ML070390152
| ML070390152 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 02/05/2007 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| TN0026450 | |
| Download: ML070390152 (237) | |
Text
{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 February 5, 2007 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534
Dear Mr. Chip Hannah,
SEQUOYAH NUCLEAR PLANT.(SQN)-- 2006 BIOCIDE/CORROSION TREATMENT PLAN' ANNUAL REPORT- NPDES PERMIT NO. TN0026450 In accordance with Part IV.B. of NPDES Permit No. TN0026450, SQN developed a Biocide/Corrosion Treatment Plan .(B/CTP) that was approved by the Division of Water Pollution Control on April 27, 2005 for the specific and limited application of oxidizing biocides, non-oxidizing biocides, dispersants, surfactants', corrosion inhibiting chemicals, and'detoxification chemicals at SQN. The use of these chemicals is necessary to ensure the safe operation of the facility.. The B/CTP approval requires that "anrfually, a report shall be submitted to the Division presenting the biomonitoring data for tests conducted during treatments, a summaryof all analytical results (daily maximum, daily average, number of samples), the approximate duration in hours of each chemical used, quantity in pounds of each chemical used, and any minor changes that have occurred to the plan. The report shall be submitted to the Enforcement and Compliance Section in Nashville and to the Chattanooga field office by February 15 of the year following the reporting year. Also; in order to compare reliability of the mass balance calculations with the methylene chloride extraction method, SON shall .compare both methods used for analyses of the effluent and report to the Division." SON compared the results of both methods (mass balance .calculations and the methylene chloride, extraction method) used for analyses of the effluent. However, SQN did not detoxify the effluent during any non-oxidizing biocide treatments in 2006.: A total of 36 samples from the Diffuser effluent (Outfall 101) have been analyzed by each method, see summary on the following page.
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Printed on recycsed paper
Analytical Results Analytical Results H-1 50M Mass Balance Calculations (mg/L) Methylene Chloride Extraction (mg/L)
# of of Month Maximum Average Limit samples Maximum Average Limit samples May..-. 0.036 0.035 0.05 8 <0.02 <0.02 0.05 8.
June 0.036 0.035 0.05 4 <0.02 <0.02 0.05 -4 ..July 0.033 0.033 0.05 2 <0.02* <0.02 0.05 2 August 0.033 0.033 0.05 6 <0.02 <0.02 0.05 *6 October 0.035 0.033 0.05 5 ý<0.02 <0.02. 0.05 5 November 0.037 0.036 0.05 11 <0.02 <0.02. 0.05 11 May-Nov. 36 36 From January 1;2006 through December 31, 2006, SQN did not inject the following chemicals into any system (ERCW A, ERCW B, RCW): Nalco H-1 30M (non-oxidizing biocide), Betz
Dearborn Spectrus CT1 300 (non-oxidizing biocide),
nor Nalco Coagulant Aid-35.(detoxification). MSW 101 was added to the.RCW system in September 2006. (reference the March 27, 2006 and August 15, 2006 letters sent to Ms. Pamala Myers from Stephanie A. Howard). Enclosed is the 2006 Biocide/Corrosion Treatment Plan. Annual Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if youhave any questions-or comments. Sincerely, - Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah'Nuclear Plant Enclosure cc"(Enclosure): Edward M. Polk, Jr., P.E. Chattanooga Environmental Assistance Center Manager, Permit Section Division of Water Pollution Control State of Tennessee State Office Building, Suite 550 Dept. of Environment and Conservation 540 McCall*ie Avenue
- Division of Water Pollution Control Chattanooga, Tennessee 37402-2013 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report H 150M' Duration in Quantity in Pounds Hours Analytical Results Analytical Results (lbs/day) (hrs/day) mass balance calculations methylene chloride extraction (mg/L) (mg/L)
#of # of IDays in Month I Maximum Average Limit Maximum Maximum Average Limit samples I Maximum Average Lirriit samples I Service January 0 0 855 0.00 0.000 0.000 0.05 0 0.00 0.00 0.05 0 0 February 0 0 855 0.00 0.000 0.000 0.05 0 0.00 *0.00 0.05 0 0 March 0 855 0.00 0.000 0.000 0.05- 0 0.00 0.00 0.05 0 0 0
April 0 855 0.00 0.000 0.000 0.05 0 0.00 0.00 0.05 0 .0 May 452 303 855 24.00 0.036 0.035 0.05 8 <0.020 <0U020 0.05 8 8 June 461 288 855 24.00. 0.036 0.035 0.05 4 <0.020 <0.020 0.05- 4 4 July 248 237 855 13.75 0.033 0.033 0.05 2 <0.020 <0.020 0.05 2 2 August 299 240 855 16.58 0.033 0.033 0.05 6 <0.020 <0.020* 0.05 6 .6 September 0 0 '855 0.00 0.000': 0.000 0.05 0 0.00 0.00. 0.05 0 0 October 290ý 250 855 15.92 0.035 0.033 0.05 5 <0.020 <0.020 0.05; 5 .5 November 481 379 855 24.00 0.037 0.036 0.05 11 <0.020 <0.020 0.05 11 11 0.00 December 0 0 855 0.00 0.000 0.000 0.05 0 0.00 0.05 0 0 Jan. - Dec. 36 36 36 Perthe B/CTP approval: "Whole Effluent toxicity testing (biomonitoring).of Outfall.101 and Outall 110 shall be undertaken once'per year when non-oxidizing biocides are being used." Outfall 101: Toxicity was sampled November 12n17, 2006.,
.Test Results: Pimephales promelas: IC25 >1 00%
Ceriodaphniadubia: IC25> 100% Outfall 110: There has been no discharge from Outfall 11.0 January - December 2006.
H-150M mass methylene injection start injection end. duration total balance chloride, H-150M system time time in hours quantity calc. extraction number/type of samples 855 lbs/day 0.05 mg/L 0.05 mg/L 05/17/2006 ERCW B 1030 2400 13.50 243.34 0.0345 <0.020 1 Comp. 05/17/06 @ 1039 - 05/18/06 @ 0939 05/18/2006 ERCW B 0000/1010 0826/2400 22.26 401.24 0.0344 <0.020 1 Comp. 05/18/06 @ 0955 - 05/19/06 @ 0855 05/19/2006 ERCW B, 0000 1200 12.00 216.30 0.0340 <0.020 1 Comp. 05/19/06 @ 1042 - 05/20/06.@ 0942 05/22/2006 ERCWA 1315 .2400 10.75 193.77 0.0340 <0.020 1 Comp. 05/22/06:@ 0830 -:05/23/06 @0730 05/23/2006 ERCW A 0000 240.0 24.00 432.60 0.0340 <0.020 1 Comp. 05/23/06 @ 0757 - 05/24/06 @ 0657 05/24/2006 ERCWA 0000 1315 13.25 238.83 0.034 <0.020 1 Comp. 05/24/06 @ 0813 - 05/25/06 @ý0713 05/30/2006 RCW 1120 2400 12.67 243.60 0.0362 <0.020 1 Comp. 05/30/06 @ 0755 - 05/31/06 @ 0655 05/31/2006 RCW 0000/1500 1430/2400 23.50 451.83 0.036 <0.020 1 Comp. 05/31/06.@ 0725 - 06/01/06 @ 0625 mass methylene injection start injection end duration total balance Chloride H-150M system time time in hours quantity calc. extraction number/type of samples 855 lbs/day 0.05 mg/L 0.05 mg/L 06/01/2006 RCW 0000 2400 24.00 461.45 0.036 <0.020 1 Comp. 06/01/06 @ 0824'- 06/02/06 @ 0724 06/02/2006 RCW 0000 1115 11.25 216.30 0.036 <0.020 1 Comp. 06/02/06 @ 0821 - 06/03/06 @ 0721 06/26/2006 ERCW B .1108 2400 12.87 231.98 0.033 <0.020 1 Comp. 06/26/06 @ 0737 - 06/27/06 @0637 06/27/2006 ERCW B 0000 1330 13.50 243.34 0.033 <0.020 1 Comp. 06/27/06 @ 0727 - 06/28/06 @ 0627 mass methylene injection start injection.end duration total balance chloride H-150M system time time in hours. quantity calc. extraction number/type of samples 855 lbs/,day 0.05 mg/L. 0.05 mg/L 07/05/2006 ERCWA 1015 2400 13.75 247.85 0.033 <0.020 1 Comp. 07/05/06 @ 0737- 07/06/06 @ 0637 07/06/2006 ERCWA 0000 1235 12.58 226.75 0.033 <0.020 1 Comp. 07/06/06 @ 0741 - 07/07/06 @ 0641
H-1 50M mass methylene. injection start injection end duration total balance chloride H-150M system time time in hours quantity calc. extraction number/type of samples 855 lbs/day 0.05 mg/L 0.05 mg/L 08/07/2006 ERCW B 1015 2400 13.75 247.85 0.033 <0.020 1 Comp. 08/07/06 @ 0715 - 08/08/06 @ 0615 08/08/2006 ERCW B 0000 1326 13.43 242.08 0.033 <0.020 1 Comp. 08/08/06 @0659 - 08/09/06 @ 0559 08/15/2006 ERCWA 1016 2400 13.73 247.49 0.033 <0,020 1 Comp. 08/15/06 @ 0738 - 08/16/06 @0638 08/16/2006 ERCWA 0000 1225 12.42 223.87 0.032 <0,020 1 Comp. 08/16/06 @ 0710 - 08/17/06 @ 0610' 08/23/2006 RCW 1406 2400 9.90 178.45 0.033 <0,020 1 Comp. 08/23/06 @0910 - 08/24/06 @ 0635. 08/24/2006 RCW 0000 1635 16.58 298.86 0.032 <0.020 1 Comp. 08/24/06 @:0636 .08/25/06 @ 0536 mass methylene injection start injection end duration' total balance chloride H-150M system time time -in hours quantity calc. extraction number/type of samples.; 855 lbs/day 0.05 mg/L 0.05 mg/L 10/02/2006 ERCW B 1015 2400 13.75 247.85 0.032 <0.020 1 Comp. 10/02/06 @ 0837 - 10/03/06 @ 0737 10/03/2006 ERCW B 0000 1330 13.50 243.35 0.032 <0.020 1 Comp. 10/03/06.@ 0744 - 10/04/06 @ 0844 10/24/2006 ERCWA 0810 2400 15.83 285.34 0.033 <0.020 1 Comp. 10/24/06 @ 081,5- 10/25/06 @ 0715 10/25/2006 ERCWA 0000 1015 1.0.25 184.76 0.033 <0.020 1 Comp. 10/25/06 @ 0737 - 10/26/06 @ 0637 10/31/2006 RCW 0805/1308 1308/2400 15.92 290.46 0-035 <0.020 1 Comp. 10/31/06 @ 0820 - 11/01/06 @ 0720 mass methylene injection start injection end duration total balance chloride H-150M system time . time in hours quantity calc. extraction number/type of samples 855. lbs/day 0.05 mg/L 0.05 mg/L 11/01/2006 RCW 0000/1320 1245/2400 23.42 420.57 0.033 <0.020 1 Comp. 11/01/06 @ 0730 - 11/02/06 @ 0630 11/02/2006. RCW 0000 '.2400 24.00 432.60 0,033 <0.020 1 Comp.1 11/02/06 @ 0647 - 11/03/06 @ 0547, 11/0.3/2006 RCW 0000 2400 24:00 432.60 0.033 <0.020 1 Comp. 11/03/06 @ 0700 - 11/04/06 @ 0600 11/06/2006 ERCW A 1445 2400 9.25 185.26 0.037 <0.020 .1 Comp. 11/06/06 @-0808 - 11/07/06 @ 0708 11/07/2006 ERCWA 0000 2400 24.00 480.67 0.036 <0.020 1 Comp. 11/07/06 @ 0746Q- 11/08/06 @ 0646 11/08/2006 ERCWA )000/1145/1355 00/1335/2400 20.91 418.78 . " 0.036 <0.020 1 Comp. 11/08/06.@ 0908- 11/09/06 @ 0808 11/09/2006 ERCWA 0000 1750 17.83 357.10 0.036 <0.020 1 Comp. 11/09/06 @ 0838 - 11/10/06 @ 0738 11/13/2006 , ERCWB 1155 2400 12.08 241.94 0.037 <0.020 1 Comp. 11/113/06 @ 0905- 11/14/06 @ 0805 .11/14/2006 ERCW B 0000/0914 0812/2400 22.97 460.04 0.037 <0.020 1 Comp. 11/14/06 @ 0816' 11/15/06 @ 0716 11/15/2006 ERCWB 0000 2400 24.00 480.67 0.037 <0.020 1 Comp. 11/15/06@ 0730- 11/16/06 @ 0630 11/16/2006 ERCWB 0000 1300 13.00 ., 260.36 0.037 <0.020 1 Comp. 11/16/06 @.0724- 11/17/06 @ 0624
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report" Biodetergent 73551 Quantity in Pounds Duration in Hours Analytical Results (lbs/day) (hrs/day)*. mass balance calculations (mg/L)
# of Days in Month Maximum Average Limit Maximum Limit Maximum Average Limit samples Service January 7 5 50 0.5 0.5 0.012 0.011 2.0 I 11 11 0.5 0.011 2.0 -3 " 3 February 5 5 50 0*.5 0.012 March 0 0 50 0.0 0.5 0.000 0.000 2.0 0 0 April 5 5 50 0.5 0.5 0.019 0.017 2.0 8 8 May 0 0 50 0.0 0.5 0.000 0.000 2.0 0 0 June 8 7 50 0.5 0.5 0.012 0.011 2.0 10 .10 July 8_ 5 50 0.5 0.5 0.011 0.010 2.o .13 13,'
August 9 5 50 0.5 0.5 0.015 0.012 2.0 12 12 September 13 7 .50 0.5 0.5 0.015 0.014 2.0 12 12 October 12 6 50 0.5 0.5 0.016 0.01.5 2.0 11 11 November 9 9 50 '0.5 0.5 0.015 0.015 2.0 1 1 0.5 91 December 8 6 50 0.5 0.026 0.025 2.0 9 Jan. - Dec. 90 90
- Treatment durations shall not exceed 30 minutes at 2-3 times per week into ERCW Train A, ERCW Train B, and RCW systems.
Per the B/CTP approval:. "Frequency of use would be appoximately 208 days per year and duration of use would be about 0.5 hours perday with a maximum daily usage of 50 lbs/day for all three-injection points."
Biodetergent 73551 system injection start time injection end.time duration in hour total quantity mass balance calc. 50 Ibs/day 2.0 mg/L 01/01/2006 n/a hor; lb/a 01/02/2006 N/A 011O3!2006' ERCWA 1015 1045 0.50 ERCW B 1045 1115 0.50 RCW 1210 1239 0.48 7.27 0.012 01/04/2006 ERCWA 845 915 . 0.50 ERCW B 915 945 0.50 RCW 1050 1119 0.48 , 7.27 0.012 01/05/2006 N/A, 01/06/2006 N/A 01/07/2006 N/A, : 01/08/2006 Ný/A'-* 01/09/2006 ERCW A 900 930 0.50 ERCW B 930 1000 0.50 4.36 0.008 01/10/2006 N/A 01/11/2006 N/A 01/12/2006 RCW 900 929 0:48 2.91 0.012 01/13/2006 ERCW A 925 955 0.50 ERCW B 855 925 0.50 RCW ' 1030 1059 0.48 7.27 0.012 01/14/2006 N/A 01/15/2006 N/A
.01/16/2006 N/A -_
01/17/2006 N/A 01/18/2006 . RCW 1020 1049 0.48 2.91 0.012 01/19/2006 RCW 1040 1109 0.48 2.91 0.012
'01/20/2006 ERCWA 835 905 0.50 ERCW B 905 935 0.50 4.36 0.008 01/21/2006 N/A ,_____
01/22/2006 N/A 01/23/2006 ERCW A 1110 .1140 0.50 ERCW B .1140 1210 0.50 RCW . 1300 1329 0.48 7.27 " 0.012 01/24/2006 ERCWA 930 1000 0.50 ERCW B "1000 1030 . 0.50 RCW 1125 1154 0.48 7.27 0.012 01/25/2006 N/A 01/26/2006 N/A 01/27/2006 N/A 01/28/2006 N/A 01/29/2006 N/A 01/30/2006 N/A 01/30/2006 N/A 01/31/2006 RCW 1030 1059 0.48
- 2.91 0.012
W IýMLý HFIIt I .JJý I Biodetergent 73551 system injection start time injection end time duration in hour total quantity mass balance calc. 50 lbs/day 2.0 mg/L 02/01/2006 ERCW A 1230 1300 0.50 ERCW B 1300 1330 0.50 5.42 0.011 02/02/2006 RCW 1025 1055 0.50 .3.03 0.012 02/03/2006 ERCW A 905 935 0.50 ERCW B 935 1005 0.50 5.42 0.01 02/04/2006 N/A 02/05/2006 N/A "_ _ __ 02/06/2006 N/A 02/07/2006 N/A 02/08/2006 N/A 02/09/2006 N/A 02/10/2006 N/A 02/11/2006 N/A 02/12/2006 N/A 02/13/2006 N/A 02/14/2006 N/A 02/15/2006 N/A 02/16/2006 N/A 02/17/2006 N/A 02/18/2006 N/A 02/19/2006 N/A 02/20/2006 N/A 02/21/2006 N/A "_ _ _ 02/22/2006 N/A .. 02/23/2006 N/A 02/24/2006 N/A 02/25/2006 N/A 02/26/2006 N/A 02/27/2006 N/A 02/28/2006 N/A
LJIUUeLeIl gal It I .J.J I Biodetergent 73551 system injection start time injection end time duration in hours total quantity mass balance calc. 50 lbs/day 2.0 mg/L 04/01/2006 n/a _ ._ 04/02/2006 n/a 04/03/2006 n/a 04/04/2006 ERCWA 830 900 0.50 ERCW, B 900 930 0.50 RCW . 1020 1050 0.50 5.41 0.012 04/05/2006 n/a "_.__ _ _ _ 04/06/2006 ERCW A 1115 1145 0.50: ERCW B 1145 1215 0.50 " SRCW 935 1005 0.50 5.41 0.012 04/07/2006 n/a " 04/08/2006 n/a 04/09/2006 n/a 04/10/2006 " N/A 04/11/2006 ERCW A 1250 1320 0.50 ERCW B 1320 1350 0.50 RCW __ 940 . 101.0 0.50 5.31 0.017 04/12/2006 N/A 04/13/2006 ERCW A, 1015 1045 0:50 ERCW B1 1045 1115 0.50 RCW" 915
- 945 0.50 5.31 0.02 04/14/2006 N/A 04/15/2006 N/A 04/16/2006 N/A 04/17/2006 ERCW A 900 930 0.50 ERCW B 930 1000 0.50 RCW 1050 1120 0.50 5.31 0.02.
04/18/2006 n/a 04/19/2006 ERCW A 915 945 0.50 ERCW B .945 1015 0.50 RCW .1125 1.155 0.50 5.31 0.02 04/20/2006. n/a 04/21/2006 n/a 04/22/2006 n/a 04/23/2006 n/a 04/24/2006 RCW 1048 1118 0.50 3.03 0.019 04/25/2006 N/A
- ' 04/26/2006 ERCWA 955 .1025 0.50 ERCW B 1025 1055 0.50 RCW 1155 1225 0.50 5.31 0.019 04/27/2006 N/A 04/28/2006 N/A 04/29/2006 N/A 04/30/2006 N/A __
Biodetergent 73551 system injection start time injection end time duration in hours total quantity mass balance calc. 50 lbs/day 2.0 mg/L 06/01/2006 ERCW A 950 1020 0.50 ERCW B 1020 1050 0.50 4.56 0.008 06/02/2006 ERCW A 1440 1510 0.50 ERCW B 1510 1540 0.50 4.56 0.008 06/03/2006 n/a, 06/04/2006 n/a 06/05/2006 n/a 06/06/2006 *ERCW A 850 920 0.50 ERCW B 920 950 0.50 RCW 1055 1125 0.50 8.14 0.012 06/07/2006 n/a 06/08/2006 n/a 06/09/2006 ERCW A 1135 1205 0.50 ERCW B 1205 1235 0.50 RCW 1330 1400 0150 8.14 0.012 06/10/2006 n/a 06/11/2006 n/a 06/12/2006 n/a 06/13/2006 n/a 06/14/2006 ERCW A 940 .1010 0.50 ERCW B 1010 1040 0.50 RCW 1215 1245 0.50 7.77 0.011 06/15/2006 n/a __ _ 06/16/2006 ERCWA. 1100 1130 0.50 ERCW B 1130 1200 0.50 RCW 1300 1330 0.50 . 7.59 0.011 06/17/2006 n/a 06/18/2006 n/a 06/19/2006 n/a 06/20/2006 n/a 06/21/2006 ERCWA 900 930 0.50
.ERCW B 930 1000 0.50 .
RCW 1015 1045 0.50 7.59 . 0.01.1
, 06/22/2006 n/a 06/23/2006 ERCW A . 900 930 0.50 ERCW B - 930 1000 0.50 -. _ RCW 1020 1050 0.50 7.59 0.011 06/24/2006 n/a 06/25/2006 n/a 06/26/2006 n/a 06/27/2006 n/a 06/28/2006 RCW 1005 1035 0.50 3.03 0.011 06/29/2006 n/a 06/30/2006 ERCW A 1110 " 1140 0.50
_ ERCW B 1140 1210 0.50
- RCW 1250 .1320 0.50 7.59 0.011
L..IvuvclI yei It. f .J.j I Biodetergent 73551 system injection start time injection end time duration in hours total quantity mass balance calc. 50 Ibs/day 2.0 mg/L 07(01(2006 ERCW A 1125 1155 0.50 ERCW B 1155 1225 _ 0.50 _ 4.56 0.00.8 07/02/2006 n/a 07/03/2006 ERCWA 1125 1155 .0.50 ERCWB 1155 1225 o.50 4.56 0.008 07/04/2006 n/a 07/05/2006 n/a _ _ _ _
- 07/06/2006 RCW . 935 1005 0.50 ._3.03 0.011 07/07/2006 RCW 935 1005 0.50 3.03 0.011 07/08/2006 n/a 07/09/2006 n/a 07110/2006 n/a 07/11/2006 n/a 07/12/2006 n/a 07/13/2006 n/a 07/14/2006 ERCW A 935 1005 0.50 ERCW B 1005 1035 0.50 RCW 1205 1235 0.50 7.59 0.011 07/15/2006 ERCW A 905 935 0.50 ERCWB 935 .1005 0.50 4.56 0.008 07/16/2006 RCW 925 955 0.50 3.03 0.011 07/17/2006 n/a 07/18/2006 ERCW A 920 950 0.50 ERCWB 950 1020 0.50 RCW 1625 1655 0.50 7.59 0.011 07/19/2006 n/a 07/20/2006 n/a 07/21/2006 ERWA 915 945 0.50 ERCW B 945 1015 0.50 RCW 1115 1145 0.50 7.59 0.011 07/22/2006 n/a _
07/23/2006 n/a 07/24/2006 n/a _ . 07/25/2006 RCW 920 950 0.50 3.03 0.011 07/26/2006 . ERCW A 850 920 0.50 ERCW B 920 950 0.50 4.56 0.009 07/27/2006 RCW 1100 1130 0.50 3.03 0.011 07/28/2006 ERCWA 945 1015 0.50 ERCW B 1015 1045 0.50 4.56 0.009
.07/29/2006
- n/a 07/30/2006 n/a 07/31/2006 n/a
LJI'.I I L I ý I Biodetergent 73551 system . injection start time injection end time duration in hour, total quantity mass balance calc. 50 lbs/day .2.0 mg/L 08/01/2006 n/a 08/02/2006 n/a. 08/03/2006 ERCW A 1225 1255 05. ERCW B 1255 1325 0.50 4.56 0.009 08/04/2006 ERCW A. 1005. 1035 0.50 ERCW B 1035 1105 . 0.50 RCW 1140 1210. 0.50. 7.59 0.011 08/05/2006 n/a 08/06/2006 n/a 08/07/2006 n/a 08/08/2006 n/a 08/09/2006 n/a 08/10/2006 RCW 933 1003 0.50 3.03 0.011 08/11/2006 n/a 08/12/2006 RCW ; 910 940 0.50 3.98 0.014 08/13/2006 n/a 08/14/2006 n/a 08/15/2006 n/a 08/16/2006 RCOW 855 925 0.50 3.98 .0.015 08/17/2006 n/a 08/18/2006 ERCW A 930 1000 0.50 ERCW B 1000 1030 0.50 RCW 1104 1134 0.50 8.54 0.014 08/19/2006 ERCW A 820 850 0.50 ERCW B 850 920 . 0.50 4.56 0.008 08/20/2006 n/a 08/21/2006 n/a 08/22/2006 ERCW A 850 920 0.50 ERCW B 920 950 .0.50 4.56. 0.008 08/23/2006 n/a 08/24/2006 n/a 08/25/2006 ERCW A 950 1015 0.42 ERCW B 1015 1.045 0.50 4.19 0.008 08/26/2006 RCW 900 930 0.50 3.98 0.014 08/27/2006 RCW 925 955 0.50 3.98 0.015 08/28/2006 n/a
'08/29/2006 ERCW A 948 1018 0.50 ERCW B 1018 1048 0.50 RCW 830 900 0.50 8.73 0.015 08/30/2006 n/a 08/31/2006 n/a
-- ". &.1 1L , ýý' I Biodetergent 73551 system injection start time injection end time duration in hour, total quantity L mass balance calc.
50 lbs/day 2.0 mg/L 09/01/2006 ERCW A 952 1022 0.50 ERCW B 1022 1052 0.50 RCW 1145 1215 0.50 8.73 0.015 09/02/2006 n/a 09/03/2006 n/a 09/04/2006 n/a 09/05/2006 n/a 09/06/2006 ERCW A 935 1005 0.50 ERCW B 1005 1035 0.50 RCW' 1135 1205 0.50 8.73 0.015 09/07/2006 n/a 09/08/2006 ERCWA 940 1010 .0.50 ERCW B 1010 1040 0.50 RCW 1130 1200 0.50 8.73 0.015 09/09/2006 n/a 09/10/2006 n/a -_ 09/11/2006 n/a 09/12/2006 RCW 1012 1042 0.50 4.17 0.015 09/13/2006 n/a 09/14/2006 RCW 905 935 0.50 4.17 0.015 09/15/2006 n/a 09/16/2006 ERCWA . 805 835 0.50 ERCW B. 835 905 0.50 4.56 0.008 09/17/2006 ERCW A 900 930 0.50 ERCW B 930 1000 0.50 4.56 0.008 09/18/2006 n/a 09/19/2006 n/a 09/20/2006 n/a 09/21/2006 ERCW A 910 940 0.50 ERCW B 940 1010 0.50 RCW 1103 1133 0.50 9.95 0.015 09/22/2006 ERCW A 840. 910 0.50 ERCW B 910 940 0.50 RCW 1025 1055 0.50 11.19 0.015 09/23/2006 n/a 09/24/2006 n/a 09/25/2006 n/a 09/26/2006 ERCW A 920 950 0.50 ERCW B 950 1020 0.50 RCW 1107 1137 .0.50 . 12.51 0.015 09/27/2006 n/a ._ __' 09128/2006 RCW 931 1001 0.50 4.17 0.015 09/29/2006 n/a 09/30/2006 ERCW A 855 925 0.50 ERCW B 925 955 0.50 8.35 0.015
S.dS..tS~S~ IL S SJSd Sd I Biodetergent 73551 system injection start time injection end time duration in hours .. total quantity [mass balance calc. 50 lbs/day 2.0 mg/L 10J0112006 nla ,__ 10/02/2006 n/a . 10/03/2006 RCW 937 1007 0.50 4.17 0.015 10/04/2006 n/a __-_' __ 10/05/2006 n/a -_ _ __ _ _ 10/06/2006 ERCVV A. 850 920 0.50 ERCW B 920 950 0.50 RCW 1045 1115 0.50 11.75 0.015 10/07/2006 n/a 10/08/2006 ERCW A __850 920 0.50 ERCW.B 920 950 0.50 7.58 0.014 10/09/2006 RCW 820 850 0.50 4.17 0.015 10/10/2006 n/a 10/11/2006 ERCW A 1010 1040 .0.50 ERCWB. 1040 1110 0.50 6.26 0.011 10/12/2006 RCW 1030 1100 0.50 4.17 0.015 10/13/2006 n/a 10/14/2006 n/a 10/15/2006 n/a 10/16/2006 RCW. 1124 1154 - 0.50 4.17 0.015 10/17/2006 n/a 10/18/2006 RCW . 955 1025 0.50 4.17 . 0.015 10/19/2006 n/a 10/20/2006 n/a _ 10/21/2006 n/a 10/22/2006 n/a 10/23/2006 RCW 955 1025 0.50 4.17 .. 0.015 10/24/2006 n/a 10/25/2006 RCW 915 945 0.50 .4.17 0.015 10/26/2006 n/a 10/27/2006 n/a 10/28/2006 n/a 10/29/2006 n/a 10/30/2006 ERCW A 850 920 0.50 ERCW B 920 950 .0.50 8.54 . 0.016 10/31/2006 n/a '___ _
;IkJUt:LUIYUI It I ;IJ.J I Biodetergent 73551 system injection start time injection end time duration in hours total quantity mass balance calc.
50 lbs/day 2.0 mgIL 11/01/2006 n/a 11/02/2006 ERCW A 835 905 0.50 ERCW B 905 935 0.50 8.54, 0.015 11/03/2006 n/a 11/04/2006 n/a ___ 11/05/2006 n/a 11/06/2006 n/a 11/07/2006 n/a 11/08/2006 n/a ,_- 1.1/09/2006 -. n/a 11/10/2006 n/a 11/11/2006 n/a 11/12/2006 n/a _ -_ 11/13/20061 n/a 11/14/2006 n/a ,11/15/2006 n/a _ _ _ __ _ 11/16/2006 n/a 11/17/2006 n/a
- 11/18/2006 n/a 11/19/2006 n/a 11/20/2006 n/a*
11/21/2006 n/a _ 11/22/2006 n/a 11/23/2006 n/a 11/24/2006 n/a 11/25/2006 n/a 11/26/2006 n/a. 11/27/2006 in/a "_" 11/28/2006 n/a 11/29/2006 n/a 11/30/2006 n/a _
DIvUt::tIy:IIL /I OZ I Biodetergent 73551 system injection start time injection end time [_50 duration in hours- total lbs/day quantity mass2.0 balance
'mg/L calc.
12/01/2006 n/a "_ _ , 12/02/2006 n/a .__ 12/03/2006 n/a 12/04/2006 n/a 12/05/2006, n/a "_ _ _ 12/06/2006 n/a ._"_- 12/07/2006 RCW 940 1010 0.50 : 4.17 0.026 12/08/2006 n/a 12/09/2006 n/a 12/10/2006 n/a 12/11/2006 RCW 845 915 0.50 4.17 0.025 12/12/2006 ERCW A 925 955 0.50 ERCW B 955 1025 0.50 8.34 0.026 12/13/2006 RCW 905 935 0.50 4.17 0.026 12/14/2006 ERCW A 840 910 0.50 __ERCW B 910 940 0.50 8.34 0.026 12/15/2006 n/a 12/16/2006 -.n/a _ _ _ 12/17/2006 n/a 12/18/2006 ERCW A 840 910 0.50 ERCW B 910 940 0.50 8.34 0.026 12/19/2006 RCW 850 920 0.50 4.17 0.026 12/20/2006 ERCW A 850 920 0.50 ERCW B 920 950 .0.50 8.34 0.026 12/21/2006 n/a 12/22/2006 n/a 12/23/2006 n/a 12/24/2006 n/a 12/25/2006 n/a 12/26/2006 n/a 12/27/2006 . ERCWA 1220 1250 0.50
- _ ERCW B 1250 1320 0.50 8.34 0.016 12/28/2006 : n/a _ "__
12/29/2006 n/a _ 12/30/2006, n/a 12/31/2006 n/a "
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report PCL 222 Copolymer Duration in Quantity in Pounds .Hours Analytical Results (lbs/day) (hrs/day) mass balance calculations (mg/L)
# of Days in Month .Maximum Average Limit' Maximum Maximum Average Limit samples Service January 259 133 760 24.00 0.021 0.011 0.2 31 31 February 28 26 760 24.00 0.005 0.002 0.2 7 7 March 56 40 760 24.00 0.005 0.005 0.2 2 2 April 56 49 760 24.00 0.008 0.007 0.2 26 26 May 0 '0 760 0.00 0.000 0.000 0.2 0 0 June 171 112 760 24.00 0.014 0.010 0.2 24 24 July 169 153 760 24.00 0.014 0.013 0.2 31 31 August 169 111 760 24.00 0.013 0.010 0.2 30 '30 September 113 108 760 24.00 0.009 0.008 0.2 26 26 October 113 75 760 24.00 0.008 0.008 0.2 2 2 November 0 0 760 0.00 0,000 .0.000 0.2 0 0 December 0 0 760 0.00 o.ooo, o.booo 0.2 0 0 Jan. - Dec. 179 179 Per the B/CTP approval: "PCL-222 is injected into the ERCW Train A & B system only during warm weather months." PCL-222 was injected in January due to the average ambient temperature being above 35 degrees Fahrenheit.
.TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report PCL 222 Phosphate. Duration in Quantity in Pounds Hours Analytical Results (lbs/day) (hrs/day) mass balance calcualtions (mg/L)
# of Days-in Month Maximum Average Limit Maximum Maximum Average. Limit' samples Service January 755 389 2280 24.00 0.061 0.033 0.2 31 31 February 82 77 2280 24.00 0.013 0.007 0.2 7 7 March 165 116, 2280 24.00 0.013 0.013 0.2 2 2 April 165. 143 2280 24.00 0.023 0.019 0.2 26 26 May 0 0 2280 0.00 0.000 0.000 0.2 0 0 June 500 326 2280 24.00 0.041 0.029 0.2 24 24 July 494 447 2280 24.00 0.041 0.038 0.2 31 .31 August 494 324 2280 24.00 0.039 0.028 0.2 30 30 September 330 315 2280 24.00 0.025 0.025 0.2 26 26 October 330 ,219 2280 24.00 0.025 0.025 0.2 2 2 November 0 0 2280 0.00 0.000 0.000 0.2 0 0 December 0 0 2280 0.00 o0.000 0.000 0.2 0 0 Jan. - Dec. 179 179.
Per the B/CTP approval: "PCL-222 is injected into the ERCW Train A & B system only during warm weather months." PCL-222 was injected in January due to the average ambient temperature being above 35 degrees Fahrenheit. :
PCL 222 injection injection injection injection duration in PCL-222 system start time end timte start time end time hours COPOLYMER PHOSPHATE total quantity mass balance calc. total quantity mass-balance calc.
- - 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 01/01/2006. ERCW A & & RCW 00 00 24 00 24.00 258.76 0021 755.45 0.061 01/02/2006 ERCWA & B & RCW 0 00 24 00 24.00 258:76 0.021 755.45 0.061 01/03/2006 ERCWA& B 0 24
-00 000 24.00 ..
RCW 00100 12 05 17 05 24 00 19.00 234.25 0.021 683.91 0.06 01/04/2006 ERCW A& B 00-100 24 00 24.00 RCW 00 00 10 25 15 35 24 00 18.83 233.47 0.021 681.62 0.061 01/05/2006 ERCW A & B & RCW 00 :100 24 00 1 24.00 258.76 0.021 755.45 0.061 01/06/2006 ERCW A & B & RCW 00 24 _00 00 24.00 258.76 0.021 755.45 0.061 01/07/2006 ERCW A & B & RCW 00 00. .24 00 24.00 258.76 0.021 755.45 0.061 01/08/2006 ERCW A & B & RCW 00 00 24 00 24.00 258.76 0.021 755.45 0.061 01/09/2006 RCW 00 00 24 00 24.00 117.62 0.009 .. 343.39 0.028 01/10/2006 RCW 0 00 24 00 24.00 117.62 0.009 343.39 0.028 01/11/2006 RCW .00 00 24 00 2400 117.62 0.009 343.39 0.028 01/12/2006 RCW 00 00 08 30 13 28 24 00 1903 93.26 0.009 272.28 0.028 01/13/2006 RCW 00 00 10 05 14 55 24 00 19.17 93.93 0.009 274.23 0:027 01/14/2006 RCW 00 00 24 00 1 2400 117.62 0.009 343.39 0.027 01/15/2006 RCW 00 100 24 00 24.00 117.62 0.009 343.39 0.027 01/16/2006 RCW 00 100 24 00 400 117.62 0.009 343.39 0.027 01/17/2006 RCW 00:00 24:00 2400 117.62 0.01 343.39 0.028 01/18/2006' RCW 00 09
)00 50 15 70 24 00 1867 98.76 0.01 288.34 0.03 01/19/2006 RCW 00 100 101 15 15 : 35 24 00 1867 91.5 0.01 267.13 0.028 01/20/2006 RCW 00 100 241 00 1 24.00 122.32 0.01 357.12 . 0.029 01/21/2006 RCW 00 00 24 00 "2400 122.32 0.01 357.12 0.028 01/22/2006 RCW 00 00 24 00 24.00 122.32 0.01 357.12 0.028 01/23/2006 RCW 00 00 12 35 17 : 50 24 :100 18.75 95.56 o0.01 279 0.029 01/24/2006 RCW 00 00 11 00 16 10 24 00 1883 92.28 0.01 269.42 0.028 01/25/2006 . RCW 00 00 11 20 161 24 00 19.17 93.9 0.009 274.14 0.028 01/26/2006 RCW- 00 00 24 00 2400 117.62 0.009 343.39 . 0.028 01/27/2006 RCW .. 00 00 24 00 24.00 30.58 0.002 89.28 0.007 01/28/2006 RCW 00 24 00 24.00 30.58 0.002 89.28 0.007 01/29/2006 RCW 00 00 24 0 24.00 30.58 0 002 89.28 0.007 01/30/2016 RCW 00 : 00 24 0 3058 0.002 89.28 0.007 01/31/2006 RCW 00 : 0 25 152421 0.002 70.68 0.007
PCL 222 PCL-222 system injection start injection injection end timestarttime injection duration in 1 end time hours COPOLYMER PHOSPHATE
,___* total quantity mass balance calc: total quantity mass balance calc.
760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 02/01/2006 RCW 00 00 24 00 2400 28.23 0.002 82.41 0.007 02/02/2006 RCW 00 00 09 50 16 70 24 :100 17.83 20.97 0.002 61.22 0.007 02/03/2006 RCW 00 0 244 00 24.00 28.23 .0.002 82.41 0.006 02/04/2006 RCW 00 00 24 00 24.00 28.23 0.002 82.41 0.006 02/05/2006 RCW 00 00 24:00 24.00 28.23 0.002
- 82.41 0.006 02/06/2006 RCW 00 00 24 00 24.00 28.23 0.002 82.41 0.006 02/07/2006 RCW 00 00 09 : 30 9.50 23.28 0.005 67.96 0.013 02/08/2006 N/A N/A 02/09/2006 . -
02/10/2006 N/A 02/11/2006 N/A 02/12/2006 N/A 02/13/2006 N/A 02/14/2006 N/A 02/15/2006 N/A 02/16/2006 N/A- - 02/18/2006 N/A 02/19/2006 N/A N/A_____ 02/12/2006 N/A 02/21/2006 N/A 02/21/2006 N/A 02/23/2006 N/A 02/24/2006 N/A i 02/25/2006 N/A 02/26/2006 N/A 02/27/2006 N/A 02/28/2006 N/A 102/28/2006 N/A
PCL 222 injection injection injection, injection duration in PCL-222 system start time end time start time end time hours COPOLYMER PHOSPHATE total quantity mass balance calc. total quantity mass balance calc. 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 03/01/2006 N/A 03/02/2006 N/A 03/03/2006 N/A 03/04/2006 N/A 03/05/2006 N/A 03/06/2006 N/A 03/07/2006 N/A 03/08/2006 N/A 03/09/2006 N/A 03/10/2006 N/A 03/11/2006 N/A 03/12/2006 N/A 03/13/2006 N/A 03/14/2006 N/A 03/15/2006 N/A 03/16/2006 N/A 03/17/2006 N/A 03/18/2006 N/A 03/19/2006 N/A 03/20/2006 N/A 03/21/2006 N/A 03/22/2006 N/A 03/23/2006 N/A 03/24/2006 N/A 03/25/2006 N/A 03/26/2006 N/A 03/27/2006 N/A 03/28/2006 N/A 03/29/2006 N/A 03/30/2006 RCW 14 15 24 00 975 22.93 0.005 66.96 0.013 03/31/2006 RCW F00: 00 24 00 24.00 56.46 0.005 164.83 0.013
PCL 222 injection injection injection injection duration in PCL-222 system start time end time start time end time hours COPOLYMER PHOSPHATE total quantity mass balance calc. total quantity mass balance calc. 760 lbs/day 0-2 mg/L 2,280 lbs/day 0.2 mg/L 04/01/2006 RCW 00 00 24 00 24.00 56.45 0.005 164.83 0.013 04/02/2006 RCW 00 00 24 00' 24.001 56.45 0.005' 164.83 0:013 04/03/2006 RCW 00 00 10 20 15 35 24 00 18.75 44.11 0.005 128.77 0.013 04/04/2006 RCW 00 00 09 55 15 .05 24 00 18.83 '44.32 0.005 129.39 0.013 04/05/2006 RCW 0 0 08 45 13 45 24 00 19.00 44.69 0.005 130.49 0.013 04/06/2006 RCW 00 00 09 00 14 30 24 00 18.50 43.52 0.005 127.05 0.013 04/07/2006 RCW 00 100 24 00 24.00 56.46 0.005 164.83 0.013 04/08/2006 RCW 00 :100 24 00 24.00 . 56.46 0.005 164.83 0.013 04/09/2006 RCW 00 00 24 00 24.00 56.46 0.005 164.83 0.013 04/10/2006 . RW00 00 24 00 24.00 56.46 0.004 164.83 0.013 04/11/2006 RCW 00 09 15 14 55 24 :00 18.33 43.12 0.007 125.91 0.019 04/12/2006 RCW 00 00 08 40 13 25 24 :00 -19.25 45.28 0.008 132.2 0.023 04/13/2006 RCW 00 08 55 14 10 24 :00 18:75 44.11 0.008 12877 0.023 04/14/2006 RCW 00 00 24 00 24.00 56.46 0.008 16483 0023 04/15/2006 RCW 0 00 24 00 24.00 56.46 0.008 164.83 0.023 04/16/2006 RCW 00 00 24 -o00 24.00 56.46 0.008 164.83 0.023 04,17/2006 0RW 10 .30 1 4 :00 1. 43.52 0.008 127.05 0.023 04/18/2006 RCW 001 00 10 35 15 00 24 -:00 19.58 46.06 0.008 134.49 0.022 04/19/2006 RCW 001 00 .11 00 16 25 24 :100 18.58, 43.71 0.008 127.6 0.023 04/20/2006 RCW 00 : 10: 40 15 15 24 00 19.42 45.68 0.008 133.37 0.023 04/21/2006 RCW 00 :100 09 25 141 15 24 00: 19.17 45.09 0.008 131.65 0.023 04/22/2006 RCW 00 . 24 00 . 24.00 56.46 0.008 .164.83 0.022 04/23/2006 RCW 00 00 24 00 . . 24.00 56.46 0008 164.83 0.022 04/24/2006 RCW 00 00 10 25 15: 55 24 :00 18.50 43.52 0.008 127.05 0.022 04/25/2006 RCW 00 :00 08 50 13 30 24 :00 19.33 45.47 0.007 132.75 0.021 04/26/2006 . RCW 00 00 11 35 _ 11.58 27.24 0.007 79.53 0.021 04/27/2006 N/A .... 04/28/2006 N/A 04/29/2006 N/A 04/30/2006 N/A
PCL 222 injection injection injection injection duration in PCL-222 system start time end time start time end time hours COPOLYMER PHOSPHATE total quantity mass balance calc. total quantity mass balance calc.
" 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 06/01/2006 n/a 06/02/2006 n/a 06/03/2006 n/a 06/04/2006 n/a 06/05/2006 RCW 15 25 24 00 8.58 20.18 . 0.004 58.93 0.013 06/06/2006 RCW 00 00 10 30 16 10 24 00 18.33 43.12 0.004 125.88 0.013 06/07/2006 RCW 00 00 10 40 15 10 24 00 19.50 4587 0.004 133.92 0.013 06/08/2006 RCW 00 00 10 10 15 00 24 00 19.17 45.09 0.004 131.66 .0.013 06/09/2006 RCW 00 00 13 05 18 25 24 00 18.67 43.89 0.004 128.15 0.013 06/10/2006 n/a -_i.
06/11/2006 n/a 06/12/2006 RCW 00 00 10 30 15 10 24 00 19.33 45.47 0.004 132.75 0.013 06/13/2006 RCW' 00 00 11 55 16 35 24 00 19.33 45.49 0.004 132.82 0.013 06/14/2006 ERCWA & B 00 00 24 00 24.00 RCW 00 00 11 50 17 05 24 00 18.75 171.13 0.014 499.63 0.041 06/15/2006 ERCWA & B 00 00 24 00 ' 24.00 RCW 00 00 08 30 13 05 24 00 19.42 158.59 0.013 463.02 0.039 06/16/2006 ERcWA& B 00 00 .24 00 .24.00 RCW 00 00 12 35 17 150 24 :100 18.75 157.02 0.013 458.42 -0.038 06/17/2006 ERCWA& B& RCW 00 : 00 24 00 24.00 169.37 ° 0.013 494.48 0.038 06/18/2006 ERCWA & B & RCW 00 00 24 00 24.00 169.37 00.013 494.48 0.038 06/19/2006 ERCW A& B 00 00 24 00 24.00 RCW 00 00 09 40 15 05 24 00 . 18.58 156.64 0.013 457.32 0.038 06/20/2006 ERCW A& B 00 00 24 00 24.00 : RCW 00 00 09 35 14 05 24 00 19.50 158.78 0.013 463.57 0.038 06/21/2006 ERCW A& B 00 00 24 00 24.00 RCW 00 00 09 50 15 10 24 00 18.67 156.81 0.013 457.8 0.038 06/22/2006 ERCW A& B 00 00 24 00 24.00 RCW : 00 00 08 30 13 15 24 00 19.25 158.19 0.013 461.86 0.038 06/23/2006 ERCW A & B 00 00 24 00 24.00 RCW, 00 00 09 55 15 40 24 00 18.25 155.84 0.013 454.99 0.038 06/24/2006 ERCW A& BR&RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.038 06/25/2006 ERCW A & B & RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.038 06/26/2006 ERCW A & B 00 00 07 00 7.00 RCW 00:00 11745 162524:00 19.33 78.4 0.013 228.9 0.037 06/27/2006 RCW 00 00 10 10 15 00 24 00 19.17 45.09 0.004 131.66 0.0125 06/28/2006 RCW 00 00 09 45 15 15 24 00 18.50 43.52 0.004 127.05 0.013 06/29/2006 ERCW A& B 09 30 24 00 14.50 RCW 00 00 08 30 13 05 24 00 19.42 113.9 0.013 332.54 0.038 06/30/2006 ERCWA & B 00 00 24 00 24.00 RCW 00 0012 30 1 45 24 00 18.75 157.02 0.013 458.42 0.038
PCL 222 injection injection injection kinjection duration int PCL-222 system start time end time start time end time hours COPOLYMER PHOSPHATE total'quantity mass balance calc. total quantity mass balance calc.
-do- 4 - 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 07/01/2006 ERCWA& B& RCW 00 00 24 24.00 169.37 0.013 494.48 0.0375 07/02/2006 ERCW A& B & RCW 00 .00 24 00 24.00 169.37 0013 494.48 00375 07/03/2006 ERCWA & B 00 00 24 00 24.00 RCW 00 00 10 .35 151 15 24 00 19.33 158.38 0.013 462.41 0.038 07/04/2006 ERCW A& B 00 00 24 00 24.00 RCW 00 00 08 20. 13 :05 24 :00 19.25 158.19 0.013 461.86 0.038 07/05/2006 ERCWA & B oo7 7 00 "7.00 RCW 00 00 08 30 13 00 24:00 19.50 78.8 0.013 230.07 ,0,038 07/06/2006 RCW 00 00 09 05 14 45 24 00 18.33 43.12 0.004 125.89 0.013 07/07/2006 ERCWA& 8 16 35 24 00 7.42 RCW 00 00 09 10 14 25 24 00 18.75 79.01 0.013 . 230.69 0.038 07/08/2006 ERCW A& B & RCW 00 00 24 00 24.00 .169.37 0:013, 494.48 0.038 07/09/2006 ERCWA,& B& RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.038 07/10/2006 ERCWA& B 00 00 24 00 24.00
_RCW 00 00 09 30 14 30 24 00 19.00 157.61 0.013 460.14 0.039 07/11/2006 ERCWA& B& RCW 001:100 124 00 24.00 169.37 0.013 494.48 0.038 07/12/2006 ERCWA& B& RCW 001: 00 24 00 24.00 169.37 0.013 494.48 0.038 07/13/2006 ERCWA& B 001 :00 24 00 24.00 RCW 00 00 14 30 19 00 24 00 19.50 158.78 0.013 463.57 0.038 07/14/2006 ERCWA& B 00 00 24 00 24.00 RCW -00 00 11 45 16 55 24 00 T8.83 157.21 0.013 458.97 0.038 07/15/2006 ERCWA& B 00 00 24 00 24.00 RCW 00 00 10 35 151 05 24 :00 19.50 158.78 0.013 463.57 0.038 07/16/2006' ERCWA& B 00 100 24 00 24.00 RCW 00 100 09 :10 14 45 24 00 18.42 156.24 0.013 456.16 0.037 07/17/2006 ERCWA& B 00 00 24 00 24.00 RCW 00 00 11 05 16 00 24 00 19.08 157.8 , 0.014 460.71 0.041 07/18/2006 ERCWA& B 00 00 24 :00 24.00 RCW 00 00 16 00 21 15 24 00 18.75 157.02 0.013 458.42 0.039 07/19/2006 ERCWA& B 00 00 .24 00 24.00 RCW 00 00 10 05 14 55 241: 00 19.17 157.98 0.013 461.24 0.039 07/20/2006 ERCWA & B 00 100 24 00 24.00
,._ RCW 00 00 11 55 16 35 24 00 19.33 158.41 0.013 462.47 0.039 07/21/2006 ERCWA& B 00 00 24 00 ' 24.00 RCW 00 00 10 50 16 105 24 00 18.75 157.02 0.013 458.42 0.039 07/22/2006 ERCWA & B & RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.039 07/23/2006 ERCWA& B& RCW 00 00 24 00 24.00 169.37 0.013 .494.48 0.039 07/24/2006 ERCWA & B 00 24 00 24.00 RCW 00 00 09 05 J13 :45 24 00 19.33 158.38 0.013 462.4 " 0.038 07/25/2006 ERCW A & B 00 00 24 00 24.00 RCW . 00 00 09 20 14 20 24 00 19.00 157,58 0.013 460.14 0.038 07/26/2006 ERCW A & B 00 00 24 00 24.00 RCW 00 00 10 15 16 :-00 241:100 18.25 155.84 0.013 454.99 0.039 07/27/2006 ERCWA& B 00 00 24 00 24:00 RCW 00 00 10 40 15 50 24 00 18.83 157.23 0.013 459.04 0.039 07/28/2006 ERCWA& B 00 00 24 00 24.00 RCW 00 00 11 10 15 40 24 00 19.50 158.76 0.013 463.57 0.038 07/29/2006 ERCWA & B & RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.038 07/30/2006 ERCWA & B & RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.038 07/31/2006 ERCWA& B& RCW 00'00 2 . 24.00 169.37 0.013 494.48 0.038
PCL 222 injection injection injection injection duration in PCL-222 system start time end time start time endtime hours COPOLYMER PHOSPHATE total quantity mass balance calc. total quantity mass balance calc.. 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 08/01/2006 ERCW A & B 0 00 24 [002., RCW 00 00 13 00 17 30 24 00 19.50 .78 013 463.57 0.038 08/02/2006 ERCW A & B 00 00 24 00 24.00 RCW 00 00 11 00 16 20 24 00 18.67 156.81 0.013 457.8 0.038 08/03/2006 ERCW A& B 00 00 24 00 24.00 RCW 00 00 09 55 14 40 24 00 19.25 158.19 0.013 461.86, 0.039 08/04/2006 n/a 08/05/2006 ERCW A & B 00 00 24 00 24.00 RCW 00 00 08 35 13 30 24 00 19.08 157.79 0.013 460.69 0.038 08/06/2006 ERCW A &"3 & RCW 00 00 24 00 24.00 169.37 0.013 494:48 . 0.038 08/07/2006 ERCW A & B 00 00 07 20 7.33 RCW 00:00 10 45 16:05 24:00 18.67 78.4 0.013 228.9 0.038 08/08/2006 RCW 00 00 09 25 13 .55 24 00 19.50 45.87 0.004 133.92 0.013 08/09/2006 RCW. 00 00 24 00 24.00 56.46 0.004 164.83 0.013 08/10/2006 RCW 00 00 09 10 14 45 24 00 18.42 43.33 0.004 126.5 0.013 08/11/2006 RCW 00 00 24 00 24.00 56.46 0.004 164.83 0.01.3 08/12/2006 RCW 00 00 08 48 14 05 24 00 18.72 44.04 0.004 128.56 0.013 08/13/2006 RCW 00 :100 09 00 13 35 24 00 45.68 0.004 133.37 0.013 08/14/2006 RCW 00 00 24. 00 24.00 56.46 0.004 164.83
. . 0.012 08/15/2006 RCW 00 00 10 50 15 30 24 00 19.33 45.47 .0.004 132.76 0.012 08/16/2006 RCW 00 00 08 35 14 15 24 00 18.33 43.12 0.004 125.88
.. 0.013 08/17/2006 RCW 00 00 09 05 141 30 24 00 18.58 43.71 0.004 127.6 0.012 08/18/2006 ERCW A& B 10 45 24 00 13.25 RCW 00 00 10 45 161 00 24 00 18.75 106.44 0.013 310.77 0.037 08/19/2006 ERCWA & B 00: 00 24 00 24.00 RCOW 00 00 09 40 14 20 24 00 1933 158.41 0.013 462.47 0.037 08/20/2006 ERCW A & B & RCW 00 00 24 00 24.00 169.37 0.013 494.48 0.037 08/21/2006 ERCW A & B 00 00 24 00 24.00 RCW . 00 00 07 30 7.50 130.55 0,.013 381.16 0.037 08/22/2006 ERCW A& B 00 00 00 24.00 112.91 0.008 329.65 0.025 08/23/2006 ERCW A& B 00 00 24 00 24.00 112.91 0.008 329.65 0.025 08/24/2006 ERCWA & B 00 100 24 00 1 24.00 112.91 0.008 329.65 0.025 08/25/2006 ERCW A& B 00 00 24 00 24.00 RCW. 17 20 24 00 6.67 128.6 .0.013 . 375.46 0.037 08/26/2006" ERCW A& B 00 00 24 00 24.00 RCW 00 00 08 : 30 13 35 24 00 18.92 157.42 0.013 459.59 0.037 08/27/2006 ERCW A & B 00 00 24 00 24.00
- RCW 00:00 09:05 16:0524:00 17.00 152.9 0.013 446.4' 0.039 08/28/2006 ERCWA & B 00 24 00 24.00 RCW 00 00 09.: 14 0 24:00 19.33 158.38 0.013 462.4 .0037 08/29/2006
- ERCWA & B 00 00 24 00 24.00 RCW 00 00 08 00 13 40 241: 00 18.33 156.03 0.013 455.54 1 0.038 08/30/2006. ERCW A& B 00 00 24 00 24.00 ROW 00 00 11 10 15 55 24 00 19.25 158.19 0.013 461.86 " 0.038 08/31/2006 ERCW A& B 00 00 24 : 0 24.00
_RCW 00 00 11 25 16 00 24 :100 19.42 158.59 0.013 463.02 0.038
PCL 222 injection injection injection injection duration in PCL-222 system start time end time start time end time hours 'COPOLYMER PHOSPHATE
"__total quantity mass balance calc. total quantity mass balance calc.
760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 09/0.1/2006 ERCWA& B 00 00 24 00 24.00 112.91 0.009 329.65 0.025 09/02/2006 ERCWA& B 00 00 24 00 24.00 112.91 0.009 329.65 0.025 09/03/2006 ERCWA& B 00 100 24 100 24.00 112.91 0.009 329.65 0.025 09/04/2006 ERCWA& B 00 00 24: 00 24.00 112.91 .009 329.65 0.025 09/05/2006 ERCWA& B 00 00 24 00 24.00 112.91 0.008 329.65 0.024 09/06/2006 ERCWA& B 00 00 24 00 24.00 112.91 0.008 '329.65 0.024 09/07/2006 ERCW A& B 00 00 24 00 24.00 112.91 0.008 329.65 0.025 09/08/2006 ERCWA& B 00 00 24 00 24.00 .112.91 0.008 329.65 0.024 09/09/2006 ERCWA& B 00 00 24 : 00 24.00 112.91 0.008 329.65 0:024 09/10/2006 ERCWA & B 00 :.00 24 00 24.00 112.91 0.008 329.65 0.024 09/11/2006 ERCWA& B 00 :100 24 00 24.00 112.91 0.008 329.65 0.024 09/12/2006 ERCWA & B 00 : 00 24 00 24.00 112.91 0.008 329.65 0.024 09/13/2006 ERCWA& B 00 00 10 : 25 10.42 49.02 0.008 143.12 0.024 09/14/2006 n/a 09/15/2006 n/a 09/16/2006 n/a 09/17/2006 n/a 09/1.8/2006 ERCWA& B 141 20 24 00 9.67 45.49 0.008 132.82 0.025 09/19/2006 ERCWA& B 00 : 00 241 00 24.00 112.91 0.008 329.65 0.024 09/20/2006 ERCWA& B 00 100 241 00 24.00 112.91 0.008 329.65 0.025 09/21/2006 ERCWA& B 00 00 241: 00 24.00 112.91 0.008 329.65 0.025 09/22/2006 ERCWA& B 00 00 24 00 24.00 112.91 0.008 329.65 0.025 09/23/2006 ERCWA& B .00 00 24': 00 24.00 112.91 0.009 329.65 0.025 09/24/2006. ERCWA& B 00 00 24 00 24.00 112.91 0.009
- 329.65 0.025 09/25/2006 ERCWA& B 00 00 24 : 00 24.00 112.91 0.008 329.65 0.025 09/26/2006 ERCWA& B 00 :00 24 00 24.00 112.91 0.008 329.65 0.025 09/27/2006 ERCWA& B 00 : 00 24 00 24.00 .112.91 0.008 329.65 0.025 09/28/2006 ERCWA& B 00 00 24 .00 .24.00 112.91 .0.008 329.65 0.025 09/29/2006 ERCWA& B 00 : 00 24 00 24.00 112.91 0.008 329.65 0.025 09/30/2006 ERCWA& B 00 : 00 24 : 00 24.00 1.12.91 0.008 329.65 0.025
PCL 222 injection injection injection injection duration in PCL-222 system start time end time start time end time hours COPOLYMER PHOSPHATE _faotal quantity "mass balance calc. total quantity mass balance calc. _ 760 lbs/day 0.2 mg/L 2,280 lbs/day 0.2 mg/L 10/01/2006 ERCWA & B 00 00 24 00 *24.00 112,91 0.008 329:65 0.025 10/02/2006 ERCW A& B 00 00 07 52 7.87 37,03 0.008 108.1 0.025 10/03/2006 n/a 10/04/2006 n a 10/05/2006 n/a 10/06/2006 n/a 10/07/2006. n/a 10/08/2006 n/a 10/09/2006 n/a 10/10/2006 n/a 10/11/2006 n/a 10/12/2006 n/a 10/13/2006 n/ai 10/14/2006 n/a 10/15/2006 n/a 10/16/2006 n/a 10/17/2006 n/a 10/18/2006 n/a 10/19/2006 n/a " 10/20/2006 n/a ._ _ 10/21/2006 n/a 10/22/2006 n/a 10/23/2006 n/a 10/24/2006 n/a 10/25/2006 n/a 10/26/2006 n/a 10/27/2006 n/a 10/28/2006 n/a 10/29/2006 n/a 10/30/2006 n/a 10/31/2006 n/a
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report Towerbrom 960 Duration in Quantity in Pounds Hours Analytical Results (lbs/day) (hrs/day) mass balance calculation (mg/L)
#of Daysin Month Maximum Average Limit Maximum Maximum Average Limit samples Service (based on days in 4.
service) 4" January 150 77 1425 .5.25 0.024 0.007 0.10 25 13 February 510 335 1425 24.00 0.040 <0.019 0.10 34 22 March 620 529 1425 24.00 0.029 0.018 0.10 30 30 April 610 212 1425 24.00 0.049 0.026 0.10 20 20 May 580 308 1425 24.00 0.028 0.014 0.10 28 28 June 380 148 1425 16.75. 0.030 0.020 0.10 26 26 July 520 199 1425 13.58 0.028 0.020 0.10 .22 22 August 560 238 1425 21.75 0.034 0.018 0.10 28 26 September 280 184 1425 5.03 0.033 0.020 0.10 25 25 October 530 236 1425 24.00 0.022 0.012 0.10 28 26. November 610 371 1425 24.00 0.038 0.014 0.10 30 : 30 December 480 148 1425 24.00 0.032 0.017 0.10 18 18 Jan. - Dec. 314 286 Per the B/CTP approval: "Whole Effluent toxicity testing (biomonitoring) of Outfall 101 and Outall 110 shall be undertaken once per year when oxidizing biocides are being- used.." Outfall 101; Toxicity was sampled November 26-December 1, 2006. Test Results:," Pimephales promelas: IC25 >100% Ceriodaphniadubia: IC25 > 100% Outfall 110: There has been no discharge from Outfall 110 January - December 2006.
Towerbrom 960 system injection _injection, duration in hours 1,425 total quanti Ibs/day mass balance caic. 0.10 mg/L Additional 0.10Results mg/L January 2006 01/01/2006 N/A - 0.0000 01/02/2006 N/A 0.0000 01/03/2006 ERCWA & B 11 15 15 45, 4.501
. RCW 12: 45 16 45 4.00 150 0.0243 01/04/2006 ERCWA & B 09 45 13 50 4.08 RCW 11 25 15 300 4.08 ;140 0.0200 01/05/2006 N/A 0.0001 01/06/2006 N/A 0.0001 01/07/2006 N/A 0.0001 01/08/2006 N/A . 0.0001 01/09/2006 ERCWA&B 10 :100 14 08 4.13 100 0.0151 01/10/2006 N/A 0.0000 01/11/2006 N/A. 0.00.00 01/12/2006 RCW 09 :30 13 :22 3.87 30 0.0087 01/13/2006 ERCWA&B, 10 00 14 : 00 4.00 RCW 11 00 14:25 3.42 100 0.0181 .01/14/2006 N/A 0.0000 01/15/2006 N/A 0.0000 01/16/2006 N/A 0.0001 . .01/17/2006 N/A . 0.0001 01/18/2006 RCW 10 55 15 05 4.17 20 0.0111 01/19/2006 RCW 11 15 15 30 4.25 20 0.0056 01/20/2006 ERCWA & B 081: 35 13 50 5.25 80 0.0068 01/21/2006 ERCWA & B 09 37 13 51 4.23 80 0.0124 01/22/2006, N/A '_ _*_'
01/23/2006 ERCWA&B 12 :10 16 15 4.08 RCW 13 -.35 17 45 4.17 100 0.0144 01/24/2006 ERcWA & B 10 : 30 14 45 4.25 RCW 11 : 35 161: 05 4.50 120 0.0123 : 01/25/2006 . RCW 11 55 15 55 4.00 30 0.0084 01/26/2006 N/A ._ _ __
*01/27/2006, N/A 01/28/2006 N/A _ _
01/29/2006. N/A 01/30/2006 N/A 01?31/2006 RCW 11 g15 15i:15 4.00 - 30 0.0096
Towerbrom 960 system injection injection duration in hours total quantity mass balance calc. Additional Results 1 1,425 lbs/day 0.10 mg/L 0.10 mg/L 02/01/2006 ERCWA & B 13 :130 171,: 30 4.00 80 0.0061 02/02/2006 RCW 11 00 15 50 4.83 30 0.0084
.02/03/2006 ERCWA & B 10 15 14 20 4.08 100 . 0.0103 02/04/2006 N/A 02/05/2006 N/A 02/06/2006 'N/A 02/07/2006 ERCWA &,B 08 40 24:00 15.33 RCW .10 45 24 :00 13.25 510 0.0176 02/08/2006 ERCW A & B & RCW 00 00 24 :100 24.00 460 0.0111 02/09/2006 ERCWA & B &RCW 00 00 24 00 24.00 410 0.0130 02/10/2006 ERCWA & B & RCW .00 00 24 00 24.00 400 0.0152 -
02/11/2006 ERCWA & B &RCW 00 00 24 00 24.00 380 0.0134 02/12/2006 ERCWA & B.& RCW 00 00 24 00 . 24.00 400 0.0181 02/13/2006 ERCWA & B & RCW 00 00 24 00 24.00 400 0.0133 02/14/2006 ERCWA.& B.& RCW 00 00 24 00 24.00 340 0.01.83 02/15/2006 ERCWA & B & RCW 00 00 24 00 24.00 380 0.0165 02/16/2006 ERCWA & B & RCW 00 00 24 00 24.00 390 0.0206 02/17/2006 ERCWA & B & RCW 00 00 24 00 24.00 380 0.0072 02/18/2006 ERCWA &'B & RCW 00 00 24 00 24.00 300 0.0128 02/19/2006 ERCWA& B 00 " 00 11 30 11.50 RCW 00 00 13 50 13.83 70 <0.0057 0.02 0.03 0.03 0.03 02/20/2006 N/A . 0.03 0.03 0.03 0.03 02/21/2006 N/A 0.03 0.03 0.03 0.04 02/22/2006 N/A 02/23/2006 ERCWA & B .16 45 24 00 1 T25 RCW 16 :10 24 : 00
- 7.83 290 0.0130 02/24/2006 ERCWA & B & RCW 00 : 00 24 :00 24.00 410 0.0162 02/25/2006 Et3CWA & B & RCW 00 :00 24 " 00 24.00 410 '0.0087 02/26/2006 ERCWA & B & RCW 00 : 00 24 : 00 24.00 340 0.0117 02/27/2006 ERCWA & B & RCW 00 : 00 24 : 00 24,00 430 0.01,46 02/28/2006 *ERCW A & B & RCW, 00 1 00 24 : 00 24.00 . 4501. 0.01901
Towerbrom 960 system Injec ton injection duration in hours total quanti massbalance caJc. Additional Result*
.1,425 lbs/day 0.10 mg/L 0.10 mg/L 03/01/2006 ERCWA&B&ROW 00 00 24 00 24.00 460 0.0183 0310212006 ERCWA & B & RCW 00 00 24 00 2400 440o.. 0.0195 03/03/2006 ERCW A & B & RCW 00 00 24: 00 24.00 440 0.0188 03/04/2006 ERCWA & B & RCW 00 00 24 00 24.00 440 0.0196 03/05/2006 ERCWA & B & RCW 00 .00 24 00 24.00 440 " 0.0146 03/06/2006 ERCWA & B & RCW 00 00 24 00 24.00 450 0.0136 03/0712006 ERCW A & B & RCW 00 00 24 00 24.00 450 0.0073 03/08/2006 ERCWA & B & RCW 00 00 24001 24.00 510 0.0086 03/0912006 ERCWA & B & RCW 00 00 24 00l 24.00 ý520 0.0116 03/10/2006 ERCWA & B'& RCW 00 00 24 001 24.00 520 0.0129 03/11/2006 ERCWA & B & RCW 00 00 24 00 24.00 520 0.0145 03/12/2006 ERCWA & B & RCW 00 00 24 00 24.00 520 0.0139 03/13/2006 ERCW A & B 00 00 18 58 18.97
- RCW 00 00 24 00 24.00 520 0.0293 03/14/2006 ERCWA & B 001:104 24 00 23.93 RCW 00 00 24 00 24.00 530.. 0.0095 03/15/2006 ERCWA & B & RCW 00 00 24 :100 24.00 620 0.0231 03/1612006 ERCWA & B & RCW 00 00 24 :.00 2400 610 0.0285 03/17/2006. ERCWA & B & RCW 00 00 241:100 24.00 600 0.0265 03/18/2006 ERCWA & B & RCW 00 00 24 00 24.00 600 0.0152 03/19/2006 ERCWA & B & RCW 00 00 24 00 24.00 610 0.0159 03/20/2006 ERCWA & B & RCW 00 00 24 00 . 24.00 610 0.0252 03/21/2006 ERCWA & B & RCW 00 00 24 00 24.00 610 0.0269 03/22/2006. ERCWA & B & RCW 00i 00 24 00 24.00 *.610 0.0279 03/23/2006 ERCWA & B & RCW 00 00 24 00 24.00 590 0.0113 03/24/2006 ERCW A & B,& RCW 00 00 24 00 24.00 590 0.0186 03/25/2006 ERCW A & B & RCW 00 00 24 00 24.00 590 0.0199 --
03/26/2006 ERCW A & B & RCW 00 00 24 :00 24.00 590 0.0159 _ " 03/27/2006 ERCWA & B & RCW 00 :00 24 00 24.00 590 0.0113 03/2812006 ERCWA & B & RCW 00 00 24 00 24.00 580 0.0135 03/29/2006 ERCW A & B & RCW 0 00 .
- 24.00 " 600 0.0169 __
03/30/2006 ERCWA & B 00 00 12 56 12.93 RCW 00 00 13 34 13.57 120 0.0160 7- 03/31/2006 n/a
Towerbrom 960 system injection injection duration in hours total quantity mass balance calc. Additional Result 0.10 mg/LI .__ 0. 10 mgIL 04 /01 /2006 n/a0/120 /'1,425 lbs/day 04/0212006 n/a
'04/03/2006 ERCWA&B 11 15 15 :45 4.50 RCW 10 30 15:25 4.92 130 0.0167 04/04/2006 ERCWA & B 09 30 13 30 4.00 ROW 10 :50 15 00. 4.17 140 0.0144 04/05/2006 ERCWA&B 10 :10 14 15 4.08 RCW 09 10 13 35 4.42 110 0.0173 04/06/2006 ERCWA&B 12 15 16 115 4.00 RCW 10 35 14 20 3.75 160 0.0192 04/07/2006 n/a 04/08/2006 n/a 04/09/2006 n/a 04/10/2006 n/a 04/11/2006 ERCWA&B 13 50 17 '50 4.00 RCW 10 10 14 50 4.67 130 0.0287 04/12/2006 RCW 09 00 13 20 4.33 30 0.0148 04/13/2006 ERCWA&B 11 15 15 15 4.00 RCW 09 : 45 14 00 4.25 130 '0.0385 04/14/2006 ERCWA & B o08 : 50 12 50 4.00 120 0.0225 04/15/2006 n/a 04/16/2006 n/a 04/17/2006 ERCWA &B 10 00 14 00 4.00 RCW 11 :120 15 50 4.50 150 0.0309 04/18/2006 ERCWA & B 12 :105 16 05 4.00 RCW 10 :50 14 50 4.00 150 0.0494 04/19/2006 ERCWA.& B 12 :15 14 :,20 2.08 RCW 12 : 15 16 :15 4.00 150 0.0399 04/20/2006 .ERCWA&B 10.: 10 14 :12 4.03 RCW 11 00 15 00 4.00 150 . 00336 04/21/2006 RCW 09
- 40 14 00 4.33 30 0.0135 04/22/2006 n/a 04/23/2006 n/a 04/24/2006 ERCWA & B 14 :100 18 00 4.00 RCW 11 : 30 15 :140 4.17 150 0.0389 04/25/2006 RCW 09 : 05 13 15 4.17 30 0.0123 04/2612006 ERCWA&B . 09 :155 15 051 5.17 RCW . 12 .145 16 50 4.08 150 0.0314 04127/2006 ERCWA & B 16 : 35 24 00 7.42 RCW 12 :135 24 00 11.42 500 0.0264 04/28/2006 ERCWA &B & RCW 00 : 00 24 :00 24.00 610 0.0276 04/29/2006 ERCWA & B & RCW 00 : 00 24 00 24.00 610 0.0261 04/30/2006 ERCWA & B & RCW 00 :100 TI 241: 00 24.00 610 0.0229
Towerbrom 960 system injection injection duration in hours total quantity mass balance calc. Additional Results_ 1,425 lbs/day 0.10 mg/L 0.10 mg/L . 05/01/2006 ERCWA & B& RCW 00 00 24 00 24.00 580 0.0239 05/02/2006 ERCWA & B 00 00 14 00 14.00 RCW 00 :100 13 30 13.50 30 0.0154
" 05/03/2006 n/a 05/04/2006 ERCWA&B 13 :.15 24 :100 10.75 RCW 15 : 00 24 :100 9.00 520 0.0278 05/05/2006 ERCWA & B & RCW 00 : 00 24 :100 24.00 560 0.0225 05/06/2006 ERCWA & B & RCW 00 : 00 24 00 24.00 560 0.0200 05/07/2006 EROW.A & B & RCW 00
- 00 24 00 24.00 560 0.0220 05/08/2006 ERCW'A & B & RCW 00 00 24 " 00 24.00 460 0.0218 05/09/2006 ERCWA & B & RCW 00): 00 24 : 00 , 24.00 460 0.0214 05/10/2006 ERCWA & B & RCW 001: 00 24 : 00 24.00 560 0.0261 05/11/2006 ERCWA & B & RCW 00 :100 24
- 00 24.00 560 0.0162 05/12/2006 ERCWA & B & RCW 00 00 24 " 00 24.00 560 0.0196 05/13/2006 ERCWA&B 00 00 19 32 19.53 RCW 00 00 24 :,00 24.00 270 0.0135 05/14/2006 RCW 00 00 24 : 00 24.00 110 0.0042 05/15/2006 RCW 00 00 24 00 24.00 110 0.0048 05/16/2006 RCW 00 :100 24 00 24.00 110 0.0041 05/17/2006 RCW 00 00 24 00 24.00 140 0.0041 05/18/2006 RCW 00 00 24 00 24.00 120 0.0041 05/19/2006 ERCWA&B 13 15 24 00 10.75 RCW 00 00 24 00 24.00 350 0.0149 .
- 05/20/2006 ERCWA & B 00 00 13 30 13.50 RCW 00 00 24 00 24.00 320 0.0171 05/21/2006 RCW 00 00 24j: 00 24.00 160 0.0115 05/22/2006 RCW 00 00 24 :100 24.00 160 0.0072 05/23/2006 ROW 00 : 00 24 :100 24.00 180 0.0093 05/24/2006 ERCWA & B 14 : 30 24 : 00 9.50 RCW 00 ' 00 24 00 24.00 420 0.0180 05/25/2006 ERCWA & B 00 : 00 14 30 14.50 RCW .001: 00 24 00 24.00 220 0.0129 05/26/2006 RCW 00:00 24 00 24.00 160 0.0041 05/27/2006 RCW 00 00 24 00 24.00 160 0.0052 05/28/2006 RCW 00 : 00 16 : 40 16.67 .90 0.0052 05/29/2006 n/a 05/30/2006 n/a 05/31/2006 ERCWA & B 10 Z40 16 : 05 5.42 120 .0.0125
I-.. - ý... - Towerbrom 960 system injection injection duration in hourý total quantity mass balance calc. Additional ResultE -
*__ 1,425 lbs/day 0,10 mg/L 0.10 mg/L ,
0610112006 ERCW A & B 10 50 14 50 4.00 120 0.0129 06102/2006 ERCWA.& B 15 40 20 30 4.83 RCWW 12 55 24 00 11.08 220 0.0197 06/03/2006 ERCWA &'B 09 50 14 : 20 4.50 . RCW 00 00 14 35 14.58 180 0.0181 06/04/2006 ERCWA & B 09 : 20 14
- 01 4.68 120 0.0122 06/05/2006 ERCWA & B 09 : 45 13 : 45 4.00 RCW 10o 35 15 :15 4.67 170 0.0238 06/06/2006 ERCWA & B 09 :50 13 " 55 4.08 RCW 11 : 25 16 : 00 4.58 180 0.0255 06/07/2006 ERCW.A & B 09 40 13 : 45 4.08 RCW 11 00 15 00 6 4.00 170 0.0259 06/08/2006 ERCWA&,B 09 10 14 " 20 5.17
.RCW 10 : 35 14 : 50 4.25 170 0.0276 06/09/2006 ERCWA&B 11 : 35 16 " 45 5.17 RCW 14 : 00 18 " 15 4.25 15.0 0.0254 ,06/10/2006 n/a" 06/11/2006 n/a -FF 06/12/2006 ERCWA & B 09 :20 13 :25 4.08 RCW 10 55 15 : 00 4.08 150 0.0239 06/13/2006 ERCWA & B 10 55 15
- 05 , 4.17
_RCW 12 : 20 16
- 25 4.08 150 0.0235 06/14/2006 ERCWA & B 10 *40 14 : 55 4.25 RCW - 12 : 55 16
- 55 4.00 150 , 0.0204 06/15/2006 RCW 08 . 50 12 55 4.08 40 . 0.0112 06/16/2006 ERCWA&B 12 00 16 : 10 4.17 RCW 13 40 17 40 4.00 150 0.0238
.06/17/2006 ERCWA & B 08 55 12 :55 4.00 110 0.0105
. _06/18/2006 n/a 06/19/2006 ERCWA & B 09 15 14
- 00 4.75 ____
RCW 10 10 14 : 55 4.75 150 0.0284 06/2012006 ERCWA,& B. 09
- 05 14 : 20 5.251 RCW 09 55 13
- 55 4.00 150 0.0270 06/21/2b06 ERCWA & B 10 00 14
- 00 4.00 RCW 10 55 15 " 00 4.08 , 150 0.0244 06/22/2006 RCW 08:55 13 05 4.17 . 40 0.0166 06/23/2006 ERCWA & B 10
- 00 14
- 20 4.33 RCW . 09 00 15 : 30 6.50 150 0.0300 06/24/2006 ERCWA&B 09B:00 12 :42 3.7 110 0.0174 06T2-512006. n/a 06/26/2006 RCW 12 09 16 : 15 ' 4.10 40 0.0178 06/27/2006 ERCWA & B 16 40 24 1 00 7.33 RCW 09 20 14
- 50 5.50 380 0.0175 06/28/2006 ERCWA &B 00 00 16 : 45 16.75 RCW 10 45 15
- 05 4.33 240 0.0202 06/29/2006 RCW 08 : 50 12
- 55 4.08 50 0.0077 06/30/2006 ERCWA& B 11. : 10 16 : 15 5.08 RCW 13: 30 17 " 35 4.08 170 0.0184
Towerbrom 960 system injection injection duration in hours totalquantity mass balance calc.I Additional Result, _ .. 1,425 lbs/day 0. 10 mg/L 0.10 mg/L 07/01/2006 ERCWA&B 10 55 14 55 4.00 140 0.0085 07/02/2006 'n/,a 07/03/2006 ERCWA & B 12 25 16 :25 4.00j RCW 11 10 15 :110 4.00 190 0.0165 07/04/2006 RCW 08 55 12 :55 4.00 50 0.0055 07/05/2006 RCW 08 : 50 12 :50 ' . 4.00 60 0.0111 07/06/2006 ERCWA &.B 13 " 30 24 :00 10.50 RCW 10 15 14 : 35 4.33 520 0.0260 07/07/2006 ERCWA & B 00 00 13 35 13.58 RCW 10 15 14.: 15 4.00 120 0.0283 07/08/2006 n/a 07/09/2006 n/a 07/10/2006 ERCWA & B 08-: 55 131: 05 4.17 RCW 09 55 141: 05 4.17 200 0.0174 ,'. , 07/11/2006 n/a 07/12/2006 n/a, _ 07/13/2006 ERCWA & B 15 05 19 :105 . 4.00 RCW 15 55 18 55 3.00 180 0.0194 07/14/2006 ERCWA & B 10 35 16 00 5.42 RCW .. 12 45 16 45 4.00 180 '0.0224 07/15/2006 ERCWA & B 10 05 14 45 4.67 RCW .10 50 14 55 4.08 190 0.0223 07/1612006 ERCWA&B 11 10 15 15 4.08 RCW 09 55 14 35 .4.67 200 0.0214 07/17/2006 ERCWA & B 09 :25 13 35 4.17 RCWO 11 : 30 15 50 4.33 180 0.0188 07/18/2006 ERCWA & B 14 : 55 201: 15 5.33 RCW 16 :155 211: 05 4.17 200 0.0203 07/19/2006 fERCWA & B 09 :.130 141: 30 5.00 RCW 10 :135 14 :. 40 4.08 200 0.0241 "07/20/2006 ERCWA & B 10 : 35 141: 35 4.00 RCW . 12:120 161:125 4.08 230 0.0247 07/21/2006 ERCWA&B 10 : 15 14 1 35 4.33 RCW 11 55 151:155 4.00 260 0.0260 07/22/2006 n/a 07/23/2006 n/a _ 07/24/2006 ERCWA&B .. 11
- 00 15 : 00 . 4.00 _
RCW 09
- 25 13 : 35 4.17 230 0.0207 .
07/25/2006 ERCWA & B 10 : 50 14 :,55 4.08 RCW 101: 00 14 :08 4.13 220 0.0273 07/26/2006 ERCWA & B 10:00 14 :05 4.08
*RCW 10 40 14 '50 4.17 220 0.0248 07/27/2006 ERCWA&B 10 05 14 :08 4.05 RCW 11 40 15 :.40 4.00 220 0.0171 07/28/2006 ERCWA & B 10 55* 14 1 55 4.00 RCW ' 11 25 15 :130 4.08 210 0.0167 .
07/29/2006 n/a ;. 07/30/2006 n/a 07/31/2006 ERCWA&B 11 45 15 45 4.00 170 0.0101 4.0!7rOOf
Towerbrom 960 system injection injection duration in hour 1,425 total quanti mass balance calc. Additionai lbs/day 0.10 mg/L 0.10Result mgIL 08/01/2006 ERCWA & B 09 50 14 30 4.67
- RCW 13 20 17:20 4.00 240 0.0227 ;
08/02/2006 RCW 12 05 16 10 4.08 70 0.0094 " ,_. 08/03/2006 ERCWA &B 13 35 17 35 ,4.00 RCW 10 10 14 :130 4.33 260 0.0241 08/04/2006 ERCWA &B 11: 05 1.5 :15 4.17 RCW 12 20 16 55 4.58 270 0.0241 08/05/2006 RCW 09 " 00 13 25 4.42 90 0.0104 08/06/2006 n/a _ 08/07/2006 RCW 11 10 15 55 4.75 100 0.0103 08/08/2006 ERCWA &B 21 30 24 00 2.50 0.0091 RCW 09 :45 13 : 45 4.00 420 0.0087 08/09/2006 ERCWA &B 00 00 21 : 45 21.75 300 0.0072 08/10/2006 RCW 10 20 141: 35 4.25 120 0.0171 08/11/2006 n/a 08/12/2006 RCW 09 :145 131: 58 4.22 120 0.0084 08113/2006 ERCWA &B 13 50 17 : 50 4.00 0 /RCW 09 15 13 : 35 4.33 300 0.0221 0811412006_ n/a 08/15/2006 RCW i1 :15 15 : 20 4.08 120 0.0162 08/16/2006 ERCWA & B 13 31 24
- 00 10.48 RCW 09 35 14 05 4.50 560 0.0255 08/17/2006 ERCWA & B 00 00. 13 40 13.67 RCOW 09 :125 14 17 4.87 300 0.0337 08/18/2006 ERCWA&B 10 :130 14 40 4.17
_ RCW 11 45. 15 50 4.08 250 0.0329 08/19/2006 ERCOWA & B 09 20 131: 20 4.:00 RCW 10 15 14 15 4.00 280 0.0323 08/20/2006. n/a 08/21/2006 RCW 10 10 14 10 4.00 120 0'.0159
ý08/22/2006 ERCWA & B 09 50 13 50 4.00 180 0.0106 08/23/2006 n/a 08/24/2006 ERCWA & B 10 25 151: 24 4.98 0.0098 RCW 17: 06 24: 00 6.90 300 0.0071 08/25/2006 ERCWA & B 10 45 121: 45 2.00 RCW 00 00 17 10 17.17 290 0.0167 08126/2006 ERCWA & B 07
- 50 11 50 4.00 RCW 09 " 30 13 30 4.00 240 0.0144 08/27/2006 ERCWA & B 08 " 50 12 :100 3.17 RCW 09 : 55 13 :150 3.92 270 0.0262 08/28/2006 ERCWA&B 09 00 13 20 4.33 RCW 09:40 13 50 4.17 210 0.0219 08/29/2006 ERCWA & B 11 : 00 15 00 4.00 RCW 09 15 13 27 4.20 260 0.0207 08/30/2006 ERCWA&B 10 : 50 14 : 50 4.00 RCW 11 :40 15 45 4.08 270 0.0140 08/31/2006 ERCW A&B 08 :10 12 : 35. 4.42 RCW 11 :50 15 : 50 4.00 260 0.0235
- Towerbrom 960 system injection injection duration in hours total quantity mass blalance calc. Additional Resultý 1,425 lbs/day 0.10 mg/L 0.10 mg/L 09/01/2006 ERCWA&B 11 05 15 05 4.00 _
RCW 12 15 16 15 4.00 240 0.0210 09/02/2006 n/a 09/03/2006 n/a 09/04/2006 n/a ___" 09/05/2006 ERCWA&B 11 10 15 11 4.02 RCW 12 15 16 16 402 280 0.0241 09/06/2006 ERCWA & B 10 35 14 36 4.02 RCW 12 10 16 11 4.02 280 0.0276 09/07/2006 ERCWA & B 10 40 14 41 4.02 RCW 11 50 15 51 4.02 200 0.0215 09/08/2006 ERCWA & B 10 40 14 41 4.02 RCW 12 15 16 1:25 4.17 190 0.0218 09/09/2006 ERCWA & B 08 "55 13 :100 4.08
*RCW 09 : 20 13
- 25 4.08 190 0,0178 09/10/2006 n/a 09/11/2006 ERCWA & B 09
- 45 13 :146 4.02 RCW 10 :35 14 45 4.17 220 0.0165 09/12/2006 RCW 11 00 15 01 4.02 100 0.0188 09/13/2006 . RCW 09 35 13 " 36 4.02 100 0.0146 09/14/2006 RCW 09 50 13 " 51 4.02 100 0.0136 09/15/2006 RCW 09 10 13 23 4.22 100 0.0188 09/16/2006 ERCWA & B 09 05 13 05 4.00 150 0.0072 09/17/2006 ERCWA & B 10 :100 14 00, 4.00 200 0.0182 09/18/2006 ERCWA&B 10 00 14 10 4.17 :200 0.0109 09/19/2006 ERCWA & B 10 25 14 26 4.02 RCW 08: 55 12 56 4.02 270 0.0249 09/20/2006 ERCWA & B 09 40 13 41 4.02 RCW 10 25 14 32 4.12 250 0.0327 09/21/2006 ERCWA & B 10 10 14: 17 4.12 RCW 11.1:50 15 :53 4.05 240 0.0241 "09/2212006 ERCWA & B 09 40 13 :58 4.30 RcW 11 10 15 20 4.17 250 0.0312 09/23/2006 RCW 08 30 1'2 30 . 4.00 70 0.0309 09/24/2006 n/a _________
09/25/2006 ERCWA & B 09 05 13 20 4.25 RCW 10 : 10 14 30 4.33 190 0.0201' 09/26/2006 ERCWA & B 101:120 14 21 4.02 RCW 11 55 15 56 4.02 210 0.0246 09/27/2006 ERCWA & B 07 35 12 37 5.03 RCW 08 . 49 13 51 5.03 160 0.0203 09/28/2006 . RCW: 10 :15 14 20 4.08 50 0.0109 09/29/2006 ERCWA & B : 11 : 05 15 06 4.02 " RCW 11 ' 50 16 21 4.52 190 0.0206
.09/30/2006 ERCWA&B 09 : 55 13 :155 4.00 .180 0.0114
Towerbrom 960 system injection injection duration in hour*1total quantity mass balance calc. Additional Resultm/ _________1,425 lbs/day 0.10 mg/L 0.10 mg/L 10/01/2006 n/a 10/02/2006 RCW 11 35 15 45 4.17 40 0.0062 10/03/2006 ERCWA & B 14 30 24 00 9.50 0.0111 RCW 10 20 15 11 4.85 480 0.0050 10/04/2006. ERCWA & B 00 00 15 02 15.03 RCW 10 30,14 :135 4.08 270 0.0158 10/05/2006 RCW 11 :130 16 26 4.93 50 0.0093 10/06/2006 ERCWA & B 09 50 13 55 4.08 RCW 11 35 15 45 4.17 230 0.0179 10/07/2006 n/a 10/08/2006.. ERCWA&B 09:50_13 50 4.00 180 0.0108 10/09/2006 RCW 08 :150 12 : 50 4.00 :50 0.0094 10/10/2006 n/a 10/11/2006 ERCWA&B 11 :10 15 :.11 4.02 _ RCW 11 :52 15 53 4.02 .210 0.0199 10/12/2006 ERCWA & B 14 :00 24 00 10.00 RCW 111: 10 15 : 20 4.17 490 0.0199 10/13/2006 ERCWA & B 00 :100 24 : 00 24.00 _RCW 11 :145 16 : 14 4.48 450 0.0178 10/14/2006 ERCWA & B 00 : 00 24: 00 24.00 500 0.0089 10/15/2006 ERCWA & B 00 : 00124 : 00 24.00 RCW 11 40 15 : 40 4.00 500 0.0191 10/16/2006 ERCWA & B 00 00 24 : 00 . 24.00 RCW 12 05 16 :12 4.12 530 0.0188 10/17/2.006 ERCWA & B 00 0008 : 52 8.87 0.0212 RCW 10 32 14 : 32 4.00 180 0.0105 10/18/2006 RCW 10 35 14 :35 4.00 40 0.0105 10/19/2006 ERCWA&B .11 :40 24 00 12.33 250 0.0101 10/20/2006 ERCWA & B 00 00 16 00 16.00 RCW, 11 : 15 15 15 -4.00 .220 0.0223 10/21/2006 n/a 10/22/2006 RCW 09 00 13 00 4.00 40 0.0105 10/23/2006 RCW 10 35 14 35 4.00 40 0.0084 10/24/2006 . . RCW 091: 53 13 53 4.00 40 0.0099 10/25/2006 RCW 09 55 14 00 .4.08 40 0.0084 10/26/2006 ERCWA & B 08 25 24 00 15.58 510 0.0058 10/27/2006 ERCWA & B 00: 00 24 00 24.00 RCW .11.: 15 14 35 3.33 .480 0.0160 10/28/2006 ERCWA & B 00 : 00 14 00 , 14.00 110 0.0068 10/29/2006 RCW 08 35' 12 45 4.17 40 0.0115 10/30/2006 ERCWA & B 09 . 50 14 30 4.67 160 0.0068 10/31/2006 n/a ..
LJy.
- .. - ....JI -
Towerbrom 960 system injection injection duration in hours total quanti mass balance calc. Additional Results
. 1,425 lbs/day . 0.10 mg/L 0.10 mg/L 11/01/2006 ERCWA & B 08 25 12 25 4.00 180 0.0066 11/02/2006 ERCWA&B . 09 [35 13 35 4.00 200 0.0134 11/03/2006 ,, RCW 11 *15 24 *00 12.75 110 0.0039 11.104/2006 ERCWA & B 10 10 14 10 4.00 RCW 00 00 24 00 24.00 300 0.0127 _." "
11/05/2006 RCW 00 00 24 00 24.00 120 " 0.0039 11/06/2006 RCW 00 00 24 00 24.00 160 0.0041 11/07/2006 RCW 00 00 24 00 24.00 170 0.0093. 11/08/2006 RCW 00 00 24 00 24.00 160 0.0103 11/09/2006 ERCWA&B 18 55 24 00 .5.08 RCW 00 00 24 00 24.00 : ,20 0.0145 11/10/2006 ERCWA & B & RCW 00 00 24 00 24.00 600 0.0287 11/11/2006 ERCWA&B 00 : 00 15 :115 15.25 RCW 00 00 24 00 24.00 380 0.0090 11/12/2006 RCW 00 00 24 00 24.00 160 0.0055 11/13/2006 RCW 00 :00 24 00 24.00 .160 0.0068 11/14/2006 RCW 00 :00 24 00 24.00 140 0.0143i 11/15/2006 RCW 00 00 24 00 -24.00 150 0.0068 11/16/2006 ERCWA &B 14 : 00 24 : 00 10.00 RCW 00 :00 241: 00 24.00. 480 0.0267 11/17/2006 ERCWA & B & RCW 00 00 24 00 24.00 610 0.0222 11/18/2006 ERCWA & B & RCW 00 00 24 00 24.00 610 0.0191 11/19/2006 ERCWA & B & RCW 00 " 00 24 :00 24.00 610 0.0183 11/20/2006 ERCWA & B &RCW 00 00 24 00 24.00 '610 0.0086 11/21/2006 ERCWA & B & RCW 00 :. 00 24 : 00 24.00 530 0.0202 11/22/2006 ERCWA & B & RCW 00 00 24 : 00 . 24.00 430 0.0101 11/23/2006 ERCWA & B & RCW 00 00 24
- 00 24.00 430 0.0146 11/24/2006 " ERCWA & B & RCW 00
- 00 24 " 00 24.00 530 0.0056 11/25/2006 ERCWA & B & ROW 00 00 24 00 24.00 440 0.0129 11/26/2006 ERCWA & B & ROW 00 00124 00 24.00 460 0.0188 11/27/2006 EROWA&B 00 :00124 6-00 24.00
__________ROW .00:00 16:00 . 16.00 470 0.0138 ______ _ 11/28/2006 ERCWA & B 00 00 24 :i00 24.00 470 0.0120 11/29/2006 ERCW A & B 00 : 00 24 00 24.00 470 0.0288 11/30/2006 ERCWA & B 00 00 24 00 24.00 , 470 0.0376 _______________________________I F,_________________
I V I U1 VIII 1 uw Towerbrom 960. system injection injection duration in hours total quantity mass balance calc. Additional Results 1,425 lbs/day 0.10 mg/L 0.10 mg/L_ 12/01/2006 ERCWA & B 00 00. 24 00 . 24.00 RCW 11 42 16 00 4.30 480 0.0187 12/02/2006 ERCWA & B 00 00 24 00 24.00 420 0.0131 12/03/2006 ERCWA & B 00 00 20 00 20.00 230 0.0086 12/04/2006 n/a 12/05/2006 RCW 09 :130 13 55 4.42 20 0.0092 12/06/2006 ERCWA & B 12 25 16 25 4.00 220 0.0316 12/07/2006 RCW 10 20 14 20 .... 4.00 20 0.0104 12/0812006 n/a 12/09/2006 n/a 12M10/2006 ERcWA & B 15 45 19 45 4.00 170 .0.0257 12/11/2006 RCW 09 25 13 35 4.17 20 0.0117 12/12/2006 ERCWA & B 10.: 35 14 45 4.17 160 0.0317 12/13/2006 . RCW 09 45 13J: 55 4.17 20 0.0110 12/14/2006 ERCWA& B , 09 40 13 40 4.00 140 0.0232 12/15/2006 n/a 12/16/2006 n/a 12117/2006 n/a 12/18/2006. ERCWA&B 09 : 40 13 :40 4.00 140 0.0161 12/19/2006 RCW 09 : 30 13 :35 4.08 20 0.0118 12/20/2006 ERCWA&B 09 :.50 14 : 15 4.42 140 0.0198 12/21/2006 RCW 11 :115 15 15 4.00 20 0.0123 12/22/2006 ERCWA&B 09 :140 13
- 40 4.00 150 0.0167 12/23/2006 .n/a 12/24/2006 n/a 12/25/2006 n/a 12/26/2006. n/a 12/2712006 ERCWA&B 13 20 17 :20 4.00 RCW 09 25 13 :150 4.42 .140 0.0124 12/28/2006 ERCWA & B 121: 25 16 " 25 4.00 RCW 091: 30 13 '40 4.1 -7 160 0.0164 12/29/2006 n/a6' ..
12/30/2006 n/a 12/31/2006 n/a
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report PCL 401 Duration in Quantityin Pounds Hours Analyti Cal Results (lbs/day) (hrs/day) mass balance calculations (mg/L)
# of Days in *Month Maximum Average Limit Maximum Maximum Average Limit samples Service
-January 404 172 1480 24.00 0.033 0.014 0.2 23 23 February 108 81 1480 24.00 0.008 0.007 0.2 6 6 March 108 72 1480 24.00 0.009 0.009 0.2 2 2 April 108 105 1480 24.00 0.015 0.012 0.2 26 26 May 0 0 1480 0.00. 0.000 0.000 0.2 0 0 June 108 91 1480 24.00 0.008 0.008 0.2 5 5 July 0 0 1480 0.00 0.008 0.000 0.2 0 0 'August 0. 0 1480 0.00 0.000 0.000 0.2 0 0 September .0 0' 1480 0.00 0.000 0.000 0.2 0 0 October 0 0 1480 0.00 0.000 0.000 0.2 0 0 November 0 0 1480 0.00 0.000 0.000 0.2 0 0 December 0 0 1480 .0.00 0.000 0.000 0.2 0 0 Jan. - Dec. 62 62
PCL 401 PCL-401 system injection start time injection end time injection sta injection end time duration in hours total quantity ;mass balance calc. 01/01/2006_1,480 lbs/day 0:2 mg/L 01/01/2006 01/02/2006 01/03/2006 01 JOD412006 01/05/2006-01/06/2006 01/07/2006 01/08/2006 EB00 . 01/09/2006 ERCWA & B 00 00 24 00 24.00 404.06 0.033 01/10/2006 ERCWA & B 00 00 224t 00 24.00 404.06 0.033 01/11/2006 ERCWA &.B 00 00 24 00 24.00 404.06 0.033 01/12/2006 ERCWA & B 00 -. 00 24 00 24.00 404.06 0.033 01/13/2006 ERCWA & B 00 00 24 00 24.00 404.06 0.032 01/14/2006 ERCWA & B 00 00 24 00 24.00 107.74 0.009 01/1.5/2006 ERCWA & B 00 00 24 00 24.00 107.74 0.009 01/16/2006 ERCWA & B 00 00 24 00 24.00 107.74 0.009 01/17/2006 ERCWA & B 00 00 24 00 24.00 107:76 0.009 01/18/2006 ERCWA & B 00 00 24 00 24.00 .107.76 .
- 0.009 01/19/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 01/20/2006 ERCWA & B 00 00 24 00 "" ,24.00 107.76 0.009 01/21/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 01/22/2006 ERCWA & B .00 00 24 o00 t 24.00 107.76 0.009 01/23/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 01/24/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 01/25/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 01/26/2006 ERCWA & B 00 00 24 00 24.00 107.761 0.009 01/27/2006 ERCWA& B 00 00 24 00 24.00 107.76 0.009 01/28/2006 ERCWA'& B 00 00 24 00 24.00 107.76 0.009 01/29/2006 ERCWA & B 00 00 24 00 24.00 107.76 0009 01/30/2006 ERCWA & B. 00 00 24 00 2400 107.76 0.009 01/31/2006 IERCWA &B 0000 *o 1 24, 00 1__ 11 1___ 24.00, 107.76, 0.0091
PCL 401 PCL401 system injection start time injection end time injection star injection end time duration in hours total quantity mass balance calc. 11,480 lbs/day 0.2 mg/L 02/01/2006 ERCWA &B 00 00 24 00 24.00 61.96 0.005 02/02/2006 ERCWA &B 00 00, 24 00 24.00 61.96 0,005 02/03/2006 ERCWA &B 00 00, 24 00 24.00 107.75 0.008 02/04/2006 ERCW.A &B 00 00 24 00 24.00 107.75 0.008 02/05/2006 ERCWA &B 00 00 24 00 24.00 107.75 0.008 .02/06/2006 ERCWA &B 00 00 08 25 8.42 37.8 0.008 02/07/2006 N/A 02/08/2006 N/A 02/09/2006 N/A .... 02/10/2006 N/A ____._ 02/11/2006 N/A _ 02/12/2006 N/A 02/13/2006 N/A 02/14/2006 N/A 02/15/2006 N/A 02/16/2006 N/A __"_- 02/1*7/2006 N/A ___ 02/18/2006 N/A .. _ 02/19/2006 N/A, 02/20/2006 N/A: 02/21/2006 N/A 02/22/2006 N/A 02/23/2006 N/A 02/24/2006 N/A 02/25/2006 N/A , 02/26/2006 N/A _______ 02/27/2006 N/A 02/28/2006 N/A _
PCL 401 PCL-401 system injection start time injection end time injection star injection end time duration in hours total quantity mass balance calc.
"1,480 lbs/day 0.2 mg/L 03/01/2006 n/a. _
03/02/2006 n/a 03/03/2006 n/a 03/04/2006 n/a 03/05/2006 n/a 03/06/2006 n/a 03/07/2006 n/a : 03/08/2006 n/a 03/09/2006 n/a 0-3/1.0/2006 n/a 03/11/2006 n/a 03/12/2006 n/a 03/13/2006 n/a 03/14/2006 n/a. *___. 03/15/2006 n/a _ _ _ 03/16/2006 n/a _ _ 03/17/2006 n/a 03/1 8/2006 n/a ._ _ _ _ 03/1 9/2006 n/a 03/20/2006 n/a 03/21/2006 n/a 03/22/2006 n/a 03/23/2006 n/a 03/24/2006 n/a 03/25/2006 n/a 03/26/2006 n/a 03/27/2006 n/a 03/28/2006 n/a 03/29/2006 n/a_ _.__ _ _ 03/30/2006 ERCWA & B 16 - 00 24 800
.00 35.92 0.009 03/31/2006 ERCWA & B .00 -00 24 100 1 24.00 107.761- 0.009
PCL 401 PCL-401 system injection start time injection end time injection star injection end time duration inhours total quantity mass balance calc.- _. 1,480 lbs/day 0.2 mg/L 04/01/2006 ERCWA & B 00 00 24 00 . 24.00 107.75 0.009 04/02/2006 ERCWA& B 00 00 24 00 24.00 107.75 0.009 04/03/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 04/04/2006 ERCWA & B- 00 00 24 00 _ 24.00 107.76 0.009 04/05/2006 ERCWA & B 00 00 24 00 ' 24.00 107.76 0.009 - 04/06/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 04/07/2006 ERCWA & B 00 00 . 24 00 24.00 107.76 . 0.009 04/08/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 04/09/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.009 04/10/2006 ERCWA & B 00 00 24 00 24-.00 ..107.76 0.008 04/11/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.013 04/12/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.015 04/13/2006 ERCW A & B 00 00 24 00 24.00 107.76 0.015 04/14/2006 ERCWA & B 00 00 24. 100 24.00 107.76 0.015 04/15/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.015 04/16/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.015 04/17/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.015 04/18/2006 ERCWA & B 00 00 24 00 24.00 107.76 0.015 04/19/2006 ERCWA & B 00 00 24 00 " 24.00 107-76 0.015 04/20/2006 ERCWA & B 00 00. 24 00 24.00 107.76 0.015 04/21/2006 ERCWA & B 00 00 14 30 15 15 24.00 23.25 1.04.38 0.014 04/22/2006 ERCWA & B " 00 00 24 00 24.00 107.76 0.015 04/23/2006 ERCWA & B 00 -00 24 00 24.00 107.76 0.015 04/24/2006 ERCWA & B., 00 00 24 00 24.00 107.76 0.014 04/25/2006 ERCWA & B 00 00 ' 24 00 24.00 107.76 0.014 04/26/2006 ERCWA & B 00 -00 09 00 9.00 40.4 0.014 04/27/2006 n/a *_ 04/28/2006 n/a ,_,_" 04/29/2006 n/a 04/30/2006 n/a '
PCL 401 PCL-401 system injection start time injection end time injection star injection end time duration in hours total quantity mass balance calc.
" 1,480 lbs/day 0.2 mg/L 06/01/2006 n/a _
06/02/2006 n/a 06/03/2006 n/a 06104/2006 n/a 06/05/2006 n~a __ _ 06/06/2006 n/a 06/07/2006 n/a .06/08/2006 n/a ._*__ 06/09/2006 ERCWA & B; 19 10 24 lop 4.83 21.7 0.008 .06/10/2006 ERCWA &B 00 00 24 00 24.00 107.76 0.008 06/11/2006 ERCWA &B 00 00 .24 00 24.00 107.76 0'.008 06/12/2006 ERCWA &B 00 00 24 00 24.00 107.76 0.008 06/13/2006 ERCWA &B 00 00 24 00 24.00 107.76 0.008 06/14/2006 n/a & 06/15/2006 n/a "___ _ 06/16/2006 n/a 06/17/2006 n/a 06/18/2006 n/a 06/19/2006 n/a _ 06/20/2006 n/a 06/21/2006 n/a "_ _ 06/22/2006 n/a 06/23/2006 n/a _ 06/24/2006 n/a 06/25/2006 n/a 06/26/2006 n/a _ _ 06/27/2006 n/a 06/28/2006 n/a 06/29/2006 n/a 06/30/2006 n/a _ _. _
TN0026450 SQN 2006 Biocide/Corrosion Treatment Plan Annual Report MSW 101 Duration in Quantity in Pounds Hours Analytical Results (lbs/day) (hrs/day) mass balance calculations (mg/L)
# of Days in Month Maximum Average Limit Maximum Maximum Average Limit samples Service January .0 0 2280 0.00 0.000 0.000 0.2 0 0 February 0 0 2280 0.00 0.000 0.000 0.2 0 0 March 0 0 2280 0.00 0.000 .0.000 0.2 0 0 April 0 0 2280 0.00 0.000 0.000. 0.2 0 0 May 0 0 2280 0.00 0.000 0.000 0.2 0: 0 June 0 0 2280 0.00 0.000 0.000 0.2 0 0 July 0 0 2280 0.00 0.000 0.000 0.2 0 0 August 0 0 2280 0.00 0.00,0 0.000 0.2 0 0 September. 243 178 2280 24.00 0.018 0.016 0.2 30 30 October 243 197 . 2280 24.00 0.018 0.018 0.2 27 27 November 0 0, 2280 0.00 0.000 0.000 0.2 0 0 December 0 ,0 2280 0.00 0.000 0.000 0.2 0 0 Jan. - Dec. 57 57 Per the B/CTP approval: "MSW 101 is injected into the ERCW Train A & B system only during warm weather months."
MSW 101 system injection injection injection injection end duration in hours PHOSPHATE total quantity mass balance calc. 2,280 lbs/day 0.2 mg/L 09/01/2006 RCW 00 00 11 25 16 25 24 00 19.00 192.01 0.018 09/02/2006 RCW 00
- 00 24 00 24.00 19.4.68 0.015 09/03/2006 RCW 00
- 00 24 . 00 24.00 194.68 0.015 09/04/2006 RCW 00
- 00 24 " 00 . 24.00 194.68 0.015 09/05/2006 RCW .00 00 M 11 " 35 16 25 24 00 19.17 155.42 0.014 09/06/2006 RCW ý00 *00 11 10 16 30 24 00 18.67 151.44 0.014 09/07/2006 RCW 00 00 11 23 16 05 24 00 19.30 156.55 0.015 09/08/2006 RCW.' 00 00 11 00 16 40 24 00 . .18.33 148.64 0.014 09/09/2006 RCW 00 . 00 09 - 05 13 30 24 00 19.58 158.82 0.014 09/10/2006 RCW . .00 00 24 . 00 " 24.00 194.68 0.014 09/11/2006 RCW 00 00 10
- 15 15 00 24 00 19.25 156.15 0.014 09/12/2006 RCW' 00 00 09 - 50 15 16 24 00 18.57 150.55 0.014 09/13/2006 RCW 00 - 00 09 ' 10 14 00 24 00 19.17 155.5 0.014 09/14/2006 RCW 00 - 00 08 - 45 14 00 24 00 18.75 152.09 0.014 09/15/2006 RCW 00 00 08 . 47 13 45. 24 00 19.03 154.36 0.015 09/16/2006 RCW 00 " 00 24 00 _-24.00 194.68 0.015 09/17/2006 RCW 00
- 00 24 00 24.00 194.68 '0.015 09/18/2006 RCW 00 . 00 24 00 24.00 194.68 0.014 09/19/2006 RCW 00 00 08
- 30 13 15 , 24 00 19.25 156.15 0.014 09/20/2006 RCW 00 100 110 " 04 14 750 24 00 19.23 156.07 0.015 09/21/2006 RCW 00 " 00 10 - 40 16 7 10 247 00 18.50 187.58 -0.018 09/22/2006 RCW . 00 - 00 10 :.02 15 40 24 00 18.37 186.16 0.018 09/23/2006 RCW 00 . 00 08 30 12 735 24 00 19.92 161.58 0.015 09/24/2006 RCW 00 00 24 .00 24.00 194.68 0.015 09/25/2006 RCW 00 00 09 30 14 *45 247 00 18.75 190.11 0.018 09/26/2006 RCW 00 00 10 43 16 P06 24 00 18.62 188.79 0.018 09/27/2006. RCW 00 00 09 08 14 01 24: 00 19.12 .193.76 0.018 09/28/2006 RCW 00 - 00 09 7 06 14
- 30' 24 .: 00 18.60 188.59 0.018 09/29/2006 RCW 00 00 11 : 27 16 31- 24 " 00 18.93 191.94 0.018 09/30/2006 ROW, 00 00 24 " 00 24.ý00" 243.35 0,018 I
MSW 101 system injection injection injection injection end duration in hours . PHOSPHATE total quantity mass balance calc. I 2,280 lbs/day 0.2 mg/L 10/01/2006 RCW 00 " 00 24 00 _ 24:00 . 243.35 0.018 10/02/2006 RCW 00
- 00 .11 08 15 :55 24 00 19.22 194.78 0.018 10/03/2006 RCW 00 : 00 09 13 15 21 24 - 00 17.87 181.19 0018 10/04/2006 RCW 00 " 00 10 05 14 45 24 "00 19.33 195.99' 0.018 10/05/2006 RCW 00 00 11 04 16 7 36 24 : 00 18.47 187.28 .0.018 10/406/2006 RCW 00
- 00 10
- 15 15: 20 24* 00 1 18.92 191.84 0,018 10/07/2006 RCW 00 00 24 - 00 24.00 243.35 -0.018 10/08/2006 RCW 00 00 24 00 24.00 243.35 .0.01 10/09/2006 RCW 00 - 00 24 _ 00 24.00 243.35 0.018 10/10/2006 RCW 00 - 00 24 : 00 24.00 243.35 .. 0.018 10/11/2006 RCW 00, 00 11 : 28 16 03 24
- 00 19.42 196.91 0.018 10/12/2006 RCW 00
- 00 09
- 50 15
- 30 24
- 00. 18.33 185.85 0.018 10/13/2006' RCW 00: 00 11 " 23 16
- 24 24 " 00 18.98 192.44 0.018 10/14/2006 RCW 00
- 00 24 : 00 24.00 243.35 0.018 10/15/2006 RCW' 00 : 00 11 :20 15
- 45 24
- 00 .19.58 198.53 0.018 10/16/2006 RCW 00
- 00 11 " 01 16 : 22 24 : 00 18.65 189.1 0.018 10/17/2006 RCW 00 ' 00 10,: 12 14 , 48 24
- 00 19.40 196.70.018 10/18/2006 RCW 00 00 '09 32 14 : 45 24
- 00 18.78 190.42 0.018 10/19/2006 RCW 00 00 24 00 24.00 243.35 0.018 10/20/2006 RCW 00 00 10 .53 15 :25 24 00 19.47 197.31 .0.018 10/21/2006 RCW 00
- 00 24 00 24.00 243.35 0.018 10/22/2006 RCW 00 00 08 30 13 710 24
- 00 19.33 195.99 0.018 10/23/2006 RCW 00
- 00 09
- 30 14
- 45 24
- 00 18.75 190.11 0.018 10/24/2006 RCW 00
- 00 09 32* 14 703 24 00 19.48 197.51 0.018 10/25/2006 RCW 00 - 00 08 - 52 ' 8.87 89.94 0.018 10/26/2006 RCW 14
- 50 24
- 00 9.17 92.98 .0.018 10/27/2006 RCW 00
- 00 10
- 51 10.85 110.01 0.018 10/28/2006 n/a 10/29/2006 n/a 10/30/2006 n/a ___
10/31/2006 n/a
December 11, 2006 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2006. Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected November 12-17, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests. In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. Call me at (256) 38,6-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CTR.2L-M Attachment cc (Attachment): Files, RSO&E-EDMS-Muscle Shoals SQN' November 2006M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: December 11 2006
- 1. Facility / Discharger: Sequoyah Nuclear Plant TVA
- 2. County / State: Hamilton /Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 6. Design Flow (MGD): 1579
- 7. Recei'ing Stream: Tennessee River (TRM483.6)
- 8. 1Q10. 3491 9 Outfall Tested: 101
- 10. Dates Sampled: November 12-17, 2006
- 11. Average Flow on Days Sampled (MGD): 1569. 1565. 1564, 1567, 1557, 1563
- 12. Pertinent Site Conditions:
H-150M was injected from November 13-16, 2006. The dates and times for the H-150M injection are in the following table. H-1 50M was shut down on 11/14/2006 from 0812-0914 due to backwashing of the ERCW t-aveling screens and/or 1 50M tote change-out. Injection -Location " Date/Start Time Date/Ending Time (ET) (ET) Essential Raw Cooling Water 11/13/2006 1155 11/14/2006 0812 (ERCW) Train B 11/14/2006 0914 11/16/2006 1300
- 13. Test Dates: November 14-21. 2006
- 14. Test Type: Short-term Chronic Definitive
- 15. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Cerzodaphnia dubia)
- 16. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales-promelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6. 100 UV treated Intake: 100.0 -.
- 17. Permit Limit Endpoint (%): Outfall 101: 1CC 5 = 45.2%
- 18. Test Results: Out fall 101 : Piunephalesproinelas. IC-5 > 100%
Ceriodaphniadubia.. IC5 > 100% UV treated Outfall 101: Pimephalespromelas.'ICQs> 100%
- 19. Facility
Contact:
Stephanie Howard Phone#: (423) 843-6700
- 20. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 21. Lab
Contact:
Jim Sumner Phone #:J828) 350-9364
- 22. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 23. Notes:. Outfall 101 samples collected November 12 - 17, 2006, showed no toxic effects to fathead minnows or daph-nids. The resulting IC25 values, for bot-h species, were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different f-om control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in
.previous toxicity testing at Sequoyah.
2
NMETHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Infornmation:
Date Date Date (MM/DDiYY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Te'mp. TRC* Time (ET) ID Collected Received (°C) (m./L) Last Used By 11/12/060805 to 11/14/06 1425 101 11/13/060705 11/13/06 1450 23/20 010 11/15/06 1430 11/12/06 0840 to 11/14/06 1425 Intake 11/13/06 0740 11/13/06 1450 1.5 <0.10 11/14/06 08240t 11/15/06 11/16/06 1430 14309 11/14/06 0824 to 11/15/06 1425 4 .5 / 5 .0' <0.10 11/16/06 1409 101 11/15/06 0724 11/17/06 1355 Intake 11/15/06 0743 11/15/06 1425 2.4 <0.10 11/17/06 1355 11/18/06 1345 101 11/16/06 0753 to 11/17/06 1405 3 .3 / 3 .7' <0.10 .11/19/06 1330 11/17/06 0653 * -0'0 11 /20/06 13 1336 11/16/06 0814 to 11/18/06 1345 Intake 11/17/06 1405 0.7 <0.10 11/19/06 1330 1-1/17/06 0714 11/20/06 1336
*TRC = Total Residual Chlorine 'Samples were collected in two 2.5 gallon cubitainers Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.02C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were LTV-treated through a 40-watt Smat4-LV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. 3
Pinephalesprome/as CeriodaphniadL/bia Test Organisms: I. Source: Aquatic BioSvstems. Inc. In-house Cultures
- 2. Age: 22.5 - 24.5 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static. Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderatelv Hard Svnthetic Moderately Hard Snthetic
- 4. Number of Replicates: 4
- 5. Organisms per Replicate: 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101 11/14/06- 1425 ET 11/14/06 - 1218 ET UV Treated Outfall 101 11/14/06 1407 ET
- 7. Test Termination: (Date/Time)
Outfall 101 11/21/06 - 1328 ET 11/21/06 - 1132 ET UV Treated Outfall 101 11/21/06 - 1309ET
- 8. Test Temperature: Outfall 101: Mean.= 24.62C Mean = 24.92C (24.1 - 2 5 .12C) (24.6 - 25.3-C)
Test Temperature: UV-Treated Outfall 101: Mean= 24.72C (24.1 - 25 .12C)
- 9. Physical / Chemical were Measurements: ALkalinity, hardness, total residual chlorine, and conductivity measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
4
TOXICITY TEST RESULTS (see, Appendix C for Bench Sheets)
- 1. Results of a Pimep]hales promelas Chronic/7-day Toxicity Test (Genus species) (Type / Duration)
Conducted November 14 - 21 2006 using effluent from Outfall 101. Test Percent Surviving Solutions (time inter-val used - day's) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 ]00 100 11,3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2o 100 100 100 100 100 100 100 72,6% 100 100 100 100 100 98 98 100.0% 100 100 100 100 100 .100 100 Intake 100 100 98 98 98 98 98 Test Solutions Mean Dry Weight (mg) (% Effluent) (re licate number) 1 2 3 4 Mean Control 0.815 0.732 0.735 *0.778 0.765 11.3% 0.662 0.656 0.684 0,744 .0.687 22.6% 0.725 0.703 0.725 0.687 0.710 45.2% 0.679 0.657 0.650 0.563 -0.637 72.6% 0.662 0.622 0.684 0.660 0.657 100.0% 0.671 0.745 0.683 0.669 0.692 Intake 0.674 0.604 0.742 0.628 0.662 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit. 45.2% Pennit Limit: 2.2 TUc.: 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC 5 o: TUc = 100/ IC 25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniaduhia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted November 14 - 21, 2006 using effluent from Outfall 101. Percent Suirviving Test (time interval used - days) Solutions 2 3 4 5 (% Effluent) Control ' 100 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 .100 100 45.2%/' 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100. 100.0% 100 100 100 100 .100 100 100 Test Solutions " Reproduction (#young/female/7 days) TEsoluetis Data (replicate number)
.(%Effluent) 1 2 3 4 5 6 7 18 9 10 Mean Control 29 27 27 30 27 30 30 27 32 30 28.9 11.3/o 28 29 31 31 31 33 31 29 30 34 30.7 22.6% 33 34 32 28 31 31 35 36 35 30 32.5 45.2% 38 33 33 31 34 32 34 31 30 34 33.0 72.6% 31 37 31 36 34 32 35 33 35 34 33.8 100.0% 32 33 33 36 33 36 34 33 38 34 34:2 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA LowerLimit:- NA
*TUa =.100fLCso: TUc = 100/ IC2 5 6
TOXICITY TEST RESULTS.(see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus-species) (Type / Duration) Conducted November 14 - 21, 2006 using water from Intake Percent Surviving Test (time interval used - days) Solutions 1 2 3 4 5 6 7 (% Effluent) _ Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions . Reproduction (#young/fcmale/7 days) Teftolutonts Data (replicate number) " (%-Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 .26 30 29 30 29 28 31 27 32 29.1 Intake 38 30 34 34 31 33 33 36 33 34 33.6 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC 5 0 : TUc = 100/ IC 25 7
TOXICITY TEST RESULTS, lJV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pirnephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted November 14 - 21. 2006 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 11.3% 100. 100 100 100 100 10,0 98 22.6% 100 100 100 100 100 -_100 98 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 . 100 100 100 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (Og) (% Effluent) 1 2 (replicate 3 number) 4 Mean Control 0.809 0.735 0.7.89 0.766 0.775 11.3% 0.816 0.780 0.721 0.829 . 0.787 22.6% 0.726 0..939 0,804 0.739 . .0.802 45.2% 0.610: 0.814 0.712 0.836 0.743 72.6% 0.787 0.776 0.762 0.754 0.770 100.0% 0.705 0.737 0.710 0.772 . 0.731 Intake 0.646 0.768 0.717 0.772 0.726 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TTUa = 100iLC5 ) TUc = 100/ IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC 25 ) Pinephalespromnelas November 14 - 21., 2006 1440 7-days KCI 0.54 g/L Ceriodaphniadubia November 7 - 14, 2006 1127 7-days NaCI 1.06 e/L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values mnd Ranges for l'imepho/cs promelas and Ceriodaphniadubia Fests, Scquov;da Nuclear Plant Effluent (SQN), Outfall 101, Novemnber-14-2 ,2006. Test Sample II) Temperature CC) Dissolved Oxygen (mg/L) pH (S. 1) Conductance Alkalinity lHardness Total Residumil Initial Final Initial -Final Initial Final (Pmhos/Cm) (mg/L CaCO 3 ) (mg/I, CaCOj) Chlorine (mg/I,) Control 24 7 24.4 79 7.5 7.71 7.29 313 59 93 24.6 - 24.8 24.2 -- 246 T7. 8.2 7.0 79 752 -7.81 708 .758 308 -320 57 - 60 91 93 11.% 24.7 24.4 8.0 7 3 "7.55 7.27 295
._, 24.6 - 24.8 24.2 . 24.8 79 - 81 6.7 - 7.9 7.26 - 7 77 7.06 - 7.53. 289 - 306 ...
K Z* 22.6% 24.8 24.3 8.0 7.2 - 7 55 .7.25 282 2 6 24 7 - 2,t.9 241 - 246 7.9 - 81 65 - 79 727 - 777 7.01 - 753 273 - 296 *
- 24 8 24.4 8.0 7 2 754 7.27 254 --
247 - 24 9 24.2 - 24.5 7.9 ., 8.1 6.5 - 79 7 26 775 7.04 - 7.56 246 - 265 "Z 24.8 244 8.1 7 3 7.52 726 219 72.6%I 24.8 - 24.9 241- 248 80 - 8.2 67 - 8.0 7.24 - 7 73 7.08 - 756 211 - 231 100.0% 25.0- 244 8.1 7.2 7.51 7. 27. 185 66 79. <0 10 248 - 25 1 242 - 24.7 7.9 8.3 66 - 0 7.22 -771 706 - 754 175 198 65 - 67 77 - I <010 - -,010 24 8 24.4 82 7 4 7,50 7.27 185 65 78 < 0.10 24.7 - 24.9 24.1 - 24.6 7.8 - 84 6.6 - 80 720 - 7.71 709 - 755 179 - 198 64 - 66 77 - 79 <010 - <0.10 Control 24 7 24.9 7.9 8.0 771 7.49 313 59 93 246 - 24.9 24.6 - 25.2 77 -82 7.6 8.3 7:52 -781 7.27 - 771 308- 320 57 60 91 93 24.9 7 9 7.9 7.55 7.49 295 - 11.3% 24 8
.2 24.7 . 24.9 24.7 - 25 1 7 9 - 8.0 76 -_ 8.3 7 26 - 7.77 7.27 - 7 69 289 - 306 -
*. .g 22.6o/o 24.8 24 7 49 24.9 24.6 - 25.1 79 8.0 8- . 76 7.9
- 8 3 7.55 727 - 7777 7.50 27 - 7.68 282 273 -296 z . 45.2% 248 25.0 8.0 7.9 7.54 7.50 254
. 24 7 - 25.0 24 9 - 25 2 79 - 81 7.6 - 83 7.26 - 775 7.28 - 7.68 246 - 265 26 24.9 25.0 8.1 7.9 7 52 7.50 219 72.6 24 8 - 252 248 - 25 2 80 - 8.2 7.6 - 8.3 7 24 - 773 7.31 - 769 211 - 231 " -
250 24.9 . 81 7.9 751 7 51 185 66 79 <0.10 24.7 - 25.3 24.7 - 25 2 7 9 - 83 7 7 83 7 22 - 7 71 7 30 - 7 70 17 - 19 65 - 67 77 - 81 1 < 10 - < 0.10 Intake 24,9 24.9 82 8.0 7.50 7.50 185 65 78 <0.10 24 8 - 251 24.8 - 251 7.8 - 84 7 7 - 83 7.20 - 7 71 7 29 - 7.70 179 - 198 64 - 66 77 - 79 < 0 10 - <010 Overall temperature (CC) Average Minimum Maximum Pimephulcs promelas 246 '. 24.1 25 1 Ceriodophmni dubin 24.9 24.6 25.3 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimretphlespromelas Tcst, Sequoyah Nuclear Plant EfflucJnt (SQN), UV-Trcatcd Outfail 101, November 14-21, 2006. Test Sample ID Temperature ("C) Dissolved Oxygen (rag/L) pH (S.U.) Conductance Initial Final Initial Final Initial Final (@tmhos/cm) Control 24.8 24.5 7.9 7.5 7.62 7.34 302 24.7 - 24.9 24.3 - 24.6 7.8 - 8.2 71 - 7.8 7.49 - 7,83 7..11 - 7.59. 296 - 308 24.8 24.5 7.9 7.4 7.62 7.33 298
.3 24.7 - 24.9 24.1 - 24.8 7.8 82 7.0
- - 7.8 7.48 7.83 7.11 - 7.53 288 - 304 t*22.6% 24.8 24.5 8.0 7.4 7.62 7.32 283 24.7 - 24.9 24.2 - 24.7 -7882 6.8 '- 7.7 7.47 - 7.82 7.10 - 7.55 276 - 293 24.9 *24.4 8.0 7.4 7.62 7.31 257 45.2%
24.8 - 24.9 24ý2 - 24.7 79. - 8.2 6.8 - 78 7.44 - 7.79 7.11 - 7.55 248 - 267
-7 7 2.6/24.9 6%'. 24.4 8.1 7.4 7.60 7.33 221 24.9 25.0 25.0
- 24.2 24.5 24.5
- 7.9 8.0 82
- 6.8 7.3 7.8
- 7.43 7.58 7.77
- 7.11 7.33 7.55
- 216 -
191 230 24.6 - 25.1 24.2 - 24.7 7.9 - 81 6.8 - 7.9 7.39 - 7.74 7.13- 7.57 188 - 202 24.9 24.4 8.1 7.3 .7.55 7.33 189
._ _n_
_ e 24.8 - 25.0 24.2 - 24.8 8.0 - 8.2 6.9 - 7.9 7.29 - 772 7.07 - 7.58 184 - 195 Overall temperature (°C) Average Minimum Maximum Pimephales promelas 24.7 24.1 25.1 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected November 12-17, 2006, showed no toxic effects to.
fathead minnows or daphnids. The resulting IC2 5 values, for both species, were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the sispected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. 1]
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-82 I-R-02-013 using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mLplastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropy.lene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCO-L
- 1. Piniephalespromelas None
- 2. Ceriodophniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pin'ephalespromelas None.
- 2. Ceriodaphniadubia None 12
PHYSICAL AND CHEMICAL IETHODS I Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration informtation pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by EPA Method 170.1.
- 4. Dissolved oxy gen was measured by EPA Method 360 1.
- 5. The pH was measuired by EPA Method 150.1.
- 6. Conductance was measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
- 9. Total residual chlorine was measured by ORION 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism
*culturing/acquisition and acclimation, test organism handling duringtest, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study, and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart inforniation) I. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaC.
- 2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.
Sodium Chloride (NaCI crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES I. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimatin2 the Chronic Toxicity of Effluents and ReceivinR Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA-600-4-79-020 (March 1983)
- 4. Qualitv Assurance Pro-ram: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nucl'ear Plant Biomonitonng November 14-21, 2006 Appendix B Diffuser Discharge Concentrations of Chemicals Used to Control N'Iicrobiologically Induced Corrosion and Mollusks, During Toxicity. Test Sampling,
Table B-1I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge ConcentratIons of Chcmicals Used to Control Growthof Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Daie :*.:: ::Sadityr Towerbrorm PC1-222 PCL-a01 CL-36* 3 Cuprostat- ,I413qM Hjcoi mg/L rngfLL rpiL 11-9/I PF mg/L Qa
.mg/L .. ... .phosphaze.
. .TRC Copolymer
... . ... ... DM1,A D A zP-,
mr'olt 03/12/1998 0,016 03/13/199S 0'015 - 03/11/199S - 03/16/1998 0'0.030 03/17/1998 f 0020 - 03/18/1998 10018 - 09/08/1998. 0 ý0 1 OA 014. 0,005......... 0:021 09/09/1998 0.0f03 00031 0011 09/i 10/199s i.0 14 00,6ý0 0021....... 09/11/19981 (),( I - D'05 0019 09/12/1998: <( 00U1 -0,044 00O15 09/13/1998 < G001 -ý 0.044 0015 09/14,/1998 0.008s 0,044 (1 0 015 ___ 02/22/1999 < n::::.<000 02/23/1999 0).405 02/24/1999 :..7 .a9* 02/25/1999 .:: 0. 02/26/1999 0,008 02/2 7/11999 <0.001 1021/28/1999 0-6O0G1 1 __________ 08/18"1999 -0015 i01069 0.024 0.006 08/19/1999 0.12 0.,0 68 0,024 08/20/1999 0.23 0.070 0,024 - 012 08/21/1999 0.22 0'.468 0,024 -' 08/22/1999 0.22 0,(16S 0.024 - 08/23/1999 0.25 0.068 0.024 0,00M6 108/'24/19991 ______ 0.16 1 0 f 67 0.023 0,f)020 _____ . 11 - 7
Table B-I (continued). .Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - Decemberf 1 2006 Date SO*::i:umf Towerbrom :iKP.L-2:2 PCL-401 CL:-3:3 Cuprosm- 1-11 3M Hy*poehimoritc mg/L :,Ii m:::S:i mg/L mngL PF g/I,'I mg/L. TRC phcsphalte Copolymer DMAD mig/L Qu~t __I-PITC Azole 01/3!/200 0002 0 02 00090 02/01/2000 0.011 0.0326 0028 02/02/2000 0.02S 0,026 0.009 0.0 ... 02/03,,2000 0.008 0.02' 0090 02/04/2000 0.006 0.0r2 7 0.009 0.0S. 0.109 : 02/05/2000 < 0.002 0,027 0.009 - 02/06/2 000 < 0.002 D.027 0.009 07/26/2000 < 0.0057 0J.*S5 0.019 07/27/2000 0.019 0.i:55 0019 - : 07/28/2000 .. 0.0088 0.053 0.018 .0,004 0108 07/29/2000 < 0.0088 0055 0.019. 07/30/2000 < 0.0076 0.O5s 0.019 07/3 1/2o000 <00152 0o055 0,019 O-O0.6 08/10 1/2000 <00 14! 0,055 0.019 0,;005 ___ T2/1 L/ :0143 0.ii f),02:5 0.020 : 7:0:05 12/12,/2000 0.0 0092 0.0005 0.0::2::::5 0.020 12/13/2000 < 0.0120 41,025 0.020 12/14/2000 < 0.0087 0.025 0020 12/15/2000 0.0120 0 02ý*01 : 0.020 0G.i 5 12/16/2000 < 0.0036 0.0*ý : 0,020 12/17/2000 < 00036 0.0o25 0020 =_
=_=__====___
08/26/2001 0.017 1:06 0021 0.0.406 08/27/2001 <0,0096 :0 :06 0.021 0,0*05 - .0:21 08/28i2001 <00085 0,0t6 . 0.021 . - - 08/29/2001 <0 0094 005:0. 0.020 0,005 - : 2: 08/30/2001 <0.0123 0,06 0,021 0. 00 IS 08/31/2001 o _______.___ < 005 0 D,059 0,00 20.: 111/225/2001 <0 0044 :. -. 11/26/2001 . <0.0119 0.024 002 0.0,5 11/27/2001 ..0137 n023 0,019 0.0071 11/28f2001, <O.0009 0:022 0.019 0.:t6 6 11/29/2001 0.0132 0, *-.4 .* 0.02 0.007
*11,/30!2001 0003
<______ 0.24 0.02 ____
12/09/2001 <0.0004 2 12/410/2001 . ~ <00042 -- 12/131/200 1 <0.0104 " 12/12/200.1
/0.0128 0.024 0,02 0o008 2/13,2001 <0.0088 Q,00*214 00.2 t 12/14/2001 , 00134 0Q24 0.02 ,.,007 _
3
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to-Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date Sodh*nyl Towerbrom PnCL 222 PCL-401 CL-363 Cuprosiat- ýHII30N Hypoc:chiorite, m giL ng L m g/L ing/L PFF n-g/L
- mg/l TRPC FPh(sphzteý Copolymej DMAkD ngL QUAt
______ TRC ____________Azole 01/02/2002 -< 0 0079 0,.02 3 0.02 0-006 01/03/2002 < 000042 0,.R23ý 0,014 0 01/04/2002 0.0124 f).0(2 4 0,014 0009 01/05/2002 < 0.0042 :: . 01/06/2 007- < 0.0042 . 01/07/2002 <0 0089 CI,(C4 0,014 0.006 02/24/2002 < 0.004 - : 02/25/2002 < 0 004 00.023 0% 02/26/2002 0.0143 0023 0023 0,007 02/27/2002 < 0 0041 0,023 0 023 02/28/2002 < 0 004 1 0,024 0,008 03/01/2002 < 0.0041 0,024 0.008 __ :._. 05/05/2002 *. - . - 05,/06/2002 -05 0.02 0 0-f4 05/07/2 002 -0,05k 0.02 O-Of5 05/08/2002 0,05 6 0.019 05/09/2 002 0.7 0, 02 0.02 4 05/10/2002 ______ 0,056 0.019 ______ 08/04/2002 <0.0058 - - OS/05/2002 <*0058 .,05 00018 (02 iu8/06/2002 0.0092 0,053 0.018 08/07/2002 <0.010 7 ti5 5 0.019 010)07 03/08/2002 <0.0061 0,05 5 0,019 08/09/2002 0.0152 0,054 0.018 30ý0ý8 __ 10/06/2002 <0,004197 - 10/07/2002 0.0153 0,054 0.018 U,009 I10'08/2002 <0.0092 0,054 0.018 0007_ 10,'09/2002 0.0124 0.05.3 0.018 G0009 10/10/2002 0.0134 . 0.054 0.018 D009 10/11/2002 _______ <0,0042 0.054 0.018 J_ ___ 01/12/2003 *<0,003 5 . - - 01/13/2003 <0.006 0.0?51 0.019 G04 099 01/14/2003 <0.01 18 0026 0.020 01/15/2003 <0,0063 0,076 . 0,020 0.0 01/16/2003 <0,0034 00,216 0.020 01/17/2003______ <0-0034 0.026 f) 0.009 04/0 6/20 0 3 <0.0073 - 04/07/2003 <0.01 89 - 0.021 04/08/2003 <00117 -0.021 04/09/20 0 i <0.00139 - 0.021 0,01. 04/110/2003 <0.0113 0021 0-018 104/I 1/20031________ <0.0073 1 0,022 ____ _________ 4
Table B-I (continued). Sequoyah Nuclear Plant Diff-user (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of MicriobiologicallI Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12. 1998.- December 1, 2006 Date Sodituct Toverbrom PCL-222 PCL-40] CL-363 Cuprostat- H4-130M* H-ypcchiorite mgL mgýL mg'L PF mg/L rglL TRC Pbospi~atte Copolymner EDMAfl mg/L Q~ TR C Az70!e 06/15i2003 <0045 06/16/2003 - <00037 0.057 0020n2 06.7!'2 003 <000048 0 .(41 0.014 - 00",02a 06/18/2003 < 00048 0,041 0~0014 .. ., 06/19/2003 < 0 0085 0,:
.$() 0 020 -
06/20/2003 < 0 0048 0.,1 : 00 0.02S 002 08/03/2003 <0 0050 0S,'04/2003 <0,0050 0.05 0.020 0S/05/200 3 <0,0051 0, 057 O0202 0.025 08/062 003 <0 0084 0.057 0.020 0 -025 08/07/2003 0.0129 t0tO5 0,020 0 024 08/o6'2 003 00153 0007 0020 . . 10/05/21003 <0.0043 0,07 0.020 10/06/2003 <0,0043 0 .0P257 0202" 0.025 10/07/2003 <0:0090 0 05 0020 0 -025 IO/0t8/200': <0.0 10 6 0,7 . 0.020 0.025 10/0912 003 0ý0181 0, 02ý 0.022 - 0.025 10/10/2 003 0.0I83 0. 026 0.024 -,0ý 02/01/2004 0H093 0..0090 0 02/02/2004 <0.0034 06 009 0.020 02/03/2004 <0.0034 026,026 0.009 021/04/2 004 0,0124 0,0 26 0.009 000-(9 02/05/2004............ . 0034 0,024 02009 02/06/20040015 0.0103 ,6 00009 00T 051/04/2004 <0.01 23 4),0N6 0.019 001 05/05/2004 <000144 0.0(1426 0014 009 -0 (025*5 05/06/2004 <0.00146 0,037 0.0139 - 0,02 5 05/07/2004 . .4,8 0 0227 0.020 0.009ý - 5* 5 05/08/2004. 0016 (40 0021 - ... 05/09/2004 <0. 0104 S.5 .0.020 - - 07/04/2004 -0,0217 0,.C57 0.019 07/05/2O004 <0.0085 0.:0Ci57-, 0 020 . 009 . 07/06/2004 <o00077 0A)59 0.020 - 0-031 07/07/2004 00252.0
<0.025019 0 93 ..
07/08/2004 <00223 0 O*.57 0019 0 09 . 07/09/2004 0.0182 0.0,5 -0.020 0009 5
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Conceinnations Of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling. March 12, 1998 - December 1, 2006 Date Sndium Towerbrom PO.*.Z2 PCL-401 Cb..363 Cuprostat-PF *)*.OM Nalco .HWIt0M.t Hypocbho~it mg/L mgJL mg/L mgL mgiL n1-/ 7355 1 TRC Phosphat-t Copolyrner D :MADAzole Qual mg,"L Ua:t
.. . .~.
. .Eo,'PO TRC 1/071'.2004 <0,0187 0..CI.0 0,014...
1lt08:-04 <0.0192 0 0(47 0.030 : 1 1/09/2 004 <0.0233 i-4 0 0.00016 0.041 11/109,2004 <0149 00047 0.016 0.041 1/011/2004 <0.0149 0.9*9 0.017 :0*043 11/122004 <0.03 0253 0017 :.2.. 02/06/2 005 <.0,0042 0.028 0.010 02/07/2 005 i <0.01 16 0.02 0,0100.7 02/08/2005 <0,0080 0,02 0,010 02/09/2005 0.0199 0.0G28 0.010 02/10/2005 <00042 i02, 0 010 02/11/2005 0.0155 0.028 0.0100.7 06/05/200 0.0063 06/06/2005 0.0043 06/07/2005
- 0037 0.0103 : 0.037 06/08/2005 0.0295 .. . . 1 06/09/2005 : 0.0129 :
06/10 200 0.0184 07/17/2005 .. 0.0109 0,02 0.009 07/18/2005 0.0150 0, 026 0.009 " 0-.036 07/192o005 0.0o163 0.(26 0.009 : -. (036 07/20/2005 0.0209 0.02,5 0.009 -0.014 0.036 07/221/2005 0.0242 0, 02,5 0.009 " 07/22/2005 0.0238 0.0.54 0,018 0ý014 10/30/12005 0.0068 10/3 1/2005 -0.0112 11/01/2005 0.0104 ( H)035 11/02/21005 0.0104 11/03/2005
.. 0036 0.011-7 G0036 11/04/2005 D35 .0.0165 11/15/2005 0056 0
11/16/2005 -0.023 11/17/2005 0.023 11/18/2005 0,020 11/19/2005 .0.016 6
Table B-I (continued), Sequoyah Nuclear Plant Diffuser (Outfall i0]) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria. and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 J-bl5OM Sodliun schbtim Towerbrom *e PCL-222 PCL-401 CLopolyme PCL- 401 CL-363 Cuprostai-PF -H-13 MA Nalco H- 15W.1 ypocblorit mg/L mg[L mgrL mg/L mg/tL 73551 e TRC ii~ii~ Rigiii~iiiii'iii~t Copolymer DIA.D Azole Qa mg/L M&I EO/PO TRO .~ 11/12/2005
*1- 0.0055
- 9- -.---
11/13/2005 0.00.68 11/14/2005 0.0143 0.037 11/15/2005 0 0068 11/16/2005 0.0267 11/17/2005 0:0222 I 1/26/2006 - 0,0188 1 1/27/2006 0.00138 1 1/28/2006 0.0120 11/29/2006 0 00288 11/30/2006 0.0376 12/01/2006 O.0 8 7i:: ?i::::ii l#:i ] 0.0187
}ii:i~i~i~i?:iii ii:il~{:{iLl i~{}i~~i~
7
Sequoyah Nuclear Plant Biomonitoring November 14-21, 2006 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page _I- of ý Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier. Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identiflication / Grab/Comp. Collection Date/Time Container Flow R,-* E n ' i. -- *Ov Sample Description Number & MGD Rain Event?Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes. No Trace ETS Log Arrival By Tome Appear-
......... Inches Number Temp. ance
*__"_(oc) _
SQN-101-TOX Comp 11/12/06-11/13/06 0805- 2 (2.Sgal) NA X , .- z C 0705 " SQN-INT-TOX Comp 11/12/06-11/13/06 0840- 1 (2.5 gal) NA X SQ-N-O op F0740 0o11L32" .", \: 5 Sample Custody - Fill lIn From Top Down ,A , . 5V"-QL, Relinquished By (Signature): Date/Time Received By (Signature): Chevy-Williams I11/13/06 /(_
' . Express Courier 11/13/06 ,
r Express Courier 11/13/06 ETS 11/13/06
/
tA7o 04.,t Instructions: Clients should fill in all.areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storingthem at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, pleasc collect and ship in such a way that the laboratory will receive the s;unples with ample time to initiate tcsting within that time frame. Samples shipped overnight on F.riday via FedEx or UPS must be marked for Saturday'delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of - Client: TVA Environmental Testing Solution, inc.. Delivered By (Circle One) Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Chent P.O. Number: N/A Asheville, NC Other (specify): Express:Couriei 28801 General Comments: Facility Sampled: Sequoyah NP 04 NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 ______ ____a Chevy illiams 11/15/06 Express C rier 11/15/06 Express Courier 11/15/06 ETS I11/15/06 "1 -0 -A .. _ _ ILI_15 Instructioins: Clients shoutd Fitl in all areas except those in the "Laboratory Use" block. Biomonitorintg samples ai c preserved by stoning them at 6°C and shlipping them in ice The hold nine 1k samp)le is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory wi4i1 receive the samples with ample time to initiate testing within that time frame. S shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or thiey wvill not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequ.oyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Express Courier I1/17/06 ETS 11/17/06 1/ -
'./, / 'Al
(. 7 /7 ~ .1 ~ ~-L1-r-t(, \'WSz, Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Diomonitoring samples arc preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Th'ercfore, please collcect ad ship in such a way that the laboratory will rcccivc the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Environmental Testing Solutions, Inc. Page I or6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA County: Hamilton Facility: Sequovah Nuclear Plant Treatment: Non-treated NPDES 9: TN 00264-50 Outfall: 101 Project 9: 74-_8" Dilution preparationinformation. Comments.: Di uten prep (1) 1 3 22 6 452 72 6 100 Effluent volume (mrL) 282 5 66 1130 1815 2500 Diuen fvofme (mL) 22 17 5 - 0 1370 6S5 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information. Organism'age.: 2 -."o
-Tz o', 'o.
O.I Randomizing tempJate e"UtoL.) Date and t-mes organisms k\. tc) 40 Tr "-c' 1,-o Incubator number: were born between: Organism source: AtS &T('t-( PAi* to " Artermia lot number: 66 12oq U Transfer bowl information. pHl Temperatr-e = C Total drying timeý 2'4.'S AoiOfz
" 7.. Date/Time in: tl-zi-o t ,, ,,5 0 Average transfer volume: g * -Date / Time out: t .-d, I-AoO Oven temperature. -
o00 Daily feeding and renewal information. Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time k . 0 q-Oo * -00 2"5 O \A O (lol) .01':-L2.. 2 -l- oj tboo It<,o% tA '-- O7.- 60 lllc .OsC), 0)'-.L.
, tL- OG oIi ~ _______[ O V"?O'-* . I\t-\'%-CQ l P\ lt\ *'I . isA*'l3 '/
4. 5 8- 0.0 I O I- Il k, O 0 C\'-$
% ( cc tP-I 1.o i c.
6 20 O(C%
'I- C, I t: 0 -" ,- s- -.. Oz 0 it I 7... .. ". h1 ... .: .
Control information: Acceptance criieria Summary of test endpoints:
% Mortality: !5.
*20% 7-day LC 50 > OO-7, Average weight per initial larvae:. O.- "l :y: jI NQEC '<1.3%
Average weight per surviving larvae: O.5 , >-025 mlarvac LOEC I1.30'7. ChV A < 11.37. IC25 I ?to7 Cc 5 I'JretkXUPr Eb bose keS-O e~~Ae-e L)J1&_L1~t-e
, -ý. - UtIU Species: Pimephalespromelas D3ate: Vl\-LLL-cjlto Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6%
A B _ _ _C D E F H I J K L 0 10 10 10D 0D 0.. /0 /0 C0 /0 /0 /0 /(D 1/D /0 /0 /0 /0 /0 /0C) /I '0 // /0 2 D0 (0 /t /0 /0 /0 f1Q /t /0 /) i /0 3 1D /0 10
'0 (C /( /0 'C /O oO /t /0 4 0 CQ 0*0 10 /0 *D 3 1 '
/0 /0 10 '0 1
IO /00 0 10 /0 1D 10 10C) 1I IC ( t/0 6/ /0 10 11 /0 1o l05 tO /U 10 /C /t [ IO a b / /D /0 /0 /Dt tO 10 (0 A = Pan weight (mg) Color identi ication:- "rt.A, iq.as j.V7 i q. a 1q. 13.rl7 13 c r 7 Iq.qb lqHAS 15.00 13.4bt Nq. A.nalysy :: .. B = Pan +-Larvae ,eight a o olo a l.17S Analyst: m A Bl.S o Larvae weight (mg)=A - B 3,T YL.2 .< .o .* *.~q 7q .- 0 70 ,% V*' Weight per initial number
- 4, C) C.,
of larvae (mg) A 'V ,%. 0% = C/Initial number of larvae Ct" o" 0* 0' 0' D cy A, C. Average Percent weight per reduction initial from control (o 1l., 0) oý)a1 ()-1 1.-7 number of (/) a2r'ae (mrg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, tn = missing, sk sick, sm - unusually small, 1g = unusually large, d&r = decanted and returned, w wounded. Calculations ard data reviewed . CoAns T) 0T6IXPJ iC ZtoL,1 4 WAS, t\)oT OcUt4P_ jr~
% Ht U)cu
)pKJ IAe- eFLýX3 O&,tJIT71 MJ 71ZPATHekjT$.-- A1~--
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pirmephalesprometas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: V-1Ki, Survival and Growth Data Day 45.27 72.6/ /00% NTT P Q R S IT F vw X 0 j 100C) I0 ' 0 0 /0 1c) C) IC 0 C0
/0 0 /0 /0 ) /0 /0 /0 tO 2 10,0 /0 0 /0 /0D /0 /0 "0 /0 /. ./0 to /I I0 /0 0 ,' /o /0 /0 ,'/ 'a 4
,o * /o
'0 ,o ID /0 tO /0 1 /C0 /0 5
0 C)O 0 10 I10 0) O tC101 I 1 10/0 6 o /0 / : ' 0 /O /1C /0 /0 /0C) 7 i~ D 10 1D 10 /00l /0 10 /0 /D 'O A = Pan weight (mg) Color identification: Ltlikr "TX &.'.t e.'0 15S.-Y7 -N-,3qq 13.%o l .S 13.4S 1/. 1 14. 19 13".O 80 R a 13. ýq I 4.0,ý 13.(4,3 Analyst: IJ:f B = Pan + Larvae weight Analyst: U4.[ *:*1o* .~: xt o 0 i~ .a S ,.,40 ao.*ý augt 30. S):o Larvae weight (mg) = A - B of larvae (rag) Weight per initial number _, ' ,y '0 ,\\ -.
=Ct/ nitial number of larvae , \.0,*
a "a
- D I' o' cY " " U Average Percent weight per reduction initial from control 0 .{o3 -1 *. .. ,.
number of (%) larvae (mg) Comment codes: c =clear, d dead, fg fungus, k killed, M missing, sk sick, sm unusually small, Ig= unusually large, d&r = decanted and retummed, w ,Y wounded. Calculations and data reviewed
1-'ace 4ot 6 Species: Pirnephalesprornelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 1-0-,-cl, Survival and GroKth Data Day 100% Intake Y Z AA BB 0 0 !0 (Q '0 /0 2 /0 /0 D0 /0 3 IL) IýA 0 /0 4 /o 1i o /0 5 6 7 S H I iD
*o ID / /0 A Pan weight (mg)
Color L ' -tr'. idenitification: -Tcrt.*i 13.S;S I f'. 0 13.3ý 1315-T Analyst: B = Pan + Larvae weight T Larvae weight (mg) A- B Weight per initial number ofl4arvae (mg)
=C / Initial number of larvae "o Average Percent weight per reduction initial from control " .
numberof (of ) larvae (mg) Comment codes: c clear, d =dead, fg fungus, k killed, m =missing, sk sick, sm unusually small, Ig unusually large, d&r decanted and returned w = ounded. Calculations and data reviewed6
Non-treated November .14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Mtthod 1000.0) Species: Pinephales promclas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2878 R t eircd b) , QC OCo,nrr*rlor (V.) H~ptku,, (ja C.ftlunrit,, in. u b. "1oWdFic.*",Lra, rt.'. A-Pnib A-M..el
- . fih¢q rnl B P.
Ile.f.ri.1
-P..I
*_Nor L, .ll'(ohI. ý"
f.o Corrrfaiacc Assre...Rt datii . Soani WIN -of.
- M.al,,Srtr M,.'tbOof3,,( hI IS-"ý1 Ir Loboiairy Cir~ro C.II f 1*. tl ~r~an
-0.1111 Sriljr.
ucoda.*b WlI[hlI INIm0 o1Mb bo bI ~ , srot-.. M.*' *-
- r*I I, ocloIkCo.)
1 If, .. .te rIW C., n 1,* of Arso P(Mrn'2iai F,* .rd '
,,r,Ithi,(rr ) -A Hi n-rb, tI .e , (I. r- , i o, ., re).).ai r - ,,a (.C A 10 10 25 22.10 8 15 0 85 os0.5 0765 5.2 t2nI app1i
-C Br 10 10 10 10 (4.47 14 22 21.79 21.57 7 32 7.35 0 732 0 735 0.760 5)2 073520.0 D 10 10 14 99 22 7) 7278 0.778 0 778 E (0 (0 (3 20.40 6.62 0.662 0 662 11,3". P (0 10 13 97 2053 6 56 _ _0656 067 S9 0656 100.0 0.687 5.9 103 10 10 14,980 2(82 6 8' 0684 068494 0I , 100 1(445 21.( 9 7 44 0 744 . 0.744 (0
1 (0 15 00 22 25 72_5 0725 0725 22.6", 2 (0 10 1298 2001 7.03 0703 07(0 26 0709 108.0 0.7( .2.6 72 K (0 10 . 1346 2071 7.25 0725 0.725 10 (0 (4 88 21 75 6 87 0 687 0687
.",(0 (0 15.37 22 16 6.79 0679 0 679 45.2%°7 N (0 . o 14.72 21 29 6 57 0657 0.607 80 0057 (00.0 0.6J7 8.0 16.7 0 10 (0 13 76 20.26 6 50 0 650 0 650 P (0 10 14 75 20 32 5 63 0 562 10S63 Q 10 9 (348 20,10 662 0736 0662 72.6% /. 10 10 14 21 2043 6.22 0,622 0675 70 06?2 97.5 0.657 3.9 14A 1(0 (0 14 12 2096 6 84 06894 0684.
T (0 10 13 80 2040 6.60 0660 0 660 (0 (0 14 12 20 X3 6.71 0671 0671 100% v l( 10 13 69 21 14 7245 0745 0692 52 0715 (000 0.692 52 95 W a0 (0 14 02 2085 683 0.683 0 683 X tO 10 13,42 2011 669 0669 0669 Y 1 10 ( 55 2029 6 74 0 674 0 674 100%!,(nake z_"__t_ ( 9 160,4 2208 6.04 0.671 0679 6 0.65 0604 97.5 0,662 9.2 AA 10 (0 (3.30 2080 s7 42 0742 0 742 B(B 10 1o 1) 75 2003 6 28 0628 0620 OuINJIl 101: MSD = . Minitsim Siprfican) Dafference Dunntrne' MSD value: 0.0625 PNMSD= Percen i MtnterrcSa*tafacanr Saference it PMSDi 0.2 PMSD is a'i nearuri o( test prr.iaora "l'it PM SD is Ie rituJtirn perc entl dffer en rcbiweei,aihe cont rol and al(real I meal that cani be declared I "tl ,I a)t y sily aficat in a who Ie efnuatva t) ;I On avera ge. a irpaific aol di frreac e occur, (or learm
,I.ionrrerai k Solar at III chronc ro .iniy (calf when a Io. coal raedi cc Pirerph nler rovclh by 15 0% from Ihr l onirol (d elrran, Ira ake:
through re(ec'ance toxicant teslulg). Durnell's IMSD vilu:e 0.09707 Loer PMSD bounad de-unied by USEPA (10th petcera(5) = 9,4%. PMSD: 9.) Upper PMSD bound dtccro,.d by USEPA (90rh percenlile(' 35% The lower and upper bounds were calculat(ed by (he USEPA using 205 (Call coriuntacir frorra 19 labo,-alorCS for PThme 1 Trrp otw*l*t chronic tefereocr tox~ican tests 7 USEPA 2000. Underslanditg auI AccouTWmg for Meihod Vaiabdtiyit \Vhol(e Effueoi Toxicity Apphtcatonn Under the Nationai Pollulant ( rcharLi lunimatio Pro*arm EPA-83 -R--00003 US Eaicrcirna Pro-icitor Agency. Cwtuncnrai, OH
ion-treate( November 14-21, 2006 Statistical Analyses Larval Fish Gro-1h And Survi-l Test-7 DAY Gro%-Lh Stan Dais H1/14!2006 . Tesl ID PpFRCR 'a;'.pi, iD TVA/ Sequoya Nuc-a4 PiarJOulfis1101 End Da:,. 1112112,)06 Lab ID ETO Ennir. Tes/aigSol Sanple TypD DMRDischiatge MoniorIiuig Repo S.mple Dv!i ' Pri/ican. PWCHIR-EA-821 R02-013 Te, Species PPPmepliak, pcomclas C.*nniments Nm/n.tea/ed Cona.% 1 2 3 4 D.Con:,1i) 0 8150 0 7320 0 7350 0 7780 H 3 06620 0.6560 0 6340 0 1440 22 6 0 7250 0 7030 0 7250 0 66870 45 2 0 5790 06570 06500 0 5630 726 06620 00220 06340 06600 1CG2 0.710 07450 06130 06600 Transform: Untr-nslormed 1-Tailed aIatisa Cons-/ NJ-e N-.ean Mle-n Mdin M", CV'% Ni s-Sau Csifia.l ,ISD J Mean Nfi-en D Conn,.l 0 7650 0Cox 07650 0 7320 0 8150 5 151 4 0 7650 C000S 113 06860 08974 06E65 0,6550 077440 5853 4 3029 2410 0.0625 06983 0.9127 226 07100 09281 07)00 06370 07050 2637 4 2122 2410 00625 069F3 09127
'45 2 0 6373 0.830 0 6373 0 5530 0 6790 8006 4 4 929 2 410 0 0625 0,6621 C 8655
*726 06570 0 8588 D 6570 0 620 0630 H 3 a1 4 4 167 2 410 0.0t 25 0 662 0 8655 I O, 06920 0 9046 06920 06690 0 700 !8 64 4 2 316 2 410 0,0625 0 6520/ 0C065 aui ra, Tes a Seuansd Critic Sk,. Kurl Ship ,-W-.s Tý;ttaiOats norms) 6b6 in (p0>.O0G) 0966335654 0 FA -0.0204166 .012457)70 Banleks Teal id,caien eqaal *.aaianccs (p 0 70) 2 993674904 15 08627224 Hnpohseais Tet (l-al, 6.00) NOEC LOEC ChV TU NISD u ISUp OISB OISE F-Piob d(
Diaies Tonit <1 1.] 1.3 . 0062466272 008165257 0007922242 0.001343653 0002135572 5. 18 T¢a*ncnz vs D Control Lin.ar lnarpalaoon (200 Resampl.) Pomin % SD 95% CL(Eap) Skew IC05" 6.475 6 109 3 285 39169 2 0965 IC10 28 093 ICI 5 >100 10 IC20 4300 0.0a 1C25 >100 ICO )100 O8
'I50
>107
,1SiC es/aae less thai the lowesi ¢o4U/01ilfl n 06 "05 gos 0.4
- 0. 3 0.2 C2 0.1 00 0 20 '0 60 80 too 120 Dose V.
fDose-16p'oniar ]sIo
-- :al, 005 /evel 07 02 0.1 0i
.,q,,Il I06d--,:.
14-
xort-i-rea tea November 14-21, 2006 Statistical Analyses L-1v.I Fish Growth -nd Sr"i-I Test-7 Day Craow-.h Start Dat,, I/14.2006 Test ID ?pFRCR Samfle MD. "T]-A/ Seq oyall Nulvcal P1ait -n .0kc Eid Date 11/2112006 Lab ID ETS.Envt Testin Sol Sanple "ypc. DN7R.Dlschigr Noniloii:g Repoil Sample Dalel PlotocoL W'CHIR.EPA.821-P.02-013 Test Species PPomephales pomelas C(eiev,5s Nort-i"lead Conc.*I* 2 3 4 D Coomf'l 0 5 150 0 7320 07350 0.7790 100 0 6740 0.6040 0 7420 0,6789 Tiane/orees: f - n ne/oreted I-Taled Isoenic Con./-% Mean - Mean Olin NI- C079 N SL.,I Criu-al MSD NlMan N-M man D Corocl 07650 1 0000 07650 07320 0 8150 5 151 4 0 7650 1 0000
'100 06620 0 8654 06620 . 0.6040 07420 9 174 4 2.46 .9-3 00703 06620 0.8654 Au l*a, Teat, Suta s*( CrireI Sine. Kuen Shapao-WOrs Tts! od6cales coormal disu0bution .p > 0 01) - 0923090458 0 749 06143e1412 .09232.318 F.Tcs/ lidjcates equal vlas0/ccs (P = 0.50) 2375268049 47 46722794 Hypo1.h3ij T.a/ (I tail, 0.05) N- SDn MSDp MSB ,ISE F-Frob dlf Homosc edaslij ( Tto d, lces/tn ,dic4 d(ldonnc -,
C " 0070335791 0091942211 0021218 0.00262033) 0029350473 1,6 Ttamceoz is D -Coonol , ' " Lnme., Incte'polabon (200 "t".emple.o) Point V/. " SD 950,S CL(Eap) Sknw 1C05" 37 136
- CIO, 74272
[C15
>100 0 1C20 >100 .IC25 >100 ICO40 >I00 IC50 >100 0.7 " aodialrs IC esin:,te less 0*ha die J*o*sc1 coicertra/0*i/o 106 005 04 03 0.2 1
0.C 0 20 - 40 00 -00 1-7: 120 Dose % Doee-1*lepocen Plot 00 0.0 0ý7 .... .. ... 1 -ia,1. 0.05 level of sgnifcance 0.6 i 0.5 t7
*o,,
0.3 02 01
,qn _ 6/.14.06doco
Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimnephalespromelas Daily Chemical Analyses Project number: 2878 Reviewved býý, , Concentration Parameter Day (1 Day 1 Day 2 IJay 3 Da y 4 )ay 5 Day 6 hnltial I Final Initial J Final Initial I MnAI IniFinal h-iti FItna ial Initial Final Initial I 'i 41 (SU) 7.67 7 58 781 .7 45 7.52 7T08 755 7.08 7 77 7 26 7.81 7 43 7 .1 DO (mgIL) 81 7.9 8 2 7.7 7.8
- 7.0 7.7 73 77 7 9 7 8 74 8.0 Conductivty (tmhlios/cm) 314 320 308 315 316 3092 Control Akaluinity (mgJ- CatCO) 59 57 59 60
[Hardness (mg/I. CaCO1 ) 93 93 91 " 93 "lerperature(°C) 24 6 24 772 242 24 7 24.4 24.7 24.4 24 8 . 24.5 24 8 24 4 24.6 J1AIN) 7o 7 53 7.77 7.44 7 53 7 06 7 38 7.09 7 26 7.18 7 48 7 43 7.76 DO (mg/L) ' 80 7.9 8 1 7.6 7.9 6 7 79 7.2 79 6 8 7 9 7 4 8.0 Conductivityýnrihos/cin)
- 299 295 306 n 289 293 291 292 TVemperanure. (C) 24 6 24.2 24 7 24.2 24 7 24 7 24.8 24 8 24 8 24.4 24 8 24 2 24 7 HII(SU) 7.66 7,53 7.76 7.41 754 7.01 7,38 7.05 7 27 7 18 7 48 7 44 7.77 22.6% D 3O(rng/L) 8.0 79 8 1 7.6 8.0 6 6 8.0 7 2 7 9 6 5 7 9 74 .
ond u ctivil ,(jt ho s/ct n) 285 2 296 273 278 Temperature (°C) 278 . 278 247 24.1 24 8 24.2 24,7 24 5 24 R 24 6 249 24.4 24 8 24 1 24.8 p11(SU) 7.66 7 56 7 74 7.40 7.53 7 04 7.30 7.09 7 26 7.19 747 7 44 7 75 D.)O(mg/L) 8.0 79 8. 1 7 5 8 1 67 8,0 7.2 79 65 80 73 , 1 45.2 Conducvit, (pmhos/c) 257 258M 246 251 252 250 Temperature CC) 24 7 24 2 24 8 24.3 24 9 24 5 24 8 24.5 24 9 244 4 1 24.3 24 8 (51)) Ull 7 65 7 56 7 73 7.401 7 52 7 081 7 34 .712 7.24 7 18 745 7.42 773 72.6% DO(mgiL) 1 . 8.1 7.5
. 80" 69 80 7.3 80 6.7 8 7.4 82 Conductivity (ptinhos/cm) 222 223 231 211 217 213 217 Temperature ('C) - 24.9 24 2 24.8 24 1 24 9 24.5 24 8 24 8 24.9 24.3 24.8 24.2 248 pHt (SU1) 7 64 .7.54 7.71 7 42 7 S1 DO QnO,) 7 06 7.33 7.07 7 22 7 181 7 43 7 46 7 701 8.0 8.0 8 2 7.5 7.9 68 82 7.3 82 666 8.2 72 8 3 CoOMILuCiVity (J/rnIMS/CITn) 1881619818 182 182t18 (10%Ii Al~kainity~i~(ig/l- Ca,:CO 1 ) 66 6567M I'Lardniess (mIg/L C C'Oj) 77 79 81 Total Residual Chlorine (mgJ.) <0.10 <0.10 0 10 Temperaiture CC) .25.1 - 4 41 24.8 24 3 25.01 24 5f 25 0 24.6 25.1 24 4 24.1 24 2) 24.8 pi (SU) 765 755 77.1 7.41 .751 7.09 7.30 7.0 7.20 7(7 .41 743 769 DO (mI..,, 80 8.0 831 7.5 7.8 7.1 8.3F 7.51 8.2) 66127 .
Conductivity (11'ithos/Cm1) . 184 16 18IO82 8 179ý 7 - 183 100%/ Intake Alkalinity (mg/L CaCO 1 ) 64 66 66
-Iardness (ngiL CaCO4) 79 77 77 Iotal Residual Chlorine (nigL) -,:0.10 <0.10 K<. 10
_Temperature (°C) 24 9 24.1 248 243 248 24.6 24.9 246 248 243 2477 24.3 24 7
Pa- I.. of6 Environmental Testing Solutions, Inc. Species: Pimephnlaesprom elas Client: TVA / Sequoyah Nuclear Plant -Non-tremted Daily Chemistry. Day, Parameter ;ystl= rna -] Concentration CONTROL pH !s*S U-q- "g _ 1 ".2 qj DO (me .L) a Conduct ivi I (umhos/1cm)' 3Z (mag aCOý/L). 6-H (mardness * "" ------- CaCO 3 /L) Temperature('C) I ,A Z Z.A. -1 ii 2z. 2 . .- I - 4.
"-1 11 .3%/ DO£ PH(SU)
(SU.L)
' q ý/ 45.I q "q.L4
' L4.Lt_
'q
; lf Conductivity 11.60/.
(" mhqos/cm ) DO (mngfL). Temperature ('C) 2 L q._.-'-____-.- kt
,.> .2
&O
-I.,-
r 22.6%/o DO (mgJL) ___. __-__- ((pmhos/cm) (.2.. _____.____..Temperature(_ - _ _ 2q. z1.S
*H (S.U.)
Conductivity 5 -,; -0c 9.-,O I-4
*0:}q 47.6%
5 DO (mggL). Conductivity ( mhos/cm) _ _ _ _ _ _______-- TH ( U.)'a. e°C 2"4.2- * .* -O 72.6% DO (mg!L) Temperature ( CQ 0 2LI1 AA2-I e,
- 2. 2A -L,4, _
(q.0 LA_-_S (Hmhosicm) 23 Q Temperature (°C) q " - - 2 Z .\ i Z * - 2q. Conductivity
]oooH (SU. b.4 "4 ** ~
Alkalinity (mg CaCO;/L) Hardness (mg CaCOCJ)" TR chlorine ID4o 1 (mg/L) C) <0, Temperature ('C) . 2q.q 2', .7 1.. ___._ _.. pH (S.U). - -l q0 j .1 1 100% Intake DO (mL Conductivity
* .0 83
(,mhos/cm) 1 SQ 1 OtS Alkalinity (m CaCOv'L) Hardness (mg CaCO/l.) TR ch'lorine (mg/L) Temperature ('C) 7Q-. 2,1 A. " 2 . 4 2q Initiat II Final I Initial Final I nitial
Environ mental j e~s't .,'lU Ullý4l Speces: Pimeph ales promehts Client: TVA / Sequoy'ah Nuclear Plant - Non-treated
.7 V 3 4
'7 .7 . V -
Analyst moý, Parameter *
" -- - -. .. 3" - ' .
Concren-tal 1 On I _ , I I . a CONTROL pH (S.U.) r4.-5% I1 Q06 I i I I- - t 1
. - T/ýA/ r DO (mgIL)
I '- I 9.8 Conductivity 3(p e' (urmhos/cm) Alkalinity (mrg CaCO;/L) 5q Lqo Hardness (mg CaCO:/L) Temperat'ure_("C) - 1 1-k.4* Is." -_ ,-\.6 .'. - .. PH-t (S i.) ___.___ _g.I_ ... 11.3% DO (mg'L) -D9.2 ____._. _.____ Conductivity *, (,imhos/cm) Temperature (°C) 2 3 _ _I_9_c__2 q r J 7. : -~. LA.-, Z'4.1z /q -. 1q-) "1,. pH(S.U) -O6 . q3,G- ! 9.-I ".T1 9.' 22.6% DO (m&L) .______._______ Conductivity _, _ _._2 _ ,_____ ._,_6 (pmhos/cm) _ Temperature (°C) 14A 114 A0 .3 !l l 2.4.i J -R- k. Lq. A-pH (SU,)_-_"- _ 9.2c.q z C- 14 - .4qL " t. 45.2%/ DO (mg/L) -0 "4. z- © .". ." C ond uctivity, '4 (. Temperature ('C) 4.11A "e _"-. 21qS' . ___ _, pH (S U.) Z 2.- ~;- 4 2 93 q.. 0Pj~ 72.6% ' DO (rmg/L) e.O _-2 6___ Conductivity 1 4 (pmhos/cm))______-_- ,______,__- pH3 Temperature ('C) -1.4 ' 2M. iS+°. 1
+Z 2 ,- _",. _ _ 2q
.'4 62- _
"}.6z)
.13 100%/. DO (mg[L) .. 0.a Conductivity s eZ (nrnhos/cm)
Alkalinity Hardness TR Chlorine (mg!L z 0[j[1OI a i** i
,'Temperature (,C) ". ! ,I.l-IA 21.J 7.--- -- q.p' 100%o D O (m g/L ) -" t-o.t. . "" . " - ,
Intake (Umhos/cm) /80 " (mg CaCO3[L) *- TR chlorine (rneL' 4 -10 _ _____ Tern erature (0C 9 ZLI zq.~R ?4 2 z .4 I Final I Initial I Final I In iti.31
Lnvironmental Iesting2 otons, Inc. Chronic Whole Effluent Toxicir: Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadub/a Client: TVA County: Hamilton Facilit)y: Sequoyah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project 9: Test organism information: Test information:. [Orga~nism _age:.- Rabdomizinc tem~platc: csge Date and times organisms . 1\-v'5-o" ' - "ook Incubator number and were born between: shelf location: Organism Source Cups: 2t ,Z16,*o " YCT batch: - Culture board: iV-cn-ok ,* J -!> L, ,4l4,1k Transfer bowl informationi pH 1.12. Temperature =".q Selerrasirun* batch. 1 i.-C' Daily renewal information Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time ____ _______ I\ 7Z'i 1\l-1-0I Go 4t-10 6 1*\-20-O* 11 2.2- -IS-"OG6 10 ,t\t11.oc '4-10 Control information: 1 2 Accepiance criteria Summa oftest endpoints: % of Male Adults: 0:) C7. s_20% 7-day LC50 > Icfl7. % Adults having 3 A Broods: JcOo' - ,0o7. >80% NOEC I007, % Mottality: 01 n*. s 20% LOEC 10 0"7ý Mean Offspring/Female: Z.-9 2, >_.15.0 offspring/female ChV > IO.o7/,. %CV: (il.1 ..Zo1 <400% IC25 > tool.
Environmental Testing Solutibns, Inc. Pag!e 2 o1 / Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant*- Non-treated Date: t\-tA-c,* CONTROL - Survival and Reproduction Data Replicate number Dav 1 2 3 4j5 1617 j 7F 9 10 1 Young produced O_ c_ : c 0 0'_____ Adult mortality ( - tj - I-. L . 21 Young produced 1 oF ) 1o ao oC)~0 Adult mortalitr y. 3 Young produced cff 01I! 01 Q. 0 c (.'I) CD
...._ 05 Adult mortality _ .__ L ____
- 4 T Young produced -3 -S-A L"A2 '-4 30 3 4." A{s 0 if-5 I Adulhmortality Young produced (LV DT=ead 1jQ0 /L 1'tv.
inlAdult mOr talir y Adult mortalryt 1 ",-1 &I,.. L._ ' 1,.._ k..- I ... I........
- k.... .
6 1.oungproduced 0 C)1 F0 an eprodtio DaT1 P30 6 [Youg oI poduedlConentation [o z1 8 9 0 Total young produced _ ' O0 2- 2_ X for 3" Broods u "?4. __ __ > . , . Note: Adult mortality (L= live, D dead) Concentration:
% Mortality: )7.
Mean OffsprmingFemale: z R.'" Fia Adult mort N_.ality k._.
- QL _
coc 11.3% Survival and Reproduction Data
}
__________ _________ _________ Replicate number _______ I Young produce-d 2 : 0- 0 j 0_0_Do_ DA dul _ 6 7 81, Adult mortality 2 Young produced _ _ _ _ _ _ _ LA~dult mortaltry - 4 Young produced If F _ c I ~ ~ ~ ~ ~ f Ault Mortality A_ _L-[L I__ __ _ _ 6 Young produced f__ c_ _ _o__ Adult mortality 1
- 1 F 7 Y'oung produced Total young pr~oduced j F_3 I'- t.j I (
~ 1 3 '
I I.
~ f~
.L Final Adult Nfortalir- -\.., _ _ _ _ __ - '
Note: Adult mortality (L lvc, D =d-rad) Concentration: Mean Offspring/Female' o-
% Reduction from Control: -6.-7
Environmental Testing Solutions, Inc. Pace 3 of7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: 11-(t4 -Co CONC: 22.6% Survival and Reproduction Data Replicate number Day I 1 2 3 4 f S 6 , I 9 10 Young produced 0 I Q 0 "COfflO c 2 _ [ Adult mortality Y*oung produced Adultmcrtaliry j_ _ _j o- o) L
~ i C)
'-I 10 L
'-~_
f _ _ _ 0
.3 oung produced } 3 ,= L. i o 0. I { ,.0 0 f 0 e Adult mortality producedn__ t.Young Vti t\ on."
I N1 (1/\ 1[_Young produced Fnl Adul itmor ta*li ty, _ _ ____[___
* , ._...
- I..-k
- [ L
)i~
A dult mortalityt i 7 1 Young producedO n FeL e: 1 2 Total youn.g produced. uria nl Rero 3S 31io 30 Final Adult mVortali t y 1,.__ .. ( 1. \ J . Note: Adult mo rtality(L live, dead) Sl ~ ': tConcentration: yoot M'ean Offspring/Female: 3 Z.,
%Reduction -from Conrrol; 5-7 20 Go.NGca .4 5. /% Survival and Reproduction Data I
Day _ _ _ _ _f I Adult~~~~ mortality 2 3 4 _ -Replicate S number 6 {._. 7-8 9I 6-.7 10 Adult mortality h t..
- ____ ____
21 Young produced O[ TojD c oI o1 o
"°1Xoug rodce Ad ult mortality Q*- . l1
_ .. _) " Q" C . 0tl
'QOJ 0 0 _ ,_
___ Adult mortality __ _ _ .- I 7 1 Y J: ____ 4 Young produced A'_"-1 A sTL 41, V V717 LAd ult mortality j L U Youn ig produced . . ,. 1,. Adul t mortality Yugproduced -A 1_ 0~ (0t G J3 6 _0_
.7 Young produced 1'1 0' ) 1'-1 1 o jj Total young pro duced 2Iq 3 0 Final Adult rylortalitv \ \. -_ ___ i Note: Adult mortality (L = live, D = dead)
Concentration:
% Ofsortalinty:(3 Mean OffspringfFemale: 3ry .o
% Reduction from Control. - 2'1
Environmental Testing Solutions, Inc. rag- , 4 ' ,' Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Da I7-11: -k (o:\ 72.6ca Survival and Reproduction Data Replicate number Dav _ .1 2 _3 4 5 6 7 8 9110 Young produced O) c) (i L C O __CnO Adult mortality ,___ j ,.. K -- .- 3 Youngproduced , /'.Tl c,' c) Adult mortality _ j _ _._ o* .. T ___ 4 YYoung produced '-A ____ S Young produced £*2 "_ ___ I L_ _ '" ___ -_. AdAult mortality na Adultmortalit y f L . L L
.o [
L . iI L I I i_. I 1._. I I._. k_ 6 kYo ung produced _____ _____ ___D_ ____ 0 __ AAdult mortaltry 7j ýYoung produced 0 & t~' ' ~ 1I~i Total young produced ~t ' 32- 3' 3~ Note: Adult mortality (L h.e, D =dead) Concentration.
% Mortaliiy: 0 o.
Mean Offsprng/Female: 33.2
% Reduction from Control - 1 .
0 Surv'val and Reproduction Data CONC: JOO % Replicate number Day 1 2 3 4 1 5 6 7 8 9 10 I Youtng produced ( 0 0 ( 0 o L 0 _ _ Adult mortality _ _.. _ l..___ t_, t,_ __ _ - 2 Young produced ]*1 t ]f O c'< c_ OJOTO Adult mortality , L Adult mortaliry FL__ ~I' v"~---_ --- b- LI_. '-1_ -I,.-- 6j _ Young A_ produced~
.dult mortality } _ _ ____ _ Ji__.
_ t] tJ_ '_. 0A~ -I _ICVII) L__I.- L_ - --L_ _ 7 Young produced C '50 , I i6* 11 Vi ' T i9 Total young produced 6 a, I _1'_ [ )31.3 S 3. Final Adult M1ortaliry ___ __ _____K L Note: Adult monaliry (L =li,.e, D = dead) Concentration:
% Mortaliry .
Mean Offspring/Female: ,-{. 2.7
%/o Reduction from Control: -I. 37,
Environmental Testing Solutions, Inc Species- Ceriodaphnicadubin Client: Sequoyah Nuclear Plant - Non-treated Date: tI - CONTROL - 2 Survival and Reproduction Data Replicate nurnber Day 2 f3 5 -. L 6 1 7 8I 9 1,oung produced o o CDo .o 1 c o o A dult m ortality *.. . ._ 2 [Y-A~"'oung produced 0) C) ~ 0i F C)o~ Adult mortality . 1 L_-* , 1F . T oung produced C)I 0i 1-1 C) c 4 Adult mortaliry Young produced, (*U '- -L
'-ý- E ý q "- _ C4J' ,..-- ~ L__ ' Vc)~ v L k.Idt mortality ._ '- - , . I
"__ .._ _ J _
7 Young produced . ,1 ý1, 13 1Q-Total young produced 29 " C- 2.9 30 2.9 Z- R I 2-1 3.- Final Adult NM ortaliry L. -. .. t. -.. . Note: Adult mortality (L live, D = dead) Concentration:
% Mortaliry: 07.
Mean Offsprng,/Female: 2z9 - i CONC: 100% Intake Survival and Reproduction Data Replicate number Day I Young produced 1 C) 2 01 3 C.j 4 ]* 5 6 010 c 7 - C 8 9 10 [~~~~~~:t-oru: aty% Adult mortality Youngproduced Io C__ __ I 1 o:I%
.-...- I C )F l* t* I %
j _ C o c1 - 3 Young produced J t t I 0 IJ- *I
- I I I I ,
Adult mortality, . __ _ k_.._ __ _ _ _ __ ___ _ . .__ _ ..__ __..__ k,.___ _ . 7 6 You ng p r oduced Adult mortality Adult mortality Foa [Young produced young produced JJ3Fi__
. [IL.
I .. FS ii'-s
~
I
-I
~o
~~ ~ ~ qFl oli
_____S3 L- ____*3S sI L. Note: Adultmortality (L libe. D = dead) Concentration:
% Mortality: 0"I IMean Offspring/Female: y5. 0
% Reduction from Conrrol: - S.
-'VA / Sequoyah tNucleiar k'Ilat, Outiall 101 Nort-trea(ed November 14-21, 2006 Verification of Ceriodaphnia Reproduction Totals Control-] 72.6%
Day Replicate nun~rr RpccnubrTotal oa t ~Repc(jtiuaenurter S 3 4 57 8 Day '1 2 3 4
,1 5 6 17 8 9 10 Total 9 1n 1 __ 0 0 0 0 0 o 0 o 0o 0 0 0 0 '0 0o 0 0 F 1 0 2 0 00 0 o o 0 2 0 0 0 0 o0 o 0 0 0 0 0 3 0 0 0 0 0 o o 0 o 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 4 4 4 4 41 4 4 4 5 4 5 5 5 5 4 6 .47 5 10 10 9 10 9 9 11 10 13 11 102 5 It 14 10 14 II 11 15 10 12 13 121 6 0 0 0 0 0 0 0 0 0 0 0 6 16 0 16 0 0 0. 0 0 0 0 32 7 15 13 13 16 14 17 15 13 15 15 .146 7 0 19 0 18 18 16 15 18 19 15 138 Total .29 27 27 30 27 30 30 27 32 30 289 Total 31 37 .1 36 34 32 35 33 35 34 33M 11.3% 100%
Day ltepicate nUmbi - Total 1R. licale ntiwi~er 2 3 4 5. T 6 7 8 9 10 Day -f-- ---- - - - - - Total 1 2 3 4 5 6 7 8 9 10 1 -0 0 0 0 0 0 0 II 0 0 1 0 0 0 0 0 0 0 o 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 , 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 3 5 4 S 4 4 4 s 44 4 4 5 4 6 5 5 4 5 5 5 4i 5 10 1. 10 12 10 10 I1 12 11 11 1 llS 5 .12 13 II 12 10 14 I1 14 14 12 123 6 0 0 0 0 0 J 0 0 0 0 0 6 16 0 18 0 0 0 0 0 0 0 34 7 14 14 16 14 17 18 16 13 15 18 155 7 0 15 0 18 18 1.7 19 14 19 17 137 Total 28 29. 31 31 31 33 31 29 30 34 307 Total 32 33 33 36 33 36 34 33 38H 34 742 22.6 %/o Control-2 Day Rq1 itc tiutber Total Day Replicate ntutijert Total I 2 3 1 4 5 6 7 8 1 .9 10 1 2 3 4 S 6 1 7 8 9 10 00 0 0 0 0 0 o 0 1 I 0 0 0 0 o 0 o 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 (0 4 4 4 5 3 4 5 5 5 4 4 43 4 4 4 4 4 5 4 4 "3 4 4 40 S 13 13 10 11 11 10 11 13 12 10 114 5 10 9 9 9 11 10 10 1 to
. 12 101 6 3 0 0 0 0 0 0 0 0 0 3 6 0 0 0 0 11 0 0 0 0 o 0 '7 13 17 17 14 16 16 19 18 19 16 165 7 15 13 17 16 14 15 14 17 13 16 151S Total 33 34 32 28 31 31 35 36 35 30 325 '02otal 29 26 30 29 30 29 28 37 27 32 291 45.2%
100% Intake Day 2ay 17 2 3 4 RcpbcAt 5 nu6 ber 7 1 8 9
- 10 .Total Day H hTotal 1 0 11 0 0o 0 0 0 0 0 0 II I 2 3 4 31 "7 38 9 3o 2 0 0 0 0 0 0 0 0 0 0 0 1 o o o) o 0 o o1 o o o 0 2 0 0 0 o 0 0 o o o o o o0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 5 4 5 4 4 4 44 4 4 4 5 5 s 4 4 4 5 4 44 S 14 II II I1 10 12 J2 13 10 11 115 5 15 11 11 11 10 14 12, 13 1.0 12 9 6 2 0 0 15 0 0 0. 0 16 0 33 6 0 o 0 0 0 0 17 0 0 o0 !
7 18 10 17 "0 19 16 17 14 0 19 138 7 19 15 18 18 16 15 0 19 18 18 5 93 33 133 31 1 34 32.L 34. 31 30 34 330 01I Toi 38 30 34 34 31 31 33 '36 .3 [ 34 33
i v ,v -)cquoya n INuCIu YI
-idl L,JUL~d IdI-JI Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2878 Reveisoed by: r, v/ s Concentration Replicate number Survival Average reproduction Corffici'sit of Percent reduction 10 ("/-) (offspring/fem ale) carial io n (n .) pooled controls I 2 3 4 5 6 7 8 9 (I1 Control - 1 29 27 27 30 27 30 30 27 32 30. 100 28.9 6.2 Not applicabl 11.30/, 28 29 31 31 31 33 31 29 30 34 100 30.7 6.0 -6.2 22.6% / 33 34 32 28 31 31 35 36 35 30 100 32.5 7.8 -12.5 45.2% 38 33 33 31 34 32 34 31 30 34 100 33.0 6.9 -14.2 72.6% 31 37 31 36 34 32 35 33 35 34 100 33.8 6.0 -17.0 1001/6 32 33 33 36 33 36 34 33 38 34 100 34.2 5.5 -18.3 Control - 2 29 26 30 29 30 29 28 31 27 32 100 29.1 6.2 Not applicah 100°/,, Intake 38 30 34 34 31 33 33 36 33 34 100 33.6 6.8 -15.5 Outfall 101: MSD -- Minimum Significant Difference Dunnett's MSD value: 2.123 PMSD = Percent Minimum Significant Difference PM SD: 7.3 PMSD is a measure of test precision 'The PMSD is the minimum percent difference betweme the control and trcatment that can be declared statistical.ly significant it a whole effluent toxicity test. Oni verage, a signtficant diffeiet'cc occurs- for Environmental Testing Solutions. Inc chronic toxicity tests w Intake: a toxicant reduces Ceriodaphrmnin reproduction by 96% frot the control Dunnelt's MSI) value: 1.586 L.owei tMSD bound dctcrrnincd by USEPA ( 10 h percentilc) I PMSD: 5.5 Upper PMSD bound determined by USEPA ( 9 0 "hpercentile) 137% The lower and upper bounds were calculated by the USEPA usifig 393 tests conducted from. 33 laboratories for Ceriodophnin ieproduction in chronic. reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protectinn Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Statistical Analyses Ctriodtphnii Survi4,l and Reproduction Tut-Reproducoon S0anrlDatl: 11412006 Test ID CdCFCR Sanpln ID T0A Oquoyah Nttciean Plant Sc - Oulfall /0 End Datl 11!2112006 Lab ID ETO-OS E-4 Tesling Sol San~pl. Type. DMR-D*sh*og Monitormg Report S-mple Date . toDoOl FWCHR.EPA 821 R.02.013 T.! Speces CD-Cmtodophnta duIbia Colmnenls Non rnoted Cone-% 2 .1 4 4 6 7 8 y 10 D.Conbol 29000 27000 27.000 30000 27000 0.000 30000 27000 32000 30000 11 3 23 000 29 000 31 000 3!.000 3 /000 33.000 63 000 29 000 30000 34 000 22 6 33 000 3a000 72 000 23 000 31 000 31 000 35 000 36000 35 000 30.000 4 2 38 000 33.000 33.000 31 000 34 000 32GO0 34 000 31 00 30000 34.000 72 6 3/1 000 37.000 3/ 000 36000 34 000 32000 3C000 33 000 35.000 34 C`s/
/00 32 C00, 33.000 33 000 36 000 33.000 36.000 34 000 33 000 33 000 34000 Transform: U:ns-stormed -T.TiId Iso"onic Co-c-%/ Mean N-,tin Mi-n M i, M-e CV' p6 ..t6-b Crrtickl MISD M an N-.Mrn D-Cotuol 23 900 / 0000 28 900 27 000 32 00 6.201 /0 32.13 / 0000.
/1 3 30700 .0623 30700 23800 34000 5957 10 -/ 939 2.287 2 /23 32 /3 /0000 226 32500 11246 32.300 23000 36070 7.343 10 .3 978 2267 2123 32183 1 0000 4S2 332000 1 /419 33.000 30070 38,000 6351 /0 .41/6 2287 2/23 32183 1 0000 72.6 7300
" 1696 )) o30 31.002 3700" 6047 /0 .5 278 2 2Y7 2 /2) 32/ 3 0000 i00 34.200 131/34 34200 32000 3303 0 5479 /0 .5709 2287 2 123 32/13 /0000 AuxilItry Te t S t4i ti, Criti-a Skic. Ku"o Kolrtmgooc DIlest MKssedalnormal dLibutt, (p > 0,01) 0 736118853 / 035 0.2673 12653 .0 34924*3 1 Bait/hWs TrI indiscatssequal v,-iancn (p =0 R8) 1 74052 1503627224 3
l'poaeo*o To.t1/3-il, 0.05) NOEC LOEC CsV TU NISDu fSDp MSB MSE F-Psob- df DOusett s Test -. I(DO 00 1 2 122349295 0073454993 40 85666667 4 309M2059 16L-06 7. 54 TsoaorntL,0vs D.Contiol Lintr Invrpo/taon (200 Resmpsle) Point % SD 95% CL Skew ICO5 >100 ICIo >100 IC/S >100 to IC20 > j(0 9, /C2 /
>10. 809 lC.O >100 0.7 IC5O '100 0.6 0.3 02 0
0.1
&0
-0. 7 . ... . . ." . . . . . . . ..
-0
-0.3 0 20 40 60 20 100 120 Dose /
Dos e-Rsponte Pl/o 40 I-tail 0.05 level 25 0c of significance 20 o5 10 0
.tqsol/./<-O6dolo
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 14-21, 2006 Statistical Analyses Cerioda phnia S-rviV A)ad ReproduaUon Ttst-Reprod uc ton Stai Date. 11/14/2006 T'st ID CdFRCR Sarnp), ID F A 3 Seqbyn , >>ckla. t .11 2a3k End Da, 111,2212006 Lab ID FT5.0E- T7slongSol Sarnpl, Type DMR D6cIh& Moflot-ng Rtpoll SamnClDate Protocol FWCHR.EPA.821-R-02-013 7est1Spncs CD-CmriodapLa dub. Cornmen'3 Non.teated Co.n-% 1 2 3 4 5 6 7 8 9 10 3.Conlnol 29000 26000 30 000 29000 30 000 2900 28 000 31 000 27 000 32,000
)O0 38000 30000 J4000 34.000 31000 3300 3000 3oo 30000 33000 34000 Transforin: (Jnsrbnsformed I-Tailed Isotern Con-/ M-eon N-in -an M.nl Mrn M .t Cv/ N tS.1 Crio-a MSDI SDan. N-bIn D.Conno) 29 100 CID I M 29 100 26000 32000 60 51 10 31350 13000 300 63600 131546 33600 30000 66000 6758 30 4920 13734 5B6 3 )0 350 1 0000 A-oili3.ry Tea> Stan,6, C6i6-al Sh1n . Kurnl Shap-o-WU,4s Tes, ndad oes i>or-al dlotbufion p > 0.01) 0.973339081 0 660 197a06397 0234273437 FTeist bbd-(ae', q,.a atcs (p 0 49) . 3 605536342 6 534080535 tvpoO.t,,s Tst (l-Lol 0.005) MInlSD. MSIOp MSB N1SE F-Prob df Horoscedwhc I Tent mdicatcs no significaml dfTrdccts 3 566139066 0034506497 303.27 4 363333333 1.31.00 3,IF T,,amn~cnu s D.Con-tol Linear Intotpohttion (200 R-mmplu)
Point % SD 95S CL Skean IC05 >100
- CIO >100 ICI5 >300 1'0 IC20 >100
- 0.
1C23 >100 0-" ICO >100 0.7 IC50 >300 C 04a 002
~0,2 0.1 00
-02 . .
.0
-021 . . . ...
0 20 a0 60 70 100 120 Dos..n 3-oa0 05 leve; c2 of sigbuicance o23 10 30 04
.tqn _11-I 4-O0dato
JL v t-i. / OUqUVYdIItyi L1LlUdI JL dIlL, 'JULIdIll II1 Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia D)aily Chemical Analyses Project number: 2878 Reviewed by: Concentration Parameter " Day 0 Day I Day 2 Day 3 1)a 4 Da 5 ' Da 6 Initial Final Initial Final Initial Final Initial Final I nitial . Final Initial Final Initial Fina 7.61) 7.71 7.81 7048 7,52 7.34 7.551 7.27 7 77 7 51 7,81 7 67 7.81 DO (mg/L) 8A1 83 8.21 7.7 7.8 76 7.71 8821 771 7.9 7.8 7.9 8.0 Conductivity (nrnhos/cm) 314 Control Alkalinity (ug/i. CaCO,) 30 312
.59 576 Hardness (mg/I CaCOj) 93 93 91 93 Temperature (C) 24.7 25.1 24 91 24.6 " 24.6 25.2 24.8 24.9 24 7 25 1 24 81 249 24.6 2 (U) 7PH
.765 7.69 - 777 7.46 7.53 7.34 7.38 7.27 ' 7.26 7.51 7.48 7.6 7.76 7 11.%IDO gL)
Conductivity (1mhos/cm) ._299. 8.0 8.3 80 295 :7.7 7.91 7.6 27,91 8 0 7.9 7.X 7T9 7S 8.l0 28629 291 292 Tepeatre..2.8 27 T peareC)24.71 24.8 24.8 24.8
- 2. 481 24 9 74 L2 7722.
. 24.9 24.7. 24.9 24 9 24 7 24792 PH (-SU) 7.66 7.67 7.761 7.48 7.54 7.35 7.38 727 7 271 7.52 74X 7.691 7.77 DO 226 8.01 8.3 8 Ij 7.7 8.0 7.6 8.0 7.8 7 9" 7.71 79 7.8 8.1 22/n Conductivity (jimhos/cm) 216 6 273 278 27 S 278 Temperature (C) 247 24. 24.8 24.6 24.7 24,7 24.9 24 9 25 5 249 252 . 4.
pH (SU) 7.66 7.67 7.74 7.48 7.53 7356 7.34 7.28 7.26 751 7,47 7 68 7.7S 45.2%/o DO (m g/L) 8.0 83 8.1 78 8 . 7.6 8.0 7.81 78. 7 9 88 7098 1 Conductivity (l1 imhns/cm) 223'23 258 2652"' 246 252 250 TemperatureC) 24. 1 24. 248 24.9 24.7 25.1 25.0 24 9 25.0 25.0 245. 25.1 24.8 I tI) 7.65 7.66 773 7,47 7.52 7.38 7.34 7 31 724 7 50 7.45 7769 7 70 DO (ng/L) 8.1 8.3 81 7.8 8.0 7.6 8.0 7.8 8.0 79 8 2 7.9 8.2 Conductivity (p mh osl/cm) 222 887 223 23 1 Temperature( ('Q 211 28 1 217 24.8 .24,S 24 8
- 24 S 24,8 25 1 25,01 24 91 25 2 25 01 25,0 2-5.1 2481 100t/o Alkalinity (mtg/l. CaC( 1 ) 66 65 67 flard.ness (mg./L CaCO 3 ) . 79 810 Total Residual Chlorine m 7010 -/L <0.10 . <0.10 Temperature (C) 25.2 25.0 24 7 24.8 24.9 24.9 25.2 24.7 25.3 25.1 25.0 24 7 24 8 II SlI 7.65 7.67 7,71 7 48 7 _1 DO (mg/l.s8.3 7.36 7.3090 770 8.3 7.7 7.8 .- 7- .9.. 8.3 - 7. 82
. 8.0 8.2 8. 84 Condluctivity ipnhlos/n 184 186 1918121713 100',' Intake Alkalinity (mg/I. CaCO,) 64666 H-,ardness (mg/L CaCO 3) 79 . 77 Total Residual Chlorine (mg/L) <010 .77 1
Temper3ture (oC) 25.01 24.8 248 24 8 249 24 9 25.1 24.9 249 251 24.91 219 '24 81
Species:. Ceriodaphin dubia Date: i L- c(", Client: Sequovah Nuclear Plant - Non-treated Dailv Chemisv}.
"Da y Pa ...rameter.. C)L toten COHTROL pH S.U 4.
Conductivvyit 3 (rng CaCO;/L) _pH (SU ) - .('5, 1c (0 q,5 143 Temperature(°C) -L"4 1-- " L." "-A- ""S 100 3% DO (rng, 0L)0 6-3 a A 10% DOrnigP) pH(.S.U) '-4.8.1 L.]19<d, ca.g 16 1621 -3,9.5-q 3 (rnhos/cm) 2,6/o2 DO (__ _mgfL) 0.0 - . Iq * .I ký Alkalinity (pmhos/cm)
- SC £ )
Conductivity (mn CaCO3/L) 752/.6 Temperature rDO (mg/L) (°C) e*.1 0{ " 77'L.o , ".{ 9.1.] 3- 7,q. ! (,mhos/cm) aodcivii r,y* npHiS.U) q In
*"S"*..
Lin.l F.nal I t Fina l Omhos/cm) -v1 Conductivity A6 18k" Temeraur ) (aC~ *", -, I 72.6 5D(m/L-) _*mos/cm) *q O Alkalinity Hardness -19 ' mgCaC04/L)"1f-3 TR chlorine IC (rag/L) LC /0 Temperature (°C) 7.5-o 11. * *q.-l
- t !z , 1 q, Alkaliniltiya Fna niia* Fi a
Lflvlrtnmental Testling SolutiOns, Inc. Species: Ceriodaphnia dubia Client: Seguovah Nuclear Plant - N0on-treated Date: I\- q-©. DaY. 34 6 Analyst IX"Y I Concen- Parameter .- i . ..
- i tration CONTROL 2H (S U.) 0
.DO(rn, /L) -!. r . ___ _ I.Z Conductivity (umhos,'cm) -
Alkalinity (rmg CaCO,/L) to Hardness (mg CaCO;/L) \ Temperature ('C) h.. '-LA. C "-L.4
- ' 1'45.
*.L, .\ "1.. . A, 8 PH (S.U.) o.* q** 9 c ./ 9 8 q* .- .
11.3% DO (mg/L) 6.0 6.0 Conducti itty, A9 A " _(mhos/cm) Temperature ('C) ".q F.. - .c, -L2- l . Z . " . 2 Zq * " L¶,. \ _pH(SU ) "*ý.--ýB q .Q 9-ý 9 .9- -.4* ' ,2Z__-1 8 q-,% 5 22.6% DO (mgL) S83.0 el .- 51 . 8.3 Conductivity rnmhos/crn.) 9, ~3 .'ý2 A Temperature ('C) .2z Aq 2A.s " . "'1.q. 9'. . . 'L. o pH (S.U.) 2-3C1 ý2 J4 q( 45.2%,,o DO (_n.'L) .. 9.* Conductivity (rmhoslcm) . A 0 Tec perature C) ::4* "z.. 2'.i .. e pH (S]U ,50 z L? 72.6o DO (m ,'L) .2D 6.0 Conductivity (u.mhos/cm).. Ternperature (1C) 7j. 0~. ZS. 7 q. If Ps pH(S U 23? a) q ~ C)"5 9-43 4-ýo* q 7 0o q 100%/ DO (ing'L)e Con ducti vity. ( rnhos/em) Alkalinity (mg CaCO;L). Hardness (mg CaCO;/L) IR Chlorine (ng/ L).1 Temperature(IC) 2S- "2- 1,--." 24:{ Le pH (S.U.) .. 92o 2..r0 . '. 100% DO (mrgL) .9.5 .-. 81 4 3 Intake Conductivity (pmhos/cm) I 0 l (mg CaCO3/) " Hardness "-7 (mng C a'CO0,, U TR chlorine (m ZO . I q 2 I
"! "q . . ". 1 .
Temperature ('C) Initial FiInit i Fnial l Final Initial Final
Linviruliumil-1 I t:3LIJILý )U-- ýI- -Th;c, I .IL 'U Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas
- Client: TVA County: Hamilton Facility: Sequovah Nuclear Plant Treatment: UV-treatedc NPDES #: TN 0026450 Outfall: 101 Project 4: 1.1 i Dilution preparationinformation: Comments.:
Dilution ptep (%) I 13 22.6 45 2 72.6 100 Each concentration was treated Effluent volume (mL) 282 5 1130 18 15 2500 for 2 minutes with a UV sterilizer Dilucnt volume (mL) 2217.5 1370 685 0. to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism informationf -z-t.2$ ý-LS Test information.: Organism age: -e,,-a v ..S oil> Randomizing template: 4&u e. Date and times organisms -i"-A00 o'o 160o Incubator number: were born between: Organism source: rk6% P,-rck ( \-11\r.O, Arremia lot number: 70"4 , Transfer bowl information: pH = Temperature 0C Total drying time: 2. ' 2A . Date/Time in: ti o0 o*,*t Average transfer volume: g Date / Time out: ll-'22d--- IL4 00 Oven temperature: (,0 'C. Dailyfeeding and renewal information: Day Date Morning Afternoon Test initiation, M'IHS Sample numbers Analyst feeding feeding time renewal, or batch used used time -termination time 0- (CO-10 I-2c~ t~ ~Z 01 1 !\.L100t OO IkoO% l' "-vL I \--S< 1 t- 0'1* O C)/$ z-7 2 1tt-- . t I c C.-I '0 . 1\ -r L-Ot_ 0
- iokkNN_3 3 I1"t-I-O \-, i oc )-"b 'it- %k"b ,q t~o I\\"Is "o r° P*" Cr
)"\ "" lt
___ *O~, 11 IOZ I toC)S A,_____~tr, 0(~~. 1c\ 0 5 It_l`)i 0L Ocl'a' I 00,oO- \',-.Or,,6 r *\ . Oý o < 6 IC`lO1 0:1 -- 0c) ,-ot,* I- 3 i ,, . O9 10 7' ,- "2..1' : . '0: .J:' * . : .J Control information: Acceptance criteria Summary of test endpoints:
% Mortality: I /. -20% 7-day LCso 1C07,,
-Average weight per initial larvae: g."-v15 ..i..: :. .:. :, NOEC 100 Average weight per surviving larvae: 0. -11i 0.25 mglarvae LOEC I0072.
ChV
>l07 IC25
Page 2 of 6 Environmental Testing Solutions, Inc. Date: Ik- I -C( Species: Pirmephazlespromnelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growsth Data Day CONTROL 11.3% 22.6% A B IC D E F G H I J L 0 00 1U /0 /0 /t /0 1(D /0 /(D /0 /0 10 1 '0O / 0 ,/0 '0 /0 "0 /0 0 ,0 (0 /0 2 /0 /0 (0 (0 /0 (0 /0 /o /0 to /.0 /0 3 /30 0 I'0 0 0 /0 /0 /o /0 '0 '0. /0 4
/(0 © /0 ( /0 /o /0 /o /0 /D /0 /0 SI /0 t 00 /0 10 i, 1 10 10 /0 to 6 /0D0 /0 /0 /0o0 /0 /t /0 /0 '0 /0
/0/0 1//0 , /0 /0 /0 q i /0 /0 A Pan weight (mg)
Color identification: r64.3R -Zv-i )'3.91 13.3b 15.3H 15.0`7 13.Oa 1H.90O 13.aa 13.(Db 6s.3 1,4..3S }5.ao 13.r"/r Analyst: LA E-B = Pan t" Larvae weight Analyst 3a-00 ao.'73 a3.-ý aa."731Qi lý Da.c.0 -,O 3a.q3 3 1 3a -.6 ,3.7Tq )3.-,kq a 1.)ýo Larvae weight (mg) = A - B 1.l~ " 1 .o.I- -1, gRo -1.,\ -I1-Lko J'. - -4 L-CA W eight per initial number ON of larvae (mg) rb v\O O*
'\9~~ =C / Initial number of larvae 0 'N A A*
4" 0' 0* 0* o* G Average Percent weight per reduction initial from control j 0 .1T .7, o cZ - number of (01 larvae (mg) Corunent codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.C Calculations and data reviewed-c: ..-- A
- LE ; .,-,m ntal Testing Solutions, Inc.
--. Xrli Page 3 of 6 I.' l l~ .. l Species: Piniephalespromnelas Client: TVA/ Sequoyah Nuclear Plant - UV-treated Date: t\- -
Surviva[ and Growth Data Day 45.2% 72.6% 100%_ M IN 0 p Q R S T U V Nw X 0/D' / /0 0 /0 /0 /0 /0 /0 /0 /0 /G /0 o0 (0 / 0 /0 1C /O /0 / 0 /D /0 2 b( 10 / Q0 /0 /0 /D //0 /0 /0 3U/. /0 tO /0 /0 /C /0C) IC 1c /0 /0
/O /O /O tO /0 /0 / O / /0 /'3 /0 /0 to /0 D0 10 10 tC I O 0 tO O t,0. CO 6 D0 /O 10 (C/ /,3 / /0 /0 /0
'7 k'
/ 10 /0 /0 /0 10 /0 10 / /0
/0 7
A= Pan "eight (mg) Color 4 identification: p-,-<r,, HSY515 .9 q ls .1q IA a il I",- r7 13 .(oq 1.3NIH..3 9 Nq 13 -9a
-99 5 0 9,a Analyst: _ _"_
B = Pan + Larvae weight (mg) Analyst: U a0.tO 19,) 9 a~ 3.44 3t-'40 aI.397 aa071,3 aa(c a~ll' ao'7Y Larvae weight (mg)= A - B lJ,* 4 -. -7... £.V,'(2.0 .l 1.-l -'.2. 5A m.oS r,3" i. 0 Weight per initial number..~A of larvae (mg) 0V \D' A Al/
= C/Initial number of larvae o ' " ' . .
0 0 , Average Percent. weight per reduction initial from control . 3 0. - 7,, .13\ 7. number of (/) larvae (mg) Comment codes: c =clear, d dead, fg =fungus, k =killed, m = missing, sk sick, sm =unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed:
lniviroflrfleftal I estiflg '-oluticins. In-c. i-age a or 6 Species: Pinmephalespromrelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: lk- il+Ot. Survival and Growvth Data Day 100% Intake Y [Z AA BB 0 Co 0 10 10 IC 0 / C) 20 D .10 0O 20 /) 0 ' 3 C 4
*_________ 6" /Q /0 /03 10 /0 /0D 0 6 6 (0 10 /0 /0
/0 (0 1t0 (0 A = Pan "*eight (mg)
Color identification: Analyst: U iS B = Pan Larvae weight Anail'st: R . Larvae weight (mg) = A - B Weight per initial number of larvae (mg) ,.. 4 "
= C / Initial number of larvae
" ' 0 0' Average Percent weight per reduction initial from control 0- 1 "Zl. 7 number of (/)
larvae (,mg) Comment codes: c : clear, d = dead; fg = fungus, k killed, m missing, sk sick, sm unusually small, Ig unusually large, d&r =,decanted and returned, w wounded. Calculations and data reviewed: Comments:
UV-treated November 1.4-21, 2006 Chronic Whole Effluent Toxicity Tcst (EPA_821-R-02-013, Method 1000.0) Species: Pimephalesproicelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2878 Reveirncd try: r l a'4 Nor Co Cor( rance AS2.ustnmot, Inec-na Labhorary QC _ _ _ _ ll~ t("') 0.lhl (lrec) A -B niib,, ollor'.,, 2ro!2 Stru-o.? rabl u 2' t*,"0 o2,oo, , oI- Irol (*1 InHi-l numer6 oS.r,0liof, ii... i.ir f 2 A 10 10 13 91 22.00 8.09 0.809 0,809 Control B 10 10 13.38 20 73 7.3 5 0 7 0775 4 0 735 100.0 0 771 4.1 Not applih C 10 10 1 .34 2323 7,89 0.789 0,789 D 20 o0 15.07 22.73 7.66 0,766 0766 E* 10 9 13 02 21A8 816 0 907 0.816
- 11. /,7 F 10 10 14 70 22.50 7.80 0.780 0809 9.7 0780 97.S 0 787 6.1 -28 120 20 12.22 2043 221 0721 0721 H 10 20 13 66 21 95 829 0,829 0 829 1 20 9 2. 36 72 64 7.36 0.807 0726 J 10 10 14.35 23.74 9.39 0,939 0 822 10.2 0.939 97.9 0.802 12. -3.5 K 20 20 I 5.20 23.24 8.04 0.804 0804 L 10 10 13.77 21216 7.)9 0,739 0 739 M 20 20 .1458 20 69 6.223 0.610 0,610 4952. N 20 20 15.44 23 58 8.14 0814 0 0.743 140 0814 0 100.0 0.74.3 14.0 4.
O 20 ;0 15.14 2226 7.12 0 712 0 712 P to 20 14 29 22 65 8.36 0.836 0 836 Q 2(0 9 15,57 2344 7.87 0 874 0387 72.6*.. R 10 "
- 10 13.64 21.40 7 76 02776 0.792 7.2 0.776 97.5 0.770. 2.0 0.6 S 80 10: 14.35 21297 7.62 0.762 0.762 T 20 10 12453 22 07 7.54 0.754 0.754 U 20 10 14 97 22 02 7.05 0.708 0 705 i00%/. 120 10 14.74 22 21 7.37 8 7.17 0.731 42 0.737 100.0 0.731 4.2 86 W 10 20 13.72 20 82 7310 0.710 0 320 X 80 20 15.02 22.74 .772 0 772 0 772 80 . 28 124.40 20.86 6446 0.646 0646 IttOy/ Intake 20 28 14 60 22 28 7 68 0.68 0.726 8.1 0 760 100.0 0.726 8.1 6.1 20 20 . 2464 1AA 21.81 7 17 0717 0.717 20 19 10 14 34 22 06 7 72 0 772 0.772 1 1 2Out8al8 882: MSD = Minimum Significan Dfrfetcnce Duorrett's MS3 value: 0.1095 PMSD Percent Minimum Significant Di-letence P5250 24.8 PMSD is a mseasore oftest precision. Ihe PMSD is the nullinrunt percent dil(crcncc betwuen the conrtol and reattmnt tnlatcanobe declated stalislically sipificar in a whole ement to0tcity Ic ave, age, a stgrt ficant diTctrence occurs (ot PEviionntental Testing Solutions. Inc chonic toxicity tests when a losicaul tedruce*s Pimephles gpowth by I S.0% flom the contol (detcirineii Nhto Intake: e f2r nce tonicant resting).
IDonne8t MSD value: 00649 Lower PMSD bound delemrnined by US-PA (210d percentile) = 9.4% PMSD: 8.4 Upper PMSD bound determined by USEPA (9006 percentile) = 35% Ihe lower and upper bounds were calculated by the USEPA using 205 tests conducted fror 19 laboratories for Prrepholes growth in chronic rftetnce toxicant Itsts US7PA. 2000 Underslandrng and A ccuontg 0ot Mluthnd Variabilily in Whole Efrln Ton oiciy Apphrcalions Under the National Pol itla,t t),schar ge Firritn.,i i , , ugrat. 0'A .830.800.003 U2S En'itoilninlaIl clron Agency noan, 022. 0*-C
UV-treated November 14-21, 2006 Statistical Analyses Lar',I Fish Groilh .nd SarvialI Twst-7 D-1 Croa.lh Sianl Dam I L,'14/2006 rcst ID PpFRCR S0,&pla ID, r,'A r Seqooylh NuIc>IMPL.14 0u>%* ;01 End Dala I 1/21/2006 Lab ID 2TS En'L, Tn*m, Sol SamopleType D'.I Dztsc~hag Mo-mto.~ng Rapc.'l Sample Dei Pf3c~P, A FWCHR EPA-821 .R-02 010 Ta: S:cces PP-?Pnphales prom!!ýs Comm -Inls V,.lrealed C - 3/. 1 2 9 D.Contol 0D090 0 7350 0 7890 0 ?660 11 3 0W160 07800 07210 0E290 226 0 7260 0 9390 0 8040 0 7390 45 2 06100 0 140 0 7120 0 3360 72 6 07070 0 7760 0 7620 0 7590 100 "07050 07370 07100' 07720 Traa a,3oa.- Untr- a farneed I-Tilad Ja0440 Con,%04 M-an I~a CYOS N.n N -Stal ('eSr uin Critic.l 7ISD MI1AA 7N,>-Mn D-C.nrrol 0 7746 1 0000 0 7748 .67350 03090 04 a 0 780 j 0000 11 3 0.7365 10153 0 7965 C 7210 0 8290 6 !46 4 .0 259 2,410 0 1095 0 7878 1 0000 226 0 8020 103S2 0 8020 0 7260 0 9390 12 157 4 -0 500 2 41 0 01 095 0 7878 1 0030 952 07430 09590 07430 06100 03360 13974 4 - 0.699 2.410 01095 07564 09602 72 6 0 7698 0 9935 07698 0 740 07M77 I 905 4 0 110 2 410 0.1095 0 7564 09602 O 0 7310 0 6435 0 7310 0 7050 0 7720 4 205 4 0.963 2 410 0 1095 0.7310 09260 ASt ifii, Cri OaI Skw Kir Shapuo-W dkls Ttl0,cva , nonnal d soNibuholw-. 001) 0.979922116 0684 0 130965238 11296243a7 6.a.Oi]rs Tasl tqd-ca!cn eq-a1 -aar44,s(p 0 035) 1226457691 15 08627224 f1lYpoathai T.at (1-tail, 0.05) NOEC LOS-C ChV TUI MSDU MrSp MSB MSE F-ProS. d3 Di.n'n s Ts! 100 >100 1 0109544232 0141393007 0002832567 00.4132 139 0.640509246 5, 18 Traatmnts vs D-Con*-ol Lin'-,r Setlrpoa~a6n 3I00 R-swnpl,,) Point % SD 9950 CL (F Ip) Skam IC105 91,252 ICI0 > 100 IC! 5 >100 10 IC20 >l 00 0 9 1C25 >100 IC40 > 1 0D 0f IC'0 >100 0 7 06 o 0 0.4 a 02 02 0l 0.0
-0..
0 20 40 60 . 10 700 120 DosD /. U oat-SR ponsa Prot 09 07 I-a1.11 0 05 ei-1 o1 qgnif-ca te 0 05 0 04 0.3 02 0.1 q,_ I I-I4-06dato
UV-treated November 14-21, 2006 Statistical Analyses l.arall Fi*h G rowth rnd SuroinaI Test-7 Day Grooth Sa: Date 1 1/1412006 Test ID PpFRCR Sanmple ,ID TVA/ Scqaoyah NMicda, Plat ltade End Date 1I121/2006 Lab ID E ,Enor, Tesring Sol Soinpl, Typc DMR-Dachoig, Monrito;i.,,g Repotn SOoisls Do,!, Pintosol FWCHR. 9PA-821-R.02-01) Test Species PP.Pimrphaks ptmc.-,as Coirinent- JUV rieakd Coc.-s/ 1 3 4 D Coniol 0 9090 0.7350 07890 0 7660 300 0 6460 07680 0 7170 07720 t Tr-nsform: Unlr-nsformed I .'[ailed Io ois Coe.,% Moon. N-MoN. Nleon Shin Moo 0'VV, N I-Slu CIiti,oo MSD Me.n ".NlMen D.Coiiol 0 77o8 1 0000 0 7738 0 7310 0 8050 4004 4 07746 I C000 JO 07253 09)68 0 7258 004650 07720 8 097 4 367 2 94) 0"0640 07256 0 9368 Auili. ry T ts Sut tiuCeeorl Skew Kurl Shapvo-Wii TesI ' mdle aat-* normal disoibution (p > 0.013) 0919678569 0749 -0,80552162 001712049 F.Test idcales eqa, oa'ancl> (p = 0.343) 3 316288853 47 46722794 lkypotheiso Teat (1-tail, 0.05) N1SD. MSDp NISB IMOE F-Prab d4 Homioscedaosic I Test md,ca-les no ssigsiicaxil dLfr-CD 6064918560 008793038 C, 0 0r3802 000223257 0.192823291 1, 6 tca*ncrnLs sD-Conrol Liner ]nt~orpoaloon (2206 o ompt(s) Point % SD 95% CL(En p) Skew IC05" 79 056 IC3O >100 ICI >100 1.0 IC20 >I00 .
>100 C275 I-240 >100 0 5 ICi0 >300D 0 7 indicates IC e-sltlnate less than the lo-est " iccin*action 06 006 0.4 0.3 02 0.I 00 6 20 40 60 80 100 120 Dose %
Do.e-tR. peose Plot 09 08 1-1.11 0 00 level 07 . ... .. ... ... .. .... .. .. .... .. ... ... .. .... . ... .... ... . of s5yrs0icarnce 0.6 05 S0a 03 02 031 0 o c zoc
.iqn _ I-1-06dato
UV-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-82 l-R-02-013, Method 1000.0) Species: Pimnephlalespromnelas DailyChemical Analyses Project number: 2878 Reviewed by: / Concentration Parameter Day 0 D"a y I Dav 2 D a), 3 D)ay 4 Day 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Fin pH (SU) 768 7.59 7.69 .7.48 7.57 7.20 7.49 7 29 7.50 .7 27 7,61 746 7.83 DO (ng/L) 8A 7.8 8.2 7 7 TS 7.3 7,8 76 7.9 7.1 79 7.5 78 Control Conductivity (pmhos/cm) 307 307 I 296 OF8 301 297 300 Teimperatutre (C) 24 7 24.5 24.6 24.4 24 8 24 6 24 8 24 6 24.8 243 24 9 24.5 24 7 pHt (SU) 7T68 7.53 7.68 7.45 7.57 7.17 7 48 7.29 750 7.27 7 63 7,47 7 83 11.3 0// DO (in/L) _ __,_8.2 .1 7.7 7_ 7. T78
.. 7.T7_8] 70 79 7 5 _7 S Conductivity (pinhos/cm) 301302 304 289 299 298 295
_ Temperature (C) 247 24.2 24.7 24.2 24 8 248 24 9 24.7 24.9 24 6 24 9 24.1 24.9 pH ýSU) . 768 7.55 7 68 748 7.58 7.18 7.47 726 7.50 726 7 64 7.43 7.82 DO (mg/l) 8,1 77 82 7 7 8.0 7.2 7.9 .76 7.8 68 7 8 7.4 79 Conductivity (Itmhos/cm) 281 285 2276 285 282 279 Temperature (°C) 24.8 24.7 247 24 2 24 8 24:7: 2424 79 24 9 24.7 249 24.2 24 9 nPl (SU) 7.69 7 55 7.68 7 46 7.58 713 7,44 7,2 . 749 7.26 7.64 7.43 7 791 45.2% DO(rnmg/L) 8.1 7.8 82 77 8.1 69 7.9 76 79 68 79 73 80 Conductivity (mlimos/ctn) 258 259 65254 256 Temperature (°C) 24.8 24.3 24 9 24.3 24 S 24.5 24.8 24.7 249 24 5 2,.9 24 2 24.9 p H4(SU_ 7,69 7.55 767 7740 7._5_1 7.1 1_7.43 7_25 7.48 T""59 7( 7.77 1)0 DO(ing/L) 82 7.9 811 75 8.1 6 8 79 T6 8. 0 8 1 8.0 72.6% Conductivity (Plmos/cn) 218 222 3U 216 225 20 219 Temperature (C) 24.9 24.3 249 24. 25 0 24.5 24 9 24.5 25 0 24.5 25 01 21 9 p1H (SU) 7.69 7.57_" 7.67 7.4_ "7 ___ _39 _7.57 7_ 36 7_45 *7.26_ 7._58_7.4 7 74 D1 (rMg/L) 8.1 79 8.1 7.4 7.9 6.8 8.0 7.5 8.0 6.8 8 74 8s0 100% Conductivity (}tmnhos/cmn) 188 192 202 1881 189 18_ . 190 Temperatume (C) .251 24.31 24.9 24.2 25.0 24.6 241.) pll (SU) 24 7 25 I 246 250 24 5 25.0 7.68 7 58 7.68 7.43 7.55 7.16 7 36 7.48 7.29 725 0 .7 57 77 7 72
%OIntake I10 DO(mg/L) 8.2 7.91 8.2 7.4 8.1 69 8.0 7.4 8.1 69 82 7 80
-Conductivity (..nhos/cm) 186 185 195 _ 190 192 184 189_
1 Temperature (°C) 25.0 24.4 24.8 24.2 24.9 24.4 24.9 24.8 25.0 24.6 25.0 24.3 24 8
Environmental Testing Solutions, Inc. 4_~j JitC UIl Species: Pimeph a/es promelas Date: - -O _ Client: TVA / Sequoyah Nuclear Plant -UV-treated flIL')/ ("hpmniern." 0 12 Analyst Concentration Parameter. CONTROL pH (S U) DO (mg/L)
".-V(9 q.5q
.8*
q q..2 ,
',".09.e *46- 2-20
-.3 Conductivity (p.rnhos/crn) A t9 _____
Tem perature ('C) "A -I _.__. ._ _ -'2_ _, . Z_ .- _. pH (s.u.) " -*(__ LO5 6 ý.(O@ "6yV . .r.'4 ,.fn - 11.39/6 DO (mg/L) 8-1 8..2._q . _- -2 Conductivity 3c O Temperature ('C) "tq. 1 . - " -1 . ., pH (S. U.) ý, 6 9j,5s'. 22.6% DO (m 0L)-0 Conductivity __________ Temperature ('C) -. L._,,_ 11--11. 1"4. 7 " . -L'4.1 45.2% DO (m~gL) .1 6Z. 0. - Conduc.ti vity
.(pmhos/cm)__
Temperature ('C) 1-4 * "__._, 2'.3 Zq. 3 2L ,i "_-___- pH (S.U) . __._q _.__- j4-f K4N0 - 9.L 72.6% DO (mg/L) .. _ __.__5 (Prnhos/cm), Conductivity,01 aP W N6* Temperature (QC) "q 2A a.* -q.4 "+ _ 7._S. 0-PH (S.U.) -tT ~ L.,4~q 3 2.5-1 q-13 100% DO (m/gL) 6.1 Conductivity (urnhos/cm) 1_9_1q____2_ _ Temperature (C) L<; 114.3 j -Z .. 2 .2 S .0 _2__._. pH (S.U.) :q.u _ ,.4_ _ .__ , , _ -____ 100% Intake DO (mgfL) q."L.Z : .8. Conductio~ty (pmhos/cm) ______ lef - Temperature ('C) 1S. 0 2_. _Z2 __ _. _.. -__. . Final In itialI Final Initialv Ij Final
ETnvironmnental Testing Solutions, Inc. ý;,- ý - I Species: Pimephalespromelns Date: I\- I -A- o(.c Client: TVA / Sequoyah Nuclear Plant - UV-treated Day 4 5 6 AnalYst V _ Concen- Parameter tration CONTROL ( Uq pH(Su.) . q D0 (magL) q-6 I i:. Conductivity (pmhos/cm)Ni Temperature ('C) z 'A -. .'L . -L, -.C1 -LA-, S - ". .7 pH(S.Ui) q6~E 3.29 ') (-03'N.6 5q 91 11.3% DO (mg/L) /).e Condu ctivity mprhos/cmn) A Temperature ('C) U.. , 2"- 1,z4. 9 IL -- I 2O26% DO (mgL) ,, Conductivity p H (S .U) "44-!5 ".2'3.,/ (. L'4 2-2 g.-/ -02 q ( rn-hos/crn) Temperatre (°C) .t" 4.-1 A. IA Z-4
-- 2q. L, PH (S U) 1q.4 L4q . o. 1 I q,.50 I/
4726% DO (.m )-. 0 Conductivity (mhos/cm) I 1 I Temrn erature (°C) .. *1 .. . 7A Z4.O , "L 2 .. pH (SU.) 7-(o, 2 -.21 71,6% DO (m g/L) E3-0 Conductivity (Prnhos/crn)1qD-z0 (SiU) ItH 2 (a l 100%/ DO (rnIL) Condluctivity0 (prmhos/crn) 60 Temperature ('C) Ternperature (°C) 2'4! 'T1*'* 1. 2q-. <.O -c 2 ?Aqt
. 25.O q3-S.O 224. _. - q /
100%/ DO (mgIL) . Conductivity q7 1ec (Hrnlos/Crn)I Ternpcrature ('C) 7q.9 2L 40
.Lq 2 (-C0 2-5. 0 ~ zLi Initial ia Inta Final In ia Final Initial- Final
tLnvironmentat 1 esting t~olutions. iiiL. Alkalinity (EPA MNethod 310.1)d Matrixv Water, MDL= .0 mg CaCO,/L J, rrq 7T Analyst Tm ej4o 183~ Date analvzed ) .-) , Titrate samples to pH = 4.50 S.U. Titrant norrialiryand nultiplierdetermination: pH of Normality Normality (A') of HtSO, pH Factor or Multiplier Deionized Titrant cbeck Begin End Total (S ml Na2COt x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml 0.2,S/E Nx 500 4.5 S.U. number number (E) (acceptable range= 0.18- 0.022) 653 1AP] C 011 . 0. OO} 10.3 L Aoro ator control stadrdar." Reference True value Sample Alkalinity (M.V) %/o RS MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (rmg CaCO1 /L) (acceptable range (r.(g CaCO,/L) (ml) ml ml ml =90 to I /10%)
/J Z 100 100 19 P ?b Q4 Ij Duplicate sample precision:
Sample Alkalinity %R-PD Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L) ((S- D) /l(S+D)f2() x100 number (ml) ml ml ml (acceptable range 10%) 1+/-
.5-0'O _ _._O_1-_6t, 0 1o9.v 32 1(03 s 33
- Duplicate . 15 .O 3.2 D 33 Maftrix spike recovery:
Reference Spike-value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (rag CaCOj'L) (mg Ca.CO)/L) (ml) ml ml n il
-O . 32 *J 3.
Sample alkalinity (B) Measured spike.value (MV) % R =MV / SV x 100 (mg CaCOi/L) WV = A - B (acceptable range (mg CaCO3 /L) 75 to 125%) 33 Ibo!. Sample measurements: Sample volume Begin End Total Alkalinity .Sample number Sample ID (ml) . ml ml ml Multiplier (mg CaCO,(L) i.I n A M 20 " 30 3. ( 6-@ . q o( l.-L.cC - _ _8_ . 1.5 5to o _.__.______ 2+/-C iL _ i'.5 v-vD ~5.-5 - I -CS-oeo I.2.- ao.2 .33 0o0 111-.o1 - ' - - ., ,A.2- +.O '4 Oio Ill'.O1 A.?, Aa_ q / __ 3, 7T6___ 13 25 3 S Reviewed by. Date reviewed 1 1i Z7
Environmental I esting Solutions, Inc. I -ýE -_- - I Alkalinitv (EPA Method 310.1) Matrix: Water, MDL = 1 0 mg CaCO 3 /L Analyst Dae analyzed 0 =o Titrate samples to pH - 4.50 S.U. TiYranrt norma[ir, and muiptlher determinaton Laboratorycontrol standard: Reference True value Sample Alkalinity (IV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total NMIultiplier (mg CaCO)IL) (acceptable range (mg CaCO,,'L) (ml) M ml ml = 90 to 110%) Duplicatesample precision. . Sample Alkalinity %R/PD Sample Sample ID volume Begin End Total Multiplier (rag CaCO)/L) ((S - D) f/(S4D)/2l II 100 number 13 (ml) ml ml ml (acceptable range = 10%) o~,t4\,oI7- FD* Duplicate _.W Z 0oD 0 3.5- 3.5 Io. s_,3_ D Matri sike recov ery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mg CaCOi/L) (ml,) ml ml ml E50 ., 3.5 19 o.q 10.3 Sample alkalinity (B) Nleasured spike value (MV) % R MV / SV x 100 (mag CaCO3 /L) MV = A - B (acceptable range (mg CaCO3/L) = t5 125%) to 6D Ioo Sarnile measurene,,ts: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO,/L) o,,_\v,.*- 3 l0,3 l1.f lT@35
- i bA.3
.q d 0.39 t3 1 (0 o iw-.o A\ 1 IiA,*, 9(5,0 U.,4, O~ll ll.l_ B*3q-3 qbO P5 o'q,2,C.K" __"13 Re,,iewed.by40.-S I t (',.o'/ Date-re.iewed ____- l'@
Reviewed by I rý Date reviewed: F 1k-71K :o=(,,
F-11 "I I U11111t:11 I'll I C-') L1111L OVI U tt'j-. -- Alkalinity (EPA Method 310.1) Matrix: Water, MDL 1 0 rg CaCO;'L Analy)s t fAL"" Date analyzed t Titrate samples to pH = 4.50 SU. Ti~ranr nortncalirv and n ulriplierdetermination. Normality Normality (.M of H2 0o, pH Factor or ,itutiplier Deionized water fI ra, reference AP standard - _..egin nil End ml Total ml (S ml NaCO)/E"N--50x 0.05)/E - (-Nx 50000)/ 100 nl sample' 5/E .= ,Vx 500 _= 4.5 S.Uý num ber num ber F (E) (a ..epabIe ran oe 0 Laboratorycono-ol standard: Reference True value Sample Alkalinity (MIV) RS MV /TVx 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (rag CaCOv!L) (ml) ml ml ml 90 tot 10%)
.. ,(L 22.3 100 100 .3 3-0 c.-4 Jo.3 Io0), tb6, 61.
Duplicatesample precision:. Sample Alkalinity RPD
.Sample Sample ID volume Begin End Total Multiplier (mg C3CO3/L) ((S - D)!/(S÷D)/2l1 x 100 number (ml) ml ml ml (acceptable range = 10%)
11*,4'o5 /._0d~a,.l_ AS 511~ -'.S 1.0 ý')1.t3 s JaO. Duplicaftc 3 e, 3.o D o __________ Matrix sspike recovery. " Reference . Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mag CaCOYL) (mg CaCO 3/L) (ml) ml ml ml
ý3~.Z 9 7g-9 14 10,3 Sample alkalinity (B) Measured spike value (MV) % R =MV / SV x 100 (mg CaCO3,'L) MV = A - B (acceptable range (Mg CaCO3 /L) 75 to 125%)
' o I0
/O0,0/'
Sample m easuret ents." Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml !Multiplier (Mg CaCO3/L) o .2 ., L0. 19 ?l';) 1.3 1a 0 or,~
~ ,, ~ gq6q, ~ ~ ~~3 c)g\_3.)D .}
v,*Xq j*,j/ 3Ob 3 .o 3.o 3-5-352__ 2
,,,_ t0.O q.o 5.o
.W1.
2
/
3~ ~ lo~ U027 O iQ l .0.l, 3S 35q lfl.oZ3 . o._ 0.2.-, _./
-
- I- 5p~h0.O,!
15.O~s ,:.o. ,a.. ,*
- Reviewed by: \III Date.reviewed: [ Ih- tqO-bt
Alkalinity (EPA Method 310.1) Matrix Watcr, MDL-- 1.0mg C3CO3JL Analyst )' ,.., Date analyzed I 'I @7.)k9 Titrate samples to p1H = 4.50 S.U. Trrr~at~zg.jj, ma/icy and mijlrinlipr di'rprrninatiori: PNormality (.N) of Fl,SO* pI- Factor or iMultiplier Deionized Titrant check Begin -Ed --Ta(S ml NaC0 3 x 0.05)'E ('x=x(.",' 50000)5000)100 1 0mml ssample pe water reference stan~dard ml ml MI 2 5,.-V x 500
=4. 5 S. U. number o numbecr (E) (acpt abe rang =0 ."'_
Laboratory control standard: Reference True value Sample Alkalinit), (M'-) % RS NIV T\V x 100 standard number .(TV) volume Begin End Total Multiplier (rag CaCO,'L) (acceptable range (mg CaCOIL) (ml) ml ml ml 90 to 110%)
,100 loo -3 D, D.o/-
Duplicate sample precision: Sample Alkalinity . %RPD = Sample Sample ID volume Begin End Total MMultiplier (mg CaCO,!L) (S - D) /I(S-D)/21) x 100 number (ml) ml ml ml (acceptable range = 10%) 541. ,so Duplicate 313 0 ) 0cq
- l
- 4. to 0
o._ s_ D 1 ..00['O Matrix spike recovery:,. Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total MNiultiplier (mg CaCO,/L) (rag CaCO,/L) (ml) ml ml ml 6D 10 DO(. 03 (S~- Sample alkalinity (B) Measur~ed spike value (M1V) % R MVN// SVx ]00 (mg CaCO,/L) IV = A - B (acceptable range (mg CaCOiL) 75 to 125%) Sample measurements:. Sample volume Begin End Total Alkalinity Sample number Sample ID ml) ml ml ml Multiplier (mg CaCOL)
*.0, S .jf V 10.3 q-4 C)~
11.,. . _ Reviewed by w3 Date reviewed 1 - 1Fo
f I1*11 Ulllll*J* .... Total Ilardness (EPA Method 130.2) Matrix Water, MDL 1 .0 mg CaCO 3 /L Arialyst A.Time mit at-d. Date an~alyzed -iTime completed: Tirrant normalitv and mu/tiplier determination: Titrant' Normality check Begin End Total Normality (N) of EDTA pH Factor or Muliplier reference standard mi ml ml = 0.2[PE= (N/x 50000)1/50 ml sample number number (E) (acceptable range = 0.018 - 0.022) (Vx 1000 gi) I 4 Dý 6.0 R.2 ,, Oo Q. Laboratorv control standard: Reference standard True value . Sample Hardness (I1V) % RS NIV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOYL) (acceptable range (mg CaCOjL) (ml,) ml ml ml 90 tot110 oo) I 4,,l4 40 50 Il.e 1OO l*q 210.z2 6 c 0 .O. Duplicate sample precision: Sample _ _Hardness %-RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCOs/L) ((S - D) /(S*-D)/21) x 100 number (m__) ml - ml ml (acceptable range=+/- 10%) 1).1,5 .0. . . . . .. .. 1..0 q..o ... .. . 2 . . S.. . ...... .... .......... ..... .-.. " t~~l~54~ 'SD S~~c,~-)I~~i~D lj.C iLLO ~~~ ~QO.2
.... .. :...i...c.2.,7>Z .......
Dup*catei(B) H 0 kaOI, " D .{2- L4,_ - Matrix spike recoverws. Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mg CaCOsfL) (ml) ml ml ml
,* '-tO bL iq.0 _ ,o. z 3+* -.
Sample hardness (B) Measured spike value (V) aR= pble SV x 100 (mag CaCOJfL) M'V = A - Bl (acceptable range (mg CaCOjfL) = 75 t6 125%) L12 4 02 Sample measurements.- Sample volume Begin End Total Hardness Sample number Sample ID (ml ml ml ml Multiplier (rng CaCOsfL) Blank
- t-,O (should be = 0 mg CaCOi/L) 0.0 0.0) /)f.
UO. ("4 I .-IrsLo **q _12_ __._____
. -o., . Q.'-- O3 11.o-Ou 5SoU. Hz 0 _.' q3.7 ,,-21 J 2Z oo,,,,tQcak J-j _ck_1 0"0 L4..9q
,*.o, fl*O1 _ aq.g. 4.j *Y __ __ _ __ _- _ Note If >1 5ml of titrant is used, sample must be diluted. Revieýved by Date reviewed 19. Oil.
Environmenital I esting 5)Oluttons. Inc.________ Total Hardness (EPA ,'Iethod 130.2) N'atrix. Water, MDL = 10 mg CaCO5/L Analyst 1 Tirme initiated: Date analyzed J I6y Time completed: Titrat normality nad multiplier determination: pH Factor or Multiplier (Ax 50000)/ 50 ml sample
='.* 1000 Duplicaresample precision.:
Sample IRPD Hardness Sample Sample ID volume Begin End Total Multiplier (mg CaCO3.fL) ((S - D) /[(S*D)/21) x 100 number (m ) ml ml I m0%) (acceptable range
,btq. t , 0u" - 1J) . .5 _ _ _ _ i . II 22 SL ... . .
Duphcaite(B) - i" . .l ,. Matriv spike recoaverv." Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mg CaCO3L) (ml) ml mIl ml
, sLo - i iI-.
JSample hardness (B) Measured spike value (MV) % MV / SV x 100 M (amg CaCO)/*L) NlV =A - B (acceptable range (mg CaCOfL) 75 to 12S%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml ml ml ml Multiplier (mg CaCO)/L) Blank
"_ _' _ (should be - 0 mg CaCO 3/L)
TqA"S.W*fol _1 "(13.2 9 .1.1 14-1 il 13 11,.* FD'z 2-,12 Le o. 6
**gq "q
~i3 ,k 1u M.
o,1 5-30~ T-31 o B 14* 3.6 -71q a,.,,,-.,q1 . l*3.* 0*.03.63-"7 o*,n.,o* 3 , 3&.-------.-----/ Note If >1 Sml of titrant is used, sample must be diluted: Reviewed by: j Date reviexed r o- 1i)-O(_ i
Page PP~e*f _ Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL-0.10 rnm/L Meter: Accurnet %ModelAR25 pHIon Meter Arn alyst Iodide reagent: I Date analyzed Acid reagent: r Calibration: Reference standard number t0, " 5-,31-3 Note. For samples with a resrdual chlorine of > 1.0 rag/L, the calibtation range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (,',IV) IRS l=1V / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 0 - 4i Ci e ,(,:0/ Duplicate sample precision: Sample " ID _Sample Sample characteristics Residual chlorine %/RPD = ((S -D) /[(S+D)/2j) x 100 number __mg/L) (acceptable range +/- 10%) fNJO (nci'lr Duplicate D1~XYO3 Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number _(img/L) _ _ _ _ _ _ _ Blank (should be < 0.10 mg/L) ,X K.I,'
. .. . .:. " :. . 4© 99'>
g&,,x~oe ate - 5'- 9k-+W doud,4 -/ <Ho. oo sl t)(oijjbtC'Fe- §'c- YPI-En VUhh oO'(cIqu a1UC i t~i~(oO-
,oY*/I *q~c,5 e l7-ia,-< .
i.-- c keo ,,r,~ coa o or ( 0.oo oo'4q% _______5 .t _ ,4 IF- Ctca. 0 Q, ,NoCi) r _OO.___ Note: All samples were analyzed in excess of EPA recommended holding time (15 minmutes) unless otherwtse noted. Laboratory control standard: Reference standard True value (TV) . Measured value (MV) % RS */1V TV x 100 nunsber (mgf/L) (mg/L) (acceptable range = 90 to 110%) IF, 0.50 0 Q. (Oi.
*,? ,° Reviewed b y.,
*Date reviewed LL Lie Ž
Page 1 of Total Residual Chlorine (EPA. Method 330.5) Mat"rix Water, MDL = 0 10 rn/L M-,,eter. Accumet Model AR25 pW,'lon Meter Analyst OýA Iodide reagent: TN2 )"3c A Date analyzed Acid reagent: T--Nz q Calibration:
.se,,'netdr andard:'Reference n r I 0,10 mg/L 1.00 mg/L I numnber INS' Note For samples with a residual chlorine of > 1 0 mg/L, the calibrarion range must be adjusted to bracker the chlorine levels of the sa-mples.-
Laboratoro, control standard: Reference standard True value (TV) Measured value (NIV) RS= MV /TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) m c0.50 0 =-I ýac Duplicate sample precision: _ 0 Sample Sample ID Sample characteristics Residual chlorine / 0RPD ((S - D) /I(S+D)/2j} x 100 number ' (mg/L) (acceptable range 10%) O',m\R*~ o/r ~~C). c.ccor-eae_ Duplicate ......... IS :" D"0 0000133 I. O<. r:'yt.C " }.' --, .7i':;b .. *i ..*..V* Sarnple measurements: Sample Sample ID Sample characteristics Residual chlorine number - (mg/L)
.Bl.ank (should be " 1.
0..:<,0.10.mg.).. .. ,1* . . . . /.- 0 3 'O AQSooa ýA Wo0YIcicd(N MI ofi~ olni.. o\ CWYt& CI, CXVe* t cc. 0 nY 1-Cioo0o08ooo dj~ylde 0\a c...... 00coli 0_ 1 L< TklIq- .uaN- MIt CA &* tx-' c-) ir NJo *o- Coo0orc3f liIJ 06,1 ., -\'1 -P ,- TTNT cýow , 1\)C cc\OrQa-
-- - I-------------------rn 1 '~cc~vL.O0'3 Note. AIl samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherAise noted.
Laboratorv control standard: Reference standard True value (TV) Measured value (MV) %RS MV /TV x 100 number (mgfL) (mg/L) (acceptable range = 90 to 1100/o) 0.50 0.53 1-7 f", 0 o Reviewed by Date reviewed
Page 3a_ Pace t S Total Residual Chlorine (EPA Method 330.5) Matrix Water, MDL = 0 10 mg/L Meter Accumet Model AR25 pHion Me:er Analys! Iodide reagent: -- Z35 Date analyzed - - Acid reagent: -T*fZ '.-o 7 Calibration:'
. .. .. . I .10mg/L 1.00 ml`L I Reference standard number .. .. " SS& 3 X NSrt 1 j Note For samples with a residual chlorine of'> 10 mgL; rthecalibration range must be adjusted to bracket he chlorine le ,els ofthe samples Laboratory control standard:
Reference standard True value (TV) Measured value (5NIV) % RS = MI"V / TV x 100 number' (mgfL) (rmg/L) (acceptable range= 90 1 /10%) m=NsS3S3 .. 0.50 ,. 18. 7 Duplicate sample precision:. Sample Sample ID Sample characteristics Residual chlorine %. RPD = (S - D) /I(S+D)/21) x 100 number _"__(rag/L) *_._.____ *(acceptable range = 10%)
- >" : .0.0000 a L),0 "
Duplicate D o.ooO5C Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number. (mg/L) _______________ Blank (should be < 0IC
.,' !!L) 0, mg., ,. - :.
0ý0 I C)Oriýp 0-00 8"D~e 3_N(~coS O WI.0 -- 1 k4
- y ,L) c leo.t qp ak y C0 o.0 1 9 t,\-fl\1 eo2v SBa a..v- c Te I- _._c :"O019r' o") Cc NA-3 wpt d-yoy, ýý ( J cO e OCto 1 -3.l5 ___ C C1Ot5- , cOICY OtJI1,.o* __, __...,,,_ c.ae.v, \Q co cY- Lo0 occIH1 iote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless other.wise noted Laboratory control standard:
Reference standard True value (TV) Measured value (MV) % RS NI'V / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to I 10%)
-,50 o - Hq 3 q i .01 Reviewed by Date reviewed
Pape "33 Pagee _ of -- Total Residual Chlorine (EPA Method 330.5)
,Matrix: %k'ater,MDL = 0, 10 mg/L.
Meter: Accumet NModel A.R23 pH/lon Meter Analyst Lfl Iodide reagent: N Date analyzed - o, Acid reagent: - Nr ..F7 Calibration:
'.. . "I 0.10 mg/L 1.00 mg/L" Reference standard number 3- T-,6S 353 Nore. For samples with a fesidual chlorine of > 1,0 m /L, (he calhbraiorn range must be adjusted to bracket the chlorine levcls of the samples Laboratory control standard.:
Reference standard True value (TV) Measured value (,IV) % RS =MNV/TV x. 100 number (mgJ,,) (mg/L) (acceptable range = 90 to 110%) Duplcate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD= (S - D) /[(S+D),'2]) x 100
-number a(mg/L) (acceptable range 10%)
.1 :'Duplicate D Sample meaSurements.:
Sample Sample ID Sample characteristics Residual chlorine number (mz/L) Blank (should be =< 0 10 mgiL) :- ' . . -- e. 2i.
.,, (1.. Ao v C C4-Yr V,teP OeCt, NO ._01O0YO O4 Il' .3 lilb .05 o(a i .flq "h Cs u N+,,*cj N o,, y " , .o)oQ ble~l .l Cvn a- 4C.Oa -ký oo0o.z aoZ 1o o*,ll8S.C~o "P,.0,e£*V Nu SC._ c/£e ..vw *o *o\Ov- L OC .OCOOI83 0W 1. 001 o0 Ct-fOC)C 0c4*o,. OOrcNC 18,3
__ _.*__ _ _. .V I .O000 is OCle 0I JJl6 22 I C_ e Ne c.)b.V : .,*_., 3 -9 Note. All samples were anal yz inescess of EPA recommended holding lime (15 minutes) unless the.rwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) /% RS- NMV TV x 100 number (mg/L) (mg/L) .(acceptable range 90 to II 0%/) -[TN s530.50 T Qr-q/, o Reviewed by Date reviewed - L- O
Page -311 Page 3 of 3 Total Residual Chlorine (EPA Method 330.5) MatriX: Water, MDL= 0.10. mg/L Meter Accumet Model AR25 pHi'on Meter a nanysd*I Iodide reagent "T-Date analyzed Acid reagent Z Calibration:
- >. * . I 0.10 1.00 mg/
Reference standard number N S S3c" 3 "jNS5-3S3 Note: For santples with a residual chlorine of > 1.0 mg/I.. the calibranon range must be adjusted to bracket the chlorine levels ofthe samples. Laborator}'control standard. Reference standard True value (TV) Measured value (MV) % RS =MV/TV x 100 number (mg/L) (mgfL) (acceptable range = 90 to 110%) Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD= {(S D) /[(S+D)/21] x 100 number (mg/L) (acceptable range=+/- 10%) Duplicate ,, - D L.O . _..,,_ _
~Z Sampl1e measurements.:
Sample Sample ID Sample characteristics Residual chlorine number (rng/L) 0 .. 'be B.a n ( h ud. < 0 10"...... .. . . .. . Note: All samples were analyzed in excesrfPA recommended holding time (IS minutes) unless otherwise noted. Laborator}, control standard: Reference standard True value (TV) Measured value (MV) %RS= MV TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 .q47l4 q. 0t ./0 Reviewed by. Date reviewed
Sequoyah Nuclear Plant Biomonitoring November 14-21, 2006 Appendix D Reference Toxicant Test and Control Chart
Sif .. ,a.... . V sa .. . . . -. Pimephales proinelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 USEPA 1T I I I I 1 I I I I II I .;
*USEPA Co~ntrol Limits (+ 2 StandardDev'iations~)
0.8 0.7 0.6 0.5 H I I I I I I I I I I I I - I I I I 1 '") .........
. I I I I I I I I I ( t a , i C s) I and Control Limits (75'j and 90t Percentile C's,)
USEPA Warning 1.0 0.8 0.6 0.4 I I I - - - - - - - - - - - - I- -- 0.9 0.8 0.7 0.6 0.5 0.4 Test date 7-day I C = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Piniephales growth for the test population. Central Tendency (mean IC 25 ) ....- Warning Limits (mean IC25 ":+/- S A10 or SA.75) Control Limits.(mean IC 25 +/- S A.25 S,\ 90, or 2 Standard Deviations)
Environmental Testing Solutions, I nc. Psi'ephralespro nclas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water State and USFPA Laboratory Laboratory Test tiunmher USEPA USEPA Test date 7-day IC, 5 CT S Control Limits S..o) 'Wirniig Llniits SIX Control Limits S.1,, ,.'arinig Linits S~,i Cotr'ol Limits C (giL KCI) (gfL KCI) CT-- 2S CT+ ZS CT-S.. CT S, CI - Sý, CT 4 Sý, CT -S,,, CTr+ S, cr - S*, CT +S, 1 12-06-05 061 2 02-07-06 067 0.64 0.04 056 0,72 0.08 057 072 013 0.51 0.78 0.24 040 089 0.29 035 093 01 3 03-07-06 0.61 0,63 0.03 0.57 070 0.08 056 0.71 0,13 050 '0.77 0.24 0.39 087 028 0.35 092 01 4 03-14-06 0.53 0.61 006 0.50 0.72 0.07 0.54 0.68 0 13 0.48 0.74 0.23 0.38 0.84 027 033 0.88 0, 5 04-04-06 0.57 0 60 0.05 0.50 0.70 0.07 0 53 0.67 0 13 (0.47 0.73 0.23 0.37 0 83 0.27 0 33 0 87 " 0 6 04-18-06 0.64 0.61 0.05 0.51 0.71 0.A7 0 53 0.68 0.13 0,48 073 0.23 0 38 0.84 0.27 073 0 88 0( 7 04-18-06 0.61 0.61 005 0.52 0.70 007 0.53 068 0.13 0.48 0.73 023 0.38 .084 027 0.33 0 PQ 0.1 8 05-09-06 0.57 0.60 0.04 051 0.69 0.07 0.53 0.67 0 13 048 0.73 0.23 0.37 0.83 0.27 0 33 0.87 0' 9 06-06-06 0.45 0.59 0.07 0.45 0.72 0.07 0.52 066 0 12 0.46 0.71 0.22 0,36 081 0.26 0.32
- 10. 06-13-06 0.85 0.
0.56 0.58 0.06 046 0.71 0.07 051 0.65 012 0.46 0.71 0.22 1136 0.8F 0.26 0.32 11 07-11-06 0.85 () 0 61 0.59 0.06 0.47 0.71 0.07 0.52 0 66 0.12 0.46 0.71 0.22 0 36 0.81 0.26 0 32 0 85 0 12 07-16-06 0.61 0.59 0.06 0.47 0.70 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 O.81 0.26 0.32 0.85 0 13 08-01-06 0.64 0.59 0.06 0.48 0.71 0.07 052 0.66 0.12 0.47 1272 0.22 0.37 0.82 0 27 0 33 086 0 14 08-08-06 0 67 0.60 0.06 0 48. 0.71 0.07 0.53 0 67 0.13 0.47 0 72 0.27 0 37 0.82 0.27 15 033 0.87 0. 08-15-06 0.49 0.59 0.06 0.47 0.72 0.07 0.52 0.66 0 12 0.47 . 0.71 022. 0 37 0.81 0.27 0.32 16 0.86 0 08-22-06 0.65 0.59 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47 0 72 0.23 0.37 0.82 0.27 0.33 17 09-19-06 0.86 0. 0.61 0.60 0.16 0.47 0.72 0.07 0.52 0 67 0.3 0.47 0.72 0.23 0 37 0.82 0.27 0 33 0.116 0 1I8 10-10-06 0.60 0.60 0.06 0.48 0.71 0.07 0.52 0.67 0.13 0.47 . 072 0.23 0.37 0.82 0.27 0.33 .0.86 0. 19 10-17-06 0.64 0.60 0.06 0.48 0.72 .0.07 0.57 0.67 0 13 0.47 0.72 0 23 0 37 0.83 0.27 20 0.33 0 87 0 11-14-06 0.54 0.60 0.06 0.48 0.71 0.07 0.52 0.67 0. 12 0.47 0.72 0 23 0 37 0.82 0.27 0.33 0.86 0 oVte' 7-d iC, = 7-day 25% iaiibitibn concentration. An csibnation.of the concentnaaion ofpolassium chiloridethial would cause a 25% reductionl in Pinsephales growtl for dlie test population. CT Central tendency (dsean IC,,). S.= Standard deviation of the IC,, values. , Laboratory Control and Wanrutg Limuts Laboratory control and warning mits were etabLished using Vie standard deviation of the IC, values corresponding to the 10thsAd 25th percentile CVs. These ranges arc nmore surugent thian lie control and ,,,ang liriwts recommended by USEPA for Vie lest method and endpoii S, = Standard deviation correspondoing to the 10"' percentile CV. (S. 012) 0n, S,25 = Standard deviation corresponding to the 25lfpcrcettle CV. (S.,2 = 0.21) USEPA Control and Wanuing Liersits S,15 = Slaiidard deviation currespunidatgto the 75 percetitle CV. ( S, = 0.38) S,90 = Standard deviation concsponding to the 90" percentile CV. (S,9 0.45) 0 CV= Coefficient of variation of the ICs values. USEPA 2000 Understanding and Accoutimng fot Method Vaiiabiity is Whole turent Toxicity Appphcati*ns httderti(e National Poultaitl D'Schaige luinmahtonPiogiam EI'A. 833tK00.003 US lHevuoinunenUtl Pintectisn Agency. Carionati, OH. O~gcun;nbsiedrreAquo&re Binlynsem. I-, 11 14-06 1140
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimephalesprornelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test date Control Control Plean Test number CT CV CT MSD PMSD CT Surviva) Growth (0)g) for Control Growth (mg/larvae) (%) . for Control (N) for PMSD (%) Growth CV (%) 1 12-06-05 100 0.626 7.1 0.06 10.0 2 02-07-06 100 0.711 0.669 7.8 7.5 0.09 13.2 11.6 3 03 06 100 0.774 0.704 8.4 7.8 0.10 13.3 12.2 4 03-14-06 100 0.745 0.714 9.7 8.3 0 14 18.9 13.8 5 04-04-06 100 0.766 0.724 11.5 8.9 0.08 10.7 13.2 6 04-18-06 100 0.621 0.707 9.7 9.0 0,09 13.8 133 7 04-18-06 100 0.668 0.702 13.3 9.7 010 154 13.6 8 05-09-06 100 0.841 0.719 7.5 9.4 0 12 14.4 137 9 06-06-06 100 0.783 0. 726 5.9 9.0 0 08 96 13.2 10 06-13-06 97,5 0.709 0.724 8.2 89 016 22.0 14.1 11 07-.1 1-06 100 0.673 0.720 2.5 8.3 009 13.1 14.0 12 07-18-06 975 0 623 0712 17.1 91 0.10 16.6 14.2 13 08-01-06 100 0.746 0.714 10.5 9.2 0.13 17.0 14.5 14 08-08-06 100 0613 0.707 10.4 9.3 0.08 13.6 14.4 15 08-15-06 100 0 765 0.711 8.0 9.2 016, 20.4 14.8 16 08-.22-06 100 0.768 0.714 8.7 9,2 0,12 15.5 14.8 17 .09- 19-06 100 0.699 0.713 12.7 9.4 0,12 17.2 15.0 18 10-10-06 100 0.685 0.712 5.1 9.1 0 10 14.0 14.9. 1.9 10-1 7-0.6 100 0..781 0.716 7.3 9.0 0.15 18.7 151 20 1.1-14-06 100 0,737 0.717 5.3 8.8 0.10 13.4 150 No te CV = Coefficient of variation for control growth. On .average, the CV for control growth is 8.8% in Environmental Testing Solutions, Inc Pimepholes chronic toxicity tests Lower CV bound determined by USEPA (100' percentile) 3.5%.
..Upper CV bound determined by USEPA (905 percentile) 20%
MISD = Minimum Significant Difference PF'SD = Percent IMtirtimun Significant Difference PMSD is a measure offest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant diflerence occurs for Environmental Testing Solutions, Inc. chsonic toxicity tests when a toxicant reduces Pimephales growth by 15.0% from the control Lower PMSD bound determined by USEPA (10' percentile) 9.4%. Upper PNISD bound determined by USEPA (90 h percentile) 35% CT Central Tendancy (mean Control Growth, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales grow,,th in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variabiliry in V/hole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. Organisms ohsoiored from Aqvotic BioSy-slens, Inc.I1-49 H14-,-0.6
PimephalespromelasControl Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25 I I I I I I . I I I I I
*'" 1.00 0.75 0.50 0.25 LISEPA Acceptance Criteria (> 0.25 mgpcr surviving larvae)
I-1 I L I 1 II I I I I I I I I I I II I .1 I I I I I I I I I* C 0 30 Kentucky Acceptance Limit (< 30.0%) 20 ,- ~- 0 10 0 0 0 i i, - i I I I I II I I I . I I I I I I 1 ] 40 USEPA Upper PMSD Bound ( 9 0 h percentile <. 35.0%) 30 20 10 n M/ I E II I I II I I I I I I ' I I Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2Standard Deviations)
Environmental Testing Solutions. Inc. Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Piniephalespromelas PpKCICR Test Number fi. Diiution preparationinformation: Comments." KCI CHM number: F __.__Z(_":I Stock preparation: 50 g KCI/L. Dissolve 50 g KCh nI -L Deionized water Dilution prep (rmg./L) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 i8 Diluent volume (mL) 994 99t 988 985 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information:. Organism age: -2Zis -I1 -z,4 :'1S A*J._ts C)L_ b Randomizing template: el),"L e_ Date and times organisms I .- c)(_-oq otlOO -o lcO Incubator number and 5F were born between: shelf location: Organism source: " .6 ý>A'vLA 11
, 11.-o0( Artemia lot'number ' 12.1Y4 0 Transfer bowl informnation: pH = SU Temperature 'C Total drymg time: ____z':-___
.'.2 -Date/Time in: ti-1\-OL,,J _So Average transfer volume: . Date / Time out ",.r-o p4_o_ u Oven temperature: .c C, Daily feeding and renewal infornation:
Day Date Morning Afternoon Test initiation, lIHS Analyst feeding feeding tirne renewal, or batch used time termination time 2 rtO C) I cO i'-C62Oo 2 3 01.ý-o',. /00.2 tb l . iO 1l-0-o(tA _ l-&9-ot , zA 8 i -s-o (3 6 It-2O -Ot . b l 0
)to_-2-._\_,
7: " _______ fV2IO~ + K..K.cr. Control information: Acceptance criteria Summary of test endpoin'ts:
% Mor'ality: 07 S 206% ,7-day LC50 Average weig t per initial larvae:- 0.1351 [..'s ::Wi2,.< NOEC _-- __ 0 Average weight per surviving l,*rvaei 0. "1 - 0 25 mgg/larvae LOEC . 300 ChV , 300 IC s* __ __!_
Species: Pinephhalesproinetas .PpKCICR Tcst Numilberý I ()ý) Survival and Growth Data Day Control 300 ma KCL/L 450 mg KCIiL _ __ A B I cITD F G H IWJK1L 0 I /0 /0 /0 /0 10 /0 '0 /01/0 (D /0
/0 /0 /0 0 /0 /0 c) /0 /0 /0 '0 2
/0 C) /0 /0 /0 10 /0 /0 /0 /0 90 io t'o (,0 /Qo 1O to ,'boic o o 9 4 1C /0 /0 /0 /0 /0 10 /0 C0 10 1/0 10 00 'C) 0 ,C0 9 9 6 0 to /0 /0 /0 Ib /1 /0 C /0 }/0 '9 7 1C~r
/0 /0 /0 /0 (o /0 /0 /0 /0 ,
A = Pan weight (mg) Tray color code:: k 4.b 1.eo l.b1-t<A.% 3;7o, I .0 43 W.O 1.o* 1 . A n a ly s t : /.1 f" 10 B = Pan + Larvae weight (mg) Analyst: ao o aa.0 0 o ao.o al.oq i1q.4i 1 a)I a.O ao.4l1 I l1B.91
,:R.{
Larvae ,eight (mg) = A - B 7.7'-* 7.57 b~~~~~~.*'.' .- U*% *9L U7L*,\ *.4 .5 Weight per initial number of larvae (mg) = C/Initial number of larvae
'N
-N Ax 4, V.
qz)> 1, 0* A (b"
'V ',V
\"
0 0" 131 0 Average Percent s'eight per reduction initial from control 1(.0, 0.~x ~ ____ number of (0/) larvae (me) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig unusually large, d&r decanted and returned, w = wounded Calculations and data reviewed"
[1j- WI II iihI M V dI 1 ItI~d~. 1 v~lj: j 1 - - -11. Species: PirnephalespromelIas PpKCICR Test Number. o0S Sur-vival and Growth Data A = Pan ,,eight (mg) Tray color code:: 66>-'* Is.33 Iq *2 lq'-39113.3'ý 1111-39 N31) 13.9t4 IN~SO ;ltq.Qu 1139c115iq An lvst: _t B= Pan + Larvae wright - (mag) An*alyst: IT 0 .- lq .r79 1(.5
- J '-/.q IBA() I*z .L1Lj 1.9 .9-51 19 ;0 S.Cziq jS* 15 .3 I.S .93 Larvae veight (mg) A - B Weight per initial number of larvae (mg) ' ,, A
= C/Initial number of larvae o' c. - 0 0 0 O o o '. O Average Percent weight per reduction initial from control OSc0 3\S -:. 0 number of (1%)
larvae (mag) Comment codes: c = clear, d = dead; fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg =,unusually large, d&r = decanted and returned, w= wounded. Calculations and data reviewed ., Comments:
Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimeph/alespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test nurnber: ý PpKCIC74 t 145 (4109 at 351 Depot St ) Test dates: Nonember 14-21, 2006 ,,,eeised by:
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.7n -t,. Mn-- 1 eN;thtyln-iA Coeff,,rT.6- ItY. r; A 10 10 13.16 204 0 7.74 0774 0 774 Contiol to0 10 14 83 22.20 7.37 0 737 0737 5.3 0 737 W000 0737 5.3 Not appht:r C 70 J0 1252 20 07 7 55 0.75S 0,75S t0 170 13.78 20.61 6 83 0 603 0 683 S10t 10 15.82 21.04 522) 0.522 0,522 300 F 10 t0 13.72 19 49 577 0 577. 0,6t9 135 0577 100.0 0.619 13.5 16.0 6; to 10 14.56 27.42 6.86 0.686 0696
- 11. 10 to 4.50 2 142 6 92 0,692 0.692 1 10 10 14.31 27105 6 72 0.672 0,672 450 .1 700o 14. 70 20 41 6.31 0611 0.652 8 3 0637 95.0 0.620 9.9 76.0 K to 9 15.67 22A 1 6.44 0 !16 0.644 1- 10 9 13 60 18.91 5.31 0590 0.531 M 70 7 13.34 17305 3 71 0530 0 371 600 10 8 14 82 19.85 5.03 0.629 0.674 20.5 0.503 10.0 0 009 70.4 3t.6 010 7 13.18 19.20 6.02 0.860 0,602 I' 10 8 14.37 19,79.5 542' 0 678 0 542 Q t0 5 13.38 71652 3-t4 0.626 0.314 750 R 10 5 14.74 77.94 3.60 0.720 0 649. T3 0.360 50.0 0.324 77 1 360 S t0 6 14.37 is 10 3.73 0.622 0.373 T 10 4 13.94 16.44 2 50 0-625 0 250 U 70 2 14.50 15 81 1.31 0,655 0.131 900 V 10 3 14.04 . .15.59 155 0517 0.659 15.5 0.55 7 200 0.126 8.7' 87.9 W 10 2 13.93 15.38 1.45 0.725 0,714 X 10 7 15 79 75.93 0.74 0740 0.07 I Durnnelt'5 MSD v3loe: 0.0987 MSD = Mnmitum Significant Diffetence PMSD: 1 3.4 PMSD Percent Minurtunu Significant Difference PMSD is a measure of test precision. The PMSD is the stinuint percent difference between the conutol anti neatrmn thai can be declared statisticalty s-tf, caot to a whole effluent toxicity te avetage. a s ignoficant difference occurs for Eovirruivoeeta I resting Solutions. Inc. cbson mc toxicity tests wheir a toxicant reduces Pi-nt-;hrles growth by I5 0% frori dhe connol (delernured totim re flertece toxicart testiog).
Lo-ee PMSD bound deteenrrtend by USEPA ( 10th percotrilc) 9.4%. Upper PMSD bound deteeemined by USEPA (901h percentile) = 35%. 1he lorer and upper bounds wtuce ctalcmtmled by the USEPA suing 205tests conducted ftort 19 laboratories for Putephales powth in chtonic refetenec toXicant tests. USEPA 2000. Understanding and Accoonting for Method Variabili*y in Whole Effluent Toxicity Applications Under the Natiorta Pollutant Discharge Eilmnatton Prop'amsr EPA-833 R-00-003 US Environt-ental Protection Agency. Ciocuionati, OH t 0rg.nttnsrs ohlbocd,'fomt Aqurtie sioSystcors. nc I 1. 14 06
Environmental i esting Ol)iouuons, inc. Statistical Analyses Lae-aI Fish Groath and Sriv,.I T,,,t- Day S.u-i,nI StoctDate 1!114/2006 Te,,t ID PpKCICR Sample ID REF-RfToticorl End Dae 11,2112006 Lab ID ETN'.EoFr. Testing Sol Saxmpl, Type KCL-Potassntrn chlolrote Sample Date Pltrocol FWCHR EPA-821.R.02-013 Test Speeos ?P.PPtmephales promelas Commenlo Coon-rtL 1 . 2 3 4 D Cntrol 10000 1 0000 1 0000 1 0000 100
- 0000 I0000 10000 1 0000 450 1 0000 10000 09000 0 000 600 07000 18000 0.7000 0m000 700 0 5000 0 5000 0 6000 0 4000 900 02000 0 3000 0 2000 , 1000 Transform: Arrein Square Root Rank 1-T.iled Number Total Conc-metL Mea n N-Men Mean Mi3n Ml.. C'Vn% N Sum Critical Re-p " Nuamber D Cotool I 00(00 1 0000 I a120 4120 1,4120 0000 4 0 40 300 10o00 10000 I 4120 14120 J 4100 000'0 4 13.00 1000 0 40 450 09500 09!00 1.3305 1 2a90 j 4120 70712 4 1400 1000 2 do40 600 0-7500 0 7500 1 0492 0 9912 1.1071 6 183 4 10.00 000 10 40 "700 00000 0.0000 0 7164 0.6947 0 861 10067 4 10.00 1000 20 40 900 0 2000 02000 04502 0.3218 0.5796 23087 4 1000 10.00 32 40 A aai i TestU Staw,.at Critial Skrn Ku,-,
Shtpooo-Wok's Test id-1tcas to no dia itotbato,, (p > 0 01) 0.927304924 0 834 -0. J17**462 -007299076 0 Equabiy of wamýae ca*not be coot U0vmd H-1oýTthraTnoi (1-tail. 2.05) NOEC LOEC ChV TU Steers Mauy-One fPard 0est 450 600 519 615242. Tteahnents vs D.Contiol MNl*timum Ltkelihood-Probit Pr-neter V.l[e SE 95% Fidaria Le..tsL Control Chi-Sq Critecal P-1-ala, *i Sigitta leer
ýSlope. E 18545127 I 18554237 5894832038 1054220822 0 0.5106372-51 7014727783 092 2862395394 0.121676037 3 Intt:cept -13 5247007 3 723327760 -2. 1364463 -II 9130032 TSCR to Point Probilo nxg/l. 95*/. Fidaci.l Limiu, 09 "
ECO0 2 674 379 6073413 295,2835229 439 0208562 EC05 3.355 459466902 383 559901 5[1 8507084 " ECIO 3 71 50806994638 440 1419331 556 5132353 07 ECi5 3.964 504 8617129 432.2758146 589 6651074 EC20 4 158 575 4*70324 517 9312937 6132379399 0.6 EC23 4326 603.012277 5498515822 644.7448404 005 EC40 4 747 678 5301092 633,44930993723 2887003 04 04 EC00 5000 72844?7003 683 7392443 781 89-10101 EC60 5.233 7380268659 7T31090911 850,8899764 0.3 EC75 5 674 879 9634d94 814 8968753 939 3037695 0.0 . I 0 EC60 5842 922 1477985 8480480517 1052.A48559 EC05 6 036 973.706028 8876041206 1132 238219 0 1 . EC90 6282 I143.106519 939 1191681 1142,443569 00 gc605 6.645 1154174127 1019754797 1427556739- 1 1o too 1too 0000 EC99 7 326 1397,83602 1137.558721 18560453020 Dose mg/C Do. e-Il p o n sPlot 0.3 0.7
- 0.6 0.5 03 0.2 071 to to
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Environmentai testing Solutions, Inc. Statistical Analyses Lýt-na Fiah Gio1ht -n6 Soo' caI Tnot- Da, G-na'02 S&tDain J f1112006 TutI D PpKC ICR Sartrp),ID REP-RerTomcar~t End Damý. 1121 /2006 Lab ID, EmSE ntw..T~stotg Sot Sooplpi Typ,: KCL Pctasotor Ohlond, SoanplnDatnc Protocol. Fwc HR EPA 821*R-02013 Trsl Spnoos PP Pmunrphln ptoxmmnb Cortttn Cot Con.mg/L I 2 3 A D-Cortlol 0 7740 0 7370 0 7530 0 6830 300 0 5220 0 5770 06860 06920 450 06720 06310 06440 0 5310 600 0.3710 0 3030 06020 03420 750 03140 03600 03730 02500 900 0 1310 0 1550 0 1430 00740 Trana form. Ijatlnanformned 1-Tailed hotoni, C-n-rnmlL Oman NIl -an l ran Min lM., C'N/ N -Sotl Critacal 0,01SD M .n N-Nin D-CotItot 0 7373 I 0003 0 7373 0 6030 0 7740 5 316 4 0 7373 1 0000
,300 0 6193 0 3399 0 6193 0 1220 06920 13 303 4 2'03 2 180 0 0937 0 6194 0 8401 "4 0 06195 0 8O03 0 6193 0 5310 06720 9 916 4 2.600 2 180 0 0987 06194 0 8401 600 05045 06843 05045 03710 66020 19000 4 0 5015 0 6843 750 03243 0 4398 0 3243 0 2300 0 3730 17 146 4 03213 0.4398 900 0,1263 0 1712 0 1263 00740 0 1550 28 671 4 01260 017!2 A u-ilh" T.U SSa tiý 6 Critical Sk-c Kumo Shapito-Wlas Test jodltalt, ..... aldznbutn (p > 0 01) 0 924493b49 0.805 053262068 0.93050657 Boo~lr~ersTcl indicates coual nans to (p 0.30.) . 1 389453173 9 2103405 HyathtTaasT I(-toi0.05) NOEC LOEC Chv TU MiSDu IlSOp %ISB MOSE F., Ob d8f DtonnmorTcsl <300 300 0 098731113 0 133918094 001S526033 00O4102278 0.C0A364.2091 2 9.
Ttcaoontsrs DConol Lin-r Inutrpolation (1200 R-maplco) Point mg/L SD 95% CL(Enp) Sk-c DC05* 93 82 47.73 51 72 316 70 24121 ICi0° 187 64 73 50 103 45 609695 1 4 17 ICIS" 231 5 9811 15517 615.17 0 11327 t 1C20 48862 75 26 138 39 612517 1 2522 09 1C23 536 75 42 15 421 08 668 52 02086 IC40 651 72 2945 52479 71672 1 1008 0 C050 713 07 20 57 644 68 778 29 -01493 07 otdjlco IC es Son US, Iowtl ctoncmnrationt ISstttal*t
- a06 003 04 0.3 61 00 0 200 400 600 800 1000 Dose mngFL Dos c-Rct¢pensc E[la 0.
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to 0 Organ/tw obtaindfrom Aq-icat BoSptecot 1n-5.3. 1.11-06
Species: Pimephales.promnelas PpKCiCR T,--st Number. \ c)S Daily Chemistry:. D2ay O 1 2 Concentration* Parameter"** t*. CONTROL pH (S.U.) ."- .9 . ".3".* . - DO (mg/L) k**.2- 6. "..,z"-. Conductivity prnthos/cm) .3.P Alkalin it v (mg CaCOJL) Hardness Tfemperature 1 -S 1ALA ( °C ) "-2 q " q . . .*- q zq - L-pH (SU) 9* ".3' ý."-3-I 9.5 4'/ " 300 mg KCI/L Conductivity nmhos/cm) Temperature I i/Sb ( HC ) q ._ -.LA PH (S.U) -4,..1. 5Q2 DO (mag/L) K.Coductivit2y (Prnhoslcm)0 Temperature A 1 - . t,. ( °C ) zq . pH (S U -) q-.*9-.62 D9.5 - 3- 4-.5 q-5 1 9.31 DO (mg/L) 6. ~ g..2 C9: Z-600 mg KCKL Conductivity ; 900 mg KCI/L Conductivity/D 013 (prrmhos/cm) H ID .0O DO (rag/L) iFniemperanure (nitia. ." )30:0gl 9.Z{3 Finl Q 7500mg KCI/L ( hs/mConductivity 'v* i'*" *.%" Temperature"" . .
~(oC ) "4 j - q ¢ 2 qq . ' c ..
PHiia U)a l, 1-4nta qia2nta 5inl1
Species: Pimnephalespromelas PpKCICR Test Numberý 10'-- Day 3 4 6 Analyst t-V-A-v Concentration Parameter T CONTROL pH (S.U.) 55 2 )1 DO.(mg/L) .0 fl.2 Conduct i Iity ( mhos/cm) Al kalin ity (mg CaCO5/L) GO Hardness (mg CaCO1 /L) I3 Temperature (°C) "z l "q 2'A."1 '.2A. "t.A].'4',: A. S 2q. pH (S. U.) L.,,-, q.2Z ,g":- 2 q .3 , 9 DO (ag/L-) ."2. * /. t.. 300 mg KCI/fL Conductivity ( mnhos/cm) e Bze (oC) pera tu re Tern
?. I 7 -'A.< zA.R .*
-qq2qL -!.q,. ..;. L4q(o pH(S.
U.) ")..a 926 2 .-19 23 1 4".0 W,3 q.e6' r .4 DO (rag/L) (0." oqQ (f: o,-4 .63.0 .2. 450 mg KCI/L Conductivity (pmrbos/cm) 1)1 Temperature (°c) -7.1 zq. Z J,& zN. "2q 2iq - 2zq3 I pH (S.U.) *'.. -4 21 4..0 22. 600 mg KCIL .DO (m/L) '2. q .6 U.6 ". .6 . Co nd uc tivityyý) (rnhos/cm) ' ?C T em perature 24 .1 7 zq.q * -qq.1 ý.) 2-.. z - (0C) PH (S.U.) "q,* .3Z 21. -. 3 2- q.'qq 9.5"0 I9eq9:r'. DO (rn* L) -".. 2, 750 mg KCIiL Conductivity HED (Lrn-hoslcm) Temperature zqq ZQL *?'1 71 (-C) pH (S.U) ?,-70or ,39 as*<,3
- .o'30957 )5 DO (mg[L) % . * ** m :* 61* q-900 mg KCI/L Conductivity (n-Lhos/cm) 19? 1Q4 12-0 o Tem pera tu re, .7 "zq.
STOCK Conductivity Initial Final Initial Final Initial 1 Final Initial Final
Ceriodaphn ia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 USEPA Control Limits (1 2 Standard Deviajions) 1.10 K 1.08 K 1.06 1.04
'?Z. 1ý ,i SI II I I I I I I I I USEPA Warning and Control Limits (75 and 90th Percentile CVs) 2.0 1.5 I1.0 H 0.5 K II I I I I -l I I I i i I i I i 1.4 LaboratoryI I I I I a I I I I C Is Laboratory kWarning and Control Limits (10Odnd 25; Percentile C/s,) .
1.3 1.2 1.1 1.0 0.9 I I " I 1 I 1 I ! I I I P I 1 0.8 ý,b iýb __ýý6 Z(, Zb Z(, Qý, Jr &ý
ý,!V Z'ý b, Z(, Cl%- c3b C31, Zb ý1_ Zb 11 Z6 ý3!1" (,b
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z z&- Z'ý' Z61", Z.0" Z-031 "q .N'L Nz- ý,*% Tjest date 7-day IC 25s 25% inhibition concentration.. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population. Central Tendency (mean IC 25 )
... Warning Limits (mean IC 25 +/- SA10 or SA 75)
... Limits Contol imis (mean IC2 +/-Control S S or 2 Standard Deviations) (man C25 A 25' A,90'
Environmental Testing Solutions, Inc. Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Control Chart' using Moderately Hard Synthetic Water State and USEPA L2bora.fory Laborwor-y USEPA USEPA Test number Test date 7-a2y ICs CT S Coitrol Uinits Sý_0 War-ung Linats S..S Contii Linits -SVs Wanhiug Linits S&90 Control Linits (g/L NACI) (g/L Nac) CT-2S CT÷2S C:r"-S,, 0 CT-S,., CT - S, CT 4 S,,, CT - S,,, CT+ S,,, CT - S,, CT+ S,ý0 1 06-07-05 1.05 2 07-12-05 1 06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.19 0.88 1.24 0.48 0.58 1.53 0.66 0.40 3 1.71 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0 18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 4 08-09-05 1.08 106 001 1.04 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 5 09-13-05 1.04 1.06 0.01 1.03 1.09 008 0.97 1.14 0.18 0.88 1.24 0 48 0.58 153 0.66 0.40 1.71 6 10-04-05 1.09 1 06 0,02 1 03 1.10 0.09 0.98 1.15 018 0.88 1.24 0.48 058 1 54 0.66 040 1.72 7 11-01-05 108 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.25 0.48 059 1 54 0.66 0.40 1.73 8 12-06-05 1.04 1.06 0.02 1.03 1.10 008 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1,72 9 01-10-06 1.08 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 088 I 24 0.48 0.58 1.54 066 0.40 172 10 02-07-06 1.07 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 11 02-07-06 1.06 1.116 0.02 1.03' 1.10 0.09 0.98 1.15 0 18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 12 03-07-06 1.05 1.06 0.02 1 03 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1 54 0.66 0.40 1.72 13 04-04-06 1.06 1.06 0.01 1.03 1.09 0.08 0 98 1.15 0.18 0 88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 j4 05-02-06 1 08 1.06 0.01 1.03 1.09 0.09 0 98 1.15 0 18 0.88. 1 24 0.48 0.58 1.54 0 66 0.40 172 15 06-06-06 1.06 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0 18 0.88 1.24 0.48 0.58 154 0.66 0.40 1.72 16 07-11-06 1.09 1.06 0.02 1.03 1.09 0.09 0.98 1.15 0118 0.88 1.25 0.48 0.59 154 0.66 0(40 1,T72 17 08-08-06 1.07 1.06 0.01 1.04 1.09 0 09 0.98 1.15 0.18 088 1.25 0.48 0.59 1 54 0.66 0.40 1.72 18 , 09-12-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 19 10-03-06 1.06 1.06 0.01 1.04 1.09 0.09 0.989 1.15 0 18 058 1.25 0.48 0.59 1.54 066 0.40 1 72 20 11-07-06 1.06 1.06 001 1.04 1.09 0.09 0 98 1.15 0 18 0.88 1.25 0,48 0.59 1.54 0.66 040 1.72 7 Note. 7-d IC,, -day 25% inhibition corcenltation. An estitiation of (he conccntrarhon of sodium chloridc that woold cause a 25% reduction in Cenodaphnia teproducfiori for the test populatioti. CT ý C~itTal toldincy (mean IC20). S = Standard deviation of the ICI, values Laboratory Control And Wandng LiniLs Laboratory control And warning Iimtits were established using lhe standard devialion of the IC2 values corrc'rpondlig to (lie 1 10th and 251h percentile CVs. These ranges arc more stringmit than the conhoul rod wari limits recorinictnded by USEPA for the test uuetltod atd midpoint, S = standard SIO deviation corresponding to (he 100 p ercentile CV (S,ý = 0.08) 0 Sý_5= Standard deviation correponding to thc 25a pcrcsuilill CV. (S., 0 17) USEPA Control and Wanintg LUnits S.75 = Statndard deviation corresponding to the 750 percenutile CV. (S1 1 = 0.45) 7 S&0= Slatndard deviation corresponding to lie 90 .percmitilc CV (S,90 = 0.62) CV Coefficiemt of variation of the ICI, values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole.Effluent Tovicity Applications Under the Nation*l Pollutant Discharge Elinination Program. EPA-833-R-00-003. US Env'iroiinmctal Protection Age*cy. Circ nuaat 10,6.16
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test Test date Control Control Mean CT CV CT MSD - PNISD CT number Survival Reproduction for Control Mean for Control ()(oH sprin g/ferrrae) Reproduction (0/) *Reproduction (%) for PN1tD (%) (offspring/female) Cv (%) 1 06-07-05 100 304 5.0 26 8.5 2 07-12-05 100 30.4 30.4 7.5 6.2 2.7 8.8 8.7 3 07-12-05 100 31.1 30.6 7.2 6.5 3.2 10.2 92 4 08-09-05 ioq 28.3 30.1 7-3 6.7 29 10.3 9.5 5 09-13-05 100 27.9 29.6 7.3 68 '3 9 i,39 10 3 6 10-04-05 100 27.0 29.2 5.8 67 30 11.1 105 7 11-01-05 1oo 28,4 29 1 10.3 7.2 38 13.2 10.9 8 12-06-05 100 326 295 63 7.1 23 71 104 9 01-10-06 100 29.2 295 4.8 6.8 2.3 78 10.1 10 02-07-06 100 307 29.6 6.0 .67 24 7.9 .9 9 02-07-06 100 29.9 29.6 6.8 6.7 2 2 7-5 9.7 12 03-07-06 100 28.8 29.6 5.9 6.7 26 8.9 9.6 13 04-04-06 100 6oo 27.0 29.4 52 6.5 2.1 7,6 9.5 14 05-02-06 28.6 29 3 8.6 6.7 2.7 93 9.4 100 15 06-06-06 30 3 29.4 52 6.6 3.0 98 9.5 100 16 07-11-06 29.0 29.4 5.4 6.5 25 86 9.4 100 6.7-17 .08-08 -06 28 6 29 3 8.9 3.7 128 9.6 18 09-12-06 100 30.9 29.4 54 616 3.3. 106 97 19 10-03-06 100 32.3 .29.5 4.6 6.5 3.1 95 9.7 20 1 1-07-06 100 310 29.6 6.3 6.5 24 78 9.6 Note. CV = Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.5% in Env'ironmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity tests. Lower CV boutid determrined by USEPA (10 'ý percentile) = 89%. Upper CV bound determined by USEPA (90 ' pcrcenole) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difierence between the control and treatrnent that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces CeriodOphniC reproduction by 9.6% from the control Lower.PMSD bound determined by USEPA ( 10 'h percentile) 11%. Upper PMSD bound determined by USEPA (90" percentile) = 37%. CT= Central Tendancy (Mean Control Reproduction, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories fur Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in 'Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Prograrm. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 11-07-06
Ceriodaphniadubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 I I I ~ -.-...I-.- F 35 30 25 20 15 Minimum Acceptance Criteria (> 5.0 offspring per survivingfemale) I rI I I I i I II . I 0 40
~0 0 30 0 20 0
(-) 10 0 40 I I I I [ I I [ I I I I I I
- 1 1 1
[ USEPA Upper PMSD Bound (9 0 1h percentile < 37.0%) 30 20 I0 I.......I I I .*I I I ~I IIso (A k] Test date Control Reproduction, Coefficient of Variation (CV),between or Percent Minimum Significant (PMSD) PMSD is the minimum significant difference the control and treatment thatDifference can be declared. statistically significant.
-- -- Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadubia CdNaCLCR 9: 5n Dilution preparationinformation: Comments: NaC CHM number Q...* \7-Stock preparation: 100 g NaCHl (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mE/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1 1500 1500 1500
!500 Test organism information: Test information:.
Organism age: < 24-hours old Randomizing template: C-Aý .e_ Date and times organisms t'4-os.--.o*- , --cO'Oc2,- Incubator number and were born between: shelf location: Organism source: 1o.3\-oo6A o ' < t,,o to3, r'5 zo YCT batch: ______-_ T.ransfer bowl information: pH =-.83 SU Temperarure =l,.CC Selenastrum batch: ___."z,-,i_,, Daily renewal information. Day Date Test initiation, MHS water Analyst renewal, or batch used termination time 0 1- Oi-o- \\if1 0 _o12- c 2 1 0qo--.0 1C) 7- 0 6" 3 2-10 1D-,ooC (3-,-A I12 L)-2:1-o*0 IO2--Lq C2 5 ll-la_060_____;s__ 1y47 6 It-,il-o* -4'-_
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L 6l Control information: Acceptance critena Summary of test end oints.- % of Male Adults: '. - 20% 7-day LCS0 > "Atoo % Adults having 3 'd Broods: /m7. - 80% NOEC. I CoO 0 7,, s 20% LOEC _k -.O__1 % Mortality: Mean Offspring/Fernale: .131, 0 > 15.0 offspring/female ChV I (s -. %CV: "40.0% <.7. IC25 : I. ,. 0
l V I II I C i~ t i l I I ý* tlIII
- I I~ ? t Species: Ceriodaphniadubia CdNaCLC.R : S:
CONTROL Survival and Reproduction Data Replicate number Day 2 3 [ 4 . 8 9 10 1Young produced 0~rIQj 1 C) 2 Adl mortality
,Adult mortality)___
Young proý dudT f L ___ _ ___ __
'Young produced L __ . g, ._ 0 .__
Adult mortality ___ young mortality [AdJult produced J_ _ _ _ _ I0 "1 k t 2 6 Young produced k~~~~{o Total young9 produce d b C -\). ~ ~
?2. 2 Final Adult M ortality _ . " \ . . L * ' ...
X for 3'* Broods j ".t *-.-- " "- " . * >-" Note: Adult mortalhr' (L lve, D = dead) Concentration:
% Mortaliry:
- 07.,
Mean OffspringFemale: 3*.0 600 mg NaCI!L Surnival and Reproduction Data Replicate number Day 1 2 3 4 j 5 1 6 7 8 9 10 2 I Young produiced Young produced C') O .) 0 ( ~1C0__ QIc
) 0
- 00!
0o C Adult mortality , . u .. _ _ * , .. xJ .,_ 2 Young produced )o01L _*__*____ .o_ 1 Adult mortalitey J______ 'J-'-.'--~ _ 3 SAdult_ Young produced m raiy Ad'0 h " 5 Young produced Adult mortality ._ __1 Ik._ _V_ -I-1._I_ _ __ -I 6T Young produced L2jI -L C)1 1 I0 0 __ Adult mortalirvty j_ 17 I V - 7 j Young produced ~ 8 iA iS '~ S Ii.1I~ 7 Total young produced 33 2-*I
' Z a\ s 2.- .o0 Final Adult Mortality . ,.
- _ * ._ . t..
Note: Adult mortaliry (L live. D = dead) Concentration:
% Mortality: (",
Mean.Offspring/Female: ,.311
% Reduction from Control: 31-
Species: Ceriodaphniadubhi CdNaCLCR P: 800 mg NaCVL Surx.'ival and Reproduction Data Replicate number Dav 1 2 3 -4 5 6 7 8 9 10 I 2 Y¥oung Young produced poduce 0f Adult mortality 0_0__c) 0l0 cIc)0t101°' 0__CC 3 Yung produced 1C )[ O (22 ) .. C)' 0 r 2__ Z l] ortU it k_ k, __ k.__. k. _ ___ Adult mortaflry L_-1 L- 1_o f*-" ____ L__ ai_. produced Adult mtoung _ ('4___ 5 LAYoung produced ] , { Tv I[ , 1"I 6 Young produced 1 ____ g~- c D1-~]~1"L~I--c fl~~Autmraiyj~[ c)'T 7 Young produced L-I LA llnFSI 19 1 1,i* Total young produced 3 2 2 Z 5- "-' Z 3 0o 32 3 0 23 Final Adult Nlortaliry J 1... . L [ . Y... . . Note: Adult mortality (L live D dead) Concentration:
% Mortialiry:
Mean Offspring/Female: 31.0
% Reduction from Control: 0%
1000 mg NaCLL Survival and Reproduction Data Replicate number Day _________ 1 2 3__4__ 6 7 'S 9 10 I Young produced c___0___c Adult mortality IL_ L- IL -_. L 3 oung produced 0 0 L- i.1 - La I - *- I-3 Young produced Adult mortality J____ __ ___] k__ ] O ~ ~ _~ ~k_ c IC Adult mortality j. .[ J _...'I l __._ 4 Young Y produced Youngpr-odffced
,_Lq ltfl.-
/-- ______ ____
10-- _5_____ 1 1 17- v. .
,*- 0[.
Noe [Ad ult mortality=hIe, JL D = dead-YoCoung produced 0,nraon 0 Finl [ A dul [Adultm6' mraI ortali], t I v.. .. _-.i._t- ___ Z___ J. L.__J \-- L._._iT ,i_ L._.F... 7___ Young produced 14ýA Si 3 1 1~-h '1-7 1 Total young produced 2O \{ i 3~z z~c Final Adult Nvlortality [ L. . "- kj Note: Adult mortality (L live, D =dead) Conc~entrati.on.
% Mortality: 07.
Mean Offspring/Female: 3o. 5
% Reduction from Control:
Species: Ceriodaphniadubia CdNaCLCRR #: 5S 1200 t11a NaCI/L Survival and Reproduction Data Replicate number Da_ _ i 2 3 4 5 6 7 8 1 9110 I Young produced C O) L 0 0 l)O'Q
.Adult A mortality _ _ _ i__._ t,_ ti _
2 NYoung prodiiced o C) C)f**N1 F Adult mortaliry j ie [ B
*--i kQ_ ,.- _ ,_ 1 'IT Young produced _______ 0 0_ I in"_
Adult mortality (L =Iv___=_dea_)_- Young produced" r Adult younY, gp,-,. mortalityt d=uc d -2" , . it.LA:. : 6 Young produced _____ 0___ 0___ Ung produced C) 0o (.0 0 l H
%oa e o -0 .
Total young produc'ed I , ItO AdanmoOffspr __n___ __a___: ___ FinalAdult N lortalir,, - i. . . Note: Adult mortality (L liv, D deud) Concenrration:
%dull Moolaliy o7.
Mean Offspring/memale_ .1
% Reduction From Control: -1o. V.7, 1400 mg NaCIfL Survival an Reproduction Data
__________________________ Replicate number a2y 2 3 I4 5 {61 8 9 O Adult mortality I0{ _ ll.- l,"_ Young produced 0 O (O1 0 Adult mortality J - ~n 0 C) I Young produced 0 C-)- C) / f'-C)~ 0' 4oa Young produced -1 -I O Q O T 1 o
"{ ,fzf Adult mortality \7._
t~ 71~ 0_) 0 t I .__ 5 'Young prdced 6 jYoung produced ~ 7 jYoung produced 0 C) T Total young produced 17 -: D2 Final Adult Ntrtlir . '-~u '.. ~~L Note: Adult monality (L =Ive, D = dead) Concentration:
% Mortaliry:
Mean Offspring/Female: Z ,S
% Reduction from-Control 1.q,
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LAI 'IVIUII lIIJLI till UNtIL-* OUJti0lUll1h 1i1u. Statistical Analyses Ceriod.phni. ur-l -d Reproduction Test-Reproduction Stan Date l If7/2006 Test ID. CdNOCCICR Samrple ID ,EF.RcrfToo,cant End Date 31/14/2006 Lab ID ETS-Eucsr Tcslmg Sol SampIo Type. NACL-Sod:um chloride Sample Date Protocol FWCHR'-PA.82 1-R-02-013 Test Species. CD-Cenodaplmcno 3,ba i Cortrneats. Conc-utl/L 1 2 3 4 5
- 6 7 8 9 30 D-Conuol 32 000 33 000 30 000 31 000 29.000 34 000 32 000 32 000 28 000 29 000 600 32 000 34 000 29000 3 000 35 000 33 000 30.000 3 000 32 000 32 000 S00 32 000 29 000 33000 32 000 30 000 31 000 32.000 30 COO 33000 28.000 1000 34 000 27 000 29.000. 29 000 31 000 33 000 31.000 29 000
- 32 000 28 000 1200 13 000 8.000 10000 8 000 12 000 16 000 4 000 6 000 8.000 6 000 1400 7 000 3 000 0 000 0000 1 000 4.000 3000 2 000 4.000 1 000 Tii ns-oen : Un tr.norrned I-T.iled Isototic Coso-eig!L ]e.nn N-N- Mi Mi "I Mer CVi%'o IN -St.t Critical OISD MeJs N-Niece D-Coontc' 31 000 3 0000 31 000 26 000 34.000 6 270 30 31 350 3 0000 600 31 700 1 0226 31 700 29 000 35.000 . 5 95R 30 -0.662 2 237 2 437" 31 350 3 0000 8o0 31 000 1 0000 31 000 28 000 33 000 5 483 30 0 000 2 287 2 417 31 003 0 9888 3000 30 300 0 9774 30.300 27 000 34 000 7 469 10 0 662 2 287 2 417 30 300 0 9665 M200 9 100 0 2935 9 100 4 000 16.000 40 276 30 20 721 2287 2 417 9.100 0 2903 1400 2 500 0 0806 2 500 0.000 7.000 86923 30 26 966 2 287 2 a17 2 500 0 0797 Asiii/cry Tests Stotitl, Cntic-l Sket Kun Kobnogrrv D Test ndfcoaes normal dinbuthlo (p > 0.03) 0 81748134 1 035 0 44619717 0.3712333 Ronlerl's Test indcates equal 'asaoces (p ý 0 18) 7.55277777 15 0862722 Hl,3pothltei Test (l-tuit 0.03) NOEC LOEC ChV TV.J MSDu MSDp MISB M9SE F P'ro dOf Donver 's Test 1000 1200 1093 44512 2 41677734 007796056 1738 96 536588519 1 6B-38 5334 TreaonentLs vs D-Control Liin.r lntetpol.tion (200 Retes ples)
Point ig/L SD 95% CL Skte IC05 1004 88208 51 0038904 825 634017 1014,14902 .2 6889 [CIO 101966981 3 96832498 1007.34438 1028 34195 0 3117 3C15 . 1034.45755 5 61835965 1023 38686 1343 09093 -0.2720 10 1CoO 1049 24,28 -5 40692453 )038,23354 105791483 .0,2188 0.9 1C2S 1064 03302 5 350467A3 1052 85837 1072.74883 1422 1109 39623 609494531 3095 59506 1118 42537 02053 IC40 IC50 11379717 717272292 1323 85682 115346069 043336 07 0.6 0. 02 0.1 0.0
-0.3 0 500 1000 1500 Dose mg/L Dose-Response Plot 40 305 00 1-tail 0 05 level ol signflicance o 25 "r 20 o 0 0* 0o 03 11-07-06
Species: Ceriodaphniadubia CdNaCLCR I-_SS Daily Chenzistrv: S Day A sI t I Concentration Parameter " **-7,
- pH (S U) 1?, 2 9q .9 90 .? .
DO (m!,/) , .9 .".*. CON.TROL Conductivity ' e , (Prrmhosicmn) 3 z 12-(rnlg CaCO3/L) °.o/ Hardness (mag CaCO-,/L) 'qg Tempera .ture [ z p H (SU.) 9.0 -4,:
.q "2& 973 " ".T
- DO (rng/L) .7 .3 .-6,a*
0 600 mg NaClI/ Conductivity (pmhos/cm) /510 " 0-0 q 0O pH (S.U.) 9.O "q .0 9.% 9.o .o4 ) .q DO (mg/L) q, q . . . 800 mg, NaClgL Conductivity (Hmhos/cm) Mq9ID I 0 Temperature IS q9 1(.7 pI- (S.U. 95 .5 '*,q 2- 9.)J . q.-'Y DO(rag/L) q* *.. *9q 9e ~ 1000 mg NaClfL Conductivity * (HMhos/cm),.. 0*- Oa 0 Temperature 7q. 71.; ] q q - . ,q.L.G -Za
,. (0c).
..~~ pH (s. u.) , .<1 q ,. .5 '. a 1200 m g NacI/L Conductivity Temperatur-e
(°C) ,6 7gl 2qN; 5 Lqq. I
' ~ ~~~~~
pH (S.*U.) ",0 "(1 QQ .~ )* Q* DO (mg/L) 90 ,. -( ).9< 5,Z 1400 mg NaCVI,, Conductivity (,*nos/cm) 3WJl 30coo o-Temperature -q z .( Initial Final IInitial Final I nitial Final
JLu I I IS
. I ~ i S A I.n ------ 1 .
Species: Ceriodaphniadubia CdNaCLCR *: 55 Day 34 56 Analhyst 4-- Concentration Parameter pH(S.U.) Io DO (m aL) (Y CONTROL Conductivity (pmhos/cm) 361 C12-Alkalinity (Mg CaCO 3 /L) Hardness (mg CaCO;/L) Temperature Z 'k. (0C) 600 mg NaCVL, Conductivity (pmhos/cm) H20 Temperature IX.-L (oC ) .z-pH (S.U.) 9 S "o1-DO (m 9/L E2 1000 mg NaCLI1L Conductivity ( mhos/cm) 102q eo DO (mn Temperature q .L1I - A / k(S.U) . S. '7 "5.© 9 Temperature 2~1?I 1200 mg NaCIL (0C) Conductivity pH (S.U.) .3p94 1200 rmg NaCIIL Conductivity (-C) 2 (pmhos/cm) q 0 (0U4 STO ngNCK L Temn peratu reFia Conductivity I n Fa I I (p~nLh os/cm) I CU
)
Initial Final ntil Final nial Final Initial ,.Final
January 4, 2007 Ruth Ann Hurt;.SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORiNG, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2006 Attached are two copies ofthe subject report for submission to the stateof Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected November 26-December 1, showed no toxic effects to fathead minnows or daphnids. The resulting IC2 5 values for both species were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests. In addition to the routine compliance test, fathead'minnows were also tested in Outfall 101 and intake samples which were treated using UVNexposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in. previous toxicity testing at Sequoyah. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist, Environmental Engineering Services- West CTR 2L-M Attachment cc (Attachment): R. M. Sherrard, PSC IX-C Files, RSO&E-EDMS-Muscle Shoals SQN November 2006M-second test
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: January 4, 2007
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Penmit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 1579
- 7. Receiving Stream: Tennessee River (TRM 483.6)
- 8. 1Q10: 3491.
- 9. Outfall Tested: 101 10.. Dates Sampled: November 26-December 1, 2006
- 11. Average Flow on DaysSampled (MGD): 1528, 1493, 910,908, 911, 915
- 12. Pertinent Site Conditions:
Towerbrom 960 (chlorination) was injected from November 26 - December 1, 006. The dates and times for the Towerbrom 960 injection are in the following table. Iniection Location Date/Start Time (ET) Date/Ending Time (ET) Essential Raw Cooling Water (ERCW) Train A&B . 11/26/2006 0000 12/01/2006 2400 11/26/2006 0000 11/27/2006 1600 Raw Cooling Water (_CW) 12/01/2006 1142 12/01/2006 1600
- 13. Test Dates: November 28-December 5, 2006
- 14. Test Type: Short-term Chronic Definitive
- 15. Test Species: Fathead Minnows (Pirnephalespromelas)
Daphnids (Ceriodaphniadubia).
- 16. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 17. Permit Limit Endpoint (%): Outfall 101: IC2 5 =45.2%
- 18. Test Results: Outfall 101: Pimephalespromelas.ICs.> 100%
Ceriodaphniadubia. IC 25_> 100% UV treated Outfall 101: Pimephalespromelas: IC 2 . > 100%
- 19. Facility
Contact:
Stephanie Howard Phone #: (423) 843-6700
- 20. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 21. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 22. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 23. Notes: Outfall 101 samples collected November 26-December 1, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC2 5 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant differences from the control during this-smdy period. Growth in minnows exposed to intake samples was significantly lower than minnow growth in the control.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. 2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received (°C) (mg/L) First Used By 112/605 to .11/28/06 1559 101 1/26/06 0753 11/27/06 1400 3 .7 / 3 .4' <0.10 11/27/06 0653 11/29/0.6 1507 Intake 11/26/06 0809 to 11/27/66 1400 1.4 <0.10 11/28/06 1559 11/27/06 0709 . 11/29/06 1507 11/28/06 0826 to 11/30/06 1510 101 11/29/06 0726 11/29/06 1400 4.3/4.6 <0.10 12/0.1/06 1513 Intake 11/28/06 112/673 0843 to 11/29/06 1400 1.1 <0.10 11/30/06 1510 120/653 11/29/06 0743 112/01/06 1513 12/02/06 1507 101 11/30/06 0758 to 12/01/06 1350 4 . 5 / 5 .2' <0.10 12/03/06 1511 12/01/06 0658 12/04/06 1512 12/02/06 1507 Intake 11/30/06 0813 to 12/01/06 1350 2.5 <0.10 12/03/06 1511 12/0 1/06 0713 12/04/06 1512
*TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperatuire was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1..0 2C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart!-UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes, 3
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aciuatic BioSvstems. Inc. In-house Cultures
- 2. Age: 24-26 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10. I
- 6. Test Initiation: (Date/Time)
Outfall 101 11/28/06 - 1559 ET 11/28/06 - 1042 ET UV Treated Outfall. 101 -11/28/06 - 1547 ET
- 7. Test Termination: (Date/Time)
Outfall 101- 12/05/06 - 1600 ET 12/05/06 - 0"954 ET UV Treated Ouffall 101 12/05/06 - 1545 ET
- 8. Test Temperature: Outfall 101: Mean =.24.62 C Mean 24.82C (24.1 - 25.l C) 2 (24.5 - 25.2-C)
Test Temperature: UV-Treated Outfall 101 Mean = 24.72C (24.1 - 25, I-°C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory, in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were-analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted November 28-December 5, 2006 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - da s) (% Effluent) 1 2 3 4 .5 6 7 Control o00 100 100 100 10.0 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 98 Intake 100 100 100 100 100 100 100 Test Solutions .Mean Dry Weight (mg) (% Effluent) (replicate number) 1 2 3 4 Mean Control 0.707 0.726 0.706 0.682 0.705 11.3% 0.644 0.641 0.682 0.601 0..642 22.6% 0.668 0.650 0.667 0.692 0.669 45.2% 0.649 0.745 0.637 0.677 0.677 72.6% 0.600 0.618 0.628 0.588 0.609 100.0% 0.536 0.595 0.571 0.666 0.592 Intake " 0.614 0.593 0.615 0.637 0.615 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2% Permit Limit: 2.2 TUc 95% Confidence Limits:
- Upper Limit: NA Lower Limit: NA
*TUa = 100/LC5 0: TUc = 100/IC 25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity.Test.
(Genus species) (Type / Duration) Conducted November 28-December 5, 2006 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions ' ('%Effluent) 4 5 6 Control 100 100 100 100 100 100 100 11.3% 100 100 1.00 100 100 100 100 22.6% 100 100 100 100 100 100., 100 45.2% 100 100 100 100 100 100 100 72.6% 100 100 io 100 100 100. 10.0 100.0% 100 100 100 100 100 100 100 Test Solutions . Reproduction (#young/female/7*days) Tt Eflueont) Data (r"eplicate number)
.(%_Effent 1 2 3 4 5 6 7 8 9 10 Mean Control 25 27 31. 28 28 27 28 29 29 30 28.2 11.3% 26 28 32 29 28 32 33 28 29 29 29.4 22.6% 29 30 29 32 29 31 30 31 31 31 30.3 45.2% 31 32 31 32 29 28 30 30 32 29 30.4 72.6% 28 35 31 30 28 34 31 36 30 28 31.1 100.0% 31 31 30 33 30 36 30 37 29 32 31.9.
IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 45.2%/., Permit Limit: 2.2 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa i00/LC5 0: TUc = 100/ IC 25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration) Conducted November 28-December 5, 2006 using water from Intake Percent Surviving Test (time interval used - days)_.___ Solutions 1 2 3 4 5 6 7 (% Effluent) _ 2 3 4 *5 _6 7 Control 100 100 100 .100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions -Reproduction (#young/female/7 days) Test Soluetios Data (replicatenumber) (%Effluent) 1 2 3 4 5 6. 7 8 9 10 Mean Control 28 25 25 28 30 26 30 28 28 28 27:6 Intake 32 28 31 33 29 28 32 30 31 30 30.4 IC 25.Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit:N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = 100/LC5 0: TUc = 100/IC 25 7
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) I (Type / Duration) Conducted November 28-December 5, 2006 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 . 100 100 100 100 100 100 11.3% 100 100 100 100 100 100 100 22.6% 100 100 100 100 100 100 100
.45.2% 100 100 100 100 100 100 100 72.6% 100 100 100 100 100 l00 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(% Effluent) 1replicate nu ber4 Mean. Control 0.565 0.645 0.669 0.763 0.661 11.3% 0.645 0.738 0.661 0.656 0.675 22.6% 0.597 0.602 0.630 0.709 0.635 45.2% 0.634 0.604 0.513 0.602 0.588 72.6% 0.542 0.583 0.583 0.630 0.5.85 100.0% 0.637 0.705 0.748 0.623 0.678 Intake 0.619 0.608 0.667 0.682 0.644 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA
*TUa = I00/LC%0: TUc 100/IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species .Date Time Duration -Toxicant Results (1C25 ) November 28 - Pimephales promelas Decenmber 5, 2006 1609 . 7-days KCI 0.63 .g/L Ceriodaphniadubia November 7 - 14, 2006 1127 7-days NaCI 1.06 g/L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Cheinistry Mean Values and Ranges for Pimephales proinelas and Ceriodaphuiactbhia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, Novem*ber 28 - December 5, 2006 Test Sample ID Temperature (C) Dissolved Oxygen (rng/L) pIt (S.U.)" Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (Vimhos/cm) (mg/L CaCO 3 ) (mg/1 CaCOj) Chlorine (rng/L) 24.5 7.7 7.3 7.76 7.17 310 58 89 Control 24.7 24.5 - 24.8. 24.2 - 24.8 7.6 - 7.8 7.1 - 7.7 7,59 - 7.90 7.03 - 7.35 300 - 318 58 - 59 86 - 92 11.3% 24.8 24.4 7.8 7.3 7.50 7.13 293 3 - 24.7 - 24.8 24.2 - 24.8 7.6 - 8.1 6.9 - 7.7 7.28 - 8.00 6.92 - 7.31 284 - 304 S 22.6% 24.8 24.4 7.8 7.2 7.49 7.12 '280 24.7 - 24.9 24.2 - 24.6 7.6 m 8.1 6.7 - 7.5 7.27 . 8.00 6.93 - 7.26 273 - 289
. 424.8 45.2%/i .. 24.5 7.9 7.1 7.47 7.13 255 24.7 - 24.9 24.2 - 24.9 .7.6 - 8.2 6.4 - 7.5 7.25 - 8.01 6.89 - 7.27 248 - 261 ,
24.9 24.4 " 7.9 7.2 7.45' 7.14 222
.6 24.7 - 25.0 24.1 - 24.9 7.7 - 8.2 6.3 7.6 7.23 - 7.99 6.88 - 7.27 215 - 234 -
100.0% 24.9 24.5 8.0 . 7.2 7.43 7.16 189 66 81 < 0.10 24.7 - 25.1 24.1 - 25.0 7.7 - .2 6.2 - 7.6 7.20 - 7.97 6.89 - 7.29 185 - 192 66 - 66 78 .- 86 <010 - <0.10 8.0 7.3 7.40 7.15 190 65 81 <0.10 Intake .24.8 24.5 . . 24.7 - 25.0 24.3 - 24.9 7.7 8.2 6.6 - 7.7 7.17 - 7.95 6.91 .- 7.30 184 - 192 64 - 67 80 - -84 <0.10 - <0.10
.24.9 7.7 7.7 7.76 7.36 310 58 89 Control 24.7 24.6 - 24.8 24.7 - 25.2 7.6 7.8 7.5 - 7.9 7.59 - 7.90 7.21 - 7.50 300 - 318 58 - 59 86 - 92 -
24.8 7.8 7.8 7.50 7.35 293 11.3% 24.7 2. 24.6 - 24.8 24.6 - 25.0 7.6 - 8.1 7.5 - 7.9 7.28 - 8.00 7.20 - 7.49 284 - 304 - -
".% 24.8 24ý8 7.8 7.7 7.49 7.35 280 24.7 - 24.9 24.6 - 25.0 7.6 - 8.1 7.5 - 7.9 7.27 - 8.00 7.20 - 7.50 273 - 289 24.8 24:8 7.9. 7.7 " 7.47 7.35. 255 24.7 - 24.9 24.5 - 25.1 7.6 - 8.2 7.4 - 7.9 7.25 - 8.01 7.21 - 7.51 248 - 261.
.24.9 24.8 . 7.9 7.7 7.45 7.35 222 24.7 - 25.0 24.6 - 24.9 7.7 - . 8.2 7.3 7.9 7.23 - 7.99 7.20 - 7.49 215 - 234 24.9 24.8 8.0 7.6 7.43 7.35 189 . 66 8 < 0.10
.00.0% 24.7 - 25.2 24.6 - 25.0 7.7 - 8.2 7.2 - 7.9 7.20 - 7.97 7.22 - 7.49 185 - 192 66. - 66 78 - 86 <0.10 - <0.10 Intake 24.9 . 24.8 . . 8.0 7.8 7.40 7.35 190 65 81 < 0.10 24.6 - 25.1 24.7 - 25.0 7.7 - 8.2 7.6 -. 8.1 7.17 - 7.95 7.21 - 7.50 184 - 192 64 - '67 80 - 84 <0.10 <0.10 Overall temperature CC)
Average Minimum Maximum Pimnephalespromelos 24.6 24.1 25.1 Cerioc/aphniadubia 24.8 24.5 25.2 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN):, UV-Treated Outfall 101, November 28 - December 5, 2006. Test Sample ID Temperature (OC) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Initial Final Initial Final Initial Final (ftmhos/cm) 24.8 24.5 7.9 7.5 7.45 7.18 300 Control 24.6 - 24.9 24.2 - 24.9 7.7 - 8.1 .7.0 - 7.8 7.25 - 7.91 7.11 - 7.31 294 - 311 24.8 24.4 7.9 7.4 7.45 -7.17, 294 11.3% 24.7 25.0 .24.1 - 24.8 7.7 8.1 6.8 - 7.9 7.25 7.91 7.08 - 7.28 288 305 24.9 24.5 7.9 7.4 7.45 7.17 280
*2216%
24.8 - 24.9 24.2 - 24.8 7.7 - 8.1 6.9 - 7.7 7.25 - 7.90 7.07 - 7.28 275 - 290 24.9 24.5 7.9 7.3 7.44 7.15 257 45.2%/ 24.8 - 25.0 24.2 - 24.9: 7.8 - 8.2 6.8 - 7.6 7.24 - 7.90 7.05 - 7.25 253 - 263 72.6% 24.9 - 24.5 7.9 7.3 7.42 7.16 223 _ 24.8 - 25.1 24.2 - 24.9 7.8 - 8.1 6.7 7.8 7.20 - 7.90 7.06 - 7.29 -218 - 230 25.0 24.5 7.9 7.2 7.41 7.17 .192
.. 100.0% 24.8 - 25.1 24.2 - 24.9 .7.6 - 8.1 6.6 - 7.6 7.19. - 7.92 .7.06 - 7.29 190 - 194 24.9 24.4 7.9 7.3 7.37 7.15 188 In2ake j 2.4.8 - 25.0 124.3 - 24.7 . 7.6 - 8.1 6.7 .- 7.6 .7.16 - 7.91 6.96 - 7.25 185 - 195 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.7 24.1 25.1 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected November 26-December 1, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant differences from the control during this study period.. Growth in minnows exposed to intake samples was significantly lower than minnow growth in the control.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in .intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. 11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Phinephalespromelas Tests were conducted according .o EPA-821-R-02-013 using four replicates, each containingten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylenie cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None.
- 2. Ceriodaphniadubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants,.Buffers, etc.: All chemicals were certified products used before. expiration dates (where- applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by EPA Method 170.1.
- 4. Dissolved oxygen was measured by EPA Method 360.1.
- 5. The pH was measured by EPA Method 150.1.
- 6. Conductancewas measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
- 9. Total residual chlorine was measured by ORION 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test Organism handling.during test, and maintaining .appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-0131 Any known deviations were noted during.the study and are reported herein. REFERENCE TOXICANT TESTS. (See Appendix D for control chart information) I. Test Type: 7-day chronic tests with results expressed as IC 25 values in g/L KC] or NaCI.
- 2. StandardToxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.
Sodium Chloride (NaC.I crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Useed: Moderately hard synthetic water.
4.- Statistics: ToxCalc software Versidn 5.0 was used for statistical analyses. 13
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA-600-4-79-020 (March 1983).
- 4. Quality AssuranceProgram: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nuclear Plant Biomonitoring November. 28 - December 5, 2006 Appendix B Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically. Induced Corrosion and Mollusks, During Toxicity Test Sampling
Table B- 1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of
-Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- December 1, 2006 Date Towerbrom PCL-:22 PCL-401 Cuprostat-mg/L ingfL .9 mg/L PF TRC Pbospha:e Copolymer mg/L Azole 03/12/1998 03/13/1998 03/14/1998 03/15/1998 03/16/1998 03/17/1998 03/18/1998 09/08/1998 01014 0.005 09/09/1998 0.031 0.011 09/10/1998 0.060 0.0021 09/11/1998 07055 0.019 09/12/1998 M.044 0.015 09/13/1998 0,044 0.015 09/14/1998 0.t14 . 0.015 02/22/1999 02/23/1999 02/24/1999 02/25/1999 02/26/1999 02/27/1999 02/28/1999 08/18/1999 0.015 0,069 0.024 08/19/1999 0.012 0.ý0 68 . 0.024 08/20/1999 0.023 0.0f) 0.024 0.120 08/21/1999 .0.022 0 06* 0.0024 08/22/1999 0.022 0 .06S 0 024.
08/23/1999 0.025 .i)06S 0.024 08/24/1999 0.016 067)C7- 0.023 I
Table B- I (continued). Sequoyah.Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date Towerbrom iiiiPCL-222i PCL-401 C-L-363 Cuprostat- 1~Vi3U mg/L mg/LI mg/L m-fL. PF Lfl!/L.. TRC ?Ph4a**if* Copolymer DMAD mg/L (Žu4 0.0,,~ ~ ~ ~~~... ..........
~~*}iiii~a ai... ...
ll o 0 8 ....................... .Azole 01/31/2000 < 0,002 002 6*:'ý;---ý 0.009 . -. - 02/01/2000 0011 0,02: 0028 - 02/02/2000 0.028 0 .O60009 02/03/2000 0.008 0*02i7 0.009 - 02/04/2000 <-0 .006 0,iii!*!i3 i iiiiii'* 0.00 ............... 02/05/2000 02/06/2000 0.009 i0.07 0.009 0.0059 07/26/2000 <0 00,88
<0.002 07 0,0535 0*
00.19 001 ,*. 0090 . .. . . .... 07/27/2000 07/28/2000 07/29/2000 <0.0076 0.055 , 0:019 07/30/2000 < 0.0152 0ý055 0.019 07/31/2000
- 0.0814 1' : 053 0.019 : :
08/01/2000 0.0076 0 215 0.019 12/11/2000 <00192 0** *i 0.020 06 12/12/2000 o0o124 0.**-5 0.020. 0 12/13/2000 12/14/2000 < 0.0087 01025 0020 - 12/15/2000 0.0120 O0.05 0.020 0,00 12/16/2000 <0.0036 (10-21 0.020 12/17/2000 <0.0036 0.02 020 iii~iiiii* iiiii~ii
- s' 08/26/2001 0017 0.06 0.021 0006.
08/27/2001 <0,0096 t).06 0.021 00 OS/28/2001 <0.0085 0.06 0.021 0*1 =. 08/29/2001 <0.0094 059 0.020 08/30/2001 <0.0123. 0.06 0.021 0 08/31/2001 <0.005 0.059 0.020 11/25/2001 <00044 .- 11/26/2001 <0.01 19 0,024 0.02 -01 05 11/27/2001 0.0137 0ý023 0.019 0.7 11/28/2001 <0.0089 0,022 0.019 0.*006 11/29/2001 0.0132 0 0 214 0.02 . 11/30/2001 < 0.0043 0*0ý4 0.02 12/09/2001 <0.0042 12/10/2001 <0 .0042 12/11/2001 <0. 0104 12/12/2001 -0.0128 0,024 0.02 v 12/13/2001 <0.0088 0l,02 4 0.02 12/14/2001 0.0134 0 024 0.02 o.7 2
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling,. March 12, 1998 - December 1, 2006 Date dM Towerbrom PCL-401 C Cuprostat- 1140,M mg/I Hyp~dikiite . mgL;f mg/L PF mglL. TRC Phisii t& Copolymer DMA mg/L TC..... Azole 01/02/2002 < 0.0079 20.02 G 01/03/2002 <0.0042 0.... 0.014 01/04/2002 ~ 0.0124 40.014 00 02/27/2002.......... < 0.0042 .... 0.023 01/06/2002 < 0.0042 0.024. , 0008 01/07/2002 < 0.0089
- .002 0.014 0/04/2002 < 0.004 ......
0*/*5/2002 < 0.0048 i 0.023 - 08/06/2602 0.0143 )2,t:3 0.0230 18 08/07/2002 <0 0041 I J05 0.01900.23. . 0/08/2002 < 0.0041 0.055 0.008 : 10/06/2002 05/10/2002 < 0.0041 97 G"4': . 0.0:19:*ii~i!i~iii o.oo8 105/0/2002 00153 . . 0.018 000-05/06/2002 0.0092 0.018 08/07/2002 a0.0127 0.018 M1 05/08/2002 0006134... 0.019 01/09/2002 <.0.05 0.018 01/10/2002 < 0.01S 0.019 0.009. 01/12/2003 01/14/2002 <0.0035
<0.0018 0.526 iii~{i(({ 0.020
-iiiii}iiii . i :i{:*~ ~ ll((i iii:*iiiiii~i:
01/15/2003 <0.0063 I 0
.02$* 0.012 0+025 01/16/2003 <0.0093 02 0.020 01/17/2003 <0.0037 .026 0.0109 007 04/06/2003 <0+0073 0.1 04/07/2003 <0.0159 3 0.021 0 04/08/2003 <0.0117 OS 0.021 04/09/2003 <0.0139 0.021 G 04/10/2003 <0.0113 0021 04/11/2003 <0.0073 0.0220 3
Table B-I (continued). Sequoyah Nuclear Plant-Diffi.ser (Outfall 101) Discharge Concentrations of Chemicals Used to Contr6l Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -December 1,2006 Date . Towerbrom PCL-401 Cuprostat-1{y~dknte mg/L mg/L PF TRC Copolymer mg/L Azole 06/15/2003 <0.0045 06/16/2003 <0.0037 0.020 06/17/2003 .......< 0.0048 0.014 06/18/2003 < 0.0048, 0.014 06/19/2003 < 0.0085 0.020 06/20/2003.< 0.0048 0.020 08/03/2003 <0 .0050 08/04/2003 <0.0050 0.020 08/05/2003 ..... <0 0051 .0.020 08/06/2003 <0 0084 0.020 08/07/2003 0.0129 .0.020 08/08/2003 0.0153 0.020 10/05/2003 <.0043
<0. 0.020 10/06/2003 <0.0043 0.020 10/07/2003 <0.0090 0.020 10/08/2003 <0.0106- 0.020 10/09/2003 0.0181 0.022 10/10/2003 0.0183 0.024 02/01/2004 0.0093 0.009 02/02/2004 <0. 0034 0.009 02/03/2004 <0.0034 0.009 02/04/2004 0.0124 0.009 02/05/2004 <0.0034 0.009 02/06/2004 .... 0.0105 0.009 05/04/2004 - <00123 0.019 05/05/2004 <0.0144 0.014 05/06/2004 <0.0146 0.013 05/07/2004 0.0227 0.020 05/08/2004 0.016 0.021 05/09/2004 <0.0104 0.020 07/04/2004 0.0217 0.019 07/05/2004 <0.0085 -0020 07/06/2004 -iiiý <0.0077 0.020 07/07/2004 0.0252 0.019 07/08/2004 00.0223 0.019-07/09/2004 :: 0.0182 0.020 4
Table B-1 (continued). Sequoyah Nuclear.Plant Diffutser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date Towerbrom PGLi222 PCL-401 Cuprostat-PF Nalco mg/L {~~iiiW:~ii!iii mg/L mg/L 73551 TRC hoPhLteK Copolymer Azole mg/L EO/PO 11/07/2004 <o.o187 o014 0.000 11/08/2004 <00.192 0047 0.030 11/09/2004 <0.0233 i k4S .i-0.016 11/10/2004 <0.0149 fo4ý7* 00.16 11/11/2004 <0.0149 04i)"9- 0,017 11/12/2004 <0.0253 0.iZ'b A4S 00.17 02/06/2005 <0.0042 f:,03 0.010 02/07/2005 <0.01 16 02 7 0.010 0.007 02/08/2005 <0,0080 0.02i 0010 0.0199 . 0.010 02/09/2005 02/10/2005 <0.0042 0.010 02/11/2005 0.0155 OG2 0.010 0.007 06/05/2005 0 .0063 . 06/06/2005 0.0043 06/07/2005 0.0103 06/08/2005 0.0295 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0fý 0.009 07/18/2005 0.0150 0,(26~ 0.009 07/19/2005 0.0163 ý)'02-6 0.009 07/20/2005 0.0209 01t) i6. 0.009 0.014 07/21/2005 0.0242. 0Ai . 0.009 07/22/2005 0.0238 OA154 0.018 0.014 10/30/2005 0.0068 - . . - 10/31/2005 0.0112 11/01/2005 0.0 104 11/02/2005 0.0104 11/03/2005 0.0117 11/04/2005 0,0 165 . ... 111/14/2005 0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 0.'023 1 11/-I8/2005 0.0200 11/19/2005 0.0116 5
Concentrations Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfali 101) Discharge Of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, F March 12, 1998 - December 1, 2006 CLC.!63 Cuprostat-PF H1O Date Towerbrom g/{i*{l mg/L . g/L mg/L y.........
... TRC PCL-222ii~ iiii((i~{ D{6AIY Azole A
11/12/2006 0:0055T .. 11/13/2006 0.0068 : .. ........ 11/14/2006 0.0143 11/15/2006 0.0068 11/16/2006 0.0267 . . 11/17/2006 [ * ..... 0.02-22 11/26/2006 0.0188 11/27/2006 0.0138 11/28/2006 0.0120 11/29/2006 0.0288 11/30/2006 0.0376 12/01/2006 0.0187 6.
Sequoyah Nuclear Plant Biomonitoring November 28 - December 5, 2006 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I I I Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx , Bus rUPS Client. P.O. Number: N/A Asheville, NC Other (specify): Express Courier 28801 'General Comments: Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Phone: 828-3 50-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Chevy Williams 11/27/06 Express.. 2 urier - 11/27/06 Express Courier 11/27/06 ETS 11/27/06
./' ,2_g,- . _ _____,___
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 61C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples wvith .amole time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked forSaturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of -I Client: TVA :-_Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 3 5 1 Depot Street. FedEx UIPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier 28801 General Comments: . Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn. Fax: 828-350-9368 Field Identification/ Grab/Comp." Collection Date/Time Container Flow p. i 4-4 .Ol.: .. Sample Description Number & MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) ___________ Collected .__ _._*_'_: _':____::._.:_... .. . ;__,_-_* Date Time Ye fYs o Tae ETS Log Arrival Temp- By Time .Ap
-". -,_,_,,...____.__,-_.:Inches Number',. . _°C, ".anc(
SQN-101-TOX Comp 11/28/06-11/29/06 0826- 2 (2.5gal) NA ""'- 0 72 6 0 .. .. . . SQN-INT-TOX Comp 11/28/06-11/29/06 0843- 1.(2.5 gal) NA _ _ _ _ _ _Sample Custody Ftll In From Top Down "*L C a- -
.Relinquished By (Signature): Date/Time Received By (Signature): Date/Time C evy - 11/29/06 Express Counier 11/29/06 Express Courier 11/29/06 ETS 11/29/06 cm' IL)-C .
Instructions: Clients should fi'll in all areas except those in the "'laboratory Use" block. Biomonitoring samples are preservcd by storing them at 6°C and shipping them .in ice. The hold time for sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with amnple time to initiate testing within that time frame. Sar shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page -I- of -I-V V Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier 28801 General Comments: Facility Sampled: S'equoyah NP NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368
- . __ 1 __ __ __
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biom0noitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time foi sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the lahoratory will receive the samples with ample time t6 initiate testing within that time frame. Sa shipped overnight on Friday via FedEx or UPS must be marked for Saturday, delivery or they will not arrive until the following Monday.
nvironmental Testing Solutions, Inc. F~ace I ot b Environmental Testing Solutions. Inc. Page 1 orb Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0), Species: Pimephalespromelas Client: TVA Countv: Hamilton Facility: Sequoyah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: 2- p,,, Dilution preparationinformation: Comments: Dilution prep (%) 11.3 22.6 452 72.6 100 Effluent volume (mL) 282.5 . , 1130 1815 2500 Diluent volume (mnL) 2217.5 2*1l 1370 685 0 Total volume (mL) 2500 '2"500 2500 2500 2500 Test organism information. Test information:. Organism age: 2A -i -Lk 00's'ý. ozF- Randomizing template: 1 4eAuo0 Date and times organisms vi-Ot 7\- k6co -M %(Coo Incubator number: were born between: .1 C. Organism source: Ae- 9-c* e' t'-"Yi-O Artemia lot number: 6- 12.0q C Transfer bowl information: pH= Temperature = C Total drying time: Lk_-___ s_____
-__,_____,____._ _"z.. "2.. Date / Time in: -
- o Average transfer volume: Date Time out: ai-o0-o:,
Oven /temperature: to-LI (O '-C. Daily feeding and renewal information: Day Date Morning Afternoon: Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 iiSs-& 9 1.i. i'yUA CA-$ 9'- 1177.t 40 I2,Re-01o 0420 I.:S I'S"- 1 1\ 21,:-, k - , 2-., 0 140.7-3__,____ ngk I 3'D1 _____ s1017. 0--ot.t 0- t1 2.IV ,9-" 0. k>* 6___ la.DV..Ot q, lSJ~t. [$15 Z- JZ-*-C*aB 6o ZOt.0\ e*O"L. 4 I 6C)01..... .ZGL-0CA* 0612ot.Ot O*- Control information: Acceptance criteria Summary of test endpoints:
%Mortality: . 0", *20% 7-dao LCso 1067'o Average weight per initial larvae: .705 NOEC. < it ---17.,
Average weight per surviving larvae: 0.1OS : 0.25 mg/larvae LOEC 4 I, 31o ChV -' i -l"*?,o ICh i 07 I.X'e'aurre-t, buý-.P- vzýpokys'e- 41POTAP-slý'
-F;tsT rzesoL:Y-s, Po-e jýmp-,,AzLe.
trivircinmentai i esTing ,ýciiuuons, Inc. Fvae -1 or 0 tnvironmentai 1 estln2 ~OIuHOnS. Inc. l-~age .~ or o
.Date: }'6&
Species: Pirnephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 11.3% 22.6% L A I B C D E F G H -1 J K L 0 1 /0 /0 //0 10 I 10 /0 / /o 1 /0 /10 ( 20 /O /00/0 1t3D IQ (7 10
/ /0 10 3 (0 /0 /0 /0 /b /0 10 /0 /0 .1t0 '0I 4I /o /0 /0 1 /0 1 0 /0 t o, /C
//0 0// /0 1 /to C/0 /0 /O '6 6 /0 /0 /0 /0 /0
- t. /
t: / /c . 0 to O /0 /I /1 A Pan weight (mg) Color identification:b.&-VJJ Nq 1 IY.3l 13.50X-Vc 1Y7.q IN3' , jq H 1 rlq.qIq1.iA IIN,.q Analyst:, U'41B* B = Pan+ Larvae weight " Analyst: (mg).=A ] Larvae weight (mag) A-A B.
" _ ",0"1."1..2 (o~ 'q I ,.ql, " k*. Z Io *-6 *A,' .R I.-So (0 ,t,'I. .c9,...
-Weight per initial number 'N of larvae (mg) "P e4;v 0V A" C'. z,.. Nb
- c. NO
'.0 \0'
= C / Initial number of larvae ND *'.
KIN 'a 0' UO 0' 0~ 0 Average Percent weight per reduction initial from cbntrol 0.10 S 9,.007 o' t'O 1 number of (%) larvae rni ) " I I ! I Comment codes: c = clear, d = dead, fg = fungus, k = killed; m = missing, sk sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w wounded. Cd Calculations and data reviewe~d: - Comments: " PO, e*C GZ,*rA * *T ie te o*-Tie. , nAtu 7Cýk siL AP-x-A I(rti 1,3 S
Environmental Testin2 Solutions, Inc. I -:t;' , -~ .a Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: / e'ao,- Survival and Growth Data Day 452/o 72.6% 100% M N 0 P Q R S T U V W X 0 / C) 1o 0 I10 1o 10 [ to IO 10 b IG 11 ( I
'10 1D I C0 0 (0 /0 Ito 1. I)0 /0 to 10 1 /0 /0 /c 10 /0 i /0 70 /0 /0
/o
.10 /D O 0 O to /0 10 ( 10 0 10 /o
,'0 ID
. 10 0 10 I0 /O /D to /0 t0 /o 6 /0 JO3 /0 'i /0 /0 /0 /,0 I/ /0 /O /Q 7 . /i.o o /0 t'O ;O /o /c> ic>O /*t A = Pan weight (mg)
Color identification: blUO+S.- 13.5,8 PI.'-S1 R.0 H-.59 )'.85 13.-. qt4, lS.4 I.q)q 13V4 !S.;(O '."-0 Analyst: i,. IB B = Pan + Larvae weight (mng) ,ao.'o a1.qo90~ ai-.iD c.o*bs iO.*S ao-whiiqria ao.40 -ilz ao .99 QJI b Analyst: ._1.-a Tat'5 Larvae weight (mg) A- B Weight per initial number O , oflarvae(mg) > ,, A o '. 4 C /Initial number of larvae ,.P -ID "~ ',.o .
"" , 0' 0' c"' ' "
Average Percent w eig ht p er red u ctio n .... initial from control Oo0'.a,0oe ,Sc 2 number of (0) larvae (mg) Comment codes: c = clear, d dead; fg fungus, k killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w= wounded. o
'"Calculations and data reviewed: 4 Co mm ents:
Environmental Testing Solutions. Inc. . _F1_ I - ý - Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 1/" . 0(0. Survival and Growth Data Day 100% Intake Y Z ] AA BB 0 /,io c, 0 (C) /0 / /0 2
/ .I 00 0 /0 3 1I (0 (0 10 1.
4i0 10 10 /0
/0 /0 /0 /0 6 6((5 -(5 'C /o Q
C) /0 10 /D A Pan weight (mg) Color identificationbl:LkyO-t N-.30 J.q.45 l.J5 )lo.0/ Analyst: ..___ ____ B = Pan + Larvae weight (mg) .O.qq o.38W.30 )2j.3£ Analyst: _ ..... _l_______a _ Larvae weight (mg) =A - B A,.*Js.3 t. U.-11 Weight per initial number of larvae (mg) , A C/Initial number of larVae C \9 \. ", Average Percent weight per reduction j-2. initial from control 0 . number of (%) larvae (mg) Comment codes: c clear, d =dead, fg fungus., k killed, m= missing, sk sick, sm unusually small, Ig unusually large, d&r decanted and returned, w =,wounded. Calculations and data reviewed: Comments:
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimnetph ales pront elas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2906 Reveisced by: / LV,_+Q .ý Not for Corripliatce Asses~sineot,,internal Laboratory QC ' C.o* r rqtHon(%/*) 1R.t kto ,t5d.rd. m*e*r.. t A- PP-n r-(L- (-rI Bhi Lo' LI'. (nSAC) WCchia 5rhl wet h (.e c tM- .ttut nr C hlt Oýtdr~r hIfr ghtt Irtlti rruerb ro
%dn tl..nrur.'oi 1n ,tthr/ 1,4bvfM Co,.it i t-I tof
-6r.t0 rdretoe I iareo. .cJ'h'lec) . A-B' nrmb.rotlr'. (e ) ro.li nr) (I..tO r" aroerOr,, l en) (%) - ,r.e-rnrtl.r' . (M]- contrl (V/.)
A 10 10 14 29 21.36 7.07 0.707 0.707 Contr'ol B 10 10 14.31 21.57 7.26 0 726 0.705 2.6 0726 100.0 0.705 2.6 Not applicabil C, 10' 10 15.32 22.38 7 06 0.706 0706 D 10 10 13.59 20.41 6 82 0 682 0.692 E . 10 10. 1350 19 94 6.44 0.644 0644 11.3% F 10 10 14.72 21.13 6.41 &0641 0.6!12 5.2 0.641 100.0 0.642 5.2 9.0 G 10 10 14.34 21.16 6.82 0.682 0682 14 10 10 14.12 20.13 6.01 ,0.601 0.601 1 10 10 14,84 . 21.52 6.68 0.668 0.666 22.6/ J 10 10 14.97 21.47 6.50 0.650 0.66"9 2.6 0.1650 . ' 100.0 0.669 2.6 5.1 K 10 10 14 14 2081 6.67 0,667 , 0667 L 10 10 - 14.19 21.11 6.92 0.692 0,692 M 10 10 13.58 20.07 6.49 0.649. ... 0.649 45.2% N 10 10 14.45 21.90 7.45 0.745 00677 7.1 0.745 100.0 . 0.677 7.1 4-.0 o 10 , 10 14.04 20.4 . 6.33 0.637 0.637 P to 10 14.59 21.36 6.77 0.677 0.677 Q 10 10 14.85 20.05 6 00. 0.600 0.600 72.6o/ R 10 10 13 64 19.02 6 18 0.610 0.609 2.9 0.619 100.0 0.609 2.9 13.7 S 10 10 14.49 20.77 6.28 0628 0 628 T 10 10 13.84 19.32 5.68 0.588 0 586 U 10 9 15.04 20.40 5.36 0.596 0.536 100% V 10 10 13.81 19.76 " 5.95 0.595 0.607 6.8 059$ 97.5 0.592 913 16.1 W to ' 10 15.26 20.97 5.71 0.571 0.571 X 10 10 14.70 .21.36 6.66 0.666 0.666 : Y 10 10- 1430. 2044 6.14 .0614 0.614" 100% Intake z 10 10 1445 20.38 5.93 0.593 0615 2.9 0 593 100.0 0.615 2.9 . 12.8 AA l10 10 14.15 20.30 6.15 0.615 0.615 BB 10 10 1601 22.38 6.37 0,637 0637 OutlfAll 101: MSD=- Muiroam Stpiificant Difference Dunnett': MSD value: 0.0590 PMSD = Percent Minrrom Sitific'anl Difference PMSD: 0.5 PMSD isa3measure of test precision. The PMSD is the ntirrssmperccttt difference btweten the control ansdtreatment that, can be declared statistically significant in a whole effluent toxicity I Ott a'erge, a significantdifference occurs for Envs-oncnenta[ Testing Solutions, Inc. ichronic toxicity tests whets a toxicatt reduces Pirecphales g-owvh.by 15.5% ftoro the control (detertined Intake: through referetsce toxicant testtng). Dunnett's MSD valtie: 0.0247 Lower PMSD bound determined by USEPA(10th percentile) 9.4%. PMSD: 3.5 - Upper PMSDbound detetiTrned by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPAusing 205 tests condtlucted from 19 laboratories for Pimepholes growth it chronic reference toxicantt tests USEPA. 2000. UJnderstantding and Accounting for Method Variability in Whole Emfluent Toxicity Applications Under tlheNational Pollutant Discharge Elictnation Programt EPA-833-R-00-003. US Envirdnmental Protection Agency, Cincittati, OH.
Non-treated November 28 - December OS, 2006 Statistical Analyses LarvaI Fish Growth and SorvivaI Ttt-7 Day Growth Start Date: 11/28/2006 Test ID:" PpPRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Outfall -01 End Date: 12/5/2006 Lab ID: ETS'Ewvir. Testing Sol Sample Type DMR.-Discharge Monitoring Report Sample Date: Protocol FWCHR-EPA-821-R-02-013 Test Species: PP-Pilnephales ptometas Comments: Non-treated Conce% 1 2 3 4 D-Contiol 0.7070 0.7260 0.7060 0.6820 11.3, 0.64.40 0.6410 0,6820 0.6010 22.6 0.6680 0.6500 0 6670 0.6920 45.2 0.6490 07450 0.6370 0.6770 72.6 0.6000 06190 0.6280 0.5880 100 0.5360 05950 05710 06660 Trnaform: Untransformed 1-Tailed Isotonic Conc-% NIran N-M-an Mean Mlin MaN CV% IN t-Stat Critical MSD Mean N-Mean D-Conttol D 0.7053 1.0000 0.7053 0.6820 0,7260 2.556 4 07053 L.000 "11.3 0.6420 0.9103. 0.6420 0.6010 0.6820 2.155 4 2,54S 2.410 0.0598 06628 0.9397 22.6 06693 0.9490 0,6693 0.6500 0.6920 2580 4 14)50 2.410 00598 0.6628 09397 45.2 0.6770 0.9599 0.6770 0.6370 07450 7.139 4 1 138 2410 0.0598 0.6628 0.9397 "72.6 0.6035 0.8626 0.6085 0.5880 0.6280 2.944 4 3.897 2.410 0.0598 0.6085 0.8628 "100 0.5920 0.8394. 05920 0.5360 0.6660 9.284 4 4.562 2.410 00598 0.5920 0.6394 Auxiliary Teots Statistic Critical Skew KCrt Shapiro-Will's Test indicates normal distribution (p > 0.01) 0.942544222 0.884 0.702791759 0.883 183654 Bartlett'sTest indicates equal vairances (p ý 0 21) 7.118869305 15,08627224 Hypotshesis Teat (I-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp N1SB6 NISE F-Prob df Dtiruett's Test <1.13 11.3 0059831485 0.08483727 0.0074371 0.001232694 .0.0018978 5, 18 Tiauonsots us D-Conorol Linrrar'lotcrpolatian (20 Resmopln) Point % SD 951/6 CL(Eip) Skew IC05" 9.376 16.565 4.511 76.887 1.2631 ICID 59.,35 ICI5 877608 1.0 IC20 .>100 0.9 IC25 >100 IC40 >100 08o IC50 >100 07 indicates IC eslimate less uxt the lowest concentr-ation n0.6 00 0.3 0,2 0.0 0 20 40 60 00 100 120 Dose % Do~s -Rspon.se Plot 0.0 07 .1 -tall, 0.06 level
.. .. . .. ... . ...t.....fl o
o 0 sqn_lII-28-06duta
Non-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11128/2006 Test ID: PpFRCF. Sample. ID. TA I Sequoyah N-clear Plnt, Intake Erd Date: 12/512006 Lab ID: ETS-Envie. Testing Sol Sample Type: DMR.-Discharge Monrtoring Report Sample Date. Protocol: FWCHR-EPA-821-R-02-013 Test Species PP-Pimephales ptomelas Comments: Non-Deated
.Conc-% 1 2 3 4 D-Control 0.7070 07260 0.7060 06820 100 0.6140 05930 0.6150 0.6370 Trnsnform: Untranslormed 1-Tailed Isotonic Cone-% Mean N-Mean IOe-an Min Max CV. N I-Slt Critical MSD Mr-sa N-Mean D-Cotntol 0.7053 .1.0000 07053 0.0S20 .07260 2.556 4 0,3053 1.0000 "100 0.6148 0.8717 0.6148 05930 06370 2.923 4 7111 1.943 0.0247 06146. 0.8717 AuxiliaryTeasts Statistic Critical Skew Kurl Shapuo-Wilks Test indicates normal distrtbutiotti (p> 0.01) 0.883914053 0.749 . -0.1195042 -0.69030905 F-Teit indicates equal voiarnces (t = 1 00) 1,006193519 47.46722794 Hypotheis Test (-ltail, 0.05) MISDu MSDP NSB MSE F-Prob df Homoscedasitc t Test indicates significant dtffetences 0024729467 0.035064824 0,0163805 0000323917 3.00-04 1.6 Tteat*mnets vs D.Control Linear Interpolation (200 Reampltes)
Point % SD 95% CL(E.sp) Skea, - IC05" 38.964 5.006 24.112 63.702 0.8889 ICI0 77926 IC15 >100 10 IC20 >100 0.9 IC25 >100 IC 40 > 1006 O , IC50 > 100 0.7 nitdicates IC estimate less than the lowest concentsation 0.6 c" 005 0.4 0., 0.2 0.1 0.0 , 0 20 40 60 80 100 120 Dose %. Des e-Rweip.nse ]lot 0.. 0.7 .1. 005 level
-tail.
of sigNificance 0.6 0. tO90.4 0,2 0.1 0 0~ 0
.sq,_l O6daoa
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelis Daily Chemical Analyses Project number: 2906 Reviewed by: Concentration Parameter .Da 1)a 1 Day 2 Day -3 D -- 4 Day 5 Day 6 Initial Finat Initial Final Initial Final Initialt Finalt Initial Final Initial Final Initial Fina _PH(SU) 7.69 7.35 7.75 7.03 7.59 7.17 7.821 7.19 7.89 7.22 7.90 7.04 7.657 DO (mg/L) 7.7 7.7 71.8 7.1 7.7 7.4 7.6 7.4 7.7 7.3 7.8 7.1 7.8 Conductivity (pmhos/cm) 318 318 315 307 3 300 309 Control Alkalinity (mg/L CaCO3) 58 59 58 Hardness (mg/L CaCO 3) 92 86 90 Temperature (°C) 24.6 24.5 . . 24.5 24.'6 24.7 24.8 24.8 24.5 24.7 24.6 24.8 24.2 24.6 pH (SU) 8.00 7.31 7.43 6.92 7.28 7.11 .7.33 7.13 7.44 7.13 7.66 7.02 7.39 1) S (mg/L) '" 7.7 7.7 7.8 6,9 7.6 7.3 7.8 7.2 7.8 7.3 8.1 7.0 8.1 Conductivity (lsmhos/cm) 301 ,304 295 286 . 289 284 290 Temperat~re (°C) 24.8 .24.2 24.7 24.5 24.8 24.8 24.8 24.5 24.7 244. 24.8 24.5 24..7 pH:1(SU) 8.00 7.26 7.42 6.93 7.27 7.12 7.32 7.14 7.43' 7.14 - 7.64 7.02 7.38 6.7 7.6 7.2 7.8 7.2 7.8 7.1 8.1 7.0 8.1 22.60 DO (mg/L) .7.71 7.5 7.8 . Conductivity (jimhos/cm) ., 287 289 282 275 275M273 277 _Temperature (°C) . 24.8 24.2 24.7 24.5 24.8 . 24.6 24.8 24.4 24.7 24.4 24.9 24,3 . 24.7, piT (SU) 8.01* 7.27. . 7.40 6.89 7.25 7.14 7.29 7.15 7.43 7.14 7.58 7.07 7.36 " _ 2DO (mg/L) 7.7 7.5 7.8. 6.4 7.6 7.4 .7.9 7.2 7.8 7.0 82 6.9 8.1 4S.2% Conductivity (mlmhos/cm) 260 261 256 260 248 251 252 Temperature (°C) 24.9 24.2 24.7 . 24.7 24.9 24.9 24.9 24.6 24.7 24.3 24.9 24.6 24.7 Lm-(S U) 7,99 7.27 7.39 6.88 7.23 7.13 7.27 . 7.16 7.38 7.18 7.56 7.P07 7.35 7 2.6 % DO (mgfL) 7.7 7.6 7.7 6.3 7.8 7.4 8.0 .7.3 7.8 7.0 8.2 7.0 81 Conductivity (Otmlios/cm) 234 225 . 222 218 215 218 222 Temperature (°C) 25:0 24.31 24.8 24.6 24.9 24.9 24.9 24.4 24.8 24.6 24.9 24.2 24.7 1p-H(SU) .7.971 7.291 7.40 6.89 7.20 7.15 7.221 7.16 7.3,S 7.18 7,49 7.17 7.321 DO (ing/L) . 7.71 7.61 . 7.7 .27.91 7.41 8.21 7..2 8.3 .0 8.2 7. . .8.1 Conductivity Qtnlios/cn) 189 1.90 189 1 1100% Alkalinity (mg/L CnCO 3) 66 66 66 Hardness (mg/L CuCO 3) 78 8 80 Total-Residual Chlorine (mg/L) <0.10 <0. <0.10 Temperature (C) " 25.1 24.6 24.9 2 25.0 25.0 24.9 24. 24.8 24.61 24.91 24.1 24.7 pH (SU) . 7.95 7.301 7.39 6.91 7. 17 . 7.16 7.20 7.19 7.32 7.11 7.44 7.08 7.32 DO (mgfL) . 7.71 . 7.71. 7.9 6.6 7.91 7.51 8.21 . 7.5 8.01 7.11 8.21 7.4 8.2 Conductivity (_tmhos/cm) " 191
- 192 191 184-189 188 . 192 11100/. Intake Alkalinity (mgy/L CaCO 3) 65 64
.Hardness (mg/L CaCO3) 80 84 80 Total Residual Chlorine-(mg/L) <0. 10 <.1 <0. 10
,Temperature (C) 25.0 24.4 24.7 24.6 25.01 24.9 24.8 244 24.8 24.4 24.9 24.31 24.7
t~nvironmeniai i esting, 3oiut ivw, 111L. Species: Pimephalespromelas Date: I,1q 0.ob Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistry:
- Day
~~~~~~Analyst J"-r- . # *! *,te*-
( .mhos/crn) .* s 3 3i1* n (rt CaCOr/L) g "5q l- a rd n ess " -- M (DO CaC0 3/L) Tern erature,,C. i'-., .WS -iA C. 24,1 . z4 pH(S.U.) *.00 "* .3j .* q*,, _, pq Q.
'.2.s -
Alalndciity Conductivity 30. . Ternserature. 0 C iA q~2' . 2 '..S "'A.*a 9r rnhos.crn) ,/.* .36 72.6% Dm aOrncL) - (pmhos/cmn) 30* Act"L
.( *mhos/cm) *LO~ *qAw -. 5 i
.Tern erature (0 C)
Conductivity "'-IA"& ' *L AZt
" ._ LA.-..
*v4.-1.-t. iM"A l
.. Hp(S.U.)
Temperature('C) 9.o5-v. 3.2,' I 3~q H 4- l-'- 1%4"s *.L-2)i
-LA - a R 72.6/
.6DO (m
.4o.% DOg.!mPL)
Conductivity 9* 6_1'_ Conductivity ,/7 Temperature (-C) aS- Z-'4. -
- v. I VA so
"-z, (prmhoslcm) 2.6% TeO(ran pr atr/L) (aCO/
CaCO )" -L 9l -I Z IP V4 , 3 Conducti vityv _(m Fnhos, cq () .L) 2. z
- Alkalinity * ,
(mag CaCO31L) U0(I:5.,"
-' Hardness*
Hardness *g (mag CaCO,/L)
*.TR chlorine~TR chlorine"".'"0'I"C)
(rag/L) ".r ~ *,.,.'Mmc ,t
° Temperature ('CQ Ternperature (0 C) IS-.0 "S, \ -i,q. ...
- Z /.l,(' . -L~S, 0"* -LSzz ,* I 10'ý D m/- 4 inae Initial I Final In al a, I Final I Initial i n Final
Environmental Testing Solutions, tnc. I Species: Pimeph alespromelas Date: I'2&~ Client: TVA / Sequoyah Nuclear Plant - Non-treated I3 Analyst 11. 1t k-IDay 1 jj [qý I Concen- Parameter . trati Un CO N T R O L pH ) , __ ) .-------- -_q_ _ DO (rnL (5 Conductivity (ýmhos/cm) Alkalinity (mg CaCO 3IL) Hardness . - (mg CaCOJ/L) Temrn erature ('C) 7,k R 2 I. s 7,A '1 7-1.. TA - "'A.Z_ z.4 ,Lq. p1I(S.U.) 7. 113.% DO(mg.L) Conductivity (4n-hoslcm) A( 4
*Temperature (°C) "Z -' v4. . S LA. .2-22.6% DO (mgiL) q e) 2.
,p I S.U.)
C ond u ctivity .. 9.-- 2 q.A5 " *q 3 _"4.- ,. C', q~s ' 2z- 130 zt. , Ternperature ('C) -Z. 7.4. MIA.
-- L~Z p6°(S.U.) _q 1 . -- 2 45.2% DO (mg1n IL)l) " 5 Conductivity
( rnhos/crný( y
- Tern, ,perature(0 C) i .t 7z('C) . 7.
pH (S.U.) .. . -c.b. 9 2.3 72.6% DO.(m g_L) " (a Conductivity "A Ter erature ('C) 141 TAIL! ?q, oLI -a4.3 .- m1. pH (S. U.) q 3 1
'Hardness (m/ g CaCO(L) R0 TR Chlorine (mg/L) .O Temperature ('C) 14q.A Zq.A ' 244. 1. 2q.. ..
H,(S. U.) .,Q 100% DO (mgL)_ 6.2 In ta k e." 0.2" . .. . i.0 Conductivity L ( rnhoslcrn)9 69e Alkalinity (mg CaCO 3IL) Hardness So (mg CaCOjfL) 1Ter TR chlorine (mg/L) erature(°C) f 0 E Zi,. zq.l ZZq.3 Initial Final Initial Final Initial Final Initial Final
Environmental Testinla Solutions, Inc. Page I of7. Environmental Testin~ Solutions, Inc. Page 1 of 7. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0). Species: Ceriodaphniadubic Client: TVA County: Hamilton Facility: Sequoyah Nuclear Plant Treatment: Non-treated NPDES 0: TN 0026450 Outfall: 101 Project fl: -7 Dilution preparationinformation: Comments: Dilution prep (%) 11.3 22.6 452 72.6 100 Effluent volume (mL) 282.5 ac 1130 1815 2500 Diluent volume (rnL) 2217.5 [' 2 - 1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: <-A.860,-Mt 0 .jI Randomizing template: c0,tAN.- Date and times organisms i\ - 'l-C, %-lie-ro iOt,, Incubator number and were born between: shelf location: Organism Source Cups: . , , YCT batch: Culture board: 21-2.- (8, Z ll,fl_ Transfer bowl information: pH = "i, Temperature =V-. 5 Selenastrum batch: 11- 5-0o,, Daily renewal information: Day Date Test iniciatijn, Control water Sample numbers Analyst renewal, or batch used used termination time 0 _2-o,., I .. V
\-O, A ofZ,% \C. \ -- ,
2 t\-1o-ot-. lo\- 06 0c 11 , 0 CA 4* 3 t-. . t, ' St -?.. u<IS a t,, %\ k . . -a l' ,4 ,.0 4 . .o0-S2 _ wz-z6- (. ok. Izo.-\* o - , 6 12-oq-ot1 o -0.1. 11,O1"* 4 L '-016-A,/ __7_ _____ 1 01$5q Control information: 1 2 Acceptance criteria Summar of test endpoints: % of Male Adults .- ol o - 20% 7-day LC50 > fOOTrý % Adults having 3r" Broods:. /o007 /oO107 80% NOEC /o_7_ _ % Mortality: 0 7ý 07. *20% LOEC >too 1. Mean Offspring/Female: Z I . , 15.0 offspring/female ChV > /cC7. %CV: . *,.0*. 47, <40.0'% IC25 > t007o
Environmental Testing Solutions, Inc. Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: if _____o _ CONTROL - 1 Survival and Reprodtictibn Data Replicate number-Day _1_ 2 3 4 5 6 7 8 9 10 Young produced 0C) c_) _) 0 c). Adult mortality ,. 2 f oung produced c) t I) c) ] l ol o o c
.3 Adlmrtairty Young produced Tooi 'fr c0) _iI Adult mortality 1,_ .] A I , T .
4 Young produced 1 -s Adult mortality I __t C_ 1V_[ j .- Young 6 produced 0 L Adult mortality " L -
- i._ , ' __ .. I ' I -'-
, d ce i, T i' Total young produced 2--S & 21 ~2ý9 z-:
77- - I 3c) 'Final Adult )'1ortality ____ ____ ~ ___ ___ ___ ___ X for 3" Broods . . " " > { >'< ->2".
< - 7 ><
Note: Adult mortality (L live, D =dead) Concentration.-
% Mortality: £Y7o Mean Offspring/Female: -*'.
co~c: 11.3% Survival and Reproduction Data Replicate number Day -1 2 3 j 4 } 5 6 7 8 [ 9 10 I Young produced .Q 6) _) L F7Adalt mortalty 1_. t.- t. I I_._____ mortality 3 Young produced __ Adult mortality S . Young produced ]
.T a) 3t S LA f
_jloýt]aIir Toa. young produced [9 _ _. ,,_, _ _':X 3 ._,_ _ 3_ 2° q S oung produced i Fn Adult l Mortality f__._.._ ____,
-t
{ ., k_.. .__ k... _ote: Adult mortalt'( Young produced Adultimortality
= __
DI=dead)_
.ntr4ol
___i__O,
% e t r C 1 L
lit Total.young produced 2 -Z) Z zR 3Zz &3 28 2!9 z 6 g prouce OfsrigFe ae ",-2.0 Final Adult Ylortality . "- LK 's - K Note: Adult mortality (L live, D =dead) Concentration: -
% M'&ortality: r Mean Offspring/Female: -29.1q
%/Reductionfrom Control: -4, 3'7.
Environmental Testing Solutions, Inc. Species: Criodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: IVTi coNc: 22.6% Survival and Reproduction Data Replicate number . ..
,Day, 1 "2 3 4 5 6 7 8 9 10 2
I fugproduced ral Young produced 0~ 0) { ct I 0) C0 _ C) Adult mortality I L ,_* . "\ .. _T* , 23 Young produced Adul3 mortaldtyd J ) c OI_I C) 0~ 1 -0 1 If C 1_ _ 4 Young produced ! A Adult mortality } __ _ __ ___ _ _._ C ~Young produced ~ IJ c)~o j [
.A dult m ortality ,... .... .. _ " .... ...
6 Yxoung produced (A vA A_ I_ L____ is I_-- Total young produced 2 30 *5 -.. -c ..3 Final Adult Mortaliry L- \ L L L. Note: Adult morality (L live, D dead) Concentration:
% Mortality:
Mean Offspring/Female: :30. ,
% Reduction from Control: -
coNc: 45.2% Survival and Reproduction Data Replicate number Day 4, 5 6 7 8 9 10 1 Young produced ._ _ _ _ ._ _ .( _< _ 10 C_ Adult mortality ._..- ___._ _ _ _ .. _ 1 _] _ \.. 2 Young produced _____ ____ ____C_______ Adult mortality
.3 Young produced __ __ c) ,.l o _ ..
Adult mortality _ _...J.- 4 Yon rduced L~ I LA L4_ __ __ __ _ __ __ _ Adult mortality 6- Voung produced0 [7j7{ ,_ L. J L L_ _ L_ L. J f _. __ 7- " Young produced I 6 Young. produced ____t'I I' ___ "_"_ ____ __ _ 1"_.. _ ,,_ _ ,_ _ -- Total young produced 32- 31\ 3 - Z9 '2 -0 3 0 -3Z, Z9 Final Adult-,Vortali, . L_. \ \ _ . 7 .- 1 ___ ._ j__ Note: Adult mortality (L = liveD = dead) Concentration:
% Mortality: 07.
Mean Offspring/Female: 30. L4 0
% Reduction from Control: - 1. R
Environmental Testing Solutions, Inc. , tS Species: Ceriodaphnia.dubia Client: Sequoyah Nuclear Plant - Non-treated. Date: \\ - co0c: 72.6% Survival and Reproduction Data ___' _ Replicate number Oay 1 2 3 41 5 6 .7 8 10 dAdultmortality [C - E'- young produced 4 '-0, 12 mortality ! uuodut -[ 5 C_ -Young.produced 4 Young produced 1 Final A mo Adult rdultmraity rtality ____ 6}Young produced1 __ __ __ __ ________ Adulthmortality Mo r\ .i "oungpr.d_..d R...eductio f m.__ ._n o - Ib __.. 7 L Youngproduced S' tepr- and dutel,iS D at3 8 Total young produced 03 2- 3A 3t * ' 3t" Final Adult mortalit y - _ . . k.L.
- L. ._ \... _ \.-
Note: Adult mortality (L live, D = dead)
% .Mortality:i Mean Offspring/Female: .3 1.
% Reduction from Control: - I t . 3*7.
CONC:L.100% Survival and Reproduction Data 3 Youngncprtration: ______ _____ Rep!icate number Day _____ _ 1 2 3 4 .5 6 7 .8 9 10 Adult mortality -. L I _ - L L . ,.2 . 3 Young produced f f J II).... Adultmortality L aproduced _ K--- , --
+ _ f.. C_
*Adult mortality _ "_ ._.
6 7 JYOung pro0duced
.Y'oung produced p ol.__
iL TI iS- *
,* 4 4., i11, ,oS Total young produced 31 -'k 30 *- 3 c 0-.. , .. -3"- 21 3 2- 1 Final Adult Mortality ,_ i C '.. __ j . , 1 .-. .
Note: Adult mortality (L = live, D = dead) Concentration: I
% Mortality:, . 07.
Mean Offspring/Female: '31 .
% Reduiction from Control: - 3A 7 o 0'
EnvironmentalTesting Solutions, Inc. Page 5 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: t\-z-o(, .CONTROL - 2 Survival and Reproduction Data I Replicate number "
- Day !_1 2 f 3 4 5 6 7 8 9 10 I 1 'Young produced i _ _ _ ' 0 1 c' Adult mortality1 -i t. -
2 Noung produced _ _ C o) ! K C) 0 3 SAYoung dultm 0r tal *INU produced I
- I _, I C -
-! LI Lt __* __
t _ t _ Adult mortalit" ___ v _. _ _.--- _ L___ L_- __ _ ___ _ __ _ Adult mortality [) D__ o o Fo o o 0 Young produced I 1 " _ _ _" _ S 'A A*dult nmortality Young produced I 1*
- f 1 '" ! V-1 s -- C)-
O IO 0 V- f,v-Adult mortalir, UL*y1 _L _ I 1 Young produced IW ol_,* 1 _c LL ol 1 c o T' 7 Young produced Total young produced Final Adult M*ortality, Note: Adult mortality (L
Žt2 023 1 -'L live, D = dead)
.AL.
-2
* ... v _
2 L.,__ L 30,O 1\. 1 k. AW,,_ a& ._ Concentration.
% Mortality: 0 Mean Offspring/Female: 2. (,
coNc: 100% Intake Survival and Reproduction Data Replicate number Da y p3 1 2 4 5 6 -7 8 9 10 Adult mortality . ,_ ._ I. L 2 Young produced 0 0[ 0 ~I f! 1 k%'i V*.- C Ge 0
£ Adult C1 51 mortality ,I_. L 3 Young:produced 5
AJYun r]rducelt.~~~ 13 Young produced ]L 4
'Z 1 I14 L4 14 2 L'1, Lh v 1d
[C) T y Adult oung mortalityjuj produced *:l 3 0 * -ik' Adult mo rtalit y L -iveD Idead) produced tYoung Totalyoung produced .
.* -- 2a
- 2,,1 "2-ý "Z . 32 ,3 c)
- Final Adult M g,-rtality'I.a Ad l morrtltl:ity k.., k,_ k.. ... , k.. .. .i ,_ k..
Note: Adult mortality (L = live, D = deadl)
.. " ~Concentration:""
% Mlorality: o"7.
Mean Offspring/Female: M 3* .4
% Reduction from Control: -\0.i"7-
.TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Verification of Ceriodaphnia Reproduction Totals Control-I 72.6%
Day Replicate mumber Total Day -1 3 4 5 6 7 8 9 I0 Da y 71 2 13 4 Replicate 5 number 7
'6 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 1. 0 0 0 0 0. 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0
*4 3 4 4 4 4 3 4 3 3 4 36 4 4 5 6 4 4 6 5 5 4 4 47 5 0 0 0 0 0 0 0 0 0 0. 0 5 11 0 0 0 0 12 0 13 11 0 47
.6 9 .11 12 10 10 10 12 10 13 11 108 6 0 .13 11 "12 10 0 11 0 0 11 68 7 13 12 15 14 14 14 12 16 13 15 138 7 13 17 14 14 14 16 15 18 1. 13 149 Total 25 27 31 28 28 27 28 29 29 30 282 Total 28 35 31 30 28 34 31 36 30 28 311 11.3% 100%
DayIRepicate iumber Replicate number Total 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0__ 0 0 0. 0 0 0 u 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 3 5 5 6 4 4 .4 4 43 4 5 4 4 5 4 6 5 6 4 5 48 5 0 0 0 0 0 0 0 0 0 0 0 5 0 12 0 0 0 0 0 0 0 13 25 . 6 9 12 12 10 10 11 13 10 1 11 109 6 12 0 10 13 11 12 11 14 10 0 93 7 13 12 17 14 13 15 16 14 14 14 142 7 14 15 16 15 15 18 14 17 15 14 153 Total 26 28 32 29 28 32 33 28 29 29 294 Total 31 31 30 33 30 36 36 37 29 32 319 22.6% Control-2 Day 1 IT 0 -'., 2 0 3 0 4 0 0 7 Replicate nunber 60 7. 0 8 0 9 0 10 0 Total 0 Day 1 y 1a1* 2
.0 0
1 _ 0 4Replicate 0 5 0 6 number 0 7 0 8 0 9 0 10 0
. o a Total 0
2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 4 4 5 4 4 6 4 4 5 43 4 4 3 3. 3 4 3 5 4 4 3 36 5 0 0 0 0 0 .0 0 0 0 0 0 5 11 0 0 0 0 0 0 0 0 11 22 6 10 12 10 13 11 "11 Ii 12, 10 12 112 6 0 10 10 9 12 10 9 11 10 0 81 7 16 14 15 14 14 16 13 15 17 14 148 7 13 12 12 16 114 13 16 13 14 14 137 Total 29 30 29 32 29 31 30 31 31 31. 303 Total 28 25 25 28 30 26 30 28 28 28 276 45.2% 100%/o Intake Day Dv 1 2 " 3 4 Replicate 5 16 nwnber
~ 17 ~ 8 9 10 Total Total-~ Replicate number
-~1 Day 1 2 3 4 5 6 7 8 9 1 Total
- 1 0 '.0 0 0 0, 0 0 "0 0 0 0 1 0 0 0 0 0 0 o 0 0 0 0
*2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0. 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 4 6 4 4 5 45 4 4 4 5 4 4 4 3 4 4 5 41 S 0 14 0 11 0 0 0 0 0 A 25 5 13 0 12 12 0 11 13 12 12 0 85 6 11 ) 13 0 10 10 12 11 13 10 90 6 0 11 0 0 12 0 0 0 0 10 33 7 16 14 '13 17 14 14 12 15 15 14 144 7 15 13 14 17 13 13 16 14 15 15 145 Total 31 32 31 32 29 28 30 30 32 29 304 Total 32 28. 31 33 29 28 32 30 31 30 304
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic XWhole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statisti cal Analyses Project number: 2906 Reveiwed by: / I Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction fi (0/n) 1 2 3 4 5 6 . 7 8 9 10 (~~%) (offspring/female) variation (/) pooled controls (, Control-1 25 27 31 28 28 . 27 28 29 29 30 100 28.2 6.0 Not applicabli
.11.3% 26 28 32 29 .28 32 33 28 29 .29 100 29.4 7.6 -4.3 22.6% 29 30 .29 32 29 31 30 31 31 31 100 30.3 3.5 -7.4 45.20/u .31 32 31 32 29 28 30 30 32 .29 100 30.4 4.7 -7.8 72.6%/.. 28 35 31 30 28 34 31 36 30 28 100 31.1 9.5 -10.3 100% 31 31 30 33 30 36 30 37 29 32 100 31.9 8.4 -13.1 Control - 2 28 25 25 . 28 30 26 30 28 28 28 100 27.6 6.4 Not applicabl 100% Intake 32 28 31 33 29 28 32 30 31 30 100 30.4 5.6 -10.1 Outfall 101: *MSD= Minimum Significant Difference Dunnett's MSD value: 2.166 PMSD Percent Minimum Significant Difference PMSD: 7.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatinent that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. cluronic toxicity tests w.
Intake: a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Durinett's MSD value: 1.353 Lower PMSD bound determined by USEPA ( 10 1h pe-kentile) 11%. PMSD: 4.9 Uppenr PMSD bound determined by USEPA (901' percentile) 37%.
'The lower and upper bounds were calculated by tie USEPA using 393 tests conducted froln 33 laboratories for Ceriodaphniareproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Ap*plications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency,'Cincirnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 -December 05, 2006 Statistical Analyses Ceriodaephnia Sursival and.Reproduction Test-Reproduction Starn Date: 1112812006 Test ID, CdFRCR Sample ID: V'A/ Sequoyah Nucleaa Plat Outfal 101 End Dte. 121512006 Lab ID: ETS-Eivir. Testing Sol Sample Type: DMR-Dischuge Monitoaing Report Samtple Date: Psotocol: FWCHR-EPA-821-R-02-013 Test Specie": CD-Ceriodaphaia dubh Comments Non-tealed Con,-% 1 2 3 4 5 6 7 8 9 I0 D-Contol 25000 27.000 31.000 28.000 2, 000 27,000 28.000 29.000 29 000 3000 113 26.000 28.000 32.000 29.000 28.000 32.000 33.000 28000 29.000 29000 226 29.000 30000 2.9000 32.000 29.000 31.000 30.000 31.000 31.000 31.000 452 31 000 321000 31.000 32000 29000 . 28000 30.000 30.000 32.000 29000 72,6 28000 35,000 31000 30.000 28.000 34.000 31,000 36000 30000 20000 100 31.000 31 000 30000 33000 30.000 36.000 30.000 37.000 29.000 32.000 Transform: Untransformed 1-Tailed Isotonic Canc-% 'Mean N-0-lean Mean Min Mb>a CV% N t-Stat Critical NISD Mean N-]Mcan D-Contol 28.200 1.000 28,200 25.000 31.000 5.961. 10 30,217 I 0000 11 3 29400 1,0426 29 400 26000 33.000 7.555 10 .1 267 2.287 2.166 30217 1 0000 22.6 A 300 1.0745 30,300 29.000 32.000 3.496 10 -2.217 2.287 2166 30 217 1 0000 45.2 30.400 1.0760 30.400 2&.000 32.000 4.703 10 .2.322 2.267 2 166 30.217 1 0000 72.6 31,100 1.1028 31,100 26.000 36.000 9 520 - 10 -3.061 2.287 2.166 30.217 1 0000 100 31.900 1 1312 31.900 29.000 37000 8.418 10 -3.906 2287 2166 30217 1.0000 Auxiliary Tests Statiotoc Critical Skew Kurl 0 Klcdnogotov D Test indicates nonnal distrnbution (p> 0 1) 0938959122 1.035 0.601190678 0,062444916 Bafletl's Test idicates equal varajtces (p 0004) 11.9811039 15108627224 1lypothesis Test (1-tail, 0.05) . NOEC EOEC Chv TU NSDu MISDp MSB MSE F-Tr.b df Dusnetsa Test 100 >I0 .1 2 166195650.076615449 16.77666667 4.467037037 000559109 5.534 Tteaencetts OsD-Control Linear Interpolation (200 Reacmples) Point % SD .95% CL Skew IC05 >100 ICIO >100 )C15 >100 10 . IC20 . >100 09 IC2.5 >100 0.- IC40 >100 ICSO >100 0.6
.0 0.4 02 0.0 - O
-0.1 . .. . . .. . . .. .. . . ..
-0.2 0 20 40 60 60 100 120 Dose %
Dose-Response Plot 40 35 30 I i ~ ~1T 1-tail. 0.05 level' S25 of significance
- 20 01 5 10 5
so 03
.,4s_ -2806dot.
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 28 - December 05, 2006 Statistical Analyses Ceriodaphnia Survival and Reproduction Tet-Reproduction Staro Date: 11/2912006 Test ID: CdFRCR Sample ID. TVA] Sequoyah Nuclear Plant, Intake End Date: 12/512006 Lab ID: ETS-Envir. Testing Sot . Sample Type: DMR-Dischaoge Monitoring Report Sample Date: Protocol FWCHR-EPA-82t-R-02-013 Test Species:
- CD-Cetiodaphnia dubia Comments: Non-taeated Cone-/a 1 2 3 4 5 6 7 8 9 10 D-Contool 28.000 25.000 25.000 20000 30.000 26.000 30.000 28.000 280000 28.000 100 32.000 28.000 31.000 33.000 29.000 28.000 32.000 30.000 31.000 30.000 Transform: Untr2ansfornned I-Tailed 3otonic CaoC-% 1,1ean - N-Mean Mean Min yalx -CV% N I-Stat Critical MSD Mean N-Mean, D-Contmol 27T600 1,0000 27.600 2.5000 30000 6.436 ,i0 29.000 1.0000 200 30.400 1,10t4 30,400 28.000 33.000 5.634 10 -3.588 1.734 1.353 29.000 1.0000 AUnxiiary Tests Statistic Critical Skeo Kurt Shapi;o-Wdk's Test indicates normal disbtibution (p > O.01) 0.919082224 0.868 -018624647 -0.98833966 F-Test indicates equalvawatiacc (p = 0.92) 1,075757623 6.541089535 1lopathes io Tet (l.tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no sigrficanit difTerences 1,353112957 0.049025832 39.2 3044444444 0.002101165 1, is Treatments vs D-Contsol Linear Interpolation (200 Resamples)
Point % SD 95% CU. Skew IC05 >100 ICIO >100 ICI5 >10(0 .0 IC20 >100 09 IC25 >100 0o IC40 >100 tC50 >100 00 " 0.8 0o4 0.3 0.2 01 00 ,
-01 "..' - *
-0.2 0 20 40 60 80 100 . 120 '
Dose % Dote--Rlponse Plot 35 30
... 1-tail. 0.05 level 25 of significance 0
120 5 0 0 --. U)
. sqn_ 1-28-06dato
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphiiiadubia Daily Chemical Analyses Project number: 2906 Reviewed'by: Concentration Parameter . Day 0 Day I Day 2 , Day 3 Day 4 Da 5 Da .6
, Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Fina pH (SU) 7.69 7.35 7.75 7.21 7.59 7.27 7.821 7.36 7.89 7.50 7.90 7.34 7,65, DO (tng/1L) 7.7 7.8 7.8 7.5 7.71 7.7 7.61 7.71 7.7 7.8 7.8 7.8 7.8 Conductivity (lamhos/crn) 318 318 3507304 300 309 Control Alkalinity (mg/L C.C 3 5859. 8 Hardness (mg/L CaCO3 ) 92 86 90 Temperature (°C) 24.7 25.1 24.7 24.7 24.6. 24.9 24.8 25.2 24.7 24.7 24.8 24.8 24 6 2 S pH.(SU) 8.00 7.35 7.43 7.20 7,28 - 7.26 7.33 7.35 T44 7.49 7.66 7.34 738 _
11.3% DO (mg/L) 7.7 7.8 7.8 75 7.6 7.7 7.8 7.8 78 7.8 8.1 7.8 8.1 Conductivity (limhos/cm) 301 304 295 286 .289 284 29.0 Temperature ( °C) 24.7 24.8 24.8 . 24.7 24.8 24.6 24.8 25.0 24.7 25.0 24.8 24.7 24.6 PH (SU) 8.00 7.35, 7.42 7.20 7.27 727 7.32 735 7.43 7.50 7.64 7.33 7.381 DO (mg/L) "1 7.7 7 8 7.8 7.5. 7.6, 7.7 7.8 7.7 78 7.8 8.1 7.7 8.1 Conductivity ( ,mhds/cm) 287 2891 282 275 275 273 277 _Temperature ('C) 24.9 24.8 24.8 24.7 24.8 24.8 24.9 24.9 24.7 25.0 24 9 24.8 24.7 p PH (SU) 8.01 7.35 ' 7.40 7.21 7.25 7.25 7.29 7 34 7.43 7.51 7.58 7.32 7.36 ! DO (mg/L) 7.7 7.8 7.8 7.4 7.6 7T6 7.9 7.7 7.8 7.8 8.2 7.7 8.1 4Conductivity (lpmhos/cm) . .260 261 256 260 248 251 252 _Temperature ( °C) 24.9 24.8 24.8 24.9 24.9 24.7 24.9 25.1 24.7 25.1 24.9 24.8 24.7 pH (SU) 7.99 7.34 7.39 7:20 7.23 7.26 .7.27 7.34 7.38 .7.49 7.56 7.35 7.35 DO (mng/L) 7.7 7.9 7.7 7.3 7.8 7.5 8.0 7.7 7.8 7.8 8 2 7.7 8.1 7Conductivity (pmhos/cm) 234 225 222 I 218 215 218 222 72.74 Temperature ('C) 25.0 24.8 24.9 24.6 24.9 24.7 24.9 24.9 24.8 24.9 24.9 4 24.7 pi t(SU) 7.971 7.36 7.40 7.22 7.201 7.26 7.22 7.33 7.38 7.49 7.49 7.35 7.321 DO0 (ing/L) . 7.71 . 7.9, 7.7 7.2 7.91 7.5, 8.2 768.01 7.71 8.21 7.71 8.11 Cotnductivity (Punmbos/c) 189 19 192 185 ;7 18808 1oOV/0 Alkalinity (ngIl. CaCO,) 66= 9 66 66 Hardness (mg/L CaCO3 ) 78 86 80 Total Residual Chlorine (rag/L) <0.10 <0.10 <0.10 Temperature (C) 25.2 24.9 24.9 24.6 24.8 25.01 25.0 24.8 24.9 24.9 24.91 24.7 PpH (SU) 7.95 '7.35 7.39 77.21 17 . 7.23 '7.20 . 7.41 7.32 7.50 7.44 7.33 7.32 DO (mg/L) 77 8.1 7.9 . 7.6 .9 g 7.6 8.2 7.8 8.0 7.8 8.2 7.8 8.21 Conductiv'ity (tmhos/ctin) .191 192 191 4189 100% Intake Alkalinity (mg/L CaCO 3 ) 65 67 64 Hardness (mg/L CaCQ3 ) 80 84 80 Total Residual Chlorine (rfig/L) <0.10 <0.10 <0.10 Temperature (C) "25 1 "24.1 24.7 24.8 25.0 24.7 24.9 25.0 24.8 24.8 24.9 24.7 24.61
Envir6nmental Testing Solutions, Inc. t'age o ot/ Envir6nmental Testin2 Solutions, Inc. ~age 0 or I Species: Ceriodaphniadubia Date: I , -2z" -0 ,k Client: Sequovah Nuclear Plant - Non-treated Daily Chpn,jc,'r-u, Day
* .Analyst gaP** .. ' I"gk Concentration Parameter '1
- CONTROL pH (SýU.) .W-___l DO (mg/L)
Conductivity Alkalinity (mg CaCO3!L)
- Hardness (mg CaCO 3 /L)
Temperature (°C) 114.1 15. t 7_2 .'.1 1,-k _,, PpH(S.U.) 8.0 0 9.3 . 'T-13 1.2o ?.9d 11.3% DO (mn/L) Conductivity (prmhos/cm) 3D ________ Temperature ('C) "A ._.< . , Z.1 " . .0 __ "._, pH (SýU.) (5.Do "p.35. ý-%- 2I, 22.6% DO (mg/L)
- 0. ". ." ,
- Conductivity "+
(pmhos,/c )_,_- -_ ;_ - _ Temperature (°C) -..- __ ..q "_ - _ _ ,a _j_1 _A __ . pH (S.U.) o- 35.Z 21 . . 5.- 45.2% DO (mg/L) Conductivity P"" , " (pimhos/cm________ ____ Temperature (OC) 1.- L ,'4
-"A -ZIA7"1
-31
_I_ _" pH (S.U .) -q . .20 2 9*-,,q (, 72.6% DO (mg/L) D___.-.,_, Conductivity (ýirmhos/cm ) M. Temperature(°C) 2('Q 1A ______ , 1".* . "-V.-A PH (S.U.) ý ,qo 2 lO q 100% DO(mglL) DOmhos/cm) " .'._ . Alkalinity (mg CaCO3/L)
'Hardness " " -
(mg CaCO3 /L) TR chlorine. _(_W/L)
*
- 4o IC*O )O Temperature (°C) ZS. 7.-
pH(S.U.) .-. 5 3T . 9.] '- 100% Inlake DO (mg/L) Conductivity (.imhos/cm) 19l Alkalinity (mg CaCO 3/L) Hardness
-(M CaCOAI)
- TR chlorine e(m_.(C.
gL) " (C) <0.)I 0. . ___ __ _ _ Tempeature Z'---2*, "2Cb*q7 z)* o i',q*;
'-.7 Initi Final I Initial I Final I Initial 11 -Final I
Environmental Testing Solutions, Inc. Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date:. 3______ 4______ 6 _________Analyst Concen- Parameter 7, 7. tration , . 1 t~~- CONTROL pH (SU.) e)2- 'q4 Sb '(OD Cý ~ ~ 1 DO (mg/L) Conductivity - Alkalinity (mg CaCOfL) Hardness (mg C a C0;L)TI __ _ _ Temperature ('C) -LA) LL7L LS -2 7 .'I
-z.1 Z.4.. R 11.3% DO (mg,/L)__ __ _ __ '
Conductivity . -o 0 __ __ _ Temperature ( CQ 0h __ __ Ž -I__ TAS. -,A. t _ _ _ __H~ (S. _U.)__ _ _ _ 3 2-_.__q_5_ 22.6% DO (tn/L). _ _ _ ___ Condu~ctivity .- ~ (Pmrhos/cm) ____________ U 3____ _____ __ _ _ _ Temperature ('C) ~ 1A~ A1 -IS -0 -LA-ctý . A -V1 45.2% DO (mg/L) h . '.S . Conductivity ..- _____Temperature ('C) ~ A1 -sl 2l~ 2 1 9j ~Z. 24.S 72.6% DO (mg/L). eo.(95 Conductivity ~ 8 ~ . . -~*~. __ __ __ Temperature (T) ______ IQ.__140 .21.' 2Nc, *-rA* 7-4l . pF1(S. U.), _D_ 5_ Q __ _ __ _ _ __ _ _ 100%/ DO (rmg[L) 8.- C((, X-2Z9? .C ( nihos/cm) e 9 (mg CaCO 3 /L) TR Chlorine (mg) z.404 Temperature('C .0 -ZSQ C) -lqa . .Z-.ý Q 2 PH (S U.) '3.,41 1 Iý5 11z I Qqt~~~/ cý.32 1 .S2 { .'ý 100% DO (rg/L) Ic' -0 le .2-II
ý.e I Intake Conductivity (Pmhos/cm)
Alkalinity (mg CaCO 3 /L) kOlee Hardness m(g CaCO 3/L) TR chlorine (m L) 90~ Temperature (°C) l l II 2q.7 IZ- -~l I[JŽZL H * *
,_'j 4 I
.I In l IIit Final Initial I Final j Initial I
Final J Initial II ~ nat initial ___
Environmental Testing Solutions, Inc. 'rdý;r- I U1 U Eniomna Tesin Souios ic r-u~IU Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas Client: TVA County: Hamilton Facility: Sequovah Nuclear Plant Treatment: UV-treated NPDES #: TN 0026450 Outfall: 101 Project #: Zcibto Dilution preparationinformation: Comments.- Dilution prep (%) 11.3 226 45.2 72.6E =100 Each concentration was treated Effluent volume (mL) 282 5
- 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 3.$.,* . 1370 685 0 to remove pathogenie Total volume (mL) 2500 2500 2500 2500 2500 interferences.
Test organism information: Test information:. Organism age: Z"M1'l - . .(3t 0e; 2zS.Is Randomizing template: 6i.t Date and times organisms - to, -m tt,:,CO Incubator number: were born between: _ _. Organism source: ASS ý^Vt.A l\ drAemia 4r lot number: 6& 1-2ct U Transfer bowl information: pH = Temperature '°C Total drying time: - - zV __.___.' _ _ _ Date/Tim e in: -.-c ., \.,, 2.O Average transfer volume: Date /Time out: Iz-C,-c,, ko'Z. Oven temperature: I k__ ..,_ Dailyfeeding and renewalinformation: Day Date Morning Afternoon Test initiation, tN[HS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 1 1"16,- ot7. ,o 2 3 DDbO .A LIJ IS 0z t,- -oo -A. i n to 3 ) 2 o2 - ( )O.O
, 06 V'.S
- 21. 1<* 1A . 1-2.,6- Uo,: 1"3 6,t1 2 . 0- 1 -* 0& a 4 z1-o .wo6 0z-5 0 153 1 . so \-126- U")IS9.o Z0 14oO7--
56 lZ - l . O o1 r-C.4 SCh$O ,z-o'~-\(, i 0O.o*0.o ý*o 7 2 -. NO . . , . Control information.: Acceptance criteria Summary of test endpoints:
% Mortality:
Average weight per initial larvae: o7'. O. U I l _20% 7-day LC 0 NOEC 10507. I*O7. Average weightper surviving larvae: O." 1 Ž 0.25 mg/larvae LOEC > I1(. O7 ChV
>)O7~
Ic,; I I1,
Environmental II Testing-Solutions, i Inc. Species: Pimeplhales pro- elas Date: t/.; eDv Client: TVA / Sequoyah -Nuclear Plant - UV-treated Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E FI G H I J IK L 0
// ) /0 to 1 L) i( ) /0 jo IDLt) JI,U D/0 ' /C 10 to /0 1/ 1 10 IC) 10 'C) 2 /0C0 /0 0 /0 /C/0 IC) /0 /0 3
10 (0. .1o /0 /0 /0 /o /0 /6 10 10 4 iD 10 '0010 10 10 C I C0 1 0 10 I0 10 1/0 0 /0 10 1 /0 /0 /0 i0 /0 /0 6 I0 0 ;0
)0 1t tO /0 /0 / 0 /O /0 10 1O /0 (0 /0 /0 tO 10 (03 1 (0 to A Pan weight (mg) -LColor L identifica
_-Yýtion:
ýCI4v.-q 3.q c 13.qk49 q.3) tq.*:cD ly-17o I"{.q 16.31 I1ý ilq 1q.`70 114.33 1'.S) knaly'st: L%';?)
B (ra =g)Pan + Larvae weight o. LlC-10.* a .,4q a w B.18 a .qq Do. C1 0 -90 'oa a ,S'7 n oIG . )o11 0 1 1.13 a lk30 6 Analyst: vo. Larvae weight (mg) A - B Weight per initial number 0\, A,) of larvae (mg) C C/Initial number of larvae NO A0o 0* D" 0 0 Na cz)- 0' c" Average Percent weight per reduction 0.1~-Z~~'s initial from control 0 number of (/ larvae (mg_________ Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, srnt unusually small, 1g = unusually large, d&r = decanted and returned, w = wounded. Calculations and data-reviewed: f 4j-Comments:
Environmental Testing Solutions, Inc. Pace 3 of 6 Species: Pimephlalespromelas Client: TVA / Sequoyah Nuclear Plant - TV-treated Date: Survival and Growth Data Day 45.2% 72.6% 100% _ _ _ N 0 P Q R S T U V W X AL) /0 ) ) IO /0 /o /0 IC) A.) /R /0 2 6 to /0 to " /D /0 0 /0 ./0 /0 /0 10 3 0 to to (0 1'0 t'0 (0g
' O O /
40 /0 (0 (0 /0 (0 (0 / t C_ 10 I( tO 5/0 /0 /0 (0 to 1 /0 C) ,'1C to t 6 / 1/0) /0 /0 (0 /0 /0 /C /0 /0 "0 /O C 1 /0 C) 1o /1 /0 (b /0 /o /0 A = Pan weight (mg) Color identification: Ie +ro.q /3.90 Iq lI 1q31 JH3 1q98 Jq99 Iq31 Analyst: : B = Pan - Larvae weight (mg) ao.aq ao.,a,.S3 i0.- ao.Z aa.c *o.(Daa' at.r6 aa.ti a&0.M9 Analyst: L Larvae weight (mg) = A - B Weight per initial number of tarvae (mg) ' $) 4
ýweight per initial
.reduction from control -
larvae (mg*) Comment codes: c =clear, d dead, fg fungus, k killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. a
... "Calculations and data reýi~ewed:
Comments:
Environmental Testing Solutions. Inc. Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: 1 t -&L>X, Survival and Growth Data Day 100% Intake Y Z AA 0/0 JL) /c / 1 1(0 /0 /0 /0 2 (U /0 '0 '0 3 (c /0 /0 (0 4 /b '0 'O '0
/0 C0 /0 /Q 6 Q to to 10 A IPan weight (mg)
Color identification: cr___ t V1uacs q ql.(S I i.l 133 Analyst: kj.. B = Pan + Larvae weight (mag) &.Ha** l3 ** Analyst: U4-t Larvae weight (mg) = A- B Weight per initial number of larvae (mg) %
= C / Initial number of larvae. ý"O '. , ' ,o Average. Percent weight per reduction initial from control 0.t'4 2. 7.
number of (%) larvae (mg) .. _" Comnent codes: "c clear, d = dead, fg fungus, k- killed, m= missing, sk sick, sm unusually small, Ig = unbsually large, d&r decanted and returned, w = wounded. Calculations'and data reviewed: Comments."
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated. November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromnelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses. Project number: 2906. Rer'eroed bY: A 4io-. Not for Compliance Assessment, Internal Laboratory QC _'- Co
... ro.rion (".) Rrplicrl loirot number
- or inorrorrrbor
. ol d l.. A P.
Pan ithn B = P'n 1 1 *-, L'"o, rrgtrhi (ml) Wnjiht I Suoiiotn M -r .eight I Coffei nol o.(a!rwi-n WVright I Initial nmbbMr -l-n roiro)1 Otoon oidht / conmcieni of Perenrtd.o- I brine , tn ) (i~at 1 ooureirrnhl = A- B nu obenor lrr loot) me S url iio n unm ber orf f h"' ,= ," ' *r br-ofri coo nrc 0/0ib (va° lnr lib ono t r 0g o ubimb or.....1, , rOn.ro I 1 °/.b I.rv e (mg) . *r.I.n.-.=..)(Z) minor (me) p.r l .I.r.,.r.fr. A 10 10 14.67 20.32 5.65 0.56S 0.565 Control R5 10 10 13.99 20.44 6.45 0.645 0661 12.3 . 0.645 100.0 0,661 12.3 Not applicab C t0 10 1349 20.18 6.69 0669 0 669
- 1) 10 10 1431 21.94 7.63 0.763 0.763 E t0 10 14.60 21.05 6.45 0,645 0 645 11.3/ F. 10 10 14.76 22.14 7. 038 0.675 6.3 0.730 100.0 0.675 6.3 -2.2 G 10 10 14.09 20.70 6.61. 0.661 10661 H 10 10 16.31 22.87 6.56 0.656 0.656 I .- 10 10 14.14 20.11 5.97 0.597 0.597 22.6 'K J 10 10 14.70 20.72 6.02 0.602 0.635 8.2 0.602 100.0 0.635 8.2 3.9 K 10 10 14.83 21.13 6.30 0.630 0.630 L 10 10 14.5 1 21.60 7.09 0.709 0.709 M 10 10 13.90 20.24 6.34 0.634 ' 0.634 45.2% N 10 10 14.78 20.82 6.04 0.604 0.588 8.9 0.604 100.0 0.588 8.9 10.9 0 10 10 14.70 19.83 5.13 0.513 0.513 P 10 1.0 14.31 20.33 6.02 0.602 0.602 Q 10 10 14.23 19.65 5.42 0.542 0.542 72.6 R 10 10 14.98 20.81 5.83 0.53 0.585 6.2 0.583 100.0 0,585 6.2 11.5 S 10 10 14.99 20.82 5.83 0.583 " 0.583 T 10 10 14.31 . 20.61 6.30 0.630 0.630 U 10 10 14.64 21.01 6.37 0.637 0.637 100' V to0 10 14,71 21.76 7.05 0.705 0.678 8.7 0.705 100.0 0.678 8.7 -2.7 Nlo t0 15.23 22.71 7.48 0.748 0.743 X 10 10 14,26 20.49 6.23 0.623 0.623 Y t0 10 14.95 21.14 6.19 0.619 0.619 100%/l.ak Z 10 10 1,4.08 20.16 6.00 0.60 0.644 5.6 0.600 100.0 0.644 5.6 2.5 AA 10 10 14.66 . . 21.33 6.67 0.667 0.667 BB 10 10 14.33 21.15 6.82 0.682 0.682 1 1 1 1 Outfall 101: MSD = Minimurn Significant Difference Dunnett's MSD value: 0.0949 PMSDI Percent Minimum Significant Difference PMSD: 14.A PMSD is a measure of test precision. Tire PMSD is tho rminimumnperceno difference between the contbol rid treaLment that can be declared stalistically significant in a whole effluent toxicity tost average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a loxicatt reduces Pimephales growth by 15.5% fhom the control (determined IhMoug lnlake: reference toxicant test/iog).
Dunonel'u MSD value: 0.0066 Lower PMSD bound deternrined byUSEPA(IOth percentile) 9.4%. -PMSD: 13.1 Upper PMSD bound deternined by USEPA (90th percentile) - 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted forn 19 laboiatories for Pimepholes growth in chronic reference Ioxicant tests. USEPA. 2000. Understanding and Accounting for Method Variabibty in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Ebotination Prograin. EPA.33-R+O0-003. 1.S Ensitorntntal Protection Agency, Cincinnati. Ol.
UV-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growtlh Start Date: 11/28/2006 Test ID. PpFRCR Sample ID: TVA / Sequoyah Noclear Plwat,Outfal 101 Enct Date: 12/5/2006 Lab ID. ETS-Envir TestuIg Sol, Sample Type: DMR-Discharge Monitoing Report Sample Date. Petocol YWCHR-EPA-821.R-02-013 Test Species: PP-Pinephales promelas Cisents UV-to ated Conc-% 1 2 3 4 D-Control 0.5650 06450 0.6690 0.7630 11.3 06450 0.7380 06610 0.6560 22.6 0.5972 0.6020 0.6300 0.,090 45.2 06340 0,6040 0.5130 0.6020 72.6 0.5420 05830 0.5830 06300 100 0 6370 07050 0,74B0 0.6230 Transforma: Untransformed I-Tailed sotnic Conc.% Mean N-Mesa Mean Minm Mx CV3/o N t-Star Critical . ISD Mean N-Mlean D-Contnol 0.6605 1.0000 06605 0 5650 07630 12,343 4 0.6678 1.0000 11.3 0.6750 1.0220 0.6750 0.6450 0.7380 6.301 4 -0368 2410 0.0949 0,6678 10000 22.6 0.6345 09606. 06345. 05970 07090 8.155 4 0660 2410 0.0949 0.6345 0,9502 452 0.5883 08906 0.5883 0.5130 0.6340 8.884 4 1.835 2.410 0.0949 06170 0.9240 726 0.5945 08049 05845 05420 0,6300 6.154 4 1.930 2410 0.0949 0.6170 0.9240 100 0.6783 1.0269 0.6783 0.6230 0.7480 8.654 4 -0.451 2.410 0.0949 06170 0.9240 Auailiary Test5 Statistic Critical Skew Kurt Slsapso-Wilt/s Test indicates normal dist-bstion (p > 0.01) 0.98408103 0 884 0.220567588 -032046338 Boltett's Test indicates equal &ariances(p = 0 82) 2.173702478 15.08627224 llyttthesia Test (l-tail, 0.06) NOEC LOEC ChV TU NaSIua MlSDp MSB MSE F-Prob df DInetrts Test 100 >100 I 0,094887324 0.14365984 0007069367 0.003100361 0.090179361 5, 18 Ttnatments vs D-Control Linear Interpolation (200 R.esanplts) Point % SD 95% CL(Exp) Skew ICOS 22-778 ICIS >100
>100 10o IC20 >100 09-2C25 ->100 0.8 IC40 >100 IC50 >100 07 2 o.5 o0.4 03-0.2 01.
0.0
-0,t 20 40 60 30 1tO 120
,Dos. %
Dose-Ileap-ne H'ot 0.7 006 T 1-tail, 0.05 level
.................. ... ......... .. ...... ... .... ........ ot signilficance 054 04 0.3 0.2 0.t 0
cv
-6 0
- aqr;_l1-28-06dota
UV-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Surviv-l T"s1-7 Day GrIswth Start Date: 11/28/2006 - Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plaunt,Intake End Date 12/5/2006 Lab ID: ETS-Envir. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocou FWCHR-EPA-821-R-02-013
- - Test Species: PP-Pmsephale promelas Comcents: LtV-teated Conc.% 1 2 3 4 D-Control 05650 0.6450 0.6690 0.7630 10e 0.6190 0,6080 0.6670 0,6820 Transfornm: Untransformed 1-Tailed h.Iotnic Con-./ Mean N-Mean Mean Mlin Max CV% N t.-Stat Critical MSD Mean . N-Moan D-Co-tol 06605 1.0000 0.6605 0.5650 0.7630 12.343 4 0.6605 1.0000 100 0.6440 0.9750 0.6440 0.6080 0.6820 5.594 4 0.370 1.943 00866 06440 09750 Au siliary Tes U Statistic Critical Skew Kurt Shapiro-W ilks T,t indicates noural di.stibot ion (p > 0.01) 0.98164469 0749 0.198999913 0.957942952 F.Test indicates equal variances (p = 021) 5.120441914 47.46722794 Hypothesis Test (1-tail, 0.05). MSDu MSDp M1SB MISE F-Prub d[
Hlomoscedastic Test indcates no significail differeuces .006598793 0.131110965 .0005445 0,003972167 0,723920584 1, 6 Treuamerts vs D-Contol Lintar Interpolation (200 Renamplns) Point P% SD 95% CL(Eop) Sk - IC05 >100 ICIO >100 IC15 >100 1. IC20 >100 0.9 IC25 >100 IC40 Ž100 - 08 IC50 >100 07 -
&0.4 02 0.2
-. 0.1 0.0 . . . ' .' ,-
0 20 40 60 80 t00 120 Dose % Doe-Response Plot o05 07
~04o 6 1-tail, 005 lecel of significance 005 t*04 02.
O0 0 0L) sqnl 1-2S-06data
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimnephlalesproinelas Daily Chemical Analyses Project number: 2906 Reviewed by: Concentration Parameter Dy0 D, Diy I Day2 Day3 Day4 DayS Da y6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Fin, PHt (SU) . 7.91 7.31 7.40 7.11 7.42 7.14 7.25 7.18 7.38 7.15 7.44 7.14 7.37 DO ( g/L)8 7. 8. 7. 7.7 7.4 7 7.7 7.7 7.0 8.0 7.8_ 8. Control Conductivity (priihos/cm) 3317307_304 __295 294 I I294 298 Temperature ('C) 24.9 24.7 24.6 24.5 24.8 24.9 24.8 24.6 24.8 24.4 24.9 24.2 24.7 pH (SU) 7.91 7.28 7.40 7.08 7.41 7.14 7.25 7.16 7.36 7.15 7.46 7.13 7.37 DO (rag/L) 7.7 7.9 . 8.1 6.8 7.7 7.4 7.7 7.5 7.8 7.0 8.0 7.8 8.0 11.3 0 Conductivity (phrnos/cm) _305__299 291 290 291 288 291 Temperature ('C) 24.9 24,5 24.7 " 24.7 24.9 24.8 24 9 243 24.8 24.3 25.0 24.1 24.7 pH- (SIJ) 7.90 7.28 7.39 7.07 7.40 7.13 7.25 7.16 7.36 7.14 7.46 7.13 7.36 22.6% DO1)(rag/L) 7.7 7.7 8.1 (.9 7.7. 7.4 7.8 7.5 7.9 6.9 8.1 7.7 8.0 Conductivity (pimhos/cm) " 290 276 284 276 277 275 279 Temperature ('C) . 24.9 24.8 24.8 24.5 24.9 "24.8 24.9 24.3 24.8 24.3 249 24.3 24.8 pH (SU) . 790 7.23 7.40 7.05 7.37 7.13 7.24 7.17 7.34 7.10 7.45 7.13 7.36 4DO,(raZ/L) 7.8 7.6 8.2 6.8 7.8 7.4 7.8 7.5 7.9 6.9 8.1 7.5 8.0 Conductivity (jtmhos/cm) 261 258 254 263 254 253 255
,,. Temperature ('C) 25.0 24.6 25.0 24.6 24.9 24.9 24.9 24.4 24.8 24.4 24.9 24.2 24.8 PIH (SU) . 790 7.29 7.38 7.06 7.37 7.13 .7.20 7.19 7.32 7.15 7.42 7.0)9 7.33 72.60/%7 . /. DO (rag,/L) 7.8 7.5 9.1 6.7 7.8 737.9 7.4 7.9 6.8 8.1 TS7 8.0 Om/
Conductivity (iamhos/cm) 2_30 223 223 220 218 220 224
,ii _ Temperature ('C) 25.1 24.6 25.0 24.6 25.0 24.9 24.9 24.4 24.8 24.5 24.9 24:2 24.8 pH (SU) 7.92 7.29 7.37 7.06 7.35 7.12 7 19 7.18 7.31 7.10 7.42 7.20 7.34 100/ DO (mg/L) 7.6 7.4 8.1 6.6 7.8 7.4 7.9 7.5 7.8 6.8 7.9 7.4 8.0 Conductivity (pm9os/cm) 194 192 194 190 191 19% . 193 Temperature (°C) 25.1 24.4 25.0 24.6 25.1 24.9 25.0 24 5 25.0 . 24.4 25.0 24.2 24 8 pH (SU) 7.91 7.25 7.38 6.96 7.16 7.12 7.16 7.18 7.28 .. 714 7.37 7.15 7.31 100% Intake -DO (rag/L) 7.61 7.6 8.0 6:7 7.9 77.5 8.0 7.5 8.0 . 6.9 8.0 7.5 8.1 Conductivity ".rnltmos/cnmJ 195 II 185 187 187 188 187 190
_Temperature ('C) 25.0
- 24.41 249 24.6 24.9 24.7 24.9 . 24.3. 24.8 24.5 25.01 24.3 . 24.8
Environmental Testing Solutions, Inc. rage ) or rage or a Environmental Testing Solutions, Inc. Species: Pimephalesprornelas Date: I.ý,C40 j /, Client: --TVA / Sequoyah Nuclear Plant UV-treated Daily Chernistry: Day
.Analyst ~g.* Vib-L
*E,. ** * *S.... *t' Concentration Parameter 9, U - . '
CONTROL pH (S.U.) - " 9__. 0 2-2.4 ".Ii+/- DO (mg/L) .C' '3. S. * "3.' "4 Conductivity (pimhos/ M)____________ _,._ Temperature ('C) .i ".
-VA. _1-L_ -,., _ _ __ t_._
pH (S.U.) _ q._A_"__, oc_ _ _--_1 11.3% DO (rng/L) Conductivity ,0-6 .t "'= ', - (Pmnhoslcm) at Temperature( 0 (' C) .Q- Z ,-l1 _ ______.______. pH (S.U.) "9o 2e9- ____ _ 1/2Y 9.,40. 22.6% DO (mg/L)17 Conductivity , ,, . (pmh bs/cm) Aq0 o,
- Temperature( 0 C)
(.Q "_ _. 1'-- .R _-.. " __ 14 .__"_q., pH(S.U.) . .5 q.70 q.34 9.13 45.2% DQ (mg/L) 7- {P. e B"(pmhos/cm) R _ _Temperature ('C) 'tIS*O " .L, ;1*o - "LAq 1 2q.q 1_-1. pH (S.U.) q..,O 9, 29 ').3(9 ao , 94,3 72.6% DO (mg/L) ,{ t3 4.3 Conductivity .r, 309 (ýimhos/cm) Temperature ('C) zV- I V_ _. ___75 1-kqA. 2-. __-"- .0 0p1 (S.U.) 9-.03 . ý.a - 9.')q* - Z 100% DO (mg/L) , .. 1.I (o " 1.. C onductivity 101 .. [q. "
'(jInhos/cm)_________________
Temperature (°C) 2S.1 "ZAq. ?S2.0 "ql, ,, _ 2- .9 pH (S.U.) _ .___ _ - _- 9..3, ,, ,ý "_ _- 100% Intake DO (mg/L) '7&, - ,D ,. Conductivity - r ( mnhos/cm) l1'*5.e1 } Temperature ('C) 2S. 0 22,4. 1_.'_ _ . _, ___,_2q.__ "-,-I Initial Final Initial Final SInitial Final ________ I - &
Environmental 'Iesting ý)oiutiions, inic. Species: Pimephalesprotnelas Date: - l!'cQ6O0' Client: TVA / Sequoyah Nuclear Plant - UV-treated DaN 3 4 '5 6 Analyst I t~L",(-L Vla- l)k-Concen- Parameter : *, . tration Ih-MM.-i CONTROL pH (S.U) ."4q -3 DO(mgL) 0 L Conductivity rnMhoslcrn) Aq Temperature (°C) - -iýAA4 VA A Z - "iýsR ..- 'L A
-pH (s.U,) .. Io. h-a 7 3(.-C "is r)q .."0.13 7 75L ,:z 5
11.30/o DO (m*/L) q.0 .*" Conductivity ( mlios/cmn) Temrn erature (°C) 7 ,"4.. .A. 2L. 3 -W \ "2.k .- I " -tH, pH (S.U.) q.0 . 4.lq q. .. , 52.6% DO(Mgn L) 9.ý ' !
,5l,..
Conductivity Temperature ('C) "t'L. q. " --. "2.. *. _ .2 4 5. 2 %/ PH (S.U.) (DOrnhos/crn) (rag/L) A4,Q(
.41
,* Qq I~ )
g.$O ** ( mi0hos/cm) Tern erature (°C) ,A21. 2 -.- ZP. 'zq. . .& Z -I. Conductivity pH (S.U) U..)o q . / "9*5 .LZ 9 " 434 ,3 72.6% DO(mg Conductivity . Temperature ('C) , ." , "-L.5 7.0 ." .. Zq. k
-. Lq. _
pH (S.U.) 9 "- I.1O 'ý,.2n -4.34 "9.TT (Mhos/cmn)Il 100% .DO (mgfL) .b.9 " 8 . "4. Conductivity pH (Vmhos/cm) . Sal it( Iiil Fia ntal Fnl Iita ia Temperature(QC) ,1%.0 -q, S5.0 "-.2 2-1. 2- q, 2 ,-3 Initial Final Initial Finn] Initial Final Initial Final
Lai1 V 11 k'Jl.II lIta Ii I v2~LjIII -)UIU" v- I Pagye 3____ Page I__ of . Total Residual Chlorine
* (EPA Method 330.5)
Matrix Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Jon Meter Analyst . A _. Iodide reagent: ,J A " Date analyzed Acid reagent: -i ,Pq7 Calibration: Reference! standard"< :" number
' """ .0.10 mg/L 5 N* -sm:ýs*
1.00 /L Note: For samples with a residual chlorine of > 1.O'mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: t Reference standard True value (TV) Measured value (MV) % RS =MV/TV x 100 number (mgfL) (rag/L) (acceptable range = 90 to 110%)
.. S 53-3 0.50 Duplicate sample precision:
Sample. Sample ID Sample characteristics Residual chlorine %RPD = ((S - D) /[(S+D)/2i) x 100 number (mg/L). (acceptable range 10%) ILIc,6t~po~ U~-,lu-x-SPnoLfloz ' ~ctgp S Qljo Duplicate D /-c03&?5 __.. Sample measurements: Sample Sample ID Sample characteristics Residual. chlorine number (mg/L) Blank (should be = < 0.10tg!.) 10o :"/. C)I\.z7.o7 - . 0 (AoLQm-. C, ,- o 9 o3
,-1, --rS J- 1J1 U-C0o_,
_t ."0 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Reviewed by I "= -I= Date reviewedI dl1-2 0G
rtnvlrunmeniat t usung ovultiujll., liRl. Page ___ Page I of Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pWion Meter Analyst 6Li . Iodide reagent: )ý0,*3 Date analyzed -6 ., Acid reagent: I&J (2-_ "Q Calibration: I 010 mg/L _. 1.00,mg/L Reference standard number f) Note: For samples with a residual chlorine of > 1.0 rng/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (NIV) % RS = MV / TV x 100 number (mg/L) (mrg/L) , (acceptable range= 90 to 110%)
,is 5 0.50 0 . LCq.Zq Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD ((S - D) /[(S+D)/2]} x 100 number -" (mg/L) (acceptable range = 10%)- Duplicate D -K1ýO Z Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number ___mg/L)
- 4 .,1A Blank (should be = < 0.10 mg/L) 17_._.0__0_
1A-5ON,3-
"e-e"i 101 * ~c~t&oc kQa*. " 0O~c)3o33 Note. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV/TV x 100 number (mg/L) (mg/L) (accep table range = 90 to 110%) 505 ? 0.A 0.50, Reviewed by r Y Date reviewed "
lnvironmentai iesting mOIUtions, Inc. Page _q Page _ _ of' Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accurnet Modei AR25 pH/Ion Meter Analyst I tL)- Iodidereagent: f Date analyzed 1 --O2-L..t,, Acid reagent:* ,I T .1 Calibration: I. 1 0 m 1.00 mg/L [Reference standard number -A Note: For samples with a residual chlorine of > 1.0 mnIL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorycontrol standard: Reference standard True value (TV) Measured value (MV) % RS =MV TV x 100 number (mg/L) (m g/L) (acceptable range.= 90 to 110%) la$s353 0.50 o.q(0 .2.o0: Duplicatesample precision: Sample Sample ID J Sample characteristics Residual chlorine '%RFD ((S - D) /l(StD)/2'1) x 100 number . (mg/L) (acceptable range +/- 10%) S Duplicate, D C.)OO ---- Sample meadsurenzents: ,_. Sample Sample ID Sample characteristics Residual chlorine number - __._ _._, _, (mg/L)
;.S.;,,$. Blank (shouldbe= <0.10 mg/L) .0;C- I]
Note,: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV /TV X 100 number (mng/L) (mg/L) (acceptable range 90 to 110%) AtS-, o0o!o~5 05.0 .(Go Reviewed by Date reviewed
Lnvlronmental ' esting ýOlutions, inc. Alkalinity (EPA Method 310.1)
- Mattrix: Water, NDL = 1.0 mg CaCOIL -
Analyst I/pin'r- &ed Date analyzed 1 Titrate samples to pH 4.50 S.U. Titrant normality and multipierdetermination: PH1 of Normalitv Normality (Nv)of HS04 pt Factor or Multiplier Deionized Titrant check Begin End Total = (S ml Na 2CO 3 x 0.05)/E (N x 50000)/ 100 m1 sample water refer.ence standard ml ml ml .0.25/E =Nx 500 4.5 S.U. number number ",ý(E) (acceptabte range 0.018 - 0,022) ! S6(o INRaI N.-rnP C 9,54 ,. , _ o _.Oao-_- _0_.._ LCO1it,? -0 0,0-
- o. OI -D ")Il Laboratory control standard:
Reference True value Sample Alkalinity (MV) ,%RS MV /TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3 L) (acceptable range (rag CaCO,/L) (ml) ml ml ml 90 to 110%) M ýAA3 100 100 lo q -3 -. 10.3 c\L, c .,0 Duplicate sample precision:._..... Sample Alkalinity "/ RPDD Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3 /L) {(S -'D)/[(S+D)/2l} x 100 number (ml). ml ml ml (acceptable range = 10%) __- _____, _________-_,._ IDo 2-.s , 9.105 . 1o .3 s _ 6q _ _ Duplicate 3 D Sample measurements: Sample volume Begin End . Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) 11 .20o'v f tS l -O j.-.3L 3"o q3-3 .., _*__O,__._5 _ q 3.3 '-4o al 1,
-I
ýo. 1'
- 3Sqto 1E -.0 I6o~aS6 ,
'ý5jg..+
8 ~ta ,b 1 5. 1.j5 . 1.-,4t~ N I* N 3, 1.-: .... 1 1 1 ) .. . . 3. . . . . . . . .. . Reviewed by: Date reviewed: I -21IQ'""
ItfbUll OVIU-Q.-' -.- - . Alkalinitv (EPA Method 310.1) Matrix Water, MDL = 1.0 mg CaCOiL 105 3
*Analyst .
Date analyzed Z-"- Titrate samples to pH = 4.50 S.U. Titrant normality and multiIplier determination: pH of Normality Normality-(7v) of H2 S0 4 pH.Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na'CO 3 x 0.05)iE (NVx 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E 4 N x 500 4.5 S&U. number number .(E) (acceptable range = 0018 - 0.022) b, N - A C-rno ,o 6, . C-*0 -m 0.C 0 -_.____.___, l11..._ _ _ _ _ _ _ __._ ____- _-o_- 1-o-
/__
Laboratory conra standard:
. Reference True value Sample Alkalinity(MV) % RS =MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (magCaCO 3/L) (acceptable range (mgCaCO~jL) (ml1) ml ml .ml =90 to IIa0%)
Ir5sqo'g. 100 100 1n. aj3 16. Z "3 D.uplicate satmple precision: Sample Alkalinity %R/PD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3 /L) ((S - D) /[(S+D)/21} x 100 0 number (ml) ml ml ml (acceptable range = 1 %) 12.Oz 0 a}LO lh-A.A 14z2 Io Al.5 "0 .o , 1.2 S 51- .1-.. .. Duplicate To 3q[ D § Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (S.V) volume .Begin End Total Multiplier (Mg CaCO 3/L) (mg CaCOi/L) (ml) ml ml mll
- 50. 10o .?ý.o 3qTo 100*
Sample alkalinity (B) Measured spike value (MV) % R = MV SV x 100 (magCaCOj/L) NV = A - B j(acceptable ,"2nge
. . (mg CaCO3/L) =75 to 125%)..
Sample measur.ements: -, Sample volume. Begin End Total Alkalinity Sample number Sample ID ( ml) ml ml mi Multiplier (mg CaCO3fL)
- 2.o-ou (3 im4-t 1o \o,5 3To Nze6 b.* io.2 .*
12.p2.&.__,_____ q2,e8 I -k1 . 6. !L-3o Ss., 4- , 00,. 3. o 3,0 3 I2.O* <, , .5fl-*. 3w_33 ). .' __.__pP"6 I?.02-.j e ., Bi.4 %.j .~ _______ p tl~;ol -- ri* I'Vc vu*4i ..... , 2a.o MI. [3. __ o l- . 54.5[ W2) 2 q3 3.e ' o- ,:z-,ot -3 ,, 33.o 38.1 c4.3 J, _ _ _ _ 2S._0_7__ __ __ __ L6UL0a 36 2- - - __ _5.1 Reviewved by: Date reviewed: ii.- 6-.OF
. ztivironmeniiai i csýtinz;c o~u-v Total Hardness (EPA Method 130.2) Matrix. Water, MDL 1.0 mg CaCO3 /L Analyst. F77777 _.. ' Timre initiated: Date analyzed Time completed: Titrant normality and multiplier determination: Titrant Normality check. Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range = 0.018 - 0.022) = V x 1000 Laborator control standard: Reference standard True value Sample Hardness (MV) % RS = IV /TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCO/L) (acceptable range (Ing CaCO 3 L) (ml) ml ml ml 90 to 110%)
),,5L5, . 40 50 ,. ji.g .o Diiplicatesam ple recision:
Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier. (mg CaCOJL) ((S - D) /[(S+D)/21} x 100 number " _(ml) ml ml ml (acceptable range = 10%) H-.zo-O', Mrt-s 4,-O _ _. la1c .2 .3 .I ____.2_q Duplicate (B)- U _2 aoC e . q D C) MatrLx spike recovery: q Reference standard Spike value Sample Spike"hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mgCaCO!L) (ml) ml ml ml I .hi - la-V ýo GO aao ., A'4 I i* *-q.o Sample hardness (B) Measured spike value (MV) % R= MV / SV x 100 (mg CaCO 3/L) MV = A - B . (acceptable range (nig CaCO3 /L) 75 to 125%)
*z J,* 5 *,'/
S-ample measurements: __* Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3fL) Blank IV"'-:S C) (should be 0 mg CaCO 3/L)0.0 00 0,0 l*al.*,~o..,, t5,.*H . . -t.5 5.I 2.- ,, q
- 4. Z Note: If >Il 5m] of (itant is used, samnple must be diluted. Reviewed by:Daeeiee Date reviewe'd 1%- c' 0(ýa -
Total Hardness (EPA Method 130.2) Matrix: Water, NIDL = 1.0 mg CaCO 3,L Analyst., 'f- Time initiated: 09%t( Date analyzed -o " Time completed: 0 q 2-1 Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E (Nx 50000)/ 50 ml sample number number (E) (acceptable range = 0.0 18 - 0.022) . NX 1000
,p~q ~ ~o I**to.o a.5 * . 0.0 b oq Laboratory controlstandard:"
Reference standard True value Sample Hardness (MV) ./o RS =MV/ TV x 100 number (TV) volume Begin End Total Multiplier (Mg CaCOs3L) (acceptable range (mgCaCO3fL) (ml) ml ml ml =90 to 110%) 40 50 1*. 2-0 ! 'o4 I . 1o2.5 Duplicatesam le precision. Sample Hardness %RPD= Sample Sample ID volume Begin End Total NMultiplier (mg CaCO3!L) ((S - D) /(S-+D)/2(( x 100 number "(m_ ) ml ml ml (acceptable range= +/-10%)
. oDuphisat - ,(B) . D qo Matrbc spike recoverv: ..
Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOJL) (mg CaCO3 /L) (ml) ml ml ml
.3 .- 2,2Ao.3 Sample hardness (B) Measured spike value (MV) %R MN'IV ISV x 100 (nig CaCO 3 L) MV =.A - B (acceptable range co (mlg CaCOs/L) 1oIW'oO.
= 75 to 125%)
Sample measurements: Sample volume Begin End Total . - Hardness Sample number Sample ID " ml) m Ml ml Multiplier (mg CaCO3/L) Blank
" ,..3 C (shouldbbe 0 mg CaC03lL) - 0-O 0-0 0.0 t2 .oZ.(,riHs H - .o 4.0 cZ
'jOZ ____ 3)i- P LI of-%NuPp" WLU-ip I JYR 36),q '4-4§~
.otizW.0k 380.0 47,0 43 CIO o . o . 2 _ 3.e e.o q.z .
__,___IV _ .. o II0 . Note: If >15ml of titrant-is usedsample must be diluted. Reviewed by: Date reviewed IA
Lnx'ircininental 'L'esting *sowtulofl,1 Total Hardness (EPA Method 130.2) Matrix: Water, MDL 1.0 mg CaCO.L Analyst r - Time initiated: Time. completed: Date analyzed (-Z
r~ant normality and multitjier determination:
Titrant N"7or~m-arjFy-,fh~ek--- .R End Tota___l 'Normality (A) of EDTA pH Factor 'or Multiplier reference "standard ml ml - ml .-------- 4./I -- - (N x 50000)/l?&0O ml sample number number (E) (acceptable ran~ge =0.018 -.0 *.022) 10"6Px'O Laboratory control standard.: Reference standard True value Sample Hardness (MV) % RS MV/TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml = 90 to 110%) 40 50 1301 0-(4.o- (oz. Duplicate sam ple precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCOJL) ((S - D) /I(S+D)/21) x 100 number (ml) ml . " ml ml (acceptable range = 10%o) O It -. ,,S-N,,T t7.,O "- L3. Lq.6 j 3.9 lo, q Duplicate (B) . D, MatrLc spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mag CaCOj/L) (ml) ml ml ml LALA (ý 5~ ) ý5 eA-3, 6( I. Sample hardness (B) Measured spike value (MjV) % R MV I/SV x 100 (Mrg CaCO 3fL) MV = A - B (acceptable range (mg CaCO3IL) =.75 to 125%) g0o 0 loot I. Sample measurements: Sample volume Begin End Total Hardness Sample number SampleID (ml ml ml ml Multiplier (nmg CaCO 3fL) BlIank________ ___ __ (should be 0 mg CaCO3/L) ow0 t,,zci,,O_k A* l:T 6-b ý .6A 4. ______ o06137,0t. 2.* -*.4 _q. 3_0, 3.' -0
-of. % 6z_ C_ a-- j_
Note: If >l 5ml of titrant isusecd,sample must be diluted. Reviewed by: ii Date D reviewed I , II 3 -61.
Alkalinity (EPA Method 310.1) Matrix. Water, MDL = 1.0 mg CaCO/'L Date analyzed Ljý-3 .p6-ý Titrate samp'les to pH = 4:50 S.U. Titrant normality and multiplier determination: Normality (I) of H,SO, pH Factor or Multiplier'
= (5 all NaCO3 x 0.05)/E (:Vx 50000)/ 100 ml sample
-- e--- b. e r 0.25 /E x 500 cceptable r '* "= . O 22..).
-4 Duplicatesam ple precision:
Sample 0.0 o .5 i. Alkalinity %RPD = Sample Sample ID volume. Begin End Total Multiplier (mg CaCO3IL) ((S - D) /[(S+D)/21} x 160 number :.L/A (ml) mlI ml ml (acceptable range +/- 10%)
*,,2Tho \ ,-qJ ID' I iob 3a. *a 4~-. ~-,10.2 s~Qo * '.. . . "7..
Duplicate "
- , D Matrix spike recovery:
Reference Spike value Sample " . Spike alkalinity (A) standard number (SV) volume Begin End: Total . Multiplier (mg CaCO3 /L) (ing CaCO3IL) (ml) -.. ml ml ml. Idsq.-r- bZ Jo te.S- 9.6 11.3- io.z .2 Sample alkalinity (B) . Measured spike value (NIV) %R
% MV SV x100 (mg CaCOIL) MIV =.A - B (acceptable range (ag CaCO3/L) 75 to 125%)
Sam*le measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg. CaCOL) c\. lT"A-* Ca 1lOt 2- 1N0DO4.3 Lo.5 IO.Z . cskoAZL-. 02 Ql, ,J 3.~A 3(0 ŽL ( 9L, 3z ?-~" q3. LL____ (" 35S26o'z I n,13 b:ýue . .. 3o.0 5q j,.7 - - lo*_3 0 q_1 3(. J I. Reviewed by: Date reviewed: \2.-O"-O(,
SeqUoyah Nuclear Plant Biomonitoring November 28 - December 5, 2006 Appendix D Reference Toxicant Test and Control Chart
Pimephalesprom elas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water. 0.9 .I I I C nI i i i +/- I I I I I I USEPA Control Limits (+/- 2 StandardDeviations) 0.8 - 0.7 k 0.6 F-0.5 I F I I I F I F - F I I F F F F I I F 19~ U WarningI I I I ( and I I I I I i I USEPA4 Warning and Control Limits (75t and 901h Percentile CVs) 1.0 z. 0.8 Q43 0.6. 0.4 F I F F F I F F F F F I F I F I F 0.9 I l I I " I I . Laboratory Warning and Control Limits (10th and 25th Percentile CVs) 0.8 0.7 0.6 0.5 Aa Vi I
% ~~ ~ ~ ~
- 3 '00 1
S ' AzSý 3-. S AZ Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chl6ride that would cause a 25% reduction in Pimephales growth for the test population. - - Central Tendency (mean IC 2 5) Warning Limits (mean IC25 + SA.10 or SA.7S) Control Limits (mean IC 25 +/- SX25, SA.90, or 2 Standard Deviatioris)
Environmental Testing Solutions, Inc. PimrepI alesprom elas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Waler State and USEPA L -Laboratory Lab orat on, USEPA USEPA Test number Test date 7-day IC,, CT S Control Limits S,_ I ,Varnting Limits S125 Control Limits STs Warning Limits Sý90 Control Limits (gL KCI) (gIL KCI) CT- 2S CT+2S CT - S,,, CT + S.o CT - Sv,. CT + S,.,, CT -S_,, CT + S, CT - S,,, CT + S,, 1 02-07-06 0.67 2 03-07-06 0.61 0.64 0.04 0.56 0.72 0.08 0.57 0.72 0.13 0.51 0.78 0,24 0.40 0.89 0.93 0.29 0.35 .0.0 3 03-14-06 0.53 0.73 0.23 0.61 0.07 0.47 0.74 0.07 0.53 0.68 0.13 0.48 0.38 0.84 0.27 0.33 0.88 0.1 4 04-04-06 0.57 0.60 0.06 0.48 0.72 0.07 0.52 0.67 0.13 0.47 0.72 0.23" 0.37 0.82 0.27 0.33 0.86 0.1 5 04-18-06 0.64 0.61 0.06 0.49 0.72 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.84. " 0.27 0.33 0.88 0.0 6 04-18-06 0.61 0.61 0.05 .0.51 0.71 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.84 0.27 0.33 0.88 0.0 7 05-09-06 0.57 0.60 0.05 0.51 0,70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.0 8 06-06-06 0.45 0.58 0.07 0.44 0.72 . 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.1 9 06-13-06 0.56 0.58 0.07 .0.45 '..0.71 .007 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.1 10 07-11-06 0.61 0.58 0.06 0.46 0.71 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.80 0.26 0.32 0.85 0.1 11 07-18-06 0.61 0.58 0.06 0.47 0.70 0.07 0.51 0.65 0.12 0.46 0.71 .0.22 0.36 0.81 0.26 0.32 0.85 0.1 12 08-01-06" 0.64 - ,0.59 0.06 0.47 0.71 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.81 0.27- 0.32 0.86 0.1 13 08-08-06 0.67 0.60 0.06 0.47 0.72 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1, 14 08-15-06 0.49 0.59 0.06 0.46 0.72 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.1 15 08-22-06 0.65 0.59 0.06 046 0.72 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1 16 09-19-06 0.61 .0.59 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1 17 10-10-06 0.60 0.59 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1 18 10-17-06 0.64. 0.60 0.06 0.48 0.72 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0:82 0.27 0.33 0.87 0.1 19 11-14-06 .0.54 0.59 0.06 0.47 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1 20 11-28-06 0.63 0.60 0.06 0.48 0.71 0.07 0.67 0.52 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.1 Note: 7-d IC,, = 7-day 25% inhibition concentration, An cstimation oftthe concentration ofpotassiusrs chloride that would cause a 25% reduction in Pintephales growth for the test Population. CT = Central tendency (mean IC25 ). S = Standard deviation of the IC2, values. Laboratory Control and Wantung Limits Laboratory control and warning limnits were established using the standard deviation of the IC2, values corresponding to the 10di and 25th percentile CVs. These ranges are nmorestringent than the control aid warning limits recommended by USEPA for the test tmethod and endpoint. S,.n = Standard deviation corresponding to thle l.0 percentile CV. (S, = 0.12) SAis = Stantdard deviation corresponding to dse 250' percentile CV. (S,,, 0.21) USEPA Control'and Warniiig Limits SA7s = Standard deviation correspotnding to the 75t percentile CV. ( S,.j = 0.38) SAo = Standard deviation correspondbtg to the 9,0 percentile CV. (S,.o = 0.45) CV = Coefficierst of variation of the IC 2, values. US2PA. 2000. Understaurding and Accounting for Method Variability toWhole Effluent Toxicity ApplIcations UtJder the National Pollttant Discharge Elhination Propowi. EIPA.)33-R-D-003 US Enviromunental Protection Agency, Cuici*naat.OH 0~g-'i-oI ohoiooedfros Aq-li BfoSyar,-. Zo.It.260 l11ý2806
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pitnephales promelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Control Control Mean Test number Test date Growth CT. Cv CT MSD PMSD CT Survival for Control Growth N for Control (%) (mg/larae) (mg/larvae) (%)" for PMSD (%) Growth CV (%)
.1 02-07-06 100 0.711 7.8 0.09 13.2 2 03-07-06 100 0.774 0.743 8.4 8.1 0.10 13.3 13.2 3 03-14-06 100 0.745 0.743 9.7 8.6 0.14 18.9 15.1 4 04-04-06 100 0.766 0.749 11.5 9.4 0.08 10.7 14.0 5 04-18-06 100 0.621 0.723 9.7 9.4 0.09 13:8 14.0 6 04-18-06 100 0.668 0.714 13.3 10.1 0.10 15.4 14.2 7 05-09-06 100 0.841 0.732 7.5 9.7 0.12 14.4 14.2 8 06-06-06 100 0.783 0.739 5.9 9.2 0.08 9.6 13.7 9 06-13-06 97.5 0.709 0.735 8.2 9.1 0.16 22.0 .14.6 10 07-11-06 100 0,673 0.729 2.5 8.5 0.09 13.1 14. 4 11 07-18-06 97.5 0.623 0.719 17.1 9.3 0.10 16.6 14.6 12 08-01-06 100 0,746 0.722 10.5 9.4 0.13 17.0 14.8 13 08-08-06 100 0.613 0.713 10.4 9.4 0.08 13.6 14.7
-14 08-15-06 100 0.765 0.717 ,8.0 9.3 0.16 20.4 15.1
- 15. 08-22-06 100 0.768 0.720 8.7 9.3 0.12 15.5. 15.2 16 09-19-06 100 0.699 0.719 12.7 9.5 0.12 17.2 15.3 17 10-10-06 100 0.685 0.717 5.1 9.2 0.10 14.0 15.2 18 10-17-06 100 0.781 0.720 7.3 9.1 0.15 18.7 15.4 19 11-14-06 100 0.737 0.721 5.3 8.9 0.10 13.4 15.3 20 11-28-06 100 0.621 0.716 14.0 9.2 0.12 18.7 15.5 Note.- CV = Coefficient of variation for control growth.
On average, the CV for control.growth is 9.2% in Environmental Testing Solutions, Inc. Pimepltales chronic toxicity tests. Lower CV bound determined by USEPA (I 0h percentile) = 3.5%. Upper CV bound determined by USEPA (90 h percentile) ='20% MSD Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pirnephales growth by 15.5% from the, control.
-Lower PMSD bound determined by USEPA (10" percentile) = 9.4%."
Upper PMSD bound determined by. USEPA (90'h percentile) 35%. CT Central Tendancy.(mean Control Growth, CV, or PMSD) The lower and upperlbounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquatic BioSystems, Inc. 11-28-06
PimephalespromnelasControl Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25. I I I I A Ie a .I2 I I I l r
- 1.00 0"*.75
. 0.50 0.25 USEPA Acceptance Criteria (> 0.25 mug per surviving larvae)
- 'J I I I I I I I I I I 0
KI I I I I I I. I i
- l I 'I .*I" I . 1 -
C - 30
~ Kentucky Acceptance Limit (< 30.0%)
c~ ~ L 20 '.- L-.
~
0
.10 ~.J L
~ I- ~
- 0. 0 U.
I I I I 40 30 USEPA Upper PIMSD Bound (90" percentile < 35.0%) 20 ... 20 ............ . '... 10
- I I" I ] IT I d tI I I " I 1 I
~Test date Control Reproduction, Coeffic.ient of Variation (CV), or Percent Minimum Significant.Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- Central Tendency (mean Control.Growth, CV, or PMSD)
Control Limits (mean Control Growth, CV, or PMSD +-2 Standard.Deviations)
I Page I of 5 fnvironmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales prom elas PpKCICR Test Numbet IOrc Dilution preparationinformnation." Cornrnents." KCI CHM number: C k.\ l Stock preparation: 50 g KCI/L Dissolve 50 g KCI in I -L Deionized water Dilution prep (rag/L) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 18 Diluent volume (mL) 994 991 988 985 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information: Test information: Organism age: 7zq.S T- "jt,..v k, ý - tY Randomizing template: Pu"L-e-Date arid times organisms NV-z-.- N0 -*t
- o *¢50 Incubator number and were born between: shelf location:
Organism source: -ArA {p -2i-oL. Artenia lot number: &L7t&4' 0 Transfer bowl infor mation: pH a SU Temperature 'C Total drying time: Z- -
,,. 2- Date / Time in: - 0 \*,S Average transfer 'volume\ Date / Time Out: I.-... IL<
Oven temperature: too .c.. Dailyfeeding and renewal information: Day ' Date Morn ng Afternoon Test initiation, .MHS Analyst' feedin feeding time renewal, or batch used' o II~a,.o*k time
* \ . termination time
_____ k 2 1 V-3 0-b cf71-0I-C, Is-4\ -. 15 2 _ _ 6 )
*,0qzz ls-21 \ t. " - -
6 }2-b.-tl- c* 0'O \'sqc \.~ 1- *"-z,,-a* 5 /-.---, ' .q f) 1 0*-. . :-d-7,ti U7/I o-oA
": - ,- ? :'i.:i .__2ý Control information." Acceptaitwcte, ciac Sumnary of test endpoints.
% Mortality:
- 20% 7-day LC 0 70"2-Average weight per initial larvae: 0. (2 I . NOEC '50 Average weighf per surviving larvae: Q.. 0.25 mg/laivae LOEC t_ ___
ChV .51tq. (. IC25 1 '?" I
r-rivironmentai. i esting,ýioiutions, inc. Patge 2 ot 5 ii.nvironmcntallesting 5olutIOnS, Inc. Pdce 2 or 5 Species: Pimephales promelas PpKCICR Test Number: 100 Sirvival and Growth Data Day Control 300 mg KCUL 450 mg KCV/L A B C - D E F G H I J K L 0 L /- /0 10 /o /o /0 /o /o . / / 1 to 1) 10 /0 /0 /0 /0 /0 /0 /0 /0 /0 2 D 2/0 0 /. /0 0 10 /0 / /C) /C) /o /0 3, C- C I0 /0 /0 /o /) /0 0 O /0 /0 4 1 to 0 IC 10 /0 10 IC0 10 /0 10 10 /O iO /O /0 /0 /O /0 /0 /0 /0 /0 6 S/0 /0 /0 /0 "0 /0 '0 /0 / /0 /0 /0 to /0 '0 /0 /0 /0 /A) 0 /Q /0 /0 /0 A Pan weight (mg)
'Tray color code:: q. r7 8 1 3q5. 1 sq.1o5 9 1 0 1 Analyst: 1 iq-5 1 I3Ab I'S( itt. [ . . ' . 1 . .
B = Pali + Larvae weight (mag) Anlyt * °N:r' .1b al.Ocl*g si)( II*l1.S5 9.*,q !al.ýa A1.os `I.Sý MA~q Analyst: 037 Y X0T ai01.~9q alao al.Ii.sI~9W ~ ~ i Larvae weight (iji) = A - B Sk -'4~ z S30 l- 2 1 Weighl per initial number . ol larvae (mng) ALI -' _ ~ .
= C / Initial number of larvae 4 ". A 4" O" '
Average Percent weight per reduction initial from control O , *2-\ *.,. .; o/i ,i number of (%) .. " ' : I larvae (ing) ________ _ .______...________ ___.__,__ Comment codes: c =clear, d =dead, fg =fungus, k =killed, m =missing, sk =sick, sm =unusually small, lo = unusutally large, d&r - decanted and-returned. W = wounded. Calcul'ations and data teviewved Comments:
Lnvironmental Testing Solutions, Inc. t'age .) 01 D Species: Pimephalespromelas PpKCICR Test Number: J1( Survival and Growth Data Day 600 mg KCUL 750 mg KCIIL 900 ing KC/L N1 N 0 P Q R S T U V W X 0t 0__ A -) / 1o 1o /00 ) /10 /0 /0 16 fiA/0 q to -* 2 aA (9. *{ 2
- 2. C 14 ,0 2A I a.
, ,4 I, A d
-S Ci ,1 5, LA L4 3 %A , A*
6 -1 Ol Aý LA 7 0 7 s'5 t, 3 L A = Pan weight'(ing) Tray color co.de:: Lý ýIa mxl 13 ."/9 1 .3 `1 1 3.3 3 I r. u .M . Analyst: (64-! (m'B g=) Pan + Larvae weight i. r. ao~ss 11.61 i7.0ý AIF. Ito.* 15.S 6-`1 13.5ý t+t- PS-.0 Larvae weight (mg) = A - B L,$ -IA 1.72 *.SS "2.\ , o 0ZA 0-, O 0 ,. W eight per initial iu nlber ILI of larvae (mg) C " o7 "
= C / Initial number of larvae " . A V 0 V o0 0 0 0' 10 Average Percent weight per reduction initial fron con trol . - "1 , .. z.
number of (%) - larvae (11tg) L Comment codes: c =clear, d =deid,; f' tungus, k =killed, m missing, sk sick, sm unusu'llysmall, lg unusually large, d&r = decanted and returned, w.= wounded. Calculations and data reviewed: Comments:
Environmental Testing Solutions, Inc. Chronic Whole Effluent ToxicityTest (EPA-821-R-02-013, Method 1000.0)' Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR H 146 (#106 at 351 Depot St.) Test dates: November 28 - December 05, 2006 ReveiIed by: Conerio, (mo/L RIplnice Initial n-ob-r or Cinol nRnbr of A P.n ,*hih (mI) B - P-n - Lar.-c Laz-a onithi (n. SnZio) IsSurviving Moo,,o.,.itht/ Nunytbtr CoWttnt otoor.oitoo Weight I XONIioUo-MýC tot oýuMorm
-UNJn*1-1oo Moooo Infa Cn ot norloti.JlonP--t odulkl..
tCt5 l-n , o ti t ( ra)n A H no oboroti12,o, (f r) S urolon! n uoborot ( toO* tOi r~Oi~n o o'ln ( ) ('H.) n - b. ofl -t (r/. oon-oi ('/.) A 10 10 14.55 20.37 5.82 0.582 0.502 Control [ 10 10 13.96 20.18 6.22 0.622 . 0.621 140 0622 100.0 0.62t 14.0 Not applicab C 10 10 13.64 21.05 7.41 0.741 0..74 I D 10 10 14.56 19.94 5.38 , 0.538 0.538 _ E 10 10 14.85 21.06 6.2)1 0.621 0.621 300 F 10 10 14.78 '. 2 .51 673. 0.673 0.580 14,9 0673 100.0 0.588 14.9 53 G . 10 10 13.95 19.85 5.90 0.590 0.590 If 10 10 14ý62 19.29 4.67 0.467 0.467 10 to 10 15.05 21.32 6.27 0.627 0.627 450 10 t 10 . 14.49 21.03 - 6.54 0.654 0.609 7.0 0.654 100.0 0.609 7.0 1.9 1K 10 t0 14.01 19.55 5.54 0.554 0.554 L 10 10 14.44 20.46 6.02 0.602 0.602 M 10 7 13.74 10.29 " 4.55 0.650 0.455 N 10 9 14.37 18.84 4.47 0.497 0.654 27.0 0.447 80.0 0.520 26.0 16.3 t,10 . 8 13.33 20.55 7.22 0.903 0.722 p10 8 15.06 19.61 4.55 0.569. 0.455 10 5 14.11 17.04 2.93 0.586 0.293 750. R 10 4 13.05 16.17 2.32 0.580 0.593 6.4 0.232 40.0 0.238 22.2 61.7 S 10 4 14.28 16.87 2.59 0.648 0.259 T .10 3 14.30 15.98 1.68 0.560 0,168 U "10 0 0.00 0.00 0.00 0.000 0.000 900 V "1. I . 290 13.5 0.68 0.660 0.650 6.5 0.066 7.5 0.048 124.9 92.3 W 10 0 0.00 0.00 0.00" 0.000 0.000 X t0 2 13.77 15.01 1.24 0.620 0.124 Dunnett's MSD value: 0.1163 MSD = Minisliu Significant Difference PMSD: 18.7 PMSD Peicent Minimum Significant Difference PMSD is a nseasirý of test precision. The PMSD is (lte inutuium percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.5% from the control (determined thooug reference toxicant testing). Lower PMSD bound deteimined by USEPA (10th percentile) = 9.4%. Upper PMSD bound determined by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted ftom 19 laboratories for Pitiephales growth in chronic reference toxicant tests. USEPA. 2000. Understanduig and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elht.inatinn Program. EPA-833-R-00-003, US Enviromnental Protection Agency, Cincinnati, oH. Organisms obtlainedfroin Aquatic BioSystems. Itc. 11-28-06
Environmental Testing Solutions, Inc. Statistical Analyses Lirval Fish Growtlh and Survivil Test-7 Day Survival StartDatc: :1128/2006 TestID. PpKCICR Sample ID: REF-Ref Toxicait End Date 12/5/2006 Lab ID: ETS-Enrir Testing Sol. Sample Type: KCL-Potasriunn chloride Sample Date: Protocol. FWCHR-EPA-821-R-02-013 Test Species: PP-Ponephales promneas Comments:' Cunc-mg/L 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1,0000 300 1 0000 1.0000 10000 1.0000 450 1.0000 1.00000 1.0000 1.00 600 0.7000 0.9000 03000 0.8000 750 0.5000 0.4000 0.4000 .03000 900 0.0000 0,1000 0.0000 0.2000 Transform: Aresin Square Rool Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean bl Main lanx CV% N Sum Critical R-esp Number D-Control 1.0000 10000 1,4120 1 4120 1.4120 0.000 4 0 40 300 1 0000 1.0000 1.4120 1.4120 14120 0.000 4 18.00 1000 0 40 450 1.0000 1:0000 14120 1.4120 1,4120 0000 4 18.00 1000 0 40 "00 0.8000 0.8000 1.1136 09912 1 2490 9478 4 10.00 1000 8 40 "750 0.4000 0.4000 0.6836 0.5796 0.7854 12.289 4 10.00 1000 24 40 "900 0.0750 00750 0.2757 0.1588 0.4636 53 294 4 1000 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shapizo-Wilk's Test indicates ,on-normal dtsaibutnon (p <= 0.01) 0,79563605S 0884 0 52113979 1,618140669 Equalty of variawce cannot be conrtlned. Hypothesis Test(l-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 450 600 5196152423 Treatmeats as D-Control Maximum LikElihood-Probit Parameter - Value SE 95% Fiducial Limits Control Clsi-Sq Critical P-valae Mu Sigma ltur Stone 13.16756983 1.804761012 9.630238175 16.70490148 0 0.603478868 7.814727783 0.9 2849547769 0.075944158 3 Intercept -32.5216192 5,153545422 -426223685 -22.42067 TSCR - 1.0 Point Prabil. mg/L 95% Fiducial Limits 0.9 ECOI 2.674 470.8420465 400,1998345 519.0476467 EC05 3.355 530.433257 2 469.5403967 571.9700907 0.8 ECiO 3.710 565.2250698 510.7644752 602.9745341 0,7 ECI5 3.964 539.9792149 540 2163463 625.2854703 EC20 4.158 610.4239368 564,4820016 6-44.0018976 006 EC25 4326 628.5273978 585.815428 660.900742 r0.5 EC40 4.747 676.5619012 640 8299499 708 1026987 ECSO 5.000 707 2089864 673,905148 740.8218287. *02 EC60 5.253 739244 3377 706.2829966 7776913987 013 EC75 5.674 79537402488 758.3724828 848 8783814 02 EC8O 5.842 819 3396792 - 778.711640 8804631334 EC85 6.036 8477323071 802,4164168 919.5582422 0.1 EC90 6.282 884.8590825 832.4719031 972 1556955 0.0 EC95 . 6.645 942.8981527 877.9592221 1057,071685 10 100 1000 10000 EC99 7.326 1062234314 967.85566S5 1239739429 Dose mgIL Dose-Response Plot a.0a,0 0 0 g g 0 o - DN Orga-'sms obtainedfmm Aquatic BioSystes-, lnc,. 11-28-06
Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 D*y Growth Start Date: 11/28/2006 Test ID: PpKCICR Sample ID: R"EF-RFefToxi/nt End Date: .12/5/2006 Lab ID: ETS-Ervix. Testing Solt Sample Type: KCL-Potaosithon chloride Sample Date: Protocol FWCHR-EPA-821-R-02-01 3 Test Species: PP-Pianephiales promelas Coroonents: Conc-mg/L, 1 2 3 4 D-Conntol 0,5820 0,6220 0.7410 0 5380 300".- 0,6210 06730 05900 04670 450 0.6270 0.6540 05540 06020 600 04550 0.4470 0.7220 04550 750 02930 02320 0 290 0.1680 900 0.0000 00660 0.0000 0.1246 Transform: Untransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Mibn Max CV% N t-Stat Critical MISD Mean N-Mean D-Contrcl 0.6208 1.0000 06208 0.5380 0.7410 14.047 4 0.6208 1.0000 300 0 5878 0.9468 0.5878 0,4670 0,6730 14 884 4 0.619 2.180 0.1163 .. 0.5985 09642. 450 0.6093 0.9815 0.6093 05540 0.6540 6.978 4 0,216 2.180 0.1163 05985 . 0.9642 600 0 5198 0 8373 0 596 0 4470 0.7220 23952 4 0.5198 0.8373 750 0 2380 03034 02380 0 1680 0.2930 22.236 4 02380 0.3834 900 0,0180 00773 0.04,0 0.0000 0 1240 124.907 4 0.0480 0.0773 Auxiliary Tesai Statistic Critical Skes Kart Shap-ro-WL's Test indicates normal dnstiibution (p > 0:01) 0.989200532 0805 -0.0220246 -0.11689876 Bartlett's Test ondicates equal voriances (p 0.48) 1461641073 9.2103405
}lNpotheai Test (1-tail, 0.08) NOEC LOEC ChV TU MSDu MSlOp MSB MSE F-Prob df DtLanetts Test 450 >450 0.11625787 0 187286138 0.001122333 0.005608028 0824389994 2,9 Treatmnents vs D-Contool Linear Interpolation (200 Resamples)
Point mg/L SD 95%/*CL(Exp) Sksw IC05 466 74 175 37 0.00 699.35 - -0.3523 IClO 525.86 120.23 000 633.50 -1.3121 [l5 584.98 7433 115.33 668.60 -1.9295 t0 IC20 612.32 44.55 432.71 67224 -0 8215 0.9 IC23 628 85 34.30 485.90 682.23 -0.8810 IC40 678 42 19.72 60793 716 26 -0.4679 0.* ICSO 711.47 15.17 65793 746.32 . .. 2958 0.7 o0.0 a000 04 0.3 0.2 0.1 0.0 0 200 - 400 600 000 10.00 Doso mg/L Dose-Response Plot 1-tail 0.05 level of signtficance
.*. g to 02 Orgoni-n s obtained/rosa Aquatic Bio,ýy~vtess, 1c71-2-0 11-2S-06
Environmental Testing Solutions, Inc. Page "4of 5 Species: Pinephalespromelas PpKCICR Test Number JOG Daily Chemistry: Day
- 0. 1 2 Analyst Vnt (I_-
- I I____, _Vý _______
Concentration Parameter - CONTROL pH (S.U.) -- 757 T)6 7575 .. 5-DO "(rng/L) . ". ,, 6 '?,"? '1- 3 Conductivity (p mhos/crn) 316__ __ (mg CaCO*/) Hardness . (mg CaCO 3!L-) 'L Temperature (°C) ZqA.L* ZA 2- "7- .SS.S "r .5$.* Z-. pH (S.U.) r94, '.5j ./ '.c- '/,)'.55-300 DO (mg/L). to *AJ '7q _ _ q. _ -- __ mg KCIL Conductivity (p.m hoslc m) *:;" :<':'a;s: Temperature . nA -q
"- Q. q.o.q -q (C ) " 114.
pH (S.CU) "_,__- "..__ _______ _ .__0_"__ ._-_. __ DO (m-/L) D - 9.c _ . 2..9 _ 450 mg KCUL Conductivity 'i .: . (pmhos/cm) i}' O .l. t.., Temperature - - i" . (OC) L
-z2.\ LL 2 _l k pH(S U.) "___. j ,1p t.o .S DO.(mg/L) '7, .0 , C2 600 mg KCUL Conductiviiy
(,u mhos/cm ) q .-;%:*3 .';!: .
;: .:# lb-5O
;i*, :: -; ::;>< : ,'i,":
Temperature. 7..A .0zq . 2q .o 2q G. lLA (0C) pH (S.U.) J Jq ""j. j- '.O-7 .O!2 . .. DO (mg/L) '.6 ,)..4 O " , .- ,' 750 mg KCUL Conductivity " ; .: . ;.; .;' (pmhos/cm) ______.... Temperature ' ",
- pH (S..U.) _ ,54__ __ .__ __ _(*7"_, __ _ 79-___
DO (mg/L) ,'7 %.* . , '1L 900 mg KCL'L Conductivity . . (p mhos/cm) 1q30 _. ____, _ (: b- I 'C.
- 9., -0
___. _ _ Temperature (°C) zq.-1 "A...\ q c .*,.L 7A. q1 STOCK Conductivity .zqo0 29D) , _:__.._ ___ ____- "______... ______________ (pimhos/c mn) ....
-nta i a itiaI i ! IntI i a Initial Final Initial Final Initial Final
Environmental 1 esting .,OiUulons, 111h. Species: Piniephalespromelas PpKCICR Test Numbei': 16p Day_.'__.._.a, 3'4 5 Analyst . V't/-
- i. - I*.'-Ey&.-- V - __ .. ___
Concentration Param eter .. . ... "_... .. ... .. CONTROL pH (S.U.) 9.0 2 J.z q. .2a
)_.2 -
DO(mg/L) 2 ""q 2. 9__- .6p (b*n hos/cm) __ __ __ _ __ __ _ __ __ __ __ __ _ . Alkalinity ~ ~ magCaCOI/L) . Hardness '- Temperature (oC) "__ .___'__ A.__z_. __ ____, - _ ___7 ___ _____. _ ___ _ _ _z_. _. pH (S.U.) 9.{I JriLd- 9.*2 U,: 9j5q " 2 M. L T,3 '-} "g DO (mg/L) - .+ . ._., 300 mg KCIIL Conductivity: "2,,{ (pmhos/cm) 8 2z, _ _ _ ~ ___ T em peratu re T'A ,,-- 114 -'.S. (.0C) " _ _"__ pH (S.U.) 9.99 .f L " u "3g §-q 7
'.30 .- 14 ")-(06 DO (mg/L) "-3 R} 9,-.Z - q.5 450 mg KCUL Conductivity . -. { ,:30 ,*<
(pmhos/cm) 30jC '- Y -- Temperature . 1.,q v,.c "h~q."2 -L "ji . (0C ) "1q_ -2S____ pH (S.U.) . lI 95 J3l 9.'l 9-L ".3L 2 3. 600 mg KC/L DO (mgfL) . '42 "- . -.. Conductivity S ().imhos/cm) __._______ 30________ Temperature 2qq.S -.. q -2Zq.- 2A-(oC) "I___ __ _ _ -_ 1 1._- pH (S.U.). '4.l (3qo1qq. 9/40Q 2.3 T.4q D O (mr g / L ) .,_ _ _ _ ' 48 ' .o 9. 9 -3 " 01 750 mg KCL'L Conductivity - '-V 10% 2z : (umhos/cm) I O0 .. ... C. . Temperature - 2 1 "2.Z W 1 (ýC) .2_ __ pH (S.U.) ,- q. (, . '~Y* % 2". L L9., DO (mg/L) '. . . 2,Z 1-f. 94 -4. 900 mg KCUL Conductivity .:- ;. : Temperature 2 . -7 2( . -q S ,zq * ", S 2q S(Tc ) (Prmhos/cm) "{i!{!;(!@ "}*** 0 *" " =... Initial Final IInitial 11 Final Initial Final Initial Final Initial I Final Initial Final Initial Final Initial Final
Ceriodaphnia- dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 USA Control I 2 S De iv io rv USEPA Control Limits (1 2 StandardDeviations) 1.10 V 1.08 1.06 1.04 I I -.I I I I I I I I I I I . I i I I I I I 2 5 I I I I I I I I I I I I I I I I II I I I I I U. USEPA Warning and Control Limits (75th and 90 Percentile C VS) 2.0 1.5 LFI U 1.0 0.5 I~ II . ... ... .. ..... 1.4 ] I I I I I ] I ] I I I "1 I I I I I 1 I Laboratory Warning and Control Limits (10th and 25 h PercentileCVs) 1.3
.1.2 1.1 1.0 0.9 0.8 Z16 Zb 1)ý, Zfa ZbZ'Vz1- ý)16 Z6, Z'ýZV %1Y :j, ,Z6 IS,-rýb Z-b 1), ýý)b z 6,zb N I)b
%I, . Z1, z'-' 03- Z, - N, Al-Z1 AI, Z01) Z9 %I) %N Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- Central Tendency (mean ICs2 5) Warning Limits (mean IC25 +/- S A.O or SA.7s) ControlLimits (mean IC22 s SA.25, SA.90, or 2 Standard Deviations)
Environmental Testing Solutions, Inc. Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC,, CT S Control Linits SA.1 0 Warning Limits Control Limits S.,.9 SA.25 S A-7s Wanting Linits Control Linits (g/LNaCI) (g/LNaCI) CT-2S CT+2S CT - S,0 CT + St CT - S,,s CT + SA,,5 CT - Skis CT + SM5 CT - Sa, CT 4- S,,, 1 06-07-05 1.05 2 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 :'0.66 0.40 1.71
.3 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14. 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 4 08-09-05 1.08 1.06 0.01 1.04 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 5 09-13-05 1.04 :106 0.01 1.03 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 6 10-04-05 1.09. 1.06 "0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 7 11:01:05 1.08 1.06 0:02 1.03 1.10 0.09 0.98 1.15 0.18 .0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 8 12-06-05 1:04 1.06 0.02 1.03 1.10 0.08 ý0.98 1.15 0.18 0.88 1.24 0.48 0.58 .1.54 0.40 1.72 0.66 9 01-10-06 -1.08 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 10 02-07-06 1.07 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.40 1.72 0.66 11 02-07-06 1.06 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0+58 1.54 0.40 1.72 0.66 12 03-07-06 1.05 1.06 0.02 1.03 1.09 0.08 0.98 1.15 0.18 0.8M 1.24 0.48 0.58 1.54 0.66 0.40 1.72 13 .04-04-06 1.06 1.06 0.01 1.03 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.46 1.72 14 05-02-06 1.08 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.58 0,48 1.54 0.66 0.40 1.72 15 06-06-06 1.06 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.58 0.48 1,54 0.66 0:40 1.72 16 07-11-06 1.09 1.06 0.02 1.03 i'.09' 0.09 0.98 1.15 0.18 0.88 1.25 0,48 0.59 1.54 0.66 0.40 1.72 17 08-08-06 1.07 1.06 0:01 1.04 '1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 18 09-12-06 1:06 1.06 0.01 1.04 1.09 0.69 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 19 10-03-06 .1.06 1.06 0.01 1.04 1.09 0.09 0+98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 6.66 0.40 1.72 20 11-07-06 1.06 1.06 0.01 1.04 1.09 0:09 0.98 1.15 0.18 0.818 1.25 0.48 0.59 1.54 0.66 0.40 1.72 Note. 7-d IC 25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia r"production for the test population.
CT = Central tendency (nsean IC25 ). S = Standard deviation ofthe-IC2n values. Laboratory Control antd Wanting Lintits
- Laboratory control and warning Iintits were established using the standard deviation of the IC values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and wamin 21 linmits recommended by USEPA for the test method and endpoint.
S&10. = Standard deviation corresponding to the 10h percentile CV. (SA ,0 = 0.08) SA.s = Standard deviation corresponding to the 25a percentile CV. (Si27 = 0.17) USEPA Contrul and Waning Linits SA7s = Standard deviation corresponding to the 75d percenltile CV. (SA17= 0.45) S,,a = Standaid deviation Corresponding to the 9W, percentile CV. (SA9a = 0.62) CV= Coeffcient of variation of the 1C2 5 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Appiications'Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmeneal Protection Agency, Cincinnati, 10-03-06
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphniadubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test numser Control Control Mean Testdafe CT CV . CT ' MSD PMSD CT number Survival Reproduction for Control Mean for Control (%) (offispring/femal!) Reproduction (%) Reproduction (%) for PMSD (%) (offspring/female) Cv (%) 1 06-07-05 100 30.4 5.0 2.6 8.5 2 07-12-05 100 30.4 30.4 7.5 6.2 2.7 8.8 8.7 3 07-12-05 100 31.1 '30.6 7.2 6.5 3.2 10.2 9.2 4 08-09-05 100 28.3 30.1 7.3 6.7 2.9 10.3 9.5 5 09-13-05 100 27.9 29.6 7.3 6.8 3.9 13.9 10.3 6 10-04-05 100 27.0 29.2 5.8 6.7 3.0 11.1 10.5 7 11-01-05 100 28.4 29.1 10.3 7.2 3.8 13.2 10.9 8 12-06-05 100 32.6 29.5 .6.3 7.1 2.3 7.1 10.4 9- 01-10-06 100 29.2 29.5 4.8 6.8 2.3 7.8 10.1 10 02-07-06 30.7 29.6 6.0 6.7 2.4 7.9 9.9 100 11 02-07-06 29.9 29.6 6.8 6.7 2.2 7.5 9.7 100 12 03 06. 28.8 29.6 5.9 6.7 2.6 8.9 9.6 100 13 04-04-06 27.0 29.4: 5.2 6.5 2.1 7.6 9.5 100 14 05-02-06 28,6 29.3 8.6 6.7 2.7 9.3 9.4 100 100. 15 06-06-06 30.3 29.4 5.2 6.6 3.0 9.8 9.5 100 16 07-11-06 29.0 29.4 5.4 6.5 2.5 8.6 9.4 100 17 08-08-06 28.6 29.3 8.9 6.7 3.7 12.8 9.6 100 18 09-12-06 30.9 29.4 5.4 6.6 3.3 10.6 9.7 100 19 10-03-06 32.3 29.5 4. 6 6.5 3.1 9.5 9.7 100 20 11-07-06 31.0. 29 6 6.3 6.5 2.4 7.8 9.6 Note: CV.= Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.5%/ in Environmental Testing Solutions, Inc. Ceriodaph'niachronic toxicity tests. Lower CV bound deterrnined by USEPA (10" percentile) = 8.9%. "
-Upper CV bound determined by USEPA ( 9 0 thpercentile) 42%
MSD = Minimum Significant. Difference PMSD = Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum"percent difference betveen the control and treatment that can be declared statistically significant in a whole effluest toxicity.test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Cernodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA ( 1 0 ' percentile)" 11%. Upper PMSD bound determined by USEPA (90'h percentile) 37%. CT= Central Tendancy (Mean Control Reproduction, CV, or PMSD) . The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic refierence toxicant tests. U.SEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, 011. 11-07-06
Ceriodaphniadubia Control Reproduction, Coefficient of Variation, and.PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 0e 35
-~~~~~. . . ... .
0, 30 E 25 0- 20 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female) F I i I F ! . F F I I F C-C- I 1 t I* I I I I I I I F I I F I I
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08 40 North Carolina Acceptance Limit (< 40.0%) 0 30 Kentucky Acceptance Limit (< 30.0%) 0..
'U 0-20 0o ",' 10. . . . . ..
I I I ......I....I I
- I t I F f 40 I I I I I I II I I I I I I I I I I I USEPA Upper PMSD Bound ( 9 .01h percen'tile < 37.0%)
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Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the con trol and treatment that can be declared statistically significant, Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
SodiumChloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniaduhia CdNaCLCR #: . Dilufion preparationinformation. Comments: NaCI CHM number: C k.A" I.-L o Stock preparation,! 100 g NaCM/ (dissolve 50 g NaCl in500 ml
.deionized water)
Dilution prep (mWL) 600 800 1000 1200 1400 Stock volume.(mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism information: Test information. Organism age: < 24-hours old Randomizing template: CAA W._ Date and times organisms ,*,- -rO "ZOo'7
%S4--o- Incubator number and were born between: shelf location: __"__
Organism source: Io03\-o6,A U FS ,Z,3.4.:*St, , , YCT batch: _-_-__, Transfer bowl information: pH =-..3 SU Temperature = CC Selenastrum batch: m.'28-o. Daily renewal information: Day Date Test initiation, MHS water " Analyst renewal, or batch used termination time 0 l-cr-GOt 112"1 10-2-0q- is ' 1 k-IC)-t 6 1050 10- ri-ct, IS 2 0o11-0Q, I10 ,'in C)- o C" 4 II. -1206 _. 0 * "., C. 6 [. 12-0-6 Io. 210-L 0a,. C 7 Controlinformation: Acceptance criteria Sunmary of test endpoints. % of Mate Adults: 07, . <20% 7-day LCS > iqoo % Adults having 31' Broods.: " c7. .> 80% NOEC 000____ % Mortality: 7, .
- 20% LOEC _LO_0 Mean Offspring/Female: 1'.0 2-15.0 offspring/female ChV fcI0qs.'. -.
% CV: .. I < 40.01% IC25 I c tL: 0
1[,lIVIl Ull11rllaltll I C,-LlUV 001U~l1011N 111C. -;' ý V, U Species: Ceriodaphniadubia CdNaCLCR 1: *S CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I 'Young produced (j 0 'Co C Adult mortality 2 Young produced _____ c____ ___ 0 ___ 0___ __ Adultm rai _ I " -I L ,- ___ I Adult mortality , *.f k.h.._K _ .. 1 _ 3 You ng produced ________ cj __ c___ Adult mortality ___ _ . _ _ . ___ i " _ " [ * '-A LA+
- j O] " _
Toal yo ung produced ods produced Young ) Co 0* 0 0" Adult mortality, L=D_=_dead) [ 6 Young produced 0tio FiaAd ult m~ortality' t.. ...
- k.._ k, ._
- k,_. .z_ , k_ .
0 Young produced k s Sk r aS e ion Data La Total young produced i Z9 ( - z. 5 z 0-2-. FinflAdult Mortality Adl - ,. ._ - *
%mMortatlity*',
. _ . v .
X for 3'd Broods 5 .2ý. ' " Note: Adult mortality (L ive, D dead)I ___I ______ 1__ -- ___1__.__ Con cen tration:]
% Mortality: 07, Mean Offs rut /emale: 31.0J 600 mg.NaCUfL Survival and Reproduction Data
____I Replicate number. Day___ ce 1 2 3 4 5 6 ý'7 8 9 110 Dayprd produced Adult mortality 00Youngl . cG, c_ c_0 3oa Young produced c, C) c) 0 Adult mortality_ ___._ [='N. 7 JL __.__ _..__ _ _ __.__ _.... ._. LA Young produced
*lo ,o*.
3 _A Adult mortality 24 onprduced Young produced tCle ,- ___ 5 , ,_ s , __ E ___ ,-__ 6 Youing produced C) 1L1 'GI Ia o o 6IAdult mortalirty .'J -'~ 7 Young produced l)lA I i jt 1i Total young produced 32 -2L ZR 11 B Is 3 2\ 30 Final Adult Mortality ~1 -.-- '. Note: Adult mortality (L = live, D = dead) Concentration:
% Mortality: 0111 Mean Offspring/Female: 31.1
% Reduction from Control: - 2.-57.
I2~uI VIZ UtAiflIV.111a1 A. t2L1AA~, .J'JXtAtfl~JhJJ~ *AA~. Species: Ceriodaphniadubia CdNaCLCR 4: 5-5 f 800 m- NaCUL Survival and Reproduction Data J _Replicate number Day "._ _ 2 3 4 5 6 7 8 9 10 l Young produced 0 c)j Q ] C5 0 c)*0 0 "( Adult mortality p Q .. . ___ _ _, ,, .. __., 2 Young produced 0 C_ 0 :0 = _D Adult mortality I - I--V '-- \- I ý-- I Adult mortality Young produced Y f) 01 ii 1 2Fe i C) I, j ~ c I c J f
*-on* I oI,.o,,* ,, 'i-i-I i ' -. I, i i.- I. _"
67 .--] Adult mortality Young produced . 1 I5 c i
- Toal young produced 32. 12 : 3 3,0 I C7. ' 2 0o
*Final Adult Nfortality . _. L. L.
. . L. ,
Note: Adult mortality (L live, D dead) Concentration:
%Mortality: O" Mean Offspring/Female: 3 1.o
% Reduction from Control: C) ,,.
1000 mg NaCUfL Siurvival and Reproduction Data Replicate number Day _ _0 1 2 _3 5 6 7 8 9 I Young produced C) 0 0__ _______ C C_ G 2 I Adult mortality Yýoung producoed o 3 2Adult mortality oung produced 0)
*..i ,
C- J~ [ __ * (jc c)_J~ n.. 4 [. Young produced ' I (A l L ] LO '-A--q '* " 1..111 I Adult mortality Young produced _ _ k._._ K., I U , - .. 1 o 1+1_[ Ifl iV_7F i[
-F1J 6
Adult mortality t[ I 7 Young produced ILA 1I,, IS I &5 iS 133q i' Total young produced 3 2AZ1 Z -9 - k 1," - 31 Z'9 3 Z 2-2. Final Adult Mortality . . j . L ._ - L _ Note: Adult mortality (L live, D = dead) Concentration:
% Mortality: 07, Mean Offspring/Female: 3o-.
% Reduction from Control: ',o
Species: Ceriodaphniadubia CdNaCLCR#: 5 1200 mg NaCIiL Survival and Reproduction Data _.Replicate number Day 1 32.3 4 5 6 7 8 9 1 10 I Young produced '0O 0 0 0 0 0* 0 0 )I Adult mortality . -_ Young produced I =[ 1[ lo 1 0 c] I o CI Adultmortality %[ '.- I i
- l,_
3 Young produced _--_ 0 0--1 . L_ aL) - Adult mortality
- _ _ I, . .. .- _ I ._
Young produced
.dlmortality lI 0._,'i O-Q["
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"-" '.i
*" % L*[-+/-" I Adult mortality CD___ 0___ 0___ 1___ 2-___ Co_0__
C____ Young produced Adult mortality 7 Young.produced.., Q© f O0 *o 0 Totalyoung produced i I LA 0 4. Final Adult'for~tality ' * .. -:1-1 ._ - , .
- Note: Adult.mortality. (L ligel D dead)
Concentration:
% Mortality: 07.
Mean Offspring/Female: q I
% Reduction from Control: -1o.
1400 mg NaCIIL Survival and Reproduction Data
- Replicate number' Day 1 Young produced 0 O2 C3 ) C4 5 6 7
© 8 C) 9 C)10 Day Adut . 1ralt i6_
2 _ 2 Young produced 0___R_ 0 n 1* 0o 1 C) 10 I Adult mortality "__ __ * ' IuI L . L_
*70Lo 3
4 Ault motaot Young produced Young produced g L cL cLL) L -O - Adult mortality.__._.. 6 *Young produced 0 Adult mortali___ ,_. . L_3 _ _ 7 Youngproduced 0 0 hC CK)7.C]o ,,,
- Io Total young produced 0- 0 O ) olo "
Final Adult M ortality [ t ' " t_ L ... j '. Note: Adult mortality (L live, D dead) Concentration: ,
% Mortality: zlerL Mean Offspring/Female:
% Reduction from C6ntrol: 91.,.1
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Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphn ia Survival and Reproduction. Test-Reproduction Start Date: 1117/2006 Test MD: CdNaCICR Sample ID: REF RefToxicant End Date: 11114/2006 Lab ID: ETS-Eno'. Testing Sol. Sample Type: NACL-Sodiam chloride Sample Date: Protocol. FWCH-R-EPA-82 I-R-02-013 Test Species: CD-Cenodaphsia d,.ba Comments: Conc-mg/L ,1 2 3 4 5 6 7 8 9 10 D-Conarol 32000 33000 30,000 31.000 29.000 34000 32.000 32000 28000 29000 600 32000 34.000 29,000 31000 35.000 33.000 30.000 31.000 32.000 30.000 800 32.000 29.000 33.000 32.000 30.000 31 000 32.000 30.000 33000 28.000 1000 34 000 27.000 29.000 29.000 31.000 33.000 31.000 29.000 32.000 28.000 1200 13000 8.000 10.000 80000 12 000 16.000 4.000 6000 8.000 6.000 1400 7.000 3.000 0.000 0.000 1.000 4.000 3.000 2,000 4.000 1.000 Tronslform: Untransformed 1-Tailed Isotonic Cone-mg/L Miean N-Mean Mean Min May CV% N t-Stat . Critical MSD Mean N-Mean D-Control 31.000 1.0000 31.000 28000 34.000 6,270 10 31.350 1.0000 600 31 700 1.0226 31.700 29000 35.000 2958 10 -0662 2.287 2,417 31.350 1.0000 800 31 000 1.0000 31.000 28 000 , 33.000 5.483 10 .0000 2 287 2.417 31 000 0.9888 1000 30.300 0.9774 30?300 27.000 34.000 7.469 10 0.662 2287 2.417 30300 0.9665 11200 9.100 0.2935 9.100 4.000 16.000 40276 10 20.721 2287 2.417 9.100 02903
- 1400 2.500 0.0806 2.500 0.000 7.000 86.923 10 26.966 2.287 2.417 2 500 0.0797 AUriliarty Test3 Statistli Critical Skeos YKurt Kolmogorov D Test indicates normal dashnbution (p > 0, 1) 0.81748134 1 035 0.44619717 0.3712335 Bartlett's Test indicates equal variances (p o0,18) 7.55277777 1530862722 Hypothesis Test (I-tail, 0,05) NOEC LOEC ChV TU" MSDu MSDp OISB MSE F-Prob df Dwmaett's Test . 1000 1200 1095 44512 2 41677734 007796056 1738.96 5.58518519 16E-38 5, 5"1 Treaonents osD-Control Linear Interpolation (200 Resamples)
Point mg/L SD 95% CL Skew IC03 1004.88208 51.0030904 825.635417 1014.14902 -2.5889 ICI0 1019.66981 596832498 1007.34438 1028.34195 -0.3117 IC15 1034.45755 5,61835965 1023,38686 1043.09093 -0.2720 1.0 IC20 1049:24528 5.40692453 1038,23354 1057.91483 -0 2188 0.9 IC25 1064.03302 5,35046793 1052 85837 1072.17403 -0.1422 IC40 1108.39623 '6.09494531 1093559306 1118.42537 0.20533 IC50 1137,9717 7 17272292 1123,85682 1151,46069 0.4115 0.7 06 0.5 02 0,4 0.3 0.2 0.1 0 500 1000 1500 Dose mg/L Dose-Response Plot 1-1ai4*0.05level of sig nificance
,*25 u
o 2 20 10 11-07-06
Elivil Species: Ceriodaphniadubia CdNaCLCR 4: _ _ Dail Chemistry: i 0 Day 2 C netain Parameter-----*] "R *V**- A']* ,-**t2 DO (ma9. L 9. .9a.( CONTROL Conductivity (Pmrhos/cm) 12 Alkalinity (mg CaCO 3/L) Hardness (mg CaCO3 L)
- Temperature -- L 4 7s 0 . t. O v_ ,-..
( C) 600 mg NaCVlL Conductivity ID (pmhos/cm) /Y/6 ,. Temperature !
- t 'L_
* ~ PH (S.U.)
~~DO (mg/L) ** .qq 9-q("
*q
- Mn 99.e'
]")(2-9q .
0 C C) (*mhos/cm) /goo M Temperature pH (SU.) egg._ -5
? .q"~ ')-Aq 2_ - g-C)I J9qoI ,-ý
' -DO (mg/L). ,9*
1000 mg NaClfL Conductivity *
- zo
- NaCIJL 1200 mg Conductivity (irthos/cm) 9*.*U0;3(,,o f
,Temperature -t~q -a 7_q t.
CC) pH (S.U.) ~]'**0 qb,/ "~S Temperature* (°C) z-*... 2S,1 2q.*6. ~ zq, - q -7 DO(mg/L)),7 z
.1400 mg NaCfL. Conductivity-(jnhoskm 31)0Jo 30LO Temperature*
(°C) ' q(, ZO "qq -?S. I. "q.~q. ( -zq , STOCK Conductivity /d . -'-- I(prmhos/cm) C Initial .Inntia Final 'Initial. - Final
Environmental Jesting Solutions, Inc. Species: Ceriodaphniadubia CdNaCLCR#C"R,4: 5S Dav 34 56 Analyst I I )kk -- Concentration Parameter,..." A ft MR, pH(S.U.) P ? Ie ?.e3
?.y-j 8.611 --. 6 DO (rgE) -0 (" .1 CONTROL Conductivity i "
(Hrhos/cm) 361 3 ag 3c2-Alkalinity (gCaCO3/1)
-Hardness (mg CaCO/L)
Temperature p H ( S .U .) 1 ,c-* f . 0 . __2 .6}"2 q/ . DO (mg/L) 8,0 9. 600 mg NaCI/L. Conductivity g ( inhos/cm) /.5/0 'q20 Temperature (0C) T - pH (S.U.) 1.q.e4 5.
*.O - .O"- ,5 DO (mg[L) 14.6. (V 800 mg NaCI/L Conductivity "
( irnhos/cmn) 10502. 0 leo Temperature Is, N. IS2 (0c) DO (mg/) /4 q. q. E3;. 1000 mg NaCVL pH (S.U.) Conductivity .q 4 . q- 69* 7.q5 - ( rnhos/cmn) Temperature - "q *
.()C)
DO (mmg a *LI 1200 mg NaCI/L Conductivity ( mhos/cm.) Temperature. ZLI L Z S(0c) nH (S.UJ. .- 34 q 'Iq -/ c.:i 11-i, -iff - I,-j - 1. I-q DO (mgfL) ý.q Rg 1400 mg NaC1/L Conductivity -Ný,-ý-V (Pnllhos/cm) 9qq/) ýq o :ýý b) 0 %10 Temperature 2Qi. 9 7q, -7 2S5. (0C)
~Lb1
-~ 0 0 -2.1 STOCK T C *
" Conductivity (tirnihos/cm) ,
Initial Final Initial 51 11' Final I I Initial Final Initial Final niil I Fna In...... II *Fnl I nta i na Ini.a Fi na}}