4 1.7 1.6 1.7 2.1 1.6 6.1 6.1 7.1 6.3 6.6 7.4 4.6 5.4 6.0 6.5 6.1 5.6 6.8 7.3 7.3 5.7 5.3 5.3 6.1 5.4 6.1 6.4 6.4 6.4 6.6 6.3 6.2 6.2 6.2 6.2 6.2 6.2 6.3 6.3 6.3 6.2 6.2 6.2 6.1 Note:
CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (10th percentile) = 8.9%.
Upper CV bound determined by USEPA (90 th percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically siRnificant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10 th percentile) = 13%.
Upper PMSD bound determined by USEPA (90'h percentile) = 47%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
age 96 of 105 File: CdNaCICR_091013.xlsx Entered by: J. Sutpner Reviewed by:
U
'4 I&T li Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCICR #: jj*
Dilution preparation information:
Comments:
NaCI Stock INSS number.
16%
a'o*_
Stock preparation: '
100 g NaCI/L:
Dissolve 50 g NaCI in 500 mL Milli-Q water.
Dilution prep (mg/L)
.600 800 1000 1200 1400 Stock volume (mL) 9 12 1
15.
18 21.
Diluent volume (mL) 1491 1488 1485 1482 1479*
Total volume nmL) 1500 1500 1500 1500 1500 Test organism source information:
Test information:
Organism age:
< 24-hours old Randomizing template color:
&4.,
Date and times organisms were born 01 O00 'M O'c U Incubator number and shelf between:
location:
Culture board:
%,I A Replicate number:
1 2
3 [ 4 1 5 6 17 8
9 10 YWTbatch:
Culture board cup number:
tO I I [
] I "a
1
" I.
Transfer vessel information:
pH -'i.a& S.U.
Temperature
-1
°C Selenastrum batch:
Average transfer volume (mL):
-L.
Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0
6 + 10 A__.
A-A__
ot o%-.L-
%'so 6
%L. I ac t-.. IN Control information:
Acceptance criteria Swn~mary of test endpoints:
% of Male Adults:
j*7,
- 20%
7-day LC50
- Ittt
% Adultshaving 3rd Broods:.
1 081,
>80%
NOEC 3:
% Mortality:
- 01.
- 20%
LOEC "Z 0 Mean Offspring/!Female:
"ZI "*
.!5.0 offpring/female ChV IO*
%CV:
S.7-
<v40.0-%
ICs tage 97 of 105 SOP AT14 - Exhibit ATI 4.1, revision 06-01-.11
i[
S a
0
~r.L f-I,--,-
I.
f I)
TS Specie*
CONT Page 2 of 6 s: Ceriodaphnia dubia
'ROL CdNaCICR #:
15___
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 Young produced C
C) 00 0*
Adult mortality '*_
1 Young.
K prdue n
p~
3 Adult mortality F F. FE..
2 Young. produced I
0.~ 0
~
.. oT___
Adult mortality t
_II_
4 Young produced
%A Adult mortalityI 1
5 Young produced___
x7Z
~
~
_x_
i __
Adult mortality 6
Young produced r)
~
-I Total young produced L, n b
N10
" L*1
-Lot m"L.
-LI Final Adult Mortality C.
- .. C I
X for 3rd Broods Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:j
% Mortality:
70 Mean Offspring/Female:
"7..-L'I 600 mg NaCIIL Survival and Reproduction Data S"__
_Replicate number Day 1
1 2
3 4
5 6
7 8
9 10
.1 "
- Young produceld i
0 o
0
_ Adult mortality
,.T..
I %.-
i 7
2 Young produced C) 0 lr 4 "
Adult mortality L
3 1 Young produced 0
0 1 01 0 Adult mortality 51 Young produced Adult mortality 1
o 6 Young produced 0~~.I 1 0 01 101 LX..10.L.%
Adult mortality I_
%- IC L 7 ?
7 t Young produced 1-1 ts
%-A it.
iS is
%" i
- b i$I Total young produced.
_57-
-b
-b 7
b i
3U Final Adult Mortality C
C7 I. \\-
I J
C I
Note: Adplt mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
Mean Offspring/Female:
- age 98 of 105
- % Reduction from Control:
I o SOP AT14 - Exhibit AT14.1, revision 06-01-11
E TS Specie 800 m Page 3 of 6 s: Ceriodaphnia dubia g NaCI/L CdNaCICR #:
- 5 Survival and Reproduction Data Replicate number Day 1_1 2
3 4
5 6
7 8
9 10 I
Young produced 0
0(
0 i 0
)
Adunit mortality
- j_
u..
\\.
I Adult mortality 2
Young produced ty I6 G
S Adult mortality
'2'
_t.Yo d
I 3
Young produced m
- y.
0 1
YnreAdult mortality
'.J
[ \\1..
I
.-] "...
I Young produced
%A
_j Adult mortalityj J
6 Young produced 101 n) 0 I 01... 10101) 0 Lrc..
___fAdult mortality
$,-\\_~~J~~_______
7 Young produced k
L.
Total young produced
-A
-I 21 2 ",
Final Adult Mortality
, I Note: Adult mortality (L =live, D = dead), SB = split brood(single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
" Concentration:
% Mortality:
1 Mean Offspring/Female:
-L4. 6
% Reduction from Control:
Z 7. 1.
1000 mg NaCIIL Survival and Reproduction Data Replicate number Day 1 1 2
3 4
5 6
7 8
9 10 1*
Young produced 5
"1-t 0
0-Adult* mortality 2
Young produced.Q Adult mortality 3
Young produced
- 1. 0C 0*,'
C)
Ii*I Y
mQ
[
0-_. 0 Adult modality
__I
\\-
-I
'-A-I\\--
I
\\- I%--
4
[Youngproduced II 1 1b.
%A 1 %
Adult mortality k-_
\\]
\\
li *
[ t J..
[ Young produced i, I rL.
Tl \\
I
]- D V*
Adult mortality k-TC 1,..
\\ý:L.
Adult mortality T
OL.
I L_
I_%--_
L__
- 6.
Young produced\\:S I
Total young produced j
( is si I
i 21
-X%
2(
- 2.
vj Final Adult Mortality K.
t I. -
\\
, I Note: AdUlt mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
Mean Offspring/Female:
- Page 99 of 105 I % Reduction from Control: 1 3,
Tb SOP ATI4 - Exhibit ATI4.1, revision 06-01-11
Specie 1200 n Page 4 of 6 s: Ceriodaphnia dubia g NaCJ/L CdNaCICR#: M165 Survival and Reproduction Data I
I Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
Young produced C)
C) 0__
)
0 0
C)
Adultmortality
\\.-
l_.
2 Young produced I Jl f0 TZ I LIJ O I n
__IAdult mortalityJ'I'
'1 _-
4 Young produced 1o JAdult mor~ity L
-1 14*
'I'-I'.I-
-I 5
Young produced S
~
Adult mortality I -I
[
2 -
'k-I 1" I
5 produced I *.
I:
1 j
l *,_ IA '
6 Young produced
. ]*0o 1
_1___
0
° T Adult mortality I
'l'-I
'1 7
Young produced 1
I.
O I '
Total young produced t "
oT L I
j O
iD i
8
.2. I Final Adult Mortality..
j
\\
t
\\-
r-I Note: Adult mortality (L live, D = dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration: "
% Mortality:
Mean Offspring/Female:
I
% Reduction from Control:
61,S7.
1400 mg NaCI/L Survival and Reproduction Data.
Replicate number Day 1
2 3
4 5
1.6 7
- 8.
9 10 I
Young produced 101_O j
C 5
0L
- 0) 0o
.1 Adult mortality L_..
J 2
j Young produced 0I
_..0 Adult-mortality i
Young produced Adult mortality 2
-l 4
Young produced 7
.'"""-i..
Adult mortality EEI I
V 4
YoungPproduce d 0 C)
C o10 Adult mortality I 7-I jI
_cI J
.I."ung"r.d.ced tI*
I 7
JYoung produced 1} C)"-
Adult lit
'.[. IIj*j..
H 1
Total young produced lo" p
[ o T
.*i c*
t "
c Final Adult Mortality
\\4; 1"
- -c-. -
Note: Adult mortality carried over with adult
\\*__age 100 of 105 (L = live, D = dead), SB- = spilt brood (single brood split between two days), CO = cany over (olfspnng during transfer).
Concentration:
% Mortality:
7.
Mean Offspring/Female:
2.0
% Reduction from Control:
5.2 7.
SOP AT14 - Exhibit AT14.1, revision 06-01-11
0 Environmental Testing Solutions, Inc.
Control Verification of Ceriodaphnia Reproduction Totals Day Re licate number Total y 1 2
"3 4
5 6
7 8
9 10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0
- 0.
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
3 4
4 3
3 3
4 4
3 3
34 5
10 10 11 13 10 10 11 12 10 10 107 6
0 0
0 0
0 0
0 0
0 0
0 7
14 16 14 15 17 14 14 16 15 16 151 Total 27 30 29 31 30 27 29 32 28 29 292 600 mg NaCI/L Day Replicate number Total Dy 1 2
3 4
5 6
7 8
9 10 Toa 1
0 r
0 0
0
- 0 0'
0 0 1 0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 2 0
0 0
0 0
0 0
0 0
0 4
4 3
4 4
3 4
3 4
3 3
35 5
11 12 10 10 10 11 10 12 12 12 110 6
0 0
0 0
.0 0
0 0
0 0
0 7
17 15 17 16 15 15 17 13 15 16 156 Total 32 30 31 30 28 30 30 29 30 31 301 800 mg NaCIIL Replicate number 9ota 1 T-2 3
4 5
6 7 1 8
.9 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
3 3
4 3
3 4
4 4
3 3
34 5
12 10 10 11 11 12 11 11 11 10 109 6
0 0
0 0
0 0
0 0
0 0
0 7
14 15 15 15 13 14 13 16 13 15 143 Total 29 28 29 29 27 30 28 31 27 28 286 1000 mg NaCI/L DayReicate number Total 1 1 2
3 4
5 6
7 8
9 10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
- 0.
0 0
4 4
3 4
4 3
3 3
3 3
3 33 5
12 10 12 12 11 10 13 10 10 10 110 6
0 0
0 0
0 0
0 000 0
7 14 12 15 13 14 14 13 15 13 16 139 Total 30 25 31 29 28 27 29 28 26 29 282 1200 mg NaCIIL Day
-~-
Re plicate number Total 1
21 3 14 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
.0 4
2 1
3 2
2 3
3 1
1 1
19 5
5 3
7 4
8 7
4 2
6 3
49 6
0 0
.0 0
0 0
0 0
0 0
0 7
5 2
0 4
0 1
3 5
5 2
27 Total 12 6
10 10 10 11 10 8
12 6
95 1400 mg NaCl/L Day Re licate number Total 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0
- 0.
0 0
0 0
0 4
0 0
2 1
0 1
1 0
2 0
7 5
2 1
0 0
3 0
0 1
0 1
0 8
6 0
0 0
0 0
0 0
0 0
0 0
7 0
0 2
1 0
.1 1
0 0
0 5
Total 2
1 4
2 3
2 2
1 2
1 20 File: CdNaClCR_091013.xlsx Entered by: J. Sumner Reviewed by:
tii (D
0.
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
Test dates:
CdNaCICR #153 September 10-17, 2013 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variation (%)
control (%)
Control 27 30 29 31 30 27 29 32 28 29 100 29.2 5.5 Not applicable 600 32 30 31 30 28 30 30 29 30 31 100 30.1 3.7.
-3.1 800 29 28 29 29 27 30 28 31 27 28 100 28.6 4.4 2.1 1000 30 25 31 29 28 27 29 28 26 29 100 28.2 6.4 3.4 1200 12 6
10 10 10 11 10 8
12 6
100 9.5 22.9 67.5 1400 2
1 4
2 3
2 2
1 2
1 100 2.0 47.1 93.2 Dunnett's MSD value:
PMSD:
1-581 5.4 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (1 0e percentile) = 13%.
Upper PMSD bound determined by USEPA (90'h percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
File: CdNaC[CR_091013.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Sumner Reviewed by:
GET Statistical Analyses Cerlodaphnia Survival and Reproduction Test-Reproduction Start Date:
9/10/2013 Test ID: CdNaCICR Sample ID:
REF-Ref Toxicant End Date:
9/17/2013 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
NACL-Sodium chloride Protocol: FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10
- D-Control 27.000 30.000 29.000 31.000 30.000 27.000 29.000 32.000 28.000 29.000 600 32.000 30.000 31!000 30.000 28.000 30.000 30.000 29.000 30.000 31.000 800 29.000 28.000 29.000 29.000 27.000 30.000 28.000 31.000 27.000 28.000 1000 30.000 25.000 31.000 29.000 28.000 27.000 29.000 28.000 26.000 29.000 1200 12.000 6.000 10.000 10.000 10.000 11.000 10.000 8.000 12.000 6.000 1400 2.000 1.000 4.000 2.000 3.000 2.000 2.000 1.000 2.000 1.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 29.200 1.0000 29.200 27.000 32.000 5.546 10 29.650 1.0000 600 30.100 1.0308 30.100 28.000 32.000 3.656 10
-1.302 2.287 1.581 29.650 1.0000 800 28.600 0.9795 28.800 27.000 31.000 4.423 10 0.868 2.287 1.581 28.600 0.9646 1000 28.200 0.9658 28.200 25.000 31.000 6.431 10 1.447 2.287 1.581 28.200 0.9511
- 1200 9.500 0.3253 9.500 6.000 12.000 22.874 10 28.501 2.287 1.581 9.500 0.3204
- 1400 2.000 0.0685 2.000 1.000 4.000 47.140 10 39.351 2.287 1.581 2.000 0.0675 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 1.01631 1.035
-0.2844 0.05875 Bartlett's Test indicates equal variances (p = 0.14) 8.40602 15.0863 Hypothesis Test (1-tall, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 1000 1200 1095.45 1.58058 0.05413 1504.95 2.38889 0.OE+00 5, 54 Treatments vs D-Control Point IC05 IC10 ICI15 IC20 IC25 IC40 IC50 Linear Interpolation (200 Resamples) mg/L SD 95% CL Skew 1000.35 83.0563 757.247 1010.55
-1.1527 1016.2 5.20163 1004.4 1025.74 -0.1970 1032.06 4.92139 1021.36 1040.67 -0.1160 1.0 1047.91 4.71509 1037.47 1056.54 -0.0148 0.91 1063.77 4.5927 1054.8 1072.49 0.0970 1111.34 4.77162 1101.67 1120.68 0.3403 0.8 1143.05 5.30373 1132.63 1153.76 0.3600 0.70F I086 08 0.2 0.1 0.0
-0.1 0
800 1000 Dose mg/L 1500 Dose-Response Plot 35 1-tail, 0.05 level of significance 25 20
- 0. 16 10 0_
R a
1 f
age 103 of 105
.File: CdNaCICR 091013.xlsx Entered by: J. Sumner Reviewed by V
OET0 W.
Page 5 of 6 Species: Ceriodaphnia dubia CdNaCICR M:
l_
Daily Chemistry:
I (e
or.
r
- p.
Day n
(Analyst identified for-each day. pefformed pHl D.O. and conductivity measurements only.) I I
o0 6
Analyst I Concentration Parameter pH (S.U.)
DO (mg/L) a Conductivity (umhos/cm)
CONTROL
- Alkalinity (rmp CaCOV/U
- Hardness (ma CaCOA/L)
- Temperature (00 SpH (S.U.)
DO (mg/L) 600 mg NaCI/L ConducIvity "mhos/c')
i Temperature (00C) pH (S.U.)
I DO (mg/b) 800 mg NaCIIL I[Conductivity 800 mg NaCI/"
[.(jmhos/cm)
- Temperature (00) pH (S.U.)
1000 mg NaCI/L DO (mg9/b)
Conductivity I
(_.mhos/
c)
O Z
216o "Temperature (00)
IL%-0 I "1&4.R
~l).I.¶ 4
I 0
4
~'.-
lh I
pH (S.U.)
I ".1'4, 1-1.1"4 L..4I DO (mg/L) a o*
.,*Q I
1200 mg NaCI/L Conductivity J'~
V. I
-a I 2,qD:
TLqi er
-I0'
- Temperature (0C) i.,
U i ~
pH (S.U.)
",'I-f 1400 mg NaCI/L Conductivity
-o
,I
("mhos/ c)
- Temperature (0C)
STOCK Conductivity I (,coo Initial I
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and h~jlness performed by the analyst identified on the bench sheet specific fbr each analysis and transcribed to this bench sheet by:
'tage 104 of 105 SOP ATI4 -Exhibit ATI4. I, revision 06 -11
'GT S
Page 6 of 6 Species: Ceriodaphnia dubia CdNaCICR #:
M 5
I Day (Analyst identified for each day, performed PH, D.O. and conductivity measurements only.)
3 4
5 6
I U.-
I Analyst tv-n I
I IA~t II IAJ Concentration j Parameter pH (S.U.)
"n,39J.
DO (mg/L)
I2t.
I-
-F 1'-'
- A I'
1.l CONTROL Conductivity (pimhos/cm)
- Alkalinity (mg CaCr3ne
- Hardness f_____
(-9 CaCO31L)
- 1
- Temperature 1.4 ILA-t a
a 1k
- ~
i II U
d1 pH (S.U.)
,qz_ IF r, 600 mg NaCIJL DO.(mg.L)
O.q
.9 q.
Conductivity o
I___ j
- Temperature (00C
-'Ar' v.41' IpH (s.u.)
"I " '
II"*
n.4X
(
1"*.ct2-
"7, L "
DO (mg/L)
AI7O I
800 mg NaCI/L Conductivity.
- Temperature
" L
.O
' r -:r i iir 1 0...
pH (S.U.)
1000 mg NaCI/L DO (mg/L)
- s. iL.,
Conductivity I2q OI
(;tmhoslcm)
-120O
~-~0 Ziqc
- Temperature (oQ.
,WA
--*.I 2'~.E ~
u -
~
ii pH (S.U.)
"t.2Z 0 _.
'.IM I I-l 1 1 l
- .,q.*
- I ~
0 DO (mg/L)
UI 1200 mg NaCI/L Conductivity a')X IIA.aII
-I.
Z510
- Temperature (OC) pH (S.U.)
"1..
.90 DO (mg/L)19 1400 mg NaCl/L Conductivity (pimhos/cm)
~1i)-
- Temperature 4---. q bI.9 L'*A (09-..C V Initial Final Initial Final Initial I Final':,
- Temperatures performed at the time of test initiation, renewal or termination by the ai Information table located on Page 1. Alkalinity and hardness perfbnned by the analys analysis and transcribed to this bench sheet by:
for each age 105 of 105 SOP AT F
i4-.,
revision 06-01-11