Text

Discharge Monitoring Report for December 2006
	ML070160095
Person / Time
Site:	Sequoyah
Issue date:	01/09/2007
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML070160095 (106)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 January 9, 2007 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR DECEMBER 2006 Enclosed is the December 2006 Discharge Monitoring Report for Sequoyah Nuclear Plant.

Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 N1~TER0FFICE SB-2A)

SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

TN0026450 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE

[

MONITORING PERIOD EFFLUENT Form Approved.

OMB No. 2040-0004 YEAR MO I DAY

ý YEAR MO DAYNO DISCHARGE From 06 12 1

To 06 12 31 NOTE: Read instructions before completing this form.

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information. including the possibility of fine and imprisonment for knowing violations.

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE Ctclclfl~l'O*t-5 I

mi 1

AtTL4tflI71:fI A(PckiT OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. The following information is included in an attachment: 1. thermal compliance information 2. CCW data 3. veliger monitoring data EPA Form 3320-1 (REV 3199)

Previous editions may be used Page 1 of 2 EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 2

December 2006 DMR Attachment December 2006 Thermal Compliance Information December 1-31: The downstream temperature and temperature rate-of-change are based on measurements from Station 8, the "backup temperature monitor" located at the end of the mixing zone (TRM 483.4). The temperature rise is based on the increase in temperature from measurements at Station 14, located upstream of the plant (TRM 490.5), and measurements at Station 8 (TRM 483.4). Consistent with the permit, measurements reported for the downstream temperature and the temperature rise represent daily maximum 24-hour rolling averages; and, measurements reported of the temperature rate-of-change represent 1-hour averages.

Measurements were used rather than the numerical modeling system because of relatively low river flows. TVA has learned as a result of the continuing studies performed under Part III.G. of the permit that under low flow conditions, adjustments may be needed in the formulation of numerical model to improve its accuracy. Until river flows increase or appropriate model adjustments can be made, the modeling system is considered "out of service".

December 2006 CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/01/2006 @ 0735 0.20 mg/L 12/06/2006 @ 1724 LSB EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 12/01/2006 @ 0730

<0.10 mg/L 12/06/2006 @ 1734 LSB EPA 8015 December 2006 Veliger Monitoring Information Sample Date Mean Water Sample Date Mean# of Water LOCATION SUB NOTES:

COLLECTED BY

of Settlers Temp.

Asiatic Temp.

LOCATION ZM/m 3

('C)

Clams/m 3 (,C)

Gravid Asiatic Clam 12/4/2006 0

0 12 12/04/2006 0

12 Inplant RCW Dickie Adcock 12/12/2006 0

0 10 12/12/2006 0

10 Inplant RCW Dickie Adcock 12/18/2006 0

0 10 12/18/2006 0

10 Inplant RCW Dickie Adcock 12/25/2006 0

0 10 12/25/2006 0

10 Inplant RCW Dickie Adcock

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE S B-2A).

SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

L TN0026450 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE MONITORING PERIOD EFFLUENT Form Approved.

OMB No. 2040-0004 I YEAR MO DAY YEAR IMO i DAY From 06 12 01 1 To 1 06 112 131 ATTN: Stephanie A. Howard PARAMETER

- . NO DISCHARGE NOTE: Read instructions before completinq this form.

QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

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<A4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or I

persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is, to the best of my knowledge and belief, true.

423 843-6700 07 01 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

The following injections occured: 1. Biodetergent 73551 (max. calc. conc. was 0.026mg/L--limit 2.0mg/L)

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 2 of 2

PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

-_ __ __._INTEROFFICE SB-2A).

SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01 )

TN06450 FF 101 Q F -FiNAL PERITNUMBER I

DI CHARGE NUMBERI DIFFUSER DISCHARGE Form Approved.

OMB No. 2040-0004 MIMNITflRIIC, PFRIfln I

EFFLUENT hAnK1IT0P1Nr,' PERIOD I YEAR I MO I DAY j From IN o

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER J. Randy Douet Site Vice President TYPED OR PRINTED I Certify under penally of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations.

Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Boron was sampled 10/04/2006 @ 0820.

EPA Form 3320-1 (REV 3/99)

Previous editions maybe used Page 1 of 1 EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

_.INLTEROFFICýE S-A_)

SODDY - DAISY TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESI MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

OMB No. 2040-0004 F

TN0026450 101 T F-FINAL I

PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 MONITORING PERIOD I

EFFLUENT 4YEAR MOI DAY YEAR MO DAY From 06 12 01 1To 10 12 31 NO DISCHARGE NOTE: Read instructions before completino this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted.

wased on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700_07_01_09 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE T information, including the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED coDE COMMENTS AND EXPLANATION OF ANY VIOLATIONS,(Reference all attachments here)

Toxicity was sampled 11/26/06 - 12/1/06. The report is attached. The results were included in the November 2006 DMR.

EPA Form 3320-1 (REV 3/99)

Previous editions may be used Page 1 of 1

January 4, 2007 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2006 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected November 26-December 1, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests.

In addition to the routine compliance test, fathead minnows were also tested in Outfall 1 01 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah.

Call me at (256) 386-2755 if you have any questions or comments following your review of the report.

Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment):

R. M. Sherrard, PSC IX-C Files, RSO&E-EDMS-Muscle Shoals SQN November 2006M-second test

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: January 4, 2007

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1579

7. Receiving Stream: Tennessee River (TRM 483.6)

8.

1Q10: 3491

9. Outfall Tested: 101

10. Dates Sampled: November 26-December 1, 2006

11.

Average Flow on Days Sampled (MGD): 1528, 1493, 910, 908, 911, 915

12.

Pertinent Site Conditions:

Towerbrom 960 (chlorination) was injected from November 26 - December 1, 2006. The dates and times for the Towerbrom 960 injection are in the following table.

Injection Location Date/Start Time (ET)

Date/Ending Time (ET)

Essential Raw Cooling Water (ERCW) 11/26/2006 0000 12/01/2006 2400 Train A & B 11/26/2006 0000 11/27/2006 1600 Raw Cooling Water (RCW) 12/01/2006 1142 12/01/2006 1600

13. Test Dates: November 28-December 5, 2006

14. Test Type: Short-term Chronic Definitive

15. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

16. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0

17. Permit Limit Endpoint (%): Outfall 101: IC25 = 45.2%

18. Test Results: Outfall 101: Pimephales promelas." IC2 5.> 100%

Ceriodaphnia dubia: IC25-5.> 100%

UV treated Outfall 10 1: Pimephales promelas: IC2. > 100%

19. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6700

20. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

21. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

22. TVA

Contact:

Cynthia L. Russell Phone #:. (256) 386-2755

23. Notes: Outfall 101 samples collected November 26-December 1, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant differences from the control during this study period. Growth in minnows exposed to intake samples was significantly lower than minnow growth in the control.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/

(MM/DD/YY)/

Arrival Initial (MM/DD/YY)/

Sample Time (ET)

Time (ET)

Temp.

TRC*

Time (ET)

ID Collected Received

(-C)

(rg/L)

First Used By 101 11/26/060753 to 11/27/06 1400 3.7 /3.4 t

<0.10 11/28/06 1559 11/27/06 0653 11/29/06 1507 11/26/06 0809 to 11/28/06 1559 Intake 11/27/06 0709 11/27/06 1400 1.4

<0.10 11/29/06 1507 11/27/06 0709 11/29/06 1507 101 11/28/06 0826 to 11/29/06 1400 4.3 /4.6t

<0.10 11/30/06 1510 11/29/06 0726 12/01/06 1513 11/28/06 0843 to 11/30/06 1510 Intake 11/29/060743 11/29/06 1400 1.1

<0.10 12/0/06 1513 11/29/06 0743 12/01/06 1513 12/02/06 1507 101 11/30/06 0758 to 12/01/06 1350 4.5/5.2'

<0.10 12/03/06 1511 12/01/06 0658 12/04/06 1512 12/02/06 1507 11/30/06 0813 to120/657 Intake 11/0/06 0813 12/01/06 1350 2.5

<0.10 12/03/06 1511 12/01/06 0713 12/04/06 1512

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.02C) in a warm water bath.

Aliauots of Outfall 101 and Intake samnles were UV-treated through a 40-watt SmartiUV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

3

Test Organisms:

Pimephales promelas Aquatic BioSystems, Inc.

Ceriodaphnia dubia In-house Cultures

1. Source:

2. Age:

24-26 hours old

<24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5. Organisms per Replicate: 10

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101

7.

Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101

8.

Test Temperature: Outfall 101:

10 I

11/28/06-1559 ET 11/28/06 - 1547 ET 12/05/06 - 1600 ET 12/05/06 - 1545 ET Mean = 24.62 C (24.1 - 25.12°C) 11/28/06 - 1042 ET 12/05/06 - 0954 ET Mean = 24.8 2C (24.5 - 25.2-C)

Test Temperature: UV-Treated Outfall 101:

Mean = 24.72C (24.1 -25.1V-QC

9. Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 28-December 5, 2006 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - da s)

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 100 100 100 100 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 98 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

Test Efluetis (replicate number)

(%Effluent) 1 2

i 3

4 Mean Control 0.707 0.726 0.706 0.682 0.705 11.3%

0.644 0.641 0.682 0.601 0.642 22.6%

0.668 0.650 0.667 0.692 0.669 45.2%

0.649 0.745 0.637 0.677 0.677 72.6%

0.600 0.618 0.628 0.588 0.609 100.0%

0.536 0.595 0.571 0.666 0.592 Intake 0.614 0.593 0.615 0.637 0.615 IC 25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/ IC 25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 28-December 5, 2006 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days Solutions 1

2 3

4 5

6 7

(% Effluent)

Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100 45;2%

100 100 100 100 100 100 100 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(%Effluent Data (replicate number)

(%Efet) 1 2

3 4

5 6

7 8

9 10 Mean Control 25 27 31 28 28 27 28 29 29 30 28.2 11.3%

26 28 32 29 28 32 33 28 29 29 29.4 22.6%

29 30 29 32 29 31 30 31 31 31 30.3 45.2%

31 32 31 32 29 28 30 30 32 29 30.4 72.6%

28 35 31 30 28 34 31 36 30 28 31.1 100.0%

31 31 30 33 30 36 30 37 29 32 31.9 IC2 5 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 45.2%

Permit Limit: 2.2 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50: TUc = 100/IC 25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 28-December 5, 2006 using water from Intake Percent Surviving Test (time interval used-- days)

Solutions 6

7

(% Effluent) 1 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Ts Sfluetis Data (replicate number)

(%Effluent) 1 2

3 4

5 6

7 8

9 10 Mean Control 28 25 25 28 30 26 30 28 28 28 27.6 Intake 32 28 31 33 29 28 32 30 31 30 30.4 IC 25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC 50: TUc = 100/IC25 7

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted November 28-December 5, 2006 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2

3 4

5 J 6 7

Control 100 100 100 100 100 100 100 11.3%

100 100 100 100 100 100 100 22.6%

100 100 100 100 100 100 100 45.2%

100 100 100 100 100 100 100 72.6%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)

Test Soluetios (replicate number)

(% Effluent) i 2

3 4

Mean Control

.0.565 0.645 0.669 0.763 0.661 11.3%

0.645 0.738 0.661 0.656 0.675 22.6%

0.597 0.602 0.630 0.709 0.635 45.2%

0.634 0.604 0.513 0.602 0.588 72.6%

0.542 0.583 0.583 0.630 0.585 100.0%

0.637 0.705 0.748 0.623

.0.678ý Intake 0.619 0.608 0.667 0.682 0.644 IC 2 5 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0: TUc 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration. Toxicant Results (IC 25 November 28 -

Pimephales promelas December 5,2006 1609 7-days KC1 0.63 g/L Ceriodaphnia dubia November 7 - 14, 2006 1127 7-days NaC1 1.06 g/L 8

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, November 28 - December 5, 2006 Test Sample ID Temperature ('C)

Dissolved Oxygen (mg/L) pH (SU.)

Conductance Alkalinity Hardness Total Residual Initial Final

. Initial Final Initial Final (pmhos/cm)

(mg/L CaCO03) (mg/L CaCO3)

Chlorine (sag/L)

Control 24.7 24.5 7.7 7.3 7.76 7.17 310 58 89 24.5 24.8 24.2 24.8 7.6 7.8 7.1 7.7 7.59 -

7.90 7.03 7.35 300 318 58 59 86 92 11.3%

24.8 24.4 7.8 7.3 7.50 7.13 293 24.7 24.8 24.2 24:8 7.6 8.1 6.9 7.7 7.28 -

8.00 6.92 7.31 284 304 22.6%

24.8 24.4 7.8 7.2 7.49 7.12 280 F_

24.7 -

24.9 24.2 -

24.6 7.6 8.1 6.7 7.5 7.27 -

8.00 6.93

- 7.26 273 289

4 24.8 24.5 7.9 7.1 7.47 7.13 255 45.2%

24.7 -

24.9 24.2

- 24.9 7.6

-.8.2 6.4 7.5 7.25 -

8.01 6.89 -

7.27 248 261 24.9 24.4 7.9 7.2 7.45 7.14 222 72.6%

24.7 -

25.0 24.1 -

24.9 7.7 8.2 6.3 7.6 7.23 -

7.99 6.88 -

7.27 215 234 100.0%

24.9 24.5 8.0 7.2 7.43 7.16 189 66 81

< 0.10 24.7 -

25.1 24.1

- 25.0 7.7 8.2 6.2 7.6 7.20 -

7.97 6.89 7.29 185 192 66 66 78 86

<0.10 -

<0.10 Intake 24.8 24.5 8.0 7.3 7.40 7.15 190 65 81

< 0.10 24.7 -

25.0 24.3 24.9 7.7 8.2 6.6 77 7.17 - 795 6.91 7.30 184 192 64 67 80 84

<0.10 -

<0.10 Control 24.7 24.9 7.7 7.7 7.76 7.36 310 58 89 24.6 -

24.8 24.7 25.2 7.6 7.8 7.5 7.9 7.59 -

7.90 7.21 7.50 300 318 58 59 86 92 24.7 24.8 7.8 7.8 7.50 7.35 293 24.6 24.8 24.6 -

25.0 7.6 8.1 7.5 7.9 7.28 -

8.00 7.20 7.49 284 304 26 24.8 24.8 7.8 7.7 7.49 7.35 280 24.7 24.9 24.6

-25.0 7.6 8.1 7.5 7.9 7.27 8.00 7.20

- 7.50 273 289 42%

24.8 24.8 7.9 7.7 7.47 7.35 255---

4.

24.7 -

24.9 24.5 25.1 7.6 8.2 7.4 7.9 7.25 -

8.01 7.21 7.51 248 261 24.9 24.8 7.9 7.7 7.45 7.35 222

-2 72.6%

24.7 -

25.0 24.6 24.9 7.7 8.2 7.3 7.9 7.23 -

7.99 7.20 -

7.49 215 234 100.0%

24.9 24.8 8.0 7.6 7.43 7.35 189 66 81

<0.10 24.7 -

25.2 24.6 25.0 7.7 8.2 7.2 7.9 7.20 -

7.97 7.22 -

7.49 185 192 66 66 78 86

<0.10 -

<0.10 Intake 24.9 24.8 8.0 7.8 7.40 7.35 190 65 81

<0.10 Intake 24.6 -

25.1 24.7 25.0 7.7 8.2 7.6 8.1 7.17 -

7.95 7.21 7.50 184 192 64 67 80 84

<0.10 -

<0.10 Overall temperature (C)

Pimephales promelas

-Ceriodaphnia dubia Average 24.6 24.8 Minimum 24.1 24.5 Maximum 25.1 25.2 9

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, November 28 - December 5, 2006.

Test Sample ID Temperature (C)

Dissolved Oxygen (mg/L) pH (S.U.)

Conductance Initial Final Initial Final Initial Final (gimhos/cm)

Control 24.8 24.5 7.9 7.5 7.45 7.18 300 24.6 -

24.9 24.2 24.9 7.7 8.1 7.0 7.8 7.25 -

7.91 7.11. -

7.31 294 311 11.3%

24.8 24.4 7.9 7.4 7.45 7.17 294 24.7 25.0 24.1 24.8 7.7 8.1 6.8 7.9 7.25 -

7.91 7.08 -

7.28 288 -

305 24.9 24.5 7.9 7.4 7.45 7.17 280 ZZ22.6%

24.8 24.9 24.2 24.8 7.7 8.1 6.9 7.7 7.25 - 7.90 7.07 - 7.28 275 290 24.9 24.5 7.9 7.3 7.44 7.15 257

45.2%

24.8 25.0 24.2 24.9 7.8 8.2 6.8 7.6 7.24

-7.90 7.05 7.25 253 263 72.6%

24.9 24.5 7.9 7.3 7.4.2 7.16 223 24.8 25.1 24.2 24.9 7.8 8.1 6.7 7.8 7.20 7.90 7.06 - 7.29 218 230 100.0%

25.0 24.5 7.9 7.2 7.41 7.17 192 24.8 25.1 24.2 24.9 7.6 8.1 6.6 7.6 7.19 7.92 7.06 7.29 190 194 Intake 24,9 24.4 7.9 7.3 7.37 7.15 188 24.8 25.0 24.3

-24.7 7.6 8.1 6.7 7.6 7.16 7.91 6.96

-7.25 185 195 Overall temperature (°C)

Pim ephales prom elas Average 24.7 Minimum 24.1 Maximum 25.1 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected November 26-December 1, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of daphnids to intake samples resulted in no significant differences from the control during this study period. Growth in minnows exposed to intake samples was significantly lower than minnow growth in the control.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.

11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promnelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None 12

PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360.1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8.

Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by ORION 97-70.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 valuesin g/L KC1 or NaCI.

2.

Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas.

Sodium Chloride (NaCI crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821 -R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPA-600-4-79-020 (March 1983).

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring November 28 - December 5, 2006 Appendix B Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1,2006 Date

$editim Towerbrom PCL-222 PCL-401 CL-.363 Cuprostat-W30*

Hyot*

oite mgiL mifL mg/L PF LF

)3 /1 2 /19 9 8 3/13/1998 i

)3/14~4 99 lZ=

=

=:

)3 /1 5 /1 9 9 8

)3/12/1998 :...:-::,

)3 /13 /19 9 8 *.......

)9/08/1998 0*::ii:::::::::::*i::..0.1$

0014ii~iiiii{ii{*

((ii~iiil 0.005

&0iiili lii i l i ii i~ii 21 ~~i

)9/09/1998 0003ii*

iiii 003~iiii iii*iiii{*~~l*

iiiiiiiii{*

0.011 ii:*::**:i:*::*:i::

)9/10/1998 iiiii{*i t iiiiiiii ii{iiiiiiiii

{* {iiiiiiiiiii 0.1 0 6 0219 iiiiiiiiiia.iiiii!iii i:ii:i{::iiiii::i::i

)9/13/1998

<Et0&iiiii~iii{ii**

i 0044i{i}

i!i~i {

!!}((ii 0.015 ii(({i((ii((i~ii~ii~i

)9/14/1998..

00 0015 2/22/1999...

12/24/1.999 00iiiiii~~ii~iiii*

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~ ~ ~ ~

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18/20/1999 :::::::::::::::::::::::::::::::::::::::::::

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0.024 0

)3/18/1998 0016..... 0........

7..

0.023 0...-

I

Table 13-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 10 1) Discharge Concentrations of Chemicals Used to Control Growth of Mi crobio logically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Dae iiii~iiii:ii~

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T C co.l..

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Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall I 0 1) Discharge Concentrations of Chemicals Used to Control Growth of Microbi ologic ally Induced Bacteria and Mollusks, During Toxicity Test Sampling, I March 12, 1998 - December 1, 2006 ý...................

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Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date i~*i!* ~ ~ !!*Towerbrom iiiiii PCL-401 ii * .:iii Cuprostat-i:...*.i

iiiiii~{***i*ii!{i{~ ii~ is ***{i~~~~~iiiii~~~~~ii~ii{ii A o l 06/1

/200 .}...... 0...iiiiii~~ii!i!i*i~~*i~iiii~iiii~~i~i* iiiii iiiii~i{i~ii{iiiiiii{i{i~((:... ......i~~~~iii~~~~i!i~~ J.::: 0 8 0 /2 0 i::~iii!:~~iiii~~:*.:... 0 5..:

~:i~iiii~:**........... *............

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iii{

(({ 10/0 /200 <0 0090

i * * *{ {*{* {i~ii}*((*{ii{

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Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date iiiiiiii iiiiii Towerbrom... PCL-401 ii ii Cuprostat-PF !I*i i Nalco i iiii~iii:ii~iiiiiiiiiii TRC i-i*iiCopolymer i il Azole iii~ ii!i mg/L iiiii~i~ 11/107/2004 * {

i :.iii

<0,0187

iiiii'i~
ii~iiii 0.014

si**i**iii!i

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l 0.0 1 6

!!i!:* ii*:::~ iiii i * : } i ii: 11/10/2004 <0.025149 ii~i ii~* iii~iii~ 0.0176 ~ iii~*! i~iii~ i 11/11/2004 <.149

ii~i}*~ iii~~

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*((i~ii!i 0.017 i~::i~ii!!!!:::

ii~ 06/06/2005~~...... 0 6/07/2 00 5 0.0 10 3 ~............ iiiiiiiiiiiiiiiiiii 02/06/2005 <~~~~~i*:}{*0.00429 i!;~~i 02/107/2005 0.007i:~i:ii*i:~iiiiiii iiiiii**:i~ ii:iiii~i ........................ iii....... 0.0 0....... 0 7/1 /2 00

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10 9.............. 02/09/2005 0.016399 iiiiiiig i iii{ 0.0109{ ~i!~i*. 0 7/2 /2 00 0 0 24 2 {iiiiiii {g * {iiii 0.0 9 i'i'? ???' 72"2"2""i "':+:'................'

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- a 5

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 ate iiiiii i{Towerbrom i:i::i*

~~:i:

PCL-401 i:!*i iiiCuprostat-PF i N lo li ti }* i mgiL i iiiii*

ii~ii mg/L iii ii" mg/L iiii ii 73551 iii iiiiiiiiiii~ii*i o

~

0 0 *~~...............

i i i i~ iii i i ii 1/200 iii *~iliijii ii~i~iii...........018 X. X............. 6

Sequoyah Nuclear Plant Biomonitoring November 28 - December 5, 2006 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I U w Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 FedEx UPS Bus Delivered By (Circle One): Other (specify): Express Courier General Comments: Client Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Fax: 828-350-9368 Collected By: Chevy Williams, Roy Quinn I Chevy Williams 11/27/06 Express sQýrier 11/27/06 Express Courier 11/27/06 ETS 11/27/06 112/6 T /* £ a,n1/70 14 00 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 61C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification/ Grab/Comp. Collection Date/Time Container Flow RiE.O? .d14 "O': Sample Description Number & MGD Rain Event?Use Volume (Mark as Appropriate) Laboratory Use Collected Ye -riva Time. By.e.. rT~Y /~,r*g.ate u.: Yes If Yes, No Trace ETS Log Aival Temp By Time Appear ~ _______________ ~______ Inches ___Numiber _____) ance SQN-101-TOX Comp 11/28/06-11/29/06 0826-2 (2.5gal) NA o 43 1 9 'A-SQN-INT-TOX Comp 11/28/06-11/29/06 0843-1 (2.5 gal) NA .400 0743-. Sample Custody - Fill In From Top Down

42 ta4-i Relinquished By (Signature):

Date/Time Received By (Signature): Date/Time C evy WilliamsQ &\\ 11/29/06 Express Courier 11/29/06 Express Courier 11/29/06 ETS 11/29/06 .t-v- -g Nubi Instructions:. Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page -_ of _1 U p.. I Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 Delivered By (Circle One): FedEx UPS Bus Client Other (specify): Express Courier General Comments: Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 9 I I. /35-0 + A -A Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time fbr eact sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Sample. shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testing Solutions, Inc. Pace I of 6 Environmental Testing Solutions, Inc. Page I of6 Chronic Wvhole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #:

2qtO, County: Hamilton.

Treatment: Non-treated Outfall: 101 Dilution oreparation information: Dilution prep (%) 11.3 Effluent volume (mL) 282.5 Diluent volume (mL) 2217Ti Total volume (mL) Test organism information: Test information: Organism age: 2k -ro--X r A d'4* oa-," Randomizing template: qeuoO Date and times organisms i\\- Z.71-o', k4e -rM too Incubator number: were born between: Organism source: p:

-cs (tv 7-t -

Artemia lot number: 6-vz*q Transfer bowl information: pH = Temperature = C Total drying time: Z- -S

l. to

-Z. Date / Time in: 1--(,- o .i 'w. Average transfer volume: S. Date /Time out: t2-o,-006 16 Z,-L Oven temperature: (c,O Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0____ 'SS0 9 Ž v.-( 0 I UL.-, A 0 1-21., I -4 0.- 4____._" 40 I'E 1 l-I-e%,O¢ a'* l 0.,Ot~ ~ L 5 /b5aq)3,O Cqr-* '$-Qo 1$!, 12 -c2..-0 (A OL,,tzo.0\\ I '* OLCPL 46 l

Oqo,

,q iCJ i' s2-o_-oA 06,8zoLO0 4 0L 5_____ 011-12~ -loO g-- A 1t o i ý Control information: Acceptance criteria Summary of test endpoints: % Mortality: <20% 7-day LC50 1 Average weight per initial larvae: O.105 .>J , NOEC < it - 7., Average weight per surviving larvae: O.10S -0.25 mg/larvae LOEC 4 IIY7. {'*hV -< 11. j,"7 ChV <L~

-Ves (ZeSOLTSc Ak.e UOP.eLJA-&.Q.

Environmental Testing Solutions, Inc. .Page 2 of 6 Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: Ji'Re6-o* Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G [H I J K L l0 1 o /0 tO) I 0 © 10 AD 10 10 10 / 0 lO /0 10 to Io /0 /o Io 1/ t JO 1 /0 I0 I0 to /0 /0 10 10 10 D 0 /0 tO 2 /0 /0 /0 /0 t0 10 /0 /0 /0 /0 /( /6 5 /0 /0 /0 /00 / /0 /0 /0 /0 /0 /(D /' 6 /0 io /0 /0 /0 /0 /0 /0 /0 /0 /"0 /0 7 10 0 /0 10 /1 10 tO /0 /0 /0 /0 /a A = Pan weight (mg) Color identification:*10aCk-1Va.N It*.Rq/ 1q.3) 15.3cl 13.59 13.50 Iq.ra 1q,39 RiR~o /MB. 1q.9q IIIt' 4.1 9 t/I Analyst: t.JV > B =Pan + Larvae weight (mg) 91.3b 61151 caa.39,ao. tjq.' q al-l3 dl~l-K a0,13 ;a1.5a1 ai.49 ao.Bi at-i Analyst:

Dt a; Larvae weight (mg) = A - B 17,o1

-1..2t, -. ot-6,R.t (.A l

t.

k k,., t..O k t s-*o Weight per initial number of larvae (mg) = C / Initial number of larvae 'N 0" ,,P 0" IV C4. ACV 0, 0" C, O0 4)9 V.. I0V Average Percent weight per reduction initial from control 0D10 number of (%) larvae (mrg) .(A-z 9,0 07 0, to 61 -S.\\* 7. 6 I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m.= missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments-PiA1-AnC4ke*_ WA",a'n tain10V %te?,YrFkP_j1 C -n4*. 7 ¢AR_¢ ^ct e Gt-L ae u

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: I;'*c& Survival and Growth Data Day 45.2% 72.6% 100% M N 0 P Q R S T U V W X 0 ju ic /L) IL j0 1 j)0 10 C) () o0 1 1 10 10C) to 10 10 10 to I D Io b 10 ,o 2 10 10 (0 /0 /0 t o0 0 /0 /0 3 10 /0 1 0 t 0 /0 I0 l0 to 10 (0 /0 10 4 (0 I0 /0 to /0 lo 10 i0 10 1 0 10 5 50 IL /0 1/ 10 /0 /0 /0 (C)

0) fO

/0 6 /0 10 / C )0 /0 /0 /0 / /0 /0 /0 1' I /0 /0 10 10 to C) 10 1 T3 10 A : Pan weight (mg) Color identification: biO a ¢- 1 13.58 RlI~S Iq.Oq, 1q-59~ J4.65 13.(c14 1q.49 13.bq 15.()q 13.1ý1 t'a(O 1.101 Analyst: U'16 B = Pan + Larvae weight Analyst: g)(.o9 ac.0 a -9 O,4i a.36 a0.b 19.Ba ao.v779.a ao.4 19.1Q ao.qi a1.3(c Larvae weight (mg) = A - B (,,Aqt 6A, 11 t.77 6.O 6,\\$, 16.I 5.3t1 5.15 S.-It 1,,- Weight per initial number \\. of larvae (mg) ON \\ o C / Initial number of larvae -1,

1P 0*"

0" 0" 0b t" 0 ob ob b Average Percent weight per reduction initial from control

4. o 0.to 0.,

number of (%) larvae (mg) Comment codes: c = clear, d dead, fg = fungus, k killed, m = missing, sk sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions. Inc. Page 4 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: I, 28.0(0 Survival and Growth Data Day 100% Intake Y Z AA BB 0 (0 t( /0 /0 2 3 (C) 0 C( /0) /0 t0 /0 /0 7 6 /0 /0 A Pan weight (mg) Color identification k'lOdc 1q.30 Iq.45 .15 R IS1.O Analyst: I 1t B= Pan + Larvae weight (mrg) clO.4q 0-,0.38,D.30 @2j.. Analyst: LV3 0,1)o.8 Larvae weight (mg) = A - B Weight per initial number of larvae (mg) C / Initial number of larvae '\\9 "o Average Percent weight per reduction initial from control 0.6ks 12. number of (%) larvae (mag) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimeph ales promnelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2906 Reveiwed by: /- Not for Compliance Assessment, Internal Laboratory QC Ccmatn-leon( (%) Rep[ c*.-toIi canabarof FI-rsiumb-rofltcvco A=Pa.-ctht(n,) B-Pa. La*~a, Laa wIeght (ing) WtihSrciuen M anatehtlSarciaia CSumrh., van.ofzu/ Witda/lIdtialnumb-erf Ma.nacrcivi Moanwtzl/ fnJtio. Co.tfkiatnofva-ri*a*.

p.

tntcn

rrae, larva-wlghl (-ag3 A-B

-ube toan' (n3

cau, roftarva. (ma} PA..al0ae..suro.d br ta.

(aql (%W number artara'.. (nil)aut uea ntalt (%) ot: o( )(fat) '..b,rOOt a.,) (%) A 10 10 14.29 21.36 7.07 0.707 0.707 Control B 10 10 14.31 21.57 7.26 0.726 0.705 2.6 0.726 100.0 0.705 2.6 Not applicable C 10 10 15.32 22.38 7.06 0.706 0,706 D 10 10 13.59 20.41 6.82 0.682 0682 E 10 10 13.50 19.94 6.44 0.644 0.644 11.3% O F 10 10 14.72 21.13 6.41 0.641 0.642 5.2 0.641 100.0 0.642 5.2 9.0 G 10 10 14.34 21.16 6.82 0682 0.682 1I 10 10 14.12 20.13 601 0601 0.601 1 10 10 14.84 21.52 6.68 0,668 0.668 22.6% J 10 10 14.97 21.47 6.50 0.650 0.669 2.6 0.650 100.0 0.669 2.6 5.1 K 10 10 14.14 20.81 6.67 60.667 0.667 L 10 10 14.19 21.11 6.92 0.692 0.692 M 10 10 13.58 20.07 6.49 0.649 0.649 45.20/ N 10 10 14.45 21.90 7.45 0.745 0.677 7.1 0.745 100.0 0.677 7.1 4.0 o 10 10 14.04 20.41 6.37 0.637 0.637 P 10 10 14.59 21.36 6.77 0.677 0.677 Q 10 10 14.85 20.05 6.00 0.600 0.600 72.6% R 10 10 13.64 19.82 6.18 0.618 0.609 2.9 0.618 100.0 0.609 2.9 13.7 S 10 10 14.49 20.77 6.28 0,628 0.628 T 10 10 13.84 19.72 5.88 0.588 0.588 U 10 9 15.04 20.40 5.36 0.596 0.536 100% V 10 10 13.81 19.76 5.95 0.595 0.607 6.8 0.595 97.5 0.592 9.3 16.1 W 10 10 15.26 20.97 5.71 0.571 0.571 X 10 10 14.70 21.36 6.66 0.666 0.666 Y 10 10 14.30 20.44 614 0.614 0.614 100% Intake Z 10 10 14.45 20.30 5.93 0.593 0.615 2.9 0.593 100.0 0.615 2.9 12.8 AA 10 10 14.15 20.30 6.15 0.615 0.615 BB 10 10 16.01 22.38 6.37 0.637 0.637 Outfall 101: Dunnetts MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 0.0598 8.5 0.0247 3.5 MSD = Minirtssm Significant Difference PMSD Percent Misirrmm Significant Difference PMSD is a measure of test precision. The PMSD is the minirmumpercent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a sigaificant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.5% fromthe control (determined through reference toxicant testing). Lower PMSD bound deteirmited by USEPA (10th percentile) 9.4% Upper PMSD bound deterrnined by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPAusing 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. USEPA. 2000. Understandingand AccountingforMethod Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, O.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/28/2006 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Outfall 101 End Date: 12/5/2006 Lab ID: LMr-Envir. Testing Sol. Sample Type: DMR-Dzcharge Monitoring Report Sample Date: Protocol FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales proinelas Comments: Non-loeated Conc-% 1 2 3 4 D-Control 0.7070 0.7260 0.7060 0,6820 11.3 0.6440 0.6410 06820 0.6010 22.6 0.6680 0.6500 0.6670 0.6920 45.2 0.6490 0.7450 0.6370 06770 72.6 0.6000 0.6180 0,6280 0.5880 100 0.5360 05950 0.5710 06660 Transform: Untransformed 1-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stlt Critical MSD Mean N-Mean D-Control 0.7053 1.0000 07053 0.6820 0.7260 2.556 4 0,7053 1.0000

-13 06420 0.9103 0,6420 06010 0,6820 5.155 4

2.548 2.410 0.0598 0,6628 0.9397 22.6 0.6693 0.9490 0.6693 0.6500 0.6920 2.580 4 1.450 2410 0.0598 0.6628 0.9397 45.2 0,6770 0.9599 06770 0.6370 07450 7.139 4 1 138 2.410 0.0598 0.6628 0.9397

72.6 06085 0.8628 0.6085 05880 0.6280 2.944 4

3.897 2410 0.0598 0.6085 0.8628

100 05920 0.8394 0.5920 0.5360 06660 9.284 4

4.562 2410 00598 0.5920 0.0394 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.942544222 0.884 0.702791759 0.883183654 Bartlett's Test indicates equal variances (p = 0.21) 7.118869305 15.08627224 ,Hypothesis Test (1-tail, 0.oS) NOEC LOEC ChV TU MSDu MSDp: MSB MSE F-Prob df Dunniett's Test <11.3 11.3 0.059831485 0.08483727 0.0074371 0.001232694 0.0018978 5,18 Treatnents vs D-Control Linear ]nlerpolation (200 Resarnple5) Point SD 95% CL(Exp) Skew 1C05" 9 376 16.565 4.511 76.887 1.2631 ICIO 59,355 ICIS 87.608 1.0 IC20 >100 0.9 IC25 >100 1C40 >100 0.8 1C50 >100 0.7 indicates IC estimate less than the lowest concentration a*0.6. o 0.5 0*4 0.3 0.2 0.1 0.0 0 20 40 60 O0 100 120 Dose % Dose-Response Plot 0.8 0.7 0.6 0.5-O 0.4 003 02 0.1 1-tall, 0.05 level of sgrnificance -6 in .sqn_l 06dato

TVA / Sequoyah Nuclear Plant, Intake Non-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11128/2006 Test ID: PpFRCR Sample ID: TVA/Sequoyah Nucleacr Plnt, Intake End Date: 12/5/2006 Lab ID: ETSi-Envir. Testing Sol Sample Type: DMR-Discharge Monitoring Report Simple Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales pramelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.7070 0,7260 o.7060 0.6820 100 0.6140 0,5930 0.6150 0.6370 Transform: Untransformed I-Tailed Isotenic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Conttol 0.7053 1.0000 0.7853 0.6820 0.7260 2.556 4 0.7053 1.0000

100 0.6148 0.8717 0.6148 0.5930 0.6370 2.923 4

7.T11 1.943 0.0247 0.6148 0.8717 Auailiary Tests Statistic Critical Skew Kurl Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.883914053 0.749 -0.1195042 -0.69030905 F-Test indicates equal variantces (p = 1.00) L.006193519 47.46722794 Hypothesis Test (1-tail, 0.089 MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.024729467 0.035064824 0.0163805 0.000323917 3.9E-04 1, 6 Treatments vs D-Conmol Linear interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05* 38.964 5.006 24,112 63.702 0.8889 1C109 77.928 IC15 >100 1.0 1C20 >100 0.9 IC25 >100 IC40 >100 0.0 IC50 >100 0.7 indicates IC estimate less than the lowest concentration a0.6 o0.5 0.4 0.3 0.2 0.1. 0.0 0 20 40 60 80 100 120 Dose % Dose-Response Plot 0.0. 0.7 1-tail, 0.05 level of significance 0.6 o as 05 0.4 0.3 0.2 0.1 0 o cl ot sqn_ll-28-O6data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 2906 Reviewed by: Concentration Parameter Da 0 Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Initial Fin atl Final Initial Final Initial Final Initial Final Initial I Final PH(SU) 7.69 7.35 7.75 7.03 7.59 7.17 7.82 7 7.89 77.90 7.04 7.651 7.221 DO (m rg/L) 7.7 7.7 7.8 7.1 7.7 7.4 7.6 7.7 7.8 7.1 7.8 7.3 Cnrl Conductivity (Iimhos/cm) 318 318 31.5 307 34300M30 Control Alkalinity (mg/L CaCO3 ) 58 59 58 Hardness (mgfL CaCO3) 92 86 90 Temperature (C) 24.6 24.5 24.5 24.6 24.7 24.8 24.81 24.5 24.7 24.6 24.8 24.2 24.6 24.4 pH (SU) 8.00 7.31 7.43 6.92 7.28 7.11 7.33 7.13 7.44 7.13 7.66 7.02 7.38 7.26 3 DO (m 7.7 7.7 7.8 6.9 5 7.6 7.3 7.8 2 7.8 8.1 7.0 8.1 7.4 Conductivity (Itmhos/cm) 301 I 304 295 286 289 284 290 Temperature ( °C) 24.8 24.2 24.7 24.8 24.8 24.8 24.5 24.7 24.4 24.8 24.5 24.7 24.2 pH (SU) 8.00 7.26 7.42 6.93 7.27 7.12 7.32 7.141 7.43 7.14 7.64 7.02 7.38 7.261 26% DO (mg/) 7.7 7.5 7.8 6.7 7.6 7.2 7.8 7.21 7.8 7.1 8.1 708.1 7.4 Conductivity (ltmhos/cm) 287 289 282 275 275 273 277 Temperature ( °C) 24.8 24.2 24.7 24.5 24.8 24.6 24.8 24.4 24.7 24.4 24.9 4.3 24.7 24.2 pH (SU) 8.01 7.27 7.40 6.89 7.25 7.14 7.29 715 7.43 7.14 7.58 .07 7.36 7.26 DO (mg/L) 7.7 7.5 7.8 6.4 7.6 7.4 7.9 7.2 7.8 7.0 8.2 6.9 8.1 7.5 45.2% Conductivity (tmhos/cm) 260 261 256 260 248 251 252 Temperature ( °C) 24.9 24.2 24.7 24.7 24.9 24.9 24.9 24.6 24.7 24.3 24.9 24.6 24.7 24.3 pil (SU J) 7.99 7.27 7.39 6.88 7.23 7.13 7.27 7.16 7.38 7.18 7.56 7.07 7.35 7.26 76% DO (mg/L) 7.7 76 7.7 6.3 7.8 7.4 8.0 7.3 7.8 7.0 8.2 7.0 8.17. Conductivity (Itmhos/cm) 234 225 22? 218 215 218 222 Temperature ( °C) 25.0 24.3 24.8 24.6 24.9 24.9 24.9 24.4 24.8 24.6 24.9 24.2 24.72 pH (SU) 7.97 7.29 7.40 6.89 7.20 7.15 7.22 7.16 7.38 7.18, 7.49 7.17 7.32 7.27 DO (m ) 7.7 7.6 7.71 62 7.9 7.4 8.21 7.2 8.0 7.0 8.21 7.31 8.1 Conductivity (jLmhos/crn) 189 19 192 18 1881819 100% Alkalinity (mg/L CaCO 3) 66 66 Hardness (mg/L CaC03) 78 86 80 Total Residual Chlorine (mg/L) <0.1 <0.10 <0.10 Temperature CC) 25.1 24.6 24.9 24.6 25.0 25.0 24.91 24.4 24.8 24.6 24.9 24.1 24.7 24.1 pH (SU) 7.95 7.30 7.39 6.91 7.17 7.16 7.20 7.19 7.32 7.11 7.44 7.08 7.32 7.27 DO (mg/L) .7.7 J 7.71 7.91 6.6 7.91 7.51 8.21 7.51 8.01 7.11 8.21 7.41 8.21 7.5 Conductivity (pmhos/cm) 99 i 189 100% Intake Alkalinity (mg/L CaCO3) 65 67 Hardness (mg/L CaCO3) 80 84 Total Residual Chlorine (mg/L) <0. <10 <0.10 _Temperature .(C) 25.0 24.4 24.7 24.6 25.0 24.9 24.8. 24.8 24.4 24.9 24.3 24.7 24.3

Environmental Testing Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistr: Date: JI, .ce

Day Analvst f6--

l 'kl-I ý'. 1

t Concentration Parameter CONTROL pH (S.U) ta.*

q -35 4 _"1 II I DO (mgL) ] ";4.e) [.*"-. Conductivity Alkalinity 6 o Hardness 9--9 (mg CaCO3IL) Temperature(0 C) ( AJO i'Q.S 1 4S -A.to _V.A v4,.8 pH(SU.) 6-OO h4.31 q.q43 (PICA,4.2,9 'I -.1 11.3% DO (mg/L) Conductivity (ýimhos/cm) 30I Temperature('C) 1I-1-' I______ pH (S.u.) 6.* .Lx, U.* .14 3,q 1rm/ 9 22.6% DO (mg/L) 2 Conductivity (Pmhos/cm) Temperature(°C) "4., t I

.1

!I P, 1)-S I -t*4.o pH(S.U.)

0.

I I ý.Ll 45.2% DO (mg/L) Conductivity (Pmhos/cm) Temperature (°C) z'q.9 tL - 7. 7, 1'_ __1_ _1 pH (S.U.) 1.943q II 72.6% DO (rng/L) ,0 Conductivity (pmhos/cm) M _3+'0 C°on ductivi ty I* Lq'* Iq (mg CaCO3/L) (mag CaCO3/L) TR chlorine <0,00 J (mg/L) Temperature('C) 15.1 z.q 6 14 "S.O pH(S.U.) r96 14.3o l, t 6 7-1,(o 100% Intake DO (rag/L) Conductivity (iimhos/cm) Alkalinity (5 (mg CaCO3/L) Hardness 0o (mg CaCO3/L) TR chlorine (mg/L) Temperature (C) 25.0 zq, 2j7q Initial Fal Initial Final I Initial II Final

Environmental Testin-c, Solutions, Inc. Page 6 of 6 Species: Pimephalespromelas Date: /' (10.Dt Client: TVA / Sequoyah Nuclear Plant - Non-treated 3 4 I5 6 I Analyst 74~. Mr:14 - 01.I 1k'-11 ~ ~ I4-Pl'P.4 IiýEk ~ kN Concen-Parameter tration CONTROL pH (S.U.) . *a DO (mg/L) Conductivity C mhoslcrn) 500 Alkalinity (mg CaCO3/L) Hardness (mg CaCO31L) C-Temperature('C) 1,k.R I 2V4.S 7A.] Z.A -t [ Zq. a 2R.Z.7 7 t.(.0 pH (S.U.) 43 1 41 1-944 3 ),Oo ?.2 q36 q;p I 11.3% DO (mgfL) Conductivity ( mhos/cm) At 20 Temperature(0 C) (. .Q,1 21k.t-9 v. S ,S1 -. I-pH (S.U.) ) 7 22.6% DO(rn/L6. Conductivity I L(mhos/cm) Temperature ('C) 2ALA .A 1-S-ZR, 2.- PH(S. U.) I Q15 14-o? 45.2% DO (mg/L) q.2 (o~q Conductivity (4mhos/cm) AtO 0 96 2-Temperature(,C)

q.

2Lb 1M. z--1 14q,9 ,q1 -1A.1 -1 .L[a 72.6% DO (mrnL) 0,0 11 11 e) .r_ Conductivity I( mhos/cm) A8As0 R9 Temperature (QC) .4 2qL[ 2q.,8 14.. (. "t1. 9 i-.q. 1 PH (S.U.) -ý,qA I q.3 6 1.4q 7 32-Conductivity,1. Tern erature (0 C) 2 q¢ qq ?4*z.( v,4.q 2Q -1q I i nae100% DO (mg/L) 1 1 IVhs/m Inta Alkalinity (mg CaCO3/L) WO Hardness (mg CaCO 3/L) TR chlorine (mg) 11111M.4 -11 Temperature('C) I Z. 2I q,1 1 -, z4.9 q.s I -.7 Z. Initial Final Initial Final Initial Final Initial Final

Environmental Testing Solutions, Inc. Page I of 7 Environmental Testing Solutions, Inc. Page 1 ol<' Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequoyah Nuclear Plant NPDES 0: TN 0026450 Project #: 7_ot County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 .3S c 1130 1815 2500 Diluent volume (mL) 2217.5 2 -24S-1370 685 0 Total volume (mL) 2500 2500 2500 2500 2500 Test or~eanism information: Test information: Orcanism ace: Randomizing template: Date and times organisms 1% "Z'-o6 %118 -ro 7101. Incubator number and were born between: shelf location: Z Pit,;7-Organism Source Cups: *. 6 8.' 1. YCT batch: Culture board: il-2zi-ots. 8,, to - Ir, Transfer bowl information: pH = "1,1 Temperature= "14.! Selenastrum batch: I-Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0 29-O1. 1042._ 6-. A 0 2 Ri-'5-0(. oof I.- v.-Ot. 0t 0 t.,0"I6t 3 I.- o u,-0 0 4 s, (., I k -z.*,* -, a 1. k, I\\ 7.*.-" 1, 4 O Ri

4 IZ-o0.-06 04145 ili-26-0to e) 0620.t 01 -4,0_ -L 5

rL- 0~~t S-1 0 T - L

7. -o-Lz o-o(. tA o*,Zol.o% -4, 07-q[

6 12-N4 -o6 0 4Sl I 1 .&-ll-0

6) 06* 0,

f".O L 0 A 1 7 1

11. 0'o-0*

0951* Control information: 1 2 Acceptance criteria Summary of test endpoints: % of Male Adults: ol. 07_ 20% 7-day LC50 > loo?% 0 Adults having 3,d Broods: /o07' 1O0) -7 80% NOEC 10/67' % Mortality: 0 -7 20% LOEC _>_to0__. Mean Offspring/Female: 29, 2. (60_ > 15.0 offspring/female ChV ) /o.X7. %CV: <..0%' <40.0% 1C25 > OO/7.

Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: A-d -2A.-oý CONTROL - 1 Survival and Reproduction Data Replicate number Day_ 1 __2 3 4 5 6 7 _8 9 10 1 Young produced Q c 0 Q 0-C _____ t__,__ Adult mortality \\_. Young produced 0 o o o o J o] c )a I Adult mortality '.H - 1 K.1'-... 3 Young produced o.._ _0 _ C Adult Mortality K K. 1* K L Io 3*d Brod 4 Yugproduced 14= _k a,__ t Adult mortality D I -ead Young produced c_ __ce __a_ Adult mortality I ý I 6 Young produced L_ 12 to I C_ _c__ _0 la Adult mortality L 7 Young produced I,' il. tA - 'i-A I %A I

?-

IS Total young produced 2% " 2.1 3.1 qlj 29 30 Final Adult Mortality \\,. Xfor 3,d Broods 52 -*< ZT> >e< Note: Adult mortality (L l ive, D =dead) I% Mortality: F Mean Offspring/Female: '2L' 2 CONC: 11.3% Survival and Reproduction Data _______Replicate number____ Day__ 2 .3 1 4 5 6 7 9 9 10 I Young produced (z C) C) 0 Adult mortality I ] ~JY 2 Young produced=0 0 C ) C DC Adl otltYoung produced I ]0

0) 0f

~ l j~ Adult mortality I - Young produced=L LA a) -3 L tI ___Adult mortalityJ il'1 1CJ jL C 1.L+/- 5 ~Young produced C IO -l _I L2 { Adult mortality F - ~. O \\ L j i j ,\\ I' 6 Young produced 01 L[---i'1' 7 Young produced I -" 112 1-1U q a) 1 t V Iý Total young produced -Z R~ 32-Z. 2,i a 3 Z 33 28 29 2-9 Final Adult Mortality \\,' \\. \\..-. T1 \\.- I, t-_Z I Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: "/ I Mean Offspring/Female: I 2AA I I% Reduction from Control: -'. 3%" 1

Environmental Testing Solutions, Inc. Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date: V\\-k -Oko CONc: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced C C)o C0 c,) Adult mortality Young produced [U c_ __jt@ [°{j3I{ 3 Aon rduced C)Q Adult mortality 4 Young produced is 14 LA_ I Adult mortality L Young produced C o L._I I c c Adult mortali ty____JOI LK2LIOL t__ U1 U K_ 6 Young produced 1L I T) Z Adult mortality I ýj L-j I ~ ~J~~__L Young produced I tO I.. \\'5 I Lk t~ '11-is l1 Total young produced 2 "A 3 C2 \\ 3_ Final Adult Mortality \\ k .. [ U L-U-Note: Adult monality (L = live, 3 = dead) Concentration: % Mortality: j Mean Offspring/Female: 30. % Reduction from Control: "-1,1'. j CONC: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 Young produced 0 c C 0 c) 010 Adult mortality H. K 2 Young produced: _C_)_ 3 Young produced C) Adult mortality 'L. Young produced LI LA LI__k IS Adult mortality k c-. 5 [Young produced kol ~ c ~ j~~~ cCc Adult mortality IU ._1__. 6d lm raiy t.... I _ a 1 ' ~ I a i T l3 Young produced jCOf_ _ Il 7 1 Youngproduced L*, I' 1-1 [t 4 I. * -. 1*5 L -1 Total young produced 31* 32-31\\ 32 Zi 2S 850 30 -3 1 71 Final Adult Mortality L C Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 07, I Mean Offspring/Female:

30. 4 1

I Reduction from Control: I - -1. 97,,

Environmental Testing Solutions, Inc. Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date: t\\-,.2e.-oi CONc: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced o 0 0

0.

0 0 Adult mortality ,_.-L 2 Yug produced C I*.* q q C-1 cl O-1 CD 0 Young produced o 0 0 )l c 10 Adult mortality, 4 Young produced iA L kAJ sj Lt c[.SL f 9 L 6 Young produced 0 0 0, j0\\ C) tO TCI 0 Total young produced 3iq 51

3l 3*

2

Final Adult Mortality J '_

Note: Adult mortality (L live, D dead) Concentration: % Mortality: o7, Mean Offspring/Female: ,3 1 A % Reduction from Control: -l. t. J'7 CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced C) c) 0 C 0 C 0 Adult mortality Adult mortality _E Ofo Y oung produced. Adult mortality { 6 [ Young produced d (2] S Young produced C) Q ~ ~ e i ng 3rod__ced0 1_ 31 7 Ad Young produced Lt! Ito 15 5 1, e I Ll 1 -1 I1 Total young produced "( -3 )

o 79

2.

[ 347-- Final Adult Mortality' C,. C,_ k._ Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 07. Mean Offspring/Female: 31. % Reduction from Control: - 13. t7.

Environmental Testing Solutions, Inc. Page 5 of 7 Species: Ceriodaphnia dubia Client: Seciuovah Nuclear Plant - Non-treated Date: 1\\-2.-ot CONTROL -2 Survival and Reproduction Data I Replicate number Day 1 2 3 1 4 5 6 7 8 1 9 10 Young produced 0 0 C) 0 1 O O Adult mortality [ . F._ S K._.- F8 I °ugr~ued C) C) 0I* [* [ d ]( ]( Adult mortality 3 Young produced c) cf Adult mortality j .T_ 4 Young produced [ {_ ) I Adult mortality f J_.. kj L-k-1 O _T 65 Young produced Q____ "Adult mortality

24__

7 Y oungproduced. \\'*

7..

\\'2_

t, Tq ~L\\
k,

\\ i Totalyoung produced "2 2t3 ( c "2 30 ZS z& z-Final Adult Mortality k_. Lk \\.- L. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 6 7. Mean Offs ingfFemale:

21. 6 CONC: 100% Intake Survival and Reproduction Data Replicate number Day 1

2 3 4 s 6 7 8 9 10 I Young produced C-Q 0 0 0 0 0 (_ 1_ Adult mortality k.1_. I t - L 2 Young produced (0 ) C 3 JYoung produced i(c} _ Adult mortality j Adult mortality 5 ] Young produced 12-1 ."_I* tZ l7-I Adult mortality Ul_._ [,..! d K 6 Young produced 0~3 i Adult mortality L jL LI I 4 t Totalyoung produced

32.

2& B I z2 i ZR 32 30 so Final Adult Mortality L-C Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 017. Mean Offspring/Female: 30.4 % Reduction from Control: _ o. L *7.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Verification of Ceriodaphnia Reproduction Totals Control-i ReDa pchte number Day 2 2 3 4 5 7 8 9F l Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 4 4 4 4 3 4 3 3 4 36 5 0 0 0 0 0 0 0 0 0 0 0 6 9 11 12 10 10 10 12 10 13 11 108 7 13 12 15 14 14 14 12 16 13 15 138 Total 25 27 31 28 1 28 27 28 229 9 30 282 11.3% Day Rep lcate nunmber D 1 2 3 4 5 6 7 8 9 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 3 5 5 6 4 4 4 4 43 5 0 0 0 0 0 0 0 0 0 0 0 6 9 12 12 10 10 11 13 10 11 11 109 7 13 12 17 14 13 15 16 14 14 14 142 Total 26 28 32 29 28 32 33 28 29 29 294 22.6% Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 4 4 5 4 4 6 4 4 5 43 5 0 0 0 0 0 0 0 0 0 0 0 6 10 12 10 13 11 11 11 12 10 12 112 7 16 14 15 14 14 16 13 15 17 14 148 Total 29 3029 32 29 31 30 31 31 31 303 45.2% Day Replicate number Total 1 1 2 3 4 5 6 7 8 9 10 I o 0 0 0 0 0 0 0 0 0 o 0o 2 0 0 0 0 0-0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 4 6 4 4 5 45 S 0 14 0 11 0 0 0 0 0 0 25 6 11 0 13 0 10 10 12 11 13 10 90 7 16 14 13 17 14 14 12 15 15 14 144 Total 31 32 31 32 29 28 30 30 32 29 304 72.6% Replicate number Total Day 1 2 3 4 1 5 6 7 8 9 Tt , 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 6 4 4 6 5 5 4 4 47 5 11 0 0 0 0 12 0 13 11 0 47 6 0 13 11 12 10 0 11 0 0 11 68 S1 73 14 14 14 16 15 18 15 13 149 Total 28 35 31 30 28 34 31 36 30 28 311 100% Day Re licate nuntie Total Dy 2 13 4 15 6-7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 5 4 6 5 6 4 5 48 5 0 12 0 0 0 0 0 0 0 13 25. 6 12 0 10 13 11 12 11 14 10 0 93 7 14 15 16 15 15 8 17 15 14 153 Total 31 31 30 33 30 36 30 37 29 32 319 Control-2 Day Replicate number To-1 2 3 4 5 6 7 8 1 -9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 05 0 0 0 0 4 4 3 3 3 4 3 5 0 4 3 36 5 11 0 0 0 0 0 0 0 0 11 22 "" 6 0 10 10 9 12 10" 9 11 10 0 81

7 13 12 12 16 14 13 16 13 14 14 137 Total 28 25 25 28 30 26 30 28 28 28 276 100% Intake Day l~e R licate number 1

2 3 4 5 6 7 1 8 9 1 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 3 4 4 5 41 5 13 0 12 12 0 11 13 12 12 0 85 6 0 it 0 0 12 0 0 0 0 to 33 7 15 13 14 17 13 13 16 14 15 15 145 Total 32 28 31 33 29 28 32 30 31 30 304

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2906 Reveiwed by: 4 < Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) pooled controls (%) Control - 1 25 27 31 28 28 27 28 29 29 30 100 28.2 6.0 Not applicable 11.3% 26 28 32 29 28 32 33 28 29 29 100 29.4 7.6 -4.3 22.6% 29 30 29 32 29 31 30 31 31 31 100 30.3 3.5 -7.4 45.2% 31 32 31 32 29 28 30 30 32 29 100 30.4 4.7 -7.8 72.6% 28 35 3i 30 28 34 31 36 30 28 100 31.1 9.5 -10.3 100% 31 31 30 33 30 36 30 37 29 32 100 31.9 8.4 -13.1 Control - 2 28 25 25 28 30 26 30 28 28 28 100 27.6 6.4 Not applicable 100% Intake 32 28 31 33 29 28 32 30 31 30 100 30.4 5.6 -10.1 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MNISD value: PMSD: 2.166 7.7 1.353 4.9 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA (10th percentile) 1%. Upper PMSD bound determined by USEPA (90", percentile) 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Staue Date: [1128/2006 Test ID: CdFRCR Sample ID: TV'A/ SequoyahNuclear Plait, Outfall 101 Dnd Date: 12/5/2006 Lab ID, ETS-Ensv. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Contlol 25.000 27000 31 000 28.000 28.000 27.000 28.000 29.000 29.000 30.000 11.3 26000 28.000 32.000 29.000 28.000 32.000 33.000 28.000 29.000 29.000 22,6 29.000 30.000 29.000 32.000 29000 31.000 30.000 31.000 31.000 31.000 45.2 318000 32.000 31.000 32.000 29.000 28.000 30.000 30.000 .32.000 29.000 72.6 28.000 35.000 31.000 30.000 28.000 34.000 31.000 36.000 30.000 28.000 100 31.000 31.000 30.000 33.000 30.000 36.0M0 30.000 37.000 29.000 3.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N f-Stat Critical MSD Mean N-Mean D-Control 28.200 1.0000 28,200 25.000 31.000 5.981 10 30.217 1.0000 11.3 29,400 1.0426 29.400 26.000 33.000 7.555 10 -1.267 2.287 2,166 30,217 1.0000 22.6 30,300 1.0745 30.300 29.000 32.000 3.496 10 -2.217 2.287 2.166 30217 1.0000 45.2 30400 1.0780 30.400 28.000 32000 4.703 10 -2.322 2.287 2.166 30.217 1.0000 72.6 31.100 1.1028 31.100 28.000 36.000 9.520 10 -3.061 2.287 2.166 30.217 1.0000 100 31.900 1 1312 31900 29.000 37.000 8.418 10 -3.906 2.287 2.166 30.217 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kohnogorov D Test indicates normaldistribution (p > 0.01) 0.938959122 1.035 0.601190678 0.062444916 Bartlett's Test indicates equal variances (p 0.04) 11.9811039 15.08627224 Hlypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dtannett's Test 100 >100 1 2.16619565 0.076815449 16.77666667 4.487037037 0.00559108 5,54 Treatmrents vs D-Control Linear Interpolation (200 Resampfes) Point SD 95% CL Skew IC05 >100 ICIO >100 IC15 >100 1.0 IC20 >100 0.9 1C25 >100 0. IC40 >100 07 ICSO >100 0.6 O 0.5 o 0.4 0.3 0.2 0.1 0o.0 -0.1 -0.2 0 20 40 60 80 100 120 Dose % Dose-Response Plot 40-35 t-tail, 0.05 levet t25 of significance 20 o 0e i~s 5 -6 .sqn_ 1]-28-O6data

TVA / Sequoyah Nuclear Plant, Intake Non-treated November 28 - December 05, 2006 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/28/2006 Test ID: CdFRCR Sample ID: TVA/ Sequoyah Nuclear Plant, Intake End Date: 12/5/2006 Lab ID: ETS.Envir. Testing Sol Sample Type: DMR-Discharge MonitoringReport Sample Date: Protocol FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments: Non-neated Cone-%!0 1 2 3 4 5 6 7 8 9 10 D-Control 28.000 25.000 25.000 28.000 30.000 26.000 30.000 28.000 28.000 28.000 100 32.000 28.000 31.000 33.000 29.000 28.000 32,000 30.000 31,000 30,000 Transormn: Untransformed I-Tailed Isotonic Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 27.600 1.0000 27.600 25,000 30.000 6.436 10 29.000 1.0000 100 30.400 1.1014 30.400 28.000 33.000 5.634 10 -3.588 1.734 1.353 29.000 10000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal ditribution (p > 0.01) 0.919082224 0.868 -0.18624647 -0.98033966 F-Test indicates equal variances qp = 0.92) 1.075757623 6.541089535 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F.Prob df HomoscedastictTestindicates nosigridicantdifferences 1.353112957 0.049025832 39.2 3.044444444 0.002101165 1,13 Treatments vs D-Cont!ol Linear Interpolation (200 Resamples) Point SD 95% CL Skew IC05 >100 IC1O >100 ICI5 >100 1.0-IC20 >100 0.9 IC25 >100 0.8 IC40 >100 0.7 ICS0 >100 0.7 0.5 & 0.4-0.3 0.2 0.1 0.0 -0.1 -0.2 0 20 40 60 .*0 too 120 Dose % Dose-Response Plot 35 30 25 0 -15 10 5. I -tail, 0.05 level of significance Y0 xqnrl-28-O6data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses Project number: 2906 Reviewed by: Concentration Parameter Dai 0 Day 1 Day 2 Dai 3 Day 4 Day 5 Da 6 _Initial Final Initial I Final Initial I Final Initial Final Initial Final Initial Final Initial Final PH (SU) 7.69 7.35 7.751 7.21 7.591 7.27 7.82 7.36 7.89 7.50 7.90 7.34 7.65 7.50 DO (mg/L) 7.7 7.8 7-8 7.5 7.7 7.71 76 7.7 7.7 7.8 7.8 7.8 7.8 7.9 Conductivit~y ([timbos/cm) 318 318 315= 37 30Y00.0 Control Alkalinity (mg/L CaCO 3) 5 5 58 Hardness (mgIL CaCO3) 92 86 90 Temperature (C) 24.7 25.1 24.7 24.7 24.6 24.9 24.8 25.2 24.71 24.7 24.8 24.8 24.6 24.8 pH (SU) 8.00 7.35 7.43 7.20 7.28 7.26 7.33 7.35 7.44 7.49 7.66 7.34 7.38 7.43 DO (mg/L) . 7.7 7.8 7.8 7.5 7.6 7.7 7.8 7.8 7.8 7.8 8.1 7.8 8.1 7.9 11.3% Conductivity (Itmhos/cm) 301 286 284 290 Temperature (0C) 24.7 24.8 24.8 24.7 24.8 24.6 24.8 25.0 24.7 25.0 24.8 24.7 24.6 24.6 PH (SU) 8.00 7.35 7.42 7.20 7.27 7.27 7.32 7.35 7.43 7.50 7.641 7.33 7.38 7.44 22.6% DO (mg/L) 7.7 7.8 7.8 7.5 7.6 7.8 7.7 7.81 7.8 8.11 7.7 8.1 7. Conductivity ([&mhos/cm) 287 289 282 275 27,5, 273 277 Temperature (OC) 24.9 24.8 24.8 24.7 24.8 24.8 24.9 24.9 24.7 25.0 24.9 24.8 24.7 24.6 pH (SU) 8.01 7.35 7.40 7.21 7.25 7.25 7.29 7.34 7.43 7.51 7.58 7.32 7.36 7.45 DO (mg/L) 7.7 7.8 7.8 7.4 7.6 7.6 7.9 7.7 7.8 7.8 8.2 7.7 8.1 7.9 45'2"% Conductivity (O.mhos/cm) 260 26 256 260 2481 252 Temperature (,C) 24.9 24.8 24.8 24.9 24.9 24.7 24.9 25.1 24.7 25.1 24.9 24.8 24.7 24.5 pH (SU) 7.99 7.34 7.39 7.20 7.23 7.26 7.27 7.34 7.38 7.49 7.56 7.35 7.35 7.45 DO (mg/L) 7.7 7.9 7.7 7.3 7.8 7.5 8.0 7.7 7.8 7.8 8.2 7.7 8.1 7.9 72.6% Conductivity (timhos/cm) 234 225 222 218 215 218 222 Temperature (°C) 25.0 24.8 . 24.9 24.6 24.9 24.7 24.9 24.9 24.8 24 .9.9 24.8 24.7 24.7 P S)7.97 7.36 7.401 7.221 7.201 7.26 7.221 7.331 7.38 79 7.49 J 7.35 7.321 7.451 DO________________7.77 7.9, 7.71 7.2 7.91 7.5, 8.21 7.61 8.0 778.21 7.7 8.11 7.81 Conductivity (pmhosfcm) 189 190 1 185 8 189 9 100% Alkalinity (mg"L CaCO,) 66 66 66 Hardness (mg/L CaCOj) 7 86 Total Residual Chlorine (m_(L) <0.10 <0.10 <0 Temperature (C) 25.2 24.9 24.9 24.6 25.1 24.81 25.0 250 24.8 24.9 24.9 24.9 24.71 24.6 PH (SU) 7.951 7-351 7.391 7.21 7.171 7.231 7.20 7.41 7321 7.501 7.441 7.331 7.321 7.441 DO (mg/L) 7.7 8.1 7.91 7.6 7.91 7.61. 8.2 788.01 7.81 8.21 7.81 8.2 J 7.8 Conductivity mhos/cm19 191 184 18 100% Intake Alkalinity (mg/L CaCO3) 65 66" 6 Hardness (mg/L CaCO3) 80 84 8 Total Residual Chlorine (mg/L) <0.0 <0.10 <0. _Temperature (°C) 25.1 24.71 24.7 24.8 25.0 24.7 24.9 25.0 24.8 24.8 24.9 24.7 24.61 24.9

Environmental Testing Solutions, lDc. Page 6 of 7 m EniomntlTsin Souios n.Pge6o Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Daily Chenjz :. Date: ý \\-l 7-0k,, D a y Concentration Parameter CONTROL pH (S.U.) 1 DO (mg/L) Conductivity (pamhos/cm) Alkalinity (mg CaCO 3/L) Hardness (mg CaCO3/L) Temperature (°C) v4. 115. t ,-1 214.1 " 6 "N pH (S..U.) 1-14 .3s 7-1.q3 -9q.20 4.28 I 9*, 11.3% DO (mg/L) Conductivity 30 1 (Pmhos/cm) 30 1 3(A Temperature (C) "tk.- I -.q.,& 2 '4 pH(S.U.) 60.oo 2.35-20 22.6% DO (mg/L) ca Conductivity (jimhos/cm) Temperature (C) "N./ 0i L. jI "LA.' -I

A.*-__

I p H(S. U.) t3- 0

I

[ "q3 I .q° ý.Z 7 I . y c, 5 45.2% DO (mg/L) q-E3 Conductivity C) (Oimhos/cm) q A61P Temperature ('C) 7'4A -_I_1._f_______'i.- pH (S.U.)

g.

./3-7 ,z42 72.6% DO (mg/L), Conductivity (Omhos/cm) P* Temperature (T) 7.0 Lq 91i. q j PH (. U40-q2z '4. ZD co 100% DO (mg/L) Alkalinityl (mg CaCO3/L) (.rg CaCO3/L) £ TR chlorine (mg/L)

.Og~*

Temperature (C) ZS*J. l M. I 9-

24.

7 1 7-1 pH ýs.u.) q.o t - I ",35P 2, l ] 9 ? 100% Intake DO (mg/L) -,p q 9 Conductivity (.pmhos/cm)1, q ZI Alkalinity ('mg CaCO 3/L) Hardness (g)TR chlorine <,0i* Temperature(TC) 21,° 1, 2q.-) i -2q,7 : 4z I

o 1 z.

Initial Final I Initial 1 Final Initial Final I

Environmental Testing Solutions, Inc. Page?- of 7 m Species: Ceriodaphnia dubia Client: Seciuovah Nuclear Plant - Non-treated Date: ý\\ -a z.(p Day _3 4 56 Analyst ,4_v( OV-- Concen-Parameter .,o, tration CONTROL [pH (S.U.) '4 2- .ef45 DO (rngIL)- Conductivity (4mhos/om) .30ý 2)03* ,3q Alkalinity X (Ag CaCO3IL) Hardness (mg CaCO 3/L) Temperature (°C) 2.. 2-., .o 2-, pH (S.U.) q~ r)- C)i (aA

14.

3~- q 6 Q4 11.3% DO (mg/L) 66,1 Conductivity O (*imhos/cm) v.Temperature('C) 1 2S. VA - 6 -LA. pH (s.u)

7.

2-q.,, .*3. 9,Nq 22.6% DO (mg/L) e L e,, Conductivity. (JIMhoslcm)9q Temperature ('C) 7z. 7_-k 'A. -0.1 -ZIA R "VA_ I pH(S.U.) "4.2q 2-fP -4 32- _S_ 45.2% DO (mg/L) Conductivity (pmhos/cm) Temperature ('C) 24 .s. 1 '-L". q 1 PH (S.U.) 3_Tq_36_ 72.6% DO (mgIL)eo Conductivity (Aimhos/cm) A Temperature (0 Q -A.'At 2., &.R 1L4. _VA. - ~ R 100% DO (mgL) .2*, (mg CaCO3/L) (i Hardness (mg CaCO3iL) so0 TR Chlorine (mg/L) /O.-1 Temperature) ".O (7-CI,) "'4.& R 2, 11 I.5 q n i-I (9 ! [ Iý 9D 9.J4(

.~

j z 'tiý .6b Q3 Iý qs 9..32 H (S U) 100% Intake DO (mgL) 2-(mg C0TR chlorine (m... Temperature(C i.q Otq 2q.7 I ( n' ýC)M"1 Im

III l

Initial Final IInitial Final Initial Final

Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #:

zkt, Countv: Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information:

Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 S-"39/ 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1370 685 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 21.1e, -M 2

s. ko'-,

O t, Randomizing template: lýue, Date and times organisms tALtj 0 t7,:16O0 Incubator number: were born between: Organism source: A6S AjX 1\\- C'6 ek. Arremia lot number: e*-120,4 Transfer bowl information: pH1= Temperature °C Total drying time: zq-Po,.!ý "1._ _ 7A_. _L Date / Time in: O,, \\L, Average transfer volume: Date / Time out: It-Z-cd,, kL'S Oven temperature: Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 1 .t%,41 I 1-16i-v06A 0t1.,0 4*,L 2I. l].3O.oL' 0* tS o qS2.

\\2t,-Oo,&

Ol2g 0 *0-4I -o,.ot, CABO L56 LA S 0 k*- 6 061 wo\\ W,* AL-4 O7.) 0500 I541$5O I--O2-o16N, 02OX.0 IZ O \\k4 6.. -A 7 Control information: Acceptance criteria Summary of test endpoints: % Mortality: o"'o <20% 7-day LC50 . 1ic 7. Average weight per initial larvae: O. *, NOEC l307. Average weight per surviving larvae: o." 1 0.25 mg/larvae LOEC > 1c607 ChV IC25 > 106 -

Environmental Testing Solutions, Inc. Page 2-of 6 Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimeph ales promnelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: H " a'L)& Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L L0 / L) /0 IC) IC) /0 / U o I.) I 10 I /0 1 )* I) 0 /0 /0 /0 t0 D 0 /C to 0C 2 /010 /o o /0/ /0 /0/0 C/0 / /0 3/ /0 (0 I ./0 /0 / 0 /0 16 /0 /6 0 0 .4 iD O 0 1 O CO 10 10 Co 105 I0 /0 5 /0 C /C) t / ( 0 /0 /C) I I C /0 /0 6 100 0 /O1 I0 (U(C /0 /0 /0 to to 7 iaJo o c)/..C /0 10 '6 1C) IC) /C) t0 /0 A Pan weight (mg) Color identification: zlu~rkf a_ +Y"

1. 10 139 13ti/

1q3 iq.(Cb q.,7( I14.09 l 1!31 NA{,l* q.qO M-9.f3 RqS) B = Pan + Larvae weight a (mg) Analyst: 0*6*" @04* .4 1.8 aoqq 4G-.o a;).lq K0."laa,6.7 @10-11 9; 0-q

113 W-lto Larvae weight (mg) = A - B 53' LA.S

[AA .Ca3 a *~ bto l..SA si1

0.oz,..

t ,a ".o' Weight per initial number of larvae (mg) = C / Initial number of larvae 4o 3' No Na Na 0' V ~0 0 Average Percent weight per reduction initial from control

0. (a(ot number of

(%) larvae (mg) o.*1%~~~Z -Z 142", ,*'5***.*7 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r decanted and returned, w wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Pace 3 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: Survival and Growth Data Day 45.2% 72.6% 100% _M N 0 P Q R S T U V W X 10 /U ) IC o /L)J 10 jo 0o Io JO /0 t /0 I

10) 0t

ýD I* C) 10 10 1 16 10 10 1O IO 2 1G 0 1 C) 10 ID to IO) to / I0 10to o t o / 0 t o 1 / 0 1 0 ( 0 ' 00 t 0 1 0 / 0 4 0 (1 (0 10 10 I 0 /0 /t /0 /0 /0 5/ 10 /0 (0 /C (0 (0 /0 /0 10 10 /0 6 /0 /0 /0 (/O /0 /0 0 (Q 1 /0 /0 /0 /0 /0 /0 (0 /0 10 /0 (0 /0 A Pan weight (mg) Color identification: Analyst: J-FgB B = Pan + Larvae weight (rag) 3o.aq ao.ta t7.83 a0.33 ivo.5 ao lit a a. 3 -ao.ol al.oi a,.'- 6 -Ra.ri ao. xif Analyst: L143[ Larvae weight (mg) = A - B to, -1., uo'A b* (,,07. SAIl <S.A5 6-5t.2.*,.0

6 "11 1-S T49g*,z Weight per initial number of larvae (mg)

A = C / Initial number of larvae Average Percent weight per reduction initial from control -Z number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm= unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: 'l6.co Survival and Growth Data Day 100% Intake Y z Y Z AA BB 0 10 /o /0 1o 1 10 10 /1 D 0 2 (U /0 10 '0 3 icG /0 /0 /0 4 /0 10 /0 ,0 60 (0 D 0 /0 6 7 c D o 10 A = Pan weight (mg) Color identification: _mq jt Iq.q lToB Iq Y.(06 1L33 Analyst:" _F B = Pan + Larvae weight (rag) Analyst: {*g S[ al.iý ac-.\\o ai,33 a1.1s Larvae weight (mg) = A - B Weight per initial number of larvae (mg) 01% C/Initial number of larvae %0 \\D (" I 0 Average Percent weight per reduction initial from control

0. t~' 4 2.

7 number of (%) I larvae ýmg) Comment codes: c'= clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm= unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: 4-Comments:

TVA / Sequoyali Nuclear Plant, Outfall 101 UV-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwed by: I ( ] Project number: 2906 Not for Compliance Assessment, Internal Laboratory QC I..._"_ Concentraeion 5/) Replicate Inital numrber of Final number of l.ae-A - Pan weight B =Pan + Larvae Larvue weight (ng) Weight I Survying Mean Weight / Coeffcient of a-iation Weight I Initial number Mean snrvlou Mean weight I CoeiMcent of Percent reduction from lirae (mg&) eight (rg) A-B number of larv2e (mg) Surviving number of 011-a -u thilO P11 t1,g of larvae (mag) (%) Initial number of variatlon t(t..-o*-gh control (%) larvae (mg) P.,ur*I .0..) 5%) larvae (rmg) ee aItoonob ot ho..) A 10 10 14.67 20.32 5.65 0.565 0.565 Control B 10 10 13.99 20.44 6+45 0.645 0.661 12.3 0.645 100.0 0.661 12.3 Not applicable C 10 to 13.49 20.18 6.69 0.669 0.669 D 10 10 14.31 21.94 7.63 0.763 0.763 E 10 10 14.60 21.05 6-45 0.645 0.645 11.3Y. F 10 10 14.76 22.14 7.38 0.738 0.675 6.3 0.738 100.0 0.675 6.3 -2.2 G 10 10 14.09 20.70 6.61 0.661 0.661 H 10 10 16.31 22.87 6.56 0.656 0.656 1 10 10 14.14 20.11 5.97 0.597 0.597 22.6/4 J 10 10 14.70 20.72 6.02 0,602 0.635 0.2 0.602 100.0 0.635 0.2 3.9 K 10 10 14.83 21.13 6.30 0.630 0.630 L 10 10 14.51 21.60 7.09 0.709 0.709 1 M 10 10 13.90 20.24 6.34 0.634 0.634 45.2 N 10 to 14.78 20.82 6.04 0.604 0.588 0.9 0.604 100.0 0.588 8.9 10.9 o to 10 14.70 19.83 5.13 0.513 0.513 P 10 10 14.31 20.33 6.02 0.602 0.602 10 10 14.23 19.65 5.42 0.542 0.542 72.6% R 10 t0 14.98 20.81 5.03 0.583 0.585 6.2 0.583 100.0 0.585 6.2 11.5 S 10 10 14.99 20.82 5.83 0.583 0.583 T 10 10 14.31 20.61 6.30 0.630 0.630 U 10 10 14.64 21.01 6.37 0.637 0.637 1001/ V to 10 14.71 21.76 7.05 0.705 0.678 8.7 0.705 100.0 0.678 8.7 -2.7 W 10. 10 15.23 22.71 7.48 0.748 0.748 X 10 10 14.26 20.49 6.23 0.623 0.623 Y 10 10 14.95 21.14 6.19 0.619 0.619 100'. Intake Z 10 10 14.08 20.16 6.08 0.608 0.644 5.6 0.608 100.0 0.644 5.6 2.5 AA t0 10 14.66 21.33 6.67 0.667 0.667 I s 10 10 14.33 21.15 6.82 0.682 0.682 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: MSD = PMSD: 0.0949 14.4 0.0866 13.1 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. Oil average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.5% fiom the control (determined through. reference toxicant testing). Lower PMSD bound determined by USEPA (10th percentile) 9.4%. Upper PMSD bound determined by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Envirotmental Protection Agency, Cincinnati, OH.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 28 - December 05, 2006 Statistical Analyses Larcval Fish Growth and Survival Test-7 Day Growth Start Date: 11128/2006 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plart, Outfall 101 End Date: 1215/2006 Lab ID: ETS-Envir. Testing Sol. Sample 'ype: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCI1R-EPA-821--R-02-013 Test Species: PP-Pimephales promelas Commsoonenrts: UIV-treated Conc-% 1 2 3 4 D-Conlrol 0,5650 0,6450 06690 0.7630 11.3 0.6450 0.7380 0.6610 0.6560 22.6 0.5970 0.6020 0.6300 0.7090 45.2 0.6340 0.6040 0.5130 0.6020 72.6 0.5420 0,5830 0.5830 0.6300 100 0.6370 0.7050 0.7480 0,6230 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mea n Mi Max CV% N tI-tat Critical MSD Mean N-Mean D-Control 0.6605 1.0000 0.6605 05650 0.7630 12.343 4 0,6678 1.0000 11.3 0.6750 1.0220 0.6750 0.6450 0.7380 6.301 4 -0.368 2.410 0.0949 0.6678 1.0000 22.6 0.6345 0.9606 0.6345 0.5970 0.7090 8.155 4 0.660 2.410 0.0949 0.6345 0.9502 45.2 0.5883 0.8906 0.5883 0.5130 0.6340 8.884 4 1.835 2.410 0.0949 0.6170 0.9240 72.6 0.5045 0.8849 05843 0.5420 0.6300 6.154 4 1,930 2.410 0.0949 0.6170 0,9240 100 0.6783 1.0269 0.6783 0.6230 0.7480 8.654 4 -0.451 2.410 0.0949 0.6170 0.9240 Auxiliary Tests Statistic Critical Skew Kurt Shiapiro-Will/s Test indicates normal distribution (p > 0.01) 0.98408103 0.884 0.220567588 -0.32046338 Bartlett's Test indicates equal variances (p = 0.82) 2.173702478 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TO MSDu MSDp MSB MSE F-Prob df Duraett's Test 100 >100 1 0.094887324 0.14365994 0.007069367 0.003100361 0.090179361 5, 18 Treatments vs D-Control Lin rInterpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 22.778 ICIO >100 ICI5 >100 1.0 IC20 >100 0. 1C25 >100 IC40 >100 0.8 IC50 >100 0.7 0.6 0.5 o 0.4 0.3 0.2 o1 0.0 -0.1 0 20 40 60 80 100 120 Dose % Dose-Response Plot 0.9 0.0 0.7

0.6 1-tail, 0.05 level a si.f.icance 2 0.5 0.4 0.3 02 0.1

.sqn_ ]1-28-06ddta

TVA / Sequoyah Nuclear Plant, Intake UV-treated November 28 - December 05, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/28/2006 Test ID: PpFR.CR Sample ID: TVA/ Sequoyah Nuclear Plant, Intake End Date: 12/5/2006 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol FWCHR-EPA-821-R-02-013 Test Species: PP-Pinephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5650 06450 0.6690 0.7630 100 0.6190 0.6080 0.6670 0.6820 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.6605 1.0000 0.6605 0.5650 0.7630 12.343 4 0,6605 1.0000 100 0.6440 0.9750 0.6440 0.6080 0.6820 5.594 4 0370 1.943 0.0866 0.6440 0.9750 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-W,*s' Test indicates normal distribution (p > 0.01) 0.98164469 0.749 0.198999913 0.957942952 F-Test indicates equal varianees (p = 0.21) 5.120441914 47.46722794 "yothesis Test (8-tail, 0.05) MSDu MSDp MSB MSE P-Prob df Homoscedastic t Test insdicates no significant differences 0086598793 0.13 1110965 00005445 0.003972167 0.723920584 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 >100 ICIO >100 IC15 >100 i.0 IC20 >100 0.9 IC25 >100 IC40 >100 0.8 IC50 >100 0.7 a 0.6 0.5 S0.4 0.3 0,2 0.1 0.0-------------------------.. 0 20 40 60 80 to0 120 Dose % Dose-Response Plot 0.9 0.8 07 E 0.6 tail, 0.05 level of significance 0 0.5 0.2 0.2 01 0 1E 6 sqrnl l-28-O6dala

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 28 - December 05, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 2906 Reviewed by: Concentration Parameter Da 0 Day I Day 2 Day 3 Day 4 Day 5 Da' 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.91 7.31 7.40 7.11 7.42 7.14 7.25 7.18 7.38 7.15 7.44 7.14 7.37 7.26 DO (mg/L) 7.8 7.8 8.1 7.1 7.7 7.4 7.7 7.7 7.7 7.0 8.0 7.8 8.1 7.6 Control Conductivity (jImhos/cm) 311 307 304 294 295 294 298 Temperature (°C) 24.9 24.7 24.6 24.5 24.8 24.9 24.8 24.6 24.8 24.4 24.9 24.2 24.7 24.5 PH (SU) 7.91 7.28 7.40 7.08 7.41 7.14 7.25 7.16 7.36, 7.15 7.46 7.13 7.37 7.22 1D.30/. DO (mg/L) 7.7 7.9 8.1 6.8 7.7 7.4 7.7 7.5 7.8 7.0 8.0 7.8 8.0 7.6 Conductivity (gtmhos/cm) 305 299 291 290 291 288 291 Temperature (°C) 24.9 24.5 24.7 24.9 24.8 24.9 24.3 24.8 24.3 25.0 24.1 24.7

24. 1 pH (SU) 7.90 7.28 7.39 7.07 7.40 7.13 7.25 7.16 7.36 7.14 7.46 7.13 7.36 7.25 DO (mg/L) 7.7 7.7 8.1 6.9 7.7 7.4 7.8 7.5 7.9 6.9 8.1 7.7 8.0 7.6 22.6%

Conductivity (pimhos/cm) 29 284 .276 276 277 275 279 Temperature ('C) 24.9 24.8 24.8 24.5 24.9 24.8 24.9 24.3 24.8 24.3 24.9 24.3 24.8 24.2 pH (SU) 7.90 7.23 7.40 7.05 7.37 7.13 7.24 7.17 7.34 7.10 7.45 7.13 7.36 7.25 DO (mg/L) 7.8 7.6 8.2 6.8 7.8 7.4 7.8 7.5 7.9 6.9 8.1 7.5 8.0 7.5 45.2% Conductivity (ptmhos/cm) 261 258 254 I_ 263 ___ 254 253 255 Temperature (oC) 25.0 24.6 25.0 24.6 24.9 24.9 24.9 24.4 24.8 24.4 24.9 24.2 24.8 24 3 pH (SU.) 7.90 7.29 7.38 7.06 7.37 7.13 7.20 7.19 7.32 7.15 7.42 7.09 7.33 7.23 72.6% DO (mg/L) 7.8 7.5 8.1 6.7 7.8 7.3 7.9 7.4 7.9 6.8 8.1 7.8 8.0 7.6 Conductivity (uimhos/cm) 230_ 223 _223 220 218 220 224 Temperature (*C) 25.1 24.6 25.0 24.6 25.0 24.9 24.9 24.4 24.8 24.5 24.9 24.2 . 24.8 24.2 pH (SU) 7.92 7.29 7.37 7.06 7.35 7.12 7.19 7.18 7.31 7.10 7.42 7.20 7.34 7.24 100% DO (mg/L) 7.6 7.4 8.1 6.6 .7.8 7.4 7.9 7.5 7.81 6.8 7.9 7.4 8.0 7.6 Conductivity (jjmhos/cm) 194 M92 194 190 91 190M 193 Temperature (°C) 25.1 24.4 25.0 24.6 .25.1 24.9 25.0 24.5 25.0 24.4 25.0 24.2 24.8 24.2 pH (SU) 7.91 7.25 7.38 6.96 7.16 7.12 7.16 7.18 7.28 7.14 7.37 7.15 7.31 7.24 DO (mg/L) 7.6 7.6 8.0 6.7 7.9 7.5 8.0 7.5 8.0 6.9 8.0 7.5 8.1 7.6 Conductivity (prmhos/cm) 195 185_[_87 187 188 187 190 _Temperature ('C) 25.0 24.4 24.9 24.6 24.9 24.9 24.31 24.8 24.5 25.0 24.3 24.8 24.3

Environmental Testing Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: Jiioýe.10 Daily Chemistry: Day 0.Z 24 9 , Ana*t A6A-L-vk4- /gk /l: Concentration Parameter

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Environmental Testincy Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pirnephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: I /.Q .- (o Dav 4 55 Analyst 1'~L l',- Concen-Parameter tration CONTROL pH (S. U.) t" DO (mg/L) Conductivity ~ L c (pmhos/cm) ACW 91q ac l 6 Temperature ('Q) 14... zL- "R2 z4 A,-4-S .11.3% DO (mg/L) Conductivity (prnhos/cm) lo9 I Temperature(C) 14.- A, 13 '. i I 11A pH (S.U.) ls 1L2.33 22.6% DOr.) '.5 S L.I 9.0 Conductivity (ýimhos/cm) L9 I .i Temperature(°C) ('.Q. -Lq.* L.I ZL'.9 2 ?, I pH (S.U.) q- / ?-q5 4/ 45.2% DO (mgL)

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Environmental Testing Solutions, Inc. I" ,Page 314 Page 1 of J Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst I*' Date analyzed ] ,,o Iodide reagent: IJ,2 ' I5" Acid reagent: I W-A 4 -4 Calibration: i:!i}*'*:'.

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I 1.00 Reerenee standard number W.S Note: For samples with a residual chlorine of > 1.0 mg/I!, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/IL) (mg/L) (acceptable range = 90 to 110%) 1 ~ 3-S53 0.50 .fc 16*.6/. Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]) x 100 number (mg/L) (acceptable range= + 10%) Duplicate D j Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) ,;..$.i2.. Blank (should be= <0 10 mg/L) 651{) O 5 a \\2..0 ZtJe rm'-'qttU Ch m tz-)- r) 0cc t OIL. ,O tOooo eo3 0(Jzk, 07-qyc.. ( Ca ,) oa ko/ oo-0005D3 Gt.-\\xn.ol TV-*A -SQI\\- 161 noc0.oc_, k.D*z z.00oo-00 NVote. All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) fý 0-Z .5o0.5I /0 .12 1/. 1 Reviewed by I'A( _.l Date reviewedI e 1 -8,

Environmental Testing Solutions, Inc. -t-4o.8/. Page 3 Page I of Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst '&kl. Date analyzed -L 6 6,0. Iodide reagent: A)A 3S' Acid reagent: -IkL L"'4-F} Calibration: ',2 0.10 mg/L 1.00 mg/L Reference standard number 15sS

5 J,.-555 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS =MV.TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.5 5S o.50 D. q2 6,q Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) SBlank (should be=<0.10 mg/L) /,c)~. ooeo2 -;~.e,-

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J--.II, Environmental Testino Slntiinnv Inc Page J_ Page / of J Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL 0 0. 10 mg/L Meter: Accurnet Model AR25 pH/Ion Meter Analyst IýLt, Date analyzed Iý O2 v Iodide reagent: Z35 Acid reagent: (/ Q Ll -1 Calibration:

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'SS 33 S / Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine °/RPD ="(S - D) /[(S+D)/21} x 100 number (mg/L) (acceptable range = 00/) C) t 1 4-LV40jf lUC,0)~ S 0LO.QJAa2 Duplicate Di .o:...... ,: D Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/t) B lank (should be= <0.10 mg/L) o... 611/-~ 0v tot -2t~ k-o 0 -4 0 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV / TV x 100 number (nag/L) (mg/L) (acceptable range =90 to 110%) ItA's -s ý, 0.50 -46 6.5 (,lo I Reviewed by Date reviewed

Environmental Testing Solutions, Inc. rage ) Page __ of _ Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L 13 Analyst 7 rJ1.. Rd-V53-Date analyzed Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (,N) of H2S0' pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2CO3 x 0.05)/E = (Nx 50000)1100 ml sample water reference standard ml ml ml 0.25/E =Nx 500 4.5 S.U. number number (E) (acceptable range 0.013 - 0.022)

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Environmental Testing Solutions, Inc. Page ..j of Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO 3/L 105.3 Analyst C fl*g. q Date analyzed Titrate samples to pH = 4.50 S.U. Titrant normality and muhriplier determination: pH of Normality Normality (A) of H,S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na 2 CO3 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E =N x 500 4.5 S.U. number number (E) (acceptable range = 0.018 - 0.022) c, -m0aq O~n4 o~ O. 2s- /. 6L cxod-)U-., o.o-o .0o 'o - Laboratory coi-reo! standard: Reference True value Sample Alkalinity (MV) % RS MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (Mg CaCO3/L) (acceptable range (mg CaCOvL) (ml) ml ml ml = 90 to 110%) 100. 100 A, d.3

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Duolicate sam le precision: Sample Alkalinity/ %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /((S+D)/21) x 100 number (ml) ml ml ml (acceptable range = + 10%) )2-z-+* Fr14 .5

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Environmental Testing Solutions, Inc. Vage iu Page 1 of _ Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Time initiated: Date analyzed j-Time completed: Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or 'lultiplier reference standard ml ml ml . = 0.2/E = (N x 50000)/ 50 ml sample number number (E) (acceptable range = 0.018 - 0.022) = N x 1000 O.L) q, e

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Duplicate (3) Jo' aov i]D C1C / Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mg CaCO3[L) (ml) mI ml ml LL30 Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (Mg CaCO3/L) MV= A - B (acceptable range (Mg CaCO3/L) 75 to 125%) O47_ ,It 5 Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) mrl ml ml Multiplier (mg CaCO3/L) Blank J 0 (should be = 0 mg CaCOs/L) ND, 0.0 0,0 0D rn 1 S47-o A. ,5-. L-, 9 2-

1) -;., to1.

o" Note: If >15ml oftitrant is used, sample must be diluted. Reviewed by: I J\\ r Date reviewed

Page -ýA Page ____ of__ Environmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaC03,L Analyst h' i Time initiated: cj 2 1 Date analyzed Time completed: Cq L4"2-Titrant normality and multiplier determination: Titrant . Normality check Begin End Total Normality (A') of EDTA pH Factor or Multiplier reference standard ml ml ml= 0.2/E (N x 50000)/ 50 ml sample number number (E) (acceptable range = 0.018 - 0.022) = Nx 1000 lAJXq6 I M -O(4 D-0 61 9 - )

0. o.

)( Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (Ing CaCO 3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml 90 to 110%) 40 50 9 t3

2. 0 o.i 2

102,5. Matrix soike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L) (mg CaCO 3/L) (ml) ml ml ml E'1 5op) hIo.2 (0.3

20.

30 Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (mg CaCO 3/L) MV = A - B (acceptable range (rag CaCO3[L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) Blank TV:- -- )j ý (should be= 0 mg CaCOu/L) O-O .0 0.C0 Ain V2.oz-.oC. j:0 AA-S 1i _-S aQO .5 1'2. 02.- O C

i.

, 3L -7 I D(., 03J Jý %IU-4 0 (A-5jLPI ý .5§ 36,q qq 9 -0 t1-0-O 2-385' qZeO q1 at. al,.O\\ 0 _i ,LA .6 .-2 LUq,6-q oo vzi.-Ol 1VA-._.', tot I 0.0 3.6 3-0 Ofo~~~ I iZ.0-Lo 1 0 .1

1 3.o 1

1 0 Note: If >I 5ml of titrant is used, sample must be diluted. Reviewed by: I Date reviewed I 1 ý5 - ý0(. ý

Page

"4

_Environmental Testing Solutions, Inc. Page 2o Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3,'L Analyst yl-f Date analyzed ,-z o Time initiated: Time completed: ratnt normality and multiplier determination: Titrant No-rm-aliy-ch-eck-e_ End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml x 50000)l/*X ml sample nnumber umber (E) (acceptable range = 0.018 - 0.022) r-D-.o0 0 numbr (E - A7C 1 Laboratory control standard: Reference standard True value Sample Hardness (MV) 0% RS =MV/TV x 100 number (TV) volume Begin End Total Multiplier (mag CaCO 3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml = 90 to 110%) 40 Ao50 LAI 102. Duplicate sam ple precision.: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOVL) ((S - D) /I(S+D)/2() x 100 number "l'j (ml) ml ml ml (acceptable range = +/- 10%) Duplicate (B) 3c D

O Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Mg CaCO3/L) (mg CaCO3 L) (ml) ml ml ml Sample hardness (B) Measured spike value (NIV) % R =MV /SV x 100 (mg CaCO3/L) MV= A - B (acceptable range (mg CaCO3/L) = 75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3IL) Blank (should be = 0 mg CaCO3/L) oW1,10z, ot A i-6A -) K 7 5b a3. A9-4, ao.L 4-'~t

z.

I _______o 06170,0L2_. -**3.0.. 3._ 00-. Ol-_____ -I X' L Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: D e ed-/ Datereviewed

Environmental Testing Solutions, Inc. Page 2-of L Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed 12-03 .-by Titrate samples to pH = 4.50 S.U. Titrant normali and multidlier determination: pH of - l Normality (N) of H2 SO4 pH Factor or Multiplier Deionized Titrant check Begin -- tl (5 ml Na 2CO 3 x 0.05)/E =(Nx 50000)/ 100 ml sample water reference standard ml ml ml 0.25/E = N x 500 4.5 S.U. number number (E) (acceptable ran`g-e-&.410.2). Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOs/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml 90 to 110%) ssq 100 100 3q0 q e,.q 12 Duplicate sam ple precision: Sample 0.0 to.5" to.S-Alkalinity %RPD= Sample Sample ID volume Begin End Total Multiplier (Mg CaCO 3/L) {(S - D) /[(S+D)/21} x 100 number (TVA (ml) ml ml ml (acceptable range = +/- 10%) (5~~ .v (11 0,,0-00 6$0,nT1.o I 6&N lQI I Iot3 3a.o -4&4-*--4-@ 10.2 S/ao £f A/ Duplicate -. i I D V-15-1 Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3IL) (rag CaCO3 L) (ml) ml ml ml 14b5L-, but io (P-s-19.8 iL3 1o.z 120 Sample alkalinity (B) Measured spike value (MV) % R MV / SV x 100 (mg CaCOJL) MV = A - B (acceptable range (mg CaCO,/L) = 75 to 125%) jYOl.0L. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (MIg CaCO3/L) WOc k C) I.M 10 2-f1 0C) 1'4 6 A~ 5141 IoO (v a* 0%4 o**- L-¢A- ,x to. () Aq.-3

.-36,e IO.

(0oo It 2-.08 3T.2 '13.6 V., v*

ol. o7.-,I

.3

uq &.9 bOq

-S557 DE LIS bio* 1_ 3R.- Nq. 1,?) 19 3L_ Reviewed by: Date reviewed: I I?.-O*-!Q(

Sequoyah Nuclear Plant Biomonitoring November 28 - December 5, 2006 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, Inc. Potassium NO Pimeph ales prom elas Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1, USEPA I C L 2 S USEPA Control Limits (+/- 2 Standard Deviations) 0.8 F 0.7 i- -Q 0.6 0.5 1.2 1.0 0.8 0.6 0.4 I I. I I I I~ __ ~ I I I I I I I 0.9 0.8 0.7 0.6 0.5 0.4 Test date 7-day ICs2 5 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC25) Warning Limits (mean IC2 5 +/- SA.10 or SA.75) Control Limits (mean IC 25 +/- SA25, S A.901 or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Pimep h ales pronmelas Potassium ChlorideChronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Test number Test date 7-day IC2, CT (g/L KCI) (g/L KCI) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 02-07-06 0.67 03-07-06 0.61 03-14-06 0.53 04-04-06 0.57 04-18-06 0.64 04-18-06 0.61 05-09-06 0.57 06-06-06 0.45 06-13-06 0.56 07-11-06 0.61 07-18-06 .0.61 08-01-06 0.64 08-08-06 0.67 08-15-06 0.49 08-22-06 0.65 09-19-06 0.61 [0-10-06 0.60 10-17-06 0.64 11-14-06 0.54 11-28-06 0,63 0.64 0.61 0.60 0.61 0.61 0.60 0.58 0.58 0.58 0.58 0.59 0.60 0.59 0.59 0.59 0.59 0.60 0.59 0.60 State and USEPA Laboratory Laboratory S Control Limits S,0 Warning Linits SA,2 5 Control Limits SA76 Wa2 CT-2S CT+2S CT - SAC CT + S,Io CT - Sns CT + S,,s CT - S, 0.04 0.56 0.72 0.08 0.57 0.72 0.13 0.51 0.78 0.24 0.40 0.07 0.47 0.74 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.06 0.48 0.72 0,07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.06 0.49 0.72 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.05 0.51 0.71 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.05 0.51 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.07 0.44 0.72 0,07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.07 0.45 0.71 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.06 0.46 0.71 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.06 0.47 0.70 0.07 0.51 0.65 0.12 0.46 0.7L 0.22 0.36 0.06 0.47 0.71 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.06 0.47 0.72 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.06 0.46 0.72 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.06 0.46 0.72 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.06 0.48 0.72 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.06 0.47 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.06 0.48 0.71 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 USEPA USEPA "ning Limits SA.90 Control Limits CV 75 CT + S,,, CT - SA.90 CT + S*_o 0,89 0.29 0.35 0.84 0.27 0.33 0.82 0.27 0.33 0.84 0.27 0.33 0.84 0.27 0.33 0.83 0.27 0.33 0.80 0.26 0.32 0.80 0.26 0.32 0.80 0.26 0.32 0.81 0.26 0.32 0.81 0.27 0.32 0.82 0.27 0.33 0.81 0.26. 0.32 0.82 0.27 0.33 0.82 0.27 0.33 0.82 0.27 0.33 0.82 0.27 0.33 0,82 0.27 0.33 0.82 0.27 0.33 0.93 0.06 0.88 0.11 0.86 0.10 0.88 0.09 0.88 0.08 0.87 0.08 0.84 0.12 0.84 6.11 0.85 0.11 0.85 0.10 0.86 0.10 0.86 0.10 0.85 0.11 0.86 0.11 0.86 0.11 0.86 0.10 0.87 0.10 0.86 0.10 0.86 0.10 Note: 7-d IC15 = 7-day 25% inhibition concentration. An estunation of the concentration of potassiusi chloride that would cause a 25% reduction in Pihnephales gowth for the test population. CT = Central tendency (mean IC25). S ý Standard deviation of the ICý, values, Laboratory Control and Warning Limits Laboratory control and warning lWfits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs, These ranges are miore stringent than tht control and warning limits reconmmended by USEPA for the test method and endpoint. S, 10 5 Standard deviation corresponding to tle 10t' percentile CV. (S, =0.12) Standard deviation corresponding to die 25" percentile CV. (S5 25 0.21) USEPA Control and Warning Limits Sý.5 = Standard deviation corresponding to the 75" percentile CV. ( Sx75 = 0.38) S = Standard deviation corresponding to dite 90" percentile CV. (So 5 0.45) CV = Coefficient of variation of the 'C25 values. USEPA. 2000. Understasding and Accounting for Method Vriability in Whole Effluent Toxicity Applications Utnder the National Pollutant Discharge ElOaiioation Psogpam. EPA-833-R-00-003. US Enviroouneatal Protection Agenscy, Cincinati. OH. Organism~s oblai-sdfrorn Aquatic.ticSyst ems. Inc. 1-00 11-28 06

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimephales promelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test number Test date Control Survival Control Mean Growth CT. CV CT MSD PMSD CT for Control Growth (%) (g/larvae) (mg/larvae) for Control Growth CV (%) (%) for PMSD (%) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 02-07-06 03-07-06 03-14-06 04-04-06 04-18-06 04-18-06 05 06 06-06-06 06-13-06 07-11-06 07-18-06 08-01-06 08-08-06 08-15-06 08-22-06 09-19-06 10-10-06 10-17-06 11-14-06 11-28-06 100 100 100 100 100 100 100 100 97.5 100 97.5 100 100 100 100 100 100 100 100 100 0.711 0.774 0.745 0.766 0.621 0.668 0.841 0.783 0.709 0.673 0.623 0.746 0.613 0.765 0.768 0.699 0.685 0.781 0.737 0.621 0.743 0.743 0.749 0.723 0.714 0.732 0.739 0.735 0.729 0.719 0.722 0.713 0.717 0.720 0.719 0.717 0.720 0.721 0.716 7.8 8.4 9.7 11.5 9.7 13.3 7.5 5.9 8.2 2.5 17.1 10.5 10.4 8.0 8.7 12.7 5.1 7.3 5.3 14.0 8.1 8.6 9.4 9.4 10.1 9.7 9.2 9.1 8.5 9.3 9.4 9.4 9.3 9.3 9.5 9.2 9.1 8.9 9.2 0.09 0.10 0.14 0.08 0.09 0.10 0.12 0.08 0.16 0.09 0.10 0.13 0.08 0.16 0.12 0.12 0.10 0.15 0.10 0.12 13.2 13.3 18.9 10.7 13.8 15.4 14.4 9.6 22.0 13.1 16.6 17.0 13.6 20.4 15.5 17.2 14.0 18.7 13.4 18.7 13.2 15.1 14.0 14.0 14.2 14.2 13.7 14.6 14.4 14.6 14.8 14.7 15.1 15.2 15.3 15.2 15.4 15.3 15.5 Note: CV - Coefficient of variation for control growth. On average, the CV for control growth is 9.2% in Environmental Testing Solutions, Inc. Pimephales chronic toxicity tests. Lower CV bound detennined by USEPA (1 0th percentile) 3.5%. Upper CV bound determined by USEPA (90th percentile) 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatmient that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15. 5% from the control. Lower PMSD bound determined by USEPA (10' percentile) = 9.4%. Upper PMSD bound determined by USEPA (90th percentile) = 35%. CT = Central Tendancy (mean Control Growth, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatic BioSystems, Inc. 11-28-06

Environmental Testing Solutions, Inc. Pimephales promelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 0 Q 0 0 Q 0 0 0 Q C 0 cr2 2* 1.25 1.00 0.75 0.50 0.25 30 20 10 r1I I Cri terI I I Ivi I I I I v USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I I i I I I I I I I I I I I I I I I I I I I Kentucky Acceptance Limit (< 30.0%) 0 Q 0 0 0 0 40 30 20 10 n I I I I I I I I I I. I I I .I I I I I I I I I I I I I USEPA Upper PMSD Bound (9 0th percentile < 35.0%) I I I i I I I I I I I I I I I I I I I L/ " '0 ,0 11 0 6*. ' ý 0 0 c b 0.* j b IIx

, x,(,

O* 0,(a¢ Cab va 0* j II Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Environmental Testing Solutions, Inc. Page I ofr5 EnirnenalTstn So.inIc.Pg t Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: J010 Test organism information: Test in ormation: Organism age: 14 ZS" -rL "Zt., "IS C c' e*,. Randomizing template: iou"Le. Date and times organisms t "o '600 Incubator number and were born between: shelf location: Organism source: g* {*Rxr,.* P*\\-1.1-06 Arternia lot number:

-r'd-0 Transfer bowl information:

pH = SU Temperature = 'C Total drying time: -04o0* I",

24.

Date/Time in: jtt-aoS-o '"-, lz Average transfer volume\\ Date / Time out: Q-Cb6-*o I I2S I Oven temperature: two U. Daily feeding and renewal information: Day Date Mornung Afternoon Test initiation, MHS Analyst feedin feeding time renewal, or batch used time termination time 0 Al~P_______P 2 o hGI_152-_o _6_ 3___ 0___ ql 1i0106 4 ]- 6. 1bt,, Iq~ I% -s \\-L(,- 01P6 [ 5

17.

o,-1 /SZ5 1 /q (-Z.o*-o(OA ) Control information: Acceptance ci itel-if Sumniary of test endpoints: % Mortality: 520% 7-day LCýo JO". L Average weight per initial larvae: O. 62 I NOEC _O Average weight per surviving larvae: Ž 0.25 mg/larvae LOEC ChV -5t q. (. IC25 I (-2T. 9

Environmental Testing Solutions, Inc. Page 2 of 5 Environmental Testing Solutions, Inc. Pace2 ot5 Species: Pinephales promelas PpKCICR Test Number: 10O Survival and Growth Data Day Control 300 mg KCI/L 450 mg KCI/L A B C D E F G H I J K L 0 I.) Io /O ]o 10 It 10 /0 to /0 /) 1t 1 3o lD /0 /0 t0 /0 10 /0 /0 /0 /0 /0 10 10 /0 t0 I O /0 (/0 3 tO /0 to /0 /0 /0 to /10 /0 /0 D 0 4 I0 /0 10 tO5 tO /0 (0 10 '0 I IQ (0 / iO /0 (0 /0 /O /0 /0 /03 /0 /0 /0 /0 6 O /o / /I to /0 /0 1o /0 /o /o /0 7 1 -A. /0 /0 '0 /0 /0 /C /0 '0 /D /o /C /O A = Pan weight (mg) Tray color code:: UI Ne Maq 1 Analyst: i?* IqLS 1.Z3 .o t I.5(O I.45 6q." o13.15 41ID 15.0Q jq.4c rq.OI k. B = Pan + Larvae weight Analyst: LABqa.5a.~l.qa/{ tl !8 ~~~ La to ac o4 Larvae weight (rag) = A - B ... g o,"z-7,4 5 .' I,,.Z% 6.1-5.9 14-61 t,.'S7 Sa (65 O,'Z. Weight per initial numbnerA x of larvae (nag) "A, \\ \\ .\\ A = C/hfInitial number of larvae %V o" o" t o" o o b" D. o" O' Average Percent weight per reduction initial from control O, { 2-number of (% larvae (mn) A::. N **?: ::,' Comment codes: c = clear, d = dead, fg= fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Page 3 of 5 Species: Pimephales prom elas PpKCICR Test Number: 10 Survival and Growth Data Day 600 mg KCI/L 750 mg KCI/L 900 mg KCL/L N1M N 0 P Q R S T U V W X .0 0_ IL) /0 I O // /0 A- /) I)0 /0 16 1 14 .4q - 1 IA k4 S a /0 c3C to (,,q4 2.q'4 /0 ID '2. 2 10, g 4 (, '4 34 ',4 2-A 2.. 3, Lk LI 4 (1 L4 6 Lk I~ z ' I Z 6 '1 'S 4 LA q 7 3 % I 0 t 7 iq C A =-Pan weighA (11g) rrav color code:: 1 1.3.rlq NL3.`r 133.3 1*.M Ijq.11 13.,65 f'-.. 14.30 i0 rg.r Anialyst: {04i4* B = Pan + Larvae wveight Analyst: UL43. gB O.5lltl qqI*l' tr 5'},* 1£ 5o Larvae weight (nmg) = A - B t 4. $ -~" i g L q * % 2 q

L "£ < °.,So l

1, o.. Z, O 1. 2 1 k Weight per initial number4)A of larvae (g) = C / Initial num ber of larvae '3" b "* O " 0 00" 0* 10' 10 V-~ c 0 Average Percent weight per reduction initial from control 0... ,.2 number of (%) larvae (rig) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm= unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimeph ales prom elas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR H 146 (#106 at 351 Depot St.) Test dates: November 28 - December 05, 2006 Reveiwed by: Concentralion (mg/L Replicate Initial numberof Final number of A=Panwaght(mg) B-Pan+L-aa La-ae eight (mg) Weight/Surviving Me.n. -igho C Coefcientof ofvaration Weight /IInitial number M... urvia n Monweight Initial Coefficientof variatioon Percenl reduction from KCI) I.-ae larvae weight (mag) = A - B number of lrv-e (r.g) Suriving noumber of (M.n

p',

of Iory.- (mg) (% .nuber of larvon 5%) control (%) A 10 10 14.55 20.37 5.82 0.582 0.582 Control B 10 10 13.96 20.18 6.22 0.622 0.621 14.0 0.622 100.0 0.621 14.0 Not applicable C 10 10 13.64 21.05 7.41 0.741 0.741 D 10 10 14.56 19.94 5.38 0,538 0.538 E to 10 14.85 21.06 6.21 0.621 0.621 300 F 10 10 14.78 21.51 6,73 0.673 0.580 14.9 0.673 100.0 0.588 14.9 5.3 G 10 10 13.95 19.85 5.90 0.590 0.590 H 10 10 14.62 19.29 4.67 0.467 0.467 1 10 10 15.05 21.32 6.27 0.627 0.627 450 J 10 t0 14.49 21.03 6.54 0.654 0.609 7.0 0.654 100.0 0.609 7.0 1.9 K 10 10 14.01 19.55 5.54 0.554 0.554 L 10 10 14.44 20.46 6.02 0.602 0.602 M 10 7 13.74 18.29 4.55 0.650 0.455 600 N 10 9 ,14.37 18.4 4.47 0.497 0.654 27.0 0.447 80.0 0.520 26.0 16.3 0 10 8 13.33 20.55 7.22 0.903 0.722 P 10 8 15.06 19.61 4.55 0.569 0.455 10 5

14. lI 17.04 2.93 0.586 0.293 750 R

10 4 13.85 16.17 2.32 0.580 0.593 6.4 0.232 40.0 0.238 22.2 61.7 S O 10 4 14.28 16.87 2.59 0.648 0.259 T 10 3 14.30 15.98 1.68 0.560 0.168 U 10 0 0.00 0.00 0.00 0.000 0.000 900 V 10 1 12.90 13.50 0.68 0.680 0.650 6.5 0.060 7.5 0.048 124.9 92.3 W t0 0 0.00 0.00 0.00 0.000 0.000 X t0 2 13.77 15.01 1.24 0.620 0.124 Dunnett's MSD value: PMSD: 0.1163 18.7 MSD = PMSD = Miniotwn Significant Difference Percent Minisum Significant Difference PMSD is a measure of test precision. The PMSD is the osinuhnnus percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environoental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.5% front the control (deternined through reference toxicant testing). Lower PMSD bound detenisined by USEPA (10th percentile) = 9.4%. Upper PMSD bound detenmined by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted fronn 19 laboratories for Pitnephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinsination Program. EPA-833-R-00-003. US Environntental Protection Agency, Cincinnati, OH. Organisms obtainedfromn Aquatic BioSystems. Inc. 11-28-06

Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 DaySurvival Start Date: 11/28/2006 Test ID: PpKCICR Sample ID: REF-Ref Toxicait Fnd Date: 12/5/2006 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-PoLassium chloride Sample Date: Protocol: FWCHR-13PA-821-R-02-013 Test Species: PP-Pirnephales promelas Comments: Conc-mg/L 1 2 3 4 D-Conteol 1.0000 1.0000 1,0000 10000 300 10000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 0000 600 0.7000 0.9000 0.8000 0.8000 750 0.5000 0.4000 0.4000 0.3000 900 0.0000 0.1000 0.0000 0.2000 Transform: Arrsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 300 1.1000 1.0000 1.4120 1.4120 1.4120 0,000 4 10.00 10.ý00 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 1000 0 40 '600 0.0000 0.8000 1.1136 0.9912 1.2490 9,478 4 10.00 30.00 8 40 '750 0.4000 0.4000 0.6836 0.5796 0.7054 12.289 4 10.00 10.00 24 40 1900 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4 1000 10.00 37 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Walb's Test indicates non-normal distribution (p <= 0.01) 0.795636058 0.684 0.52113979 1.618140669 Equality of variance cannot be confmned llyputhlsis Test (1-tail, 0.05) NOEC LOEC ChV TU Steels Many-One Rank Test 450 600 519.6152423 Treabnents vs D-Conttol Maximum Likelihood-Probit Parameter Value SE 95% FiduCial Limits Control Chi-Sq Critical P-value Ma Sigma lter Slope 13.16756983 1.804761012 9.630238175 16.70490148 0 0.603478868 7.814727783 0.9 2,849547769 0.075944158 3 Intercept -32.5216192 5.153545422 -42.6225685 -22.42067 TSCR 1.0o Point Probits mg/L 95% Fiducial Limits 0.9 ECO 2.674 470.&420465 400.1998345 519.0476467 EC05 3,355 530.4332572 469.5403967 571.9700907 0,8 ECI0 3.718 565.2250698 510.7644752 602.9745341 0.7 ECI5 3.964 589.9792149 540.2163463 625.2854703 PC20 4.158 610.4239368 564.4820016 644.0018976 0v EC25 4.326 628.5273978 585.815420 660.900742 00.5 EC40 4.747 676,5619012 640,8299499 708A1026"87 EC50 5.000 707.2089864 673.905148 740.8218287 0 EC60 5.253 739.2443377 706.2829966 777.6913987 0.3 EC75 5.674 795.7402488 75&.3724828 848.8783814 0.2 EC80 5.842 819.3396792 778.711648 880.4631134 EC85 6.036 947.7325071 802,4164168 919.5582422 0.1 EC90 6.282 884.8590825 832:4719031 972.1556955 0.0 EC95 6.645 942.8981527 877.9592221 1057071685 10 100 1000 10000 RC99 7.326 1062234314 967.8556655 1239739429 Dose mg/L Dose-Rmponse Plot 0 Organi/ss obtained from Aquatic BioSystem3, Inc. 11-28-06

Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/28/2006 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 12/5/2006 Lab ID: ETS-Eaiva. Testing Sol Sample Type: KCL-Potassitun chloride Sample Date. Protocol: FWCIAR-EPA.821-R-02-013 Test Species: PP-Pimephales promelas Cossmmnenits Cone-mg/L 1 2 3 4 D-Control 05820 0.6220 0,7410 0,5390 300 0.6210 0.6730 0.5900 0,4670 450 0.6270 0,6540 0.5540 0,6020 600 0,4550 0.4470 0.7220 04550 750 0.2930 0.2320 0.2590 0.1680 900 0.0000 00680 0.0000 0.1240 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L MINn N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0,6200 1.0000 06208 0.5380 0.7410 14.047 4 0.6208 L.000N 300 0.5878 0.9468 0,5878 0.4670 0.6730 14,884 4 0.619 2.100 0.1163 0.5985 0,9642 450 0.6093 0.9815

0.6093 0.5540 0.6540 6.978 4

0.236 2.180 0.1163 05995 0.9642 600 0.5198 0.8373 0.5198 04470 0.7220 25.952 4 0.5190 0.8373 750 0.2380 0,3834 0.2380 0.1680 0.2930 22.236 4 0.2300 0.3834 900 0.0480 0,0773 0.0480 0.0000 0.1240 124.907 4 0.0400 0.0773 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wi~c's Test indicates norsmal distribution (p > 0.01) 0.989200532

0. 805

-0,0220246 -0.11689876 Bartlett's Test indicates equalvariances (p = 0.48) 1.461641073 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDU MSDp MSB MSE F-Prob d[ Diurnet's Test 450 >450 0.11625787 0.187286138 0.001122333 0.005680028 0.824389994 2,9 Treatnments vs D-Contaol Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05 466.74 17537 000 699.35 -0.3523 lClo 525.86 120.25 0.00 683.50 -1.3121 IC15 504.98 74.33 115.33 668.60 -1.9295 1.0. 1C20 612.32 44.55 432.71 67224 -0.8215 0.9 - 1C25 628.85 34.38 485.90 602.25 -0.8810 1C40 678.42 19.72 60793 716.26 -0.4679 0.8 - ICS0 711.47 15.17 657.93 746.32 -0.2958 0.7 00.6 LC 0.5 0.3 0.2 0.1 0.0 0 200 400 S00 300 1000 Dose mg/L Dose-Response Plot 1-tail, 0.05 level of significance -6 a C Organisms obtained from Aquatic BioSy.stems, lnc. 11-2M-6

Environmental Testing Solutions, Inc. Page 4 of 5 Environmental Testing Solutions, Inc. Page 4 of 5 Species: Pinephales promelas PpKCICR Test Number: 10(o Daily Chemistry: Day 0 1 2 Analyst I j' K-k- Concentration Parameter CONTROL pH (S.U.) Ia' Q'5 '4. .5-DO(rgiL) Conductivity e 3e. 5___ (p mhos/cm) 3_________ Alkalinity ~ (mg CaCO.I/L) H-ardness (mg CaCO 3/L)

-z Temperature z;

.Z - 2L 1 _ _ _ _ (°C) 2--_" pH (S.U.) ",95. 26, /4 -(a DO (mg/L) t49 .o, "4-300 mg KCI/L Conductivity (1imhos/cm)______ Tem perature

2.

2

,A

-D -to .v 4 (0c) .1 pH (S.U.) '.5a '4,163 q.OL -. 5 DO (mg/.) 9.* 450 mg KCI/L Conductivity "14o II.:D Temperature (°C) 4.___ pH (SU.) .94 .5 DO (mg/L) 2.6 _ _3.O (0.(, 600mg KCI/L Conductivity 1q30 I, O (p rnhos/cm) Temperature. 1%1 Z%2. 0 -z* A0 0 (oc) 2 pH (S.U.) ,3j0 9, '.T . 3-- DO (mg/L) '.4 '+- (30 _ 1 750 mg KCI/L Conductivity (Prmhos/cm) Temperature zq. VA 1q. (0 214I Lq.q ( 0C ) L PH (S.U.) 9"64 Iq A 6-7 "ip( ".DO DO (mg/L) -,.( .0.'-/ 900 mg KCIJL Conductivity j;K (PiMhos/CM) H3___ ____0 ~ l Temperature 114. L4 STOCK Conductivity)q zcO z%.,. STOCK Ci-*,p' ,"hos/cn) .,,,...o 4..........,, Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final

Environmental Testing Solutions, Inc. Rice 5 of 5 Environmental Testing Solutions, Inc. Page 5 ots Species: Pimephales promelas PpKCICR Test Number: INP Day 3 4 5 6 Analyst VL -. Va'- Concentration Parameter-- CONTROL pH (S.U.) 0 ,I5 j "¶, ".O 20 .2 4.,a! 5 '4.( DO (mg/L) (2.- q 2.-_ 4-6 Conductivity .4 (pimhos/cm)___________ Alkalinity (m.g CaCO 3/L) Hardness j D\\ (mgCaCO3 /L) Temperature L A -R 'A (°C) -q. -q' -q "-\\- 5" -q. pH(S.U-) 5 4 qI %.f 'ý.iZ jqj 93 72A 3 1,-?- DO (mg/L) qAff q.' 2.. 300 mg KCI/L Conductivity (pmhos/cm) ~ S2 24 Temperature Z_ (°C) pH (S.U.) .5 .,q -3 2_ 3o D O ( m g / L ) 2 - -Q 3" .} .Z

q" 450 mg KCUL Conductivity 54, (iamhos/cm)

Roo___ _____7 ~ Temperature 2q.S -(-q. I.A ."-"z u (C) pH (S.U.) I.fl q q 9.5-w( "2i 600 mg KCIL DO (mg/L) 4,1 ,9q iiL JL Conductivity Temperature 2q.k -N. VA*1 (°C) 2_._o I_. PH (s.U.) q.g 1 1,02-,* '1q0o "q4 qo ?.A3 '., 9.q 1-,< DO (Sg/L) b oo q LI0 Q

q. 3 750 mg KCUL Conductivity,.o
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(p m ho s/c m ) 1q _0......... I________. Temperature -l,1 2q2.S 2 L. .0 "Z,4.

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Final, Initial Final Initial Final I Initial Final Initial Final I

I I.

Environmental lesting solutions, Inc. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 1.10 1.08 U EI I I (-I I I I StaI DevIa ions) USEPA Control Limits (+ 2 Standard Deviations) 1.06 F-Qn 1.04 2.5 2.0 1.5 1.0 0.5 1.4 1.3 l I I I I I I ] I I USEPA Warning and Control Limits (75th and 90th Percentile CVs) I I I I 1I I I I I I I I I I I I I II I I I I I I I I I I I I I I I 1.2 1.1 1.0 I I I I I I I I I I I I I I I I I I Laboratory Warning and Control Limits (10th and 25'h Percentile CVs) I I I I I I I I I I I I I 0.9 U.* Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC 25) Warning Limits (mean IC25 +/- SA-or SA,7) Control Limits (mean IC25 +/- SA.25, SA.90, or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC2s CT S Control Linits SA.to Warning Limits SAs Control Limits SA.ls Wanting Linits Control Linits CV (g/LNaCI) (g/LNaCI) CT-2S CT4+2S CT - S,,, CT + SA1 o CT - S&2s CT + S, 25 CT - S1s7 CT + S17s CT - S. 9, CT + S,,, 1 06-07-05 1.05 2 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 3 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 4 08-09-05 1.08 1.06 0.01 1.04 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 5 09-13-05 1.04 1.06 0.01 1.03 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 6 10-04-05 1.09 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 7 11-01-05 1.08 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0,88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.02 8 12-06-05 1.04 1.06 0.02 1.03 1.10 0.08 0.98 1.15 0.18 0,88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 9 01-10-06 1.08 1,06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 10 02-07-06 1.07 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.02 11 02-07-06 1.06 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0,88 1.24 0.48 0.58. 1.54 0.66 0.40 1.72 0.01 12 03-07-06 1.05 1.06 0.02 1.03 1.09 0.08 0.98 1.15 0.18 0,88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 13 04-04-06 1.06 1.06 0.01 1.03 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 14 05-02-06 1.08 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 15 06-06-06 1,06 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0,40 1.72 0.01 16 07-11-06 1.09 1.06 0.02 1.03 1.09 0.09 0.98 1.15 0.18 0,88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 17 08-08-06 1.07 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0,88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0,01 18 09-12-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0,88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 19 10-03-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0,01 20 11-07-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0,88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 Note: 7-d IC25 5 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25). S = Standsrd deviation of the IC 2s values. Laboratory Control and Wanting Limits Laboratory control and warning linits were established using the standard deviation of the IC 25 values corresponding to the 10th and 25th percentile CVs. These ranges are nsore stringent than the control and warning limits reconrnsended by USEPA for the test miethod and endpoint. S&10 = Standard deviation corresponding to the 106h percentile CV. (SA0 = 0.08) SA,5S = Standard deviation corresponding to the 25a percenstile CV. (S.2s = 0.17) USEPA Control and Wanting Linuts SA_5 = Standard deviation corresponding to the 75t" percentile CV. (SAS = 0.45) S,90 = Standard deviation corresponding to the 90'h percentile CV. (SAs0 = 0.62) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinsination Progratn. EPA-833-R-00-003. US Environmental Protection Agency, Cincimnati, OH. 10-03-06

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test Test date number Control Survival Control Mean Reproduction CT CV CT MSD PMSD CT (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 06-07-05 07-12-05 07-12-05 08-09-05 09-13-05 10-04-05 11-01-05 12-06-05 01-10-06 02-07-06 02-07-06 03-07-06 04-04-06 05-02-06 06-06-06 07-11-06 08-08-06 09-12-06 10-03-06 11-07-06 100 100 100 100 100 100 100 100 100 100 100 100 100 t00 100 100 100 100 100 100 30.4 30.4 31.1 28.3 27,9 27.0 28.4 32.6 29.2 30.7 29.9 28.8 27.0 28.6 30.3 29.0 28.6 30.9 32.3 31.0 for Control Mean Reproduction (offspring/female) 30.4 30.6 30.1 29.6 29.2 29.1 29.5 29.5 29.6 29.6 29.6 29.4 29.3 29.4 29.4 29.3 29.4 29.5 29,6 for Control (%) Reproduction CV (%) (%) for PMSD (%) 5.0 7.5 7.2 7.3 7.3 5.8 10.3 6.3 4.8 6.0 6.8 5.9 5.2 8.6 5.2 5.4 8.9 5.4 4.6 6.3 6.2 6.5 6.7 6.8 6.7 7.2 7.1 6.8 6.7 6.7 6.7 6.5 6.7 6.6 6.5 6.7 6.6 6.5 6.5 2.6 2.7 3.2 2.9 3.9 3.0 3.8 2,3 2.3 2.4 12 2.6 2.1 2.7 3.0 25 3.7 3.3 3.1 2.4 8.5 8.8 10.2 10.3 13.9 11.1 13.2 7.1 7.8 7.9 7.5 8.9 7.6 9.3 9.8 8.6 12.8 10.6 9.5 7.8 8.7 9,2 9.5 10.3 10.5 10.9 10.4 10.1 9.9 9.7 9.6 9.5 9.4 9.5 9.4 9.6 9.7 9.7 9.6 Note: CV= Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.5% in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity tests. Lower CV bound determined by USEPA (10 O'percentile) = 8.9%. Upper CV bound detennined by USEPA (90" percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA (10 th percentile) = 11%. Upper PMSD bound determined by USEPA (90 't percentile) = 37%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) The lower and upperbounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reterence toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent ToxicityApplications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, 011. 21-07-06

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 60 Z-~* E. 4. 40 35 30 25 20 15 40 30 20 10 40 30 20 10 A Minimum Acceptance Criteria (> 15.0 offspring per surviving female) I I F F F I I I t I rI F I I F I F I I I I I I I I I I North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%) I I I I I F I I I I I I I I I I I I I USEPA Upper PMSD Bound (9 0 " percentile < 37.0%) L/ Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Environmental Testing Solutions, Inc. Page I of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCRA#: 5 Dilution preparation information: Comments: NaCI CHM number: (_.A M I -L.{3 Stock preparation: 100 g NaCI/l (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (m/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism information: Test information.: Organism age: < 24-hours old Randomizing template: co-A e),. Date and times organisms i\\s-A-Tro "70c7-. Incubator number and were born between: shelf location:. Organism source: Io.3\\-o6 A UK-o 1 rS S. YCT batch: Transfer bowl information: pH =-1.8. SU Temperature = CC Selenastrum batch: . -zab, Daily renewal information: Day Date Test initiation, MHS water Analyst renewal, or batch used termination time 0 II-om-06t t\\2 1-2"1-o 6 I I -t-CA_ 15-2.1 - l 0 (0 2 11 Oq-OD 117,0 10"v',Io C.. 3 / 1-t0 - 0 6.b 1 ( t O -Z I o, L. 4__ I01I-SlH-11,06 I oq. io ' 511 N - Oofo 6 ",4 vto nJk.c Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: o

t 20%

7-day LCSO 1400 % Adults having 3 Broods: m_ 80% NOEC 1000C % Mortality:

7,

< 20% LOEC o Mean Offspring/Female: ,31. 0 > 15.0 offspring/female ChV L. % CV: < 40.0 % 1C25 _____._0

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: SS CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 Young produced Q Q* t 0 _c), 0 [ 7 7 Adult mortality' k._ Q.-

L k._.

orat _C-1C _I C 2g Yougproduced o C 3 Young produced Adult mortality Yugproduced 4 Adult mortality 6 Young produced 10 C) 0~o C~' t[~[~ Io 7 J Young produced 16, I VS k" s 11 1*A I [' IS Total young produced v-b- s-2.z

2. ýLa 2 C1 Final Adult Mortality I...-

\\__ k____ k..._- t..., Xfor 3 d Broods )<- t Note: Adult mortality (L live, D = dead) Concentration: % Mortality: f 07J Mean OffspringFemale: 600 mg NaClfL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 1 9 10 I Young produced C ) 0 0C) 0 0 -) Adult mortality `,_... (- L-- I Lk-- I _--- I ..._ I t.-- Adult mortality I L X U \\ \\. L.. 2 Young produced 1 L Adult mortality \\-I 3-- I k-- %-- I__ 8_fci 5 Young produced o t \\ Adult mortality 6 Young produced____ Adult mortalityiIc I l I ] l V 7 j Young produced )6 .18 I1"A 5 I"1 iS Is q j R 4 Total young produced

32.

3$ -"o 5 Z 3,C Final Adult M ortality

i...

k.._..... Note: Adult mortality (L = live, D = dead) Concentration: % Mortality:

011, Mean Offspring/Female:

.31.1 % Reduction from Control: -2.3.

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia 9 800 m-NaCI/L CdNaCLCR 4: S5$ Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 S Young produced c 5 0 © © ( °dI r 0 _9 I If + J "7 Adult mortality j*- I YIVoung produced (5 b_ c_ c) 0_ 1 c) 01 0-Adult mortality 3 1on produced 101 0 1 C)10cI - [ c1o 0 c Adult mortality I... I__'- I_ -I__I Young produced IS 5 Young produced L2-Q Adult mortality l Young produced

c.

i1 Adult mortality \\,~ JU-[L 7 Young produced _9 cl " i [ioJ cbj c, L oI j [1 0 Total young produced -32 Zc 9 '*7 ,*. 0 .1 1 0 I' Final Adult M ortality k-.. L.. Note: Adult mortality (L live, D = dead) Concentration: % Mortality: Mean Offspring/Female: 3 1. 0 % Reduction from Control: 0 7. 1000 mg NaCI/L Survival and Reproduction Data Replicate number

Day, 1 1 2 3

1 4 5 6__L 7 1 9 10 I 1 Young produced to IICD_ I_ _0 C_ G _Z Adult mortality k-. ( 1 I L-Young produced C) .C) i 0 CL Adult mortality k&\\j j~ [ ~ U L I-k-L Young produced 1 C C o I CT Adult mortality I.. Young produced ( Adult mortality 5 Young produced V FjqJQ 10 l '0

z. 1 i 0

6 Young produced e C ý G I f510 Adult mortality U k[ C 7 Young produced LA 16 1 S IS i IS 3: It -7 Total young produced j_4q 7_-1 2.L 2'.1 M 31 z Z Final Adult Mortality U. U T.. \\. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: :7, Mean Offspring/Female: 30, I% Reduction from Control: 1 -s. Io

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR#: ý55 1200 m2 NaC/iL Survival and Reproduction Data Replicate number Day 1 1 2 3 4 5 6 7 1 8 9 10 1 Young produced 0 0 0 Q © 0 Q Ad1 mortlit k_._ Adult mortality

k.

L I 0 - 2 Young produced c_ Adult mortality Young produced

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0 Q C) L l Adult mortality J\\j__ I I__ -- ~- 4 Young produced 7_ 212-TO I -a-I AI' Adult mortality v-J.._ S Young produced 1Q f-3 j 2-uJo Adult mortality 'Young produced0 ___7 Youn prdue 0 00 C)O Total young produced [ ) Z O~'z I~ LA 0 e Final Adult Mortality -C: (T vL L \\.- k-Note: Adult mortality (L live, D dead) Concentration: % Mortality: o7. Mean Offspring/Female: 9.1 % Reduction from Control: -1 o,., 1400 m. NaCIJL Survival and Reproduction Data Replicate number Day 2 3 4 5 6 7 8 9 10 1 Young produced 0 10 0 .16 0 0 0 0 Adult mortality 1, IL1 0 L*L L

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O q 5 Z Final Adult Nfortality k_. L Note: Adult mortality (L= live, D dead) Concentration: % Mortality: 7j"* Mean Offspring/Female: -z. % Reduction from Control: i'A7. ci

Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control Replicate number Total D 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 5 5 5 4 5 5 4 4 4 47 5 10 0 0 0 0 0 0 0 0 0 10 6 0 13 10 11 12 12 9 13 10 10 100 7 16 15 15 15 113 17 18 15 14 15 153 Total 32 33 30 31 129 34 32 32 28 29 310 600 mg NaCI/L Re licate number Day 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 .5 5 5 5 4 6 4 4 47 5 0 11 0 11 13 0 0 0 0 0 35 6 12 0 10 0 0 13 11 11 10 12 79 7 16 18 14 15 17 15 15 14 18 14 156 Total 32 34 29 31 35 33 30 31 32 30 317 800 mg NaCI/L Re licate number Day 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 4 5 4 4 4 5 5 46 S 0 10 11 12 0 12 0 0 11 0 56 6 13 0 0 0 10 0 9 10 0 10 52 7 14 14 17 16 15 15 19 16 17 13 156 Total 32 29 33 32 30 31 32 30 33 28 310 1000 mg NaCI/L Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 4 4 5 5 4 4 5 4 45 5 14 10 0 10 11 10 12 12 10 10 99 6 0 0 9 0 0 0 0 " 0 0 0 9 7 14 13 16 15 15 18 15 13 17 14 150 Total 34 27 29 29 31 33 31 29 32 28 303 1200 mg NaCI/L Day Replicate number Total 1 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 2 2 0 2 4 1 1 3 3 21 5 10 0 2 8 2 12 0 5 5 3 47 6 0 6 0 0 0 0 2 0 0 0 8 7 0 0 6 0 8 0 1 0 00 15 Total 13 8 10 8 12 16 4 6 8 6 91 1400 mg NaCI/L Re licate number Day 1 2 3 4 5 6 7 8 9 10 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 2 0 0 0 1 0 0 2 2 1 8 5 5 0 0 0 0 4 3 0 0 0 12 6 0 3 0 0 0 0 0 0 2 0 5 7 0 0 0 0 0 0 0 .0 0 0 0 Total 7 3 0 0 1 4 3 2 4 1 25

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #84 (H55 at 351 Depot St.) Test dates: November 07-14, 2006 Reveiwed byýe-Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 1(%) (offspring/female) variotion (%) control (%) Control 32 33 30 31 29 34 32 32 28 29 100 31.0 6.3 Not applicable 600 32 34 29 31 35 33 30 31 32 30 100 31.7 6.0 -2.3 800 32 29 33 32 30 31 32 30 33 28 100 31.0 5.5 0.0 1000 34 27 29 29 31 33 31 29 32 28 100 30.3 7.5 2.3 1200 13 8 10 8 12 16 4 6 8 6 100 9.1 40.3 70.6 1400 7 3 0 0 1 4 3 2 4 1 80 2.5. 86.9 91.9 Dunnett's MSD value: PMSD: 2.417 7.8 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA (10h percentile) 11%. Upper PMSD bound determined by USEPA (90'h percentile) = 37%. The lower and ttpper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphnia Survival and Reproduction Test.Reproduction Start Date: 11/7/2006 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 11/14/2006 Lab ID: ETS-En'ar. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR -EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dbia Comments: Conc-,ng/L 1 2 3 4 5 6 7 8 9 10 D-Control 32.000 33.000 30.000 31.000 29.000 34.000 32.000 32.000 28.000 29.000 600 32.000 34.000 29.000 31.000 35.000 33.000 30.000 31 000 32.000 30.000 800 32,000 29.000 33.000 32.000 30.000 31.000 32.000 30.000 33.000 28.000 1000 34.000 27,000 29.000 29.000 31.000 33.000. 31.000 29.000 32.000 28.000 1200 13.000 8.000 10.000 9.000 12.000 16.000 4.000 6.000 8.000 6.000 1400 7000 3.000 0.000 0.000 1.000 4.000 3,000 2.000 4.000 1.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min May CV% N t-Stat Critical MSD M.can N-Mean D-Control 31.000 1.0000 31.000 28.000 34.000 6.270 10 31.350 1.0000 600 31.700 1.0226 31.700 29.000 35.000 5.958 10 -0.662 2.287 2.417 31.350 1.0000 800 31.000 1.0000 31.000 28.000 33.000 5.483 10 0.000 2287 2.417 31 000 0.9888 1000 30.300 0.9774 30.300 27,000 34.000 7.469 10 0.662 2.287 2.417 30.300 0.9665

1200 9.100 0.2935 9.100 4.000 16.000 40.276 10 20.721 2.287 2.417 9.100 0.2903 1400 2.500 0.0806 2.500 0.000 7.000 86.923 10 26.966 2.287 2.417 2.500 0.0797 Auxiliary Tests Statistic Critical Skew Kurt KolmogorovD Test indicates nsormal distribution (p > 0.01) 0.81748134 1.035 0.44619717 0.3712335 Barllett's Test indicates equal variances (p = 0.18) 7.55277777 15.0862722 Hypothesis Test (1-tail, 0.05)

NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prnb df Dunnett's Test 1000 1200 109544512 2.41677734 0,07796056 1738.96 5.58518519 1.6E-38 5, 54 Treatnents vs D-Control Linear Interpolation (200 Resarnples) Point mg/L SD 95% CL Skew IC05 1004.88208 51.0038904 825.635417 1014.14902 -2.5889 ICIO 1019.66981 5.96832498 1007.34438 1028.34195 -0.3117 IC15 1034.45755 5.61835965 1023.38686 1043.09093 -0.2720 1.0. IC20 1049.24528 5.40692453 1038.23334 1057.91483 -0.2188 0.9 IC25 1064.03302 5.35046783 1052.85837 1072.74883 -0.1422 IC40 1108.39623 6.09494531 1095.59506 1118.42537 0.2053 00 IC50 1137.9717 7.17272292 1123.85682 1151.46069 0.4115 0.7-0. 5 - 0.5 0.4 0.3 0.2 0.1 0.0 -0.1 0 500 1000 1500 Dose mg/L Dose-Response Plot 40 35 20 o 25 n 20 0 ~'15 10 1-tail, 0.05 level of significance 11-07-06

Environmental Testing Solutions, Inc. Page 5 o f 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: __.S Daily Chemistry: Day.. 0 12 Concentration Parameter _pH (S.U.) 9.8c2 '?A09.9 '.O

r, DO (magL)

93.

} CONTROL Conductivity (pmhos/cm) 2-12_ Alkalinity 5"k (mg CaCO3/L) Hardness (mg CaCO3 L) Temperature (0c ) 0 _ _ _,-1,_ pH (S.U.)

q.

cjq. q DO (m*.L, 600 mg NaCI/L Conductivity (pmhos/cm) Temperature IS. 2-I 2L1N U pH (S.U.) .o " .4. z DO (mg/L) (a

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800 mg NaCL'L Conductivity (Prhoslcm) I060 A10 ql Temperature (oc)_-____ 2.s. L*J 2*, 2 .qq 1 .-9 zq PH (S.U.) ?,0 9.2. DO (mg/L) , 9.q 1000 mg NaCI/L Conductivity (ýImhos/cm) 5bo 9 3too Temperature z, (0c) ,\\_ pH (S.U.) 9,-"./

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.'ý'36 ~ s 92. DO (rag/L) 11 1.) R. ~ 9 c (ýtmhos/cm) ?o,* 0 (0c) I DH (S.U.) II 0 '.I I Q.6? 1 ).qzA 2.9 I 931 4-1400 mg NaCIfL DO (mg/L) Conductivity (Pimhos/cm) Temperature (oC) 30ta Final " niia Fa0 -z q.G o -gO2 r "Z. Iq . (0 -z~q, t o Inita Final initial "Final

Initiaj

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Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: 5S Day _ 3 4 5 6 Analyst )LA d w I S Conentration Parameter tý'511 I'S M, MRIAMM4,4 MORM "M r, IMI'l-'11-11MR I i I PH(S.U. 4 7 4 ~e DO (mg/L) 0-.0. CONTROL Conductivity ( imhos/cm) 512. Alkalinity (mg CaCO3/L) Hardness (mg CaCO 3/L) Temperature (0c) -Lq 9 pHr (s.u.) q*o

Bo DO (mg/L) 600 mg NaCI/L Conductivity

( rmhos/cm) /5/5 Temperature (?C) .7 -N --I pH (S.U.) -0. 6-0q 9.& 6.01 C-3

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80 gNCL DO (mg/)

14.

'9& 96 0-i 8000 mg NaCL/L Conductivity (4rnhos/cm) 190 1 e,, Temperature -L. O (-C)N.- 1s-I0 pH (S.U.) ). ,.83 . 5 .O2- ".i D O (m g/L) ,-4 A,3 1000mg NCIIL Conductivity (pmhos/cm) P4O0 9ý)oD Temperature (OC)14. I, zg q z48 H(S.U.) W q6 DO_(ms u) all4 <)-:7 1200 mg NaCI/L Conductivity (rmhos/cm) o0 Temperature L (OC) pHo(S.U.) 9-3 9_, e.0-1400mg N~l/L Conductivity (pmhos/cm) pq Temperature F n 2Fnl na F Initia Fi (OC) STOCK Conductivity ~- (jimnhos/cm) Initial Final initial Final Initial Final Inta inal I I I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) 0MB No. 2040-0004 TN0026450 I 103G F-FINAL PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND MONITORING PERIOD I EFFLUENT YAiMO DAY DA From o6 O1 ToY mLY 0 1 DA213. NO DISCHARGE E *] NOTE: Read instructions before completing this form. ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.2 8.8 12 0 24/ 31 GRAB MEASUREMENT 00400 1 0 0 PERMIT

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persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 01 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, indluding the possibility of fine and imprisonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I _________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) The Turbine Building Sump (TBS) was discharged directly to the Yard Pond (YP) from 12/08/2006 @ 0024 to 12/13/2006 @ 2158 and from 12/20/2006 @ 1205 to 12/24/2006 @ 1245. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 107 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND MONITORING PERIQD EFFLUENT Form Approved. OMB No. 2040-0004 I YEAR. MO DAY YEAR MO DAY From 06 12 011 To 1 06 12 31 NO DISCHARGE XX NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS PH SAMPLE 12 MEASUREMENT 004D0 1 0 0 PER, EMIT 607-U '~ DAILY G~RAB EFFLUENT GOSS VALUEREQUIREMENT ~,.~~j-EFFLUENT__GROSS __VALUE MINIMUM~;s:<r$; MAXIMUM I SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 D0 PERMIT K~

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0 0 PERMIT-O 3/****~~*****~ GL DAILY CO6MPOS1 EFFLUENT GROSS VALUE REQUIRýEMENT ~.-DIYM FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 REQUREMENT REPORT REPORT -vMGD -DIY CLT EFFLUENT GROSS VALUE RUIENT MO'AVG DAýILY MX ~_ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared undermy r*A / I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel A J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Stte Vtce Prestdent information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 01 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320.1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 INjTEROFFICE qSB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 110 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved. OMB No. 2040-0004 I lMnNITnRiNIT PpFRi(,f 7 EFFLUENT YEAR ! MQ DAýYI From 01 To 06 1 12 1 31 I NO DISCHARGE I ""I NOTE: Read instructions before completing this form. ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAL'SýS TEMPERATURE, WATER DEG. SAMPLE 04*04 CENTIGRADE MEASUREMENT 04 04 00010 Z 0 0 PERMIT.. DEG C 38 3 DEG C DAILY GRAB-4 INSTREAM MONITORING

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0 0 <", PE5MITl-, MG-L -D IL GRAB*, EFFLUENT GROSS VALUE REQ.:EMENT .j:DAILY MX FLOW, IN CONDUIT OR THRU SAMPLE 03'** TREATMENT PLANT MEASUREMENT 50050 1 0 0 A PORTMITI; RP REPORT EPR MGD t7AIY ALCTD' EFFLUENT GROSS VALUE CREQUIREMEN CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1 0 0 i PERMIT 010MGIL WEEK-rLY TG RAB-4 REQUIREMENT Jn-~ 4 EFFLUENT GROSS VALUE ______DAILY: MX SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ,A TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 01 09 accurate, and complete. l am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I __information, including the possibility of fine and imprisonment for knowing violations, OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 A.INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 1 110 T F-FINAL PERMIT NUMBER I DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 YEAR M DAY YEAR MO I DAY From1 061121011 To 06 12 31 NO DISCHARGE [ ]... NOTE: Read instructions before completinct this form. NO. FREOUENcY SAMPLE PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR V SAMPLE 23 CERIODAPHNIA MEASUREMENT 23 TRP3B 1

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T-SAMPLE MEASUREMENT 11 PERMIT ~ 4 4.4 4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my A /Y i TELEPHONE DATE direct ion or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or ° persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vtce Prestdent information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 07 01 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) LTN0026450 116 G F-FINAL PERMIT NUMBER IDISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 I YEAR MO DAY YEAR MO DAY From 1 06 12 01 To 06 12 31 NO DISCHARGE [* ] NOTE: Read instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE I F ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1 / 31 VISUAL MEASUREMENT 01345 1 0 0 ,PERMIT. REPOR... PASS=0 7: SEE ViSUAL EFFLUENT GROSS VALUE REURMN~M OA AL1~PERM1IT., OIL AND GREASE VISUAL SAMPLE 0 0 1 / 31 VISUAL MEASUREMENT 94 84066 1 0 0 -~ PERMIT7~RPR YES 1 SEE. VISUAL REASUIREMENT EFFLUENT GROSS VALUE I.RQIEETM NO=0O-PERMIT'~ SAMPLE MEASUREMENT SAMPLE MEASUREMENT ýREQUIREMENT SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT ~REQUIREMENT <V, p, ~ A SAMPLE MEASUREMENT \\PýERMIT~'4 -K* REQUIREMENT'K NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE

direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted, Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer Site Vice President information, the information submitted is. to the best of my knowledge and belief, true, 423 843-6700 07 01 09 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I

information, including the possibility of fine and imprisonment for knowing violations, OFFICER OR AUTHORIZED AGENT AREA. NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NamelLocation if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 -5 2 - INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 117 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERIOD F YEAR I YEI MO I-DAY Froml 06 112 1Oll mol 06 112 131 MAJOR Form Approved. (SUBR 01) OMB No. 2040-0 F - FINAL BACKWASH EFFLUENT

- . NO DISCHARGE NOTE: Read instructions before completing this form.

004 ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCYJ SAMPLE OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1 / 31 VISUAL MEASUREMENT 01345 1 0 0 PERMIT~ .Sa..U***** RPPAS E R EEPEORT NPASST0 VI EFFLUENT GROSS VALUE REIREEN FAIL=1 _________________I MO,TOTAL. PERMIT~ OIL AND GREASE VISUAL SAMPLE 1 I 31 VISUAL MEASUREMENT 1 REQUIREMENT S UE MG~TOTALPRI SAMPLE MEASUREMENT PERMIT MRESUIREMENT i SAMPLE MEASUREMENT PERMIT N SAMPLE MEASUREMENT 'PERMIN11 ~REQUIREMENl I 4 SAMPLE MEAS UREMENT REQUIREMEN SAMPL-E MEASUREMENT REQUIREMENT,, Q NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is. to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. TELEPHONE Principal Environmental Engineer 423 843-6700 SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT AREA NUMBER CODE DATE 07 01 09 YEAR MO DAY EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 .(AINTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 118 GI F-FINAL PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER Form Approved. OMB No. 2040-0004 II KAf KIITCDIk 0C EIr n I EFFLUENT SV',P I.s S2NIN' U iNSJL SYEAR I MO I DAYj I YEAR MO DAY From1 06112101j To1 06 12 1 31

- - NO DISCHARGE c

t*o NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS OXYGEN, DISSOLVED (DO) SAMPLE 00300 1 0 0 PE'RMIT 3/4~2.0 MG/L ~ T'NIGE/ ~GRAB REQUIREMENT 19 EFLETGOSVLUE .DAILY MN' WEEK~ SOLIDS, TOTAL SUSPENDED SAMPLE

19 MEASUREMENT 19 00530 1

0 0 PERMIT 10M/ NC/ GA EFFLUENT GROSS VALUE WEEKREET> DIYM SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 25 00545 1 0 0 PERMIT'E .v

,*******i 1 0 -

.L.L dONCEJ GRB EFFLUENT GROSS VALUE REMUOREMENTH.- DAILYMX. FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 5000 0 0 ERIT 7REPORT REPORT' MGD

- ~-.OCE/.

`ESTiMA-EFFLUENT GROSS VALUE

Mi".

MOEAVG DAILY MX _____M_ BAT M.CH SAMPLE MEASUREMENT PI I REQUIREMENT SAMPLE MEASUREMENT '-PERMIT...> REQUIREMENT V SAMPLE MEASUREMENT REQUIREMENT"'" NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system.or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is.to the best of my knowledge and belief, true, PricialEnvro metalEn iner 423 843-6700_07_01 09 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ,CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions ýay be used Page 1 of I}}

ML070160095

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