Text

Discharge Monitoring Report for June 2007
	ML071990319
Person / Time
Site:	Sequoyah
Issue date:	07/13/2007
From:	Howard S; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML071990319 (109)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 July 13, 2007 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 I Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JUNE 2007 Enclosed is the June 2007 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sicerely,~ Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Printed on recycled paper

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 .__ INltTE.OFFICE S B-2A.) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01 ) PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved. OMB No. 2040-0004 I M)NITCRING P:RICi' I EFFLUENT YEAR MO DAY YEAR MOI DAY From L 07 1 06 I011 To 1 07 06 30 ATTN: Stephanie A. Howard NO DISCHARGE F-] NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE

- - 29.7 04 0

30 / 30 MODELD CENTIGRADE MEASUREMENT 00,010 Z 0 0 PERMIT 30.5 DEGO. CSEE CK:REQ INSTREAM MONITORING REQUIREM:DENT PERMIT TEMPERATURE, WATER DEG. SAMPLE 41.6 04 0 30/ 30 RCORDR CENTIGRADE MEASUREMENT**0 00010 1 0 0 -- PERMIT REPORT~~**~~ )***i DE.C. SEE. CK REQ REQUIREMENT ,~ ,;EOT PRiT EFFLUENT GROSS VALUE i<f~&. I>~ PERMIT~-~ ALYMU TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2.6 04 0 30 / 30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 -~~PERMIT> 3.0~*>K DEG. C. CONTIN CALCTO EFFLUENT GROSS VALUE REQUIREMENT .O"S i: UM~DILYIIA MXOP PH SAMPLE 7.1 7.5 12 0 4/30 GRAB MEASUREMENT EFFLUENT GROSS VALUE REUREETU NU-M.MAIU SOLIDS, TOTAL SUSPENDED SAMPLE 10 10 19 0 1 / 30 GRAB MEASUREMENT 00530 1 0 0 P.ERMIT 3* 100.

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MGIL MONTHL*_Y GRAB EFFLUENT GROSS VALUE REQIREEN MOIAVG >DA -ILY Mx OIL AND GREASE SAMPLE

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~ CONTIN RCORkDR~ EFFLUENT GROSS VALUE 'EtRET <DAILY MXU.21 UOU& NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under m .( j fTELEPHONE DATE -direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or ,L 1 persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 42 83-70 7 07 1 SiteVicePresdentinformation, the information submitted is, to the best of my knowledge and belief, true, 423____843-6700_______07__07___13_ SiteVicePresdentaccurate, and complete. I am aware that there are significant penalties tor submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPED_______OR____PRINTED information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No closed mode operation. The following information is included in an attachment: 1. thermal compliance information 2. CCW data 3. veliger monitoring data EPA Fom 33.-...RV.3.9).Preious eition may:e use Page1 of* EPA Form 3320-11 (REV 3/99) Previous editions may be used Page 1 of 2

NPDES Permit No. TN0026450 Sequoyah Nuclear Plant Notification of Adjustment to Numerical Model This notification is provided in accordance to Part III.G of the NPDES permit, which requires TVA to inform the Division when adjustments are made to the numerical model used to determine compliance with the requirements for water temperature for Outfall 101. The model was removed from service at the beginning of the reporting period for May 2006, and was returned to service at the beginning of the reporting period for June 2007. Provided herein is a brief description of the model, the problem that forced the model out of service, and the adjustments that were made to return the model to service. In the preferred method of compliance for Outfall 101, the water temperature at the downstream end of the assigned mixing zone is determined every 15 minutes by a mathematical model based on: (1) the measured flow, temperature, and depth of ambient water in the river; and (2) the measured flow and temperature of water entering the river from SQN. Measurements for the model are made by ambient river temperature and stage monitors located at the intake skimmer wall (Station 13), by flow and temperature monitors located at the plant discharge diffusers (Station 12), and by flow monitors located at Watts Bar Dam and Chickamauga Dam. The use of a mathematical model is necessary because it is not possible to locate instream water temperature stations in the river navigation channel, where mixing of the plant thermal effluent occurs. A backup monitor (Station 8) is provided near the shore of the. river at the downstream end of the mixing zone, but this location is not situated in the middle of the diffuser plume and can misrepresent the impact of the plant effluent on the river for certain flow conditions. In May 2006, TVA discovered that the numerical model was consistently underestimating the river temperature compared to measurements at the backup monitor. The discovery was related to changes in the behavior of the diffuser effluent resulting from a sustained period of low flow in the Tennessee River earlier in March and April. In this event, heat from the diffusers was migrating upstream in the river further than that anticipated from previous studies. At that time, the flow in the river was the lowest since 1981, and lower than other historically extreme low flow years, such as 1988. The temperature differences were not observed in the earlier years because in 1981 SQN was operating with only one unit in service, and in the first part of 1988 there were no units in service. TVA has learned that conditions creating such events have occurred even in years with higher river flow. However, based on previous understanding of the behavior of the plant thermal effluent, such events never attracted attention. Recent studies have now led to an improved understanding of the mixing and transport of the plant diffuser effluent at low river flow. There are two fundamental reasons why the model was removed from service in May 2006. First, to ensure that the ambient temperature measurement is not biased by heat migrating upstream from the plant, a new ambient temperature monitor, Station 14, was installed in the river about 5.7 miles upstream of Station 13. The numerical model did not account for an ambient temperature measurement at Station 14. Second, the model did not adequately account for the local buildup of heat that occurs in the river at sustained low river flow. The model assumed that the heat from the SQN diffusers was all transported downstream when, at sustained low river flow, a portion of the thermal effluent lingers in the river and migrates upstream. I

To correct for these factors, the numerical model was adjusted to incorporate the proper ambient temperature and to estimate the local buildup of heat in the river that occurs at sustained low river flow. The former was accomplished simply by importing and assigning the ambient temperature from Station 14 rather than from Station 13. To estimate the local buildup of heat, the model formulation was expanded to include the impact of sustained low river flow as reflected by a dimensionless parameter known as the densimetric Froude number. In the SQN situation, the densimetric Froude number represents the ratio of the river momentum force to the thermal plume buoyancy force. The river momentum force is a measure of the ability of the flow to flush the thermal effluent downstream, whereas the plume buoyancy force is a measure of the ability of the thermal effluent to linger and move upstream in the surface layer of the river. When the river momentum is large compared to plume buoyancy, the thermal effluent tends to be transported downstream, and when the river momentum is small compared to plume buoyancy, a portion of the thermal effluent tends to migrate upstream and become re-entrained into the thermal plume. Mathematically, at a given point '"p" in the upstream temperature profile input into the model, the densimetric Froude number is computed by Vr j9 PP )(ZP-where Vr is the river velocity, Zp-Zb is the elevation ofp above the bottom of the river, pp is the water density at p, po is the density of the effluent plume at the 5-foot compliance depth, and g is the acceleration of gravity. If Fr is greater than 1.0 (momentum greater than buoyancy), the model assumes the thermal effluent is transported downstream, and if Fr is less than 1.0 (buoyancy greater than momentum), the model assumes that part of the plume migrates upstream and is re-entrained into the thermal effluent, thereby mimicking the local buildup of heat that occurs at low river flow. An example of the performance of the original vs. adjusted model is given in Figure 1, which compares computed 24-hour average downstream temperatures with those measured at Station 8. The comparison is shown for the period in late March and early April, 2006, when the impacts of sustained low river flow first began to emerge. As noted, during this period, the original model yielded temperatures consistently below the actual measurements, whereas the temperatures given by the adjusted model follow the actual measurement much more closely. Additional details about the model adjustments will be given in a calibration report provided with the next NPDES permit application. The calibration study is required once per permit cycle, as outlined in Part III.G of the permit. The last study was issued by TVA in June 2003 for the current permit cycle. 2

70 + LL 6 5 ti "i N. N E. E S 4 50 Kii"" A -.X 3. 45 4'. ~ ~ ~ ~ ~ M -M\\,"~"' ~ 8 4 4...........R, ~~~~~~io~~~~~~~-: 4 ý9".4"9%I~'*' 4 5.........,... 15t ~ t%,--,-%%.k,%*4* ,ýýkt* ý,.,,,*-..*ýý 44* R*Mn T' .N C4 A 200 3/25 3/26 3/27 3/28 3/29 3/30 3/31 4/1 4/2 4/3 4/4 4/5 4/6 417 4/8 4/9 4/10 2006 F igure 1. 24-Hr Avg Downstream Temperature with Original and Modified Numerical Models. 3

June 2007 DMR Attachment June 2007 CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/06/2007 @ 0845 0.22 mg/L 06/09/2007 @ 0817 TSF EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 06/06/2007 @ 0840 <0.10 mg/L 06/09/2007 @ 0849 TSF EPA 8015 May/June 2007 Velicier Monitoring Information NOTES: Mean Water Mean#of Water COLLECTED Sample Date

of Settlers Temp.

Sample Date Asiatic Temp. LOCATION LOCATION Gravid BY ZM/m 3 (C) Clams/m 3 ('C) Asiatic Clam 05/02/2007 05/11/2007 05/17/2007 0 0 0 0 0 0 17 17 17 05/02/2007 05/11/2007 05/11/2007 71 17 86 17 202 17 Inplant Inplant Inplant RCW RCW RCW Dick Adcock Dick Adcock Dick Adcock No veliger samples were taken in the month of June.

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O.BOX 2000 .ffNTEROFFICLE SB-2A SODDY - DAISY TN 37384 Facility_ /A - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TNO026450II 177 1LTG F - FINAL PERMIT NUMBER DISCHRGE NUMBER DIFFUSER DISCHARGE MONITORIN PERI D EFFLUENT Form Approved. OMB No. 2040-0004 YEAR MO DAY YEAR MO DAY From 07 6 011 T o 07 0 30NOTE:ReatNO D ISCHA RG E NOTE: Read instructions before comoletine this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL SAMPLE 0.019 0.041 19 0 26 / 30 GRAB MEASUREMENT 50060 1 0 0 PERMIT. f ~.~~MIL~ EK CLCTD EFFLUENT G~~ROSS VALUE -",T.. .*-...REQUIREMENT. EFFUEN GRS VALUE MO AVG~ INST MAXi EjA~YS"~.&-~ TEMPERATURE - C, RATE OF SAMPLE 30 / 30 CALCTD CHANGE MEASUREMENT 82234 1 0 0 K.PERMIT DE ~REQUIREMENT, 2 DGCONTIN ~CALCTD EFFLUENT GROSS VALUE I I DAL MX C/HR I ,U.USv. SAMPLE ALA MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT ~PERMIT 1 REQUIREMEýANT ,.>P SAMPLE MEASUREMENT .REQUIREMENT.......... SAMPLE MEASUREMENT ~REQUJIREMENT .;~,< SAMPLE MEASUREMENT -~P-ERMIT. ~~ ~ ~<Z NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 114 C ,L d TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel / LJ'l J. Randy Douet properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system. or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 07 13 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE CUMMEN 1TS ANU EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The following injections occured: 1. PCL-222 Copolymer (max. calc. conc. was 0.01r8mg/L--limit 0.2mg/L) 2. PCL-222 Phosphate (max. calc. conc. was 0.052mg/L--limit 0.2mg/L) 3. Biodetergent 73551 (max. calc. conc. was 0.018mg/L--limit 2.Omg/L) 4. MSW-101 (max. calc. conc. was 0.09mg/L--limit 0.2mg/L) EiSA t'orm 33.~U-1 IR~V 3I~.B) PrevioUs erlif ions may be used Page 2 of 2 E:PA Form 3320-1 (REV 3/99) Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _LIN~TEROFFICE SB-2A). SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01 ) TN0026450 101 Q 7 F - FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved. OMB No. 2040-0004 I I I El-I-LUI:N I MONIT(C)RIN(r PFRIOfl I EFF-LUENT iYEAR Mo "iDAY "*... 'YEAR*f MO DAY From DA4Yo1 To 0 06 DA0Y NO DISCHARGE NOTE: Read instructions before completinq this form. ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS BORON, TOTAL SAMPLE

.<0.20 19 0

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&G / O*TR>d> LY.. .RAAB> EFFLUENT GROSS VALUE REQUIREMENT %f~ SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT--------- APELMT REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT REQUIR0E'MENT~ ,~~~,. SAMPLE MEASUREMENT <PERMIT, ~~.h, 5.,,5 SAMPLE MEASUREMENT <-PERMIT 5 .<5~~~.,5. 73/4 j, REQUIREtMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ilTELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel 1.JIt3 .c J. Randy Douet property gather and evaluate the information submitted. Based on my inquiry of the person or /4 .j"L' persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 42 83-70 7 07 1 SiteVicePresdentinformation, the information submitted is,to the best of my knowledge and belief, true, 423____843-6700_______07__07___13_ Sit VcePreidntaccurate, and complete. l am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED______ OR____PRINTED information, including the possibility offine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYE RIRNE CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Boron was sampled on 04/04/2007 @ 1045. EPAQ Form 3320-"1 (REV; 3199) Previous editions may be used Page 1 of 1:5':*d**. ,.:,,* :t ?j*,,** *, 7, ¢, !j-**.'*

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PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ....INTEROFFIE SB-2A) SODDY - DAISY TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) PERMIT NUMBER I DISCHARGE NUMBER MONITORING PERIOD YEAR MO DAY I YEAR M DAY From 07 06 01 To 07 06 30 MAJOR Form Approved. (SUBR 01) OMB No. 2040-0004 F - FINAL BIOMONITORING FOR OUTFALL 101 EFFLUENT NO DISCHARGE NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE __________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >1 00.0 23 0 1 / 180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERiT1 45.2

PERCENT SEE COMPOS*

REQUIREMENTMIMU EFFLUENT GROSS VALUE MI-NIU PER!1IT IC25 STATRE 7DAY CHR SAMPLE />100.0

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~~~A A' AA ~REQUIlREMENT $~' V' /4'A~___ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet property gather and evaluate the information submitted. Based on my inquiry of the person or "L persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 07 07 13 information, the information submitted is. to the best of my knowledge and belief, true. 4 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations, OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Toxicity was sampled 5/28/07 - 6/2/07. Report is attached. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

July 5, 2007 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, MAY, 2007 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected May 28-June 2, 2007, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Fathead minnows and daphnids were not significantly different from control. In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using U`V exposure for pathogen removal prior to introduction of test organisms. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment): Sherrard, R. M.- PSC IX-C Files, RSO&E-EDMS-Muscle Shoals SQN May 2007M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: -July 5, 2007

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3.

NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5.

Design Flow (MGD): 1579

6. Receiving Stream: Tennessee River (TRM 483.6)

7.

1Q10: 3491

8.

Outfall Tested: 101

9. Dates Sampled: May 28-June 2, 2007

10.

Average Flow for the 24-h Sampling Periods (MGD): 1574, 1579, 1578

11.

Pertinent Site Conditions: H-150M non-oxidizing biocide was injected from May 29 - June 1, 2007. The dates and times for the H-150M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period. Date/Start Time Date/Ending Time Injection Location(E)ET (ET) (ET) 05/29/2007 @ 0950 05/31/2007 @ 1035 05/31/2007 @ 1155 06/01/2007 @ 1152

12. Test Dates: May 30-June 6, 2007

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Datphnids (Ceriodaphnia dubia) Page 1 of 92

15. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UVtreated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC9 5 = 45.2%

17. Test Results: Outfall 101: Pimephalespromelas: IC2 > 100%

Ceriodaphnia dubia. IC25 > 100% UV treated Outfall 101: Pimephales promelas. IC2 5 > 100%

18. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected May 28-June 2, 2007, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. 2 Page 2 of 92

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (°C (mg/L) Last Use 101 05/28/07 0800 to 05/29/07 1717 3.8/1.6' <0.10 05/30/07 1302 05/29/07 0700 05/31/07 1250 05/28/07 0822 to 05/30/07 1302 Intake 05/29/07 0722 05/29/07 1717 1.0 <0.10 05/31/07 12 05/29/07 0722 05/31/07 1250 101 05/30/07 0751 to 05/31/07 1412 2.7/3. 6t <0.10 06/01/07 1247 05/31/07 0651 06/02/07 1205 05/30/07 0811 to 06/01/07 1247 Intake 0/10071 05/31/07 1412 2.2 <0.10 062/715 05/31/07 0711 106/02/07 1205 06/03/07 1207 101 06/01/07 0758 to 06/02/07 1339 5.6 /5.8t <0.10 06/04/07 1206 06/02/07 0658 06/05/07 1300 06/03/07 1207 06/01/07 0814 to063/717 Intake 06/01/07 0814 06/02/07 1339 2.2 <0.10 06/04/07 1206 06/02/07 0714 06/05/07 1300

TRC = Total Residual Chlorine

'Samples were collected in two 2.5 gallon cubitainers. Temperature and TRC were measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0LC) in a warm water bath.

Aliauots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. 3 Page 3 of 92

Pimephales promelas Test Organisms: Ceriodaphnia dubia In-house Cultures <24-hours old

1. Source:

2. Age:

Aquatox, Inc. 21 hours old Test Method Summary:

1. Test Conditions:

2. Test Duration:

Static, Renewal Static, Renewal 7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3.

Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5.

Organisms per Replicate: 10

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101

8. Test Temperature: Outfall 101:

10 1 05/30/07 1302 ET 05/30/07 1243 ET 06/06/07 1309 ET 06/06/07 1252 ET Mean = 24.6°C (24.1 - 25.1 OC) 05/30/07 1100 ET 06/06/07 1140 ET Mean = 24.8°C (24.5-25. 1°C) Test Temperature: UV-Treated Outfall 101: Mean = 24.6°C (24.1 - 25.1 C)

9. Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinnevville, CA). 4 Page 4 of 92

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 30 - June 6, 2007 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 98 98 95 95 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 98 98 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) (replicate number) 1 2 3 4 Mean Control 0.673 0.792 0.702 0.657 0.706 11.3% 0.822 0.776 0.601 0.726 0.731 22.6% 0.773 0.686 0.807 0.674 0.735 45.2% 0.708 0.770 0.663 0.810 0.738 72.6% 0.673 0.696 0.871 0.728 0.742 100.0% 0.668 0.657 0.673 0.592 0.648 Intake 0.655 0.729 0.663 0.578 0.656 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC5o: TUc = 100/IC25 5

Page 5 of 92

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 30 - June 6, 2007 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions (%Effluent) 2 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 27 31 30 28 30 31 30 30 32 30 29.9 11.3% 28 31 30 34 32 30 32 34 32 33 31.6 22.6% 34 36 35 33 31 32 37 32 31 30 33.1 45.2% 33 38 38 33 34 33 34 34 35 31 34.3 72.6% 35 38 33 36 36 37 33 35 34 35 35.2 100.0% .40 36 37 38 36 32 41 37 38 35 37.0 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/IC25 6

Page 6 of 92

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 30 - June 6, 2007 using water from Intake Percent Surviving Test (time interval used - days) Solutions SEluent 1 2 3 4 5 6 7 (% Effluent)I Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) Tet Soluetis Data (re plicate number) (% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 30 30 31 31 27 30 30 31 30 30 30.0 Intake 32 31 31 36 33 30 31 33 32 31 32.0 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC 50: TUc = 100/IC25 7

Page 7 of 92

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 30 - June 6, 2007 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 98 98 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) Test Efluentis (replicate number) (% Effluent) 1 2 3 4 Mean Control 0.676 0.614 0.686 0.682 0.665 11.3% 0.648 0.676 0.674 0.787 0.696 22.6% 0.762 0.593 0.720 0.710 0.696 45.2% 0.641 0.608 0.788 0.737 0.694 72.6% 0.621 0.734 0.542 0.616 0.628 100.0% 0.629 0.603 0.630 0.604 0.617 Intake 0.499 0.696 0.627 0.670 0.623 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LC 50: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25) Pimephales promelas May 30 - June 6, 2007 1326 7-days KC] 0.62 g/L Ceriodaphnia dubia May 08 - 15, 2007 1207 7-days NaCl 1.10 g/L 8 Page 8 of 92

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101 and Intake, May 30 - June 6, 2007. Test Sample ID Temperature( CC) Dissolved Oxgen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (prmhos/cm) (mg/L CaCO3 ) (mg/L CaCO 3) Chlorine (mng/L) Control 24.8 24.3 7.7 7.3 7.76 7.51 313 60 91 24.6 25.0 24.1 24.5 7.6 8.2 6.7 7.8 7.66 - 7.91 7.34 - 7.70 302 326 59 61 89 93 11.3% 24.8 24.3 8.0 7.4 7.74 7.47 297 1 24.6 - 25.0 24.1 24.5 7.6 8.3 6.8 7.8 7.63 - 7.83 7.33 7.70 290 310 24.8 24.5 8.0 7.3 7.71 7.46 283 22.6% Q 24.7 25.0 24.2 24.6 7.6 8.3 6.7 7.8 7.64 7.81 7.32 7.68 273 295 C4 24.8 24.4 8.0 7.3 7.66 7.45 252 45.2%/ 24.7 - 25.1 24.3 - 24.6 7.8 8.3 6.7 7.8 7.54 - 7.78 7.31 7.67 245 - 260 24.8 24.4 8.1 7.4 7.61 7.42 213 72.6% 24.7 25.1 24.2 - 24.7 7.8 8.3 6.7 7.9 7.45 - 7.76 7.27 - 7.64 208 221 24.9 24.4 8.2 7.3 7.54 7.40 175 62 72 < 0. 10 24.7 - 25.1 24.2 - 24.6 7.9 8.4 6.4 8.0 7.35 - 7.73 7.26 - 7.60 170 182 62 62 71 73 <0.10 - <0.10 Intake 24.9 24.3 8.2 7.4 7.47 7.42 175 60 74 < 0.10 24.7 25.0 24.2 - 24.5 7.8 8.4 6.7 8.0 7.23 - 7.72 7.27 - 7.60 168 181 59 60 71 75 <0.10 - <0.10 Cnrl 24.8 24.8 7.7 8.0 7.76 7.75 313 60 91 Control 24.6 24.9 24.6 25.1 7.6 7 8.2 7.8 8 8.2 7.66 7.91 7.65 7.82 302 326 59 6-1 89 93 11.3% 24.8 24.8 8.0 8.0 7.74 7.75 297 24.7 - 25.0 24.6 - 25.0 7.6 8.3 7.8 8.2 7.63 - 7.83 7.65 - 7.80 290 310 22.6% 24.8 24.8 8.0 8.0 7.71 7.74 283 24.6 - 25.0 24.5 - 24.9 7.6 8.3 7.9 8.2 7.64 - 7.81 7.65 - 7.79 273 295 4.% 24.8 24.7 8.0 8.1 7.66 7.73 252 24.6 - 25.0 24.5 - 25.0 7.8 8.3 8.0 8.2 7.54 - 7.78 7.64 7.78 245 260 24.8 24.8 8.1 8.1 7.61 7.71 213 72.6% 24.7 - 25.0 24.7 - 24.9 7.8 8.3 8.0 8.2 7.45 - 7.76 7.62 7.77 208 221 100.0% 24.9 24.7 8.2 8.0 7.54 7.70 175 62 72 < 0.10 24.6 - 25.1 24.5 25.0 7.9 8.4 8.0 8.2 7.35 - 7.73 7.61 7.76 170 182 62 62 71 73 <0.10 - <0.10 Intake 24.8 24.8 8.2 8.0 7.47 7.71 175 60 74 < 0.10 ntake _24.7 25.0 24.5 - 25.0 7.8 8.4 7.7 8.2 7.23 7.72 7.61 - 7.78 168 181 59 60 71 75 <0.10 - <0.10 Overall temperature CC) Pimephales promelas Ceriodaphnia dubia Average 24.6 24.8 Minimum 24.1 24.5 Maximum 25.1 25.1 9 Page 9 of 92

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-treated Outfall 101, May 30 - June 6, 2007. Test Sample ID Temperature ('C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Initial Final Initial Final Initial Final (jpmhos/cm) Control 24.8 24.4 7.9 7.2 7.82 7.48 305 24.7 25.0 24.2 - 24.6 7.6 8.2 6.7 7.9 7.76 - 7.90 7.34 7.64 295 318 11.3% 24.9 24.4 8.0 7.2 7.82 7.49 296 24.8 25.0 24.1 24.5 7.6 8.2 6.8 7.7 7.76 - 7.87 7.32 7.64 286 308 24.9 24.3 8.1 7.2 7.81 7.48 282 24.8 25.0 24.1 24.4 7.8 8.2 6.4 7.9 7.74 7.87 7.31 7.65 273 295

4.

24.9 24.3 8.1 7.2 7.78 7.45 253 24.7 25.1 24.2 - 24.6 7.8 8.3 6.4 8.0 7.69 7.84 7.28 - 7.65 245 263 24.9 24.4 8.2 7.2 7.74 7.44 225 72.6% 24.7 25.1 24.2 - 24.5 8.0 8.3 6.5 7.9 7.61 7.83 7.29 - 7.59 211 280 100.0% 24.9 24.4 8.2 7.4 7.69 7.43 178 24.8 25.1 24.3 24.5 8.0 8.3 6.6 7.9 7.54 7.82 7.28 - 7.58 173 188 Intake 24.9' 24.3 8.2 7.3 7.65 7.41 175 24.7 25.1 24.2 24.5 8.0 8.4 6.5 7.9 7.36 7.81 7.24 - 7.64 169 188 Overall temperature (°C) Pimephales promelas Average 24.6 Minimum 24.1 Maximum 25.1 10 Page 10 of 92

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 28-June 2, 2007, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant differences from the control during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. 11 Page 11 of 92

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None 12 Page 12 of 92

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 0 G.

5. The pH was measured by SM 4500 H+ B.

6. Conductance was measured by SM 2510 B.

7. Alkalinity was measured by SM 2320 B.

8. Total hardness was measured by SM 2340 C.

9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2.

Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. 13 Page 13 of 92

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821 -R-02-013 (October 2002).

3.

Standard Methods for the Examination of Water and Wastewater, 20th Edition, 1998.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14 Page 14 of 92

Sequoyah Nuclear Plant Biomonitoring May 30 - June 6, 2007 Appendix B Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling Page 15 of 92

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling Date March 12, 1998 - Ju Towerbrom PCL-222 mg/L TRC 11,*.*b*os la**::. mne 2, 2007 03/12/1998 03/13/1998 03/14/1998 03/15/1998 03/16/1998 03/17/1998 03/18/1998 09/08/1998 09/09/1998 09/10/1998 09/11/1998 09/12/1998 09/13/1998 09/14/1998 02/22/1999 02/23/1999 02/24/1999 02/25/1999 02/26/1999 02/27/1999 02/28/1999 08/18/1999 08/19/1999 08/20/1999 08/21/1999 08/22/1999 08/23/1999 08/24/1999 PCL-401 mg/L Copolymer 0.005 0.011 0.021 0.019 0.015 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 Cuprostat-PF mg/L Azole 0.015 0.012 0.023 0.022 0.022 0.025 0.016 0.120 2 Page 16 of 92

Table B-1 (continued). S equoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998 - June 2, 2007 Date }iiiii~ ~ iiiiiiiiiiTowerbrom :::::: :::::::::: PCL-401 i i::::Cuprott )*!* m g/L iiiiiiiiiiiii iiiiiiiiii .m g/L 4iiii~i* ii ii PF iii * !!i!

.:.::::::::::::::::Copoly m er::::::

A::::: m g/L N***!!:::~~ii

iiiiiii***i*:::::::::::,

02 0 /201/3=1/2000

== =.......=....

..=.......====

<:: 0.0:000 62 0.0 09i((((((* . 09 i~i{ii i i~~*{i*:::...:::....:.......... . 09 ii~iii{i~~i::*{i~ii~ii 0 2 0 5 2 0 0 ~i{iii(({iii{i!{i*

((*7((*(({i((i{ii!i~~iii!*i*{..

<0. 0 2...................

0. 0 9

...::::4

J:::::::::::::::

% % *4# iiiiiii~ i~ 0 2 /301/2 0 0 0 0 6 (({i((*i{!i~i 0 0 9............*............iliiii~*;!# !% !ii

!* i!! ?i.............il?

02/06/2000,:--. <. 0 2 0.00 " M0. 009:::::: : ,:::::**-::::~~~~ i~:[i~:*i~:!i

.....*::**:~ ~ii~~i{

0 1 X.. <. 0 5...~iiii~i * ~ iii~iiiiil 0 0 2 ........:.:;::. # *i???: 1 1 /2 5 /2..... ((i~~~*{iiii** iii{~i!iii~ ~~~*i 0 0 4

0:7

/::::2 7:::::/:20 0 0:::::::::::::::::: - -- " '"....iii![!:!** i~~i!!i 1 1/2 7/2 0 0 1.. . 1 7

i:::::

i * * :::::::::::i: 0 0 1 iiiii i iiii~

i:i::i:i~i

i::i!ii::

11/2 8/2 0 0 1.

i*{i((i{i{*ii!*i!**
- - - i!i****{ii!{i!

< 0.0 0 89 .{ii*!ii{ i !!iiiiilii 0.0 19 i!{* iiii{::::::::::::::::::::::::::

X::::::::::::::::::

...:: :.+..,...: :::

12 1 4 2 0 ! :i:i:i:ii::::!i~iiiiiiii~ iiliiiii((ii~iiiiiii 0 0 1 4 iiiii~iii{* ~ ~ ii~i~iiii . 2 Page 17 of 92

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 10 1) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998 - June 2, 2007 Date !iiiiiii ~ i~i~i!iilTowerbrom ::iiiiiiiiiii PCL-401 L AATýiýi ursa-ii i~i~ *

iiiii** mg/L
i!E;' *

¢ ** gL !***i**~ii P F i iii V.:iii*i iiiiii~~ii TRC iiii iii Copolymer

ý:..:DMAW-mgiL i

01/03/2002 : % %

< 0.0042 0.014 0 1 /0 4 /2 0 0 2

L

<. 0 4

*!ii*~i::**

~i**:::**

0 1/0 7/2 0 .................................... 0 0 4 i i

i:!:i 0 2 /2 4 /2 0 0 2

< 0 0 4 01/05/2002 < 0.0042

i~i~~iii!~ ~ ~iii;iii~~

. 2 i~~~ii~iiiiiiiiiiiii 02/ 6/ 0 0 .i0 . 14

ii**!~i*!~i*!iii i~ ~ iii*!i**i~*.0 3 ~ i!*~i*i~*;i........

02/24/2002 < 0 0 4 0.004 02/25/2002 < 0.004 iiii~~~iiiii 0.023 iiiiiii*iiiii:::::::::::::::::::::::::::: 02/27/2002 <;ii;;; 0.0041*;i*; 0.023

~!iiiii!iii;~**~~i;ii~**~***,..

0/2 0 ~i*i;ii* iiiiiiiiiii iiiiiiiiiii..................... 03/01/2002 4105 iii~i iii i 0.018 i ii ii iiiiiiiiil v v~v v..-v..v ~. 105/0 5/2 0 02 092

. 18ii::

1I :

-i~ii!% i* *~

-............iiiiiiiii 05/08/2002 0 0 6 i::i::i::i::* 0 iiiiii::i: 0.019 iiiii*

iiiii
- ~~~~~~~~

0:5/10

/2 0 0 2x ~~x >x::................. 0.::0::1::9:!!!?* -0 1-

- !::::::i~*:

"..:.....J~

:i

J i: : :J J J J: : :J : ?2: : :

04/09/2003~~~.. iii~ iiiiiiiiiiiiiiiiii 0 0 39

- iii:
ii*:ii:::

. 2 ~ i::i::*! l::ii:i::!i::~:iiii~:*:::::~~i: 04/0/203.................................................... <0.113 ii~i~*!~i~~i~i~i~i!!*!ii~ii~*)**!**iii~i~!*ii~i*! 0.21 ii~~ii!!!*!iSi~iiiiii

i:.

0 8 /0 4 /2 0 0 32 00 7 ... '.`iiiii~ ~ ~~~i:i !!)~~~i;i . 2 .,,,,i::::::::i::::::i:::l:::ii:::::::::

ii:i:!:ii::::iiii::ii::i:

Page 18 of 92

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998 - June 2, 2007 Date

.iiii;iii i~~~~~~ii~*

To erro b Opiii PCL-401 i.* !ii Cuprostat-N i 0 6 1 5 2 0 3 ii i i) ii } 0. 04.:............. i:::;i~

:i;i: :***:::**

.iiiii!::::: : C o p o ly m e riii;***** iiii::::::::::::::::::::::::::::::::: 106/15/2003 :::::' < 0.009045

i*i !

ii*~~;i*l . 2 ii!iiiiiiii*~~ii;!i~~*iiiii iiii 10 / 8 /2 0 3 0.0 0 6 i~ii*!iii!!;iiiiii~ i

ii~i*!i~iiliiliiliii!!;

0. 2 0 06/17/2003:4.- <0.00348

- ~~i!i~~ii~N**iiiiii*~!

0.014.... ii !:~ ii: i.......... ~i*!~ 06/18/20034...... <0 14 iiiiiii i iiiiii~i!

0. 13........*

!i! i ii~~iii ii-!i!.......~ii~i!iii*:*!iiiiiii~i

i::i~

iiiii~ ii.!.*::*.. !06/20 /20034.." < 0.00 48

I : ::::::::::::::::

0.020

I: :::

!07/0 7/2 0 04.. 0 5

I.*

0 19 Page 19 of 92

Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998 - June 2, 2007 '46 mi iii~i-* iiii mg/L iiiiii !iiii mg/L 1:~:-.. 7551 TRC ii*:.MM -6i~iýi Copolymer *iii i~{ Azole iiiii iii mg/L iiiii wiiiid:::;:: C.:***?:?::i~::

??:::****:~ ~:

i*i~~~~ii* '*:*:*i'ii~iii~ii*- 11/07/2004 < 0.0 187 iiii{**{ { !!{~(((( 0.0 1 0 2 /0 8 /2 0 0 0. 1 1 1 / 1 1 / 2 0 0 4 i!!# !*i* ! ! !i~i* < 0.0 1 4 9 0.0 1 7

..:::..:::+.:::

i:i:!:i

!:i:i) i,

i, ,1 1-.1 1. .i -~ -......... 0 6/0 /2 0 5 0 0 2 9

% ii~i~iiii~

!*i!*!i~i!

!ii!

0 2/106/2005 0.01842

.::::::::::::::::::::::::10*iiiii!!

07 1 /2 0 0 0 09 0.0 109 0.007.......

- i!~

i: ::::

ii ii:ii# * ::::{:::

07/1/2005 <0.01163 !*~sii;i{ {*;{* .0 10 3 /2 0 0 0 6 8 0 2 /0 9 /2 0 0 5 . 1 2

0:::

01 9 9:::::::::::

0.0 1 0

i:

i:{i::{: ii {:*:::(({i*:{ i i i i ! ii.................. < 0 0 4 0. 1 .........(( i ii i 0 2 / 1/2 0 5 0...... 0.0 10~iiliiii~ iiiii~iii~~ii 0.:0 07::::::::::::::::::::::::::::: 1 1 / 1 9 /2 0 0 5.. .. 0. 1 6 I.::!!i~:::ii

~~~iii::::*

- ~iii i: ? :~ii!i~::

:::... + :::

Page 20 of 92 J

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiological ly induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998 - June 2, 2007 iiiiiiiii ~ iiiiii Y webomiiii ~ iiii! PCL-401 CUPTOSii~ii: Cursa-PF -.1-30Mi Nalco iiiii il

* ~

~ ~ ~ ~ ~ ~ ~ ~ ~..... mgL

~ii~i~~~~~~~~i gL
- - ~

i* gL 35 i~

i~~~~~~

Mi::::::::::::::::..::::::::::::::::......:...::::::::::::~i! Ti ii

ii::*i~~i;:~ii~~i~ i3::ii::i*,::i*:

0. 2 6

- i::**::*i:

iii::ii~~ii~:....::..::. ::...:....:...

...............................:.:...:.:i:!:i

0.

0 5..

: : : : : : ::...... +

i~~i33~ii~~i ~il*! ii~iii*{il~iii~{!!ii~~i i*i!ii~ii!i!{!i~i{ii 0. 2 2 i

.:.::... ~i i*!* iiiiiiiiiiii

+.:+. -.......

6 ~ ~ ~ ~ ~ iii ii~i*?i~~ii 0....::..:...:::::::::.:...::::: X.::,* !ii!i~ii

.:.:.-.+

??? ??? ?? .i~~i~i ...ii!:: i**: i***::::: ~~i~~i~~i~ }!iiiiiiiiiiiiiii~i~ liiiiiiiil))iiiil iiii*~ii3iii~iii:')iiiiiiiii~iiiii 3* *3%

':!*!i~ii~ii~ii-

~ii:3i::::...:....:..ii:il!

7 ~ ~~~~ ~~ iiiiiiiii~ iiiiiiiiiiiiii -Y Iii(((({~i*::{ i!((i~;;;{**{i:{:i:{i {*.* i~ii: ((i~i~iii* i~iiii{i((iii((ii{ 0 0... i*i{:{ iii((!}iii~i~~ii*{i~iiii*ii{i((i!:ii~i~i~{ii~~ii-:i::!~i:i::i::..i:i:iii::i::i 0. 1 ~i{i:i { a i{ii !!!*!~iii!i!!!.............. 7 i*3iiiiii*ii~{!ii~ ~i{;ii*!i((~iiiiii 0 0 1 3 iii~iiii~iiiii:*(({~iiiii{!i!i :::::::.............:.:.........::::::::::::: ......~i((*i~ii~iiii~i *!{i*;{ i * {!i*;i

- i*::**::**::**::i*:**::

.:............................:...:.::....::::....::::+

Page 21 of 92

Sequoyah Nuclear Plant Biomonitoring May 30 - June 6, 2007 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 22 of 92

BIOMONITORING CHAIN OF CUSTODY RECORD Page I of I .91 Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 Delivered By (Circle One): FedEx UPS Bus Other (specifyi): Sonic Delivery Client General Comments: Collected By: Chevy Williams, Roy Quinn, Steve Williams Fax: 828-350-9368 very 05/29/07 4 I. I Sonic Deliverý. 05/29/07 (l-f l ETS 05/29/07 i-ll -1 IE-S Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I F Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 Delivered By (Circle One): FedEx UPS Bus Other (specify): Sonic Delivery Client General Comments: NPDES Number: TN0026450 Collected By: Chevy Williams, Roy Quinn, Steve Williams Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow Sample Description Number & MGD Rain Event? -9 Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear. Inches Number Temp. aiice SQN-101-TOX Comp 05/30/07-05/31/07 0751-2 (2.5gal) NA X z,] 5..c - \\ ___1_ 0651 _O~S1Z 1 U SQN-NT-TOX Comp 05/30/07-05/31/07 081 1 (2.5 gal) NA X 0711 1-1-- Sample Custody - Fill In From Top Down -4_ CA.5k W Relinquished By (Signature): Date/Time Received By (Signature): Date/Time V-.4 CheyWlim 05/31/07 10 06-. Sonic Delivery* =*,.* _./ef/*9 05/31/07 /,//* ,~- Sonic Delivery 05/31/07 ETS 05/31/07 t Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are prserved by storing them at 61C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laborator, will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arr,e until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I I. Client: TVA ~4 I. Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 FedEx UPS Bus Delivered By (Circle One): Client Other (specify): Sonic Delivery General Comments: Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Fax: 828-350-9368 Collected By: Chevy Williams, Roy Quinn, Steve Williams I P W illia Sonic Delivery -e6iotý C6o-OZ-c1 4 (-; / Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testing Solutions, Inc. Pa*e 1 of 6 Envionmnta TetingSoltios, nc.Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #:

57?4Q County: Hamilton Treatment: Non-treated Outfall: 101 Test organism information:

Test information: Organism age: 2A-AO'Yý Ot-b Randomizing template: eLLo LO Date and times organisms t Incubator number: were born between: 5 C. Organism source: Qo G.S-4e1-q Artemia lot number: b6" o07'e U Transfer bowl information: pH = Temperature = °C Total drying time: ?__-___%_ -I. FA I.,.. Date/ Time in: o0-4.-&-1 t150 Average transfer volume: Date / Time out: aw-tcn-n ao Oven temperature: Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 oS--o-- 0--, Koac xoZ-. S-6%-o1 0 -oL29.oloL 01"6 ~-j al &CO IL.J IZ)17SO OS --U-61f -w 2 3 2 Or6-c(q1 09 (k OO 1-4 I)!; Os-,zt -cf 8 01S1 oo I rL. Q5 011O nn O -- j-5 0 Iz0-S-6Zt(Q A ( Ci OZO kcA. 06 4d 0 6-a&014 r 01-4t) O'So 101 k= ~ cdio(6-C.AI L*-J 75 a0Z- 'C 61 0 01 Page 26 of 92

Environmental Testing Solutions, Inc. Page 2 of 6 Enir-en-lTstn So "ios In.Pge2o Species: Pimeph ales promnelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: OCs-BO-cr Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L 0 t 0 10 /0 0 /0 10 10 /0 /0 / 0 (0 3 CO 10 10 I O /0 10 / b /0 / c 0 '0 /0 4tO /0 /0 10 10 10 t '0 /0 /0 /0 '0 /4 10 10 /O /o (0 /0 /, /1 /0 C0 CC 4 13iO /0 I010 1 10 1O q 10 /0 /0 '0 6 10 /0 /1 0 10 10 O 1 10 Jo /0 10 /C0t /0/0 /0) C10 I o/ 0 /0) A = Pan weight (mg) Color 6 15-0 identification:,' ++.a ~.- I." i'~O *.t f,0 tq*q 1rq/t:3 Analyst: 'L B = Pan + Larvae weight (mg) Analyst: 9 jOB xaC~ko'O5 29 A00c5 200aoI rt w, 20.gA-Larvae weight (mg) = A-B Weight per initial numb* of larvae (mg) / C /. Initial number of/arvae !rof 0' V A~ A ~, 0' '0 0~ /- A 0 '.9 'V A 0~ A C)' a A\\ Average Perce it weight per redu ion initial from ýontrol 0 number of (%) larvae (mg) _______I -'. o.i*S -44Adt Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, ig = unusually la e, d&r = decanted and returned, w = wounded. Calculations and data reeviewed: Comments: t AJlL~ ~ ~ L~bJQ -c rkC.'JLTS (AGPIQ~bVQo~ Page 27 of 92

vnvironmental Testing Solutions, Inc. Page 3 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: c -zo -c-l Survival and Growth Data Day 45.2% 72.6% 100% M N 0 P Q R S T U V W x 0T /0 /1 /0 /0 // /0 to /0 10 /(0 /0 /) 1 /0 t0 I0 /0 £0 JO 0 /1 C 0 10 ( 0 10 2 /0 /U 1o t© 10 /0 /0 /1(3 /0 C 0 L 3 30 /1 C0 /00/00 '0 /0 13 // 10 '1 4 J O I C) 1c) 10 103 /0 Jo /0 /O () 1O 5 10 /0 /0 tO I 1t /0 103 10 /0 to /0 6 I 10

1) 0 iC J

/0 10 /0 C) 1I 10 1 7IM 1 *0 10 ic) 10 10 J 0 to t10 0 1C 10 A = Pan weight (mg) Color identification:Lj2,Lr* N,0 C I*"5131 dq

o1,oi,%

7 i-* -.. I.,o Analyst: B = Pan + Larvae weight (mg) Analyst: UL fl. aw R-"1 lq.Tl Ra.sq aq.q3 at. A3,adO.kda 1CI 91 Larvae weight (mg) = A - B Weight per initial number 4 A O of larvae (mg)

41)

x.

4 = C/Initial number of larvae 'N "0 Average Percent weight per reduction initial from control s.1 0.iqz t"7. C) i number of (%) larvae (mag) Comment codes: c = clear, d dead, fg fungus, k = killed, m missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data review ed: Comments: Page 28 of 92

ipnvironmental Testing Solutions, Inc. Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: os C-" Survival and Growth Data

Day 100% Intake Y

Z AA BB 0 /10 1 1 t 10 1 )0 O /0 tO 2 /0 /0 /0 10 3 /0 /0 /0 /0 40 10 /0 1(' 5 I C) 10', 10 6 (0D D /0 ) 0/ /0 to Jo A = Pan weight (mg) Color identification:* OA I39 iýýk 5hL uuL* Analyst: B

Pan + Larvae weight (mg)

Analyst: al1l 33 oil.1o0 M-I1SA 1 Larvae weight (mg) = A - B Weight per initial number of larvae (mg) t' A,9, A = C / Initial number of larvae L7 Average Percent weight per reduction initial from control ,7. number of (%) larvae (mrg) Comment codes: c clear, d = dead, fg = fungus, k = killed, m missing, sk = sick, sm= unusually small, ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 29 of 92

4 CU 0

0 0 0 0 CU 000 64I 0 r~4 ~ ~4 Cu 4.' 4-. C 4) C'u C' C' C cu C' C' C' C C C' u U 0 .1 'C U, 10 g2 C> C> o 0 0 0 0 D > 0r u 0 '4 .0 N -'4 .0 Q 0~ C "4 ~ ~'4 '4. 4):- 0, E .0 a II ~ o o 4) ~ ~..~ Co Co *'4 .0.0 a .~ .~ ~

0 *VCo 4)4)~

a '4-~o 0 -a U 0 0 (-4 C-C) '4 .0U Co 0. '4 .0 0. .0 '4-0. 0 QCo ~ ~-

4

4 4.')

4.0 4.0 Co ~ L: 0. Page 30 of 92

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/30/2007 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Outfall 101 End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.6730 0.7920 0.7020 0.6570 11.3 0.8220 0.7760 0.6010 0.7260 22.6 0.7730 0.6860 0.8070 0.6740 45.2 0.7080 0.7700 0.6630 0.8100 72.6 0.6730 0.6960 0.8710 0.7280 100 0.6680 0.6570 0.6730 0.5920 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7060 1.0000 0.7060 0.6570 0.7920 8.539 4 0.7304 1.0000 11.3 0.7313 1.0358 0.7313 0.6010 0.8220 13.029 4 -0.501 2.410 0.1215 0.7304 1.0000 22.6 0.7350 1.0411 0.7350 0.6740 0.8070 8.870 4 -0.575 2.410 0.1215 0.7304 1.0000 45.2 0.7378 1.0450 0.7378 0.6630 0.8100 8.831 4 -0.630 2.410 0.1215 0.7304 1.0000 72.6 0.7420 1.0510 0.7420 0.6730 0.8710 11.982 4 -0.714 2.410 0.1215 0.7304 1.0000 100 0.6475 0.9171 0.6475 0.5920 0.6730 5.807 4 1.160 2.410 0.1215 0.6475 0.8865 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.98098224 0.884 0.13838349 -0.4270667 Bartlett's Test indicates equal variances (p = 0.77) 2.5448122 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.12154682 0.17216263 0.00522627 0.00508725 0.43117049 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 IC10 IC15 IC20 IC25 IC40 IC50 84.671 96.741 >100 >100 >100 >100 >100 sqnO5-30-O7data

TVA / Sequoyah Nuclear Plant, Intake Non-treated May 30 - June 06, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/30/2007 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Intake End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.6730 0.7920 0.7020 0.6570 100 0.6550 0.7290 0.6630 0.5780 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7060 1.0000 0.7060 0.6570 0.7920 8.539 4 0.7060 1.0000 100 0.6563 0.9295 0.6563 0.5780 0.7290 9.419 4 1.152 1.943 0.0839

0.6563 0.9295 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94020569 0.749 0.40988519 -0.6290803 F-Test indicates equal variances (p = 0.97) 1.05143547 47.4672279 Hypothesis Test (1-tail, 0.05)

MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.0838889 0.11882281 0.00495012 0.00372746 0.29299587 1, 6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05* 70.955 IC1O >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100

indicates IC estimate less than the lowest concentration sqn_O5-30-O7data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Pimephales prom elas Daily Chemical Analyses Project number: 3240 Reviewed by: 4e Concentration Parameter Day 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Initial Final Initial Final Initial I Final Initial Final Initial Final Initial I Final Initial Final pH (SU) 7.91 7.61 7.66 7.59 7.751 7.36 7.71 7.50 7.74 734 7.79 7.48 7.75 7.70 DO (mg/L) 7.81 7.8 7.7 7.6 8.21 6.7 77 7.27 7.6 6 76 7.4 7 Cnrl Conductivity (g.mhos/cm) 326 3639315 3023031 Cnrl Alkalinity (mgfL CaCO3 ) 60 6159 Hardness (mg/L CaCO,3) 91 Temperature CC) 24.9 24.3 24.6 24.1 24.7 24.3 24.81 24.3 24.7 24.4 24.6 24,2 50 24.5 pH (SU) 7.83 7.51 7.76 7.501 7.82 7.36 7.68 7.44 7.64 7.33 7.63 7.47 7.79 770 11.3% DO (mg/L) 8.0 7.7 8.3 7.4 8.0 7.0 8.0 7.4 7.6 6.8 7.8 7.5 8.0 7.8 Conductivity (pinhos/cm) 306 291 292 290 294 310 298 Temperature CC) 25.0 24.4 24.6 24.4 24.7 24.1 24.8 24.1 24.7 24.5 24.7 24.3 24.9 24.5 pH (SU) 7.81 7.50 7.73 7.48 7.75 7.35 7.67 7.42 7.641 7.32 7.64 7.44 7.76 7.68 22.6% DO (mg/L) 8.0 7.6 8.3 7.4 8.1 7.0 8.0 7.3 7.61 6.7 7.9 7.6 8.0 7.8 Conductivity (1imhos/cm) 290 280 280 =___278 273ý 295 ý 284 ý Temperature*CC) 25.0 24.4 24.7 24.6 24.7 24.2 24.8 24.6 24.71 24.5 24.7 24.5 24.9 24.5 PH (SU) 7.78 7.48 7.71 7.48 7.67 7.35 7.63 7.42 7.54 7.31 7.61 7.44 7.67 7.67 45.2% DO (mg/L) 8.0 7.6 8.3 7.4 8.21 7.1 8.0 7.1 7.8 6.7 7.9 7.5 8.1 7.8 Conductivity (pmhos/cm) 260 250 254 250 245 253 253 Temperature CC) 25.1 24.4 24.7 24.3 24.8 24.5 24.8 24.3 24.7 24.5 24.7 24.6 24.9 24.4 pH (SU) 7.76 7.44 7.67 7.46 7.58 7.59 7.40 7.45 7.27 7.58 7.42 7.61 7.64 DO (mg/L) 8.2 7.7 8.3 7.4 8.2 7.1 8,0 7.2 7.8 6.7 8.0 7.6 8.2 7.9 72.6% Conductivity (iimhos/cm) 221 214 211 208 208 214 213 Temperature (C) 25.1 24.7 24.7 24.5 24.7 24.3 24.9 24.2 24.7 24.3 24.8 24.41 24.9 24.5 pH (SU) 7.73 7.41 7.63 7.46 7.481 7.31 7.55 7.36 7.35 7.26 7.50 7.391 7.55 7.60 DO (mg/L) 8.2 7.8 8.4 73 8.31 7.1 8.2 7.3 7.9 6.4 7.9 7.31 8.3 8.0 Conductivity (gmhos/cm) 182 177 176 172 170 173 174 100% Alkalinity (mg/L CaCO3) 62 62 62 Hardness (mg/I CaCO.3) 7 37 Total Residual Chlorine (mg/I) <01<.0<00 Temperature CC) 25.1 24.6 24.7 24.2 24.71 24.2 25.11 24.4 24.8 24.4 24.8 24.6 25.0 pH (SU) 7.72 7.46 7.62 751 7.351 7.35 7.49 7.36 7.23 7.27 7.40 7.421 7.49 DO (mgfL) 8.2 7.8 8.4 7.3 8.21 7.4 8.3 7.1 7.8 6.7 7.9 7.3 8.3 8.0 Conductivity (jtmhos/cm) 181-172-174-- 68 179 178177 100% Intake Alkalinity (mg/L CaCO3) 59 60 0 Hardness (mg/L CaCO3 ) 71 75 Total Residual Chlorine (mg/L) <0.1 0.0<0.10<0.1 _________Temperature (CC) 25.01 24.31 24.71 24.4 24.81 2.2 24.224. 2.71 24.31 24.81 24.51

2.

Environmental Testing Solutions, Inc. Page 5 of 6 rnvironmental Testing Solutions, Inc. Page 5 of 6 Species: Pimeph ales prom elas Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistry: Date: O5-o -_C7 Day 0 1 2 Analyst V", I'1-kC. Concentration Parameter CONTROL pH (S.U.) "l.q (., DO (mg/L) "1. Conductivity bz.o (4mhos/cm) Alkalinity (mg CaCO3/L) (0, 0 Hardness (mg CaCO3/L) Temperature ("C) -L- "L, i

4.

A". .z pH (S.U.) "1.&;B to b- ,]* "*-7) 9 2'1 11.3% DOL(mg/L).O 3 i t.0 ConductivityW6: (iLmhoslcm) 2c I Temperature ('C) 2L..0 N.. 1A. ILIA pH (S.U.) -1. & ý.71-t3 22.6% DO (mg/L) .03 Conductivity 0 io 00 (jimhos/cm) Temperature (°C) ?-r.O i", a 2.. pH (S.U.) -j ""--.-. awI 45.2% DO (mg.L) 830 Conductivity (jimhos/cm) Temperature (°C) .1S.1 1'4. 'zq.i L'I.* 14.& 2'4,S PHn "-..).vq q - +.q~ L.d -16*9 72.6% DO (mg/L) C. ý- Conductivity "ZL t ?I (prmhoslcm) Temperature ("C) Is.%

1.

LA -. 1 .4.1 i"q. 2* pH (S.U.) -.1-I5 -4.' "q-U3 "*.,qe5 100% DO (mg/L) .3 Conductivity (pmhos/cm) Alkalinity (mg CaCO3/L) Hardness (mg CaCO3/L) TR chlorine A,_0 10 (Mg/L) Temperature ('C) 'IS.

4.

kt. i- .2.. II ~Page 34 of I9 f 92 pH (S.U.) 1.12. II ~ I 3.LZ II )~~] I ~ 9~35 I 00% Intake DO (mp/L) 8Z7 11 1. Conductivity (ymnhos/cm) J I Alkalinity 51 (rag CaCO3/L) (0 LI I Hardness -11 (mrg CaCO3/L) TR chlorine (mrg/L)Tempeature(°C) <01 -2s.0 -zq. a 2q-,7 zq. a I Initial Fia Initial 11 Final [ Initial II /

Environmental Testing Solutions, Inc. Page 6 of 6 i~nvironmental Testing Solutions, Inc. Page 6 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: ; S-,--- Da 3 4 5 6 AnalAst lin Concen-Parameter tration CONTROL pH (S.U.) -I DO (mg/i) 77U Conductivity ) (4rmhos/cm) Alkalinity (mg CaCO3/L) Hardness (mg CaC0 3/L) Temperature ('C) -L* A. A1 IA. -VA. 2.. 115.0 pH.(S.U.) ,q .hC. 11.3% DO (mg/L) .1 Conductivity (ýlmhos/cm) i 0 Temperature (°C) "f4, *A 1...11 -1 q -zq S pH(S.U.) 22.6% DO (mg/L) Conductivity d -ý C (tIrahos/cm) e Temperature ('C) z,.& 1--1 ILI 2Fq pH (S.U.) 45.2% DO (mg/L) Conductivity 2 [19* (*mhos/cm) 1 3 pHlkalinity !9 177-e 72.6% DO (mg/L) (100%aCOs/L) Temperature ('C) 'LA. \\ -. 4 A.- li-I. Z'. 6.0 2E-2.V _pHsu. .3

1.

"*.U 4 -35 100% DO (mg/L) '-- a-i Intake Alkalinity _l ! !!/ Hardness (mg CaCO3/L) "1 TR chlorine (mg/L) )/0.10 Temperature('C) 1A &

Zq.s zs.o I

Initial 11 Final I Initial II Final I Initial II Final Initial I Final I Page 35 of92

Environmental Testing Solutions, Inc. Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project#: "5240 County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 .1.'- 1130 1815 2500 Diluent volume (mL) 2217.5 1370 685 0 Total volume (mLL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: <' "ox-j-4S 0, t Randomizing template: t.t.u4 Date and times organisms ct)qo TO 10o46 Incubator number and were born between: shelf location: 2.1 Organism Source Cups: Z, S. 6.1, 10,1 YCT batch: Culture board: OS.2"-cr-i a'4 tA,, 37... 0_____-_ Transfer bowl information: pH =".'iq Temperature = "A., 1 Selenastrum batch: oS-6-hl

O%-3\\-J1 Daily renewal information:

Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0 U s 2 1- )- c y 'l 1 I 0 o *.0T -Af l C n 0 o S 2 1.0 1 4 T -ff L. 1~ ~ T mob f' I0 OS. 29-f) n~ A "IOS-L9.o 0 *,4,.0 - J Die (A-1To005 osz.-Zt-7 6 5 1 os .OI'- _ aIO .3 1

01.

1O

o.

Il 0"Z&-l 6 C.5'. : 2 A '3 J-- -0 __o_ I_____ OS. 1-01 A 610 (o. t83 L 5 os-?, 3OS A 00o0o2.oI.ot. 6 C,-c*- 01 1 t.Zo 0S.5 1-01 A 01o-o0Z.0ovbOt. Control information: 1 2 Acceptance criteria Summary of test endpoints: % of Male Adults: 0Y 0-1. 20% 7-day LC50 ) 1001'. % Adults having 3 dBroods: W7. /'&.

i. 80%

NOEC I 067. % Mortality:

07.

07,

5 20%

LOEC > i 0(57. Mean Offspring/Female: 2-.'i 30.o0 >15.0 offspring/female ChV >1007. %CV: q97.1 3.&M < 40.0% IC25_ > 100% Page 36 of 92

Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: S-_-6 -al CONTROL - 1 Survival and Reproduction Data SI Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced 0 0 0C C 0 0 © Adult mortality I I (- [ k. Young produced 0 c_ C_ 0 ) Adult mortality I-I 2 1 3 Young produced Id 0 co 0 _ Adult mortality l-I II'---]t--I - -I It H-Adult mortality \\- I.._ I C. I 5 Young produced 1 to [ i t Ii IL I1 Ct) Cic:, T+/-* +/- __inal I__Adult mortality I.- I.I 6 f Young produced TC) 1 L " o1 0 1o) _o7 Adult mortality ( L =

live, D = dea)I 7

1 Young produced t3 ~,L'A

.+/-..tS iU I-I Total young produced Z

-I A 30 3 O C 37! Final Adult Mortality [ -i:---CI:- -I i. Xfor 3rId Broods Note: Adult mortality (L live, D =dead) Concentration: % Mortality: I 07 j Mean Offsprin emale: Z"%* j CONC: 11.3% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 SAdult mortality L U IL-L- L U U 2 Young producedT qo 6 1 61 6 [c1) ] AIAdult mortality I C l \\ l.[ Y I U1 i Z i j L. 3 Young produced Co[ 0Io O 0To o ~ 1 Adult mortality L_ U I. 4 Young produced L_ 1_ 7_5 _5_ Adult mortality I K-K K "K K..,- 5 Young produced 10 I()I C '_1ft... LI [ L-) Adult mortality Young produced rII lj j I ',o J Total young produced ZT a t 3 3 2. 3 o 3 ý 3 a I-33 Final Adult Mortality \\ I,. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 07. Mean Offspring/Female: 31.. % Reduction from Control: -S.I17. Page 37 of 92

Environmental Testing Solutions, Inc. Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date: 0S-5-2--61 I CONc: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 I 8 9 10 1 Young produced O Adult mortality L L.. Adult mortality I L_ I* I \\.-k -I i-I t I_ 3 Young produced C01_o c o )jo 0 C) Adult mortality ,-I \\- L I ,,, 74 Young produced LA* ý-A l* l' A l-k' t:* l* I AduI mortality 1 Young produced [ 1 [ {* ioj3iAJ Z7-- Adult mortality ie, D = da__ [Youngoproduced Cr ac Adl mortrlityt\\- t"7 - [ Young produced 1 19 1 L. r ) Total young produtced 34 36 ZS 32s 3~ 137 .3-1 37-31 3 0 Final Adult Mortality A-.,JL-I Note: Adult mortality (L live, D dead) Concentration: Mortality: 0d 1 Mean Offspring/Female: !) %Reduction from Control: 1.T? 5 COWn: 45.2% Survival and Reproduction Data T ______ ______Replicate number D 5Adult mortali 819 10 3 Youngproduced O 0 [ o.- IQ] C).f0]OVC Adult mortality -..a..- 4 Young produced C, C) C) l) c) I Adult mortality 3 Young produced hu"_ l i) 3 II21 "2-tf Lq 1k "--___al Adult mortality j I k._._ 6 Young produced 0'A }Adult mortality tJUfJ-L ] U U dl otlt ý -I\\,I -IL I L-, IýI I L-L- S 7 Young produced 120 l9 19 16 1 i t.I Total yountgproduced

5 331 Lk~

3 %A -':5* tFinal Adult M4ortality ~ t-- k-iI. (- I. C Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: OX Mean Offspring/Female: 3q.3 % Reduction from Control: iq"17. Page 38 of 92

Environmental Testing Solutions, Inc. Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: o-.5--2)O-r-I CONC: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 i Young produced -) © 0 © C C r5 I Adult mortality \\ j 2 Young produced C) C L Adult mortality 1 zi L]L.]L*[I\\.1L*1

I_

4 Young produced __I

°"*Adult mortalityI1l I I

I L "'1 5 Young produced 12 12-'L 1..Lj2-L v2~gcIt 2-IIz Adult mortality k k[ l`- %,- I 6 Young produced jCj o J [ 0101 C) I ~ 01 Adult mortality I P-I 1 7 Young produced 1 T10 1 -9 Total young produced Y 5 1 3S ZIA Final Adult Mortality [

t.

\\... \\....- %i... L.. I Note: Adult mortality (L live, D = dead) Concentration: % Mortality: 07,. Mean Offspring/Female: 34.2*, % Reduction from Control: ,ii'.7. CONC: 100% Survival and Reproduction Data Replicate number DaF 1 2 3 _4 6 7 8 9 10 1 Young produced C)c mm 010.0 Adult mortality 2 Youngproduced IC) 0 C CoI i sjo)oo Adult mortality (._-- L -- 'L 3 Young produced C) C) L* C) C_). ,c[0I,,1q[5 Adult mortality L-L - L - L-4 Young produced _1

I C jm{_°*1[-H-e+&+'-

{ Adult mortality \\-- 7 Young produced 7-_ _4 13 13 17- {= Adult mortality L-___ k___ Young produced JL C)[ 0 ~ (~+/-L~-_ +/-~:I2 Adult mortality ~[-LI-6 ouYoung produced i -L]ol i 0 _Ic_[ Io_ Iq Total young produced qo 3, 31

3.

3!a '4 l 31 5& 3$ Final Adult Mortality i \\... j X_..- } M.. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 01. Mean Offspring/Female: A31. 0 % Reduction from Control: -2S."17, Page 39 of 92

Environmental Testing Solutions, Inc. Page 5 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date: CONTROL - 2 Survival and Reproduction Data Replicate number Da*, 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 ( Adult mortality I L-- I %-- I f { 2 Young produced r-C ) t* ol) 1C-, ) ~ i Adult mortality L --I \\'-I -- I k 1 - -" -'1*- 3 Young produced 0 0 ( Adult mortality Young produced __&A K Adult mortality u s,_ tL.. Uit-"J Adult mortality ' I j 6{Young produced __o~ c c~ C C) c c) ODlc Adult mortality 1,-I \\-Iý- -"-I --I ý-~ I 7 Young produced I LA I'S k \\{ I } L I-- I-Total young produced 3 1.1 3" 5 Final A dult M ortality \\... k._.. \\_. k...... k.._

Note: Adult mortality (L = live, D = dead)

Concentration: % Mortality: 017. Mean OffspringFemale: .-30.0 CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1 12 3 4 5 6 7 8 9 10 [aYoung produced C) 0 ) 0 0 ') 0 0C 0 _ _ I ] d0 J 7 1]& [ ia]Young produced _. Adult mortality I_._ L_ t 3 Young produced C_ (2 C Adult mortality Q I 4 Young produced Adult mortality LJ [ I\\-- I I u I 6 Young produced o Q ] J0 1O ] o J o Adult mortality -I I X-- L L Lj L] U--IU 7 Young produced I 1 Total young produced 3 2 31 51 3 (- 5 3 ,r 1 3 1 3 z-Z Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: pT. Mean Offspring/Female: 132. 0 % Reduction from Control: -6,.7l. 1 Page 40 of 92

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06, 2007 Verification of Ceriodaphnia Reproduction Totals Control-1 DyReplicate numlber Total y 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 r4 5 4 3 4 4 4 5 4 42 5 10 11 11 10 12 10 10 10 10 11 105 6 0 0 0 0 0 0 0 0 0 0 0 7 13 15 14 14 15 17 16 16 17 15 152 Total 27 31 30 28 30 31 30 30 32 30 29 11.3% Day ~1 2 Re licate number y 1 2 13 4 1 5 1 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 5 4 5 4 5 5 45 5 10 10 11 10 11 10 11 13 11 10 107 6 0 00 0 0 0 0 0 0 0 0 7 14 17 15 19 16 16 16 17 16 18 164 Total 28 31 30 34 32 30 32 34 32 33 316 22.6% Day I Replicate number Total 1 2 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 6 4 4 6 4 5 4 47 5 12 13 13 12 10 10 14 12 11 10 117 6 0 0 0 0 0 0 0 0 0 0 0 7 17 18 18 15 17 18 17 16 15 16 167 Total 34 36 35 33 31 32 37 32 31 30 331 45.2% Day Re licate lnmber 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2-0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 6 6 4 5 4 5 5 6 4 49 5 11 13 13 10 13 12 12 10 11 11 116 6 0 0 0 0 0 0 0 0 0 0 0 7 18 19 19 19 16 17 17 19 18 16 178 Total 33 38 38 33 34 33 34 34 35 31 343 72.6% Rep licate miunter Day R ee -- nu-ber Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 7 6 4 5 6 4 5 5 4 5 51 5 11 12 12 12 11 12 10 11 12 12 115 6 0 0 0 0 0 0 0 0 0 0 0 7 17 20 17 19 19 21 18 19 18 18 186 Total 35 38 33 36 6 37 33 35 34 35 352 100% Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 5 5 5 5 4 5 6 6 4 51 5 13 13 12 14 10 11 14 13 13 12 125 6 0 0 0 0 0 0 0 0 0 0 0 7 21 18 20 19 21 17 22 18 19 19 194 Total 40 36 37 38 36 32 41 37 38 35 370 Control-2 Re licate number Dy 17 2 13 4 15 16 7 8 1 9 1 0 Toa 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 5 5 4 4 4 5 4 4 44 5 12 10 11 11 10 10 10 12 11 10 107 6 0 0 0 0 0 0 0 0 0 0 0 7 14 15 15 15 13 16 16 14 15 16 149 Total 30 30 31 31 27 30 30 31 30 30 300 100% Intake Replicate number Total Day 1 2 3 4 5 6 7 8 09 110 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 5 4 6 4 5 5 46 5 10 13 10 13 12 10 10 12 12 11 113 6 0 0 0 0 0 0 0 0 0 0 0 7 18 14 17 18 16 16 15 17 15 15 161 Total 32 31 31 36 33 30 31 33 32 31 320

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 3240 Reveiwed by: Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (%) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) pooled controls (%) Control - 1 27 31 30 28 30 31 30 30 32 30 100 29.9 4.8 Not applicable 11.3% 28 31 30 34 32 30 32 34 32 33 100 31.6 6.0 -5.7 22.6% 34 36 35 33 31 32 37 32 31 30 100 33.1 7.0 -10.7 45.2% 33 38 38 33 34 33 34 34 35 31 100 34.3 6.5 -14.7 72.6% 35 38 33 36 36 37 33 35 34 35 100 35.2 4.6 -17.7 100% 40 36 37 38 36 32 41 37 38 35 100 37.0 6.9 -23.7 Control - 2 30 30 31 31 27 30 30 31 30 30 100 30.0 3.8 Not applicable 100% Intake 32 31 31 36 33 30 31 33 32 31 100 32.0 5.3 -6.7 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: MSD = PMSD= 2.092 7.0 1.127 3.8 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA ( 10th percentile) = 13%. Upper PMSD bound determined by USEPA (90'h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes l and 2-Appendix. EPA-821-B-01-004 and EPA-821 -B-01-005. US Environmental Protection Agency, Cincinnati, OH.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/30/2007 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821 -R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 27.000 31.000 30.000 28.000 30.000 31.000 30.000 30.000 32.000 30.000 11.3 28.000 31.000 30.000 34.000 32.000 30.000 32.000 34.000 32.000 33.000 22.6 34.000 36.000 35.000 33.000 31.000 32.000 37.000 32.000 31.000 30.000 45.2 33.000 38.000 38.000 33.000 34.000 33.000 34.000 34.000 35.000 31.000 72.6 35.000 38.000 33.000 36.000 36.000 37.000 33.000 35.000 34.000 35.000 100 40.000 36.000 37.000 38.000 36.000 32.000 41.000 37.000 38.000 35.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 29.900 1.0000 29.900 27.000 32.000 4.847 10 33.517 1.0000 11.3 31.600 1.0569 31.600 28.000 34.000 6.004 10 -1.859 2.287 2.092 33.517 1.0000 22.6 33.100 1.1070 33.100 30.000 37.000 7.042 10 -3.498 2.287 2.092 33.517 1.0000 45.2 34.300 1.1472 34.300 31.000 38.000 6.454 10 -4.810 2.287 2.092 33.517 1.0000 72.6 35.200 1.1773 35.200 33.000 38.000 4.600 10 -5.794 2.287 2.092 33.517 1.0000 100 37.000 1.2375 37.000 32.000 41.000 6.861 10 -7.762 2.287 2.092 33.517 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.6246798 1.035 0.04521495 -0.0695896 Bartlett's Test indicates equal variances (p = 0.56) 3.92323184 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Pmb df Dunnett'sTest 100 >100 1 2.09160219 0.06995325 65.0166667 4.18333333 1.9E-09 5,54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL Skew IC05 >100 IC>10 IC 15 IC20 1C25 1C40 IC50 >100 >100 >100 >100 >100 >100 sqn_05-30-07data

TVA / Sequoyah Nuclear Plant, Intake Non-treated May 30 - June 06, 2007 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/30/2007 Test ID: CdFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Intake End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-82 I-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 30.000 30.000 31.000 31.000 27.000 30.000 30.000 31.000 30.000 30.000 100 32.000 31.000 31.000 36.000 33.000 30.000 31.000 33.000 32.000 31.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% - N t-Stat Critical MSD Mean N-Mean D-Control 30.000 1.0000 30.000 27.000 31.000 3.849 10 31.000 1.0000 100 32.000 1.0667 32.000 30.000 36.000 5.311 10 -3.078 1.734 1.127 31.000 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distnbution (p > 0.01) 0.88865483 0.868 0.62026911 2.99845201 F-Test indicates equal variances (p = 0.26) 2.16666675 6.54108953 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.12677077 0.03755903 20 2.11111111 0.00648469 1, 18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL Skew IC05 IC10 IC15 IC20 IC25 IC40 IC50 >100 >100 >100 >100 >100 >100 >100 sqnO5-30-O7data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 30 - June 06,2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses Project number: 3240 Reviewed by:

Concentration Parameter Da 0 Day 1 Da 2 Day 3 Da 4 Day 5 Day 6 Initial I

Final Initial Final Initial Final Initial Final Initial Fin Initial Final Initial Final pH (SU) 7.91 7.76 7.66 7.80 7.75 7.78 7.71 7.73 7.74 7.65 7.79 7.71 7.75 7.82 DO (mg/L) 7.8 8.0 7.7 8.1 8.2 8.2 7.7 7.8 7.6 7.8 7.6 8.0 7.6 8.0 Conductivity (mhos/cm) 326 316 309 35302 Control Alkalinity (mgfL CaCO3) 606 59 Hardness (mg9L CaC 3)3 89 _Temperature (CC) 24.9 24.6 24.61 24.9 24.7 24.8 24.8 25.1 24.6 24.8 24.8 24.6 24.9 24.7 pH (SU) 7.83 7.77 7.76 7.80 7.82 7.78 7.68 7.74 7.64 7.65 7.63 7.71 7.79 7.78 DO (mg/L) 8.0 8.0 8.3 8.1 8.0 8.2 8.0 7.8 7.6 7.9 7.8 8.0 8.0 8.0 11.3% Conductivity (pmhos/cm) 306 291 292 290 27294 ý3 10 298908 Temperature (OC) 25.0 24.8 24.7 24.6 24.7 24.8 24.8 25.0 24.7 24.8 24.8 24.9 25.0 24.9 pH (SU) 7.81 7.77 7.73 7.79 7.75 7.77 .7.67 7.74 7.64 7.65 7.64 7.70 7.76 7.77 DO (mg/L) 8.0 8.0 8.3 8.2 8.1 8.2 8.0, 7.9 7.6 7.9 7.9 8.0 8.0, 7.9 22.6% Conductivity (gsmhos/cm) 290 280 280 278 273 295 284 Temperature (C) 25.0 24.5 24.6 24.7 24.7 24.7 24.8 24.7 24.8 24.9 25.0 24 pH (SU) 7.78 7.75 7.71 7.78 7.67 7.76 7.63 7.74 7.54 7.61 7.70 7.67 7 DO (mgWL) 8.0 8.0 8.3 8.2 8.2 8.2 8.0 8.0 7.8 7.9. 8.1 8.1 45.2% Conductivity (jsmhos/cm) 260 250 254 250 245 253 253 Temperature (OC) 25.0 24.5 24.6 24.9 24.7 24.6 24.8 24.7 24.7 24.7 24.8 25.0 25.0 24.8 PH (SU) 7.76 7.72 7.67 7.77 7.58 7.76 7.59 7.72 7.45 7.62 7.58 7.68 7.61 7.72 DO (mWL) 8.2 8.0 8.3 8.1 8.2 8.2 8.0 8.0 7.8 8.0 8.0 8.1 8.2 8.0 72.6% Conductivity (Imhos/cm) 221 214 211 208 208 214 213 Temperature CC) 25.0 24.7 24.7 24.9 24.7 24.9 24.9 24.9 24.7 24.9 24.8 24.8 25.0 24.8 pH (SU) 7.73 7.72 7.63 7.76 7.48 7.74 7.55 7.71 7.35 7.61 7.50 7.67 7.55 7.69 8.2 8.0 8.4 8.3 8.2 8.2 8.0 7.9 8.0 7.9 8.1, 8.3 8.0 Conductivity (pmhos/cm) 182 177 17 1 100% Alkalinity (mg/L CaCO3) 6 66 Hardness (mgIL CaCO3) 7 77 Total Residual Chlorine (mg/L) <0.10 Temperature (C) 25.1 24.5 24.7 24.6 24.6 24.6 25.1 24.8 24.8 24.7 24.8 24.9 25.0 25.0 pH (SU) 7.72 7.73 7.62 7.78 35 7.75 7.49 7.7 7.23 7.61 7.40 7.67 DO (mg/L) 8.2 8.1 8.4 8.0 8.2 8.2 8.3 7.8 7.8 7.87.9 8.1 8.3 77 Conductivity (jsmhos/em) 181 172 174 168l179 178 172 100% Intake Alkalinity (mg/L CaCO 3 ) 5 60 60 Hardness (mgIL CaCO 3) 71 75 Total Residual Chlorine (mg/L) <0.10 <0.10 ITemperature (OC) 25.0 24.5 24.71 24.8 24.8 24.8 24.9 25.0 24.7 24.9 24.8 24.7 25.01 24.8

Environmental Testing Solutions, Inc. Page 6 of 7 Environmental Testing Solutions. Inc. Page 6 of? Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Dailv Chemistry: Date: 0S-_i0-q7 Analyst VZ~& F-j fkA f'j Concentration Parameter CONTROL pH (S.U.) I..- ,? Conductivity (1.mhos/cm) Alkalinity (mg CaCO/L) ., 0 Hardness Oil (mg CaCO3/L) Temperature (C) 2Z .9 i 0 ,( 1A -L. -I.7 2 1.% DO (mg/L) 00f o 02 Conductivity .*.ýb, ly A~ (ýrimhos/cm) a Ternerature('C.- 22.6% DO (mg.L) L_.0__ 2-Conductivity ('mhos/cm) Aqo 9 6C) Temperature(0 C) .S. .S ,4.(a L.'. IA.1 pH (S.U.) 45.2% DO (mg/L) 6, .l Conductivity (Tmhos/c ) m t 0 AGO Vý SO Temperature ('C) "15.0 z - ý.~ ~ pH (S.U.)

11. J V~l 9ý q9-7 i.

72.6% DO (mg/L) 0.2 0.3O 6.2 O'imhos/cm) All~ Temperature ('C) 'LS. LI. 2.- 49 DH (S.U.) 1 q-42 I -I-, I '71*Th 'I.

)q*AI 4 -'----'--~---4I I

I H-100% DO (m2/L) M. 2 Conductivity (4rmhos/cm) Alkalinity __mg CaCO 3/L) G__ Hardness (mg CaC, 3/L) TR chlorine <0, I0 (mg/L)____ E3. .o.I C, Temperature (°C) zS.I I.0.- oH (S.U.) ,ýz1 -1,3 1 1-1~Z II'm9( 1.9,3-I1 100% Intake DO (m-/L) Conductivity _(pLrhos/cm) Alkalinity (mg CaCO5IL) Hardness (mg CaCO3/L) 7 TR chlorine K0. 1 -(Mg/L) I_ I '~ ~ iS ~iC i~ ?'f. & iI 'A Temnerature (OC) a2' Tern erature (T) -2c. 0 Initial 1I Final I Initial II Final I Initial 11 Final I

Page 46 of 92

Environmental Testing Solutions, Inc. Page 7 of 7 Species: Ceriodaphnia dubia Client: Seequoyah Nuclear Plant - Non-treated Date: o-s-o-cl Da 3a4 5 Analyst 'b-k-VhC 1 5 Concen-Parameter tration CONTROL PH(S.U.) 1 DO (mg/L)L' Conductivity (ýiMhoslcm) 3 ) Alkalinity (mg CaCO./L) Hardness (mg CaCO3/L) Temperature (,C) Z.\\ z-L%. U -*'L.,l 2 ,'W1 pH(S.U.) 4-* 11.3% DO (mg/L) G Conductivity ýSD Temperature (°C) "t.* q.* o 2.." "1.'4..l "4.& tL "S.*O 2 42.6% DO mk)"--q (_Vrhos/cm) Temperature (°C) "-A. t 0, ..0 zA-' Ul. i.'I. ".s.o 42.6% DO (mg/L) 6.- Conductivity 72.6% DOsmg/) (5 H~Q S Temperature (°C) z.1, 9 7A.1-q.* .LS -0 PH (S.U.) 9-LS6 / I0 -q,-, -hinl v-+ Conductivityl 70.% DO (mgC L) e 0.. Hardness (mra CaCOs/L) 3 TR Chlorine m Temperature(OC) l 2I.0 PH (S.U.) '4 ý ý 1 11 q. q'1 I 'ý -A-ý) 11 -q /,1 1 -4. 'JO 11 '+ IQ -:ý I -*ýAq 11 66) 1 100% Intake Conductivity Hardness ým._gCaCO3/L) JR chlorine (m-.q TemperatuAre('C) .1 Ls L-l t. 8.3 -i-i- --- I I I Z~.5. 1124 ,,Initial 1 a Final In ,itial IF-1n TJ nitial Final Initia Page 47 of 92

Environmental Testing Solutions, Inc. Page I of 6 EnirnenalTstngSluios Ic.Pge1Mf Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #: 3BIAO County: Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: -o.'2e tAoj&. ok-b Randomizing template: t]Lt#L Date and times organisms O s Incubator number: were born between: Organism source: Ans. bAov'qvk Pe OS-<*-kCq-Artemia lot number: 8(zOqw ) Transfer bowl information: pH = Temperature = °C Total drying time: 7.4-oO*e -ZIA. 3 Date / Time in: 06-ej-D' ib-N Average transfer volume: Date/ Time out: 06-0 -dJ 150 Oven temperature: locY Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 -eo A 1osvO-.ol cjT.- I o - Bl-o 'l 40 o 0 I At )Q 1 3 0 41 o s-z e - al A 0' 0 Ao%"zo 4 a z? 2 _G_ 6qI O.- oL.p-?O -al 8: o n o \\,SW rz "- 3 4 to-'fb-' a 0Oq1(D \\I5c* IL.ZR OS-s\\-0" A cft oO..O-4. o 5 o;-C..o_ \\657-- ('*'a °"1 Z "a"oion o O°L' 6 4sr 61 04M I 21 7R 1 os-& -criA no(.c o .o% "CrL 7 Control information: Acceptance criteria Summary of test endpoints: % Mortality: C.<_ 20% 7-day LC50 Average weight per initial larvae: 0 -. S NOEC Average weight per surviving larvae:. O. 'o6 S 0.25 mg/larvae LOEC 0oo7. ChV IC 5 1 > i 0'o7. Page 48 of 92

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimeph ales promelas Client: TVA f Sequoyah Nuclear Plant - UV-treated Date: OS-&cT-Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L 0 10 10 1C) to /0-10 00 t 0 to to tc) 1 /D R) / 0 C C 10 /1 o0 IC 0 C 2 /0 t 10 10 /0 t D /0 D0 (o 10 to IC 3 tI? tO t /0 tO /I to (0 (0 /0 o 0 lo 4 10 t0 10 to (0 1 C) 10 to I C) 10 to t o010 /0 dC) /0 /:? to //0 to '0 t/0 6 / to0 0 /0 t0 10 1O( 01 ID 1010 1 I0 7 _l to /o 10 10 /0 o to 1 0 1o 1O 10 A = Pan weight (mg) Color identification:

.____* t -

1,.4 i A '73i.7 143.'1 15,75-..o 4o ý A,55 1J.;k,45 [145 Analyst: B = Pan + Larvae weight (mg) Analyst: LAQ3 A1.11 AAa2 A.i ij.19 ?11~4 ~.B* X1 .Y1 (P% d~~ Larvae weight (mg) = A - B .q t,.t t,,.gtl L0.S2 i7. 2. 6.*l.4*,a 1,tI 7.(,,Z 5.A' "1."Z "/,l Weight per initial number of larvae (mg) = C / Initial number of larvae S. '4" '9 q~V "0 "S A. 'S A 0* A. v~. A-O" 01 01 Average Percent weight per reduction initial from control

0. (,,06S number of

(%) larvae (mg) I. - i - d - 60,11,

0. 0 6 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewedk-Comments: Page 49 WN -. b

Environmental Testina Solutions, Inc. Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: oS c-c4l-c Survival and Growth Data Day 45.2% 72.6% 100% __I N 0 P Q R S T U V W X 0 10 /D /0 /0 10 /0 /D 10 i0 10 /0 1O 1 ID tO 10 10 /0 0 /0 /0 10 i0 to '0 2 0 10 /0 /0 10 /0 /0 /0 D0 /0 /0 /3 /0 /0 /0 /0 /0 /0 /0 '3 /0 /0 /0 0/ 10D 1O 1C /0 (0 0 /0 10 /0 /0 /5 /01 / to 00 /O /00 T O /0 to 6 t /C /t // 1 0 / /0 /0 (O /0 /0 7 Ib I~l It.-t /0 10 10 to 10-0 // iL) 1C 10 1 t(0 A = Pan weight (mg) Color identification: Analys: B = Pan + Larvae weight (rag) Analyst: q 10 A -Cal *.Wa A3.0o a".(1 At.-1o JONO .4 3 'oAt -k I tl9.'03 ICI.49 Larvae weight (nag) = A - B Weight per initial number of larvae (mg) N 0' =C / Initial number of larvae \\0 ,O P Average Percent weight per reduction initial from control

0. vi~q

--"47 ,9~ 6). C,/ .*o 0 o1*1 number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 50 of 92

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: C75 O"7 Survival and Growth Data Day 100% Intake Y Z AA BB 0 /0 1o to /0 I / / '0 /0 /0 2 1b 1 [) /0 3 30 t0 /0 /0 4 ___I()___ / t 0 10 10 5O CO 10 /0 6 10 /0 10 1C 7 T5-K 10 10 10C IC) A = Pan weight (mg) Color identification: qn -I.st-B = Pan + Larvae weight (rag) Analyst: _ LAq.B A.0a I8.M a.I Larvae weight (mg) = A - B Weight per initial number of larvae (mg) M A = C / Initial number of larvae \\) 0

,O 10" 1 tr Average Percent weight per reduction initial from control

0. 61

,7 number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: Page 51 of 92

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 30 - June 06, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales prom elas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwed Project number: 3240 Not for Compliance Assessment, Internal Laboratory QC Concentratlion (% Replicate Initial number of Final number of A - Pan weight B - Pan

Larvae Larvae weight (nag)

Weight I Surviving Mean wenightt / Cencent orvarit tlon Weight I Initial number Mean Iurvival Mean 1eightM of Percent reduction from larvae larvae Srog) weight (rag) A-B number of larvae (Smg) Surviving number of (-.t. pr.,,,k n of lIarva. (mg) (%) Initial number of variation crn.av.. cotrol 5%) larvae I. mg) -aum o) (%) larvae (Mug) ,,r oeS nnvM,. * -a) A 10 10 15.35 22.11 6.76 0.676 0.676 Control B 10 10 14.19 20.33 6.14 0.614 0665 5.1 0.614 C 10 10 14.73 21.59 6.86 0.686 0.606 100.0 0.665 5.1 Not applicable D 10 10 14.34 21.16 6.82 0.682 0.682 E 10 10 15.75 22.23 6.48 0.648 0.648 11.3% F 10 10 13.03 19.79 6.76 0.676 0.718 14.6 0.676 97.5 0.696 8.9 -4.8 G 10 10 14.81 21.55 6.74 0.674 0.674 H t0 9 14.55 22.42 7.87 0.874 0.787 I 10 10 14.25 21.87 7.62 0.762 0.762 22.6% J 10 10 14.84 20.77 5.93 0.593 0.593 K 10 10 14.38 21.58 7.20 0.720 0.720 L 10 10 14.66 21.76 7.10 0.710 0.710 M 10 10 13.29 19.70 6.41 0.641 0.641 45.2% N t0 10 14.42 20.50 6.08 0.608 0.694 12.0 0.608 100.0 0.694 12.0 -4.4 O 10 10 14.24 22.12 7.88 0.788 0.788 P 10 10 15.64 23.01 7.37 0.737 0.737 1 10 10 14.75 20.96 6.21 0.621 0.62 I 72.6%R 10 10 14.36 21.70 7.34 0.734 0.628 12.6 0.734 100.0 0.628 12.6 5.5 S 10 10 13.75 19.17 5.42 0.542 0.542 T 10 10 14.40 20.56 6.16 0.616 0.616 U 10 10 14.14 20.43 6.29 0.629 0.629 100% V 10 10 15.08 21.11 6.03 0.603 0.617 2.4 0.603 100.0 0.617 2.4 7.2 W 10 10 13.53 19.83 6.30 0.630 0.630 X 10 10 13.95 19.99 6.04 0.604 0.604 Y 10 10 14.13 19.12 4.99 0.499 0.499 100% Intake Z 10 10 14.06 21.02 6.96 0.696 0.623 14.0 0.696 100.0 0.623 14.0 6.2 AA 10 I0 12.62 18.89 6.27 0.627 0.627 BB a 10 10 15.71 22.41 6.70 0.670 0.670 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 0.1071 16.1 0.0911 13.7 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were determined from dse l0th and 90th percentile, respectively, of PMSD data from EPA's WET lnterlaboratory Variability Study (USEPA, 2001 a; USEPA, 2001b). USEPA. 2001 a, 200tb. Final Report: Interlaboratosy Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 30 - June 06, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/30/2007 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6760 0.6140 0.6860 0.6820 11.3 0.6480 0.6760 0.6740 0.7870 22.6 0.7620 0.5930 0.7200 0.7100 45.2 0.6410 0.6080 0.7880 0.7370 72.6 0.6210 0.7340 0.5420 0.6160 100 0.6290 0.6030 0.6300 0.6040 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6645 1.0000 0.6645 0.6140 0.6860 5.104 4 0.6876 1.0000 11.3 0.6963 1.0478 0.6963 0.6480 0.7870 8.880 4 -0.715 2.410 0.1071 0.6876 1.0000 22.6 0.6963 1.0478 0.6963 0.5930 0.7620 10.402 4 -0.715 2.410 0.1071 0.6876 1.0000 45.2 0.6935 1.0436 0.6935 0.6080 0.7880 12.032 4 -0.653 2.410 0.1071 0.6876 1.0000 72.6 0.6283 0.9454 0.6283 0.5420 0.7340 12.609 4 0.816 2.410 0.1071 0.6283 0.9137 100 0.6165 0.9278 0.6165 0.6030 0.6300 2.437 4 1.080 2.410 0.1071 0.6165 0.8966 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96499479 0.884 0.11010883 -0.2509188 Bartlett's Test indicates equal variances (p = 0.19) 7.4650507 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.10706301 0.16111815 0.00517108 0.00394707 0.30368891 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 61.066 14.544 0.000 99.872 -0.2097 IC10 IC15

IC20 IC25 IC40 IC50 94.491

>100 >100 >100 >100 >100 sqnO05-30-O 7data

TVA / Sequoyah Nuclear Plant, Intake UV-treated May 30 - June 06, 2007 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/30/2007 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant, Outfall 101 End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6760 0.6140 0.6860 0.6820 100 0.4990 0.6960 0.6270 0.6700 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6645 1.0000 0.6645 0.6140 0.6860 5.104 4 0.6645 1.0000 100 0.6230 0.9375 0.6230 0.4990 0.6960 14.033 4 0.885 1.943 0.0911 0.6230 0.9375 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.89268976 0.749 -1.2560788 1.77523149 F-Test indicates equal variances (p = 0.15) 6.64445066 47.4672279 Hypothesis Test (1-tail, 0.05). MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.09111043 0.13711126 0.0034445 0.00439683 0.41016689 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05* 80.060 IC1o >100 IC15 >100 IC20 >100 IC25 >100 IC40 >100 IC50 >100

indicates IC estimate less than the lowest concentration sqn_05-30-07data

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May30 - June 06, 2007 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 3240 Reviewed by:r-Concentration Parameter Da 0 Day 1 Da 2 Day 3 Day 4 Day 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.87 7.55 7.83 7.52 7.90 7.41 7.83 7.42 7.81 7.34 7.76 7.50 7.76 7.64 DO (mg/L) 8.0 7.7 7.9 7.2 7.6 7.2 8.2 6.7 7.9 6.7 7.9 7.3 8.1 7.9 Control Conductivity (ftmhos/cm) 318 306 301 305 295 302 305 Temperature (°C) 25.0 24.4 24.7 24.2 24.7 24.3 24.9 24.6 24.7 24.4 24.9 24.6 25.0 24.5 pH (SU) 7.87 7.62 7.83 7.52 7.87 7.40 7.84 7.40 7.80 7.32 7.76 7.50 7.76 7.64 DO (mg/L) 8.1 7.7 8.2 7.1 7.6 6.9 8.2 6.8 7.9 6.8 7.9 7.2 8.1 7.7 11.3% Conductivity (pmhos/cm) 308 300 291 295 286 292 299 Temperature (C) P 25.0 24.4 24.8 24.1 24.8 24.3 24.9 24.4 24.8 24.5 24.9 24.4 25.0 24.5 pH (SU) 7.87 7.62 7.84 7.51 7.82 7.40 7.84 7.40 7.74 7.31 7.77 7.47 7.77 7.65 22.6% DO (mg/L) 8.2 7.8 8.2 7.0 7.8 6.9 8.2 6.9 8.0 6.4 8.0 7.2 8.1 7.9 Conductivity (pmhos/cm) 295 283 278 279 273 278 288 Temperature (C) 25.0 24.2 24.8 24.3 24.8 24.1 24.9 24.2 24.8 24.4 24.9 24.4 25.0 24.3 pH (SU) 7.84 7.56 7.83 7.48 7.78 7.39 7.83 7.34 7.69 7.28 7.76 7.44 7.75 7.65 DO (mg/L) 8.3 7.9 8.2 7.1 7.8 7.0 8.3 6.9 8.1 6.4 8.0 7.1 8.1 8.0 45.2% Conductivity (pmhos/cm) 263 256 250 253 245 249 256 _ Temperature (C) 25.1 24.2 24.7 24.3 24.8 24.2 24.9 24.2 24.8 24.6 24.9 24.5 25.1 24.3 pH (SU) 7.83 7.55 7.83 7.47 7.71 7.38 7.79 7.34 7.61 7.29 7.72 7.44 7.71 7.59 DO (mg/L) 8.3 7.8 8.2 7.1 8.0 7.0 8.3 6.8 8.2 6.5 8.1 7.1 8.2 7.9 72.6% Conductivity (pmhos/cm) 227 216 215 212 211 214 28M Temperature (OC) 25.1 24.3 24.7 24.5 24.8 24.4 25.0 24.4 24.8 24.3 24.9 24.5 25.1 24.2 PH (SU) 7.82 7.52 7.79 7.47 7.63 7.36 7.76 7.33 7.54 7.28 7.66 7.44 7.66 7.58 DO (mg/L) 8.3 7.8 8.2 7.3 8.0 7.1 8.3 7.7 8.3 6.6 8.2 7.1 8.2 7.9 1000 Conductivity (pmhos/cm) 188 176 178 174 173 176 181 Temperature (OC) 25.1 24.5 24.8 24.3 24.8 24.4 25.1 24.3 24.8 24.4 24.8 24.5 25.1 24.3 pH (SU) 7.81 7.44 7.78 7.45 7.54 7.32 7.74 7.33 7.36 7.24 7.66 7.44 7.66 7.64 0DO (mg/L) 8.4 7.9 8.3 7.4 8.1 7.0 8.4 6.9 8.3 6.5 8.1 7.4 8.0 7.9 1 TConductivity (gmhos/cm) 188 174 177 172 169 171 176 Temperature (C) 25.1 24.5 24.8 2441 24.8 24.2 25.0 24.3 24.7 24.5 24.8 24.3 25.0 24.2

Environmental Testing Solutions, Inc. Page 5 of 6 Environmental Testing Solutions. Inc. Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: 0S-_-(71 Daily Chemistry: l I Day Concentration Parameter CONTROL pH (S.U.) j, 2 , 2_ I DO (mg/L) 0 Conductivity -- 6 (ýImos/cmn) Temperature (°C) 7S.O "'l1

4. 2,-

-'7LI.

2..3 11.3%

DO (mg/L) 32 Conductivity i(pmhos/cm) 308 -31ý0 Temperature(0 C) (Q $.O ",.4 4.1 t".." pH (S.U.) 22.6% DO (mg/L) Conductivity (imhos/cm) Temperature (°C) "?.. C)2 pH (S.U.)

1.

i 45.2% DO (mgf/L) 9, Conductivity (jirnhos/cm) Temperature (0C) is. xt. z'. *, 7... 72.6% DO (mg/L) 0 Conductivity /1 (ýImhos/cm) _ Temperature (°C)

s.I s.3 2A.S 7.1.

2 100% DO (mg/L) Conductivity (ýtrnhos/cm)Me- _______Temperature (QC)

2.

~ 14

2.

V.,f 2 100% Intake DO (mgIL)83 Conductivity 6ýimhos/cm) Temperature (0C) l.I V. I -'.2~- Initial Final I Initial 11 Final I Initial Final Page 56 of 92

Environmental Testing Solutions, Inc. Pag-e 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephlales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: 0<" -10G1 Day 3 4 5 6 _________Analyst 1/4-vt-- Concen-Parameter tration CONTROL pH (S.TJ.) 7,77 1 t DO(m 8 Conductivity (jLrmhos/cm) 3L, Temrperature(°C) -'4. '1.'. lA.q -zQ.' zJ-*, zA.. -'4.. pH(s.U.) "T.eqL- .z T.Ci 11.3% DO6 (m.-. Conductivity (prnho s/cm) A 9 Temperature ('C) -L.. 7.. .4. "4-22.6% DO (mg/L) Conductivity (pnh os/cm) ~-- _______Temperature ('QC) Av PH (S.U.) 1,63 -3ý -7hL v4 ~ 45.2% DO (mg[L) 0380 Conductivity ý (prmhos/cm) _____Temperature (T) ?* -LA*8 22.1 Z4.) PH (S. U.) ( 72.6% DO (mg/L) 0,3S

e. 7-On ConductivityIr

(ýmhos/cm) 9, _____Temperature ('C) 2IS.0 PA - q.c 9l5. IS 100% DO (mg/L) Conductivty ______Temperature ('Q) 2S.1

L4

'4&

z.

4-7AS2e2. jpH (S.U.) /Ij 1/-.533 ý- I --ýJ Ii --

h.

c I }tV+/- q q-.~ 1 - uLu II 11 t-1-U, I I r m 100% Intake DO (mg/L) IConductivity 191 I -- l z. S 8;O t Temperature (T) Initial Final Initial Final Initial Final Initial i al Page 57 of 92

Environmental Testing Solutions, Inc. Page -7 Page of Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst A Date analyzed Calibration: 0.10 mg/L 1.00 mg/L Reference standard number q 7.- "I L-- Iodide reagent: i-t" zs, \\ Acid reagent: c.i4' 2z ,Vote: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) I f-1.ý q a, I-- 0ý.50 ol.,-64 q.2.07'. Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) ~Blank (should be=<0.10 mg/b)

0. 0(o LIS 2 0-105 0 1 SQt.

t to --rTniI C.e.4, ?N tC.LeS 0.00 1q9 011!Te O' k 0Z St3

rvA OT T~J.

CUeAf'. ________<5 C3 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) MIeasured value (MV) % RS = MV /TV x 100 number (mg/L)[ (mg/L) (acceptable range = 90 to 110%) I r rsS q e7_ 0.50 (1. 10 1 q 1-. 2 %7° Reviewed by a Date reviewed 0[

5

-O Page 58 of 92

Environmental Testing Solutions, Inc. -5D.-7 Environmental Testing Solutions. Inc. 5077 Page : Page I of I Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst ,7:I Date analyzed -I-0L Iodide reagent: C Ni'4 3, Acid reagent: "Lr ?,cg Calibration: 0.10 mg/L 1.00 mg/L Reference standard number I I T"Sswga, Note. For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) Md-o 0.50 O.'q .3. 0 C3 Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = ((S - D) /[(S+D)/21} x 100 number (mg/L) (acceptable range +/- 10%) Duplicate W*. D U5o.ou Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number g/L) ~ '.~t~~ Blank (should be =< 0.10 mg/L) t .2 i ~ ~ L3 ~D 0-**tob T\\ A - S N-IN+.-s .qt'VAq UtnuAq"altUf,wo rnloy .,,.~t It 4-0 -0(4A. m~* o". h*. x/'ck c.te~* o co~ cjv-L.oDot9*\\ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV /TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 o.0oo Io0 1 Reviewed by Date reviewed L

3 iom-Page 59 of 92

Environmental Testina Solutions, Inc. 5()- %0.5 Environmental Testin2 Solutions. Inc. 5C.3 Page "} Page of j Total Residual Chlorine (Orion Electrode Method, Orion 97-70) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pt'Ion Meter Analyst lt 15 Date analyzed Iodide reagent: C Acid reagent: tZN *%Z? Calibration: IF _. I 1 2 1 1 7 0.10 mg/L 1.00 mg/L Reference standard number I -*I,'Sqltx, Note: For samples with a residual chlorine of > 1-0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (rag/L) (acceptable range = 90 to 110%) 0.50 0., t4 61 C 2, Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) ~~ ~~ Blank (should be=< <0.10 mg/L) i .y '., ooz.C 7'J ,,-SGVA-k O ,Lc,'No Ur oy, L83 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 0.14-q q.B 0[ Reviewed by Date reviewed Page 60 of 92

C. Pa,,e ____ _ of. Environmental Testing Solutions, In Analyst t Date analyzed -03 Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCOI/L Titrate samples to pH = 4.50 S.U. Time initiated Time completed Titrant normnality and multiplier determination: pH of Normality Deionized Titrant check Begin E water reference standard ml = 4.5 S.U. number number S L\\ItL.J i?* s -2 O.t 1:-9 I-i I nd II Total lil (E) Normality (N) of 1-1SO0 - (5 n-l Na 2CO3 x 0.05)/E = 0.25/E (acceptahle range = 0.018 - 0.022) I I)H Factor or Multiplier = (N x 504)00) 1010) ml sample = IV x 500 -3 12-5 0,o Cy25c wI/. JtwTf 0,-o .o o-o Laboratory control standard." Reference standard True value Sample Alkalinity (MV) % IZS = MV / TV x 1)0) number (TV) volume Begin End Total Multiplier (n1g CaC()VIL) (acceptable range (Ing CaCO:/L) (nIJ) nIl nil taft 90 to I 110%) .100 100 1A3

.5 o
02 q-Duplicate sam le precision:

Sample Alkalinity %RPD = Sample Sample 1U) volume Begin End Total Multiplier (iag CaCO.v/L) ((S - )) /[(S+D)/21) x 100 number (nml) nil ml nil (acceptaable range = t 10%) i" 10 n~ -13Ž>6/6 DUPlicate (B) ~-+3 ý5. D Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (ing CaCOJL) (Ing CaCO3/L) (ml) ndI nIl ml \\ _f i6b -b 3 llA.tp Ko2 rig Sample alkalinity (B) Measured spike value (MV) % R = NIV / SV x 100 (iug CaCO.gL) MV = A - B (acceptable range (,ug CaCOVL) 75 to 125%) 69 5 Oz ('. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (nml) nil ml ml Multip)lier (to" CaCOI/I/) r,- g,,_ as* r 4 ,..-,.,I _ _ _ _ _ __LI_ A'gn, mu.s U) t ,/ .Lo .5.o c.. Reviewed by: Date reviewed: I 14l 01 Page 61 of92

En I P Q-0 vironmental Testing Solutions, Inc. I Analyst i I-Date analyzed O,,.t u'"Y Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO 3iL Titrate samples to pH = 4.50 S.U. Time initiated Time completed ..............--;-- an-d A nutinlier determination: 1 ttrani flu r .. _py f Normality Normality (N) of H2SOA pH Factor or MNultiplier Deiuniz 1 e-T e!nt "i------ n End Total = (5 inl NaCO3 x ).05)/E = (iV x 50000)/ 100 ml sample water reference standard nil -n--- n -- =-- 0.25/E = N x 500 = 4.5 S.U. number number (E) (acceptable ra-ge2 Laboratory control standard. R -eferencestandard True value Saiple Alkalinity (lIV) % RS = MV / TV x I00 number (TV) volume Begin End Total Multiplier (nig CaC()JL) (acceptable range (ing CaCOVdL) (nil) ril nil ml =90 to 110%) ijsq 3 100

9to5, im.Zo.-

'04, YqC~~t)7 Duplicate sam ple precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (nig CaCO:L) {(S - D))/%(S+D)/2]} x l00 number (nil) nil nil nil (aceptatbl+/-e range = t 10%) o*'2-* *tg?*/l_ cO 0.0 C,6.

5.

10. Z-n I Doplicate (B) h 1

4D _____.__,,b' D I t Matrix spike recovery:. Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (ing CaCO.JL) (Ing CaCOVL) (nid) nil m1 nil Sample alkalinity (B) Measured spike value (MV) % I R MV / SV x 100 (ing CaCO.VL) MV = A - B (acceptable range (!ug CaCO:VL) = 75 to 125%) It)n~ten L t'______ -0 01 Sample measutrements." Sample volume Begin End Total Alkalinity Sample number Sample ID (nml) nil nl ml MNIultiplier (1ug CaCOV)'l o - fll. tJCSW 00 t. crl3 c_--3q.L L\\ ( Lk2 CJ,.oo'c*.o-l_ J'A-5O.<, iQ * '-o.s Lqk.b .S 0_ C 0"5--. _Q 23 _0 ___0

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6) -)

11-. ("7o a-1osl-oI.O 'A~A )\\ -:-t '_J cq.et uXJ ossj 07 Reviewed by: Date reviewed: Io O ti-Page 62 of 92

Environmental Testing Solutions, Inc. Pe f_____ Ana!vst Date analyzed (". A -U"] Ti/rant normality and multiplier determinatio Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCOJ/L Titrate samples to pH = 4.50 S.U. Time initiated Time completed PH-J of oeniized Titrant wvater referei-iie-4.5 S.U. numl)er Normality check Begin -standard-. L-__ number End nil11 I Total ]IlI -TE--g* Normality (N) of H-1,SO4 S(5 nil NaC03 x 0.05)/E = 0.25/E -'-aztccletfde--rLang.c -_.01.8 - 0.022) pfl Factor or1 Nfvultiplier =(N x 34000)! 1041 nil samp~le = N x 500 r 1 r I U I S Laboratory control standard: lReference standard True value Sample Alkalinity (MV) O/% RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Ing CaCO.h/L) (acceptahlc range (mag CaCO:L) (nil) llt ml tii 9i) t,, 10%) S 1 100 too c 1'3, 010 I0h-0 I Duplicate sam ple precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (Ing CaCOJL) ((S -D) /[(S+D)/2]r x 100 number -WfA -S(- *J 0 V 3 (nil) nil nml I I (acceptable range t +/- 10%) Duplicate (B) D 4O. z0.0 Matrix spike recovery: Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mag CaCOVL) (rag CaCOv`L) (nil) ml nil nil 1Sal 0-ý 5vi 100 ),.,..3 ...c). q.-+ )0.7_ q(:: Sample alkalinity (B) Measured spike value (MV) ""% R = MV / SV x 10)0) (Og CaCO.VL) MV = A - B (acceptable range (rag CaCOý/L) =75 to 12.50/) (a I c4

.*i Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (II' (nl) l nIl ml Multiplierij (tlg CaCOVL) al0S-2q~ 146~ 1,Y -i0 -q" A 4o 101 alo 0-5 S 1....u 3&. Y, f. .3 0~jl t Reviewed by: Date reviewed: I -i' Page 63 of 92

Page

t Page _

_of 2 Environmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Time initiated Date analyzed iT.. Time completed Titrant normality and multi lier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E - (N x 50000)!50 ml sample number number (E) (acceptable range = 0.018 - 0.022) =N x 1000 ,'A} Ss -NS.l- -,-4 0.0-0 O. O. Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS =MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO3/L) (ml) ml ml nil 90 to 110%) ! s *40 50 A.-

0.

2!

~.

cz O o. Duplicate sam ple precision:. Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3JL) ((S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range = +/- 10%) Duplicate(B) D Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) mnl ml rml Multiplier (mg CaCO/L) Blank (should be 0 mg CaCO3/L) O C. (>0 0.*C, F')D 13jA F KY) i3l/) 9-4. 4I 05__6___ 6LL~L LI~ os-u-oleA o.o qI 4.:. ,o_ 6S____ .3 C7cZoI O'SS(S-f J 0

12. Z Note: If >l5ml of titrant is used, sample must be diluted.

Page 64 of 92 Reviewed by: Date reviewed L -i'i

Page Page of Environmental Testing Solutions, Inc., Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg.CaCO3/L Analyst Time initiated Date analyzed Time completed Titrant normality and multiplier determination. .itrant, Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference- .*tindard. -ml----... mi m......m...... ml = 0.2/E = (N x 50000)150 ml sample number number (E)

acceptablerange_-0.018 - 0.022)

= N x 1000 Laboratory control standard:' Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3JL) (acceptable range (mg CaCO/L) (ml) ml ml ml = 90 to 110%) IiYS5q3* 40 50 1 q.2 2, 2o.2 *00.G. Duplicate sam ple precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range = +/- 10%) Duplicate (B) Dl. 3.1 Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL) Blank ~- Or z -(10lb-3-Qe-5)- Po-cn uQ2~ o\\ si/ So) 3 .3b Z 3_-

,0.2-C1 k*.A 2."__

3 1 3 co -1,oL-4.,5 1

3. *.

-1i ___I OZ0 IG _S2.0Z-- 31 1 3L4, P 5 -i [Cr7 Os l,1 05 0"1 CGo 1%. 6 Z jC; 2Lr__jII I[ Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Page 65 of 92 Date reviewed Ok - I

Sequoyah Nuclear Plant Biomonitoring May 30 - June 6, 2007 Appendix D Reference Toxicant Test and Control Chart Page 66 of 92

Environmental Testing Solutions, Inc. Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. I.: 1.0k 0.8 SIiIII I I I Ii USEPA Control Limits (+/- 2 Standard Deviations) II I I I III 0.6 V 0.4 1.2 N-1.0 F 0.8 F !I I I I I I I I II USEPA Warning and Control Limits ( 75th and 9 0 th Percentile CVs) I III I I I I I I 0.6 0.4 1.2 I I I I I I I I Laboratory Warning and Control Limits (10th and 25th I I I Percentile CVs) 1.0 V 0.8 1 0.6 V ....... I....... I I 0.4 'Z1 'ri, l Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25) Warning Limits (mean IC2 5 +/- SA.0 or SA.75) Control Limits (mean IC 25 +/- S A.25, SA.90' or 2 Standard Deviations) Page 67 of 92

Environmental Testing Solutions, Inc. Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. State and USEPA Laboratory Laboratory S Control Limits SA.D Warning Limits Sý25 Control Linits CT-2S CT+2S CT-S,1 5 CT+SAIo CT-S,,, CT +SA. 2 5 Test number Test date 7-dayICzs CT (gi. KCG) (g/L KG!) USEPA SxTs Warning Limits Sý90 CT - S,,, CT + S,, USEPA Control Limits CT - S,9, CT + S,.o CV 1 2 3 4 5 6 7 8 9 10 II 01-09-07 0.67 01-23-07 0.63 02-06-07 0.72 02-13-07 0.69 02-13-07 0.66 02-20-07 0.59 03-06-07 0.71 04-03-07 0.53 04-03-07 0.79 05-08-07 0.64 05-30-07 0.62 0.65 0.67 0.67 0.67 0.66 0.67 0.65 0.66 0.66 0.66 0.03 0.59 0.70 0.08 0.57 0.72 0.14 0.51 0.78 0.25 0.40 0.05 0.58 0.76 0.08 0.59 0.75 0.14 0.53 0.81 0.25 0.42 0.04 0.60 0.75 0.08 0.59 0.76 0.14 0.53 0.82 0.26 0.42 0.03 0.60 0.74 0.08 0.59 0.75 0.14 0.53 0.81 0.25 0.42 0.04 0.57 0.75 0.08 0.58 0.74 0.14 0.52 0.80 0.25 0.41 0.05 0.57 0.76 0.08 0.59 0.75 0.14 0.53 0.81 0.25 0.41 0.06 0.52 0.78 0.08 0.57 0.73 0.14 0.51 0.78 0.25 0.40 0.08 0.51 0.82 0.08 0.59 0.74 0.14 0.53 0.80 0.25 0,41 0.07 0.52 0.81 0.08 0.58 0.74 0.14 0.52 0.80 0.25 0.41 0.07 0.52 0.80 0.08 0.58 0.74 0.14 0.52 0.80 0.25 0.41 0.89 0.29 0.36 0.92 0.30 0.37 0.93 0.30 0.37 0.93 0.30 0.37 0.91 0.30 0.36 0.92 0.30 0.37 0.90 0.29 0.36 0.92 0.30 . 0.37 0.91 0.30 0.36 0.91 0.30 0.36 0.94 0.04 0.97 0.07 0.98 0.06 0.97 0.05 0.95 0.07 0.97 0.07 0.94 0.10 0.96 0.12 0.96 0.11 0.96 0.11 Note. 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassiumn chloride that would cause a 25% reduction in Piniephales growth for the test population. CT = Central tendency (ussean IC2,). S = Standard deviation of the ICý, values. Laboratory Control aid Warning Limits Laboratory control and warning limits were established using tie standard deviation of the IC2, values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits reconmmended by USEPA for the test method and endpoint. S,10 = Standard deviation corresponding to the 10h percentile CV. (S*,o = 0,12) S,25 = Standard deviation corresponding to the 25'h percentile CV. (S.2, = 0.21) USEPA Control and Warning Limnits S.7s = Standard deviation corresponding to the 750 percentile CV. ( SMs = 0.38) S.90 = Standard deviation corresponding to the 9 00' percentile CV. (SA90 = 0.45) CV = Coefficient of variatons of the IC2s values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833 -R-00-003 US Envirosnnental Protection Agency, Cincinnati, OH. Organimin obtainedfrem AquaoxtoIc. Je tx~O0 atox05-30-07

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimephales promelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Organism Source: Aquatox, Inc. Test number Test date Control Survival Control Mean Growth CT CV CT MSD PMSD CT for Control Growth (%) (mg/larvae) (mg/larvae) for Control Growth CV (%) (%) for PMSD (%) 1 2 3 4 5 6 7 8 9 10 11 01-09-07 01-23-07 02-06-07 02-13-07 02-13-07 02-20-07 03-06-07 04-03-07 04-03 -07 05-08-07 05-30-07 100 100 100 100 100 100 100 100 100 100 100 0.724 0.665 0.673 0.567 0.598 0:53 1 0.734 0.724 0.763 0.883 0.790 0.695 0.687 0.657 0.645 0.626 0.642 0.652 0.664 0.686 0.695 9.1 2.9 12.3 4.3 10.6 6.3 15.6 14.8 3.7 6.1 12.2 6.0 8.1 7.1 7.8 7.6 8.7 9.5 8.8 8.6 8.9 0.10 0.10 0.10 0.06 0.08 0.12 0.12 0.11 0.12 0.09 0.11 13.4 15.3 15.0 10.4 12.6 22.8 15.8 15.8 15.9 10.5 13.6 14.4 14.6 13.5 13.4 14.9 15.0 15.1 15.2 14.8 14.7 Note: CV = Coefficient of variation for control growth. Lower CV bound determined by USEPA (10 th percentile) = 35%. Upper CV bound determined by USEPA (90"L percentile) = 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of testprecision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (10' percentile) = 12%. Upper PMSD bound determined by USEPA (90th percentile) = 30%. CT = Central Tendancy (mean Control Growth, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003..,S Environmental Protection Agency, Cincinnati, OH. USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox, Inc. Page 69 of 92 atox05-30-07

Environmental Testing Solutions, Inc. Pimephales promelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests Organism Source: Aquatox, Inc. 1.25 E IIII I I I I I II 0 .0 0 0.. 1.00 H 0.75 0.50 0.25 30 20 10 0 30 20 10 0 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I I I I I I I I

I

[I iI I Kentucky Acceptance Limit (< 30.0%) !I I I I I I I SII I I I I iI USEPA Upper PMSD Bound (9 0 1h percentile < 30.0%) / 01 1z 6'z' 0ý0-vjl Test date 0 Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations) Pao*p 70,f o

Environmental Testing Solutions, Inc. Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: '2' Dilution preparation information: Comments: KCI CHM number: C.1' 5 Stock preparation: 50 g KCI/L: Dissolve 50 g KCI in 1-L Deionized water Dilution prep (mgfL) 450 600 750 900 1050 Stock volume (mL) 9 12 15 18 21. Diluent volume (mL) 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: "-2X-op..k* Randomizing template: l_ v .e Date and times organisms 05. -zq -] A00 Incubator number and were born between: shelf location: Organism source: qm-A 6 ArTA Pe 05-..-cl Artemia lot number: 66o02Dq L) Transfer bowl information: pH = SU Temperature = 'C Total drying time: "N-146tim

1.

"5 Date / Time in: 0*.-a0"1 itac> Average transfer volume:. Date / Time out: oto-o~-1 1-% Oven temperature: ,.C Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time renewal, or batch used time termination time ooSZoo

q24 1

1 _Z os-z-i A 3 0o¢6-crn 0qoo 6 SOO I M50 os-zS- - 4 0 6- 0-61-o -7 0 11 0 0 L S o o 12 4 1, o ) - 3 %- c-7 A' 6 -OV 1 o 3-fi A 06-6-01 ~ ~ 'kj' 060 L R S3-Control information: Acceptance criteria Summary of test endpoints: % Mortality:

5. <20%

7-day LC5 0 Average weight per initial larvae:

0. ii 0 i*/<1/24t-

!,>J NOEC 0 '4&C Average weight per surviving larvae: O.

0

Ž0.25 mg/larvae ALOEC s4 O ChV <',so IC2s IJ *L%, (C Page 71 of 92

Environmental Testing Solutions, Inc. Page 2 of 5 Species: Pimephlales promelas PpKCICR Test'Number: ___q Survival and Growth Data Day Control 450 in KCI/L 600 mg KCI/L A B C D E F G H I J K L 10/0 1 /0 10 1 16 o0 C) 10 10 0 /0 1C0 /0 1 /0 /0 0 /0 /0 to /0 /0 to to 10 2 2 /0 to to I() I /0 tO1 to 10 1O 1O b 1 30 10 (0 10 /0 10 1(3 (0 10 tO /0 'l O /0 I 0 10 00 10 /0 ')/ 5 10 to to to /o(0 0/0 /10 10 tO

65.

10 t0 1 /0 /0 10 /0 to 1O tO Io (D /0 IO /0 I/ /0 q, 1 /0 q 7(0 li Io /0 tO /0 /0 /0 'q, 10 /0 A = Pan weight (mg) Tray color code:: .,e'C -" c' Analyst: ,*k q "Z g 5H-13 13VO-fO141 <*T

.lq*71*g o.

B = Pan + Larvae weight (mg) Analyst: XA-9. a'a.% i5 '&.W( ]a.,cj At. 0 , iq.5 *Ao.3% AoM ?J.-% ao.A5 M,.a C = Larvae weight (mg) =A-B Weight per initial number of larvae (mg) = C / Initial number of larvae 4, 1~ I', 0' Nb '9 '.9 I'. '7' '.0 'a O0 0 4, 0' 4 V C) 'IV 0' Average Percent weight per reduction initial from control .1 number of (%/) larvae (mg) C), "05 -z5.'7" 0.(. t 9.V Comment codes: c = clear, d= dead, fg = fungus, k = killed, m =.missing, sk =sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed:&_ Comments: Page 72 of 92

Environmental Testing Solutions, Inc. Paze 3 of 5 Species: Pimnephales promelas PpKCICR Test Number: i-L_ Survival and Growth Data Day 750 mg KCI/L 900 mg KCI/L 1050 mý KCI/L M N 0 P Q R S T U V W X 0 Lo 10 10 I0 10 10 ID) 10 1C 10 IC t0

z.

bA WL tA 1At 2 A. 3 Ok C, -,-A 4 1 to -*,4 5 414 4

2.

,3 \\ Tray color code:: ~ ~ -~ ~ 33 jI ~1V 31 ~ ~ I 5 .i9 B = Pan + Larvae weight (rag)r Analyst: 11854 1. B.S 1 17.10 C l.Bto il.031 L1.14 14.b'J 1q.0% 13.1(0 s.98, i*lo C = Larvae weight (mg) =A-B 1 q4.6'1 Z'.J'L *SVo -L. z.4 \\ o.'A o.W1 0 .- 1 o.'- Weight per initial number-,\\ of larvae (mg) A ON M) 0 0 C Initial number oflarvae 4 -V 0' o" 0' 1 o t" 0 0' o 0. Average Percent weight per reduction initial from control 0 0 o.24 5 6q.lT7 5 7. number of (N) larvae (mag) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:I--- Comments: Page 73 of 92

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Organism Source: Aquatox, Inc. Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR# 124 Test dates: May 30 - June 06, 2007 Reveiwed by: Con.centrtioro.e (mgIL Replicate Initial number of Final number of A Pan weight (mg) - Pn + Loarvae Larvae weight (rag) Weight / Surivg HMo. weight/ Coefficient of variaon Weight I Initial number Mean surooval Mean weight/Initial C cet of Percent reduction from KCI) I.r-oe I.t-ce weight (rag) = A-number of larvae.(tagl Slorvicig.n.tberot (vt. oatoto.el of 1-ro.. tog) fl) nomber oflt r2. variation (.) control (.) lorvoe (mog) ... 0,* k-) .t (%) (mg) A 10 10 14.72 22.95 8.23 0.823 0.823 Control B_10 10 15.05 23.93 .88 8 0.790 12.2 0.888 100.0 0.790 12.2 Not applicable C 10 10 14.73 22.62 7.89 0.789 0.789 D 10 10 13.60 20.19 6.59 0.659 0.659 E t0 10 13.91 20.46 6.55 0.655 0.655 450 F to to 14.40 21.03 6.63 0.663 0.605 10.5 0.663 100.0 0.605 10.5 23.5 G 10 10 14.11 19.52 5.41 0.541 0.541 H 10 10 14.79 20.38 5.59 0.559 0.559 1 10 9 14.17 20.11 5.94 0.660 0.594 600 J 10 10 14.47 21.25 6.78 0.678 0.676 6.5 0.678 95.0 0.641 5.8 18.8 K 10 10 13.92 20.23 6.31 0.631 0.631 L to 9 14.63 21.25 6.62 0.736 0.662 1 M 10 7 13.85 18.42 4.57 0.653 0.457 N 10 6 14.55 18.54 3.99 0.665 0.399 750 0.643 6.2 62.5 0.404 20.0 48.8 0 10 7 13.13 17.82 4.69 0.670 0.469 P 10 5 14.78 17.70 2.92 0.584 0.292 Q 10 5 14.32 17.68 3.36 0.672 0.336 900 R t0 4 !3.88 16.34 2.46 0.615 0.636 13.3 0.246 37.5 0.245 40.7 69.0 S 10 4 14.23 17.14 2.91 0.728 0.291 T 10 2 13.81 14.87 1.06 0.530 0.106 U 10 1 13.84 14.58 0.74 0.740 0.074 V lo 2 13.29 13.96 0.67 0.335 0.501 34.3 0.067 10500.03437. 0.353785 W 10 3 14.51 15.98 1.47 0.490 0.147 X 1o0 1 14.42 14.86 0.44 0.440 0.044 Dunnett's MSD value: PMSD: 0,1078 13.6 MSD - Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound detertisined by USEPA (10th percentile) = 12%. Upper PMSD bound detenssined by USEPA (90th percentile) = 30%. Lower and upper PMSD bounds were deternined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200 1a; USEPA, 200tb). USEPA. 200(a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-ternm Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Etsvirotnental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatox. Inc. atox05-30-07

Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/30/2007 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 6/6/2007 Lab ID: ETS-Envir, Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pirnephales promelas Comnments: Conc-mg/L 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.9000 1.0000 1.0000 0.9000 750 0.7000 0.6000 0.7000 0.5000 900 0.5000 0.4000 0.4000 0.2000 1050 0.1000 0.2000 0.3000 0.1000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9500 0.9500 1.3305 1.2490 1.4120 7.072 4 14.00 10.00 2 40

750 0.6250 0.6250 0.9134 0.7854 0.9912 10.805 4

10.00 10.00 15 40

900 0.3750 0.3750 0.6546 0.4636 0.7854 20.756 4

10.00 10.00 25 40

1050 0.1750 0.1750 0.4217 0.3218 0.5796 29.582 4

10.00 10.00 33 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95849895 0.884 -0.3201935 0.12279251 Equality of variance cannot be confirued Hypothesis Test (H-tail, 0.05) NOEC LOEC ChV TU Steers Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 10.2420223 1.34262248 7.61048221 12.8735624 0 1.15610913 7.81472778 0.76 2.92182665 0.09763697 3 Intercept -24.925414 3.91623483 -32.601234 -17.249593 TSCR Point Probits mg/L 95% Fiducial Limnits ECOl 2.674 495.095634 411.397448 554.477589 EC05 3.355 577.06951 503.787688 628.611518 EC1O 3.718 626.180937 560.512776 672.980097 EC15 3.964 661.656281 601.771683 705.349552 EC20 4.158 691.278908 636.167841 732.816148 EC25 4.326 717.747214 666.658351 757.890818 EC40 4.747 789.024716 745.944742 829.572331 EC50 5,000 835.269552 793.774299 880.709652 EC60 5.253 884.224814 840.795763 939.308298 EC75 5.674 972.034731 917.880584 1053.81268 EC80 5.842 1009.25288 948.678054 1105.02979 EC85 6.036 1054.43757 985.084894 1168.82582 EC90 6.282 1114.17509 1031.98408 1255.44824 EC95 6.645 1208.99682 1104.35347 1397.40266 EC99 7.326 1409.17275 1251.51604 1711.89702 Organisms obtained fiom Aquatox, Inc. atox05-30-07

Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/30/2007 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 6/6/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Conc-mg/L 1 2 3 4 D-Control 0.8230 0.8880 0.7890 0.6590 450 0.6550 0.6630 0.5410 0.5590 600 0.5940 0.6780 0.6310 0.6620 750 0.4570 0.3990 0.4690 0.2920 900 0.3360 0.2460 0.2910 0.1060 1050 0.0740 0.0670 0.1470 0.0440 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7898 1.0000 0.7898 0.6590 0.8880 12.201 4 0.7898 1.0000

450 0.6045 0.7654 0,6045 0.5410 0.6630 10.495 4

3.745 2.180 0.1078 0.6229 0.7887

600 0.6413 0.8120 0.6413 0.5940 0.6780 5.778 4

3.002 2.180 0.1078 0.6229 0.7887 750 0.4043 0.5119 0.4043 0.2920 0.4690 19.996 4 0.4043 0.5119 900 0.2448 0.3099 0.2448 0.1060 0.3360 40.666 4 0.2448 0.3099 1050 0.0830 0.1051 0.0830 0.0440 0.1470 53.674 4 0.0830 0.1051 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96893233 0.805 -0.5679795 0.21604887 Bartlett's Test indicates equal variances (p 0.34) 2.15916371 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob. df Dunnett'sTest <450 450 0.10784147 0.13655141 0.03848025 0.00489428 0.01059279 2,9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05* 106.48 33.37 67.60 277.86 1.8913 IC10* 212.97 73.76 135.19 555.71 2.5016 IC15* 319.45 103.29 202.79 794.72 1.4895 IC20* 425.93 117.18 270.38 776.58 0.2465 IC25 620.97 78.19 284.85 705.59 -1.5867 IC40 702.25 23.97 647.25 787.49 0.3507 IC50 758.82 29.27 689.40 846.43 0.0840

indicates IC estimate less than the lowest concentration Organisms obtained from Aquatox, Inc.

atox05-30-07

Environmental Testing Solutions, Inc. Page 4 of 5 mnalTstn oltosIc.Pg 4o Species: Pimeph ales promelas PpKC1CR Test Number: 114 Dailv Chemistr: Day Analyst -ic Concentration Parameter CONTROL pH (S.U.)q8 '4. (0, -.4-5 --_i - 3 9. - DO (mg/L) '162 Conductivity ([imhos/cm) 3 Alkalinity (mg CaCO 3/L) G 0to Hardness (mg CaCO 3/L) C( Temperature t! I (0c) -t____, -__.2_. pH (S.U.)

.OZ j,5*

d Ls ._o DO (mg/L) 450 mg KCI/L Conductivity (ymnhos/cm) 10 Temperature _L _____(00) 1A' -q* L.O -tq. S~ z7.t.2. pH (S.U.) 5 5- ?.cL $11 I DO (mg/L) z -.+3o 600 mg KCI/L Conductivity 0 (1imhos/cm) Temperature (00) pH (S.U.) IT. T-S* .-Yq '.i2. 2w, DO (mg/L) 1 o 750 mg KCI/L Conductivity (pnmhos/cm) '-L 0 )o Temperature 7 .(0 Z. Lq. (00) DO (mg/L) 0 [ .3 -17.4 900 mg KCI/L Conductivity (pmhos/cm) }e 0 LO )C5]8C Temperature 5 1"1( (0c) pH (S.U.) b~v3 I qeq 'T5 53/4 DO (mg/L) 1050 mg KCfL Conductivity (iimhos/cm) Temperature (0C) zS10 zt q& zVZq.,3 Q (000 Initial Final Initial PFinal Initial WFinal STOCK Conductivity I (!imhos/cm) Page 77 of 92

Environmental Testing Solutions, Inc.Et Page 5 of 5 .2 Species: Pimeph ales prom elas PpKCICR Test Number: rLA ____Day -3 4 6 Concentration Parameter CONTROL pH(S.U.) q, 14 "T 6q "-1._*1 -41 DO (mg'L) J7o Conductivity (pimhos/cm) A Alkalinity (mg CaCO 3/L) Hardness (mg CaCO 3/L) Temperature H (S.U.c) qq. 1j DO (mg[1L) V 450 mg KCI/L Conductivity U (pmhos/cm) Temperature (°c) 1S. 0 pH (S.U.) -4,+5 1-.1 q-.LA-__ DO (mg/L) I 600 mg KCIL Conductivity (_imhos/cm) 1aqo 13-20 I-*YO 0 Temperature 2'. 7qs lq.' N.1 7Lq A (________ iz v*.a; I o I ______._*q qq qf

q.

750 mg KCI/L DO(mg/L) b (0.01 "1-9 Conductivity (mhos/cm) I6 Temperature -Zq., .1A zq.2 L

q.

(OC) V-1zA' pH (S.U.) .c*c t-A-{ 1 _-T __ DO (mg/L) t.6 900 mg KCL/L Conductivity (p mhos/cm) )64o 1O 1-i-,fO. Temperature o).. "4. a -q Z4.o zj L'4.s 25.0 2V-t (OC) T___4 ____S VS___ pH (S.U.) '1I ) 1% -1. 1 ~1AC I 'IAO 71-I 5 -V6-A f? 1 ~~- A- _4~ I DO (mg/L) 1050 mg KCVL Conductivity (fimh s/cm) Temperature (0C) e, (0

q

-74 ýD 4 q.q <.t e =

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Qo7 0 0 5S Initial II......... i na I Initial Final Initial Final Initial Final I I _I "I. S rOCK Conductivity (4mhos/cm) ~ I - I Page 78 of 92

Environmental iesting SolUtions, Inc. Sodium Ceriodaphnia dubia Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 1.10 1.08 1.06 1.04 1.02 2.5 I I I I I I I I I ID e I I I I USEPA Control Limits (+-2 Standard Deviations) I I I I I I I I I I I I I I I I I I Q z,)t' J 2.0 1.5 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 SI I I I I I I i I i I I I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs) SI 1 I I I I I I I I I I I I I I I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC25) Warning Limits (mean IC 25 +/- SA.10 or SA.7S) Control Limits (mean IC 25 + SA.25, SA.90, or 2 Standard Deviations) Page 79 of 92

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Test nunber Test date 7-day IC25 CT (g/L NaCI) (g/L NaCI) State and USEPA S Control Linits SA.10 CT-2S CT+2S Laboratory Warning Linits CT - Sk1o CT + SA_10 Laboratory SA.2s Control Linits CT - SAý2 5 CT + SA.2S USEPA SA.*7 Warning Linits CT - SA15 CT + S175 USEPA SAAo Control Linits CT - S, 90 CT + Sx90 CV 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 11-01-05 12-06-05 01-10-06 02-07-06 02-07-06 03-07-06 04-04-06 05-02-06 06-06-06 07-11-06 08-08-06 09-12-06 10-03-06 11-07-06 12-12-06 01-09-07 02-06-07 03-06-07 04-03-07 05-08-07 1.08 1.04 1.08 1.07 1.06 1.05 1.06 1.08 1.06 1.09 1.07 1.06 1.06 1.06 1.09 1.07 1.09 1.09 1.10 1.10 1.06 0.03 1.00 1.11 0.08 0.97 1.06 0.02 1.02 1.11 0.09 0.98 1.07 0.02 1.03 1.10 0.09 0.98 1.06 0.02 1.03 1.10 0.09 0.98 1.06 0.02 1.03 1.09 0.08 0.98 1.06 0.01 1.03 1,09 0.08 0.98 1.06 0.01 1.04 1.09 0.09 0.98 1.06 0.01 1.04 1.09 0.09 0.98 1.06 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.06 0.01 1.04 1.09 0.09 0.98 1.06 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.02 1.04 1.10 0.09 0.98 1.07 0.02 1.04 1.10 0.09 0.99 1.07 0,02 1.04 1.11 0.09 0.99 1.14 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.15 1.16 1.16 1.16 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.89 0.89 0.89 0.89 0.89 0.89 1.24 0.48 0.58 1.24 0.48 0.58 1.25 0.48 0.59 1.25 0.48 0.59 1.24 0.48 0.58 1.24 0.48 0.58 1.24 0.48 0.58 1.24 0.48 0.58 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.53 1.54 1.54 1.54 1.54 1.54 1.54 1,54 1.54 1.55 1.54 1.54 1.54 1.55 1.55 1.55 1.55 1.55 1.56 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.40 0.40 0.40 0.40 0,40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 040 0.41 0.41 0.41 0.41 0.41 0.41 1,71 0.02 1.72 0.02 1.73 0.02 1.72 0.02 1.72 0.01 1.72 0.01 1,72 0.01 1.72 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1,73 0.01 1,73 0.01 1.73 0.01 1,73 0.01 1,73 0.01 1,73 0.01 1.74 0.01 1.74 0.02 Note: 7-d IC 25 = 7-day 25% inhibition concentration. An estimation ofthe concentration ofsodiuns chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the IC25 values. Laboratory Control and Warning Linits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. SA.10 = Standard deviation corresponding to the 10 h percentile CV. (SAs0 = 0.08) SA.2S = Standard deviation corresponding to the 25'h percentile CV. (SA2s = 0.17) USEPA Control and Warning Linits SAKS = Standard deviation corresponding to the 75h percentile CV. (SA7S = 0.45) SA.9o = Standard deviation corresponding to the 900' percentile CV. (SAs0 = 0.62) CV ý Coefficient of variation of the IC 2, values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 0508107

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test number Control Control Mean Test date Survival Reproduction (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 11-01-05 12-06-05 01-10-06 02-07-06 02-07-06 03-07-06 04-04-06 05-02-06 06-06-06 07-11-06 08-08-06 09-12-06 10-03-06 11-07-06 12-12-06 01-09-07 02-06-07 03-06-07 04-03-07 05-08-07 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 28.4 32.6 29.2 30.7 29.9 28.8 27.0 28.6 30.3 29.0 28.6 30.9 32.3 31.0 28.0 27.6 29.9 30.1 33.1 32.0 CT for Control Mean Reproduction (offspring/female) 30.5 30.1 30.2 30.2 29.9 29.5 29.4 29.5 29.5 29.4 29.5 29.7 29.8 29.7 29.6 29.6 29.6 29.8 29.9 CV CT for Control (%) Reproduction CV (%) MSD PMSD 10.3 6.3 4.8 6.0 6.8 5.9 5.2 8.6 5.2 5.4 8.9 5.4 4.6 6.3 6.7 4.9 7.6 6.0 4.4 8.5 8.3 7.1 6.8 6.8 6.7 6.5 6.7 6.6 6.4 6.7 6.6 6.4 6.4 6.4 6.3 6.4 6.4 6.3 6.4 3.8 2.3 2.3 2.4 2.2 2.6 2.1 2.7 3.0 2.5 3.7 3.3 3.1 2.4 2.2 2.4 2.9 2.6 2.2 2.7 (%) for PMSD (%) CT 13.2 7.1 7.8 7.9 7.5 8.9 7.6 9.3 9.8 8.6 12.8 10.6 9.5 7.8 8.0 8.6 9.8 8.7 6.6 8.4 10.2 9.4 9.0 8.7 8.7 8.6 8.7 8.8 8.8 9.1 9.3 9.3 9.2 9.1 9.1 9.1 9.1 9.0 8.9 Note: CV= Coefficient of variation for control reproduction. Lower CV bound determined by USEPA (10 th percentile) = 8.9%. Upper CV bound determined by USEPA (9 0 t percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (1 0 th percentile) = 13%. Upper PMSD bound determined by USEPA (9 0U1 percentile) = 47%. CT Central Tendancy (Mean Control Reproduction, CV, or PMSD) USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. USEPA. 200 la, 200 lb. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH. 05-08-07 Page 81 of 92

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 .0 0 35 30 25 20 15 40 30 20 10 50 40 30 20 10 I I I I I I I I I I I I I[ Minimum Acceptance Criteria (> 15.0 offspring per surviving female) I I I I I I I I i I I I I I I I I North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%) f... ... i........ I....... I I II I I I I I i I I I USEPA Upper PMSD Bound (9 0 th percentile < 47.0%) Ul C '1'0 6"0 6-o 6%, %, 0 JI, NV Vj fj Q;Ycý$Jv NO zip NV vs'v V31V Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Reproduction, CV, or PMSD)

Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations) Page 82 nf92

Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 6_ Dilution preparation information: Comments: NaCI CHM number: 0.4K 1"2-O Stock preparation: 100 g NaCI/1 (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (m/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism information: Test information: Organism age: < 24hours old Randomizing template: oa-______ Date and times organisms o5-u-01 mo'" -M "ieO Incubator number and were born between: shelf location: Organism source: O5-0\\--7A P ,.*,i, 'S,,., t'I, 2.0 YCT batch: o.<A-0"1 Transfer bowl information: pH==1,*1 SU Temperature --Z4.LCC Selenastrum batch: O5-0*-U"1 Daily renewal information: Day Date Test initiation, MHS water Analyst renewal, or batch used termination time 0 Vio-Cs-o\\-(33 O S os-cp-a'1 11 11 Os-ok-o' 6 2 2 -c)-aool till 05-06 7\\ 3 cis_-it-___ _ 115 _s_- 0"1-6 4 O sg -Q - 0' 1 110 9 r'S - O q - C"1 1) 5 as1 1I110 o5 e-6 OS-1q4-O I I 13 O¶-CAi P"11) 4)\\A 7 os ls-cri Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: 011. < 20% 7-day LC50 0> Iqoc % Adults having 3d Broods: /007,. 80% NOEC OCXO % Mortality: 0 20% LOEC 12.00 Mean Offspring/Female: ,370 2! 15.0 offspring/female ChV CA's.4 % CV:

g. S?,

< 40.0 % IC25 101___ JO Page 83 of 92

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR#: (0 CONTROL Survival and Reproduction Data Replicate number Day' 1 2 3 4 5 6 7 8 9 10 1 Young produced (_ ) (_ Adult mortality U 2 Young produced C_ _Z___C Adult mortality \\-- 3 Young produced] C) LcJcIATC) C)TcA0 Adult mortality \\- " kk-.. L_- Z1'._ Young produced 1LkI'1 iI'] I' iu I[ iz TAul mortality L 1 I I f 7 Young produced C>3 "A-1 .I i Total young produced 3" ,2\\ 3 Tb 7-Final Adult Mortality k-A...,__.__ X for 3rd Broods J )C Ž Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: j Mean Offspring/Female: 32..0 600 m NaCIIL Survival and Reproduction Data Replicate number Day_1 213 4 5 6 7 8 9 10 1 Young produced C) 0 C) cQ c) )C) 0 C) [ Adult mortality 1 X- \\ \\* k..._ 2 Young produced [3j 0 )- Adult mortality I.._ Q-3 Young produced j Adult mortality 1_ oVt4o Young produced 4 1 LIA1! Adult mortality L.. k._ k._ '5 Young produced I' -L IL t~ I k s 1 7 I Young produced CS 1 1 1' (.. Iq %A is I*- I S Total young produced ,3 1 3i a s 1% 3"' L 5-. 32-7-1 Final Adult Mortality j k j U. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: Mean Offspring/Female: . o I% Reduction from Control: I .071. Page 84 of 92

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR#: !t6 800 m2 NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced ] 0 0*C Cj C) . 0( Adult mortality k--- -- \\- t_ k.. 2 [Young produced C)l~ C) C) 1c) C) L~ Adult mortality _['- _ [ \\- LJ 3 Young produced [0 oT Adult mortality \\__ [I,- '-I'---[ .- ] LH*., *_ I O [ O 4 Young produced kA L..._ ]L.1 I IAdult mortalityI 5 Young prod uced 1 1 L3 T2_ I IL .l I ~ N z Adult mortality \\ý...J t.- L_ k. 6 Young produced C)_ 01 CQ 01 0T© 1 0 o c. Adult mortality ] \\_..j .[ j [ L,. 7__ Young produced

t.

t 'q Lq L, [ I Total young produced ,.4 3,4 3N Final Adult Mortality C L... Note: Adult mortality (L live, D dead) Concentration: % Mortality: 07. Mean Offspring/Female: ,31.9 % Reduction from Control:

0. -67..

1000 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced ) C) 0_ O* 0 ) 0 Adult mortality I_ 2 Young produced 1o--i l-C101 0l Adult mortality jU J _.- Adult mortality I Y-- 4 Young produced ' AL t.* 7 L AI 4 [ 4 1 4 Adult mortality [.... LI SAdult mortality 9 j LO jA Z{_ l,__ 6 Young produced 1CJ_1 ) C> F _ClO)L i Adult mortality I__ 7 Young producedI U, I L(,Q Lt 7T i l* '1. Total young produced ?-'A 30 2S 2f 31 J Z 2.. 6 k 30 26( Final Adult Mortality ..- I Note: Adult mortality (L = live, D = dead) Con centration: % Mortality: Mean Offspring/Female: 2.. I % Reduction from Control: I i %V7.o Page 85 of 92

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: (A 1200 m~ NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1I Young produced Cý 0 { C 0 0 C Adult mortality I._.. __. 2 Young produced c_ (D101 ol ]I 0 C) (D Ad ltmo tliy.- I \\-.- 1_ 3 Young produced c ) C ) C [Adult mortality k I -K -[~ \\-- 7 Young produced k 4 Young produced 10 1 L~rT, 0Aqq~ ~ Adult mortality t__ - 1' IJ- '1 -J -I" 6 Young produced ]C)_ I_ p_ c_ __I ITJI0 __{Adult mortality Ij_ L-4-'- L ~I~-T->- 6 Young produced 13 -51~c ~ Total young produced Final Adult Mortality j L...

k.

Note: Adult mortality (L = live, D = dead) 1400 me NaCI/L Concentration: % Mortality: = 6-7. Mean Offspring/Female: I l, I % Reduction from Control: I I°7. Survival and Reproduction Data Replicate number Day I 2 3 4 5 6 7 8 9 10 1 Young produced '0

0)

Q 0 0* ___utmotaiy_ _I_ I Adult mortality

t. L L

2 [ Young produced (_) C [*JQ] (C 02) __ I Adult mortality L_ j _LjU ._.J [ I_'" I -- 3 Young produced g c "oC)o"- _jAdult mortality \\-_-~--~-~- L L U L I 4 Young produced LA{ 1'4 1 ~T 1 1 TIIF' 1~ Z) 13 __IAdult mortality J_~ I'J 1' -J-5 Young produced 1~V -3_____ 6] Young produced fjI c 0 1 0 1 C) [ Q I~ Q 801 Adult mortality I I (- I 7] Young-produced ýJ 7 0 3 -z 3 Total young produced f 0 9 Final Adult Mortalit k._ k.. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: lc)% Mean Offspring/Female: 1.q I % Reduction from Control: 1.. "17 I Page 86 of 92

Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control Day Re licate number Dy 1 2 3 4 5 6 1 7 8 9 0 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 444 4 5 5 5 4 4 5 44 5 13 11 11 13 10 14 12 11 11 12 118 6 0 0 0 0 0 0 0 0 0 0 0 7 1 14 16 18 15 17 18 15 14 14 158 Total 34 29 31 35 30 136 35 30 29 31 320 600 mg NaCI/L Day Re licate number Total a1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 6 6 4 4 5 5 4 47 5 13 12 14 13 11 13 13 11 13 10 123 6 0 0 0 0 0 0 0 0 0 0 0 7 14 15 17 16 14 15 15 15 14 15 150 Total 31 31 36 35 31 32 32 31 32 29 320 800 mg NaCI/L Day___ Re licate number Total Day 2 13 JA 5 6 7 8 9 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 4 5 4 5 4 5 5 46 5 13 13 12 14 1 1 11 13 13 14 12 126 6 0 0 0 0 0 0 0 0 0 0 0 7 16 15 17 14 15 15 13 13 15 14 147 Total 34 33 33 32 31 30 31 30 34 31 319 1000 mg NaCIL Day Replicate number Total Day [1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 5 4 4 6 4 5 5 5 47 5 9 12 10 11 13 9 12 11 11 9 107 6 0 0 0 0 0 0 0 0 0 0 0 7 11 13 10 13 14 11 16 15 14 12 129 Total 24 30 25 28 31 26 32 31 30 26 283 1200 mg NaClJL Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 6 4 4 4 5 4 45 5 10 11 10 8 6 9 7 6 10 6 83 6 0 0 0 0 0 0 0 0 0 0 0 7 7 13 7 6 7 4 8 6 3 5 66 Total 22 28 21 19 19 17 19 16 18 15 194 1400 mg NaCI/L Day Re licate number Total Dy 1 2 3 4 5 6 7 8 9 10) 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 3 5 0 4 4 3 3 4 34 5 4 1 3 3 4 1 5 3 2 2 28 6 0 0 0 0 0 0 0 0 0 0 0 7 5 5 4 7 3 5 0 3 2 3 37 Total 13 10 10 15 7 10 9 9 7 9 99

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: Test dates: CdNaCICR #61 May 08-15, 2007 Reveiwed by: // Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%) Control 34 29 31 35 30 36 35 30 29 31 100 32.0 8.5 Not applicable 600 31 31 36 35 31 32 32 31 32 29 100 32.0 6.4 0.0 800 34 33 33 32 31 30 31 30 34 31 100 31.9 4.8 0.3 1000 24 30 25 28 31 26 32 31 30 26 100 28.3 10.1 11.6 1200 22 28 21 19 19 17 19 16 18 15 100 19.4 19.0 39.4 1400 13 10 10 15 7 10 9 9 7 9 90 9.9 24.9 69.1 Dunnett's MSD value: 2.700 MSD = Minimum Significant Difference PMSD: 8.4 PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Lower PMSD bound determined by USEPA (10th percentile) = 13%. Upper PMSD bound determined by USEPA (90'h percentile) = 47%. Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b). USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/8/2007 Test ID: CdNaC1CR Sample ID: REF-Ref Toxicant End Date: 5/15/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodiurn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 34.000 29.000 31.000 35.000 30.000 36.000 35.000 30.000 29.000 31.000 600 31.000 31.000 36.000 35.000 31.000 32.000 32.000 31.000 32.000 29.000 800 34.000 33.000 33.000 32.000 31.000 30.000 31.000 30.000 34.000 31.000 1000 24.000 30.000 25.000 28.000 31.000 26.000 32.000 31.000 30.000 26.000 1200 22.000 28.000 21.000 19.000 19.000 17.000 19.000 16.000 18.000 15.000 1400 13.000 10.000 10.000 15.000 7.000 10.000 9.000 9.000 7.000 9.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 32.000 1.0000 32.000 29.000 36.000 8.463 10 321000 1.0000 600 32.000 1.0000 32.000 29.000 36.000 6.421 10 111.50 75.00 32.000 1.0000 800 31.900 0.9969 31.900 30.000 34.000 4.777 10 107.00 75.00 31.900 0.9969 1000 28.300 0.8844 28.300 24.000 32.000 10.139 10 77.00 75.00 28,300 0.8844

1200 19.400 0.6063 19.400 15.000 28.000 19.009 10 55.00 75.00 19,400 0.6063
1400 9.900 0.3094 9.900 7.000 15.000 24.948 10 55.00 75.00 9.900 0.3094 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.18371189 1.035 0.76990885 0.95988629 Bartlett's Test indicates equal variances (p = 0.20) 7.30337286 15.0862722 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU Steels Many-One Rank Test 1000 1200 1095.44512 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL Skew IC05 IC10 IC 15 IC20 IC25 IC40 IC50 883.333333 53.7513901 791.75 948.262024 -2.9183 972.222222 40.9453323 888.886179 1023.68366 -0.1955 1024.7191 25.2451288 961.294823 1057.21924 -0,7793 1060.67416 20.0289083 1018.00206 1091.96511 -0.2681 1096.62921 19.2521422 1057.92603 1133.3477 0.0839 1204.21053 20.7343439 1157.77604 1242.49678 -0.0697 1271.57895 17.2179574 1225.66712 1299.12946 -0.4636 05-08-07

Environmental Testing Solutions, Inc. Statistical Analyses Used for PMSD calculation only. Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/8/2007 Test ID: CdNaClCR Sample ID: REF-Ref Toxicant End Date: 5/15/2007 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 34.000 29.000 31.000 35.000 30.000 36.000 35.000 30.000 29.000 31.000 600 31.000 31.000 36.000 35.000 31.000 32.000 32.000 31.000 32.000 29.000 800 34.000 33.000 33.000 32.000 31.000 30.000 31.000 30.000 34.000 31.000 1000 24.000 30.000 25.000 28.000 31.000 26.000 32.000 31.000 30.000 26.000 1200 22.000 28.000 21.000 19.000 19.000 17.000 19.000 16.000 18.000 15.000 1400 13.000 10.000 10.000 15.000 7.000 10.000 9.000 9.000 7.000 9.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 32.000 1.0000 32.000 29.000 36.000 8.463 10 600 32.000 1.0000 32.000 29.000 36.000 6.421 10 0.000 2.287 2.700 800 31.900 0.9969 31.900 30.000 34.000 4.777 10 0.085 2.287 2.700

1000 28.300 0.8844 28.300 24.000 32.000 10.139 10 3.134 2.287 2.700
1200 19.400 0.6063 19.400 15.000 28.000 i9.009 10 10.673 2.287 2.700
1400 9.900 0.3094 9.900 7.000 15.000 24.948 10 18.720 2.287 2.700 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.18371189 1.035 0.76990885 0.95988629 Bartlett's Test indicates equal variances (p = 0.20) 7.30337286 15.0862722 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 1000 894.427191 2.69952952 0.0843603 827.656667 6.96851852 7.3E-28 5, 54 Treatments vs D-Control 05.-08-07

Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: ,Dk Daily Chemistry: Day 1 2 _________Analyst lJ kk Concentration Parameter pH (S.U.)- f I. `),x

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Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: n\\ Concentration Parameterla pH (S.U.)

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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 INTEROFFICE SB-2A). SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TNO0264501 110 G F -FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 I YEARI MO I DAY I I YEAR MO DAY From 1 07 1 06 1 T mo0 07 06 30 ATTN: Stephanie A. Howard NO DISCHARGE [ NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 04*

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PERMIT'TEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (.INTEROFFICE SB-A SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0 TNI0026450 4 I10h T F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER M NITNR PIOD EFFLUENT YEAR MO DAY YEAR MO DAY N From 07 06 01 To 07 06 130 NOTE: Read instructions before completing this form. 004 PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE 23 CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 .PEfRMIT~ NT

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';"** *;***}* ,.-:**;.;e;: .NNU AL SAMPLE MEASUREMENT PERMIW57 >MINIMUM SAMPLE MEASUREMENT P E W SAMPLE MEASUREMENT MPERMI REQUIREMENT SAMPLE MEASUREMENT PERMIT..~> ~ SAMPLE MEASUREMENT REQUIREMENTn NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel l J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or C persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 07 13 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DA TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A). SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 116G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING PERI D EFFLUENT YEAR MO DAY YEAR MO DAY NO 01 From 07106101 I To 07 10 30 NODR NOTE: RE Form Approved. OMB No. 2040-0004 CHARGE E ]... ad instructions before completinc this form. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER J. Randy Douet Site Vice President I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations, Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVI OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 S_ _ (INTEROFFICE SB-2-A)- SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 L1 117 G F-FINAL PERMIT NUMBER [DISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard YEAR MO DAY YEAR MO DAY NO DISCHARGE ]1 From 0 =7 06L101 1 To 07t 0 13oTJ Re" NO before -3 NOTE: Read instructions before comoletinql this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1 / 30 VISUAL MEASUREMENT 01345 1 0 0 PE PASS-O

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L iREQUIREMENT-7 ~, OTTL L EFFLUENT GROSS VALUIE M0 0TL PERMIT___ .~ OIL AND GREASE VISUAL SAMPLE 0 1 / 30 VISUAL MEASUREMENT 84066 1 0 0PERMIT .:2~-****-~ REPORT YES-i1 ~***~< ~ ~ ~EE~ ,~VISUAL~ EFFLUENT GROSS VALUE WOEQUR-ME. TOTAL' NO-O PERMIT IT SAMPLE MEASUREMENT ~REQUIREMENT3 SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT PIE T R'" M.... IT,***

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~3/4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I J. Randy Douet Site Vice President I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is. to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used EPA orm33201 (EV 199) Preiousediion maybe sedPage 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _LINTEROFFICE SB-2Aj SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2640-0004 TN0026450 ]118 G F-FINAL PERMIT NUMBER ] DISCHARGE NUMBER WASTEWATER & STORM WATER fthCSklI TlDIk*,1 - cDIflf-I EFFLUENT I.- _Jr~ I~.. J.'..r FL-rxI'.JL ATTN: Stephanie A. Howard YEAR M DAY YEAR I MO DAY I

. NO DISCHARGE From o07T I mo0 07 1L06 30--]

NOTE:HRGea i o NOTE: Read instructions before completingl this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE 9** 1 MEASUREMENT 00300 1 0 0 PFRMIT20~ ~ ~ ~ ~<K GLiW~~ EFFLUENT GROSS VALUE REQUI$EMEN.1'- -DAILY>MN VJE E*3/4'

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MGIL TWItQif -GRAB:' EFFLUENT GROSS VALUE REQUIREMENT DAILY MX< WEEK SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 0 RER,MIT~ i~ ~ ~ -~ 1 V 0,~ MLIL A O'NCE/ GRAB EFFLUENT GROSS VALUE REQUIREMENT~ ~ ~ .~ ~ DALYM 4 M4ONTH FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 PEIRMITA:- .EPORT ..R.. M MGD 'i** ONE/.*!,.EstIMA., MEASU1IREMENT._R iR'-O~ EFFLUENT GROSS VALUE 'EURMN.>DAILY"MX-

BATCH, SAMPLE MEASUREMENT SAMPLE MEASUREMENT 3/4REOUIREM ENT SAMPLE MEASUREMENT

-REQUIREMENTO ,~i1 .. '..."K NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 07 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}

ML071990319

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ML071990319

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Dear Mr. Hannah:

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