ML11160A101
| ML11160A101 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 06/08/2011 |
| From: | Skaggs M Tennessee Valley Authority |
| To: | Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML11160A101 (109) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 8, 2011 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MAY 2011 Enclosed is the May 2011 Discharge Monitoring Report for Sequoyah Nuclear Plant. Sample collection at the Diffuser Pond Inlet of the Yard Pond effluent due to a transformer oil spill that reached the Yard Drainage Pond has been discontinued as of 5/14/2011. Samples collected from 9/23/2010 through 5/14/2011 have all yielded results below detection limits for oil and grease. If you have any questions or need additional information, please contact Brad Love at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, Michael D. Skaggs Site Vice President Sequoyah Nuclear Plant Enclosures cc (Enclosures):
Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE OPS-ELN-SN)
SODDY - DAISY. TN 37384 FacilitY VA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY-A1TN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TNO026450 J10G PERMIT NUMBER DISCHARGE NUMBER-F MONITORINr PERIOD vrYI:AR I MO I nAY, I IYE RI M Froml 11 o05 1 01 1 Tol 11 1 05 311 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0C F - FINAL DIFFUSER DISCHARGE EFFLUENT
- NO DISCHARGE E
NOTE: Read instructions before comoletino this form.
104 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 35.7 04 0
31 / 31 RCORDR CENTIGRADE MEASUREMENT 00010 1
0 RERMET Req. Mon.
DEG. C.
CONTI CALCTD EFFLUENT GROSS DAILY MAX NUOUS I
TEMPERATURE, WATER DEG.
SAMPLE 25.9 04 0
31 / 31 MODELD CENTIGRADE MEASUREMENT 00010 Z
0 PERMIT 30.5 DEG. C.
CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAIL.NUOUS TEMP. DIFF. BETWEEN SAMP. &
SAMPLE 2
04 0
31 / 31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1
S PERMIT 3
DEG. C.
CONTI CALCTD REQUIREMENT EFFLUENT GROSS I.
DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1644 03***
0 31 / 31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 PERMIT Req. Mon.
MGD
-****CONTI RCORDR EFFLUENT GROSS REQUIREMENT-DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE 0.023 0.046 19 0
21 /31 GRAB MEASUREMENT 50060 1
0 JPERMIT
.1
.01 MGIL FIVE PER CALCTD EFFLUENT GROSS MOAVG DAILYMAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0
62 0
31 / 31 CALCTD CHANGE MEASUREMENT 82234 1
0 PERMIT.
DEG CONTI CALCTD EFFLUENT GROSS REQUIREMENT AIC/HR
-2DECNUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE
-irection or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE TYPED________
OR PRINTED including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTEDOFIEORATOIEAEN ARA NMR YAR O
DY CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1. Towerbrom 960 2. Floguard MS6236 (max. calc. conc. was 0.1lmg/L--Iimit 0.2mg/L) 3. Spectrus CT1300 (max. calc. conc. was 0.039mg/L--limit 0.050mg/L)
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
Mean# of Water SUB NOTES: %
Mean # of WaterWaeSU Sample Date ZM/m3
% Settlers Temp. (°C)
Sample Date Asiatic Temp. (°C)
LOCATION LOCATION Gravid Asiatic COLLECTED BY Clams/m3 Clam 12/07/2010 12/14/2010 12/22/2010 12/29/2010 01/04/2011 01/11/2011 01/18/2011 01/25/2011 02/02/2011
.02/08/2011 02/15/2011 02/22/2011 03/01/2011 03/08/2011 03/16/2011 03/23/2011 03/30/2011 04/06/2011 04/08/2011 04/20/2011 May 2011 6
0 0
0 0
0 0
0 0
0 0
20 0
0 22 0
0 18 45 21 100 0
0 0
0 0
0 0
0 0
0 100 0
0 0
0 0
100 100 100 23 10 10.5 26 13 22 9.5 23 10 9
23 10 11 11 11 11 12 15 15.5 16 12/07/2010 12/14/2010 12/22/2010 12/29/2010 01/04/2010 01/11/2010 01/18/2010 01/25/2011 02/02/2011 02/08/2011 02/15/2011 02/22/2011 03/01/2011 03/08/2011 03/16/2011 03/23/2011 03/30/2011 04/06/2011 04/08/2011 04/20/2011 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 23 10 10.5 26 13 22 9.5 23 10 9
23 10 11 11 11 11 12 15 15.5 16 1-25-545 1-25-545 1-ISV-24-1234 1-25-545 1-25-545 1-25-545 1-ISV-24-1234 1-25-545 1-25-545 1-25-545 1-25-545 1-25-545 1-ISV-24-1236 1-ISV-24-1236 1-ISV-24-1234 1-ISV-24-1234 1-1 5v-24-1236 1-ISV-24-1234 1-1SV-24-1236 1-1SV-24-1236 PB RS WE WDT PB RS CR WDT PB MJW MLW PB PB WE MLW MLW MLW HMW WAW/PB PB No Samples Collected
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O.BO2000 (INTEROQFICE OPS-5N-SQN)
SODDY - DAISY, TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HIAMILTN_ COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) 0MB No. 2040-0004 TN026450 10T F-FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 MONITORING PFRIOD I
EFFLUENT r YoARm MOA DAY IT From[ 11 1 05 1 01 1 TOI F I osI 3 NO DISCHARGE E]---
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE I
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
1/180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1
0 PERMIT 43.2 PERCENT SEMI COMPOS EFFLUENT GROSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT
.REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael 0. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site sident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I
including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED ORPRINTED I
COD COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Toxicity sampling began on May 1 and ended on May 6. The toxicity report is included.
EPA Form 3320-1 (REV 3/99)
Previous editions maybe used Page 1 of 1
June 3* 2011 Bradley M. Love, OPS 5N-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING. NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS. MAY, 2011 Per your request, I have also submitted an electronic copy of the subject report. Outfall 101 samples collected May 01-06, 2011, showed no toxic effects to fathead minnows or daphnids.
The resulting IC2 5 values for both species were> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since lish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV-treated samples.
Please call me at your convenience if you have any questions or comments following your review of the report.
Donald W. Sno grass Manager (Acting),
Environmental Engineering Field Team-Muscle Shoals CTR 2L - M Attachment Cc (Attachment):
R.M. Sherrard, PSC IX - C (Electronic copy)
EDMS, MPB 1E - M (Electronic copy)
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: June 03, 2011
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1579
- 7.
1Q10: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: May 01 - 06, 2011
- 10. Average Flow on Days Sampled (MGD): 1635.7, 1623.12, 1618.58
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
- 12. Test Dates: May 03 - 10, 2011
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: IC 5 = 43.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: I__.> 100%
Ceriodaphnia dubia: IC2 > 100%
UV treated Outfall 101: Pimephales promelas: IC25 > 100%
Page 1 of 100
- 18. Facility
Contact:
Brad Love Phone#: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #:. (828) 350-9364
- 21. TVA
Contact:
Donald W. Snodgrass Phone #: (256) 386-2787
- 22. Notes: Outfall 101 samples collected May 01 - 06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >
100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 2 of 100
.METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3.
Sample Information:
Date Date Date (MM-DD-YY)
(MM-DD-YY)
Arrival Initial (MM-DD-YY)
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 05-01-11 0800 to 05-03-11 1415 101 05-02-11 0700 05-02-11 1512 0.7,0.8t
<0.10 05-04-11 1318 05-01-11 0845 to 05-03-11 1415 Intake 05-02-11 0745 05-02-11 1512 1.2
<0.10 05-02-11 0745 05-04-11 1318 101 05-03-11 0800 to 05-04-11 1437 0.7, 0. 9t
<0.10 05-05-11 1319 05-04-11 0700 05-06-11 1317 05-03-11 0830to 05-05-11 1319 Intake 05-04-11 0730 05-04-11 1437 0.8
<0.10 05-04-11 0730 05-06-11 1317 05-05-11 0800 to 0.9, 0.9, 05-07-11 1319 101 05-06-11 0700 05-06-11 1515 1.5 tt
<0.10 05-08-11 1322 05-09-11 1316 05-05-11 0830 to 05-07-11 1319 Intake 05-05-11 0730 05-06-11 1515 0.9
<0.10 05-08-11 1322 05-06-11 0730 05-09-11 1316
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
tSamples were collected in two 2.5 gallon cubitainers and one 5 gallon cubitainer. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 + 1.0°C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page 3 of 100
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1. Source:
- 2. Age:
Test Method Summary:
- 1. Test Conditions:
- 2. Test Duration:
- 3. Control / Dilution Water:
- 4. Number of Replicates:
- 5. Organisms per Replicate:
- 6. Test Initiation: (Date/Tim Outfall 10 UV Treated Outfall 10
- 7. Test Termination: (Date/]
Outfall 101 UV Treated Outfall 10
- 8. Test Temperature: Outfal Test Temperature: UV-Tr Aquatox, Inc.
21.75 - 22.25 hours2.893519e-4 days <br />0.00694 hours <br />4.133598e-5 weeks <br />9.5125e-6 months <br /> old Static, Renewal 7 days Moderately Hard Synthetic In-house Cultures
< 24-hours old Static, Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 10 1
05-03-11 1415 ET 05-03-11 1345 ET 05-03-11 1315 ET 1
05-10-11 1325 ET 05-10-11 1224 1
05-10-11 1342 ET 1101:
Mean = 24.7°C Mean = 25.0°C (24.2 - 25.20C)
(24.7 - 25.20C)
- eated Outfall 101:
Mean = 24.80C (24.2 - 25.2 0C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page 4 of 100
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 03 - 10, 2011 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(%Effluent) 1 J 2 3
4
[
5 6
7 Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 98 98 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 98 95 93 Mean Dry Weight (mg)
Test Solutions (replicate number)
(%Effluent) 1 2
3 4
Mean Control 0.704 0.756 0.799 0.819 0.770 10.8%
0.849 0.889 0.898 0.768 0.851 21.6%
0.659 0.886 0.913 0.879 0.834 43.2%
0.761 0.844 0.841 0.853 0.825 86.4%
0.845 0.805 0.716 0.797 0.791 100.0%
0.801 0.696 0.863 0.800 0.790 Intake 0.893 0.794 0.774 0.660 0.780 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = IO0/LC50: TUc = 100/IC25 Page 5 of 100
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 03 - 10, 2011 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions
-1 F2 3
(% Effluent)
Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 100 100 100 86.4%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Test Soluetis Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 1 8 9
o 10 Mean Control 28 28 31 31 31 31 30 29 29 29 29.7 10.8%
33 30 34 32 27 33 30 28 30 32 30.9 21.6%
33 32 31 36 29 32 31 31 31 33 31.9 43.2%
36 32 34 30 35 36 35 34 32 34 33.8 86.4%
34 35 33 35 36 37 36 35 33 35 34.9 100.0%
38 36 36 37 35 38 36 36 36 36 36.4 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.2%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50: TUc = 100/IC25 Page 6 of 100
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 03 - 10, 2011 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 1
(% Effluent) 1
_2 3__56_
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Tet Soluetis Data (replicate number)
(%Effluent) 1 2
3 4
5 6 17 8 19 110 Mean Control 29 26 34 30 29 27 30 32 31 29 29.7 Intake 37 37 35 35 37 35 32 36 38 36 35.8 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC 25 Page 7 of 100
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 03 - 10, 2011 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 [
2
]
3 4
1 5
6 7
Control 100 100 100 100 100 100 100 10.8%
100 100 100 100 100 100 100 21.6%
100 100 100 100 100 100 100 43.2%
100 100 100 100 98 98 98 86.4%
100 100 100 100 98 98 98 100.0%
100 100 98 98 98 98 98 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg)
Test Soluetis (replicate number)
(%Effluent) 1 2
3 4
Mean Control 0.714 0.908 0.853 0.849 0.831 10.8%
0.901 0.813 0.867 0.888 0.867 21.6%
0.896 0.793 0.779 0.829 0.824 43.2%
0.879 0.852 0.847 0.773 0.838 86.4%
0.850 0.770 0.774 0.797 0.798 100.0%
0.816 0.802 0.660 0.840 0.780 Intake 0.948 0.883 0.925 0.799 0.889 IC 2 5 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species F
Date Time Duration Toxicant Results IC25)
Pimephalespromelas May 03 - 10, 2011 1445 7-days KC1 0.78 g/L Ceriodaphnia dubia May 03 - 10, 2011 1255 7-days NaCl 1.07 g/L Page 8 of 100
PHYSICAL/CHEM ICAL SUM MARY Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 03-10, 2011.
Test Sample ID Temperature (C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (tmhos/cm)
(mgfL CaCO3) (mg/L CaCO3) Chlorine (mg/L)
Control 24.8 24.6 7.6 7.7 7.81 7.77 321 59 90 24.7 - 24.9 24.4 - 24.8 7.4 -
7.7 7.5 7.9 7.73 - 7.89 7.68 - 7.86 305 -
333 58 59 89 91 10.8%
24.9 24.6 7.8 7.7 7.85 7.73 299 24.7 -
25.0 24.2 -
24.8 7.5 8.2 7.3 -
8.0 7.80 -
7.93 7.64 - 7.87 283 -
311 24.9 24.6 7.9 7.7 7.85 7.73 282
%)
21.6%
24.7 -
25.0 24.4 -
24.8 7.7 8.1 7.3 8.0 7.79 -
7.93 7.64 - 7.86 272 -
292 Q.
24.9 24.6 7.8 7.7 7.83 7.73 249 43.2%/
24.7 -
25.0 24.5 -
24.8 7.7 8.1 7.4 -
8.1 7.77 -
7.92 7.66 - 7.86 241 -
257 24.9 24.5 7.8 7.7 7.80 7.71 188 86.4%
24.7 -
25.1 24.4 -
24.6 7.7 8.0 7.4 8.1 7.72 -
7.92 7.66 -
7.85 179 -
207 100.0%
25.0 24.5 7.9 7.7 7.79 7.71 163 58 63
< 0.10 24.9 -
25.2 24.3 -
24.7 7.7 8.1 7.4 -
8.0 7.71 -
7.90 7.64 -
7.85 157 -
174 54 61 55 71
<0.10 -
<0.10 Intake 24.9 24.4 7.9 7.7 7.78 7.70 164 59 62
< 0.10 24.7 -
25.1 24.3 -
24.6 7.8 8.1 7.3 -
8.0 7.69 -
7.92 7.64 - 7.87 155 -
178 55 61 55 67
<0.10 -
<0.10 Control 24.9 25.0 7.6 7.7 7.81 7.84 321 59 90 24.8 -
24.9 24.7 -
25.2 7.4 -
7.7 7.4 -
8.0 7.73 -
7.89 7.77 -
7.90 305 -
333 58 59 89 91 10.8%
25.0 25.0 7.8 7.7 7.85 7.82 299 24.9 -
25.0 24.8 -
25.2 7.5 8.2 7.5 8.0 7.80 -
7.93 7.71 7.90 283 -
311 26 25.0 25.0 7.9 7.8 7.85 7.84 282 24.9 -
25.0 24.8 -
25.2 7.7 8.1 7.6 8.0 7.79 -
7.93 7.77 -
7.91 272 -
292 25.0 25.0 7.8 7.8 7.83 7.84 249 43.2%
24.9 -
25.0 24.8 -
25.1 7.7 -
8.1 7.6 -
8.0 7.77 -
7.92 7.76 - 7.90 241 -
257 25.0 25.0 7.8 7.8 7.80 7.84 188 24.9 -
25.1 24.8 - 25.2 7.7 8.0 7.6 -
8.0 7.72 -
7.92 7.74 - 7.93 179 -
207 100.0%
25.1 25.0 7.9 7.8 7.79 7.83 163 58 63
< 0.10 24.9 -
25.2 24.8 -
25.2 7.7 -
8.1 7.5 -
8.0 7.71 -
7.90 7.72 - 7.93 157 -
174 54 61 55 71
<0.10 -
<0.10 Intake 24.9 24.9 7.9 7.8 7.78 7.82 164 59 62
< 0.10 24.9 -
25.1 24.8 - 25,2 7.8 -
8.1 7.6 -
8.1 7.69 -
7.92 7.71 - 7.91 155 -
178 55 61 55 67
<0.10 -
<0.10 Overall temperature (C)
Pimephales promelas Ceriodaphnia dubia Average 24.7 25.0 Minimum 24.2 24.7 Maximum 25.2 25.2 Page 9 of 100
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 03-10, 2011.
Test Sample ID Temperature (C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO3) (mg/L CaCO3) Chlorine (mg/L)
Control 24.9 24.6 7.8 7.7 7.83 7.74 311 59 92 24.8 - 24.9 24.4 - 24.9 7.5 -
8.1 7.5 -
8.0 7.78 - 7.91 7.66 - 7.84 299 -
326 57 60 90 96 10.8%
24.9 24.6 7.8 7.7 7.85 7.75 297 24.9 - 25.0 24.3 - 24.8 7.7 -
8.1 7.3 8.0 7.79 - 7.92 7.66 - 7.85 286 -
310 25.0 24.6 7.8 7.7 7.86 7.74 283 24.9 - 25.1 24.2 - 24.8 7.7 -
8.1 7.3 7.9 7.80 - 7.93 7.66 - 7.86 270 -
293 43.2%
25.0 24.6 7.9 7.6 7.85 7.73 250 24.9 - 25.1 24.5 - 24.7 7.7 -
8.1 7.3 7.9 7.79 - 7.91 7.65 - 7.86 240
-. 256 25.0 24.6 7.9 7.7 7.83 7.71 187 86.4%
24.9 - 25.1 24.5 - 24.7 7.8 -
8.1 7.4 -
8.0 7.75 - 7.90 7.62 - 7.87 178 -
197 100.0%
25.1 24.6 8.0 7.7 7.81 7.69 164 57 69
< 0.10 24.9 - 25.2 24.5 - 24.7 7.9 -
8.1 7.4 -
7.9 7.73 - 7.91 7.62 - 7.86 154 -
178 52 61 63 76
<0.10 -
<0.10 Intake 25.0 24.6 8.0 7.8 7.79 7.70 164 58 69
< 0.10 24.8-25.1 24.4 - 24.7 7.9 -
8.2 7.5 8.0 7.71 - 7.90 7.63 - 7.85 155 -
175 52 63 63 76
<0.10 -
<0.10 Overall temperature (C)
Pimephales promelas Average
.24.8 Minimum 24.2 Maximum 25.2 Page 10 of 100
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected May 01 - 06, 2011, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 11 of 100
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-82 1 -R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 100
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821 -R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaCl.
- 2.
Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 100
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82 l-R-02-013 (October 2002).
- 3.
Standard Methods for the Examination of Water and Wastewater, 2 1 st Edition, 2005.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 100
Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 6, 2011 Date Sddium Towerbrom PCL,-222,7 PCL-401 Ct3-63 Cuprostat-H-Hykpfochk)Fite mg/L iu flgL
~
mg/L mg/i i,,L PF 1:t1'mL L
?:
TRC Plho itiat Copolymer DMAD mg/L
(
Azole 03/12/1998
'0.016W, 3/4 K
03/13/1998
- 0.
5.015 03/14/1998 y-O.01..
03/15/1998 0~t.03()~
03/16/1998
,0.013 03/17/1998
'i 0.0201 03/18/1998
<>,0.018 09/08/1998 0.01 0014 0.005 0
09/09/1998 0.003,,
( 1 31 0.011 09/10/1998 0.0142 0.)00d 0.021 09/11/1998 0.013 0.019 09/12/1998 0001 040.015 09/13/1998 ZO.0 1 0.015 09/14/1998 0.008, 0,0'44 1
0.015 1
02/22/1999
<-O...
0 02/23/1999 *,
- O005l
- 02/24/1999 0.009 02/25/1999 &*
0.0 12 02/26/1999 0.008
- i.
02/27/1999 02/28/1999 1
08/18/1999 0.015 0100W9 0.024 08/19/1999 Ž>!
KWi 0.012 0>..068 0.024 08/20/1999
'<0.023
>0.070 0.024 0.120 08/21/1999 t
0.022
- 0.068 0.024 08/22/1999 0.022 O6 '-x 0.024
-,-*--t-08/23/1999 0.025
(.0068 0.024 9006 08/24/1999, 0.016
- q.
0.016 7
1 0.023 0020.
(
Page 16 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Sod iu Towerbrom PCL-2222 PCL-401 1-36o3 Cuprostat-Hf-13* M Hpochiar-ite:
mg/L m
gL m
I g-IVTC Pbm/
PF i/
sae Copolymer tDMAD mg/L Quat TR, Azole
)1/31/2000
< 0.002 0.026 0.009
)2/01/2000 0.011 0.026 0.028
)2/02/2000 0.028 0.026 0.009
- 0.006
)2/03/2000 0.008 0(0)7.2 0.009
)2/04/2000 0.006 0.027 0.009 00.109
)2/05/2000
< 0.002
- 0.
0.009 K.
)2/06/20001
< 0.002 0,.027 1
0.009
-1[-
)7/26/2000
< 0.0057 0 055 0.019
)7/27/2000 0.019 0055 0.019
)7/28/2000
<0'0080.0088 0505'0 0.018 0:001()4 0.108
<7/28/2000
< 0.0088 0505 0.019 27/30/2000
<0.0076
(). ( )
0.019 27/31/2000
< 0.0152
,0255 0.019 0.006 28/01/2000
< 0.0141
- 0.025 1
0.019 20
.00.
[2/11/2000 0.003 0.020 0
12/12/2000 0.0092 O
0.020
..000.
12/13/2000
< 0.0120 0.06
- 0.020-005 12/14/2000
< 0.0087 01.06 i
0.0210.
12/15/2000 0.0120
()12,
0.020
>000:5 I0,021 12/16/2000
< 0.0036 0
0.020
- O.,05 12/17/2000
< 0.0036 1 0,025 0.020 18/26/2001 0.017 00.021
,0 18/27/2001
<0.0096 0024 0.021 0.005 18/28/2001
<0.0085 0023 -
0.021 0.07 18/29/2001
<0.0094 50.059 0.020
- 0. 011 6 8/30/2001
<0.0123 0.02*
0.021 28/31/2001*
1 <0.005 2 0590 0.020 11/25/2001
<0.0044 i
11/26/2001
<0.0119
().W4 0.02 21/27/2001 0.0137 0.024 0.019
,0.00 112/01'*>?'
}<0.0089 2
0.019 iO'HO 11/29/2001,<,,,a+ *<,,*
0.0132
().(0 24 0.02
!~ n 1/30/2001
< 0.0043 0024 0.02
.2/09/2001
<0.0042 0,
0.02 0.00, 12/10/2001
<0.0042
"='
}
.2/I11/2001 *
'{*
i<0.0104
?ir
,2/12/2001 o,
0.0128 0.02:
04.o.00 1 2/13/2001
<0.0088 0",'024 0.02 i00Y*
Page 17 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Sodium Towerbrom PL-222 PCL-401 CL-1363*
Cuprostat-iH-.130N/
Hypochiorite mg/L mg/
i mg/L mlýI~
PF 111,g1 L
mg"L TRC spnate:
Copolymer DMAMY mg/L Qii"t TRC Azole
)1/02/2002
< 0.0079 0023 0.02 0J6
)1/03/2002
<0.0042 0.014
)1/04/2002 0.0124 2 0024 0.014
- 00
)1/05/2002
<0.0042
)1/06/2002
<0.0042
)1/07/2002
< 0.0089 0.024 0.014 o6i006
)2/24/2002
< 0.004
)2/25/2002
<0 0.004 0023W-)
0.023 2
!\\.
)2/26/2002 "00143 °0.
- 023' 0.023
_;0:007'
)2/27/2002
< 0.0041
- 0023 0.023
)2/28/2002
< 0.0041 0.024 0.008
)3/01/2002
< 0.0041 0.024 1
0.008
)5/05/2002
-'""""* }
)5/06/2002 0080.02 0.014
)5/07/2002 05 0.02 15,
)5/08/2002 0.0 0.019
)5/09/2002 (0.057 0.02 WO-14
)5/10/2002 1
(0.056 0.019
)8/04/2002 "
<0.0058
-018
)8/05/2002
<0.0058 0.053 0.018
-0.025
)8/06/2002 i 0.0092 0530.018
)8/07/2002
<0.0107
().055 0.0719 0.0
)8/08/2002 *
<0.0061 0:055 0.019
)8/09/20021 1 0.0152 5
O054*
0.018 0.008
,0/06/2002
<0.00497 0/07/2002 0.0153 0.054 0.018
.0/08/2002
<0.0092 0.054 0.018 O0007 10/09/2002 0.0124
- i
- 0 053 0.018 0w009 10/10/2002 0.0134
- 0. 0 4 0.018 000 10/11/2002 1
<0.0042 0.. 04 0.018 K
)1/12/2003 4"
<0.0035 01/13/2003
- i.
<0.006 0'025 0.019 0009
)1/14/2003 *
<0.0118
(),
0 26, 0.020
)1/15/2003 @
<0.0063 0.026 0.020 0
)1/16/2003
<0.0034 0026 0.020
)1/17/2003
<0.0034 1
0.026.
0.009
)4/06/2003
<0.0073
)4/07/2003
<0.0189 0.021
)4/08/2003
-<0.0 117 0.02 1
)4/09/2003
<0.0139 0.021 0*016
)4/10/2003
<0.0113 0.021 0018
)4/11/20031
<0.0073 1
0.022 Page 18 of 100
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date
[
SodlSdnl Towerbrom +PCL2-2*"'
PCL-401 A.-. 363 Cuprostat-H,11<30O 1yOC ilt mg/L I'
mI-*,L mg/L m./L PF mg/I IIm,-
gL TRC Posphat*e Copolymer
- DMAF mg/L
. Qua RC.
Azole 06/15/2003 [*
<0.0045
-l-.
06/16/2003
< 0.0037 0.020 0,02 06/17/2003
< 0.0048 0041 0.014
- 0t2, 08/06/2003
< <0.0084 0.041 0.0200 16/10/2003
< 0.0108 0.268 0.024 0 0092 06/20/20034 1 < 0.0048 0.027 1
0.020 9
0,02ý 08/03/2003
<0.0034 5
0*0'"
0.009 02/03/2004
<0.0050
- 1) 0.0209.
+
08/04/2003 0.0<051 0.006k 0.00*92:.009 08/05/2003
<0.0034 1-) 2657 0.020 002:
08/06/2003 0.0105 0
0.0209 0n0n0 05/04/20041.
<0.0123 0
0.020 026.019....
05/05/2003
<0.0104
").0572 2 0.020 05/06/2003
<0.0143 (0.057 0.020 0
-0.02' 10/07/2003
<0.0022
,0058 0.020 0.0
-2 10/08/2003
<0.0106 0.67 0.020 02 10/09/20034
<0.0104 0
0.020 0, 0 10/10/20041 0.0187 0.02 0.019 0.00 02/01/2004 0.0093 0.057 0.009 0
02/02/2004
<0.0034 0(.058 0.009 02/03/2004
<0.0034 0.05-61 6
0.009
-0 02/04/2004 0.0124 0.020 0.009 0.009 07/09/2004
<0.0034 (0.057 0.009 0
02/06/2004
-0.0105 70.020 0.009 H'010+*+
05/04/2004
-li7i
@:!*,7
<0.0123 i0.0,26+
0.019 05/05/2004
+
<0.0144
- 0.026 0.014 (0(9*+NO_
05/06/2004
-++*++++
<0.0146
+,': 0.0......"- '!
0.013
,!?.-'*=.......
05/07/2004
-+
......,N 0.0227 0.:058 0.020 fff-0"),,02 05/08/2004
-0.016
- 0, 0+60+
0.021 N
05/09/2004
<0.0104 (0!J)58 0.020 07/04/2004...
0.0217-
+ +n.+: +7 0.019
,,-+-
07/05/2004
++++++: +++
<0.008
.... 0+n -
ý;
0.020 H.00-07/06/2004 i;:::2 :5:Z*<0.0077 0,0ýý0.020 i~i*'
07/07/2004
":::::0.0252 5<u 6*
0.019 X
07/08/2004 0.0223
ý -
0.019 W~~slo111 07/09/20041 1 0.0182 o.0ý7" 0.020
(.*60)(H Page 19 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Sodium Towerbrom ll 222 PCL-401 CL-163 Cuprostat-PF ýH-i30M Nalco Hypochlirit mg/L 1
mg1,2 mgIL ~
gL mgIL mgLI'L 73551 e
TRC Phlsph*atcý Copolymer m)
I..A1 Azole
- ,*t mg/L zEo/Po
<0.0187 0.00()
0.014--
<0.0192 0NO.04'7, 0.030
<0.0233 0,048 0.016 0,04i
<0.0149 0,.:,47 0.016 (o 1
<0.0149 0i049)iig 0.017 OAO".
~<0.0253 0.017 0.4
<0.0042 0.010 S
<0.0116
.0,28' 0.010 0.007
<0.0080
.i O028 >V 0.010 A..-
0.0199 0.0 0.010
<0.0042 0.,02.8 0.010
- .0 0.0155 0&*
028 0.010
{
0.007 0.0063 0.0043 S0.0103
- 0. 0295 0.0129 0.0184
'-2l~i 0.0109 o.026 0.009 0.0150 0026 0.009 0.0163 o0.00 g->
0.0209 3
002t4 0.009 0.014 0.0200 0.009
- !*i0.0238 OOS 0.018 0.014 i* !*!i*!*0.0104 t
g4:i
- v,*:}
0.0117
-. *i...
~0.0165 0.0256 0.0116
,:~g Page 20 of 100
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 6, 2011 Date Towerbron mg/L TRC PCL-401 mg/L Copolymer Cuprostat-PF mg/L Azole Nalco 73551 mg/L EO/PO 11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006 11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006 05/28/2007 05/29/2007 05/30/2007 05/31/2007 06/01/2007 06/02/2007 12/02/2007 12/03/2007 12/04/2007 12/05/2007 12/06/2007 12/07/2007 04/13/2008 04/14/2008 04/15/2008 04/16/2008 04/17/2008 04/18/2008 10/26/2008 10/27/2008 10/28/2008 10/29/2008 10/30/2008 10/31/2008 0.0055 0.0068 0.0143 0.0068 0.0267 0.0222 0.0188 0.0138 0.0120 0.0288 0.0376 0.0187 0.0084 0.0103 0.0164 0.0305 0.0241 0.0128 0.0238 0.0158 0.0162 0.0175 0.0039 0.0124 0.0229 0.0143 0.0120 0.0149 0.0260 0.0151 0.0172 0.0154 0.0086 0.017 0.017 0.017 0.018 Page 21 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - May 6, 2011 Date Sodium Towerbror PCL-PCL-401 CL-363 Cuprostat H-130M Nalco
ý[)pectrus Hil-iS5M Hypochllori mg/L 2
mg/L mg/L
-PF mg/L mg/L 73551 0T1300 mg/Lst n,L TRC mg/ L Copolymer DMA D Azole 2Quat t
mg/L m4ng/L Quat Phopl EO/PO
>~Quat 02/08/2009 0.0197 0.017 02/09/2009 0.0237
~
x-
~
0.017 02/10/2009 0.0104 0.021 02/11/2009 0.0155 0.017 I
02/12/2009 2
0.0106 0.017
\\
02/13/2009,
05/10/2009 0.0129 05/11/2009 0.0-415 0.0446 05/12/2009
<0.010 0.0396 05/13/2009 0.0049 N
0.0396 05/14/2009
<0.0141 rit-0.039,
-0.0306t-05/15/2009
<0.02160 11/15/2009 0.025 11/16/2009 0.0152 11/17/2009 0.0255 11/18/2009 0.0306 ~
11/19/2009
& ~
0.0204 11/20/2009 S<
0.0093 05/09/2010
~
-~
0.0192 05/10/2010
>~
't 0.0055 05/11/2010 >c' 0.0100 0.039) 05/12/2010 >'aK 0.0171 0.03,9 05/13/2010 0.0041 0.03 05/14/2010 0.0099 0
- 1.0391-MSW 101 mg/L Phosphate Page 22 of 100
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 - May 6, 2011 Date Sdium o
Towerbro*-PC I PCL-401
!CL-363. Cuprostat H-130M Nalco Sp i-it
- H I
50M Dae ~
oiiii;h Towterbro 2 2PCL*
PC/L mm/-.
73551 222loc mg/L jmgL
-PF mg/L n
('Tl309 **mg
.I. I TRC mg/Li Copolymer DMAbD Azole Q*A 7 mg/L 111!]-
Quat I R Ilisph EO/PO t
Qualit
~~< ~7ate7 10/31/2010 11/01/2010 0.0122 11/02/2010 0.0112 11/03/2010 0.0163 11/04/2010 0.0107 11/05/2010; 0.0132-05/01/2011I*
-i-05/02/2011
&7 I
4 05/03/2011 004ý4 05/04/2011 0.0155 05/05/2011 0.0179 004 05/06/2011 0.0089 i-- -
MSW 101 mg/L Phosphate Page 23 of 100
Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 24 of 100
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number: N/A Asheville, NQ Other (specify): Sonic Delivery Facility Sampled: Sequoyah NP 28801 General Comments:
Ray Duncan:
NPDES Number: TN0026450 Phone:
828-350-9364 Ben Mitchell Sara Snyder_
Collected By: RayDuncan, Ben Mtchell, Sara Snyder Fax:
828-350-9368 Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab.
l~eld Identification /
Gab/Comp.
CollectionDate/Time Container Flow g
AL+C'1ýo8 Sample Description Number&
MGD Rain Event?
Laboratory Use Volume (Mark as Appropriate)
C ollected i: !*; : *,ii: i ii!**ii!:* *i:ii*!i-:*
DtTieYes if Yes, No Trace ETS Log Anwial
~By Time:
Appear-Inches Number Temp.
T ance SQN-101-TOX Comp
~,2 (2.5gal) I6Z-7
.I s
y SQN-INTAKE-Comp al Sample Custody - Fill In From Top Down 4
. n a -,.
Relinquished By (Signature):
Date/Time Received By (Signature):
'.$te/Time 05/02/11
__7 E Sonic Delivery /
05/02/11 E..
Sonic Delivery 05/02/11 ETS 05/02/11 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 25 of 100
- E--U--EI--
P*g 1\\
Whole Effluent Toxicity Sample Receipt Log Date Time Received Received Sample Project Sample Sample name aud description State Comments received
-ei-d by froM.
teperature (C) nbe number numbr 05-02-I 1512 JSmner TVACouier 07,0.8 7068 110502.01 TVA-Seuoyah Nuclear Plant - 101 TN 05-02-Il 1512 J.Sumner TVACourier 1.2 7068 11050202 TVA - Sequoyah Nclea Plant-Itake TN Page 26 of 100
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Client Other (specify): Sonic. Delivery General Comments:
Ray Duncan:
Ben Mitchell Sara Snyder:
Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab.
NPDES Number: TN0026450 rC ec By:Ra3 Dnan, Ben N~hla Sh~
d /J1A~0/
Fax:
828-350-9368 a
Fd I dentificati on /
rak/Comp. K'J> 'ollection Date/Time Sample Description Container Number &
Volume Flow MGD Rain Event?
(Mark as Appropriate) r161ALaborato:ry Use~
Date Time I
7emp..1 By,
-TOX Comp I
Relinquished By (Signature):
Date/Time Received B:
4
- 4.
1 I
05/04/11 05/04/11 Er 1
I I
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 27 of 100
-ErS PagelJ-3 Whole Effluent Toxicity Sample Receipt Log Date Tme Received Received Sample Project Sample Sample name and descrption State Comaseant received recelyed by from temperatue. (C) number number 05-04-11 0955 K. Keenan Fed - Ex 1.4 7076 11050401 Duke Energy Corporatlion - Belews Creek SS NC 05-04-11 0955 K. Keenan Fed - Ex 1.3 7077 110504.02 Bladenboro WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 1.2 7078 110504.03 Progress Energy Carolinas - Cape Fear S. E.
NC 05-04-11 0955 K. Keenan Fed - Ex 1.2 7078 110504.04 Prog ress Energy Carolinas - Cape Fear S. E. - Upstream/Intake NC 05-04-11 0955 K. Keenan Fed - E.
1.0 7079 110504.05 City of Gastonia - Dallas WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 2.5 7080 110504.06 United Water - Enfield WWTP NC 05-04-11 0955 K. Keenan Fed - Ex 2.5 7081 110504.07 United Water - Scotland Neck WWTP NC 05-04-I 1 0955 K. Keenan Fed - Ex 1.0 7082 110504.08 Wilson WWTP NC 05-04-I1 0955 K. Keenan Fed - Ex 1.0 7083 110504.09 Elementis NC 05-04-1I 0955 K. Keenan Fed - Ex 1.0 7084 110504.10 United Water - Farmville WWTP NC 05-04-lI 0955 K. Keenan Fed - Ex 2.5 7085 110504.11 Progress Energy Carolinas - Mayo Electric Steam Plant NC 05-04-lI 0955 K. Keenan Fed - Es 1.4 7086 110504.12 Progress Energy Carolinas - Shearon Harris E&E Center NC 05-04-11 0955 K_ Keenan Fed - Ex 0.9 7087 110504.13 Progress Energy Carolinas - Shearon HarrisPlant NC 05-04-Il 0955 K. Keenan Fed - Ex 0.6 7088 110504.14 Carolina Beach WWTP NC 05-04-11 1000 K. Keenan UPS 0.5 7089 110504.15 Nficrobac Environmental - Rockingham WWTP NC 05-04-11 1000 K. Keenan UPS 0.5 7090 110504.16 Microbac Environmental - Roseboro WWTP NC 1
05-04-11 1000 K. Knenan UPS 0.5 7091 110504.17 Microbac Environmental - JP Stevens - Waan WWTP NC 05-04-11 1000 K. Keenan UPS 0.7 7092 110504.18 Morehead City WWTP NC 05 II 1000 K. Keenan UPS 06 7093 110504.19 vde County - Enmellhad WTP NC UPS shipping error.
05-04-11 1226 K. Keenan Dash Courier 05 7094 110504.20 OWASA - Mason Farms WWTP NC 05-04-11 1437 J, Sumner TVA Coarier 0.7/0.9 7068 110504.21 TVA - Sequovah Nuclear Plant - 101 TN 05-04-11 1437 J. Sumner TVA Courier 0.8 7068 110504.22 TVA - Sequoyah Nuclear Plant - Inlake TN Page 28 of 100 SOP G4 - Exhibit 04.2, revision 06-29-09
-I --.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): (Snc Delivery Facility Sampled: Sequoyah NP 28801 General Comments:
Ray Duncan:
NPDES Number: TN0026450 Phone:
828-350-9364 Ben Mitchell Sara Snyder:
Collected By: Ray Duncan, Ben Mitc
, Sa n der *
/
Fax:
828-350-9368 Metals samples filtered and stored. Samples remained on ice through out sampling and transport to lab.
fteld Identification/
/Grab/Comp.
Collection Date/Time Container Flow j..
0' Sample Description Number &
MGD Rain Event?
Laboratory Use Volume (Mark as Appropriate)
L o
Collected i*
- , :i:: ¢ i ::,!*:
Date Time Yes if Yes, No Trace ETS Log
-Arrival By Time Inches Number Temp.;
an*e SSQN-101-TOX Comp eWl1
'l/t-r1*1 01160 2 (5gal)
'0 Qr-,lii
°qiO*
SQN-INTAKE-Comp os/
05
-1i.
OdI a3o 1 (2.5 gal)
NA7"-
T O X 0 6 010 t o a o at! 1.6.".
- ... -.:... - * ?..
_______________Sample Custody - Fill In From Top Down kM Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Ti:
05/06/11 C/
Sonic Delivery /
05/06/1 1 4-Sonic De ery 05/06/11 ETS 05/06/1l t~
.S>.rn Er 555Er Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that.the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 29 of 100
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/
- ETS Page Whole Effluent Toxicity Sample Receipt Log Date Time Received Received Sample Project Sample Sample name and description State Comments received received b
from temperatore CC) number number 05-06-It 0946 K. Keenan UPS 3.3 7089 110506.01 Microbac Environmental - Rockingham WWTP NC 05-06-11 0946 K. Keenan UPS 3.3 7090 110506.02 Microbaw Environmental - Roseboro WWTP NC 05-06-11 0946 K. Keenan UPS 3.3 7091 110506.03 Miccobaw.Environmental - Westoint - Wagram WWTP NC 05-06-I I 1000 K. Keenan Fed - Ex 1.9 7076 110506.04 'Duke Energy Carolinas - Belews Creek Steam Station NC 05-06-11 1000 K. Keenan Fed - Ex 2.3 7077 110506.05 Bladenboro WWW'P NC 05-06-11 1000 K. Keenan Fed - Ex 2.5 7078 110506.06 Progress Energy Carolinas - Cape Fear S.E.
NC 05-06-I1 1000 K. Keenan Fed - Ex 2.5 7078 110506.07 ProgressEner Carolinas -Cape Fear S.E. -UpsueamvIntake NC 05-06-11 1000 K. Keenan Fed - Ex 2.7 7079 110506.08 City ofGastonia -Dallas WWTP NC 05-06-11 1000 K. Keenan Fed - Ex 2.0 7080 110506.09 United Water - Enfield WWTP NC 05-06-11 1000 K. Keenan Fed-Ex 2.0 7081 110506.10 United Water - Scotland Neck WWTP NC 0i-06-lI 1000 K. Keenan Fed - Ex 0.8 7082 110506.11 Wilson WWTP NC 05-06-11 1000 K. Keenan Fed - E.
18 7083 110506.12 Elernentis NC 05-06-11 1000 K. Keenan Fed - E.
0.9 7084 110506.13 UnitedWater-FarmvilleWWTP NC 05-06-11 1232 K Keenan Dash Courier 1.3 7094 110506.14
!OWASA - Mason Farm-s WWTP NC 05-06-11 1515 J. Sumner TVA Courier 0.9,0.9,1.5 7068 110506.15 TVA -Se oyahNuclcar Plant-101 TN 05-06-11 1515 J. Sumner TVA Courier 0.9 7068 110506.16 TVA - Sequoyah Nuclear Plant - Intake T
_N Page 30 of 100 SOP G4 - Exhibit G4.2, revision 06-29-09
U
.. ~EnvIronmentaITest~ing Solutions, Inc.
Page 1 of 6 I
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Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #:
210206W1 0 UWO Project #:
-1o6&
County: Mf t100rC.'T0 Outfall: 101 Dilution prep aration information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
- 22. -T
- ,bt -%Otzi Randomizing template:
4e-uL_,U,)
Date and times organisms oO*
L..
1%,6 Incubator number and were born between:
shelf location:
X__._
Organism source:
ATW &*,Tt. A ?J0*.at-I I Artemia CHM number:
C4II H i Drying information for weight determination:
Transfer bowl information:
pH =
1.-16 S.U.
Date / Time in oven:
Ot.o-t% 1410 Temperature =
.'&.L. °C Initial oven temperature:
60 *(-
Average transfer volume:
Date / Time out of oven:
-qtcx
- 0. I' t.,Final oven temperature:
liD't_.
Total drying time:
"i-IA0 Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 osl't-lk
- 4.
t4t5 tIAisL
,OI
/lOz.0tQ..
Os-O-II A*
os,-_
ocka
%so t Av
-41*-
o-o.oI tiosoL.rL s.n--A 2
_m-,
i3)qSaq. 2.%
- l0SO4, 2
- -.t4,-l 3
6S.01.*,1 06o% )d
%Soo II 0g. 2(
II0So.
Io.2 1 OS,-q is CS1 I 1o 0 01 0 0 kL
' al~l 11..
tl s a. is lIo s o f. 1 6 A ' S -I 6
os-CA I I no&0 I -
11 wS6(,. is It 0o6. I (
os-os-tl I
I I
is Lis A
--7 Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
- 07.
< 20%
7-day LC50
(
!7.
Average weight per initial larvae:
0.-110
=
NOEC (OO.
Average weight per surviving larvae:
0.1 1 0.25mg/larvae LOEC
- IMI, ChV 1
-2, 't At, 1.
IC25 I__DOI__a Page 31 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
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Page 2 of 6 EnvironmentafTestfng Soiutlons, Inc.
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date:
OS-*- 1\\
Survival and Growth Data I
I I
Day CONTROL 10.8%
21.6%
A B
C D
E F
G H
I I J K
L 0
t)_I o
10./()
1 0/0 t0
/0 /0I
) /0(
1 1
/0 (0D (0
10 It (0
10 0 (1 16 -Io 2
I0 (b
(0 to3 M t If 0t to 10 to Io 2
/1D I0 U
3 11(
10 10 (0
/t /0 /0
/0 5
(C t to to I*
to.1 10/
to*:
to A - Pan weight (mg)
Tray color code::
L.'A't &Lt, Analyst:
Ai WC 14.9(,
1 iIi 1S.6' t
msi 1L-L.
o%5
,t-b isit. 4 A D a te :
C A_. 0 4.. itI B = Pan + Larvae weight (mg)
Analyst:
ffi.L Date:
o0514-*
1, t.,.
to.I0 Z.11 Zit AvS.'J',
C = Larvae weight (mg) = B - A Hand calculated.
"l I
iO 8 eA.
8.& q
.'.8 V,
(.
.6o
- .*b A
.1 '
Analyst:
I I
I I
I Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
0~
I. b' b
o Average weight per Percent reduction initial number of from control (%)
0.11 larvae (nmg).I i -
I -
I -
C -
i -
S -
- lb. '".0
- o. & S4 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 32 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
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I Page 3of 6 IFTS Envifrnmental Testing Solutions, Inc.
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date:
CS. a".-Il Survival and Growth Data Day 43.2%
86.4%
100%
M N
0 P
Q R
S T
U V
W X
0 t 0 to I
o lo0/o o
o
'0 10 to to1010 t o to 16
/0 to (0 /0 (0
1t
/0
/0
./0 2
16 10 10 to
/10 to t(
to D0 (0
I0 tQ 3
/0 I0 to t0 o t(
10
/0
/0 (0
(0
/0 6
10 10 (0
10 to1 t10 to to 0 t0 to to 5
10 0
1O (O
/0 to t
10
/0 to I (0 (0
/0 6
( 10 to to 10
/(O to I o
I (.
to to to 7
1 b I to 10 I to (0
/0 tf 10 to I()
A Pan weight (mg)
Tray color code::
Livm St.L, Analyst:
kkmu.
M*.54 l.qi, 11513 1.1.4 l3-.?
5A) 15.Da*
IZr.
'73.
.('n 13.44 I
m.1*,1 t Date:
05____A_.11 B = Pan + Larvae weight (mg)
Analyst:
M 4.--..
~4..
e.. M Z--. (. ?..
v.A.4
.O L
,,.9 Date:
VA zi4.U u(4UttML l-l A010.0U11M C = Larvae weight (mg) = B - A Hand calculated.
9-A&44J~~
516 1-4C)S a-U
- 1. 1.111.
1.
Analyst:
4.*
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
\\0 A.
¶
,O.
Analyst:
_ A--
V 1"
0, O"
('
0" Average weight per Percent reduction initial number of from control(%)
0.L2.
-1.27.
3."'
-- 2. 9, 7
.1.
-".1 "i, larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 33 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
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I Page4of 6 Environmental Testing Solutions, Inc.
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date:
OS-C.
\\
Survival and Growth Data Day I
I 0
1 2
3 4
5 6
7 100% Intake Y
Z AA BB
/L) (0
- 10.
1 D
/0
/0
/0 1o
/0
/0 to10 (0D
- 10. 1o D 1 1o tO I) to Ic:1)10 tO
/0
- 10)
IL I,,
Ql t,.*
o.'
-\\,.
I A = Pan weight (mg)
Tray color code::
LaAv SLa Analyst:
ItA P Date:
t ok B = Pan + Larvae weight (mg)
Analyst:
104 Date:
o5. 14-_
C = Larvae weight (mg) = B - A Hand calculated.
Analyst:
I Weight per initial number of la rvae (mg)
=C /Initial number of larvae Hand calculated.
Analyst:
Average weight per Percent reduction initial number of from control (%)
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 34 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
.. 1- -. 1. ý 0
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 PIimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Prioiject number:
7068 Not forComplin-c Asssessment, Internal LaboratoryQC Cmontrll. p(%)
Repliate loideet mbera Flaul mueaer tln-v A= P=
Pawnweiht(ng B=lPaoL rvae Laa ewelght(mg) WelghtlSar,,iv Mean w Me ightdIt Co.titeon..Ind.
Weighti
.tt ulnfier Bluennsarviv.1 -wdea..wdghtlaiCtla o
Coaefiatd Peoefrtrednitd=ntres I-weight uin (5aJ
=A-B tsnsen (
S..
Srvbdtau nmbeero W
Zee "olase (rat)
(%)
iunberi larvae variation t4r-motrol (%)
larva (mrg) w
.'wt(%)
(rng) snsbea A
10 10 14.85 2189 7.04 0.704 0.704 Control B
10 10 14.06 21.62 7.56 0.756 0770 6.6 0756 100.0 0.770 6.6 Not applicable C
10 10 14.34 22.33 7.99 0.799 0
0.799 D
10 10 13.19 21.38 8.19 0.819 0.819 E
10 10 13.36 21.85 8.49 0.849 0.849 10.8%
F 10 10 13.89 22.78 8.89 0.889 7.0 0.889 G
10 10 13.81 22.79 8.98 0.898 0.898 H
10 10 12.55 20.23 7.68 0.768 0.768 1
1 10 9
14.01 20.60 6.59 0.732 0.659 21.6%
1 10 10 12.85 21.71 8.86 0.886 0.853 9.6 0.886 97.5 0M4 14.1
-8.4 K
10 10 13.91 23.04 9.13 0.913 0.913 L
10 10 14.49 23.28 8.79 0.879 0.879 M
10" 10 13.54 21.15 7.61 0.761 0.761 43.2%
N 10 10 13.92 22.36 8.44 0.844 0825 5.2 0.844 100.0 0.825 S.2
-7.2 0
10 10 13.23 21.64 8.41 0.841_
0.841 P
10 10 1t.74 20.27 8.53 0.853 0.853 Q
10 10 13.83 22.28 8.45 0.845 0.845 86.4%
R 10 10 13.79 21.84 8.05 0.805 0791 6.8 0.805100.0 0.791
- 6.
-2.
S 10 I0 15.00 22.16 7.16 0.716 0.716 T
10 10 14.76 22.73 7.97 0.797 0.797 1
U 10 10 13.63 21.64 8.01 0.801 0.801 100%
V 10 10 13.44 20.40 6.96 0.696 0,790 8.8 0.696 1O0.0 0.790 8.8
-2.7 W
10 10 13.49 22.12 8.63 0.863 0.863 X
10 10 12.97 20.97 8.00 0.800 0.800 S
1010 14.35 23.28 8.93 0.893 0.893 00% Intake 110 60 13.38 21.32 7.94 0.794 0851 9.4 0.794 92.5 0.780 12.2 AA 10 10 14.45 22.19 7.74 0.774 0.774 BB 10 7
12.24 18.84 6.60 0.943 0.660 Outrfan 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
0.1196 15.5 0.1052 13.7 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimutt percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) ý 12%.
Upper PMSD bound detenuined by USEPA (90th percentile) - 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET tlterlaboratoty Variability Study (USEPA, 2 0 01a; USEPA, 2001 b).
USEPA. 2001a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-tct-m Chronic and Acute Whole Effluent Toxicity Test Methods, Volutmes I and 2-Appendix. EPA-82t-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
Page 35 of 100 Fi: sqn101_O50311datAldx Entered by: J. Sumner Reviewed by:
" S 0
Environmental Testing Solution s, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101
.Non-treated May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/3/2011 Test ID:
PpFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments.:
Conc-%
1 2
3 4
D-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000, Intake 0.8930 0.7940 0.7740 0.6600 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.7695 1.0000 0.7695 0.7040 0.8190 6.623 4
0.8199 1.0000 10.8 0.8510 1.1059 0.8510 0.7680 0.8980 6.967 4
-1.643 2.410 0.1196 0.8199 1.0000 21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4
-1.305 2.410 0.1196 0.8199 1.0000 43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4
-1.114 2.410 0.1196 0.8199 1.0000 86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4
-0.428 2.410 0.1196 0.7908 0.9645 100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4
-0.413 2.410 0.1196 0.7900 0.9636 Intake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4
Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88409168 0.884
-1.217221232 1.2704004 Bartlett's Test indicates equal variances (p = 0.56) 3.9158771 15.0863171 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100
>100 1
0.11955186 0.15536303 0.00391794 0.00492163 0.566574454 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point SD 95% CL(Exp)
Skew IC05 ICI0 IC 15
>100
>100
>100 IC20
>100 lIC25
>100 1C40
>100 1C50
>100 Page 36 of 100 File: sqnl0l_05031 ldata.xlsx Entered by: J. Sumner Reviewed by:
I.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated "TS 1
Environmental Testing Solutions, Inc.
May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/3/2011 Test ID:
PpFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 10 1 - Intake End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Conc-%
1 2
3 4
D-Control 0.7040 0.7560 0.7990 0.8190 10.8 0.8490 0.8890 0.8980 0.7680 21.6 0.6590 0.8860 0.9130 0.8790 43.2 0.7610 0.8440 0.8410 0.8530 86.4 0.8450 0.8050 0.7160 0.7970 100 0.8010 0.6960 0.8630 0.8000 Intake 0.8930 0.7940 0.7740 0.6600 Transform: Untransformed I-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 0.7695 1.0000 0.7695 0.7040 0.8190 6.623 4
10.8 0.8510 1.1059 0.8510 0.7680 0.8980 6.967 4
21.6 0.8343 1.0841 0.8343 0.6590 0.9130 14.114 4
43.2 0.8248 1.0718 0.8248 0.7610 0.8530 5.190 4
86.4 0.7908 1.0276 0.7908 0.7160 0.8450 6.839 4
100 0.7900 1.0266 0.7900 0.6960 0.8630 8.766 4
Intake 0.7803 1.0140 0.7803 0.6600 0.8930 12.249 4
-0.199 1.943 0.1052 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98316872 0.749
-0.2349007 0.4704176 F-Test indicates equal variances (p = 0.33) 3.51607203 47.4683456 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.10523383 0.13675612 0.00023113 0.00586562 0.84920698 1,6 Treatments vs D-Control Page 37 of 100 File: sqnl0l_050311 data.xlsx Entered by: J. Sumner Reviewed by:_
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011
]Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 0
Daily Chemical Analyses Environmental Testing Solutions, Inc.
Project number:
7068 Concentration Parameter Da 0 Day I Day 2
.Day 3
Day 4 1
Day 5 Dav 6 Initial Final Initial IFinal Initial IFinal Initial IFinal Initial IFinal IInitial IFinal Initial IFinal PH (SU) 7.84.
7.80 7.781 7.77 7.891 73781 7.811 T777 7.801 7.86 7.801 7T681 7.73 7.701 DO (mx/L) 7.4.
7.8 7.51 7.6 7.51 7.51 7.61 7.7 7.71 7.91 7.71 7.61 7.61 7.91 Conductivity (lunhos/cin) 333..323534 0
3 2
Control Aikalinity (mgIL CaCO3) 5 95 Hardness (mgIL CaCO3) 9 99
_________Temperature C
24.9 24.8248
- 4.
24.8 24.71 24.9 24.5'47
- 2.
24.71 24.71 24.8
.2.
PH (SU) 7.85 7
7.80 77 7.88 7.671 7.86 775
.82 78 7.93 7.64 7.80 76 DO (
/L) 7
- 7.
7 7.7
- 7.
7.5 7.61 8.2 7.8778 7.9 7.3 7.87.
10.8%
Conductivity (uimhos/cm) 310 311 283 28.8 29.0 307 303
________Temperature
('C) 24.9 248 24.9 242 24.94 N24.6 25.0 247.48 24.5247 24.6 24.8 2.
oH (SU)78
- 7.
75M 7.79 77 7.87 7.66 7.85 776
.84 786.93 7.64 7.82 76 DO (gL) 7.7 7.7 7.7 7.5 7.9
- 7.
8.1 7
.8 78 9
8.1 7.3 7.91.
8.
21.6%
Condiuctivity (Iunhos/cm) 292 291 272 275 275 284 28
________Temperature (C) 24.9 24.6 25.0
- 2.
24.9 244 25.0 24A7 24A8 244.471 2
24.8
- 24.
7 oH( SU) 777 77.77 7.70 7.86 7.661 7.84 777
.84 7.86 7.921 7.68 7.81 76 DO (mg/L) 7.7 7.7 7.7 74 7.8 75 878 7.8 7.9 8.1 7.6 7.88.
43.2%
Conductivity (jumhos/cm) 253 257 241 242 24325 253
_______Temperature (1C) 25.0 24.5 25.0 24.6 24.9 246 25.01 24.6 24.8 245 24.7 24.8 248 24.6 PH (SU) 7.72 7.67 7.73 7.68 7.80 76 7.82 7.75 7.82 785.92 7.66 7.79 7.69 DO mI FL) 7.8 7.7 7.7 7.4 7.8 75 8.0 7.8 7.8 798.0 7.5 7.8 8.1 86.4%
Conductivity (junhos/em) 179 183 185 187
- 11.
207 8
_______Temperature (T) 25.0 24.6 25.0 246 24.9 24.6 25.I 24.4 24.8 24.4 24.7 24.5 24.9 24.6 pH (SU) 7.711 7.681 7.72 7.67 7.761 7.661 7.81 7.74 7.811 7.851 7.90 7.64 7.79 7.71 DO (mg/L)
.7.81 7T81 7.81 7.41 8.01 7.51 8.01
- 7.
7.81 8.01 8.1 7.41 7.7 7.8 Conductivity (pmhos/cm) 1571918.5 166 14417 100%
Alkalinity (mgIL CaCO3) 5 96 Hardness (mgIL CaCO3) 5 37 Total Residual Chlorine (mg/L)
<01<.0 Temperature ('C) 25.01 24.51 25.01 24.31 25.01 24.51 25.21 24.4 25.01 24.31 24.9 24.51 24.91 24.71 PH (SU) 7.691 7.681 7.721 7ý.64 7.751 7.651 7.81 7.73
-7.821 7.87 7.921 7.661 7.781 7.701 Do (mX/L) 7.81 7.81 7.81 7.3 8.01 7.51 8..0
- 7.
7.91 8.01 8.11 7.61 7.81 7.9 Conductivity (Puihos/cm) 15 5
5 59101812 100%o/ intake Alkalinity (mgIL CaCO3) 5 16 Hardness (mg/L CaCO3) 5 36 Total Residual Chlorine (gL
- .0<.0'01 Tem rature CC Ei 25.01 24.51 25.11 24.3 24.91 24.51 24.9 24.4 24.71 24.3]
24.8[
24.41 24.91024.6 Page 38 of 100 File: sqn101 050311 chem.xls Entered by: S. E ans Reviewed by:
IldnIc Page 5 of 6 I
I I
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101. Non-treated Daily Chemistry:
Date:
os0*".%N\\
I Day I
Analyst 0
I 1 -4 Concentration IParameter DO (miz/U Conductivity (umhons/cm)
CONTROL Non-treated
.1-Alkalinity (ma CaCO,/L) 333.tIL
.R
-'tA 4-Hardness (mg CaCO,/L)
I I
I I
I I
Temnerature (°C) 1PH1 (S.U.)
c 10.8%
Conductivity 1963 (Trnehos/cm)
I__ Temperature (0CQ I
di
~
L'4 I
_V.
I I
IA 1
pH (S.U.)
DO (mg/L) l 21.6%
Conductivity (jimhos/cm)
(19r Temperature (°C) oH (S.U.)
DO (melL) 43.2%
Conductivity (g mos/cm)
I 5
__M___
___K.
S~
372.
1,+
s'.
2'A TemI erature ("C) pH (S.U.)
DO (ma/U 86.4%
Conductivity UI_
I-A
-j.'j
.39 t.31 Temnerature (°C)
I
~
MA j~lmlml*_.
pH (SU.)
/4'-L1 4 -*~~-~-----I, DO (mg/L) 100%
Conductivity 161 (ghscm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L) 55 TR chlorine (mg/L) 40.10 Temperature (°C) 1-S.0 S
S j5~
oH (S.U.)
1,Ici pH______
(S.U.
DO (mLY_/*
100%
Intake Conductivity (gmhos/cm)
/15 Alkalinity (mg CaC0 3/L) 55 Hardness 5
(mg CaCO3/L)
TR chlorine (mglL) 0*1 Te....erat....
(0 -"I
~.LJ II
-1*
LZI Teprtr
('Q I
Initial 1ýiia Initial II Fi~nal I nitial Fi nal Page 39 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09 tU AMAýNtftC
I Envion m nt a Tesing Slutlons, Inc.
Page 6 of 6 Species: Pimephales promelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. Non-treated Date:
ln.I I
Day Analyst 4
I 5
I Concen-Parameter tration I
pH (S.U.)
DO (ma/L)
L CONTROL Non-treated Conductivity (iimhos/cm) j Alkalinity I
(mg CaC0 3/L)
HardnessI (mg CaC0 3/L)
.Vol.
Th1 l4
-332, 13A.
- 2-2.1 Temperature (MC)
S pH (S.U.)
- t'.IN II.
1-4 I
B1, II S-+- I "-4m II I
04 1 7.0 11-DO (ma/L) 10.8%
Conductivity (gmnhos/cm)
AA 011'
'3 03
-L-A. I 11A.Lý
'IM D
_1ý4
-L Temperature (0C)
S S
pH (S.U.)
DO (mg/L) 21.6%
Conductivity
[
(iimhos/cm)
ZTL LLSJL 71S.0 2
Temperature (°C) p p
DH (S.U.)
II 1,4 I '1-4 II J.6 1 7-q.52_
II "1.A I 7Pd (l,".
I-~~~~~
1-----*4
~
DO (mR/L) 43.2%
Conductivity (pýmhoslcm) zj4 A'3--
Temperature (0 m
4 DiH (S.U.)
r J
i DO (ma/L) 86.4%
Conductivity
(ýmhos/cm) 10-9
.1 k-Temperature ("M S
S pH (S.U.)
DO (mg/L) rd, D Conductivity 100%
(Amhos/cm)
Alkalinity (mg CaC031L)
Hardness (mg CaC03/L)
Temperature ('C)
U-4 i
v I
oH (S.U.)
II',
--. 1
- ~-~-*-----
DO (mgfL)o mm 100%
Intake Conductivity I~
Alkalinity Hardness-TRchorine (g/L Temperature ('C) 24 1
13 I" q i, 1 7tt 7
"tq.
q
- 2. 19 I
ma Initial II Final Initial Final I
Initial 11 Final I
Initial
))
Final l
I Page 40 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
En Tom ItS ~
Iii -
Page 1 of 7 I
I I
i i
I i
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0026450 Project#:
1011A County: Hamilton Outfall: 101 Dilution preparation information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information-Test information:
Organism age:
< 24-hours old Randomizing template color:
6.d%,]
Date and times organisms were born O$.G3P-%% 6"K T1 Mot Incubator number and shelf between:
locaion Culture board:
2**\\
location:
Replicate number:
I1 2
3 4
5 6
7 9
10 bac Culture board cup number: I Ll I S I
I\\ I l
A I
q 11 YWT batch:
o.l '-
Transfer vessel information:
pH= 8.00 S.U. Temperature= 3A.
°C Average transfer volume (mL):
S.*
Selenastrum batch:
o4.1 I[
I I
Daily renewal information:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake termination time I
0 bk4 os-a-11 A
'LOSca. 0 lkOo2.a2..
1 C6.L4-it tv12 OS.f5 -itQ k 10o2.iL.
O 1
11oSoT..
2
_____it_
12tO
_05GLjLja la0sog42 Ir4SO4..2_
3 05-06-4
\\%!as_
t,.(-k
%* O' oI.
t I %lS09. 2:L 5
c0oo.1%*
I 21(at I 5-.*-t1 I it o I., is t* oe.
0%. -1 I
I 6
c&S.cA1 i -.L 7
I __A*_1b-1( I t -L-Lý Control information:
Summary of test endpoints:
_ Control-I Control-2 Acceptance criteria
% of Male Adults:
07-
- 07.
!5 20%
7-day LC50 ICol.
% Adults having 3ra Broods:
- tool, 100?.
- _80%
NOEC l0o*.
% Mortality:
- 07.
20%
LOEC CO_______
Mean Offspring/Female:
al
-. 15.0 offspring/female ChV I t
%CV:
4.1.1.
1_ _.8_
<40.0/%
IC25
'7100".*
I Page 41 of 100 SOP ATI I - Exhibit AT 11.2, revision 04-01-09
a
-~ t,.i.o naaa..O aSora n n Page 2 of 7 I
I I
I Species: Ceriodaphnia dubia Client: TVA / Sequovah Nuclear Plant, Outfall 101 Date:
CONTROL-I Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Young produced 0
C0.J~
~ ~
0 Adult mortality.
t r
LI I
L-L.
L.
3 Youngproduced [.J 0
0 C)I 00
.Adult mortality L
1I -
V.-I 4
Young produced L_
Adult mortalityI 1-_l*
L 5
Young produced I
[ t I
i".
Adultmortality J
.- K -
I L ILII--
6 Young produced
")
CI_0 C)
I I
0
[
Adult mortality oI
[
L Ik-L V_
7 Young produced v4 1-4 11. l
%S I
v, tA t*
v, s
Total young produced U_
,.1 3
3 Ta k
- z.
k
,.4 Final Adult Mortality 3
t I
\\
X for 3rd Broods
-A 54-X*
Mote: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO - carry over (offspring carried over with adult durng transfer).
IConcentration:
% Mortality:
I Mean Offspring/Female:
I 7.m l
CONC: 10.8%
Survival and Reproduction Data Replicate number Day i T 2
3 4
5 6
7 1
9 1
10 Young produced
- 0.
0 Adult mortality_
t 1 1 k1 2
Young produced[I i[
0]
0I 0[
Adult mortality j
\\.- I-I-
[*
lJ ll-3 [Young produced
~
~
~
t
~
~
0 4 [
ngrdAdult mortality f.,___
I _
4 Young produced S
A S
'-A I -Adult mortality I
I-L I
I I LtL
]
5 Young produced I IS lo I
-- t I
Adl
-otlt I-I L
- I
-t--
tI--
Total young produced mo
.30 iA 6
.y 1-sa at ot wits au
.s o n2r.
Final Adult Mortality
,k..
L..
Notbe., Adult mortality (L -uvce D -
ead), SB= splittbroodo(single brood splitbetween two daysi, Cv -ca over kous fnneamreoovery tna- --
" n--ras-er-LConcentration:
% Mortality:
07 I
Mean Offspring/Female:
1
'30A
% Reduction from Control-I:
1-q' 07l SOP ATI I - Exhibit ATI 1.2, revision 04-01-09 Page 42 of 100
II tniirowi.otaimoiogs.osoni.iet Page 3 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 CONC: 21.6%
Surv Date:
CA-CM. 1A, ival and Reproduction Data Replicate number Day 1
2 3
4 5
61 7
8 9
10 I
Young produced r
j 0
c C
C.
_ _ Adult mortality I.
V I
__ I 2
Young produced
(
)
OF I
Adult mortality L"-']
i-I *l
-I V -
3
~Young produced o
0 Adult mortality 1
I U'.l 4 Young produced 12A
%4 q.XIL I'
'4.
%4
- 1. '4 Adult mortality
~
~
~
~ t~
-I~
5 Young produced Adult mortality 1"
I 6
[Young produced Qj 0
0 Adult mortality t
U U
k UJ_.
7 Young produced
[=
1%
-I Total young produced
- a.
\\
"L 3(
I 3
W.
T-31 I-I-Final Adult Mortality
\\-
I
\\.-
I
\\_
Note: Adult mortality (L - live, 0 = dead), SB = split brood (single brood split between two days), CO -ca over (offisprisn carned over with adult during transfer)
Concentration:
% Mortality:
07.
Mean Offspring/Female:
I
,°i
% Reduction from Control-I: 1 CONC: 43.2%
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 TI Yon prdue 5
5 Fc 1
onpoueAdult mortality
(.
ý)
I \\_
k L_*
L 2
Young produced Q
C Q
C[
Adult mortality I'
V__
I
- 1.
- 1. %,
W 3
Young produced C>
IC)o I_0]oJ 0
0 Adult mortality I 3
Youngproduced ta J[
j 0 i
]...*_
t Adult mortality
ý..
U_
1 L.i Adult mortality.
-l 4
Young produced t
0)4n 2
04 E
Adult mortality L_
01 1-L_
7 Young produ-ce-dTti I
ISTjk.-ct-r__ t4%
%I IS Total young produced as is cI q
2.-
- Final Adult Mortality I
i..
' _ I '_
I Note: Adult mortality (L = live, D - dead), SB - split brood (single brood split between two days), CO = carn over (offtspring carried over with adult during transfer).
I I
I Ii Concentration:
% Mortality:
0:7.
Mean Offspring/Female:
31h.
% Reduction from Control-1:
- ISJ.
I Page 43 of 100 SOP AT II - Exhibit ATI11.2, revision 04-01-09
n 5f Page 4 of 7 Species: Cerlodaphnia dubia Client: TVA / Seauoyah Nuclear Plant, Outfall 101 CONC: 86.4%
Surv Date:
OS-0"3-tk ival and Reproduction Data I
I I
Day 2
3 4
5 6
7 8
9 10 SAdult mortality t*
I 1
Young produced 0
0 0
,1O 00d Adult mortality t--
3 Young produced 0
C.
Adult mty o
Q-1-
4 Young produced~ s SI_~T.~
Adult mortality L.
U 5
Young produced 1l
%115 I
I A
3 O
IS Adultmortality L
-*j L-U-J--
L.IL 7 Young prdu 16
- k.
-Lo
-L lot otL 7
onproduced l*
l t
"L.-O*-
".'7-.1
\\
Total young produced S
56 C
1 56 as O s
Final Adult Mortality
\\._
I M
]
j Note: Adult mortality (L = live, D = dead), SB = split broad (single brood split between two days), CO = cart, over (oftsring carried over with adult during tramrser).
Concentration:
% Mortality:
0 7, Mean Offspring/Female:
34~A
% Reduction from Control-I:
- r'1.S7, CONC: 100%
Survival and Reproduction Data Replicate number Day 2
3 4
5 6
7 8
9 10 I
Young produced 0
]
(
)
C I 0 I° Adult mortality C
2 Young produced cQj C)
I r) 0 0
0 0
0.......
Adult mortality [L-..L
,-_-.1
.-1.. l I
- 1.*
3 Young produced [
n
)
=
Adult mortality I
4 Young produced Adult mortality [_
VI J _
LtI--_'.--I_
5 Young produced jA
-L
- J"
ý1 1 I
O Adult mortality
_ %--I--+~-~
L 2-I+/- \\
6 Young produced r)f I
o o__L..J__
IAdult mortality li 7
Young produced i
Lj
~
1,t Total young produced
- j.
as S.
3 (6 3 6
5*
Final Adult Mortality
- t.
c..
j.
I te:l¢ M*IUlt mlortltlly Ik. ý Ilive, D = dead), So - splitl brood (inglt*e broodI split between two da~ys), CO. = C4rI, over (offlOn ca**
ei over wits**adlt dnuorg trantser).
Concentration:
% Mortality:
07.
Mean Offspring/Female:
3I.4
% Reduction from Control-1:
,, 7, I
Page 44 of 100 SOP ATI 1 - Exhibit ATI 1.2, revision 04-01-09
T I
,.* E*V&luenmmnimtlTnlagsauo'utihint.l Page 5 of 7 Species: Ceriodaohnia dubia Client: TVA / Seq uoyah Nuclear Plant, Outfall 101 CONTROL-2 Surv Date:
oS-qf*-%X ival and Reproduction Data Replicate number Day 1
2 3
4 5
1 6
-7 8
9 10 Adult mortality__
k-
\\-
- I__
,-I--
1 Young produced 0
Adult mortality t-1 1i 1
Adult mortality U..
U"
'-.,U-U U
U-4 Yongduied I O.
4 U _
S 7 Adult mortali 7I 5
Young produced k
-1 I
t*
b k
Adult mortality 1
- 7.
]
1t 1
.'-.K I--
6 Young produced 1 5o C) 0~~
lI1~
7 Young produced k~.O.C S
u-T o ta l y o u n g p r o d u c e d 2 0!t 7. o W A
- o
".A 1 1.S 3 ".
- 3 k 7-Ij Final Adult Mortality
=
C--
C t.-
\\...
X for 3'd Broods
"..~...*
I-
- I Note: Adult mortality (L = live. D = dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
IConcentration:
% Mortalit:
07.
Mean Offspring/Female:
0 CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1
2 3
4[
5 6
7 8
9 10 I
Young produced C) 0 Adult mortality V-[
U U
U J
I 2
Young produced 101 0 DL L
J i
Adult mortality L
IU L
LU
' 0 I O 3
Young produced
___IAdult mortalityU
-U U
jU Young produced
%4 I
O
{
IO Adult mortality
\\-
U b-..---K-i-I'I U I
[
5 Young produced t lo I'L I4 cLVL
)lj 1,3 Adult mortality I
ýX %'L L.
L L
6 Young produced 6
Adult mortalityI L1 T1..__-I L-L..
7 Young produced T 1 X
k 10
'"(
I I&
tot 21 1 _
Total youngproduced s
s j
- -i I
2.. 1 2b,=
3 3L,,
Final Adult Mortality
- i.
I I
le: MtuUt mortaltt) k, = ive, D/ - deaul, SB - spllt brord]l kSlinle brood spill betweenl two dasy), COU =
-overl *.to p nlll c;tllt olverI u¢ ttU U =Itg twls-).
Concentration:
% Mortality:
O*
Mean Offspring/Female:
35.
% Reduction from Control-2:
-- o.s7.
Page 45 of 100 SOP ATI 1 - Exhibit AT 11.2, revision 04-01-09
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011 ETS E vo n e W e t fgoh f h ~ I Verification of Ceriodaphnia Reproduction Totals Control-i Day Replicate number Total I
L 3
4 5
6 71 8 1 T9 10 Ta i
0 0
0 0
0 0
0 L
0 0
-0 0
2 0
0 0
0 0
0 0
J0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 3
4 4
5 4
4 4
4 4
40 5
10 11 11 12 11 12 12 10 12 12 113 6
0 0
0 0
0 0
0 0
0 0
0 7
14 14 16 15 15 15 14 15 13 13 144 Total 28 28 31 31 31 31 30 29 29 29 297 10.8%
DyReplicate number Total I
0 0
0 6
7 08 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0
.0 0
0 0
4 3
5 5
4 4
5 4
4 4
6 44 5
13 10 13 12 10 12 12 10 11 11 114 6
0 0
0 0
0 0
0 0
0 0
0 3
7 1
6 1
3 16 1 4 1 4 1 5 15 '5 1 25 1 Total 33 1,30 34 32 27 33 30 28 30 32 309 21.6%
Replicate number Total Day 1
2 3
4 5
6 7
1 8 1 9 10to 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0-0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
3 4
4 4
4 4
40 5
12 lO 12 13 12 12 12 12 11 11 117 6
0 0
0 0
0 0
0 0
0 0
0 7
17 17 15 19 14 16 15 5
16 18 162 Total 33 32 31 36 29 32 31 31 31 33 319 43.2%
Dy 1 2
Replicate number 8
0 Total Day 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
5 4
4 4
3 4
4 4
42 5
11 12 14 11 12 12 13 13 13 12
.123 6
0 0
0 0
0 0
0 0
0 0
0 7
19 16 15 15 19 20 19 17 15 18 173 Total 36 32 34 30 35 36 35 34 32 34 338 86.4%
Day p -
Renlie*q te number Da -y-r number Total 3 14 1 5 I 6 7 1 8 1 9 1 10 0
0 2
10 10 0 1 0 1 0 10 0
0 0
0 0 10 10 10 10 0
0 0
3 0
0 0 10 10 10 0 !0 0 10 0
4 5
4 5
5 4
4 6
4 6
5 48 5
13 12 12 14 12 11 I1 12 12 12 121 7
16 19 16 16 20 22 19 19 15 18 180 To,'I 34 35 33 135 136 137 136 135 133 15.4 49A 100%
Day
]~
U8
'A
!t!N*I Total I
2 13 4
6 7
8 9
1 0 a1y 0
o D1 0 2
0 0 10 10 00 1 0 0 0 1 0 3
0 0
0 10 0
0 0
0 0
0 0
4 5
5 6
5 5
4 6
6 4
5 51 5
14 12 1313 12 14 12 12 12 12 126 6
0 0
0 0
0 0
0 0
0 0
0 7
19 19 17 19 18 20 18 18 20 19 187 Total 38 36 36 37 35 38 36 36 36 36 34 Control-2 Day
~Replicate numberToa 1
2 3
4 0
5 6
0 7 8
9 10 T
1 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
3 5
3 2
30 32 3 4
4 36 2
12 10 12 11 I0It 11 12 12 10 112 6
0 0
0 0
0 0
0 0
0 0
0 7
3 13 17 16 15 13 15 17 115 15 1 149 Total 129 26 134 130 29 27 30 32 31 29 1 297 1
100% Intake Day
~
Replicate numberToa I_[ 2 4 3
4 5 16 7
8 49 14 40 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
L0 0
0 0
0 4
6 4
5 4
5 5
4 4
4 4
45 5
13 12 14 It 12 12 10 13 13 If 121 r?
0 0
0 0
0 0
0_1 0
1 7
18 21 16 20 20 18 18 19 21 21 192 Total 37 37 35 35 37 35 32 36 38 36 358 Page 46 of 100 File: sqnl0l_050311data.xlsx Entered by: J. Surmner Reviewed by:
d
0
- 3 Environmental Testing Solutions. Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
7068 Concentration Replicate number Survival Average reproduction Coemcient of Percent reduction from
(%)
1 2
3 4
5 6
7 8
9 10 1%)
(offspring/female) viation (%)
control (%)
Control - 1 28 28 31 31 31 31 30 29 29 29 100 29.7 4.2 Not applicable 10.8%
33 30 34 32 27 33 30 28 30 32 100 30.9 7.4
-4.0 21.6%
33 32 31 36 29 32 31 31 31 33 100 31.9 5.8
-7.4 43.2%
36 32 34 30 35 36 35 34 32 34 100 33.8 5.7
-13.8 86A%
34 35 33 35 36 37 36 35 33 35 100 34.9 3.7
-17.5 100%
38 36 36 37 35 38 36 36 36 36 100 36.4 2.7
-22.6 Control - 2 29 26 34 30 29 27 30 32 31 29 100 29.7 7.8 Not applicable 100% Intake 37 37 35 35 37 35 32 36 38 36 100 35.8 4.7
-20.5 Outfall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
MSD =
PMSD=
1.698 5.7 1.569 5.3 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90t percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-M1-005, US Environmental Protection Agency, Cincinnati, OH.
Page 47 of 100 File: sqn101_050311 data.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Sumrer Reviewed by:
ILA
I
. 4
.. i 0
EvTSo iý Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 03-10, 2011 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/3/2011 Test ID:
CdFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Cone-%
1 2
3 4
5 6
7 8
9 10 Control-1 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 Intake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed I-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean Control-1 29.700 1.0000 29.700 28.000 31.000 4.214 10 32.933 1.0000 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10 10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10
-1.616 2.287 1.698 32.933 1.0000 21.6 31.900 1.0741 31.900 29.000 36.000 5.809 10
-2.963 2.287 1.698 32.933 1.0000 43.2 33.800 1.1380 33.800 30.000 36.000 5.717 10
-5.523 2.287 1.698 32.933 1.0000 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10
-7.005 2.287 1L698 32.933 1.0000 100 36.400 1.2256 36.400 35.000 38.000 2.654 10
-9.025 2.287 1.698 32.933 L.0000 Intake 35.800 1.2054 35.800 32.000 38.000 4.711 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.829582155 1.035
-0.138685175 0.300894097 Bartlett's Test indicates equal variances (p = 0.13) 8.592660904 15.08631706 The control means are not significantly different (p = 1.00) 0 2.100923666 Hypothesis Test (I-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 I
1.697549464 0.057156548 64.58666667 2.755555556 1,9E-12 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples)
Point SD 95% CL Skew IC05
>100 ICI0
>100 IC15
>100 IC20
>100 lIC25
>1001 IC40
>100 IC50
>100 Page 48 of 100 File: sqn ! 01 05031 ldataxlsx Entered by: J. Sumner Reviewed by:
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 03-10, 2011 "EnI 0
Environmental Testing Solution s Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/3/2011 Test ID:
CdFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Diseharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-%
1 2
3 4
5 6
7 8
9 10 Control-I 28.000 28.000 31.000 31.000 31.000 31.000 30.000 29.000 29.000 29.000 Control-2 29.000 26.000 34.000 30.000 29.000 27.000 30.000 32.000 31.000 29.000 10.8 33.000 30.000 34.000 32.000 27.000 33.000 30.000 28.000 30.000 32.000 21.6 33.000 32.000 31.000 36.000 29.000 32.000 31.000 31.000 31.000 33.000 43.2 36.000 32.000 34.000 30.000 35.000 36.000 35.000 34.000 32.000 34.000 86.4 34.000 35.000 33.000 35.000 36.000 37.000 36.000 35.000 33.000 35.000 100 38.000 36.000 36.000 37.000 35.000 38.000 36.000 36.000 36.000 36.000 Intake 37.000 37.000 35.000 35.000 37.000 35.000 32.000 36.000 38.000 36.000 Transform: Untransformed I-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Control-I 29.700 1.0000 29.700 28.000 31.000 4.214 10 Control-2 29.700 1.0000 29.700 26.000 34.000 7.784 10 10.8 30.900 1.0404 30.900 27.000 34.000 7.388 10 21.6 31.900 1.0741 31.900 29.000 36.000 5.809 10 43.2 33.800 1.1380 33.800 30.000 36.000 5.717 10 86.4 34.900 1.1751 34.900 33.000 37.000 3.687 10 100 36.400 1.2256 36.400 35.000 38.000 2.654 10 Intake 35.800 1.2054 35.800 32.000 38-000 4.711 10
-6,741 1.734 1.569 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.949571669 0.868
-0.13778615 0.558140392 F-Test indicates equal variances (p = 0.36) 1.87890625 6.54108572 The control means are not significantly different (p = 1.00) 0 2.100923666 Hypothesis Test (I-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.569196635 0.052834904 186.05 4.094444444 2.6E-06 1, 18 Treatments vs Control-2 Page 49 of 100 File: sqnl0l_05031 ldata.xlsx Entered by: J. Sumner Reviewed by:
I.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 03-10, 2011 o
OEnvironmental Testing Solutions, Inc.
Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses Project number:
7068 m
Concentration lParameter 6
I DH (SUl Control DO ImpJLI zz91 Conductivity (umhos/cm)
I Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
A.
^1 m
______Temperature CC) 2449 4 247 24.9 24
.291 24.91 24.91 25.0 249.
29 24.81 25.2 24.91 25.21 PH (SU 7.855 77 7.80 790
.881 7.851 7.86[
7.86 7.82 771.93 71 7
801 7800 DO (umgL) 7.88 7.6 775 7.51 7.71 8.2 78.778 7.91 7.8 7.87 18% Conductivity (Lwnhos/cmn) 310) 311 283 288
- 29) 307 303 oH24,9 784 7717 9
8 8
8 8
8 9
9 8
8 7
________Ternperature
('C) 1 25.0 2
25.0 24.9 29 25.0 250.
4820 2520 24.9 2515.
25.0 pmH(U) 777 7764 7.779
.9 7.86 7.86 7,845 8
7.84 70 7.9238 7.8127 2.% DO (mg/L) 7.7 7.6 7.7 7.6 7.8 7.8 801 7.8 7.8 8.0 8.1 7.8 7.8 7.7 42% Conucntivit pmnlos/eni 252 257 272 241z
-245 254 2853
______Temperature
(*C) 25.0 2519 25.0 24.9 25.0 2510 25.01 24.81 25.0 2502 24.9 25.1 25.0 25.1 pH (U) 7.72 7.74 7.73 7.86 7.801 7.86 7824 7.861 7.82 7.93 7.921 7.831 7.79 7.78 86.4%
DO nnaL) 787 7.6 7.7 7.6 7.8 7.8 8.0 778 7.8 8.0 801 778 7.8 7.9 Conductivity (1umhos/cm) 174
ý 183 218 18748 207 189 Temperature ("C) 25.0 24.8 25.0 24.9 25.0 24.9 25.1 24.9 25.0 25.0 24.9 24.9 25.10 225.2.
PH (SU) 7.72 7.72 7.72 7.85 7.76 7.86 7.81 7.86 7.81 7.93 7.90 7.83 7.79 7.77 8.% DO (mpIL) 7.8 7.5 7.8 7.61
- 78.
7.8 8.0 7.7 7.8 8.0 8.0 7.8 7.7 8.0 Conductivity bimhos/cm) 1579 189 158 1587 166 1 274 171 Temperature ("C) 25.0 24.81 25.0 24.8 25.01 25.21 25.2 24.9 25.0 24.9 24.9 24.9 25.1 25.2 9H(U) 7.69, 7.721 7.72 7.83 7.751 7.841 7.811 7.85 7.82.
7.91 7.92 7.83 7.789.7 DO (mgL) 7.81 7.61 7.8 7.7 8.01 7.81 8.01 7.8 7.91 8.1 8.11 7.7 7.87.
Conductivity (jnmhoslcm) 155 15 15715 17017 172 100% Itk Alkalinity (mig/L CaCO3) 55616 Hardness (mg/L CaCO3) 5 36 Total Residual Chlorine (mg/L)
<0.1 00
<0.0
_______Temperature
(*C) 25.01 24.91 24.91 24.81 24.91 25.21 24.91 24.8 24.91 24.91 24.91 24.91 25.1 25.2 Page 50 of 100 File: sqn101_05031 lchem.xls Entered by: S. Evans Reviewed by:
EM,.m
~.Th~gS
~
Page 6 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Daily Chemistry:
Date:
OS-f6-'
Day 12 Concentratio n Parame CONTROL Alkalinity (mg CaCO3/L)
Hardness (me CaCo 3/1,)
__________Temperature
('C) i l
oH (S.U.)
310 14411 r~.'A9')
l~Oh~
I
.1~~~l V
LL!IL.L9 J
DO (ma/L) 10.8%
Conductivity I (jimhos/cm)
Temperature (C) 9 9-pH (S.U.)
U.l-0 UI 0I.S,,
I A~
1 DO (mg/L)
I 21.6%
Conductivity (iimhos/cm)T Temperature (C)
I." t d
I D.
oH (S.U.)
'~JIL1L~L' I "J~-~
!I2rAO DO (mr/L)
Ii
--I 43.2%
Conductivity (Prnhos/cm)
IEI1 Temperature (°C)
VSI 1Ls.I I
i ~
oH (S.U.)
II 44
- -+.
++,
- 1-73Z DO (mp/L)
ME 86.4%
Conductivity (jimhos/cm),
Temperature (°C) u Ii ii 9
'I ~
pH (SU.)
"-4',-1 II *1.
l.===..,.--
r DO (mp./L)
"-0 II 100%
Conductivity
..pmhos/cmn) 7 -"
Alkalinity (mng Ca(-O3/L) 5A Hardness (mg CaCh3lo (g/L)
<Q, 11D 40.10
.S l
".6*.1I I l* '
Temperature (°C)
"L.o0 II.L4.f I
p oH (S.U.)
"J.df II 1~5I 100%
Intake DO (mg/L)
Conductivity (Mumhos/cm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCOt3/L)
(0.
q.j
(0,10
- i.
="
Temoerature (°C) 7S 0 II I
Initial Initial Final Initial Final Page 51 of 100 SOP AT 11 - Exhibit AT 11.2, revision 04-01-09
E ~to.,., 1~ U ;S ~
Page 7 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date:
O'S-*-b-\\
I Dav I
Day I.
v-4 I
5 Analyst Concen-Parameter trtio PH (S.U.)
DO (mg/I)
Conductivity (gmhos/crn)
CONTROL Alkalinity (mg CaCO3IL)
Hardness (Mg CaCO3L)
Temperature (MC)
~.
pH (S.U.)
DO (mg/L) 10.8%
Conductivity (grmhos/cm) 10 IVA
-4_.
- 97.
e V 8 1 II ~ ~ ~ L "0*,
I- "-s*
t
.'.L II".*
I"otII Temperature (°C)
U W
~.
.1 PH (S.U.)
- 7r.Q9 1I,g k1 11 Wq! I 120 11 7.ARlI 1 7,Al11Z 1*
I U-DO (m./L) 21.6%
Conductivity J
(4fmffiS/CM)
.1 LMLThJ 2.9-S 179 IRS+
155 L
-ts. C)
"I Pj I Tempue (c) i -
pH (S.u,)
iL9~4.1 ~.84 II DO (me/L) 43.2%
Conductivity i
_(grhos/cm)
Temperature MC) a pH (S.U.)
-Le I -I1 7i.153 DO (m/L) 86.4%
Conductivity (jimhos/cm)
Temperature ('Q PH (S.U.)
1 II 4
I I
I I'M 1
I 9';i II I.
I I-DO (mn-/L)
~.-
100%
Conductivity (Amhos/cm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Temperature (MC)
A 0.1b
-AA --
L-1 S
S -
pH (S.U.)
I "
it
.l'6 1 -. 4 I 4,t-a16 1
DO (mE/L) 100%
Intake Conductivity Alkalinity Hardness TR chlorine (mg/i.)
- C.'1 I
Teemperature (M)
S U11 111
.7-4. -V 1 1 VC-11
'4 1 1S. 1 11 1b, I-I Initial-i Final I
7nitial I
Final I
Initial
ýFinal I
initial jr Final Page 52 oflO00 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09
0 Enviromental Testi ons, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Page 1 of6 Client: Tennessee Valley Authority Facility: Sea uoyah Nuclear Plant NPDES #:
eOý
ý TM O tc AS Project #: ___
County:.
tA ILoTo4 Outfall: 101 Dilution preparation information:
Comments:
Dilution prep (%)
10.8 21.6 43.2 86.4 100 Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2230 1960 1420 340 0
Interferences.
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
24 -IS 00...* OUS.
Randomizing template:
Date and times organisms OS1.-t4
- t. Q Incubator number and were born between:
shelf location:
Organism source:
G<-Cn.oI Artemia CHM number:
,4. S 'IA Drying information for weight determination:
Transfer bowl information:
pH =
-1. 16 S.U.
Date / Time in oven:
0S.O-%0..,
110 Temperature, "1.'L "C
Initial oven temperature:
Average transfer volume:
Date / Time out of oven: os-P-AI i41 0 - In.,4 Final oven temperature:
Total drying time:
IA-.KOlls Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 g tJ.\\l-loOu 1 S z.ol
,,Ho<aza m. os*-n-11 l oS'ut*- IL-o4co
.k 1*>
tSA 4-1 tt.0a,o 0
%ose.. n-as ý-,it A 2
I 0O.*
IS00 t3op If iIoScA.21 I IoS'.4...
oS.j'.-tlB6 5 oa.-d CAOo I3IOfO.
is
- t15o0, it.
e&0-og it 6
7V OS-'%JO.
4 it0SoL.. LS tiOSoL. ((
6e.a-l%
I I
- 16g2_1 4 Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
67--
20%
7-day LC50 t1 7.
Average weight per initial larvae:
0._ _1&
w, NOEC Average weight per surviving larvae:
0.L1
> 0.25mg/larvae LOEC
>1?7.
ChV
>t1617.
IC25
>tool.
Page 53 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
F TS SEnvironmental Testing Solutions, Inc.
Page 2 of 6 1
I I
I I
Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date:
neJa.L\\,i Survival and Growth Data Day CONTROL 10.8%
21.6%
A B
C D
E F
G H
I J
K L
0 to 10 10 10 1 to.[0 10 10
/0 to to I
0 to to to to to I0 to
/0 0 /0 I0 to 2
to0 I 1 0 to -
1to to
/01 0 toI0 to to to 3
to to 10 lo b
1(
C'1It 01t tO I( Q 0
to t
4 It) 0
/(
I0 ()
10 to to
/0 I0 to
/0
!(0 5
11D 10 10 1b U 0
1 U t)
/0 10 0 to t0O1 6to to. to
/0 16 10 10 lo
/ to
/0 10
/0 11,/
0
!0
/D 10 0 to to
/0 I A =Pon weight (mg)
Tray-color-cde::
Analyst:,,'r, _
- J0 I3.14(
%?,.4:-R I*.
.OA it.3, ts*.4~ 14,9*
14,64 14-.06 ts-
.t Date:
0 C01104 0
/
8 = Pan + Larvae weight (mg)
Analyst:
~
t I
Date:
OSg. 14. it {
m
~tz-(
a4 ' -
ý
.34
(-qtWKU C = Larvae weight (mg)
= B - A HandTcalulated.y clor
.0c ode::
Analyst:
1 3 DIe I
I I
I I
I I
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
L V 0.
"P Average weight per Percent reduction initial number of from control (%)
j larvae (rag) a a
b a
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 54 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I I
- FTS S nvironmental Testingsolutions Inc.
Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101, UV-treated Date: c&.%k Survival and Growth Data Day 43.2%
86.4%
100%
M N
0 P
Q R
S T
U V
W X
0 lb 1I C
Q 1 0I
) 10 0
10 iQ 'O
/0 tO 1
/0toD0 16 0
10 0
(0 0
/0 /0
/0 2
(o 1010 10(0 o
t (t 0o to /o to 10 to 3
ID 10 10 1(
10 (0
/0 t
o
/0
/0 /0 o,
4
,4 10 t o (0 1()
(0 (0
to 10 10 0
/o to 5
( C t L1 0
IIo 10 10
'1 ct 6
(01 0(0 t
)0 0 /O at O
'/0 7
() (()
A
() 1 `1, 1
O U
IL)
/6 A = Pan weight (mg) e Tray color code::
Mmw-r c Analyst:
- MItA, i0 4A4L 11A1,,
14.? I 15,-n I4,U, (4-.L4 ttS it.m-1I, A-.S Date:
0o. o4,.f B = Pan + Larvae weight (rag)
Analyst: _
_0t (i
0.41 1..
Date:
os.1*.!t MS5. Mt,.01*'
LL.L 2..L ; t. 3 L..o$ t-.(41 MZo q
MA
.5 t,.L' C = Larvae weight (mug) = B - A Hand calculated.
2.S t,
8'41 10.1tj i.-I.
,1-
"o*'
f.l.
I.
1 l1
.QL
(..(.b Analyst:
Weight per initial number of larvae (rag)
= C / Initial number of larvae Hand calculated.A.
0(*
AN~
Ana-yst:
I Average weight per Percent reduction initial number of from control (%)
OAS&
- o.&l 0:.1"a q.0.
Oa&0-larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 55 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
Envrnmena Teta luttons. Inc.
Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, U-V-treated Date:
n Survival and Growth Data Day 0
I 2
3 4
5 6
7 100% Intake Y Z AA B
D 10
/0 t lo10 ogl A
-,.611
- a a
A = Pan weight (mg)
Tray color code::
C Analyst:
Vk)4 Date:
o ct -o_-i B = Pan + Larvae weight (mg)
Analyst:
Date:
OG-14. n C = Larvae weight (mg) = B - A Hand calculated.
Analyst:.__.
a*.....
Weight per initial number of I
= C / Initial number of larvae Hand calculated.
Analyst:
i l
Average weight per [ Percent reduction initial number of from control (%)
larvae (m2)
I i
Comments:
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
Page 56 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
"ETS Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 U-V-treated May 03-10,2011 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
7068 Not for Compliance Assessment, Internal L or, OC Cmtmfle (%)
Replicate lnbtoIm obfed Final..
ber duov. A-Pweigitd(ewg B = Pa +Lae La-wewe t (mg) W41ttlSo-lring Monwa-ightI Caffiontjrbftio WeAklgn/
.ns1b.
Mean crr' Mm.rdettlt/.I C
r Poserelnof N
cotur. f-rn houe.
weoight(m
- A-B 8-beheoderre.(ea Sennidegftmberd (Mov."ss dtune(.j-(%1) mWereheeen necrntin. (.t.
etdA (%)
Ieave. (acw l
)
,6,,,
A 10 10 14.07 21,21 7.14 0.714 0.714 B
10 10 13.19 22.27 9.08 0.908 0*908 Con trl C
10 10 12.59 21.12 8.53 0.853 0.831 9.9 0.853 100,0 0.831 9.9 Notapplieable
_D 10 10 13.38 21.87 8.49 0.849 0.849 E
10 10 15.09 24.10 9.01 0.901 0.901 1
t0 10 13.35 21.48 8.13 0.813 0.813 10.8%
G 10 10 13.24 21.91 8.67 0.867 0.867 4.4 0.813 H
10 10 14.87 23.72 8.85 0.885 0.8s5 1
10 10 14.68 23.64 8.96 0.896 0.896 21.6%
J 10 10 14.06 21.99 7.93 0.793 0824 6.3 0.793 1000 0.824 63 0.8 K
10 10 13.16 20.95 7.79 0.779 0.779 L
10 10 13.14 21.43 8.29 0.829 0.829 M
10 10 14.76 23.55 8.79 0.879 0.879 N
10 10 14.42 22.94 8.52 0.852 0.852 0
10 10 13.76 22.23 8.47 0.847 0.847 P
10 9
14.39 22.12 7.73 0.859 0.773 Q
I0 10 13.83 22.33 8.50 0.850 0.850 86.4%
R 1 0 9
15.23 22.93 7.70 0.856 0.819 4.9 0370 97.5 0.798 4.6 4.0 S
to 10 14.26 22.00 7.74 0.774 0.774 T
10 10 14.64 22.61 7.97 0797 0.797 U
10 10 12.55 20.71 8.16 0.816 0.816
___0_
10 11.96 (9.98 8.02 0.8020.2 100%
lO 14.80 1.40 6.0 0.80 0.803 13.9 0.802 97.8 0.780 10.4 6.2 W
10 I0 14.80 21.40 6150 0660 0.660 X
10 9
14.85 23.25 8.40 0.933 0.840 Y
10 10 13.26 22.74 9.48 0.948 0.948 Z___
_____0 lO0 13184 22.67 8.83 0.883088 100% Intake z
t3014 22.83 9.25 0.850.911 3.4 0.883 97.5 0.889 7.4
-6.9 AA 10 LO 13! 5 22 9.25 0.925 1
0.9 0.925 OBB 10 9
14.80 22479 7.99 0.888 1
1 0.799 Out fall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
0.1009 12.1 0.1024 12.3 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difierence PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 126/6.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data firom EPA's WET Intemaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).
USEPA.
2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appcndix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnatin OH.
Page 57 of 100 Fie: sqrt10_050311data.-.xsox Entered by: J. Sumner Reviewed by -. A
I ; ý "ES
, Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 03-10, 2011 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/3/2011 Test ID:
PpFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-%
1 2
3 4
D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4
0.8488 1.0000 10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4
-0.848 2.410 0.1009 0.8488 1.0000 21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4
0.161 2.410 0.1009 0.8310 0.9791 43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4
-0.161 2.410 0.1009 0.8310 0.9791 86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4
0.795 2.410 0.1009 0.7978 0.9399 100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4
1.231 2.410 0.1009 0.7795 0.9184 Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4
Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.0 1) 0.93555665 0.884
-0.827227683 0.41674318 Bartlett's Test indicates equal variances (p = 0.63) 3.45981002 15.0863171 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
0.10085134 0.12136142 0.00376394 0.00350235 0.406950474 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point SD 95% CL(Exp)
Skew IC05 75.275 ICI0 ICI5
>100
>100 IC20
>100 IC25
>1001 IC40
>100 File: sqnlO_05031 ldata-uv.xlsx Entered by: J. Sumner Reviewed by:
IC50 Page 5810 f100
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated May 03-10, 2011 EiTS Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/3/2011 Test ID:
PpFRCR Sample ID:
TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Cone-%
1 2
3 4
D-Control 0.7140 0.9080 0.8530 0.8490 10.8 0.9010 0.8130 0.8670 0.8850 21.6 0.8960 0.7930 0.7790 0.8290 43.2 0.8790 0.8520 0.8470 0.7730 86.4 0.8500 0.7700 0.7740 0.7970 100 0.8160 0.8020 0.6600 0.8400 Intake 0.9480 0.8830 0.9250 0.7990 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 0.8310 1.0000 0.8310 0.7140 0.9080 9.930 4
10.8 0.8665 1.0427 0.8665 0.8130 0.9010 4.417 4
21.6 0.8243 0.9919 0.8243 0.7790 0.8960 6.341 4
43.2 0.8378 1.0081 0.8378 0.7730 0.8790 5.419 4
86.4 0.7978 0.9600 0.7978 0.7700 0.8500 4.614 4
100 0.7795 0.9380 0.7795 0.6600 0.8400 10.417 4
Intake 0.8888 1.0695 0.8888 0.7990 0.9480 7.382 4
-1.096 1.943 0.1024 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88838106 0.749
-0.9249599 -0.3380108 F-Test indicates equal variances (p = 0.72) 1.58184743 47.4683456 Hypothesis Test (I-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.10242295 0.12325264 0.00667012 0.00555646 0.31525472 1, 6 Treatments vs D-Control File: sqnll I
050311 data-uv.xlsx Entered by: J. Sumner Reviewed by:
Page 59 of 100
.........l........................
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated May 03-10, 2011 S
]Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Environmental Testing Solutions, Inc.
Project number:
7068 Concentration Parameter Day 0 Day I Day 2 1
ay 3 1
Bay 4 Day 5 Day 6 Initial IFinal Initial IFinal Initial IFinal IInitial IFinal IInitial IFinal Initial IFinal IInitial IFinal PH1 (SU) 7.781 7.78 7.78 7.73 7.891 7.761 7.861 7.741 7.781 7.8J4 7.911 7.661 7.831 7.69 DO (mg/L) 7.81 7.7 7.71 7.8 7.51 7.51 8.01 7.71 7.81 8..0 8.11 7.51 8.01 8.0 Conductivity (lumbos/cot) 324 2
9 0
0 0
1 Control Alkalinity (mg/I. CaCO3) 6 95 Hardness (mgIL CaCO3 )
96(09
_______Temperature
- C) 24.9 24.71 24.9 24.41 24.9 24.5 24.9 24.9 24.8 24.5 24.84 24.6 24.91 24.61 PH (SU) 7.79 7.781 7.79 7.73 7.89 7.76 7.88 7.76 7.84 7.85 7.92 7.66 7.83 7.70 DO (mg/I.)
7.7 7.7 7.8 7.3 7.7 7.5 7.8 7.8 7.8 8.0 8.1 7.5 7.8 7.8 10.8%
Conductivity (jimhos/cm) 306
~
310
-286
ý 291
ý 292 297 300
_______Temperature (1C) 25.0 24.7 25.0 24.5 24.9 24.8 25.0 24.6 24.9 24.3 24.9 24.6 24.9 24.6 pH (SU) 7.80 7.77 7.80 7.69 7.891 7.72 7.88 7.76.
7.86 7.86 7.93 7.66 7.83 7.69 2.% DO (mg/L) 7.7 7.7 7.8 73 7.8 7.5 7.8 7.81 7.8 7.9 8.1 7.5 7.7 7.9 2.% Conudctivity (jumbos/cm) 288 293 270 275 28 288 286
_______Temperature (OC) 25.1 24.6 25.0 24.5 24.9 24.6 25.0 24.6 24.9 24.2 24.9 24.7 24.9 24.8 pH(SU) 7.80 7.74 7.79 7.7 7.90 7.70 7.88 7.77 7.87 7.86 7.91 7.65 7.81 7.6 D O ( m g /L
)
7
.7 7.8 7.9 7 3 7
.7 7
.5 7
.9 7
.8 7 9 7 9 8
.1 7
.4 7.9 7.8 43.2%
Conductivity (pmhos/cm) 252 256 240 24.6 248 25522
_______Temperature C0C) 25.1 24.6 25.0 24.6 24.9 24.6 25.0 24.5 25.0 24.6 24.9 24.7 25.04 24.7 pH(SIJ) 7.79 7.71 7.75 770 7.83 7.691 7.88 7.74 7.84 7.87 7.90 7.62 7.81 7.66 DO (mg/I.)
7.8 7.8 7.9 74 7.9 7.51 7.9 7.7 7.9 8.0 8 1 7.4 7.81 7.8 86.4%
Conductivity (junhos/em) 178 186 179 18 192 197 195
_______Temperature
(*C) 25.1 24.6 25.0 24 24.9 24.61 25.0 24.7 25.0 24.5 24.9 24.5 25.01 24.7 pH (SU) 7.73 7.69 7.74 7.62 7.80 7.661 7.88 7.70 7.841 7.861 7.91 7.62 7.791 7.681 DO (mg/L) 8.0 7.7 7.9 7.4 8.0 7.61 7.9 7.8 7.91 7.91 8.1 7.5 7.91 7.91 Conductivity (jimhos/cmn) 154 158 5'5 7
17817 100%
Alkalinity (mg/I. CaCti 3) 52 615 Hardness (.ng/L CaCO3) 63 69.7 Total Residual Chlorine (mg/I.)
<0.10
<0.10101
_______Temperature CC) 25.21 24.71 25.11 24.61 24.91 24.51 25.21 24.71 25.2 24.51 25.01 24.71 25.11 24.71 pH (SU) 7.71J 7.681 7.73 7.66 7.781 7.69 7.78 7.751 7.851 7.851 7.901 7.631 7.781 76 DO (mg/L) 8.01 7.71 7.91 7.51 8.01 8.01 7.91 7.8 8.01 7.81 8.21 7.51 7.91 8.0 Conductivity (tusnhos/cm) 155 15I6 5
6 7
7 100% Intake Alkalinity (mg/I. CaCti 3) 52!96 Hardness (mg/I CaCO31) 63!97 Total Residual Chlorine L
I 01 01
<0.100
_______Temperature (C) 12ý 25.11 24.71 25.11 g24.6 j224.91 24.51 25.01 224.6 25.01 24.41 24.81 24.71 25.01 24.7 Page 60 of 100 File: sqn101_050311chem-UV.xls Entered by: S. Evans Reviewed by:
A
Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101. UV-treated Dailv Chemistrv:
Date:
OS-crn-i\\
Day Concentration Parameter Conductivity CONTROL mho/m)0!3 UV-treated (mg CaCO3/L)
I9 Hardness (mg CaCO3/L)
- "0 Temperature (*C)
-*'AA I
pH (S.U.)
,V49
. 9, I.-;ot
.13 I -Y 1:4,t I
DO (mg/L) 10.8%
Conductivity j
(Axmhos/cm)
Temperature ('CQ 21.6%
Conductivity (4mhos/cm)
_ Temperature (°C) 30(j
(
1.1. % 'LA-A
-VAAL.
_i I
II I
-r,
-I l
OtH (S.U.)
II ~
I 3.vi 1W R.,40 I
I+
()
II 1
1 DO (m/L) 43.2%
Conductivity (Amho s/cm) 4'. 'IS Temperature (°C)
'U F~.
pH (S.U.)
DO (mg/L) 86.4%
Conductivity 8
Temperature (°C)
U PH (S.U.)
DO (ma/L) 100%
Conductivity 1
(jrmhos/cm)
Alkalinity (mg CaCO 3/L)
Hardness 63 (mg CaCO3/L) (mgL)
TR chlorine (mgFL) 2-0, 10 Temperature (°C)
- ~.
-'-I I=,
pH (S.U.)
DO (mg/L) 100%
Intake Conductivity (A+/-mhos/cn) t 5
Alkalinity (mg CaC03/L) 52.
Hardness TRM CaCo 3 rin (_agL)
Ka'. (0 Temperature (°C)
ZS, i
Initial II Final I
Initial 1-Final I Initial 1t Final I
Page 61 of 100 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I
- vienrrMIl T So utlaos, Inc.
Page 6 of 6 Species: Pimephales promelas Client: TVA / Sea uovah Nuclear Plant* Outfali 101. UJV-treated Dnte~
r~.ni..qA Client TV
-/....
Nu la Plant...
ufl 10 U-trae Dae I
Day I
Analyst Concen-Parameter tration PH (S.U.)
DO (mg/L)
Conductivity (Lumhos/cm)
CONTROL UV-treated f9 Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L)
Temperature (0Q (3o1 8.0 "L*'°t I
aA'ItOIL It t
- -LA.*
"7q.B3 "L.
1, 1( 1 T.'/ W, 1 -oo 1 ý PH (S.
U.)
DO (mg/L)
Conductivity (umhos/cmn) 10.8%
I II I_
Temperature (mC) r pH (S.U.)
21.6%
DO (mg)
Conductivity
-(Amhos/cm)
P0 1
- 7.
%q.
Temperature ('C)
'U
'U---..
pH (S.U.)
111.
II ý,.-;--
1 R1,g", 11 ".-.-
I
- q
- Q 11 q
1 "7,81 I1' 43.2%
DBO (MRf)
Conductivity (iimhos/cm)
Temperature (0CQ
'U PH (S.U.)
9.9 I*
am t.
- ..'i
.9~
'.S'?
"4,q
"/.t
- 7. "5.1 IS-0 a
6,g uI
-I/ 01.
q II
- ,Lll 19"5 g
"L.q%.0 lq' l
a I
86.4%
DO (mg[L)
Conductivity
([inmhos-n)
Temperature (0C)
S pH (S.U.)
II bb
/o I
.R, 0
,4 1I 1S I -7 II.1%2 I ".71 Ii 3.10 I
100%
Conductivity
[\\i-q Alkalinity1 Hardness TR Chlorine (rag/L)
Temperature (°C)
Me -I*,L II 14:'
II "**'
S 16.c
.. "-0.
- l.
poH (S.U.)
Sq-,
II -4
- I-21e)
II 7-1,,-
I 7-7/B II IF 1-,,--',. -
DO (mg/L) 100%
Intake Conductivity (gtmhos/cm)
Alkalinity (Ing CaC0 3fL)
Hardness
-(mg CaCO3/L)
Temperature ('C) 7 I
C.O.(0
-I..
Fina Final I
Initial 11 Final In~itial Final I Initial nl
-z i
Page 62 of 100
.0 JtrO CO M
SOP AT20 - Exhibit AT20.3, revision 04-01-09
11 11 11 ii Page
?ae k OfJ.
Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter AnalystI IAi I
Date analyzed 10- 0. I1 I odide reagent: [ II q.* l_
Acid reagent:
[
1 qjt Calibration:
Nt:h r
a h
O
.10 mg/L
[
1.00 mg/L m
Slo re l
SRfrnestandard number I A1s91 J-I
,S59Z4 Note: For samples with a residual chlorine of > 1.0 rag/L, the catibi-ation range must be adjusted to bracket the chlorine levels of the samples.
I I
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
[ AS ssa o
0.50 Nf' Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = [(S - D) /[(S+D)t2l) x 100 number (m/gLL)
(acceptable range =E 10%)
Duplicate D
Sample measurements:_________________
Sample Sample I1D Sample characteristics Residual chlorine number m_____________
(gL)
Reagent Blank
--0,0(J03 43 1
0I 5 PCs
- i. (30 OW__,__IW.
a.-W.0_
p 5
' ive q.C I~* 3 u~*,po.e*o
&ti 0,4q5" UL612.ol VA'S- 0 101 rW W I W4-- LJ A-1,3(a Vote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
I W S) 9z1 0.50 0,'&
TO, 1/
PUD 11 Reviewed byIZ Date reviewed SOP C8 - Exhibit C8. 1, revision 09-01-09 Page 63 of 100
F Page Page Of 0V
)ET Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL ="0.10 mg/L Meter: Accumet Model AR25 pH/Ton Meter Analyst I W Iodide reagent:
Date analyzed 0G.06 11 Acid reagent: [I(NJ qP-4 i
Calibration:
0.10 mg/L I
1.00 mg/L I
Slope l
FReference standard number 7,A I tNSN'L.
1 -'solo Note: For samples with a residual chlorine of" 1.0 mg/IL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
0.50 o,~
Ou Duplicate sample precision:
Sample Sample 10D Sample characteristics Residual chlorine
%RPD ={((S - D) /[(S+D)/12) x 100 number I(mg/L)
(acceptable range -: 10%)
4 Duplicate D e t.O.L9 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number mL Reagent Blank
' DO WTI 1_
- 6. L
~~r.~
P-A7A1 OrV-C-3 5jk
",0ý
~1Q~SSIM.
11 ta Pik. iyqjlJ flOAY vJL~c L
4rtLu
<6, woz'.eI Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless oew ise noted.
L aboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS'=NMV /TV x 100 number I
(rag/L)
I(rag/L)
(acceptable range = 90 to 110%)j W-66
- 0.
Up.eO oo*
.0 2.0o(,0 Reviewed byI Date reviewed I
SOP C8 - Exhibit C8.1, revision 09-01-09 Page 64 of 100
Page 22.
Page of I
I I
I I
I I
II I
. Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumnet Model AR25 pH/Ion Meter Analyst [ fjljj Date analyzed [
i%*
2
.jtj Calibration:
Iodide reagent: lst.I*Z
+
Acid reagent: I-SwIA&I 0.10 mg/L 1
1.00 mg/L Slope I erencestandard number I"L s tk,*
5 C"I Z-46.9'1.
Aote: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
II Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
-0.50 c) 6Lj Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = [(S - D) /[(S+D)/21) x 100 number (mg/L)
(acceptable range = : 10%)
4,,
Duplicate D o
~o Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number I
(mgIL)
Reagent Blank
- 40. iy'i, ll..~tC 6
ri*.
0 an&Ag
-4r a
5
- a.
0 000MI 11______ka QTA~C.
0.LL 0 r&
5 G
[I Ii Ii Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
1..
Reviewed by I
ýq Date reviewed I OS-41-11 SOP C8 - Exhibit C8. 1, revision 09-01-09 Page 65 of 100
Envoyronmontal TestingSolutions, Inc.
page _.
___of Page Iof 4
Time initiated F D~o Z
Time completed L*I I
]
Analyst 1,Ii_.-
Date analyzed Lb.'I j
Titrant normality and multiolier determinatinu Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
,4 1'
I 1,
pH of Normality Normality (N) of HeSr 4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml Na2CO 3 x 0.05)/E
= (Nx 50000)/100 ml sample water reference standard ml ml ml
= 0.25/E
= N x 500
=4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Q-
"_142iA1 U
t-ý35C(Sr, D.
Q -
- 0. 0' 1 ql
__*.O1___
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End.
Total Multiplier (Mg CaCO 3/L)
(acceptable range (171 CaCO3/L)
(ml) ml ml ml
=90 to 110%)
TWSS 192-,
100 1oo Ia 1J
. q0 c
sq..B qol Duplicate sample precision:
loe ak" 9C."
[Sample
]Alkalinity I
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
(a -tb)./[range) 100 number
)
ml ml ml (acceptable range = + 10%)
SSW 9.+)2 30 Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) j CaCO/L)
(ml) ml ml ml
ýrW'S %93 too L"). -
05 Sample alkalinity (B)
Measured spike value (MV)
% R MV / SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (Mg CaCO3/L)
= 75 to 125%)
1 3'4 Sample measurements:
Sample volume Begin End Total Alkalinity Sample number gample ID (ml) ml ml ml Multiplier (mrg CaCO3/L)
Mc&t_*t I I rn4
-ý.* 1 1.3s S.q q.q 46ftzl4 oi1dA 1.3.5 0
.,, (9-0 1
-6Q, 1".5 k~ff C 6.b *. )
I W*
SS$IuA 1
Q l 2.l 3.2.
32..
-0.0 Reviewed by:
I]ý&
Date reviewed:
Page 66 of 100 SOP C6 - Exhibit C6. 1, revision 09-01-09
pI D
SEec '05mw. a Testing Solutions, Inc.
Page Lk Page Q off Time initiated Time completed II I
I Analyst I &7C Date analyzed I DE- 03. jI Tfrrant nnrmalilv and mulltinls'or datarnlnatiai Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
I I
I I
I PH of Normality Normality (N) Of H2S04 pH Factor or Multiplier Deionized Titrant cNEn--d--
E nd Total
= (5 ml Na2 CO3 x 0.05)/E
= (N x 50000)/ 100 ml sample water reference standard ml
-ml
"-i--ft-
.25/E
N x 5700ti' 4.5 S.U.
number number (E)
- (acceptable range 20)
Laboratory control standard.,.
Reference standard True value Sample Alkalinity (MV)
% RS = MV /TV X 100 number (TV) volume Begin End Total Multiplier (Mg CaCO3/L)
(acceptable range (a g CaCO3/L)
(ml) ml ml ml
=90 to 110%)
DIMS* tt6 100 100
)5-
?1:5:p.
C1.1.
q.9 go
.°}
Duplicate sam ple precision:
Sample Alkalinity
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)
[(S - D)/et(S+D)2l
) x 100 number (ml) ml ml ml I
I (acceptable range =:k 10%)
Duplicate (B)
-4 D
Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3/L)
(ml) ml ml ml 50z ID. (5 o) 3ý..q,
- 1..iC..
Sample alkalinity (B)
Measured spike value (MV)
% R MV / SV x 100 (Ing CaCO3/L)
MV = A - B (acceptable range (mg CaCO 3/L)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Itn CaCO3/L)
O~oiL
&t~S IA)S ilOs c:.*
fl.,,
jj.
Cj" 05M.z I t S,%
U24a 41-o 9A __
1 111 oS t
. 02-7-
- 33. -
+/-L D1
___4 I__III_
lUsal~t.C.
CI Utz 1
- 1. 1 l__
__O i
as,.c'--
11o1.1 2-0.L U
L CO 1 0 I-'I.ot
,i I,\\
,,,,e),?.7,
,(056,1o\\
3¢454, $tlv*
- . 1.'
&.5 I
Wl Reviewed by:
I KL-IZ Date reviewed: I 0*
q Page 67 of 100 SOP C6 - Exhibit C6.1, revision 09-01-09
S
)x.
Cnv ronmenta Testing So uions, Inc Page _
Page_.3 of '-
Time initiated Time completed Analyst t
Date analyzed L.*0.Ii Tifrani nnrmnlntv and mutnlan/
n/
dnats'rinajinn Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
_pI~-I
ý Nnrmallity'v T
Normality (N) of HSO4 pH Factor or Multiplier Deionized Titrant check Begin d'-
mNaCO3 x 0.05)/E
= (Nx 50000)/100 ml sample water reference standard ml all ml
=/
0 4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220) x 500 Laboratory control standard:.
Reference standard True value Sample Alkalinity (MV)
% RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L)
(acceptable range (mg CaCO3/L)
(ml) ml ml ml 90 to 110%)
..5w
- ?s 100
'00 I
q
-.2.
C11q
- 7.
g,,-..
Duplicate sam le precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
(S - D) /[(S+D)/21e 1 100 number ml) ml ml mll (acceptable range =
10%)
~hb~3A2A~ASga2.-
1 i
4~1.3Ž51 I Duplicate(B) 1 114.9 Itb
'R* 0 1 D
+
1 3.qo Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Mng CaCO 3/L)
(img CaCO3/L)
(ml) ml ml ml
,1 IV 5 D v
- e 141
-. Le 7*e.q
)50 Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO3'L)
MV = A - B (acceptable range (mg CaCO3/L)
= 75 to 125%)
Sample measurements:_______________
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml mi Multiplier (mng CaCO3IL)
I1, io.s
\\
4 3 N6D Z5 2.
-3.1f.(.01
- 26" 13I.'
a 3___
-5 (44.(,
LU oS, U,-1 *
- R O 0 1 1/60 R4.
,0.1'*
tU.i t
ns-40l*
.1
- 5.
.**-9o'**
o _.-
_. t-tel 7-1TA ZW~sQ AL \\&k3L 7--
.2' I%0So
.7.
I____
Ii (I
ii Reviewed by:
I Date reviewed: 10I5 i
-O Page 68 of 100 SOP C6 - Exhibit C6.1, revision 09-01-09
F a
SEniwronoetalsTesting Solutions, Inc.
Page 4-q Page __
ofJ4 Analyst p§;]
Date analyzed ID. CA -)I Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.5 S.U.
Time initiated Time completed Titrant normalitv and multiolier determination:
Normality Normality (N) of H2SO4 pH Factor or Multiplier Deicinized Titrant
-ch-e-ck-
-rgtn-
-- *L Tota (5 ml Na2CO3 X 0.05)/E
=(N x 50000)/ 100 ml sample water reference standard ml ml ml0.25/E N x 500
= 4.5 S.U.
number number (E)
(acceptable range_=_
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO3AL)
(ml) ml ml ml
= 90 to 110%)
100 100 9_.____._
__.___._______.O_
Duplicate sam ple precision:
Sample Alkalinity
%RPD Sample Sample iD volume Begin End Total Multiplier (mg CaCO3/L)
((S - D) /a(S+D)/21) x 100 number (ml) ml ml ml (acceptable range 10%)
Duplicate (B)
D I
3 Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3L)
(ml) ml mi ml J7 NSSV 3 100 5-J1.*
i4 q
) qi
ý.
150 Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (Mg CaCO/L)
MV= A - B (acceptable range (mg CaCO3/L)=
75 to 125%)
59 C1 I Cl. 0010 Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (6ig CaCO3/L)
%I
"(13Aa.Cn1* 1 f 31.4 3;4-2
(;b. q A 5e____
11 0S-A'so2'~L-Z
- 34.
.3-4-0
&D____9
_O,.l,,
3?1-,o AR,2. 3.Z,( L 1
- pPec, I
p~jal (nA
,,t
(.CI 1.0 121
ýo
__0 Id I__
IIwZO 0
Reviewed by:
I Date reviewed: b -16 Page 69 of 100 SOP C6 - Exhibit C6. 1, revision 09-01-09
EnvCiwronmvental Test ing Sltons, Ic Page Page _
_of -4 Total Hardness (SM 2340 C)
RL = 1.0 mg CaCOJL Analyst V L
- Date analyzed h
Ti trant nnrnali A and minl,Pnin, dAta*;i i*
Time initiated (Oq0Lj Time completed jii-
- L%)O~T af AI1%-eAWe LIMITS.
I I
I I
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E
= (Nx 50000)/50 ml sample number number (E)
(acceptable range = 0.0180 - 0.0220)
= Nx 1000
- 'r F,-423 JwSS; TIN f.t I qi let 20,,4oq Laboratory control standard.
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (Ing CaC03/L)
(ml) ml ml ml
=90 to 110%)
- S*9*q 40 50 Cj ;
ko-a;7 2.4-5/^"**
Duplicate sam e precision:
W.1
-2 t %A as
+81.S 7.
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (Ing CaCO5IL)
[(S - D) /[(S+D)/211 K 100 number (ml) ml ml ml___1 oS.os5,1 HlA 133*,q 2-..._*. s 35 I
Duplicate (B) 1-33 150 1 1.-4 1
D
_________/_
Matrix spike recoverv:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)
_(mg CaCO3/L)
(ml) ml ml ml lQ5 c 9o jJ1
-3.3 31-+
2-04 45 ý+(oJ Sample hardness (B)
Measured spike value (MV)
% R = MV / SV x 100 (mng CaCO3/L)
MV = A - B (acceptable range (mg CaCO3/L)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)
TV=ND Bank P.;
(should be = 0 mg CaCO3/L) cI-'.
0._
_0 05._-_
)
f Y)
U4.2.
t~LZI.II_____A---5
- 4. Z-
-6 ty4.&.3
.-1, 43.2, st 33.ZA,.__
13.-
3'.0o 4.9 Ss 06S -OZ-1 ki A_
33__
24--S 05.O.1 0.0
- 4.
1 3.j IS-_ 5 Li 1
O,*
3.T L0, II II Ii Ii II II II I'
_Note:'If_5m ~ttrantis used, sample must be diluted.
Reviewed by:
v g* 70 of-O Date reviewed I oa-O -I"I SOP C7 - Exhibit C7.1, revision 09-01-09
0 SE TS,--,
~ Environmen a
us a nt Sousas c Page :35 Page _*
of 4 Time initiated [
Time completed Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3 L Analyst 7 7 ]
Date analyzed [i;3i-i Titrant normality and multiplier determination:
Titrant NormaV ec-
-Bein& I El~d Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml l ml 2/E
= (Nx 50000)/50 ml sample number number (E)
(acceptable range =ý u'u0, "020)=
Nx 1000 Laboratory control standard:
Reference standard True value jSample Hardness (MV)
% RS =MV /TV x 100 [j number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO3/L)
(ml) ml mj ml
=90 to 10%)
Duplicate sa le precision:
ISample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (ng CaCO3/L)
{(S -D) /[(S+D)/21) x 100 number ml ml ml ml Duplicate (B) a
)
t.
4 I -+. 1.-
D t0 6,6 Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3/L)
(ml) ml ml ml 1'rss 1 Ck4t 5 D a-4 11o Lio
~4n 48 KIL(
Sample hardness (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO3/L)
MV = A-B (acceptable range (mg CaCO3/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV -
Blank (should be = 0 mg CaCO3/L)
I I
.3 0mo.
8D9 4
l1O$CP t.OZ-In -
/
-IT.
+
I_ :f-I____
10Zo Z
32..k 8(07 A4,1 1______
II 05s1. 0-3 k,
-d
.+
+/--.
I'0 lI I 2..1 Oi.
1 $
43%
?. -
5TS 1lO~c~(.0 l.
3 3*5 9
____5_
1I IOS.O2L--
'4A aw IýT
_3-5
'.o2.cl.
h I
FaOte: If.5m 1 t1t is used, sample must be diluted.
age/
O~i 0IU Reviewed by: I VI Date reviewed 1 QL".t SOP C7 - Exhibit C7.1, revision 09-01-09
4k I
Environmental Tellng Solutions, inc.
Page 3 (P Page
.3 of 4 Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst
- e.
i Date analyzed 05.o:-i Time initiated Time completed E I
]
Titrant normality and multiplier determination:
Titrant Narmaitv check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml I
= 0.2/E
=(N x 50000)/50 ml sample number number (E) le range = 0.0180- 0.0220)
= N x 1000 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mng CaCO3/L)
(acceptable range (Mag CaCO.L)
(m1) mI ml ml
= 90 to 110%)
-T5)S 9 1C1 40 50 L*..2
."-+
2o.,4 3'57 Q"4's."9.
1 Duplicate sam le precision: Ir el c,* *9 0
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L)
((S - D) /[(S+D)/21) z 100 number (ml) ml ml m
%I Dupi. cat(lB)
L 9 2.
12.1 O !
20.4 S Le I
Duplicate (B)
- 1.
11e,131 1
J-IDL I
I I
I I
I I
Samnie mesuemenft!
.... T m..
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCO 3/L)
T V *,
B la n k '
1Str.1 rIN*k so-to Im.5 6D..*,
i I **-5q 3o.3 4*4+
S, __-*\\I 30.3 at, -+ 4,L-II 1oAo II I-10100 1 13M 3.1._._
IIs'.s z
-49z1 ? 1 41l130Z.02-c
)
iL I3.i I~
~ ~ I
-Sq 72-31U-5I
- -y 1
0 I
-Note: If.,lSml,otit t is used, sample must be diluted.
P-age ( 0 UU Reviewed by: 1 Date reviewed 105-094, I
SOP C7 - Exhibit C7.1, revision 09-01-09
FETS5-SEnvIvoflmentaITesting Solutions, Inc.
Page 3_
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO31L Analyst[]
Date analyzed Q5-01" t Titrant normality and multiplier determination:
Page
- L4 offL Time initiated [
Time Completed I.- ZJ I
I ck--Nep-°fteclk Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml m
= 0.2/E
= (Nx 50000)/50 ml sample number number (E)
(acceptable ra-
.=0 N x 1000 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rag CaCO3/L)
(acceptable range (mg CaCO3/L)
(ml) ml ml ml
= 90 to 110%)
j55 q jt 40 50 1,.6 q.'-I 2-D q
-391 Duplicate sam le precision:
Sample Hardness
%RPD(=
Sample Sample ED volume Begin End Total Multiplier
((rg COco 3/L)
((S - D) /L(S+D)/211 x 100 number Ca_
L) (ml) ml ml ml
~ ~J
-~
'fl9.4.
12.1~
~
D Slicate (B)e%
D
-p CQ-I I
1 to 1280 Matrix spike recovens:
eference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3
/L)
(mg CaCO3/]L) 4... m14 ml ml
-Tjs 1i l40 D
%,~ 1a1L,. c>
-00 Sample hardness (B)
Measured spike value (MV)
%RMV/Sx10 (m=g CC 3 BL)
MV=nAkB (acceptable range (119 C*aC*/L)
=75 to 125%)
I
$D 10, Sample measurements:_______________
Smlnubr SmlID Sample volume Begin IEnd Total Hardness Sa I umer Sm c D(ml) ml nml ml Multiplier mgCaCO3JL)
TV WD.
Blank5
'(4_
llnii.~ ~ ~
~
cA qo(
C Urj-,L
+1
- z.
12
_90 3M
+ sc~
i
[=:oo~blfý
ýt~ft is used, sample must be diluted.
Reviewed by:
I Date reviewed 105s019-L
]
SOP C7 - Exhibit C7.1, revision 09-01-09
r CC-Envionmental Testing Solutions, Inc.
Analyst Date analyzed 0.&.
%te Rfft~b OW~ po~
-01 KAeIWbweSS LOGr.Pa SuA.t F~ee'-
~ltPage 1of tk f~_~'
eM.
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Time initiated I oq' I
Time completed I
Titrant normality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
- 0.21E
- (N x 50000)!50 ml sample number number (E)
(acceptable tiange = 0.0180 - 0.0220)
= NX 1000 I (Neqn (NS6e5 M86.0 Cie.0 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO3/L)
(acceptable range (rag CaCO3/L)
(m) ml mi ml ml
=90 to 110%)
I 3N lI 40 0
2I 100o'/,
Duplicate sam le precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (Ing CaCO3/L)
{(S -D) /[(S+D)/21) x 100 number (ml) ml ml m
Duplicat_(B)
W 11A 5q
.o 1,1 20.2 41.
o.E Duplicate (B)
I IjSj
.I I
I Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV = ND Blank" (should be = 0 mg CaCO3/
6
_ 6-0. 0.0 0 A 20.2-i 4-V Q3.L 3(a.0-A-oq~zl.*1.v
,,o '60.q 1
MS.. I
- __ *, 3 1 5.1 qL os*.
- di~i
~-
I p~l"f 5& of "pt is used, sample must be diluted.
Reviewed by:
Date reviewed I "0s,-
I SOP C7 - Exhibit C7.1, revision 09-01-09
Sequoyah Nuclear Plant Biomonitoring May 03 - 10, 2011 Appendix D Reference Toxicant Test and Control Chart Page 75 of 100
Pimephales promelas T
Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.
.a Environmental Testing Solutions, Inc.
1.0 0.9 0.8 0.7 USEPA Control Limits (+ 2 Standard Deviations) t I
l II I
I I
I I
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I I
I 0.6 0.5 1.4 1.2 U
1.0 Ln 0.8 N
0.6 0.4 1.4 I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I USEPA Warning and Control Limits (75th and 90th Percentile CVs)
I I
I I
II I
I I
I I
I I
I I
I I
I I
I I
I 1.2 1.0 Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 0.8 0.6 0.4 Test date
-. day ICzs = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
-Warning Limits (mean IC25 +/-
5 A10 or SA 7 5)
Graphs generated from associated excel spread:
..........Control Limits (mean ICs 25 +/- SA.S SA90' or 2 Standard Deviations)
Excel spreads*eet entered by: J, Sumner 5
A.
Reviewed by:
-I sheet.
j 5 -. %J1 I %J5.j
I.
I d
Ii.
i*.1M
~W 0
EnvironmeutalTesting Solutions, Inc.
Test number Test date 7-day IC.
CT (9/L KCl)
(g/L KCI)
Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Control Limits S&t Warning Limits SA25 CT-25 CT+2a CT-S&W CT + SA.s 0 Laboratory Control Limits S&
5a CT-sw CT+s.
USEPA Wamrig Limits S&90 aT-SA CTa+ S_,
USEPA Control Limits CT-SA..
CT+ SA.s Cv 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 09-14-10 0.75 09-21-10 0.73 09-28-10 0.65 09-29-10 0.68 10-05-10 0.70 10-06-10 0.72 10-12-10 0.70 10-26-10 0.68 11-02-10 0.70 11-09-10 0.75 12-07-10 0.77 01-18-11 0.78 01-25-11 0.70 02-08-11 0.69 02-15-11 0.68 03-08-11 0.71 03-08-11 0.70 03-29-11 0.75 03-31-11 0.75 05-03-11 0.78 0.74 0.71 0.70 0.70 0.70 0.70 0.70 0.70 0.71 0.71 0.72 0.72 0.71 0.71 0.71 0.71 0.71 0.71 0.72 0.02 0.71 0.77 0.09 0.65 0.83 0.16 0.58 0.89 0.28 0.05 0.60 0.82 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.05 0.61 0.79 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.04 0.62 0.78 0.08 0.62 0.79 0.15 0.55 0.85 0.27 0.04 0.63 0.78 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.03 0.64 0.77 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0.55 0.85 0.27 0.03 0.64 0.76 0.08 0.62 0.78 0.15 0-55 0.85 0.27 0.03 0.64 0.77 0.08 0.62 0.79 0.15 0.56 0.85 0.27 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.78 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.04 0.64 0.79 0.09 0.63 0.80 0.15 0.56 0.87 0.27 0.04 0.64 0.80 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.46 0.44 0.43 0.43 0.44 0.44 0.43 0.43 0.44 0.44 0.44 0.44
.0.44 0.44 0.44 0.44 0.44 0.44 0.45 1.02 0.33 0.41 1.07 0.02 0.98 0.32 0.39 1.03 0.08 0.97 0.32 0.39 1.02 0.07 0.97 0.32 0.39 1.02 0.06 0.97 0.32 0.39 1.02 0.05 0.97 0.32 0.39 1.02 0.05 0.97 0.32 0.39 1.02 0.05 0.97 0.32 0.39 1.02 0.04 0.97 0.32 0.39 1.02 0.05 0.98 0.32 0.39 1.03 0.05 0.99 0.32 0.39 1.04 0.06 0.99 0.32 0.39 1.04 0.05 0.99 0.32 0.39 1.04 0.05 0.98 0.32 0.39 1.03 0.05 0.98 0.32 0.39 1.03 0.05 0.98 0.32 0.39 1.03 0.05 0.98 0.32 0.39 1.03 0.05 0.99 0.32 0.39 1.04 0.05 0.99 0.32 0.40 1.04 0.05 Note:
7-d IC,, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the ICzs values.
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC2s values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
S&. = Standard deviation corresponding to the 10" percentile CV. (SAW0
= 0.12)
SAt = Standard deviation corresponding to the 25' percentile CV. (S,,, = 0.21)
USEPA Control and Warning Limits S,,, = Standard deviation corresponding to the 750, percentile CV. (Sa, = 0.38)
S.= Standard deviation corresponding to the 90" percentile CV. (So = 0.45)
CV = Coefficient of variation of the ICs values.
USEPA. 2000. Undersanding and Accounting for Method Variability in whole Effluent Toicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-e0-503.
US Emironmemtal Protection Agemicy, Cinnnati, OH.
Page 77 of 100 Organisms obanedrdfmc Aquaoox, Lim File: ppkcclk_0o3elt.xls Eatered by 1. Serum Hevi.esl by. _.
Environri 1.25 1.00 0 -2 0.75 W
0.50 0 bLE 0.25 I
nental Testing Solutions, Inc.
Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.
I-I I
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I USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
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I-Kentucky Acceptance Limit (< 30.0%)
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I ii ii i
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L v'o vo SO SO vo
-0 vo os-o ov" ýV*"';nvý Test date 0
Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD)
Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
Page 78 of 100 Graphs generated from associated excel spreadsheet.
Excel spreadsheet enlred by: J. Sumner Reviewed by:
S S
- ETS, Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Pimephales promelas Chronic Reference Toxicant Data Control Control Mean Test number Test date Sural C
rolth Survival Growth cr Cv CT MSD PMSD Cr for Control Growth
(%)
(g/larvae)
(m/arvae) for Control Growth CV (%)
(%)
for PMSD (%)
1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 09-14-10 09-21-10 09-28-10 09-29-10 10-05-10 10-06-10 10-12-10 10-26-10 11-02-10 11-09-10 12-07-10 01-18-11 01-25-11 02-08-11 02-15-11 03-08-11 03-08-11 03-29-11 03-31-11 05-03-11 97.5 100 97.5 100 100 100 100 100 100 100 100 97.5 100 100 100 100 100 100 100 100 0.813 0.891 0.991 0.893 0.912 0.810 0.799 0.779 0.727 0.761 1.080 0.806 0.608 0.817 0.701 0.953 1.006 0.829 0.675 0.867 0.852 0.898 0.897 0.900 0.885 0.873 0.861 0.846 0.837 0.860 0.855 0.836 0.835 0.826 0.834 0.844 0.843 0.834 0.836 5.9 7.5 9.5 8.5 8.1 2.3 4.1 10.0 10.9 6.3 8.3 5.8 4.6 2.6 3.3 3.1 4.7 6.3 3.2 6.3 6.7 7.7 7.9 7.9 7.0 6.6 7.0 7.4 7.3 7.4 7.3 7.1 6.7 6.5 6.3 6.2 6.2 6.0 6.1 0.11 0.07 0.11 0.11 0.09 0.06 0.10 0.13 0.11 0.12 0.15 0.09 0.08 0.06 0.12 0.08 0.13 0.09 0.09 0.08 13.9 7.5 10.9 12.1 9.5 7.4 12.0 16.8 14.5 15.4 13.9 11.0 13.2 7.8 16.8 8.4 12.7 10.3 12.8 8.8 10.7 10.8 11.1 10.8 10.2 10.5 11.3 11.6 12.0 12.2 12.1 12.2 11.8 12.2 11.9 12.0 11.9 11.9 11.8
/
Note:
CV = Coefficient of variation for control growth.
Lower CV bound determined by USEPA (1 0 1h percentile) =3.5%.
Upper CV bound determined by USEPA (9 0'h percentile) = 20%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD Is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10m" percentile) = 12%.
Upper PMSD bound determined by USEPA (90 'h percentile) = 30%.
CT = Central Tendancy (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-00S. US Environmental Protection Agency, Cincinnati, OH.
File: ppkclcr_05031 l.xlsx Or~n~
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Entered by: 3. Sumner 0,-8AQ~bPk.4Qhax, nc.Reviewed by:
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Page 1 of 5 I
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Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas PpKCICR Test Number: _
I Dilution preparation information:
Comments:
KCI Stock LNSS number:
I tus.
_i_
Stock preparation:
50 g KCI/L:
Dissolve 50 g KCI in I-L Milli-Q water.
Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:
Test information:
Organism age:
7..
toou.*
Randomizing template:
k.,i Date and times organisms O1-a2.-ý\\
i'.*
Incubator number and were born between:
shelf location:
Organism source:
_IMY.
OS,-n-A Artemia CHM number:
CA K ISO..
Drying information for weight determination:
Transfer vessel pH = "1.lt S.U.
Date / Time in oven:
o-to.1tt N ip information:
Temperature =
,A. 1..
0C Initial oven temperature:
"* (-
Average transfer volume:
Date / Time out of oven:
i.St IiS C. t'*'l*.*
Final oven temperature:
I C.
Total drying time:
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 0
itto
,L tas" J
O121L.7.1 A
A% 1L. 66
.4.....
A~*
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ito.- A, 3
S.6-1 ISOO os-(n.1%
4 OOI-So L
iAS 6
6 c*M.oCI-I*
choo 6t
- 140t, Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
1
< 20%
7-day LC50 1q*.S Average weight per initial larvae:
0.-1 NOEC C4 40o Average weight per surviving larvae:
0.94-1 0.25 mg/larvae LOEC q-S J
ChV IC25 I
& 2 Page 80 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09
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- JaEFTS,
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alTesting Saut an nc.n Page 2 of 5 Species: Pimephales Dromelas PpKCICR Test Number: l Survival and Growth Data Day Control 450 mg KCI/L 600 mg KCI/L
,,_A B
C D
E F
G H
I J
K L
0 10 1
t to I to/CIto0 1/01 1 C 1
to I
to t(0 (0
to
(
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t0 o to t0 (
2 10 to to t(o 0
to
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to
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/01 to t0
/0 t(o to 4
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10
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)*
io 1o (0
to to 0
R 0%.
, 4%
6 to 10 1
cj (0o I 103 1(0
'9 A = Pan weight (mg)
Tray color code::
PLUiM Analyst:
.t 13.4 lq 1t4.1;,4.3s4.'s i (
t4-.to*
.,5 4.41 IS,.1 4.4t, Date:
- A-~4~o~O'i B = Pan + Larvae weight (mg) 21.30 Analyste:
M U-90. 2.2.49 28 22-.1
.4'.0 21,'5 Z.4." 22*.6f 21-.21 21.05 21 50 1..1%
C = Larvae weight (mg) = B - A Hand calculated.
-441' tN 201 (,JA -S1 9-06 Analyst:
41 Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
Analyst:
01 0'
%101 0'
0" 0"
0*
0O 01 Average weight per initial Percent number of larvae (mg) reduction from control I
M)/oI 0.1&.
0.15t 7.tl Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Comments:
I I
Page 81 of 100 SOP AT21 - Exhibit AT2 1. 1, revision 04-01-09
EnvironmritairTtt~inSouionIc Page 3 of 5 Species: Pimephales promelas PpKCICR Test Number:
IS I Survival and Growth Data Day 750 min KC/L 900 mg KCI/L 1050 ml KCI/L 0
M N
0 P
Q R
S T
U V
W X
(
)0 10 10 1
10 tq 10 1(
t L.
2 tO
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/ 10
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,~o Ot
'&t Is~
5 6
9 6,,4 63 ?A I,4 7_
A A = Pan weight (mg)
Tray color code::.
&'u1A Analyst:
?A6.
10 13.O01 4-.4t,4.4 13.'-
13.,eA l4.4f t$.'
4, 13.o3 Date:
B = Pan + Larvae weight (mg)
Analyst:
enJ"*
- 2.
20A -1 2,.I*
2.*,*
i*-.90 10,5 I.5 2.2.,1, l *,
1
.tb-iJ Date:
fl..1341 C = Larvae weight (rug) = B - A Hand calculated.
S,&
"lt*O ",'l-CI'Iq 4* * '
'4.(T "Aqi 1,L*
I.A&
Analyst:
Weight per initial number of larvae (mg)
= C / Initial number of larvae Hand calculated.
- s Analyst
116gj" Ot O.
0 0
O. 0 Average weight per initial Percent number of larvae (mg) reduction
- o.
.,.7.
O,
. 2.7 from control
(%)
Comment codes: c = clear, d = dead, fg = fungus, k killed, mI missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded; I
Comments:
I Page 82 of 100 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09
L
~-
A Environmental Testing Solutions, Inc.
)
Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number PpKCaIt 231 Test dates:
May 03-10, 2011 Dunnett's MSD value:
0.0764 MSD =
Minimum Significant Difference PMSD:
- 8.
PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPAs WET Interfaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-9-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
Page 83 of 100 Organismn obraunedfin mAqnuox. Inca File: ppkclcr 05031 l.xcsx Fmtered by: J. Sumcer Reniewed by:
"ErTS SEslnmtaeslg SouinIn Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:.
5/3/2011 Test ID:
PpKCICR Sample ID:
REF-RefToxicant End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potassium chloride Sample Date:
Protocol:
FWCHR-EPA-82 1-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-mg(/L 1
2 3
4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 0.9000 1.0000 1.0000 600 0,8000 0.8000 0.9000 0.9000 750 0.9000 0.9000 0.9000 0.9000 900 0.6000 0.6000 0.6000 0.8000 1050 0.3000 0.3000 0.2000 0.2000 Transform: Arcsin Square Root Rank I-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV%
N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4
16.00 10.00 1
40
- 600 0.8500 0.8500 1.1781 1.1071 1.2490 6.954 4
10.00 10.00 6
40
- 750 0.9000 0.9000 1.2490 1.2490 1.2490 0.000 4
10.00 10.00 4
40
- 900 0.6500 0.6500 0.9413 0.8861 1.1071 11.742 4
10.00 10.00 14 40
'1050 0.2500 0.2500 0.5216 0.4636 0.5796 12.838 4
10.00 10.00 30 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.94239873 0.884 0.46929573 0.83381121 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU Steels Many-One Rank Test 450 600 519.615242 Treatments vs D-Control Trimmed Spearman-Karber Trim Level EC50 95% CL 0.0%
5,0%
10.0%
20.0%
Auto-25.0%
949.52 891.54 1011.261 orPiA9e441,10-gatox, Inc File: ppkclcr)05031 1.xlsx Entered by: J. Sumner Reviewed by: 4
SETS
,* Environmensol Testln g Stuos n Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/3/2011 Test ID:
PpKCICR Sample ID:
REF-RefToxicant End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potassium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-mg/L 1
2 3
4 D-Control 0,8530 0.8240 0.9470 0.8420 450 0.7160 0.8090 0.7590 0.8440 600 0.6290 0.6640 0.7510 0.8060 750 0.5860 0.7600 0.7730 0.6240 900 0.4340 0.4030 0.4930 0.7440 1050 0.1750 0.1880 0.1220 0.1680 Transform: Untransformed I-Tailed Isotonic Cone-mg/L Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.8665 1.0000 0.8665 0.8240 0.9470 6.345 4
0.8665 1.0000
- 450 0.7820 0.9025 0.7820 0.7160 0.8440 7.180 4
2.151 1.943 0.0764 0.7820 0.9025 600 0.7125 0,8223 0.7125 0,6290 0.8060 11.330 4
0.7125 0.8223 750 0.6858 0.7914 0.6858 0.5860 0.7730 13.806 4
0.6858 0.7914 900 0.5185 "0.5984 0.5185 0.4030 0.7440 29.874 4
0.5185 0.5984 1050 0.1633 0.1884 0.1633 0.1220 0.1880 17.593 4
0.1633 0.1884 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p> 0.01) 0.92959601 0.749 0.540073551
-0.97410495 F-Test indicates equal variances (p = 0.97) 1.04289341 47.46834564 Hypothesis Test (I-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.07635283 0.08811637 0.0142805 0.00308783 0.075051717 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL(Exp)
Skew IC05*
230.72 96.49 103.78 614.32 0.9507 ICIO 454.64 81.89 211.64 691.81
-0.0365 ICI5 548.15 86.58 396.44 894.38 0.7530 IC20 708.22 87.46 443.85 854.07
-0.1925 IiCs 782.17 55.25 523.40 935.70
-0.7858 IC40 898.74 32.73 790.71 961.75
-0.2823 ICSO 936.00 22.79 858.25 984.19
-0.3662
- indicates IC estimate less than the lowest concentration OrP,@9q§§bd9Jrn1A9uQbOx. Inc.
File: ppkclcr_050311.xlsx Entered by: J. Sumner Reviewed by:*
I T
I "3Envtmnme nt alTestlng So~utI n.s Inc.
Page 4 of 5 Species: Pimephales promelas PpKCICR Test Number:
2,k Daily Chemistry:
I I
Day Concentration Parameter Conductivity (mhos/cm) 505 CONTROL Alkalinity (mg CaCO 3/L) ek Hardness (mg CaCO 3/L)
(Temperature vs (0c)
II.
pH (S.U.)
'J.T4 II 1-',
I 02 II*.-
II.'t2.
iL3+/-3~J
~-.--
II
.5 -. dI-'-'.~-----4I-~----------
DO (mg/L) 450 mg KCI/L Conductivity (gmhos/cm)
I
/Ito J0I~lU At10 1LJ 741 1110tb 14 Temperature (0C)
S -
U 0
-iiiiIIII.*
PH (S.U.)
DO (m2IL) 600 mg KCI/L Conductivity (jmhos/cm) 13 q0 t4o15z0 V
A3 Is~.I Temperature
(°C)
U
'U pH (S.U.)
DO (mg/L) 750 mg KCI/L Conductivity (pýrnhos/cm)
I 1011%2 1(540 Temperature (0C)
I-DO mj 900 mg KCI/L Conductivity Temperature "44.1 (c),
pH (S.U.)
-q'(91 I'sk I'm qlt__, -ý hn f,., -/I I
ILIUtgt.,
1050 mg KCI/L Conductivity (gmhos/cm)
I
?AID"71 U
Temperature (0c)
STOCK Conductivity
___________(jmhos/cm)
~
ot Initial II Page 86 of 100 SOP AT21 - Exhibit AT21.1, revision 04-01-09
F" S
3
ý) EnvimnMentaiTesting Sollnk Ionc..
Page 5 of 5 Species: Pimephales promelas PpKCICR Test Number:
I Day 3
4 5
6
___________Analyst Concentration Parameter Conductivity (Amhos/cm).3z CONTROL Alkalinity a
k, (mg CaCO3/L)
Hardness
_(mg CaCO 3/L)
Temperature pH(".,
".,)
I.-i$
l-..
iA.!
511"
-M -3q "
" do DO (mg/L)
.1.
.5 60 g CI m'*
i
%]
Conductivity
- 73 7*
l 450 mg KCIL Conductivity Temperature "M.
U-1
_pH (0C)
_:1.9_.
"-W DO (mgIL)
-1 9-.
900 mg KCIL Conductivity 45 (iL mhos/cm)
Temperature
".4 V.
i"-'
.3
_(C)
-pH (S.U.)
DO (mg/L) 750 mg KCI/L Conductivity (Amhos/cm)_t Temperature "L.4.r pH
(°C) 9 I
Initial Final Initial Final I nitial Final Initial 1 Final I
SOP AT21 - Exhibit AT2 1.1, revision 04-01-09 Page 87 of 100
O
- D Ceriodaphnia dubia Chronic Reference Toxicant. Control Chart Environmental Testing Solutions, Inc.
1.14 1.12 1.10 1.08 1.06 1.04 1.02 2.5 I
C on o
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USEPA Control Limits (+ 2 Standard Deviations)
,a°................'....................................
K I
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I N
N N
2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 S
I II I
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th th
- USEPA Warning and Control Limits (75 and 90 Percentile CVs)
I I I
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I Laboratory Warning and ControlLimits (10 and 25 Percentile CVs) 1.0 0.9 0.8 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 +/- SA.10 or SA.75)
......... Control Limits (mean IC25 +/- S A25, SA90, or 2 Standard Deviations)
Page 88 of 100 Graphs generated from associated excel spreadsheet.
Excel spreadsheet mrtered by: J. Sumner Reviewed by:
Environmental Testing Solutions, Inc.
Test number Test date 7-day JC2s.
CT (g/L NaCI)
(g/L NaCI)
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 12=08-09 01-05-10 02-02-10 03-02-10 04-06-10 05-04-10 06-08-10 07-13-10 08-03-10 09-14-10 10-05-10 10-05-10 11-02-10 12-07-10 01-18-11 02-08-1I 03-08-1 1 04-05-11 04-05-Il 05-03-11 1.05 1.08 1.07 1.08 1.05 1.09 1.07 1.07 1.06 1.07 1.08 1.08 1.09 1.06 1.06 1.06 1.05 1.06 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07' 1.07 1.07 1.07 1.07 1.07 1.07 State and USEPA S
Control Limits S.:o CT-2S CT+2S 0.02 1.02 1.11 0.09 0.02 1.04 1.10 0.09 0.01 1.04
. 1.10 0.09 0.01 1.04 1.10 0.09 0.02 1.04 1.11 0.09 0.02 1.04 1.10 0.09 0.01 1.04 1.10 0.09 0.01 1.04 1.10 0.09 0.01 1.04 1.10 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.10 0.09.
0.01 1.05 1.10 0.09 0.01 1.04 1.10 0.09 0.01 1.04 1.10 0.09 0.01 1.04 1.09 0.09 0.01 1.05 1.09 0.09 Laboratory Warning Limits CT - SAJO CT + SAl.
Laboratory SA.25 Control Limits CT - SA-2 CT + SA2u USEPA SA.s" Warning Limits CT - SAMs CT + SA.7s USEPA S"
Control Limits CT-Sse CT + S,.
0.98 0.98 0.99 0.98 0.99 0.99 0.99 0.98 0.98 0.99 0.99 0.99 0.99 0.99 0.99 0.98 0.98 0.98 0.98 1:15 1.16 1.16 1.15 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.1.8 0.18 0.18 0.89 0-89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 1.25 1.25 1.25 1.25 1.26 1.25 1.25 1.25 1.25 1.25 1.25 1.26 1.25 1.25 1.25 1.25 1.25 1.25 1.25 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59.
0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 1.55 1.55 1.55 1.55 1.56 1.55 1.55 1.55 1.55 1.55 1.55 1.56 1.56 1.55 1.55 1.55 1.55 1.55 1.55 0.66 0.66 0.66 0.66 0.67 0.66 0.66 0.66 0.66 0.66 0.66 0.67 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41
.0.41 0.41 0.41 0.41 0.41 0.41 0.41 1.73 1.73 1.74 1.73 1.74 1.74 1.74 1.73 1.73 1.73 1.74 1.74 1.74 1.74 1.74 1.73 1.73 "1.73 1.73 Cv 0.02 0.02 0.01 0.01 0.02 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Nole:
7-d ICm = 7-day 25% inhibition concentration. An estimation of the concentration ofl'odium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT ý Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
SA.jO = Standard deviation corresponding to the 10"' percentile CV. (SA& =0.08)
SA.zs = Standard deviation corresponding to the 25" percentile CV. (SA.2s =0.17)
USEPA Control and Warning Limits SA.S = Standard deviation corresponding to the 75"' percentile CV. (SAMS
= 0.45)
SA9o = Standard deviation corresponding to the 90"h percentile CV. (SA.9o = 0.62)
CV = Coefficient of variation of the IC25 values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.
Page 89 of 100 Fie: d~aCC b5y Is-l H
by--.Sutn
EnT Environmental Testing Solutions. Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 00 0
- 00 I-0I.'
00Q T
40 35 30 25
.20 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
- I i
i I i
i i
i i
I I i ti i
i 40 30
- o 20
- Q10 50
.....I I
I I
I I
I I..
North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
40 30 I
I
_ I
_-I I
I I
I I
I I
I-USEPA Upper PMSD Bound (90th percentile < 47.0%)
I I
I I.
20 10 A1 V
k,.V% sm S"o l'o I.v% S'NN *"liN tNý S"ilN SAN
%-511 0-11 NNA ýO %VV %IVA 0.", 0-11. 011, Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Page 90 of 100 Graphs generated from associated excel spreadsheet.
Excel spreadsheet ejter d by: J. Sumner Reviewed by: 7
0 SEnvironmental Testing Solutions, Inc.
Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data est TControl number T
Survival Control Mean Reproduction
(%)
(offspring/female) 1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 12-08-09 01-05-10 02-02-10 03-02-10 04-06-10 05-04-10 06-08-10 07-13-10 08-03-10 09-14-10 10-05-10 10-05-10 11-02-10 12-07-10 01-18-11 02-08-11 03-08-11 04-05-11 04-05-11 05-03-11 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 35.2 31.1 31.9 31.0 32.7 31.0 33.1 31.6 28.2 32.9 30.2 30.8 30.5 32.2 31.9 30.7 32.6 32.8 33.0 30.9 CT for Control Mean Reproduction (offspring/female) 33.2 32.7 32.3 32.4 32.2 32.3 32.2 31.8 31.9 31.7 31.6 31.6 31.6 31.6 31.6 31.6 31.7 31.8 31.7 CV CT for Control
(%)
Reproduction Cy (%)
MSD PMSD
(%)
for PMSD (%)
CT 4.6 5.8 3.5 6.3 6.3 4.6 6.6 4.8 4.7 8.7 7.6 4.0 4.9 4.8 6.3 6.9 4.8 5.9 4.5 6.7 5.2 4.6 5.0 5.3 5.2 5.4 5.3 5.2 5.6 5.7 5.6 5.6 5.5 5.6 5.6 5.6 5.6 5.6 5.6 2.0 2.3 2.6 2.4 2.4 2.3 2.4 2.3 1.8 3.0 2.0 2.2 2.0 2.0 2.3 1.8 2.0 2.2 1.9 2.5 5.8 7.3 8.3 7.7 7'4 7.4 7.2 7.2 6.2 9.2 6.6 7.2 6.6 6.2 7.1 5.7 6.0 6.8 5.7 8.1 6.6 7.1 7.3 7.3 7.3 7.3 7.3 7.2 7.4 7.3 7.3 7.2 7.2 7.2 7.1 7.0 7.0 6.9 7.0 Note:
CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (10I' percentile) = 8.9%.
Upper CV bound determined by USEPA (90&' percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90th percentile) = 47%.
CT Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICR_05031 l.xlsx Entered by: J. Stunner Page 91 of 100 Reviewed by:_4 1
Fr Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCICR #:
11O Dilution preparation information:
Comments:
NaCI Stock INSS number:
UIWS_
1131 Stock preparation:
100 g NaCI/L:
Dissolve 50 g NaCI in 500 mL Milli-Q water.
Dilution brep (rg/L) 600 800 1000 1200 1400 Stock volume (mLr 9
12 15 18' 21 N
Diluent volume rmL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 1
Test organism source information:
Test information:
Organism age:
< 24-hours old Randomizing template color:
(l.k Date and times organisms were born 0*O%4j% -n'*vri VLOG Incubator number and shelf between:
location:
Culture board:
A Replicate number:
1 21 3 1 4 1 s 1 6 1 7 0 8 1 9 ito YWT batch:
Culture board cup number:
S "1
1 SI lq
_A 1I. LtI
_______S__
I Transfer vessel information:
PH = 2_
S.U.
Temperature =
.L 'C Selenastrum batch:
O4 z."il Average transfer volume (mL):
1 A.
4 0,4.M-Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0
Qf-1
'S 01TL,60 A
- NO 1
eI6q-I I S t.
QS l1 F 2
s.-1. IIIus 0 d 6s____
I70 n,.a-S. I 5
_______1 1200 CG L I-I-A 6
.15
'1 Control information:
Acceptance criteria Summary of test endpoints:
% of Male Adults:
67,
< 20%
7-day LC50 Won
% Adults having 3r Broods:
fo?
a 80%
NOEC
&00
% Mortality:
0*,
520%
LOEC
/000 Mean Offspring/Female:
15.0 offspring/female ChV gig, I
%CV:
<40.0 %
IC2s5 0,K(0 II Page 92 of 100 SOP AT 14 - Exhibit AT 14.1, revision 04-01-09
3
,. E vbrnm Tn t11Sotfln.Tlo s I Ac.
Page 2 of 6 Species:. Ceriodaphnia dubia CONTROL CdNaCICR :
VZ Survival and Reproduction. Data Replicate number
- Day, 1
2 3
4 5
6 7
8 9
10 I
Young produced Q
0 C).
C,._
0 0
0 0
Adult mortality
\\
Adult mortality I
j 3
Young produced I.~
0 0
0 0
I 0 C
Adult mortality
\\--
+/-
I
\\--
'" I'-
\\--
4 Young produced
&4.
I ]
'\\4 A.
Adult mortality t-L~
L-Adult mortality L..
6
[ Young produced 0
==
0 0
0 0
Adult mortality.
t......
7 Young produced 1
1 b 14*t, I to I
I Total young produced S.
-6 I2 3 L
- 5. o
- 32.
7-- 0 Final Adult Mortality s...
W X for 3rV Broods y_
1
>52
>1 Mote: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
S% Mortality:
I 01.
Mean Offsprin emale:
600 m NaC IL Survival and Reproduction Data Replicate number Day 1
1 2
3 4
5 6
7 8
9 10 I
Young produced 0-()
0 i(0 0f0 I
Adult mortality
\\-
\\
I--..
k-_
2 Young produced 0
0 0
0 0101010 0
Adult mortality
[
L.
L
. L 3
Young produced 0
IQ 0 0
0 10 0
Adult mortality
[
1_. I L4_.
4 Young produced
[ c 4"
Adult mortality F-C
_ I, L.
jLJL...
5 Young produced 1,
[.j*
iL I
i I j ""
I I \\
IL JL Adult mortality jI-L U~_
6 Young produced 100 C I
C) n (o) o L
[Adult mortality C
T' j J
L.-
E U- -
JL 7~~ Young produced t
V!5 I
I%
tip 1\\
Cb ] \\
I Total young produced 2A zl.
'51 I
'-i,
,3 Final Adult Mortality k...
.L Mote: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry ovei (offspring carried over with adult during transfer).
I I
I I
I I
I Concentration:
% Mortality:
07.
Mean OffsDrina/Female:
I 4n.
% Reduction from Control: 1OW.
1 SOP AT 14 - Exhibit AT 14. 1, revision 04-01-09 I
Page 93 of 100
))_
Page 3 of 6 Species: Ceriodaphnia dubia 800 m NaCI/L CdNaCICR #:
t?.c)
Survival and Reproduction Data Replicate number Day 1 1 2
3 4
5 6
7 8
9 10 I
Young produced Q
... c 6
O 1 0 0
0 0
Adult mortality Young produced 0 I 0
Adult mortality L
L._
4 Young produced
., I-Adult mortality t4I 5
Young produced iL2 10 o
to 10 7 Z.
0 10 10D Adult mortality
,..L_._
Adult mortality V_
t_.'-
7 j Young produced I' S IS%
\\1 t )
I%.
it. I*
1 4 I Sq Total young produced 3*
.*b 3 1 7"]
1Final Adult Mortality K
-1 Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
07, Mean Offspring/Female:
21-1
% Reduction from Control:
3.27.
1000 mg NaCI/L Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 Adult mortality Adult mortality=-=.
j L
3 Young produced Li Adult mortality
[
L Young produced fl fl[Q
- otO1, Adult mortillity Adult mortality IL1 LJ L 6
Young produced
_0I0 Adult mortality I
t...
- J... I
=
7 Young produced t
JL IS
...1*".
Total young produced f_0==.
Total y pdu c
vi MortaVity.
3b 1-2-1 1Final Adult Mortality
=
-j i
l:
I Note: Adult mortality (L = live, D = dead), SlB = split brood (single brood split between two days), UU = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
7 0.
Mean Offspring/Female:
2t.0
% Reduction from Control:
'.4 7.
SOP AT14 - Exhibit AT14.1, revision 04-01-09 Page 94 of 100
I I
I I
I I
-I II.
I I
I I
I Page 4 of 6 Species: Ceriodaphnia dubia 1200 ma NaCI/L CdNaCICR #:
1rc:3 Survival and Reproduction Data Replicate number Day
_1 2
3 4
5 6 1 7 8
9 10 Young produced c-C
)
C Adult mortality l -
1 Young produced C) o C>
p c'
iC Adult mortality U-
[
1 -
].
3 Young produced
_ b I Adult mortality V-L U
4 Young produced p
0.I Q. I c - I
. I..
I 1
0
"" Adult mortality
_ 4
- I L
5 Young produced Ik 3*__
It
... V Adult mortality L.L U JU
[
6 Young produced
- 0.
0AI Cl j
0I 0I Adult mortality I
1~
7
[Young produced 0*1 A~
12 Q
0 Total young produced 1-I
-1 1 Final Adult Mortality l *I j
I L-i...
Note: Adult mortality (L = live, D dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
__Concentration:_
[
IConcentration:
% Mortalit:
W.
[ Mean Offspring/Female:
I &."
% Reduction from Control:
51.01.
1400 mg NaCI/L Survival and Reproduction Data Replicate number Day 2
3 4
5 6
7 8
9 10 o
Young produced
(*
[ J C
)
0
(
(
Adult mortality 2
Young produced 10 50 0
0 0
0 0
0 0
0' Adult mortality t-V...
L,-
33 Yon prdue c
o 0 0
Adult mortality J.
L..
L-L
(..
Adult mortaffity V,
C Q-
.=:~
I
-I
Young produced 0
C L
0 7-0 1 0 Adult mortality L
L L-L 6
Young produced I.
0 0
0 Adult mortality U-J J
I
£ U. I-7 -
Young proded cc
-k Co
%4 7
Total young produced
- 0.
1 T4!
21 6
Final Adult Mortality C___
L
=
=
Note: Adult mortality (L = live, D dead), SB split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer).
I I
Concentration:
% Mortality:
1.
Mean Offspring/Female:
4L,3
% Reduction from Control:
A
.17-I Page 95 of 100 SOP AT 14 - Exhibit ATI 4.1, revision 04-01-09
o
- 3)
Environmental Testing Solution s, Inc.
Verification of Ceriodaphnia Reproduction Totals Control Replicate number Total Day 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 3
01-2 0 10 03 01 02 12 02 02 0
01 4
4 4
3 5
4 4
6 4
4 3
41 5
12 12 I0 13 II 12 12 12
,12 10 116 6
0 0
'0 0
.0 0
0 0
0 0
0 7
16 16 13 14 16 16 15 16 13 17 152 Total 32 32 26 32 31 32 33 32 29 30 309 600 mg NaCM/L Day Replicate number Total 1
2 1 3 4 1 5 1 6 7
1 8 1 9 10 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
.0 0
0 0
0 0
3 0
0 0
0 0
0 0 0 0
0 0
0 4
5 3
3 4
3 4
5 4
4 4
39 5
13 10 12 12 10 12 11 11 I
12' 114 6
0
,0 0
0 0
0 0
0 0
0 0
7 18 15 15 16 13 17 15 15 16 15 155.
Total 36 28 30 32 26 33 31 30 31 31 308 800 iug NaCI/L Replicate number Total D
1 2 13 4
5 16 7 18 19 10 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
3 4
4 5
4 4
4 3
3 38 5
12 10 10 II 10 12 10 10 10 13 108 0 0 0
0 0
0 0
0 0
0 7
15 15 17 15 13 16 16 17 14 15 153 Total 31 28 31 30 28 32 30 31 27 31 299 1000 mg NaC/L-Re plicate number Day 1
2 3
4 5
6 7
8 9
10 Total 1
0 0
0 1 0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 3
3 4
4 3
3 3
5 3
3 34 5
10 12 9
12 10 10 9
!11 10 10 103 6
0 0
000 0
0 0
0 0
0 7
1 15 13 13 15 14 14 14 15 14 143 Total 29 30 126 29 28 27 26 30 28 27 280 1200 mg NaCI/L Replicate number Total Day 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 1 0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 2
3 3
4 3
1 3
2 2
4 27 5
8 5
5 7
4 6
4 5
4 7
55 6
0 0
0 0
0 0
0 0
0 0
0 7
9 8
6 3
5 0
6 4
3 7
51 Total 19 16 14 14 12 7
13 11 9
18 133 1400 mg NaCI/L Day Re plicate number Total 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
1 0
0 I
0 0
0 2
0 0
4 5
0 2
1 0
2 2
0 0
1 3
II 6
3 0
0 0
0 0
3 0
0 0
6 7
4 1
0 4
2 2
I 5
0 3
22 Total 8
3 I
5 4
4 4
7 I
6 43 Page 96 of 100 File: CdNaCICR 050311.xlsx Entered by: J. Sumner Reviewed by:
O
E EnvironmentalITesting Solutions, Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
Test dates:
CdNaCICR #120 May 03-10,2011 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variation (%)
control (%)
Control 32 32 26 32 31 32 33 32 29 30 100 30.9 6.7 Not applicable 600 36 28 30 32 26 33 31 30 31 31 100 30.8 8.8 0.3 S00 31 28 31 30 28 32 30 31 27 31!
100 29.9 5.6 3.2 1000 29 30 26 29 28 27 26 30 28 27 100 28.0 5.3 9.4 1200 19 16 14 14 12 7
13 11 9
18 100 13.3 28.4 57.0 1400 8
3 I
5 4
4 4
7 1
6 100 4.3 53.8 86.1 Dunnett's MSD value:
PMSD:
2.511 8.1 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90th percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001b).
USEPA. 2001 a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82 I-B-01-004 and EPA-82 I -B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
File: CdNaCICR_050311.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.
Spreadsheet entered by: J. Surpner Page 97 of 100 Reviewed by:
S 0
0VETS
~
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/3/2011 Test ID:
CdNaCICR Sample ID:
REF-Ref Toxicant End Date:
5/10/2011 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
NACL-Sodium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10 D-Control 32.000 32.000 26.000 32.000 31.000 32.000 33.000 32.000 29.000 30.000 600 36.000 28.000 30.000 32.000 26.000 33.000 31.000 30.000 31.000 31.000 800 31.000 28.000 31.000 30.000 28.000 32.000 30.000 31.000 27.000 31.000 1000 29.000 30.000 26.000 29.000 28.000 27.000 26.000 30.000 28.000 27.000 1200 19.000 16.000 14.000 14.000 12.000 7.000 13.000 11.000 9.000 18.000 1400 8.000 3.000 1.000 5.000 4.000 4.000 4.000 7.000 1.000 6.000 Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 30.900 1.0000 30.900 26.000 33.000 6.728 10 30.900 1.0000 600 30.800 0.9968 30.800 26.000 36.000 8.766 10 0.091 2.287 2.511 30.800 0.9968 800 29.900 0.9676 29.900 27.000 32.000 5.563 10 0.911 2.287 2.511 29.900 0.9676
- 1000 28.000 0.9061 28.000 26.000 30.000 5.324 10 2.641 2.287 2.511 28.000 0.9061
- 1200 13.300 0.4304 13.300 7.000 19.000 28.366 10 16.027 2.287 2.511 13.300 0.4304
- 1400 4.300 0.1392 4.300 1.000 8.000 53.763 10 24.223 2.287 2.511 4.300 0.1392 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.82175648 1.035
-0.1981616 0.6557231 I Bartlett's Test indicates equal variances (p = 0.07) 10.1990776 15.0863171 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 1000 894.427191 2.51109516 0.08126522 1279.06667 6.02962963 3.3E-34 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL Skew IC05 ICI0 IC15 IC20 857.368421 101.848477 536.115476 1000.42718
-1.5395 1002.58503 37.7430109 902.799145 1019.00076
-1.1222 1023.60544 9.16420366 1004.92346 1039.04671 0.1840 1044.62585 8.95991641 1027.21063 1060.90996 0.1797 lIC25 1065.64626 9.15526908 1047.78147 1082.77122 0.19211 IC40 1128.70748 11.7423916 1107.58171 1149.34211 0.4319 IC50 1170.7483 14.442622 1144.89854 1199.51128 0.5658 File: CdNaCICR 050311.xlsx Entered by: J. Sumner Reviewed by:
Page 98 of 100
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Page 5 of 6 Species: Ceriodaphnia dubia CdNaCICR #:
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(°C)
STOCK Conductivity 10 (Amhos/cm) 10 /o00 Initial.11 Final Initial 11 Final I Initial Final I
SOP AT14 - Exhibit AT14.i, revision 04-01-09 Page 99 of 100
Species: Ceriodaphnia dubia Page 6 of 6 CdNaCICR 1: 10..
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Initial 11 Final Initial 11 Final I Initial 1 Final I Initial Final I
I Page 100 of 100 SOP AT14 - Exhibit AT14.1, revision 04-01-09
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 200 (INTEROFFICE OPS-jN-SQN)
SODDY - DAISY. TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
Form Approved.
OMB No. 2040-0004 TN0026450 II L 103 G F-FINAL PERMIT NUMBER IDISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND MANITAIrINr. DPiD~fnf I
EFFLUENT FromI YEAR 05 01
-DAY To 11 M05 I K NO DISCHARGE NOTE: Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE PARAMETER QUANTITY OR LOADING EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7
8 12 0
13/31 GRAB MEASUREMENT 00400 1
0 PERMIT 6
9 SU THREE/
GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 13 14 19 0
2 / 31 GRAB MEASUREMENT 00530 1
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GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE
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<6 19 0
2 / 31 GRAB MEASUREMENT 00556 1
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GRAB EFFLUENT GROSS REQUIREMENT,
MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 0.985 1.303 03 0
31 /31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 PERMIT Req. Mon.
Req. Mon MGD SEE RCORDR EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX PERMIT SAMPLE MEASUREMENT PERMIT.
REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my r-.1*"..
TELEPHONE DATE SDlirection or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vic&-#r*ident information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties-for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE ncluding the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE OPS-5N-SON)
SODDY - DAISY. TN 37384 Facilitv TVA - SEOUOYAH _NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR).
(SUBR 01)
TN0026450 I
110 G I F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER SMONITORING, PERIOD IEFFLUENT Form Approved.
OMB No. 2040-0004 I Yro A I MO TODAY Y
From l 111 T05 01 1 TO I11 I05 I31I ATTN: Stephanie A. Howard PARAMETER NO DISCHARGE Fjx...
NOTE: Read instructions before completing this form.
QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS TEMPERATURE, WATER DEG.
SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 1
0 PERMIT REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS TEMPERATURE, WATER DEG.
SAMPLE CENTIGRADE MEASUREMENT 04 00010 Z
0 PERMIT 30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. &
SAMPLE UPSTRM DEG.C MEASUREMENT 04 00016 1
0 PERMIT 5
DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE 03***
TREATMENT PLANT MEASUREMENT 03 50050 1
0 PERMIT Req. Mon.
MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1
0 PERMIT 0.1 0.1 MGIL Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE 04***
CHANGE MEASUREMENT 04 82234 1
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DEG C CONTIN CALCTD REQUIREMENT DIYM EFFLUENT GROSS VALUE DAILY MX uous SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or e
persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vic'4sident information, the information submitted is, to the best of my knowledge and belief, true, accurate, y
423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I
including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
A ZRR COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address p.O.BX 2000 (INTEROFFICE OPS-5N-SQN)
SODDY - DAISYTN M
7384 Facility TA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
PERMIT NUMBER 1 DISCHARGE NUMBER o
MONmITRING PE:RIOD f
-YEAR I MO IDAY I YA-IDY FromL 11 1 05 01 o 1 To [ 1 I ot s 1 31 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0 F - FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE NOTE: Read instructions before completing this form.
004 ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STi CERIOD TRP3B EFFLUE IC25 STA PIMEPH TRP6C EFFLUE ATRE 7DAY CHR SAMPLE 23 APHNIA MEASUREMENT 1
0 0
PERMIT 43.2 PERCENT SEMI COMPOS NT GROSS VALUE REQUIREMENT MINIMUM ANNUAL ATRE 7DAY CHR SAMPLE 23 ALES MEASUREMENT 23 1
0 0
PERMIT 43.2 PERCENT SEMI COMPOS REQUIREMENT NT GROSS VALUE MINIMUM
_____ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT F
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+/-
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+
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my Y
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TELEPHONE DATE Mairection or supervision in accordance with a system designed to assure that qualified personnel Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice dent information, the information submitted is, to the best of my knowledge and belief, true, accurate, y
_423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE OPS-5N-SON)
SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAHNUCLEAR PLANT Locatio HAMILTON COUN-TY.
ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 t
118G F-FINAL PERMIT NUMBER DISCHARGE NUMBERI WASTEWATER & STORM WATER Form Approved.
OMB No. 2040-0004 amf~Ifn~rfr~l I
EFFLUENT MILMLLVELULNU Itfr-EUMU Fromi1 I
I YEAR 1
TO I
1 28y NO DISCHARGE
--1
- NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE I
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT 19 00300 1
0 PERMIT 2
MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1
0 PERMIT 100 MGIL TWICE/
GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 25 00545 1
0 PERMIT MUL ONCE/
GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03*
TREATMENT PLANT MEASUREMENT 03 50050 1
0 PERMIT Req. Mon.
Req. Mon.
MGD
- ONCE/
ESTIMA REQUIREMENT EFFLUENT GROSS MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel t
Michael D. Skaggs properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site84Vice01 11s06nt information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 11 06 07 Sequoyah Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED ORPRINTED OFFICER____OR__AUTHORIZED_____A__ENT__AREA__
NUMBER____YEAR__MO__DAY_
CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3/99) EPA Form 3320-1 (REV 3199)
Previous editions maybe used Page 1 of 1 Previous editions may be used Page 1 of 1