Text

Discharge Monitoring Report for May 2011
	ML11160A101
Person / Time
Site:	Sequoyah
Issue date:	06/08/2011
From:	Skaggs M; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML11160A101 (109)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MAY 2011 Enclosed is the May 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection at the Diffuser Pond Inlet of the Yard Pond effluent due to a transformer oil spill that reached the Yard Drainage Pond has been discontinued as of 5/14/2011. Samples collected from 9/23/2010 through 5/14/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.

I certify under penalty of law that this document and all attachments were preparedunder my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significantpenalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

Sincerely, Michael D. Skaggs Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved.

MAJOR DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0C104 Address P.O. BOX 2000 (INTEROFFICE OPS-ELN-SN) TNO026450 J10G F - FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUMBER- DIFFUSER DISCHARGE FacilitY VA - SEOUOYAH NUCLEAR PLANT F EFFLUENT Locatio HAMILTON COUNTY- I MO MONITORINr vrYI:AR I nAY PERIOD Froml 11 o05 1 01 ,I1 Tol IYE 11 RI 1 05M 311 *** NO DISCHARGE E ...

A1TN: Stephanie A. Howard NOTE: Read instructions before comoletino this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ** 35.7 04 0 31 / 31 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 RERMET Req. Mon. DEG. C. CONTI CALCTD EFFLUENT GROSS DAILY MAX NUOUS I TEMPERATURE, WATER DEG. SAMPLE

25.9 04 0 31 / 31 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT 30.5 DEG. C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAIL.NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2 04 0 31 / 31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S PERMIT 3 DEG. C. CONTI CALCTD REQUIREMENT EFFLUENT GROSS I. .. DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1644 03*** 0 31 / 31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT

Req. Mon. MGD -****CONTI RCORDR EFFLUENT GROSS REQUIREMENT-DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE 0.023 0.046 19 0 21 /31 GRAB MEASUREMENT 50060 1 0 JPERMIT * .1 .01 MGIL FIVE PER CALCTD EFFLUENT GROSS MOAVG DAILYMAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0 62 0 31 / 31 CALCTD CHANGE MEASUREMENT 82234 1 0 PERMIT . DEG CONTI CALCTD EFFLUENT GROSS REQUIREMENT AIC/HR -2DECNUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

-irection or supervision in accordance with a system designed to assure that qualified personnel

'-, TELEPHONE DATE Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE TYPED________

OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTEDOFIEORATOIEAEN ARA NMR YAR O DY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceall attachmentshere No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Towerbrom 960 2. Floguard MS6236 (max. calc. conc. was 0.1lmg/L--Iimit 0.2mg/L) 3. Spectrus CT1300 (max. calc. conc. was 0.039mg/L--limit 0.050mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

Mean # of WaterWaeSU Mean# of Water SUB NOTES: %

Sample Date ZM/m3 %Settlers Temp. (°C) Sample Date Asiatic Temp. (°C) LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 6 100 23 12/07/2010 0 23 1-25-545 PB 12/14/2010 0 0 10 12/14/2010 0 10 1-25-545 RS 12/22/2010 0 0 10.5 12/22/2010 0 10.5 1-ISV-24-1234 WE 12/29/2010 0 0 26 12/29/2010 0 26 1-25-545 WDT 01/04/2011 0 0 13 01/04/2010 0 13 1-25-545 PB 01/11/2011 0 0 22 01/11/2010 0 22 1-25-545 RS 01/18/2011 0 0 9.5 01/18/2010 0 9.5 1-ISV-24-1234 CR 01/25/2011 0 0 23 01/25/2011 0 23 1-25-545 WDT 02/02/2011 0 0 10 02/02/2011 0 10 1-25-545 PB

.02/08/2011 0 0 9 02/08/2011 0 9 1-25-545 MJW 02/15/2011 0 0 23 02/15/2011 0 23 1-25-545 MLW 02/22/2011 20 100 10 02/22/2011 0 10 1-25-545 PB 03/01/2011 0 0 11 03/01/2011 0 11 1-ISV-24-1236 PB 03/08/2011 0 0 11 03/08/2011 0 11 1-ISV-24-1236 WE 03/16/2011 22 0 11 03/16/2011 0 11 1-ISV-24-1234 MLW 03/23/2011 0 0 11 03/23/2011 0 11 1-ISV-24-1234 MLW 03/30/2011 0 0 12 03/30/2011 0 12 1-1 5v-24-1236 MLW 04/06/2011 18 100 15 04/06/2011 0 15 1-ISV-24-1234 HMW 04/08/2011 45 100 15.5 04/08/2011 0 15.5 1-1SV-24-1236 WAW/PB 04/20/2011 21 100 16 04/20/2011 0 16 1-1SV-24-1236 PB May 2011 No Samples Collected

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) 0MB No. 2040-0004 Address P.O.BO2000 (INTEROQFICE OPS-5N-SQN)

TN026450 10T F-FINAL SODDY - DAISY, TN 37384 PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HIAMILTN_ COUNTY MONITORING PFRIOD I EFFLUENT From[ 11 1 05 1 01 1 TOIF I osI 3 r YoARm MOA DAY IT NO DISCHARGE E]--- .

ATTN: Stephanie A. Howard NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE * >100.0 23 0 1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT 43.2 ******** PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT

.REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my - TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael 0. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site sident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED ORPRINTED I COD COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere Toxicity sampling began on May 1 and ended on May 6. The toxicity report is included.

EPA Form 3320-1 (REV 3/99) Previouseditions maybe used Page 1 of 1

June 3* 2011 Bradley M. Love, OPS 5N-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING. NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS. MAY, 2011 Per your request, I have also submitted an electronic copy of the subject report. Outfall 101 samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids.

The resulting IC2 5 values for both species were> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since lish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV-treated samples.

Please call me at your convenience if you have any questions or comments following your review of the report.

Donald W. Sno grass Manager (Acting),

Environmental Engineering Field Team-Muscle Shoals CTR 2L - M Attachment Cc (Attachment):

R.M. Sherrard, PSC IX - C (Electronic copy)

EDMS, MPB 1E - M (Electronic copy)

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: June 03, 2011

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 3,491

8. Outfall Tested: 101

9. Dates Sampled: May 01 - 06, 2011

10. Average Flow on Days Sampled (MGD): 1635.7, 1623.12, 1618.58

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: May 03 - 10, 2011

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphniadubia)

15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC 5 = 43.2%

17. Test Results: Outfall 101: Pimephalespromelas:I__ .> 100%

Ceriodaphniadubia: IC 2 > 100%

UV treated Outfall 101: Pimephalespromelas: IC 25 > 100%

Page 1 of 100

18. Facility

Contact:

Brad Love Phone#: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #:. (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Outfall 101 samples collected May 01 - 06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 2 of 100

.METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (°C) (mg/L) Last Used By 05-01-11 0800 to 05-03-11 1415 101 05-02-11 0700 05-02-11 1512 0.7,0.8t <0.10 05-04-11 1318 05-01-11 0845 to 05-03-11 1415 Intake 05-02-11 0745 05-02-11 1512 1.2 <0.10 05-02-11 0745 05-04-11 1318 101 05-03-11 0800 to 05-04-11 1437 0.7, 0. 9 t <0.10 05-05-11 1319 05-04-11 0700 05-06-11 1317 05-03-11 0830to 05-05-11 1319 Intake 05-04-11 0730 05-04-11 1437 0.8 <0.10 05-04-11 0730 05-06-11 1317 05-05-11 0800 to 0.9, 0.9, 05-07-11 1319 101 05-06-11 0700 05-06-11 1515 1.5 tt <0.10 05-08-11 1322 05-09-11 1316 05-05-11 0830 to 05-07-11 1319 Intake 05-05-11 0730 05-06-11 1515 0.9 <0.10 05-08-11 1322 05-06-11 0730 05-09-11 1316

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

tSamples were collected in two 2.5 gallon cubitainers and one 5 gallon cubitainer. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 + 1.0°C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 3 of 100

Pimephalespromelas Ceriodaphniadubia Test Organisms:

1. Source: Aquatox, Inc. In-house Cultures

2. Age: 21.75 - 22.25 hours2.893519e-4 days 0.00694 hours 4.133598e-5 weeks 9.5125e-6 months old < 24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 10

5. Organisms per Replicate: 1

6. Test Initiation: (Date/Tim Outfall 10 05-03-11 1415 ET 05-03-11 1315 ET UV Treated Outfall 10 05-03-11 1345 ET

7. Test Termination: (Date/]

Outfall 1011 05-10-11 1325 ET 05-10-11 1224 ET UV Treated Outfall 10 1 05-10-11 1342 ET

8. Test Temperature: Outfal 1101: Mean = 24.7°C Mean = 25.0°C (24.2 - 25.2 0 C) (24.7 - 25.2 0 C)

Test Temperature: UV-Tr *eatedOutfall 101: Mean = 24.80 C (24.2 - 25.2 0 C)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 03 - 10, 2011 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(%Effluent) 1 J 2 3 4 [ 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 98 95 93 Mean Dry Weight (mg)

Test Solutions (replicate number)

(%Effluent) 1 2 3 4 Mean Control 0.704 0.756 0.799 0.819 0.770 10.8% 0.849 0.889 0.898 0.768 0.851 21.6% 0.659 0.886 0.913 0.879 0.834 43.2% 0.761 0.844 0.841 0.853 0.825 86.4% 0.845 0.805 0.716 0.797 0.791 100.0% 0.801 0.696 0.863 0.800 0.790 Intake 0.893 0.794 0.774 0.660 0.780 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.2%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = IO0/LC50 : TUc = 100/IC25 Page 5 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 03 - 10, 2011 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions -1 F2 3

(% Effluent)

Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Test Soluetis Data (replicate number)

(%Effluent) 1 2 3 4 5 6 7 1 8 9 o 10 Mean Control 28 28 31 31 31 31 30 29 29 29 29.7 10.8% 33 30 34 32 27 33 30 28 30 32 30.9 21.6% 33 32 31 36 29 32 31 31 31 33 31.9 43.2% 36 32 34 30 35 36 35 34 32 34 33.8 86.4% 34 35 33 35 36 37 36 35 33 35 34.9 100.0% 38 36 36 37 35 38 36 36 36 36 36.4 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.2%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC 50 : TUc = 100/IC 25 Page 6 of 100

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 03 - 10, 2011 using water from Intake Percent Surviving Test (time interval used - days)

Solutions 1

(% Effluent) 1 _2 3__56_

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Tet Soluetis Data (replicate number)

(%Effluent) 1 2 3 4 5 6 17 8 19 110 Mean Control 29 26 34 30 29 27 30 32 31 29 29.7 Intake 37 37 35 35 37 35 32 36 38 36 35.8 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50 : TUc = 100/IC 25 Page 7 of 100

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 03 - 10, 2011 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 [ 2 ] 3 4 1 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 98 98 98 86.4% 100 100 100 100 98 98 98 100.0% 100 100 98 98 98 98 98 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg)

Test Soluetis (replicate number)

(%Effluent) 1 2 3 4 Mean Control 0.714 0.908 0.853 0.849 0.831 10.8% 0.901 0.813 0.867 0.888 0.867 21.6% 0.896 0.793 0.779 0.829 0.824 43.2% 0.879 0.852 0.847 0.773 0.838 86.4% 0.850 0.770 0.774 0.797 0.798 100.0% 0.816 0.802 0.660 0.840 0.780 Intake 0.948 0.883 0.925 0.799 0.889 IC 2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0 : TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species F Date Time Duration Toxicant Results IC 25)

Pimephalespromelas May 03 - 10, 2011 1445 7-days KC1 0.78 g/L Ceriodaphniadubia May 03 - 10, 2011 1255 7-days NaCl 1.07 g/L Page 8 of 100

PHYSICAL/CHEM ICAL SUM MARY Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphniadubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 03-10, 2011.

Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (tmhos/cm) (mgfL CaCO 3) (mg/L CaCO3 ) Chlorine (mg/L) 24.8 24.6 7.6 7.7 7.81 7.77 321 59 90 Control 24.7 - 24.9 24.4 - 24.8 7.4 - 7.7 7.5 - 7.9 7.73 - 7.89 7.68 - 7.86 305 - 333 58 - 59 89 - 91 24.6 7.8 7.7 7.85 7.73 299 10.8% 24.9 24.7 - 25.0 24.2 - 24.8 7.5 - 8.2 7.3 - 8.0 7.80 - 7.93 7.64 - 7.87 283 - 311

%) 21.6% 24.9 24.6 7.9 7.7 7.85 7.73 282 24.7 - 25.0 24.4 - 24.8 7.7 - 8.1 7.3 - 8.0 7.79 - 7.93 7.64 - 7.86 272 - 292 Q. 43.2%/ 24.9 24.6 7.8 7.7 7.83 7.73 249 24.7 - 25.0 24.5 - 24.8 7.7 - 8.1 7.4 - 8.1 7.77 - 7.92 7.66 - 7.86 241 - 257 86.4% 24.9 24.5 7.8 7.7 7.80 7.71 188 24.7 - 25.1 24.4 - 24.6 7.7 - 8.0 7.4 - 8.1 7.72 - 7.92 7.66 - 7.85 179 - 207 25.0 24.5 7.9 7.7 7.79 7.71 163 58 63 < 0.10 100.0%

24.9 - 25.2 24.3 - 24.7 7.7 - 8.1 7.4 - 8.0 7.71 - 7.90 7.64 - 7.85 157 - 174 54 - 61 55 - 71 <0.10 - <0.10 24.4 7.9 7.7 7.78 7.70 164 59 62 < 0.10 Intake 24.9 24.7 - 25.1 24.3 - 24.6 7.8 - 8.1 7.3 - 8.0 7.69 - 7.92 7.64 - 7.87 155 - 178 55 - 61 55 - 67 <0.10 - <0.10 24.9 25.0 7.6 7.7 7.81 7.84 321 59 90 Control 24.8 - 24.9 24.7 - 25.2 7.4 - 7.7 7.4 - 8.0 7.73 - 7.89 7.77 - 7.90 305 - 333 58 - 59 89 - 91 25.0 7.8 7.7 7.85 7.82 299 10.8% 25.0 24.9 - 25.0 24.8 - 25.2 7.5 - 8.2 7.5 - 8.0 7.80 - 7.93 7.71 - 7.90 283 - 311 26 25.0 25.0 7.9 7.8 7.85 7.84 282 24.9 - 25.0 24.8 - 25.2 7.7 - 8.1 7.6 - 8.0 7.79 - 7.93 7.77 - 7.91 272 - 292

. 43.2% 25.0 25.0 7.8 7.8 7.83 7.84 249 24.9 - 25.0 24.8 - 25.1 7.7 - 8.1 7.6 - 8.0 7.77 - 7.92 7.76 - 7.90 241 - 257 25.0 25.0 7.8 7.8 7.80 7.84 188 24.9 - 25.1 24.8 - 25.2 7.7 - 8.0 7.6 - 8.0 7.72 - 7.92 7.74 - 7.93 179 - 207 25.1 25.0 7.9 7.8 7.79 7.83 163 58 63 < 0.10 100.0%

24.9 - 25.2 24.8 - 25.2 7.7 - 8.1 7.5 - 8.0 7.71 - 7.90 7.72 - 7.93 157 - 174 54 - 61 55 - 71 <0.10 - <0.10 24.9 24.9 7.9 7.8 7.78 7.82 164 59 62 < 0.10 Intake 24.9 - 25.1 24.8 - 25,2 7.8 - 8.1 7.6 - 8.1 7.69 - 7.92 7.71 - 7.91 155 - 178 55 - 61 55 - 67 <0.10 - <0.10 Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.7 24.2 25.2 Ceriodaphniadubia 25.0 24.7 25.2 Page 9 of 100

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephalespromelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 03-10, 2011.

Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO3) (mg/L CaCO3 ) Chlorine (mg/L)

Control 24.9 24.6 7.8 7.7 7.83 7.74 311 59 92 24.8 - 24.9 24.4 - 24.9 7.5 - 8.1 7.5 - 8.0 7.78 - 7.91 7.66 - 7.84 299 - 326 57 - 60 90 - 96 24.6 7.8 7.7 7.85 7.75 297 10.8% 24.9 24.9 - 25.0 24.3 - 24.8 7.7 - 8.1 7.3 - 8.0 7.79 - 7.92 7.66 - 7.85 286 - 310 25.0 24.6 7.8 7.7 7.86 7.74 283 24.9 - 25.1 24.2 - 24.8 7.7 - 8.1 7.3 - 7.9 7.80 - 7.93 7.66 - 7.86 270 - 293 25.0 24.6 7.9 7.6 7.85 7.73 250 43.2%

24.9 - 25.1 24.5 - 24.7 7.7 - 8.1 7.3 - 7.9 7.79 - 7.91 7.65 - 7.86 240 -. 256 86.4% 25.0 24.6 7.9 7.7 7.83 7.71 187 24.9 - 25.1 24.5 - 24.7 7.8 - 8.1 7.4 - 8.0 7.75 - 7.90 7.62 - 7.87 178 - 197 100.0% 25.1 24.6 8.0 7.7 7.81 7.69 164 57 69 < 0.10 24.9 - 25.2 24.5 - 24.7 7.9 - 8.1 7.4 - 7.9 7.73 - 7.91 7.62 - 7.86 154 - 178 52 61 63 - 76 <0.10 - <0.10 Intake 25.0 24.6 8.0 7.8 7.79 7.70 164 58 69 < 0.10 24.8- 25.1 24.4 - 24.7 7.9 - 8.2 7.5 - 8.0 7.71 - 7.90 7.63 - 7.85 155 - 175 52 - 63 63 - 76 <0.10 - <0.10 Overall temperature (C) Average Minimum Maximum

.24.8 Pimephalespromelas 24.2 25.2 Page 10 of 100

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 01 - 06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 11 of 100

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephalespromelas Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphniadubia Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephalespromelas None

2. Ceriodaphniadubia None Page 12 of 100

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 2510 B.

7. Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821 -R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephalespromelas.

Sodium Chloride (NaCl crystalline) for Ceriodaphniadubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 100

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82 l-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 2 1st Edition, 2005.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 100

Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 100

Table B- 1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 6, 2011 Date Sddium Towerbrom PCL,-222,7 PCL-401 Ct3-63 Cuprostat- H-Hykpfochk)Fite mg/L flgL iu ~ mg/L mg/ii,,L PF 1:t1'mL L ?: TRC Plho itiat Copolymer DMAD mg/L (

Azole 03/12/1998 '0.016W, 3/4 K -

03/13/1998 0. 5.015 03/14/1998 y-O.01..

03/15/1998 0~t.03()~

03/16/1998 ,0.013 -

03/17/1998 'i 0.0201 03/18/1998 <>,0.018 - _ -

09/08/1998 0.01 .. *..... 0014 0.005 0 09/09/1998 0.003,, ( 31 1 0.011 09/10/1998 0.0142 - 0.)00d 0.021 09/11/1998 0.013 0.019 09/12/1998 0001 - 040.015 09/13/1998 ZO.0 1 0.015 09/14/1998 0.008, - 0,0'44 1 0.015 1 02/22/1999 <-O... 0 -

02/23/1999 *, :O005l * . -

02/24/1999 0.009 02/25/1999 &* 0.0 12 -

02/26/1999 0.008 - i.

02/27/1999 < -

02/28/1999 1 -.

08/18/1999 0.015 0100W9 0.024 08/19/1999 Ž>! KWi 0.012 0> ..068 0.024 08/20/1999 '<0.023 >0.070 0.024 0.120 08/21/1999 t 0.022

0.068 0.024 -

08/22/1999 , . 0.022 O6 '-x 0.024 -,-*--t-08/23/1999 "- 0.025 (.0068 0.024 9006 08/24/1999, 0.016 q. 7 1 0.023 0020.(

Page 16 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date *, Sod iu Towerbrom PCL-2222 PCL-401 1-36o3 Cuprostat- Hf-13*M Hpochiar-ite: mg/L m gL m I g-IVTC Pbm/ PF i/

sae Copolymer tDMAD mg/L Quat TR, Azole

)1/31/2000 < 0.002 0.026 0.009 . -

)2/01/2000 0.011 0.026 0.028 -

)2/02/2000 0.028 0.026 0.009 *0.006 -

)2/03/2000 0.008 0(0)7.2 0.009 -

)2/04/2000 0.006 0.027 0.009 00.109

)2/05/2000 < 0.002 0. 0.009 K. -

)2/06/20001 < 0.002 0,.027 1 0.009 --1[-

)7/26/2000 < 0.0057 0 055 0.019

)7/27/2000 0.019 0055 0.019 -...

)7/28/2000 <0'0080.0088 0505'0 0.018 0:001()4 0.108

<<7/28/2000 0.0088 0505 0.019 -

27/30/2000 <0.0076 ().() 0.019

27/31/2000 < 0.0152 ,0255 0.019 0.006 28/01/2000 < 0.0141 *0.025 1 20 0.019 .00.

[2/11/2000 0.003 0.020 0 12/12/2000 0.0092 O 0.020 ..000 .

12/13/2000 < 0.0120 0.06

0.020-005 12/14/2000 < 0.0087 01.06 i 0.0210.

12/15/2000 0.0120 ()12 , 0.020 >000:5 I0,021 12/16/2000 < 0.0036 0 0.020 *O.,05 _

12/17/2000 < 0.0036 1 0,025 0.020 18/26/2001 ,, 0.017 00.021 ,0 18/27/2001 - <0.0096 0024 0.021 0.005 - -

18/28/2001 <0.0085 0023 - 0.021 0.07 18/29/2001 -: <0.0094 50.059 0.020 0.011 6 8/30/2001 <0.0123 0.02* : 0.021

28/31/2001* 1 <0.0052 0590 0.020 11/25/2001 - <0.0044 i - -

11/26/2001 <0.0119 ().W4 0.02 21/27/2001 112/01'*>?' !! 0.0137

}<0.0089 0.024 2 0.019 0.019 ,0.00 iO'HO 11/29/2001 ,<,,,a+ *<,,* <,,,:,:,, 0.0132 ().(024 0.02 !~ n 1/30/2001 < 0.0043 0024 0.02 -

.2/09/2001 12/10/2001 <0.0042

<0.0042 ,"=' 0,* } 0.02 "0.00,"' . ..

.2/I11/2001 * '{* i<0.0104 * - ?ir

,2/12/2001 o, 0.0128 0.02: 04.o.00 12/13/2001 <0.0088 0",'024 0.02 i00Y*

Page 17 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Sodium Towerbrom PL-222 PCL-401 CL-1363* Cuprostat- iH-.130N/

Hypochiorite mg/L mg/

i . mg/L mlýI~ PF 111,g1 L

mg"L TRC spnate: Copolymer DMAMY mg/L Qii"t TRC Azole

)1/02/2002 < 0.0079 0023 0.02 0J6 -

)1/03/2002 <0.0042 0.014

)1/04/2002 0.0124 2 0024 0.014 *00

)1/05/2002 <0.0042 -

)1/06/2002 * <0.0042

)1/07/2002 - < 0.0089 0.024 0.014 o6i006 -

)2/24/2002 - < 0.004 . - - - -

)2/25/2002 - <00.004 0023W-) 0.023 2 !\. -

)2/26/2002 "00143 °0. *023' 0.023 _;0:007'

)2/27/2002 < 0.0041 *0023 0.023

)2/28/2002 < 0.0041 0.024 0.008 -

)3/01/2002 < 0.0041 0.024 1 0.008

)5/05/2002 -'""""* } ... .. - ..- !

)5/06/2002 0080.02 0.014

)5/07/2002 05 0.02 15,

)5/08/2002 0.0 0.019 -

)5/09/2002 (0.057 0.02 WO-14

)5/10/2002 1 - (0.056 0.019 .

)8/04/2002 " - <0.0058 - -018

)8/05/2002 - <0.0058 0.053 0.018 -0.025

)8/06/2002 i 0.0092 0530.018

)8/07/2002 - , <0.0107 0.0719 0.0().055

)8/08/2002 * <0.0061

0:055 0.019 --

)8/09/20021 1 0.0152 O054*5 0.018 0.008

,0/06/2002 - <0.00497 .

0/07/2002 0.0153 0.054 0.018

.0/08/2002 - <0.0092 0.054 0.018 O0007 10/09/2002 - 0.0124 ;i:0 053 0.018 0w009 10/10/2002 0.0134 0. 0 4 0.018 000 10/11/2002 1 <0.0042 . 0..04 0.018 K

)1/12/2003 4" <0.0035 01/13/2003 @ i. , <0.006 0'025 0.019 0009

)1/14/2003 * - <0.0118 (),

0 26, 0.020

)1/15/2003 @ <0.0063 0.026 0.020 0

)1/16/2003 <0.0034 0026 0.020

)1/17/2003 <0.0034 1 0.026. 0.009

)4/06/2003 - - <0.0073 ..-....

)4/07/2003 <0.0189

0.021 , ,

)4/08/2003 -<0.0 117 0.02 1

)4/09/2003 * <0.0139 0.021 0*016 ,

)4/10/2003 @ <0.0113 0.021 0018

)4/11/20031 <0.0073 1 0.022 Page 18 of 100

Table B- I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date [ SodlSdnl Towerbrom +PCL2-2*"' PCL-401 A.-. 363 Cuprostat- H,11<30O 1yOC ilt mg/L mI-*,L I' mg/L m./L PF mg/I IIm,-

gL TRC Posphat*e Copolymer ::DMAF mg/L . Qua RC. : Azole 06/15/2003 [* <0.0045 -l- .

06/16/2003 < 0.0037 .... 0.020 - 0,02 06/17/2003 . < 0.0048 0041 0.014 - 0t2, 08/06/2003 <<0.0084 0.041 0.0200 16/10/2003 < 0.0108 0.268 0.024 00092 06/20/20034 1 < 0.0048 0.027 1 0.0209 0,02ý 08/03/2003 <0.0034 5 0*0'" 0.009 02/03/2004 <0.0050 1) 0.0209. +

08/04/2003 0.0<051 0.006k 0.00*92:.009 -

08/05/2003 <0.0034 1-) 2657 0.020 002:

08/06/2003 0.0105 0 , 0.0209 0n0n0 05/04/20041. <0.0123 0 026.019.... 0.020 05/05/2003 <0.0104 ").0572 2 0.020 05/06/2003 <0.0143

<* (0.057 0.020 -0.02' 0 10/07/2003 <0.0022 ,0058 0.020 0.0 -2 10/08/2003 <0.0106 0.67 0.020 02 10/09/20034 ... <0.0104 0 0.020 - 0, 0 10/10/20041 0.0187 0.02 0.019 0.00 02/01/2004 - 0.0093 0.057 0.009 0 ...

02/02/2004 - <0.0034 0(

.058 0.009 02/03/2004 - <0.0034 0.05-616 0.009 -0 02/04/2004 - 0.0124 0.020 0.009 0.009 - -"

07/09/2004 - <0.0034 (0.057 0.009 0 ',

02/06/2004 -0.0105 70.020 0.009 H'010+*+

05/04/2004 -li7i

@:!*,7 <0.0123 i0.0,26+ 0.019 ,

05/05/2004 - + <0.0144 *0.026 0.014 (0(9*+NO_

05/06/2004 -++*++++ <0.0146 +,':0.0......"-'! 0.013 . .. ,!?.-'*=.......

05/07/2004 ......,N -+ 0.0227 0.:058 0.020 fff-0"),,02 05/08/2004 -0.016 *0, 0+60+ 0.021 N 05/09/2004 <0.0104 (0!J)58 0.020 07/04/2004... 0.0217- + +n.+: +7 0.019 ,,-+-

07/05/2004 ++++++: +++ <0.008 .... 0+ný;- 0.020 H.00-07/06/2004 i;:::2 :5:Z*<0.0077 0,0ýý0.020 .. i~i*'

07/07/2004 ":::::0.0252 5<u 6* 0.019 X 07/08/2004 , 0.0223 ý- 0.019 W~~slo111 07/09/20041 1 0.0182 o.0ý7" 0.020 (.*60)(H Page 19 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Sodium Towerbrom ll 222 PCL-401 CL-163 Cuprostat-PF ýH-i30M Nalco Hypochlirit mg/L mg1,2 1 mgIL ~ gL mgIL mgLI'L 73551 e TRC Phlsph*atcý Copolymer m) %I..A1 Azole *:,*t mg/L zEo/Po

<0.0187 0.00() 0.014--

, .** <0.0192 0NO.04'7, 0.030

<0.0233 0,048 0.016 0,04i

<0.0149 0,.:,47 0.016 (o 1

<0.0149 0i049)iig 0.017 OAO" .

~<0.0253 .. ......... ,, 0.017 0.4

<0.0042 0.010 S <0.0116 .0,28' 0.010 0.007

<0.0080

O028 .i >V 0.010 A..-

0.0199 0.0 0.010

<0.0042 0.,02.8 0.010 :

.0 0.0155 0&* 028 0.010 { " 0.007 0.0063 - ,

0.0043 S0.0103 0.0295 -

0.0129 -

0.0184 '-2l~i 0.0109 o.026 0.009 0.0150 0026 0.009 0.0163 o0.00 g-> 3 0.0209 002t4 0.009

.. 0.014

!*i0.0238 0.0200 OOS 0.009 0.018 ....- 0.014 i* !*!i*!*0.0104 t ..............

g4:i

v,*:}

0.0117 -. *i... .,

~0.0165 0.0256 :- -',-*

0.0116 *: ,:~g Page 20 of 100

Table B- 1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Towerbron PCL-401 Cuprostat-PF Nalco mg/L mg/L mg/L 73551 TRC Copolymer Azole mg/L EO/PO 11/12/2006 0.0055 11/13/2006 0.0068 11/14/2006 0.0143 11/15/2006 0.0068 11/16/2006 0.0267 11/17/2006 0.0222 11/26/2006 0.0188 11/27/2006 0.0138 11/28/2006 0.0120 11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/2007 05/29/2007 05/30/2007 0.0084 0.017 05/31/2007 0.0103 06/01/2007 0.0164 0.017 06/02/2007 0.0305 12/02/2007 0.0241 12/03/2007 0.0128 12/04/2007 0.0238 12/05/2007 0.0158 12/06/2007 0.0162 12/07/2007 0.0175 04/13/2008 0.0039 04/14/2008 0.0124 04/15/2008 0.0229 04/16/2008 0.0143 04/17/2008 0.0120 04/18/2008 0.0149 10/26/2008 0.0260 10/27/2008 0.0151 0.017 10/28/2008 0.0172 10/29/2008 0.0154 0.018 10/30/2008 10/31/2008 0.0086 Page 21 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Sodium Towerbror PCL- PCL-401 CL-363 Cuprostat H-130M Nalco ý[)pectrus Hil-iS5M MSW Hypochllori mg/L 2 mg/L mg/L -PF mg/L mg/L 73551 0T1300 mg/Lst 101 n ,L TRC mg/ L Copolymer DMA D Azole 2Quat t mg/L m4ng/L Quat mg/L Phopl EO/PO >~Quat Phosphate 02/08/2009 - 0.0197 0.017 02/09/2009 - 0.0237 ~ x- ~ -- 0.017 --

02/10/2009 - 0.0104 ,"- 0.021 -

02/11/2009 - 0.0155 I 0.017 02/12/2009 2 ..... .....-.- 0.0106 0.017 \

02/13/2009 , - .

05/10/2009 0.0129 05/11/2009 0.0-415 0.0446 05/12/2009 <0.010 0.0396 05/13/2009 0.0049 - - - N

0.0396 05/14/2009 - <0.0141 -...- - rit- 0.039, 05/15/2009 - <0.02160 . -

11/15/2009 . - 0.025 -0.0306t-11/16/2009 - 0.0152 -...- - : -

11/17/2009 - 0.0255 . .

11/18/2009 - 0.0306 ~

11/19/2009 & ~ 0.0204 11/20/2009 S< 0.0093 05/09/2010 ~ -~ 0.0192 05/10/2010 >~ 't 0.0055 05/11/2010 >c' 0.0100 - - 0.039) 05/12/2010 >'aK - 0.0171 - - 0.03,9 05/13/2010 0.0041 - - 0.03 05/14/2010 0.0099 --. - ;1.0391-0 Page 22 of 100

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Sdium o

Dae ~ oiiii;h Towerbro*-PC Towterbro 2 I2PCL*

PCL-401 !CL-363. Cuprostat H-130M PC/L mm/-.

Nalco 73551 Sp i-itI *H 50M MSW mg/L 222loc jmgL -PF mg/L n !L 73551 ('Tl309**mg

.I.I TRC mg/Li Copolymer DMAbD

Azole Q*A 7 mg/L 111!]- Quat 101 mg/L I R Ilisph EO/PO Qualit t Phosphate

~~< ~7ate7 10/31/2010 -

11/01/2010 0.0122 - ->- ,

11/02/2010 0.0112 . . - - -

11/03/2010 0.0163 -- - -

11/04/2010 - 0.0107 11/05/2010; - 0.0132- - -

05/01/2011I* -i- , -

05/02/2011 -: : - - - - I &7 4 -

05/03/2011 - - 004ý4 05/04/2011 .... 0.0155 - -

05/05/2011 - 0.0179 004 05/06/2011 - 0.0089 i-- - -

Page 23 of 100

Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 100

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NQ Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments:

Ray Duncan:

NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell :

Sara Snyder_

Collected By: RayDuncan, Ben Mtchell, Sara Snyder Fax: 828-350-9368 Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab.

l~eld Identification / Gab/Comp. CollectionDate/Time Container Flow g AL+C'1ýo8 Sample Description Number& MGD Rain Event? Laboratory Use Volume (Mark as Appropriate)

C ollected i: !*; : *,ii: i ii!**ii!:* *i:ii*!i-:*

DtTieYes if Yes, Inches No Trace ETS Log Number Anwial Temp.

~By T Time: Appear-ance SQN-101-TOX Comp ~ ,2 (2.5gal) I6Z- 7 .I - s y SQN-INTAKE- Comp al Sample Custody - Fill In From Top Down 4 , a-,. * . n Relinquished By (Signature): Date/Time Received By (Signature): '.$te/Time 05/02/11 E

__7 Sonic Delivery /

05/02/11 E..

Sonic Delivery 05/02/11 ETS 05/02/11 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 25 of 100

E--U--EI--

P*g 1\

Whole Effluent Toxicity Sample Receipt Log Date Time Received Received Sample Project Sample Sample name aud description State Comments received -ei-d by froM. teperature (C) nbe number numbr 05-02-I 1512 JSmner TVACouier 07,0.8 7068 110502.01 TVA- Seuoyah Nuclear Plant - 101 TN 05-02-Il 1512 J.Sumner TVACourier 1.2 7068 11050202 TVA - Sequoyah Nclea Plant-Itake TN Page 26 of 100

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Sonic. Delivery Facility Sampled: Sequoyah NP 28801 General Comments:

Ray Duncan:

NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell :

Sara Snyder:

Fax: 828-350-9368 Metals samples filtered and stored. Samples remained on By:Ra3 Dnan, Ben N~hla Sh~ ice through out sampling and transport to lab.

rC ec d /J1A~0/

a Fd Identificati on / rak/Comp. K'J> 'ollection Date/Time Container Flow Sample Description Number & MGD Rain Event?

r161ALaborato:ry Use~

Volume (Mark as Appropriate)

.1 Date Time By, I 7emp.

-TOX Comp I Relinquished By (Signature):

4 Date/Time 4.

Received B:

1 I

05/04/11 05/04/11 Er 1 I I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 27 of 100

-ErS PagelJ-3 Whole Effluent Toxicity Sample Receipt Log Date Tme Received Received Sample Project Sample Sample name and descrption State Comaseant received recelyed by from temperatue. (C) number number 05-04-11 0955 K. Keenan Fed - Ex 1.4 7076 11050401 Duke Energy Corporatlion - Belews Creek SS NC 05-04-11 0955 K. Keenan Fed - Ex 1.3 7077 110504.02 Bladenboro WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 1.2 7078 110504.03 Progress Energy Carolinas - Cape Fear S. E. NC 05-04-11 0955 K. Keenan Fed - Ex 1.2 7078 110504.04 Progress Energy Carolinas - Cape Fear S. E. - Upstream/Intake NC 05-04-11 0955 K. Keenan Fed - E. 1.0 7079 110504.05 City of Gastonia - Dallas WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 2.5 7080 110504.06 United Water - Enfield WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 2.5 7081 110504.07 United Water - Scotland Neck WWTP NC 05-04-I1 0955 K. Keenan Fed - Ex 1.0 7082 110504.08 Wilson WWTP NC 05-04-I1 0955 K. Keenan Fed - Ex 1.0 7083 110504.09 Elementis NC 05-04-1I 0955 K. Keenan Fed - Ex 1.0 7084 110504.10 United Water - Farmville WWTP NC 05-04-lI 0955 K. Keenan Fed - Ex 2.5 7085 110504.11 Progress Energy Carolinas - Mayo Electric Steam Plant NC 05-04-lI 0955 K. Keenan Fed - Es 1.4 7086 110504.12 Progress Energy Carolinas - Shearon Harris E&E Center NC 05-04-11 0955 K_ Keenan Fed - Ex 0.9 7087 110504.13 Progress Energy Carolinas - Shearon HarrisPlant NC 05-04-Il 0955 K. Keenan Fed - Ex 0.6 7088 110504.14 Carolina Beach WWTP NC 05-04-11 1000 K. Keenan UPS 0.5 7089 110504.15 Nficrobac Environmental - Rockingham WWTP NC 05-04-11 1000 K. Keenan UPS 0.5 7090 110504.16 Microbac Environmental - Roseboro WWTP NC 1 05-04-11 1000 K. Knenan UPS 0.5 7091 110504.17 Microbac Environmental - JP Stevens - Waan WWTP NC 05-04-11 1000 K. Keenan UPS 0.7 7092 110504.18 Morehead City WWTP NC 05 II 1000 K. Keenan UPS 06 7093 110504.19 vde County - Enmellhad WTP NC UPS shipping error.

05-04-11 1226 K. Keenan Dash Courier 05 7094 110504.20 OWASA - Mason Farms WWTP NC 05-04-11 1437 J, Sumner TVA Coarier 0.7/0.9 7068 110504.21 TVA - Sequovah Nuclear Plant - 101 TN 05-04-11 1437 J. Sumner TVA Courier 0.8 7068 110504.22 TVA - Sequoyah Nuclear Plant - Inlake TN SOP G4 - Exhibit 04.2, revision 06-29-09 Page 28 of 100

-I --.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): (Snc Delivery Facility Sampled: Sequoyah NP 28801 General Comments:

Ray Duncan:

NPDES Number: TN0026450 Phone: 828-350-9364 Ben Mitchell _

Sara Snyder:

Collected By: Ray Duncan, Ben Mitc , Sa n der * / Fax: 828-350-9368 Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab.

fteld Identification/ /Grab/Comp. Collection Date/Time Container Flow 0' j..

Sample Description Number & MGD Rain Event? Laboratory Use Volume Collected (Mark as Appropriate)

L i* o : , :i:: ¢ i :: ,!*:

Date Time - Yes if Yes, No Trace ETS Log -Arrival By Time Inches Number Temp.; an*e SSQN-101-TOX Comp eWl1 'l/t-r1*1 01160 2 (5gal) '0 °qiO* Qr-,lii -

SQN-INTAKE-T OX Comp os/05 .

-1i a3oOdI - 1 (2.5 gal) NA7"- 0 6; 010 ......

.. .t o- . : .. . - * ? . . a o at! 1.6.".

_______________Sample Custody - Fill In From Top Down kM Relinquished By (Signature): Date/Time Received By (Signature): - Date/Ti:

05/06/11 C/ Sonic Delivery / 05/06/1 1 4-Sonic De ery 05/06/11 ETS 05/06/1l t~ .S>.rn Er ___ __ ___ ___ ___ __ 555Er Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that .the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page 29 of 100

I'

/

ETS Page Whole Effluent Toxicity Sample Receipt Log Date Time Received Received Sample Project Sample Sample name and description State Comments received received b from temperatore CC) number number 05-06-It 0946 K. Keenan UPS 3.3 7089 110506.01 Microbac Environmental - Rockingham WWTP NC 05-06-11 0946 K. Keenan UPS 3.3 7090 110506.02 Microbaw Environmental - Roseboro WWTP NC 05-06-11 0946 K. Keenan UPS 3.3 7091 110506.03 Miccobaw.Environmental - Westoint - WagramWWTP NC 05-06-I I 1000 K. Keenan Fed - Ex 1.9 7076 110506.04 'Duke Energy Carolinas - Belews Creek Steam Station NC 05-06-11 1000 K. Keenan Fed - Ex 2.3 7077 110506.05 Bladenboro WWW'P NC 05-06-11 1000 K. Keenan Fed - Ex 2.5 7078 110506.06 Progress Energy Carolinas - Cape Fear S.E. NC 05-06-I1 1000 K. Keenan Fed - Ex 2.5 7078 110506.07 ProgressEner Carolinas -Cape Fear S.E. -UpsueamvIntake NC 05-06-11 1000 K. Keenan Fed - Ex 2.7 7079 110506.08 City ofGastonia -Dallas WWTP NC 05-06-11 1000 K. Keenan Fed - Ex 2.0 7080 110506.09 United Water - Enfield WWTP NC 05-06-11 1000 K. Keenan Fed- Ex 2.0 7081 110506.10 United Water - Scotland Neck WWTP NC 0i-06-lI 1000 K. Keenan Fed - Ex 0.8 7082 110506.11 Wilson WWTP NC 05-06-11 1000 K. Keenan Fed - E. 18 7083 110506.12 Elernentis NC 05-06-11 1000 K. Keenan Fed - E. 0.9 7084 110506.13 UnitedWater- FarmvilleWWTP NC 05-06-11 1232 K Keenan Dash Courier 1.3 7094 110506.14 !OWASA- Mason Farm-s WWTP NC 05-06-11 1515 J. Sumner TVA Courier 0.9,0.9,1.5 7068 110506.15 TVA -Se oyahNuclcar Plant- 101 TN 05-06-11 1515 J. Sumner TVA Courier 0.9 7068 110506.16 TVA - Sequoyah Nuclear Plant - Intake T _N SOP G4 - Exhibit G4.2, revision 06-29-09 Page 30 of 100

Page 1of 6 U .. ~EnvIronmentaITest~ing Solutions, Inc.

I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas i Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant County: Mf t100rC.'T0 Outfall: 101 I NPDES #:

Project #:

210206W1

-1o6&

0 UWO I Dilutionpreparation information:

Dilution prep (%)

Effluent volume (mL) 10.8 270 21.6 540 43.2 1080 86.4 2160 100 2500 Comments:

Diluent volume (mL) 2230 1960 1420 340 0 I Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:

I Organism age:

Date and times organisms 22.-T *,bt-%Otzi oO* L.. 1%,6 Randomizing template:

Incubator number and 4e-uL_,U,)

were born between: shelf location: X__._

I Organism source: ATW &*,Tt. A ?J0* .at-II Artemia CHM number:

Drying informationfor weight C4II H i determination:

I Transfer bowl information: pH = 1.-16 Temperature = .'&.L. °C S.U. Date / Time in oven:

Initial oven temperature:

Ot.o-t% 1410 60 *(-

Average transfer volume: Date / Time out of oven: -qtcx I 0. I' t.,Final' oven temperature:

Total drying time:

liD't_.

"i-IA0 Dailyfeeding andrenewal information:

Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 osl't-lk 4. tIAisL t4t5 ,OI /lOz.0tQ.. Os-O-II A*

__ os,-_ ocka %so t Av -41*- o-o.oI tiosoL.rL s.n--A 2 _ _m-, i3)qSaq. 2.% l0SO4, 2 -. *-.t4,-l 3 6S.01.*,1 06o% )d %Soo ___- II 0g. 2( II0So. Io.2 1 OS,-q

- is CS1 I1o 0 0100 kL .. ' al~l . tl11..

s a. is lIosof . 16 A ' S-I 6

os-CA II no&0 I - 11 wS6(,. is It 0o6. I( os-os-tl Lis A

--7 I is Controlinformation: Acceptance criteria Summary of test endpoints:

I %Mortality:

Average weight per initial larvae:

Average weight per surviving larvae:

07.

0.-110 0.1 1

< 20%

0.25mg/larvae

=

7-day LC50 NOEC LOEC

!7.

(

(OO.

IMI, I ChV IC 25 1 -2,'tAt, 1.

I__DOI__a Page 31 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I

Page 2 of 6 I EnvironmentafTestfng Soiutlons, Inc.

I I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date: OS-*- 1\

Survival and Growth Data Day CONTROL 10.8% 21.6%

A B C D E F G H I I J K L 0 10./()

t)_I o 1 0/0 t0 /0 /0I ) /0( 1 I 1 /0 (0D (0 10 It (0 10 0 (1 16 -Io I 2 I0 (b (0 to3 M t If 0t to 10 to Io 2 /1D I0U 3 11( 10 10 (0 /t/0 /0 /0 I 5 (C t to I*

to to 1. 10/ to*: to A - Pan weight (mg)

Tray color code:: L.'A't &Lt, I Analyst:

Da te :

Ai WC C A_. 04 ..itI B = Pan + Larvae weight (mg) 14.9(, 1 iIi 1S.6'

. t msi 1L-L.

o%5 ,t-b isit. 4 A Analyst: ffi.L Date: o0514-* 1, AvS.'J', t.,. to.I0 Z .11 Zit C = Larvae weight (mg) = B - A

., I iO 8 eA. 8.&q .'.8 (. .6o V, *.*bA .1'

! Hand calculated.

Analyst:

Weight per initial number of larvae (mg)

"l

= C / Initial number of larvae Hand calculated.

Analyst: _ , 0~ I. b' b o

- i - I- I - C- i - S- * -

Average weight per Percent reduction initial number of from control (%) 0.11 - lb. '".0 o. &S4 larvae (nmg).I I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

I Comments:

I I Page 32 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I

Page 3of 6 I

I FTS Envifrnmental Testing Solutions, Inc.

I I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date: CS. a".-Il Survival and Growth Data Day 43.2% 86.4% 100%

M N 0 P Q R S T U V W X 0 t0 Ito o o lo0/o o

'0- 10 to to1010 I to to 16 /0 to (0 /0 (0 1t /0 /0 ./0 I 2 16 10 10 to /10 to tot( (0 D0 I0 tQ 3 /0 I0 to t0o t( 10 /0 /0 I 10 (0 10 (0 (0 /0 10 to1 t10 to to0 t0 to to I 56 6

10 0

( 10 1O(O to to

/0 10 to t

/(Oto I 10 /0 o

to I(.

I (0 to (0

to

/0 to I 7 1b I to 10 I to (0 /0 tf 10 to I()

A Pan weight (mg)

! Tray color code::

Analyst:

Date:

kkmu.

05____A_.11 Livm St.L, M*.54 l.qi, 11513 1.1.4 l3-.? 5A) 15.Da*

IZr. '73.

.('n 13.44 I m.1*,1t I B = Pan + Larvae weight (mg)

Analyst:

Date:

M4.--..

VA zi4.U u(4UttML

~4.. e.. M Z--.(. ?..

l- l v.A.4 A010.0U11M

.O . ,,.9L -

C = Larvae weight (mg) = B - A I Hand calculated.

Analyst: 4.*

516 1-4C)S 9-A&44J~~ a-U 1. 1 .111. 1.

Weight per initial number of larvae (mg)

= C / Initial number of larvae .

Hand calculated. A. ¶ \0 % ,O.

Analyst: _ A-- V 1" 0, 0"" O" ('

Average weight per Percent reduction initial number of from control(%) 0.L2. -1.27. 3."' -- 2. 9,7 .1. -".1 "i, larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

Page 33 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I Page4of 6 I Environmental Testing Solutions, Inc.

I I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date: OS-C. \

I Day 100% Intake Survival and Growth Data Y Z AA BB 0

/L) (0 10. 1D 1

/0 /0 /0 1o I 2 3

/0 /0 to10

! 4 (0D 1o

10. 1oD 1 tO I) to I 5 6

Ic:1)10 tO

/0 10) IL 7

A = Pan weight (mg)

Tray color code:: LaAv SLa

! Analyst:

Date: t ItA P ok I B = Pan + Larvae weight (mg)

Analyst:

Date:

o5. 14-_

104 C = Larvae weight (mg) = B - A

! Hand calculated.

Analyst: I I,, Ql

" - t,.*

! Weight per initial number of la rvae (mg) .*

=C /Initial number of larvae Hand calculated.

Analyst:

! Average weight per initial number of Percent reduction from control (%)

-o.' -\,.

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.

Comments:

Page 34 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

..1- - . 1.ý TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 0 PIimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.

Prioijectnumber: 7068 Not forComplin-c Asssessment, Internal LaboratoryQC Cmontrll. p(%) Repliate loideet mbera Flaulmueaer tln-v A= P=

Pawnweiht(ng B=lPaoL rvae Laa ewelght(mg) WelghtlSar,,iv Meanw ightdIt Me Co.titeon..Ind. Weighti ulnfier

.tt Bluennsarviv.1 -wdea..wdghtlaiCtla Coaefiatd o Peoefrtrednitd=ntres I- weight uin (5aJ =A- B tsnsen( Srvbdtau S .. nmbeero W Zee "olase (rat) (%) iunberi larvae variation t4r- motrol (%)

larva (mrg) w .'wt(%) (rng) ,- snsbea A 10 10 14.85 2189 7.04 0.704 0.704 Control B 10 10 14.06 21.62 7.56 0.756 0770 6.6 0756 100.0 0.770 6.6 Not applicable C 10 10 14.34 22.33 7.99 0.799 0 0.799 D 10 10 13.19 21.38 8.19 0.819 0.819 E 10 10 13.36 21.85 8.49 0.849 0.849 10.8% F 10 10 13.89 22.78 8.89 0.889 7.0 0.889 G 10 10 13.81 . 22.79 8.98 0.898 0.898 H 10 10 12.55 20.23 7.68 0.768 0.768 1 1 10 9 14.01 20.60 6.59 0.732 0.659 21.6% 1 10 10 12.85 21.71 8.86 0.886 0.853 9.6 0.886 97.5 0M4 14.1 -8.4 K 10 10 13.91 23.04 9.13 0.913 0.913 L 10 10 14.49 23.28 8.79 0.879 0.879 M 10" 10 13.54 21.15 7.61 0.761 0.761 43.2% N 10 10 13.92 22.36 8.44 0.844 0825 5.2 0.844 100.0 0.825 S.2 -7.2 0 10 10 13.23 21.64 8.41 0.841_ 0.841 P 10 10 1t.74 20.27 8.53 0.853 0.853 Q 10 10 13.83 22.28 8.45 0.845 0.845 86.4% R 10 10 13.79 21.84 8.05 0.805 0791 6.8 0.805100.0 0.791 6. -2.

S 10 I0 15.00 22.16 7.16 0.716 0.716 T 10 10 14.76 22.73 7.97 0.797 0.797 1 U 10 10 13.63 21.64 8.01 0.801 0.801 100% V 10 10 13.44 20.40 6.96 0.696 0,790 8.8 0.696 1O0.0 0.790 8.8 -2.7 W 10 10 13.49 22.12 8.63 0.863 0.863 X 10 10 12.97 20.97 8.00 0.800 0.800 S 1010 14.35 23.28 8.93 0.893 0.893 00% Intake 110 60 13.38 21.32 7.94 0.794 0851 9.4 0.794 92.5 0.780 12.2 AA 10 10 14.45 22.19 7.74 0.774 0.774 BB 10 7 12.24 18.84 6.60 0.943 0.660 Outrfan 101: MSD = Minimum Significant Difference Dunnett's MSD value: 0.1196 PMSD = Percent Minimum Significant Difference PMSD: 15.5 PMSD is a measure of test precision. The PMSD is the minimutt percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake:

Dunnett's MSD value: 0.1052 Lower PMSD bound determined by USEPA (10th percentile) ý 12%.

PMSD: 13.7 Upper PMSD bound detenuined by USEPA (90th percentile) - 30%.

200 Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET tlterlaboratoty Variability Study (USEPA, 1a; USEPA, 2001 b).

USEPA. 2001a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-tct-m Chronic and Acute Whole Effluent Toxicity Test Methods, Volutmes I and 2-Appendix. EPA-82t-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

Page 35 of 100 Fi: sqn101_O50311datAldx Entered by: J. Sumner Reviewed by:

TVA / Sequoyah Nuclear Plant, Outfall 101

.Non-treated

" S 0

May 03-10, 2011 Statistical Analyses

,, Environmental Testing Solution s, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments.:

Conc-% 1 2 3 4 D-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000, Intake 0.8930 0.7940 0.7740 0.6600 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7695 1.0000 0.7695 0.7040 0.8190 6.623 4 0.8199 1.0000 10.8 0.8510 1.1059 0.8510 0.7680 0.8980 6.967 4 -1.643 2.410 0.1196 0.8199 1.0000 21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4 -1.305 2.410 0.1196 0.8199 1.0000 43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4 -1.114 2.410 0.1196 0.8199 1.0000 86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4 -0.428 2.410 0.1196 0.7908 0.9645 100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4 -0.413 2.410 0.1196 0.7900 0.9636 Intake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88409168 0.884 -1.217221232 1.2704004 Bartlett's Test indicates equal variances (p = 0.56) 3.9158771 15.0863171 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100 >100 1 0.11955186 0.15536303 0.00391794 0.00492163 0.566574454 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL(Exp) Skew IC05 >100 ICI0 >100 IC 15 >100 IC20 >100 lIC25 >100 1C40 >100 1C50 >100 File: sqnl0l_05031 ldata.xlsx Entered by: J. Sumner Page 36 of 100 Reviewed by:

I . ,

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 03-10, 2011 "TS Statistical Analyses 1 Environmental Testing Solutions, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 10 1 - Intake End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000 Intake 0.8930 0.7940 0.7740 0.6600 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.7695 1.0000 0.7695 0.7040 0.8190 6.623 4 10.8 0.8510 1.1059 0.8510 0.7680 0.8980 6.967 4 21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4 43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4 86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4 100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4 Intake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4 -0.199 1.943 0.1052 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98316872 0.749 -0.2349007 0.4704176 F-Test indicates equal variances (p = 0.33) 3.51607203 47.4683456 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.10523383 0.13675612 0.00023113 0.00586562 0.84920698 1,6 Treatments vs D-Control File: sqnl0l_050311 data.xlsx Entered by: J. Sumner Page 37 of 100 Reviewed by:_

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011

* ]Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 0

Daily Chemical Analyses Environmental Testing Solutions, Inc. Project number: 7068 Concentration Parameter Da 0 Day I Day 2 .Day 3 Day 4 1 Day 5 Dav 6 Initial Final Initial IFinal Initial IFinal Initial IFinal Initial IFinal IInitial IFinal Initial IFinal PH (SU) 7.84. 7.80 7.781 7.77 7.891 73781 7.811 T777 7.801 7.86 7.801 7T681 7.73 7.701 DO (mx/L) 7.4. 7.8 7.51 7.6 7.51 7.51 7.61 7.7 7.71 7.91 7.71 7.61 7.61 7.91 Conductivity (lunhos/cin) 333..323534 0 3 2 Control Aikalinity (mgIL CaCO 3) 5 95 Hardness (mgIL CaCO 3) 9 99

_________Temperature C 24.9 24.8248 4. 24.8 24.71 24.9 24.5'47 2. 24.71 24.71 24.8 .2.

PH (SU) 7.85 7 7.80 77 7.88 7.671 7.86 775 .82 78 7.93 7.64 7.80 76 DO ( /L) 7 7. 7 7.7 7. 7.5 7.61 8.2 7.8778 7.9 7.3 7.87.

10.8% Conductivity (uimhos/cm) 310 311 283 28.8 29.0 307 303

________Temperature ('C) 24.9 248 24.9 242 24.94 N24.6 25.0 247.48 24.5247 24.6 24.8 2.

oH (SU)78 7. 75M 7.79 77 7.87 7.66 7.85 776 .84 786.93 7.64 7.82 76 DO (gL) 7.7 7.7 7.7 7.5 7.9 7. 8.1 7 .8 78 9 8.1 7.3 7.91. 8.

21.6% Condiuctivity (Iunhos/cm) 292 291 272 275 275 284 28

________Temperature (C) 24.9 24.6 25.0 2. 24.9 244 25.0 24A7 24A8 244.471 2 24.8 24. 7 oH( SU) 777 77.77 7.70 7.86 7.661 7.84 777 .84 7.86 7.921 7.68 7.81 76 DO (mg/L) 7.7 7.7 7.7 74 7.8 75 878 7.8 7.9 8.1 7.6 7.88.

43.2% Conductivity (jumhos/cm) 253 257 241 242 24325 253

_______Temperature (1C) 25.0 24.5 25.0 24.6 24.9 246 25.01 24.6 24.8 245 24.7 24.8 248 24.6 PH (SU) 7.72 7.67 7.73 7.68 7.80 76 7.82 7.75 7.82 785.92 7.66 7.79 7.69 DO mI FL) 7.8 7.7 7.7 7.4 7.8 75 8.0 7.8 7.8 798.0 7.5 7.8 8.1 86.4% Conductivity (junhos/em) 179 183 185 187 11. 207 8

_______Temperature (T) 25.0 24.6 25.0 246 24.9 24.6 25.I 24.4 24.8 24.4 24.7 24.5 24.9 24.6 pH (SU) 7.711 7.681 7.72 7.67 7.761 7.661 7.81 7.74 7.811 7.851 7.90 7.64 7.79 7.71 DO (mg/L) .7.81 7T81 7.81 7.41 8.01 7.51 8.01 7. 7.81 8.01 8.1 7.41 7.7 7.8 Conductivity (pmhos/cm) 1571918.5 166 14417 100% Alkalinity (mgIL CaCO 3) 5 96 Hardness (mgIL CaCO 3) 5 37 Total Residual Chlorine (mg/L) <01<.0 Temperature ('C) 25.01 24.51 25.01 24.31 25.01 24.51 25.21 24.4 25.01 24.31 24.9 24.51 24.91 24.71 PH (SU) 7.691 7.681 7.721 7ý.64 7.751 7.651 7.81 7.73 -7.821 7.87 7.921 7.661 7.781 7.701 Do (mX/L) 7.81 7.81 7.81 7.3 8.01 7.51 8..0 7. 7.91 8.01 8.11 7.61 7.81 7.9 Conductivity (Puihos/cm) 15 5 5 59101812 100%o/intake Alkalinity (mgIL CaCO 3) 5 16 Hardness (mg/L CaCO3 ) 5 36 Total Residual Chlorine (gL :.0<.0'01 Tem rature CC Ei 25.01 24.51 25.11 24.3 24.91 24.51 24.9 24.4 24.71 24.3] 24.8[ 24.41 24.91024.6 File: sqn101 050311 chem.xls Page 38 of 100 Entered by: S. E ans Reviewed by:

Page 5 of 6 IldnIc I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101. Non-treated Daily Chemistry:

Date: os0*".%N\

I Analyst I

0 I Day 1

-4 I

Concentration IParameter I DO (miz/U -

Conductivity (umhons/cm)

CONTROL .1-Alkalinity Non-treated (ma CaCO,/L) 4-Hardness I (mg CaCO,/L) 1PH1 Temnerature (°C) 333.tIL .R -'tA I

(S.U.) c I 10.8% Conductivity (Trnehos/cm) 1963 _V. I I

______ Temperature (0CQ I__ I di ~ L'4 I 21.6%

pH (S.U.)

DO (mg/L)

Conductivity l

(19r IA 1 (jimhos/cm)

I Temperature (°C) oH (S.U.)

5 DO (melL) I __M___ _____ ___K.

I 43.2% Conductivity (g mos/cm)

TemI erature ("C)

S~

372. 1,+ s'. 2'A I 86.4%

pH (S.U.)

DO (ma/U Conductivity UI_

I-A

.39

-j.'j t.31 Temnerature (°C)

I ~ - - - -

MA j~lmlml*_.

pH (SU.) /4'-L1 4 -*~~-~-----I, - - - -

DO (mg/L)

Conductivity 161 (ghscm) j5~

Alkalinity 100% (mg CaCO 3 /L) _______

Hardness (mg CaCO 3/L) 55 TR chlorine (mg/L) 40.10 S

Temperature (°C)

S 1-S.0 oH (S.U.) 1,Ici (S.U.

pH______

DO (mLY_/*

Conductivity (gmhos/cm) /15 100% Alkalinity Intake (mg CaC0 3/L) 55 Hardness 5 (mg CaCO 3 /L) _,_______

TR chlorine (mglL) 0*1 Te....erat.... ( 0 -"I ~.LJII -1* LZI Teprtr ('Q I Initial 1ýiia Initial II Fi~nal I nitial Final Page 39 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09 tUAMAýNtftC

Page 6 of 6 I Envion m nta Tesing Slutlons, Inc.

Species: Pimephalespromelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. Non-treated Date: ln.I I Day 4 I 5 I Analyst Concen- Parameter tration I pH (S.U.) .Vol.

DO (ma/L)

L Conductivity (iimhos/cm)

j l4 *,.

-332, , Th1 *2-2.1 CONTROL Non-treated Alkalinity I (mg CaC0 3/L)

HardnessI (mg CaC0 3/L) 13A.

Temperature (MC)

S - - - - - -

pH (S.U.) ;t'.IN II . I1- 4 B1, II S-+- I "-4m- II .- 04 1I 7.0 11-DO (ma/L) 10.8% Conductivity (gmnhos/cm) AA 011' '3 03 Temperature (0C) -L-A. I 11A.Lý 'IM D S - S -L pH (S.U.) _1ý4 21.6%

DO (mg/L)

Conductivity (iimhos/cm)

[ZTL 71S.0 LLSJL 2

Temperature (°C) p p - - - -

DH (S.U.) II 1,4 I-~~~~~

I ~'1-4 II J.6 1-----*4 1 7-q.52_ II "1.A I 7Pd (l,".

DO (mR/L) 43.2% Conductivity (pýmhoslcm)

Temperature (0 m zj4 A'3--

4 DiH r (S.U.)

J i DO (ma/L) 10- 9 86.4% Conductivity

(ýmhos/cm) .1 k-Temperature ("M S - S pH (S.U.)

DO (mg/L) rd, D Conductivity (Amhos/cm) 100%

Alkalinity (mg CaC031L)

Hardness (mg CaC03/L)

TR Chlorine (mg(L) i Temperature ('C) U-4 . v I oH (S.U.)

DO (mgfL)o

--. 1 II',

~-~-*-----

mm 1 %,13 I"qi, 17tt 7 Conductivity I~

100% Alkalinity Intake "tq.

q 2. 19 Hardness-I

m a

TRchorine (g/L Temperature ('C) 24 Initial II Final Initial Final I Initial 11 Final I Initial )) Final l I Page 40 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

Page 1 of 7 I En Tom ItS ~ Iii -

I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)

I Species: Ceriodaphniadubia Client: Tennessee Valley Authority County: Hamilton i Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Outfall: 101 Project#: 1011A i Dilutionpreparationinformation:

Dilution prep (%) 10.8 21.6 43.2 86.4 100 Comments:

I Effluent volume (mL)

Diluent volume (mL)

Total volume (mL) 270 2230 2500 540 1960 2500 1080 1420 2500 2160 340 2500 2500 0

2500 ,

i Test organism source information-Organism age: < 24-hours old Test information:

Randomizing template color: 6.d%,]

Date and times organisms were born O$.G3P-%% 6"K T1 Mot Incubator number and shelf between: locaion Culture board: 2**\ location:

Replicate number: I1 2 3 4 5 6 7 9 10 bac Culture board cup number: I Ll IS I I\AI 11I l q YWT batch: o.l '-

Transfer vessel information: pH= 8.00 S.U. Temperature= 3A. °C Selenastrum batch: o4.1 I[

I Average transfer volume (mL): " S.*

I Day Daily renewal information:

Date Test initiation and feeding, renewal and feeding, or MHSW batch used Sample numbers used Outfall 101 Intake Analyst termination time I 0 ___ bk4 os-a-11A 'LOSca. 0 lkOo2.a2..

1 C6.L4- it tv12 OS.f5-itQ k 10o2.iL.

O 1 11oSoT..

2 _____it_ 12tO _05GLjLja la0sog42 Ir4SO4..2_

3 05-06-4 \%!as_ _ t,.(-k %*O' oI. t I %lS09. 2:L 5 c0oo.1%* I 21(at I 5-.*-t1 I 6 c&S.cA1 i -.L7 I it o I., is t* oe.0%. -1 I __A*_1b-1( I t-L-Lý I Control information:

_ Control-I Control-2 Acceptance criteria Summary of test endpoints:

% of Male Adults: 07- 07. !520% 7-day LC50 ICol.

! % Adults having 3ra Broods:

% Mortality:

Mean Offspring/Female:

tool, al 07.

100?.

_80%

20%

-.15.0 offspring/female NOEC LOEC ChV l0o*.

CO_______

It

%CV: 4.1.1. 1_ _.8_ <40.0/% IC 25 '7100".*

I Page 41 of 100 SOP ATI I - Exhibit AT 11.2, revision 04-01-09

a Page 2 of 7

-~ t,.i.o naaa ..O aSoran n I Species: Ceriodaphniadubia Client: TVA / Sequovah Nuclear Plant, Outfall 101 Date: .

CONTROL-I Survival andReproduction Data I Day 1 2 3 4 Replicate number 5 6 7 8 9 10 I Young produced 0 I Adult mortality. t r

~ ~

LI I L-C0.J~ 0L.

L.

.Adult mortality L 1I - "-- V.-I I 4 3Youngproduced Young produced

[.J 0 L_

0 C)I 00 Adult mortalityI 1-_l* *_ L 5 Young produced Adultmortality J -

I

.- K- * [ t I I __ i".

L ILII--

6 7

[ Young produced Adult mortality Young produced

")

v4 oI 1-4 11. l %S I

[ ____-_

v, tA I

L t*

I CI_0 C)

Ik-s v, V_L 0

Total young produced " U_. - ,.1 3 3 Ta k z. k ,.4 Final Adult Mortality X for 3rd Broods 3

t I \ - _ _

-A 54- X* . ' '

Mote:Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO - carry over (offspring carried over with adult durng transfer).

IConcentration:

% Mortality: I Mean Offspring/Female: 7.m I l CONC: 10.8% Survival and Reproduction Data Replicate number Day i T 2 3 4 5 6 7 1 9 1 10 Young produced 0. 0 Adult mortality_ t 1 1 k1 ,_ _

2 Young produced[I i[ 0] 0I 0[

Adult mortality _ j [* \.- I-I- lJll-3 4

[Young produced

[ mortality ngrdAdult

~

f.,___

~

I

~ t

~

~ 0 4 Young produced S A S '-A 5

I -Adultmortality I I- L I -- I I LtL ] .

Young produced I IS I lo -- t I Adl -otlt I- I - L - I -t-- tI--

Total young moproduced iA .30 6 .y 1- sa at ot witsau o n2r..s .

Final Adult Mortality _ ,k.. L .. ,_.

Notbe., Adult mortality (L -uvce D - ead), SB= splittbroodo(single brood splitbetween two daysi, Cv -ca over kous fnneamreoovery tna- -- " n--ras-er-LConcentration:

% Mortality: 07 I Mean Offspring/Female: 1 '30A

% Reduction from Control-I: 1- q' 07l Page 42 of 100 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09

Page 3 of 7 II tniirowi.otaimoiogs.osoni.iet Species: Ceriodaphniadubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date: CA-CM. 1A, CONC: 21.6% Survival and ReproductionData Replicate number Day 1 2 3 4 5 61 7 8 9 10 I Young produced r j 0 c . C

____ C.

_ _Adult mortality I. V I .- __ I _.- _ .

2 Young produced ( ) OF I Adult mortality L"-'] i-I *l -I V- ___-

3 produced~Young o 0 Adult mortality *_. 1 I U'.l 12A '4.

5 4 Young produced Adult mortality Young produced

~ ~ '--.-

%4 -

~

q.XIL I'

~ t~%41. '4

-I~

Adult mortality ' 1" I 6 [Young produced Qj 0 0 Adult mortality _ t U U k -. UJ_. _

7 Young produced [= -1% -I ___ __

Total young produced ,a. \ : _ .' "L

3( 3I W. T-- 31 I-I-Final Adult Mortality %' \- I \.- - I \_ %-

Note: Adult mortality (L - live, 0 = dead), SB = split brood (single brood split between two days), CO -ca over (offisprisncarned over with adult during transfer)

Concentration:

% Mortality: 07.

Mean Offspring/Female: I ,°i I

% Reduction from Control-I:1 CONC: 43.2% Survival and ReproductionData Replicate number Day 1 2 3 7 10 I

4 5 6 8 9 1 onpoueAdult mortality TIYon prdue

(.

5

ý) I \_ k 5

Fc

_ L_* L *..

2 Adult mortality Young produced Q Q I' V__ I C1. 1. %, .W C[

3 Young produced C> IC)o 0 I_0]oJ 0 Adult mortality I _.

I 3 Youngproduced Adult mortality ta

ý..

U_ 1 J[

j 0 L.i i

]...*_ ..-

t Ii 4 Adult mortality.

Young produced t 2

E 0)4n

-l

__ Adult mortality L_ 01 1- L_

04 7 Young produ-ce-dTti I ISTjk.-ct-r__ t4% %I IS Total young produced * . cI as is q 2 .-

Final Adult Mortality I .... i.. I

! .. ' _ '_ '_ .. I .

Note: Adult mortality (L = live, D - dead), SB - split brood (single brood split between two days), CO = carn over (offtspring carried over with adult during transfer).

Concentration:

%Mortality: 0:7.

Mean Offspring/Female: 31h.

% Reduction from Control- 1: - ISJ. ,

I Page 43 of 100 SOP AT II - Exhibit ATI11.2, revision 04-01-09

Page 4 of 7 n 5f Species: Cerlodaphniadubia Client: TVA / Seauoyah Nuclear Plant, Outfall 101 Date: OS-0"3-tk CONC: 86.4% Surv ival and Reproduction Data I

Day _ 2 3 4 5 6 7 8 9 10 1 SAdult Young mortality produced t*0 0%-'-0 00d.* *,1O I *-

Adult mortality ' t-- __-

3 Young produced 0 4 Adult mty Young produced~ _-*s o - Q-SI_~T.~ - -- 1- C.

_ Adult mortality .. _ _ _ L. U ' .. .. _ .

1l I A I3 I 5 Young produced 7 7 Young Adultmortality onproduced prdu l*

16

-*j L l

%115

t k.

"L.-O*-

-Lo L-

".'7-.1

-L U-J--

\

O L.IL IS lot otL Total young produced S C 56 1 56 asO s Final MortalityAdult I \._ - % ] jM .-

Note: Adult mortality (L = live, D = dead), SB = split broad (single brood split between two days), CO = cart, over (oftsring carried over with adult during tramrser).

Concentration:

I %Mortality:

Mean Offspring/Female:

% Reduction from Control-I: -

0 7, 34~A r'1.S7, CONC: 100% Survival and Reproduction Data Replicate number Day 2 3 4 5 6 7 8 9 10 I Young produced Adult mortality 0

] - (

C

) C I0 I° 2Young produced cQj C) r)I 0 0 0 0 0.......

Adult mortality [L-..L ,-_-.1 .-1.. l .I .. . .- 1.*

[

3 Young produced Adult mortality I ) n =

I 4 Young produced Adult mortality [_ J VI_ LtI--_'.--I_ ___

5

__ Adult mortality Young produced jA

%--I--+~-~

I -L

L 2-I+/- \

- :J"

- ,". ý1 1 IO 6

IAdult Young produced mortality li r)f o o__L..J__

I, 7 Young produced i Lj ~ 1,t Total young produced Final Adult Mortality j.

j. t. . -

as*

S.

3 (6 3 c....

6 5*

te:l¢ M*IUltmlortltlly Ik. ý Ilive,D = dead), So - splitl brood (inglt*e broodI split between two da~ys), CO.= C4r over (offlOnca**ei overwits**adlt dnuorg trantser).

Concentration:

% Mortality: 07.

Mean Offspring/Female: 3I .4

% Reduction from Control- 1: - - ,, 7, I Page 44 of 100 SOP ATI 1 - Exhibit ATI 1.2, revision 04-01-09

Page 5 of 7 I

T ___

,.* E*V&luenmmnimtlTnlagsauo'utihint.l Species: Ceriodaohniadubia Client: TVA / Seq uoyah Nuclear Plant, Outfall 101 Date: oS-qf*-%X CONTROL-2 Survival and ReproductionData Replicate number Day 1 2 3 4 5 1 6 -7 8 9 10 1 Young produced Adult mortality__

k-

- - 0

\- . - I__ ,-I--

Adult mortality t- 11i . 1

_ Adult mortality U.. . U" .

'-.,U- U U U-4 Yongduied I O %

Adult mortali

. 7I 4 _ U __ S 7 6 Young Adult mortality Young produced 1

.- 7. ] 1t 1 1 5o

.'-.K t* C) lI1~ I--

0~~

u-5 produced k * -1 I

____ * * ' b k 7Young produced k~.O.C S

T ota l yo ung pr od uce d 2 0!t 7. o WA : o " .A 1 1.S 3 ". *3 k 7- Ij Final Adult Mortality = C-- C t.- %. \...

X for 3'd Broods Note: Adult mortality (L = live. D = dead), SB

"..~...* . ". I- - I >-

split brood (single brood split between two days), CO= carry over (offspring carried over with adult during transfer).

IConcentration:

%Mortalit: 07.

Mean Offspring/Female: 0 CONC: 100% Intake Survival andReproduction Data Replicate number I Day I Young produced Adult mortality 1

C) 2 3 0

V-[

4[

5 6 U

7 U J 8

U 9

I 10 I 2 Young produced Adult mortality 101 0 DL L IU L L J LU ' 0 i

'- IO 3

___IAdult Young produced mortalityU Young produced _

-U

"'. I {O U

%4 IO jU Adult mortality U b-..---K-i-I'I U I \- [

5 Young produced t lo I'L I4 cLVL)lj 1,3 __

Adult mortality I ýX %'L ...... L. L L .

6 Young produced - _ _ 6 Adult mortalityI L1 T1. .__-I L- L.. ___ _-_- _

tot 7 Young produced Total youngproduced .

T1 X k

s

10 s j

. '"(

I *-i I I&

2.. 1 2b,=

21 3

1 _

3L,,

Final Adult Mortality . i. .- ' .

le: MtuUtmortaltt) k, = ive, D/ - deaul, SB - spllt brord]l kSlinle brood spill betweenl two dasy), COU = - overl *.to p nlll c;tllt olverI ttU U =Itg twls-).

u¢ Concentration: .

% Mortality: O*

Mean Offspring/Female: 35.

%Reduction from Control-2: -- o .s7.

SOP ATI 1- Exhibit AT 11.2, revision 04-01-09 Page 45 of 100

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011 ETS E vo ne W Control-i e t fgoh f h

~I Verification of Ceriodaphnia Reproduction Totals 86.4%

-. -- p-Day Replicate number Total Day Renlie*q te number Total I L 3 4 5 6 71 8 1 T9 10 Ta Da -y-r number 3 14 1 5 I 6 7 1 8 1 9 1 10 i 0 0 0 0 0 0 0 0 L 0 -0 0 0 0 0 1 0 1 0 10 0 0 0 0 2 0 0 0 0 0 0 0 J0 0 0 2 10 10 0 10 10 10 10 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 10 10 10 0 !0 0 10 0 4 4 4 4 3 4 4 5 4 4 4 40 4 5 4 5 5 4 4 6 4 6 5 48 5 10 11 11 12 11 12 12 10 12 12 113 5 13 12 12 14 12 11 I1 12 12 12 121 6 0 0 0 0 0 0 0 0 0 0 0 7 16 19 16 16 20 22 19 19 15 18 180 7

Total 14 28 14 28 16 31 15 31 15 31 15 31 14 30 15 29 13 29 13 29 144 297 To,'I 34 35 33 135 136 137 136 135 133 15 .4 49A 10.8% 100%

DyReplicate number Total ]~ * -.

Day U8 'A !t!N*I Total I 0 0 _0 6 7 08 9 10 I 2 13 4 6 7 8 9 1 0 a1y 0 D1 0 o 1 0 0 0 0 0 0 0 0 0 0 0 00 2

3 0

0 0

.0 0

0 0 0 00 2 0 0 10 10 10 0 0 1 0 3 0 0 0 10 0 0 0 0 0 0 0 4 3 5 5 4 4 5 4 4 4 6 44 4 5 5 6 5 5 4 6 6 4 5 51 5 13 10 13 12 10 12 12 10 11 11 114 5 14 12 1313 12 14 12 12 12 12 126 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 3 3 16 1 4 14 1 515 '5 1251 7 1 6 1 7 19 19 17 19 18 20 18 18 20 19 187 Total 33 1,30 34 32 27 33 30 28 30 32 309 Total 38 36 36 37 35 38 36 36 36 36 34 21.6% Control-2 Day 1 2 3 4 Replicate number 5 6 7 1 8 1 9 10to Total Day ~Replicate numberToa 1 2 3 0 4 5 6 07 8 9 10 T 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0- 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 3 4 4 4 4 4 40 4 4 3 5 3 2 30 32 3 4 4 36 5 12 lO 12 13 12 12 12 12 11 11 117 2 12 10 12 11 I0It 11 12 12 10 112 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 17 15 19 14 16 15 5 16 18 162 7 3 13 17 16 15 13 15 17 115 15 1 149 Total 129 26 134 130 29 27 30 32 31 29 1 297 1 Total 33 32 31 36 29 32 31 31 31 33 319 43.2% 100% Intake Dy 1 2 Replicate number 8 0 Total Day ~ Replicate numberToa Day 1 2 3 4 5 6 7 8 9 10 I_[ 24 3 4 5 16 7 8 49 14 40 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 L0 0 0 0 0 4 6 4 5 4 4 4 3 4 4 4 42 4 6 4 5 4 5 5 4 4 4 4 45 5 11 12 14 11 12 12 13 13 13 12 .123 5 13 12 14 It 12 12 10 13 13 If 121 0 r? 0 0 0 0 0 0_1 0 1 6 0 0 0 0 0 0 0 0 0 0 0 7 19 16 15 15 19 20 19 17 15 18 173 7 18 21 16 20 20 18 18 19 21 21 192 Total 36 32 34 30 35 36 35 34 32 34 338 Total 37 37 35 35 37 35 32 36 38 36 358 File: sqnl0l_050311data.xlsx Page 46 of 100 Entered by: J. Surmner Reviewed by: d

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test 0 EPA-821-R-02-013, Method 1002.0 Quality Control

3 Environmental Testing Solutions. Inc. Verification of Data Entry, Calculations, and Statistical Analyses Project number: 7068 Concentration Replicate number Survival Average reproduction Coemcient of Percent reduction from 3 4 5 6 7 8 9 10 1%) (offspring/female) viation (%) control (%)

(%) 1 2 Control - 1 28 28 31 31 31 31 30 29 29 29 100 29.7 4.2 Not applicable 10.8% 33 30 34 32 27 33 30 28 30 32 100 30.9 7.4 -4.0 21.6% 33 32 31 36 29 32 31 31 31 33 100 31.9 5.8 -7.4 43.2% 36 32 34 30 35 36 35 34 32 34 100 33.8 5.7 -13.8 86A% 34 35 33 35 36 37 36 35 33 35 100 34.9 3.7 -17.5 100% 38 36 36 37 35 38 36 36 36 36 100 36.4 2.7 -22.6 Control - 2 29 26 34 30 29 27 30 32 31 29 100 29.7 7.8 Not applicable 100% Intake 37 37 35 35 37 35 32 36 38 36 100 35.8 4.7 -20.5 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 1.698 PMSD= Percent Minimum Significant Difference PMSD: 5.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake:

Dunnett's MSD value: 1.569 Lower PMSD bound determined by USEPA (10th percentile) = 13%.

PMSD: 5.3 Upper PMSD bound determined by USEPA (90t percentile) = 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-M1-005, US Environmental Protection Agency, Cincinnati, OH.

File: sqn101_050311 data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Page 47 of 100 Spreadsheet entered by: J. Sumrer Reviewed by: ILA

I. . . 4 .. i , ,

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 0 *,

iý EvTSo Environmental Testing Solutions, Inc.

Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/3/2011 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments:

Cone-% 1 2 3 4 5 6 7 8 9 10 Control- 1 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 Intake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean Control-1 29.700 1.0000 29.700 28.000 31.000 4.214 10

32.933 1.0000 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10 10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10 -1.616 2.287 1.698 32.933 1.0000 21.6 31.900 1.0741 31.900 29.000 36.000 5.809 10 -2.963 2.287 1.698 32.933 1.0000 43.2 33.800 1.1380 33.800 30.000 36.000 5.717 10 -5.523 2.287 1.698 32.933 1.0000 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10 -7.005 2.287 1L698 32.933 1.0000 100 36.400 1.2256 36.400 35.000 38.000 2.654 10 -9.025 2.287 1.698 32.933 L.0000 Intake 35.800 1.2054 35.800 32.000 38.000 4.711 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.829582155 1.035 -0.138685175 0.300894097 Bartlett's Test indicates equal variances (p = 0.13) 8.592660904 15.08631706 The control means are not significantly different (p = 1.00) 0 2.100923666 Hypothesis Test (I-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 I 1.697549464 0.057156548 64.58666667 2.755555556 1,9E-12 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples)

Point % SD 95% CL Skew IC05 >100 ICI0 >100 IC15 >100 IC20 >100 lIC25 >1001 IC40 >100 IC50 >100 File: sqn ! 01 05031 ldataxlsx Page 48 of 100 Entered by: J. Sumner Reviewed by:

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 03-10, 2011 "EnI 0

Statistical Analyses

'* Environmental Testing Solutions Inc.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/3/2011 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Diseharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments:

Conc-% 1 2 3 4 5 6 7 8 9 10 Control-I 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 Intake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 29.700 1.0000 29.700 28.000 31.000 4.214 10 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10

10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10 21.6 31.900 1.0741 31.900 29.000 36.000 5.809 10 43.2 33.800 1.1380 33.800 30.000 36.000 5.717 10 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10 100 36.400 1.2256 36.400 35.000 38.000 2.654 10 Intake 35.800 1.2054 35.800 32.000 38-000 4.711 10 -6,741 1.734 1.569 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.949571669 0.868 -0.13778615 0.558140392 F-Test indicates equal variances (p = 0.36) 1.87890625 6.54108572 The control means are not significantly different (p = 1.00) 0 2.100923666 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.569196635 0.052834904 186.05 4.094444444 2.6E-06 1, 18 Treatments vs Control-2 File: sqnl0l_05031 ldata.xlsx Page 49 of 100 Entered by: J. Sumner Reviewed by:

I.

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 o

Daily Chemical Analyses OEnvironmental Testing Solutions, Inc. Project number: 7068 m

Concentration lParameter 6 I DH (SUl DO ImpJLI Conductivity (umhos/cm) I Control zz91 Alkalinity (mg/L CaCO3 )

Hardness (mg/L CaCO3 )

A. ^1 *. m

______Temperature CC) 2449 4 247 24.9 24 .291 24.91 24.91 25.0 249. 29 24.81 25.2 24.91 25.21 PH (SU 7.855 77 7.80 790 .881 7.851 7.86[ 7.86 7.82 771.93 71 7 801 7800 DO (umgL) 7.88 7.6 775 7.51 7.71 8.2 78.778 7.91 7.8 7.87 18% Conductivity (Lwnhos/cmn) 310) 311 283 288 29) 307 303 oH24,9 784 7717 9 8 8 8 8 8 9 9 8 8 7

________Ternperature ('C) 1 25.0 2 25.0 24.9 29 25.0 250. 4820 2520 24.9 2515. 25.0 pmH(U) 777 7764 7.779 .9 7.86 7.86 7,845 8 7.84 70 7.9238 7.8127 2.% DO (mg/L) 7.7 7.6 7.7 7.6 7.8 7.8 801 7.8 7.8 8.0 8.1 7.8 7.8 7.7 42% Conucntivit pmnlos/eni 252 257 272 241z -245 254 2853

______Temperature (*C) 25.0 2519 25.0 24.9 25.0 2510 25.01 24.81 25.0 2502 24.9 25.1 25.0 25.1 pH (U) 7.72 7.74 7.73 7.86 7.801 7.86 7824 7.861 7.82 7.93 7.921 7.831 7.79 7.78 86.4% DO nnaL) 787 7.6 7.7 7.6 7.8 7.8 8.0 778 7.8 8.0 801 778 7.8 7.9 Conductivity (1umhos/cm) 174 ý 183 218 18748 207 189 Temperature ("C) 25.0 24.8 25.0 24.9 25.0 24.9 25.1 24.9 25.0 25.0 24.9 24.9 25.10 225.2.

PH (SU) 7.72 7.72 7.72 7.85 7.76 7.86 7.81 7.86 7.81 7.93 7.90 7.83 7.79 7.77 8.% DO (mpIL) 7.8 7.5 7.8 7.61 78. 7.8 8.0 7.7 7.8 8.0 8.0 7.8 7.7 8.0 Conductivity bimhos/cm) 1579 189 158 1587 166 274 1 171 Temperature ("C) 25.0 24.81 25.0 24.8 25.01 25.21 25.2 24.9 25.0 24.9 24.9 24.9 25.1 25.2 9H(U) 7.69, 7.721 7.72 7.83 7.751 7.841 7.811 7.85 7.82. 7.91 7.92 7.83 7.789.7 DO (mgL) 7.81 7.61 7.8 7.7 8.01 7.81 8.01 7.8 7.91 8.1 8.11 7.7 7.87.

Conductivity (jnmhoslcm) 155 15 15715 17017 172 100%Itk Alkalinity (mig/L CaCO 3) 55616 Hardness (mg/L CaCO 3) 5 36 Total Residual Chlorine (mg/L) <0.1 00 <0.0

_______Temperature (*C) 25.01 24.91 24.91 24.81 24.91 25.21 24.91 24.8 24.91 24.91 24.91 24.91 25.1 25.2 File: sqn101_05031 lchem.xls Page 50 of 100 Entered by: S. Evans Reviewed by:

Page 6 of 7 EM,.m ~.Th~gS ~

Species: Ceriodaphniadubia Date: OS-f6-'

Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry:

Day 12 Concentratio n Parame CONTROL Alkalinity (mg CaCO3/L)

Hardness (me CaCo 3 /1,)

__________Temperature ('C) i l oH (S.U.) 310 .1~~~l 14411 r~.'A9')

V l~Oh~

LL!IL.L9 J I

10.8%

DO (ma/L)

Conductivity (jimhos/cm)

I 1 9

Temperature (C)

. 9-U.l-0 UI 0I.S, , I -

pH (S.U.)

I 21.6%

DO (mg/L)

Conductivity A~

(iimhos/cm)T Temperature (C) I ."dt I

'. . . D. -

oH (S.U.) '~JIL1L~L' I "J~-~ !I2rAO DO (mr/L) -Ii-I '

43.2% Conductivity (Prnhos/cm)

Temperature (°C) i ~

VSI IEI1 1Ls.I I oH (S.U.) II 44 *-+. ++, ::. !

86.4%

DO (mp/L)

Conductivity

1-73Z ME (jimhos/cm),

Temperature (°C) u Ii ii 9 - 'I ~

r pH (SU.) "-4',-1 II *1.

l.===..,.--

DO (mp./L) "-0 II Conductivity

..pmhos/cmn) 7-"

Alkalinity 100% (mngCa(-O 3/L) 5A Hardness (mg CaCh3lo (g/L)

TR chlorine (mgfL) <Q, 11D 40.10 Temperature (°C) "L.o0 II .L4.f I .S l ".6*.1I I l* '

p oH (S.U.) "J.dfII 1~5I DO (mg/L)

Conductivity (Mumhos/cm) 100% Alkalinity Intake (mg CaCO3/L)

Hardness (mg CaCOt3/L)

TR chlorine (mag/L)

- " i.

(0,10

=" - (0.

Temoerature (°C) 7S 0 II q .j .

-I I

Initial Initial Final Initial Final Page 51 of 100 SOP AT 11 - Exhibit AT 11.2, revision 04-01-09

Page 7 of 7 E ~to .,., 1~U ;S ~

Species: Ceriodaphniadubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date: O'S-*-b-\

I Dav I

Day v- ---

5 Analyst I. 4 I Concen- Parameter trtio ___________

PH (S.U.)

DO (mg/I)

Conductivity (gmhos/crn)

CONTROL Alkalinity (mg CaCO3IL)

Hardness (Mg CaCO3L)

Temperature (MC) ~.

pH (S.U.)

DO (mg/L) 10.8% Conductivity (grmhos/cm) 10 IVA -4_.97. e V 8 1 Temperature (°C) II ~~ I-~ "0*,

"-s*

L t .'.L ! .'* II" .* I"otII U W ~. -- - - - .1 PH (S.U.) :7r.Q9 1I ,g k1 ., 11Wq! I 120 117.ARlI 1I 7,Al11Z J2.9-S 1* - - U-DO (m./L) .1 LMLThJ 179 21.6% Conductivity (4fmffiS/CM) IRS+ 155 Tempue (c) pH (S.u,) - iL9~4.1 ~.84

- II L -ts. C)

"IPj I i

DO (me/L) 7i.153 43.2% Conductivity

_(grhos/cm) i Temperature MC)

.. . a pH (S.U.)

DO (m/L) , , -Le I -I1 86.4% Conductivity (jimhos/cm)

Temperature ('Q PH (S.U.) 1 II -

4 I I'M . I 1 - 9';i II I. -

II I-DO (mn-/L) * ~.- -AA -- L-1 Conductivity (Amhos/cm)

Alkalinity 100% (mg CaCO 3/L)

Hardness (mg CaCO3/L)

TR Chlorine (rag/L) A 0.1b Temperature (MC)

S S - - -

pH (S.U.) I ".l'6 it1 -. 4 I 4,t-a16 1 DO (mE/L)

Conductivity 100% Alkalinity Intake Hardness ;C .'1 TR chlorine (mg/i.)

I S Teemperature (M) U11 111 .7-4. -V 1 Initial-i -Final I 7nitial I Final 1 VC-11 I Initial

'4

ýFinal 1 1S. 1 I initial jr 11 1b, I-Final I

Page 52 oflO00 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09

0 Page 1 of6 Enviromental Testi ons, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: Tennessee Valley Authority County: . tA ILoTo4 Facility: Sea uoyah Nuclear Plant Outfall: 101 NPDES #: eOý ý TM Otc AS Project #: ___ ___

Dilution preparationinformation: Comments:

Dilution prep (%) 10.8 21.6 43.2 86.4 100 Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2230 1960 1420 340 0 Interferences.

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:

Organism age: 24 -IS

, 00...* OUS. Randomizing template: &

Date and times organisms OS1.-t4 t. Q Incubator number and were born between: shelf location:

Organism source: , ' G<-Cn.oI Artemia CHM number: ,4. S 'IA Drying informationfor weight determination:

Transfer bowl information: pH = -1. 16 S.U. Date / Time in oven: 0S.O-%0.., 110 Temperature, "1.'L "C

' Initial oven temperature: !,, .

Average transfer volume: Date / Time out of oven: os-P-AI i41 0- In .,4 Final oven temperature: ,

Total drying time: IA-.KOlls Dailyfeeding andrenewal information:

Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 g tJ .\l-loOu * <

1 S z.ol ,,Ho<aza m. os*-n-11 l oS'ut*- IL- o4co -

.k 1*>

tSA 4- 1 tt.0a,o0 %ose..

n- as ý-,it A 2 I 0O.* IS00 - t3op If iIoScA.21 I IoS'.4... oS.j'.-tlB6 5oa.-d CAOo I3IOfO. is t15o0, it. e&0-og it 6

- 4 it0SoL.. LS tiOSoL. (( 6e.a-l%

7V OS-'%JO. .

16g2_1 4I I*

Control information: Acceptance criteria Summary of test endpoints:

% Mortality: 67-- 20% 7-day LC50 t17.

Average weight per initial larvae: 0.__1& w, NOEC Average weight per surviving larvae: 0.L1 _ > 0.25mg/larvae LOEC >1?7.

ChV >t1617.

IC25 >tool.

Page 53 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

FTS Page 2 of 6 1 SEnvironmental Testing Solutions, Inc.

I Species: Pimephalespromelas I Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date:

Survival and Growth Data neJa.L\,i I Day 0

A to CONTROL B

10 C

10 D E F 10.8%

G H I J 21.6%

K L 10 1 to.[0 10 10 /0 to to I I 0 to to to to to I0 to /0 0 I0

/0 to 2 to0 I 1 0 to - 1to to /01 0 toI0 to to to 3 to to 10 loC'1Itb 1( 01t tO I(Q to0 t 4 It) 0 /( I0() 10 to to /0 I0 to /0 !(0 5 11D 10 10 1bU 0 1U t) /0 10 0 to t0O1 6to to. to /0 16 10 10 lo / to /0 10

/0 11,/

0 !0 /D 10 0 to to /0I I A =Pon weight (mg)

Tray-color-cde::

Analyst:,,'r, _ :J0 " I3.14( %?,.4:-R I*. .OA it.3, ts*.4~ 14,9* 14,64 14-.06 *. ts- .t Date: 0 C01104 0 /

8 = Pan + Larvae weight (mg)

Analyst: _ ~ t I Date: OSg. 14. it{ ,, m ~tz-( a4 '- ý .34 (-qtWKU C = Larvae weight (mg) = B- A HandTcalulated.y clor ode:: .0c & .,

Analyst: ,, _ _ _ . . 1 3 DIe Weight per initial number of larvae (mg)

= C / Initial number of larvae I Hand calculated.

Analyst: L V 0.

"P I

Average weight per Percent reduction a a b a initial number of from control (%) j

larvae (rag)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, I Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

I I

I Page 54 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I

FTS Page 3 of 6 I Snvironmental Testingsolutions Inc.

Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101, UV-treated Date: c&.%k Survival and Growth Data Day 43.2% 86.4% 100%

M N 0 P Q R S T U V W X 0 lb 1IC Q 1 0I ) 10 10 0 iQ 'O /0 tO 1 /0toD0 16 0 10 0 (0 0 /0 /0 /0 2 (o 1010 10(0 o t (t 0o to /o to 10 to 3 ID 10 10 1( 10 (0 /0 t /0 o /0o, /0 4

,4 10 t o (0 1() (0 (0 to 10 10 0 /o to 5 (C t L1 0 IIo 10 10 '1 ct 6 (01 0(0

)0 t at 0 /O O '/0 7 ,

IO() (() A () 1 `1, 1U O IL) /6 '

e (mg)

A = Pan weight Tray color code:: Mmw-rc Analyst: MItA, i0 4A4L 11A1,, 14.?I . 15,-n I4,U, (4-.L4 ttS it.m- 1I, A-.S Date: 0o. o4,.f B = Pan + Larvae weight (rag)

Analyst: _ _0t _ (i 0 . .41 1 ..

Date: os.1*.!t MS5. Mt,.01*' LL.L MZo MA ; t,.L' 2..L t. 3 L..o$ t-.(41 . q .5 C = Larvae weight (mug) = B - A Hand calculated. t, 8'41 10.1tj 2.S i.-I. ,1- "o*' f.l.1 I. QL. l1 (..(.b Analyst:

Weight per initial number of larvae (rag)

= C / Initial number of larvae Hand calculated.A . 0(* AN~

Ana-yst: - I -

Average weight per Percent reduction initial number of from control (%) OAS& - o.&l 0:.1"a q.0. Oa&0-larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

Page 55 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

Page 4 of 6 Envrnmena Teta luttons.Inc.

Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, U-V-treated Date: n Survival and Growth Data Day 100% Intake Y Z AA B 0

I D10 2

3 4

5 6 /0 t lo10 7

A = Pan weight (mg)

Tray color code:: C Analyst: Vk)4 Date: o ct -o_-i B = Pan + Larvae weight (mg)

Analyst:

Date: OG-14. n C = Larvae weight (mg) = B - A Hand calculated.

Analyst: .__. a*.....

-ogl A - -,.611 Weight per initial number of I

= C / Initial number of larvae Hand calculated.

Analyst: i l

Average weight per [ Percent reduction

a a initial number of from control (%)

larvae (m2) I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.

Comments: i Comments:

Page 56 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

TVA / Sequoyah Nuclear Plant, Outfall 101 U-V-treated May 03-10,2011 Pimephalespromelas Chronic Whole Effluent Toxicity Test "ETS EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Project number: 7068 Not for Compliance Assessment, Internal L or, OC Cmtmfle (%) Replicate lnbtoIm obfedFinal .. ber duov. A- Pweigitd(ewg B = Pa +Lae La-wewe t (mg) W41ttlSo-lring Monwa-ightI Caffiontjrbftio WeAklgn/ .ns1b. Meancrr' Mm.rdettlt/.I C r Poserelnofcotur.f-rn N

houe. weoight(m - A-B Sennidegftmberd 8-beheoderre.(ea (Mov."ss dtune(.j- (%1) mWereheeen necrntin. (.t.

-- etdA (%)

Ieave. (acw

) l * * ,6,,,

A 10 10 14.07 21,21 7.14 0.714 0.714 B 10 10 13.19 22.27 9.08 0.908 0*908 Con trl C 10 10 12.59 21.12 8.53 0.853 0.831 9.9 0.853 100,0 0.831 9.9 Notapplieable

_D 10 10 13.38 21.87 8.49 0.849 0.849 E 10 10 15.09 24.10 9.01 0.901 0.901 1 t0 10 13.35 21.48 8.13 0.813 0.813 10.8% G 10 10 13.24 21.91 8.67 0.867 0.867 4.4 0.813 H 10 10 14.87 23.72 8.85 0.885 0.8s5 1 10 10 14.68 23.64 8.96 0.896 0.896 21.6% J 10 10 14.06 21.99 7.93 0.793 0824 6.3 0.793 1000 0.824 63 0.8 K 10 10 13.16 20.95 7.79 0.779 0.779 L 10 10 13.14 21.43 8.29 0.829 0.829 M 10 10 14.76 23.55 8.79 0.879 0.879 N 10 10 14.42 22.94 8.52 0.852 0.852 0 10 10 13.76 22.23 8.47 0.847 0.847 P 10 9 14.39 22.12 7.73 0.859 0.773 Q I0 10 13.83 22.33 8.50 0.850 0.850 86.4% R 10 9 15.23 22.93 7.70 0.856 0.819 4.9 0370 97.5 0.798 4.6 4.0 S to 10 14.26 22.00 7.74 0.774 0.774 T 10 10 14.64 22.61 7.97 0797 0.797 U 10 10 12.55 20.71 8.16 0.816 0.816

____ ___0_ 10 11.96 (9.98 8.02 0.8020.2 100% W 10 14.80 lO I0 14.80 1.40 21.40 6.0 6150 0.80 0660 0.803 13.9 0.802 0.660 97.8 0.780 10.4 6.2 X 10 9 14.85 23.25 8.40 0.933 0.840 Y 10 10 13.26 22.74 9.48 0.948 0.948 Z___ _____0 _ lO0 13184 22.67 8.83 0.883088 100% Intake z t3014 22.83 9.25 0.850.911 3.4 0.883 97.5 0.889 7.4 -6.9 AA 10 LO 13!5 22 9.25 0.925 1 0.9 0.925 OBB 10 9 14.80 22479 7.99 0.888 1 1 0.799 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 0.1009 PMSD = Percent Minimum Significant Difierence PMSD: 12.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake:

Dunnett's MSD value: 0.1024 Lower PMSD bound determined by USEPA (10th percentile) = 126/6.

PMSD: 12.3 Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data firomEPA's WET Intemaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).

2 USEPA. 001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appcndix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnatin OH.

Page 57 of 100 Fie: sqrt10_050311data.-.xsox Entered by: J. Sumner Reviewed by -.

A -

I ; ý TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated "ES

, Environmental Testing Solutions, Inc.

May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:

Conc-% 1 2 3 4 D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4 0.8488 1.0000 10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4 -0.848 2.410 0.1009 0.8488 1.0000 21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4 0.161 2.410 0.1009 0.8310 0.9791 43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4 -0.161 2.410 0.1009 0.8310 0.9791 86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4 0.795 2.410 0.1009 0.7978 0.9399 100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4 1.231 2.410 0.1009 0.7795 0.9184 Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.0 1) 0.93555665 0.884 -0.827227683 0.41674318 Bartlett's Test indicates equal variances (p = 0.63) 3.45981002 15.0863171 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.10085134 0.12136142 0.00376394 0.00350235 0.406950474 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL(Exp) Skew IC05 75.275 ICI0 >100 ICI5 >100 IC20 >100 IC25 >1001 File: sqnlO_05031 ldata-uv.xlsx IC40 >100 Entered by: J. Sumner IC50 Reviewed by:

Page 5810 f100

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated May 03-10, 2011 EiTS Environmental Testing Solutions, Inc.

Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Cone-% 1 2 3 4 D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4 10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4 21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4 43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4 86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4 100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4 Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4 -1.096 1.943 0.1024 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88838106 0.749 -0.9249599 -0.3380108 F-Test indicates equal variances (p = 0.72) 1.58184743 47.4683456 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.10242295 0.12325264 0.00667012 0.00555646 0.31525472 1,6 Treatments vs D-Control File: sqnll I 050311 data-uv.xlsx Entered by: J. Sumner Reviewed by:

Page 59 of 100

. . ...... . l.. . ............. ........

TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated May 03-10, 2011 S ]Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Environmental Testing Solutions, Inc. Project number: 7068 Concentration Parameter Day 0 Day I Day 2 1 ay 3 1 Bay 4 Day 5 Day 6

_____________________ Initial IFinal Initial IFinal Initial IFinal IInitial IFinal IInitial IFinal Initial IFinal IInitial IFinal PH1 (SU) 7.781 7.78 7.78 7.73 7.891 7.761 7.861 7.741 7.781 7.8J4 7.911 7.661 7.831 7.69 DO (mg/L) 7.81 7.7 7.71 7.8 7.51 7.51 8.01 7.71 7.81 8..0 8.11 7.51 8.01 8.0 Conductivity (lumbos/cot) 324 2 9 0 0 0 1 Control Alkalinity (mg/I. CaCO 3) 6 95 Hardness (mgIL CaCO3 ) 96(09

_______Temperature *C) 24.9 24.71 24.9 24.41 24.9 24.5 24.9 24.9 24.8 24.5 24.84 24.6 24.91 24.61 PH (SU) 7.79 7.781 7.79 7.73 7.89 7.76 7.88 7.76 7.84 7.85 7.92 7.66 7.83 7.70 DO (mg/I.) 7.7 7.7 7.8 7.3 7.7 7.5 7.8 7.8 7.8 8.0 8.1 7.5 7.8 7.8 10.8% Conductivity (jimhos/cm) 306 ~ 310 -286 ý 291 ý 292 297 -- 300

_______Temperature (1C) 25.0 24.7 25.0 24.5 24.9 24.8 25.0 24.6 24.9 24.3 24.9 24.6 24.9 24.6 pH (SU) 7.80 7.77 7.80 7.69 7.891 7.72 7.88 7.76. 7.86 7.86 7.93 7.66 7.83 7.69 2.% DO (mg/L) 7.7 7.7 7.8 73 7.8 7.5 7.8 7.81 7.8 7.9 8.1 7.5 7.7 7.9 2.% Conudctivity (jumbos/cm) 288 -- 293 270 275 28 288 286

_______Temperature (OC) 25.1 24.6 25.0 24.5 24.9 24.6 25.0 24.6 24.9 24.2 24.9 24.7 24.9 24.8 DpH(SU)

O ( mg /L) 7.80 7 .7 7.74 7.79 7.7 7.90 7.70 7.88 7.77 7.87 7.86 7.91 7.65 7.81 7.6 7 .8 7 .9 73 7 .7 7 .5 7 .9 7 .8 79 43.2% Conductivity (pmhos/cm) 252 256 240 24.6 248 79 8 .1 25522 7 .4 7.9 7.8 0

_______Temperature C C) 25.1 24.6 25.0 24.6 24.9 24.6 25.0 24.5 25.0 24.6 24.9 24.7 25.04 24.7 pH(SIJ) 7.79 7.71 7.75 770 7.83 7.691 7.88 7.74 7.84 7.87 7.90 7.62 7.81 7.66 DO (mg/I.) 7.8 7.8 7.9 74 7.9 7.51 7.9 7.7 7.9 8.0 81 7.4 7.81 7.8 86.4% Conductivity (junhos/em) 178 186 179 18 192 197 195

_______Temperature (*C) 25.1 24.6 25.0 24 24.9 24.61 25.0 24.7 25.0 24.5 24.9 24.5 25.01 24.7 pH (SU) 7.73 7.69 7.74 7.62 7.80 7.661 7.88 7.70 7.841 7.861 7.91 7.62 7.791 7.681 DO (mg/L) 8.0 7.7 7.9 7.4 8.0 7.61 7.9 7.8 7.91 7.91 8.1 7.5 7.91 7.91 Conductivity (jimhos/cmn) 154 158 5'5 7 17817 100% Alkalinity (mg/I. CaCti 3 ) 52 615 Hardness (.ng/L CaCO3) 63 69.7 Total Residual Chlorine (mg/I.) <0.10 <0.10101

_______Temperature CC) 25.21 24.71 25.11 24.61 24.91 24.51 25.21 24.71 25.2 24.51 25.01 24.71 25.11 24.71 pH (SU) 7.71J 7.681 7.73 7.66 7.781 7.69 7.78 7.751 7.851 7.851 7.901 7.631 7.781 76 DO (mg/L) 8.01 7.71 7.91 7.51 8.01 8.01 7.91 7.8 8.01 7.81 8.21 7.51 7.91 8.0 Conductivity (tusnhos/cm) 155 15I6 5 6 7 7 100% Intake Alkalinity (mg/I. CaCti 3) 52!96 Hardness (mg/I CaCO31) 63!97 Total Residual Chlorine IL 01 01 <0.100

_______Temperature (C) 12ý 25.11 24.71 25.11 g24.6 j224.91 24.51 25.01 224.6 25.01 24.41 24.81 24.71 25.01 24.7 Page 60 of 100 File: sqn101_050311chem-UV.xls Entered by: S. Evans Reviewed by: A

Page 5 of 6 Species: Pimephalespromelas Date: OS-crn-i\

Client: TVA / Sequoyah Nuclear Plant. Outfall 101. UV-treated Dailv Chemistrv:

Day Parameter Concentration Conductivity CONTROL mho/m)0!3 UV-treated (mg CaCO 3/L) I9 Hardness (mg CaCO 3/L) * "0 Temperature (*C) -*'AA ' I :

pH (S.U.) ,V49 . 9, I.-;ot .13 I * -Y, 1:4,t I 10.8%

DO (mg/L)

Conductivity (Axmhos/cm) :

j 30(j (

Temperature ('CQ 21.6% Conductivity (4mhos/cm)

_ Temperature (°C) 1.1. % I 'LA-A -VAAL.

_i I II -r, -- -I l .. ... .

OtH (S.U.)

I+ ()

II ~

II 1 I 3.vi 1

1W R.,40 I DO (m/L) 43.2% Conductivity (Amho s/cm)

Temperature (°C) 4'.'IS

'U F~. . -

pH (S.U.)

DO (mg/L) 86.4% Conductivity 8 Temperature (°C)

U - -

PH (S.U.)

DO (ma/L)

Conductivity 1 (jrmhos/cm) ____

Alkalinity 100%

(mg CaCO 3 /L) _____

Hardness 63 (mg CaCO 3/L)(mgL)

TR chlorine (mgFL) 2-0, 10 Temperature (°C)

~ . -'-I I=,

pH (S.U.)

DO (mg/L)

Conductivity (A+/-mhos/cn) t 5 100% Alkalinity Intake (mg CaC03/L) 52.

Hardness TRMCaCo rin3 (_agL)

TR chlorine (mg=/I) Ka'. (0 Temperature (°C) ZS, i ... -

Initial II Final I Initial 1- Final I Initial 1t Final I Page 61 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09

Page 6 of 6 I

vienrrMIl T So utlaos, Inc.

Species: Pimephalespromelas Client: TVA / Sea uovah Nuclear Plant* Outfali 101. UJV-treated Dnte~ r~.ni..qA Client TV -/ .... Nu ...... la ...

Plant... ufl 10 U- trae

......... Dae . .. .

I Day I Analyst Concen- Parameter tration PH (S.U.)

DO (mg/L) (3o1 8.0 Conductivity (Lumhos/cm)

CONTROL f9 UV-treated Alkalinity (mg CaCO 3/L)

Hardness (mg CaCO3/L)

Temperature (0Q aA'ItOIL "L*'°t I t *-LA.*

It "7q.B3 "L.

PH (S. U.)

DO (mg/L) 10.8% Conductivity (umhos/cmn) - 1, 1( 1 T.'/ W, 1 -oo 1ý

" " I_

r ITemperature II (mC) pH (S.U.)

DO (mg) 21.6% Conductivity

-(Amhos/cm)

Temperature ('C) P0 1 %q.

7.

- 'U 'U---.. -. - - - .. - -

pH (S.U.) 111. II ý,.-;-- 1 R1,g",11 ".-.- I -: -Qq 11 q 1 "7,81 I1' DBO (MRf) 43.2% Conductivity 9.9 I* am t. *..'i .9~

(iimhos/cm) '.S'?

"4,q "/.t 7. "5.1 Temperature (0 CQ

'U IS-0 a 6,g uI -I/ 01. II q PH (S.U.) I l a DO (mg[L) 86.4% Conductivity

([inmhos-n) *,Lll g "L.q%.0 19"5 lq' Temperature (0C)

S -

pH (S.U.) bbII /o 0 I .R,,4 1I 1S I -7 II .1%2I ".71 Ii3.10 I Conductivity [\i- q Alkalinity1 100%

Hardness TR Chlorine (rag/L)

Temperature (°C) II "**'

Me -I*,L II 14:'S 16.c .. "-0. *l.

- p oH (S.U.) IF '

1-,,--',. -

Sq-, II -4

I-21e) II 7-1,,- I 7-7/B II DO (mg/L) 7 I Conductivity (gtmhos/cm) 100% Alkalinity Intake (Ing CaC0 3 fL)

Hardness

-(mg CaCO3/L) C.O.(0 TR chlorine (mg/L)

Temperature ('C)

-I.. Fina Final I Initial 11 Final In~itial Final I Initial nl -z i Page 62 of 100 .0 JtrO CO M SOP AT20 - Exhibit AT20.3, revision 04-01-09

Page -

k OfJ.

11 ?ae 11 Environmental Testing Solutions, Inc.

11 Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter ii AnalystI Date analyzed 10-IAi I 0 . I1 I odide reagent:

Acid reagent:

[ II

[ 1 qjt q.* l_

Calibration:

Nt:h r a h O .10 mg/L [ 1.00 mmg/L Slo re l SRfrnestandard number I A1s91 J-I ,S59Z4 .

I Note: For samples with a residual chlorine of > 1.0 rag/L, the catibi-ation range must be adjusted to bracket the chlorine levels of the samples.

I Laboratory control standard:

Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

[AS ssa o 0.50 Nf' Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine %RPD = [(S - D) /[(S+D)t2l) x 100 number (m/gLL) (acceptable range =E 10%)

Duplicate D Sample measurements:_________________

Sample Sample I1D Sample characteristics Residual chlorine number _ (gL) m_____________

Reagent Blank --0,0(J03 4 3 1 0I5 PCs i. (30 OW__,__IW. _____ p a.-W.0_

I~*u~*,po.e*o 3 5 ' ive &ti q.C 0,4q5" PUD UL612.ol VA'S- 0 101 rW WI W4-- LJ A-

__ 1,3(a Vote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratory controlstandard:

Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

IW S)9z1 0.50 1/

0,'& TO, 11 Reviewed byIZ Date reviewed Page 63 of 100 SOP C8 - Exhibit C8. 1, revision 09-01-09

Page Page 0V Of F )ET

- Environmental Testing Solutions, Inc.

Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

Matrix: Water, RL ="0.10 mg/L Meter: Accumet Model AR25 pH/Ton Meter Analyst I W Iodide reagent:

Date analyzed 0G.06 11 Acid reagent: [I(NJ qP-4 i Calibration:

0.10 mg/L I 1.00 mg/L I Slope l FReference standard number 7,A ItNSN'L. 1 -'solo Note: For samples with a residual chlorine of" 1.0 mg/IL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory controlstandard:

Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

0.50 o,~ Ou Duplicate sample precision:

Sample Sample 10D Sample characteristics Residual chlorine %RPD ={((S - D) /[(S+D)/12) x 100 number I(mg/L) (acceptable range -: 10%)

4 Duplicate D e t.O .L9 Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number mL Reagent Blank ' DO WTI 2.0o(,0 1_ 6. L

__ OrV-

~~r.~ C-3 P-A7A1 ",0ý 5jk .

~1Q~SSIM. 11 ta Pik.iyqjlJ flOAY L 4rtLu vJL~c <6, woz'.eI Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless oew ise noted.

L aboratory control standard:

Reference standard True value (TV) Measured value (MV) % RS'=NMV /TV x 100 W-66 number Up.eO I oo*

(rag/L) I(rag/L)0. .0 (acceptable range = 90 to 110%)j Reviewed byI Date reviewed I Page 64 of 100 SOP C8 - Exhibit C8.1, revision 09-01-09

Page 22.

I Page

of I I

I . Environmental Testing Solutions, Inc.

Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

I Matrix: Water, RL = 0.10 mg/L Meter: Accumnet Model AR25 pH/Ion Meter Analyst [ fjljj I odide reagent: lst.I*Z +

I Date analyzed [

Calibration:

.jtj 2i%* Acid reagent: I-SwIA&I I I erencestandard number I"L s 0.10 mg/L tk,*

1 1.00 mg/L 5C"IZ-46.9'1.

Slope Aote: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

II Laboratorycontrolstandard:

Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

I -0.50 Duplicate sample precision:

c) 6Lj Sample Sample ID Sample characteristics Residual chlorine %RPD = [(S - D) /[(S+D)/21) x 100 number (mg/L) (acceptable range = : 10%)

4,, Duplicate D o ~o II Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number I (mgIL)

Reagent Blank 40. iy'i,

-NVA SI 0 an&Ag -4r5 a a. 0 000MI I ll..~tC 11______ka 6 QTA~C. ri* . 5 0.LL0 r& G

[I Ii Ii Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

1..

Reviewed by I ýq Date reviewed I OS-41-11 Page 65 of 100 SOP C8 - Exhibit C8. 1, revision 09-01-09

page

___of_.

Page Iof 4 Envoyronmontal TestingSolutions, Inc.

Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst 1,Ii_.- Time initiated F D~o Z Date analyzed Lb .'I j Titrate samples to Time completed L*I I ]

Titrant normality and multiolier determinatinu pH = 4.5 S.U.

pH of Normality Normality (N) of HeSr 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water reference standard ml ml ml 4

= 0.25/E

, =4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220)

= N x 500 Q-"_142iA1 U t-ý35C(Sr, D. Q- 0. 0' 1ql __*.O1___

Laboratory control standard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End. Total Multiplier (Mg CaCO 3/L) (acceptable range (171 CaCO3/L) (ml) ml ml ml =90 to 110%)

TWSS 192-, 100 1oo Ia 1J . q0 c sq..B qol 1' Duplicatesample precision:

Sample Sample ID loe

[Sample volume Begin ak" End Total

]Alkalinity Multiplier (mg CaCO3/L) 9C."

I %RPD=

(a -tb)./[range) 100 number __________ ) ml ml ml ____ ____ (acceptable range = + 10%)

, SSW - -

9.+)2 30 - - -

I Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) 1, number

ýrW'S %93 j

(SV)

CaCO/L) volume (ml) too Begin ml L").

05 -

End ml Total ml Multiplier (mg CaCO 3/L)

Sample alkalinity (B) Measured spike value (MV) % R MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (Mg CaCO3/L) = 75 to 125%) 1 3'4 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number gample ID (ml) ml ml ml Multiplier (mrg CaCO 3/L)

Mc&t_*tII rn4 -ý.*1 1.3s S.q q.q __

46ftzl4 oi1dA 1.3.5 0 .,,(9-0 1

-6Q, 1".5 k~ff __< _______

- -0.0 C6.b*. ) - I . W* - _ __,.

________ SS$IuA __ __ _ 1 Q l 2.l 3.2. _ _ _ 32..

Reviewed by: I]ý& - Date reviewed:

Page 66 of 100 SOP C6 - Exhibit C6. 1, revision 09-01-09

p Page Lk I

D Page Q off SEec'05mw. a TestingSolutions,Inc.

Alkalinity (SM 2320 B)

I Analyst I &7C Date analyzed I DE- 03. jI Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to Time initiated Time completed I Tfrrant nnrmalilv and mulltinls'or datarnlnatiai PH of Normality pH = 4.5 S.U.

Normality (N) Of H2S04 pH Factor or Multiplier I Deionized water 4.5 S.U.

Titrant reference number cNEn--d--

standard number ml E -mlnd Total

"-i--ft-(E)

= (5 ml Na 2 CO 3 x 0.05)/E

.25/E

(acceptable range 20)

= (N x 50000)/N x 100 ml sample 5700ti' I Laboratory control standard.,.

Reference standard True value Sample Alkalinity (MV) % RS = MV /TV X 100 I DIMS*

number tt6 (TV)

(a g CaCO3/L) 100 volume (ml) 100 Begin

)5-ml End ml

?1:5:p.

Total ml C1.1.

Multiplier q.9 (Mg CaCO 3/L) go (acceptable range

=90 to 110%)

.°} ,

I Duplicate sam ple precision:

Sample Alkalinity %RPD=

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) [(S - D)/et(S+D)2l ) x 100 number (ml) ml ml ml I I (acceptable range =:k 10%)

Duplicate (B) -4 D I Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A)

I number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)

(mg CaCO3/L) (ml) ml ml ml 50z ID.

o)(5 3ý..q,

1. .iC..

I Sample alkalinity (B)

(Ing CaCO 3/L)

Measured spike value (MV)

MV = A - B (mg CaCO 3/L)

% R MV / SV x 100 (acceptable range

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Itn CaCO3/L)

O~oiL &t~S IA)S ilOs c:.* fl.,, '." ! jj. Cj" 05M.z It S,%

U24a , 41- o 1 I ____ 9A __

111oS t . 02- 7- 33.-D1 +/-LI__III_ ___4 lUsal~t.C. CI Utz 1 . 1.1l__ __O i as,.c'-- 11o1.1 2-0.LU CO L Wl 10

,(056,1o\ ,,,,e),?.7, ,i

. .I-'I.ot 3¢454, $tlv* 1.' I,\ - & .5 Reviewed by: I KL- IZ Date reviewed: I 0* q -

Page 67 of 100 SOP C6 - Exhibit C6.1, revision 09-01-09

Page _

S Page_.3 of '-

)x.Cnv ronmenta Testing So uions, Inc Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst t Time initiated Date analyzed L.*0.Ii Titrate samples to Time completed pH = 4.5 S.U.

Tifrani nnrmnlntv and mutnlan/ n/ dnats'rinajinn

_pI~-I - ý Nnrmallity'v T Normality (N) of HSO4 pH Factor or Multiplier Deionized Titrant check Begin alld'- mNaCO3 x 0.05)/E = (Nx 50000)/100 water reference standard ml ml =/ 0 ml sample 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) _ x 500 Laboratorycontrolstandard:.

Reference standard True value Sample Alkalinity (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO3 /L) (ml) ml ml ml 90 to 110%)

..5w *?s 100 '00 ,.- I q -.2. C11q 7. g,,-..

Duplicatesam le precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) (S - D) /[(S+D)/21e 1 100 number __ ml) ml ml mll (acceptable range = 10%)

~hb~3A2A~ASga2.-

I Duplicate(B) i 14~1 .3Ž51 1 114.9 Itb 'R* 0 1D + 1 3.qo Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (MngCaCO 3/L)

(imgCaCO3/L) (ml) ml ml ml

,1 IV 5 vD 141

e -. Le 7*e.q )50 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3'L) MV = A - B (acceptable range Ii Sample measurements:_______________

(mg CaCO 3/L) = 75 to 125%)

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml mi Multiplier (mng CaCO 3IL)

(I I1,io.s - 4 3 \ N6D Z5 2 . -3.1f.(.01

26" 13I.' a -

3___

_ -5 -- (44.(, LU oS ,U ,-1 *

R O0 1 1/60 R4 . ,0.1'* tU.i t ns-o _

.-t-

--

40l* .1 .**-9o'**

tel -

5.

______ 7-1TA ZW~sQ AL \&k3L 7-- .2' ii I%0So .7. I____

Reviewed by: I Date reviewed: 10I5 -O i Page 68 of 100 SOP C6 - Exhibit C6.1, revision 09-01-09

F a Page 4-q Page __ ofJ4 SEniwronoetalsTesting Solutions, Inc.

Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst p§;] Time initiated Date analyzed ID . CA -)I Titrate samples to Time completed pH = 4.5 S.U.

Titrant normalitv and multiolier determination:

Normality Normality (N) of H 2SO 4 pH Factor or Multiplier Deicinized Titrant -ch-e-ck- -rgtn- -- *L Tota (5 ml Na2CO3 X0.05)/E =(N x 50000)/ 100 ml sample water reference standard ml ml ml0.25/E N x 500

= 4.5 S.U. number number (E) (acceptable range_=_ _ _____

Laboratory controlstandard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3AL) (ml) ml ml ml = 90 to 110%)

100 100 9_.____._ __.___._______.O_

Duplicate sample precision:

Sample Alkalinity %RPD Sample Sample iD volume Begin End Total Multiplier (mg CaCO3/L) ((S - D)/a(S+D)/21) x 100 number (ml) ml ml ml (acceptable range 10%)

Duplicate (B) I 3 D _._________

Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3 /L)

(mg CaCO3L) (ml) ml mi ml J7 NSSV 3 100 5-J1.* i4 ) qiq ý. 150 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (Mg CaCO/L) MV= A - B (acceptable range (mg CaCO3/L)= 75 to 125%)

59 C1 I Cl. 0010 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (6ig CaCO 3/L)

%I "(13Aa.Cn1* 1 f 31.4 3;4-2 (;b.q A 5e____

11 0S-A'so2'~L- Z 34. .3-4-0 &D____9

_O,.l, , 3?1-,o AR,2. 3.Z ,(L 1 pPec, I ,,t p~jal (nA

(.CI 1.0 121 ýo __0 Id I__ IIwZO ___. 0 Reviewed by: I Date reviewed: b -16 Page 69 of 100 SOP C6 - Exhibit C6. 1, revision 09-01-09

Page Page _ _of 4-Testing Sltons, Ic EnvCiwronmvental Total Hardness (SM 2340 C)

RL = 1.0 mg CaCOJL Analyst V L Time initiated (Oq0Lj

Date analyzed h Time completed jii-Ti trant nnrnaliA and minl ,Pnin, dAta*;i i*

L%)O~T af AI1%-eAWe LIMITS.

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E = (Nx 50000)/50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220) = Nx 1000

'r F,-423 JwSS; TIN f.tI ,

let qi 20,,4oq I Laboratorycontrol standard.

Reference standard number True value (TV)

Sample volume Begin End Total Multiplier Hardness (MV)

(mg CaCO 3/L)

% RS = MV / TV x 100 (acceptable range I *S*9*q (Ing CaC03/L) 40 (ml) 50 ml Cj ;

ml ko-a;7 ml 2.4- 5/^"**

=90 to 110%)

W.1 -2t %A as +81.S 7.

I Duplicatesam e precision:

Sample Sample ID Sample volume Begin End Total Multiplier Hardness (Ing CaCO5IL)

%RPD =

[(S - D)/[(S+D)/211 K 100 number (ml) ml ml ml___1 I oS.os5,1 I Duplicate (B)

HlA 1-33 133*,q 150 1 1.-4 1 2-..._*. s 35 D _________/_

Matrix spike recoverv:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)

_(mg CaCO3/L) (ml) ml ml ml lQ5c 9o -3.3 jJ 1 -. 31-+ 2-04 45 ý+(oJ II Sample hardness (B)

(mng CaCO3/L)

Measured spike value (MV)

MV = A - B (mg CaCO3/L)

% R = MV / SV x 100 (acceptable range

= 75 to 125%)

II Sample measurements:

Sample volume Begin End Total Hardness Ii Sample number TV=ND Sample ID Bank be = 0 mg CaCO3/L)

(should (ml) ml ml P.;

ml 0._cI-'.

Multiplier (mg CaCO3 /L)

_0 Ii 05._-_ ) fY) U4.2. ,_*. __

II t~LZI.II_____A---5 ty4.&.3 4.Z-

.-1, 43.2, -6 st 33.ZA,.__13.-

3'.0o 4.9 Ss II 06S -OZ-1 kiA_ _ _ -- 33__ 24--S II 05.O

.1 IS-_ 5 Li O,*_ _ _ _ _

0.0 L0, 1

4. 1 3.j 3.T I' v_Note:'If_5m g* 70 of-O ~ttrantis used, sample must be diluted. Reviewed by: Date reviewed I oa-O -I"I SOP C7 - Exhibit C7.1, revision 09-01-09

0 Page :35 SE TS,--,

~Environmen a usSousas a nt c Page _* of 4 Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3 L Analyst 7 7 ] Time initiated [ _

Date analyzed [i;3i- i Time completed Titrant normality and multiplier determination:

Titrant NormaV ec- -Bein& I El~d Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml l ml -- -. 2/E = (Nx 50000)/50 ml sample number number (E) (acceptable range =ýu'u0, "020)= Nx 1000 Laboratory control standard:

Reference standard True value jSample Hardness (MV) % RS =MV /TV x 100 [j number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO 3 /L) (ml) ml mj ml =90 to 10%)

Duplicatesa le precision:

ISample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (ng CaCO3/L) {(S - D)/[(S+D)/21) x 100 number _____ ml ml ml ml Duplicate (B) a ) t . 4 I -+. 1 .- D t0 6,6 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3 /L)

(mg CaCO 3/L) (ml) ml ml ml 1'rss Ck4t 1 5 D a-4 11o Lio ~4n 48 KIL(

Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A-B (acceptable range h

(mg CaCO3 /L) 75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness I Sample number TV - Blank Sample ID (should be = 0 mg CaCO3/L)

(ml) ml ml ml Multiplier (mg CaCO3 /L)

II 0mo. .3 8D9 4 l1O$CP In t.OZ- - / -IT . " + I_:f-I____

10Zo Z 32..k 8(07 A4,1 1______

II 05s1. 0-3 k, .+-d ,.-+/--. I'0 lII Oi. 2..1 1$ 43%

- ?.- 5TS 1lO~c~(.0 l. 3 3*5 9 ____5_

1IIOS.O2L-- '4A aw IýT __ _ _3-5 '.o2.cl. _ _

age/If.5mO~i FaOte: 0IU 1 t1t is used, sample must be diluted. Reviewed by: I VI Date reviewed 1 QL" . t SOP C7 - Exhibit C7.1, revision 09-01-09

Page 3 (P I

4 Page .3 of Environmental Tellng Solutions, inc.

4k Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO 3/L Time initiated Analyst Date analyzed e.

05.o:-i i Time completed E I ]

Titrant normality and multiplierdetermination:

Titrant Narmaitv check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml -- -- I = 0.2/E =(N x 50000)/50 ml sample number number (E) le range = 0.0180- 0.0220) = N x 1000 Laboratory control standard:

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mngCaCO3/L) (acceptable range (MagCaCO.L) (m1) mI ml ml = 90 to 110%)

-T5)S 9 1C1 40 50 L*. 2. .- ."-+ 2o.,4 3'57 Q"4's."9. 1 Duplicatesam le precision: Ir .-. el c,**9 .. '. 0 Sample Hardness %RPD =

Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L) ((S - D) /[(S+D)/21) z 100 number (ml) ml ml m

%I Dupi.cat(lB) L 9 2. 12.1 20.4 O S !

Le I Duplicate (B) 1. 11e,131 1 J- IDL _

I I

I Samnie mesuemenft!

. ... T ... ......

m.. ..

Sample volume Begin End Total Hardness I Sample number TV * , -- . Blank '

Sample ID (ml) ml ml ml Multiplier (me CaCO 3/L)

I 1Str.1

rIN*kso-to Im.5 6D..*, *.:-

__-__- i I **-5q 3o.3 4*4+ _

I -. S, __-*\I 30.3 at, -+4,L- _

II II I- 1oAo 10100 1 *. 13M 3.1._._ _ _ _ _

I IIs'.s _ z -49z1 ? 1 41l130Z.02- - c ) iL I3.i ___ _______

I~ ~~ I 72-31U-5I

-Sq ;-y 1 0 105-094, I I -Note: If.,lSml,otit P-age ( 0 UU t is used, sample must be diluted. Reviewed by: 1 -. Date reviewed SOP C7 - Exhibit C7.1, revision 09-01-09

FETS5-Page 3_

Page *L4 offL SEnvIvoflmentaITesting Solutions, Inc.

Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO 31L Analyst[] Time initiated [

Date analyzed Q5-01" t Time Completed "" I.- ZJ Titrant normality and multiplierdetermination:

ck--Nep-°fteclk Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml m = 0.2/E = (Nx 50000)/50 ml sample number number (E) (acceptable ra- .=0 N x 1000 Laboratory controlstandard:

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rag CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml = 90 to 110%)

j55 q jt 40 50 1,.6 q.'-I 2-D q -391 Duplicate sam le precision:

Sample Hardness %RPD(=

Sample Sample ED volume Begin End Total Multiplier ((rg COco 3/L) ((S - D)/L(S+D)/211 x 100 number Ca_ L) (ml) ml ml ml

~ ~J -~ 'fl9.4. 12.1~ ~

D (B)e% Slicate D

-p 1I CQ- -

1280 I to Matrix spike recovens:

eference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3 /L)

(mg CaCO 3/]L) 4... m14 ml ml I -Tjs 1i l40 D Sample hardness (B)

%,~1a1L,. c>

Measured spike value (MV)

-00

%RMV/Sx10 (m=g CC 3 BL) MV=nAkB (acceptable range (119 C*aC*/L) =75 to 125%)

I Sample measurements:_______________

Smlnubr I

Sa I umer Sm c D(ml)

SmlID

$D Sample volume Begin IEnd ml nml Total ml 10, Multiplier Hardness mgCaCO3JL)

TV WD._ _ Blank5 _ _ _ _ '(4_ _ _ _

llnii.~ ~~ cAC ~ Urj-,L +1 z. qo(

12

___ __ __ __ __ __ _ _ _90 3M + sc~

i [=:oo~blfýýt~ft is used, sample must be diluted. Reviewed by: I Date reviewed 105s019- L SOP C7 - Exhibit C7.1, revision 09-01-09

]

r CC-

-%teRfft~b OW~ po~ -01 KAeIWbweSS LOGr.Pa F~ee'-

SuA.t ~ltPage 1of Envionmental Testing Solutions, Inc.

tk f~_~' - eM.

Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO 3/L Analyst Time initiated I oq' I Date analyzed 0 .& . Time completed I Titrant normality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E - (N x 50000)!50 ml sample number number (E) (acceptable tiange = 0.0180 - 0.0220) = NX 1000 I(Neqn (NS6e5 M86.0 Cie.0 Laboratory controlstandard:

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO3 /L) (acceptable range (rag CaCO3/L) (m) ml mi ml ml =90 to 110%)

I 3NlI 40 2I0 ,,. 100o'/,

Duplicatesam le precision:

Sample Hardness %RPD =

Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L) {(S - D) /[(S+D)/21) x 100 number (ml) ml ml m Duplicat_(B) 5q 11A .o 1,1 20.2 41.

I o.E W

Duplicate (B) I IjSj .I I %

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)

TV = ND Blank" (should be =0 mg CaCO3/ 6 _ 6-0. 0.0 0Ai 20.2-

_______ - --. 4-V Q3.L oq~zl.*1.v ,,o'60.q1 3(a.0-A-MS.. I *,3 1

__5.1 qL __

os*. ___ __._

di~i ~- _'

I p~l"f 5& of "pt isused, sample must be diluted. Reviewed by: Date reviewed I "0s,-

SOP C7 - Exhibit C7.1, revision 09-01-09 I

Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix D Reference Toxicant Test and Control Chart Page 75 of 100

Pimephalespromelas T Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.

.a Environmental Testing Solutions, Inc.

1.0 USEPA Control Limits (+ 2 Standard Deviations) 0.9 0.8 0.7 0.6 0.5 t I l I I III I I I I I I I 1.4 I I I I I I I I I I I I I I I I . I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs)

_ 1.2 1.0 U

Ln 0.8 N

>* 0.6 I I I I II - - -- - -- - -- - -- I 0.4 1.4 I I I I I I I I I I I I I I I Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1.2 1.0 0.8 0.6 ..............................................................

0.4 Test date

-. day ICzs = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC25)

-Warning Limits (mean IC 5 25 +/- A10 or SA 7 5) Graphs generated from associated excel spread:

sheet.

......... .Control Limits (mean ICs 25 +/- SA.S SA90' or 2 Standard Deviations) Excelspreads*eet entered by:J,Sumner 5 A. Reviewed by: -I

.* j 5 -. %J1 I %J5.j

I. I d Ii. *.... *********.. - i*.1M ~W -

0 Pimephalespromelas Chronic Reference Toxicant Control Chart EnvironmeutalTesting Solutions, Inc.

State and USEPA Laboratory Laboratory USEPA USEPA 5

Test number Test date 7-day IC. CT Control Limits S&t Warning Limits SA25 Control Limits S&

a Wamrig Limits S&90 Control Limits Cv (9/L KCl) (g/L KCI) CT-25 CT+2a CT- S&W CT + SA.s0 CT-sw CT+s. aT- SA CTa+S_, CT- SA.. CT+ SA.s 1 09-14-10 0.75 2 09-21-10 0.73 0.74 0.02 0.71 0.77 0.09 0.65 0.83 0.16 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 0.02 3 09-28-10 0.65 0.71 0.05 0.60 0.82 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.08 4 09-29-10 0.68 0.70 0.05 0.61 0.79 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.07 5 10-05-10 0.70 0.70 0.04 0.62 0.78 0.08 0.62 0.79 0.15 0.55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.06 6 10-06-10 0.72 0.70 0.04 0.63 0.78 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 7 10-12-10 0.70 0.70 0.03 0.64 0.77 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 8 10-26-10 0.68 0.70 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.05 9 11-02-10 0.70 0.70 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0-55 0.85 0.27 0.43 0.97 0.32 0.39 1.02 0.04 10 11-09-10 0.75 0.71 0.03 0.64 0.77 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.44 0.97 0.32 0.39 1.02 0.05 11 12-07-10 0.77 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 12 01-18-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06 13 01-25-11 0.70 0.72 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.05 14 02-08-11 0.69 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 .0.44 0.99 0.32 0.39 1.04 0.05 15 02-15-11 0.68 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 16 03-08-11 0.71 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 17 03-08-11 0.70 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 18 03-29-11 0.75 0.71 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.05 19 03-31-11 0.75 0.71 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.05 20 05-03-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05 Note: 7-d IC,, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

CT= Central tendency (mean IC25 ).

S = Standard deviation of the ICzsvalues.

Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC2svalues corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

S&. = Standard deviation corresponding to the 10" percentile CV. (SAW0 = 0.12)

SAt = Standard deviation corresponding to the 25' percentile CV. (S,,, = 0.21)

USEPA Control and Warning Limits S,,, = Standard deviation corresponding to the 750, percentile CV.(Sa, = 0.38)

S.= Standard deviation corresponding to the 90" percentile CV. (So = 0.45)

CV= Coefficient of variation of the ICs values.

USEPA.2000. Undersanding and Accounting for Method Variabilityin whole Effluent Toicity ApplicationsUnder the NationalPollutant DischargeElimination Program. EPA-833-R-e0-503.

USEmironmemtal Protection Agemicy, Cinnnati, OH.

File:ppkcclk_0o3elt.xls Page 77 of 100 Organismsobanedrdfmc Aquaoox, Lim Eateredby 1. Serum Hevi.esl by. _.

I Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements

Environrinental Testing Solutions, Inc. Organism Source: Aquatox, Inc.

1.25 I- I I I I I I I I I I I- I I I I I 1.00 0 -2 0.75 W 0.50 0

b LE 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)

II I I I I I I I I I I f I II I -

I I I I I I I I I I I I I I I I I I-30 Kentucky Acceptance Limit (< 30.0%)

0 (uo

-20 I.

- o 10 a.

0 U0 0 I I I I I I I I I* I I I I I I I I I I -

I I I I I I I I I . I I I I I I I I 30 USEPA Upper PMSD Bound (901h percentile < 30.0%)

20 10 QA I ii ii .. i ii................

L v'o vo SO SO vo -0 vo os-o ov" ýV*"';nvý Test date 0 Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

-- Central Tendency (mean Control Growth, CV, or PMSD)

......... Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Page 78 of 100 Graphs generated from associated excel spreadsheet.

Excel spreadsheet enlred by: J. Sumner Reviewed by:

S S Precision of Endpoint Measurements ETS, Environmental Testing Solutions, Inc.

Pimephalespromelas Chronic Reference Toxicant Data Control Sural Control C rolth Mean Test number Test date Survival cr Cv CT MSD PMSD Cr Growth

(%) (g/larvae) for Control Growth (m/arvae) for Control Growth CV (%) (%) for PMSD (%)

1 09-14-10 97.5 0.813 5.9 0.11 13.9 2 09-21-10 100 0.891 0.852 7.5 6.7 0.07 7.5 10.7 3 09-28-10 97.5 0.991 0.898 9.5 7.7 0.11 10.9 10.8 4 09-29-10 100 0.893 0.897 8.5 7.9 0.11 12.1 11.1 5 10-05-10 100 0.912 0.900 8.1 7.9 0.09 9.5 10.8 6 10-06-10 100 0.810 0.885 2.3 7.0 0.06 7.4 10.2 7 10-12-10 100 0.799 0.873 4.1 6.6 0.10 12.0 10.5 8 10-26-10 100 0.779 0.861 10.0 7.0 0.13 16.8 11.3 9 11-02-10 100 0.727 0.846 10.9 7.4 0.11 14.5 11.6 10 11-09-10 100 0.761 0.837 6.3 7.3 0.12 15.4 12.0 11 12-07-10 100 1.080 0.860 8.3 7.4 0.15 13.9 12.2 12 01-18-11 97.5 0.806 0.855 5.8 7.3 0.09 11.0 12.1 13 01-25-11 100 0.608 0.836 4.6 7.1 0.08 13.2 12.2

/

14 02-08-11 100 0.817 0.835 2.6 6.7 0.06 7.8 11.8 15 02-15-11 100 0.701 0.826 3.3 6.5 0.12 16.8 12.2 16 03-08-11 100 0.953 0.834 3.1 6.3 0.08 8.4 11.9 17 03-08-11 100 1.006 0.844 4.7 6.2 0.13 12.7 12.0 18 03-29-11 100 0.829 0.843 6.3 6.2 0.09 10.3 11.9 19 03-31-11 100 0.675 0.834 3.2 6.0 0.09 12.8 11.9 20 05-03-11 100 0.867 0.836 6.3 6.1 0.08 8.8 11.8 Note: CV = Coefficient of variation for control growth.

Lower CVbound determined by USEPA ( 1 0 1h percentile) =3.5%.

Upper CV bound determined by USEPA (9 0 'h percentile) = 20%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10m" percentile) = 12%.

Upper PMSD bound determined by USEPA (90 'hpercentile) = 30%.

CT = Central Tendancy (mean Control Growth, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.

EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-00S. US Environmental Protection Agency, Cincinnati, OH.

File: ppkclcr_05031 l.xlsx Or~n~ bfe$ ( o aox, Inc.

0,-8AQ~bPk nc.Reviewed.4Qhax, Entered by: 3. Sumner by: -+

Page 1 of 5 lET

,* Envl1men l Tmtrg Sulonu s Inc.

I Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephales promelas I Dilution preparationinformation: Comments:

PpKCICR Test Number: _

I KCI Stock LNSS number:

Stock preparation:

Dilution prep (mg/L) 450 Itus.

50 g KCI/L:

Dissolve 50 g KCI in I-L Milli-Q water.

600 750 900 1050

_i_

I Stock volume (mL)

Diluent volume (mL)

Total volume (mL) 9 991 1000 12 988 1000 15 985 1000 18 982 1000 21 979 1000 I Test organism information:

Organism age: 7.. toou.*

Test information:

Randomizing template: k.,i I Date and times organisms were born between:

Organism source:

O1-a2.-ý\ i'.*

_IMY. OS,-n- A Incubator number and shelf location:

Artemia CHM number: CAK ISO..

I Transfer vessel pH = "1.lt S.U.

Drying informationfor weight determination:

Date / Time in oven: o-to.1tt N ip information: Temperature = 0

,A. 1.. C Initial oven temperature: "*(-

Average transfer volume: Date / Time out of oven: ' IiS i.St C. t'*'l*.* Final oven temperature: I C.

Total drying time: __._-_____-_

I Dailyfeeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 0 _._._ " itto ,L tas" J O121L.7.1 A

_______ A%1L. 66 .4..... ....A~*

4.. ito.-A, 3 S.6-1 ISOO os-(n.1%

4 OOI- So L iAS - 6 6 c*M.oCI- I* choo , - -,

6t 140t, Controlinformation: Acceptance criteria Summary of test endpoints:

% Mortality: 1 < 20% 7-day LC50 1q* .S Average weight per initial larvae: 0.-1 NOEC 40o C4 Average weight per surviving larvae: 0.94-1 _>0.25 mg/larvae LOEC q-S J ChV IC25 I & 2 Page 80 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09

r JaEFTS,

.ý Envinsmin,,alTestingSaut annc.n Page 2 of 5 Species: Pimephales Dromelas PpKCICR Test Number: l Survival and Growth Data Day Control 450 mg KCI/L 600 mg KCI/L

,,_A B C D E F G H I J K L 0 10 1 t to I to/CIto0 1/011 C 1 to I to (0 t(0 (to a t t0o to (t0 2 10 to to t(o 0 to /0 /0 /0 /0 (0 3 /0 (to ,I 10 I (0 to 0 /01 to t0 /0 t(o to 4 _ _C_ to Ilo 0Ifo I 10 /o to to to to 6 )* io 1o (0 to to 0 R 0%. ,4%

6 to 10 1 cj (0o I 103 1(0 & '9 A = Pan weight (mg)

Tray color code:: PLUiM Analyst: - '" lq.t 13.4 1t4.1; ,4 .3s4 i .'s( t4-.to* .,5 4.41 IS,.1 4.4t, Date: *A-~4~o~O'i B = Pan + Larvae weight (mg) 21.30 Analyste: M U-90. 2.2.49 28 22-.1 .4'.021,'5 Z.4." 22*.6f 21-.21 21.05 21 50 1..1%

C = Larvae weight (mg) = B - A Hand calculated. -441' tN 201 (,JA -S1 9-06 Analyst: _ 41 ---

Weight per initial number of larvae (mg)

= C / Initial number of larvae Hand calculated. %101 0' 0" 0" 01 Analyst: 01 0' 0* 0O Average weight per initial Percent number of larvae (mg) reduction from control , I 0.1&. 0.15t 7.tl M)/oI Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

I I Page 81 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09

Page 3 of 5 EnvironmritairTtt~inSouionIc Species: Pimephalespromelas PpKCICR Test Number: IS I Survival and Growth Data Day 750 minKC/L 900 mg KCI/L 1050 ml KCI/L 0 M N 0 P Q R S T U V W X

( 10 10

)0 1 10 tq 10 t ; L.

1(

2 tO /0 to /

10 /0 q9 6t R

,~o Ot '&t * " Is~

5 --

6 , 9 . 6,,4 63?A I,4 7_ A (mg)

A = Pan weight Tray color code::. &'u1A Analyst: _ ?A6.

._ ___ 10 13.O01 4-.4t,4.4 13.'- . 13.,eA l4.4f t$.' 4, 13.o3 Date: ____________________

B = Pan + Larvae weight (mg)

Analyst: enJ"* 2. 20A -1 2,.I* 2.*,* i*-.90 10,5 I.5 2.2.,1, l *, 1 .tb-iJ Date: fl..1341 C = Larvae weight (rug) = B - A Hand calculated. S,& "lt*O ",'l- CI'Iq 4* * ' ,*

'4.(T "Aqi I.A&

. , 1,L*

Analyst: _

Weight per initial number of larvae (mg)

= C / Initial number of larvae Hand calculated. ;s '

Analyst: 116gj" 0 0 Ot O. O. 0 &

Average weight per initial Percent number of larvae (mg) reduction o. .,.7. O, . 2.7 from control

(%) ___ _ ___ __.... _

Comment codes: c = clear, d = dead, fg = fungus, k killed, mI missing, sk = sick, sm unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded; Comments:

I I Page 82 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09

L ----- ~-

A Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0

)

Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number PpKCaIt 231 Environmental Testing Solutions, Inc. Test dates: May 03-10, 2011 Dunnett's MSD value: 0.0764 MSD = Minimum Significant Difference PMSD: 8. PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPAs WET Interfaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-9-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

File: ppkclcr 05031l.xcsx Page 83 of 100 Fmtered by: J. Sumcer Organismn obraunedfinmAqnuox. Inca Reniewed by: -*

"ErTS SEslnmtaeslg SouinIn Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:. 5/3/2011 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-82 1-R-02-013 Test Species: PP-Pimephales promelas Comments:

Conc-mg(/L 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 0.9000 1.0000 1.0000 600 0,8000 0.8000 0.9000 0.9000 750 0.9000 0.9000 0.9000 0.9000 900 0.6000 0.6000 0.6000 0.8000 1050 0.3000 0.3000 0.2000 0.2000 Transform: Arcsin Square Root Rank I-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 1 40

600 0.8500 0.8500 1.1781 1.1071 1.2490 6.954 4 10.00 10.00 6 40

750 0.9000 0.9000 1.2490 1.2490 1.2490 0.000 4 10.00 10.00 4 40

900 0.6500 0.6500 0.9413 0.8861 1.1071 11.742 4 10.00 10.00 14 40

'1050 0.2500 0.2500 0.5216 0.4636 0.5796 12.838 4 10.00 10.00 30 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.94239873 0.884 0.46929573 0.83381121 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steels Many-One Rank Test 450 600 519.615242 Treatments vs D-Control Trimmed Spearman-Karber Trim Level EC50 95% CL 0.0%

5,0%

10.0%

20.0%

Auto-25.0% 949.52 891.54 1011.261 File: ppkclcr)05031 1.xlsx Entered by: J. Sumner orPiA9e441,10-gatox,Inc Reviewed by: 4

SETS Statistical Analyses

,* EnvironmensolTestlng Stuos n Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/3/2011 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:

Conc-mg/L 1 2 3 4 D-Control 0,8530 0.8240 0.9470 0.8420 450 0.7160 0.8090 0.7590 0.8440 600 0.6290 0.6640 0.7510 0.8060 750 0.5860 0.7600 0.7730 0.6240 900 0.4340 0.4030 0.4930 0.7440 1050 0.1750 0.1880 0.1220 0.1680 Transform: Untransformed I-Tailed Isotonic Cone-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.8665 1.0000 0.8665 0.8240 0.9470 6.345 4 0.8665 1.0000

450 0.7820 0.9025 0.7820 0.7160 0.8440 7.180 4 2.151 1.943 0.0764 0.7820 0.9025 600 0.7125 0,8223 0.7125 0,6290 0.8060 11.330 4 0.7125 0.8223 750 0.6858 0.7914 0.6858 0.5860 0.7730 13.806 4 0.6858 0.7914 900 0.5185 "0.5984 0.5185 0.4030 0.7440 29.874 4 0.5185 0.5984 1050 0.1633 0.1884 0.1633 0.1220 0.1880 17.593 4 0.1633 0.1884 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p> 0.01) 0.92959601 0.749 0.540073551 -0.97410495 F-Test indicates equal variances (p = 0.97) 1.04289341 47.46834564 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.07635283 0.08811637 0.0142805 0.00308783 0.075051717 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL(Exp) Skew IC05* 230.72 96.49 103.78 614.32 0.9507 ICIO 454.64 81.89 211.64 691.81 -0.0365 ICI5 548.15 86.58 396.44 894.38 0.7530 IC20 708.22 87.46 443.85 854.07 -0.1925 IiCs 782.17 55.25 523.40 935.70 -0.7858 IC40 898.74 32.73 790.71 961.75 -0.2823 ICSO 936.00 22.79 858.25 984.19 -0.3662

indicates IC estimate less than the lowest concentration File: ppkclcr_050311 .xlsx Entered by: J. Sumner OrP,@9q§§bd9Jrn1A9uQbOx. Inc. Reviewed by:*

Page 4 of 5 II T n.s Inc.

"3EnvtmnmentalTestlngSo~utI Species: Pimephalespromelas PpKCICR Test Number: 2,k Daily Chemistry:

Day Concentration Parameter I CONTROL Conductivity (mhos/cm)

Alkalinity 505 I (mg CaCO 3/L)

Hardness (mg CaCO 3/L) ek (Temperature .. vs (0c) II.

pH (S.U.) * -.-

'J.T4 II

~-.-- II -. - -

I 1-', *,

02 II*.-

.5 -. dI-'-'.~-----4I-~---------- II .'t2. iL3+/-3~J DO (mg/L) 450 mg KCI/L Conductivity /Ito J0I~lU At10 1LJ (gmhos/cm) I Temperature 741 1110tb 14 (0C)

PH (S.U.)

S - U 0 - - -iiiiIIII.*

DO (m2IL)

Conductivity 600 mg KCI/L (jmhos/cm) 13 q0 t4o15z0 Temperature

(°C) Is~.I U 'U VA3 pH (S.U.)

DO (mg/L)

Conductivity 750 mg KCI/L (pýrnhos/cm) I 1011%2 1(540 Temperature (0C)

_ _ _ _ --- I- -*.

DO mj 900 mg KCI/L Conductivity Temperature & "44.1 (c), .

pH (S.U.)

hn f,., -/I I

-q'(91 I'sk I'm qlt__ , -ý ILIUtgt.,

Conductivity ?AID"71 U 1050 mg KCI/L (gmhos/cm) I Temperature (0c)

STOCK Conductivity

___________(jmhos/cm) ~ ot Initial II Page 86 of 100 SOP AT21 - Exhibit AT21.1, revision 04-01-09

F" S

Page 5 of 5 3

SollnkIonc..

ý) EnvimnMentaiTesting Species: Pimephalespromelas PpKCICR Test Number: ___ .-

Day 3 4 5 6

___________Analyst Concentration Parameter Conductivity (Amhos/cm).3z CONTROL Alkalinity a k, I (mg CaCO3/L)

Hardness

_(mg CaCO 3/L)

Temperature

___ _ _ pH("., I .-i$l-.. ".,) iA.! 511" -M -3q" " do 450 mg KCIL Conductivity 60g CI DO (mg/L)

Temperature m'*

Conductivity *'*"M. i %].1. *73 7* .5

l U-1

_pH (0C) _:1.9_. "-W 900 mg KCIL Conductivity 45 ..

(iLmhos/cm)

Temperature " * ".4 V . i"-' ". . " .3

_(C)

DO (mgIL) -1 9-.

750 mg KCI/L Conductivity I (Amhos/cm)_t

-pH(S.U.)

DO (mg/L) .

Temperature "L.4.r " .. "

(°C) pH - - 9 Initial Final Initial Final I nitial Final Initial 1 Final I Page 87 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09

O Ceriodaphniadubia

D Chronic Reference Toxicant. Control Chart Environmental Testing Solutions, Inc.

I I I C on oI I I

(+ 2I Standard I I I I I I 1.14 USEPA Control Limits Deviations) 1.12 1.10 .. .

,a°................'....................................

1.08 1.06 1.04 1.02 K

I I I I I I I I I I I I I I I I I I 2.5 S I II I I I I I I I I I

I I

USEPA Warning and Control Limits (75th and 90th Percentile CVs)

N 2.0 1.5 1.0 N

0.5 N

II I I I I I I I I. I I I I . I I I I 1.4 I I I I II I" I I I I I I I I I I I I '

1.3 Laboratory Warning and ControlLimits (10 and 25 Percentile CVs) 1.2 1.1 1.0 0.9 .........................................

0.8 Test date

7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.

Central Tendency (mean IC25)

Warning Limits (mean IC2 5 +/- SA.1 0 or SA.75)

......... Control Limits (mean IC 25 +/- S A25, SA90, or 2 Standard Deviations)

Graphs generated from associated excel spreadsheet.

Page 88 of 100 Excelspreadsheet mrtered by: J.Sumner Reviewed by:

Ceriodaphniadubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.

State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day JC2s. CT S Control Limits S.:o Warning Limits SA.25 Control Limits SA.s" Warning Limits S" Control Limits Cv (g/L NaCI) (g/L NaCI) CT-2S CT+2S CT - SAJO CT + SAl. CT - SA-2 CT + SA2u CT - SAMs CT + SA.7s CT- Sse CT + S,.

1 12=08-09 1.05 2 01-05-10 1.08 1.07 0.02 1.02 1.11 0.09 0.98 1:15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.02 3 02-02-10 1.07 1.07 0.02 1.04 1.10 0.09 0.98 1.16 0.18 0-89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.02 4 03-02-10 1.08 1.07 0.01 1.04 . 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 5 04-06-10 1.05 1.07 0.01 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 05-04-10 1.09 1.07 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.02 7 06-08-10 1.07 1.07 0.02 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 8 07-13-10 1.07 1.07 0.01 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59. 1.55 0.66 0.41 1.74 0.01 9 08-03-10 1.06 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 10 09-14-10 1.07 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 11 10-05-10 1.08 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 12 10-05-10 1.08 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 13 11-02-10 1.09 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.67 0.41 1.74 0.01 14 12-07-10 1.06 1.07' 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.56 0.66 .0.41 1.74 0.01 15 01-18-11 1.06 1.07 0.01 1.05 1.10 0.09. 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 16 02-08-1I 1.06 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 17 03-08-1 1 1.05 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 18 04-05-11 1.06 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.1.8 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 19 04-05-Il 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 "1.73 0.01 20 05-03-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.73 1.55 0.66 0.41 0.01 Nole: 7-d ICm = 7-day 25% inhibition concentration. An estimation of the concentration ofl'odium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.

CT ý Central tendency (mean IC25).

S = Standard deviation of the IC2 5 values.

Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC 25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

SA.jO= Standard deviation corresponding to the 10"' percentile CV. (SA& =0.08)

SA.zs = Standard deviation corresponding to the 25" percentile CV. (SA.2s =0.17)

USEPA Control and Warning Limits SA.S = Standard deviation corresponding to the 75"' percentile CV. (SAMS = 0.45)

SA9o = Standard deviation corresponding to the 90"h percentile CV. (SA.9o = 0.62)

CV = Coefficient of variation of the IC25 values.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.

Page 89 of 100 Fie: d~aCC b5y Is-l H by--.Sutn

EnT Environmental Testing Solutions. Inc.

Ceriodaphniadubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 0

0 35 0 T

0 30 0

I-25 0

I.' .20 0

0 Q 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)

I i i I i i i i i I Ii ti . i i :

. . . . .I I I I I I I I..

. 40 North Carolina Acceptance Limit (< 40.0%)

30 Kentucky Acceptance Limit (< 30.0%)

o 20

Q10 50 - I I _I _-I I I I I I I I I- - -

USEPA Upper PMSD Bound (90th percentile < 47.0%)

40 30 20 10

. I I

................. .. . I ..... .................. . .... .. ..

I.

A1 V

k,.V% sm0-11S"oNNAl'oýOI.v% %VVS'NN%IVA*"li0.",

N tNý0-11.S"ilN 011,SAN

%-511 Test date

- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

-- - Central Tendency (mean Control Reproduction, CV, or PMSD)

......... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Graphs generated from associated excel spreadsheet.

Page 90 of 100 Excelspreadsheet ejter d by: J. Sumner Reviewed by: 7

Precision of Endpoint Measurements 0

Ceriodaphniadubia Chronic Reference Toxicant Data SEnvironmental Testing Solutions, Inc.

est TControl Control Mean CT CV CT MSD PMSD CT number T Survival Reproduction for Control Mean for Control

(%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%)

(offspring/female) Cy (%)

1 12-08-09 100 35.2 4.6 2.0 5.8 2 01-05-10 100 31.1 33.2 5.8 5.2 2.3 7.3 6.6 3 02-02-10 100 31.9 32.7 3.5 4.6 2.6 8.3 7.1 4 03-02-10 100 31.0 32.3 6.3 5.0 2.4 7.7 7.3 5 04-06-10 100 32.7 32.4 6.3 5.3 2.4 7'4 7.3 6 05-04-10 100 31.0 32.2 4.6 5.2 2.3 7.4 7.3 7 06-08-10 100 33.1 32.3 6.6 5.4 2.4 7.2 7.3 8 07-13-10 100 31.6 32.2 4.8 5.3 2.3 7.2 7.3 9 08-03-10 100 28.2 31.8 4.7 5.2 1.8 6.2 7.2 10 09-14-10 100 32.9 31.9 8.7 5.6 3.0 9.2 7.4 11 10-05-10 100 30.2 31.7 7.6 5.7 2.0 6.6 7.3 12 10-05-10 100 30.8 31.6 4.0 5.6 2.2 7.2 7.3 13 11-02-10 100 30.5 31.6 4.9 5.6 2.0 6.6 7.2 14 12-07-10 100 32.2 31.6 4.8 5.5 2.0 6.2 7.2 15 01-18-11 100 31.9 31.6 6.3 5.6 2.3 7.1 7.2 16 02-08-11 100 30.7 31.6 6.9 5.6 1.8 5.7 7.1 17 03-08-11 100 32.6 31.6 4.8 5.6 2.0 6.0 7.0 18 04-05-11 100 32.8 31.7 5.9 5.6 2.2 6.8 7.0 19 04-05-11 100 33.0 31.8 4.5 5.6 1.9 5.7 6.9 20 05-03-11 100 30.9 31.7 6.7 5.6 2.5 8.1 7.0 Note: CV = Coefficient of variation for control reproduction.

Lower CV bound determined by USEPA (10I' percentile) = 8.9%.

Upper CV bound determined by USEPA (90&' percentile) = 42%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) = 13%.

Upper PMSD bound determined by USEPA (90th percentile) = 47%.

CT Central Tendancy (Mean Control Reproduction, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

File: CdNaCICR_05031 l.xlsx Entered by: J. Stunner Page 91 of 100 Reviewed by:_4 1

Fr - Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphniadubia CdNaCICR #: 11O Dilutionpreparationinformation: Comments:

NaCI Stock INSS number: UIWS_ 1131 Stock preparation: 100 g NaCI/L:

Dissolve 50 g NaCI in 500 mL Milli-Q water.

Dilution brep (rg/L) 600 800 1000 1200 1400 Stock volume (mLr 9 12 15 18' 21 N Diluent volume rmL) 1491 1488 1485 1482 1479 ....

Total volume (mL) 1500 1500 1500 1500 1500 1 Test organism source information: Test information:

Organism age: < 24-hours old Randomizing template color: (l.k Date and times organisms were born 0*O%4j% - n'*vri VLOG

%* Incubator number and shelf between: location:

Culture board: A _ __ _

Replicate number: 1 21 3 1 4 1 s 1 6 1 7 08 1 9 ito YWT batch:

Culture board cup number: S "1 1 '

lq SI 1I. LtI _A _______S__

ITransfer vessel information: PH = 2_ S.U. Temperature = .L 'C Selenastrum batch: O4 z."il Average transfer volume (mL): 1A. 0,4.M-4 Daily renewal information:

Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 Qf-1 'S 01TL,60 A *NO 1 eI6q- I I S t. QS l1 F 2 s.-1. IIIus 0 d 6s____

____ I70 n,.a-S. I 5 _______1 1200 L CG I-I-A -

6 .15 '1 Control information: Acceptance criteria Summary of test endpoints:

% of Male Adults: 67, < 20% 7-day LC50 Won II % Adults having 3r Broods:

% Mortality:

Mean Offspring/Female:

fo?

0*,

a 80%

520%

15.0 offspring/female NOEC LOEC ChV

&00

/000 gig, I

%CV: <40.0 % IC2s5 0 ,K(0 Page 92 of 100 SOP AT 14 - Exhibit AT 14.1, revision 04-01-09

Page 2 of 6 3

,. E vbrnm t11Sotfln.Tlo Tn sI Ac.

Species:. Ceriodaphniadubia CdNaCICR : VZ CONTROL Survival and Reproduction.Data Replicate number Day, 1 2 3 4 5 6 7 8 9 10 I Young produced Q 0 C). . 0 C,._ 0 0 0 Adult mortality \ _

Adult mortality I _ - j 3 Young produced I.~ 0 0 0 0 C I0 Adult mortality _ +/- . I .. \--

'". \-- *-\-- '" I'-

4 Young produced &4. I] '\4 ' .. A.

Adult mortality - L~ t-- L-Adult mortality L.. .. .- .

6 [ Young produced 0 == 0 " 0 0 0 Adult mortality. t...... _ _ . _ * ',..

7 Young produced 1 1b 14*t, I to I I Total young produced S. -6 "I2. 3 L 5. o 32. 7-- 0 Final Adult Mortality - s...

W * * .

X for 3rVBroods y_ >- ,- ><. >52 1 >1 > <-

Mote: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

S%Mortality: I 01.

Mean Offsprin emale: .*'.

I 600 m NaC IL Survival and ReproductionData I

Day I

2 I

Young produced Adult mortality Young produced 1

0 2

0-0 3

()

\-

0

\

4

[

0 Replicate number 0

5 i(0 6

0101010 L.

7 L

8 I--..

. L 9

0f0 10 k-_

0 I 3 Adult mortality Young produced Adult mortality _.

0 IQ 0

[

0 0

1_. I 10 0 L4_.

. 4" I 4 5

Young produced Adult mortality F-Young produced 1, C

[.j*

_ I,

[ c iL I

ji I L.

I I\

jLJL...

IL JL Adult mortality jI- - L U~_

6 Young produced 100 C I C) n (o) oL

[Adult mortality C T' j L.-J E U-- JL 7~~ Young produced t V!5 % I I% tip %- Cb ]

I Total young produced 2A 1\

" zl. '51 I

\

'-i ,

I

,3 I Final Adult Mortality .L k...

Mote: Adult mortality (L= live, D = dead), SB = split brood (single brood split between two days), CO = carry ovei (offspring carried over with adult during transfer).

I Concentration:

% Mortality: 07.

Mean OffsDrina/Female: I 4n.

% Reduction from Control: 1OW. 1 I Page 93 of 100 SOP AT 14 - Exhibit AT 14. 1, revision 04-01-09

Page 3 of 6

)

)_

Species: Ceriodaphniadubia CdNaCICR #: t?.c) 800 m NaCI/L Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced ...cQ 6 O 1 0 00 Adult mortality _ _ _ - _ ___ _ __ ._

Young produced 0I 0 Adult mortality __._ __ L L._

4 Young produced ., I- -

Adult mortality t4I 5 Young produced iL2 10o to 10 7Z. 0 10 10D Adult mortality ,..L_._ .

Adult mortality V_ t_.'-

7 j Young produced I' S \1IS% t ) I%. it. I* " 1 4I :' Sq Total young produced 3* * $ , . .*b 3 1 7"]

1Final Adult Mortality -- K-1 %.

Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

% Mortality: 07, Mean Offspring/Female: 21-1

% Reduction from Control: 3.27.

I 1000 mg NaCI/L Survival and ReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 10 Adult mortality Adult mortality=-=. j L ...

3 Young produced Adult mortality [ L Li Young produced fl fl[Q otO1, Adult mortillity

Adult mortality IL1 LJ L -- --

6 Young produced _0I0 7

Adult mortality Young produced Total young produced t -

I - J ... I JL IS t...

=

...1*".

f_0==. -

Total y MortaVity.

pdu c vi 3b 1- 2-1 1Final Adult Mortality = -j .-. %

'- . '- i l:

Note: Adult mortality (L = live, D = dead), SlB = split brood (single brood split between two days), UU = carry over (offspring carried over with adult during transfer).

Concentration:

%Mortality: 0.7 Mean Offspring/Female: 2t.0

% Reduction from Control: '.4 7.

Page 94 of 100 SOP AT14 - Exhibit AT14.1, revision 04-01-09

I *

Page 4 of 6 I Species: Ceriodaphniadubia CdNaCICR #: 1rc:3 I 1200 ma NaCI/L Day _1 Young produced - c-2 3 Survival andReproduction Data 4

Replicate number C

5

)

6 1 7 C 8 -

9 10 1

Adultmortality Young produced C) o l p- C>

- .. c' ._ iC _ _

Adult mortality U- , - [ 1-b I

'- , - - ] .

I 3 4

Young produced Adult mortality Young produced V-p 0 I. 1 I Q.

L c-I U

. I .. I 0

I "" Adult mortality 4_ * ,%-- _ . "

I L 5 Adult mortality Young produced Ik 3*__

L.L U ' JU It [ ... V -

I 6 Adult mortality Young produced 0. 0AI I Cl- . 0I' j 0I1~

-I 7 [Young produced 0*1 A~ 12 0 Q '

Total young produced I 1- 1

-1 '* '

j I Final Adult Mortality l *I

I * - - L- i...

Note: Adult mortality (L = live, D dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer). __Concentration:_ [

I. IConcentration:

% Mortalit:

[ Mean Offspring/Female:

% Reduction from Control:

I &."

W.

I 51.01.

1400 mg NaCI/L Survival and ReproductionData Replicate number Day 2 J 3 4 5 6 7 8 9 10 I 2 Young o produced Adult mortality Young produced Adult mortality 10

(* [ C 050 ..

0 _

0*

0*_

t-0V...

) 0 0L,-

(

0.

(

0'.

I 33 prdue I

Adult mortality

-I Yon J. .

L..c L- o0 L (..0 I Adult mortaffity Young produced Adult mortality V,

0 CC Q-L 0 7--

L L 0 1 0 L- L

.=:~

I 6 Young produced Adult mortality !

I . 0 U-J J I

£ 0

U. I- 0 Co %4 I

7 - Young prodedcc -k - 7 Total young produced 0. 1 T4! 21 . 6 Final Adult Mortality . . C___ " L = =

I Note: Adult mortality (L= live, D dead), SB split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer).

Concentration:

% Mortality: 1.

Mean Offspring/Female: 4L,3

% Reduction from Control: A .17-I Page 95 of 100 SOP AT 14 - Exhibit ATI 4.1, revision 04-01-09

o Verification of CeriodaphniaReproduction Totals

3) Environmental Testing Solution s, Inc.

Control 1000 mg NaC/L-Replicate number Total Re plicate number Day 1 2 3 4 5 6 7 8 9 10 Day 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 43 401-2 40 10 3 503 401 402 12 6 402 402 30 01 41 4 3 3 4 4 3 3 3 5 3 3 34 5 12 12 I0 13 II 12 12 12 ,12 10 116 5 10 12 9 12 10 10 9 !11 10 10 103 6 0 0 '0 0 .0 0 0 0 0 0 0 6 0 0 000 0 0 0 0 0 0 7 16 16 13 14 16 16 15 16 13 17 152 7 1 15 13 13 15 14 14 14 15 14 143 Total 32 32 26 32 31 32 33 32 29 30 309 Total 29 30 126 29 28 27 26 30 28 27 280 600 mg NaCM/L 1200 mg NaCI/L Replicate number Total Day Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 2 1 3 4 1 5 1 6 7 1 8 1 9 10 1 0 0 0 0 0 0 0 1 0 0 0 0

! 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 .0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 00 0 0 0 0 4 5 3 3 4 3 4 5 4 4 4 39 4 2 3 3 4 3 1 3 2 2 4 27 5 13 10 12 12 10 12 11 11 I 12' 114 5 8 5 5 7 4 6 4 5 4 7 55 6 0 ,0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 18 15 15 16 13 17 15 15 16 15 155. 7 9 8 6 3 5 0 6 4 3 7 51 Total 36 28 30 32 26 33 31 30 31 31 308 Total 19 16 14 14 12 7 13 11 9 18 133 800 iug NaCI/L 1400 mg NaCI/L Replicate number Total Day Re plicate number Total D

1 2 13 4 5 16 7 18 19 10 1 2 3 4 5 6 7 8 9 10

- 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 3 4 4 5 4 4 4 3 3 38 4 1 0 0 I 0 0 0 2 0 0 4 5 12 10 10 II 10 12 10 10 10 13 108 5 0 2 1 0 2 2 0 0 1 3 II 0 0 0 0 0 0 0 0 0 6 3 0 0 0 0 0 3 0 0 0 6 7 15 15 17 15 13 16 16 17 14 15 153 7 4 1 0 4 2 2 I 5 0 3 22 Total 31 28 31 30 28 32 30 31 27 31 299 Total 8 3 I 5 4 4 4 7 I 6 43 File: CdNaCICR 050311.xlsx Page 96 of 100 Entered by: J. Sumner Reviewed by: O

E Ceriodaphniadubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0

.* EnvironmentalITesting Solutions, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #120 Test dates: May 03-10,2011 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%)

Control 32 32 26 32 31 32 33 32 29 30 100 30.9 6.7 Not applicable 600 36 28 30 32 26 33 31 30 31 31 100 30.8 8.8 0.3 S00 31 28 31 30 28 32 30 31 27 31! 100 29.9 5.6 3.2 1000 29 30 26 29 28 27 26 30 28 27 100 28.0 5.3 9.4 1200 19 16 14 14 12 7 13 11 9 18 100 13.3 28.4 57.0 1400 8 3 I 5 4 4 4 7 1 6 100 4.3 53.8 86.1 Dunnett's MSD value: 2.511 MSD = Minimum Significant Difference PMSD: 8.1 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) = 13%.

Upper PMSD bound determined by USEPA (90th percentile) = 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001b).

USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82 I-B-01-004 and EPA-82 I-B-01-005.

US Environmental Protection Agency, Cincinnati, OH.

File: CdNaCICR_050311.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Spreadsheet entered by: J. Surpner Page 97 of 100 Reviewed by:

VETS S

0 0

Statistical Analyses

~ Environmental Testing Solutions, Inc.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/3/2011 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 5/10/2011 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments:

Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 32.000 32.000 26.000 32.000 31.000 32.000 33.000 32.000 29.000 30.000 600 36.000 28.000 30.000 32.000 26.000 33.000 31.000 30.000 31.000 31.000 800 31.000 28.000 31.000 30.000 28.000 32.000 30.000 31.000 27.000 31.000 1000 29.000 30.000 26.000 29.000 28.000 27.000 26.000 30.000 28.000 27.000 1200 19.000 16.000 14.000 14.000 12.000 7.000 13.000 11.000 9.000 18.000 1400 8.000 3.000 1.000 5.000 4.000 4.000 4.000 7.000 1.000 6.000 Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 30.900 1.0000 30.900 26.000 33.000 6.728 10 30.900 1.0000 600 30.800 0.9968 30.800 26.000 36.000 8.766 10 0.091 2.287 2.511 30.800 0.9968 800 29.900 0.9676 29.900 27.000 32.000 5.563 10 0.911 2.287 2.511 29.900 0.9676

1000 28.000 0.9061 28.000 26.000 30.000 5.324 10 2.641 2.287 2.511 28.000 0.9061

1200 13.300 0.4304 13.300 7.000 19.000 28.366 10 16.027 2.287 2.511 13.300 0.4304

1400 4.300 0.1392 4.300 1.000 8.000 53.763 10 24.223 2.287 2.511 4.300 0.1392 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.82175648 1.035 -0.1981616 0.6557231 I Bartlett's Test indicates equal variances (p = 0.07) 10.1990776 15.0863171 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 1000 894.427191 2.51109516 0.08126522 1279.06667 6.02962963 3.3E-34 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL Skew IC05 857.368421 101.848477 536.115476 1000.42718 -1.5395 ICI0 1002.58503 37.7430109 902.799145 1019.00076 -1.1222 IC15 1023.60544 9.16420366 1004.92346 1039.04671 0.1840 IC20 1044.62585 8.95991641 1027.21063 1060.90996 0.1797 lIC25 1065.64626 9.15526908 1047.78147 1082.77122 0.19211 IC40 1128.70748 11.7423916 1107.58171 1149.34211 0.4319 IC50 1170.7483 14.442622 1144.89854 1199.51128 0.5658 File: CdNaCICR 050311 .xlsx Entered by: J. Sumner Reviewed by:

Page 98 of 100

Page 5 of 6 II ~

E~Imn,.flt.I~IIflS.I..fh,.Lt,~.

Species: Ceriodaphniadubia CdNaCICR #: _

Daily Chemistay: _ _ _ _ _ _ _ _ __ _ _ _ _ _ _ _ _ _

I Day I I 0 I f I

Concentration Parameter i

DO (S.U.)

DO (me/L)

I Conductivity (Amhos/cm)

-133

?a 105 CONTROL Alkalinity I (mg CaCO 3/L)

Hardness (mg CaCOd/L)

O5 \

4-Temperature (C) 'AA1- A_ LS2 .{i w t..'

I. P ~ qi ~. I-i (S.U.

DO (mg/L)

I.. '14.8eo L JL Jr1 1 1 J 600 mg NaCl/L FConductivity [

(jm 0,s/m Temperature I (0C)

H (S.U.) 4 ~~.27 4 4~~ 7.07, 1 -27T IrMOR 4 -L-~-3--..4I.J Kki~i iR1U~ I DO (mg/L)

I 800 mg NaCI/L Conductivity (gmhos/cm)

Temperature 1s1 I a (0C)

PH (S.U.)

a-Il-i I i-.1 1000 mg NaCI/L DO (mna/L)

Conductivity

(~-nolmb ;

UA Temperature

(°C)

IUS -o . 7.q. I I p (0c) pH (S.U.)

DO (ma.L) p 14 (3 4

11 'R(.

S-1 ,91 11 1200 mg NaCI/L Conductivity (gtmh s/cm)

Temperature iS. o

("C) iSq Ml'4.9 o I pH (0c)~i (S.U.)

DO (ma/L)

I I

! 1400 mg NaCI/L Conductivity (P hos/CM)

Temperature Viot 0

(°C) 2S.0 07jq. OLS-0 r7.q 44 STOCK Conductivity (Amhos/cm) 1010 /o00 -

Initial .11 Final Initial 11 Final I Initial Final I Page 99 of 100 SOP AT14 - Exhibit AT14.i, revision 04-01-09

Page 6 of 6 Species: Ceriodaphniadubia CdNaCICR 1:10..

Ii 3 4

~Day 5

6 I PY

___ __ _ _Analyst L aSI Concentration Parameter pH (S.U.) .S Iwo 1.0 5 I C ond uctiv ity (jgimhos/cm)

DO(m/)

3' I ,1 1.!--

2.

CONTROL Alkalinity a I (mg CaCO 3/]L)

Hardness (mg CaCO3/L) i ir\

Temperature V pH (S.U.) +/- -

DO (mg/L) 1-.ý<.

600 mg NaCI/L Conductivity fflo 14w IIHO (pinhos/cm)

Temperature 800 mg NaCl/L Conductivity

.)

. qo Temperature US.A pM0os/cm)

H0 (s .u.) . * .1 L-

2 J3 2OoO __l _

Temperature ".l pH (S.U.) "

_._ U a

._7__1__. 1.9 DO (mg/L) ) 1a0 1200 mg NaCi/L Conductivity Conductivity l* Jt "L1 i(

1000 mngNaCI/L .. mhos/cm)

DO T em(perature g/L) -5 I-A S 1- .I 17Y.I.

- O..C) - --- -- ..--. -. 14. t 2 A M4 1400 mg NaCVL Conductivity 0~o2~30 Temperature V~4.A 4.1 IN.'& 2,4.1 Is.6

-(0c)-

Initial 11 Final Initial 11 Final I Initial 1 Final I Initial Final I I Page 100 of 100 SOP AT14 - Exhibit AT14.1, revision 04-01-09

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01)

Address P.O. BOX 200 (INTEROFFICE OPS-jN-SQN) TN0026450 IIL 103 G F-FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER IDISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Facility TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY MANITAIrINr. DPiD~fnf I EFFLUENT FromI YEAR 05 01

-DAY To 11 M05 IK *-* NO DISCHARGE ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form.

PARAMETER

PARAMETER QUANTITY OR QUANTITY OR LOADING LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE

7 8 12 0 13/31 GRAB MEASUREMENT 00400 1 0 PERMIT 6 9 SU THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 13 14 19 0 2 / 31 GRAB MEASUREMENT 00530 1 0 PERMIT 30 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE <6 <6 19 0 2 / 31 GRAB MEASUREMENT 00556 1 0 PERMIT, 15 20 MGIL TWICE/. GRAB EFFLUENT GROSS REQUIREMENT , MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.985 1.303 03 ** 0 31 /31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD SEE RCORDR EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX PERMIT SAMPLE MEASUREMENT PERMIT.

REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my r-.1*".. TELEPHONE DATE SDlirection gather or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vic&-#r*ident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties-for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE ncluding the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceall attachmentshere EPA Form 3320-1 (REV 3199) Previous editionsmay be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR). (SUBR 01)

Name TVA - SEOUOYAH NUCLEAR PLANT OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE OPS-5N-SON) TN0026450 I 110 G I F-FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facilitv TVA - SEOUOYAH _NUCLEAR PLANT Locatio HAMILTON COUNTY SMONITORING, PERIOD IEFFLUENT I YroA I MO TODAY Y ATTN: Stephanie A. Howard From l 111 T05 01 1 TO I11 I05 I31I NO DISCHARGE Fjx ...

NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 1 0 PERMIT

REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS TEMPERATURE, WATER DEG. SAMPLE **

MEASUREMENT 04 CENTIGRADE 00010 Z 0 PERMIT 30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE UPSTRM DEG.C MEASUREMENT 04 00016 1 0 PERMIT 5 DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE 03***

MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1 0 PERMIT 0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE 04***

MEASUREMENT 04 CHANGE 82234 1 0 PERMIT 2 DEG C ******** CONTIN CALCTD REQUIREMENT . DIYM EFFLUENT GROSS VALUE DAILY MX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments werethat prepared under qualified my personnel TELEPHONE DATE direction or supervision in accordance with a system designed to assure e Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vic'4sident information, the information submitted is, to the best of my knowledge and belief, true, accurate, y 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I A ZRR COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceall attachmentshere No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0 004 Address p.O.BX 2000 (INTEROFFICE OPS-5N-SQN)

F - FINAL SODDY - DAISYTNM 7384 PERMIT NUMBER 1 DISCHARGE NUMBER RECYCLED COOLING WATER Facility TA - SEQUOYAH NUCLEAR PLANT EFFLUENT Locatio HAMILTON COUNTY o

-YEAR I MO MONmITRING IDAY I PE:RIOD YA- f IDY FromL 11 1 05 o01 1 To [ 1I ots 131 *** NO DISCHARGE @ ...

ATTN: Stephanie A. Howard NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS UNITS ANALYSIS MINIMUM AVERAGE MAXIMUM IC25 STiATRE 7DAY CHR SAMPLE

23 CERIOD APHNIA MEASUREMENT TRP3B 1 0 0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUE NT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STAATRE 7DAY CHR SAMPLE 23 PIMEPH ALES MEASUREMENT 23 TRP6C 1 0 0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUE NT GROSS VALUE REQUIREMENT

_____ ________ _______ MINIMUM ___________ _____ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT F I I +/- F F +

SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Mairection or supervision in accordance with a system designed to assure that qualified personnel Y 1 ' %/ { TELEPHONE DATE Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice dent information, the information submitted is, to the best of my knowledge and belief, true, accurate, _ y_ _ - _ _ _ _423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR TYPED OR PRINTED CODE MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachmentshere No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page I of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE OPS-5N-SON) TN0026450 t 118G F-FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUMBERI WASTEWATER & STORM WATER Facility TVA - SEQUOYAHNUCLEAR PLANT amf~Ifn~rfr~l I EFFLUENT Locatio HAMILTON COUN-TY. MILMLLVELULNU Itfr-EUMU ATTN: Stephanie A. Howard Fromi1 I 1 I TO YEARI 1 28y NO DISCHARGE --1

NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT 19 00300 1 0 PERMIT 2 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 PERMIT 100 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 25 00545 1 0 PERMIT

MUL ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03* **

MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD **ONCE/ ESTIMA REQUIREMENT EFFLUENT GROSS MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel t Michael D. Skaggs properly persons gather and evaluate who manage the information the system, submitted.

or those persons Based directly on my inquiry responsible of the person for gathering the or Sequoyah Site84Vice01 11s06nt information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE -

including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED ORPRINTED OFFICER____OR__AUTHORIZED_____A__ENT__AREA__ NUMBER____YEAR__MO__DAY_

CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceall attachmentshere During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

editions maybe used Page 1 of 1 Previous EPA Form 3320-1 (REV 3/99) EPAForm 3320-1 (REV Previous 3199) may editions be used Page 1 of 1

ML11160A101

Contents

Text

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

.25/E

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ML11160A101

Text

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

.25/E

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