ML100150093
| ML100150093 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 01/11/2010 |
| From: | Church C Tennessee Valley Authority |
| To: | Cromer P Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML100150093 (117) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 January 11, 2010 State of Tennessee Department of Environment and Conservation
.Division of Water Pollution Control Enforcement & Compliance Section 61h Floor,. L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Patrick Cromer:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR DECEMBER 2009 Enclosed is the December 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.
Sincerely, Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): .
Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue.
Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C,. 20555
' " " " ' y.l LA' Printedon recycted paper
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) Form Approved.
MAJOR DISCHARGE MONITORING REPORT (DMR) i Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0 004 Address P.O. BOX 2000 (INTEROFFICE SB-2A-SNN) F - FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUM ER DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLEAR. PLANT Locatio HAMILTON COUNTY MFNIToRING PEEI"D EFFLUENT YEAS I MQ IA m_ LYuEA I mO A From 091I12101 1TO 09 1 12 1 31 *** NO. DISCHARGE IZ **
ATTN: Stephanie A. Howard NOTE: Read instructions before completiric thi*sform.
PARAMETER OUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE **13.9 0_o 31 /*31 MODELD MEASUREMENT 04 CENTIGRADE 00010' Z '0 0 PERMIT .30.5 DEG.C. SEE' CKREQ INSTREAM MONITORING REQUIREMENT PERMIT TEMPERATURE, WATER DEG. SAMPLE **28.2 0 0 31/*31 RCORDR*
CENTIGRADE MEASUREMENT 00010 1 0 0 PER IT "* *. ', O.-
REPORT DEG.C. .,.SEE .CK RE'Q EFFLUENT GROSS VALUE -REQUIR.EMENT.' , 'i'7'"'-" DAILY MX7 PERMIT........
TEMP. DIFF. BETWEEN SAMP. & SAMPLE 29.** 0 0
0 31 /31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 W 0 PERMIT .... 5.0 DEG. C... "' CONTIN' CALCTD EFFLUENT GROSS VALUE REQUIREMENT '," , DAL MX PH SAMPLE 7.5 7.8 12 0 5/31 GRAB MEASUREMENT 00400 1 0 0 PERMIT " SU WEEKLY 'GRAB EFFLUENT GROSS VALUE REQUIREMENT .. MINIMUM .' ' " MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ** 5 5 19 0 1./ 31 GRAB MEASUREMENT 00530 1 0 0 ' PERMIT. ','MOL30 3 ,' 100 ' 11IL ' . MONTHLY G EFFLUENT GROSS VALUE . . IREME.Tij " .MO AVG. DAILY MX' OIL AND GREASE SAMPLE <5 19 0 1 / 31 GRAB MEASUREMENT 00556 1 0 0 ."PERMI,'K**** .****, 2. 5 '2 MGIL <'MONTHLY GRAB EFFLUENT GROSS VALUE REQUIREMENT> "Ki'."h" :K.'<,: "' 'AiDAIMX FLOW, IN CONDUIT OR THRU SAMPLE 1599 03 . 1 31 / 31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 0 "PERMIT REPOR MGD . CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT 'M" R DAIL T' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were.prepared under my .TELEPHONE DATE
-irection or supervision in accordance with a system designed to ,assure that qualified personnel.
Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WaBN Sequyah Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, *_423 843-7001 10 01 07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED RER COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachmentshei No closed mode operation. The following inf6rmation is included in an attachment: 1. CCW Data 2. Veliger Monitoring Data EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons' Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.
CCW CHANNEL. _ _ _ _.,
Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/09/2009 @ 1010 <0.10 mg/I 12/11/2009 @ 0425 KMF EPH
NOTES:%
Water Mean# of Water SUB
- Mean# of Gravid Asiatic COLLECTED BY Sample Date % Settlers Sample Date Asiatic LOCATION ZMS3/ Temp.D tC) Temp. (*C), LOCATION lamsm Clam.......
11/03/2009 133 0 16 11/03/2009 76 16 Inplant RCW RCW CMW 11/10/2009 417 6.1 16 11/10/2009 25 16 Inplant CMW 11/17/2009 269 0 16 11/17/2009 0 16 Inplant RCW CMW 11/24/2009 36 50 15 11/24/2009 18 15. Inplant RCW CMW 12/01/2009 32 0. 13.5 12/01/2009 0 13.5 Inplant RCW WE 12/08/2009 38 0 11 12/08/2009 0 11 Inplant RCW CMW I
I
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT. DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-01004 Address P.O. BOX 2000 F - FINAL (INTEROFFICE SB-2A-S-N)
SODDY - DAISY, TN 37384 PERMIT NUMBER [_DSCHARG N DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLEAR PLANT EFFLUENT LoCatio HAMILTON COUNTY "
FomNITRING IyFAR? InMO AY I ýY MF AR Mn A
".. NO DISCHARGE
- From{ 09 12 { 1IT{0 12 I3 ATTN: Stephanie A. Howard NOTE: Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING ,QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
' EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL . SAMPLE MEASUREMENT
. 0.015 0.025 19
.1 00 12/31 11.41 GRAB GA 500.60 1 0 0 . PERMIT. 0.10 0.10 MG/L I WEEK- .CALCTD EFFLUENT GROSS VALUE E MI MO AVG INST MAX YS TEMPERATURE - C, RATE OF SAMPLE
- 0 62 **3 31 / 31 CALCTD CHANGE MEASUREMENT 82234 1 0 0 PERMIT... >!' 2' DEG , C iNTi CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CIHR : UUS SAMPLE MEASUREMENT PERMIT...' .. . . . . . . . . . .. . . . . . ...
REQUIREMENTP SAMPLE MEASUREMENT PERMIT.
REQUIREMENT,___
SAMPLE MEASUREMENT PERMIT..............
REQUIREMENT SAMPLE MEASUREMENT PERMIT; REQUIREMENtT SAMPLE MEASUREMENT I.PERMIT K REQUIRýEMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my
. direction or supervision in accordance with a system designed to assure that qualified personnel
- (> , TELEPHONE DATE Christopher R. Church, properly gather and evaluate the information submitted. Based on my inqdiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN information, the information submitted is, to the best of my knowledge and belief, true, _ _* _423 843-7001 10 01 07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER, YEAR MO DAY TYPED OR PRINTED ""o COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachmentsbet Page 2 of,2 EPA Form 3320-1 (REV Form 3320-1 3/99)
(REV 3/99) Previous editions may Previous editions may bebe used.
used Page 2 of. 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEOUOYAH NUCLEAR PLANT (SUBR 01) CMB No. 2040-0004 Address P.O. BOX 2000 -
(INTEROFFICE SB-2A-SON) TN0026450 0 F-FINAL SODDY - DAISY. TN 37384 PERMIT uUMBER NUMBER DIFFUSER DISCHARGE Facility TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY MONITDDITPING, PE:RIDQ EFFLUENT From o 09 1a 10 01 1 To1 ToAYYER 09 12 M 31
- DANODICHARGEY ODSHREL__
- ATTN: Stephanie A. Howard NOTE: Read instructions. before completinq this form.
PARAMETER QUANTITY OR LOADING -,OUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS , MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS BORON, TOTAL SAMPLE MEASUREMENT. .. .<0.20 **19 0 1/92 01022 1 0 0 .<.PERMIT **** ' REPORT MGIL I OTRLY" GRAB' EFFLUENT GROSS VALUE . REQUIREMENT i i .
SAMPLE MEASUREMENT PERMIT ,,
REQUIREMENT_,,
SAMPLE MEASUREMENT REASUIREMENT ,
SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT
'PERMIT
.ITEQU°REMENT'fl SAMPLE MEASUREMENT PERMIT REOUIREMENT SAMPLE MEASUREMENT
.REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER TCertify under penalty of law that this document and all attachments were prepared under my . - I TELEPHONE DATE 1direction or supervision in accordance with a system designed to assure that qualified personnel c -
Christopher R. Church property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN 423 843-7001 10 01 07 information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA R YEAR MO DAY TYPED OR PRINTED I OFFICERORAUTHORIEDAGENTAREA
_Y NUMBER__EAR__O__
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceallattaclrmentshet Boron was sampled on 10/07/2009 @ 1237.
EPA Form 3320-1 (REV 3199) '. Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT % (SUBR 01)
Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON) TN0026450 101 T F-FFINAL A SODDY - DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY M I nA'h l r*~ D I"
I*P r-F.I[SWU I EFFLUENT FrYoAR From 1 09 I12 mM Oil01TOY 0 DA' 1a 3
- NO DISCHARGE "
ATTN: Stephanie A. Howard NOTE: Read instructions before cornpletinq this form.
PARAMETER OUANTITY OR LOADING OUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE ** Monitoring 23 CERIODAPHNIA MEASUREMENTNot Not Reqitre Required in TRP3B 1 0 0 PERMiT .45.2 PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMEN MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMPLE Monitoring 23 PIMEPHALES HA E MEASUREMENT *IE Nontore Not Required ng 23
_OS TRP6C 1 ' 0 0 PERMIT 45.2 PERCENT SEE G*OMPS EFFLUENT GROSS VALUE REQUIREMENT> .. .A:, 4M5iM.IUMPER MIT SAMPLE MEASUREMENT PERMIT REQPUIREMENT
. *SAMPLE MEASUREMENT AERMIT REQUIREMENT.
SAMPLE MEASUREMENT PERMIT',
REQUIREMENTM SAMPLE MEASUREMENT PERMIT"
REQUIREMENT,.
SAMPLE MEASUREMENT PERMIT>
,REQUIREMENT~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this.document and all attachments were prepared under my [." E OD personnel direction or supervision in accordance with a system designed to assure that qualified Christopher R. Church . properly gather and evaluate the information submitted. Based on my inquiry of.the person or rsd n persons whothe information, manage the system, information or those submitted is, topersons the bestdirectly responsibleand of my knowledge for belief, gathering the true, Environmental Manager SON & WBN "423 843-7001 10 01 07 "euy hSt Vc Sequoyah Site Vice accurate, and complete. I am aware that there are signifi'cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED .
information, including the.possibility of fine and imprisonment for knowing violations.
____________________________ , OFFICER OR AUTHORIZED AGENT OFCRO AUHRIE AGNT AREA ARAN NUMBER UME YEAR ER MO M
DAY A
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachlmentshe; Toxicity was not sampled in December 2009. Report from November 2009 toxicity sampling is attached.
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of I
December 10,_ 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING,.NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, NOVEMBER, 2009 Per your request, I am only submitting an electronic copy of the subject report.
The report provides results of compliance testing, using fathead minnows and daphnids. Outfall 101, samples collected November 15-20, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Sherrard, R. M., PSC 1X-C Files, OE&R, CEB IB-M SQN November 2009M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: December 10, 2009
- 1. Facility / Discharger: Sequoyah Nuclear Plant TVA
- 2. County_ State: Hamilton / Tennessee
- 3. NPDES Permit #:. TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1,579
,6. Receiving Stream- TennesseeRiver (TRM 483.6)
- 7. lQI0: 3,491
- 8. Outfall Tested: 101
- 9. Dates Sampled: November 15-20, 2009
- 10. Average Flow on Days Sampled (MGD): 870, 873, 1761
- 11. Pertinent Site Conditions: Production / operation data will be provided upon request.
1.2. Test Dates: November 17-24, 2009
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead. Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72-.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2,72.6, 100 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%)" Outfall 101: IC 25 =45.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: IC2 5 > 100%
Ceriodaphniadubia:. IC2.5 > 100%/
UV treated Outfall 101: Pimephalespromelas: IC 25 > 100%
Page 1 of 102
- 18. Facility
Contact:
Ann Hurt Phone #: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364 21.TVA
Contact:
Cynthia L. Russell Phone #:(256) 386-2755
- 22. Notes: Outfall 101 samples collected November 15 - 20, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 1.00 percent. Exposure of minnows and daphnids to intake samples resulted
.in no significant difference from the controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah..
Page2 of 102
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. SampleType: Composite
-.- 3. Sample Information,:
Date . Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)
Sample Time (ET) Time (ET) Temp. t TRC* Time (ET)
ID Collected Received (°C) (mg/L) Last Used By 101 11-15-09 0944 to 11-17-09 1500 11-16-09 0844 11-17-09 1315 0.6,0.7 <0.10 11-18-09 1413 11-15-09 1014 to 111709 1315 1.1 11-17-09 1500 11-16-09 0914 11-18-09 1413 11-17-09 0745 to 1.3;' 1.4 <0.10 11-19-09 1400 11011118091505 11-18-09 0645 11180911-20-09, 1411 11-17-09 0805 to 11-19-09 1400 Intake 18-09 0705 11-18-09 1505 0.7 <0.10 11-20-09 1411 11-20-09 1405 101 11-19-09 0735 to 11-20-09 1440 0.8, 0.9 <0.1.0 11-21-09 1418 11-20-09 0635 11-22-09 1410 11-19-09 0756 to 11-20-09 1,405 Intake 11-20-09 1440 1.0 <0.10 11-21:09 1418 11-20-09 0656 11-22-09 1410
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 + 1.0°C) in a warm water bath.,
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
Page3,of102
Pimephalespromelas Ceriodaphniadubia
. Test Organisms:
- 1. Source: Aquatox, Inc. In-house Cultures
- 2. Age: 22.50-22.73 hours8.449074e-4 days <br />0.0203 hours <br />1.207011e-4 weeks <br />2.77765e-5 months <br /> old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic. Moderatelv Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate:. 10 1
- 6. Test Initiation: (Date/Time)
Outfall 101 11-17-09 1444 ET 11-17-09 1500 ET UV Treated Outfall 101 11-17-09 1430 ET
- 7. Test Termination: (Date/Time)
Outfall 101 11-24-09 1346 ET 11-24-09 1402 ET UV Treated Outfall 101 "11-24-09 1331 ET
.8. Test Temperature: Outfall 101: Mean = 24.8 0C Mean = 24.9°C (24.5 - 25.3 0 C) (24.6 - 25.3 0C)
Test Temperature: UV-Treated Outfall 101: Mean = 24.9 0 C (24.5 - 25.30 C) 9L Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in .one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for DH, and dissolved oxvgen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToXCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
Page4of102
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) *(Type / Duration)
Conducted November 17 - 24, 2009 using effluent from Outfall 101.
Test. Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100
.1.1.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 98 98 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 *100 98 90 88 88 Test Solutions Mean Dry Weight (mg) eSluent)s(re plicate number)
(% Effluent) 1 2 3 4 Mean Control 0.830 0.817 0.842 0.797 0.822 11.3% 0.857 0.820 0.819 0.839 0.834 22.6% 0.849 0.792 0.794 0.884 0.830 45.2% 0.782 0.832 0.849 0.772 0.809 72.6% 0.806 0.758 0.770 0.781 0.779 100.0% 0.852 0.792 0.697 0.716 0.764 Intake 0.397 0.778 0.801 0.889 0.716 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA, Lower Limit: NAA
- TUa 100/LC 50: TUc = 100/IC2 5 Page 5 of 102
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a C-eriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted November 17 - 24, 2009 using effluent from Outfall 101.
Percent Surviving Test (time interval used.-: days)
Solutions 1 2 33
(% Effluent) 1 .2 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100" 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100
-72.6% 100 100 100 100 100 100 100 100.0% 100 .100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)
Data (replicate number)
(%Effluent) 1]2 13 4 5 6 7 8 9 10 Mean
.Control 32- 29 26 30 27 30 28 29 31 33 29.5 11.3% 32- 31 28 31 30 30 30 28 29 33 30.2 22.6% 32 32 29 32 32 32 32 32 32 34 31.9 45.2% 33 34 33 31 32 36 30 34 33 32 32.8 72.6% 33 34 34 32 37 33 38 34 30 32 33.7 100.0% 35 36 32 36 34 36 38 36 38 38 35.9 IC25 Value:.> 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50 : TUc = 100/IC 25 Page 6 of 102
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted November 17 - 24, 2009 using water from Intake Percent Surviving Test (time interval, used - days)
SolutionsrT (oluens 1 2
(%Effluent), -3 4. 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)
%Effluent). Data (replicate number) ent 1 2 3 4 5 6 7 8 9 10 Mean Control 31 27 30 32 28 30 31 30 28 29.8 Intake 38 35 33 30 29 33 32 3133 32.5 IC2 5 Value: > 100%. Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa*= 100/LC5 o: TUc = 100/IC2 5 Page 7 of 102
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted November 17 - 24, 2009 using effluent from UV Treated Outfall 101.,
Test Percent Surviving "Solutions. 1 2 (time interval used - days)
(% Effluent)- 1 2 3 4 5 6 7 Control i00 100 100 100 100 100 100 11.3% 100 100 100 100
- 100 98 98 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100l 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(%Effluent) 2 (replicate number)
(%_Effluent)_ 1 2 3 ". 4 [ Mean Control 0.848 0.718 0.772 0.870 0.802 11.3% 0.876 0.857 0.764 0.755 0.813 22.6% 0.749. 0.796 0.854 0.806 0.801 45.2% 0.724 0.816 0.825 0.865 0.808 72.6% 0.890 0.775 0.856 0.814 0.834 100.0% 0.775 0.757 0.939 0.867 .-0.835 Intake 0.813 0.726 0.780 0.770 0.772 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA!
Lower Limit: NA
- TUa = 100/LC 50 : TUc = 100/IC2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) -
Species Date
- Time Duration Toxicant Results (IC2 5)
Pimephalespromelas November 17-24, 2009 1220 17-days KCI
- 0.79 g/L Ceriodaphniadubia November 10-17, 2009 1115 6-days NaC1 1.07 g/L I
I - NaCI Page 8 of 102
PHYSICAL/CHEMICAL
SUMMARY
..Water ......... . *. Mean Chemnistry ea Values and ]Ranges for Pimephalesprornelas and Ceriodaphnia .a .a1fo dubia Tests, Non-treated Sequoah ......
Y......
Nuclear Plant (SQN) Outfall .,.... 101 - . ...........
performed November 17-24, 2009.
Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial . Final Initial Final Initial Final (fLmhos/cm) (mg/L CaCO 3 ) (mg/L CaCO 3 ) Chlorine (mg/L) 24.8 24.7 7.8 7.4 7.58 7.37 332 62 90 Control 24.7 - 24.8 24.6 -24.8 7.6 - 8.0 6.9 -7.7 7.42 7.79 7.20 7.56 325 - 336 61 62 88 . .90 .
11.3 % f~24.8'2 1 24.7 7.....7.9 7.3 7.66 .7.32 308
... - i ............
247 250 24.5 - 249 7.7 8.2 6.9 - 7.6 . 7.57 7.79 '75.6 750 297 - 317 -. '-
22.6% .....................................
24.9 24.8 8.0 7.1 7.63 7.29 291 - -
- ...:7...............
24.7 -:25.0 24.6 25.0 7.8 - 8.2 6.3 7.6 7.53.7.74 7.11 - 7.50 280 299 -. -
24.9 24.8 8.0 7.1 7.61 7.29 256 45.2%
24.7 25.1 24.6 - 25.0 7.7 - 8.2 6.4 - 7.7 7.47 7.73 7.14 - 7.51 249 - 266 - .
7 2 .6 % 24.9 -........24.7 8.1 7.2 ........... 7.57 . 7.28 .....218 . ..
._ _ 24.7 - 25.1 24.5 24.9 7.7. - 8.3 6.6 7.8 7.41 7.70 7.04 - 7.51 211 - 225 - - -
25.0 24.7 8.1 7.2 7752 7,25 172 75 87 " <0.10
__100.0 24:7 - 25.3 24.5 - 25.1 7.7 - 8.3 6.5 - *7.7 7.32 7.67 7.01- 7.46 166 - 181 62 -. 96 i69' 110 <0.10 <0.10 24.9 24.7 8.2 7.2 7.49 7.24 170 63 .75 . <.0.10 Intake 24.8 - 25.0 24.6 -. 25.1 7.8 8..4 6.5 7.9 7.26 f. 7.64 '7.0I - 7.49 .163 - 179 58 - 66 69 - 82 <0.10 < 0.10 C ontro l 24.8
. -- 25.0 ...... ................
7.8 . 7.9 7.58 . 7.59.. . . . 332
. . . . . 62. . . . . .90 24.6 -24.9 24.9 -253 7.6 - 8.0 7.7 - 8.1 7.42 - 7.79 7.51 -7.71 325 - 336 61 - 62 88 - 90' -
1% 24.8 25.0 7.9 7.9 7.66 7.59 309 -
11"3%___ 24.7 - 24.9 24.9 - 25.2 -7.7 - 8.2 7.6 - 8.1 7.57 -. 7.79 7.52 -.* 71 297 - 317 . .... . . .... .
"6% 24.9 25.0 8.0 - 7.9 7.63 7.59 291 "
"2 % 24.7 -25.0 25.0 25.1 7.8 - 8.2 7.5 8w1 7.53 -7.74 7.52- 7.71 -280 - 299 - - - - - . -
44.%
5 .2 % 24:9 .. . .25.0.
...... ....... 8.0 7.9 7.61 .... .7.58
, ..... ..... 256 .............. . ... .... ......
24.7 25.0 24.9 - 258.2 7 7.6 8.2 7.47 -. 7.73 7.51 - 7.71 249 266 . .. . - - -
24.9 24.9 8.1 7.9 7.57
............. 7.57 218 ....
7 2.6%........-...
24.8- 250 24.8- 25.1 7.7 - 83 7.6 - 8.2 7.41 ..7.70 7.48- 7.69 211 - 225 - . - . . _
25.0 25.0 8.1
- 7.9 7.52 7.55 172 75 87 < 0.10 24.8 - 25.2 24.8 - 25.2 7.7 - 8.3 7.6 - 8.2 7.32 _ 7.67 7.46 - 7.66 166 - 181 62 - 96 69 - 110 <o.1i0 a<01O 25.0 25.0 8.2 7.9 7.49 7.56 170 63 75 < 0.10 24.9 25.0 24.8 25.2 7.8 - 8.4 7.6 - 8.1 7.26 -7.64 7.46 - 7.66 163 - 79 58 - 66 69 - 82 <0.10 - <0.10 i..............
i-.*. ... ..~ .. .. ... ....i.i............
i.... ..........
i .............
............ ..i............
..i Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.8 24.5 25.3 Ceriodaphnia dubia 24.9 24.6 25.3 Page 9 of 102
PRV5IUCAT /CIW.MIrAT STIMU ARV Water Chemnistry Mean Values and Ranges for Pimephalesprom elas Tests, UJV- treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed ....November
......i.....17-24, 2009..... .............
..........................- * = ::
Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity
. . . . . ............ :1 Hardness .... ....
Initial Final Initial Final Initial Final (tmhos/cm) (mg/L CaCO 3 ) (mg/L CaCO 3 )
Control 24.8 24.8 8.0 7.3 7.61 7.35 321 61 89 24.7 - 25.0 24.7 24.9 7.9 - 8.2 6.4 - 8.1 7.53 - 7.68 7.19 - 7.53 312 - 332 61 - 62 86 - 90 24.9 12 4.8 7.9 7.3 7.62 7.33 308 24.8 - 25.1 24.6 - 25.0 7.7 - 8.0 6.9 7.6 7.54 - 7.68 7.16 .-7.51 301 - 312 . .. : . .. . ....
24.9 24.7 7.9 7.3 7.61 7.33 293 24.8 - 25.1 24.6 - 24.9 7.8 8.1 7.1 - 7.7 7.53 - 7.69 7.16 - 7.52 290 - 299 ...... ......
25.0 24.8 8.0 7.3 7.59 7.31 265 45.2%
24.8 - 25.2 24.7 - 24.8 7.9 - 8.1 7.0 -, 7.7 7.49 - 7.68 7.15 - 7.49 259 - 273 , ... _......_
726% 25.0 24.8 8.0 7.2.- 7.55 7.30 218 24.8 - 25.2 24.6 - 25.0 7.8 - 8.1 6.8 - 7.7 7.433 - 7.65 7.11 7.48 214 223 2 . . . .... ._...
100.0% 25.1 24.8 8.0 7.3 7.51 7.28 173 63 74 24.9 .-. 25.3 24.7 .-. 24.9 8.0 - 8.2 6.9 -, 7.8 7.37 - 7.62 7.09 - 7.47 169 - 181 58--68 65 -~ 78 7.51 171 89. .. 63 66 72 78.........
_Intake 24.9 25.0
- 25.2 24."24.5-2.5 24.7 252 24.8 .4.88.0 8.0 8.1.-. 8.3' 8.3 6.9 6.9 7.3 7.8
- 7. 7.37.
7.37 - 7.6 7.617.77.077.26 7.47 .4. 160 16 18 60 60 6 69 69 i8 Overall
........ temperatureC(C) . . ... .pr... Average Minimum Maximum
......... :...:.........A
!S.....
! .e..... s.....e .as 24.9
- 24.5 25.3 Page 10 of 102
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected November 15-20, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were
>.100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this' study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples..
Page 11 of 102
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA-82 1-R-02-013. (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia.
Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS /MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None 2". Ceriodaphniadubia None Page 12 of 102
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance -logbooks.
3: Temperature was measured by SM 2550 B.
- 5. The pH was measured by SM 4500 H+ B.
- 6. Conductance was measured by SM .2510 B.
- 7. Alkalinity was measured by SM 2320 B.
- 8. Total hardness was measured by SM 2340 C.
- 9. Total residual chlorine was measured by ORION Electrode Method 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this-report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in glL KC1 or NaCl.
Sodium Chloride (NaC1 crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
Page 13 of 102
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Standard Methods for the Examination of Water and Wastewater, 2ls1 Edition, 2005.
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
Page 14 of 102
Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used, to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test .Sampling Page 15 of .102
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom PCL-401 CL-363 Cuprostat- H-13OMT:
Hypochlorlte mg/L mg/L nig/L PF ~mg/L~
mg/L TRC Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 _
03/13/1998 0,05:i -
03/14/1998 0.013 -
03/15/1998 0.030 .
03/16/1998 0,013 -
03/17/1998 0.020 P 03/18/1998 i 0,018 -
09/08/1998 0.015 - 0.005 ..- 0,021 09/09/1998 0.003 - 0.011 ---
09/10/1998 ) 70.014 - -- 0.021 --
09/11/1998 0.0xo13l3 0.019 -
0:015 09/12/1998 * <*. .0 1 0.015 09/13/1998 <00001*
09/14/1998 0..008 0.015 A: - - i 02/22/1999 <0.002 1 02/23/1999 0.005 -
02/24/1999 0009 02/25/1999
- 0.0 2. -
02/26/1999 0,008 "
02/27/1999 0.0011 02/28/1999 <0001I 08/18/1999 0.015 0.024 0.006 08/19/i999 0.012 0.024 -
08/20/1999 0.023 0.024 0.120 08/21/1999 0.022 0.024 08/22/1999 i 0.022 0.024 i!
08/23/1999 . . > 0.025 0.024 00O06' -
08/24/1999 0.016 0.023 QO.020 -
Page 16 of 102
Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom PCL-2M - PCL-401 CL-363 Cuprostat- -1130M Hypochlorite mg/L mg/L mg/L mgi/l PF mg. L
- mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000 <0.002 0.026 0.009 02/01/2000- 0.011 0.026 0.028 -
02/02/2000 0.028 0.026 0.009 0,006 02/03/2000 0.008 .0.027 0.009 02/04/2000 0.006 0i.027 0.009 0.005 0.109 020520< 0.002 oo 0.027 0.009 _
02/06/2000 < 0.002 *0,027 0.009 " . -
07/26/2000 <0.0057 0,055 0.019 - -
07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0d.-053 0.018 U04 . 0.108 07/29/2000 < 0.0088 0.(055 0.019 -
07/30/2000 <0.0076 0,055 0.019 - - -i 07/31/2000 < 0.0152 0 .055 0.019 0,006 08/01/2000 <0.0141 0:055 0.019 O0.005 12/11/2000 0.0143 0025 0020 0.005 12/12/2000 0.0092 0.025, 0.020 0.005 12/13/2000 <0.0120 0.025 0.020 12/14/2000 <0.0087 0.25 0.020 12/15/2000. 0.0120 0,025, 0.020 0.005 12/16/2000 < 0.0036 0.025 0.020 12/17/2000, <0.0036 0.025 0.020 08/26/2001 0.0017 0 :06 0.021 0.006 - -
08/27/200 1 .. <0.'0096 0.06. 0.021 0,005 0.0021 08/28/2001 <0.0085 0.06) 0.0021 .:
08/29/2001 ý<0.0094 U159 0.020 0.005 - 0.0-11 08/30/2001 <0.0123 0,.06 0.021 0.005 - -;
08/31/20011 <0.005 1,0.059 0 0.020 1 - I 11/25/2001 <0.0044 - -
11/26/2001 <0.00119 0.024 0.02 0.005 11/27/2001 0.0137 0.023 . 0.019 0.007 11/28/2001 <0.0089 0.022 0.019 0.006 11/29/2001........0.0132 0.024 0.02 0.007 11/30/2001 < 0.0043 '0 4 0.02 12/09/20011o <0.0042 7. - -
12/10/2001 <0.0042-12/11/2001 <0.0104 12/12/2001 0.0128 0.024 0.02 0,008 12/13/2001 <0.0088 0.024 0.02 -
12/14/2001 0.0134 0.024 0.02 0.007 Page 17 of 102
Table-B:L.Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to -Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date &6Sdifu*um Towerbrom PCL-:222.>, PCL-401 CL-363 Cuprostat- H-130M Hypochorite . mg/L mig/L mg/L Img/L PF mg/IL mg/I TRC Phosphate Coopolymfier DMAD mgIL Quat TRC Azole' 01/102/2002 < 0.0079 0.023 ' 0.02 0.006 01/03/2002 -< 0.0042 0.023.:K 0.014 -
01/04/2002 0.0124 0024: 0.014 0.009 -
0 1/05/2002 < 0.0042--
01/06/2002 < 0.0042 - - --
01/07/20021 <0.0089 1 0.024 0 0.014 0.006 ' 1 02/24/2002 < 0.004 - - -
02/25/2002 < 0.004 0.023 0,023 02/26/2002 0.0143 U23 0.03 0.023 0.007 -
02/27/2002 < 0.0041 0j!O.023 0.023 - ,,
02/28/2002 < 0.0041 0,024 " 0.008 03/01/2002 < 0.0041 1 0.024 0.008 - -
05/05/2002 C ~ K-' --
05/06/2002 -0.058 0.02 0.014 05/07/2002 0
'*"C02058 -0.02 0.01r5, 05/08/2002 0.056 U 00.19 -
05/09/2002 -0.057 0U2 0.014 05/10/2002 0.056 -1 0.019 08/04/2002 <0.0058 -- -
08/05/2002 <0.0058 09,.053 0.018 0.025 08/06/2002 0.0092 0.053 0.018 08/07/2002 .<0.0107 .0.055 0.019 0.007 08/08/2002 <0.0061 07&.055 0.019 -
0810912002 0.0152 0.054 0018 1 0.008 1 10/06/2002 <0.00497 >. - -
1010712002 . 0.0153 0.054-. 0.018 0.009 10/08/2002 <0.0092 0.018 0 00 10/09/2002 0.0124 0.053 0.018 0.007 10/10/2002 0.0134 j0.054 0.018 0.009 10/11/2002 <0.0042 1. 0.054 1 0.018 01/12/2003 .. .... <0.0035 - rKiJ 01/13/2003 <0.0069 0.025 0.019 0.009 0114/02003 <0.01318 0*.026 0.020 01/15/2003 <0.0063 0.026 0.020 0,009 0 1/16/2003 <0.0034 0.0126 0.020 01/17/2003 <0.0034 0.026 0.009 -
04/06/2003. <0.0073 0.021 04/07/2003 <0.018073 ~ .2 04/08/2003 <0.0 117 0.021- -
04/09/2003 <0 *0139 -0.02 ' 1 0,016 04/10/2003 < <0.0113 0.021 0.018S 04/11/2003 <0.0073 0.022 Page 18 of 102
Table.B-1.(continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom PCL-222 PCL-401 CL 363 Cuprostat-IHypochlorite mg/L S mgL* mg/L mg/L . PF ing/L TRC Phosphate Copolymer DMAD mg/L TRC Azole 06/15/2003< <*0.0045 '1 06/16/2003 < 0.0037 0.057 0.020 06/17/2003 < 0.0048 0.041 0.014 06/18/2003 < 0.0048 0,041 0.014 06/19/2003 <0.0085 0.058 0.020 06/20/20031 < 0.0048 <i0.058 0.020 08/03/2003 <0.0050 - - - -
08/04/2003 <0.0050 0.058 0.020 -
08/05/2003 <0.0051 0,057 0.020 08/06/2003 - <0.0084 0.0517 0.020-08/07/2003 0.0129 0.057 0.020 - .
08/08/2003 0.0153 0.057* 0.020 >0,00 10/05/2003 <00043
- 4. 0 ý01)057 0.020 < " -
10/06/2003 <0.0043 .0.057 0.020 . -* -
10/07/2003 - <0.0090 0 .057 .0.020 :
10/08/2003 <0.0106 0.7 0,5.7 0.020 -
10/09/2003 , 0.0181 0.0)26 0.022 10/10/2003 0.0183 0.026 1 0.024 0.009 02/01/2004 0.0093 0.027< 0.009 .
02/02/2004 <0.0034 0. 02*6 0.009 02/03/2004 <0.0034 026) 0.009 -
02/04/2004 . - 0.0124 0.062 0.009 0,009 02/05/2004 <0.0034 0.026 : 0.009 02/06/2004 0.0105 0.026 0.00 9 0.010 05/04/2-00-4 <0.0123 0.6-026. 0.019 -
05/05/2004. <0.0144 0.026< 0.014 0.009 05/06/2004 <0.01 46 0.037 0.013 05/07/2004 0.0227 0.058 0.020 0,009 05/08/2004 0.016. 0.060 0.021 - -
05/09/2004 <0.0104 0.058 0.020 07/04/2004 0.0217 0.057 , 0.01.9. , ,
07/05/2004 <0.0085 , 0.057 0.020 i0,009 -
07/06/2004 <0.0077 . 0.058 0.020...
07/07/2004 0.0252 .0.056. 0.019 07/08/2004 0.0223 0.0 57 0.019 0009 07/09/2004 0.0182 0.057w 0.020 0,009 -
.V Page 19 of 102
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 L November 20, 2009 Date: Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF. HI-130M Nalco Hypochiorit mg/L mg/iL mg/L mg/L mg/L mgi. 73551 e TRC Phosphate Copolymer DMAD Azole Quat mg/L mg/i.L EG/PO 11/07/2004 <0.0187 " 0 0.014 11/08/2004 <0.0192 0.047 0.030 >- - - -
11/09/2004 <0.0233 0,048 . 0.016 0.0O41 11/10/2004 <0.0149 0047 0.016 0,041 -
11/11/2004 <0.0149 0.049 0017 0.043 -
11/12/2004 <0.0253 0.048 0.017 0.042 -
02/06/2005 <0.0042 0.028 0.010 -
02/07/2005 <0.0 116 0.028 0.010 . 0.007 02/08/2005 <0.0080 : 0.028 0.010 -,
02/09/2005 0.0199 0.028 0.010 - -
02/10/2005 <0,0042 0.028 0.0 10--
02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 - - ,~* -
06/06/2005 0.0043 06/07/2005 0.0103 -
06/08/2005 0.0295 06/09/2005 0.0129 -
06/10/2005 0.0184 - -
07/17/2005 0.0109 0.06 0.009 07/18/2005 0.0150 0009 0.026 07/19/2005' 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 0.014 07/21/2005 00242
. -I0.026 0.009 -
07/22/2005 0.0238 0.0A 0.018 0.014 10/30/2005 - 0.0068 -
10/31/2005 0.0112 -
11/01/2005 0.0104 - -.-
11/02/2005 0.0104 . :
11/03/2005 0.0117 - :
11/04/2005 0.0165 - -
11/14/2005 0.0274 .
11/15/2005 0.0256 -
11/16/2005 0.0234 11/17/2005 o.o0.0231 11/18/2005 0.0200 11/19/2005 0.0116 Page20 of 102
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser. (Outfall.101) Discharge Concentrations of Chemicals. Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 7
Date jSodlum Towerbrom PCL-222K PCL-401 Cuprostat-PF 14-13W Nalco H-150M MSW 2Hypochlonite mg/L 7:mg/L I mg/L mg/L img/L 73551 . mg/L 101 ing/L TRC Phosphate Copolymer Azole Quat mg/L ,Quat. mg/L TRC EO/PO-_ . .. . : Phosphate 11/12/2006
/ 0.0055 11/13/2006 0.0068 - - 0,037 .
11/14/2006 - 0.0143 -- - 0.037 0 11/15/2006 - 0.0068 - , -- 0.037-11/16/2006 - 0.0267 - -- 0.037-11/17/2006 - 0.0222 -
11/26/2006 .- .0.0188 --
11/27/206 - ', 0.0138 -
1 11/28/2006 0.0120 20 11/29/2006 w . 0.0288 -
11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 ' - - - -0.015 05/29/07 - - -- .06, 0.015 05/30/07 0.0084 - <7-0.017 0,036 0.015
-05/31/07 0.0103 - ~- 0.036 0.015 06/01/07 . 0.0164 -- 0.017 ~0.036 0.015 06/02/07 0.0305 -- : - -0.015
.12/02/07 0.0241 12/03/07 K - 0.0128 12/04/07 0.0238 .
12/05/07 '0.0158 12/06/07 0.0162 12/07/07 0.0175 -
.04/13/08 .,0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.0149 10/26/08 K0.0260 10/27/08 0.0151 0.011 -
10/28/08 .w 0.0172 10/29/08
- 0.0154 004 4.000.0180.30 10/30/08 K - 0.041 0.030
- 10/31/08 0.0086 0.041 0.030 Page21 of 102
Table B-i: Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -November 20, 2009
- Date.'... T6o6'ebf6 POCL-222 PCL-401'_ CL3836) Uposto~afPf H-130M ' Nalco- H-150M MSW mg/L mg/-IL mg/L ing/L mg/L. mg/L< 73551 'mg/LJ 101 TRC. Phosphate Copolymer. DMAD Azole Quat mg/L Quat mg/L EQ/PG Phosphate 02/08'/09 0.0197:- - 0017 . -
02/09/09 0.0237 -- 0.017 -
-02/10/09 0.0104 !**i ,:**(.'::!::*.*:.:;0.021 0.0155 * *~i!0.017 02/11/09 02/12/09 0.0q106 ".0.017 02/13/69 05/10/09 0.0129 05/11/09 0.0053 0.04150 05/12/09 0.0396i'::i 05/13/09 0.0049, *** , :;; ;. R0396*.**i:i:*
05/14/09 <0.0141 0.39 05/15/09 0.025 ::i** *!:i *-* i " ": '*
11/15/09 11/16/09 0.0152
- 0.0255 "*:* *;*i!iii !i *, g!:.* i ;.*
11/17/09 0.0306:
11/18/09 11/19/09 0.0093 ** *i:;;,i - * ;! **.! i;*~i**!:*i iii!:}
11/20/09 Page 22 of 102
Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 102
BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of 1 e*ent: TVA Enviromental Testing Solution, Inc. Delivered By (Circle One):
P;ect Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS. Bus Client PV. Number: N/A Asheville, NC Other (specify): Express Courier 28801 General Comments:
Facility Sampled: Sequoyah NP
__________________________Adam Deimling NPDES Number: TN0026450 Phone: 828-350-9364 Stephen Williams:
Collected By: Adam Deimling & Stephen Williams Fax: 828-350-9368 Samples remained on ice throughout sampling and transport to lab. Dissolved Metals sample filtered and place on ice.
Field Identification/ Grab/Comp Collection Date/Time Container Flow' tit* 5S12l Sample Description Number & (MGD) Rain Event?
Colume (Mark as Appropriate) C A \i..
Date S Time Yes If-Yes, o Trace ET':Lo* , Ar.iVa Temp.. Time,.,Appear-(m/d/y (S)Inches :~Number. ac SQN-l10 1-TOIX A Comp 11/15/09 - 0944 - 1(2 5gal) 7b.3q 11/16/09 0844 _ _ ______ ______ o._._
SQN-, 0 l -TOX B Comp 11/15/09- 0944- 1 (2.5gal) 3 - ____ ___ ___ ______
__________________ 11/16/09 . 0844 /-D.
SQN-INT-TOX Comp, . 11/15/09 - 1014- 1 (2.5 gal) NA4.1 11/16/09 0914 Sample Custody - Fill In From Top Down -* "'vitc,
- m f" T ALt. &IAW14E Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Adam Deimling /12- 30 ).'O r / Express Courier . ,,. //2-!& O Express Courierf LE- ________t-lO S 3 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C, and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way'that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page_1_of 1 Cint: TVA' Environmental Testing Solution, Inc. Delivered By (Circle One):.
Pfject Name: Sequoyah NP Toxicity 351 Depot Street. FedEx 'UPS Bus . Client PV. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 GeneraleCmments:
________________ _______________Adam Deimling _ _ _ _ _ _ _ _ _ _ _
NPDES Number: TN0026450 *Phone: 828-350-9364 Jonathan Walker :___, _______*
Collected By: Adam De-lig & Stephen Williams Fax:. 828-350-9368 Samples remained on ice throughout sampling and transport to Clab. Dissolved Metals sample filtered and place on ice.
Field Identification / Grab/Comp Collection Date/Time Container Flow Sample Description Number &. (MGD) Rain Event?
(Mark as Appropriate) -ao try U>A Se Volume -
Date Time If Yes, No Trace -ETS "o, Arrival Temp- By Time Appear (mm/dd/yy) (EST) Inches Number (1c)
SQN-101-TOXA Comp 11117/09- 0745- 1 (2.5gal) v .7 1 11/18/09 0645' _0 _3 1. IC SQN-I01-TOXB Comp 11/17/09- 0745= 1 (2.5gal) R't.7" , * %it 3*
.__11/18/09 - 0645. 0.-._.___
/is. \. !S* )., %.
SQN-INT-TOX Comp 11/17/09 - 0805- 1 (2.5 gal) NA 11/18/09 0705 0,16 : A'4ý :
Sample Custody - Fill In From Top Down '"0174 Relinquished By (Signature)'. Date/Time Received By (Signature): .
%A- at-440-#
taie/Time
__________________________4 _ ________1Ae.-r-Md Afs#, ALL sAMPyte.i Adam Dei ((hng3j Express Courier 6/ý 2 ]0r- 1/id?
Express Courier4 *, t9-lt'(A ISo e" ETS It-c--it. j Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. .Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for, Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY-RECORD Page l of 1 nt: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):.
Piwect Name: Seqtioyah NP Toxicity 351 Depot Street. FedEx UPS Bus - Client Pt Number: N/A Asheville, NC Other(specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:
_ _ _ _ _ _Adam Deimling NPDES Number: TN0026450 Phone: 828-350-9364 Stephen Williams:_ ________________
Collected By: Adam 1eimling & Stephen Williams Fax: 828-350-9368 Samples remained on ice throughout sampling and transport to lab. Dissolved Metals sample.filtered and place on ice."
Field Identification / Grab/Comp Collection Date/Time Container Flow Sample Description - Number & (MGD) Rain Event?
Volume (Mark as Appropriate) Lýboiatlory Use Collected l,! i:*-'iiii : ;! i-i i[l.i, *i; :% i7 i11 :*7;1 . I'iii;i 1141':
Date Time iYes If Yes, Trace ETS Log Arrival Temp. By, Time Appear (mm/dd/yy) (EST) " Inches ........ Numbe. CC. a..ce, SQN-10I-TOXA Comp 11/19/09- 0735- 1 (2.5gal) ,
_ 11/20/09.
!__ *0635 1760.
SQN-101-TOX B
' " '11/20/09 Comp 11/19/09- 0735- 1 (2.5gal) I76*V6 063520. '&
04112604:,*::*;!*:,.*;*:i:;#
0q ,i i** ~4'4O !
SQN-INT-TOX Comp 11/19/09- 0756- 1 (2.5 gal) NA I.
11/20/09 0656 lII2Dp Sample Custody - Fill In From Top Down "4 Cu s..r Relinquished By (Signature): Date/Time Received By (Signature): D Tue z-¶ mwir
. ;,,a *.ta.sh.A IL Adam Deunling
-- (~ -C c~6~ r Vxpress Courier e6 Q 6'91n E Express Courier / t TS Q) /~gq Instructions: Clients'should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way.that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or.UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 1 of 6 "ETS Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0). *:*A o Species: Pimephales.promelas Client: Tennessee Valley Authority CountY: R- ea4 FacilitY:. Sea uoyah, Nuclear Plant A Outfail: 101 NPDES #:.. TOOZ6'I 1OaO46"- SO 'I" Project N;: 5' L.
Dilutionpreparationinformation: Comments:
Dilution prep (%) 11.3 1 22.6 45.2 72.6 100J uent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (rmL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:
Organism age: ,"
-1:5 o
" 0o.vs Randomizing template: 4peo.lul Date and times organisms \N-t11.O' %aCO Incubator number and were born between: shelf location:
- Organism source: 6M% . -'xq X 1\-O' Artemia CHM number: JA*I'q%
Drying informationfor weight
___determination: _
Transfer bowl. information: pH = ".St S.U. Date / Time in oven: 1" .O*
Temperature =1A.'10 C Initial oven temperature: 6tvC Average transfer volume: Date / Time out of oven:
- 03. LIPOS Final oven temperature: 1.0 "¢-
______. ________ - _______Total drying time: ___.___
Dailyfeeding and renewalinformation:
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analy st Outfall 101 Intake 0- -4OO - 0S oat t % " ll I-dk Pi 4 ~ rio (H__00~ ~(~j VA' Do~ aiIl1 101%OhvI.os' I t 5
. w_ nk nn
- A I-v.,--, -
A4
- ~~I~4~-I ncil-7n,-q,7 IAOW Iln - C 4 - -
it. -0i 1:541 4A
. Control information: Acceptance criteria Summary of test endpoints:
% Mortality: 07. 1 !20% 7-day LCso ! 007.
Average weight per initial larvae: .I1.I'L- NOEC OO.7 Average weight per surviving larvae: -0. I L. - > 0.25tg/larvae LOEC )1007.
ChV >1007.
IC25 too7v SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page27 of 102
- Page 2 of 6 F S Environnwltal Testing Solutions, Inc.
Species: Pimephalespromelas -
Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date: i - i"
-Survivaland Growth Data Day CONTROL 11.3% 22.6%
Non-treated
__ "__ A B C D E F G H I J K L 0 to. 1O 10 I0 10 *iQ i0 1o 0 10 to I__ _ to 10 iO 1t /o / /0 i / 0 10
/0 /0 2 JQ1 1 10 (0 /to10 t 0 ;O I0 to 10 IIo ) 0 10 t0o I 10 /0 to,. /0" /b 6 /0 (0 10 ID /0 10 10 10 /0 10 10 0 5 toui 10 10 10 10 I O /o0 1t0 10 !0 6 ).t1 I r 1 o o1 o I ,1O 1 11(0 to/0O 7_ 10 ./O t) 11 10 t to 1 0 10 /0 /0 A = Pan weight (nmg)
T ra y co lo r co d e ::, Ih A L I .3; " .3
" ( 1 Analyst: t~c 3D (* 1.& 9i. ~ r . 4 -1 qq~q~
Date: 10 -.Aj -2, B = Pan + Larvae weight (tu g) 2%
Analyst:, j 35 ZL-u zz. ? z %.5 zz 2Z-3ýo Z34 1 Date: )z .,-jo C = Larvae weight (mg)
Weight per Initial number of larvae (mg)
N%
= C / Initial number of N~
o" NN larvae O' ci ly Average Percent
- 0. , - " -1.* - .
weight per reduction a.'
initial from number of control (%)
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m - missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
IComments:.I Calculations and data reviewed: 4 -
Page 28 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I Page 3 of 6 I DTS EnvimnmmtAstin T SolutnaonsInc.
I Species: Pimephalespromelas"!
I Client: TVA /Seauovah Nuclear Plant. Outfall 101. Non-treated
_______________ ________________Survival and Growth Data Date:
!1- I- CA 42%.Day 72.6% 100%
____ M" N 0r P R S T U V W X-1t I*o I 1o tO t It " 10 10 to 0 10 ID t.6 to '0 /0 '/0 /0 /0 /0 /0 /0 /0 4(0 /O 0 /0 ftO /0 /0 10 10 it t0 5
""_ _ JO O 0 o10.Ii /0 1 10 /0 to to lo IFo 1.0o to 0 o /Mo /0 10 10 10 toL)10 10. 10 It) 10 10 to 10 0 1 A Pan weight (rag)
Tray color code::
Analyst: ..l" I - "I1q. . II 16,S- JO1q,01.
1 i1#. 1S
" ) I,36,s,3 6 h,r I,,t.
Date: !Q - 0----
B, (rag) Pan + Larvae weight -
Analyst: *TJ 7_q 2, Zýb Oo " z~l 2\.sq z3.41q~ 1Z.iz, -L.1'Z 3 2.z,3D 0 -ý
'0z -.t4.7 Date: *7.o\-.
C = Larvae weight (rag)
-B-A - in, t . R*". .St "%.'0l" ' g6.
2,,a1-. z. ( -1.1 Weight per initial number I " --V *V
= C / Initial number of larvae I Average weight per initial number of Percent reduction from control M%..
0.A t.
- 0.-(67 0. . ,, -1 J. 7.
larvae (mg) V Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded.
I Calculations and data reviewed:
SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 29 of 102
Page 4 of 6 FETSý,
0
') E~wfon,,,.taI Te tinSohttonnc.
Species:-pimephalespromelas Client: TVAI/ Seauoyah Nuclear Plant. Outfall 101. Non-treated Date:
- 01q.j
. * " ' _ .. . .t .L Day . 100% Intake Y z AA BB 0 o t(0 ',
/~0 2 "z 2 /Q '0 o -
__ __ _ __ _ 10 10 10 10 3i* i o '0'j 4Lt0 1 6 t-7 A =-Pan weight (mg)
Analyst: s _
Date: coo- c -4) -0 9 B = Pan + Larvae weight Analyst:. . _.,)
Date: %"ZNO',Vizl C - Larvae weight (mg)
=B-A . m .0 %
Weight per initial number of larvae (mag) 0.
= C / Initial number of larvae g.
Average .Percent weight per reduction initial from control 0.1 1( 7.
number of (%)
larvae m Comment codes: c = clear,.d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w wounded.
Calculations and data reviewed:
Comments: i Page 30 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24,2009 PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc. Prject numnber: 5812
~B
~~zd.lohush~e C na naoatlua(%) Bnpl lr o [nlalt .as mb w F ho va. A - P- s LN ot fo r C' cc I LOs %,. I-. I÷ t E t
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tIsa* ~ A-- ~ h1W ~ ~- hWgkt(.-o
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- Wna .
mber, .
ofrarhlo.
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ouallbrvrat',ae Hue (%)
earotodt vo'h-n.
A.srd ,
ofa Pt-t .- ed (%) k A 10 10 13.60 21.90 8.30 0.830 0.830 R
B 10 10 14.25 22.42 8.17 0.817 0.817 Control C 10 10 13.11 21.53 8.42 0.842 0822 2.3 0.842 1000 0S22 23 Notapplicahle D .10 10 15.55 23.52 7.97 0.797 0.797 ' . ,
E 10 t0 13.18 21.75 8.57 0.857 . 0.857 11.3%' F 10 . 10 14.47 22.67 8.20 0820 0-834 2.2 0.820 100.0 X014 -.12.2-5 G 10 10 14.12 22.31 8.19 0.819 0.819 H 10 10 13.60 21.99 - 8.39 0.839 0.839 ',
1 10 10 13,09 21.58 8.49 0.849 .0.849 22.6% 10 10 14.93 22.85 7.92 0.792 0.830 3.4 0.792 10050 a4.3 s., -I.0 K 10 10 14.42 22.36 7.94 0.794 " 0.794 "A-L 10 10 14.65 23.49 8.84 0.884 0.884 " "_'_"_ " _
M 10 10 13.13 20.95 7.82 0.782 0.782 N 10 9 14.12 22.44 8.32 0.924 0.832 8.5 0.832 9080 4.6 1.6 45-2% 0 10 10 13.99 22.48 8.49 0.849 0.849 P 10 10 11.91 19.63 7.72 0.772 0.772 _ _.__-
10 10 14.05 22.11 8.06 0.806 0.806 72.6% R 10 10 14.01 21.59 7.58 0.758 0.779 2.6 . 0.758 0.770 100.0. 0779 2.6 5.2 S 10 10 15.77 23.47 7.70 0770' T 10 10 14.91 22.72 7.81 0.781 0.781 _ _ _ __,
U 10 10 13.61 22.13 8.52 0.852 0.852 V 10 10 14.38 22.30 7.92 0.792 0 0.7 100% 10 13,83 20.80 6.97 0.697 0.697 1 ,
W 10 X 10 10 15.26 22.42 7.16 0:716 0.716 ,_ . _
Y 10 " S 14.87 . 18.4 3197 0.794 0397 1010 10 13.41 2.1,9 7.78 0.778 0.816 6.1 0.778 875 0.716' 30.5 12.8 AA .10 104.49 22.50 8.01 0.801 0.801 B9l 0 44 23.29 8.89 0.889 0.889 Outilall 101: MSD = Minimum Significant Difference Dennett's MSD value: 0.0683 PMSD = Percent Minimum Significant Difference PMSD: 8.3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declara1 statistically significant in a whole effluent toxicity te.
Intake:
Danuett's MSD value: 02577 Lower PMSD bound determined by USEPA (I0th percentle) = 12%.
PMSD: 31.4 Upper PMSD bound determined by USEPA (90th percentile) = 300.W Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interluhoratory Variabiliy Study (USEPA. 2001 a;.USEPA, 2001b).
20 USEPA 01a, 2001b. Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods%Volumes I and 2-Appendix. EPA-821-B-0I-004 and EPA-821-B-01-005. US Environmental Protection Agency. Cincinnati, OH
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated 0
November 17-24, 2009 Statistical Analyses t~J SEnvironmental Testing Solutions, tnc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/17/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitorilg'Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8300 0.8170 0.8420 0.7970 11.3 0.8570 0.8200 0.8190 0.8390
.22.6 0.8490 0.7920 0.7940 0.8840 45.2 0.7820 0.8320 0.8490 0.7720
- 72.6 0.8060 0.7580 0.7700 .0.7810 100 0.8520 0.7920 0.6970 0.7160 Intake 0.3970 0.7780 0.8010 0.8890 Transform: Untransformed I-Tailed Isotonic.
Conc-% Mean N-Mean Meaný Mm Max CV% N t-Stat Critical
- MSD ,. Mean N-Mean D-Control 0.8215 1.0000 0.8215 0.7970 . 0.8420 2.345 4 . 0.8283 1.0000 11.3 0.8338 1.0149 0.8338 0.8190 0.8570 2.162 4 -0.432 2.410 0.0683 : 0.8283 1.0000 22.6 0.8298 1.0100 0.8298 0.7920 0.8840 5.397 4 -0.291 2.410 0.0683 0.8283 1.0000 45.2 0.8088 0.9845 0.8088 0.7720 0.8490 4.641 4 0.450 2.410 0.0683 0.8088 0.9764 72.6 0.7788 0.9480 0.7788 0.7580 0.8060 2.626 4 1.509 2.410 0.0683 0.7788 0.9401 100 0.7643 0.9303 0.7643 0.6970 0.8520 9.351 4 2.020 2.410 0.0683 0.7643 0.9226 Intake 0.7163 0.8719 " 0.7163 0.3970 0.8890 30.456 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98188138 0.884 . 0.392664346 0.32317638 Bartlett's Test indicates equal variances (p = 0.14) 8.39288521 15.0862722 Hypothesis Test(l-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE ,., F-Prob df Dunnett's Test 100 >100 1 0.06829131 0.08313002 0.00325397 0.00160593 0.123331696 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(Exp) . Skew IC05 65.141
'dIo >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnlOJJJ-17-O9data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake:,.,
Non-treated November 17-24, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:. 11/17/2009 TestID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821 -R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8300 0.8170 0.8420 0.7970 11.3 0.8570 0.8200 0.8190 0.8390 22.6 0.8490 0.7920 .0.7940 0.8840 45.2 0.7820 0.8320 0.8490 0.7720 72.6 0.8060 0.7580 0.7700 0:7810 100 0.8520 0.7920 0.6970 0.7160 Intake 0.3970 0.7780 0.8010 0.8890 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean - Min Max CV% N t-Stat Critical MSD D-Control 0.8215 1.0000 0.8215 0.7970 0.8420 2.345 4 11.3 .0.8338 1.0149 0.8338 '0.8190 0.8570 2.162 4 22.6 0.8298 1.0100 0.8298 .0.7920 0.8840 5.397 4 45.2 0.8088 0.9845 0.8088 0.7720. 0.8490 4.641 4 72.6 0.7788 0.9480, 0.7788 0.7580 0.8060 2.626 4 100 0.7643 0.9303 0.7643 0.6970 0.8520 9.351 4 Intake 0.7163 0.8719 0.7163 0.3970 0.8890 30.456 4 0.961 2.353 042577 1 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.82512182 0.749 -1.7159101 4.30798649 F-Test indicates unequal variances (p = 2.30E-03) 128.264832 47.4672279 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Heteroscedastic t Test indicates no significant differences 0.25768323 0.31367405 0.02215513 0.02397863 0.37355912 I, 6 Treatments vs D-Control sqnlOJ_11-17-09data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated November 17-24, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0
ýý FS *Daily Chemical Analyses
"' Environmental Testing Solutions, Inc. Project number 5812 Reviewed by:
'Concentration Parameter DDIy_5a pH (SUI) 7.481 7.50 7.631 7.41 7.501 7.44 7.711 7.56 7.55 7.0 7.421 7.231 7.79 7.24 DO (mg/L) 7.81 7.5 7.61 7.3 7.71 7.51 7.81 7. 7.9 .5 7.81 6.91 8.0 7.1 Conductivity jI~mboalcm) .32 332ý Control Alkalinity (mg/L CaCO )
3 Hardness (mg/L CaCO 3) 90 Temperature CC) 24.8 24.6 24.8 24 24.8 24.7 24.8 24.7 24.7 24.6 24.8 24.8 pH_(SU) 779 7.46 7.66 7.37 7.5 7 7.731 7.50 768 71 757 720 762 715 DO 80 7.3 7.7 7.3 7.7 778 69 80 71 11.3% Conductivit bo/cm) 317 3138032
- _ _ Temperature CC) 250 24.6 24.9 24.9 24.9 24.7 24.58 24.7 248 24.8 24.8 pH_____(SU)____ 7.74 7.44. 7.624 7.35 7.56 73 7.72 7.5 765 717 53 7.11 7.61 7.14 DO (
22.6% _____g___ 8.0 7.4 7.81 7.1 7.9 7. ~ 8.2 76 8.0 73.9 638.1 6.8 22.6% Conductivity (guhos/cm) 299 293 2682__8 rem____ 250 24.8 24.9 24.9 24.8C 24.8 24.9 26448 2482847 P(S)7.73 7.44 7.61 732771 71 733 5 62 716 ,47 715.59 71 "45.2%_DOL1 74 77 7 45.2% Conductivity (guhos/cm) 266 ____ __24__25 254 Temperature(C) 251 249 24.9 2 249 249 250 2498 2477 24.6 24.9 7H 7SU43 759 7248 733 767 75 760 7241 715 756 72% DO (mg/L) 8.1 7.3 7.7 70 7.9 7.1 821 78 8.3 74 8.2 6.6 8.2 6 72.6%_
(I____m Condutivit 25 2 27_1 217 211-Temperature CC)_________ 25.1 24.8 24.9 24.9 249.25 25.01 24.81 24-8 24.7 247.26 24.91 24.51 Conductivity (junbos/cmr 100% Alkalinity (mg/L CaCO3)
Hardness (mg.L CaCO 3)
Totai Residual Chlorine (mg/L) <10 Temperature (C) 25.3 25.0 2 25.0 24.7 25.1 246 25.0 247 247 24.5 24.9 24.7 PH_______________ 7.571 7.431 7.571 7.3 7.42 7.33 7.64 7.49 7.52 7.07 7.261 7.041 7.471 7.011 DO ( _____________ 8.31 7.61 7.81 . 7.2 8:11 7.41 8.21 79 8.1 7.51 8.41 6.51 8.21 6.5 Conductivity (lunhos/cm) 100% Intake Alkalinity (mg/IL CaCO3)
Hardness (mg/L CaCO3)
Total Residual Chlorine (mg/LJ <0., <0.0 <0.
_ Temperature C) 25.0 24.7 25.0 25. 24.8 24.7 250 246 25.0 24.6 2.9 24.6 24.9 24.7
.0 Page 5 of 6 0
tnvironmental T l utions, Inc.
Species: Pimephalespromelas.. Date:
Client: TVA / Sequoyah.Nuclear Plant, Outfall 101, Non-treated Dallv Chemistrv:
.... ..... -:' Analyst Concentration Parameter Conductivity CONTROL (tImhos/cm)
Alkalinity Non-treated (mg CaCO 3/L)
Hardness (mg CaCO3/L)
Temnerature (0 C)
I 9 pH (S.U.)
DO (m2/L) 11.3% Conductivity (Ltmhos/cmý TemDerature (00 I I pH (S.U.)
I 1F,(o II q.?~3 I DO (mafL) II ':3., I 22.6% Conductivity (iimh s/cm) I __
-C Temperature (QC) L5.O *,-s 0 4
DH (S.U.) II '.*,t4LI-I 1;.I II II 7.132
- 7 I "1.S1 DO (mpL) 6.I 45.2% Conductivity (A+/-M OS16m) ____
Temperature (M) ASA I -a Gi I M -Cl IM . 0 1 Vk .11 1 _1J4 -,A I pH (S.U.) lr4,,, 1*14 8, DO (mn/L) PAO 1.4 72.6% Conductivity (4MhosIm A2 I Temnerature (°0 "LS zt pH (S.U.) 4, (.d,4 0 11 j 3q -7 . .
r DO (mg/L)
Conductivity u Alkalinity 100% (mg CaCO31L)
Hardness &2 (mg CaCQ 3/L) ____
TR chlorine (mgfL) -Co. 1c0 Temnerature (0 C')
Teprtr *.C).
oH (S.U.)
0~
DO (mg/L)
Conductivity (umOs/cm) 100% Alkalinity Intake (mg CaCO 3/L) ____6_
Hardness (mg CaCO 3/L) ____
TR chlorinel(mg/L). 40.1I Temperature (QG)
U U Initial
,, U *
- SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 35 of 102
Page 6 of 6
- Sliecies: Pbnevhailes gromelas HI Clienti .TVA/'S eiuovah NuclEar Plant; Outfall 101. Non-treated - Date: I\
I Dav .. :"*'- I
. I... ~-L& 6."
Analyst . .. ,L .I :t 4
.. gL.ad-.
vI~r l
f~Y- 5 SII :! I:
,Concen- [Parameter tra-tion ________
pH (S.U.-)
DO (mg/b) I 1 1I I 1.9 3-4 .13 Conductivity CONTROL -(gmhos/cm) '3 )1- .33-Alkalinity Non-treated (mg CaCO39L)
Hardness 334.'I 1, Tk-2 1 (mg CaCO3/L) ____ iA.1 Temperature (M) i a -
oH (S.U.I a-DO (m/UL) 9117- .0 11.3% Conductivity (Ismhos/cm) 50"T ý03 Temperature (MC) -L-A --I OuA - -Lk .4 1A.9 a a oH (S.U.) L 1 -7.50 -7.LOS 1 0 4- 1 __3 25 3 11 11, DO (me/L) 22.6% Conductivity -
(Jimhoslcm) I I,.-rIL4'iA U Temperature (M) "Mq.i t I 1A. a a a -- ** -
nHl (S.U. cL-~i I7&~- i -i~iI i-i~ I~-v II~ I1, -q II'
[
,pH-- (S..../
DO (mg/L) 45.2% Conductivity 1 -71.1.11A1 MIA (grmhos/crn)
Temperature (°C) -lus LU Teprtr -, I-pH (S.U.)
F IýIlq 7. *
-kao - In
-04_L
-- "--
- 1T-]'41;TI In ! 1~T Z - - -- II 404 II
- A I
DO (mg/L) 1 8.3 .1 q-Q 9.2 -11 U.(,__ F 72.6% Conductivity 7113 All p
Temperature (°C) "LS .0 11 I -
1 .1tw 1" 'AM, -Lk DH (S.U.) /3 IA:1ý F -~.'=.~--l*
11 -7.4 1A 1 1.61:)
11.4.001 1 DO (m/bL) 9.0 T F.
Conductivity (jimhos/cm) Lou Alkalinity 100%
(mg CaCO3IL)
Hardness (mg CaC0 3/L) ko Ck TR Chlorine (mg/L)
Temperature (00 I-------------- --------- -- I - I -- 1- - I pH (S.U.) I ]~c~ Iic2. 1I I1 -. V- 14qq- 9.01 - 1J DO (mg/U %04 AA7 I I.r,7-Conductivity (pmhos/cm) 100% Alkalinity Intake (mg CaCO3/L)
Hardness (mg CaCO 3/L)
Temperature ('C) 11t.o Fin.a.. I-tl I I 1.14.,l1*(+II I l14M II tI I
- U
- l Initial Final Initial " Fi I 3Initial Final I Initial Final I
&MMEEMMM....WMAN SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 36 of 102
I S Page Iof7 I
C'hronic, Whole Effluent Toxicit Test (EPA-821 R-02-6013 Method 1002.0)
Species Ceriodaphniadubia ,
..Client: Tennessee Valle6. Authorit . Count:Hamiton ,
Fa0cility: SeauovaluNitile Plant -Outfall: 101'.: ,
-NPDES #:TNO026450 Project #: "-&VL.-
Dilutionpreparationinformation: Comments:
Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organismsource information: Test information:
Organism age: < 24-hours old Randomizing template color: MA.YME Date and en: times organisms were born -T,0 V740 Incubator number and shelf between: location: 2.6 Culture board: A.
A-,l-o _
Replicate number 1 2 "3 4 5 6 7 8 9 10 Culture board cup number:-. 6 11 1 , Ot 101 IN%M I, 'll I% YWT batch: 1*r*O*
Transfer vessel information: I pH S.U. Temperature =*,S. 'C Average transfer volume (mL): I C,, PAe* I n..bc Daily renewalinformation:
Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfail 101 Intake termination time 0 i1-'O ts)( it-r.4 a*.-M0 k C iI OS I,10.
0 llll-a SOi Aý0j.11 nlU.-0" Oa IM-0.O 3 IV- VAIitI . 05 0-%%11cr 4.04 A Ivl0i:& __________f-lA 01W1.04 OR~~ M oa 4 6 .l.. . iio f i \- -0 1 .'-,-. A'J ' OS IV &WJI ~5I"l, 7 1 it- 14 A. 1 14 0 -L-Controlinformation: Summary of test endpoints:
_ Control-I Control-2 " Acceptance criteria
% of Male Adults: .,20% *. 7-day LCso > 1001.
% Adults having 3 ' Broods: - I . /o07, k 80% NOEC IOG1,
% Mortality: , . 520% LOEC w7.
Mean Offspring/Female: ?'C.S "2-014 k 15.0 offspring/female ChV ),107,
%CV: -'" 7. 5.qT. . <40.0% IC25 i.,
Page 7 of 102 SOP AT I1- Exhibit AT! 1.2, revision 04-01-09
Page 2 of 7 I. ETS
' Species:-C*eriodaohnia dubia.
S Client-:. TVA / Seauovah Nuclear Plant, Outfa11101 Day ".. . 1 2 3 4 5 Survival and Revroduction Data, Replicate number 6
Date:.
-T-7 8
8 11"-
"0%
9 10 2 Young produced C) C) 0 C) r) 0 0 0 3
Adult mortality Young produced __ ' .
C C U0 L0
- t L_.
0 0 i.-
)
U 4 Adult mortality Young produced
- t U ..
U .- U-6 Youngproduced 0 I 0 I t . 0 . IC 6 Adult mortality Young produced 0- \ C) - C\ \ cL U.. o LiS " - b)..
U 0i.
= U.-
Adult mortality ~ (~ U ~ - \
7 Young produced I$ , ... - L 1' Total young produced ,, I 2-" 3 7.
I. Final Adult Mortality *C L. s.- - U X for 3' Broods " "> .- ". I " .Z.-
NoweAdult motoality (L live,D-dead).SB splitbrood (siogle broodsplitbetwjeen twodayo), CO=conry over (offspring cantied overwithAdult durntgtroansfer):
Concentration:
% Mortality:__________
- n " Mean Offsrin*e*male:
Sow: 11.3% , Survivalnd Reproduction Data S .Replcatenumber I Day 1
R "I
Young produced Adult mortality
. 1 10 L
I 2
L! .
c)
U 3
I 4 5 0 -
U..
a-6 L-7
- 8 9
=
10 L-I Young produced 0I .l I o I .
Adult mortality I I U ! U ! U 3 Youngproducedi 0 Il 0 I 0C)
Adult mortality U -, I " -- U. ---
- I-4 Young produced IS '
%A 4 1 q S S 4 4 I Adult mortality Adult mortality l-U L= U
'L... -U %--
t-Il1 U L-U I U tIU U 6 Youngproduced i> "0 I" l~ t IC) In ! C Adult mortality 6 Young produced I - .QI _ I . . " 1 Total young produced 2Q , ' , t2.& z. !
Final Adult Mortality Mote.,Adult mortality (L - live, D - dead),
SO splitbrood (oingle broodspli between twodays), CO- a-~ovea offsot carnied ov% tl dl otgtaar SConcentration:
% Mortality: j Mean Offspring/Female: .- 0
[. Page 3 of 7
- ETS J
. Species: Ceriodaghniadubia S gClient:TVA Seauoyah Nuclear Plant. Outfa ll 101 .. Date: I-k *-o I Vurvival aind Ri'nrnduction Data o'.licate number ,
Day .:. ... . 2 3 4 5-, . 6 ,"7 .8 9" J. 10 Young produced C
- Q. Iý .) (
'Adult motlty _ T. __.z.
Adult mortality . * *.. k._..
4 Young produced fl *__ '." ... .. Q..._ k" -*kj Q ... .-..
3 Young produced I" C Adult mortality L_ '._, .
- I -
4 Young produced Lk \_
S-.
Adult mortality %L._ 1._..
5 Young produced t'. . L I' L [ lI
- Adult mortality \ ._ L a s t__ .
Total young produced B -..
Z _1'.2 t ,.* . .7- _%7
- B2 _5 . .32- 2--i a A*
minal Adult Mortaliy *.-- I- - Z L.. -
Mote: Adult mortality (L live, D - de*ad), SB -split broo (single brood split between two days), CO can ame (offspringt carried over"with adult during transfer),
SConcentration: -
%Mortality: 67.7U
- _Mean
% ReductionOffspring/Female:
from Control- 1:
3". 17.
CONe: 45.2% Survival andReproduction Data Replicate number.
Day ,1 2 3 4 5 . 6 "7 18 9 " 10 Adult mortality 1 _-
7 Young produced L3q ' tA I2$V3 0)I 0.
Adult mortalty *_. ._ *_ "l_ *5 * ._ *. *. k_
4o. Young produced 0 ) . 31. " ..-
Adult mortality , . 1 * . * . _.
4 Young produced Adultmortality lS *
- .L- l ' ~ _ LI ' "
\:-*
6 .Yrung produced U Adult mortality 7 Young produced t1' Total young produced 3 - _44 2 3 0,3. 0( 0, al Final Adult Mortality C I L- C" L" I " .
Note: Adult mo (L rtality
- Lim, - dead), SB - split brood (singe brood split between two days). CO- anw over (offip Ung carsed over yith adult during transfr).
I Concentration: I
%Mortality: 0).
Mean Offspring/Female: ,3.L%
%Reduction from Control-1:1: -t.
Page 39 of 102 SOP ATI 1 - Eihibit AT 11.2, revision 04-01-09
Page 4 of 7 TETS Species: Ceriodaphniadubla
. Cient:
. TVA / Sequoyah Nuclear Plant. Outfall 101 Date: I .I-I1- 05 CONC: 72.6% , Survival and Reproduction Data
_____" ! -, Replicate number Day 1 2 3- `4 5 6 7 8 9 10
- . , .young prq!Fodued ... (
0:C i.*i.0* 0':"( C) (*-
'2'- -", -ded -n I-4 Youngproduced Adult mortalityý Adult mortality
- I . .c .
, , 0......
Adult mortality _ _
.3 Young produced \ 10. 2. I* "' \ q (2)l.
mortality_..
producedAdult YuAutmortality pocYoung t __ ~ t_ S _
5 Young produced VL If lt%%k VL Vb %- V6 11I 11 Adult mortality LL.~ ___
6 Young produced 01 C) 0 00 Adult mortality -IZ KJ C:)-\..-'.
7 Young produced V0 t I'-1 Totalyoungproduced 3?- ,i 3. 3 ,4 3o, 3 3Z,-
Final Adult Mortality 'L_ ' \ - - C I[
Note: Adult mortality (LC live, D - dead), SB - split brood (single brood split between two days), CO = loiffiping carried over with adult durinn transler).
oarover Concentration:
% Mortality: '
Mean Offspring/Female: I .'1
% Reduction from Control-: 1: 7-CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced _ ' p Adult mortality L !.C_- ._. IU, .
2 Young produced _ _ -
- 0j-l Adult mortality L L. L j
! 'L-3 Young produced .,_
Adult mortality C.-C- " j 5 Young produced 'T ' Lam to'L SAdult 5 mortality Young produced I* I~* iL
.. I,* *. ~-
- t.. . _ *-
Adult mortality ~ I C. C - - - -
6 Adult mortality Young produced C) I-C_. - I.. rýQ *_ % C-8C) . '"-C. C)*
6 Young produced , ' 0 z- (0 I p Total young produced ,3 _N6 3i. S 6. 314331. 3 9 a t. a, a xf Final Adult Mortality N_.
\Z ' ,. .
- I C-t 1110-ty %L 1-'. 080),. -5pt roo.ks.%d.roousp1._ twoGyj, Concentration:
% Mortality: 7.
Mean Offspring/Female: 3 .9
% Reduction from Control-I: -
Page 40 of 102 SOP AT 11- Exhibit ATI 1.2, revision 04-01-09
0Tr5- Page 5 of 7 il ,,-.'..Species- Cerlodaphniadubla Client:. TVA SeqnuvihNuclear Plant,.Outfall 101 Date: I %VO-0 2.
CONTROL-2 Survival and Reproduction Data
.. Replicate number U
__" : f_- 2 4 5'... .6 .,7 8 9 10 I 2 Adult mortality Young produced U.-. U U U C)
'k
. U" U 'U-Adult mortality * ] ... IL ALI-..
Y Young produced [ - - - - - - , - -
Adult mortality . U ' ' U " U- U 4 Young produced . = 0A 4 Adult mortality Yong produced j-
\.- \- \-17 (0 Qj-U- %-- \I-Adult mortality C J - \,- C 6.[ Young produced 0 0 C) C) C- C)0..
Adult mortality .UaU-\ U -
7 Young produced -Lý %= -: %
Total young produced a 3c *k at) ~ .~2 Final Adult Mortality .. - U-IX for 3rrsBroods -?e,*- * * .
No*e; Adult mortality (L = ive, D - deed). SB split brood (single brood split between two days). CO - catty over (offspring carried over with adult during transfer).
- Concentration:
%Mortality:_ _ _ __
Mean Offspring/emale: lo.01.*
CONC: 100% Intake Survival and Reproduction Data Dy1
[ .. 2 3 4,1 Replicate number 5 6-" 7- 8 9 10 S Young produced 0 0 1 U U.- U Adult mortality 0 U L. U- L Young produced 9 1 Adult. mortality . *!_ [.. *.. -- *L.9 3 Young produced 0- 0 Adult mortality \..... '- . . i._ '- U U.
4 Yo.ung produced Adult mortality L U U U.
5 Young produced 7 a - - - - - - - -I Adult mortality U T U L L C= ~ U U U 6 Young produced C)) 0 (a)Q Adult mortality \ U . ui .- L U- . -
7 Young produced 11 1l l- 13 Il Is tS LS 11
,0 Total young produced w{ " " -!sz" Final Adult Mortality U , '.. *
- _ . %.--
N .-
,,*o.,z utm y k,* -uw, - a& "? sprt k rise~ roxu SP t uwc~an two uays/, rm ca Concentrdtion:
ove% -~ Itc ~ mta J~ qta Mortality:
Mean Offspring/Female: *,*
%Reduction from Control-2: -'. [7a 1 i Page 41 of 102 SOP ATI 1 - Exhibit ATI 1.2, revision 04-01-09
TVA I Sequoyah Nuclear Plant, Outfall 101 - Non-treated 0 November 17-24, 2009 Verification of CeriodaphniaReproduction Totals Control-I 72.6%
Day Replicate number Total Re plicate number".
Day 1 2 3 4 5 6 ' 71,r "7.8" 9 10 Tt 11 2 13 4 5 6 7 8 9 10 1 0 0 0 0 0 .0 0 0 0 0 0 1 0 0 0 0 0 0 0.';' 0"o !0 '0 0 2 0. 0 0 0 0 0 0 0.0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0. 0 0 0. 0 0 0 0 3 0 0 0 0 ' 0 0 01
. ... 0 .0 -0 4 4 4 4 4 5 5 4 4 6 6 46 4 5 5 4 6 6 4 6' ,5 ' 5 74 50 5 13 10 10 12 10 12 12 12 13 11 115 5 12 12 14 12 13 13 A13:" 14 II 11' 125 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0. 0'; 0 0 0 7 15 15 12 14 12 13 12 13 12 16 134 7 16 17 16 14 18 16 19: 15i: 14 17 162 Total 32 29 26 30 27 *30 28 29 31 33 295 Total 33 34 34 32 37 33 38- 1 341 30 32 337 11.3% 100% _____,___________
Replicate number Total Replicate number Total Day I 2 3 4 5 -6 7 8 Day 1 2 3 4 5 6 7: 8-" 9 10 Total 9 10 1 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0,.-. 0. Q0.. ,0 0 3 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 *0 0 0 0 0, "0 0 0 0 4 5 4 4 4 *4 5 5 4 4 4 43 4 5 6 5 6 5 5 7 5 .6 6 56 5 11 13 12 13 13 12 12 10 12 12 120 5 14 12 10 13 13 12 15ý 11, 14 14 1 128 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 14 12 14 13 13 13 14 13 17 139 7 16 18 17 17 16 19 16. 20M 18 18 175 Total 32 31 28 31 30 30 30 28 29 33 302 Total 35 36 32 36 34 36 381 '36 .38 38 359 2 2 .6 %
Control-2 -_______
Replicate numberToa Day 1 2 3 4Replicate 5 number 6 7 8 " 9 10 Total Day 1 2 3 4 5 1 6 17 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0" 0 .0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0' 0 0 0 4 4 5 4 4 6 4 4 4 6 5 46 4 4 4 6 4 5 4 4 4 4 43 5 14 11 12 12 12 12 1l' 13 13A 1 121 5 13 9 12 12 11 13 12. 13ý .12 10 117 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 F 0 .0 0 0 7 14 16 13 16 14 1617 15 13 18 152 7 14 14 12 16 13 12 15 13 15 14 138 Total 32 32 29 32 32 32 32 32 32 34 319 Total 31 27 30 32 28 30 31'. 30 31 28 298 45.2% 100% Intake*
Day Replicate number Total Replicate number y1 2 13 4 5 1 6 7 8 9 10 Day 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 00 0 0- 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 .0. 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 .0 4 5 .5 6 4 5 "6 5 5 4 4 49 4 5 6 5 4 4 4 '.-5 4' '.4.-, 5' :46.
5 13 14 12 10 12 12 12 12 12 "13 122 5 14 12 11 12 12 13 12 12 12 12 122 6 0 0 0 0 0 0 0. 0 0 0 0 6 0 0 0' 0 0 0 0 0 0 0 0 7 15 15 15 17 15 18 13 17 17 15 157 7 19 17 17 14 13 16 15 15 15 16 157 Total 33 34 33 31 32 36 30 34 33 32 328 Total 38 35 33 30 29 ,33 32 31,1 31 33 325
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.
Project nuim bet: 5812 Rev lwedy: K LtiýI Concentration Replicate number 0 Survival
% Average reproduction (offspring/f emO le ip ,.. ]I Cofi ln aof Coefient ,Pre trd
. Percent cinffrom reduction
.1 e:va Wiation(%) pooled con trol, (%)
.4 5 6 7 8 9 10 (% ) f.rn g. -...
(% ) 1 . 2 3 Control- 1 32 29 26 30 27 30 28 29 31 33 100 29.5 , *- '7.4 Not applicable 11.3% 32 31 28 31 30 30 30 28 29 33 100 30.2 - 5.4 -2.4 22.6% 32 32 29 32 32 32 32 32 32 34 100 31.9' . 3.8 -. 1 45.2% 33 34 33 31 32 36 30. 34 33 32 100 32.8 - . 5.1 -11.2 72.6% 33 34 34 32 37 33 38 34 30 32 100 33.7 *. K 7.0 -14.2
.100% 35 36 32 36 34 36 38 36 38 38 100 35.9 .' 5.3 -21.7.
Control-2 31 27 30 32 28 30 31 30 ,31 28 100 29.8 *.- 5.4 - Notapplicable 100% Intake 38 35 33 30 29 33 32 31 31 33 100 32.5 :.8.0..9.1 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 1.906 PMSD= Percent Minimum Significant Difference PMSD: 6.5 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Intake:
Dunnett's MSD value: 1.676 Lower.PMSD bound determined by USEPA (10' percentile) = 13%..
PMSD: 5.6 Upper PMSD bound determined by USEPA (90' percentile) = 47%.
Lower and upper PMSD bounds were detcrmined from the 10th and 90th percentile, respectively, of PMSD data from EPA's'WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-AppendikxEPA-821-B-01-004 and EPA-821-B-01-005.
US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated
.t~j I
- " TS
.E
- ~ Environmental Testing Solutions, Inc.
November 17-24, 2009 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/17/2009 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 10 1 End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date:
- Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia, Comments: Non-treated Conc-% I 2 3 4 5 6 7 8 9 10 Control-I 32.000 29,000 26,000 30.000 27.000 30.000. 28.000 29.000 31.000 33.000 Control-2 31.000 27.000 30.000 32.000 28.000 30.000 31.000 30.000 31.000 28.000,-
11.3 32.000 31.000 28.000 31.000 30.000 30.000 30.000 28.000 29.000 33.000 22.6 32.000 32.000 29.000 32.000 32.000 32.000 32.000 32.000 32.000 34.000 45.2 33.000 34.000 33.000 31.000 32.000 36.000 30.000 34.000. 33.000 32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30.000 32.000 100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000 .
Intake 38.000 35.000 33.000 .30.000 29.000 33.000 32.000 31.000 31.000 33.000 Transform: Untransformed Rank 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean Control-I 29.500 0.9899 29.500 26.000 33.000 7:366 10 32.333 1.0000 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000 5.362 10 115.00 75.00 32.333 1.0000 22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10 137.00 75.00 32.333 1.0000 45.2 . 32;800 1.1007 32.800 30.000 36.000 5.142 10 144.00 75.00 32333 1.0000 72.6 33.100 1.1309 33.700 30.000. .38.000 7.001 10 147.00 75.00 32.333 1.0000 100 35.900 1.2047 35.900 32.000, 38.000' 5.326 10 153.50 75.00 32.333 1.0000 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01). 1.045023799 1.035 0.04119118 .0.090545302 Bartlett's Test indicates equal variances (p = 0.46) 4.634984493 15.08627224 The control means are not signifieantly different (p =0.73) 0.350059429 2.100922029 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steels Many-One Rank Test t00 >100 1 Treatments vs Control-I Linear Interpolation (200 Resamples)
Point % SD 95% CL Skew IC05 >100 ICIO >100 ICI 5 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqn 101_11-17-09data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Statistical Analyses
, Environmental Testing Solutions, Inc.
Used for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/17/2009 Test ID: CdFRCR Sample ID:- TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 TestSpecies: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10..:.
Control-I 32.000 29.000 26.000 30.000 27.000 30.000 28.000 29.000 .31.000 .33. 000 "
Control-2 31.000 27.000 30.000 32.000 28.000 30.000 31.000 30.000 31.000 28.000 11.3 32.000 31.000 28.000 31.000 30.000 30.000. 30.000 28.000 29.000 . 33.000 22.6 32.000 32.000 29.000 32.000 32.000 32.000 32.000 32.000 32.000 34.000 45.2 33.000 34.000 33.000' 31.000 32.000 36.000 30.000 34.000 33.000 .32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30000 32.000 100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000 Intake 38.000 35.000 33.000 30.000 29.000 33.000 32.000 .31.000 31.000 33.000 Transform: Untransformed I-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 29.500 0.9899 29.500 26.000 33.000 7.366 10 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000': 5.362 10. -0.840 2.287 1.906.
22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10 -2.879 2.287 1.906 45.2 32.800 1.1007, 32.800 30.000 36.000 5.142 10 -3.959 2.287 1.906 72.6 33.700 1.1309 33.700 30.000 38.000 7.001 10 -5.039 2.287 1.906 100 35.900 .1.2047 35.900 32.000 38.000 5.326 10 -7.678 2.287 1.906 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <0 0.01) 1.045023799 1.035 0.04119118 0.090545302 Bartlett's Test indicates equal variances (p= 0.46) 4.634984493. 15.08627224 The control means are not significantly different (p = 0.73) 0.350059429 2.100922029 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 1.906063607 0.064612326 55.14666667 3.474074074 1.3E-09 5,54 Treatments vs Control-I sqnlO _1-1i-7-09data
io TVA I Sequoyah Nuclear Plant, Outfall.101 - Intake Non-treated November 17-24, 2009.
Statistical Analyses Environmental Testing Solutions, Inc.
Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/17/2009 Test ID: CdFRCR Sample ID; TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia.
Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 .
Control- I 32.000 -29.000 26.000 30.000 27.000 30.000 28.000 29.000 31.000 . 33.000 Control-2 31.000 27.000 30.000 32.000 28.000 30.000 ,, 31.000 30.000 31.000 ,28.000 11.3 32.000 31.000 28.000 31.000 30.000 30.000 30.000 28.000 29.000 33.000 22.6 32.000 32.000 29.000 32.000 .32.000 32.000 32.000 32.000 32.000 '34.000 45.2 33.000 34.000 33.000 31.000 32.000 36.000 30.000 34.000 33.000 32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30.000 32.000 ,
100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000 Intake 38.000 35.000 33.000 30.000 29.000 33.000 32.000 31.000 31.000 33.000 Transform: Untransformed .- Tailed" Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD .
Control-I 29;500 0.9899 29.500 26.000 33.000, 7.366 10 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000 5.362 10 22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10 45.2 32.800 1.1007 32.800 30.000 36.000 5.142 10 72.6 33.700 1.1309 33.700 30.000 38.000 7.001 10 100 35.900 1.2047 35.900 32.000 38.000 5.326 10 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10 -2.793 1.734 1.676 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wiik's Test indicates normal distribution (p > 0.01) 0.951577842 0.868 0.62319944 1.12773217 F-Test indicates equal variances (p = 0.18) 2.563559294 6.541089535 The control means are not significantly different (p = 0.73) 0.350059429 2.100922029 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.676261504 0.056250386 36.45: 4.672222222 0.0120129 1, 18 Treatments vs Control-2 sqn101_11-1 7-09data
TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated November 17-27, 2009
- 1 - Ceriodaphniadubia Chronic Whole Effluent Toxicity Test m tEPA-821-R-02-013, Method 1002.0 LKS Daily Chemical Analyses Environmental Testing Solutions, Inc. -Project number: 5812 Reviewed by:
Concentration Parameter
________ _______________ initial Final Initial I Final Initial I Final I Initial Fnl Initial Fnl Initial I Final I Initial Final PH (SU) 7.48 7.58 7.631 754 7.501 7.711 7.71 78 7.55 7.5 -*7.421 7.571 7.79 7.51 DO (mg/L) 7.8 7.8 7.61 77 7.71 8.01 .7.8. 8 799 8. 7.81 8.01 8.0 7.8 Conductivity (I~mhos/cm)3232M36341111111111
- Control Alkalinity (mgIL CaCO 3)612=2 2
- Hardness (mg/L CaCti))
______Temperature (C) 24.9 24.9 24.9 24.9 24.8 25.11 24.6 249.27 2. 24.71 24.91 24.81 25.3 pH SU) .. 7.79 7.57 7.66 7.56 7.57 7.711 7.73 768 .68 75 7.571 7.58 7.621 7.72 1.%DO (mWL) 8.0 77 7.7 76 7.7 8.1 8.2 81.81 78.01 1 8.0 7.8 1.%-Conductivityý(pnhos/cm) 317__ 7_1330_37_0329
_T___
______Temperature CC) 24.9 24ý9 24.9 25.0 24.9 25.1 24.9 29 24.7 24.7
-50 25.2 24ý9 25.1 pH(SU) 7.74 7.57 7.62 7.55 7.56 7.71 7.72 78 7.65 7.4 7.53 7 59 7.61 7.52 DO (mgIL) 8.0 7.7 7.8 7.51 7.9 8.1 8.2 8. 8.0 8.0 7.9 81 8.1 7.8 22.6% !Conductivity(pMmbos/cm) 2992326 2826 .286 280
______Temperature (1C) 25.0 25.0 24.9 25.0 24.9 25.1 24.9 20 24.7 250 24.7 25.0 24.9 25.0_
OH I!U1J! 1./Al ~f.~6I ~I.6II 7541 7.511 7711 7711 7661 7671 75tI 1471 ~7tRI iWI *7~I 7.73/L 7.5 7 M8.~ 75__51_71 77_6_77 __ _ 75 79 5 DO ___________ i ______ 81 781 . . 7.91 .111 81 7.9 8.01 8.0 8.1 78 45.2%
Conductivity (Limbos/cm) ______5_29 ý 5425 ý Temperature f°C) .24.71 24.91 24.71 25.01 2491 25 2 6- 6. -
DH (SU) 6.-
7.70 7.56 7.591. 7.54 7.481 76 7.671 7.651 7.601 -2r_ 741 7~ 6 .5
_ .7,5674 72.6%
DO (I _/L)
Cnand~r tlviii /..hkn/rmI r 8.1 . .8 7.71' 7.6 7.9 80 8.21 8.21 8.31 7.91 8.21 8.01 8.2 7.
225 221 22 2171 2 23ý_
- 1. 21 0
Temperature ( C) 25.0 .24.8 24.91 24.9 25.0 . 48 .25.01 25.11 2' 4.81 24.91 24.81 25.11 ; 24.91 24.91 pjH (SU) 7.67 7.56 7.571 7.54 7.44 7.6 7631 7.62 7.
[DO (mg/L) 8.1 7.8 7.7 7.61 8. 8.0 8.0 . 8.2 Conductivity (Mahos/em) 181 1817 6 67 168 166 100% Alkalinity (mg/L CaCO 3 )
[Hardness (mg/L CaCO3)
Total Residual Chlorine (mt/L)
_ _ Temperature (*C) 19J 2481 2481 2511 2491 25 I___
oH iSUl 521 7.49[ 7.26 7.56 7.47 7 82 6 DO (mp/L)
Conductivity (Limbos/cm)
Alkalinity (mg/L taCti3) 24.91 24.8 24.9 25.011 25.01. 25.0 25.0 24.8 2' 100% Intake Hardness (mg/L CaCti,)
Total Residual Chlorine (mg/L) __
Temperature ('C) "
I *a
- 1onmNr~* omo*i Page 6 of 7 I Species: Ceriodadhniadubla- Date: . 1 -,,
I Client: TVA / Sequogah Nuclear Plant, Outfall 101 Daly hemstry:
Day I i I Concentration U
I Parameter Analvs t 1 Al AZI .1~
,pH (S.U.) ... '-
Conductivity (umhos/cm) 41 CONTROL Alkalinity (mg CaCO 3/L) -4 Hardness (Mg CaCO 3/L)
Temoerature (°CI
. PH (S.U.)
11.3% Conductivity, I *___-' _
(iimhos/crn)
Temperature (°C) 14A I
,6 I -, - II 7%1 I ".S*, 1I' +1 . II I 22.6% Conductivity (jimhos/cm)
T~F1-~AF~TF Temperature (°C) pH(S.U.)
ILC. 0
".** 1 1.S
I iW LL5~
45.2% Conductivity v4X I __________
Temhos/cm) 1 Temperature ('C) pH (S.U.)
A
-S.O
'4, -h0
,0(0 S
II 7.Sq I t.4PR
.. ai--
4 -'-~----4I----4. -,,,1. *. .
DO (mW,/L) 72.6% Conductivity- I
,.ýmhs/ctn 'LS Vk - 02 Temperature U U oH (S9.11
...... j 1 DO (minL/ ý1 c~I I Conductivity Ie Alkalinity 100%
-LM-9CaC0 3/L) ____
Hardness 1l0 (Mng CaCo 3 /L)
T!RKchlorine (mg/L) 01
- .S.0 U
Temperature (°C)
S~
is. 0 l*l " - J nH (S.U.) 1.L42, a -~--n-DO (mLY/L I Conductivity -72.
100% Alkalinity I Intake (Mg CaCO 3/L)
Hardness (mg CaCh 3 /L)(ra./L (A
Til chlorine (mgfLw 0 a
Temperature (QC) a "1.L. 9 - - -
n rIa. Fin Initial 0lal i~nitial Fi~nal Page 48 of 102 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09
r
- a 3
ss.um.A.tSJTbA*q 5* ..,%
Page 7 of 7 Species: Ceriodaphniadubia Client: TVA / Sequoiah Nuclear Plant, Outfall 101 Date: (I-1-00i
'I Dav I Concen- Parameter tration, -
i
-P SU)-,
DO (mnfL)"
_4-. J--I Conductivity (gmhos/cm) Iv CONTROL Alkalinity (mg CaCO3,L)
Hardness (mg CaCO3/L)
Temoerature (°C0 1_1 - ItAl _LS.0 -Lk. I Temperature ('Q nh-" (* ,~1 .*~ cvA Ii.aI 1 1 '- 4 1 -3Ii1- HIt 'T 1"4atZ H q DO (mg/L) '49 3 II - .: - - I-I f3, I I ýeýo 1114 41 11.3% Conductivity (jgmhos/cm) 301 o.3 Temperature (0C) .1 q p.
j DH (S.U.) 11 2- 1 ADS 3 1 DO (mg1L) 04 a. 9 A 0-1 22.6% Conductivity (gmhos/cm) 2-9 (P B (0 215 Temperature (QC) VAgk 1 0 -Ls. 0 1 1 -LS.0 ot -L%. C)
PH (S.U.) TU2 5 2,14-ý DO (mg/L) B.- 6A 1910 45.2% Conductivity jTemperature (Tamhos/cm) nHl (S.U.t (0 CQ IS-0 1j_(10q vk- 1A.1 I -too
-LAA
,'4 -sip I -Ls.-L-PH (S.a DO (mi_/L) e.z.
Conductivity 2_j (jumhos/crn Tempoerature (°C0 "L5. I i I -emperature -
PH (S.U.) %Q42- 1.55 q.ALaa '4, q' '104ta DO (mg/L) p -
Conductivity
('mhos/cm)
I 100%
Alkalinity Hardness '
(07-61 e(mrg CaCOtrL)
< 0,10 Temperature (°C) I I 1qA il MIA. -LA-f I _LS-1 p
DH (S.U.) I ~Iit~n3 I.2 I :ý1 I
-4, (po 1A[
DO (mgfL)
Conductivity (gmhos/cm) -7 100% Alkalinity Intake m CaC03/1, Hardness (mg CaCO3/L)
TR chlorine (mgfL) 1(e"I (0.10 Temperature ('C) Z5.0 tg. o -4. 0 7.,j 1 17Z.q Ls.
Initial Final I Initial Final Initial Final F: Initial Fin Page 49 of 102 P SOP AT I1- Exhibit AT 11.2, revision 04-01-09
S Page I of 6 0
9 ETSTl
. Chronic 6 -Whole Effluent Toxicity Test (EPA-821-R-02-013 Metiod 1000.0)
, ... Species: Pimephalespromelas .
I: Client: TennesseeNalley Facility: SequoyaNe NP DES#:
uthority Plat -.
County:
Oufall:101
,,O,.X_._.....L.,,
d 1AMHILro'..J I Projec# 5&Vý Dilution preparationinformation: Comments:
Dilution prep (%) 1.3 22.6 '45.2 72.6 100 Each concentration was UV-treated I Effluent volume (mL)
FDiluent Volume (mE) 282.5 2217.5 565 1935 1130 "1370 1815 685 2500 for 2 minutes to remove pathogenic 0 -1Interferences.
Total volume (mL) 2500 2500 2500 2500 2500 I Test organisminformation: Test information:
Organism age: 1-1.So -0A * )Vt.. " Randomizing template: ,
I Date and times organisms were born between:
Organism source:
- t Incubator number and shelf location:
Artemia CHM number:
j(AR4%%
I Transfer bowl information: pH = *.6O S.U.
Drying informationfor weight determination:
Date / Time in oven: -0 I 'Average transfer volume: "
Temperature =q.b1°C Initial oven temperature:
Date / Time out of oven:
Final oven temrerature:
1!-*
l C..
(o0.
1360 I __._,,,__Total Dailyfeeding and renewal.information:
drying time: -,oj.
Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch
__ _ termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake
- 0 60 A____ 30o it 144001
~ O~1O'S L -. o f~.O
___ 1-OV
______ R-o A0 1oO b 1,62 o 01 It Vj-DS 0111ufLo( 1 I'mA 11-17.-M I -3 ' 11 "?-A- Oac No t, .
A 1, o l t qbt Pi a 4
,S m
IZ.
-'" s ' IaI I.
ocit*," a I& . -.'Ot I %oaw 01l I It.11*-f 4
o2-6oci Ims%-i.-~ IA N 7
-it- zq. 41 L3's Controlinformation: Acceptance criteria Summary of test endpoints:
% MortalitTy. I . <20% . 7-day LCo )_1007..
Average weight per initial larvae: .
862. NOEC 10.,
Average weight per surviving larvae: 0.901- "0.25mg/larvae LOEC iO,>7.
ChV > 1001.
IC25 - [ 10047.)
SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 50 of 102
Page 2 of 6 Suinsi Species: Pimephales r6ielas..
Client: `TVA 1TSeaUovyah-Nuclear Plant. Outfall 101Uv-itreated Date:a I- I"1*0' :.!?
- 9
f ;-
-~ Survivaldand.Growth Data j.*.:.,*
Daya . -CONTROL' :1. 3% - -. 22.6%
. - V-treated A B C D E F G H I J K L to o0 to /0 1 ) tO 0 /0 /(00 /0 /0 2 j I to /oI1 to /a0 /0 Gl0' .tO to 0 20 / tOI 10 0 1 10 1o t 10 o to
/4 t 100 IO 10 10 /0 1/0 /0 /0 60 10
____________ If I(0 /,) /0 1(0 /0 0 / 10.0 /6 1 1
I6 1I010 . 1 I0 94 Ioi0 j.O /0 -io
- 6. /0~ 10 1 1c101c O go 10 /0 .O /0 to A = Pan weight (mg)
Tray color code::
Analyst: Y*-* . 1' I 1.,-* t I't.7
.# 1'0 4":T- 1'5 IS,7o H9.qI J-5.-t3 1i'/*5" 1146).1* b Date: 10-?-," -tq-i B = Pan + Larvae weight (rag).
Analyst: fýkS 2Z.,',O aIS L..- o 22.7 t...Z.*J2 . Zt. ?..Z . 3 2,A- Zl. -Z-Z.VI 2L. 02%A.4 2
Date: w.ight (Om)
C = Larvae weight (mg)
=B-A gq ,* "11.1I ,*$1 .) ,, )* i*)8S ,(
Weight per initial number of larvae (mg) %0 P., 9.4
= C / Initial number of as larvae
'I 16 0 V' Cs 1
Average Percent weight per *reduction o~~g~s (.got .7 initial from .81.
number of j control (%)
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually.large, d&r = decanted and returned, w = wounded.
IComments.* Calculations and data reviewed:
Page 51 of 102 SOP AT20 - Exhibit AT2O.3, revision 04-01-09
I, Page 3 of 6 D)ETS-I SEnvironmnwrtal TestingSouinInc r Species: Pimephalespromelas.
Client: TVA / Sequoyah Nuclear Plant. Outfall 101. UV-treated Da te: I%-i I-ai s II _ _ M Day"*.45&.2%
N, 0 p~O
.Suvivaland Growth
- -*72.6%
ata T -R U V
100%
W X (0 o iO I 1 /0 to /o I0 /0o /o 0o /0 1 (0 tO 10 /0 to /0 1 /0 /0 0 0 /
2 10 to /Q to .16 to t , t10 /0 10 /0 to 3 1IU. /0 I10 /0 ID 0/C /0 /0 11D 0I 4_ 1 / 10 to (0 //0 I 1 0 1 .16 t0o 0 I
_./D 5
__ /0 10o 0 10 1o I i i 10 /0 10 10 (0 6 10 o t0 1 /b 0 / 6 O 0 .( 0 /0 I 7
/10 1lM0 10 10, 10 /0 10 /0 10
%-U_
1tD A = Pan weight (mg)
Tray color code::
Analyst: . Y- I38.,;-7 14.91;? 1 L335' 1.547 j'T7 ,-5
. 'ot7 q l- .
/q.",i *"t Date: 1D - *'i -Q*1 B = Pan + Larvae weight (mg)
Analyst: 7 \.43 2 l'l Z.Oi Z4-.S V7.72- Z3,('4 \.Zt7 O.C3 zz.12 2 ,*C .
Date: *" *\:0 C = Larvae weight (mg) oB-A ( 811 &%tS &J0S
- j.i S.S , 1.16 ltS 0 %..
13 Weight per initial number of larvae (nig) oflra'4
=C / Initial number of larvae *9*
5~~
II Average weight per initial Percent reduction from control 07' 00'. g*5' -q.{i.
number of Ii (M-larvae. )raI Comment codes: c = clear, d = dead, fg = fungus, k= killed, m = missing, sk = sick, sm = unusually small, I] lg = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:4\
II Comments:
Page 52 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09
I Page 4 of 6 ETS 0
S 0
9 1 D Envfr)Iuonmr ht nct.l
... Species: .Pimebhalespromelas Cl.,.,Client: -TVA-/ISeauoyah Nuclear Plant, Outfall. 101E. Vtreated 'Date: [I-11-0*L.
- SurialandGr6wth.-b Day 100% Intake Y
__ Z AA BB 0
/0 tO 2 10'0 10 o
.3 b~ '0 to M
- 4 i 5 5 1 0 10 D 1D 10 /0 /0 7
A = Pan weight (mg)
Tray color code:: /
Analyst: IS , IS-773 1 13..
Date: jo- 1-0-g B = Pan + Larvae weight (mg)
Analyst: __.,A.5 2-3,53 ZOAIO Date: ' '1-,-'- I 0c C = Larvae weight (nag)
Weight per initial number of larvae (mg) %, C N
= C / Initial number of larvae Average Percent weight per reduction Initial from control number of (%)
larvae (rg)I .*
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed: -
IComments:
SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 53 of 102
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 17-24, 2009 PimephalespromelasChronic Whole Effluent Toxicity.Test EPA-821-R-02-013, Method 1000.0 -n il, Quality Control Environmental Testing Solutions, Inc. Verification of Data Entry, Calculations, and Statistical Analyses Project nmber; J." 5812
+
._______ Not for Comptliance Assesatoerst, MLaboranto7y eQCvei" edb.'_
Colee fli*sa %) Ropldku talt l i .. amrdf laat-rFito .. mi ber hrv. A-Pa w 0t
(.1)
B=P. Lt
-dtt (.t0 Larva ,w19 L-s-+Larvae
-A-9
(.1 ) WeightISievavi.d hu-e naiuherof Me (ait) SUarni amhuerot Catff el Of -
/.eghtI I- '~
- laamWev in o
of i .1-1W.&Mer h Welghsit i O l rMeri 1%) 1.4W l...b.
1,dghllt w
if 4amhor Cad ~
.O oi.
of Petare d.t
-atrd(%)
fe-laati1.
fes 1-m, (0g) hl-. -l)(%) ; ` ,,l- ve (a.9 .. -.
A 10 10 14.32 22.80 8.48 0.848 0848
,.Control 8 C 10 10
-10 14.97 12.74 22.15 20,46 .7 7.18 7.72 072 0.718 0.772 0802 01287077 8..770.6 .72100.0 07 00781 '07 - + "0.802+ . 8.7
. Not applicable D 10 10 14 .1 1 2 2 .8 1 8 .70 0 .87 0 0 .87 0 . ... . . .
E 10 10 14.06 22.82 8.76 0.876 0.876 F 10 to0 14.05 22.62 8.57 0.87 0+834 650.857 9.. 0 . 77 . -1.4
,G l0 9 15.70 23.34 7.64 0.849 0.764 ,
H I0 10 14.43 21.98 7.55 0.755 0.753 ' -.
I to 0 13.63 21.12 7.490 0.749 22.6% 10 10 14.15 22.11 7.96 0.796 54 0.796 -00.0
, ,' o.os 54 0.1 K 10 10 12.46 21.00 8.54 0.854 . 0.854 L 10 10 13.82 21.88 8.06 00.806 0+806 M 10 10 13.84 21.08 7.24 0.724 *0.724 10 13.27 21.43 8.16 0.816 0808 7.4.86 100.0 45..% O23.17 8.25 0,825 0.825 0.808 7.4 -0.7 "P 10 0 13.36 22.01 8.63 0.865 . 0,865 ""_+' .' " "
Q 10 10 13.35 22.25 8.9+0 o890 0o890 72.6% R .10 10 13.47 21122 7.75 0.775 0134 6.0 0.775 O00.67 0.834 .6.0 -4.0 S 10 £o 14.87 23.43 856 0.856 0,856 100.0,.0 T 10 £0 13.07 21.21 8.14 0.814 0.814 ________J___
U 10 10 13.18 20.93 7.75 0.775 0.775 100% V 10 10 14.61 22.18 7.57 0.757 01835 10.1 0.757 100.06 ' 0.835 10.1 -4.1 W 10 10 14.66 24.05 9.39 0.939 0939 ý ; ""
X 10 10 15.39 24.06 8.67 0.867 U0.867 -,
Y '0 10 15.40 23.53 8.13 0.813 - 0813 100% Intake
- Z 10 10 £3.16 20.42 7.26 0726 0.772 4+6 0726 100.0 0.772 4.6 . 3.7
" AA 10 10 15.73 1 23.53 7.80 0,780 . 0.780 Ia 10 10 12.86 20.56 7.70 0.770 0770 ' ,
outfall 10£; MSD Mmnimun Significant Differen*e Dunnelt's MSD value: 0.1075 PMSD = Percent Minimum Significant Difference PMSD: 13.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statiscally significant u a wholeeffluent toxicity test Intake:
Dunnett'a MSD value: 0.0764 Lower PMSD bound determined by USEPA (10th percentile) : 12%.
PMSD: I
- 9.5 Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET nhirlabofatoryVariability Sthdy (USEPA, 2001 a; USEPA, 2001b).
USEPA. 2001a 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluest Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0I-004 arid EPA-821-B-0I-00S. US Environmental Protection Agency, Cinctnrats. OH.
TVA /Sequoyah Nuclear Plant, Outfall 101 UV-treated "S
0 November 17-24, 2009 Statistical Analyses
!,* Environlmental Testing Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/17/2009 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear: Plant,, Outfall 101 End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dischargc Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.8480 0.7180 0.7720 0.8700 11.3 0.8760 0.8570 0.7640 0.7550 22.6 0,7490 0.7960 0.8540 0.8060 45.2 0.7240 0.8160 0.8250 0.8650 72.6 0.8900 0.7750 0.8560 0.8140 100 0,7750 0.7570 0.9390 0.8670 Intake 0.8130 0.7260 0.7800 0.7700 Transform: Untransformed I-Tailed Isotonic Conc-% Mean N-Mean Mean Mm Max CV% N t-Star Critical MSD Mean N-Mean D-Control 0.8020 1.0000 0.8020 0.7180 0.8700 8.727 4 " .. , . 0.8153 1.0000 11.3 0.81,30 1.0137 0.8130 0.7550 0.8760 7.672 4 -0.247 2.410 ` :1O75 0.8153 1.0000 22.6 0.8013 0.9991 0.8013 0.7490 0.8540 5.374 4 0.017 2.410 '0.1075. " 0.8153" 1.0000 45.2 0.8075 1.0069 0.8075 0.7240 0.8650 7.381 4 -0.123 2.410 40.1075 0.8153 1.0000 72.6 0.8338 1.0396 0.8338 0.7750 0.8900 5.997 4 -0.712 2.410 0.1075 0.8153 1.0000 100 0.8345 1.0405 0.8345 0.7570 0.9390 10.150 4 -0.729 2.410 "!0.1075 0.8153 1.0000 Intake 0.7723 0.9629 0.7723 0.7260 0.8130 4.648 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.939999402 0.884 . -0.02446093 -1.22636643 Bartlett's Test indicates equal variances (p 0.91) 1.50255847 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 .0.107485809 0.134022205 0.000919567 0.003978306 0.943894863 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point % SD 95% CL(Exp) Skew IC05 >100 ICIO >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100 sqnJOJJJ-1 7-O9data-uv
TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated November 17-24, 2009 Statistical Analyses Environmental Testlng Solutions, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: .11117/2009 Test ID: PpFRCR Sample ID- TVA / Sequoyah Nucleai Plant, Outfall 101 - Intake End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas .
Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.8480 0.7180 0.7720 0.8700 11.3 0.8760 0.8570 0.7640 0.7550 22.6 0.7490 0.7960 0.8540 0.8060 45.2 0.7240 0.8160 0.8250 0.8650 72.6 0.8900 0.7750 0.8560 0.8140 100 0.7750 0.7570 0.9390 0.8670 Iniake 0.8130 0.7260 0.7800 0.7700 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.8020 1.0000 0.8020 0.7180 0.8700 8.727 4 11.3 0.8130 1.0137 0.8130 0.7550 0.8760 7.672 4 22.6 0.8013 0.9991 0.8013 0.7490 0.8540 5.374 4 45.2 0.8075 1.0069 0.8075 0.7240 0.8650 7.381 4 72.6 0.8338 1.0396 0.8338 0.7750 0.8900 5.997 4 100 0.8345 1.0405 0:8345 0.7570 0.9390 . 10.150 4 Intake 0.7723 0.9629 0.7723 0.7260 0.8130 4.648 4 0.756 1.943 .0764 Auxiliary Tests Statistic . Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96844363 0.749 -0.33306569 -0.81375687 F-Test indicates equal variances (p = 0.30). 3.80257463 47.46722794 Hypothesis Test (1-tail, 0.05) MSDu MSDp . MSB MSE F-Prob . df Homoscedastic t Test indicates no significant differences 0.07642232 0.09528968 0.00.1770125 0.003093458 0.478019476 .1, 6 Treatments vs D-Control sqnJO1_l M-7-O9data-uv
........ .......I....
TVA/ Sequoyah Nuclear Plant, Outfall 101 - UV-treated November 17-24,2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Li Daily Chemical Analyses Environmental Testing Solutions, Inc. r.Project number: 5812 Reviewed by: f L Concentration Parameter Conductivity (tunhos/cm) 332 Co0trol Alkalinity'(mg/L CaCO 3 )
Hardness. (mg/L CaCO3 )
- Tern rature C 25.0 24.8 24.9 4. 24.7 24.9 24.8 24.7 24.8 24.8 24.8 24.7 24.7 pH (SU) 764 7.63 7.59 7.39 7.68 7.51 7.63 9 7.18
__DO
_m__/L) 77 76 8.0 3 7.9 7.4 0.8 779 70 11.3% Conductivity (jumhos/cm) 3100100
.... Temperature CC) 25.1 24.6 24.9 9 24.9 25.0 25.0 24.8 24.8 2468 248 24.9 24.7 pH _____(SU)_____ 7.63 7.49 7.63H 7*.40O 7.57 7.38 7.69 7.52 7.62 7 53
.1 7.18 7.60 7.18
- DO (mg/L)___ (pmhos/cm) 7.8 7.6 8.01 7.31 7.9 7.1 8.0 7.7 7.9 72.9 7.1 8.1 7.1 22.6% Conductivity 299 296 293 2 92 292290_9 Ternerature CC) 25.1 24.6 25.0 24.9 24.9 24.8 25.0 24.8 24.8 2468 24.8 24.9 24.7 pH (SU) _______ 7.61 7.49 7.62 7.37 7.53 7.36 7.68 7.481 7.60 7T6 74 71 7.59 7.15 BO (rag/L) 79 75 80 72 79 7.1 8. 77 79 739 7 8. 70 45.2% Conductivity (junhos/cm) 26__ 576 TemperatureCC) 252 247 250 24.8 25.0 8 25.0 248 8 2424 24.8 247 249 247 pH ______U)___ 7.59 7.47 7.62 7.35 7.491 7.34. 7.65 7.48 7.53 71. 43 719.57 7.15 DO ____________ 8.0 7.4 8.0 7.2 801 7.31 8.1 7.7 7.8 738.0 6.8 8.1 6.9 72.6% Conductivity (iemhos/cm) _20 223 2417171 Temperature C .252 247 250 24.8 250 250 250 246 248 248 250 246 250 249 PH (SU) .7.561 7.441 7.57 7.38 7.4 7.301 7.621 7.47 7.511 7.091 7.37 7.16 7.521 7.131 DO____________ 8.01 7.61 8.01 7.3 8.01 7.11 8.11 7.8 8.0 7.3 8.0 6.9 8.21 6.91 Conductivity (junhos/cm) 100% Alkalinity (mg/L CaCO )
3 Hardness (mg/L CaCO 3)
Temperature CC) 25 3 24:7 25.2 24.8 25.0 24.8 25.2 24.7 249 247 250 2491 25.1 24.7 DO______________ 8.31 7.71. 8.01 7.3. 8.11 7.31 8.21 7.8 8,0 7.2 8.21 6.91 8.21 6.9 Conductivity (punhos/cm) 1 100% Intake Alkalinity (mg/L CaCO 3)
Hardness (mg/L CaCO3 )
Temperature C) 25.2 24.5 25.0 24.8 25.0 24.8 25.1 24.6 25,.1 24.71 24.91 24.6 24.9 24.8
Page 5 of 6 Cm~~mnmenta luTdinofues Inc.
Species: Pimephales promelas Date:
Client: TVA/ Sequoyah Ni leair Plant. Outfall 101, UV-treated Dally Chemistry:
Ifays .11* I LA ) ,:,
Concentration Parameter.
Conductivity 53o)
CONTROL (Amhos/cm)
UV-treated Alkalinity H(mg CaCr 3/e)s Hardness (mg CaC0 3/L) g
____ ____ Temperature ('C) -LSo 14.1i~. ~ .
nfl( {ST I. 74.Uq~ 11 'JMM 41 lA.~
PH (SU) a I11 2 .. M DO (mg/L) .0 11.3% Conductivity 1 ~lO1 (P mos/cm) -. 1~ Si2t Temperature (°C) Lii. I '-'IA 4 'LIS -
PH (S.U.) 7-S7
.22.6%
DO (mg1L14 A Conductivity
-00 -I.C)
(iAmhos/cm) 0100 201 L.0 Vq. *3 Temperature ('C) 1S - 1 1610 A 0
nH (S.U.) :9,40 1 14,(02-4-
DO (mWL) '42 45.2% Conductivity (A+/-rnh s/cm) 1 A(44 Temperature (°C) 1S. VA-1 15.0 0 a -
pH (S.U.) q.(07- t4cl DO (m/-L) E). C) 72.6% Conductivity BIB mgrhos/cm) U3 Temnerature (MCY 'LIS .0
p-s.U.) H I.qq 80 8. o Conductivity .-
(tgmhos/cm) - 101 lot 171 Alkalinity 100% (Mg CBCO 3 68 Hardness I _
fb'WIt*ftfb (mg CaCO 3/L)-
Temperature (C 4 0.1 TS - Pý ý[
I DH (S.U.)
DO (mgWL)
Conductivity /~
1 II 4,qq 1 14. kn 111.151 I I.qq
.1 11 74 II I .
" Intake k100% Alkalinity (mg CaCO 3/L)
Hardness (mg CaCO3IL)
Temoerature (. C) iTemperatur I -(
1 "S.2.-
Initial ii m b.U Initial ii II .,&
Final 0.10 Initial H.'
I L'4*
Final ]
I I Page 58 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09
r Page 6 of 6
~)Env~roi~mgnt IuTfngShlon.,
Inc.
.Species: Pimephalespromelas Client: TVA./ Seau6vah Nucleai'Plant. Outfall 101., UV-treated Dae: iI--
Dav I 6
An'Aalyst L Concen- Parameter,--
pH (S.U.)
DO (mgh/L)
Conductivity CONTROL (Mmhos/cm)
UV-treated Alkalinity (Mg CaCO 3/L)
Hardness (me CaCO,/U*
(Mg Ca03/L, Temperature 4 _LIM (MC)
- , _ l- ,
pH (S.U.) i~1 1-DO (mg1L) 11.3%
I(gtmhos/cm)
Conductivity Temnerature ('CQ 4,U v .Ig 1A-A -A -
-V I 22.6%
pH (S.U.)
DO (mg/L)
Conductivity
~LO Df .
'-t~ I .C57 1 7.1n2. 11-,~.-.T(
33 I 1.V?
rýIq I-
' I _4tn 'II0 ._1~
Temperature (;C) `10 U P ~
PH (S.U.)
45.2%
DO (mg/L)
Conductivity (grnhos/cm)
Temperature (0C)
I L~~-O i7.
':~.,5-I.5 L a~ 0)
I i'{~
II4L)1 4
I 4-~ I'% ~ 65'7
-. P.
oH (S.U.)
ý 2ý DO (mi/L) a~ll1B .4-3 1 72.6% Conductivity (gmhos/cm)
Temperature ( 0C) '-Ms0 VUI -LA. a _QL. -M n -LA. %0 -Ls. n i%1.1 I
..... i 0.11MMIN111111M pH1 (S.U.)
P /T -t 1.41i I .,l Ii-c ;.(o I " ,2. II H ,1. I DO (mg/L) 8.0 1 Conductivity.
(gmhios/cm) ILD~
Alkalinity
- 100%
(Mg CaCO3 /L)
Hardness (Mg CaC0 3/L)
AM-A'eMPb- TR Chlorine (mg/L) 40A. to Temperature (°C) 1. -L. 2- ilq --I.,
pH (S.U.) "7.cG
- - I:
II DO (mg/L)
Conductivity
-(Amhos/cm) 8o' 100%,.. Alkalinity Intake (mg CaCO 3/L)
Hardness IOaO
'(Mg CaCO 3/L)
Temperature (0C) -14C. II- II (.4. (a
-M -
II Initial II Final m II Initial ll II Page 59 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09
Page 3 Page__tof I 0
. EnvlronmentalTestlng Solutions, Inc.
Total Residual Chlio'en.ne (Orion Eletrode Method, Orion 97-70)
Matrix: Wateir . = lO g ".
Meter:'Accumnet Model AR25-pH/1-on Meter.
Date aaye cdegn Calibration:
0.10 mag/L 1.00 must a Slope e Reference standard number I qt, "15S JS Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV X 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)
I N5SL&? 0.50 Ov4 2..
Duplicatesamp"le precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD {(S - D) /[(S+D)/2})x:100 nube (al)(acceptable range = 10%)
01)"11-1. OA btA~icu l-c-CF Llm~xrt. color S0.31 Duplicate D o.oIq0 Sample measurements:'
Sample Sample ID Sample characteristics Residual chlorine number m b Reagent Blank LOOoBL, o0%1111. Jtb' IN- Wj Clef -NO C0 I QV; f PIo.41-el8 60,111-1-0 . dcovJSO paj(+) r3 0 .0W 'L~OL6fl O'M16 .02. V n F-1Z*4T -IA\iaclor cak a .. t-o0. 0040 3 Oct IN, -. 1 'nT-W DSN1\J-1k 001- 1; Sigittycowij I pa It-4-aN OO25 I-. J "=.0 1U GOW1thi.S S, lý-( ~ ~ ci/~Caliaes lcak +ONi O0o002-00 M ilti0.6 %N T0-/' _._-_10: 0.0011_7 Znrt__.-
Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratorycontrolstandard:.
Reference standard True value (TV) Measured value (MV) % RS = MV /TV x 100 number " (mg/L) (mg/L) (acceptable range =90 to 110%)
Z445 61 0.50 0. LAI1 C)- 4J Reviewed by I Date reviewed _ 1.-1 jJ Page 60 of 102 SOP C8 - Exhibit C8. 1, revision 09-01-09
Page 5 I Page __ of L.
F.nvlronmentalTestlng Solutions, Inc.
Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: .Water,RL-= 0.10 mg/L Meter: Accumet Model AR25-pH/10n Meter t Aidýreagent:
fos ýQýj Date~analyipd: odde A rgnt:Tg.3 Calibration:
0.10 mg/L, 1.00 mu/L b Slope l Reference standard number 3 .I Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Lahnroinru innfrni.eiandard~
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rag/L) (mg/L) (acceptable range = 90 to 110%)
- *-*0l 0.50 o. I02..0"I.
Duplicatesample precision:
Sample, Sample ID Sample characteristics Residual chlorine ' %RPD = {(S - D) /[(S+D)/21) x 100 number.. .... numbe - S (mL) .(acceptable range =*10%)10%)2 Dupicato.oo1e D Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine numberb e Reagent Blank L0.OVf o)e Il:" ',hmr\gtoN V*XTQ , Lo .w32.
ocuL 5 , t35V ,- -IOV-T0oI_[x,*oocoaor
/,-e// . ,.oloO5 OrI16,0' 5*0*;A N-i .TO*, uckCoiteoir ,oc tJ ,ooqic
,,x itit CX31 C1 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratoriy controlstandard.
Reference standard number tr-.rs' Q13
[ True value (TV)
(mg/L) 0.50
-Measured value (MV)
(mg/L)
% RS = MV / TV x 100 (acceptable range = 90 to 110%)
100.14 Reviewed by I -3.
I.So O*
Date reviewed _11.
Iq.
Page 61 of 102 SOP C8 - Exhibit C8. i, revision 09-01-09
Page__7 SS Page I ofj
)
ETS--
Environmental Testing Solutions, Inc-Total Residual Chlorine (Orion Electrode Method, Orion 97-70)
Matrix: Water,.R = 0. 10 mg/L
- .. : l*):.,maiys:[.::L~
Meter: Accumet Model'AR25 pH/Ion Meter
- " ] '
- , - : ' ":-.' ': *' ' ',::Iodide de r rdagent:-t.
l.
.I, ,
[ .
-ADate an dcid reagent.: 1'=N2 .
Calibration:
Reference standard number of > 10ra/L h.00 r ge S lop e Note: Far samples with a residual chlorine of > 1.0 mg/L. the-calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L). (mg/L) (acceptable range= 90 to 110%)
- _,Ss~rI 3 0.50 - *o. l Duplicate'sampleprecision:
Sample., Sample ED Sample characteristics Residual chlorine [(S -
=RPD D) /[(S+D)/21) x 100 number (m (accetabl range =L,10%o)
- _ _ Duplicate~ _ _ _ _ __3CP Sample measurements:
Sample .. Sample ID Sample characteristics Residual chlorine number (mglL)
Reagent Blank O .
M Im1. 0~ 5W&S, cP\IO jCqAOc ~ ________
noAt JV N CAC II pck1d tc,.oooEoq CA (M L1+- . _-X -Ts4rerCim -n " tau
.d Ift 0'iii. T'P-RW-r- C. yr'~ wbputiduec. p k +cirN 000213 0iwIm 06 f tAFrrv- Cot C. 1&o+Or,4 Laig 082, 05 1 IzLo !. cS 5x- Jý Zk~ b__________ e.M))o pO0lo-OA' Samt.- Io(-Tcl*' A clI-eO.xv moz COIoy .. CLI Nrote: All samples were analyzed in excess of EPA recoimmended holding time (15 minutes) unless otherwise noted.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) %RS MVITV x 100
- number I(ung/L) I(mg/L) I(acceptable range = 90 to 110%)I qgýL J'LSýAm0.50. 0.4796 Reviewed by Date reviewed I LI 0 Page 62 of 102 SOP C8 - Exhibit C8. 1, revision 09-01-09
Page l(p Page ' of_-
- EnvftoiMlUtIalTIuing SolutIonh. Inc.
Alkalinity (SM 2320 B).
Matrix-.Water, RL = 1.0 mg CaCO 31L Analyst Time initiated 06&-'L Date.analyzed . ( Titrate samples to Time completed ONj .
pPH = 4.50 S.U.;.
Titrant normalitw'andmultlilier determinj pHo .of Normality *. Normality (N) of H0S" ' pH Factor or Multiplier.C-Deionized Titrant check Begin End Total = (5 ml NazCO3 x 0.05)/E = (N x 50000)/100 ml sample water "reference standard ml ml ml `- 0.25/E =N x 500 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220)
-4 .5 S.U. nu ~be nu mber13 0.
-7__ .f L Zo -
on o caND ~nitajrd; Reference standard True value Sample. Alkalinity (MV) % RS = MV / TV x 100 number . (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (Ing CaCO3/L) (ml) ml ml ml 90 to 110%)
1"00.1 100 Oz.o 2.'s C1.5" iO.b qo clO'.
Duplicatesam le precision: __"
T Sample - Alkalinity %RPD Sample . Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /j(S+D)/211 x 100
..- (acceptable range =.:k10%)
number tll-ar,* 0a%1zLOtD , AS (ml)
- ml A.4ml ml 61' 10.,4 s Duplicate (B) LgL.q 35.3T59 4 0 D
Matrix spike recovery:
Reference standard Spike'value Sample -.
- Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3 /L)
(mg CaCO3/L) (ml ml m ml
-00 36 I.0o0.4 110 Sample alkalinity (B) Measured spike value (MV) % .R = MV / SV x 100 (g CaCO 3/L) MV = A - B (acceptable range, (mg CaCO3/L= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml ml -i ml Multiplier. (mg CaCO 3/L)
I-t-1Lt\A8 NKS -o 0 310.1 4 4 .2' U.0 LOA (a?- __
11.IL1o0 4 0,o0 5 .5 to 11-09-0,9 '5-b -StO .0*31.4 46A 10-3.I10 31 0 i* it rL ..tas' P?- TP " ... q .5 ,C1.5 o .2. 2 ,1t n, It 1% -of _ p.a*-a., 60 10.*,*t.6' (2) 2.70 ci,,t o.o-- Fb,,ý.* wj - 110o A0o x-4 :AP' ae A.-t0- -1~
Reviewed by: I Date reviewed: -1$*8'-
Page 63 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09
I . Page R_
i Page 2- of *3 ivmnmental TesdngSoutia , ine, Jkalinity (SM 2320 B)
Matriý : Water, RL = 1.0 mg CaCO 31L Analyst 1i:v Time initiated Date analyzed .I .i 1111 Titrate samples to Time completed pH =4.50 S.U.
TL,..:-."irnltv and multiDlier determination:.
' f'Noirmality () of H2 SO I pHFactor or Multiplier:,
Deionized Titrant check Begin nd- (- ml Na 2 CO3 x 0.05)/E = (N x50000)/ 100 ml"samplet
..water re.eference standard ml ml ml =N IV 500
- 4.5 S.U- number number _____E) (aEceptablerang = 0. 0 Laboratory controlstandard:
Reference standard True value Sample Alkalinity (MV) % RS MV I TV z 100 number (TV) . volume Begin End Total Multiplier (mg CaCO31L) (acceptable range (mg.CaCOdL) (ml) ml ml_ ml I =90 to 110%)
100 1oo q.s6- bL.3' -
J Duplicatesam le precision: "_"_"_"_" "_. _',
Sample Alkalinity %RPD =
Sample Sample ID volume Begin End Total Multiplier. (mgCaCO3 /L) ((S - D) /(S+D)/21) x 100 number (ml) ml ml ml " " " (acceptable range =- 10%)
Duplicate (B) .- D K0 4
na+-eAro~ jciJvAqI-' 24%tVldAeAe Matrix soike recovery:
. . -- p Reference standard Spike value Sample Spike alkalinity (A)
(mg CaCO3IL)
I(mg number (SV) volume Begin End Total Multiplier 1A5S__b-*q CaCO3 1L) 10o(ml) 1r .-+ I+/-l ý.* m 0.3mlI t.tto, Sample alkalinity (B) Measured spike value (MV) % R =MV SV x 100 (gmI CaCO3.) I MV = A - B (acceptable range I' * " (mg CaCO31L) , = 75 to 125%)
ILO 100.0*
Sample measurements: -*
- Sample volume Begin End Total Alkalinity Sample number Sample [D (ml) ml - ml ml Multiplier . ni1 CaCO3/L)
,C.1X.1 i) 13,0 1.3 .4O.q JW**g, tr,..3, q.6 0.6 __" __,_.
"x 4.R", *'S / Io, 12.- 049 /G3- __'
______ L*tRiZM E--d*-* i-t.q 1?.0 ,.S ' o NLI-)o VWIN Ti 1,.0 I .sm I ,
\Ot'5 l1~ '7. 0~oZ __ _ _ _
U0__51 425
.A Page 64 of 102 Reviewed by: zI - Date reviewed: I A- I*
SOP C6 - Exhibit C6. 1, revision 06-29-09
I Page 18 Page-- -Of .5 SE-nvironn,"fi T. ngSoluin,Ionc..
Alkalinity- (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO 3/L
- Analyst F~C Time initiated Date analyzed,-[iii~ I Titrate samples to Time completed pH = 4.50 S.U.
-- Titrant norm i~a Dwjk~ate sam ie precision:
-- -- - "! Ie A lkalinity % RP D -
Sample Sample ID volume 1 End Total Multiplier (mag CaCO3 /L) {(S - D) /f(S+D)/21) x 100 number "_(mi) mi_ (acceptablerange_ 10%)
Duplicate (B) D Matrix spike recovery:
eren- value Sample Spike alkalinity (A)'
number (SV) vourE-"Begi-- Total Multiplier . (Ig CaCO3 IL)
(rag CaCOAL) (ml) ml mi mi-Sample alkalinity (B) .. Measured spike value (MV) % R =MV / SV x 100 (mg CaCO3IL) MV = A - B (acceptable range (mng CaCO 3/LI = 75 to 125%)
Sample measurements: .-
Sample volume Begin End Total. Alkalinity Sample number Sample 1ID (mi) ml ml ml Multiplier (mg CaCO 3/L) le.- 4 A93 118. - i _ _ .
oqLtfo.oI J k-j 2-\ 3,t6 3q.5-~ - ,zi ocilIO.. 64_v CAilO11~17-01_ ___ ____ L2 Reviewed by: I AI1 Date reviewed: I it.- 0is Page 65 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09
Page 2-1 P
Page 1 of_______
En~lmnme Iotn
-. s, Inc.
Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO3/L
- Analyst 6SL Time initiated 0 ro Date analyzed Titrate samples to Time completed I i2.
pH = 4.50 S.U.
Titrant normality and multiplierdetermination:
I Duplicatesam le precision: _ _" .
F. Sample Alkalinity %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CatOWL) ((S - D) /l(S+D)/21) x 100 number - (ml) ml ml ml (acceptable range = 10%)
Matrix spike recovery:-
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume. Begin End Total Multiplier (Ing CaCO3I-)
I(mnCaCO3 1L) (ml) ml nt,.
Sample alkalinity (B) Measured spike value (MV) % R = MV /SV x 100 (Ing CaCO 3/L) NMV = A - B (acceptable range (mg CaCOIL) = 75 to 125%)
Sample measurements:. _._ -
Sample volume. Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3 /L)
I1-2L.05 M114-S H-.k. o ____"_,
II- a9 ' .3 I ._ke_____2- _t4O 0I. 7-1. o 9 .0 2cI 110 O041116 O& Q- 1 I_" 4 .at.4 z. -L _ 0__r._ _
2i Reviewed by: Date reviewed: i 't"c.'O Page 66 of 102 SOP C6 - Exhibit C6.l, revision 06-29-09
Page R Ur* Page . ofZ5.p Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO 3/L
,.;,Analyst Time initiated Date analyzed Time completed ! ....
Titrate samples to pH = 4.50 S.U.
Titrant normality and mult*iier determination_- p ., Nomlt (N .f 2 5
--- - .Normality
- " Normality iN) o HSO4 4 4pHiip Factor orMultiplier .',
-Deionized Titrant check - - Dchu --TAUL = (5 ml Na2CO3 z 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml ml N x 0500
-4.5 S.U. number number (acceptable range = 0.0180 - 0.
- 1-1 iM j - . u 1
-. . I Laboratory controlstandard: ,
"Reference standard True value Sample Alkalinity (MV) %RSMV/TVXI10 U number - (TV)
(mg CaCO3 /QL volume (ml)
Begin ml End ml Total ml Multiplier (mg CaCO3-L) (acceptable range
-go
=' to 110%)
I I,ýS q,. 100 100 _. __.00 5 10_____.-_510o__
Duplicatesample precision: 1Ak tD I Sample number ISampleAlaiiy0RP Sample ID
-(ml) volume Begin M
End ml Total ml Multiplier (mg CaCOj/L) {(S - D) Il(S+D)/2)} x 100 (acceptable range I , Duplicate (B) 14+5 9 1 1Iog-k,-
S Matrix spike recover:.
Reference standard number Spike value (SV)
Sample volume Begin End Total Multiplier Spike alkalinity (A)
(mag CaCO31L)
(mgCaCO/QL) (mI) ml - ml .il " ,
I _ _ _J_1_
_ _ _ _ _ _ _ _ 5 10 4 S Z- f S 1 0f oi i Sample alkalinity (B) Measured spike value (MV) %R MV/ SV x 100 (mg CaCO3/L) MV = A - B . (acceptable range (Mr CaCO3jL) 75 to 125%)
I Sample measurements:_________
Sample volume Begin End Total Alkalinity Sample number Sample ID ml) ml. ml ml Multiplier (mg CaCO 3/L) o,*1 i'-P5 on 60D ý..*4.A Z2.q -5.1, I*.*£ Izo I
-3 I -~~.4 1 WAe 00ZV 6U.. 412 2 5 lc ~~i 11, .,o 5ID S.13)
I _ .__.'_ ,01 -5.3 11,0 5-1 . . 12,0 2 I not\1 I, .oS
.a-___
AI - 5k -1 a4 74 ~
4 Lo)
U1Ul~
_t,,
,Reviewed by: . Date reviewed: Iil-L I I Page 67 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09
S Page ,
9 Page & Ofz*.J_
9DETS
~ eootronn,.n aIT.otlvg Sou 015 *~C Alkalinity (SM 2320 B)
Matrix: Water, RL - LO mgCaCO 3/L Analyst 5, Time initiated
.Date analyzed. I i IIII] Titrate samples to Time completed r, Vý pH = 4.50 S.U.
Laboratory control standard:
Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin- End Total Multiplier (mgCaCO3/L) (acceptable range (mg CaCO 3/L) (ml) ml ml ml =90 to 110%)
z- , 100 1o0 d4. oz.
02 ...
Duplicatesamn le precision:
Sample Alkalinity %RPD Sample Sample ID volume Begin End. Total Multiplier (Ing CaCO3/L) ((S - D)/[(S+D)/21) x 100 number (m_l ml ml ml II (acceptable range =* 10%)
S q 3 5.
- 394 fl4 2 Duplicate (B)- . Djyj10, Matrix spike recovery:
- Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Ing CaCO3/L) 4 (mg CaCO3/L) (ml ) ml ml ml
-too J qv .
'A), a(i) o /I,O Sample alkalinity (B) Measured spike value (MV) % R = MV/ SV x 100
.(mgCaCO3/L) MV=A-B (acceptable range
. (mIg CaCO3/L) = 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number SampleID U) (l. ml ml ml, Multiplier (mg CaCOs/L) o4 11 rlIf 1 :60 6,0 34I 3,1 12U104 0i 1 .t04 7 L. ( J'o 011,11 -0S-3. - &A 2.9.1 orlll.. S toio k' 1 17,0 3.2 CA.i_ ._ - - J.LQ1,Q dA,.. 2-0, U_'
-3 ___0 A-1 -61_
Q~iLLVASCL IL 6
) 1rtw I _ -o .3~.1 - __ (I(
0,1iJ17-. us_
olItlO, oS 3&.
3 ___,__
1ý
- o, 3,o(.,
____ _____,_1
.Reviewed by: Date reviewed: I I\v2Z-1 0*
Page 68 of 102 SOP C6 - Exhibit C6.1, revision 06-29-09
6 Page -4 0
Page _*of 0JETS
~ En~ronmrnta Twing Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst Time initiated Date analyzed .t O Titrate samples to Time completed pH 4.50 S.U.
Titrant normality and multiplierdeterminatloi
-,'Normality'. S. " . .Normality (N) of HSO - pH-Factor or Multiplier n.... '- check Begin End Total = (5 ml Na 2CO 3 I 0.05)/E (Nx 50000)/ 100 mnl samp'le water ml ml --0.25/E = Nx 500 S4.5 S.U. number number -: - (acceptable ranee = 0.0180 - 0.0220)
T. ----
- Laboratory controlstandard:
Reference standard
- True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCOgL) (ml) ml ml ml =90 to I10%)
- -f3t4. I100 100 . , . /0I3 0,,
H Duplicate sam le precision: . ,.
Sample . Alkalinity %RPD Sample ' Sample ID volume Begin End Total Multiplier (mg CaCO3/L) [(S - D) /[(S+D)/21) x 100 number _ml) ml ml . ml (acceptable range 10%)
IS
____Duplicate(B 0 Matrixspike recovery: __"
Reference standard Spike value Sample Spike alkalinity (A) number (SV) - volume Begin End Total Multiplier (Ing CaCO3/L)
(mg CaCO3/L) (Ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R =MV I SV x 100 (Ing CaCO3/L) MV = A - :.(acceptable range (Ing CaCO3/L) =75 to 125%)
Sample measurements:*
Sample volume Begin End Total - Alkalinity Sample number -Sample 0ID (ml) ml ml ml Multiplier (mg CaCO 3/L) o 011 lgA . o -Z1- 6,Qn 21 L1'( 7J q_ 14) C).-+
4 2,o q7, 0__ 1 o, 01 ', .IL9.
ie.1, i 7- io &-, l-IL41 to eiw I JL 30. 0 0117._. o %*03 16_,0 A t0 '
Reviewed by: Date reviewed:
Page 69 of 102 SOP C6 - Exhibit C6.11 revision 06-29-09
Page *5 of _ __
P age Fnvironme,,
alTemngSoludons, Inc.
. Alkalinity (SM 2320 B)
Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst. -+< i4 j,,] Time initiated
'Date analyzed ". I.. Titrate samples to Time completed pH = 4.50 S.U.
,Tltr4 Mff~ .v " " "erdeterrainattot U - * - * - a- . - . - . a
- Normality. .,;;,Normality.(N) of HiS0 4 -m;,pH.Factor
. orMultiplier:
Deionized .,Titrant check. Begin End- Total - azCO.sX0.05)/E = (NxSOOOO)ll00mlsample water reference standard ml ml ml =NxV 500 4.5 S.U. number number (EM (acceptable range = 0.0180 - 0.0220) 11
,L -L . ._ _. - _ __.. ... ...
Laborator controlstandard:
Reference standard True value - Sample Alkalinity (MV) % RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (g CaCO3/L) (11) nil ml ml -90 to 110%)
S -
2 . 10 0 too 31.8 11 j 53 10.1 100, tOO'/.
Duplicate sapple precision: ._.
Sample I.* Alkalinity %RPD =
Sample , Sample ID volume Begin End Total Multiplier '(mg CaCO3/L) {(S - D)/[(S+D)/21} x 100 number (ml) ml ml ml 1! (acceptable range = 1 10%)
_____ W IZI W) 2. 100 hqA,. 31.4 0-2 C Duplicate (B) tej j D5 D
Matrix spike recovery: _ _ __._ _
Reference standard Spikevalue Sample Spike alkalinity (A) -
number (SV) volume Begin End Total Multiplier (mg CaCO3IL) r (mg CaCO 3/L) (ml) ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV /SV x 100 (mg CaCO 3/L) MV = A - B (acceptable range (In CaCO 3/L) 7 5=to 125%)
zO
- IOo 1-Sample measurements: ______
Samlple Volume Begin End Total. Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) 4 4A S 2- 3qo,oq.6 3646 1.0 0o .S _. (,;
Reviewed by: Date reviewed: I c Page 70 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09
Page _ _
Page (0 of
)EnvlronmentalTestlng Solutions, Inc.
Alkalinity (SM 2320 B)
Vdatrix: Water, RL = 1.0 mg CaCO3 /L Analjyst% Time initiated Date analyz~ed l Titrate samples to Time completedI.1III_ IIL pH = 4.50 S.U.
-,_,Tltrant normality nd multiplierdetermination:
Deionized- TtRitrant check
- Begin - E-U I S-Normality'(N) of H 2 SO 4 : -' : pHFactor or M6ltiplier- 1 nd Total =,(5 ml NaiCO 3 x 0.05)[E = (Nx 50000)/100 ml *ample water e"e standard ml ml ml = 0.25/E S =Nx 500
. I1R (accentable ranee = 0.0180 - 0.0220*
-4.5 S.U. '0e, number' numb_______________ I (acceptablerange_=_0.0180_-_0.0220)
Laboratorycontrol standard:
Reference standard True value .lwe Alkalinity (MV) % RS = MV / TV x 100 number (TV) volu ' Begin End Total Multiplier (mg CaCO3IL) (acceptable range (mg CaCO3/L) (ml) ml ml ml =- 90 to 110%)
. 100 100 Duplicatesam le precision: __"""
Sample Alkalinity. %RPD=
Sample Sample ID volume Begin End Multiplier (mg CaCO 3/L) ((S -eD)l [(S+D)n21) x 100 numer ____________________ _Val__ (acceptable range 10%)
Duplicate (B) D _
Matrix spike recovery:
Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOsIL)
(Mg CaCO 3 /L) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R = MV/ S 100 (mgMV = A- B (acceptable rangeb, (mg CaCO3/L) =75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID -(ml) ml ml ml Multiplier (mg CaCO3IL)
_taolA t)Lf~$ L(2 .M. 44 -I JL L (
11 M6__1 1 _&& _
3_" 36_16 5;-Y Reviewed by:, Date reviewed: I i-o9 Page 71. of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09
0 Page ["__'
S
~
I~ETS 0
Envmn~nta Tat ig Sou on. nc Page of C
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO 3/L Analyst _ v)I l.% Time initiated Date analyzed . Time completed t ed Titrantnormality and multiplier determination:
Titrant Normality check Begin End Total' Normality (N) of EDTA- -. pH Factor or Multiplier.-
,reference standard " ml ...... ml ml " 0.2/E - " = (N x 50000)150 ml sample' number. number (E) (acceptsbit range = 0.0180 - 0.0220) =Nx 1000
'lt ')!,Z- iA&sstl'k 0,o 10-0 i10,0 7~z z Laboratory controlstandard:
Reference standard True value Sample Hardness (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total -Multiplier (ing CaCO 3/L) (acceptable range (rag CaCO3L) (ml) ml ml ml .= 90 to 110%) j
[,SS7'02. 40 50 %O,0 t12-,.3 7..0 'A0*. 1O00.0"1, Duplicatesam ple precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (rag CaCO 3/L) {(S - D) /[(S+D'2jl X 100 number " (ml) ml ml ml rx o NNS01
'il HS) 6 12,a) LA'*. 20-0 S Duplicate (B) 1. 5 D C10 Afatrix spike recovery: "__ _
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)
(mg CaCO3/L) (ml) ml ml ml 4* o I- leo Sample hardness (B) Measured spike value (MV) % R = MV/ SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (Ing CaCO 3/L) 75 to 125%)
IOo oo:o.
Sample measurements: +-
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)
TV= ND Blank (should be =0 m CaCO 3/L) 6_o v__) 0o 7,_0"0 I1 3 O'~*~(~s W6z ___S 'a '-S
-IS-___
I v. .A4 A" 52.O 3:_-_ _._._
0,1__ .__
_0rr1. iS 4z~z 0,3. 6.0
.',Oat -t..k Ee ?-la 00 N)~ 390i:.
611IMIbll-M "I ill ,,0Z A__ 3A %La7-183 Pigs 172V1JiMF 2 trant is used, sample must be diluted. Reviewed by: CAis bate reviewed is -09 SOP C7 - Exhibit C7. 1, revision 06-29-09
Page 16 0t 0*,
Page 2- of2 Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO 3/L Analyst [ uIx.I Time initiatedI Date analyzed-[.1L .0Ih1 Time completed 7 1 FI. , ag. alit and multiplierdetermination:.
Ttrant .Normality eh-e' -Total - .Nrmlit (N)ofEDTA pH Factor 6r Muitipiiei reference standard ml ml m1 = 0.2/E = (N x 50000)1 50 msi-ample i number number (E) (accepta - 0.0220) = N x 1000
-Laboratorcontrol standard:
Reference standard True value Sample, Hardness (MV) % RS MV /TV x 100 number (TV) volume Begin End Total Multiplier (01g CaCO 3/L) (acceptable range (mg CaCO 3/L) (ml) ml ml ml =90 to 110%)
I As S"b..0 50 qj 2,0. 10. 40 10),01'-
Duplicate -samle precision:_____________________
Sample Hardness " %RPD=
Sample Sample ID. volume Begin End Total Multiplier (mg CaCO 3IL) ((S - D)4(S+D)/21) X 00
! number
' *_.o (ml) ml ml 12Z.
ml
'5 s to Duplicate (B) 1 12.5, 1.0,C D 10
. Matrix spike recovery: _
Reference standard Spike value Sample Spike hardness (A)
I! number (SV) volume Begin End Total Multiplier . (mg CaCO 3/L)
(mg CaCO3 /L) (ml) ml ml ml Sample hardness (B)- Measured spike value (MV) % R = MV / SV x 100 .
(mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75. to 125%)
tO 4c) too-ol.
Sample measurements: .
i Sample number TV
- Blank Sample [D (should be = 0 mg CaCO 31L)
Sample volume (ml)
,0 Begin 1.
ml End ml 1,.2-Total 00 ml Multiplier 0 ..
o Hardness (mg CaCO3/L)
I * " lU-Wk'i'°\W, Sh,,,n 16-3 in.L 1.I -,.1, I
I I. pý*,:'*3"kWY6trant is used, sample must be diluted. Reviewed by: Date reviewed i SOP C7 - Exhibit C7. 1, revision 06-29-09 IJ
ETS 0 Page I0 S.
Page _ _of *"
9
~nnitwtntwtei Testing p
Total Hardness (SM 2340 C)
RL = 1.0 mg CaCO3/L Analyst 6S7L Time initiated 17~l7 i :i77I Date~analyzed .O Time completed '
I
.Ttrantnormally and.multiplier determination:
Titrant- Normality-check.. Begin- End Total !-Normality(N) of EDTA pH Factoror.Mu!tiplie4*,_:`.I reference standard ml ml ml 0.2/E = (N X 50000)/50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220) = N x 1000
&2c [JS 1.7O ' I ,LI .-
Laboratory control standard:
Reference standard True value Sample " Hardness (MV) %RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier r(mg CaCO3IL) (acceptable range (mg CaCO/UL (.mn I ml ml ml =90 to 110%)
(rJ5S 7-02. 40 50, 1o..
- 0. , q q '0.9 Duplicatesam ple precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3IL)" {(S- D)/I(S+D)/21) x 100 number .. _ (ml) ml ml ml
- Duplicate (B) .I . w 4 . ID Matrix spike recovery:. . ..
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mag CaCO3/L),
(myCaCO/L) (ml) ml ml ml Sample hardness (B) Measured spike value (MV) % R = MV /SV x 100 (mng CaCO3/L) MV = A - B (acceptable range (Mg CaCO 3/L) = 75 to 125%)
Sample measurements:
- "Sample volume Begin End. Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier' (m01CaCO3/L)
TV =ND " Blank . .
TV N (should be 0 mg CaC0 3.L) So____13,_____ t14 tl-yl.s WWH t 13,,,.o Z4'-. 1 4.1 1 '84 MI-0~9 GSL~j __ ~ A.
2- 30.0 - *.-, -A
___ _ _ F\W .... V.) ,>-.y, 4-.2. - 6 SIII I PaWe 1 t§,Awirant is used, sample must be diluted. Reviewed by: -Ik Date reviewed 1 1iI2Z-SOP C7 - Exhibit C7.l, revision 06-29-09
Page "o Page_ of
'V'~U Tr.~J -~ IC'1IE ~i~Afl r~\
I otall Hardness talvi 4340 Q) 77:7 RLO*.0 mg CaCO3/L . .- *.,
S -i "Time initiated' Date a.a.. z _,*
i, Time complet:ed -
. r...t*..vrmal***andmultiplierdetermination:
v
- " .*irmi**. ite * .End Total. Normality (N) of EDTA pHFactor.orMultiplier reference .0.21E standard ( &50000)/ 54it sample number. nlUllm~e.* laccin a-0.20 .N 1000*v Laboralor, controlstandard: ......
Reference standard True value Sample Hardness (MV) % RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO/) (ml) ml m ml =90 to 110%)
Duplicate sample precision: "_'"
Sample Hardness %RPD Sample Sample ID volume Begin End Total ml Multiplier. (mag CaCOIL) AS(S - D) /[(S+D)/21} x 100 (ml) ml ml number 0"i I 20 - o Vefr-p1 , 10 n4. I -l At 4 S~ G bC-66 Duplicate (B) 4 , *.D -. tg0 3 I Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Mg CaCO3IL) ml ml
- s*. (mg CaCO 3/L)
.;° , " (ml)
- o. . ml
.L,*- 0. 6 (.5),44 16-o Sample hardness (B)' Measured spike value (MV) % R = MV / SV x 100 (mg CaCO 3/L) MV-= A - B (acceptable'range (mg CaCO3/L) = 75 to 125%)
Sample measurements:_________
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml .'ml Multiplier (mg,CaCO 3/L)
TV =.ý . Blank Z je 41 -- _1__i_ 140 _ 3 0Aý 10.O 3- )
4 5A
- 34 I51
=~-I.+
(31%mg-L iAL RF 002-o t'?A
- :. a nn -9 0cj, it It 0fiffj I" U I*~ j).% - I . 1qq oai% it. it) Rig I 7_1 B __. I LO
- 0O1.1%& 05 4 Jno 5 bef~ W/ ant is used, sample must be diluted. Reviewed by! i t .I Date reviewed iE l:I SOP C7 - Exhibit C7.1, revision 06-29-09
Page ZI 1
Page 3 qf
-4
,FT5 --
Total Hardness (SM 2340 C)
RL = .0 nmg CaCO 3/L Time initiated i
Analy.st
.. an-alyzed- .Date -ii oII]- Timecompleted Laboratory controlstandard:
Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOA/L) (acceptable range ml ml ml =90 to 110%)
a=s*_40 (mg CaCO3/L) (ml) 50 1.5 1-45 1-.:i.5 r+- Ca. 1 Duplicatesam pie precision: '
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L). ((S - D) /[(S+D)/21} z 100 number (ml) ml ml ml * '
Duplicate (B) !.- ., T D
-- ,,* I - ) -c,4 05 Matrix spike recovery: "3,,
Reference standard Spike v'alue Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier '(mag cacoy/l.)
(mg*CaCO 3 f/L) (ml) ml ml ml Sample hardness (B) Measured'spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV= A - B (acceptable range (mig CaCO3IL) 75.to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO3 fL)
_______ (should be =00 mg CaCO3 /L)
'Blank f.t*j0(*\ _ In
_.45-r _,,o_ _ 0 12) __ _
o, 0______O-_0
,-4, -,,,'. i UU 3
z
____o_
.a1z. q
- ,v,- l U'3 01I1111.65. 1(tA*So 161-i~y U, 1 c
_____o__S* CA4 1 0:1 *, .1 3-3
,.a*g -4 ,-f3*.' JI..
jaI - ..:
_ to oit2.~3 -#ý2_ _____ CP 5__ _
PA":Jr6l-*IYJtrant-is used, sample must be diluted'." Reviewed by:, J " .-- Date reviewed ,
. (* I
- .SOP C7 - Exhibit C7.l, revision 06-29-09
S Page QA 0
3 ET~
0S
~ Enyronmena Testng Ions nc Page '4 of 4 Total Hardness (SM 2340 C)
' RL = 1.0 mg CaCO 3 /L Analyzst [7- ,- 11,- Time initiated Datiiahalyze~d i1 I] Time
-. completed
.....Titrant normality and muitinlier determination:
kiii.ý L*gin L- n Total .. .. Normality'(N) of EDTA , .. pHýFactoror Ma1tiplieý!ý:ý" */*"
.-reference standard ml ml ml (N x 50000)150 ml sample ...
number number (E) ane008Socpal 0.0*lF-"-'---N 1000 Laboratorn controlstandard: "
Reference standard True value Sample Hardness (MV) % RS =MV /TV x 100 number (TV) volume Begin End Total Multiplier (Ing CaCO 3/L) (acceptable range-(rag CaCO3/L) (ml) ml ml ml 90 to 1t0%)
Duplicate sam Je precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (fag CaCO3/L) ((S - )[(S1D)/21} I 100 number _ml) ml ml ml Duplicate (B) D Matrix spike recovery:
Reference standard Spike value Sample Spike hardness (A) number V (SV)
"(mgCaCO3/L) volume (ml)
Begin m[
End ml Total ml Multiplier (Ing CaCO3 /L)
'Sample hardness (B) Measured spike value (MV). % R = MV ISV x 100 S (Mg CaCOi/L) MV = A - B (acceptable range S "(mg CaCO 31/L) =75 to 125%)
Sample measurements:-
-Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)
TV = (slak
/
jf* d4' I- -" ll, [ 1.
t*)R,£i,-*"htm , I3.'l I*. j.&o*
Ii Atgelf7>2 bJY+Y 4~-~LLLiI ant is used, sample must be diluted. .Reviewed by: lZ IIII l.* Date reviewed . ! " j SOP C7 -Exhibit C7. l, revision 06-29-09
U7 Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix D Reference Toxicant Test and Control Chart
-Page78 of 102
- I Pimephalespromelas Chronic Reference Toxicant Control Chart.
D Organism Source: Aquatox, Inc,.
Environmental Testing Solutions, Ic.
USEPA ControlLimits (+ 2 StandardDeviations) ]
1.0 0.6 I .. .. . . .. . . .. . . . . . .. . . . . . .. .. . . . . . .. . . .. . . . ..... .....
0.4 I-19, L J*
.USEPA Warningand ControlLimits (75t2and 9(/ .Percentile CVs)--
Q 1.0 0.8 Q 0.6 0.4 N
I I I I t I I I t I t t i t I t I I t t 1.2 I I I I I I I I I 1 JI I1 I I I I Laboratory Warning and ControlLimits(10J and 25 Percentile CVs) 1.0 0.8 0.6 . . .. . .. ... .. .. .. . .. .. .. . .. . .. .. .. . .. ..
0.4 L l .
Test date 7-day IC25 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- *- Central Tendency (mean IC 2 )
. Warning Limits (mean IC 25 +/- S A.0 or SA.75)
.......... P.PgCntr 97 Limits (mean IC 25 +/- SA.25, SA.907. or 2 Standard Deviations)
ETS Environmental Testing Solutions, Inc.
Pimephalespromelas Chronic'Reference Toxicant Control Chart State and USEPA Lalboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT S Control Limits . S, Warning Limits S&25 Control Limits SA75 Warning Limits SAM Control Umits Cv (g/L KCI) (g/L KCI) CT -2S CT + 2S CT-S,.10 CT+St, 1 CT-S-25 CT+SC.2I CT - S,.7 . CT + SAMs CT-Sk CT'+ S,"
I 02-25-09 0.76 2 02-27-09 0.72 0.74 0.03 0:68 0.80 0.09 0.65 0.83 0.15 0.58 0.89 0.28 0.46 1.02 0.33 041 1.07 0.04 3 03-03-09 0.75 .0.74 0.02 0.70 0.79 0.09 0.65 0.83 0.16. 0.59 0.90 0.28 0.46 .1.02 0.33 0.41 1.08 0.03 4 03-10-09 .0.79 0.75 0.03 0.69 0.81 0.09 0.66 0.85 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.04 5 03-10-09 0.77 0.76 0.03 0.70 0.81 ' 0.09 0.67 0.85 0.16 0.60 0.92 0.29 0.47 1.05 0.34 0.42 1.10 0.04 6 04-14-09 0370 0.75 0.03 .0.68 0.82 0.09 0.66 0.84 0.16 0.59 0.91 0.28 0.46 1.03 0.34 0.41 1.09 005 7 04-21-09 0.80 0.76 0.04 0.68 0.83 0.09 0.66 0.85 0.16 0.60 0.91 0.29 0.47. 1.04 0.34 0.42 1.10 0.05 8 05-05-09 0.69 0.75 0.04 0.67 0.83 0.09 0.66 0.84. 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0,41 1.08 0.05 9 05-12-09 0.75 .0.75 0.04 0.67 0.82 0.09 0.66 0.84 0.16 0.59 0.90 0.28 0.46 1.03 0.34 0.41 1.08 0.05 10 06-09-09 0.76 0.75 0.04 0.68 0.82 0.09 0.66 0.84 0.16 0.59 0.91 0.28 0.46 1.03 0.34 0.41 1.09 0.05 II 06-18-09 0.78 0.75 .0.04 0.68 0.82 0.09 0.66 0.84 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 12 07-07-09 0.72 0.75 0.04 0.68 0.82 0.09 0.66 0.84 0.16 0.59 0.91 0.28 0.46 1.03, 0.34 0.41 1.08 0.05 13 08-04-09 0.78 0.75 0.04 0.68 0.82 0.09 0.66 0.84ý 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 14 08-11-09 0.81 0.75 0.04 ,0.68 0.83 0.09 0.66 0.85 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.42 1.09 0.05 15 09-15-09 0.72 0.75 0.04 0.68 0.83 0.09 0.66 0.84 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 16 10-06-09 0.78 0.75 0.04 0.68: 0.83 0.09 0.66 0.84 0.16 0.60 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 17 10-14-09 0.77 0.76 0.04 0.68 0.83 0.09 0.66 0.85 0.16, 0.60 0.91 0.29 0.47 1.04, 0.34 0.42 1.10 0.05 18 10-28-09 0.73 0.75 0.03 0.68 0.82 0.09 0.66 0.84 0.16 0.60 0.91 0.29 "0.47, 1.04ý 0-34 0.41 1.09 0.05 19 11-03-09 0.67 0.75 0.04 0.67 0.83 0.09 0.66 0.84 0.16 0.59 0.91 0.28 0.46 1.03 0.34 0.41 1.09 0.05 20 11-17-09 0.79 0.75 004 0.67 0.83 0.09 0.66 0.84 0.16 0.59 0.91 0.29 0.47 1.04 0.34 0.41 1.09 0.05 Note: 7-d IC, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean ICs).
S = Standard deviation of the IC25 values.
Laboratory Control and Warning Limits
-Laboratory control and warning limits were established using the standard deviation of the lCas values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
SA.IG = Standard deviation corresponding to the 10'h percentile CV. (SA:O-I0.12)
S,. - Standard deviation corresponding to the 25'h perccntile CV. (SA,2S= 0.2 1)
USEPA Control and Warning Limits 5
= Standard deviation corresponding to the percentile CV. ( 75 = 0.38) "a 75 Sg.j= Standard deviation corresponding to the 90 percetile CV. (Sg = 0.45)
CV = Coefficient of variation of the IC2s values. ,-"* .. :, i .:"-':
USEPA.2c0D.tlNnd .aWAewuo59 fbrMehdWVauiadY o WhOleEmoanf Tonmy Appbli,.Ms Uodff the Naiiroal PollutamDischage Eblonjaia Proprarn. EPA-t33-R-00-003. US E-m mSidi armiat oftdima Agaeny,Cnnria. OR-Organu.sobsamdfrom Aquat". Ine. atoxl 1-17-09
0 Precision of Endpoint Measurements D ETS 3F ý *Chronic Pimephalespromelas Reference Toxicant Data Environmental Testing Solutions, Inc.
Control Control Mean Test number Test date
.. Survival Growth. CT CV CT . -MSD PMSD CT for Conirol Growth for Control
(%) (mg/larvae) (mg/larvae) (%) Growth CV (%) (%) for PMSD (%)
02-25-09 1oo 0.659 8.2 0.07 11.1 13.1 2 02-27-09 100 0.578 0.618 6.8 7.5 0.08 12.1 3 03-03-09 100 0.664 0.634 8.1 7.7 0.08 12.3 12.2 4 03-10-09 100 0.661 0.640 6.8 7.5 0.09 12.9 12.4 10-09 5 100 0.696 0.652 8.8 7.7 0.09 12.6 12.4 6 04-14-09 100 0.671 0.655 10.3 8.2 0.08 11.4 12.3 7 04-21-09 100 0.642 0.653 8.3 8.2 0.07 11.2 12.1 8 05-05-09 100 0:805 0.672 7.9" 8.2 0.07 8.4 11.6 9 05-12-09 100 0.717 0.677 7.0 8.0 0.06 8.9 .11.3 10 06-09-09 100 0.703 0.679 2.5 7.5 0.08 10.7 11.3
- 11. 06-18-09 100 0.790 0.689 6.7 7.4 0.09 11.7 11.3 12 07-07-09 100 0.763 0.696 5.7 7.3 0.1 1 14.2 11.5 13 08-04-09 100 0.692 0.695 6.2 7.2 0.07 10.7 11.5 14 08-11-09 1oo 0.583 0.687 8.0 7.2 0.05 9.3 11.3 15 09-15-09 100 0.723 0.690 9.6 7.4 0.08 10.4 11.3 16 10-06-09 100 0.894 0.702 12.9 7.7 0.12 13.2 11.4 17 10-14-09 97.5 0.758 0.706 7.6 7.7 0.14 18.3 11.8 18 .10-28-09 100 0.855 06714 6.5 .7.7 0.10- 12.0 11.8 19 11-03-09 100 0.757 0.716 7.1 7.6 0.07 8.8 11,6 20 '11-17-09 97.5 0.825 .0.722 8.3 7.7 0.10 11.8 11.7 Note: CV Coefficient of variation for control growth.
Lower CV bound determined by USEPA (10th percentile) 3.5%.
Upper CV bound determined by USEPA (90th percentile) - 20%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (10' percentile) = 12%.
Upper PMSD bound determined by USEPA (90"' percentile) = 30%.
CT = Central Tendancy (mean Control Growth, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole EffluentToxicity Test Methods, Volumes I and 2 Appendix. EPA-82 I-B-01-004 and EPA-82 I-B-01-005. US Environmental Protection Agency, Cincinnati, OH.
Organisms obtainedfromAquatox, Inc. atoxl 1-17-09 Page 81 of 102
0 "ET
- .:,'l Environmental-Testing Solutions, Inc.
I.Lfl Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.
1.00 0.75
.4- - * ' I~ - I I I I I I I I I I I I I I I I I 0.50 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) 30 Kentucky Acceptance Limit (< 30.0%)
- .20 o 10 o 0
- i I I I I I I I I I I I I I I I I I I I I* I I. I I I I I I I I I I I I .
30 USEPA Upper PMSD Bound ( 9 0d1 percentile < 30.0%)
a 20 rj~ ..................
10
-1 I ~ i*~l ~ I ~ I I I -
I.1 II I~*
I -
Ii I1 II Test date
- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- -* Central Tendency (mean Control Growth, CV, or PMSD)
...Pagv* t kllInmits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
I i S S
Page I of 5 Ii EnTS,nT. g I Potassium Chloride Chronic Reference Toxicant Test (EPA.-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas pKCICR Test Number: I__
Dilutionpreparationinformation: Comments:
KC] Stock INSS number: It.) 1" "41 ._'_*_-_
Stock preparation: 50 g KCL/L:
Dissolve 50 gKCI in 1-L Milli-Q water. ..
Dilution prep (rg/L) 450' 600 750 900 1050 Stck volume (mL) 9 .12 15 18 21 Diluenitvolume(mLF 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information:
Organism age: -.- NS Irkoju.Z 0%-b Randomizing template: ?i A-..
Date and times organisms -\\-*-a-* *Oc Incubator number and were born between: shelf location: .
Organism source:. -- , ,, Artemia CHM number: - t'* LI4LI Drying informationfor weight
" __determination:
0 Transfer vessel pH = S.U. Temperature = C Date / Time in oven: l r'rA-o* %%%(b information: 11". (0 Initial oVen temperature* too ..
Average transfer volume: Date / Time out of oven: IL-181411 IlqO
- 0. Final oven temperature: - t.* C Total dyinzg time:
Dailyfeeding and renewalinformation:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW i
i .
0 i'~ioj I-__q -0e ~
Time a
Analyst AL Time
\t~
Analyst or termination Time.
LtO 0~t' IVA_____
Analyst batch used.
o2 I 1- .10-601 x00-t Od 00 a o Ok&
1"40 . - Iq.b
%TzVL.0 7\-K4, wtz'.lq - *%L*
V.oact'-6 b
I&
5 l-a-oi v---oi O.&oG I4 lqoa%AO.v AL 1210* '~o 6 L%-L%-01t h ,i , t"%bo Id %-LZz,
-2r, I.t 7 ý1%-2+01 "ti Control information: Acceptance criteria Summary of test endpoints:
% Mortality:
Average weight per. initial larvae:
Average weight per surviving larvae:
I.8 O.gqS 1 7, " :, 20%
0.25 mg/larvae' 7-da, LCso NOEC LOEC0 j 91 Ri(44 (000 ChV (.10.9 IC25 1 -797-3 Page 83 of 102 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09
0 Page 2 of 5 0
S 9
J En*on.,,.,,la T*. nqSols,0.0.
Species:' PimephalesDroMelas PpKCICR Test Number: 195.
Survival and Growth Data "I A Day
.0_.____ " A B Control C D E 450 me KCV/L F G H I 600 mg KCVL J K L
/0 '0 /0 to /0 /0 /0 /t /0 /( /0 1t (0 /0 /0 t0 /0 t6 (0 /0 /0 /0 /0 /0
/0t) to 10 10 /0 10 "10 10 .10 10 I0 .10 3 I o /! /0. 10 /03 /O /03 /0 /0 /!0 /0 4(I0 tO /0 O I1/0 U / (0 &O I0 4 (1 10 10 ,' 1k to 10:) 10 10) t O1('to
- 6. 5i 10 .O10 ~. 10, 10 1. 0 /1 10 10 10
/3, 1I ~ I______C) 10 10 V4 'c0 10 'V0
-o - at 'VA- I-iIo t 1 - S A Pan weight (mg)
Tray color code:i"Y410.)
Analyst: (. . l> I . Sto IS.7, l'- j ' P 7. 1i,4 I . , -- I .-I /q.s o(4.),* i'.I.1 Date:, - "-Z2,- "-
B = Pan + Larvae weight Analyst: %e-Z,*
(mg)
- 4 .1 Z.* 7,.7 Z-.02-Z. 7.
Z.*
- T-4 2.0' 7, 0 Z 6 -*\Z \Zý .
Date: IL_.t01. o- Z2 C = Larvae weight (mg)
= B - A. 6. -% ' e 5 ' A Weight per initial number of larvae (mg) A. N 5
- C / Initial number of larvae b AI, 0, ~* 01 0*
I -
Average Percent
-I R3 I - S. X7.-~
weight per reduction initial from control 0 SVI.5 number of (%)
larvae (_E)
Comment codes; c = clear, d = dead, fg= fungus, k = killed, m = missing, sk sick, sm = unusually small, Ig ý unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments: "
C. Page 84 of 102 SOP AT21 - Exhibit AT2 1.1,revision 04-01-09
Page 3 of 5 F
3 DTS Species: Pimephalespromelas PpKCICR Test Number: 1 ..
0 1(3J() , 1o Survival and Growth Data _ _ _..
SI___
Day 00 1
M 750 me KCI/L
- q 10 N ._
M 0
oak Ot I 0 SA 900 me KCVL R
/l0 S
to o to'1o T
Ut U
to/,
wk 1050 m KCVL V
q W
4 10
ýX 1o 2 o- 1 0 5 -1 s 70 01 C) S1
- 5. S (
4 5--
_~~ 1A~
.7 g 4- i R A = Pan weight (mg)
T ray color code:: )A4O& K lq-.'4b 14FI3 Analyst: -4A.,t l'f,,Dy 13, 4...l5..?Zl",L*
) 7-, I3 Date: IO"L 'O., -
B = Pan t Larvae weight Analyst:
Date:
VA. Z O.2 c z2-M3 tO. *Al- eq.5q iI.ýai'. i*.73 I(L.q2 ')s Ii,.'(
i "Z.01-01)'
C = Larvae weight (mg)
,= B-A - t . &.q SR . 4t 11, q. S S L.OS. &b 'ZO 3 &A IS S 1,ta 1.-.63 1.', 1 Weight per initial number of larvae (mg)
SC / Initial number of larvae ) ' * ' *"
- X%'O
- "*N" .*
Average Percent.
weight per reduction -61 0.
initial from control O:'S 'a.'.-
number of (%)
larvae (mg) .f""
Comment codes: c= clear, d dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Page 85 of 102 .. SOP AT21 - Exhibit AT2 1.1,revision 04-01-09
Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Teat number. PpKOCI #193 Environmental Testing Solutions, Inc. Teat dates: November 17-24, 2009 Reveiwed by:
(maL Coaceantonlmo Replkale d1aiaumbetra Fotlnumbered A Pm. eighl B=Pu+L.a. Larvamewght (mg) WewSnghs inSg Mew elgah/ Coefflde.5 varlal. Weldghsllciddasber Mean narviv,.1 leC *m.hld al ~
"ele kt d Pertcat red*t*d.ewu KCI) la e lar-e (ow , wefght (.W A -AB unite-dlarvae(mue S .. riving ollmb or,r oi,. etw
- ofhtume-(n) 1W%) eaabe-6larvae vuariatio Y) control (1/)
.: larme (mg) ,m*b*,, (t) (.ie A 10 to 13.90 . 22.97 9.07 0.907 0.907 B to 10 15.09 23.14 8.05 0.805 0.805 Cotrol C I0 10 13.58 22.01 8.43 0843 5.2 0.845 043 9 D 10 9 13.21- 20.65 7.44 0.827 0.744 E 10 9 14.99 22.02 7.03 0.781 0.703 430 F 10 10 13.74 *21.33 . 7.59 0.759 07803 5.2 5997.5 0.783 A 5.1 G 10 10 12.68 21.22 8.54 0.854 0.854 H 10 10 12.51 20.67 .8.16 0.816 0.816 .
1 10 9 14.11 21.90 7.79 0.865 0.779 60J 10 10 14.50 22.98 8.48 0.848 0.948 93 6K 10 10 14.06 21.21 7.15 0.71519. 0.826 0.715 98.0 ,.783 7.0 I L 10 9 14.34 22.23 7.89 0.877 0.789 M 10 8 13.48 20.32 6.84 0.855 0.684 N 10 7 14.64 20.95 6.31 0.901 0.899 4.6 0.8391 77.5 "98 15.9 15-3 750 0 10 9 13.84 22.43 8.59 . 0.954
_P I0 7 14.21 20.40 6.19 0.884 0.619 Q 0 4 14.79 19.12 . 4.33 '1.083 0.433 R 10 5 15.29 19.54 4:25 0850 0.892 14.4 0.425 42.5 0.378 15.7 54.2 S 10 4 13.62 16.82 3.20 0.800 0.320 1
T 10 4 14.31 17.65 3.34 0.835 0.334 _
U 10 2 15.18 16.73 1.55 0.775 0.155 V 10 I 15.72 16.90 1.18 1.180 0.894 218 0.118 17.5 . 0.149 15.0 81.9 100 W 10 2 14.42 15.95 1.53 0.765 0.153 X 10 2 14.73 16.44 1.71 0.855 1 0.171 Dunoett's MSD value: 0.097s MSD = Minimum Significant Difference PMSD- 11.8 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimut percent difference between the control and treatment that can be declared statistically significant in a whole effiueit toxicity test.
Lower PMSD bound determined by USEPA (10th percentile) = 12%. ,"
Upper PMSD bound determined by USEPA (90th percentile) = 30%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratmy Variability Study (USEPA, 2001 a; USEPA, 2001 b).
USEPA 2001a, 2001b. Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-0OI-005. US Environmental Protection Agency, Cincinnati, OH.
"ETS SEnvtronental TetingS Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 11/17/2009 Test ID: PpKCICR Sample ID: REF-RefToxicant End Date: 11124/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments:
Cooc-mg/L 1 2 3 4 D-Control 1.0000 1:0000 1.0000 0.9000 450 0.9000 1.0000 1.0000" 1.0000 600 0.9000 1.0000 1.0000 0.9000 750 0.8000 0.7000 0.900 0.7000 900 0.4000 *0.5000 0.4000 0.4000 1050 0.2000 0.1000 02000 0.2000 Transformu Arcsin Square Root I-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Resp Number D-Cohtrol 0.9750 '1.0000 1.3713 1.2490 1.4120 5.942 4 1" 40 450 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4 0.000 2.410 0.1471 1 " 40 600 0.9500 0.9744 1.3305 1.2490 1.4120 7.072 4 0.668 2.410 0.1471 2 40
- 750 0.7750 .0.7949 1.0846 0.9912 1.2490 11.294 4 4.696 . 2.410 0.1471 '9 40
- 900 0.4250 0.4359 0.7099 0.6847 0.7854 7.091 4. 10.836 2.410 0.1471 23 40
'1050 0.1750 0.1795 0.4282 0.3218 0.4636 16.570 4 15.451 2.410 0.1471 33 40 Auxiliary Tests . Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.91163939 0.884 -0.1384496t -0.6290117 Bartlett's Test indicates equal variances (p = 0.82) 2.20082235 15.0862722 Hypothesis Test (l-tail, 0:05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df
.Dwmetfs Test 600 750 670.820393 0.07612662 0.07923945 0.63093674 0.00745088 7.3E-12 5,18 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slone 1190075 202798845 7.9258926 15.8756075 0.025 0.02529665 7.81472778 I 2.94271355 0.08402832 3 Intercept -30.020498 5.96965262 -41.721018 -18.319979 TSCR 0.02489373 0.0167698 -0.0079751 0.05776254 Point Probits mg/L 95% Fiducial Limits ECOI 2.674 558.771764 .439.30398 632.540335 EC05 3.355 637.528005 533.944092 700.273975 ECI0 3:718 683.954056 591.864566 740.053467 ECI5 3.964 717.172783 633.972402 768.743169 EC20 4.158 744.720889 669.099597 792.886858 EC25 4.326 769.196521 700.275338 814.793373 EC40 4.747 834.497847 781.392728 877.222284 ECSO 5.000 876.422566 829.69787 922.532758 EC60 5.253 920.453569 875.571987 976.186221 EC75 5.674 998.595923 946.148753 1085.24501 EC80 5.842 1031.4153 973.075482 1134.89329 EC85 6.036 1071.03413 1004.29166 1196.99431 EC90 6.282 1123.05276 1043.78666 1281.46378 EC95 6.645 1204.83569 1103.60002 1419.80285 EC99 7.326 1374.65171 1222.28693 1724.95223 Organisms obtainedfrom Aquatox, Inc. atoxlI 1- 17-09
I,........,
Statistical Analyses Environmental Testing Solutlons, Inc.
Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/17/2009 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 11/24/2009 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas -
Comments:
Conc-mg/L 1 2 3 4 D-Control 0.9070 0.8050 0.8430 0.7440 450 0.7030 0.7590 0,8540 0.8160, 600 0.7790 0.8480 0.7150 0.7890.
750 0.6840 0.6310 0,8590 0.6190 900 0.4330 0.4250 0.3200 0.3340 1050 0.1550 0.1180 0,1530 0.1710" Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N, t-Stat Critical . MSD Mean N-Mean D-Control 0.8248 1.0000 0.8248 0.7440 0.9070 8.285 4 . 0.8248 1:0000 450 0.7830 0.9494 0.7830 ,0.7030. 0.8540 8.441 4 0.933 2.180 0.0975 0.7830 0.9494 600 0.7828 0.9491 0,7828 0.7150 0.8480 6.959 4 0.939 2.180 0.0975 . 0.7828 0.9491 750 0.6983 0.8466 0.6983 0.6190 '0.8590 15.872 4 .0.6983 0.8466 900 0.3780 0.4583 0.3780 0.3200 0.4330 15.676 4 0.3780 0.4583 1050 0.1493 0.1810 0.1493 0.1180 0.1710 14.966 4 .- 0.1493 , 0.1810 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93505901 0.805 ., -0.079593823 -1.15766298 Bartlett's Test indicates equal variances (p = 0.93) 0.14925823 9.2103405 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600 >600 0.09751355 0.11823407 0.002338083 0.00400172 0.57733494 . 2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL(Exp) Skew IC05* 444.48 171.45 43.33 - .936:54 -0.1280 ICI0 671.85 93.51 216.91 825.11 -1.2941 IC15 745.05 . 48.64 537.03 808.42 -0.9607 IC20 768.01 29.70 646.35 ,819.59 -1.0239 IC25 787.32 20.62 712.96 835.30 -0.5410 IC40 845.27 13.72 797.28 882.88 -0.0132 IC50 883.90 13.24 846.50 930.45 0.3616
- indicates IC estimate less than the lowest concentration Organisms obtainedftom Aquatox, Inc. atoxil1-17-09
Page 4 of 5 EnVImnanmUIT..iIn, Sokntto.,j Inc.
Species: PimephalesDromelas PpKCICR Test Number: ____
Daily Chemistry:
Day Concentration Pa'rame'ter
" PH (S.U.) -4.qe I 74 7 Ia '7.SQ C.q DO (mg/L)
Conductivity (Amho/cm) 3 S35.328 CONTROL Alkalinity (mg CaCO 3/L) A Hardness -
(mg CaCO3 L)
Temperature pHi (s.u.) . ., - r'l, 450AmgKCI/L' 110 "1*luO I[00 450 mg KCIL Conductivity Temperature -LA.
(c)nd 600 mg KCI/L Conductivity y (Amhos/cm) ~ S(
Temperature DO (s.) I.4
.750 mg KCI/L Conductivity .O 1090 1o Temperature " .
(-c) pH (S.U.)
F IIjs1 II -
- ,510 1 '41m' HI.-I.q I -1.1C, gI4 I DO (m") "'4 7.3 11.8 1 4.
Conductivity 900 mg KCVL (ji.mhos/cm)
Temperature 14 U-'L LA.W (0C) pH (S.U.) I f4iýp I 'I.I.Q q 7-44 1 'mot. -11 Q.4-7,0 - I DO (mgf/L) I-Conductivity 1050 mg KC/L (umhos/cm)
Temperature 19 0
(°C,) 14-8 OL4.e . -zs.
.2q.j.. , 00(i z4-4 zq.,f STOCK rConductivity G O0 I (Rdhslm Initial I I Page89 of 102 SOP AT21 - Exhibit AT2 1.1, revis
Page 5 of-5 S
EA~fr~.n,.nt.IT.u1ng Species: Pimephalespromelas PpKCICR Test Number: J I Dayv I Do I
....._______ Analyster ~- t..~
Concentra'tio~n aamt IpH (S.U.) JM7 -I.GS -4.4a I DO (mJ/L) "U Ile Conductivity (ramhos/cm) -334 Alkalinity CONTROL (me CaCO /L)
Hardness *
(mg CaCO 3/L)
Temperature (0C) ILA. -LA .1 ILA k vo, vk I I- I[ I III ---
pH (S.U.) /ý-80 II -7.5q 1 UA~ 11 13 11 1~ q,4 1z'110o 1 Conductivity 450 mg KCI/L (Tmhospcm) 0-L #1~ _ .6 7-L Temperature (0c)
PH (S.U.) 1UO 7,1
".12 ,I -. "."" " :l-600 mg KCVL Conductivity - q "1. l e . VA .k -&" ,
- Temperature
-4.
(0c)
(Rtmho /M) 75 0 mg KCI/L inhos/cm0 l .I,£* O 1 00l q Temperature -D .'4 Io. ILA. rII .r
~ mgKCVL Conductivity PH (S.U )
- 7.Le 1.LP-1 "-., IP , -3 ':.'3 - 1*e00 :.~
1050 mg KCI/L Conductivity (jimhos/cm) 21(O2C) I '
(Q
- -(.U1-76 --
2q -4 -)
(0c),
Temperature -L,. ,- vk.l
-4 Initial Final Initial Final Initial Final Initial Final
... '. ;:SOP AT21 - Exhibit AT2 1.1, revision 04-01-09 Page 90 of.102
Ceriodaphniadubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.
I.." I I I I I I I I I I I I I ! I l" I I USEPA Control Limits (+2 StandardDeviations) 1.12 1.08 ..
1.06 1.04 - _ I I 1.02 2.5 I I I " I I I I l I l I I. I l I l I I , I USEPA Warning and ControlLimits ( 75 th and 9 0 th PercentileCVs)
Q 2.0 1.5 Jn 1.0 IU 0.5 ............................
+ I I I .I I I 1.4 I I ' I I I i !I I I I II I I I I I I 1.3 Laboratory Warning and ControlLimits (10 th and 251h Percentile CVs)
.......................... ... I..................
1.2
. I****------------- ------ -------
1.1
- -o - -
1.0 0.9 0OR 08v Test date
7-day IC2 s = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- -- Central Tendency (mean lC 25 )
Warning Limits (mean IC25 SA.10 or SA.75)
....... aCet iits (mean 1C25 + SA.25'SA.90, or 2 Standard Deviations)
1ETSEnvironmental Testing Solutions, lncý State and USEPA Ceriodaphniadubia Chronic Reference Toxicant Control Chart Laboratory Laboratory- USEPA". . USEPA -
Test number Test date 7-day JC' CT S, Control Limits SA." Warning Limits SAns Control Limits SL75 Warning Limits. ,S Control Limits Cv, 5
(g/L NaCI) (g/L NaCI) CT-2S CT+2S CT - SA 0o . CT + SA0o CT - SA.25 CT + SAm CT - SA.75 CFt Sý . CT - Sk.o CT + SA" 1 11-04-08 1.07 2 11-04-08 1.10 1.08
- 0.02 1.05 1.12 0.09 1.00 1.17 0.18 . 0.90 1.27 0.49 0.60 1 571 0.67 0.41 1.75 0.02 3 12-02-08 1.06 1.08 0.02 1.04 I.iI 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 156 0.67 0.41 1.74 0.02 4 01-06-09 1:05 1.07 0.02 1.03 1.I1 0.09 098 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 *0.02 0.02
- 5. 02-03-09 1.05 1.07 -0.02 1.03 .1.10 0.09 0.98 1.15. 0.18 0.88 1.25 0.48 0.59 1,55 0.66 0.41 1.73
.1-07 0.02 1.03 0.02 6 02-24-09 1.07 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 155, 0.66 041 1.73 7 03-10-09 1.07 1.07 0.02 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 155 066 0.41 1.73 0.01 8 04-14-09 1.09 1.07 0.02 1.04 1.10 0.09. 0.98 1.16 0.18 0.89 1.25 0.48 0.59 155- 0.66 0.41 1.73 9 05-05-09 1.07 1.07 0.02 1.04 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01
- 10 05-05-09 1.08 1'07 0.02 1.04 1.10 '0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 S11 06-09-09 1.07 1.07 0.01- 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 12 06-09-09 1.07 L.07 0.01 1.04 1.10 0.09 0.99' 1.16 0.18 0.89 1.25 0.48 0.59 1.55;'" 0.66 0.41 1.74 0.01 13 06-18-09. 1.06 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 155 0.66 0.41 1.73 0.01 14 07-07-09 1.06 1.07 0.01 1.04 .10 0.09 0.98 1.16 0.18 0.89, 1.25 0.48 0.59 155*:ý 0.66 0.41 1.73 0.01 is 08-04-09 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 -0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 16 08-04-09 1.09 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.01 17 09-15-09 1.05 1.07 0.01 1.04 1.10 0.09 0.98 1.16 0.18' 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 18 10-06-09 1.08 1.07 0.01 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1L55-' 0.66 0.41 1.73 0.01 19 10-14-09 1.07 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0,41 1.73 1.74 0.01 20 11-10-09 1.07 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 041 Note: 7-d IC25 day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT - Central tendency (mean IC25 ).
S - Standard deviation of the IC-5 values.
Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation ofrthe ICs values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.
S.1o = Standard deviation corresponding to the l0i percentile CV. (Skio = 008)
Sas = Standard deviation corresponding to the 2P percentile CV. (SAZs = 0.17)
USEPA Control and Warning Uimits S'Is = Standard deviation corresponding to the 75P percentile CV. (SA~s = 0.45)
SA..0 = Standard deviation corresponding to the 90eh percentile CV. (SAgo = 0.62)
CV Coefficient of variation of the IC2s values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Precision of Endpoint Measurements Ceriodaphniadubia Chronic Reference Toxicant Data I- Environmental Testing Solutions, Inc.
"Test. *Tes da -Control, Control Mean'.
uestr ate . Survival....
number,............ ... Rep.routn 0.Keproducuion . .. ,-CT
.. - Cv CT. -' MSD PMSD CT for Control Mean for Control
(%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%)
(offspring/female) CV (%)
.1 11-04-08 100 32.3 5.1 2.2 6.8 2 11-04-08 100 31.5 31.9 6.2 5.6 2.4 7.7 7.2 3 12-02-08 100 31.5 31.8 4.6 5.3 2.5 8.0 7.5 4 01-06-09 100 33.2 32.1 4.0 4.9 2.0 6.1 7.1 5 02-03-09 100 31.4 32.0 6.6 5.3 1.9 6.0 6.9 6 02-24-09 100 32.2 32.0 6.0 5.4 1.8 5.7 6.7
-7 03-10-09 100 31.9 32.0 5.8 5.5 2.6 8.2' 6.9 8 04-14-09 100 33.9, '32.2 6.7 5.6 2.0 5.8 6.8 9 05-05-09 100. 33.6 32.4 4.3 5.5 2.3 6.8 6.8 10 05-05-09 100 34.6 32.6 7.7 5.7 2.2. 6.5 6.8 11 06-09-09 100 31.3 32.5 6.4 5.8 2.1 6.7 6.7 12 06-09-09 100 31.1 32.4 9.4 6.1 2.6 8.3 6.9 13 06-18-09 100 32.3 32.4 7.3 6.2 1.9 5.8 6.8 14 07-07-09 100 29.9 32.2 3.3 .6.0. 2.2 7.2 6.8 15 08-04-09 100 30.4 32.1 4.7 5.9 2.2 7.3 '6.9 16 08-04-09 100 32.0 32.1 5.1 5.8 2.2 6.9 6.9 17 09-15-09 100 31.6 32.0 5.4 5.8 2.2 7.0 .6.9 18 10-06-09 100 31.4 32.0 4.3 5.7 2.4 7.6 6.9 19 10-14-09 100 33.3 32.1 3.5 5.6 2.6 7.7 7.0 20 11-10-09 100 34.0 32.2 6.2 5.6 2.6 7.7 7.0 Note: CV = Coefficient of variation for control reproduction.
Lower CV bound determined by USEPA (10th percentile) = 8.9%.
Upper CV bound determined by-USEPA (90t" percentile) =42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the:control and treatment that can be declared statistically significant in a whole effluent toxicity test.
Lower PMSD bound determined by USEPA (I0th percentile) = 13%.
Upper PMSD bound determined by USEPA (90th percentile) = 47%.
CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01l-005. US Environmental Protection Agency, Cincinnati, OH..
Page 93 of 102.g 93 of ,. 1-10-09.
Ceriodaphniadubia Chronic Reference Toxicant Control Chart 0"ETS Precision of Endpoint Measurements
,*]'i -. Environmental Testing Solutlorns, Inc.
40 .I I *I I I I I .1 I I I I I I I* I I I I -
35 .. . . . . . . . . 4 . . . . . . . 0 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
,* -C S20 I '-;9. 115 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
I I ' I I I I I I I I I I I I I II I -
g .40 L I I I . I I I I I I I I I I I I I I I I I North Carolina Acceptance Limit (< 40.0%)
4f,I "30 20 Kentucky Acceptance Limit (<30.0%)
10 420 I I I I I I -I I I I .I I.I....I...
5.0 . I I I I I I I I . I I . I I I I I
. USEPA Upper PMSD Bound (90 thpercentile < 47.0%)
40 r0) 30 20 10 S. . ............ ...... ..
A3 v
Test date
- Control Reproduction, Coefficient of Variation (CV),.or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- -"Central Tendency (mean Control Reproduction, CV, or PMSD)
..... pa4l* */Anits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Ir l:T
- ':ET~t m Sl'la dtLl¢ Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia CdNaCICR #: I-00 Dilution preparationinformation: Comments:
NaCI Stock INSS number: l r4*, "_. _ _ _ _ _
Stock preparation: 100 g NaCI/L:
Dissolve 50 g NaCI in 500 mLMilli-Q water.
D~lution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 " 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 1 Test organismsource information: Test information:
Organism e: I< 24-hours old Randomizing template color:
Dateand times organisms were born i,-"b-O% 0l.W'r IDO Incubator number and shelf between: location:
2.6 7-.-
cultureboard: --
Replicate number: 1 2 3 4 1 6 7 9 9 10 YWT batch:
Culture boardcup number: 1% k I i 1 W_1&-&EI v1*Of 1.
Transfer vessel information: pH = _'. S.U. Temperature = 1. °C Selenastrum batch: I l-0I oi Average transfer volume (mL): 0. 0 51s ._ __... .
Daily renewal information:
Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0i 0 I- 1 1otto I t-01--01 A ;2)G4a 2 IlV-1o' __________ l-01-' A 24 102*OA lo-tst- A 5
___ ii- r-o' i J Controlinformation: _ _ Acceptance criteria Summary of test endpoints:
%_ofMaleAdults: s 20% 7-day LC50 - > bU
% Adults having 31d Broods: I
______, a 80% NOEC &t
% Mortality: O7. s_200/6 LOEC i to Mean Offspring/Female: 34 o> 15.0 offspring/female ChV 0/. CV: , ,, -7.1 <40.0% l.C_ _ ._ 10-12L., I Page 95 of 102 SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09
I FT*
Page 2 of 6 Species:- Ceriodaphniadubia aCICR U:100 CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 A-:*
Young produced - - - Q- . *hh I I) I
.2 -
Adult mortality
!. Young produced (C) t.- . )
L
) *
._.s .. *.'.
\_
g,'i Adult-mortality t- t (C) 3 Young produced 0Q p 0 P 0 I" C C Adult mortality . t * '._ , \ ,_. ' 1-.... '
4 "Youngproduced " . ! l Adult mortality i \.
- L. _ .. _
5 Young produced V5, to 5L I4 [L ' I Adult mortality _.., -.
6 .Young produced 0 j 0 A It ' l IL 0 V--.
Adult mortality * . [ % . .
7 Young produced I %to " --I Ile I Total young produced 56. 3% 3\. 35 36. .%- '3:S 3**3\
Final Adult Mortality W - . *W * - _ % . ' *?
Xfor 3' Broods "7- 1 5 -1 Note: Adult mortality (L = live, D dead), SB split brood (single brood split between two days), CO = carry over (offspring q carried over with adult during transfer).
Concentration:
. % Mortality: o7.
C Mean Offspring/Female: Z4'. 0 600 -mg NaCI/L Survival and Reproduction Data Replicate number _
Day . 2 3 "4 *5 -6 7 8. 9 10 1I Young produced C) C - "-
I"Adult mortality .- .. . 7---
- 2 Young produced 0C , C Adult mortality 11L. ,
3 Young produced (5-7 o ) "...Q0 -c0 - )
Adult mortality i_ L_ '._ L L L.._ " _
Adult mortality --
43 Young produced 0=
5 Young produced \ * . .4i ŽL. S S I Adult mortality \.. t. I.. L... .. L ... -.
6 Young produced 75 0 (3 (3) 0 -
I Adult mortality , [ L L.%
t__ L.L- L L -
7 Young produced 14ý Ile l V) U '.1 Total youngproduced "
- I s 3 . j ,* 3S 30 Final Adult Mortality.. L . T t. C. T? . K.. .
Note: Adult mortality (L = live, D dead), SB = split brood (single brood split between two days), CO = carry over (offspring earried over with adult duirine tranqf'er*
carried over with adult durina transfer)
Concentration:
%Mortality: 07.
Mean Offspring/Femate: -*. 0
% Reduction from Control: 0 7.
Page 96 of 102 SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09
Page 3 of 6
,*S Species: Ceriodaphniadubia CdNaCICR #:- tO0. - 4.;!
..800 mg NaCI/L , Survival and ReDroduction Data Replicate number
-Day .. 1.2 3 4 5 6 7 8 9 10
- .,. YVoungproduced .C -*0. C C'(I 0 n 0 Adult mortality .. * - - .
. .Young produced.,.: * ) C5ft r) ) C) 0-7 Adult' mortality - ,,. C. * ". t, '
3 Young produced -CL [ C O Ž C C)
Adult mortality . . ,. .. .. .
- L.1 -,
4 Young produced S .4 , -
Adult mortality L-
- ____
- t s Young produced Oil-. , L 10 1 IL. %.
I It. L 'L
.. I--
Adult mortality \ \. - \- v L \-- %,.-
6 Young produced '= . F) E (5
_ Adult mortality L-.- . I.._." ,. . U I- -
Young produced l"A 4 14 !
Total young produced bk 5A ZV a 3A~ 31 35 3$ 3%
Final Adult Mortality .-
Nole: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).
Concentration:
% Mortality: (V.
Mean Offspring/Female: I *. (5 0%Reduction from Control: A. Al.
1000 mg-NaCI/L Survival andReproduction Data
..... Replicate number Day Young produced V
Adult mortality 1
I 2
_ _ 3 4
-7 5
6 L.
(O 7
i ..
8 L.
C 9
C),,
10 2 Young produced C) (0) 0 - . ,
Adult'mortality \. ..- - T7 - ITT \.... %.-
U L.-.-
3 'Young produced ID C Adult mortality - . U .. " U U ... U U 4 Young produced _l . _ 14, Adult mortality L . U. U.. U_ U U -
5 Young produced . 9 I1. 1" 10 l0O / '3 Adult mortality * .1. U U .. C--
6- Young produced "0 C6 I Adult mortality L- , L L L L. u LU "
7 Young produced, v'S- - - -1 A 1- 14 Total young produced .3 [30 ,31 31 , "
I Final Adult Mortality ".
Note: Adult mortality (L = live, D = dead). SB carried over with adult during transfer).
split brood (single brood split between two days), CO = carry over (offspring Concentration:
% Mortality: 0.
Mean Offspring/Female: 30.'
% Reduction from Control: .106,1.7 Page 9 7 o.f 102 .SOP AT14 - Exhibit AT14.1, revision 04-01-09
Page 4 of 6 EwamwatgmlbnfrnScjbeh.,a
. Species:,
. Ceriodaphniadubia c: .CICR# .
-1 2O00'iri aCIIL Survival and Renroduction Data
-... ..... Replicate number -.
Day .... 1 2 3 4 5 6 7. 8_ 9. 10
- . Youn gp I rodueed I 0 0
"" ""l"Adult mortality _ * . _ -
.... 9ig.dult pr"A cctiity C,.. n_ .. .. k..
- k,. k.
3 -Young produced 0 75 " ) 0 Adult mortality U- U U U U - U -
4 Young produced _ _ 0 0 Z.
- O.T *-
Adult mortality U.- U I,." .. ,,
5 Young produced = 1 Adult mortality *, ,_ ,,. ,.. I... ._
6 Young produced .) 0 0 0 0 Adult mortality U .. . U Q_ i U . U-.U-'.
7 Young produced - - 44
- 0% -..-
Tot al young produced d - -'1 0 'l,1 -1 -Lo -t Final Adult Mortality \. .. U U U . - . . U Note: Adult mortality (L = live, D =dead), SB =split brood (single brood split between two days), CO --carry over (offspring carried over with a duce transfer). )uring Concentration:
% Mortality: U- U .
Mean Offspring/Female:
't .16.
...... %Reduction from Control: *.50 7 1400 mg NaCI/L Survival and Reproduction Data Replicate number Day, 1 1 2 3 1 4 5 6 7. 9 9 10 I Young produced -) - - __
Adult mortality ,. ",. .. * .[,. L I_,. tI,_
2 Young produced O )
- O "o zo I* O1 O -
Adult mortalitya--
f---- U U_ - \. ,-. I,__ k..,
3 Young produced " ) O OC) C) O C* "
Adult mortality (ilbosi bwt d CO r orfi 4 Young produced o T 0I0 Adult mortality 5 Young produced cn fo C Adult mortalityL Surviva. a Rrui Data 6 Young produced Adult mortality L._ LL U- U- U-i 7 Young produced FnlAdult Mortality Total young produced i-C-
I K.-
I1..
L U
- U Note: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), tCO = carry over (onspring carried over with adult during transfer). .___ _.
- 0 Concentration:
% Mortality: 7.
-.Mean Offspring/Female: ".,°
% Reduction from Control: .1,& 7-Page 98 of 102 SOP AT 14 - Exhibit AT 14.1, revision. 04-01-09
Ceriodaphniadubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control
- Environmental Testing Solutions, Inc.
Verification of Data Entry, Calculations, and Statistical Analyses Test num*ber: CdNaCICR #100 Test damtes: November 10-17, 2009 Reveiwed by:
Concentration Replicate number Survival Average reproduction coefficient of Percent reduction from (mg/L NaCI) 12 3 4 5 6 7 8 9 10 (%) (offspring/femaie) varation (%) control (%)
Control 36 31 36 33 36 34 35 32 36 31 100 34.0 6.2 Not applicable 600 33 38. 33 ,35 36 33 35 32 35 30 100 34.0.. .6.6 0.0 800 34 .34 34 36 31 31 33 35 31 31 100 33.0 5.7 2.9 1000 29 33 30 29 30 31 31 29 28 34 100 30.4 6.2 10.6 1200 20 14 17 10 20 20 13 17 .20 18 100 16.9 20.8 50.3 1400 9 7 9 2 7 12 10 5 6 12 100 7.9 ." 39.8 76.8 Dunnett's MSD value:. 2.604 MSD = Minimum Significant Difference PMSD: 7.7 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatmret that can be declared statistically significant in a whole effluent toxicity test.,
Lower PMSD bound determined by USEPA (10t percentle) 13%.
Upper PMSD bound determined by USEPA (90&' percentile) = 47%.
Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Inierlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).
USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA.-2-B-01-005.
US Environmental Protection Agency, Cincinnati, OH. I
- ,.4. A EFTS:
%ýEnvl~mental TeOt)V SoqfWui4 Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: .11/10/2009 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date:. 11/17/2009 Lab ID: ETS-Envir. Testing Sol0 Sample Type: NACL-Sodium chloride*:
Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia,'
Comments:
Conc-mg/L 1 2 3 4 5 *6 7 8 9 10 -
D-Control 36.000 31.000 36.000 33.000 36.000 34.000 35.000 32.000 36.000 . 31.000
.600 33.000 38.000 33.000 35.000 36.000 33.000 .35.000 32.000 35.000 30.6000 800 34.000 34.000 34.000 36.000 31.000 31.000 33.000 35.000 31.000 31.000 1000 29.000 33.000 30.000 29.000 30.000 31.000 31.000 29.000 28.000 34.000 1200 20.000 14.000 17.000 10.000 20.000 20.000 13.000 17.000 20.000 18.000 1400 9.000 7.000 9.000 2.000 7.000 12.000 10.000 5.000 6.000 12.000 -
Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 34.000 1.0000 34.000 31.000 36.000 6.201 10 34.000 34 1.0000 600 34.000 . 1.0000 34.000 30.006 38.000 6.649 10 0.000 2.287 2.604 34.000 ,1.0000 800 33.000 0.9706. 33.000 31.000 36.000 5.714 10 0.878 2.287 2.604 33.000 0.9706
- 1000 30.400 0.8941 30.400 28.000 34.000 6.241 10 3.161 2.287 2.604 30.400 0.8941
- 1200 16.900 0.4971 16.900 10.000 20.000 .20.771 10 15.015 2.287 2.604 16.900 0.4971
- 1400 7.900 0.2324 7.900 2.000 12.000 39.783 10 22.917 2.287 .2.604 7.900 0.2324 Auxiliary Tests . . Statistic Critical Skew Kurt 0 0 Kolmogorov D Test indicates normal distribution (p > . 1) 0.85725808 1.035 -0.4383847 0.05843284 Bartlett's Test indicates equal variances (p = 0.27) 6.40927744 15.0862722 Hypothesis Test (]-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE . F-Prob .
- df Dunnett's Test 800 1000 894.427191 2.60422823 0.07659495 1213.54667 6.48518519 8.2E-33 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples)
Point mg/L- SD 95% CL Skew IC05 853.846154 68.891583 658.291667 950.259615 -0.9158 -
ICIO 984.615385 37.1605902 889.984375 1018.01841 -0.4734 I.
IC15 1022.22222 10.1708642 .1001.41628 1040.13001 0.1120 IC20 1047.40741 9.4426387 1027.71794 1065.06914 0.2104 IC25 1072.59259^9.34744229 1054.19332 1087.34371 0.2270 IC40 1148.14815 11.8528783 1126.04654 1168.22075 0.4312 IC50 1198.51852 16.0574224 1169.42431 1229.62477 0.4948 11-10-09
lET Page 5 of 6
-. Species: Ceriodaphniadubia CdNaCICR #1 P00 Daily Chemistry: _ _ _ _ _ _ _ __ _ _ _ _ _ _ _
I B
Day I 0 I I I 0-
-Analyst
- i
~A;w !
I II I-A,
'-A Concentration Parameter m
PH (s.u.)
DO (rag/L)
Conductivity (Amhos/cm)
CONTROL Alkalinity (mg CaCO3/L)
Hardness (mg CaCO 3/L)
Temperature (0C) w - S U - U- U - n-1H (S.U.) - -
DO (mtJL) 4-600 mg NaCI/L Conductivity 1441O :1 S3-(umhos/cm) 4-Temperature pH (S.U.) -T q~~,q q -~~q 144(
747 11 DO (mz/L)
(0- q. q Conductivity 800 mg NaCI/L
(*mhos/cm)_
Temperature (0c)
U l
U - - w -. Ir pH (S.UX) 5. 4-01 4,814' '4- W -1 DO (rag/L) 4 ... 22.
1000 mg NaCVL. Conductivity (A hos/cm)
Temperature (TC).
1200 mg NaCI/L Conductivity
-'..hosTmp Z*30 TemperatureL '2114A. ,4 ',OzA1 1A
_ _ -c) nHl (S.AJ.' ':~~3
~ ~ '4t.
q q B'3 I q I 'I~4e IP~q PH (SU).
DO (m2/L)
Conductivity.
1400.mg NaCV/L (gmhos/cm)
Temperature -7 (C) u STOCK , Conductivity M JMM (gmhoskcm ) I. ll Initial F I Initial II Final I Initial I -Final I Page 101 of 102 SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09
r' Page 6 of 6 Species: -Ceriodaphniadubia CdNaCiCR #: r Day I - :-.1 .S 3 :-., - - I- 4 5 6
....... Analyst- II 9.- It-M
, -^2 ,T V II 14J!11 concentration Parameter pH.S.U.. 1 .50 T.7 3. -
DO_(mg1. 1.9 E3 Conductivity (WMxnOSlCM) JLID CONTROL Alkalinity (mg CaCO 3/L)
Hardness (mg CaCO 3 /L) 901 Temperature ~b ~~ ~ 1..~
(0c) - -7 H S.U 7 .ý 600 mg NaCIIL Conductivity
~ §33 (imhosI m)
Temperature"IL, ,
- o(T) , .,orq*, Te .o * , ta* j 0t*
r pH (S.U.)
- llal,ý
~ 41-'-~
-1.-14 I.......-
111L 11.1qR 4I~ II.- 14I61 11 .4~1Z I DO (mg/L) 800 mg NaCl/L Conductivity1 (gimhos/cm) j.I AA- .1 7. - . 0
'Ii (Temperature 0
c) . -
m* I ii l m IWOm-m I
pH (S.U.) * ,I 0 I-H -
.~~
I 4
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IIII -~.*~---
I -4 0 -. ~-~---
iiI I '~pf -
1-o? 1 DO (reg./L)
- 1000 .mg NaCI/L Conductivity
ý32520 z3C0 2 530 (pgmhoslcm)I Temperature I*L I (0C)
- I PDO 1(mg/L) .t 7.5-6 .2.
1 1.200 mg NaCI/L Conductivity Z090 2Temperature 1Temperaurhos/M) p~~~H(S.U.). 14-BL
"-.* .. .5 2--".1,,
1q u4.0 11 .1
" .X M-44 I
- ,*] :
PH (S.U.) PAj 7 -7~ uS '113 1400 mg NaCI/L Conductivity (utmhoslcm)
~4= ail 312.0;Y 31'40 Temperature (0C)
..tL:u*L
-U I-omn aI l
- 8 I
~ h ____
I Initial imI I Final i *n Initial Final I Initial nn Final -
Initial j[
i Final Im I. Page 102 of 102 SOP ATI4 - Exhibit ATI4t.1, revision 04-01-09
..i; PERMiTTEE NAMEIADDRESS (OcludeFacilty Name/Location ifDifferent) * . NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR ,' Form/ \pproved.
TVA - SEOUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Name (SUBR 01) OMB 0o.2040-0004 Address P.O. BOX.2000 -
(INTEROFFICE.SB-2A-SCN) ... 6450 1 F- FINAL ' -
SODDY - DAISY. TN 37384 I PERMIT NUMBER EDISCAR NUMBER LOW VOL. WASTE TREATMENT POND-Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio , HAMILTON COUNTY ME NITORING PERIOI EFFLUENT From YEARP I( LnAY--
"n YIA
ý12 mnC n AY
-Froml 09 1,12 F 017 TO 1 9 12 1 31 **" NO DISCHARGE GE Z*
ATTN: Stephanie A. Howard NOTE: Read instructions before comoletinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR-CONCENTRATION: NO.ý FREQUENCY SAMPLE
- EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM. UNITS ANALYSIS PH SAMPLE .. ** 7.2 82 12, 0 13/31 GRAB MEASUREMENT 8 0 00400 1 0 0 . PERMT
- 6.0 90* T HREE GRiA EFFLUENT GROSS VALUE MINIMUM MAXIMUM W.............EEKGRAB SOLIDS, TOTAL SUSPENDED SAMPLE 56 81 26 6 88 19 19-,
0 5 / 31 GRAB MEASUREMENT 00530, 1 0 0 PERMIT ~< 380 1250 LBS/DY * **71'AQ.00GLWEKY GRAB EFFLUENT GROSS VALUE REIMETMOAVG DAILYMXREQUREMEN EFFLUENT VALUEMO GOSS AVG DAILY MXMOAG < **** <6 DIY6 X: ii9 0 5 /31 GRAB OIL AND GREASE SAMPLE <56 <60 26 6<6 195 MEASUREMENT 00556'- 1 0 0 . PERMIT < 190 250 LBS/D`Y 15 ' 20 MGIL WEEKLY GRAB EFFLUENT GROSS VALUE MO AVG DAILY MX MO AVG DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 1.196 1.446 03
- 0 31 / 31 TOTALZ TREATMENT PLANT MEASUREMENT 50050. 1 0 :-0 REQIRMET REPORT EFFLUENT GRXSS VALUE MO A DI MXERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT 5PERMIT, SAMPLE MEASUREMENT PERMIT.K REQUIREMENT NA ETJL PI4CPL XCUIEOFFICER IlCertify under penalty of law that this document and all attachments were prepared under my TEEPON DAT PRINCIPAL EXECUTIVEdirection or supervision in accordance with a system designed to assure that qualified personnel NAME/TiTLE (~~k.§x A TLPOEDT EP om3301(E Seuya it 3/9)
Christopher R. Church ie rsiet Pevous ditons ma be usedi properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the intormation, the information submitted is ,to the best of myknowledge and belief, true, Environmental Manager SION & WBN 43 01 Page 1of~ii !ii~
1 423_____________________10___01__07_
1 0 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED ORPRINTEDinformation. includingthe possibility of finý and irmprisonmenttfor knowing violations. OFCRRAUORZDGET .AREA NUMBER. YEA MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he, EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of I
PERMITTEE NAME/ADD6RESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
TVA - SEOUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT . (DMR) OMB No. 2040-0004 Name (SUBR 0.1)
Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)
SODDY - DAISY. TN 37384 TZI026450 ll 107 G F -FNAL METAL CLEANING WASTE POND
- PERMIT NUMBER LDISCHARGE NUMBERI FacilitY TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY EFFLUENT SFrom l0 r YoARmMO - 1 I011 Tol 09 12 3 DAY" Y DAYTo NO DISCHARGE D .
ATrN: Stephanie A. Howard NOTE: Read instructions before completinq this form, PARAMETER
><-I AVERAGE QUANTITY OR LOADING MAXIMUM - UNITS MINIMUM QUALITY OR CONCENTRATION AVERAGE MAXIMUM - UNITS NO.
EX FREQUENCY OF ANALYSIS SAMPLE TYPE PH SAMPLE 8.0 8.7 0 7/31 GRAB MEASUREMENT 12 I..
00400 1 0 0 PERMIT * * * **** * * * ** * **
- 6.0 9.0 SU D--AILY GRAB REQUIREMENJT EFFLUENT GROSS VALUE MAiVIAian SOLIDS, TOTAL SUSPENDED SAMPLE 2 *19 0 7 / 31 COMPOS MEASUREMENT 19 00530 1 0 0 PERMIT ************* '.Y. 30 FMGGL1L ... DAILY . 'OMPOS.
EFFLUENT GROSS VALUE REQUIREMENT DAILY MX-OIL AND GREASE SAMPLE ******** ******** ** ******** <6 731 GRAB MEASUREMENT 1*. 0 00556 1 0 0 . .PERMIT . **** 44 ~~**1 GL~ AL~GA EFFLUENT GROSS VALUE REQUIREMENT . ' DAILY MX _____. _ _
PHOSPHORUS, TOTAL (AS P) SAMPLE **....... **...... ** *......0 19 MEASUREMENT 00665 1 0 0 PERMIT'MG/L 1.0 DAILY* MPOS EFFLUENT GROSS VALUE REOIRt~(ENT DAI Y Mx ___.__.
COPPER, TOTAL (AS CU) SAMPLE 0.**023 19. 0 7 / 31 COMPOS MEASUREMENT
'KPERMIT>' MG/ DAILY OMP*
01042 1 0 0 ,*'.0**
EFFLUENT GROSS VALUE . REQUIREMENT~ DAL M..X GI ALYCM IRON, TOTAL (AS FE) SAMPLE ********* ***0. 14 1'9 0 7 / 31 COMPOS MEASUREMENT 01045 1 0 0 PE RMITK K> **** **** **** 1.0 MGIL DAILY ICOMPOS EFFLUENT GROSS VALUE REQUIREMENT .' . . . . .DAILY MX' . ....
FLOW, IN CONDUIT OR THRU SAMPLE 0.021 .0.034. 03 . 0 7/31 CALCTD TREATMENT PLANT MEASUREMENT 50050 1 0 0 PEýRMIT -- REPORT REPORT MGD ~ r#DAILY CALCTD
,EFFLUENT GROSS VALUE [REQ()UIREME NT LMO AVG DAILY MX _______ ________
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my , it .-. i TELEPHONE DATE
"-irection or supervision in accordance with a system designed to assure that qualified personnel . /
Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons.directly responsible for gathering the Environmental Manager SQN & WBN 423 . 843-7001' 10 01 07 information, the information submitted is to the best of my knowledge and belief, true. _843_7001_10_01 42_
Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. AREA. NUMBER OFFICER OR AUTHORIZED AGENT NME YEAR MO DAY CME TYPED OR PRINTED (ernc rnrl)r"I
- COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattaclimentsniet' The Metal Ponds (Outfall 107) were discharged directly to the LVWTP (Outfall 103) on 12/3/09, 12/8/09, 12/10/09, 12/15/09, 12/16/09, 12/17/09, and 12/22/09. No phosphorous bearing cleaning solutions were used."
EPA Form 3320-1 (REV 3/99) Previouseditions may be used " Page I of 1
PERMITTEE NAME/ADDRESS [Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
, TVA- SEOUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRR Name (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000
- (INTEROFFICE SB-2A-SN)- TN0026450 110 G F -FINAL SODDY - DAISY. TN 37384 PERMIT NUJMBER DISCHARGE NUM3BER RECYCLED COOLING WATER Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY EFFLUENT Fro "MNIT("0RINi P:RIOD
- YEAR MO I DAY- 1 YýR ý MQtIDAY
- NO DISCHARGE ATTN: Stephanie A. Howard FromI 09 L 12 1 01. 1 To.. 09 OS 2 3 NOTE: Read instructions before corftoletinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EXi OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIs TEMPERATURE, WATER DEG. SAMPLE 04 ***"
CENTIGRADE MEASUREMENT 04 00010 Z 0 0 "'PERMJT i! DEG C , "38.3 DEGC.C DAILYG GRAB.24 INSTREAM MONITORING REUIEMNT PH SAMPLE ** 12 MEASUREMENT EFFLUENT GROSS VALUE MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE ** 19 MEASUREMENT 00530 1 0. 0 PIE RMIT, ****i* ' i 30 ' MG/L DAILY COMPOsjS EFFLUENT GROSS VALUE REURMND AILY MX OIL AND GREASE -. SAMPLE . .*1.
MEASUREMENT 19.
00556 1 0 0 PERMIT .***.. ,15 MG/L -~DAILY GRAB EFFLUENT GROSS VALUE ;.FOLIREMENT : i:y AL FLOW, IN CONDUIT OR THRU SAMPLE 03***
TREATMENT PLANT MEASUREMENT 50050. 1 0 0 REPORT .....MGDE*. i . DE-REPORT CALCTD EFFLUENT GROSS VALUE MO AVG DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 50060 1 0 0 PERMIT '.0.10 ' GLWEKLY GRAB-4 EFFLUENT GROSS VALUE REURMN 'DAILY MX SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE. PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my " TELEPHONE" DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church . properly gather and evaluate the information submitted. Based on my inquiry of the person or ". ;;
Persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01 07 Sequoyah I Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED OFFCOERDORAUTHORIZEDAGENTAREANUMBERYEARMO_ DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference ?//attachments/e76 No Discharge this Period "
EP For 3301(E 39) Peioseiinmyb sd ae1o EPA Form 3320-1 (REV 3/99) Previouseditions may be used Pagel1 of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEOUOYAH NUCLEAR PLANT OMB No. 2040-0004 (SUBR 01)
Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON) TN0026450 110 T F-FINAL .
.SO__DDY - DAISY. TN. 37384--
- PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Facility TV - SEQUOYAH NUCLEAR PLANT Locatio . HAMILTON COUNTY .......... F _ ~MnrtwTnCW1rur DCVIrfl 7 EFFLUENT ATTN: Stephanie A.Howard From] 09 122011 T 311 NO DSCHARGE NOTE: Read instructions before completinq this form.
PARAMETER
- EX- OF TYPE AVERAGE MAXIMUM, UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS
- 2 IC25 STATRE 7DAY CHR SAMPLE CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 -PERMIT : ** ,,4..2 :.. PERCENT SEMI Z -OmbS EFFLUENT GROSS VALUE IELRMN : ibi.MiNIMUM .' - .. , ANUA .
IC25 STATRE 7DAY CHR SAMPLE . .. 23 MEASUREMENT 23 PIMEPHALES" TRP6C 1 0 0 /. PERMIT. . ********,, 45.... PERCENT .SEMI. COM0OS EFFLUENT GROSS VALUE - - MEINUUREMEM
. .. -- ANNUAL iK.$i- .
SAMPLE MEASUREMENT
~PERMIT,,
REQUIREMENT.
SAMPLE MEASUREMENT PERMIT REAUIREMENT' SAMPLE MEASUREMENT YPERMIT:
~REQUIREMENT SAMPLE MEASUREMENT,
-PERMIT.
SAMPLE MEASUREMENT
<PERMIT-R5E,)UIREMENT.
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my. , TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified person_ __el_ _
Christopher R. Church properly'gather and evaluate the information submitted, Based on my inquiry of the person o, persons *vho manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WBN 8 1 information, the information submitted is, to the best of my knowledge and-belief, true, __423 "_843-7001,_ 1_0 01 07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED T AGENT AREA NUMBER YEAR MO DAY information, including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshe.
No Discharge this Period EPA ForrTi i320-
_ 1 (REV 3199) Previous editions may be used -:Pagel1of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (tDMR)
(SUBR 01) OMB No. 2040-0' 004 Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON) F - FINAL
-* ZTN(0Z45 116 G SODDY - DAISY. TN 37384. PERMIT NUMBER DISCHARE NUMBER BACKWASH Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY Fro NITtRING PERIOr)D EFFLUENT SYEAR } eI DAY L JYEAR J MO DA' From o9T12 1011 To[ 09T 12 311 NO DISCHARGE . **
ATTN: Stephanie A. Howard NOTE: Read instructions before comoletina this form, PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE
- 0** 0 1 / 31 VISUAL MEASUREMENT 0145 1 .. ~R EQUREMENT' .'~* ****"'~ ****"" REPORT PASS=O , K, SEE VISUAL EFFLUENT GROSS VALUE ,REIEMN 'i , , ', . MO TOTAL FAIL=1 9'.PERMIT..
OIL AND GREASE VISUAL SAMPLE 94 * " 0 1 /31 VISUAL MEASUREMENT 84066 1 0 0V PERMIT . , REPORT i****iM* ;. YES=Io1 i , * ' -. SEE VISUAL EFLET RS VLEREQUIREMENT > ' . ,NO4 PERMIT.
SAMUNTGRSLEAU MO TOTAL = .~ " ,.'PRI SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT
'PERM[IT' REQUIREMENT PERMIT' SAMPLE REQUIREMENT MEASUREMENT SAMPLE MEASUREMENT PERMIT .
REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER tICertify under penalty of law that this document and all attachments were prepared under mY. .i -)* I i
- TELEPHONE DATE
" DATE.,,,
direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church ' property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly, responsible for gathering the Environmental Manager.SQN & WBN 423 843-7001 10 01 07 h information, the information submitted is, to the best of my knowledge and belief, true, _ ___843400___0___ __07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
- " linformation, TYE RPITD-OFFICER including the possibility of fine and imprisonment for knowing violations. O OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTEDCOD COMMENTS ANDEXPLANATION OF ANY VIOLATIONS (Referenceallattachmentshel Operations performs Visual inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199) . Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM, (NPDES) Form Approved.
MAJOR TVA- SEOUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name :. (SUBR 01)
Address P.O. BOX 2000 "" TNO026450 "17 G 1i" 1 (INTEROFFICE SB-2A-SON) F- FINAL SODDY - DAISY. TN 37384 PERMIT NUMBER _DISCHARaGE NMBER BACKWASH FacilitY TVA - SEQUOYAH NUCLEAR PLANT LoCatio HAMILTON COUNTY From MONITOjRINGPRIO 1 EFFLUENT IY EA R M(' I DAY
- I YýAL M r) I nA FromI 09 112 1101 1TOl 09 lý112- 131{ Y I NO DISCHARGE,
- ATTN: Stephanie A. Howard NOTE: Read instructions before comoletinq this form.
PARAMETER , " OUANTITY
"__ _ OR LOADING 1 'EX QUALITY OR CONCENTRATION . NO. FREQUENCY OF SAMPLE TYPE*
AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE *. . ** * . 0 9A 0 1 /31 VISUAL MEASUREMENT 01345 1 0 0 ~PERMIT7. ~ ***~ **** ,Wo****.,, ,.< RPR PASS=0 K SEE, Vlt EFFLUENT GROSS VALUE REOUIREMEN ',.i.. ','. :1. , 'M< FAIL=I PERMIT OIL AND GREASE VISUAL EFFLU NT G OSS SAMPLE ALUEM 0 94**
OTOTAL 0 1 /31 VISUAL MEASUREMENT :. .
EFFLUENT84066 1 GROSSVALUE 0 PERMITEN . ,_ .. REPORT. Y.ES= . SEE VISUAL EFLETGOSVLEREQUIREMENT. MO TOTAL N = PEMI SAMPLE ...
MEASUREMENT j7PERMITh...............
REQUIREMENT SAMPLE MEASUREMENT PERMIT.
REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT
.~PERMIT.
REQUIREMENT SAMPLE MEASUREMENT PERMIT, REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my/I
- ,direction or supervision in accordance with a system designed to assure that qualified personnel f TELEPHONE DATE Christopher R. Church
.~-
. properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WBN
-I 42 8
' ,, S information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01_*_07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
- information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY 0 -______ ORATOIZDAET _R ARA_ YARM.DY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he" Operations performs visual inspections for floating debris and oil 'and grease during all backwashes.
EPA Form 3320-1 (REV 3/99)-ý Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR , : Form'Approved.
Name TVA - SEOUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address P.O: BOX 2000 (INTEROFFICE SB-2A-SON) EITNU2645ER 1 F -FINAL SODDY - DAISY, TN 37384 L PERMuITNUMBERJ LPJ511ARGE NUB WASTEWATER & STORM WATER Facilitv TVA - SEQUOYAH NUCLEAR PLANT-ftlý II EFFLUENT Locatio HAMILTON COUNTY I ~EI¶ILIJ ATTN: Stephanie A. Howard From YEAR 09 IMO 12 01AI YEAR MT DAY .
NNO DISCHARGE NOTE: eTot Read ti *before
.irnstructions b .. c1otnhfr comipletincl this form.
PARAMETER OUANTITY OR LOADING QUALITY OR CONCENTRATION . . NO. FREQUENCY SAMPLE EX OF TYPE
" AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE **
- MEASUREMENT 1 00300 1 0 0 .PERMIT 2.. . MG/L TWICE/ GRAS EFFLUENT GROSS VALUE , ' AI,-Y M, -
'-- ' WEEK 19 SOLIDS, TOTAL SUSPENDED SAMPLE ***
MEASUREMENT 19 00530 1 0 0 PERMIT 1 11 TWICE/
,....A......
EFFLUENT GROSS VALUE RE, Ri. , . ,
SOLIDS, SETTLEABLE SAMPLE * ** .25 MEASUREMENT 25 00545 1 0 0 . EMT....~*~* ***A* 9****' ,'1.0 MUL ONCE/ 'GRAB EFFLUENT GROSS VALUE' REIU IREMET M FLOW, IN CONDUIT OR THRU SAMPLE **********ONTH MEASUREMENT 03*
TREATMENT PLANT 50050 .1 0 0 PERMiT< REPORT ýREPORT MGD ***'* .~***. ***$.ONCE/ ESTIMA EFFLUENT GROSS VALUE REQUIREMENrtT MO AVG DAILY MX .ATCH i* . .... . ." 'REASUIREMENT SAMPLE MEASUREMENT F1ERMIT REQUIREMENT' SAMPLE tPERMIT MEASUREMENT
'REQUIR~EMENT' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my / -, - . " TELEPHONE DATE
-direction or supervision in accordance witi a system designed to assure that qualified personnel AT ' I 1 IVJL C. Ohristopher R. Church properly gather and evaluate the information submitted. Based onmy inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01 07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ICODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachmentshet During this reporting period, there has been' no flow from the Dredge Pond other than that resulting from rainfall.
EP.om32-RV399 rvoseiin a be sedPae. f EPA Form 3320-1 (REV 3/99) Previous editions may be used . . Page.1 of 1