Text

Discharge Monitoring Report for February 2009
	ML090760607
Person / Time
Site:	Sequoyah
Issue date:	03/12/2009
From:	Clearly T; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML090760607 (112)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 March 12, 2009 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 h Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Patrick Cromer:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2009.

Enclosed is the February 2009 Discharge Monitoring Report for Sequoyah Nuclear Plant certified by the duly authorized representative. If you have any questions or need additional information, please contact Ann Hurt at-(423) 843-6714 or Stephanie Howard at (423) 843-6700 of Sequoyah's Environmental staff.

Sincerely, Timothy P.

leary Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 200 (INTEROFFICE S B-2A) _

SODDY - DAISY TN 37384 Facility TVA-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPlES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

TN0026450 L

o 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER]

DIFFUSER DISCHARGE MONITORING PERIOD EFFLUENT Form Approved.

OMB No, 2040-0004 YEAR MO DAY IYEARI MO IDAY From 09 102 01 To09 02 28-NO DSCHARGE

Z

~ltead inlstructionls before complJetingq this formi.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS.

ANALYSIS TEMPERATURE, WATER DEG.

SAMPLE 11.6 04 0

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.i DAILY MX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ttallAjy TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel

,oT HD Timothy P. Cleary properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 09 03 10 information, the information submitted is, to the best of my knowledge and belief, true, Princial_

Enironm ntalnginee_423_43-670_09__03_1 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation.:Theh following information is included in an attachment: 1. CCW data 2. Veliger monitoring data EPA Form 3320-1 (REV, 31§9)

Previous editions may be used Page 1 of 2

DMR Attachment

. CCW Data

CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst

Method, 02/11/2009 @ 1058 0.13 mg/I 02/13/2009 @ 1136 DAP TN EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 02/11/2009 @ 1100

<0.11 mg/I 02/13/2009 @ 1146 DAP TN EPA 8015

Sample Date Mean

%of Settlers WatMean#

of NOTES: %

Mean # of WtrTm.Water Temp.

SUB Water Temp Sample Date Asiatic Wate Temp.*LOCATION Gravid Asiatic.

COLLECTED BY ZM/m

)Casm3

(,C)

LOCATION Ca 11/21/2008 11/24/2008 12/4/2008 12/8/2008 12/15/2008 12/22/2008 12/29/2008 01/09/2009 01/16/2009 01/22/2009 01/30/2009 02/06/2009 02/13/2009 02/20/2009 02/26/2009 0

31 0

0

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0 0

12

.11 10 9

9 9

9 8

7 7

9 6

11 9

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0 0

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9 9

9 8

7 7

,9 6

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9 Inplant Inplant Inplant Inplant Inplant In plant Inplant Inplant Inrplant Inplant Inplant Inplant Inplant Inp1ant Inplant RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW RCW Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Adcock / Bryant Adcock / Bryant Adcock Bryant Adcock Bryant Love Bryant Bryant

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 INTEROFFICE SB-2A)

SODDY - DAISY TN 37"384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard Different)

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

LI TN0026450 I

1017G F - FINAU PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE MONIT RING PERIOD EFFLUENT Form Approved.

0MB No. 2040-0004 PARAMETER I YEAR MO DAY YEAR MO I DAY NO DISCHARGE I

From 09 02 01 To[

02 28 I L

NOTE: Read instructions before completing this form.

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY! SAMPLE

'_EX OF I

TYPE IANALYSIS AVERAGE MAXIMUM UN M

U AVERAGE MAXIMUM UNITS J" I.

i 0.016 0*,030 0

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1 irertion or sunervsion Timothy P. Cleary Sequoyah Site Vice President of law that this document and all attachments were prepared under my in accordance with a system designed to assure that qualified personnel aluate the information submitted. Based on my inquiry of the person or he system, or those persons directly responsible for gathering the ation submitted is, to the best of my knowledge and belief, true, I am aware that there are significant penalties for submitting false he possibility of fine and imprisonment for knowing violations.

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT Jinfurmation.

TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

The following injection occured: Biodetergent 73551 (max. calc. conc. was 0.021mg/L--limit 2.Omg/L)

EPA Form 3320-1 (REV 3/99)

" Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

_(IN!TEROFFICE SB.2A SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR For DISCHARGE MONITORING REPORT (DMR)

OM (SUBR 01).

Of TN0026450

ý[

101 T F FIFINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 I MONITORING PERIOD EFFLUENT m Approved.

lB No. 2040-0004 IYEARI MO DAY YEAR MO DAY From To 09 02 28

.* NODISCHARGE NOTE: Read instructions before comoleting this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I NO.

FREQUENCY1 SAMPLE IEX iANALYSIS J

E OF I

TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I _

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>4...4 SAMPLE MEASUREMENT SAMPLE MEASUREMENT FEUPERMT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I,

TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 03 10 Seqioyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity was sampled February 8-13: 2ý009. Report is attached EPA Form 3320-1 (REV 3/99)

.Previous editions may be used

.Page 1 of 1

March 9; 2009 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO'.TN0026450, COMPLIANCE TOXICITY TESTS, FEBRUARY, 2009

A-tached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results.

of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected February 8-13, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

Call me at. (256) 386-2755 if you have any questions or comments following your review' of the report.:

Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):

Sherrard, R. M., PSCI X-C Files, ER&TA, CEB IB-M SQN February 2009m

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT

, INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: March 9, 2009 I. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1579

6. Receiving Stream: Tennessee River (TRM 483.6)

7.

1Q10: 3,491

8. Outfall Tested: 101"

9. Dates Sampled: February 8-13. 2009

10. Average Flow on Days Sampled (MGD): 1582, 1597, 1596

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: February 10-17, 2009

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC25 = 45.2%

17. Test Results: Outfall 101: Pimephales prom elas: IC25 > 100%

Ceriodaphnia dubia." IC25-> 100%

UV treated Outfall 101: Pimephales promelas: IC25 > 100%

Page 1 of 98

18. Facility

Contact:

Ann Hurt Phone #: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

'21: TVA

Contact:

Cynthia L. Russell

-Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected February 8-13, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 2 of 98

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite.

3. Sample, Information:

Date Date Date (MM-DD-YY)

(MM-DD-YY)

Arrival Initial (MM-DD-YY)

Sample Time (ET)

Time (ET)

Temp. I TRC*

Time (ET)

ID Collected Received

(°C)

(mg/L)

Last Used By 101 02-08-09 0921 to 02-09-09 1554 1.7, 1.9

<0.10 02-10-09 1110 02-09-09 0821 02-11-09 1206 02-08-09 0940 to 02-10-09 1110 Intake 02-09-09 0840 02-.09-09 1554 2.4

<0.10 02-11-09 120 02-09-09 0840 02-11-09 1206 02-10-09 0915 to 02-12-09 1030 100815 02-11-09 1553 2.2, 1.6

<0.10 2-13-09 111 02-10-09 0936to 02-12-09 1030 Intake 02-11-09 0836 02-11-09 1553 3.1

<0.10 02-13-09 1110 02-14-09 1012 02-12-0.9 0936 to0210902 101 02-1-09 036 02-13-09 1531 1.5, 1.5

<0.10 02-15-09 1014 02-13-09 0836 02-16-09 1011 02-14-09 1012 02-12-09 0842 to 02-15-09 1014 Intake 02-13-09 0742 02-13-09 1531 0.7

<0.10 02-15-09 1014 02-16-09 1011

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmned to test temperature (25.0 + 1.00C) in a warm water bath.

Aliauots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured bv Emperor Aquatics, Inc.) for 2 minutes.

Page 3 of 98

Pimepthales promelas Test Organisms:

Ceriodaphnia dubia In-house Cultures

1. Source:

Aquatox, Inc.

2. Age:

Test Method Summary:

1. Test Conditions:

19 to 19.17 hours1.967593e-4 days 0.00472 hours 2.810847e-5 weeks 6.4685e-6 months old

<24-hours old Static, Renewal Static, Renewal

2..Test Duration:

7 days Until at least 60% of control females have 3 broods' Moderately Hard Synthetic

3.

Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5. Organisms per Replicate: 10

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101

8.

Test Temperature: Outfall 101:

10 02-10-09 111OET 02-10-09 1100 ET 02-17-09 1030 ET 02-17-09 1046 ET Mean = 24.6°C (24.1 - 24.90 C) 02-10-09 0830 ET 02-16-09 0913 ET Mean = 24.8°C (24.5 - 25. 1°C)

Test Temperature: UV-Treated Outfall 101:

Mean = 24.6°C (24.2 - 25.1LC) 9.

Physical / Chemical Measurements:

Alkalinity, hardness. totalresidual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for DH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4 of 98

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1.

Results of a Pimephales promelas Chronic/!7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted February 10 - 17, 2009 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2

3.

4 5

6 7

Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100.

100 100 45.2%

100 100 100 100 100 100 98 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Mean Dry Weight (mg)

Test Solutions (replicate number)

(% Effluent).

. 2 3

4 Mean Control 0.644 0.678 0.597 0.563 0.621 11.3%

0.630 0.628 0.616 0.683 0.639 22.6%

0.670

.0.679 0.612 0.743 0.676 45.2%

0.614 0.619 0.693 0.525 0.6_13 72.6%

0.577 0.522 0.658 0.579 0.584 100.0%

0.605 0.742 0.593 0.534 0.619 Intake 0.622 0.612 0.599 0.553 0.597 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0: TUc = 100/IC25 Page 5 of 98

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphnia &ubia. Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted February 10 - 16, 2009 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 1

2 i

3 1

(%Effluent) 1 3

j J 6 7

Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 100 100 100 100 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)

Tet Soluetis Data (replicate number)

(%Effluent) 1 2

3 4

5 6

7 8

9 10 Mean Control 34 31 31 30 34 35 29 31 33 32 32.0 11.3%

33 33 35 31 35 34 30 32 31 32 32.6 22.6%

35 31 33 32 35 35 31 35 33 35 33.5 45.2%

35 35 33 32 36 35 35 33 35 33 34.2 72.6%

35 34 37 34 35 38 36 37 36 34 35.6 100.0%

37 34 39 38 36 39 36 35

39.

36 36.9 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0: TUc = 100/IC25 Page 6 of 98

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a.Ceriodaphnia dubia *Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted February 10 - 16, 2009 using water from Intake Percent Surviving Test (time interval used - days)

Solutions

('% Effluent)

I 2

3 4

5 6

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/6 days)

EData (replicate number)

(%Effluent) 1 2

3 14 5

6 71 8

9 1 10 Mean Control 32 31 31 33 33 31 33 32 31 30 31.7 Intake 34 37 35 33 37 40 35 34 37 35 35.7 1C25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa 100/LC5o: TUc = 100/IC 25 Page 7 of 98

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted February 10 - 17, 2009 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(%Effluent) 1 2

3]

4

[5 6

7 Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100.

45.2%

100 100 100 100 100 100 100 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 TMean Dry Weight (mg)

Test Solutions (replicate number)

(% Effluent)

-1 2

J 3

4 Mean Control 0.611 0.630 0.610 0.665 0.629 11.3%

0.642 0.720 0.729 0.747 0.710 22.6%

0.664 0.740 0.636 0.663 0.676 45.2%

0.716 0.622 0.516 0.577 0.608 72.6%

0.642 0.571 0.639 0.676 0.632 100.0%

0.626 0.654 0.647 0.688 0.654 Intake 0.567 0.601 0.586 0.717 0.618 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

95%/ Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 1OO/LC5 0: TUe = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

I Species Date I Time I Duration 1 Toxicant 1 Results (IC 25 ) I Pimephalespromelas February 10- 17, 2009 1130 Ceriodaphnia dubia February 3 - 9, 2009 0750 KC1 0.83 g/L NaCl 1.05 g/L Page 8 of 98

PHYSICAL/CHEMICAL

SUMMARY

Water.Chemistry Mean Values and Ranges forPimephalespromelas and Ceriodaphnia dubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed February 10-17, 2009.

Test Samo!e ID Temperature ('C)

Dissolved Oxygen (mg/L) pH (S.U.)

Conductance Alkalinity Hardness' TotaiResidual Initial Final Initial Final Initial Final (pmlios/cm) (mg/L CaCO)

(mg/L CaC0 3 )

Chlorine (mg/L) control 24.7 24.5 7.7 7.4 7.78 7.69 324 63 95 24.6 -

24.8 24.2 24.7 7.5 8.2 6.6 7.7 7.69 -

8.01 7.44 7.81 306 345 62 64 92 98 24.7 24.5 7.9 7.4 7.85 7.66 310 11.3%

24.7 -

24.9 24.1.- 24.7 7.7 8.4 6.6 7.8 7.80 -

7.88 7.30

- 7.80 300 332 24.8 24.5 7.9 7.4 7.86 7.67 292 22:6%

24.7 -

24.9 24.3 24.7 7.7 8.4 6.2 -

7.8 7.81 -

7.89 7.38

- 7.79' 282 300 42%

24.8 24.4 7.9 7.4 7.87 7.67 269 45.2%

24.7 -

24.9 24.2 -

24.5 7.7 8.5 6.3 7.7 7.81 7.90 7.38 -

7.81 262 275 26%

24.8 24.4 8.0 7.4 7.88 7.70 240 72.6%

24.7 -

24.9 24.2 -

24.6 7.7 8.5 6.3 7.7 7.81 7.92 7.39

- 7.83 233 245 24.8 24.4 8.0 7.3 7.89 7.71 21 1 73 85

< 0.10 10 0.0%

24.7 -

24.9 24.2 -

24.6 7.7 8.5 6.3 7.7 7.81 7.94 7.40

- 7.83 204 -

218 70 75 82 86

<0.10 -

<0.10

.24.8 24.4 8.0 7.4 7.90 7.71 211

, 73 86

<0.10 Intake 24.7 -

24.9 24.2 24.5 7.7 8.5 6.4 7.7 7.85 - 7.95 7.40 - 7.84 206 -

218 72 74

. 82 92-

<0.10 -

<0.10 Control 24.7 24.8 7.7 7.8 7.79 7.81 321 63 95 24.5 -

24.8 24.6 -

25.0 7.5 8.2 7.5 -8.2 7.69 -

8.01 7.75 - 7.89 306 325 62 64 92 98 11.3%

24.8 24.8 7.9 7.8 7.85 7.80 309

.2 1 _"3%

24.7 -

25.0 24.6 -

24.9 7.7 8.4 7.6 8.2 7.80 -

7.88 7.75.-

7.88 300 332 22.6%

24.8 24.9 7.9 7.8 7.87 7.80 291 24.7 -

25.0 24.8 -

25.0 7.7 8.4 7.6 8.2 7.81 7.89 7.74 - 7.86 282 300 45.2%

24.8 24.8 8.0 7.8 7.88 7-82 268 24.7 -

25.0 24.7 -

25.1 7.7 8.5 7.6 8.2 7.81 7.90 7.75 - 7.87 262 275 24.8 24.8 8.1 7.7 7.89 7.82 239 247 -

25.0 24.7 -

24.9 7.8 -

8.5 7.5 8.2 7.81 -

7.92 7.75 - 7.87 233 245 100.0%

24.9 24.8

.8.0 7.7 7.90 7.84 21 1 73 85

< 0.10 24.7 -

25.1 24.6 -

24.9 7.7 8.5 7.5 8.0 7.81 7.94 7.76 - 7.89 204 -

218 70 75 82 86

<0.10 -

<0.10 Intake 24.7 24.8 8.1 7.7 -

7.91 7.85 210

73.

86

< 0.10 24.6 -

24.9 24.6 -

25.0 7.7 8.5 7.6.-

8.1 7.85 -

7.95 7.76 - 7.90 206 217 72 74 82 92

<0.10 -

<0.10 Overall temperature CC)

Pimephales promielas Ceriodaphnia dubia Average Minimum Maximum 24.6 24.8 24.1 24.9 24.5 25.1 Page 9 of 98

PHYSICAIU/CHEMICA[

SIUMMARY Water Chemistry Mean Values and Ranges for Pimephales promelas Tests, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed February 10-17, 2009.

Test Sample ID Temperature ('C)

Dissolved Oxygen (mg/L) pH (S.U.)

Conductance Alkalinity.

Hardness Initial Final Initial Final Initial Final (jimhos/cm)

(mg/L CaCO 3) (mg/L CaCO 3)

C r

24.7 24.6 7.9 7.4 7.83

. 7.54 310 62 91 Control 24.6 -

24.8 24.5 - 24.6 7.6

-8.5 6.7 7.8 7.74 7.91 7.06 -

7.73 303 318 61 63 90 94 24.8 24.5 7.9 7.3 7.76 7.54 304 11.3%

24.7 -

24.9 24.3 - 24.6 7.6 8.5 6.6 7.6 7.15 -

7.91 7.04 -

7.76 296 313 26'24.8 24.4 7.9 7.3 7.76 7.55 293 25.0 24.2

- 24.7 7.6 8.5 6.5 7.8 715 7.91

-7.04

- 7.75 287 303 24.8 24.4 7.9 7.4 7.77 7.57 271 45.2%.

24.7 -

25.0 24.2 - 24.7 7.7 8.5 6.5 7.8 7.15 7.91 7.03

- 7.74 263 279

-"2.6%/

24.8 24.4 8.0 7.3 7.77 7.58 239 24.7 -

25.0 24.2

- 24.7 7.7 8.5 6.4 7.7 7.15 7.92 7.02 -

7.78 234 245 1000%

24.9 24.4 8.0 7.4 7.78 7.59 211 71 85

.100_0%

24.7 -

25.1 24.2 - 24.6 7.7 8.4 6.4 7.7 7.14 7.93 7.02 -

7.77 204 218 70 72 84 86 Intake 24.9.

24.5 8.0 7.2 7.75 7.58 7 20 9 72 85

____________24.7 25.0 24.2 24.7 7.8

-8.7

.6.4

-7.4 7.14

-7.92 6.99 7.83 197

-216 69

-74 84

-86 Overall temperature CC)

Piniephales prome las Average 24.6 Minimum Maximum 24.2 25.1 Page 10 of 98

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected February 8-13, 2009, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were

> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

F"athead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 11 of 98

Appendix A ADDITIONAL TOXICITYTEST INFORMATION

SUMMARY

OF.METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None Page 12 of 98

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 2510 B.

'7. Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test,.and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KC1 or NaCl.

2.

Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas.

Sodium Chloride (NaC1 crystalline) for Ceriodaphnia dubia.

3.

Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0was used for statistical analyses.

Page 13 of 98

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. -Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater. Organisms, EPA-821 -R-02-013 (October 2002).

3.

Standard Methods for the Examination of Water and Wastewater, 2 0th Edition, 1998.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing

/ Solutions, Inc (most current version).

Page 14 of 98

Sequoyah Nuclear Plant Biomonitoring February 10 - 17, 2009 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 15 of 98

Table B-1 Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 13, 2009 Date Sidii am Towerbrom PC L-212.2 PCL-401 CL-3.63*

Cuprostat-H-i

YpoehitO mg/L mng4L mg/L mg/I,

PF MngL TRC Phospb:te Copolymer mg/L Q.

.TRC

.Azole 03/12/1998 0,016--

03/13/1998

.0.015-03/14/1998 0 03/15/1998::

0.03 03/16/1998 0.013 03/17/1998 0,020 03/18/1998 1001 09/08/1998::'.

0,0l5

.0.005 09/09/1998:.::..1-0,003 30.011 Si((i~i}

!iiii~li{,,i{,i {

ii{

iii~iiiiiii~lii,{i.

09/10/1998:-

00::

0.021 09/11/1998 t 3:.

. 5 5 0.019 09/12/1998::<. 00....0..44 0.015 09/13/1998

<0001......4

)u 0.015 09/14/1998.

.01 02/23/1999.

0.01 02/24/1999... ~

02/25/199

...... 2.........

02/26/1999 02/2 7/1999

.0.0

}i:**~

:*:i 02/28/1999 08/18/ 1999......

I.

0.....0 08/19/1999 0012....6.

0.024 08/20/1999 023 i[

7Q*

0.024 09/ 1 /199

!:iii:ill}:i::::i:i:{::i~

~it* i:i::::i:)ii::iiiiiii!

~ii!!i

~ ig ii~iiii~iiii*..

.9.............

08/18/1999 02215

.69..

0.024 0.

08/22/19999 0.0122

. 0 0.024

i::i-08/23/1.999 0.025 0.068 0.024

.;0 08/24/1999 016.020*

0.023..............

U

)I!**

............................... * :.!~iiii~i!ii~i*

.i~ ii*# * !:

i)::::i:iiii::ii::i~

iii~i:iii:i.

0 8 1 / 9 9 :::*:{i{::!!!!i:::}:}:.i*:{:iii:!::!::i:::ii{:iiii 0 0 5 ii{iii~i!

! 6* iiii 0 0 4

" ::::::::::::::::::0.0 16:ii~iii i

il!!il...,.......#

108/24/1999[

0.2.023s68~;

i i:*:iiii~i*::5:*:ii~~*)~

Page 16 of 98

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 13, 2009-Date

. Sodiit i

Towerbrom P3.-222 i PCL-401 CL-363 Cuprostat-M130I1v Hypochii*

rite~i mgb mLg/bi}iiiii* ii~iiiii mg/L mg/liiii iiiii:

PF mg/b*!i~

i mg/L TRC h pb C oyer D AI mgLut 01/31/2000

<~ii{ii{iiii~}((ii~ii!ii~ii}ii{i)i 0.01102~ ii~iii{i

{i((ii~~:

0.0268.

0

.i:*#*~*:i*:::i:i:)

i{i~l{i((!**{!{

02/02/2000 {iiiii{i;ii:i~i*iiiiiiiiiii((iii~ii}=

0.028 0.026**~

i i~i~i~** 0.009

!Y~ii t7~ i*i06 02/0/200

.00 0.009...

072 80

/2000 7

iiii~iiiiiiiiiiiiiii}iil~iiiiiiiiii

< 0.088 iii::iiiii:* * ::iii;ii 0.0530.018*:ii!:*:*:i:**:ii*::*::*:

- i:*:*:i:*~~

07/31/2000 *iiiii**iii!!ii!ii*iiiii:iiiiiiii!iiiiii 0.0152 i.0.i0!*:i!*!i i 55.**!iiiiiii 0.019 0.006:::::::::::::::::::::::::::::::::::::::::

08/01/2000

O.0141 0.055iiiii i~iii~iii 0.019 0.00.5 iii!ii

.0 iiiiiiiiii:{iiiiiii 12/11/2000 *i**!;i*ii**

ii!;ii*ii{ii

< 01438

.9.025iii}*iN

~~i~iiiii 0.020 0.005iiiiii~iiiil{iilii iiiiii~ii!*iii;)ii 12/13/2000...

.0120...

050.2 12/1/200 010..00S.000 12/316/2000

<.00362 0.2 0.0209 12/017/2000

.0.036110 0.0208 08/026/2001 0172 0'. 2~6 0.021 60.)

08/27/2001.......

0

09608:

0.021 0.......

08/29/001....0094.....

0.2 0...I......

08/304/2001 01230060 0.021 1090 11/25/2001 0044...

11/6/00 01192 0 0.2 0.020090 01/20/2000 ::

< 0 002 0,027 0.019 07 07/28/2001

0089

.20

<0.0057 00550.019..........

1211/29/ 001 0132{*((i{i*iiii}((ii{*i*~iii~ii 0.01424

.ii((ii~

~ iii~((ii 0.020 0,007..iiiii::::::::::::::::i:::::::::::::

11/30/2001.

.004.......0.0 12/12/2001 0.0120 2

0:

0.020

+.: +.:+..

.:+

01/21/2001 00880004 0.027 0

3 0.01 0

01 /21 /2001

< 0.0013

.P.054

0.021 07/30/2000

<((iiiii~i~ii!i)ii((ii~ii{iiii{iii~

< 0.012076 iiiii!ia~ li~ii~i 0.0219

!!~ii*i~((ii{}i~i{~iii(({

07/3 1/2000

<i((~iiiii}~iii~~iiiiiiii~{iiiiii{i

< 0.0 152 0.019 0iilii~

006.i:i

{Sii:~::{ii{:i~?{

. = : =.....

1 2 0

/ 0 1 :

0. 0 2

- ii:*:*::*:ii*:i4

ii:**:*~*::*:***

i~? !

12/1.....................................

~~i!*iiiiii~iii 12 1 / 0 1 iiiiiii)iiiii~iiiii iiii~iiiiiiiiii::iii

<. 0 4

0:.0:::: ::::::::::::::4 3::::::::::::::::0.0 2 0:::::::::::::::::::::

12/12/2001

~i~i~~iii~{*

i*i~~iii~~i*{iii

0. 12 iii}

{*

}ii*iii

!i!illi*{*iii~ ~i*

2 /1 /2 0 1 iiiiii{iii~iiii~iiiiil~

iii~iiiiiiiiii~iiiii...<.0 8

0. 2................~iii~iiiiiiiii, iiiiii~ii~liiii~~i!i~i Page 17 of 98

Table B-1. Sequoyah Nuclear Plant Di ffuser (Outfall 101) Discharge Concentrations of Chemicals Used to. Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 13, 2009 Date 01/02/2002 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01/07/2002 02/24/2002 02/25/2002 02/26/2002 02/27/2002 02/28/2002 03/01/2002 05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002 08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002 10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 10/11/2002 01/12/2003 01/13/2003 01/14/2003 01/1-5/2003 01/16/2003 01/17/2003 04/06/2003 04/07/2003 04/08/2003 04/09/2003 04/10/2003 04/11/2003 Towerbrom P C L -2122 PCL-401

ý.C L-3 63:

Cuprostat-mg/L mg/T.

mg/L.

mg/L.V PF TRC.

Phospha1ite Copolymer i.-DMIA.-D mg/L Azole

<0.0079 0,G23 0.02 0,006..

<0.0042 0.023 0.014 0.0124 0,P 02 4 0.014

. ~ ~

~ ~ ~ ~ ~ ~ ~ ~ ~..

< 0.0042

<0.0089 0,024 0.014

'0-006

< 0.004

< 0.004 0.023 *.

0.023 0.0143 0,12

23 0.023

.000-7

< 0.0041

J30.02

< 0.0041 0.024 0.008

< 0.0041

0. (124 0.008

!ii iii* i !iS * !!{

0.02 01 0.02 01 004 io 0.019 0.tS

.7 0.02 0..

14.

0,%i*

0.019

<0.0058

<0.0058 0.018 0.0092 0.018.

<0.010 o7

.0.055.ii l

.iiiiiiiiii 0.0o19 0007

-~~io~~::::i

<0.0061 0

5 0.0198 0.0152 0.4* 3 0.018 8.

< 0.0049 7 Iiiiii:{:i~~ : ::!::!l**~:<

i:i:ii!)!::*{iiiiiiii 0.0153

!iii.iii 0. 054ii iiii!

0.018 0009

-i*

iii~i

<0.0092 0.018 0.0124

0.

0055, 0.018 0009 0.0134 0.054 0.018 9 009

<0.0042 9.754 0.018

< 0.00 3 5

-iii*iiii,,iiiiiiii~,iiiiiii~il

ii}~~iiiiiii*:~~~~**

.0.00 0,054 0,019 00.......

<0.001 18 0.054 0.012

<0.0126 0.053 0.020 18 009

<0.0034 0i 0.

0.02018

<0.0034 0.0,6 0.010

<0.0073 5

<0.0189 0-5..

0.0219

<0.0117

18.

0.021

<0.0139 0.021 002.

0....

<0.0113 9 0.021 0018

<0.0073 0.022 Page 18 of 98

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-Febrtiary 13, 2009 Date 06/15/2003 06/16/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003 08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003 10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003 02/01/2004 02/02/2004 02/03/2004 02/04/2004 02/05/2004 02/06/2004 05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004 07/04/2004:.

07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004 Towerbrom PCL-22-2 PCL-401 mg/L ng/IL mg/L TRC" Phosphate Copolyimer

< 0.0045

< 0.0037 0.*45 0.020

<0.0048 0*041 0.014

<0.0048 0,041 0.014

< 0.0085 6,0--)s 0.020

< 0.0048

o.65s 0.020

<0.0050

<0.0050 0,058 0.020

<0.0051 0,057 0.020

<0.0084 0.05.7 0.020 0.0129 0 -0iiii 57 i

0.020 0.0153 0,057 0.020

<0.0043

0.05?

0.020

<0.0043

. 0,057.

0.020

<0.0090

.0.957.

0.020

<0.0106 00O$7 0.020 0.0181 0.022 0.0183

0026 0.024 0.0093

1 0.027 0.009

<0.0034

. 0.026:iii~**iiiiiiiii~

0.009

<0.0034 O.0V2*6.'.

0.009 0.0124 0.0l~iiliii!24.iiiiii~i 0.009

<0.0034 66:0i4 0.009 0.0105 0.0!*..

0.009

<0.0123 4..26 0.019

<0.0144

...("2.

0.014

<0.0146 0,02.0 0.013

<0.01227

.005 0.020

<0.0164

-002 0.0214

<0.01046 0,037 0.020 0.0227 0.057 0.020 0.016 0;06*.

0.021

<0.01 04 0,6SSiiiiiii

i~~~~~iiii 0.020 0.0217 0,0iiiiiiiii 57i!iiii!ii 0.019

<0.0085 0,0iiiiiiiiii57!iiiiii 0.020

<0.0077 0,058 0.020 0.0252

. 0.056 0.019 0.0223 0.057 00.019 0.0182 0,057,.

0.020 Cuprostat-PF mg/L Azole Page 19 of 98

Table B.-1. SequoyahNuclearl Ilant Diffuser (Ouitfall 1 01) Discharge Concentrations of

... Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling,

,*" M arch 12, 1998 -February 13, 2009

,Date,-..

n**ii*ii

'ii

_owerbrom ::iii i!i!

.PCL-401

ýJCL*K,63ii Cuprostat-PF H-1t30.'V Nalco ii*

%f~i, ".mg/L '..

L mgL*'::ii L`i:ii:

mg/L ing/L~iiiii 73551

., ii~iiiiiiiiiiiiiii~ i TRC.

Phosph u iii -C opolym er

-:IR:

i: -

A zoD at~~~~i a 11/08/2004 ii

<0.0192 0,04_7...iil~iiiiiii 0.030

((((i((((((i(((((({i i{!i((((iiii((iiig*

11/10/2004

<0.0149 0.016 0.041:::::::::::::::::::::

<0 0 49 i:***{:((i

{*{~iii

.0 6ii i*:s:*{a s*

11/11l/2004 i~i~iiii!iiiii{iiiiiiiiiii;

<0.0149 0i~{ii!((

i~ ~~~~~

.017 i:;!*

iiii:

02/07/2005 iiiiiiiiiiiiiiiiiiiiii!ii

<0.00 2 116~

!!!:i

iiiiiii 0.010

... 0.007~i}{i*iiii~iiii 02/08/2005

<0.0080 0,02.19~{*

*i..ii~ii 0.010

{ii!i;ii:;(({i{:((((

02/10/ 005..44..

0.......................

010iiiiii{iiiii~iii

. 0 02/11/2005 :::x

0. 155 0{iiiiii i -

iiiliii

.010 0.007:iiiiii::*i:::iiijii:::

0 6/ 0 5 / 2 0 0 5 0.0 0 63(({i

(({i~(((((((({

0 0 6

i *(({**!~ii((

i{*{*((((((

((((i(({i:@ (((({

06/06/2005 0.0043

.iii

.04ii iiiiiiiiii*iiiiiiiiii 06/07/2005 0.029103 i}ii*iii}i~ii

}i****~ii*~

iii}ii}

06/0812005 0.01295-:~~~~::::*~~~~~:::

0 6/0 9 /2 0 0 5 iiii~iiiiiii:*:i:ý 0 7/17/2005 iiii**i~i*?~iii!iiiiii 0.0 109

}?!))i?;)

0. 0...........**:::i~~~i:*:

* :::iiii:

~i[!:: !:::iii~::!

E*£*2i~ii[£i!522::iii

~i*7*~i~

~i~iiii!*

07/12/2005 iiiiiii!iiiii 0.0238

-X-:

iiiii!ili 0.009

s*:*~~s~g!**'iii:i~

. 1 6 7 /3 0 /2 0 0 5 iiiiiiiiiil~! iiiii!ii~iii..

0.0 16 3

.iiiiiiiiiiiii~

iiiiii~!!~ii

((i(({

(({!!ii~{i((*(({~((ii (((({i{ii 1 0 3 / 0 5 :::iX.

0.0 0 9

.:............i~i!i:'

0 0 1 i~~iii!iiiiii} iiiiiii}*lii*{ii

i::::iiiiii:g i:!:i:::i~~!.....................

07/021/2005 0 1042 O 'd 0.009

iii:ii::::i:{i:!

iii:!::::ii*!~iiiii:~~

.):iii:!:i

):i:i:*~*

10/315/2005 0,0125 ii iii 1 1/0 2 /2 0 0 5 !***ii!iiiii*i~i!ii~i}

!i~iiii*iiiiii 0.0 2 10

}::ii:i~

i:iiii::::::i:~~i~:

~~i*ii!*ii}iii~!}i~l 1l/18/2005 ~

~

~ ~

~ ~......

"20iiiiii:iiiiiii!:iiiii:i i

i 1 1/ 1 /2 0 5......................................

i..... 0.o 1 6 iiii~iiii~~i~~i~~i~*{ii~i~~i~~iiii~~ii{

i~i:::i:{~i~ii~.........i:i{

Page 20 of 98

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemnicals-Used to Control-Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling,

.March.12, 19.98-- February 13, 2009 te Sod ii Towerbror PCL-22 PCL-401 CL-363 Cuprostat-PF W130M Nalco I

Hyp*chlorite mg/L,

. mg/U mg/L m

ing/L mg/L mg/U 73551 m

mgL TRC

.PhTosphateý

.Copolymer DMD Azole Qut mg/L

....9.

T'RC

.EO/PO 12006 0.0055

'2006--

0.0068 0

/2006 0.0143 12006 0.0068

1. X

/2006 0.0267-0

'2006 0.0222

'2006 0.0188

'2006 0.0138

'2006 0.0120 12 0 0 6 0.0 2 8 8

'2006 0.0376 8

'2006 0.0187

!i~

? : ::

ii~

~i!:

8/07

/2 0 0 6~......

0. 3 6.....i3i~ii~iiiii iii~i]iiiiii!

9/07 0/07 0.0084 x.:::

1/07

.0.0103 1/07

~0.01640.1 i

wX.W.........

2/07

~~0.0305

......iiii::i~i 2/07

~~0.0241.....

0.0128....

0/07

0.0238 5/07 0.01758 1/07 0.01624 7/07 0.0143 3/08 0.0103 1/08

~~~0.01249K.....

0... 2 9...

0.

0 2

.6......

0....

0.015 1.....

7/09 0.01720 3/0 0.0 14 9:::::::::::::::::::::

0.0 154.

7/080.1 1/08 0.0082

.:i:ii:!i i:3:iiii:*::3 i:i:iiiiii:

iii::::* :::i*!3::::

1 /0 8ii:ii::i::i!::ii!:~

iiiiii i A

0 0 86::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::#:::::::::

Page 21 of 98

Table B-1. Sequoyah Nuclear Plant Diffuser (O/itfall 101) Discharge Concentrations of Chemicals:Usedto Control MicrobiologicallyInduced Corrosion Mollusks, During Toxicity.Test Sampling, March 12, 1998 -February 13, 2009 Date SAiumll Towerbrom PCL-222 PCL-401 IHypoch loritc mg/L" mg I mg/L

/L TRC

Phospbate:

Copolymer.:

TFC 02/08/09 0.0197 02/09/09-0.0237 02/10/09 0.0104 02/11/09 0.0155 02/12/09 0.0106 02/13/09 Cuprostat-PP mg/L*

Azole Nalco 73551 mg/L EO/PO 0.017 0.017 0.021 0.017 0.017 Page 22 of 98

Sequoyah Nuclear Plant Biomonitoning February 10-17, 2009 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 23 of 98

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 I,

Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc.

351 Depot Street.

Asheville, NC 28801 Phone:

828-350-9364 FedEx UPS Bus Client Other (specify): Sonic Deliveryý Delivered By (Circle One):

/,7 Facility Sampled: Sequoyah NP NPDES Number: TN0026450 General Conimen Chevy Williams Ben Mitchell Dissolved Metals Collecte Samples remained on ice through out sampling and transport to lab.

Collected By: Chevy Williams, Ben Mitchell Fax:

828-350-9368 I

Chevy Willi 02/09/09

)f ' 5 1 "sr I Sonic Delivery 02/09/09

//,

1 Sonic Delivery, 4

A 02/09/09 ETS 02/09/09, ssAET T

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc.

Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street.

FedEx UPS Bus-'

Client P.O. Number: N/A Asheville, NC Other (specify): Sonic Delivery,'.

~

~~~~~General Comment

)***-

Facility Sampled: Sequoyah NP 28801 ChevWliams:(

/

Chevy Williams:

NPDES Number: TN0026450, Phone:

828-350-9364 Ben Mitchell Collected By: Chevy Williams,.BeniMitchell Fax:

828-350-9368 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab.

Field Identification /

Grab/Comp.

Collection Date/Time Container Flow 52.q Sample Description Number &

cfs Rain Event?

Laboratory Use Volume (Mark as Appropriate)

Collected Date Time Yes if Yes, No Trace ETS Log Arrival By Time Appear-(EST) nchesance (EST, Inches Number Temp.

SQN-101-TOX Comp 02/10/09 -02/11/09 0915-2 (2.5gal) 2464 -

X I

0815 2494 Z

1i..

C_

eO 2471 VAI %r-t*

SQN-INT-TOX Comp 02/10/09 -02/11/09 0936-1 (2.5 gal)

NA X

\\

Sample Custody - Fill In From Top Down

-4 Lis L4,ýo Relinquished By (Signature):

Date/Time Received By (Signature):

Datbfime Chevy Williams

/ 7' 02/11/09 Sonic Delivery 02/11/09 Sonic Delivery 02/11/09 ETS 02/11/09 Is's5 51<1tss,3 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped'ovemighit on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until*.

,.f4ollowing Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc.

Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street.

FedEx UPS Bus

'Client P.O. Number:.N/A Asheville, NC Other (specify): Sore.pivery

~~~~~~General Commemi: S

i/

/

Facility Sampled: Sequoyah NP 28801 Geeraliom s:,

Chevy Williams

/

NPDES Number: TN0026450' Phone:

828-350-9364 Ben Mitchell Collected By: Chevy Wil.iaisI, Ben Mitchell Fax:

828-350-9368 Dissolved Metals Collected. Samples remained on ice through out sampling and transport to lab.

Field Identification /

Grab/Comp.

Collection Date/Time Container Flow

-C S 52,4(a Sample Description Number &

cfs Rain Event?

L ry Volume (Mark as Appropriate) a Collected Date Time

-Yes If Yes, No.

Trace ETS.

Arval By Tim.e A pear-

<A*

1

.(EST)

C

<-Inches

. Numb~er.*

Temp.

ance SQN-101-TOX Comp 02/12/09 -02/13/09 0936-2 (2.5gal) 2453-X

,4.

)

0836 2493 1 S:l. S AVG=

2470 --

. 0 SQN-INT-TOX Comp 02/12/09 -02/13/09 0842-1 (2.5 gal)

NA X

~

~0742-

-n Sample Custody - Fill In From Top Down 0o, Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time C0ilm 02/13/09 /

Sonic Delivery 02/13/09 Sonic Delivery*.,

0 02/13/09 ETS 02/13/09

\\ET -

-r5\\

~,,

Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: TVA,*:

.Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project 4:

24L County: Hamilton Treatment: Non-treated Outfall-101 Dilution preparation information:

Comments:

Dilution prep (%)

11.3 22.6 45.2 72,6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:

Test information:

Organism age:

t19. -1 14 01.)S 0 Lb Randomizing template:

iLLOW)

Date and times organisms

7. - O'- 0 6 0co0 Incubator number:

were born between:

Organism source:

ArO'*

't.tI Qf e -L-tf-OR Artemia lot number:

Lx& tog Transfer bowl information:

pH =

. Temperature =

°C Total drying time:

U_-kom 1, *LX{*

Date / Time in: oz*. 1l.o6 111()

Average transfer volume:

Date / Time out:uL.A.c.-d i\\

%C%

Oven temperature:

oc00" L Daily feeding and renewal information:

Day Date Morning Afternoon Test initiation, Control water Sample~numbers Analyst feeding feeding time renewal, or batch used used

time termination time I

O',L-Ik-c 01 oats 1q06 R'.- -ol 0 i 0 10i a6

4 G t At 02_L_

.-?,- OI-8

.S 12 00 Ocn--*

o,

\\

,*S 4

ca-.L.l

q.

Cý&Qo t"

w 10 %7-2-.

07.-"

10"0*

0407.t%. O 40*

(.-

5 1'1-IS-001 tr-sto Iq 01 o 1'

..- io--oF,

oo'

13.

oS -I 0 16 A

6 0Žo00 0,4o 10 It n~-lo~-Di &

O~tQoA3.4

'4. 01

~1

-~

7 cr7-- i-.."1 I r,2Jr,

-AL..J t.i, Control information:

Acceptance criteria Summary of test endpoints:

% Mortality:,

7.,

! 20%

7-day LC50

"> I or.

Average weight per initial.larvae:

o.0Z.l NOEC 10 07.

Average weight per surviving larvae:

OA2 1

> 0.25 mg/larvae LOEC 1

ChV.

> 1 o67, IC25 j

00%~7 Lage 27 of 98 a*

T *S~

Page 2 of 6 D I'a Species: Pimeph ales promelas Date:

Client: TVA / Sequoyah, Nuclear Plant - Non-treated Survival and Growth Da Day CONTROL 11.3%

22.6%

A B

C D

E F

G H

I J

K L

/t, ID 10 lb l IC

/rý 10

/6.)o

/0

/D 1

/0 t

1 t

o

/0 10 I0 t(C

/I

/

to to 2

1b It.)

Ito C) 10 O 1

( t 1

0 L 0 1 0_

30 ID 10 10 C1 Q) lb I

'o

/6 tob

/0 10 4

I D ID to 10 I

I(.)

lo

/0(to

/0"

()

/Q 5

J0 tOto f

113 1.

/0.

/0 (O

QD 10 t0 6

10

/0

)) /0 to

'0

/O

/0

/o

/0

/0 l/

I r3

)

/0 to 0o 1o fo 0

/0

0 A

= Pan weight (mg)

Tray color code:: 'Jtiou._ it U.13.41 iq.I 13.2.0 13.91 iq.li ISq,0 iq.s I

,'L0 Hq 9 [Q 13.15 Analyst: L.i Date-M B =" Pan + Larvae weight Analyst:

.nLr*

J

-t

'"{I Date:

03. "--I % -

C = Larvae weight (mg)

=A-B

.[

.g C.q5

.'A 6

[0 9

6.1 0

t.'to 6., Lt* I._N UKA~B

~,4 I

.2 l

.e3

.z

.I.

eb t.o t.'

.A

,. l 1

Weight per initial number of larvae (mg)

= C / Initial number of larvae A%

e2

'2 0

0 0

.5 0

6 110 A*

IV Average Percent weight per reduction initial from control

., l number of

(%)

larvae(mg) 0.-r kJage 28 of 98 Comment codes: c = clear, d = dead, fg=-fungus,k== killed, m = missing, sk =.sick, sm = unusually small, Ig unusuall.y arged&r-= decanted and returned, w = wounded.

Calcu.ations d

rie

Calculations and data reviewed:

Co mntnents- :"-'"".

(.

Species: Pimephales pjomelas.

Client: TVA / Sequoyah Nuclear Plant - Non-treated Page 3 of 6 Date:

9wth Data Survival and Gro~

Day 45.2%

72.6%

100%

_,,-_-M N

0 P

-Q R

S T

U V

W X

ID b

t 115 113

/b

/0

[1 t 0 10 to

'to:*

1 to L)

) /OIo*

to to

/0 to /0 1b*

/o 0

to IO

/o I1 C) to

'0 I )0 (Q0 1 1

3 I0

/1

/0 10

/0 (0

6 10 1

c

/1 to6 1'0 2

4_______

O (i 0

/0

/0 10 10.tO (O 0

/O (

/0~ I O

/0 i1 10 1 o 16o to to to) /0

/0

'0 5

to 10

-0

/0

.0 10

/0 I0

/1

/0

/t) 6 C)

I CC to

/0

/0~

r~

e 10

/a)

/0 to

/0 9

to

/o to

/0

'0 A

Pan weight (mg)

Tray color code::

5.9 1i.j"- I39T i'.1B Iq.35 q.kj u-)

I3.lq3q 9/i 13% '3.83 I1.3(o Analyst:

"35

+1 Date:

C7-tt__-__

B = Pan + Larvae weight Analyst:

fV1 ""

r.*"e

.3 g,I ~.3 z~* qb

.t oq*Il g I.*

Date:

C = Larvae weight (mg)

Weight per initial number

/,

of larvae (mg)

A

~

,ý 4

p C/Initial number of larvae ON

.P 7

1 11' 0

0" 0'.

Average Percent weight per reduction initial from control 0.13 7..

a.

ic

-0.

number of

(%)

larvae (mg)

Comment codes: c = clear, d = dead, fg fungus, k = killed,'m = missing; sk = sick,

.g.. = unusually large, d&r = decanted and returned, w = wounded.

sm = unusually small, Calculations andidata reviewed:

4,

age 29 of 98

E 3S.

-Page 4 of 6 Date:.

I..

Species: Pimephalespromelas.

Client: TVA / Sequoyah Nuclear Plant Non-treated

.~

N.,

.4,

'Survival and Growth Data Day 100% Intake Y

Z AA BB 0

1IO0 to

/0 to 2

/0

/0 C 0

10 3

10 (0

/o

/o 4

C

/0

/0 1e3 I0 5

IO to t0 to 6

/0

/6 (0

7

[C)

tb fl, A =Pan weight (mr Tray color code:: SbSI Analyst:._.

L.

Date:

61"/-10-09 B = Pan + Larvae weight (mag) 00 Analyst:

20,30* o*tAOý 4.3 jq,11 Date.

0;-- 1¶ -bc C = Larvae weight (mg)

=A-B Weight per Initial number of larvae (mg)

I

ý 4

= C / Initial number of larvae

\\9 Average Percent weight per reduction initial from control 0o l

3.scl.

number of

(%)

larvae (mg) tage 30 of 98

.omment codes: c clear, d = dead, fg = fungus, k= killed, m missing, sk = sick, sm. unusually small, g = unusually large, d&r decanted and returned, w = wounded.

Calculations and data revieNe d:

Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated February 10-17, 2009 0

S4D Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.

Project number:

5246 Reveivwed by:

Not for Compliance Assessment, Internal Laboratory QC COnetrationa )

Replicate Iitial camber of P9 aclumber of A - Pan ight B-Pa.+Larvae Larvaeweight (sg)

WdghetlSareviAg MMean eighlt Coeieeatohvariaion Weigbts Initial omber Mslansurvival Meancigtight/

rialt of

[Percentreducio froom larvat tr-a (ag) weghlt (ag)

-A-B samberflarvae(wll Sarvyilagnamberou

5.

n of larvae (mg)

I%)

Initial aumber of variatioe Im.-tp, coatrul (%)

A to to 14.32 20.76 6.44 0.644 0.644 Control B

10 o

14.28 21.06 6.78 0.678 0.621 82 0.678 100.0 0.621 8.2 Not applicable C

10 10 13.41 19.38 5.97 0.597 0.597 D

t0 0

14.05 19.68 5.63 0.563 0.563 E

10 10 13.20 19.50 6.30 0.630 0.630 11.3%

F 10 10 13.91 20.19 6.28 0.628 0.628 4.7

.3.0 G

10 t0 14.17 20.33 6.16 0.616 0.63 0.616 100.6 H

10 l0 15.90 22.73 6.83 0.683

. 0.683 I

10 t0 14.51 21.21 6.70 0.670 0.670 J

10 10 14.20 20.99 6.79 69

.676

7.

0.679 226%

0 900.0 0.676 7.9

-8.9 K

10 10 14.49 20.61 6.12 0.612 0.612 L

10 10 13.75 21.18 7.43 0.743 0.743 M

10 10 15.59 21.73 6.14 0.614 0.614 45.2%

N.

10 10 14.57 20.76 6.19 0.619 0.627 7.4 0.619 97.5 0.613 11-2 0

10 10 13.92 20.85 6.93 0.693 0.693 P

10 9

14.78 20.03 5.25 0.583 0.525 Q

10 10 14.35 20.12 5.77 0.577 0.577 72.6%

R l0 10 14.91 20.13 5.22 0.522 0.584 9.6 0.522 100.0 0.584 9.6 5.9 S

10 10 13.57 20.15 6.58 0.658 0.658 1 0 tO 13.86 19.65 5.79 0.579 0.579______________

U 10 10 13.99 20.04 6.05 0.605 0.605 100%

V 10

I0 13.55 20.97 7.42 0.742 0.619 14.2 0.742 100.0 0.619 14.2 0.3 W

10 10 13.83 19.76 5.93 0.593 0.593 X

t0 1

0.

14.36 19.70 5.34 0.534 1

0.534 Y

10 1

0 14.08 20.30 6.22 0.622 0.622 100% Intake z

3.90 2002 6.12 0.612 0.612 1.6 0612 97.5 0.597 5.1 3.9 AA

!0 10 12.74 18.73 5.99 0.599 0.599 BB 10 9

14.39 19.92 5.53 0.614 0.553 outfall 101:

Dunnett's MSD value:

0.1030 PMSD:

16.6 Intake:

Dunnett's MSD value:

c*'

5.

0.0575T-PMSD:

9.3 MSD -

Minimum Significant Difference PMSD -

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity lest.

Lower PMSD bound determined by USEPA (10th percentile) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the loth and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboialbry Variability. Study (USEPA, 2001a; USEPA. 2001b).

USEPA. 2001 a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated EuivironmentaI Testing Solutions, Inc.

February 10-17, 2009 Statistical Analyses Start Date:

2/10/2009 End Date:

2/17,/2009 j, Sample Date: -

Test ID:

PpFRCI Lab ID:

ETS-En Protocol:

FWCHM Larval Fish Growth and Survival Test-7 Day Growth R

Sample ED:

vir. Testing Sol.

Sample Type:

R-EPA-821-R-02-013 Test Species:

TVA / Sequoyah Nuclear Plant, Outfall 101 DMR-Discharge Monitorling Report PP-Pimephales promelas Comments:

Conc-%

1 2

3 4

D-Control

`0.6440 0.6780 0.5970

.0.5630 11:3

.0.6300 0.6280 0.6160 0.6830 22'6 0.6700 0.6790 0.6120 0.7430 45.i2 0.6140 0.6190 0.6930 0.5250 72.6

. 0.5770 0.5220 0.6580 0.5790 100 0.6050 0.7420 0.5930 0.5340 Transform: Untransfoifned I-Tailed Isotonic Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Mean N-Mean D*Control 0.6205 1.0000 0.6205 0.5630 0.6780 8.174 4

0.6453 1.0000 11.3 0.6393 1.0302 0.6393 0.6160.

0.6830 4.664 4

-0.439 2.410 0.1030_-

0.6453 1.0000 22.6 0.6760 1.0894 0.6760 0.6120 0.7430 7.934 4

-1.298 2.410 0.1030 0.6453 1.0000 45.2 0.6128-0.9875 0.6128 0.5250 0.6930 11.220 4

0.181 2.410 0.1030 0.6128 0.9496 72.6 0.5840 0.9412 0.5840 0.5220 0.6580 9.582 4

0.854 2.410 0.1030 0.6013 0.9318

.100

!.0.6185 0.9968 0.6185 0.5340 0.7420 14.226 4

0.047 2.410 0.1030 0.6013 0.9318 Auxiliary Tests "

Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95722824 0.884 0.42652272

-0.0353657 Bartlett's Test indicates equal variances (p = 0.69) 3.06386232 15.0862722 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett'sTest 100

>100 1

0.10304414.

0.1660663 0.00375797 0.00365631 0.43092009 5, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point SD 95% CL(Exp)

Skew IC05 45.035

{-<

ICI0

>100 IC15

>100 IC20

>100 IC25

>100 IC40

>100 IC50

>100 sqn 101I.02-1O-O9data

vs S

S 0

TVA / Sequoyah Nuclear Plant, Intake - Non-treated February 10-17, 2009 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Stairt Date:

2/10/2009 Test ID:

PpFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Intake End Date:. :,2/17/2009 Lab 11D:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report' Samlle Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comment~i:*

Conc-%ý%",

1 2

3 4

D-Control 0.6440 0.6780 0.5970 0.5630 100 0.6220 0.6120 0.5990 0.5530 Transform: Untransformed I-Tailed Isotonic Conc-%:

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Mean N-Meani D-Control 0.6205 1.0000 0.6205 0.5630 0.6780 8.174 4

.0.6205 1.0000 100 0.5965 0.9613 0.5965 0.5530 0.6220

-5.112 4

0.811 1.943 0.0575 0.5965 0.9613 Auxiliary Tests...-

Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95853066 0.749

-0.2030801

-0.8613815 F-Test'indicates equal variances (p = 0.43) 2.76694155 47.4672279 Hypothesis.Test (ltail, 0.05)

MSDu MSDp MSB MSE

. F:Prob df Homoscedastic tTest indicates no significant differences 0.05749647 0.09266151 0.001152 0.001751 0.44827035 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point SD 95% CL(Exp)

Skew IC05 IC00 IC 15 IC20 IC25 IC40 IC50

>100

.~ -~-..

sqn 101_02-10-09data

ET SEnvironmental Testing Solution s, In.c.

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated February 10-17, 2009 Pimephales promelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number:

5246.

Reviewed by:

Concentration Parameter Da 0 DaI Da 2 Da 3 Da 4 Day 5 Da 6

________Initial IFinal Initial Fnl Initial Fnl Initial Final Initial Fnl Initial IFinal Initial Final F i F i naF il pH (SU).8.011 7.78 7.82 7.1 7.70 7.7 7.691 7.74 7.72 7.0 7.801 7.69 7.74 7.44

7.

7 0

7.7.7.

DO (nig[L) 7.51 7.6 7.8 7.7 7.75 7.6 7.4 7.57.

8.21 7.4

. 7.6 6.6 Conductivity (pmhos/cm) 325 5

321 30 345 Control Alkalinity (mg/L CaC0 3) 62 63 Hardness (mg/L CaC0 3) 96§90

_Temperature

('C) 24.6 24.3 246 245 24.8 24.2 24.6 24.3 24.7 246 24.8 24.6 24.7 24.7 pH (SU) 7.88 779 7.80 7.80 7.86 7.70 7.81 7.72 7.86 7.57 7.88 7.71 7.83 7.30 1DO (mg/L)

7.

75 7.7 7.8 7.9 7.5 77 73 79 7.7 8.4 7.4 7.7 6.6 11.3%

Conductivity (pmhos/cm) 302 312 305 300 304 3

Temperature 24.7 24.1 24.7 24.5 24.8 24.4 24.7 24.5 24 7 24.6 24.9 24.6 24.7 24.7 pH (SU) 7.89 778 7.87 7.79 7.89 7.71 7.81 7.70 787 7.57 7.88 7.74 7.83 7.38 22.6%

DO (m]g/L)

.78 75 7.7 7.8 7.9 7.6 78 73 79 7.7 8.4 7.5 7.7 6.2 Conductivity (pmhos/cm) 290 00 293 21 290 28Y 300 Temperatur (*C) 24.7 24.4 24.7 24.6 24.8 24.4 24.7 24.7 24.8 24.3 24.9 24.5 24.7 24.5 pH (SU) 7.89 781 789 779 790 766 781 773 788 757 788 7.77 7.84 738 45.2%

DO (mg/L) 79 76 77 75 8.0 75 79 74 78 77 85 7.5 7.7 63 Conductivity (lpmhos/cm) 264 275 270 268 268 262 275 Temperature( (C) 247 242 247 244 24.8 245 24.7 244 24.8 24.5 24.4 24.8 245 pH (SU) 92 781 792 783 7.92 775 7.81 777 789 762 7

.. 7.76 7.85 739 72.6%

DO (agL) 80 76 78 7.5 8.0 75 8.0 75 80 77

.5 7.5 7.7 63 Conductivity (pnihos/cm) 237 245 241 240 233 244

_______Temperature

(.C):.

24.7 24.2 24.7 24.4 24.9 24.5 24.7 24.4 24.8 44 24.9

'24.6 2484.

5:

24.9 4.7 6

24.

24466 pH (SU),

7.94 7,79--_

7.941 7.831 7.931 7.741 7.81 7.78 7.90 7.61 7.881 7.791 7.85 7.40 DO (mg/L) 8.1 7.4 7.7 7.7 8.1 7.4 8.0 7.4 7.8 7.6 8.5 7.6 7.8 6.3 Conductivity (mhosm) 209216 211 218 208 20 100%

Alkalinity (mg/L CaC03) 74M 70 75 Hardness (mg/L CaCO3) 86 82 86 Total Residual Chlorine (mg/L)

<0.10

<0.10 Temperature ('C) 24.7 24.2 24.7 24.2 24.9 4.3 24.71

24.

24.9 24.3 24.9 24.3 24.8 H (SU) 7.94 7.83 7.95 7.84 7.94 7.74 7.85 7.75 7.90 77.86 7.40 DO (mg/b) 8.2 7.6

.7.7 7.7 8.2 7.4 8.0 7.3 7.9 7.61 8.51 7.6 7.7 6.4 Conductivity (Iunhos/cm) 206 208 212 100% Intake Alkalinity (mig/L CaCO3) 74 72 7

Hardness (mg/L CaCO 3) 92 82 Total Residual Chlorine (mg/L)

<0.10

<0

_ TemperatureC' 24.71 24.21 24.8 24.21 24.7 24.5 24.7 24.5 24.8 24.4 24.9 24.5024.8 24.4 p3;

V.

Species: Pimephales'p'romelas.

Client: TVA /Sequo6Zahli uclear Plant - Non-.treated Daily Chemistry:.AT P.age 5 of6 jate I

I Day 0

llh&..-

I M.L.r-~

i 1~

0,

1 b,

2&lI CInentratn I P ar nalyst e*-"

/*

Concentration.

T arametersý,

Nw CONTROL pH (S.U)-

S.OI

1 I' ThA L$L4?

- 6.1 I

I DO (mg/L)

0.S COnductivity (4rmhoslcm)

Alkalinity (mg CaCO 3iL)

Hardness (mg CaCO3/L)

?)

o'o

~

II U

q Y

I Temperature ('C) 1,k.6 II L'4.L.

I pH (S.U.)

7-4,6 11 j,~~ I q

1 4.RD117 L_*

gb 1 -4 6 1 1-11.3%

DO (mg/L)

Conductivity3o t

q.:* *F r~

~.

Temperature ("Q I.

LJ L

I I

pH (S.U.)

II7 9 1

-4s.

I 1

_0 L

4, eg r4 q

ý93 14 -

22.6%

i I

[

I*

=

DO (mg/L)

Conductivity (i*mhos/cm) 290 i

Temperature (°C)

I)A1.

'I 4

I ~

DH (S.U.)

II ~

I Th~ II 7~~q I ~*Ao II 1.6$

IiA..,

a J

I 3AlA 11F)-41 1

, 5 45.2%

DO (me/L)

Conductivity (g.mhos /cm)

-ZIA -1 F k T

31.

11 Temperature ("C) 4 4-pH (S.U.)

7~*? TY 1 7 1 772 11~

I314 4-a 1ý i-1

-is, 72.6%

DO (mgCL)

-Conductivity (iimho /cm) 9..

I 98 )

1 Temperature (QC)

_1q.14 1

N.

1ý 1-1, 4

4.

pH (S.U.)

1q. 1 4,7Iq I -7,,q, 100%

DO (mg/L)

.1 II Conductivity (pmhos/c m)

Alkalinity (mg CaCO31/L)

-1

~

Hardness (mg CaCO3fL) g6 TR chlorine (mg/L)

Z 6,.10C

'T q-taj-iý 10 4 0. 113 I

I I

Temperature (0C) n 1

I MH (S.U.)

.100% intake DO (ri/L).

Ab~a Zoo Conductivity (ymhos/cm)..

Alkalinity (mg CaCOýIL)

Hardness.

(rag CaCO3/L)_

TR chlorine (mg/L)

-ILA CO. 10

a. JO 24.7 l2q.2t

'2Q4, 14.2 1{IV i Temperature MC)

II L'l-s I

I______"__III_"

I4 I

I I

Initial II Final I

Initial II Finali I age 35 of 98 4* PVN

...- Tea %..Ca COO_64M10k3 (..JvtqcE

i 01 r

0

.~7YA*

I Pag

..r Sag t

.Species: Pimephaiespromelas Client:' TVA,/.9SUoyah Nuclear Plant,- Non-treated Date- -

oZ.*t e 6 of 6

-A Concen-tration CONTROL

~*, 1 D ay: °' _'" <::*. <.

I

Day I:.

-4 I.

5 I

=

Analzst in Parameter m

~'...

J fJ w

oH (S.U.)

II 4-.Infl 171S'4)

II

.-i~-c~

I

-~-iu II IIr..,.~

n

LL

~t-~I It

'~'

I m

DO (mc/L)

Conductivity (p.mhos/cm)

Alkalinity (mg CaCO 3IL)

Hardness (Mg CACO 3/L) 37-1

1.

(A 3LIG

~&L~

,'.J.1 tl m

P Hi m

Temperature (0C) pH(S.U.)

1.P

-.7D _ý 1,3 11.3%

DO(mg/**

1..

Conductivity 3oo 3O'7f mprhos/cm) 3r312 Temperature ('C) v-11 A.S1,

1A.

1

.(

2I.1 2.1 22.6%

DO (mg/L)

.4.

E'(~2 Conductivity 300 (pimhos/cm)

Temnperature(°C) 2(

Q1 q'i

-q*

"t'.

i".

pH (S.U.)

a.

TV 2-

'-.8.

45.2%

DO (mg/L)

1.

I..-

Conductivity 21L5 Temperature (T) 2 1.._ L 2.....4*S PH________

T7 1

.bj~

1 72.6%

DO (mg/L)

Conductivity

-2 C)a33 Temperature (TC) vi

.1

'4 zq.

2 1

'41 100%

100%

Intake 36 of 98 MH (S.U.)

ma' 1.18 ~Li~cto It Tht~ol I%q I1ýI1.

LŽQ DO (mc/L) 0 Conductivity (ijmhos/an)

Alkalinity (mg CaC0 3/L)

Hardness (mg CaCO3/L)

TR Chlorine (mgIl)

Tern eratu m( 0C1..

& 0.10oC 21.1L1 I

oH (S.U.)

Ii~;~ II m~JxLiLIsV LI

~~7~

II-II I

II m...

nn (jumhos/cm)-

1 7.2

,,ý,-

Alkalinity (mag CaCO3/L).

Hardness

TR chloriiie (/rig/L)

0.I Temperature (°)

qs 2q.?*

"-11t l3~~

21.45 1

?14.cr 24M9~1 Final I

Initial t

Final Initial U Final I

Initial i Final I

iT

'4..

Page 1 of 7 7,.

'-~W'

'V..

."Chronic Whole Effluent Toxicity Test (EPA-821-R-ý02;013 Method 1002.0) lient: :

Species: Ceriodaphnia dubiaý,'

Chiet:

I County: Hamilton Facility: Seguoyah:Nuclear Plant.

Treatment:.,Non-treated

..NPDES#:TN 0026450.-

<ýOutfall: 101 Project #:

5 2S-,1 170 Dilution preparation information:

Comments:

Dilution prep (%)

11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 565 1130 1815 2500 Diluent volume (mL) 2217.5 1935 1370 685 0

Total volume (m.L) 2500 2500 2500 2500 2500 Test organism source information:

Test information:

Organism age:

< 24-hours old Randomizing template color:

a a P-0 Date, and times organisms were born 010. Olt m? o Tu.4~$

Incubator number and shelf between:

location:

z.A Culture board:

n-a'b-o'% A Replicate number:

1 12 13 14

-S 6

7 8

9 10 YWT batch:

0 1

0 Culture board cup number:

I

L**1* t, I

I*

l Transfer bowl information:

pH =. 1-A.1 SU Temperature =

"1 *

'C Selenastrum batch:

U5/--ý-O Daily renewal information:

Page 37 of 98 Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0__

02.-10-0 022)

OtL &Al

.1 Lr0Fý otc.0ct 4a-?-

13

~

Ol C5.01-01 &~

Ockoz 0 1-4 Is.

o- -

oz,-*t

.**ot t

4 in, IA"5

+'z--o oIpctoS *Qo ALO 6

lcrz-lt.-04ý CA -----

7ý Control information.'.:

1

.Ace

%:0fMale Ad~lts:

o7..

%.Adults having 3rd Brbods:.ý",

%,Mortality:

eptance cnteria 2(,:: Summary of-test idp'""ints 27-day Lv.K4'*

H 477 '

L 0 KtC 4o:fpigfml' o %.li[

JL 2

ý IMean Offspring/FetiM&>~:>j 3~.P] ~'.i I ŽŽ is:c

%-CV:Zý:-

LI; 7~ z

&37.

b I -

I

E T Species: Ceriodaphnia dubia Client,:;.Seq uoyah Nuclear Plant - No n-treated C IN1T RQL--.. I,.7:

Surv, Page 2 of 7 Date:

02i0-pq ival and Reproduct'ion Data

.,Replicatenumber 11 2,"

3 r7.

5 7

8 9

10

""*"'Aduilt ff6io~aliiý...\\._...__-L"

"*'k.

., '-<~...

2 1-Young produced.

C___

C Adult mortality I

i U-U U.

U 2

Youngproduced I J

01 I0 ID o 1o l 0 j 0

. 0 Adult mortality ITI L.-

I

[

_ I L-L.

t.-.

4 Young produced LQ-1

,-t o ISI Llko Adult mortality k.[

%-- [

\\-L

-j j

LJL Young produced

'A

[Adult mortality 5,Young produced

'i1'2AI'\\

is1 1

Adult mortality [A.. -

I' 7

Young produced Total young produced

\\

V 3 2 C.

3 k 3b 3

Final Adult Mortality X for 3"d Broods 52--

1-Note: Adult mortality (L live, D dead)

Concentration:

j

% Mortality:

I 07.

Mean Offspring/Female:

,1.,0 CONC: 11.3%

Survival and Reproduction Data Replicate number Day,_

1 2

3

[

4 5

6 7

8 9

10 1

Young produced 0

Adult mortality U.

L L

2 Youngproduced

_l

_0!

Adult mortality 4

Young produced

'A

_k 71 S,

Adult mortality L...,__

5 Young produpced

__L I_

_I Adult m ortality

. Yoi u gOprodu-ced

__r _

Fi a Adult mortality

~jYoung rodducedj

,TotalFyoung pro duced.,&

p 2

ýFinal AdultMraity~

.>..'~

.~

7 C-U k*

LNULC ti~UiLt WU I1OHLy;' tL:.,ý LýV C, U.

1 J

uc )

G) 7.70.

I IPage 38 of 98 I Mean Offspring/Female:

.32 *,.

% Reduction from Control:

- I.' 7.

3

-S nt!Wtj w

Page 3 of 7 4.i

.x; 7; 'Y : '","

"..'Species: -SCeriodaphnia dubia

~ lient: Seq uoy~ah. Nuclear Plant - Non-treated Date: "ti'oq Ca IS..

duction Data CONC:22.6%

Survival and Repro

__[ Replicate number 4

ý,Day l

2 3

4 5

6 7

8 9

10 V

"":::Y u'g rded::

S' S; -

1

(

I S" [

dto t

".You p

uo o

o I

p. r d u ed 7Adult mortality

-L ' J

k.

I L_.]

k-I k..

II..

I I.

2 Young produced 0

O l

l o

Adult mortality

-t-

II

%- I-3 Young produced I 0 I I_0101 0

Q 01 0

a o o I

Fnl Adult-mortalit y

U

L

..- [

\\.-.

"_.. J

\\... -

k.

I

4.

Young produced L_

Lk

-A Note:

Adult mortality D.

[

f 6

Youngproduced 7

Conn Adult mortality

\\.'- I-

\\-

-I'-I

\\-

Adul motait Motliy_"

0 SYoung producedO Total young produced 3

X u5 3 35n 35 31 3,S D

Final Adult Mortality l

Note: Adult mortality (L =live, D dead)

Concentration:

% Mortality:0 I

IMean Offsprin/Ffemnale:

3-6-S

% Reduction from Control:

-4.1 7.

CONc: 45.2%

Survival and Reproduction Data T _____

Replicate number Day 1

2 3

4 5

6 7

8 9

10 1

Aoundproduced I

o.

oImortality 2

Young produced 1 0 c)

[Adultmortality I -

I I l I L LII ÷-

3 Youngproduced I I

__l__ 1I cI Io 0

1

[ Adultmortality I LA -

L-II t

I I

I I.I 4

Young produced k

LI

[Adult mortality I

'-I.

4_.I_'.---H-1

_I Yohhg producid TI I

'L t 1

,1

[.Young.produced I t"

' 1

'[

1

' I

[.u.

.mo.tality_

JuL]--)(ungiroduce+/-.

-33 3-d

-TotaI-y'Oung~pr-odu'cd-z,

3.

3$

Final AdultMortalityý:

NZ~L L

I' Ii ii rn:.

~iage 39 of 9~8 Not&dult mo~rtality "L--" livp.,D dead)-

1~i Concentratzon:Ž'::,~

"/o Mortality:

-~

I I

1 1 Mean Offspring/Female:

1.3. 2.

I I % Reduction from Control: I-I.A.

I

ET Species: Ceriodaphnia dubia Client: Seqi0oyah Nuclear Plant - Non-treated

...ON: 72.6%

Surv Page 4,of 7 bate:

02I "0..

4 ival and Reproduction Data L.

"Replicate number Day'

.1

[

2 3

.4 5

6 7

8 9

10 T. Y

..i.. ug. produced-:

C>' -.0 :

0 "(

(

0... C...( *

"il i ° A dult. m ortality,,*:, : -

2

Y Noung produced r

J_

]

{

Adult mortalit y

L.---

3 Young produced '>

C)

C a

Adult mortality LI..,

L_..

I.....

4 Young produced LDJ 1.1 1

1

[

4 Young produced IA I

Toa young produced I*'

5"

'A1 iL L.... I,

_'-* I*:

Adul t mortality

[

. I L._.[_

C 6 [Young produced ["I'i~I'tL'~

~I'

~Ii Adult mortality

~i-[~I~

s

'1

'1'

~

7[

Young produced Total young produced 3

3 3.3 Final Adult Mortality

\\.

\\..

I

' tL.-.L. ~

L 2

Note: Adult mortality (L live, D dead)

Concentration:

% Mortality:

67.

Mean Offspring/Female:

35. 6o

% Reduction from Control:

/t.

5"/

CONC: 100%

Survival and Reproduction Data Replicate number Day 1

2 3

4 5

6 17 8

9 10 I

Young produced

("

)

o3

. [ Adult mortalit "L-L_-

L._

I..

2

[Young produced.I o

d.-

[(j C I 0 1 I Adult mortality LI 71j JL-j Lt* -

3 Young produced 1I

o.

I I.

0 1 o Adult mortality I..

I...

L L..-.

Youngproduced 6

S 6

I Adult mortality l*

l

%.I V _

I I

SYoung produced Iq vT4___I

'AIA

-S~t I

-~L -L~i' Adultzkortalit~y I--ý6_j -

.. J U

-7' "Youngproduced t

STotal young'produced FinaleAdulftMortalit U.

.Z

\\.

'C C,

,2T v

-9 p

44

' Note: Adult mortality..(L= live, D dead) 4,'

Cnenhtra-tio~n:.~ '

... % M bitality-`-':..."

O,

Mean Offspring/Female:

3f..9

% Reduction from Control:

- I'S 3 Iage 40 of 98

K.'

Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant'- Non-treated Page 5 of 7 Date:

OZ-. 10. 0a duction Data:

_. CONTROL: -2 Survival and Repro*

Replicate number Day -

._l 2

3 4

5 1

6 1

7 9

1 10 Yung Rroduce'dj~l c>

cŽ 0L Adultmortality"..

2 Young produced I0 I

01 o ! o I l 1

0 l Adult mortality C

I I

I L

L-3 Young produced Cj) oi Lo I o Lo I

Lo Adult mortality.[

4 L

--4

-- J 4

Young produced [

s.

k..

Adult mortality [

'- I

'-I I

I

-I-5 }Young produced iilmraiy t

[

Afv[

l1'-kLL 61 Young produced It LIA I1 l-1 ILA4

\\,5 L4 isj{

Adult mortality k-L 7J Young produced

.Total young produced

62.

\\

33 51 3,

3-3

.50 Final Adult Mortality k

1 ?

i..

. L...

z Note: Adult mortality (L = live, D = dead)

I Concentration:

% Mortality:

c1. I Mean Offspring/Female:

CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1

2 3

4 5

6 7

8 9

10 Young produced C) 2 Young produced 0y 0

C)

I Adult mortality L L..

L L

.,tL..

3 {Young produced [..D..Q cL...o.

o o

ilC)

Adult mortality I

U [

I_.

1 0

4 Young produced [

Adult mortality

___1_Ad ult mo rtaltyk.

.Yoqungproduced AIM

.1 R 19 :

7_1 1-1 19 I

4.

I.-=-----I.

-4.

4.

I.

-+

'Aduilt hinoit~litv A_

_-YngPr~dced Final Adult Mortality N.te: Adult mortality (L= live, D dead)

-f--I I

I

[

~

'31

'-lb

,3S 3~4 31--

-M I.

"incentration:

. *,.:, =;.-I

%Mortality' 07.

Mean Offspring/Female:

1

% Reduction from Control:

- i2.16.7 Page 41 of 98

0

-0 S

Environmental TesUng Solutions, Inc.

Control 14 TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated February 10-16, 2009 Verification of Ceriodaphnia Reproduction Totals 72.6%

Re licate number Day

,1 2.,3 4

5 6

7 8

9 10 1

0--

0 0

2 0

0 0

3 0:

0

0.

0 0

4 7.'

5 5

4 6

4 4

5 48 5

A 1 12 I1 12 12 14 11 13 13 12 121

6

.16 14 15 14 16 17 14 14 16 15 151

.7al Total.

34 31 31 30 34 35 29 31 33 32 320 ii Day "Replicate number total D 1U.21 3

4 5

6 7

8 9

10 1r 0

0.

0 0

0 2

0.

'0 0

0 0

3

.0"

-0.;

0 0

0 0'

0 4

6

.4

.5 6

6 4

4 4

4 5

48 5

12

12 13 11 14 13 12 12 11 12 122 6

.15 17 17 14 15 17 14 16 16 15 156

7 Total 33 33 35 31 35 34 30 32 31 32 326 22.6%

Replicate number Day

-r..

Total i

1 T.27 3

4 5

6 7

8 19 1

1

01 0

0 0

2 0

0 0

-3 0

0 0

0 1 0 0

0 4

4

'6 4

5 5

5 4

6 4

5 48 5

15 11 12 12 13

.12 12 14 12 13 126 6

16.

14 17 15 17 18 15 15 17 17 161 Total 35 31 33 32 35 35 31 35 33 35 335 45.2%

Re licate number Total Day

-1 2

3 4

5 6

7 8

9 10 1

0 0

0 2

.0.

0 0

3 0

0 0

4 7

5 4

4 6

5 5

5 51 5

12 11 14 12 12 13 12

.12 12 13 123 6

16 19 15 16 18 18 117 16 18 15 168

.7 "Total ]!35. 1.35 1331 32 136 135 135 133 135 133 342 Replicate number Day T

2 3

4 T

j-Total a

1 -

2 3

4 5

6 1 7

.8-9 -

10' 1

0 0

0 F0ý 0

, 0 0

2 0

0 0

3 0

0 0

0 O' 0 0

0 0

4 4

6 5

5 4

7 4

6:

6 -

5 52 5

13 13 14 12 12 1513 12 13 13 130 6

18 15 18 17 19 16 19 19 17 16 174 Total 35 34 37 34 35 38 36 37 36 34 356 100%

Replicate number Total Day 1

2 3

4 5

6 7 18.

9.

1 T10 1

0 0

0.

0'

.0 "0

0

.2 0

0 0

3 0

0 0

0 01 0 0

0 0

4 6

5 5

7 5

5 6

6 55 5

12 13 14 14 12 15 12 12 13 14 131 6

19 17 19 19 19 17 19 18 20 16 183 mnmmiEIE Ell Total 37 34 39 38 36 39 36 35 39 1 36 369 Control-2 Replicate number Day 1

2 3

4 5

6 7 18 9

Total 1

0 0

0 a

0 0

2

.0 0

0 0

0.

0 0

3 0

0 0

.0 0

0 0

.0 0

4 5

4 4

4 5

5 3'

':4 43 5

11 13 13 12 14 12 12 IL 14 11:

123 6

16 14 14 17 14 15 16 16 14

.15 151 Total 32 331 31 33 33 31 33 32 31 30 317 100% Intake

Replicate number.

Total Day 1

2 3

4 5

6 7

8 -r9. 9-'10 Tl, 1

0 0

0 L

2 0

0 0

0 0Q 0

0 3

0 00 0

0 0

O" 0

000 0

4 4

4 5

4 6

5 5

4 5

4 46 5

13 13 12 10 13 14 13 11 13 12 124 6

17 20 18 19 18 21 17 19 19 19 187 Total 34 37 35 33 37 40 35 34 37 35 357

' i

0%

Environmental Teesting Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101;- Non-treated February 10-16, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses

Project numiber: .

Reveiwed by:,

jj 5246 Concentration Replicate number Survival Average reproduction Co7ficient of Percent reduction from

(%)

1

2.

3 4

5 6

7 8

9 10

(%)

(offspring/femkle).

variation (%)

pooled controls (°)

Control - 1 34

.31 31 30 34 35 29 31 33 32 100 32.06 6.1 Not applicable 11.3%

33' 33 35' 31 35 34 30 32 31 32 100 32.6 5.3

-1.9 22.6% K' 35,".

-31 f 33 32 35 35 31 35 33 35 100 33.5-51

-4.7 45.2%

" 35 35 33 32 36

35.

35 33 35 33 100 34.2 3.8

-6.9 72.6%

35 +

34,

37 34 35 38 36 37 36 34 100 35.6 4.0

-11.3 100%

37; 34 39 38 36 39 36 35 39 36 100 36.9 4.9

-15.3 Control - 2:'

32 331 31 33 33 31 33 32 31 30 100

31.

3.3 Not applicable 100% Intake 34 37 35 33 37 40 35 34 37 35 100 3A.7 5.8

"-12.6 Outfall 101:

Dunnett's MSD value:.

PMSD:

Intake:

Dunnett's MSD'value:

PMSD:

"0 E1:703' 5.3 "1.'269+"

4.0 MSD =

Minimum Significant Difference PMSD =

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10i percentile) = 13%.

Upper PMSD bound determined by USEPA (9 0'b percentile) 47%,

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from:EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2000. Understanding and'Acdounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003.-US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82 1-13-01-004 and EPA-821-B-01-005. US Environmental Protection Agencyi'Cincinnati, OH.

SEnvlronmental Testing Solutlons, In TVA I Sequoyah Nuclear Plant, Outfall 101 - Non-treated February 10-16, 2009 Statistical Analyses C.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:

-2/10/2009 Test ID:

CdFRCR Sample ID:

TVA / Sequoyah Nule6r Plant, Outfall 101.-..',.

End Date:

2/17/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge MonitoRng pR t.

Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubia Comments:

Conc-%.

1 2

3 4

5 6

7 8

9

.10 D-Control

.34.000*

31.000 31.000 30.000 34.000 35.000 29.000 31.000 33.000 32.000-.

113

[33.000 33.000 35.000 31.000 35.000 34.000 30.000 32.000 31.000 32.000

'22.6

." 35.0002.

31.000 33.000 32.000 35.000 35.000 31.000 35.000 33,000 35.000 45.2. v 35.000, 35.000 33.000 32.000 36.000 35.000 35.000 33.000 35.000 33.000

72.6

.. -35.000, 34.000 37.000 34.000 35.000 38.000 36.000 37.000 36.000 34.000 100

.137.000 34.000 39.000 38.000 36.000 39.000 36.000 35.000 39.000 36.000 Transform: Untransformed 1-Tailed Isotonic

'Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Mean N-Mean D-Control

32.000.,'

1.0000 32.000 29.000 35.000 6.074 10 34.133 1.0000 11.3 32600,.

1.0188 32.600 30.000 35.000 5.254 10

-0.806 2.287 1.703

'34133 1.0000 226

`:33.500:6' 1.0469 33.500 31.000 35.000 5.122 10

-2.014 2.287

'1.703 34.133 1.0000

'45.2

.34.200 1.0688 34.200 32.000 36.000 3.850 10

-2.954 2.287 1.703 34.133 1.0000 72.6' 35.600.

1.1125 35.600 34.000 38.000 4.016 10

-4.833 2.287 1.703, 34.133 1.0000 100 36.900 1.1531 36.900 34.000 39.000 4.856 10

-6.578 2.287 1.703 34.133 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.864555061 1.035

-0.055127545

-t:11981957 Bartlett's Test indicates equal variances (p = 0.88) 1.7678653 15.08627224 Hypothesis Test (I-tail, 0.05)

NOEC L[EC ChV TU MSDu MSDp MSB MSE F-Prob df Durinett's Test 100

>100 1

1.703244044 0.053226376 34.226666671 2.774074074 5.4E-08 5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point-SD 95% CL Skew I

'dlo IC15' IC20t IC25 IC40 IC50

>10v0

>100

".>100

>100

>100 k

sqnOl_O02-10-O9data

Environme'2n.Lu Test &

liIo.nc.

TVA / Sequoyah Nuclear Plant, Intake - Non-treated February 10-16, 2009 Statistical Analyses V:

Ceriodaphnia Survival and Reproduction Tesi-Reproduction Start:Date:.

2/10/2009 Test ID:

CdFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Intake End Date:

"2117/2009-":

Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoriing Report.

Samole Date:.*

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubi Comments:

Conc-%

'1, 2

3 4

5 6

7 8

9.

10 D-Control'

, 32.000 o

31.000 31.000 33.000 33.000 31.000 33.000 32.000 31.000

30.000 100 34.000 37.000 35.000 33.000 37.000 40.000 35.000 34.000 37.000 35.000 Transform: Untransformed 1-Tailed Isotonic

-Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD" Mean.

N-Mean D-Control 31.700 1.0000 31.700 30.000 33.000

- 3.342 t0 33.700 1.0000 100 35.700.

1.1262 35.700 33.000 40.000 5.763 10

-5.466 1.734 1.269 33.700 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Slhairo-Willgg Test indicates normal distribution (p > 0.01) 0.901544869 0.868 0.809339798 1.387605747 F-Test indicates equalvariances (p = 0.06) 3.772277117 6.541089535 Hypothesis Test (1-tail, 0.05)

MSDu MSDp MSB

-MSE F-Prob.

df Homoscedastic t Test indicates no significant differences 1.26901657 0.040032068 80 2.677777778 3.4E-05 1, 18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point SD 95%CL Skew IC05

>100:

Id15 IC20 IC25 IC40 IC50

>100

>1060

.}>.too

>100

. >I00

'S -~.

7,4:

.;;(

sqnlOJ_02-10-O9data

Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated February 10-16, 2009 Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Daily Chemical Analyses S IProjject number:.

5246 Reviewed by::U+__-----

I.

Y

-~

Concentration IParameter Day 0 Initial I Final Day I I

Day 2 I

Final I Initial I Final 4~

pH (SU) 7.891 7.701 7.781 DO (m /IL)

Control Conductivitv (umhos/cm) 4-Alkalinity (mn.L CaCO3) t 7.71 324 63 90 24.7 Da'3 In ia nta "1- ---- ----

Hardness (mg/L CaCO3)

Temnerature ('Ci 24.71 24.6 24.51 25.01 24.7 pH(SU)

.7.88j7.79 7801 78f 7-861

.75J 7.81 7.85__

7.86 7761 DO (mg/L) 7.8 1

7.61 7.7 791 771 7.7 7.6 7.9 11.3%

Conductivity (puhos/cm) 302k 312 305 300 304

. Temperature(AC) 247124.61 24.724 24812481 24.7 247 250 24

.9

[ni Ig1ll 7 89 7.81 7.871

7.86 7.891 7.74 7.811 7.85 22.6%

DO (mgfL) 1 7.8 7.61 7.7I ~

7.91.U 7.1 ni 9

Codutiviy (imoscm 290 w

300 w

23 29 Temnerature t*'C 24.7 24.8 24.71 24.8 24.81 24.8 24.71 24.9 H (SU) 7.89 7.85 7.89 7.87 7.90 7.75 7.81 7.86 7.88 7.888 7.

7.DO (mWL) 7.9 76 7.7 7.8 8.0 7.7 7.9 7.6 7.8 8.5 7

45.2%

Conductivity (unhos/cm) 264 0

26 268 262

...... Temperature (?C) 24.7 247 24.7 24.7 24.8 24.8 24.7 24.7 25.0 24.8 25 11 (SU) 7.92 7

5 7.92 7.87

.. 79 775 7.81 7.7.

.9.

0

.788 DO ('aWL) 8.10.

7.8 8.01

7.

8.0 7.9.81 8.0 8.57

.7.

7,*

7.75 7.5

-78 72.6%

O Conductivit " (mhos/cm) 2 2

-.233 249 4 _4.7

2.

24 2

.8

b.

100%

Alkalinity (mg/fCaCO3) 74 Hardness (mgfL CaCOt

)

86 Total Residual Chlorine (n,/L)

<0.10

_______Temperature

('C) 24.71 24.7 24.81 24.9 24.91 24.7 24.7 24.6 25.11 24.91 24.92 PH (SU) 7.941 7.901 7.951 7.9 794 7.76 7.85 7.891 7.901 7.82 7.87 7.1 DO ('A) 8.21 7.6 7.7 7.8

.8.21 7.6 8.0 76 7.9 8.1 8.5 7

Conductivity hunhos/cm) 206 100% Intake : Alkalinity (inii/ CaCO 3) 74 Hardness (mg/L CaCO 3) 92 Total ResidualChlorine (mg/L)

<0.10

<0.10 Temperature'C).

24.7 24.6 2.6 24.8 24.7 24.7 24.7 24.9 24.9 24.9 24.8 25

,-i.

~

w:

I:

a L,

I Page 6of 7 Species: Ceriodaphhiadubia

',Date

01.

10 0' Client: Sequoyah Nclea'r Plant Non-treated-1

~

A-Lt" Day V

Analyst I Concentration, r

"77 r

CONTROL PH KS.U.)

DO (mg/L)

Conductivity (jimhos/cm)

Alkalinity (mgz CaCO,/L)

Hardness (mg CaCO1/L)

Temperature ('CQ I.

7~

7' PH (S.U.)

ii Thi-q I7.~o I

11.3%

DO (mp/L)

Conductivity 52 (jimhos/cm)

I vAb 60 TA.-

Temperature (°C) 335 1A.~~ L'.k 9* -.

pH (S.U.)

f'~(

I 7-~~

2216%

DO (ma/L)

Conductivity (iimhos/m rn

______ I 370 Temperature (C) pH (S.U.)

SI

~

i47-j A

j~

-~I 1

45.2%

DO (m/L)

Conductivity (pmhos/c )

c~§I~5 2-Q 9,-)

=

2.10 II

.-~

I Temperature (TC)

I I

4-I

[I I

II pH (S.U.)

~I*1 47-~

I'c2 q IF

-I

1-72.6%

DO (mpgL)

Conductivity (iimhos/cm) 12-Temperature (oC)

I DH (SU.U

§~~I II-i~-~

iqq

-4, 1 3

1 I

I.*..*,-"

I

/

I 19 M4 100%

DO (mgfL) 8A II Conductivity (jimhos/cm)

Alkalinity (mg CaC0 3IL)

Hardness (mg CaCloiL) 6m/L TR chlorine (mg/L) 4 <0. 11

-'4 2.9 1 Temperature (°C) 21J.7 I U U

Ii ~4o6'

~

pH(S.U.).

100% Intake DO (mg/L'"

LO 3.

tonduciivity A

(jimh os/cm)

O:(~

Alkalinity (mg CaCOj/Ly -

'Hardnes*s (Mg CaCohl3

)

___0,__

TR chlori'ne.(mgfL-)

.p 0

?q.1 Temperature ("C)

I..

age 47 of 98 I nitial I

Final I

Initial II Final I Initial

- jt4C.Pu #.._4At~b 14 OEA A".W31 IMF

)

APage 7 of 7

.*',.*.....,*,*s.,o.**

...........,' ': :.**s*:*:;

,.i*

~~3 V

Species: Ceriodaphnia dubia Client: Seqiudýah-,NuclearPlaiin't-Non-treated D"te" 2 - I,(jL--P.

I Day I

Concen-n tration Parameter CONTROL DH (S.U.)

II DO (mi/L) 4 -C----

Conductivity (jgmhos/cm)

Alkalinity (mg CaCO3 /L)

Hardness (m2 CaCO,/L)

Temperature (TC)

PH.

-t.1-m7 i

11.3%

DO(mgL) 1.1

-1.9

-Z

.4+

7O Conductivity 1C-(_nmhos/cm)

Temperature (°C) zq.

IS 1 0 LA

. f, "

PH (S.U.)

Sl 8

7B 4

.q-7.99 22.6%

DO (mg[L)

Conductivity 7

(prmhos/cm)

Temperature(°C)

( T) 1

".o 5./, PH (S.U.)

/ q t

",8o aB

,q 1

45.2%

DO (mg/L)

LPs Conductivity (pimhos/crn)

Temperature( (C)

._0 IS pH (S.U.)

7 1 (

72.6%

DO (mg/L)

Conductivity (jimhos/cm) 1 Temperature (C) 14 1

CA 15 II I

-N -?II

.1 I

I I

I Ii pH (S.U.)

I I.Ptq I

IAD1 B'1.001I 1.N I -

1C I

II I I

4

~I.

100%

DO (mg*L) 2.0 0

Conductivity (pmhos/cm)

Alkalinity Hardness (rag CaCO3/L)

TR Chlorine (mg/L)

Temperature (0CY-.`: I

-.6 T Ls. t I.

,A,-"l I-- -,11 --

-4,%.-.

I 100%';

intake pH (S.U:),- -

1 1 II5

ý 11 -

1r.

~~1I*~?~.1 r~j~4 -B 1.8Q I

"~II

~i I

-DO (mKf)

Conductivity (pmhos/cm)

Alkalinity (mg CaCO3/L)...

Hardness-..

(mg CaCOJ/L)

TR chlorine (mg/L)

Temperature (°C)

<0. I z

lq,'i 241.

91 2

I I'age 48 of 98 al I

Initial 1

14-4. I 1

FSi-.

I nt a al I

Initial 11 Final I

Initial*..

Fina'l I

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species Pimephales promeask Client: TVA C.,.ounty: Hamilton, Facility: Setluovah Nuclear Plant Treatment: UV.treated NP D ES #: T N 6002 6450V 101C

~'

Pr~oject#:

S~

Page 1 of 6 Dilution preparation information:

Comments:

Dilution prep (%)

11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 565 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 22 t7.5 1935 1370 685 0

to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences.

Test organism information:

Test information:

Organism age:

Lot RoU4 Ot-Randomizing template:

6 L* e Date and times organisms a-oL-Oc%-0 ci o Incubator number:

5 were born between:

Organism source:

AmRO( I,'rC.A 0e

-L(A.-cr-4 Artemia lot number:

ea O~qU Transfer bowl information:

pH Temperature.=

'C Total drying time:

__4-_t___

"1,% 7V

".q.

Date /Time in: c','-dL.

1111i Average transfer volume:

Date /Time out: O.-U -00I Itio Oven temperature:

aof" L Daily feeding and renewal information:

Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time I

02-It-o

&S

%S t55 0t-ob-oi o6 o

)

.. O9

, 0 1407-2 Oti-I-c A 015 io 10-A I 0'-LO%1-O4 9

0~

7- % ki4 4-S d

32 O.

C 0400

%%Ab I\\_\\

a

-Ca-o* oý cr-i. 'A -

2-ig-P09 OAOO o

1000 01,10-01 (A

4L%51.

1 f

3___

0,.s o-di 0p

,oD

\\

cr'l-i-o9dP a

o S-t-OaS 6

G2Z-b*,b

,40Q0

/000 O-z-IO-_O%

I

~

" o(Im

'sk0

=

0Icn1-09 EO 6

2L

'6 i

nro.inormr*, on.__

Acceptance criteria,.

nSummay pf testend oints:7'

% Mortalit..:

,-*20%

7-da

67.

7-d7,

._Average w'eight per-initial larvae q,',o.

x"Zo7

-NoEC;$).:,

Average.weight....s...........va62. CI.9 :/

-0 aia:,, ILOEC."___"___.....

'Average weight p~er survving,,:

a!' --

a:*

Qz

.. 2

'2 m

e**lu"*'i i

' ':'Z*.

'* i/!.*.5 71%

'ChV.

'IC;s 7a v, I -

49 of 98

.I ETS Page 2 of 6 I,

Species: Pimephales prom elas Client:. TVA ISequoyah Nuclear Plant - UV-tre~ited.

-27. Date:,-t-6Z o.O 3/4~

....Survival andGrowth DataW.">'

"Day

' CONTROL.

11.3%

22.6%

A

' -B C-.

D E

F G

H I

J K

L 0

10 10 10

/D

/0 10

/0 1Q I0 D0 /0

/Q 1

1010(0 0(000t o/0/

0(

O 1 0102)10 2

1t1 lo lo to o

to 0 o I0 to, to

/o 3

115 (0

(0'

-I 10 tO 1CI

/0

/0 I C(

,10 ID 10 I0- 1t to I

Io

/05

/O /0 6

J

1)

/0 /0

/0

/0 (0

/Q

/0 o

/0 10 to

/0 to 0

0 10

/0 1

C0 10 A = Pan weight (mg)

Tray color code:: OVO.._.

Analyst:

1 ".3 5

lq.

A4 lq.11

[-(09 1q

.9 13 Z I

.3q 1q.07 15,7.t Date:

02-ip-Ogq B = Pan + Larvae weight Analyst:

PA Lr l.I.o 1,6 o 4 Al I -qý4 Date:

011-l C = Larvae weight (mg)

=A-B

(.%X IDAPkS t..,42. 1.2.0 1.a 10k 1

.4 J n.'-o

,t.b (0,

Weight per initial number of larvae (mg)

= C / Initial number of larvae N'

%9 0'

0

'a t7 x*2

'0 0"

O" 0"

t)"

%9

.e 50 of 9*

i:I r

ýverage Percent veight per reduction nitial from control

0.

2 l number of

(%)

ar'vae (ra g) *..,.

~~~~~

i

-6 SComment codes:c= clear;d -dead,fg fuiagus, k killedn =mmissing, sk -s icksm unusually small,

= unusually large.d&r. decanted andreturned, w' wounded:"

data cul s

..,.. :".'d....

-review'"ed:

8 I

Comm~entsh.'.~j'f'

)

iF" 9

EflVPOPUVflIQM Tntr.9 SOlutfant.

nc.

Page 3 of 6 5

5 Species.: Pimeph ales promelas Date:

Client: TVA / Sequoyah Nuclear Plant - UV-treeated, Survival and Growth.Data

~Day 45.'2%'

72.6%.--

10%

1

________M

-N 0

P Q

R S'T U

V-x x~

10:"'10 o"'/

"0

)

/0

'O 1

1'0

/0

/0 16 1/0 0

ID 10

)0 I0 1/0 10 10

/01 10 2

1-)

10 (D 10

/0 10 I0 1

I t0 1((

IC0 10 3

3t

/0 iL)

/0) t(Q

/t L0 t0 I/0

/O 10 4

/0

/

o

/

t C0 10

/6 i0

/0 o O

/0 I C0'0 I0 1I 0C3 1(

to 0

t 0to to 6

10 (0

t It C) to C

b i 0

'0 (0

/0 I0 (0

I0 1 C)

A = Pan weight (mg)

Tray color code:: O*aw're 5.1

. t I3.t8 13.01 Nqe13 tq.1'4Act.l t3.1 'M'41 t'.*,A A nalyst: U

.I Date: O2-itc-a',

I II B = Pan + Larvae weight Analyst:

Date:

O'j IS-OA C = Larvae weight (mg)

=A-B S 1.

S 1

2 S

1 01 Weight per initial number "N

of larvae (mg)

\\0.

8 C

4' O

I" nm" of la rov Average Percent weight per reduction initial from control o.sZ 0.*q a A7.

number of

(%)

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, lg,7,unusuallylarge, d&r decanted andreturned, w 7wounded.

Calculations'aftd daiýaie

-;,C.*.,:

4' )

View 51 of 98 Comments-:"

4 1L',C

.;(,*f-'*':'

i 6d: A 77 1-C

C

-T.

Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - uV-treated Date:

-L ti-I -61 a

'Surviva[at ay-.i00/.

lintake Y

Z

.AA

-BB 0

_/0

/0

.t0 1

I)

/3

/0

/0 2

(C 10 10 ID 3

to tI

)0 /0 14 to t 1 /

1) 6 IQ 10 1

7 tsm isl A

Pan weight (m) t Tray color code:: W-S Analyst: Lk:-.

Date:

.O2 Opl B = Pan + Larvae weight (rag) q5 Analyst:

1 Ja 3 o Date:

o*-'W Q'O C = Larvae weight (mg)

=A-B W eight per initial number A

A, of larvae (mg)

= C / Initial number of larvae Average Percent weight per reduction initial from control 0.1.

number of

(%)

larvae (mg)

Comment codes: c,= clear, d = dead, fg.= fungus, k,= killed, m missing, sk -sick, sm-unusually small, <'.

... = unusually large, d&r = decanted anfd returned, _w>= wounded.

Calculation and dat.

.revie S-.

r*..

-4.

e 52 of 98 Comments:'

TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated February 10-17, 2009 Environmental Testing Solutions, Inc.

Pimephalespromelas. Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Proeit numbei; 5246 Revelwed b Not for Compliane Asse..ment.

temal Laboratory QC A

Coattraiona(%..

Repiictm Iitalnumber of ial antumber of A =Pan weight BF=.a +trv ae w

areaiht(1ga Wdghl/Surviviag Mean reighi Cafficieatufvariation Weigbt/ Inital number Ma survial

. Mean wigpt/

coafnt of Pearcnt reduction from larvae larvae (ag) weightt(rag)

-A-B anbedlardat wag) Survivinignumherof (am v"",r*.

of larvae (n)g)

Iati.al anabe'o veariatino (-

-0a.

coatre (%1 iaaval (mg) iarvac nwJ

-A 0

10 14.76 2087 6.11 0.611 0.611 B '

0 10 14 3 20.64 6 30.

30 0 6 9410.630 o o Control 1434 4

6.30 0.630 0.629 4.1 100.0

'6.629 4.1 Not appllcable

_otr_.C_

".10 10 13.58 19.68 6.10 0.610 0.610 No p

". D 10 10 14.14 20.79 6.65 0.665 0.665

_ _E "10

'l 10 14.11 20.53 6.42 0.642 0.642 11.3%

_F 10 10 11.69 18.89 7.20 0.720 0710 6.5 0.720 100.0 "

0.710 6.5 12.8

' G___

10 10 14.43 21.72 7.29 0.729 0.729 6

H 10 10 13.89 21.36 7.47 0.747 0.747 1

.10 10 15.61 22.25 6.64 0.664 0.664 226%

J 10 10 14+34 21.74 7.40 0.740 0.676 6.6 0.740 100.0 0.676 6.6

-7.4 K

10 10 14.07 20.43 6.36 0636 0.636

_L 10 10 13.22 19.85 6.63 0.663 0.663 M

10 10 14.67 21.83 7.16 0.716 0.716 4502%

N to 10 15.85 22.07 6.22 0.622 0.608 13.9 0.622 100.0 0.60i

13.

i 3.4 O

10 10 15.32 20.48 5.16 0.516 0.516 P

.10 10 14.45 20.22 5.77 0.577 0.577 Q

10 10 13.68 20.10 6.42 0.642 0.642 72.6%

R 10 10 13.01 18.72 5.71 0.571 0632 7.0 0+571 100.0 3.62

7.

0 0:5 S

1o 10 14.93 21.32 6.39 0639 0.639 T

10 10 14.71 21.47 6.76 0.676 0.676 U

to 10 14&64 20.90 6.26 0626 0.626

]

1V 10 10 13.95 20+49 6.54 0.654 0.654 100.0

.00%

W 10 10 14.41 20.88 6.47 0.647 0.647 0

4.3 X

10 10 14.26 21.14 6.88 0.688 0688 Y a 10 10 14.62 20.29 5.67 0.567 0.567 100%Z 10 10

.12.97 18.98 6.01 0.601 0.618 10.9 0601 100.0

_,:0.618 10.9 1.8 AA 10 10 13.67 19.53 5.86 0.586 0586 Ba

10 10 13.43 0607.17 0.717 0.717 000011101:

Donnell'& MSD v, loe:

PMSD:.

MSD =

PMSD 0.0838 13.3 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake; Dunnett's MSD vidue::'- a PMSD:

Lower PMSD bound determined by USEPA (10th percentile) 12%.

Upper PMSD bound determinedby USEPA (90th percentile) 30%.

- 5.

- I

_1

'd' UsFPAý 2001 b)ý Lower wd upper PMSD bounds were detertnined from the loth and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaborta06 Variability Stu y ýOSEOA, 200l.;

USEPA. 2001a, 2001b. Final Report: Interlaboralory Variability Study of EPA Short-term Chronic and Acute Whole Effluent toxicity Test Methods, Volumes I and 2-Appendix. EPA-821 -B-01-004 and EPA-821-B-01 -1 005. US Environmental Protection Agency, Cincinnati. OR

TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated En~vironmi-ntal Testing Sotlutions, Inc.

February 10-17, 2009 Statistical Analyses 1 -

1.Larval Fish Growth and Survival Test-7 Day Growth Start Date:

.2/1012009.--+

Test ID:

PpFRCR Sample ID:

TVA / Sequoyah Nuclear Plant, Outfall 101 t.'End Date:

2/17/2009.7,:

Lab I1):

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring' Report L

+Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

Conc-%"n-A1" 2

3 4

D-Control 0.61,10 0.6300 0.6100 0.6650 S

11 0.6420 0.7200 0.7290 0.7470 22.6~.@

0.6640 0.7400 0.6360 0.6630

'45'2 0.7160 0.6220 0.5160 0.5770 72.6 0.6420 0.5710 0.6390 0.6760 100 0.6260 0.6540 0.6470 0.6880 Transform: Untransformed 1-Tailed Isotonic Conc-%

.Mean N-Mean Mean Mi Max CV%

N t-Stat Critical MSD Mean N-Mean

'D-Control:.

0.6290 1.0000 0.6290 0.6100 0.6650 4.086 4

0.6714 1.00001 11.3 0.7095 1.1280 0.7095 0.6420 0.7470 6.537 4

-2.315 2.410 0.0838 0.6714 1.0000 22.6 0.6758 1.0743 0.6758 0.6360 0.7400 6.623 4

-1.345 2.410 0.08381 0.6714 1.0000 4r5.2 0.6078 0.9662 0.6078 0.5160 0.7160 13.860 4

0.611 2.410 0.0838: ',

0.6312 0.9401 72.6 0.6320 1.0048 0.6320 0.5710 0.6760 6.961 4

-0.086 2.410 0.0838 "0.6312 0.9401' 100 0.6538 1.0393 0.6538 0.6260 0.6880 3.938 4

-0.712 2.410 0.0838:

0.6312 0.9401 Auxiliary Tests:

Statistic Critical Skew Kurt Shapiro-Wiltks Test indicates normal distribution (p > 0.01) 0.98361433 0.884 0.19270246 0.87105609 Bartlett's Test indicates equal variances (p = 0.37) 5.42555952 15.0862722 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob

- df, Dunnett's Test.

100

>100 1

0.08379661 0.13322196 0.00540594 0.002417958 0.09521794 5, 18 Treatments vs D-Control Linear Interpolation (200 Resainples)

Point SD 95% CL(Exp)

Skew IC05 41.450 160O 2ICIS C20 IC25 JC410 IC50ý

>100

>100 sqn101_02-10-09data-uv

T 0

TVA / Sequoyah Nuclear Plant, Intake - tUV-treated February 10-17, 2009 Statistical Analyses

.En wvironmlenltal esting SOIUtIonS, Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date:

2/10/2009".

Test ID):

PpFRCR Sample ID:

TVA/ Sequoyah Nuclear Plant, Intake End Date:.

2/17/20090 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

DMR-Discharge Monitoring Report Saminple Date:'

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:'

, Cone-% ýt

1.

2 3

4 D-Control 0.61:10 0.6300 0.6100 0.6650 100 0.5670 0.6010 0.5860 0.7170 Transform: Untransformed I-Tailed Isotonic Conc-%

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD.

'Mean N-Mean D-Control 0.6290 1.0000 0.6290 0.6100 0.6650 4.086 4

,l 0.6290 1.0000 i00 0.6178 0.9821 0.6178 0.5670 0.7170 10.945 4

0.311 1.943 0.0703 0.6178 0.9821 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.85388428 0.749

-1.514357215 2.37378198 F-Test indicates equal variances (p =.0. 15) 6.91965199 47.46722794 Hypothesis Test (I -tail; 0.05)

MSDu MSDp MSB MSE F-Prob

2.

df Hombscedasticet Test indicates no significant differences 0.07027929 0.11173178 0.000253125 0.002616125 0.766281605

.1,6, Treatments vs lD-Control,.

Linear Interpolation (200 Resamples)

Point SD 95% CL(Exp)

Skew tC05

'dIo IC15 IC201 IC25 IC40 IC50

>100

7-

1 -sqnlO,O]02-IO-O9data-uv,

Environmental Testing Solutions, inc.

TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated February 10-17, 2009 Pimephalespromelas Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Daily Chemical Analyses Project number:

5246 Reviewed by:

Concentration Parameter Da 0 Da 1 D

2 Day 3 Da Day 5 Da 6 Initial Final I nitial Initial FInitial I

Final initial InitialF.

Final Initial Final pH (SU) 7.871 7.701 7.80 7.6 7.74 7.2 7.801 7.721 7.87 7.7 7.911 7-73 7.82 7.40 DO (m.L 7.81 7.81 7.8 76 8.0 747.61 7.4f 7.8 778.51 7.41 7.71 6.7 Conductivity" (mhos/cm) 309 3

310-31139 303 Control Alkalinity (mg/L CaCO3) 62 63 61 Hardness (mg/L CaCO3 )

90 94 90 Temperature (°C) 24.7 24.6 24.6 24.5 24.7 24.6 24.7 24.6 24.8 24.6 24.8 24.6 24.8024.6 PH (SU) 7.83 7.68 7.85 7.04 7:15 7.61 7.82 7.76 7.89 7.52 7.91 7.74 7.85 7.42 Do (7.g/L) 7,7 7.5 7.8 7.6 8.0 7.3 7.6 7.4 7.8 7.5 8.5 7.5 77 6.6 11.3%

C onductivity (pim hos/cm )

2982 310 304 308 30 1 296 313 T em perature (°C )

24.7 24.4 24.7 24.3 24.8 24.6 24.7 24.6 24.8 24:3 24.9 24.4 24.8 24.6 PH (SU) 7.82 7.71 7.86 7.04 7.15 7.65 7.83 7.72 7.90 7.59 7.91 7.75 7.85 7.42 22.6%

DO (m.g.)

7.8 7.5 7.9 7.5 8.0 7.2 7.6 7.4 7.8 8.5 7.5 7.8 65 Conductivity (mus/cm) 287 2993 294 288 287 303 T em perature (TC) 24.7 24.4 24.7 24.2 24.8 24.5 24.7 24.6 24ý9 24.3 24.9 24.7 25.0 24.3 pH (SU) 7.82 7.74 7-88 7.03 7.15 7.70 7.83 7.74 7.90 7

7.91 7.87 DO (mg/L) 7.8 7.5 7.9 78.0 7.7 74 7

8 85 7.7.96.5 452 7.5 7.3 74 78 7.85.

6.579 Conductivity ([,mhos/cm) 263 276 271 268 266 279 Tem perature (T )

24.8 24.2 24.7 242 24.8 24.51 24.7 24.4 24.9 24.5 24.9 24.71 25.0 245 pH (SU) 7.85 7.751 7.89 7.02 7.15 7.71 7.821 7.78 7.91 7.64 7.92 7.74 7.881 7.43 DO(ng/L) 8.0 7.51 7.9 7.5 8.0 7.3 7.7 7.5 8.0 7.7 8.5 7.4 7.9 6.4 72.6/,

Conductivity (imhos/cm) 235 245 239 23 239 234 I245" T em perature (°C )

24.8 24.2 1 24.7 24.3 24.8 24.7 24.7 24.3 24.9

-24.4 24.9 24.5 25.01 24.4 pH (SU) 7.90 7.771 7.92 7.02 7.14 7.74 7.80 7.76 7.931 7.61 7.92 7.77 7.88 7.43 DO (mg[L) 8.01 7.51 7.91 7.6 8.01 7.3.

7.8 7.5 7.91 7.71 8.41 75 7.7 6.4.

1 Conductivity (pmbos/cm 210 218 212 2

08c208 2m Alkalinity (mgfL CaCO3) 71 72

_______Temperature

('C)

.24.81 24.41 24.7 24.2 24.91 24.61

.24.81 24.51 24.91 24.41 24.91 24.5 25.1 24.4 PH (SU) 7.89 7.771 7.89 6.99 7.14 7.83 7.68 7.75 7.92 7.55 7.87 7.78 7.87 7.41 DO OnigJL) 8.41 7.41 7.8 7.4 7.91 7.31

. 7.81 7.91 7.41 8.71 7.41 7.81 6.41 Conductivity (pinhos/cm) 208 21 2082829(9 1

10 0 % I n ta k e A lk a lin ity ( nm/L C a C O 3 )

74 7 2 Tem perature(C) 24.7 24.2 24.7 24.6 24.9 24.6 24.8 24.4 25.0 24.7 25.0i 24.4 24.9 24.4

Species: "Pimepi Client: TVA/ S Daily Chemistry:

h alespromela

ýequoyah N dclear Plant-: UV-treated Page 5 of 6 Date:

".d 16.- o Day 2

o 1týc.

I t ALt\\

1 I.

onenraio Pal st arameter Concentration Parameter.

.CONTROL DH (S.U.)

0,;,

I

"/

2bo.:

1

,0&b I..-.TtL - II L tz.....a...._

DO (mpL)

Conductivity (jimhos/cm)

Alkalinity (m CaCO3/L)

Hardness (mg CaCO3iL) r4 fl-)

3319 16 L b (9b qA A

rTemverature (MC)

I a DH (S.U.)

-I Oq

-4 )S" I -

k 11.3%

DO (mra/L)

Conductivity I

'U%%

3_.

3 Temperature (°C)

"--I "t It I

42

.11.

4.

a DH (S.U.)

1, 2-1 7,"4I 1

'~

T IV -4,04L I

Ti i T 106~S 1

T 22.6%

DO (mgfL)

Conductivity (J.Lmhsc m) 2 1' k -,

"t~q~

1,*.q

-q,1 "tqL

~

q IQtq Temperature ("Q L

I.

pH (S.U.)

Z_ 10-LA-14 Lq.1 TA I 4,

-1 ct o 3 45.2%

DO (mg/L)

ConductivityI

-zq,5

'4 II0-.

t* ?,

-t.

I z~

Temperature (°M h

!H (S.U.)

ýý 7-

-S

-4,

7, 14 7-X 72.6%

DO (mP/b)

Conductivity asoq1 L4.2..

93q 2

t 4

Temperature (°C) k p)H (S.U.)

100%

NmotA eATeD DO (m'g/L)

Conductivity (4mhos/cmn)

l Alkalinity

(-1g CaC 3 )1\\

Hardness (mg CaCO3IL) 8__

TR chlorine (mg/L)

< 0.1.0 6.o 2VC

_6._0

, "z~q.2U "q

7-A.

zq II TemneratUre (°C).

_II _______- _

,,' ~

~

em e at r

_ ")

II niH (S.U."

O,- t II =

I :.g'q II qc, ~1.L

-1 1 100% fntake

-w*,jer eb -- 1o pH- (S.U...1

.)"

I a

'r DO (muJll)'

,4,

II Conductivity (limhos/cm)

__._____)_'

AlkalinitY. '"

(mg CaCOWL)

Hardness (mg CaCO3/L)

TR chlorine (mg/b)

< <0.10 k4 nal Initial Final 1A_.t,7

'k*;,,

,t...

Temperature (°C)

I

4,Lt 11 I

m Initial Fnl Initial I

Ir

,,*.

e*

57 of 98 57.of 98 re'h.,

r ¢,U6oN C.v,

0 Page 6 of6 9T S

."~-

,. nmtj.. ~ gSo,.~Ios.~4n~,

Species: Pimephales-:promelas Client: TVA / SequoYah Nuclear Plant -UV-treated Date:

O?..-l rd-cA I

IlDa-

,1, Dav-II.o,,,

I

.A ctAra" 11 :Y4ýcý 1'.

Conceit-,

Parameter tratio

~0 CONTROL oH (S.U.)

-1*

1

.Z.C)I- ' 7i

-z7`1 I

II I

DO (mg/L)

.Conductivity

.(iimos/cm)

Alkalinity I

(mg CaCO3/L)

Hardness I

(mg CaC0 3/L) 1-6 -- 1 1 7.8

~iA10

,A.L.

,tl.2 I 1-J-17' I

313 Temperature (°CQ II '71A.

I II I-LA."

I pH (S.U.)

k..

1.1 r

1-

-2

II IS PIS 11.3%

DO (mg.L)

-1 Conductivity 3

(Amhos/cm)

_ Temperature ( °C)

L'.

"L4..

7 U 91..

A.

O

\\

p H (S.U.)

7.S9

-1.

-1 2 1 Z S I q " 1

.1l R S 22.6o DO(mg/L)

.1.1.8 1.<

_79 Conductivity tO3 T e m p e r a t u r e (

°C

)

(.Q

. I

' 7 -Q A n 1 6.

0.

PH-(S.U )

-1 09-

-_81 45.2%

DO (mgfL)

Conductivity

-LP (rthos/cm)

- -T em perature ( Q 1 8.1 7...

'ICA 1

0 PH (S.U.) 2..

10 ci1 L L.

72.6%

DO (mg/L)S0S; Conductivity 21-6

.c (4rnhos/cm) 4 Temperature ('Q 1.'-I1 1A.

~

2

-NL' v2.jI U.4. D

.0.

1H (S.U.)

-I-go I.-I

.C13 q'q z

-i-BG ý 1'4.qý 100%

DO (mg/L)

Conductivity

([umhos/cm)

Alkalinity (nig CaCO3/L)

Hardness (Mg CaCO3/L)

TR Chlorine (rng/L)

,Jemperati ret(OC..).

tLO 10.l F;' L'ýVd 6

pH (S.U.)

.I 1-.6j?

1 -ig 1, T 9z 100%

II"}intake

,DO (mg/L) -.

Conductivity' Alkalinity*,

(mg CaCO3/L)' -:..

Hardness TR chlorine (m gL)

Temperature C(C) i~0.10 I

a II I

I II tge 58 of 98 I

Initial II Final I

Initial II

V Environmental Testing Solutions, Inc.

Analyst F -*y.-.

Date :analyzed Page Page I of J Total ReSidual Chlorine-(Orion Elecfiode Method, Orion 97-70)

SMatrii-x:' Water, RL = 0. 10 mg/L,

Meier: Accumet Model AR25 pI/Jon Meter 4... 4.,-;:*.,

Aid ereagent: I __ _

1_

]

)'O* *-*

n..-

'+

Acid reagent;[ J(\\

3*b Calibration:

'~

I IM.S-S S71 11 1, 70'ir

/7 I.Om g/L I Reference standard number I ra--

i-'

-I Note: For samples with a residual chlorine of > 1.0 mg/L. the calibration range must be adjusted to bracket the chlorine levels of the Samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number.

(rag/L)

(mg/L)

(acceptable range = 90 to 110%)

o~..50 o ' rq l~f S19 Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD = ((S - D) /((S+D)/21) x 100 number t

C.

(mg/L)

(acceptable range 10%)

Duplicate D

O.Oo08p Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number

-"(mg/L)

Blank (should be = <0.10mg/L) 7777

_Z 0,___-_

Vo--t0 luO6o.

),

0i"1t0.O.

(AM.p ftorik 4,

cl4I) swt~ p44 4 4 C

01 ux=0______6~

&e.

2 ~

M.

oto' 4 6

(1& &J4J~ 16"~~~W

~

0,~b)

<4*. 4..

I..

4

.4,4..

..~

4 4

A 4.

f Note: All'samples were analyzed in excess of EPA'-redommen-nded holding time (,15-miinutes) uriless otherwise-noted ",

4 L..boratory control standard:

444. ',

44 \\.,p,.4 4.4,4

?4C.. `;.

Reference standard,,+'..,

Truevalue (TV).

Measured value,(MV)

-RS

- Mv)/ TV x100 :..,

1 number 4 UP 70

.(mg/L) 2

,44-']04.:.4

,(

blerange.90t00.)

'0.50

~

T 5'3 -67 age 59 of 98 Reviewed by I -X AI Date reviewed I d7-[lo-Oj

0 Page __

Page I of Errvtroemnrn tal Testing Solutions, Inc.-

Total Residual Chlorine.

(OrionElectrode Method, Orion 97-70)

?

Matrix: Water, RL= 0.10g/L

.10.

Meter: Accumet Model AR25 pH/Ion Meter Analyst' [:-:

Iodide reagent Dateanalyzed 02/14.9y i

Acid reagent:

Calibration.

I tAss. S1 I ol Olm-7 7

Too 1.O7m I

I Reference standard number i

4rA S

ý-441 1;

ý 63;4 ý A I

I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:.

Reference standard True value (TV)

Measured value (MV)

% RS = MV /TV x 100 number (mg/L)

(mg/L)

(acceptable range = 90 to 110%)

0.50

.16q Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD= ((S - D) /[(S+D)/2]) x 100 number 14n Cotftl

(=/L)

(acceptable range =:k 10%)

Duplicate D 4,(o lqq i

Sample measurements:

.Sample Sample ID Sample characteristics Residual chlorine number mg/L)

Blank (should be= <0.10 mg/L)

'DZlr/L O * *J"

--,hc.

a Lo o

"o o

IoJ.

J oc)L.zq Mozl:.o_

OOP ts_

6,0 q))

Note: 'All samples were -analyzed in exce~f,`f EPA rieconunrended, holding timiel (1 minutes) unless-cotherwisenoted.

S4:;.

`7 L'aboratory. c~ntnl.stdndardJ;:QL A /-~Th.4 Reference standaid

.`

Truealue(

Ii.e'asired~value(V RS MV!T if (1112()

(cceiptab exange..,9040rll I

ii

~0.50 6

~

))4

.I I age 60 of 98 Reviewed by Date reviewed 07 jJ

$~EnI vlranmentoi Tesding SolutIons. Inc.

Page 70 Page __

of 2.

Total Residual Chlorine (Orion Electrode"Method, Orion 97-70)

Matrix: Water, RL= 0. 10 mg/L Meter: Accumet Model AR25 pH/Ion *Meter Iodide reagent]

7.,

Acid reagent:

Date analyzed L-

_ -)

lO Calibration:

0.10 m g/L 1

1.00 m gfL Reference standard number

/

ss 1

Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number (mg/IL)

(mg/L)

(acceptable range = 90 to 110%)

N) 0.50 0.55j

%0 Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD = ((S - D) /[(S+D)/2]} x 100 number (M g/L)

(acceptable range=

10%)

Duplicate D c0.oO'Lq Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number (rag/L)

Blank (should be= < 0.10 mg/L)

L".

o o O~b-CA~

i PtCmC I:- IN LO o13oc)o&uz O%1L-Oq qXq-tA-L**C t,

coin o.oo0o,.t.

bQiO j* 02-(nC'GJ I0

'V 18 [O.VE, to Co I b O.oo3\\I 0qa?*~~

~~

b 3

-- J J I r" -

chIfL5 )o~~~

oc, at e */D j-1icAeSc* r.ee*i

-o. oot.qtz bauj-abit

.O~~

LO.EO0jIO 6):1b2J3.cA hT*A-5Q,-):J Ol IL)]

d,.J 4o

. '.!_._.p___

m ov 4

O 4

&ZJ 3/4 xh 6

L 0

i'lJ "

W:Nte: All samples were andaly~zed~in excess of EPA recommenided hts e bdd

",t- :,*.l-,

5 Labortory ontrlsaia dard:..

.2 Reference standard '

True.value (TV),

,;Measured value (MV)"

J./.RS= MV/TV x 100 n

n r.'.

tm-,.

V-

,' > IK (a c ce p ta b le r a n ge : 9 0 tO 1 1 0 % ) I ge 61, of 98 Reviewed by K64 Date reviewed Q

0 -°I b

Environmental Testing Solutions, Inc.

Page Page 2I of ZL

'........ £..

70)

Iodide reagent:

ACid reagent:

Dt

,analyst [I Date analyzed e-ao Total Residual Chlorine (Orion Electrode Method, Orion 97-Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR2 5 PH/Ion Mett Calibration:

o 0.10 mg/L 1.00 mg/L I

Reference standard number I.-

Note: 'For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number (mg/L)

(mg/L)

(acceptable range = 90 to 110%)

,,ss c o0.5.0 M

9. q. 0 Duplicate sample precision:

Sample Sample ED Sample characteristics Residual chlorine

%RPD = {(S - D) /[(S+D)/2]} x 100 number (mg[L)

(acceptable range 10%)

{)g62t3.o" fOat~cuz--~oo s~q*

O*~

0go~l~,.o W'z. CLV Icir.-s, Itl

!11 IN

.4 Duplicate D -G.003Z3 Sample measurements:

Sample Sample ID Sample characteristics Residual chlorin.e number

/mig/L)

Blank (should be = < 0.10 mg/L)

"7,

,Note: All sampleswere analyz-ed ii'excessof EPA reconimendedholding timne'(15.minutes),uimess"otherwise noted..

.Laborator control sta.. ndard d..

..Reference standard True value (TV)>,:,.

Measured value,,.ý (MV)-

% RS MV /TV x 100

(..(number i(ra L).

gL)-

1,.. '

acceptable range =90 to 110%)

nubr

.0.50<

0_. [EOZ.3 O

I..

6

~ag 62 of 98 Review ed by I R Z Date reviewed 1 OZ-l-*6

11 Mnmenta.Iiesting Saltohjns,;. ~

Page q

Analyst L:.

Date analyzed Titj-,nt normality andi,ultiniier determination:

Alkalinity (SM 2320 B) 4atrix: Water, RL 1.0 mg CaCOJL

.. Titrate samples to pH 4.50 S.U.

PaTgeted [ ;

>6fy;[

Timne mitiiited l~C

-Time-cornoj ted Deionized Titrant check Begin End Total (5 ml NaZCO3 x 0.05)/E.

(Nx 50000)/100 ml sample water reference standard ml ml ml

= 0.25/E

.Nx 500

=4.5 S.U.

number number (E)

(acceptable range = 0.0180 - 0.0220) 5-5; 0.3 lop3 T,.

0,O0

-. iin:

Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS = MV / TV x 100 number (TV) volume Begin.

End Total Multiplier (mag CaCO3fL)

(acceptable range (mg CaCO3/L)

(ml) ml ml ml

=90 to 110%)

S3s 100 100 oz3 10.0 TLC r

"lc4 Duplicate sampie precision:.

Sampl.

"Sample Alkalinity

% IRPD Sample.

Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S - D) /[(S+D)/21} x 100 number (ml) ml ml

. ml (acceptable range=+ 10%)

02-o~~~~

M

~

,U3 14 2114 D u p lic a te (B )

J -

I A S -

1 4 1 L e l -.

D I Q U q 'O Matrix spike recove r:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (i(mg CaCOIL)

(mg CaCO3/L)

(ml) ml ml ml 50 IO Ic.3&q.3

.o 1

0 L.-+

Sample alkalinity (B)

Measured spike value (MV)

% R = MV / SV x 100 (Mrg CaCO3/L)

MV = A - B (acceptable range (Mg CaCO3/L)

= 75 to 125%)

Sample measurements:

SSample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3 L)

CAD

-0 U %,Sx

) i3/4 o.__

._.. v4lo i

31;:.:

76-o

-o-.

34.(,

'q-.(

k~o J b

-oI4 Vo.

3,3~ (P A*.,

c,_

S1i

-i-

-)

-0 U

.l Ilage 63 of Reviewed by:

LI~IZ1 Date reviewed: I Cx -OTR7

Page iO Pagej of Analyst !

F_6_I Date' analyzed {-0,-o,:jLj-Alkalinity

'(SM2320 B)

Matrix: Water, RL 1.0 mg CaCO3 /L Titrate samples to pH 1=4.50 S.U.

Time initiated.7 Time c. mrnplete d pH of Normality maLt (A) of H 2S(4 pH Factor or Multiplier Deionized Titrant check Begin End Total

= (5 ml Na 2 3

Ni 50000)/10 mi-sample water reference

..standard ml ml ml 0.251E 500

= 4.5 S.U.

number number (E)

(acceptable range = 0.0180 - 0.0220)

Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS =MV/TV x 100 number (TV) volume Begin End Total Multiplier (Ing CaCO3fL)

(acceptable range (Mg CaCO 3/L)

(ml) ml ml ml

= 90 to 110%)

17v-J'-s te1-ý 100 100

.9-4

q. q (cP.4 9o5 t>.

Duplicate sam le precision:

Sample Alkalinity

%RPD Sample Sample ID

. volume Begin End Total Multiplier (rmg CaCO3/L)

(S - D)/j(S+D)/21} x 100 number (ml) ml ml ml (acceptable range--__ 10%)

~D Duplicate (B) 9.

4, q.3

.0 10 Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (Mg CaCO3 /L)

(Mg CaCO3/L)

(ml) ml ml ml i~-sLet rSo 1".

Sample alkalinity (B)

Measured spike value (MV)

% R MV I SV x 100 (Mrg CaCOA/L)

MV = A - B (acceptable range (mg CaCO3IL)

= 75 to 125%

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3(L) 24 ~o

'-3

.~1

'0r o*.olC). S.

£.-""4-,

S.

M0_ýO

ýUA 5ý11.c5D~~

4.C 1t3020-.o 0,

_%1-jj-j 341.

5.1

__S

__2.__

Page 64 Reviewed by:

F Datereviewed: 1 02_-O,?-o

Environmental Tfsting Solutions, Inc.

.Page II Page I

of Analyst [

L*L Date analyzed ty:

Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO3 /L Titrate samples to PH = 4.50 S.U.

T

..e

,cmp.etd i"Tim e~iii6

bO Tine cofinpleted Lg*

Tiftant normality rind multinJh~r dr~tpminatinn~

T..

pH of Normality Normality (M) of H 2 SO 4 pH Fact6r or Multiplier Deionized Titrant check Begin End Total

= (5 ml Na 2CO 3 x 0.05)/E

= (Nx 50000)/ 100 ml sample water reference standard ml ml ml

=0.25/E

=Nx 500 4.5 S.U.

number number (E)

(acceptable range = 0.0180 - 0.0220)

,4.G*

SN2.-+I

)rca5U6'5 o.1 1,2.o WlI t.o~lO 1o.

W-

-) --

? 0.

Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3 L)

(acceptable range (mg CaCO3IL)

(ml) ml

ml ml

=90to110%)

i,N55[O*.

100 100 Qi0.o q4.

c,4 Io_5 qq q°-

Duplicate sam ple precision:

Sample Alkalinity

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S - D) /[(S+D)/2]} x 100 number (ml) ml ml ml (acceptable range=+/- 10%)

02 0 s O a IY1-~

bC) t2)*4 A,5 (p, 1 O

_-10-_L?

Duplicate (B)

D

(.0 D

Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mg CaCO31L)

(ml) ml ml ml Iss b 50 1o DO "5.5 -9S,o 0

t.

o.

Sample alkalinity (B)

Measured spike value (MV)

% R MV I SV x 100 (msi CaCO3/L)

MV = A - B (acceptable range (mg CaCO3/L)

-75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOAL)

Q2--5.LX.i

.d-* 1D Moo ko 0.j 7 z.

6

_4 4

ýL ý -

L__

o A2-.0 3 -c'3 fb.

.1

.i 1 A

2-0 3-1

)1o 8O 5

-,0211.

1

-V___-._____

323 k

5.

I 01i

-0 4 Grncog'L*

,o

-39

., 1 Page 65 of 98 Reviewed by:

D rvw:"

i Date reviewed: 102-- IS 1

a Page 1-Page*

of 3

D Environm,,,tal Testing solutions, Inc, Analyst eS

,Date analyzed Ca - 5.0C Alkalinity (SM 2320 B)

Matrix: 'Water, RL - 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.

Time iniiiated Time comnpleted Titrant normality and multiplier determination:

"". ormait Normality (.HSO 4

pH Factor or Multiplier Deionized Titrant check Begin End Total

= (5 a2 "50

/100 ml sample water reference standard ml ml ml 25/E V*x500

=45 S.U-.number number

.(E)

(acceptable range = 0.0180 - 0.0220)

Laboratory control standard:

Reference standard True value Sample Alkalinity (MV)

% RS MV TV x 100 number*

(TV) volume Begin End Total Multiplier (rag CaCO3/L) iacceptable range (Mg CaCO 31L)

(ml) ml ml ml

= 90 to 110%)

(

t loo100 100 3q, cjz l&-

,"T, Duplicate sam le precision:

Sample Alkalinity

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCOJL)

{(S - D) /1(S+D)/2]} x 100 number (ml) ml ml ml (acceptable range =1 10%)

Duplicate (B)

D Matrix spike recover:."

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCOVL)

(mg CaCO3 [L) ml ml ml 1,135C)~

Co

,to Q

I-l*

I5*

i ZO Sample alkalinity (B)

Measured spike value (MV)

% R = MV SV x 100 (rag CaCO3/L).

MV = A - B (acceptable range (rag CaCO3/L) 75 to 125%)

.Samle measurements:

Sample volume Begin End Total Alkalinity Sample. number Sample ID (ml) ml ml ml Multiplier (mg CaCOjL)

.%Oqb &LsOj A?/**

Is*,-4$.., q.o

)o,.

7 O ozlb.2-.,..... )

10 z,%:z

-62IJ, 5

2 OL 9O 0-209.61l-,' 17

__i_

TL-.s

.o

_1

/3_343____

i66 of 98 Reviewed by:

D r

"vee1d-2 Date reviewed: I O-Z -I

"ETS e

nvn~me tale l'~luln.Ic Page lŽ Page 3 of Z3 Analystt77C Date-analyzed Tn'.,.,f,nrmna if, n,i~r mcjliinlipr determinatioun Alkalinity (SM 2320 B)

Matrix: Water, RL = 1.0 mg CaCO 3/L Titrate samples to pH-4.50 S.U.

Time initiated Time completed pH of Normality Normality (IV) of H2SO4 pH Factor or Multiplier Deioniz--

Titrant check tR en

=

ml Na2CO3 x 0.05)/E

= (N x 50000)/ 100 ml sample water reference standard ml ml ml

=Nx500

= 4.5 S.U.

number number (E)

(acceptable range = 0.0180 - 0.0220)

Laboratory control standard:

Reference~standard True value Sample Alkalinity (MV)

% RS = MV / TV x 100 number (TV) 1 volume Begin End Total Multiplier (mg CaCO,/L)

(acceptable range (mg CaCO3/L)

(mi)

MI ml ml

=90 to 110%)

IMSSU57q 100 100

4.3 434 q.9 o.5 ql qtoo Duplicate sam le precision

Sample Alkalinity

%RP=

Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L)

((S -D) /[(S+D)/2)} x 100 number

."TPA (mi) ml ml ml (acceptable range=+ 10%)

2-Y I~\\) 100 A44-4 le-V (1___

4 Duplicate (B)

D Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCQ3/L)

(Mg CaCO/L)

(mi) mi Ml ml Sample alkalinity (B)

Measured spike value (MV)

% R = MV / SV x 100 (mng CalCa/L)

MV = A - B (acceptable range (Ing CaCO3/L)

= 75 to 125%)

h

.C1 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml-Multiplier (Mg CaCO3 L)

SqO2/.Z.2 0

Cr OP~IQ 12.1 C-2_

l 1'bJ.'

1.

311A110

-- I too i

aJC

.Oz-to.oJc

kAl-s&c IoQ fl,*

,8 t age 67 of 9N Reviewed by:

I V-IIIZ Date reviewed:

0.s-uci-o ]

-ET Page 4-+

Page of ;t Total Hardness (SM 2340 C)

RL'= 1.0 mg CaCO3/L Analyst [-£,,

"7j Date analyzed OýR. 0,6.D 0 Titrant normality and multiplier determination:

Time initiated [Dt Time completed i i 15-*,

rTitrant '

Normality'check"

'Begin End

- Total Normality (N) of EDTA pH Factor-or-Multiplier reference standard ml ml ml

0.2[E

(N x 50000)/50 ml sample number number mtE (acceptable range = 0.0180 - 0.0220)

= N x 1000 Nf-3c/* ThSS 0.0 10.0D 10.0 o.0ae O.o Laboratory control standard:.

Reference standard True value Sample

(

'aHardness (MV)r%

RS = M TV x 100 number (TV)

I volume Begin Ed Total Multiplier (g(aeptable range (bg CaCO(JL)

(mlu ml ml Iml

= 90 to 110%)

40 5

o.o 0.o "O.O '

I oo T

Duplicate sam le precision:

rT.o~rsXof Col[',vcg a

St.,

1

Sample lHardness

%/RPD=

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S - D) /[(S+D)/21) x I100.

number

_(ml) ml ml ml OZC6-o,4, M t~S S4_

I o

C7.o

,7.-

Q0.0 s

100 Duplicate(B)

D lM-10

"-I'-

Matrix spike recover:,

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(Mg CaCO 3/L)

(ml) ml ml ml Tms3s I

-4D 10O cR&OICgi o?.O 3

Sample hardness (B)

Measured spike value (MV)

% R MV SV x 100 (mg caCo 3/L)

MV = A - B (acceptable range (mg CaCO3IL) 75 to 125%)

Samplemeasurements:.

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC0 31L)

TV = ND Blank 50 5 D" t*.t (should be =omgCaCO3/L) 49>es,,

IM.O

R.0 DCo 0

N

, CO616.0 A5

,+.S "

f0l1-tYý,iý fl H

'5 4.

+5 9b o __________z" j;.q Iq."-: 5.3 '

I age 68 1-f I

r Ft-fnt iqtrnt i

q.r..d n*Anle must be diluted.

Reviewedby:

by:-

DDate reviewed 1 02_o6,0q---]

0

.9-Enironment al Testng Solutionsý Inc.

Page,

Page c9, of A

Total Hardness (SM 2340 C)

RLE= 1.0 ng CaCO 3/L Analyst Date analyzed OA, Time initiated F717 Time completed and multiplier determination:

Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)

(mg CaCO3VL)

(ml) ml ml ml t'ýLe,'-Q40o 5*o d4.I o',-4 4,3 co.?(

Sample hardness (B)

Measured spike value (MV)

% R MV / SV x 100 (Mg CaCO3fL)

MV = A - B (acceptable range (mg CaCOfL) 75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)

TV+FD la1nk TV =+4B (should be 0 mg CaC0 3/L)

'-T

,C)--

i-iAýcJ 10 0.0 O~~a~o

~)00 110 93 5

I b1O2~.V) rbi-xxttI I

~ ~

~414 6 ~T N9ote f 91m1 o irn suesml utb iue.Rvee y

aervee l~)5&

Page Note: If >1 5ml of titrant is used, sample must be diluted.

Reviewed by: 1 6 LY--ý.

I Datereviewed I (12-063-C)Ci I

ET5

-0 v~*mna o*tn ou o

,h*

Page L-4 Page._

of 3..

Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO 3/L Analyst Date analyzed Co.-

1 Time initiated [II*

llI]

Time completed ij-1 i o

Titrant normaliy and multiplier determination:

Titiant Normmalitycheck reference standard number number Begin Sml End ml Total

ýml Normality (N) of EDTA

=0.2/E (acceptable range = 0.0180- 0.0220) pH Factor or Multiplier

= (Nx 50000)150 ml sample:

= N x 1000 I:r"z30 6,-, 1 o

)

1.

0

l. U I

.o.o Laboratory control standard:

Reference standard True value Sample Hardness (MV)

% RS =MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (Ing CaCO3/L)

(ml) ml ml ml

= 90 to 110%)

lS5 LcL-5l (

40

.50 A).o D,o 0

.oo Duplicate sam le precision:

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

.{(S - D) /[(S+D)/2])

100 number (ml) ml ml ml Oz-o~Y~~

~50 IýO Iii Sj %~~~Q)

Duplicate (B) 7 D

j4.i4 D4 Matrix spike recovery: Mr(45 C'A-tA Reference standard, Spike value Sample Spike hardness (A) number (SV) volume Begin End.

Total Multiplier (mg CaCO3/L)

(mg CaCO3 /L)

(ml) ml ml ml 0_*0_*__

(.o 4 c'1.0 13D Sample hardness (B)

Measured spike value (MV)

% R MV / SV x 100 (ing CaCO3/L)

MV = A - B (acceptable range (Ing CaCOIL)

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml.

ml ml Multiplier (Mlg CaCO 3/L)

TV=ND Blank (should be 0 mg CaCO3/L) 50 2I l-.

1.

0 0 Z- -I?) Oi Ff2{

I.~~

(P_

__4___

'02-"4o

". Z

.*4

§ 4.5

...oz-ioDt,,(.,

J

_-3<*:....- *

.o.*-.5 4.5

"_q 06z21i fJ P'4 Po*&**'

I

,S 4*5

.o 3.1 I

-zo.---2, 1

i4

-Zo~

.i, I

) )-

+

IPage I 0Pae 70 Note: If >ISml of titrant is used, sample must be diluted.

Reviewed by:

j*Lv-Date reviewed 02

'env nmnmentau Testing Solutions, In.

Page 5

Page of 3 Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Analyst Zc ]

Date analyzed jA -

j Time initiated Time completed Titrant normality and multiplier, determination:

Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier referene-.

- t"ýdard ml ml ml 0.2/1E

=(N x 50000)/ 50 ml sample number number

_(A (acceptable range = 0.0180 -*0.0220)

=Nx 1000 Laboratory control standard:.

Reference standard True value Sample" Hardness (MV)

% RS =MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L)

(acceptable range (mg CaCO3/L)

(ml) ml ml ml

= 90 to 110%)

40 50 c:toO 4A Io0tO Duplicate sam ple precision:-

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (Mg CaCO31L)

{(S - D) /[(S+D)/2]} x 100 number (ml) ml ml ml

)9020S.0) fIA-SPOt

-R it r-+ 15.

'4.

20,C)

_1040 Duplicate (B) 4 2-D Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier

.(mg CaCO3'L)

(mg CaCO3fL)

(ml) ml ml ml 4T,.-s0 o L5 1O l.I l-l.1 f0.0 120 Sample hardness (B)

Measured spike value (MV)

% R = MV SV x 100 (amg CaCO3/L)

MV = A - B (acceptable range (mg CaCO3/L) 75 to 125%)

q

3 to Clo et ° Sample measurements:

Sample volume Begin End Total Hardness*

Sample number Sample ID (ml) ml ml ml Multiplier (Mg CaCO3/L)

TV = ND Blank (shlouidb 6102_11. Iq iA_-c

, o 1

__.__.o 01 OZ 13.'

15 3,*--- -> "

60H5 q:

(

01..01 I-F*.2-

),..

eowo'Os.o.

WS

_'8 O-107 '.o 0q VA ojjr i

ICL)

-S~ '+V__

Page 71 gotef:

>15ml of titrant is used, sample must be diluted.

Reviewed by: [iIZ

-.-- I Date reviewed I 0Z-I-Ti, 7]

4 Envinon mental Testing Solut Ion, Inc.

Page 51 Page 3

of 3 Total Hardness (SM 2340 C)

RL = 1.0 mg CaCO3/L Analyst *

"1 "

Date analyzed L0,),- 5 0 i

Time Initiated ---

Time completed LIZ

[

iTitrant normality and multiplier determination:

"I Titrant Normalitycheck Begin End Total Normality (I) of EDTA referermee*-.-

-tandard ml mi ml

= 0.2/E number number (Ri (blc m ane- = A 0180- 0.0220) pH Factor or Multiplier

= (N x 50000)/50 ml sample

= Nv 1f000 Laboratory control standard:

Reference standard True value Sample Hardness (MV)

% RS = MV /TV x 100 number (TV).

volume Begin End Total Multiplier (Mg CaCO3fL)

(acceptable range (mg CaCOJfL)

(ml) ml ml ml

= 90 to 110%)

r 3s tsw 40 50 R________-___.__,b_

Q__________

Duplicate sample precision:

Sample Hardness

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L)

{(S - D) /[(S+D)/21} x 100 number "f4 (ml) ml ml ml mzi.5*35 UT LA.)A 5D 14A1 9' 1

4.

L'o.O s S

Duplicate (B)

'J" -

2

},*!

D 3 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)

I (mg CaCO3/L)

(ml) ml ml ml INI!e4Q1 L

1 1,5 1c.7-s4 LP -Z I~qo Ico Sample hardness (B)

Measured spike value (MV)

% R MV / SV x 100 (S g CaCOmL)

MV = A - B (acceptable range (mg CaCO3 L)

= 75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOVL)

TV = ND Blank I

-- shouid be =0 Mg CaCG3/L,)

-tA b213,ol li~tQ-

,)S.4,,

4.

.it.O 6 1 )

71 Z -.

b.90(,!5.

6t~~-zoL D

lo i

I Page 72 Oo If >15,i of titrant is used, sample must be diluted.

Reviewed by: [

jj Date reviewed [62

-1,5-61

Sequoyah Nuclear Plant Biomonitoring February 10 - 17, 2009 Appendix D Reference Toxicant Test and Control Chart Page 73 of 98

0 0R Pimephales promelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.

Environmental Testing Solutions, Inc.

1 9 I

Ix I

I

-I I

I I

I i

I I

I.

I USEPA Control Limits (+2 Standard Deviations) 1.0 0.8 0.6 0.4 I

I I

I i

I I

1 9 1 2 I

I I

I

(

1 a

I I

i USEPA Warning and Control Limits (75h and 90t Percentile CVs)

I CU 1.0 [

0.8 I 0.6 F

......I

........I I

0.4 1.2 I

I I

I Laboratory Warning and Control Limits (10th and 25 h Percentile CVs) 1.0 0.8 0.6 S............

0.4 I

I I

T I

I I

dIe

.. *T est d ate 7-day IC25 =25% inhibition concentration. An estimation of-the concentration of potassium chloride "

that would cause a 25% reduction in Pimephales growth for the test population.

- Central Tendency (mean IC2 )

Warning Limits (mean IC25 +/- SALO or SA75)

Control Limits (mean IC25 +/- SA.25, SA.90, or 2 Standard Deviations)

Page 74 of 98

SET Environmental Testing Solutions, Inc.

Test number Test date 7-day IC2

" CT I ' (g/L KCI)

(g/L KCI)

Pimephales promelas Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory S

Control Limits S.,

Warning Limits SA.2s Control Limits SA.75 CT - 2S CT + 2S CT - SA.10 CT + SA.1o CT-SA.2 CT + SA.25 USEPA Warning Limits SA.

CT - SA.75 CT + SA.7s USEPA Control Limits.7 CT - S,_,

CT + s.,

CV 1

2 3

4 5

6 7

8 9

10 II 12 13 14 15 16 17 18 19 20 04-15-08 0.60 05-06-08 0.62 05-20-08 0.66 06-03-08 0.64 06-10-08 0.76 08-05-08 0.69' 08-12-08 0.76 09-09-08 0.79 09-11-08 0.59 10-07-08 0.5T 10-14-08 0.63 10-28-08 0.79 11-04-08 0.67 12-09-08 0.69 01-06-09 0.76 01-13-09 0.82 01-15-09 0.77 01-21-09 0.78.

02-03-09 0.79 02-10-09 0.83 0.61 0.63 0.63 0.65 0.66 0.67 0.69 0.68 0.67 0.66 0.67 0.67 0.68 0.68 0.69 0.69 0.70 0.70 0.71 0.01 0.59 0.63 0.07 0.54 0.03 0.57 0.68 0.08 0.55 0.02 0.58 0.68 0.08 0.55 0.06 0.53 0.78 0.08 0.58 0.06 0.55 0.77 0.08 0.58 0.06 0.55 0.80 0.08 0.59 0.07 0.55 0.83 0.08 0.61 0.08 0.53 0.83 0.08

' 0.60 0.08 0.51 0.82 0.08 0.59 0.07 0.51 0.81 0.08 0.58 0.08 0.52 0.83 0,08 0.59 0.08 0.52 0.83 0.08.

0.59 0.07 0.53 0.82 0.08 0.59 0.07 0.53 0.83 0.08 0.60 0.08.

0.53 0.85 0.08 0.61 0.08 0.54 0.85 0.08 0.61 0.08 "0.54 0.86 0.08 0.62 0.08 0.54 0.86 0.08 0.62 0.08 0.55 0.88 0.09 0.62 0.68 0.13 0.48 0.74 0.70 0.13 0.49 0.76 0.70 0.13 0.50 0.76 0.73 0.14 0.52 0.79 0.74 0.14 0.52 0.80 0.75 0.14 0.53 0.82 0.77 0.14 0.54 0.83 0.76 0.14 0.54 0.82 0.75 0.14 0.53 0.81 0.74 0.14 0.52 0.80 0.76 0.14 0.53 0.82 0.76 0.14 0.53 0.82 0.76 0.14 0.53 0.82 0.76 0.14 0.54 0.82 0.77 0.14 0.54 0.83 0.78 0.15 0.55 0.84 0.78 0.15 0.55 0.85 0.79 0.15 0.56 0.85 0.80 0.15 0.56 0.86 0.23 0.24 0.24 0,25 0.25 0.26 0.26 0.26 0.25 0.25 0.26 0.26 0.26 0.26 0.26 0.26 0.27 0.27 0.27 0.38 0.39 0.39 0.41 0.41 0.42.

0.43 0.42 0.41 0.41 0.42 0.42 0.42 0.42 0.43 0.43 0.43 0.44 0.44 0.84 0.27 0.34 0.86 0.28 0.34 0.87 0.28 0.35 0.90 0.29 0.36 0.91 0.30 0.36 0.93 0.30 0.37 0.95 031 0.38 0.94 0.30 0.37 0.92 0.30 0.37 0.92 0.30 0.37 0.93 0.30 0.37 0.93 0.30 0.37 0.93-0.30 0.37 0.94 0.31 0.37 0.95 0.31 _

0.38 0.96 0.31 0.38 0.97 0.31 0.38 0.97 0.32 0.39 0.98 0.32 0.39 0.88 0.01 0.91 0.04 0.91 0.04 0.95 0.09 0.96 0.08 0.98 0.09 1.00 0.10 0.98

. 0.11 0.97 0.12 0.96 0.11 0.98 0.12 0:98 0.11 0.98 0.11 0.99 0.11 1.00 0.11 1.01 0.11 1.01 0.11 1.02 0.11 1.03 0.12 Note:

7-d IC2 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pirnephales growth for the test population.

CT.= Central tendency (mean IC_1).

S = Standard deviation of the IC25 values.

Laboratory Control and Warning Limits Laboratory control and.warning limits were established using the standard deviation of the tC25 values corresponding to the 10th and 25th percentile CVs.

These ranges are more stringenl than the control and warning limits recommended by USEPA for the test method and endpoint.

S,10 = Standard deviation corresponding to the 10a percentile CV. (S& o= 0.12)

S&25= Standard deviation corresponding to the 2 5 h percentile CV. (S&25 = 0.21)

USEPA Control and Warning Limits S.75 Standard deviation corresponding to the 7 5th percentile CV. (SA.7 - 0.38)

S,,_,§ Standard deviation corresponding to the 90th percentile CV. (SA.S9,= 0.45)

CV = Coefficient of variation of the IC25 values.

USEPA_ 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimtination Program. EPA-833-R-O0-003. US Envirotmental Protection Agency, Cincitntti, OR.

Orgarmsms obtainedfrats Aqualox, Inc.

atox02-10-09.

TPrecision of Endpoint Measurements Pimeph ales promelas Chronic Reference Toxicant Data Environmental Testing Solutions, Inc.

Test number Test date Control Survival Control Mean Growth CT Cv CT MSD PMSD CT for Control Growth

(%)

(g/larvae)

(mgarvae)

(%)

for Control Growth CV (%)

(%)

for PMSD (%)

1 2

3 4

5 6

7 8

9 10 11 12 13 14 15 16, 17 18 19 20 04-15-08 05-06-08 05-20-08 06-03-08 06-10-08 08-05-08 08-12-08 09-09-08 09-11-08 10-07-08 10-14-08 10-28-08 11-04-08 12-09-08 01-06-09 01-13-09 01-15-09 01-21-09 02-03-09 02-10-09 100 100 100 100 97.5 100 100

.100 100 100 100 100 97.5 100 100 97.5 100 97.5 100 100 0.898 0.857 0.844 0.918 0.724 0.854 0.674 0.710 0.824 0.788 0.740 0.586

.0.643 0.522 0.644 0.556 0.707 0.594 0.671 0.591 0.877 0.866 0.879 0.848 0.849 0.824 0.810 0.811 0.809 0.803 0.784 0.774 0.756 0.748 0.736 0.734 0.727 0.724 0.717 9.1 4.4 19.2 6.1 10.9 15.3 5.3 11.1 11.1 1.8 3.2 6.5 2.5 2.4 3,2 9.7 6.5 4.9

.8.4 5A1 6.7 10.9 9.7 9.9 10.8 10.0 10.2 10.3 9.4 8.9 8.7 8.2 7.8 7.5 7.6 7.5 7.4 7.4 7.3 0.08 0.16 0.16 0.09 0.11 0.13 0.07 0.12 0.11 0.10 0.05 0.10 0.12 0.05 0.09 0.06 0.09 0.07 0.06 0.09 9.1 18.2 18.8 9.4 15.7 15.0 9.8 17.3 12.9 12.9 7.2 17.8 18.7 10.0 13.3 11.2 13.3 11.5 9.6 15.7 13.7 15.4 13.9 14.3 14.4 13.7 14,2 14.0 13.9 13.3 13.7 14.1 13.8" 13.7 13.6 13.6 13.5 13.3 13.4 Note:

CV = Coefficient of variation for control growth.

Lower CV bound determined by USEPA (10th percentile) = 3.5%.

Upper CV bound determined by USEPA (90 th percentile) = 20%

MSD Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10i percentile) = 12%.

Upper PMSD bound determined by USEPA (9 0' percentile) 30%..

CT = Central Tendancy (mean Control Growth, CV, or PMSD)

USEPA. ý,2000. Understanding and Acco'unting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program..EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2,0la,2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005, US Environmental Protection Agency, Cincinnati, OH.

Organisms obtained from Aquatox, Inc.

atox02-10-09 Page 76 of 98

0 U

o 6

j-SoJ Pimephales promelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements Organism Source: Aquatox, Inc.

=....... -

Environmental Testing Solutions, Inc.

1L25 I

I j

I

, I I

I II I i I I

I 1.00 0.75 0.50 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)-

I I

I o

20 U

30 20 10 0

30 20 10 n

U I B

(

percentile I

I30

~USEPA Upper PMSD Bound (90t* percentile < 30.0%)

-~ I I

I I I I

I I

II I

I I

I '

I I

I Test date i -i---.-

C*ontrol Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the-minimum significant difference between the control and treatment that can be declared statistically significant.,

Central Tendency (mean Control Growth, CV, or PMSD)

Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) a*ge 77 0if98

c Envntopmta tI atting Solution 5, Inc.

Page I of 5

.,.,.L

gl" Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0).

Species: Pinephalespromelas PpKCICR Test Number:

1 10O Dilution preparation information:

Comments:

KCI CHM number:

CVtý L402..-

Stock preparation:

50 g KCI/L: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mg/L) 450 600 750 900 1050 Stock volume (mL-)

9 12 15 18 21 Diluent volume (mL) 991 988 985 982 9791 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:

Test information:

Organism age:

tso,

O i*uv& O..:b Randomizing template: "

uý.L-P-Date and times organisms O-L-O*1-0 0t B0o0 Incubator number and were born between:

shelf location:

Organism source:

A-TOY, 6*#TA ?P 02-CA-.

Artemia lot number:

____Iq__

Transfer bowl information:

pH =

SU Temperature

'C Total drying time:

"+Ito j4, q.32.

Date Time in: oz-I'l.]c 0

L1 Average.transfer volume:

0.,1 q52 S'--

Date /Time out: &I. I 1n11 Oven temperature:

C Daily feeding and renewal information:

Day Date Morning Afternoon Test initiation, MIHS Analyst feeding feeding time renewal, or batch used time termination time 0

02-1o-0o

-Q4 zo&oQq I~

I Ca._ ra -

~

cA ccit-1(

o~

3 0-t-130-o 0oo iqco.

4 OL-1,4-Oct 0*O

30)

Co 10"61 A.'~P a?. -I£.s*

oo*

qb*

. _______5 o 6 O*31-c Co tAot o

t-t o

K 4 C formation:

Acceptance criteria Summary of test endpoints:

I o -

I

. -20%

7-dav'LCzn C14 3,O

..weight per inital larvae:

-weight per surviving larvae:

i 4.~

1 '0.99 !

KRI SH NOEC I A.Sl I

> 0.25 mg/larvae LOEC II v I U

4 w ---

ChV IC 5 r1 0o

E-nuironmental TeOing Solutions, InC.

Page 2 of 5 jl~

Species: Pimeph ales promelas PpKC1CR Test Number:'

1M Survival and Growth Data Day Control 450 mr KCI/L

-600 mg KCI/L A

B C

D E

F G

H I

J K

L*

0 b0 10 1/D C

"0 to

/0 10

/0

/0 2o I0 tO 0

13 10 t0 I0 1O 1 '0 IC/

0 to to

/o I0 to t /03 *6 13 0

/0

/

4 ito to.

tO I

to) 10 O

o b

10 to 10 5

So (0

/to I r (0

)0 10b/0 D

/0 6

0-

/,/0

/0 10

/0 C) o*

!0

/

o

/0

/0 7

VSK (0/0

/to (0

6 to to to I0 (0 (0

A = Pan weight (mg)

Tray color code:: Y.* A9 Analyst: L 3t.09105-03 1A 3.19 14-..l Iqk.Mo 18.%3 MAL 13-1.1 ýq.1 q 13.q' tqj Date: c(7--0 -O_

B = Pan + Larvae weight Analyst;:

____4' p1-15

11.

lt,,A Z2 18.46 o

Date:

b.

o-,-IS--q C = Larvae weight (mg)

=A-B

t

.Zb i*

q.40 S.Rz7-q,6,5 6.oq S.qZ t..61 S,tG t

<.,.gO Weight per initial number of larvae (mg)

= C / Initial number of larvae 0"

IV

'aa O° 0

P ZZ11V 0*

Jvy 10V

%-0 0

Wa 0*

,Ave ra~ge Percent weight per reduction initial from control 0o I

I number of

(%)

larvae (mrg)

=,

I S

4 S C 5 4 5i

'.*,4 o.Sý'

r 40171 Comment codes:..c,= clear, d = dead,.fg-.fingusj k = killed, m missing, sk = sick, sm= unusually small, Ig = unusually' large, d&r decanted and returned, w = wounded.

"3:f*

r~.ua M e 98 RT ge 79 of 98 Calculations and data-reviewed:J Comments:

TRW

1,ET "v Eý.vj'r.ý-.

lqftln~g Solutio-, In-Page 3 of 5 A.

PpKCICR Test Number:

I'O Species: Pimephales promelas Survival and Growth Data Day 750 m KCI/L 900 m KCI/L

.1050 m KCI/L M

N

.0 P

Q R

S T

U V

ý W'

.X 0

ID 1

/D 10 UQ

/

/0 10

/0

/c 0 A0

/0-s'k 5O -1 Lk 2

4d.

tc i0 tO 3__ _

/o to /0 o

i C 1

L L

S 4

6it:

O0 5

1 C) to 10 LAo SA q

3 3

6 S I

-a I'

10 (0

(0 6

1

7.

3

-1 6

f*to 10 (0 -7' ~

s' 1~t

.-o 7

a l)-

10 to t3 5

3 A

Pan weight (mg)

Tray color code:: reA 13 up Analyst:

L.,Y

-t Nl ?

q i 3.F lt.cia N.Otp 1q.13 IS.SI 13.3st NO 11.3o 1+1S Date:

67 =I3-10-0 B = Pan + Larvae weight Analyst:

LA R

It~q M

S I. I. 13 q,40 17-34 MO.A I IAoT}

1., T-Iq'3

.tI.

Date :

to

1. 1 - 0 C = Larvae weight (mg)

Weight per initial number oflarvae (mg)

C /Initial number of larvae 15ý

-V Aver weig initi.

rage Percent

ht per reduction al from control aber of

(%)

ae (me) 870."O U9..

o.Sq 3,7.

0. nol 61 -RI..,

.1 a.~i h

Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Jg.*.unusually large, d&r = decantedand r'eturned, w wounded. "

Calculations and data revie'wed: 4

Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.

Test number:

. PpKCICR #170 Test dates:

February 10-17, 2009 Reveiwed-by:

Coacentta6na (mglL Replicatse hdal number of

.Riaalsomberof A=r.a,,eigbt(n9 a= Pa +aruv rae Lsweighu t(mg)

WeightlSorviviag Mea wýigbtl CofficieotfvariaWlad i

Weight/ Iitial naumber Meansurylval Meaiaaght/luitial Coefficient of Peeent redvctio from KO) larvae larvae weight (mg)

-A-B u..m.. vae(ng)

$o i'vrguaamberat to.wM',gu.

otlarwe-m g)

(%1.

.n.bfr of larvae variation 1%)

control 1%)

larvae (rg) i

(%)

(ng)

A 10 10 14,09 19.58 5.49 0,549 0.549 Control B

10 10 15.03 21.15 6.12 0.612 0.591 5.1 0.612 100.0 0.591 5.1 Not applicable C

10

-10 13.94 19.85 5.91 0.591 0.591 D_"___"

10 10 13.79 19.92 6.13 0.613 0.613 E

10 10 14.26.

19.16 4.90 0.490.

0.490 F

10 10 14.46 20.28 5.82 0.582 0.548 99 0.582 97.5 0.535 12.9 9.6 G

10 9

13.83 18.46 4.63 0.514

.90.463 10 10 14.12 20.16 6.04 0.604 0.604 10 10 13.87 19.29 5.42 0.542 0.542 6J 10 10 14.34 20.36 6.02 0.602

.0.602 K

10 10 13.49 19.09 5.60 0.560 0.560 L

10 10 14.19 19.69 5.50 0.550 0.550 M

10 8

13.44 17.44 4.00 0.500 0.400 750 N

10 10 14.14 19.87 5.73 0.573 0.545 6.5 0.573 95.0 0.520 15.8 12.1 0

10 10 13.86 19.18 5.32 0.532 0.532 F

10 10 13.46 19.20 5.74 0.574 0.574 Q

10 7

14.98 19.11 4.13 0.590 0.413, R

10 5

14.06 17.34 3.28 0.6W6 0.328.

S 10 6

14.93 19.02 4.09 0.682 0.409 T

10 6

15.51 18.95 3.44 0.573 0.344 U

10 2

13.36 14.53 1.17 0.585 0.117 1050 V

10 3

14.08 15.89 1.81 0.603 0.645 9.4 0.181 27.5 0.179 24.3 69.8 W

10 3

12.30 14.44 2.14 0.713 0.214 X

10.

3 14.75 16.78 2.03 0.677 0.203 Donnett's ISD v'alue:

PMSD:

,0.0926

'15.7 MSD =

Minimum Significant Difference PMSD Percent Minimunm Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatnent that can be declared statistically significant in a. whole effl.uent toxicity test.

Lower PMSD bound determined by USEPA (l0th percentile) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined froom the loth and 90th percentile, respectively, of PMSD data from EPA's WET lnterlaboratoty 'Vaiibility Study (USEPA, 2001a; USEPA. 2001b).

USEPA. 2001a, 200lb. Final Rep~rt: lnerlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-14-0I-004 and EPA-821-B-O1-005. US Environmental Protection Agency, Cincinnati, OH.

Organisms obtained from Aquatox. Inc.

atox02-104)9

Statistical Analyses Envirannient*al Testing Solutions, Inc.

Larval Fish Growth and Survival Test-7 Day Survival Start Date: `:.21j0/2009.

Test ID:

PpKCICR Sample ID:

REF-Ref Toxicant End Date:

1'2/17/2009 Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

KCL-Potassium chloride Sample Date:

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

PP-Pimephales promelas Comments:

Conc-mg/L 1

2 3

4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 0.9000 1.0000 600 1.0000 1.0000 1.0000 1.0000 750 0.8000 1.0000 1.0000 1.0000 900 0.7000 0.5000 0.6000 0.6000 1050

.. 0.2000 0.3000 0.3000 0.3000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/]L Mean N-Mean Mean Min Max CV%

N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4

0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942

4.

16.00 10.00 1

40 600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4

18.00 10.00 0

40 750.

0.9500 0.9500 1.3358 1.1071 1.4120 11.411 4

16.00

10.00 2

40

900 0.6000 0.6000 0.8872 0.7854 0.9912 9.469 4

10.00 J10.00 16 40

1050 0.2750 0.2750 0.5506 0.4636 0.5796 10.532 4

10.00 10.00 29 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <

0.01) 0.83137703 0.884

-1.5887618 3.41097967 Equality of variancecannot be confirmed Hypothesis Test (1-tail,,0.05)

NOEC LOEC ChV TU Steel's Many-OneRank Test 750 900 821.583836 Treatments vs D-Control Trimmed Spearman-Karber Trim Level EC50 95% CL S

0.0% :,,-..

5.0%

10.0%

20.0%

Auto-27.5%

942.95 874.89 1016.31 Organismsl obtained frm.Aquatox, Inc.

4.

V,'[*- ;

N !

atox02-10-09.,

0 n

S4

'EnvironMentalTesting Solutions, Inc.

Statistical Analyses Start'Date:

2/10/2009 EndbDate" 2/17/2009 Sample Date:

Test ID:

PpKCIC Lab ID:

ETS-En Protocol:

FWCIH Larval Fish Growth and Survival Test-7 Day Growth 3R Sample ID:

vir. Testing Sol.

Sample Type:

R-EPA-821-R-02-013 Test Species:

REF-Ref Toxicant.

KCL-Potassium chloride PP-Pimephales promelas Comments:

Conc-mg/L, 1

2 3

4 D-Control

.0.5490 0.6120 0.5910 0.6130 450

" 0.4900 0.5820 0.4630 0.6040

600, 0.5420 0.6020 0.5600 0.5500 750

0.4000 0.5730 0.5320 0.5740 900

'0.4130 0.3280 0.4090 0.3440 1050 0.1170 0.1810 0.2140 0.2030 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max

" CV%

N t-Stat Critical MSD Mean N-Mean D-Control 0.5913 1.0000 0.5913 0.5490 0.6130 5.063 4

0.5913 1.0000 450 0.5348 0.9044 0.5348 0.4630 0.6040 12.856 4

1.397 2.290 0.0926 0.5491 0.9288 600

-0.5635

.0.9531 0.5635

.0.5420 0.6020 4.739 4

0.686 2.290 0.0926 -

0.5491 0.9288 750 0.5198 0.8791 0.5198 0.4000 0.5740 15.815 4

1.767 2.290, 0.0926 0.5198 0.8791 900 0.3735 0.6317 0.3735 0.3280 0.4130 11.733 4

0.3735 0.6317 1050' 0.1788

0.3023 0.1788 0.1170 0.2140 24.276

.4 0.1788 0.3023 Auxiliary Tests' Statistic Critical Skew Kurt Shapiro-Wilk's Test ifidicates normal distribution (p > 0.0 1) 0.94821143 0.844

-.0.8175786 0.4606*2928 Bartleit's Test indicates equal variances (p = 0.21) 4.54906893 11.3448668 Hypothesis Test (1-tail, 0.05)

NOEC LOEC -

ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test.

750

>750 0.09263803 0.15668167 0.0040134 0.00327294 0.34279487 3, 12 Treatmlnts vs D-Control Linear Interpolation (200 Resamples)

Point mg/L SD 95% CL(Exp)

Skew IC05*'

315.80 185.01 86.12 1024.71 0.4865 IC1o 686.81 132.81 139.11 839.84

-1.3149 IC15 767.63 52.55 575.49 832.99

-3.1043 IC20 797.95 33.93 638.23 857.16

-1.6553 IC25.

828.27 28.80 688.64 881.73

-1.5389 IC40 914.44 14.68 866.48 950.31

-0.2463 IC50 959.98 11.87 922.86 990.27

-0.1105 ihdicates IC estriimiae less than the lowest concentration Organisms obtaned from Aquatox, Inc.

atox02-10-09

OýETS, EnvimmýtafTestIngSoludomim Page 4 of 5 Species: Pimeph ales prom elas PpKCICR Test Number:

110 Daily Chemistry:

F" Day

________Analyst F&t P

VL Concentration Parameter CONTROL PH ffiU.

Sot' DO (mL).

Conductivity (prmhos/cm)

Alkalinity (mg CaCO3 L)

Hardness (mg cac 3

)

Temperature (1,C)

-7. ý--? #

,It 1H (S.U.)

'AIO IL717~

-)

I)z ~-1 I

-).~ i-flk DO (mgfL) 450 mg KCI/L Conductivity

([mrhos/cm)

I

)4,6 q1i17

.1 14 Temperature (0C)

'pH (S.U.)

ý*.

S r.'*

3f 4 q l DO (mg/L) 4-3

Ž 600 mg KCI/L Conductivity Temperature¢C)zqI.

q.

z A

-.5 IL.t z.

_0c 7

1 i

1-.1-2!

vH (S.U.)

14, el 0 V q-M, [ ý,O I 7:7-V I --qý al,

0 DO (mg/L)

',5" 1 r4, 0 1

'.4,-4 1 q-6 ji -1 I

750 mg KCI/L Conductivity (u~mh Icm Temperature (0C) 7-4.6A ZA.1 L-4 7%1 I.-

pH (S.U.)

§L~or

?.s' t'4~~IIT~i ~1 1iqhiq S:.-

900 mg KCVL Conductivity lOq 8

1o20 (4nmhos/cm)

Temperature

.___... (0C).

ZAG 2.,"4 7A.1l

2. A q--

2'.,,t.(

0

. } q

-+ :..

+: -!!

1050 mg, Conductivity KCI/L mhos/cm)

Temperature

4. +

STOCK Conductivity nta l

Fana Fnntmhos/cm)ialFinal

,.Initial Fina Initial Final Initial Final L

198 11e-e L06 C -ib 06 4eL-P.

0 0

.*r* Ena4ro mm nal To tn Saou U 0s In, Page 5 of 5 Z,

PpKCICR Test Number:

I_ C)

Species: Pimephales promelas 3

4 5

6

______Analyst LR Concentration Parameter CONTROL lpH (S.U.)

vi 7.1Z 4'1,O i".

-6.

Conductivity

///-

... mhos/cm) 3"*

77--!>

3L[

Alkalinity (mg CaCO3/L)

Hardness (mg CaCO3/L)

CA Temperature pH (S.U.)

.1O 1B-7.

-8.*

9 7

7-B

.qe3 DO (m. L)

,,.3 1.5

" I 1..z 45Omg KCl/L ConductivityIt"j01DO

( mhos/cm)

Temperature 24.1

-1A 1.

pH (S.U.)

-1.b

"..16 q.. 4*L r-,(4-

].qL 1 8*,__

DO7.

0

1.

.5.

1.5 600 mg KCLL Conductivity 13 ooooI

,/LI I

__*mhos/cm)

- IH'13 oI O

Temperature zq2L1 2S.

-PH (s.u.)

-1.

1 "il R

1 1-17ý3 Conductivity ROT)

(Vmhos/cm)

Temperature

(°C) 7q-q.

-1 ZI 2,+9

-ZA. S 11 -t~. R "zZA

".9 2q.s

_pH (S.U.),

I.1 R6 "I'l1.q"L Iq

.A.k.

"]

-I.

-I.B9

" 4,-/.

-DO m,/L)

-.1.-

7

'1.

900 mgKCI/L Conductivity t*go kI3 1(%30lqs j q3 Temperature Z '

~ ~

'lq

"~~.... 1q*

"z.l z..:

_q. (°-

i-q,.1,'2

,,q pH (S.U.)

I.80 715

1.

II m.i

' -ON I

.54 l

q' '7.7

-,'.i Conductivity:

JpmhoskM),

Temperature (0 C)

Iniial

~inai~tial Final Initial l

Init~itia i

-'U

.5 Conductivity (Prnhos/cm),,

.°

.0

I*Environn 1.14.

1.12 1.10 1.08 1.06 1.04 1.02 Ceriodaphnia dubia Chronic Reference ToxicantControl Chart nental Testing Solutions, Inc.

USEPA Control Limits (+2 Standard Deviations)

, *..................i,',

I I

l I

I I

0-*

2.5 2.0 1.5 1.0 USEPA Warning and Control Limits-.(75th and 9 0th Percentile CVs)

I I

I.I 0.5 1.4 1.3 1.2 k I

I I

. t I

I I

Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 1.1 1.0 F 0.9 F 0.8.)

Olt-

"'z

.9 zkyw Z r I e0-,',,' late Z.

7-day IC2 5 = 25% inhibition concentration. An estimation ofthe concentratiohof 'sodium chloride that would cause a 25% redUction i Ceriodaphnia reproduction forethetestpopulation,. *K>,-,

Central, Tendency (mean IC25)..

Warning Limits (mean IC2e +

A.10 or SA.75)

Control Limits (mean IC

+/- S.

S or 2 Standard Deviations)

ýge 8'6 of 98 25 A.25' A.90'

Environmental Testing Solutions, Inc.

Ceriodaphnia dubia Chronic Reference Toxicant Control Chart State and USEPA Laboratory Laboratory USEPA Teat number Teat dat 7-day IC2

.CT S

Control Limits SA1 0 Warning Limits SA.M Control Limits SA.75 Warning Limits S,.,

(g/L NaCp)

(g/L NaC1)

TT-28 CF + 28 C-- - S CT +

CT-SAM CT + SA.I CT-SA.,

C +

s 1

10-0207 1066 2

11 0607 106

, 106 0.00 1.05 1.06 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58

-1.54 0.6 3

11.-007

,1.7 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1,54 0,6 4

-12ý04 2

0 1.07 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.64 5

'01708-08 i09 1.07 0.01 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.61 6

_01j08-08 L 09 i'.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.6 7

,02-05-08

1 07 1.07 001 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59

,-1.56 0.6 8

.03-04-08 1'07 1 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 0.6 9

"04-08-08 6 07 1.07 0.01 1.05 1.10 0.09 0.99 1.16 ti.18 0.89 1.25 0.48 0.59

,1.56 "

0.6 10

.05-06-08' 106 1.07 0.01 1.05 1.09 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59

.1.55 0 0.6i 11

ý'06-3-08

11.

l 107 0.01 1.04 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 1.56 06 12

-i07108-08 107>7-*

.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.26 0.48 059

.1.56 0.6 13 08ý-5-08 1.>-109 1.08 0.01 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59

1.56 0.6 14 09-0908 1*0**

8 m

0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 059

%1.56 0.6 15 10-0108

1.

-,1.08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59

ý1.56 9.06 16 1*1-04-08 10.07 1.08 0.01 1,05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 059 1.56 09.6 17 11-04-08 110 1:08 0.02 1,05 1.11 0.09 0.99 1,17 0.18 0.90 1.26 0.49 0.59 1.56 0.6 18 12-02-08 L106

>'108 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.49 0.59 1.56*

0.6 19 01 f06-09 1.*05 1.08 0.02 1.05 1.11 0.09 0.99 1.16 0.18 0.89.

1.26 0.48 0.59

1.56 06 20 02-03-09 1.05 1.08 0.02 1.04 1.11 0.09 0.99 1.16 0.18 0.89 1.26 0.48 0.59 156 0.6 Note:

7d1,C7,5 7-day 25Yo/inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

CT.= Central;tenidency (mean IC25j).

.1 o0 Con

'.CT - S,.

6 0.40 6.

i0.40

6.

0.41 6,

0.41 7.

0.41 7 -

0.41 7

0.41 61 0.41

7.

041 7:

0.41 7

0.41

7.

0.41 7:.

0.41 7r 0.41 7

0.41 7'

0.41

7 0.41 7'

0.41 JSEPA trol Limits Y CV 0o Cr +'s*

1.72 1.72 1.73 1.73 1.74 1:74 1.74 1.74 174 1.74 1.74 1.74 1.74 1.75 1.75 1.75 1.75 1.74 1:74 0.00 0.01 0.01 0.01 0.01 0.01 0,01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0,01 0.01 0.02 S

Standard deviation'of the IC2; values.

Laboratory Cont roland Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs.

These ranges.are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

IQSASS Stdard deviation corresponding to the 100 percentile CV. (SAio = 0.08)

-,S.

Standard deviation corresponding to the 250 percentile CV. (SAs=0.17)

USEPA Control and Warning Limits

,..Ss =7 Standard deviation corresponding to the 75ta percentile CV. (SA. 5 = 0.45)

S Standard deviation corresponding to the 90' percentile CV. (Sk,, = 0.62) p CeY - Coeffiient of variation of the IC2, values.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

Y

.~

5.b03-09

Precision of Endpoint Measurements Ceriodaphnia dubia Chronic Reference Toxicant Data Environmental Testing Solutions,lncl.

Test Control Control Mean..

nu b r

Test date.....:....'.

number Survjval-,

Reproduction.

(%)

(offspring/female) 1 2

3 4

5 6

7 8

9 10 11 12 13 14 15 16 17 18 19 20 10-02-07 11-06-07 11-06-07 12-04-07 01-08-08 01-08-08 02-05-08 03-04-08 04-08-08 05-06-08 06-03-08 07-08-08 08-05-08 09-09-08 10-07-08 11-04-08 11-04-08 12-02-08 01-06-09 02-03-09 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 29.5 28.0 30.4 31.8 30.3 31.8 31.3 31.5

.29.6 32.1 30.5 30.9 29.4 28.3 32.4 32.3 31.5 31.5 33.2 31.4 CYT for Control Mean Reproduction (offspring/female) 28.8 29.3 29.9 30.0 30.3 30.4 30.6 30.5 30.6 30.6 30.6 30.5 30.4 30.5 30.6 30.7 30.7 30.9 30.9 for Control

(%)

Reproduction CV (%)

(%)

for PMSD (%)

Cv CT MSD'ý PMSD CT 9.4 6.1 5.6 5.5 6.6 4.9 6.4 8.0 7.7 6.3 7.9 6.7 7.9 8.5 7.2 5.1 6.2 4.6 4.0 6.6 7.7 7.0 6.7 6.6 6.3 6.4 6.6 6.7 6.6 6.8 6.8 6.8 7.0 7.0 6.9 6.8 6.7 6.5 6.5 2.4 2.5 2.2 2.5 2.2 2.4 2.8 2.7 2.6 2.5 3.0 2.4 2.2 3.0 2.7 2.2 2.4 2.5 2.0 1.9 8.2 8.8 7.2 7.9 7.3 7.7 9.1 8.5 8.8 7.9 9.9 7.7 7.6 10.5 8.4 6.8 7.7 8.0 6.1 6.0 8.5 8.1 8.0 7.9 7.8 8.0 8.1 8.2 8.1 8.3 8.2 8.2 8.4 8.4 8.3 8.2 8.2 8.1 8.0 Note:

CV.= Coefficient of variation for control reproduction.

Lower CV bound determined by USEPA (I 0h percentile) = 8.9%.

Upper CV bound determined by USEPA (90th percentile) = 42%

MSD = Minimum Significant Difference PMSD = Perce'nt Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statisticallysignificant in a~whole effluent t6xicity test.

Lower PMSD bound determined by. USEPA.(! 0" percentile) = 13%N.

Upper PMSD bou'6ddetermin'ed........

0operentile).= 4,7%9#.

CT =.Central Tendancy (Mean Control Reproduction,: CV, or PMSD)

USEPA. 2000 nderstanding and Accounting for Method V ariability. in Whole Effluent Toxicity Applications Under the National Pollutant Discharge, Elimination Program..EPA:833-R-00-003, US Environmental Protection Agency, Cincinnati, OH.

USEPA. "2001a; 2001 b.: Final Report:. lnterlaboratory Variability'Study-of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-82 I-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

02-03-09 Page 88 of 98

.Ceriodaphnia dubia Chronic Reference Toxicant Control Chart I: EnvronmntlTesing Precision of Endpoint Measurements nvironmen 11Tsýting SolutionsInc.

40 o _ 35, S30 4 2

-25 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)

I I

I III I

I I

I I,

North Carolina Acceptance Limit (< 40.0%)

~ 30

~Kentucky Acceptance Limit (< 30.0%)

o.~20 I

I I

I II I

I I

50 40 10,

.0 Y

a,...

Test date 111fhA a

L.A LLjJA If,

+

rS t

V 4 I

A fl Paw"""*

QLl1 (PMSD)- PMSD.is the minimum significant difference between the control'and treatment that can-be

- i " :-declared statistically'significaht.

Central Tendency (mean Control Reproduction, CV, or PMSD) ge 89 of 98 Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

RN P

Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia S...CdNaCLCR#:

5S age I of 6

'2.

":i, Dilution preparation information:

Comments:

NaCl CHM number:

CIA I2..*55, Stock preparation:

100 g NaCI/1 (dissolve 50 g NaCI in 500 ml deionized water)

Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism source information:

Test information:

Organism age:

< 24-hours old Randomizing template color:

Date and times organisms were born OiL.-i-aO Mao To %*,1 Incubator number and shelf between:

location:

Culture board:

vi\\. -

A Replicate number:

1 3

S 6

7 8

9 110 YWT batch:O Culture board cup number:

as 1

"6 6 5b 131 13 Transfer bowl information:

pH - 1. It SU Temperature =

".'4, 0C Selenastum batch:

10 ______0 Daily renewal information:

Day Date Test initiation and feeding, MHSW Analyst.

renewal and feeding, or batch used termination time I

Ol-oq-o'0 0o$0 016qi-oik &

2 0*- OS-0

0 Po0 Olo oVA

5.

o-z-o o

o 0o 0

4_ _

O" -& 0"!

0p t* l~ OOZ t

05 a

6 o2..0109 olq5 c.r?.~jo.~yj ITe (AI wA't b COntrol information:...

Acceptance criteria Siiummary of test endpoints:.

% of Male Adults:

<20%

7-day LC50

> tI400

% Adults having 3d Broods:

1007.

>80%

NOEC 0O-

%'Mortality:-

5. 20%

LOEC 1- 000 Mean Offspring/Female..:.

15.0 offspring/female.

ChV

.qq.

Lk

%CV:

<40.0 %

IC25 e00I00. S

t.*

o!ge 90 of 98

Page 2 of 6.

Ceriodaphnia dubia

oL, CdNaCLCR#:

Survival and Reproduiction Data Replicate number 1"

.2 3

4 5

6 '

7 8

9 7 Young produced

".C)

C)

Adult mortality I

Youngproduced 0OV" Q

)

C)

.0 1

0 0

o

-J

'~V~.1/4>, ~

'~r-

~,

Adult mortality 1

1 T

r t

T r

T T

Young produced

) 1 0 1 O Of C) 01 C01 C)

In 4

4

+

4 ____

4

~

Adult mortality

%_l \\_.I U__

4 Young,.produced 1

I Li U

1 A

L Adult mortality I-5 Young produced i~

i'

' i LX

__ -L i

'A Adult mortality 6 Young producedI ILA 1

< -I I J 1 16 1 3

Adult mortality Young produced Total young produced 2-S Final Adult Mortality X for 3rd Broods 1>4

Note-Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

% Mortality:

0 C).

Mean Offsprin emale:

31-1A 600 mg NaCI/L Survival and Reproduction Data Replicate number Day 1

2 3

4 5

16 7

8 9

10 I

Young produced

(:ý)

cC 0 0*

1D C) 01 0*O

(

Adult mortality L.

JL LI t_.

2. Yung produced__

p QJCp p

YoAdult mortality I

fLoungjproduc]d2 I-%

3 Youngproduced HI C t0-0 C) 0

% ITI H.

f o

Adult mortality L-L_

L 4 Yung produced t4i

(-.

6L

]~

~ L1 Adult mortality L

U j"

Lj 5[

Young produced 1.~F 1~ 10 i~

~I~

~I'~

7

[, Youngpr*oduced

-S I LA LS Adl

-otlt

%I. -.

Total*young produced 01

.30 a

z 0

L

.3 cge 91 of 98 Final Adult Mortality Note: Adult mortality (L = live, D = dead), SB= split brood (single brood split between two days), CO = carry-over (offspringS 0.

cairie'dover' with adul during transfer).*

I Loncentratton: "

1~7~i~

% Morta'lity:

Mean Offspring/Female:

32-. 0

% Reduction from Control:

- w-It '7

a Page 3 of 6 Ceriodaphnia dubia CdNaCLCR #:

rData Survival and.

Concentration:

% Mortality:

07.

Mean OffsOring/Female: -

tAi, I

I I

1

% Reduction from Control: I O. n.

1 1000 miz NaCI/L Survival and Reproduction Data Replicate number Day 1

2 3

4 5

6 7

8 9

10 I

Young produced 0(*

_ Adult mortality [

JL.1__

1[_..

L t

,.t L_

2 Young produced O

[

3 Young produced 0NT C) 07[

Adul t mortality

\\_

Q L

IL 4 Young produced LILL 4 I

Adult mortality L_ I._-

L-1'--

%--1

-]

I-_

5 Youngproduced 1

10 1C.

t I

l-12

.L "1-Adult mortality [UV.

T

.. V

_uI-

I'--

6 Young produced IA I

0L 12L...

Adult mortality-U 7

Youngproduced]

Total young produced

+,

z" 2,

74 O

Z2 Final Adult lvMortality L

TJ L..

1-I l?....

t J.I.

t~t'.

n l*

I t,

fl*

1.-i

--- l-*-

rage 92 of

'7

:..+

P.ore:,dult mortaity kL= live, D,= dead), SB = split orooa (single orood spit

.etween two aays), CO= carry over tou pInn carried over with adult during transfer).

98 Loncentration:

% Mortality:

1 26 j 2<

Mean Offspring/Female:

128.1

% Reduction from Control:

I, CM

RAM Page 4 of 6

.CdNaCLCR#:

Survival and Reproduction Data Sipecies: Ceriodaphnia dubia

-1200 ml NaCIL ;,

,Replicate number' Day 2

3

-47f 5

6 7

8 9 1-10".

_!..1*-

V Youngpjroduced

-Q ___-___

C____*__'_O V [

Idunlpr°f"ced0-1 0' o 1

Adult mortality L-L..

L..

4 Young produced 0

L 0

C[I*

H j

Adult mortality 6

JYoung produed CT Adult mortality j~~f\\1

- 'i~

5 Young produced

t.

C)

Total young produced

_-_ iI a

I '( T 01 1,

Final Adult Mortality

\\-

l Note: Adult mortality (L = live, D = dead), SB split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

% Mortality.

Mean Offspring/Female:

% Reduction from Control:

15-.127.

1400 mg NaCI/L Survival and Reproduction Data

--I

_ _ __ _ _Replicate number 7

1 8

1_9_10 Day 1

2 3

4 6

758 9

10

1)

I-1111 0

1 Young produced 0

Adult mortality t-O LO Z7,_

\\. O

\\-..

Adult mortality F...1 I.

1 T

SE LI

%.I+KL YoungproducedI0 0

C) 0_

0I 1, 0l YoAdunt mota lit

_-- I I % I I

LlI 4

Young produced

-LI _

Adult mortality 5

Young produced 0

" I Adult mortality Iz l

6.

Y*ung.produced. IQI 0-i,0-*

I L

'L

_1 U

0 F

4

.:fAdult mortality V_____

I--

I'-~.-[I-I-

7-r'

'7-Youngprdued i

'T6t6lyoung oroduced a

2..

2.

.3 3

I 2.-,

Final Adult Mortality'

'C.

C.

AI-L~

AAun

£I~van u.v' s-udj TD jlbuuu

~igcUIO

=jJI; rWVI

=AO

'Al5J On.A I

L 4

r JYJ

-Vi

.0 aI ii"

'rage 93 of 98 carried over with adult during transfer). "

-.Concentration:

% Mortality:

01.

Mean Offspring/Female: 5

% Reduction from Control:

'i1.. "

j* Enironnnntal T Stlgsolutions. Inc.

Contro1li-?:![,i, r'

Verification of Ceriodaphnia Reproduction Totals 1000 mg NaCIIL Day Replicate number rTotal 2:.

2 3,',

4 5

6 7

8 9

10 1

0:

0' 0

0 0

0 2

0 0,-0 0

0 0

0 3

.0 01,

-0" 0

0 0

0 4

1 6 4!

.5',

5 4

6 4

4 5

5 48 5,

111 13 12ý 12 12 14 12 14 13 12 125 6

14 14 16 13 12 15 14 16 13 14 141 Total 31 31 33.*

30 28 35 30 34 31 31 314 600 mg NaCIJLU" Re licate number Day 1

1-3 4

5 6

7 8

9 10 Total 1

0 0

0 2

0 0

0 3'

H 1 0 0'

0 0

4 5

4.

.6' 6

5 5

4 4

-5

5 49 5:

14 12 12 13 11 14 14 13 12 10 125,

14 15 14,-

16 13 15 15 15 14 15 146

,7, mllmmmmmmm Total 33

31.

,32-35 29 34 33 32 31 30 320.

800 mgNaCIl/L Da 2:

3,,,*.

Replicate number Total Day 1

2' 3

4 5

6 7

8 9

10 1,

0 0

-.0,

0 0

'2' 0.,

0 0

3 0

0 1.0 0

0 0

0 4'

5 -

4

-,5' 5

5 4

4 4

6 5

47 5

12 10 '.12 15 12 14 14 12 12 12 125 6

13 14 16 14 14 14 14 15 12 16 142 Total 30

,28 33 34 31 32 32 31 30 33 314 Replicate ounei Day 1

2 3

1 4 5

6'

7.

8 9

10 Total 1

0 0

.0

".0 0

0 0

2 0

0 0

-- 0 0

0 0

3 0

0 0

0

-0

't.0 0

0 0

4 5

5 4

6 4

4 5

ý5 5

5 48 5

11 13 10 10 13 12 111. 1:41 12 12 12 116 6'

14 13 11 13 12 10 15ý-

.12 12 11 123 Total 30 31

25.

29 29 26 31 F 29 29 28 287 1200 mg NaCIIL Replicate number Day 2

3 4

5 6

7 1:'8 9

10 1

0 0

.0 0

0 0

.0 0

0 0

0 2

0 0

0 A0 0

0 0

3 0

0 0

,0 0

0 0

4 3

5 4

3.

4,,

4 4

4 38 5

0 3

5 3

6 6

0 t5 5

2 35 6

0 0

6

.0 0

5 11 Total 3

7 8

8 10 9

10 1 9 9

11 84 1400 mg NaCIIL Replicate number

" Total Day 1

2 3

4 5

6 7

.8 9 110 1

0 0

0 2

0 0

0

.0 0-0 3

0 0

-0Q 0o.

0-0

0 0

4.

0

.2 2

1 2

0' -,3.50 '0 2

1 5

3 0

0 2

0 13 ;'0 "4

1 3:

6 0

0 0

00 0

130 I

'1

":00 Total

.3 2

2 3

3 1

.4 r

Ii Ceriodaphnia dubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.

Test number-CdN&(3CR #85 Tesfdatc;s:"'ý February 03-09, 2009.

Reveiwed by:

Concentration

-_..._....Replicate number Survival Average reproduction -

Coefficient of Percent reduction fromn (mgfL NaCI) 1 2

3 4

.5 6

7 8

9 10

1)

(offspring/femaale) vai tio(

control(

Control 31 -

31--

33 30 28 35 30 34 31 31" 100 31.4 6.6 Not applicable 600 33 31 32 35 29 34

.33 32 31 30 100 32.0.

5.7

-1.9 800 30

.28 33 34

'31 32 32 31 30 33 100 31.4.

5.7 "

0.0 1000 30

- F3jl-25 29 29 26 31 29 29 28 100 28.7'

6.8.

8.6 1200 3

7 8

8 10 9

10

9.

9 11 100 8.4

,26A4 73.2 1400 3

2 2

3 2

3 3

.4 1

2 100 2.5 34.0 92.0 Dunnett's MSD value:

1.876 4 MSD _

Minimum Significant Difference PMSD:

6.0 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and teatmenttthat'can*be declared statistically

",.c "

significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) 13%.

Upper PMSD bound determined by USEPA (90 percentile) 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMISDdaa from EPA's WET Interlaboratory t'

Variability Study (USEPA, 2001a; USEPA, 2001b

.b...

-s USEPA. 2001a, 200 lb Final Report Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix.:EPA-82 l-B 004 'and EPA-82,B-01005.

US Environmental Protection Agency, Cincinnati, OH.

f

0 Statistical Analyses Enviromental qesung Solutlons, Inc.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:<Tiýý2/3/2009.

Test ID:

CdNaCICR Sample ED:

REF-Ref Toxicant End Date: "2I9/2009' Lab ID:

ETS-Envir. Testing Sol.

Sample Type:

NACL-Sodium chloride Sample-Date:.

Protocol:

FWCHR-EPA-821-R-02-013 Test Species:

CD-Ceriodaphnia dubi"a Comments:

Conc-mg/L

1.

2 3

4 5

6 7

8 9

10 D-Control:-; ; :731:000 31.000 33.000 30.000 28.000 35.000 30.000 34.000 31.000 31.000: -

600:

33.000 31.000 32.000 35.000 29.000 34.000 33.000 32.000 31.000 30.0006 800--

30.000 28.000 33.000 34.000 31.000 32.000 32.000 31.000 30.000 33.000" 1000 30.000 31.000 25.000 29.000 29.000

'26.000 31.000 29.000 29.000 28.000 1200 3.000 7.000 8.000 8.000 10.000 9.000 10.000 9.000 9.000 11.000 1400.

3.000 2.000 2.000 3.000 2.000 31000 3.000 4.000

.1.000 2.0001 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L -

Mean N-Mean Mean Min Max CV%

N t-Stat Critical MSD Mean

-N-Mean D-Control:

31.400 1.0000 31.400 28.000 35.000 6.578 10 31.7006, 1.0000 600 32.000 1.0191 32.000 29.000 35.000 5.705 10

-0.731 2.287 1.876 31.700 1.0000 800 31.400 1.0000 31.400 28.000 34.000 5.657 10 0.000 2.287 1.876 31.400 0.9905

1000'

'28.700 0.9140 28.700 25.000 31.000 6.782 10 3.290 2.287 1.876'

.28.700

-', 0.904,

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1400

2.500 0.0796 2.500 1.000 4.000 33.993 10 35.219 2.287 1.876 2.500 0.0789 Auxiliary Tests.,

Statistic Critical Skew

, Kurt-,

Kolmogorov D Test indicates normal distribution (p > 0.01) 0.95103425 1.035

-0.5620922 0.76.156552.-,

Bartlett's Test indicates equal variances (p 0.18) 7.56523657 15.0862722 Hypothesis Test.(1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE.

F Prob df.

Dunnett's Test 800 1000 894.427191 1.87636754 0.05975693 1771.72 3.36666667 1.7E-44 5 54 Treatments vs.D-Control Linear Interpolation (200 Resamples)

Point

<mng/L SD 95%CL Skew IC05 895.185185 39.6216408 798.428309 967.186364

-0.4820 ICIO.

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-1.3664 IC15 1017.29064 6.93088301 1003.19782 1027.02713

-1.7330 IC20 1032.9064 5.81256688 1019.78841 1041.72271

-0,5668 IC25 1048.52217 5.49826611 1036.37899 1057.13228

-0.5823 IC40 1095.36946 4.95417378 1084.62237 1102,73588

-0.5583 IC50*

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-0.4842 02-03-09

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Species: Ceriodaphnia dubia Page 6 of 6

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PERMITTEE NAtMEIADDRESS

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my L

1TELEPHONE

DATE, direction or supervision in accordance with a system designed to assure that qualified personnel j

I Timothy P. Cleary-properly gather and evaluate the information submitted Based on my inquiry of the person or I

persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer

-i:

41 Sequoyah sitteVice: restdenpt information, the information submitted is.to the best of my knowledge and belief, true, Enin eer

-`42 8360009 0

"10 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE DAY i nformation, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT

AREA, NUMBER YEAR

,TYPED OR.PRINTED

_....__FFCERORAUTHOR__EDAGNT__,,_CODEI____"__"_"_"c.'M COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPAFom 32-1 RE 399 Prviu edtin ma beue-aeo EPA Form 3320-11 (REV 3199)

Previous editions may be used Page I of 1

PERMITTEE NAME/ADDRESS" (Include Facilitv Name/Location if Different)

Name TVA-- SEQUOYAH NUCLEAR PLANT Address PO. BOX 2000.

INTEROFFICE SB-2A)

SODDY - DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY, r ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

"MB No. 2040-0004 TN0026450 107 G F -FINAL PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND MONITORING PERIOD EFFLUENT I YEAR MO DAY I YEARI MO I DAY From 09 02 01 To 097 02 28 NO DISCHARGE NOTE: Read instructions before completinq this form CENTRATION NO

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NAME/TITLE PRINCIPAL-EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

, ty Ce i.:

direction or supervision in accordance with a system designed to assure that qualified personnel

',-TImnothy P. Cle'ary K

properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Sequoyh Site Vtce President accurate, and complete. l am aware that there are significant penalties for submitting false Y

T

/ information. including the possibility of fine and imprisonment for knowing violations.

TYPED OR PRINTED' Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT

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-423 843-6700 09 03 1 -10 AREA NUMBER.

YEARý MO DAY CODEI COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No DischargethisPdri*d d.

EPA Form 3320-i'(REV 3/99)

Previous editions may be used EPA orm332-1 REV319)

Pevius ditonsmaybe sedPagel1of I

PERMITTEE NAME/ADDRESS.(Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

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SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY-ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES).

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(SUBR 01)

TNO026450 110 G F - FINAL PERMIT NUMBER DISCHARGE IN-IMiER RECYCLED COOLING WATER Form Approved.

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NAME/TITLE. PRINCIPAL EXECUTIVE OFFICER I Cerfify under penally of law that this document and all attachments were prepared under my j'

j

~TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel

.Timothy P. Cleary' properly gather and evaluate the information submitted. Based on my inquiry of.the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Principal"EnvironmentalEngineer 423-843-6700 09 03 10 accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including-the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER M

TYPED OR PRINTED

_._OFFICERORAUTHORIZEDAGEN_

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E AR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Peri6d EP or

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.rv. u ed.on.abeued.Pge.

EPA Form 3320-1 (REV 3/99)

Pre vious editions maybe used Page 1 of 1

PERMITTEE NAME/ADDRESS (IncludeFacility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

_LINýTEROFFICE SB-2A)

SODDY - DAISY

TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01).

TN026450 J

110 T F - FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER

! MONITORING PERIOD EFFLUENT Form Approved.

OMB No. 2040-0004 YEAR M

DAY YEAR MO DAY S

From1 09 I02L01 1 ToF 09 02 128 NODISCHARGE XX NOTE: Read instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

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______________i NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my i

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}

persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 4

8 Sequoyah Site Vice President information, the information submitted is, to the best of my knowledge and belief, true,423 843-6700 09 03 10 accurate. and complete, I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE T"___

information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3/99)

' Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA. SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A)

SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

TN0026450 7

116 G77 F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH Form Approved.

OMB No. 2040-0004 htAWITflRINC PRIi')nf I EFFLUENT YEAR MO DAY YEARI MO I DAY NODISCHARGE From 1 09 02 01 I To I 12 8

NOTE: Read instructions before comoletinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION I

NO.

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vf NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my p

1 TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary -

properly gather and evaluate the information submitted. Based on my inquiry of the person or I

persons who manage the system; or those persons directly responsible for gathering the Principal 03 information, the information submitted is, to the best of my knowledge and belief, true, Environmental Engineer 423 843-6700 09 1 03 10 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE PRINTED " :information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY 1 TYPED OR PRINTED OFIE'RATOIE G

TYA MOD 1-i A"

[

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Operations performs visuial inspections'for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

(.ITEROFFICE SB-2A)

SODDY - DAISY-TN 37384 Facility TVA-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) " MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR Q*,

TN0026450 117 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH L

MONITORING PERIOD EFFLUENT Form Approved.

OMBNo. 2040-0004 FoI YEAR I MO I DAY IYA From 1 09 1 02 -F-01 To 1 09 02 28,

NO DISCHARGE Z NOTE: Read instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my A

TELEPHONE DATE

,direction or supervision in accordance with a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or O

persons who manage the system, or those persons directly responsible for gathering the

-Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 0.3 10 Sequoyah Stte Vice Prestdent accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

i nformation, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEARI MO I DAY TYPED OR PRINTED ICODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference.all attachments here)

Operations performs visual inspectiors for floating debris and oil and grease during all backwashes.

EP.om 3 2

-1 (RE 3/9.rvtu.dtin.ab s dPa e

1o EPA Form 3320-1 (REV 3/99)

,: Previous editions may be used Page 1 of 1

P ERMITTEE.NAME/ADDRESS (Include Facility Name/Location if Different Name TVA-SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

_LINTEROFFICE SB-2A_)

SODDY - DAISY TN 37384 Facility TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie'A. Howard F

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

OMB No. 2040-0 TN6 265 0 K118

-G7 F - FINAL PERMIT NUMBER

[DýISCHARGE NUMBERI WASTEWATER & STORM WATER MONITORING PERIOD EFFLUENT DAY R

YEA I

MD I D

- A NO DISCHARGE X X NOTE: Read instructions before completinl this form.

004 PARAMETER 1

QUANTITY OR LOADING QUALITY OR CONCENTRATION No.

FREQUENCY1 SAMPLE IEX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)

SAMPLE 19 MEASUREMENT 19 00300 1

0 0

o

.PRMIT.

2,0 Ill WEE

,GRAB EFFLUENT GROSS VALUE REQUIRAMENT

.ILY MN SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 19 00530 1

0 0

10I0.

MGIL 7'7 TWICEI.GRAB 1 EFFLUENT GROSS VALUE

-EQUiREME*NT

'<J

.2?7*:

Qi:

-* DAILY MX WEEKi<

SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 25 00545 1 :.0 0

PERMIT 1.0 MLLOC/

RAB

!:REQUIREMEN:T?

!

: :,:CNC i

GP B.

EFFLUENT GROSS VALUE REURMN AL XMONtH FLOW, IN CONDUIT OR THRU SAMPLE 03**

TREATMENT PLANT MEASUREMENT 03 50050 1

0 0

~

EOT EOT MGD

- - - 4,

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EST IIMIA I, RE U1E 1N SAMPLE EFUU MEASUREMENT

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1 SAMPLE MEASUREMENT iPERMIT I

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4444'

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SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my l..

ii TELEPHONE 1

DATE direction or supervision in accordance with*a system designed to assure that qualified personnel Timothy P. Cleary properly gather and evaluate the information submitted. Based on my inquiry of the person or 44 0'*

OL

'40(

')C persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 09 03 10 Sequoyah Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED

.CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1..(REV 3/99)

Previous editions may be used Page 1 of I

ML090760607

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Dear Mr. Patrick Cromer:

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ML090760607

Text

Dear Mr. Patrick Cromer:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

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