ML102000308
| ML102000308 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/12/2010 |
| From: | Church C Tennessee Valley Authority |
| To: | Cromer P Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML102000308 (114) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy. Tennessee 37384-2000 July 12, 2010 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JUNE 2010 AND CORRECTION TO DISCHARGE MONITORING REPORT FOR MAY 2010 Enclosed is the June 2010 Discharge Monitoring Report for Sequoyah Nuclear Plant. Also, enclosed is the correction to the Discharge Number 101 T, Biomonitoring for Outfall 101 for May 2010. If you have any questions or need additional information, please contact Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
/ certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true; accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
Sincerely, Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
T0026450 1
101 G F-FINAL NPERMITNUMBER I D11SCHARGE NUMBER DIFFUSER DISCHARGE Ma NIT.RING_.EFFLUENT Form Approved.
OMB No. 2040-0004 F ErAo MQ DAY I From 110 i06 01I To Io o 1 i
ATTN: Stephanie A. Howard NO DISCHARGE F___
NOTE: Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the id6 e
n information, the information submitted is, to the best of my knowledge and belief, true, equoyail Site Vi e
423 843-7001 10 07 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
_____O________E___
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments* e, No closed mode operation. The following information is included in an attachment: CCW data EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.
CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/16/2010 @ 1323
<0.10 mg/f 06/18/2010 @ 0140 KMF EPH
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN065 10 F -FINAL PERMIT NUM13ER DISCHARGE NUMBERJ DIFFUSER DISCHARGE
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NOTE: Read instructions before con PARAMETER CHLORINE, TOTAL RESIDUAL 50060 1
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my
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direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or
/1 persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED_ ORPRINTED_
information, including the possibility of line and imprisonment for knowing violations.
OFFICER ORAUTHORIZED AGENT TYPED ORPRINTED_____________________________
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments het The following injections occurred: 1. Floguard MS6236 (max. calc. conc. was 0.032mg/L--limit 0.2mg/L) 2. Biodetergent 73551 (max. calc. conc. was 0.017mg/L--limit 2.Dmg/L) 3. Spectrus CT1300 (max. calc. conc. was 0.04mg/L--limit 0.050mg/L) 4. Spectrus CT 1300(Iow detection level analytical method was <0.05mg/L--limit 0.050mg/L)
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SQN)
S-ODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TNO02640 I 1 10 PERMIT NUMBER DISCHARGE N R
FrMoNITm RINLPE YEAR Q
Om I YER MO Q AY From l 10 I04 01 1 To 1 10 1 O6r-3111 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL DIFFUSER DISCHARGE EFFLUENT
- NO DISCHARGE NOTE: Read instructions before completing this form.
.1.
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertifyunt Christopher R. Church Sequoyah Site Vice President properly ga persons wh information, accurate, ai informatior der penalty of law that this document and all attachments were prepared under my supervision in accordance with a system designed to assure that qualified personnel ther and evaluate the information submitted. Based on my inquiry of the person or o manage the system, or those persons directly responsible for gathering the the information submitted is, to the best of my knowledge and belief, true, nd complete. I am aware that there are significant penalties for submitting false including the possibility of fine and imprisonment for knowing violations.
4tfUoyah SigeVc rsdn TELEPHONE DATE 1 423 843-7001 10 07 09 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED AREA I NUMBER YEAR MO DAYI I
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het Boron was sampled on 4/14/2010.
EPA orm33201 (EV 399) Prevouseditonsmay e ued 1'ag 1 OT EPA Form 3320-1 (REV 3199)
Previous editions may be used P'age 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 FacilitV TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ArrN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fot DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) oM 0026450 101 T F-FINAL
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PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1
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EFFLUENT GROSS VALUE NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my
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- TELEPHONE DATE Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice esident 423 843-7001 10 07 09 information, the information submitted is, to the best of my knowledge and belief, true, 423 8
0 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachlments hef Toxicity was not sampled in June 2010.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT LocatIo HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES). MAJOR DISCHARGE MONITORING REPORT (DMR)
Form Approved.
(SUBR 01)
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'REQUIREMENT SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Centify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Rdrection or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 07 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
TYPEDORPRINTED
_information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
DE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
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OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
__DE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments let No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-S-N)
SODDY - DAISY. TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY_
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 110 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER A. =*RI Nr,,PFRIQQ EFFLUENT Form Approved.
OMB No. 2040-0004 From oo
- DA NO DISCHARGE XX NOTE: Read instructions before completinq this form.
ATTN: Stephanie A. Howard NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personn Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het No Discharge this Period EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1 EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT "
Address P.O. BOX 2000 (INTEROFFICE SB-2A-_S-N)
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PERMIT NUMBER I DISCHARGE NUMBER FrmNITRING PEROD From 1
10 1
06 301_*T MAJOR (SUBR 01)
F - FINAL RECYCLED COOLING WATER EFFLUENT Form Approved.
OMB No. 2040-0004 ATTN: Stephanie A. Howard NO DISCHARGE XX NOTE: Read instructions before completinq this form.
SAMPLE TYPE PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. IFREQUENCY EX OF ANALYSIS SAMPLE TYPE IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1
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i information, the information subm itted is, to the best of m y knowledge and belief, true, 423_
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information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED IOE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachtments he, No Discharge this Period EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address _P*.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
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(SUBR 01)
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NOTE: Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
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OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments hef Operations performs visual inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 ot 1 EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 I117 G
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OMB No. 2040-0004 MONITORING PFRIOD
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law t hat this document a nd all a ttachm en ts were prepared u nd ermy S,
Z n*
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person of persons who manage the system, or those persons directly responsible for gathering the "eu a
Sit Vic Pei nt information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 07 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED CODM COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al l
attachments her Operations performs visual inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 FacilitY TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040-0004 TN0026450 t1 118 G F-FINAL PEIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER TEFFLUENT From [jolo61o-ll To l 0
13NO DISCHARGE
{i NOTE: Read instructions before comoletino this form.
ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING
[
QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
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REQUIREMENT K
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cerify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE
.irection or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah S te Vice President information, the information submitted is to the best of my knowledge and belief, true, 423 843-7001 10
.07 09 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTED
_information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments h7ef The Dredge Pond (Outfall 118) was discharged June 9-11, 2010.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For DISCHARGE MONITORING REPORT (DMR) om (SUBR 01)
TNZ026450 101lT F -FINAL PERMT NUMB1ER DISCHARGE NU BIOMONITORING FOR OUTFALL 101 LQW MNIRRING P:ERIOD EFFLUENT rm Approved.
lB No. 2040-0004
-1 From 1 O0 1
01 o To*
- NO DISCHARGE
[:::]...
NOTE: Read instructions before completinq this form.
PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1
0 0
EFFLUENT GROSS VALUE-t IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1
0 0
EFFLUENT GROSS VALUE i
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Christopher R. Church I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTI, information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT TELEPHONE DATE 843-7001 10 07 09 423 Sequoyah Site Vice President TYPEn OR PRINTEDl VE AREA NUMBER YEAR MO I DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments her Toxicity was sampled May 9-14, 2010. Report is attached.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1 EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
June 10, 2010 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2010 Per your request, I am only submitting an electronic copy of the subject report.
The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected May 9-14, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Sherrard, R. M., PSC 1X-C Files, OE&R, CEB 1B-M SQN May 2010M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report-Date: June 10, 2010
- 1. Facility I Discharger: Sequoyah Nuclear Plant / TVA
- 2. County /State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
- 7.
1Q10: 3,491
- 8. OutfallTested: 101
- 9. Dates Sampled: May 9-14, 2010
- 10. Average Flow on Days Sampled (MGD): 1625.40, 1639.25,1649.92
- 11. Pertinent Site Conditions: Spectrus CT 1300 (non-oxidizing biocide used for mollusk control) was injected into the ERCW B train beginning on 5/11/10 @ 1145 through 5/14/109 @ 1520.
- 12. Test Dates: May 11-18, 2010
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. ConcentrationsTested (%)" Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: IC25 = 45.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: IC25 > 100%
Ceriodaphnia dubia: IC25 > 100%
Page 1 of 99
UV treated Outfall 101: Pimephalespromelas: IC25 > 100%
- 18. Facility
Contact:
Ann Hurt Phone #: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
jim Sumner Phone #: (828)350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected May 9-14, 2010, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >
100 percent.
Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period.
Minnow growth in the intake samples was significantly lower than the control.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 2 of 99
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM-DD-YY),
(MM-DD-YY)
Arrival Initial (MM-DD-YY)
Sample Time (ET)
Time (ET)
'Temp.'
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 101 05-09-10 0701 to 05-10-10 1445 1.7, 2.1
<0.10 05-11-10 1329 101 05-10-10 0601
'05-12-10 1230 05-09-10 0828 to 05-11-10 1329 Intake 05-10-10 0728 05-10-10 1445 0.9
<0.10 05-11-10 129 05-11-10 0656o 05-12-10 1230 101 05-11-10 0656 to 05-12-10 1425 1.3, 1.4
<0.10 05-13-10 1233 10k 05-12-10 0556 0
05-14-10 1232 05-11-10 0713 to 05-13-10 1233 Intake 05-12-10.0613 05-12-10 1425 0.8
<.0.10 0511023 05-13-10 0653 to 05-15-10 1233 101 05-14-10 0553 05-14-10 1425 2.2, 1.4
<0.10 05-16-10 1231 05-17-10 1234 05-15-10 1233 Intake 05-13-10 0711 to 05-14-10 1425 2.2
<0.10 05-16-10 1231 05-14-10 0611 05-17-10 1234
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 _ 1.0LC) in a warm water bath.
Aliduots of Outfall 101 and Intake samDles were UV-treated throuah a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page 3 of 99
Pimephales promelas Test Organisms:
Ceriodaphnia dubia In-house Cultures
- 1. Source:
Aquatox, Inc.
- 2. Age:
19.87 - 20.13 hours1.50463e-4 days <br />0.00361 hours <br />2.149471e-5 weeks <br />4.9465e-6 months <br /> old
<24-hours old Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8. Test Temperature: Outfall 101:
10 1
05-11-10 1152 ET 05-11-10 1208 ET 05-18-10 1120 ET 05-18-10 1137 ET Mean = 24.7°C (24.2 - 25.00C) 05-11-10 1329 ET 05-18-10 1250 ET Mean = 24.90C (24.6 - 25.30C)
Test Temperature: UV-Treated Outfall 101:
Mean = 24.80C (24.2 - 25.20C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
Page 4 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 11 - 18, 2010 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2J.
3 [ 4 5
6 I
7 Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(
TEfluet)
(replicate number)
(%Effluent) 1 I
2 3
4 Mean Control 0.978 0.991 0.875 0.893 0.934 11.3%
0.832 0.922 1.037 0.871 0.916 22.6%
0.797 0.793 0.882 0.973 0.861 45.2%
0.738 0.910 0.852 0.888 0.847 72.6%
0.859 0.855 0.984 0.950 0.912 100.0%
0.859 0.882 0.927 0.886 0.889 Intake 0.754 0.7?25 0.669 0.757 0.726 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5o: TUc = 100/IC2 5 Page 5 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 11 - 18, 2010 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 1
2134151617
(% Effluent) 134_
Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(%
Effluent)
Data (replicate number) 1 2
3 [ 4 5
6 [7 1 8 1 9.l 10 IMean Control 30 28 30 27 27 31 28 29 30 28 28.8 11.3%
30 34 29 31 33 33 31 30 30 32 31.3 22.6%
33 30 34 34 31 32 34 30 30 36 32.4 45.2%
34 36 32 33 34 33 34 31 35 30 33.2 72.6%
39 31 34 34 33 37 31 36 35 34 34.4 100.0%
36 39 35 39 36 32 34 34 32 37 35.4 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC25 Page 6 of 99
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 11 - 18, 2010 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 1
1 1
1
(% Effluent)2 3
4 5
6 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
% EData (replicate number)
(% Effluent) 1 2
3 1 4 1 5 6 I 7 1 8 1 9 1 10 Mean Control 30
.27 31 32 30 30 28 29 29 29 29.5 Intake 37 33 33 34 34 34 37 33 36 37 34.8 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC2 5 Page 7 of 99
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 11 - 18, 2010 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
1 7 Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(% Effluent)
(replicate number) 1 J 2 3
4 Mean Control 0.822 0.979 0.824 0.962 0.897 11.3%
0.936 0.861 0.848 0.970 0.904 22.6%
0.790 0.786 0.905 0.742 0.806 45.2%
0.880 0.845 0.888 0.909 0.881 72.6%
0.864 0.875 0.870 0.861 0.868 100.0%
0.811 0.874 0.808 0.846 0.835 Intake 0.841 0.765 0.791 0.811 0.802 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
I Species Date I Time I Duration I Toxicant Results (IC2) I Pimephalespromelas May 11 - 18, 2010 1115 Ceriodaphnia dubia May 04 - 11, 2010 0908 KC1 0.72 g/L NaCl 1.09 g/L Page 8 of 99
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 perforned May 11 - 18, 2010.
Test Sample ID Temperature CC)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pnmhos/cnO (mg/L CaCO3) (mg/L CaCO3) Chlorine (ng/L) 24.7 24.5 7.9 7.8 7.53 7.57 329 63 95 C
24.6 -
24.8 24.2 - 24.7 7.7 8.1 7.6 8.1 7.41 - 7.64 7.51 -
7.61 324 -
336 62 64 94 98 11.3%
24.7 24.5 7.8 7.8 7.54 7.56 304 24.7 -
24.9 24.4 - 24.7 7.6 8.1 7.6 -
8.0 7.46 - 7.60 7.49 -
7.63 299 -
314 22.6%
24.8 24.6 7.8 7.8 7.55 7.54 287 24.7 -
24.9 24.3 - 24.8 7.6 8.1 7.6 -
8.0 7.47 - 7.61 7.50 -
7.63 282 -
294 24.8 24.6 7.9 7.8
.7.55 7.54 255 45.2%
S 24.7 -
24.9 24.2 -
24.9 7.7 8.2 7.6 8.1 7.47 - 7.60 7.50 -
7.63 252 258 72.6%
24.8 24.6 7.9 7.8 7.55 7.53 209 24.7 -
24.9 24.3 - 24.7 7.8 8.2 7.7 -
8.1 7.46 - 7.60 7.48 -
7.61 202 -
214 100.0%
24.9 24.5 7.9 7.9 7.54 7.52 167 61 68
< 0.10 24.8 -
25.0 24.3 - 24.7 7.8 8.2 7.6 -
8.1 7.46 - 7.58 7.46 -
7.59 162 -
169 60 62 67 69
<0.10-
<0.10 Intake 24.8 24.5 8.0 7.8 7.54 7.51 166 61 74
< 0.10 24.6-25.0 24.3 - 24.6 7.8 8:3 7.6 -
8.1 7.47 - 7.59 7.45 -
7.58 160 -
171 58 62 73 76
<0.10-
<0.10 Control 24.8 24.8 7.9 7.9 7.53 7.60 329 63 95 24.7 -
24.9 24.6 -
25.1 7.7 8.1 7.6 -
8.1 7.41 - 7.64 7.54 -
7.65 324 -
336 62 64 94 98 11.3%
24.8 25.0 7.8 7.9 7.54 7.60 304 24.7 -
24.9 24.8 -
25.2 7.6 8.1 7.6 -
8.1 7.46 - 7.60 7.54 -
7.66 299 -
314 26%
24.8 25.0 7.8 7.9 7.55 7.59 287 22.6%
24.7 -
24.9 24.7 -
25.3 7.6 8.1 7.6 -
8.1 7.47 - 7.61 7.55 -
7.64 282 -
294 "2%
24.8 25.0 7.9 7.9 7.55 7.59 255 4
24.7 -
25.0 24.8 -
25.3 7.7 8.2 7.7 8.2 7.47 - 7.60 7.54 -
7.64 252 -
258 24.9 25.0 7.9 7.9 7.55 7.58 209.
72.6%
24.8 -
25.0 24.9 - 25.1 7.8 -
8.2 7.8 8.2 7.46 - 7.60 7.52 -
7.63 202 -
214 100.0%
24.9 24.9.
7.9 7.9 7.54 7.57 167 61 68
< 0.10 24.8 -
25.1 24.7 - 25.1 7.8 8.2 7.8 -
8.2 7.46 - 7.58 7.51 -
7.61 162 -
169 60 62 67 69
<0.10-
<0.10 Intake 24.9 25.0 8.0 7.9 7.54 7.55 166 61 74
< 0.10 24.8-25.1 24.8 - 25.3 7.8 8.3 7.7 -
8.2 7.47 - 7.59 7.46 -
7.60 160 -
171 58 62 73 76
<0.10-
<0.10 Overall temperature CC)
Pimephales promelas Ceriodaphnia dubia Average Mininmu 24.7 24.2 24.9 24.6 Maxinumn 25.0 25.3 Page 9 of 99
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas Tests, UV-treated Sequoyah Nuclear Plant (SQN) Outfal1101 performed May 11 - 18, 2010.
Test Sample ID Temperature (°C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Initial Final Initial Final Initial Final (prmhos/cm) (mg/L CaCO3) (mg/L CaCO3)
Control 24.8 24.4 7.9 7.8 7.58 7.54 315 62 91 24.7 -
24.9 24.2 -
24.7 7.8 -8.1 7.6 8.0 7.52 -
7.63 7.48 -
7.60 311 320 60 64 88 94 11.3%
24.9 24.7 8.0 7.8 7.60 7.53 304 24.8-24.9 24.5 -
24.8 7.8 -
8.2 7.6 7.9 7.52 -
7.64 7.44 -
7.59 300 -
309 2.%
24.9 24.6 8.0 7.8 7.59 7.53 285 Eý.
22.6%
24.8 -
25.0 24.4 -
24.8 7.9 8.2 7.7 8.0 7.52 -
7;64 7.44 -
7.60 274 -
296 45.2%
24.9 24.6 8.0 7.8 7.60 7.53 254 24.9 -
25.0 24.4 -
24.8 7.9 -
8.3 7.6 -
8.0 7.53 -
7.65 7.44 -
7.59 247 -
259 24.9 24.6 8.1 7.8 7.59 7.52 212 72.6%
24.9 -
25.0 24.5 -
24.7 7.9 8 8.3 7.6 7 8.0 7.51 -
7.63 7.44 -
7.58 208 -
219 100.0%
25.0 24.6 8.1 7.8 7.58 7.50 170 61 70 24.9 -
25.2 24.5 -
24.7 7.9 8.3 7.6 8.1 7.50 -
7.61 7.42 -
7.56 165 -
173 60 62 69 71 Intake 25.0 24.6 8.1 7.8 7.57 7.52 165 61 68 24.8 -
25.2 24.3 -
24.8 7.8 8.3 7.6 8.1 7.50 -
7.62 7.44 -
7.56 159 -
167 59 62 67 69 Overall temperature (0C)
Pimephales promelas Average 24.8 Mininumn Maximum 24.2 25.2 Page 10 of 99
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected May 09 - 14, 2010, showed no toxic effects to fathead.
minnows or daphnids.
The resulting IC 25 values, for both species, were > 100 percent.
Exposure of daphnids to intake samples resulted in no significant difference from the control during this study period.
Minnow growth in the intake samples was significantly lower than the control.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
Page 11 of 99
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None Page 12 of 99
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by SM 2550 B.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM 2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KC1 or NaCl.
Sodium Chloride (NaCI crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version.5.0 was used for statistical analyses.
Page 13 of 99
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 2 1st Edition, 2005.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 99
Sequoyah Nuclear Plant Biomonitoring May 11.-
18, 2010 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010 Date Sdiuli Towerbrom P6E41(12)??
PCL-401 CL3 Cuprostat-I H,p1-yohioite mg/L nm/Ti>
mg/L m
PF 1)"
d L
TRC Phospf*ihfe Copolymer Dh/I f mg/L
(
/
t
- J Azole 03/12/1998 0.16 03/13/1998 0.015) 03/14/1998 0013 03/15/1998 0.030 03/16/1998 O.01:
03/17/1998 0.02(
03/18/1998 0.018 1
09/08/1998 0,
FlIff I
().(1 0.005 09/09/1998 0.003) 0")31 0.011 09/10/1998
(),)14 1 6.()
0.021 09/11/1998 130055 0.019 09/12/1998
()0.O0
.044>1 0.015 09/13/1998 I0.00 0.041 0.015 09/14/1998 A0008 0.044 1
0.015 02/22/1999 0A001 02/23/1999 0,005 02/24/1999
()009 02/25/1999 02/26/1999 02/27/1999 02/28/1999 0.00t 08/18/1999 0.015
- 0.
0.024 0.H00 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.07O 0.024 0.120 08/21/1999 0.022 1 20408 0.024 08/22/1999 0.022 0)068 0.024 08/23/1999 0.025 0.068 0.024 0I0N 08/24/1999 1
0.016 1
t0 67?
0.023 1 0.020 Page 16 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 10i) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010
'Date Sodiffiii Towerbrom PCL-22 PCL-401 (1463 Cuprostat-1-Ph130M Hypochiorite' mg/L mg/L 7 mg/L ingL PF ImIg/L 1 mg,/L TRC Phosphate Copolymer
'DMAD ng/L Quat TR..
Azole
)1/31/2000 @
< 0.002 O'I0'2)6 0.009
)2/01/2000 c 0.011
()0 02*6 0.028
)2/02/2000 0.028 0.026 0.009 0.*0*0 o2/03/2000 0.00 0.027 0.009
)2/04/2000 0.006
- 0. 02 1, 0.009
- 0,05 0.109
)2/05/2000
< 0.002 0.027 0.009
)2/06/2000
< 0.002 1
0027 0.009
)7/26/2000
<0.0057 0.055 0.019
)7/27/2000 0.019 0.055 0.019 17/28/2000 0.0088 j0.053.
0.018 0.004 0.108 17/29/2000
< 0.0088,
+0,055 0.019 7/301/2000 @<
0.0076
- 0. 0 055 0.019 7/31/2000
< 0.0152 0.055 0.019
- 0006,
-6 18/101/2000
< 0.0141 0.305' 0.019 0.005 1j 2/11/2000,
0.0143 U.025 0.020 (j.00" 2/12/2000 0.0092 0
.025 0.020 005 2/13/2000
<0.0120 07 0
0.020 2/14/2000
<.0.0087
.0.0270 0.020 2/15/2000 0.0120 6 0025 0.020 0.
2/16/2000
<0.0036
). 0.25 0.020 2/17/2000
< 0.0036 0,025 0.020 18/26/2001 0.017
- 0. 0 6 0.021
~0,06 18/27/2001
<0.0096 o0.
H o 0.021 0,00,)
002 18/28/2001
<0.0085 0.0 0.021 5
18/29/2001
- ,<0.0094 1
7 O'O.O 0.020 0.005 0,021 8/30/2001
<0.0123
.00689 0.021 0.U005 8/31/20011
<0.005
'12O0.05!9 0.020 1
1/25/2001
<0.0044 1/26/2001
<0.01319 0024 0.02 00Q5 1/27/2001 0.0137 0023 0.019 0.007 1/28/200 1
<0.0089
().H22 0.0 19 0.006 1/29/2001 0.0132 0.024 0.02 0.007 1/30/2001 1 < 0.0043
- 0. H'0.624 0.02 2/09/2001
<0.0042 i"K i
2/10/2001
+ -
<0.0042 2/11/2001
<0.0104
+
2/12/2001 0.0128 0.021.
0.02 0.008 2/13/2001
<0.0088 0.024 0.02 2/14/2001 1 0.0134 1
0.024 0.02 0.007 Page 17 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010 Date Sbdii Towerbrom PCL-222 PCL-401
-IL 363 Cuprostat-FH-130N I k pOcurite mg/L mg/L*
mg/L m,/L PF ig/L m/
TRC Phosphate Copolymer IMAD mg/L 0ua1
>~,.
Azole
)1/02/2002
<0.0079 00,3 0.02
'0000
)1/03/2002
< 0.0042
(<).........
0.014
)1/04/2002 0.0124 h'(',>*0241 0.014 O009)
)1/05/2002
< 0.0042
[
)1/06/2002
< 0.0042
)1/07/2002 1 < 0.0089 0,-02) 0.014 0006
)2/24/20021
< 0.004 1
)2/25/2002
<'0.004
'0023*
ý 0.023
)2/26/2002 0.0143 0*6 'O2) 0.023 I0007
)2/27/2002
< 0.0041
(..02)3 0.023
)2/28/2002
< 0.0041 0,
0)-024 0.008
)3/01/2002 1* < 0.0041 0.02.4 0.008
)5/05/2002
)5/06/2002 058*,
0.02 1 1 15/07/2002,
- 0.085 0.02 15 15/08/2002 0.056 0.019 15/09/2002 0.057 0.02
- 0. 00141 15/10/20021 1
0.056 q 0.019 __
18/04/2002
<0.00588 18/05/2002
<0.0058 (0053 0.018 18/06/2002 0.0092
().05*3 0.0,18 18/07/2002
<0.0 107
- 0. 055 0.0 19 0 007i 18/08/2002
<0.0061 055 0.019 18/09/2002 0.0152 1
0.054 1 0.018 0008 0/06/2002
<0.00497 0.1 0/07/2002 0.0153
- 5'41y 0.018 0009 0/08/2002
<0.0092
).05'4 0.018 0-007 0/09/2002 0.0124
().05 J3)K 0.018 0009)
W 0/10/2002 0.0134 0).05"4
~0.018 0009(y 0/11/20021
~
<0.0042 1
0.()5,1 0.018 1
11/12/2003
<0.0035
-1/13/2003
<0.006 0.019 0
11/14/2003
<0.01 18 0.026 0.020 11/1 5/2003
-<0.0063 0.026 0.020 00 11/16/2003
<0.0034 0.02'6 0.020 11/17/2003
<0.0034 0.026 0.009--
4/06/2003
<0.000
.07 4/07/2003
<0.0189 0.021 14/08/2003
<0.0117 0.021 14/09/2003
<0.0139 i
,1 0.021 0*0 16 14/10/2003
<0.0113 0.021 0018 14/11/20031
<0.0073 1
0.022 Page18 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010 Date S'duml Towerbrom P1@L,222 2 PCL-401 (LA-303 Cuprostat-I'C HIý pmc4h1o)riit mg/L mIII'L mg/L mg/Ld PF mgIII ifgI 1
l' TRC Phosphate Copolymer [DMAL zmg1i
'15/2003
< 0.0045
'16/2003
< 0.0037 0
- 5 Y
0.020 0J-
/17/2003
< 0.0048 0)041 0.014 W
'18/2003
< 0.0048 0.04 0.014 02
'19/2003
< 0.0085 0.05X 0.020
'20/2003
< 0.0048 0, 0 0.020
()2
'03/2003
<0.0050
'04/2003
<0.0050 0.058 0.020 I
'05/2003 *
<0.0051 0,057 0.020 (Y)
'06/2003
<0.0084 (0,7W 0.020
-0
'07/2003 0.0129 0.057 0.020 0
'08/2003.
0.0153 0.037 0.020 0.009
'05/2003
<0.0043 0.05i 0.0 0
/06/2003
<0.0043 0.057 0.020 0.02
/07/2003
<0.0090 0.057 0.020 0.02!
/08/2003.
<0.0106 0.057 0.020 0.02
/09/2003 0.0181 0.026 0.022
/10/2003 1 0.0183 0O.026 0.024 J.00-
/01/2004 0.0093 007' 0.009 02/2004
<0.0034 0.026 0.009 03/2004
<0.0034
(.02G 0.009 04/2004 0.0124 "fO2 0.009 O....
05/2004
<0.0034 0 026 0.009 06/2004 0.0105 0.026 0.009 1
0-4/2004
<0.0123 0.,026 0.019 7-12 05/2004
<.144 H0.026 0.014
()0.009 002
<0.0146 0.037 0.013 07/2004 0.0227 0.058 0.020 (0).(090 i
08/2004 0.016 0.021 09/2004
<0.0104 0.058 0.020
-9 04/2004 0.0217 0.0571 0.019 05/2004
<0.0085
.oA57 0.020 009 06/2004
<0.0077 0,
b.058 0.020 f.3J 07/2004 0.0252 0.056' 0.0 19 0.03-08/2004 0.0223
() 0.57 0.019 0.009 09/2004 0.0182 0.057 0.020 0.009 Page 19 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010 Soditmi Towerbrom PF.....
PCL-401 UL-363 Cuprostat-PF 1-130M Nalco Hvpd dhrl mg/L m
mg/L ing/L mg/L mg/1-73551 e
TRC n'osnphaw Copolymer DMAD Azole Quat mg/L EG/PO 4
<0.0187 0.000 0.014
<0.0192 0.047 0.030 I
<0.0233 0.048 0.016 4
<0.0149 0.047 0.016 041 I *
<0.0149
() I 4
I 0.017 4
<0.0253
.048 0.017 IOý02
<0.0042 I0010
.LO
<0.0116 J0,8 0.010 0.007
~ ~
<0.0080 Th28 0.010 0.0199 0.028 0.010-
<0.0042 02 81 0.010 0.0155
- 0. 2 8 0.010 0.007 0.0063 0.0043 0.0103 0.0295 0.0129
~~
.0.0184 0.0109 0o M 0.009
/
0.0150 40.02G 0.009 0.0163 0.026 0.009 0.0209 0.026 0.009 0.014 0.0242 0.020 0.009 0.0238 0, 0054 0.018 0.014 0.0068 0.0112 0.0104 0.0104 0.0117 0.0165 0.0256 0.0234 0.0231 O
1 0.0200 0.0116 Page 20 of 99
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 14, 2010 Date odi.i..
Towerbro P.
22:2 PCL-401 C' L-36r3 5 Cuprostat-PFH IM Nalco 1H1-.
yvpodlorite mg/L mgiL*'.
mg/L m-1 mg/L n/g/L 73551 m
11/L TRC Phosphate Copolymer DMA\\D Azole i
mg/L I Nl EO/PO /
11/12/2006 005 11/13/2006 0.0068 11/14/2006 0.0143 11/15/2006 0.0068 11/16/2006 0.0267 11/17/2006 0.0222 11/26/2006 V0.0188 11/27/2006 0.0138 11/28/2006 0.0120-11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 05/29/07 05/30/07 0.0084 0.017 05/31/07 0.0103 06/01/07 0.0164 0.017 06/02/07 0.0305 12/02/07 0.0241 12/03/07 0.0128 12/04/07 0.0238 12/05/07 0.0158 12/06/07
,,, 0.0162 12/07/07 0.0175 04/13/08 0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.014 10/26/08 0.0260 10/27/08 0.0151 0.017 10/28/08 0.0172
,,0.
10/29/08 0.0154 0
)
10/30/08 10/31/08 0.0086 Page 21 of 99
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test
- Sampling, March 12, 1998 -May 14, 2010 Date Sodium Towerbrom P
'L PCL-401 CLS3.
Cuprostat H 130MF Nalco SpectII I
-150MI I 1\\ypoc ritel mg/L 222 mg/L mg
-PFmg/L mgL 73551 I 1300 mg f,
>mll",
TRC Copolymer IP Azole Q
1 l
I PC Hnhoshn EG/PO Quat1 02/09/09 0.0237 0.017 02/10/09 0.0104 0.021 02/11/09 0.0155 02/12/09 0.0106 0.017 02/13/09 05/10/09 0.0129 05/11/09 0.0415
-1/2 04 05/12/09 0.0053
-)'03 96 05/13/09 0.0049 003. 6 05/14/09
<0.0141 9
3974k 051/9<0.0160-11/16/09 V
0.0152 11/17/09 0.0255 11/18/09 0.0306 11/19/09 0.0204 11/20/09
.0.0093 0 5/09ý/10O 0.0192 05/10/10 0.00055 055'7,
.0039 05/13/10 0.0041 05/14/10 0.00990.39 MSW 101 mg/L Phosphate Page 22 of 99
Sequoyah Nuclear Plant Biomonitoring May 11 - 18, 2010 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 99
BIOMONITORING CHAIN OF CUSTODY RECORD Page
_1_ of -1 Y
'I
(~ent: TVA Pfect Name: Sequoyah NP Toxicity 1*. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Adam Deimling & John F. Lane Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Client 1
p ecify): Express Courier General Comments:
Adam Deimling John F. Lane Samples remained on ice throughout sampling and transport to lab. Dissolved Metals sample filtered and place on ice.
Fax:
828-350-9368 Y -
Y a -,
Y -
Y Field Identification /
Sample Description Grab/Comp Collection Date/Time Container Number &
Volume 0~
,.-11 Flow (MGD)
Rain Event? I1 12
~~l~~Use (Mark as Appropriate)
T(
I Sample Custody - Fill In From Top Down
-4~
LOSOT SeOO& w..ipfOv C-vaf fM't tev Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time AtU.
6AL y Adam Demln
-574,c
ýl (L-7Ie 7' Express Courier y3At4
~-
~
/2/5~~
Express Courier 4'
I E ETS
-S-IO-,
i.4S Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1-of U 4ent: TVA Pieject Name: Sequoyah NP Toxicity Pz.
Number: N/A Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 FedEx UPS Bus Client Delivered By (Circle One):
Other (specify): Express Courier Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Adam Deimling & John F. Lane Fax:
828-350-9368 Samples remainedAice throughout sampling an'iw lab. Dissolved Metals sample filtered and place on ice.
Field Identification/
Grab/Comp Collection Date/Time Container Flow Samle esciptonNumber (MGD)
Rain Event?
Lbrtr s
Volume (Mark as Appropriate)
C ollected
.iii:!
ii:
ii il iiiiii:ii! ii!::ii:l:*!'
Date Time Yes If Yes, No Trace
.TS Log' Arrival"Temp'*-By Time Appear-(mm/dd/yy)
(EST)
Inches
'Number (I) ance 05/11/110-0656-1 SQN-101-TOXA Comp
- 05/12/10 0556 1 (2.5gal) 1639.25 X
100S\\-L.6 SQN-101-TOX B Comp 05/11/10 056 S
TTo0 1020012 a
NX_:
______1 05/12/10 1 0556 1(2.5gal) 132 X
1
'(*-,,
SQNIN-TO Cmp 05/11/10-0713-05/12/10 0613 125gl N
A.9 Sample Custody - Fill In From Top Down 4
C.orovA seAjs st*-rec.
s,%W s Aec.et\\M trA 6oob LtDAIO -
)O A&Se.ar l Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time AUL SIY4tfiS Adam Deimling
-57.,/--
a ?'&
"Express Courier
- 6 c
'-0 Express Courier O
m MILS lO E
ETS 1t2.-10 1 L-. S rV 4
1 1
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive.the samples with ample time to initiate testing within that time frame. Samples shipped
.overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page
_1 of -I C *ent:
TVA P 'ect Name: Sequoyah NP Toxicity P)@. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Adam Deimling & John F. Lane Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Client Other (specify): Express Courier General Comments:
- f Adam Deimling I.
I John F. Lane Fax:
828-350-9368 Samples remainede*e throughout sampling and transport to lab. Dissolved Metals sample filtered and place on ice.
Field Identification /
Grab/Comp Collection Date/Time Container Flow
- !(*iCJ.
Sample Description Number &
(MGD)
Rain Event?
Laboratory Use.....
Volume (Mark as Appropriate)
C ollected..........
eieYes If Yes, No Trc T
~
ArvlTemnp, y
Tm Appear-(mm/dd/yy)
(EST)
Inches Number ET c*c*
SQN-101-TOX_____
A
__Comp_
05/13/00/4/10 065-553 1 (2.5gal, 1849.92 X
2!.::
- 7.
i~i~** ii::::ii:ii ii{
- ~!i:!£***i!:!;;***
SQN-101-TOX A Comp 05/13/10-0653-1 (2.5gal) 1649.92 X
'j!bq:"C4 2:yziri*s SQN-101-TOX B Comp 05/13/10-0653-1 (2.5_gal)
NA X
(.q'C s-05/13/10-07113
'11%
SQN-INT-TOX Cop 05/14/10-0611-1(2.5 gal)
NA X
-A-I$I.O Sample Custody.- Fill In From Top Down
"-* mA ¶UAL&
s
- K-T'.
Shtkres, C...
.Lb ' D Relinquished By (Signature):
Date/Time Received By (Signature):
iADD C..
V'1 to, A
.DateTiTrne ALL 5, Adam Deimling 9/340 1
i7 Express Courier X"
1s
,ej..
5.- /
- /
Express Courier NIIO*
'!O i
,'41 ETS S.+O-I0 ItqzS Sr Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
0 n~omnaTtn ouinIc Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas I
Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0020168 Project #:
t I SS County: Rhea Outfall: 101 II i
I Dilution preparation information:
Comments:
Dilution prep (%)
11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
A-11 &ot.s oa.k Randomizing template:
uo,%k)
Date and times organisms oT-10-10 IoO Incubator number and were born between:
I shelf location:
'3 (
Organism source:
A
.x 6-i Pe os-Oa Artemia CHM number:
C( t*sik Drying information for weight determination:
Transfer bowl information:
pH = ".
S.U.
Date / Time in oven:
os-la&,k)
Temperature = 25.AC Initial oven temperature:
'c.
Average transfer volume:
o, X-514 Date / Time out of oven: o-*.ltdiIO Final oven temperature:
C.
Total drying time:
"iA.
ObJA I
Itso I
ASYO IS Daily feeding and renewal information:
-I Day Date Morning feeding Afternoon feeding.
Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0
to 400W-A IooS'O.O \\
IOOSIO.0o".
Os'-.Oi I OS'r-1-eO 01Uc A
1-,
I
.0 AO0SOSI-.O\\
IOOSO I_11 0$-10"10 3
I oas-
,A I
0S30
,0ool',
IO5" IOOSit. 10 O12S'1-O 45 s I -0 I 0, j -
A b o AM 61 0 1 i
0 s q -t o -13 1 6
0,-11--0
-A iObli
- Oo*lq.OS 1 oslq, to os-q -Ito -tJ1
-6%-0 I
U U
Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
- 07.
< 20%
7-day LC50
) IOoT.
Average weight per initial larvae:
&1 -3 NOEC I1,.
Average weight per surviving larvae:
o.41' &__
0.25mg/larvae LOEC
>iOO7.
ChV
'J I my..
1C25 10 0-.
Page 27 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
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SEnvironmental Testing Solut oInc.
Page 2 of 6 Species: Pimephales promelas Client: TVA / Seauoyah Nuclear Plant, Outfall 101, Non-treated Date:
Survival and Growth Data in-si-IO Day CONTROL 11.3%
22.6%
Non-treated A
B C
D E
F G
H I
J
.K L
0 1t1 10 1
()
I
/0 to 0 t0
/0
/0 t0 1
t 0
1(3 10 /3
/0 13 O
1(3 /
0 O
2 10 C)
/0
/
0 10 to t3 O
0 /0
/1 3__
,O 1
/U 10
/0
/0 to0
/0
/0 10
/0 40 (0
10 10 to 10 1
/
/0
/0 to0 1
!0 10 I0 (0
D 10 10 10 (0
D to (0 0
6 I
(
,0
/
I 3
1 /0 to
/0 t0 to
/0 lb.16 1I
/0
./
o te
/0 01 A = Pan weight (mg)
Tray color code:: N th
'07 Analyst:
I 1','96 A l
1,,1 13-13 13,qq iq'11 j* I3,qAo fLi.1 )4.(o q,o Date:
o,',-
B = Pan + Larvae weight (mg)
Analyst: LRI 2A,13 Z.'lp 2-0.711 2.tI-22..6 213.!(p Z.5q 22..tv Z5I.13 22.10 2Z.83 Z3.75 Date:
0" 2 2 1 0 C = Larvae weight (mg)
= B - A q.91
&.I S R. b a bM - q.12 -
10
,1 1 V I I"'M
,0
- k. U. q -1 3 Weight per initial number of larvae (mg)
= C / Initial number of larvae 0
0.
A" PV 1"
A~.
0.'
A.
0#
Average Percent weight per reduction Initial from
-lt number of control (%)
larvae (mg) o.JI1,
2 0*l.
T. \\
n, 7 I
I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
IComments:]
Page 28 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I.
Page 3 of 6 Environmental Testing Solutions& Inc.
Species: Pimenhales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date:
0S. i - 0 I
I I
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I Survival and Growth Data Day 45.2%
72.6%
100%
M N
0 P
Q R
S T
U V
W X
0
/1 10
/b /0 10 10 1O 10
/0 10
/0
/0 1
1______
10 10 10 10 101 /0 ID 10 10
- 10) 10 I/0 2o I0
.10 1 /0 10 /
1 to 1to 0 io/0 3/
10
,10 I0 to
/0 10
/0 to
/1
/Q0
/0 5
/0
/0 (0
(0 10 101t/
/0 (0
/0
'0 1O 7
-Fism/0
/0
/0 1
0
/0 C)
D0 to
( 0
/0 (O
t o
A -Pan weight (mig)
Tray color code:: ft Analyst: b1
.. !Yh 13-5ý 14-03 13-Sq 13,11
%~iN53.531 N.-34L Klq 13,59 ItA.('N-Date: _bS,_____
B -Pan + Larvae weight 2.4* 3-01
.~
(ing) 1.0.75' Z+I19? 1755 22.'47 2230S Z-3.Ao7 )2!9 1 luriA
),' 12 0 23-30 Date:
IS.tLtt10 C Larvae weight (mg)
=BAnaytTry&.e5e:
'2L' *31i.V Iq
.&3I&S 18,11
&S,'7 9&,&i *,'S1 I&.60
,q a It.,
Is.*
Weight per initial number of(larvae(mg)
V4 C / Initial number of larvae Date:-
Average Percent weight per reduction initial from control 0.*A1
- I,*7@
O.l-1o
-.A 0.
number of
(%)
larvae (mra)
Comment codes: c = clear, d = dead, fg = fungus, k - killed, m = missing, sk - sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
I I
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Comments:
Page 29 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
"D SEnvironmental Testing S;olutions, Inc.
Species: Pimephales vromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Page 4 of 6 Date:
OS*ik-10 I
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I Day 100% Intake Y
Z AA BB 0
10 1o
/,
10 I' I
to It 10 10 1
1 IQ 0 3 ~
I 10 10 1/U 4
5 10 I(0 to to 6
/0 lo 10
/0 7
I A = Pan weight (mg)
Tray color co~e::
!Analyst: b-\\2ý
ý 1-3 L 1q.I1-Date:
< *'*,2 B = Pan + Larvae weight (tug)..
2 b.q l1 Analyst:
LAO.
Date: OS-1.2.- O C = Larvae weight (mg)
=B-A L,. (I1 Weight per initial number of larvae (mg)
= C / Initial number of larvae Average Percent weight per
[reduction initial from control 12.L.
- 2. b.-
numuber of I(%/)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, ig - unusually large, d&r = decanted and returned, w = wounded.
I Calculations and data reviewed:
I Comments:I I
Page 30 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
.1%....
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 11 - 18,2010 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number.
6155 Revehwedhby:
f' Not fir-Cumplie.
=,e, t o**
c""1...
~~Not fir Compliancc Ass~essmflent, It,.~a Laboratory QC C-cemtral*o (%)
Reimi hante onmber-Fldut mbereufvu A=-Pelawt t(ml;)
B=Pe+ L a
Leeewdahn)
Weightwe8rhlt/
dng M"anwsdot/i C-01,ktee af,..vlateon Weighltlantt mber Mm ervivel 1,
.Mwdghtljlblted Coetkekatof P-eet redectmo. fin I
e reht(rna)
-A-B nnmberolatvwe(ms)
Survivit.mn..erd Om 6P"*S oflarvereg)
(%)
a.nber.-l-rva vtaelao. 04-*r.
-etrd (%)
A to0 10 14.45 24.23 9.78 0.978 0.978 C
0.934 6.3 0991 100.0 0.934 6.3 Not applicable Cn 10 10 11.96 20.71 8.75 0.875 0.875 D
10 R
12.71 21.64 8.93 0.893 0.893 E
10 10 13.73 22.05 8.32 0.832 0.832 F
10 10 13.94 23.16 9.22 0.922 0.922 G
10 10 14.17 24.54 10.37 1.037 1.037 100.0 0.916 9.7 2.0 H
10 10 14.25 22.96 8,71 0.871 0.871 1
10 10 13.96
" 21.93 7.97 0.797 0.797 226J 10 10 14.17 22.10 7.93 0.793 0.867.909310.0869978 22.6%
K 10 10 14.01 22.83 8.82 0.882 0.861 9.9 0.782 L
08 10 14.02 23.75 973 0.973 0.973 M
10 10 13.37 20.75 7.38 0.738 0.738 45.2%
N 10 10 15.69 24.79 9.10 0.910
. 0.847 9.0 0.910 9.0 9.3 0
10 10 14.03 22.55 8.52 0.852 0.852 100.0 0.847 P
10 10 13.59 22.47 8.88 0.888 0888 Q
10 10 13.71 22.30 8.59 0.859 0.859 72.6%
R 10 10 15.07 23.62 8.55 0.855 0.912 7.]
0.855 S
10 10 14.58 24.42 9.84 0.984 0.984 T
10 10 13.57 23.07 9.50 0.950 0.950 U
10 10 1436 22.95 8.59 0.859 0.859 100%
V 10 10 14.19 23.01 8.82 0.882 0889 3.2 8
0.882 W
10 10 13.59 22.86 9.27 0.927 0.927 X
10 10 14.44 23.30 8.86 0.886 0.886 Y
to 10 14.82 22.36 7.54 0.754 0.754 to-0013.16 "20.41 7.25 0.725 0.730.725 INN. Intake 0.6
- 10.
0760.
2 AA 110 14.17 20.86 6.69 0.669 0.726 5.6 BB 10 10 16.16 23.73 7.57 0.757 0.757 Outfall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
0.1195 12.8 0.0695 7.4 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound detenmined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 mid EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OIL
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 11 - 18,2010 Statistical Analyses 1.0 Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/11/2010 Test 11):
PpFRCR Sample ID:
TVA / SQN101 End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Cone-%
1 2
3 4
Control 0.9780 0.9910 0.8750 0.8930 11.3 0.8320 0.9220 1.0370 0.8710 22.6 0.7970 0.7930 0.8820 0.9730 45.2 0.7380 0.9100 0.8520 0.8880 72.6 0.8590 0.8550 0.9840 0.9500 100 0.8590 0.8820 0.9270 0.8860 Intake 0.7540 0.7250 0.6690 0.7570 Transform: Untransformed 1-Tailed Isotonic Conc-%/,
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean Control 0.9343 1.0000 0.9343 0.8750 0.9910 6.286 4
0.9343 1.0000 11.3 0.9155 0.9799 0.9155 0.8320 1.0370 9.720 4
0.378 2.410 0.1195 0.9155 0.9799 22.6 0.8613 0.9219 0.8613 0.7930 0.9730 9.876 4
1.473 2.410 0.1195 0.8772 0.9389 45.2 0.8470 0.9066 0.8470 0.7380 0.9100 9.032 4
1.760 2.410 0.1195 0.8772 0.9389 72.6 0.9120 0.9762 0.9120 0.8550 0.9840 7.130 4
0.449 2.410 0.1195 0.8772 0.9389 100 0.8885 0.9510 0.8885 0.8590 0.9270 3.184 4
0.923 2.410 0.1195 0.8772 0.9389 Intake 0.7263 0.7774 0.7263 0.6690 0.7570 5.618
.4, Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96793216 0.884 0.215280069
-0.7446185 Bartlettes Test indicates equal variances (p = 0.64).
3.40016365 15.0862722 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
0.11945887 0.12786607 0.00457047 0.00491397 0.48466599 5,18 Treatments vs Control Linear Interpolation (200 Resamples)
Point SD 95% CL(Exp)
Skew IC05 19.547 IC>1 IC15 IC20 IC25 IC40 IC50
>100
>100
>100
>100
>100
>100 sqnlOl_O5111Odata
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated "0
o SEnvironmental Testing Solutionis, Inc.
May 11 - 18, 2010 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/11/2010 Test ID:
PpFRCR Sample ID:
TVA / SQN101, Intake End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Non-treated Conc-%
1 2
3 4
Control 0.9780 0.9910 0.8750 0.8930.
11.3 0.8320 0.9220 1.0370 6.8710 22.6 0.7970 0.7930 0.8820 9.9730 45.2 0.7380 0.9100 0.8520 p.8880 72.6 0.8590 0.8550 0.9840 0.9500 100 0.8590 0.8820 0.9270 0.8860 Intake 0.7540 0.7250 0.6690 0.7570 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Control 0.9343 1.0000 0.9343 0.8750 0.9910 6.286 4
11.3 0.9155 0.9799 0.9155 0.8320 1.0370 9.720 4
22.6 0.8613 0.9219 0.8613 0.7930 0.9730 9.876 4
45.2 0.8470 0.9066 0.8470 0.7380 0.9100 9.032 4
72.6 0.9120 0.9762 0.9120 0.8550 0.9840 7.130 4
100 0.8885 0.9510 0.8885 0.8590 0.9270 3.184 4
- Intake 0.7263 0.7774 0.7263 0.6690 0.7570 5.618 4
5.817 1.943 0.0695 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.88218808 0.749
-0.2763905
-1.9388544 F-Test indicates equal variances (p = 0.57) 2.0715251 47.4672279 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.06947945 0.07436923 0.086528 0.00255692 0.00113375 1, 6 Treatments vs Control sqnlOl_OSlllOdata
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 11 - 18,2010 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 n
nDaily Chemical Analyses Environmental Testing solutions, Inc.
Project number:
6155 Reviewed by:
Concentration Parameter Da 0 Da 1
Da 2 Da 3 Da 4 Da 5
Da6 Initial Final Initial IFinal IInitial IFinal IInitial Final Initial Final Initial IFinal IInitial Final PH(SU) 7.58 7.58 7.461 7.531 7.431 7.571 7.41 7.56 7.61 7.60 7.591
.7.511 7..64 7.61 DO - gL) 7.8 7.8 7.81 7.81 7.9 7.61 7.9 7.7 7.7 8.0 8.11 8.11
- 7.
7.9 Conductivity (puhos/cm) 326 3324 332 Control Alkalinity (mg/fL CaCO3) 64 64 Hardness (mg/L CaCO3) 94 Temperature (C9 24.8 24.6 24.6 24.5 24.7 24.4 24.7 24.7 24.7 24.5 24.7 24.2 24.8 24.6 PH (SI])
7.54 7.57 7.591 7.491 7.54 7.57 7.46 7.54 75 7.9 7.60 7.51 7.50 7.63 1.% DO (mg/L) 7.8 7.8 8.01 7.8 7.9 7.6 7.8 7.6 7.6 808.1 8.0 7.6 7.6 11.3%
Conductivity (hmboslcm) 301 302 299 299 308 308 314 Temperature 0C) 24.7 24.6 24.7 24.5 24.7 24.7 247 244 24.7 24.4 24.7 24.7 24.9 24.4 pH (SU])
7.56 7.54 7.60 7.50 7.55 7.53 7.47 7.55 7.56 7.56 7.61 7.50 7.51 7.63 DO (nigL) 7.8 7.8 8.0 7.8 7.91 7.6 7.81 7
7.71
- 7.
9 8.1 8.0 7.6 7.6 22.6%
Conductivity (pmhos/cm) 284 285 294 282 290 292 94 Temperature CC) 24.8 24.5 24.7 24.3 24.8 24.7 248 248 24.8 24.5 24.8 24.7 24.9 24.4 PH SU) 7.56 7.53 7.601 7.50 7.57 7.50 747 7.56 7.56 7.56 7.59 70 7.52 7.63 45.2%
78 78 80 78 80 77 78 7.6 7.8 782 7.7 7.7 42%
Conductivity (jthos/em) 225 252 256 257 258 Temnperature CC) 248 245 247 243 249 249 248 248 248 2
248 24 24.9 24.5 pH(SU) 7.56 7.51 7.58 7.491 7.55 7.49 7.46 7.54 7.56 756 7.60 748 7.51 7.61 DO gL) 7.8 7.8 8.0 781 80 7.7 7.8 7.7 7.8 81,2 8.1 7.8 7.7 7.% Conductivity (tuhos/cn) 210 213 206 202 20*6 212z 214
______Temperature OC) 24.9 24.7 24.7 24.6 24.9 24.6 24.8 24.7 24.8 24.3 24.81 24.61 24.9 24.5 PH (SU])
7.55 7.53 7.58 7.46 7.54 7.49 7.46 7.52 7.551 7.561 7.571 7.461 7.52 7.59 DO (mg/L) 7.81 7.81 8.0 7.8 8.0 7.8 7.8 7.6 7.81 8.11 8.21 8.11 7.9 7.8 Conductivity (pmhos/cm) 100%
Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3 )
Total Residual Chlorine (mg/L)
<0.10
_Temperature CC) 25.0 24.4 24.8 24.7 25.0 24.6 24.8 24.5 24.8 24.3 24.8 24.5 25.0 24.6 PH_____(SU)_____
7.551 7.531 7.591 7.45 7.511 7.491 7.471 7.53 7.57 7.53 7.57 7.48 7.552 7.58 D m/)7.81 7.81 8.01 7.8 8.11 7.81 7.91 7.6 7.9 8.0 8.3 8.1 8.0 7.8 C o n d u t iv i y
1ho s / m 100% Intake Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
Total Residual Chlorine (mg./L)
<0
<0.
_______Temperature (C) 1 25.01 24.41 25.01 241.5 24.91 24.51 24.71 24.6 24.71 24.31 24.61 24.51 25.0 24.6
a i
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Environmental Testing Solutions, Inc.
Species: Pimephales promelas Client: TVA / Seauoyah Nuclear Plant, Outfall 101, Non-treated Daily Chemistry:
Page 5 of 6 Date:
OS-j!- iO Day Concentration Parameter Conductivity CONTROL (pumhos/cm)
Non-treated Alkalinity (ing CaCO 3IL)
Hardness (mpg CaCt 3u/
_______Temperature (0c) 1..
to~.tL'~5
's pH (S.U.)
-7.
1 T 7S-7 1 -S7 i
5 H -7.'1 Y4
-7.5' Ll l
DO (mr/L)
- "7. -5 I
11:3%
_____7.
'(
Conductiity_ G-0~
302-
-7.9 1
'A-S L'li Temoerature (0C)
"14.1 11______
1 1
11 e
E-40---Awmi-pH (S.U.)
7.5&o
,7-1dr 11 "7.,,'O I -7. 5_T
.1-DO (mg/L)
- 7. P, 22.6%
Conductivity
(
~hos/cm) 1
-7 a-0 11
ý Z85 11 I VA -S I
11-q -1 11-~sq Temperature (QC) pH (U.-**O,_,.'
7 o
5
'76 45.2%
Conductivity
-5 Temperature ('Q
.U A
&-S 2.1 L.'I "Ak,.
PH(..
-7.L 5".0 S-6
-7.5_5r5 72.6%
Conductivity
- ýMoscn z./0 7-13
-20(o Temperature (C) 1A.
"0.1
""-qA 100%
O (mg) 7aO3L Conductivityi 100%
Alkalinity I
Hardness (Ing CaCo 3 /L)
'L,10 400C Temperature ('C)
US.
'ýI.*A L.
DH (S.U.)
'7,= "
II -751 I -~----lI-----~
DO (me/L) 100%
Intake Conductivity (ginhos/c 'm)
Alkalinity (mig CaCO3/L)
Hardness (mg CaCOq3IL)
Final Initial Final I Temperature (°C)
IS. 0 a
a ae Initial Page 35 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I Environmental Testing Solutions, Inc.
Page 6 of 6 I
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Species: Pime 1hales
.romelas Date!
(~..I1-ir\\
Client: TVA / Seauovah Nuclear Plant. Outfall 101. Non-treated Date:
os-lk-10 i
Day I
I Analyst l I
I Concen-Parameter tration I___________
Conductivity (umhos/cm)
CONTROL Non-treated Alkalinity (mg CaCO3/L)
-, W),
`9 11
.1 i.
'o I-33(o 19ý %A Hardness (mg CaCO3/L)
Temperature (*C)
DH (S.U.)
,149ýe
-. sL I"s*
II c.Sj
~
o II -7.
I 1.0 II
ý DO (mg/L) 11.3%
Conductivity (jimhos/c )
,5w.-
0
- 7. f.
308 31q
.7.-
_1, 1
L, Temperature (IC)
S 5-oH (S.U.)
r----*----
i DO (ma/L) 22.6%
Conductivity (jimhos/cm)
.2e" o
-7 c
-7.e_-.__l1 2q'7 Temoerature ("C)
S a
-I f
1 -4 9
I 1
10 I
pH (S.U.)
II -.s* I.-
- I I F.'
I-II-1. -
I 1.7
Žz It..
I DO (mr/L)
I--
.45.2%
Conductivity (gmhoIcm) t.l l
I Tl
- A*
II I
1 7__
Temnerature (00 I
I 6H (S.U.)
41114 II ";s1 I "v.,
II 1,5t I
,St II q,'/8 I
. '7 II I
II-DO (mg/L) r4j. f 1 11 -11.71 72.6%
Conductivity (jimhos/cm)
W2.02 7.8 1
IMP f
%L zI -
219 r.
IF-iemperature=
L t
[
14 v.-1
- s4.
I l
I PH (S.U.)
.1-I 100%
I T
ea(meracr) l.8 Hardness (Mg CaCO3/L TR Chlorine (mg/L) 40.4/.
Ternperature 0OC)
"M1-'17 142 0
6 l.
aDO (mgaL)l-=n=-ialIFi"al.Initia inl i
F Conductivity 400 1 (A Hardness 7
.l*
Temperature ' !C) i 11Z~,
%./
q L Initial Final I
Initial
[
Final Initial Final Initial Final 100%
Intake 1
SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 36 of 99
Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: Tennessee Valley Authority Facility: -Sea uoyah Nuclear Plant NPDES #: TN0026450 Project #:
tS,*
County: Hamilton Outfall: 101 Dilution preparation information:
Comments:
Dilution prep (%).
11.3 I 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information:
Test information:
Organism age:
< 24-hours old Randomiziny template color:
G'epm Date and times organisms were born 05,O-10 Ot.W.T*
1 1%
Incubator number and shelf between:
Il i7-Culture board:
I 0
-I ()
location.
Replicate number:
i2 4
5 i 7i1 19 1
Culture board cup number: I 4
1 IS 1, I
YWTbatch:
1.1o11 1*
Transfer vessel information:
IH = "1 Fit7 S.U. Temperature = "ZS
'°C Selenastrum batch:
O*',A60 Average transfer volume (mL): 10.
O(L6 5 batch I
I Daily renewal information:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake termination time 0
l
\\-3 *4-q 0 (A- -I 1005.to
.0 o1A.Ot 0.4LL,
.S-l10-I
/OOS IQ -01 lo ol.
L A
2 4
J.a-IO 1_'__'
0610-10 i oOI2.., 1, 1o0.0S1" 3
CIS.l-1o
%"e*
t o$-A-10 lm*o k41,0% loos h, 1"i to 6
~
rvc.l.in.f
'mtc 11 riN o'
0
,coJ
-If%.f4 7
S-k 4
Control information:
Summary of test endpoints:
Control-1 Control-2 Acceptance criteria
% of Male Adults:
7" -
07
- 20%
7-day LC~o
" oc7.
% Adults having 3' Broods:
I Qb7.
100?.
> 80%
NOEC I
fc,
% Mortaliy:
- 1, 20%
LOEC
> 10o7.
Mean Offspring/Female:
2&
> 15.0 offspring/female ChV
). I Out
%CV:
4, 7,7
- 1.
< 40.0%
IC2 j
)',001.
Page 3 7 of 99 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09
-t Ai o9 Page 2 of 7 Species: Ceriodaphnla dubia Client: TVA / Sequoyah Nuclear Plant. Outfall 101 CONTROL-1 SurvM Date:
O-1 t-IO ival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Young produced 0
CI 0
)
'0 C)
U 0
0 Adult mortality I.
2 Young produced 0
- 0.
0 I
.J.Io.
e....
Adult mortality I
I %-
3 Young produced 0
T*
0*1oI
'O
'°'I 1 0L° Adult mortality l_. -
I
\\_I-I 4
Young produ ced*0 s Adult mortality I...-
L I
5 Youngproduced C1 X
LI'0 10 1
- 0.
iO 9 1 Adult mortality
\\
X*.
I_'-
\\-
V, 6
Young produced 0 0l 0
0 Adult mortality
%.-. I %,
\\-
k-I_-
7 ]
Young produced 16 1
to v I=
Ik I-.
1 l L Total young produced
.30 2 - I 2
7 1= 2
.3c) 2&
Final Adult Mortality
- 11.
N
\\....I..
k X for 3r! Broods
>4 Y
4 X
>e."
Note: Adult mortality (L = live, D-dead), SB = split brood (single brood split between two days), CO - carry over (offspring carried over with adult during transfer),
IC'oncent ration:
% Mortality:
Mean Offspring/Female:
CONC: 11.3%
Survival and Reproduction Data
___Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Young produced 0
C)
C)
I) a Adult mortality L_.
2 Young produced
______ I___I_
Adult mortality
=
=
o3.
Yung produced C
0
".'1.
e, 0
I01 010 a I° Adult mortality L.
L".-
1-ILI 4
Youngproduced 141 2q 1.4f..
1
.I J1 Adult mortality k_
L-I--
L-I LI__
SYoung produced ID I "
1
[1 10 1'
1 t1o 1
I
' I I1 Adult mortality i -
6 Young produced 0
a 10 0
0
[0 0
Adult mortality TI k_
-- I 1*I*I 7
1 Young produced LI, l
I S
ta I
'S 1
-]
3 1-1$ i(.
Total young produced is 3'
2.-
3 b
3, 3t.--
Final Adult Mortality 7L L.
2 I
Nvora: Adut Mortaliraty (Lk = live, D. - dade), SB* - split brood ksingle brood~ spint between Ptwo days), CO - canr over t(onmpnng carred over wit It~t dlUring transfer).
Concentration:
% Mortality:
07.
Mean Offspring/Female:
13111
% Reduction from Control-i:
- %.1 7.
SOP AT] I - Exhibit ATI 1.2, revision 04-01-09 Page 38 of 99
- m AP 0D7ý Page 3 of 7 Species: Ceriodaphnla dubia Client: TVA / Sequoyah Nuclear Plant. Outfall 101 CONC: 22.6%
Surv1 Date:
r)S-i1eo ival and Reproduction Data U
Day I
2 3
4 S
6 7
8 9
10 2a 3
2 13 4
5 6
7 8
10 j-4..
1 Young produced L)
'C*)
rI C
CI v,
I j 1'
r Adultmortality
[\\.
I..
] '--
t L-2 Young produced C) 0 0
1 0_j 0
0 Adult mortality C.
I I'-
I
.. IL_.
3 Young produced 5
0 0
0 10 0loT l
° Adult mortality L
4 IYoung produced
"\\ I 5I L.
4 l
J_.[
q Jl*
Adult mortality L_
I 5
Youngproduced
'-I
'°1 "0
1b I"
Adult mortality K
K
- j L.
k IAutmra t%.
LYoung produced C) Il CL)o 010 ]o_)
0
)
Adult mortality t1 L._
L.
]
1 L...jL j-t.._
Young produced I I Total young produced b
- c.
ýA
.at 7
-3 to Final Adult Mortality 1
L..
L I
NVote:
Adult mortality (L = five, D - dead), SB = split brood (single brood split between two days), CO - can over (off*ring carried over with adult during transfer).
Concentration:
% Mortality:
07.
Mean Offspring/Female:
I-.3a. '
% Reduction from Control-i:
coNw: 45.2%
Survival and Reproduction Data Replicate number Day "
1 2
3 4
5 6
7 8
9 10 1
Young produced o 0 J
( 0 QL i)
QJC Gp Adult mortality [
Lt 2
oYung produced 0
0 0 0I I010Io io Adult mortality k
_ --I
_. ý_ L-.
E ILI 3
Young produced S&
0 b
I0 0C1 Adult mortality t-_
L CZ 4
Youngproduced
_. I %A[
S 1
IJ.L.l.II SI Adult mortality
\\
\\._C
- k.
L.L*R.7 L-I LJ IL-5 Young produced~dl mraiy '17
'L '-,
10 d 'L I* %
!0E1 5
A utm rait I
k.-
L-6 Young produced a
C>
0 0
Q -
2 l~1 01 Adult mortality
[
U.
L..-. *
= L.._
Young produced I[l
-L CI L+/-)L t
Total young produced
,L 1
i I
- s
,o Final Adult Mortality I
..- I I \\.-
=
k I %-.
I
.£ Note: Adult mortality (L - live, D = dead), SB = split brood (single brood split between two days), CO = can over (offspring carnied over with adult durtng transfer).
Concentration:
% Mortality:
7, Mean Offspring/Female:
I
, 2.
% Reduction from Control-1:
- i 7,
SOP AT 11 - Exhibit ATI 1.2, revision 04-01-09 Page 39 of 99
-a o I'~r1~\\
LZJLJL~2
.5 uSd05.5 Page 4 of 7 Species: Ceriodaphnla dubla Client: TVA / Sequoyah Nuclear Plant. Outfall 101 CONC: 72.6%
- Surv, Date:
,OS-1.-I 0 ival and Reproduction Data Replicate number Day 1
21 3
14 5
6 7 18 9
10 1
Young produced a
cJ>
0 01 0j 0
0 Adult mortality
'_.. _J_'- II__-,-
\\
_ j 2
j Young produced 0
0j" Adult mortality t-i4 U l
1 Adult mortli.
1 t
3 Youngproduced 0O Adult mortality
\\.- -.
Adult mJrtulit I A.-l I
\\.-
i-l I
6 Young produced 0
.O Adult mortality t_ -_~~.
L
~
7 Young produced 7..0 1-1 I
7-0 16 0o 14 IR Total young produced 3\\
4
,,A 3" 1 31 a
as 5,4 Final Adult Mortality
- t.
\\
I
\\-
\\.-- I Note: Adult mortality (L - live, D-dead), SBi split brood (single brood split between two days), CO = can over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
O".
Mean Offspring/Female:
aq.q
% Reduction from Control-1:
q L° CONC: 100%
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
Young produced D)
- 0)
Q) 0 1
)
Adult mortality I
\\_
\\-
k-..
.- I Adult mortality L-I L...-]
L..
L-L-
L-L- "
3 Young produced 0
0 0
0 Adult mortality L -
[
L....
LC:
L_ I L
I L-3 Young produced t.0
" IS I"
S S
Adult mortality t_
00-L-.
5 Young produced Il
+/-i*
L.
I 12 I
I 1-!-i-Adult mortality L-J-
t-V.
___I.Ž-
- =-
6 Young produced~~
0
)
00 0
Q 0IQ QQ0 Adult mortality 1 1__ L.-
L_
I.-
I L_
t L
%L I
- 1 7
Young produced
'OL. 7-270 1,
IS Ii l
Total young produced 37, I-L
- 3.
1 3
7-
.31.
Final Adult Mortality
~1,.
L.-i:
Note: Adult mortality (L. - live, D - dead), SB - splirt brood (single b~roodl split betweenl two days), CO - rannover (offivrnnz carred over with adult durnnQ transfer).
Concentration:
% Mortality:
07.
Mean Offspring/Female:
as.
'1
% Reduction from Control-1:
- z2-9 7.
Page 40 of99 SOP AT1 I - Exhibit ATI 1.2, revision 04-01-09
A 0o g77)I>
Page 5 of 7 Species: Ceriodaphnia dubia Client: TVA / SequoYah Nuclear Plant. Outfall 101 CONTROL-2 Surv Date:
oS-i 1-1I0 ival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
Young produced t
C 0
Adult mortality 2
Young produced 6l l" ") b " 6 0 I" Adult mortality
\\-
I_ \\0 Y
Young producedI Co 0
D o
o1 Adult mortality L
W L_
4 Young produce S*
B l,
t" l
".l Adult mortalityt.___a_
L.
4 Young produced
[
Adult mortality t~I 11-11I
%6 116---
5 Young produced 0~
0 0
0 0
Adult mortalit t___
t...-
t
-\\.-
61 Young produced ro I -&
6
_415 1-.
s i
Final Adult Mortality X for 3r" Broodsi.
L..
"W Note: Adult mortality (L live, D - dead), SB - split brood (single brood split between two days), CO - carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
7 Mean Offsrinf/Female:
I
- m. I CONC: 100% Intake Survival and Reproduction Data Replicate number Day
_1 2
3 4
5 6
7 8
9 10 1I Young produced 1C-010 1
I
- 0) 0 0
Adult mortality L,-
I--I..-
2
[Young produced I
0 I(
3
- 0)
G3I 0
Adult mortality V--.
I l
- L Adult mortality
_ LJ."
U 3
[Youngproduced
,I A
k 5
LA 01 to Adult mortality t:
tI L_
t.--
4 Young produced 1 A
'-1 1 'L 14 S
°
° S
I Adult mortality
%~-1 I'.'-
J
-L 6
Young produced 0
0'A 0L C)ISII~I 4
I Adult mortality L
X--
L_-
L.
L_
7 Young produced 1 1" l 0101 i I T
[ 1-C 1
£L Total young produced
&A I,31
,31 Final Adult Mortality L
in.
tus r
- n. tea. y ov. U ouo pn OO on~
lv pnoreosoOy)
-- I..
a-o---iy over to--oepnog cumea overk d.--
P aatt-urt
.er..
MttI~:
-t*U[ Mortubly] (L - live, D1 - deadi). S
- spllI broodl ksinsle broodt spht[ beltween-two dlays), COu = cern over (onfsonug carred over withl adult during transfer).
Concentration:
% Mortality:
07, Mean Offspring/Female:
a4.
% Reduction from Control-2:
!.7, SOP ATI I - Exhibit ATI 1.2, revision 04-01-09 Page 41 of 99
I l
i T
I i "
- I*
]-
I 0
Environmiental Testing Solutions. Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 11 - 18,2010 Verification of Ceriodaphnia Reproduction Totals
'0'0 Control-i Day Replicate number Total 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 3
.4 4
3 3
3 5
5 5
40 5
9 9
11 10 10 12 10 9
11 11 102 6
0 0
0 0
0 0 0
0 0
0 0
0 7
16 16 15 13 14 16 15 15 14 12 146 Total 30 28 30 27 27 31 28 29 30 L 28 288 11.3%
Day Replicate number Total 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0,
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 4
3 4
5 5
4 5
4 4
42 5
10 11 11 12 10 13 10 12 11 12 112 6
0 0
0 0
0 0
0 0
0 0
0 7
16 19 15 15 18 15 17 13 15 16 159 Total 30 34 29 31 33 33 31 30 30 32 313 22.6%
Day Replicate number Total Dy 1
2 13 4
5 16 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 6
4 4
4 4
4 4
43 5
12 10 13 10 11 11 11 11 11 13 113 6
0 0
0 o 0
0 0
0 0
0 0
0 7
17 15 17 18 16 17 19 15 15 19 168 Total 33 30 34 34 31 32 34 30 30 36 324 45.2%
Replicate number Total Day 1
2 3
4
- 5.
6, 7
8 9
10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 4
4 5
5 4
5 5
5 5
47 5
12 12 10 12 13 10 11 11 11 11 113 6
0 0
0 0
0 0
0 0
0 0
0 7
17 20 18 16 16 19 18 15 19 14 172 Total 34 36 32
.33 34 33 34 31 35 30 332 72.6%
Day 1-i-2"T Replicate number Dy 1
2 3
4 5
6 7
8 9
to Toa 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 6
4 4
5 4
4 5
5 5
5 47 5
13 10 12 11 12 13 10 12 12 11 116 6
0 0
0 0
0 0
0
-0F0 0
0 7
20 17 18 18 17 20 16 19 18 18 181 Total 139 31 1 34 34 33 37 31 36 35 34 344 100%
Re plicate number Day 2
3 4
5 6
7 8
9 10 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
5 7
5 5
4 4
4 5
49 5
11 14 11 12 13 12 13 13 11 13 123 6
0 0
0 0
0 0
0 0
0 0
0 7
19 21 19 20 18 15 17 17 17 19 182 Total 36 39 35 39 36 32 34 34 32 37 354 Control-2 Replicate number Day 1
2 3
4 5
6 7
Total 1
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
3 4
4 5
4 4
3 3
3 3
36 5
11 10 11 11 12 11 13 11 11 1111 112 6
0 0
0 0
0 0
0 0
0 0
0 7
16 13 16 16 14 15 12 15 15 15 147 Total 30 27 31 32 30 30 28 29 29 29 295 100% Intake SRep icate number Day 1
2 3
4 5
6 7
8 9
10 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
4 5
4 4
5 5
5 4
46 5
14 12 11 15 12 14 13 13 13 14 131 6
0 0
0 0
0 0
0 0
0 0
0 7
17 17 18 14 18 16 19 15 18 19 171 Total 37 33 33 34 34 34 37 33 36 37 348
J Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 11 - 18, 2010 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
Reveiwed by:
6155 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from
(%)
1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variaton (%)
control (%)
Control - 1 30 28 30 27 27 31 28 29 30 28 100 28.8 4.9 Not applicable.
113%
30 34 29 31 33 33 31 30 30 32 100 31.3 5.2
-8.7 22.6%
33 30 34 34 31 32 34 30 30 36 100 32.4 6.5
-12.5 45.2%
34 36 32 33 34 33 34 31 35 30 100 33.2 5.5
-15.3 72.6%
39 31 34 34 33 37 31 36 35 34 100 34.4 7.3
-19.4 100%
36 39 35 39 36 32 34 34 32 37 100 35.4 7.1
-22.9 Control -2 30 27 31 32 30 30 28 29 29 29 100 29.5 4.9 Not applicable 100% Intake 37 33 33 34 34 34 37 33 36 37 100 34.8 5.0
-18.0 Outfall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
MSD =
PMSD =
2.085 7.2 1.241 4.2 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10t' percentile) = 13%.
Upper PMSD bound determined by USEPA (90t percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
I IVQ S
SEnvironmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 11 - 18, 2010 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction StartDate:
5/11/2010 Test ID:
.CdFRCR Sample ID:
TVA / SQN101 End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-%
1 2
3 4
5 6
7 8
9 10 Control-I 30.000 28.000 30.000 27.000 27.000 31.000 28.000 29.000 30.000 28.000 Control-2 30.000 27.000 31.000 32.000 30.000 30.000 28.000 29.000 29.000 29.000 11.3 30.000 34.000 29.000 31.000 33.000 33.000 31.000 30.000 30.000 32.000 22.6 33.000 30.000 34.000 34.000 31.000 32.000 34.000 30.000 30.000 36.000 45.2 34.000 36.000
.32.000 33.000 34.000 33.000 34.000 31.000 35.000 30.000 72.6 39.000 31.000 34.000 34.000 33.000 37.000 31.000 36.000 35.000 34.000 100 36.000 39.000 35.000 39.000 36.000 32.000 34.000 34.000 32.000 37.000 Intake 37.000 33.000 33.000 34.000 34.000 34.000 37.000 33.000 36.000 37.000 Transform: Untransformed 1-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean Control-I 28.800 0.9763 28.800 27.000 31.000 4.856 10 32.583 1.0000 Control-2 29.500 1.0000 29.500 27.000 32.000 4.860 10 11.3 31.300 1.0610 31.300 29.000 34.000 5.228 10
-2.742 2.287 2.085 32.583 1.0000 22.6 32.400 1.0983 32.400 30.000 36.000
. 6.539 10
-3.948 2.287 2.085 32.583 1.0000 45.2 33.200 1.1254 33.200 30.000 36.000 5.462 10
-4.825 2.287 2.085 32.583 1.0000 72.6 34.400 1.1661 34.400 31.000 39.000 7.277 10
-6.141 2.287 2.085 32.583 1.0000 100 35.400 1.2000 35.400 32.000 39.000 7.072 10
-7.238 2.287 2.085 32.583 1.0000 Intake 34.800 1.1797 34.800 33.000 37.000 5.032 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.581877172 1.035 0.151372592
-0.51208247 Bartlett's Test indicates equal variances (p = 0.47) 4.581434727 15.08627224 The control means are not significantly different (p = 0.28) 1.105263158 2.100922029 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Duannett's Test 100
>100 1
2.085110834 0.072399682 55.21666667 4.157407407 1.9E-08 5,54 Treatments vs Control-I Linear Interpolation (200 Resamples)
Point SD 95% CL Skew IC05 ICI0 IC15 1C20 IC25 IC40 IC50
>100
>100
>100
>100
>100
>100
>100 sqnlOl_O5llOdata
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 11 - 18,2010 Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/11/2010 Test ID:
CdFRCR Sample ID:
TVA / SQN101, Intake End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-%
1 2
3 4
5 6
7 8
9 10 Control-1 30.000 28.000 30.000 27.000 27.000 31.000 28.000 29.000 30.000 28.000 Control-2 30.000 27.000 31.000 32.000 30.000 30.000 28.000 29.000 29.000 29.000 11.3 30.000 34.000 29.000 31.000 33.000 33.000 31.000 30.000 30.000 32.000 22.6 33.000 30.000 34.000 34.000 31.000 32.000 34.000 30.000 30.000 36.000 45.2 34.000 36.000 32.000 33.000 34.000 33.000 34.000 31.000 35.000 30.000 72.6 39.000 31.000 34.000 34.000 33.000 37.000 31.000 36.000 35.000 34.000 100 36.000 39.000 35.000 39.000 36.000 32.000 34.000 34.000 32.000 37.000 Intake 37.000 33.000 33.000 34.000 34.000 34.000 37.000 33.000 36.000 37.000 Transform: Untransformed
.I-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Control-i 28.800 0.9763 28.800 27.000 31.000 4.856 10 Control-2 29.500 1.0000 29.500 27.000 32.000 4.860 10 11.3 31.300 1.0610 31.300 29.000 34.000 5.228 10 22.6 32.400 1.0983 32.400 30.000 36.000 6.539 10 45.2 33.200 1.1254 33.200 30.000 36.000 5.462 10 72.6 34.400 1.1661 34.400 31.000 39.000 7.277 10 100 35.400 1.2000 35.400 32.000 39.000 7.072 10 Intake 34.800 1.1797 34.800 33.000 37.000 5.032 10
-7.405 1.734 1.241 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93073082 0.868 0.22652909
-1.16236167 F-Test indicates equal variances (p = 0.56) 1.491891861 6.541089535 The control means are not significantly different (p = 0.28) 1.105263158 2.100922029 Hypothesis Test (l-tail, 0.05)
MSDu MSDIp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.241064166 0.042069972 140.45 2.561111111 7.2E-07 1,18 Treatments vs Control-2 sqnlOl_O5J1Odata
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 11 - 18,2010 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test U
KIT' EPA-821-R-02-013, Method 1002.0 Li
[---j)
Daily Chemical Analyses Environmental Testing Solutions, Inc.
Project number.
6155 Reviewed by: r3 m*
m Concentration lParameter Da 0 Da 1 Da 2 Da 3 Da 4 Da 5 ntil FnIn iniia Fia n
Iia Final I Initial I
Final I Initial I
Final I Initial I Final I
ZZZaz 7
a l
nH iSUl
-- r.
a.
DO (m*V/L Conductivitv tumhos/cm)
Control 7.61 7.5 7.59 7.7 8.1 332 336 64 62 94 94J Alkalinity (mgtL CaCO3)
Hardness (mg/L CaCO3) lem ramrett DOra L 11.3%
Conductivi mhos/cm Temperature
- °C) 22.6%
Conductivi hos/cm Tern rature
(°C pH tSU*
DO 45.2%
Conductivi hos/cm Tem erature C)
DO lag/L) 72.6%
Conductivity Otmhos/cm)
Tem ratur¢ C
DO m
Conductivi ho*cm 100%
Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
Total Residual Chlorine (mp.JL)
Tem rature
(*C DO L
Conductivi hos/cm 100%
Intake Alkalinity (mg/L CaCOs)
Hardness (mg/L CaC03)
Total Residual Chlorine Yem rature C
i I_____
emuperature t UI.
Z'+Of'I L'#.Y LQf.Y LQ+Ol L4.01 145 4.5 24.7 4
24.8 25.1 24.81 24.81 PH (SU) 7.54 760
.591 7.561 7.54 60 7.46 76 7.53 7
.6 60 754
.50 4
DO (-g/L) 7.8 79 8.01 7.91 7.9 77
.8 7.676 8
8.1 80 7.6 7.
11.3%
Conductivity (jumbos/cm) 30'1 3('2ý
ý 299 299 30'8 308 314
______Temperature (C) 24.9 25 24.9 25.2 24.8 24.8 248.50 27 25 24 21 24.9 24.9 PH (SLI) 7.56 7
.60 760
.56 7.55 760
.47 7.59
.6 764
.61 75 7.51 76 DO (ni/L) 7
- 7.
9 8.0 7.9 7.9 7
7.8
- 7.
7.7 8.1 810 7.67.
22.6%
Codciiy(mo/m 284
-285 284 282 2~0292.
294
______Temperature(0c) 24.9 25 24.9 25.3 24.81 24.8 248.50 28 249.27 24.8 24.9 2.
pH(SU) 7.56 758
.60 7.55 7.57 760
.47 759
.56 764
.59 7.541 7.52 76 5.% DO (niWL)
- 78.
8 8.01 7.9 8.0 7
.8 78.7 8
8.2 82 01 7.7 7
4.% Conductivity (ginhos.Icn) 253 256 25322 256
-257 258 T_____
emperature CC) 25.0 25.0 24.9 25.1 248.
25 248.
4928
- 24. 8 24.7 25 24.9 24 pH(SU) 7.56 7.59 7.58 7.56 7.55 758
.46 759
.56 763
.60 752
.51 76 DO (mgL) 7.8 7.8 8.0 7.9 8.0 7
.8 78.8 8
82
.2 81 7.8 7
72.6%
Conductivity (jznhos/cm)'
210 213 206 202 26 212 214
_______Temperature (IC) 25.0 25.0 24.8 25.1 24.8
- 2.
24924.9 24.8 24.9 24.8 25.0 24.9 2.
PH (SU) 7.55 7.58 7.581 7.5 7.54 75
.6 75
.5 76
.7 75
.2 76 DO (mg/L) 7.81 7
8 7.81 8.0 7.8
- 7. 7.8 7.81 8.21 8.21 8.1 7.97.
Conductivity (uunhos/cm) 166916716161819 100%
Alkalinity (mg/L CaCO3) 6 26 Hardness (mgf1L CaCO3) 6 76 Total Residual Chlorine (mgIL)
<01<.0<00
_______Temperature (OC) 25.11 25.11 24.8 24.9 25.0 24.9 24.91 24.9 24.91 24.71 24.81 25.11 25.11 5.
pH (SU) '7.551 7.571 7.591 7.54 7.51 7.46 7.47 7.56 7.571 7.60 7.571 7.511 7.521 7.60 DO (mZ/L) 7.81 7.8 8.0 7.9 8.1 7.7 7.9 7.7 7.91 8.21 838.
7.8 Conductivit (mho/n 16417 162 6
6 6
7 100% Intake [Alkalinity (mg/L CaCs) 6 86 Hardness (mg/L CaCO3) 7 67 Total Residual Chlorine (In2IL)
<01<.0<.0
______Temperature CC) 25.11 25.31 25.01 24.9 25.11 25.11 24.9 24.8 24.91 25.01 24.8 25.01 24.81 25.0
- A 0
o 'T11z Page 6 of 7 Species: Ceriodaphnia dubla Client: TVA / Seauoyah Nuclear Plant. Outfall 101 Dailv Chemistrv:
Date:
oa-It-1 0 Y
Day 0
1 2
Analyst 1W4 11 /W 1
0 11 A CS I I~L*
II Concentration I Parameter DH (S.U.)
- I-Gq II -7.(a, I 7. q1.
II -7.5(4 I -7.45 II
-LtI t -~------I DO (mg/L)
CONTROL Conductivity, (jgmhos/cm)
Alkalinity (Mi CacofL)
Hardness (mg CaC0 3/L) 1 32-6.
IN (oQ
-7* -
Iý -7. 9'(,
7.!7Y V Temperature (0C0 I
I.
pH (S.U.)
DO (mg.L) 11.3%
Conductivity (jmhLm
-7 q
-1.
1 7. q 11 13--4 zt OI Temperature (°C)
U oH (S.U.)
"I.S6 II -7.(,o I 1-t.1#o II -7.'ChA I -7.47q II II ii-DO (mp/,)
-'14 22.6%
Conductivity Temperature (°C) a a
oH (S.U.)
Z85
- 7.
.o 5-7 LS.G
-W.9 I.
I L
1.o r
I
'2 DO (mY/L) 45.2%
Conductivity Temperature (0C) a h ~
I I
II I
II pH (S.U.)
I.
I 5,
1-7, 6s.7r DO (mg/b) 72.6%
Conductivity 1
"i1' I
U Temperaturel -*T pH (S.U.)I DO (me/L)
I 1.8 II
- 7.
I 100%
Conductivity (ginhos/m Alkalinity (Mg CaCO3/L)
L..
Hardness (Mg CaCg 3 /L)
LA
1O 4 0.j 0.1
- 7.L.
I I
-Lt~.tD I
',.A1 I
Temperature (°C) ts. I
- l I
L-'1.1 I
a a
nH (S.U.)
-A.& 5 9 DO (mL.!L) i
[I 100%
Intake Conductivity (gmhos cm)
Alkalinity (mg CaC 3 L)..
Hardness TMg CaCo 3/L)
'O. ID 1Vz O".O WoIt Temperature (°C0 1~s h
I ~
II Final I
Initial II Final I
Page 47 of 99 SOP AT 1I - Exhibit ATI 1.2, revision 04-01-09
0 D
Page 7 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequovah Nuclear Plant. Outfall 101 Date:
.tk-IM I
I 1
I Dav 1
Da I-.
3 I
4 I
5 I
L~ 6 II u
ii
~.
I II
-nays Concen-Parameter tration
-- i I
pH (S.U.)
DO (mg/L)
CONTROL Conductivity (Rimhos/cm)
Alkalinity
[
(mg CaCr 3/L)
Hardness (mg CaCQ3IL)
[
Al I.Mt
+.I 1
t.* *G
,,~*
-O l
\\ *two
-,1 LCI r-Lq Temperature (0C0 ii I
II I
pH (S.U.)
" II " o I 1.
II
-A.
i I.
II "7. -'"
I "7.*'
II-;
V DO (rag/L)
II 11.3%
Conductivity I
(iimhos/cm) 31 L/
7-q Temperature (°C)
I I --
I I
0 8 PH (S.U.)
I " t.*,, II T W I imi II -7. S I "7.S 1 II 7.(2. I I*
~l.
DO (mWL)
'Ji'I 22.6%
Conductivity V.
V-el 0 Iq /m zqJ LJ Temperature (0C)
U P ~
nH (S.U.)
Q 1.rt II isgq I 7.so II "l-.
I 7-sn II 2--s I -1 S-II I
II I
II I
DO (ma/L) 45.2%
Conductivity (iimhos/cm)
'4.
1.
1 1
1"*,
2-ý.(;
-2 "Q.T'.Z
,A-M V.1.
.7 Z5~3 24 Temperature ('C)
",t,q I
a h ~
I II i'[I I
II f
II 1"1 1
nHl (S.U.)
"*.q(z II pH (.....
l----.
DO (ma/L)
.5. -4i-~--
72.6%
Conductivity (iumhos/cm)
_ s
_JI
--Ko) II" 7'- ! 7
ý.ST II 7.
1-3 -
i.1 7-Temperature (0C0 11==
09 4
pH (S.U.)
.3 1--
CJCI
_.J DO (mgfL) 4 II 7,57 I -76e-ILL 100%
- /-
CO, 10 Temperature (°C)
I "L'L4 v-9.T II nH (S.U.)
"r-i---
1
- i.
1-ii 1
_I H-DO (mg/L)
~-
100%
Intake Conductivity AlkalinityI Hardness TR chlorine (mg/L)
Temperature (°C)
IS7~
(to 0.10 f-o4.-
44 l
II I
L'I.E II '.'U I
7-i.*
II 4.3.U I
m I
L14-9 1
1-5 0
114.
6 Initial Final I
Final I
Initialm-1 Final I
Initial I
Final 1
Page 48 of 99 SOP AT 11 -Exhibit AT 11.2, revision 04-01-09
S 0
atD~T~
.~1 Envimnmenta Test tig SoIu ens lust Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas Client: Tennessee Valley Authority Facility: Sequoyah Nuclear Plant NPDES #: TN0020168 Project #:
toISS County: Rhea Outfall: 101 I
I U
I Dilution preparation information:
Comments:
Dilution prep (%).
11.3 22.6 45.2 72.6 100 Each concentration was UV-treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes toremove pathogenic Diluent volume (mL) 2217.5 1935 1370 685 0
Interferences.
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
2,. %
ik"s Gub Randomizing template:
I LOe..
Date and times organisms OS -
-10 (000 Incubator number and were born between:
shelf location:
Organism source:
(ctM 6t-r p
,7 Artemia CHM number:
CR$.I11 Drying information for weight determination:
Transfer bowl information:
pH =
&I S5 S.U.
Date / Time in oven:
0
'e0 Temperature =-6,\\C Initial oven temperature:
Average transfer volume:
Date / Time out of oven:
_-__%__o 0,
Final oven temperature:
60"C.
Total drying time:
"I_-___
i 1%so i
Daily feeding and renewal information:
ii ii Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0
05~ l-O 11 1 1 160 0 1 zo
- FS Al o o sg IV. 0%
1 o risI$
io.o ¢..
O -CA-lo 3
0S-1q-/I oDbo
_I Io I -Otl1 toslQ.06 1oo06a.
1o.1j1 4
05.i0 OV*
'33 o I118 -L..
taostq.oi
~oS,q&. 10 tG1,1-tO ii II 6
06 - l-'Q0 i
utlos4.oq IoagN s L 1
ii 7
I~/
Control information:,
Acceptance criteria Summary of test endpoints:
% Mortality:
0".
<20%
7-day LC50
>I o07.
Average weight per initial larvae:
- 0. oi NOEC C 0ol.
Average weight per surviving larvae:
- 0. R'"1
_ 0.25mg/larvae LOEC
" 1007, ChV IC2 5
-T
> 1667.
I Page 49 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
0 A
Environmental Testing Solutions nC Page 2 of 6 U
Species: Pimephales promelas Client: TVA / Seauovah Nuclear Plant.II Outfall 101, UV-treated
'Date:
OS.- I k-Survival and Growth Data U
I U
I I
I I
I I
I Day CONTROL 11.3%
22.6%
UV-treated A
B C
D E
F G
H' I
J K
L 0
10 1o U o 1
(
10 (0 /0 113I(0 1t
/00001 10 to 101 0
1 to 2
/
1 0
1
/0 o 10 10
/0 10
/O 4j I0 (
O /0 10
/0
/0
/0 Io
/0
/o
/0 SI10 10
/0 10
/0
/0
/0 D0 (0
10 /0 j0 lO10 I0O (0 I I01/0I0 t(
l(
lO
/0 6
IID 10 1 r
1 10 C10 tO
/0 10 to/0 10 to 1o 10 to t to /
A f Pan weight (mg)
Tray color coe::\\A Analyst:
i
.1ý 13.*
Aq.10 lZ153 14.10
- 12. -1, H.Q N
13' 13.q ft*'7 13.,5
)q-31 i1tvo Date: Or,. 0')*'10 B = Pan + Larvae weight (Aag) 7yt1BI 2.3.M*.011 22-72.
2.02 2352. M5.35, 23.13 V2.232 l.I "23.3cr 1.02.
Analyst: Uno*[
Date: 0S 10 C = Larvae weight (mg)
=B-A e.7%t. e,1i 1,'4 vlb-t q-k, 8,t,.1%
8-44
,C o I-o1,6= "l.
-Ds0 1-4'L Weight per initial number of larvae (mg)
= C / Initial number of larvae
'V I'.
10' K>
CF"I ra.
'~0 0*
0 0'
6'0 V
4 Average Percent weight per reduction initial from
- 0. 2C1 number of control (%)
larvae (mg)
-. 6104 -
I 5
m o
m17 o~q-jo gT J.*
Icll.
I I
I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
j Page 50 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I I
SEnv rmnmental Testing Solut ens Inc.
Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. UV-treated Date:
OS-I%- )Q)
Survival and Growth Data Day 45.2%
72.6%
100%
M N
0 P
Q R
S T
U V
W X
0 it)
/0 10 10'
/D 10
/0 10 16-10
/0 10Q I
/0
/0 10 10
/0 (0
to /0) to 10 to 21
/0 to
/0 (0
1Qo o
0 t
0 0
t/ 10 23l0 1
0 I0 O1 U
1 0
/0 O 0
'0
/0
/0
/0 4
/0,
(
0 t
0 10 0
t t
b to
(
D0 t/ Io
/
0 5
10
/0 (0
10 D0 (0
/0 10 10
/t 10
/t 6
t(
/0
/0 1
10
/6
/0 t0
- 1) 16
'0
/0 7
1ID 10 1
1 1
10 ID 100 tO
/0 0
00 A = Pan weight (mg)
Tray colorcode::
\\A,.1 Analyst: i ý'Y 3 \\Ll
.3O '2.o t 13
.LF IZ,0t l3.q1 11.ý3
\\Z,1 is.5"9 Date: %*,
5--V). \\nd B = Pan + Larvae weight (mg)
Analyst:
22.*? 2V41 25io It-Si Date:
C5-2'2-tO C = Larvae weight (mg),
=B-A Weight per initial numberooO.
= C / Initial number of larvae o"
Average Percent weight per reduction initial from control 0,98
- 0.
- 0. &
6 A number of
(%)
larvae (mrg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, n = missing, sk = sick, sm = unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
I i
I I
Comments:
SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 51 of 99
Page 4 of 6 Species: Pimephales Dromelas Client: TVA / Sequovah Nuclear Plant, Outfall 101. UV-treated Date:
0j"1kU10 Day 100% Intake Y
Z AA BB 0
/D~
10 1C I 1
10D (0
t t0 2
to
/0 10 S3 t o t t
1 4
6 0
1(
(C) to) 7 t0 1 0
6 A = Pan weight (mg)
Tray color eo~e::
Analyst: iW
,5 1
i.7 Date: O.-. r"7, it B = Pan + Larvae weight (mg)
- 7.
-4
-1325 Analyst:
31 2813 Date:
QG-22-l1 C = Larvae weight (mg)
=B-A Weight per Initial number of larvae (mg)
C / Initial number of larvae 0o Average Percent weight per I reduction initial from control C). &O-L numberof
(%)
larvae m Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
[
Comments:
Page 52 of 99 SOP AT20 - Exhibit AT20.3, revision 04-01-09
Environmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 11 - 18,2010 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
6155 Reveiwed by: ;
r Not for Compliance Assessment, Internal Laboratory QC Cooeantriaio (%)
Repekute 1aiidMamtberit lerf.alnumbewlarel A=Pa weight B=Pan+ Lo ae LaewvgSS ahtl Weight ISuriving Me, wmight/
Caeflekidaevarialtoa WeightIliuial namke meaatnvival Met nweight Coefadient of Perveet redaelionafro larvue
(.We weight (ra)
-A-B numberotlareto(ig)
S.mvhg naaumbero OI u
dOlarve (m.)
(%)
lnitaldnberof vtriatlon o-m coatrul (%)
lrame (ing) a.*'*')
%)
.lvame (rate)
.*m.
A 10 10 13.06 21.28 8.22 0.822 0.822 Control B
10 10 14.10 23.89 9.79 0.979 0.897 9.5 0.979 100.0 0.897 9.5 Not applicable ot 10 10 12.53 20.77 8.24 0.824 0.824 D
10 10 14.10 23.72 9.62 0.962 0.962 E
10 10 12.66 22.02 9.36 0.936 0.936 F
10 10 14.91 23.52 8.61 0.861 0.861 11.3%
G 110487 238.80480.904 6.5 088100.0 0.904 6.5
-0.8 G
10 10 14.87 2.58.48 0.948 0.848 H
o 0o0 13.43 23.13 9.70 0.970 0.970 I1*
10 1 0 14.92 22.82 7.90 0.790 0.790 J
10 10 13.55 21.41 7.86 0.786 8.6 0.786 0.881 3.06.4 100.0 0.881 3.0 10.1 2.%
K I 0 10 14.31.*
23.36 9.05 0.905 0860.9050.6BA1.
L 10 to 13.60 21.02 7.42 0.742 0.742 M
10 10 14.46 23.26 8.80 0.880 0.880 452%/.
N 10 10 16.23 24.68 8.45 0.845 0.881 3.0 0.345 "100.0 0.881 3.0 1.8 0
1 0 1 0 14.97 23.85 8.88 0.888 0.9888 P
10 10 14.30 23.39 9.09 0,909 0.909 Q
10 10 12.08 20.72 8.64 0.864 0.864 R
10 10 13.66 22.41 8.75 0.875 0.875 S
10 10 14.40 23.10 8.70 0.870 0.870 T
10 10 12.90 21.51 8.61 0.861 0.861 U
10 10 13.47 21.58 8.11 0.811 0.811 V
10 10 14.73 23.47 8.74 0.874 0.874 W
10 10 12.87 20.95 8.08 0.808 0835 3.8 0.808 X
10 10 15.57 24.03 8.46 0.846 0.846 Y
10 10 14.74 23.15 8.41 0.841 0.841 100% Intake Z
10 10 14.25 21.90 7.65 0.765 0.802 4.0 0.765 100.0 0.802 4.0 10.6 AA 10 10 13.27 1
21.18 7.91 0.791 0.791 BB l0 10 14.45 22.56 8.11 0.811
_0.811 I
OutfaII 101:
Duonett's MSD value:
PMSD:
intake:
Duannet's MSD value:
PMSD.
0.0916 10.2 0.0887 9.9 MSD =
Minimurn Significant Difference PMSD =
Percent Miniumn Significant Difference PMSD is a measure of test precision. The PMSD is the mininuirn percent difference between the control and treannent that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) - 30/.
Lower and upper PMSD bounds were determined from the I 0th and 90th pereentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 20 01a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821 -B-01-005. US Environmental Protection Agency, Cincinnati, OH.
_I 0
3 EnvironmentalTesting Solutions, inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 11 - 18,2010 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/11/2010 Test ID:
PpFRCR Sample ID:
TVA / SQN101 End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
UV-treated Conc-%
1 2
3 4
Control 0.8220 0.9790 0.8240 0.9620 11.3 0.9360
.0.8610 0.8480 0.9700 22.6 0.7900 0.7860 0.9050 0.7420 45.2 0.8800 0.8450 0.8880 0.9090 72.6 0.8640 0.8750 0.8700 0.8610 100 0.8110 0.8740 0.8080 0.8460 Intake 0.8410 0.7650 0.7910 0.8110 Transform: Untransformed
]-Tailed Isotonic Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean Control 0.8968 1.0000 0.8968 0.8220 0.9790 9.528 4
0.9003 1.0000 11.3 0.9038 1.0078 0.9038 0.8480 0.9700 6.504 4
-0.184 2.410 0.0916 0.9003 1.0000 22.6 0.8058 0.8985 0.8058 0.7420 0.9050 8.644 4
2.394 2.410 0.0916 0.8513 0.9456 45.2 0.8805 0.9819 0.8805 0.8450 0.9090 3.026 4
0.428 2.410 0.0916 0.8513 0.9456 72.6 0.8675 0.9674 0.8675 0.8610 0.8750 0.720 4
0.770 2.410 0.0916 0.8513 0.9456 100 0.8348 0.9309 0.8348 0.8080 0.8740 3.755 4
1.631 2.410 0.0916 0.8348 0.9272 Intake 0.8020 0.8943 0.8020 0.7650 0.8410 4.003 4
Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.968467712 0.884 0.361535501 -0.39091302 Bartlett's Test indicates equal variances (p = 0.02) 13.71642971 15.08627224 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
0.091605544 0.102152823 0.005745267 0.002889611 0.129300907 5,18 Treatments vs Control Linear Interpolation (200 Resamples)
Point SD 95% CL(Exp)
Skew IC05 ICI0 IC15 IC20 IC25 IC40 IC50 21.680
>100
>100
>100
>100
>100
>100 sqn 101_O5111Odata-uv
E SEnvironmental Testing Solutions, Inc.
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated May 11 - 18, 2010 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/11/2010 Test ID:
PpFRCR Sample ID:
TVA / SQN101, Intake End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
UV-treated Cone-%
1 2
3 4
Control 0.8220 0.9790 0.8240 0,9620 11.3 0.9360 0.8610 0.8480 0.9700 22.6 0.7900 0.7860 0.9050 0.7420 45.2 0.8800 0.8450 0.8880 0.9090 72.6 0.8640 0.8750 0.8700 0.8610 100 0.8110 0.8740 0.8080 0.8460 Intake 0.8410 0.7650 0.7910 0.8110 Transform: Untransformed 1-Tailed Cone-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD Control 0.8968 1.0000 0.8968 0.8220 0.9790 9.528 4
11.3 0.9038 1.0078 0.9038 0.8480 0.9700 6.504 4
22.6 0.8058 0.8985 0.8058 0.7420 0.9050 8.644 4
45.2 0.8805 0.9819 0.8805 0.8450 0.9090 3.026 4
72.6 0.8675 0.9674 0.8675 0.8610 0.8750 0.720 4
100 0.8348 0.9309 0.8348 0.8080 0.8740 3.755 4
- Intake 0.8020 0.8943 0.8020 0.7650 0.8410 4.003 4
2.076 1.943 0.0887 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93714929 0.749 0.035344625 -1.47582984 F-Test indicates equal variances (p = 0.14) 7.08368349 47.46722794 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.08868433 0.09889526 0.017955125 0.004165792 0.083183944 1,6 Treatments vs Control sqnlOlO51ilOdata-uv
/.
TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated May 11 - 18, 2010 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-82 1-R-02-013, Method 1000.0 T ý 7 m.
Daily Chemical Analyses Environmental Testing Solutions, Inc.
Project number.
6155 Reviewed by.:
Concentration Parameter D
Da I Da 2 Da 3 D
Da 5 Da 6 Initial Fia Initial Final Initial IFinal IInitial IFinal Initial Fnl initial IFinal Initial IFinai Fin Is PH (SU) 7.56 7.0 7.621 7.481 7.551 7.521 7.601 7.54 7.60 7.9 7.631 7.491 7.521 75 DO (mg/L) 78 8
7.9 7.7 7.9 7.9 7.8 7
7.8 8.1 7.9 8.1 Conductivity (punhos/cm) 318 317 Control Alkalinity (mg/L CaCO3) 62 60 Hardness (mg/L CaCO3) 88 94
_Temperature CC) 24.8 24.8
- 24.
24.7 24.5 24.8 24.3 24.9 24.9 24.7 24.9 pH (SU) 7.60 7.8 7.63 7.44 7.571 7.52-7.6,5 7.60 7.9 7.64 7.9 7.52 75 DO (mg/L) 7.9 7.9
- 7.
9 7.8 8.2 8.2 11.3%
Conductivity (Iunhos/cm) 301 305 300 300 304 309 307 lTemperature CC) 249 24.8 24.8 24.9
- 24.
9 24.9 24.8 24.9 pH (SU) 760 7.58 7.63 7.44 7.56 753 7.61 7.60 7.64 7.52 DO (mg/L) 79 7.7 8.0 7.7 7.9 7.8 80 7.9 8.2 8.2 22.6%
Conductivity (uzmhos/cm) 282 274 282 282 289 296 2
Temperature CC) 24.9 24.8 24.8 24.6 24.9 24.7 249 25.0 24.9 24.8 24.9 pH (U) 7.61 7 58 7.63 7.44 75 7.5 3
7.61 7.5 79 759 7.65 7.481 7.53 75 DO (mgL) 8.0 77 8.0 7.6 7
7.9 8.0 76.9 80.3
.7.81 8.27 45.2%
Conductivity (Imhos/cm) 2 259 24 251 256 257 FTemperature(C) 249 24.59 246 250 24 29 25.0 2449 2469 PH (SU) 761 7
762 744 756 7561
- 7.
60 7563 7451 7.% DO nsalL) 8.0 77 8.01 7.6
- 79.
9 80.7 0
8
- 0.
8.3 788.37 72.6%
Conductivity (mnhos/cm) 209 2
208 214 219 Temperature C 249 24.5 249 245 250 245 249 250 2479 2469 pH (SU) 7.58 7.52 7.61 7.4 7.55 7.51 7.58 7.60
- 7.
61 746 7.50 DO (mg/L) 8.01 7.71 8.11 7.7 8.01 8.01 8.11 7.6 7.9 8.131 7.81 8.37.
Conductivity (pihos/cm) 71 100%
Alkalinity (mg/L CaCO3) 62 Hardness (mg/L CaCO3) 7 Tem erature CC) 25.1 24.7 24.9 24 25.2 24.5 25.0 24.6 25.0 24.6 250 246 25.0 24.5 H (SU) 7.58 7.55 7.62 7.4 7.53 7.52 7.59 7.52 7.58 7.56 7.60 747 7.50 7.55 DO (mgtL) 8.0 7.7 8.1
- 7.
8.1 7.8 8.1 7
7.8 8.1 83 79 8.3 7.8 Conductivity (jumhos/cm) 100% Intake Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
_Tem rature C 25.0 24.6 25.0 2
25.1 24.6 24.8
- 24.
24.9 24.3 24.8 24.6 25.2 24.6
0
~
Enlromensl~stlg Slutioms Inc.
Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. UV-treated Daily Chemistry:
Date:
I- 0 I
Day I
I Day Analyst Concentration JParameter U
U I
I U
Conductivity (umhos/cmi' CONTROL UV-treated
- r 4
Alkalinity (ma CaCO1L)
I
~
-L*et tx
-J 7.q 32o03 S--
4 Hardness (mI CaCO/li TemDerature (00C pH (S.U.)
- 7. (aD 1110wý
-7 TA 1 7-1o3 11 -7. L/ Lf I 7.,q7 11 I
II
[
I
- I II I
DO (mg/L) 11.3%
Conductivity (mhos/cm) 1 *- 18 L1 4
-77 11¶ Temperature (QC)
U U
oH (S.U.)
"7.(#O L7.-
7.4*3 I W I
.t L
IIB E
f I
DO (mg/L)
" 22.6%
Conductivity I e 1*0Z7-'
Yl iZ 0,
zwVA A
Temperature ('C)
S 6
~
pH (S.U.)
7.IM,3 4 ~
DO (m&L)
P30 II "1,
45.2%
Conductivity (AMmos/cm)
-0' 7
I 12q I'A M.6 Temoerature (M)
I I
I II I
1 II I
II PH (S.U.)
7.6
--7.-5-o
-,-I I -l.,z 1! "*ý 0
i5-77, 72.6%
Conductivity
~
q2C Temiperature (*C)
-1q-
'W4 9
AS 1
o.
DO (m PL)
~
,OiI 7
I i
100%
Conductivity (imhos/cm) 1_70 Alkalinity (mg CaCO3IL)
Hardness (mg CaCO3i/L)
TII chlorine (mg/L)
~.I e40 I cý Temperature (MC) 7.1,
-- *'i II I
I k
I "
fl g
Ire S ~
S -
pH (S.U.)
-7, (.gZ
'7-5' 3 DO (m~iLl II
'-7.-i 100%
Intake Conductivity (gmhos/cm)
I(2P7 Alkalinity (mg CaCO3 _L)
Hardness (mg CaCOAL)
TR chlorine (mg/L) c-0. I0 Initial Final Initial Final SOP AT20 - ExhibitAT20.3, revision 04-01-09 I
I Temoerature ( 0C) 2&.0 11 D.
Initial Page 57 of 99
-a I
I I,
I I
I I
I I
0 igSltonDIc Page 6 of 6 Species: Pimephales promelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. UV-treated Date:
r~~...II-iD I....
l'.
I Dav I
Analyst F 3
.rlli I
4 I
5 I
611 OL
.9 Conce-n-IParameter t tairation pH (S.U.)
DO (mg/L)
Conductivity (timhos/cm)
CONTROL UV-treated Alkalinity (Mg CaCO3IL) 3I l
1 IN II x.,t.*.
ILAq%
I,t*
Ia~t I-q Hardness (mg cacO3/L)
Temnerature (0C0 PH (S.U.)
-u.5
.iO 4
-7. s-z
-7.
DO (mg/L)
"7.
11.3%
Conductivity (jimhos/cm)
Temperature (C)
Iq 911 I.
pH (S.U.)
'7.
- 0 0
l4
.a
-zsq DO (mg/L) 19-.
22.6%
Conductivity 19 Temperature 0C)
-A-S 113 pH (S.U.)
II 1.S I 59 II~I-57 I
II 7Y8 I7.5 "It 7SYo DO (mc/L) 45.2%
Conductivity (limbos/cm) 5IJ "A"
-LA A Temperature (0C "t.I.c binJ MIS
- Z St.
9 I --
I II I
pH (S.U.)
r4Z'
-114A 1 WO 11 -4.5% 1 q--to-3 11 DO (mg/L) 1 8-5 Ir 0 1 00, 72.6%
Conductivity (Amhos/cm)
Temperature (°C)
N.4.
7.tb.o
-4.5l L*.t I
-. 6, t
7, 7A
- 0 II "qm S
SE 1)H (S.U.)
DO (me/L)
I I
100%
Conductivity (jnnhos/cm)
Alkalinity (Mg CaC0 3/L)
Hardness (mg CaCO3/L)
Temperature (0C)
(02-
- m. Io aO 79.
111 a PH (S.U.)
DO (mg/L) 11 14,16I 1 L... 0 IiTU I
I I
100%
Intake Conductivity (pmhos/cm)
Alkalinity (mg CaC. 3/L)
Hardness (mg CaCQ3IL)
Temperature ('C)
Ei~E
- ~~ll -
a I
I
.I I
7A I
i L
1 M LI*
I
.,%.'L L*-.
V-Initial Final Initial Final Initial Final IInitial Final SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 58 of 99
1 II K
K' K
Si Page q___"_
Page j of J___
a SEnvironmental Testing Solution% Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst F/.t*iWa7 Date analyzed C6*I" J C> I I odide reagent:
Acid reagent: [
33'1 Calibration:
1 0.10 mg/L _
1.00 mg/L Slope Reference standard number 7
so.'[.
Note: For samples with a residual chlorine of > 1.0 mgrL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
i!
II i
I I
I I
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L) r (acceptable range = 90 to 110%)
nos$ 1
- 0.50 Duplicate sample precision:
Sample Sample EiD Sample characteristics Residual chlorine
%RPD = {(S - D) /[(S+D)/2]) x 100 number m/)(cetberne 1%
oo611.c4 CQacf o$2c w f ?,1AW sto.0o3, Z.
Duplicate D 4'0 oZ4'2,C
(
t-5 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mgi'L)
Reagent Blank 00Q6 Z' F.51I~
bI)O.°°°(
~ 4
~_____
100610-03 AK4' VA)
&PI 00T 0A~
603-3 I
-) 1.
1 Note, All samples were analyzed in excess of EPA recommended holding time (1'3 minutes) unless otherwise noted.
Laborator" control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVx 100 number (mg/L)
(mg/L)
(acceptable range =90 to 110%)
[:;:-I
ýP 0.50 0
- o.
f.*
/
- 3.
j Reviewed by 6hZZZ l Date reviewed U-3 I
SOP C8 - Exhibit C8.1, revision 09-01-09 Page 59 of 99
Page Page j of J EnvironmentalTestIng Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumnet Model AR25 pH/Ion Meter Analyst L 7 I Date analyzed [i.I, I I Iodide reagent: uA1*i IZI Acid reagent: I w S bj Calibration:
mg/L Reference standard number I I0.5I105 m
I/ I 1.00 -pa
-7 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
- 0. 50 oso
- 0.
-0.
Y Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine I%RPD = [(S - D)/[(S+D)/21] x 1001 number (mg/L)
(acceptable range = i 10%)
w5613.Corf1 0
AorJt1ar (6i 9 S L 0o.02-'-q Duplicate Dp o
o.o.
1 -
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number
_m_/L)
Reagent Blank
- 10.
0.034 T;X u,00J
$P CAu,-1 I
,a
.cc033o 160ý-5 rt. V IS
.A0 1 q\\
'~,drliPIO0.
0002q W
~Y2= 13:
\\WC1
?Ale.A-dV Lff_
Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS =MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
- 1) -
0.50 4o
-I 1o
./. (6 Reviewed by I 6 0 V
[
Date reviewed
'I Q,
A1
. J SOP C8 - Exhibit C8.1, revision 09-01-09 Page 60 of 99
Page i51 Page o off__
E Environmiental Testing Solutions Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter ii Analyst
[146-1-1 Date analyzed Calibration:
J Iodide reagent:
IN 1 0
I Acid reagent:
w P-39 I
1U 0.10 m =/
1.00 niglL I
Slope Reference standard number a145-Ad I 1A 1,
Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
145Sbe)0.50*L Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = [(S - D) /[(S+D)/21) x 100 number (mgiL)
(acceptable range = E 10%)
Duplicate D 41-4,
ý Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number bme/L_
Reagent Blank I ws 11A.0~ k a2 Fe a r 03
.fi r
lesxq, -* CSu n-, tzo albr, 800.W 7
V.TO I.lobsira La" 447Qof2JJ.
~
~
Z Note: All samples were analyzed in excess of EPA recommended foldingtibe (15 minates) unless otherwise noted.
Reference standard True va Measured value
% RS = MV / TV x 100 number (acceptable range= 90 to 110%)
0.50 Reviewed by I A
Date reviewed O
tS -I SOP C8 - Exhibit C8.1, revision 09-01-09 j
Page 61 of 99
S Environmental Testing Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst E*.iII I
Date analyzed [__
Calibration:"
Page 5
Page -
of-_3 odide reagent: L Acid reagent:
I II I
ii II~
Reference standard number Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
II
[
L I
ij Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (rag/L)
(mg/L)
(acceptable range = 90 to 110%)
I Ao 0.50 tq, Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = [(S - D) I[(S+D)/2I) x 100 number (mg/L)
(acceptable range = -10%)
I Duplicate D to.
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number Reagent Blank I
I 0.0O I, oovsII iee A jf9iVb c
gaýv 0O.X 134O U
-0 g,
i~~.q 0a
"*o!
6eo.ooq~z 14 s4
- iAWL, 44a I'
_____r43_062 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Ii Ii Reviewed by I i
=
Date reviewed L itS J
- /0 SOP C8 - Exhibit C8.1, revision 09-01-09 Page 62 of 99
Page,6__5 Page __&
of 0
9
.'.Environ mentai Testing Solutions. Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed j l,
10 I
Iodide reagent:
Acid reagent:
Calibration:
R t
0.10 mg/L 1.00 mgfL
- Slope, Note: For samples with a residua" rfe ýof> 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorv control standard:
Reference standard True value
)
Measured value (MV)
% RS = MV/TV x 100 number (mg/L)
(mg/L)
(acceptabli range = 90 to 110%)
I 0.50 Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorin
%RPD =
D) /[(S+D)/2]} x 100 number (mIL)
(acceptable. range 10%)
S Duplicate D
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
Reagent Blank 4,"
N-,:Ta Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MV/TVxI00 number (rag/L)
(mg/L)
(acceptable'range = 90 to 110%)
5S(a6 0.50 Q,4 Reviewed by I A
Date reviewed I O*ll I 0 Page 63 of 9-9 SOP C8 - Exhibit C8.1, revision 09-01-09
Environmental Testing Solutions, Inc.
Page l1I-Page k
of 6 Alkalinity (SM 2320 B)*
Matrix: Water, RL = 1.0 mg CaCO3/L Analyst I &;([
Date analyzed I ID Time initiated leh, e* 1lc0 Time completed 813 Titrate samples to pH = 4.5 S.U.
Tifrant normality and muIIbli~r dpl,,rminnane..
pH of Normality TNormality (N) of HSO4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml Na 2CO3 x 0.05)/E
= (Nx 50000)/100 ml sample water reference standard ml ml ml
=0.251E
=Nx 500
=_4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
%wl C-r: bo?0.1)
- 0
-0.m%
Laboratoly control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL)
(acceptable range (mg CaCO3/L)
(ml) ml ml ml
=90 to 110%)
T100 100 1Z 21.2 Iq.a 100 1 osolO Duplicate sam ple precision:
Sample Alkalinity
%RPD
=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3-L)
{(S - D)/[(S+D)/21} x 100 number (ml) ml ml ml I Multipe (acceptable range =: 10%e)
OS. tL.-
_AL-'JU-110 21.2
- 3.
- 11. 0 l
O
'.o S
jZ.
Duplicate (B)
I.
J-D eIO o,.I°1 Matrix spike recoveR:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (rng CaCO3/L)
(lng CaCO3/L)
(ml) ml ml ml 100 3,1.
NOWO Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (mg CaCO3/L)
= 75 to 125%)
1I10 5
100010 Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC0 3 /L)
O's -i's -q)
SArLTS, uJ to
()
t>
11.15 11.15 1(o, ko 12.0
_S__I___10 SS(AZ3 11.5 14.L?
3.1 14.U 6-Uo.0 oi.l\\.2o 0_.5 3.3,,.5 5.p2 02.
CC,-.%A*"*,
44,1 59 l~toz..
os-oo,-,o N '4
- 1)
,Z*
o.5.O.i*co*(2 osa 10 1_
J.Q45,X" 5%
I I
I i
I Reviewed by:
Date reviewed: b,9. I bO Page 64 of 99 SOP C6 - Exhibit C6.1, revision 09-01-09
0 Page 1.3 0F7 Page -_.._
Of Time initiated Time completed Alkalinity (SM 2320 B)
Matrix: Water, RL =1.0 mg CaCO 3/L Analyst I t
7?7 Date analyzed
.10-I "I]
Titrate* saples to 4I I
I iI Tftrant normality and multiplier determinatin N-pH of Normality Nmality(N) of 12504 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml Na 2CO3 x 0.05)/E
= (N x 50000)/100 ml sample water reference standard ml ml ml
= 0.25/E
=Nx500
= 4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Laboratorp control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (ing CaCO3/L)
(acceptable range (ing CaCO3/L)
(ml) ml ml ml
= 90 to 110%)
100 100 5*5.
-4 C.
1,,
t.101 0 Duplicate sam le precision:
Smple Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mng CaCO3/L)
{(S - D) /[(S+D)/2]} x 100 number(acceptable range_(ml) 10%)
Duplicate(B) 21.2.
d 9I 1 J-D 3,2010 Matrix spike recover:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(ma CaCO3/L)
(ml) ml ml ml Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (ma CaCO3/L)
=75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (ing CaCO31L os.11-ID M_______
100 OP%.O
ý 4
- %*oo Lao0 o I-,. M-10 1* Q,0 (D.4
- o/
Sot i.N, CIOO
,t.
- 0.
1_0_
Cb)____
11ý 1 11.4 4.R 90 OSII.g:L.
~
c~
I0O A1 ki t
Jo.-4 4 h0.
1 I
I I
I I
Reviewed by:
I kiIIII1 Date reviewed: [ 015. 1. L Q Page 65 of 99 SOP C6 - Exhibit C6.1, revision 09-01-09
0 0
9ETS Envimnmontal Testing Solutlont Page ___
Page
. __of Analyst [
J Date analyzed Titrant normality and msultintlpr pdc.,*.a, Alkalinifty (SM 2320 B)
Matrix: Wa.ter, RI Lb ii.gaC.
L Titiito sampqles to pH4/5,S.U Time initiated
,II Time completed I
I I.='
I pH of Normality N.r...abty (N) of HI*
pH Factor or Multiplier Deionized Titrant check Begin End Total
=( -
l x
O 3 zO**0I5)E
((Nx 50000)/100 ml sample water reference standard ml ml ml
= Nx 500
= 4.5 S.U.
number number (E)
(acceptable range 0.0180 - 06.0220)
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS =MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO3-L)
(ml) ml ml m
=90 to 110%)
-a,*ts 100 100 b.. B
- 14.
0lo Duplicate mple precision:
Sample Alkalinity
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
(aScetDbl/[(S+D)/2]) x 100 number (ml) ml ml ml I (acceptablerange=110%)
Duplicate (B)
I 41.,o
- 43 31.1 D
1-30 b0 o Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)
- (mg CaCO3 L)
(ml )
ml ml ml 00 2-15 4im 14,.5 to.
I 330 Sample alkalinity (B)
Measured spike value (MV)
% R =MV / SV x 100 (mg CaCO31L)
MV = A - B (acceptable range (mg CaCO3/L)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) botisls. ao FP.-M,,
+eQjzs 0.0 a1 3,4 Z&*_
4__
_0 I~tOll.O____
0oS1.ok-NN)&
00 01
- 3. 10(4 e-4 r.o jobs
- 10.
105 U-1__
________~1.
4__
LOS43A~te I_(______i.Cr I__
Q9.4 I~~
A
. L,,
Reviewed by:
F OLZ I
Date reviewed: I O. %.to Page 66 of 99 SOP C6 - Exhibit C6.1, revision 09-01-09
Page M _
Page
.4 Of S
koninmemalTeutfng Solution,. Inc.
Analyst Date analyzed E
~
Titrant mnn 71iltt, and mu~ailu
- ~
.,~..
1.r. dgo--
a-n,,r A-i pH of Normality
.61riality (N) of H 2S0 4 pH Factoi Multiplier Deionized Titrant check Begin End Total
= (5 ml Na2COs x 0.05)/E
= (Ni 5x001')I' 1 00 ml sample water reference standard ml ml ml
= 0.25/E L. N 500
= 4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RSMV/TV x 100i number (TV) volume Begin End Total Multiplier (ing CaCO3/L)
(acceptable range (Ing CaCO3/L)
(ml) mi ml ml
=90 to 110%)
I'Xv,1-4"kp 100 100 L
.L P5.3 q.5 lO.v 1
1 i/o 0
Duplicate sam le precision:
Sample Alkalinity S
RPD o Sample Sample ID I volume Begin End Total Multiplier (nag CaCO3IL)
((S - D) /r(S+D)/211 x 100 number I_
to%
(ml) ml ml mlI (acceptablerange=61 0%)
t______.____F_______________=* 2)__o
- .___3=__________________________
L00451-1.0i r44aTLTb% %W 15,3 2).o 5.- j 0-SA
-e 0
Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)
.(ma CaCO3AL)
(ml) ml ml ml 1-oo 2--i1 3
- 10. 5 IO.C 1)
Sample alkalinity (B)
Measured spike value (MV)
% Ron MV / SV x 100 (Ing CaCO3/L)
MV = A-B (acceptable range (mng CaCO3/L)
= 75 to 125%)
(oO9 it* CIo Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (mag CaCO3 /L)
IoO (I.. O__
IL tjw~&ftlo 1
5~-9
_________Z 1oos..*o, T
- A
~
0,.0 "A*
5",
u_114 I 086N. to IV 1
1 Joe Reviewed by:
Datereviewed:
Page 67 of 99 SOP C6 - Exhibit C6. I, revision 09-01-09
Page I t Page 5
of S Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L Analyst fI7II Date analyzed Ceý. wlo I
Time initiated I Time completed 1[-4 Titrate samples to pH = 4.5 S.U.
Tifrant nnrtrnilhtv nnd multinlip, dph',mlnatinv
_San no
-lt an
__e dtrmntin pH of Normality Normality (N) of H 2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml Na 2CO 3 x 0.0S)/E (N x 50000)/ 100 ml sample water reference standard ml mil ml
= 0.25/E
= Nx 500
= 4.5 S.U.
number number (E)
(acceptable range = 0.0180 - 0.0220)
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (rag CaCO3/L)
(ml) ml ml ml
= 90 to 110%)
100
'oo 34.-:"
44.3J q.S 10.4 IouD40ol Duplicate san, pie precision:"
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
((S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range =:L 10%)
TVAIKi
/oCSi2. 11-JEAu tLVMdt 10 0
-I4, 451 iOA.,
S Duplicate (B) 7 5
D Matri spik recoery:
pie__ alkalinity
_____A)
Reference standard
' Spike value Sample End number (SV) volume Begin End Total Multiplier (mg CaCO3IL)
(nmi CaCOP3 L)
(ml) ml ml ml 57ýg'+S f-o 10 00
- ).0 6.*
55
_e6 Il'k2 Sample alkalinity (B)
Measured spike value (MV)
% R MV i SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (mg CaCO3IL) 75 to 125%)
Sample measurements:
Sample volume Begin End Total' Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (mg CaCO3/L) 0OOSI 2.* 14 u,
Ao~argeA-e IOO 55l.
o.ko I* b I 00sa K U4 r.eAfl 1o.__
I I 1
.1 I 1.5-1 POOSI_._
1,2, 1
/pOS 7.. tat
_____________I 13J~.
So IF4 -
30
_____________phV?,C lL,.4 A j 5.4
(~00 Reviewed by:
EI iNZZA Date reviewed: 105.tt1,o I
Page 68 of 99 SOP C6 - Exhibit C6.1, revision 09-01-09
'0:
7 3
Gnv onmenta To
~n c.~lt no
. Page.
Page
\\
ofJ Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst FSI I1 Date analyzed t.
0
.t-Time initiated [
IC) IIIZ Time completed I
1111 Titrant normality and multinlier determination:
[i
[
ii Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E (Nx 50000)/50 ml sample number number (E)
(acceptable range = 0.0180 - 0.0220)
=N x 1000 ro,511-6
.1 *65 IVo 2.
.C
- 00.
- 0. 02-C),4 2z).4/
Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total.
Multiplier (Ing CaCO3/L)
(acceptable range (mg CaCO3iL)
(mI)
Ml MI Ml 90 to 110%)
5,,, 4 40 io j-1 1.9q 20.4 39 Oj, Duplicate sam le precision:
Sample Hardness
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
((S -D)/[(S+D)/2]} x I00 number (ml) ml mIl mil.
o.U.).-IO I-.
1
- 1.0 2-2-.o4 s
A3.
4 Duplicate(B)
I'4o le,..
2D2.
L -
1-o 45 4
-10 Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3/L)
(mi) mi l
ml M
559q50 14.0 118-
'2-2-0~4 Sample hardness (B)
Measured spike value (MV)
% R f MV / SV x 100 (mng CaCO3/L)
MV = A - B (acceptable range (mg CaCOs/L) 75 to 125%)
I5 o
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (mI) ml ml ml Multiplier (mg CaCO3/L)
TV =ND Blank (should be= 0 mg CaCO3/L)
.7
-0.,
OS.
14-.q 0*.O'lO-J
._._ 2*.
,-i5*
- q osi-1-_o 2
- 9.
-.1 4.
+
13 3I-Ii
[I II L
I
,Note: lf.5rIO itrant is used, sample must be diluted.
Reviewed by: I rageo o"W T
Date reviewed ICS-io to--
-1 SOP C7 - Exhibit C7.1, revision 09-01-09
o SEnvironmnsftal Testing eoulns n.
Page 6A Page ______of___
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst P,&
- 1 Date analyzed )5lt 1-I Time initiated Time completed Titrant normalitv and multiplier determination:
I 5
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml mi
= 0.2/E
= (N x 50000)/50 ml sample number number IE)
(acceptable range = 0.0180 - 0.0220)
= N x 1000 AJ I
Laboratory control standard:
Reference standard True value Sample Hardness (MV)
%RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO31L)
(ml) ml ml ml 90 to 110%)
- P4 15 1
- ,40 50
- .I3 LI 1* 2o4 3q 9j,0/,
Duplicate sam le precision:
Sample Hardness
%RPD =
.Sample Sample ID volume Begin End Total Multiplier (rag CaCO3/L)
{(S - D)/[(S+D)/2J} x 100 number (ml) ml ml ml 6S.,4-1o Z*
j,4 (A')
SO0 15.2-1l9.
4.-5 Z.-04 s
Z, 4
Duplicate (B)
It
)z.
LI, D
.2.1o R
CtD II I
I I
I
.(~amnIp mpa~urpmpnte' I
I Sample volume Begin End Total Hardness Sample-number Sample ID (ml) ml ml ml Multiplier (mu CaCO3/L)
TV = ND Blank (should be = 0 mg CaCO3/L)
.oo
- j. o NAl6 wAViAtv, Gi)
-so0 4,.0o 2q,o 8.0 2_o,4.__,_
,2i.A-0q.o 312 Z
55
/00S 14.O 6q21 CR-
___5 1oCjr:0.o4
.AL 1
0
- 44.34
-q L5)
IcqO
____i_
C) 3-,3 i
A+
1
?f-bO Lf1 Cu)tflb SO 4
- 53.
4D. I ljls I3 11 n~ O'
.vC-Fx g
o JD 12 434 o
5O0 I~
-2.
-4.
/
ý 70IfT1AY1 itrant is used, sample must be diluted.
Reviewed by: I lw.-
Date reviewed 1
(.Q.10 SOP C7 - Exhibit C7.1, revision 09-01-09
D:7 Page..
of 5
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO 3/L A n alyst I p l
.77I Date analyzed 1 05-1kv-1 Time initiated Time completed Titrant normality and multiDlier determination:
I I
I Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E
= (N x 50000)/50 ml sample number number (E)
(acceptable range - 0.0180 - 0.0220)
= Nx 1000 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
%RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (rag CaCO3/L)
(acceptable range (mg CaCO3/L)
(ml) ml ml ml
=90 to 110%)
40 50 1"+'
l"Z'.
2,1 2C)4 lO'ýO/o Duplicate sam le precision:
SampleHardness_
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (rag CaCO3/L)
((S - D) /[(S+D)/2]) x 100 number (ml) ml ml ml
-0 P 6Fto.1o ch cO C
o 60 I,.2 a.*
Il.(,
Zi.4 s
Duplicate (B)
Marix spike recoverv:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mag CaCO3IL)
(mg CaCO3/L)
(ml) ml ml ml
- -W1S
--tRI 40-
- 5 Iq-U
- 5.1 5.6 Z-.4 l11 Sample hardness (B)
Measured spike value (MV)
% R = MV / SV x 100 (Ing CaCO3/L)
MV = A - B (acceptable range (mg CaCo 3/L)
= 75 to 125%)
-:} 337 q2-010 Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV = ND Blank (should be = 0 mg CaCO3/L) aoostS.O1 Ca)
Ii a
iL
---h ax I~
10 0511 -CrL-T-JA16FNWI~
trA~eQ
__5 qL I 10 0S 15.0 TL,-
1 915 I
!A.o 3*1 3.- 15
/InSIS.0'L-
_______A
_~
42.-1o&15 1
)nor--,
_____1.__
Re____.3t 1)+o 3.3
/0 05 I-4. O' 06 0.0_
.3.
1 A+
100 o.* TL IVAIS 3_._
.1 Io o*q~5 A.o
.d...
m eb I
I I
I I
i I
i Note: If >15ml of titrant is used, sample must be diluted.
Reviewed by: F1*i*
iT1 Page 71 of 99 Date reviewed, 1
-k (0. to, I
SOP C7 - Exhibit C7.1, revision 09-01-09
I I
I I
I D
Envorenmental Testing Soluton, Inc.
Page 9o5 Page __
of S
Time initiated Time completed i i +/- ]
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst [12.77 Date analyzed [DIio I
I II Titrant normality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E (N x 50000)/50 ml sample nuumhbr nlumber (e
/AIerP net0hl8 r-n20 =
IV 0120*
fl 0 AI I
I-in I]
I' If Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier i (mg CaCO3iL)
(acceptable range (Mg CaCO3AL)
(ml) ml ml ml
=90 to 110%)
T-cNi3q 40 50
-+.0 1.1 IRA 2-o 4 4
0i°o Duplicate sam le precision:
Sample Hardness
%RPD
=
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
((S - D) /[(S+D)/21) x 100 number m
ml ml m-ypqsava tIm e
.1S Duplicate (B)
____.__________,________D________o__
Matrix spike recovery:.
Reference standard Spike value Sample Spike hardness'(A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(nmgCaCO3/L)
(MI) ml ml ml Sample hardness (B)
Measured spike value (MV)
% R MV / SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range (mi CaCO3/L)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (Tag CaCO3L)
TV = ND Blank (should be = 0 mg CaCO3/L) l,,-.i me1
- a.
.q 1,__1______
Io
, o-'a b+
452.o 3.15 1
ioos,2.I1 3)__
'p~L.10 36
- 4) a%'
/OCS 1 lZlL..
to2
%f~r 2__
TVAIK 29l-_
H pNagote: IL15rIlgtitrant is used, sample must be diluted.
rae/ oj 10yy Reviewed by: I ihL'-
7 Date reviewed 1 C6.16'10 SOP C7 -Exhibit C7.1, revision 09-01-09
Environmental Testing Solutions, Inc.
Page
- A Page S
of S
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst LýGIIIII- ]
Date analyzed L)r3.II,.IIII_
Time initiated [.I Time completed[
Titrant normality and multiplier determination:
1 l
i II Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
= 0.2/E
= (Nx 50000)!50 ml sample number number (E)
(acceptable range = 0.0180 -0.0220)
= Nx 1000 Laboratory control standard:
Reference standard True value Sample Hardness (MV)
% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (nag CaCO3/L)
(acceptable range
[1(rng CaCO3/L) ml ml 4 ml
=90 to 110%)
40 E50 44 Vi 1
.11 2-04 L;jCioj Duplicate sam le precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (Ing CaCOfL)
[(S -D) /[(S+D)/21) x 100 number (ml) ml ml ml
______.%5 Oawe~b Iso k
.df"..
g 2oD4 54 1u m b e r D u p lic a te (B )
I
- p.
A 1 Matrix spike recovery.:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3/L)
(ml) ml ml ml Sample hardness (B)
Measured spike value (MV)
% R MV / SV x 100 I(mg CaCO3 /L)
MV = A - B (acceptable range (rag CaCO3/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total_
Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
Blank TV=ND (should be = 0 mg CaC0QL
/0oSIX, i-..
1 tSO 5+"1+
)U3 l W 20.4 93 o1 S1 o
A.I q "VN(
L!*T~b v
-A----*---l
,6.
I6(o$as-0.
- 30. C54
+4 q
10 I6%jO
__/
i
-N 11 11
[1 P
Ilf731ý)dgq itrant is used, sample must be diluted.
Reviewed by: I lobO i Date reviewed 105 "!4.KJ SOP C7 - Exhibit C7.1, revision 09-01-09
Sequoyah Nuclear Plant Biomonitoring May 11 - 18, 2010 Appendix D Reference Toxicant Test and Control Chart Page 74 of 99
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Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.
1.2 1.0 0.8
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USEPA Control Limits (-+/- 2 Standard Deviations)
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I 0.6 0.4
~1.0 S
0.8 J0.6 0.4 I
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I USEEPtZaring d..Controt Limits_
th n r entile.CVs)
S.................................
1.2 1.0 0.8 0.6 0.4 I
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Laboratory Warning and Control Limits (10 and 25 Percentile CVs)
I.............
I ~ ~ ~ ~~~~
II I
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I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC 25)
Warning Limits (mean IC 25 +/- SA10 or SA.75) pa o r 9I imits (mean IC 25 SA.25, SA.90, or 2 Standard Deviations)
Pimtephales promtelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.
State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT S
Control Limits SAM Warning Limits SA-"
Control Limits SA.7s Warning Limits SAM Control Limits CV (g/L KCI)
(g/L KCI)
CT-2S CT+2S CT - SAJO C
+ SA,0 CT -SA s CT + Ss CT - SA.75 CT + SA.7s CT-SA.
CT +SA.9 1
06-18-09 0.78 2
07-07-09 0.72 0.75 0.04 0.66 0.83 0.09 0.66 0.84 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0.41 1.08 0.06 3
08-04-09 0.78 0.76 0.04 0.68 0.83 0.09 0.67 0.85 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.05 4
08-11-09 0.81 0.77 0.04 0.69 0.85 0.09 0.68 0.86 0.16 0.61 0.93 0.29 0.48 1.06 0.35 0.42 1.12 0.05 5
09-15-09 0.72 0.76 0.04 0.68 0.84.
0.09 0.67 0.85 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.05 6
10-06-09 0.78 0.76 0.04 0.69 0.84 0.09 0.67 0.86 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.11 0.05 7
10-14-09 0.77 0.76 0.03 0.70 0.83 0.09 0.67 0.86 0.16 0.60 0.93 0.29 0.47 1.06 0.34 0.42 1.11 0.04 8
10-28-09 0.73 0.76 0.03 0.69 0.83 0.09 0.67 0.85 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.05 9
11-03-09 0.67 0.75 0.04 0.66 0.84 0.09 0.66 0.84 0.16 0.59.
0.91 029 0.47 1.04 0.34 0.41 1.09 0.06 10 11-17-09 0.79 0.75 0:04 0.67 0.84 0.09 0.66 0.84 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.06 11 12-08-09 0.76 0.75 0.04 0.67 0.84 0.09 0.66 0.84 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 12 01-05-10 0.67 0.75 0.05 0.66 0.84 0.09 0.66 0.84 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0.41 1.08 0.06 13 01-12-10 0.77 0.75 0.04 0.66 0.84 0.09 0.66 0.84 0.16 0.59 0.91 0.28 0.46 1.03 0.34 0.41 1.09 0.06 14 02-02-10 0.66 0.74 0.05 0.65 0.84 0.09 0.65 0.83 0.16 0.59 0.90 0.28 0.46 1.03 0.33 0.41 1.08 0.07 15 02-11-10 0.70 0.74 0.05 0.64 0.84 0.09 0.65 0.83 0.16 0.58 0.90 0.28 0.46 1.02 0.33 0.41 1.07 0.07 16 03-09-10 0.70 0.74 0.05 0.64 0.83 0.09 0.65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.41 1.07 0.06 17 04-06-10 0.62 0.73 0.05 0.62 0.84 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 18 04-06-10 0.76 0.73 0.05 0.63 0.84 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 19 05-04-10 0.70 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 20 05-11-10 0.72 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 Note:
7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CF = Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the 1C25 values corresponding to the 10th and 25th percentile CVs.
These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
sO = Standard deviation corresponding to the 10 percentile CV. (SAis = 0.12)
)
S~ = Standard deviation corresponding to the 25" percentile CV. (SA2s = 0.21)
USEPA Control and Warning Limits SA,,7 = Standard deviation corresponding to the 75' percentile CV. ( SKT = 0.38)
SA" = Standard deviation corresponding to the 90& percentile CV. (SAws = 0.45)
CV = Coefficient of variation of the IC25 values.
USEPA. 2000. Undestlanding and Accountig for Method Vaiability at Whole Effiuent Toxicity Aplicatis Under the National Polhtant Disiharec Elinination Pngran. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, Oi Organisms obtained from Aquato, I=c.
pphrlcrl_051110
S TPrecision of Endpoint Measurements E T@
Pimephales promelas (Chronic Reference Toxicant Data EnvlronmentalTesting Solutions, Inc.
Test number I
Control Control Mean Survival Growth CT CV CT MSD PMSD CT for Control Growth
(%0)
(mg/larvae)
(mg/larvae) for Control.
(%)
Growth CV (%)
(%)
for PMSD (%)
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 06-18-09 07-07-09 08-04-09 08-11-09 09-15-09 10-06-09 10-14-09 10-28-09 11-03-09 11-17-09 12-08-09 01-05-10 01-12-10 02-02-10 02-11-10 03-09-10 04-06-10 04-06-10 05-04-10 05-11-10 100 100 100 100 100 100 97.5 100 100 97.5 97.5 97.5 100 100 100 100 100 100 97.5 100 0.790 0.763 0.692 0.583 0.723 0.894 0.758 0.855 0.757 0.825 0.917 0.918 0.769 1.019 0.831 0.846 1.101 1.010 0.871 0.901 0.776 0.748 0.707 0.710 0.741 0.743 0.757 0.757 0.764 0.778 0.789 0.788 0.804 0.806 0.809 0.826 0.836 0.838 0.841 6.7 5.7 6.2 8.0 9.6 12.9 7.6 6.5 7.1 8.3 10.0 9.0 4.0 17.7 5.3 5.2 4.9 7.9 9.7 1.8 6.2 6.2 6.7 7.2 8.2 8.1 7.9 7.8 7.9 8.1 8.1 7.8 8.5 8.3 8.1 7.9 7.9 8.0 7.7 0.09 0.11 0.07 0.05 0.08 0.12 0.14 0.10 0.07 0.10 0.13 0.11 0.08 0.17 0.07 0.07 0.10 0.14 0.13 0.07 11.7 14.2 10.7 9.3 10.4 13.2 18.3 12.0 8.8 11.8 14.6 12.1 10.7 16.5 8.5 8.4 8.9 14.3 15.0 7.7 13.0 12.2 11.5 11.3 11.6 12.5 12.5 12.1 12.0 12.3 12.3 12.1 12.5 12.2 12.0 11.8 11.9 12.1 11.9 Note:
CV = Coefficient of variation for control growth.
Lower CV bound determined by USEPA (10th percentile) = 3.5%.
Upper CV bound determined by USEPA (901 percentile) = 20%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10m percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
CT = Central Tendancy (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
Organisms obtainedfrom Aquatox, Inc.
Page 77 of 99 ppkclcr.05 1110
E Environmental Testing Solutions, Inc.
Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.
1.25 T" 1.00
'~
0.75 oim
,~..
0.50 0.25 30 20
- o
- 10 o
0 30 I I I
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USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
I I
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Kentucky Acceptance Limit (< 30.0%)
k-I I
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I USEPA Upper PMSD Bound (90t' percentile < 30.0%)
ri~
20 10 n
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- J Test date
Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD)
.......... Ppagq~tsykAimits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
3
.o*Env ronmenta lTesting Socutlns, ncc Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephales promelas PpKCICR Test Number:
17 I
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I Dilution preparation information:
Comments:
KCI Stock INSS number:
m*
- ll Stock preparation:
50 g KCI/L:
Dissolve 50 g KCI in l-L Milli-Q water.
Dilution prep (rg/L) 450 600 750 1
900 1050 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:
Test information:
Organism age:
0A d'
Moja. OL, Randomizing template:
Date and times organisms 1,
6O "0
t Incubator number and were born between:
shelf location:
Organism source:
Pe-0c S
o 0,- 1 C Artemia CHM number:
CAHS I k Drying information for weight determ in ation :
Transfer vessel pH =
S.U. Temperature =
°C Date / Time in oven:
6Si1%.10 its information:
"1. &
I Initial oven temperature:
t60, L.
Average transfer volume:
Date / Time out of oven:
O$.le-.o iSp
- 0.
Final oven temperature: j 0
Total drying time:
1+
- AWo".
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 0
OS,oK -o o
Il ls osd
-1 0
,t-*
SO'll it*O lOIS~
4*
O$.6'VIO 1
0$1-1 o-150 Ak ts io 6 10 -I-to) 2 s.,.o 0o iL*,
- AOit l
0S..io-Q 5
O---O "O
a- 06 1 ll 05-1.
7 1
o Control information:
Acceptance criteria Summarj of test endpoints:
% Mortality:
- 97.
<20%
7-day LC5 6
L.4A Average weight per initial larvae:
I-q0 NOEC
< (ASO Average weight per surviving larvae:
0.IO
> 0.25 mg/larvae LOEC Aso_
i I
I ChV IC25 Page 79 of 99 SOP AT21 - Exhibit AT21.1, revision 04-01-09
F00
~ Ervlro~rnten mJ~estlnt
- Soutions, Inc, Page 2 of 5 Species: Pimephales promelas PpKC1CR Test Number:
_7A Survival and Growth Data Day Control 450 mg KCIIL 600 mg KCIIL A
B C
D E
F G
H I
J K
L D
t l
ot
-oIot c
-1 0
/1
/0
/0 t0
(
0 (1
t0 10 (3
10 1(
(0
/ o (o
/0
/Q ItO
/0 (0
/0 1(0 J6 (0
(0
-2
/0
/0 10
/0
/0
/0
/0
/0 to
/0 to
/0 3
1_
/
/0
)0
'0
/0 to.
to
/0 19A
/0
/0 q'A 4
/0 o
0 10 1
10 1
0 to to
.O (0
A 5
to 1O 10 t.o to to 1o to 10 a>
6 10 10 10 o0
/0 10 10 4
10 I0
/o
/o
/Q
/0 /0 to 10 9
10
/0 q A = Pan weight (mg)
Tray color code::
1 0 tz.bs 15.SO I t'4eM 13aB
- 434, a1 IZ.92.
(Zi' Jo IZ
-Va Analyst:LA Date:
b - IS-t B = Pan + Larvae weight (mg)
Analyst:
.2 ZI.'t 22.Sq 22-90 Z3511 21.03 Zt.8 221.#2 221.18 20.-.3
- 2*90 UM.O 21.15 Date: r6-2-i-7 9'I C = Larvae weight (mg)
=B-A
,0 qo o
1*
,b I
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Weight per initial number of larvae (mg)
= C / Initial number of larvae 0!, I" I
w b,
W" 1".
V U
0 V
Average Percent weight per reduction Initial from control 0.,I o number of
(%)
larvae (mI 0.* 5 U.
7 O & O Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Page 80 of 99 SOP AT21 - Exhibit AT21.1, revision 04-01-09
- Envionment alTestn Solutani, Inc.
Page 3 of 5 Species: Pimephales promelas PpKCICR Test Number: "LO4 I
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Survival and Growth Data Day 750 m KCIIL 900 mg KCVL 1050 Mg KCI/L M
N 0
P Q
R S
T U
V W
X to
/
10 -o tO l to*
16 lo I tC) 10 /0 10 to 1
/ 1(
9A (0
,A 'A t&
1'I IV]
-9
-i
-41 2
to to a
-1 b
5 7
IVA ii W
IL& et. A U t-.
A Pan +Lra weight (mg)
Tray co code::
1s.0 1Not 17.940 0I(0 8l-W
- 1. 1.9q 17,'
It.23 l 7
S Analyst: LIAO--
I Date:, 5&ls
.- Ijo]"
C = Larvae weight (mg)I
=B-A
,,,A -"t.0 Sb (5,A 3 -0\\
-WI-B.
6-Weight per initial number
= Initinal number otftarvae y**
o
("
("
U Average o Percent 1."
,:k.
weight per reduction Initial Ifrom control 0 4.45 7-a-3-7, SA-1 0, 01/
C2l q.., S.
number of I(%
larvae
(
mg)
Comrnent codes: c = clear, d = dead, fg =fungus, k =killed, m =missing, skA sick, sm= unusually small, lg =unusually large, d&r = decanted and returned, w = wounded."alutinCnddareewd Analyst: ~
~
~
~
~
~
~
~
Clcltin and~ data? reviewed:.4 l7~I(8 l.~
Ii:2~
o3 40 I
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Comments:
Page 81 of 99 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09
1 Environmental Testing Solutions, Inc..
Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
PpKCICR 0204 Test dates:
May 11-18, 2010 Reveiwed by:
Comentration (mg/L Rtitheat litin number of Fbialnsmbero A Pan weight B=Pun+Lsrvae Larvat~glht(in)
Weight/Surniviag Meaz wuelghil Canlidenaldviao Weight/laitialumuber Mlesansvival M*enaeightlnitial C.ldeucl' Perncsn red-diofinin Kci) la"ae' buavae (nig) weighta)
=(A-8 numbe-oflaiv-se(me)
Survivingnumberoff ela'vue(mg)
(%)
namberolaivae variation (.)
contro (%)
larvae (1*)
(IN (mn)
A 10 10 12.85 2195 910 0.910 0.910 Control B
10 10 13.50 2259 9.09 0.909 0901 1.8 0.909 C
10 10 14.14 22.90 8.76 0.876 0.876 100.0 0.901 1i Not applicable D
t0 10 14.84 23.91 9.07 0.907 1
0.907 E
10 10 13.18 22.03 8.85 0.885 0.885 F
10 10 13.49 21.86 8.37 0.837 0.837 100.0 omt SA 7.8 G
10 10 14.39 22.62 8.23 0.823 0.823 H
10 10 13.41 21.18 7.77 0.777 0.777 1
10 9
12.92 20.23 7.31 0.812 0.731 600 J
10 10 14.40 22.90 8.50 0.850 0863 5.1 0.850 95.0 0.2 7.5 9.0 K
10 1 0 12.70 21.40 8.70 0.870 0.870 L
10 9
12.88 21.15 8.27 0.919 0.827 M
10 8
13.30 19.39 6.09 0.761 0.609 750' N
10 8
13.86 21.06 7.20 0.900 0.836 11.9 0.720 77.5 0.645 28.3 0
10 8
12.96 18.89 5.93 0.741 0.593 P
10 7
14.61 21.20 6.59 0.941 0.659 Q
10 4
13.60 17.79 4.19 1.048 0.419 R 90 R
3 13.88 16.89 3.01 1.003 0.952 13.0 0.301 42.5 0.00 19.9 55.6 S
10 5
12.96 17.89 4.93 0.986 0.493 T
10 5
14.07 17.92 3.85 0.770 1
0.385 1
U 10 2
14.50 16.23 1.73 0.865 0.173 1050 V
10 1
13.02 14.07 105 1.050 0.958 13.7 0.105 7.5 0.070 122.2 92.3 W
10 0
0.00 0.00 0.00 0.000 0.000 X
10 0
0.00 0.00 0.00 0.000 0.000 Dumnett's ISD value:
0.0691 MSD -
Minimum Significant Difference PMSD.
7.7 PMSD =
Percent Minimum Significant Difference PMSD is a measuxe of test precision. The PMSD is the mtinimnm percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%.
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the I 0th and 90th percentile, respectively, of PMSD data from EPA's WET Intedaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati OH.
S EnvimrnmentaI TestngSout IonsIc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:
5111/2010 Test ID:
PpKCICR Sample ID:
REF-RefToxicant End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potassium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Cone-mg/L.
1 2
3 4
D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.9000 1.0000 1.0000 0.9000 750 0.8000 0.8000 0.8000 0.7000 900 0.4000 0.3000 0.5000 0.5000 1050 0.2000 0.1000 0.0000 0.0000 Transformn Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV%
N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 1000 0
40 600 0.9500 0.9500 1.3305 1.2490 1.4120 7.072 4
14.00 10.00 2
40
- 750 0.7750 0.7750 1.0782 0.9912 1.1071 5.379 4
10.00 10.00 9
40
- 900 0.4250 0.4250 0.7088 0.5796 0.7854 13.871 4
10.00 10.00 23 40
'1050 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4
10.00 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93525082 0.884 0.18640681 0.44284928 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05)
NOEC LOEC CIV TU Steel's Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 12.7296549 1.68011061 9.43663803 16.0226718 0
2.07591082 7.81472778 0.56 2.92806711 0.07855673 3
Intercept
-32.273284 4.91966684
-41.915831
-22.630737 TSCR Point Probits mg/L 95% Fiducial Limits ECOI 2.674 556.309966 476.653416 611.406975 EC05 3.355 629.292849 561.145281 676.408122 EC1O 3.718 672.036124 611.495981 714.601419 EC15 3.964 702.503191 647.501036 742.147769 EC20 4.158 727.699548 677.169156 765.308341 EC25 4.326 750.034785 703.238413 786.272122 EC40 4.747 809.403342 770.273022 845.168712 EC50 5.000 847.358348 810.368169 886.267232 EC60 5.253 887.093169 849.541425 932.655984 EC75 5.674 957.310488 912.874788 1021.89777 EC80 5.842 986.693169 937.802481 1061.32683 EC85 6.036 1022.08245 966.999312 1110.03617 EC90 6.282 1068.41898 1004.2'1162 1175.46955 EC95 6.645 1140.98889 1060.85213 1281.01769 EC99 7.326 1290.67652 1173.5712 1508.17468 Organisms obtainedfrom Aquatox, Inc.
ppkclcr_0511I10
S FoTS 1ý Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/11/2010 Test ID:
PpKCICR Sample ID:
REF-Ref Toxicant End Date:
5/18/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
KCL-Potassium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
Conc-mg/L 1
2 3
4 D-Control 0.9100 0.9090 0.8760 0.9070 450 0.8850 0.8370 0.8230 0.7770 600 0.7310 0.8500 0.8700 0.8270 750 0.6090 0.7200 0.5930 0.6590 900 0.4190 0.3010 0.4930 0.3850 1050 0.1730 0.1050 0.0000 0.0000 Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min.
Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.9005 1.0000 0.9005 0.8760 0.9100 1.819 4
0.9005 1.0000
- 450 0.8305 0.9223 0.8305 0.7770 0.8850 5.354 4
2.207 2.180 0.0691 0.8305 0.9223
- 600 0.8195 0.9100 0.8195 0.7310 0.8700 7.512 4
2.554 2.180 0.0691 0.8195 0.9100 750 0.6453 0.7165 0.6453 0.5930 0.7200 8.867 4
0.6453 0.7165 900 0.3995 0.4436 0.3995 0.3010 0.4930 19.939 4
0.3995 0.4436 1050 0.0695
.0.0772 0.0695 0.0000 0.1730 122.184 4
0.0695 0.0772 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Willks Test indicates normal distribution (p > 0.01) 0.91475314 0.805
-0.882100479 0.97553825 Bartlett's Test indicates equal variances (p = 0.16) 3.65429521 9.2103405 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test
<450 450 0.06913843 0.07677782 0.007721333 0.00201167 0.062317923 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL(Exp)
Skew IC05*
289.45 88.49 137.15 662.35 0.9065 IC10 607.79 66.70 256.95 643.51
-1.3729 IC15 646.55 19.61 560.74 683.97
-1.2036 IC20 685.31 17.46 627;50 737.07 0.0018 IC25 724.07 18.45 664.67 781.51 0.2206 1C40 814.06 11.98 776.92 851.88 0.2789 IC50 869.02 16.35 822.81 925.35 0.2723
- indicates IC estimate less than the lowest concentration Organisms obtainedfrom Aquatox, Inc.
ppkcler_051 110
0 O
n
,* Svlrnmenal~stln Soutions, Inc.
Page 4 of 5 Species: Pimephales promelas PpKCICR Test Number:
UA Dailv Chemistry:
I Day 01 2
_____Analyst cS LCSb Concentration Parameter pH (S.U.)
.S"7
-7.
o
.7.'f gL.7.7
-7.8
- 7.
,:z.7..
Conductivity3 73S CONTROL Alkalinity (mg CaCO3IL) 61 Hardness (mg CaCO3/L)
Temperature 4
(0c)
- s
- ~
L.
PH L(S.U.) I 7 II
- -S-Z. I 450mgKCI/L Conductivity
,I 6+/-rnhoslcm)
Temperature I "-
pn(S.U.)
"7.Sq
.o 7c.a 0Te 600 mg KCI/L uctivity 1
(pmhos/cm)
Temperature IN1A I
I I
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I 750 mg KCI/L pH (S.U.)
I7.L (,,OI
-7 2-77,.*&2t DO (mg
-7.
-7.7 7
Conductivity IWOo ILID I Vo (rnihos/cm),
Temperature"
- 1..t
- 14. t pH (S.U77 79W-O Conductivity
,o I
(iimhos/cm)
Temperature
-LS.O
--L 3I~so pHs.u.)I-
-7.1g5 I
II
-7.1 g DO(mc/L) 7 A-I P9.
I
.W I
900 mg KCI/L I
1050 mg KCI/L Conductivity Temperature I-IA&
~
L I
-- I STOCK Conductivity I
I (gmnhos/cm)
I Initial I
SO AT2 -ExhibitJ 11L,"reisihn 0 9
SOP AT2I - Exhibit AT2I. 1, revision 04-01-09 Page 85 of 99
Envirown tb aTas ng$olut s Inc.
Page 5 of 5 Species: Pimephales promelas PpKCICR Test Number: "LOIA I
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I p
I r
~Day' Concentration Parameter I ~PH (S.U.)
I "a
I*
159c 7*q Conductivity 1
(ptmhos/cm)
CONTROL Alkalinity (mg CaCO3/L)
Hardness (rag CaCO3/L)
'*q, lX Temperature rA PH (S.U.)
- o.
1.*8 "4a 7.k07 "74.
"75 450 mg KCI/L Conductivity m
g (itmhos/cm)
Temperature
_o_____.'
"J,4.
1q.10
-*1~
n~,
-_,_. 7.'*
-*.s.
PH (S.U.)
- ).t.oo
-&-to 1.01JA 3%,
"7*1 DO (mg/L) 0,9-
-V 600 mg KCIL Conductivity 12 41 IW O
(*)2hoslcm) 1310*
Temperature 114.1 P H (S.U.)
f,l z*
1.' 1 ' --
A.
,Lo U"4,1 4/-/
-7
- 7
.7.3 DO(gL 1.8 V. 1
~
71 750 mg KCI/L Conductivity Temperature
. 1 "Li (0c)
PH (S.U.)
3
.1
-o 1I-h-4 7
7(5
_z_
DO9
"/.l, 7._
"4"-
.1 67.7 t "
900 mg KCI/L Conductivity I
(i[tmhos/cm)
Temperature "q..
-14 1
.Lk.
A pH (S.U.)
,41
.+5
- .4 7.5 i(',,
DO (mg/L) 19 t "0 A 787 1050 mg KCIL Conductivity 30A Z.OO muhos/cm)
Al
.3 Temperature v..
I".
2.q.i
- t. 0.
- "*4, 43 iq,1 "6.3
-(----
I I
Initial Final Initial Final Initial Final IInitial FinalI SOP AT21 - Exhibit AT2 1.1, revision.04-01-09 Page 86 of 99
/2
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Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1.14 1.12 1.10 1.08 1.06 1.04 1.02 USEPA Control Limits (+2 Standard Deviations)
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2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 I
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USEPA Warning and Control Limits (75 th and 9 0 th Percentile CVs)
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I Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 0.........so......
0......
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o.......s*.............0................................
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I 1.0 0.9 0.8 Test date 7-day IC2. = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC 25 +/- SA10 or SA75)
......... Control Limits (mean IC25 + SA25, S A90, or 2 Standard Deviations)
P~g2 S7 of99
I Environmental Testing Solutions, Inc.
Test number Test date 7-day IC25 CT (g/L NaCI)
(g/L NaCI)
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 03-10-09 04-14-09 05-05-09 05-05-09 06-09-09 06-09-09 06-18-09 07-07-09 08-04-09 08-04-09 09-15-09 10-06-09 10-14-09 11-10-09 12-08-09 01-05-10 02-02-10 03-02-10 04-06-10 05-04-10 1.07 1.09 1.07 1.08 1.07 1.07 1.06 1.06 1.07 1.09 1.05 1.08 1.07 1.07 1.05 1.08 1.07 1.08 1.05 1.09 1.08 1.08 1.08 1.08 1.08 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 1.07 State and USEPA S
Control Limits SAJo CT-2S CT+2S 0.02 1.05 1.12 0.09 0.01 1.05 1.11 0.09 0.01 1.05 1.10 0.09 0.01 1.06 1.10 0.09 0.01 1.06.
1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.10 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.09 0.09 0.01 1.05 1.10 0.09 Laboratory Warning Limits CT - SA.10 CT + SA.I0 Laboratory SAM Control Limits CT - SA.2S CT + SA.ZS USEPA SA.T 7
Warning Limits CT - SCTS CT + SA.75 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 0.99 USEPA SA.90 Control Limits CT - SAm CT + SAm 1.17 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.90 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 0.89 1.26 1.26 1.26 1.26 1.26 1.26 1.26 1.25 1.26 1.25 1.26 1.26 1.26 1.25 1.25 1.25 1.26 1.25 1.26 0.49 0.59 0A8 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0.48 0.59 0A8 0.59 1.57 1.56 1.56 1.56 1.56 1.56 1.56 1.55 1.56 1.55 1.56 1.56 1.56 1.55 1.55 1.55 1.56 1.55 1.56 0.67 0.67 0.67 0.67 0.67 0.67 0.67 0.66 0.67 0.66 0.67 0.67 0.67 0.66 0.66 0.66 0.67 0.66 0.67 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 0.41 1.75 1.74 1.75 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 1.74 CV 0.02 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Note:
7-d IC, = 7-day 25% inhibition concentration. An estimation ofthe concentration of sodium chloride that would cause a 25% reduction in Cerzodaphnia reproduction for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values..
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint S,_jo = Standard deviation corresponding to the 10 percentile CV. (Skis = 0.08)
SAM = Standard deviation corresponding to the 2 5 h percentile CV. (SA.25 = 0.17)
USEPA Control and Warning Limits SA7s - Standard deviation corresponding to the 75'h percentile CV. (Sk.5 = 0.45)
SA.90= Standard deviation corresponding to the 90C percentile CV. (Skwgo
= 0.62)
CV = Coefficient of variation of the IC25 values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OL
Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Environmental Testing Solutions, Inc.
Test number Control Control Mean Survival Reproduction
(%)
(offspring/female) 1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 03-10-09 04-14-09 05-05-09 05-05-09 06-09-09 06-09-09 06-18-09 07-07-09 08-04-09 08-04-09 09-15-09 10-06-09 10-14-09 11-10-09 12-08-09 01-05-10 02-02-10 03-02-10 04-06-10 05-04-10 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 i00 100 31.9 33.9 33.6 34.6 31.3 31.1 32.3 29.9 30.4 32.0 31.6 31.4 33.3 34.0 35.2 31.1 31.9 31.0 32.7 31.0 CT for Control Mean Reproduction (offspring/female) 32.9 33.1 33.5 33.1 32.7 32.7 32.3 32.1 32.1 32.1 32.0 32.1 32.2 32.4 32.4 32.3 32.3 32.3 32.2 CV CT for Control
(%)
Reproduction CV (%)
MSD P'MSD CT
(%)
for PMSD (%)
5.8 6.7 4.3 7.7 6.4 9.4 7.3 3.3 4.7 5.1 5.4 4.3 3.5 6.2 4.6 5.8 3.5 6.3 6.3 4.6 6.3 5.6 6.1 6.2 6.7 6.8 6.4 6.2 6.1 6.0 5.9 5.7 5.7 5.7 5.7 5.5 5.6 5.6 5.6 2.6 2.0 2.3 2.2 2.1 2.6 1.9 2.2 2.2 2.2 2.2 2.4 2.6 2.6 2.0 2.3 2.6 2.4 2.4 2.3 8.2 5.8 6.8 6.5 6.7 8.3 5.8 7.2 7.3 6.9 7.0 7.6 7.7 7.7 5.8 7.3 8.3 7.7 7.4 7.4 7.0 6.9 6.8 6.8 7.0 6.9 6.9 7.0 7.0 7.0 7.0 7.1 7.1 7.0 7.0 7.1 7.1 7.2 7.2 Note:
CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (10th percentile) = 8.9%.
Upper CV bound determined by USEPA (90th percentile) = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90t" percentile) = 47%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
CdNaCICR_050410 Page 89 of 99
SEnvironmental Testing SolUtions, Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 0
0*
0 35 30
- 25 o
20 15 I
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I Minimum Acceptance Criteria (> 15.0, offspring per surviving female)
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I North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
40 30
.~Co 20 50 40 30 20
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I USEPA Upper PMSD Bound (90* percentile < 47.0%)
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%/
Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD) tr imits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) rage *-qkoAV0,
77 Page I of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCICR #:
p6 Dilution preparation information:
Comments:
NaCl Stock INSS number:
l 900ý Stock preparation:
100 g NaCI/L:
Dissolve 50 g NaCI in 500 mL Milli-Q water.
Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491.
1488 1485 1482 1479 1
Total volume (mL) 1500 1500 1500 1500 1500 Test organism Source information:
Test information:
Organism age:
< 24-hours old Randomizing template color:
6ty%
Date and times organisms were born (3S,'0+10 0(.a6 TO 01 Incubator number and shelf between:
location:
Culture board:
I- &
-W
__-10 A
Replicate number:
1 2
3 4
1 5 1 6 1 7 1 8 9 110 YWT batch:
10 Culture board cup number:
Ss 1
1--
I IS I,, Z.
2.1l'thl-
_-1_
Transfer vessel information:
pH =*,*
S.U. Temperature = ".
O C
Selenastrum batch:
oI.-3~l JO Average transfer volume (mL):
- 0. - 0165_
_0 Daily renewal information:
Day Date Test initiation and feeding,-
MHSW Analyst renewal and feeding, or batch used termination time 0
os.CA.io AOc 04-m-.o0 lb xi 2
os.oS.-,o 0&11
,, o.o,.1/oA 3
'o01-10 Otto uS,.o'lof0 4
05- 0 f-10 DO k'i %
bs,.o.*0 Aif g
to-10O 0
1 os-t6-iO Contrbl information:
Acceptance criteria Summar, of test endpoints:
% of Male Adults:
< 20%
7-day LC50 2j I j.
% Adults having 3' Broods:
ItŽny.
80%
NOEC ROO
% Mortality:
< 20%
LOEC 1000 Mean Offspring/Female:
31.>
15.0 offspring/female ChV w A
%CV:
4.167.?
1
< 40.0 %
IC25 1041 -1, Page 91 of 99 SOP AT14 - Exhibit AT14.1, revision 04-01-09
EnT 10 Page 2 of 6 Species: Ceriodaphnia dubia CONTROL CdNaCICR #:
I 0(o Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7 1 8 9
10 I
Young produced C)
C*(
3
(
)
- n n
n
.. C
')
Adult mortality k
2 Y o u n g p ro d u ced
( ) 1c*
J.
0 0
Lc L
-t-A dult m orta lity t--
U....-...
2
ýYoung produced 0~
0 QL 0
L Adult mortality t1 1
t_
t_
4_ Yon prdue Lk I Adult mortality
]
5 Young produced j
j_
7z
.1 I\\
11 c I'.
Adult mortality I
\\-I-I-
6 Young produced Q
0 0
0 IAdult mortality t1'J--
-I
- I 7
Y oung produced I
C), t\\
\\i ]J A is i*2.
T o ta l y o u n g p r o d u c e d a
0 C )
0 0
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5C) al a?-
7A 1
.5 U Final Adult Mortality U.
k.1--
L..-.
\\--.-
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X for 3' Broods
=
)<.
I C-5 Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:I
% Mortality:
1 07.
Mean OffsprinmgFemale:
31-0 600 mg NaC/L Survival and Reproduction Data Replicate number Day 1
1 2 3
4 5
6 7
8 9
10 1
Young produced 1
0 I
Adult mortality L
J U L-.
J,_
U U-U.__
2
°ong produced j'J,
° o
Q',
I Adult mortality L
I-
=
L-..
3 Young produced a
n
.0 Adult mortality [
, U-.....
t-I I
'-**I %-
4 Young produced 131 LA S
S
[X 9 s
r SIYugrded '_Adult mortality t-l-*_
%_°*
\\._' '
- 5 You"n g r oduced
%lb t~~o O l O 0 1 Adult mortality [_-...
j j_.
U U.
U U
5 Young produced 0
IL V.
I1[10 11 0 C) o~
Autmortality L
" 7 Young produced lLI =I I\\-
11, O ]25 I S Total young produced 4
52-l
- h.
81 a
3
- \\
Final Adult Mortality
-=.
L U
It C I Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
I Concentration:
% Mortality:
7.
Mean Offsnrine/Female:
I APIF
% Reduction from Control:
-t:9"/
SOP AT14-Exhibit AT14.1, revision 04-01-09 Page 92 of 99
77F Page 3 of 6 Species: Ceriodaghnia dubia 800 mg NaCI/L CdNaCICR #:
I-O 6 Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
I Young produced 0
0 00 C
C)
Adult mortality t.'
L L
L L
L 2 Young producedJ~ 01 C>
C aJ 00]
Adl otlt
- I
=-
(-=-
L 3
Young produced C) 0 Adult mortality._
J I O
O 1
4i Youngproduced Adult mortality V
5 Young____
prdce rL V
'0:tII'
~-I-I z-Adult mortality L
\\
6 jYoung produced C1(Ž 0 r0 1
Q 0
0 Adult mortality k]
I "-
L-U 7]
Young produced 1
%"1 I
k"1.
i q I1M 11 Total young produced ff
-b2 S
3 a%
.5
- 3.
3 S
30 b
,.1 Final Adult Mortality L_
L_......
C 1
L.
I.
Note: Adult mortality (L = live, D = dead), SB-split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer).
- _Concentration___
IConcentration:I
~~%
Mortality:
OS Mean Offspring/Female:
,3k. A
% Reduction from Control:
- I 1000 mg NaCI/L Survival and Reproduction Data Replicate number Day.
1 1 2 3
4 5
6 7
8 9
10 I
Young produced
)
O C.
3, I)
C I Adult mortality L-t--
2 Young produced I
1 C) 0 a
0 Adult mortality L-I L-I V-I=
A
=Lk 3
Young produced Cdtmrty
- l CC 0
0ljO Adult mortality
_t-.
Lj L_.._
[ Young produced
'4
-I-
-t-5 Young produced 10 tL jo o T 'o Ii
[-L-I L.
Adult mortality Sum L
jI L
I.
i Y
Young produced
=) J C) I I
C\\
1_
01 7
Young produced IS 19 o
=
23 IA r= T-Total young produced zi I Z:5I, 'a -1 1 j
11 2.-1. 1 --1i 1 2. 1 Z
I%0 1 I \\- 1 -\\- I %- I ZZ I \\.- 1 --C I %.-- 1 %-- I %.- I %-Z Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortaliy:
07.
Mean Offspring/Female:
2,11
% Reduction from Control:
".M'7, SOP AT14 - Exhibit AT 14.1, revision 04-01-09 Page 93 of 99
Species: Ceriodaphnia dubia I
1200 mg NaCI/L Page 4 of 6 CdNaCICR #:
6 0 o Survival and Reproduction Data B
Replicate number Day 1 112 3
4 5
6 7
8 9
10 1
- Young produced C
)
Adult mortality I_.
k-L.
2 Young produced 0..~..~
O T
.o C > C 01 Adult mortality L.
3 Young produced 1 0TC 0
0 0
Adult mortality k.....
L.
4 Young produced
[ Adult mortality F
.° 5
Young produced S
I 1
4 T 17 S
j
[Adult mnortality'
~
~-
L C.
6 Young produced 0:
10 0
0 Q
7 Young produced 14 7o1 1 '4 i
Total young produced 0
I I I
t -
i'
- z..
- 2.
. I" Final Adult Mortality L_
t I.--
i--
C C
C Note: Adult mortality (L = live, D = dead), SB =split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality:
Mean Offspring/Female:
I"Ii.
0
% Reduction from Control:
A'Ab,'7, 1400 mg NaCI/L Survival and Reproduction Data Replicate number Da.
1 2
3 4
5 6
7 8
9 110 1
Young produced
.L 1
0 O
0 Adult mortality L....
L J '..
2 Young produced C) 0 A
l mortality
=
I
=1
=
=-
3 Young produced 0
0 0
0 0 1 Adult mortality 3
Young produced 0j)
Q 0~.
o o 0
1 0) T Adult mortality ]
. Lt..
5 Young produced C) 0
- )
C)
_ Q [
, I
-1 0 Adult mortality L]EC I
\\-
I t --
-II L4L LJ-6 Young produced ]070 0 1 I C 0 C J
___Adult mortality 17L.
7 Young produced Li TT Total young produced 1 1 l 1
'4 "1
{.
Final Adult Mortality C.
-=
\\..._
W t W.
Note: Adult mortality (L live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult durina transfer).
I Concentration:
% Mortality:
(7.
Mean Offspring/Female:
1
,40 I % Reduction from Control: I S4.'7I SOP AT 14 - Exhibit AT 14. 1, revision 04-01-09 Page 94 of 99
1.RI SEnvironmental Testing Solutions, Inc.
Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
Test dates:
Reveiwed by:
CdNaCICR #106 May 04-11, 2010 I-Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variation (%)
control (%)
Control 32 32 30 30 33 29 32 29 31 32 100 31.0 4.6 Not applicable 600 34 32 31 32 31 30 31 31 31 33 100 31.6 3.7
-1.9 800 32 32 31 31 30 32 35 30 30 31 100 31.4 4.8
-1.3 1000 29 33 27 29 28 27 27 29 28 30 100 28.7 6.4 7.4 1200 20 17 12 17 17 19 26 21 12 15 100 17.6 24.0 43.2 1400 7
4 1
4 4
6 5
5 7
6 100 4.9 36.6 84.2 Dunnett's MSD value:
PMSD:
2.288 7.4 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 13%.
Upper PMSD bound determined by USEPA (90th percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001 b).
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
S S
Statistical Analyses
- 0 Environmental Testing Solutions, Inc-Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/4/2010 Test ID:
CdNaCICR Sample ID:
REF-Ref Toxicant End Date:
5/11/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
NACL-Sodium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10 D-Control 32.000 32.000 30.000 30.000 33.000 29.000 32.000 29.000 31.000 32.000 600 34,000 32.000 31.000 32.000.
31.000 30.000 31.000 31.000 31.000 33.000 800 32,000 32.000 31.000 31.000 30.000 32.000 35.000 30.000 30.000 31.000 1000 29.000 33.000 27.000 29.000 28.000 27.000,
27.000 29.000 28.000 30.000 1200 20.000 17.000 12.000 17.000 17.000 19.000 26.000 21.000 12.000 15.000 1400 7.000 4.000 1.000 4.000 4.000 6.000 5.000 5.000 7.000 6.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Miin Max CV%
N Stun Critical Mean N-Mean D-Control 31.000 1.0000 31.000 29.000 33.000 4.562 10 31.333 1.0000 600 31.600 1.0194 31.600 30.000 34.000 3.715 10 114.00 75.00 31.333 1.0000 800 31.400 1.0129 31.400 30.000 35.000 4.795 10 108.50 75.00 31.333 1.0000
- 1000 28.700 0.9258 28.700 27.000 33.000 6.372 10 70.50 75.00 28.700 0.9160
- 1200 17.600 0.5677 17.600 12.000 26.000 23.987 10 55.00 75.00 17.600 0.5617
- 1400 4.900 0.1581 4.900 1.000 7.000 36.571 10 55.00 75.00 4.900 0.1564 Auxiliary Tests Statistic Critical Skew Kurt Kohnogorov D Test indicates normal distribution (p > 0.01) 0.890369 1.035 0.62537854 4.07545315 Bartlett's Test indicates unequal variances (p = 4,77E-04) 22.2131653 15.0862722 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU Steel's Many-One Rank Test 800 1000 894.427191 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL Skew IC05 ICI 0 IC15 IC20 IC25 IC40 IC50 918.987342 31.6875687 861.4625 985.564423 0.5821 1009.00901 16.6380586 963.25 1025.13816
-0.9637 1037.23724 10.3476841 1016.68642 1055.4655 0.3765 1065.46547 11.473116 1044.96121 1089.83315 0.9599 1093.69369 13.6658949 1072.20238 1125.38548 1.3582 1178.37838 18.9439593 1145.54695 1217.24402 0.4973 1230.44619 16.3902417 1192.18208 1260.74352
-0.1532 CdNaCICR_050410
'C
'C 0
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/4/2010 Test ID:
CdNaC1CR Sample ID:
REF-Ref Toxicant End Date:
5/11/2010 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
NACL-Sodium chloride Sample Date:
Protocol:
FWCHR-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comments:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10 D-Control 32.000 32.000 30.000 30.000 33.000 29.000 32.000 29.000 31.000 32.000 600 34.000 32.000 31.000 32.000 31.000 30.000 31.000 31.000 31.000 33.000 800 32.000 32.000 31.000 31.000 30.000 32.000 35.000 30.000 30.000 31.000 1000 29.000 33.000 27.000 29.000 28.000 27.000 27.000 29.000 28.000 30.000 1200 20.000 17.000 12.000 17.000 17.000 19.000 26.000 21.000 12.000 15.000 1400 7.000.
4.000 1.000 4.000 4.000 6.000 5.000 5.000 7.000 6.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 31.000 1.0000 31.000 29.000 33;000 4.562 10 600 31.600 1.0194 31.600 30.000 34.000 3.715 10
-0.600 2.287 2.288 800 31.400 1.0129 31.400 30.000 35.000 4.795 10
-0.400 2.287 2.288
- 1000 28.700 0.9258 28.700 27.000 33.000 6.372 10 2.299 2.287 2.288
- 1200 17.600 0.5677 17.600 12.000 26.000 23.987 10 13.395 2.287 2.288
'1400 4.900 0.1581 4.900 1.000 7.000 36.571 10 26.090 2.287 2.288 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.890369 1.035 0.62537854 4.07545315 Bartlett's Test indicates unequal variances (p = 4.77E-04) 22.2131653
. 15.0862722 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE.
F-Prob df Dunnett's Test 800 1000 894.427191 2.28751339 0.07379075 1178.28 5.0037037 2.3E-35 5,54 Treatments vs D-Conirol Analysis used for PMSD calculation only.
CdNaCICR_050410
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I Species: Ceriodaphnia dubia CdNaCICR #:
It0 Daily Chemistry:
I Day i
UI I
1 I
Analyst I Concentration= Parameter pH (S.U.)
DO (rag/L)
Conductivity (Amhos/cm)
CONTROL Alkalinity (mg CaCO3/L)
"1.5n Lo
./
4q ISo -_
zs Hardness (mg CaCO3/L)
Temperature (003 I
-PH (S.U.)
" '?
-~ Jl 2
600 mg NaCI/L Conductivity iqqo I '*/5W6 (g~mhos/cm)
Temperature IS L.,.-I PH (S.U.)
o
'ih 800 mg NaCIIL Conductivity
'1030 tO 0
(iimhos/cm)
Temperature V
-. 3 a-
"L
-1
¢(c)
Codutiitm.'.-
-j PH (S.U.)
"1.'11-.L2
-4'3
-,"/
Q.
DO (m/L)
-I. I.
1000 mg NaCI/L Conductivity W0 I
(pmnhos/cm)
Temperature S
, C)
DO (mg'O&
9
.z 1200 mg NaCI/L Conductivity SO25L (grnhos/cm)
Temperature (0c S.-
I I
1 I
I I
I pH (S.U.)
DO (ma/U) 1400 mg NaCI/L Conductivity (gmuhos/cmn) 11i[4 Z.
Temperature (0C)
STOCK Conductivity I (grmhos/cm)
I/-
Initial F
SOP AT14 - Exhibit AT14.1, revision 04-01-09 Page 98 of 99
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Species: Ceriodaphnia dubia CdNaCICR#: M
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Day
.3 4
5 6
Concentration Parameter DO (mg/L) 718.6
~
Conductivity
([tmhos/cm)
- 32.
23, CONTROL Alkalinity (mg CaCO3/L):5 Hardness 0
(Mg CaCO3/L)
Temperature W -to
__(0c)____
(0 R
pH (S.U.)
ca. rf I
-7.C
. '.q' DO (mg/L) 600 mg NaCI/L Conductivity (gimhos/cm) 150 P31 I3O I S Temperature I
- 2.
4 PH(S.U.)
71
- .0.9 7.1
-. 1 7,
- 7. 9 DO (mg/L) 6 0
800 mg NaCl/L.
Conductivity 80 (jAmhos/cm) 6 Temperature ILIA
" -a 6 (aC)
PH (S.U.)
4iu (0 1000 mg NaCI/L Conductivity V0
- /-/I (gmhos/cm)
APO01f Temperature
-LA t
- 0c_
M.__.
._,)
1200 mg NaCI/L Conductivity
([tmhos/cm) 20 Temperature j,,
2 14.
(00400 1400 mg NaCIJL Conductivity
~c9 (gmnhos/cm) 241 q1 Temperature 0
.?.
"L4.O
.S.O (0C)
Initial II Final I Initial 11 Final I Initial 11 Final I Initial II Final I
U -
U -
U -
I -
U U
-4 41614 Icc.
C.s&Ak.vr ~
fVo.
t~b11 a~ebwr~eb shm iA61G
-gAW~LM,-Af 5 Page 99 of 99 SOP AT14 - Exhibit AT14.1, revision 04-01-09