Text

Discharge Monitoring Report for December 2009
	ML100150093
Person / Time
Site:	Sequoyah
Issue date:	01/11/2010
From:	Church C; Tennessee Valley Authority
To:	Cromer P; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML100150093 (117)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 January 11, 2010 State of Tennessee Department of Environment and Conservation

.Division of Water Pollution Control Enforcement & Compliance Section 61h Floor,. L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR DECEMBER 2009 Enclosed is the December 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have any questions or need additional information, please contact Ann Hurt at (423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.

Sincerely, Christopher R. Church Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Field Office Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue.

Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C,. 20555 y.l LA' Printed on recycted paper

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SNN)

SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAH NUCLEAR. PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR) i PERMIT NUMBER DISCHARGE NUM ER MFNIToRING PEEI"D YEAS I MQ IA m_

LYuEA I mO A

From 091I12101 1TO 09 1 12 1 31 MAJOR Form Approved.

(SUBR 01)

OMB No. 2040-0 F - FINAL DIFFUSER DISCHARGE EFFLUENT

- - NO. DISCHARGE IZ NOTE: Read instructions before completiric thi*s form.

004 PARAMETER OUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.

SAMPLE

- 13.9 0_o 31 /*31 MODELD CENTIGRADE MEASUREMENT 04 00010' Z

'0 0

PERMIT

.30.5 DEG.C.

SEE' CKREQ INSTREAM MONITORING REQUIREMENT PERMIT TEMPERATURE, WATER DEG.

SAMPLE

- 28.2 0

0 31/*31 RCORDR*

CENTIGRADE MEASUREMENT 00010 1

0 0

PER IT O.-

REPORT DEG.C.

.,.SEE

.CK RE'Q EFFLUENT GROSS VALUE

-REQUIR.EMENT.'

'i'7'"'-"

DAIL Y MX7 PERMIT..

TEMP. DIFF. BETWEEN SAMP. &

SAMPLE 29 0

0 31 /31 CALCTD UPSTRM DEG.C MEASUREMENT 0

00016 1

W 0

PERMIT 5.0 DEG. C...

CONTIN' CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAL MX PH SAMPLE 7.5 7.8 12 0

5/31 GRAB MEASUREMENT 00400 1

0 0

PERMIT SU WEEKLY 'GRAB EFFLUENT GROSS VALUE REQUIREMENT MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE 5

5 19 0

1./ 31 GRAB MEASUREMENT 00530 1

0 0

PERMIT.

3,'

','MOL30 100 11IL

. MONTHLY G

EFFLUENT GROSS VALUE IREME.Tij

.MO AVG.

DAILY MX' OIL AND GREASE SAMPLE

<5

<5 19 0

1 / 31 GRAB MEASUREMENT 00556 1

0 0

."PERMI,'K****

2.

5

'2 MGIL

<'MONTHLY GRAB EFFLUENT GROSS VALUE REQUIREMENT>

"Ki'."h"

K.'<,:

'AiDAIMX FLOW, IN CONDUIT OR THRU SAMPLE 1599 03 1 31 / 31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1

0 0

"PERMIT REPOR MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAIL

'M" R

T' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were.prepared under my

.TELEPHONE DATE

-irection or supervision in accordance with a system designed to,assure that qualified personnel.

Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WaBN Sequyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true,

_423 843-7001 10 01 07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED RER COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachmentshei No closed mode operation. The following inf6rmation is included in an attachment: 1. CCW Data 2. Veliger Monitoring Data EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 2

DMR Attachment CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons' Analysis Date/Time Analyst Method No water would come out of the pump. No sample could be obtained.

CCW CHANNEL.

Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/09/2009 @ 1010

<0.10 mg/I 12/11/2009 @ 0425 KMF EPH

NOTES:%

Mean#

of Water Mean# of Water SUB Sample Date

% Settlers Sample Date Asiatic LOCATION Gravid Asiatic COLLECTED BY ZMS3 Temp.D tC)

/

Temp. (*C),

LOCATION lamsm Clam.......

11/03/2009 11/10/2009 11/17/2009 11/24/2009 12/01/2009 12/08/2009 133 417 269 36 32 38 0

6.1 0

50 0.

0 16 16 16 15 13.5 11 11/03/2009 11/10/2009 11/17/2009 11/24/2009 12/01/2009 12/08/2009 76 25 0

18 0

0 16 16 16 15.

13.5 11 Inplant Inplant Inplant Inplant Inplant Inplant RCW RCW RCW RCW RCW RCW CMW CMW CMW CMW WE CMW I

I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-S-N)

SODDY - DAISY, TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT LoCatio HAMILTON COUNTY NATIONAL POLLUTANT. DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

PERMIT NUMBER

[_DSCHARG N

FomNITRING M

IyFAR? In MO AY I

ýY F AR Mn A

From{ 09 12 { 1IT{0 12 I3 MAJOR Form Approved.

(SUBR 01)

OMB No. 2040-01 F - FINAL DIFFUSER DISCHARGE EFFLUENT

".. NO DISCHARGE NOTE: Read instructions before completinq this form.

004 ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING

,QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL

. SAMPLE 0.015 0.025 19 0

12/31 GRAB MEASUREMENT

.1 0

1 1.41 GA 500.60 1

0 0

PERMIT.

0.10 0.10 MG/L I

WEEK-

.CALCTD EFFLUENT GROSS VALUE E

MI MO AVG INST MAX YS TEMPERATURE - C, RATE OF SAMPLE 0

62

- 3 31 / 31 CALCTD CHANGE MEASUREMENT 82234 1

0 0

PERMIT...

2' DEG C

iNTi CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX CIHR UUS SAMPLE MEASUREMENT PERMIT...'

REQUIREMENTP SAMPLE MEASUREMENT PERMIT.

REQUIREMENT,___

SAMPLE MEASUREMENT PERMIT..............

REQUIREMENT SAMPLE MEASUREMENT PERMIT; REQUIREMENtT SAMPLE MEASUREMENT I.PERMIT K

REQUIRýEMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my

(>,

TELEPHONE DATE

. direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church, properly gather and evaluate the information submitted. Based on my inqdiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN information, the information submitted is, to the best of my knowledge and belief, true,

_423 843-7001 10 01 07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA

NUMBER, YEAR MO DAY TYPED OR PRINTED

""o COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments bet EPA Form 3320-1 (REV 3/99)

Previous editions may be used.

Page 2 of,2 EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 2 of. 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 -

(INTEROFFICE SB-2A-SON)

SODDY - DAISY. TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

TN0026450 0

F-FINAL PERMIT uUMBER NUMBER DIFFUSER DISCHARGE MONITDDITPING, PE:RIDQ EFFLUENT o

a ToAY YER M

DANODICHARGEY From 09 1 10 01 1 To1 09 12 31 ODSHREL__

Form Approved.

CMB No. 2040-0004 NOTE: Read instructions. before completinq this form.

PARAMETER QUANTITY OR LOADING

-,OUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS BORON, TOTAL SAMPLE

.<0.20 0

1/92 MEASUREMENT.

- 19 01022 1

0 0

.<.PERMIT REPORT MGIL I

OTRLY" GRAB' EFFLUENT GROSS VALUE REQUIREMENT i

i SAMPLE MEASUREMENT PERMIT REQUIREMENT_,,

SAMPLE MEASUREMENT REASUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

'PERMIT

.ITEQU°REMENT'fl SAMPLE MEASUREMENT PERMIT REOUIREMENT SAMPLE MEASUREMENT

.REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER T Certify under penalty of law that this document and all attachments were prepared under my I

TELEPHONE DATE 1direction or supervision in accordance with a system designed to assure that qualified personnel c

Christopher R. Church property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN 423 843-7001 10 01 07 information, the information submitted is, to the best of my knowledge and belief, true, Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE i nformation, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA R

YEAR MO DAY TYPED OR PRINTED I

OFFICERORAUTHORIEDAGENTAREA NUMBER__EAR__O__

_Y COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceallattaclrmentshet Boron was sampled on 10/07/2009 @ 1237.

EPA Form 3320-1 (REV 3199)

Previous editions may be used Page I of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)

SODDY - DAISY. TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

OMB No. 2040-0004 (SUBR 01)

TN0026450 101 T F-FFINAL A

PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 I

nA'h I

r*~

l D

I*P EFFLUENT M

I" r-F.I[SWU FrYoAR m M DA' From 1 09 I12 Oil01TOY 0 a

1 3

NO DISCHARGE NOTE: Read instructions before cornpletinq this form.

PARAMETER OUANTITY OR LOADING OUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE Monitoring 23 CERIODAPHNIA MEASUREMENTNot Reqitre in Not Required TRP3B 1

0 0

PERMiT

.45.2 PERCENT SEE COMPOS EFFLUENT GROSS VALUE REQUIREMEN MINIMUM PERMIT IC25 STATRE 7DAY CHR SAMPLE Monitoring 23 PIMEPHALES MEASUREMENT Nontore ng 23

IE HA E Not Required

_OS TRP6C 1 '

0 0

PERMIT 45.2 PERCENT SEE G*OMPS EFFLUENT GROSS VALUE REQUIREMENT>

.A:,

4M5iM.IUMPER MIT SAMPLE MEASUREMENT PERMIT REQPUIREMENT

SAMPLE MEASUREMENT AERMIT REQUIREMENT.

SAMPLE MEASUREMENT PERMIT',

REQUIREMENTM SAMPLE MEASUREMENT PERMIT"

REQUIREMENT,.

SAMPLE MEASUREMENT PERMIT>

,REQUIREMENT~

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this.document and all attachments were prepared under my

[."

E OD direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church

. properly gather and evaluate the information submitted. Based on my inquiry of.the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WBN "euy hSt Vc rsd n

information, the information submitted is, to the best of my knowledge and belief, true, "423 843-7001 10 01 07 Sequoyah Site Vice accurate, and complete. I am aware that there are signifi'cant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the.possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED OFCRO AUHRIE AGNT ARAN UME ER M

A COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachlmentshe; Toxicity was not sampled in December 2009. Report from November 2009 toxicity sampling is attached.

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page I of I

December 10,_ 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING,.NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, NOVEMBER, 2009 Per your request, I am only submitting an electronic copy of the subject report.

The report provides results of compliance testing, using fathead minnows and daphnids. Outfall 101, samples collected November 15-20, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

Call me at (256) 386-2755 if you have any questions or comments following your review of the report.

Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):

Sherrard, R. M., PSC 1X-C Files, OE&R, CEB IB-M SQN November 2009M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: December 10, 2009

1. Facility / Discharger: Sequoyah Nuclear Plant TVA

2. County_ State: Hamilton / Tennessee

3. NPDES Permit #:. TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

,6. Receiving Stream-TennesseeRiver (TRM 483.6)

7. lQI0: 3,491

8. Outfall Tested: 101

9. Dates Sampled: November 15-20, 2009

10. Average Flow on Days Sampled (MGD): 870, 873, 1761

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

1.2. Test Dates: November 17-24, 2009

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead. Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72-.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2,72.6, 100 UV treated Intake: 100.0

16. Permit Limit Endpoint (%)" Outfall 101: IC 25 =45.2%

17. Test Results: Outfall 101: Pimephales promelas: IC25 > 100%

Ceriodaphnia dubia:. IC2.5 > 100%/

UV treated Outfall 101: Pimephales promelas: IC 25 > 100%

Page 1 of 102

18. Facility

Contact:

Ann Hurt Phone #: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364 21.TVA

Contact:

Cynthia L. Russell Phone #:(256) 386-2755

22. Notes: Outfall 101 samples collected November 15 - 20, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 1.00 percent. Exposure of minnows and daphnids to intake samples resulted

.in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah..

Page2 of 102

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2.

SampleType: Composite

-.- 3.

Sample Information,:

Date Date Date (MM-DD-YY)

(MM-DD-YY)

Arrival Initial (MM-DD-YY)

Sample Time (ET)

Time (ET)

Temp. t TRC*

Time (ET)

ID Collected Received

(°C)

(mg/L)

Last Used By 101 11-15-09 0944 to 11-17-09 1500 11-16-09 0844 11-17-09 1315 0.6,0.7

<0.10 11-18-09 1413 11-15-09 1014 to 111709 1315 1.1 11-17-09 1500 11-16-09 0914 11-18-09 1413 11-17-09 0745 to 11-19-09 1400 1011118091505 1.3;' 1.4

<0.10 1 11-18-09 0645 11180911-20-09, 1411 11-17-09 0805 to 11-19-09 1400 Intake 11-18-09 1505 0.7

<0.10 18-09 0705 11-20-09 1411 11-20-09 1405 101 11-19-09 0735 to 11-20-09 1440 0.8, 0.9

<0.1.0 11-21-09 1418 11-20-09 0635 11-22-09 1410 11-19-09 0756 to 11-20-09 1,405 Intake 11-20-09 1440 1.0

<0.10 11-21:09 1418 11-20-09 0656 11-22-09 1410

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 + 1.0°C) in a warm water bath.,

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 3,of102

Pimephales promelas

. Test Organisms:

Ceriodaphnia dubia In-house Cultures

1. Source:

Aquatox, Inc.

2. Age:

22.50-22.73 hours8.449074e-4 days 0.0203 hours 1.207011e-4 weeks 2.77765e-5 months old

<24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderatelv Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic.

4. Number of Replicates:

4

5. Organisms per Replicate:. 10

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101

.8. Test Temperature: Outfall 101:

10 1

11-17-09 1444 ET 11-17-09 1430 ET 11-24-09 1346 ET "11-24-09 1331 ET Mean = 24.8 0C (24.5 - 25.3 0C) 11-17-09 1500 ET 11-24-09 1402 ET Mean = 24.9°C (24.6 - 25.30C)

Test Temperature: UV-Treated Outfall 101:

Mean = 24.90C (24.5 - 25.30C) 9L Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in.one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for DH, and dissolved oxvgen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToXCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4of102

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 17 - 24, 2009 using effluent from Outfall 101.

Test.

Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100

.1.1.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 98 98 98 98 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100

100 98 90 88 88 Test Solutions Mean Dry Weight (mg) eSluent)s(re plicate number)

(% Effluent) 1 2

3 4

Mean Control 0.830 0.817 0.842 0.797 0.822 11.3%

0.857 0.820 0.819 0.839 0.834 22.6%

0.849 0.792 0.794 0.884 0.830 45.2%

0.782 0.832 0.849 0.772 0.809 72.6%

0.806 0.758 0.770 0.781 0.779 100.0%

0.852 0.792 0.697 0.716 0.764 Intake 0.397 0.778 0.801 0.889 0.716 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA, Lower Limit: NAA

TUa 100/LC 50: TUc = 100/IC2 5 Page 5 of 102

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a C-eriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 17 - 24, 2009 using effluent from Outfall 101.

Percent Surviving Test (time interval used.-: days)

Solutions 1

2 3

3

(% Effluent) 1

.2 Control 100 100 100 100 100 100 100 11.3%

100 100 100 100" 100 100 100 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 100 100 100 100

-72.6%

100 100 100 100 100 100 100 100.0%

100

.100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)

Data (re plicate number)

(%Effluent) 1]2 13 4

5 6

7 8

9 10 Mean

.Control 32-29 26 30 27 30 28 29 31 33 29.5 11.3%

32-31 28 31 30 30 30 28 29 33 30.2 22.6%

32 32 29 32 32 32 32 32 32 34 31.9 45.2%

33 34 33 31 32 36 30 34 33 32 32.8 72.6%

33 34 34 32 37 33 38 34 30 32 33.7 100.0%

35 36 32 36 34 36 38 36 38 38 35.9 IC25 Value:.> 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/IC 25 Page 6 of 102

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 17 - 24, 2009 using water from Intake Percent Surviving Test (time interval, used - days)

SolutionsrT (oluens 1

2 3

4.

5 6

7

(% Effluent),

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)

%Effluent).

Data (replicate number) ent 1

2 3

4 5

6 7

8 9

10 Mean Control 31 27 30 32 28 30 31 30 28 29.8 Intake 38 35 33 30 29 33 32 3133 32.5 IC25 Value: > 100%.

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa*= 100/LC5 o: TUc = 100/IC2 5 Page 7 of 102

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 17 - 24, 2009 using effluent from UV Treated Outfall 101.,

Test Percent Surviving "Solutions.

1 2

(time interval used - days)

(% Effluent)-

1 2

3 4

5 6

7 Control i00 100 100 100 100 100 100 11.3%

100 100 100 100

100 98 98 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 100 100 100 100 72.6%

100 100 100l 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(%Effluent) 2 (replicate number)

(%_Effluent)_

1 2

3 4

[

Mean Control 0.848 0.718 0.772 0.870 0.802 11.3%

0.876 0.857 0.764 0.755 0.813 22.6%

0.749.

0.796 0.854 0.806 0.801 45.2%

0.724 0.816 0.825 0.865 0.808 72.6%

0.890 0.775 0.856 0.814 0.834 100.0%

0.775 0.757 0.939 0.867

.-0.835 Intake 0.813 0.726 0.780 0.770 0.772 IC2 5 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA!

Lower Limit: NA

TUa = 100/LC 50: TUc = 100/IC2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25)

Pimephalespromelas November 17-24, 2009 1220 17-days KCI

0.79 g/L Ceriodaphnia dubia November 10-17, 2009 1115 6-days NaC1 1.07 g/L I - NaCI I

Page 8 of 102

PHYSICAL/CHEMICAL

SUMMARY

Water Chemnistry Mean Values and ]Ranges for Pimephalesprornelas and Ceriodaphnia dubia Tests, Non-treated Sequoah Nuclear Plant (SQN) Outfall 101 e a

.a

.a1fo Y......

performed November 17-24, 2009.

Test Sample ID Temperature (C)

Dissolved Oxygen (mg/L) pH (S.U.)

Conductance Alkalinity Hardness Total Residual Initial

. Final Initial Final Initial Final (fLmhos/cm) (mg/L CaCO3) (mg/L CaCO3) Chlorine (mg/L) 24.8 24.7 7.8 7.4 7.58 7.37 332 62 90 Control 24.7 -

24.8 24.6 -24.8 7.6 -

8.0 6.9

-7.7 7.42 7.79 7.20 7.56 325 336 61 62 88

.90 24.8 24.7 7.9 7.3 7.66

.7.32 308 1 1.3 %

f~ '2 1 7.....

i 247 250 24.5

- 249 7.7 8.2 6.9 7.6 7.57 7.79

'75.6 750 297 317 24.9 24.8 8.0 7.1 7.63 7.29 291 22.6%

...:7...............

24.7 -:25.0 24.6 25.0 7.8 8.2 6.3 7.6 7.53.7.74 7.11 -

7.50 280 299 24.9 24.8 8.0 7.1 7.61 7.29 256 45.2%

24.7 25.1 24.6 -

25.0 7.7 8.2 6.4 7.7 7.47 7.73 7.14 -

7.51 249 266 24.9 24.7 8.1 7.2 7.57 7.28 218 7 2.6 %

24.7 25.1 24.5 24.9 7.7. -

8.3 6.6 7.8 7.41 7.70 7.04 -

7.51 211 225 25.0 24.7 8.1 7.2 7752 7,25 172 75 87

<0.10

__100.0 24:7

- 25.3 24.5 - 25.1 7.7 8.3 6.5 - *7.7 7.32 7.67 7.01-7.46 166 -

181 62

-. 96 i69' 110

<0.10

<0.10 24.9 24.7 8.2 7.2 7.49 7.24 170 63

.75

<.0.10 Intake 24.8 25.0 24.6 -. 25.1 7.8 8..4 6.5 7.9 7.26 f. 7.64 '7.0I 7.49

.163 179 58 66 69 82

<0.10

< 0.10 24.8 25.0 7.8.

7.9 7.58 7.59 332 62 90 C o n tro l 24.6 -24.9 24.9 -253 7.6 8.0 7.7 8.1 7.42 -

7.79 7.51 -7.71 325 336 61 62 88 90' 1%

24.8 25.0 7.9 7.9 7.66 7.59 309 11"3%___

24.7 -

24.9 24.9 - 25.2

-7.7 8.2 7.6 8.1 7.57 -. 7.79 7.52 -.* 71 297 317.

"6%

24.9 25.0 8.0 -

7.9 7.63 7.59 291 "

" 2 24.7 -25.0 25.0 25.1 7.8 8.2 7.5 8w1 7.53 -7.74 7.52-7.71

-280 299 4.%

24:9 25.0.

8.0 7.9 7.61 7.58 256 4 5.2 %

24.7 25.0 24.9 - 258.2 7.6 7

8.2 7.47 -. 7.73 7.51 - 7.71 249 266.

24.9 24.9 8.1 7.9 7.57 7.57 218 7 2.6 %........-...

24.8-250 24.8-25.1 7.7 83 7.6 8.2 7.41

..7.70 7.48-7.69 211 225 25.0 25.0 8.1

7.9 7.52 7.55 172 75 87

< 0.10 24.8 -

25.2 24.8 -

25.2 7.7 8.3 7.6 8.2 7.32 _ 7.67 7.46 -

7.66 166 181 62 96 69 110

<o.1i0 a<01O 25.0 25.0 8.2 7.9 7.49 7.56 170 63 75

< 0.10 24.9 25.0 24.8 25.2 7.8 8.4 7.6 8.1 7.26 -7.64 7.46 -

7.66 163 79 58 66 69 82

<0.10 -

<0.10 i..............

i-.*......~.......

Overall temperature (C)

Pimephalespromelas Ceriodaphnia dubia

........i.i............

i....

i..........

..............i............

..i Average Minimum Maximum 24.8 24.5 25.3 24.9 24.6 25.3 Page 9 of 102

PRV5IUCAT /CIW.MIrAT STIMU ARV Water Chemnistry Mean Values and Ranges for Pimephales prom elas Tests, UJV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed November 17-24, 2009.

.......... i.....................

.............................. - * = : :

Test Sample ID Temperature (C)

Dissolved Oxygen (mg/L) pH (S.U.)

Conductance Alkalinity Hardness

....................... :1 Initial Final Initial Final Initial Final (tmhos/cm)

(mg/L CaCO3) (mg/L CaCO3)

Control 24.8 24.8 8.0 7.3 7.61 7.35 321 61 89 24.7 - 25.0 24.7 24.9 7.9 8.2 6.4 8.1 7.53 -

7.68 7.19 - 7.53 312 -

332 61 62 86 90 24.9 12 4.8 7.9 7.3 7.62 7.33 308 24.8 25.1 24.6 -

25.0 7.7 8.0 6.9 7.6 7.54 -

7.68 7.16

.-7.51 301 312 24.9 24.7 7.9 7.3 7.61 7.33 293 24.8 -

25.1 24.6 -

24.9 7.8 8.1 7.1 7.7 7.53 -

7.69 7.16 - 7.52 290 -

299......

25.0 24.8 8.0 7.3 7.59 7.31 265 45.2%

24.8 -

25.2 24.7 -

24.8 7.9 8.1 7.0 7.7 7.49 - 7.68 7.15 7.49 259 -

273 726%

25.0 24.8 8.0 7.2.-

7.55 7.30 218 24.8 -

25.2 24.6 -

25.0 7.8 -

8.1 6.8 7.7 7.433 -

7.65 7.11 7.48 214 2

223 100.0%

25.1 24.8 8.0 7.3 7.51 7.28 173 63 74 24.9.-. 25.3 24.7.-. 24.9 8.0 -

8.2 6.9 7.8 7.37 7.62 7.09 - 7.47 169 181 58--68 65

-~

78 25.0 24.7 8.1 7.3 7.51 7.26 171 63 72

_Intake 24.9 -

25.2 "24.5-24.8 8.0 8.3' 6.9 7.8 7.37.

7.6 7.07 7.47 160 89..

60 66 69 78.........

24.

252 2.5 4.88.0 8.3 6.9

7.

7.37 - 7.617.7

.4 16 18 60 6

69 i8 Overall temperatureC(C) p r...

......... :...:.........A

!S !.e.....

s.....e.a s Average 24.9

Minimum Maximum 24.5 25.3 Page 10 of 102

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected November 15-20, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were

>.100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this' study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples..

Page 11 of 102

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-82 1-R-02-013. (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia.

Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS /MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None 2". Ceriodaphnia dubia None Page 12 of 102

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance -logbooks.

3: Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM.2510 B.

7.

Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this-report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in glL KC1 or NaCl.

2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.

Sodium Chloride (NaC1 crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 102

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 2ls1 Edition, 2005.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 102

Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used, to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test.Sampling Page 15 of.102

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom Hypochlorlte mg/L mg/L TRC TRC 03/12/1998 0.016 03/13/1998 0,05:i 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0,013 03/17/1998 0.020 P

03/18/1998 i 0,018 09/08/1998 0.015 09/09/1998 0.003 09/10/1998 )

70.014 09/11/1998 0.0xo13l3 09/12/1998

.0 1 09/13/1998

<0 0001*

09/14/1998 0..008 02/22/1999

< 0.002 1 02/23/1999 0.005 02/24/1999 0009 02/25/1999 0.0

2.

02/26/1999 0,008 02/27/1999 0.001 1 02/28/1999

<0001 I 08/18/1999 0.015 08/19/i999 0.012 08/20/1999 0.023 08/21/1999 0.022 08/22/1999 i

0.022 08/23/1999.

0.025 08/24/1999 0.016 PCL-401 CL-363 Cuprostat-H-13OMT:

mg/L nig/L PF

~mg/L~

Copolymer DMAD mg/L Quat Azole 0.005 0,021 0.011

-- 0.021 0.019 0:015 0.015 0.015 A:

i 0.024 0.006 0.024 0.024 0.120 0.024 0.024 i!

0.024 00O06' 0.023 QO.020 Page 16 of 102

Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom PCL-2M

- PCL-401 CL-363 Cuprostat-

-1130M Hypochlorite mg/L mg/L mg/L mgi/l PF mg. L

mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000

<0.002 0.026 0.009 02/01/2000-0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0,006 02/03/2000 0.008

.0.027 0.009 02/04/2000 0.006 0i.027 0.009 0.005 0.109 020520<

0.002 oo 0.027 0.009 02/06/2000

< 0.002

0,027 0.009 07/26/2000

< 0.0057 0,055 0.019 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0d.-053 0.018 U04

. 0.108 07/29/2000

< 0.0088 0.(055 0.019 07/30/2000

<0.0076 0,055 0.019

-i 07/31/2000

< 0.0152 0

.055 0.019 0,006 08/01/2000

<0.0141 0:055 0.019 O0.005 12/11/2000 0.0143 0025 0020 0.005 12/12/2000 0.0092 0.025, 0.020 0.005 12/13/2000

<0.0120 0.025 0.020 12/14/2000

< 0.0087 0.25 0.020 12/15/2000.

0.0120 0,025, 0.020 0.005 12/16/2000

< 0.0036 0.025 0.020 12/17/2000,

<0.0036 0.025 0.020 08/26/2001 0.0017 0

06 0.021 0.006 08/27/200 1

<0.'0096 0.06.

0.021 0,005 0.0021 08/28/2001

<0.0085 0.06) 0.0021 08/29/2001

ý<0.0094 U159 0.020 0.005 0.0-11 08/30/2001

<0.0123 0,.06 0.021 0.005 08/31/20011

<0.005 1,0.059 0 0.020 1

I 11/25/2001

<0.0044 11/26/2001

<0.00119 0.024 0.02 0.005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001

<0.0089 0.022 0.019 0.006 1 1/29/2001........0.0132 0.024 0.02 0.007 11/30/2001

< 0.0043

'0 4

0.02 12/09/20011o

<0.0042 7.

12/10/2001

<0.0042-12/11/2001

<0.0104 12/12/2001 0.0128 0.024 0.02 0,008 12/13/2001

<0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 Page 17 of 102

Table-B:L.Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to -Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 - November 20, 2009 Date

& 6Sdifu*um Towerbrom PCL-:222.>,

PCL-401 CL-363 Cuprostat-H- 130M Hypochorite mg/L mig/L mg/L Img/L PF mg/IL mg/I TRC Phosphate Coopolymfier DMAD mgIL Quat TRC Azole' 01/102/2002

< 0.0079 0.023 0.02 0.006 01/03/2002 0.0042 0.023.:K 0.014 01/04/2002 0.0124 0024:

0.014 0.009 0 1/05/2002

< 0.0042--

01/06/2002

< 0.0042 01/07/20021

<0.0089 1 0.024 0

0.014 0.006 '

1 02/24/2002

< 0.004 02/25/2002

< 0.004 0.023 0,023 02/26/2002 0.0143 U23 0.03 0.023 0.007 02/27/2002

< 0.0041 0j!O.023 0.023 02/28/2002

< 0.0041 0,024

" 0.008 03/01/2002

< 0.0041 1 0.024 0.008 05/05/2002 C

~

K-'

05/06/2002

-0.058 0.02 0.014 05/07/2002 0

'*"C02058

-0.02 0.01r5, 05/08/2002 U

0.056 00.19 05/09/2002

-0.057 0U2 0.014 05/10/2002 0.056

-1 0.019 08/04/2002

<0.0058 08/05/2002

<0.0058 09,.053 0.018 0.025 08/06/2002 0.0092 0.053 0.018 08/07/2002.<0.0107

.0.055 0.019 0.007 08/08/2002

<0.0061 07&.055 0.019 0810912002 0.0152 0.054 0018 1 0.008 1 10/06/2002

<0.00497 1010712002.

0.0153 0.054-.

0.018 0.009 10/08/2002

<0.0092 0.018 0 00 10/09/2002 0.0124 0.053 0.018 0.007 10/10/2002 0.0134 j0.054 0.018 0.009 10/11/2002

<0.0042 1.

0.054 1 0.018 01/12/2003

<0.0035 rKiJ 01/13/2003

<0.006 9 0.025 0.019 0.009 0114/02003

<0.01318 0*.026 0.020 01/15/2003

<0.0063 0.026 0.020 0,009 0 1/16/2003

<0.0034 0.0126 0.020 01/17/2003

<0.0034 0.026 0.009 04/06/2003.

<0.0073 0.021 04/07/2003

<0.018073

~

.2 04/08/2003

<0.0 117 0.021-04/09/2003

<0 *0139

-0.02 1

0,016 04/10/2003

<0.0113 0.021 0.018S 04/11/2003

<0.0073 0.022 Page 18 of 102

Table.B-1.(continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date Sodium Towerbrom PCL-222 PCL-401 CL 363 Cuprostat-IHypochlorite mg/L S mgL*

mg/L mg/L

. PF ing/L TRC Phosphate Copolymer DMAD mg/L TRC Azole 06/15/2003<

<* 0.0045

'1 06/16/2003

< 0.0037 0.057 0.020 06/17/2003

< 0.0048 0.041 0.014 06/18/2003

< 0.0048 0,041 0.014 06/19/2003

<0.0085 0.058 0.020 06/20/20031

< 0.0048

<i0.058 0.020 08/03/2003

<0.0050 08/04/2003

<0.0050 0.058 0.020 08/05/2003

<0.0051 0,057 0.020 08/06/2003

<0.0084 0.0517 0.020-08/07/2003 0.0129 0.057 0.020 08/08/2003 0.0153 0.057*

0.020

>0,00 10/05/2003

4.

<00043 0 ý 01)057 0.020 10/06/2003

<0.0043

.0.057 0.020 10/07/2003

<0.0090 0.057

.0.020 10/08/2003

<0.0106 0.7 0,5.7 0.020 10/09/2003

, 0.0181 0.0)26 0.022 10/10/2003 0.0183 0.026 1

0.024 0.009 02/01/2004 0.0093 0.027<

0.009 02/02/2004

<0.0034

0. 02*6 0.009 02/03/2004

<0.0034 026) 0.009 02/04/2004 0.0124 0.062 0.009 0,009 02/05/2004

<0.0034 0.026 :

0.009 02/06/2004 0.0105 0.026 0.00 9 0.010 05/04/2-00-4

<0.0123 0.6-026.

0.019 05/05/2004.

<0.0144 0.026<

0.014 0.009 05/06/2004

<0.01 46 0.037 0.013 05/07/2004 0.0227 0.058 0.020 0,009 05/08/2004 0.016.

0.060 0.021 05/09/2004

<0.0104 0.058 0.020 07/04/2004 0.0217 0.057 0.01.9.

07/05/2004

<0.0085

, 0.057 0.020 i0,009 07/06/2004

<0.0077 0.058 0.020...

07/07/2004 0.0252

.0.056.

0.019 07/08/2004 0.0223 0.0 57 0.019 0009 07/09/2004 0.0182 0.057w 0.020 0,009

.V Page 19 of 102

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 L November 20, 2009 Date:

Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF. HI-130M Nalco Hypochiorit mg/L mg/iL mg/L mg/L mg/L mgi.

73551 e

TRC Phosphate Copolymer DMAD Azole Quat mg/L mg/i.L EG/PO 11/07/2004

<0.0187 "

0 0.014 11/08/2004

<0.0192 0.047 0.030 11/09/2004

<0.0233 0,048 0.016 0.0O41 11/10/2004

<0.0149 0047 0.016 0,041 11/11/2004

<0.0149 0.049 0017 0.043 11/12/2004

<0.0253 0.048 0.017 0.042 02/06/2005

<0.0042 0.028 0.010 02/07/2005

<0.0 116 0.028 0.010 0.007 02/08/2005

<0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005

<0,0042 0.028 0.0 10--

02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063

,~*

06/06/2005 0.0043 06/07/2005 0.0103 06/08/2005 0.0295 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.06 0.009 07/18/2005 0.0150 0.026 0009 07/19/2005 0.0163 0.026 0.009 07/20/2005 0.0209 0.026 0.009 0.014 07/21/2005 00242

-I0.026 0.009 07/22/2005 0.0238 0.0A 0.018 0.014 10/30/2005 0.0068 10/31/2005 0.0112 11/01/2005 0.0104 11/02/2005 0.0104 11/03/2005 0.0117 11/04/2005 0.0165 11/14/2005 0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 o.o0.0231 11/18/2005 0.0200 11/19/2005 0.0116 Page 20 of 102

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser. (Outfall.101) Discharge Concentrations of Chemicals. Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 20, 2009 Date jSodlum Towerbrom PCL-222K PCL-401 2Hypochlonite mg/L 7:mg/L I mg/L ing/L TRC Phosphate Copolymer TRC 11/12/2006

/

0.0055 11/13/2006 0.0068 11/14/2006 0.0143 11/15/2006 0.0068 11/16/2006 0.0267 11/17/2006 0.0222 11/26/2006

.0.0188 11/27/206 -

0.0138 11/28/2006 1

0.0120 20 11/29/2006 w.

0.0288 11/30/2006 0.0376 12/01/2006 0.0187 05/28/07 05/29/07 05/30/07 0.0084

-05/31/07 0.0103 06/01/07 0.0164 06/02/07 0.0305

.12/02/07 0.0241 12/03/07 K

0.0128 12/04/07 0.0238 12/05/07

'0.0158 12/06/07 0.0162 12/07/07 0.0175

.04/13/08

.,0.0039 04/14/08 0.0124 04/15/08 0.0229 04/16/08 0.0143 04/17/08 0.0120 04/18/08 0.0149 10/26/08 K0.0260 10/27/08 0.0151 10/28/08

.w 0.0172 10/29/08 0.0154 10/30/08 K

10/31/08 0.0086 Cuprostat-PF 14-13W Nalco 7H-150M MSW mg/L img/L 73551

. mg/L 101 Azole Quat mg/L

, Quat.

mg/L EO/PO-_

Phosphate 0,037 0.037 0

0.037-0.037-

-0.015

.06, 0.015

<7- 0.017 0,036 0.015

~-

0.036 0.015 0.017

~0.036 0.015

-0.015 0.011 4.000.018 004 0.30

- 0.041 0.030 0.041 0.030 Page 21 of 102

Table B-i: Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 -November 20, 2009

- Date.'...

02/08'/09 02/09/09

-02/10/09 02/11/09 02/12/09 02/13/69 05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09 11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09 T6o6'ebf6 POCL-222 PCL-401'_

CL3836)

Uposto~afPf H-130M ' Nalco-H-150M MSW mg/L mg/-IL mg/L ing/L mg/L.

mg/L<

73551 'mg/LJ 101 TRC.

Phosphate Copolymer.

DMAD Azole Quat mg/L Quat mg/L EQ/PG Phosphate 0.0197:-

0017 0.0237 0.017 0.0104

!**i

,:**(.': :!::*.*:.:;0.021 0.0155

~i!0.017 0.0q106

".0.017 0.0129 0.04150 0.0053 0.0396i'::i 0.0049, R0396*.**i:i:*

<0.0141 0.39 0.025

i**

!:i i

0.0152

0.0255

- i!iii

!i g!:.* i ;.*

0.0306:

0.0093

- *i:;;,i i;*~i**!:*i iii!:}

Page 22 of 102

Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 102

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of 1

e*ent: TVA Enviromental Testing Solution, Inc.

Delivered By (Circle One):

P;ect Name: Sequoyah NP Toxicity 351 Depot Street.

FedEx UPS.

Bus Client PV. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:

__________________________Adam Deimling NPDES Number: TN0026450 Phone:

828-350-9364 Stephen Williams:

Collected By: Adam Deimling & Stephen Williams Fax:

828-350-9368 Samples remained on ice throughout sampling and transport to lab. Dissolved Metals sample filtered and place on ice.

Field Identification/

Grab/Comp Collection Date/Time Container Flow' tit*

5S12l Sample Description Number &

(MGD)

Rain Event?

Colume (Mark as Appropriate)

C A

\\i..

S Date Time Yes If-Yes, o

Trace ET':Lo*,

Ar.iVa Temp..

Time,.,Appear-(m/d/y (S)Inches

~Number.

ac SQN-l10 1-TOIX A Comp 11/15/09 -

0944 -

1(2 5gal) 7b.3q 11/16/09 0844 o._._

SQN-, 0 l -TOX B Comp 11/15/09-0944-1 (2.5gal) 3 11/16/09 0844

/-D.

SQN-INT-TOX

Comp, 11/15/09 -

1014-1 (2.5 gal)

NA4.1 11/16/09 0914 Sample Custody - Fill In From Top Down

-* "'vitc,

m f" T ALt. &IAW14E Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time Adam Deimling 12-

/

30 ).'O r

/

Express Courier

//2-!& O Express Courierf LE-

________t-lO 3

S Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C, and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way'that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page_1_of 1

Cint: TVA' Environmental Testing Solution, Inc.

Delivered By (Circle One):.

P fject Name: Sequoyah NP Toxicity 351 Depot Street.

FedEx

'UPS Bus Client PV. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 GeneraleCmments:

_______________Adam Deimling NPDES Number: TN0026450

Phone:

828-350-9364 Jonathan Walker Collected By: Adam De-lig & Stephen Williams Fax:.

828-350-9368 Samples remained on ice throughout sampling and transport to Clab.

Dissolved Metals sample filtered and place on ice.

Field Identification /

Grab/Comp Collection Date/Time Container Flow Sample Description Number &.

(MGD)

Rain Event?

U>A Volume -

(Mark as Appropriate)

-ao try Se Date Time If Yes, No Trace

-ETS "o, Arrival Temp-By Time Appear (mm/dd/yy)

(EST)

Inches Number (1c)

SQN-101-TOXA Comp 11117/09-0745-1 (2.5gal) v 1

.7 11/18/09 0645'

_0

_3

1.

IC SQN-I01-TOXB Comp 11/17/09-0745=

1 (2.5gal)

R't.7"

%it 3*

.__11/18/09

- 0645.

0.-._.___

/is.

\\.

!S* ).,

SQN-INT-TOX Comp 11/17/09 -

0805-1 (2.5 gal)

NA 11/18/09 0705 0,16 A'4ý Sample Custody - Fill In From Top Down

'"0174 Relinquished By (Signature)'.

Date/Time Received By (Signature):

at-440-#

.%A-taie/Time

__________________________4

________1Ae.-r-Md Afs#,

ALL sAMPyte.i Adam Dei

((hng3j Express Courier 6/ý 2

]0r-1/id?

Express Courier4 *,

t9-lt'(A ISo e"

ETS It-c--

it. j Instructions: Clients should fill in all areas except those in the "Laboratory Use" block..Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for, Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY-RECORD Page l

of 1

nt: TVA Environmental Testing Solution, Inc.

Delivered By (Circle One):.

Piwect Name: Seqtioyah NP Toxicity 351 Depot Street.

FedEx UPS Bus Client Pt Number: N/A Asheville, NC Other(specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:

_Adam Deimling NPDES Number: TN0026450 Phone:

828-350-9364 Stephen Williams:_

Collected By: Adam 1eimling & Stephen Williams Fax:

828-350-9368 Samples remained on ice throughout sampling and transport to lab. Dissolved Metals sample.filtered and place on ice."

Field Identification /

Grab/Comp Collection Date/Time Container Flow Sample Description Number &

(MGD)

Rain Event?

Lýboiatlory Use Volume (Mark as Appropriate)

Collected l,! i:*-'iiii : ;! i-i i[l.i, *i; :% i7 i11 :*7;1.

I'iii;i 1141':

Date Time iYes If Yes, Trace ETS Log Arrival Temp.

By, Time Appear (mm/dd/yy)

(EST)

Inches

........ Numbe.

CC.

a..ce, SQN-10I-TOXA Comp 11/19/09-0735-1 (2.5gal) 11/20/09.

0635 1760.

SQN-101-TOX B Comp 11/19/09-0735-1 (2.5gal) I76*V6 0411260q

~4'4O

" '11/20/09 063520. 04:,*::*;!*:,.*;*:i:;#

,i i**

SQN-INT-TOX Comp 11/19/09-0756-1 (2.5 gal)

NA I.

11/20/09 0656 lII2Dp Sample Custody - Fill In From Top Down "4 Cu s..r Relinquished By (Signature):

Date/Time Received By (Signature):

D Tue z-¶ mwir

,,a *.ta.sh.A IL Adam Deunling c~6~

(~

-C r Vxpress Courier e6 Q 6'91n E Express Courier

/

t TS Q)

/~gq Instructions: Clients'should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way.that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or.UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

"ETS Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0).

Species: Pimephales.promelas Page 1 of 6

*A o

'I" Client: Tennessee Valley Authority FacilitY:. Sea uoyah, Nuclear Plant A

NPDES #:..

1OaO46"-

TOOZ6'I SO Project N;:

5' L.

CountY: R-ea4 Outfail: 101 Dilution preparation information:

Comments:

Dilution prep (%)

11.3 1

22.6 45.2 72.6 100J uent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (rmL) 2217.5 1935 1370 685 0

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:

Test information:

Organism age:

-1:5

" 0o.vs o

Randomizing template:

4peo.lul Date and times organisms

\\N-t11.O'

%aCO Incubator number and were born between:

shelf location:

Organism source:

6M% -'x q

X 1\\-O' Artemia CHM number:

JA*I'q%

Drying information for weight

___determination:

Transfer bowl. information:

pH = ".St S.U.

Date / Time in oven:

1".O*

Temperature =1A.'10 C Initial oven temperature:

6tvC Average transfer volume:

Date / Time out of oven:

03. LIPO S Final oven temperature:

1.0 "¢-

_______Total drying time:

Daily feeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analy st Outfall 101 Intake 0-

-4OO 0S oat t %

ll I-dk Pi 4

~ rio (H__

0

~(~j 0~

VA' Do~

aiIl1 101%OhvI.os' I t 5

nk nn A4 ncil-7n,- q, I AOW Iln - C w_

I-v.,--,

~~I~4~-I 7

4 it.

-0i

A 1:541 4A

. Control information:

Acceptance criteria Summary of test endpoints:

% Mortality:

07.

1

!20%

7-day LCso

! 007.

Average weight per initial larvae:

.I 1.I'L-NOEC OO.7 Average weight per surviving larvae:

-0. I L.

> 0.25tg/larvae LOEC

)1007.

ChV

>1007.

IC25 t oo7v SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 27 of 102

Page 2 of 6 F

S Environnwltal Testing Solutions, Inc.

Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101. Non-treated Date:

i

- i"

-Survival and Growth Data Day CONTROL 11.3%

22.6%

Non-treated A

B C

D E

F G

H I

J K

L 0

to.

1O 10 I0 10

iQ 0

1o i

0 10 to I_

to 10 iO 1t

/o /

i /0

/ 0 10

/0

/0 2

JQ1 10 1

(0 /to 10 t

0

O I0 to 10 I Io )

0 10 t0 I o

10

/0 to,.

/0" /b 6 /0 (0

10 ID

/0 10 10 10

/0 10 10 0

5 to ui 10 10 10 10 I

O /o0 1 t0 10 !0 6

).t1 I o

r o

1 o

1 I,

1O 1

11(0 to/0O 7_

10

./O t) 11 10 t

to 1 0

10 /0

/0 A = Pan weight (nmg)

T ra y co lo r co d e ::, Ih A L I.3;

(

".3 1

Analyst:

t~c 4

3D

(*

1.&

9i.

~

r

-1 qq~q~

Date: 10 -.Aj -2, B = Pan + Larvae weight (tu g )

2%

Analyst:, j 35 ZL-u zz.

?

z %.5 zz 2Z-3ýo Z34 1 Date: )z.,-jo C = Larvae weight (mg)

Weight per Initial number of larvae (mg)

= C / Initial number of larvae O'

N~

ci N%

ly o"

NN Average Percent

weight per reduction a.'

initial from number of control (%)

larvae (mg)

0.,

-1.*

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m - missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed: 4 -

IComments:.I Page 28 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I I

DTS EnvimnmmtAstin T

Solutnaons Inc.

Page 3 of 6 Species: Pimephales promelas"!

Client: TVA /Seauovah Nuclear Plant. Outfall 101. Non-treated Date:

!1-I- CA

________________Survival and Growth Data __________

.Day 42%

72.6%

100%

M" N

0r P

R S

T U

V W

X-I*o 1t I

1o tO t

It " 10 10 to 0

10 ID t.6 to

'0

/0 '/0

/0

/0 4(0

/O 0

/0 ftO

/0

/0 10 10 it t0 5

JO O

0 o10.Ii

/0 1

10

/0 to to lo I Fo 1.0o to Mo 0 o

/

/0 10 10 10 to L)10

10.

10 It) 10 10 to 10 0

1 A

Pan weight (rag)

Tray color code::

Analyst:

..l" I

"I1q.

II 16,S-1q,01. 1#.

JO1 i

1S

" )

I,36

,s,3 6

h, r I,,t.

Date:

!Q -

0----

B, Pan + Larvae weight (rag)

Analyst: *TJ 2,

7_q Zýb Oo z~l 2\\.sq z3.41q~

1Z.iz,

-L.1'Z 3

2.z,3D 0

-ý z

'0 t4.7 Date:

7.o\\-.

C = Larvae weight (rag)

-B-A in t R*".

.St "%.'0l "

' g6. -.

z.

2,,a1

(

-1.1 Weight per initial number I

V

--V

= C / Initial number of larvae Average Percent weight per reduction initial from control

0. A

t.

0.-(67

0..

-1 J. 7.

number of M%..

larvae (mg)

V Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded.

Calculations and data reviewed:

I I

Page 29 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09

0 FETSý,

') E~wfon,,,.taI Te tinSohtton nc.

Page 4 of 6 Species:-pimephalespromelas Client: TVAI/ Seauoyah Nuclear Plant. Outfall 101. Non-treated

.t

.L Date:

01q.j Day.

100% Intake Y

z AA BB 0

o t(0 ',

/~0 2

"z 2

/Q

'0 o -

10 10 10 10 3i*

i o

'0'j 4Lt0 1

6 t-7 A =-Pan weight (mg)

Analyst:

s Date: coo-c

-4) -0 9 B = Pan + Larvae weight Analyst:..

_.,)

Date:

%"ZNO',Vizl C - Larvae weight (mg)

=B-A m

.0 %

Weight per initial number of larvae (mag) 0.

= C / Initial number of larvae g.

Average

.Percent weight per reduction initial from control 0.1 1(

7.

number of

(%)

larvae m Comment codes: c = clear,.d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w wounded.

Calculations and data reviewed:

Comments:

i SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 30 of 102

Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24,2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Prject numnber:

5812

%,. I I÷ t E t C n a n a o atlua (% )

B np l lr o

[nla lt.as mb w F ~

sh e ho va.A -

~B P-s LN o t fo r C' c c I LOs C_ýtk. ~ ~

~

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~

h RP-f Inta..*-

zd.lohu A--

h1W L

ig) eig8.n3hift M

Wna

.~Iku ko~lat ofrarhlo.

Wdgo/i~llh.I.akaw Hue earotodt ofa Pt-t ed k

tIsa*

hWgkt(.-o

-"A-B mber or L-a (.)

Sa--v1vh$ mber, (M.

nfl..,.. (u.

(%)

ouallbrvrat',ae vo'h-n.

A.srd

(%)

A 10 10 13.60 21.90 8.30 0.830 0.830 B R 10 10 14.25 22.42 8.17 0.817 0.817 Control C

10 10 13.11 21.53 8.42 0.842 0822 2.3 0.842 1000 0S22 23 Notapplicahle D

.10 10 15.55 23.52 7.97 0.797 0.797 E

10 t0 13.18 21.75 8.57 0.857 0.857 11.3%'

F 10 10 14.47 22.67 8.20 0820 0-834 2.2 0.820 100.0 X014 12.2-

-. 5 G

10 10 14.12 22.31 8.19 0.819 0.819 H

10 10 13.60 21.99 8.39 0.839 0.839 1

10 10 13,09 21.58 8.49 0.849

.0.849 22.6%

10 10 14.93 22.85 7.92 0.792 0.830 3.4 0.792 10050 a4.3 s.,

-I.0 K

10 10 14.42 22.36 7.94 0.794 0.794 "A-L 10 10 14.65 23.49 8.84 0.884 0.884 M

10 10 13.13 20.95 7.82 0.782 0.782 N

10 9

14.12 22.44 8.32 0.924 0.832 8.5 0.832 9080 4.6 1.6 45-2%

0 10 10 13.99 22.48 8.49 0.849 0.849 P

10 10 11.91 19.63 7.72 0.772 0.772 10 10 14.05 22.11 8.06 0.806 0.806 72.6%

R 10 10 14.01 21.59 7.58 0.758 0.779 2.6 0.758 100.0.

5.2 S

10 10 15.77 23.47 7.70 0770' 0.770 0779 2.6 T

10 10 14.91 22.72 7.81 0.781 0.781 U

10 10 13.61 22.13 8.52 0.852 0.852 100%

V 10 10 14.38 22.30 7.92 0.792 0

0.7 W

10 10 13,83 20.80 6.97 0.697 0.697 1

X 10 10 15.26 22.42 7.16 0:716 0.716 Y

10

" S 14.87

. 18.4 3197 0.794 0397 1010 10 13.41 2.1,9 7.78 0.778 0.816 6.1 0.778 875 0.716' 30.5 12.8 AA

.10 104.49 22.50 8.01 0.801 0.801 B9l 0

44 23.29 8.89 0.889 0.889 Outilall 101:

Dennett's MSD value:

PMSD:

Intake:

Danuett's MSD value:

PMSD:

0.0683 8.3 02577 31.4 MSD =

Minimum Significant Difference PMSD =

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declara1 statistically significant in a whole effluent toxicity te.

Lower PMSD bound determined by USEPA (I0th percentle) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 300.W Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interluhoratory Variabiliy Study (USEPA. 2001 a;.USEPA, 2001b).

USEPA 2 001a, 2001b. Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods% Volumes I and 2-Appendix. EPA-821-B-0I-004 and EPA-821-B-01-005. US Environmental Protection Agency. Cincinnati, OH

t~J 0

SEnvironmental Testing Solutions, tnc.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:

11/17/2009 Test ID:

PpFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitorilg'Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

Non-treated Conc-%

1 2

3 4

D-Control 0.8300 0.8170 0.8420 0.7970 11.3 0.8570 0.8200 0.8190 0.8390

.22.6 0.8490 0.7920 0.7940 0.8840 45.2 0.7820 0.8320 0.8490 0.7720 72.6 0.8060 0.7580 0.7700

.0.7810 100 0.8520 0.7920 0.6970 0.7160 Intake 0.3970 0.7780 0.8010 0.8890 Transform: Untransformed I-Tailed Isotonic.

Conc-%

Mean N-Mean Meaný Mm Max CV%

N t-Stat Critical MSD Mean N-Mean D-Control 0.8215 1.0000 0.8215 0.7970.

0.8420 2.345 4

0.8283 1.0000 11.3 0.8338 1.0149 0.8338 0.8190 0.8570 2.162 4

-0.432 2.410 0.0683 0.8283 1.0000 22.6 0.8298 1.0100 0.8298 0.7920 0.8840 5.397 4

-0.291 2.410 0.0683 0.8283 1.0000 45.2 0.8088 0.9845 0.8088 0.7720 0.8490 4.641 4

0.450 2.410 0.0683 0.8088 0.9764 72.6 0.7788 0.9480 0.7788 0.7580 0.8060 2.626 4

1.509 2.410 0.0683 0.7788 0.9401 100 0.7643 0.9303 0.7643 0.6970 0.8520 9.351 4

2.020 2.410 0.0683 0.7643 0.9226 Intake 0.7163 0.8719 "

0.7163 0.3970 0.8890 30.456 4

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98188138 0.884 0.392664346 0.32317638 Bartlett's Test indicates equal variances (p = 0.14) 8.39288521 15.0862722 Hypothesis Test(l-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100

>100 1

0.06829131 0.08313002 0.00325397 0.00160593 0.123331696 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point SD 95% CL(Exp)

Skew IC05 65.141

'dIo IC15 IC20 IC25 IC40 IC50

>100

>100 sqnlOJJJ-17-O9data

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake:,.,

Non-treated November 17-24, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:.

11/17/2009 TestID:

PpFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821 -R-02-013 Test Species:

PP-Pimephales promelas Comments:

Non-treated Conc-%

1 2

3 4

D-Control 0.8300 0.8170 0.8420 0.7970 11.3 0.8570 0.8200 0.8190 0.8390 22.6 0.8490 0.7920

.0.7940 0.8840 45.2 0.7820 0.8320 0.8490 0.7720 72.6 0.8060 0.7580 0.7700 0:7810 100 0.8520 0.7920 0.6970 0.7160 Intake 0.3970 0.7780 0.8010 0.8890 Transform: Untransformed I-Tailed Conc-%

Mean N-Mean Mean -

Min Max CV%

N t-Stat Critical MSD D-Control 0.8215 1.0000 0.8215 0.7970 0.8420 2.345 4

11.3

.0.8338 1.0149 0.8338

'0.8190 0.8570 2.162 4

22.6 0.8298 1.0100 0.8298

.0.7920 0.8840 5.397 4

45.2 0.8088 0.9845 0.8088 0.7720.

0.8490 4.641 4

72.6 0.7788 0.9480, 0.7788 0.7580 0.8060 2.626 4

100 0.7643 0.9303 0.7643 0.6970 0.8520 9.351 4

Intake 0.7163 0.8719 0.7163 0.3970 0.8890 30.456 4

0.961 2.353 042577 1 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.82512182 0.749

-1.7159101 4.30798649 F-Test indicates unequal variances (p = 2.30E-03) 128.264832 47.4672279 Hypothesis Test (I-tail, 0.05)

MSDu MSDp MSB MSE F-Prob df Heteroscedastic t Test indicates no significant differences 0.25768323 0.31367405 0.02215513 0.02397863 0.37355912 I, 6 Treatments vs D-Control sqnlOJ_11-17-09data

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated November 17-24, 2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0

ýý FS

Daily Chemical Analyses Environmental Testing Solutions, Inc.

Project number 5812 Reviewed by:

'Concentration Parameter DDIy_5a pH (SUI) 7.481 7.50 7.631 7.41 7.501 7.44 7.711 7.56 7.55 7.0 7.421 7.231 7.79 7.24 DO (mg/L) 7.81 7.5 7.61 7.3 7.71 7.51 7.81

7.

7.9

.5 7.81 6.91 8.0 7.1 Conductivity jI~mboalcm)

.32 332ý Control Alkalinity (mg/L CaCO3)

Hardness (mg/L CaCO3) 90 Temperature CC) 24.8 24.6 24.8 24 24.8 24.7 24.8 24.7 24.7 24.6 24.8 24.8 pH_(SU) 779 7.46 7.66 7.37 7.5 7

7.731 7.50 768 71 757 720 762 715 DO 80 7.3 7.7 7.3 7.7 778 69 80 71 11.3%

Conductivit bo/cm) 317 3138032 Temperature CC) 250 24.6 24.9 24.9 24.9 24.7 24.58 24.7 248 24.8 24.8 pH_____(SU)____

7.74 7.44.

7.624 7.35 7.56 73 7.72 7.5 765 717 53 7.11 7.61 7.14 22.6%

DO (

_____g___

8.0 7.4 7.81 7.1 7.9

7.

~

8.2 76 8.0 73.9 638.1 6.8 22.6%

Conductivity (guhos/cm) 299 293 2682__8 rem____

250 24.8 24.9 24.9 24.8C 24.8 24.9 2482847 26448 P(S)7.73 7.44 7.61 732771 733 71 5

62 716

,47 715.59 71 "45.2%_DO L1 74 77 7

45.2%

Conductivity (guhos/cm) 266

__24__25 254 Temperature(C) 251 249 24.9 2

249 249 250 2498 2477 24.6 24.9 7H 7SU43 759 7248 733 767 75 760 7241 715 756 72% DO (mg/L) 8.1 7.3 7.7 70 7.9 7.1 821 78 8.3 74 8.2 6.6 8.2 6

72.6%_

Condutivit (I____m 25 2

27_1 217 211-Temperature CC)_________

25.1 24.8 24.9 24.9 249.25 25.01 24.81 24-8 24.7 247.26 24.91 24.51 Conductivity (junbos/cmr 100%

Alkalinity (mg/L CaCO3)

Hardness (mg.L CaCO 3)

Totai Residual Chlorine (mg/L)

<10 Temperature (C) 25.3 25.0 2

25.0 24.7 25.1 246 25.0 247 247 24.5 24.9 24.7 PH_______________

7.571 7.431 7.571 7.3 7.42 7.33 7.64 7.49 7.52 7.07 7.261 7.041 7.471 7.011 DO (

8.31 7.61 7.81 7.2 8:11 7.41 8.21 79 8.1 7.51 8.41 6.51 8.21 6.5 Conductivity (lunhos/cm) 100% Intake Alkalinity (mg/IL CaCO3)

Hardness (mg/L CaCO3)

Total Residual Chlorine (mg/LJ

<0.,

<0.0

<0.

Temperature C) 25.0 24.7 25.0

25.

24.8 24.7 250 246 25.0 24.6 2.9 24.6 24.9 24.7

.0 0

tnvironmental T l

utions, Inc.

Page 5 of 6 Species: Pimephales promelas..

Date:

Client: TVA / Sequoyah.Nuclear Plant, Outfall 101, Non-treated Dallv Chemistrv:

Analyst Concentration Parameter Conductivity (tImhos/cm)

CONTROL Non-treated Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L)

Temnerature (0C)

I 9

pH (S.U.)

DO (m2/L) 11.3%

Conductivity (Ltmhos/cmý TemDerature (00 I

I pH (S.U.)

I 1F,(o II q.?~3 I

DO (mafL) 22.6%

Conductivity (iimh s/cm)

I

,-s 0

-C II ':3.,

I Temperature (QC)

L5.O 4

DH (S.U.)

II '.*,t4LI I

1;.I II 7.132 I "1.S1 II

- 7 DO (mpL) 6.I 45.2%

Conductivity (A+/-M OS16m)

Temperature (M) pH (S.U.)

DO (mn/L) 72.6%

Conductivity (4MhosIm ASA I

-a Gi I

M -Cl IM. 0 1

Vk.11 1

_1J4 -,A I

lr4,,,

1 *14 8, PAO 1.4 A2 I "LS zt 11 j 3q 4, (.d,4 0

-7..

Temnerature (°0 pH (S.U.)

r DO (mg/L) 100%

Conductivity u

Alkalinity (mg CaCO31L)

Hardness

&2 (mg CaCQ 3/L)

TR chlorine (mgfL)

-Co. 1c0 Temnerature (0C')

Teprtr

.C).

oH (S.U.)

0 ~

DO (mg/L) 100%

Intake Conductivity (umOs/cm)

Alkalinity (mg CaCO 3/L)

____6_

Hardness (mg CaCO 3/L)

TR chlorinel(mg/L).

40.1I Temperature (QG)

U U

Initial U

SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 35 of 102

Sliecies: Pbnevh H I Clienti.TVA /'S Page 6 of 6 ailes gromelas eiuovah NuclEar Plant; Outfall 101. Non-treated Date:

I \\

Dav.. :"*'-

I I

Analyst

,L

. I...

4 vI~r ~-L& f~Y-5 6.

SII

! I:

.I :t

.. gL.ad-.

l

,Concen-

[Parameter tra-tion pH (S. U.-)

CONTROL Non-treated DO (mg/b)

Conductivity

-(gmhos/cm)

'3 )1-Alkalinity (mg CaCO39L)

Hardness (mg CaCO3/L)

I 1

1I I

1.9

.33-iA.1 3-4

.13 334.'I 1,

Tk-2 1

Temperature (M) i a

oH (S.U.I a-DO (m/UL) 11.3%

Conductivity (Ismhos/cm) 9117-

.0 50"T

ý03

-L-A --I OuA -

-Lk.4 1A.9 Temperature (MC) a a

oH (S.U.)

L 1 -7.50

-7.LOS 1 0 4-1 __3 25 3 11 11, DO (me/L) 22.6%

Conductivity (Jimhoslcm)

I I,.-rIL4'iA U

Temperature (M)

"Mq.i t

I 1A.

a a

a nHl (S.U.

cL-~i i -i~i I7&~-

I i-i~

I~-v II~

I1,

-q II'

,pH-- (S..../

DO (mg/L) 45.2%

Conductivity

[

(grmhos/crn)

-lus LU IýIlq 7.

-kao

-04_L 1

-71.1.11A1 MIA Temperature (°C)

Teprtr I-pH (S.U.)

1T-]'41 ;TI 1~ T II 404 F

In In Z

DO (mg/L) 72.6%

Conductivity 1 8.3

.1 q-Q 9.2

-11 U.(,__ F 7113 All 1.1tw 1" 'A M,

-Lk II A

I Temperature (°C)

"LS.0 11 p

I -

DH (S.U.)

/3 IA:1ý 11 -7. 4 1A 1 1.61:)

11. 4.001 1

F -~.'=.~--l*

DO (m/bL)

F.

100%

Conductivity (jimhos/cm)

Alkalinity (mg CaCO3IL)

Hardness (mg CaC0 3/L)

TR Chlorine (mg/L)

Temperature (00 9.0 T

Lou ko Ck I--------------

I I

1-I pH (S.U.)

I

~

]~c Iic2. I -. V-1I 1

14qq-9.01 1J

%04 AA7 I I.r,7-DO (mg/U 100%

Intake Conductivity (pmhos/cm)

Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L)

TR chlorine (mg/b)

Temperature ('C) 11t.o Fin.a..

I-tl I

Initial Final Initial Fi U

l I 1.1 II 4.,l1*(+

I l1 4M II tI I

I 3Initial Final I

Initial Final I

SOP AT20 - Exhibit AT20.3, revision 04-01-09

&MMEEMMM....WMAN Page 36 of 102

I I

S Page I of7 C'hronic, Whole Effluent Toxicit Test (EPA-821 R-02-6013 Method 1002.0)

Species Ceriodaphnia dubia

..Client:

Tennessee Valle6. Authorit Count:Hamiton Fa0cility: SeauovaluNitile Plant

-Outfall:

101'.:

-NPDES #:TNO026450 Project #: "-&VL.-

Dilution preparation information:

Comments:

Dilution prep (%)

11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0

Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information:

Test information:

Organism age:

< 24-hours old Randomizing template color:

MA.YME Date and times organisms were born en:

-T,0 V740 Incubator number and shelf between:

location:

2.6 Culture board:

A-,l-o A.

Replicate number 1 2 "3

4 5

6 7

8 9

10 Culture board cup number:-.

6 11 1,

Ot 101 IN I,

%M 'll I% YWT batch:

1*r*O*

Transfer vessel information:

I pH S.U.

Temperature =*,S.

'C Average transfer volume (mL): I C,,

PAe*

I n..bc Daily renewal information:

Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfail 101 Intake termination time 0

i1-'O ts)(

it-r.4 a*. -M0 k

C iI OS I,10.

0 llll-a SOi Aý0j.11 nlU.-0" Oa IM-0.O 3

IV-VAIitI 05 0-%%11cr 4.04 A

Ivl0i:&

__________f-lA 01W1.04 OR~~

M oa 4

6

.l...

iio f

i \\-

-0 A'J OS 1

IV &WJI

~5I" l,

7 1 it-14 A. 1 14 0 -L- -

Control information:

Summary of test endpoints:

Control-I Control-2

" Acceptance criteria

% of Male Adults:

.,20%

7-day LCso

> 1001.

% Adults having 3 ' Broods:

I

/o07, k 80%

NOEC

IOG1,

% Mortality:

520%

LOEC w7.

Mean Offspring/Female:

?'C.S "2-014 k 15.0 offspring/female ChV

),107,

%CV:

7.

5.qT.

. <40.0%

IC25 i.,

Page 7 of 102 SOP AT I1 - Exhibit AT! 1.2, revision 04-01-09

I.

S U

ETS Page 2 of 7

' Species:-C*eriodaohnia dubia.

Client-:. TVA / Seauovah Nuclear Plant, Outfa11101 Date:.

11"-

"0%

Survival and Revroduction Data, I.

I Replicate number 8

-T-Day 1

2 3

4 5

6 7

8 9

10 2

Young produced C)

C) 0 C) r)

0 0

0 Adult mortality U

L t

L_.

i.-

3 Young produced __

C C

0 0

)

Adult mortality t

U..

U.-

U 4

Young produced 6

Youngproduced IC 0

I 0 I t 0

Adult mortality

\\

U..

U

=

U.-

6 Young produced 0

C)

C L

c o

LiS b)..

0i.

Adult mortality

~

(~

U

~

\\

7 Young produced I$

L 1'

Total young produced 3

I 2-"

3 7.

Final Adult Mortality

C L.

s.-

U X for 3' Broods I "

.Z.-

Nowe Adult motoality (L live, D -dead). SB split brood (siogle brood split betwjeen two dayo),

CO =conry over (offspring cantied over with Adult durntg troansfer):

Concentration:

% Mortality:__________

n "

Mean Offsrin*e*male:

Sow: 11.3%

Survivalnd Reproduction Data S

.Replcatenumber R

Day 1

2 3

4 5

6 7

8 9

10 1

Young produced 10 I

c) 0 -

a-

=

"I Adult mortality L

L!.

U I

U..

L-L-

I Young produced 0 I

.l I o I

Adult mortality I

U I

U U

3 Youngproducedi 0

Il 0

I 0C)

Adult mortality U

I U.

I-4 Young produced IS

%A 4

1 q

S S

4 4

I Adult mortality l-

'L...

t-Il1 L-I tI Adult mortality U

L=

U U

U 6

Youngproduced i> "0 I"

l~

t IC)

In C

Adult mortality 6

Young produced I

.QI

_ I.

1 Total young produced 2Q t2.&

z.

Final Adult Mortality Mote.,

Adult mortality (L - live, D - dead),

SO split brood (oingle brood spli between two days),

CO - a-~ovea offsot carnied ov% tl dl otgtaar SConcentration:

% Mortality:

j Mean Offspring/Female:

.- 0

9/6 Reduction from Control-I:

..7-SOP ATI I - Exhibit ATI 1.2, revision 04-01-09 Page 38 of 102

[.

ETS Page 3 of 7 J

.Species: Ceriodaghnia dubia S

gClient:

TVA Seauoyah Nuclear Plant. Outfa ll 101..

Date:

I-k *-o I

Vurvival aind Ri'nrnduction Data o'. licate number Day 2

3 4

5-,

6

,"7

.8 9" J.

10 Young produced C

Q.

Iý

.)

(

'Adult motlty T.

z.

4 Young produced fl *__

Q..._ k"

-*kj Q

Adult mortality k._..

3 Young produced C

I" Adult mortality L_

I 4

Young produced Lk

\\_

S-.

Adult mortality

%L._

1._..

5 Young produced t'.

L I'

L I

[

l Adult mortality

\\._

t__

L a

s Total young produced B -..

Z

_1 2 t

,.*..7-

_%7 B 2

_ 5

.3 2-2--i a A*

minal Adult Mortaliy I-Z L..

Mote: Adult mortality (L live, D - de*ad), SB -split broo (single brood split between two days), CO can ame (offspringt carried over" with adult during transfer),

SConcentration:

% Mortality:

67.7U

_Mean Offspring/Female:

3

% Reduction from Control-1:

17.

CONe: 45.2%

Survival and Reproduction Data Replicate number.

Day

,1 2

3 4

5.

6 "7

18 9 "

10 Adult mortality 1

7 Young produced

0)

0.

L3q tA V3 I2$

I Adult mortalty "l_

5 k_

4o.

Young produced 0

31.

)

Adult mortality 1

4 Young produced lS

.L-l

~

LI Adult mortality

\\

6

.Yrung produced U

Adult mortality 7

Young produced t1' Total young produced 3 -

_ 44 2

3 0,3.

0(

al 0, Final Adult Mortality C

I L-C" L"

I Note: Adult mo rtality (L - Lim, - dead), SB - split brood (singe brood split between two days). CO - anw over (offip Ung carsed over yith adult during transfr).

I Concentration:

I

% Mortality:

0).

Mean Offspring/Female:

,3.L%

%Reduction from Control-1:

-t.

1:

SOP ATI 1 - Eihibit AT 11.2, revision 04-01-09 Page 39 of 102

TETS Page 4 of 7 Species: Ceriodaphnia dubla

..Cient: TVA / Sequoyah Nuclear Plant. Outfall 101 CONC: 72.6%

Survival and Date:

I.I-I1- 05 Reproduction Data I-I[

Replicate number Day 1

2 3-

`4 5

6 7

8 9

10

,.young prq!Fodued 0:C 0*

0':"(

(

i.*i.

C)

(*-

Adult mortalityý I

'2'-

-ded

-n 4

Youngproduced

..c 0

Adult mortality

.3 Young produced

\\

10.

(2)l.

2.

I*

\\

q Adult mortality Yu pocYoung producedAdult mortality_..

t_

S Autmortality t __ ~

5 Young produced VL If lt%%k VL Vb V6 11I 11 Adult mortality LL.~

6 Young produced 01 C) 0 00 Adult mortality

-IZ KJ C:)-\\..-'.

7 Young produced V0 t

I'-1 Totalyoungproduced 3o, 3?-

,i

3.

3

,4 3

3Z,-

Final Adult Mortality

'L_

C

\\

Note: Adult mortality (LC live, D - dead), SB - split brood (single brood split between two days), CO =

oarover loiffiping carried over with adult durinn transler).

Concentration:

% Mortality:

Mean Offspring/Female:

I

.'1

% Reduction from Control-:

1:

7-CONC: 100%

Survival and Reproduction Data Replicate number Day 1

2 3

4 5

6 7

8 9

10 1

Young produced p

Adult mortality L

!. C_-

IU,.

2 Young produced l 0j-Adult mortality L

L.

L

! j

'L-3 Young produced Adult mortality C.-C-j 5

Young produced to'L

'T Lam SAdult mortality t..

5 Young produced I

I~

iL I,

~-

Adult mortality

~

I C.

C 6

Young produced C)

C_. I.. rýQ 8C)

-C.

C)*

Adult mortality I- -

C-6 Young produced z (0 0

I p

Total young produced

,3

_N6 3i.

S 6.

314331.

3 9 a t.

a, a x f Final Adult Mortality N_.

\\Z I

C t 1110-ty %L 1-'.

080),.

-5pt roo.ks.%d.roousp1._ twoGyj, Concentration:

% Mortality:

7.

Mean Offspring/Female:

3.9

% Reduction from Control-I:

Page 40 of 102 SOP AT 11 - Exhibit ATI 1.2, revision 04-01-09

0Tr5-Page 5 of 7 il

,,-.'..Species-Cerlodaphnia dubla 2.

Client:. TVA SeqnuvihNuclear Plant,.Outfall 101 Date:

I %VO-0 CONTROL-2 Survival and Reproduction Data U

I Replicate number f_-

2 4 4 5'....6

.,7 8

9 10 Adult mortality U.-.

U U

U

'k U

U

'U-2 Young produced C)

Adult mortality IL ALI-..

]

Y Young produced

[

Adult mortality U

U U-U 4

Young produced

=

0A Adult mortality

\\.-

\\-

U-

\\I-4 Yong produced j-17 (0

Qj-Adult mortality C J

\\,-

C 6.[

Young produced 0

0 C)

C)

C-C)0..

Adult mortality

.UaU-\\

U 7

Young produced

-Lý

%=

Total young produced a

3c

k at)

~

.~2 Final Adult Mortality U-IX for 3rrs Broods

-?e,*-

No*e; Adult mortality (L = ive, D - deed). SB split brood (single brood split between two days). CO - catty over (offspring carried over with adult during transfer).

Concentration:

% Mortality:_

Mean Offspring/emale:

lo.01.*

CONC: 100% Intake Survival and Reproduction Data

[

Replicate number Dy1

.. 2 3

4,1 5

6-"

7-8 9

10 S

Young produced 0

0 Adult mortality U

0 U

U U

L.

U L

Young produced 1 9

1 Adult. mortality 9

L.

[..

3 Young produced 0-0 Adult mortality

\\.....

i._

U U.

4 Yo.ung produced Adult mortality L

U U

U.

5 Young produced 7

a I

Adult mortality U

T U

L L

C=

~

U U

U 6

Young produced C))

0 (a)Q Adult mortality

\\

U u

i L

U 7

Young produced 11 1l l-13 Il Is tS LS 11,0 Total young produced w{

-!sz" Final Adult Mortality U

N

,,*o.,z utm y k,* -uw, a&

"?

spr t k rise~ roxu SP t uwc~an two uays/,

ca rm Concentrdtion:

ove -~

~

J~

Itc mta qta

% Mortality:

Mean Offspring/Female:

i

%Reduction from Control-2:

-'. [7a 1 SOP ATI 1 - Exhibit ATI 1.2, revision 04-01-09 Page 41 of 102

0 TVA I Sequoyah Nuclear Plant, Outfall 101 - Non-treated November 17-24, 2009 Verification of Ceriodaphnia Reproduction Totals Control-I Day Replicate number Total 1

1 2 13 4

5 6

7 8

9 10 1

0 0

0

.0 0

0 0

2

0. 0 0

0 0

0.0 0

0 3

0 0

0

0. 0 0

0 0

4 4

4 5

5 4

4 6

6 46 5

13 10 10 12 10 12 12 12 13 11 115 6

0 0

0 7

15 15 12 14 12 13 12 13 12 16 134 Total 32 29 26 30 27

30 28 29 31 33 295 11.3%

Replicate number Total Day I

2 3

4 5

-6 7

8 9

10 1

0 0

1 0 0

0 0

0 2

0 0

0 3

0 0

0 4

5 4

4 4

4 5

5 4

4 4

43 5

11 13 12 13 13 12 12 10 12 12 120 6

0 0

0 7

16 14 12 14 13 13 13 14 13 17 139 Total 32 31 28 31 30 30 30 28 29 33 302 22.6 %

Replicate numberToa Day 1

2 3

4 5

1 6 17 8

9 10 1

0 0

0 2

0 0

0 3

0 0

0 4

4 5

4 4

6 4

4 4

6 5

46 5

14 11 12 12 12 12 1l' 13 13A 1 121 6

0 0

0 7

14 16 13 16 14 1617 15 13 18 152 Total 32 32 29 32 32 32 32 32 32 34 319 45.2%

Day Replicate number Total y 1 2 13 4

5 1 6 7

8 9

10 1

0 0

0 1 0 0

0 0

2 0

0 0

3 0

0 0

0

.0.

0 0

4 5

.5 6

4 5

"6 5

5 4

4 49 5

13 14 12 10 12 12 12 12 12 "13 122 6

0 0

0. 0 0

0 0

7 15 15 15 17 15 18 13 17 17 15 157 Total 33 34 33 31 32 36 30 34 33 32 328 72.6%

Re plicate number".

Day 1

2 3

4 5

6

' 71,r "7.8" 9

10 Tt 1

0 0

0.';'

0"o

!0

'0 0

2 0

0 0

3 0

0 0

0

' 0 0

01

... 0

.0

-0 4

5 5

4 6

6 4

6'

,5 '

5 74 50 5

12 12 14 12 13 13 A

13:"

14 II 11' 125 6

0 0

0.

0';

0 0

0 7

16 17 16 14 18 16 19:

15i: 14 17 162 Total 33 34 34 32 37 33 38-1 341 30 32 337 100%

Replicate number Total Day 1

2 3

4 5

6 7:

8-"

9 10 Total 1

0 0

0,.-. 0.

Q0..,0 0

2 0

0 0

3 0

0

0 0

0 0

0, "0

0 0

0 4

5 6

5 5

7 5

.6 6

56 5

14 12 10 13 13 12 15ý 11, 14 14 1 128 6

0 0

0 7

16 18 17 17 16 19

16.

20M 18 18 175 Total 35 36 32 36 34 36 381 '36

.38 38 359 Control-2 Replicate number Total Day 1

2 3

4 5

6 7

8

" 9 10 1

0 0

0 2

0 0

0" 0

.0 0

0 3

0 0

0 0'

0 0

0 4

4 4

6 4

5 4

4 4

4 43 5

13 9

12 12 11 13

12.

13ý

.12 10 117 6

0 0

0 F 0

.0 0

0 7

14 14 12 16 13 12 15 13 15 14 138 Total 31 27 30 32 28 30 31'.

30 31 28 298 100% Intake*

Replicate number Day 1

2 3

4 5

6 7

8 9

10 Total 1

0 0

00 0

0-0 0

0 2

0 0

0 3

0 0

.0 4

5 6

5 4

4 4

'.-5 4'

'.4.-,

5'

46.

5 14 12 11 12 12 13 12 12 12 12 122 6

0 0

0' 0 0

0 0

7 19 17 17 14 13 16 15 15 15 16 157 Total 38 35 33 30 29

,33 32 31,1 31 33 325

Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project nuim bet:

Rev lwedy:

K LtiýI 5812 Concentration Replicate number Survival Average reproduction I Coefient of Percent reduction from 0

.1 (offspring/f em le.. ]

O ip Cofi ln a

,Pre trd cinf

(% )

1.

2 3

.4 5

6 7

8 9

1 0

( % )

f.rn g.

e:va Wi at io n (% )

p o o led con trol, (% )

Control-1 32 29 26 30 27 30 28 29 31 33 100 29.5,

'7.4 Not applicable 11.3%

32 31 28 31 30 30 30 28 29 33 100 30.2 5.4

-2.4 22.6%

32 32 29 32 32 32 32 32 32 34 100 31.9'.

3.8

-. 1 45.2%

33 34 33 31 32 36

30.

34 33 32 100 32.8 5.1

-11.2 72.6%

33 34 34 32 37 33 38 34 30 32 100 33.7

. K 7.0

-14.2

.100%

35 36 32 36 34 36 38 36 38 38 100 35.9 5.3

-21.7.

Control-2 31 27 30 32 28 30 31 30

,31 28 100 29.8 5.4 -

Notapplicable 100% Intake 38 35 33 30 29 33 32 31 31 33 100 32.5 :.8.0..9.1 Outfall 101:

Dunnett's MSD value:

PMSD:

Intake:

Dunnett's MSD value:

PMSD:

MSD =

PMSD=

1.906 6.5 1.676 5.6 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower.PMSD bound determined by USEPA (10' percentile) = 13%..

Upper PMSD bound determined by USEPA (90' percentile) = 47%.

Lower and upper PMSD bounds were detcrmined from the 10th and 90th percentile, respectively, of PMSD data from EPA's'WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-AppendikxEPA-821-B-01-004 and EPA-821-B-01-005.

US Environmental Protection Agency, Cincinnati, OH.

.E

* " TS

~

Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Statistical Analyses

.t~j I

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:

11/17/2009 Test ID:

CdFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Outfall 10 1 End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubia, Comments:

Non-treated Conc-%

I 2

3 4

5 6

7 8

9 10 Control-I 32.000 29,000 26,000 30.000 27.000 30.000.

28.000 29.000 31.000 33.000 Control-2 31.000 27.000 30.000 32.000 28.000 30.000 31.000 30.000 31.000 28.000,-

11.3 32.000 31.000 28.000 31.000 30.000 30.000 30.000 28.000 29.000 33.000 22.6 32.000 32.000 29.000 32.000 32.000 32.000 32.000 32.000 32.000 34.000 45.2 33.000 34.000 33.000 31.000 32.000 36.000 30.000 34.000.

33.000 32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30.000 32.000 100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000.

Intake 38.000 35.000 33.000

.30.000 29.000 33.000 32.000 31.000 31.000 33.000 Transform: Untransformed Rank 1-Tailed Isotonic Cone-%

Mean N-Mean Mean Min Max CV%

N Sum Critical Mean N-Mean Control-I 29.500 0.9899 29.500 26.000 33.000 7:366 10 32.333 1.0000 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000 5.362 10 115.00 75.00 32.333 1.0000 22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10 137.00 75.00 32.333 1.0000 45.2 32;800 1.1007 32.800 30.000 36.000 5.142 10 144.00 75.00 32333 1.0000 72.6 33.100 1.1309 33.700 30.000.

.38.000 7.001 10 147.00 75.00 32.333 1.0000 100 35.900 1.2047 35.900 32.000, 38.000' 5.326 10 153.50 75.00 32.333 1.0000 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01).

1.045023799 1.035 0.04119118

.0.090545302 Bartlett's Test indicates equal variances (p = 0.46) 4.634984493 15.08627224 The control means are not signifieantly different (p =0.73) 0.350059429 2.100922029 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU Steels Many-One Rank Test t00

>100 1

Treatments vs Control-I Linear Interpolation (200 Resamples)

Point SD 95% CL Skew IC05

>100 ICIO ICI 5 IC20 IC25 IC40 IC50

>100

>100 sqn 101_11-17-09data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 17-24, 2009 Environmental Testing Solutions, Inc.

Statistical Analyses Used for PMSD calculation only.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:

11/17/2009 Test ID:

CdFRCR Sample ID:-

TVA / Sequoyah Nuclear Plant, Outfall 101 End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 TestSpecies:

CD-Ceriodaphnia dubia Comments:

Non-treated Conc-%

1 2

3 4

5 6

7 8

9 10..:.

Control-I 32.000 29.000 26.000 30.000 27.000 30.000 28.000 29.000

.31.000

.33. 000 Control-2 31.000 27.000 30.000 32.000 28.000 30.000 31.000 30.000 31.000 28.000 11.3 32.000 31.000 28.000 31.000 30.000 30.000.

30.000 28.000 29.000

. 33.000 22.6 32.000 32.000 29.000 32.000 32.000 32.000 32.000 32.000 32.000 34.000 45.2 33.000 34.000 33.000' 31.000 32.000 36.000 30.000 34.000 33.000

.32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30000 32.000 100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000 Intake 38.000 35.000 33.000 30.000 29.000 33.000 32.000

.31.000 31.000 33.000 Transform: Untransformed I -Tailed Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Control-I 29.500 0.9899 29.500 26.000 33.000 7.366 10 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000':

5.362

10.

-0.840 2.287 1.906.

22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10

-2.879 2.287 1.906 45.2 32.800 1.1007, 32.800 30.000 36.000 5.142 10

-3.959 2.287 1.906 72.6 33.700 1.1309 33.700 30.000 38.000 7.001 10

-5.039 2.287 1.906 100 35.900

.1.2047 35.900 32.000 38.000 5.326 10

-7.678 2.287 1.906 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <0 0.01) 1.045023799 1.035 0.04119118 0.090545302 Bartlett's Test indicates equal variances (p= 0.46) 4.634984493.

15.08627224 The control means are not significantly different (p = 0.73) 0.350059429 2.100922029 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100

>100 1

1.906063607 0.064612326 55.14666667 3.474074074 1.3E-09 5,54 Treatments vs Control-I sqnlO _1-1i-7-09data

io TVA I Sequoyah Nuclear Plant, Outfall.101 - Intake Non-treated November 17-24, 2009.

Environmental Testing Solutions, Inc.

Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:

11/17/2009 Test ID:

CdFRCR Sample ID; TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubia.

Comments:

Non-treated Conc-%

1 2

3 4

5 6

7 8

9 10 Control-I 32.000

-29.000 26.000 30.000 27.000 30.000 28.000 29.000 31.000 33.000 Control-2 31.000 27.000 30.000 32.000 28.000 30.000 31.000 30.000 31.000

,28.000 11.3 32.000 31.000 28.000 31.000 30.000 30.000 30.000 28.000 29.000 33.000 22.6 32.000 32.000 29.000 32.000

.32.000 32.000 32.000 32.000 32.000

'34.000 45.2 33.000 34.000 33.000 31.000 32.000 36.000 30.000 34.000 33.000 32.000 72.6 33.000 34.000 34.000 32.000 37.000 33.000 38.000 34.000 30.000 32.000 100 35.000 36.000 32.000 36.000 34.000 36.000 38.000 36.000 38.000 38.000 Intake 38.000 35.000 33.000 30.000 29.000 33.000 32.000 31.000 31.000 33.000 Transform: Untransformed Tailed" Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD.

Control-I 29;500 0.9899 29.500 26.000 33.000, 7.366 10 Control-2 29.800 1.0000 29.800 27.000 32.000 5.434 10 11.3 30.200 1.0134 30.200 28.000 33.000 5.362 10 22.6 31.900 1.0705 31.900 29.000 34.000 3.753 10 45.2 32.800 1.1007 32.800 30.000 36.000 5.142 10 72.6 33.700 1.1309 33.700 30.000 38.000 7.001 10 100 35.900 1.2047 35.900 32.000 38.000 5.326 10 Intake 32.500 1.0906 32.500 29.000 38.000 7.978 10

-2.793 1.734 1.676 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wiik's Test indicates normal distribution (p > 0.01) 0.951577842 0.868 0.62319944 1.12773217 F-Test indicates equal variances (p = 0.18) 2.563559294 6.541089535 The control means are not significantly different (p = 0.73) 0.350059429 2.100922029 Hypothesis Test (I-tail, 0.05)

Homoscedastic t Test indicates no significant differences Treatments vs Control-2 MSDu MSDp MSB MSE F-Prob df 1.676261504 0.056250386 36.45:

4.672222222 0.0120129 1, 18 sqn101_11-1 7-09data

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated November 17-27, 2009 1

Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test m tEPA-821-R-02-013, Method 1002.0 LKS Daily Chemical Analyses Environmental Testing Solutions, Inc.

-Project number:

5812 Reviewed by:

Concentration Parameter initial Final Initial I

Final Initial I

Final I

Initial Fnl Initial Fnl Initial I

Final I Initial Final PH (SU) 7.48 7.58 7.631 754 7.501 7.711 7.71 78 7.55 7.5

-*7.421 7.571 7.79 7.51 DO (mg/L) 7.8 7.8 7.61 7 7 7.71 8.01

.7.8.

8 79

8.

9 7.81 8.01 8.0 7.8 Conductivity (I~mhos/cm)3232M36341111111111

Control Alkalinity (mgIL CaCO 3)612=2 2

Hardness (mg/L CaCti))

______Temperature (C) 24.9 24.9 24.9 24.9 24.8 25.11 24.6 249.27

2.

24.71 24.91 24.81 25.3 pH SU) 7.79 7.57 7.66 7.56 7.57 7.711 7.73 768

.68 75 7.571 7.58 7.621 7.72 1.% DO (mWL) 8.0 77 7.7 76 7.7 8.1 8.2 81.81 8.01 7

1 8.0 7.8 1.%-Conductivityý(pnhos/cm) 317__

_T___

7_1330_37_0329

______Temperature CC) 24.9 24ý9 24.9 25.0 24.9 25.1 24.9 29 24.7

-50 24.7 25.2 24ý9 25.1 pH(SU) 7.74 7.57 7.62 7.55 7.56 7.71 7.72 78 7.65 7.4 7.53 7 59 7.61 7.52 DO (mgIL) 8.0 7.7 7.8 7.51 7.9 8.1 8.2

8.

8.0 8.0 7.9 81 8.1 7.8 22.6%

!Conductivity (pMmbos/cm) 2992326 2826

.286 280

______Temperature (1C) 25.0 25.0 24.9 25.0 24.9 25.1 24.9 20 24.7 250 24.7 25.0 24.9 25.0_

OH I!U1J!

1./Al

~f.~6I

~I.6II 7541 7.511 7711 7711 7661 7671 75tI 1471

~7tRI iWI

7~I 45.2%

7.73/L 7.5 7 M8.~

75__51_71 77_6_77 75 79 5

DO i

81 781 7.91

.111 81 7.9 8.01 8.0 8.1 78 Conductivity (Limbos/cm)

______5_29

ý 5425

ý Temperature f°C)

.24.71 24.91 24.71 25.01 2491 25 2 6-

6. -

DH (SU) 6.-

DO (I _/L) 72.6%

Cnand~r tlviii /..hkn/rmI r

Temperature (0C) pjH (SU)

[DO (mg/L)

Conductivity (Mahos/em) 100%

Alkalinity (mg/L CaCO3)

[Hardness (mg/L CaCO3)

Total Residual Chlorine (mt/L)

Temperature (*C) 7.70 7.56 7.591.

7.54 7.481 76 7.671 7.651 7.

8.1

.8 7.71' 7.6 7.9 80 8.21 8.21 225 221 22 2171 2

25.0

.24.8 24.91 24.9 25.0.

48

.25.01 25.11 2'

7.67 7.56 7.571 7.54 7.44 7.6 7631 7.62 7.

8.1 7.8 7.7 7.61

8.

8.0 8.0 8.2 181 1817 6

82 6

24.91 24.8 24.9 25.011 25.01.

25.0 25.0 24.8 2'

4.81 24.91 24.81 25.11

24.91 24.91 601

-2r_

741 7

.5

~ 6

.7,5674 8.31 7.91 8.21 8.01 8.2 7.

23ý_

1.

21 67 168 166 19J 2481 2481 2511 2491 25 oH iSUl 521 7.49[

7.26 7.56 7.47 7

100% Intake DO (mp/L)

Conductivity (Limbos/cm)

Alkalinity (mg/L taCti3)

Hardness (mg/L CaCti,) I___

Total Residual Chlorine (mg/L)

Temperature ('C) "

II

- a 1onmNr~* omo*i Page 6 of 7 I

Species: Ceriodadhnia dubla-Client: TVA / Sequogah Nuclear Plant, Outfall 101 Daly hemstry:

Date:

1 -,,

i Analvs t 1 Al AZI Day I

.1~

U Concentration Parameter I

I

,pH (S.U.)...

Conductivity (umhos/cm)

CONTROL Alkalinity (mg CaCO3/L)

Hardness (Mg CaCO 3/L) 41

-4 Temoerature (°CI I

I PH (S.U.)

11.3%

Conductivity, (iimhos/crn)

Temperature (°C) 14A I

22.6%

Conductivity (jimhos/cm)

Temperature (°C)

ILC. 0 1.S pH(S.U.)

1,

45.2%

Conductivity Temhos/cm)

A

,0 (0 1 Temperature ('C)

-S.O

,6 I -, -

II 7%1 I ".S*, 1I ' +1. I T~F1-~AF~TF I

I

,*J 1S V

¥,

IRA' L

iW I '.

I v4X LL5~

S pH (S.U.)

'4, -h 0 II 7.Sq I t.4PR 4 -'-~----4I----4.

-,,,1.

ai--

DO (mW,/L) 72.6%

Conductivity-I

,.ýmhs/ctn

'LS 02 Vk Temperature U

U oH (S9.11 j

1

ý1 DO (minL/

c~I 100%

Conductivity Ie Alkalinity

-LM-9CaC0 3/L)

Hardness (Mng CaCo 3 /L)

T!RKchlorine (mg/L) 01 1l0

.S.0 I

Temperature (°C) is. 0 U

S ~

l*l J

I nH (S.U.)

1.L42, a -~--n-DO (mLY/L I

100%

Intake Conductivity Alkalinity (Mg CaCO3/L)

(A Hardness (mg CaCh 3/L) (ra./L Til chlorine (mgfL 0

w

-72.

n r Ia.

Fin 0lal i~nitial Fi~nal Temperature (QC)

"1.L. 9 a

a Initial Page 48 of 102 SOP ATI I - Exhibit ATI 1.2, revision 04-01-09

r

- a 3

ss.um.A.tSJTbA*q 5*..,%

Page 7 of 7 Species: Ceriodaphnia dubia Client: TVA / Sequoiah Nuclear Plant, Outfall 101 Date:

(I-1-00i Concen-tration, -

'I Dav I

Parameter i

-P SU)-,

DO (mnfL)"

Conductivity (gmhos/cm)

_4 J--I Iv CONTROL Alkalinity (mg CaCO3,L)

Hardness (mg CaCO3/L) 1_1 -

ItAl

_LS.0

-Lk. I Temoerature (°C0 Temperature ('Q nh- (*

,~1 *~

1 cvA 1 '-

Ii.aI 4

1 -3Ii 1-HIt

'T 1"4atZ H q 11.3%

DO (mg/L)

Conductivity (jgmhos/cm)

'49 3 Temperature (0C)

II I-q p.

DH (S.U.)

22.6%

DO (mg1L)

Conductivity j (gmhos/cm)

Temperature (QC)

PH (S.U.)

DO (mg/L) 45.2%

Conductivity (Tamhos/cm) jTemperature (0CQ I f3, I I ý eýo 11 14 41 301 o.3

.1 11 2-1 ADS 3 1

04

a.

9 A 0-1 2-9 (P B (0 215 VAgk 1

0

-Ls. 0 1

1 -LS.0 ot

-L%. C)

TU2 5

2,14-ý B.-

6A 1910 IS-0 vk-1A.1

-LAA I

-Ls.-L-1j_(10q I -too

,'4 -sip e.z.

2_j "L5. I i %Q42-1.55 q.ALaa

'4, q'

'104ta nHl (S.U.t PH (S.a DO (mi_/L)

Conductivity (jumhos/crn Tempoerature (°C0 I

I

-emperature PH (S.U.)

DO (mg/L) p Conductivity 100%

('mhos/cm)

Alkalinity Hardness e(mrg CaCOtrL)

TR Chlorine (fng/L)

Temperature (°C)

I (07-61

< 0,10 I

1qA il MIA.

-LA-f I

_LS-1 p

DH (S.U.)

I

~ Iit~n3 I.2

-4, :ý1 I

(po I 1A[

100%

Intake DO (mgfL)

Conductivity

-7 Alkalinity m

CaC03/1, (gmhos/cm) 1(e "I Hardness (mg CaCO3/L)

TR chlorine (mgfL)

(0.10 Temperature ('C)

Z5.0 tg. o

-4. 0 7.,j 1

17Z.q Ls.

Initial Final I

Initial Final Initial Final F: Initial Fin Page 49 of 102 P

SOP AT I1 - Exhibit AT 11.2, revision 04-01-09

I:

I I

I S

0 9

ETSTl Page I of 6 6

. Chronic -Whole Effluent Toxicity Test (EPA-821-R-02-013 Metiod 1000.0)

... Species: Pimephalespromelas Client: TennesseeNalley uthority County:

d 1AMHILro'..J Facility: SequoyaNe Plat Oufall:101 NP DES#:

X_._.....L.,,

,,O,.

Projec#

5&Vý Dilution preparation information:

Comments:

Dilution prep (%)

1.3 22.6

'45.2 72.6 100 Each concentration was UV-treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes to remove pathogenic FDiluent Volume (mE) 2217.5 1935 "1370 685 0 -1Interferences.

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:

Test information:

Organism age:

1-1.So -0A

)Vt..

Randomizing template:

Date and times organisms Incubator number and were born between:

shelf location:

Organism source:

t Artemia CHM number:

j(AR4%%

Drying information for weight determination:

Transfer bowl information:

pH = *.6O S.U.

Date / Time in oven:

-0 Temperature =q.b1°C Initial oven temperature:

l C..

'Average transfer volume: "

Date / Time out of oven:

1!-*

Final oven temrerature:

(o 0.

__._,,,__Total drying time:

-,oj.

1360 Daily feeding and renewal. information:

I Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake

0 60 A____

30o 144001 it L -.

~

O~1O'S o

f~.O R-o 1o 1,62 01 It Vj o

-DS 0111ufLo(

11-17.-M 1-OV A0 O

b 1 I'mA A 1, o P a 4 t

i

,S I&

I %oaw

. 0 I

-3 '

11 "?-A-Oac No t, l qbt IZ.

m s ocit*,"

Ia I I.

a

.'Ot

- 1l It. 11*-f 4

o2-6oci

-~

IA Ims%-i.

N 7

-it-zq. 41 L3's Control information:

Acceptance criteria Summary of test endpoints:

% MortalitTy.

I

<20%

7-day LCo

)_1007..

Average weight per initial larvae:

862.

NOEC 10.,

Average weight per surviving larvae:

0.901-

"0.25mg/larvae LOEC iO,> 7.

ChV

> 1001.

IC25

[

10047.)

Page 50 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09

Suinsi Page 2 of 6 Species: Pimephales r6ielas..

Client: `TVA 1TSeaUovyah-Nuclear Plant. Outfall 101Uv-itreated

-~

Survivald and.Growth Date:a I-I"1*0' Data j.*.:.,*

.!?

9

f ;-

Daya

-CONTROL' :1.

3%

-. 22.6%

- V-treated A

B C

D E

F G

H I

J K

L to o0 to /0 1

tO

)

0

/0 /(00

/0

/0 2

j to I

/o I1 to /a0 /0 Gl0.tO to 0

20

/

tOI 10 0

1 10 1o 10 t

o to

/4 t

0 IO 10 10 10

/0 1/0 /0

/0 60 10 If I(0

/,)

/0 1(0 /0 0 /

.0 10

/6 1

I6 1

1I010 1

94 I0 i0 j Io.O

/0 -io

6.

/0~

10 1 O

go 1c101c 10 /0

.O

/0 to A = Pan weight (mg)

Tray color code::

Analyst:

Y*-*

1' I

1., -*

t I't.7 1'0 4":T-1'5 IS,7o H9.qI J-5.-t3 1i'/*5" 1146).1*

b Date:

10-?-," -tq-i B = Pan + Larvae weight (rag).

Analyst: fýkS 2Z.,',O aIS L..- o 22.7 t...Z.*J2 Zt.

?..Z 2,A-3 Zl.

-Z-Z.VI 2L.

0 2 2%A.4 Date:

w.ight (Om)

C = Larvae weight (mg)

=B-A gq "11.1I

,*$1

.)

)*

i*)8S

,(

Weight per initial number of larvae (mg)

= C / Initial number of larvae 0

P.,

'I 9.4 16 as V'

%0 Cs Average Percent weight per

reduction initial from 1.81.

number of j control (%)

larvae (mg) o~~g~s

(.got

.7 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, lg = unusually.large, d&r = decanted and returned, w = wounded.

sm = unusually small, Calculations and data reviewed:

IComments.*

Page 51 of 102 SOP AT20 - Exhibit AT2O.3, revision 04-01-09

I, D)ETS-SEnvironmnwrtal Testing SouinInc Page 3 of 6 I

r II Species: Pimephalespromelas.

Client: TVA / Sequoyah Nuclear Plant. Outfall 101. UV-treated Da

.Suvival and Growth ata te:

I%- i I-ai s

I I

Day"*.45&.2%

-*72.6%

100%

M N,

0 p~O

-R T

U V

W X

I (0

o iO 1

/0

/o to I0

/0o

/o 0o

/0 1

(0 tO 10

/0 to

/0 1

/0

/0 0

0

/

2 10 to

/Q to

.16 to t, t10

/0 10

/0 to 3

1IU.

/0 I10 /0 ID 0 /C

/0

/0 11D 0 I 4_ _./D 1

/

10 to (0

//0 1

I 0

1

.16 t0o 0

5

/0 10 o

0 10 1o I

i i

10

/0 10 10 (0 6

10 o t0

/b 1

0 / 6 O 0.(

0

/0 7

%-U_

/10 1l M0 10 10, 10

/0 10

/0 10 1tD A = Pan weight (mg)

Tray color code::

Analyst:. Y-I38.,;-7 14.9 1;? 1 L335' 1.547 j'T7

,-5

'ot7 q

l-.

/q.",i

"t Date:

1D - *'i -Q*1 B = Pan + Larvae weight (mg)

Analyst:

7 \\.43 2

l'l Z.Oi Z4-.S V7.72-Z3,('4

\\.Zt 7

O.C3 zz.12 2,*C Date:

" *\\:0 C = Larvae weight (mg) oB-A (

811

&%tS

&J0S j.i S.S 1.16 ltS 0

Weight per initial number of larvae (nig) oflra'4 5~~

=C / Initial number of larvae

9*

Average Percent weight per reduction 0.

initial from control 07' 0' g*5'

-q.{i.

number of (M-larvae.

)raI 13 II Ii I]

I I

Comment codes: c = clear, d = dead, fg = fungus, k= killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:4\\

Comments:

Page 52 of 102 SOP AT20 - Exhibit AT20.3, revision 04-01-09

I 1

0 S

0 9

DETS Envfr)Iuonmr nct.l ht Page 4 of 6 Species:.Pimebhales promelas Cl.,.,Client: -TVA-/ISeauoyah Nuclear Plant, Outfall. 101E. Vtreated

-- Surialand Gr6wth.-b

'Date:

[I-11-0*L.

Day 100% Intake Y

Z AA BB 0

tO

/0 2

10'0 10 o

.3 b~ '0 to M

4 i

5 1

0 10 D

5 1D 10

/0

/0 7

A = Pan weight (mg)

Tray color code:: /

Analyst:

13.. IS, IS-773 1 Date:

jo-1-0-g B = Pan + Larvae weight (mg)

Analyst: __.,A.5 2-3,53 ZOAIO Date: '

'1-, -'- I 0c C = Larvae weight (nag)

Weight per initial number of larvae (mg)

C

= C / Initial number of larvae N

Average Percent weight per reduction Initial from control number of

(%)

larvae (rg)I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed: -

I Comments:

SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 53 of 102

Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 17-24, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity.Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses

-n il, J."

Project nmber; 5812

+

Not for Comptliance Assesatoerst, MLaboranto7y eQCvei" ed b.'_

Colee fli*sa %)

Ropldku talt l i.. amrdf Fito mi r

.. ber hrv.

A-Pa w 0t B=P.

Lt L-s-+Larvae Larva,w19 (.1 )

WeightISievavi.d Me

/.eghtI Catff el Of -

o Welghsit i.1-1W.&Mer h l

ri rMe w

1,dghllt Cad ~

of Petare d.t fe-laati1.

fes laat-(.1)

-dtt (.t0

-A-9 naiuherof hu-e (ait)

SUarni amhuerot I-

'~

ev of i

laamW in 1%)

O 1.4W l...b.

4amhor if

.O oi.

-atrd

(%)

1-m, (0g) hl-.

-l)(%)

l-ve (a.9

`

A 10 10 14.32 22.80 8.48 0.848 0848 8

10 10 14.97 22.15 7.18 0.718 00781

'07 07 0802 8..770.6

.7 072

.72100.0

+

"0.802+

8.7 Not applicable

,.Control C

10

-10 12.74 20,46 7.72 0.772 01287077 D

1 0 1 0 1 4.1 1 2 2.8 1 8.7 0 0.8 7 0 0.8 7 0 E

10 10 14.06 22.82 8.76 0.876 0.876 F

10 to 0 14.05 22.62 8.57 0.87 0+834 650.857 9..

0 77.

-1.4

,G l0 9

15.70 23.34 7.64 0.849 0.764 H

I0 10 14.43 21.98 7.55 0.755 0.753 I

to 0

13.63 21.12 7.490 0.749 22.6%

10 10 14.15 22.11 7.96 0.796 0.796 54

-00.0

,' o.os 54 0.1 K

10 10 12.46 21.00 8.54 0.854 0.854 L

10 10 13.82 21.88 8.06 00.806 0+806 M

10 10 13.84 21.08 7.24 0.724

0.724 10 13.27 21.43 8.16 0.816 0808 7.4.86 100.0 0.808 7.4

-0.7 45..%

O23.17 8.25 0,825 0.825 "P

10 0

13.36 22.01 8.63 0.865 0,865

""_+'

Q 10 10 13.35 22.25 8.9+0 o890 0o890 72.6%

R

.10 10 13.47 21122 7.75 0.775 0134 6.0 0.775 O00.67 0.834

.6.0

-4.0 S

10

£o 14.87 23.43 856 0.856 0,856 100.0,.0 T

10

£0 13.07 21.21 8.14 0.814 0.814

________J___

U 10 10 13.18 20.93 7.75 0.775 0.775 100%

V 10 10 14.61 22.18 7.57 0.757 01835 10.1 0.757 100.06 '

0.835 10.1

-4.1 W

10 10 14.66 24.05 9.39 0.939 0939

ý ;

X 10 10 15.39 24.06 8.67 0.867 U0.867 Y

'0 10 15.40 23.53 8.13 0.813 0813 100% Intake

Z 10 10

£3.16 20.42 7.26 0726 0.772 4+6 0726 100.0 0.772 4.6 3.7 AA 10 10 15.73 1

23.53 7.80 0,780.

0.780 I a 10 10 12.86 20.56 7.70 0.770 0770 outfall 10£;

Dunnelt's MSD value:

PMSD:

Intake:

Dunnett'a MSD value:

PMSD:

I

0.1075 13.4 0.0764 9.5 MSD Mmnimun Significant Differen*e PMSD =

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statiscally significant u a whole effluent toxicity test Lower PMSD bound determined by USEPA (10th percentile) : 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET nhirlabofatoryVariability Sthdy (USEPA, 2001 a; USEPA, 2001b).

USEPA. 2001a 2001 b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluest Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-0I-004 arid EPA-821-B-0I-00S. US Environmental Protection Agency, Cinctnrats. OH.

"S 0

Environlmental Testi ng Solutions, Inc.

TVA /Sequoyah Nuclear Plant, Outfall 101 UV-treated November 17-24, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:

11/17/2009 Test ID:

PpFRCR Sample ID:

TVA / Sequoyah Nuclear: Plant,, Outfall 101 End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Dischargc Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

UV-treated Conc-%

1 2

3 4

D-Control 0.8480 0.7180 0.7720 0.8700 11.3 0.8760 0.8570 0.7640 0.7550 22.6 0,7490 0.7960 0.8540 0.8060 45.2 0.7240 0.8160 0.8250 0.8650 72.6 0.8900 0.7750 0.8560 0.8140 100 0,7750 0.7570 0.9390 0.8670 Intake 0.8130 0.7260 0.7800 0.7700 Transform: Untransformed I-Tailed Isotonic Conc-%

Mean N-Mean Mean Mm Max CV%

N t-Star Critical MSD Mean N-Mean D-Control 0.8020 1.0000 0.8020 0.7180 0.8700 8.727 4 "

0.8153 1.0000 11.3 0.81,30 1.0137 0.8130 0.7550 0.8760 7.672 4

-0.247 2.410

` :1O75 0.8153 1.0000 22.6 0.8013 0.9991 0.8013 0.7490 0.8540 5.374 4

0.017 2.410

'0.1075.

" 0.8153" 1.0000 45.2 0.8075 1.0069 0.8075 0.7240 0.8650 7.381 4

-0.123 2.410 40.1075 0.8153 1.0000 72.6 0.8338 1.0396 0.8338 0.7750 0.8900 5.997 4

-0.712 2.410 0.1075 0.8153 1.0000 100 0.8345 1.0405 0.8345 0.7570 0.9390 10.150 4

-0.729 2.410

"!0.1075 0.8153 1.0000 Intake 0.7723 0.9629 0.7723 0.7260 0.8130 4.648 4

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.939999402 0.884

-0.02446093

-1.22636643 Bartlett's Test indicates equal variances (p 0.91) 1.50255847 15.08627224 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100

>100 1

.0.107485809 0.134022205 0.000919567 0.003978306 0.943894863 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point SD 95% CL(Exp)

Skew IC05

>100 ICIO IC15 IC20 IC25 IC40 IC50

>100

>100 sqnJOJJJ -1 7-O9data-uv

Environmental Testlng Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated November 17-24, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:

.11117/2009 Test ID:

PpFRCR Sample ID-TVA / Sequoyah Nucleai Plant, Outfall 101 - Intake End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

UV-treated Conc-%

1 2

3 4

D-Control 0.8480 0.7180 0.7720 0.8700 11.3 0.8760 0.8570 0.7640 0.7550 22.6 0.7490 0.7960 0.8540 0.8060 45.2 0.7240 0.8160 0.8250 0.8650 72.6 0.8900 0.7750 0.8560 0.8140 100 0.7750 0.7570 0.9390 0.8670 Iniake 0.8130 0.7260 0.7800 0.7700 Transform: Untransformed 1-Tailed Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD D-Control 0.8020 1.0000 0.8020 0.7180 0.8700 8.727 4

11.3 0.8130 1.0137 0.8130 0.7550 0.8760 7.672 4

22.6 0.8013 0.9991 0.8013 0.7490 0.8540 5.374 4

45.2 0.8075 1.0069 0.8075 0.7240 0.8650 7.381 4

72.6 0.8338 1.0396 0.8338 0.7750 0.8900 5.997 4

100 0.8345 1.0405 0:8345 0.7570 0.9390.

10.150 4

Intake 0.7723 0.9629 0.7723 0.7260 0.8130 4.648 4

0.756 1.943

.0764 Auxiliary Tests Statistic

. Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96844363 0.749

-0.33306569 -0.81375687 F-Test indicates equal variances (p = 0.30).

3.80257463 47.46722794 Hypothesis Test (1-tail, 0.05)

MSDu MSDp

. MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.07642232 0.09528968 0.00.1770125 0.003093458 0.478019476

.1, 6 Treatments vs D-Control sqnJO1_l M-7-O9data-uv

............... I....

TVA/ Sequoyah Nuclear Plant, Outfall 101 - UV-treated November 17-24,2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Li Daily Chemical Analyses Environmental Testing Solutions, Inc.

r.Project number:

5812 Reviewed by:

f L

Concentration Parameter Conductivity (tunhos/cm) 332 Co0trol Alkalinity'(mg/L CaCO3)

Hardness. (mg/L CaCO3)

Tern rature C 25.0 24.8 24.9

4.

24.7 24.9 24.8 24.7 24.8 24.8 24.8 24.7 24.7 pH (SU) 764 7.63 7.59 7.39 7.68 7.51 7.63 9

7.18

__DO

_m__/L) 77 76 8.0 3

7.9 7.4 0.8 779 70 11.3%

Conductivity (jumhos/cm) 3100100 Temperature CC) 25.1 24.6 24.9 9

24.9 25.0 25.0 24.8 24.8 2468 248 24.9 24.7 pH

_____(SU)_____

7.63 7.49 7.63H 7*.40O 7.57 7.38 7.69 7.52 7.62 7

.1 53 7.18 7.60 7.18 DO (mg/L)___

7.8 7.6 8.01 7.31 7.9 7.1 8.0 7.7 7.9 72.9 7.1 8.1 7.1 22.6%

Conductivity (pmhos/cm) 299 296 293 2

92 292290_9 Tern erature CC) 25.1 24.6 25.0 24.9 24.9 24.8 25.0 24.8 24.8 2468 24.8 24.9 24.7 pH (SU) 7.61 7.49 7.62 7.37 7.53 7.36 7.68 7.481 7.60 7T6 74 71 7.59 7.15 BO (rag/L) 79 75 80 72 79 7.1

8.

77 79 739 7

8.

70 45.2%

Conductivity (junhos/cm) 26__

576 TemperatureCC) 252 247 250 24.8 25.0 8

25.0 248 8 2424 24.8 247 249 247 pH

______U)___

7.59 7.47 7.62 7.35 7.491 7.34.

7.65 7.48 7.53

71.

43 719.57 7.15 DO 8.0 7.4 8.0 7.2 801 7.31 8.1 7.7 7.8 738.0 6.8 8.1 6.9 72.6%

Conductivity (iemhos/cm)

_20 223 2417171 Temperature C

.252 247 250 24.8 250 250 250 246 248 248 250 246 250 249 PH (SU)

.7.561 7.441 7.57 7.38 7.4 7.301 7.621 7.47 7.511 7.091 7.37 7.16 7.521 7.131 DO____________

8.01 7.61 8.01 7.3 8.01 7.11 8.11 7.8 8.0 7.3 8.0 6.9 8.21 6.91 Conductivity (junhos/cm) 100%

Alkalinity (mg/L CaCO3)

Hardness (mg/L CaCO 3)

Temperature CC) 25 3 24:7 25.2 24.8 25.0 24.8 25.2 24.7 249 247 250 2491 25.1 24.7 DO______________

8.31 7.71.

8.01 7.3.

8.11 7.31 8.21 7.8 8,0 7.2 8.21 6.91 8.21 6.9 Conductivity (punhos/cm) 1 100% Intake Alkalinity (mg/L CaCO3)

Hardness (mg/L CaCO3)

Temperature C) 25.2 24.5 25.0 24.8 25.0 24.8 25.1 24.6 25,.1 24.71 24.91 24.6 24.9 24.8

Cm~~mnmenta luTdin ofues Inc.

Page 5 of 6 Species: Pimephales promelas Client: TVA/ Sequoyah Ni leair Plant. Outfall 101, UV-treated Dally Chemistry:

Date:

Ifays

.11*

I LA

)

Concentration Parameter.

Conductivity 53o)

CONTROL (Amhos/cm)

UV-treated Alkalinity H(mg CaCr 3/e)s Hardness (mg CaC0 3/L) g Temperature ('C)

-LSo 14.1i~.

~

nfl( {ST I.

74.Uq~ 11 'JMM 41 lA.~

PH (SU)

I a

DO (mg/L) 11.3%

Conductivity 1

(P mos/cm) 1~

.0 Si2t 11 2..

M

~lO1

'-'IA Temperature (°C)

Lii. I 4

'LIS -

PH (S.U.)

.22.6%

DO (mg1L Conductivity (iAmhos/cm)

Temperature ('C) 0 nH (S.U.)

4-DO (mWL) 45.2%

Conductivity (A+/-rnh s/cm) 1 Temperature (°C) a pH (S.U.)

DO (m/-L) 72.6%

Conductivity mgrhos/cm) 7-S7 14 A

-00

-I.C)

Vq *3 0100 201 L.0 1S - 1 1610 A

9,40 1 14,(02-

'42 A(44 1S.

VA-1 15.0 0

q.(07-t4cl E). C)

BIB U3

'LIS

.0 I.qq 80

8. o 101 lot 171 68 4 0.1 TS -Pý

ý [

1 4,qq I I.qq 11 74 1 14. kn 11 1.151 Temnerature (MCY

H p-s.U.)

Conductivity.-

(tgmhos/cm)

Alkalinity 100%

(Mg CBCO 3 Hardness (mg CaCO3/L)-

fb'WIt*ftfb TR chlorine (mg/L)

Temperature (C I

I I

DH (S.U.)

DO (mgWL)

II k100%

" Intake Conductivity

/~

Alkalinity

.Co-(mg CaCO 3/L)

Hardness (mg CaCO3IL)

TR chlorine (mg/F) "

1

.1 0.10 II Temoerature (. C)

"S.2. -

m b.U ii II.,&

H.'

L'4*

I iTemperatur I -(Initial ii Initial Final Initial I Final

]

SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 58 of 102

r

~)

Env~roi~mgnt IuTfngShlon.,

Inc.

Page 6 of 6

.Species: Pimephales promelas Client: TVA./ Seau6vah Nucleai'Plant. Outfall 101., UV-treated Dae:

iI--

Dav I

An'Aalyst L

6 Concen-Parameter,--

pH (S.U.)

DO (mgh/L)

Conductivity (Mmhos/cm)

CONTROL UV-treated Alkalinity (Mg CaCO3/L)

Hardness (me CaCO,/U*

(Mg Ca03/L, 4 _LIM Temperature (MC) l-pH (S.U.)

i~1 1-DO (mg1L) 11.3%

Conductivity I(gtmhos/cm) 4,U v

.Ig Temnerature ('CQ

-V 1A-A

-A -

I pH (S.U.)

'-t~ I

.C57 1 7.1n2.

11-,

(

I 1.V?

~.-.T I-DO (mg/L)

~LO 22.6%

Conductivity Df.

33

`10 rýIq I

4tn

'II 0

._1~

Temperature (;C)

U P ~

PH (S.U.)

DO (mg/L) 45.2%

Conductivity I

(grnhos/cm)

L~~-O i7.

L a~

0) 4

':~.,5-I i'{~

I.5 II4L)1 I 4-~

I'%

~

65'7 Temperature (0C)

P.

oH (S.U.)

.4-3 1

ý 2ý DO (mi/L) 72.6%

Conductivity (gmhos/cm) a~ll1B

'-Ms0 VUI

-LA. a

_QL. n

-LA. %0

-Ls. n i%1.1 Temperature (0C)

I 0.11MMIN111111M

..... i

-M pH1 (S.U.)

/T t

- 1.41i I

.,l Ii-c

.(o I ",2.

II H,1.

I P

DO (mg/L)

100%

AM-A'eMPb-Conductivity.

(gmhios/cm)

Alkalinity (Mg CaCO3/L)

Hardness (Mg CaC0 3/L)

TR Chlorine (mg/L)

Temperature (°C) 8.0 1

ILD~

40A. to

1. -L. 2-ilq

--I.,

pH (S.U.)

"7. cG I:

DO (mg/L) 100%,..

Intake Conductivity

-(Amhos/cm)

Alkalinity (mg CaCO3/L)

Hardness

'(Mg CaCO3/L)

TR chlorine (mg/L)

Temperature (0C) 8o' I OaO Loot.

II

-14C. I II (.4. (a

-M I-I Initial m

Final I

Initial ll I

II I

II SOP AT20 - Exhibit AT20.3, revision 04-01-09 Page 59 of 102

Page 3

Page__tof I 0

EnvlronmentalTestlng Solutions, Inc.

Total Residual Chlio'en.ne (Orion Eletrode Method, Orion 97-70)

Matrix: Wateir

. =

lO g Meter:'Accumnet Model AR25-pH/1-on Meter.

Date aaye cdegn Calibration:

0.10 mag/L 1.00 must a Slope e

Reference standard number I qt, "15S JS Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV X 100 number (mg/L)

(mg/L)

(acceptable range = 90 to 110%)

I N5SL&?

0.50 Ov4 2..

Duplicate samp"le precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD

{(S - D) /[(S+D)/2}) x:100 nube (al)(acceptable range =

10%)

01)"11-1. OA btA~icu l-c-CF Llm~xrt. color S0.31 Duplicate D o.oIq0 Sample measurements:'

Sample Sample ID Sample characteristics Residual chlorine number m

b Reagent Blank

LOOoBL, o0% 1111.

Jtb' IN

- Wj Clef -NO C0 I QV; f PIo.41-el8 60,111-1-0

. dcovJSO paj(+) r3 0.0W

'L~OL6fl O'M16.02. V n F-1Z*4T -IA\\iaclor cak..

a t-o0. 0040 3 Oct IN, -.

1

'nT-W DSN1\\J-1k 001-1; Sigittycowij I pa It-4-aN OO25

-. J I

"=.0 1U GOW1thi.S S,

lý-(

~

ci/~Caliaes lcak +ONi O0o002-00 M ilt i0.6 %N

_._-_10:

T0-/'

Znrt__.-

0.0011_7 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratory control standard:.

Reference standard True value (TV)

Measured value (MV)

% RS = MV /TV x 100 number

" (mg/L)

(mg/L)

(acceptable range =90 to 110%)

Z445 61 0.50

0. LAI1 C)- 4J Reviewed by I

Date reviewed _

1.-1 jJ SOP C8 - Exhibit C8. 1, revision 09-01-09 Page 60 of 102

I Page 5

Page of L.

F.nvlronmentalTestlng Solutions, Inc.

Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

Matrix:.Water,RL-= 0.10 mg/L Meter: Accumet Model AR25-pH/10n Meter fos t

Aid ýreagent:

ýQýj Date~analyipd:

A odde rgnt:Tg.3 Calibration:

0.10 mg/L, 1.00 mu/L b Slope l

Reference standard number 3

.I Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Lahnroinru innfrni.eiandard~

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number (rag/L)

(mg/L)

(acceptable range = 90 to 110%)

-*0l 0.50

o.

I02..0"I.

Duplicate sample precision:

Sample, Sample ID Sample characteristics Residual chlorine ' %RPD = {(S - D) /[(S+D)/21) x 100 numbe (mL)

.(acceptable range =*10%)

number..

S 10%)2 Dupic D

ato.oo1e Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine numberb e

Reagent Blank L0.OVf o)e l:"

I

',hmr\\gtoN V*XTQ Lo.w32.

ocuL 5

t35V

-IOV-T0 /,-e//

oI_[x,*oocoaor

,.oloO5 OrI16,0' 5*0*;A N-i

.TO*,

uck

,oc Coiteoir tJ,ooqic it it

,,x CX31 C1 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratoriy control standard.

Reference standard

[

True value (TV)

-Measured value (MV)

% RS = MV / TV x 100 number (mg/L)

(mg/L)

(acceptable range = 90 to 110%)

tr-.rs' Q13 0.50 I.So 100.14 Reviewed by I

-3.

O*

Date reviewed

_11.

Iq.

SOP C8 - Exhibit C8. i, revision 09-01-09 Page 61 of 102

Page__ 7 Page I ofj SS ETS--

) Environmental Testing Solutions, Inc-Total Residual Chlorine (Orion Electrode Method, Orion 97-70)

Matrix: Water,.R = 0. 10 mg/L Meter: Accumet Model'AR25 pH/Ion Meter de r

l.

.I,

.,maiys:[.::L~

l*)

" ] ': , -

',::Iodide rdagent:-t.

[.

-ADate an dcid reagent.: 1'=N2 Calibration:

Reference standard number of

> 10 ra/L h.00 r

ge S

lop e Note: Far samples with a residual chlorine of > 1.0 mg/L. the-calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number (mg/L).

(mg/L)

(acceptable range= 90 to 110%)

_,Ss~rI 3 0.50

o. l Duplicate'sample precision:

Sample.,

Sample ED Sample characteristics Residual chlorine

=RPD

[(S - D) /[(S+D)/21) x 100 number (m

(accetabl range =L,10%o)

Duplicate~

__3CP Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number (mglL)

Reagent Blank O.

M Im1.

0~

5W&S, cP\\IO jCqAOc

~

J noAt V N CAC II pck1d tc,.oooEoq CA

-n (M

L1

+-.

_-X -Ts4rer Cim

.d tau 0'iii.

Ift T'P-RW-r-C.

yr'~

wbputiduec. p k +cirN 000213 0iw Im 06 f tAF rrv-Cot C.

Laig 1&o+Or,4

082, 05 1 IzLo !. cS Jý 5x-Zk~ b__________

e.M))o pO0lo-OA' Samt.- Io(-Tcl*' A clI-eO.xv moz COIoy CLI Nrote: All samples were analyzed in excess of EPA recoimmended holding time (15 minutes) unless otherwise noted.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS MVITV x 100

- number I(ung/L)

I(mg/L)

I(acceptable range = 90 to 110%)I qgýL J'LSýAm0.50.

0.4796 Reviewed by Date reviewed I LI 0 SOP C8 - Exhibit C8. 1, revision 09-01-09 Page 62 of 102

EnvftoiMlUtIal TIuing SolutIonh. Inc.

Page l(p Page of_-

Analyst Date.analyzed

(

Titrant normalitw'and multlilier determinj Alkalinity (SM 2320 B).

Matrix-.Water, RL = 1.0 mg CaCO31L Titrate samples to p H =

P 4.50 S.U. ;.

Time initiated 06&-'L Time completed ONj pHo.of Normality Normality (N) of H0S" pH Factor or Multiplier.C-Deionized Titrant check Begin End Total

= (5 ml NazCO3 x 0.05)/E

= (N x 50000)/100 ml sample water "reference standard ml ml ml

`- 0.25/E

=N x 500 4.5 S.U.

number number (E)

(acceptable range = 0.0180 - 0.0220)

-4.5 S.U.

~

nu be nu m ber13 7__

- 0..f L Z o -

caND on o ~nitajrd; Reference standard True value Sample.

Alkalinity (MV)

% RS = MV / TV x 100 number

. (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (Ing CaCO3/L)

(ml) ml ml ml 90 to 110%)

1"00.1 100 Oz.o 2.'s C1.5" iO.b qo clO'.

Duplicate sam le precision:

T Sample Alkalinity

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S -D) /j(S+D)/211 x 100 number (ml) ml ml ml (acceptable range =.:k10%)

tll-ar,*

0a%1zLO tD,

AS A.4 61' 10.,4 s

Duplicate (B)

LgL.q 35.3T59 4

D 0

Matrix spike recovery:

Reference standard Spike'value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3 /L)

(mg CaCO3/L)

(ml ml m

ml

-00 I.

36 0o0.4 110 Sample alkalinity (B)

Measured spike value (MV)

%.R = MV / SV x 100 (g

CaCO3/L)

MV = A - B (acceptable range, (mg CaCO3/L=

75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml ml

-i ml Multiplier.

(mg CaCO3/L)

I-t -1Lt\\A8 NKS

-o 0

310.1 4 4.2' U.0 LOA (a?-

11.IL1o0 4

0,o0 5

.5 to 11-09-0,9

'5-b -StO.0*31.4 46A 10-3.I10 31 0 i* it rL..t '

a s P ?-

TP q.5

,C1.5 o.2.

2,1t n, It 1%

-of a*-a.,

_ p.

60 10.*,*t.6' (2) 2.70 ci,,t o.o--

Fb,,ý.* wj 110o A0o

AP' A.-t

-1 x-4 ae 0-

~

Reviewed by:

I Date reviewed:

-1$*8'-.

Page 63 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09

I i

ivmnmental Tesdng Soutia

, ine, Page R_

Page 2-of *3 Matriý Analyst 1i:v Date analyzed.I i

1111 TL,..:-."irnltv and multiDlier determination:.

Jkalinity (SM 2320 B)

Water, RL = 1.0 mg CaCO31L Titrate samples to pH =4.50 S.U.

Time initiated Time completed f'Noirmality

()

of H2SO I

pHFactor or Multiplier:,

Deionized Titrant check Begin nd-(- ml Na2CO3 x 0.05)/E

= (N x50000)/ 100 ml"samplet

..water re.eference standard ml ml ml I V

=N 500

- 4.5 S.U-number number

_____E)

(aEceptablerang = 0.

0 Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS MV I TV z 100 number (TV).

volume Begin End Total Multiplier (mg CaCO31L)

(acceptable range (mg.CaCOdL)

(ml) ml ml_

ml I

=90 to 110%)

100 1oo q.s6-

.3' bL -

Duplicate sam le precision:

Sample Alkalinity

%RPD =

Sample Sample ID volume Begin End Total Multiplier.

(mgCaCO3/L)

((S - D) /(S+D)/21) x 100 number J

(ml) ml ml ml (acceptable range =- 10%)

Duplicate (B)

D K0 Matrix soike recovery:

na+- eAro~ 4jciJvAqI-' 24%tVldAeAe p

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)

I(mg CaCO3 1L)

(ml)

I+/-l m

ml I 1A5S__b-*q 10 1r o

.-+

ý.*

0.3 t.tto, Sample alkalinity (B)

Measured spike value (MV)

% R =MV SV x 100 mI (g

CaCO3.)

I MV = A - B (acceptable range I

(mg CaCO31L)

= 75 to 125%)

ILO 100.0*

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample [D (ml) ml ml ml Multiplier ni1 CaCO3/L) i)

13,0

,C.1X.1 1.3

.4 O.q JW**g, tr,..3, q.6 0.6 "x

4.R",

'S

/

Io, 12.-

049

/G3-L*tRiZM E--d*-*

i-t.q 1?.0

,.S o

NLI-)o VWIN Ti 1,.0 I.sm I

\\Ot'5

'7.

l1~

0~oZ U0__51 425

.A Reviewed by:

zI -

Date reviewed: I A-I*

Page 64 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09

I SE-nvironn,"fi T. ng Soluin, Ionc..

Page 18 Page--

-Of.5

Analyst F~C Date analyzed,-[iii~ I Alkalinity- (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Time initiated Time completed Titrate samples to pH = 4.50 S.U.

Titrant norm i~a Dwjk~ate sam ie precision:

"! Ie A lkalinity

% R P D -

Sample Sample ID volume 1

End Total Multiplier (mag CaCO3/L)

{(S - D) /f(S+D)/21) x 100 number

"_(mi) mi_

(acceptablerange_

10%)

Duplicate (B)

D Matrix spike recovery:

eren-value Sample Spike alkalinity (A)'

number (SV) vourE-"Begi--

Total Multiplier

. (Ig CaCO3IL)

(rag CaCOAL)

(ml) ml mi mi-Sample alkalinity (B)

.. Measured spike value (MV)

% R =MV / SV x 100 (mg CaCO3IL)

MV = A - B (acceptable range (mng CaCO3/LI

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total.

Alkalinity Sample number Sample 1ID (mi) ml ml ml Multiplier (mg CaCO3/L) le.- 4 A93 118.

i oqLtfo.oI J

k-j 2-\\

3,t6 3q. 5-~

, zi ocilIO..

64_v CAilO 11~ 17-01_

L2 Page 65 of 102 Reviewed by:

I AI1 Date reviewed: I it.- 0is SOP C6 - Exhibit C6. 1, revision 06-29-09

P En~lmnme Iotn s, Inc.

Page 2-1 Page 1

of_______

Time initiated 0 ro Time completed I

i2.

- Analyst 6SL Date analyzed Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.

Titrant normality and multiplier determination:

I Duplicate sam le precision:

F.

Sample Alkalinity

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CatOWL)

((S -D) /l(S+D)/21) x 100 number (ml) ml ml ml (acceptable range = 10%)

Matrix spike recove ry:-

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume.

Begin End Total Multiplier (Ing CaCO3I-)

I(mn CaCO3 1L)

(ml) ml nt,.

Sample alkalinity (B)

Measured spike value (MV)

% R = MV /SV x 100 (Ing CaCO3/L)

NMV = A - B (acceptable range (mg CaCOIL)

= 75 to 125%)

Sample measurements:.

Sample volume.

Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)

I1-2L.05 M114-S H -.

o

k.

II-a 9

.3 I

._ke_____2-

_t4O 0I.

o 7-1.

9 110

.0 2cI O041116 O&

1 Q-4

.at.4 I_"

z.

-L 0__ r.

2i Reviewed by:

Date reviewed:

i

't"c.'O Page 66 of 102 SOP C6 - Exhibit C6.l, revision 06-29-09

U r*

Page R

Page.

ofZ5.p

,.;,Analyst Date analyzed Titrant normality and mult*iier determination_

Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH = 4.50 S.U.

Time initiated Time completed !

iip 4

4pH Factor orMultiplier.',

E (Nx 50000)/ 100 ml sample N x 0500 p

., Nomlt (N.f 2 5

.Normality

-Deionized Titrant check

- - Dchu water reference standard ml ml

-4.5 S.U.

number number

-TAUL

" Normality iN) o HSO4

= (5 ml Na2CO3 z 0.05)/

(acceptable range = 0.0180 - 0.

iM j

. u

1 1-1 I

U I

I I

S I

I I

I Laboratory control standard:

"Reference standard True value Sample Alkalinity (MV)

%RSMV/TVXI10 number (TV) volume Begin End Total Multiplier (mg CaCO3-L)

(acceptable range (mg CaCO3 /QL (ml) ml ml ml

-go

='

to 110%)

I,ýS q,.

100 100

__ 5.00 10_____.-_510o__

Duplicate sample precision:

1Ak tD ISampleAlaiiy0RP Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L)

{(S - D) Il(S+D)/2)} x 100 number

-(ml)

M ml ml (acceptable range Duplicate (B) 14+5 9

-k,-

1 1Iog Matrix spike recover:.

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mag CaCO31L)

(mgCaCO/QL)

(mI) ml -

ml

.il J_1_

5 1 0 4 S Z - f S 1 0f o i i Sample alkalinity (B)

Measured spike value (MV)

% R MV/ SV x 100 (mg CaCO3/L)

MV = A - B.

(acceptable range (Mr CaCO3jL) 75 to 125%)

Sample measurements:_________

Sample volume Begin End Total Alkalinity Sample number Sample ID ml) ml.

ml ml Multiplier (mg CaCO3/L) o,*1 60

ý..*

i'-P5 on D

4.A Z2.q

-5.1, I*.*£ Izo

-3 5

lc ~~i

-~~.4 1 WAe 00ZV 6U..

412 2 11, 5ID

.,o S.13)

,01

-5.3 11,0 5-1 12,0 2 not\\1

.oS I,

AI 5k

-1 Lo)

_t,,

.a-___

a4 7 U1 Ul~

4

~

4

,Reviewed by:.

Date reviewed: Iil-L

_'" I I

Page 67 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09

S 9

9DETS

~

eootronn,.n aIT.otlvg So u 015 *~C Page Page Ofz*.J_

Analyst 5,

.Date analyzed.

I i IIII]

Alkalinity (SM 2320 B)

Matrix: Water, RL - LO mgCaCO3/L Titrate samples to pH = 4.50 S.U.

Time initiated Time completed r, Vý Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS = MV / TV x 100 number (TV) volume Begin-End Total Multiplier (mgCaCO3/L)

(acceptable range (mg CaCO 3/L)

(ml) ml ml ml

=90 to 110%)

z-,

100 1o0 d4.

oz.

02 Duplicate samn le precision:

Sample Alkalinity

%RPD Sample Sample ID volume Begin End.

Total Multiplier (Ing CaCO3/L)

((S - D)/[(S+D)/21) x 100 number (m_ l ml ml ml I I (acceptable range =* 10%)

3 S

q

5.

394 fl4 2

Duplicate (B)-

Djyj10, Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Ing CaCO3/L)

(mg CaCO3/L)

(ml 4

)

ml ml ml

-too J qv

'A),.

a(i) o

/I,O Sample alkalinity (B)

Measured spike value (MV)

% R = MV/ SV x 100

.(mgCaCO3/L)

MV=A-B (acceptable range (mIg CaCO3/L)

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number SampleID U)

(l.

ml ml ml, Multiplier (mg CaCOs/L) o4 11 rl If 1

60 6,0 34I 3,1 12U104 0i 1

.t04 7

L.

(

J'o 011,11

-0S-3.

&A 2.9.1 orlll.. S to io k' 1

17,0 3.2 CA.i J.LQ1,Q dA,..

2-0, U_'

-3

___0 A-1

-61_

Q~iLLVASCL IL 6

) 1rtw I

-o

.3~.1 (I(

0,1iJ 17-.

us_

3&.

1ý

_____,_1 olItlO, oS 3

o, 3,o(.,

.Reviewed by:

Date reviewed: I I\\v2Z-1 0*

Page 68 of 102 SOP C6 - Exhibit C6.1, revision 06-29-09

6 0

0JETS

~

En~ ronmrnta Twing Page -4 Page

_*of Time initiated Time completed Analyst Date analyzed

.t O

Titrant normality and multiplier determinatloi Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH 4.50 S.U.

water S4.5 S.U.

I

-,'Normality'.

n.

'- check Begin number number S.

.Normality (N) of HSO End Total

= (5 ml Na 2CO 3 I 0.05)/E ml ml

--0.25/E (acceptable ranee = 0.0180 - 0.0220) pH-Factor or Multiplier (Nx 50000)/ 100 mnl samp'le

= Nx 500 T.

Laboratory control standard:

Reference standard

True value Sample Alkalinity (MV)

% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (mg CaCOgL)

(ml) ml ml ml

=90 to I10%)

-f3t4.

I100 100

/0I3 0,,

Duplicate sam le precision:

Sample

. Alkalinity

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

[(S - D) /[(S+D)/21) x 100 number

_ml) ml ml

. ml (acceptable range 10%)

IS

____Duplicate(B 0

H Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) -

volume Begin End Total Multiplier (Ing CaCO3/L)

(mg CaCO3/L)

(Ml) ml ml ml Sample alkalinity (B)

Measured spike value (MV)

% R =MV I SV x 100 (Ing CaCO3/L)

MV = A -

.(acceptable range (Ing CaCO3/L)

=75 to 125%)

Sample measurements:*

Sample volume Begin End Total Alkalinity Sample number

-Sample 0ID (ml) ml ml ml Multiplier (mg CaCO3/L) o 011 lgA. o -Z1-6,Qn 21 L1'(

7J q_ 14)

C).-+

4 2,o q7, 0__

1 o,

.IL9.

ie.1, i

io 01 ',

l-7-

0117._. o % *03 16_,0 A t 0 IL41 to eiw I JL

30. 0 Page 69 of 102 Reviewed by:

Date reviewed:

SOP C6 - Exhibit C6.11 revision 06-29-09

F nvironme,,

alTemngSoludons, Inc.

Page *5 P age o f _

Time initiated Time completed Analyst. -+< i4 j,,]

'Date analyzed I..

,Tltr4 Mff~

.v "er deterrainattot Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.

U -

a -

a Deionized.,Titrant water reference 4.5 S.U.

number

Normality.

check.

standard number

.,;;,Normality.(N) of HiS0 4

.-m;,pH.Factor orMultiplier:

Begin End-Total azCO.sX0.05)/E

= (NxSOOOO)ll00mlsample ml ml ml V

=Nx 500 (EM (acceptable range = 0.0180 - 0.0220) 11

,L

-L Laborator control standard:

Reference standard True value -

Sample Alkalinity (MV)

% RS = MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (g

CaCO3/L)

(11) nil ml ml

-90 to 110%)

2 S -

100 too 31.8 11 j 53 10.1

100, tOO'/.

Duplicate sap ple precision:

Sample I.*

Alkalinity

%RPD =

Sample

, Sample ID volume Begin End Total Multiplier

'(mg CaCO3/L)

{(S - D)/[(S+D)/21} x 100 number (ml) ml ml ml 1!

(acceptable range = 1 10%)

W IZI W) 2.

100 hqA,.

31.4 0-2 C

Duplicate (B)

D5 tej j

D Matrix spike recovery:

Reference standard Spikevalue Sample Spike alkalinity (A) -

number (SV) volume Begin End Total Multiplier (mg CaCO3IL) r (mg CaCO 3/L)

(ml) ml ml Sample alkalinity (B)

Measured spike value (MV)

% R = MV /SV x 100 (mg CaCO3/L)

MV = A - B (acceptable range (In CaCO 3/L) 5=

7 to 125%)

zO IOo 1-Sample measurements:

Samlple Volume Begin End Total.

Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) 4 2 -

3 6 1.0 0

(

4A S 364 qo,oq.6 o.S Reviewed by:

Date reviewed: I c

Page 70 of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09

)EnvlronmentalTestlng Solutions, Inc.

Page Page (0 of Time initiated Time completedI.1III_ IIL Analjyst%

Date analyz~ed l

Alkalinity (SM 2320 B)

Vdatrix: Water, RL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.

-,_,Tltrant normality nd multiplier determination:

Deionized-TtRitrant check

Begin - E water e"e standard ml

-4.5 S.U.

'0e, number' U

I nd Total ml ml I1R S-Normality'(N) of H 2SO 4: -'

=,(5 ml NaiCO 3 x 0.05)[E

= 0.25/E (accentable ranee = 0.0180 - 0.0220*

pHFactor or M6ltiplier-1

= (Nx 50000)/100 ml *ample S =Nx 500 numb_______________

I (acceptablerange_=_0.0180_-_0.0220)

Laboratory control standard:

Reference standard True value

.lwe Alkalinity (MV)

% RS = MV / TV x 100 number (TV) volu Begin End Total Multiplier (mg CaCO3IL)

(acceptable range (mg CaCO3/L)

(ml) ml ml ml

=- 90 to 110%)

100 100 Duplicate sam le precision:

Sample Alkalinity.

%RPD=

Sample Sample ID volume Begin End Multiplier (mg CaCO3/L)

((S -eD)l

[(S+D)n21) x 100 numer ____________________

_Val__

(acceptable range 10%)

Duplicate (B)

D Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOsIL)

(Mg CaCO3/L)

(ml) ml ml ml Sample alkalinity (B)

Measured spike value (MV)

% R = MV/ S 100 (mgMV

= A-B (acceptable rangeb, (mg CaCO3/L)

=75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID

-(ml) ml ml ml Multiplier (mg CaCO3IL)

_taolA Lf~$

t) 44 L(2

.M.

-I JL L

(

11 M 6__1 1

3_"

36_16 5;-Y Reviewed by:,

Date reviewed: I i-o9 Page 71. of 102 SOP C6 - Exhibit C6. 1, revision 06-29-09

0 S

0I~ETS

~

Env mn~nta Tat ig So u on. nc Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Page

["__'

Page C

of Time initiated Time completed t ed Analyst _

v)I l.%

Date analyzed Titrant normality and multiplier determination:

Titrant Normality check Begin End Total' Normality (N) of EDTA-pH Factor or Multiplier.-

,reference standard ml......

ml ml 0.2/E

= (N x 50000)150 ml sample' number.

number (E)

(acceptsbit range = 0.0180 - 0.0220)

=Nx 1000

'l t

')!,Z-iA&sstl'k 0,o 10-0 i10,0 7~z z Laboratory control standard:

Reference standard True value Sample Hardness (MV)

% RS = MV /TV x 100 number (TV) volume Begin End Total

-Multiplier (ing CaCO3/L)

(acceptable range (rag CaCO3L)

(ml) ml ml ml

.=

90 to 110%)

j

[,SS7'02.

40 50

%O,0 t12-,.3 7..0

'A 0*.

1O00.0"1, Duplicate sam ple precision:

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (rag CaCO 3/L)

{(S - D) /[(S+D'2jl X 100 number (ml) ml ml ml rx o

'il HS)

NNS01 6

12,a)

LA.'*

20-0 S

Duplicate (B)

1.

5 D

C10 Afatrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)

(mg CaCO3/L)

(ml) ml ml ml 4*

o I-leo Sample hardness (B)

Measured spike value (MV)

% R = MV/ SV x 100 (mg CaCO3/L)

MV = A - B (acceptable range (Ing CaCO 3/L) 75 to 125%)

IOo oo:o.

Sample measurements:

+-

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)

TV= ND Blank (should be =0 m CaCO 3/L) 6_o v__) 0o 7,_0"0 I 1 3 O'~*~(~s W6z

___S

'a

'-S

-IS-___

I v.

.A4 A"

52.O 3:_-_

0,1__

_0rr1.

iS 4z~z 0,3.

6.0

.',Oat -t..k Ee

?-la 00 N)~

390i:.

611IMIbll-M "I ill

,,0Z 3A A__

%La7-183 Pigs 2 172V1JiMF trant is used, sample must be diluted.

Reviewed by:

CAis bate reviewed is -09 SOP C7 - Exhibit C7. 1, revision 06-29-09

0t 0 *,

Page 16 Page 2-of2 Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Analyst [

uIx.I Date analyzed-[.1L

.0Ih1 Time initiatedI Time completed 7

1 FI., a g.

alit and multiplier determination:.

I!

Ttrant.Normality eh-e'

-Total

.Nrmlit (N)ofEDTA pH Factor 6r Muitipiiei reference standard ml ml m1

= 0.2/E

= (N x 50000)1 50 msi-ample i number number (E)

(accepta

- 0.0220)

= N x 1000

-Laborator control standard:

Reference standard True value

Sample, Hardness (MV)

% RS MV /TV x 100 number (TV) volume Begin End Total Multiplier (01g CaCO 3/L)

(acceptable range (mg CaCO 3/L)

(ml) ml ml ml

=90 to 110%)

I As S"b..0 50 qj 2,0.

10.

40 10),01'-

Duplicate -samle precision:_____________________

Sample Hardness

%RPD=

Sample Sample ID.

volume Begin End Total Multiplier (mg CaCO3IL)

((S - D) 4(S+D)/21) X 00 number (ml) ml ml ml 12

_.o Z.

'5 s

to Duplicate (B) 1 12.5, 1.0, C D

10 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mg CaCO3 /L)

(ml) ml ml ml Sample hardness (B)-

Measured spike value (MV)

% R = MV / SV x 100.

(mg CaCO3/L)

MV = A - B (acceptable range (mg CaCO3/L)

= 75. to 125%)

tO 4c) too-ol.

Sample measurements:.

Sample volume Begin End Total Hardness Sample number Sample [D (ml) ml ml ml Multiplier (mg CaCO3/L)

TV Blank (should be = 0 mg CaCO31L)

,0

1.

1,.2- 00 0..

o

" lU-Wk'i'°\\W, Sh,,,n 16-3 in.L 1.I

-,.1, i

I I

I I.

pý*,:'*3"kWY6trant is used, sample must be diluted.

Reviewed by:

Date reviewed i

IJ SOP C7 - Exhibit C7. 1, revision 06-29-09

0 S.ETS 9

~nnitwtntwtei Testing Page I0 pTotal Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Page _

_of *"

Time initiated 17~l7 i

i77I Time completed I

Analyst 6S7L Date~analyzed

.O

.Ttrant normally and.multiplier determination:

Titrant-Normality-check..

Begin-End Total

!-Normality (N) of EDTA pH Factoror.Mu!tiplie4*,_:`.I reference standard ml ml ml 0.2/E

= (N X 50000)/50 ml sample number number (E)

(acceptable range = 0.0180 - 0.0220)

= N x 1000

&2c [JS 1.7O

,LI I

Laboratory control standard:

Reference standard True value Sample "

Hardness (MV)

%RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier r(mg CaCO3IL)

(acceptable range (mg CaCO/UL

(.mn I

ml ml ml

=90 to 110%)

(rJ5S 7-02.

40 50,

0.

q 1o..

q

'0.9 Duplicate sam ple precision:

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)"

{(S-D)/I(S+D)/21) x 100 number (ml) ml ml ml

Duplicate (B).I w

4 ID Matrix spike recovery:.

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mag CaCO3/L),

(myCaCO/L)

(ml) ml ml ml Sample hardness (B)

Measured spike value (MV)

% R = MV /SV x 100 (mng CaCO3/L)

MV = A - B (acceptable range (Mg CaCO 3/L)

= 75 to 125%)

Sample measurements:

"Sample volume Begin End.

Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier' (m01 CaCO3/L)

TV =ND

" Blank TV N

(should be 0 mg CaC0 3.L)

So____13,_____

t14 tl-yl.s WWH t

13,,,.o Z4'-.

1 4.1 1

'84 MI-0~9 GSL~j A.

~ 2-30.0

-A F\\W V.)

,>-.y, 4-.2.

6 SIII I

PaWe 1 t §,Awirant is used, sample must be diluted.

Reviewed by:

-Ik Date reviewed 1 1iI2Z-SOP C7 - Exhibit C7.l, revision 06-29-09

Page "o

Page_

of

'V'~U Tr.~J

-~

IC'1IE ~i~Afl r~\\

I otall Hardness talvi 4340 Q) 77: 7 RLO*.0 mg CaCO3/L S

-i "Time initiated' Date a.a..

i, z

Time complet:ed r...t*..v rmal** v

andmultiplierdetermination:

".*irmi**.

ite

.End Total.

Normality (N) of EDTA pHFactor.orMultiplier reference standard

.0.21E

( & 50000)/ 54it sample number.

nlUllm~ e.*

laccin a-0.20

.N 1000*v Laboralor, control standard:

Reference standard True value Sample Hardness (MV)

% RS= MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (mg CaCO/)

(ml) ml m

ml

=90 to 110%)

Duplicate sample precision:

Sample Hardness

%RPD Sample Sample ID volume Begin End Total Multiplier.

(mag CaCOIL)

(S - D) /[(S+D)/21} x 100 number (ml) ml ml ml AS 0"i I 20 - o Vefr-p1,

10 n4.

I

-l At 4

~

S G bC-66 Duplicate (B) 4

, *.D tg0 3

I Matrix spike recove ry:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Mg CaCO3IL)

(mg CaCO 3/L)

(ml) ml ml ml

s*.

.;°

o..

.L,*- 0.

6

(.5),44 16-o Sample hardness (B)'

Measured spike value (MV)

% R = MV / SV x 100 (mg CaCO3/L)

MV-= A - B (acceptable'range (mg CaCO3/L)

= 75 to 125%)

Sample measurements:_________

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml

.'ml Multiplier (mg,CaCO 3/L)

TV =.ý Blank Z

je 41 140

_1__i_

_ 3 0Aý 10.O 3-

)

4 5A 34

=~-I.+

I51 (31%mg-L iAL RF 002-o t'?A

. a nn

-9

0cj, It it 0fiffj I"

U I*~

j).%

I qq 1

oai% it.

it)

Rig I 7_1 B

I LO

0O1.1%&

5 4

0 Jno 5 bef~ W/ ant is used, sample must be diluted.

Reviewed by!

i t

.I Date reviewed iE l:I SOP C7 - Exhibit C7.1, revision 06-29-09

,FT5 Analy.st i

.Date an -alyzed- -ii oI I]-

Page ZI Page 3

qf 1 -4 Total Hardness (SM 2340 C)

RL =.0 nmg CaCO3/L Time initiated Timecompleted Laboratory control standard:

Reference standard True value Sample Hardness (MV)

% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOA/L)

(acceptable range (mg CaCO3/L)

(ml) ml ml ml

=90 to 110%)

a=s*_40 50 1.5 1-45 1-.:i.5 r+-

Ca. 1 Duplicate sam pie precision: '

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L).

((S - D) /[(S+D)/21} z 100 number (ml) ml ml ml Duplicate (B)

D T

I -

)

-c,4 05 Matrix spike recovery:

"3,,

Reference standard Spike v'alue Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier

'(mag cacoy/l.)

(mg*CaCO 3f/L)

(ml) ml ml ml Sample hardness (B)

Measured'spike value (MV)

% R = MV / SV x 100 (mg CaCO3/L)

MV= A - B (acceptable range (mig CaCO3IL) 75.to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO3fL)

'Blank

=0 (should be 0 mg CaCO3/L) 0(*\\

f.t*j In -r

_.45

_,,o_

0

12) o,

,-4, i

z UU

.a1z.

,v,- l

_____o__S*

.1

,.a*g

,-f3*.'

JI..

to 0______O-_0 3

____o_

q U'3 01I1111.65.

1(tA*So 161-i~ y U, 1 c

CA4 1 0:1

-4 3-3 jaI oit2.~3

-#ý2_

CP 5_ _

PA":Jr6l-*IYJtrant-is used, sample must be diluted'." Reviewed by:, J "

Date reviewed

(*

I

.SOP C7 - Exhibit C7.l, revision 06-29-09

S 0

0S 3ET~

~

Eny ronmen a Test ng Ions nc Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Page QA Page

'4 of 4 Time initiated Time completed Analyzst [7-,-

11,-

Datiiahalyze~d i1 I]

.....Titrant normality and muitinlier determination:

kiii.ý L*gin L-n Total..

..Normality'(N) of EDTA pHýFactoror Ma1tiplieý!ý:ý"

/*"

.-reference standard ml ml ml (N x 50000)150 ml sample number number (E)

Socpal ane008 0.0*lF-"-'---N 1000 Laboratorn control standard:

Reference standard True value Sample Hardness (MV)

% RS =MV /TV x 100 number (TV) volume Begin End Total Multiplier (Ing CaCO 3/L)

(acceptable range-(rag CaCO3/L)

(ml) ml ml ml 90 to 1t0%)

Duplicate sam Je precision:

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (fag CaCO3/L)

((S -

)[(S1D)/21} I 100 number

_ml) ml ml ml Duplicate (B)

D Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Ing CaCO3 /L)

V

"(mgCaCO3/L)

(ml) m[

ml ml

'Sample hardness (B)

Measured spike value (MV).

% R = MV ISV x 100 S

(Mg CaCOi/L)

MV = A - B (acceptable range S

"(mg CaCO31/L)

=75 to 125%)

Sample measurements:-

-Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)

TV =

(slak jf*

/

d4' I-ll,

[

1.

t*)R,£i,-*"htm I3.'l I*.

j.&o*

Ii 4~-~LLLiI Atgelf7>2 bJY+ Y ant is used, sample must be diluted..Reviewed by: lZ l.* IIII Date reviewed

" j SOP C7 -Exhibit C7. l, revision 06-29-09

U7 Sequoyah Nuclear Plant Biomonitoring November 17 - 24, 2009 Appendix D Reference Toxicant Test and Control Chart

-Page 78 of 102

I Pimephales promelas Chronic Reference Toxicant Control Chart.

D Organism Source: Aquatox, Inc,.

Environmental Testing Solutions, Ic.

1.0 USEPA Control Limits (+ 2 Standard Deviations)

]

0.6 I 0.4 I-19, L J*

Q Q

N 1.0 0.8

.USEPA Warning and Control Limits (75t2and 9(/.Percentile CVs)--

I I

t I

I I

t I

t t

i t

I t

I I

t t

0.6 0.4 1.2 1.0 0.8 0.6 0.4 I

I I

J I

I 1

I I1 I

I I

I Laboratory Warning and Control Limits(10J and 25 Percentile CVs)

L l

Test date 7-day IC 25 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC 2 )

. Warning Limits (mean IC 25 +/- S A.0 or SA.75)

.......... P.PgCntr 7

9 Limits (mean IC 25 +/- SA.25, SA.907. or 2 Standard Deviations)

ETS Environmental Testing Solutions, Inc.

Test number Test date 7-day IC25 CT (g/L KCI)

(g/L KCI)

Pimephales promelas Chronic'Reference Toxicant Control Chart State and USEPA Lalboratory Laboratory USEPA S

Control Limits.

S, Warning Limits S&25 Control Limits SA75 Warning Limits SAM CT -2S CT + 2S CT-S,.10 CT+St, 1

CT-S-25 CT+SC.2I CT - S,.7.

CT + SAMs USEPA Control Umits CT-Sk CT'+ S,"

Cv I

2 3

4 5

6 7

8 9

10 II 12 13 14 15 16 17 18 19 20 02-25-09 02-27-09 03-03-09 03-10-09 03-10-09 04-14-09 04-21-09 05-05-09 05-12-09 06-09-09 06-18-09 07-07-09 08-04-09 08-11-09 09-15-09 10-06-09 10-14-09 10-28-09 11-03-09 11-17-09 0.76 0.72 0.75

.0.79 0.77 0370 0.80 0.69 0.75 0.76 0.78 0.72 0.78 0.81 0.72 0.78 0.77 0.73 0.67 0.79 0.74

.0.74 0.75 0.76 0.75 0.76 0.75

.0.75 0.75 0.75 0.75 0.75 0.75 0.75 0.75 0.76 0.75 0.75 0.75 0.03 0:68 0.80 0.09 0.65 0.02 0.70 0.79 0.09 0.65 0.03 0.69 0.81 0.09 0.66 0.03 0.70 0.81 '

0.09 0.67 0.03

.0.68 0.82 0.09 0.66 0.04 0.68 0.83 0.09 0.66 0.04 0.67 0.83 0.09 0.66 0.04 0.67 0.82 0.09 0.66 0.04 0.68 0.82 0.09 0.66

.0.04 0.68 0.82 0.09 0.66 0.04 0.68 0.82 0.09 0.66 0.04 0.68 0.82 0.09 0.66 0.04

,0.68 0.83 0.09 0.66 0.04 0.68 0.83 0.09 0.66 0.04 0.68:

0.83 0.09 0.66 0.04 0.68 0.83 0.09 0.66 0.03 0.68 0.82 0.09 0.66 0.04 0.67 0.83 0.09 0.66 004 0.67 0.83 0.09 0.66 0.83 0.83 0.85 0.85 0.84 0.85 0.84.

0.84 0.84 0.84 0.84 0.84ý 0.85 0.84 0.84 0.85 0.84 0.84 0.84 0.15 0.58 0.89 0.16.

0.59 0.90 0.16 0.60 0.91 0.16 0.60 0.92 0.16 0.59 0.91 0.16 0.60 0.91 0.16 0.59 0.90 0.16 0.59 0.90 0.16 0.59 0.91 0.16 0.59 0.91 0.16 0.59 0.91 0.16 0.59 0.91 0.16 0.60 0.91 0.16 0.59 0.91 0.16 0.60 0.91 0.16, 0.60 0.91 0.16 0.60 0.91 0.16 0.59 0.91 0.16 0.59 0.91 0.28 0.28 0.29 0.29 0.28 0.29 0.28 0.28 0.28 0.29 0.28 0.29 0.29 0.29 0.29 0.29 0.29 0.28 0.29 0.46 0.46 0.47 0.47 0.46 0.47.

0.46 0.46 0.46 0.47 0.46 0.47 0.47 0.47 0.47 0.47 "0.47, 0.46 0.47 1.02 0.33 041

.1.02 0.33 0.41 1.04 0.34 0.41 1.05 0.34 0.42 1.03 0.34 0.41 1.04 0.34 0.42 1.03 0.34 0,41 1.03 0.34 0.41 1.03 0.34 0.41 1.04 0.34 0.41 1.03, 0.34 0.41 1.04 0.34 0.41 1.04 0.34 0.42 1.04 0.34 0.41 1.04 0.34 0.41 1.04, 0.34 0.42 1.04ý 0-34 0.41 1.03 0.34 0.41 1.04 0.34 0.41 1.07 0.04 1.08 0.03 1.09 0.04 1.10 0.04 1.09 005 1.10 0.05 1.08 0.05 1.08 0.05 1.09 0.05 1.09 0.05 1.08 0.05 1.09 0.05 1.09 0.05 1.09 0.05 1.09 0.05 1.10 0.05 1.09 0.05 1.09 0.05 1.09 0.05 Note:

7-d IC, = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

CT = Central tendency (mean ICs).

S = Standard deviation of the IC25 values.

Laboratory Control and Warning Limits

-Laboratory control and warning limits were established using the standard deviation of the lCas values corresponding to the 10th and 25th percentile CVs.

These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

SA.IG

= Standard deviation corresponding to the 10'h percentile CV. (SA:O-I 0.12)

S,.

- Standard deviation corresponding to the 25'h perccntile CV. (SA,2S = 0.2 1)

USEPA Control and Warning Limits

= Standard deviation corresponding to the 7 5 "a

percentile CV. (

5 75 = 0.38)

Sg.j= Standard deviation corresponding to the 90 percetile CV. (Sg = 0.45)

CV = Coefficient of variation of the IC2s values.

i USEPA. 2c0D. tlNnd mSidi

.aW Aewuo59 fbr MehdW VauiadY o WhOle Emoanf Tonmy Appbli,.Ms Uodff the Naiiroal Pollutam Dischage Eblonjaia Proprarn. EPA-t33-R-00-003. US E-m armiat oftdima Agaeny, Cnnria. OR-Organu.s obsamdfrom Aquat". Ine.

atoxl 1-17-09

0 Precision of Endpoint Measurements D ETS Pimephales promelas 3F

ý

Chronic Reference Toxicant Data Environmental Testing Solutions, Inc.

Control Control Mean Test number Test date..

Survival Growth.

CT CV CT

-MSD PMSD CT for Conirol Growth

(%)

(mg/larvae)

(mg/larvae) for Control

(%)

Growth CV (%)

(%)

for PMSD (%)

2 3

4 5

6 7

8 9

10 11.

12 13 14 15 16 17 18 19 20 02-25-09 02-27-09 03-03-09 03-10-09 10-09 04-14-09 04-21-09 05-05-09 05-12-09 06-09-09 06-18-09 07-07-09 08-04-09 08-11-09 09-15-09 10-06-09 10-14-09

.10-28-09 11-03-09

'11-17-09 1oo 100 100 100 100 100 100 100 100 100 100 100 100 1oo 100 100 97.5 100 100 97.5 0.659 0.578 0.664 0.661 0.696 0.671 0.642 0:805 0.717 0.703 0.790 0.763 0.692 0.583 0.723 0.894 0.758 0.855 0.757 0.825 0.618 0.634 0.640 0.652 0.655 0.653 0.672 0.677 0.679 0.689 0.696 0.695 0.687 0.690 0.702 0.706 06714 0.716

.0.722 8.2 6.8 8.1 6.8 8.8 10.3 8.3 7.9" 7.0 2.5 6.7 5.7 6.2 8.0 9.6 12.9 7.6 6.5 7.1 8.3 7.5 7.7 7.5 7.7 8.2 8.2 8.2 8.0 7.5 7.4 7.3 7.2 7.2 7.4 7.7 7.7

.7.7 7.6 7.7 0.07 0.08 0.08 0.09 0.09 0.08 0.07 0.07 0.06 0.08 0.09 0.1 1 0.07 0.05 0.08 0.12 0.14 0.10-0.07 0.10 11.1 13.1 12.3 12.9 12.6 11.4 11.2 8.4 8.9 10.7 11.7 14.2 10.7 9.3 10.4 13.2 18.3 12.0 8.8 11.8 12.1 12.2 12.4 12.4 12.3 12.1 11.6

.11.3 11.3 11.3 11.5 11.5 11.3 11.3 11.4 11.8 11.8 11,6 11.7 Note:

CV Coefficient of variation for control growth.

Lower CV bound determined by USEPA (10th percentile) 3.5%.

Upper CV bound determined by USEPA (90th percentile) - 20%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10' percentile) = 12%.

Upper PMSD bound determined by USEPA (90"' percentile) = 30%.

CT = Central Tendancy (mean Control Growth, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole EffluentToxicity Test Methods, Volumes I and 2 Appendix. EPA-82 I-B-01-004 and EPA-82 I-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

Organisms obtainedfrom Aquatox, Inc.

Page 81 of 102 atoxl 1-17-09

0"ET

.:,'l Environmental-Testing Solutions, Inc.

Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.

I.Lfl

.4-1.00 0.75 0.50 0.25 30

.20 o

10 o

0

' ~ -

I I

USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)

Kentucky Acceptance Limit (< 30.0%)

i I

I I

I*

I I.

I I

USEPA Upper PMSD Bound (9 0d1 percentile < 30.0%)

a rj~

30 20 10

-1 I

~

i*~l

~

I

~

I I

I I.

I I~*

I I

I 1

I I -

i 1

I Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD)

... Pagv* t kllInmits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Ii S

S EnTS,nT.

g Page I of 5 Ii I

Potassium Chloride Chronic Reference Toxicant Test (EPA.-821-R-02-013 Method 1000.0)

Species: Pimephales promelas pKCICR Test Number:

I__

Dilution preparation information:

Comments:

KC] Stock INSS number:

I t.)

" 1 "41._'_*_-_

Stock preparation:

50 g KCL/L:

Dissolve 50 g KCI in 1-L Milli-Q water.

Dilution prep (rg/L) 450' 600 750 900 1050 Stck volume (mL) 9

.12 15 18 21 Diluenitvolume(mLF 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:

Test information:

Organism age:

NS Irkoju.Z 0%-b Randomizing template:

?i A-..

Date and times organisms

-\\\\-*-a-*

Oc Incubator number and were born between:

shelf location:

Organism source:.

Artemia CHM number:

t'* LI4LI Drying information for weight

__determination:

Transfer vessel pH =

S.U. Temperature =

0C Date / Time in oven:

l r'rA-o* %%%(b information:

11". (0 Initial oVen temperature*

too..

Average transfer volume:

Date / Time out of oven:

IL-181411 IlqO

0.

Final oven temperature:

t.* C Total dyinzg time:

Daily feeding and renewal information:

i.i Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used.

Time Analyst Time Analyst Time.

Analyst 0

i'~ioj

\\t~

LtO 0~t' I-__

q

~

a

-0e AL IVA_____

o2

-t x 00 a o 1"40

%TzVL.0 4,

V.o 7\\-K I&

I 1-.10-601 Od 00 Ok&

Iq.b wtz'.lq

%L*

act'-6 b

v---oi lqoa AL 1210*

'~o 5 l-a-oi O.&oG I4

%AO.v 6

L %-L%-01t h,i,

t bo Id

% -LZz,

-2 r, I.t 7

ý 1%-2+01 "ti Control information:

Acceptance criteria Summary of test endpoints:

% Mortality:

7, "

20%

7-da, LCso 91 Ri(44 Average weight per. initial larvae:

I.8 NOEC j

(000 Average weight per surviving larvae:

O.gqS 1 0.25 mg/larvae' LOEC0 ChV

(.10.9 IC25 1 -797-3 Page 83 of 102 SOP AT21 - Exhibit AT2 1.1, revision 04-01-09

0 0

S9 J

En*on.,,.,,la T*. nq Sols, 0.0.

Page 2 of 5 A

PpKCICR Test Number:

195.

Species:' Pimephales DroMelas "I

Survival and Growth Data Day Control 450 me KCV/L 600 mg KCVL

.0_.____ "

A B

C D

E F

G H

I J

K L

to

/0

'0

/0

/t

/0

/(

/0 1t (0

/0

/0 t0

/0 t6 (0

/0

/0 t)

/0 to 10 10

/0 10 "10 10.10 10 I0

.10 3

I o

/!

/0.

10

/03

/O

/03

/0

/!0

/0 4(I0 tO I U

/0 O

1/0

/

(0

&O I0 4

(1 10 10

,' 1k to 10:)

10

10) t O1(' to 5i

6.

10

.O10

~.

10, 10

1. 0

/1 10 10 10 I______

/3, 1I C)

~

10 10 V4

'c0 10

'V0

-o at

'VA-I-iIo t

1 S

A Pan weight (mg)

Tray color code:i "Y410.)

Analyst:

(..

l> I Sto IS.7, l' -

j P 1i,

7. 4 I.,

I.-I

/q.s o (4.),* i'.I.1 Date:,

-"-Z2,- "-

B = Pan + Larvae weight (mg)

Analyst: %

e-Z,*

4

.1 7,.7 Z.*

Z-.02-Z.

7.

-Z.*

T-4 2.0' 7, 0 Z 6 -*\\Z \\ Zý.

Date: IL_.t01.

Z2 o-C = Larvae weight (mg)

= B - A.

6.

' e 5

A Weight per initial number of larvae (mg)

- C / Initial number of larvae A.

5b 0,

~*

AI, 01 N

0*

I Average Percent weight per reduction initial from control 0 SVI.5 number of

(%)

larvae (_E)

-I R3 I

S. X7.-~

Comment codes; c = clear, d = dead, fg= fungus, k = killed, m = missing, sk sick, sm = unusually small, Ig ý unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

C.

Page 84 of 102 SOP AT21 - Exhibit AT2 1. 1, revision 04-01-09

F 3

DTS Page 3 of 5 PpKCICR Test Number:

1 Species: Pimephales promelas Survival and Growth Data _

Day 750 me KCI/L 900 me KCVL 1050 m KCVL SI___

M N

0 R

S T

U V

W

ýX 0 1 (3 J ()

1o to

, o to '1o 1o 00 10 M

Ot I 0

/l0 to/,

10 1

oak SA

q Ut wk q

4 2

o-1 0

5

-1 s

70 01 S1 C)

(

5.

S 4

5--

_~~

1 A~

.7 g

4 i

R A = Pan weight (mg)

T ray color code:: )A 4O &

K lq-.'4b 14FI3 Analyst: -4A.,t l'f,,Dy 13, 4...

l5..? Zl",L* 7-,

)

I3 Date: IO"L 'O.,

B = Pan t Larvae weight Analyst:

VA.

Z O.2 c z2-M3 tO.

Al-eq.5q iI.ýai'.

i*.73 I(L.q2 ')s Ii,.'(

Date:

i "Z.01 -01)'

C = Larvae weight (mg)

,= B-A -

&.q t

SR 4t 11,

q. S S L.O S. &b

'ZO 3 &A IS S 1,ta 1.-.63 1.', 1 Weight per initial number of larvae (mg)

X%'O SC / Initial number of larvae N".*

)

Average Percent.

weight per reduction

-61 0.

initial from control O:'S

'a.'.-

number of

(%)

larvae (mg)

.f""

Comment codes: c= clear, d dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

Page 85 of 102 SOP AT21 - Exhibit AT2 1. 1, revision 04-01-09

Environmental Testing Solutions, Inc.

Pimephales promelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Teat number.

PpKOCI #193 Teat dates:

November 17-24, 2009 Reveiwed by:

Coaceantonlmo (maL Replkale d1aiaumbetra Fotlnumbered A Pm. eighl B=Pu+L.a.

Larvame wght (mg)

WewSnghs inS g Mew elgah/

Coefflde.5 varlal.

Weldghsllciddasber Mean narviv,.1 leC *m.hld al "ele

~

kt d

Pertcat red*t*d.ewu KCI) la e

lar-e (ow,

wefght (.W A -AB unite-dlarvae(mue S.. riving ollmb or, r oi,.

tw e

ofhtume- (n) 1W%)

eaabe-6larvae vuariatio Y) control (1/)

larme (mg)

,m*b*,,

(t)

(.ie A

10 to 13.90 22.97 9.07 0.907 0.907 B

to 10 15.09 23.14 8.05 0.805 0.805 Cotrol C

I 0 1 0 13.58 22.01 8.43 0843 0.845 5.2 043 9

D 10 9

13.21-20.65 7.44 0.827 0.744 E

10 9

14.99 22.02 7.03 0.781 0.703 430 F

10 10 13.74

21.33 7.59 0.759 07803 5.2 5997.5 0.783 A

5.1 G

10 10 12.68 21.22 8.54 0.854 0.854 H

10 10 12.51 20.67

.8.16 0.816 0.816 1

10 9

14.11 21.90 7.79 0.865 0.779 60J 10 10 14.50 22.98 8.48 0.848 0.948 93 6K 10 10 14.06 21.21 7.15 0.715 0.826

19.

0.715 98.0

,.783 7.0 I

L 10 9

14.34 22.23 7.89 0.877 0.789 M

10 8

13.48 20.32 6.84 0.855 0.684 N

10 7

14.64 20.95 6.31 0.901 0.899 4.6 0.8391 77.5 "98 15.9 15-3 750 0

10 9

13.84 22.43 8.59 0.954

_ P I0 7

14.21 20.40 6.19 0.884 0.619 Q

0 4

14.79 19.12

. 4.33

'1.083 0.433 R

10 5

15.29 19.54 4:25 0850 0.892 14.4 0.425 42.5 0.378 15.7 54.2 S

10 4

13.62 16.82 3.20 0.800 0.320 T 1 10 4

14.31 17.65 3.34 0.835 0.334 U

10 2

15.18 16.73 1.55 0.775 0.155 V

10 I

15.72 16.90 1.18 1.180 0.894 218 0.118 17.5 0.149 15.0 81.9 100 W

10 2

14.42 15.95 1.53 0.765 0.153 X

10 2

14.73 16.44 1.71 0.855 1

0.171 Dunoett's MSD value:

PMSD-0.097s 11.8 MSD =

Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimut percent difference between the control and treatment that can be declared statistically significant in a whole effiueit toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratmy Variability Study (USEPA, 2001 a; USEPA, 2001 b).

USEPA 2001a, 2001b. Final Report: lnterlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-0OI-005. US Environmental Protection Agency, Cincinnati, OH.

"ETS SEnvtronental TetingS Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:

11/17/2009 Test ID:

PpKCICR Sample ID:

REF-RefToxicant End Date:

11124/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

KCL-Potassium chloride Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

Cooc-mg/L 1

2 3

4 D-Control 1.0000 1:0000 1.0000 0.9000 450 0.9000 1.0000 1.0000" 1.0000 600 0.9000 1.0000 1.0000 0.9000 750 0.8000 0.7000 0.900 0.7000 900 0.4000

0.5000 0.4000 0.4000 1050 0.2000 0.1000 02000 0.2000 Transformu Arcsin Square Root I-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Resp Number D-Cohtrol 0.9750

'1.0000 1.3713 1.2490 1.4120 5.942 4

1" 40 450 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4

0.000 2.410 0.1471 1

40 600 0.9500 0.9744 1.3305 1.2490 1.4120 7.072 4

0.668 2.410 0.1471 2

40

750 0.7750

.0.7949 1.0846 0.9912 1.2490 11.294 4

4.696.

2.410 0.1471

'9 40

900 0.4250 0.4359 0.7099 0.6847 0.7854 7.091

4.

10.836 2.410 0.1471 23 40

'1050 0.1750 0.1795 0.4282 0.3218 0.4636 16.570 4

15.451 2.410 0.1471 33 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.91163939 0.884

-0.1384496t -0.6290117 Bartlett's Test indicates equal variances (p = 0.82) 2.20082235 15.0862722 Hypothesis Test (l-tail, 0:05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df

.Dwmetfs Test 600 750 670.820393 0.07612662 0.07923945 0.63093674 0.00745088 7.3E-12 5,18 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slone 1190075 202798845 7.9258926 15.8756075 0.025 0.02529665 7.81472778 I

2.94271355 0.08402832 3

Intercept TSCR Point ECOI EC05 ECI0 ECI5 EC20 EC25 EC40 ECSO EC60 EC75 EC80 EC85 EC90 EC95 EC99

-30.020498 0.02489373 Probits 2.674 3.355 3:718 3.964 4.158 4.326 4.747 5.000 5.253 5.674 5.842 6.036 6.282 6.645 7.326 5.96965262

-41.721018

-18.319979 0.0167698

-0.0079751 0.05776254 mg/L 95% Fiducial Limits 558.771764

.439.30398 632.540335 637.528005 533.944092 700.273975 683.954056 591.864566 740.053467 717.172783 633.972402 768.743169 744.720889 669.099597 792.886858 769.196521 700.275338 814.793373 834.497847 781.392728 877.222284 876.422566 829.69787 922.532758 920.453569 875.571987 976.186221 998.595923 946.148753 1085.24501 1031.4153 973.075482 1134.89329 1071.03413 1004.29166 1196.99431 1123.05276 1043.78666 1281.46378 1204.83569 1103.60002 1419.80285 1374.65171 1222.28693 1724.95223 Organisms obtained from Aquatox, Inc.

atoxlI 1-17-09

I,........,

Environmental Testing Solutlons, Inc.

Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:

11/17/2009 Test ID:

PpKCICR Sample ID:

REF-Ref Toxicant End Date:

11/24/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

KCL-Potassium chloride Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

Conc-mg/L 1

2 3

4 D-Control 0.9070 0.8050 0.8430 0.7440 450 0.7030 0.7590 0,8540 0.8160, 600 0.7790 0.8480 0.7150 0.7890.

750 0.6840 0.6310 0,8590 0.6190 900 0.4330 0.4250 0.3200 0.3340 1050 0.1550 0.1180 0,1530 0.1710" Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV%

N, t-Stat Critical MSD Mean N-Mean D-Control 0.8248 1.0000 0.8248 0.7440 0.9070 8.285 4

0.8248 1:0000 450 0.7830 0.9494 0.7830

,0.7030.

0.8540 8.441 4

0.933 2.180 0.0975 0.7830 0.9494 600 0.7828 0.9491 0,7828 0.7150 0.8480 6.959 4

0.939 2.180 0.0975 0.7828 0.9491 750 0.6983 0.8466 0.6983 0.6190

'0.8590 15.872 4

.0.6983 0.8466 900 0.3780 0.4583 0.3780 0.3200 0.4330 15.676 4

0.3780 0.4583 1050 0.1493 0.1810 0.1493 0.1180 0.1710 14.966 4

0.1493

, 0.1810 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93505901 0.805

-0.079593823

-1.15766298 Bartlett's Test indicates equal variances (p = 0.93) 0.14925823 9.2103405 Hypothesis Test (1-tall, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600

>600 0.09751355 0.11823407 0.002338083 0.00400172 0.57733494 2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL(Exp)

Skew IC05*

444.48 171.45 43.33

.936:54

-0.1280 ICI0 671.85 93.51 216.91 825.11

-1.2941 IC15 745.05 48.64 537.03 808.42

-0.9607 IC20 768.01 29.70 646.35

,819.59

-1.0239 IC25 787.32 20.62 712.96 835.30

-0.5410 IC40 845.27 13.72 797.28 882.88

-0.0132 IC50 883.90 13.24 846.50 930.45 0.3616

indicates IC estimate less than the lowest concentration Organisms obtainedftom Aquatox, Inc.

atoxil1-17-09

I EnVImnanmUIT..iIn, Sokntto.,j Inc.

Page 4 of 5 Species: Pimephales Dromelas PpKCICR Test Number: ____

Daily Chemistry:

Day Concentration Pa'rame'ter PH (S.U.)

-4.qe I 74 7 Ia

'7.SQ C.q DO (mg/L)

Conductivity (A mho/cm) 3 S35.328 CONTROL Alkalinity (mg CaCO3/L)

A Hardness (mg CaCO3 L)

Temperature pHi (s.u.)

r'l 450 mg KCIL Conductivity 450AmgKCI/L' 110 "1*

luO I[00 Temperature

-LA.

(c)nd 600 mg KCI/L Conductivity y

(Amhos/cm)

~

S(

Temperature DO (s.)

I.4

.750 mg KCI/L Conductivity

.O 1090 1o Temperature

(-c) pH (S.U.)

IIjs1 ;,510 1 '41m' HI.-I.q I

-1.1C, I4 I g

F II DO (m")

900 mg KCVL Conductivity (ji.mhos/cm)

"'4 7.3 11.8 1

4.

14 U-'L LA.W Temperature (0C) pH (S.U.)

I f4iýp I 'I.I.Q q 7-44 1 'mot.

-11 Q.4-7,0 - I DO (mgf/L)

I-1050 mg KC/L Conductivity (umhos/cm) 19 0 00(i 14-8 OL4.e

.2q. j..,. -zs.

z4-4 zq.,f Temperature

(°C,)

STOCK rConductivity G O0 I (Rdhslm Initial I

SOP AT21 - Exhibit AT2 1.1, revis Page 89 of 102

S EA~fr~.n,.nt.IT.u1ng Page 5 of-5 Species: Pimephales promelas PpKCICR Test Number: J I

Dayv I

Do I

Analyster Concentra'tio~n aamt

~-

t..~

I pH (S.U.)

I DO (mJ/L)

Conductivity (ramhos/cm)

CONTROL Alkalinity (me CaCO /L)

JM7

-I.GS

-4.4a "U

Ile

-334 ILA.

-LA.1 ILA k vo, vk Hardness *

(mg CaCO3/L)

Temperature (0C)

I I[

I-I III pH (S.U.)

/ý-80 II -7.5q 1

UA~ 11 13 11 1~ q,4 1z '110o 1

450 mg KCI/L Conductivity (Tmhospcm)

Temperature (0c) 0-L #1~

.6 7-L PH (S.U.)

7,1 1UO

".12

,I

l-600 mg KCVL Conductivity q

(Rtmho /M)

Temperature

" 1.

l

-4.

VA k e

(0c)

~ mgKCVL Conductivity 75 0 mg KCI/L inhos/cm0 l.I,£* O 1 00l q

Temperature

-D.'4 Io.

ILA. rII

.r PH (S.U )

7.Le 1 -1

.LP

"-., IP

, -3

':.'3 -

1*e00

.~

1050 mg KCI/L Conductivity I

(jimhos/cm) 21(O2C)

(Q Temperature vk.l (0c),

-L,.

-(.U1-76

--2q

-4

-)

-4 Initial Final Initial Final Initial Final Initial Final

SOP AT21 - Exhibit AT2 1.1, revision 04-01-09 Page 90 of.102

Environmental Testing Solutions, Inc.

Ceriodaphnia dubia Chronic Reference Toxicant Control Chart 1.12 1.08 1.06 1.04 1.02 2.5 I.. "

I I

l" I

I USEPA Control Limits (+2 Standard Deviations)

I I

Q Jn IU 2.0 1.5 1.0 0.5 I

I I "

I I

l I

I.

I l

I I

, I USEPA Warning and Control Limits (75 th and 9 0th Percentile CVs)

+

.I I

I I

1.4 1.3 1.2 1.1 1.0 0.9 0OR I

I '

I I

I i

! I I

I I

I I I

I I

I Laboratory Warning and Control Limits (10 th and 251h Percentile CVs)

I..................

I****-------------

- -o 08v Test date 7-day IC2s = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

Central Tendency (mean lC 25)

Warning Limits (mean IC25 SA.10 or SA.75)

....... aCet iits (mean 1C25 +

SA.25' SA.90, or 2 Standard Deviations)

1ETS Environmental Testing Solutions, lncý Test number Test date 7-day JC' CT (g/L NaCI)

(g/L NaCI)

Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEPA S,

Control Limits SA."

CT-2S CT+2S Laboratory Warning Limits CT - SA 0o. CT + SA0o Laboratory-SAns Control Limits CT - SA.25 CT + SAm USEPA".

SL75 Warning Limits.

,S CT - SA.75 CFt Sý5 USEPA Control Limits CT - Sk.o CT + SA" 1

2 3

4 5.

6 7

8 9

10 S11 12 13 14 is 16 17 18 19 20 11-04-08 11-04-08 12-02-08 01-06-09 02-03-09 02-24-09 03-10-09 04-14-09 05-05-09 05-05-09 06-09-09 06-09-09 06-18-09.

07-07-09 08-04-09 08-04-09 09-15-09 10-06-09 10-14-09 11-10-09 1.07 1.10 1.06 1:05 1.05 1.07 1.07 1.09 1.07 1.08 1.07 1.07 1.06 1.06 1.07 1.09 1.05 1.08 1.07 1.07 1.08

0.02 1.05 1.12 0.09 1.00 1.08 0.02 1.04 I.iI 0.09 0.99 1.07 0.02 1.03 1.I1 0.09 098 1.07

-0.02 1.03

.1.10 0.09 0.98

.1-07 0.02 1.03 1.10 0.09 0.98 1.07 0.02 1.04 1.10 0.09 0.98 1.07 0.02 1.04 1.10 0.09.

0.98 1.07 0.02 1.04 1.10 0.09 0.98 1'07 0.02 1.04 1.10

'0.09 0.99 1.07 0.01-1.04 1.10 0.09 0.99 L.07 0.01 1.04 1.10 0.09 0.99' 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.04

.10 0.09 0.98 1.07 0.01 1.05 1.09 0.09 0.98 1.07 0.01 1.05 1.10 0.09 0.99 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.04 1.10 0.09 0.99 1.07 0.01 1.05 1.10 0.09 0.99 1.07 0.01 1.05 1.10 0.09 0.99 1.17 1.16 1.16 1.15.

1.15 1.15 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 1.16 0.18 0.90 0.18 0.89 0.18 0.89 0.18 0.88 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89 0.18 0.89, 0.18

-0.89 0.18 0.89 0.18' 0.89 0.18 0.89 0.18 0.89 0.18 0.89 1.27 1.26 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 0.49 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.60 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 1 571 0.67 0.41 156 0.67 0.41 1.55 0.66 0.41 1,55 0.66 0.41

155, 0.66 041 155 066 0.41 155-0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55;'"

0.66 0.41 155 0.66 0.41 155*:ý 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1L55-'

0.66 0.41 1.55 0.66 0,41 1.55 0.66 041 1.75 1.74 1.73 1.73 1.73 1.73 1.73 1.73 1.73 1.74 1.74 1.73 1.73 1.73 1.74 1.73 1.73 1.73 1.74 Cv, 0.02 0.02

0.02 0.02 0.02 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 Note:

7-d IC25 day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

CT - Central tendency (mean IC25).

S - Standard deviation of the IC-5 values.

Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation ofrthe ICs values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

S.1o = Standard deviation corresponding to the l0i percentile CV. (Skio = 008)

Sas = Standard deviation corresponding to the 2P percentile CV. (SAZs = 0.17)

USEPA Control and Warning Uimits S'Is = Standard deviation corresponding to the 75P percentile CV. (SA~s = 0.45)

SA..0 = Standard deviation corresponding to the 90eh percentile CV. (SAgo = 0.62)

CV Coefficient of variation of the IC2s values.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

I-Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data

" Test.

Tes da

-Control, Control Mean'.

uestr ate.

Rep.routn

,-CT number,............

Survival.... 0.Keproducuion..

Cv CT.

-' MSD PMSD CT

(%)

(offspring/female)

.1 2

3 4

5 6

-7 8

9 10 11 12 13 14 15 16 17 18 19 20 11-04-08 11-04-08 12-02-08 01-06-09 02-03-09 02-24-09 03-10-09 04-14-09 05-05-09 05-05-09 06-09-09 06-09-09 06-18-09 07-07-09 08-04-09 08-04-09 09-15-09 10-06-09 10-14-09 11-10-09 100 100 100 100 100 100 100 100 100.

100 100 100 100 100 100 100 100 100 100 100 32.3 31.5 31.5 33.2 31.4 32.2 31.9 33.9, 33.6 34.6 31.3 31.1 32.3 29.9 30.4 32.0 31.6 31.4 33.3 34.0 for Control Mean Reproduction (offspring/female) 31.9 31.8 32.1 32.0 32.0 32.0

'32.2 32.4 32.6 32.5 32.4 32.4 32.2 32.1 32.1 32.0 32.0 32.1 32.2 for Control

(%)

Reproduction CV (%)

(%)

for PMSD (%)

5.1 6.2 4.6 4.0 6.6 6.0 5.8 6.7 4.3 7.7 6.4 9.4 7.3 3.3 4.7 5.1 5.4 4.3 3.5 6.2 5.6 5.3 4.9 5.3 5.4 5.5 5.6 5.5 5.7 5.8 6.1 6.2

.6.0.

5.9 5.8 5.8 5.7 5.6 5.6 2.2 2.4 2.5 2.0 1.9 1.8 2.6 2.0 2.3 2.2.

2.1 2.6 1.9 2.2 2.2 2.2 2.2 2.4 2.6 2.6 6.8 7.7 8.0 6.1 6.0 5.7 8.2' 5.8 6.8 6.5 6.7 8.3 5.8 7.2 7.3 6.9 7.0 7.6 7.7 7.7 7.2 7.5 7.1 6.9 6.7 6.9 6.8 6.8 6.8 6.7 6.9 6.8 6.8

'6.9 6.9

.6.9 6.9 7.0 7.0 Note:

CV = Coefficient of variation for control reproduction.

Lower CV bound determined by USEPA (10th percentile) = 8.9%.

Upper CV bound determined by-USEPA (90t" percentile) =42%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the:control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (I0th percentile) = 13%.

Upper PMSD bound determined by USEPA (90th percentile) = 47%.

CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 200lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01l-005. US Environmental Protection Agency, Cincinnati, OH..

g 93 of 1-10-09.

Page 93 of 102.

0"ETS

,*]'i.Environmental Testing Solutlorns, Inc.

Ceriodaphnia dubia Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 40 35

-C S20 I '-. 1

9 15

.I I

I I

I I

I

.1 I

I I

I I*

I I

.......... 4....... 0..............................

Minimum Acceptance Criteria (> 15.0 offspring per surviving female)

I I

I II I

g.40 4f,I 20 10 "30 420 5.0 40 L

I I

I

. I I

I I

I North Carolina Acceptance Limit (< 40.0%)

Kentucky Acceptance Limit (<30.0%)

I I

-I I

I I

.I I.I....I...

r0) 30 20 10 A3 I

I I

USEPA Upper PMSD Bound (90 th percentile < 47.0%)

S.

v Test date Control Reproduction, Coefficient of Variation (CV),.or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

-"Central Tendency (mean Control Reproduction, CV, or PMSD) pa4l* */Anits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Ir l:T

':E T Sl'la

~t m dtLl¢ Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia CdNaCICR #:

I-00 Dilution preparation information:

Comments:

NaCI Stock INSS number:

l r4*,

Stock preparation:

100 g NaCI/L:

Dissolve 50 g NaCI in 500 mLMilli-Q water.

D~lution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9

" 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 1

Test organism source information:

Test information:

Organism e:

I< 24-hours old Randomizing template color:

Dateand times organisms were born i,-"b-O% 0l.W 'r IDO Incubator number and shelf between:

location:

2.6 7-.-

cultur e board:

Replicate number: 1 2

3 4 1 6

7 9

9 10 YWT batch:

Culture boardcup number: 1%

k I

i 1

W_1&

1.

-&EI v1*Of Transfer vessel information:

pH = _'.

S.U.

Temperature = 1.

°C Selenastrum batch:

I l-0I oi Average transfer volume (mL):

0. 0 51s Daily renewal information:

Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0i 0

I-1 1otto I t-01-- 01 A

2)G4a 2

IlV-1o' l-01-' A 24 102*OA lo-tst-A 5

ii-r-o' i J

Control information:

Acceptance criteria Summary of test endpoints:

%_ofMaleAdults:

s 20%

7-day LC50 b U

% Adults having 31d Broods:

I a 80%

NOEC

&t

% Mortality:

O7.

s_ 200/6 LOEC i to Mean Offspring/Female:

34 o>

15.0 offspring/female ChV 0/. CV:

-7.1

<40.0%

l.C_

10-12L.,

I Page 95 of 102 SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09

I FT*

Species:- Ceriodaphnia dubia CONTROL Page 2 of 6 aCICR U:100 Survival and Reproduction Data q

Replicate number Day 1

2 3

4 5

6 7

8 9

10 Young produced Q

I I)

hh I

Adult mortality t.-

L

._.s

\\_

.2

!. Young produced C)

C)

(

)

(

Adult-mortality t-t 3

Young produced 0 Q p

0 P

0 C

I" C

Adult mortality t *

\\

1-....

4 "Young produced l

Adult mortality i

\\.

L. _

5 Young produced V5, to I4 5L I

[L Adult mortality 6

.Young produced 0

j 0

A It l

IL 0

V--.

Adult mortality

[ %

7 Young produced I

%to I

Ile I

Total young produced

56.

3%

3\\.

35

36.

'3:S 3**3\\

Final Adult Mortality W

W

?

Xfor 3' Broods "7-1 5

-1 Note: Adult mortality (L = live, D dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

. % Mortality:

C o7.

Mean Offspring/Female:

Z4'. 0 600 -mg NaCI/L Survival and Reproduction Data Replicate number Day 2

3 "4

5

-6 7

8.

9 10 1I Young produced C)

C I"Adult mortality 7---

2 Young produced 0C C

Adult mortality 1 1L.

3 Young produced (5-7 o

)

"...Q0 0

-c

)

Adult mortality i_

L_

L L

L.._

4 3 Young produced 0=

Adult mortality 5

Young produced

\\

.4i

ŽL.

S S

I Adult mortality

\\..

t.

I..

L...

L 6

Young produced 75 0

(3 (3) 0 Adult mortality t__

, [

L L.%

L.L-I L

L 7

Young produced 14ý Ile l

V)

.1 U

Total youngproduced I s 3

j,*

3S 30 Final Adult Mortality..

L T

t.

C.

T?

K..

Note: Adult mortality (L = live, D dead), SB = split brood (single brood split between two days), CO = carry over (offspring earried over with adult duirine tranqf'er*

A-:*

g,'i carried over with adult durina transfer)

Concentration:

% Mortality:

07.

Mean Offspring/Femate:

-*. 0

% Reduction from Control:

0 7.

SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09 Page 96 of 102

,*S Page 3 of 6 Species: Ceriodaphnia dubia

..800 mg NaCI/L,

CdNaCICR #:- tO0. -

Survival and ReDroduction Data Replicate number

-Day 1.2 3

4 5

6 7

8 9

10 YVoungproduced

.C

-*0.

C C'(I 0

n 0

Adult mortality

.Young produced.,.:

r)

)

C5ft

)

C) 0-7 Adult' mortality -

C t,

3 Young produced

-CL

[

C C

O

Ž C)

Adult mortality L.1 4

Young produced S

.4 Adult mortality L-t s

Young produced Oil-L 10 1 IL.

I It. L

'L I--

Adult mortality

\\

\\. -

\\-

v L

\\--

6 Young produced

'=

F)

E (5

_ Adult mortality I-L-.-

I.._."

U Young produced 4

l"A 14 Total young produced bk 5A ZV a

3A~

31 35 3$

3%

Final Adult Mortality Nole: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

% Mortality:

(V.

Mean Offspring/Female:

I

. (5 0% Reduction from Control:

A. Al.

1000 mg-NaCI/L Survival and Reproduction Data Replicate number Day 1

2 3

4 5

6 7

8 9

10 V Young produced

-7 (O

C C),,

Adult mortality I

L.

i L.

2 Young produced C)

(0) 0 Adult'mortality

\\.

T7 ITT

\\....

U L.-.-

3

'Young produced ID C

Adult mortality U

U U

4 Young produced

_l 14, Adult mortality L

U.

U..

U_

U U

5 Young produced 10 9

I1.

1 "

O l0

/

'3 Adult mortality

.1.

U U

C--

6-Young produced "0

C6 I

Adult mortality L-,

L L

L L.

u LU 7

Young produced, v'S

-1 A

1 14 Total young produced

.3

[30

,31 31 Final Adult Mortality Note: Adult mortality (L = live, D = dead). SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

4.; !

I Concentration:

% Mortality:

0.

Mean Offspring/Female:

30.'

% Reduction from Control:

.106,1.7 Page 9 7 o.f 102

.SOP AT14 - Exhibit AT14.1, revision 04-01-09

EwamwatgmlbnfrnScjbeh.,a Page 4 of 6

.CICR#

c:

..Species:, Ceriodaphnia dubia

-1 2O00'iri aCIIL Survival and Renroduction Data Replicate number Day 1

2 3

4 5

6

7.

8_

9.

10 Youn I gp I rodueed 0

0

"" ""l"Adult mortality 9ig. pr"A dult cctiity C,..

n _

k..

k,.

k.

3

-Young produced 0

75 "

) 0 Adult mortality U-U U

U U

U 4

Young produced Z.

0 T

0

O.

Adult mortality U

U

" I,.

5 Young produced

=

1 Adult mortality I...

6 Young produced

.)

0 0

Adult mortality Q_

U U

i U

U-.U-'.

7 Young produced 44 0%

Tot al young produced d

-'1 0

'l,1

-1

-Lo

-t Final Adult Mortality

\\.

U U

Note: Adult mortality (L = live, D =dead), SB =split brood (single brood split between two days), CO --carry over (offspring carried over with a duce

)uring transfer).

Concentration:

% Mortality:

U-U Mean Offspring/Female:

.16.

't

% Reduction from Control:

.50 7

1400 mg NaCI/L Survival and Reproduction Data Replicate number

Day, 1 1 2

3 1 4 5

6

7.

9 9

10 I

Young produced

-)

Adult mortality L

.[,.

I_,.

tI,_

2 Young produced O

)

O "o

zo I*

O 1 O

Adult mortalitya--

f- ---

U U_ -

\\.

I,__

k..,

3 Young produced

)

O OC)

C)

O C*

Adult mortality (ilbosi bwt d

CO r

orfi 4

Young produced o

T 0I0 Adult mortality 5

Young produced cn fo C Adult mortalityL Surviva. a Rrui Data 6

Young produced Adult mortality L._

L U-L U-U-

7 Young produced Total young produced i-I1..

FnlAdult Mortality C-I K.-

L U

U i

Note: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), tCO = carry over (onspring carried over with adult during transfer).

0 Concentration:

% Mortality:

7.

-.Mean Offspring/Female:

".,°

% Reduction from Control:

.1,&

7-SOP AT 14 - Exhibit AT 14.1, revision. 04-01-09 Page 98 of 102

Environmental Testing Solutions, Inc.

Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test num*ber:

Test damtes:

Reveiwed by:

CdNaCICR #100 November 10-17, 2009 Concentration Replicate number Survival Average reproduction coefficient of Percent reduction from (mg/L NaCI) 12 3

4 5

6 7

8 9

10

(%)

(offspring/femaie) varation (%)

control (%)

Control 36 31 36 33 36 34 35 32 36 31 100 34.0 6.2 Not applicable 600 33

38.

33

,35 36 33 35 32 35 30 100 34.0..

.6.6 0.0 800 34

.34 34 36 31 31 33 35 31 31 100 33.0 5.7 2.9 1000 29 33 30 29 30 31 31 29 28 34 100 30.4 6.2 10.6 1200 20 14 17 10 20 20 13 17

.20 18 100 16.9 20.8 50.3 1400 9

7 9

2 7

12 10 5

6 12 100 7.9 39.8 76.8 Dunnett's MSD value:.

2.604 MSD =

Minimum Significant Difference PMSD:

7.7 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatmret that can be declared statistically significant in a whole effluent toxicity test.,

Lower PMSD bound determined by USEPA (10t percentle) 13%.

Upper PMSD bound determined by USEPA (90&' percentile) = 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Inierlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA.-2-B-01-005.

US Environmental Protection Agency, Cincinnati, OH.

I

-,.4. A EFTS:

%ý Envl~mental TeOt)V SoqfWui4 Inc.

Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:

.11/10/2009 Test ID:

CdNaCICR Sample ID:

REF-Ref Toxicant End Date:.

11/17/2009 Lab ID:

ETS-Envir. Testing Sol0 Sample Type:

NACL-Sodium chloride*:

Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubia,'

Comments:

Conc-mg/L 1

2 3

4 5

6 7

8 9

10 D-Control 36.000 31.000 36.000 33.000 36.000 34.000 35.000 32.000 36.000

. 31.000

.600 33.000 38.000 33.000 35.000 36.000 33.000

.35.000 32.000 35.000 30.6000 800 34.000 34.000 34.000 36.000 31.000 31.000 33.000 35.000 31.000 31.000 1000 29.000 33.000 30.000 29.000 30.000 31.000 31.000 29.000 28.000 34.000 1200 20.000 14.000 17.000 10.000 20.000 20.000 13.000 17.000 20.000 18.000 1400 9.000 7.000 9.000 2.000 7.000 12.000 10.000 5.000 6.000 12.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Mean N-Mean D-Control 34.000 1.0000 34.000 31.000 36.000 6.201 10 34 34.000 1.0000 600 34.000.

1.0000 34.000 30.006 38.000 6.649 10 0.000 2.287 2.604 34.000

,1.0000 800 33.000 0.9706.

33.000 31.000 36.000 5.714 10 0.878 2.287 2.604 33.000 0.9706

1000 30.400 0.8941 30.400 28.000 34.000 6.241 10 3.161 2.287 2.604 30.400 0.8941

1200 16.900 0.4971 16.900 10.000 20.000

.20.771 10 15.015 2.287 2.604 16.900 0.4971

1400 7.900 0.2324 7.900 2.000 12.000 39.783 10 22.917 2.287

.2.604 7.900 0.2324 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.0 1) 0.85725808 1.035

-0.4383847 0.05843284 Bartlett's Test indicates equal variances (p = 0.27) 6.40927744 15.0862722 Hypothesis Test (]-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE.

F-Prob

.

df Dunnett's Test 800 1000 894.427191 2.60422823 0.07659495 1213.54667 6.48518519 8.2E-33 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mg/L-SD 95% CL Skew IC05 853.846154 68.891583 658.291667 950.259615

-0.9158 ICIO 984.615385 37.1605902 889.984375 1018.01841

-0.4734 IC15 1022.22222 10.1708642.1001.41628 1040.13001 0.1120 IC20 1047.40741 9.4426387 1027.71794 1065.06914 0.2104 IC25 1072.59259^9.34744229 1054.19332 1087.34371 0.2270 IC40 1148.14815 11.8528783 1126.04654 1168.22075 0.4312 IC50 1198.51852 16.0574224 1169.42431 1229.62477 0.4948 I.

11-10-09

lET Page 5 of 6 Species: Ceriodaphnia dubia CdNaCICR #1 P00 Daily Chemistry:

I Day BI 0

I I

'-A A,

I

-0

-Analyst

~A; I

Concentration Parameter

- i w !

II I-m PH (s.u.)

DO (rag/L)

Conductivity (Amhos/cm)

CONTROL Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L)

Temperature (0C) w S

U -

n -

1H (S.U.)

DO (mtJL) 4-600 mg NaCI/L Conductivity (umhos/cm) 1441O

1 S3-4-

Temperature (0-144(

-T q

pH (S.U.)

q~~,q 747 11

-~~q DO (mz/L) 800 mg NaCI/L Conductivity

(*mhos/cm)_

q.

q Temperature (0c) l U

U w

Ir pH (S.UX)

5.

4-01 4,814'

'4-W

-1 DO (rag/L) 1000 mg NaCVL.

Conductivity (A hos/cm) 4... 22.

Temperature (TC).

1200 mg NaCI/L Conductivity

-'..hosTmp Z*30 TemperatureL

'2114A.

,4

',OzA1 1A

-c) nHl (S.AJ.'

':~~3

~

q q I q

'4t.

B'3 I 'I~4e IP~q PH (SU).

DO (m2/L) 1400.mg NaCV/L Conductivity.

(gmhos/cm)

-7 u

Temperature (C)

STOCK

, Conductivity JMM M

(gmhoskcm )

I.

ll Initial F

I Initial II Final I

Initial I -Final I

Page 101 of 102 SOP ATI4 - Exhibit ATI4. 1, revision 04-01-09

r' Page 6 of 6 Species: -Ceriodaphnia dubia CdNaCiCR #:

r Day I - :-.1.S 3 :-., - -

I-4 5

6

....... Analyst-II 9.-

It-M, -^2,T V

II 14J!11 concentration Parameter pH.S.U..

1

.50 T.7 3.

DO_(mg1.

1.9 E3 Conductivity (WMxnOSlCM)

JLID CONTROL Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L) 901 Temperature

~b

~~

~

1..~

(0c)

-7 H S.U 7

.ý

~

§33 600 mg NaCIIL Conductivity (imhosI m)

Temperature"IL,

o(T)

Te

.,orq*,

.o 0t*

ta*

j pH (S.U.)

llal,ý

-1.-14 111L 11.1qR I 14I 61 11.4~1Z I

~

41 -'-~

I.......-

4 ~

II. -

rDO (mg/L) 800 mg NaCl/L Conductivity1 (gimhos/cm) j.I AA-

.1

7.

0

'I Temperature i (0c).

m*

I IWOm-m ii l

m pH (S.U.)

, I I-H.~

I i'I.

II I -4 ii 1-o?

I '~pf 1

0 -

~

4 II -~.*~---

0 -.

~-~---

I I DO (reg./L) 1000.mg NaCI/L Conductivity (pgmhoslcm)I

ý32520 z3C0 2530 Temperature (0C)

I*L I

I p~~~H(S.U.).

.5

".1,,

14-BL

-- 1q

.X

,*]

PDO 1(mg/L)

.t 7.5-6

.2.

1 1.200 mg NaCI/L Conductivity 1Temperaurhos/M)

Z090 2

2Temperature u4.0 11.1 M-44 I PH (S.U.)

PAj 7 -7~

uS

'113 1400 mg NaCI/L Conductivity (utmhoslcm) ail 3 12.0;Y 31'40

~4=

Temperature (0C)

..t L:u* L l

8 I

-U I-omn

....... aI

~

h Initial I Final i Initial Final I Initial Final Initial j[ Final I

I imI

n nn i

m I.

Page 102 of 102 SOP ATI4 - Exhibit ATI4t.1, revision 04-01-09

..i; PERMiTTEE NAMEIADDRESS (Oclude Facilty Name/Location ifDifferent)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX.2000 (INTEROFFICE.SB-2A-SCN)

SODDY - DAISY. TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio

, HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR) 6450 1

I PERMIT NUMBER EDISCAR NUMBER From ME NITORING PERIOI YEARP I(

"n LnAY--

ý12 mn YIA n

C AY

-From l 09 1, 12 F 01 7 TO 1 9 12 1 31 MAJOR Form/

(SUBR 01)

OMB F-FINAL LOW VOL. WASTE TREATMENT POND-EFFLUENT

\\pproved.

0o. 2040-0004 ATTN: Stephanie A. Howard

- " NO DISCHARGE GE Z*

NOTE: Read instructions before comoletinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR-CONCENTRATION:

NO.ý FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM.

UNITS ANALYSIS PH SAMPLE 7.2 82 12, 0

13/31 GRAB MEASUREMENT 8

0 00400 1

0 0

PERMT 6.0 90*

T HREE GRiA EFFLUENT GROSS VALUE MINIMUM MAXIMUM W.............EEK GRAB SOLIDS, TOTAL SUSPENDED SAMPLE 56 81 26 6

8 0

5 / 31 GRAB MEASUREMENT 8

19-,

19

00530, 1

0 0

PERMIT

~<

380 1250 LBS/DY

- 71'AQ.00GLWEKY GRAB EFFLUENT GROSS VALUE REIMETMOAVG DAILYMXREQUREMEN EFFLUENT GOSS VALUEMO AVG DAILY MXMOAG DIY X

<6 6

ii9 0

5 /31 GRAB OIL AND GREASE SAMPLE

<56

<60 26 6<6 195 MEASUREMENT 00556'-

1 0

0 PERMIT 190 250 LBS/D`Y 1 5 20 MGIL WEEKLY GRAB EFFLUENT GROSS VALUE MO AVG DAILY MX MO AVG DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 1.196 1.446 03 0

31 / 31 TOTALZ TREATMENT PLANT MEASUREMENT 50050.

1 0 :-0 REQIRMET REPORT EFFLUENT GRXSS VALUE MO A DI MXERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

5PERMIT, SAMPLE MEASUREMENT PERMIT.K REQUIREMENT NA ETJL PI4CPL XCUIEOFFICER Il Certify under penalty of law that this document and all attachments were prepared under my TEEPON DAT NAME/TiTLE PRINCIPAL EXECUTIVEdirection or supervision in accordance with a system designed to assure that qualified personnel

(~~k.§x A

TLPOEDT Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SION & WBN 43 01 1

1 0

Seuya it ie rsiet intormation, the information submitted is,to the best of my knowledge and belief, true, 423_____________________10___01__07_

accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED ORPRINTEDinformation. including the possibility of finý and irmprisonmenttfor knowing violations.

OFCRRAUORZDGET

.AREA NUMBER.

YEA MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he, EP om3301(E 3/9)

Pevous ditons ma be usedi Page 1 of~ ii !ii~

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page I of I

PERMITTEE NAME/ADD6RESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)

SODDY - DAISY. TN 37384 FacilitY TVA - SEOUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATrN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT. (DMR)

TZI 026450 ll 107 G PERMIT NUMBER LDISCHARGE NUMBERI r

YoARmMO -

DAY" Y

DAYTo SFrom l0 1 I011 Tol 09 12 3 MAJOR Form Approved.

(SUBR 0.1)

OMB No. 2040-0004 F -FNAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE D.

NOTE: Read instructions before completinq this form, PARAMETER PH 00400 1

0 0

EFFLUENT GROSS VALUE

><-I QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX FREQUENCY SAMPLE OF TYPE ANALYSIS AVERAGE MAXIMUM -

UNITS MINIMUM AVERAGE MAXIMUM -

UNITS SAMPLE MEASUREMENT I..

8.0 8.7 12 SU 0

7/31 D--AILY GRAB GRAB PERMIT REQUIREMENJT 6.0 9.0 MAiVIAian SOLIDS, TOTAL SUSPENDED SAMPLE 2

19 0

7 / 31 COMPOS MEASUREMENT 19 00530 1

0 0

PERMIT

'.Y.

30 FMGGL1 L

DAILY

. 'OMPOS.

EFFLUENT GROSS VALUE REQUIREMENT DAILY MX-OIL AND GREASE SAMPLE

<6 731 GRAB MEASUREMENT 1*.

0 00556 1

0 0

.PERMIT 44

~~**1 GL~

AL~GA EFFLUENT GROSS VALUE REQUIREMENT DAILY MX PHOSPHORUS, TOTAL (AS P)

SAMPLE

......0 19 MEASUREMENT 00665 1

0 0

PERMIT 1.0

'MG/L DAILY*

MPOS EFFLUENT GROSS VALUE REOIRt~(ENT DAI Y Mx COPPER, TOTAL (AS CU)

SAMPLE 0.**023

19.

0 7 / 31 COMPOS MEASUREMENT 01042 1

0 0

'KPERMIT>'

,*'.0**

MG/

DAILY OMP*

EFFLUENT GROSS VALUE REQUIREMENT~

DAL M.. X GI ALYCM IRON, TOTAL (AS FE)

SAMPLE

- - 0.

14 1'9 0

7 / 31 COMPOS MEASUREMENT 01045 1

0 0

PE RMITK K>

1.0 MGIL DAILY ICOMPOS EFFLUENT GROSS VALUE REQUIREMENT

.DAILY MX' FLOW, IN CONDUIT OR THRU SAMPLE 0.021

.0.034.

03 0

7/31 CALCTD TREATMENT PLANT MEASUREMENT 50050 1

0 0

PEýRMIT REPORT REPORT MGD

~

r#DAILY CALCTD

,EFFLUENT GROSS VALUE

[REQ()UIREME NT LMO AVG DAILY MX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my it

.-. i TELEPHONE DATE

"-irection or supervision in accordance with a system designed to assure that qualified personnel

/

Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons.directly responsible for gathering the Environmental Manager SQN & WBN 423.

843-7001' 10 01 07 information, the information submitted is to the best of my knowledge and belief, true.

42_

_843_7001_10_01 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

AREA.

NUMBER CME TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT (ernc NME YEAR MO DAY rnrl)r"I

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattaclimentsniet' The Metal Ponds (Outfall 107) were discharged directly to the LVWTP (Outfall 103) on 12/3/09, 12/8/09, 12/10/09, 12/15/09, 12/16/09, 12/17/09, and 12/22/09. No phosphorous bearing cleaning solutions were used."

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page I of 1

PERMITTEE NAME/ADDRESS

[Include Facility Name/Location if Different)

Name

, TVA-SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000

- (INTEROFFICE SB-2A-SN)-

SODDY - DAISY. TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMRR TN0026450 110 G PERMIT NUJMBER DISCHARGE NUM3BER Fro "MNIT("0RINi P:RIOD YEAR MO I DAY-1 YýR ý MQt IDAY FromI 09 L 12 1 01. 1 To.. 09 OS 2

3 MAJOR Form Approved.

(SUBR 01)

OMB No. 2040-0004 F -FINAL RECYCLED COOLING WATER EFFLUENT NO DISCHARGE NOTE: Read instructions before corftoletinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EXi OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIs TEMPERATURE, WATER DEG.

SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 Z

0 0

"'PERMJT i!

DEG C "38.3 DEGC.C DAILYG GRAB.24 INSTREAM MONITORING REUIEMNT PH SAMPLE 12 MEASUREMENT EFFLUENT GROSS VALUE MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1

0.

0 PI E RMIT,

- - - i*

30 i

MG/L DAILY COMPOsjS EFFLUENT GROSS VALUE REURMND AILY MX OIL AND GREASE SAMPLE

.*1.

MEASUREMENT 19.

00556 1

0 0

PERMIT

,15 MG/L

-~DAILY GRAB EFFLUENT GROSS VALUE

.FOLIREMENT i: y AL FLOW, IN CONDUIT OR THRU SAMPLE 03***

TREATMENT PLANT MEASUREMENT 50050.

1 0

0 REPORT i

E-REPORT MGDE*.

D CALCTD EFFLUENT GROSS VALUE MO AVG DAILY MX CHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 50060 1

0 0

PERMIT

'.0.10 GLWEKLY GRAB-4 EFFLUENT GROSS VALUE REURMN

'DAILY MX SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE. PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my "

TELEPHONE" DATE direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church.

properly gather and evaluate the information submitted. Based on my inquiry of the person or Persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01 07 Sequoyah I Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED OFFCOERDORAUTHORIZEDAGENTAREANUMBERYEARMO_

DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference ?//attachments /e76 No Discharge this Period EP For 3301(E 39)

Peioseiinmyb sd ae1o EPA Form 3320-1 (REV 3/99)

Previous editions may be used Pagel1 of I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)

.SO__DDY - DAISY. TN. 37384--

Facility TV

- SEQUOYAH NUCLEAR PLANT Locatio. HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

TN0026450 110 T F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved.

OMB No. 2040-0004 F _

~MnrtwTnCW1rur DCVIrfl 7 EFFLUENT From] 09 122011 T

311 NO DSCHARGE NOTE: Read instructions before completinq this form.

PARAMETER J

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO:

FREQUENCY SAMPLE EX-OF TYPE AVERAGE

MAXIMUM, UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE 2

CERIODAPHNIA MEASUREMENT TRP3B 1

0 0

-PERMIT

,,4..2 PERCENT SEMI Z

-OmbS EFFLUENT GROSS VALUE IELRMN ibi.MiNIMUM ANUA IC25 STATRE 7DAY CHR SAMPLE 23 PIMEPHALES" MEASUREMENT 23 TRP6C 1

0 0

/.

PERMIT.

45....

PERCENT

.SEMI.

COM0OS EFFLUENT GROSS VALUE MEINUUREMEM iK.$i-ANNUAL SAMPLE MEASUREMENT

~PERMIT,,

REQUIREMENT.

SAMPLE MEASUREMENT PERMIT REAUIREMENT' SAMPLE MEASUREMENT YPERMIT:

~REQUIREMENT SAMPLE MEASUREMENT,

-PERMIT.

SAMPLE MEASUREMENT

<PERMIT-R5E,)UIREMENT.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my.

TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified person_

__el_

Christopher R. Church properly'gather and evaluate the information submitted, Based on my inquiry of the person o, persons *vho manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WBN 8

1 information, the information submitted is, to the best of my knowledge and-belief, true,

__423

"_843-7001,_

1_0 01 07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

T OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he.

No Discharge this Period EPA ForrTi i320- 1 (REV 3199)

Previous editions may be used

-:Pagel1of I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)

SODDY - DAISY. TN 37384.

Facilitv TVA - SEQUOYAH NUCLEAR PLANT Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (tDMR)

ZTN(0Z45 116 G PERMIT NUMBER DISCHARE NUMBER Fro NITtRING PERIOr)D SYEAR }

eI DAY L JYEAR J MO DA' From o 9T12 1011 To[ 09T 12 311 MAJOR Form Approved.

(SUBR 01)

OMB No. 2040-0' F - FINAL BACKWASH EFFLUENT NO DISCHARGE NOTE: Read instructions before comoletina this form, 004 ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)

SAMPLE 0**

0 1 / 31 VISUAL MEASUREMENT 0145 1

~R EQUREMENT'

.'~*

- - - "'~

REPORT PASS=O

, K, SEE VISUAL EFFLUENT GROSS VALUE

,REIEMN

'i MO TOTAL FAIL=1 9'.PERMIT..

OIL AND GREASE VISUAL SAMPLE 0

94 0

1 /31 VISUAL MEASUREMENT 84066 1

0 0 V PERMIT i****iM*

REPORT YES=Io i

1 SEE VISUAL EFLET RS VLEREQUIREMENT

,NO4 PERMIT.

SAMUNTGRSLEAU MO TOTAL

=

.~

,.'PRI SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT

'PERM[IT' REQUIREMENT SAMPLE MEASUREMENT PERMIT' REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER tI Certify under penalty of law that this document and all attachments were prepared under mY.

.i

-)*

I i

TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel DATE.,,,

Christopher R. Church property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly, responsible for gathering the Environmental Manager.SQN & WBN 423 843-7001 10 01 07 h

information, the information submitted is, to the best of my knowledge and belief, true,

_ ___843400___0___ __07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE linformation, including the possibility of fine and imprisonment for knowing violations.

O TYE RPITD-OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTEDCOD COMMENTS ANDEXPLANATION OF ANY VIOLATIONS (Reference allattachments hel Operations performs Visual inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3199)

. Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name :.

TVA-SEOUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A-SON)

SODDY - DAISY. TN 37384 FacilitY TVA - SEQUOYAH NUCLEAR PLANT LoCatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM, (NPDES)

DISCHARGE MONITORING REPORT (DMR)

"" TNO026450 1i" "17 G 1

PERMIT NUMBER

_DISCHARaGE NMBER From MONITOjRINGPRIO 1

I Y EA R

M (' I D AY

I Y A I ý L lý M r) I nA Y I FromI 09 112 11 01 1TOl 09 112-131{

MAJOR (SUBR 01)

F-FINAL BACKWASH EFFLUENT Form Approved.

OMB No. 2040-0004 ATTN: Stephanie A. Howard NO DISCHARGE, NOTE: Read instructions before comoletinq this form.

PARAMETER OUANTITY OR LOADING 1

QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

'EX OF TYPE*

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)

SAMPLE 0

9A 0

1 /31 VISUAL MEASUREMENT 01345 1

0 0

~PERMIT7.

~

- - ~

,Wo****.,,

RPR PASS=0 K

SEE, Vlt EFFLUENT GROSS VALUE REOUIREMEN

',.i..

1.

'M<

FAIL=I PERMIT EFFLU NT G OSS ALUEM O TOTAL OIL AND GREASE VISUAL SAMPLE 0

94**

0 1 /31 VISUAL MEASUREMENT EFFLUENT84066 GROSSVALUE 1

0 PERMIT EN REPORT.

Y.ES=

SEE VISUAL EFLETGOSVLEREQUIREMENT.

MO TOTAL N =

PEMI SAMPLE MEASUREMENT j7PERMITh...............

REQUIREMENT SAMPLE MEASUREMENT PERMIT.

REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT

.~PERMIT.

REQUIREMENT SAMPLE MEASUREMENT

PERMIT, REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my/I f

TELEPHONE DATE

,direction or supervision in accordance with a system designed to assure that qualified personnel Christopher R. Church properly gather and evaluate the information submitted. Based on my inquiry of the person or

-I

.~-

persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SON & WBN 42 8

S information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01_*_07 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED 0 -______

ORATOIZDAET ARA_

_R YARM.DY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments he" Operations performs visual inspections for floating debris and oil 'and grease during all backwashes.

EPA Form 3320-1 (REV 3/99)-ý Previous editions may be used Page I of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEOUOYAH NUCLEAR PLANT Address P.O: BOX 2000 (INTEROFFICE SB-2A-SON)

SODDY - DAISY, TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT-Locatio HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form'Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

OMB No. 2040-0004 EITNU2645ER 1

F -FINAL L PERMuITNUMBERJ LPJ511ARGE N UB WASTEWATER & STORM WATER ftlý I

EFFLUENT I

~EI¶ILIJ I

ATTN: Stephanie A. Howard YEAR IMO AI YEAR MT DAY NO DISCHARGE From 09 12 01 N

eTot ti b

c1otnhfr NOTE: Read.irnstructions *before comipletincl this form.

PARAMETER OUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)

SAMPLE MEASUREMENT 1

00300 1

0 0

.PERMIT 2..

MG/L TWICE/

GRAS EFFLUENT GROSS VALUE AI,-Y M, WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 19 00530 1

0 0

PERMIT 1

11 TWICE/

EFFLUENT GROSS VALUE RE, Ri.

,....A......

SOLIDS, SETTLEABLE SAMPLE

.25 MEASUREMENT 25 00545 1

0 0

EMT....~*~*

- - A*

9****'

,'1.0 MUL ONCE/

'GRAB EFFLUENT GROSS VALUE' REIU IREMET M

FLOW, IN CONDUIT OR THRU SAMPLE

- - - - ONTH TREATMENT PLANT MEASUREMENT 03*

50050

.1 0

0 PERMiT<

REPORT

ýREPORT MGD

.~***.

- - $.ONCE/

ESTIMA EFFLUENT GROSS VALUE REQUIREMENrtT MO AVG DAILY MX

.ATCH i*

'REASUIREMENT SAMPLE MEASUREMENT F1ERMIT REQUIREMENT' SAMPLE MEASUREMENT tPERMIT

'REQUIR~EMENT' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

/

TELEPHONE DATE

-direction or supervision in accordance witi a system designed to assure that qualified personnel AT I

1 IVJL C.

Ohristopher R. Church properly gather and evaluate the information submitted. Based onmy inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Manager SQN & WBN Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-7001 10 01 07 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ICODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments het During this reporting period, there has been' no flow from the Dredge Pond other than that resulting from rainfall.

EP.om32-RV399 rvoseiin a be sedPae.

f EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page.1 of 1

ML100150093

Contents

Text

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

Navigation menu

ML100150093

Text

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

Navigation menu

Search