ML12286A328
| ML12286A328 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 10/10/2012 |
| From: | John Carlin Tennessee Valley Authority |
| To: | Morgan C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, State of TN, Div of Water Pollution Control |
| References | |
| TN0026450 | |
| Download: ML12286A328 (108) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 October 10, 2012 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37219
Dear Ms. Morgan:
TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR SEPTEMBER 20"12 AND REVISED DMR FOR AUGUST 2012 Enclosed is the September 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. The toxicity report and revision to Discharge Number 101T, Biomonitoring for Outfall 101 for August 2012, are also attached. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.
I certify underpenalty of law that this document and all attachments were preparedunder my direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properlygather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gatheringthe information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significantpenalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sequoyah Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
PERMIIrEE NAME/ADDRESS (Include FacilityName/Location if Differentj- NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000
- I[NTEROFFICE OPS-5N-SQ.N TN0026450 t101 G F-FINAL SODDY - DAISY, TN 37384 PERMIT BEDISCHARGE NUMBE DIFFUSER DISCHARGE Facilj.:, TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATTN: Brad Love From 1 2-- 0 F YARm r I Mr 0 DAY To 1 12 109 1301 TI EAol MO I DAY NO DIS iSCHARGE NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING NO. FREQUENCY SAMPLE TYPE QUALITY OR CONCENTRATION FREQUENCY SAMPLE OF OF TYPE EX ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG. SAMPLE ** 41.3 04 0 30/30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 PERMIT *** Req. Mon. DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE 29.1 04 0 30/30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT 30.5 DEG. C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 3 04 0 30/30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S PERMIT -**- 3 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1761 03*
03
- 0 30/30 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE
- 0.017 0.032 19 0 24 / 30 GRAB MEASUREMENT 50060 1 0 PERMIT 0.1 0.1 MGIL FIVE PER CALCTD EFFLUENT GROSS MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0 62 0 30)30 CALCTD CHANGE MEASUREMENT 82234 1 0 PERMIT
- 2 DEG **wewe** ***,,* CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX CIHR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAM1_J'rITILE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Cadin properly gather and evaluate the information submitted. Based on my inquiry of the person or [ Pe persons who manage the system, or those persons directly responsible for gathering the President 423 8i-7 i n information, the information submitted is, to the best of my knowledge and belief, true, accurate. 423 843-7011 1 n
- .andcomplete. I am aware that there are significant penalties for submitting false information, SI E OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc. conc. was 0.057mg/L-limit 0.2mgIL) 2.
Biodetergent 73551 (max. calc. conc. was 0.038mg/L-limit 2.Omg/L) 3. Spectrus CT1300 (max. calc. conc. was 0.037mg/L-Iimit 0.050mg/L)
EPA Form 3320-1 (REV 3199) Pirevious editions may be used Page I of I
Mean # of Water Mean# of Water NOTES: %
Sample Date % Settlers Sample Date Asiatic Temp. ('C) LOCATION SUB LOCATION Gravid Asiatic COLLECTED BY CClamsm3 TClam 01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS 01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE 01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB 01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT 01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR 02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB 02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE 02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR 02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE 03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE 03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE 03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE 03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE 04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB 04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB 04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-1SV-24-1234 MJW May 2012 No Samples Collected June 2012 No Samples Collected July 2012 No Samples Collected August 2012 No Samples Collected September 2012 No Samples Collected
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Forrm Approved.
DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OM N+/-ame TVA - SEQUOYAH NUCLEAR PLANT BINo. 2040-0004 Address P.O. BOX 2000
-_ -_ (INTEROFCE OPS-5N-SQN.) TN0026450 F-FINAL SODDY - DAISY TN.37384 PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facil_ y_ A - SEQUOYAH NUCLEAR PLANT LoCation HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATTN: Brad Love Froml 1 109011 Tol11 09L L 30 NO DISCHARGE liZ]
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE Monitoring CERIODAPHNIA MEASUREMENT Not-Required 23 TRP3B 1 0 PERMIT *"***** eee*** 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE ** Monitoring 23 PIMEPHALES MEASUREMENT Not Required TRP6C 1 0 PERMIT 43.2
- PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEFTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my . *TELEPHONE DATE direction or superyision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather persons who and evaluate manage the information the system, submitted.
or those persons Based on my inquiry of the.person or directly responsible for gathering the President 43 8.-0! t infonnation the information submitted is, to the best of my knowledge and belief, true, accurate. 4.3 land complete. I am aware that there are significant penalties for submitting false information, SIGhTJp:F PRINCIPAL EXECUTIVE T including the possibility of fine and imprisonment for knowing violations. OFFICeR'OR AUTHORIZED AGENT AREA NUMBER MO DAY
__"c'_____________AUTHORIZEDAGET________R YEEARJ OIDý ITYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was not sampled in September 2012.
EPA Forim 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) 0MB No. 2040-0004 Name Address P.O. BOX 2000
.f.-.NTEROFFICE OPS-5N-SQON) TN024 1103 G F-FINAL SODDY -DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facilityy TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY m*IIIITnRINA PFRI"nn I EFFLUENT I YEAR Mo "D oEAR I DAY NO DISCHARGE [ *]*
ATTN: Brad Love From[ 12 0 I1 I To 1 12 09i 30 NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING I QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE SEX OF ANALYSIS TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE
- 7 9 12 0 19/30 GRAB MEASUREMENT 00400 1 0 PERMIT 6 .*9 SU THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED . SAMPLE '9 14 19 0 2/30 GRAB MEASUREMENT 00530 1 0 PERMIT MO30 AVG DAILY100 MX MGIL TMONTH TWICE/ GRAB EFFLUENT GROSS REQUIREMENT OIL AND GREASE SAMPLE <1 <1 19 0 2/30 GRAB MEASUREMENT 00556 1 0 PERMIT MO15 AVG 20 MX
'DAILY MG/L TWICE)
MONTH GRAB EFFLUENT GROSS REQUIREMENT FLOW, IN CONDUIT OR THRU SAMPLE 0.948 1.134 0 03 30)30 RcORDR MEASUREMENT TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD. SEE RCORDR EFFLUENT GROSS EFFLUENTAIGROSS REQUIREMENT MO AVG DAILY MX PERMIT.
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE lirction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the President Q Ok S .
- ic sit.
TYPED OR PRINTED P*ree;,de information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
a PRINCIPAL EXECUTIVE ICE -OR AUTHORIZED AGENT I
423 AREA CODE 843-7001 NUMBER 12 10
- 10 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all aftachments here) be used may be Page 1 of 1 EPA Foffn 3320-1 (REV 3320-1 EPA Form, (REV 3I9S) 3199) Previouseditions Previous editions may used Page 1 of 1
PERMII-TEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION. SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01)
Address P.O. BOX 2000 S*- J[NTEROFFICE OPS-5N-SQN) TN0026450 110 G F - FINAL SODDY - DAISY TN _37384___.. PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Fai_*,( TA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD
- I EFFLUENT I YEAR MO I DA YEAR IDAY NO DISCHARGE F]
ATTN: Brad Love From1 12 09L101 Tol 1[ 1j09 1j301 --NOTE:
NOTE:DCHRGe i Read inlstrJctionls before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 1 0 PERMIT * ****** REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT. DAILY MX UOUS TEMPERATURE, WATER DEG. SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 Z 0 PERMIT
- 30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 04 UPSTRM DEG.C MEASUREMENT - 04 00016 1 0 PERMIT 5 DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. MGD * *
- CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENTDAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 50060 1 0 PERMIT *0.1 0.1 MOIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT -MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE MEASUREMENT 04 CHANGE 82234 1 0 PERMIT 2 DEG C *********. ** CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my I/ TELEPHONE DATE dlirection or supervision in accordance with a system designed to assure that qualified personne John T. Carlin property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the . Vice President Seiuovah Site Vice President nformation, the information and amp;.;
awaw
.... submitted iht is ,significarn mhere are of my knowledge to the bestpenalties and belief, true, accurate, tor submitting false information, S-I---
E_ PN PAn -EECUTIVE E OFPRNCPA 423 843-7001 12 including the possibility of fine and imprisonment for knowing violations. O OR AUTHORIZED AGENT AREA NUMBER TY PRINTED OR I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of 1
PERMIIrEE NAMEIADDRESS (Include Facility Name/Location if Diffement) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000
--- LNTEROFFICE OPS-5N-SQ_.N) TN0026450 11 F - FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE R RECYCLED COOLING WATER Faciljiy TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON coUNTY.- MONITORING PERIOD EFFLUENT YEAR MO I DAY I YEAR MO DAY From J12 09 101 Tol 121091301 ** NO DISCHARGE [ý_ *]
ATTN: Brad Love NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING I QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I __ OF ANALYSIS TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT 23 CERIODAPHNIA TRP3B 1 0 0 PERMIT *******a 43.2
- PERCENT SEMI COMPOS REQUIREMENT EFFLUENT GROSS VALUE MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE 23 PIMEPHALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT *** 43.2
- PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER. I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision.in accordance with a system designed to assure that qualified personnel John T. Carlin persons gather property who and evaluate manage the information the system, submitted.
or those persons Based directly on my inquiry of the person or responsible for gathering the 7--sqo l*a" IteVice President S SieVc q o a Prsd n information, the information submitted is, to the best of my knowiledge and belief, true, accurate, LL "F J.. .. .423 843-7001 12 10 10 Sequoyah Site Vice President and complete. thathere a*re n hem t r" . to , , .fnSREoh d , ee IA s41 EX CU IV Iu41t,
--_.. , . ....... ..... (* II*~ Siu lR PRN includin th os including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEARMO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page I of I
PERMI-rEE NAME/ADDRESS (Include Facility NamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 F - FINAL
- - - (NTEROFFICE OPS-5N-SQN). TNO026450 118 G SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBERJ WASTEWATER & STORM WATER Facilijy TVA - SEQUOYAH NUCLEAR PLANT MONITORING PERIOD EFFLUENT Location HAMILT.ON COUNTY ATTN: Brad Love From 12T og Ioi I SYEAR I MO I DAY To To l 12A 109M I1301PAY NO DISCHARGE [ "*
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT **19 00300 1 0 PERMIT 2 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 PERMIT 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 25 00545 1 0 PERMIT *
- MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD "*ONCE/
. ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel 1 TELEPHONE DATE John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate.
sident AA-7flI n-_C'..!cm. ziwm t',
StVcPein ;ha-i atu signiiicant penalties for submitting false information, sIGNA'r-RE OF PRINCIPAL EXECUTIVE / , u ,IU including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT A NUMBER YEAR MO DAY TYPED OR PRINTED CO COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all aftachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMRJ N+/-ame TVA - SEQUOYAH NUCLEAR PLANT -- (SUBR 01) OMB No. 2040-0004 Adddress P.O. BOX 2000
-JLNTEROFFCE OPS-5N-SQN) TNO02645010T F - FINAL SODDY - DAISY, TN_373_84 F PERMIT NUMBýER PDISCHARGEI N'UMBER]
BIOMONITORING FOR OUTFALL 101 Fac.lli' TVA - SEQUOYAH NUCLEAR PLANT MONITORING PERIOD EFFLUENT L HAMILTON COUNTY
_ocr ATTN: Brad Love
ýYEAýMO Froml 12108 101 1 To1 12 1 08 1 31 DAY L RM NO DISCHARGE [LI]
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1I180 COMPOS CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 PERMIT *e****** -- ** 43.2
- PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT 43.2 '** PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER d- I Certify irection under penalty ofinlaw or supervision that this document accordance and all with a system attachments designed werethat to assure prepared under qualified my personnel I TELEPHONE DATE John T. Carlin properly gather and *evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the ice President Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. lam aware that there e sign..n"t fcf Subrinting iai-, informajion, including the possibility of fine and imprisonment for knowing violations.
SIGNAJIE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT 423 AREA 843-700i 1 NUMBER I i.
MO 4
DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
Toxicity sampling began on August 12 and ended on August 17. This is the revised August 2012 DMR data set for 101T. The toxicity report is attached.
EPA Form 3320-1 (REV 3199) Pmvious editions maybe used Page 1 of I
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: September 17, 2012
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 7. 1Q10: 3,.491
- 8. Outfall Tested: 101
- 9. Dates Sampled: August 12- 17, 2012
- 10. Average Flow on Days Sampled (MGD): 1741.838, 1748.488, 1747.571
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: August 14 - 21, 2012
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimrehalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint (%): Outfall 101: IC, 5 = 43.2%
- 17. Test Results: Outfall 101: Pitnephalespromelas: IC 25 > 100%
Ceriodaphniadubia: IC25 > 100%
UV treated Outfall 101: Pimephalespromelas: IC, 5 > 100%
Page 1 of 99
- 18. Facility
Contact:
Brad Love Phone #: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Outfall 101 samples collected August 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were
> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 2 of 99
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)
Sample Time (ET) Time (ET) Temp. TRC* Time (ET)
ID Collected Received (°C) (mg/L) Last Used By 101 08-12-12 0645 to 08-13-12 1540 0 .8 , 0 .7 t <0.10 08-14-12 1213 08-13-12 0545 08-15-12 1115 08-12-12 0705 to 08-14-12 1213 Intake 08-13-12 0605 08-13-12 1540 0.9 <0.10 08-13-12 0605 08-15-12 1115 101 08-14-12 0700 to 08-15-12 1553 1.9 1.9t <0.10 08-16-12 1114 08-14-12 0700to 08-16-12 1114 Intake 08-15-12 0620 08-15-12 1553 2.0 <0.10 08-16-12 1114 08-16-12 00to 08-18-12 1112 101 08-17-12 0600 08-17-12 1338 2 2., 2 .2 t <0.10 08-19-12 1115 08-17-12 0600 08-20-12 1114 08-16-12 0710to 08-18-12 1127 Intake 08-16-12 0715 08-17-12 1338 2.4 <0.10 08-19-12 1115 08-17-12 0615 08-20-12 1114
- TRC - Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 10 1 and intake were warmed to test temperature (25.0 +/- 1.0°C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page 3 of 99
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 20 - 20.22 hours2.546296e-4 days <br />0.00611 hours <br />3.637566e-5 weeks <br />8.371e-6 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101 08-14-12 1200 ET 08-14-12 1148 ET UV Treated Outfall 101 08-14-12 1213 ET
- 7. Test Termination: (Date/Time)
Outfall 101 08-21-12 1106 ET 08-21-12 1053 ET UV Treated Outfall 101 08-21-12 1122ET
- 8. Test Temperature: Outfall 101: Mean = 24.7°C Mean = 24.9°C (24.3 - 25.1 0 C) (24.6 - 25.3 0 C)
Test Temperature: UV-Treated Outfall 10 1: Mean = 24.8°C (24.2 - 25.1 0 C)
- 9. Physical / Chemic al Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
Page 4 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 14.- 21, 2012 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 1 4 [6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 95 Mean Dry Weight (mg)
Test Solutions (replicate number)
(%1Effluent) 1 2 J 3 4 I Mean Control 0.583 0.615 0.592 0.544 0.584 10.8% 0.535 0.669 0.686 0.649 0.635 21.6% 0.608 0.559 0.626 0.686 0.620 43.2% 0.582 0.563 0.487 0.614 0.562 86.4% 0.614 0.555 0.563 0.690 0.606 100.0% 0.532 0.673 0.506 0.571 0.571 Intake 0.537 0.569 0.551 0.687 0.586 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50 : TUc = 100/IC 25 Page 5 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 14 - 21, 2012 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions
(% Effluent) 6 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Test Soluetis Data (replicate number)
(% Effluent) 1 2 J3 ] 4 I 5 6 [ 7 1 8 [ 9 110 [Mean Control 30 33 29 33 31 28 34 32 30 32 31.2 10.8% 36 34 33 33 35 35 34 34 31 32 33.7 21.6% 36 31 33 31 30 37 35 33 32 30 32.8 43.2% 37 34 34 31 31 31 37 34 34 35 33.8 86.4% 34 34 36 34 35 33 34 35 36 37 34.8 100.0% 38 37 37 34 32 38 35 39 37 38 36.5 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC 25 Page 6 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 14 - 21, 2012 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 1 1 5 61 Effluent) 1 1(% 2 13 4 5 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(%Effluent) Data (replicate number) 1 2 3 4 [ 5 [ 6 7 8 9 10 Mean Control 30 34 34 31 32 29 32 31 29 31 31.3 Intake 33 29 33 33 33 36 33 32 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC 25 Page 7 of 99
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 14 - 21, 2012 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 ] 2 3 4 5 6 [ 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions(rpiaenm Mean Dry Weight (mg) )
(% Effuent)replicate number)
(%Effluent) 1 [ 2 3 ] 4 Mean Control 0.510 0.488 0.587 0.553 0.535 10.8% 0.673 0.559 0.631 0.594 0.614 21.6% 0.599 0.524 0.547 0.582 0.563 43.2% 0.592 0.516 0.548 0.588 0.561 86.4% 0.509 0.527 0.551 0.448 0.509 100.0% 0.550 0.565 0.526 0.494 0.534 Intake 0.62.1 0.573 0.647 0.482 0.581 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time 1Duration I Toxicant I Results (IC25) I Pimephalespromelas August 14 - 21, 2012 1225 KC1 0.81 g/L Ceriodaphniadubia August 07 - 14, 2012 1045 NaCl 1.08 g/L Page 8 of 99
PHYSICAL/CHEMICAL
SUMMARY
(0 Water Chemistry Mean Values and Ranges for Pitnephalespromelas and Ceriodaphniadubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 (D performed August 14-21, 2012.
(0 0
Test Sample ID Temperature (C) Dissolved Oxygen (mgfL) OH (S.U.) Conductance Alkalinity Hardness Total Residual 1%
to Initial Final Initial Final Initial Final (mtmhos/cm) (mg/L CaCO 3) (mg/L CaCO 3 ) Chlorine (mglL)
Control 24.8 24.7 7.7 7.9 7.55 7.50 311 62 89 24.7 - 24.9 24.6 - 25.1 7.6 - 7.9 7.8 - 8.0 7.43 - 7.67 7.36 - 7.59 302 - 323 62 - 62 88 - 92 24.8 24.6 7.9 7.8 7.52 7.46 291 10.8%
24.7 - 25.0 24.3 - 25.0 7.7 - 8.0 7.6 - 8.0 7.44 - 7.62 7.33 - 7.55 285 - 298 21.6% 24.8 24.6 7.9 7.8 7.54 7.44 278 24.7 - 25.0 24.4 - 24.9 7.7 - 8.0 7.6 - 8.0 7.44 - 7.62 7.33 - 7.55 272 - 283
- 4. 24.8 24.6 7.9 7.8 7.53 7.44 252 432 13 24.7 - 25.0 24.4 - 24.8 7.7 - 8.0 7.6 - 8.0 7.45 - 7.61 7.34 - 7.52 246 - 259 S 86.4% 24.8 24.6 7.9 7.8 7.51 7.42 198 24.7 - 25.0 24.3 - 24.8 7.6 - 8.0 7.6 - 7.9 7.45 - 7.60 7.31 - 7.52 192 - 205 100.0% 24.9 24.6 7.9 7.9 7.51 7.46 182 71 72 < 0. 10 24.8 - 25.0 24.4 - 24.8 7.7 - 8.1 7.7 - 8.1 7.42 - 7.60 7.32 - 7.57 177 - 186 70 - 72 71 - 74 <0.10- <0.10 Intake 24.9 24.6 8.0 8.0 7.51 7.45 182 69 73 < 0.10 24.7 - 25.0 24.4 - 24.7 7.7 - 8.2 7.9 - 8.0 7.43 - 7.61 7.33 - 7.54 176 - 186 68 - 70 72 - 74 <0.10- <0.10 Control 24.8 25.1 7.7 8.0 7.55 7.51 311 62 89 24.7 - 24.9 25.0 - 25.2 7.6 - 7.9 7.8 - 8.2 7.43 - 7.67 7.28 - 7.59 302 - 323 62 - 62 88 - 92 24.8 25.0 7.9 8.0 7.52 7.51 291 10.8%
24.6 - 25.0 24.8 - 25.2 7.7 - 8.0 7.8 - 8.2 7.44 - 7.62 7.29 - 7.59 285 - 298 2!1 24.8 25.0 7.9 8.0 7.54 7.52 278 24.7 - 25.0 24.8 - 25.1 7.7 - 8.0 7.8 - 8.2 7.44 - 7.62 7.30 - 7.60 272 - 283 x 4.%
43.2% 24.9 24.9 7.9 8.0 7.53 7.52 252 24.7 - 25.0 24.8 - 25.3 7.7 - 8.0 7.9 - 8.2 7.45 - 7.61 7.30 - 7.61 246 - 259
- 886.4% 24.9 25.0 7.9 8.0 7.51 7.51 198
___ 24.7 - 25.1 24.8 - 25.2 7.6 - 8.0 7.9 - 8.2 7.45 - 7.60 7.28 - 7.61 192 - 205 100.0% 25.0 24.9 7.9 8.0 7.51 7.52 182 71 72 < 0.10 24.8 - 25.1 24.8 - 25.2 7.7 - 8.1 7.9 - 8.3 7.42 - 7.60 7.29 - 7.63 177 - 186 70 - 72 71 - 74 <0.10- <0.10 Intake 25.0 24.9 8.0 8.1 7.51 7.52 182 69 73 < 0.10 24.8 - 25.1 24.7 - 25.1 7.7 - 8.2 8.0 - 8.2 7.43 - 7.61 7.30 - 7.64 176 - 186 68 - 70 72 - 74 <0.10- <0.10 Overall temperature (C) Average Minimum Maximum Pimephales promelas 24.7 24.3 25.1 Ceriodaphniadubia 24.9 24.6 25.3
PHYSICAIJCHEM ICAL SUM MARY Water Chemistry Mean Values and Ranges for PitnephalespromelasTest, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed August 14-21, 2012.
0,,
O Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness *Total Residual
-I' (0 Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO 3) (mg/L CaCO3 ) Chlorine (ng/L) 24.8 24.7 7.9 7.8 7.59 7.49 304 61 84 Control 24.8 - 24.9 24.5 - 24.9 7.8 - 8.1 7.6 - 7.9 7.50 - 7.65 7.34 - 7.61 298 - 316 61 - 62 84 - 84 24.7 7.9 7.8 7.60 7.48 291 10.8% 24.9 24.8 - 25.0 24.5 - 25.0 7.9 - 8.0 7.6 - 7.8 7.50 - 7.66 7.34 - 7.57 282 - 298 21.6% 24.9 24.7 7.9 7.8 7.59 7.48 279 21.6% 24.8 - 25.1 24.6 - 24.8 7.9 - 8.0 7.7 - 7.9 7.50 - 7.66 7.34 - 7.58 271 - 284 C, 43.2% 24.9 24.7 7.9 7.8 7.58 7.47 254
&1____ 24.8 - 25.1 24.6 - 24.8 7.9 - 8.0 7.7 - 7.9 7.49 - 7.66 7.33 - 7.58 246 - 258 24.7 8.0 7.8 7.56 7.46 203 86.4% 25.0 24.9 - 25.1 24.3 - 24.8 7.9 - 8.1 7.5 - 7.9 7.48 - 7.63 7.33 - 7.57 197 - 207 25.1 24.6 8.0 7.8 7.55 7.45 184 70 72 <0.10 100.0%
25.0 - 25.1 24.2 - 24.7 7.8 - 8.3 7.5 - 7.9 7.47 - 7.62 7.31 - 7.55 178 - 188 70 - 70 72 - 72 <0.10 - <0.10 25.0 24.6 8.1 7.9 7.54 7.46 184 70 72 < 0. 10 Intake 24.8 - 25.1 24.5 - 24.9 7.9 - 8.2 7.7 - 8.0 7.46 - 7.61 7.32 - 7.58 178 - 191 70 - 70 71 - 72 <0.10 - <0.10
- Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.8 24.2 25.1
SUMMARY
/CONCLUSIONS Outfall 101 samples collected August 12 - 17, 2012, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were
> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 11 of 99
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA-82 I -R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-82 l-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pinephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None Page 12 of 99
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage,. glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KC1 or NaCl.
Sodium Chloride (NaCl crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 99
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater. Organisms, EPA-82 l-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 2 1st Edition, 2005.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mgIL TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 - - - - - -
09/08/1998 0.015 - 0.014 0.005 - - 0.021 09/09/1998 0.003 - 0.031 0.011 - - -
09/10/1998 0.014 - 0.060 0.021 - -
09/11/1998 0.013 - 0.055 0.019 - -
09/12/1998 < 0.001 - 0.044 0.015 09/13/1998 < 0.001 - 0.044 0.015 09/14/1998 0.008 0.044 0.015 1 02/22/1999 < 0.001 - -
02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999 < 0.001 02/28/1999 < 0.001 - - I I 08/18/1999 - 0.015 0.069 0.024 0.006 08/19/1999 - 0.012 0.068 0.024 - -
08/20/1999 - 0.023 0.070 0.024 - 0.120 08/21/1999 - 0.022 0.068 0.024 - -
08/22/1999 - 0.022 0.068 0.024 - -
08/23/1999 - 0.025 0.068 0.024 0.006 -
08/24/1999 - 1 0.016 0.067 0.023 0.020 -
Page 16 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mgiL mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L. Quat TRC Azole 01/31/2000 -< 0.002 0.026 0.009 02/01/2000 - 0.011 0.026 0.028 -
02/02/2000 - 0.028 0.026 0.009 0.006 02/03/2000 - 0.008 0.027 0.009 - -
02/04/2000 - 0.006 0.027 0.009 0.005 0.109 02/05/2000 -< 0.002 0.027 0.009 - -
02/06/2000 - < 0.002 0.027 0.009 - I 07/26/2000 -< 0.0057 0.055 0.019 - -
07/27/2000 - 0.019 0.055 0.019 -
07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 07/29/2000 -< 0.0088 0.055 0.019 - -
07/30/2000 -< 0.0076 0.055 0.019 -
07/31/2000 -< 0.0152 0.055 0.019 0.006 08/01/2000 - < 0.0141 0.055 0.019 0.005 1 12/11/2000 - 0.0143 0.025 0.020 0.005 12/12/2000 - 0.0092 0.025 0.020 0.005 12/13/2000 -< 0.0120 0.025 0.020 - -
12/14/2000 -< 0.0087 0.025 0.020 - -
12/15/2000 - 0.0120 0.025 0.020 0.005 -
12/16/2000 - < 0.0036 0.025 0.020 - -
12/17/2000 -< 0.0036 0.025 0.020 - -
08/26/2001 - 0.017 0.06 0.021 0.006 -
08/27/2001 - <0.0096 0.06 0.021 0.005 - 0.021 08/28/2001 - <0.0085 0.06 0.021 - --
08/29/2001 - <0.0094 0.059 0.020 0.005 - 0.021 08/30/2001 - <0.0123 0.06 0.021 0.005 -
08/31/2001 - <0.005 0.059 0.020 - -
11/25/2001 - <0.0044 -- -
11/26/2001 - <0.0119 0.024 0.02 0.005 -
11/27/2001 - 0.0137 0.023 0.019 0.007 -
11/28/2001 - <0.0089 0.022 0.019 0.006 -
11/29/2001 - 0.0132 0.024 0.02 0.007 -
11/30/2001 - < 0.0043 0.024 0.02 I --
12/09/2001 - <0.0042 - -
12/10/2001 - <0.0042 12/11/2001 - <0.0104 - - -
12/12/2001 - 0.0128 0.024 0.02 0.008 -
12/13/2001 - <0.0088 0.024 0.02 - -
12/14/2001 - 0.0134 0.024 0.02 0.007 - 1 Page 17 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 -< 0.0079 0.023 0.02 0.006 01/03/2002 -< 0.0042 0.023 0.014 -
01/04/2002 - 0.0124 0.024 0.014 0.009 01/05/2002 -< 0.0042 - - -
01/06/2002 - < 0.0042 - - -
01/07/20021 - <'0.0089 0.024 0.014 0.006 1 02/24/2002 - < 0.004 - - -
02/25/2002 -< 0.004 0.023 0.023 -
02/26/2002 - 0.0143 0.023 0.023 0.007 02/27/2002 -< 0.0041 0.023 0.023 -
02/28/2002 -< 0.0041 0.024 0.008 03/01/2002 - < 0.0041 0.024 0.008 1 05/05/2002 - - - -
05/06/2002 - - 0.058 0.02 0.014 05/07/2002 - - 0.058 0.02 0.015 05/08/2002 - - 0.056 0.019 -
05/09/2002 - - 0.057 0.02 0.014 05/10/2002 - 0.056 0.019 I --
08/04/2002 - <0.0058 - - --
08/05/2002 - <0.0058 0.053 0.018 - 0.025 08/06/2002 - 0.0092 0.053 0.018 - -
08/07/2002 - <0.0107 0.055 0.019 0.007 08/08/2002 - <0.0061 0.055 0.019 -
08/09/2002 - 0.0152 0.054 0.018 0.008 1 10/06/2002 - <0.00497 - - -
10/07/2002 - 0.0153 0.054 0.018 0.009 10/08/2002 - <0.0092 0.054 0.018 0.007 10/09/2002 - 0.0124 0.053 0.018 0.009 10/10/2002 - 0.0134 0.054 0.018 0.009 10/11/2002 - <0.0042 0.054 0.018 -
01/12/2003 - <0.0035 - - -
01/13/2003 - <0.006 0.025 0.019 0.009 01/14/2003 - <0.01 18 0.026 0.020 01/15/2003 - <0.0063 0.026 0.020 0.009 01/16/2003 - <0.0034 0.026 0.020 01/17/20031 - <0.0034 0.026 0.009 -
04/06/2003 - <0.0073 - - -
04/07/2003 - <0.0189 - 0.021 -
04/08/2003 - <0.0117 - 0.021 -
04/09/2003 - <0.0139 - 0.021 0.016 04/10/2003 - <0.0113 - 0.021 0.018 04/11/2003 - <0.0073 - 0.022 - - -
Page 18 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 -< 0.0045 - - - -
06/16/2003 -< 0.0037 0.057 0.020 - - 0.022 06/17/2003 - < 0.0048 0.041 0.014 - - 0.024 06/18/2003 - < 0.0048 0.041 0.014 - - 0.024 06/19/2003 - < 0.0085 0.058 0.020 - - 0.025 06/20/2003 - < 0.0048 0.058 0.020 1 - 0.025 08/03/2003 - <0.0050 - - -
08/04/2003 - <0.0050 0.058 0.020 -
08/05/2003 - <0.0051 0.057 0.020 - 0.025 08/06/2003 - <0.0084 0.057 0.020 - 0.025 08/07/2003 - 0.0129 0.057 0.020 - 0.024 08/08/2003 - 0.0153 0.057 0.020 0.009 - -
10/05/2003 - <0.0043 0.057 0.020 - - -
10/06/2003 - <0.0043 0.057 0.020 - - 0.025 10/07/2003 - <0.0090 0.057 0.020 - - 0.025 10/08/2003 - <0.0106 0.057 0.020 - - 0.025 10/09/2003 - 0.0181 0.026 0.022 - - 0.025 10/10/2003 - 0.0183 0.026 0.024 0.009 -
02/01/2004 - 0.0093 0.027 0.009 -
02/02/2004 - <0.0034 0.026 0.009 02/03/2004 - <0.0034 0.026 0.009 -
02/04/2004 - 0.0124 0.026 0.009 0.009 02/05/2004 - <0.0034 0.026 0.009 - -
02/06/2004 - 0.0105 0.026 0.009 0.010 - -
05/04/2004 - <0.0123 0.026 0.019 - - 0.025 05/05/2004 - <0.0144 0.026 0.014 0.009 - 0.025 05/06/2004 - <0.0146 0.037 0.013 - - 0.025 05/07/2004 - 0.0227 0.058 0.020 0.009 - 0.025 05/08/2004 - 0.016 0.060 0.021 - - -
05/09/2004 - <0.0104 0.058 0.020 07/04/2004 - 0.0217 0.057 0.019 -
07/05/2004 - <0.0085 0.057 0.020 0.009 07/06/2004 - <0.0077 0.058 0.020 - - 0.031 07/07/2004 - 0.0252 0.056 0.019 - - 0.031 07/08/2004 0.0223 0.057 0.019 0.009 - -
07/09/2004 0.0182 0.057 0.020 0.009 -
Page 19 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco t-150M Hypochlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004 - <0.0187 0.000 0.014 - -
11/08/2004 - <0.0192 0.047 0.030 - - -
11109/2004 - <0.0233 0.048 0.016 - - 0.041 11/10/2004 - <0.0149 0.047 0.016 - - 0.041 11/11/2004 - <0.0149 0.049 0.017 -1 - 0 04 3 111/12/2004 - 1 <0.0253 0.048 1 0.017 0.042 02/06/2005 <0.0042 0.028 0.010 -
02/07/2005 - <0.0116 0.028 0.010 - 0.007 02/08/2005 - <0.0080 0.028 0.010 I 02/09/2005 - 0.0199 0.028 0.010 02/10/2005 - <0.0042 0.028 0.010 02/11/2005 - 0.0155 0.028 0.010 0.007 06/05/2005 -0.0063 06/06/2005 - 0.0043 - - 0.037 06/07/2005 - 0.0103 - - 0.037 06/08/2005 - 0.0295 - - 0.037 06/09/2005 - 0.0129 -
06/10/2005 - 0.0184 - -
07/17/2005 - 0.0109 0.026 0.009 -
07/18/2005 - 0.0150 0.026 0.009 0.036 07/19/2005 - 0.0163 0.026 0.009 - 0.036 07/20/2005 - 0.0209 0.026 0.009 - 0.014 0.036 07/21/2005 - 0.0242 0.026 0.009 -
07/22/2005 - 0.0238 0.054 0.018 - 0.014 10/30/2005 - 0.0068 - --
10/31/2005 - 0.0112 -- -
11/01/2005 - 0.0104 - - 0.035 11/02/2005 - 0.0104 - - 0.036 11/03/2005 - 0.0117 - - 0.036 11/04/2005 - 0.0165 - - 0.035 11/14/2005 - 0.0274 - - -
11/15/2005 - 0.0256 -
11/16/2005 - 0.0234 --
11/17/2005 - 0.0231 -
11/18/2005 - 0.0200 --
11/19/2005 - 0.0116 - - I - -
Page 20 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L Quat 101 mg/L mgiL TRC Phosphate Copolymer DMAD Azole Quat mg/L TRC EO/PO Phosphate 11/12/2006 - 0.0055 - - -
.11/13/2006 - 0.0068 -- 0.037 11/14/2006 - 0.0143 -- 0.037 11/15/2006 - 0.0068 -- 0.037 11/16/2006 - 0.0267 -- 0.037 11/17/2006 - 0.0222 -
11/26/2006 - 0.0188 -
11/27/2006 0.0138 -
11/28/2006 - 0.0120 -
11/29/2006 - 0.0288 -
11/30/2006 - 0.0376 -
12/01/2006 - 0.0187 - -
05/28/07 - - - - 0.015 05/29/07 - - - - 0.036 0.015 05/30/07 - 0.0084 - - 0.017 0.036 0.015 05/31/07 - 0.0103 - - - 0.036 0.015 06/01/07 - 0.0164 - - 0.017 0.036 0.015 06/02/07 - 0.0305 - - - - 0.015 12/02/07 - 0.0241 - - -
12/03/07 - 0.0128 - -
12/04/07 - 0.0238 - -
12/05/07 - 0.0158 - -
12/06/07 - 0.0162 - -
12/07/07 - 0.0175 --
04/13/08 - 0.0039 -
04/14/08 - 0.0124 -
04/15/08 - 0.0229 -
04/16/08 - 0.0143 -
04/17/08 - 0.0120 -
04/18/08 - 0.0149 -
10/26/08 - 0.0260 -
10/27/08 - 0.0151 - 0.017 -
10/28/08 - 0.0172 - - 0.041 -
10/29/08 - 0.0154 - 0.018 0.041 0.030 10/30/08 - - - - 0.041 0.030 10/31/08 - 0.0086 -- 0.041 0.030 Page 21 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L mg/L mg/L A-PF 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 - 0.0197 - - - - 0.017 - -
02/09/09 - 0.0237 - - - - 0.017 - -
02/10/09 - 0.0104 - - - 0.021 - -
02/11/09 - 0.0155 - - - 0.017 - -
02/12/09 - 0.0106 - - - 0.017 -
02/13/09 - - -
05/10/09 - 0.0129 - - -
05/11/09 - 0.0415 -- 0.0446 -
05/12/09 - 0.0053 - - 0.0396 -
05/13/09 - 0.0049 - - 0.0396 -
05/14/09 - <0.0141 - - 0.0397 -
05/15/09 - <0.0160 - - -
11/15/09 - 0.025 -- - -
11/16/09 - 0.0152 -- - -
11/17/09 - 0.0255 -- - -
11/18/09 - 0.0306 - -.
11/19/09 - 0.0204 - -- -
11/20/09 - 0.0093 - --
05/09/10 - 0.0192 - --
05/10/10 - 0.0055 - --
05/11/10 - 0.0100 - - - 0.039 -
05/12/10 - 0.0171 - - - 0.039 -
05/13/10 - 0.0041 - - - 0.039 -
05/14/10 - 0.0099 - - - 0.039 -
Page 22 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - August 17, 2012 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mgiL mg/L Quat mg/P, mgP mg/L Phos- meC EO/PO Quat Phos.- Phosphate TRC phate phate 10/31/10 11/01/10 0.0122 11/02/10 0.0112 11/03/10 0.0163 11/04/10 0.0107 11/05/10 0.0132 05/01/2011 - -
05/02/2011 - 0.04 05/03/2011 -- 0.04 05/04/2011 - 0.0155 0.04 0-05/05/2011 - 0.0179 - 0.04 05/06/2011 - 0.0089 - - -
11/06/2011 - 0.0168 11/07/20.11 - 0.0225 11/08/2011 - 0.0141 11/09/2011 - 0.0239 11/10/2011 - 0.0242 11/11/2011 - 0.0231 05/06/2012 - -
05/07/2012 -
05/08/2012 - - 0.041 05/09/2012 - 0.0145 - 0.041 05/10/2012 - 0.0298 -- 0.041 05/11/2012 - 0.0174 -
08/12/2012 - - - - 0.029 08/13/2012 - 0.0256 - 0.028 0.037 - 0.029 08/14/2012 - 0.0209 - - 0.037 - 0.029 08/15/2012 - 0.0279 - 0.028 - - 0.029 08/16/2012 - 0.0076 - - 0.029 08/17/2012 - 0.0446 - 0.032 Page 23 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 99
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of 'I U Rent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
lRkject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client I%. Number: N/A Asheville, NC Other (specify):
Facility Sampled: Sequoyah NP General Comments:
28801 Phone: 828-350-9364 roolý 5*fYo '-a ,P/1
-f .4+. 0 CCo, o, NPDES Number: TN0026450 Collected By: pw-k% 1 ej r - .Fax: 828-350-9368 Container .' .0.
Field Identification Grab/Comp. Collection Date/Time& Flow Rain Event?
Sample Description CVolume V. (MGD) as Appropriate Laboratory Use
'__ ___ Collected(
,_ R ... .~-. s nYes hT . ETg.Nr .A.rrival iP".Temp.
" . . By ..... T."ime " Appear-p rIf 8 "12 "t1-3 J- 2I
_______tt~~MW qi**. __ Inches Number . ':*.*
-:: Ii*:::::;:*: ,:*,:, ': .ance SQN-101-TOX Comp " Lj 2(2.5gal) 1'I/ A o2/1,oi17'_. _*-._ .
SQN-INT-TOX Comp *.x- Ot63 ' 1(2.5gal) 1 )2?ol;: -.! i i . 0Q Sample Custody - Fill In From Top Down . " se' iwpr-t,*'c. SAmAkes. ae.cewebl, wCturr Relinquished By (Signature): Date/Time Received By (Signature): Date/Time , WLC.Pt . & A
- 0I-I*o t'v -/// .o4:.0S 0 E Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at-6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page .Z MIS w
Whole Efflue' -)ample Receipt Log
ýIfl 1.11 *Sample temperature performed usingSample Receiving Thermometer. SN 6338 Date Time Received Received *Sa m ple' Project Sample 0
number number Sample name and description State Comments Received Received by from Temp. ( C) 08-13-12 1540 J. Sumner Sonic Delivery 0.8,0.7 8207 120813 .01 TVA/ SON Outfall 101 TN 08-13-12 1540 J. Sumner Sonic Deliver 0.9 8207 120813 .02 TVA / SQN Intake TN 0
(0 SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1.
2ent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Wlject Name? Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client RA). Number: N/A Asheville, NC Other (specify):
2880 General Comments:
Facility Sampled: Sequoyah NP 28801 Teneral Comments:
NPDES Number: TN0026450 Phone: 828-350-9364 ,, ,,ke a 00 ,Jo, Collected By: t, 0 fl"Ž*. Fax: 828-350-9368 Container " .. .. A ,.
FildIdntfiaton/
Field Identification Grab/Comp. Collection Date/Time Number & Flow Rain Event?
RainEent?
Sample Description Volume (MGD) aU)se .""aLabspratoo Collected (Mark as Appropriate)
.. ,. .. Start!~~ EndYes If Yes, No Trace ETS Log Arrival Tempp. By, Time* Appear-
- i*W ° * * ' t* ..? E ?YrJ.Str Inches _.._. Number, :'_""" ance SQN-101-TOX Comp 0700A 0. I" 2(2.5gal) Y/* [* 6" l 0 " - ! .
sQN-LNT-TOX Comp Wt d)o tv
__Sample o Uve" 1(2.5 gal)
Custody - Fill In From Top Down 4 uokmR s ,J -s r5 i.*. RAE Relinquished By (Signature): Date/Time Received By (Signature): Date/Time AtU ýt".eS.
-T A /03-e 6oC Skl._c ,hr C'r Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
CTlS Whole EfflueK _.,ample Receipt Log Page 12>
- Sample temperature performed using Sample Receiving Thermometer SN 6338
~Iiii Date Time Received Received by Received from
- Sample 0
Temp. ( C)
Project number Sample number Sample name and description State Comments (0 Received 08-15-12 0945 K.Keenan Fed - Ex 1.3 8215 120815 .01 CORR ERI - AAF McQuay International NC 08-15-12 0945 K.Keenan Fed - Ex 0.7 8216 120815 .02 Statesville Analytical - Cleveland WWTP NC 0 08-15-12 0945 K.Keenan Fed - Ex 5.5 8217 120815.03 United Water - Enfield WWTP NC 08-15-12 0945 K.Keenan Fed - Ex 5.5 8218 120815.04 United Water- Scotland Neck WWTP NC (0
(0 08-15-12 0945 . Keenan Fed - Ex 0.3 8219 120815 .05 Craven County Wood Energy NC 08-15-12 0945 K.Keenan Fed - Ex 0.7 8220 120815 .06 Duke Energy - McGuire NS - Outfall 002 NC 08-15-12 0945 K.Keenan Fed - Ex 0.6 8221 120815 .07 Progress Energy - Shearon Harris E& E NC 08-15-12 0945 K.Keenan Fed - Ex 0.5 8222 120815 .08 Carolina Beach WWTP NC 08-15-12 0945 K. Keenan UPS 2.0 8223 120815 .09 Morehead City WWTP NC 08-15-12 1553 J. Sumner TVA Courier 1.9/1.9 8207 120815 .10 WA - SQN - 101 TN 08-15-12 1553 J. Sumner TVA Courier 2.0 8207 120815 .11]TVA - SQN - Intake ITN I SOP G4 - Exhibit G4.2, revision 01-03-12
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1- of 4lent: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Wject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client Q*. Number: N/A Asheville, NC Other (specify):__________
cc General Comments:
Facility Sampled: Sequoyah NP 28801 Qt- 0,( &,a/ 7-V NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Iw.;'f r6 n3in.p Fax: 828-350-9368 NContainer um ber & Flow Rain Event?..:.... '.- 8.',O.1 Field Identification / Grab/Comp. Collection Date/Time VumeRain Event?.M.D.
Sample Description Volume (MGD) (Mark as Appropriate) LaboratoryUsc Collected*(Mark.as..ppr-p..iate. ::Laborator .Use
______________ tW-:- . " _____ Stets ..
Sta...End Start__ End_ _ *,t"_ *I ___._._ Yes Yes, Inches No No T Trace ETS Log .- Arrival "Number ".
Temp. By Time, Appearý-
ance .
SQN-101-TOX Comp 0 70o 67 Oo vr 2(2.5gal) / 1 SQN-INT-TOX Comp O,- ec 0 C/j*.E 1(2.5 gal) 2
- Sample Custody-Fill In From Top Down 4 CuV-abj Sf$t.5 1wrtA- SAKPLt' ft*1C.VCb Ji Relinquished By (Signature): Date/Time Received By (Signature): Date/Time .SAM-s4
- 46t S-Pv. 15e,- Date/Tim qr ..--..-
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block- Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 12S
-ýrs X
11--
Whole Efflue( '.ýample Receipt Log
- Sample temperature performed uslngSample Receiving Thermometer. SN 6338 Date Time Received Received *Sample Project Sample
~0 Received Received by from Temp. C0 C) number number 08-17-12 0950 K.Keenan Fed - Ex 0.6 8215 120817 .01 CORR ERI - AAF McQuay International NC 08-17-12 0950 K.Keenan Fed - Ex 0.6 8216 120817.02 Statesville Analytical - Cleveland WWTP NC 0 08-17-12 0950 K. Keenan Fed - Ex 2.0 8217 120817.03 United Water - Enfield WWTP NC 08-17-12 0950 K.Keenan Fed - Ex 2.0 8218 120817 .04 United Water - Scotland Neck WWTP NC CC 08-17-12 0950 K.Keenan Fed - Ex 0.7 8219 (0 120817 .05 Craven County Wood Energy NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .06 Duke Energy - McGuire NS - Upper Composite NC 08-17-12 1130 J. Sumner Murphy Courier 0.8 8226 120817 .07 Duke Energy - McGuire NS - Lower Composite NC 08-17-12 1338 J. Sumner TVA Courier 2.2/2.2 8207 120817 .08 TVA - SQN - Outfall 101 TN 08-17-12 1338 J. Sumner TVA Courier 2.4 8207 120817 .09 TVA - SON - Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12
- TS Page Iof 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: Tennessee Valley Authority County: Rhea Facility: Sequoyah Nuclear Plant Outfall: 101 NPDES #: TN0020168 Project #: A iJ1 Dilutionpreparationinformation: Comments:
Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 2500 2500 2500 2500 2500 TOW volume (mL)
Test organism information: Test information:
Organism age: 21) &ts O.z Randomizing template: JeLLOQ.
Date and times organisms Oa
- rh
" Incubator number and were born between: shelf location: __.,
Organism source: AYb)( O kP e- - Artemia CHM number: LIA 6S2 Drying informationfor weight determination:
Transfer bowl information: pH = -1. to 0 S.U. Date / Time in oven: 06o.. 1I%
0 Temperature = "S.*, C Initial oven temperature: lb, C Average transfer volume: Date / Time out of oven: ______
- 0. MLOck Final oven temperature: J '
Total-drying time: ".t*4 Dailyfeeding and renewalinformation:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 2 . -. *-L - I% O. 14-r 1R. 11-0w5______
W-____'_. MIS Oh s A, I% VLOI 11.01 1701.01l.2 O&F161 25 0l- _____$_5 " __ _\___ *l t*01l I .0 o-i'l1 5.0.16- _'m
__1*__
6 0g**121.'- g,-Ift 11.09 I 7.0,f n. do,
-- u-.4 -
a6--i7'IL Ito(,
Controlinformation: Acceptance -riteria Summary of test endpoints:
% Mortality: 07, < 20% 7-day LCsO > I W7.
Average weight per initial larvae: n3.SU W NOEC 1CCW7.
Average weight per surviving larvae: 0. Sq > 0.25mg/larvae . LOEC > 1007.
ChV >1/MO.
IC 25 I >1___T...
SOP AT20 -Exhibit AT20.3, revision 07-01-12 (I age 31 of 99
Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date: 0&. 1q.-I,."
Survival and Growth Data Day CONTROL 10.8% 21.6%
/A B C D E F G H I J K L 0 10DI(C/ 0 / /bo o'o10 lio o/0o'ro0
__01_ _ t(0 /0 tol/0 /0 .10 / /0 to(0 0 2 /0 to 10 lQ10 Ito (0 to t 10 to 10 (o SIQ0 10 10 10 to 10 10 2 /0 /0 10 10 /0 10 /0 /0 /0 /0 10 10 '0 'o /'0 /0 (0
4_______ 10 1o NO0 1 to Ito( 10 10 ) o 10 t0 to 6 /0 /0 t0 10 (0 10 I0 10 (0 /0 /6 ID 7 6 11) to t) /0Io t'io 1o 101 0 10o 1O fo /lb o t0C) 1to10 'ro 10 / CO A = Pan weight (mg)
Tray color code:: L.;!,%*ý ;t Analyst: wIl.y AA 13.-s 14.z4 13.Z4 14.10 13.B, I3.6 13-38 14.zs lZ.'e IS. 14.14 Date: OA.n-.t-z B = Pan + Larvae weight (mg)
Analyst: 3"Lt Lo*o %J.73 7.o.16 1I6V tq.q5 Zo.SS*$ Z.Sq 1q.17 z2*33 19Sq 11,93 "u.S-Date: 09-zl.17.
C = Larvae weight (mng) = B- A Hand calculated. S M 14-1. SAI 6. 6A0 .09 svi (".m. 6..&6 Analyst: _ k _III Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
eV 0% No 'I i'~p 4.
Analyst: __
- . - p- i - * . -- i -
Average weight per Percent reduction initial number of larvae (mg) from control (%) 0.614 0 . ts -&.&7. 0.ADULD - *.*a7.
I I --
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT20 - Exhibit AT20.3, revision 07-01-12 ge 32 of 99
I JETS Page 3 of 6 II Species: Pimephales promelas Client: TVA / Sequovah Nuclear Plant. Outfall 101. Non-treated Date: 9O&.Iq- I*.--
Survival and Growth Data Day 43.2% 86.4% 100%
M N 0 P Q R S T U V W X 0 0 to I oloto tol to to ( ~ IO U~1,C) to 1 O 10( to to 10 10/ /0 /(0 I0( I)10 0 /)
2 to to j0 10 10 to 10 1)0 to 0o 0 10 3 I) to00 I 1000 /0 /0to 0 00/0to I 4 t r/ I0 10 o 1 /0 t01 10o/ 0 /0(0 I 5 ID 1 10 10 /0 10 10 10 (0 10 (0 6 M0 1 O I
- J.
7
/) lb 10
( 1 1( ) b 10 tj ib Ib 0 101/
JO 0
(0 "0 10
'o0 to I A = Pan weight (nag)
Tray color code:: Li-;,,k Analyst: -l
- I hi.* 13.(.O I4.7 14.31 1 ml,.4 13.*)i , . o
'3.* 3. 10 13.3m i4so Date: _______ ,_____
B = Pan + Larvae weight (ing)
Analyst: 3Iq.a. tq.3 iq.3z ZoL&5 1S .'I 2 q.q z . t..,qz Il J' C 703r160 I W.zI V Date: oft.27.I-t I C = Larvae weight (mg) = B - A Anls:Hand Anal calculated.*t 5.Z7. S.I .tl'A1.14.. 6.1%; S.%S SAI* 6.1%0 S..S2 I.."S S.0'6 ,.'1%
p yst J+/-ý Weight per initial number of larvae (rag)
C /Initial number of larvae I
Hand calculated.4%1* 4N Analyst' . %Y l" .. 0 Average weight per Percent reduction initial number of from control (%) o. %L'.- ?.L1 0. O.'606
-S1. ".7.
larvae (Mg)
Comment codes: c = clear, d = dead, fg = fungus, k = kied, m = missing, sk sick, sm = unusually small, Ig= unusually large, dMr = decanted and returned, w =wounded.
Comments.
w I
SOP AT20 - Exhibit AT20.3, revision 07-01-12 Sage 33of 99
- ETS Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date:
Day 100% Intake Y Z AA BB 0 C0 Io to0 10 1 I0 (o to 10 2 1) 10 to 10 3 (Q '/0 /0 /0
/D1 /0,/0 5 /o0 /0 /0 t0 6 (o 0 10 (0 A =Pan weight (ng)
Tray color code:: V Analyst: M" '14.o 14.11 1'4.24 Date: *Of.t2.
B = Pan + Larvae weight (mg)
Analyst: M .2. Z0.47 T..7-L1.11 Date: oS-z7.. .*.
C = Larvae weight (mg) = B - A Hand calculated. S.S'" ',.i . .i h otj Analyst-Weight per Initial number of larvae (mg)
= C/ Initial number of larvae Hand calculated. "
Average weight per Percent reduction initial number of from control (%) 0 *-96 0A 7.
larvae (mg) __
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT20 - Exhibit AT2O.3, revision 07-01-12
- ge 34 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated August 14-21, 2012
,E PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Project n umber. 8207
,._ _ _ _ _ Not fnr Compliance Assessment. Interal Laboratoru OC Coeatratia(%) Replickte nlitnambered Flnatoumberftla,-ae APa welght(mg) BPaa+Larvae Larvaewetghtrt(m) Wdght /Surviitng Mea iget I Coeffilaetntuarraia Weight/ti-lai mahber MMaaadval Mea weightInlal Cliteataf P-er1a eed.dm f.
larvae wseight(.0g) -A-0 nberoftaa (rag) SvMngtumbero" tMa' ea alarva(rg) (*A) arao (5u..es 0a--re-rhlauea ooa rsI (%)
lourvartag) a~dktA (01g) aeao.irt A 10 10 14.47 20.30 5.83 0.583 0.583 Control aC 10 10 10 10 13.58 14.24 19.73 20.16 6.15 5.92 0.615 0.592 0.584 0.615 5.1 0.592 100.0 0.584 5.1 Not applicable D 10 10 13.24 18.68 5.44 0.544 0.544 E 10 10 14.10 19.45 5.35 0.535 0.535 1"% F 10 10 13.86 20.55 6.69 0.669 0.669 100.0 0.635 10.7 -8.8 G 10 10 13.68 20.54 6.86 0.686 0.686 H to 10 13.38 19.87 6.49 0.649 0.649 1 10 10 14.25 20.33 6.08 0.608 0.608 J 10 10 12.95 18.54 5.59 0.559 0.559 K 10 10 15.67 21.93 626 0.626 0.626 L 10 10 14.97 21.83 6.86 0.686 0.686 M 10 10 13.50 19.32 5.82 0.582 0.582 43.2% N o0 10 13.60 19.23 5.63 0.563 0.562 9.6 0-563 100.0 0.562 9.6 3.8 0 10 10 14.75 19.62 4.87 0.487 P 10 10 14.31 20.45 6.14 0.614 0.614 1 Q* 10 10 12.64 18.78 6.14 0.614 0.614 R 10 10 13.87 19.42 5.55 0.555 0.555 S 10 10 13.79 19.42 5.63 0.563 0.563 T 10 10 .14.52 21.42 6.90 0.690 0.690 U 10 10 13.50 18.82 5.32 0.532 0.532 V 10 10 13.70 20.43 6.73 0.6730.7 100% W 10 10 13.37 18.43 5.06 0.506 0.571 12.9 0100.0 0.71 12.9 2.2 X 10 10 14.50 20.21 5.71 0.571 0.571 Y 10 8 13.84 19.21 5.37 0.671 0.537 100%Intake Z 10 10 14.78 20.47 5.69 0.569 0.620 11.2 0.56995.0 06 1 .7 -0.4 AA 10 10 14.71 20.22 5.51 0.551 BB 10 10 14.24 21.11 1 6.87 0.6 060.687 Outfall 101; MSD = Minimum Significant Difference Duanett's MSD value: 0.0994 PMSD - Percent Minimum Significant Difference PMSD: 17.0 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
intake Dunnetas MSD.value: 0.0726 Lower PMSD bound determined by USEPA (10th percentile) = 128/6.
PMSD: 12.4 Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA. 2001a; USEPA, 2001b).
USEPA. 2001a. 2001 b. Final Report: Interlaboratoty Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity To. Me hn4,_ Vc zt
- I n. 2-Appemii. EPA-t2t-8-01-004 and EPA-821-B-01-005. US Enviromnental Protection Agency. Cincinnati, OR File: sqn101_081412data.xlsx Entered by. J. Sumner Reviewed by: G
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated aETS 6
August 14-21, 2012 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8114/2012 Test ID: PpFRCR Sample ID: VA I SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dlscharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Cbntrol 0.5830 0.6150 0.5920 0.5440 10.8 0.5350 0.6690 0.6860 0.8490 21.6 0.6080 0.5590 0.6260 0.6860 43.2 0.5820 0.5630 0.4870 0.6140 86.4 0.6140 0.5550 0.5630 0.6900 100 0.5320 0.6730 0.5060 0.5710 Intake 0.5370 0.5890 0.5510 0.6870 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5835 1.0000 0.5835 0.5440 0.6150 5.089 4 0.8127 1.0000 10.8 0.6348 1.0878 0.6348 0.5350 0.6860 10.744 4 -1.242 2.410 0.0994 0.6127 1.0000 21.8 0.6198 1.0621 0.6198 0.5590 0.6860 8.465 4 -0.879 2.410 0.0994 0.6127 1.0000 43.2 0.5615 0.9623 0.5615 0.4870 0.6140 9.607 4 0.533 2.410 0.0994 0.5835 0,9524 86.4 0.6055 1.0377 0.8055 0.5550 0.8900 10.256 4 -0.533 2.410 0.0994 0.5835 0.9524 100 0.5705 0.9777 0.5705 0.5080 0.6730 12.860 4 0.315 2.410 0.0994 0.5705 0.9312 Intake 0.5860 1.0043 0.5860 0.5370 0.6870 11.706 4 Auxiliary Tests Statistic Critical Skew Kurt Stlapiro-Witk's Test Indicates normal distribution (p > 0.01) 0.98195 0.884 0.03805 -0.4693 Bartletts Test Indicates equal variances (p = 0.82) 2.21405 15.0863 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 100 >100 1 0.09943 0.17041 0.00332 0.0034 0.4689 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95%CL(Exp) Skew IC05 87.934 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 lC25 >100 0.8 IC40 >100 IC50 >1000.7 0.6
, 0.4 0.2 0.1 0.0 - -
-0.2 0 50 100 150 Dose %
Dose-Response Plot UZ.5 0.7 0.8 0
0.5, 80.4
................. . ................. 1-tall, 0.05 level of significance 0*.3.
0.2 0.11 m i~4. 6 o - 0 S_
8 File: sqn101_08l412dt a.xlsx Entered by. J. Eumner Reviewed by: -V (tage 36 of 99
TVA / Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 Statistical Analyses ErrvtlronmentalTestlig Solutlonsý Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 811412012 Test ID: PpFRCR Sample ID: TVA / SQN 101 - Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.5830 0.6150 0.5920 0.5440 10.8 0.5350 0.6690 0.6860 0.6490 21.6 0.6080 0.5590 0.6260 0.6860 43.2 0.5820 0.5630 0.4870 0.6140 86.4 0.6140 0.5550 0.5630 0.6900 100 0.5320 0.6730 0.5060 0.5710 Intake 0.5370 0.5690 0.5510 0.6870 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5835 1.0000 0.5835 0.5440 0.6150 5.069 4 10.8 0.6348 1.0878 0.6348 0.5350 0.6860 10.744 4 21.6 0.6198 1.0621 0.6198 0.5590 0.6860 8.465 4 43.2 0.5615 0.9623 0.5615 0.4870 0.6140 9.607 4 86.4 0.6055 1.0377 0.6055 0.5550 0.6900 10.256 4 100 0.5705 0.9777 0.5705 0.5060 0.6730 12.860 4 Intake 0.5860 1.0043 0.5860 0.5370 0.6870 11.706 4 -0.067 1.943 0.0726 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88547 0.749 1.36005 1.98359 F-Test indicates equal variances (p = 0.20) 5.37752 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob dl' HomoscedastictTest indicates no significant differences 0.07258 0.12439 1.2E-05 0.00279 0.94881 1,6 Treatments vs D-Control Dose-Response Plot 0.8 0.7-0.6 0
0.5 --------------------- --------- ------------ .. ...... 1-tail, 0.05 level of significance 0.3 0.2 0.1 0O Nq N 8
File: sqn10I_081412dataxlsx Entered by: J. Sumner Reviewed by: I F (I age 37 of 99
Page 5 of 6 Species: Pimephalespromelas Date: ot'4,V*.-
Client: TVA / Sequovah Nuclear Plant Outfall 101. Non-treated Daily Chemnsfr:
r I I
I (Analyst
. d identified for -
Day each d. t'[performed PH D.O. and conducvit measurements only 1,D.O. and conductivity measurements onli I 2 Analyst MI 0 T =--4 Concentration [Parameter I nH(S.U.)
I DO (ma/L)
IConductivity CONonTR CONTROL Non-treated [ *Alkalinity (mg CaCO 3/L)
ZHardness em CaRCOZ.)'
- Temperature (0C) pH (S.U.)
DO (meL/ 1 0 10.8% Conductivity (inmhos/cm) q7 V14 S
- Temperature (M) U nH (S.U.) 7.C?-
VA.1%
"4!6 1
_LS.0 1 47 D~E I 7.Si II J DO (mF_/) -7.7 21.6% IS Conductivity (gmnhos/cm)
- Temnerature (*0)
_20 I IS I_*.
- II tuq.
I - -* ~ II I~ -I - - I -
pH (S.U.)
-- t
-741' 1.z II* - "-
i7.
1,. -.. So_ . 751l ,,
DO (mg/L) -'--I .1. ~ 9.01..
43.2% Conductivity . Ts (gmhos ) 255 7-55 U
- Temperature (0C)
U __________
"1,"1.1 I ...,.1tLI
a PH (S.U.) 7.31l 1.51 7.51 1 .9.0 86.4% Conductivity z6
&+/-mhos c) I6
%-b
- Temperature (00) "u4.'! H "LL.I.3h DH (S.U- "7.C5 -L- -1 I1
'V+I
-7.'IZ
- --- I. 4 Z.
DO (mFJL) 9.'
Conductivity (pinhos/c) ___
- Alkalinity 100% _10
- Hardness (mng CaCO3IL) i o.I'D
- TR chlorine (mg/L) 0O.1E
- Temperature (0C) T 0ISI II I r* -- !
PH (S.U.) I 7-9Z
-- Ik-----
DO (ma/)2 Conductivity (iimhos/cm) 100% *Alkalinity I St Intake (Ing 03 L)
- Hardness 1 (Ing Chlorine (m/L) '0.104
- TR chlorine (mg/L) 0. to
".Il t. I I *Te'm". "'
M U "S I " I° 5 II , U I Initial Final Initial Final Initial Final
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet SOP AT2O - Exhibit AT2O.3, revision 07.-01-12
ý7jage38 of 99
.j~.
ETS Page 6 of 6 Spec ies: Pimephalespromelas Cliei nt: TVA / Secuo ah Nuclear Plant. Outfall 101. Non-treated Date:
Dae c* t."i'A 1l ..
I i
Day (Analyst identified for each day. performed pHi,D.O. and conductivity measurements onlv., 7 I6 B
4 Analyst F-
.1 Concen- Parameter traon__
PH (S.U.) I 7.Co 5 I II q.*l'l II II DO (mg/L) ~1.~ I 1..' II II I Conductivity 0 CONTROL (iimhos/crn) 80 :31S "310 37.3
- Alkalinity Non-treated (Mg CaCO3/L)60
- Hardness
(-g CaCO3/L)
- Temperature (0 C) %A I -L*' I -,/Ia.
I. I. 71 IP- - I ~** 41. I ~*-. ~
pH (S.U.) /;iJS*'l" 7.41 -7 St -7. 45 7-51 "7..S 1. QS 7.33 DO (mg/L) - '6'0____
Conductivity 2`65a.. us I
10.8% Z%5*7t
- Temperature (*C) 'MA *.1. t IM- !6 14.1) "I, k '1%. 2
, i PH au.)
DOnutagv T i .---- ..
-7.
-7.57.
-7
. p 1.33 1 . SL-7.i
. .,7 I 21.6% 7.9 -
Conductivity z st I 43.2%
i pH (S.U.)
DO (mnA/L' Conductivity (gmhos/cm i' "7.£1. 7.~l V4m
-7.51 -7.SL I ".C; 7.7.5.
'.z":4
- Temperature 4-nH (S.U.) 1 -7.4 DO (mF/L) ~
0-O ~. ~I77 ~ *. 11.c I I
86.4% Conductivity O~mhos/cm) 6"
-7.S 7-4.3
- 6 S
- TemperatuJre C*C) i U - "141.94.
DR (S.U.) .aQQ
-7C '7_441 .M -797 DO (mR/L) 21 II i
Conductivity l7'~
- Alkalinity 100% 'Vt-I (mg CaCO 3IL)
- Hardness (Mg CaCO3/L)
- TR chlorine (mg/L)
-wI
~O. /0
- Temperature (*C)
-U I 2*.D Conductivity 100% *Alkalinity i Intake
- Hardness
(*ag CaCO3/L)
- TR chlorine (mg/&)
.. .a.1 "IW -*-a. V.I.I F15I I I*4t.-.
nritial1 . -. I .I IF Finial Iitl Final I Initial Final InI6tial
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet SOP AT20 - Exhibit AT20.3, revision 07-01-12 i age 39 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated August 14-21, 2012 PimephalespromelasChronic Whole Effluent Toxicity Test (D* EPA-821-R-02-013, Method 1000.0 0O SEiTS b EnvironmentalTestig Solution,' Inc.
Daily Chemical Analyses Project number: 8207 Concentration IParameter I .~ I -- - I Day 6 Initial F Inal Initial I Final Initial I Final Init fl I Vi...l I pH (SU) 7.49 7.49 7.60 745 7.67 75 DO (mg/L) 7.6F 7.8 7.6 78 7.6 7.
Conductivity (imhos/cm 302 ,315 310 Control Alkalinity (mg/L CaCO 3) 62 Hardness (mg/L CaCO 3) WE Ah 88W
______Temperature (C) 24.7 2446 2509 24.7 24.81 2467 2498 2497 24.8 24.6 24.7 2024.8 24.31 PH(SU) 7.62 7.44 7.44 7472 7.5 7.50 7.59 7.43 7.52 7335 7.5215 7.55 7.33 DO (mX/L) 7.7 79 8.0 7.8 4724 7.9 789 27 790 28 7.6 27 7.9 4 768 4 2780 21 787.8 247 2.8.0 210.6% enpraur(~)74(jLmhos/cm)
Conductivity
_______ 297__ 246 29 294___ 294__ 285__ 285__ 285_298 H(SU) 762 744 744 752 745 752 757 432 5 4 5 5 .5 73
______Temperature (CC) 24.7 24.4 25.0 2467 24.8 24.6 24.9 24.9 24.8 2456 24.7 2480 24.9 24.4 pH (SU) 7.61 7.42 7.45 7507 7.51 7.50 7.59 7.42, 7.51, 7.37 7.51 7.52 7.56 7.34 4.% DO (mxnL) 7.7 78.0 9 8.0 7.8 7.9 7.6. 7.9 7.6 8.0 7.8 7.8 8.0 2.% Conductivity (gmhos/cm) 280__ 283__280 279 278 276 282 Temperature CC) 24.7 24.4 25.0 24.6 24.8 24.8 24.9 24.6 24.8 24.5 24.71 24.8 24.9 24.4 pH (SU) 7.60 7392 7.45 7.51 7.51 7.48 7.58 7.42 7.451 7.34 7451 7.52 7.53 7.,314 86O4% DO( L) 767 7.8 8.0 7.9 8.0 7.8 8.0 7.6 7.8 7.7 8.0 7.8 7.8 7.9 4.% Conductivity (IRmhos/cni) 254_255 195 2492 24 241~6 2I5~
_______Temperature CC) 24.7 24.43 25.0 _ 24.6 24.8 24.6 24.9 24.6 24.8 24.7 24.7 2468 24.9 24.5 piH (SUY) 7.60 7.42 7.42 7.54 7.53 7.49 7.57 7.49 7.44 7.38 7.44 7.57 7.54 7.32 DO (mg[L) 7.7 7.8 8.0 8.1 8.1 7.9 8.0 7.7 7.8 7.7 8.0 78. 7.8 7.9 8.% Conductivity (,Imhos/cm) 183 183 186 181 1796 177 183
_______Temperature (OC) 24.8 24.4 25.01 24.7 25.0 24.6 25.0 24.8, 2. 24. 7 24. 8 24.7 24.9 24.5 HIE (SU) 7.61. 7.42 7.44 -7.50 7.52 7.50 7.58 7.4 7.43 7.39 7.434.4 75 7.33.
DO (mg/L) 7.7 79 80 8.0 8.2 8.0 8.10. 7.9 7.9 8.0 8.7. .0 C'onductivity (jnhos/cm) 183 186 185 kJd 182 181 17678 100%Itk Alkalinity (mgtL CaCO3) 6826 70 7 Hardness (mg/L CaCO 3) 72 74 72 Total Residual Chlorine (niL <0.10 .<0.10 <0.10 ITemperature CC) 2. 24. 4 24.9 24.7 25.0 24.6 25.0 2. 25.0 24.7847 2. 24.9 24.7 File: sqn101_081412chem.xls Entered by: J. Sumnner Reviewed by: A
- Page 1 of 7 OETS Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia Client: Tennessee Valley Authority County: Hamilton Facility: Sequoyah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project #: &,Ldl Dilutionpreparationinformation: Comments:
Dilution prep (%) 10.8 21.6 43.2 86.4 I100 Eftluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 3406 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organismsource information: Test information:
Oranism age: < 24-hours old Randomizing template color. 6'0 $.b Date and times organisms were born ol't " i2..O- 04o0 lb C Incubator number and shelf between: location: 7-6a-Culture board: a2It r. locaton Replicate mnuber 1 2 3 4 1S- 6 17 8 9 10 Culture board cup number [. I -W I U M Mt 1.U YWT1batch:
Transfer vessel information: PH = -611 S.U. Temperature = L C Seleasm batch:
Average transfer volume (mL): .S 6. 05 Daily renewal information:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake termination time ot.N-r 0 IN? O -I %2- t .rLo041 tOO it .Q_.
1
___ ~ ~ ~
_________ 1S 2ri~r I2.oels.0% Xtt~
2 o1.n..0' O40 oIq'. 1T-tjOtt. IU t lo t. I It Os.lo. I"L IS Itt 17_.oeI1.QJ I" S 1.
4 _____. _
doI1.11. iOS"_ 1_9 'L_ a. % 091t-l.ot L. 601 11-__I,-
6 I.
S-20.r01 A~l IL. I 1'P l1% Rs 1--61.)I ni 7 Of" Lt-1-2
- h I Controlinformation:
- Summary of test endpoinils:
Control-1 Control-2 Acceptance criteria
% of Male Adults: 07- 07, < 20% 7-day LC5s >00Q1.
% Adults having 3'(Broods: 1001 fool, >
Ž80% NOEC 1001,,
% Mortality: 17. 07. < 20% LOEC >1861.
Mean Offspring/Female: 31.7. 1 ,31.3 15.0 offspring/female ChV > 10.
,% CV: (,'A"27. I S.6#7. < 40.0 % IIC25 ') 100 1J.
SOP ATI I - Exhibit AT 11.2, revision 07-01-12
( age 41 of 99
- ETS Page 2 of 7 Species: Ceriodaphnliadubta Client: TVA / Seauovah Nuclear Plant, Outfall 101 Date: __ _ __ _ -I_ _
CONTROL-1 Surv.ival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 110 1 Young produced 0 C) CD 0 0 1 Adult mortality \- k.. %- %-.
I 2 Young produced Adult mortality J l
... [
0 1 1
le 1
_ I- I- -
3 4
_ Young produced Adultmortality Young produced I
C.,
1
_ -1
,[ O__
0 I -
U II - f C--
Adult mortality [ U
- 5 Young produced *. _ %0i 1 I .
Final Adult Mortali ty _ _.- t.. I .
Xfor3 Broodsu _ 1 0- 0 0 0 0 O I Note: MeAdult morta litveD = dead), SB = epit brood (single brood between two %plit days), CO cariy oere
- (offcpnng earied over with adult durig trasfer).
tenOfping/emale: _',L CONg: 10.8% Survival and Reproduction Data Day No te:ng mortalit(L=
produce rdueAdult fiv. ead B spli D"- 1
, bro _______
snl ro pltbtentody), CO 2 3 4
- carr overnumber________
Replicate (f(n care vrwt dl uigtase) 6 7 8 ___
9 0 1JYoung prd__
- __[* * *O * * * *_. .
Adult mortality _ _
- 21[Young produced 0 0 0I QI j CL 10 C JAdult mortality VwV *IL.tL _UJ . .-j.._ ... 1L *U ] ... .
2 4
_IAdult J Youn Young produced Adult mortality Adult mortality produced Young mortality produced I 0N
.rai
-C]~3
.. jZ[__
C)6 "_
__ 0t Lj\-I 1
~C 1.
a.i O..c.
M_
I.
U 0C 0
I0 C_
0 6 Young produced .c.10 m F ni 0) 0 .0
__fAdult nortality .I 2 L- . I~P1_~~~H L- tL4k Adult___ mo talityt.. t 4 Young produced -6 tre T It4 I Totalyoung produced
- __ 3S JS I. IL Final Adult Mortality .U- *. T U.. - _ _
FinotAdult Mortality \ C - - -eCo L.-o Qr *-
Note:Mdullmortality (L - live.D - dead), SB-eplit brood (single brood split betwoeentwo days),CO-cam over (offispijer caruied over with adult during rasfr Concentration: i
%Mortality:
Mean Offspring/Female: S'1
% Reduction from Control-1: -. O7 SOP ATI I - Exhibit ATI 1.2, revision 07-01-12 I age 42 of 99
Page 3 of 7 0ETS Species: Ceriodaphniadubia Client: TVA / Sequovah Nuclear Plant. Outfall 101 Date: ______I__"__L_
CONC: 21.6% Suri' ival and ReproductionData Replicate number Day 1 2 3 4 5! 6 7 8 9 10 1 Youngproduced 0 C_ 1f 0 0 1 0) 0_
2 JAdult Young produced 3J*Young produced mortality W
0 0 U . .L.__
0____
0 0 0 Adult mortality I I L-I'.--1 4 Young produced SAdult mortality Yonpone I *'-
REI .S I
S
' I___*- - ___
S.
w I t'-
a I_'l [ &
I I
5 Youngproduced I I . [
Adult mortality __ - - - -I' 'I UI' 6 Youug produced 1 1C) 0 0 C) I cc > 7_..
Adult mortality I U _
7] Young produced ~~' 40 1-15 '
Total young produced -' *
- I "
- 3 ' I I ? -
Final Adult Mortalit .- . - -
Note: Adult mortality (L li D -dead), SB -split brood (sge brood split between two days), CO ca over (offsorimitcarried over with adult durinatranafer).
Concentration:
%Mortality: 07.
Mean Offspring/Female: ,32, 1
%Reduction from Control-1: ". l7.
CONw: 43.2% Survival and ReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 produce edYoung ) ( * ( 0:
].Adult mortality [12. 7&7 1
. U. L.. I ... _ L.. =. LL.. .
Adult mortal'yit - ---
2 Young produced I C .. 1 5 1 0 1 . 1 Q 2 4 on mortality Young rdcdAdult I1 produced I L ,. L. I_ --. I t._ () t I I.. I CI 4 Adult mortality Young produced SC) L 10 kA S1 6...oJ~ 1 So.I 4C CzS~.. l
__[Adult mortality C_~C...I L I .... ' '- '
4 Yung produced %I L Adult mortality L . L __ UU.
6
- Young produced 1 ) I .. C)
Adult mortality ___- . - . -. - L- 1 A-f /
7] Young produced l 1 1 Total young produced
-.b Iq-&- 1311 31 a% 1 :31 13'4 13" 1 as k_ L.. *- L_ K_ I C_ I_ . L_
Notef
% _- .4 - UIUSI.ffWUPt JUIUY. - SUU)J.
_/ -A - an - k flIý
- i V~
O Ot OU.'. H I Concentration: -1
%Mortality:
Mean Offspring/Female: 3.
% Reduction from Control-I: -* 7.
SOP ATI I - Exhibit ATI 1.2, revision 07-01-12 (Iage 43 of 99
S a
0
- ETS 0 Page 4 of 7 Species: Ceriodaphniadubia Client: TVA / Seauoyah Nuclear Plant Outfall 101 Date: _ 0'1 1+_17._
CONC: 86.4% Surv,ival andReproductionData 1 Replicate number Day I Young produced 1 C
2 3 4
)0 T 6 C
17 18 9
) C 10
_J1 Adult mortality Fk 4_ 0 L, LJ L_ L* [
Youngproduced A1 , r.. 0,"I 0 Q I 0 Q, Adult modaity =T
\._I \_, T. °I =
3 EYoung produced ,1 Adult mortalityI 01 D.QI .. .....Q. JL..
c>. o I_ ,_ ,
4 1 Young produced A!i- 7 I Young produced Adult mortality
_L i,_,,\I _ 1" ____ 'J I'-1 I--I N" I" I
'L
%. 1
.utmortality 6 Young produced C)
I Adult mortality i [I' I1 I° a -I I-7 Youngproduced IS %4% 14 lt0 %
Total young produced "AS4 6 ""S .-,* . X1 Final Adult Mortality --1 \-
'- \_ '- C I Note: Adult mortality (L - live, D- dead), SB split brood (singlebrood split between two days), CO - can over (offspri carried over with adult dourimtransfer.
Concentration:
%Mortality: 7.
Mean Offspring/Female:
% Reduction from Control-1: -I . S7.
i CONC:
Day 2
3 100%
Young produced1 Adult mortality Young produced Adult mortality AYunglproduiedt 1 L3 1
1-2 C) 2
_[0 [
3
_ I' L_ I.'_'
C)
Survival andReproduction Data
_1 4
Replicate number '_"
5 10 I L I 01 [
6 L
c))
7 I -L_
o 1 0- 1 8 9 L- IL--
- 1 10
- 4. Adult mortality I_
Young produced Adult mortaliyI I V[ II- '1 1 L_
'- JI L_ _l
- I*- __I 5 IYoungproducedI- I I VWI Cm J1 ('
Adult mortality I 1_x-I x---I .-- I l I_ -ILI -
6 Young produced Adult mortality l I_
[
L k 0_0__ o0 L-
_i 0 .
I-----
7] Young produced iI I 4e 1i 1 9, 19 1 "7 -1 .O it 19 Total young produced U T31 3-1 3 .3 ,8 36 YI 31 Z 8 Final Adult Mortality "C.._ -c. N. - L_ -L_ C. -. C .*__
AW*s:Adult mortality (L i- live, D - dead), Sn - split brood (single brood split between two days). CO = car over (offssrint carred over with adult durint transer).
Concentration: i
% Mortality:
Mean Offspring/Female: -
% Reduction from Control- 1: 1.0Z SOP ATI 1 - Exhibit ATI 1.2, revision 07-01-12
- age 44 of 99
- ETS Page 5 of 7 6 tsawl=*M U~m*TstfDfm Species: Cerlodaphniadubia Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date: O.t'it.
CONTROL-2 Survival andReproduction Data Replicate number Day- I Young produced 1 2 3 4 5 6 7 8 9 110 2 -Yugpodcd _ 0 0___ C) C) C 2 Young prdue Adult mortality . 1010_oi,..C.[o L..J- \L .\J_ j.__ 0.1.. ..
4 3_____ d*t produced Young
~F 1= r_ 0 _ L Adult mortality L_ - - L I i- I -.I 5 Young produced %*IC) '-- I___
10 10 I'*3 1
_ A dult mortality I~ %. L - _j. . L_~ -- %[..
6 [Young produced 0 T01 0 1 Adult mortality J__
7 Youngproduced Total young produced .* "' * : A".". * * '
Final Adult Mortality X for 3Y Broods JC:
y.
I '-
Y.
5 .2-.
5Z 5Z
-- I Note: Adult mortality (L - live, D dead). SB = split brood (single brood split between two days), CO - carry over (offspring carried over with adult durin transfer).
I Ccent.ration:
_Mortal__:
_I IMean Offspring/Female:
CONC: 100% Intake Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced C) 1Q5 1I _ (.2 0 0L "
I Adultmortality L.
2 Young produced 0 0 Adult mortality *).. - I _ '[- 1 Lc _ L.
3 Young mrai produced O p _OI 1 O cI.
4 IAdult mortality -,Ij_,,.. L I kI I'.
_. L_
4 Young produced L I I1 SI S1i[S ISI I I Adolt mortality Q . - L IL - - -
6 Young* produced [
A__dolt mortality [ [~I~*I- 1 . T! -W
-I'_~i' I.. I* _=IL.
61 Young produced [C)j1c01TOK1 0 1 ZII0iC A__dult mortality ______ .~L L 7 Young produced Total young produced
[6 .~ IS .. 10~ 1-1 31 3 ac3 3-6 "3, 32. 1 31- 'Aq Final Adult Mortality L \_ .. LZ = -C7 ý Nvote:Adult mortality (L -=lte. D dud), Sa - spit brood (tingle blood split betweentwo days), )c- an over t(oftain carred over with adult duttna transfer).
Concentration:
% Mortality: 07.
Mean Offspring/Female:
% Reduction from Control-2: - I SOP AT1I1 - Exhibit AT 11.2, revision 07-01-12 I age 45 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated August 14-21, 2012 (ia! 0:T Verification of Ceriodaphnia Reproduction Totals (0
a E&trn*"nsrtTeUng SIWUmInc,. c Control-1 86.4%
(0 Replicate number Total Day Re plicate number Total D 1 2 03 40 60 7 0 9 10 Day 1 2 3 4 _5 6 7 8 9 10 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0- 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 .0 0 0 0 0 0 0 0 0 4 5 4 4 5 5 5 5 6 4 6 49 4 5 7 5 5 4 5 6 5 6 6 54 5 111 11 10 "12 10 9 12 11 11 11 108 S 13 12 12 11 12 12 14 1L2 13 13 124 0 0M 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0
L67 014 0 18 0 15 160 0 16 14 17 15 15 15 155 7 16 15 19 18 19 16 14 18 17 18 170 Total 30 33 233 3 31 28 34 32 30 32 312 Total 34 34 36 34 35 33 34 35 36 37 348 100%
10.8%
DyRe plicate number Total Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 T 1 2 3 4 05 6 70 0 9 10 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 00 00 00 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 L5 6 4 34 6 6 4 L6 5 35 51 1 13 12 11 12 12 10 1 12 12 12 1 19 3 0 0 0 0 0 0 0 0 0 0 0 7 18 16 18 17 17 19 17 16 14 15 ,167 7 1818 19 16 15+19 17 20 18 19 179 Total 36 34 33 31 30 37 34 33 32 30 337 Total 38 37 37 34 32 38 35 39 37 38 365 21.6% 3 0 0 0 0 0 0 0 0 0 0' 0 1 Control-2 Day Dy 1 0 2 000~Re 3 4 0 o0o0o plicate 6 170 18 5 numberToa 19 0 110 Toa0 TalRe D3icate number Total S1 2_ 3 4 5 16 7 8 19 1 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 0 .0- 0 0 0 0 0 50 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 5 6 5 5 4 4 4 48 4 4 5- 5 5 6 4 4 4 4 4 45 5 13 !11 12 10 10 13 13 13 11 13 119 5 10 12 13 12 10 10 10 13 11 12 113 6 10 10 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0
7 118 1 15 F6 16 14 19 17 1-6 1-7 1 6 7 16 17 16 114 16 15 118 14 14 15 155 Total 136 131 33 131 30 137 _L5 _L3 32 130 1328 Total 30 34 134 131 132 129 132 131 129 131 313A" 43.2% 100% Intake DyRe plicate number Total DyRepDlicate number Total Ia 2_ _ 4 5 6 7 18 19 1101 S 1 0 0 0 0- 0 0 0 0 0 10 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0L 0 0 0 0 0 0 0 0 0 3 0 0- 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 11 50 -4 6 4 6 5 5 7 5 5 4 6 53 7 1 14 85 5 13 12 12 3
12 4 5 1 11 6 12 11 12 12 121 5 11 12 12 r13 10 14 12 12 13 11 120 64" 10 20 0 0 0 0 0 0 0 0 0 0 I0 0 0 0 0 0 0. 0 0 6 0 7 18 17 17 15 15 15 19 15 18 18 6 7 1_6 p C-*15 1 1US 1*15 M16S 1s19 1 17 1 159 Tot-s-,
M 1 541M iM13131 371= 31133s 413r] Total 1I3 3 313 213 41 33 File: sqnlO1_O81412data.xlsx Entered by. J. Sumner Reviewed by V
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated August 14-21, 2012 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 0
Quality Control Environmental Testing Solutions, Inc.
Verification of Data Entry, Calculations, and Statistical Analyses Project number: 8207 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%)
(%) 1 2 3 4 Control - 1 30 33 29 33 31 28 34 32 30 32 100 31.2 6.2 Not applicable 10.8% 36 34 33 33 35 35 34 34 31 32 100 33.7 4.4 -8.0 21.6% 36 31 33 31 30 37 35 33 32 30 100 32.8 7.6 -5.1 43.2% 37 34 34 31 31 31 37 34 34 35 100 33.8 6.7 -8.3 86.4% 34 34 36 34 35 33 34 35 36 37 100 34.8 3.5 -11.5 100% 38 37 37 34 32 38 35 39 37 38 100 36.5 6.0 -17.0 Control - 2 30 34 34 31 32 29 32 31 29 31 100 31.3 5.6 Not applicable 100% Intake 33 29 33 33 33 36 33 32 36 34 100 33.2 6.0 -6.1 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 2.021 PMSD = Percent Minimum Significant Difference PMSD: 6.5 PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Intake:
Dunnett's MSD value: 1.459 Lower PMSD bound determined by USEPA (10 percentile) = 13%.
PMSD: 4.7 Upper PMSD bound determined by USEPA (90"h percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OIL File: sqn101_081412data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Surpner Reviewed by: 7
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated August 14-21, 2012
- ET Statistical Analyses Ceriodaphnla Survival and Reproduction Test-Reproduction Start Date: 811412012 Test ID: CdFRCR Sample ID: TVA / SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnla dubia Comments: Non-treated Conc-% 1 2 3 4 6 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CVQ/% N Sum Critical Mean N-Mean Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 33.800 1.0000 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 140.00 75.00 33.800 1.0000 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 122.00 75.00 33.800 1.0000 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 136.50 75.00 33.800 1.0000 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 151.00 75.00 33.800 1.0000 100 36.500 1.1861 36.500 32.000 39.000 5.954 10 150.50 75.00 33.800 1.0000 Intake 33.200 1.0807 33.200 29.000 36.000 5.991 10 Auxillary Tests Statistic Critical Skew Kurt Kolmogorov D Test Indicates non-normal distribution (p <= 0.05) 0.98421 0.895 -0.0927 -0.4597 Bartiletts Test indicates equal variances (p = 0.35) 6.58769 15.0863 The control means are not significantly different (p = 0.91) 0.12078 2.10092 Hypothesis Test (1-tall 0.06) NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs Control-1 Linear Interpolation (200 Resamples)
Point % SD 96% CL Skew ICO5 >100 IC10 >100 IC15 >100 1.0 iC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 0.5 c0.4 10.3 0.2 0.1 0.0
-0.1 - -
-0.2
-0.31 0 50 100 150 Dose %
Dose-Response Plot 45..
40 35 330 S25.
S20
= 15, 10l 5.
u . .
..- N, Cil C; o~ - Co g 8 File zqn101 081412dmetxte Entered by. , SJ ter (tage 48 of 99 Reviewed by. -- F
TVA / Sequoyah Nuclear Plant, Outfall 101
. Non-treated August 14-21, 2012 0.:T Statistical Analyses SEnvinonn,.ntal TentlfgSolutions.
Ins.
Analysis used for PMSD calculation only.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/14/2012 Test ID: CdFRCR Sample ID: TVA/SQN 101 End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 6 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21.6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43.2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86.4 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-1 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 -2.828 *2.287 2.021 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 -1.810 2.287 2.021 43.2 33:800 1.0799 33.800 31.000 37.000 6.660 10 -2.941 2.287 2.021 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 -4.072 2.287 2.021 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 -5.995 2.287 2.021 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.05) 0.98421 0.895 -0.0927 -0.4597 Bartlett's Test indicates equal variances (p = 0.35) 5.58769 15.0863 The control means are not significantly different (p = 0.91) 0.12078 2.10092 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 2.02145 0.06479 32.12 3.90741 7.9E-06 5, 54 Treatments vs Control-1 File: sqnlOl 081412data.:dsx Entered by: J. Sumpner I age 49 of 99 Reviewed by: --*7
TVA / Sequoyah Nuclear Plant, Intake Non-treated August 14-21, 2012 a
ETS Start Date: 8/14/2012 Test ID: CdFRCR Statistical Analyses
- Ceriodaphnia Survival and Reproduction Test-Reproduction Sample ID: TVA / SQN 101 - Intake End Date: 8/2112012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 Control-1 30.000 33.000 29.000 33.000 31.000 28.000 34.000 32.000 30.000 32.000 Control-2 30.000 34.000 34.000 31.000 32.000 29.000 32.000 31.000 29.000 31.000 10.8 36.000 34.000 33.000 33.000 35.000 35.000 34.000 34.000 31.000 32.000 21,6 36.000 31.000 33.000 31.000 30.000 37.000 35.000 33.000 32.000 30.000 43,2 37.000 34.000 34.000 31.000 31.000 31.000 37.000 34.000 34.000 35.000 86A 34.000 34.000 36.000 34.000 35.000 33.000 34.000 35.000 36.000 37.000 100 38.000 37.000 37.000 34.000 32.000 38.000 35.000 39.000 37.000 38.000 Intake 33.000 29.000 33.000 33.000 33.000 36.000 33.000 32.000 36.000 34.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 31.200 0.9968 31.200 28.000 34.000 6.193 10 Control-2 31.300 1.0000 31.300 29.000 34.000 5.645 10 10.8 33.700 1.0767 33.700 31.000 36.000 4.435 10 21.6 32.800 1.0479 32.800 30.000 37.000 7.578 10 43.2 33.800 1.0799 33.800 31.000 37.000 6.660 10 86.4 34.800 1.1118 34.800 33.000 37.000 3.532 10 100 36.500 1.1661 36.500 32.000 39.000 5.954 10 Intake 33.200 1.0607 33.200 29.000 36.000 5.991 10 -2.258 1.734 1.459 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.91798 0.905 -0.1715 0.33113 F-Test indicates equalvariances (p.= 0.73) 1.2669 6.54109 The control means are not significantly different (p = 0.91) 0.12078 2.10092 Hypothesis Test (1-tall, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.45886 0.04661 18.05 3.53889 0.03657 1,118 Treatments vs Control-2 Dose-Response Plot
'1" 40 35 30 4
.. 1-tail, 0.05 level of significance 25 L20 15 10 5
.0 C? 2 C14 COe4
.G=
J9 8
File: sqnl 01_0814 2data.xlsx Entered by: J. Sumnner
- age 50 of 99 Reviewed by: -
- ETS 6E~M-MITngSwmonth.,
Page 6of 7 Species: Ceriodaphniadubia Date: 0 1 t-I+t ..-
Client: TVA / Seauoyah Nuclear Plant, Outfall 101 Dailv Chemistr:
I.
Day (Analyst identified for each de ',performed pH D.O. and conductivity measurements only.)
0 1 I 2 Analyst Ij~ ITS ~ SV Concentration jParameter pH (S.U.)
_ w
-?.CC II 7. s 7.143 11 51 1 '7.55 I DO (mg/L)
CONTROL Conductivity (ginhos/cm)
- Alkalinity (mg CaCr 3/L)
- Hardness I '36C 313 1 (Mng CaCO3/L) 'a.
at. .0 S
- Temperature (QC)
U DH (S.U.) -Y.CIL 07.q4 0 .4 7508 ( E S ~46 .
DO (me*J -, "7 .II -- 7 10.8% Conductivity (gimhos/cm)
- TemDerature (00) a~
M-4.1 .1 0,L. It a - I--*- II - I - II.
.pH (S.U.) ?7.C= -7.qi B 2S7, S17.Si DO (mfiL) -7.17 1 73 21.6% Conductivity (iunhos/cm)I
- Temperature (PC)
S.
-Loa
-LA-1 _15.
SW~
oH (S.U.) -1. '7.4 9
- a -~-------4I - - - L i-.-.-..--,
DO (mulL) 7.7 43.2% Conductivitk f Sq 9.s; 1-55 (Aihmos/cm) ZL
- Temnerature (C* IlA-1 V.
]
.4 -
- 14- -1 1- L--
pH (S.U.) 7.60 -7~
7.46 S DO~g/) ____ 7A' 7.__
86.4% Conductivity
________ Tempoerature C0C) IN t pH (S.U.) -7.qq 7.47- -1.C3 1.0, 100%
DO (mnwU Conductivity (Iunhos/cm)
- Alkalinity (Mg CaCO 3/L)
I T
.*Hardness (MigCaCO 3/Q)1*.
choie
________ m1 0.1M
- Temperature (00)
PH (S.U."
DO im 7.1 (pinhos/cin) UIS 100% *Alkalinity Intake mg
- Hardness
- TR chlorine (mg/L) 0 .00
- Temperature CC) 2S.0 . . 15.1 R".I 1.t Initial Final Initial Final Initial Final
'Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: __
R.C.wby g~0 1 K-rn SOP ATI I - Exhibit ATi 1.2, revision 07-01-12 ypge 51 of 99
- ETS Page 7 of 7
~* Species: Ceriodaphniadubia
- A*I:"*
.'.*" Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date: O0.IL 1...
I Dae Ot L I
V Day (Analyst identified for each day. performed pHi D.O. and conductivity measurements only.) I Analyst I Yvj,,-.11 S Ic31 1143 f3Z.
Concen- Parameter
. tration
-I PH (S.U.)
DO (mg/L)
Conductivity 7A2 315 (umhos/cmr) 310 -sZ3 CONTROL *Alkalinityt (mg CaCO 3/L)
- Hardness (mg CaCO3 /L)____ 7AM
- Temperature (QC) 1Lý t
- pm
.7 DH (S.U.) 'JA~.
I:2e m
10.8% Conductivity 71S65_ __
(iimhoslcm) v -. r
- TemDerature (T) -- A .Ve L.4~ I "..m..t PH (s.u.) 7.O7.S? -7.94 -7.5Z _____ ____
DO (mg/L) 7A Ts C 21.6% Conductivity -2 (pmhos/cmn) Z
______*Temperature (IC) ir3, 'L0 LAtk s I0 ~-u..-Lf L.
PH (s.u.) , 67.51 .S'- 7.1 7 7.SC 73 DO mglL) 7! 7 .0 7. V 43.2% Conductivity (grnhos/cmn) MCA V40 7,4
_____*Temperature (C) 1.' it~ . -1 . g *z tC 24 vH (S.U.) '4.F*1-7.41 1 Z-19 11-7.S3 1 -1.4Li 1 7.SS. A 7.93 V -7.22 DO (mg/L) 86.4% 13.01F i
Conductivity (gmnhos/cm)
- TeM "erature"S (C -
07 1 th 7-4 pH (S.U.) 12q' -1.6 76-54
-7~ t7Z DO (mc/L) I.
Conductivity 00% *Alkalinity I I.
- Hardness
- TR chlorine (mg/L)
- Temperature ('C)
I a PH (S.U.)
DO (migUL a-Conductivity I 100%
Intake (gmhos/mn)
- Alkalinlty (mg CaCO3QL
- ardness (Mg CaCO0/L)
- TR chlorine (mg/L)
- Temperature (QC a _ ntil Fna niil Final IInitial Final IInitial 11Final m*
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
'-"4 *SOP ATI 1 - Exhibit ATi11.2, revision 07-01-12
'-'4-'?
r__ ge 52 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated August 14-21, 2012 "ET S
- 0EPA-821-R-02-013, Ceriodaphniadubia Chronic Whole Effluent Toxicity Test Method 1002.0 (1 0 0@
Daily Chemical Analyses to (a0EnTs Environmental Testing Solutions. Inc. Project number: 8207 Concentration IParameter Da 0 Da7 Day2 Da 3
.9 -
Initial _7 Final IInitial I Final IInitial_ 2 Final 1Initial Ia oH (SU) r DO (mffIL)
Conductivity (pnhoslcm) I . 310 Control Alkalinity (maLL CaCO)
- 9--
62 Hardness (mg/L CaCO3) 88 Temperature (C) 24.71 25.11 24.91 25.0 24.9~.9 25.21 24.91 25.2 9 - 9*- 9~ -, ~9 .9- -- 9- - I - ~9 - I -
pH (SU)-- 7.621 7.441 7.441 ' 7.58 7.451 7.591 7.571 7.58 I .. J A A DO Ima/l,* 7-7 7-K K'O KO 70 N11 Nf0 *70 10.8% DO Arm-/T I 77 78 80 so 79 81 80 79 Conductivity (ILmhos/cm) 2970M 294 1_*Mff= 294 L%ý ý 285 2 85 Temnerature (0I0 24.71 24.91 24.91 25.2 24.91 25.01 25_oi 25.11 24R *11 25 24~ 94121 9421 250 pH (SU) 7.62 7.43 7.44 7.59 7.51 7.59 7.59 7.60 7.52 7.54 7.52 7.56 7.55 7.30 21.6% DO(mg/L) 77 78 80 81 79 80 79 79 79 81 80 79 7.8 8.2 Conductivity (pnhos/cm) 280 283 4 280 273 272 273 ? _____28 Temperature(70 247 249 249 250 249 250 250 251 248 250 247 248 24.8 25.0 pH(SUJ) 7.61 7.44 7.45 7.61 7.51 7.59 7.59 7.58 7.51 7.54 7.51 7.56 7.56. 7.30 4.2% DO(mgL) 7.7 7.9 8.0 8.1. 8.0 8.0 7.9 7.9 7.9 8.1 8.0 7.9 7.8 8.2 Conductivity (pmhos/cm) 254 r 255 @ W 255 249 9 248 7r , 246 259 Temperature CC) 247 24.8 25.0 24.9 25.0 25.0 25.0 24.8 24.8 24.8 24.7 24.8 24.8 25.3 PH (SU) 7.60 7.43 7.45 7.61 7.51 7.58 7.58 7.61 7.45 7.53 7.45 7.55 7.53 7.28 8.% DO (mg/L) 7.6 7.9 8.0 8.2 8.0 .8.0 8.0 7.9 7.8 8.1 8.0 7.9 7.8 8.2 Conductivity (pnhos/em) 203 203 194 192 196 196 205 Temperature (oC) 248 25.0 2.0 24.9 25.0 25.1 25.1 251 24.8 24.8 247 249 24.8 25.2 pH (SU) !7.60 7.44 7.42 7.63 7.53 7.57 7.57 7.61 7.44 7.54 7.44 7.55 7.54 7.29 DO (mz/L) 7.7 7.9 806 8.2 8.1 7.9 8.0 7.9 7.8 8.1 8.0 7.9 7.8 8.3 Conductivity (wnhos/cm) 183 183 186 181 179 183 100% Alkalinity (mg/L CaCO3 ) 72 Hardness (mg/L CaCO3 ) 72 71 74 Total Residual Chlorine U m<0.10 <0.10 <0.10 Temperature (00 24.9 25.0 25.0 24.9 25.0 24.9 25.1 24.8 25.0 24.8 24.8 25.0 24.9 25.2 pH (SU) 7.61 7.45 7.44 7.64 7.52 7.57 7.58 7.58 7.43 7.56 7.43 7.56 7.54 - 7.30 DO (mg/L) 7.7 8.11 8.0 8.2 8.2 8.0 8.1 8.0 7.9 8.2 8.0 8.0 7.8. 8.2 Conductivity (pmhos/cm) 183 .. 186 . 185 182 . 181 176 1% 184 100% Intake Alkalinity (mg/L CaCO3) 68 687 Hardness (mg/L CaCO3 ) 72 74 72!
I jKesidual Unlorine jmtL) < 0A0 <0.10 . . . l <0.101 Temperature (C) 25.0 24.91 25.01 25.11 251 24.9 2. 24.71 24.91 24.7 24. 24.7 24.94 25.1 File: sqn101_081412chem.xls Entered by: J. Sumner Reviewed by: 4
- ETS a~-~
Page 1 of 6 I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
I Client: Tennessee Valley Authority Species: Pimephalespromelas County: Rhea Ii Facility: Sequoyah Nuclear Plant NPDES #: TN0020168 Project #: g14"I Outfall: 101 I Dilutionpreparationinformation:
Dilution prep (%/) 10.8 21.6 43.2 86.4 100 Comments.
Each concentration was UV-treated for 2 minutes to remove pathogenic I
Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Interferences.
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:
Organism age: -fz 0, oL.oLb Randomizing template: t Date and times organisms 01 t I l- . 1 C) Incubator number and were born between: shelf location: _ _ _
Organism source: ATby,. 60 ., oC 1i3--L pTCI Artemia CHM number: f.1 -
Drying informationfor weight determination:
Transfer bowl information: pH= 'Wo.tO S.U. Date / Time in oven: n- t'.oo vt',
0 Temperature = "l.. C Initial oven temperature: 10C Average transfer volume: Date / Time out of oven: o0"J.. t*,
- 0' ""'"~ Final oven temperature: "
Total drying time: -1A 46 Dailyfeeding and renewal informiation:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake' 0 O-15.101-1StL co I's%%4 %r. L ilt9.01 to L0IS.I %-..%IL 3 61.11.1-1. co-t) t'tQ I %UlsI?-~t. 16 k'Loftm. IIL 0-1+1E]
4 af-kt" 1L- o*1t .%<, iq% .L . ll .
- 170011. 0e 1208T1+/-a1.0 '
5 tA d1Si 9 11 A II2091-.O0 I ti20ai.ol 6
i OU'1-nan 71 Controlinformation: Acceptance criteria Summary of test endpoints:
% Mortality: -<20% 7-day LCso ) I w7.
Average weight per initial larvae: . NOEC 1W 7.
Average weight per surviving larvae: O. S l >0.25mglarvae LOEC ItOol.
ChV 1*0 1067.
IC 2 5 ( 5 1007.
SOP AT2O - Exhibit AT20.3, revision 07-01-12
- age 54 of 99
Page 2 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfail 101, UV-treated Date: o atwi-IM-Survival and Growth Data Day CONTROL 10.8% 21.6%
,_A B C D E F G H I J K L 01 ) It to to it) 0 t/C0t I t OC to) to 0 1 10 o 3 U 10 1O /0 /0 10 /01 t0 2 I0 10 o to 10 110 /0 /0 /)010 3 /0b 0103 to 0 /0 10 10 10 IC 10 4 1C) IQ C 10 (0/o 10/0 toIC' to0 . lo
/0 1 o /0 /0_i /00IC 1c M Q 6 10 101(0 to/0 /0 (0 /0 10 (0 '0 /0 7 IlV
/* 10 0 to, 3 10 0 0 to /0' (0 A = Pan weight (mg)
Tray color code::
Analyst: 146 1--.. Ass 1-3 3. t4.4z 4m.v-8 14.7%{, .4.l4. orp Is.
Date: 08.06.1t-B = Pan + Larvae weight (mg)
Analyst:. I".i6 1`. aq.zs tl.36 1 .Z 2Loo06 2.o.21 Zas57 IS.1 dt Z0.34 l.qs q.5$5 1-113 Date: op.z.7. -I C - Larvae weight (mg) = B - A Hand calculated. S tO S A 'sC
(-1 S.S 6,O .'b% Is N4 A s.'Il S.aIi
. 0-Analyst: ,_ ___
Weight per initial number of larvae (mg) I
= C / Initial number of larvae Hand calculated.
Analyst:
.1 y
N 49 $
0*
- 0
'9 0*
'0 0'
4
- U- - U- - U- *-- U-Average weight per Percent reduction initial number of from control (0le) . o.6'!S - tqA7.
a i -I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.
SOP AT20 - Exhibit AT20.3, revision 07-01-12 (Iage 55 of 99
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant Outfall 101, UV-treated Date: OI.i'A-VL.
Survival and Growth Data Day 43.2% 86.4% 100%
,_M N 0 P Q R S T U V W, X 0 0Q 1D Ito Iri to to 10 C) t) to /0 2 00 10 10 (C 10 (0 10 to i0 to1 101
[0 to I_Q10 0) IO 10 1 to0 1O to00 3 I to I to 10O ( to / 010 I /0 /0 / ) /0 4 I) /0 10 /0
/C) / 0ItI 10 /0 /0 IC) )/I 5 /0 /0 10 /0 10 /0 /0 1,A /01 /0 /0 6 to to 110 11 to0t to to /0 O1t 0 7
7__ (o 10 t0 t1) I*01 1 /0 /b /6Q A = Pan weight (mg)
Tray color code::
Analyst- _ InL -L&,L 1l.- I.' 13.*
34D .14 135.77' 15.A 13-1&* 14.8z ,4.3s IL-.3 13.Uf ILI.
Date: _ _ _n_..__-
B = Pan + Larvae weight (mg)
Analyst: _ -MR _ _q.47
_._6
___._ 7-_.oo 1L63 1_0I I41.041 18_.36 tq___ 16.1_ IS - 17._7_
Date: o8.zI7 C = Larvae weight (mg) = B - A SA.'L S~iI.6 1 S.t t t S.d% S.A- S..z% 4.1t S S60 7 SL.6 2.J, .sc'i Hand calculated.
Analyst:
Weight per initial number of larvae (mg)
C/Inltial 1 number of larvae Hand calculated. i , V,%* e Analyst. C - o" v" 0" U" LAverage weight per Percent reduction initial number of from control (%) o--.)7.
.¢OP. q..&T. 0, S .- ).
larvae (mg Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT20 - Exhibit AT2O.3, revision 07-01-12 (f age 56 of 99
Page 4 of 6 Species: Pimephalespromelas Client: TVA / Seciuovah Nuclear Plant. Outfall 101. UV-treated M I I I Date: O*.L Day 100% Intake Y Z AA BB 0 10 to10 (
S/00 2 to ot 3 Ib t o t o t 4 to
/0 (0 10 5 1 _)/0 t 0 6 (0 (0 t0 10 7 / i
____ __ _ 10 (0 to, A = Pan weight (71g*
Tray color code:: 4 Analyst: lit. i.1 Date: O_.___. _ _
B = Pan + Larvae weight (nag)
Analyst- J-r 2 Z .3 "
Date: 0O.z7.1z C = Larvae weight (mg) = B - A Hand calculated. (o . L' L Analyst-Weight per initial number of larvae (mg)
= C./ Initial number of larvae Hand calculated.
Average weight per I Percent reduction initial number of from control larvae (mig)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm.= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments.
SOP AT20 - Exhibit AT20.3, revision 07-01-12.
(Iage 57 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated August 14-21, 2012 (D
PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Project number. 8207 Not for Compliance Assessment, Internal Lablratory QC Conenatratio() Repfle letlalauamberf mIal-mber-aF an. A=P-uw eght(mg) B-Paa+Lar-se Larewelght(mg) Weight/SonIhag Me-aweight/ Coeffictafvarlatlon Weight/lldtd lnumber Meanerrival Merwreghtl/talt CeltTdeletof Peent reductonfmthan larvae weightnOg) -A-B mumberaftervue(mg) Surtvirgotreof ateklrw -b oPSot tae(eag) (0) humberoflarvee varitdion g e0ovtn (00) t-r) m5.r ... (%) (ea 00 ee ra=i,,.)
A 10 10 12.88 17.98 5.10 0.510 0.510 B 10 10 14.40 19.28 4.88 0.488 0.488 Control C 10 10 15.49 21.36 5.87 0.587 0.535 8.3 0.587 100.0 0.53S 8.3 Not applicable D 10 10 14.63 20.16 5.53 0.553 0.553 E 10 10 13.33 20.06 6.73 0.673 0.673 10.8% F 0 10 14.62 20.21 5.59 0.559 0.614 8.0 0.559 100.0 0.614 8.0 -14.9 G 10 10 14.26 20.57 6.31 0.631 0.631 H 10 10 12.84 18.78 5.94 0.594 0.594 1 10 10 14.35 20.34 5.99 0.599 0.599 2 10 10 13.19 18.43 5.24 0.524 0.524 K 10 10 14.06 19.53 5.47 0.547 6.0.547 L 10 10 13.11 18.93 5.82 0.582 0.582 M 10 10 14.08 20.00 5.92 0.592 0.592 432% N 0 10 O1 10 13.47 35 18.63 5.16 0.516 0.516 0 10 10 13.53 90 19.01 .80580.561 5.48 0.548 6.4 058100.0 0.548 0.561 6.4 -5.0 P 10 10 13.17 19.05 5.88 0.588 0.588 Q 10 10 13.27 18.36 5.09 0.509 0.509 86.4% R 10 10 13.69 18.96 5.27 0.527 0.521 4.5 0.527 975 0.509 &.6 4.8 S 10 10 13.96 19.47 5.51 0.551 0.551 T 10 9 14.82 19.30 4.48 0.498 0.448 U 10 10 14.33 19.83 5.50 0.550 0.550 V 10 10 12.73 18.38 5.65 0.565 0.565 100% V ot 27 83 .50550.534 5.8 O5 100.0 0-534 5.8 0.1 W 10 10 13.24 18.50 5.26 0.526 0.526 X 10 10 12.76 17.70 4.94 0.494 0.494 Y 10 10 15.22 21.43 6.21 0.621 0.621 100% Intake Z 0 10 14.61 20.34 5.73 0.573 0.573 100.0 0.581 12.5 -8.7 AA 110 14.37 20.84 6.47 0.647 0.647 BB 10 10 14.39 19.21 4.82 0.482 10.482 Outfafl 101: MSD = Minimum Significant Difference Dunnett's MSD value: 0.0685 PMSD = Percent Mirinium Significant Difference PMSD: 12.8 PMSD is a mensum of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Intake:
Dunuett's MSD value: 0.0826 Lower PMSD bound deter-mined by USEPA (I 0th percentile) = 12%.
PMSD: 15.5 Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interiaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b).
USEPA. 2001a. 2001b. Final Report: Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, O1.
File: sqnl01 081412data-uv xlsx Entered by: J. Sumner Reviewed by:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated E S August 14-21, 2012
- .Statistical Analyses
- EFIonIt frgmetlTl*SolutO n sfle.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVAI SQN 101 End Date: 8/2112012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5100 0.4880 0.5870 0.5530 10.8 0.6730 0.5590 0.6310 0.5940 21.6 0.5990 0.5240 0.5470 0.5820 43.2 0.5920 0.5160 0.5480 0.5880 86.4 0.5090 0.5270 0.5510 0.4480 100 0.5500 0.5650 0.5260 0.4940 Intake 0.6210 0.5730 0.6470 0.4820 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5345 1.0000 0.5345 0.4880 0.5870 8.269 4 0.5744 1.0000 10.8 0.6143 1.1492 0.6143 0.5590 0.6730 7.973 4 -2.807 2.410 0.0685 0.5744 1.0000 21.6 0.5630 1.0533 0.5630 0.5240 0.5990 6.009 4 -1.003 2.410 0.0685 '0.5630 0.9802 43.2 0.5610 1.0496 0.5610 0.5160 0.5920 6.414 4 -0,933 2.410 0.0685 0.5610 0.9767 86.4 0.5088 0.9518 0.5088 0.4480 0.5510 8.649 4 0,906 2.410 0.0685 0.5213 0.9075 100 -0.5338 0.9986 0.5338 0.4940 0.5650 5.806 4 0,026 2.410 0.0685 0.5213 0.9075 Intake 0.5808 1.0865 0.5808 0.4820 0.6470 12.504 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94963 0.884 -0.1675 -1.1562 Bartlett's Test indicates equal variances (p = 0.97) 0.84084 15.0863 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100 >100 1 0.06848 0.12811 0.00527 0.00161 0.02854 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(Exp) Skew IC05 59.875 ICIO >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 0.5 0.4 0 0.4 S0.3 j 0.2 0.1 0.0
-0.1
-0.2
-0.3 0 50 100 150 Dose %
Dose-Response Plot 0.8 0.6.
0.6 1-tail, 0.05 level of significance 00.-
(:30.3 0.2 0.1 01 V 0D 6 File: sqnl 01081412data-uv.xlsx Entered by: J. Su er Reviewed by:
Sage 59 of 99
TVA / Sequoyah Nuclear Plant, Intake UV-treated August 14-21, 2012 0:T Statistical Analyses 1
21 lfmfra Tetn Sttonj.Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/14/2012 Test ID: PpFRCR Sample ID: TVA/ SQN 101 - Intake End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: August 2012 Protocol: FWVCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5100 0.4880 0.5870 0.5530 10.8 0.6730 0.5590 0.6310 0.5940 21.6 0.5990 0.5240 0.5470 0.5820 43.2 0.5920 0.5160 0.5480 0.5880 86.4 0.5090 0.5270 0.5510 0.4480 100 0.5500 0.5650 0.5260 0.4940 Intake 0.6210 0.5730 0.6470 0.4820 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5345 1.0000 0.5345 0.4880 0.5870 8.269 4 10.8 0.6143 1.1492 0.6143 0.5590 0.6730 7.973 4 21.6 0.5630 1.0533 0.5630 0.5240 0.5990 6.009 4 43.2 0.5610 1.0496 0.5610 0.5160 0.5920 6.414 4 86.4 0.5088 0.9518 0.5088 0.4480 0.5510 8.649 4 100 0.5338 0.9986 0.5338 0.4940 0.5650 5.806 4 Intake 0.5808 1.0865 0.5808 0.4820 0.6470 12.504 4 -1.088 1.943 0.0826 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95559 0.749 -0.6317 -0.2536 F-Test indicates equal variances (p = 0.44) 2.69933 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.0826 0,15453 0.00428 0.00361 0.31833 1, 6 Treatments vs O-Control.
Dose-Response Plot 0.8 0.7 0.6 f;
0..
0.4 .. . . . . . ... - - - - - - - I -tail, 0.05level of significance 0.3 0.2 0.1 0
co 0 c; dz File: sqnI01_081412dEtta-uv.xlsx Entered by: J. Sumner Reviewed by age 60 of 99
I*ETS Page 5 of 6 Spec ies: Pmneyhales promelas Date:
Clienit: TVA / Sequoyah Nuclear Plant, Outfall 101. UV-treated Chemnstrv:
Dai IDay I,
m (Analyst identified for each day. performed 0 I pH. D.O. and conductivity 1 I measurements only.)----
2 1
Analyst Concentration [Parameter I DH (S.U.)
I DO (me/L)
Conductivity CONTROL (Ujmhos/cm'l
- Alkalinity UV-treated (mg CaCO3/L)
- Hardness (Mg CaCO3/L)
I .,v ...... . .... m*
________ T emuperatureUL .. .*a IL, 66 11~ wt -1 pH (S.U.) 7I.CC '7'f 7.5a 1S 1C DO (mg/L) __ _ __ 7,_ _1 __ 7.q 10.8% Conductivity vqzBZIS (pimho sLq 7.4 0
_______*Temperature CC) ILAI VA- I 'L-* (
pH (S U.) '7.CC 1-747 7.50 IZ C DO (mgfL) V 1 7.4 7 21.6% Conductivity (jimhos/cm) 7-9Zg4 e
______*Temperature (C) '8 -L4 1. _& 11 pH (S.U.) 7.4S 7.Llq I -i'Ll l7.CftQ~~L DO (mg/L) 43.2% Conductivity (nhsfcmn) Z52
- Temnlerature ('C) a a - -
PH (S.U.)
DO (mp/L) -8. C3'7.Lo 86.4% Conductivity (jpmhocm
- Temperature (QC)
S S~
~u tv ii~ I ?ICZ -q3 6 7..9 PH ru.
DO (ma/L)
I" 0 ~~7.4579 Conductivity (iunhos/em) 100% -Alkalinity (mg CaC0iIL)
- Hardness m
S(me CaMO/L) 1'. 0 -S.
-I
- TR chlorine (mg/L)
I ?_
Co7.6 . .5
- Temperature (OC)
DH (S.U.)
(O.I6 DO (ma/L)
Conductivity (pimhos/m 100% *Alkalinity
______I Intake (mg CaCOs/L)
(mg
- Hrdness" CaCO3lL)
- TR chlorine (mg/L)
- Temperature (Q Ij -(o.
Final Initial0.0 . .
"t.Final IS Initial ... Final.
! I
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table locad onPi Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to ths be
.__ . Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.
- SOP AT20 - Exhibit AT20.3, revisd:n07-1 age 61 of 99
&M-ei
- ,.. Page 6 of 6 Species: Pbnephalespromelas Client: TVA / Seaunah Nuclear Plant. Outfall 101. UV-treated Date: O&lLt.- .
Day (Analyst identified for each day, erformed PK DO. and conductivi measurements only.
3 4 5 Concer- Parameter trati0n I
pH (S.U.) * -,7 1.S, 7.0 7." . 77. 7 Conductivity CONTROL (jirnhoslcm)
UV-treated *Alkalinity (mg CaC03/L
- Hardness (Mg CaCO 3/L) *2. IA-. 2.4- i
_________*Temperature (O*
- PH (s.u.) .8 74 7i.67 - 7.7 C1( .3 10.8%
DO (mnW Conductivity
-7.
li .
I
- TemperatureC)C I n. S.O a.. "M44.1 PH, U.) . -.
- 7. ".* _ o___ .. C .3 DO (mgpL) 0 -7. .. 1 I 21.6% Conductivity (11mhos/cm)2-p pH(S.U.)
(Q) 11.
-_*Temperature o
-7 54 IIs'. I.
s I
DO (mg/L).'8" 7.9 7..I9.0 1.9 7.9...
43.2% Conductivity (gxmbos/cmn) LC-L17
_____*Temperature (OC) L & ~ Is .- .. ~U.0 if'.
PH (S.U.) 7. 7.603 .*I 1 7. 7.3 DO hlorin1 .mA 7.j.
86.4% Conductivity
-Ios/cm 1% *Alkalinity 167 1 (%mg CaCO3/L 0 V *Hardness
- Temperature (IQ) "I-%. 2LS "...
PH (S.U.) i~
7.47LI 7A.2 sq 7.
DO mglL) I 7..0.97. dq
Whos/cm) 176 (Sol 100% *Alkalinity10 Intake (mg CaCOs/
- Hardness (Mg CaCO31L)1-
- TR chlorine (mg&L) 0
____*Temperature *Q IS. 1.(.f 2q. 11.Z S I I .7 s Initial Final Initial 11 Final I Initial 11 Final I Initial I Final
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.
Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bcnch sheet Total residual chlorine was performed on non-treated OutflI 101 and Intake samples.
SOP AT220- Exhibit AT20.3, revision 07-01-12.
\*jP~yage 62 of 99
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated August 14-21, 2012 PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 0
Daily Chemical Analyses cc Environmental Testing Solutions, Inc. Project number: 8207 Concentration Parameter Da 0 Day I Day 2 Da Da y4 Day 5 Day 6
_____________________ Initial Final Initial Final Initial Final Initial Final Initial Final Initial. Final Initial Final PH (SU) 7.65 7.49 7.50 7.59 7.62 7.53 7.59 7.47 7.56 7.40 7.59 7.61 7.62 7.34 DO (rneIL) 7.8 7.8 7.9 7.8 7.9 7.9 8.0 7.8 8.0 7.6 8.1 7.9 7.9 7.8 Cnrl 309 1302 303 299 929 M31610 cnrl Alkalinity Conductivit(mglL(Imhos/cm)
CaCO 3) 302 62
,298
.. 61 61-Hardness (mg1L CaCO3) 84 .j84 RE2784
_______Temperature COC) *24.8 247 24.8 24.7 24.8 24.9 24.9 247 24.9 24.8 24.8 24.6 24.9 2.
PH (SU) 7.66 7.48 7.50 7.56 7.63 7.52 7.62 7.45 7.58 7.41 7.57 7.57 7.61 7.34 DO (mg/L) 7.9 7.8 7.9 7.8 7.9. 7. 8.0 7.8 8.01 !7.6. 7.9 7.8 7.9 7.8 0.% Conductivity (Iwnboslcm) 294 . 298 293__ 291__ 285__ 282__ffV2X_
_______Temperature (90 24.9 24.5 24.8 24.7 24.9 25.0 25.0 24.7 25.0 24.8 24.8 24.7 24.9 24.5 PH (S11) 7.66 7.47 7.50 7.52 7.62 7.52 7.63 7.45 7.56 7.46 7.57 7.58 7.61 7.34 2.% DO (mal) 7.9 7.7 7.9 7.9 7.9 7.8 8.0 7.8 7.9 7.8 8.0 7.8 7.9. 7.9 2.% Conductivity (pmhnos/ci) 281__ 284__ 280_ 277_27_27_28
_______Ternperature CC) 24.9 24.81 24.8 24.7 24.9 -24.8 25.1 24.8. 25.0 24.8 24.9 24.7 25.0 24.6 PH(Sim 7.66 7.45 7.49 7.54 7.62 7.52 7.62 7.45 7.55 7.411 7.54 7.58 7.61 7.33 DO (mgtL) 7.9 7.7 7.9 7.9 8.0 7.9 8.0 7.8 7.9 7.8 8.0 7.8 7.9 7.9 4.% Conductivity (pinhostcm) 255 * ~ 258 .. 257 255__ 246__ 249__ 256__
_______Temperature (*C) 24.9 24.7 24.8 24.7 24.9 24.81 25.1 24.8 25.0 24.6 24.9 24.8 25.0 24.6 PH (SU) 7.63 7.43 7.48 7.56 7.59 7.50 7.61 7.45 7.50 7.38 7.49 7.57 7.59 7.33 DO (nmg/L) 7.9 7.7. 7.9 7.9 8.1 7.9 8.1 7.7 8.0 7.5 7.9 7.8 7.9 7.9 84% Conductivity (ismhos/cm) 204 =07 2JM - 20'7 ~. *'204 197 ___ 98_ -207__
_______Temnperature (0 C) 24.9 24.7 24.9 24.7 24.9 24.8 25.1 26 25.0 24.71 24.9 24.8 25.0 24.3 PH (SI1) 7.62 7.43 7.48 7.54 7.591 7.49 7.61 7.2 7.50 7.41 7.47 7.57.59 7.31F DO (mWL 8.0 7.7 7.8 7.9 8.31 7.9 8.1 788.1 7.5 8.0 788.0 7.9 Conductivity (gmhos/em) 16188, 18'6 182 178 181 .187 [N M 100% Alkalinity (mg/L CaCO 3) 70 .. 70 '70 Hardness (mgfL CaCO3) 72 . . 72 . 72
- Total Residual Chlorine (mg/L) <010 <0.10<01
_______Temperature COC) 25.0 246 25.0 24.6 25.1 24.7 25.1 24.6 25.1 24.7 25.0 24.7 25.1 24.2 PH (SlY) 7.60 7.42 7.48 7.55 7.57 7.53 7.61 7.43 7.47 7.42 7.461 7.58 7.59 7.32 DO (mg%) .8.10 7.7 8.0 7.9 8.2 8.0 8.1 7.8 8.1 7.7 8.0 7.9 7.9 8.0 Conductivit (Iwhos/cm) 186 -IO 191 184 182 & M .YJ 178 178 189 MWMN 100% Intake Alkalinity (mgfL CaCO3 ) 70' 70 .... 7 Hardnesa (mg/L CaCO3) 71 72 7
ýTotai Residuai Chiorine 'es.1w <011W.1 <0. 10 Temp erature (0) 25.1 2. 250 24.61 25.0 24.9 25.1 24.61 24.9 2.48 2.512.
- NOte: .otal residual chlorie was performed on non-treated Outlall 10l and Intake samples.
File: sqnl01_081412chem-UV.xls Entered by: J. Sumner Reviewed by: b
.ETS Page -7 Page I of 3 SEtwimnmentBITesting Solutions, Inc.
Alkalinity (SM 2320 B)
Matrix: Water, RIL =1.0 mg CaCO3 /L Analyst ] Time initiated 15 0, Date analyzed Ei.iK.ZZ Titrate samples to Time completed ict pH = 4.5 S.U.
Thtrant normalitv and multinlier detenninatlos
. . . . no. . .. and T .. . . . .... .. . a p11 of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total f (5 ml Na2 CO 3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E = Nx 500
= 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) 5.1 %0%q~ 494 10119 it 0.0 oNSo ~ o 1o.3 tote: A*- -1k Laboratorycontrolstandard:.
Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO3/L) (acceptable range (Ing CaCO31L) (ml) ml ml ml =90 to 1!0%)
io3t" 100 100 it. I tl.* V,.9 10.3 9q 1.90,%
Duplicatesaf pie precision: ______ _____
Sample Alkalinity %RPI) =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) ((S - D)/[(S+r)g2-) x 100 number (ml) ml ml ml (acceptable range=.+ 10%)
Duplicate (B) D Matrix spike recovery: ....
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Ing CaCO3IL)
(mg CaCO3IL) (mi) ml ml ml
-fN$I tO37 sO I0L> Z7.7 -$3.S 16.g (o.3 Iio Sample alkalinity (B) Measured spike value (MV) %. R = MV / SV x 100 (mg CaCO 3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75 to 125%)
- '-19 ' ¶ :oL.
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Ing CaCOL) 00a *04"AL lSLO H t 30,S 14.S 6.0 103 (OZ
.I..t ___ __ . 0.0 6.o *.o ___ _______
001 GaA4i6L __ G.) 117.3 11.3 1_ ___ _______
"Oe.lmIffl, _* 17.3 LS.3 11.0 1 110
__.___r___, ____ Z1.3 31.t- o.q I U0
_ GO 35.1. 4S.7 C-5 CZ) Go
- MAC,- 'H. I 1 100 0.0 Co G.o0_
Reviewed by: I IJX Date reviewed: 00t (.
Page 64 of 99 SOP C6 -Exhibit C6.1. revision 06-01-11
.ETS Page -7t Page 7. of 3 1 Endonmtnjle In Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst I F . Time initiated Date analyzed Iog.vI.]t Titrate samples to Time completed pH = 4.5 S.U.
Titrantnormalltv and multiplierdetermination:
pH of Normality Normality (N) of H2S0 4 pH Factor or Multiplier DI)O"RI"T- -- Ttrant--_ft iýn End Total = (5 ml Na2CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water reference -standard ml *
- _ = 0f,5/E ffiNx 500
= 4.5 S.U. number number _0220) (E)
Laboratorycontrolstandard:.
Reference standard True value Sample Alkalinity (MV) %RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCOsIL) (acceptable range (119 CaC3/L) (ml) ml ml ml = 90 to 110%)
0 _____
I,, 3- 100 100 7.9 q5 .3 Duplicatesampie precision:
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) {(S - D) /l(S+l)/2]) x 100 number _ _ _ _ _ ml ml ml (acceptable rarge = I 10%)
Duplicate (B) 1,1 3 A D 7Z 72 Matrix spike recovery: -
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(me CaCO 3/L) (mln) mli ml ml V-15S 1037 too J 3o.9 -3q.o s.Z ) 1io.3 17o Sample alkalinity (B) Measured spike value (MV) % R = MV /SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mr CaCO3/L) -75 to 125%)
72 . q.oy.
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml Iml ml Multiplier (me CaCO3L) ao0jt1.tO
&f/A' IS. 0 nu ) 31.o qZ.4 3.4 ftjO1 . 76 I-LoS .o& 4 ,_
LCLAI 45A 3.5 73 I't_.
- ot I-of l I- I. "Tamst~o')1_ krTI 3._
.o0 G4.6 3.3 3.3 77 I____r _.04 C.6. to.0 3.'4 70 I7,01i - 1 -GI AQIiba i0 1___ 10.0 11.4 3.4 70
- z~o~1.O 4/13._ 4. 16.1. 3.4 _____
C~MO V_____ I~ O23.,f %I___ 7b Reviewed by: I[ . 7I Date reviewed: [o et,'l.17-Page 65 of 99 SOP C6 - Exhibit C6. 1. revision 06-01-11
- ETS Page 7Z Page 3 )f _.
En~viistmenitakiting Sltos n Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Analyst I Time initiated Date analyzed [i.1i.1 I Titrate samples to Time completed Z ra pH = 4.5 S.U. 011.112.Z T7trant normallhN,and multiplier determination:
pH of Normality Normality (N) of H 2SO 4 pH Factor or Multiplier
"~m---T-itroat.
reference -- check Begin End Total (5 ml Na2CO 3 x 0.05)/E (Nx 50000)l water ai ý ml ml =
0.25/E
= N x100S0 ml sample
=4.5 S.U. number number. (acceptable range = 0.0180 - 0.0220)
Laborator control standard; Reference standard Truevalue Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) Volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3L) (ml) ml ml ml 90 to 110%)
-X~ss io37 100 10O Z.3.C 3.1 %.5 10.3 '"to Duplicatesam i precision:
Sample Alkalinity [ %RPD=
Sambe S V) volume Begin End Total Multiplier (mgCaCO (mg -D)IRS-D)fl) x 100 Matrix spike recovr:____________
Rfrnce standard Spike value Sample LutiperSpike alkalinity (A) nubr(SV) volume Begin End Total Multiplier (mg CaCO3 /L)
(rag CaCO3/L) (ml) ml ml ml
&sS to17. 103.5 4 .7 42. e-5 \o. 170 Sample alkalinity (B) Measured spike value (MV) %R=MV/SVxl0 (ra1 CaCO3/L) = 75 to 125%)
-70. 100 Sample measurements:. .=
1% Sample volume Begin End Total Alkalinity Sample number ' Sample ID (mi) ml ml ml Multiplier (mg CaCO/L) 1S)'x RO3 _1_6 4.. 4t1 1 3.,4 o 7o
- a. oI f(4Ao) I JoQ o., 1.0 0.9 4 r2L of 1%-a lxatR, \* & _
- I.o q~q 7.1 3.4 3.5 __c 3,'C; as. ....
Reviewed by: [IZ* I Date reviewed: F06t_,9 .
Page 66 of 99 SOP C6 - Exhibit C6. 1. revision 0 6-01-11
AIM SErnvronmentalTesting Solutions,Inc.
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCOA/L Analyst [3 Time initiated& ".
Date analyzed [o.g.i.1 Time completed ".
Tltrant normalitv andmultiblier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA. pH Factor. or Mutiplier reference standard ml ml ml = 0.21E = (N x 50000)/.$1). m'inIipM'0le6 number number (E) (acceptable range = 0.0180 - 0.0220) -Ni 66.0 r:.K 418 Iiss 103 0.0 10o. o. o.ojq iq.c .... __
LaboratorY controlstandard:
Reference standard True value Sample Hardness (MV) % RS=*V/ TV :100 number (TV) volume Begin End Total Multiplier (iag CaCO 3/L) (acceptable range (mag CaCO3/L) (ml) ml ml ml = 90 to 110%)
- )oqS 40 502 l2 .7L!2.7 2- I eli s, Duplicatesample precision:
Sample Hardness %RPI Sample Sample ID volume Begin End Total Multiplier (mg CaCO3lL) {(S - D) /[(S+D)/21) x 100 number (ml) ml ml ml
%Z S 7 7
___ ___ -__ __ 14.- 4-7 14. ____ _---------_N oejLDI~ uplicate (B) .D Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)
(mg CaCO3/L) (ml) ml ml ml 9145's 1041 4o W M~7 z3.7 C-S KC~ 3 Sample hardness (B) Measured spike value (MV) %-R=M/SVxl10 (Iag CaCO 3/L) MV = A-B (acceptable range (Iag CaCO3/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardtiess Sample number Sample ID (ml) ml ml ml Multiplier (ag CaCO3L)
TV = ND Blank (should be = 0 mg CaCO3 /L) D 0.0 0.0 0.. ND
-11_, ' -4 _ z3.7- 7-7. '4 L.5S.I__
)zo~s-I 7.t7.7 .37 '.7 ".4
_______ et.44m PAA ___ 3Zi .4 3.5 1.1 ___
08-m12.i-4 frh%4SIL' 0 _ 33.5 37.c Li.:s 9_
____________3?.S i Z. I 4.3s 64 ___
o
- ____ 4Z.1 '46.'4 L1.3 __.__.
1 14 6.S~.1 1UN-4 h 5W O got_ 0.0 3.7 3.7 ____________
iZO1SIOZ._ _3. 1.3 3. _ __ 71
_______ 1_______
CeI k _____7.~ 1 11 _SI___1-.q_ 741 N*geil6 OlU* is used, sample must be diluted. Reviewed by: I e ] Date reviewed I081+tZI nl........ ..... ......-- .
Page 3q Page IZ of 3 EnvrIoirnfeftltTeatlngSolutIans, Inc.
Total Hardness (SM 2340 C)
L Analyst a Date analyzed[~ 3 ]
RL = 1.0 mg CaCO 3/L Time initiated Time completed
[7 C
-Z._
Titrant normality and mult~ipier determination:
Ireference number Titrant Normality ldll numbercheck Begin al End rE) i-Total (accepta
= 0.2E Normality -(N) of EDTA 0.0220)
-=(N x 50000)/ *50 ml sample
=Nxor11000 pH Factor Multiplier I I. Laboratory controlstandard:
Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mng CaCO3 /L) (acceptable range (Ing CaCO3/L) (ml) ml ml ml = 90 to 110%)
VMS$ 16ki 40 50 11. M 13.1 K.
qC "57 -6.**
Duplicatesample precision:
Sample Hardness %RFD Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L) {(S - D) /[(S+:D)/2]) x 100 number (ml) ml ml ml s-2otio*i.-I TV SWi wT I d) 1C.7t 32.... 119 7Z.
Duplicate(B) -- .'7 D Z-Matrix spike recover: ....
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (iag CaCOa/L)
(mg CaCO3sL) I ml mi ml xj,3,ss 10q S- 4c, 1C.-7 "t-ZA 5.1* 1t.C10 Sample hardness (B) Measured spike value (MV) %R=MV/SVx 10 (mag CaCOd/L) MV = A - B (acceptable range (Ing CaCO3/L) = 75 to 125%)
"/2.9 S.;01.0 Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (ag CaCO 3/L)
TV--N- -- a Cs -11.t, mz.
(should be = 0 mg CaCO 3/L)
. 1-4.. _0_ _ 41.q .3 "5 111111111.-r 7z.
i aLoA.V,01 -TVAS JIOIUU 1 q.q 33.C 3.7 7Z.
I.39tljO 1 2- 3S.C 37.'j . 7%.
_-Z.O__ _O_ -3 _ W. 3 "'f.4 3.7 7Z
_________Z. __ _ '44.c ~413 3.7 1 M v~a~l1.O¶ 30.0 3S.'? 3.7 ___ _______
vuLOSIa4.0 f AUOV aJ1____ 3.1 1A LA' 1___t Ll I is used, sample must be diluted. Reviewed by: " w. Date reviewed q ' I ]
SOP C7 - Exhibit C7. 1. revision 06-01-11
Page o Page 3 of 3
~Envfronme~ii~sigSolutionsj nc.
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO 3/L Analyst Time initiated Date analyzed [InId*q 5iliIIIIIIId Time completed -'I Titant normnality and multipler deltermination:
erIeffft-number Titrant 5
numbercheck Normality
,,i Begin ml End ml (E)
Total (acceptable
= 0.2/E range(N)
Normality
- of EDTA
= (N x 50000)l 5O ml sample
=Nxor1000 pH Factor Multiplier ~1 j
Sample volume Begin End Total Hard ness Sample number Sample ID (ml) ml nml ml Multiplier (mg Ca(O0/L)
(should be = 0 mg CaCOJL)
,1. ,,10.0' F.LLW .& IL. AD -7.7 I1.o 3.3 Iq.C ,_s vt-o*% t.0 J/4 -3' 4iI1.6 15.5 'is ,
P >4jf~l~tiW*t is used, sample must be diluted. Reviewed by: I] Date reviewed n-iII]
SOP C7 - Exhibit C7.1. revision 06-01-11
Page qz Page 1 of_ o
'PETS Inc.
EnvironmentallbongSo~lgOutionls, Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst ;T%3 I(odidereagent: T"Nr 514C Date analyzed Oi. 1 -I. Acid reagent: I tSri I Calibration:
0.10 img/L - 1.00 mg/L I Slope Reference standard number > 1.06 -of the saCples -mg/L, I Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV X 100 number (mg/L) . (mg/L) (acceptable range 90 to 10%)
- rj~ss Io*-t 0.50 o.Szq log.-
Duplicate sample precision: ..
Sample Sample 11D Sample characteristics Residual chlorine %RPD = [(S - D) I[(S+D)/21) x 100 number I(MgL (acceptable range =:i: 10%)
o*'4.E PGLAhjctb 1 d' S10t1I..
4, DuplicateD____ 68 4V -
Sample measurements: _____
sample Sample 10D Sample characteristics Residual chlorine number _ __ .__
Reagent Blank 1.- DOI?
Note: All samples were analyzed inexcess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard Trevau (TV) IMeasured value (MV) %RS MV/TV x 100 number I mg/L) I(mg/L) (acceptable range = 90 to 110%)
12 *.s 1064
\ 0.50 0.471 Reviedr. .yZII, Reviewed by " *\'" ]
Date reviewed SOP C8 -Exhibit C8. 1, revision 06-01-11 Page 70 of 99
Page -tS Page _ of i 6
".ETS Envlmnmental Testing Solutlons6 Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter Accumet Model AR25 pH/Ion Meter Analyst [0 I,odide reagent: F sic Date analyzed 061.lC. Acid reagent: [ Jz L slP Calibration:
- .. 0.10 mg/L I 1.00mg/L ] Slope I rReference standard number -X14SS lOC 1 o 0 -4.ocY 771 Note: For samples with a residual chlorine of > 1.0 mg/L., the samples must be diluted to be within the calibration range.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) %RS=MV/TVx1001 number (mg/L) (mg/L) (acceptable range = 90 to 110%0)
CArSs 10CL 0.50 O.53% IoG.q]
Duplicatesampleprecision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]) x 100" number (mg/L) (acceptable range = k10%)
1 S 1,70916 5 03"'llIftt (-6.0017-Duplicate I -. oC1 S Sample measurements: .....
Sample Sample ID Sample characteristics Residual chlorine number _ _ _ _ _ _
Reagent Blank r 0.00?6
- v2A&tlS..
k c..6SQWti.
toeLsg..__.___---_ no\CcAr,clear lZ 08 C...1~A- CO).(~
co (4 r c(er, pqr4Les L.O.00 t53 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) % RS =MV /TV x 100 1 number (nmg/L) (11g/L) (acceptable range = 90 to 110%) I Z.4 1 C.1 0.50 o. cs q*.o%
Reviewed by Date reviewed Page 71 of 99 SOP C8 - Exhibit C8. 1, revision 06-01-11
Page 7 Page _of I "ETS Env*ronmentalTestlng Solutions, Inc, Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst a's [I Date analyzed o*e.1.lZ Calibration:
[ I odide reagent:
Acid reagent:- [-*ItI-t s7i7c
-0.10 mg/L 1 1.00 mR/L 7 Slope Reference standard number I Irtss 10C4 r.qac.
1
- Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within' the calibration range.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) % RS = MV/TV x 100l number (mg/L) (mg/L) (acceptable range = 90 to 110% J Djss 1o0q 0.50 o.SZo i0'.o% J Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]) x 100 number (acceptable range = h10%)
Duplicate D 0
'.001 ----
- co .tM Sample measurements: .....
Sample Sample ID Sample characteristics Residual chlorine number ,__m__/L_
Reagent Blank "0o.oo07 1 M000____ -5 A~ aaTAAbcolor, fl0 clce'r _____
12,086~ 18 0 h wo% lh . Poils: -Se1au, Jee.r-f Lo.06 4Lf j9J%96q 6 ¶'IqA 1 SQ~va tot.003fy
_________ 'L, flcotorcd,,efe.
rtAce jPPAJ&S L~c3 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory controlstandard:
Reference standard True value (TV) IMeasured value (MV) %RS=MVITVx 100 number I(mgIL) I(Mg/L) (acceptable range = 90 to 110%)
i 16C4 0.50 Oaiqcit.I I Reviewed by Date reviewed LJA0-LLJ SOP CS - Exhibit C8. 1, revision 06-01-11 1Page 72 of 99
Sequoyah Nuclear Plant Biomonitoring August 14 - 21, 2012 Appendix D Reference Toxicant Test and Control Chart Page 73 of 99
E Pimephalespromelas Chronic Reference Toxicant Control Chart SEnvironmental Testing Solutions, Inc. Organism Source: Aquatox, Inc.
I 1.0 I I I I I I I I ~ I I I I 1 7--
USEPA Control Limits (+ 2 Standard Deviations) 0.9 F-0.8 .- ...... ........................... k....................................'
0.7 0.6 0.5 [-
I II I I I I I I I I I I I I I I I I I 1.4 USEPA Warning and Control Limits (75 and 90 Percentile CVs) 1.2
.J 1.0 S0.8
>1 0.6
'ý 0.4 I I I I I I I I I I I I I I I I I I I I 1.4 I I I I I I I I I I I I . I I I I I I I I Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1.2 1.0 0.8 ........................ ......
0.6 0.4 I I I I I I I I . I I I I I I I I I I I Test date
-.------ 7-day IC., = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 +/- SA.10 or 5A75)
Graphs generated from associated excel spreadsheet.
Excel spreadsheet entered by: J. Sumner Control Limits (mean IC25 +/- 5 A.25, SA.90' or 2 Standard Deviations) Reviewed b. k
ýý age 74 of 99
Pimephalespromelas Chronic Reference Toxicant Control Chart 0
- Environmental Testing Solutions, Inc.
State and USEPA Laboratory Laboratory USEPA USEPA Test number . Test date 7-day IC cT T Control Umits Sw Warning ULmits S3.s Control ULmits SK"S Warning Umits S,.. Control Umits CV L Kga) (g/L Kd) CT-2S CT+2S CT-SAs CTa+SA1 CT - S. CT+ SA.. CT - S*. CT + S&s CT-S&9 CT+ Sa.g 1 08-09-11 0.67 0.71 0.05 0.62 0.80 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 2 09-13-11 0.66 0.71 0.05 0.61 0.80 0.08 0.62 0.79 0.15 0.56. 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 3 10-04-11 0.73 0.71 0.05 0.61 0.81 0.09 0.62 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 4 10-11-11 0.76 0.71 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.03 0.07 5 10-18-11 0.67 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 6 11-08-11 0.78 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 7 12-08-11 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 8 02-07-12 0.77 0.05 0.61 0.81 0.09 0.63 0.80 0.71 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 9 02-14-12 o.74 0.05 0.61 0.82 0.09 0.71 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.07 10 03-06-12 0.64 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 11 03-06-12 0.68 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 12 04-12-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 13 05-08-12 0.77 0.72 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.08 14 05-15-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.08 15 06-05-12 0.72 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 16 06-12-12 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 17 07-10-12 0.76 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 18 07-17-12 0.73 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.07 19 08-07-12 0.70 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0AS 0.99 0.32 0.40 1.04 0.07 20 08-14-12 0.81 0.73 0.05 0.63 0.82 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.06 Note: 7-d IC. = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean IC2s).
S = Standard deviation of the IC25values.
Laboratory Control and Warning Umits Laboratory control and warning limits were established using the standard deviation of the lCm3values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
S.sA = Standard deviation corresponding to the 10"' percentile CV. (SAM= 0.12) 5 a.us= Standard deviation corresponding to the 2 P 5 percentile CV. (S&2= 0.21)
USEPA Control and Waming Umlts SA.7 = Standard deviation corresponding to the 7 5 t percentile CV.(S&., = 0.38)
Sa~ss= Standard deviation corresponding to the 9 0 " percentile CV. (SAga= 0.45)
CV= Coefficient of variation of the IC2svalues.
USEPA.2000. Understanding and Aceunting for Method Variability in Whole Effluent Toicty Applications Under the NationalPollutant 0Dscharge Elimination Program. EPA-833-8-09-003.
USEnvlronmentalProtection Agency, Cininnati, OH.
File: ppktci_081412Jdsxt Entered by:. J. Sumner Reviewed by.
99-
0 Pimephalespromelas 0
Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements; Environmental Testing Solutions, Inc.
Organism Source: Aquatox, Inc.
I ".
I I I I I I I I C I ( 0I mI Is I I I
-- 1.00 l2
- 0.75
- -0.50 0 t0 E0.25
- USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
- I I I I I I I I I I I I I I I I I I I -
.- I I I I I I I I I I I I I I I I I I F --
30 C Kentucky Acceptance Limit (< 30.0%)
' a. 20 :...
20 --.
W . a-30 10 USEPA Upper PMSD Bound (90'h percentile < 30.0%)
012 0.. .
10 I I I I I I I I I I I III I I
.................... Test.d.......ate . . I ... I I I_
Test date
- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- - Central Tendency (mean Control Growth, CV, or PMSD)
......... Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
Graphs generated from assodate1 excel spreadsheet.
Excelspreadsheet entered by: J :umner
ýýage 76 of 99 Reviewed by:
Precision of Endpoint Measurements SPimephales promelas EnvironmentalTestinggSolutions,.In. Chronic Reference Toxicant Data Test number Test date Control Control Mean cv Cr MSD PMSD CT Survival Growth for Control Growth for Control W for PMSD K (mg/larvae) (mg/larvae) N Growth CV (%)
1 08-09-11 100 0.662 6.6 0.07 10.6 2 09-13-11 100 0.848 0.755 6.7 6.7 0.12 13.6 12.1 3 10-04-11 100 0.768 0.759 16.8 10.0 0.13 16.9 13.7 4 10-11-11 i00 0.681 0.740 4.3 8.6 0.07 9.9 12.8 5 10-18-11 100 0.693 0.730 4.8 7.9 0.07 10.0 12.2 6 11-08-11 100 0.792 0.741 10.3 8.3 0.12 15.6 12.8 7 12-06-11 100 0.738 0.740 15.7 9.3 0.12 15.9 13.2 8 02-07-12 100 0.662 0.730 10.5 9.5 0.09 13.4 13.2 9 02-14-12 97.5 0.658 0.722 5.2 9.0 0.07 10.1 1.2.9 10 03-06-12 100 0.854 0.735 3.5 8.4 0.09 10.8 12.7 11 03-06-12 100 0.873 0.748 5.1 8.1 0.10 11.4 12.6 12 04-12-12 97.5 0.706 0.744 7.0 8.0 0.06 8.7 12.2 13 05-08-12 100 0.613 0.734 8.1 8.1 0.08 13.1 12.3 14 05-15-12 100 0.697 0.732 5.9 7.9 0.07 9.5 12.1 15 06-05-12 97.5 0.596 0.723 3.8 7.6 0.06 9.4 11.9 16 06-12-12 100 0.723 0.723 6.0 7.5 0.11 15.6 12.2 17 07-10-12 100 0.641 0.718 5.2 7.4 0.10 14.9 12.3 18 07-17-12 100 0.638 0.713 10.9 7.6 0.07 11.4 12.3 19 08-07-12 97.5 0.660 0.711 6.7 7.5 0.06 8.7 12.1 20 08-14-12 100 0.533 0.702 5.4 7.4 0.07 13.1 12.1 Note: CV = Coefficient of variation for control growth.
Lower CV bound determined by USEPA (10d' percentile) = 3.5%.
Upper CV bound determined by USEPA (90 td percentile) = 20%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD Isthe minimum percent difference between the control and treatment that can be declared statistically significant Ina whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10 th percentile) = 12%.
Upper PMSD bound determined by USEPA (91P 0 percentile) =30%.
Cr = Central Tendancy (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-O03. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-8-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: ppkclcr_0814]2.xlsx Entered by: J. Sumner age 77 of 99 Reviewed by: __
- ETS Page I of 5 Env~roarnentalTn~tngSouinIs Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number: _1.
Dilution preparationinformation: Comments:
KC1 Stock INSS number: 114sý _ _o__
Stock preparation: 50 g KCI/L:
Dissolve 50 g KCI in 1-L MilHi-Q water.
Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organisminformation: Test information: _
Organism age: __".oAX_____ 0._3t) Randomizing template:
Date and times organisms oa- b. r oiv. o Incubator number and were born between: shelf location: _ _ _
Organism source: t * , " Artemia CHM number: CJltt L.
Drying informationfor weight determination:
Transfer vessel pH = "1 eO* S.U. Date / Time in oven: e6&t- -a- i't 0 It information: Temperature = 0,A C Initial oven temperature:
Average transfer volume: Date / Time out of oven: fL.fl. la
- 0. 'L "A Final oven temperature: 1 ,
Total drying time: mg-A*
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 0 __-_L %So*0. VL.S 090 .0-
,-IS.. I.
02 IMlt0(3 1*t % A( It'~
ms .) *t- IL.
4 at-I fl. I?.. 01A1 0116 Mh~.
5__ 08 -Jr.r lgQ- I- 61c AA~ 4 j... loPat-6 0110-mi.. O100 Vs oa 117. r% at- 16 I-).
-724 iot, -21 Controlinformation: Acceptance criteria Summary of test endpoints:
% Mortality: .. IT. < 20% .7-day LCs &U. 'L(,
Average weight per initial larvae:. O.-31 N NOEC 00 Average weight per surviving larvae: (. 12 !0.25 mg/larvae LOEC ":! 0 ChV (016.no IC25 I d1S SOP AT21 -Exhibit AT21.1, revision 06-01-11 (Iage 78 of 99
Page 2 of 5
.ETS (b Envtmnn-r0ITestlng Solutlom In Species: Pimephalespromelas PpKC1CR Test Number: W)$_
Survival and Growth Data Day Control 450 mg KC[/L 600 m KCI/L
-.A B C D. E F G H I J K L 0 101 0 1to It tO Oto 0 to /(I O /1 /0 1 10 10 to 1 10to 10 t 10 1.0 £ /00) 2 10 tO tO 1O to /O/o/Oto to /0 (O 3 (o t( (t 0 10 to t to 10 10 10 10 (0 4__ _1 10 10 10 10 l. tO 0 qI& 1'A (0 /0 5 /0 /0 /0 t0 t0 /0 /0 /0 ' C)
O 0 6 / i "0 U 10 I1 I )/0 1to(0. IO 7_0 tO_/0'O 10 1O0 to 'o'i 10 to /0 A = Pan weight (mg)
Tray color code:: 91;t, Analyst:. J* 130*.* 4 i.q 1 48 '. 1 138 3.3 143 14.4 l t12.1f.91 I a.&Ij I3 ; -&.4-2 Date: 0R.o1.t_
B = Pan + Larvae weight (mg)
Analyst: 40 1017 19 53 7A46 Iq.-3q 14.66 I'l. Zo.ll V-50 19.50 .o.31 14.40 I9.1Z Date: o 194.7.. "
- C = Larvae weight (rag) = B - A Hand calculated. S AA* ,4 S5. s. $ie .1 I. ,.E1 q.L 6i.'* S.S (j.16 Analyst:
Weight per Initial number of larvae (mg)
= C /Initial number of larvae A, A, 0 Hand calculated. 'V '4 IV Analyst:
1/2 '4 C, 1/2 0* 0* 0 0 Average weight per initial Percent -. L.7 0 - - , L number of larvae (mag) reduction.
MI_
from control 0.%
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.
Comments.
SOP AT21 - Exhibit AT21. 1, revision 06-0 1-11 I age 79 of 99
Page 3 of 5 SS*ETS Species: Pimephalespromelas PpKCICR Test Number-. -IS Survival and Growth Data Day 750 mi KCIL 900 mg KCI/L 1050 mg KCI/L M N 0 P Q R S T U V NV X 0 /D t0o 0 10 10 to I0 /0 /0 /0 /o /0 20 M :~ to 0 1- ; -Qa.~'
4_b t'_ "_ " i,- _"z 2_
3 1C0 0% Ilk l_.
__ __ 6 __ &" cj** - u -i 5- I',x .
A = Pan weight (mg)
Tray color code::
Analyst: [4B17. 3.18 1zS 1 13s. 4.-.s*.13.42 IZ.
U 14. it1.05 13IS. 13.O& 13.1f Date: __ _ __ _ _ ._ _ _
B = Pan + Larvae weight (mg)
Analyst: ,,.gj OfE't,.z q.'a.. l rTBqf 17. ZS I i7.1O " tq.7* -
Date:
C = Larvae weight (mg) = B - A Hand calculated. qLii4),s S.11. 3.1t. S,'S t*,'s aoA O.*S IlS&
V Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae
_ .O 4. 0 Hand calculated.
Analyst: ... A I' *' "
Average weight per Initial Percent number of larvae (mg) reduction .O.U - io.
from control Comment codes: c = clear, d = dead, .fg = fungus, k = killed, m = missing, sk = sick, sm - unusually small, 1g = unusually large, d&r = decanted and returned, w = wounded.
Comments:
SOP AT'21 - Exhibit AT21.1, revision 06-01-11 ge 80 of 99
(D Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 0
Quality Control Verification of Data Entry, Calculations, and Statistical Analyses
"* Environmental Testing Solutions, Inc. Test numben 255 Test dates: August 14-21, 2012 cowetrbniIneM/LXM Itp!na hnumbrnrerof MW Reinrmehnof A.P-Wft (nfq 0 Pes-tan Lan-e. rnia5j: Wdlit/Sul*veitn MAffi-W61ht/Sunung. Ca.errM ofnmtirton iuh1tlInffm.nunrbnrof M..rurcta(ll )MuNJ ihti~klfth Co.:Mfetnfvnitticn Per-tredunaonofm I- banta nelet ns A-0 '-abWenfhme. lea) urenrhmolme. ee mtv ane.lief number ofiur. ) mnb)l (%j
-.- 4 m) (%) (-1d A 10 10 13.63 19.27 5.64 0.564 0.564 Control B 10 10 13.46 18.53 5.07 0.507 0.533 5A 0.507 100.0 0.533 5A Not applicable C 10 10 14.89 20.40 5.51 0.551 0.551 D 10 10 14.13 19.24 5.11 0.511 0.511 E 10 10 13.88 19.66 5.78 . 0.578 0.578 450 F 10 10 13.43 19.19 5.76 0.576 0.607 5.9 0.576 100.0 0.607 5.9 -13.9 G 10 10 14.54 20.81 6.27 0.627 0.627 H 10 10 15.02 21.50 6.48 0.648 0.648
.1 10 9 13.81 18.50 4.69 0.521 0.469 600 J 10 - 9 14.69 20.32 5.63 0.626 0.586 8.2 0.563 95.0 0.558 11A K 10 10 13.62 19.40 5.78 0.578 0.578 94.5 L 10 10 1342 19.62 6.20 0.620 0.620 M 10 8 13.12 17.89 4.77 0.596 0.477 750 N 10 9 14.56 19.24 4.68 0.520 0.547 7. 0.468 87.5 0.478 5.2 10.5 O 10 9 13.78 18.31 4.53 0.503 0.453 P 10 9 12.86 17.98 5.12 03569 0.512 Q 10 5 14.59 17.69 3.10 0.620 0310 900 R 10 6 13.72 17.28 3.56 0-593 0.607 4.4 0.356 57.5 0.348 12.4 34.8 5 10 7 12.18 16.23 4.05 0.579 0.405 T 10 5 14.11 17.30 3.19 0.638 0.319 U 10 1 12.85 13.36 0.51 0.510 0.051 1oso V 10 2 13.13 14.76 1.58 0.790 0.650 30.5 0.158 7.5 0.052 142.6 90.2 W 10 0 0.00 0.00 0.00 0.000 0.000 X 10 0 0.00 0.00. 0.00 0.000 0.000 Dunnett's MSD value: 0.0699 MSD = Minimum Significant Difference PMSD: 13.1 PMSD : Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxcity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboretory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent ToxicityTest Methods, Volumes land 2-Appendix. EPA-821-1-01-004 and EPA-821-8-01-005. US Environmental Protection Agency, Cincinnati, OH.
Filme ppkcicr -_081412.xlsx Entered by:
Reviewed b. J._4Sumner
- ET Statistical Analyses Larval Fish 3rowth and Survival Test-7 Day Survival Start Date: 8/14/2012 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 8/2112012 Lab ID: ETS-Enviir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-l EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:
Conc-mglL 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 800 0.9000 0.9000 1.0000 1.0000 750 0.8000 0.9000 0.9000 0.9000 900 0.5000 0.8000 0.7000 0.5000 1050 0.1000 0.2000 0.0000 0.0000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9500 0.9500 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 40
- 750 0.8750 0.8750 1.2138 1.1071 1.2490 5.846 4 10.00 10.00 5 40
'900 0.5750 0.5750 0.8820 0.7854 0.9912 11.405 4 10.00 10.00 17 40
- 1050 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4 10.00 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93302 0.884 0.39148 0.28113 Equality of variance cannot be confirmed Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Trimmed Spearman-Karber Trim Level EC50 95% CL 0.0%
5.0%
10.0% 902.19 867.55 938.21 1.0 20.0% 909.49 869.16 951.70 Auto-7.5% 898.26 863.27 934.67 0.9 0.8 0.7 0.6 0 0.5 0.4 0.3-0.2 0.1, 0.0, 1 10 100 1000 10000 Dose mgIL Dose-Response Plot 11 0.9 0.8 0.7 0.6 030.5 S0,4 0.3 0.2 0.1 File: ppkclcr.0814l:t.xlsx Entered by:. . Sumner Reviewed by: ' .
- age 82 of 99
- ETS 0 nl~~?a ~~g.oulkI(
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 8/1412012 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 8/21/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potasslum chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:
Conc-mglL 1 2 3 4 D-Control 0.5640 0.5070 0.5510 0.5110 450 0.5780 0.5760 0.6270 0.6480 600 0.4690 0.5630 0.5780 0.6200 750 0.4770 0.4580 0.4530 0.5120 900 0.3100 0.3560 0.4050 0.3190 1050 0.0510 0.1580 0.0000 0.0000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5333 1.0000 0.5333 0.5070 0.5640 5.353 4 0.5703 1.0000 450 0.6073 1.1388 0.6073 0.5760 0.6480 5.924 4 -2.307 2.180 0.0699 0.5703 1.0000 600 0.5575 1.0455 0.5575 0.4890 0.6200 11.433 4 -0.756 2.180 0.0699 0.5575 0.9776 750 0.4775 0.8955 0.4775 0.4530 0.5120 5.244 4 0.4775 0.8374 900 0.3475 0.6517 0.3475 0.3100 0.4050 12.430 4 0.3475 0.6094 1050 0.0523 0.0980 0.0523 0.0000 0.1580 142.558 4 0.0523 0.0916 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test Indicates normal distribution (p > 0.01) 0.94803 0.805 -0.6553 0.58729 Bartlett's Test Indicates equal variances (p = 0.40) 1.85289 9.21035 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F.Prob df Dunnett's Test 600 >S00 0.06992 0.13112 0.00569 0.00206 0.11571 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL(Exp) Skew IC05 629.55 44.05 422.75 677.99 -1.2511 IC10 683.02 39.17 471.16 745.11 -1.6458 IC15 736.48 32.27 546.98 788.00 -2.5024 1.0 IC20 774.58 14.39 725.29 814.40 0.0156 08 IC25 807.48 14.81 783.34 852.44 0.1502 IC00 902.72 14.24 850.75 928.37 -0.5101 0.8 IC50 931 6i9 908R 905.24 9-5694 01-578 0.7-0.6 0.5 CO0.4 I0.3 0.2~
500 1000 1500 Dose mg/L Dose-Response Plot 1-tall, 0.05 level of significance 1.04 0.3 0.2-Vile:pplciattl1l2.xIs Enteredby: Jl.Suniner Remiwed byr.-
(V age 83 of99
Page 4 of 5 00ITSý Envlmonment.Tesfn Sltl o I Species: Pimephalespromelas PpKC1CR Test Number: _IS Daily Chemistry:
- Day Aaddtified for each da erformed D.O. and conductivity measurements onlyv.
Concentration Parameter
. pH (S.U.5) 77.* Ss.*. *_
D O (mg/L)". 7 . .. . ;" . .. . . " . . ... ' " " . .-
Conductivity 0 ""-* .... ".. : . o CONTROL *Alkalinity (mg CaCO 3/L) O-
"*Hardness .
(mg CaCO 3IL) i '1
,____*Temperature (OCD. A. VA. . it pH (S.U.) .7.73". .'
DO (mg/L) 7.7 . 77
- 450 mg KCI/L Conductivity
- Temperature (IC) "k.4. - . i. S pH (S.U.) 1.*S i.s7 7-4SZ.
l.o 7oI DO (mg/L) 14 7. 7.8 600 mg KCIIL Conductivity
_______ mhos/cm) (C 133 %360 bV N
- Temperature (0QD - "4. . C 7. -3 "__._ -
DO (mg/L) "7.7 "7.0 1 750 mg KCIIL Conductivity (gmhos/cm) 100 1CAO
_________ (op 14. 46
- Temperature T41 pH (S.U.) a .5?-4 .C DO (mg/L) 7.9 900 Ing KCIIL LConductivity
(~mhoslcm) Is1&
_ __ _ Temperature (0) l'4q -Lq.14. 2. ii . .. .ýq pH (S.U.) '711 II7c:; 1 -7.-q I ~113 1 : -18 11 14.-- 1 DO (mg/L) 1050 mg KCI/L Conductivity (jLmh sc) -T Z O70
- Temperature (IC)
STOCK Conductivity -'qo0
_ (Rmho Initial II Final I Initial II Final I Initial 11 Final I\
Ix
- Temperatures perfobmed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page Alkalinity and hardness performed by the analyst. identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
SOP AT21 -Exhibit AT21.1, revision 06-01-11
- age 84 of 99
Page 5 of 5 OET E~wlmmeul'uTesung
&olIw~lo.In Species: Pimephalespromelas PpKC1CR Test Number: 21K Day (Analyst identified for each day, performed pH, D.O. and conductivity measuriements only.)
3 . 4 5 :
_____Analyst am zru3 S 0 :sf V Concentration Paramter pH (S.U.)
DO(mg/L)
Conductivity
... 7..
7.CO i 7.,7 V...
i.Ci .. ., " ".I -
(jinhoscm)31 CONTROL *Alkalinity (nig CaCO3/L) 12
- Hardness
- Temperature ing CaCO 3/L) pH (S.U.) .7.7.I 7.4 - ' 71o 7,L. . 7.l .. .S DO (mg/L) 450 mng KCI/L Conductivity (l'imhos/cm) O)
- Temperature pH(S.U.) O3.-+ -7.S7 7.70 7..77
-. itS 7.7.
-i. 1 DO (mgiL) 7.
50 nmg KCIL Conductivity
- Temperature
.....'" ". U .. .
-(gmhos/m) Q y. 1 7.77
-3 1i I
.73:77 .
pH (S.U.) -. _._7' DO (mg/L) 7.__ .7.'7 900 mg KCI/L Conductivity hTempe r .,6
- Temperature
______ i*.o -iM. 2.M.1 *L*.I. 0 -Ll.6. -A,. i,.44 *-uA.S pH (S.U.) 7.C-' -i.'iq
-a, 7 *Lj 7.1 -7.- 7.7q 7.$
goo0mg KCIIL Conductivity41tO DO (mg/L) -7 17.3 7,C 7. 7.
(Oc) 0M -6 4
- Temperaturei"! S A.. L.l. ',' "1.
Initial I Final. I InitialU I Final I- - Initial 11 Final U
I Initial I Final 0, i I
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located qa Page 1.
Alkalinity and hardfiess performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: 7 V SOP AT21 - Exhibit AT21.1, revision 06-01-11 (f age 85 of 99
Ec Ceriodaphniadubia Chronic Reference Toxicant Control Chart
- Environmental Testing Solutions, Inc.
I I I I I I I I I I I I I l I I I I I 1.14 USEPA ControlLimits (k 2 StandardDeviations) 1.12 1.10 -
1.08 1.06 1.04 1.02 I I t II I I I I I I I I I I I I I I I 2.5 2.0 z
J: 1.5 1.0 N,
0.5 1.4 I I I I I I I I I I I I I I I I I I I I
" Laboratory Warning and ControlLimits (10th and 2 5 th PercentileCVs) 1.3 1.2 H-1.1 1.0 -
0.9
...... .... 0 .... 7-I II I fI~ Ifi I I~L I I 0.8 INN NN NN NN NN -IN NN INN N'111NN N$ N$ 0 N$ -'s N, Ns -o WNs Ok"', NO-N" OSA Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC 2 s)
Warning Limits (mean IC 2 5 1 SA1 0 or SA.75)
Control Limits (mean IC25 + SA 2 5, SA90' or 2 Standard Deviations)
Graphs generatedfromassociatedexcelspreadshleet.
Excelspreadsheet enteredby:J.Sumner
-,. age 86 of 99 Reviewedby fY
.ETS Ceriodaphniadubia Chronic Reference Toxicant Control Chart 8 Environmental Testing Solutions, Inc.
State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7<day IC 2, CT S Control Limits SAM Warning Umits SA25 Control Limits SAL3 Warning Limits S,.O Control Limits CV (g/L NaCI) (WJL NaCI) CT-2S CT+2S CT - S&. CT
- SX0 CT- SAý CT + Sz CT- SA-s CT+ SA.7 CT - S,, CT+Sjue 1 04-05-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 2 05-03-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 3 06-07-11 1.05 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 OA1 1.73 0.01 4 07-12-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 08-09-11 1.06 L07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 09-13-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 7 10-04-11 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 8 10-11-11 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 9 11-08-11 1.05 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 10 12-06-11 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 11 01-10-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 O.59 1.54 0.66 0.40 1.73 0.01 12 02-07-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 O.48 0.59 1.55 0.66 0.40 1.73 0.01 13 03-13-12 LOS 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 14 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 15 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 16 05-08-12 1.06 1.06 0.01 1.05 1.06 0.09 0-98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 17 05-08-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 18 06-05-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0A8 0.58 1.54 0.66 0.40 1.72 0.01 19 07-10-12 1.07 1.06 0.0.1 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0A8 0.59 1.54 0.66 0.40 1.72 0.01 20 08-07-12 1.08 1.06 0.01 105 1.08 0.09 0.98 215 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 Note: 7-d c1,= 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnlo reproduction for the test population.
CT = Central tendency (mean IC-s).
S = Standard deviation of the ICn values.
Laboratory Control and Warning Umits Laboratory control and warning limits were established using the standard deviation of the ICEvalues corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint Sý. = Standard deviation corresponding to the 10e percentile CV. (SAe. = 0.08)
$*j, = Standard deviation corresponding to the 2P percentile CV. (SA25= 0.17)
IJSEPA Control and Warning Umits SAJ.= Standard deviation corresponding to the 75h percentile CV. (S&75= 0.45)
SeA"= Standard deviation corresponding to the 9 0e percentile CV. (S, = 0.62)
CV = Coefficient of variation of the IC, values.
USEPA. 2000, Understanding and Accounting for Method Varlability in Whole Effluent ToAxcity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
File: CdNca0CCRO8Ol72icdsx Entered by: J. Sumnjr Reviwemd by-.:
Ceriodaphniadubia ET: Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Environmental Testing Solutions, Inc.
40 I I I I I I I J I I I I I I I I I i I I 0
0 35 30 0
I.' *U 25 M
0 20 0
0 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
I I I I I I I I I I I I I I
- o40 North Carolina Acceptance Limit (< 40.0%)
.0 O 30 Kentucky Acceptance Limit (< 30.0%)
o-20
~10
- i!! ...................- .......................................... ........-
50 . .I I I I I I I I I I I USEPA Upper PMSD Bound (90'h percentile < 47.0%)
40 30 20 10 S................... .......... ........ I I 11 V
SN NN -N'* 1'ý- NAN N-N"' %A-" 0'* OINI O'vs - sqýv - Ns 'N' ,'SAI, 'bx'ý ,I'N I.
o1b bg-" "b"i "'b" ANý% NTI 'bT lo, lo, 'o-1 Q'S" Test date
- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- -- Central Tendency (mean Control Reproduction, CV, or PMSD)
......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
I . 4-
ý f-nlet . Graphs generated from associate I excel spreadsheot Excelspreadsheet entered by: . .;umner
ý age 88 of 99 Reviewed by: -
" lPrecision of Endpoint Measurements
.E T S 1Ceriodaphnia dubia
,: IChronic Reference Toxicant Data Environmental Testing Solutions, Inc.
Test Test date Control Control Mean CT CV CF MSD PMSD (.Ti number Survival Reproduction for Control Mean for Control (N) (offspring/female) Reproduction (K) Reproduction (%) for PrVlSD (%)
(offspring/female) CV (%)
1 04-05-11 100 33.0 5.9 2.2 6.7 2 05-03-11 100 30.9 32.0 4.5 5.2 1.9 6.1 6,4 3 06-07-11 100 28.9 30.9 6.7 5.7 2.5 8.7 7.2 4 07-12-11 100 32.2 31.3 6.0 5.8 2.0 6.2 6.9 5 08-09-11 100 28.3 30.7 5.0 5.6 1.9 6.8 6.9
.6 09-13-11 100 32.1 30.9 8.2 6.1 1.9 5.8 6.7 7 10-04-11 100 32.8 31.2 6.1 6.1 2.1 6.5 6.7 8 10-11-11 100 31.3 31.2 3.0 5.7 2.1 6.6 6.7 9 11-08-11 100 29.0 30.9 7.6 5.9 1.8 6.1 6.6 10 12-06-11 100 29.3 30.8 5.8 5.9 1.9 6.5 6.6 11 01-10-12 100 32.4 30.9 5.1 5.8 2.4 7.4 6.7 12 02-07-12 100 31.7 31.0 4.2 5.7 2.1 6.5 6.7 13 03-13-12 100 31.1 31.0 7.2 5.8 1.9 6.1 6.65 14 04-10-12 100 29.4 30.9 6.8 5.9 2.4 8.0 6.7 15 04-10-12 100 30.3 30.8 7.1 6.0 2.7 8.8 6.13 16 05-08-12 100 31.7 30.9 6.1 6.0 1.9 6.1 6.13 17 05-08-12 100 32.4 31.0 5.1 5.9 2.0 6.1 6.3 18 06-05-12 100 32.1 31.1 4.0 5.8 2.3 7.1 6.8 19 07-10-12 100 31.2 31.1 4.7 5.8 2.0 6.3 6.18 20 08-07-12 100 29.2 31.0 3.5 5.6 1.9 6.6 6.7 Note: CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (10' percentile) = 8.9%.
Upper CV bound determined by USEPA (90'h percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90th percentile) = 47%.
Cr = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharlge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICRO080712.xlsx Entered by: J. Smnner (t age 89 of 99 Reviewed by: _*-
Page I of 6 0ETS i
/'
Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia CdNaCICR #: I.*
Dilutionpreparationin ormation: Comments:
NaCI Stock INSS number. ltyA. __n_
Stock preparation: 100 g NaCI/L:
Dissolve 50 g NaCI in 500 mL Milli-Q water.
Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organismsource information: Test information:
Organism age: < 24-hours old Randomizing template color .,*
Date and times organisms were bom mGL.01t U. 01% M toW Incubator number and shelf between: location:
Culture board: -
Replicate number:
Culture board cup number-
- 2 3 )4 I 6% 15
'I 110 16 1
17 oi a 9 10 1-YWT batch:
010_________
Transfer vessel infbrmation: pH= i".61S.U1 Temperature = 2q.t*C Selenastrum batch:
Average transfer volume (mL): 0.0 51 -A Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 ot.-.(t. tOi- oI -OsrL _
2 0.f_____-a
.___5 oiq1S 04-0-h11-~ 4 ..
- 6. .O
- . 1"I. CA*.\ , t 0 Control information: Acceptance criteria Summary of test endpoints:
% of Male Adults: 0 .20% 7-day LC50 51400
% Adults having 3 rd Broods: INA 80% NOEC 000
% Mortality: . f7. :520% LOEC I* t3 Mean OffspringFemale: 21 . > 15.0 offspring/female ChV 101K.
%CV: ___ _,S7.1 <40.0 % IC 25 161 SOP AT14 - Exhibit AT14.1, revision 06-01-11 (f age 90 of 99
Page 2 of 6 S6ETS Species: Ceriodaphniadubia CdNaCICR #: Vbt CONTROL / Survival and ReproductionData
- I .... Replicate number Day 111n 2 3 4 5 6 7 8 9 10 21 Young Adult mortality produced I....bi..l
%. I I . 1lt-o I L o - 1.~. L- i 600 -
Youngprodueed n in II n 0-3 Young produced Adult mortalitg mortality [__
a *.*q *.-'%-I L- ~II~ t-- I %-%'I --I FL'-. I--
II I Adult mortality Tot5l young produced "T..."1 Final Adult Mortality X for roodsung __ ,
CT C
f ' i._.._. *C_
Note: Adult mortality (L= live, D ff dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). Cnetao: OT Toa Iyoung produced 3 1w __IL1 4
v% A% Mortality:
%S ft Mean Offs rin eFmale:
I'S
-LI
[ ",1.Lt-600 NaCI/L Survival and Reproduction Data I ~" Replicate number ____ ___
Day' 1d 2 3 4 - 6 [ 8 9 10 Adult mortality _N _7 17 2 Youngproduced N Adult mortality jJ I .LIt ol b- o (l.s lo brodI )o.. *I() Q.
I s.p.li b--1 t1i Io Young producedl tr r 1 10 0 Ins0 I 60 mVug Day I_NC/
Admlt mortality 1
LFWT*.
1__ J Iq314 JC]--
uvvladRproducto
- I *t , 1[T7I* Data:* I *'- 8_ I- 9 I 1' 10
,4 l__YoungAdult produced IA~
I Adult mortality mortality 2 Young produced i~ SL 4(n I
1'-.
- -0II 113"1
', I I L- II*"I* ['Lr 'I%
1*I L *" L I Mot ality L.._ [107.
L L i-iJ.n
~~ 1 Adultmortallty 'I__l. .f. JMe [ 1+/-1 Toa Young produced 1 .0~3b 2 .20 It Young produced j oi . 0 "5 -- 3 0 1 Final Adult Mortality .. . TJ - t. ,.... ... t.
Note: Adult mortality (L = live, D dead), SB = split brood (single brod split between two days), CO = carry over (offspring L carried over with a dluring ansfer).
Concentration:
% Mortality: 07.
Mean Offspring/Female: 0o.2-.
% Reduction from Control: -.a 7.
SOP AT14 - Exhibit ATI4.1, revision 06 11
%p ge 91 of 99
Page 3 of 6 6ETS Species: Ceriodaphniadubia CdNaCICR #: I_&
800 ma NaCI/L Survival andReproduction Data Replicate number Da y 1 2 3 4 5 6 7 8 9 10 I Young produced 0 0.. 1) 0 0C1 0
_ Adlmortality[ ~ N.- _ I~
2 Young produced Adult mortality _ __ .
- a . .. . ',. s..
3 oung produced Adult mortality __,
0 )
- I I I ___________________________
0J O0 01 0 4 Young produced "%I t, Adult mortality S Young produced ~I~Ii 103J 17-1 06I__ 2. C Adult mortality " %._ _ .... *q-.
6 oung produced __ __S_ _
Adult mortality _-_-
- _
- I I 7 Young produced -
Total young produced . "%..w z B0% 'Lt Final Adult Mortality X__ _.. . -%._ j_ _ .
Note: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), CO = earry over (offspring carried over with adult during transfer).
Concentration:
%Mortality: 07.
Mean Offspring/Female: 30A
% Reduction from Control: k17.-
1000 mg NaCI/L Survival and ReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 1 10 1 Young produced ] , - I- - - i - -
Adult mortality t,.-*_ - " _ _ I '
nalAdult mortality.L L I U-.- UI. L,-
3f Young produced C) I e) Cp oe Adult mortality W tL I IroLrIoLspItw w rr i a4 Young produced*
Adult mortality JL s6 JA .~J .J...
I I
%-~1 I 2oa Young produced o* n} b 6 **"- '" * **
5 Young prdue 0C 1_ '1,t 10 IP I1.
[Adult mortality V_ _ .- . _ _ _ _ _ _
.6 Young produced ti 0. 0 0 0 ' __
Adult mortality X4_ I__LJ__%_I '- I V-- I -I %-
7 Young producedJ %S 'S ~ IS '- 16 17'.
i- IS, Final Adult Mortality 7-k L -'
Note: Adult fliortality (L = live, D =dead), SB =split brood (single brood split between two days), CO =carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality: 10.q Mean Offspring/Female: 24.q I% Reduction from Control: ' .'I SOP AT14 - Exhibit AT14.1, revision 06-01-11
- age 92 of 99
Page 4 of 6 0ETS Species: Ceriodaphniadubia CdNaCICR #: _
1200 me NaCI/L Survival andReproductionData Replicate number 8 9 10 5 6 7 1 Young produced Q .3 Qt 4 III Adult mortality 1 1 Young produced 1 Adult mortality ___ A.--'-__ - -P I' 1_-'-
3 Yugpoue0- n 0_l
_J Adult mortality I~IC4CJ _it
,o I .- I I 4
5
__IAdult Young produced mortality oung produced U J I.*jZ*
I J'
I 1 2I I
I'- ' __
L j L ._
_ Adult mortality. I 1'".- ['- "FTIL-" -.-..
Adult mortality
- 7 Young produced 5 _ iI -- -1 %A Total young produced jA I1S 12- 66 1 16 Final Adult Mortality -Q- %? N %-- -t %.- %.- % ..-
Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
%Mortality: 0?.
- .Mean Offspring/Female: %.L
% Reduction from Control: SI'&.
1400 mg NaCI/L Survival andReproductionData Replicate number Day 1 1 2 3 4 5 6 7 8 9 10 1I Young produced Adult mortality I QL. .. L CL C E. t 1 2 Yongproduced cc) I () rr ia SAdult mortaliayt y* _I--I** I I I I L _
3 Young producedI 0. _ O I
'4 Young produced - * . .
Adult mortality 63 Young produced I~ IL t ~ 1.. 0.~
0J.JA
__ Adult mortality mortalityj I l IU .4UI. LI_
L IL L u,._
1 U L. j L.- ... i 7 Young produced _,,, _ . X 1 __-_
Total young produced Lk A 10S01 L j C) .
Final Adult Mortality _ * * * . . .
Note: Adult mortality (L = live, D =dead), SB = split brood (single brood split between two days), CO = carry over (offspring Concentration:
Mortality: 07.
Meann Offilspring/Female: 1,,.0 I% Reduction from Control: 71. $1.4 SOP AT14 - Exhibit AT14. 1, revision 06-01-11 age 93 of 99
!ETS Fnvlnrnmental Testing Solutions, Inc.
Verification of CeriodaphniaReproduction Totals Control 1000 mg NaCI/L 4Replicate number Day Replicate number Total Day Total 1
Dy 21 3 4 5 6 7 8 9 10 1 2. 3 6 7 8 19 1 T10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 5 5 6 4 5 5 47 4 5 4 6 4 4 4 4 3 4 4 42 5 11 0 10 10 10 10 12 10 10 10 93 5 0 12 11 11 10 10 10 10 10 10 94 6 0 10 0 0 0 0 0 0 0 0 10 6 11 0 0 0 0 0 0 0 0 0 11 7 15 13 15 15 14 15 13 16 13 13 142 7 15 15 13 15 12 14 13 13 13 14 137 Total 30 28 29 29 29 30 31 30 28 28 292 Total 31 31 30 30 26 28 27 26 27 28 284 600 mg NaCI/L 1200mg NaCLa Day ay 1 2 3 -4 Replicate 5 ,6 number 7 8 9- 1- oa Total Day Re cate number Total 1 0 0 0 0 0 0 0 0 0 I _ 1 2 3 4 5 6 7 8 9 10 0 0 12 00 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 .0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 6 6 4 6 5 5 5 51 4 3 1 2 2 3 2 1 3 3 3 23 5 11 0 10 10 13 11 11 10 13 10 99 5 7 0 6 4 5 8 5 6 9 5 55 6 0 11 0 0 0 0 0 0 0 0 11 6 0 9 0 0 0 0 0 0 0 0 9 7 14 13 14 14 1 1 4 13 13 141 7 4 6 5 3 5 2 7 4 4 5 45 Total 30 29 28 30 33 31 33 29 31 28 302 Total 14 16 13 9 13 12 13 13 16 13 132 800 iNg aCi/L 1400mni NaCIIL Re plicate number Total Day Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
3 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 4 4 5 4 6 6 5 48 4 1 1 1 2 2 3 2 3 3 1 19 5 11 11 10 11 10 12 10 13 12 10 110 5 0 0 2 3 0 2 4 1 2 2 16 6 0 0 0 0 0 0 0 0 00 0 6 1 3 0 0 2 0 0 0 0 0 6 7 14 14 14- 13 13 16 15 17 13 14 143. 7 2 2 1 1 1 3 2 3 1 3 19 301 Total 30 30 28 28 27 33 29 36 31 29 301 Total 4 6 4 6 5 8 8 7 6 6 60 File: CdNaCICR_080712.xlsx Entered by: J. Sumner Reviewed by:
CA 0
too Ceriodaphniadubia Chronic Reference Toxicant Test
- EPA-821-R-02-013, Method 1002.0 is Environmental Testing Solutions, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #138 Test dates: August 07-14,2012 Concentration Replicate number Survival Average reproduction Coeffident of Percent reduction from (mg/L NaO) 1 2 3 4 5 6 7 8 9 10 (%) (offspringfemale) varIatton (%) control (%)
Control 30 28 29 29 29 30 31 30 28 28 100 29.2 3.5 Not applicable 600 30 29 28 30 33 31 33 29 31 28 100 30.2 6.0 -3.4 800 30 30 28 28 27 33 29 36 31 29 100 30.1 8.9 -3.1 1000 31 31 30 30 26 28 27 26 27 28 100 28.4 6.9 2.7 1200 14 16 13 9 13 12 13 13 16 13 100 13.2 15.1 54.8 1400 4 6 4 6 5 8 8 7 6 6 100 6.0 23.6 79.5 Dunnett's MSD value: 1.929 MSD = Minimum Significant Difference PMSD: 6.6 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (I0 th percentile) = 13%.
Upper PMSD bound determined by USEPA (90'h percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200 1a; USEPA, 2001 b).
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICR_080712.xlsx Table populated from associated 'Verification of Cedodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Sumner Reviewed by: 1
Statistical Analyses SETS 6 nWrwrmWeazft oou~wwIm Ceriodaphnla Survival and Reproduction Test-Reproduction Start Date: 8/712012 Test ID: CdNaCICR Sample ID: REF-Ref Toxdcant End Date: 8/14/2012 Lab ID: ETS-Envlr. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Cariodaphnia dubla Comments:
Conc-mgIL 1 2 3 4 .6 6 7 8 9 10 D-Control 30.000 28.000 29.000 29.000 29.000 30.000 31.000 30.000 28.000 28.000 600 30.000 29.000 28.000 30.000 33.000 31.000 33.000 29.000 31.000 28.000 800 30.000 30.000 28.000 28.000 27.000 33.000 29.000 36.000 31.000 29.000 1000 31.000 31.000 30.000 30.000 26.000 28.000 27.000 26.000 27.000 28.000 1200 14.000 16.000 13.000 9.000 13.000 12.000 13.000 13.000 16.000 13.000 1400 4.000 6.000 4.000 6.000 5.000 8.000 8.000 7.000 6.000 6.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc.mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 29.200 1.0000 29.200 28.000 31.000 3.537 10 29.833 1.0000 600 80.200 1.0342 30.200 28,000 33.000 6.005 10 121.00 75.00 29.833 1.0000 800 30.100 1.0308 30.100 27.000 36.000 8.921 10 111.50 75.00 29.833 1.0000 1000 28.400 0.9726 28.400 26.000 31.000 6.884 10 92.00 75.00 28.400 0.9520
'1200 13.200 0.4521 13.200 9.000 16.000 15.067 10 55.00 75.00 13.200 0.4425
'1400 6.000 0.2055 6.000 4.000 8.000 23.570 10 55.00 75.00 6.000 0.2011 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.17686 1.035 0.53863 0.89662 Bartlett's Test indicates equal variances (p = 0.14) 8.40619 15.0863 Hypothesis Test (1-tail. 0.05) NOEC LOEC ChV TU Steers Many-One Rank Test 1000 1200 1095.45 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point ma/L SD 95% CL Skew IC05 1000.77 44.7051 868.009 1015.87 -1.6465 IC10 1020.39 9.61319 991.422 1034.38 -1.3975 IC15 1040.02 7.68096 1020.26 1053.25 -0.3510 1.o-IC20 1059.65 7.19436 1041.68 1071.64 -0.3336 0.9 IC25 1079.28 6.83735 1063.08 1090.45 -0.3157 1138.16 6.71529 1123.99 1148.74 -0.2616 0.8 IC40 IC50 1177.41 7.42545 1162.25 1189.63 -0.2048 0.7 0.6 0.4 0.3 0.2 0.1.
0.0 ------
-0.1 1 0 500 1000 1500 Dose mg/L Dose-Response Plot 30 25 20 10 5
File: CdNaCICR0807I2.xlsx Entered by: J. !;unmer Reviewed by. _-_
(fage 96 of 99
"ETS (a Environmentl Tesoti ons, Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 8/7/2012 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 8/14/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Used for PMSD calculation only.
Conc-maiL 1 2 3 4 6 6 7 8 9 10 D-Control 30.000 28.000 29,000 29.000 29.000 30.000 31.000 30.000 28,000 28.000 600 30.000 29.000 28.000 30.000 33.000 31.000 33.000 29.000 31.000 28.000 800 30.000 30.000 28.000 28.000 27.000 33.000 29.000 36.000 31.000 29.000 1000 31.000 31.000 30.000 30.000 26.000 28.000 27.000 26.000 27.000 28.000 1200 14.000 16.000 13.000 9.000 13.000 12.000 13.000 13.000 16.000 13.000 1400 4.000 6.000 4.000 6.000 5.000 8.000 8.000 7.000 6.000 6.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 29.200 1.0000 29.200 28.000 31.000 3.537 10 600 30.200 1.0342 30.200 28.000 33.000 6.005 10 -1.186 2.287 1.929 800 30.100 1.0308 30.100 27.000 36.000 8.921 10 -1.067 2.287 1.929 1000 28.400 0.9726 28.400 26.000 31.000 6.884 10 0.948 2.287 1.929
- 1200 13.200 0.4521 13.200 9.000 16.000 15.067 10 18.969 2.287 1.929
- 1400 6.000 0.2055 6.000 4.000 8.000 23.570 10 27.505 2.287 1.929 AuxillaryTests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.17686 1.035 0.53863 0.89662 Bartletts Test indicates equal variances (p = 0.14) 8.40619 15.0863 Hypothesis Test (1 -tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob CIf Dunnett's Test 1000 1200 1095.45 1.92879 0.06605 1109.51 3.55741 1.6E-38 5,54 Treatments vs D-Control File: CdNaCICR_0807 [2.xlsx Entered by: J. Sumner Reviewed by: _A-
- age 97 of 99
.. . ...I.,. .:.e.5
,.:6 Page 5 of 6 Species: Ceriodaphniadubia CdNaCICR #: IVb Daily Chemistrv:
- * "Day (Aayst identified for each dayr performed pHl, D.O. and conductivity measure'ments only.
Concentration Parameter PH (s.u.) [
Conductivity (gmihos/cm) 0 CONTROL *Alkalinity (Ing CaCO3/L)
- Hardness (mg CaCO 3 L)"
- Temperature (*C). ' " *A*. . 1¢4. 'i -. 1 pH (S.U.) 1.7C .23 I _"
DO (mg/L) 7.7 9" D 600 mg NaCIIL Conductivity (tuhhos/cm) 14t__'_13_
_ _ _ *Temperature(*C) -Lq A U_0 , . . C) pH (S.U.) M.U 147 DO (mgIL) 7.1 800 mg NaCI/L Conductivity
- Temperature (9C) 1I4.0, .0 1. 0 all pH (S.'U.) -7.77____
1000 mg NaCI/L Conductivity
pH (S.ff.) "7,77/ f .*4 I**Z 7.*7.S 0 ' *. 8._
1200 mg NaCI/L Conductivity I *Temperature (OC) 75,.0 . ..- .1., I S. .
pH (S.U.)
. * ~ I- 7.90 "4.91 DO- (mg/L) 1400 mg NaCIIL Conductivity ' o (g+/-mhos/c) ________
- Temperature (SC)
STOCK Conductivity t
_ _ 1~ -gno/m Initial I1 1
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
-em.-,
b~,wby Kt.,EKnm SOP AT14 - Exhibit AT14.1, revision 06-01-11 e 98 of 99
S Page 6 of 6 S0ETS E~maIT~OgSOI**b~
Species: Ceriodaphniadubia CdNaCICR #: I b t" Day (Analyst identified for each day, performed PK-D.O. and conductivity measurements on l,.
3 4 5 6 4Analyst zPl orBMjoa Concentration Parameter pH (S.u.) "-CS 7.S 1.S _. _ _ . .S .5 . 7.i*'t.
DO (mg*L) 77 0 -. 7..
Conductivity
- Alkalinity (tunhos/cm)3o31 CONTROL *Aklinity (Ing CaCO3/L) t 2
- Hardness (Ing CaC03/L) 0.0
- Temperature " " -o A 'L L .Lblu aAAVi.A .0.*\
DO (mg/L) 7.q S600 mg NaCIIL Conductivity I~)___ __
t
- Temperature ..* j '*z.. 8, pH (S.U.) " - .75 -7.'06 "___ 196 .2.1 . 2.+/-*I -z.
DO(mg/L) , .o -. q 2 ._
800mg NaCI/L Conductivityo ,o m gmhos/cm _
- Temperature "q* "$ S LO "t,* *7:t "*" L, I)MY _T_
pH (S.U.)
/1 -1P U *-'E~-4-'----
I .7.-77 II -- 4 I 7.93
'7.2- 4 It 1 -7 1~ 1. -1. 1 4I-.L~~'---4 A.n M.Z .11 7.7"L DO (ma/L) W-4 11 1000 mg NaCI/L Conductivity (jImhostcm) r1 __6 _0 i 03O
- Temperature (00) -0.1 11-LS. 1) "U.0 u1.5 11% S.%I pH (S.U.) ," "7.2I ".Lq 811." 7.7' .. ".Z 7.7 DO (rag/L) * . . . 7= , )-* 7.
1200 mng NaCL/L Conductivity (ginhos/cmn) 9141
- Temperature ""
.. PH (S.U.) 13-- ". $ /q
-7* 7.q1"j { '* * . '
DO (mg/L). , 7.q 8.o 1400 mg NaCIIL Conductivity
- Temperature "** .,L . Lq.* '.* -Let -Lq. "T.. 1 1
, ¢CIL Initial Final I-U Initial 11 a Final Ir Initial U 11 Final m
I Initial 11 Final .0 I
- Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:
SOP ATI4 - Exhibit ATI4.1, revision 06-01-11
- Z. ge 99 of 99