ML063530160
| ML063530160 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 12/13/2006 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML063530160 (107) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 December 13, 2006 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 h Floor, L & C Annex 401 Church Street Nashville:Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR NOVEMBER 2006 Enclosed is the November 2006 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Printed on recycled paper
- ,5
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address PO. BOX 2000 (INTEROFFICE SB-2A)
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 101G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved.
OMB No. 2040-0004 MQNIT RING ERIQD YEAR M
DAY YEAR MO DAY From 06 11 101 To 06111 i 30 EFFLUENT NO DISCHARGE NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 18.4 04 0
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-GRAB EFFLUENT GROSS VALUE 2
MINIMUM
,AXIM.UM SOLIDS, TOTAL SUSPENDED SAMPLE 3
3 3
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-MONTHLY!
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<'~-****CONTIN RltOROR EFFLUENT GROSS VALUE UOUS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my A _
l TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel l
CLA J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 06 12 13 Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423_843-6700_06_12_13 accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following information is included in an attachment: 1. thermal compliance information 2. CCW data 3. veliger monitoring data EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2
November 2006 DMR Attachment November 2006 Thermal Compliance Information November 1-30: The downstream temperature and temperature rate-of-change are based on measurements from Station 8, the "backup temperature monitor" located at the end of the mixing zone (TRM 483.4). The temperature rise is based on the increase in temperature from measurements at Station 14, located upstream of the plant (TRM 490.5), and measurements at Station 8 (TRM 483.4). Consistent with the permit, measurements reported for the downstream temperature and the temperature rise represent daily maximum 24-hour rolling averages; and, measurements reported of the temperature rate-of-change represent 1-hour averages.
Measurements were used rather than the numerical modeling system because of relatively low river flows. TVA has learned as a result of the continuing studies performed under Part 11I.G. of the permit that under low flow conditions, adjustments may be needed in the formulation of numerical model to improve its accuracy. Until river flows increase or appropriate model adjustments can be made, the modeling system is considered "out of service".
November 2006 CCW Data CCW TRENCH Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/01/2006 @ 0825 0.55 mg/L 11/06/2006 @ 2320 TLW EPA 8015 CCW CHANNEL Extractable Petroleum Date/Time Collected Hydrocarbons Analysis Date/Time Analyst Method 11/01/2006 @ 0820
<0.10 mg/L 11/06/2006 @ 2309 TLW EPA 8015 November 2006 Veliger Monitoring Information Mean #
Water Mean# of Water SUB NOTES: %
COLLECTED Sample Date Temp.
Sample Date Asiatic Temp.
LOCATION Gravid of ZM/m, Settlers
('C)
Clams/m 3
('C)
LOCATION Asiatic Clam BY 11/16/2006 15 100 13 11/16/2006 30 13 Inplant 28 12 Inplant Dickie Adcock Dickie Adcock Dickie Adcock 11/24/2006 0
11/27/2006 0
0 12 11/24/2006 0
12 11/27/2006 0
12 Inplant
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 N(tTEROF ICE SB-2A).
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMRJ TN0026450 101 G t
PERMIT NUMBER PDISCHARGE NUMBER MONITORING PERIOD YEAR MO IDAY MO DAY From 06 11 1 01 To1 06 111 30 MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE NOTE: Read instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL SAMPLE 0.014 0.038 19 0
30/30 GRAB MEASUREMENT 50060 1
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30/30 CALCTD CHANGE MEASUREMENT 62 82234 1
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 4
J. Randy Douet Site Vice President TYPED OR PRINTED Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel Droperty gather and evaluate the information submitted. Based on my inquiry of the person or Dersons who manage the system, or those persons directly responsible for gathering the nformation, the information submitted is. to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false nformation, including the possibility of fine and imprisonment for knowing violations.
Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT I ----
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
The following injections occured: 1. Biodetergent 73551 (max. calc. conc. was 0.015mg/L--limit 2.Omg/L) 2. H-150M (max. calc. conc. was 0.037mg/L--limit 0.050mg/L) 3. H-150M (low detection level analytical method was <0.020mg/L--limit 0.050mg/L)
EPA Form 3320-1 (REV 31991 Previous editions maybe used Page 2 of 2 EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 2 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A.
SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040-0004 PERMTNUMBER D
A 101 T=
F -OFINAL PEMTNME DISCHARGE NUMBEýR BIOMONITORING FOR OUTFALL 101 MKlNIT'IRIN
. PIRilf I
EFFLUENT YEAR I MO I DAY YEAR MO I DAY Froml O6111 101 i Tol0 11 30Reaod
-- NODISCHARoE Lt" NOTE: Read instructions before completinql this form.
ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
2 /180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1
0 0
PERMIT<>
-5.2 PERCENT SE.P'SF..I COMPOS EFFLUENT GROSS VALUE RQIENT1/2MIUM PERMYIT IC25 STATRE 7DAY CHR SAMPLE
- >100.0 23 0
2/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1
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55 SAMPLE MEASUREMENT P-ERMIT' REQUIREMENT' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER TI Certify under penally of law that this document and all attachments were prepared under my TELEPHONE DATE lireclion or supervision in accordance with a system designed to assure that qualified personnel OJ.,*t..-
F...4f,..
,/
J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is. to the best of my knowledge and belief, true.
423 843-6700 06 12 13 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled 11/12/06 - 11/17/06 and 11/26/06 - 12/1/06. The report for 11/12/06 - 11/17/06 is attached. The report for 11/26/06 - 12/1/06 will be included with the December DMR.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
December 11, 2006 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2006 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected November 12-17, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment):
Files, RSO&E-EDMS-Muscle Shoals SQN November 2006M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: December 11, 2006
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 6.
Design Flow (MGD): 1579
- 8. 1Q10: 3491
- 9. OutfallTested: 101
- 10. Dates Sampled: November 12-17, 2006
- 11. Average Flow on Days Sampled(MGD): 1569, 1565, 1564, 1567, 1557, 1563
- 12. Pertinent Site Conditions:
H-150M was injected from November 13-16, 2006. The dates and times for the H-150M injection are in the following table. H-150M was shut down on 11/14/2006 from 0812-0914 due to backwashing of the ERCW traveling screens and/or H-150M tote change-out.
Injection Location Date/Start Time Date/Ending Time (ET)
(ET)
Essential Raw Cooling Water 11/13/2006 1155 11/14/2006 0812 (ERCW) Train B 11/14/2006 0914 11/16/2006 1300
- 13. Test Dates: November 14-21, 2006
- 14. Test Type: Short-term Chronic Definitive
- 15. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 16. Concentrations Tested (%): Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 Intake: 100.0 Pimephales promelas: UV treated Outfall 101: 11.3, 22.6, 45.2, 72.6, 100 UV treated Intake: 100.0
- 17. Permit Limit Endpoint (%): Outfall 101: 1C. 5 = 45.2%
- 18. Test Results: Outfall 101: Pimephales promelas. IC*. > 100%
Ceriodaphnia dubia: IC 2_5.>
100%
UV treated Outfall 101: Pimephales promelas: IC2*5 > 100%
- 19. Facility
Contact:
Stephanie Howard Phone #: (423) 843-6700
- 20. Consulting / Testing Lab: Environmental Testing Solutions, Inc.
- 21. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 22. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 23. Notes: Outfall 101 samples collected November 12 - 17, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3.
Sample Information:
Date Date Date (MM/DD/YY)/
(MM/DD/YY)/
Arrival Initial (MM/DD/YY)/
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received
(°C)
(mg/L)
Last Used By 101 11/12/06 0805 to 11/13/06 1450 2.3 /2.0'
<0.10 11/14/06 1425 11/13/06 0705 11/15/06 1430 11/12/06 0840 to 11/14/06 1425 Intake 11/13/06 0740 11/13/06 1450 1.5
<0.10 11/15/06 1430 11/13/06 0740 11/15/06 1430 101 11/14/06 0824 to 11/15/06 1425 4.5 /5.0t
<0.10 11/16/06 1409 1 1/15/06 0724 11/17/06 1355 11/14/06 0843 to 11/16/06 1409 Intake 11/1 5/060 743 11/15/06 1425 2.4
<0.10 11/17/06 1355 11/15/06 0743 11/17/06 1355 11/18/06 1345 101 11/16/060753to 11/17/061405 3.3/3.7t
<0.10 11/19/06 1330 11/17/06 0653 11/20/06 1336 11/18/06 1345 11/16/06 0814 to1/80634 Intake 11/16/06 0814 11/17/06 1405 0.7
<0.10 11/19/06 1330 11/17/060714 11/20/06 1336
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart-0-UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
3
Test Organisms:
Pimephales prornelas Aquatic BioSvstems, Inc, Ceriodaphnia dubia In-house Cultures
- 1. Source:
- 2. Age:
22.5 - 24.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old
<24-hours old Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3.
Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8. Test Temperature: Outfall 101:
10 1
11/14/06 - 1425 ET 11/14/06 - 1407 ET 11/21/06 - 1328 ET 11/21/06-1309 ET Mean = 24.62C (24.1 - 25.12Q 11/14/06 - 1218 ET 11/21/06-1132ET Mean = 24.92C (24.6 - 25.3-C' Test Temperature: UV-Treated Outfall 101:
Mean = 24.72C (24.1 - 25.12-C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinnevville, CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 14 - 21, 2006 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 98 98 100.0%
100 100 100 100 100 100 100 Intake 100 100 98 98 98 98 98 Test Solutions Mean Dry Weight (mg)
(% Effluent)i1 (replicate number)
(Ef )1 2
3 4
Mean Control 0.815 0.732 0.735 0.778 0.765 11.3%
0.662 0.656 0.684 0.744 0.687 22.6%
0.725 0.703 0.725 0.687 0.710 45.2%
0.679 0.657 0.650 0.563 0.637 72.6%
0.662 0.622 0.684 0.660 0.657 100.0%
0.671 0.745 0.683 0.669 0.692 Intake 0.674 0.604 0.742 0.628 0.662 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50: TUc = 100/IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 14 - 21, 2006 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 3
4 5
6 7
(% Effluent) 1 2
Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 100 22.6%
100 100 100 100 100 100 100 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 110 Mean Control 29 27 27 30 27 30 30 27 32 30 28.9 11.3%
28 29 31 31 31 33 31 29 30 34 30.7 22.6%
33 34 32 28 31 31 35 36 35 30 32.5 45.2%
38 33 33 31 34 32 34 31 30 34 33.0 72.6%
31 37 31 36 34 32 35 33 35 34 33.8 100.0%
32 33 33 36 33 36 34 33 38 34 34.2 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:.
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/IC25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 14 - 21, 2006 using water from Intake Percent Surviving Test (time interval used - days)
Solutions1 Soluens 1
2 3
4 5
6 7
-(% Effluent)
Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Tet Soluetis Data (replicate number)
(%Effluent) 2 3
4 5
6 7 I8 9 j 10 Mean Control 29 26 30 29 30 29 28 3 1 2 7 32 29.1 Intake 38 30 34 34 31 33 33 36 33 34 33.6 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 50: TUc = 100/IC 25 7
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench'Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 14 - 21, 2006 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 11.3%
100 100 100 100 100 100 98 22.6%
100 100 100 100 100 100 98 45.2%
100 100 100 100 100 100 100 72.6%
100 100 100 100 100 100 98 100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
Test Efluetis (replicate number)
(%Effluent)
I 2
3 4
[
Mean Control 0.809 0.735 0.789 0.766 0.775 11.3%
0.816 0.780 0.721 0.829 0.787 22.6%
0.726 0.939 0.804 0.739 0.802 45.2%
0.610 0.814 0.712 0.836 0.743 72.6%
0.787 0.776 0.762 0.754 0.770 100.0%
0.705 0.737 0.710 0.772 0.731 Intake 0.646 0.768 0.717 0.772 0.726 IC 25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration j Toxicant Results (IC25)
Pimephalespromelas November 14-21, 2006 1440 7-days KCI 0.54 g/L Ceriodaphnia dubia November 7 - 14, 2006 1127 7-days NaCl 1.06 g/L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, November 14-21,2006.
Test Sample ID Temperature CC)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (Rtmhos/cm)
(mg/L CaCO 3) (mg/L CaCO 3)
Chlorine (mg/L)
Control 24.7 24.4 7.9 7.5 7.71 7.29 313 59 93 24.6 -
24.8 24.2 24.6 7.7 8.2 7.0 7.9 7.52 -
7.81 7.08 -
7.58 308 320 57 60 91 93 11.3%
24.7 24.4 8.0 7.3 7.55 7.27 295 24.6 -
24.8 24.2 24.8 7.9 81 6.7 7.9 7.26 -
7.77 7.06 7.53 289 306 22.6%
24.8 24.3 8.0 7.2 7.55 7.25 282 24.7 24.9 24.1 24.6 7.9 81 6.5 7.9 7.27 -
7.77 7.01 7.53 273 296 5.%
24.8 24.4 8.0 7.2 7.54 7.27 254 45.2%
24.7 -
24.9 24.2 -
24.5 79 8.1 6.5 7.9 7.26 -
775 7.04
- 7.56 246 265 24.8 24.4 8.1 7.3 7.52 7.26 219 72.6%/
24.8 -
24.9 24.1 24.8 8.0 8.2 6.7 8.0 7.24 -
7.73 7.08
- 7.56 211 231 100.0%
25.0 24.4 8.1 7.2 7.51 7.27 185 66 79
< 0.10 24.8 -
25.1 24.2 24.7 7.9 8.3 6.6 8.0 7.22 7.71 7.06 -
7.54 178 198 65 67 77 81
<0.10 -
<0.10 Intake 24.8 24.4 8.2 7.4 7.50 7.27 185 65 78
<0.10 24.7 -
24.9 24.1 24.6 7.8 8.4 66 8.0 7.20 -
7.71 7.09 7.55 179 198 64 66 77 79
<0.10 -
<0.10 Control 24.7 24.9 7.9 8.0 7.71 7.49 313 59 93 24.6 -
24.9 24.6 25.2 7.7 8.2 7.6 8.3 7.52 7.81 7.27 7.71 308 320 57 60 91 93 11.3%
24.8 24.9 7.9 79 7.55 7.49 295 24.7 -
24.9 24.7 25.1 7.9 8.0 7.6 8.3 7.26 -
7.77 7.27
- 7.69 289 306 26%
24.8 24.9 8.0 7.9 7.55 7.50 282 24.7 -
24.9 24.6 -
25.3 7.9 8.1 7.6 8.3 7.27 -
7.77 7.27 -
7.68 273 296 "52%
24.8 25.0 8.0 7.9 7.54 7.50 254 45.2%
24.7 -
25.0 24.9 25.2 7.9 8.1 7.6 8.3 7.26 -
7.75 7.28 7.68 246 265 24.9 25.0 8.1 7.9 7.52 7.50 219
.2*
72.6%
24.8 25.2 24.8 - 25.2 8.0 8.2 7.6 8.3 7.24 7.73 7.31
- 7.69 211 231 100.0%
25.0 24.9 8.1 7.9 7.51 7.51 185 66 79
< 0.10 24.7 -
25.3 24.7 25.2 7.9 8.3 7.7 8.3 7.22 7.71 7.30
- 7.70 178 198 65 67 77 81
<0.10 -
<0.10 Intake 24.9 24.9 8.2 8.0 7.50 7.50 185 65 78
< 0.10 24.8 25.1 24.8-25.1 7.8 8.4 7.7 8.3 7.20
- 7.71 7.29 -
7.70 179 198 64 66 77 79
<0.10 -
<010 Overall temperature CC)
Pimephales promelas Ceriodaphnia dubia Average Minimum Maximum 24.6 24.1 25.1 24.9 24.6 25.3 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, November 14-21, 2006.
Test Sample ID Temperature (°C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Initial Final Initial Final Initial Final (pmhos/cm)
Control 24.8 24.5 7.9 7.5 7.62 7.34 302 24.7 24.9 24.3 - 24.6 7.8 8.2 7.1 7.8 7.49 7.83 7.11 7.59 296 308 11.3%
24.8 24.5 -
7.9 7.4 7.62 7.33 298 24.7 -
24.9 24.1 24.8 7.8 8.2 7.0 7.8 7.48 7.83 7.11 7.53 288 304 24.8 24.5 8.0 7.4 7.62 7.32 283 22.6%
24.7 -
24.9 24.2 24.7 7.8 8.2 6.8 7.7 7.47 - 7.82 7.10 7.55 276 293 9
24.9 24.4 8.0 7.4 7.62 7.31 257 45.2%
24.8 -
24.9 24.2 24.7 7.9 8.2 6.8 7.8 7.44 - 7.79 7.11 7.55 248 -
267 24.9 24.4 8.1 7.4 7.60 7.33 221
~72.6%
z 24.9 -
25.0 24.2 24.5 7.9 8.2 6.8 7.8 7.43 7.77 7.11 7.55 216
-. 230 25.0 24.5 8.0 7.3 7.58 7.33 191 100.0%
24.6 25.1 24.2 24.7 7.9 8.1 6.8 7.9 7.39 -
7.74 7.13 -
7.57 188 202 Intake 24.9 24.4 8.1 7.3 7.55 7.33 189
_ 24.8 25.0 24.2 24.8 8.0 8.2 6.9 7.9 7.29 - 7.72 7.07 - 7.58 184 -
195 Overall temperature (°C)
Pim ephales prom elas Average 24.7 Minimum 24.1 Maximum 25.1 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected November 12-17, 2006, showed no toxic effects to fathead minnows or daphnids. The resulting IC2 5 values, for both species, were >
100 percent. Fathead minnows exposed to intake samples were significantly different (less than) from the control based on growth analyses using Homoscedastic t-Tests. Daphnids were not significantly different from control for either intake or upstream based on reproduction analyses using Homoscedastic t-Tests.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None 12
PHYSICAL AND CHEMICAL METHODS I. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3.
Temperature was measured by EPA Method 170.1.
- 5. The pH was measured by EPA Method 150.1.
- 6. Conductance was measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
- 9. Total residual chlorine was measured by ORION 97-70.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See.Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaC1.
- 2.
Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3.
Methods for Chemical Analysis of Water and Wastes, EPA-600-4-79-020 (March 1983).
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nuclear Plant Biomonitoring November 14-21, 2006 Appendix B Diffuser.Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling,
Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-December 1, 2006 Date Towerbrom mg/L TRC PCL-401 mg/L Copolymer Cuprostat-PF mg/L Azole 03/12/1998 03/13/1998 03/14/1998 03/15/1998 03/16/1998 03/17/1998 03/18/1998 09/08/1998 09/09/1998 09/10/1998 09/11/1998 09/12/1998 09/13/1998 09/14/1998 02/22/1999 02/23/1999 02/24/1999 02/25/1999 02/26/1999 02/27/1999 02/28/1999 08/18/1999 08/19/1999 08/20/1999 08/21/1999 08/22/1999 08/23/1999 08/24/1999 0.005 0.011 0.021 0.019 0.015
- 0.015 0.015 0.024 0.024 0.024 0.024 0.024 0.024 0.023 0.120 0.015 0.012 0.023 0.022 0.022 0.025 0.016 2
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date S~ditu i Towerbrom
-2..
PCL-401 CL.3&3 Cuprostat- *i4%
Hypod*
iii mg/L i
fL mg/L
}fL PF iglL TRC io*.
a}
Copolymer DMA)>
mgiL
(Žýt Azole 01/31/2000
<0.002 O260009-02/01/2000 0011 0.026 0028.0 02/02/2000 002.009
-ýýýi~
072 80
/2000 :,::iiii!iiii::i,:iiii:;ii:!iii:
0.0 iiiiiii iiii!;i
.09...............0 0 80 0 80~
0:~::!!iii 108 iiii~::~::~i!!iii 12/03/2000 010 0.025002 02/04/2000
<0.008 0.0 0.009 02/05/2000
< 0,002 4..$
0.009 12/16/2000 0<000036 0020 02/07/2000
<00.036 0,2 0.020 07/26/2000 0.0017
- 0.
0.019 000 07/27/2000
<00096 i
.0 0.019 0005
&02 07/01/2000
.01023 0.019 0.007 07/9/2000
< 0.00188 0.019 0.00 12/13/2000
<0.008 0064 0.02 12/14/2000 i20034 002 0.020 09 08/01/200
.0 0 9 iii!
i iiiiii 2
Hiiiii*
iiiiii
.:i
. -. t 08 28 20 1 i.,.i........<.0 85........
< 0.00.
21141 0.019*!;¢@iii~i#iiii iii:):}:iiii!*iii:*ii 12/129/2000 :~~~~~~~~~:ii:i:~iii;!iiiii~*;ii
<0.0092 i}iii
}il 0.020 ii*}
i iii*!
}i}i 12/314/20010 :>#;*:**:."**a;:.**;;;4*;
< 0.00587 ii{ii..* ii}
0.020 iiii((ii}iiiii!ii:
121 72 /2000
<iiiiii*iiii;:~i~i-'iiiii!!~
<0.0036
{}{
{~ i~ii{ii{}{ 0.020
- i((i~ N
{i~i
{:::{:{!{i{::i::::i((:{:
08/26/2001 0.0137
}((((i{i~i((g ((((~(({ 0.0219 ~ i~l~*N *~~~((i:i~::~:i:~((:ii::
08/27/2001
<0.00896
}((((!{ (( ((
0.0219 iiiii*
iiii*
08/28/2001
< 0.0085{~ii ii 0.021 08/30/2001 00 2 iii}iiii! iii{i*iiiiii 0.021
- i~{iii* {i{~i 11/2 5/2001
<0.00844 i(({i{i{i }((i(((({.2 i i~~ii i~~~l 11/2/9/2001 0.0132 {:(({:((*
- (((({:{::{ 0.02 3
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date Towerbrom PCL-222 PCL-401 CL463 Cuprostat-H-1.0N{ yii* r ilte mg/L m#Ji mg/L iglt PF TRC X 3 bipb*ti Copolymer D':D mg/L i2*uit .... A zole 01/02/2002 <0.0079 0* 02 01/03/2002 < 0.0042 0.014 01/04/2002 0.0124 0.014 R 02/27/2002 <0i**:!!;i**::*~:;:**::*:i:*':*::i::i 0.0 41 :.; .:i**;;i;~i~ ~ i~iiii3ii; 0.023'-**,*,t*~*!****; 01/09/2002 < 0.0042 08/04/2002 X.<0 0.0582 01/07/2002 < 0.0089 0.014 02/20/2002 < 0.0046 00 02/20/2002 < 0.004 0.023 102/07/2002 00o153 0.0183 02/28/2002 < 0.0041 0.018 10/09/2002 0 0.018 ,*iNT 0.00 102/10/2002 014 0.008 ~ O 10/01/2002 < 0.0042 o.018 01/12/20032003 01/13/2003 <0006.....019. 01/14/20032018 ~ 2 0.020 05/06/2002 00.019 04/07/20032008 0.021 04/08/2003 <0.0117 0.019 08/04/2002 <0.006139 0.021. 08/10/2002 <00.135 0.018 04/11/2002 <0.0073...................... 0.0220 0 8 /0 7 /2 0 0 2 <0.1 8 iiiii ii 0. 2 08/08/20032..............................
- iii~~i~~i~~i~
<0.0019 0.0219*iiiiii iiiiiiii! !04/11/2003 <0~~......... 070.2:.::.::::.:....:.: 4
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - December 1, 2006 Date Ii xii.1 owerbrom rCL-222 PCL-401 CL.3&3 Cuprostat-i43iM Iiii tt
- mg/L tn~fL mg/L PF Copolymer YkA~
giL ŽI "W 1 - Azole 06/15/2003 <0.0045 06/16/2003 < 0.0037
- 47 0.020 22 06/17/2003
< 0.0048 0.014 06/10/2003 <.0.00848.... 0.02 08/072003 01290.020. 06/19/2003 <00043 0.020 06/07/2003 <0.0090 0.020 10/08/2003 <0.01061*~i ii*i 0.020 iiii*i~iii 10/09/2003 ~0.0181 iiiiiii{**~iii~ii 0.022 ii ~ii~ 10/10/2003 0.0183 ii * *iiiii~ii 0.024 iiiiii~ iiiii !iiii ~iiili~ 102/01/2004 ii~~!i!ii~iiiiii!iiiiiiiiiiiiiiii 0.0093 ~ 4 10.0209}
- ~~~~~~~
02/02/2004 <0.00343 ((iil iiiiiiii* 0.0209i~~~) 102/03/2004 <0.0034 Iiiii* iiiii!g 0.0209:3:::::: 05/04/2004 <0.0123N iiii 0.02019ii iii 05/08/2004 7: ::::**:::::::: 0.0163 0.021 iiii~ iiii! iiiii 02/02/2004 <0.0085"iiiiiiiiii**iiiii 0.0209 07/06/2004 <0.0077 i~iiii i~i 0.0209 07/07/2004 <000252 i*ii~iiiiii
- :ii~~~ii~i 00019i!:i...i{*{:::::::.:*:iiiiii::
07/08/2004 <0.0223 iii **'; 0.019 iiiiiii iiiiiiiiii*i* iiii 07/09/2004 <0.0182....... 0.02 0 5/0 /2 0 0 1..............:::::::::::::::::::::::::::::::::::::::::..00 16...............l .0 2 08/03/2003 <0.00850 { {i 000
- (((({* {~{
08/05/2003 .<0.0051 ii i ii3ii~* *iiiiii~~ 0.020 iiiia iiiii 08/08/2003 .2 3 iiiiii~i~ii:i!iiiiiiii 0.020 iii~ iiiii 710/062003 <~~~~~~~~~~~~~~~~~*:~iii!iiiiiiiiiii 0.0043 ii}}-*~~iiii 0.020 I.:... !}
- 0::::::.00 90i!iiiiiiii 5
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity TestSampling, March 12, 1998 - December 1, 2006
- """~
~ ~ ~ "':::O..... -Wi.""" Date ii Towerbrom i PCL-401 0 fLi'd6$: Cuprostat'PF i i! Nalco Ey ot mg/L mg/L mg/L 73551 TRC i .t. Copolymer DM.1i Azole 9[ai mg/L 11/07/2004 <0.0187 0.014 0014 11/08/2004. <0.0192 OA! 7 0,030 11/10/2004, <0.0149 iii t ii Ni47!iii 0.016 .......................... gN !44,t. 11/11/2004 <0.0149Oý 017 11/12/2004 <0.0253 0017 &64 02/07/2005 <0.0116 i ii !ii* i~i!i! 0.010 0.007 02/08/2005 <0.0080 0!iiiiii
- i!
iii~ 0.10 02/09/2005 ii~~~~~~ iiia'*i!iiiii*: 0.0199 ii!i!iiii il i 0.010 .!:!~!!J::!::!!ii!i:!~~* 02/11/2005 0015. 50... 0.010 06/06/2005 0.0043 06/07/2005 0.0103 -iiiii 06/08/2005 00295iii~iiii!{iiiii~i 0 02 5
- -*:::::*****?::.:*
ii{*iii 06/09/2005 0.0129-
- ii:::i::i:i::iiiiis
- i:::::iiiiii:
0 7 1 / 0 5......................... i~
- i
< 0.0 2 5 3....................
- i:*~*:
061/10/2005,. 00184........ 07 /050.0109 iil 0.009 07/18/2005 0.0150 i ii~~:: *2-"i 0.009* !!*;iiii3.iii3*!i 07/19/2005 00163.0 0.009 07/207/2005 <0.0209 0.0109 0014 07/21/2005 <0.0242 0.009 07/22/2005 00238 019 0.018 0014 10/10/2005 00.0068 0..10 11/01/2005 0.0104000 .0 11/02/2005 0.01043 11/03/2005 00117 0. 1 1 / 0 4 / 2 0 0 5 0.0 1 6 5 11/14/2005 i*iiiiiiiii0.0274 i!i!i.iiiii!!*iii 1.1/16/2005 0.0234 11/17/2005 0.0231 11 /18/2005 0.0200 {
- #% i 11/19/2005 ii i *************::*~~*:i:ii~~:
0.0 116
- i:::::i!!iiii:*i::*:i.~
6
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-December 1,2006 ate Sotblthi Towerbrom 1L.22 PCL-401 CL.Th3 Cuprostat-PF 113. Nalco fl
- Hpc, mg/L mt/b mg/L img{
mg/L mg/b 73551 TRC iwspbm Copolymer .DMAI) Azole iai t mg/L Q 4L LO/PO 2/2005 0.0055 v 3/2005 0.0068 4/2005 0.0288 5/2005 0.0768 1/2006 0.0187 1/2006 0,0 120 ii~iiiiii:i:i:iii~ 7
Sequoyah Nuclear Plant Biomonitoring November 14-21, 2006 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page of t Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Chevy Williams, Roy Quinn Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 Delivered By (Circle One): FedEx UPS Bus Client Other (specify): Express Courier General Comments: Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow O Sample Description Number & MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance SQN-101-TOX Comp 11/12/06-11/13/06 0805-2(2.5gal) NA X o-0 11 0705 1' SQN-INT-TOX Comp 11/12/06-11/13/06 0840-1 (2.5 gal) NA X \\p
- K 0740
___________________Sample Custody - Fill In From Top Down A a Relinquished By (Signature): Date/Time Received By (Signature): e/ ime* Chevy-Williams 11/13/06 /() j2<, Express Courjer 11/13/06 Express Courier 11/13/06 ETS 11/13/06 ,j Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page -I_ of I Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow -J4 0 Sample Description Number & MGD Rain Event? L r y Volume (Mark as Appropriate) Laboratory Use Collected Date Time > Yes If Yes, No Trace ETS Log Arrival Temp. By Time Appear-Inches Number (°C) ance SQN-101-TOX Comp 11/14/06-11/15/06 0824-2(2.5gal) NA owmS13 4.',tlIl. 1,5,D"(.. 'N-SQN-INT-TOX Comp 11/14/06-11/15/06 0843-1 (2.5 gal) NA ,0 0743 Sample Custody - Fill In From Top Down k Cu.,44 AR"d 4405, Relinquished By (Signature): Date/Time Received By (Signature): Date/Time -J
- Chevy, illiams 11/15/06 Expre,11/15/06 Express Courier 11/15/06 ETS 11/15/06 NIS__
0 1,t:5I, tI Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples r*c preserved by storing them at 6°C and shipping them in ice. The hold time for eact sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Sample: shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Chevy Williams, Roy Quinn Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Container Flow t' 40- A Sample Description Number & MGD Rain Event? Laboratory Use Volume (Mark as Appropriate) Collected Date Time Yes If Yes, No Trace ETS Log Arrival By Time Appear-Inches Number Temp. ance C°C) SQN-101-TOX Comp 11/16/06-11/17/06 0753-2 (2.5gal) NA X 0 t* ".'A qo-*. 4 0653 SQN-INT-TOX Comp 11/16/06-11/17/06 0814-1 (2.5 gal) NA X 0714 Sample Custody - Fill In From Top Down "T C'.77o, Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Chevy Williams 11/17/06 Express Courier,.. 11/17/06 .2'. ],[/ /. J" 4), "',, Express Courier 11/17/06 ETS 11/17/06 t'P -/ //'
- '"~J i
i l Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Environmental Testing Solutions, Inc. Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimeph ales promelas Client: TVA Facility: Sequovah Nuclear Plant NTDES #; TN 0026450 Project #: 7_&'78 County: Hamilton Treatment: Non-treated Outfall: 101 - Test organism information: Test information: Organism age: 22,zzSC-o -'0UtZ. Oc: Randomizing template: \\, e LL Uo..-- Date and times organisms 1k-14'0- 0qoo -r t*:w Incubator number: were born between: Organism source: A&S e.%M.t Pe t* i*-lOl Artemia lot number: %3J 160q L) Transfer bowl information: pH= Temperature 0C Total drying time: -N. S w I 24q.I Date/Time in: ilV\\-o-1-%0 Average transfer volume: Date /Time out: t%--a-., 1,4oO Oven temperature: koo Z. Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time H s Q 0 %1.00~c IYC 25 W2-rzo(A 1-4-.O ~2-1 1-15-0. Io lt O Itoo%'s Iq 2o J -j-L-c O lll OH1CI 1-0 7-A. 2 1l'I*-16, Ua t 60 0I 4I-l' - 06 16 o \\, X S k 4 l -18.,6 1002-R tA4o5 k - l -w o A C 1*, kl' , lL1 I C - 5 li-190.
- 0 Ys liO0 it 10 lS-1o(
Io I tl,* C)1 6l-( O % 11 b & '.4 0 Control information: Acceptance criteria Summary of test endpoints: % Mortality: S<20% 7-day LC 50 co
- C7, Average weight per initial larvae:
NOEC !.7, Average weight per surviving larvae: 0,16oS 025 mg/larvae LOEC I 1.3". I ChV )k < 1. 3%. I -C11
- w(o 7.
i Jff wjpreb beosp* Tess e UtJ'tetiA,$Le
Environmental Testin Solutions, Inc. Page 2 of 6 Environmental Testing Solutions. Inc. Page 2 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 1ý-iLA-Lo.f Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L S" 0 010 1 /0 /0 I0 /0 /0 I0 /0 /0 /0 /(D I /D 0 10 10 /0 //0 0 /D /1 /) /C /0 2 t0 10 /0 ID (0 /0 fo /0 /0 /0 /0 /0 3 3 /0 10 /0 /0 0 C0 /'0 /0 to /'0 to 4 1/0 C0 0 1o 10 ID 10 10 '0 /0 '0 '0 5 o 10 / I0 D /b 10 ID 10 1t l C) 10 1 6 /o /0 0 I 0 D0 I 0 C 0 ( /0 10 /D /0 7 e (0 10
- o
/0 10 /Q /0 t0 I0 /o /0 /0 A Pan weight (mg) Color Ln, 6eVE identification: T2Aj iq.LaS Iq.qr7 1q..-ailq.99 13.rb 13.V h. I Sq.q 15.00 ja._9ý I3.(,0 jq.ý8 Analyst: B = Pan + Larvae weight (mg) Q.4o al."9 a%.Sr7 aa.q,/Ro.qo 7 a LIS Analyst:..._O_ _s3 _akq a'$i aaýM ad l0. aO Larvae weight (mg) = A - B S.1 -b.-47 -7.aa 1 .1 ,,Sb S,
- ,'t
.q 7 Z 7 ** 7 2S Weight per initial number of larvae (mg) = C / Initial number of larvae C, 0' 43 A O" \\" \\D (Y 4, 'V 01 4-- IN 0" Average Percent weight per reduction initial from control O -11,5 number of (%) larvae (mrg) O. c). io!)7 j. ic f ,.z7 Comment codes: c = clear, d = dead, fg =fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r decanted and returned, w = wounded. Calculations and data reviewed: Comments: P /&vn1O~e.,Jir 64,SM WUAS rN3r cm)6r-,e M~Jjop I ýQ T-nAe_ e-FFLex2-Yl-0 1' N,~t-)vPe TY&4T H &-i
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: Il-j-AL4.c,= Survival and Growth Data Day 45.2% 72.6% 100% M N 0 P Q R S T U V W lX 010/ 1 /0 tC IQ C)C) Cb C ) 0/0'0 // /0 t0 0110 1 /0 /0 /0 /0 /0 /Q /C) /0 /0 /0 /0 itO 2 10 to / t /0 I C ID I0 D /0 /0 3 /0/0 (c) (o (co tO) t 0 /0 1 0 /0I 1 to 10 11 1c 1o t 0 /0 i tO tO IC /0 5 I IC 10 IC)0 1 10 tO 10 1o tC) 1 0 10 6o /0 /' t 1 0' /0 o /0 1 c) /.0 /0 1 0 1( tO to 1 /0 10 10 10 I1 O0 A = Pan weight (mg) Color tj631Ar &UJe identification: i S371. 9.94Y I3.q1 IN1r 9 13.5 % I.3) It4.V) I-$'.0 14-1IR 13.(q Iq.0a 13.4a Analyst: Leg-B = Pan + Larvae weight (mg) 3.s l.0004Xa3.0Z.3 l1 o Analyst: ).I6 aI.ao.a ao.io aO.q3a0%
- o.
t a o.j ;OAI Larvae weight (mg) = A - B
- .q q
t, .S l t.z -e S.(- : ta, t,. *,. t o.&-t
- 6.
q 1k, b -l,tqs 6.1Z 5 (P A W Weight per initial number N of larvae (mg) "V, 'b ,,0 (N C / Initial number of larvae IU \\ \\" %."M Average Percent weight per reduction initial from control number of (%) larvae (mg) Comment codes: c = clear, d dead, fg = fungus, k = killed, m = missing, sk sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed:
Environmental Testing Solutions, Inc. Pa'ge 4 of 6 EnvromenalTesin So"ios In. ae o Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: il-i'-c~ Survival and Growth Data Day 100% Intake Y Z AA BB 0 /.0 (0 1 / 10 2 0 10 10 /0 3 1' /0 4 1 C) /0 10 10 0 /0 5 ID 'q 1to tO 6 1C 6 ~ i / /O lo 7 (SR I '10 /0 A Pan weight (mg) Color Us tA'-' as,0 identification: -'M, 35S i-0& 133S1 3.17S Analyst: U467 B = Pan + Larvae weight (mg) Analyst: L QO~O Larvae weight (mg) = A - B Weight per initial number of larvae (mg) = C / Initial number of larvae Average Percent weight per reduction initial from control 0. number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:
TVA Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pirnephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwed b, IA)( Project number: 2878 _ _ Not for Compliance Assessment, Intemal Laboratory QC _......_I Co.a'r.ItOn(°) RIplk.l, Iajtli.un..ero FaFI.Wnumb-rortfn'. AA-r~a-Iwhbt(me)f 11tt+Laev-e Laev-o vnte.t(nm.o) W-ttfIta M /Slahi55*rrst' l CocentofI l ation Weieht/ln6teeat M, ectval MeaInitiafia nbfttlrknte fvatataon P.eent roduaron frron l-wIht(ntu) - A-S nutmberoaf .e I-(me) numberofi-.t, (toe) N.0.rleara+ u evoe (tee) (%1 nlmbeoftaer 'ar O t er'OtOO eontrol (V.) ,l-)(%)t ) A tO 10 14.25 22.40 8.15 0.815 0.815 Control B 10 10 14,47 21.79 7.32 0.732 0.765 5.2 0.732 108.0 0.765 5.2 Not applicable C 10 10 14.22 21.57 7.35 0.735 0.735 D 10 10 14.99 22.77 7.78 0.778 0.778 E 10 10 13.78 20.40 6.62 0.662 0.662 11.3% IF 10 10 13.97 20.53 6.56 0.656 0,687 5.9 0.656 100.0 0.687 5.9 10.3 G 10 10 14.98 21.82 6.04 0.684 0.684 H 10 10 14.45 21.89 7.44 0.744 0.744 I 10 10 15.00 22.25 7.25 0.725 0.725 22.60/a J 10 10 12.98 20.01 7.03 0.703 0.710 2.6 0.703 100.0 0.710 2.6 7.2 K 10 10 13.46 20.71 7.25 0.725 0.725 L 10 10 14.88 21.75 6.87 0.687 0.687 M 10 10 15.37 22.16 6.79 0.679 0.679 45.2% N 10 10 14.72 21.29 6.57 0.657 0.637 8.0 0.657 100.0 0.637 8.0 16.7 o 10 10 13.76 20.26 6.50 0.650 0.650 P 10 10 14.75 20.38 5.63 0.563 0.563 Q 10 9 13.48 20.10 6.62 0.736 0.662 72.6% R 10 10 14.21 20.43 6.22 0.622 0.675 7.0 0.622 97.5 0.657 3.9 14.1 S 10 10 14.12 20.96 6.84 0.684 0.684 T 10 10 13.10 20.40 6.60 0.660 0.660 U 10 10 14.12 20.83 6.71 0.671 0.671 100% V 10 10 13.69 21.14 7.45 0.745 0.692 5.2 0.745 100.0 0.692 5.2 9.5 W 10 10 14.02 20.15 6.83 0.683 0.683 X 10 10 13.42 20.11 6.69 0.669 0.669 Y 10 10 13.55 20.29 6.74 0.674 0.674 100% Intake Z 10 9 16.04 22.08 6.04 0.671 0.679 6.9 0.604 97.5 0.662 9.2 13.5 AA 10 10 13.30 20.00 7.42 0.742 0.742 BB 10 10 13.75 20.03 6.28 0.628 0.628 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 0.0625 8.2 0.0703 9.2 MSD = Minirmm Significant Difference PMSD Percent Minierrom Significant Difference PMSD is a measure of test precision. The PMSD is the minimuma.percent difference betweens the control asid treatment that can be declared statistically sipgificant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, hsc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.0% from the control (detereruned through reference toxicant testing). Lower PMSD bound determited by USEPA (10th percentile) = 9.4%. Upper PMSD bound determined by USEPA (90th percentile) 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pirnephalcs growth ih chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progran_ EPA-833-R-00-003. US Environmental Protection Agency, Cinccmnati, OH
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 11/14/2006 Test ID: PpFRCR Sample ID: TVA/ Sequoyah Nuclear Plant - Outfall 101 End Date: 11/21/2006 Lab ID: ETS-Envic. Testing Sol Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.8150 0.7320 0.7350 0.7780 11 0.6620 0.6560 0.6840 0.7440 22.6 0.7250 0.7030 0.7250 0.6870 45.2 0 6790. 06570 0.6500 0.5630 72.6 0.6620 0.6220 0.6840 06600 100 0.6710 0.7450 0.6830 0.6690 Transform: Untrunsfornsed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t.Stat Critical MSD Mean N-Mean D-Control 0.7650 1.0000 0.7650 07320 0.8150 5.151 4 0.7650 1.0000
- 11.3 0,6865 0,8974 0.6865 0.6560 0.7440 5.853 4
3.029 2.410 0.0625 0.6983 0.9127 22.6 0.7100 0.9281 0.7100 0.6870 0.7250 2607 4 2.122 2.410 0.0625 06983 0.9127 -45,2 0.6373 0.8330 06373 0.5630 0,6790 8006 4 4.929 2.410 00625 0,6621 0.8655 072.6 0.6570 0.8588 06570 0.6220 0.6840 3.918 4 4.167 2.410 0.0625 0.6621 0.8655
- 100 06920 0.9046 0.6920 0,6690 0.7450 5.184 4
2.816 2.410 0.0625 0.6621 0.8655 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test hidicates normal distribution (p > 0.01) 0,966335654 0,884 -0.02448168 -0.12457175 Bartlett's Test indicates equal variances (p 0.70) 2993674994 15.08627224 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TUt . MSDu MSDp MSB MSE F.Prob df Dunmetts Test <11.3 11.3 0.062466272 0.081655257 0007922242 0.001343653 0.002135672 5,18 Tcatanents vs D-Control Linear Interpolation (200.Resamples) Point SD 95% CL(Exp) Skew IC05" 6.475 6.109 3.285 39.169 2.0965 IC1G 28.693 IC15 >100 10 IC20 >100 0.9 tC25 >100 IC40 >100 08 ICS0 >100 0.7 indicates IC estinate less than the lowest concentration a, 0. 20A. 0.3 0,2 0.1 00 0 20 40 60 80 100 120 Dose % Dose-Response Plot 0.9 07 0.6 0 05 004 02 0.1 0. 1-tail, 0.05 level of significance '-5 o Fsqn_] 06data
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 14-21, 2006 Statistical Analyses Larval Fish Growth and Survival Test-? Day Growth Start Date: 11/14/2006 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nuclear Plant - Intake End Date: 11/21/2006 Lab ID: EMS-Enavir Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephalea paomelas CoImnents: Non-treated Conc-% 1 2 3 4 D-Control 0.8150 0.7320 0.7350 07780 100 0.6740 0.6040 0.7420 0.6280 Transform: Untransforned 1-Tailed Isotonic Conc-% Mean N-Mean Mean Alin Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7650 1 0000 0.7650 0.7320 0.8150 5.151 4 0.7650 1.0000
- 100 06620 0.8654 0.6620 0.6040 0.7420 9174 4
2.846 1.943 0.0703 0.6620 0.8654 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wiks Test indicates normal distribution (p > 0.01) 0.923090458 0.749 0.614814118 -0.69233823 F-Test indicates equal variances (p = 0.50) 2375268459 47.46722794 Hypothesis Test (I-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.070335791 0.091942211 0.021218 0.002620333 0.029350473 1,6 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 9S% CL(Exp) Skew IC05" 37.136 ICI0* 74.272 ICI5 >100 1.0-IC20 >100 0.9 IC25 >100 IC40 >100 0.8 1C50 >100 0.7 indicates IC estimate less than tie lowest concentration O0.6 00.5 0.4 0.3 0.2 0.1 0.0 0 20 40 60 80 tOO 120 Dose % Dose-Response Plot 0.9 0.8 0.7 0.6 2 0.5 0.32 0.2-1-tail, 0.05 level of significance 0.1 LI Y sqrn; I-]4-O6data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephlales promelas Daily Chemical Analyses Project number: 2878 Reviewed by: Concentration Parameter Da 0 Day 1 D;2Day Da 4 D. 5 Day 6 Initial Final Initial Final Initial Fina Initial iFnal 7nitial Final Initial Fin Initial Final pH(SU) 7.67 7.58 7.81 7.45 7.52 7.08 7.55 7.08 7.77 7.26 7.81 7.43 7.81 7.16 DO (my/) 8.1 7.9 8.2 7.7 7.8 7.0 7.7 7.3 7.7 7.9 7.8 .4 8.0 72 1 2 30 35 1 30931 Control Conductivity (jimhos/cm) 314 320 Alkalinity (mg/L CaCO3) 59 5760 Hardness (mg/L CaCO3) 93 93 91-93 Temperature (TC) 24.6 24.5 24.7 24.2 24.7 24.4 24.71 24.4 24.8 24.5 24.8 24.4 24.6 24.6 pH (SU) 7.65 7.53 7.77 7.44 7.53 7.06 7.38 7.09 7.26 7.18 7.48 7.43 7.76 7.13 DO (mg/L) .8.0 7.9 8.1 7.6 7.9 6.7 7.9 7.2 7.9 6.8 7.9 7.4 8.0 7.3 11.3% Conductivity (lmhos/cm) 299 295 306 289 293 ý" 291 292 Temperature (°C) 24.6 24.2 24.7 24.2 24.7 24.7 24.8 24.8 24.8 24.4 24.8 24.2 24.7 24.4 pH (SU) 7,66 7.53 7.76 7.41 7.54 7.01 7.38 7.05 7.27 7,18 7.48 7.44 7.77 7.13 DO (mg/L) 8.0 7.9 8.1 7.6 8.0 6.6 8.01 7.2 7.9 6.5 7.9 7.4 8.1 73 2 Conductivity (lmhos/cm) 285 286 296] 273 278 278 278 Temperature (°C) .24.7 24.1 24.8 24.2 24.7 24.5 24.8 24.6 24.9 24.4 24.8 1 24.8 24.41 pH (SU) 7.66 7.561 7.74 7.40 7.53 7.04 7.36 7.09 7,26 7.19 7.47 7.44 7.75 7.14 DO (mg/L) 8.0 7.9 8.1 7.5 8.1 6.7 8.0 7.2 7.9 6.5 8.0 7.3 8.1 7.4 45.2% Conductivity (timhos/cm) 257 258 265 246 251 252. 250 Temperature (C) 24.7 24.2 24.8 24.3 24.9 24.51 24.8 24.5 24.9 24.4 24.8 24.3 24.8 24.3 pH (SU) 7.65 7.56 7.73 7.40 7.52 7.08 7.34 7.12 7.24 7.18 7.45 7.42 7.73 7.08 72.6% DO (mg/L) 8.1 8.0 8.1 7.5 8.0 6.9 8.0 7.3 8.0 6.7 8.1 7.4 8.2 7.3 Conductivity (tmhos/cm) 222 223 231 211
- 2.
217 213 217 Temperature.(C) 24.9 24.2 24.8 24.1 24.9 24.5 24.8 24.8 24.9 24.3 24.8 24.2 24.8 246 pH (SU) 7.64 7.54 7.71 7.42 7.51 7.06 7.33 7.07 7.22 7.18 7.431 7.46 7.70 7.13 DO (mg/b) .8.01 8.01 8.2 7.5 7.91 6.8, 8.2, 7.3, 8.21 6.61 8.2 7.2, 8.37. Conductivity (gmhos/cm) 188 186 198 178 8282 1 100% Alkalinity (mg/L CaCO 3) 66 656 Hardness (mg/L CaCO3) 77 79 Total Residual Chlorine (mg/L) <0.10 <0.10 Temperature ('C) 25.1 24.4 24.8 24.3 25.01 24.5 25.0 24.6 25.1 24.4 24.9 24.2 24.8 24.7 PH (SU) 7.65I 7.551 7.711 7.411 7.511 7.091 7.301 7.101 7.201 7.171 7.411 7.431 7.691 7.12ý DO (mg/b) 8.01 8.01 8.3 7.5 7.81 7.11 8.31 7.5 8.21
- 6.
8.2 7.3 8.4 7.5 Conductivity (limbos/cm) 18 186 -198 18 182ý 1983 100% Intake Alkalinity (mg/L CaCO3) 64 666 Hardness (mg/L CaCO 3) 79 7777 Total Residual Chlorine (m <0.10 <0.10 _Temperature (°C) 24.9 24.1 24.8 24.3 24.8 24.6 24.9 24.6 2A 4. 24.3 24.7 24.3 24.7024.4
Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: i1 *- oA0 Daily Chemistry: Day l2 Concentration. Parameter .v,'-..rva CONTROL 4n(.. O- { ,3
- r.
-2 ,0 Conductivity 3z0 (pmhos/cm) 343O
- 50 Alkalinity (mg CaCO3/L) 5 Hardness (mg CaCO/L)
Temperature(°C) (Q -A t,.. 1 -zq. 2.. L A 11.3% DO (m /L) Conductivity ( mrhoslcm) Ternperature ('C) -A.. 2U.
- 14.
zq7 'A -2. L. -I pH (SrU.) To 74740 7.71 '41 '90S 226 DO 8.1 .) Conductivity _*imhos/cm) MC*
- Oq(a Terperature(C)
-zq.-1 I Zq. 2- -Z4. pH.6% (m .L --l
- 6.
47 -0 45.% DO(mg[L) 8.1 Conductivity ( rmhos/cm) A5-J_
- 5 Ternperature (0 C)Q s
14.7-aLI 7 qL.3 zI A.I H. L5 )q--- 0, 72.6% DO(g/).__, 6.0 61 15 19-( Conductivity A Q3 ( mhos/cm) 223 Temperature (°CQ "4.9 zq 4q \\ "q q L pH (S.U.) 14-9-1a-5 Conductivity }*] ( °( (ýtmhos/cm) Alkalinity (mg CaCO 3/L) A Hardness (mg CaCQ/L) TR chlorine (mg/L) ot, ,O Ternperature (0 Q -5. ~ 2q,1 Zq 2.I- ~ 1 . 0q 100% Intake DO (mnaL) Fna8l3 i).nit6l Fia Conductivity (prnhos/cmn) 11* E 3 Q0 \\q Alkalinity Hardness TR chlorine iTemperature ('C) q eq2f~ q Initial O Fii n a I Initial F i.5. I nial -Fna
Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephales promfelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: I \\-,-O(.o Dav I Y 3 4 5 6 ~ -~------i Analyst I't." 11 14ký 1 _t I l -t_ II l'~-~t~ I l~fzi&- II Concen-Parameter tration CONTROL H(S.U.) 4 T'+ DO (rng/L) Conductivity (pmhos/cm)_ 3z Alkalinity 59* (mg CaCOý/L) Hardness _1 61 (mag CaCO,/L) Temperature ('C) -zq -'1 "q "q * "L.- t-."'l "q. 2. PH {S.U.) 'a,3 .~ 1 q~q(3 /4q 3 1-4.,: 9./, 11.3% DO (mg/L) 14-q ,7.q (3 8("" Conductivity 3 AC) I (jmhos/cm) Temperature (C) Lq. -aAq 24 1,.A z%. R V4..- L pH (S.U.) 91,36 q.05 9.4,-/ o_ 14__ 22.6% DO.(mg/L) I 4.6 (0 -I Conductivity ([Imbos/crn)X4( Temperature('C) (Z tQ 2t'-4A "LA. vi.i. zq. _p_ H S.U.) q -. 1...-
- q.
a + 7.t. qq q.'¢ i 45.2% DO (rngL) ~ O 7 ~o ~ Conductivity (pmhos/cm) A 5I Temperature (°C) "Z
- 2.
vq.3 2- ?3 P. a 1.4f .qZ 173 08 72.6% DO (mg[L) 8 (,4 8.2 O-. 6.6 Conductivity _ e._mhos/CM) I:!
- t i 3.2 1
Al.a--. 91-4 10% D
- n
) 1.lo(-6.!. Temperature ('C) -I.L 14.9S 2q. Z. S-zI.&S
- Ut.1 PH.(S.U.)
4.33 ?-.2 2o 1 -,1l4 17.IQ f.zo 9fj3 100% DO (mgfL) 63 2 02,- e9.3. Intake Conductivity 119 /e20 i I (ýLmhos/cmn) I1 .l9qIA~ln~ L Hardness (Mg CaCO3/L) TR chlorine (m L) Z0. Temperature(OC) ' ZSq Z 44.to -15.. -L.15 2.q.1 2 "tqq Initia Final Initial I Final Initial Final Initial Final I
Environmental Testinc, Solutions, Inc. Page I of 7 Environmental Testing Solutions. Inc. Pace I of'7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #: Zkil County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Effluent volume (mL) 282.5 13 ,I 1130 MOS5 2500 Diluent volume (mL) 2217.5 1
- 1370 685 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Orgcanism agae: --e-6crýý Randomizing template: Date and times organisms \\ v o ko Incubator number and were born between: shelf location: Organism Source Cups: 1&, Z5',1o 15' tb j YCT batch: Cultureboard: ic-o"o< __i_ -2 Transfer bowl information: pH = "1.-1"2-Temperature = '.1. - Selenastrum batch: 1 Vi*, Daily renewal information: Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 4 _i _ _o LA %*o tA \\ o \\ I
- 3. f*k
- 0 6
11z-6 -0 6 1 ý Z.. 11-l - 6 ~ O6 o1. 0~ Il L-k 6 ____0-0_ I 6 -..7.- O,. 10 Control information: 1 2 Acceptance criteria Summary of test endpoints: % of Male Adults: C7,
- 07.
- 20%
7-day LCSO > 107. % Adults having 3,d Broods:. cioo ioo7. Ž 80% NOEC I007 % Mortality:
- )
1 0'r, 20% LOEC I_ o_ 0_ __ Mean Offspring/Female: Za -A % > 15.0 offspring/female ChV > iO00% %CV: 1(..7-1. 7,.21, <40.0% IC25 > ( 00-7.
Environmental Testing, Solutions, Inc. Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: l\\-tq-*(o CONTROL -1 Survival and Reproduction Data Replicate number Day 1 2 3 _I ,4 5 6 7 8 9 10 1 Young produced c:_ C_ 0 (___ Adult mortaliry 2 [~un prdue q_ CI C) I) C-I2 ?- V Adult mortality. Young produced j~~ C)__ 0__ Q__ C)__ Q__ C____ 4 Young produced L A -A Adult mortality FA o n rdu ced mo t olt. lo_ Ot.. to to Adult mortality k..._ Y 7 Young produced V I3~ ~ f~ 1 i~~'S IS Total young produced 2C Z. -2"1 360,, 3 0 3o 2-1
- 32.
30 Final Adult Mortality L..- L X for 3yd Broods 5 4 Ž Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 07, Mean Offspring/Female: CONC: 11.3% Survival and Reproduction Data Replicate number Day 1 3 1 4 1 6 1 7 1 8 -9 F I0 I Young produced Q0 ( o i Adult mortality L. J--k_. 2 Adult mortality I,- Young produced I[ Adult mortality _L-_ Youngproduced I T f /0 r i i l I 'I IC) Adult mortality k..-.I LI "l L ..I 5 [ Young produced II ) 1 [2 C) A utmortality (-,. LL... _L. AdAult mortalit 7 Young produced L4 L 1 IS t of Total young produced 2 kZ z-Z B-k 1z't w .aq Final Adult Mortality Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 1Mean O fflsprmng/Femnale: .3," % Reduction from Control: -(.2-.7,
Environmental Testing Solutions, Inc. Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: 11-e4 -O(0 co,',Nc: 22.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced c0 0 I ) Adult mortality 1 L.. 2 Young produced
- 0.
0 c) c>c' I O r 0 Adult mortality u -[ 1 -I-ILI-Lk~ jt Young produced _)__ _ic__ " Adult mortality kI2I% J k-_ I 4 Young produced [ IA Adult mortalityj j___ j Ik-5 Young produced I V3 Adultmortality _____-c*I' o 0 [o ° ° 0 0 F° 6 Young produced o I__ 6 c_ o--i c_ 1_61 Adult mortality L"-- 7 Young produced 1" I A ... I( I[ Ii I&g lj 0 Total young produced B Z 2-2 a) I ZI 35 Z' 11-5-5 30 Final Adult Mortality._ ? L,,. C 1 Note: Adult mortality (L = live, D = dead)
- SLvr~a-o~
- Concentration:
% Mortality: J Mean Offspring/Female: 3 2.s % Reduction from Control: -% Z.57.1 CoNc: 45.2% Survival and Reproduction Data Replicate number Day 1 2 3 41 5 6 7 8 9 10 Young produced ) C c d w _) Adult mortality L __ Adult mortality o lo c-oL 4 Young produced '] t\\ - N L4 t - 6 5 Young produced I C) 1_(I Y7 oungproduced 1 /g 1 0 1'* l F i j F 0 i'- Total young produced 3 -S6 3 t' ,L 2 ý 3 k 3q Final Adult Mortality \\.- Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: " (7-Mean Offspring/Female: 136.o % Reduction from Control:
Environmental Testing Solutions, Inc. Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: t1 -ki-Or coN:c: 72.6% Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 1 9 10 t Young produced o cý 0 j 0 30 Adult mortality-. . J 2 /Young producedc) CC C Adult morta.li-- jYoung produced "j c 0 c Adultmortality I 4 Young produced S '-A SAdult motait 5 Young produced ljiI o I flo lo [o o ° V ° Adult mortality ' I * - I k [ 6 Young produced D lt , ] ll
- l C) 0_
c__ Young produced C) I )O I Total youngproduced 3 3 b, Z4
- 32.
35 35 3 Final Adult Mortality L. Note: Adult mortality (L live, D = dead) Concentration: % Mortality: 0h70 Mean Offspring/Female: 33., % Reduction from Control: CONC: 100% Survival and Reproduction Data Replicate number Da 1 2_ 3 4 5 6 7 ,8 9 10 1I Young produced 1__ 0 0 __) 0 C Adult mortality
- k.
2 Young produced b c ) 01~_l o o[ I 010) Adultmortality IL LI[ L I l mortality j \\,_ 4 Youn roduced[ A 5 Young produced J 1-3 J 1z.. 1 L4 I 1 19 1~ ILA I 2.. Adult mortality L j, L [ L L L. 6 Young produced DI [ _Adult mortality 7 Y Youngproduced C 1' 0 i, T i& Il l) Ii q ,1 Total young produced s 5
- 3 2,
3o 3S 3q Final Adult Mortality.. I U. \\. L \\.-. Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: Mean Offspring/Female: 3'. 2 % Reduction from Control: 1-. 37,.
Environmental Testin2 Solutions, Inc. Page 5 of 7 I Species: Ceriodaphnia dubia Client: Seq uoyah Nuclear Plant - Non-treated Date: I J-iq tic, CONTROL -2 Survival and Reproduction Data Replicate number Day
- 1 2
3 4 5 6 7 8 9 10 1 Young produced o -0 ) 0-Adult mortality (--. L -... 2J Youngproduced I oc O cT) 1 c.. Oc) c) Adult mortalit C L \\-i I. 1 3 Young produced Ic c1 c) Adult mortality I.
- x.
U_ U-Adult mortality
- -_.1_
_ r H 4 Young produced I141
- i'-
u-Y Youngprodued It fj J C) 1t. 7-iT I sI 1,z., Adult mortality \\_ _ t-1_ 7 Young produced 0 0 0C)) Adult mortality k_ 7 on rdcd L5 1,3 1 1-1 13 1s W r Total young produced 2z 2A 2-t 31 2-1 32.- Final Adult Mortality L...- Note: Adult mortality (L live, D = dead) Concentration: % Mortality: 07.. Mean Offspring/Female: 27, CONC: 100% Intake Survival and Reproduction Data Replicate number
- Day, 1
2 3 4 5 1 6 7 8 9 10 I Young produced 0" 0 0 0 -) C 1 0 1 1 o IL O dl otlt .__k.. L_ k._ 1 1 2 3 4 1 6 7)._ Adult mortality 1-- 1
- l -
I* I-- I -- Adult mortality L 2 Young produced Co)I 1 I~c T Adult mortality J Young produced 4~L~c LA~o c Adult mortality I (-_".I'-_.. -I_-. Young produced 4 S I L W ts 7 Adult mortality I V 5 Young produced I'Ii I g II l-jL 1 6~ cr-Adult mortalit K-- 6 Young produced jo l lc o c i-ll [7 ] Youngprodueed jq
- t.
i8 IT i a I~ S o jq Tg I Total young produced 1 I " 4 S 3 "2j A SI Final Adult Mortality \\. j _-r X-I Note: Adult mortality (L = live, D dead) Concentration: % Mortality: 0 Mean Offspring/Female:
- 35. (a
% Reduction from Control: - IS. r
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Verification of Ceriodaphnia Reproduction Totals Control-1 Replicate number ToW Day 1 2 3 4 5 6 7 8 9 1 Tt0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 3 0 00000 0 0 0 0 4 4 4 5 4 4 4 4 4 4 4 41 5 10 10 9 10 9 9 11 10 13 11 102 6 0 0 0 0 0 0 0 00 0 0 7 15 13 13 16 14 17 15 13 15 15 146 Total 29 27 27 30 27 30 30 27 32 30 289 11.3% Replicate nunrer 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 4 5 4 4 4 5 44 5 10 11 l0 12 10 10
- 11.
12 11 11 108 6 0 0 0 0 0 0 0 0 0 0 0 7 14 14 16 T4 1 18 161 13 5 18 155 Total 28 29 31 31 31 33 31 29 30 34 307 22.6% Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 3 4 5 5 5 4 4 43 5 13 13 10 11 11 10 11 13 12 10 114 6 3 0 0 0 0 0 0 0 0 0 3 7 13 17 17 14 16 16 19 1 19 16 165 Total 33 34 32 28 31 31 35 36 35 30 325 45.2% Day Replicate number Total 1 2 3 _4L 5 6 7 18 19 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 5 4 5 4 4 4 44 5 14 11 11 11 10 12 12 13 10 11 115 6 2 0 0 15 0 0 0 0 16 0 33 7 18 18 17 0 19 16 17 14 0 19 138 Total 38 33 33 31 34 32 34 31 30 34 330 72.6% Replicate number Total Day 1 2 3 4 5 6 7 8 9T 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 5 5 5 5 4 6 47 5 11 14 10 14 11 11 15 10 12 13 121 6 16 0 16 0 0 0 0 0 0 0 32 7 0 19 0 18 18 16 15 18 19 15 138 Total 31 37 31 36 34 32 35 33 35 34 338 100% Re plicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 6
- 5.
5 4 5 5 5 48 6 16 0 18 00 0 0 0 0 0 34 7 15 0 18 18 17 19 14 19 17 137 Total 32 33 33 36 33 36 34 33 38 34 342 Control-2 Replicate number Total Day 1 2,, 3 4 5 6 7 8 9 1 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4
- 4.
5 4 4 3 4 4 40 5 10 9 9 9 11 10 10 11 10 12 t01 6 0 0 0 0 0 0 0 0 0 0 0 7 15 13 17 16 14 15 14 17 13 16 150 Total 29 26 30 29 30 29 28 31 27 32 291 100% Intake Day Replicate number Total 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 5 4 4 4 5 4 44 5 15 11 11 11 10 14 12 13 10 12 119 6 0 1 0 0 0 0 17 0 0 0 17 7 19 15 18 18 16 15 0 19 18 18 156 Total 38 30 34 34 .31 133 1 33 1 36 33134 336
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number: 2878 Reveiwed by: ______ Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (%) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) pooled controls (.) Control - 1 29 27 27 30 27 30 30 27 32 30 100 28.9 6.2 Not applicable 11.3%, 28 29 31 31 31 33 31 29 30 34 100 30.7 6.0 -6.2 22.6% 33 34 32 28 31 31 35 36 35 30 100 32.5 7.8 -12.5 45.2% 38 33 33 31 34 32 34 31 30 34 100 33.0 6.9 -14.2 72.6% 31 37 31 36 34 32 35 33 35 34 100 33.8 6.0 -17.0 100% 32 33 33 36 33 36 34 33 38 34 100 34.2 5.5 -18.3 Control-2 29 26 30 29 30 29 28 31 27 32 100 29.1 6.2 Not applicable 100% Intake 38 30 34 34 31 33 33 36 33 34 100 33.6 6.8 -15.5 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 2.123 7.3 1.586 5.5 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA ( 10th percentile) = 11%. Upper PMSD hound determined by USEPA (90th percentile) = 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 1 t/14/2006 Test ID: CdFRCR Sample ID: TVA/ Sequoyah Nuclear Plant - Outfall 101 End Date: 11/21/2006 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaptnia dubia Comments: Non-treated Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 29.000 27.000 27.000 30.000 27.000 30.000 30.000 27.000 32.000 30.000 11.3 28.000 29.000 31.000 31.000 31.000 33.000 3t.000 29.000 30.000 34.000 22.6 33.000 34.000 32-000 28.000 31.000 31.000 35.000 36.000 35.000 30.000 45.2 38.000 33.000 33.000 31.000 34.000 32.000 34.000 31.000 30.000 34.000 72.6 31.000 37.000 31.000 36.000 34 000 32.000 35.000 33.000 35.000 34.000 100 32.000 33.000 33.000 36.000 33.000 36.000 34.000 33.000 38.000 34.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 28.900 1.0000 28.900 27.000 32.000 6.201 10 32.183 1.0000 11.3 30.700 1.0623 30.700 2&.000 34.000 5.957 10 -1.939 2.287 2.M23 32.183 1.0000 226 32.500 1.1246 32500 28.000 36.000 7.845 10 -3.878 2.287 2123 32.183 1.0000 45.2 33.000 1.1419 33.000 30.000 38.000 6.851 10 -4416 2.287 2 123 32.183 1.0000 72.6 33.800 1.1696 33.800 31.000 37.000 6.047 10 -5.278 2.287 2.123 32.183 1.0000 100 34.200 1.1834 34.200 32.000 38.000 5.479 10 -5.709 2.287 2.123 32.183 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolnogoov D Test indicatm normal distribution (p > 0.01) 0.736118853 1.035 0.267812653 -0.34924831 Bartlett's Test indicates equal variances (p = 0.88) 1.74052 15.08627224 lypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 2122949295 0.073454993 40.85666667 4.309239259 1.613-06 5,54 Treatments vs D-Control Linear Interpolation (200 Reanmples) Point SD 95% CL Skew IC05 >100 ICIO >100 IC15 >100 10 - IC20 >100 0.9 1 IC23 >100 0.80 1C40 >100 0.7 1C50 >100 0.0 ¢ 0.5" 0.4 0 o 0.3 I 0.2 0.1 0.0 0 -0.1 -0.2 -0.3 0 20 40 60 0 100 120 Dose % Dose-Response Plot 40 35 30 . 25 n 20 15* 10. 51 ...............................1-tail, 0.05 level of significance 5: U 6 sqn_lI-]4-O6data
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 14-21, 2006 Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/14/2006 Test ID: CdFRCR Sample ID: TVA/ Sequoyah Nuclear Plant - Intake End Date: 11/21/2006 Lab ID: ETS-Eavir. Testing SoL Sample Type: DMR-Dischlage Monitoring Report Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Coreumeats: Non-treated Conc-%/o 1 2 3 4 5 6 7 8 9 10 D-Control 29.000 26.000 30.000 29.000 30.000 29.000 28.000 31.000 27.000 32.000 100 38.000 30,000 34.000 34.000 31.000 33.000 33.000 36.000 33.000 34.000 Transform: Unvaansformed I-Tailed Isuoanie Conc-% Mean N-Me-n Mmn Min Mal CV% N t-Stat Critical MSD Mean N-Mean D-Control 29.100 1.0000 29.100 26.000 32,000 6.158 10 31.350 1.0000 100 33.600 1.1546 33.600 30.000 38.000 6.758 10 -4.920 1.734 1.586 31.350 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.973339081 0.868 0.197458597 0.234273437 F-Test indicates equal varianres (p = 0.49) 1.605536342 6.541089535 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homosetdastic t Test indicates no significant dtfferences 1.586139066 0,054506497 101.25 4.183333333 1.IE-04 1, 18 Treatments vs D-Contuol Linear Interpolation (200 Resamples) Point SD 95% CL Skew [Cos >100 IC1O >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 08. IC40 >100 0.7, IC50 >100 0.6-0,5. c 0.4-0,3 0.2 0.1 0.0 -0.1 -0.2 -0.3" 0 20 40 60 80 100 120 Dose % Dos e-Response Plot 40 35 30 1-tai, 0.05 level c 25 at -significance -o20 5 6 qsqnl-I 4-O6data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses Project number: 2878 Reviewed by : l-Concentration Parameter Da 0 Day 1 Day 2 Day 3 Dai 4 Day 5 Day 6 Initial Final Initial IFinal Initial IFinal Initial Final Initial Fnl Initial Final Initial Finial pH (SU) 7.67 7.71 7.81 7.48 7.521 7.34 7.55 7.27 7.77 7.51 7.81 7.67 7.81 7.47 DO (mg/L) 8.1 8.3 8.21 7.7 7.81 7.61 7.71 8.21 7.7 7.9 7.81 7.91 8.0 .8.2 Conductivity (pmhos/cm) 314 320 3 316 Control Alkalinity (mgIL CaCO3) 59 57 59 60 Hardness (mg/L CaCO 1) 93 93 91 93 Temperature ('C) 24.7 25.1 24.9 24.6 24.6 25.2 24.8 24.9 24.7 25.1 24.8 24.9 24.6 24.8 PH (SU) 7.65 7.69 7.77 7.46 7.53 7.34 7.38 7.27 7.26 7.51 7.48 7.67 7.76 7.48 11.3% DO (mg/L) 8.0 8.3 8.0 7.7 7.9 7.6 7.9
- 8.0 7.9 7.8 7.9 7.8 8.0 8.2 Conductivity (pmhos/cm) 299 295
__306 289___ 293 291 292 Temperature (oC) 24.7 24.8 24.8 24.8 24.7 24.8 24.9 24.9 24.7 24,8 24.7 24.7 25.1 pH (SU) 7,66 7.67 7.76 7.48 7.54 7.351 7.38 7.27 7.27 7.52 7.48 7.68 7.77 7.50 22.60 DO (mg/L). 8.0 8.3 8.1 7.7 8.0 7.6 8.0 7.8 7.9 7.7 7.9 7.8 8.1 8.3 Conductivity (Mtmhos/cm) 285 L86 296 273 278 278 278 Temperature (°C) 24.7 24.8 24.8 24.6 24.7 24.7 24.9 24.8 24.9 25.3 24.9 25.1 24.7 25.0 pH (SU) 7.66 7.67 7.74 7.48 7.53 7.35 7.36 7.28 7.26 7.51 7.47 7.68 7.75 7.50 45.2% DO (mg/L) 8.0 8.3 8.1 7.8 8.1 7.6 8.0 7.7 7.9 7.8 8.0 8.0 8.1 8.3 Conductivity (iimhos/cm).257 258 265 246__ 251 252 250( Temperature (OC) 24.8 24.9 24.8 24.9 24.7 25.1 24.9 24.9 25.0 25.0 24.9 25.2 24.8 25.2 pH (SU) 7.65 7.66 7.731 7.47 7.52 7.38 7.34 7.31 7.24 7.50 7.45 7.69 7.73 7.50 72.6% DO (mg/L) 8.1 8.3 8.11 7.8 8.0 7.6 8.0 7.8 8.0 7.9 8.1 7.9 8.2 8.2 Conductivity (Imhos/cm) 222 223 231 211 217 213 217 Temperature (C) 24.8 24.8 24.8 24.8 24.8 25.11 25.0 24.9 25.2 25.0 25.0 25.1 24.8 25.2 niiH (SU) 7.64 7.67 7.71 7.47 7.51 7.39 7.331 7.301 7.22 7.50 7.431 7.701 7.70 7.54 DO (mgIL) 8.0, 8.3, 8.2 7.8 7.91 7.71 8.21 7.81 8.21 7..9 8.21 7.91 8.3 8.1 Conductivity (plmhos/cm) 188 186 198M 17 18 884 100% Alkalinity (mg/L CaCO 3) 66 65 Hardness (mg/L CaCO3) 77 79 Total Residual Chlorine (mg/L) <0.10 010 Temperature (°C) 25.2 25.0 24.7 24.8 24.91 24.9 25.2 24.7 25.3 25.1 25.0 24.7 24.8 25.2 pi1 (SU) 7,65 7.671 7.71 7.48 7.51 736 7.301 7.291 7.201 7.471 7.41 7.70 7.691 7.52 DO (mg/L) 8.0 8.31 8.3 7.7 7.8 7.9 8.3 7.8 8.2 8.0 8.2 8.1 8.4 8.3 Conductivity (pmhos/em) 18V8 198 18 182 19 8 100% Intake Alkalinity (mg/L CaCO 3) 64 66 Hardness (mg/L CaCO3) 79 7 77 Total Residual Chlorine (mg/L) <0.10 <0.10 Temperature ('C) 25.0 24.81 24.8 24.9 24.8 24.91 25.1 24.9 24.9 25.1 24.9 24.9 24.8 25.1
Environmental Testing Solutions, Inc. Page 6 of 7 Environmental Testing Solutions, Inc. Page 6 of 7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date: tI \\ -,q0 Daily Chemistry: Day 0 "[12 Concentration Parameter CONTROL pH (S.U.) 61 .2. DO(mgfL) 8.1 6.2 Conductivity Alkalinity (mg CaCO3/L) A Hardness (mg CaCO3/L-) Temperature (°C) Iq.--I t-L%*' -- q Il"** Q-0I z. pH (S.U.)
- q.
q 9 q,53 143,4 11.3% DO (mg/L) 0 Conductivity (ymhoslcm) Temperature (°C) -V44, -L "A.k A. -I ILA. k pH (S.U.) 9.-e* L ".(,-4 3-Ba3 "(a 9,5L ý4. 59 22.6% DO (mg/L) 0,0 to Conductivity pH (s.u.) 2*.,o~ q.. L. Lfq ")6 9.35 472.% DO (mg/L)
- .1 I**
.6 "9l .0 1G (pmhos/cm) Temperature (°C) -LA. 9.
- 1. -
7A 702o% DO (mg/L) (30 1 2-9.. I-O Conductivity (ymrhos/cmr) I31 I = 0550(0 Temperature (C ) A. aP -I 12.% DO(mg/L) (3'3 ConductivityAP (ymhos/crn) A O TR chlorine 01 .0 (rag/L) Temperature(0 C) (S-1 .a' 21 T -9 t2 & f I.- pH (S.U.) "91,04 4 q,51 100% Intake DO (mg/L) 6o0 "*q Conductivity 18Ae (pmhos/cr)i Alkalinity (mg CaCO3/L) Hardness (mg CaCO 3 L) TR chlorine (mg/L) Temperature ('C), s I.o ,o 77-4-.l 24 e z, - 1 q.9 Initial Final I Initial I Final Initial I Final I
Environmental Testin Solutions IDC. Page 7 of 7 Enirnpnd s tin ~~tnS Inc ae7o Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: 1L( -oU Day 3 I4 I5 !6 i* Concen-Parameter trationW CONTROL _pH (S.U.) "41 1 ___(__ 1 __3____ Conductivity Pt (mg CaCO3/L) Hardness (mg CaCO 3/L) A" '8 Temperature ('C) li -LA 1c 71 1.I\\Z4-A. pH (S.U.) I Q __(- 1 1_._ 4 3_q 11.3% DO(mg/L)
- 6.
qDq '4. 8.0 Conductivity (iimhos/cn) 1 Temperature (°C) ".0 .1 "AA.'7 "/. "A. 2.4 "-¶.\\ pH (S.U.)
- 9.
Z 1 -Z 22.6% DO (mg/L) 8.*3 e-o1"' d £. Conductivity (ýimhos/cm). Temperature (C) VLIq l'4- "t.-S._ "A.. pH (Su.) 5D 3 .q q _o3 45.2% DO (mg/L) 0,09.- -,C .1 f...3 Conductivity (pmhos/cm)
- f Temperature (C)
.'4 -ts", -D2Q. Z9. kzn, PH (S.U.) 2,3 q3 0 4 ~g _777 1.3 4,0 ca"* C?.q 72.6% DO (mg/L) 0 Conductivity (limhos/cm) _____Temperature (C) Zq~.L3 SZ ~.0 2.~Z.I 1.{ <f.L Alkalinity TR Chlorine (m)*** 1 Temperature (C) IS. I I Z I z*3 n.0 2.LI PH (S.U.) 2-0 9.9?.ug 100% DO(mg/L) ,.I -.z 6.1 ,L £ 3 Intake (Trnhos/cm) ) 2*.
- 4.
7 Alkalinity Hardness 1*~* 11 I(rag CaCOFL) ITR chlorine (mg/L) ?q** I OJ 7Zq. I FTem-perature (0C) -1 It qq I:,
- lI*t*!
i*!*~ -s Initial Finai Initial Final I Initial 1 Final I Initial Final I
Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales prormelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project#: Ztik County:. Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 11.3 22.6 45.2 72.6 100 Each concentration was treated Effluent volume (mL) 282.5 1130 1815 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2217.5 1370 685
- 0.
to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information:
- .2-Test information:
Organism age: -A S a**, L3t> Randomizing template: &.ue... Date and times organisms It-tb-o0 iM-o -t O ClO Incubator number: were born between: Organism source: NV6% ePsr, (k, Q? .o-,O. Artemia lot number: 1 10zo4 U Transfer bowl information: pH = Temperature = °C Total drying time: 2,-25 ý v46o '.1 Date/Time in: Il-1.-Oka Io Average transfer volume: Date / Time out: -a'a,- 00 Oven temperature: (,0 '0, Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 to______ 1(.0O l'-o( it2OlA I's.(,)I CA 4 1-tI -01.& 10001 l160-1'42) -9I -t j Ot 0(P ('I " t I 3.o1'kt1 5 11-101-06o I0AI I ko 00 t -i I "/.- l -c2.-08
- w.
111 -1tV. C) G O-L ID* 2 IIt.-t. iobtOO 1 q7_ \\-*-1.-O*o 0 0 Xb\\\\%, Vb A 6 11,20-06a O0CISS loo0-._ of- %kI 0td. 60tC"r1 7 Control information: Acceptance criteria Summary of test endpoints: % Mortality: 20% 7-day LC5 0 > I 007o Average weight per initial larvae: I.iS I NOEC -00 Average weight per surviving larvae: O.-I's > 0.25 mg/larvae LOEC "> I O7. CLIV )7 IC 2s
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimeph ales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: W\\--Lk-u6 Survival and Growth Data Day CONTROL 11.3% 22.6% A B C D E F G H I J K L 0 10 /1 0 0 /0 /0 10 /0 AD /0 t1 /0 1 ' C /t0 10 1 t /0 (0 to to / 1`0 to 20 t 0 (o 0 ( /0 (0 (0 0 /0 (0 /0 I0
- 15) 1 10
/ C) IO D O I ( IO 1c) .1©0 to 1 6/0 /0 /O /0 /0 10 10 C0 t0 /0 7 /0 I0/0 /0 0 /b /O 10 10 '0 PS A = Pan weight (mg) Color identification: 6-%"-1 13.91 13.3b I5.3H (5.0q 13 lq.q/o 13.a,, 13.(o* 15.38 14.35 )5,;a 13.rq Analyst: Ll ri* B = Pan + Larvae weight (mg) Analyst: 1200X.'73 1313 aa.R aIt aa5O X.-H3 a115 a6 &3.7 B3,A4, -(.16 Larvae weight (mg) = A - B g.o 1 ' s ' "1 ' e q t 7.2 k -I*7 ' t q 3 ° , ° Weight per initial number of larvae (mg) = C / Initial number of larvae ON 4 0* "0 t" 1a, tD -'C', 0" 0-Average Percent weight per reduction initial from control 0 1 [number of (0) larvae (mg)L o.- Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: 4 r Comments:
,..ntai Testing Solutions, Inc. Page 3 of 6 LII,,I! Ull Species: Pimephalespromelas Client: TVA Sequoyah Nuclear Plant - UV-treated Date: I\\- 0c,, Survival and Growth Data Day 45.2% 72.6% 100% M N 0 P Q R S T U V W X 0 /0 /0 /0 /0 /0 /0 /0 /0 io 1o / 0 1 / t (0 /0 10 10 /0 /0 /0 /l /0 /0 /0 2 I t ( I 10 /t oO /0 (0 /0 C0 /0 /0 /0 3/ t1 I 0 10 /0 10 t1 rj /1 0 0 t( 4/0 /0 I0 / /C /0 1C /I C'O / 1 /0 ID 5 It O /0 / 10 10 1D 10 10 10 10 C 0 tO 6 I0 I/) (O to /0 /0 /t io (0 /0 /0 /0 H/ )0 (0 10 ! / o /0 0O /0 to (0 A Pan weight (mg) Color identification: SiLLjp_'5¢ -*+ 1 a 1 3.1o9 I435 1453 1H.9r9 Nm-I 15.0: Analyst: (J,.: f:_: B = Pan + Larvae weight Analyst: A a(.o8 a3.5% i 0 3,oari ai.Qr7
- .a*.i ao.f, a.qi Larvae weight (mg) = A - B 6,IC 9.14
-7. 1Z I'. S6' 1. 9 1 1.Tt, 1L S 7-7 5 4 o 05 -,3 "l -7. 10 -7:12. W eight per initial number 'IVA V of larvae (mg) ( \\0 A, A = C / Initial number of larvae \\O
- A Average Percent weight per reduction initial from control 03 1
.\\ 0.-. number of (%) larvae (mg) Comment codes: c = clear, d dead, fg fungus, k = killed, m = missing, sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w wounded. Calculations and data reviewed: Comments:
Environmental Testing Solutions, Inc. Page 4 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: i.,L 1*-ok-0 Survival and Growth Data Day 100% Intake Y Z I AA BB 0 O 10 10 IC) /D 10 tC /0 2 /0 10 (0 ('0 /0 1 t) (0 '0 ______4 /C ) /C) /C) 40 /O 0 /0 /0 6( tO /0 10 60 (C CO t0 A = Pan weight (mg) Color identification: Analyst: (.- 4" B = Pan + Larvae weight (mg) at,:7 io caýa., ýt.s) aaxc~b Analyst: 09P* Larvae weight (mg) = A - B Weight per initial number of larvae (mg) A U A,\\ -*~- C / Initial number of larvae 'A to o" o" o Average Percent weight per reduction initial from control
- o. '7 6.
number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg, fungus, k = killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwved by: Project number: 2878 Not for Compliance Assessment, Internal Laboratory QC Concentration (%l Replicate initiul nonbr of Pinot number ut tarvae A - Pan wright B = Pan + L.-re L..,e wright (rg) Weight I Survliing Men weight Coeficient of variation Weight / luitial useeber Mean, sutivt Mean weight / Co tt6cien' of Percent reduction from 2l-cvae (mg) weight (mog) - A - B bomber of tnb, n (.og) Surviving nueber'of (-t. r ~tht.,,urwlht oflorvue (tag) (%) Initial number of variation (pt*-,u "re control (%) " ev-e (tog) n - f b -,t ) (% ) la-re (tog) ,..IIlvun h., tt.n. A 10 10 13.91 22.00 8.09 0.809 0.809 Control B to10 10 13.38 20.73 7.35 0.735 0.775 4.1 6.735 100.0 0.775 4.1 Not applicable C 10 10 15.34 23.23 7.89 0.789 0.789 D 10 to [S.07 22.73 7.66 0.766 0.766 E 10 9 13.02 21.18 8.16 0.907 0.816 1h3% F 10 10 14.70 22.50 7.80 0.780 0809 9.7 0.78097.5 0.787 6.1 -1.5 G o0 10 13.22 20.43 7.21 0.721 0.721 H 10 10 13.66 21.95 8.29 0.829 0.829 1 10 9 15.3B 22.64 7,26 0.807 0.726 22.6% J t0 10 14.35 23.74 9,39 0.939 0.822 10.2 0.939 97.5 0.802 12.2 -3.5 K 10 to 15.20 23.24 8.04 0.804 0.804 L 10 10 13.77 21.16 7,39 0.739 0.739 1 1 M 10 10 14.58 20.68 6.10 0.610 0.610 45.2Y., N 10 10 15.44 23.59 8.14 0.814 0.743 .14.0 400.0 0.743 14.0 4.1 0 10 10 15.14 22.26 7,12 0.712 0.712 P 10 10 14.29 22.65 8.36 0.836 0.836 Q 10 9 15.57 23.44 7.87 0.874 0.787 72.6% R. 10 10 13.64 21.40 7.76 0.776 0.792 7.1 0.776 97.5 0.770 1.9 0.6 S 10 10 14.35 21.97 7.62 0.762 0.762 T 10 10 14.53 22.07 7.54 0.754 "0.754 U 10 10 14.97 22.02 7.05 0.705 0.705 100% V 10 10 14.74 22.11 7.37 0.737 0.731 4.2 0.7371.0 0.731 4.2 5.6 W 10 10 13.72 20.82 7.10 0.710 0.710 X 10 t0 15.02 22.74 7.72 0.772 0.772 Y 10 10 14.40 20.86 6.46 0.646 0.646 100% Intake Z 10 10 14.60 22.28 7.68 0.768 0.726 8.1 0.768 100.0 0.726 8.1 6.3 AA 10 10 14.64 21.81 7.17 0.717 0.717 BB 10 10 14.34 22.06 7.72 0.772 0.772 Outfall 101: Donnell's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: MSD = Minimum Significant Difference 0.1095 14.1 0.0649 8.4 PMSD = Percent Minimum Significant Diffetence PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.0% from the conorol (deteissined through reference toxicant testing), Lower PMSD bound detetstoined by USEPA (1061 percentile) ý 9.4%. Upper PMSD bound deterimined by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales giowth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 14-21, 2006 Statistical Analyses Larvl Fish Growth and Survival Test-7 Day Growth Start Date: 11/14/2006 Test ID: PpFRCR. Sample ID: TVA] Sequoyah Nuclear Plant - Outfalt 101 End Date: t1/2112006 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCHIR-EPA-821-R-02-0t3 Test Species: PP-Pirnephales promelas Comments: UV-treated Cone-% 1 2 3 4 D-Control 0.8090 07350 0,7890 0.7660 11.3 0.8160 0,7800 0.7210 0.8290 22.6 0,7260 09390 0.8040 0.7390 452 0.6100 0.8140 0.7120 0.8360 72.6 0,7870 0.7760 0.7620 0.7540 100 0.7050 0.7370 0.7100 0.7720 Transform: Untrans forted I-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7748. 1.0000 0.7748 0.7350 0.8090 4104 4 0.7878 1.0000 11.3 0.7865 1.0152 0.7865 0.7210 0.8290 6.146 4 -0.259 2-410 0.1095 0.7878 1.0000 22.6 0.8020 1.0352 0.8020 0.7260 0.9390 12.157 4 -0.600 2.410 .0.1095 0.7878 1.0000 45.2 0.7430 0.9590 0.7430 0.6100 0.8360 13.974 4 0.699 2.410 0.1095 0.7564 0.9602 72.6 0.7698 0.9935 0.7698 0.7540 0.7870 1.905 4 0.110 2.4t0 0.1095 0.7564 0.9602 100 0.7310 0.9435 0.7310 0.7050 0.7720 4.205 4 0.963 2,410 0.1095 0.7310 0.9280 Auxiliary Tes ts Statistic Critical Skei Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.979922116 0.884 0.130565288 1.129624347 Bartlett's Test indicates equal variances (p = 0.03) 12-26457691 15.08627224 Hypothesis Test (0-tail, 0.05) NOEC LOEC ChV TU MSDn MSDp MSB MSE F-Prob dlf Duenetts Test 100 >100 1 0,109544232 0.141393007 0.002832567 0.004132139 0.640509248 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples) Point SD 95% CL(Exp) Skew IC05 81.252 IClO >100 ICt5 >100 1.0 IC20 >100 0.0 IC25 >100 1C40 >100 0.8
- tC50
>100 0.7 0.0. =o~s 0.4 0.2 0.1 0.0 -0 1....... 1 0 20 40 60 30 100 120 Dose % Dose-Response Plot 0.8 7 .i 1-tail. 0.05 level 0of significance o0.5 10 0.4" 0.3 0.2 0.1 0 o sqtl -i-0-O6data
TVA / Sequoyah Nuclear Plant, Intake UV-treated November 14-21, 2006 Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date. 11/14/2006 Test ID: PpFRCR Sample ID: TVA / Sequoyah Nsclear Plant - Intake End Date: 11/21/2006 Lab ID: ETS-EnvT. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: FWCIIR-EPA-821-R-02-013 Test Species: PP-Pimephales piomelas Comtmnents: UV-treated Conc-Va 1 2 3 4 D-Control 0.8090 0.7350 0.7890 0.7660 100 06460 0.7680 0.7170 0.7720 Transform: Untransfornied 1-Tailed Isotonic Con.-% Mean N-Mean Mean Min Max CV% N I-Stat Critical MSD Mean N-Mean D-Conted 0.7748 1.0000 0.7748 0.7350 0.8090 4.104 4 0.7748 1.0000 100 0.7258 0.9368 0.7259 0.6460 07720 8.097 4 1,467 1.943 0.0649 0.7258 0.9368 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.919678569 0.749 -0.80552162 -0.01712049 F-Test indicates equal variances (p = 0.34) 3 416288853 47.46722794 Itypothesis Test (1-tail, 0.05) MSDu MSDp MSBH MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.064918656 0.083793038 0.004802 0.00223225 0.192823291 1, 6 Treatments vs D-Control Linear Interpolation (200 Resamiples) Point SD 95% CL(Exp) Scew IC05" 79.056 ICtO >100 IC15 >100 1.0 IC20 >100 0.90 IC25 >100 IC40 >100 0.8-1C50 >100 07 - indicates IC estimate less than the lowest concentration n0.8-0.5 0.4 0.3 0.2 0.1 0.0 0 20 40 60 80 100 120 Dose % Dos e-Response Plot 0.9 0.8 14ail, 0.05 level 0.a e significance z 0.6 2 0.5 to 01 0.3 0.2 0.1 0 05 sqn_I]-]4-O6data
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 14-21, 2006 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Daily Chemical Analyses Project number: 2878 Reviewed by: Concentration Parameter Da 0 Da I Da 2 Day 3 Day 4 Day 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.68 7.59 7.69 7.48 7.57 7.20 7.49 7.29 7.50 7.27 7.61 7.46 7.83 7.11 Control DO (mg/L) 8.1 7.8 8.2 7.7 7.8 7.3 7.8 7.6 7.9 7.1 7.9 7.5 7.8 7.4 Conductivity (0mhos/cm) 307 308 307 296 301 297 300 Temperature (OC) 24.7 24.5 24.6 24.4 24.8 24.6 24.8 24.6 24.8 24.3 24.9 24.5 24.7 24.3 PH (SU) 7.68 7.53 7.68 7.45 7.57 7.17 7.48 7.29 7.50 7.27 7.63 7.47 7.83 7.11 11.3% DO (mg/L) 8.1 7.8 8.2 7.7 7.9 7.2 7.8 7.5 7.8 7.0 7.9 7T5 7.8 7.4 Conductivity (Ilmhos/cm) 301 302 304 288 299 298 295 Temperature (oC) 24.7 24.2 24.7 24.2 24.8 24.8 24.9 24.7 24.9 24.6 24.9 24.1 24.9 24.7 pH (SU) 7.68 7.55 7.68 7.48 7.58 7.18 7.47 7.26 7.50 7.26 7.64 7.43 7.82 7.10 DO (mg/L) 8.1 7.7 .8.2 7.7 8.0 7.2 7.9 7.6 7.8 6.8 7.8 7.4 7.9 7.4 Conductivity ietmhos/cm) 281 285 293 276 285 282 279 Temperature ( °C) 24.8 24.7 24.7 24.2 24.8 24.7 24.9 24.7 24.9 24.7 24.9 24.2 24.9 24.6 PH (SU) 7.69 7.55 7.68 7.46 7.58 7.13 7.44 7.26 7.49 7.26 7.64 7.43 7.79 7.11 DO (m a/L) 8 78 8.2 7.7 8.1 6.9 7.9 7.6 7.9 6.8 7.9 7.3 8.0 7.5 45.2% Conductivity tmhos/cm) 258 259 267 248 258 254 256 Temperature ( °C) 24.8 24.3 24.9 24.3 24.8 24.5 24.8 24.9 24.5 24.9 24.2 24.9 24.6 pH (SU) 7.69 7.55 7-67 7.40 7.57 7.11 7.43 7.25 7.48 7.26 7.59 7.50 7.77 7.21 ,DO (mg/L) 8.2 7.8 8.1 7.5 8.1 6.8 7.9 7.6 8.0 7.0 8.1 7.4 8.0 7.5 'Conductivity (punhos/cm) 218 222 230 216 225 220 219 Temperature (OC) 24.9 24.3 249 24.3 25.0 24.5 24.9 24.5 25.0 24.5 25.0 24.2 24.9 24.3 PH (SU) 7.69 7.67 7.43 7.57 7.13 7.39 7.36 7.45 7.26 7.58 7.43 7.74 7.16 DO (mg/L) 8.1 7.9 8.1 7.4 7.9 6.8 8.0 7,5 8.0 68 8.1 7.4 8.0 7.6 Conductivity (pmhos/cm) 188 192 202 188 189 188 190 Temperature (C) 25.1 24.3 24.9 24.2 25.0 24.6 24.9 24.7 25.1 246 25.0 24.5 25.0 24.5 pH (SU) 7.68 7.58 7.68 7.43 7.55 7.16 7.36 7.48 7.29 7.25 7.57 7.36 7.72 7.07 100% Intake DO (mg/L) 8.2 7.9 8.2 7.4 8.1 6.9 8.0 7.4 8.1 69 82 7.1 8.0 7.4 Conductivity (pimhos/cm) 186 185 195 190 192 184 189 Temperature (OC) 25.0 24.4 24.8 24.2 24.9 24.4 24.9 24.8 25.0 24.6 25.0 24.3 24.8 24.2
Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: I k-14-O60 Daily Chemistry: Day 0 1 2 Analyst Concentration Parameter Z CONTROL pH (S.U.)
- q.
,(q 6-1 q,.7 9 ` 4 DO (mg/L) a.2, Conductivity (1imhos/cm) Temperature ('C) 2?.14 . S T14- -. pH (S.U.) "4.W 6 2 -53 ý.(o e3 q:. Lis-r, -- ".9 11.3% DO (mg/L) .1 6 53-'2 Conductivity (iimhos/cm) Temperature (°CQ 14~.1 114."- 7- ~ q -ILq .'7. ".q - -"q..j pH (S.U.) q'La 6 11.5 (e 22.6% DO (mg/L) 63-D Conductivity ([imhos/cm) 2645 Aq3 Temperature (°C) LA.,-
- 14. -1 2.'A 2t.2 -
"4.. pH (S.U.) "4.toq "3.S .(o6 1.410 1 .'51 9 43 45.2% DO (mg/L) Conductivity (ýtmhos/cm) ASq Temperature (°C)
- 14.
2 pH (S.U.) 3-C. (O5 "4,-, 0 72.6% C v DO (mg/L) 6,2 Conductivity ~ (jimhos/cm) Temperature (°C) t.. 0 pH (S.U.) ". tq 4 .14:3 100% DO (mgf/L) O.1 I Conductivityi6 (pmhos/cm) Temperature (°C) "c.S 2.4-,3 -z"2IA 24l. .o -. 0 .6 PH (S.U.) q* t .!5 Q '. ( 0 6 . ' "+.Y) .? 6 - 1/ 100% Intake DO (mg/L)
- 2.
Conductivity 'ss (Ttmhos/cm) ______Temperature(C S2.-1 7I qL 7q Z4 Initial Final Initial I Final I Initial Final
Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimnephales promelas Client: TVA/ Sequoyah Nuclear Plant-UV-treated Date: 11-14-o0 Day 4 5 6 Analystat i o___ 1'k* Concen-Parameter tration CONTROL pH (S.U.) I4 2 ,.'50 q4 (t'P DO (mg/L) Conductivity (_*mhos/cm) Rl 30 Temperature (°C) Z4.A "t 1.s -t. 1A 1" -tqC 7-4.1) pH (S.U.) 9.-2q --4.b ý. q. 11.3% DO (rg/L) Conductivity (jýimhos/crn) Temperature(0 C) (.Q "4.1 A .qz L 2q. 9 "q-pH (S.U.) "4- .o L44 q.q3
- 42. 10 22.6%
DO (mg/L) -t Conductivity (pimhos/cm) Temperature (0C) '134
- 14.
7.-4.9 "2.Z Z.q.2.4A, pH (S.U.) 72( -- 49 qz ").'4l 72.6% DO (mg/L) ":). O 0.9. ,O Conductivity (ýmhos/cm) Temperature(0 C) (lQ.q O 1qs "z"O .9 1 -L q,9 q 6 7.% pH (S.U. 1% DO (mgL) ( Conductivity e*s (.umhos/cm) P A 57 Temperature (0C) 2... z.o 7q.__ ".q.S pH (S.U.) ._ A i6_ -h9.43-. 100% DO (mg/L) 6.( 8-0 Intake 0, )("A "0f (- Conductivity (pmhos/cm) 106 1197_ (CIO Temperature(°C '1S.O ".W 2(.O Z4.3 .Z'1. ) V4.LS Initial I Final I Initial (( Final I Initial Final [ Initial 11 Final
Environmental Testing Solutions, Inc. Page 1 of Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO/L I-r 4 AnalystI
- -*r*g-
']'- Date analyzed s -t 1 Titrate samples to pH = 4.50 S.U. Titrant normalit and multiplier determination: pH of Normality Normality (AV) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na2 CO3 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E N x 500 4.5 S.U. number number (E) (acceptable range = 0.018 - 0.022) lboratory control standarOk Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable range (mg CaCO3IL) (ml) ml ml ml = 90 to 110%) 1Ia 3 100 1oo i*.a '.b CI.- / Duplicate sam ple precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (Mg CaCO3/L) ((S - D) /[(S+D)/2]} x 100 number (ml) ml ml ml (acceptable range = +/- 10%) If,5Is 0 S'j l-l6 too -3.2-10,3 s L, Duplicate .O 3.2-D 33 Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mag CaCO3IL) (mg CaCO3/L) (ml) ml ml ml I~A)I* 00 aL~5 36b e-j jo. Sample alkalinity (B) Measured spike value (MV) % R = MV/ SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO 3/L) 75 to 125%) 33 b Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (rg CaCO3 L) 0Z.OJ~ rnjs5 lzo o 3a9 3&(o 6.Is ___.__ IIOtAo o 0.0 5o,,.e' OI-I.O llo.b3~ 11.0 q 1 2 1 00 -1otq.k _O*__14O_1o ,go.3 3.-A A2 wo llZ.R -. 3. q a q, q z Reviewed by: Date reviewed: DII-l-o
Environmental Testing Solutions, Inc. rage ,..L..- Page f Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst ',,d.j Date analyzed I .6 -c¢ Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality (Nv) of H 2S0 4 pH Factor or Multiplier Deionized Titrant check Begin n= (5 ml Na-CO3 x 0.05)/E = (Nx 50000)/ 100 ml sample water reference standard ml ml ml =Nx 500 4 iL nme ubr E (acceptable range0.8 4.5 S.U. Inumber number (E) (.7-p-.--I P ge l-Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOjIL) (acceptable range (rag CaCOL) (ml) ml ml ml 90 to 110%) P ( / 100 0l0 3mi.3 m41. q.3 10-3 9O ,.%) Duplicate sam ple precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO2 /L) ((S -D) /[(S+D)/2]) x 100 number (ml) ml ml ml (acceptable range +/-10%) o, 11 1.o4 -0 Z .b* )iw Z loo .o 3.5 10.3 s 3S. I Duplicate I 3.5 q" -" 1 D _Coo Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Mrg CaCO03L) 0,,, \\t4 Vk%..o 0 -_a., 1-.9 I-4".3 ti.q 10-3 6 0 -k.5 It -I.T3 A.O 4.3 q o~t Fbt.< "0
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Environmental Testing Solutions. Inc. Page J of Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed (l1.0b Titrate samples to pH = 4.50 S.U. Titrant normaliry and multiplier determination: Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized "-ra. End Total =(5 ml Na 2CO3 x 0.05)/E (Nx 50000)/ 100 ml sample water reference standard ml ml -MI 5/E =IN x 500 = 4.5 S.U. number number (E) (acceptable range = 0,018 - 0.ý Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml = 90 to 110%) I (tQ Z3 100 100 AR-3 3.;0 O. 10 I'3 16 6,61 Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOJL) (mg CaCO /L) (ml) MI. ml MI Roo d:5-5S.Z J T-9. () 10-3 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO 3/L) MV= A - B (acceptable range (mg CaCO3/L) = 75 to 125%T 1o 00 IOo,oIl Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (Mrg CaCO3/L) ok, wl 0.CA t.Tia g L5 43.0 q,5 (.) /6.3 )__0 'e-i\\'o. \\Z-AD 6.c) o 3.J 150 oyi\\\\lq-2. jj ___,Z 3.5 3.o
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- e1o7Z3, I*.q o.2 Reviewed by:
I re Date reviewed: I I ý -I ý -ý6
Environmental Testing Solutions, Inc. ,age o ) Page - Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mrg CaCO3 /L Analyst ") ý Date analyzed /'* E-_0 Titrate samples to pH = 4.50 S.U. T mtai and multiplier determination: pH of Normality (iv) of H 2SO 4 pH Factor or Multiplier Deionized Titrant check Begin I---.4 = (5 ml Na 2CO, X 0.05)/E = (Nx 50000)/ 100 ml sample water reference standard ml ml ml .25, =Nx 500 4.5 S.U. number number (E) (acceptable range "n"-. Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml 90 to 110%) I " ¢,. 100 100 00 c.o'/- Duplicate sample precision: Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /[(S+D)/21) x 100 number " (ml) ml ml ml (acceptable range = 10%) Wi1So,,, 10,-0 31.3 10.1 +0 O.3 s Duplicate D Matrix spike recove r: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mag CaCO3/L) (mg CaCOj/L) (ml) ml ml ml _,_0__-_ (X) 0.o N.S 1L.' 10-3 5D Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) 75 to 125%) I 00
- -)
)00, o/. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L) I-I(,O r-S__ I00 q'5 1A3.3 qCI 10.3 q'- I',lo.*t3,* ~~a.q "-3 (o0 qJ,,0 J ,-0O
- 33. Reviewed by: w Date reviewed:
I - I. 0o
Page _ of Z-Fnvironmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst ,/*Y77 Time initiated:. 1+0[ Date analyzed i- -O(o Time completed: Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E (Nx 50000)/ 50 ml sample number number (E) (acceptable range = 0.018 - 0.022) Nx 1000 I I9 PkOq 0.0 q.C -q0 .o1oz 2-,2 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO3/L) (ml) ml ml ml = 90 to 110%) I ** a,a40 50 1ý1.07 I, 11-1.1 9 0o z 2-3 6° 0. Duplicate sam ple precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range +/-L 10%) Duplicate(B) P4.0 ku.i A ._,l. Matrix spike recovery: Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL) (mg CaCO3/L) (ml) ml ml ml SNs~q
- qL40 bb 1q.O 16A 4 t Ao.z 63 Sample hardness (B)
Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3fL) MV = A - B (acceptable range (mg CaCOj3L) 75 to 125%) L2 Lfl 102,51. Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (mj) ml ml ml Multiplier (mg CaCO3JL) Blank (should be 0 mg CaCO3/L) O.o0 0.,0 ,O' AO0. 2-IND m -ioA [1Its uz I e-I AA-yp H 'b1 Is.D*-(Yo I aa.i* C O-P1 3'z. j4 q.- 111os-oS*tSc A zO _)J q3.2. A-1 -IZ O611%0.0 1 __7-_._ qf -*q 6.-- 'to,,, lkik 1 -3 q.,C) 11-4 1 A.* V 6 Z Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed [ I\\ I-O*
Page age _. _1 P of-O Environmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix; Water, MDL = 1.0 mg CaCO3!L Analyst t Time initiated: Date analyzed 4 Time completed:.. Ž
- LJ
- Titrant normality andi multiplier determination:
Titrant ' Normality criecm-- Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml =.2/E = (Nx 50000)/50 ml sample number number (E) (acceptable range = 1000 Laboratory control standard: Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO 3/L) (acceptable range (mg CaCO3/L) (ml) mI mI mI = 90 to 110%) I,SS q2*o 40 50 'q-q
- q.
a2-I0. qZ1'./ Duplicate sample precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO3JL) {(S - D) /[(S+D)/21)x 100 number (ml) ml ml ml (acceptable range =* 10%) Duplicate (B) i' sD 3 0 Matrbc spike recovery: Reference standard Spike value Sample Spike hardness (A) number . (SV) volume Begin End Total Multiplier (mg CaCO 3/L) (mg CaCO3 L) (ml) ml ml ml Z
- s.
a 1, qo Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (mg CaCO3IL) MV = A - B (acceptable range (Mg CaCOj3L) = 75 to 125%) Wo 3 c) 01 T 'S -I. Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (m. ) ml ml ml Multiplier (mg CaCO 3/L) Blank-(should be = 0 mg CaCO3/L) o0.flIo6 O
- F,/-WO6 Z..
J"5 1(,.2 I.3 ,3' c,.iiis.ol T*JA,,5tj Iol a I.3 -L. 3-0 o,,,l-*.'2-a5T a". Z 3.1 /q/ cp 55.o~ 2 3A .jJ 9 ala it 2-55.b
- 5ve 3.6
-41
- o.
.o 3 ,/.. q0o0 3. Note: If >I5mI oftitrant is used, sample must be diluted. Reviewed by: I C\\\\ I Date reviewed I I I-I Cko (-
Environmental Testing Solutions, Inc. Page A,1 Page I of_ Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 rng!L Meter: Accumet Model AR25 pH/ion Meter Analyst L I77 Date analyzed Iodide reagent: T z a Acid reagent:
- p'q-ý aqr Calibration:
0.1 mg/L I 1.00 mg/L Reference standard number ENSS 3;3
- rN --,,
s'*3 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (m g/L) (rag/L). (acceptable range = 90 to 110%) 0.50
- o. Lo 3
Cl.*o D Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = [(S - D) /[(S+D)/2]} x 100 number (mg/L) (acceptable range = 10%) .4, Duplicate LAG___________._____,_ Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg /L) ~~~ ~Blank (should be= <0.10 mg/L) _________________(.o7c q, 0-LA 'So 5I'hN o40Ud '-'4 0Q 0.3E1
- 0(Ifq.b6 CaLf-e& - SE p
51 lt---t Cloud(potv+/-0-esd VO.otyliy Me,{II0 -5mU-h &AUtkU Wc ui7' C(CeY Na~oye O(~1ILI.Z c 5 i4~~ A O.cOaA c IlI'u _~_ o______-_r__pec , Njo color (o.ooooMTy 0*Iilli b3 PC05 1h W -0P,.,1 O.J QQ d c3y-No OkorO; Ota~ilL1.0d. d OO cteOd'1N (oo Caor-i-e-floo bb M113,0o TVA-5(;# (0 C eov 1,N1,ocO( OY /-..o00037q 15all,b 2-J/ Jkr-C kn, Y11*30 () mtr 1-O0 0 an Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/IL) (acceptable range = 90 to 110%) 0.50o O. q S. (,oZO Reviewed by I IZ I .Date reviewed
- 1. D*
Environmental Testing Solutions, Inc. -q .I9-1/ý Page 30 Page 1 of Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL =0.10 ng/L Meter: Accumet Model AR25 pH/Ion Meter Analyst ( t Date analyzed ll-t(Q-0(D Iodide reagent: 1.- *-77777 Acid reagent: e Calibration: >:.*ý,;:Ž7: a;<:,::I>:" 0.10 mg/L 1.00 mg/L Reference standard number IN SS Note. For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) ,,5353 0.50 O-1 r 1 IOa. a 0/o Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number r/bmgL SBlank (should be= <0.10 mg[L)
- 4.
~ 4.. -0.0~ L ",6. 0i O\\M WY IaI_ 0 1,,1,1-deirD r-cs
- ctx,
,,)a ( 0 o I.O)ox-C oOq 06%11. V*- Tk4q .I-D SoN-v,,n ,Ioc*or £.o.occoq3* s QN-ENT I. ¢jC' CnW2r3 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 ._number (mg/L) (mg/L) (acceptable range = 90 to 110%) INSSYDc 0.50 0.53 '1 1 o 0 7 °/ I 110 Reviewed by I i A Date reviewed
Environmental Testing Solutions, Inc. Page 13a Paae I of 3 Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst r. Date analyzed Iodide reagent: -ýT r4-ZaI's3 Acid reagent: T."3N(Zr, r'7 Calibration: 0.10 mg/L 1.00 mg/L Reference standard number T -5'ý 5 3 -TNSS--XS Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) --TNSS353 0.50
- 0. 5'.t. 4 o8.-8 %°j Sample measurements.
Sample Sample ID Sample characteristics Residual chlorine number (mg/L) -Blank (should be=< <0.10 mg/L) 40{- V~. '~> OL)8 C. OU~ 111~ S~eeoxa-00 7 c(ecv~ ý-DCue -~-~ OO8 b 0l q,o2-Sd*-e - 'NT-U.P Clco+"OpC.,N --t.o OoW0o ýýOe Cla o koO.ccoiq3
- O I 1113
____\\___o_*_CcA-e.v
- o colcw-Lo.ooNc'lor S-0.50.
o ao, O J1 5tlW' Sote.,ti ,cxt,.s sttqý'tu C 0 ttral:xvtc'*' btuIl 11"4.0 Dt'trt. 4-o ,o19 .0(0-
- 1. 8-0) co Q)
/.o0c14a Note: AI sAJl*a~mples were an'*alyzze~d in excess of EPA recommended holding time (IS minutes)3u~nle~ss "otherwise noted. . oc q' Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS =MV / TV x 100 n umnber (rag/L) (mg/L) (acceptable range = 90 to 110%) -L'q*330.50 o-qq3 J00./0 Reviewed by III' ZIIZ2 Date reviewed L2-ig> J
Environmental Testing, Solutions, Inc. Page 3' Page 3, of-3O Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst U93 Date analyzed I t Iodide reagent: .NI.'5 Acid reagent: -NrZaqr-7 Calibration: mg/L 1.00 mg/b Reference standard number "XNSS 3 3 I-SS353 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (rag/L) (acceptable range = 90 to 110%) Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]) x 100 number (mg/L) (acceptable range= 10%) 1 4Duplicate D~< 4j) D1
- 0.
Ue Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) 4~7-,P~t~V Blank (should be =< OtO mg/L) Y (13b1,6. 4 t ovYAn CoIeet Nc, ayO r t3O' 40 l __"___-_..L .ooia0, D101118-07 SYIAAA-ýA _IcytdN*",. ýA*Ckv, loop Atlt\\ow CC.o y 0l b8.co0
- .~
um sC, clfiiv* r-zO to\\ov-4CO~OG~f83 O~oll6 c cc~ iouxj fox- ~ 0.000433* Ibte t8-eua 0 "frox O{J116. lz lCw,.pi eJ~Ne %le &%, 1 cJLw _.c).O__ I Note: All samples were analyzeb in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) Reviewed by Date reviewed
Environmental Testing Solutions, Inc. Page 3 Page 3 of 3 Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accurnet Model AR25 pHi.on Meter Analyst U943 Date analyzed i1E J Iodide reagent: T__RB3S Acid reagent: Calibration: 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg[L) (acceptable range = 90 to 110%) ReeeceTu vau U1 Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number ( mg/L) number Blank (should be= <0.10 mgIL) r &ol Ill. lo "IXI t-5Gmt'-T NT ctecix, *.p cbL'vY L.O.O0&b(O Note. All samples were analyzed in exces -PA recommended holding time (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS MV/TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%) 0.50 OCicL q Q. /0 Reviewed by l/' IZ"- Date reviewed l-b
Sequoyah Nuclear Plant Biomonitoring November 14-21, 2006 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc. Pim eph ales prom elas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 K I I I I I I I I I I I I I USEPA Control Limits (+/-+2 Standard Deviations) I I I I 0.8 H 0.7 F 0.6 [ 0.5 I I I I I I I I I 1).. I1~2 1.0 0.8 0.6 0.4 I I I I I I I I I I I I I I I I I I USEPA Warning and Control Limits (75th and 90th Percentile CVs) .I l 0.9 I I I. I I I I I I I I I I I Laboratory Warning and Control Limits (10 and 25 Percentile CVs) 0.8 [ 0.7 F 0.6 0.5 0.4 Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC 25 ) Warning Limits (mean IC 25 +/- SA.10 or SA.75) Control Limits (mean IC25 +/- SA25, S A.90, or 2 Standard Deviations)
Environmental Testing Solutions, Inc. Pimeph ales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Test number Test date 7-day ICs CT (g/L KCI) (g/L KCI) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 12-06-05 0.61 02-07-06 0.67 03-07-06 0.61 03-14-06 0.53 04-04-06 0.57 04-18-06 0.64 04-18-06 0.61 05-09-06 0.57 06-06-06 0.45 06-13-06 0.56 07-11-06 0.61 07-18-06 0.61 08-01-06 0.64 08-08-06 0.67 08-15-06 0.49 08-22-06 0.65 09-19-06 0.61 10-10-06 0.60 10-17-06 0.64 11-14-06 0.54 0.64 0.63 0.61 0.60 0.61 0.61 0.60 0.59 0.58 0.59 0.59 0.59 0.60 0.59 0.59 0.60 0.60 0.60 0.60 State and USEPA Laboratory Laboratory S Control Limits SA.10 Warning Limits SAZS Control Limits SA. 5 s CT-2S CT+2S CT-SA,, CT+S 5,o CTc-SSa5 CT+S,5 s 0.04 0.56 0.72 0.08 0.57 0.72 0.13 0.51 0.78 0.24 0.03 0.57 0.70 0.08 0.56 0.71 0.13 0.50 0.77 0.24 0.06 0.50 0.72 0.07 0.54 0.68 0.13 0.48 0.74 0.23 0.05 0.50 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.05 0.51 0.71 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.05 0.52 0.70 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.04 0.51 0.69 0.07 0.53 0.67 0.13 0.48 0.73 0.23 0.07 0.45 0.72 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.06 0.46 0.71 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.06 0.47 0.71 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.06 0,47 0.70 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.06 0.48 0.71 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.06 0.48 0.71 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.06 0.47 0.72 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.06 0.47 0.72 0.07 0.52 0.67 0.12 0.47. 0.72 0.23 0.06 0.47 0.72 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.06 0.48 0.71 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.06 0.48 0.72 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.06 0.48 0.71 0.07 0.52 0.67 0.12 0.47 0.72 0.23 USEPA USEPA Waning Limits S,. 9o Control Limits CT - S,., CT + S,,, CT - SA.q CT + S,90 CV 0.40 0.39 0.38 0.37 0.38 0.38 0.37 0.36 0.36 0.36 0.36 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.37 0.89 0.29 0.35 0.87 0.28 0.35 0.84 0.27 0.33 0.83 0.27 0.33 0.84 0.27 0.33 0.84 0.27 0.33 0.83 0.27 0.33 0.81 0.26 0.32 0.80 0.26 0.32 0.81 0.26 0.32 0.81 0.26 0.32 0.82 0.27 0.33 0.82 0.27 0.33 0.81 0.27 0.32 0.82 0.27 0.33 0.82 0.27 0.33 0.82 0.27 0.33 0.83 0.27 0.33 0.82 0.27 0.33 0.93 0.06 0.92 0.05 0.88 0.09 0.87 0.09 0.88 0.08 0.88 0.07 0.87 0.07 0.85 0.11 0.85 0.11 0.85 0.10 0.85 0.10 0.86 0.10 0.87 0.10 0.86 0.11 0.86 0.11 0.86 0.10 0.86 0.10 0.87 0,10 0.86 0.10 Note 7-d IC25 = 7-day 25% inhibition concentration. An estiniation of the concentration ofpotassium chloride that would cause a 25% reduction in Pinsephales growth for the test population. CT = Central tendency (msean IC25). S = Standard deviation of the IC 25 values. Laboratory Control and Wanring Limits Laboratory control and warning limits were established using the standard deviation of the IC2 5 values corresponding to tde 10th and 25th percentile CVs. These ranges are nsore stringent than the control and wansing limits reconmmended by USEPA for ilse test msethod and endpoint. S,.o = Standard deviation corresponding to the 10th percentile CV. (S, 0 = 0.12) SA25 = Standard deviation corresponding to the 25th percentile CV. (SA25 =0.21) USEPA Control and Warning Limits SA.s = Standard deviation corresponding to the 75h percentile CV. ( SA75 = 0.38) Sx9O = Standard deviation corresponding to the 90th percentile CV. (SAO = 0.45) CV = Coefficient of variation of the IC 25 values. USEPA_ -2000. Undestanding and Accounting fnr Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinination Psograru. EPA-833-R-00-003. US EnviromentalPiotection Agency, Cincionati, OH. Organis.tt aotaed from Aquaifc BioSyste,. inc.1 11-14-06
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Pimeph ales promelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test number Test date Control Survival Control Mean Growth CT CV CT MSD PMSD CT for Control Growth (%) (mg/larvae) (mg/larvae) for Control Growth CV (%) (%) for PMSD (%) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 12-06-05 02-07-06 03 06 03-14-06 04-04-06 04-18-06 04-18-06 05-09-06 06-06-06 06-13-06 07-11-06 07-18-06 08-01-06 08-08-06 08-15-06 08-22-06 09-19-06 10-10-06 10-17-06 11-14-06 100 100 100 100 100 100 100 100 100 97.5 100 97.5 100 100 100 100 100 100 100 100 0.626 0.711 0.774 0.745 0.766 0.621 0.668 0.841 0.783 0.709 0.673 0.623 0.746 0.613 0.765 0.768 0.699 0.685 0.781 0.737 0.669 0.704 0.714 0.724 0.707 0.702 0.719 0.726 0.724 0.720 0.712 0.714 0.707 0.711 0.714 0.713 0.712 0.716 0.717 .7.1 7.8 8.4 9.7 11.5 9.7 13.3 7.5 5.9 8:2 2.5 17.1 10.5 10.4 8.0 8.7 12.7 5.1 7.3 5.3 7.5 7.8 8.3 8.9 9.0 9.7 9.4 9.0 8.9 8.3 9.1 9.2 9.3 9.2 9.2 9.4 9.1 9.0 8.8 0.06 0.09 0.10 0.14 0.08 0.09 0.10 0.12 0.08 0.16 0.09 0.10 0.13 0.08 0.16 0.12 0.12 0.10 0.15 0.10 10.0 13.2 13.3 18.9 10.7 13.8 15.4 14.4 9.6 22.0 13.1 16.6 17.0 13.6 20.4 15.5 17.2 14.0 18.7 13.4 11.6 12.2 13.8 13.2 13.3 13.6 13.7 13.2 14.1 14.0 14.2 14.5 14.4 14.8 14.8 15.0 14.9 15.1 15.0 Note: CV = Coefficient of variation for control growth. On average, the CV for control growth is 8.8% in Environmental Testing Solutions, Inc. Pimephales chronic toxicity tests. Lower CV bound determined by USEPA (10a percentile) = 3.5%. Upper CV bound determined by USEPA (90' percentile) = 20% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.0% from the control. Lower PMSD bound determined by USEPA (10' percentile) = 9.4%. Upper PMSD bound determined by USEPA (90"' percentile) = 35%. CT= Central Tendancy (mean Control Growth, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatic BioSystems, Inc. 11-14-06
Environmental T'esting Solutions, Inc. Pimephales promelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25 I I I i I I I F I I I I I I I I I I I I I I I 1.00 0.75 .-..- 0.50 0.25 IN r USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I r I I I I I I I I i I I T I 1 I I I I I I~ I I I I I o 20 30 Kentucky Acceptance Limi 20 H I I I I I I I I .t (< 30.0%) ~2 7 10 0 [ [ I I I I I I I I I I I I I I I I I 40 30 20 10 S I. tI i I I I I I I I USEPA Upper PMSD Bound (9 0th percentile < 35.0%) I I I I I I I 1 I t i I I I i t i t i I I I I I O 1 1 1 1 1 1 1 1 1 1 1 1 1 I Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)
Environmental Testing Solutions, Inc. Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephales promelas PpKCICR Test Number: jl Dilution preparation information: Comments: KCI CHM number: C.AA -Z(-*- Stock preparation: 50 g KCI/L: Dissolve 50 g KCI in t-L Deionized water Dilution prep (mg/L) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 18 Diluent volume (mL) 994 991 988 985 982 Total volume (mL) 00 1000 1000 1000 1000 Test organism information: Test information: Organism age: "2z. -IS -Mn "q IS R0,aL.k OLJ3 Randomizing template: !%*,?,e... Date and times organisms 1ý- I-t-o.. 1,4oo -T-o Ii to Incubator number and were born between: shelf location: Organism source: MkS& *,tu4 , It-LA-o1P Artemia lot number: Yl'512Z4 0 Transfer bowl information: pH SU Temperature 'C Total drying time: ZA.zs-0 S "i4,Z Date/Timein: ti-"2-ok, L!'5o Average transfer volume: Date/Timeout: l"z-o, 1,Io(o Oven temperature: (.n" C, Daily feeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time renewal, or batch used time termination time 0 0 W-L3/4-O* ~
4i
,OO tqq0 II-i-z.-eA,,. (' I l\\-kcwcto I bw::: 'AO Iq -1 w rz -o (J 2" 2 t\\11 I tcrocQ \\IoO %00og I-12.-d , -C 3 l k 1 -O 6 /0 0 5 1 ( 0 1 0 A .1 0 t I t - K' -0 (,, / 4 ooW_ 10o 01L 1( 00_ -_ -o_____ 5 I - %-0 o b I kro Q..- 1% z 9I 1\\t s-o ' (3 6 11-20-01. ORSSV13( 2 t i-w.o c 7rt~ ?, - '7.-43q-- Control information: Acceptance criteria Summary of test endpoints: % Mortality: Oo <20% 7-day LC50 -12S. L Average weight per initial larvae: 0, 11 )1 NOEC '.30 Average weight per surviving larvae: 0-13"1 -0.25 mg/larvae LOEC _3_0 ChV I < 300 iCh I-30
Environmental Testing Solutions, Inc. Page 2 of 5 Environmental Testing Solutions, Inc. Page 2 of 5 Species: Pimeph ales prom elas PpKCICR Test Number: I Survival and Growth Data Day Control 300 mg KCULL 450 mr KCI/L A B C D E F G H I J K L 0 0to O /to /0 /CQ O /0 10 10 10 '0 /0 1 /0 /0 /0 /0 /0 /0 /D /0. /0 /0 10 '0 2 10 Io 1 C I0 Q O t o /0 1 0 /0D /0 /0 30/0(0 0 / t0 /0 10 /0 /0 /0 3 4 O / D 1O /10 10 /0 10 /0 /0 01 5 /0 10 lo /0 "0 10(0 C0 0 9} 6 / 0* 0E 1O C) I -) 1 1 ) I 0 /0 /0. 7 SKsC 10 /O 0 /0 10 10 10 C) 0 10 /o 1 6/D (0 /0 /0 (0 /t /0 /0 I0 IO '3: 1* A Pan weight (mg) T ray c olor code:: R e W
- 13.1 o 1q.8 3 ta S A 13.-9 8 15. V ý 13.' 70Q 14. 5( o Nt.SO 1q, 3 3j N -1 0 1 5.-V 7 I* 13 W Analyst: LMB B = Pan + Larvae weight (rag)
Analyst: a.._9 O09Q an.1o ao.o'i ao.ýj a(.Oq lq.4q ;jjl.4 a*. o,. aO.MIS
- a.
.l I l;,.cI Larvae weight (mg) =A - B Weight per initial number of larvae (mg) = C /Initial number of larvae A" 01 A 1-7 AV A. A O" -V AY 0' -3 0" Average-Percent weight per reduction initial from control number of (%) larvae (mg) I 0. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r =.decanted and returned, w = wounded. Calculations and data reviewed: Comments:
Environmental Testing Solutions, Inc. Page 3 of 5 - iý Species: Pimeph ales promelas PpKCICR Test Number: IOS Survival and Growth Data Day 600 mg KCI/L 750 mg KCI/L 900 m KCI/L MN 1 0 P Q R S T U V W X 0 t o /0 /0 1 O 10 IO. /0 /0 10 /0 /0 A,~ l0 "1'4 G q A"' I ',. _1
- 5 s Z_
2 10 Cý 5 5 _ _-_ _to .3 2-4g 0 to S -2!4' a '4 2 3 0o -1 S S* Q Z t-2 -L.4 7 Lks 2 -1 £- -l t '4 z_ A = Pan weight (mg) Tray color code:: O.e3 I2T 14s.3%q a 13.19 I.3"1 13.3S I4.31 3Q 13. 1394 :'.15so l"4.04 13.13 IS.I9 Analyst: { -. B = Pan + Larvae weight Anlyt:g) 1:* ir'. b I(-x0 1q.,79 1*CSa /qqq IS.,() [(O.qqj ISbI IS.Sc 1!5.3t 15.9,. Larvae weight (mg) = A - B Weight per initial number of larvae (mg) Ali ~ ~ = C / Initial number of larvae 01 0,
- 11)
- 0.
10, 1
- ~
v 0' Average Percent weight.per reduction initial from control C). SO-number of (%) larvae (mg) Comment codes: c = clear, d = dead, fg fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewedi
Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promnelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR # 145 (#105 at 351 Depot St.) Test dates: November 14-21, 2006 Reveiwed by: -c Concentration (.g/L Replicate Initial number of lilnberf A - Pan weight (ng) B - Pan Lar.a-Lara-wight (rag) Weight / Surviving Mean weght/ Coefficient of variation Weigh( 5 Initial number Mean survival Mean weight/ Initial Coefficient of.ar.alsio Perent reduction from KCI) Ia.- larae deight (.ag) A - B number of larvae (ag) Survlving number of r Otiarae (mg) (%) number of lar 1%) onorol (%) larvae (nag) (mmhrva. (%1 (ang) A 10 10 13.16 20.90 7,74 0.774 0.774 CB 10 0 14.83 22.20 7.37 0.7.737 0737 5.3 737 100.0 0.737 5.3 Not applicable C 10 10 12.52 20.07 7.55 0.755 0.755 D 10 10 13.78 20.61 6.83 0.603 0.693 E 0 10 15.82 2104 5.22 0.522 0.522 300 F 10 10 13.72 19.49 5.77 0.577.0.619 13.5 0.577 100.0 0.619 13.5 16.0 G 10 10 14.56 21.42 6.86 0.686 0.686 H 10 10 14.50 21.42 6.92 0.692 0.692 1 10 10 14.33 21.05 6.72 0.672 0.672 450 J 10 10 14.10 20.41 6.31 0.631 0.652 83 0.631 95.0 0.620 9.9 16.0 K 10 9 15.67 22.11 6.44 0.716 0.644 L 10 9 13.60 18.91 5.31 0.590 0.531 M 10 7 13.34 17.05 3.71 0.530 0.371 600 N I0 8 14.82 19.85 5.03 0.629 0.674 20.5 0.503 75.0 0.505 19.4 31.6 O 10 7 13.18 19.20 6.02 0.860 0.602 P 10 0 14.37 19.79 5.42 0.678 0.542 Q 10 5 13.38 16.52 3.14 0.628 0.314 750 R L0 5 14.34 17.94 3.60 0.720 0.649 7.3 0.360 50.0 0.324 17.1 56.0 S 10 6 14.37 18.10 3.73 0.622. 0.373 T 10 4 13.94 16.44 2.50 0.625 0.250 U 10 2 14.50 15.81 1.31 0.655 0.131 900 V 10 3 14.04 15.59 1.55 0.517 0,659 15.5 0.155 20.0 0.126 28.7 02.9 W 10 2 13.93 15.38 1.45 0.725. 0.145 X 10 1 15.19 15.93 0.74 0.740 0.074 Dunnett's MSD value: PMSD: 0.0987 13.4 MSD = Minimums Significant Difference PMSD = Percent Minhinuns Significant Difference PMSD is a measure of test precision. The PMSD is the ousnimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environunental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.0% fromi the control (determined through reference toxicant testing). Lower PMSD bound determined by USEPA (10th percentile) = 9.4%. Upper PMSD bound deteriniased by USEPA (90th percentile) = 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pmnephales growth in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Underthe National Pollutant Discharge Elimination Program. EPA-933-R-00-003. US Environmental Protection Agency, Cincinnati, OH. Organisms obtainedfrom Aquatic BioSystems, nc. 11-14-06
Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 1111412006 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date. 11/21/2006 Lab ID. ETS-Envir. Testing So. Sample Type: KCL-Potasshure chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimnephales proenelas Comnments Conc-mgt/L 1 2 3 4 D-Control 1.0000 1,0000 1.0000 1.0000 300 1.0000 1.0000 1.0000 1 0000 450 1 0000 1.0000 0.9000 0.9000 600 0.7000 0.8000 0.7000 0.8000 750 0.5000 0.5000 0.6000 0.4000 900 0.2000 0.3000 0.2000 0.1000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Couc-mgl/L Mean N-Mean Mean Min Max CV% N Sum Critical Resp Number D-Conteol 1.0000 1.0000 1.4120 1.4120 1.4120 0000 4 0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 450 0.9500 0.9500 1.3305 1t2490 1.4120 7.072 4 14.00 10.00 2 40
- 600 0.7500 0.7500 1.0492 09912 1.1071 6.383 4
10.00 10.00 10 40
- 750 0.5000 0.5000 0.7854 0.6847 0.8861 10467 4
10.00 10.00 20 40
- 900 0.2000 0.2000 0.4572 0.3218 0.5796 23.087 4
10.00 10.00 32 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates nornmal distribution (p > 0.01) 0.927304924 0.884 -0.11783462 -0.07299576 Equality of varance cannot be confotmed H*pothesis Test (I-tail, 0.05) NOEC LOEC ChV TU Steers Many-One Rank Test 450 600 519.6152423 Treatmnents vs D-Control Maximum Likelihood-Probit Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 8.218545127 1.18554237 5.894882038 10.54220822 0 0.510637231 7.814727783 0.92 2.862395394 0.121676037 3 Intercept -18.5247257 3.373327762 -25.1364483 -11.9130032 TSCR 1.0 // Point P'robits mg/L 95% Fiducial Limits 0.9 ECOI 2.674 379.6073413 295.2835229 439.0208562 1 EC05 3.355 459.468902 383.559901 511.8507084 0.8-ECIO 3.718 50&.6994638 440.1419331 556.5132553 0.7 ECI5 3.964 544.8617129 482.2758146 589.6651074 EC20 4.158 575.4270324 517.9312937 618.2379899 00.6 EC25 4.326 603.012277 549.8515822 644.7448404 00.5 EC40 4,747 678.5301092 633.4494993 723.2887018 o. ECS0 5.000 728.4427003 683.7392443 781.8840101 0.4 EC60 5.253 782.0268659 733.1090911 850.8899764 0.3 EC75 5.674 879.9634494 814.8968753 989.3037695 0.2 EC80 5.842 9221477985 848.0460517 1052.488559 EC85 6.036 973.8779028 887.6041206 1132.258219 6.1 EC90 6.282 1043.108519 939.1191681 1242443569 00 EC93 6.645 1154.874127 1019.754797 1427.556739 1 1o too 1000 10000 EC99 7.326 1397.83602 1187.558721 1856.463025 Dose mg/L Dose-Response Plot 0 Organeisms obtained from Aquatic BioSystems., Inc. 11-14-06
Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Starn Date: 11/14/2006 Test ID: PpKCICR Sample ID: REF-Ref Tomsicant End Date: 11/21/2006 Lab ID: ETS-Envir. Testing Sol Sample Type: KCL-Potassiumn chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Corismeests: Conc-rng/L 1 2 3 4 D-Control 0.7740 0.7370 0.7550 0.6830 300 0.5220 0.5770 0,6860 0,6920 450 0.6720 0.6310 0.6440 0,5310 600 0,3710 0.5030 0,6020 0,5420 750 03140 0.3600 0.3730 0.2500 900 01310 0.1550 0.1450 0.0740 Transform: Vntransformed I-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mlean D-Control 0.7373 1.0000 0.7373 0.6830 0,7740 5,316 4 0.7373 L.0000
- 300 0.6193 08399 0.6193 0.5220 0(6920 13.508 4
2.605 2.180 0.0987 0.6194 0.U401
- 450 06195 0.8403 0.6195 0.5310 0.6720 9.916 4
2.600 2.180 0.0987 0.6194 0.8401 600 0.5045 0.6843 0.5045 0.3710 0.6020 19.400 4 0.5045 0.6843 750 0.3243 0.4398 0.3243 0.2500 03730 17.146 4 0.3243 0.4398 900 0.1263 0.1712 0.1263 0.0740 0.1550 28.671 4 0.1263 0.1712 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution(p > 0.01) 0.924493849 0.805 -0,53262068 -0.95050657 Bartlett's Test indicates equal variances (p 0.50) 1,389453173 9.2103405 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MISB MSE F-Prob df Danett's Test <300 300 0.098731115 0.133918094 0.018526083 0.004102278 0.043841291 2, 9 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05* 93 82 47.73 51.72 346.70 2.4121 1Cl0" 187.64 73.50 103.45 609.95 1.4317 IC15" 281.45 98.11 155.17 615.17 02127 1.0 1C20 488.62 75.26 138.89 622.17 -1.2522 0.9 IC25 536.75 42.15 421.08 668.52 0.2086 IC40 651.72 29.45 524.79 716.72 -1.1008 0 ICS0 713.07 20.57 644.68 778.29 -0.1493 0.7 indicates IC estimate less than lte lowest concentration "o 0.6 0.5-0*.4 0.3 0.2 0.1 0.0 0 200 400 600 800 1000 Dose mg/L Dose-Response Plot 1-talt, 0.05 level of significance U 63 Organi.sms" obtained from Aquatic BioSy.stems, Inc. 11-14-06
Environmental Testiniz Solutions, Inc. Page 4 of 5 EnviometlTetn Sou "os ,nPge4o Species: Pimephalespromelas PpKCICR Test Number: N C* Daily Chemistry: Day 01 2 Analyst A-I ALY'- V' t-- Concentration Parameter CONTROL pH (S.U.) .A09-94.61 5 .2-4 DO (mg/L)62-- Conductivity 3R 306 (ýImhoslcrn) 3I Alkalinity (mg CaCO3/L) Hardness (mg CaCO 3/L) Temperature (°C) pH (S.U.) "1.31,4 q..39 DO (mg/L) 8.2 6,o 611 300 mg KC/L Conductivity (imnhos/cm) .43 Temperature 1 z L (oc) _4. -'___.7 PH (S.U.) 9__57.- 'i5 Ali ____2 9q-24 .DO (mg/L-)81 450 'rg KCI/L Conductivity (Hrhos*cm) /060 /5b 113o T em perature -L. - I- -4A t (oC) 2q.
- pH (S:U.)
.L162 -"-5 55 q, 9.31 DO (mrg/L) .Z.3 0,o
- ,i
).Z 600 mg KCI/L Conductivity q1D (ýimhos/1m) o Temperature PH (s.u.) DO (rag/L) N.0
- . I 750 mg KCI/L Conductivity
( mprhos/cm ) "q. Temperature -(0A (OC) 2q.5 2+9f__ pH (S.U.) '4icqS) 11 1-0,4 1 q 11 !z5l 1 ~I ~ 1 DO (mg/L) 900 mg KCI/L Conductivity (*im.hos/cm) Temperature (0C) 1930 1q 2 q."7 2q., 2'7 1q. I -2q. e ZL_5 Initial Final Initial Final Initial Final STOCK Conductivity (ptmhos/cm)
I Environmental Testing Solutions, Inc. Page 5 of 5 Environmental Testin2 Solutions. Inc. Page 5 of 5 Species: Pimephlales promelas PpKCICR Test Number: 10S ~Day Concentration Parameter CONTROL pH (S.U.)
- .2La
%9q '4 63 1 e,6 Conductivity (iimhos/cm) Alkalinity 6 0 (mg CaCO3/L) Hardness (mg CaCO3/L) Temperature _ _ _ _ (°c) 2_____ pH (S.U.)
- q.
q -_2 !T1 3(a M.?t '4.5-DO (mg/L) "6 i IDA o '4 2-300 mg KC!/L Conductivity 21 1 (pmhos/cm) r5 Temperature 1 9 Z 214 q ___.q _ _ _ _ (oc) _____,7 pH(S.U.) 9.-*fl q.3, q.o q._L 9.6. __ DO (mg/L) (. IA..0 450 mg KCI/L Conductivity (Imhos/cm) ,) 10 Temperature 7.q~ Z t-." (0 c) Ilk__ Is__ _____S pH, [SU.
- c.
- q. 2
- q. 29 q-60 9,o*
-;6.40 600mgKCfL DO (mg/L) 9,0 9.6 G (0e .1 2.Zz Conductivity (prmhos/cm) Ir01q toIbo Temperature Z4i zq7 4:4.2A2'1 7J 24(- 2'47Z2 H(S.Uo ) _q t.1a z q J 1 'v7_ j qh DO,(mg/L) U 750 mg, KCIfL Conductivity r-oIao (!Imhos/cm) RO 0-1 Temperature 1 1 2S
- 2.
qq 2q. 7q, - 24.6 iq.7-L. (TC) j T__ 7__ L__ I__ PH (S.U.) 9, -?") 1,
- 174, 39
/a5-I Qý IIe 'J--57% I .qn r+,5,5 1 900 mg KCI/L DO (mg/L) Conductivity (*-mhos/cm) Temperature (0c) 2.4.5 V2..1 I ZC1.. 2 q.q Initial Final Initial Final Initial Final Initial Final STOCK Conductivity I_(t_[ nhos/cm) m
rnwvironmental I esting olutions, Inc. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 I Control I I I 'ations) USEPA Control Limits (+ 2 Standard Deviations) 1.10 F 1.08 1.06 1.04 2.5 ~i I ~ I I U U I I I I an I C I ( a I 1 Pecni l C s) USEPA Warning and Control Limits (75t and 90t Percentile CVs) 2.0 H 1.5 H 1.0 F-0.5 1.4 1.3 1.2 1.1 I' Ith I I K I I I I I I I th Laboratory Warning and Control Limits (10 and 25 Percentile CVs) I I I I ItI I I I I I I I I I 1.0 0.9 0.8 k)'3 IZb j)G 4ý6 iýb Iýb ý05 ZS Qýs zln C)b Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC 25) Warning Limits (mean IC 2 5 +/- SA.10 or SA.70 Control Limits (mean IC 25 +/- SA.25, SA.90' or 2 Standard Deviations)
Environmental Testing Solutions, Inc. Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC25 CT S Control Linits "SAA Warning Linits SA.2k Control Linits SA-75 Warning Limits SA.90 Control Limits CV (g/L NaCI) (g/L NaC1) CT-2S CT+2S CT-Skio CT-Sis CT - SA.ks CT + Sk.s CT - S,,5 CT + Skss CT - SA.9 CT + SA.0 1 06-07-05 1.05 2 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 3 07-12-05 1.06 1.06 0.01 1.04 1.07 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 4 08-09-05 1.08 1.06 0.01 1.04 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0,40 1.72 0.01 5 09-13-05 1.04 1.06 0.01 1.03 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.01 6 10-04-05 1.09 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 7 11-01-05 1.08 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18-0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.02 8 12-06-05 1.04 1.06 0.02 1.03 1.10 0.08 0.98 1.15 0.18 0.88 1,24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 9 01-10-06 1.08 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.02 10 02-07-06 1.07 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.02 11 02-07-06 1.06 1.06 0.02 1.03 1.10 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58. 1.54 0.66 0.40 1.72 0.01 12 03-07-06 1.05 1.06 0.02 1.03 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0,58 1.54 0.66 0.40 1.72 0.01 13 04-04-06
- 1.06 1.06 0.01 1.03 1.09 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 14
.05-02-06 1.08 1.06 0.01 1.03 1.09 0.09 0.98 1.15. 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 15 06-06-06 1.06 1.06 0.01 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 16 07-11-06 1.09 1.06 0.02 1.03 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 17 08-08-06 1.07 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 18 09-12-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 19 10-03-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0:48 0.59 1.54 0.66 0.40 1.72 0.01 20 11-07-06 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1,54 0.66 0.40 1.72 0.01 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (meas IC25). S = Standard deviation of the 1C21 values. Laboratory Control and Wanting Limits Laboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits reconmaended by USEPA for the test method and endpoint. SA10 = Standard deviation corresponding to the 10'h percentile CV. (SAS10 = 0.08) SA.25 = Standard deviation corresponding to the 25f percenlile CV. (SA25 = 0.17) USEPA Control and Warning Limits S.s = Standard deviation corresponding to the 75h percentile CV. (SA7s = 0.45) SA.95 = Standard deviation corresponding to the 90'h percentile CV. (SA90 = 0.62) CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimnination Program. EPA-833-R-00-003. US Environnimctal Protection Agency, Cincinmati, OR. 10.03.06
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test Tert Test date numb er Control Control Mean Survival Reproduction (%) (offspring/female) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 06-07-05 07-12-05 07-12-05 08-09-05 09-13-05 10-04-05 11-01-05 12-06-05 01-10-06 02-07-06 02-07-06 03-07-06 04-04-06 05-02-06 06-06-06 07-11-06 08 06 09-12-06 10-03-06 11-07-06 100 100 100 100 100 100. 100 100 100 100 100 100 100 100 100 100 100 100 100 100 30.4 30.4 31.1 28.3 27.9 27.0 28.4 32.6 29.2 30.7 29.9 28.8 27.0 28.6 30.3 29.0 28.6 30.9 32.3 31.0 CT for Control Mean Reproduction (offspring/female) 30.4 30.6 30.1 29.6 29.2 29.1 29.5 29.5 29.6 29.6 29.6 29.4 29.3 29.4 29.4 29.3 29.4. 29.5 29.6 CV CT MSD PMSD CT for Control (%) Reproduction CV (%) (%) for PMSD (%) 5.0 7.5 7.2 7.3 7.3 5.8 10.3 6.3 4.8 6.0 6.8 5.9 5.2 8.6 5.2 5.4 8.9 5.4 4.6 6.3 6.2 6.5 6.7 6.8 6.7 7.2 7.1 6.8 6.7 6.7 6.7 6.5 6.7 6.6 6.5 6.7 6.6 6.5 6.5 2.6 2.7 3.2 2.9 3.9 3.0 3.8 2.3 2.3 2.4 2.2 2.6 2.1 2.7 3.0 2.5 3.7 3.3 3.1 2.4 8.5 8.8 10.2 10.3 13.9 11.1 13.2 7.1 7.8 7.9 7.5 8.9 7.6 9.3 9.8 8.6 12.8 10.6 9.5 7.8 8.7 9.2 9.5 10.3 10.5 10.9 10.4 10.1 9.9 9.7 9.6 9.5 9.4 9.5 9,4 9.6 9.7 9.7 9.6 Note. CV-Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.5% in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity tests. Lower CV bound determined by USEPA (10 th percentile) = 8.9%. Upper CV bound determined by USEPA (90 ' percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA (10 th percentile) 11%. Upper PMSD bound determined by USEPA (9 0at percentile) = 37%. CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD) The lower and upperbounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 11-07-06
Lnvironmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 0 o c.~ 0~ o ~ 0 0 0 ~ 0-0 ~ ~ 0 00 40 35 30 25 20 15 40 30 20 10 40 30 20 10 Minimum Acceptance Criteria (> 15.0 offspring per surviving female) I I " F I I I I I .1 I North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%) I I I I I I I I I "* I I I I t I, I I I I I th USEPA Upper PMSD Bound (90' percentile < 37.0%) M* Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) .... Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)
Environmental Testing Solutions, Inc. Page I ot'6 Envronentl Tstig Slutons In. Pge Iot Sodium Chloride Chronic Reference Toxicant Test (EPA-821 -R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: S_* Dilution preparation information: Comments: NaCI CHM number: .A tA I"2z(3 Stock preparation: 100 g NaCI/l (dissolve 50 g NaCI in 500 ml deionized water) Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism information: Test information: Organism age: < 24-hours old Randomizing template: O-AA WP-Date and times organisms I\\I-o ,-L', T"TO 270072-- Incubator number and were born between: shelf location: Organism source: I o.3-V olA UK% I,Z3,4.S.,,, i o. vA '*.S. YCT batch: __-o_-__ Transfer bowl information: pH =.3 SU Temperature 7qCC Selenastrum batch: Daily renewal information: Day Date Test initiation, MHS water Analyst renewal, or batch used termination time 0 o0-1-06 10- -o 2 fl-o --o (.o I0 C0.21- ( 6o C -- 3____ .o. W-2.1-06 C, 5 11-12-06 10,34 o r-C- Control information: Acceptance criteria Summary of test endpoints: % of Male Adults:
- 7, 20%
7-day LC50 > i'joo % Adults having 3 d.Broods: /ot >80% NOEC I (____C __ % Mortality: (
- 7.
<20% LOEC t -LOD Mean Offspring/Female: ,33, 0 >15.0 offspring/female ChV A % CV: < 40.0 % IC25 ____,_._0
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: SS CONTROL Survival and Reproduction Data Replicate number Da 1 2 3 4 5 6 7 8 9 10 F7 Adult mortality __-__-_~L k L i- \\ 2 Young produced o O O Q C) I Adult mortality \\-- t k 3 Young produced C C) c Adult mortalit-y -I V -- V - 4 Young produced ,,rodu,,Z I [T 2?- ~ Adult mortality Adult mortality l L-J\\J_. lx..{QV K K 6 { Young produced 1*_
- )
Young produced 0 z'tI' )I C. Adult mortalit 7- -Yugroe
- t. (0 t
-'t A is-Total young produced 2-56 vO 6 2-0
- 2. z
- 52.
"2., z Cl Final Adult Mortality X for 3rd Broods j 7 Z j j Note: Adult mortality (L live, D dead) Concentration: % Mortality: 07.. Mean OffspringFemale: 3,0 600 mg NaCI/L Sun,'ival and Reproduction Data Replicate number
- Day, 2
3 4 5 6 7 8 9 10 I Young produced 0 C__) c_ C_ 0 C) j_ Adult mortality 3 Young produced c C LJ qLII U LI o __10 Adult mortality H-53_k SI-H 1 4 Youngproduced S_ [ S " _7 Adult mortality 6 Young produced 0~ t C:) 0 Q) C) 0f'f~ iii~ Adult mortality L
- Young produced C:
C) C)- i1 31-L5 6-ý- YngrdedAdult mortality L7"I_ l* I I" °* 7 [.Y oung produced I t,
- 1.
ýA " j% J~5 jS 4 I q t Total young produced -a Z-k Z) 2-53 o' 3\\ 3 3 Final Adult Mortality~ C Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: Mean Offspring/Female: 31 I % Reduction from Control: 3,1. o
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: S5 800 m2 NaCIJL Survival and Reproduction Data Replicate number Day 1 2 3 m l4 5 6 7 8 9 10 I Young produced 0 Q 93 2 Young produced 1D101 01 1 [ 1 1 0 C Adult mortality I I I L_[ I j__ 1 3 Young produced Li1
- 0) cO Adult mortality 4
[Young produced I, i* 'A I,[ Ljt" L0 Adult mortality I I J-- I
- xI.
- --I 4 [Young produced 0
1 17 Q I-Z0 Adult mortality L[ I_ 6 Young produced g Co_.iZ. loCD] Adult mortality _.k.. k_. 7 [ Young produced I.l tL.L 1-1 l,-o IS IS 19 I-7 I13 Total young produced z '30 t- '5 ) Final Adult Mortality k...- Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 0 "tý Mean Offspring/Female: 31.0 % Reduction from Control: 07. 1000 mg NaCJL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced Q 0 C 0 0 C_ G C_ Adult mortality 2 Young produced C)I Q o lCLoI OI c0 [ 0LO Adult mortality L_.- L,_ L[- I.__ 3 Young produced 1C)_o 0 0 _O c) [Adult mortality 14_-J\\ -jL~I-C I4 t__ 4 Young produced Adult mortality k._____ Young produced /0 o 0 I c I I Adultmortality I j(- I j* k 6 Young produced 1 C1 0 CW1_CT_0 UVCFU7 ] o [Adult mortality I 1 L-1ZL `( [ LjU U- \\, k--L. 7 f Young produced It-A 1, I to IS 1,S 1 6 I:S 13 1-7 1 Total young produced Zq -1 "2.4 2 k 31 Z,9 3Z 2-8 Final Adult tM ortality \\_ t-. C,.. k._ ý.. k... Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 070 Mean Offspring/Female: . 3o. % Reduction from Control:
Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: ý5S 1200 mr NaCIJL Survival and Reproduction Data Replicate number Day,_ 1 1 2 3 4 5 6 7 8 9 10
- 1 Young produced 0
0 0 C) 0 C 3 0 c) Adult mortality I [ 1
- L 2
Young produced 01 0 01 n Adult mortality Young produced Adult mortality Young produced 1 B_°- F Adult mortality. 6 [ Young produced CD_ q_"____ Young produced 11cI T I) 0 C) 0, lo R a0 Total young produced Final Adult Miortality '2J ~ T C Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: 07. Mean Offspring/Female: 9I % Reduction from Control: -1 o. V,7., 1400 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced C0 0_ C) ______ SAdult mortality 2 -
- . Young produced 0
- 3) 5!61J7*
1 80 10 10 Adult mortality I 3 Young produced C)_]O 1 o Iclo Adult mortality [ '- I _ I _-% I - -I - I _ 4 Young produced 1 Q 1 T 1 2 TV I Adult mortality i-t 5[Young produced 00 [ Adultmortality 1-i.:. I '_- [ 7 Young produced C0 0c ) Adultmortality I-- 7 Y oung produced 0 o o a Total young produced 7 15 0 0 \\fL_(
- 2.
L4 Final Adult Mortality t.- 1".. \\,- Note: Adult mortality (L = live, D = dead) Concentration: % Mortality: -z Mean Offspring/Female: 2-S % Reduction from Control: q9 1.i"
Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control Re licate number 1 2 3 4D y 1-6 7 8 1 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 5 5 5 4 5 5 4 4 4 47 5 10 0 0 0 0 0 0 0 0 0 10 6 0 13 10 11 12 12 9 13 10 10 100 7 16 15 15 15 13 17 18 .15 14 15 153 Total 32 33 30 31 29 34 32 32 28 29 310 600 mg NaCI/L Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 5 -5 5 5 5 4 6 4 4 47 5 0 11 0 11 13 0 0 0 0 0 35 6 12 0 10 0 0 13 11 11 10 12 79 7 16 18 14 15 17 15 15 14 18 14 156 Total 32 34 29 31 35 33 30 31 32 30 317 800 mg NaCI/L 800 1 Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 4 5 4 4 4 5 5 46 5 0 10 11 12 0 12 0 0 11 0 56 6 13 0 0 0 10
- 0.
9 10 0 10 52 7 14 14 17 16 15 15 19 16 17 13 156 Total 32 29 33 32 30 31 32 30 33 28 310 1000 mg NaCI/L Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 0 0 0 0 0 0 00 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 4 4 5 5 4 4 5 4 45 5 14 10 0 10 11 10 12 12 10 10 99 6 0 0 9 0 0 0 0 0 0 0 9 7 14 13 16 15 15 18 15 13 17 14 150 Total 34 27 29 29 31 33 31 29 32 28 303 1200 mg NaCI/L Re licate number Day 2 3 4 15J 6 7 8 910 Total 1 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 2 2 0 2 4 1 1 3 3 21 5 10 0 2 8 2 12 0 5 5 3 47 6 0 6 0 0 0 0 2 0 0 0 8 7 0 0 6 0 8 0 1 0 0 0 15 Total 13 8 10 8 12 16 4 6 8 6 91 1400 mg NaCI/L Re licate number Day 1 2 3 4 5 6 7 8 9 1 Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
- 3 0
0 0 0 0 0 0 0 0 0 0 4 2 0 0 0 1 0 0 2 2 1 8 S 5 0 0 0 0 4 3 0 0 0 12 6 0 3 0 0 0 0 0 0 2 0 5 7 0 0 0 0 0 0 0 0 0 0 0 Total 7 3 0 0 !1 4 3 2 4 1 25
Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #84 (#55 at 351 Depot St.) Test dates: November 07-14, 2006 Reveiwed by;,--JjtAvýý Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 1(%) (offspring/female)
- aIatO* t%)
cot (,) Control 32 33 30 31 29 34 32 32 29 29 100 31.0 6.3 Not applicable 600 32 34 29 31 35 33 30 31 32 30 100 31.7 6.0 -2.3 800 32 29 33 32 30 31 32 30 33 28 100 31.0 5.5 0.0 1000 34 27 29 29 31 33 31 29 32 28 100 30.3 7.5 2.3 1200 13 8 10 8 12 16 4 6 8 6 100 9.1 40.3 70.6 1400 7 3 0 0 1 4 3 2 4 1 80 2.5 86.9 91.9 Dunnett's MSD value: PMSD: 2.417 7.8 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a. whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.6% from the control. Lower PMSD bound determined by USEPA (10"' percentile) =11%. Upper PMSD bound determined by USEPA (90'h percentile) = 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Environmental Testing Solutions, Inc. Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 11/712006 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 11/1412006 Lab ID: ETS-Envir Testing Sol. Sample Type: NACL-Sodium chloride Sample Date ProtocoL FWCIH-R-EPA-821-R-02-013 Test Species: CD-Ceriodaplmia dubia Comments Cone-inglL 1 2 3 4 5 6 7 8 9 10 D-Control 32000 33.000 30.000 31.000 29.000 34000 32.000 32000 28.000 29.000 600 32000 34ý000 29ý000 31.000 35.000 33.000 30.000 31.000 32.000 30.000 800 32.000 29.000 33.000 32.000 30.000 31 000 32.000 30.000 33.000 28.000 1000 34 000 27.000 29.000 29.000 31.000 33.000 31.000 29000 32.000 28.000 1200 13 000 8.000 10.000 8.000 12.000 16.000 4.000 6.000 8.000 6.000 1400 7000 3.000 0.000 0.000 1.000 4.000 3.000 2.000 4.000 1.000 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N I-Stat Critical MSD Mean N-Mean D-Control 31.000 1.0000 31.000 28.000 34.000 6.270 10 31.350 1.0000 600 31.700 1.0226 31.700 29.000 35.000 5.958 10 -0.662 2.287 2.417 31.350 1.0000 800 31 000 1.0000 31.000 28.000 33.000 5.483 10 0.000 2.287 2.417 31.000 09888 1000 30.300 0.9774 30.300 27.000 34.000 7.469 10 0.662 2 287 2.417 30.300 0.9665 01200 9 100 0.2935 9.100 4.000 16.000 40 276 10 20.721 2.287 2.417 9.100 0.2903 01400 2.500 0.0806 2.500 0.000 7000 86.923 10 26.966 2.287 2.417 2.500 0.0797 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0 81748134 1.035 0.44619717 0.3712335 Bartlett's Test indicates equal variances (p = 0.18) 7.55277777 13.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDa MSDp MSB MSE F-Prob df Dusmett's Test 1000 1200 1095,44512 241677734 0.07796056 1738.96 5.58518519 1.6E-38 5, 54 Treatments vs D-Control Linear Interpolation (200 Resamples) Point mig/L SD 95% CL Skew IC05 1004 88208 51.0038904 825.635417 1014.14902 -2.5889 ICIO 1019.66981 5.96832498 1007.34438 1028,34195 -0.3117 IC15 1034.45755 5.61835965 1023 38686 1043.09093 -0.2720 1.0 IC20 1049.24528 5.40692453 103S.23354 1057.91483 -0.2188 0.9 IC25 1064.03302 5.35046783 1052.85837 1072.74883 -0.1422 IC40 1108.39623 6.09494531 1095.59506 1118.42537 0.2053 0.8 IC50 11379717 7.17272292 1123.85682 1151.46069 0.4115 0.7 0.6 C0.5' 0.4 02. 0.1 0.0 -0.1 0 500 1000 1500 Dose mg/L Dose-Response Plot 1-tail, 0.05 level of significance 11-07-06
Environmental Testing Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: -S Daily Chem istrv: ~~Analyst Concentration Parameter PH (S.U.) "-.(o DO (mg/L )Y 8-CONTROL Conductivity Qimhos/cm) _5 12_ Alkalinity (mg CaCO 3/L) Hardness (ma CaCO3/L) Temperature _(oC) 1G .o 0 -V -I I pH (S.U.)
- 2..
i .2__ k 9 DO (mg"L) 600 mg NaCI/L Conductivity (pmhos/cm) 0 .1490 Temperature z L () 'LS.2- .q O.'I _ _(_ _mhos/_ c m) I__ ,H (S.U.) 9.45" 2-J 9.z DO (m..L) 9 q,4 800 mg NaCI/L Conductivity (pmhos/cm) /go 1,7D o Temperature "wq.. 1. pH (S.U.) 9,10 ?.q
- 9.
1000 mg NaCI/L Conductivity 3o (pmhos/cm) Temperature (Zq,- zs,k
- z.
Zq. 6 ..H(S.U.) ~ r ~ 29 ~ DO (mg/L) 2 1200 mg NaC~fL Conductivity (4n-hos/cm) Temperature -q2-0z ~ L.- I (0c) PH (S.U.) I ?'q0 1).al ýq 3jL).qz g'?3 1 DO (mg/L) I q-1 1400 mg NaCI/L Conductivity (timho /cm) 13)10 i Temperature oCQ in0 a-1 nia Fi na Fia Final In~itial Final Initial WFinal STOCK 1 Conductivity I_(__ mhos/cm) /
Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR 4: -5*S _Day 3 4 5 6 Analyst Concentration Parameter H (S.U..9 9.s* o_.__ CONTROL Conductivity (ýimhos/cm) 3 ce 12. Alkalinity (mg CaCO3/L) Hardness (mg CaCO 3/L) Temperature pH (S.U.) ?.( '4.9!57 f _.2 '4.'1 1Iq b.O0 0 DO (mg/L) Q 600 mg NaCLL Conductivity (pmhos/cm) /5/0q20 Temperature (0c) '24. 9" "- 5 \\ z.! , *5 o2 -q *-q q"'. pH (S.U.) I6O DO (mg/L) S1 6 800 mg NaCIJL Conductivity (+/-mhos/cm) 1050 1320 1 e_5 Temperature I (0C ) \\ q --I. pH (S.U.) ,0 S-C'o 9.9j-4o 1'Th DO (mg/L) 811 A-1000 mg NaCI/L Conductivity (prmhos/cm) 940. T em p eratu re 1 a 1. 0 R _ (oc) __, _"2_. ______h pH (S.U.)
- 6.
z,1q 1,h 2.8 q.0-S.on-DO (mg/L) all "3",'7 ý'e (93*@1 8.3"7, 12 00 mg NaCI/L Conductivity Temperature ."1 " q,o _(C) ,Z_ pH (S.U.) 1.6 I 13 1 93A 11 4 1 1,1 1 q~ I e~--1 4C~ DO (mgL) 1400 mg NaCI/L Conductivity (Pmhos/cm) f zq)' -VS 2q r .32-C Temperature (°C) STOCK Conductivity I (Imhos/cm) I Initia~l Final IInitial Finalý Initial F!!Finti1 i~nal
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE SB-2A) SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard PARAMETER AVEF PH SAMPLE MEASUREMENT 00400 1 0 0 P PERMIT: EFFLUENT GROSS VALUE REQUIREMENT SOLIDS, TOTAL SUSPENDED SAMPLE c MEASUREMENT 00530 1 0 0 3/4PERMIT }R EQUIREMENTI} EFFLUENT GROSS VALUE R -1>M1O-I OIL AND GREASE SAMPLE MEASUREMENT 00556 1 0 0 .R... (' REQUIREMENTh EFFLUENT GROSS VALUE MO* FLOW, IN CONDUIT OR THRU SAMPLE 1. TREATMENT PLANT MEASUREMENT 50050 1 0 0 1PERMIT~ REP 'REQ'UIREMENT EFFLUENT GROSS VALUE REQUIREMENT
- M NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Fonmi Approved.
DISCHARGE MONITORING REPORT (DMR) (SUBR 01) 0MB No. 2040-0004 TN0026450 103 G F - FINAL PERMIT NUMBER [DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND NIT9RIN PERID EFFLUENT YEAR MO DAY MO DAY NO DISCHARGE From 061 11 t01] ToL0 j 113 t** O ICHRE NOTE: Read instructions before completinq this form. QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SA' EX OF IT A N A LY S IS MPLE "YPE RAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 7.1 8.2 12 SU 0 14 /30 THREE/ \\WEEK GRAB G RAB 3/4 6.0 MIMIMIRIM. 9.0k I. 4.*flE~IV* fl, 4~ 4
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I1 105 26 8 10 19 MG/L 0 5/30 GRAB <GRAB: 80V I 1250 AVG DAILY Mx LBS/DY r30.. 100< MO AVG IDAILY..MX-WEEKLY 59 <68 265/30 GA L2JY <5 ýL o 530 GA 925 BIY15 20MGLWEEKLY GRAB <AVG ~ DAILY MX MO AVG DAILY MX 325 1.584 03 MGD 0 30 / 30 TOTALZ TOTALZ ORT REPORT SEE PERMIT~ AVG ,..4. ,LYMXVf> 4 ~ 44 444-SAMPLE MEASUREMENT PERMITEN* REQUIREMENT SAMPLE MEASUREMENT PERMIT<c REQUIREMENTf SAMPLE MEASUREMENT REQUIREMENT 1< NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my - TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 06 12 13 Site Vice President information, the information submitted is,to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _LIN`TEROFFICLE SB-2A). SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOESI MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 11 107 G F-FINAL PERMIT NUMBER I DISCHARGE NUMBER METAL CLEANING WASTE POND MONITORING PERIOD EFFLUENT Form Approved. OMB No. 2040-0004 I YARI O A Y YA IM D44 AY From 1 j6i1j11 J To 1L06 111 1 a NO DISCHARGE W NOTE: Read instructions before comoletinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. {FREQUENCY SAMPLE EX OF TYPE ANALYSIS AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS 'I- -I I-I- I PH 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE 00556 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 12 SU REUPERMIT REQUIR~EMENT' 6.0. MINIMUM ><~MAXIMUM DAILY~. GRAB SAMPLE MEASUREMENT 19 PERMIT 30 MG/L "DAILY:- COMPFS ~REQ~iREMENT-, .~n 2 22 DAILY MX SAMPLE MEASUREMENT 19 REOUIREMENT, .2t ~15 K DAILY-MX MGIL 6A AILY-' 'I -GRAB-V PHOSPHORUS, TOTAL (AS P) SAMPLE 19******** 19 MEASUREMENT 00665 1 0 0 TAPEARMITSA Ii******** IMEASUIREMENT 1 EFFLUENT GROSS VALUE
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PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address PO. BOX 2000 (INTEROFFICE SB-2A). SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved. OMB No. 2040-0004 MONITORING PERIOD 1 EFFLUENT IYEAR IMO DAY YEAR MO DAY NO DISCHARGE 5a... From 061 101 To1 06 1 30_ NO R PARAMETER TEMPERATURE, WATER DEG. CENTIGRADE 00010 Z 0 0 INSTREAM MONITORING PH 00400 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED 00530 1 0 0 EFFLUENT GROSS VALUE QUANTITY OR LOADING QUALITY OR CONCEN TRATION NO. FREQUENCY SAMPLE EX OF TYPE MAXIMUM UNITS ANALY AVERAGE MAXIMUM UNITS MINIMUM AVERAGE SAMPLE MEASUREMENT 04 DEG C 04 DEG C DAILY -PERMIT-~ ~
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (.INTEROFFICE $8-2A) SODDY - DAISY TN 37384 Facilitv TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 I110 T F-FINAL PERMIT NUMER I DISCHARGE NUMBER RECYCLED COOLING WATER MONITORING PERIQD I EFFLUENT Form Approved. OMB No. 2040-0004 I YEAR I MO DAY ý YEAR I MO DAY I NO DISCHARGE From 06 [ 11 101 1 ToL06 1 11 30 NOTE: SC ARGea i cio NOTE: Read instructions before completinq this form. PARAMETER IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1 0 0 EFFLUENT GROSS VALUE IC25 STATRE 7DAY CHR PIMEPHALES TRP6C 1 0 0 QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY EX OF ANALYSIS SAMPLE TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS I I-I- 4 4 SAMPLE MEASUREMENT ,It,It 23 ..PERMIT ~REQUIREMENT ~j*AA A~v~ I> ~*** ~' 'I..
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment for knowing violations. Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVI OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 .(.INTEROFFICLE SB-2A) - SODDY - DAISY TN 37384 Facility TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 116 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING PERIOD EFFLUENT Form Appomed. OMB No. 2040-0004 YEAR MO DAY YEAR MO DAY From 06 11 01 To 0 11 30 NO DISCHARGE NOTE: Read instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY SAMPLE OF TYPE ANALYSIS 4 AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS W N 4 + + 4 DEBRIS, FLOATING (SEVERITY) 01345 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 1 0 0 ýEFFLUENT GROSS VALUE SAMPLE MEASUREMENT
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PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O BOX 2000 (INL~tTEROFFICjE S-2A---_) SODDY - DAISY TN 37384 FaciliiN TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) TN0026450 117 G PERMIT NUMBER DISCHARGE NUMBER MONITORING PERI YEARI MO i DAY I YEAR MO DAY From j 06111]fi l ! To 906 11 30 MAJOR FoIrm Approved (SUBR 01) OMB No. 2040-0004 F - FINAL BACKWASH EFFLUENT NO DISCHARGE KG NOTE: Read instructions before completin. this form. NCENTRATION NO. 1FREQUENCY SAMPLE EX OF TYPE MAXIMUM UNITS ANALYSIS ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CO AVERAGE MAXIMUM UNITS MINIMUM AVERAGE -V -+ 4 -4 -~ 1-4 i- -I DEBRIS, FLOATING (SEVERITY) 01345 1 0 0 EFFLUENT GROSS VALUE OIL AND GREASE VISUAL 84066 1 0 0 EFFLUENT GROSS VALUE SAMPLE MEASUREMENT 0 9A PASS=0 FAIL=1 0 1 /30 <SEER PERMIT VISUAL
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F~ SAMPLE MEASUREMENT PERMIT REQUIREMEN~T 0 94 YES=1 NO=O 0 1 / 30 VISUAL REPORT> MO -TOTAL SEE PERMIT VISUAL. SAMPLE MEASUREMENT PERMIT -REQUIREMENT SAMPLE MEASUREMENT 4.. + F...... PERMIT4 REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT SPERMIT REQUIREMENT ~ 4~ ~- SAMPLE MEASUREMENT F-.... PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my iI -direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the. Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _L(INTEROFFICE SB-2A SODDY-DAISY TN 37384 Facility TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 1 118 G I F-FINAL PERMIT NUMBER I DISCHARGE NUMBER WASTEWATER & STORM WATER ,MONITORING PERIOD I EFFLUENT Form Approved. OMB No. 2040-0004 LYEA I O I DYIYERIM IDA From L61111 1011 Tol L~Ill J39J NO DISCHARGE NOTE: Read instructions before completinq this form, PARAMETER OXYGEN, DISSOLVED (DO) 00300 1 0 0 EFFLUENT GROSS VALUE SOLIDS, TOTAL SUSPENDED QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. EX FREQUENCY OF ANALYSIS SAMPLE - TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS SAMPLE MEASUREMENT I I I 19 REQUIREMENTS .DAILY M MG/L TWICIEI."! GRAB., V-V'**: , EEK"-;"*;" I I SAMPLE MEASUREMENT Wk 19 00530 1 0 0 EFFLUENT GROSS VALUE PERMIT ,REQUIREMENT ,-.{1 "100 DAILY MX MG/L TVVICE/ WEEK-GRAB f, SOLIDS, SETTLEABLE SAMPLE
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REOUIEMENT-~- ~ ~ r SAMPLE MEASUREMENT MEASUIREMENT NAMETITLE PRINCIPAL EXECUTIVE OFFICER I Cariy under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer information, the information submitted is, to the best of my knowledge and belief, true, Priip EvirnmetalEngnee 423_843-6700_06_12_13 Site Vice President accurate, and complete. t am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE IAREA information, including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I D COEN COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1}}