ML053530164
| ML053530164 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 12/14/2005 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control |
| References | |
| Download: ML053530164 (200) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 December 14, 2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR NOVEMBER 2005 Enclosed is the November 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 jS25 Primed on ryed papw
PERMITTEE NAME/ADDRESS (Include Facility NameALocation ifDifferent)
Name TA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 __
iTEROFFIC1E B2__-
SODDY-DAISY TN 37384 Failitvy A-SEQUO AH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN026450 I l101 G
F - FINAL I
PERMIT NUMBER I I DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved.
OMB No. 2040-0004 MONITORING PERIOD I
EFFLUENT F YEAR i MO I
TY i M O
DA From 05 TO IT 05 I 11 30 ATTN: Stephanie A. Howard
- NO DISCHARGE [I -
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF is TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 20.4 04 0
30/30 MODELD CENTIGRADE MEASUREMENT 0010 Z
0 0
W7"-Ai" DE.C INSTREAM MONITORING D..
A PRm TEMPERATURE, WATER DEG.
SAMPLE 33.2 0
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>x-fX EFFLUENT GROSS VALUE REURE Z
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MA i
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<5 19 0
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0 0P\\
- 2 5
20 MG/L MO HL 2~
EFFLUENT GROSS VALUE MGALY MX v
FLOW, IN CONDUIT OR THRU SAMPLE 1563 03 0
30/30 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 0
In f
= VWP MGD
- t t
m CONTIN EFFLUENT GROSS VALUE D~ YM2Uoq"~
NAMEfTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that thb docment and all attachments were prepared under my I
TELEPHONE DATE direction or supervision in accordance with a system designed to *ssure that qualified personel Tn n n lIwAn I11 I
J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or parsons who manage the systm, or those persons dfirectly responsible for gatherig th Principal Environmental Engineer 423 843-6700 05 12 1
information, the information submitted is.to the best of my knowledge and belief, true, PiplnrmtEge 43___0_l1_4 Site Vice President accurate and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED.ORPRINTED Information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRNTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No dosed mode operation. CCW data for November 2005 is attached. Veliger monitoring information is attached.
EP Fr 33_ (RE P.viu ediion ma be use Page 1: of 2 EPA Fonrn 3320-1 (REV 3199)
Previous editions may be used Page 1 of 2
CCW TRENCH DatelTime Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 11/02/2005 @ 0745
<0.5 11/05/2005 @ 0050 KRR EPA 8015B CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 11/02/2005 @ 0740
<0.5 mg/L 11/05/2005 @ 0012 KRR EPA 8015B
Mean# ofWaerTep ZMean
% Settlers WaterTemp. Sample Date ZM~m' (C
Sample Date Mean# of Asiatic Water Temp.
LOCATION SUB Clams/mr3 (C)
LOCATION SAMPLE TYPE COLLECTED BY 07/06/2005 07/08/2005 07/13/2005 07/15/2005 07/20/2005 07/22/2005 07/27/2005 07/28/2005 07/29/2005 08/03/2005 08/05/2005 08/09/2005 08/11/2005 08/16/2005 08/19/2005 08/24/2005 08/26/2005 08/30/2005 09/02/2005 09/06/2005 09/08/2005 09/12/2005 09/16/2005 09/22/2005 09/23/2005 09/26/2005 09/31/2005 10/04/2005 10/07/2005 10/12/2005 10/14/2005 10/18/2005 10/21/2005 10/24/2005 10/28/2005 11/01/2005 11/03/2005 11/09/2005 11/10/2005 11/15/2005 11/18/2005 11/21/2005 11/23/2005 11/28/2005 12/01/2005 0
122 51 0
38 0
143 488 90 0
0 383 0
34 100 51 133 150 17 66 100 87 100 67 83 67 239 294 100 542 67 283 33 200 0
131 0
0 0
0 0
0 0
0 0
29 33 0
50 0
100 100 120 0
0 35 0
0 100 100 25 100 100 100 100 100 100 100 80 75 89 100 1 100 100 100 100 100 100 0
100 0
0 0
0 0
0 0
0 0
26 26 26 26 26 27 27 27 27 27 27 27.5 27 28 27.5 28 27 27.5 27.5 27.5 27 27 27 27 27 26.5 25 26 25.5 24.5 24.5 24 24 22 17 17 17 19.5 17 17 16 15 19.3 13 13 07/06/2005 07/08/2005 07/13/2005 07/15/2005 07/20/2005 07/22/2005 07/27/2005 07/28/2005 07/29/2005 08/03/2005 08/05/2005 08/09/2005 08/11/2005 08/16/2005 08/19/2005 08/24/2005 08/26/2005 08/30/2005 09/02/2005 09/06/2005 09/08/2005 09/12/2005 09/20/2005 09/22/2005 09/23/2005 09/26/2005 09/31/2005 10/04/2005 10/07/2005 10/12/2005 10/14/2005 10/18/2005 10/21/2005 10/24/2005 10/28/2005 11/01/2005 11/03/2005 11/09/2005 11/10/2005 11/15/2005 11/18/2005 11/21/2005 11/23/2005 11/28/2005 12/01/2005 677 1132 998 186 303 100 341 627 663 500 250 1067 0
464 367 237 467 267 85 115 483 105 117 250 317 917 80 214 433 131 33 52 0
817 1795 105 26 64 95 125 16 80 0
0 0
26 26 26 26 26 27 27 27 27 27 27 27.5 27 28 27.5 28 27 27.5 27.5 27.5 27 27 27 27 27 26.5 25 26 25.5 24.5 24.5 24 24 22 17 17 17 19.5 17 17 16 15 19.3 13 13 INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT lant Raw Wi lant Raw Wi lant Raw W.
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[ant Raw Wt (ant Raw WM lant Raw Wc lant Raw WM lant Raw Wc lant Raw Wc lant Raw Wi lant Raw WE lant Raw Wc lant Raw Wc lant Raw Wi QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock CFT CFT CFT Dick Adcock CFT CFC CFT CFT CFT CFT CFT CKC CKC CFT CFT Dick Adcock Dick Adcock CFT CFT CFT CFT CFT CFT Dick Adcock Dick Adcock Dick Adcock CKC DLA Dick Adcock Dick Adcock Dick Adcock CKC Dick Adcock Dick Adcock INPLANT lant Raw WE INPLANT lant Raw Wi INPLANT lant Raw WE INPLANT lant Raw Wi INPLANT lant Raw WE INPLANT lant Raw WE
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 -
-(INTEROFFICE SB-)
SODDY-DAISY TN 37384 edIt WA-SEQUOYAH NU CEAR PLANT Location HAMILTON COUNTY----------
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN00265 101 G F - FINAL PERMIT HAGENUMBERI DIFFUSER DISCHARGE Form Approved.
OMB No. 2040-0004 NG E[RIOQ I EFFLUENT IYEAR IMO DAY I Y MO I DAY From 05 l 11 l 01 l To 05_
11 1 30 ATTN: Stephanie A. Howard NO DISCHARGE [I A NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE
____E.X OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS CHLORINE, TOTAL RESIDUAL SAMPLE 0020 0.030 19 0
30/30 GRAB
.MEASUREMENT 0.2
.031 50060 1
0 0
~
~
~
.0;~~MGLWEK.CA EFFLUENT GROSS VALUE A_
MAXL D
-i; TEMPERATURE - C, RATE OF SAMPLE 0.3 62 0
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0 0
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CONTIN' CALdT EFFLUENT GROSS VALUE IE RA I
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U SAMPLE MEASUREMENT N 6"t X" Al e
SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT them NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assum that qualified personnel o
LEON DA J. Randy Douet property gather and ev-aua the Information submitted. Based on my inquiry of the person or persons who manage the system, or thoe persons directly responsile for gatheing r
Principal Environmental Engineer 423 843-6700 05 12 14 information, the Information submitted Is, to the best of my knovtedge and belief, true, Site Vice President accurate, and complete. tam anwae that theire are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and inipisonment for knowIng violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
_CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all aftachments here)
The following injections occurred: 1. H-1 5DM (max. caic. conc. was 0.036mg/L-limR 0.050mg/L) 2. H-1 5OM (low detection level analytical method was <0.020mg/L-limit 0.050mg/L)
_P For 33_- (RE 3_9 Pviu edition ma be use
.aeo 2.......
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 2 of 2
PERMITTEE NAMEIADDRESS (Include FaciiNameLocatIon if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
_ _ _jNTEROFF9CE 5S2&--
SODY - DAISY_
Th 37384 fac!y A -A SEQUOYAH NUCLEAR PLANTj Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR For DISCHARGE MONITORING REPORT (DMR)
(SUER 01)
Om I
TN026450 l101 T
F-FINAL l
PERMIT NUMBER LPl RGE NUMBER BIOMONITORING FOR OUTFALL 101 IMONITO~RING PEROIOD EFFLUENT I YEAR IAO I DAY I lYI MYEA DAY 1
A n7 n-r m Approved.
B No. 2040-0004 ATTN: Steohanle A. Howard Froml 05 I 11 I 01 I Tol 05 11 130 l 114V U101-11MMjr-NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
21180 COMPOS CERIODAPHNIA MEASUREMENT 23 TRP3B 1
0 0
PERCENT cE COMP EFFLUENT GROSS VALUE
~~~ljt.jMK 2777~IRI IC25 STATRE 7DAY CHR SAMPLE
>100.0 23 0
2/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1
0 0
PERCENT SEm m
EFFLUENT GROSS VALUE M~
~.
~
vI SAMPLE MEASUREMENT
% W zv-SAMPLE MEASUREMENT SAMPLE MEASUREMENT
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'.t NAME/TITLE PRINCIPAL EXECUTIVE OFFICER J. Randy Douet Site Vice President I Certify under penalty of law that this doC1aent and all attachments were prepared under my direction or supervision in accordanc with a system designed to assure that qlualified personnel property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the systemr or those persons directly responsible for gathering the information, the Information submitted Is, to the beat of my knowledge and belief, true, accurate, and complete. I am swie that there are significant penalties for submitting false information, including the possibility of line and Imprisonment for knowing violations.
Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTM OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference attachmentS here)
Toxicity was sampled 10/30-11/4 and 11/14-11/19. These sampling events Cover the requirement stated in the BICTP, 'Whole effluent toxicity testing (blomonitoring) of Outfall 101 shall be undertaken once per year when oxidizing biocides (Towerbrom 960) are being used and once per year when non-oxidizing biocides (H-1 50M) are being used." Reports are attached.
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E...)1 r v o s e it o s m y b s e a e 1 o EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of I
December 13, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides the results of compliance testing using fathead minnows and daphnids.
Outfall 101 samples collected October 30- November 4 and November 14-19, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Environmental Testing Solutions, Inc. performed chronic toxicity tests from November 1-8, and November 16-23, 2005. The November 1-8 toxicity tests were conducted with samples collected from SQN Outfall 101 during non-oxidizing (November 1-4) and oxidizing (October 30-November 4) biocide applications. The November 16-23 toxicity tests were conducted with samples collected from SQN Outfall 101 during oxidizing (November 14-19) biocide applications. An internal peer review of the reports was performed by TVA biologists and the Senior Toxicologist and it was determined that incorrect serial dilutions were prepared.
Previous permit dilutions of 10.98, 22.0, 43.9, 72.0, and 100% were used instead of the dilutions of 11.3, 22.6, 45.2, 72.6, and 100% specified in the recently renewed permit, effective September 1, 2005.
This incident has been assessed as a laboratory deviation, and it will be identified as such in the final reports. The EPA chronic test manual (EPA-821-R-02-013) states that deviations must be evaluated on a case-by-case basis and deviations may or may not invalidate a test result "depending on the degree of the departure and the objective of the test." TVA biologists and the Senior Toxicologist have considered the degree of the deviation in this particular case and find no potential or observed impact on the test results. Therefore the test results for the subject tests are considered valid.
Ruth Ann Hurt, SB 2A-SQN December 13, 2005 Page 2 In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment):
Files, R&TA, CTR IB-M SQN November 2005M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: December 8. 2005
- 1. Facility / Discharger: Seguovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 3266
- 7. lQ10: 2.992.4
- 8. Outfall Tested: 101
- 9. Dates Sampled: October 30-November 4, 2005
- 10.
Average Flow on Days Sampled (MGD): 1,554.4. 1,551.2. 1550.7
- 11.
Pertinent Site Conditions: H-15OM (non-oxidizing biocide) was injected from November 1-4, 2005. The dates and times for the H-150M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period.
Injection Location I Date/Start-Time (ET) l Date/Ending-Time (ET)
Raw Cooling Water (RCW) 11/01/2005-0940 11/04/2005-1200 Towerbrom 960 (oxidizing biocide) was injected from October 30-November 4, 2005.
The dates and times for the Towerbrom 960 injections are in the following table. See Appendix B for complete additional chemical application information during the sample collection period.
Injection Location Date/Start-Time (ET)
Date/Ending Time-(ET)l Essential Raw Cooling Water (ERCW) 103/05001/420540 Train A and B 10/30/2005-0000_
11/04/2005-2400l Raw Cooling Water (RCW) 11/04/2005-1345 11/04/2005-2400
- 12. Test Dates: November 1-8. 2005
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.98. 22.0.43.9. 72.0. 100.0 Intake: 100.0 Pimephales promelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: ICs5 = 45.2%
- 17. Test Results: Outfall 101: Pimephales promelas: IC2 > 100%
Ceriodaphnia dubia: IC,; > 100%
UV treated Outfall 101: Pimephales promelas: ICq
> 100%
- 18. Facility
Contact:
Sterhanie Howard Phone #: (423) 843-6713
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected October 30 - November 4, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date Sample (MM/DD/YY)/
(MM/DD/YY)/
Amval TiRtCa*
(MM/DD/YY)/
ID Time (ET)
Time (ET) emp.
Time (ET)
(OCTime (ET)
Collected Received (C)
(mpL)
Used By 101 10/31/05-0400 10/31/05 1823 1.1, l.6'
<0.10 11/02/05 1249 10/30/05-0515 to 11/01/05 1202 Intake 10/31/05-0415 10/31/05 1823 0.8
<0.10 11/02/05 1249 101 11/01/05-0700to 11/02/05 1500 2.5, 1.3t
<0.10 11/03/05 1217 11102105-0600 11/04/05 1121 Intake 11/02/05-0615 11/02/05 1500 1.5
<0.10 11/04/05 1121 11/03/05-0600 to 11/05/05 1134 101 11/04/05-0500 11/04/05 1424 1.3, l.7t
<0.10 11/06/05 1106
___11/07/05 1110 11/0305-065 to11/05/05 1134 Intake 11/04/05-0515 11/04/05 1424 1.2
<0.10 11/06/05 1106 11/0/05-51511/07/05 1110 TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 + 1.0C) in a warm water bath.
Aliguots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.
Inc.) for 2 minutes.
3
fTest Organisms:
Pimephales promelas
- Ceriodaphnia dubia
- 1. Source:
Aquatic BioSystems. Inc.
In-house Cultures
- 2. Age:
21-22.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old
<24-hours old Test Method Summary:
- 1. Test Conditions:
Static. Renewal Static. Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101 11/01/05 - 1202 ET 11/01/05 - 1221 ET 11/01/05 - II10 ET
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101 11/08/05 - 1209 ET 11/08/05 - 1229 ET 11/08/05-1119ET
- 8. TestTemperature: Outfall 101:
Mean= 24.7 0C (24.1 - 25.20C)
Test Temperature: UV-Treated Outfall 101:
I Mean = 24.9 0C (24.4 - 25.3oC) 4ean = 24.81C 24.1 -25.30C0
- 9. Physical / Chemical Measurements: Alkalinity hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville.
CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales vromelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 1-8. 2005 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - da s)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 98 98 98 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100.0%
-100 100 100 100 100 100 100 Intake 100 100 I 100 100 100 100 98 Test Solutions Mean Dry Weight (mg)
(% Effuent) jpcate number) 1 2
3 4
Mean Control 0.582 0.669 0.718 0.683 0.663 10.98%
0.676 0.621 0.737 0.671 0.676 22.0%
0.591 0.641 0.646 0.650 0.632 43.9%
0.679 0.629 0.711 0.634 0.663 72.0%
0.582 0.718 0.581 0.682 0.641 100.0%
0.712 0.691 0.609 0.621 0.658 Intake 0.592 0.656 0.606 0.697 0.638 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 1 00/LC 5o: TUc = 100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 1-8. 2005 using effluent from Outfall 101.
Percent Surviving Test (time interval use - days)
Solution 1
2 3
4 5
6 7
Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 f00 100 100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) s Efluent)Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 32 28 26 25 31 24 30 26 26 29 27.7 10.98%
27 30 27 29 28 30 30 27 30 26 28.4 22.0%
29 30 31 31 28 30 31 33 29 28 30.0 43.9%
33 30 31 28 32 32 34 30 32 29 31.1 72.0%
36 32 30 33 31 30 31 36 33 34 32.6 100.0%
35 32 33 27 33 32 32 34 32 36 32.6 IC25 Value: > 100%
Calculated TU Estimates: <1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 1 00/LC 5o: TUc = 100/ IC25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 1-8. 2005 using water from Intake Percent Surviving Test (time interval used-days)
Solutions2 3
4 67
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
Data (replicate number)
( Effluent) 1 2
3 4
5 6
7 8
9 l 0
Mean Control 26 34 27 31 29 29 29 27 29 30 29.1 Intake 36 32 31 35 29 35 33 34 34 37 33.6 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permnit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/ IC25 7
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 1-8. 2005 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 98 72.0%
100 100 100 100 100 100
.100.
100.0%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)
Test Solutionsreplicate number)
(%Effluent) 1 2
3 4
Mean Control 0.625 0.680
.0.637 0.657 0.650 10.98%
0.690 0.719 0.676 0.692 0.694 22.0%
0.592 0.649 0.640 0.667 0.637 43.9%
0.658 0.594 0.677 0.671 0.650 72.0%
0.573 0.666 0.669 0.652 0.640 100.0%
0.645 0.662 0.640 0.650 0.649 Intake 0.664 0.686 0.674 0.685 0.677 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (OC 25)
. Pimephales promelas November 1-8, 2005 1238 7-days KCl 0.60 gfL Ceriodaphnia dubia November 1-8, 2005 1101 7-days NaCI 1.08 g/L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pirnephales prornelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, November 1-8, 2005.
Test Sample ID Temper re Dissolved Oxygn (mg/L) pH(
U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pimhos/em) (mgIL CaCO3) (mg/L CaCO 3) Chlorine (mgIL)
Control 24.8 24.5 7.6 7.3 7.77 7.59 312 58 92 24.6 - 24.9 24.2
- 24.8 7.4 7.9 7.0 7.8 7.53 8.10 7.31 -
8.03 306 315 58 59 85 97 10.98%
24.8 24.5 7.8 7.2 7.83 7.53 295 24.6
- 25.0 24.2
- 24.7 7.4 8.1 6.9 7.8 7.56 8.14 7.28 -
7.98 288 298 A2.0%
24.8 24.4 7.8 7.2 7.80 7.54 283 z20%
24.6
- 25.0 24.2 24.6 7.4 8.1 6.8 7.8 7.56-8.14 7.29 - 8.02 279 292 I
49%
24.8 24.5 7.9 7.3 7.78 7.55 255 246-25.1 24.1 24.7 7.5
- 8.
- 6.
-7.8 7.54
.4 7.30 - 8.04 247 6
C"24.9 24.4 7.9 7.3 7.76 7.56 218 7.% 24.6
- 25.!
24.3 24.7 7.6 8.3 6.8 7.9 7.50 8.14 7.28 - 8.05 212 225 100.0%.
24.7 24.5 7.8 7.3 7.74 7.57 183 64 74
< 0.10 247-25.2 24.2 24.7 7.6 -
8.3 6.8 8.0 7.47-8.16 7.29 -
8.06 177 191 62 67 63 85
<0.10 -
<0.10 Intake 25.0 24.5 7.8 7.3 7.72 7.57 181 63 74
<0.l0
____________24.8 25.!
24.1 24.7 7.5 8.3 7.0 7.8 7.44 -
8.16 7.31 8.10 177 f86 61 66 73 75
<0.10 -
<0.10 Control 24.6 25.0 17.6 7.6 7.77 7.72 312 58 92 246-24.9 24.7 -
25.3 7.4 7.9 7.4 7.9 7.53 8.l0 7.45 8.07 306 315 58 59 85 97 10.98%
24.4 24.9 7.8 7.6 7.83 7.73 295 24___
4__ - 25.1 24.6
- 25.3 7.4 8.1 74 7.9 7.56 8.14 7.49 8.09 288
-298 22.0%
24.8 25.0 7.8 7.7 7.80 7.72 283 24.5 25.1 24.7
- 25.3 7.4 8.1 7.4 8.0 7.56 -
8.14 7.50 8.10 279 292 24.9 24.9 7.9 7.7 7.78 7.73 255-
_____24.5 25.1 24.7
- 25.2
-7.5 -
8.3 7.5 8.0 7.54 8.14 7.51 8.11 247 264 24.9 25.0 7.9 7.7 7.76 7.71 218--
7.% 24.6 25.1 24.7 25.2 7.6 8.3 7.5 7.9 75 8.4 7.50 -
8.11 212 225 10.%
24.9 24.9 7.8 7.6 7.47.70 183 64 74
< 0.10 100.0%_
24.6 - 25.2 2.6 -
25.3 7.6 8.3 7.4 7.9 7.47 -
8.16 7.47 - 8.11 177 191 62 67 63 85
<0.10 -
<0.10 Intake 24.8 24.9 7.8 7.7
.47.728 87.69 181 63 74
< 0.10 24.5 25.0 24.8 25.2 7.5 8.3 7.4-7.9 7.44 - 816 7.48 8.14 177 186 61 66 73 75
<0.10 -
<0.10 Overall temperature (C)
Pimiephalespromelas Ceriodaphnia dubia Average 24.7 24.9 Minimum 24.1 24.4 Maximum 25.2 25.3 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chenlstry ean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, November 1-8, 2005.
Test Sample ID Temperature (C)
Dissolved Ox gen (mg/L)
H (S.U.)
Conductance Initial Final Initial Final Initial Final (Itmhos/cm)
Control 24.9 24.5 7.8 7.4 7.71 7.56 300 24.7 - 25.0 24.1 - 24.8 7.2 -
8.3 6.8 -
8.0 7.57 -
8.11 7.31 - 8.03 290 -
312 10.98%
25.0 24.5 7.8 7.3 7.70 7.56 295 r
24.8 - 25.1 24.2 - 24.8 7.3 8.3 6.9 -
7.9 7.50 - 8.13 7.30 - 8.04 287 -
307 25.0 24.6 7.8 7.3 7.70 7.55 284 24.8 - 25.1 24.4 - 24.7 7.4 -
8.3 6.9 7.7 7.50 - 8.12 7.30 - 7.99 275 -
292 43.9° 25.0 24.4 7.9 7.3 7.69 7.56 256 24.8 - 25.2 24.1 - 24.7 7.4 8.3 6.9 7.8 7.49 - 8.13 7.30 - 8.04 252 -
261 72.0%
25.1 24.5 7.8 7.4 7.68 7.60 221
__24.8
- 25.2 24.2 - 24.6 7.4 8.2 7.0 7.8 7.49 - 8.14 7.29 - 8.06 216 - 226 100.0%
25.1 24.5 7.9 7.3 7.67 7.58 185 24.9 - 25.3 24.1 - 24.6 7.5 8.3 7.0 -
7.8 7.48 - 8.14 7.27 - 8.09 181 193 Intake 25.1 24.4 7.9 7.3 7.66 7.57 184 24.9 - 25.2 24.1 - 24.6 7.6 8.3 7.0 -
7.7 7.43 8.18 7.27 - 8.06 173 -
198 Overall temperature (C)
Pimephales promelas Average 24.7 Minimum 24.1 Maximum 25.3 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected October 30-November 4, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC2s values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas & 2. Ceriodaphnia dubia Environmental Testing Solutions, Inc. performed chronic toxicity tests from November 1-8, 2005. The toxicity tests were conducted with samples collected from SQN Outfall 101 during non-oxidizing (November 1-4) and oxidizing (October 30-November 4) biocide applications. An internal peer review of the reports was performed by TVA biologists and the Senior Toxicologist and it was determined that incorrect serial dilutions were prepared. Previous permit dilutions of 10.98, 22.0,43.9, 72.0, and 100% were used instead of the dilutions of 11.3, 22.6, 45.2, 72.6, and 100% specified in the recently renewed permit, effective September 1, 2005 The dilutions that were used in the tests were virtually the same as what the current permit requires (' 1.3% effluent difference) given the variability inherent in performing whole effluent toxicity tests. Additionally, the error is judged to be inconsequential relative to evaluation of the test results since no toxicity to either species (Pimephales promelas or Ceriodaphnia dubia) was observed (i.e., IC25 > 100% effluent) during either test period.
A process change to TVA's NPDES biomonitoring compliance program has been implemented whereby the contract laboratory must confirm via email to TVA the exact dates that toxicity tests will be performed and the dilution series that will be used, prior to conducting all future tests.
12
This incident has been assessed as a laboratory deviation, and it will be identified as such in the final reports. The EPA chronic test manual (EPA-821-R-02-013) states that deviations must be evaluated on a case-by-case basis and deviations may or may not invalidate a test result "depending on the degree of the departure and the objective of the test." TVA biologists and the Senior Toxicologist have considered the degree of the deviation in this particular case and find no potential or observed impact on the test results. Therefore the test results for the subject tests are considered valid.
DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephales promelas None
- 2. Ceriodaphnia dubia None PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by EPA Method 170.1.
- 5. The pH was measured by EPA Method 150.1.
- 6. Conductance was measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
13
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited-to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCl.
- 2.
Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nuclear Plant Biomonitoring November 1-8, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling
Table B-l. Sequoyah NuclearPlant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 4, 2005 Sodium Towerbrom PCL-222 PCL01 CL363 Cuprostat-CL3 Dae Hypochlorite'-
PFgH-13 Dae7 mg/L mg/L~`,J mg/L mg/L'.
~
m/L DeTT.g TRC Phosa Copolymer DMAD" mgua TRC -
-Azole u
03/12/1998 0.016 -
03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998
<0.001 0.044 0.015 09/13/1998
< 0.001 0.044 <
0.015 09/14/1998 0.008 '
0.044 0.015 02/22/1999
< 0.001 02/23/1999 0.005 02/24/1999 0.009 '.
02/25/1999 0.012 '
02/26/1999 0.008 02/27/1999
<0.001 02/28/1999
< 0.001
-i t
08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012
-0.068 0.024 08/20/1999 0.023 0.070, ;I,`
0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 9 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.006 08/24/1999 0.016 0.067 0.023 0.020 2
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 4, 2005 Sodium Cuprostat-D Towerbrom PCL-222.1 PCL-401 CL-363 PF H-130M HDoateite mg/L m/
mg/L mg/L..;~
e Dae TRC Phosphate Copolymer DMAD-Qtit V
-~
A zo le 01/31/2000
<0.002 0.026 0.009 02/01/2000 0.011 0.026 I4E 0.028 02/02/2000 0.028 0.026 K; 0.009 0.006 ;
02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000
< 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000
< 0.0057 0.055-:
0.019 07/27/2000 0.019 0.055 '
0.019 07/28/2000 0.0088 0.053 '.-
0.018 0.004 0.108 07/29/2000
<0.0088 0.055 0.019 07/30/2000
< 0.0076 0.055 0.019 07/31/2000
< 0.0152 0.055 0.019 0.006 ;
08/01/2000
< 0.9141 0.055 0.019 0.005.
12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.005
^
12/13/2000
<0.0120 0.025-0.020 12/14/2000
< 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 -
0.020 0.005 12/16/2000
< 0.0036 0.025 0.020 12/17/2000 1
<0.0036 0.025.
1 0.020 08/26/2001 0.017 0.06 0.021 0.006,
08/27/2001
<0.0096 0.06 0 021 0.005-0.021 'r 08/28/2001
<0.0085 0.06 0.021 d
08/29/2001
<0.0094 0.059 0.020 0.005 0.021' 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001
<0.005 0.059 0.020 4
11/25/2001
<0.0044 11/26/2001
<0.01 19 0.024 0.02 0.005 l 11/27/2001
^
0.0137 0.023 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001
<0.0043 0.024 0.02 12/09/200 1
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 l-12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001
<0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 3
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 4, 2005 Sodium Cpott Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M Date Hypochorite,
mg mg/PF mg/1 mg/.
PF mg/IPe mWL TRC Phosphate Copolymer DMAD '
m/L T C
_ _A zoleQ u t 01/02/2002
< 0.0079 0.023 0.02 0.006 01/03/2002
< 0.0042 0.023 0.014 01/04/2002 0.0124 0.024 0.014 0.009.
01/05/2002
<0.0042 01/06/2002
< 0.0042 01/07/2002
< 0.0089 0.024 0.014 0.006 02/24/2002 0.004-02/25/2002
< 0.004 0.023 0.023 02/26/2002 0.0143 0.023 0.023 0.007 02/27/2002
< 0.0041 0.023 0.023 02/28/2002
<0.0041 0.024 0.008 03/01/20021
<0.0041 0.024 0.008 05/05/2002 05/06/2002 0.058 0.02 0.014' 05/07/2002 0.058 0.02 0.015 05/08/2002 0.056 0.019 05/09/2002 0.057.
0.02 0.014 05/10/2002 1 0.056 0.019 08/04/2002
<0.0058 08/05/2002
<0.0058 0.053, 0.018 A,
0.025.,,
08/06/2002 0.0092 0.053 0.018 08/07/2002
<0.0107 0.055-'
0.019 0.007 '
08/08/2002
<0.0061 0.055 0.019 08/09/2002 1 0.0152 0.054 0.018 0.008 -
10/06/2002
<0.00497 10/07/2002 0.0153 0.054 0.018 0.009 10/08/2002
<0.0092 0.054 0.018 0.007 10/09/2002 0.0124 0.053.
0.018 0.009 10/10/2002 0.0134 0.054 0.018 0.009 10/11/20021
<0.0042 0.054 0.018 01/12/2003
<0.0035 I
01/13/2003
<0.006 0.025' 0.019 0.009 01/14/2003
<0.0118 0.026, 0.020 01/15/2003
<0.0063 0.026 0.020 0.009 01/16/2003
<0.0034 0.026, 0.020 01/17/2003
<0.0034 0.026 0.009 04/06/2003
<0.0073 04/07/2003
<0.0189 0.021 04/08/2003
<0.0117 0.021 04/09/2003
<0.0139 0.021 0.016 04/10/2003
<0.01 13 0.021 0.018 04/11/20031
<0.0073 1
0.022 4
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 4, 2005 Sodium:;
Curstt De pochloite Towerbrom PCL-222 PCL-401 CL-363, Cuprostat-H-130M Date mfL m/L'mg/L.,s mgI.
mg/L DMAD mg/Lg/
Date
' mc' TRC e
Copolymer M
mzol 06/15/2003
< 0.0045 06/16/2003
< 0.0037 0.057 0.020 0.022 06/17/2003
< 0.0048 0.041 0.014 0.024, 06/18/2003
< 0.0048 0.041 '
0.014 0.024 06/19/2003
< 0.0085 0.058 0.020 0.025 '
06/20/2003
< 0.0048 0.058 0.020 0.025 08/03/2003
<0.0050 08/04/2003
<0.0050 0.058 0.020 08/05/2003
<0.0051 0.057 0.020 0.025 08/06/2003
<0.0084 0.057 0.020 0.025' 08/07/2003 0.0129 0.057 0.020 0.024 08/08/20031 0.0153 0.057',-
0.020 0.009 10/05/2003
<0.0043 0.057 0.020 10/06/2003
<0.0043 0.057 0.020 0.025 10/07/2003
<0.0090 0.057 0.020 0.025' 10/08/2003
<0.0106 0.057 0.020 0.025' 10/09/2003 0.0181 0.026 ','--
0.022 0.025 10/10/2003 0.0183 0.026.,
0.024 0.009 02/01/2004 0.0093 0.027k.
0.009 02/02/2004
<0.0034 0.026 0.009 02/03/2004
<0.0034 0.026 0.009 02/04/2004 0.0124 0.026, -£-
0.009 0.009 02/05/2004
<0.0034 0.0266 0.009 02/06/2004 0.0105 002 0.009 0.010' 05/04/2004
<0.0123 0.026_',,
0.019 0.025 05/05/2004
<0.0144 0.026'1:,
0.014 0.009 0.025 05/06/2004
<0.0146 0.037 0.013 0.025 05/07/2004 0.0227 0.058 t
0.020 0.009 0.025,'
05/08/2004 0.016 0.060 0.021 05/09/2004
<0.0104 0.058 0.020 07/04/2004 0.0217 0.057 0.019 07/05/2004
<0.0085 0.057 0.020 0.009 07/06/2004
<0.0077 0.0588 0.020 0.031 07/07/2004 0.0252 0.056 0.019 0.031 '
07/08/2004 0.0223 0.057 0.019 0.009 07/09/2004 0.0182 0.057 0.020 0.009 5
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 4, 2005 SodiumNalco Hypoclit Towerbrom PCL-222.
PCL-401 CL-363 Cuprostat-PF H-130M H-150 73551 Date e
mg/L mgfL mg/L mg/L mg/L mg/LA5 mg!L mg/L't TRC Phosphate Copolymer DMAD Azole Qu EO/PO Qu 11/07/2004
<0.0187 0.000.,.
0.014 11/08/2004
<0.0192 0.047 0.030 11/09/2004
<0.0233 0.048 0.016 0.041:
11/10/2004
<0.0149 0.047 0.016 0.041-11/11/2004
<0.0149 0.049 0.017 0.043 11/12/2004
<0.0253 0.048 0.017 0.042 02/06/2005
<0.0042 0.028 0.010 02/07/2005
<0.0116 0.028'
-0.010 0.007 02/08/2005
<0.0080 0.028 0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005
<0.0042 0.028 0.010 02/11/2005 0.0155 0.028 0.010 0.007 06/05/2005 0.0063 06/06/2005 0.0043 0.037 06/07/2005 0.0103 0.037 06/08/2005 0.0295 0.037 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 0.009 07/18/2005 0.0150 0.026 0.009 0.036 07/19/2005 0.0163 0.026 0.009 0.036 07/20/2005 0.0209 0.026 0.009 0.014 0.036 07/21/2005 0.0242 0.026 -
0.009 07/22/2005 0.0238 0.054 0.018 0.014 10/30/2005 0.0068 10/31/2005 0.01 12 11/01/2005 0.0104 0.035 11/02/2005 0.0104 0.036 11/03/2005 0.0117 0.036 11/04/2005 0.0 165 0.035 6
Sequoyah Nuclear Plant Biomonitoring November 1-8, 2005 q Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD 4
Page of 1 1-I Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Other (specify): Express Courier Client General Comments:
Facility Sampled: Sequoyah NP NPDES Number: TN0026450 if iii i
I i
Collected By: Duane Brigman Fax:
828-350-9368 ave,:i touswsA'~
Field Identification/
GrabComp.
Colletion DathTime Container Flow Sample Description Number &
MOD Rain Event?
Volume (Mark as Appropiate)
Collected Date lme f
Yes s,
No Trace Rm
.. ~~~
Iniches
'h.be SQN-101-TOX Comp 10/30/05-10/31/05 2 (2.5 gal)
NA i i
. /$q.4 o co",
w~oo SQN-INT-TOX Comp 10/30/05-10131/05 1 (2.5 gal)
NA l
Sample Custody - Fill In From Top Down 4
A d
Relinquished By (Signature):
DaterTime Received By (Signature):
Date ye Duane Brigman 10/31/05 r3qL{
Express Courier 10/31/05 134 t Express Courier,)10/31/05 ESX ionr 10/31/5 1IZ.3A Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
I
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1
of -1
- ]
I I
Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number TN0026450 Collected By: Duane Brigman Clte4 O-uAKSS Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 T1liv;-A nv ftfrel. flnr.-
FedEx Other (I General
- 4 AJ7 A*
- JfllfJ.
UPS Bus Client pecify): Express Courier C
Comments:I Fax:
828-350-9368 Field Identification /
Grab/Comp Collection Date/ime Container Flow Sample Description Number &
(MGD)
Rain Event?
x x.
Volume (Mark as Appropriate) M
____Collected Rdinquishe Day (gte Da.
Ifime No TBae (Siga,
.ture):..
.ae_,_
_inches SQN-0-TOX Comp 11/1/05-1112105 920 2 (2.5gal)
NA 1I/i2/
400 SQN-INT-TOX Comp 11/1/105-11/2/05 1 (2.5gal)
NA
_______________Sample Custedy - Fill In From Top Down 4
AA-Relinquished By (Signature):
DaterTime Received By (Signature):
a rine Duane Brigman 11/2/2005 Expre 11/2/2005 Express Courier lb-DU 11/2/2005 lTS 4
CjJ.4-_
11/2005 15(x 5c,
l
-II I
A.
A.
Instructions: Clients should fill in all areas except those in the "laboratory Use" block. Biomonitoring samples are preserved by storing them at 6-C and shipping them in ice. The hold timel for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
I
BIOMONITORING CHAIN OF CUSTODY RECORDPae1o1 Page _1_ of _i_
I w
U -
IClient: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number TN0026450 Environmental Testing Solution, Inc.
3 5 1 Depot Street Ashville, NC 28801 Phone:
828-350-9364 FedExr UPS Bus Client Delivered By (Circle One):
Other (specify): Express Courier General Comments:
Collected By:- Duane Brigman Fax:
828-350-9368 Field Identification/ IGrab/Comp, Collection Datefrime Container Flow Sample Description Number &
(MOD)
Rain Event?
trtcye volume (Mark as Appropriate)
Collected Date Ti mmgmz &,Ya if Yes.
No Trame~$~
iw Tm'p y
Tws-A4'X SQN-101-TOX Comp 11/3/05-11/4/05 2 (2.5gal)
NA 7
Q QL -
M O_
SQN-INT-TOX Comp 11/3/05-11l/4/05
~
1(2.5 gal)
NA.*:
________________Sample Custody - Fill In From Top Down Cu.4
.~cadi d
Relinquished By (Signature):
Date/Time Received By (Signature):
Da me Du eB imn11/4/05 E
r Cure 11/4/05 Express Courier 9 i,1 1/4/05
,4zq ETS 11/4/05 instructions.: Csernts sholUI 1111 in all areas except tliose in tie Lauboratory Use-block. 1ioiomomtormig samples are preserved by storing them at 6'C and shipping them in Ice. Lnc 11ow time lor each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samiples with ample time to initiate testing within that time frame.
Samples shipped ovemnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
En ronmental Testing Solutions, Inc.
Chronic Whole Effluent F
St Page I of 6 Toxicity Test (EPA-821-R-02-013 Method 1000.0) pecies: Pimephalespromelas Client: TVA Facility: Seguovah Nuclear Plant NPDES #: TN 0026450 Project #: 2 11 County: Hamilton Treatment: Non-treated Outfall: 101 Dilution reparation information:
Comments:
Dilution prep (%)
I0.98 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism Information:
Test Information:
Organism age:
Z2l -Mt
- 22. S tcksS Ocb Randomizing template:
%I Lo Date and times organisms
- l. bso -rS 1
to Incubator number were born between:
Organism source:
A6C eI ep 10 35-oS Artemia lot number.
J* j1 Transfer bowl information:
pH =
Temperature G
°C Total drying time:
5, 2t.0 Date /Time in: l-ot -oS 1?.SS Average transfer volume:
tO Date / Time out: tl6Ci.-65 172 l Oven temperature:
oV C Dailyfeeding and renewal Information:
Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time K
U 0
ix-CA-oS IS60 120-.
It4c4-Z s
OSIOBl.ol do eO fl-az-Os os3 i's5 0 IZu%
0o-zq.oS6 OlO310 S.Olm 2
O 2
I -OS A1.5 1Sz(c 1211 lo -t-o A oS IJO2-. 17*. 13 3
1 I-cq-oc owss zs>
l
\\
l-O IS OS II~ oz. 17-t13 4
I %s-cs 084S JqSC 1 3'4
- 1-3-oS A os j4. 0%
a&-
1 \\
iI5i Om-ag. tl 11L..
0?-
6
)Is-a>o o&FS 1,A.S7 11 IC)
IIos-cSs oSIX& tvio 01%L0 7-I Control Information:
Acceptance cnteria Summary of test endpoints:
% Mortality:
l g7.
s 20%
7-day LCs 0 j lC67%
Average weight per initial larvae:
O.b3!2; NOEC Average weight per surviving larvae:
O. (o"3 2 0.2s mg/larvae LOEC
>Ioot ChV
> I lcb 1c25 I >It0%.
l
- Environmental Testing Solutions, Inc.
I
~Species: Pimephalespromelaq Client: TVA/I Sequoyah Nuclea Page 2 of 6 r Plant - Non-treated Date: _A-o0-OS:
Survival and Growth Data I
I I
Day CONTROL 10.98%
l 22%
A B
C D
E F
G H
I l
K L
0
_D 1to0' 0 IC t/0o t
10 10 /0 10
/0 to /0,I0 0
//0 lo lo /0 /0 /0 2/0 J
0
/D I C)D 10 0o
/0
°0
/0 /0 10 4'
,o) lo 10 I Dc o1
/0 10 to/O ID
!__ /_
Z0
/0
/0 10
/0/0 0
/0 1o IC )o /0 s_
/0
/0
/0
/0
/0
/0
.d
/0
/0
/0 6
._IO____
ID 1010 10 1
10 I10 C) I0 10 l
0 IC)
It 10
> ().
/D I
a o
o
- A - Pan weight (mg)
Color ldentdfication:mlo
.4;:
1+7 H.1c'116 io t i'w jq.jj,q.:7l 11.0 2
!')
~0z. 41 Analyst: #&-Vgg/1nn B - Pan + Larvae weight (Lma Zlvae 21 mS a ZZ-A-20 B0 tZ I.-Zi 20 4 10 21 tl
.01%
Lavatweiwght (mg) -A - B_
Weight per Initial number oflarvae (mg)
- C / Initial number of larvae 4'b
%0
'S
'S ao b'
I.
%0&
Or O'-
N O7
'U
%0 O7 4,
Average Percent weight per reduction Initial trom control oj, bb number of (1/%)
larvae (mg) l b -
2.z - 7 32* -
4 -1 1-I..I.
Comment codes: c - clear, d - dead, fg - fungus, k - killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r - decanted and returned, w - wounded.
Calculations and data reviewedL...
Comments:
Environmental Testine Solutions. Inc.
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
11-O1-06 Survival and Growth Data Day 43.9%
72%
100%
M N
O P
lQ R
S T
U V
W x
0 lo J/ D 1o /
_ i/
0 t
lO /0 loo /
2 lOi O O
10 I010
/0
/0
/0
/0
/0 10 I O I C l
I C) IO t O I/
/0 IC) 100
_4 ID 10
/0 C) 10 JQ > 10 t0
_6 10 /0 5
/0 10 10 /0
(-/0/
/0
/0 10 /0 6
I0 10t 1
/01010 _0 I
/0 I
t 1O (0
/0IC 11
/'
/0 10 (0
/t o
I 10 At Pan weight (mg)
Color Identicatnlg 1q,2,4 a1.'t
? 1'£.31 Ji34 I'L.1*
gqYA l1.7 iq.q&
N.
15.iS 65 Analyst:
na C
MIL:
51 e
B - Pan + Larvae weight Analyst A za iy st:
4 L~
Z 1.84
- ~.Z b.5 2 o.5$
a os zo Wet Z O.'4? Z ~l t 1SS 22..t L t.bi AZ L'
2 2 (.
Larvae weight (mg) - A - B Weight per initial number of larvae (mg)
C/ Initial number oflar O
O V
4
'O Clv
-oV Average Percent weight per reduction
- 0. t a3.7e O.
So
&.1ft Initial from control wi 317 9
number ot
(%)
l a r va e Cm )
Comment codes: c - clear, d dead, fg = fungus, k 8 killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Environmental Testine Solutions, Inc.
Page 4 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date:
t1-cI-01S Survival and Growth Data Day 100% Intake Y_
Z AA BB 10
/0
'0
/0 1
/0 1
/O 10 2
It 101 o 1)
_/C10
/o
/0 ID 10o
/O /O to 5
(
0 l10 6
O 10 10 7
tl o n to A - Pan weight (mg).
Color Iden tifcatiantrMJed.W, Analyst:- J~j=#a 04)-q M
H B - Pan + Larvae weight (mg)2 1SM ZLI Analyst:
dl 21.3S Ij.'-t 2
28 Larvae weight (mg)
A - B Weight per Initial number of larvae (mg)
O larvao
-C / Initial number oflarvae O
Average Percent weight per reduction Initial from control 0 (3
- 3. 8 c number of (M) larvae m Comment codes: c =clear, d = dead, fg = fungus, k - killed, m = missing, sk sick, sm unusually small, Ig - unusually large, d&r - decanted and returned, w = wounded.
Calculations and data revicwed:A Comments:
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Chronic Whole Effluent Toxicity Test (EPA421-R-02-013, Method 1000.0)
Species: Pimephalespremelas Quality Control Verifieatlen of Data Entry, Calculations, and Statistical Analyses Revetwed br.
Prejue member.
2173 C a e e
n uN o t f o r C i e p l i c A kw 1n te e n ty Q C leae
- W,j 400 A-A orP--i (we) umrofbaWO (a) wwuhren.
cn_
N%)
A 10 14.77 20.59 5.t2 0.532 0.5t2 Ceu C
10 10 14.55 21.24 6.69 06Q69 0.669 10.t..1 W.
Net pplbh 10 10 14.46 21.64 7.13 0.718 D
10 10 14.7S 21.5t 6.33
.6 0.683_
0 6
t3 10 10 t16.01 22.77 6.76 0.676 0.676 T10 10 14.683 20.39 6.21 0.621
.76.00.
621 i00.3 0.66 7.0 4
(1oG 0
10 14.77 22.14 7.37 0.737 0.737 an_
0 10 14.71 21.42 6.71 0.671 0.671 1
10 9
25.01 20T.92 5.91 0.657 0.591 22%
K 14.29 1
20.70 6.41 0.641 0.641 n.632
- 4.
4.7 K
10 10 15.02 21.43 6.46 0.646 0.646I L
10 10 14.42 20.92 6.50 0.650 0.650 M
10 10 15.05 21.34 6.79 0.679 0.679 4.%
N 0
1 14.27 20.56 6.29 0.629 0.663 5.9 0.629 100.0
.63 5.9 0.0 0
10 10 14.47 21.53 7.11 0.711 0.711 TP 1
10 14.3 20.65 6.34 0.634 0.634 10 10 43106 5.32 0.532 0.582 72%
R o
10 14.36 22.04 7.131 0.718 0.641 10.9 100.3 0.641 10.9 34.
a0 10 14.61 20.42 5.31 0.5-1 0.59.
to________
Z 10 10 14173 21.55 6.32 0.632 0.654 5,7 0.632 n s
_a_
_.s 10 10 14.98 22.10 7.12 0.712 0.712 10%
V 10 10 14.75 21.66 6.91 0.691 063770.691
- 10.
0.37707 lon W
10 10 35.15 21.24 6.09 0.609 06l770.609 lc
.5 X
10 10 35.35 22.06 6.21 0.621 0.621 Y
10 9
14.34 20.26 1
5.92 0.653 0.592 1 %Itae Z
30 10 14.7 21.35 6.6065
.5 5.7 0.656 97.5 0.633 AA 30 10 13.93 1.9 606 1
0.606 0.606 to___
_ __ _1 10 14.43 21.45 6.97 0.697 0.697 outrsn lof:
40.062 13.0 bmD -
MinaIum spi"t Difmmoce PSD -
Pmat MiummuSiaificnt Differane PMSD m *nm ufoftest The PMSD m the adukump l dffnt the ontrolendtnt that tebe de d ty jfma ahoe eluttomn On awoka, auigaitfit diffewunc. asee fae FaBnvm untal TedtngSooutionas In chrnic u
toxicity teto when a toxicmt reduesepeephelae Goath by 13.2% fienth conttol (dotmni thbrous refieace toxiant tog Lw PMSDbound dmied by USPA(IOth peamte) - 9.4%
Uper PMSD bound dectkeed by USHPA (9PM prcentile) - 35%
Th low end upper bondywe caled by the USEPAusIg 205 te cc ed Sitn 19 Iboratoiesf orflmpide owth as micnfece toaxnt ttets.
ed letsky Duet's MtD valo:
0.0731 pMsD:
11.0 USEPA 2000. tdentandug Ad 4Accugfr Method Viabuity Whole leut Toxiciy Alation tderth National Pollut to DischaeliuintiPopanm PA.333-Ro003. US Banvkom lProtio Agc, Cyt t Cl.L
-i TVA / Sequoyah Nuclear Plant, Outfail 101
, Non-treated_
November 01-08, 2005 Statistical Analyses LarvWlPIk Crwsw and SwrvlvalTwO. DayGrowde SttDae:
Il/D200 Test D PpFRCFR Smpl ED:
TVAI SQN Outhn 101, Noea-tated Ehd Doc IIN2D5 LAbbD IMl1-Kii*. TaftkgS5 SampSlOeLr D7-Deid-e M rgRepoit Sampe NW.
PWCHRELmA-421-R2.013 Tt %Mck
?PP.maphs promehi Comment:
CMae%
1 2
1 6
D-Coetio 0.5t20 0.6690 0.710 0.6t30 20.93 0760 0.6210 0.7370 0.6710 22 OS910 0.6410 06460 0.6500 43.9 0.670 0.6290 0.7110 06340 72 0.5*20 M7130 03810 0.6A20 100 0.7120 0.910 0.6090 0.6210 Tmoxforw: UaIhbsaarsa d l-TaSled etalk CMc-%
Mae.
N.Mea mo1 min Mal CV%
14 3Sta m
mD Mg" N-Ma D-Control 0.6630 1.OWO 0.6630 03320 C71t0 3.71t 4
0.6696 1.000D l0.93 Q6763 I.02D0 0.6763 0.6210 0370 7.025 4
4.370 2.410 O0O062 0.6696 I.000 22 0.6320 0.9532 0.6320 OIO 0.6500 4364 4
0.M66 2.410 C0OS62 0.64S6 0965
- 43.
0.6633 1.0004 06633 0.620 0.7110 5.76 4
-. 007 2410 0.0362
.646 096"s 72 0.640 09664 0.640t 0.3320 0.7130 102921 4
0.622 2410 0.062 0.6486 0.36r5 200 0.6S3 0992 0.65U3 0.6090 0.7120 7.734 4
0.133 2.410 U0062 0.6436 965 AaxiuaTat%
Sftl tk Ot" skew Yart Shtapho-WIM Tedi normaldistributio (p > 0.01) 0O95S55725 0.34 4.09465S9
- 1.OD1503 Bats Tate bdiren qul virne (-
0.7t) 2.41727773 15.0t627224 11ypethashTot(1.at0,85)
NOEC LOW CkV TV M150.
KIDP KMS AMf 41t.rb dl Demnc Test 100
>100 1
0.0623M6t 0.130073435 Q001059367 QOD2560944 0.Q330639 5,11 Tmatmnt vs D-Control LMeer pelalakds (200 Rasoples)
Pblt SD ff% amxp)
Skew IC05
>200 IC10
>100 ICIS
>100 1.0 IC20
>100 09 IC2S
>100 1C40
>100 ICSO
>100 0.7 0.6 02 0.3 0.2 0.1 0.0 0
.20 40 60 60 100 120 Dae R pobse2et 0.7 106
.0.3 CD 0.4 I-0.3 02 0.1 o 14an, 0.Kr Mne efsinchoe 04I I
rem sqvl-OI-O15data
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 01-08, 2005 Statistcal Analyses Larval Iish Grwd aad Ssrva Tout D" Ga Stut Date IUI0 Tat D.
PpFFlR Smple D.
TVA/ SQN Whkk Nana-sed EndData:
IW2D5 Lab ID.
lS-v Taftl SOL Sampeype:
DMR-Dbehargc MohgrpRAPOet SampkDater Protocol FWCHR-HPAU1-R42013 Ted Specks:
PPkUehzks prmel CMC.%
I 2
3 4
D-Col 0.520 0.6690 0.7110 0.6130 IOD 0.5920 0.6560 0.6060 0.6970 Trsmxf" m: UmtssOur=d 1-T d
Jsetek nae-%
MMA N-Me
?Mda Min Ma CV%
N MEbt CH" MSD Mas N
-Man
-Caotrol 0.6630 1.OOX 0.6630 0.0 0.7180 8.711 4
0.6630 1.000 1OD 0.637n 0.9619 6378 0.590 0.6970 7.544 4
0.672 1543 U0731 0.637:
0.9619 ARXIIurexts Stk stlc Q1itica Stew KAct Sbapko-WikaTatiuticat as adk ui
,(p>
0.01) 05942614555 0.749
.0,3112941 0.370079 P-Tt kiditta qdvarnc=s - 0.77) 1.43104506 47.46722794 1lyplsak Tstc(1AW1.0.)
MsDs MSDp MSm ME F1-Prb C
HotMOuductTestkilstesnoaksni=ntdiffaenca 0.07306709 0.110206772 0.001275125 0.00227792 0.526889394 1,6 nstmbnb vs D4nmoI Lksur ltarpalato (200 Rounmpla)
PWt SD fs% C4EZP)
Skew 1003
>100 IC10
>100 IC15
>too 1.0 IC20
>100 OS IC25
>1 0.
IC0
>100 0.° CSO
>100 0.7 05 IOA 0.3 0.2 0.1 0.0 0
20 40 so so 100 120 DOse %
Dae-Lsapote WM Cs2 0.7 0.6 90.3 0.2 0.1 I
I-tan. 0.05 lAI of ssrtgnlcaee i
g sgwlj-01-05data
Environmental Testingy Solutions. Inc.
Page I of 7 Environmental Testing Solutions. Inc.
Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #:
21-19 County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparaon informatn:
Comments:
Dilution prep ('Y) 10.9S 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.
19S0 1402.5 700 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism Information:
Test information:
Organism age:
z -4At5.
CX..b Randomizing template:
o Date and times organisms
\\01-os er6%
', rcz I OCO Incubator number and were born between:
shelf location:
Organism source:
t-1S-0JS P YCT batch:
CI tOs Transfer bowl information:
pH =1.Al Temperature =
I Selenastrum batch:
1 6-oS Daily renewal information:
Day Date Test itlation, Control water Sample numbers Analyst renewal, or batch used used termination time mm__
0 iIoi I A to-%,L.056~ O51t%.O%- 402-A 3
.7-1 it-a2-om 515 0%sl o-zA1-c s
2 I~n-O 11IA
-lo- -O'SA
'1 C-%.
'7 Ak I
b-loiS
)1~!- ti6 Ni.~16z..
t\\
6 io-10 i l-oS-ose A sill~0a I Kb 7
Control Information:
1 2
Acceptance critedit Summary of test endpoints:
% of Male Adults:
S 2 00/
7-day LC50
> 1CM,
% Adults having 3 Broods:
/0CCIV ftV 2! SM/
NOEC I ON.
% Mortality:
OZ
! S 20%
LOEC j
.. t Mean Offspring/Female:
21
- 2.
k 15.0 Ospring/fenale ChV
> low,,
%CV:
4 -
<40.0 %
IC25>
1o
,4 Einn'ta;etn ouinIc I
Environmental Testing' Solutions, Inc.
Page 2 of 7 Species: Ceriodaphnia dubia Client: Seguovah Nuclear Plant - Non-treated Date:
l I-ok-OS CONTROL -1 Survival and Reproduction Data Replicate number Day 1
2 3
4 s
6 7
8
[9 10 I
Young produced 3
c0 0
a 0
C I 2
Young produced aC KI Adult mortality
?
l 2
Young produced 0
~
C)
. CX o
Adult mortality l
%,-L>
l-L lt Adult mortality f
3 Young produced IB I
O I O I
O C)
Adult mortality j
I t
I l
4 Young produced o
S Adult mortality I
_l_
l 1
=
=
S Young produced 13
-' 1 1
l
-tl mr ai
-=
_____l _l>
6
[Youngproduced
(
?
O
°l l
O0 l l I
Totalyonug produced l
2Zl2 P l IS l~
IS l4 31 l z2 21 lZ0l Final Adult Mortality* '
L L
r
' j L -
lr Xfor 3' Broods I X_
..c I>x I
- x.
I x
Note: Adult mortality (L live, D - dead) 4 S.T 69ec.
Concentration:
- % Mortality:
l 7
l Mean Offspring/Female:
21.1 1
CONC: 10.98%
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
3 9110 I
Young produced l 0 C
0 0
Q l
l° CD
°
)
I Adult mortality t
!L L-L.. !.
'i L.
L
. L.
I 2
Young produced l
l 0
Adultmoraty L-1_
3 Young produced [
O I O C) o C
0 I
o1 Adult mortality [
1~
L L
C7U 4 Young produced
~
3 7~
Adult mortality L
L-5 Yousg produced I Ij 1j 11°I I I i tI 01 '
.I.1 Adultmortality t_.
?
IL I.1 I
I 6
Young produced l IL f
l Q
l O l O Q l loIo Adult mortality L-I L
U U I L L
L L
7 Young produced 1l.
j:
Z I
5 l1o 133 L's IS IZ I Totalyoungproduced zI Z
o 21 l Z ZA9 l 3o 3o l 5
V.
Final Adult Mortality l
L..
l..
l' >
- l. 1r i
i Note: Adult mortality (L - live, D - dead)
Concentration:
Mortality:
0%.
I Mean Offspring/Female:
l2&.
% Reduction from Control:
- 2.S% I
I Environmental Testing Solutions, Inc.
Page 3 of 7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date:
I1-ol-OS cOaNC: 22%
Survival and Reproduction Data l
Replicate number Day l 1 2
3 4
S 6
7 S
9 10 I
Young produced C
J O
0 0
0 O
Adult mortality U.
L..
1 2
Young produced CS 0
Adult morta l
t l
L_-
L-tl>0>L00t 3
Young produced o 0 _
0 0
0 O
Adult mortality L-I-
L -
I § F
l...
- P ?
4 Young produced :
A LA S
jLA q
j Adult mortality L
L U.-
j LI.
S Youngproduced 0
.J.IL
... l Q.
I2.
l i
O Adult mortality L.
- l.
L.
l t
l-6 Young produced o
CX 1 zF I
0 I I
I l
I 0
I Z.O Adult mortality Lj k.-
L-
%-- I t
-L 7
Youngproduced l I a 15 I 3 I (.
1i 4 1'
I _3 Totalyoungproduced l Z9 I 31 3k ze l 3 3
l 33-T 2 I Z
28 Final Adult Mortality.
l T
7 1
1 1
l Note: Adult mortality (L live, D-dead)
AStIt S
t-l Concentration:
%Mortality:
l Mean Offspring/Female:
I
'fo c l
% Reduction from Control:
- E. 67 j CONC: 43.9%
Survival and Reproduction Data
[
Replicate number Day
_ I 2 1 3 4 1 5 6
(7
]
8 9
10 I
Youg prouced0 Q
0 ri
)
C 2
Young produced l7 Adult mortality
[
U UJ-t C
C
(_
2 Y o u n g p r o du c e d
.. C
..Cl O
- i.
- ~ L *O C )
I
)
l C 2
3 Young produced l
l Adult mortality I
Lk L*=
L-I'-
UL L
4 Young produced 1W l
1 j
lt l
Adult mortality l
L L -*
6 Young produced J
17 C
Adult mortality L-L-
L-L-
L-I.
-o gprdue I
-a
=
0
-=
C Adult mortality 7
I 6
Youngproduced. '
j
.0 i
Totalyoungproduced l331 30 31 2
l-32. 3Z l a
l 32 29l FinalAdult Mortality l
1t Note: Adult mortality (L - live, D - dead)
(AIM0 0PiA
.4-5?Lrr 6,=b Concentration:
% Mortality:
O, Mean Offspring/Female:
31.1
% Reduction from Control:
- j. 37
rvArnnmental Testing Solutions. Inc.
Page 4 of 7 JL'z,.. V D=---
=--
Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date:
%-oX-cy S CrfNC! 72%
Survival and Reproduction Data 1-1 Replicate number Day I
l 2
3 l4 5
6 T 7 a
9 10 Adult mortality 2
Young produced o
c o
i n
OT o
I O a
o Adult mortality L
1 1
t 3
Young produeed Ce-
- t.
Q d
Q..
Adult mortality I
t 4
Young produced Li C
1 S
0 Adult mortality j L-L LL L
-L S
Young produced a
1
=
0 l
c) l I
O Adult mortalIty 1_
?
U-L l
l-L 6
Young produced 13O V
I l OI I c:_
Z I Z I l(
0 I I Adult mortality l
I L-L-
L-I L
t-
=
=
7 Youngproduced l
Ii' It.I
,s 1-5 11 1 it.
I 1 Totalyoungproduced l 3s S
33 38F 0
31 l
T 5
Final Adult Mortality l
L_
l L
l LC-l 7 Note: Adult mortality (L - live, D - dead)
Concentration:
l %Moriality:3 0 O.^
I Mean Offspring/Female:
.32.(4 I
% Reduction from Control: l 11:17.
CONC: 100%
Survival and Reproduction Data I____
Replicate number Day 1
2 3
4 1 6 71 8
9 10 I
Youngproduced 0
C) l j0 l0 0
l ll Adult mortality l
U.
U.
l U J U -\\
L l
1 2
Y ung produced i
Adult mortality U.
W U
t 3
Young produced C) l C)
C)
L C5 QO Adult mortality j U
1 L..
1 t.-
4 Young produced lLi LS t
l S
l Adult mortality l
=u l
- J J-Iz L
l o n r d c d 0
4 2.
I )
L L
L L
A dult mortailty l L
l C 7 l7 T T l
t j l
Q l L
l 6
Young produced j
a
=
0 i
Adult mortality L-C L -
L 7
1 Young produced r, I I C) r 1
1 I
l I
Young produced aIa a
t-Totalyoung produced 7
I l
Zj 7 3"5 32 32.
34 7
1 Final Adult Mortality I l U
l C
l Note: Adult mortality (L - live, D - dead) -sfe tV0J_
Concentration:
%Mortality:
l Mean Offspring/Female:
l Sz.(,
% Reduction from Control:
- 1.17l
Environmental Testing Solutions, Inc.
Page 5 of 7 i ' "
Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant-Non-treated Date:
ul-Ol-OS CONTROL -2 Survival and Reproduction Data Repicate number Day 1
2 3
4 5
l6 7
8 9
10 I Young produced Q
O O
O C
C 0
Adult mortallty C
W 1
l 1
L I
t 2
Young produced C)
L Q
j.
O l
.C Adult mortality V-L-
L l
3 Young produced l
c) 0 O
n Q
Adult mortality 2
_4I _
V K L_
4 Young produced l
Adult mortality T \\
1 ll
=
==
_=
s Young produced I 0 l
I O
lI l
Adult mortality l
I-1 l-L l
6 Young produced C:)
o l
O l O I Z lO I
O Adult mortality L
l L
l L-
\\
7 Youngproduced i3 111 LY IILi 3
Jb 1
Total young produced l
%q li BI l'4 Z'1 l't 21 l2°1 3
FSnal Adult Mortality
, > l L
J t
L lI Note: Adult mortality (L - live, D - dcad)
Concentration:
l % Mortality:
l0%
Mean Offs ring/Female:
l CONC: 100% Intake Survival and Reproduction Data l l_Replicate number l Day l
1 2
l 3
l
- 4.
i 6
l 7
l 8
l 9
le0 I
Young produced C)
O f
(
l 0
a.1 C I..
c l
Day J____Adultmortality l
2 3
4 l
7 10 2
Young produced l
Qj l
l l
l)l l
lO l
l l
Adult mortality A
L-LI'!IT!iL L
3 Young produced Cl) l I
l lo
°
° i
l Adult mortality l
4 Young produced L A L
M i
i W
W I
TI l
Adult mortality _l (t 7 I I
l S
Youngproduced O
l l
l l
13
° lL l
Adult mortality L-L L
-L L
L I-'1 1
lITi loI1 b1<
Adult mortality L
L L
T L 7 5
S S7 U
5 7
Young produced 1(I IZ Total young produced V6 I3;- 1 31 1 ^35I 2'1 I 35 I I *q 3-1
__ty I C 1 IZ1 C-1 1
1
=1LK-J K1-Note: Adult mortality (L - live, D - dead)
Concentration:
%Mortality.
7, I Mean OffspringlFemale:
I 33.O
% Reduction from Control:
- IS.5%
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Veriflcation of Ceriodaphnia Reproduction Totals Control-I
-Day Repicate wnuiber
.11 2 l 3 1 4 5
7 8
9 is-TOWl 1
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 S
13 10 0
1011 1
O O
O O
45 6
0 0
9 0
0 9
10 11 11 11 61 7
14 13 13 1
16 11I 15 12 12 15 132 Total 32 28 26 25 31 24 30 26 26 29 27 10.98%
Day Re licate number 1
2 3
4 5
6 7
8 T
16 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 O
O O
O O
O 3
0 0
0 0
0 0
0 0
0 0
0 4
4 4
3 3
4 4
4 5
5 3
39 5
11 0 11 10 It 11 0
10 10 12 86 6
O II 0
0 0
0 11 0
0 0
22 7
12 15 13 16 13 15 15 12 15 11 137 Total 27 30 27 29 28 30 30 27 30 26 284 22%
Replicate nhrber
.ol Da 1 2 3
4 5
6 7 1 8 9
Ttl 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
O_
O O
O 0
O O
0 0
0 4
4 4
4 4
S 4
4 4
4 4
41 5
I11 0
0 2
0 0
12 0
1 1 36 6
0 12 14 10 10 10 0
12 0
S1 7
14 14 13 15 13 16 14 17 13 13 142 T3td 293 31 31 28 3
3329 28 310 43.9%
Day D
l11 2
3 4
5 16 17 8 i 9 lo0 TOWi 1
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0 1 0
0 0
4 4
5 4
3 5
4 4 1 4 5
4 42 5
11 10 2
00 0
0 0
12 O
35 6 L I-,
0 12 12 11 13 12 10 0
o 10 1
17 15 13 13 i6 1518 L16 1
15 153 Total 133 i301 31 2
32 32 34-30 132 129 311 72%
I~
-i 2
Re Ilcate nwn~r lTota l 1 2
l3 l4 l5 l
7 l8 l9 1 S I
I 0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3 _
0 0
0 0
0 0
0 0
0 0
0 4-4 5
6 4
4 4
4 5
4 4
44 5
0 12 0
0 0
0 0
0 13 0
25 4
13 0
10 11 13 12 12 14 0
11 96 7
19 I
14 18 14 14 15 17 16 19 161 Total 36 32 30 33 31 30 31 36 33 l34 l 3
100%
DRe Ilucater ber TOW I
l 2l 3
4 1 5 6
7 8
9 I0 Tlo I
0 0
0 0
0 0
0 0
0 o
0 0
2 0
0 0
0 0
0 0
0 01 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
5 4
4 4
6 4
4 4
5 44 5
0 1
12 10 0
0 0
0 13 131 49 6
14 11 0
0 14 11 12 12 0
0 74 7
1*7 15 17 131 Is 15 16 18 15 18 1 159 Total 35 32 33 T27 33 321 32 34 32 l36 326 Control-2 l a l
Re plicate nulber TOt 1
2 3
4 5
6 7
8 9 10 I1 0
0 0
0 0
0 0
0 0
° 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 3
5 4
4 5
4 3
4 4
4 40 5
I 10 12 9
11 11 0
10 0
0 11 74 6
0 01 0 1
0 12 0
9 1
0 32 7
13 17 14 16 13 13 16 14 14 15 145 TOtW 26l 34 27 31l 291 29 29 27 29 1 30 l 291 100% Intake Day
~
R laenunber Toda I Dy l 2
3 1 4R 5
6 7
8 9
10i I1 1
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0 1 0 0
0 0
0 0
0 0
0 0
4 5
5 4
4 4
5 4
4 4
4 43 5
0 0
11 0
0 13 0 10 1212 58 1 1 1 1 0
12 10 01 14 0
0 0
58 20 161619I I1 171 15 20 18 21 177 Total 36 321 311 351 29 351 33 34 34 37 336
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number 2178 Revelwed by: r 1
/_
Concentrathon Replicate anuber Survival Average reproduction Coeffideut of Percent redueo from
(%)
1 2
3 4
5 6
7 8
9 l0
(%)
(oefspring/temnle) aredo")
P"Wldcoal, (I
Control - 1 32 28 26 25 31 24 30 26 26 29 100 27.7 9.8 Not applicable 10.98%
27 30 27 29 28 30 30 27 30 26 100 28.4 5.6
-2.5 22%
29 30 31 31 28 30 31 33 29 28 100 30.0 5.2 83 43.9%
33 30 31 28 32 32 34 30 32 29 100 31.1 6.0
-123 72%
36 32 30 33 31 30 31 36 33 34 100 32.6 6.8
-17.7 100%
35 32 33 27 33 32 32 34 32 36 100 32.6 7.4
-17.7!
Control - 2 26 34 27 31 29 29 29 27 29 30 100 29.1 7.8 Not applIcable 100% Intakc 36 32 31 35 29 35 33 34 34 37 100 33.6 7.2
-5.51S Outfall 101:
Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
MSD =
PMSD -
2.148 7.8 Minimum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistical significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity test a toxicant reduces Ceriodaphnia reproduction by 9.7% from the controL Lower PMSD bound determined by USEPA (10' percentile) - 11%.
Upper PMSD bound determined by USEPA (90k percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
s when 1.821 63 USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Polutant Discharge Elimination Program. EPA-833-R400-003. US Environmental Protect Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Statistical Analyses C_ adapbisa Sunival md ~prsdawtl TtAMlzprsds Sart Dstc IUU20S Tat 11>.
CPRR SaMpl1D:
TVAI SQN Ou1 101, Ne-ted End Dade: II005 ab ID:
IU-Ravik Ttkg Sol Sa Impe :
DMR-Dbdearp RM-1egopot Sa lkDae:
hookw IWCHR-EPA421R.2.013 TeatSPc CDlendapbnkdubk Cse.%
1 2
3 4
S 6
7 a
9 la DC ol 32000 2OO 1OO 26.000 25.000 31.000 24.000 30.0 26.000 26.00 29.0DO 10.91 27.000 30.00D 27.000 29.000 2t.000 30.000 3M00D 27.oD 30.000 26.00 22 219.00 30.00 3
31.000 31.00 23.000 3Q000 31.000 33.000 29.000 21.0DO 43.9 33.0W 30.000 31.000 21.000 32.000 x
100 34.0X0 30.00 32OD0 29.ODO 72 36.000 32.000 30.0D0 33.000 31.000 30.000 31.000 36.000 33.000 34.000 100 31.000 31000 33.000 27.000 33.000 32.00D 32.0O0 34.000 32.000 36.00D Trasa=rm: Userafa ned I-Tanedb let Ce-%
Mas NMe Mea Min Max CV%
N 4-St CiksI MSD Ma.
N.Ma DCeol 27.70D ADMo 27.700 24.000 32.000 9.7u 10 30.400 Loxo 10.91 21.400 L.0253 21.400 26.000 30.000 3.355 10
.0.745 2.217 2.140 30.400 L.DX0 22 30.000 0.0300 30000 2S.000 33.000 5.212 10
- 2449 2.17 2U14 30.400 1.000 43.9 31.100 1.1227 31.100 2.0o0 34.000 5.951 10
- 3.620 2.27 214S 30.400 L.OX 72 32.600 1.1769 32.600 30.000 36.0D0 6.113 10
.3.217 2.217 2IU6 30.400 L1 100 32.600 1.1769 32600 27.000 3.000 7.402 1o
-5M7 2217 2.14U 30.400 1.0000 Anxilkm~Taeaft..
Sladatle Qitles skew I rr KowSlomwD Teat i (d > 0.0) 0.52866304 1.0L3 401339173 407714901 BDtleWs lktk deates eqaves
@(p-0.49) 4.433910234 15.00627224
=
elTe tet(1.4z LOS)
NOEC LOEC ChV TU Mon MSDp MSE MSB F.Prob n
t DMea nsDTeart 100
>100 1
2.147790172 0.077537551 43.24 4.411111111 1.00.6 5.34 Treamesb ne D.wfol Lsar Isteepaleas (3O Reumpla.)
Paint SD 95% C.
Skew IC0
>100 IC1O
>100 ICIS
>1oo 1.0 1C20
>100 0.0 lC25
>100 0.0 IC40
>100 0.7 IC50
>100 0.6 0.2
.3g02 0.1 0.0a 4.1
.0.2
........ e 0
20 40 s0 60 100 120 Do"e %
DaeRespepaa Plot sqn_11j1-0Sdata
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 01-08, 2005 Statistical Analyses Carbdaphals Survival and Repreductim Tut.Ropredavda Stut Dec W1M=
Toot D.
CdPRCR SipIe1I TVA/ SQN hlukcINmwcgtnd Ead Dae Ia2eo5 lAbMD MEav 3
nvk. TmtMgSol.
SMTp e:
DdRflchazp M.Rg Ppp Saml WCt Pmtoek P WCHP431AS21-RM-013 Tod Specie.:
CDCubdaphok ubk come.%
I 3
3 4
S 7
8 9
to D-Cacrol 26.000 34.000 27.000 31.000 29.000 29.000 29.000 27.000 29.000 303CC 100 36.0C0 32000 31.000 35.000 29.000 35.000 33.0 34.000 34.000 37.000 Trnduferm Uatraatmed I-Tae mefank C
M%
MeN 4 Mlnu M
Mi.
R.1 CM%
N wSt 0*dea MSD Me N-M.
D 2.100 1.0000 29.100 26.000 34.000 7.43 10 31;350 1.0000 100 33.600
.146 33000 29.000 37.000 7.111 10
.4.214 1.734 1.121 31350 1.0000 MAIxlmTeul Staed" e Ofta Sknw Kait 5upkO-Was wit leAialdcs aldietr nthou (9> 0.01) 0.96097127 0.161 0.0104966 0.153076601 F-Tat hadketes eqel awnce p - 0.)
1.1 17270827 6.541009335 Is Tst US)
USD*
MSDp MSB RISE F-preb dr RM~setTdutiet MadindicAteanouonidcaW dihuces 1.M454797 0.0629294s 101.25 551666667 4.5B.04 1.11 Twft0nbs v w D-ontrol L
bar Isterpentas (200 Rianuple)
Pelat SD 9%CL Skew
>100 1010
>100t lOIS
>100 1.
120
>100 0.
It25
>100 oA I040
>OO 0.7 IC50
>100 0.6 0.5 I 0.4 0.3 E 02 0.1 0.0
.0.1
.02 0
20 40 so e0 100 120 Caosan sC
_qGtpQSp*
4.,
35 30 J25 It 10 1,
4.10.05 lee of algrlfcnce U,I sp-I-O)-OSdata
Environmental Testine Solutions. Inc.
Page I of 6 Environmental Testing Solutions. Inc.
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: IVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #:
Z?-I County: Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information:
i Comments:
Dalutioa prep (%)
109S 22 1 43.9 72 100 Each concentration was treated Effluent volume (mL) 274.5 550 1097.5 1I00 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2225.5 1950 1402.5 700 0
to remove pathogenic Totl Volume (ML) 2500 2500 2500 2500 2500 Interferences.
Teas organism information:
Test information:
Organism age:
T4.S no 23 AWS Ob Randomizing template:
Btt Date and tinies organisms I0-% S I
-M SSWO Incubator number were born between:
Organism source:
ABS Poj9 Io-31s Artemia lot number..
fIC'tDM Transfer bowl information:
pH =
Temperature -
°C Total drying time:
24.-to#
1t Z.
Date/Timein: 11-at-oS0 t
.Z35 Average transfer volume:
I Date I Time out: 1-oq-o( 5 ZiL
.e:_
Oven temperature: _
lot' Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time O
ftlrCos V SOO 1 Z I 10 z4051!
5O0\\.0 uol C%0 0
l1-02-0s
-i 5
o rzZ3i lio-SB zlu
- Coz. 0 2
1l-c)-6is Aj5 Stk j
Z 3O i-oS SI oz2.l2At-13 3
al-tSie c&S MS65 1131 16.1-s 8a CGlo0-12 4 1' Jr3 4
Ilaja tS llSO Il68
\\sSAo lio o
s i.-s-r fi 8
________1 l1-cn-os A os1o4.eAtz 4L
\\
6 11-6 OLa5 IASL.
1 II
-M- 0l 5 o
OSlloLI V
.OL
_I 4" CC 0a::.
S tOSC A
1.:
-O
-LO
_ _A t.,.
.:"s 7_
__k~
1Ao~~
Control information:
I
--- -No Mortality:
. Average weight per initial larvae:
Average weight per surviving larvae:
Acceptance criteria Summary of test endpoints:
-S 20%S.
7-day LCso, IOC%
I -
NOEC 17
- 0.
0oSO 2 0.25 mg/larvae LOEC J > lOOZ ChV
! > IC0,70
_C2_ _
)e o07,
Environmental Testing Solutions, Inc.
Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Surviva Day CONTROL A
B C
D E
-10 10 lC
/a 0_
2 10
/0 10 l0o i.) 10 10
/0/0 4
-I
/10 lo 10 ID' 6
1 I 0
/0 10 t
C 10 1*0 10 t(
1I)
Pan weight (mg)
Color Identification.
Analyst: _11-h~o I(,Thz 14MA 160i ?5.M 8~q.OI 13-Pan + Larvae weight O
D 0
411
!,~
.Z'.l.
zItt It G
t Larvae weight (mg)
_A,
?-,
-0
.,6 I_ __
WeigtZ per Initial number of larvae (mg)
C Initial numbere oflarvae W
Averge Percent weight per reduction laitd:!
from control 69 number of (1%X) lilarvae 5g Page 2 of 6 Date:
ft-ol-as l and Growth Data 10.98%
22%
F G
H I
J K
L
/0
/0
/o 10
/0
/0 10
/0 IQ
/o
/0
/0
/0
/0
/C10 /0 0
1/010 io
/0
/0/0 0,0 10
/0 tO 10 tO I 0 oI 0
/0 t0 10
/0 to
/0
'0 TZ_
_i lo /o to 10 /C) IC (0
/1 10 15M q.3t iqSq /1q.71 lOq' LFISS0 2 az.&L 20.2.i 2l.t 21AS Zi.S3Z 7.14 SA1; S.4S.e
(-.qq 1..q0 (,.b
'A 0'
g0 All 01-I'%P Al JO
'3 1-I. 7.
- 0. -.3
-. -7.
ComMrent codes: c - clear, d = dead, fg - fLngus, k = killed, m = missing, sk = sick, sm - unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
A Calculations and data reviewed: 4..
Comments:
Environmental Testing Solutions, Inc..
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
11-Ol-OS
.__,.___Survival and Growth Data Day 43.9%
72%
100%
M N
0 P
Q R
S T
U V
W x
0
/0
_ /0 0o 10 IQ
/0 /
10 1
/O 10
/O /'0 /O lo 'O 10 10 2
Jo
/0
,o c) l O0
/
/
/1 I
/
O
/O
/O
/O 1
0O/
1O IQ 10 I O/
10
- 10.
/0
.10 0
1O 5
10
/
/0
/0 10 10
/0 lo
// '
'0 6
IC) /O/
/
C)
/O
/O 10 to
/0 t0 I0
/O 7__ _
/ 041 1/o
/ 0 / 0 /
to
/O /O
./
IC/C A - Pan weight(an Color Identitcabo:
4.U5£ 1 ° I1.4 I g5 i4 iq1.,4(p Iv1.Z' l
I 1 l5 15 1.30 gq*3 Analyst:
?UIl.4146
~
ql.-qj~
60Io3 45 8 - Pan + Larvae weight (mge)ys<)
22.t0 20-0 21-54k Zl'L-Q-% M1 Zo.£ 1
tg.to 1Z\\Z 2.0-L 21.40 22.10 2l.C3 Larvae weight (mg) - A - B
.S S5. q4 (Onl
(,.I% S.13 t.*Wo (.xal (#.5Z 6.q5 lo.27- (6.-40 b.SO Weight per Initial number V
Average Percent wreight per troeDctonto 0
50 t.o
.L IS
°LI
°I7° i
n t
i; r
o c
n t o nunmber of
(%)
larvae (m g)
Comnment codses: c = clear, d - dead, fg =fungus, k = killed, m
=missing, sk sick, srn unusually small, Ig t unusually large, d&r decanted and returned, w = wounded.
Calculations and data reviewed:< r..
Comments:
weg_
pe reu.o
_nta frmcnrlf-O o~-
0 40 l.%
0.A
_ubrf M Comn coe:_
-cer ed g=fnukklem-msig ik m-uuulysal
Environmental Testinz Solutions, Inc.
Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
Il-M-CS Survival and Growth Data Day 100% Intake l
Z AA BB 0
1_
_O
/O 0 0 I_
1
/ 0
/0 Q
I 30 IC)
/0
/_
3 I 0 IC
/O /O lo
_O
/0
/ 0 5
10 /010 1
6 IDOI C 0)0 A - Pan weight (mg)
Color identIfication: tL" tS 15 uq-Z3 q(,lO B - Pan + Larvae weight (mg)
ZL Zb ma 3
Analyst:
~a Larvae weight (mg) - A - B Weight per Initial number of larvae (mg)
-C I Iaitia number of larvae ;)
\\0
°
.)
0 Average j Percent weight per reduction Initial from control
)b1 number of M(%)
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r = decanted and returned, w - wounded.
Calculations and data reviewed: 4A
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated July 19-26, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespremdeas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project mawner: 2178 Rei'elwed by:
k~
Not for Co lpliance Assesame, Iotenal Labontory QC Csa tsewtl -s, _
ee 1..1 _t wr l
Fk A-P. eslgh B - *1 _ (Lwm La,.W M-(-/) Wdpsillurt Mmawseigin/
C_
Ofowrarwa W*Miuutmm M unwhl Mm. 0tha COVOeleSt of h
"ddl-kum k1m (we 06120(ne)
- A-1 xvwsfhm.@e 5-l not" of ause (*e
(%)
1 eMw _nhard "vses
_ui (w)
A 10 10 16.22 22.47 6.25 0.625 0.625 Contrl B
10 10 14.93 21.73 630 0.680 0.650 3.7 0680 100.0 0.650 17 Not applicable C
30 10 13.86 22.23 6.37 0.637 0.637 D
10 10 15.06 21.63 6.57 0.657 0.657 E
t0 10 14.99 21.9S 6.90 0.690 0.690 1P91 10 to 16.21 23.40 7.19 0.719 0.694 2.6 0.719 to0" 0.694 2.6..
G 10 10 14.77 21.53 6.76 0.676 0.676 H
10 so 15.94 22J6 6.92 0.692 0.692 I
10 to 14.37 20.29 5.92 0.592 0.S92-22%
X 10 10 14.59 21.01 6.9 0.649 0.637 5.0 0.649 100.0 0.637 LO 2.0 X
to I10 14.75 21.15 6.40 0.640 0.640 L
10 10 14.65 21312 6.67 0.667 0.667 M
10 10 16.09 22.67 6.S8 0.65S 0.655 439%
N I O 9
14.4 20.58 5.94 0.6 0.667 1.4 97.5 0.650 5.9 0.0 0
1 0 1 0 14.17 21.54 6.77 0.677 0.677 I__
I10 80 14.55 1
21.26 6.71 0.671 0.671 Q
10 10 14.54 20.27 3.73 0.573 0.573 72%
S 10 to 14,46 21.12 6.66 0
.666 0.640 7.1 0.666 00.0 0.640 7.1 8.5 S
I80 t0 14.27 20.96 6.69 0.669 0.669 T
10 t0 14.77 21.29 6.52 0.652 0.652 U
10 t0 14.57 21.02 6.45 0.645 0.645 100%
V I
10 15.07 21.69 6.62 0.662 0.649 1.5 0.662 100.0 6.649 1.5 0.1 W
I10 8 0 1630 22.70 6.40 0.640 0.640 X
10 10 14.53 21.03 6.50 0.650 0.650 y
10 10 15.88 22.52 6.64 0.664 0.664 100IOlslake Z
10 0
1530 22.16 6J 6 0.666 0.677 1.5 0.6L6 100.0 0.677 1.5 42 AA 10 80 84.23 20.97 6.74 0.674 0.674 BB I
10 10 16.19 23.04 6.95 0.685 0685 Owafll let.
Deerit's MSD valve:
PMSD:
Deanett's MSD value:
PMSD:
0.0517 0.0255 3.,
MSD-Minmum Significnt Dillerence PMSD Percent Minimm Significant Diffeace PMSD isa owters of est peecision. Tne PMSD is Me Minimum percent diffeence between the conled and
_reat that cs be declared statistically significant In a whole cffften toxicity test On aae*asIgificant differcee occur rEx nsengtulTesgSoio Inl. chnic toxicity ts when atoxica n ceslpAles gowth by t32% f teontrol (deteniedtrogh referem tolicant teatinau.
Lower PMSDbound detcined by USEPA(10t pacrdle) -9.4%.
Upper PMSD bound "eined by USEPA (90bh percentile) - 35%.
The lowr ad upper hounds wer calculated by the USEPA using 205 tes conducted from 19 laboratores for Pfsepales g in chronic referele toxican tests.
USEPA. 2000. Understanding and Accounltng for Method VadrabilmY in Whole Efilfat Toxicity Applications Under the National Pollutant Dischare Elinmnatiou Propan. EPA-833-R-00.003. US Envirmentaul Protection Agency, Cincdnwti. OIL
TVA I Sequoyah Nuclear Plant, Outfall 101 U V-treated November 01-08, 2005 Statistical Analyses Laral Flis Growth *ad Survival Tal.7 Day Growth StitDale:
II/U Test ID.
ppP tRR SampkID:
TVAI SQN Ou
- lOfiO1, W-tcd EndDate 11Nc2C05 lAblD:
KISE-i. T t gSoL Samplek)pe:
DMR.DkchaM pb f R epgpt Seapl Date Phatocot PW IR-SPA42M-R-0213 Ted Specis:
PP-Pirnplhla przouwl Cwc.%
1 1
3 4
D-Contol 0.6250
.6S00 0.6370 0.6570 10.9 06900 0.7190 0.6760 06920 22 05920 Q6490 06400 0.6670 43.9 065S0 0.5940 0.6770 0.6710 72 0.5730 0.6660 0.6690 Q6520 100 0.6450 06620 06400 to65w Trasftram Uarvausomed l-TaIl b.onlk C C.%
Meom NXMM Man Min Mar CV%
N
$5Slat 0 ldm MSD Mea 14-MM D-Centol 0.649t 1.0000 0.6498 06250 0.6E(0 3.709 4
0.6720 1.0000 10.9 0.6943 1.06t5 0.6943 Q6760 07190 2.511 4
-2.074 2410 0.0517 0.6720 L.XO 22 0.6370 0.9S04 0.6370 05920 0.6670 5.02S 4
0.594 2410 0.0517 0.6441 09534 43.9 A65C 1.0004 0.6500 0.5940 0.6770 5172 4
-0012 2.410 0.0517 0.6441 0.95t4 72 0.64O 0.9t50 Q6400 5730 0.6690 7.075 4
A454 2410 0.0517 0.6441 0.954 100 0.6493 0.9992 0.6493 0.6400 0,6620 1A52 4
0023 2410 0.0517 0.6441 Q95U AuzU7iuyTew SladIstic QId Skew lart Spo-WMilk Test htdicXa u adstbimp> 0.01)
QtS4263983 O.334
.1.0C94753 0.7604t6643 Batlo Tedt dicates equal vaancm p -0.25) 6.54905624 15.01627224 loythn Is Tatt(14ah LOS)
NOWC LOEC OhV TU ISD.
MSDp MSB MS63 P-Frob 4t Duntrn Tet 100
>100 1
0.051714098 0.079390762 0.001727475 0.0200903 0.14102772 5,1S Trabnaas ve D-Control Linear.terpatlea (200 waVmpla)
Point SD aSV
(
vp)
Skew 1005
>100 IC15
>100 C1$
>1OD 1.0 IC20
>100 0.9 102S
>100 IUAO
>100 07.
0.?
1050
>100 0.6iii 0.3 0.1
-0.2 O
20 40 go 8o 100 120 Do. %
D *-lsPas Flbt as 0.7 0.6 0.6 0 0.
,.. 0.3 0.1 of I
4safl 0.05 lsfcl 3fsin~wc 34.5 Il 0
A 2a V
vi 8
sqdl-O1.05data
TVA I Sequoyah Nuclear Plant, Intake W -treated November 01-08,2005 Statistical Analyses L11vl Fisk G4wh uAnviv Twt-7 Day Gqwh StedDate.
IL2X5 TtstlD:
PpFRCR S&=pit TVAISQN ble, UW eated End Dae ILJ.V2005 Lab 1D KImvk.TeathgSol Sampe 7ypc D
bUR g
ko gRa t
Sampk DML Potb FWCEL41PA421-R4t2413 Test Spe=s:
P1-tpbha Pn as CaaC.%
1 2
3 4
D-Control 0.6250 61C0 0.6370 0.6570 1OD 0.6640 0,6160 0.6740 0.6150 Trasrorm Uafrwstorm 1-Taled hU~ek C.cn-%
Mea K-MeS Me" Miu Mu CV%
N t
Critica MD Mesa NMsa D-Conrol 0.6491 I.06D 0.6491 0.6250 0.680D 3.709 4
0.6635 I1.0D 100 0.6773 1.0423 0.6773 0.6640 0.6160 1.532 4
0.096 1.943 0.0255 0.6635 I.OOD AmWilisy Twt StMtRt CrtIc Skew Ketl Shapko-Wlls Tast kidWtesnorsadms bztuti p
(> 0.01) 0.954609606 0749 03675074 0.143961767 F-Tet imdta quv me5
- 02 5.399690151 47.46722794 Hypohsisst(14Lt9a5)
MSDx MSDp MSB Mt F-Pus dt lomecedaftt st Tatdke slgniflno tdikerudca 0602549333 0.039236367 0.0015125 0.00425 0.080913521 1.6 Tmaknent; n DContwol Iiseasr batnxatiaa (CM Reasuple)
Peint SD
- 5%
AQ5zp)
Skew IC10
>100 ICIO
>00D 0213
>100 1.0 IC20
>100 1C25
>1D00.
1C40
>100 0.8 IC50
>00*
0.7 0.6I0.6 0.4 a
0.3 0.2 0.1 0.0
- 0.1 0
20 40 so e0 100 120 Des-Iepoe PhI
(
0.7.
069 0183 0.67II0.96 10.65 0.64 P. 0.63 0692 0.61 0.6I 1-Intl 0.06 low oalgrnlc 0.58 4.
8 sqn-11.O-5Odawa
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephakespromelas Daily Chemical Analyses Project number.
2178 Concentration Parameter
zz1zz Initla Parneei
- y PH(SU)
.9-DO (mr/L)
Da4 Di final Initial nal Initial 7.381 7.87 31 7.70 7.0 7.7 7.3 7.5 314_l11 313
_58 58 95 93 B l-Control Conductivity (umhos/em) 4-Alkalinity (mg/L CaCO%)
Hdness (mgIl CaCO,)
He
^.
,1
^,
,1 Temperature (C) 24.6 24.01 24.81 24.3 24.Yl 24.)
4.1 24.01 2485 4.2 240l 24 4.!
2 4.2 PR (SU) 7.87 7.60 7.901 7.34 7.80 7.34 7.87 7.34 7.64 7.28 7561 7.98 8.14 7.80 10.98%
DO (-gL) 8.0 7.8 8.11 7.0 7.9 7.2 7.8 6.9 7.6 7.1 7.4 7.0 7.8 73 Conductivity (pnhos/cmn) 295 298 288 29829 295 296 Temperature (C) 24.6 24.6 24.9 247 25.0 24.4 24.7 24.6 24.9 24.4 24.8 2427 pR (SU) 7.86 7.61 7.84 7.43 737 7.34 7.84 7.33 7.64 7.29 7.56 802 8.14 77 22%
DO (mgiL) 8.1 7.8 8.1 7.1 78 7.1 7.9 6.8 7.7 7.3 7.4 71
.8 72 Conductivity (iumhostem) 284 292
_______ 27_
_284_
280 283 281 Temperature (IC) 24.6 24.6 24.9 245 25.0 24.41 247 244 24.9 24.4 24.8 24 248 24.2 Hll MS 7.83 7.59 7.81 7.44 7.71 7.33 7.80 730 7.62 7.30 7.54 80 8.14 7.86 HDO (mg/L) 8.3 7.8 8.0 7.2 7.8 7.0 8.0 68E 7.7 7.5 7.5 7
7.8 7.5 Conductivity (tjmhos/cm) 256 264
_25_
256 25 3 247 Temperature C) 24.7 24.7 24.9 24.6 25.0 24.6 24.8 24.7 25.1 24.1 24.7 24 24.6 24.3 pH(SOJ 7.81 7.63 7.79 7.46 7.69 7.32 7.76 7.30 7.61 7.28 7.50 80 8.14 7.86 72%
DO (ingL) 8.3 7.9 8.0 7.3 7.8
- 6.
8.0 7.0 7.6 7.2 7.6 7.2 7.9 7.4 Conductivity Otmhoulem) 219 2217 218 218 212 Temnperature CC) 24.8 24.6 25.0
- 24.
25.1
- 24.
24.9 24.7 25.1 24.3 24.7 4.3 24.6 24.3 p (SU) 7.79 7.61 7.77 7.47 7.66 7.33 7.73 7.29 7.59 7.31 7.47 8.06 8.16 7.89 DO (me/L) 8.3 8.0 7.9 7.0 7.6 6.8 7.8 7.0 7.6 7.4 7.6 7.3 7.9 7.5 Conductivity (Emhos/cm) 1841 17 7
191 100%
Alkalinity (Mng/L CaCO3) 631 67 Hardness (mg/L CaCO3) 751 63 Total Residual Chlorine (mztL)
<o.i0_
<0.10 Temperature (C) 25.0 24.7 25.0 24.6 25.1 24.3 24.9 24.6 25.2 24.3 24.7 24.6 24.7 24.2 H (SU) 7.76 7.61 7.771 7.451 7.64 7.67 7.31 7.57 7.34 7.44 8.10 8.16 7.86 DO (m&UL) 8.3 7.8 8.1 7.3 7.5 7.0 7.6 7.0 7.6 7.2 7.6 7.4 7.9 7.3 Conductivity (tumhos/cm) 182 i18 179
_88 100% Intake Alkalinity (mg/L CaCO3) 66 661 6
Hardness (mgrL CaCO,)
73 73 75 Total Residual Chlorie (m_
<0.10 1_
<.10 Temperature ('C) 25.1 24.7
.25.1 24.6 25.01 24.5 24.8 24.7
- 25.
24.1 24.8 24.4 24.8 24.4
Environmental Testing Solutions, Inc.
Page 5 of 6 Environmental Testing Solutions, Inc.
Page 5 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated DaJH Chemistry:
Date:
1i-o1 OS I
Day I
1-Analyst Concentration I Parameter CONTROL HAS0 pH (S.U.)
r--
ii DO (mg/L)
Conductivity (imhoslcm)
Alkalinity (mg CaCO 1/L) 9 Hardness (mg CaCO4/L)
Temperature (C) pH (S.U.)
- ¢.5> L 10.98%
DO (mg/L)
&.O Conductivity 2 96 i (mhos/cm)
Temperature ()
A4.b (a pH (S.U.)
22%
DO (mff/Ll IF./
B-i Conductivity 2S Y
TemDerature (°C)
- 24. (eA 74.q t;.0 I-'. -
pH (S.U.)
18
+
I5S 7-W Yl*:J f#2 3 3 43%
DO L)
.e s
.2 9
Conductivity 2~
Temperature ('C)
I Lz
.9 2q.
2 lZt.
pH (S.U.)
-it/3 4
,-9-;.
914z
[,
aZ7 72%
DO (mL) 3,3_G Conductivity 2/9
_2__
Temperature (C) 7gg
-l Z
° _
- 2. 3 pH (S.U.)
.* 7I 100%
DO (mglL) 8.5 Conductivity
/ ;?
(imhos/cm)
Alkalinity (mg CaCO3lL) b05 Hardness (mg CaCO3/L)
)
WWI PEWI I
Maui Temperature (C) 2S.0 PR(S.U.)=
-e r
100% Intake DO (mgtL)
Conductivity (Ilmhostcm)
° Alkalinity (mg CaC03/L)
J Hardness (mg CaCO3/L) 1 TR chlorine
<0.1 C Temperature ('C) 2'74.1 I
ffawI
_WI_
Mag I
Zq. s I
Initial F
inl Finali
Environmental Testing Solutions, Inc.
Page 6 of 6 Environmental Testing Solutions, Inc.
Page 6 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date:
Concen-Parameter tration I
CONTROL OH(S.U)
DO (me/L)
Conductivity (limhos/cm)
Alkalinity I
(mg CaCO3/L) 5__9 Hardness I
(mg CaCO3/L) 1 Temperature (0C)
~ ~
pH (S.U.)
10.98%
DO (mr/L)
\\ =_
s
^
v Conductivity (unmhosicm)
\\
Temperature (C) pH (S.U.)
22%
DO (mn/L)
Conductivity (umhos/cm)
Temperature (°C nH (S.U.)
43.9%
DO (mg/LI
,_-_I
_- l a
I ConductIvity 3
(j+/-mhos/cm) 12____
Z-!
Temperature (C)
TO.1 a4.i
,24.q
- 20. (0
!
_
I, __ ' -
nH (SU S~ co5 w.
l
q
-4 0
1 -1 :V_1 2
lf2-0 =A-r w 1 1-1 i
72%
DO (ma/L)
Conductivity 2t 7
- -6 ON
(;Lmhos/cm)
Temperature (°C)
-L.lQ 7Q.1
'0-
pH (S.U.)
t 100%
DO (mg/L)
Conductivity (umhos/cm) 100%
Intake
- t.
,.s
.'S.-
Alkalinity (mg CaCO 3/L)
Hardness (mg CaCO3/L) i : Iq l 40.10 S-L TR Chlorine (mglL)
Temperature (C) s pH (S.U.)
pa (s.u.)
x r
l R
=
w l -
DO (mg/L)
Conductivity (Umhosfcm)
Alkalinity (mg CaCO 1/L)
Hardness (mg CaCOA)
TMperature MI w
100 (0.I zs.I I
Initial
, Bp ture (°C) 74.q I z(l.oy I
Z.'(
_I L Final P Initial 14 Final I
-A C-xi os Det"
-tTwz 0,4
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 01-08, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubra Daily Chemical Analyses Project number 2178 Reviewed iy Dai D
6I r
n..
I*
I l*...I X
Concentration Irarameter
i1nitIa 4
- 4.......
nH (SU)
._. 4 DO (nimIL) l Conductivity (umhos/cml Control W§=S
..........4 Alkalinity (mg/L C*CO3)
Hardnes (nieL CaCO.)
4-Temperature (OC) 24.71 24.91 24.61 25.1 VI VI pH (SU) 7.87 7.74 7.90 7.62 7.80 7.65 7.87 7.54 7.64 7.49 7.56 8.09 8.14 7.97 10.98%
DO (mWpL) 8.0 7.9 8.1 7.5 7.9 7.5 7.8 7.4 7.6 7.6 7A 77 7.8 7
Conductivity (pmh/cm) 295 298
_288
_ _29_
8 292 295 296 Temperature(C) 24.8 24.7 24.4 25.0 24.7 24.9 24.9 25.2 25.1 24.6 24.7 253 25.1 24 H (SU) 7.86 7.72 7.84 7.61 7.73 7.63 7.84 7.54 7.64 7.50 7.56 810 8.14 797 22%
DO (m L) 8.1 8.0 8.1 7.7 7.8 7.5 7.9 7A 7.7 7.6 7.4 7
7.8 7.7 Conductivity (pnmhos/cm) 284 292 279
_8 280 283 281
_TemperatureiC) 24.8 24.7 24.5 25.3 24.8 25.0 24.9 25.2 25.11 24.9 247 252 25.1 24.9 nH (SU) 7.8l 7.7ALI-7.tI 7.62i 7.7 7.6 I I 7.8 7531 7.621 7.51 1 7.S 8.1 R.I 43.9%
DO (mnwL)
I 8.3 8.01 8.0 7.8 7.8 7.5 8.0 7.5 7.7II 7.1 7.5
- 7.
7.8 7.'
Conductivity (inmhos/m) 256 1 264 l 254 E_
256 253 253 247p Temnerature (Cl pH (SU) 72%
DO (miwL) oCnductivity (jtmhosjcm=
Teprtr E
tll (SU) 4-JO (mnLwL)
Conductivlty (umhos/cm) 100%
UkAalinity (mgfL CaCO3)
Iardness (mnlL CaCO,)
rotal Residual Chlorine (muiDLL rTemperature (eC 24.81 24.81 24.61 25.01 24.91 24.9_
7.791 7.731 7.771 76 7.6-61 7.561
-8.3 7.9 7.91
- 7.
7.6 7.5_
184 llll2 184 S3lE 182 lEhE 63 lllllll~l l~ll 62 1lll
-75 85 24.8 24.91 24.6 24' 25.1 24.8 7.761 7.73 7.771 75 7.641.
7.541_
8.31 7.81_
8.1
- 7.
517.
7.5_
182 18 79 66 lllllllll0lllll 61 llll 73
__73 24.81 24.81_
24 24.5 24.81 24.9 25.21 25 7.47 7.
7A4
<0.
25.3 2!
7A8 7.
7.4 7
<0.
25.1 24 I-
,lil (SU) t-
)O f(ui/L)
Conductivity (umhos/cmi 100% Intake Ukalinity (nr/L C-CO,)
lardness (nw/L CaCO.)
rotal Residual Chlorine (melLI
,,_,,,__,___, _......._ \\..'F{SL a
3Environmental Testing Solutions, Inc.
Page 6 of 7
- H&.
i-..
r.-,
1 Species: Ceriodaphnia dubla I
-.~
Client: SeauoYah Nuclear Plant - Non-treated
. Daiy Chemistry:
Concentration Parameter CONTROL pH (S.U.)
DO (mg/L) 5 A
Conductivity
~(1lmhos/cm).
Alkalinity (mg CaCO3]L)
Hardness (mg CaCO3/L)
Temperature (QC) 2 pH (S.U.)
10.98%
DO (mg/L)
O I Conductivity
-I,
r Date:
1%*
O\\k-Day I
. \\
9.'.CS7 A
I I
17 1 2SS 1
129
.9 (jimhos/cm)
Temperature (°C) 2Q4.
7-,*. A
-74.1 7z4.9 pH (S.U.)
It. V(a 4z
}
22%
DO (mg/L) 1 5.O 4L.
I Conductivit (gzmhos/cm) 2YY 2
Temperature (C Zl-.k 14.1 e. S ZS
- j. e 2s CS pH (S.U.)
T3 i.-
Y
. 9: 'A
- 6 43.9%
DO (mg/L) 8-3 Conductivity 2
(limhos/cm)
Temperature (tC)
F4
.1 f
q 5
i pH (S.U.)
3
?-.o f1 I-fiZZ
_ _ 6 72%
DO (mglL) 0..
. AC.
Conductivity (gimhoslcm)
Temperature (°C)
A.
7.*.
O I
pH (S.U.)
q-&a
- w. -G e
II I
Ii -
I. -4-q-'-
A--
- 0-100%
DO (mgWL) a I
- 1-Conductivity (amhos/cm)
I 11I/9Z Alkalinity (mg CaCOA)L)
Hardness (mg CaCO3/L)
<0.16
(
gL)
I A
es
-. o.10 Temperature (°C)
I IN.f5 I
I zq I
i-i pH (S.U.)
I 100% Intake DO (mg/L) 3 Conductivity
/ S7 z(gimhos/cm)
Alkalinity (mg CaCO3/1)
Hardness (mg CaCO3IL TR chlorine 4 0, 0
(mg/L)
.A
_ -Y
.~-6 I
- i.6 I.5 A! _
/ Z
_( _
-1 Temperature (°C) 7q. p Z7. L I
h Initial I
Final I
- a_
w1uta Z I
I:I......
Ii':
Il,.
Conc:en-.....
t.a:-,
CONTROL 11:P Environmental Testig S n
Page 7 of 7 Species: Ceriodaphnia dubia Client: Secuovah Nuclear Plant - Non-treated Date:
il-O 1-OS I..I.
Day I
Analyst pH (S.U.)
DO (mg/L)
_~~I
_-x--'---
Conductivity (gimhos/cm)
Alkalinity (mg CaCOWLI Hardness 7
(mg CaCO3/L)
Temperature (MC:)
-t I-.
I ii.
ii pH (S.U.)
I.
10.98%
DO (mglL)
I
.LU I
Conductivity (Amhos/cm)
I j
Temnerature (C)
-~L4q.
pH (S.U.)
1+-
22/
-DO (mgJL)
Ct-9 Conductivity I
(lgmhostcm)
Q) I Temperature (°C) pH (S.U.)
43.9%
DO (mg/U Conductivity I
(umhos/cm)
LaCf12 Temperature (MCI 71.-I I -S.
z-pR (S.U.)
I 4.5D 72%
DO (mgtL) 4D60 Conductivity (I~mhos/cm)
Temperature (C) pH (S.U.)
100%
DO (mgtL) 9 Conductivity u
(pmhoslcm)
I' Alkalinity (mg CaCO
^
Hardness (mg CaCOA)
Temperature (°C) pH (S.U.)
\\
100%
Intake DO (mg/L)
-1;I
'C.)
Zu
~.(p IL,
_5 Conductivity (imhostcm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO/L) 3w<0.10 Z
S.w 1
.6 1 100 MM 15 KR<. 1 I
ZY.1 7.
ai I
Initial II Fins 7-4 Zl
- h 5
4 I 'L S. 2-
- -Z4J
-7.t4.
5i:./l/
1s/(
I.,lo 17 i Al 117 I
Tempernture (0C)
M_
MS.7. I t 0
rl Final Inital Fin
_ A 45 VA, v
CL"&.&-
~~.
TVA I Sequoyah Nuclear Plant, Outfall. 101 U V-treated November 01-08, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Project numbter. 2178 Reviewed by: 4 u
.0 Concentration Parameter DavO Da I Dc 2 Day 3 Da 4 Day 5 Da 6'
__________Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.71 7.64 7.74 7.47 7.61 7.38 7.61 7.31 7.59 7.31 7.57 8.03 8.11
'7.79 Cnrl DO (m!L) 8.3 8.0 8.1 7.6 7.6 6.9 7.2 6.8 7.6 7.3 7.7 7.6 7.8 1
7.3 Conductivity ftamhoslcnt) 3(
1 998 298302 29
________Temperature CC) 24.7 24.5.
24.9 24.8 25.0 24.5 24.9 24.6 24.8 24.1 24.8 24.5 25.0 24.3 pH(SU) 7.77 7.67 7.751 7501 7.61 7.35 7.59 730 7.50 7.31 7.57 8.04 8.13 78 10.98%
DO (MaJL) 8.3 7.9 8.3 751 7.5 6.9 7.3 69 7.7 7.21
- 7.
7.5 7.77.
Conductivity (phsc)297 30291___
295__
294 297 28.7
_______Tenmitrature (C) 24.9 24.5 25.0 24 25.1 24.4 25.1 246 24.8 24 24.9 24.4 29 2.
pH(SU) 7.75 7.63 7.77 75!
61 7.37 7.57 73 7.50 7.1 7.57 7.9 812 ~7 22%
DO (mg/L) 8.3 7.7, 8.3 76 7
6.9 7.4 70 77 7
7.6 7.577 Cnductivity (pimhou/cm) 28.2 292 279___
291__
282 286 275
_______Tmperature CC) 24.9 246 21 24.5 25.1 27 25.1 24.5 24.8 26 24.91 24.6 24.9 24 pH (SU) 7.75 762 78 7.52 7.60 736 55 7.31 7.49 7.30 7.55 8.04 8.13 70 DO (fwjL) 8.3 78 83 7.6 79 9
7.4 7.0 7.7 7.1 7.7 7.3 77 7
49%
Conductivity (Pinhog/cm) 259 261252
___2_
254257f
~
252
_______Temperature (C) 24.9 24.6 25.1 24.6 25.2 27 25.2 24.5 24.9 24.3 24.9 24.3 24.8 24.1 pH (SU) 7.73 7.68 7.78 7.52 7.59 7411 7.53 7.29 7.49 78 7.53 8.06 8.14 7.85 72%
DO (mF/L) 8.2 7.7 8.1 7.8 80 0
7.4 7.0 7.7 747.5 7.3 7.8 7.3 Conductivity (Iinmhog/cm) 218 226 216__
222__
221 22220
_______Temperaiture CC) 25.1 24 25.2 24 25.2 24 25.2 24 25.1 24 25.0 24.5 28 -
24.2 pH (SU) 7.72 765 78 752 59 738 50 72748 73 7.5!1
.0 814 7.85 10%
O (migL) 8.3 7
8.1 77 9
71 5
70 9
7 7.6 7.3 8 7.2 Conductivity (pmluos/cm) 186 193 13 1185 182
________Temperature ec) 25.31 2461 2531 2461 25.2 246 252 26 25.1 23 24.9 245 24.9 24.1 il (SU) 7.711 7.641 7.791 7521 7.56 7.3 7431 727 7.48 73 7.501 8.061 8.18 7.83 100% Intake DO (mgtIL) 8.31 7.7 8.I 74 7.8 7.0 6 718 727.61 7.41 8.0 7.0
-Conductivity (amnhesikm) 191pn I
______17 173__
11(2 180
~13 Te eaue25.21 24.6 25.124 25.1 24.651 25 20242 25.11 24.51 24.91 24.11
FVnvironnmental Testiniz Solutions. Inc.
Page 5 of 6 t
Ss v
c
_ge Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
il 06 Dafly Chemistry:
I Day I
Analyst Concentration I Parteter I__
F CONTROL UH (S.U.)
DO (mg/L)
Conductivity (Amhos/cm)
Temperature (C) pH (S.U.)
10.98%
DO (mgL) 3 Conductivity 2
(PImhbs/cm)
Temperature (C) 2L1.
pH (S.U.)
S t2%
DO (mg/L) 2B 3.
Conductivity 2 g (mhos/cm)
Temperature (°C)
S pH (SU.)
}
7 43.9%
DO (mg/L) 83 Conduetivity oZ (Aimhos/cm) /
-9 Temperature (eC)
>,cL pH (S.U.)
I. 723 72%
DO (mgfL)
&. 2 Conductivity
,ll Temperature (°C)
_51 pH (S.U.)
a_ ~
100%
DO (mg/L)
~*
s Conductivity (gmhos/em)
Temperature (C)
171
7U
pH (S.U.)
7 9'/
1 -7 Co IV 9- -
I 9 2
I *.S 100% Intake DO (mg/L)
Conductivity (Iimhos/cm)
&3
'. 't I
0I. 1 17/
{~ J O 191.q 1 :,4
/?I i
M ^
s Temnerature 1C) 71.2L 2S,l 2Z. 6 A
I g
-Initial I
I - Initial I
Final I
Initial Flinl
Page 6 of 6 Environmental Testing Solutions, Inc.
Environmental Testin2 Solutions, Inc.
Page 6 of 6 Species: Pimephatespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
ii- 01-OS UI I
Anals I
Concen-Parameter tration I
CONTROL PH (S.U.)
DO (mg/L)
Conductivity (Amhos/cm)
Temperature (°C)
S P
f 10.98%
DO (mg/L)
Conductivity I
(gmhostcm) _
Temperature (°C) pH (S. U )_
t 22%
DO (mg/L)
Conductivity
(,umhos/cm)
'Temperature (°C) 2 pH (S.U) 5s-43.9%
DO (mg/L)
/
Conductivity (tumhos/cm)
Temperature (C) zS.-2 pH (S.U.)
- S2 72%
DO (mg/L)
Conductivity
(,Lmhos/cm) 2-22 Temperature (°C) 2S.Z-
-pH (S.U.)
.S 100%
-DO Conductivity i01mhos/cm) s I
I Temperature (IC)
Z
'4. I s 7.5 Z.5q 24zS
- 24. B A9 7-t T--- 1s '7 s tS.
24.2 L-.
- 7. J3q
-7.6
- 7.SO M' 7.!i 2 S.O0 1
7.51 t 7. 07 1:
u
_s
,,_i 2z(.1 I
11
- l r-Z..
v_
?
- 5 LZ 74.2-
- -95 T. _Z
-Z14.1 F. 93
- .o pH (S.U.)
4 100%
Intake DO (mglL)
I 7.a I7-.
Conductivity
?5 (Mmhos/cm) __
l8z a00 Temntrgtuer& t'C' X.
1 7L1 C 2c.
I no I _
- I%* I J
c-
_ Ia L
Ia
. a.
I N
.-t. a I
L
.- I I
XI. I Initial Final Initial I
Final I
Initial
-4Final
_-)4nitlal 4: Final m
-4C A+,
CAg PA
Environmental Testing Solutions, Inc.
t "
Page L/ of.
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Jq99,:&
Date analyzed I //- o/-o5 Iodide reagent: I 1
?
6-K2 Acid reagent:
/VA/6 Z
Calibration:
l A
1§;i 0.10 mIzL I
1.00 mg/L I
Reference standard number
.T1 7S53Z5 S
- Jo4 s3 I
Note: For samples with a residual chlorine of > 1.0 mg/L the calibiation range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS - MV/TV x 100
.number (mgtL)
(mg/L)
(acceptable range - 90 to I110%)
3.r S5 2 0.50 O -- /5 3/0 2. C /
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD - ((S -D) /[(S+D)/211 x 100 number (mg/L)
(acceptable range -
- 10%)
- obSDo1.o2 J-Z4TF-zocr
'o^.
s S eo. o2/2.
Duplicate DZe. o57 _ _
Sam Ie measurements:
Sample Sample ID Sample characteristics Residual chlorine number
- m/Ll Blank (shouldbe-<O.IOmew W
%a.O 2?2 J.5LQ3L Fi#) -/0/-
iv ez-e (c'l tat C
o$
Zf Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratosv control standard:
Reference standard True value (TV)
Measured value (MV)
% RS -MV /TV x 100 number (g(mg (mngWL)
(acceptable range - 90 to 110%)
- 7-1S55 0.50 O S/O
/02-a j/
Reniewed by Date reviewed I lNWr-I I
I %,) - O' I
'Y'7-S %
Environmtental Testing Solutions. Inc.
-- I--
.1~ --.,,", -, -~-- - - - - --.
P ag e -6 7 --
Page..I.. of
/
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mgtL Meter Accutmet Model AR25 pEVlon Meter Iodide reagent: l ///
Z 0 JZ
)
Acid reagent: I /7IZ c1 g
K III Analyst l Lj9 Date analyzed I //-7 3 -o I
Calibration:
J'Qw*'kr7U-M.
.gl 0.10 mg/L I
1.00 mOg I Reference standard number l I S3Z S--
IZ S 3 4 Note: For samples with a residual chlorine of > O.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
- Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS - MV / TV x 100 number (mgtL)
(mgIL)
(acceptable range - 90 to 110%)t fI5 02 I.50 Duslicate sample precision:
Sample Sampli ID Sample characteristics Residual chlorine
%RPD - {(S - D) /[(S+D)/21) X 100 number (mg/L)
(acceptable range - a 10%)
4, uplicate I
Samvie measurements.
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
-Blank (should be =O.
I0mglL) i u6
-N
- O
. 08 i/
CosiO3.\\
oX CS A1 l~te go9-J4iyA 7e//i cG~.
to.oos°zC, Note: All samples were analyzed in excess of EpA recomnmended holing time (IS miues unles otheris nted 11sl nute
.s cw se no edX.
w &
Aw
.o Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS MV TV x 100 number (mdgL)
(mg/L)
(acceptable range - 90 to 110%/*)
L
,i6532*
0.50
°. 5, 1/
Reviewed by[
a l
Date reviewed I It <3O 1
Environmental Testing Solutions, Inc.
Page I of I Total Residual Chlorine (EPA Method 3305)
Matrix: Water MDL = 0.10 mg/L Meter Accumet Model AR25 pH/Ion Meter Analyst Date analyzed [
I Iodide reagent: [
I MAjjf j Acid reagent: I IA ki toe I
I Calibration:
I 0.10 mgiL I
1.00 mg/L I
Reference standard number I
s5sa2S I
.lij.s_
s I Note: For samples with a residual chlorine of > 1.0 mg/L. the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS - MV / TV x 100 number.
(megL)
(mg/L)
(acceptable range - 90 to I 10%)
IA Ss 5aS 0.50
- 0. tO)3 I
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD - {(S - D) /[(S+D)/2]) I 100 number TV A
- mgL)
(acceptable range -
- 10%)
105b4.
) S IQi S
o0l4 I1 1oDuplicate D 4.o-0 '4l 2.
Samok measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mg/L) 44n)
Blank (shouldbec<0.I0mg/L)
'0o.ooqqJ rkl5Ii o1z V A-a S¢*e-w sO. O atdS CS tI C6 LI P
Zo. C o aZl-OSl s.D no COL2. CUO. c 30 O6ilDOS.tI SCmLdl Ett kiF l
r f M
CLN 4 d 14 bL
_ ah Lw ILD0 oS009?
.. o.ZZI Note: All samples wereanalyzed in excess of EPA recommnended holding time (15 minutes) unless otherwise noted.
Laboratory control standard.
Reference standard True value (TV)
Measured value (MV)
% RS - MV / TV x 100 number (ngfL)
(mg/L)
(acceptable range - 90 to 110%)
j Ss 5
0
.50 0.02.
9 Reviewed by r
=.A 5-J Date reviewed l (i&D4S
Page
(,oO..
Page I of I
Environmental Testing Solutions, Inc.
Alkalinity (EPAMethod310.1)
',61stod: 16 O Matrix: Water, MDL = 1.0 mg CaCO3JL
,g Analyst r
ed.12 Date analyzed Titrate samples to pH = 4.50 S.U.
Titrant normality and multiplier termination:
pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na:CO3 x 0.05)/E (V s 50000)/ 100 ml sample water reference standard ml ml ml
- 0.25/E
-.Vx 500 4.5 S.U.
number number (E)
(acceptable range - 0.018 - 0.022)
%J4..
ipJ IJS LA oR
__0 12_ lt2 a
Q to.2 (31jVAa%00- F0J 0.Om 0.012015 Laboratory control standard:
Reference True value Sample Alkalinity (1V)
% RS -=IV
/ TV s lO0 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L)
(acceptable range (mg CaCO,/L)
(ml ml ml ml
-90 to 110%)
IN AI lo0.
10 1-2.z 21.6 I.t 10-CGi56'7 Duplicate sam e precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L)
((S -D) /[(S+D)y21l x 100 number (ml) ml ml ml (acceptble range 1o%)
io 6aStAo4,b iOo 42l. 8 Qq 3.1 lo.2 s
32 Duplicate
-2e.0 O l
D
, 3
.~
~
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOjL)
[(Mg CCO 3 L) ZIl)Zml mll ml
~j D
lo 2
-13249 *'
1 A2.
SZ i
Sample alkalinity (B)
Measured spike value (MV)
% R - MV /SVx 100 (mg CaCO,/L)
MV - A - B (acceptable range (mg CaCOL)
= 75 to U25%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO L)
I0 2q.
RQSA Ih f4+S H^()
I 100 132.
_%36 5.q 1 o 10 z 1 W° I qL l
I 362 +/-IjI Sl5.B l9 I051 099.V) ITYA ODE-o00 I I
l 1
lI.Z I q.tj I l'
(oi I 5~o liAct-o I
Ip.
I
~
_1-1 I
Iti
]
/*
T T
I 11 Ia 02 13.3.
W
- o I____
1_ 6 I
tog 5.B.Pw_
5 9
I Reviewed by:
Date reviewed: I to.3 0.OS l
I B
I I
I I
I I
I I
I I
I I
Page 6'
Page X__of Environmental Testing Solutions, Inc.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3lL Ir' s5t+a&
. 3 Analyst l1 II Z 1 u1'-o Date analyzed l ll OS Titrate samples to pH = 4.50 S.U.
Titrant normality and multiplier determination:
pH or Normality Normality (1N) of HzSO.,
pH Factor or Nlultiplier Deionized Titrant check Begin End Total
- (5 ml NazCO x 0.05)/E (Nx 50000)/100 ml sample water reference
'standard ml ml ml
- 0.251E
= N s 500
- 4.5 S.U.
number number (E)
(acceptable range - 0.018 - 0.022) ql 5 1
IA6R1s?
0.0 It.O O.
t20 IO'-
Laborato control standard:_
Reference True value Sample Alkalialty (MV)
% RS A1V / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3L)
(acceptable range (mg CaCOAIL)
(ml) ml ml ml
-90 to 110%)
-0R'e>
0 1 0loO 12.U
-21.
.q2 11.1 9 ts 91o0 Duplicate sam ple precision:.
Sample Alkalinity
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L)
((S -D) Il(S+Dy)/21 z 100 number (M
(l) ml ml ml (acceptable range
= 10%)
0-.tI.it l I h o-5g1 l0 S
1C.
J Duplicate 4
71.301 52 D
5C-MatrL' spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO,/L) l (mgCaCOCVL)
(ml) ml lml[
ml 1AA10e3 1 9v 1W 2bf.9 13Tq t1o-t' 10-4 W
Sample alkalinity (B)
Measured spike value (MV)
% R -
MV/ SV x 100 I
(mg CaCO)rL) l MV-"A -B (2cceptable range I
(mg CaCOIL) 75 to 125%)
I a
t
-o-I \\02t Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOILl JO351 oS/ A luI4b
- 42O 100 n3~L 41 o
.0 :.,0 i0.4 I
61 1
A1 S I 1_-
1 431o 46_1_1 1I d"
C Th+/-~s t
LQto 0.0 b.(C0 5.t S
i I-oS.sA M14S 1420 1 1..? 6A.il l
l) 1I.oS-as
£Q 4_ S___0 I
Iil Lo 1
1061031.0l1 -A-S4xK 1m I
OA
'23S (Pl.
/I 1102.12.
2 t?
O95;IIO2.l_
o 11 13412§X.
I 1/ lZQ 10510 31.o
-0?-SC>
Ar -IA jo wrb.
i23 F3 1
.0.
Reviewed by:
D ee\\e d Date reviewed: l1-1'113-OS7l
- 11.
I.. I.....
., i.
i nkofimental Testing Solutions, Inc.
Page s
Page
'_of l
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L
¶
- d: O'3 Analyst l Date analyzed 0
ni normality and rultiplier determinadton:
-i-1 rrv, &rICU-01. : 001:5 5-tre.:
I s.I.
I:.;...
I.:
...I,.
I.:.
.-I
- Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
-0.2/E
- (N x 50000)/ 50 ml sample number number (E)
(acceptable range - 0.018 -0.0t )
N% 1000
.i4llils IjJ 1 2
- 0. o q.0 ieQ Q.O2o1-2o.'A LaboratorY control standard:
Reference True value Sample Hardness (MV)
% RS - MV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOIL)
(acceptable range (mg CaCO3/L)
(ml) ml mnml
- 90 to Ito%)
S I40 50 f c i.so-Duplicate sam le precision:
Sample Hardness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L)
((S-D) ll(S+D)121) x 100 number (ml) ml ml ml (acceptable range - *t%)
1O-213o"5
,s 1_
_2
_S Duplicate I-13 2 -O 1
D 4.3
?.
Uatrix spike recovery:
Xeference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOIL)
I (mgCaCO3/L)
(ml) ml ml ml 40 6b 3.t1 li L.o A
e>
Z Sample hardness (B)
Measured spike value (MV)
% R -MV ISV x 100 I (mg CaCO3tL) lhV
- A - B I
(acceptable range (mg CaCO3/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg CaCO3/L)
T,
O.C..
, 0.0 l
S-J C1 (shouldbe i-mgCCO)l0l
° ;29,OSA l l-14149 i4o l 5 l
I i
I qI llo. Z4-os a 21.1 124 1Z 46l A
I 4.I I
2.
-Rf.4o i t
/Ak d -
Do JL13
,LJQ 6 I2 M
_ I
_ I_
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,1 Notc: If >I5ml of titrant is used, sample must be diluted.
Reviewed r
D Date reviewed I 10 -.30-0.5
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Date analyzed IIl.Dfo IJ Titrant normalitv anafd MnUaiftie def 1
Orma 1I - _-1 I - ---- __ _ _
I I- -
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Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3IL Titrate samples to pH = 4.50 S.U.
lefinn PtlOf Normality Normality (N) of H2SO, pH Factor or Multiplier Delonized Titraot chcc-Be E
_Xnd Total
- (5 ml NaICO3 x 0.05)1E
- (N x 50000) 100 ml sample water reference standard ml ml dit-0 25IE N x 500
- 4.5 S.U.
number number
=
(a=ceptabe Er)n Laboratory control standard:
Reference True value Sample_
Alkalinity (IV)
% RS - MV/TVx 100 standard number (m V) volame Begin End Total Multiplier (mg CaCO3 IL)
(acceptable range (mg CaCO/L)
(tl) ml ml ml
- 90 to 110%)
IAA et)'2) 100 tOQ 0v0) q.t qt
)le) of 13 1 Duplicate~samle precsion:
[
Sample Alkalinity
%R.PD -
Sample J Sample ID volume Begin End Total Multiplier (mg CaCO,/L)
I(S - D) II(S4-D)fll x 100 number 1A (ml) ml ml ml (acceptable range - *10%)
A Duplicate
-lq.
(
D I.to Mfatr&r spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV)
J volume Begin End Total l Multiplier (mg CaCOAL)
(m, CaCO,/L)
(ml) ml ml ml l
l I4 I83 IC) lo P5.
A(,SI l.oI 1 10 ICI Sample alkalinity (B)
Measured spike value (MV) l%
R - MV / SV x 100 (mg CaCO3/L) l MV A-B (acceptable range I
I (mg CaCOV/L)
-75 to 125%)
j Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOAL)
IS
_II 1213 IT4A_;,- 1,,r I
Io 1(;>
1fT NA 6.JI59 1 0w4
-W O6-1O O INA Ip 1 I I4A I
I A 1 21 1I IQ51b3. 03 2
I Zq._4 S
.01to.I l51OS.Oe 3 1 1
- 10.
4.S I
II l5110svi I 4 4.! ('40 l3 1 Oi5S l foS, 1
1__
_ 2 1.2..
I~
I2
- 2. __I2 8I A II 1 6_
Io,_
A II2IS5 3.2 l
_ _ _ _ __l l
QP\\DA.XH 1
Z, I
.1 I55.5 l
,- I I'
1 Reviewed by:
Dt rve c,
Date reviewed: I By. lo-0
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Page (O 3 Page of Analyst l 1Z 9-Date analyzed I I 20o -0:
l Titrant normalitv and multinnier dtterminatih Alkaliniiy (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U.
On
.- _-rw-Normalit Normality (iN) of H3SO4 pH Factor or I'viultiplier.
Deionized Tltrant cl-ee
- -Beis
-Ends Total
-(5 ml NajCOj x 0.05)/E
- (NV s 0000)/ 100 ml sample water reference standard ml ml mT _
0.25/E IV xS00 4.5 S.U
-number nulmber E
acptable a
n Labortolycontrol standard:
Rererence True value Sample I
Alkalinity (IXV)
% RS -
MNV/
TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOAL)
(acceptable range (mg CaCO IL)
(ml) ml ml ml
- 90to O10%)
1NS e3 100 100 1.j 10a6 Ci 3 1ID.9 9I C q Duplicate sam le precision:
Sample Alkalinity
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO 2IL)
((S - D) /I(S4-D)21) 100 oumber
_~I~
Ti
- ml _
_ml ml (acceptable range - t 10%)
05lotD'j WbtJ INmls 1 100:7 11 I
I.
S.
ji Duplicate 4
D I
g-qLi, D. -i-I l_.
Matrix spike recovery Reference Spike value Sample Spike alkalinIty (A) standard number (SV) volume.
1Begin End Total ultiplier (mgCaCOA)
(me CCOVL)
(mol) lml le ml ml l
l I
5 1O 1.6 NJ5 11.4 1.4 A
Z Sample Akalinity (B)
Measured spike value (MIlv)
% R MV / SV x 100 a(mpeCaCOVL)
MV-A - B (acCeptable range I
(mg CaCOIL) 7S to 125%)
I+
C I5 0,198 Sample measurements:.
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml I ml ml Multiplier l
(M.
L.
0 __
0 1SOS.lO T
ul!
(00 Aiq.S 139.0o S
lo.' -
6 6t)
- 4.
3 J
.4 I1 3.0 1 3 2 05 I I0S II o
Reviewed by:
I
-i Date reviewed: l
-1 3 -OS-
EnvironMental Testing Solutions, Inc.
Page
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Total Hardness (EPA Method 130.2)
Matrix: Water, MDL 1.0 mg CaCO3/L Analyst LI&III]
Date analyzed I I I e..o.t S l
TAMt S.
IIL
'r' rr ad-O '-
1155S Titrant normality and multiplier determination:
Tltrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
- 0.2/E
= (N x 50000)1 50 ml sample number number (E)
(acceptable range - 0.018. 0.022)
-=Vs 1000 114 Al 7S 1 WN Rlz O.
- 4.
2.9 _,
0.Z O 2-20.'
Laboratory control standard:
Reference True value Sample Hardness (WlV)
% RS - MV TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L)
(acceptable range (mg CaCOVL)
(ml) ml ml ml
- 90to 110%)
lJA55309 40 50 j A
1-2D ZA I 204.
'7
.o0.
Duplicate sam le precision:
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOCIL)
((S - D) /l(S+DYZIF x 100 number (ml) ml ml ml (acceptable range - ^ 10%)
10^3N-o4 bb
-1iX tD lo 02 X.
Duplicate D
14<3 4-D M at~ri spike reco ve j Reference Spike value Sample Spike hardness (A) standard umber (SV) volume Begin End Total Multiplier (mgC&CO,/L)
(mIg CaCO/L)
(ml)l ml lml ml m
la.
l (SS oq 40
-'i-
- .4 Z C22 1 0 Sample hardness (B)
Measured spike value (MV)
% R - MV I SV x 100 (mg CaCOIL)
MV - A - B (acceptable range (mg CaCOIL)
'6 75 to 125%)
Samlemeasuements:-
Sample volume Begin End Total Hardness Sample number Sample ID (mIl) l nml nl ml Multiplier (me CaCOL Blank 3 ID should bel-l0 mCaCO/L) 0.0 Q.O 0-.0)
Z -
bo Do.
ayb,,no Mn I-1H2.U
.51 '-1.
11.Oo;05 A 12s 32.-
L4.- I q_
1 nOs, T6 I
T W32 141.031lO q.Z5 VOs~a. o A__
4.l~
L__
111O5 O1.
1_
3.1-I
'JOI k
4Li.I I_
I
_I_
1OS I 1q.3 I4 11.0 I
05'b3l.
A 3
1 11oz u1
(
(Vj (0
INr Note: If >15ml of titrant is used, sample must be diluted.
Reviewed by:
Io -* l Date reviewed l I-13I-1
Page !,o Page 2
of3i!
Environmental Testin. Rnlutions. Inc.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst [im Date analyzed Lt I
i(04 TPIwzt norma14y and multiplier determination:
Titrant jorm gin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml-
- 0.21E (N 50A000Y 50 ml sample number number (E)
(acceptable range - 0.018 - 0.02-
- N s 1000 Laboratory control standard:
Reference True value Sample Hardness (MV)
% RS -
IV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO2IL)
(acceptable range (mg CaCO3L)
(ml) ml ml ml
-90 10 110%)
A J:5$
9 40 50 G
2.0 2oo2
-10o I)m Duplicate sanmple precision:
Sample Hardness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (m: CaCOi/L)
((S - D) 4I(S+D)/21) x 100 number 71 A (mi) ml ml ml (acceptable range -
t 10%)
binil LDb2ZZ.3 03N l Z 6.
204 Z!:L_ S j
Duplicate
- 4.
D I
-D Mat~rx spike iecoery:
Reference Spike value lSample l l
l llSpike hardness (A)l standard number (SV) volume Begin End Total Multiplier I(mg CaCO3/L)
(mg CaCO3/L)_l (ml) ml ml ml J
SS 440 5v
.24 2.4.1 5*Xf AO 20-1z 7-Sample hardness (B)
Measured spike value (MV)
% R - MV I SV x 100 (mg CaCOj1L)
MIV-A-B (acceptable range l
(mg CaCOL)
-75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (mI) ml ml ml Multiplier l
(mgsl~2L) l l
l
~~Blank i I
I Mg
( l-CaCO./L)
_____l l()5110-4.Db
-- r4ASQj,) ifil 3
l 4I-0O 2-t1-l 3}
QZ l Sll010) 4
,?
1lo 1 I I
I^.
.b eo I
'I Os 0lt6b
&3 I
Z 0
- 2.
NJ i.
1 54 sI Lf fl 7.
O* tEti-11-05 oscei.
3 36.6
'453-3 1} l~
g 1
4 051lot. O1.02-4A W OImI 1a wrl qI 4X 1
1 S
es O5 S
I 1c, 04 I
3-b Z.
e6 11._.
4.S I
_I 105X~o3.-
1§161tI l
1 9
Q51 1 tO1 03 Note: If >15ml ortitrant is used. sample must be diluted.
by:
Reviewed I
Ci l
Date reviewed It3-lOs I
Environmnental Testing Solutions, Inc.
Page 6 q Page 3
of3 Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst I ht)k-I Date analyzed 1I i G-6J Titratnt normalitv antd multinleir determination:
al_ c_
heck Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml
- 0.21E
- (.I x 50000y 50 ml sample number number (acceptable range - 0.018 - 0.022)
-N i xlO00 Laboratory control standard:
Reference True value Sample Hardness (MV)
% RS - MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L)
(acceptable range (mg CaCOi/L)
(mj) ml ml ml
- 90 to 110%)
t;5
@40 50 1
102.1 2.0 Z°2 24 L
[CO)
Duplicate sam le precision:
I Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)
((S - D) /l(S+DylP-I x 100 number A
I (ml) ml ml ml (acceptable range 10%)
of 10S-li' 1 3 3
l
.Z0.1 24q..
45
_,_L S
C1
-I-:.
4 Duplicate l l Z _
5 4
D 91
'Matrix spike recovery:
Reference Spike value S
Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOjIL)
(mg CaCOL) I(ml)
I ml ml
&S350
'JO0
- 60) 1 2tt-Wl-.1 b-.
20S 136 Sample hardness (B)
Measured spike value (MV)
% R YV I SV x 100 (mg CaCOL) l MV-A-B l
(acceptable range I
(mg CaCOfL)
_75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID J
ml ml ml Multiplier (m-CaCO3/L)
Blank Bill IL '1.0 ZL(4-kb.x e-t 4 'a I o,51o3
-,.g zT.3 4+/-c ______
l IoI
.i 3
I~
I~ I~
i_
I I
Notc: If >ISml of titrant is used. sample must be diluted.
by:
Reviewed l
%,J )
Date reviewed I w06-0s I
Sequoyah Nuclear Plant Biomonitoring November 1-8, 2005 Appendix D Reference Toxicant Test and Control Chart
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~-- '-~--
- --- Pmiep hales prom-elas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 0.8 I 0.7 0.6 0.5 1.2 USEPA Control Limits (I 2 Standard Deviations)
..............,,............................................................................................................... e,.,,,,...............
V
- N 1.0 0.8 0.6 USEPA Warning and Control Limits (75' and 9 0 h Percentile CVs)
_:::=_...........
0.4 I fl.
I
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0.8 0.7 0.6 0.5 nAo Laboratory Warning and Control Limits (IO'h and 2 5th Percentile CVs)
I V..T
- A =0 " -
ATs sot dat eS Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
- Warning Limits (mean IC25 4 SA.10 or SA75)
. Control Limits (mean IC25 +/- S A.25, SA.90, or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromdas using Moderately Hard Synthetic Water Trot smber Test dite 7dsyICY CT (gt KCI)
(at KO)I Stats wn4USEPA Laberstery LAboeatey S
CaimLndit SAM WaningLtndia SAM Cmiii Unlft SY,,
CT-2S CT+29 CT-SA.,
CT+SA,,
UsEPA USEPA Wandig Units BS
- CrlUMIts 1 CV Cr -SA.,
1 04-26-05 0.52 2
04-26-05 0.53 0.55 0.04 047 0.63 0.07 0.49 0.6i 0.12 0.44 0.67 0.21 0.34 0.76 0.25 0.30 0.80 10.07 3
05403-05 0.61 0.57 0.04 0.48 0.66 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35 0.79 0.26 0.31 0.33 0.03 4
05-10.05 0.5 0.57 0.04 0.50 0.65 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.36 0.79 0.26 0.32 0.33 0.06 5
05-17.05 0.50 0.56 0.04 0.47 0.65 0.07 0.49 0.63 0.12 0.44 0.68 0.21 0.35 0.77 0.25 0.31 0.81 0.0Q 6
06.07405 0.62 0.57 0.05 0.48 0.66 0.07 0.50
.64 0.12 0.45 0.69 0.22 0.35 0.79 0.26 0.31 0.33 0.01 7
06-21-05 0.61 0.58 0.04 0.49 0.66 o.07 0.51 0.64 0.12 0.45 0.70 0.22 0.36 0.79 0.26 0.32 0.83 0.08 a
07-12-05 0.53 0.57 0.04 0.48 0.66 0.07 050
.64 0.12 0.45 0.69 0.22 0.35 0.79 0.26 0.31 0.83 0.08 9
07-19.05 0.62 0.53 0.05 0.49 0.67 0.07 0.51 0.64 0.12 0.45 0.70 0.22 ONQ6 0.79 0.26 0.32 0.33 OO.
10 07-26.05 0.66 0.58 0.05 0.4B 0.63 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.31 0.26 0.32 0.85 0Qe9 11 0S49.05 0.61 0.59 0.05 A.9 0.63 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.31 0.26 0.32 0.35 0.08 12 08-16-05 0.52 0.58 0.05 0.43 06 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.30 0.26 0.32 0.34 0.09 13 0-24-05 0.63 0.58 0.05 0.49 0.68 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.31 0.26 0.32 0.85 0.08 14 08-27-05
.64 0.59 0.05 0.49 0.69 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.81 0.26 0.32 085 0.08 15 09-20-05 0.58 0.59 0.05 0.49 0.68 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.35 0.08 16 10t04.05 0.67 0.59 0.05 049 0.70 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.36 0.09 17 10-11-05 0.63 0.60 0.05 0.50 0.70 0.07 052 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.36 0.08 18 10-1S305 055 0.59 0.05 049 0.69 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.03 19 10-19-05 0.59 039 0.05 0.50 0.0 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.32 0.27 0.33 0.36 0.08 20 11-01-05 0.60 0.59 0.05 0.30 0.69 0.07 052 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.36 0.03 Note:
7d. IC2,
7-day 25% ihbition eomeatratiu. Aa estimstion ofthe concentirtion of potiam AbMiOeMth v l cause a 25% redacdtin Pincqhsles Uth oer the test popuhts CT -
4ten deny(mcm Iu).
S - Standard devimt ofthe BMn, bnbe laeratery Cents! awd WOrn U~ndts Labtoycetreand w g
swee esbc ned usigt m rd de in of tc C, vabes cocspcdngt tihe 10th and 25t pae tile CVL hee ranges re more tigent th the oet and wasig linkb recormended by USEPA for the test methd Bad eadpoitL.
3 A.Ie - Standard deviatin wmespwdg to dithe le peete CV. (SA.-
0.12)
=S
- Standard desiat corrspanding to the 2?a percentile CV. (S,, - 0.21)
USEPA Canr"l nai Wadnin Uifts A - Standard deviation amespondigto the 75e perce tile CV. (SAY - 0.38)
S.3*- Standard deyvi corspondingto the 90p percentile CV. (SAn - 0.45)
CV - Ceffinkent oftailation ofthe C,, Woes.
WSA 200. U p
_ s eotbrdVdilf hWbole
.ifhbdlbI fiWtX UdchNatblPohtant DEl kbsnPso EPA 433Rr50. UE t
_s my.
CbOH Orgastsw eo ltdsf Aq-leBlotSu e,
11v0 H.U140 1
--Environmental -Testing -Solutions,-Inc-.---- ---
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean CT CV CT MSD PMSD CT Survival Growth
(%)
(Ivae) for Control Growth
(%)
for Control
(%)
for PMSD (fo)
(mgllava)
Growth CV(%
1 04-26-05 100 0.742 3.9.
0.09 12.3 2
04-26-05 97.5 0.702 0.722 7.2 5.6 0.10 13.5 12.9 3
05-03-05 100 0.760 0.734 2.5 4.5 0.10 12.9 12.9 4
05-10-05 100 0.772 0.744 10.7 6.1 0.10 13.5 13.1 5
05-17-05 100 0.858 0.767 6.0 6.1 0.06 7.4 11.9 6
06-07-05 100 0.848 0.780 3.3 5.6 0.09
.10.1 11.6 7
06-21-05 97.5 0.622 0.758 7.2 5.8 0.09 14.5 12.0 8
07-12-05 100 0.765 0.759 5.6 5.8 0.10 13.4 12.2 9
07-19-05 100 0.596 0.740 2.9 5.5 0.06 9.7 11.9 10 07-26-05 100 0.807 0.747 11.5 6.1 0.11 13.2 12.0 11 08-09-05 100 0.622 0.736 5.5 6.0 0.11 17.0 12.5 12 08-16-05 100 0.599 0.724 i2.7 6.6 0.11 18.9 13.0 13 08-24-05 100 0.802 0.730 14.9 7.2 0.14 17.4 13.4 14 08-27-05 100 0.678 0.727 4.0 7.0 0.11 16.5 13.6 15 09-20-05 100 0.814 0.732 8.4 7.1 0.11 13.3 13.6 16 10-04-05 100 0.785 0.736 4.5 6.9 0.06 7.3 13.2 17 10-11-05 100 0.555 0.725 4.1 6.8 0.11 19.4 13.5 18 10-18-05 100 0.741 0.726 4.9 6.7 0.04 5.9 13.1 19 10-19-05 100 0.556 0.717 5.9 6.6 0.08 14.6 13.2 20 11-01-05 100 0.694 0.716 1.2 6.3 0.09 12.4 13.2 Noe.a CV-Coefficient of variation for control growth.
On average, the CV for control growth is 6.3% in Environmental Testihg Solutions, Inc. inmephales chronic toxicity tests Lower CV bound dtcrmined by USEPA (10' percentile) - 3.5%.
Upper CV bound determined by USEPA (90 percentile) - 20%
MSD - Minimum Significant Difference PMSD - Perent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mininum percent difference between the control and treatment that can be declard statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. clmnic toxicity tests when a toxicant reduces Pinephala growth by 13.2% from the control.
Lower PMSD bound detemid by USEPA (10 percentile) - 9.4%.
Upper PMSD boumd determined by USEPA (90* percentile) - 35%.
CT - Central Tndancy (mean Control Growth,.CV, or PMSD)
The lower and upperbounds were calculated by the USEPA using 205 tests conducted from 19 laoratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-O3. US Environmental Protection Agency, Cincimati OH.
Organisms obained from Aquatic BioSystems, Inc 1 1-01-03
Environmental Testing Solutions, Inc.
Pimephales promelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25 1.00
~ ~0.75 0.50
- 'E0.25 ----.--.
USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
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NZ3 30 Xl Kentucky Acceptance Limit (< 30.0%)
I- (
20 Q
°
~~.................................:;.-
~c 10 C
0o 40 30 USEPA Upper PMSD Bound (9 01h percentile < 35.0%)
20:
10
~~...................................................................
0 I'
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° bv A 60- S.I" (I 05I IO, Io O",, I 5
015 0s5 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD)
.Control Limits (mean Control Growth, CV, or PMSD E 2 Standard Deviations)
%iA Environmental Testing Solutions. Inc.
Page I of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number -S\\
ijI Dilution preparation infomation:
Comments:
KCI CHM number CAR4 lR8 Stock preparation:
50 g KCI/L: Dissolve 50 g KCI in I-L Deionized water Dilution prep (m&L) 300 450 600 750 900 Stock volume (mL) 6 9
12 15 18 Diluent volume (ML991988985 982 Total vohme (mL) 1000-1000 1000 1000 1000 Test organism information:
Test information:
Organism age:
21.15 rO 2i.ZS AWAS Oth Randomizing template:
G£ j Date and times organisms B-1o0s io iT t
Incubator number and were bom between:
shelf location:
3 Organism source:
AP e &
Pt tA-Artemia lot number.
6rANT Transfer bowl information:
pH =
SU Temperature -
°C Total drying time:
2ok f 1.1 24.Ci Date fTinie in: fio&t S
I23S Average transfer volume:
10 Date / Time out: A-M..
I1 I Oven temperature:
4 t
II A
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I Daflyfeeding and renewal information:
I Day Date Morning Afternoon Test initiation, MHS Analyst feeding feeding time renewal, or batch used time termination time 0
%%-ov-os Is.0 I 238 to z.-Uo6 6 C
1670 n'
I<<
OS 152.
1300)10-74os&
I ICBQ5 t YLei_
152L.
VZ to-,Ai-OSA I~-CAiC nP IoS0 L 7zo o o-S1-058 At It4A-tsc5 t
eSO tza
\\-US (A s
l* -c-6 I"_
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~
~
~
-s~j..
j 6
I.-M-0 hNS 14,L5L ZOO it-c&rC A, 7
1 I-08,-c Control information:
Acceptance critrca Summary of test endpoints:
% Mortalityv
- 07.
S20%
_ 7-day LCsp 1I Z. 1 Average weight per initial larvae:
- 0. C4'14
. NOEC A
Average weight per surviving larvae:
- 0. IOcq 2 0.25 mnglarvac LOEC l( 000 ChV IC25 i
-1 6DM0.
3
ronental Testing Solutions, Inc.
,SSpCies:
Pimephalespromelas
- ' c- **'
Page 2 of 5
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- - -.-...1..1- __......
PpKCICR Test Number.
8\\
Survival and Growh Data I -
I I
I I
Day Control 300 m KCI/L 450 m KCIUL A
B C
D E
F G
H I
J K
L
_I 10o
/0 10 I
/
O
/O
/
O tO'O
/O O
/0 0O 10 /0 /O lo /O /0
/O O /O O
3t JO 10
/0 /O /0 _
/O /0 /_ /0
/O /0 1 0 /t
/0 /D JD lo
/0 /0 1 I 1t ID 10 s
/D1 0
/0o /o
/ot o0 /O /c o /o /D 6
tot o
o 10 t0 10 J1 10 1, 0 4
7 1t ID0 10O1 to O
0 to io I
0
/O 9 A - Pan weight (mg)
Tray color coded:
l IS4*
14.5to is./ilqA1e 1
qfL4 r,.pL.
I'l
- 1 P43 %
Ie.lO Analyst:'2 a
e.i'~'
114.8 l1 B - Pan + Larvae weight (mg)
Zz
\\Z~ l S5 x z
i.24 3 ZI.f.ZB zj.6' X1 13 2zFq 1CL 8 O9 2
l Analyst:
-a Larvae weight (mg) - A-B
_Z C_
4e11 @
rXiu 1.0 C XI &
510 17.1 1 1.13 (D-7 3tr2A 1.10 D s A3 O
§t Weight per Initial number of larvae (mg)
- C / Initial number of larvae 9.
O'
[I O'
%O
.1%I AID
'2 ft
.0
'^V 0*
0 0*
%S NO
,Z ID' Average Percent weight per reduction Initial from control 0.(-L number of
(%)
larvae (mg)
I -
I -
h C
.5 7.
0.ID11 3.41.
i a
Comment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.
Calculations and data reviewed b Comments:
I
- Environmental Testing Solutions, Inc.
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Page 3 of 5 Species: Pimephalespromelas PpKCICR Test Number.
& X I
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Comment codes: c = clear, d - dead, fg = fungus, k - killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r - decanted and returned, w = wounded.
Calculations and data reviewed:A I
Comments:
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA4s21-R-02-013, Method 1000.0)
Species: Plimephaespremefas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses T_ _rmben PpKCR 1l21 (612 S351 D0epot dSI)
Tat deem Newrf *143, 2"5 Rewered by:
_1-T I
Uns C__mf AWBO b#Mb~~
F_
__ b61 A-ft oxn 4.'
R b~
~am larm A
A 10 10
-C 10 10 a
10 t0 F
10 10 C
10 10 K
10 10 9
10 1 to G
to o
10 9
4.4 to 10 O
10 9
o to r
10 7
10 3
7S0 R
10 IS S
to 3
T 10 7
-W 0
2_
X 10 O
W 10 I
x I
10 0
ii_
16.12 14.61 14.78 14.56 16.10 14.46 IS."
2244 21.12 21.61 20.92 20.01 21.57 21.68 11.71 17.12 19.86 11.54 19.78 CA-1 6.94 6.90 7.06 6.67 5.70 7.11 7.13 632 7.21 7.10 6.36 6.16 5.55 5.58 5.44 4.31 2.0 3.96 3.94 4.79 WVtlt Id uI Csdte.w,'5. ft Wa9h*WwIIj M
_O m-larE
-t-I
§-
d I
I............
I....................................
_W lA bllC e#_
S 0.694 0.694 1.2 0.690 l
1 A"
Not a12 able 0.706 0.687 0.570 0.657 10.5 0713 us7 10S 5A.4 0.713
~~ ~~0.632 0.721 o.6 s.s 0.710 j7W 7J 34 0.636 0.616 0.555 0.611 8.3 05*
Oin 11.7 24J 0.544 0.431 0.208 0.741 8.2 0&396 st one 31.6 46.7 0.479 I
%)
]
Wd 4%)
0.620 0.660 0.616 0.693 0.792 0.796 0.684 0.620 1.110 0.470 0.733 45.6 0.124 0.111 0.047 0.000 Sil 62.8 "A
Doweelt' MSD3 val PMSMh 124 MSD-MinloaSlwiffciutDiferee PUSD-Poort UMini six fimOlferce PMSD ismeswoepeecwi The 7PMSD is the m prt diffmebtweecthenb canOud tr0 Ih e be decred fs clyunsc ain awholecmt toxkitytsL On avnMusigicucdiffn coree sfor Eo i or t
Tert Solro.c.
etmuic loxicity tests when atoxkiant reducP e pw
_eby 132%
tmim (detabeIthro re. txklpt ks5eti Lower FMSD bimld detamnl by USEPA (loth pacenuik) - 94V.
Upper PMSD htnd *derminedl by USEPA (90th peieekle) - 359.
The 1,ed -
e-t
- r h.lnmi e USEPA -.
205 tel cmducted km 19 Iahntarie for ?iumekl b
rowlh in denie refamm tmtleft USPA 2000.UndOulnsdhcnmuigrormotrhedVeWlittyiWholen AKToxiiyApplicstim tindereNtionlulltDicheeElion Pra nEPA433.It-003. US avtnua otecAgacy.Cincmo.OIL Olmbws oobw dfi-At-dBio$yia-hs.
1 05
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- Environmental Testing Solutions, Inc......
Statistical Analyses 1MVa1 FAd CGrowth md Survival Tt-7 DMY Grow Slt nDate 1111Js Ttd D.
PpKCXR Smpsk ID, RE14taffoIlant Bod DatD Iw/2c05 LAb D.
0--v ITesingSL Sampkk I7pw KCaPobukm dii1e SampIe Date:
?atcok FWCHRAEPA 521-R-024U0 Tedl Spes:
P Pikph 5s re a
COUMrl:
ConeaiWL 1
2 3
4 D.Contol 0.6940 06900 0.7060 0.6170 300 0.5700 0.7110 0.7130 0.6320 430 M7210 0.7100 0.6360 06160 600 0.5550 0.5510 0.5440 4310 750 0.200 0.3960 0.3910 04790 900 0.1240 0.1110 0470 0O.OD Transform Uataffunded I-Tawd bowak C-s/g/L Ma NMe M
Ml Mr CV N
Sat Ical MSD Mom N.Mea D-contol 0.6943
.0000 0.6943 0.6170 0.7060 1.202 4
0.6943 1.0000 300 0.6565 0n4M6 0.6565 057 0.7130 10.496 4
L.W0 2.290 0.0860 0.6636 0.9e59 450 0.6708 0661 0.6701 0.6160 0.7210 7.121 4
0.626 2.290 0860 06636 s9559
- 60D0 0.5220 e
.59 0.5220 0.4310 0.5510 11.679 4
4.515 2.290 0.0160 0.3220 0.7519 750 3703 0.5333 0.3703 0.200 0.4790 31.025 4
03703 0.5333 900 n0705 0.1015 0.070s 0.0000 0.1240 81.996 4
0.0705 0.o1s AahlgaryTeaft Sted&
aik Wd Skew Kert 9 pko-W91 TO atbds s dhstrsun > 0.01) 0.911339193 0.8"4 0.70822108 446093538 BRlieft Toa Judintes eqratva(own O f
0.05) 7.722052574 11344675 Hyptheab Tat (Ctb1.4.l.05)
NOEC LOIIC OV TU MSDa MSDP MSB ME F-Pre&
df Daatca Tet 450 600 519.6152423 0.06033244 0612392257 0.02402417 0.0022275 0.002669202 3.12 Teemnrt ve D-ontrol Lker Iateraloit (200 Hempisa) paoit
.,/L SD 95% alA xp)
Skew I=05 454.33 115.00 0o.
49273
.4417 ICIO 491.09 34.09 423.33 53192 4.1418 IC15 527.86 1.07 466.90 575.11 42315 1.0 IC20 S64.62 22%U 500.05 623 42 4.1223 1C25 60130 23.32 519.54 66095 03101 IC40 704.
33.91 60t.03 791.05 41302 0.3 IC50 761.37 26.97 649.82 102.50
.0.499 0.7 10.8 0.3 0.2 0.1 0.0 0
200 400 6oo 800 1000 Doe mgL Desa,1espone. Plat 14ll, 0.06 WWIl 0 xlgntcance I
I O
wanio-obtainedfrom Aquatic BibSyst.
Infre 1 1401-5
Environmental Testing Solutions, Inc.
Page 4 o~f 5 Environmental Testing Solutions, Inc.
Page 4 of 5 Species: Pimephalespromelas PpKCICR Test Number:
8 l Daily Chemistrw:
I Dav I
I Analyst I Concentration Parameter CONTROL pH (S.U.)
DO (mrIL)
Conductivity (amhos/cm)
Alkalinity (mg CaCOt/L) 4 Hardness (mg CaCOS/L)
\\--D Z _
Temperature (0C)
II I I
I
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I pH (S.U.)
- 1. 1 I
DO (mz/L) 300 mg KCI/L, Conductivity I(I"mhos/cm)
V- 0 9.0 193S 7S.o Temperature (0C)
.4-.5 24.S I
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b -
dl I.
pH (S.U.)
'2.
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450 mg KCI/L DO (mg/L) n.i0d i
Conductivity
(;Lmos/=)
// 0
/S30 2S. 0 II I
E r
g f
Temperature
(°C) 2q.1 I
2L4.S I I zq. a I Zs. I U.
-~
-S0 I -
.3f l -
I?+/-2I DH (S.U.)
l 600 mg KCI/L DO (mg/L)
- 2. IL Conductivity (guhos/cm)
./3 o
I I
z.
ZS I I
Temperature (0C) 2l.
Z4-.7 I
/ 350 7..
zs.j 1 4.s f 7
-Y I -
pH (S.U.)
I
\\_
,4
-L DO (mg/L) 750 mg KCI/L Conductivity(<,
(gmhos/cm)
V 8.0
/1,20 Zs. I aI Temperature (0C)
IIZqI.4 I
~
dla IL-,zq. I 50 2.q (
w m5q pH (S.U.)
II w4. W I
900 mg KCI/L DO (mg(L)
Conductivity7 Temperature c1C)
'2.
¢ 1420 Zl 2S. I
-1/f5°,
_Final Initial STOCK Conductivity I (pimhos/cm) 17-2 0
I
/110-WW7 11 F
ina Initial 2q. q Initial
I Environmental Testing Solutions, Inc.
Page 5 of 5 I
Species: Pimephalespromelas PpKCICR Test Number:
9 I UI Day
[
I 3
I 4 -rM I m 4-Concentration CONTROL H vks I
I I
I
.1 0
N I
a pH (S.U.)
4-11 Q.352 I r4.X)- I 7.9-I W. /
I
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-1 t-%
9 t
U.1;;
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¶ I UV (MWL) a 300 mg KCI/L Conductivity (gjmhoslcm)
- -I 533-I K -7 I-4f.
V ~>
I Temperature (0C) 2L4.A I
Z4S.
I
- 9.
Ii).
DH (S.U.)
%4. 1 -t7 1./o
}. 7 450 mg KCUL DO (mg/L)
Conductivity (Amhos/cm) v Temperature (0C IN A I N'0 24.1 l1.-o 11.0 lTAZ N
I
//'/O I -1.43' I
vt.3 I I.'4A 2q-q
- 1 I
.g.
- -
ii -
pH (S.U.)
- I.
II I
Ton I
'6./0
+.Th
§ v
ll A-if L
LL....g 600 mg KCI/L DO (mg/L)
U I
Conductivity I
(gmhos/cm)
/356 13eo I
I16 13ezo V4.
I's
/380 Z,.,p Temperature (OC)
I I Z4.1 I' I
I
-q.'4 I I
9 S
i pH (S.U.)
II
. -.6L5 I.Z
- 19. i n I DO (mg/L)
I 750 mg KCI/L Conductivity i'
(pjmhos/cm)
/1 Z° 127 2t1.07 K
I 1.,
JlotaO I
I
/43 4
Zq.t 243 Temperature rC) 214.
I I
I 2z4.3 I pH (S.t.)
ell 2 DO (mg/L)
- 1 G.
900 mg KCiJL Conductivity (Amhos/cm)
I Temperature
- 24. 8 (0C)
STOCK Conductivity (mnhoslcm) 1 3/1 7 a
MI I
dl-- -
'"-ep I I
I
- ty 97 I 8w- 011
- w/
- 1.
I 1 -44 1.S1 1q30
-~
7-1.31 2q,7 [24. 1 I
I ID -'
I
_ _'U'_'
Xq n 261
-7o 6.
Initial l
IL zqJ. 7 Initial I Initial
_1 I
r Mal I
I tH r4 cra
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 1.12 I
I I
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I IeIaIoI USEPA Control Limits (d: 2 Standard Deviations) 1.10 I-1.08 I-1.06 1.04 2.5 I
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~0-2.0 1.5, USEPA Warning and Control Limits (75h and 90'h Percentile CVs)
S 0
~
S 1.0 0.5 1.4 1.3 1.2 1.1 1.0 0.9 A R Laboratory Warning and Control Limits (l and 2 5'h Percentile CVs)
Ll t
v.v Test date 7-day IC2. = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
- Warning Limits (mean IC25 + SA.I or SA, 75)
.Control Limits (mean 025
.25 SA.90' or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toticant Control Chart for Ceriodaphnia dubla using Moderately Hard Synthetic Water Test nunijer Text date 7-day ICI$
CT (g/LNOC!)
(giLN&CI)
State BW USMA S
Centra l Untb SA.
CT-28 CT+2S Laborateuy Waning Ltnit CT -SAJs Cr + 8S, Labuatery SAMS Central Lnits
- SIAS CT - SAIS CT + SAM5 USEPA Wanfn Lhnlts CT - SAM CT + SASS USIA SA.
ContrLtnift CT - S.,
Cr + SAM Cv 1
2 3
4 5
6 7
8 9
10 11 12 13 14 is 16 17 18 19
'20 06-0804 07-07-04 08-10-04 09-14404 09-14-04 10-05304 114204 12407-04 01-11-05 02-04-05 03-01-05 04-05-05 05-03405 0647-05 07-12405 07-12405 08t905 09-13405 10-0405 11401405 1.06 1.06 1.06 1.05 1.07 1.07 1.06 l.OS 1.06 1.05 1.08 1.09 1.07 1.05 1.06 1.06 1.08 1.04 1.09 1.08 1.06 0.00 1.05 1.06 0.08 1.06 0.00 1.05 1.06 0.08 1.06 0.00 1.05 1.06 0.08 1.06 0.01 1.04 1.07 0.0O 1.06 0.01 1.04 1.08 0.0O 1.06 0.01 1.04 1.08 0.08 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04
.OS 0.08 1.06 0.01 1.04 I.OS 0.08 1.06 0.01 1.04
.09 0.09 1.07 0.01 1.04 1.09 0.09 1.07 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 1.07 0.01 1.04 1.09 0.09 0.97 0.97 0.97 0.97 0.9S 0.98 0.98 0.98
.0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 0.98 1.14 L14 1.14 1.14 1.15 1.14 1.15 1.15 1.15 1.15 1.15 IL5 1.15 1.15 1.15 1.15 1.15 1.15 1.15 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.IS 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.1S 0.18 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.88 1.24 0.8S 1.25 0.88 1.25 0.88 1.25 0.88 1.24 0.88 1.24 0.88 1.25 0.88 1.24 0.88 1.25 0.88 1.25 0.48 0.4S 0.48 0.48 0.48 0.48 0.4S 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.4S 0.48 0.48 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.58 0.53 0.58 0.59 0.59 0.59 0.59 0.59 0.59 0.58 0.59 0.59 1.53 1.53 1.53 1.53 1.54 1.54 1.54 1.54
.1.54 1.54 1.54 1.54 1.54 1.54 1.54 154 1.54 1.54 1.54 0.66 0.40 0.66 0.40 0.65 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 1.71 0.00 1.71 0.00 1.71 0.00 171 0.01 172 0.01 L72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.73 0.01 L73 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.73 0.01 Note:
7-d IC" day 25% inhibition oonetha An estintion ofthe concentration ofsodium cluode that would cause a 25%Z seduction in Cerodaphafo spoducion for do t popuadon.
CT - Control tendeney (men IC 2 )
S - Standaid deviation of the VC21 shle Laborutery C*nts, and Waning LUnits Laboaosy contmls ed w ing lswo ctebliu wusig the sandard ddeaon of th IC2 vlu onepding to the 10t and 25thhpcnnbtle CVe Thse isuge1 *la e
zn t
{tn the cont1 mt dwarning Niits a
ontm ded by USMPA for tli test W
iod and ondpoint
-L
- Standaid deviation ce ponding to the 0* pertceilo CV. (SAJ. - 0.08)
SAm - Standard deviation coueponding to he 25'6erecntfle CV. (SAS, -0.17)
USErA Contral ad Wanng Unfti SAss - Standard deviation coneponding to the 75*peeeasble CV. (SAi - 045) 8 A
- Standard deviation coneronding to the 90'percentile CV. (SA. -0.62)
CV - Coefficient ofanticion of the IC2 valoea.
USEPA. 2000. Undeanding nd Accouning fofMctmd Vabiality in Whole EutToxicity ApplicationsUndet timNational Pollutant Dinsge Eaimination Pregam EPA.833-R-00-003. US ninmnal PFtction Agey Cinca, OIL 14145
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water est TControl Control Mean numaber Tst date Survival Reproduction for Cc (ff.)
(offspringffemale)
Rep (offspr CT CV CT MSD PMSD CT for Control
(%)
Reproduction CV (V.)
(/)
forPM8D(%)
1 06-08-04 100 2
07-07.04 100 3
08-10-04 100 4
09-14-04 100 5
09-14-04 100 6
10-05-04 100 7
11-02-04 100 8
12-07-04 100 9
01-11-05 100 10 02-08-05 100 11 03-01-05 100 12 04-05-05 100 13 05-03-05 100 14 06-07-05 100 1S 07-12-O5 100 16 07-12-05 100 17 08-09-05 100 18 09-13-05 100 19 10-04-05 100 20 11-01-05 100 32.9 33.3 27.4 28.7 28.8 29.6 30.5 31.8 31.0 30.0 29.9 32.4 29.9 30.4 30.4 31.1 28.3 27.9 27.0 28.4 33.1 31.2 30.6 30.2 30.1 30.2 30.4 30.4 30.4 30.4 30.5 30.5 30.5 30.5 30.5 30.4 30.2 30.1 30.0 6.8 6.3 5.8 4.9 7.5 8.2 8.5 4.9 9.4 6.8 6.6 6.9 7.0 5.0 7.5 7.2 7.3 7.3 5.8 10.3 3.0 9.0 6.6 2.6 7.8 6.3 2.3 8.6 6.0 2.4 8.2 6.3 3.0 10.5 6.6 2.8 9.6 6.8 2.7 8.8 6.6 2.5 7.9 6.9 3.1 10.0 6.9 2.4 8.0 6.9 2.9 9.9 6.9 3.1 9.6 6.9 2.9 9.8 6.7 2.6 8.5 6.8 2.7 8.8 6.8 3.2 10.2 6.8 2.9 10.3 6.9 3.9 13.9 6.8 3.0 11.1 7.0 3.8 13.2 8.4 8.4 8.4 8.8 8.9 8.9 8.8 8.9 8.8 8.9 9.0 9.1 9.0 9.0 9.1 9.2 9.4 9.5 9.7 Note:
CV - Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 7.0% in Enviroumental Testing Solutions, Ine. Cariodaphnia chronic toxicity tests.
LowerCV bound deterinedbyUSEPA (10' percentile) - S.9..
Upper CV bound deterninedbyUSEPA (90' percentile) - 42%
MSD - MinimunSigificantDiffcrence PMSD - PerntinimumSignficantDifference PMSD isa nuasure oftest precision. The PMSD is the mnimumperent diffnce between the control nd treatment that can be declared statistically significant in a whole effluent toxidcity test On average, a significant diffirence occurs for Environmental Testing Solutions, Inc= chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.7% from the controL Lower PMSD bound detannined by USEPA (10 percentile) - 11%.
Upper PMSD bound determnined by USEPA (90' percentile) - 37%.
CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted fromn33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA 2000. Understanuding nd Accoundng forMethodVariabiityin Whole Effluent ToxidciyApplications Under the National Pollutant Disharge Elimination Program. EPA-833-R00403. US Envirornental Protection Agency, Cincinnati, OH.
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests C
S-iS C6~
4 s) 40 35 30 25 20 15
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Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
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= 10
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North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
E
)
40 1
100"K i
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USEPA Upper PMSD Bound (90e percentile < 37.0%)
I
~~~...............
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IL Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD - 2 Standard Deviations)
Environmental Testing Solutions, Inc.
i :'
Page I of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCLCR #:
4 ?-
Dilution preparation information:
Comments:
NaCI CHM number:
CR.
Io Stock prepamtion 1001 NaCIII (dissolve 50 g NaCI in 500 ml deionized water)
Dilution prep (mg/L) 600 o800o 1000 1200 1400 Stockvolume mL) 9 12 1 15 I i 21 Diluent volume (mL) 1491 148 l
14S5 1482 1479 Total volume (mL).
1500 1500 1500 1500 1500 Test organism information:
Test information:
Organism age:
< 24-hours old Randomizing template:
W
-LLo Date and times organisms II-oW-cs5 a'Wt 'r Io Incubator number anda were born between:
shelf location:
r
^-'
Organism source:
IbD-2S-e Ap-F YCT batch:
____os Transfer bowl information:
pH 1.91 SU Temperature ZqA'C Selenastrum batch: -
o-g 5
Daily renewal information:
Day Date Test Initiation, MHS water Analyst renewal, or batch used termInatIon time 0
_t_-O__s
.110 t
-24FOs
._I l\\02-0s ltODLo 10 24 0S 6 A4 2
lA-6! 05 Oc)L.
l-31 A
d\\
I3c&OC 00a eo lo*t-os I Zt2
. ~
~
~
~
4 2Mcs-o S0C-82hb
~
t 6 _
_-_Os 1c005 ll S-1 A A\\
7
-o-o I o:
Control information:
Acceptance criteria Summary of test endpoints:
%ofMaleAdults:
20%
7dayLC50
')
i4 oC)
% Adults having 3 ' Broods:
100%
2 *o0 NOEC
% Mortality:
0
_5.0 s20%
LOEC 1
Mean Offspring/Female:
2&
2 15.0 ofpring/fentale ChV lOR54
%CV:
IoD 57 c 40.0 %
IC25 l 1S.O
E m
T S
I Environmental Testing Solutions, Inc.
Page 2 of 6 I
I..
i Species: Ceriodaphnia dubia CONTROL CdNaCLCR #:
I 2-Survival and Reproduction Data I.
I I
I I
Replicate number Day 13 4
Sf6 7
8 9 r 10 I
Young produced OQ i
1 l 0 C ) l0 Adult mortality t _ [_
I L l
I l
> l 2
Young producedtOOlOOC Adult mortality z
i 3
Young produced O
f 0
O O
O l l
Adult mortallty
%.5_l-
_ -lll 4
Young produced
')
01 _ _
0 0
0 A
Adult mortality 4L 4 L.
'*l S
Young produced I
O I
Z2 I C) 1 Z _
I I CO. ]Ii Adult inortality I \\I-
'1-6 Young produced O
l f
OJl0Cl Adult mortality
- I l1-7 lYoungproduced IL l
4 1
2 Zl3 Total young produced 3
l 2kl 30 2:5
^3o lS z9 l
Il Z 2 Final Adult Mortality L-I-
I C?
l L-X for3' Broods I
r Note: Adult mortality (L - live. D - dead) 4-SPuTr 6Roo Concentradon:
- %Mortality:I Mean OffsprinnFemale:
7A. 'l 600 mg NaCI/L Survival and Reproduction Data
[
I Replicate number Day 12 3
4 5
6 7
8 9
10 I
Young produced C_
Adult mortality t
I L
L 1..
2 Young produced C
p
)
1 Adulttmortallty l
2 lI
- 1L Adult mortality 2
4 Young produced
'Al'
-A
=
Adult mortality l l s-L V
Ll SlYoung produced
- 12.
I I
i IC) i 1
/
1 Adult mortality l
j t? l L[CT l-tI Tl Young produced c
-3 1 i 1 0 0 I O
O O
Adult mortality 7
Young produced l.
I 5
1.
i i -
5 1
L Total young produced z
A 3
2
~
3
~
Q 1 3 Final Adult Mortality U.
iL C ?T T
Note: Adult monality (L - live, D - dead) tSattT 61=b I Concentration:
I%Mortality:
0 O
l Mean Offspring/Female:
I 2-q-Z
% Reduction from Control: l - 2. 1
Environmental Testing Solutions, Inc.
Page 3 of 6 Environmental Testin2 Solutions, Inc.
Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:
4_ _
800 mg NaCI/L Survival and Reproduction Data I
I.
.i i
Repieate namber Dat 1
2 13 4
6 7
r lO I
Young produced C):
O Cumber Adult mortality l
L.
L..
L.
2 Young produced O
=
Adult mortality V
L L-l t _-
l 1 L l
3 Young produced j
C)
O l
0O C 0 l O Adult mortality
%- I tL I Young produced l
4 l
=
lA 3l Adult mortality l
Q 2l L
Young produced I
tI j
O 1
j 1
I i
Adult mortality L.
LtL II-L-
l.
6 Young produced lO lO *O C)
W O
o loIo Aduk mortal-ty l
L -
ta =
a n.-
-I 7
oYung produced 1
l l
IL, o l l
I Z
l12 Total young produced l 2l l
Cj T l
I l
I T
T Z
2 Final Adult Mortalty J
L lc r
L.
l
(
Note: Adult mortality (L - live, D-dead) st S
r
'b
- IConcentration:
%Mortality:
o r l Mean OffspringlFemale:
I 21Ai 1
% Reduction from Control: l1.
t7.
j 1000 mg NaCI/L Survival and Reproduction Data Repllcate number Day I
2
..3 4
1 5
6 7
S 9
10 II I
Young produced IC) 0 0
I O
=
O lOlOiOl Adult mortality t
I L
U L.-
l2 lYoung produced l()
0 Q
- l Adultmorta~lty 0
L l
l3 lYoung produced l C) l Cl(
OlOlC
@C C
)
C )I Adult mortality
_I-L-
4 Young produced z
U s
Young produce c)
It morta1 tyI L-L 6
Youngproduced
'l O
l3 O i
O l
0 03 Adult mortality l ll L 7--
k l
T L-6 Young producedtL1 IQJ
(
( O I
I o t a 2 I
d l m o t l.
U. j 1......
U.
. J 7 C Totalyoungproduced lZ l31 l z-lq 29 lZ3
- 23. Z3 21 lL4 Final Adult Mortalitv
[I F
l a
t
-1 Note: Adult mortality (L - live, D a dead) 65fLIT 6"i 1:
l Concentration:
% Mortality:
l:I i Mean OffspringFemale:
I 2Zo.1 1
% Reduction from Control:
I. C)% I
r Ik -:
j..V t
II I
II i.I I
Environmental Testing Solutions, Inc.
Page 4 of 6 Species: Ceriodaphnia dubsa CdNaCLCR #:. A 2.
1200 me NaCl/L Survival and Reproduction Data I
I I
i Replicate number I
Day I_
2_
3 4
1 S 7
It 9
1 1 I
Young produced I
O O Adult mortality
_ I L
L L
2 Young produced O~
.l _
c Q
_-mortality [Ii I I L I I L 3
lYoung produced O
o O
O:
Ol01 0l0 l
AIult mort*li ty.
t[-
0
+
c-R I l l2 l V
e -
4 Young produced 4
Z_
l_
l Adult mortalilty 5
Young produced lD i
5 8 l S l
S l 8 Adult mortaliqty L8LlLl 6
l Yong produced C)
O)l O
O C3 O
l Adult mortality U-L-
. I -
L L-.--.__
J__
-L-L -
6 Young produced 13 I
O Total young produced l
z it 2(
ll1 n
l1 0
15 l ot Final Adult Mort mlity
[
l L
Ll 1-Ll
.1 Note: Adultmoality (L-live, D - dea) 7 Y g pConcentration:
n %Mortaliy:
U..
l I Mean Offspring/Female:
i s3. i
% Reduction from Control:
55.'
S 1400 mg NaCllL Survival and Reproduction Data Replicate number Dav 1
2 13 4
IS 6 r7 8
9 t0 I
Youngproduced C) 0 7 0 10100 O
O I
1 Adult mortality l Ll L
L --
L-L-
\\.
L -l L-2 Young produoed j i
O7 i
C)
O l Adult mortality
(
L a
a a
L a
(
3 wYoung produced Clr C)I
-°l Adult mortality l
1 L.
-l 4 I
1 L I 4
Young produced
'-4 L 0
- 2.
c._
0 C
Adult mortality l
U ll r
U I
U U
U U-S Young produced ILA
-i 6
s 9
2L..ro Adult mortality
[
U U
U.
U U
Young produced Q l 0
C I
Cn Adult mortality L
ll L
-J T*
7 Young produced
.5 5
Cj
( I 0
S Totalyoung produced I
lL I Z 1
1 l t Final Adult Mortality I
I I
U..
- w c
-c I
Note: Adult mortality (L live, D dead) l Concentration:
% Mortality:
7 I
I Mean Offspring/Female:
l 95
% Reduction from Control: 1 ".
1
Environmental Testing Solutions, Inc.
Verification of Ceriodaphnia Reproduction Totals Control Day
~Re be~ate number Tota Day 3
4 5
6 17 8
Total1 I
0 0
1 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
4 4
4 3
4 3
4 3
4 37 5
0 12 10 12 10 2
0 11 0
10 6
6 13 0
0 0
0 12 11 0
H1 0
4 7
14 is 12 14 12 1
U 13 1
3 Total 31 34 261 30 25 30 25 29 27 2
8 Day
~~Re placate numberToa Da 3T~ 4 5 16 T1i7 T 8
W9Tota I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
4 3
4 4
3 5
40 5
12 11 1
11 11 10 10 10 10 12 9
6 0 10 131 0 0
0 0
0 0
0 1
7 12 13 15 12 14 17 12 15 15 16 14 Total 28 291 33 27 29 30 26 29 28 133 29 800 mig NaC1/L~
Day
~~Re rbeat. number oa Da 1j 2j-3 4
5 6
7 8
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
3 3
4 4
4 4
39 S
12 14 10 11 10 12 9
10 0
11 99 6
0 0
0 1
0 0
0 0
10 0
11 7
13 111 13 It 16 14 14 12 14 12 130 Total 29 130 27 27 29 129 127 26 2
27 1 279 1000 mg NaCIIM DyReplcate number Total Day T 3T 1 4T 6
8 9
1 0 O
2 0
0 0
0 0--
0 0
0 0
0 0
3 00 0
0 0
0 0
0 0
0 0
4 4
4 3
3 4
3 4
4 4
3 36 5
1 13 0
12 0
9 9
0 10 9
63 6
10 0
10 0
1 0
0 9
0 0
40 7
17 14 13 14 14 11 10 10 13 12 128 Total 32 31 26 29 29 23 23 23
.27 2
6 1200 mg NaCI/L DayReplcate number Total 1
13 4
5 7
8 9
10 0
2 0
0 0
0 0
0 0
0 0 10 0
3 0
- 0.
0 0
0 0
0 0
0 0
0 4
4 4
3 1
2 5
2 2
1 3
27 5
5 5
8 6
5 8
4 0
6 55 6
0 4
0 0
0 0
0 3
0 0
7 7
13 1 1 012 0
0 0
0 60 4
Total 22 24 11 19 7
13+
6 5
59 13 1400 mg NaCI/
Day Replicate number Total 1
3 4
7 8
9 10 I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 0
2 0
0 3
3 0
0 1
13 5
2 3
7 3
4 4
5 9
4 0
41 6
0 0
0 0
0 0
0 0
0 7
7 7
1 5
5 9
3 0
16 0
5 0
34 Total 7
8 14 12 7
7 114 9
9 8
95
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnla dubfa Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
CdNaCICR #71 (#42 at 351 Depot St)
Teat dstes:
November 01.08, 2005 Revelted by:
_/44___
Concentratlon R pli-at e number Survival Average reproduction Csle.4 Peru" nd h
(mgAL NaCQ 1
2 3
4 5
6 7
9 10
(%)
(offsprlngtemale) v"'(
")
go he Control 31 34 26 30 25 30 25 29 27 27 100 28.4 10.3 Not appiteable 600 28 29 33 27 29 30 26 29 22 33 100 29.2 7.9
-2.a a00 29 30 27 27 29 29 27 26 28 27 100 27.9 4.6 1.S 10"0 32 31 26 29 29 23 23 23 27 24 100 26.7 12.9 6.0 1200 22 24 1 1 19 7
13 6
5 15 9
100 13.1 516 53.9 1400 7
8 14 12 7
7 14 9
9 8
1I0 9.5 29.5 665 Dunnett's MSD value:
3.751 PMSD:
13.2 MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole eflluent toxicity tesL On average, a significant difference occurs for Environmental Testing Solutions, I chronic toxicity tests when a toxicant reduces Ceriodaphnia reproducton by 9.7% from the control Lower PMSD bound determined by USEPA (10a percentile) -I1%.
Upper PMSD bound detennined by USEPA (90a percentile) - 37%
The lower and upper bounds were calculated by the USEPA using 393 tests conducted fom 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-004003. US Environmental Protection Agency. Cincinnati, OH.
Environmental Testing Solutions, Inc.
Statistical Analyses Cerlediaphls Sursival asd Repbdactio Tert-Rcpreduedo Star Date:
Il/U12D05 TestD CdNC*R.
S"ple ID, REF-ReffToxcard End Date:
I IAV2005 Lab ID ET-Ena*. Testing Sol Samplb Type:
NACL-Sodinm chkide Sampl Dat:
Probocot FWCHR.BPA4321-R-02-013 Tesd Spcies:
CDCebdqapln dubk Came-eagIL I
2 S
4 S
a 7
a 9
1 D-Contrat 31.000 34.000 26.000 30.000 25.000 30.000 25.000 29.000 27.000 27.000 600 2t.000 29.000 33.000 27.000 29.000 30.000 26.000 29.000 23.000 33.000 tOO 29.000 30.000 27.000 27.000 29.000 29.000 27.000 26.000 2t.000 27.000 1000 32.0D0 31.000 26.000 29.000 29.000 23.000 23.000 23.000 27.000 24.000 1200 22.000 24.000 21.000 19.0O0 7.000 13.000 6.000 5.000 15.000 9.000 1400 7.000 8.000 14.000 12.000 7.000
.000 14.000 9.000 9.000 3.000 Trasforu: Untrans^fa ed Rant l-Tiled lIet kc Ce mmglL Meso N-Mesn Mesa Mli Mar CV%
N Sam Critical M es N-Mes D-ContlRO 28.400 1.0000 2B.400 25.0D0 34.000 10.259 10 28.B00 1.00DO 600 29.200 1.02t2 29.200 26.000 33.000 7.876 10 113.00 75.00 23.t00 2.0000 3OO 27.900 0.9924 27.900 26.000 30.000 4.612 10 102.00 75.00 27.900 0.96tt 1000 26.700 0.9401 26.700 23.000 32.000 12.860 10 90.00 75.00 26.700 0.9271
- 1200 13.100 0.4613 13.100 5.000 24.000 51.579 10 55.00 75.00 13.100 0.4549
- 1400 9.500 0.3345 9.500 7.000 14.000 29.461 10 55.00 75.00 9.500 0.3299 Aaxlflary Tests Seatisde Crideal Skew Kurt Koikzoprov D Test kihdirtes noeal dirbutan (p > 0.01) 0.742999S5 1.035 0.57349241 1.15B46555 Bardte Test indite unequal wances (p - 1.192-04) 25.3599415 15.0B62722 HlypstitaI Teat (l-tall, i.05)
NOEC LOEC CkV TI)
Sbeefr Many-One Rank Test I000 1200 1095.44512 Treatmnst va D-Conrol LUaewlaterpelstbes (200 Re smples)
Point ma/L SD 95% CL Skew IC05 890 110.963805 675.69375 1017.77749
-0.6514 I11 1011.47059 43.5475413 869.819444 1037.63214
-1.6247 ICis 1032.64706 20.514908 983.356136 1059.1445
-1.5647 1.0 IC20 1053.32353 17.4425533 1013.4339 1080.63192
- 0.1269 0.9 IC25 1075 18.7206497 1041.43686 1116.95268 0.2515 IC40 1133.52941 26.3589432 1100.74816 1215.26767 1.1061 0.
IC50 11B0.32235 36.6S6S776 1133.3B223 12B9.27212 1.1729 t1 0.1 0
50C
- t. O 100500 Oss 0Do lt DR p "0Pb 11-01-05
Environmental Testing Solutions, Inc.
Statistical Analyses Used torPMSD calatlon only.
Ceflodaplala Survival end Repnductlen Teat-Reproduetio.
Start Date:
I 1/1200 Teat ED:
Cd1~eClCR Sample ID:
REPPRcfTnecant Bnd Date:
I W2005 Lab ID:
BTSBEnvir. Testng SaL Sarple Twe:
NA 2L chladkum ide Sample Date:
Protocol:
FWCER.NPA-821.R.02-013 Tet Species:
CDndrioda*" dubia Cmmncnts:
Cone-mg/L 1
2 3
4 S
6 7
a 9
10 D-Cotrol 31.000 34.000 26.000 30.000 25.000 30.000 25.000 29.000 27.000 27.000 600 2B.000 29.000 33.000 27.000 29.000 30.000 26.000.
29.000 23.000 33.000 0OO 29.o0o 30.000 27.000 27.000 29.000 29.000 27.000 26.000 23.000 27.000 1000 32000 31.000 26000 29.000 29.00 23000 23.000 23.000 27.000 24.000 1200 22000 24.000 11.000 19.000 7.000 13.000 6.000 5.000 15.000 9.000 1400 7.000 t.000 14.000 1+/-000 7.000 7.000 14.000 9.000 9.000 8.000 Trenstorm Ustrensformed 1-Talled Core-mglL Mean N-Mean Mean Ml.
Mal CV%
N t.Stat Critleal MSD D-Cotraj 28.400 1.0000 2S.400 25.000 34.000 10.259 10 600 29.200 1.02B2 29.200 26.000 33.000 7.t76 10
-0.48S 2.2S7 3.751 S00 27.900 0.9S24 27.900 26.000 30.000 4.612 10 0.305 2.237 3.751 1000 26.700 0.9401 26.700 23.000 32000 12B60 10 1.036 2.2S7 3.751
- 1200 13.100 0.4613 13.100 5.000 24.000 51.579 10 9.32S 22B7 3.751
- 1400 9.500 0.3345 9.500 7.000 14.000 29.461 10 11.523 2.237 3.751 AnxillaryTests Statistic Critical Skew Kart Kolnogx D Tet indicates nI diaibuiix (p >0.01) 0.742999S5 1.035 0-5749241 1.15S46555 Berlett' Tet indicates unequal vances (p - 1.19E-04) 25.3599415 15.0B62722 Hypothesis Test (1-ll, 0.05)
NOEC LOEC CkV TU MSDm MSDp MSB MSE F-r'ob dr Dunnett Tedt 1000 1200 1095.44512 3.7506703 0.13206586 767.706667 13.4513519 25B-20 5,54 Treatments vs D-Comrol 11-01.05
Environmental Testing Solutions, Inc.
Page 5 of 6 Environmental Testing Solutions, Inc.
Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:
hi 2-Daily Chemistry:
I Day I
I I
I
-I
.1.
Analbst I Concentration IParameter pH (S.U.)
DO (mg/L)
CONTROL HASLO Conductivity 9/V (Almhos/cm)
Alkalinity 9
(mg CaCO3/L)
Hardness (mg CaCO3JL)
.I 2-Temperature (0C)
I I
I I
pH (S.U.)
I6L DO (mg/L) 600 mg NaCIUL Conductivity
.Temperatvre 7'.
i4.
J.6b Z
t1
.° (0c )
pRH(S.U.)
4 DO (mg/L) 800 mg NaCVL Conductivim (Punhos/cm)
Temperature z
- 2.
2.
(0C )
pH(S.U.)
_____5
-S 9
27 C
DO (mgL)
.2
.9 Zi5 1000 mg NaCl/L Conductivity (gmnhos/cm)
Temperature zq.-j Z!4.v LtlN al.e
-z
.2S Temperature
(°C) 2zLIV SS.O
- 28.
Z
-H SU.
i-
.7
+,~
DH (S.U.)
I.
I f.56 1-.6s DO (mg/L) l
-S 1400 mg NaCI/L Conductivity (utmhos/cm) 2130 ar -- ~~~-
~
L.-
Temperature (0C) z4.1 2S. O ZLI.S*
.T I
I
.I ii I
2{.1 2¶. 1 0-III Mfga I
STOCK Conductivity I (,mhos/cm) 62,1000 Initial I
Fi I al Initial a
Initial
Environmental Testing Solutions, Inc.
Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:_ A_2_
Day_l 3
4
_17__4_l^74 5
1 6
Analyst I_
14,9>
Concentration Parameter pHl (S.U)
+5
.o
.e 7
./
c DO(m L L.
'7 CONTROL Conductivity 3
St (atmhos/cm) t4~
& Z)Alkalinity (mg CaCO3 1.L)
Hardness (mg C CO3/Q 06L)
CS l
Temperature
.pH (S.U.)
1^Z
.o
.Y 79 75).g F5 1/
1
.DO (g/L)
IS 5
- 7. 5
- iCe
.S}
600 mg NaCIIL Conductivity (gmuhostcrn)
L-1 z
Temperature 2
Z
- Z.
253 25.1
_ _ _ _ _ _ _ _-_S C) pH (S.U.)
-,+1 /
7.4 I I./]
-IA6 DO (mg/L) 800 mg NaCU/L Conductivity 1 (pmhos/cm) IKEj 7.5 1a6i 2-Z-
-n Temperature (OC)
I -M.-L-q I
I I
I I
C, I -
I-
P IP - I pH (S.U.)
? 4 I
It I.7.51 I 7.GI - I
.o-I
'./O I -i.qq DO (mg/L) 1-. (Z.
1000 mg NaCVL Conductlvity i2/O (Gimhos/cm)
IZV q,?
757 z:
- z.
7-6' z1 ZZA.6 I1 ZZ" '10
?-S.Oa ZS I
tz Temperature (c)
A.I 2S.0 I
2ZS.0 I P
4-r..
DH (S.U.)
I1 DO (m;/L) 1200 mg NaCUL Conductivity (Amhos/cm)
_s_
ZcoJ.LO S.0 I
]
f l
-7.61 I
-zq.q 2*4.
Temperature (00 Y.oi/
15-a ' L i II -,M s'z-I 4-
_ 4 0
pH (S.U.).
1400 mg NaCI/L DO (mg/L)
Conductivity (gmhos/cm)
I-(,2-1;.1 21$6
?8 2
-S 2SZ-2.0 Temperature (0C)
ME r7.6 VI.
Z4-(
Final Initia I
3 1
1 53O l_
2.O Z28.1P Z.
&- 5.o--
aSn4 e
10.-
SO/Oe-.
I STOCK I Conductivity
_k1
-_I (umhos/cm)
I Izto1r Initial H
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: December 13. 2005
- 1. Facility / Discharger: Seguovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 3.266
- 7. 1Q10: 2,992.4
- 8. Outfall Tested: 101
- 9. Dates Sampled: November 14-19. 2005
- 10. Average Flow on Days Sampled (MGD): 1560.9, 1529.0, 1499.9
- 11. Pertinent Site Conditions:
Towerbrom 960 (oxidizing biocide) was injected from November 14-19, 2005. The dates and times for the Towerbrom 960 injections are in the following table.
Injection Location Date/Start Time (ET)
Date/Ending Time (ET)
Essential Raw Cooling Water (ERCW) 11/14/2005 0000 11/15/2005 2310 TrainAandB 11/16/2005 0920 11/19/2005 2400 Raw Cooling Water (RCW) 11/14/2005 0000 11/19/2005 2400 Note: Essential Raw Cooling Water (ERCW) Train A and B is located outside the plant and had to be shut down from 11/15/2005 at 2310 to 11/16/2005 at 0920 due to a NOAA issued tornado watch.
- 12. Test Dates: November 16-23. 2005
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimeghales promelas)
Daphnids (Ceriodavhnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 UV treated Intake: 100.0
- 16. Permit Limit Endpoin (%): Outfall 101: IC,5 = 45.2%
- 17. Test Results: Outfall 101: Pimephalespromelas: IC? > i 00%
Ceriodaphnia dubia: IC25 > 100%
UV treated Outfall 101: Pimephales promelas: ICQ, > 100%
- 18. Facility
Contact:
Stephanie Howard Phone #: (423) 843-6700
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected November 14-19, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
2
METHODS
SUMMARY
.Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/
(MM/DD/YY)/
Arrival Initial (MM/DD/YY)/
Sample Time (ET)
Time (ET)
Temp.
TRC*
Time (ET)
ID Collected Received Used By 101 11/14/05 0615to 11/15/05 1450 1.9,2.6'
<0.10 11/16/05 1358 11/15/05 0515 11/17/05 1302 Intake 11/15/05 0530 11/15/05 1450 2.0
<0.10 11/17/05 1302 101 11/16/05 0630 to 11/17/05 1415 1.5, 1.it <0.10 11/18/05 1318 11/17/05 0530 11/19/05 1300 Intake 11/17/05 0530 11/17/05 1415 0.8
<0.10 11/19/05 1300 11/18/05 0545 to 11/20/05 1312 101 11/19/05 0445 11/19/05 1355 1.6,1.8t
<0.10 11/21/05 1305 11/22/05 1310 11/1805 065 to11/20/05 1312 Intake 11/11/05 0515 11/19/05 1355 1.0
<0.10 11/21/05 1305 11/1/05 51511/22/05 1310
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.00C) in a warm water bath.
Aliguots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.
3
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1. Source:
- 2. Age:
Test Method Summary:
- 1. Test Conditions:
- 2. Test Duration:
Aquatic BioSystems. Inc.
23.0-24.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old Static. Renewal 7 days In-house Cultures
<24-hours old Static. Renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic 10 1
11/16/05 - 1237 ET
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10
- 6. Test Initiation: (Date/Time)
Outfall 101 11/16/05 - 1358 ET UV Treated Outfall 101 11/16/05 - 1344ET
- 7. Test Termination: (Date/Time)
Outfall 101 11/23/05 - 1306 ET UV Treated Outfall 101 11/23/05 - 1322 ET
- 8. Test Temperature: Outfall 101:
Mean = 24.9C 11/23/05 - 1151 ET Mean = 25.00C (24.4 - 25.20C)
(24.7 - 25.40C)
Test Temperature: UV-Treated Outfall 101:
Mean = 25.0C (24.3 - 25.40C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinneyville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic,/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 16-23. 2005 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - da
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 98 10.98%
100 100 100 100 100 98 98 22.0%
100 100 100 98 98 95 95 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100 100 100.0%
100 100 100 98 98 98 98 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg)
(% Effluent) 1(rplicate number)
( ffet)1 2
3 4
Mean Control 0.663 0.653 0.566 0.648 0.633 10.98%
0.617 0.663 0.677 0.689 0.662 22.0%
0.629 0.699 0.704 0.671 0.676 43.9%
0.689 0.636 0.701 0.704 0.683 72.0%
0.687 0.713 0.704 0.775 0.720 100.0%
0.618 0.705 0.661 0.699 0.671 Intake 0.689 0.607 0.642 0.583 0.630 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = I O/LC50: TUc = 100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 16-23. 2005 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions
(%Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100-100 I 100 100 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100 l100 I100.0%
100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(%Eflen)Data (rpicate nmer)
(%Effluent)
T 2
3 4
5 6
7 7
8 9
10 Mean
-Control 29 31 36 27 32 32 29 28 31 27 30.2 10.98%
31 35 32 30
- 30. 34 31 31 32 33 31.9 22.0%
34 32 32 32 33 31 33 32 34 30 32.3 43.9%
37 30 33 29 30 34 30 33 33 33 32.2 72.0%
35 31 29 32 38 34 35 33 29 36 33.2 100.0%
35 39 36 34 32 32 33 32 36 35 34.4 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 45.2%
Permit Limit: 2.2 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA ITUa = 100ILC50: TUc = 100/ IC25 6
TOXICITY TEST RESULTS '(see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 16-23. 2005 using water from Intake Percent Surviving Test (time interval used - days)
Solution (IEffluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 Intake 100 100 I 100 I loo I 100 I loo I 100 Test Solutions Reproduction (#young/female/7 days)
(% Efflu (replicate number)
_t) 1 2
3 4
- 5.
6 7
8 9
1 0 Mean Control 33 28 30 29 30 30 34 32 34 33 31.3 Intake 35 40 35 35 33 36 38 35 37 34 35.8 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LCso: TUc = 100/ IC25 7
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted November 16-23. 2005 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 98 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100 100 100.0%
100 100 100 100 100 100 100 Intake 100 I 100 100 100 100 TestSoluionsMean Dry Weight (mg)
Test Solutions rlicate number)
(% Effluent) 1 2
3 4
Mean Control 0.1562 0.718 0.688 0.670 0.660 10.98%
01732 0.656 0.551 0.547 0.622 22.0%
0 638 0.642 0.638 0.571 0.622 43.9%
0,525 0.602 0.776 0.563 0.617 72.0%
0.617 0.678 0.565 0.638 0.625 100.0%
01617 0.690 0.611 0.564 0.621 Intake 01602 0.618 0.621 0.580 0.605 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 1 00/LC5 0: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25)
Pimephalespromelas November 16-23, 2005 1416 7-days KCI 0.70 g/L Ceriodaphnia dubia November 1-8, 2005 1101 7-days NaCl 1.08 g1L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimnephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, November 16-23, 2005.
Test Sample ID Temper ture (C)
Dissolved 0xygen (mR/L) pH U.)
Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mglL CaCO3 ) (mg/L CaC03 ) Chlorine (mg/L)
Control 24.9 24.7 7.8 7.4 7.96 7.88 315 61 95 24.7 - 25.1 24.6 - 24.9 7.7 -
7.9 7.0 -
7.7 7.68 - 8.05 7.71 - 8.02 307 -
321 58 62 92 96 10.98%
24.9 24.7 7.8 7.4 8.02 7.83 296 24.8 - 25.1 24.4 - 24.9 7.4 -
8.0 6.9 -
7.5 7.92 - 8.10 7.62 - 8.00 288 -
303 20 24.9 24.8 7.7 7.3 8.02 7.83 282 24.8 - 25.1 24.6 - 24.9 7.4 7.9 6.7 -
7.6 7.93 - 8.10 7.66 - 7.96 276 -
288 43.9%
25.0 24.7 7.7 7.3 8.02 7.86 257 24.8 - 25.2 24.6 - 24.9 7.5 -
7.9 6.8 -
7.5 7.93 - 8.11 7.67 - 7.93 248 -
270 25.0 24.8 7.7 7.3 8.02 7.87 218 2.0 24.8 - 25.2 24.6 - 25.0 7.5 -
7.9 6.9 -
7.5 7.94 - 8.10 7.68 - 7.97 212 -
223 25.0 24.7 7.7 7.3
-8.02 7.86 181 63 72
< 0.10 100.0%
24.8 - 25.2 24.6
- 24.9 7.5 -
8.0 6.7 -
7.7 7.95 - 8.10 7.68 - 7.96 175 -
186 62 65 70 74
<0.10 -
<0.10 Intake 25.0 24.8 7.8 7.3 8.01 7.86 181 63 75
<0.10 24.9 - 25.2 24.6 -
24.9 7.6 -
7.9 6.9 -
7.7 7.94 - 8.09 7.68 - 7.97 173 -
187 61 64 72 76
<0.10 -
<0.10 Control 24.8 25.1 7.8 7.8 7.96 8.00 315 61 95 Control 24.7 - 24.9 24.9 - 25.3 7.7 -
7.9 7.5 -
8.0 7.68 - 8.05 7.93 - 8.07 307 -
321 58 62 92 96 10.98%
24.8 25.2 7.8 7.9 8.02 8.02 296 24.7 - 25.0 24.9 - 25.3 7.4 -
8.0 7.5 -
8.2 7.92 - 8.10 7.96 - 8.09 288 - 303 24.9 25.2 7.7 7.9 8.02 8.02 282
__22.0/.
24.7 - 25.0 24.9 - 25.4 7.4 -
7.9 7.6 -
8.3 7.93 - 8.10 7.99 - 8.11 276 -
288 24.9 25.1 7.7 7.9 8.02 8.03 257 43.9%
24.8 - 25.0 24.8 - 25.2 7.5 -
7.9 7.6 -
8.3 7.93 - 8.11 7.97 -
8.11 248 -
270 72.0%
24.9 25.1 7.7 7.9 8.02 8.06 218 24.7 - 25.1 24.9 - 25.3 7.5 -
7.9 7.5 -
8.1 7.94 - 8.10 7.99 -
8.14 212 -
223 100.0%
24.9 25.1 7.7 7.9 8.02 8.08 181 63 72
< 0.10 24.7 - 25.1 25.0 - 25.2 7.5 -
8.0 7.5
- 8.3 7.95 - 8.10 8.01 - 8.16 175 -
186 62 65 70 74
<0.10 -
<0.10 Intake 24.9 25.1 7.8 7.9 8.01 8.06 181 63 75
< 0.10 24.8 - 24.9 24.8 - 25.3 7.6 -
7.9 7.4 -
8.1 7.94 - 8.09 7.98 - 8.13 173 -
187 61 64 72 76
<0.10 -
<0.10 Overall temperature CC)
Pintephales promelas Ceriodaphnia dubia Average 24.9 25.0 Minimum 24.4 24.7 Maximum 25.2 25.4 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, November 16-23, 2005.
Test Sample ID Temperture (C)
Dissolved Oxygen (mg/L)
H S.U.)
Conductance Initial Final Initial Final Initial Final (pmhos/cm)
Control 24.9 24.8 7.8 7.4 8.01 7.83 305 24.9 - 25.1 24.6 - 24.9 7.6 -
7.9 7.0 -
7.6 7.92 - 8.11 7.66 - 7.98 290 -
314 10.98%
25.1 24.9 7.8 7.3 8.02 7.84 297 25.0 - 25.2 24.6 - 25.2 7.6 8.0 7.0 -
7.5 7.92 -
8.12 7.70 - 7.96 289 -
305 25.1 24.9 7.8 7.4 8.02 7.87 283 I
224.9
- 25.3 24.6 - 25.1 7.5 7.9 6.8 7.6 7.93 -
8.12 7.66 - 7.98 275 -
291 E
43.9° 25.1 24.8 7.8 7.3 8.02 7.85 258 a
24.9 - 25.2 24.5 - 24.9 7.6 7.9 6.8 7.6 7.93 -
8.12 7.65 - 7.98 247 -
274 72.0%
25.1 24.8 7.8 7.3 8.02 7.87 220 24.9 -
25.3 24.6 - 24.9 7.6 -
7.9 6.9 -
7.5 7.94 - 8.12 7.71 - 8.02 217 -
224 100.0%
25.1 24.8 7.8 7.3 8.02 7.87 185 100.0%
24.9 - 25.3 24.3 - 25.1 7.7 -
8.0 7.0 -
7.6 7.94 - 8.12 7.73 - 8.03 182 -
188 Intake 25.1 24.8 7.8 7.4 8.02 7.90 182 1 24.9 - 25.4 24.5 - 25.1 7.5 8.3 7.2 -
7.8 7.90 - 8.12 7.70 - 8.02 176 -
191 Overall temperature CC)
Pimephales promelas Average 25.0 Minimum 24.3 Maximum 25.4 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected November 14-19, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephales promelas and 2. Ceriodaphnia dubia Environmental Testing Solutions, Inc. performed chronic toxicity tests from November 16-23, 2005. The toxicity tests were conducted with samples collected from SQN Outfall 101 during oxidizing (November 14-19) biocide-applications. An internal peer review of the reports was performed by TVA biologists and the Senior Toxicologist and it was determined that incorrect serial dilutions were prepared. Previous permit dilutions of 10.98, 22.0, 43.9, 72.0, and 100%
were used instead of the dilutions of 11.3, 22.6, 45.2, 72.6, and 100% specified in the recently renewed permit, effective September 1, 2005.
The dilutions that were used in the tests were virtually the same as what the current permit requires (< 1.3% effluent difference) given the variability inherent in performing whole effluent toxicity tests. Additionally, the error is judged to be inconsequential relative to evaluation of the test results since no toxicity to either species (Pimephales promelas or Ceriodaphnia dubia) was observed (i.e., IC25 > 100% effluent) during either test period.
A process change to TVA's NPDES biomonitoring compliance program has been implemented whereby the contract laboratory must confirm via email to TVA the exact dates that toxicity tests will be performed and the dilution series that will be used, prior to conducting all future tests.
12
This incident has been assessed as a laboratory deviation. The EPA chronic test manual (EPA-821-R-02-013) states that deviations must be evaluated on a case-by-case basis and deviations may or may not invalidate a test result "depending on the degree of the departure and the objective of the test." TVA biologists and the Senior Toxicologist have considered the degree of the deviation in this particular case and find no potential or observed impact on the test results.
Therefore the test results for the subject tests are considered valid.
DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimeyhales promelas None
- 2. Ceriodaphnia dubia None PHYSICAL AND CHEMICAL METHODS
- 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by EPA Method 170.1.
- 5. The pH was measured by EPA Method 150.1.
- 6. Conductance was measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
13
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCI.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nuclear Plant Biomonitoring November 16-23, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 19, 2005 Sodium,-'
Cu CL41pFosta-1 Towerbrom PCL41Cl36 Cuprostat-H-130 Date mg/L mg/L..!
mg/L mi/Le mg.
.4 TRC ~
..~
Azole Qut 03/12/1998 2
0.016 s
03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 f :
03/18/1998 0.018 09/08/1998 0.015 0.014 >-
0.005 0.021 09/09/1998 0.003 0.031 t
0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998
< 0.001 -.
0 0.015 S
09/13/1998
< 0.001 -
0.044 -
0.015 09/14/1998 0.008 0.044-0.015 02/22/1999
<0.001 ;
02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 r 02/26/1999 0.008 02/27/1999
<0.001.
02/28/1999
< 0.001 08/18/1999 0.015 0.069 0.024 0.006.
08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070, 0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.006:
08/24/1999 0.016 0.067 0.023 0.020 2
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 19, 2005 Sodm Towerbrom PCL-PCLA401 C1pros3t-H-
Date fYPOCh rt' mg/I.g/
mgii gt F
gL 032
- 0.002C t
Copolymer
-DM u
Azole at, 01/31/2000 0.002 0.026,,i 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006,
02/03/2000 0.008 0.027 ¢;
0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 1
< 0.002 0.027 0.009 02/06/2000
<0.002 0.027
0.009 07/26/2000
< 0.0057 0.055.
0.019 07/27/2000 0.019 0.05S 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000
< 0.0088 0.055W 0.019 07/30/2000
< 0.0076 0.055 0.0019 019 07/31/2000
<0.0152 0.055 0.019 0.006 08/01/2000 1
< 0.0141 0.055' '
0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005,
12/12/2000 0.0092 0.025 0.020 0.005>
12/13/2000
< 0.0120 0.025 ' t@:.
0.020 12/14/2000
< 0.0087 0.025 0.020 12/15/2000 0.0120
'0.025 0.020 0.005 12/16/2000
<0.0036 0.O25k 0.020 12/17/2000
< 0.0036 0.025,fi`
0.020 08/26/2001
- >eK 0.017 0.06 3/4 0.021 0.006i
08/27/2001
<0.0096 0.06 0.021 0.005 -
0.021 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 '
0.020 0.005 0.021 08/30/2001
<0.0123 0.06 0.021 0.005.
08/31/2001
<0.005 0.059.C~
0.020 11/25/2001
<- 0.0044 11/26/2001
<0.0119 0.024 Y Q 0.02 0.005 11/27/2001 0
.0137 0.023 l 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006' 11/29/2001 0.0132 0.024, 0.02 0.007.
11/30/2001
<0.0043 0.024 0.02 12/09/2001
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128 0.024 0.02 0.008 '
12/13/200 1
<0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007' 3
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 19, 2005 Sodium Towerbrom 2
C[,2 PCL-401 C
H-130M Date m*gc/L m
Cm2g/PL-401,
Cuprost t K
TRC Phosphat Copolymer DMAD~,`
uf 01/02/2002
<0.0079 0.023 @-
0.02 0.006>
01/03/2002
<0.0042 0.023' 0.014 01/04/2002 0.0124 0.024,,.
0.014 0.009..
01/05/2002
< 0.0042 01/06/2002
<0.0042 01/07/2002
<0.0089 0.024 0.014 0.006 02/24/2002
< 0.004 02/25/2002
< 0.004 0.023 0.023 02/26/2002 0.0143 0.023 0.023 0.007 02/27/2002
< 0.0041 0.023 0.023 02/28/2002
< 0.0041 0.024 0.008 03/01/20021
< 0.0041 0.024 ;
0.008 05/05/2002 05/06/2002 0.05 0.02 0.014 05/07/2002 0.058 0.0 15 -
05/08/2002 0.056, 0.019 05/09/2002 0.057 0.02 0.014 05/10/2002 1________
0.056 -
0.019 08/04/2002
<0.0058 08/05/2002
<0.0058 0.053 0.018
.0.025 08/06/2002 0.0092 0.053 0.018 08/07/2002
<0.0107 0.055 0.019 0 007 08/08/2002
<0.0061 0.055 0.019 08/09/2002 0.0152 0.054 -
0.018 0008 ^
10/06/2002
<0.00497 I
10/07/2002 6
0.0153 l
0.054 0.018 0.009 l
10/08/2002
<0.0092 0-054 0.018 0-007 10/09/2002 0.0124 0.053 0.018
..0.009>
10/10/2002 0.0134 0.054 1 0.018 0.009 10/11/20021
<0.0042 0.054 0.018 01/12/2003
<0.0035 I
01/13/2003
<0.006 0.025 :
0.019 0.009 l 01/14/2003
<0.0118 0.026 0.020 01/15/2003
<0.0063 0.026 0.020 0.009 01/16/2003
<0.0034 0.026 0.020 01/17/2003
<0.0034 0.026 0.009 04/06/2003
<0.0073 T
04/07/2003
<0.0189 0.021 04/08/2003
<0.0117 0.021 04/09/2003
<0.0139 0.021 0.016 04/10/2003
<0.0113 0.021 0.018 04/11/2003
<0.0073 0.022 4
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 19, 2005
-Sodium-,"'
11
-M Towerbrom PL22.
PCL-401 CL-3 Date Ilypochlonitel....I Daemg/L mgLw xg/L mg IW RC hopa Copolymer DMW 06/15/2003 06/16/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003
< 0.0045
< 0.0037
< 0.0048
< 0.0048
< 0.0085
< 0.0048 0Os7; 0.041 0.041 0.058.:'
0.058 0.020 0.014 0.014 0.020 0.020 08/03/2003
<0.0050 08/04/2003
<0.0050 0.058, 0.020 08/05/2003
<0.0051 0.057
,,' 0.020 08/06/2003
<0.0084 0.057-0.020 08/07/2003 0.0129 0.057 0.020 08/08/2003 0.0153 0.057'+
0.020 0.009' 10/05/2003
<0.0043 0.057 0.020 10/06/2003
<0.0043 0.057 0.020 10/07/2003
<0.0090 0.057 0.020 10/08/2003
<0.0106 0.057 0.020 10/09/2003 -
0.0181 0.026 0.022 10/10/2003 0.0183 0.026 0.024 0.009 :
02/01/2004 0.0093 0.027 0.009 02/02/2004
<0.0034
--1 0.026 0.009 02/03/2004
<0.0034 O0.026>.
0.009 02/04/2004 0.0124 0.026 0.009 0.009 02/05/2004
<0.0034 0.026'",
0.009 02/06/2004 0.0105 0.026 0.009 0.010 -
05/04/2004
<0.0123 0.026 S'-
0.019 05/05/2004,
<0.0144 0.026 0.014 0.009 05/06/2004
<0.0146 0.037.
0.013 05/07/2004 0.0227 0.058:,
0.020 0.009 05/08/2004 0.016 0.060 0.021 j
05/09/2004
<0.0104 0.058 0.020 07/04/2004 0.0217 0.057-"-
0.019 07/05/2004
<0.0085 0.057 0.020 0.009 07/06/2004
<0.0077 0.058, 0.020 07/07/2004 0.0252 0.056 0.019 07/08/2004 0.0223 0.057 0.019 0.009 07/09/2004 0.0182 0.057 0.020 0.009 5
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks, During Toxicity Test Sampling March 12, 1998-November 19, 2005 Sodium.,-Nac Hypoclilit Towerbrom
'PC PCL-401
- 6 Cuprostat-PF H-3M 73551 Date
- e.
mg/L mg/L mg/L
,gL mg/L mg/L mgL.
- m mg TRC
- Phosphat, Copolymer DMAD Azole QEO/PO Quatf T R C '
s 11/07/2004
-,-<0.0187 0.000 a
0.014 11/08/2004
<0.0192 0.047, 0.030 11/09/2004
<0.0233 0.048, 0.016 0.04i-11/10/2004
<0.0149 0.047, 0.016 0.041 11/11/2004
<0.0149 0.049 0.017 0.043 11/12/2004
<0.0253 0.048'.
0.017 0.042 02/06/2005 0
0.0042 0.028 0.010 S
02/07/2005
<0.01 16 0.028 0.010 0.007 02/08/2005
<0.0080 0.028:
0.010 02/09/2005 0.0199 0.028 0.010 02/10/2005
<0.0042 0.028'-
0.010
- i.
02/11/2005 0.0155 0.028' 0.010 0.007 06/05/2005 0.0063 A
06/06/2005 0.0043 i
- 0. 037 06/07/2005
@ 0.0103 0037 06/08/2005 0.0295 0.037 06/09/2005 0.0129 06/10/2005 0.0184 07/17/2005 0.0109 0.026 -..
0.009
-9 07/18/2005 0.0150 0.026,-
0.009 0036 07/19/2005 01 006 0.009 0.036 07/20/2005 0.0209 0.026,--;
0.009 0 0.014 0.036 07/21/2005 0.0242 0.026 0.009 07/22/2005 0.0238 0.054 0.018 0.014 10/30/2005 0.0068 10/31/2005 g
0.0112 11/01/2005 0.0104 7
0.035 11/02/2005
-. r.
0-0104 0.036.
11/03/2005 0.0117 0.036 Y 11/04/2005 0.0165 0.035 11/14/2005 0
0.0274 11/15/2005 0.0256 11/16/2005 0.0234 11/17/2005 0.0231 11/18/2005 0.0200 11/19/2005 0.0116 6
Sequoyah Nuclear Plant Biomonitoring November 16-23, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
hIOMONITORING PRIN OFoCfUSTODY ECOD rage_1_ofI Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number. N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 NPDES Number TNO026450 Phone:
828-350-9364 Collected By: Duane Brigman; Chevy Ward Fax:
828-350-9368 Field Idendfication /
Grab/Comp.
Collection Dateirune Conbtai Flow Sample Description Nun*_&e MOD Rain Event?
'r.J w.ose Volmne(Mark as Appropriate)
DoeTime Yes if Yes.
No Traee T
o A&WeTaew.
Or ibis Aper SQN-101-TOX Comp 11/14/05-11/15/05 0615-2 (2.5pl)
NA X
=-
t
_0515 lot;___
SQN-INT-TOX Comp 11/14/05-11/15/05 0630-1(2.5 gal)
NA
- X.o C 0530 A"c Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Tine Received By ignature):
eime 11/15/05 7.i 5
2St 11115105Express Courier Express Courier 11/15/05 ETS 1111105 A15Z
/
J, mif t
p Ltnee hi s eform eY., II t.k44d. &-w Aio.-
t~
~
col Dt5sp*
Instructions: Clients should fill in all areas except those in the "Laboratory Use block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time fb each sample is 36 hows from the time or collection. Therefore, please collect and ship in such a way that the laboratory will receive the sunples with ample time to initiate testing within that tine frhme. Samples shipped ovemight on Friday via FedEx or UPS must be narked for Saturday delivery or they will not arive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solution, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:
NPDES Number: TN0026450 Phone:
828-350-9364 Collected By: Duane Brigman and Chevy Williams Fax:
828-350-9368 Field Identification /
Grab/Comp.
Collecton Date/Time C
Flow i
Sample Description Number &
MGD Rain Event?
Lboratory Use Volume (Mark as Appropriate)
Date TMYes If Yes, No Trace ETS Log Anrival Temp.
By Time Appear-
_.__ll___
Colce inches lrnwhe XT-anc SQN-101-TOX Comp 11/16/05-11/17/05
&,:35a 2 (2.5gal)
NA 4*
ati ti.( (4 l
$7 SQN-INT-TOX Comp 11/16/05-11/17/05
&s30a.
1(2.5 gal)
NA R
Sample Custody - Fill In From Top Down l
C H
S Relinquished By (Signature):
Date/Time Received By (Signature):
Da rime Duane Brigman 11/17/05 Expr iCourie 11/17/05 hl
/0 GIL Express Courier
- 11/17/05 ETS
.11/17/05 IA1 4
4
, I Instructions: Clients should fill in all areas except those in the 'Laboratory Use" block. Biomonitoring samples are preserved by storing them at 61C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time fame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1_ of 1_
Client: TVA estg Solutio n, Inc.
Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street.
FedEx UPS Bus Client P.O. Number. N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:
NPDES Number TN0026450 Phone:
828-350-9364 Collected By: Duane Brigman and Chevy Williams FaX:
828-350-9368 Field ldentiflcation I Grab/Comp Collection Dateime Container Flow 0
2 Sample Description Number&
(MUD)
Rain Event?
U7T:
Volume (Mark as Appropriate)
.Y Use DateTim Yes if Yes, No Trace EMh Lok Arrival Tenp.
-,By.
ie Appear-inches SQN-101-TOX Comp 11/18/05-11/19/05 O45'5 2 (2.5gal)
NA SQN-INT-TOX Comp 11/18/05-11/19/05 0P4-t 1 (2.5 gal)
NA (3S 4
wl: e
..4..
Sample Custody - Fill In From Top Down i C o&
4 Relinquished By (Signature):
Date/Time Received By (Signature):
Dagime Duane Brigman 11/19/2005 Exp j s qurier,,
11/19/2005 Express Courier 11 /1 I29/2005 3, ETS (Vt 1
V 11/19/2005 (g55 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
.I.....=
Environmental Testing Solutions. Inc.
PagelI of 6 Environmental Testinf Solutions. Inc.
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #:
zz7c)
County: Hamilton Treatment: Non-treated Outfall: 101 Dilution prepara don information:
Comments:
Dilution prep (%)
10.98 22 43.9 72 100 Effluent volume 274.5 550 1097.5 1800 2500 (mL)
Diluent volume (ml.)
2225.5 1950 1402.5 700 0
Total volume (ml.)
2500 2500 2500 2500 2500 Test organism Information:
Test information:
Organism age:
r b mo A..S A oub Randomiiing template:
1 L 1 Date and times organisms 1%-ks "Os
-TOb SCO Incubator number.
were bom between:
C.
Organism source:
A6i BTroI I-I t5s.
Artemia lot number B6(71&
Transfer bowl information:
pH -
Temperature = ° C
Total drying time:
A. $AwS S._ o z4.
Date / Time in: fi -2 l05 I 5SO Average transfer volume:
ID. (
Date/Timeout l-2A-oS i3BS6 Oven temperature:
fdO C_
Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time MlASUD VSlo I3O il-It-osA
°SmlIS
.OL4OS I
-o
.. fl5 I50(e 150L It-ll-,6SA Os a I ks.O' 4 g 0 2
,I-8-cs, Q5 vszo 13 18 Os-O5-o; OSI rI. O' 6'-1
-I 3
J.-1Z0 084.
NQzil am 5I-ri-as At OsiIII-1O Ii-.4 O
A 4
11I-78-C 0qOS 13 1z Zlnt S111q.It4 13 11 21-65 090 I-SI 1305SO I 1-n.Q f, 10 Ilq.1Lt 4 1.3 6
'SOO l3lc)
I it-trlGS5&
aSI iL.tZ 4 13 7 _1 R-2.5-05 U
^.
_v Control information:
Acceptance criteria Summary of test endpoints:
I
% MortalityI o1tt S 20%
7-day LCso _
> I oj l Average weight per initial larvae:
O.f.3 e
NOEC _
Average weight per surviving larvae:
- 0.
13 42 0.25 mg/larvae LOEC
- 1CO7C, ChV IC25 1
) 160010
Environmental Testing Solutions. Inc.
Page 2 of 6 Environmental Testing SolatinnL Tn
Page 2 of 6 Species: Pimephalespromelas Date:
11-IL,-uc Client: TVA I Sequoyah Nuclear Plant - Non-treated Suruival and Growth Data Day CONTROL 10.98%
22%
A B
C D
E F
G H
I J
K L
1-
-D
-°
-° 0 10 lo10 lo lo lo l
o l t/J /0 10 II(D t/0 I(J I(
J IC) 10 10 I 0 1 *0 JO 25
/01 l 10 10 1010
/0 10/10 1
/0 to
/___
/0 to
/C6 to 41 0
4 tototo IL1I
(
lo o 0 1o0 110(to10 5
/i
/
I/O'
£0 I 0 to10
/0 el
'0 to10 6
/D IC0
/
/0 1
10 '0 ivq~/0 ID 1o to
-1Jo A - Pan weighitn(m) ll. 60 I Y. S21q)
Y. SC IY2l1q,13
'14z.S8 Color Z
Analyst:
JIJ N
A-ill 1.7o
- I.03 IS.o 3tJ B - Pan I Larvae weight Analyst:
11 21.23 Z92 20 1 2
Zo.93 21.33 Zlo.l Z1.43 2b.4Z at.Z Z 1.LMZ 21.-
Larvae weight (mg) - A - B 6.64I.
1653 S.X; 1t.42 1631 6.1 (.3t-.II 1p. seV(% 25 to.,q, 7.04 6.1l I
I I
Weight per Initial number of larvae (mg)'
- C / Initial number of larvae 0o 4
V 0'
%O IV 0I CO oN NO 4,
O?
AN O-V b¶1 OS Al-DI
- 9O q
V 0*
A\\
Oit I
Average Percent weight per reduction Initial from control 0.I number of
(%)
larvae (mg)
O. bb6 fZ
- '4.
0 VO
.1
}
I Comment codes: c - clear, d - dead, fg - fungus, k - killed, m - missing, sk - sick, sm - unusually small, Ig - unusually large, d&r = decanted and returned, w - wounded.
Calculations and data reviewed. lsi_
I Comments:
Environmental Testin2 Solutions. Inc.
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
Ilt-1(-TS Survaval and Growtk Data Day 43.9%
72%
100%
M lN l
O2 R
S T
U I V I W x
0
/ O IO 0 o 1 0 / 0 I cj 1 0 lo 1 0 I 0o I O 1
JoC) 10 I '
IC)°O I
0 IC) 1 0
10O 2
1
/
/
/O
/
/°
/O lo 1 10 t o 3
/
/ -
6
/6
/O
/0/
,o /0)0
/O 40
)
lo
,6 lo
/
/ 10 10 1/o 1/
5 10 t 10
)1 /
1 10010 ID 00 6
10 It 10 tO I 0 /O
/O to I0
/0 q
/O 7
& -IjS -
.10 10 Jo 0
1' 1
IL) 10 1c
/o q_ Jd_
A-Pan weght (mg)
- 1.
,81.o II'.
7 15.05..
Color identiflcationOM(AL
- 11. 54 Analyst:
JP 5rSIyt4 15.6G_1;13 56 B-Pan + Larve weight (mg)
Z151 22P0 2i.2 Analyst:.11,4V~lt W ~ J3 fl-I5 21(ol ZI.SI 2.1i" LZ.Zq £11 2-~ZIZ t9 ZI.i 22kB6 Larvae weight (mg) - A - B 6-02 (e..6b I-Di 1.0i tb.a
-i-w1.1 1 A 1.S I0.- le 1.t0 S 16.61 Ie.M Weight per Initial number of larvae (mg) tr' 7
-C / Inidal number of lirvae 0
O O-O' Average Percent weight per Ireduction initial from control 0.i
-I.'
37a 0.120
-13.87O 0.o v1 t
_ OX6%
number of
(-
larvae (mg)
Comment codes: c = clear, d = dead, fg - fingus, k = killed, m = missing, Ak = sick, sm - unusually small, Ig - unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Page 4 o~f 6 Environmental Testina Sohitions. Inc.-
Enviranmental Tetfno nhtinnq Tn
Page 4 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date:
II\\- I 6-6; Survival and Growth Data Day 100% Intake Y
Z AA BB 0
10 1IC)/C)lt
°10 I
1 10
/O
/0
/0 10
/O I1 10 IC() /O ID O
6 1to
/0 1 0 10 7
d L
A - Pan weight (mg)
I q 15,23 Color Identificaaiion:
Analyst:
Jt I3S iq.7 B - Pan + Larvae weight (mg) 1S 2 l2 b20Z Larvae weight (mg) - A - B Weight per Initial number of larvae (mg) es C I Initial number of larvae
'a Average Percent weight per reduction Initial from control
- 0. e0 number of
(%X
)
larvac ng Comment codes: c = clear, d - dead, fg - fungus, k = killed, in - missing, sk sick, sm - unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.
Calculations and data rcviewed:<C&
I I
I Comments:
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number:
2220 Revetwed by:
Not for Compliance Assessment, Inte nal Labor. toL y QC.
Coiee.1rall"ah Replicate Iilia samberof Floaatambe of APanwWeght B-iaf+Lavme Larvaewielhl(ox Weighi/S.rblas Mean weigh I Coeffideat(varaida WeighsIllttalo-ber kMea.,avlval Mra aetin CIaefikealaI Peeeredaetin li0 lame larvae (mg) welge (0g)
-A-B samberdlarme(mg)
S0rviviilga..beref eW dlame(.g)
(%)
loIdalmemberaf varlalt.. pb_.w*egae eatl (%)
larvae t(as
)
larvae (mg)
P.rWs..e k.so A
10 10 14.60 21.23 6.63 0.663 0.663 Cotrl I0 1 0 15.09 21.62 6.53 0.653 0.4 10.65397506371Ntapibe Centrl CI 10 9
14.52 20.18 5.66 0.629 0.64 2.2 066975 0.633 7.1 Net applk6abe iD 0 10 14.75 21.23 6.48 0.648 0.648 E
10 9
14.56 20.73 6.17 0.686 0.617 10.9t%
F i0 10 14.70 21.33 6.63 0.663 Oh79 1.7 0.677 975 0.662 4fi
-4.6 109%
G I10 1 0 14.24 21.01 6.77 0.677 0690.6637.7.62d 4
H 10 10 14.54 21.43 6.89 0.689 0.689 I
10 9
14.13 20.42 6.29 0.699 0.629 22J I10 9
15.03 22.02 6.99 0.777 073630.699s00.7
.1-.
22%
K I 0 10 14.58 21.62 7.04 0.704 0.713 6.3 0.704 95.0 e.676 5.1
-6.t L
10 10 15.06 21.77 6.71 0.671 0.671 M
10 10 16.24 23.13 6.89 0.689 0.689 43.9%
N i0 1 0 15.79 22.15 6.36 0.636 0.683 4.6 0.636 100.0 0.63 4.6
-7.9 0
10 10 14.60 21.61 7.01 0.701 0.701
.P r
o 10 14.47 21.51 7.04 0.704 0.704 Q
10 10 14.88 21.75 6.87 0.687 0.687 7%R 10 10 15.16 22129 7.13 007205330.713 0.07253 1.
72%
S i
O i
O 14.47 21.51 7.04 0.704 0.72 0.704 I
0.0 0.72 5.3
-13t 7'
10 10 14.32 22.07 7.75 0.775 0.775 U
10 10 i5.05 21.23 6.18 0.618 0.618 100%
V 0
10 14.54 21.59 7.05 0.705 0.689 7.2 0.705 97S5 0.671 6.0
.6.0 W
I10 9
14.49 21.10 6.61 0.734 069720.661 X
10 10 15.69 22.68 6.99 0.699 0.699 Y
10 10 14.84 21.73 6.89 0.689 0.689 100% Itake 10 9
14.35 20.42 6.07 0.674 0.647 7.3 0.607942.6073O I
% Intake 10 to 15.23 21.65 6.42 0.642 00607
- 97.
0.a3o 7.3 0.4 BB_
10 10 14.79 20.62 5.83 0.583 0.53 Outfall 101:
Dunnett's MSD value:
PMSD i
Intake:
Dunnet's MSD valua:
PMSD:
MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.
On average, a significant difference occurs for Environmental Teating Solutions, Inc. chronic toxicity teats when a toxicant reduces Pimephales growth by 14.0% from the control (determined through reference toxicant testing).
Lower PMSD bound determined by USEPA (10th percentile) - 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) - 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pmephales growth in chronic reference toxicant tests.
0.0624 9.9 USEPA. 2000. Understanding and Accounting For Method Variability in Whole Ellbent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Statistical Analyses Larval Fisk Grow and Sorvia Twi Day Growth ADtte 11/16/2005 TintlD rpFRIFr2 Sumpkak 7VA/ SQ 104 NOmated Bad Data.
1I123R05 Lab lD.
17&Enh*.TuftrSoL Sanlpk1,p1:
D hl R4. M alkdagbpart Sampk Dat:
Prmtoot YWCER-BPA-121-R-02.013 Tat Spedes PP-Ptuphalu promelas C
.e.%
1 2
2 4
D4olrwl 0.6630 0.6530 M5660 0.6410 10.91 0.6170 0.6630 Q6770 0.6W9 22 0.6220 O.6990 0.7040 0.6710 433 C690 0.6360 0.7010 0.7040 72 0.670 l7130 0.7040 0.7750 100 0.6110 0.7050 0.6610 0.699O Tranoforu Uatrsaformed I-Taoed botonsk Comc.%
mum N.Mmx Me" 1mi Max CV%
N Isalt CA" 4
MSD Mm" N-Mes D-Cbotrol 06325 1.OOQO 0.6325 0.560 0.6630 7.071 4
0.6744 1.0000
.91 C06615
.Ot51 (Q6615 0.6170 0.6990 4.764 4
-1.105 2.410
.0.0633 Q6744 1.000 22 0.6751 1.0614 0.6753 06290 0.7040 5.041 4
-1.647 2.410 0.0633 06744 1OXO 43.9 0.6125 1.0791 6t25 06360 0.7040 4.640 4
.1.904 2.410 Q0633 Q6744 I.OXO 72 0.7191 1.1379 0.719t 0.6M70 0.7750 5332 4
-3.3323 2.410 Q0633 0.6744
.1OO 1to 0.6706 1.0605 06706 0.6180 0.7050 5.994 4
-1.457 2.410 0.0633 6703 Q9946 Alla7TsStatfsdc Chidea Skow IKArt Ihapim -WIkst di sam dktrb~lcm (F >.01)
Q92524302 0.84 4.56720 55 4.70t1241 Buartleta Test kidicatea sqwlvaunces (D -0Q99) 0.532311773 15.05627224 Ilypotkes Test (14UI.SAS NOEC LOEC OV TU MSDo MSDp MSB MSE FVhal di DimneW Test Dt100
>100 I
0.06373977 0100037909 0.003245742 0.001379623 0012390115 5,11 T1atmeals is D13onleml Lkeartepoaion PG REmmples) rolat SD ff% CLEp)
Skew 1005
>100 IC10
>100 1C15
>100 1.0 IC20
>100 0.9 IC25
>100 0.8 1040
>100 IC50
>100 0.7 0.6 0.2 Q2 0.I 0.0 I*6
- 01.
40.2 0
20 40 so so 100 120 Do" %
Deos.Rupoee Fbt u.a 0.6 0.7 0I Q5 0.4 0.3 0.2 0.1
-P
- 11.
146.1.0.06 ~
.. I af aI~dlaifloen o-I 4
1 9
- qej1-16-Osdota
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 16-23, 2005 Statistical Analyses L~rasyl Ik Crm&l and Sar*lvaJTe14U DaGrow~at Stat Date 11/16i200S Tat 1D.
PFR Sampk 1D:
IVA/ SQi ns Non-ted REd Date:
I l23f2005 Lab ID:
B vir. TtigS Smple 1TpM DI)MR.-Diacherg eckorkglpcft Semple Dale:
hrloo:
pWCHRMAPA-12-R-02-013 Tea Specis:
PF-PimePlA reomen Comments:
Crae%.
1 2
3 4
D-Contol 0.6630 0.6530 03660 0.6480 100 0.6190 0.6070 0.6420 03130 Tr:awr.L Uathufarw.6 I-Taledl lbetok Men W-M Men M
Ma CV%
N
=tdtt OCli MSD Me an NMen D-Conrol 0.6325 1.0000 0.6325 O5660 0.6630 7.07t 4
0.6325 1.0000 100 0.6303 0.9964 0.6303 03130 0.6190 7307 4
0.070 1.943 00624 0.6303 0.9954 A_ illaryTestg Sttgde Oldeal SLeW Kart Shapro-Wka Tea Jdkete
-1 ditabution
>0.01) 0946636319 0.749 44779630
- _7466024 P-athdleat:=
-=avelnca(P-0.96) 1.05616569 47.4672279 eti T t l-a4l S)
MSD MSDp MSB MgSE
-Freb Ur Hoaseedastic t Test indicates no agCntdieraee 0062403399 00M9161S 1.012SE05 0.002062625 0946420491 1,6 Treatments to D-Control Lmear ltesrpdoladeo (200 Rassmples) poine SD 05%aCxxp)
Skew IC05
>100 ICIO
>100 IC15
>100 1.0 IC20
>XW IC25
>100 0.9 IC40
>100 0.S ICIO
>10D 0.7 0.6 0.3 0.2 0.1 0.0 0
20 40 s0 D0 t00 120 Do" %
DosetRpoertslt 0., -
0.7 0.6 J0.5 C A
&0.4 is 0.3 02 0.1 14-a, 0.5O kW of *1grillowme 0O-8 srnj1I-6-05data
Jnvironmental Testing Solutions, Inc.
Page I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #:
Zvz-O County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparaion lnformation:
Comments:
Dilution prep (%)
10.98 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test Information:
Organism age:
7
£ O.1-t_ 0L b Randomizing template:
6__e____
Date and times organisms I-igO O
So I-i2-Incubator number and ZCI r
were born between:
shelf location:
_______Z Organism source:
I l-oBS A-FYCTbatch:
05 3 O5 Transfer bowl information:
pH = 1.1 Z. Temperature= z.,Ic. Selenastrum batch:
I-11 OS Daily renewal Information:
Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0
1-ail-OS i
I1-i-OsA os I l[S. O 9 OS o
I t8-n-o Il-1 I-l-OSS Osills.044
- S a
2
-t2-eii 1200 1i-11-os8 os I In-. oS4 04
.3 I-^o 1ls-z 11-l-OTA 0O111-1I.#OL 2
4 l C-)
Z2 16 El.1t-c e e~siij. f2.4 13 5
1 Zz It.r-s If~5 A 011197 17l 4 13
-#t 6
5 z4 12.Z1 II-1-4-05 05fli1q.l M413 6
I -z2-OS 1_.12 I)-n-656 cS
_q.
a 4i3 Control information:
1 2
Acceptance criteria Summary oftest endpoints:
% of Male Adults:
07d
- 07.
S20x.
7-day LCSO
> & ?t1 a
% Adults having 3' Broods:
ot7 wt a2o 80%
NOEC
- IooY,
% Mortality:
O%
0)5:
S 20%
LOEC Mean Offspring/Female:
XZ 31.5 k I s.o Offspring/female ChV
> I O'17,
% CV:
'.-1.
7.I
<40.0%
IC25
> I O07a O'xkre
Environment%] Testinlg Solutions, Inc.
Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date:
ii-lbc.
CONTROL -1 Survival and Reproduction Data Replicate number Day 1
2 3 1 14 5 6
7 8
9 10 I
Young produced Q
OQ J
J
_-O Adult mortality
(
U t
2Young produced
(:
0 O
O O
O Adult mortality LUL L
L I
L-Ll 3
lYoung produced l 0
O l
0
(-l(
(
)lO Adult mortality L
L L L 4
Young produced [o U
i@
5
_.,4 0
Adult mortality 1
LI I
L-I L
S Youngprodueed IClo I )
I 1II Adult mortality L
=
I1-
=
=
6 Young produced 0
l O
C)I lI z l o I o tO Adult mortality L-_
L-l 1
L UL-L 1-7 Young produced I
l(
r.o i
1t t Z-i i(o 3
Totalyoungproduced l'I Z
' i TziT 3Z. l32.
Z-I Z ZT Final Adult Mortality
\\..
~
7I_
X for3' Broods l
5-?
3, l
Note: Adult mortality (L - live, D dead)
-sp,-T e4ob Concentration:
I
-% Mortality:
l C)1.
Mean Offsprin emale:
l30..-_
CONC: 10.98%
Survival and Reproduction Data Replicate number Day I____ 1.
2 3
4 15 6
7 8
9 10 IYoung produced CL 0
C 0
-1 gprde Adult mortality L
1 L.!-
L...
2 Young produced l
lC) l(
l C
l l
l l
Z O
CJ )
0 0
JO O
O 0
Adult mort lity l
L Q
l l
L L-1*1+/-
3 Youngproduced l IO L)
I 0 2 I-0 l1l l
lAdult mortalityf L
l
_1L 8j-r lX§;
L l 4
Young produced 5
15 lI S L
l
.lAdult mortaiity lL-L-_
L-L I Youngproduced I 0 0
l 10 10 l0 12
_LlA Adult mortality L. -=
L-k-
l-L--
6 Young produced j I -
C1 r
a Adult mortality lT?
1J
_1
,-?
W
=-
y 6 Young produced I
ii...
0 O6 l LAi 01I0L YTotalyoung produced I
=3 5 Z 35 l 3 2 z
Final Adult Mortaliqty
?
W l
Note: Adult mortality (L - live, D - dead)
Concentration:
% oMrtality:
l 7,
-Mean Offspring/Female:
31.'q
% Reduction from Control:
. 67
Environmental Testing Solutions, Inc.
Page 3 of 7 Envionmnta TetingSoltios.
nc.Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date:
'l-S CONC: 22%
Survival and Reproduction Data j
~Replicate number Day 1
2 3
4 5
1 6 7
l S
9 to I
Young produced n
t D.
CI CI O
_ 0 Adult mortality U
U.
t
.>1.
k..
2 Young produced CD O
O co O
Adult mortality L
L L
L-
_L L-I1_
L 3
Young produced ol 0
0 To l
1Jl° Q7o o
Adult mortality L
L l t l I-4 Young produced L
s 5
1 5
L4
=4 5 I Adult mortality L
L L-.
L L
L-i L-1_
S Young produced l lO I Z-I A l O
O o
Adult mortailty l
l l
I 6
Young produced 0 C l
l O
I I I0l l
11 l
l O
l IC) 10 Adult mortality l
l
-_I l
U l
Ll
=
=
7.-
7 Youngproduced jc, 15 11 J
1 15 1S 1ii i'
IS Totalyoungproduced l44 3Zl 3Z l
33 3I
.5 33 3z lL T Final Adult Mortality I_
t_
-k-l C
l L
l l.-
Note: Adult mortality (L-live,D-dead) h1L wjUr.btceoI
- Concentration:
%Mortialitl o7.
l Mean Offspring/Female:
I 32.5 I
% Reduction from Control:
--1. 071 CONC: 43.9%
Survival and Reproduction Data Replicate number I
Day l
1 2
3 4
7 6
l 7
8 9
10 lI Yung produced O
C)
O 0
_I l
Adult mortality l _
L l
-t:
l
(
2 Young produced l
0 0
n 0 O C) 0 O l
=
Adultmort lty i
L_
L-lL-L-
3 Young produced (7) 0 e
l li I Adult mortality IL L
I I.-
4 Young produced]
LL..
L4 T4 'S j
14 S
S I
Adultmortality 1 L L
L L-L -
L-I L-L L -
IL S-
-Young produced j0~JC 0
It~
0 1 0
- 0) Ij r Adult mortality]
ULUL_
L-UL-UI L-L-
L-I
-7 77
- a.
=
7 6
Young produced 131 /
I Z l I c lC)C I 2 0
° 11 0
1 1
Adult mortality U1 UL-L-
U
-UU U-U-
=
=
=
=
--- Young produced i
iIs iS f7 0
IR V1 iG J 7 l1e Totalyoungproduced l 3-i b 7 3 3 l 33 Tl Final Adult Mortality Note: Adult mortality (L - live, D - dead)
Concentration:
% Mortality:
o7 Mean Offspring/Female:
3Z.M
% Reduction from Control:.
-(.i7,
Environmental Testing Solutions, Inc.
Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date:
ii. i b-c CONC: 72%
Survival and Reproduetion Data Replicate number Day 1
2 3
4 5
6 l
8 9
10 I
Young produced i 0
)
Of OLC O
O t
Adult mortality L
L L
I L-L L-2 Young produced r
)1
)()
lt) 2 e
lO Adult mortality L
L_
L 3
Young produced O
O o
J O
OŽ 0I Adult mortality L
L L,
L L
L-L 4
Young produced Q j T
t5 I (O LI 5
5o Adult mortality L
L-L i
L I
L S
Young produced I
£ l I o l4 1
.o I Adult mortality I
l F
l_
L e 6 T Youngproduced f Q l'Z C)
O l I
l l
a
=
=
1=l
-l 6
Young produced lJq 1
110 l29 1
jc5 Totalyoungproduced l
3 2q
.5 l
3' sic Final Adult Mortality l
l ll-
-Illl Note: Adult mortality (L - live, D - dead) l Concentration:
%Mortality:
.f hl.
I Mean Offspring/Female:
I M. 2..
% Reduction from Control: -1 i'.
CONC: 100%
Survival and Reproduction Data I
Replicate number a
Day jI 2
3 4
S 6
7 1'9 10 A
Young produced O
8 c)
OJ 0 01 llAdult mortauity L-7 2
Young produced l
J l
0 l
b l ( )
=1 1 Adult mortality g
U l
l 3
Young produced l
o l
-0 I
L o
lO I
1 Adult mortallty L
La l
4 Youngproduced l
Q j
(O lI l
rL
-l--
Adult mortality l_l L
-IL U
I Young produced a
a a
I Adult mortality l
l l*+l
.==
a 6
Young produced ol3 l
0 0
° ll
-C)
C)
- 0 Adult mortality l
L L
L-L-
l U
l l
L-
- t.
U.
7 Young produced I IT 2.0 I Ll III IS 6 1'2 h
Jim I I 1i a-a a
a a
a a
a
~ -
Totalyoungproduced ls 3 a3'I a
J l 3 l 3 l 3l Final Adult Mortality a
a a
a I
l a
l a
a I
Note: Adult mortality (L - live, [)
dead)
'*'SPLtT b5='b Concentration:
I i % Mortality:
j CJ70 l Mean Offspring/Female:
L
.3 I
% Reduction from Control:
13.9i l
Envirnnmntal Testiff Solutions, Inc.
Page 5 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant -Non-treated Date:
po CONTROL -2 Survival and Reproduction Data Replicate number Day 2
1 3
4 l
S 617 9
to Young produced C) l 0
Q C)O Adult mortality
(
l I
> J L
J 2
Young produced L
()
Adult mortatity lL-L-lI.(1 3
Young produced l O
L)
Adl mortality L
_I_
L=
_( Ll 4
Young produced 1
ol4 94 lS S
G; S
° T
14l L
Adult mortality g
I Ll LI 1
L S
Young produced 1C1 O
Adult mortality
'-Ii'
- I~
V -
6 Youngproduced L
0 1
1
.O I O O[Ol Adult mortality U
l-lI-l 1
L__
7 Youngproduced ll jlo 1l 1'
l l
IS I
l l 16 1 1 L__
Total youngproduced ze 1
z c
B ao 3I4 T 1 aq Final Adult Mortality L
1
- C7 l l
l l__
Note: Adult mortality (L - live, D - dead)
- . I Concentration:-
I % Mortality:
I ti'?.
l Meaa Of prineg/Fmale:
. 31.3 I
CONC: 100% Intake Survival and Reproduction Data J
Replicate number l_Dayl I
l 2i l
3 4
5 6
7 8
9 l
I I
I Youngproduced 1 O r ZI I
1 Adult mortality l
L l
7 l_
Adult mortalIty=
L L.j
}LL-3 Young produced l
OO O
O
° Adult mortality jL-T II 4 You-g produced s
(p I___I_
L I.
I Adult mortality 1.
5 Youngproduced 1 5
)
I5 1
I 1 5(
I S T 1
Is s.
Ad ult mortality LI-U L
t Uj Ll 6
Youngproduced L1 O
O l
0
=
Adul t mortality L-l L
L L-L E
t T 0
_ou 16 I I -.-
4 e
1 Total Young produced w 2.C 3;
.35 33 I6
==
Final Adult Mortality l
C c
U +/-
l rNote: Adult mortality (L - live, D - dead)
Concentration:
I i% Mortality-.
I CZ.
l Mean Offspring/Female:
135.
j
% Reduction from Control:
- iA.' 7.0
TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Verification of Ceriodaphnla Reproduction Totals Control-i Day 2la unuber 2 l3 5
7 l J8 l 9 l 1 Tdd 1
0 o I o 0 O
O I
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 6
6 5
4 5
6 4
4 4
4 48 5
0 10 13 10 11 10 10 12 11 10 97
- 9 0
0 0
0 0
2 0
0-0 11 7
1415 is 13 16 16 13 12 16 13 146 Total 29 31 36 27 32 32 29 2S 31 2?
32 2 10.98%
Da Re hb Te ntaler 1Y 2
3 4
5 7
t 9
Tis 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 O
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 5
5 4
5 5
5 6
5 5
50 5
10 0
11010 1
120 12 14 s0 0
12 0
10 0
0
_0 11 0
0 33 7
16 Is1 161 15 iIs 14 15 14 156 Toal 31 35 32 36 3
34 31 31 32 33 31 22%
Ds Repltnte nuLlber l
1y 1
2 3
4 05 1
7 0 0
0 0OW 2
0 0
0 0
0I0 0
01 0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 5
5 4
5 14 6
4 5 15 49 9
0 12 1 1 1 1 ]0 rO 11 0
O 0
44 6
0 12 0
0 11 12 12 0
10 10 67 7
19 15 15 17 16 1515 17 19 15 163 Ttd 34 32 32 32 33 311 33l 32 34 30 323 43.9%
Day R4 pelcate nuner T
Tot 1)27 l
1 2
3 4 1 5 1 s l
Total
§ 1
-2 I
0 0
0 0 0 0
0 0
0 0
0 2
0 0
0 0
00 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 6
5 4
4 5
4 4
5 5
5 47 5
0 0
0 01 11 0
10 0
1 1 0
1 32 6
13 10 12 1 10 0
12 0
12 0
121 81 7
1815 17 15 14 18 16 16 17 16 162 Tota 3
36 33 29 36 341 30 33 33 33 322 72%
l a lRe liate number Tel 1 2 3 1 4 1+/- +/-
1 1
0 O
0 0
0 O
0 0
2 0
0 0
0 0
0 0
0 0 10 0
3 0
0 0
0 0
0 0
0 0
0 0
4 85 45 5
65 5
49 5
11 0
10 10 14 11 12 14 10 12 104 6
0 12 0
0 0
0 0
00 0
12 7 114 15 17 19 17 1814 15 19 167 TOWa 35312 321 38 34 35 33 29 3
3 100%
ly Replcte number Total ay I 2
3 4
5 6
7 8
TIta I
o 0
0 0
0 70 O
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 5
6 6
4 4
4 6
5 1 5
50 5
12 0
1 11 13 10 11 11 1
2 82 6
0 137 0
0 0
0 0
0 11 10 34 is20 19 19 1816 16 19 18 178 Ttl 53936 3
32 32 33 32 36 35 344 Control-2 1
0 0-0 0
0 0
0 0
0 0
0 2
0 0
0 1 0 1 0 0
0 0
0
-0 0
3 0
0 0
01 0 0
0 0-0 0
0 6 11I10 12 0 10 0
0 0
0 0
43 7
16 114 14 13 15 14 16 16 17 16 151 TOWa 3
28 30 29 30 30 34 32 34 33 1_313 100% Intake Day
~~Re klate AwnberTO Da 1 2
31 4 5
61 7 8 9 16 oa 1
0 0
0I 0 0
0 0
0 0
0 0
2 0
0 01 0 0
0 0
00 0
0 3
0 0
0 0
0 0
0 0
0 0-0 4
5 6
S 6
5S 6
5 4
5 52 5
0 0
It 12 11 0
14 11 15 13 87 14 HO 0
011 O0 000 39 7 1 2019 17 17 20 18 1918s 16 180 Total 354 53 336 38 35 73 38
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Project number 2220 Reveiwed by: /1 i
t!
Cocetrtin epict nmbr Concentntien Repica umbe Survival Average reproduction Coflcleutof Pmentrnduetle *rot (1) l 2
3 4
5 6
7 8
9 10
()
(offspringlfemle)
PM C()
p ol
)
Control-I 29 31 36 27 32 32 29 28 31 27 100 30.2 9.2 Not applicable 10.98%
31 35 32 30 30 34 31 3.1 32 33 100 31.9 5.2
-5.6 22%
34 32 32 32 33 31 33 32 34 30 100 32.3 3.9
-7.0 43.9%
37 30 33 29 30 34 30 33 33 33 100 32.2 7.6 46 72%
35 31 29 32 38 34 35 33 29 36 100 33.2 9.0
-9.9 I%
35 39 36 34 32 32 33 32 36 35 100 34.4 6.6
.13.9 Control - 2 33 28 30 29 30 30 34 32 34 33 100 31.3 6.9 Not applicable I%
Intake 35 40 35 35 33 36 38 35 37 34 100 35.8 5.7
-14.4 Outfall 101:
Dunnett's MSD value-PMSD:
Intake:
Dunnett's MSD value:
PMSD:
2.362 7.8 1.632 5.2.
MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum pernt difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity testa when a toxicant reduces Ceriodaphnia reproduction by 9.7% from the controt LowerPMSDbounddetaminedbyUSEPA(l10 percentile) -11%.
Upper PMSD bound determined by USEPA (90' percentile) - 37/.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chrnic reference toxicant test.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R4.0-003. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Statistical Analyses Ioiedaphs SurwIM and RIt udtentda Tiet-Reprededia SturtDat:
I1/4200 Ted ID, COdFR Sample ID:
7VAISQN 101,Nonrbald Sad De.
1l230 Lab 1D:
M a-Sa. Teatig SaL Sample 7AW DIOR Dishg odnk g Rpo Sample Dat:
Fetocok FWCHIR-EPA421.R02.013 Test Species:
CDCubdapbak dub-a C
bmm C e.%
I 2
3 4
S 7
S 9
to D-Ceatrol 29.000 31.000 34000 27.000 31000 3.000 29.000 23.000 31.000 27.000 1033 33.000 35.000 32.000 30.000 30.000 34.000 31.000 31.000 32.oo 33.000 22 34.000 32000 32.000 3.000 33.000 31.000 33.000 32000 34.000 30.000 43.9 37.000 300o 33.000 2i.oo0 30.0oo 34.000 30.000 33.0O 33.000 33.000 72 35.000 31.00 29.000 32.000 31.000 34.000 35.000 33.00 29.000 36.000 too 35.000 39.0O0 36.000 34.000 32000 3.000 33.000 32.000 34.000 35.000 T
rifem: Untuutermed
.-Tafled sateak COea-%
MMeU N-Msa w
Ma 11le Mar CV%
N 0Seat Citica MSD Mea N-Mes D-Cakol 30.20O 1.000 30.20 27.000 3OO 9.206 30 32367 LO1OO 10os 3.900 1.0563 31.900 30.000 3s.000 5.214 10
.1.645 22127 2.362 32.37 LOO0O 22 32300 1.0695 32.300 30.00 34.000 3.175 10
-2.033 2.27 2362 3234 LOD0 43.9 32.200 1.0662 32.200 2i.000 37.000 7579 10
.136 2.297 2.362 32367 1.0000 72 33.200 1.0093 33.2DD 29.000 31.000 335S 10
- 2sc4 2.27 2.362 32367 L.OO0 300 34.400 1.1391 34.400 32.000 39.000 6.601 10
-4.065 2.237 2132 32367 1.0000 AcxiacTab Stetld Crltiesl skew KSbt KolmogazovDTut ledioetes -1 adistiudan > 0.01) a.714224041 1.035 a403227399 0.064324151 Btetka Teat Indiates eovalverlme (y 0.1- )
8.037992477 15.05627224 Ifbyptes Tat I L.6 NOEC LOEC CV TU AMIB KSDp Mse MSK F-Nrb dn Duanatt Teat 100
>100 1
236247902 0.076277Z2 1956667 5337037037 0.006318601 5,54 Thatmeflt v D.Cootwl Lsar Interpeitlso (200 Reseples)
Peout
- A SD 95% CL Skew IC05
>100 2C10
>100 ECls
>100 1.0 2C20
>100 03.
IC25
>100 0.a I750
>100 0.7 0.6
}0.4.
0.3 0.2 0.0 40.1 0
10 40 so so 100 120 Dos %
Des~epemeFlt 42 y 40 35 30I25~
20 Is, 10' S.
x 14-k 0.06 lAI of signfcarce vi-I a
0 ea 8
xqn i-6-5da
TVA / Sequoyah Nuclear Plant, Intake Non-treated November 16-23, 2005 Statistical Analyses Carliodaphk Sarvlvd aid Ptepredta Tat-Repredwedoo Stft Da.
IU161200$
Test ID' CtdPR Saimple ID, TVAJ SQN Intalme Nan-teated REd Date IM2M123005 LAb Mt M1a-Rl:
- w.
lgSO S
Spk1mp D
io-Db Multaft Paport Sample Dae:
hatocok FWCHMA t21t-R.2-013 Tet Species:
CDCeedaphuk debiL Cae-%
1 2
3 4
S 7
l a 10 D
ol 33.000 2S.OD 30.000 29.0DD 30.000 30.000 34.0D0 32000 34.CC0 33.000 1CO 35.000 40.000 35.0DD 35.000 33.0D0 36.0D0 31.00D 35.0DC 37.CDD 34.000 Tmateui? Uatmmsteermed 1-Taile b
kete Cosle-%
MOK
-Mdesx MM MLL Mar CV%
N tutat CdCal MSD Mm 14Me D-COMko 31.300 210 31.30 2.0DD 34.00D 6.910 10 33.550 I.ODOD 100 35.t00 1.1438 35.300 33.000 40.000 5.709 10
.4.7D2 1.734 1.632 33.550 1.0000 Aulilkry Teseb Statistic Crltcal Skew Knrt Sbpko.WlL Teat dlkats -
-Ia ditsbuthat (p> 0.0) 0.953691359 0363 0.35017145 -071197525 F-Test tes equal variences (p a O.37) 1.119620112 6.541099535 Ityidhla Tet(1.all.0.
MSDu MSDp MSB MSE Fb dt Hamoscedaste;tTetkdicatee so sWffens dlffcnnees 1.63122565 0.052134906 101.25 4.427777M 1.5h-04 1,11 Mamw s D.Coutool LieaerlIterpolatio (200 Rwassplet) bat SD ffCL Skew 3005
>100 ICC5
>100 ICIO
>100 1.0 IC2i
>100 0.9 IC25
>100 0.3 IC40
>100 0.7 IC50
>I00 0.O 05I 0.4 0.3 02 0.1 0.0
.0.2 0
20 40 50 SO 100 120 Dose %
Dla~a.apase" plot T
40 35 30I25 20 15 10 5'-
14af, 0.06 lewl of igrnlllcance LI
- sqn 11-16-05data
Page I of 6 Environmental Testins! Solutions. Inc.
Environmental Testin2 Solutions, Inc.
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Seqiuoyab Nuclear Plant NPDES #: TN 0026450 Project #:
Zz.Cn County: Hamilton Treatment: UV-treated Outfall: 101 Diludon prepara ion information:
Comments:
Dilution prep (%)
10.98 22 43.9 7
100 Each concentration was treated Effluent volume 274.5 550 1097.5 1800 2500 for 2 minutes with a UV sterilizer (mL)
Diluent volume (mL) 2225.S 1950 1402.5 700 0
to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences.
Test organism informadon:
Test information:
Organism age:
22.Si -rio z4.S 4*oZ5 o~ b RandoZink template:
6Ltb Date and times organisms n.ts-Os
%3J T
t¶Q)o Incubatornumber:
were bom between:
Organism source:
Ae6 A-ria Nr V z
S-o5 Artemia lot number.
86&%tNT Transfer bowl information:
pH =
Temperature -
°C Total drying time:
Z_____S
&- It
>I.7 Date/ Time in: 11-2z.b tb50 Average transfer volume:
Li.4 Date / Time out: Il -2*
i 3st56 Oven temperature:
- t. 6c' Dailyfeeding and renewal Information:
Day Date Morning Afternoon Test Initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0
1-.
-4 ISOO i3qLI l-il-A OSl05ilS.cA%
lI-it -056 0Cd7..
%S7o a___
i1-c86a r.o, c; I
i2 I%-
s 0l4s s,
1 2.
i-11-OA B 1114. 1.
LI _
4
-1.0os t
It-zo oS
?-IS l
s i 1-S
-A lbIII I
3 d 11-2j -oS OqO*
lSI 1 1 I
0- 11-OS b zImug.z
'41 3 6
11-t2 i1ts2 05 IMq. lz24. 1 0 A
-t I
7 11 2Iz5-csFRINVOM 137tZ
%a b-KEK Control information:
Acceptance criteria Summary of teSt entoomts
% Mortality:
M (s
20%/9 7-day LC5
> I_ ___
Average weight per initial larvae:
IO. (C0(
NOEC 1007',
Average weight per surviving larvae:
T. (0 ( 02 in ae LOEC lI 7
[ChV
>,cvy&,
IC23 J
i 07!
Envrironmental Testine Solutions, Inc.
Page 2 of 6 Environmental Testin2 Solutions, Inc.
Page 2 of 6 Species: Pimephalespromelas Date:
I l-1b-oS Client: TVA / Sequoyah Nuclear Plant - UV-treated Survval and Growth Data Day CONTROL 10.98%
22%
A B
C D
E F
I G H
I l
Jl L
o0
/0 (0
tO 10 IO 10 Io 10
/0 10 3
110 I/O/0 t
o t't 0/0
/00 IO I
10 1C 10 1/0 lO
/l 1
1
/0 1
/
1 IO 10 2
/D)0 o lo lo lo O LO tO 10 I1 I 10 /O 1010 0 to lo
/0
/0 to
/
Ito /I0
/0 7
}()G 1
t, 1G Ur KA O
~
O O
l 6
0>
- 11)
I0 Jo)
I lb 1 IG-. /
/ IC) 0G/0 X
7-
-O
-O
-O
-O 1
O A - Pan weight (mg)
I9. 3 Color identification:
IELB.L 11.CZ 16.02 tS.o¶ 16.oo l6.o2 14.0 Analyst:
L t.73 i6. 28 16.21 B - Pan + Larvae weight selyW f
2 00i 21.0o 21.br 1 z LOZ 2zo2.5 2
22q Rlqj zIDo lZ.*'
2zts 2a6#40 Larve weight (mg) - A - B L.X11 8
.-o n.3Z7 t.S6 5S.St s.4l1
!584.ZI3
.11 Weight per Inttlal number of larvae (mg)
- C / Initial number of larvae 4'
0*
O0 oN
%0 V0
'V Q.b V
0 O-4.
0*
43
\\0 0D
\\9
\\'Il 01 DI Avernge Percent weight per reduction Initial from entrulI
- 0. (o unumber*t
(%)o larvje (Mg)
I -
I. -
J.
i -
0.'ovZ-S.A7.
Comment cde: c - clear, d d dead, fg - fungus, k-killed, m - missing, sk - sick, sm - unusually small, Ig - unusually large, dMr - decanted and returned, w - wounded.
.A Calculations and data rcvivwec L Comments:
Page 3 of 6 Environmental TestinM Solutions. Inc.
Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
IH6-aS Survival and Growth Data Day 43.9%
72%
100%
M N
0 P
Q R I S T
U V
I W X
1 0 1C1
/0 0
it J 1 0 I6 1 0 1 0 1 0 Q IO IC)1 10 I0 10 1 0 IC0 I/ 10 10o
/0 2
/0IC)-/0
-/O IC) 1010
/1 0/0/
/0 J2 LO 10 16
/O 10
/Q I) lo
/0
/0. /O 1 010o10 100 O 1 0 0 10 10 10 10 2
10 10 10 Jo 1
I 0 /O
/00 /0 10 10 6
(0 l
/0
/O
/l IC/ /O to
/O
/'0 io
/0 pD 10 tO 10 10 10 tO I(.
(0 lO 1 0 (0 D O to to lo IO 10 1d tOO IO APan welght (mg) 1V15 1
Color Identification:
1 1_.
IL&4A52 16.37 16.21 L7617 Analyst:
J 15.17 1.73 B - Pan + Larvae weight Analyst: I*)(ATw4d, 20.00 22.jq
,3.8f 21.55 21.Z3 25 1S 20.GZ 2ZAA 2zoM 2 1.1" I6 ZOJ Larvae weight (mg) - A - B Weight per initial number of larvae (mg)
~
~
C / Initial number of iarvae
£7 9
A.
'7
'0
'0 O-
-O-a' O
O-a>
C-
-O Average Percent weight per reduction number of (V/)
Comment codes: c - clear, d - dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r = decanted and returned, w - wounded.
Calculations and data reviewed:
Comments:
Environmental Testina Solutions. Inc.
Page 40of6 Endrnnnientftl Tetin nh1tionL Inc.
Page 4 of 6 Species: Pimephakespromelas Client: TVA J Sequoyah Nuclear Plant - UV-treated Date:
Il-1I6-Survival and Growth Data Day 100% Intake Y
Z AA BB 0
10 1
010 1t 10 ICO 10 21
/C) lo /O
/1
/
/O
/0 41O
/ lO 10 to__
_Ito0 1
ib 6
/0 10 /0 10 7
10 P/O I/() /
A - Pan weight (Ing) 15.02 Colorldentification:
L~~e2~~L I'.7 If83 Analyst:
J 6
B - Pan + Larvae weight (mg)
- lrkg, Analyst:
1&
1 Zl V D>
Zi.01 2ebk5 Larvae weight (mg) - A - B Weight per initial number of larvae (mg) r A
C / tntdalI umber of larvae O
Average Percent weight per reduction S6 0L Initial from control 0"'
number of
(% )
Comment codes: c clear, d = dead, fg - fungus, k - killed, m = missing, sk - sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.
Calculations and data reviewed:
Commentss:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Reveiwed br.
/Y Project number:
2220 Not for Complitnce Assessment. Internal Laboratory OC Comeattle.
R(. teptke tnwilal number.1 totr I.B.-ber.1 Aof A
'a. wdgt 3
sn.La, Lae wih1mg t I-S S gmIvb g
Mean weigdt I Cuefdene vaerliato Weigtbil tial mamber t servial ean weighmib CIlfldeek of Perat redweti I Nm
- aoea, areae (mt) wdghtl (
-A.B uamberdbtensetmg)
SarYlf-g berd 0__"dPa dftretma)
(%)
taktnanaherol vareatka &
du moate
)
larve (NW I~i(~
hrvaetma
,..Mme..e..h-o A
10 10 14.92 20.54 5.62 0.562 0.562 Ceta I20 I10 14.62 21.80 7.18 0.71880.
0.718 CCntrl I0 10 14.73 21.61 6.88 0.688 0.660 10.3 0.688 00.0 0.660 10.3 Not appliable i I0 10 16.02 22.72 6.70 0.670 0.670 E
10 10 14.70 22.02 7.32 0.732 0.732 10.98%
F I 0 1O 25.09 22.65 6.56 0.656 0.637 12.0 0.656 97.5 0.622 14.4 S.
G I20 2 0 26.28 22.79 5.52 0.551 0.552I II 210 9
6.00 21.47 5.47 0.608 0.547 I
10 10 14.63 21.01 6.38 0.638 0.638 22%
K 10 10 26.02 22.44 6.42 0.642 0.622 5.5 0.642 380.0 0.622 55 5.6 K2I0 2 0 16.21 22.59 6.38 0.638 0.622062300
.2 L
20 10 14.69 20.40 5.71 0.571 0.571 M
I 0 2 0 14.75 20.00 5.25 0.525 0.525 43.9%
N I10 10 16.12 22.14 6.02 0.602 0.617 10..0 07602 100.0 0.617 1.0 6.5 o
io 2 0 16.08 23.84 7.76 0.776 0.2 800.776 8000671.
P 10 10 15.92 21.55 5.63 0.563 0.563 7
20 20 15.06 21.23 6.17 0.617 0.617 R2 I
20 2 0 16.37 23.15 6.78 0.678
.257 0.678
- 0.
.2 S
I0 2 0 15.17 20.82 5.65 0.565 0.625 7.3 0.565 100.0 0.625 75 5.3 T
20 10 16.29 22.67 6.38 0.638 0.638 1
U 20 10 14.82 20.99 6.17 0.617 0.617 100%
V I20 210 14.78 21.68 6.90 0.690 0.621 8.4 0.690 100.0 0.621 8.4 5.9 2 0 2 0 14.73 20.84 6.11 0.611 0.611 X
20 10 14.97 20.61 5.64 0.564 0.564 V
10 10 15.02 21.04 6.02 0.602 0.602 100% Intake Z
20 10 14.87 22.05 6.18 0.618 0605 3.1 0.618 100.0 0.605 3.1 8.2 AA 10 10 14.66 20.87 6.220 0.62 0.620 1111 20 20
- 4. 320.63 5.80 0.580 0.5 0_
outfall lee:
Dunnett's MSD value:
PMSD:
Intake:
Duntett's MSD value:
PMSD:
0.1224 13.6 0.0685 10.4 MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of teat precision. The PMSD is the minimum percent difference between the control and treatment that can be declined statistically significant in a whole effluent toxicity test On averaM a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 14.0% fins the control (determined through reftrence toxicant testing).
Lower PMSD bound detennined by USEPA (lOth percentile) 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) - 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-433-R0003. US Environmental Protection Agency, Cincinnati, OH.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated November 16-23,2005 Statistical Analyses 1-1 FIbA d
S-I-T-t7 I-CY-StAt Dste.
Il/62C05 Tea ID:
PPFRCR Sample ID:
TVA/ SQN 101. W.Uaated Rad Dss 11//2205 Lab W.2 MakETaftli Sol SaVmpe 7ypc DMR-Diarp Monkrkg Rqmt Saple Dalt:
rnitocok FWCHR-?A,421.R,02-013 Teat Specks PP-Pipnapaks Fweh C~ac%
1 2
3 4
D0Ccfolo 0.5620 0.7180 0.6100 0.6700 10.91 0.7320 0.560 05510 0.5470 22 63tO 0.6420 0.6310 0571O 43.9 0.5250 0.6020 0.7760 05630 72 0.6170 0.670 035650 0.6380 100 0.6170 0.69O0 0.6110 0.5640 Traufartsa m: troafomed I-Towed Isateak Cec-%
Mena NMes Mm KlIl Ws CV%
N
$.Stat Cdl MSD Ma NM D-Coantol 0.6595
.ODD0 0.6595 0.5620 0.7180 10303 4
0.6595 1.OXO 10.98 0.6215 0.9424 0.6211 0.5470 0.7320 14361 4
0.74t Z410 0.1224 0.6219 0Q94D 22 0.6223 0Q435 0.6223 0.5710 0.6420 5A99 4
0.733 1410 0.1224 0.6219 0Q94D 433 0.6165 09341 0.6165 05250 0.7760 17Mi6 4
0.46 2410 0.1224 0.62C5 0.94C 72 0.6245 9469 0.6245 Q5650 0.67S0 7.534 4
0.6t9 1410 0.1224 0.6205 0.9409 100 0.6205 0.9409 0.6205 0.5640 0.6900 1.3 4
.761 2.410 0.1224 0.6205 9409 AsaxIIyTub Sta&&
Qridcal Skae Kart SbApka-Wkl Teatklicat s id al dibutip>O.O1) 0951492696 0.814 0.561262513 0.354520295 B&*
eS t indicates equlv uecs (p -0.45) 4.741465045 15.03627224 Rwap esk Test(1-e4iLOS)
NOEC LOEC OkV TU MSD*
MSDp MS1 bMSE P1? b 4f Dunmare Tet 100
>100 1
0.122449555 0.15670283 0.001013242 0.005163097 0.59911439 5,1t Sicastents v D-Ccurol Linarlaterpladtio (200 Raampls)
PFat SD 9S%aGCZP)
Skew ID5' 9.6230 ICIO
>100 IC15
>100 1.0 IC20
>100 IC25
>100
° IC40
>100 0A
- idicatae IC estknate I tan tle bweost encentation I0.6
[05 0.3 0.2 0.1 0.0
-.... <,<. ~. ~
0 20 40 60 s0 100 120 Do Drea-Rtapowe Pelot 03, 2 0.6 0.7 0.5 CA 90.3 0.2 0.1 I
I I
.L
-a-. I4all.0.5 levAl of at gnificamec I
a 9
8 sqJ)-)6-05data
TVA / Sequoyah Nuclear Plant, Intake UV-treated November 16-23, 2005 Statistical Analyses la1 ! fish Grnwwt md Svil Twst Day Gewtk Stutt WC LI16J2C0 TetD:
PpPFRIR Sample 1D TVA/SQN Ilak WU-Iated Ed Dae:
IUI312C05 Lab ID:
i-Bmvk/.TwtkgSoL Sample 2wpe DMR.Dbagc Mon gBt pct Sample Dte:
Potcot PWCHFR3PA2W-RO2N013 Tsdt SCier:
IT-p?
phuks Froen8k Commeats:
Coae.%
1 2
3 4
DC ol 0.5620 0.7120 0.6t0 06700 100 0.6020 0.6120 0.6210 00 Tnmsfu Unbsutorm"d I-TAWld lbtk Cme.%
Me"a N.Mesu Mets MIN Mal CV%
N t1tat ads MSD Me N-Mea D-Cmbol 0.6595 10020 Q.6595 0.9420 0.7180 10.303 4
0.6595 L.O00 100 0(6053 0.9177 0.6053 0200 0.6210 3.104 4
1.539 1.943 os5 Oh6053 09177 Axiliary Te 6utltde Clet Skew Kurt Sh9aiy-WIk Test Judidcls n s distbutin ( > 0.01) 0.224777725 0.749
-t.3206049 2902723251 P-Test ;
eqe nsl (p - 0.06) 13.02O40191 47.46722794 Nypobsl Tet tl.l.
Idl)
MSDv MShMp.
MSB F=
7-Prob dt HomNeeutc Ted Itdiew so gpuitima diffmm 0.062494209 0.103252695 oLOO5SS6125 0,002414953 0.174713352 1.6 Thatmenft v D.Cmtfrl LUerlIterpoldstn (200 Rtnampleo)
POW SD 95% C.(rxp)
Skew ICMOS 60.723 lC10
>IX0 IC15
>100 1.0 IC20
>IOD 020>100 0.9 IC25
>100 as 100
>100 06 IC5O
>100 0.7 k~dieat IC etnatt less II=n dt lowest anentatiolk 0oe 0.5 1 0.4 0.3 0.2 0.0 0
20 40 s0 s0 100 120 Dos %
DesRapeasePlat e.7 O
14al, 0.05bd J*of algrincanre 0 0.4 903 0.2 ILID sqn_ 1-1 6-05data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Project number:
2220 Reviewed by:
Ck4-Concentration Parameter Initla RH (SU) 7 DO (mx/L)
Conductivity (,tmhos/cm)
Control Alkalinity (mglL CaC03)
Hardness (mg/L CaCO3 )
Temperature CC) 2 2
I 01 81 VI nrH (SU) 4-DO (mulL' 8.00 8.00 8.10 7.77 8.03j 7.801 8.031 7.871 8.021 7.871 8.051 7.62 7.9 7.5J 7.6 7.21 78 8.0 7.4 8.0 7.5 7.7 6.9 300 303
_I 292 30c 29 288 10.98%
4-Conductivity (iimhos/cm)
Temperature (C) 25.1 24.9 24.J 24.41 24.91 24.1 FI 25.
24-25-24-1 24 RI 24.
24.R 24.7 t
I--
4.
pH (NU) 7.9; 7.871 8.0 7.9 8.1 0I 7.731 8.0 7.8 8.0 31 7.8, 8.051 7.66 22%
DO (mg/L) 1 7.4 7.5 7.81 7.61
.7 6.91 7.71 7.6 7.3c, 71 7.9
- 7.
786 7
Conductivity v(mhos/cm) 24 2841.
281 2E 2
276_
288 28 276 Temnerature (e) 25.
24.9 24.9 24.
24 91 24' 71
- 25. I
?A 4 7I7ZI
4 ii -4 -4
*-*
.4
- .4
- i*
;4
*;4
*J
pH (SU) 7.9; 8.0(
7.9 8.1I I 7.7'91 80(
78 43.9%
DO (mn/L) 75 74 7.7 73 7
[71 76I 75 il Conductivity (Pmhos/cm)
I 256 4_
259J._I 270i 250 260 _
Temperature (C) 25.
24.
24.9 24.(
~91 pH (SU) 7 94797 790 801 7 9i 72%
DO (mgIL) 7.5 73 78 7 5 Conductivity (Pmhostcm) 219 223 Temperature CC) 25.1 247 250 246
.4-'
.4-
.4-
.8. -
7 1mm nH ('SIn 7951 788 R021 79i DO (m/L) 7 I7 I77
^(?onduetiviNv {stmhor/cm)
.vatuswtstaws tp--X--vvaEz 100%
Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO3)
Total Residual Chlorine (mg/L)I
<0.
Temnerature (el 25.11 24.71 25.01 24.6 7.84 8.
7.5 V
24.7 24 7.87 8.
7.5
<I0 I
4717Z7 I
-.1
.. J Till I ill pH (SU) 7.941 7.881 8.011 7.971
8 DO (mdL)
-I-xndwsrf1z4tv {somh^elem bunuuctesty t>tIUIUSRV I
100% Intake Alkalinity (mg/L CaCO3)
- 8-Hardness (mglL CaCO3)
Total Residual Chlorine (mg/L)I
<0 Temneratur, (C)1
.5
Environmental Testina Solutions. Inc.
Page 5 of 6 Environmental Testin2 Solutions. Inc.
Page 5 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Daffy Chem-st :
Date: 1U-1o-S I
Day I
0 1
(y 2
Analyst RtM I A46 IAi5114 I fL4i5 I
R~J JN-Concentration I Parameter CONTROL nH (S.U.)
'8 i
'.q92 1 4.q9 I 0.02 I 1a65A 1.8G
\\_.
u u
DO (mg/L)
L tt Conductivity (umhos/cm)
-i7
- x,....__4 Alkalinity (mg CaCOi/L)
.4 Hardness (mg CaC0SL)
\\-
- -=
r Temperature (°C) pH (S.U.)
X§ 10.98%
DO (mg/L) q Conductivity Zq (Amhos/cm)
Temperature (°C)
A5) pH (S. U.)
0-~31 22%
D m
)
Conductivity 2zs (amhos/cm)
Temperature (°C)
_ t.
b1 2S.()
>.W 5
1 7.
I 0.1D 7
-73 1
pH (S.U.)
43.9%
DO (mg/L)
Conductivity
_(umhos/cm) a Temnerature (°C)
PR (S.U.)
72%
DO (&)
7.5 Conductivity 219 (umhos/cm)
Temperature (°C)
.1 1H (S.U.)
100%
DO (mgtL) t5 Conductivity 163 (gmhos/cm)
Alkalinity (mg CaCOJL1) o Hardness (mg CaCO3/L) 1_
TR chlorine Temperature (°C) 5.1 7.1&
1 I 1 BAe
~
91.7l.
7 1
2+0-
-.0 Z4.
I Rlo I.2 I ZSl_
M 4U., 0.7 1 2S. 7
-S-pI (S.U.)
\\
I 100% Intake DO (mg/L)
Conductivity (uimhos/cm)
Alkalinity (mg CaCOWL)
-I.
Hardness I
(mT CaC/L) o7 ri (mglL) l i
1 2q.
1 Iniia Temperature (C)
I P.
Environmental Testing Solutions-Inc.
Page 6 of 6 Environmental Testirn Solutions. Inc.
Page 6 of 6 Species: Pimephatespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date:
tk-l1-OS I
Day 3I 3
I 4
I S
I 6
I a
Ia Analvit Concen-Parameter tratlon I
CONTROL MhS pH (S.U.)
DO (mg/L)
I,-
~
Conductivity (ulmhos/cm) a, _
Alkalinity (mg CaCO1 L)
Hardness (mg CaCOV/L)
.1
_17-Temperature (C)
PH (S.U.)
6 5 10.98%
DO'(mg/L) 7 8 Conductivity z
2 t(gmhoslcm)
Temperature C) 2
\\
ZUI pH (SU).)
22%
DO (mg/L) 7.?
Conductivity 7
v (4mhos/cm)
Temperature (0C)
.0 23 pR (S:U.)
43.9%
DO (mg/L) 7.
Conductivity l---
(Amhos/cm)
Temperature (C)
-s.
- 2.
2' pH (S.U.)
7.'?
7.
72%
DO (mg(L) 7.6 Conductivity 21 Z (gmhos/cm)
Temperature (°C)
-Zs 2
pH (S:U.)
100%
DO (mg/L)
=
Conductivity (umhos/cm)
\\ r- -- -_ _ _ _ _ _ _ _ \\
Alkalinity (mg CaCO./L)
\\---c Hardness (me CaCOSL-5...D ___ _ P _,
Temperature (C)
V-pH (S.U.)
r-w-
100%
Intake DO (rng/L)
Conductivity (gmhos/cm)
Alkalinity (mg CaCOA)
_71
-1 9.
- i 7 3 lE Fina
- L'2.
9i'4*
- 1. Zi :
Z1.1 I_
,03;c~e iiiLeL Hardness (mg CaCOVtL)
Temneraturo t0 cr I
n it i I I
F i n a n
I t a
.F i n l I.
Initil
_1 FinalI Initial I
Final I
,TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dutbia Daily Chemical Analyses Project number 2220 Reviewed by: _W&
- Concentration Parameter D14 (SUM DO ImvJL)
(>ondsetivitv lemha/rm}
vE7--~lZ-vllJt~zll'77...I Control Alkalinity (mgiL CaCOl)
Hardness (mg/L CaCO.J T.mnerst..-r tf
- ?A.
,2 0 4.91 pHl(SU) 7.92 7.96 8.00 8.03 8.10 8.09 8.03 801 8.03 7.9 8.02 8.03 8.05 8.01 DO (mg/L) 7.4 7.5 7.9 7.9 7.6 8
7.8 78 8.0
- 8.
8.0 8.0 7.7 78 10.98 Conductivity (iimhostcm) 291 300 303 292 300 292 28'3 Temperature (C) 24.9 25 24.9 25.3 24.8 252 25.0 251 24.8 25.2 24.8 24.9 247 25.3 pll (SU) 7.93 799 8.00 8.01 8.10 811 8.02 800 8.04 7.9 8.03 8.0 805 8.05 22%
DO (mWlL) 7.4 7
7.8 7.9 7.7 83 7.7 78 7.9 8.0 7
7.9 78 7.9 Conductivity (pmhos/cm) 284 281 287 276_
28 i
28 27
_Temperature
(-C) 24.81 25.2 24.9 25.2 24.9 25.2 24.9 25.1 25.0 25, 24.8 2
4 247 254 nil tIlU 7.9 7.Q 8.0 8.0 R81 8.1 I 8.01 8.0 8.0 8.001 8.0 8.0 8.U6 43.9%
DO (mg/L) 7.5
- 7.
7.7
- 8.
7.6 8.3 7.6 7.7 7.9 7.1 79 7.81 7.
Conductivity (pmhos/cm) 1 2565 j
259 27t 250 _.
260 4'
253:
w 248 _
Temnerature t-C 24.8 25.
24.
25.
25.1 25.2 24.91 25.1 4.91 4
4
-4I.
t
-I nil IQll 7 9A 7 (
8.0 8.1 X 14
- 7.
- 8.
K0
.UUl 72%
)O (mg/IL) 7 7.5 7.51 7.8l 80 76 81l 7.6 78 78 7.8 Conductivity (,mhoslcm) 219 2
23 21, 27 212?1 l_
222 I'empersture (I)
I.
)11 (SU) 1
DO (mrdL)
Cnnsl*letivitv tumhos/eml
- u-suus-v--^
tu-l---vxsWaw 100%
Ikalinitv (mgIL CaCOy)
Hardness (mg/L CaCOA r'otal Residual Chlorine (mAIL)
<0.
remperatur (TC) 4..
-I A.
A n
A.
nil (SUl 8.091 8.131 7.951 8.041 I-DO (mp/L) 100% Intake zon*UktIIVI c
l I
Alkalinity (mg/I, CaCO,)
hardness (mg/L CaCO 3)
I 183 76 24.I_9 24.9 25=2.
- 4.
48 2.
ilotal Residual Chlorine (mg/L) I Temperature (TC)
Environmental-Testing Sluions, Inc.
Page 6 of 7 Species: Ccriodaphnia dabia Client: Sequovah Nuclear Plant - Non-treated flaili, ChAmhfrv Date:
I-l16-OS t
I I
Day Analnst Concentration rParameter CONTROL pH (S.U.)
DO (mc/Ll
\\
Conductivity (jimhos/cm)
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO4L)
Temperature (°C) tFH (S.U.)
I I
10.984 DO (ma/L)
Conductivity (amhos/cm)
, [
Temperature (°C) pH (S.U.)
1.^3 22%
DO (mg/L)
-+"I Conductivity 2Uq (gmhds/cm)
Temperature (°C) jq.6 pH (S.U.)
9§ 43.9%
DO (mgL) 7.5 Conductivity 251 (gmhos/cm)
Temperature (°C)
,t.
_.L
~.
a3 pH (S.U.)
72%
DO (mg/L)
Conductivity (aimhos/cm) 2'.
Temperature (C)
\\_._.,
100%
i I DO (mx/L)
Conductivity (umhos/cm)
Alkalinity (mg CaCOJ/L)
Hardness (mg CaCO,/L)
-I-Temperature (C) pH (S.U.)
100% Intake DO (mg/L)
Conductivity (timhos/cm)
Alkalinity (mg CaCOA/L)
Hardness (mg CaCO3/L)
_ Temperature (IC) 6.0q AN 1 T?
U1
-3 Z-4040 Initial Final Inid" Final Initia
Environmental Testing Solutions. Inc.
Page 7 of 7 Environmenta Tectin
_nifns In.Pge7o Species: Ceriodaphnia dubia Client: Seauoyah Nuclear Plant - Non-treated Date:
/1-16 bS I
Day I
3 I
4 I
5
-[----4
-4 Analyst I Concen-Parameter tration I
CONTROL n145 pH (S.U.)
DO (mg/L)
.]
Conductivity (umhos/cm)
\\,-
Alkalinity (mg CaCOIL)
Hardness (mg CaCO3/L)
Temperature (°C) pH (S.U.)
8.63 10.98%
DO (mg'L)
- 7. l Conductivity (Ilmhos/cm)
M 2
l Temperature (°C)
'5.0 pH (S.U.)
8.0-L 22%
DO (mg/L) 7.7 Conductivity z
(pmhos/cm) z 1 Temperature (°C T1*9 DH (S.U.)
\\
43.9%
DO (mg/L)
Conductivity
? 50 (gimhos/m Temoerature (C) pH (S.U.)
- 7.
It 72%
DO (mg/L)
- 7.
Conductlvity (lmhoslcm) zW
_Temperature (DC)
Tl 5
pffSS.U.)
100%
DO`4mg/L)
Conductivity (gmhos/cm)
Alkalinity (mg CaCOJL)
Hardness (mg CCOaIL)
Temperature (C) ws M
R U.
P 100%1 Intake pH (S.U.1 t
I C5.0 ZF 1 I "D I 8-o-4
&o3 -6ell 0.0 1
I 8 1 DO (mg/L)
'7.7 Conductivity (umhos/cm)
Alkalinity (mg CaCOJL)
--2! ___-r
(o3q-Final 7
ntal FnlInitial F
Hardness (mg CaCO3/L)
Temperature (C)
-b L
TVA / Sequoyah Nuclear Plant, Outfall 101 U V-treated November 16-23, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Project number:
2220 Reviewed by: A Concentration Parameter D
0 D
I D
2 D
Da 4 D
5 D
6 Initial Final Initial Final Initial Final Initial Fn Initial Flital Initial Final Initial Find pH (SU) 7.92 7.91 7.96 7.98 8.11 7.74 8.02 7.85 8.04 7.85 7.99 7.81 8.02 7.66 Control DO (rniL) 7.8 7.5 7.6 7.5 7.6 7.3 7.9 7.7 7.4 7.9 7.3 7.8 7
Conductivity (iumhos/cm) 305 314 311 30 305 310 290 Temperature (C) 25.0 24.8 24.9 24.6 24.9 24.8 25.1 24.9 249 24.8 24.9 24.9 24.9 pH (SU) 7.92 7.91 7.96 7.96 8.12 777 8.02 7.86 8.05 7.87 7.99 7.81 8.05 7.70 10.98%
DO (mF/L) 7.7 7.5 7.6 7.4 7.6 7.9 7.8 7.4 8.0 70 7.8 7.0 Conductivity (pmhou/cm) 294 293 3044 3(
305 296 289 Temperature (CC) 25.2 24.9 25.1 24.6 25.1 249 25.2 24.9 25.1 24 25.0 25.2 25.0 24 pH (SU) 7.93 7.92 7.97 7.98 8.12 786 8.02 7.89 8.06 78 7.98 7.91 805 766 22%
DO (mzfL) 7.7 7.6 7.6 7.5 7.5 76 7.9 7.69 7.9 7.0 78 68 Conductivity (pmhosucm) 279 279 289 28 291 283 72i5 Temperature (IC) 25.2 249 25.1 24.6 25.1 249 25.3 25.1 25.1 24.9 24.9 25.1 250 24.8 pH (SU) 7.93 7.98 7.98 8.12 779 8.02 7.86 8.06 7.85 7.99 7.9 8.05 7.65 43.9%
DO (mn/L) 7.7
- 7. 7 74 7.6 74 7.9 7.5 7.9 7.3 7.8 7.4 78 68 Conductivity (pmhostm) 254 256 274 2559 _
261 256
_24 Temperature (IC) 25.2 249 25.1 24.5 25.2 247 25.2 24.8 25.1 24.9 24.9 24.7 25.1 24.8 pH (SU) 7-94 7.94 7.99 8.02 8.12 779 8.00 7.87 8.07 7.87 7.99 7.89 8.06 7.71 72%
DO (mWL) 7.7 7.5 7.7 7.4 7.6 72 7.9 7A 7.9 7.3 7.8 7.4 7.8 6.9 Conductivity (pmhosfm) 217 221 224 220 224
_220
_2 Temperature(CC) 25.3 248 25.2 24 25.2 249 25.1 24.8 25.0 249 24.9 24.8 25.1 24.8 pH (SU) 7.94 74 7.99 80 8.12 773 7.99 7.86 8.07 789 8.00 7.93 806 774 lOOY DO (OgL) 7.8 76 7.8 7
7.7 0
7 78 7.9 7.2 7.8 7.1 1
Conductivity (pmhostem) 17 18 E8 1813 183 186 183 Temperature CC) 25.3 24.7 25.2 24.3 25.2 24.6 25.2 24.8 25.2 251 24.9 24.8 25.0 25.0
.H (SU) 7.95 8.01 7.90 8.02 8.12 7.98 7.89 8.08 7.98 8.05 7.88 8.0 7.7 100%Intake DO (nWL) 7.8 7.8 75 7.5 7.6 7.2 8.3 7.5 7.9 7A 7.8 7.4 79 72 Conductivity (Pmhos/cm) 1' 179 176 181 180 184 182 Temperature (C) 25.4 24.S 25.0 24.5 25.0 24.8 25.2 249 25.1 25.1 24.9 25.0 249 248
Environmental Testingz Solutions. Inc.
Page 5 of 6 Environmental Testin2 Solutions. Inc.
PageS of 6 Species: Pimephalespromekas Client: TVA / Sequoyah Nuclear Plant - UV-treated Dailv Chemistry:
Date:
//-I4-05*
I Day I
Analyst Concentration I Parameter CONTROL MA Rv3 pH (S.U.)
DO (mg/L)
Conductivity (Pmhos/cm)
Temnerature (°C' pH (S.U.)
7.z 9
10.98%
DO (mg/L)
-Vq Conductivity ZCI (Gmhos/cm)
Temperature (0C)
A5. 2 pH(S.U.)
22%
DO (mg/L)
Conductivity 271 (gmhos/cm)
Temperature (CC)
_c52z pH (S.U.)
43.9%
DO (mg/L)
Conductivity zsf (gmhos/cm)
Temperature[°C) 25 Z o
P1R(S.U.)
,9 A 72ff.
DO (mg/L) 7 7 Conductivity 2
(gmhos/cm) 241 Temperature (°C Q 5 3 pH (S.U.)
100%
DO (mg/L)
'71 Conductivity (gmhos/cm)
Temperature (
0 C) pH (SU)
U.)
100% Intake DO (mglL)
Conductivity (Tmhos/cm) 1 Temperatur(C 6:5.f
'(p1 f
(/{
t.410 1
_Z1
.A 0 1
I 4~
dY.9 45.1 -
.t q
I to 17. 5S w KMIM
-Zs.
IS
.\\
A-5 I 8.iZ.
?A I.8
_ B3.1 1z 7. -7S IL I
= I 97 I
0 100 I
75 fl I
740 I &.%
'r l91 1S_ 171 >
jg
~
ZI.s S -j
- zq.e-I ita l F i n a I n.0 Fi n al I 1 Initial I
Final I Initial I
Final I
Initial I
Environmental Testfing Solutions. Inc.
Page 6 of 6 Environmental Testin2 Solutions. Inc.
Page 6 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date:
Il-lc-oS pI Day I
5 I
I" -
Analvst I Concen-Parameter tration I__
CONTROL 5U',
nPH (S.U.)
,
- t DO (mg/Lj
_ _ \\
Conductivity (umhos/cm)
\\,
Temperature (C)
PH (S.U.)
t_._.,
F 10.98%
DO (mp/L)
Conductivity 1
d (gunhos/cm) o Temperature (C)
A pH (S.U.)
I 22%
DO (melL)
D -t _9 Conductivity (umhoslcm)
\\~.
Temperature (0
- S
~
4 nD (S.U.)
43.9%
DO (mg/L)
Conductivity I
tIu(phos/cm)
- 12)
Temperature (°C) pH (S.U.)
oo00 t
72%
DO (mg/L) 97
.9 Conductivity
- 2. 20 (umhos/cm)
Temperature (°C) 2;
- I i
pH (S.U.)
- 7. 'l E1 100%
DO (mglL)
Conductivity Ii@
(umhos/cm)
Temperature (C)
ZS.L L 1 &eio I 7Z-.
11 ?S.I 1_ Initial I
Fina OD (S.U.1 4__.,
100%
Intake DO (mglL)
Conductivity (gmhos/cn)
Initial H Final l
Initial H
a
_ 1a I
inta 92 Final Temperature (°)
\\
W
Environmental Testing Solutions. Inc.
L
.9 5,P1,5 Page Page I of I21_
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0. 10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst lI 4 lb
]
Date analyzed l /I-/(,
IO Iodide reagent: J/IJ,'fl
/f2,01 Acid reagent: $*VA OI 0 1 Z. I Calibration:
I ;;;-X i0.10 mg/L I
1.00 mg/L I Reference standard number lpi % 2s I
s5s?
Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory.control standard:
Reference standard True value (TV)
Measured value (MV)
% RS - MV / TV x 100 number _
(mgfL)
(mglL)
(acceptable range - 90 to 110%) j 0.50
° 03/
/
- .Z '/
Duplicate sample precision:
Sample Sample ID 1 Sample characteristics Residual chlorine
%RPD - U(S - D) /[(S+D)121) a 100 number (m/L)
( acceptable range - i 10%)
Duplicate D o, £o.32Z SampIe measurements*:
Sample Sample I Sample characteristics Residual chlorine number (my/L)
Blank (shouldbe-<0.Lm
- 0__0 3d__/1_
05/us b3 flof
~-.
&16
_________i~
t a.
DSI1.-oz.
/
(10.4Vg J( /
A4
!e
- 11AW, C4 0.40532 Of Iib I's4
- 0. 04 1o, toGIJlol.o6 2Auf =t co io *o60 o3 D61 11
@.Ds a
4z IA 0 at.,
oo60 Nrote: All samples vwere anialyzed in excess of EPA recommended botding time (1I5 minutes) UGiless othenwise noted.
Laboratory contol standard:
Reeirec stndard d True value (TV)
IMeasured value (MV) l
% RS -MV/T IOO j1 Oumber (mglL)
(mg:L)
(acceptable range -490 to 110%
I %ff f
0.50
- 0.
/
1
-~o V..*
04 z --
lwr
- _
z Reviewed by [
Date reviewed I I,-RO.
I
Environmental Testing Solutions, Inc.
7 1b >
Page 6 Y' Page 92= of '2 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter Accumet Model AR25 pH/Ion Meter Analyst [
a I
Date analyzed lI//-/ 16 l.
Iodide reagent I Y,.,1-e
^ -
Acid reagent: I X 417 14 Calibration:
I : Ž14'4Xfi-W 0.10 mW/L I
1.00 mWL I
Reference standard number I
I 2 V5 L
I Note For samples with a residual chlorine of > 1.0 mgfL. the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True v lue (TV)
Measured value (MlV)
% RS -
V / TV x 100 number (mg/L)
(mgtL)
(acceptable range -90 to 110%)
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD - f(S -D) /[(S+D)/211 x 100 number (mr___
(acceptable range -
1 10%)
61le. 05 R440;4
//
i S4o.OO6X4 o511 sIl.o, Duplicate D
Samolc measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mztL)
Blank (should be - < 0. t mg/L) o Note: All Samples Were alyzed in excess of EPA recommended holding time (IS minutes) unless otherwise noted.
L4aboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS-MVITVxIOO number (mgeL)
(mgL)
(acceptable range -n90 to I 10%u) 0.50 0
2-6 z3 0 9 C c i,
Reviewed by I 5t!
Date reviewed I l. I to i
I i
Environmental Testing Solutions. Inc.
r,.6 /.
v/6 Page Page r of
/
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL - 0.10 mg/L Meter Accutnet Model AR25 pH/Ion Meter Iodide reagent rp*%
20 Acid reagent:erA46 Analyst [i IZ Date analyzed [ //_/ f%0 Calibration:
k3777 FEi
- L 0.10 maL I
1.00 mg/L I
Reference standard number 3SS-2
.It75S3Z5 Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV) *
% RS - MV t TV x 100 number *
(mg/L)
(mglL)
(acceptable range - 90 to 110/%)
1~S5 S_
0.50 L
4 j.yo/
Dupliagc samplc precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = ((S - D) /((S+D)/21} x 100 number (mg/L)
(acceptable range-10%)
4 Duplicate 1
D Zo. cS*2.
Sampl measurements.
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
Blank (shouldbe-<0.lOmg/L)
S
.F 0 45o 32 Note: All samples were analyzed in excess of EpA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
j Reference standard True value (TV)
Measured value (MV)
%RS-MV/TV x 100 number (mgrL)
(mgL)
(acceptable range - 90 to 10%) 3 15 3?-
0.50
_2.
_(
I Reviewed by[PE-aZ Date reviewed Ii 1(
50.0 'h Environmental Testing Solutions, Inc.
Page p.
Page I of I
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL - 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Jt I
Date analyzed It - ZO -Soi Iodide reagent j el0 I 2Z Acid reagent: R
-NO o I
Calibration:
7mg/L I
1.00 mg/L Reference standard number XIT SS 3 2.5 XOS 3 Z5 Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV) -
% RS - MV / TV x 100 number (mg/L)
(m-gL)
(acceptable range-90 to 110%/)
xu5s IS-0.50 OC.CMI a.2/
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD - ((S - D) /I(S+D)1211 x 100 number pip (mg/L j
(acceptable range -*10%)
.nuIsQo P
no.cor (to S
.0000 76(1 4
Diplicate
___0._OO_____
II Sample measurements:
Sample Sample ED Sample characteristics Residual chlorine number (metL)
Blank (should be 0.I0 mg/)
W bT 9
W 0.000503 OSIVA. 1Z TVA9-IO -
lOw no coloCr ECcL(r (3.00Mo.5 6,szl A
1 VA-SG 1T fl colCar c-ItAr
<O.GOOoq71 Note: Allsamples wereanalyzed in excess of EPA rcommended holding time (ISminutes) unlessothervise noted.
Laboraloyy control standard:-
Reference standard True value (TV)
IMeasured value (NM)
% RS -MVI/TV I100 number (mgfL)
(mgfL)
(acceptable range -90 to I1010%)
- 5. 5 0.f q. If los
_/06.
Reviewed by K
Date reviewed I I I2.)J05
k.
IL<
.4-..'I I'LI ii..
Environmental Testing Solttions. Inc.
Page ta Page 2Lof 2i_
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst l 7,
Date analyzed Titrate samples to pH = 4.50 S.U.
Thtrant normality and multiplier determination:
Normality Normality (N) of HaSO, pH Factor or Multiplier -
Delonized Titran k
Begin End Total
= (5 ml NaICO, x 0.05)1E
- (Ni 50000)/ 100 ml sample water reference standard
-ml_
ml 0.25/E
- N s 500
- 4... number number
- -(ncwn2bl~erange - 0.018 - 0.022)
Laboratory control standard:_
Reference True value Sample Alkalinity (?V)
% RS - MV I TV tO0 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L)
(acceptable nnge (mg CaCO,IL)
(ml) ml ml ml
-C90ato tab0ic )
JT__lei 1100 00 q.Cf 193 O9 k.L 101 10°f Duplicate saml precision:
Sample Alkalinity
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCOAtL)
((S - D) /I(S+Dy211 x 100.
number (ml) ml ml ml (acceptable range - t 10%)
Duplicate DS Matrix spike recovery:
P a p -
P Reference standard number Spike value (Sv)
I (mg CaCO,/L)
Sample volume (ml)
Begin End ml I ml I To ultiptler Spike alkalinity (A)
(mg CaCO,/L)
I I
I l
Sample alIkalinity (B) lMeasured spike value (M)
/4V/ISV x 100 l
(mg CaCO3/L)
MV = A - B (accep range (mg CaCO/L.)
-75 to I2%
(
Sample measurements:
Sample Volume Begin End Total AlkalInity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOIL) 106h 5at oo 30zco
(.3 1A.4 IZO I_____
_2_
1 T4 1
120 I
Ij.
I.;
Ito11 -
Reviewed by:
De r)v e Date reviewed: 1 14
-]
F, IIp I
I I
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4 II Environmental Testing Solutions* Inc.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3!L Analyst
_I 1
11 Date analyzed Lj -ZO.b-j Titrate samples to pH 4.50 S.U.
Titrant normality and multiplier determination:
Page Page IJ of IIrR.C
,J E
. dIS3 T'-j r e:
.34 9.
pH of Normality Normality (N) of H.SO4 pH Factor or,Multiplier Deionized Titrant check Begin End Total (5 ml Na2CO3 x 0.05)/E
- (.x 50000) 100 ml sample water reference standard ml ml ml 0.251E
= N x 500
- 4.5 S.U.
number number
(_E)
(acceptable range -0.0IS - 0.023) 5.2 J
12A
.-1 O oI_
1
_9.
.0
-C-Ia0.1 0
iqn Lalo~rao
~Veontro standard:
Reference True value Sample Alkalinity (MV)
% RS - MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO0IL)
(acceptable range
.(mg CsCO/L)
(ml) ml ml ml
- 90 to 110%)
IA~lBS
. 100 l1 2q
- 01.
06l Jllo 10..
Du licate saz ple precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Miultiplier (mg CaCOVJL)
((S - D) /[(S+D)/211 x 100 number_
(ml) ml ml ml (acceptable range -
t 10%)
01 0S S6 0
_9 S
- h.
2 Duplicate
- 00) 2,s 3.q q
D D
.~~
Matr spike recoey J Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multplier (mg CaCOj/L) j j(mg CaCOV/L)_
(ml) l ml m1l ml J/ AI#ee 6-5D
/
Q(#
ol>.l 4.lo b,l R.8 Sample alkalinity (B)
M ~easured spike value (INMV
% R -MvV / SV x I100 (mg CaCOIL) l VIV - A - B (acceptable rang (mg CaCOjL) 75 to 125%)
Sample measu'rements:
Sample volume Begin End Total Alkalinity Sample number Sample ID l
(ml) ml ml ml Multiplier (me CaCO/L) 1111 n-o&A
- m. c 42-0 3qS 3A 1.e o.1l qe l 11.13-5 1o lW } IS zo
- 0.
lL..
lo -
_ l l
1 15u'@clx l
I 2a31% 4 2o.1 t 1
1 O5 M U.oi in
.z&2 2.A.j-05118tto~l 1;
n-1 t
19-551 2
l OStt q~i3 2~
6 41 1.1 11 1g 10,11.51 t 2 1 4,5,3 1.l qq 12 C,6111,5.03 j..
6.0
_Aj qL
_1 b s lll15tI Fb WiAthI18.1 I a Is.
Reviewed by:
iDat rd v.1 Date reviewed: I 11-24.as -1
i I
UI 4.
SS 0
a Page 6 v Paue "2 nf I _
Environmental Testing Solutions. Inc.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst t
7 Date analyzed Titrate samples to pH = 4.50 S.U.
Titrant no mali and muldlier determination:
iTNormality Normality (,N) of H 50, pH Factor or ?vtuitiplier Deionkzed Titrant
-chc.
Begin End Total (i ml Na2CO3 x O.OS)/E Ivx 50000y too ml sample water reeec tndard
_{
ml_ _0
.25/E
- j 0
-4.S S.U.
number number
=
=
(E) 4
.0)6 Lcontrol standard:
Reference True value Sample Alkalinity (MNV) % RS -:NV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOIL)
(acceptable range (mg CaCO-)L)
(ml) ml ml ml
-90w to110%)
IMR103 100 100 2 l.T e).
C1.
9.9 Duplicate san ple precision:
Sample Alkalinity
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCOVL)
U(S
-DJ /I(S+D)2l1) x 100 number (ml) ml ml ml (acceptable range t 10%)
bS 1 Ir).
21 10 Z
r 1.3 1.1.
C1.
S 127 Duplicate too 0.0 o
it-to qIi D
1° I_
MfatrLr spike recovery:
Reference Spike value Sample l Spike alkalinity (A) standard number (SV)
J volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCOVL)
(ml) mll l
l Sample alklilcty (B)
Measured spike value (MV)
% R MV / SV s 100 l
(mg CaCO 2L1 l
MV-A-B (acceptable range I(m CaCOVIL)
-75 to 125%)
- ~ ~
~~~
1 O
I I
o
.I Sample measurements:
Sample volume Begin End Total Alkallnity Sample number Sample ID (ml) ml ml ml Miultiplier (mg CaCOV;L) l 5111l4.O lFi~j 3
8I 3 3906 l0-5(oz.e I
0osiI
.o I z I I
I4.1 waZ ce_
(O1l5.5-O5 I_
I j.e I z
I.'
14 I
c 2
2 __
DtSliz.c 1
50 6
20.4 2@2
)l" O5III5. 02.
14WAAO 5 Reviewed by:
I jj g
l 50 3J4 3qp. 4.1a Lc)
Date reviewed:
q.s
[ it-zq'os ]
) 570
Page
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of I
ironmental Testing Sofiftions, Inc.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L rev Aim '. IOO4 T1r~
LCt d
to l
Analyst I li)zt-f I
Date analyzed I I-1t-3 0S I
Titrant normality and multiplier determination:
I.
I..
I I
I II I
I Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
- 0.21E
- (N x 500001 SO ml sample number number (E)
(acceptable range - 0.01I. 0.022)
-Ns 1000 WA115 0JRl'2 0.0 q1q q.q 0.0 zo;_.
2O.z Laboratory control standard:
Reference True value Sample Hardness (MV)
% RS -MV / TV s 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO2/L)
(acceptable range (rng CaCO,/L)
(mI) ml ml ml
- 90 to Ito%)
lA 5S )5 40 5
. 9 W2o 1.
2o.Z e2I2t lo2.
Duplicate sam precision:
Sample Haftness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO0tL)
((S - D) /[(S+Dy21) s 100 number (ml) ml ml ml (acceptiable range e
10%)
IN.Te 45 ft fS 14.to 1I.6 2,.3 4.s Zo S oil Duplicate 2
21.3 Z 5.1.
L e
5 /
m a
kfatrix spike recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Beginl End Total Multiplier (mg CaCOAL)
(mg CaCO3IL) ml) ml -ml ml Sample hardness (B)
Measured spike value (lMlV)
% R -
MV J SV x 100 (mg CaCO3lL) lIV
- A - B (acceptable range (mg CaCO/)
75 to 125%)
145 5I Sam le measurenzents:
Sample volume Begin End Total Hardness Sample number i Sample ID (mD) ml ml ml Multiplier (mg CaCOL)
Blank l _______l_(shouldbe-OmgCaCOI1) l
_l 0.0 0l 0 D l
ZO.z2 NJ) l 1-0843SA ml s RZt 12.o loe 4.e I I&tzoC IsII nn 132 LI 9.71 1
I I
_-_e_
_s I S
uLjI IIz 31.o 7Z.6 4'-0 I ; %1 teo1 O w A s4i I
_ I
_o.
z i
I
- 5.
S 1
-.9 2.6 6
c7511
't.3 MI< oj A
J
__(*
_93_
IQ 51 168-oH Jl-k-Yu g I
f 12.,5 111.31 Y 3
it)
I {qO Note: if >I 5ml or titrant is used. sample must bc diluted.
by:
Reviewed I A3 l
I Date reviewed l
I J
- °5 I
l l
Inc.
Page 5?
Page I
of 2.
4 Matrix:
Analyst l aeanalyzed I Pz O.z,Q
.,nrdrmiftV and multiplier determination:
Total Hardness (EPA Method 130.2)
Water, MDL = I 0 mg CaCO3/L lrfJSf sM'cdtL I 10z t itefereace I I number I
- rmality cuecK standard number
_ Begin End Total ml ml ml I
(E)
I Normality (N) of EDTA
- 0.21E (acceptable range - 0.018 - 0.022) pH Factor or Mjultiplier
- (N x 50000Y 50 ml sample I __
51Vx 1000 B__I I
)fA'IZS I
.o I 0-oUI 10.0I
.1)2OZ I
120 LaboratorY control standard:
I,
4.
K Reference True value Sample Hardness (MV)
% RS - MIV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L)
(acceptable range (mg CaCO,/L)
(ml) ml ml ml
- 90 to 110%)
(40 S
0 10
(
'2.z.
20 L4 I IQ 9.
Duplicate sa le precision:
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (meg CaCO,/L)
((S - D) I((S+Dy2fl x 100 number (ml) ml ml ml (acceptable range - r 10%)
112-to-SSLLL IiLO i
2Z 20 S
L Duplicate De
- 2. 2 0
)A la
.x
.2
-Z Mlatrix spike reco ver:
Reference Spike value Sample I"Spike hardness (A) standard number (SV) volume Begin End Total Mfultiplier
((mg CaCO3/L)
(mg C*CO,/L)
(ml) ml ml j ml I
i s I'
'- i) bSb 114e6 P.* I
' j-
- 63 Sample hardness (B)
Measured spike value (MV)
% R - NIV I SV x I 00 (ng CaCO1IL)
MV - A - B (acceptable range S
(mg CaCOi/L)
- 75 to 125%)
'lq 9-Sample measureinents:
Sample volume Begin End Total hardness Sample Dumber Sample ID (ml) ml ml nml Multiplier (mg CaCO.A
(
bshould CSCO/)
b e -
mAg l3 020 o
0 M
14 1
m S 4e I--
4.Lo lflLQ
. m4S 1-n UZ31 l
'6
_1l 9
_1_It(,_
OJU 2__
28.-53I.(
3.1 C,2-Xill-l
§
§ lep 138.3 3.
10 bts IIS.Q3 Ca-I
___ 3 4rS 3.a O5 11B.tl5 J
2 11* LASO YS l
l__
01s I 16 U
1i j
1
_ 1
_1
_I b1s1 113.b 1 a Ia 1
3s I
1 1 I
Note: If >15ml of titrant is used. sample must be diluted.
by:
Reviewed I
A l
Date reviewed I I Il 2zq os
onmental Testing Solutions, Inc.
Page WQ.
Page 2-of __2 Total Hardness (EPA Method 130.2)
Matrix: Water, MDL 1.0 mg CaCO3IL
(
-Analyst Date analyzed
.. ^.,
ilrant normality 11t I 1'290.5 and multiolier determination:
I -I
. I T
It Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml -
sl
- Ot/E (N x 50000)1 50 ml sample number number
=
(E)
(acceptate C
A r4 1000 Laboratory control standard:
Reference True value Sample Hardness (MV)
% RS = MV /TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOi/L)
(acceptable range (mig CCOill)
(ml) ml nml ml I90 to I1%
\\i(,04050 I)S IZ5' 2.0 2C; qto-O:
'o Duplicate sa moeprecision:
Sample Hardness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL)
J(S - D) /I(S+DY211 z 100 number (ml) ml ml ml (acceptable range -
- 10%).
Ml1Q.O I DAL2di 3
z 1 2.I IB.Z 5.+
20 s
'Duplicate
)
J161, Z35 z3 35 7
z D
I C I
atrirs ike recovery:
Reference Spikevalue Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOIL)
(mg CaCOL)
(ml) ml ml ml I \\6509 qO 53 l,z f-2uSI -. 3 l Z 0 l
Sample hardness (B)
Measured spike value (MV)
% R NV I SV s 1100 (mg CaCO3IL) j MV - A - B (acceptable range I
(mg CaCO)IL) 75 to 125%)
Sample zeasurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO,/L)
Blankc
l l
I~sqould be -
mg CaCO3/L)
I osI1 l 1#i;Q p 10' ll I
,4 Zq.13.(O 2O q2 05"'11 3-4 0
Z.
- 32.
J 9151l.S O5Sll9lj
%11-S331 3}1 1 i
-4, 51150 US
~
-n Os TV lL
?X (Ob13E I
_ I I2 1
1 I 1
1 OriI g ( d 5
(O3 a
I 5 It 11 z..
I '33 z
I sILCI.1 3
I4_
I I
I I
II Note: If >I Smi of titrant is used, sample must be diluted.
by:
Reviewed I
A l
Date reviewed I iit-244 I
Sequoyah Nuclear Plant Biomonitoring November 16-23, 2005 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc.
Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 0.8 i 0.7 0.6 0.5 1.2 s
1.0 t
0.8 0.6 g4 0.4 USEPA Control Limits (+ 2 Standard Deviations)
{JSEPA Warning and Control Limits (7 5th and 9(e Percentile CVs)
=
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I 0.9 0.8 I 0.7 0.6 0.5 INA Laboratory Warning and Control Limits (i1/h and 25th Percentile CVs)
.f...........
a a a a
a a
a a
a a
a I
I I
a 2
1 a
a u.1t Test date 7-day IC25 =25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25
- SA10 or S Control Limits (mean IC25
Environmental Testing Solutions, Inc.
Pimephatespromdes Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water Tat umiber Test date 7-dy yIC CT (dL ]C4 (OL KCI State wd USTIA Labox texy Laheratety USEPA S
Craealndt Sj, Waning nUndis SASS C.1tI UFnIta 5A35 waning Unfts Cr-29 CT+29 cr -9,, CT- + SS,,
C T
+S C
S CS SAMs CT +SAMs USEPA SAVI Centrollimit CT - SM cr + SAM.
CV 1
05-10-05 0.58 2
05-17-05 0.50 0.54 0.05 0.43 0.65 0.07 0.48 0.61 0.11 0.43 0.66 0.21 0.34 0.75 0.24 0.30 0.79 0.10 3
06-07-05 0.62 037 0.06 0.45 0.68 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35 0.78 0.26 0.31 0.82 0.10 4
06-2145 0.61 0.58 0.05 0.47 0.63 0.07 0.31 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.09 5
07-12405 0.53 0.57 0.05 0.47 0.67 0.07 0.50 0.64 0.12 045 0.69 0.22 035 0.78 0.26 031 0.82 0.09 6
07-19-05 0.62 0.58 0.05 048 0.68 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 032 0.84 0.09 7
07-26-05 0.66 0.59 0.05 0.48 0.70 0.07 0.52 0.66 0.12 047 0.71 0.22 0.37 0.81 0.27 032 0.85 0.09 a
0O-0945 0.61 0.59 0.05 0.49 0.69 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.37 0.82 0.27 0.33 0.86 0.09 9
08-16-05 0.32 0.58 0.05 0.48 0.69 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.81 0.26 032 0.85 0.09 10 08-24405 0.63 0.59 0.05 0.48 0.69 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 032 0.85 0.09 11 08-27-05 0.64 0.59 0.05 0.49 0.70 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.2 0.27 033 0.86 0.09 12 09-20-05 0.58 0.59 0.05 0.49 0.69 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.08 13 10405 0.67 0.60 0.05 0.49 0.70 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.83 0.27 033 0.87 0.09 14 10-1105 0.63 0.60 0.05 0.50 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 15 10-18-05 0.55 0.60 0.05 0.50 0.70 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 033 0.87 0.09 16 10-19405 0.59 0.60 0.05 0.50 0.70 0.07 0.53 0.67 13 0.47 0.72 0.23 0.37 0.82 0.27 033 Q87 0.08 17 11-01-05 0.60 0.60 0.05 0.50 0.69 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 033 0.87 0.08 18 11-02-05 0.61 0.60 0.05 0.50 0.0 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 033 0.87 0.08 19 11-01-5 0.60 0.60 0.05 0.51 0.69 0.07 0.53 0.67 0.13 047 0.72 0.23 0.37 0.82 0.27 0.33 0.87 0.08 20 11-16405 0.70 0.60 0.05 0.50 0.70 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.08 NoW 7-d ICg day 25% ikhbkin oncenbation. An esthuation ofthe ancenhatin of pomium chbride hat would came a 25%reduct in Pinephalm pow1h for the teat populatin.
cr - caltl tendency (mean IQ)
S - Standard devition of the M. values.
Laberatey Central and Warning Lbhlts Labo yea l and wwaglhni wre estdbied migdthe tndard dev of he lC whes cupondigto the 10th nd 25th peadile CVa. These mnges are emo tigent th the entoldw ingits eecouended by USEPA for the test method and eidpoitt.
.1= Standard detk eonspodgt the 10 peti CV. (S.
= Q0.12)
SAjS - Standnd deiatim caepondalog to the 25' p entie CV. (SAm - 0.21)
USEPA Contro and Waning Lbnts SAI. - Stsadwd devition conepoodiigto the 75" percentile CV. (SL:7, - 0.38) 8.A,-
Stadad devition wmpondkgto the 90d peentile CV. (SA - 045)
CV - Coefficient of vacithio, of the w akbes.
UeA 200 0hl aDU ai Ae g oe Ed Vdhbiy hiboh 1bxtcdySW IM lhdeflie Nallbtmnt Dan A
c e
3A
-10.
U Ad A
A d O.
Or haw a el aefd fr sAq d1.1 ui,-.1-11-1645
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Pimephales promelas Potassium Chloride Chronic Reference Toxicant Data using Moderately Hard Synthetic Water Test number Test date Control Survival Control Mean Growth CT Cv CT MSD PMSD CT (Y)
(mg/lrvae) for Control OGrowth (I/- (Ig/lr-)
(mg/arva)
(y) for Control crowthCV(%)
(YO) for PMSD (Y.)
1 05-10-05 2
05-17-05 3
06-07-05 4
06-21-05 5
07-12-05 6
07-19-05 7
07-26-05 8
08-09-05 9
08-16-05 10 08-24-05 11 08-27-05 12 09-20-05 13 10-04-05 14 10-11-05 15 10-18-05 16 10-19-05 17 11-01-05 18 11-02-05 19 11-08-05 20 11-16-05 100 100 100 97.5 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 0.772 0.858 0.848 0.622 0.765 0.596 0.807 0.622 0.599 0.802 0.678 0.814 0.785 0.555 0.741 0.556 0.694 0.852 0.754 0.648 0.8 15 0.826 0.775 0.773 0.743 0.753 0.736 0.721 0.729 0.724 0.732 0.736 0.723 0.724 0.714 0.713 0.720 0.722 0.718 10.7 6.0 3.3 7.2.
5.6 2.9-11.5 5.5 12.7 14.9 4.0 8.4 4.5 4.1.
4.9 5.9 1.2 5.5 12.3 13.5 0.10 8.4 0.06 6.7 0.09 6.8 0.09 6.6 0.10 5.9 0.06 6.7 0.11 6.6 0.11 7.3 0.11 8.0 0.14 7.7 0.11 7.7 0.11 7.5 0.06 7.2 0.11 7.1 0.04 7.0 0.08 6.7 0.09 6.6 0.12 6.9 0.15 7.2 0.14 13.5 7.4 10.1 14.5 13.4 9.7 13.2 17.0 18.9 17.4 16.5 13.3 7.3 19.4 5.9 14.6 12.4 14.5 20.0 21.4 10.5 10.4 11.4 11.8 11.4 11.7 12.3 13.1 13.5 13.8 13.7 13.2 13.7 13.2 13.3 13.2 13.3 13.6 14.0 Note.o CV.- Coefficient of variation for control growth On average, the CV for control growth is 7.2% in Environmental Testing Solutions, Tic. PiMephales chronic toxicity tests.
Lower CV bound deterrined by USEPA (10k percentile) - 3.5%.
Upper CV bound determined by USEPA (90' percentile) - 20%
MSD - Minimum SigniWcnt Differmce PMSD - Percent Minimum Signifcaint Difference PMSD is a measure of test precision The PMSD is lie minimum percent difference between the control and treatment that can be declared statistically signiicrant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephalas growth by 14.0% from the control Lower PMSD bound detenmined by USEPA (10' percentile) - 9.4%.
Upper PMSD bound determined by USEPA (90' percentile) - 35%.
CT - Central Tendancy (mean Control Growth, CV, or PMSD)
The lower and upper bounds were calculated by thie USEPA using 205 tests conducted fiom 19 lboratories for PimepAhales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-833-R-00-03. US Envirornmental Protection Agency, Cincinnati. OIL Orgtantnms obtaizedfrom Aquatic RBoSystems, Inc.
I 1-16-05
Environmental Testing Solutions, Inc.
Pimephalespr nelas Control Growth, Coefficient of Variation, and PMSD in Pota ium Chloride Chronic Reference Toxicant Tests 1.25 1.00
.M 0.75 o.so -
025 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
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30 Kentucky Acceptance Limit (< 30.0%)
cl
.C0 20 I,
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40 30 USEPA Upper PMSD Bound (90' percentile < 35.0%)
T d t.....................................e............
20 y.
...................._.A...
- 10 o,
Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Growth, CV, or PMSD)
Control Limits (mean Control Growth, CV, or PMSD 1 2 Standard Deviations)
Environmental Testine Solutions. Inc.
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Environmental Testin2 Solutions. Inc.
Page I of S.
Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Nwnber.
UaLl Dilution preparation Information:
Comments:
KCI CHM number:
(NMH IV9
- 4. -
CSr T
V oS Stock preparation:
50 g Ki/L: Dissolve 50 g MC in l-L Deionized wata S0La -LW L tt:s Dilution prep (mgtL) 300 450 600 750 900 Stock volume (mL) 6 9
12 15 18 Dilnt volume(mL) 994 991 988 985 982 Total vome (L) 1000 1000 1000 1000 1000 Test organism information:
Test information:
l Organism age:
roi4.Is y Randomizing template:
Date and times organisms V\\-SO-O5 T
3 ISo Incubator number and were born between:
shelf location:
Organism source:
Ws AF=k Po iviS oS Artemia lot number o
Transfer bowl information:
pH =
SU Tcmperature =
C Total drying time:
.9 WAS 8-.O 2qt.2_
Date / Time in: l z a- '. 13S0 Average transfer volume:
Date / Time out: It.4.<
-Iy(o
. 0 Oven temperature: _ _'vC.
Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, MRS Analyst feeding feeding time renewal, or batch used time termination time 0
IS0...D wc NIt.,
Ih 11-6S A VoL 15 0
-o IVo ~hIl l-S 6 ii n- *o5 C0oO V~c10 133C0 1\\-%\\-o$s 6 2
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6..L.
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6 Xs-s.... e-*1\\..-- tl
.I
- 1 Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
1 s 20%
7-day LC50 lo.._
Average weight per initial larvae:
t 5. b
~%8 I.
NOEC I
£ Average weight per surviving larvae:
0 0-64.
2 0.25 mgftmae LOEC
-5 O I ChV 110. t IC25 (o0l.
E '. Tee S
.sS sn Environmental Testing Solutions, Inc.
Page 2 ofS 5 Species: Pimephalespromelas PpKCICR Test Number: __4 Survival and Growth Data Day Control l
300 mg KCIIL 450 mg KCIL A
B C
D E
I F G
H I
J K
L 2-0 10 I lO 10 0
/0//0 10
/0
/0
/0 1
10
/0 10 /0 10 10 1I 10 C
10 10
'0 3
4_____
to~i I t 10
/0 Ia 10 10O
/0
/O 10
/0 5 Qo /0 to
/0 0o
_0 I
/o lo l
/0 6
10 JO 110 10 1a
/0 10
/0
/0
/0 I10
£0 7
sL6-I'.fr to l
1 o
110 to 45
'O 0 lo_
10 10 ld A -
n weight lq 'L.6 IG.IZ z40o7 1 5. 87 Ij
!Z I5.it Tray color code::..z1.
5.7" 1
iIA Analyst:
16l3 I 9 l 6.1.
1 76 1q¶
- 6. 31 B - Pan + Larvae weight Analyst:
- 5. I, 20.1 2%
=t 2ZA.I x4 22 -O AS° Larvae weight (mg) - A - B Sq0,1lc1Rt
.12) b.2bo S,30 11 Sr44-3 1.S2 16.YS 5-gil 9
Weight per Initial number of larvae (mg)
- C I Initial number of larvae 0IO
^.. I,?
V.
IV
'at!
t~.
o N
o~.
o.
4, 0*
P i
I Average Percent weight per reduction Initial from control O. 6qR number of (1/%)
larvae I _ _ _
o.o&0
-4=
o.-106
[ e.A t, Comment codes: c - clear, d - dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
I Environmiental Testing Solutios Inc Specles: Pimephalespromelas PpKCICR Test Numb Pa'g er:
r Survival and Growth Data r
Day 600 mg KCIIL 750 mg KCUL 900 mg
-
-
w -
I -
W -
M N
0 P
Q R
S I T U
V IC) I 0
to 10 lo l-o I-1l
/O I0 c
c_ qtA I 0c 10 02
,t\\ -73 2
I1D 4
101A Q
101 0
i-e 3
q id O
lo 1 id 54 75 v
_ 0 q
9id io-q
_d 5
tT oq q
q' 4 _7
{,i-q' lO n
6 7
-d A - Pan weight (m
- 16.
1 Tray tolor code::
Analyst J
1156 B - Pan + Larvae wetght (mg)r Z21.2 U. ZI I1B.8 9 20 A614-U 1-3.14 11.9 N-2o~J )
ItA l.
Larvae weight (mg) - A - B Ie-M ia
.t n'A 3,9%L q. ja
.3 4.-67 1j.50 1.qwo 0. bb 1.,Ib 3.#4 Weight per Initial number of larvae (mg) i
'S C I Inltdal nulmber of larvae 01
.a C K
JX t
- 4V 9O O
\\
Oo c
r o
Average Percent weight per reduction Initial from control a
Z.Z!-laz
- o. 41 3a4. 17c o.iLs
-,L.27 number f
(%)
larvae (mg)
I..
I Comment codes: c - clear, d - dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed: 4 Comments:
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pmephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test _m:naw PpKCICR d U (134 at 351 Depot St.)
Test dates:
No"Vener 16-23,2005 PRvdwed CmharmgwL tidw.a-,F 51_.bwe A-Pa.dsib *.e a-
+La.t L-.rebdhi WdsloJ keebe as-
.ew Cuedd.
ladiina Wuigbaleai S.:
ft..
,sl IN _edeata unm KC1k as kov..
- .A.3
_m SeAmhsee ob-iOPw odhm(.
('A) bwhe Ml "N
M A
10 10 14.68 20.06 5.40 0.540 0.540 Conto C
10 10 16.13 23.62 7.49 0.749 0.648 13.5.
0.749 100.0 0.648 135 Not apPileable C
30 10 16.12 22.35 6.73 0.673063 D
10 10 14.99 21.29 6.30 0.630 0.630 D
10 8
15.07 20.37 5.30 0.663 0.530 10 10 16.16 23.38 7.72 0.772 0.713 7.4 0.6750
."11 C
3 10 lO 15.37 22.62 6.75 0.675 0675 H
to 30 16.26 23.69 7.43 0.743 0.743 I
10 10 14.39 22A41 7.52 0.752 0.752 450 K
t 10 14.29 21.04
.75 0.675 706
.4 0.675 10, o.7i 12A AO KC 10 10 16.31 22.30 S."9 0.599 0.599 L
O 10 15.9" 23.95 7.97 0.797 0.797 M
to 10 14.59 21.2t 6.69 0.669 0.669 600 N
10 9
15.99 21.92 5.93 0.659 0.717 t9 0.573 92.5
.662 7.5
-2.2 o
10 9
14.0 21.21 7.12 0.791 0.712 Or o
9 14.73 21.52 6.74 0.749 0.674 O
10 5
16.20 20.12 3.92 0.7U4 0.392 750 R
10 6
14.56 '
It.9 433 0.722 0.717 8.1 0.432 60.0 0.27 5J 341 S
10 6
H4.6 13.97 4.32 0.720 O3 T
t0 7
16.16 20.66 4.50 0.643 0.450 T
10 3
16.14 S
17.60 1.46 0.437 0.146 900 V
10 14.82 15.48 0.66 0.660 0.54 25.7 0.066 30.0 6.163 72.2 74.2 W
10 3
15.06 16.22 1.16 0.337 0.116 X
to 5
34.63-13.05 3.42 0.634
_ _ _ _ _ _ _03 2_
Dusett's MSD vae":
PMSD:
0.1337 21A4 MSD -
Mionium Signifcat Dforwnc PMSD -
Pon Min Significant Difftrence PMSD isa weastur oftest peision. The PMSD is the miniuaun percent diffeence between the conleel and threat t-at cn be decled statistlcauly a;!iDicict Is a whle eflien toxicity test. On aveage. a *ipuleant difference oeam foe Environmental Tes 5 0 Solutiona. Ic. chronic toxicity testa when a toxicant reduces PRwqhafer roth by 14.0% ftos lt control (deleunined tfogh refteence boxicant testin)
Lower PMSD bound detesned by USEPA (ltb Percentile) - 9.4%.
Upper PMSD bound deteimed by USEPA (Stb pentie) - 35%.
The lower ad upper bo s were caclaed by die USEPA sing 205 tea conducted front19 labatories for Pinepheles growth i chronic eftnce toxicant tests.
USEPA. 2000 Understanding and Accounting r Method Vriabilty in Whole Efiluent Toi* Ap N s Under th National Polltante Di-me Eknimetion roramn EPA433-R.0003. US Enybrsnsncnta Protection Agency.
elsc t OIL 11-1605 Orgonnsno obaotnedi-ln Aquatic Rfo~ysleu.
I-c
Environmental Testing Solutions, Inc.
Statisdcal Analyses LA11W Flab Crowth and Survive Tast-7 Day Growth S2utDetc:
11/16/205 Test ID:
PpKCER Sapl>
M.
REP-RetToxiart End Ddc.
I1/2Mar=05 Lab aD El6.E c lutg Sol Sampk Type:
Ml-FPotkm ddoride Sample Date:
potoot FWCHR-BPA421R-C2.013 Tet Speies:
PP-P&Mpbake, poah Comnments Ceu 1
2 3
4 ICtrol 0.5400 0.7490 0.6730 0.6300 30M 0.5300 0.7720 0.6750 0.7430 450 0.7520 0.6750 0.5990 07970 600 0.6690 0.5930 0.7120 0.6740 750 03920 0.4330 0.4320 04500 900 0.1460 0.0660 0.1160 0.3420 Tnr utcow.
Uhlramntmed 1.Taled 1l.onk CeescmglL Mum N-Mu' mu' Mil Mal CV%
N t.Stat Cical MSD mu' N-MU' D.Cnoml 06410 I.OOD 0.64S0 054CO 7
0490 13.A51 4
Q.6779 1.000 300
.6100 1.0494 0.6tO 0.5300 0.7720 12.175 4
4Q521 2290 0.137 0.6779 1.0000 450 0.705 1.0091 0.7051 "5990 Q7970 12.354 4
4.954 2.20 0.137 0Q6779 1.00 600 0.662 0.6620
.5930 0.7120 7.530 4
4231 2290 0.137 06620
.9765 750 0.4268 0.6516 0.6261 0.3920 Q45X 5.763 4
0.4261 0.6295 90 0.1675 0.25S5 0.1675 Q0660 0.3420 72.193 4
01675 0.2471 Axeliory TU Stade Otical skew Kart Sbrapko.VWM Tatrldimaesnrmuatdistrttim
> 0.0))
0.943732013 O.1Q44
.053452712 461994917 BanrtS TethdAi eq a "v ne
-0.70) 1444379091 1134416675 iesiTat (1a2L US)
NOEC LOEC CIv TU MSDo MSDp MSu MSK F4Prab d
Dimnetla Tat 600
>600 0.131692114 0214031149 0.002415396 0.007336063 0.7977C9525 3,12 nrab3 vs D41~l Lkesr Interpeotion (00 Ruampls)
Polut nR/L SD s5% Clzp)
Skew 1005 611.A6 94.63 0.00 620.74
-2.9101 ICIO 63.00 34.11 539.15 653.40
.7.5504 ICis 654.69 15.15 591.12 677.05 1.2063 1.0 IC20 67630 13.56 616.70 700.70
.0.8554 OS IC25 697.91 1265 64216 724.51 46090 1040 761.57 10.96 724.92 79045 40510 IC50 100.10 15.61 76531 865.13 03336 0'7 0.1
.0.1 ZO,.e a
2C0 400 600 680 10W Dose mgL D~es.inp' FltL Otantams -
Mohhidf-m Apude BioSystersa, h..1.1.
11-1645
I
.nt 5 Environmental Testing Solutio S nslc.
I Species: Pimephalespromelas PpKCICR Test Number:
I Dal hoFemistrv I
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~Day__
AnalIyst-A K Concentration Parameter
.3 CONTROL pHl (S.U.)
I.-
5 e
2 3
.7 DO (mg/L)
Conductivity all31 1
Alkalinity 6_
e
\\
Hardness e6\\
(mg CagqA/)4a r_
Temperature Zl A5 2,-
-zt
>.S z4 A
-24 A4 pH (S.U.
o 1.91 _
t{
e-
.6 7,77 DO.(mg/L.)
1.5 I 1.6 S.
7.5 300 mg KCI/L Conductivity wa g^
Temnperature ZVA
~ >oA 2l-4 I
.8 Zi5 2
pH (S.U.)
-1.9 0 1.85
.l
.S ec 7.i DO (mg/L)
-I.'S
- 1. z 1
1.7 7 (
- 7. 3 450 mg KCU/L Cpmosducm)i IlZ V
111° 1110O Temperature 2-A zq 24 Zq2t.j 2q., -
zA
-L S.%
_pH (S.U.)
-1.,90 1.12 n. i 9S 30-66 7
DO (mng/L)
S
- n.
4 1.1
,.10 r.Y 600 mg KICCL Conductivity 1 3520 15E 3ao 1
lA~o
.Temperature ZLIA z-~lzc
-c9 zzqt 4ci l pR (S.U.)
.ola
-J. Es
'1AR 7.4?
l3.03 7.7 t DO (mg/L)
I.
- 15.
(a 1
4.(o I. I 750 mg KCi/L Cmonductiit I1SO 1 \\lNO ItOo4 Temjperature ZS. I 7
l Z
e v 2.b Zl-
_pH (S.U.)
1.52 16 l
481 6.03 7.
78 DO (mg/L)
-1.Io l
X 5c
%o X
900 mg KCi/L Conductivity MOO l
0 q
l 3
Temperature Z5. O 2qJ1 Zi Jz
- Lt -8
- 7. -0 STCI Condu ctivity.SIDO 0110 _
L Initial I
Final I Initial -1 Final I Initial I Final I
I
Envirnn-I4A I TUAILLU-A.
c-i-4-c Ine.
Page 5 of 5 I-Species: Pimephaes promelas PpKCICR Test Number: 0r I
may I
3 I
4 I
5 I6
&Af 1
1 I
Concentration Parai Li CONTROL
- ?I HS pH (S.U.)
89.oc DO (mg/L)
-7.9 300 mg KCIIL Conductivity (pmhos/cm)
Temperature
____(°C)__
(0
° CQ 78.00 pHI (S.U.)
8.oa DO (mg/L) 450 mg KCI/L Conductivity (i~mhoslcm)
Temperature pH_(S.U.)
C
.O 600 mg KCUL DO (mglL) 7 -7 Conductivity (lmhos/cm) 1370 Temperature pH II..
9.02.
DO (mg/L) L
'.7 750 mg KCUL Conductivity (Amhos/cm) 14'5'Q Temperature 2t.1 (OC )
pH(S.U.)
Sro2.
DO (mg/L) 1 900 mg KCI/L Conductivity
~~(gimhos/cxn)
I a
Temperature STOCK Conductivity
_________(pmhos/cm)
I vsc z4.1
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N653I I
I t4A I
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I z4-1 I 24 A Ii -
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-i_ii0 zg IzIntia Lit IZS.OI Initial
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Sodium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water I
I I
I I
I
~I I
l 1.12 USEPA Control Limits (I 2 Standard Deviations)
I I
I 1.10 F 1.08 1.06 1.04 V
.I I
I I
~~~
. ~.
I Il 2.5 U
z ve U
N.
2.0 1.5 1.0 0.5 USEPA Warning and Control Limits (7 5 h and 9 0'h Percentile Czs) 1.4 1.3 1.2 1.1 1.0 0.9 nQ Laboratory Warning and Control Limits (J th and 2 5 th Percentile CVs) v.0 Test date 7-day IC2. = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 4-SA.1 or SA.75)
.Control Limits (mean 1C25 +/- S A.251 SA.90 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Tozicant Control Chart for Cerlodaphnla dubia using Moderately Hard Synthetic Water Test number Ted date 7-day IC,,
CT (g/L NaC)
(g/LNaCI)
State anitUSEPA S
C ltd Lbtb SA CT-2S CT4+2S Laboratury WaCn-ig units
Laberatery CenulLith SSS CT - Sals CT + S, USEPA Waing LUnits CT - 8AL CT + SLs USEPA SAM CenSre CSLi CT - 9SL CT + SA.
CV 1
06-0844 1.06 2
07-0744 1.06 3
0-10404 I.06 4
09-14-04 1.05 5
09-14-04 1.07 6
1045-04 1.07 7
11-02-04 I.OC a
12-07-04 1.0O 9
01-11-05 1.06 10 02-03S05 1.05 11 03-01-05 1.0O 12 04-0545 1.09 13 054345 1.07 14 06-07405 1.05 15 07-12.05 1.06 16 07.1245 1.06 17 0-09-4S 1.03 IS 09-13405 1.04 19 10-04-05 1.09 20 11-0145 1.03 1.06 0.00 1.05 1.06 0.08 0.97 1.06 0.00 1.05 1.06 0.08 0.97 1.06 0.00 1.05 1.06 0.08 0.97 1.06 0.01 1.04 1.07 0.08 0.97 1.06 0.01 1.04 1.08 0.08 0.98 1.06 0.01 1.04 1.03 0.03 0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.03 0.03 0.93 1.06 0.01 1.04 1.03 0.03
.0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.07 0.01 1.04 1.09 0.09 0.93 1.07 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.09 0.09 0.9S 1.06 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.06 0.01 1.04 1.09 0.09 0.93 1.07 0.01 1.04 1.09 0.09 0.93 1.14 0.13 L14 0.13 1.14 0.13 1.14 0.18
.LU5 0.13 1.14 0.13 1.15 0.1 1.15 0.13 1.15 0.13 1.15 0.18
.LU5 0.13
.LU5 0.13 1.15 0.18 1.L5 0.18 1.15 0.18 1.15 0.13 1.15 0.13
.LU5 0.18
- LS 0.1S 0.33 0.33 0.38 0.38 0.88 0.88 0.83 0.83 0.83 0.83 0.83 0.83 0.88 0.38 0.38 0.38 0.38 0.88 0.88 1.24 0.43 1.24 OAS 1.24 0.43 1.24 0.48 1.24 0.48 1.24 0.43 1.24 0.4S 1.24 0AS 1.24 0.4S 1.24 0.4S 1.23 0.48 1.25 0.43 1.25 0.48
.2U 0.48 1.24 0.4S 1.2S 0.43 1.24 0.48 1.25 0.43 1.25 0.4S 0.53 0.53 0.53 0.53 0.53 0.53 0.53 0.58 OSS 0.SS OmS 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 0.59 1.53 1.53 1.53 1.53 1.54 1.54 I4 1.54
.1.54 1.54 1.54 1.54 1.54 1.54 1.54
.54 1.54 1.54 1.54 0.66 0.40 0.66 OAO 0.65 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 0.66 0.40 J71 0.00 1.71 0.00 1.71 0.00 J71 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.73 0.01 1.73 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.2 0.01 1.73 0.01 Note:
7-d ICs day 25% iniuibitiooneentmation. An estiution of 6t concenhation of odium codide tht would cause a 25% reduction in Ccriodophnfa itpieduction for tho lost population.
CT - Cential tendency (an XC 2 1).
S - Standaird deviation of tbe ECz values Laboatory Cetirtl and Wanig UnIts Labotorycontolandwmninglint warnestblihedusg3thedrddeviationf theivalucasorepondingtothelOthand25thpercenileCV Theserngesarenmoeingtththecontl andwaning linits nruoi mendcd by USEPA for the t nethad and endpoint SAJi - Standard deviation corresponding to the 10 percentile CV. (SoI - 0.08) 8As - Standent devistion e snpdingto the 23 pereentile CV. (SA2, - 0.17)
USEPA CTntrl and Waning Units Sss - Standard deviation conqesonding to the 75*percntile CV. (SA7 - 0.45)
HA."- Standard deviation comiionding to the 90" pementile CV. (SA - 0.62)
CV - Coefficient of variation of the IC values.
USEPA 2000. Understanding and Accounting forMethd Vadability in Whole Effluent Toxicity Applications Underthe National Pollutant Dichare EliminationPcognum EPA-833-R-00-003. US Bnvimnrental PretectionAgency, Cincinnati, OIL 110145
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Cerodaphnia dubia using Moderately Hard Synthetic Water est TControl Control Mean nunmber Test date Reproduction forC(
(%)
(offsiring/fernale)
Rep (offs CT CV CT MSD PMSD CT for Control (Y.)
Reproduction CV (%/.)
CIA) for PMSD (Y) 1 06-08-04 100 2
07407-04 100 3
08-10-04 100 4
09-14-04 100 5
09-14-04 100 6
10-05-04 100 7
11-02-04 100 8
12-07.04 100 9
01-11-05 100 10 02-08405 100 11 03-01-05 100 12 04.05.05 100 13 05-03405 100 14 06-07-05 100 15 07-12-05 100 16 07-12-05 100 17 08-09-05 100 18 09-13-05 100 19 10-04-05 100 20 11-01-05 100 32.9 33.3 27.4 28.7 28.8 29.6 30.5 31.8 31.0 30.0 29.9 32.4 29.9 30.4 30.4 31.1 28.3 27.9 27.0 28.4 33.1 31.2 30.6 30.2 30.1 30.2 30.4 30.4 30.4 30.4 30.5 30.5 30.5 30.5 30.5 30.4 30.2 30.1 30.0 6.8 6.3 5.8 4.9 7.5 8.2 8.5 4.9 9.4 6.8 6.6 6.9 7.0 5.0 7.5 7.2 7.3 7.3 5.8 10.3 6.6 6.3 6.0 6.3 6.6 6.8 6.6 6.9 6.9 6.9 6.9 6.9 6.7 6.8 6.8 6.8 6.9 6.8 7.0 3.0 9.0 2.6 7.8 2.3 8.6 2.4 8.2 3.0 10.5 2.8 9.6 2.7 8.8 2.5 7.9 3.1 10.0 2.4 8.0 2.9 9.9 3.1 9.6 2.9 9.8 2.6 8.5 2.7 8.8 3.2 10.2 2.9 10.3 3.9 13.9 3.0 11.1 3.8 13.2 8.4 8.4 8.4 8.8 8.9 8.9 8.8 8.9 8.8 8.9 9.0 9.1 9.0 9.0 9.1
-9.2 9.4 9.5 9.7 Note:
CV Coefficientofvariaiion for control Teproduction.
On average, the CV for control reproduction is 7.0% in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity Lower CV bound detemined by USEPA (10 percentile) - 8.9%.
UpVer CV bound deternined byUSEPA (90' percentile) - 42%
MNSD - Mnimu Sigeificant Difference PMSD -
Percent Minimun Significant Difference PMSD Is a measur of testprecision. The PMSD is the niniumn percent difference between the control and trtatrnent that can be declared statistically signifcant in a whole effluent toxicity test On average, a significant diffirence occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Cepiodaphnia reproduction by 9.7% fi-n the controL Lower PMSD bound detenrrinedbyUSEPA(10*'percentle) - 11%.
Upper PMSD bound detenrined by USEPA (90" percentle) - 37%.
CT - Central Tendancy (Mean Control Reproduction, CV, orPMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerlodaphnia reproduction in chronic Isrcnce toxicant tests.
USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Disdae Eirnination Program. EPA-833-RO0-003. US Environ=tal Protection Agency, Cincinnati, OH.
I-J41-05
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 1
B I I
I I
I 1 1
I 325~......35...........
- %2 5 -
o 320 U
15 __
Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I
I I'
I I
I I
IB s 40
_ oNorth Carolina Acceptance Limit (< 40.0%h)
Po 30 c:*
Kentucky Acceptance Limit (< 30.0%)
C 20 c
C 10 42 B
I I
I I
I I
I I
I I
Iv I
I I
I I
40 USEPA Upper PMSD Bound (90d percentile < 37.0%)
30 20 C1; 0
I I
I I
I I
I I I
I I
I I
Id a
Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean Control Reproduction, CV, or PMSD)
Control Limits (mean Control Reproduction, CV, or PMSD E 2 Standard Deviations)
[ Environmental Testing Solutions, Inc.
r I.
Dilution preparation informadon:
NaCI CHM number CAl 12 Stock preparation:
100 g NaC deionized' Dilution prep Img/L) 600 800 Stock volume (mL.)
129 Diluent volume (mL) 1491 148 Total volume (mL) 1500
!5 Test frtanism information:
Organism age:
< 24-hou Date and times organisms II-Ok-a were born between:
Organism source:
I-D s-c Transfer bowl information:
pH =-1, Page I of 6 loride Chronic Reference Toxicant Test A.821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCLCR #: _ Az=
Comments:
Lc t/I (dissolve 50 g NaCI in 500 ml w tcr) 3 001120-1400 I S IS 2 1 148S 1482 1479 IS500 0
t00 IS00 Test information:
irs old Randomizing template:
'/8XU
-S<1tMc too Incubator number and shelf location:
4 Y
2
-F YCT batch:
____0_0 I SU Temperature =s4RC Selenastrum batch:
jIO-;&-06o Daily renewal information:
Day Date Test initiation, MHS water Analyst renewal, or batch used termination time 0
l--O5 j
t
&2405 3 2
02 -
I-_^
I A
X 2 I-b-Os 104 10 -c -l o5'sA t-%k
- 3. wo-O 1 1 I.
10 -Os t5 2
lI;ij
\\ObOCS IO Ib
'i z,
i i
I It
- E e I,m-Os I0on3 ll-oS-oS A LS l-OC-OS Io51 0
Control information:
Acceptance criteria Summary oftestendoints:
%ofMaleAdults:
l 5
S20%
7-dayLC5O
'l N
'O4
% Adults having 3" Broods:
/00l z
8 80%
NOEC Lo_
%Mortality:
l 7
S20%
LOEC two I Mean Offspring/Female:
l
- 22.
1 I 5.0 offspringlfemale ChV i\\is.
4
%CVM to.5Z
{
<40.0%
lIC25 l
l.O I
I Environmental Testing Solutions, Inc.
I Species: Ceriodaphnia dubia I.
CONTROL Page 2 of 6 CdNaCLCR#:
A 2J Survival and Reproduction Data I
I" i.-
i, I.
I I
I.
I Replicate number Day 1
213 4
5 6
B 9 9 10 1
Young produced l L
CD 0
CO Adult mortality Il L
2 Young produced O
0 t0lC
^l^
(
)
C Adult mortality L-4 jul '-
1_
L-I.
l.
3 Young produced O
O 0
0 O
Adult mortality l-I Li t-k- -
4 Young produced 44 -- <_
Adult mortality l
I L
Young produced l
LIZ 1
l C:)
.I l AdultmiortalitY l
- k.
l-L-
l 6
Youngproduced 12... l 0lD 7I0it I
i 0 lAdult mortality
-_L-L-
L-I L-l I '
l l
Lt_
7 Young produced Ij l
0 11 l I
I Zl I 7
l 1J IL 3
13 Total youngproduced 3l 1
.l l6 l.
3 25 To z.
lzq ti 12.1 Final Adult Mortality T
L l
3 t
c 1z Xfor3re Broods
-X I
x Note: Adult mortality (L live,D"dcad) 4 SPUT taaotS Concentraaon:l l%Mortalita:
l l
Mean Offspring/Female:
l
.'A 600 mg NaCllL Survival and Reproduction Data I I Replicate number Day
._l___
2 l
3 4
5 6
717 8 9
110 I
Youngproduced l c) l l
l l
I 0
0 l
COl Adult mortaltyI<
t Qll 2
Yo_1 O~
L.0~L--ung produced C
.)I i
L U3 j-Adult mortality I
L 3
Youngproduced I..li lo i 00 l
o l Ol O l.(O) l Adult mortality \\ l UJ L
l 4
Young produced I Si 9
i 3 1 5 i
Adult mortality U
L S
Youngproduced l1 1l 1 :'V% lI I l IlC/)
I l
0Il0 17 Adult mortality LC t-L L-l l
l 6
Young produced l
lal3 l
0 0 l 0
3 ll mJAdult mortality L-I %,.
=
t1' L-tCVC %.
7j Young produced IL7 I '3 15I
- 12. Z 7ri i- [1515116.
Total young produced I
I I
Final Adult Mortality
[L E
t l
C 1
I I
I:
I II.1.
Note: Adult mortality (L - live, D - dead)
-AsitT 60bob l Concentration:
%Mortality:
M0 I Mean Offspring/Female:
I 29z.
% Reduction from Control:
-2.1%
I Environmental Testing Solutions, Inc.
Page 3 of 6 Species: Ceriod I
800 ma Ni ri
'aphnia dubia CdNaCLCR #:
A42 Survival and Reproduction Data Replicate number Day 213 4
5 6
1 7 8
1 9
10 I
lYoung produced
)l
_O O
O l
O Ol O
C Adult mortality U
L l
U.
2 Young produced O
O C)
Adult mortality
[
l 1
t-l l L
ljU 3
Young produced O
4 Cl lO O
l O Adult mortality kU 1J L
l 4
Young produced 5
i S I -3
'-A tiA L3
-iI Adult mortality t
L l U l
[
S Young produced CZ l4 I
j 10 x2I l
I I
1 Adult mortality U
t 6
Young produced
[
O 0
l-O O
I Adult mortality l
AUJU.
l l V--I__
7 Young produced l
13 I
I I I l
I Total young produced l
o lzj 7I1 29 7.1 74z T
El 2TT1 Final Adult Mortality l
C C
l l
Note: Adult mortality (L live, D - dead)
VW-8 I*lConcenration:
%Mortality:
I Mean Offspring/Female:
I Zi.1
% Reduction from Control:
- 1. 97.
1000 mg NaCI/L Survivd and Reproduction Data I1i Replicate number
___2___3__4_____7 8
I 9 10 y
_ Young produced C)
) J
° Q
KC) 0o I
Adult mortality l
U U
2 Young produced Q
ll Oj O
Adult mortality IL Il L IL 3
Young produced j 0
j 0
j Cj j jJo j
Cj
° Adult mortality LAT7\\
l o
l l
4 Young produced ZS~I L A 1 LA LA LAW -V Adult mortality
-1 VCTIL.
II-U l
l Young produced l
9 l
l 10 3 l
'I l 3 l1 Adult mortality I L-L llL L
Young produced j
AL 0lOT I.L.
0 1el 10 a
l Adult mortality l
4 L...C I.
U
- lC 1CJ f
L 7
Young produced I
/ O l l
1 l,
c7 1 o Totalyoungproduced 1 3 2
iZ3 23 23 21 V.4 I__
F la A
d l
i9 l-ll L-Note: Adult mortality (L - live, D a dead)
Lfrr 6jC op Concentration:
l
% Mortality:
- 07. I l Mean Offspring/Female:
7 2z.1 I
% Reduction from Control:
Il oC6 l
- Envij i..
I a:.
)
I@
I I
ronmental Testing Solutions, Inc.
Species: Ceriodaphnia dubia 1200 mg NaCVL Page 4 of 6 CdNaCLCR #:. ___
Survival and Reproduction Data Replicate number Day-S 6
7 8
9 10 I
Young produced C.
O)
C L)
()To
_ Adult mtortality L
2 Young produced l LI Adult mortality l
_L l-.
L-3 Young produced O
C
)
O O
CD L
O
_O_
Adult mortality_.
- l -
4 Young produced i
Z-rz I
I Adult mortality U I U
I l
L 1
L U
s Young produced I
A
£8jCL Adult mortality l
II
{
It i
I' 6
Young produced l0 LA, O
- O l
l3 CO O
JAdult mortality CZ1.
4 1_..;,I I~
2I L Z-1 7
1 Young produced 1
13 l I
Totalyoung produced lZ2 l2t l-l i
l FinalAdult l ortality I
Note: Adult mo rta lity (L-live, D-tead) lConcentration:I 7 % Reduction Y dom Contol:
pre 5.7 -
1400 m-A NaCI/L Survival and Reproduction Data I
Replicate numberI I
Young produced l z4 l
O O
1 0 1 0
F Adult mortality l
L l
l 2 jYoung produeed j
C)t N A dult m ortali ty (L
li e 0 d d l__._
l I o centration Aduat morta7i ty 4i Young producedOlflOlm2e 0
- 11.
0 lAdult mortality l
'L-l lI
_l t
l s
-Yo un a
I L 3
i Lti5 i
l-I
~~
Reuto fro Cotrl I~ 1 i II16 1 mYo naCI/L O
SurvralIn Reproduction Data Da ________I________L L
L I
2 I1 10 fl 7 tYoung produced l
0 0
Tot3l young produced pl ll 4
Fin rl Adu lt Mortalit y
LL L
Note: A dult mortality (L - live, D d ad)
Concentration:
% Mortality:
l 07.
l Mean Offspring/Female:
l95
% Reduction from Control: l
(,t.. S
Environmental Testing Solutions, Inc.
Verification of Ceriodaphnia Reproduction Totals Control Re plcate number Day 1
2 3
4 5
6 7
8 9 l 10 Tot O
O O
O O
O O
I O 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 4
4 4
3 4
3 4
3 4
37 S
0 12 10 12 10 2
0 11 0
10 67 6
13 0
0 0
0 12 11 0
110 47 7
14 181214 12 12 11 14 13 13 133 Total 31 34 26 30 25 30 25 29 27 27 284 600 mg NaCVL Re lkate number Day 6
1 7 8l 9 l 10 Totsl 1
O O
O I O O 0 0
0 O
O 0
2 0
0 0
0 00 0
0 0
0 0
3 0
0 0
0 O
O 0
0 0
0 0
4 4
5 4
4 4
3 4
4 3
5 40 5
12 11 1
11 1 1 10 10 10 10 12 98 6
0 0
13 0
0 0
0 0
0 0
13 7
12 13 15 12 14 17 12 15 16 141 Total 8
29 33 2?
29 30 26 29 28 33 292 800 mg NaCI/L Day RRe plcate number l
o l
1 2 1 3 1 4 5
6 1 7 8 1 Total 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
4 4
3 3
4 4
4 4
39.
5 12 14 10 11 10 12 9
10 0
1 11 99 6
0 0
0 1 1 0
0 0
10 0
1 1 7
13 1 11 13 1 11 16 14 14 12 14 12 130 Total 29 301 27 _I27 29 29 27 26 2
27 279 1000 mg NaCI/L DayRe Pleate numberToa Day 1
2 3
4 5
6 78 9
10 I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 O
O 0
° O
00 0
4 4
4 3
31 4 3
4 4
4 3
36 5
1 13 0
12 0
9 91 0 10 9
63 6
10 01 10I 0 11 0
01 9 0
0 40 7
17 141 13 I14 14 11I 10I101 12 128 Total 32 3
26 292
-23 r2-3 23 2
24 267 1200 mg NaCl/L.
a
.e
.l.at, number Total a
1 2
3 4
5 6 l 7 8
9 l 10 1
0 0
0 0
0 0
0 0
0 I0 0
2 0
0 0
0 0
0 0
0 O
O 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
4 3
- 1.
2 5
2 2
1 3
27 5
5 5
8 6
S 8
4 0
8 6
55 6
0 4
0 0
0 0
0 3
0 0
7 7
13 11 0
16 0
4 Total 22 24 111 7
13 6
5 5
9 11 1400 mg NaCIIL Day T
2 Replicate number Total 4
256 7
9 1
0 1 7 8
1 0
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
0 2
0 0
3 3
0 0
1 13 5
2 3
7 3
4 4
5 9
4 1 0 41 6
0 0 1 0 1 0 0
0 0
0 1 0_17 7
7 1I 5
9306050 34 Toal 7
8 f144 -12 17
'7 14 9
9 8
9
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species:. Cerodaphnla dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Teat number:
CdN*ClCR #71 (#42 at 351 Depot St.)
Test dates:
November 0140, 200S Revetwed by:
Concentration Replicate number I Survival Average reproduction of r
(m.gL NaCI) 1 2
3 4
5 6
7 a10 (9)
(offsprlnfemale)
I'_ (-t(")
Control 31 34 26 30 25 30 25 29 27 27 100 28.4 103 Not applicable 600 28 29 33 27 29 30 26 29 2S 33 100 29.2 7.9
-2.8 800 29 30 27 27 29 29 27 26 28 27 100 27.9 4.6 1.C lo00 32 31 26 29 29 23 23 23 27 24 100 26.7 12.9 6.0 1200 22 24 11 19 7
13 6
5 15 9
100 13.1 51.6 53.9 1400 7
8 14 12 7
7 14 9
9 100 9.5 29.5 66 Dunnettas MSD value:
3.751 PMSD:
13.2 MSD -
Minimum Significant Difference PMSD -
Pecent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimun percent difference between the control and treatment that can be declared statistically sgnificant in a whole emuent toxicity test. On aveage a significant difference occurs c
for Environmental Testing Solutions, Inc. chronic toxicity test when a loxicant reduces CeiodWhnla reproduction by 9.7% fom the control Lower PMSD bound detennined by USEPA (10 percentile) - 11%.
Upper PMSD bound detenmined by USEPA (90' percentile) - 37%.
The lower and upper bounds wer calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Undesltnding and Accounting for Method Variability in Whol Effluent ToxicityApplications Under the National Pollutant Discharge Elimintion Program EPA433-R404003. US Envitonental Protection Agency, Cincinnad, OH.
Environmental Testing Solutions, Inc.
Statistical Analyses Coriadsphals Sarval and R."redwedea T"t-Reproduetion Stn Dat:
Il/U2005 Tedt aD CdNaC32 SamIp 10 RUE-RcfTaoxicnt BadData:
lIl2005 Lab D.
ETS-E ni. Tat Sol Sampla Tps:
NA0L-qodkm cb1ide SayplD Date:
Protcol FWCHiR-SPA-921-R4L-013 Tea Species:
CDCadplinh dubia 0nMments Coea-ng/L 1
2 S
4 5
7 S
9 10 D.Cibo 31.000 34.000 26.000 30.000 25.000 30.000 25.000 29.000 27.000 27.000 600 21.000 29.000 33.000 27.000 29.000 30.000 26.000 29.000 2S.000 33.000 g00 29.000 30.000 27.000 27.000 29.000 29.000 27.000 26.000 23.000 27.000 1000 32.000 31.000 26.000 29.000 29.000 23.000 23.000 23.000 27.000 24.000 1200 22.000 24.000 11.000 19.000 7.000 13.000 6000 5.000 15.000 9.000 1400 7.000 3.000 14.000 12.000 7.000 7.000 14.000 9.000 9.000 3.000 Traasformz: Untraaszfornad Rank 1-7Tled botlole Coe-rng/L Mean N-MA Mean Ml Mar CV%
N SEam Critical M
a N-Meaa D-Control 28.400 1.0000 2SAo4 25.000 34.000 10.259 10 2t.t00 1.0000 600 29.200 1.02S2 29.2D0 26.000 33.000 7.t76 10 113.00 75.00 23.t00 1.0000
- 00 27.900 0.9S24 27.900 26.000 30.000 4.612 10 102.00 75.00 27.900 0.9631 1000 26.700 0.9401 26.700 23.000 321000 12.160 10 90.00 75.00 26.700 0.9271
- 1 2 0 0 13.100 0.4613 13.100 5.000 24.000 51.579 10 55.00 75.00 13.100 0.4549 01 4 0 0 9.500 0.3345 9.500 7.000 14.000 29461 10 55.00 75.00 9.500 0.3299 Auxillauy Teu Stisc Critical Skew Kart KokoogprovD Teat indicates a1 dleaidr (p > 0.01) 0.742999S5 1.035 0.57349241 1.15S46555 Bartle Teat idiratoo unequal vrananc (p - 1.192.04) 25.3599415 15.0S62722 Hypttheis Tet (1.-tl, LIS)
NOlC LOWC CkV T1U Staa Many-One Rank Ted 1000 1200 1095.44512 Treatments -w D-Conol aaar rlnterpolatlon (200 Resamples)
Point wIlL SD 5t% CL Skew ICD5 390 110.963t05 675.69375 1017.7749
-0.6514 ICIO 1011.47059 45.5475413 t69.119444 1037.63214
-1.6247 IC15 103264706 20.514908 9g3.356136 1059.1445
-1.5647 1.0 IC20 1053.32353 17.4425533 1013.4339 100.63192 40.1269 0.6 I=25 1075 13.7206497 1041.43686 1116.95268 0.2515 1040 1133.52941 26.3539432 1100.74316 1215.26767 1.1061 0.8 IC50 1130.88235 36.6363776 1133.33223 1289.27212 1.1729 0.7 0.0 0.1 0
SOO 1000 1500 Doaa-Respeame Plat 35 30 J 25 120 15 10 11-01-05
Environmental Testing Solutions, Inc.
Statistical Analyses Used for PMSD ealculatlon only.
Cerlodaphola Survival and Repoductloa TeAt-Reproduction ShatDale:
I1/12DD5 Tet tD:
CdNsCCR Samplell):
REP-RafTToxint End Date:
I l/V2005 Lab ID:
ETS-nvir. Testing SOL Smpe Two:
NACL.Sodium chloride Sample Date:
Protocol:
FWM-EPA-P 21-R-02-013 Teat Species:
CD.Ccdodq i dubia Cone-mg/L I
2 3
4 5
6 7
S 9
10 D-Cagzol 31.000 34.000 26.000 30.000 25.000 30.000 25.000 29.000 27.000 27.000 600 2S.000 29.000 33.000 27.000 29.000 30.000 26.000.
29.000 2S.000 33.000 So0 29.000 30.000 27.000 27.000 29.000 29.000 27.000 26.000 2U.000 27.000 1000 31000 31.000 26.000 29.000 29.000 23.000 23.000 23.000 27.000 24.000 1200 22.000 24.000 11.000 19.000 7.000 13.000 6000 5.000 1.5000 9.000 1400 7.000 3.000 14.000 12000 7.000 7.000 14.000 9.000 9.000 3.000 Tmunsforen Umtransfonned I-Taied Cenc-rug/L Mean N-Mean Mean Wle Max CV%
N t.Stat Critical MSD D.Caitxol 2B.400 1.0000 23.400 25.000 34.000 10.259 10 600 29.200 1.0232 29.200 26.000 33.000 7.876 10
.0.4BS 2.2B7 3.751 SO0 27.900 0.9B24 27.9D0 26.000 30.000 4.612 10 0.305 2.2S7 3.751 1000 26.700 0.9401 26.700 23.000 32.000 12860 10 1.036 22B7 3.751 01200 13.100 0.4613 13.100 5.000 24.000 51.579 10 9.328 2.237 3.751
- 1400 9500 0.3345 9.500 7.000 14.000 29.461 10 11.523 2.2B7 3.751 Auxliary Tests StatItc Critical Skew Kurt KolmogwovD Tet indicates nom1 dietritix (p >0.01) 0.742999S5 1.035 0.57349241 1.1584655 Bartletfa Teat indicates unequal vusads (p - 1.19B-04) 25.3599415 15.0862722 wypothesis Teast (1411, 0.SS)
NOEC LOEC CkV TU MSDu MSDP MSB 14E F.Pmb d1 Dunntn Test 1000 1200 1095.44512 3.7506703 0.13206586 767.706667 13.451t519 253-20 5, 54 Treatments ve D-Cantrol 11-01-05
Environmental Testing Solutions. Inc.
Page 5 of 6 Species: Ceriodaphnia dabia Daily Chemistry:
CdNaCLCR #: ' q' I
Day I
v_
Concentration 7
CONTROL I
I 4
nH (S.U.)
I I -5 t_
t_._.,
&
S v W
^ -
{l DO (mg/L)
I 600 mg NaCI/L Conductivity iO (plmhos/cm)
Y.0 1620 fj -
.Temperature (or) 7i.44 I *2 L*.q I Iq.bl I I Zq.I pH (S.U.)
DO (mg/L)
Z
?
S 800 mg NaCl/L Conductim f/
(gmnhos/cm)
Temperature (0c) 2.^
t6 SO 211
'S pH (S.U.).
7 -Y
- 3L
.,_85[
S DO (m/L) 1000 mg NaCI/L Conductivity 2/40 (gimhoslcm) 74oV?
Temperature
-24 R (0C) pH (S.U.)
I I
I I
II DO (mg/L)
I I
H LI" I
H1 1400 mg NaCI/L Conductivity (umhos/cm) j2 3 0 Temperature (C) z4. 1 O' I IL-'
24.1 ii 2'.
2q.I I
II ?-..1 I I
STOCK Conductivity 2I N
_ (4mhos/cm)
A__K Initial Fnl Initial Initial I
Final I
Environmental Testing Solutions, Inc.
Page 6 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:
r2 I
Day I
3 I
4 s--v-J I
rN 5
I I.-
I. -
Concentration Parameter I.
pH (S.U.)
DO (mg/L)
CONTROL t4 WsO Conductivity (umhos/cm)
Alkalinity (m2 CaCO/L)
Hardness (mg CaCO3/L)
Temperature (C)
I I
I I
I I
I I
pH'(S.U.)
+.&O
?le 1_bs75E)lg't1 DO (mglL)
.4 5s
.S C.S1.
,5 C
iw 4 600 mg NaCVL Conductivity' (ijimhos/cm)
/5 IM Temperature s
pH (S.U.)
1'4.
- S 5
F 7./
I6.5 DO (mg/L)
IVCo q.o t.(
.6
-(10
- 9. -(
7 4 800 mg NaCI/L Conductivity P10 I las Vo Temperature IS
.b S3
-2'.
2S 3 pH_(S.UtC7
- 7.
DO (mg/L)
- C
,1^
7.6
+C
.a ln*
1000 mg NaCIJL Conductivity F2.20 20C)
(iimhos/cm)
Temperature e
.Lt
. O 25.O
-t
. O Z5. OC
_CIQ I
pH (S.U.)
DO (mg/LI I
-I W
I- *. O/
19.q-
_ov w
1
{_
v I
I-rI I
1200 mg NaCVL Conductivity (gmhos/cm) k m___
2S.O 6 7..
'7I 29 i'4. b I
zq. p Temperature (0C)
- 24. 1 I zS.o.
,2
^
1
'3
--
4 fl.
4fl.
5.0 DH (S.U.)
I-£ (n 2 II I r.
1 7 r.
- g.,2-- I '-%-A9 I *-
£
!L 11 DO (ma/L) 1400 mg NaCIL Conductivity (1gmhos/cm)s 121 Temperature (0C) z0.J I '2S-Z* I
%@.Y (0-.6 Z2S. 0 v
z4 A 12 0 In-itil
[
ial Initia STOCK Conductivity I
ho I. (Pmhoscm)-)
10 gqio00 0
2s lz.1
-O Fna Initial 4
end Far up'-
PERMITTEE NAMEIADDRESS (Include Facility Nameolocation ifDifferent)
Name TVA - SEQUOYAH NUCLEAR PLANT________
Address P.O. BOX 2000
_QqTEBOEF CE S§? -
SODDY - DAISY TN 373 84 fgjjly_ _A-SEOUOYAH NUCLEAR PLANT_
Locaton HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form)
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB i I
TNO026450 1
103 G F - FINAL I
PERMIT NUMBER I I DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND IMONITORING PERIC EFFLUENT YEAR I MO I DAY I EARI MO l DAY mrr I
Approved.
No. 2040-0004 ATTN: Stephanie A. Howard Froml 05 I 11 I 01 I Tol 05 I l1 1 30 1 NOTE: Read instuctions before completing this fomn.
NAME/TiTLE PRINCIPAL EXECUTIVE OFFICER 11 Carf under penalty of law that is doumxent and all attadwments ware prepared under my a
up -
v-A-aylmsaseul--Ir
{ltq~lllApr~ll lullalun v I J. Randy Douet Site Vice President
- her and evaluate the information submitted. Based on my inquiry of th person or h manage the system, or time persons directly responsible for gathering the the information submitted is to the best of my knowledge and belief, true, id complete. I am aware that there are significant penalties for submitting false including the possibility of fine and Imprisonment for knowing violations.
Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT
.11 IUiIllULUf I, TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)
EPA Form 3320-1 (REV3199)
Previous editions maybe used Page 1 of 1 EPA Fonrn 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilitV Name/Location ifDifferent)
Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 -
_ _ INTEROFFICLE §§?N-2S__
SODDY - DAISY _TN 37384 FadcLy WTA - SEQUOYAH NUCLEAR PLANT Locaion _AMILN COLUNTY.
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TNOO6 450II 107 G l F - FINAL I
PERMIT NUMBER I I DISCHARGE NUMBER METAL CLEANING WASTE POND Form Approved.
OMB No. 2040-0004 I-EFFLUENT l
l l I'll t
l o YEAR TMO I
f1 MO I DA From Os 11 l 01 lTo 05 l 11 l 30 l
- -* NO DISCHARGE x
NOTE: Read instructions before comopetina this fbrrn.
ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 00400 1
0 0
PE2I
~
0 UDIY GA EFFLUENT GROSS VALUE R_______ SU X
m SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 0530 1
0 0
- a*
MGL1AL COfO EFFLUENT GROSS VALUE Now M-Em OIL AND GREASE SAMPLE
- 1******
- e*9**
MEASUREMENT 00556 1
0 0
~M MOIL DLY GA EFFLUENT GROSS VALUE MAt X<
PHOSPHORUS, TOTAL (AS P)
SAMPLE 19****
- 19 MEASUREMENT 00665 1
0 0
WE MOIL
(
. C1OMISI EFFLUENT GROSS VALUE Mp-X L
SAMPLE
- 19***
19 MEASUREMENT 0104 21 00
-,MGIL DALYOMO EFFLUENT GROSS VALUE M--
SAMPLE 19 MEASUREMENT 01045 1
0 0
MI EFFLUENT GROSS VALUE MGIL D
COM FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 5050 1
0 0
~MD~DIY CLT EFFLUENT GROSS VALUE MG DA X
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachrnents were prepared under my ix l
/
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnei l fn
)lm d (-(
persons who manage the system, or thos persons directly reisponsible for gathering the Principal Environmental Engineer 423 843-6700 05 12 14 J. any oue papin lyi ga.theradvlutth information submittedit h bet Basykwed ge m mids oeif, thueprono Site Vice President accurate, and complete. I em aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUIVE TYPEDORPRINTED infonmation. including the possibility of fine and inprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
___________________________ICODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199)
Pirevious editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if DifferenO Name TVA - SEQUOYAH NUCLEAR PLANT _
Address P.O. BOX 2000
{LINTEROFFIE MO-2 ___.
SODDY -
AISY TN 37384 facilt TVA - SEQUOYAH NUCLEAR PANTE Location _AMILTON OLUN)LY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN0026450 I
110 G l F-FINAL I
PERMIT NUMBER I I DISCHARGE NUMBER RECYCLED COOLING WATER IMONITORING EERIOR lEFFLUENT YEAR I MO DAY I YEAR I MO l DAY l
k%
flUAnflff r71i Form Approved.
OMB No. 2040-0004 A*
ATTN: Stephanie A. Howard Froml 05 I 11 01 To 05 1 11 1 30 1 NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 04 04 CENTIGRADE MEASUREMENT 0010 Z
0 0
4'
- DEG, DEGC
~ ~ L~~R5 INSTREAM MONITORING C PH SAMPLE 12 MEASUREMENT 00400 1
0 0
~.-~~--~~~S
~
EKY-RB EFFLUENT GROSS VALUE
-U X
m m
SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1
0 0
EMT~****~3 MGIL DIY CMO EFFLUENT GROSS VALUE 12A LY X=
OIL AND GREASE SAMPLE
- 19 MEASUREMENT 00556 1
0 0
-PR(
0 0
j MGD X AA EFFLUENT GROSS VALUE
~~DIYX CHLORINE, TOTAL RESIDUAL SAMPLE 19 MEASUREMENT 50060 1
0 0
MGIL X
mR EFFLUENT GROSS VALUE 1ALM SAMPLE MEASUREMENT NAME/rITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attacdhents were prepared under my
,1 lI TELEPHONE DATE direction or supervision i accordance with a system designed to assure that qualified personnel I J. Randy Douet prop rly gather ancd ev luato the infomaion submitted. Based on my quiry of the person or v4 parsons who manage the syItematiornmttehoe pson rest olmy knowledge afrd belief, truePrincipal Environmental Engineer 423 8436700 05 12 14 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I_
CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA orm33201 (EV 199)
Preiousedifon maybe sedPage 1 of 1 EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page I of I
PERMITTEE NAME/ADDRESS (Include Facility Nametocation if DihTerent)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INITEROFFICE §S- -
SODDY - DAISY TN 37384 Facility A -_SEQUOYAIH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DUR)
(SUBR 01)
I TN0026450 l
77 1 10 T I F - FINAL I
PERMIT NUMBER I [ DISCHARGE NUMBER { RECYCLED COOLING WATER I
-MlONITRING PERIOD EFFLUENT Form Approved.
OMB No. 2040-0004 I YEAR I MO I DAY I I YEAR I MO I DAY I Froml 05 I11 I 01 I Toj 05 1 11 1 30 1 NO DISCHARGE [ I **
NOTE: Read instructions before con NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and aS attachments wer prepared under my r
I TELEPHONE DATE lirection or supervision in accordce with a system designed to assure that qualified personnel J
ff i0 Q
L A
J. Randy Douet properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or thos persons directly responsible for gatherin M Principal Environmental Engineer 423 843-6700 05 12 14 Site Vice President
~information, the information submitted Is, t0 the best of my knowledge and belief. true, Site Vice President accurate, and complete. I am aware that he are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED-inforiation.
including the possibility of fins and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS.(Reference anl attachments here)
No Discharge this Period EP For 330- (RE 3_ 9 Prvu editions..............
ma be use Page...........
1 o. 1 EPA Form 3320-1 (REV 3t99)
Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility Nameltocation if Different)
Name TA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
____L11l EROFFICjE S-2B___
SODDY-DAISY_
TN 37384
_dib__V
_A-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN0026450 I j 116 G F - FINAL I
PERMIT NUMBER I ISCHARGE NUMBER BACKWASH EFFLUENT Form Approved.
OMB No. 2040-0004 Froml 05 l 11 I 01 I Tol 05 I ii r 30 1 NOTE: Re CHARGE 1 --
,ad instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE 0
- A 0
1/30 VISUAL MEASUREMENT 01345 1
0 0
I R
RT z:.>m; PASSzo SEE EFFLUENT GROSS VALUE EQIM~~M OTAL.
e*i OIL AND GREASE VISUAL SAMPLE 094 0
1 130 VISUAL MEASUREMENT 84066 1
0 0
R0YES I
I EFFLUENT GROSS VALUE MT~~
NO=0 N
PRIT-SAMPLE MEASUREMENT
"<1 SAMPLtE MEASUREMENT
' 4OR SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certy uwder penalty of law thiths docmien and all aftadent wwr prepared under my
/
TELEPHONE DATE direction or supevision in accordance with a system designed to assur that qualified prsonnel tno a I-J. Randy Douet properly gadher and evahlate th information submitted. Based on my inquiry of the person or.
persons who manage tM system, or tho persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 05 12 14 StViePeietinformation, the informnation submitted 13, to fth best of my knowledge and belief, true, 42]4-70 5
12 1
St Vice President acrate, and complete. I am aware that te are significant penaitles for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED inrormation, including the possibility of fine and imprisonmant for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY T Y PE O R P RIN E D C O D E COMMENTS AND EXPLANATION OF ANY vOLATIONS (Reference all attachments here)
Operations performns visual inspections for floating debris and oil and grease during all backwashes.
c^PA For 32-1RE 3/s Prvousv eions may.................
be use Pae1o EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facilitv NameiLocation if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT -
Address P.O. BOX 2000 -
INTEROFRCE SB-28 SODDY-DAISY_
TN 37384 fadcjly V
- SEQUO AH NUCLEAR PLANT Locaton _HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT D0MR)
(SUBR 01)
I TNOO24 117 G I F - FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING eERISEFFLUENT I YEAR I MO I =
I YEAR i MO W
iMO rmet Form Approved.
OMB No. 2040-0004 ATTN: Stephanie A. Howard Froml 05 11I01 Tol 05 111 1 301
[rev iI.
NOTE: Re CHARGE [il...
ead instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX sO TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS DEBRIS, FLOATING (SEVERITY)
SAMPLE******
0 9
0 1130 VISUAL MEASUREMENT 9
01345 1
0 0
j EP~
PSO EE ISA EFFLUENT GROSS VALUE L
FAIL=
ERM OIL AND GREASE VISUAL SAMPLE
- 94*
0 1 /30 VISUAL MEASUREMENT 84066 1
0 0
-- k-EhMI1 7
YE=1*SE VUA R~'alR E EFFLUENT GROSS VALUE NOO
_ERMA m
SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER TI Certify under penalty of law that this document and all attachments ware prepared under my TELEPHONE DATE adyrect ontor supervisiond enaccordance withrasystem desgBned to asure that quaified personnel
.D 1or)JHA()JI h d WAJYM __
J. Randy Douct properly gather and evaluate thre information submitted. Based on my inquiry of the person or ok;vi*rsll
.persons who manage the system, or those persons directly responsible for gathering the Princpal Environmental Engineer 423 83-6700 05 12 14 SieViePrsdetorniation, the information submitted Is,* to the best of my knowledge and belief, tbue, 423__84___6700______2_14 Site Vice President acate and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE l
___ormation, including the possibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes.
EPA For 3301(E_19 rvoseiin a eue ae1o EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of I
PERMITTEE NAME/ADDRESS (Include Facilitv Nameltocation if Different)
Name _ _VA - SEQUOYAH NUCLEAR PLANT Address P._O. OX 2000 (INLTE~ROFFICE 113-0B_
SODDY - DAISY_
TN 37384 Laoyt WA - SEQUOYAH NUC EAR LANT_
Location HAMIONCL UNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT f(DMR)
(SUBR 01)
I TNI 026450 ll 118 G7l F - FINAL I
PERMIT NUMBER I I DISCHARGE NUMBER WASTEWATER & STORM WATER I M dONITORING EERIOI l
EFFLUENT YEAR MO I DA I YEAR MO l DA a n_ rnt*nr Form Approved.
OMB No. 2040-0004 ATTN: Stephanie A. Howard From! 05 I I Il 01 I To l 05 l 11 l 30 l NOTE UIreMraUd I
Ar I NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT 19 0300 1
0 0
- ~aoi ~>
e*a*a**~MGIL T-WIE/
RA EFFLUENT GROSS VALUE J
X Ca-SOLIDS, TOTAL SUSPENDED SAMPLE 1***19**
MEASUREMENT 1
00530 1
0 0
MGUL iCi:
GRAB TRAMETPLNREASUREMENT-......
DAIL MX EFFLUENT GROSS VALUE WEEK SOLIDS, SETTLEABLE SAMPLE 2
MEASUREMENT 005945 1
0 0
7P§ T~
- ~~a~~
'a**
a**~MUlLONE GA EFFLUENT GROSS VALUE REQRi~4NESRMAIMXOT FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1
0 0
t.0 t
MGD q;w~~*~
OC/ETM EFFLUENT GROSS VALUE A
VpDILY BTC SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cery under penalty of low that this doaxiewt and all attachments were prepared under my TELEPHONE DATE directon or supervision in aordrnc with a system designed to assur that qualified personnel J. Randy Douet property gather and evaluate the iformetion submitted Based on my inquiry of the person or ite Vice Presient acurate. and complete. I am aware that there re signifficant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED O R PRINTED Information inudin the possibility of fine and Imprisonment for knowing violations.
OFFICE OR AUTHORIZED AGENT ARCODE YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of I