Text

Sequyoyah Nuclear Plant Discharge Monitoring Report for June 2005
	ML051990397
Person / Time
Site:	Sequoyah
Issue date:	07/14/2005
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML051990397 (116)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 July 14, 2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JUNE 2005 Enclosed is the June 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant.

Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

./ II 35 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pfted on cyc~e W

PERMITTEE NAMEJADDRESS (Include Facilvit NamoA ocat on ifDiffeent)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ____________

_ _ _ _LPEIROMIICE S-0 SODDY - DAISY TN 37 84 Facility VA-SEQUOYAH NUCLEAR PLANT_

Location AMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

Form Approved.

OMB No. 2040-0004 I

TNO026450 J

101 G I F-FINAL I DISCHARGENUMBER DIFFUSER DISCHARGE I

PERMIT NUMBER MONITORING PERIOD I

EFFLUENT From 0

To l NO DISCHARGE Z

F6 I0 NOTE: Read Instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

_EX__

__O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS E

iT TEMPERATURE, WATER DEG.

SAMPLE 28.2 04 0

30/30 MODELD CENTIGRADE MEASUREMENT 00010 Z

0 0

DEGR.Cz..****SEE-CK***

REQ ;+~.

'REQIIREMENT.

INSTREAM MONITORING

'^.

i i

DAILY. MX E

TEMPERATURE, WATER DEG.

EASMPLE 40.6 04 0

30D30 RCORDR PH

~~~~~~~SAMPLE

- - - 74O 110 GA CENTIGRADE MEASUREMENT 00010 1

0 0

M.P--pERMrT,

____-__-__4.

0; 03 RCOR R EFFLUENT GROSS VALUE

RE,`DUIEMEN-MINM T

IMUM CR PH T

SSAMPLE 5

8 19 0

PM/30 GRAB MEASUREMENT 7.4 12 00400 1

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UpERMTŽl-

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WEEKLY GRAB;-

EFFLUENT GROSS VALUE REQUIREMENT...M...

iMAXIMUM,--

7.7 DiX SOLIDS, TOTAL SUSPENDED SAMPLE 5

8 19 0

5/30 GRAB MEASUREMENT 19 EFFLUENT GROSS VALUE EEQUIREMENT

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O

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DA Y-FLOW IND CONUITORSE R SAMPLE15800 3030 CRD TETETPATMEASUREMENT 51

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PERMIT,:-"-_-.'.

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.IS A

NAME/TITLE PRINCIPAL EXECUTiVE OFFICER I Certif unter penalty of law at tis document and al attachrent were prepared under my TELEPHONE DATE J. Randy Douet ~properly gather and evaluate the Informaetion submtted. Based on mny inquiry of th e person or iJ tC persons who manage the Syte.or irleithose peson irectyi respoansiblie forgthrung Pl al Environmena 84iee 23 13-6700 05 07 1

Site Vice President accurate, and cornplete. l am aware that there are significant penalties for suomitting false SIGNATlURE OF PRINCIPAL EXECUTiVE

......... L...................1 TREATMENT PMnfoAmation Incuding the possibiityof fine and IRonentforknowingvioons.

OFFICER OR AUTHORIZED AGENT ADEA NUM8ER YEAR MO DAY TYPED1 OR PRNE MGD___________________________TI________________R COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No closed mode operation. CCW data for June 2005 Is attached. Veliger monitoring Infomation Is attached.

A m 31 (V

P s en m

e ud P

o EPA Form 3320-1 (REV 3199)

Pirevious editions may be used Page I of 2

CCW TRENCH DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DatelTime Analyst Method 06/03/2005 0950

<0.5 mg/L 06/07/2005 2255 KRR EPA 8015B CCW CHANNEL DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 06/03/2005 0955

<0.5 mg/L 06/07/2005 2217 KRR EPA 8015B

Sequoyah Forebay Sampling

_Water Mean# of Water NOTES: %

Sample Date Mean # of

% Sett Wars Temr.

Sample Date asnati Temp Gavid LOCATION WaSUB SAMPLE COLLECTED BY SettlecAsrsicLOCATION TYPE

(,C)

Clamnswn CC)

Clam 02/16/2005 0

0 7.9 02/16/2005 0

7.9 0

Outside Plant Forebay Quant S Jones-Brier, J Justice 02/23/2005 0

0 8

02/23/2005 0

8 0

Outside Plant Forebay Quant S Jones-Brier, J Justice 02/28/2005 0

0 7

02/28/2005 54 7

0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/07/2005 0

0 7

03/07/2005 82 7

0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/15/2005 0

0 10 03/15/2005 122 10 0

Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/21/2005 0

0 10 03/21/2005 105 10 0

Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/29/2005 0

0 12 03/29/2005 101 12 0

Outside Plant Forebay Quant S Jones-Brier, Chris Coe,JRigsby 04/05/2005 0

0 13 04/05/2005 525 13 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 04/07/2005 0

0 14 04/07/2005 252 14 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/04/2005 0

0 17 05/04/2005 0

17 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/09/2005 0

0 18 05/09/2005 632 18 60%

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/12/2005 0

0 18 05/12/2005 549 18 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/17/2005 43 50 23 05/17/2005 106 23 0

Outside Plant Forebay Quant J Rigsby, M MacCarroll 05/19/2005 31 50 21 05/19/2005 299 21 33%

Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 05/23/2005 579 50 20 05/23/2005 22 20 0%

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/26/2005 242 50 20 05/26/2005 61 20 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 05/30/2005 1530 26 22 05/30/2005 1831 22 66% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/02/2005 1344 30 22 06/02/2005 1989 22 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/07/2005 240 40 23 06/07/2005 613 23 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/09/2005 192 40 24 06/09/2005 425 24 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll, Shaffer 06/13/2005 236 42 24 06/13/2005 928 24 0

Outside Plant Forebay Quant J Rigsby, M MacCarroll 06/15/2005 78 33 24.5 06/15/2005 2491 24.5 0

Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/23/2005 0

0 24 06/23/2005 171 24 0

Outside Plant Forebay Quant W. Allen 06/24/2005 0

0 24 06/24/2005 784 24 0

Outside Plant Forebay Quant W. Allen 06/27/2005 0

0 24 06/27/2005 305 24 0

Outside Plant Forebay Quant W. Allen 06/30/2005 0

0 24 06/30/2005 0

24 0

Outside Plant Forebay Quant W. Allen

Sequoyah IIplant Sam pling SmlDaeMean # of

%Stlr Water Mean# of Water SAMPLE Sample Date

% Settlers Temp.

Sample Date Asiatic Temp.

LOCATION SUB LOCATION SAMPE COLLECTED BY

('C)

Clams/m3

(,C) 04/01/2005 0

0 14 04/01/2005 29 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/06/2005 0

0 13 04/06/2005 81 13 INPLANT Inplant Raw Water QUANT Dick Adcock 04/08/2005 0

0 14 04/08/2005 54 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/13/2005 0

0 16 04/13/2005 106 16 INPLANT Inplant Raw Water QUANT Dick Adcock 04/15/2005 0

0 16 04/15/2005 0

16 INPLANT Inplant Raw Water QUANT Dick Adcock 06/23/2005 432 50 25 06/23/2005 154 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/24/2005 162 80 25 06/24/2005 30 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/29/2005 290 80 26 06/29/2005 168 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/01/2005 318 100 27 07/01/2005 127 27 INPLANT Inplant Raw Water QUANT Dick Adcock

PERMITTEE NAMEIADDRESS (Include FacditVNarmeltocation if DifferentJ Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

____ffENTEOFIEB S--

SODDY-DAISY TN 37384

_acility_ VA-SEQUOYAHNUCLEAR PLANT____

Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR)

(SUBR 01)

Form Approved.

OMB No. 2040-0004 TN0026450 101 G F - FINAL IPT NMBER ISCHARGE NUMBR DIFFUSER DISCHARGE I M ONITORING

'ERIOD L.

EFFLUENT IYEAR I MO I D2AY Il YEAR I MO I DAY M^ ftcrotLAMer r--

...s ATTN: Stephanie A. Howard Froml 05 l 06 T 013I Tol 05 l 06 1 30 1 NOTE: Read Instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE 0 1 62 0

30 /30 CALCTD CHANGE MEASUREMENT 82234 1

0 0

'-PERMflY<

t

}

2 DEG C/HR CONTIN CALCTD' EFFLUENT GROSS VALUE iEQIREMENT-.

f

-2 UOUS' TEMP. DIFF. BETWEEN SAMP. &

SAMPLE 2.3 04 0

30/30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1

S 0

':sPERMIT

'3'0-' '

DEG. C.

-CONTIN CALCTD EFFLUENT GROSS VALUE REURMN0 t

.:L t'~Uo--

BORON, TOTAL SAMPLE

<0.20

<0.20 19 0

1/30 GRAB MEASUREMENT 01022 1

0 0

~t.->PERMIT.'

^w +****+*

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-REPORT,

EPORT-r-MG/L ONCE/-

'GRAB.

EFFLUENT GROSS VALUE MOREQUIREMENTW SAMPLE MEASUREMENT R'EQUIREMENT --1I.

SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT REQU'REM L

SAMPLE MEASUREMENT 4..

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER J. Randy Douet Site Vice President I Certify under penalty of law that this document and all attachments were prepared under nmy direction or supervision In accordance wtilh a system designed to assure that qualified personnel property gather and evaluate the Information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information. the Information submitted Is. to the best of my knowledge and belief. true.

accurate, and complete. I am aware that there are significant penalties for subnitting false Information, Including the possibility of fine and Imprisonment for knowing violations.

rincipal Environmental Engineer T

SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a/l attachments here)

The following injections occurred: 1.PCL-222 Copolymer (max. calc. conc. was 0.019mgQL-iimit 0.2mg/) 2.PCL-222 Phosphate (max. calc conc. was O.O54mgQL-limit 0.2mg/) 3.Blodetergent 73551 (max. calc. cone. was 0.008mg/L-limit 2.0mg/L) 4.H-150M (max. calc. cone. was 0.037mg/Q-limit 0.050mg/L) 5.H-1 50M (methylene chloride extraction method was <0.020mgIL-limit 0.050mg/I)

EP For 330.

_RE Prvu edtin ma be

_e Pag 2

EPA Form 3320-1 (REV 3199)

Previous editions may be used Page 2 of 2

PERMIrTTEE NAMEtADDRESS (Include Faci7ity Name/Locatlon ifDifferent)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

___1NTEROFFIqE S13-?N-SODDY - DAISY TN 37384 Lac y TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR01)

OMBNo.2040-0004 l

TN0~026450 I I 101 T l F-FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 From 0

10_

1 To 10 613 EFFLUENT

- - NO DISCHARGE

=

NOTF: Read Inasnidtions hbeore nomoelino Ihis form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

_EX__

O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE

>100 23 0

1/0 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1

0 0

7;EMT'14.;****4..,.i*** 49*

PERCENT QTRLY COMPOS EFFLUENT GROSS VALUE RQUIREMENT t

IC25 STATRE 7DAY CHR SAMPLE

>100 23 0

1/0 COMPOS PIMEPHALES MEASUREMENT TRP6C 1

0 0

.PERMITA.,

em 1..149 PERCENT QTRLYI! COMPOS EFFLUENT GROSS VALUE

.UREEN MIMINU PV,_C0 SAMPLE MEASUREMENT

REQIJRE~vINT

R'i 1

SAMPLE MEASUREMENT MEAUIREMENT.'

SAMPLE MEASUREMENT

!'REQLUIREMEN10_

SAMPLE MEASUREMENT

-PERMITh 7-t3REQLIREMENT

.P SAMPLE MEASUREMENT NAMETrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and an attachments were prepared under my i/

TELEPHONE DATE irectlion or supervIsIon in accordance with a system designed to assure that qualified personnel k

. !1L,,tJ4.Ad J. Randy Douet properly gather and evaluate the Information subrnited. Based on my Inquiry of the person or persons who manage the systern. or those persons directly responsible for gathering the Pincial Environmental

~~~~~~~~~Informabion.

the Informstion subrnitted Is, to the best of my Vtovetedge and belief. truePicpiEvrnetlEgne 2

4-70 0

7 1

Site Vice President accurate, and complete. I am aware that there are significant penalties for subnitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Information, Inctuding the possibility of fine and Imprisonment for knowing volatlons.

OFFICER OR AUTHORIZED AGENT AREA NUMFER YEAR MO DAY TY EIRP I

T D]________________________I_______________

CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity was sampled June 5-10, 2005. Report Is attached.

EP Fr 330-(RE 3/9 ru edtin mab sdPae1o EPA Form 3320-1 (REV 3f99)

Previous editions maybe used Page I of 1

July 11, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, JUNE, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected June 5-10, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values forboth species were

> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.

Call me at (256) 386-2755 if you have any questions or comments following your review of the report.

Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment):

Files, R&TA, CTR IB-M SQN June 2005M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: July 11. 2005

1. Facility / Discharger: Sequovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3.266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQ10: 2,992.4

8. OutfallTested: 101

9. Dates Sampled: June 5-10. 2005

10. Average Flow on Days Sampled (MGD): 1545. 1553. 1553

11. Pertinent Site Conditions for June 5-10, 2005 H-15OM was injected:

Injection Location I Date/Start Time (ET)

Date/Ending Time (ET)

Essential Raw Cooling Water (ERCW) Train A 06/06/2005 1130 06/08/2005 1130 Towerbrom 960 was injected:

Injection Location Date/Start Time (ET)

Date/Ending Time (ET)

Raw Cooling Water (RCW) 06/05/2005 0000 06/10/2005 2400 Essential Raw Cooling Water (ERCW) Train A 06/08/2005 1250 06/10/2005 1755 Essential Raw Cooling Water (ERCW) Train B 06/08/2005 1250 06/10/2005 1755

12. Test Dates: June 7-14. 2005

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 10.98. 22.0, 43.9. 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0.43.9. 72.0. 100.0 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: ICs, = 43.9%

17. Test Results: Outfall 101: Pimephalespromelas: IC75> 100%

Ceriodaphnia dubia: IC25 > 100%

UV treated Outfall 101: Pimephalespromelas: IC)5 > 100%

18. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected June 5-10, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.

2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/

(MM/DDJYY)/

Arrival Initial (MM/DDJYY)/

Sample Time (ET)

Time (ET)'

Temp.

TRC*

Time (ET)

ID Collected Received (0C)

(mgJL)

Used By 101 06/06/05 0522 06/06/05 1348 2.5, 2.6 t

<0.10 06/07/05 1414 06/05/05 06502t 06/07/05 1414 Intake 06/06/05 0550 06/06/05 1348 3.3

<0.10 06/07/05 1414 06/07/05 0628 t 06/08/05 13233 101 06/08/05 0528 06/08/05 1500 2.2, 2.8t

<0.10 06/09/05 1323 Intake 06/07/05 0647 to 06/08/05 1500 3.1

<0.10 06/10/05 1456 06/09/05 0729 to 06/11/05 1342 101 06/10/05 0629 06/10/05 1402 2.9, 3.2t

<0.10 06/12/05 1407 06/11/05 1320 Intake 06/09/05 0743 to 06/10/05 1402 3.3

<0.10 06/12/05 1407 06/10/05 0643061/532

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 A I.0 0C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.

Inc.) for 2 minutes.

3

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source:

Aquatic BioSystems, Inc.

In-house Cultures

2. Age:

22.75-24.25 hours2.893519e-4 days 0.00694 hours 4.133598e-5 weeks 9.5125e-6 months old

<24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5. Organisms per Replicate: 10 10 l

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101 06/07/05-1414 ET 06/07/05-1358 ET 06/07/05-0941 ET

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101 06/14/05-1321 ET 06/14/05-1300 ET 06/14/05-0850 ET

8. Test Temperature: Outfall 101:

Mean=24.8C (24.3-25.3 0C)

Test Temperature: UV-Treated Outfall 101:

Mean=25.00C (24.4-25.30C)

Mean=24.90C (24.3-25.4 0C)

9. Physical / Chemical Measurements:

Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxvgen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 7-14. 2005 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 10.98%

100 100 100 100 100 100 100 22.0%

100 100 100 100 98 93 93 43.9%

100 100 100 100 100 100 100 72.0%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 90 90 90 Test Solutions Mean Dry Weight (mg)

(% Effluent) 1 (replicate number) 23 4

Mean Control 0.780 0.735 0.830 0.791 0.784 10.98%

0.925 0.771 0.775 0.738 0.802 22.0%

0.812 0.681 0.683 0.801 0.744 43.9%

0.858 0.843 0.728 0.797 0.807 72.0%

0.792 0W861 0.841 0.835 0.832 100.0%

0.818 0.770 0.829 0.907 0.831 Intake 0.835 0.730 0.706 0.526 0.699 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.9%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 7-14, 2005 using effluent from Outfall 101.

Percent Surviving Test (time interval used-days)

Solutions 1

2 3

4 5

6 7

Control 100 100 100 100 100 100 100 10.98%

100 100 100 100 100 100 100 22.0%

100 100 100 100 100 100 100 43.9%

100 100 100 100 100 100 100 72.0%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(% Effluent)

Data (replicate number)._

(%flet I

3 4

5 6

7 8

9 10 Mean Control 29 35 32 27 32 31 31 32 32 32 31.3 10.98%

33 31 35 32 30 33 30 34 33 32 32.3 22.0%

33 35 33 31 28 33 30 33 36 34 32.6 43.9%

34 32 37 33 33 32 32 37 32 36 33.8 72.0%

34 33 34 35 32 32 32 36 35 37 34.0 100.0%

36 34 37 40 30 32 37 40 36 37 35.9 IC2 5 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.9%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = I 00/LCs 0: TUc = 100/ IC25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic! 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 7-14. 2005 using water from Intake Percent Surviving Test (time interval us d - days)

(%Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(% Effluent)

Data (replicate number) 2 l3 l4 l5 6

7 8

9 10 Mean Control 29 31 29 28 27.30 29 30 30 30 29.3 Intake 33 30 29 321 32 32 31 29 30 33 31.1 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = I 00/LCso: TUc = 100/ IC25 7

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species)

(Type / Duration)

Conducted June 7-14, 2005 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2

3 4

5 6

7 Control 100 100 100 100 100 100 100 10.98%

100 100 100 100 100 100 100' 22.0%

100 100 100 100 100 100 100 43.9%

100 100 100 100 98 98 98 72.0%

100 100 100 100 100 100 100 100.0%

100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(%Effuent) replicate nuer)

(w 1

2 3

4 Mean Control 0.822 0.755 0.760 0.768 0.776 10.98%

0.940 0.812 0.788 0.710 0.813 22.0%

0.792 0.762 0.782 0.683 0.755 43.9%

0.882 0.899 0.850 0.765 0.849 72.0%

0.777 0.847 0.734 0.878 0.809 100.0%

0.767 0.866 0.767 0.722 0.781 Intake 0.760 0.843 0.902 0.851 0.839 IC25 Value: > 100%

Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 1 00/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date l Time Duration I Toxicant Results (OC 25)

Pimephales promelas June 7-14, 2005 1341 _ 7-days KC1 0.62 g/L Ceriodaphnia dubia June 7-14, 2005 0950 7-days NaCl 1.05 g/L 8

PIIYSICALICHIEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pintephales pronrelas and Ceriodaphnia dlubia Tests, Sequoyah Nuclear Plant Effuent (SQN), Outfall 101, June 7-14, 2005.

Test Sample ID Temper ture (QC Dissolved Oxygen (mgIL) pi I

...j(i. 5

)

Conductance Alkalinity Hardness Total Residual

________Initial Final Initial Final Initial Final (limhioslcm) (mgIL CaCO3) (mg/L. CaCO,)

Chlorine (mgIL)

Control 24.9 24.6 7.8 7.6 7.75 7.63 301 61 83-24.8-25.1 24.3 25.0 7.6 8.0 7.3 7.9 7.65 -

7.85 7.42 -

7.76 294 308 58 63 80 86 10.98%

25.0 24.7 7.9 7.5 7.85 7.62 285---

24.7-25.3 24.3-25.1 7.6 8.1 7.3 7.8 7.73

- 7.97 7.43-7.75 276-290 25.0 24.6 7.9 7.5 7.84 7.63 274 z22.0%

24.7 -

25.2 24.3 25.1 7.6 8.2 7.3 7.8 7.72

- 7.96 7.45 7.78 268 280 4

49%

25.0 24.7 8.0 7.5 7.82 7.64 251 tI

2.

25.2 24.4 24.9 7.6 8.2 7.3 7.9 7.71

-7.95 7.49

-7.83 245 -

258 2.%

24.9 24.6 8.0 7.6 7.80 7.66 222 24.7

- 25.2 24.3 25.1 7.6 8.4 7.3 8.3 7.70 -.7.92 7.50 -

7.85 211 228 100.0%

25.0 24.7 8.0 7.7 7.77 7.68 193 72 80

<0.10 24.7 25.3 24.4 25.0 7.6-8.2 7.3 8.3 7.65 -

7.91 7.57 -

7.83 183 202 7 1 74 80 80

<0.10 -

<0.10 Intake 25.0 24.6 8.1 7.7 7.86 7.70 192 74 81

< 0.10 24.8 25.3 24.4 -

24.9 7.6 8.5 7.3 8.3 7.70 -

7.95 7.57

- 7.89 180 201 73 75 78 88

<0.10 -

<0.10 Control 24.7 25.1 7.8 7.9 7.75 7.75 301 61 83 24.4

- 24.9 24.9 25.3 7.6 8.0 7.8 8.1 7.65 -

7.85 7.60 -

7.89 294 308 58 63 80 86 10.98%

24.7 25.1I 7.9 8.0 7.85 7.75 285 24.5

- 24.9 24.8

- 25.3 7.6 8.1 7.7 8.2 7.73 - 7.97 7.61 -7.89 276 -290 20%

24.7 25.1 7.9 8.0 7.84 7.74 274 20% 24.6

- 24.9 24.8

- 25.2 7.6-8.2 7.8-8.2 7.72 -

7.96 7.61 7.89 268 280 24.8 25.1 8.0 8.1 7.82 7.75 251 24.7-25.1 24.9

- 25.2 7.6-8.2 7.9 8.2 7.71 71.975 7.63 -

7.89 245 258 72.0%

24.9 25.1 8.0 8.0 7.80 7.76 222--

24.8 25.1 24.9 - 25.3 7.6 8.4 7.9 8.2 7.70-7.92 7.64 -

7.90 211-228 10.%

25.0 25.1 8.0 8.0 7.77 7.77 193 7280

< 0.10 10.% 24.8

- 25.2 24.9 -

25.3 7.6 8.2 7.9 *-

8.2 7.65 7.91 7.66-7.91 183 202 71 4

80 80

<0.10 -

<0.10 Intake 24.9 25.1 8.1 8.0 7.86 7.77 192 481

<0.10 24.7 -

25.2 24.8-25.2 7.6 8.5 7.9 8.3 7.70 -

7.95 7.65 -

7.92 180 201 73 75 78 88

<0.10 -

<0.10 Overall temperature ("C)

Pimep hales promelas Ceriodaphnia dubia Average 24.8 25.0 Minimum 24.3 24.4 Maximum 25.3 25.3 9

PIIYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pimneplhales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, June 7-14, 2005.

Test Sample ID Temper ture C0C)

Dissolved O ygen (mg/L) pH (S.U.)

Conductance Initial Final.

Initial Final Initial Final (pmhos/cm)

Control 24.9 24.7 7.9 7.4 7.80 7.62 294 24.7 - 25.2 24.3 - 25.1 7.7 -

8.2 7.1 7.9 7.68 - 7.90 7.50 - 7.74 286 -

309 10.98%

25.0 24.7 8.0 7.4 7.81 7.62 287 24.8 - 25.2 24.3 -

24.9 7.6 -

8.2 7.1 7.9 7.71 -

7.91 7.50 - 7.71 282 -

293 25.1 24.7 8.0 7.4 7.80 7.63 276 22.0%

24.8 - 25.3 24.3 - 25.1 7.6 -

8.3 7.2 -

7.8 7.71

-7.91 7.50 -

7.78 272 2 282 25 39/

25.1 24.7 8.0 7.5 7.80 7.63 256 43.9 24.8 - 25.4 24.3 -

25.1 7.6 -

8.3 7.3 7.8 7.71 - 7.91 7.50 - 7.77 249 -

263 72.0%

25.1 24.7 8.0 7.4 7.79 7.63 225 24.9 - 25.3 24.4 - 25.1 7.7 -

8.4 7.1 7.8 7.71 - 7.91 7.50 - 7.75 221 -

232 100.0%

25.1 24.8 8.0 7.5 7.77 7.67 196 24.9 - 25.2 24.4 -

25.3 7.7 -

8.3 7.1 7.8 7.68 - 7.91 7.50 - 7.78 190 -

208 Intake 25.1 24.8 8.2 7.5 7.82 7.65 195 24.8 - 25.4 24.4 -

25.1 7.8 -

8.4 7.3 7.8 7.73 - 7.93 7.51 - 7.83 189 -

211 Overall temperature CC)

Pimep hales promelas Average 24.9 Minimum 24.3 Maximum 25.4 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected June 5-10, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.

11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales Promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360.1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8. Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by EPA Method 330.5.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KC1 or NaCl.

2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas.

Sodium Chloride (NaCi crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimatin2 the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine,

Diffuser Discharge Concentrations of Chemicals Used to Control Growth

of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,.

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 09/08/1998 9!$:0.015 09/09/1998 'X02o0 09/10/1998 0144>2tS2^r; 09/11/1998,'0.

13,k 09/12/1998.

'0O

_i 09/13/1998 XtO.O°1 09/14/1998 r6d'2 0 02/22/1999 L20.O0124 02/23/1999

-.0'0-' q 02/24/1999 Aooolr9gV 02/25/1999 02/26/1999

-0 02/27/1999 '

OOOA 2f 02/28/1999 o:oort-2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -June 10, 2005 3

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 10/06/2002 0

10/07/2002 a

10/08/2002 10/09/2002 ql' 10/10/2002 N

10/11/2002 4

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 5

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12i 1998-June 10, 2005 6

Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1__ of 3_

Clicnt: TVA Environmental Testing Solution, Inc.

Dclivcred By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street.

FcdEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify):

is

>)

Facility Sampled: Sequoyah NP 28801 General Comments:

C,7b-c l SeALS OTOCl SAVPL NPDES Nuber: TN0026450 Phone:

828-350-9364

&e i,)

. 6ccb CaoNIT7NoI.

Collected By: Wanda Allen Fax:

828-350-9368 Field Identification /

Grab/Comp.

Collection Date/Time Number FlOW M.RnEe?.

Sample Description Number&

MGD Rain Event?

V.....

Volume (Mark as Appropriate) v CollecteCd C.......ected

-H Date Ti Yes If Ye, No Trace: ;.TS Log AnaTime tApper

____________InchesNune CJsc SQN-101-TOX Comp 6105/05 - 6106/05 0 2 (2.5gal)

NA V

=

]

SQN.INT-TOX Comp 6/05105 - 6106105 Of7/

1 (2.5 gal)

NA 5.r.

j

.J...

Sample Custody - Fill In From Top Down Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time Wanda Allen 06/06/2005 Express Courier 06/06/2005 A1aJc 4Ld-t&j 09415 IF. ~~- M Express Courier 06/06/2005 j

ETS

/

06/06/2005 L

+

t Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. 13iomonitoring samples are preserved by storing them at 6C and shipping them in ice. Trhe hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time or collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivcry or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 2_ of 3_

Client: TVA Environmental Testing Solution, Inc.

Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street.

FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Soquoyah NP 28801 General Comments:

NPDES Number: TN0026450 Phone:

828-350-9364 LAAc.

t._ ooc( b Collected By: Wanda Allen Fax'-

828-350-9368 Field Identification/

CrOab/Comp Collection DatTime Contniner Flow

?

Sample Description Number &

(MGD)

Rai kn Appropite Volume (Mark as Appropriate) sq tbltrY(~t.

~~~~~~~Collected

!..i.

i;i

'es t

'j i

?;-

Date Time yCs

lftYed, l

No l a

-my Tep:

ABy Tim ip SQN-1 01-TOX Camp 6/07/05 - 6/08/05 00 2 (2.5gal)

NA f

SQN-INT-TOX Comp 6/07/05 - 6/08/05

/

17 1 (2.5 gal)

NA V

^

. it'.to'.

Sample Custody-Fill In From Top Down Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time Wanda Allen 06/08/2005 Express Co 06/08/2005 Express Courier 06/08/2005 3, f71A

,,B 06/08/2005 IsCO

/

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. IDiomonitoring samples are prcserired by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time fi-amc.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CIiN OF CUSTODY RECORD Page _3_ of _3_

Client: TVA Environmental Testing Solution, Inc.

Delivered By (Circle One):

Project Name: Scquoyah NP Toxicity 351 Depot Street FedEx UPS Bus Client P.O. Number: N/A Ashville, NC Other (specify): Express Courier Facility Sampled: Sequoyab NP 28801 General Comments:

NPDES Number: TN0026450 Phone:

828-350-9364 C CIl 0 0'L

-A.0&

S 0J -

a01 ;oot C0,010 Collected By: Wanda Allen Fax:

828-350-9368 dl Field Identification/

Grab/Comp Collection Date/rime Container Flow L

Sample Description Number &

(MGD)

Rain Event?

5, B

Volume (Mark as Appropriate)

Collected sDate Time Y

If YesN No Trace S

Arvt Temp By Tim Appear-:

SQN-101-TOX Comp 6/09/05 - 6/10/05 672 2 (2.5gal)

NA t

j ti.iZC-w X

SQN-INT-TOX Comp 6/09/05 - 6/10/05 t1 1 (2.5 gal)

NA

=0CSl Z

Sample Custody-Fill In From Top Down Relinquished By (Signature):

Date/Time Received By (Signature):

Date/Time Wanda Allen 06/10/2005 Exprcss Couri 06/10/2005 Express Courier 06/10/2005 06/10/2005 t'0.

. ~' '02

/1"17.

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samplcs are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.

Samples shipped overnight on Friday via FcdEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

rnvironmental Testina Solutions. Inc.

Page I of 6 1nvironmefltal Testin2 Solutions. Inc.

Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: TVA Facility: Seauovah Nuclear Plant NPDES #: TN 0026450 Project #: -T-ziV't-County: Hamilton Treatment: Non-treated Outfall: 101 Dilution prepar tion info mation:

Comments:

Dilution prep (%)

10.98 22 43.9 72 100 Effluent volume (mL) 274.5 S50 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:

Test information:

Organism age:

22.-IS -ro 2-.-ZS q eU Randomizing template:

,lfm a

Date and times organisms tOa-QIc 4S t330

%S00 Incubator number :

were born between:

Organism source:

frC..ik O6b0-1 OS Artemia lot number !

Transfer bowl information:

pH =

Temperature =

°C Total drying time:

S3 2'4.8 at Date/Timein:C& 1 '15 13L4S Average transfer volume:

Li q.

D Date / Time out:0 6-IS i

Oven temperature:

k'c Dailyfeeding and renewal information:

Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time Igs 0

Cb6-zS

____llA 06-UAcisO i 07-oO 1

io<

clSSZQ Icco

<)Ao-oS J eovco2.4s 2

not-os0 0 c95s I Isss 1-i c3w3 o-04 5 C. OScrOO.&z z

h 3___

I

-4Cso C.-VA Cr vSeaA-.72 3

s 6-12-o¶5 Q RS.

ISoo Ol 1 34-OS G QSob.

16. L-AGC 6

06 1-G5d' Oiso 1S6.!

521!

06Q 01t0516 ioc*4 r

6 640 Control information:

Acceptance criteria Summary of test endpoints:

%Mortality:

s 20%

7-day LCq0

__7_

Average weight per initial larvae:

I.18LI

-*;k NOEC 10b%

Average weight per surviving larvae:

O.181 2' 0.25 mglarvae LOEC

> lot,07 ChV' 15 lC£4 CIS

> Ic o;

.,Environmental Testing Solutions, Inc.

r:.'.:

Species: Pintep halespromela.

lTi Cltent:* TVA ISequoyah N'uclea Page 2 of 6 ar Plant - Non-treated Date:

tol-o-1-OS Survival and Growth Data Day CONTROL 10.98%

22%

A B

C D

E F

I G H

I J

-1 L

IC).

it) 10 to 10 10 I

10 10 I_

lo l C /0 10 /o /0 /0 10 l t 10 /0 14 1-10 10 10( 10

/

/6 1010 0 010 3

MO 10 1O I~ /0 IC) to to G 0 I _

IC I_

_ji b0 t/

io to

/0

/0 /0

'0 5

it-

__/0

/

o lo

/0/0 10

/

10_I 0/0 6

10 t0 IC)

IC) 1 o0 10 0

/0

'~

/R

/0 7

to I-Iv-i 2jjr I___

/

0j

/0/10/10/0 I

o 1

A -Pan weight (mg) e Color Ident'i on: _OAp! lqso SO,1 a.q4 l1 1"v,l Ii91i.3? z 4 q1 1 K.oq

,15.t lq. 1 IS.o 1t4.5B Analyst:

B - Pan + Larvae welght (m~e)1rt ZZ t.30 U.7e Z1, 22-1 -2.

ZS3.6 T02 z; q 2

2

,-L4 4

1 1 Z3.21 lCi 21.01 tbt 2Z-Sq Larvse weight (mg) - A - B 1.30 1.?S 8.o0 1A it.s In k 1.15 1.3k 8.12. b t

.A I

ZV5d Weight per Initial number of larvae (mg)

- C / Initial number Of larvae

%0' 0*

f3Ž'~ Ifo~

Average Percent weight per reduction 0

Initial from control o,18q number of

(%)

0.,0

- -.,I,.

-6. I

-7.

Comrfment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.

Calculations and data rcviewed:-

Comments:

'I.

- EnvIronmental Testing Solutions, Inc.

Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:

ie-,L-M~-CrS

...}

" i~t i,4 tI--,

I.s..

I.&-'..-

.I:--.

l I-I.,t..3......

Survival and Growth Data Day 43.9%

72%

100%

l N

0 O

P Q

I R

S T

U V

W X

ICI__

1 0 0 1/0

1) 10

/0 O 1C) 10/ O 10/0 z(01 010 0/01 0

'0/)I C

D oS}

/010C)

/0

/0/

10 10

/01/01 0 6

to

/

/O /0 3_

0

/_

_Oi /0

/O

/o

/lb 1

_10 3

t o lo 1/()t 1/( lO o IC) Io:

Io lo IC) /O 5

5

.10

/0 lO 1O

/O 10 t

O 10

/0 10 0O 6

/

/0/0/0

/O

/C 7

I(

lo 1L 1O

/)

/L

/O

/L

/

IC)

/

Afl A -Pan weight (mg) &AU(

Color identlficatlon 6eA144 t; Id tS Nq 1 1

S."A jq,98 Isml l,

I.11 1

1.

q V53 t15.Io 1L4.31 Analyst:

AA.4

~

B-Pan+Larvae weight Analyst:

-t

-Z Larvae weight (mg) - A - B O.58 1.^1 a 7.z8 -1.9 42. R.('1 E.&AI j.35 -.12 1.10) 8.sl 9.01 Weight per initial number of larvae (mg)

-Clinitiai nunber orlarvae 1 0*

b0

)*

9.° Average Percent weight per reduction Initial from control 0.80-71 2,91o

0. 3

-bO2l number of

(%)

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

4Envi

1*,-.

*

V UI..

I ronmental Testing Solutions, Inc.

Page 4 of 6 Species: Pimephalespromelas.

Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:

of.-o1-Os Survival and Growth Data Day 100% Intake Y

Z l AA BB 0

IC)io 2

10 10 10 101 3

lo 0l 1, 0 1

0 1 0 I C I C )id 10 90 0 1 4_

IC) lO 10 J S__lO_

10 10

'd 1

0 A - Pan welght (mg) 6LACK Color Identification:_

F_._14 s.I A nalyst:

t 4 4

.I 4

,q q B - Pan t Larvae weight Analyst:

21dc Z.ZtS 21.-1 tZO.22.

Larvae weight (mg) - A - B 935 1.30 1.0b <.52G Weight per Initial number of larvae (mg) 4 AP t°'

ClInitilanumber of larvae Ob Average Percent weight per reduction q

Initial from control 6(Dq'1 number of

(%

larvae (mg)

Comment codes: c clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

TVA / Sequoyali Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Chronic Whole Effluent TosicltyTest (EPA-821-R-02-013, 1I'ethod 1000.0)

Species: Pimephatlexpromelaz Quality Control Verification of Data Entry, Calculations, and Statistical Analyses calet:

TVA / Sequoyal Nutlear Platt, Outft3l 101 Not-trted Tesit datts Jut. 0114, 2005 Revelwed Project au-btfr it" N4ot fore CopliancoAaesgs tr atrssn

t. Ite atra LI--./

I t

P.h.ew.kiI bi IV..inrh.. ee,tae..I F.. e..i.. e.e 0.

AL. T

t.

I C-_*_tb t7s}

or

t.

Ib f

Mh..

k(h a

IN

hdU i")

r*r k

e

(,4 ts kt.(b.,,..

Or IW *ee0.Dl 1%)

C'-

I0 Coatrol IO.98%

22%

72%

31l N

0

-r R

8 T

V x

30 10 10 10 10 10 30 10 10 10 tO 30 10

_30 0.7s0 0.714 5.0 0.715 I000 0.7t4 5.0 Not applicable 0.830 0.791 0.925 0.302 10.4 0.771 100.0 0.802 10.4

-23 0.775 0.73s 0.309 11.6 i0.6ii 9L.

0.744 9.7 11l 0°t13 0.t07 7.2 0.4 72t tO.O O.tO7 7.2

-2.9 0.721 0.792 0.e32 3.5 O.61

00.O 0.832 3.IS

-2 0.t41 0.813 0.831 6.8 0.770 300.0 0.833

6.

-6.0 0.329 0.907 0841 0.-35 100%

14.71 14.79 14.53 15.10 14.39 14.74 23.06 8.35 7.70 0.35 7.30 106 S 26 Y

10

0 100% latskt z

10 10 35.15 2245 0.730 0.7175 5.9 0.335 0.730 0.706 0.526 90.0 0.69 18.4 I0.t 10 9

14.65 21.71 0.714 nn to 7

14.96 20.22 0.751 I__.

I A

I outran 101:

Dallett's btISD valut:

FbSSD:

Intake:

D eltt's MdSD value?

PMSD:

0.1012 IL9

0. 1304 16.6 MSD -

Minimum Siificant DifTcrence PMSD -

Percent Mimrim Sipifct Dierence PMSD is a nowuure of te preeision. The PMSD Is the ninismim Percent diffnce between the control and troatznt that can be declred etwatistaly ofiflnt Ola eiob eluent toxiityteay.

Oa averenf a sipificast difference oceure for Envirocnrtal TeatingSolution e

Inc chronic toxicity tells wtent a toxicant reduces lmephola es by 14.0% intih ot (detir ed tlhmun reference toxican te tinia Lower PMSD bound detnined by USEPA (1Othl percnte) - 9.4%

Upper PMSAD tbund detemine by USEPA (90th peentil.) - 35*%

Th' lower and upper boonds were clsculated by the USPA usIng 205 tesas conducted from 19 lsbormtores for Flmephater growth in chronic reference toxalant teat)

USEPA. 2000.t ndenatndintand AcocuntingforMMtod Variabiity in Whol Effluent Toxicity Appliations Utder theNations Polutant DishargeElin iation Pror BEPA-833-R-00003. Us Envionmtl Ptedion ACney. Ct-clnat(OIL

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Statistical Analyses LarI Fith Crw1 nsd SanivullTut.7 Day Grwth StartDotw 617 TastID:

PpPP SamplID:

WAiSequoyahNuckr rPbl l0l -Non-treated Rod DstL:

6d141M Lab IID R3-iE Taibg Sol SapDV Tp'.

DMR-Dkbar MoDtatme F.pozt Sampe Dat:

Prhosowt FWJCHR.EPA421.R.02-03 Tat Spees:

PP*Pkonphai prombs r.e.%

1 2

3

£ D-Contol 0.7r00 0.7350 0.e300 0.7910 10.91 0.92S0 0.7710 07750 0.7310 22 0.1120 0.6tlO O6t30 0.1310 43.9 0.2580 0.U430 O7280 0.7970 72 0.7920 0.1610 OU410 0.e350 100 0.2180 0.7700 0.1290 0.9070 Tn"sMm: Uatrbatfnmd I-Tal lk Cae.%

M.1.

M N-Mam Mem Mia Mal CV%

Y.

I-Stat Crhdal MSD M0.

N-Mm D-Control 0.7U40 1.0000 740 0.7350 0.1300 4.985 4

.OOO LOXO 1032 0.832 1.0233 0.8023 0.7380 0250 10.401 4

-0.433 2.410 0.1012 0.8000 1.000 22 0.7u43 0.9491 0.7443 0.61n0 0.1120 9.67 4

0.946 2410 01012 0.8000 ILOO 43.9 0.8065 1.0217 0S065 0.7220 0.2120 7243 4

.0.536 2410 0.2012 02M 1.00O0 72 0.8323 1.0615 0.8323 0.7920 02410 3.490 4

-1.149 2410 0.1012 0.8000 1.00:0 200 0.2310 1.05" 0.1310 0.7W0 0.9070 6U32 4

1.119 2410 0.1012 0.000 1.0000 Amiz"arTets Statlis Critic Ske Kert SlaPro-wtsTathtdkatosnoruldsntutxen(p>o.oi) 0.961619651

.18.4 0.45134646.0.36290127 B

EtrsTntkidictes qualvaram(p-0.62) 3517258167 IS.01627224 H"pOdhItTest(14asL OS)

NOEC LOEC EV TU MSD MSDp MSB SKE F-Prob dt DtmosS Talt 00

>100 I

0.101214624 0.121C0215 0.004329942 QCt03527525 0.3363242SI i It Treaensnb wo D-Conkol Ll.nmr laterpolatim (200 Rnm srpia) i.t.

SD 9S%CL21rp)

Ske.

ICOS

>100 ICIO

>100 ICI

>100 1.0 IC20

>100 DI 1C25

>300 2040

>100 0.8 IC50

>100 0.7 0.6 3 0) 0.2 0.1 0.0

02

-0.2 0

20 40 so 8o 100 120 Dogs.1 Do.-Ruspams.e Pt1 1

0..

0.8 0.7 1-ta11. 0.05 level 0.6 ~

~

o osigriicaee 0t 0.2 O.1 sqe06745dala

TVA / Sequoyah Nuclear Plant, Intake Non-treated June 07-14, 2005 Statistical Analyses LaetJ Gswtk 4SarvtnT mt-7Dyvwtb Sturt Dol 6el2GM=

Test IM.

PpPRCR Scnr'cm TMVA/ ShmbihNckax Phlnt Intake - Non-tvated Ead Dtme 6tl4/2005 Lab mr WZs vk. Tuft Sot S

,ic 1pk e:

DM,-Dbcbarr M-flatig Repuat Samph Dte Pwoocot PWCHR-EA4t21-R-2413 Test Specis:

P1'Fksrpiales ptnekbs Come-%

I 2

3 4

D-Coaft 0.7100 0.7350 300 0W7910 100 0.1350 0.7300 0.7060 0.5260 Trasserse IUfsasr d

1T4Il lsotosk CaC-%

Mena N-lmm Mesa

?.Ml Met CV%

N etSt t

3rkd M.ISD Mean N-Mns D-Conktot 0.7340 I.OXO 0.7340 0.7350 0.1300 4305 4

0.7n40 I.OX t00 OM3 0.919 0.6993 03260 0.1350 hI351 4

1263 1.943 0.1304 0.6"3 0.8919 AsuzkiryTab Statistc 05tkncs Skew Kart shpfno-W&s Testkbdicatesitnmaildertzfutim(p>001) 0.9Z1743MS 0.749 075565406 2306717611 F-Tat kAkstes equial vkin (V - 0.0) 10.7190221 47.46722794 lyMins Tmt(1-bJ4 0.)

AISDe MSDp M.Sa MSI

".Prob dr Homoscedutsw I Teat kidwta no sipnifcant dilffmces 0.130372714 0166291727 0.014365125 0.009002792 0.25331911 1.6 teaftmcb Vs D l-Conrol Uear nlterpolation (200 Remsmples)

Point SD 95%CL!rp)

Skew ICOS-46.254 C10' 92.507-IC15

>100 1.0 IC20

>100 0.9 IC25

>I00 1C40

>100 0.8 IC50

>I00 0.7 mdMt3 IC ent ate less Uran te bkiest cmcutstm

0.6 o.s

~0.4 0.2 0.1

.0.0 0

20 40 s0 80 100 120 Dose %

D.s..Iapeo FPbt 9.11 0.8 0.7

= 0.6 0.5 a

" 0.4; I 0.3.

02 0.1 I.............................................................

I1-(aO.0.5 levI of s~gnifcarce I'a xqs_C"6-07-05dof*

Environmental Testing Solutions, Inc.

Page 1 of 7 Environmental Testing Solutions. Inc.

Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia Client: TVA Facility' Sequovab Nuclear Plant NPDES #: TN 0026450 Project #:

'8°A County: Hamilton Treatment: Non-treated Outfall: 101 Dilution p a

n information:l Comments:

Dutionprep(%)

10.98 22 43.9 j

72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:

Test information:

Organism age:

4 z4-Aoun Wt' Randomizing template:

A Date and times organisms O-06-o-o.

-TS VZ...

Incubator number and _

were bom between:

shelf location:

____\\

Organism source:

OS-N-OS A-A YCT batch:

OS145lcs Transfer bowl information:

pH = 1. t1 Temperature 3 z Selenastrum batch:

Daily renewal information:

Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time O

Coorcn-a Ogg I 06-eq-I OS00601 4-to 07 -C>w 2 oib-Q*

Ss R 1Q.

f o~-c-Ss C. c~o io. zz

z3 At 3__

^t>~-cbS n5 L.

QX->CS tt G

~ob.Zd Z3:

4 GM-CR - Os9u 65 1 O~.

0

-A-l A6 aow.b z

A 5

6_c____S O90o '

06-L 6

GSC 5olmg C i 3

Z' 6

__l_-__

o09'1

Ob-o) $_

015ot&..V.3 At Control information:

I 2

Acceptance eziteri Summary of test endpoints:

% of Male Adults:

O2.

s 20%

7-day LC50 7' I7o

% Adults having 3 Broods:

io1r, 1C6'.

f 80%

NOEC l CX)'t Mean Of Espring/Fcmale:

SY3 29 -3 15.0 offspring/(emale ChV 7 lOOT.

% CV:

s.

R7.

<40.0%

IC25

> IG0fo 6

IR__

__0

Environmental Testing Solutions. Inc.

Page 2 of 7 Species: Ceriodaphnia dubia Client: Seguova hNuclear Plant - Non-treated Date:

O-

-05 CONTROL-1 Survival and Reproduction Data Replicate number Da 1

2 3

4 5

6 7 18 9

10 I

Young produced n

Q O

0 OQj O lO o

Adult mortality L-L-X-l t-2 Young produced C0 Cb t

E C

l(

)l Adult mortality l t L.

3 Young produced o_

_i n

I 1

i

.c C) _

Adult mortalihy L-'

L L-l 4

Young produced

_ 5 5 I 5 Adultmortality C

l l

l s

Young produced 1LQ.+/-z 0

+/-LIL 110 jL O ii

.j..

Adult mortality l--

L l

L l-t-

\\.-

6 Young produced l

o

-O a

a O

Adult mortality lQ 4

l_

L 7

Young produced Lk I

l I

ll 1=1 l5 a

1a Totalyoungproduced.

l Z9 I 3Z Zi 3Z 1

31 3Z l

Z 3

Final Adult Mortality 1

t-L X for3' Broods I

X l

C I

I%

Note: Adult mortility (L - live, D - dead)

Conceitration:

l%Mortality I C7 I Mean OffspringIFemale:

1.

CONC: 10.98%

Survival and Reproduction Data i i_

Replicte number D_

3 l

6 l

7 9

10 il Young produced Og I

c l

Adult mortality L

L L

.L-.

j Young produced C_)2<

4k 3 9

1 3

Young produced l

C 0

C)

I I

I aQ-Adult mortality l

L l

a a-a

=

4 4

Young produced l

L L

l 5 SI s

I s I.

I

. I s J

Adult mortalIty L...1 al L

a t_

I S

Young produced l

l\\

l l \\

l

=

1_° Adult mortality l I.

1 1

I 6

Young produced l

OO 1.. 0 4

Adult mortality l

L L

ll L

l 1

7 I Youngproduced 15 l 1

I1s IM 1 kD l LO 11 j 1-I Total young produced 3

313 351 a3L.3o.33 l 3o 3 5L l 3 l Final Adult Mortality t*Cl l

l l

I l

Note: Adult mortality (L - live, D - dead)

I Concentration:

%Mortality:

0 I I Mean OffspringFemale:

7 32-3

% Reduction from Control:

-3.2Z7.

Environmental Testinz Solutions. Inc.

Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date:

Q!6-01-QS CONC: 22%

Survival and Reproduction Data Replicate number Day I

l 2

3 4

5 6

7 8

9 10 Q

0 G

O O

O Adult mortality U

U U

U.

2 Young produced l

C)_

Q Q Q

Q O l

-V I

I - I I 1-I -

aIAdult mortalityU U.

U U

3 l Young produced looI Adult mortality

.c 4

Young produced S

1 5"

57 l

Adult mortality I

U U 1 j

J U

U I l 1I'l t

l S

Young produced r,

z C-)

5 i i ii lZL4i 7t Adult mortality L -l 1

L UIU J

ll rL 67 i

E T-lonpoue ol

~.

.Q.. L~.

o I o I o I

. Tol.~>o lAdultmortslLty JU1 U

U jL

l.

U_

U.

1 Young produced I IS l I

(.l I

LI l

1

-Io I

l Total young produced l 3 3 l 5T 3Z l

33 T

I aL Final Adult Mortality U

U U

7_lll Note: Adult mortality (L

= live, D - dead) l Concentration:

% MortalitY:

j° I Mean Offspring/Female:

l 32.(

l

% Reduction from Control:

- 4.V7.

Co.NC: 43.9%

Survival and Reproduction Data Replicate number Day l

I 2

3 4

5 1

6 l

7 8

9 10 i

Young produced l

I** 0

.Q Adult mortality U

U.

I U

[ U U I U.

U.

A 2

l Young produced

()

o l

Adult mortality U

t l

ll 3

Young produced 0

(1 C):r---

-e i

-I l

lAdult mortality C

f

_ll L-I 1C7 4

Young produced [5 s(F I is 1 6

7 s I l

i Adult mortality

[

U l

l-lt__

k**

U U.

UJ S

lYoung p

rodued

-t l

I I

1 3

Adult mortality C

U U.

U.

6 Young produced l

Ql I___Adult morta t I

_L Total young produced 2:3.

1 T3I3.33 I3Z. j3Z 3

3-1 3a t3 l Final AduIt Mortality Note: Adult mortality (L - live. D - dad) l Concentration:

%Mortality:

07,0 Mean Offsprine/Female:

I 33. 8

% Reduction from Control:

-8. 07.

Environmental Testing Solutions. Inc.

Page 4 of 7 Species: Ceriodaphnia dubia Client: Se uovah Nuclear Plant - Non-treated Date:

cL-,-moS co-4c: 72%

Survival and Reproduction Data Adl K

Replicate number=

Day 1

2 3

4 rS 6

7 S

9 10 Young produced 7 C)

Q lAdult mortality Ilt lL@L

@Ll l-t L-Autmortality 2

Young produced TI Adult mortality

T 3

Young produced Qc

-Q a 0

Adultmortality L-*

L L

L --

L s

Young produced

-L I

L ST Adult mortality l L

l.

l S

Young produced l1 C)

C. 1 0

l 31l Adult mortality L-L

. J 1_

L..

t'- *l

.~

_1~

I Young produced 1

8 O

C-(i O li Ili 1

ick Total young produced l 3'-

b lz za

32. z tu as 1 35 Final Adult Mortality L

c 1

T l

1 l

ll Note: Adult mortality (L - live, D - dead)

Concentration:

%Morialty:a Mean Offspring/Female:

l 34. C)

% Reduction from Control: l -

V7.

CONC: 100%

Survival and Reproduction Data Replicate number Day 3

1 2

T 4

l S

6 7

9 10 j Youngproduced C )

O O

°' T O

I ° l l Adultmortality pt l

l l-l l2 lYoung produced lCB C)O C)~

%l t1 5)IOIO Adult mortality l

\\-

I.-

\\l 3

Young produced C)

(O lOL.

Adult mortality Cl T

L l

l L

L_

LL l

. 1 Youngproduced l

b lAdultmortality 1

L 1

+/-IJ Youngproduced j

3 j10 ii i

3 Adul mortality L

L1 ll-L l t l

i6l Young produced l lO l8lO C) o nt C)

C) l1 Adult mortality l

.l tl 7

Young produced lIi 0

18 ZO j(

I i

)1i C

° Total youngproduced I3CT 3q

.5 1 T4l 30 T 3Z 31 l cy 3b l1 Final AdultNloaltv I.

I I

lW IoItliI Note: Adult mortality (L - live,

- dead)

Concentration:

I Mortality:

o C

r.

lMean Offspring/Female:

35.9 l% Reduction from Control: l-1 t.-17 7

Environmental Testing Solutions. Inc.

Page 5 of 7 Species: Ceriodaphnia dubia Client: Seguovah Nuclear Plant - Non-treated Date:

c..-Oi-cnS CONTROL -2 Survival and Reproduction Data Replict-number D

A r

1 2

3 4

5 6

7 8

9 10 I

Youngproduced cO a

t) l C

l v

Adult mortality L

L-l L

l -

2 Young produced C

O l O l0.

QC)2 o

o~o o

j Adult mortality I I \\J

'I 3

Young produced edr Cl c

Adult mortality l

4 Young produced A3 5

l L

Y p

Adult mortality Lt J _.

U.

L -.

\\_-

l 5

Youngproduced l l

I O. I I

i I w I

0o( 1 Adult mortality l-L I

l 1

U L

L-L-

6 Young produced jC)

I j*

O O

0 j

O 10 l

Adult mortality l

U.

l l

L

\\.

l I

ll 7

Young produced I'S I

I cA I I I

11

%L>o I

Total youngproduced

' 29 2

Z-1

-3 Z9 1

3-

=5 a

.'O Final Adult NM9rtality ICl~--

Note: Adult mortality (L

live, D - dead)

Concentration:

% Mortality:

I 0C7 Mean Offspring/Female:

lA z

_ Inx--

t nno/

rew j

n -P

LUNU: lUU /0 RUL4RX i"I IV& V" va AV JreVa"LL&&l v1aaU Repliet. number Day l

1 2

3 4

5 6

7 l

9 j

10 I

lYoung produced l C) o O

C a) lR C)1t lnlO 1

Adult mortality 2

Young produced

)

[3 Youngproduced C).

C)

C )

Adult mortality IL U-L-

L 4

Young produced 5lI L I L l Adult mortal!tr l

j r

U l

L-l

.I7 J

4 Young produced I

5 C

I C) l C)

Adult mortality L -

I L -

l_

T l

k

\\.-

Adult mortality L-L---

L 7

Yung produced I S I a I 6 I

I

1.

Totalyoungproducduced~

V I Z.j0 lO O

1 29 tO 05 l nalAdult Mortality 1C TlL-l Note: Adult mortality (L -live, D - dead)

-* Co wo&,* f'AAz ot GC54 j

Concentration:

%Mortality:

I 6-7 I Mean Offspring/Female:

131.1 I % Reduction from Control.

- 6.17. 1

TVA / Scquoyalh Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Verification of Cerlodaphnla Reproduction Totals Control-1 D__

Replicate nunter Total Y

1 2

3

_4 5

6 7 1 8 9

1 10° I

0 0

0 2

0 0

3 0

0 0

4 5

5 4

4 5

5 5

6 48 5

10 12 10 9

-11 I

10 10 12 9

104 0 0 0

0 0

0 0 0

0 0

14 18 18 14 16 16 16 17 15 17 161 Totl 29 35 32 27 32 31 31 32 32 32 313 10.98%

Day Re lcate numberToa I1 2

3 1 4 15 7

8 9

10 I

0 0

O O

0 2

0 0

0 3

0 0

0 4

5 4

5 5

6 5

5 4S 5

Il I1 14 12 12 12 9

0 12 10 103 6

O O

0 0

0 11 0

I 7

18 1S 17 15 14 16 16 17 16 17 161 Total 33 31 35 32 30 33 30 34 33 32 323 22%

D Replicate nunber Total r

I 2

3 1 4 3

5 6

7 8

9 S toe I

0 0

0 2

0 0 0 0

0 0

3 0

0 0

4 5

5 5

5 4

.5 41 5

6 1

1 48 5

13 11 12 10 I 10 11 12 12 141 121 117 6

0 00 0

0 0 0 0

0 0

0 7

15 19 161 16 14 17 14 16 16 181 161 Total 33 35 33 l31 l28# 33 30 33 36 34 326 43.9%

I Day RReicate nunter l

21 I

1 2

3 4

5 6

7 1 8 T ta l

I 0-l O I

° I o O~'-

I o

lo I

o 2

0 0

O 0

0 0

0 3

0 0

0 4

51 5

4 S

5 5

5 5 4

61 48 5-1 13 I

1 1 141 12 12 13 12 14-13 13 127 6

0 I

0 1

O 0

O I

7 16 16714l9516l 162 Iotal 34 32 37 33 33 321 321 37 32 36 33 72%

ID I

R. U~ctenumbtr T t I

l Y l I1 2 1 3 1 4 1 5 l6 1 7 1 a 9

I 10ll I,

lo Ol O

10 O

O 0

O IOl o o O

2 0

0 0

3 0

0 0

0

-06 0

0 4

5 5

4 6

5 4

4 5

48 5

1 11 111 13 12 12 1~l0 10 15 13 13 1l20 6

18 0

0 0

16 0

0 0

18 O

52 7

0 17 16 isI0 16 17 17 0 I 19 120 Total 34 33 34 35 1 32 321 32 1 36 35 1 37 340 100%

Day 1

ReplIcate nunber Total 1 2 3 1 415 6

7 1 8 9

10 1

0 0

0 1 0 0

0 0

2 0

0 0

3 0

0 0

O 0

0 0

4 6

5 S

6 4

5 5

6 5

5 52 5

1 13 11 14 14 10 10 15 14 12 13 126 6

0 18 0

0 00 0

20 19 19 76 7

1 7

0 18 20 16 17 17 0

0 0

105 Ttal O363437 40 301 321 371 40 36 37 359 Control-2 Day l

Rlilcte nunmer Tehl 1

2 3

4 5

6 7

8 9 1 10 I

0O o

O O

0 0

O 0

O I

° 0

1 0

0 0

0

°

° 0

0

-4 4

3 5

4 3

5 5

4 4

4 41 5

11 11 9

0 10 10 11 9

10 12I 93 6

0 0

0 10I0 0 1 0 I

0 0

01 10 1

7 14 717 1-5 14 14 15 13 17 16 141 149 Total V2 31 7 2V) 28 27 930 2

30 30 30 I

293 100% Intake i

Dl RIcate nun-er Tet l

l 2

1 21 3

4 l5 l

d 7

l8 l9 l10l l

I 0

l 0

O O O O

O 0

2 0

0 0

0

° 0

0 0

3 0

0 0

00 0

0 0

0 4

5 3

4 6

5 5

4 4

46 5

I13 10 0

10 0

12 10 0

0 it 66-6 0

0 12 0

10 0

0 10 10 0

42 7

I sl 15 13 16 17 15 17 15 16 1 18 157 1

Total 133 30

2) 32 32 32 231

=2)3033 311 1

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client:

TVA / Sequoyah Nuclear Plant, Outrall l01 - Non-treated Test dates:

June 07-14,2005 Project number 1899 Reveiwed by:

(7

/-_

Concentration Replicate number SurvIval Average reproduction coemckft of Pervnt reducton frm

(%)

1 2

3 4

5 6

7 l

9 10

(%)

(oTsprng/emale) vartatlom ('%)

pooled cortrol (%).

Control - I 29 35 32 27 32 31 31 32 32 32 100 31.3 6.7 Not appileable 10.98%

33 31 35 32 30 33 30 34 33 32 100 323 5.1

-3.2 22%

33 35 33 31 28 33 30 33 36 34 100 32.6 7.3 412 43.9%

34 32 37 33 33 32 32 37 32 36 100 33.8 6.2

-8.0 72%

34 33 34 35 32 32 32 36 35 37 100 34.0 5.2

-8.6 100%

36 34 37 40 30 32 37 40 36 37 100 35.9 8.9

-14.7 Control - 2 29 31 29 28 27 30 29 30 30 30 100 29.3 4.0 Not applieable 100% Intake 33 30 29 32 32 32 31

29.

30 33 100 31.1 4.9

-6.1 Outf2ll 101:

Dunnett's MSD value:

PMSD:

Intake:

Dunnett's MSD value PMSD:

2.300 7.3 1.050 3.6 MSD -

Minimum Significant Diffecrence PMSD -

Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatuent that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnla reproduction by 9.4% from the control.

Lower PMSD bound determined by USEPA (10' percentile) - 11%.

Upper PMSD bound determined by USEPA (90' percentile) - 37%.

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.

USIPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progm. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati. 011.

TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Statistical Analyses C.erWd.ph.

SorMv, %.d Rerod.edor T.t.Rpredscdi

.Sta Dte:

6/7/21X5 Test Mo CdFRR Somple W.

TVA/ Seqoyah NsckrPlant 101 - Non-trsted LnD DOe5: 6(14r.tD Lab M EMSEnvk.TestingSOL SampleType:

DINt Disec Msie MO iReport Simple Date:

Prnotocot FWCHR-EPA-Slt-R424013 Tert Speces:

CD.Ccxalpmi dubi Comment,:

C{nc.%

1 2

3 4

S 6

7 9

1t D-Cootrol 29.000 35.000 32000 27.00 3'000 31.000 31.000 32000 32000 310DD 10.91 33.000 31000 33.000 32000 30000 33.000 30.000 34.OO 33.000 32000 22 33.000 35.000 33.000 31.000 21.000 33.D 30.000 33.000 36.000 34.000 439 34.000 32000 37.000 33.000 33.000 321000 1000 37.0O 3200D 36.000 72 34.000 33.000 34.000 35.000 3'000 32.0 3'0D0 36.00 3 35.000 37.000 200 36.000 34.000 37D0 40.000 30.000 32000 37.000 4COOD 36000 37.000 Traisft Ustnsfrmn.d I-T&iltd JlmIk C.C.%

IMm NMs Mai Mb.

Mis CV%

N I&Stt telsl lSD M

N-.m D-CogkoI 31.300 1.000 31.300 27.00D 35.OD 6.744 10 33.317 I.OODD 10.9 32300 1.0319 32.300 30.000 35.000 5.0D6 10

.994 2.217 2.300 33317 1.0000 22 32600 I.0415 32.6C0 2S.000 36.0 7.259 10 19M3 2.2n 2.300 33317 1.0000 439 33.SOO 1.0799 33.100 32.00 37.000 6.206 10

.2406 2.27 2300 31.317 1.0000 72 34.000 1.0163 34.000 32.000 37.000 3.11I ID

.2.613 2.27 230D 33.317 1.00 100 35.90D 1.1470 35.900 30.0D 40.OOD t.l53 ID

.4.574 2.2t7 2.30D 33317 1.0000 AwsillrT Test Sbtaitic Ctidsl Sk.

Kurt rohoovDTest datesonmmaldmtutm(p>o

01) 0.741t21461 1.033 a.29761637 0.169024734 Bartlet's Tat Wind ta e1ad eunoes (p - 0.40) 5.1254 16756 15.0U627224 HythYsb Tet (I.6lO 9.0)

NOW LOKC ON Tu MSDv MSDj MSI 6tSE FV-Pr.&

O6 Dunreur Test 100

>100 I

2.299756327 0.073474643 25.97666667 3.057407407 6.3E-04 3.54 Treatments D-nontrol LrnSr Inastitt (200 Rnssnpl..)

Pl SD 95%CL Skn 105

>O00 100

>100 M102

>100 1.0 IC20

>100 0.1 IC23

>100 0.8 IC40

>100 C.7 IC0

>100 0.s Z 0.5 0.4 0.3 0.1 0.0

.. Is

°.. ° 0.2 0

20 40 50 so 100 120 Do. %

D".e4tep..se 7W 4: o 40 35 C 30 t25 10 0

10 5

iJ.

14all.0.06 hem oalgrlncame I

2 v.

sqn_06.07-05data

TVA I Sequoyah Nuclear Plant, Intake Non-treated June 07-14, 2005 Statistical Analyses Cer.d.pkas. Strvivi s"d "~rederit.. Tes.kprodcelm StartDstes 6A1?705 Tat WD:

CdFP9_R Samipkl M.'VA/

Suoh NUCIM IRIntake -Non-trst End Dste 6/14/205 Lab IDl.

ETSEnvk. TestingSol.

Sample Tps:

I)MR-Doda reMonkantg ptPCt Sample Du~e.

rot3owt FWCHR-.EPA.821-R0)2.0I3 Ta It SpNviff CDCuiodzpbni dubia Case.%

I 2

3 4

S 7

8 3 0 D-Co1okol 29000 31.000 29.ODO 23.0W 27.0W0 30000 290CtD MOD 30.0W0 30.000 too 33.000 30.0W 20000 32-.0D 32.000 32.000 3 1.0W 29.ODD 300WD 33.00W Came.%

M;..

N4-A.eu Ms.

Mia May CV%

14

%Stst Crd.'23 M.150 Naaa.

N.M...

D)-Control 29.300 3.M0 20.300 27.0WD 31t.0W 3.937 30 30.200 8.0000 IO0 31.100 3.014 33.30 Z?.0W0 330OW 4.900 3 0

.2.97 21734 3.050 30.200 3.0w AnTI~eryTests sted'tk Qrik.1 Skew IKsr1 iprowoeWs Tast k~d~tts nonnal diorkution (p >0.01) 0.921665691 0.066 4034337024.0.9042165 P-Tsstk d.3-e, utamvsr-=nc(p - 0*3) 1.727272749 6.541009533 Hypechls~s t

ag(114AU0.05)

MSDw PAS0p 065B 4S E F.Pr.&

dlf Homoefdasts: ITestidicates Do sjgnifiostdiffertnces 1.0500267 0.035337122 16.2 1.033333333 0.000156700 1,10 Dyeat6eota Vs 0)Coftrol Lness.. 0s3plsesd. (200 Ressampte)

FP41st

'A SD 95%C1 Skmw IC05

>100 IC15

>100 1.0 IC20

>300 0.8 1C40

>1000.

3C50

>100 0.7 3.6 30.3 0.2 0.1 0.0

.0.

0 20 40 60 so 100 120 Dose %

25 120 10 S

1-tall, O.0S level otstgficakflce I

8 MRn06.4-07.data

vnvironmental Testini! Solutions. Inc.

Page I of 6 1'nvirflnhllental Testing Solutions. Inc.

Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: TVA Facility: Seauovah Nuclear Plant NPDES #: TN 0026450 Project #:

IOgA~

County: Hamilton Treatment: UV-treated Outfall: 101 Dilution pre aration information:

Comments:

Dilution prep (N) 10.98 1 22 43.9 72 1 00 Each concentration was treated Effluent volume (mL) 274.5 550 1097.5 1800 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2225.5 1950 1402.5 700 0

to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 Interferences.

Test organism information:

Test information:

Organism age:

Z5 T j

-A.S oQauQS OQC Randomizing template:

ApLL &_

Date and times organisms A0.-&o-O5 vb3O -m Vto Incubator number: _

were born between:

Organism source:

PI&> RA~TA c--do-a Artemia lot number:

, > r Transfer bowl information:

pH Temperature =

'C Total drying time:

-AcAS

_53

.* C-Date I Time in: 0iti-OS IMI5 Average transfer volume:

IcO *o IQ Date / Time out: O'utSo-M i IScZA Oven temperature:

___C_

Dailyfeeding and renewal information:

Day Date Morning Afternoon Test Initiation, hlHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0

GS 0

ccr i358 0ioO C)SO;abAo*.0 tt

{ t I

Ino-ttcQs toC0 iS52-1Q6 1b2i t6-t4 Pt O-S.r1 4 0Z.;

A 2

6lo6-S Ocist i5SS IB0t C6E d cS C asoroa.L 14 '3 B i

3 C4-l&C5

/000 t§S57 Jq4t ci06M-0S C. cOooI.zckz.-L A W A

4 0b-Ik5S ngm3-

%15*0 13w20 cbd o6-S A, OSO" b.Gtz OB elk s

00 la-5

&S2.

VASoC 135Z-Ob-08 IS

o6 Lo -am 03 6

OCISO S

MS4 b 1 33;1 0&-OtO 6 OS0O.QZto 2

02

__..._13...

s Control information:

Acceptance critenia Summary of test endpoints:

% Mortality:

O s 20%

7-day LC50

> I W7f Average weight per initial larvae:

o.I1L, I

.; J NOEC IM7O Average weight per surviving larvae:

O.-11 2 025 mg/larvae LOEC

)100%

ChV IC2 5

lvironnental Testing Solutions, Inc.

Page 2 of 6 Species: Pimephalespromelas Date:

o0.-oi-OS Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data p

I' Ii I'

II II Day CONTROL 10.98%

22%

A B

C D

E F

G H

I J

K L

0

/0

/0 ID 10 10

/0 IC )

I0 lO 10 /0 010 0/0

_-10

/0 0/10 _

t/0 10 2

D1 10

/0 10 10 10 10 JO to io IC 10 3

I C) t010

/00 1 01 1( IC)/0

/0 /o 6

0 t

10 /0 IC 0 /0 /o 10 /Io /0 to 10 bo t

to0 'o to Jr A - Pan weight (mg) Ao--

Color Identification: EMWA tq8 t qo I4 Analyst: f* 66 I

,1. 1q 41 tC 1q4.90 i44. -s 1q.ri 'LAjs

,q.r'3 iN.li6Io 5.

(kiqL1 B - Pan + Larvae weight Analyst:,M

2. 2n.zl zz.zl zA2A 2+.50 z2.63 2z.i1 zt 22.15 z2.a2 zZAR 2I.qq Larvae weight (mg) - A - B

.22..I.SS

(.OD.1v jq0 go. l2.

-1.8& 1-10 1? 1..16.W 1.92-b6.R3 Weight per Initial number of larvae (mg) 4~-

k4 4,

6 C / Initial number of larvae Sc E

C'o n'0 Average Percent weight per reduction I

initial n

from control 0.:1l 1.gl3 Li1 LIO1S'-'

arvae me) of.

9 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.

Calculations and data rcviewcd: 4r Comments:

,,ental Testingy Solutions. Inc.

Page 3 of 6

,.nnta1 Testing Solutions. Inc.

Page 3 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: o to1-o5 Survival and Growth Data

.. -

I I

I D a y Day 43.9°/

72%

100%

M N

0 0

P Q

R S

T U

V W

X 0

'I iD-10 ID 01 10 IC0 1/

10 1

o1 o

/O /O

/O O l l

{

10

/

IC)b/OIO io)

//O

/

t2 ID l IC)

I*

_lo IO 10 0 lo IC) 3_

_t o, lbIto

(

I to 10 IC)

Io to IC) iI(

1t) 10 10 10 /0 10 10 1o0o to lV o 01010 I0.)IC IOt 6

1O lor

/~ _

to I Lr to I LW6 to t

to 10 I O 10 10 IA to I

/

0 10 to A - Pan weight (mg) 6"WI Colorldentificstion:

v5Mo~bi~Sq.3I.I 51i,.51.3,,9 4.l,.S1,d2 Analyst:

151 s

ll.1 qqS lqq5 l~l l...l,0 B - Pan + Larvae weight (mg)

(mg)

C

-Y Z-2 25 Th.-1M MIL 2Z.2lj \\

2Z.78 7>.z2 %z.'Z1 23-.z13 ZL22 Z3.1.2 2-z.(..

ZaZ4 Larvae weight (mg) - A - B 8.87 8-*

R.e S.o 1. 6S 7.77 R Ai1 1.16

&.-I

-1.l, 8.S;. -1v -.-27 Weight per Initial number of larvae (mg) 0 1

~A C I Initial number orlarvae O

O-O O

O Average Percent weight per reduction

.otI O7c 12 Initial from control 0.,i O.

0.Oq

-sq.

07

1. 8 l

_O51c number of

(%)

Iarvae (mg)_.

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - 1W-treated Date:

tb-C -S Survival and Growth Data Day 100% Intake Y

Z IAA BB o

0It I/O 1to 00 t

10 10_101 2

tO 10

/0

/

3 IC) lO 1

1 D

4 IC)

IC) I lIO 5

1 01

/I00

_0//0/

A Pan welght (mg) /5 Color Identification: 6 14.20 lq.10 15.l23 1.12-Analyst:

B - Pan + Larvae weight (me) 22 qO 23 13 232 Analyst: 41W_--A 214

.. 2 A2S 232Z, Larvae weight (mg) - A - B 1.60 8AB1 9.07 L gsk Weight per Initial number of larvae (mg)

V O'V C / Initlal number of larvae

\\0 Average Percent weight per reduction Initial from control O.8 number of

(%)

larvae (mg).

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:&

Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated June 07-14, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephatespromelas Quality Control VerifiCation Ot Data Entry, CalculationS and Statistical Analyses alkmt:

Test dates:

TVA /Sequooyh Nulenul antOutfal 101. UV treated lune07.14,2005 Rcelw ed by:

-I Project aumber.

2 It" Not for Compliance Asessment. [ntesnal.aborlory QC t(m'.

t%3sl.8.

41 FII~w nl

8.

rhe A -

ft.

.dzhI

-ts 8.

tL, t nl*e m1

()

Wsi:8 I8ntd

~

65.

.MIR C

_d.4 -vl-.

wuh Is I4e3 lu8 t t -w*s r

Fh St.

dgbd C..d.t.

hron

.Wa.

_ s 9l iilu A5

@edvfy ett bsfl 1

lb~

vlr_

t ta t a*

t)h (A) bnw"A-t d

I__

A I 0 t0 34.98 23.20 8.22 0.822 0.822 Control B

20 to 14.69 22.21 755 0.750 0.776 4.0 0.7t2 100.0 0.776 4.0 Not applicable C

30 20 14.61 22.21 7.60 0.760 7.76 sD to 10 14.80 22.48 7.6t 0.76t 0.765 E

to to 14.90 24B0 9.40 0.940 0.940 10.9t%

F 10 tO 14.51 22.63 7.12 0.72 0.8t13 1 3.8 0.725 100.0 0.513 11.8

-t47 G

I30 30 24.89 22.77 7.88 0.788 0.728

___3 30 3 0 14.98 22.08 7.10 0.710 0.710 3

10 3 0 14.83 22.75 7.92 0.792 0.792 22%

K 3 0 30 14.70 2232 7.62 0.762 0.755 6.5 0.762 I00.0 0.755 6.5 2.8 K

I0 I30 135.16 22.98 7.82 0.782 0.782 L

30 20 14.66 21.49 6.83 0.683 0.683 M

10 9

15.16 23.98 8.82 0.980 0.882 43.9%

N t0 to 4.72 23.71 8t.9 0.99 0.874 10.2 0So 97.5 0.849 7.0

-9.4 0

3 0 3 0 14.73 23.23 8.50 0.850 0.850

_p I30 2 0 15.16 22.81 7.65 0.765 0.765 30 S0 I5.01 22.78 7.7?

0.777 0.777 72%

30 30 14.75 23.22 8.47 0.847 0.8tO98.2

.O847 I00.0 0J09 8.3

-4.2 S

30 30 14.93 22.27 734 0.734 0.734 T

t0 30 14.95 23.73 8.78 0.878 0.87t U

t0 30 14.61 2218 7.67 0.767 0.767 Il%

V tO 20 t4.66 2322 S.66 0.866 0.781 7.8 0866 300.0 0.781 7.8

-0.5 W

30 30 35.00 22.67 7.67 0.767 0.767 X

t0 t0 1522 22.44 712 0.722 0.722 30 34.80 22.40 7.60 0.760 0.760 Io-r. Intsae Z

30 20 24.70 23.13 8.43 0 843 0.839 7.0 0.942 300.0 0.839 7.0

.8.3 A_

30 30 5.723 2425 9.02 0.92 O.9S2 all0 30 30 34.72 23.23 8.53I 0.851 0.853 Outrall 01 Dusustels MSD value:

rMSD:

Intake:

Dunnrtl's MSD value PhtSD:

0.e079 33.9 0.0646 83 MSD -

Miniamumn Significant Difference PMSD -

Percent Minimunm Significant Difference PMSD Is a measure oftest precision. The PMSD is the min~lmm percent difterence between the control and treatnent that can be deelared statlstically signifieant n a whole effluent toxicity test. On averige. a significant difference occurs tor Environmental Testing Solutions. Ine. chronic toxicity tests vhen a toxicant seduces PFmephatte growth by 14.0% ons the control (determined through ecteeence toxicant testing).

rwer PMSD bound determined byUSEPA (Ihth percentile) - 9.4%.

Upper PMSD bowod determined by USEPA (90th percentile) - 35%.

The lower uad upper bounds were calculated by the USEPA usin g 205 tests conducted tono 19 laboratoriet fore RFepksfes growth in chronIC teftrence toxicant tests.

USEPA. 2000. Undestundingand Accounting for Method Vaiabiy a Whole Emunit ToxciyAppGcatos Undfthe National Polutant Discharge Elmiuatio Prognm. TEPA-833-R00003. US EnvironmentaI Potection Agency.Cincnat. 011.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated June 07-14, 2005 Statistical Analyses LArmFb Cra.

sad SurvIal Teut.7 DayCrwwt S3ta Dat:

6tnos Test ID.

ppRCR Sample ID:

TVA/ Sequoyth Nuclear Plent 101 - LNVdeated Eod DaL:

E14=5 Lab lD EFE. TaS6g sol Sample 7yc:

DI)MR-Dichare Mfaltodft RPood Sample Dbt:

Protocol FWCHR. EPA-21-R424-13 Test specl:

pr-.Fmerphale promela C

.Y.

I 1

3 4

DQConfrol 0.22D 01750 0.7600 0.760 10.93 0940 0.3120 0.71130 0.1200 22 07920 0.7620 0.7120 0.6t30 43.9 0.1120 099 0.1500 07530 72 0.770 0.470 0.7)40 01730 100 0.7670 0.3660 0.7670 0r.20 Tranifrm: Uat

-farvaom I3Tailed I,4.-le C.e.%

Me-.

N-Mea.

Ma.

Mh Me l

CVY.

N

-Stt Ctltket MSD Me.

t-;4tl D.Cou

&7763 1.O00 0.7763

.7550 n.220 3.939 4

0.303M 1OCOC J5093 0.3123 1.0467 0l7.35 07100 QS400 11.754 4

.0.t10 2410 0.079 0.ntoo3 I0O 22 0.7543 ar9723 0.7348 630 0.7920 6.550 4

04,10 2.410 0.3079 0.$303 3.ox 43.9 0.8490 1.0937 0.890 0.7650 o.m0 7.017 4

.1.625 2430 0.3079 0.30X3 1.ODO 7n o.3090

.04 0.090 0.7340 0.m70 3.091 4

nn71 2.410 0.2079 0.303 1.OXD 100 (15

.0033 0.7903 0.7220

.t660 7.792 4

4095 2.410 m.079 0.7CS 0.9753 AdyTt Stetle cOt.l Skew Kurt Sbapwk*.Wr Tadt data normal daVmtNa (p > 0.01) 0.93221791I ntu4 0.n34096212.0.0596U54 Bufftg Teat kdlatta equa varinces (p -0 65) 3.292W4too 15 0S677224 kly(ptealu T

eat (I.&II, 0.03)

NOEC LOW OV TU MSD MSDp MSB MSE FY.a C

DuatsaTeat 100

>100 I

ot07903775 o0139(06473 0.0044609 0.004009306 0383406605 5.LB atment Ye D-04eCol Lheor lutaala(200 Ittoo aanples)

PW.

SD 95%.CKep)

Stew ICOS

>100 ICIO

>100 ICIS

>100 1.0 C20 OD 0.9 1023

>100

.0.3 IC40

>100 0.7 2030

>300 0.6 0.5 0.2 0.2 0..

.0.1

.0.2 a

20 40 60 s0 100 120 D

Dw"Rw"*eP.W 0.3 0.7 C.?

I0.6.

es O 0 0 0.4 VW 0.3 0.2 0.1 14al. 0.05 level d sgriflcarnce 9:

6 p

N sqnO607.OSdata

Environmental Testing Solutions, Inc.

Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalesprotnelas Daily Chemical Analyses Client:

Test dates:

TVA / Sequoyah Nuclear Plant, Non-treated June 01-14, 2005 Project number. 199 Reiwdb-,'

Y I

Concentration I Parameter Day0 ntal Dav 2 Dav 3 l

Dav 4 l

DayvS Day 6 4

4 Initla I _lnal

.4-

.4 Inithl

,1II (Sill DO (mZ/L) 2onducetvitv (umhonen)

Initial I

Final I Initial I

Final l

7.851 7.671 7.721 7.701 7.8 7.4 7.8 7.9 308 2 94 58

_82 InitIal l FInal Control initial I hi s is r 4-heI z

Alkalinity (mr/L CaCOA) 4-Ilardness (mg/L CaCO,)

7.651 7.532 7.68 7.42 7.8 7.5 7.6 7.5 303 297_

62_

80_

24.8 24.6 24.8 24.5 7.84 7.60 7.73 7.43 8.0 7.3 7.6 7.6 290 276i Temperature (C) l

.4.YI Z4.YI 4a.UI l -

4--

All (SU) 4-3O (m/DL) 7.89 7.75 7.97 7.61

.8.11 7.41 8.1 7.51.

287Jiu1 285 Ji~

10.9R%

-onductfvftv (umho:

ml Codutiit (im4-¢m I

_Temperature(C) 25.3 24.6 25.01 24.3 24.81 25.1 25.1 25.11 25.1 24.6 249 24.5 24.7 24.5 pil (StJ) 7.88 7.66 7.83 7.59 7.87 7.78 7.96 7.621 7.77 7.68 7.84 7.61 7.72 7.45 22%

DO(mg/L) 7.6 7.8 8.0 7.3 8.1 7.3 8.1 8.2

7.

80 7-4 7.6 7.7 Conductivity pmhos/cm) 273 276

_27 274 271 28t0 268 Temperature (C) 25.2 24.6 25.0 24.5 24.9 24.8 25.1 25.1 25.0 24.7 25.1 24.3 24.7 24.5 piI (SU) 7.85 7.64 7.80 7.60 7.83 7.83 7.95 7.62 7.74 7.68 7.83 7.63 7.71 7.49 43.9%

DO (mg/L) 7.6 7.5 8.0 7.3 8.2 7.4 8.0 7.4 8.2 7.8 8.0 7.3 7.7 79 ConductivIty (umhos/em) 248 255 2521 253 248 258 245 Temperature (C) 25.2 24.8 25.11 24.4 24.9 24.8 25.0 24.9 25.0 24.7 25.1 24.5 24.8 24.5 PI(SU) 7.1j 23e 7.69 7.80 7.64 7.81 7.85 7.92 7.63 7.70 7.71 7.81 7.61 7.70 7.50 7t%

DO (mg/L) 7.6 7.7 8.0 7.3 8.1 7.4 8.0 7.5 8.4 7.7 8.0 7.4 8.0 8.3 Conductivity (,mhostcm) 227 228

_ 1 222 223 220 226 211 Temperature (C) 25.0 24.6 25.0 24.5 24.7 24.8 25.2 25.1 24.9 24.6 25.1 24.4 24.7 24.3 PI. (SU) 7.78 7.68 7.79 7.651 7.79 7.83 7.91 7.63 7.65 7.74 7.78 7.641 7.69 7.57 DO (mWL) 7.6 7.81 8.0 7.4 8.2 7.3 7.9 7.6 8.2 7.7 8.0 7.6 7.8 8.3 Conductivity (jmhoslcm) 197 202 194 192 189 19!

183 100%

Alkalinity (mgfL CaCO)7 71 71 I ardness (mg/L CaCO,)

80 80C Total Residual Chlorine (mgfL)

<0.10

.10

<O.I0 Temperiture CC) 25.3 24.7 25.0 24.5 24.7 25.0 25.21 24.9 24.9 24.7 25.1 24.4 24.7 24.4 p11 (SU) 7.90 7.66 7.85 7.68 7.87 7.89 7.95 7.68 7.88 7.74 7.84 7.67 7.70 7.57 DO (mgrL) 7.6 7.8 8.1 7.3 8.2 7.5 7.9 7.5 8.5 7.8 8.1 7.6 8.0 8.3 Conductivity (mhoslcm) 196 201 194 192 189 19' 180 100% Intake Alkalinity (mgtL CaCO,)

74 75 73 llardness (m/L CaCO) 88 71 78 Total Residual Chlorine (mg/L)

<0.10

<0.10 Temperature (C) 25.3 24.61 24.8 24.5 24.9 24.8 25.2 24.9 25.0 24.5 25.21 24.5 24.9 24.4

Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated flitkv Chemistr":

Date:

O%.-

- G5'S Dav

.0 I2 Analyst Concentration Parameter AW WlGi CONTROL pH (S.U.)

es_____

_1_-U1 DO(mg/L)

,<1

).6 8.O Conductivity 500S (gmhos/cm)

Alkalinity (mg CaCO/L) 101 Hardness (mg CaCO1/L)A Temperature(°C) 2 s-Z..'4S zt. 8 21.l b.4.

A pH (S.U.)

+.t IaCl 10.98%

DO (mglL)

_1___ _1_

Conductivity 2'5e (Amhos/cm) 2 2q 0

Temperature (C) 2S.B zq.1 7

-24.

Z1 2S pH (S.U.)

IOS.e?

l 22%

DO (mglL)

_4AC n

01

-7 a-Conductivity Z43

?.to Temperature (C)

ZSZ mg.lo 25.0

l.

4's.5

~.q l

.8 pH (S.U.)

_A

.Eo.

3-8 3 2 03 43.9%

DO (mgtL)

_I.0 1 -7 Conductivity zq_

1(nimhostcm)_____

Temperature (C)

S';Lq Z 2'Z*

1i(.

l

.4.

pH (S.U.)

72%

DO (mg/L)

_p _

A.0

_.5 Conductivity Z

(pmhos/cm)

IZ 3 +

-2Z&

Temperature (°C)

IS.0 pH (S.U.)

100%

DO (mglL)

O Conductivity (ymhostcm)

Alkalinity (mg CaCOtL) 1t Hardness 4

(mg CaCOL)

SC)

TR chlorine

'-0.10 Temperature (SC)

s. -

pH (S.U.)

4O I

100% Intake DO (mg/L) 4;(a Conductivity 1

(grmhos/cm) 19° Alkalinity (mg CaCOA/L) 7 Hardness (mg CaCOAL)

TR chlorine (mg/L)

(0C Temperature (°C)

ZkS-.3

_-4. -+

1 4,G.7

nt LI 29n S

,_- : ).s

-4.0 is I

I e40.10 Initial I

Flnal I

Initial I

Final IInitial P

Final Initial Final I Initial I

Final I

Initial

F; viro nmental Testing Solutions, Inc.

Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plai Page6of6 nt - Non-treated Date:

tM-dC-OS Day A

S 6

I.II Ii I

Analyst KC-,

Ib~

V)PN _

XKsAI AC it Concen-Parameter CO.NTROL pH (S.U.)

_. 4 7.10.

5

.8 DO (mg/L)

-7..

".X 7

7 Conductivity zoo q t3 (mg CaCO1/L)

Hardness (mg CaCO3/L)

Temperature (C)

V A

.°l

___.D Z. P*____8 pH (S.U.)

3 2CC

7. f_

c O

_.-I 10.98%

-DO (mg/L) 0.1 9 5 010 7-3

7. L-7 C Conductivity 2835 22 q

27

.(pmhos/cm)

_ 5_______

Temperature (°C)

25.

Z.

S 7I Is 71 i

.5 pH (S.U.)

4.91 4AO7 6

L 1.6t 4.l__

22%

DO (mg/L)

0. I Conductivity qq

( nimhos/cm)__

Temperature CC) 35j 5-1 LS.Q

-j L'.

R4.1

?A.$.

pH (S.U.)

-7

- 6

1. jX 1.1.3 1.V Z

43.9%

DO (mg/L) 6A.

S

8. 0 7.

Conductivity ZS3 2Sd (TMhos/cm)

Z5

____Temperature (C)

I

.O 2'A qSQ j

-ZS V4.

A8 pH (S.U.)

-40i2

-7.11 1.61

-1.1r) 72%

DO (mg/L) 8

'i.S

a.

-7. S 0

7 4 O

3 Conductivity Z Z3 I

22zo 1

2ZILD V)/ /

(pim hos/cmn)

I__

I___

Temnerature (°C) 25.7-

-is.

I-.

L -3 4

4 ---

0

§7 = 4

41 I.

41

4 41 I'

pH (S.U.)

-4.*O1

-7.7+

1-. L

71. V 444. Slit 100*/0 DO (mg/L)

-.1

~.

I

-_._t Conductivity (Amhos/cm)

lc0, Alkalinity (mg CaCO/L)

L 1[ I 1 Hardness (mg CaCO/L) 8 TR Chlorine (mglL)

C O

r..

Temperature (C)

Z2S 2.4.¶ 4

7 ZS. I Z4A Z.-1 24.Z(

pH(S.U.)

t.9

.D 4 I.7 7-8 1

i

.10.1 100%

DO (mg/L) gf 7*4 Intake C)__

7S'.'

Conductivity 19 z1 80 rnmhoslcm)ec Alkalinity (mg CaCO/L) 13M Hardness MARw (mg CaCO,/U TR cblorine (mglL)

C)O

. I O Temperature (C) 2S 2Y.9 ZS 7_

l7 Initial II Final I

Initial II Final Initial I

Final I

Initial I

Final I

S -

5 6

U -

S -

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphitia dubia Daily Chemical Analyses TVA / Sequoyah Nuclear Plant, Non-treated Clitnt:

Test dates:

June 07-14, 2005 Project number: 18"9 Concentration Parameter Day 0

Initial l

Control pIH (SU)

DO (mrJL)

Conductivity (pmhostem)

Alkalinity (meIL CaCO) 4-Hardness (mg/L. CaCO3) remnerature (C) nil ISI I 81 4-DO (mg/L) 8.21 8.11 8I I R8 8.

7.

8.0 o.,,

10.98%

i

.. __. wm miv-

~~w~~i

__L~~

=II-Conductivity (umhnsteml I.

\\ w -

Temperature (C) 24.61 25.21 24.8 I 25.0 24.61 25.1I 24.

25.:

24.51 24.

24.

25.

24.

25.1 pit (SU) 7.88 7.73 7.83 7.77 7.87 7.89 7.96 7.781 7.77 7.77 7.84 7.66 7.72 7.6i 22%

DO(mg/L) 7.6 7.8 8.0 8.0 8.1 80 8.1 82 8.2 8.0 8.0 8.0 7.6 8.1 Conductivity (pmhoitkm) 273 276 277 274 271 280 268 Temperature (OC) 24.7 25.2 24.8 25.0 24.6 25.1 24.9 25.1 24.7 24.8 24.7 25.2 24.8 25.I pi (SU) 7.85 7.74 7.80 7.74 7.83 7.89 7.95 7.79 7.74 7.77 7.83 7.66 7.71 76 DO (nJL) 7.6 7.9 8.0 8.0 8.2 8.0 8.0 8.2 8.2 8.0 8.0 8.1 7.7 B."

Conductivity (Gimhos/cm) 248 255

'22 253 248 258 245

_Temperature (C) 24.8 25.0 24.8 24.9 24.7 25.2 25.1 25.2 24.7 25.0 24.9 25.2 24.9 25.1 nit IqII1 7_831 7-7 1893(

7.74 7.8II 7-9tJII 7.921 7.79 7.701 7.7 7.XIi 7.6' 7.7(

7.6 72%

30 (mg/L) 7.6 7.91 8VA 7.9 8.;

7.

8.0 8.i1 8.4 8.C0 8.01 8.2 8.0 8.

Conductivity (umhos/cm) 1 227 _m 221 2 22 14 223 II 220 226 211 Tempernture (Co pll (SU)

DO (mnWL)

Conductivity (pmhos/cm) 100%

Alkalinity (mg/L CaCO3)

I lardness (mg/L CaCO,)

tat Residual Chlorine (mg/L)

<I Temperature (C)

I 7.76 7.79 7.79 7.79 8.0 8.0 8.0 8.2 20;194 80

_11<0.1(0I 25.11 24.812S.3124.91 7.79 7.65 7.79 8.2 8.2 8.0 189.

71 80

<0.10 25.2 25.1 25.0 7.81 7.88 7.79 8.0 8.5 8.0 189.

73 78

<0.10 25.2 24.7 24.8 pil (SU)

DO (mEgt.)

Conductivity (umhos/cm) 100% Intake Alkalinity (nV)L CaCO,)

-7.61 7.91 8.1.1 8.21 7.9_

196 201 194 74 75 88 7

24.10

<0.10 24.81 25.11 25.0 2.

11ardness (mgtL CaCO,)

4.-

Total Residual Chlorine (me/L1

\\

w Temperature (C)

Environmental TestigSltons, Inc.

Page 6 of 7 Environmental Testing Solutions, Inc.

Page 6 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Daily Chemistry:

Date:

C1b-01-OS_

Dav Anayt Concentration Parameter CONTROL pH (S.U.)

___1__

DO (mg/L)

+

L9 w

Bl IaO w

Conductivity Nimhos/cm)

Alkalinity (me CaCOL) to3 Hardness (mg CaCOt5L)

Temperature (C) 1.L. 2.1 A 1

2.

pH (S.U.)

3;-4 4.8q 10.98%

DO (m/iL)

I

.6 Conductivity (Amhos/cm)

Temperature (C) 2.I q. e r;7 L

.~

H (S.U.) '

22%

DO (mg/L) d i

Conductivity

(

(;imhos/cm) 5.

Temperature (C) 2W.1

_4._4' pH (S.U.)

43.9%

DO (mglL)

Theo Conductivity (umhostcm)

Temperature (C) 2L40 Om 7Z7 ZW 1

L.

12Yg-E 0

IV-1 -(.

r T F w.S 4.0e:, *.

i to

_o 6.s l

55--5 1_ ScD

_4-0

-40 7e1

.,o (3q,1

.q, I

wze 22z\\.i ~

IP 7-S n-qs~

IL4.J pH (S.U.)

72%

DO (maL)

Conductivity (umhos/cm)

'2ZI x,

~~...

Temnerature MC) pH (S.U.)

+.3

100%

DO (mglL)

.IO Conductivity (pmhos/cm)

Pq Alkalinity

-14 (mg CaCO3JL)

Hardness

&O (mg CaCO3/L)

TR chlorine

)O 1 Temperature °CC)

IZi.

I Oz T

1 6z. I DH (S.U.)

4.- -4.9 1 :t.-iq

_-A I_4.

100% Intake DO (mgL)

-7 1

.80)l

-C 0-to

=:

-' -5 4 2 s

?eq r H iRP5

-it E

4010 Sg Conductivity (pimhos/cm)

I 9 t Alkalinity

-7q (mg CaCOIL)

Hardness (mg CaCOJIL) o TR chlorine (mgIL) 0 0

-I--___

201 Temperature (°C) 2 4. &

I I

I2.

1 24.1 I

Zs. iI

..-

initila i Final I

i t F

Final Initiai Final I

E~nvironmental Testing Solutions, Inc.

Page 7 of 7 Environmental Testing Solutions, Inc.

Page 7 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date:

06-61-OS Dsv 4

%5 6-

Analvst ik-I siy 19 14J-1 li_

I 4:YW Y A I

AA 11f I

Cooce n w Parameter tratlono_

CONTROL

-.4_PV q-1?

1 7.-7'1 1.65 I

1.X7 ii I

DO (mgJL) n - I I

I I

Conductivity (prnhos/cmn) e Alkalinity (mg CaCOI/L) -

l S 8 Hardness (mg CaCOAL) a 28 SD g o Temperature (°C) 25.

I Z.1.

2s.3 7zL.- I t

I nH (S.U.)

-1.?

10.98%

DO (mg/L)

It I

.2 Conductivity I

(gpmhos/cm)

I-5 Ia7. 4 1A.-!a

. 6

_ 7s 7AW

-53 IS. 6 Temperature (°C)

ZM.9 25.3

-)-%A

.-s I

I*

9 -r--

9

0-pH (S.U.)

-I.S(Ao 1.12-.

22%

DO (m!/Ll A 1 4.0

-l0 Conductivity

-I 7

Zi E

2 20 (Prnboslctn1)

I- -_TI Temperature (°C) 2-4.M

--.-I pH (S.U.) *.

C1 1h :

.Li

7. It :*

43.9%

-DO (mg/L)

I L2 B..

(umhos/cm)

ZS3 243

_E_2Y Temperature (°C)

Zs. I 2S

-7A.

25.0 3

2j pH (S.U.)

3.9q 72%

DO (mv/L) a-,

_wl u

Conductivity (umhos/crn)

..2ZO Temperature (°C) 24-.

0 pH (S.U.)

r--

o 100%

DO (mg/L)

I v

Conductivity (umhos/cm) x Alkalinity (mg CaCO,/L)

1 0,2-

.0 Q.SD I

I_

109 1i q I5,.

40.10

_~~~~~V

5.

Z.

RE;) 759 1

qq 5.

Hardness (me CaCOJIL) x _ _ _ _

TR Chlorine (mi Temperature (MC

?IL) pH (S.U.)

DO (mg/L)

-:~q I0.-0 100%

Intake Conductivity (Pmhos.cm)

Alkalinity (mg CaCOJ/L) 0.5 g.,a 1 9

.13 I &9 m!

l 7

Il9 l&o 1 P.oI Hardness (mg CaCOJ/L)

TR chlorlne (mg/L.)

Temnperature (°C)

75.

U 27.TL

---

4-1 S,. 0 CZ -. t'

-. Z I Initlal I

Flnal I

Initial I I Initial I

Final I

Initlal I

Final I

initial I

Final I

InitIal I

Final I Initial I

Final I

Initial

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalespromelas Daily Chemical Analyses TVA/Sequoyah NUCear Plant, 1W-Treated Client:

Test dates:

June 07.14, 2005 Project number:

1899 Reviewedby;_

Concentration Parameter l)a 0 Day I Va 2 Da 3 Day 4 Day 5 V

6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pit (SU) 7.86 7.64 7.76 7.63 7.80 7.73 7.90 7.57 7.81 7.74 7.82 7.53 7.68 7.50 Control DO (mzJL) 7.7 7.7 7.9 7.5 8.2 7.2 7.8 7.3 8.1 7.9 8.2 7.1 7.7 7.4 Conductivity (pmho-Jkm) 2430.9 299.2.86 295 2196

________Temperature (OC) 25.2 24.7 24.9 24.8 24.9 25.1 25.0 24.9 25.1 24.6 24.7 24.3.

24.8 24.4 pit (SU) 7.86 7.64 7.76 7.65 7.79 7.71 7.91 7.57 7.79 7.70 7.82 7.60 7.71 75 109% DO (mzfL) 7.6 77 8.2 7.5 8.2 7.1 7.7 7.3 B.1.

7.9 8.21 7.2 7.77.

109% Conductivity (pmhios/cm) 28'4 293 288 28'4 282 292 2'

________Temperature

("C) 252

2.

25.2 24.6 48 24 24.9 24.9 25.0

2.

24.91 24.6 25.0 2.

pi1 (SU) 7.85 7.4 7.76 7.4 7.79 7.78 7.91 7.8 7.76 7.5 7.81 7.59 7.71 75 22%

DO (mzfL) 7.7 78 8.1 75 8.2 74 7.9 728.1 788.3 7.2 7.672 Conductivity (pmhoslcm)25 282 276 27/5 272 28174

________Temperature

('C) 25.3 24.6 25.21 24.6 24.8 2

25.1 25.0 25.0 462524.

25.0 2.

nil (SU) 7.85 7.63 7.76 7.61 7.79 77 7.91 7.60 7.74 7.69 7.81 7.8 7.71 75 49% DO (zL) 7.6 7.5 8.1 7.4 8.1 73 7.8 7.5 8.2 7.8 8.3 737.874 Conductivity (pmlioslcm) 263 261 253 253 249 29251 Temperature (C) 25.4 24.9 25.1 24-9 24.8 251 22 24.9 25.2 24.6 25.2 24.31 24

24.

3 pH (SU) 7.84 7 62 7.77 7.67 7.77 775 91 7.57 7.72 7.68 7.80 7.60 7.71 75 72%

DO (mz/L) 7.7 7.7 8.2 74 81 71 78 7.4 8.2 7.8 8.4 7.2 78 7

Conductivity fpmnhos/cm) 226 232 22625 221

~

227 221 Temersture(C 25.2 24 25.2 248 29 251 23 25.1 2501 24.4 25.1 24.4 24.9 24 PI[ (SU) 7.82 77 7.75 769 75 7.7 791 7.61 7.68 7.76 7.79 7.61 7.71 7.50 10% DO (mgIL) 7.7 7

8.2 74 82 7.1 9

7.3 8.1 7.8 8.3 7.4 7.9 7.4 Co0%nductivity (tzmhosjcm) 16208 197 194 196 1(.I9 Tmperxture CC) 25.2 24.8 25.1 2481 24.9 251 22 25.3 25.1 24.7 25.2 24 25.1 24.5 pit (SU) 7.90 7.64 7.77 7.651 7.79 783 93 7.62 7.80 7711 7.82 76 7.73 7.51 100% Intake DO (mnL) 7.8 7.7 8.2 7.31 831 751 8.0 7.3 8.2 781 8.4 74 2 3

Conductivity (_umhoskcm) 198

~

2?!F 195 PEI"191 189 194 11

_______Temperature (9C) 25.3 24.8 25.1 2481 2481 25.11 25.4 25.1 25.11 2471 25.3 242501 2415

al Testing Solutions, Inc. e SPage 5 of 6 A'

Species: Pimephalespromelas

...... Client: TVA / Sequoyah Nuclear Plant - UV-treated

.fl%

Date:

06 OS DailY Chemistrp:

-"--I' t.*,

.'-

.

I i.

I.

I I

Day 0

1 2

Concentration Parameter CONTROL pH (S.U.)

4_ 30 l

eo S

DO (mg/L)

)

1 5

1ttT7 Conductivity zq0 (p +/-m hos/c m)_

Temperature (C) 7S.2.

I l

ts.%

p1l (S.U.)

J 77G3 39

?

10.98%

DO (mg/L)

.to

.0 1.2 Conductivity z&e4 (RTmheslcmt) in7__2_

Temperature (C)

'ZS.2. 3

.1 2Sb

?-1.(o ZS 7.l pH (S.U.)

__-___1__

24%

DO (mgfL) 15l2,1 Conductivity Temperature CC)

-Zs.

2.t

2. 1 2

ZL a

-Zs.l pH (S.U-)

4403

--II-n-to S-4Du i t.Tq Fy 43.9%

DO (mgfL) 13.?

ell X.

/

Conductivity 1(5212

.(gmhos/cm) 2Zt 3

Temperature (C) 2S.Z 2'4-4.

25.1

'R 2I.2 1.S.1 pH (S.U.)

2 it 5Ak?

5 R

72%

DO (mg/L)

Conductivity 2

(umhoslcm) tq

-DZof Temperature (C)

Z Z

E.

9 I.

'Z 9 1

100%

DO (mgi/L)

_.3 7;

Conductivity q

0 14 (iunhom uslcimi) 19~0 2t___ 1_ __

_l ;

Temperature ( 0C) 3 I

Ij S

q9 nj l

C oiti altF i n al Initial Final Initial Final Initial Final

I restinty Solutions. Inc.

Page 6of 6 Testing Solutions, Inc.

Page6of6

! Species: Pimephalespromelas

. Client: TVA I Sequoyah Nuclear Plant - UV-treated i,. ;;.:-

Date:

06-01-gL 0-

!.

-,, -Z Day I

.h t

i I

F t

¶1

I I

Analyst 1~tk I

I1i-tjnq. A --

Parameter I

nUT (S-U.)

4 or

-7.74.

. LOil'KiL e'sk i-

1.....,

DO (mrgL)

I 3S. )

j

.9

/

7 7.7.7 ConductIvity I

2ex.0 I ____

i28 )..-

Temperature (°C) 24.9 2-S.I Z%4.i

.-J I -Z4.-

.zq.q 1-14.

' 22%

S.;',..

pH (S.U.)

-.10

.to0 I -1.-I DO (mgiL)

.J l

1 1

1

8 t

a 7.7 1

Conductivity (p+/-mhoslcm)

.1'

. M 2aZ hL~ 1

-J lcpi>

Temoerature IC) 7-4.

Lw.L Zs.

pH (S.U.)

-4.?tw

7. 45

17. b' D0 (m-/L)

-'ii9 Conductivity Z-1

_____s I

i I

P 14 Ttmperature (°C)

-LS I lS.° Ls.0 25.0 Y

F I! -Y

-.

ii P

.S0 1,5

!I

. 43.9%

;I -:

I5, pH (S.U.)

-7."'

-.l5W 9.- I DO (mglL) ij 4

V 8

i (gmhos/cm) 7265 2Lfq ZE___

Temperature (°C) 24.q 2'I.3 I-I

_*~~--

A g'~'-

4

_~~

I?

4_

': 72%

II..

pH (S.U.)

1.69

,AaO

'.- I DO (mgL)

q. J; nf - -

- -J aI--,,--.

Conductivity I Z 51~2

~

~J (gimhos/cmn)

I L2 WJ 0z ifl 2z.

O IcyP'QI Temperature (°C)

-S.0 2Z.4 ZS I 2L3 °l pH (S.U.)

-4.9 I

-7. 1 i

-.1 t 1k _7.

100%

DO(mg/L)

.cm9 41 1.3 0.1 i.-

3 Conductivity i1-4 yr qw r, o9 ____

/, L J

_lmhoslcm

) -

.S.Z -

S 7

r Tempoerature (°C)

- 2S Z.

_Z-7-St 4

ZS. -t a

ist 2. I Zzq.s pH (S.U.)

1.11

-1.La' I I * -10n 3

100%

Intake DO (mg/L) 8-0

Z..

3 B.Z i-1.Y 7 +

7. 3 Conductivity 1 c 8.1 i i Temner2turp (°C'l 12S 1

7q. t

,dI 5

1 2"c nX 9

O.- -I

--

0 9-,-§ -

I---

Initial Final Initial Final Initial Final I Initial Final

. n S -

a

Environmental Testing Solutions. Inc.

Page 4_ _

Page_ Lof I

Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0. 10 mg/L Meter Accumet Model AR25 pH/Ion Meter Analyst I ki-Y I l1 Date analyzed I Db.0-4-o5 Iodidereagent: lIjiR l

q Acid reagent: l I

ba Calibration:

i-i

.1U0 m /L l

1.00 m=/

! Reference standard number I IW 2

I t4SSaO I

Note: For samples with a residual chlorine of > 1.0 mg'L the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (NIV)

% RS = MV /TV x 100 number (mgIL) t(meL)

(acceptable range - 90 to 110%)

/

IT )

0.50 O 614b lQE5 1

Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD - U(S - D) I[(S+D)121) x 100 number (acceptable range - *10%)

l C0S5bW., Oil

_C,

- OD

-b,d-S /-O 06Zq I ft rL Duplicate D

U 33l l

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine

number (mg/L)

Blank (shouldbix<D.10mgrL)

A O. q 1'

"0 2.

coC*o1.O2..

fzc 10 OOC.QOg., C.AiA oSoI-1,c3 Oorv A

P 4,

p-

-c-O UZ 9Z osbsa o0q Q,;tvb ahkU-w tno L10 Ctlle 4-COO %09C£ 0

06LOpb-oL 1A-StokJ foI g"

, ckaa O.O O5aco.o I

_o

=oc, C

lUWCUXc Lo.VOZpgo obto~to.D FF f-)I pi

-a(Z 05 4y

,~

C1 4L 1u hd It01o Note: All samples were analyzed in excess of EPA recomnmended holding time (5I minutes) unless otherwise noted.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS MV /TV X 100 number (mg/L)

(mg/L)

(acceptable range - 90 to 110%)

S2 U0.50 a

s l

01 Reviewed by Date reviewed

LM.

Testing Solutions, Inc.

Page 6t Page L of I Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, hlDL= 0.1 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst

-a 7 Date analyzed O toC&Oi s

Iodide reagent: l I C 1 Acidreagent: I wa vSk.

I7 F..

Calibration:

l 't-ki.2*t.

0.10 mz/L I

I.00m~JL I

Reference standard number l

C

)IltS2 l

Z1 0

I Note: For samples with a residual chlorine of > 1.0 ing1L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control staufdard:

Reference standard True value (TV)

Measured value (NIV)

% RS - INIV / TV x 100.

number (mgfL)

(m&/L)

(acceptable range - 90 to 110%)

WIGi S

.1*

0.50 o.'1T1 Duplicate sample precision:

Sample Sample ID Sample characteristics Residual chlorine

%RPD - ((S - D) /[(S+D)flhj x 100 number S________ C__oUbl (mOtL)

(alctptable range -

10%)

- IE4\\

t o

<C

.S0 I

S*J O

______J__Duplicate i

Dko.

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number

._(mL)

Blank (shouldbe-<0.l0mglL) x

-l.-;iOi.t C

o<. oq mCs',Gc.42 -lC Cto "Lek C0.021 cS.

OA-

.S b.

101 c

Ot A

'( O 6

< O*

'73 o~b.7-I

'I' Ii i*1 CnfiLot eAtr1C.L5 cJA( *c o.ocoZOS OS0608. a& _

i A5twl (ol

?~eA -- -PAPc.r I eS

c. U 5S llsam wereanal yi s

IEP com en gi utsuness Orwise nootdzS N~ote: All samples were analyzed in excess of EPA recommended holding timne (15 mrinutes) unless otherwise noted.

I Reviewed by Date reviewed I r-4k I

0Z.09-S 1

-4qtJ,.

Page 5 1 Page I

of 3 Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst l bth,_

Date analyzed lDtbo-II.cS Iodide reagent: I IJ Rl 6 q Acid reagent: I Fipii5b Calibration:

-I 0.10 mglL I

1.O0 mglL -

Reference standard number I rI'S I

I

&0 SSR)

I Note: For samples with a residual chlorine of > 1.0 mngfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Duplicate sampte precision:

t Sample Sample TD Sample characteristics Residual chlorine

%RPD - {(S; D) /I(S+D)1211 x 100 number fdd1 (mg/l)

(acceptable range - : 10%)

P $bPnci 1IvIllV.s S o.0o03

-Duplicate

0.

. D 4-L)e

-e Sam ple measurements:

Sample Sample [D Sample characteristics Residual chlorine number (mgiL)

..Blank (shouldbe=<0.10Lm)

Q

2

.*.*?...

___l___

C'.ao S- 0ii d

Ie.

L.

(0 sfOt bS411. 19 wlff ge C41 Y-.R io eto I.

05D 1II. Ib zV, tui wi-P

,-V.0 C2w n

Lh

(

At P

-O0 A1 N5 Dllale were aesLadc nded holin tm(

e n otherwise no t.Z tsoW IJ. ObL

Mr6it, W WVi h eonx 81 00A 0.000) 06 Ntote. All samples were analyzed in excess of EPA rccormcendcd holding time (15 hiinutes) unless otherwiscenoted.

Reviewed by IKqA2 Date reviewed I,t

.4 05

A1 Environmental Testing Solutions, Inc.

Page 52__

Page S of 3 Total Residual Chlorine

(EPA Method 330.5)

Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst IV_

I Iodide reagent: I N-.

I l

f.

l Datt analyzed I Dto-lt105-I Acid reagent: I N

I Calibration:

I 0.10 mg/L I

1.00 mg/L Reference standard number

I Note: For samples with a residual chlorine of > 1.0 mglL. the calibration range must 6e adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV) hMeasured value (NMV) oRS -MIV/TV X IO0 number (mglL)

(mg/I)

(acceptable range - 90 to I 10%)

WsS xi 0.50 o.o qi4 qZ.el q

Duplicate sample precision:

Sample Sa~mple ID Sample characteristics Residual chlorine

%RPD - ((S - D) /I(S+D)Y211 x 100 number (acceptable range -*10%)

>,.,Del;fe, MPr notoiL, sU",4-ss

S.

.0.- ;0;; 5"

-i Duplicate

.r:

D 4o.cOo26 4 _

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number (meJL)

Bnk (should be-< 0.10 mg/L)

Iso4'.

b7 oQ

^t~-I fi U (ic) a

, C10

.C 93 bSole'. JS IIICoiML f

e Z o.OI

'05soi)J.I ltn -tg(4L, W)l T YP lt

__,0 (oq toL 5 L.

O l. 1 6 '

_ __L_ _ _

bl5bb lo.o, lCr

' Il f-0 6SoAIl.lO wend in exesolP re ng tme min s o.eooiso Note: All samples were analyzed in excess at EPA recommended holding timc (15 minutes) unless othenwise noted.

Reviewed by l K

Date reviewed r 1

Y-l.OS

Environmental Testing Solutions, Inc.

Page 53 Page 3 o f Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.10 mglL Meter Accumet Model AR25 pH/Ion Meter Analyst I V

Date analyzed [

0 -11 K I

Iodide reagent: lI.

i Acid reagent: I Calibration:

~- _

0.10DmeI L

I 1.00 mgL Reference standard number Lo Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range niust be adjusted to bracket the chlorine levels of the samiples.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MV)

% RS = MV / TV x 100 number (rng1L)

(mg1L)

(acceptable nange - 90 to 110%)

. IWSS q-ID 0.50 aT h4 q

t79.

Du licatesample precision:

Sample SampIleD Sample characteristics Residual chlorine

%RPD = ((S - D) /[(S+D)12J1 x 100 number

__1_

(mgfL)

(acceptable ranee -*10%)

.j;ll.

21 hS

-Duplicate D

qq-Sam ple measurements:

Sample Sample ID Sample characteristics Residual chlorine number (me/L)

. K Blank (shouldbe =< 0.IOmgL) 6!Z~tOI0 VA 64 0 10%

40 1iB>

Z-00 2 q bZto1o.O3 INT n

1 tC e n-4..or t.Xo

. smolso P

_Ft6Ti 00i C.,-

.oCooqoI7 Note: All samples were analyzed in excess or EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratory control standard:

Reference standard True value (TV)

Measured value (MlV)

% RS bMV I TV s 100 number (mg/L)

(mg/L)

(acceptable rang - 90 to 110%)

lSsut 0.50 bq6O Reviewed by ktt Date reviewed r F 1.II -.05 i I

Environmental Testing Soluftions. Inc.

Page 10 PagejI of I Alkalinity (EPA Myethod 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst IlI77777 Date analyzed Titrate samples to pH = 4.50 S.U.

Titrant normality and multiplier determination:

pH of Normality Normality (N) of HSOl pH Factor or Multiplier Deionized Titrant check Begin End Total

- (5 ml Na1COj x O.05)/E

= (V 50000)/ 100 ml sample water reference standard ml ml ml

= 0.25/E

-Nx 500

- 4.5 S.U.

number

. number (E)

(acceptable range - 0.0I8 -0.0'2)

4. 1 L INR l Jg1 C3 11.9 915 (C

21 0 0J5l Laboratory control standard:

Reference True value Sample Alkalinity(MV)

% RSMI'lV/TVx1OD standard number (TV) volume Begin End Total Multiplier (mg CaCOIL)

(acceptable range (Mg CaCO3IL)

(ml) ml ml ml

-90 to 110%)

Ns5ze o100 0o0 23.3 32iq-D.q ca 9w 1 Duplicate sample precision:

Sample Alkalinity

%RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL)

J(S - D) I((S+DY211 x 100 number (ml) ml ml ml (acceptable range -

10%)

?aO51 fXBdr.4--6 s

3 10.6 s,-

F.e'D Duplicate 10o 0

.}2 1; 5 D

Afatrix spike recove Reference Spike value Sample Spike alkalinity (A) lstandard number (SIV) volume Begin End lTotal M iultiplier l(mg CaCOVL1) ll(mg Ca OV/L) l(ml) lml lml lmll WiS96 6l CO 0.0 ZOZ lzo.2 1.5 l 21Q Sample alkalinity (By l

Nleasured spike value (MV)

% R M

MV / SV x 100 (mg CaCO..)

NIV - A - B (acceptable ranZe (mg CaCOViL) 75 to 125%)

il*(-*IS.....

00 ED Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (m? CaCO/L)

W oCqL-r -

SSLO A cO JQOL. 2b.T 30

_ Z-Vxstj4-5A m

V6 oo I l

6 Lo l

ii i

b 41 6

~

3

_________{° Reviewed by:

Il Dtre Date reviewed: l.

°

Page.- V)

Page I_.oftC I Environmental Testin2 Solitions. Inc.

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed O Titrate samples to pH = 4.50 S.U.

Titrant normality and multiplier determination:

pH or Normaliq y

Normality (A) of HtSO4&

pH Factor or h ultiplier Deionized Titrant check Begin End Total

- (5 ml INa:COa x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml

- 0.2'IE Nx 500

=4.5 S.U.

number number (E)

(acceptable range - 0.018 - 0.022)

___q IiJ IZ ioofjg4 oo 1.o 17o O. 020(

io4 611L0.O- 0.0 =.0.ornI Laboratory control standard:

Reference True value Sample Alkalinity (MV)

% RS - MV / TV x lO0 standard number (TV) volume Begin End Total Multiplier (mg CaCOj/L)

(acceptable range (mgC3COL (l)(m) lml ml

-90to 110%)

1AS95 10 1oo0I 12.U Z2.7 9.4 Io&.q

/01

/00 28 Duplicate sample precision:

Sample Alkalinity

%RPD D Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S -D) I((S+D)ry1l s 100 number (ml) ml ml ml (acceptable range -

I 0%)

.o;-o5-6E.o tsjp) 100 ZI.' 2q2

.5.S 31 Duplicatc j

atn-31 D

£ 3-Z%

Aratr& spike recovery:

Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume I Begin End Total Multiplier (mg CaCO/L)

(me CaCOL (ml) ml 4 ml ml IN Gsse

61) j00 2q.9-S32.4j eb.O lo. q 03 Sample alkalinity (B)

Measured spike value (VIM

% R - hlV / SV x 100.

l (mg CaCOj/L) hV - A -B (acccptable range I

(mg CaCOIL) 75 to 125%)

Z3 6 61 4

90 Samolemeasurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO;L oioBeS A rni HLl 10 5~g 3

W5 O.k a

3 35 '.3 (1p43 C.o

&Z.

to I

I T_

0.0 6-.l 65.T, T_

I hH 1 j 6

16.?

1 1

I I S ISSW 14.0 1I I3.l I

___OS I

~ I

.5 1

3.1 -

1 33 Oto.I-CO A rna RS 14zo

-I I0 5

a 1 23.5 iq.s &

loI C.

'3o.V 3G.5ST

.91 _

(if Reviewed by:

I d1 I

Date reviewed: I O.20-0o5 o

l I

Page 20 Page 2_ of f i Environmental Testine Solutions. Inc.

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst Ifo l

Date analyzed Lii2Zi /q Titrate samples to pH = 4.50 S.U.

-Thxantxprmaliy and multip her determination:

pH Of NrmRNormality (N) of H1 SO 4 pH Factor or Multiplier Delonized Titrant check Begin End Total

(

E (IVx 50000)1 100 nil sample water reference standard ml ml ml 0.2S/E

- 45 S.U.

number number (E)

(acceptable range - 0.018- 0.023)

Labratrycontrol standard:

Reference True value Sample Alkalinity (MV)

% RS = NIV / TV x 100 standard number (TV) volume Begin End Total IMultiplier (mg CaCOJIL)

(acceptable range (mg CaCOJL)

(ml) ml ml ml

90 to 110%)

Ct-j6 2550 100 100 q

q4..7 lo-

/OI 16189 Duplicate sample precision: '

Sample Alkalinity

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L)

((S - D) nl(S+D)y211 x 100 number (ml) ml ml ml (acceptable rane -e *10%)

____bt0o4 Alc)a oa<

100 0o0 9.0 q0

.q s

Duplicate q0

/Oy io D2

.I'q.

Afatrix spike recoverv:

Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin

'End Total Multiplier (mgCaCOj/L) l (mg CaCOV/L)

(ml) ml ml ml aIkali9 ni nitl qn ityl 14°l 1I lr Sample alkaliity(B)

Measured spike value (MV)

% R NIV /SV x100 l

(mg C:COA/L)

NIV - A - B (acceptable range I

(mg CaCO,/L)

-75 to 125%)

q Jo 1 Sample measurements:

Sample volume Begin End Total Alkalinity.

Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOL)

O5cis:.Io l

I ol - 0i3 100 l 3.o 0 3Oq ?A l In.

790 I

l 0

_oK.

OR OS 0 to I8.

ll 31-S l

-l 0

5.

I34A l42.

2-8 OSo t.° 4

Z7-.

454 I _I___PPac_

I__,

1J.?

16./B 6. L I

- 1 oq A1..8 V

ii 2tO Reviewed by:

t re v Date reviewved: I of, -tOOS1

Page 2 1 Page _g of II Environmental Testing Solitions. Inc.

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst l77G C]

Date analyzed l 7> -q 5§ l Titrate samples to pH = 4.50 S.U.

Tr..~ormality and multiplier determination:

p iH of v

Normality (N) of HISO pH Factor or Multiplier Deionized Titrant check egil otal (5 ml Na COj x 0.05)/E (Nx 50000)1 100 ml sample water reference standard ml ml ml

-rN 500 4.5 S.U.

number number (E)

(acceptable range - 0.018 - 0.02 Laboratory control standard-Reference True value Sample Alkalinity (NIV)

% RS - INlV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOj1L)

(acceptable range (mg CaCOjlL)

(ml) ml ml ml

-90 to I 10%)

if s 2 5 100 100 0.3 q 2 1(.

/

If Duplicate sample precision:

Sample Alkalinity

%RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S - D) /R(S+Dy21 x 100 number (ml) ml ml ml (accepuble range -*h 10%)

3 b

336.S 1

-2) 10 S

/ 110 Duplicate

&)

I'i.

06u

.Z1 D

l_

Mlatrit spike recovery:

Reference Spike value Sample Spike alkalinity (A) standard number l (SV) lvolume Begin End Total Multiplier (mCaCOvL (mg CaCOL)

(ml) ml ml mll

/A0 77'iq6

/10 5Z) vO bh Z

i 210 Sample alkalinity (B)

Measured spike value (MV) l R

i=V

/ SV x 1 00 S(meg CaCOj/L)

MIV - A - B l

(acceptable range I

I (mg CaCOV1L) 75 to 125%)

lobD 110l o9?

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOIL) oSot0.o7.0 l)XY

/ 1(x) 19.0 lZo

,0 q

94 31 OSO Ot.o l

2

/

I 22,1 25;1 j

)l 31 105GO 1I.-

3 T,

I0 TZ54_12_2_

T 2

I cso'a0o7.otlI-< 6I IjiJ Q

12 8 O L

____M 1

9 ososotl T

U.o2.

1U..

1 --W. 1 Th

-(

1os1o3.

Ll A, F1

- 1 9

lOstcN i.O I

I0 I2.51 31 I 36 L 1-Iav-7 1I4.1 1f I 151 CYS0o,01.o 01 3 1.

14 172ZiD -we19 6

II/

Reviewed by:

_ _ _ _ Date reviewed: lot Uo I

Environmental Testing Solutions, Inc.

Page ZZt.

Page 4 orfz_

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L Date analyzed l Ots 14N55 Titrate samples to pH = 4.50 S.U.

Titrant normality and multipler determination:

PIQ _Normality Normality (N) of lIaSQ, pH Factor or Mtultiplier Deionized 7r-nt

-- cck_

Begin End Total

= aml iaJs00)E

= (N x 50000 t00 ml smple watcr reference shnodaird imt-ml 0.2SIE v Nx 500

=4.5 S.U.

number number (E) e.0OIS

- 0.0212)

Laboratory control standard:

Reference True value Sample Alkalinity (MV)

% RS -

N1V/TV I OO standard number (TV) volume Begin End Total Multiplier (mg CaCO9JL)

(2cceptable range (mg CaCOj/L)

(ml) ml ml ml

_ 90 to 110%)

I s p sp 100

'oo.2,0 3,LS J

16 Y

/b0 16 ?,

Duplicate sam le precision:

Sample Alkalinity

%RPD -

Sample

, ample ID volume Begin End Total MIultiplier (ng CaCO,/L) l(S-D)/I(S+lD)P7Ij s 100 number (ml) ml ml ml (acceptable range -

10%)

O~

0 Co c'

(j 0

i Lq2,3

/(j 10'{

S II:

Duplicate J

0° IV.

1Q.L D

-/I AMatriv spike recovery:

Reference Spike value mple l Spike alkalinity (A) standard number (SV) volume l Begin End Total Multiplier (mg CaCO,/L)

(mg CaCOIL) I(ml) ml ml ml IASS SR2) 6D 0

JQO 6-1 5°I.3 15. 3

)JOq 1,

I

/410 Sample alkalinity (B)

Measured spikevalue (t V)

% R -

V I/SV x 100 l

(mg CaCO,/L)

MNIV

- A - B (acceptable ran.e I

(mg CaCO3/L)

-75 to 125%)

I

.7)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample nutmber Sample ID (ml) ml Multiplier (mv CaCOVL)

I ossb.cORz&

1 l

6.i 5

2)

-rvh 1/1P 6

OSot0 tO.O'-q I_

I 41.

-YSC_

0 ty 5"'

31.0 5d,14~2.

Osct. Io -O I

3S.

10 1a6lS.1 41 "S-qO 05o.10.05 3Ox _

q rA 6Qtf1&.O

.1-14 10-i qo g/bo c.QlXlCA ;2 1

1 oo 0°V T4L lQu I

_1q

1.

Osopbo.07-TA 6~)

l

~T ht1b0

1 (O.

It's 0(, rj to.

-rV60A W16) I 0Id.1 1qS.91 7 1

1 ItI Reviewed by:

Dteiw:::-

Date reviewed: IO mr2o-Q 1

Page 2 3 Page __

f f

Environmental Testine Solutions. Inc.

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst M )%,

Date analyzed l Titrate samples to pH = 4.50 S.U.

Titrant norality and multiplier dt ination:

pHr NOMM

~

Normality (N) orH2SO, pH Factor or Multiplier Deionized Titrant check Begin nr-S_ C0 X 0.0;)/E

= (NI 50000)/100 ml sample water reference standard ml ml ml

=0.25

-45 S.U.

number number

(_E)

(acceptable ranage - 0.018 - 0.022)

Laboratory control standard:

Reference True value Sample Alkalinity (NMV)

% RS =MV I TV x 100 standard number (TV) volume Begin End Total iMultiplier (mg CaCO3/L)

(acceptable range (Mg C2CO3L)

(mI) ml ml ml

-90 to I110%)

I AcSPS 100 100 7-q 37.fo II 0.C) 1/ 1

/

Duplicate sam le precision:__

Sample Alkalinity

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL)

((S - D) Ii(S)Dy12il s 100 number (ml) ml ml ml (acceptable range -

10%)

__3___

ASON 12 j /O.') s37.L 40 Q.L D

4O-oo -4 z Duplicate 3

3 D

i,_37, Mfatri& spike recovery:

Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CsCO3IL)

(mg CaCO3L} )

(ml) ml ml ml ti 25

-496 ER) 100 1..

1o l2.i 0-0 1 O.'J 2.I Sample alkalinity (B)

Measured spike value (NIV)

% R - MV I SV x 100 I

(mg CaCOVlL) l MV - A - B I

(acceptable range I

F (mg CaCOVL) 75 to 125%)

4q Sample Jneasurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (mI) ml ml ml Multiplier (me CaCO3IL)

OlSOblO.a 0

I A Ql/

(o 12.0 l/.0l lo. C

_______I 5? ___

IOA0

? O l/

C I

JCS,20 I

2.OSc 8.7S I

Sk 1A I

q.

30.3 656 Il

o O9o +/-2JiL.

CAM zct.I

'2.0 b.q IC?3 120 l

l l

C Ae l

Reviewed by:

Dlte re e

I Date reviewed: l OL 2,1 t5

Pago 1:.

Page L of I Environmental Testing Solutions, Inc.

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL-1.0 mg CaCO3/L Analyst l

l lII]

Date analyzed Titrant normality and mutplier determination:

Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2E

= (V 50000)/ 50 ml sample number number

_E)

(acceptable range -0.0 IS -0.022)

-Ns 1000 WI£RZ1

_9ijZjs_

D O

'q ?

0.b

-2_.

Laboratory control standard:

Reference True value Sample Hardness (MV)

% RS = MV I TV x 100 standard number (TV) volume Begin End Total Mlultiplier (mg CaCOAIL)

(acceptable range I 52' mgCsCOt)

J lJ ml ml ml

_ 90 to ItO%)

ip -A 40-50 Ct-4 Ilu~o J.9

-2 0('O 39 CP 9

50)

Duplicate sam ple precision Sample Hardness

%RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L) f(S - D) II(S+DY21) s 100 number (ml) ml ml ml (acceptable range - a 10%)

LO

.t SSLO -t 5U j1.@ i.'4 jo s_

j Duplicate D

S4$

1 Zo D

L 3 Matrix spike recovery:

Reference lSpike value lSample l Spike hardness (A) standard number (SV)p volume Begin End j Total M1ultiplier S (mg CaCd A)

I(mg OLCOV)

I(ml) Iml Iml ImlI I

s a 4

I' a

1 55 LR 1na i.0 II.

Sample hardness (B)

Measured spike value (NIV)

% R -,MIV I SV x 100 (mg CaCOqjL)

NMV - A - B (acceptable range ll (mg CaCOj,/L)

_75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg Caco()

Blank l -

l l (shouldbe 0 mgCaCO,/L) l Sb 0.l 0.0 0.0 ZO.( T _

l 02 jC75 A fn 5

V12_

G.-+

R

{Z -9 1 A~

.l DocWT 6 h

~

URo Vs6 4___0 fl.Q 3JiLJ L *°

____4._

Note: If >I5ml of titrmnt is used, sample must be diluted.

Reviewed [

Date r O

Date reviewed I o' W.6 t5 I

Page lt Pagel of 5

Environmental Testing Solutions. Inc.

Total Hardness 4

t p

t e

Z_

(EPA Method 130.2)

CA) l O Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst l,@- I

nt'?/

Date analyzed I D, 14 _UJ5 Titrant normality and multiple dtriation:

p deermi Titrant Normnality cbeck Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml

- 0.21E (IVx 50000)1 50 ml sample number number (E)

(acceptable range - O.01s - 0.022)

=Nx 1000 11 J Rtc, sR 3

O.O l O0 1.

.0tO t

Laboratory control standard:_

Reference True value Sample Hardness (MV)

% RS - NIV / TVx 100 standard number (TV) volume Begin End Total Mfultiplier (mg C2COj/L)

(acceptable range (mg CaCOO/L)

(ml) ml I ml ml

- 90 to 110%)

155 AA7 40 50 ID0

. O 12 Z

-27.Z I*ZO 44 u10o-o7.

I 4.*.

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml lMultiplier (mg CaCO/L)

I /; t) 1 (sbould be - 0 mg CaCOIJL)

D) 0 -f O-

2) 0 ND OS06ta.c17z SOO la__

U.?.

'qt I

4.4

-S so(Z 0,.QZ.

Sa m (o )

Z._.

3q.-2 4j.o 8

GO(OC 0(.. 02.

Sa ba Is c~ 0, 80__

a,z.. _l q t VA1 ea_

6o t o b lO. G; 3 S@

Q>

4 z.4

=

O5WSZ3'o

,G4F,6e 3.A OS 0 b 10. O3 $

esmX£.(h r W 38 ts 42AL s.9 it bes t0.

0 2A 5

.fiO'

1.

t 6

OSOC.Ot.2&

PAIP CooQ A5..

34-1Z. ()

45Z=

Os0oLOL.

O. 4 C

PA tr 00%

_IO 0

1o 42 15 520 05<t66L04P (3p itm 0.0 3-

)-15a ISO5 Note' If > I SMl of titnrnt is used, sample must be diluted.

Reviewed l]

b y :

I L ( Y.J-Date reviewed E 3 1 O S

Environmental Testing Solutions, Inc.

Page Iq Page 7-of 5-Total Hardness (EPA Method 130.2)

Matrix: Water, IDL = 1.0 mg CaCO3/L Analyst Date analyzed [I i I5 II]

Titrant normality and multiplier determination:

Titrant Normality check Begin End Total Normality (N1 of EDTA pH Factor or Multiplier reference standard ml ml ml

- 0.21E (Na 50000)/50 ml sample number number (E)

(acceptable range 0.018-0.022) iNX 1000 Laboratory control standard:

Reference True value Sample Hardness (MV)

% RS - MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOAL)

(acceptable range (mg CaCO/L)

(ml) ml ml-ml

_ 90 tol 10%)

IV=S ZZ' 40 50 vt.

23i 2.o 200 IC)

I h7Ol Duplicate sam Ic precision:

Sample Hardness

%RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL)

((S - D) /[(S+DY2[) x 100 number (ml) ml ml ml (acceptable range-i 10/)

cfso - Z9 t rNr o

a, 3

l.S n n S tSL -Z sd

-i*1 2

f Duplicate D4

l. S

.1 D

Matrix spike recovery:

Reference Spike value Sample Spike bardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO/L)

_(mg CaCOj/L)

(ml) ml ml ml I

oIS& 'ZZI

40.

50

-,"2l.Z_~

Zo 1,1 A t ll.

=7 14.

Sample hardness (B)

Measured spike value (NM)

% R -MV / SV x100 l

(mg CaCOIL)

MV V=A-B (acceptable range (mg CaCO,/L)

-75 to 125%)

_ample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml nml ml Multiplier (mgCCOC3L)

(should be - 0 mg CaCO,/L) l Po(4.OS t

X Ca)

So 23.2. 3t.0

1.

0 1ICC.

l1cIO olot.0t. 2.4 fl&,p

(

3180 sem -Il 1l160 1

os.tolo tMsw()

llI

.'S 1\\

.0 1(*

l l

l dSX azo(od.jO l CzT9 l

4.2.

zo.zT z.o 1

1 I

l`50b64.l 7-9s

_L

I

-IS VS

-. I l-u l

0sco(o1.

7 0

t4w T __2_._2_

1.1 I

4 cosol.o e 0 I btoe1m1w 2

Z Zo.3

?_

7.0

&JVV" QAN 7Z35L Note: i lml oftitrant is used, sample must be diluted.

Reviewed l

l by:

lI Date resiewed I OC-?200S I

Page ZO Page 3

o Environnmental Testin2 Solutions. Inc.

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed li c4IIJ Titrant normality and multiplier determination:

Titrant Normalify check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.21E

(,Vx 50000)150 ml sample number number (E)

(acceptable raoge-0.018-0.022)

-Nx 1000 Laboratory control standard:.

Reference True value Sample Hardness (MV)

% RS -

v1V / TV x 100 standard number (T) volume Begin End Total Multiplier (mg CaCOj/L)

(acceptable range (mgCaCX)

(ml) ml ml nl

-90 to 110%)

1M& 2Z1 40 50

?4 3

t.

Ito Duplicate sam ple precision:

Sample Hardness

%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL)

I(S -D) 1[(S+DY2lj x 100 number (ml) ml ml ml (acceptable range -fi 10%)

_C____C_.OL

_ -__6_

sc5 'v.3 ao 2.t zO S;_

Duplicate 24 Z

5A 31,C4 Z

4 e

D CaC3.e70 Afatrix spike recovery:

Reference Spike value Sample.

Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mgC&COi/L) l(mg C&CO31L)

(ml) ml ml lmll

\\IiSs 12N-72c M-S SO Z--3 41

20) I8 Sample hardness (B)

Measured spike value (MV)

% R - MV ISV x 100 (mg CaCO/L)

MV A - B l

(acceptable range (mg CaCO/L)

-75 to 125%)

5;

'qS.O7o Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) nml lml ml Multiplier (mg CaCO3L)

Blanka

)-

ICvStb@1,iL lE;J; eiwbnb 0

50 35-3 lts* i l

.A ZOX lOBc at I C D) 50 1

38.1 3.o l

G

°S~t-16°alt 5ox b

ZU

.0 38.1 40.-7 A.O 40 bSttwo I ALs aa I 5° qo 01 43.B NO G

bI 50btoISI-AlAA 002.

l 50 0

3-1 3.1 l 0-xpI-.

0 AU-O 01?,

rt is.

A0

.e QD

.qI 1

q.j II ocoC1e-o2

ALCOA, j

5 i° 170 j

Ita.) lb a

0! oso&S.O'I ALtA 01 O

(3_

5_

l 17.0 IMl l

.l l Note: If >1 SmI of titrant is used, sample must be diluted.

Reviewed by:

14 Date reviewed I 20- OS I

Page P _ _

Page Lt 0f 15 Environmental Testing Solutions, Inc.

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst FIM II Date analyzed [

4-0S l

itrant normality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Mlultiplier reference standard ml ml ml

- 0.21E (N x 50000)/ 50 ml sample number number (E)

(acceptable range-0.018 0.022)

-Nx 1000 Laboratorv control standard:

Reference True value Sample Hardness (N IV) % RS - NIV I Tv s 100 standard number (TV) volume Begin End Total NiMultiplier (mg CaCOjIL)

(acceptable range (mg CiCOjIL)

(ml) ml ml ml

- 90 to 110%)

XWaSS aX7 40 50 X5t;.; 2.l R2 4-10°5 Duplicate sam le precision:

Sample Hardness

%RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)

((S - D) I(S+DY21) x 100 number (ml) ml ml ml (acceptable range 10%)

50W10 Q

)

O Jc2/,

d?3.5 2.3

0 s 46 g

~

Y e

Duplicate SO

.35;.

l ao D f Matrix spike recovey:

Reference Spike value

-Sample Spike hardness (A) standard number l (SV) lvolume lBegin lEnd lTotal l Nultiplier l(mg CaCOs1L)I I (mg CaCOiIL) l(m ml ml ml IfISS ;t'7 q o 50 l2 3.l7C l

a0 l

t(

qO Sample hardness (B)

Measured spike value (MV)

% R - MV / SV x 100 l

(mg CaCOIL)

MV - A -B (acceptable range I

I (mg CeCOVL)

-75 to 125%)

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg CaCO/L)

Blank

- m C g

l ~~(should be - 0mgCaCOL) lI l

l I-lA Al._-_______

50

17. to 31.l 2

- 0 icAQ-bXtik 60 ZI.s S 35.9 LYO llI 8

PPAk -i.Q A, 50 35.B q0.j 1 l___

6S p.oe-o OS 1

4.s 4

_MS-A ov~oe-s I

O 140-X.1 4

13 4

K__

tMi-GnooS l

666-

-o50 4.0

/.0 80 lA___

o_

l4 0lo l

50 4.0 8

4.',

l_

l 9_

Mis-6 00,'i 50 G.4 1l -7 1

I dlsq tiN ao.f6X1 o

Ia.7

15. v.1 ?

4, i11L I_______

I.

I I.

. I I

v A

Note:If >lSmloftitrantisusedsamplemustbediluted. Reviewed by:

j

-]l Date revee l a-1&0

Page 22 Page 5

of S rnvirnnmPntal Testing Solutions, Inc.

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL 1.0 mg CaCO3IL Analyst Date analyzed 06-lq14 5

Titrant normality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E (V x 50000)1 50 ml sample number number

_ IE)

(acceptable range - 0.018 - 0.022)

= X x 1000 Laboratory control standard:

Reference True value Sample Hardness (NiMV)

% RS - A=V / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJL)

(acceptable range (mg CaCO/L)

(ml) ml ml ml

-90 to 110%)

xZvss dA 7 40 50 a1. 7 2q 2 -3 0

46./

50 go4.O 411 0

O o

aCO Duplicate sam ple precision:

Sample Hardness

%RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L)

((S - D) 1I(S+DY)21J 100 number (ml) ml ml ml (acceptable range 10%)

ii:c- *1b-Q~

-O 150.

e 17.3 d.S 3 R 0 s

Duplicate 50

17. 3 5

D

L el Mdtrix spike recovery

Reference Spike value Sample l Spike hardness (A) lstandard number l (SV) lvolume lBegin l,;d lTotal l Aultiplier l(mg CaCO31L)l (mg CCOAc)tL (ml) t5)ml3m*lm IJ &Tmi

.:1S -;855 7

4 0 50 c1.:d 41 dD l

Sample hardness (B)

Measured spike value (MV) lVISVx 100H n

(mg C&COjL)

I hlV-A - BI (aceeptable range (mg CaCO31L)

/@

l

-75 to 125%)

l Sample measurements:

ISample volume lBegin lEnd lTotal l

Hardness Sample number Sample ID l

(m) ml ml mll Multiplier (mg CaCOIL)

I

_I ~(should be 0 mgCaCOVL)l I.

I l

li1;-A oto-i6-CS 50 a.o (P.O. 30 1 4 l

O l

l w-EW s M-11. O 50 1 0.1 134-.1 4. I AO OafS t1% &C.

OQY1-

_S _60

_34.a U.4 A

_a__

_ao 1I Lek IC I 1 2-1b 1-.

1501 15.

1 QZ I-I_

I Note: If >1 5ml oftitrmnt is used, sample must be diluted.

Reviewed l1

' Dat reviewed [ (j 6-20-S I

Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water U

c9 1.0 0.9 0.8 0.7 0.6 0.5

0.4 1.0 0.9 0.8 I

I I

I r I I

I I

i I

I I

I State and USEPA Mandated Control Limits Limits Set According to +/- 2 Standard Deviations I

.I I

I I

I 0.7 0.6 0.5 0.4 l

I I

I l

I I

1 1I I

I I

I a

I USEPA Warning and Control Limits Limits Set According to 10h and 25h Percentile CVs

~~~I I

I I

I jr4T dat et%

SV

¶I.

'5 Test dateI 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC25)

Warning Limits (mean IC25 SA1)

. Control Limits (mean IC25 4 SA25 or 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water Telt sumber Test daft 7-day ICS, CT (dLKCI)

WLCKCD State and USEPA Laberataty.

Lb.oratery S

Control Lmits S.lo Warning Units SA.S Coetro! Limits Skis CT.2S CT+2S CT-SA.

CT+Sr+f Cr.S,.

Cr+S,,,

USEPA USEPA Wandtng Umts S.

central C

Lnits CT-SkSi CT+SA.19 CT-SA.

CT+SA."

Cv 1

10-19-04 2

11-02-04 3

11-09-04 4

11-30-04 5

12-07-04 6

01-11-05 7

02-084-5 S

02-12-O5 9

03-01.05 t0 03-01-05 11 03-08-05 12 03-15-05 13 03.29-05 14 04-12-05 15 04-26-05 16 04-26-05 17 0543-05 18 05-10-05 19 05-17-05 20 06-070-5 0.52 0.68 0.54 0.54 0.64 0.63 0.62 0.62 0.61 0.61 0.69 0.62 0.67 0.61 0352 0.58 0.61 058 0Q50 0.62 0.60 0.58 0.37 0.58 0.59 0.59 0.60 0.60 0.60 0.61 0.61 0.61 0.61 0.61 0.61 0.61 0.60 0.60 0.60 0.12 036 0.84 0.07 0.53 0.09 0.40 0.76 0.07 0.51 0.08

0.42

0.72 0.07 0.50 0.07 0.44 0.73 0.07 0.51 0.07 0.45 0.73 0.07 052 0.06 0.47 072 0.07 0.52 0.06 0.48 0.72 0.07 053 0.06 0.49 0.71 0.07 0.53 0.05 0.50 0.70 0.07 0.53 0.06 0.50 0.72 0.07 0.54 0.05 0.50 0.72 0.07 0.54 0.05 0.51 0.72 0.07 0.54 0.05 051 072 0.07 0.54 0.06 0.50 0.72 0.07 0.53 0.05 030 0.71 0.07 0.53 0.05 0.50 0.71 0.07 053 0.05 0.50 0.71 0.07 053 0.05 0.49 0.71 0.07 053 0.05 0.49 0.71 0.07 053 0.67 0.13 0.65 0.12 0.64 0.12 0.65 0.12 0.66 0.12 0.67 0.12 0.67 0.13 0.67 0.13 0.67 0.13 0.68 0.13 0.68 0.13

0.69 0.13 0.69 0.13 0.68 0.13 0.68 0.13 0.68 0.13 0.68 0.13 0.67 0.13 0.67 0.13 0.47 0.46 0.45 0.46 0.47 0.47 0.47 0.47 0.47 0.48 0.48 0.48 0.4S 0.48 0.48 0.48 0.48 0.47 0.47 0.73 0.70 0.69 0.71 0.71 0.72 0.72 0.73 0.73 0.74 0.74 0.74 0.74 0.74 0.73 0.73 0.73 0.73 0.73 0.23 0.22 0.22 0.22 0.22 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.23' 0.23 0.23 0.23 0.23 0.23 0.23 0.37 0.83 0.27 0.36 0.80 0.26 0.35-0.79 0.26 0.36 0.Q1 0.26 0.37 0.82 0.27 0.37 0.82 0.27 0.37 0.82 0.27 0.37 0.83 0.27 0.37 0.Q3 0.27 0.38 0.84 0.27 0.38 0.84 0.27 0.38 0.85 0.28 0.38 0.85 0.28 0.38 0.84 0.27 0.38 0.84 0.27 0.38 0.84 0.27 0.37 0.83 0.27 0.37 0.83 0.27 0.37 0.83 0.27 0.33 0.32 0.31 0.32 0.32 0.33 0.33 0.33 0.33 0.33 0.34 0.34 0.34 0.33 0.33 0.33 0.33 0.33 0.33 0.87 0.20 0.84 0.16 0.83 0.13 0.85 0.13 0.86 0.11 0.86 0.11 0.87 0.10 0.87 0.09 0.87 0.09 0.88 0.09 0.88 0.09 0.89 0.09 0.89 0.08 0.88 0.09 0.88 0.09 0.88 0.09 0.88 0.08 0.87 0.09 0.87 0.09 Nble:

7-d IC, day25% hkins eoncentration. An estnoatbn orthe concentntin ofpotassim cheide that woud cause n25% reduct'in hi Pimephis owtb for the teat popution.

CT-Cratnal tendency (moam lC2,).

S - Standard devWtmn ofthe IC,, wa)es.

Laboeatory Centrol ant Wanrng Limits Labotorycontrol and w ig mb ere atblshed using the standard dction of the IC vaes cemspondig to the 10th and 25th prcent& CV.

These ranFes are more rtigent dhn the control and wan g lnk recommended by USEPA foe the teat method and cadpot. -

9AIj - Standard detion correaponding to the 10' perventil CV. (SAI9 - 0.12)

SA2 - Standard deiition corresponding to the 25' perctile CV. (SA2s - 0.21)

USEPA Centrol and Warnrin Lbibt SAki - Standard deviation coruponding to the 75' pucentile CV. ( Sp-" - 0.38)

SA9 - Standard devation eeresponding to the 90' percente CV. (SA6 - 0.45)

CV - Coefclment of isoation ofthe IC-, values.

USEPAn 2i0g toW-hg miE rMetd VurbftyktWhoL-Met ToxkkyAppkbti tWbdu the NtbarobantDkcrbe Emkatb& Fro.

IErA tAll-R-,US Snvt cbtaltot-tin AYey.. Chkrnttt OH.

O,

n...

IncIldj,_.

qurl S yf

. le0 064705

Environmental Testing Solutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pitnephales pronielas using Moderately Hard Synthetic Watcr 1.0 0.9 0.8 2

0.7 0.6 In q04 U

0.5 I

I I

0.4 F 0.3 -

0.2 1 1

1

. I I

I I

1-1PS" NNA

\\3N' O-."-

%-1 s

PJO~ e

%-5-0 %Tst Odtes

%N.

o-X

%0 op rj°5 Os" to Test date

7-day 1C25= 25% inhibition concentration. An estimation of the concentration of potassium chlorde that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC25)

Laboratory Warning Limits (mean IC25 + SA.10 iSA 10= 0.12)

.Laboratory Control Limits (mean ICs

+ S A25 A25= 0-21)

USEPA Warning Limits (mean IC25 + SA75, SA75= 0.38)

USEPA Control Limits (mean IC25 A

S90, SA90= 0.45)

Environmental.Testing Solutions, Inc.

Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Control Control M~ean Test number Test date Snrvl Growth Survival Growth CT CV CT hlSD PMSD CT (0/)

for Control Growth (mgtlasvae)

(mglarVi)

(%o) for Control N rowth CV (%)

(%)

for PNISD (%o) 1 10-19-04 2

11-02-04 3

11-09-04 4

11-30-04 5

12-07-04 6

01-11-05 7

02-08-05 8

02-12-05 9

03-01-05 10 03-01-05 11 03-08-05 12 03-15-05 13 03-29-05 14 04-12-05 15 04-26-05 16 04-26-05 17 05-03-05 18 05-10-05 19 05-17-05 20 06-07-05 Note:

CV-I 100 100 100 100 97.5 100 97.5 100 100 100 100 100 100 100 100 97.5 100 100 100 100 0.757 0.756 0.609 0.689 0.687 0.797 0.687 0.712 0.749 0.889 0.735 0.533 0.676 0.702 0.742 0.702 0.760 0.772 0.858 0.848 0.757 0.707 0.703 0.699 0.716 0.712 0.712 0.716 0.733 0.733 0.717 0.713 0.713 0.715 0.714 0.716 0.720 0.727 0.733 1.1 10.9 7.8 15.7 7.6 8.7 3.9 5.8 11.8 4.5 8.3 3.1 12.5 9.8 3.9 7.2 2.5 10.7 6.0 3.3 0.09 6.0 0.15 6.6 0.11 8.9 0.10 8.6 0.09 8.6 0.10 8.0 0.11 7.7 0.11 8.1 0.17 7.8 0.12 7.8 0.08 7.4 0.06 7.8 0.12 8.0 0.10 7.7 0.09 7.7 0.10 7.4 0.10 7.5 0.10 7.5 0.06 7.3 0.09 12.3 19.3 18.0 15.1 12.7 13.0 15.5 16.0 22.7 13.7 11.0 10.5 17.4 13.9 12.3 13.5 12.9 13.5 7.4 10.1 15.8 16.5 16.2 15.5 15.1 15.1 15.2 16.1 15.8 15.4 15.0 15.2 15.1 14.9 14.8 14.7 14.6 14.3 14.0 Coefficient of variation for control growth On aveage, the CV for control growth is 73% in Environmental Testing Solutions, Inc. Pimrephaler chronic toxicity tests.

Lower CV bound determined by USEPA (10I percentile) - 3.5%.

Upper CV bound determined by USEPA (90' percentile)

.20%Y MSD - Mirnmum Signifkcnt Difference PMSD - Percent Mininnu Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent diffemence between the control and treatnent that cinbe declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 14.0% from the control Lower PMSD bound determined by USEPA (10' percentile) - 9.4/.

Upper PMSD bound determined by USEPA (90' percentile) - 35%.

CT - Central Tendancy (mean Control Crowth, CV, or PMSD)

The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephalet growth in chronic reference toxicant tests USEPA 2000. UnderstaMding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.

EPA-S33-R-00-003. US Environmental Protection Agency, Cincirnnti OH.

Organ fsms obtained from Aquatic BloSyserns. Inc.

0)6407-05

Environmental Testing Solutions, Inc.

Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 1.25 1

-S C4 o

es 0

Ia a

.=

o.

.o

=:

U E

1.00 I 0.75 0.50 I

I I

I

~...................................................-..........

_.._~

~..._................_...._..

Minimum Acceptance Criteria (0.25 mg per surviving larvae)

I-I I

I I

1 I

I I

I 0.25 S =

o.4 I

u Is o

CJL) 0 U

40 30 20 10 0

North Carolina Acceptance Limit (< 40.0%)

Kentucky Acceptance Limit (< 30.0%)

~ *----;....----.'.----.......................................................................................................

E I

Test date Control Growth or Coefficient of Variation (CV)

Central Tendency (mean Control Growth or CV)

. Control Limits (mean Control Growth or CV + 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 40 30 20 10 0

I I

I IIIII Test date

- PIMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean PMSD)

.Lower and Upper PlNlSD Bounds Lower PMSD Bound ( 1 0h percentile) = 9.4%, Upper PMSD Bound (90tb percentile) = 35%

(Lower and upper PMSD bounds were determincd by USEPA for the method and endpoint.)

QA Reference Toxicant Summary Laboratory name:

Address:

Contact person:

Test species:

Reference toxicant:

Test type:

Dilution series:

Type of control water:

Reporting period:

Environmental Testing Solutions, Inc.

PO Box 7565 Asheville, NC 28802-7565 Jim Sumner Pimephales promelas Potassium Chloride (KCI)

Chronic 0, 300, 450, 600, 750,900 mg KCI/L Moderately hard synthetic water April - June, 2005 Test date Organism age IC2 5 (mg/L) 95% C.I.

(mgJL)

Average control growth (mg/imitial larvae) 10-19-04 11-02-04 11-09-04 11-30-04 12-07-04 01-11-05 02-08-05 02-12-05 03-01-05 03-01-05 03-08-05 03-15-05 03-29-05 04-12-05 04-26-05 04-26-05 05-03-05 05-10-05 05-17-05 06-07-05 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 24-hours old 517.0 683.9 537.6 540.8 638.8 627.2 616.4 619.7 612.2

'607.5 689.2 619.0 667.7 614.2 523.6 579.9 610.1 580.6 504.0 618.4 273.2 536.8 129.8 333.7 537.5 558.4 490.7 503.9

,448.4 499.8 606.8 520.6 311.7 453.8 406.7 99.4 563.3 513.3 418.4 529.7 690.2 692.3 674.1 639.6 718.8 663.4 667.8 687.9 682.4 651.9 807.1 686.7 796.5 683.3 610.8 655.4 637.9 658.0 615.9 651.4 0.757 0.756 0.609 0.689 0.687 0.797 0.687 0.712 0.749

0.889 0.735 0.533 0.676 0.702 0.742 0.702 0.760 0.772 0.858 0.848

Environmental Testint! Solutions. Inc.

Pagel1 of '

Environmental Testin2 Solutions. Inc.

Page 1 of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)

Species: Pinephalesjpromelas PpKCICR Test Number: -tJ6 Dilution preparation information:

Comments:

KCI CHM number:

LA" ft8 Stock preparation:

50 g KCVU Dissolve 50 S KCI in I L Deionized water Dilution prep (mgfL) 300 450 600 750 900 Stock volume (ml) 6 9

12 15 IS Diluent volume (tnL) 994 991 9SS 985 982 Totl volum (mL) 1000 100 1000

. 1000 1000 Test organism information:

Test information:

Organism age:

TZ.vS Por4 03.I_

Randomizing template:

fe4FP Date and times organisms atc-CAo-S VS ro tS~ c Incubator number and 3

were born between:.

._shelf location:_

Organism source:

A6%

B$c~rJ) csa S Artemia lot number:

6 to0l T Transfer bowl information:

pH =

SU Temperature =

C Total drying time:

1+

__S3__

Z4 1 C.

Date / Time in: C,.-i4.cJ 2

Average transfer volume:

t

.. Q Date / Time out:6 iS-m-L5 Oven temperature:

toOC Dailyfeeding and renewal information:

Day Date Morning Afternoon Test Initiation, MNHS Analyst feeding feeding time renewal, or batch used time termination time 0

o6z51oS I

X CoQSA 4

1 b

O I oo O3cdocMC e5A d

2

__-o__l 1555 1KS7:54 MO q-uSe.. A 3

0

-tS%

s.tob

%zsSk

&tCcA-CUSCL 4

4 cia-tb-ds Io'sS50 l3

,.- C-Os 1\\

j 5

____il2.-c5 soQZ 15 134AO N-C)c2*Os EK 6

oi 5-OS olso 8

l I

oL-Ot-'s jj 7 l l S.-:......;'...... 31 Control information:

. Acceptance eriteria S

Summary of testendpoints:

%Mortality:

O20h 77-dayLC 5

l Average weight per initial larvae:

O a. EIR

.<.- ;l NOEC l

5O Average weight per surviving larvae:

Z., 0.25 g/larvac LOEC to ChV I C 15

Environmental Testing Solutions. Inc.

Page 2 of 5 Species: Pimephalespromelas PpKCICR Test Number.

(ovo Survival and Growth Data Day Control 300 mg KCIIL 450 mg KCVL A rB C

D E

F G

H I

J K

L 01 10 10 10 /I0 /0/l 1/

/O/0

/0T0 M° l IC l

10) l I) CY I

It -O lIC/)l/

3 IC

/O1 t

O O

/0

/O

/6

/)

to)

-/0 4 ~

/0 I0

/ O

/O 1 0 10 16'

/0

{

/OlbI0 S

10 10 10 10 10 10 to 10-lo lO 10 to IC)O /)

l O /to lIt O I O '

l I0 1I b IG

/0 16

/06 j

/0 /b /

/_

0 A-Pan wightmg_

AnlytACSl4<o1il tlul 87 lq.52 NA.!5 tNA3 I's-8lo lqg4sqIt,3l B - Pan + Larvae weight Aals:

uj S.so 23,3Z 22.4t2 22.8i 23,1b 22.Sl 2276 7b % bA ZZA

.2.

91z.8ZZ13 22.34 Laaeweight(mg A-8.tvi g.tA V.,S1 8-o1 S8.Wq

-1.5a 1.83 t.-W 1.91 8.28 &.1o 7.S3 Weight per Initial number of larvae (mg)

- C I Initial number of larvae 9"

0cK I 0 I "@ 1 N%,1 j

O1 O r

O O

.O

0 DI Average Percent weight per reduction Initial from control O. £'i R number of

(%)

arve (mg)

O.8R a

i&

0-.

-.5 Comment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

Environmental Testina Solutions. Inc.

Page 3 of S PpKCICR Test Number: td o

Species: Pimep halespromelas Survival and Growth Data Day 600 mg KCUL 750 mg KCVL 900 m KCiL M

N 0 l P Q

R S

T U

V W

X O_.

11 010 1 0 I

lo ito /0 /

10 1

/0 I 1____I_____

I?

0

/0 I0

--1A 2. 12z

'z & t 416 It)

/0

/O 8

2 2.Z 3D 4 4_I_

/0

/t /D 14 8

1 (o

2-Z 4 *rio/

(

/0 5

8 1

-I a

6__

_lo lo o o 4

O 1 t b

6 ZI

'I )

g d 9"i

-I i i-j4g4 7

t8 o 10 9

4 A - Pan weight (rg)

Tray color code::

I I'.S ti. 10 1q.it q14 l.OS 1q.9s 15.06 %9..O 1II.A

143, A nalyst: - E t a14 iv 5 N.

s ol t4.

IL.. S iq t B - Pan + Larvae weight (mg)'

Analyst: :I Z

Zl0.21A 22e4 210 l1. A 18i.23 OIq.'S 1q.9_1 1.z Is2.5 Larvae weight (mg) - A - B 5.'S 1.19 8

.q4 1.2.

2.25 3.

L1 W0 4.o0

,3A Weight per Initial number l _

of larvae (mg)O O-b o

o o

o o

O av O

O

-C/ Initial number oflarvae Average Percent weight per reduction Initial from control O.oZ 2

0

.l1%

O.3176 56:1%

O.O0ZSq 1i.i~

number of

(%)

larvae (mg)

Comment codes: c = clear, d = dead, fg = fingus, k = killed, m = missing, sk sick, sm = unusually small, Ig unusually large, d&r - decanted and returned, w = wounded.

Calculations and data reviewed:

commtents:

Environmental Testing Solutions, Inc.

Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pmephalespromelas Quality Control Veriflcatlon of D2at Entry, Calculations, and Statistical Analyses Tnt amben Ppfl CICR 106 (A" o5351 Dtp SLI Taid.tess Je17-t142005 Rteywedbyi

(

(S4.

Jew*e I

-)i~.

A.-ft.

n

(. we B-f.L-L'.*eM~(J Wftk41I1n-q

64.

.M C-4Mkw d.6hsWftk.I 3"od.

N66M".d P4-.Og

.666

.a.

C~dwed.9

?..I 5-.4.56.5

_X b_

6-

_0g4td

- A.11

_Ae btud

-6.

1.

W O_"

b 1h "Wd

)

~

A 10 lo 3144 21.5o I66 0866 0366 C9,4 B

30 30 14.61 23.32 3.63 0.863 0.848 3.3 0 863 fools

M8 3.3 Ne.tapplicable C

10 30 1438 22.92 8.34 0.8o4 0 8e4 D

10 30 1482 22.89 1.07 0.107 0807 a_

10 to 14.52 23.16

.64 0864 0664 30 r

-0 30 14.93 22.51 7.38 0.75s outs 07 88 10o.o "is 43 3.6 G

10 10 14.93 22.76 7.83 0.783 0.793 30 0

14.91 23 64 8 66

- 0166 0.666 10 Io0 3.30 2291 771 0.711 0.781 450 JO 30 t0 14 54 22 82 3.28 0

0.793 4 2 0 828 loo

..7s93 4.2 Ls K

I0 10 14 6 22.73 8.10 H"!

0.1O0 L

10 _.

14.1 22.34 7.51 R733

0. 753 30 a

I 3.06 2061 3.55 0.694 0

606 14 0

3 10 14.79 23.97 7.33 0.768 0.727 7.t 0.719 9S 6.672 11.0 20.7 0

1 0 3 0 15.10 22 04 6.94 0694 0694 3

10 9

14.8 22.10 7122 0.802 0.722 t

l0 4

14.74 17.9 2.83 0.7133

.235 754 R

10 6

1463 33.23 3.S3 0597 0692 12.6 0358 55.0 0J76 19.6 iS.7 S

30 7

14.13 19.45 4.60 0657 0460 T

10 15.06 19.07 4.01 0.802 0.401 30 1

0 000 000 0.0 000 0000o 14 oo O

600 0060

~

_-o V

30 I

146S 3.25 060 060.49 30.0 05.0

.025 12e.6 97.t W

30 0

000 0o 0 00 0 OD 0000 X

I0 1483 11.22 0.39 0.390

.039 De.-We MSD Vllums PMSV3 0.6087 10.1 MSD -

Minfinm Significant Diflerce 7MSD*-

erent Minimu Sitlrcent DiffTrence PMSD i18 mtsieegurcrtofi precison0 The PMSD h fthe inimrW parent d (Terice between the cootrof and tnetenm thxt can be decisred stisa¶icaly Ognicanitin a whole emuent toxicity et On

-aqe sigoiantditreoast ortial TeS o hnieu 8ncind wxi-dlyiaeolhxielnt nduen i.ph.Ia by 4.0%

w c4(deeminedIhmsgh ftftewe tonks test intd Lower PMSD bourd deteramined by USElA (I0th percentile) - 9.4%

Upper PMSD bound detemined byUScWA (90th percentile)

S 33%.

The lower and upper nbads were calculated by the USEPA uing 205 tests conducbed rem I39 laboreatoee fot Pimephales gsrwth in Cheonic rercrence toxicantt tks USEPA. 2000. Undestnding xnd Aceeountig ror Method Vaiability In Whole Effuent Toxicity Applieieoes Under the National Pollare Dischate Eimimetion Protv.

EPA-833-R40403. US Eiemnue l Prtection Agenry, Cindnnt. OiL Oepfeni obrafeeefion Aquatic EfoSyrrew. I4c.

es 7405

Environmental Testing Solutions, Inc.

Statistical Analyses LAnVI F161 CrVWt and Sorvll TI7 Day SorYl St3tt DUC 6/7t2005 Tes ID:

PpKCt R Sample ID:

REF-RefToIJant EndDate 6/142005 Lab ID.

E-TSRakTctiag SOL SampkeI:

9C1otasischloride Sample Date:

Protocol PWCHR-EPA421-R-2-013 Test Species:

PP-'imephaes prtmoli Commentr CoacImgfl 1

2 3

4 D-Conrol 1.0000 1.0000 1.0000 1.0X0 300 0.000 1.0000 1.0000 1.0000 4kO ltXO 1.0000 1A XO

.OXO t00 Q1000 1.000 IDX0 Q90D0 750 0.4000 0600D0 0.7000 0.5X0 900 (X0000 0.1000 0.0X00 Q100 Trsasfo.ri Amh Squav Riot Rank 1.-TOd-Nombr Total COatc.mg/L Man N-Mm Mei Min MaT CV%

N Sum Criticl Rip Number to-Con3ol l.OX 1.0000 IA120 1.4120 IA120 Q000 4

0 40 300

.0=0 1.0000 1.24120 12.420 24120 Q000 4

ISQ0 10.00 0

40 450 I.=

1.0000 IA120 1.4120 IA120 0.000 4

11.00 10.00 0

40 600 Q9250 0.9250 1-951 1.1071 IA120 21347 4

14.00 10.00 3

40

750 0.5500 0.5500 0.1362 0.607 0.9912 U.736 4

10.00 10.00 Is 40

9C0 0.0500 0.05C0 0.2403 0.1503 03218 39.161 4

10.00 20.00 32 40 AxtUw.ryTmto Stt d, Ci Skme Krt Saapk-Wiks Tt kidt noal ditrbu n (p > 0.01) 09C9913242 0.1U4 032721724 0.tC4336105 Equalty etvarinse cunnot be eoaflrmed Hlypoteis Tht(1l. L.05)

NOEC WEC CbV TU Steels Many-Ome Rank Test 600 750 670.1203932 T09umentsb, DnConrol Maimnum Uikeihod-ProbWt Parameter Volvhe St 95%FtdoelUlmhz Coatrel ChLSq Crdtleut Vet1e Mu Ssma Iter Slope 2 7.22639129 2Z470121,425 12.32543951 22.06234302 0

I.639036545 7.214727703 0.65 2.372447202 0.05804277 3

Intecept

.44.433346 7.155OS564.52.4102999.30.5563922 Point Preblts m/L 95% Foad.l Luals 0i EML 2.674 546.26476 472.4191062 590.51136136 EO05 3355 5933642275 541.534013 635.201092 0.2f EC10 3.71S 622.1323355 572.001364 661.9325497 o.7 ECIS 3.964 649.0519034 603.6137767 610.5U3672 Et =0 4.151 666.11173C5 6.4527391 696.1420921 a°'

EC25 4326 6SI.2311457 642.5231534 710.125631 a.5 EC40 4.747 720.6720796 6SI.569S797 748.8651241 Ecso 5.000 745.02653 715.73746 775A421997

£C60 5.253 771.1774031 7410353099 205.003492 0.

EC75 5.674 315.1326932 703.7519175 341.2521529 02 Ea20 5..42 *34262S1I4 799.3322776 MA5.W=7762 EMS 6.036 156.27151" 3I2424216 916.1251962 0.2 EC90 6.2'2 304.7901159 U41.7694741 956396907

0. 0 2/295 6.645 921.156832 176.6494321 1020.471647 1

t0 tWo

1000 U000 EO99 7.326 1017.394711 944.1676725 11547613 Doze nv.

peas.

13.4 Organlism obtafnedffrom Aquadk S/aSysfvvms, Inc,0-70 06407405

Environmental Testing Solutions, Inc.

Statistical Analyses Lajw.2Vleb Groth sad Serinlajet.7 DayGr..lb stul Dita:

67/2Do5 Teat 1D PpKCC Sample2ID.

rFM-It T

loxent Ed DataD 6MW20 LA) M.

MEanvk.T.Tkg Sot Sample Type:

KCLhPobuiazk chldi Sample Date:

P.tocot IWCHR.EA-21-R.02-013 Tea Specics:

PP Pmnepbhks proamels Cooc"ngfL I

2 3

4 DCxmtrl 0.3660 0.3650 0.35.0 0i070 300 0.3640 0.7530 0.733 03660 450 0.7310 0.3230 111100 0.7530 600 0.3350 0.7130 0.6940 0.7220 750 0.2250 0.3330 0.4600 0.4010 900 0.OD0 0.0600 0.00c 0.

0.0390

Trrevr : Uatrat.rn."d I-Tab"d Istlok C..c.nlfL Mfta N.M".

Mea min Max CV%

N OlStt clres MSD M.

N Meta D-CootI aO310 1.O00 0.4W0 Mt70 0.3660 3236 4

0.3430 2.0000 300 aun17 0.9643 o0s173 0.7530 03660 6.73S 4

0.353 2.290 0.0657 0.11173 09643 450 0.7930 0.9353 0.7930 0.7S30 0.3230 4.1M6 4

1.469 2.290 0.OS7 0.7930 0.9351 C600 0.6723 07927 0.6723 05550 0.7220 11.717 4

4.696 2.290 0.0637 0.6723 0.7927 750 0.3760 0a34 0.3760 0.2130 0.4600 19.96 4

0.3760 0.4434 900 0.024l

.0292 0.0243 0.0000 0600 120.S54 4

0.024 0.0292 AnxihlwTests Stistic 0t1,l Stew 3.nl Shapto-wWo Test kidtet nal dotbeiOm (p > 0.02) 0.837975037 0.o44

.1.076017 0.174360589 Brutlet Test kSdbtaa equal varhnm (p 032) 3.50654t4uo I1.3446675 ItVpdbab Tat(14D 5.05)

NOEC LOEC CV TU MSD 5

MSDp B033 MSX F-Prab df Dunnte o Test 4<0 600 5l9.6152423 0.033713362 0.101074719 0.0237305 0.002101792 0o.2719523 3.12 TIeseOnts n Dlcomfro LJsar ItarplblaI (2U Rc ampr.)

7.1j3 e/L SD 95% aC XF)

SkM ICos 373.64 39.73 73.6 527.7

.0.2273 ICIo 417.02 21.39 430.22 5652 0.4737 ICIS 539.69 29.44 47130 633.30 0.2776 1.3 IC20 592.36 2533 49.33 63S.93 3.397 0.9 IC25 613.3 13.73 529.70 6S344

.13441 3C40 68276 2230 643.17 72.29

.0.1449 Og:

ICSO 725.70 14.26 686.20 77+/-33 0.0623 0.7

3.3 i 0.4 0.3 0.1 0

200 400 g0o 300 1000 Dose Mwt D.-Ravpse Pl3t

. I 60.

33]

075 as; 1e66 I

3.3 0.1:

14*3, o0.0s lo of slgficance OrgmniEu obtald from Aqtsua BioSystems. /no7 06407-0

IEnvironmenalTesting SolutionsInc.

Page 4 of S Species: Pimephalespromelas PpKCICR Test Number: _kQ Daily Chemistry:

D.

Analyst KEt 1

7fe~ AY VL ki)

~y Concentration Parameter 9

=

CONTROL pH (S.U.)

-.4+/-a5..

LkZ 3-*e

-'i8.'

DO (mg/L) -0.0 (3.

Conductivity zo'0

.6(mhos/cm) 30 0

1_

Alkalinity L

(mkg CianCtO/) _t Hardness Temperature 2CA. 8 pH (S.U.)

- 0 Tu

-4.85 -1 t-ON

.1.824 l 5,26 DO (m__

_)

4

(.5

('C) 6__

___5 5_

300 mg KCi/L Conductivity 5145 L

5e 8 10 e

([imhos/cm)

Temperature Zq9 2'4..

l 4*

pH (S.U.) e~

I4

-,1 s

.S1 1.C

nPq DO (mg/L)

.5 4.1.5 450 mg KClVL Conductivity 1090 1110 (pgmhos/cm)

NMI I

Temperature 25A 24.1

-lL A3

-l71 -I 2.

pH (S.U.)

(-C).

(,_

_1.

8_1

_1_

DO (mg/L)

(3S 3_

4LI z

?0 600 mg KCVL Conductivity I

I 4-a 0 I qt Temperature

.I l

3 24 2A4-pH (S.U.)

L J-41to 9-43

-1.(07o

-I 128 t

DO (mg/L)

__9___

750 mg KCVL Conductivity (jpmhos/cm)

M3d0 Temperature ZZ4.

z-L

-L*. 6 b

(QC )

2 q__

pHi (S.U.)

413

-4-(0

-4. 4

-. 6-k 08 9

DO (mg/L)

W

.t 5.0

.{D4

.10.

900 mg KCiL Conductivity

(;im hos~cm )

1 q 5 B o_

Temperature 2S.7 ZLI I

zq.1 2!i.2 2q.9 24.1 S T O C K_

_ ('

uIC

)

STOCKConductivity SO K(g'mhos/cm)

IO Inttial I

Final I Initial I

Final IInitial Final Initial I

Final I Initial I

FIn1 I Initial

Environmental Testing Solutions, Inc.

C ag C of S

J..

Page 5 of 5 Species: Pimephalespromelas PpKCICR Test Number: jt

!D y

^v

Q3 4

15 6 i i~l*

Uf Concentration Parameter CONTROL pH (S.U.)

tP S

.12 l_

1_.

DO (mg/L)

-4. e 3.2.

?

_____9 Conductivity 0

2g1 (Pmhos/cm)

Alkalinity (mg CaCO)L) l2\\

Hardness (mg CaCO3/L) a2.

gO Temperature zS.l z.3 Z7.5 2..L L'.4.a Z4.s

-zq.1 Z4.L.

()_

pH (S.U.)

n1.'q' 300 mg KCi/L DO (mg/L)

-4.

Conductivity I

(jimho lm) '

S116 Temperature (0C)

Z.s. Z 3.56

.1:

N

.1 24.2q T-.,,

S-4J pH (S.U.)

450 mg KCiL DO (mg/L)

Conductivity (jimh s/cm) 1170 3 II-+o

-4. Ld-+ - -

7.7i -

-zq.s 1

1

'7--7

-Lq. t

-24.51 I?.58 1.3

."NM

.In~

-j.0 2I-4 I

fw----

dW A

.24'i 7.,F<

.74 24.9

_. I n

Temperature

( 0C)

ZS.?2 2q.9 24LS.

pH (S.U.)

_._'.oS_

3-.

.(ol

-4TB

. 4'S 600 mg KCi/L DO (mg/L)

I L

1.

Conductivity MOO (jimhos/cm)

Moo__

Temperature

-s.L 2.4.1 7.q 2i

-Zq -i

z. J R.

(0c )

pH (S.U.)

8.oS40

,S5t Z4-0 O ?

5 DO (mg/L) 113 750 mg KC1/L Conductivity 15r0 Temperature Z5. l

-IS 2 l.A 4.3 ILa.8 4.-.

zq.l J pH (S.U.)

8.OL.

_. _ 4.0__

+.

.4qZ S-v-1.3 1 7 Gg DO (mg/L) 0.0

-4.6 0

9.9 l.

900 mg KCVL (Conunctmi)ty 1/?20 2_oo_

Temperature 2.S.O NAl

. l-9 7!q.

WlSR 2q.

Z B

STOCK Conductivity

__________(ptmhos/cm)

Initial Final IInitial I Final I

WHOia I

Final I Initial I

Final I

L

.1.

J

Environmental Testing Solutions, Inc.

Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 1.12 1.10 1.08 1.06 1.04 USEPA Control Limits (I 2 Standard Deviations)

~ ~~.........

c:

I-4 U

2.5 2.0 1.5 1.0 0.5 USEPA Warning and Control Limits (75th and 9ceh Percentile CVs) 1.4 1.3 1.2 1.1 1.0 0.9 0.R Laboratory Warning and Control Limits (l0 h and 25th Percentile CVs)

.S A10, 01"-

%'

t%

t

0 Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

Central Tendency (mean IC25)

Warning Limits (mean IC25 E SA.X0 or SA75)

. Control Limits (mean IC2 4 SA 25 A90' or 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Sodium Chloride Chronic Reference Toxicant Control Chart

'for Cerlodaphnla dubla using Moderately Hard Synthetic Water Test nunber Teat date 7-y ICS, CT (gQ NIIC)

(g/LN CI)

State and USEPA Labortory S

Ceral LMnMs S,s W.Waning Lnits S&2 5 CT-2S CT+2S CT-SA,11 CT+SA.14 Labentery Centra Lnits SAJI CT - SAII CT + SAM USEPA Waning Llidts Sit Cr - SAT CT + S.A5 USEPA CeOf MULlnits CT - S Cr + SA.

CV 1

2 3

4 5

6 7.

8 9

10 11 12 13 14 15 16 17 18 19 20 1244-03 01-06.04 02-03-04 03403-04 04-06-04 05-04-04 06-08-04 07.07-04 08-10-04 09-14-04 09-14-04 10-05-04 11-02-04 12-07-04 01-11-05 02-08-05 03-01405 0405-05 05-03-05 0607-05 1.08 1.06 1.06 1.07 1.07 1.07 1.06 1.06 1.06 1.05 1.07 1.07 1.06 1.0O 1.06 1.05 1.08 1.09 1.07 1.05 1.07 0.01 1.04 1.10 0.09 0.98 1.07 0.01 1.05 1.09 0.09 0.98 1.07 0.01 1.05 1.09 0.09 0.9S 1.07 0.01 1.05 1.09 0.09 0.98 1.07 0.01 1.05 1.08 0.09 0.98 1.07 0.01 1.05 1.08 0.09 0.98 1.07 0.01 1.05 1.08 0.09 0.9S 1.07 0.01 1.05 1.08 0.09 0.98 1.06 0.01 1.05 1.08 0.09 0.98 1.06 0.01 1.05 1.08 0.09 0.98 1.06 0.01 1.05 1.08 0.09 0.98 1.06 0.01 1.05 1.08 0.09 0.98 1.07 0.01 1.05 1.09 0.09 0.98 1.07 0.01 1.05 1.08 0.09 0.98 1.06 0.01 1.04 1.08 0.09 0.9S 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.18 0.89 1.15 0.1 0.89 1.15 0.18 0.8S 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18 0.88 1.15 0.18

0.8S 1.15 0.1 0.89 1.15 0.18 0.89 1.15 0.18 0.88 1.25 0,48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.24 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.25-0.48 0.59 1.25 0.48 0.59 1.25 0.48 0.59 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 OAI 1.55

.0.66 0.41 1.55 0.66 0.41 1.54 0.66 0.40 1.54 0.66 0.40 1.54 0.66 0.40 1.54 0.66 0.40 1.54 0.66 0.40 1.55 0.66 0.40 1.54 0.66 OAO 1.54 0.66 0.40 1.54 0.66 OA0 1.55 0.66 0.41 1.55 0.66 0.41 1.55 0.66 0.40 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 172 0.01 1.72 0.01 1.73 0.01.

1.72 0.01 1.73 0.01 1.73 0.01 1.72 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 Note:

I-d IC,, day 25% inlbitiou concentration. An stimaton of the concentration of sodium cbloeide that Ymuld caune a 25% reduction in Cerfodaphnfa reproduction for the test population.

CT - Central tendency (-n lCl).

S - Standard deviation of The nC value, Labenatexy Ceral ansd Wantin LUnits Labontory contl and wrninglimitswere etblisd using he standard deviation of th C2, values correqinding to te 102thand 25thperentile CVL Tese ranges ro more stingent than te contl and wrning limitb reconmmended by USEPA for the test method and cndpoint.

SAl, - Standard deviation eoneapouidIng to the 10" percentile CV. (SAs - 0.08)

S.29-Standard deviation conesponding to tho 25*percentile CV. (S,

-0.17)

USE1'A Cenral and Waning Llnits SA7 - Standard deviation eorresponding to the 75' perentile CV. (SA, - 0.45)

Su - Standard deviation corresponding to the 9go percenble.CV. (SA" - 0. 6 2)

CV-Coefficientofvariationofthe Cn values.

USEPA 2000. Undetanding nd Accounting forMethodVariability in Whol EfMuent TosicityApplicstions Under the Ntional Pollutant Discharge Uimination Progm. EPA.S33-R-40-003. US lnvinmentl Protection Agency. Cincimati, OIL

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Tet T

est date Control Control Mean number Survival Reproduction CT CV CT MSD PMSD CT for Control Mean (4/)

(opyring/fernale)

Reproduction (offmprinVferale) for Control

(%/)

Reproduction CV (%.)

(%)

for PMSD (%)

1 12-04-03 100 2

01-06-04 100 3

02403404 100 4

03-03-04 100 5

04-06-04 100 6

05-04-04 100 7

06-08-04 100 8

07-07-04 100 9

08-10-04 100 10 09-14-04 100 11 09-14-04 100 12 10-05-04 100 13 11-02-04 100 14 12-07-04 100 15 01-11-05 100 16 02-08-05 100 17 03-01-05 100 18 04-05-05.

100 19 05-03-05 100 20 06-07-05 100 30.4 30.6 30.7 29.0 28.1 32.5 32.9 33.3 27.4 28.7 28.8 29.6 30.5 31.8 31.0 30.0 29.9 32.4 29.9 30.4 30.5 30.6 30.2 29.8 30.2 30.6 30.9 30.5 30.4 30.2 30.2 30.2 30.3 30.4 30.3 30.3 30.4 30.4 30.4 9.7 4.4 7.2 7.8 6.2 6.4 6.8 6.3 5.8 4.9 7.5 8.2 8.5 4.9 9.4 6.8 6.6 6.9 7.0 5.0 7.1 7.1 7.3 7.1 6.9 6.9 6.8 6.7 6.5 6.6 6.8 6.9 6.7 6.9 6.9 6.9 6.9 6.9 6.8 3.6 11.8 3.5 11.5 2.7 8.8 3.0 10.3 2.8 10.0 3.3 10.1 3.0 9.0 2.6 7.8 2.3 8.6 2.4 8.2 3.0 10.5 2.8 9.6 2.7 8.8 2.5 7.9 3.1 10.0 2.4 8.0 2.9 9.9 3.1 9.6 2.9 9.8 2.6 8.5 11.6 10.7 10.6 10.5 10.4 10.2 9.9 9.8 9.6 9.7 9.7 9.6 9.5 9.5 9.4 9.5 9.5 9.5 9.4 Note:

CV-Coefficient of variation for control reproduction On iverage, the CV for control reproduction is 6.8% in Environmental Testing Solutons, Inc. Ceriodap;hnia chronic toxidcity tests.

Lower CV bound detenrined by USEPA (10' percentile) - S9%

Upper CV bound deternined by USEPA (90' percentle) - 42%

MSD -

Kinimum Significant Difference PMSD - Pecent Minimum Significant Difference PMSD is a mnesurc oftestprecision. Thc PMSD is thc minimum percent difference between the control and treahfnnt that can be declared statistically significant in a whole effluent toxicity test On avrage, a significant differCce occurs for Environmental Testing Solutions, IC. chronic toxidcity tests when a toxicant reduces Ceriodaphnia reproduction by 9A% fom the controL Lower PMSD bound determined byUSEPA(10 percen6ile) -1 1%/

Upper PMSD bound deternined by USEPA (90' percentle) - 37%.

CT - Cenl Tendancy (Mean Control Reproduction, CV, orPMSD)

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodap~hnia reproduction in chronic Tefeten=c toxicant tests USEPA 2000. Understanding and Accounting for Method Variability in Whole Efuent Toxicity Applications Under the Natonal Pollutnt Discharge Eimi0natin Program EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati, OIL 06-07-OS

Environmental Testing Solutions, Inc.

Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 35- _-

E30 X:; lb 25............

25

° 320 U

15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female)

I I

I I.

$ 40 North Carolina Acceptance Limit (< 40.0%)

<°30 I-s Kentucky Acceptance Limit (< 30.0%)

o a 20 U 10 00 40 I

I I

I LI i

i USEPA Upper PMSD Bound (90 percentile < 37.0%)

30 20 10_

0.

I I

I I d I

Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Reproduction, CV, or PMSD)

Control Limits (mean Control Reproduction, CV, or PMSD 4 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Page I of 6 Environmental Testing Solutions, Inc.

Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia CdNaCLCR #:

36 Dilution preparation information:

Comments:

NaCI C-M numbert Stock preparation:

100 g NaCUI (dissolve S0 g NaCI in 300 ml deionized water)

Dilution prep (mtL) 600 S00 1000 1200 1400 Stock volume (ml.)

9 12 1 5 1 8 21 Diluent volume ImL) 1491 1488 1485 1482 1479 Total volume (l.)

1500 1500 1500 1500 1500 Test organism information:

Test information:

Organism age:

< 24-hours old Randomizing template:

Date and times organisms C S.OI.O5 lS$z.9 nlto tITO.

Incubator number and were born between:

__shelf location:

Organism source:

Os,%-OS r

A tA YCT batch:

6 lI~cS Transfer bowl information:

pH =1.AA SU Temperature =ZAX C

Selenastrum batch:

CfS-j90S Daily renewal information:

Day Date Test Initiation, NIHS water Analyst renewal, or batch used termination time 0 *

(

soT-OS 9

o'.-o4-5A I

6oOt -es SA 2 2

Ctoc.S 04 s.-c-OS C 3

O'ii-c0s C-4____

6,.-rL-CiE ii.

o06-0os A 5

03 s

6 13 OL 6

6 CarV&-QS:

11Z 1 Z

7 Ob u14-cs I oil1 Control information:

Acceptance criteria Summary of test endpoints:

% of Male Adults:

4 O7.

s 20%

7-day LC50 7 lzlo

% Adults having 3' Broods:

p00%

a 80%

NOEC fto

% Mortality:

O.

s20%

LOEC

/000 Mean OffspringTFemale:

1 15.0 offspringfemale ChV t'

I%_CV:

I S0.

-o

< 40.0 %

C25 1 oO. 5d,

I : Environmental Testing Solutions, Inc.

Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR N:

3Va CONTROL Survival and Reproduction Data Repiicate number Day 1

2l 3

4 5

6 7

-8 9 l

1 Young produced O

0 1C 1

0 101tlOlC lC Adult mortality U

U U

l L

l T1 2

Young produced l

I.

C} j.

loT 1__

Adult mortality J l

U U

U t

l U

U.

3 1 Young produced O

l I l I

l Adult mortality j#H1 lL 1

l I

1 Ll L

l 4

Young produced l

5 14h 5

s 3

IL sIs Adult mortality L-1 1

J1.I S

l Youngproduced i l l l

I '

'1lI2.lI 0

l II l I llC) l Adult mortality j

U L-I.

6 Young produced Ql I 10 O

1.Q....l1c IOO___

Adult mortality l-L-

'-1 L-7 7

Young produced I co

'L S

I 5

I I I

Total young produced 931

.C z' ll 33 I

3

z.

30 z8 Final Adult Nortality t

e. f C t r

7 -I X fr Yd Broods C

>ClCl Cl Cl ClC C

Note: Adult mortality (L - live, D i-dead)

I 600 mg NaCL/L 4 cp&M ott.

Concentration:

% Mortality:

I 07 Mean Offspring/Female:

I 3o.-LA Survival and Reproduction Data l

l Replicate number Day I_

I.

2 3

14 6

177 8

1 9

I 10 1

Young produced l

D.I.I O II O 101 o

I

. I o

LI I

Adult mortality l

U.-.

lL l

[

l T

U L-L-..

r2 lYoung produced1

)

O Ol Cr c

To c IoIo I

Adultmortlity

-3 Young produced l

l I(

Adult mortality l

L-L--

L -

UI 4

Youngproduced lC 04 4A

'4 5

l l

Sl l

lAdult mort llty L-l>l>ll>l>lltL-u s

Young produced l-

-1 Adult mortality 1

l_.

I I

J L4 I.

UI-l 1

6 Young produced l "

l Adult mortality l

U-.

l l_

l i

l U.

l

__lll l

1 U-7 Young produced is 1

l jj l 1 I

Total young produced l 3z 33 l31 3

l 3Z T33 l 3 T2 32 l 31 l 3Z Final Adult mlortality j L..

L U

U Ll U

l I

l l

l iksetlr I

60P:)

Note: Adult mortality (L - livc, D - dead)

I Concentration:

%Mortality:

0 7o l Mean Offspring/emale:

I el

% Reduction from Control: l-'1.'i

liEnvironmental Testiing Solutions, Inc.

I Eo

-Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR M:

3t I

I

.I 1.

800 me NaCI/L Survtival and Reproduction Data U.

I I

.II I..

II' I

k.1, S.

, __=

Replicate number Div t

2 l3 14 5

l 6

7 8

9 to I

Young produced 1 C I 4

C 8

O 10 Adult mortality L_

L L

L.

L t

2 Young produced o

C)

C0 l Q

O l C i 0 Adult mortality j L

1 l

L L

t 3

Young produced L

IJ CL

.C

.C C. )I

°Q 0

C C

O Adult mortality L

L L

I I

I

\\

s Young produced 5 5 I

Adult mortality lt l

l 1

l _L l

Ll S

Young produeed lj) I n l 1 I I J

1 I

.I*.I I.

I 1 T

Adult mortality 1-1_

U-U--

L.

1 Li L r 7

Young produced l05Q**LO l I (oŽ 1

0 L.

Total young produced lZ 8 30 31 z

l5 31 Final Adult lNtortalltv _

_l>l>l' Note: Adult mortality (L live, D d ad)

e

.j l Concentration:

'I F

nM ortality:

A l o-.

l Mean OffspringtFemale:

l 25i l

% Reduction from Control:

2 Z..

. l 1000 mg NaCVIL Survival and Reproduction Data IReplicae number Day

_2 3

4 5

6 7

8.

9 to l

A dult m ortality l l

?

U l

C l

l 2

1Young produced 2C J Adult mortality.

U § L

. j l.

3 Young produced l Q.

l

.0 l0 Adult mortality l L-l LlLlU 4

Young produced AI ll 1

J 5

L-Adult mortality L -

k.-

L-U L I U

U.

U L-IL.

s Young produced 19 IC0 10 I-Adult mortality T

L l-L...l

... 4 U.

Adult mortality Young produced a

1-2 I

0 2

12.C.I LI I

I x

T U.

U.

T.

U.

Total youngproduced l a2 lZ l 2.

l 3b ?- 6 lZS 21 21 l

(

30 Final Adult Mortality l

1

i.

1-C tI L.

t llL t iJT 604b Note: Adult nortality (L - live, D - dead) r Concentration:

%Mortality-6 i Mean Offspringfspnale:

21. 0

% Reduction frozn Control:

t1. 7-^ o

.Enviri I '

I'Ia-'

I...

..I.

I.

II.

I.

mnmental Testing Solutions, Inc.

Species: Ceriodaphnia dubia 1200 m; NaCI/L Page 4 of 6 CdNaCLCR #: ;

Survival and Reproduction Data

-Replicate number Day 1

l 2

3 4 1 5 6 l 7

l 9

10 I

Young produced 0

Q I

C C

o I o l

Adult mortality 1

l >

l L

2 Young produced

-o I

O

=

X O

Adult mortality l

L 1 j l

3lYoung producedf C> [

la1 O

n zo Adult mortality L-L-

L L

I 4

Young produced Z

3 Z

.l3 JZ3 Adult mortality L-.

s-_

Sl Young produced l5l lC:

O lAA l^

4§(

Adult mortality L

L-1L>1 L-U iL l

6 lYoung produced lC)

C)

I O l

)

Il>l°lO l

0 Adultmortality L-L-

L-l L

Llt-L-

(-

7 Young produced l, l

1 I

l o il I

...Q..

o L. I o Totalyoungproduced l 17 l9z l 18 l

i l3 l.

13 l9 Final Adult lortality t 1 l I

l I

l ll Note: Adultmortality (L-liveD-dead)

Concentration:

l l% Mortality:

07.

l Mean Offspring/Female:

l

-o l

% Reduction from Control: la.

4,fl 1400 mg NaCV/L Survival and Reproduction Data I

a

_ f 1456 1

I

______Replicate number 1

Young produced C

C

0.

0 G

° IC° I

Adult mortality 1

C

]

l

_I 2i Young produced o

cL o

C:>

CD C

O n

lC C) l()

l lAdult mortality ll L-l L.

lL-tl L

1 1

t 3

Young produced

[C)

[

I lO Adult mortality l

U l-l U.I-I 1

L 4

Youngproduced l-3 1 3 l

I O l zL 2- -LZ-I Adult mortality l

1 L

l Ul L-l UUIUIL-F5 lYoung produced lb 3

I IlIO IO I

I4 l2-l Adult mortal-ty C

C Ll C

L-L l.-

6 Young produced i

i O

L Adult mortality lL 1

L-L 7

Young produced (P)=jo Totalyoungproduced l

IC IJO 5

l l o

13 I Z Final Adult Nlortality L

i V

l l

I l

1l Note: Adult mortality (L - live, D - dead)

Concentration:

% Mortality:

I s7 Mean Offspring/Female:

9.8 I % Reduction from Control: j 61*.

l7o

Environmental Testing Solutions, Inc.

Verification of Ceriodaphnia Reproduction Totals Control Day Reeplilcte number Total I

2 1 3 1 4 5 1 6 1 7 8 1 9 110 1

O O

0 O O

O 0

2 0

0 0

3 0

0 0

4 4

6 4

5 5

5 3

4 5

5 46 5

11 0

10 10 9

12 11 11 9

10 93 6

0 10 0

0 0

0 13 24 7

16 14 15 17 17 15 17 14 16 0

141 Total 31 30 29 32 31 33 31 29 30 28 304 600 mg NaC/L Day Replicate number Total 1

2 1 3 1 4 5

6 1 7 1 8 I 9 1 0 1

0 0

0 1 0 0

2 o

o o

0o 0

0 0

0 3

0 0

0 4

4 5

5 5

4 4

4 r6 46 5

10 1 1 10 1 111 12 10 13 9

1 1 108 6

0 0

3 0

3 7

18 17 18 16 16 18 15 15 15 162 Total 32 33 32 30 32 33 32 32 31 32 319 800 mg NaCI/L Day Re picate number Total 1 l2 l 3 4

5 6 l 7

8 1 9 l 10I I

0 0

0 2

0 0

0 3

0 0

0 4

5 4

4 4

4 5

4 4

43 5

10 12 10 II 12 10 10 9

_1 11 106 6

0 0

2 0

0 0

2 7

13 14 17 14 15 16 16 14 14 13 146 Total 28 30 31 29 33 31

30.

28 29 2#1 297 1000 mg NaCI/L Dy iRepllcate number T tal Dy I1 2

3 4

5 6

7 1 8 Tt9 1

1 0

0 0

0 O

o 0

2 0

0 0

3 0

0 0

4 4

3 4

5 4

4 3

4 38 5

9 10 10 11 0

9 0

12 10 12 83 6

0 0

10 0

10I 0 0

0 20 7

13 12 14 14 12 12 14H1 11 13 14 129 Total 26 25 28 30 26 25 271 27 26 30 270 1200 mg NaCI/L Repcate number Total y

1 2

3 4

S 6 1 7 1 8 1 9 1 10 1

0 0

0 2

0 0

0

°

° o

o 0

0 3

0 0

° 0 0

0 0

0 0 0 0

4 4

2 3

2 1I 3

2 3

25 5

7 5

0 6

0 0

4 3

4 6

35 6

0 0

10 0

6 6

0 0

22 7

6 8

0 4

7 7

7 44 T'otal 171 121 21 8

1 1 16 131 6

113 19 126 1400 mg NaCI/L Day Replicate number T Total l

Y lI1 2

3 4

5 6 1 7 8

9 I lO I

0 0

0 2

0 0

0 3

0 0

0° 0

0 4

3 3

1 0

2 1

2 2

2 0

16 5

6 3

4 3

1 0

0 5

4 2

28 6

0 0

0 7

0 0

7 7

6 1 41 5 1 6 5

2 4

6 6

3 47 Total 15 10I 10I 9 8

10 6

13 12 S

98

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphnta dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:

CdNxCiCR #65 (#36 at 351 Depot St.)

Test dates:

June 07.14. 2005 Revelwed by: f Concentration (mgfL NaCI)

Rtevilexte number I

2 1

3 I

i 1

5 6

i i

Survival Average reproduction cI m I rents atrdw a

(%)

(offsprint/rtmale)

I

,,td.ft (%)

.lt t%)

7 S

9 10 Control 31 30 29 32 31 33 31 29 30 28 100 30.4 5.0 Not appUcable 600 32 33 32 30 32 33 32 32 31 32 100 31.9 2.7

.49 800 28 30 31 29 33 31 30 28 29 28 100 29.7 5.5 2.3 1000 26 25 28 30 26 25 27 27 26 30 100 27.0 6.8 1.2 1200 17 12 21 S

1 I 16 13 6

13 9

100 12.6 35.7 58.6 1400 is 10 10 9

8 10 6

13 12 5

100 9.8 31.1 67.8 Dunnett's MSD value:

2.594 PMSD:

8.5 MSD -

Minimum Significant Difference PMSD -

Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significantmina wholeeemuent toxicitytesL On average a significant differenceoccurs ror nvironrnentalTesdng Solutions Ine chronic toxicitytests when a toxicant reduces Crfodaphnla reproduction by 9.4% from the control.

Lower PMSD bound determined by USEPA (2 0* percentile) - I1%.

Upper PMSD bound determined by USEPA (90' percenrtile) - 37%.

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnfa reproduction In chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability In Whole Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. l:PA-833-R-00-003. US Environmental Protection Agency, Cincinnatid OIL

l Environmental Testing Solutions, Inc.

Statistical Analyses Cartedepba Servival and Repredectlen T et-Relemdetl.a Srt Date:

6'7i2005 Teat la.

CdNaCR Sample 2D REF-RefTaxicad Ead Da":

6214ZO05 Lab ID-.

ETS-Envr. Testig So Sampla Type:

NACL-SOdium chktd Satple Dater:

Protocot pWCIHR-SPA-21-R-02.013 Test Speces:

CDC aiDdqhria dubi Con Cost-mIl, 1

2 3

4 5

7 Jo D-Contol 31.000 30.000 29.000 32.000 31.000 33.000 31.000 29.000 30.000 21.000 600 32.000 33.000 32000 30.000 32.000 33.000 32.000.

32000 31.000 32000 300 23.000 30.000 31.000 29.000 33.000 31.000 30.000 20.000 29.000 28.000 1000 26.000 25.000 2t.000 30.000 26.000 25.000 27.000 27.000 26.000 30.000 1200 17.000 12.000 21.000 000 12.000 16.000 13.000 6.000 13.000 9.000 1400 15.000 10.000 10.000 9.000 3.000 10.000 6.000 13.000 12.000 5.000 Tmnssadn: Uetnmatermed Come-mgL M"ea N-Meae Mea Mih Max CV%

N D-conrol 30.400 1.0000 30.400 2t.000 33.000 4.952 10 600 31-900 1.0493 31.900 30.000 33.000 2.745 20

00 29.700 0.970 29.700 23.000 33.000 5.510 20 11000 27.000 0.2012 27.000 25.000 30.000 6.762 10

1200 12.600 0.4145 12.600 6.000 22.000 35.729 20

1400 9.800

. 0.3224 9.100 5.000 23.000 31.100 10 Rtak 1-Talled Sam Critlecl 135.50 75.00 91.00 75.00 63.50 75.00 55.00 75.00 55.00 75.00 Isetonle Mai N-Mean 31.130 1.0000 31.150 1.0000 29.700 0.9535 27.000 0.2660 12.600 0.4045 9.300 0.3 146

)

Aditary Teals statlitke Criticsl Skew Karl KoknogarovDT dicat eaoaldebutn (p -0.01) 1.06454403 1.005 0.43279813 2293S461 Bartle Teat iedktt unequal varnces (p - 4.52S45) 27.51731S7 15.0862722 HOt'els Teet(l4-WL S.05)

NOFC LOEC Ctv TV Steers Mamy.r Rank Teat S00 1000

94.421191 T

tb D-Cont. l Uear Inlerpolatle (203 R..amplea)

Nlbt mi/L SD S9%

CL Skew IOD 307.962963 31.7956531 73.13623 876.674107 0.164J ICI0 923.333333 33.7801856 S74.543t97 1001.53213 0.5761

.ICIS 1007.25694 1I.5625491 976.104013 1023.5413

-1.0142 1.0 1C20 1028.51939 7.57723699 1016.7497 1045.21699 0.3237 O.S IQ5 1050.52083 7.79412647 2038.17121 1067.51319 0.3738 0.8 2C40 1115.41667 11.1700119 1097.73342 2131.2662 0.4316 IC50 1152.68056 14.7056093 1137.47024 1119.75456 0.5602 0.0 500 1000 1500 Done mglL.

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Environmental Testing Solutions, Inc.

Statistical Analyses

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Cerlodaphale Survival and Reproduction Tedt-Reprdsetlon SWm Date:

6/712005 Test ID:

CdNeCICR Sample ID:

REF-RefToxicant End Date:

6n14/2005 LAbID:

ETS-Envir. Testing Sa Sample Type:

NACL-Sodiimnewide Saunple Dote:

PMtocol:

FWCHU-EPA-821-R-02413 Test Species:

CD-Ceriodaphniadubia Cwunents:

COne-ni-L 1

2 3

4 5

6 7

S 9

1 0 D

lCallm 31.000 30.000 29.000 32000 31.000 33.000 31.000 29.000 30.000 28.000 600 32.000 33.000 32000 30.000 32000 33.000 32000 32000 31.000 32000 800 2S.000 30.000 31.000 29.000 33.000 31.000 30.000 28.000 29.000 2S.000 1000 26.000 25.000 28.000 30.000 26.000 25.000 27.000 27.000 26.000 30.000 1200 17.000 3Z0C0 21.000 8.000 11.000 16.000 13.000 6.000 13.000 9.000 1400 15.000 10.000 10.000 9.000 t.000 10.000 6.000 13.000 12000 5.000 Transrorn: Untrunstormed I-TalIed Cone-mgiL Mean N-Mean Mean Mli Mao CV%

N t-Stat Critical MSD DCaml 30.400 1.0000 30.400 ZS.0C0 33.000 4.952 10 600 31.900 1.0493 31.900 30.000 33.000 Z745 10

-1.322 2237 Z594 t00 29.700 0.9770 29.700 2S.000 33.000 5.510 10 0.617 ZZS7 Z594

1000 27.000 0.8S82 27.000 25.000 30.000 6.762 10 Z997 Z227 2594

12D0 32600 0.4145 12600 6.000 21.000 35.729 10 15.692 227 2594

1400 9.800 0.3224 9.800 5.000 15.000 31.100 10 18.161 2.287 2594 AullliaryTests Stastile Critical Sksew Kurt KclvmogarvD Test kidiea tenncsialidstrnbuton (p <0.01) 1.06454408 1.035 0.43279813 Z2938461 Bartet TeutIndirtesaunequalvaianees(p-4.52S.05) 27.5173187

.15.0862722 Hypetheds Test 1-11,0.05)

NOEC LOEC ChV TU MSDn.

MSDp MSB MSE F-nob df Dtnnetra Test 0OO 1000 894.427191 Z59379638 0.08532225 950.666667 6.43333333 3.2B-30 5.54 Treatments vs D-Cautrol

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06-07-05

Environmental Testing Solutions, Inc.

Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:

a' Daily Chemistry:

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Analyst Concentration IParameter M

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Environmental Testing Solutions, Inc.

Page 6 of6 Environmental Testing Solutions, Inc.

Page6o6 Species: Ceriodaphnia dubia CdNaCLCR Xie: k3o

. 45 34 5

6

.Analyst Y*=

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PERMITTEE NAME/ADDRESS (Indude Facilitv Namne/ocation if Different)

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VA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 (INTEROFFICE B-SODDY - DAISY T 37384 Fcity _

A - SEQUOYAH NUCLEARPANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

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OFFICER OR AUTHORIZED AGENT AREAj NUMBER YEAR MO DAY TYPED OR PRINTED CODE_________________________

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PERMITTEE NAMEIADDRESS (Include FacilitYName/location ifDifferent)

Name TVA - SEQUOYAH NUCLEAR PLANT _

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_LINTERO9q1E aB-2@ffi__-

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OFFICER OR AUTHORIZED AGENT.

AREA I NUMB3ER YEAR MO DAY TYPED OR PRINTED I____________________________

________________jCODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference aal ttachments here)

Operations performs visual Inspections for floating debris and oil and grease during all backwashes.

EPA Form 3320-1 (REV 3199)

Previous editions may be used Page 1 of I

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000

____QE 0

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SODDY - DAISY TN 37384 aciity_ TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

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W NAME/TITLE PRINCIPAL EXECUTiVE OFFICER I Ceatily under penaity of law that this document and all attacihments were prepared wider my D/

2. i TELEPHONE DATE Jirectionor superisionInaccrdancwithas ytem deslgned'toassurethatqualifiedpersonnel 44 1.f^

OI" J. Randy Douet propedy gatherandevaluate the Information aubnstted Based onmy Inquiry ofthle erson or_ w Si. Vie reeen iimon. the Informathon subtm itote hIos tothe best of my knwedge and belief true Prindpa Eniomntai Engineer 423 843-6700 05 l07 l14 S Ite VicePresidentaccurate, and coniplete. a m aware that there are significant p enalties for aubmnsttngi false SIGNATURE OF PRINCIPAL EXECUTiVE TYPED____

OR__PRINTED

_____nformalion, including the posibility of fine and lngntsonment for knowing violations.

OFFICER OR AUTHORIZED AGENT AREA NUBE YEAR lMO lDAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Operations performs visual Inspections for fioating debris and oil and grease during all backwashes.

EAFr3301RE3/9 PrvoseItionsmaybnte usedmaio Pagete 1.totebetofm of 1dean ele. re EPA Form 3320-1 (REV 3199)

Previous editions may be used Page I of 1

PERMITTEE NAMEIADDRESS (indude FecItv Neme/Location if Different)

Name _TA

- SEQUOYAH NUCLEAR PLANT ____

Address P.O. BOX 2000

__ _ _ffjLEaOfflE _ 2_

_ _ _ _SODDY D2AISY_

TN 37384 -

aciitv_y VA-SEQUOYAH NUCLEAR PLANT Locaton HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

(SUBR 01) 0MB No. 2040-0004 1

TN0026450 118 G l F-FINAL I

PERMIT NUMBER I I DISCHARGE NUMBER WASTEWATER & STORM WATER FmNIToRING PERIOD I

YEAR I MO I DAY~

I YEAR 7IOl A

From 05 l 06 l 01 I To 105 l 064 301 EFFLUENT NO DISCHARGE [ 1

NOTE: Read instruclions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

FREQUENCY SAMPLE

_EX__

O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)

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WEE SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1

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.~L~t-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

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TELEPHONE DATE direction orsupervisionIn accordance Will asysterndesigned to assure that qualifiedpesne W

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J. Randy Douet property gather and evaluate the Inforriation submitted. Based on my Inquiry of the Person or T

persons wto ebnage the syster% or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 05 07 14 Site Vice President i~nformation, the Information submitted Is, to the best of my knowledge and befief. true.

Ste Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTED infomnation. Incuding the possibilityof fine and Imprisonment for knowing violations.

OFFICER OR AUTHORiZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED It CODEL COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference atl attachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EP Fo_

33_- (RE 3_9 v

edti. m.b use Pag 1. of 1 EPA Form 3320-1 (REV 3199)

Pravtous editions maybe used Page I of I

ML051990397

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ML051990397

Text

Dear Mr. Hannah:

SUMMARY

Contact:

Contact:

Contact:

SUMMARY

SUMMARY

SUMMARY

SUMMARY

SUMMARY

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