ML051990397
| ML051990397 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/14/2005 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML051990397 (116) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 July 14, 2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6"' Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JUNE 2005 Enclosed is the June 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
./II 35 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pfted on cyc~e W
PERMITTEE NAMEJADDRESS (Include Facilvit NamoA ocat on ifDiffeent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ____________
_ _ _ _LPEIROMIICE S-0 I TNO026450 J 101 G I F-FINAL SODDY - DAISY TN 37 84 _______ I PERMIT NUMBER I DISCHARGENUMBER DIFFUSER DISCHARGE Facility VA- SEQUOYAH NUCLEAR PLANT_ -_ -
Location AMILTON COUNTY MONITORING PERIOD I EFFLUENT ATTN: Stephanie A. Howard From --- 0 To l .. NO DISCHARGE Z ***
F6 I0 NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
___EX__ __ _ __ _ __ _ __ _ __ _ __O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS E iT TEMPERATURE, WATER DEG. SAMPLE 28.2 04 0 30/30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 DEGR.Cz..****SEE- CK***
REQ ;+~.
INSTREAM MONITORING :'REQIIREMENT. ...
-' '. *'^.i ! i : ., *DAILY. MX , __ * -' E TEMPERATURE, WATER DEG. EASMPLE 40.6 04 0 30D30 RCORDR CENTIGRADE MEASUREMENT 00010 1PH 0 0 ~~~~~~~SAMPLE M.P--pERMrT,**** **** , ****74O ____-__-__4.
- 0; 03 110 RCOR GA R EFFLUENT GROSS VALUE . ;RE,`DUIEMEN-
.... ,. IMUM - .. . T CR MINM PH T SSAMPLE ***** ** * . 5 8 19 0 PM/30 GRAB MEASUREMENT 7.4 12 00400 1 0 0 ;-6.0 * >******; ,9. '.
- UpERMTŽl-
- . WEEKLY GRAB;-
EFFLUENT GROSS VALUE REQUIREMENT...M... iMAXIMUM,-- 7.7 DiX SOLIDS, TOTAL SUSPENDED SAMPLE 5 8 19 0 5/30 GRAB MEASUREMENT . 19 EFFLUENT MEASUREMENT EFFLUENT GROSS GROSS VALUE VALUE - EEQUIREMENT .i s4 :- M ..
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- DA DAILY Y-MX~ - ___ MG__-_EEKY FLOW IND 50056 500530 1 0 0 1CONUITORSE 0 R A-REUIRE SAMPLE15800 1'DEI~ or MGET ;Y:,- 3030
-WEEKLY MGRAB,<;1--.- GRA CRD OO-ROD TETETPATMEASUREMENT < 51 /0 GA 500506 OILOADRIEOAS 1 0 0 ESDA , PERMIT,:-"-_-.'.
SAMPLE *******
REOT MDW~~
- 5
- OTNROD
- 051.3 19 0 50/30 GRAB CHORN,OTLREIDA SMPE0.1 1 M.03 0 E30
/ :0,GRAB MEASUREMENT, EFFLUENT GROSS VALUE ~ EURMN .IS A ___
TYPED1 OR PRNE MGD___________________________TI________________R NAME/TITLE PRINCIPAL EXECUTiVE OFFICER I Certif unter penalty of law at tis document and al attachrent were prepared under my TELEPHONE DATE J. Randy Douet ~properly gather and evaluate the Informaetion submtted. Based on mnyinquiry of the person or iJ tC persons who manage the Syte.orirleithose peson irectyirespoansiblie forgthrung Pl al Environmena 23 84iee 13-6700 05 07 1 Site Vice President accurate, and cornplete. lam aware that there are significant penalties for suomitting false SIGNATlURE OF PRINCIPAL EXECUTiVE ......... L...................1 TREATMENTPMnfoAmation Incuding the possibiityof fine and IRonentforknowingvioons. OFFICER OR AUTHORIZED AGENT ADEA NUM8ER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. CCW data for June 2005 Is attached. Veliger monitoring Infomation Is attached.
A m 31 (V . P . s en m e ud P o EPA Form 3320-1 (REV 3199) Pirevious editions may be used Page I of 2
CCW TRENCH DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DatelTime Analyst Method 06/03/2005 0950 <0.5 mg/L 06/07/2005 2255 KRR EPA 8015B CCW CHANNEL DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 06/03/2005 0955 <0.5 mg/L 06/07/2005 2217 KRR EPA 8015B
Sequoyah Forebay Sampling
_Water Mean# of Water NOTES: %
Sample Date Mean # of % Sett Temr. Sample Date Wars asnati WaSUB Gavid Temp LOCATION SAMPLE COLLECTED BY SettlecAsrsicLOCATION TYPE
(,C) Clamnswn CC) Clam ____ ___
02/16/2005 0 0 7.9 02/16/2005 0 7.9 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 02/23/2005 0 0 8 02/23/2005 0 8 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 02/28/2005 0 0 7 02/28/2005 54 7 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/07/2005 0 0 7 03/07/2005 82 7 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/15/2005 0 0 10 03/15/2005 122 10 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/21/2005 0 0 10 03/21/2005 105 10 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 03/29/2005 0 0 12 03/29/2005 101 12 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe,JRigsby 04/05/2005 0 0 13 04/05/2005 525 13 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 04/07/2005 0 0 14 04/07/2005 252 14 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/04/2005 0 0 17 05/04/2005 0 17 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/09/2005 0 0 18 05/09/2005 632 18 60% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/12/2005 0 0 18 05/12/2005 549 18 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/17/2005 43 50 23 05/17/2005 106 23 0 Outside Plant Forebay Quant J Rigsby, M MacCarroll 05/19/2005 31 50 21 05/19/2005 299 21 33% Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 05/23/2005 579 50 20 05/23/2005 22 20 0% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 05/26/2005 242 50 20 05/26/2005 61 20 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 05/30/2005 1530 26 22 05/30/2005 1831 22 66% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/02/2005 1344 30 22 06/02/2005 1989 22 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/07/2005 240 40 23 06/07/2005 613 23 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/09/2005 192 40 24 06/09/2005 425 24 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll, Shaffer 06/13/2005 236 42 24 06/13/2005 928 24 0 Outside Plant Forebay Quant J Rigsby, M MacCarroll 06/15/2005 78 33 24.5 06/15/2005 2491 24.5 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 06/23/2005 0 0 24 06/23/2005 171 24 0 Outside Plant Forebay Quant W. Allen 06/24/2005 0 0 24 06/24/2005 784 24 0 Outside Plant Forebay Quant W. Allen 06/27/2005 0 0 24 06/27/2005 305 24 0 Outside Plant Forebay Quant W. Allen 06/30/2005 0 0 24 06/30/2005 0 24 0 Outside Plant Forebay Quant W. Allen
Sequoyah IIplant Sam pling SmlDaeMean # of %Stlr Water Mean# of Water SAMPLE Sample Date % Settlers Temp. Sample Date Asiatic Temp. LOCATION SUB LOCATION SAMPE COLLECTED BY 3
('C) Clams/m (,C) _ _ _ _ _ _ _ _ _ _
04/01/2005 0 0 14 04/01/2005 29 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/06/2005 0 0 13 04/06/2005 81 13 INPLANT Inplant Raw Water QUANT Dick Adcock 04/08/2005 0 0 14 04/08/2005 54 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/13/2005 0 0 16 04/13/2005 106 16 INPLANT Inplant Raw Water QUANT Dick Adcock 04/15/2005 0 0 16 04/15/2005 0 16 INPLANT Inplant Raw Water QUANT Dick Adcock 06/23/2005 432 50 25 06/23/2005 154 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/24/2005 162 80 25 06/24/2005 30 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/29/2005 290 80 26 06/29/2005 168 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/01/2005 318 100 27 07/01/2005 127 27 INPLANT Inplant Raw Water QUANT Dick Adcock
PERMITTEE NAMEIADDRESS (Include FacditVNarmeltocation if DifferentJ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000
____ffENTEOFIEBS-- - -- - - -- - TN0026450 101 G F - FINAL SODDY-DAISY TN 37384 IPT NMBER ISCHARGE NUMBR
_acility_ VA-SEQUOYAHNUCLEAR PLANT____
DIFFUSER DISCHARGE Location HAMILTON _ COUNTY_ __ _ _ _ I ONITORING M 'ERIOD L . EFFLUENT IYEAR I MO I D2AYIl YEAR I MO I DAY *... M^
- ftcrotLAMer r-- ...s ATTN: Stephanie A. Howard Froml 05 l 06 T 013I Tol 05 l 06 1 30 1 NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
__ ___ __ __ _ __ ___ __ _ __ ___ __ _ __ __ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE 0 1 62 0 30 /30 CALCTD CHANGE MEASUREMENT 82234 1 0 0 '-PERMflY< **** ""-' ********-,.,.., -
- t 2 } DEG C/HR - CONTIN' CALCTD' EFFLUENT GROSS VALUE f ,, ' > , ' UOUS' iEQIREMENT-.
-2 TEMP. DIFF. BETWEEN SAMP. & SAMPLE 04 2.3 0 30/30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 ; - ':sPERMIT ..* * -'"' '3'0-'* ' DEG. C. -CONTIN CALCTD EFFLUENT GROSS VALUE REURMN0 t ' .:L t'~Uo--
BORON, TOTAL SAMPLE <0.20 <0.20 <0.20 19 0 1/30 GRAB MEASUREMENT 01022 1 0 0 . ~t.->PERMIT.' ^w +****+* * ^.T .. -REPORT , . EPORT-r-MG/L ONCE/- 'GRAB.
EFFLUENT GROSS VALUE MOREQUIREMENTW SAMPLE MEASUREMENT R'EQUIREMENT --1I.
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under nmy direction or supervision In accordance wtilh asystem designed to assure that qualified personnel T J. Randy Douet property gather and evaluate the Information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information. the Information submitted Is. to the best of my knowledge and belief. true. rincipal Environmental Engineer Site Vice President accurate, and complete. I am aware that there are significant penalties for subnitting false Information, Including the possibility of fine and Imprisonment for knowing violations. SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a/l attachments here)
The following injections occurred: 1.PCL-222 Copolymer (max. calc. conc. was 0.019mgQL-iimit 0.2mg/) 2.PCL-222 Phosphate (max. calc conc. was O.O54mgQL-limit 0.2mg/)
3.Blodetergent 73551 (max. calc. cone. was 0.008mg/L-limit 2.0mg/L) 4.H-150M (max. calc. cone. was 0.037mg/Q-limit 0.050mg/L) 5.H-1 50M (methylene chloride extraction method was
<0.020mgIL-limit 0.050mg/I)
EP _RE For 3320-1 EPA Form 330. . (REV 3199) _e Prvu edtin .... ma be Pag 2 Previous editions may be used Page 2 of 2
PERMIrTTEE NAMEtADDRESS (Include Faci7ity Name/Locatlon ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMBNo.2040-0004 Address P.O. BOX 2000
___1NTEROFFIqE S13-?N- --- -- l TN0~026450 II 101 T l F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Lac y TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY EFFLUENT From0 *** NO DISCHARGE = "'
ATTN: Stephanie A. Howard 10_ 1 To 10 613 NOTF: Read Inasnidtions hbeore nomoelino Ihis form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
___ __ ________ _ ___ ____ ___ __ _ _ __ ___ __ _ _ __ _EX__ O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1/0 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 7;EMT'14.;****4..,.i*** 49* PERCENT QTRLY COMPOS EFFLUENT GROSS VALUE RQUIREMENT ; t-. . _;,._._ - ,, ,_. .
IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1/0 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 0 :.PERMITA., **** '. *' em 1..149 ; PERCENT - QTRLYI! COMPOS EFFLUENT GROSS VALUE ' .UREEN PV,_C0 MIMINU _ _ _ __
SAMPLE MEASUREMENT
- REQIJRE~vINT .. ....-
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1 SAMPLE MEASUREMENT MEAUIREMENT.'
SAMPLE MEASUREMENT
!'REQLUIREMEN10_ .
SAMPLE MEASUREMENT
- -PERMITh ' ...- .. 7-t3REQLIREMENT , .P . .'
SAMPLE MEASUREMENT NAMETrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and an attachments were prepared under my - i/ TELEPHONE DATE irectlion or supervIsIon in accordancewith a system designed to assure that qualified personnel k . !1L,,tJ4.Ad J. Randy Douet properly gather and evaluate the Information subrnited. Based on my Inquiry of the person or persons who manage the systern. or those persons directly responsible for gathering the Pincial Environmental
. . ~~~~~~~~~Informabion.
the Informstion subrnitted Is, to the best of my Vtovetedge and belief. truePicpiEvrnetlEgne 2 4-70 0 7 1 Site Vice President accurate, and complete. I am aware that there are significant penalties for subnittingfalse SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Information, Inctuding the possibility of fine and Imprisonment for knowing volatlons. OFFICER OR AUTHORIZED AGENT AREA NUMFER YEAR MO DAY TY EIRP I T D]________________________I_______________ CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled June 5-10, 2005. Report Is attached.
3/9 . ru edtin mab sdPae1o EP Fr EPA Form 3320-1 (RE 330-(REV 3f99) Previouseditionsmaybe used Page I of 1
July 11, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, JUNE, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected June 5-10, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values forboth species were
> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CTR 2L-M Attachment cc (Attachment):
Files, R&TA, CTR IB-M SQN June 2005M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: July 11. 2005
- 1. Facility / Discharger: Sequovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit#: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 3.266
- 7. lQ10: 2,992.4
- 8. OutfallTested: 101
- 9. Dates Sampled: June 5-10. 2005
- 10. Average Flow on Days Sampled (MGD): 1545. 1553. 1553
- 11. Pertinent Site Conditions for June 5-10, 2005 H-15OM was injected:
Injection Location I Date/Start Time (ET) Date/Ending Time (ET)
Essential Raw Cooling Water (ERCW) Train A 06/06/2005 1130 06/08/2005 1130 Towerbrom 960 was injected:
Injection Location Date/Start Time (ET) Date/Ending Time (ET)
Raw Cooling Water (RCW) 06/05/2005 0000 06/10/2005 2400 Essential Raw Cooling Water (ERCW) Train A 06/08/2005 1250 06/10/2005 1755 Essential Raw Cooling Water (ERCW) Train B 06/08/2005 1250 06/10/2005 1755
- 12. Test Dates: June 7-14. 2005
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101: 10.98. 22.0, 43.9. 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0.43.9. 72.0. 100.0 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: ICs,= 43.9%
- 17. Test Results: Outfall 101: Pimephalespromelas:IC75 > 100%
Ceriodaphniadubia: IC25 > 100%
UV treated Outfall 101: Pimephalespromelas:IC)5 > 100%
- 18. Facility
Contact:
Stephanie Howard Phone #: (423) 843-6713
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected June 5-10, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/ (MM/DDJYY)/ Arrival Initial (MM/DDJYY)/
Sample Time (ET) Time (ET)' Temp. TRC* Time (ET)
ID Collected Received (0 C) (mgJL) Used By 101 06/06/05 0522 06/06/05 1348 2.5, 2 .6 t <0.10 06/07/05 1414 06/05/05 06502t 06/07/05 1414 Intake 06/06/05 0550 06/06/05 1348 3.3 <0.10 06/07/05 1414 06/07/05 0628 t 06/08/05 13233 101 06/08/05 0528 06/08/05 1500 2.2, 2.8t <0.10 06/09/05 1323 Intake 06/07/05 0647 to 06/08/05 1500 3.1 <0.10 06/10/05 1456 06/09/05 0729 to 06/11/05 1342 101 06/10/05 0629 06/10/05 1402 2.9, 3.2t <0.10 06/12/05 1407 06/11/05 1320 Intake 06/09/05 0743 to 06/10/05 1402 3.3 <0.10 06/12/05 1407 06/10/05 0643061/532
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 A I.0 0 C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics.
Inc.) for 2 minutes.
3
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatic BioSystems, Inc. In-house Cultures
- 2. Age: 22.75-24.25 hours2.893519e-4 days <br />0.00694 hours <br />4.133598e-5 weeks <br />9.5125e-6 months <br /> old <24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 l
- 6. Test Initiation: (Date/Time)
Outfall 101 06/07/05-1414 ET 06/07/05-0941 ET UV Treated Outfall 101 06/07/05-1358 ET
- 7. Test Termination: (Date/Time)
Outfall 101 06/14/05-1321 ET 06/14/05-0850 ET UV Treated Outfall 101 06/14/05-1300 ET
- 8. Test Temperature: Outfall 101: Mean=24.8C Mean=25.0 0 C (24.3-25.3 0C) (24.4-25.30 C)
Test Temperature: UV-Treated Outfall 101: Mean=24.90 C (24.3-25.4 0 C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxvgen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted June 7-14. 2005 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 98 93 93 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 90 90 90 Test Solutions Mean Dry Weight (mg)
(% Effluent) 1 (replicate number)
______ 23 4 Mean Control 0.780 0.735 0.830 0.791 0.784 10.98% 0.925 0.771 0.775 0.738 0.802 22.0% 0.812 0.681 0.683 0.801 0.744 43.9% 0.858 0.843 0.728 0.797 0.807 72.0% 0.792 0W861 0.841 0.835 0.832 100.0% 0.818 0.770 0.829 0.907 0.831 Intake 0.835 0.730 0.706 0.526 0.699 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LCso: TUc = 100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted June 7-14, 2005 using effluent from Outfall 101.
Percent Surviving Test (time interval used- days)
Solutions 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(% Effluent)
(%flet I 3 4 Data5 (replicate 6 number) 7 8 ._ 9 10 Mean Control 29 35 32 27 32 31 31 32 32 32 31.3 10.98% 33 31 35 32 30 33 30 34 33 32 32.3 22.0% 33 35 33 31 28 33 30 33 36 34 32.6 43.9% 34 32 37 33 33 32 32 37 32 36 33.8 72.0% 34 33 34 35 32 32 32 36 35 37 34.0 100.0% 36 34 37 40 30 32 37 40 36 37 35.9 IC2 5 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9%
Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = I 00/LCs 0: TUc = 100/ IC2 5 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic! 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted June 7-14. 2005 using water from Intake Percent Surviving Test (time interval us d - days)
(%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(% Effluent) Data (replicate number) 2 l3 l4 l5 6 7 8 9 10 Mean Control 29 31 29 28 27 .30 29 30 30 30 29.3 Intake 33 30 29 321 32 32 31 29 30 33 31.1 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = I00/LCso: TUc = 100/ IC25 7
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted June 7-14, 2005 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100' 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 98 98 98 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(%Effuent) replicate nuer)
(w _ _ 1
______ 2 3 4 Mean Control 0.822 0.755 0.760 0.768 0.776 10.98% 0.940 0.812 0.788 0.710 0.813 22.0% 0.792 0.762 0.782 0.683 0.755 43.9% 0.882 0.899 0.850 0.765 0.849 72.0% 0.777 0.847 0.734 0.878 0.809 100.0% 0.767 0.866 0.767 0.722 0.781 Intake 0.760 0.843 0.902 0.851 0.839 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 5 0: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date l Time Duration I Toxicant Results (OC 25)
Pimephalespromelas June 7-14, 2005 1341 _ 7-days KC1 0.62 g/L Ceriodaphniadubia June 7-14, 2005 0950 7-days NaCl 1.05 g/L 8
PIIYSICALICHIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pintephales pronrelas and Ceriodaphnia dlubia Tests, Sequoyah Nuclear Plant Effuent (SQN), Outfall 101, June 7-14, 2005.
Test Sample ID Temper ture (QC Dissolved Oxygen (mgIL) ...jpi(i.I 5 ) Conductance Alkalinity Hardness Total Residual
________Initial Final Initial Final Initial Final (limhioslcm) (mgIL CaCO3 ) (mg/L.CaCO,) Chlorine (mgIL)
Control 24.9 24.6 7.8 7.6 7.75 7.63 301 61 83-
_____ 24.8- 25.1 24.3 - 25.0 7.6 - 8.0 7.3 - 7.9 7.65 - 7.85 7.42 - 7.76 294 - 308 58 63 80 - 86 10.98% 25.0 24.7 7.9 7.5 7.85 7.62 285---
24.7- 25.3 24.3- 25.1 7.6 - 8.1 7.3 - 7.8 7.73 - 7.97 7.43- 7.75 276- 290 - - --
25.0 24.6 7.9 7.5 7.84 7.63 274 -
z22.0% 24.7 - 25.2 24.3 - 25.1 7.6 - 8.2 7.3 - 7.8 7.72 - 7.96 7.45 - 7.78 268 - 280 - - --
4 49% 25.0 24.7 8.0 7.5 7.82 7.64 251 --
tI 2. -__25.2 24.4 - 24.9 7.6 - 8.2 7.3 - 7.9 7.71 -7.95 7.49 -7.83 245 - 258 - - -
2.% 24.9 24.6 8.0 7.6 7.80 7.66 222 --
_____ 24.7 - 25.2 24.3 - 25.1 7.6 8.4 7.3 - 8.3 7.70 - .7.92 7.50 - 7.85 211 - 228 - - - --
100.0% 25.0 24.7 8.0 7.7 7.77 7.68 193 72 80 <0.10
_____ 24.7 - 25.3 24.4 - 25.0 7.6- 8.2 7.3 - 8.3 7.65 - 7.91 7.57 - 7.83 183 - 202 71 - 74 80 80 <0.10 - <0.10 Intake 25.0 24.6 8.1 7.7 7.86 7.70 192 74 81 < 0.10
_____ 24.8 - 25.3 24.4 - 24.9 7.6 - 8.5 7.3 - 8.3 7.70 - 7.95 7.57 - 7.89 180 - 201 73 - 75 78 - 88 <0.10 - <0.10 Control 24.7 25.1 7.8 7.9 7.75 7.75 301 61 83 -
24.4 - 24.9 24.9 25.3 7.6 - 8.0 7.8 - 8.1 7.65 - 7.85 7.60 - 7.89 294 - 308 58 - 63 80 - 86 10.98% 24.7 25.1I 7.9 8.0 7.85 7.75 285 --
24.5 - 24.9 24.8 - 25.3 7.6 - 8.1 7.7 - 8.2 7.73 - 7.97 7.61 -7.89 276 -290 - - --
20% 24.7 25.1 7.9 8.0 7.84 7.74 274 - -
20% 24.6 - 24.9 24.8 - 25.2 7.6- 8.2 7.8- 8.2 7.72 - 7.96 7.61 - 7.89 268 - 280 - - - --
24.8 25.1 8.0 8.1 7.82 7.75 251 ---
_____ 24.7- 25.1 24.9 - 25.2 7.6- 8.2 7.9 - 8.2 7.71 - 71.975 7.63 - 7.89 245 - 258 - - - -
72.0% 24.9 25.1 8.0 8.0 7.80 7.76 222--
24.8 - 25.1 24.9 - 25.3 7.6 - 8.4 7.9 - 8.2 7.70- 7.92 7.64 - 7.90 211- 228 - - -
10.% 25.0 25.1 8.0 . 8.0 7.77 7.77 193 7280 < 0.10 10.% 24.8 - 25.2 24.9 - 25.3 7.6 - 8.2 7.9 *- 8.2 7.65 7.91 7.66- 7.91 183 - 202 71 - 4 80 - 80 <0.10 - <0.10 Intake 24.9 25.1 8.1 8.0 7.86 7.77 192 . 481 <0.10
______ _____ 24.7 - 25.2 24.8- 25.2 7.6 - 8.5 7.9 - 8.3 7.70 - 7.95 7.65 - 7.92 180 - 201 73 - 75 78 - 88 <0.10 - <0.10 Overall temperature ("C) Average Minimum Maximum Pimephales promelas 24.8 24.3 25.3 Ceriodaphniadubia 25.0 24.4 25.3 9
PIIYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimneplhalespromelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, June 7-14, 2005.
Test Sample ID Temper ture C0 C) Dissolved O ygen (mg/L) pH (S.U.) Conductance Initial Final. Initial Final Initial Final (pmhos/cm)
Control 24.9 24.7 7.9 7.4 7.80 7.62 294 24.7 - 25.2 24.3 - 25.1 7.7 - 8.2 7.1 - 7.9 7.68 - 7.90 7.50 - 7.74 286 - 309 10.98% 25.0 24.7 8.0 7.4 7.81 7.62 287 24.8 - 25.2 24.3 - 24.9 7.6 - 8.2 7.1 - 7.9 7.71 - 7.91 7.50 - 7.71 282 - 293 25.1 24.7 8.0 7.4 7.80 7.63 276 22.0% 24.8 - 25.3 24.3 - 25.1 7.6 - 8.3 7.2 - 7.8 7.71 -7.91 7.50 - 7.78 272 2 282
> 39/ 25.1 24.7 8.0 7.5 7.80 7.63 25 256 43.9 24.8 - 25.4 24.3 - 25.1 7.6 - 8.3 7.3 - 7.8 7.71 - 7.91 7.50 - 7.77 249 - 263 72.0% 25.1 24.7 8.0 7.4 7.79 7.63 225 24.9 - 25.3 24.4 - 25.1 7.7 - 8.4 7.1 - 7.8 7.71 - 7.91 7.50 - 7.75 221 - 232 100.0% 25.1 24.8 8.0 7.5 7.77 7.67 196 24.9 - 25.2 24.4 - 25.3 7.7 - 8.3 7.1 - 7.8 7.68 - 7.91 7.50 - 7.78 190 - 208 Intake 25.1 24.8 8.2 7.5 7.82 7.65 195 24.8 - 25.4 24.4 - 25.1 7.8 - 8.4 7.3 - 7.8 7.73 - 7.93 7.51 - 7.83 189 - 211 Overall temperature CC) Average Minimum Maximum Pimephalespromelas 24.9 24.3 25.4 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected June 5-10, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. PimephalesPromelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured by EPA Method 170.1.
- 5. The pH was measured by EPA Method 150.1.
- 6. Conductance was measured by EPA Method 120.1.
- 7. Alkalinity was measured by EPA Method 310.1.
- 8. Total Hardness was measured by EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KC1 or NaCl.
Sodium Chloride (NaCi crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimatin2 the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
- 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).
14
Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine,
- Diffuser Discharge Concentrations of Chemicals Used to Control Growth
- of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,.
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 09/08/1998 9!$:0.015 _
09/09/1998 'X02o0 >
09/10/1998 0144>2tS2^r; 09/11/1998 ,'0. 13,k 09/12/1998 . '0O _i 09/13/1998 XtO.O°1 09/14/1998 r6d'2 0 02/22/1999 L20.O0124 02/23/1999 -.0'0-' q 02/24/1999 Aooolr9gV 02/25/1999 02/26/1999 - -0 02/27/1999 ' OOOA 2f 02/28/1999 o:oort-2
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -June 10, 2005 3
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 10/06/2002 0 10/07/2002 a 10/08/2002 10/09/2002 ql' 10/10/2002 N 10/11/2002 4
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - June 10, 2005 5
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12i 1998- June 10, 2005 6
Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1__ of 3_
Clicnt: TVA Environmental Testing Solution, Inc. Dclivcred By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FcdEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): is >)
Facility Sampled: Sequoyah NP 28801 General Comments:
C,7b-c l SeALS OTOCl SAVPL NPDES Nuber: TN0026450 Phone: 828-350-9364 &e i,)
. 6ccb CaoNIT7NoI.
Collected By: Wanda Allen Fax: 828-350-9368 Field Identification / Grab/Comp. Collection Date/Time Number FlOW M.RnEe?.
Sample Description Number& MGD Rain Event? V.....
Volume (Mark as Appropriate) v
-H Date
____________InchesNune Ti C.......ected CollecteCd Yes IfYe, No Trace: ;.TS Log AnaTime CJsc tApper SQN-101-TOX Comp 6105/05 - 6106/05 0 2 (2.5gal) NA V = . ] '>
SQN.INT-TOX Comp 6/05105 - 6106105 Of7/ 1 (2.5 gal) NA .- .J... 5.r. j -
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Wanda Allen 06/06/2005 Express Courier 06/06/2005 A1aJc Ld-t&j 4 09415 IF. ~~- M Express Courier . 06/06/2005 j ETS / 06/06/2005 L
+ . + t Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. 13iomonitoring samples are preserved by storing them at 6C and shipping them in ice. Trhe hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time or collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivcry or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 2_ of 3_
Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Soquoyah NP 28801 General Comments:
NPDES Number: TN0026450 Phone: 828-350-9364 t._ ooc(
LAAc. b Collected By: Wanda Allen Fax'- 828-350-9368 Field Identification/ CrOab/Comp Collection DatTime Contniner Flow ." ? ? -.
Sample Description Number & (MGD) kn Rai Appropite Volume (Mark as Appropriate) sq tbltrY(~t.
__ _ ~~~~~~~Collected
_ _ !..i. .- i;i - 'es t 'j i ?;-
Date Time lftYed, yCs l No l a
-my Tep: ." Tim ipABy -
SQN-1 01-TOX Camp 6/07/05 - 6/08/05 00 2 (2.5gal) NA - - - . f SQN-INT-TOX Comp 6/07/05 - 6/08/05 / 1 (2.5 gal) 17 NA _ V ^ . it'.to'.
Sample Custody- Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Wanda Allen 06/08/2005 Express Co 06/08/2005 Express Courier 06/08/2005 3, f71A ,,B 06/08/2005 IsCO Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. IDiomonitoring samples are prcserired by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time fi-amc.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
_/_ _ _ _
BIOMONITORING CIiN OF CUSTODY RECORD Page _3_ of _3_
Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Project Name: Scquoyah NP Toxicity 351 Depot Street FedEx UPS Bus Client P.O. Number: N/A Ashville, NC Other (specify): Express Courier Facility Sampled: Sequoyab NP 28801 General Comments:
NPDES Number: TN0026450 Phone: 828-350-9364 CIl 0 0'L -A.0&
C S a01J 0 ;oot C0,010 Collected By: Wanda Allen Fax: 828-350-9368 dl Field Identification/
Sample Description Grab/Comp Collection Date/rime Container Number &
Volume Flow (MGD) Rain Event?
(Mark as Appropriate) 5, L
B
________ _ _ Collected - - --
Time Y sDateIf YesN No Trace S Arvt.< Temp By Tim Appear-:
SQN-101-TOX Comp 6/09/05 - 6/10/05 672 2 (2.5gal) NA t j _ ti .iZC- w X SQN-INT-TOX Comp 6/09/05 - 6/10/05 t1 1(2.5 gal) NA =0CSl Z Sample Custody- Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Wanda Allen 06/10/2005 Exprcss Couri 06/10/2005 Express Courier 06/10/2005 06/10/2005 t'0.
. ~02
/1"17.
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samplcs are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FcdEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
rnvironmental Testina Solutions. Inc. Page I of 6 1nvironmefltal Testin2 Solutions. Inc. Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA County: Hamilton Facility: Seauovah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: -T-ziV't-Dilution prepar tion info mation: Comments:
Dilution prep (%) 10.98 22 43.9 72 100 Effluent volume (mL) 274.5 S50 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:
Organism age: 22 .-IS -ro 2- .-ZS q eU Randomizing template: ,lfm a Date and times organisms tOa-QIc 4S t330 %S00 Incubator number:
were born between:
Organism source: & frC..ik O6b0-1 OS Artemia lot number !
Transfer bowl information: pH = Temperature = °C Total drying time:
._ _. S3 . 2'4.8at Date/Timein:C& 1'15 13L4S Average transfer volume: q.
Li D Date / Time out:06-IS i
. . Oven temperature: k'c Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time Igs 0 Cb6-zS ____llA 06-UAcisO i 07-oO 1 io<
clSSZQ Icco * <)Ao-oS J eovco2.4s -
2 not-os0 0c95s I Isss 1-i c3w3o-04 5 C. OScrOO.&z h z 3___ -4Cso I . - C.-VA Cr vSeaA-.723 s 6-12-o¶5 Q RS. ISoo _ Ol1 34-OS G QSob. 16.L-AGC 6 06 1-G5d' Oiso 1S6.! 521! 06Q01t0516 ioc*4 r 6 640 Control information: Acceptance criteria Summary of test endpoints:
%Mortality: s 20% 7-day LCq0 _____ __7_
Average weight per initial larvae: I .18LI * -*;k NOEC 10b%
Average weight per surviving larvae: O.181 2' 0.25 mglarvae LOEC > lot,07 ChV' 15 lC£4 CIS > Ic o;
.,Environmental Testing Solutions, Inc. Page 2 of 6 r:.'.:
Species: Pintephalespromela. Date: tol-o-1-OS lTi
-. Cltent:* TVA ISequoyah N'uclea ar Plant - Non-treated Survival and Growth Data Day CONTROL 10.98% 22%
A B C D E F I G H I J -1 L
_ __ IC). it) 10 to 10 10 I 10 10 I_
. llo C /0 10 /o /0 /0 10l t 10 /0 14 1- 10 10 10( 10 / 1010
/6 0010 3 _ji .*_ IC MO 10 1O I~ I_ IC) io /0b0t/ to to _/0 to/0 /0G0/0 _I'0 5 - it- _ _ -
_ /_ o__/0 lo /0/0 10 / 10_I
_ _0/0 6 10 t0 IC) IC) 1o0 10 0 /0 '~ /R /0 I 7 to I- Iv-i 2jjr -
ZV5d
_ / I___ 0j /0/10/10/0 I o 1 A -Pan weight (mg) e _
Color Ident'i on: _OAp! lqso SO,1 a .q4 l1 1"v ,l 4 q1 Ii91i.3? z 1 K .oq ,15.t lq.1 IS .o 1t4.5B Analyst:
B- Pan + Larvae welght (m~e)1rt t.30 ZZ U.7e Z1,22-1-2. ZS3.6 T02 2z; q 2 1,-L4 41 Z3 .21 lCi21.01tbt 2Z-Sq Larvse weight (mg) - A - B 1.30 1.?S 8.o0 1A it.s In k 1.15 1.3k 8.12. b t .A .
Weight per Initial number of larvae (mg)
- C / Initial number Of larvae Average Percent
%0' 0* f3Ž'~ Ifo~
weight per reduction 0 Initial from control o,18q 0. ,0 - -. ,I,. -6. I -7.
number of (%)
Comrfment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w - wounded.
Calculations and data rcviewed:-
Comments:
'I.
Page 3 of 6
- EnvIronmental Testing Solutions, Inc.
Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: ie-,L-M~-CrS Survival and Growth Data Day 43.9% 72% 100%
__ l N 0 O P Q IR S T U V W X 1 00 ICI__ 1/0 1) 10 /0O 1C) 10/ O 10/0
. z(01 010 0/01C D oS} 0 '0/)I
/010C) /0
. .}
/0/ 10 10 /01/01 0to 6 / /O /0 3_ - 0 /_ _Oi /0 /O /o /lb 1 _ _10
-. ' 3 to lo 1/()t 1/(lO o IC) Io: Io lo IC) /O "i~t 5 5 .10 /0 lO 1O /O t 10 O 10 /0 10 0O i,4 tI--, 6 7 lo 1L 1O /)
/
/L /O
/0/0/0
/L /
/O IC)
/C
/ Afl I(
I.s .. A -Pan weight (mg) &AU(
Color identlficatlon 6eA144 t; Id tS 1Nq1 S."A jq,98 Isml l, I.11 1 1'.. V53 q t15.Io 1L4.31 I.&-'..- Analyst: AA.4 B-Pan+Larvae weight
- ~
.I:-- . Analyst: -t - -Z I-l Larvae weight (mg) - A - B O.58 1.^1a7.z8 -1.9 42. R.('1 E.&AI j.35 -.12 1.10) 8.sl 9.01 I.,t. Weight per initial number
- 3. of larvae (mg)
-Clinitiai nunber orlarvae b0 10* )* 9.°
.. ... Average weight per Initial Percent reduction from control 0.80-71 2,91o 0. 3 -bO2l number of (%)
larvae (mg)
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed: _
Comments:
4 Page 4 of 6 Envi ronmental Testing Solutions, Inc.
Species: Pimephalespromelas .
Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: of.-o1-Os Survival and Growth Data Day 100% Intake
3 lo0l 1,0 1 10 0 10 900 1I C)id IC
_ __ _ 4_
IC) lO 10 J S__lO_ 10 10 'd 1 0 A - Pan welght (mg) 6LACK Color Identification:_ F_._14 s.I Analyst: t 4 4 .I 4 ,q q B - Pan t Larvae weight Analyst: , 21dc Z.ZtS 21.-1 tZO.22.
Larvae weight (mg) - A - B 935 1.30 1.0b <.52G Weight per Initial number of larvae (mg) 4 AP t°'
U ClInitilanumber of larvae Ob '
I.. Average weight per Initial number of larvae (mg)
Percent reduction from control
(%
q 6(Dq'1 I Comment codes: c clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
TVA / Sequoyali Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Chronic Whole Effluent TosicltyTest (EPA-821-R-02-013, 1I'ethod 1000.0)
Species: Pimephatlexpromelaz Quality Control Verification of Data Entry, Calculations, and Statistical Analyses calet: TVA / Sequoyal Nutlear Platt, Outft3l 101 Not-trted Tesit datts Jut. 0114, 2005 Project au-btfr it" Revelwed N4otforeCopliancoAaesgs atrssn LI--./ atra t.tr Ite t I I
P.h.ew.kiI bi IV..inrh.. ee,tae..I F.. e..i .. e.e 0.
t.
C-_*_tb AL. T t7s}
_ t.
- or Ib Mh.. f k(h a . IN*hdU(,4i") r*r k ts e .- kt.(b.,,.. Or IW *ee0.Dl 1%)
C'- I0 0.7s0 Coatrol 0.714 5.0 0.715 I000 0.7t4 5.0 Not applicable 0.830 0.791 _ _ _ _ _ _
0.925 0.302 10.4 0.771 100.0 0.802 10.4 -23 IO.98% 0.775 0.73s 30 22%
10 10 0.309 11.6 i0.6ii 9L. 0.744 9.7 11l 10 0°t13 31l 10 N 10 0.t07 7.2 0.4 72t tO.O O.tO7 7.2 -2.9 30 0.721 0
-r 10 0.792 10 R 10 0.e32 3.5 *00.O O.61 0.832 3.IS -2 72% 8 0841 0.t41 T 14.71 23.06 8.35 0.-35 tO 14.79 0.813 V 30 14.53 7.70 0.831 6.8 0.770 300.0 0.833 6. -6.0 100% 0.329 10 15.10 x _30 14.39 0.907 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
. Y 10 ;0 14.74 0.35 0.335 10 10 35.15 2245 7.30 0.730 0.730 100% latskt z 0.7175 5.9 90.0 0.69 18.4 I0.t 10 9 14.65 21.71 106 0.714 0.706 nn 14.96 20.22 S 26 0.751 ,__, 0.526 I I__. ---- I .to . ____ 7
_ _ . , A outran 101: MSD - Minimum Siificant DifTcrence Dallett's btISD valut: 0.1012 PMSD - Percent Mimrim Sipifct Dierence PMSD is a nowuure ofte preeision. The PMSD Isthe ninismim Percent diffnce between the control and troatznt that can be declred etwatistaly ofiflnt Olaeluent toxiityteay.
eiob FbSSD: IL9 Oa averenf a sipificast difference oceure for Envirocnrtal TeatingSolution Inc e chronic toxicity tells wtent a toxicant reduces lmephola es by 14.0% intih ot (detir ed Intake: tlhmun reference toxican te tinia D eltt's MdSDvalue? 0. 1304 Lower PMSD bound detnined by USEPA (1Othlpercnte) - 9.4%
PMSD: 16.6 Upper PMSAD tbund detemine by USEPA (90th peentil.) - 35*%
Th' lower and upper boonds were clsculated by the USPA usIng 205 tesas conducted from 19 lsbormtores for Flmephater growth in chronic reference toxalant teat)
USEPA. 2000.t ndenatndintand AcocuntingforMMtod Variabiity in Whol Effluent Toxicity Appliations Utder theNations Polutant DishargeEliniation Pror BEPA-833-R-00003. Us Envionmtl Ptedion ACney. Ct-clnat(OIL
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Statistical Analyses LarI Fith Crw1 nsd SanivullTut.7 Day Grwth StartDotw 617 TastID: PpPP SamplID: WAiSequoyahNuckrrPbl l0l -Non-treated Rod DstL: 6d141M Lab IID R3-iE Taibg Sol SapDV Tp'. DMR-Dkbar MoDtatme F.pozt Sampe Dat: Prhosowt FWJCHR.EPA421.R.02-03 Tat Spees: PP*Pkonphai prombs r .e.% 1 2 3 £ D-Contol 0.7r00 0.7350 0.e300 0.7910 10.91 0.92S0 0.7710 07750 0.7310 22 0.1120 0.6tlO O6t30 0.1310 43.9 0.2580 0.U430 O7280 0.7970 72 0.7920 0.1610 OU410 0.e350 100 0.2180 0.7700 0.1290 0.9070 Tn"sMm: Uatrbatfnmd I-Tallk Cae.% M.1. N-Mam M Mem Mia Mal CV% Y. I-Stat Crhdal MSD M0. N-Mm D-Control 0.7U40 1.0000 740 0.7350 0.1300 4.985 4 .OOO LOXO 1032 0.832 1.0233 0.8023 0.7380 0250 10.401 4 -0.433 2.410 0.1012 0.8000 1.000 22 0.7u43 0.9491 0.7443 0.61n0 0.1120 9.67 4 0.946 2410 01012 0.8000 ILOO 43.9 0.8065 1.0217 0S065 0.7220 0.2120 7243 4 .0.536 2410 0.2012 02M 1.00O0 72 0.8323 1.0615 0.8323 0.7920 02410 3.490 4 -1.149 2410 0.1012 0.8000 1.00:0 200 0.2310 1.05" 0.1310 0.7W0 0.9070 6U32 4 *1.119 2410 0.1012 0.000 1.0000 Amiz"arTets Statlis Critic Ske Kert SlaPro-wtsTathtdkatosnoruldsntutxen(p>o.oi) 0.961619651 .18.4 0.45134646 .0.36290127 B EtrsTntkidictes qualvaram(p-0.62) 3517258167 IS.01627224 H"pOdhItTest(14asL OS) NOEC LOEC EV TU MSD MSDp MSB SKE F-Prob dt DtmosS Talt 00 >100 I 0.1012146240.121C0215 0.004329942QCt03527525 0.3363242SI Iti Treaensnb woD-Conkol Ll.nmr laterpolatim (200Rnm srpia) i.t. % SD 9S%CL21rp) Ske.
ICOS >100 ICIO >100 ICI >100 1.0 IC20 >100 DI 1C25 >300 2040 >100 0.8 IC50 >100 0.7 0.6 3 0) 0.2 0.1 0.0 .. .....
- 02
-0.2 0 20 40 so 8o 100 120 Dogs.1 Do.-Ruspams.e Pt1 1
0..
0.8 0.7 1-ta11.0.05 level
~~
0.6 ~ ~
............... ~ ~ o osigriicaee 0t 0.2 O.1 sqe06745dala
TVA / Sequoyah Nuclear Plant, Intake Non-treated June 07-14, 2005 Statistical Analyses LaetJ Gswtk 4SarvtnT mt-7Dyvwtb Sturt Dol 6el2GM= Test IM. PpPRCR Scnr'cm TMVA/ ShmbihNckax Phlnt Intake - Non-tvated Ead Dtme 6tl4/2005 Lab mr WZs vk. Tuft Sot S ,ic 1pke: DM,-Dbcbarr M-flatig Repuat Samph Dte Pwoocot PWCHR-EA4t21-R-2413 Test Specis: P1'Fksrpiales ptnekbs Come-% I 2 3 4 D-Coaft 0.7100 0.7350 300 0W7910 100 0.1350 0.7300 0.7060 0.5260 Trasserse d 1T4Il IUfsasr lsotosk CaC-% Mena N-lmm Mesa ?.Ml Met CV% N etSt t 3rkd M.ISD Mean N-Mns D-Conktot 0.7340 I.OXO 0.7340 0.7350 0.1300 4305 4 0.7n40 I.OX t00 OM3 0.919 0.6993 03260 0.1350 hI351 4 1263 1.943 0.1304 0.6"3 0.8919 AsuzkiryTab Statistc 05tkncs Skew Kart shpfno-W&s Testkbdicatesitnmaildertzfutim(p>001) 0.9Z1743MS 0.749 075565406 2306717611 F-Tat kAkstes equial vkin (V - 0.0) 10.7190221 47.46722794 lyMins Tmt(1-bJ4 0.) AISDe MSDp M.Sa MSI ".Prob dr Homoscedutsw I Teat kidwta no sipnifcant dilffmces 0.130372714 0166291727 0.014365125 0.009002792 0.25331911 1.6 teaftmcb VsD l-Conrol Uear nlterpolation (200 Remsmples)
Point % SD 95%CL!rp) Skew ICOS- 46.254 C10' 92.507-IC15 >100 1.0 IC20 >100 0.9 IC25 >I00 1C40 >100 0.8 IC50 >I00 0.7 mdMt3 IC ent ate less Uran te bkiest cmcutstm
- 0.6 o.s
~0.4 0.2 0.1
.0.0 0 20 40 s0 80 100 120 Dose %
D.s..Iapeo FPbt 9.11 0.8 0.7 I1-(aO.0.5 levI I.............................................................
of s~gnifcarce
= 0.6 0.5 a" 0.4; I 0.3.
02 0.1 I'a xqs_C"6-07-05dof*
Environmental Testing Solutions, Inc. Page 1 of 7 Environmental Testing Solutions. Inc. Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia Client: TVA County: Hamilton Facility' Sequovab Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: '8°A Dilution p a n information:l Comments:
Dutionprep(%) 10.98 22 43.9 j 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:
Organism age: 4 z4-Aoun Wt' Randomizing template: A .
Date and times organisms O-06-o-o. % -TS VZ... Incubator number and_
were bom between: shelf location: ____\
Organism source: OS-N-OS A-A YCT batch: OS145lcs Transfer bowl information: pH = 1. t1 Temperature 3 z Selenastrum batch:
Daily renewalinformation:
Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used
_ termination time __ __
O Coorcn-a Ogg I 06-eq-I OS00601 07 -C>w 4-to 4 2GM-CR Ss oib-Q* - Os9u 65 R1O~
1Q. . 0o~-c-Ss f
-A-l A6 c~o C. aow.b io. zz
- z3 z AAt 3__ ^t>~-cbS n5 L. QX->CS G tt ~ob.Zd Z3:
5 6_c____S O90o' 06-L 6 5olmg GSC C i Z'3 :
6 __l_-__ o09'1 *Ob-o)015ot&. $_ .V.3 At Control information:
6 I IR__ 2 Acceptance eziteri Summary of test endpoints:
_ _ _ _ __0 _ _ _
% of Male Adults: .**. O2. s 20% 7-day LC50 7' I7o
% Adults having 3 Broods: io1r, 1C6'.f 80% NOEC l CX)'t Mean OfEspring/Fcmale: SY3 29 -3 15.0 offspring/(emale ChV 7 lOOT.
%CV: s. R7.
<40.0% IC25 > IG0fo .
Environmental Testing Solutions. Inc. Page 2 of 7 Species: Ceriodaphniadubia Client: Seguova hNuclear Plant - Non-treated Date: O- - 05 CONTROL- 1 Survival andReproduction Data Replicate number Da 1 2 3 4 5 6 7 18 9 10 I Young produced n Q O OQj 0 O lO o Adult mortality %--_ L- - L- X- l t-2 Young produced Cb t C0 E C l( )l Adult mortality %.- _ l t L . _
3 Young produced _i n I 1 .c o_ C) _ i Adult mortalihy L-' _ L l L- -
4 Young produced 5
_ _ I 5 _
Adultmortality _ l l C l s Young produced 1LQ.+/-z 0 +/-LIL 110 jL O ii .j..
Adult mortality l-- L _ l L l- t- \.-
6 Young produced Adult mortality l
lQ o O-4 L O a l_
a 7 Young produced l a ll Lk I I 1=1 l5 1a Totalyoungproduced. l Z9 I 3Z Zi 3Z 1 31 3Z l Z 3 Final Adult Mortality 1 t- L X for3' Broods I X l I % I% *C I
Note: Adult mortility (L - live, D - dead)
Conceitration:
l%Mortality I C7 Mean OffspringIFemale: 1.
CONC: 10.98% Survival and Reproduction Data j
ii_ Replicte number D_ _ 3 l 6 l 7 9 10 il Young produced Young produced Adult mortality L I
C_)2<L Og l 4kc 3L 9 .L-. 1 3
Young produced l a - -
C -
0 C) I a-I a
I aQ-=
Adult mortality l L l 4 Young produced l Ll 5 L SI s Is I I Is 4 . . .
J Adult mortalIty L...1 al L a I t_
S Young produced l l\ l l \ l 1_° =
Adult mortality lI. 1 I 1 6 Young produced l OO 1..0 4 Adult mortality l L _ L ll L l 1 7 I Youngproduced 15 l 1 I1s IM 1 kD l LO 11 j 1-I Total young produced 3 313 351 a3L.3o .33 l 3o 3 l 5L 3 l Final Adult Mortality t*Cl l Il - l - l Note: Adult mortality (L - live, D - dead)
I Concentration:
I
%Mortality:
Mean OffspringFemale: 7 32-3 0 I
% Reduction from Control: -3.2Z7.
Environmental Testinz Solutions. Inc. Page 3 of 7 Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: Q!6-01-QS CONC: 22% Survival andReproduction Data
. Replicate number Day I l 2 3 4 5 6 7 8 9 10 Q 0 G O O O Adult mortality . U U U . U U U.
2
-V Young produced I
l C)_ Q Q Q QO l U U I 1-I I -
I- -
3 aIAdult l Young mortalityU produced looI U.
Adult mortality .c 4 Young produced S 5" 1 57 l Adult mortality I U 1 U j J U U Il 1I'l t l -
r, C-)z S
67 Young produced Adult mortality i E L-l T- ..
1 -
~. .Q.. L~. lonpoue 5
UIU i i L
ol ii o I o J - ll I o rL lZL4i 7t I . Tol.~>o lAdultmortslLty JU1 U l. U jL _. 1 _ U_ U. U.
Young produced I IS l I (.l l I l 1LI -Io I Total young produced l 3 3l 3Z5T l 33 T I aL Final Adult Mortality . 7_lll U
- U U Note: Adult mortality (L = live, D - dead) l Concentration:
% MortalitY: j° I Mean Offspring/Female: l 32.( l
% Reduction from Control: - 4.V7.
Co.NC: 43.9% Survival and ReproductionData Replicate number Day l I 2 3 4 5 1 6 l 7 8 9 10 i Young produced l I** 0 .Q Adult mortality U U. U I [ U U I U. U.
2 l A Young produced () o l .
Adult mortality l Ull t .
3 Young produced 0 C):r--- (1 -e i -I l lAdult mortality f _ll C L- I 1C7 4 Young produced [5 s(FI is 1 7s I l 6 i Adult mortality [ U l l- lt__ %, k**
. U U. UJ S lYoung p rodued -t l I I 3 1 Adult mortality C U U. U.
6 Young produced l Ql I___Adult morta t _LI Total young produced 2:3. 1 T3I3 .33 I3Z. j3Z 3 3-1 t3 3a l Final AduIt Mortality Note: Adult mortality (L - live. D - dad) l Concentration:
%Mortality: 07,0 Mean Offsprine/Female: I 33. 8
% Reduction from Control: -8. 07.
Environmental Testing Solutions. Inc. Page 4 of 7 Species: Ceriodaphniadubia Client: Se uovah Nuclear Plant - Non-treated Date: cL-,-moS co-4c: 72%
Day Adl K 1 2 3 Survival and Reproduction Data 4
Replicate number=
rS 6 7 S 9 10 Young produced 7 Q C) lAdult mortality lL@L Ilt @Ll t l- L-2 YoungAutmortality produced TI Adult mortality _ *T 3 _Young produced Qc -Q a 0 Adultmortality L L-* L L-- L >
s Young produced -L I L ST _
Adult mortality l l l. L S Young produced l1 l 31l C) C. 1 0 '
Adult mortality L-.~ L _1~.- . J_ _ L..I t'-*l 1_
Young produced 8 1O C- - O(i li Ili 1 ick Total young produced l 3'- lz b za 32. z tu as 1 35 Final Adult Mortality c
- 1 l 1 l T ll L Note: Adult mortality (L - live, D - dead)
. . Concentration:
%Morialty:a Mean Offspring/Female: l 34. C)
% Reduction from Control: l - V7.
CONC: 100% Survival and Reproduction Data Replicate number Day 1 2 T3 4 l S 6 7 9 10 j Youngproduced C) O °'O T I °O l Adultmortality _ pt l - l l-l l2 lYoung produced lCB %l t1 5)IOIO C)O C)~
Adult mortality l \- \l - I.-
3 Young produced (O lOL. C)
Adult mortality Cl T l L l ._ L l LL L_
. 1Youngproduced l b lAdultmortality 1 L 1 +/-IJ Youngproduced j 3 j10 ii i 3 Adul mortality L1 ll- . . L L l t l i6l Young produced l & lO o l8lO nt C) C) C) l1 Adult mortality l .l tl lIi 0 18 ZO I )1i ° C 7 Young produced Total youngproduced Final AdultNloaltv I I3CT 3q
. I I
.5 1 T4l T I I 30 j(
- lW 3Z i
31 l cy 3b l1 IoItliI Note: Adult mortality (L - live, - dead)
Concentration:
I Mortality: o C r.
lMean Offspring/Female: 35.9 l% Reduction from Control: l-1t.-177
Environmental Testing Solutions. Inc. Page 5 of 7 Species: Ceriodaphniadubia Client: Seguovah Nuclear Plant - Non-treated Date: c..-Oi-cnS CONTROL -2 Survival andReproduction Data Replict- number D A r 1 2 3 4 5 6 7 8 9 10 I Youngproduced t) l cO C a l v Adult mortality L L- L l -
o~o l
2 Young produced QC)2 C O l O o l0. o
_ j Adult mortality I I \J 'I 3 Young produced edr c Cl Adult mortality l 4 Young produced -- 5 l L A3 Adult mortality Y p J _. U. \_- l Lt L -...
5 Youngproduced l l I O. I I i Iw . I 0o( 1 Adult mortality l-L I 1 U L l L- L-6 Young produced jC) I j* O O 0 j O 10 l Adult mortality l U. l l L \. - - l ll -- - I I'S I I cA II I 11 %L>o I ICl~--
7 Young produced Total youngproduced . ' 29 2 Z-1 -3 1 Z9 3- a =5
.'O Final Adult NM9rtality Note: Adult mortality (L
- live, D - dead)
Concentration:
%Mortality: I 0C7 Mean Offspring/Female: lAz .
_ Inx--
- LUNU:
tlUU rew nno//0 RUL4RX i"I V" IV&va
-- j AV JreVa"LL&&lv1aaU n -P Repliet. number Day 1 9 j 10 oO C a) l 2 3 4 5 6 7 l I 1lYoung produced Adult mortality l C) lR lnlO C)1t 2 Young produced )
[3 Youngproduced C). C) C )
Adult mortality IL ! ! U- L L-4 Young produced 5lI L I L l Adult mortal!tr l r l j JU L-l .I7 4 Young produced 5 I l C I C) C)
Adult mortality I L- L- l_ *. T l k \.-
Adult mortality L- - -, L--- L Totalyoungproducduced~ VI lO Z.j0 O 1 29 tO 05 7 Yung produced IS I a I 6 I I , 1. . . _
l nalAdult Mortality 1C TlL- l Note: Adult mortality (L -live, D - dead)
Concentration:
-* Co wo&,* f'AAz
%Mortality: I 6-7 ot GC54 j I Mean Offspring/Female: 131.1 I % Reduction from Control. - 6.17. 1
TVA / Scquoyalh Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Verification of Cerlodaphnla Reproduction Totals Control-1 72%
ID I R. U~ctenumbtr T t I D__ Replicate nunter - Total Y 1 2 3 _4 5 6 7 1 8 9 110° l Yl I1 2 1 3 1 4 1 5 l6 1 7 1 a 9 I 10ll I 0 0 0 0 0 0 0 0 0 0 0 I, lo Ol O O 10 O 0 O IOl o Oo 2 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 -06 0 0 4 5 5 4 4 5 4 5 5 5 6 48 4 5 5 5 5 4 6 5 4 4 5 48 5 10 12 10 9 -11 I 10 10 12 9 104 5 1 11 111 13 12 12 1~l0 10 15 13 13 1l20 0 0 0 0 0 0 0 0 0 0 6 18 0 0 0 16 0 0 0 18 O 52
. 14 18 18 14 16 16 16 17 15 17 161 7 0 17 16 isI0 16 17 17 0 I 19 120 Totl 29 35 32 27 32 31 31 32 32 32 313 Total 34 33 34 35 1 32 321 32 1 36 35 1 37 340 10.98% 100%
Day 1 ReplIcate nunber Total Day Re lcate numberToa I1 2 3 1 4 15 7 8 9 10 1 2 3 1 415 6 7 1 8 9 10 I 0 0 0 0 0 0 0 0 O O 0 1 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 O 0 0 0 0 0 0 0
- 4 5 4 5 4 5 5 6 5 5 4S 4 6 5 S 6 4 5 5 6 5 5 52 5 Il I1 14 12 12 12 9 0 12 10 103 5 1 13 11 14 14 10 10 15 14 12 13 126 6 O O O O 0 0 0 11 0 I 6 0 18 0 0 00 0 20 19 19 76 7 18 1S 17 15 14 16 16 17 16 17 161 7 1 7 0 18 20 16 17 17 0 0 0 105 Total 33 31 35 32 30 33 30 34 33 32 323 Ttal 40 O363437301 321 371 40 36 37 359 22% Control-2 D Replicate nunber Total Day lRlilcte nunmer - Tehl r I 2 3 1 4 53 6 7 8 S 9 toe 1 2 3 4 5 6 7 8 9 1 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0O o O O 0O - 0 °0 O I 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 _ 0 0 °0 0° °0 0 0 0 0 4 5 5 5 5 4 .5 41 5 6 1 1 48 -4 4 3 5 4 3 5 5 4 4 4 41 5 13 11 12 10 I 10 11 12 12 141 121 117 5 11 11 9 0 10 10 11 9 10 12I 93 6 0 00 0 0 0 0 0 0 0 6 0 0 0 10I0 0 1I 0 0 0 01 10 1 7 15 19 161 16 14 17 14 16 16 181 161 7 14 717 1-5 14 14 15 13 17 16 141 149 Total 33 35 33 l31 l28# 33 30 33 36 34 326 Total V2 31 7 2V) 28 27 2 930 30 30 I 30 293 43.9% 100% Intake I
Day RReicate nunter l 21 i Dl RIcate nun-er Tet l I 1 2 3 4 5 6 7 1 8 T ta l l 1 221 3 4 l5 l d 7 l8 l9 l10l l I l 0- O I ° I o I oO~'-lo I o I l 0 0 O OO O O 0 2 0 0 0 0 O 0 0 0 0 0 0 2 0 0 0 0 0 0 0° 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 00 0 0 0 0 0 0 4 51 5 4 S 5 5 5 5 4 61 48 4 5 3 4 6 5 5 4 4 4 4 46 5- 1 13 1I 1 141 12 12 13 12 14- 13 13 127 5 I13 10 0 10 0 12 10 0 0 it 66-0 0 1 I 0 -- O O I 6 0 0 12 0 10 0 0 10 10 0 42 6
7 16 16714l9516l 162 7 I sl 15 13 16 17 15 17 15 16 1 18 157 1 Iotal 34 32 37 33 33 321 321 37 32 36 33 Total 133 30 2) 32 32 32 =2)3033 231 311 1
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA / Sequoyah Nuclear Plant, Outrall l01 - Non-treated Test dates: June 07-14,2005 Project number 1899 Reveiwed by: (7 /-_
Concentration Replicate number SurvIval Average reproduction coemckft of Pervnt reducton frm
(%) 1 2 3 4 5 6 7 l 9 10 (%) (oTsprng/emale) vartatlom ('%) pooled cortrol (%).
Control - I 29 35 32 27 32 31 31 32 32 32 100 31.3 6.7 Not appileable 10.98% 33 31 35 32 30 33 30 34 33 32 100 323 5.1 -3.2 22% 33 35 33 31 28 33 30 33 36 34 100 32.6 7.3 412 43.9% 34 32 37 33 33 32 32 37 32 36 100 33.8 6.2 -8.0 72% 34 33 34 35 32 32 32 36 35 37 100 34.0 5.2 -8.6 100% 36 34 37 40 30 32 37 40 36 37 100 35.9 8.9 -14.7 Control - 2 29 31 29 28 27 30 29 30 30 30 100 29.3 4.0 Not applieable 100% Intake 33 30 29 32 32 32 31 29. 30 33 100 31.1 4.9 -6.1 Outf2ll 101: MSD - Minimum Significant Diffecrence Dunnett's MSD value: 2.300 PMSD - Percent Minimum Significant Difference PMSD: 7.3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatuent that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when Intake: a toxicant reduces Ceriodaphnlareproduction by 9.4% from the control.
Dunnett's MSD value 1.050 Lower PMSD bound determined by USEPA (10' percentile) - 11%.
PMSD: 3.6 Upper PMSD bound determined by USEPA (90' percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USIPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Progm. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati. 011.
TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated June 07-14, 2005 Statistical Analyses C.erWd.ph. SorMv, %.dRerod.edor T.t.Rpredscdi
.Sta Dte: 6/7/21X5 Test Mo CdFRR SompleW. TVA/ Seqoyah NsckrPlant 101 - Non-trsted LnDDOe5: 6(14r.tD Lab M EMSEnvk.TestingSOL SampleType: DINt Disec Msie MO iReport Simple Date: Prnotocot FWCHR-EPA-Slt-R424013 Tert Speces: CD.Ccxalpmi dubi Comment,:
C{nc.% 1 2 3 4 S 6 7
- 9 1t D-Cootrol 29.000 35.000 32000 27.00 3'000 31.000 31.000 32000 32000 310DD 10.91 33.000 31000 33.000 32000 30000 33.000 30.000 34.OO 33.000 32000 22 33.000 35.000 33.000 31.000 21.000 33.D 30.000 33.000 36.000 34.000 439 34.000 32000 37.000 33.000 33.000 321000 1000 37.0O 3200D 36.000 72 34.000 33.000 34.000 35.000 3'000 32.0 3'0D0 36.00 3 35.000 37.000 200 36.000 34.000 37D0 40.000 30.000 32000 37.000 4COOD 36000 37.000 Traisft Ustnsfrmn.d I-T&iltd JlmIk C.C.% IMm NMs Mai Mb. Mis CV% N I&Stt telsl lSD M N-.m D-CogkoI 31.300 1.000 31.300 27.00D 35.OD 6.744 10 33.317 I.OODD 10.9 32300 1.0319 32.300 30.000 35.000 5.0D6 10 .994 2.217 2.300 33317 1.0000 22 32600 I.0415 32.6C0 2S.000 36.0 7.259 10 19M3 2.2n 2.300 33317 1.0000 439 33.SOO 1.0799 33.100 32.00 37.000 6.206 10 .2406 2.27 2300 31.317 . 1.0000 72 34.000 1.0163 34.000 32.000 37.000 3.11I ID .2.613 2.27 230D 33.317 1.00 100 35.90D 1.1470 35.900 30.0D 40.OOD t.l53 ID .4.574 2.2t7 2.30D 33317 1.0000 AwsillrT Test Sbtaitic Ctidsl Sk. Kurt rohoovDTest datesonmmaldmtutm(p>o 01) 0.741t21461 1.033 a.297616370.169024734 Bartlet's Tat Wind ta e1ad eunoes (p - 0.40) 5.125416756 15.0U627224 HythYsb Tet (I.6lO 9.0) NOW LOKC ON Tu MSDv MSDj MSI 6tSE FV-Pr.& O6 Dunreur Test 100 >100 I 2.299756327 0.073474643 25.97666667 3.057407407 6.3E-04 3.54 Treatments D-nontrol LrnSr Inastitt (200 Rnssnpl..)
Pl %' SD 95%CL Skn 105 >O00 100 >100 M102 >100 1.0 IC20 >100 0.1 IC23 >100 0.8 IC40 >100 C.7 IC0 >100 0.s Z 0.5 0.4 0.3 0.1 0.0 - - -
.. Is . ...........
@ °..° 0.2 0 20 40 50 so 100 120 Do. %
D".e4tep..se 7W 4: o 40 35 C30 iJ. 14all.0.06 hem oalgrlncame t25 10 0
10 5
I 2 v.
sqn_06.07-05data
TVA I Sequoyah Nuclear Plant, Intake Non-treated June 07-14, 2005 Statistical Analyses Cer.d.pkas. Strvivi s"d "~rederit.. Tes.kprodcelm StartDstes 6A1?705 Tat WD: CdFP9_R SamipklM.'VA/ Suoh NUCIM IRIntake -Non-trst EndDste 6/14/205 Lab IDl. ETSEnvk. TestingSol. Sample Tps: I)MR-Doda reMonkantg ptPCt Sample Du~e. rot3owt FWCHR-.EPA.821-R0)2.0I3 TaIt SpNviff CDCuiodzpbni dubia Case.% I 2 3 4 S 7 8 30 D-Co1okol 29000 31.000 29.ODO 23.0W 27.0W0 30000 290CtD MOD 30.0W0 30.000 too 33.000 30.0W 20000 32-.0D 32.000 32.000 31.0W 29.ODD 300WD 33.00W Came.% M;.. N4-A.eu Ms. Mia May CV% 14 %Stst Crd.'23 M.150 Naaa. N.M...
D)-Control 29.300 3.M0 20.300 27.0WD 31t.0W 3.937 30 30.200 8.0000 IO0 31.100 3.014 33.30 Z?.0W0 330OW 4.900 30 .2.97 21734 3.050 30.200 3.0w AnTI~eryTests sted'tk Qrik.1 Skew IKsr1 iprowoeWs Tast k~d~tts nonnal diorkution (p >0.01) 0.921665691 0.066 4034337024 .0.9042165 P-Tsstk d.3-e, utamvsr-=nc(p - 0*3) 1.727272749 6.541009533 Hypechls~s ag(114AU0.05) t MSDw PAS0p 065B 4S E F.Pr.& dlf Homoefdasts: ITestidicates Do sjgnifiostdiffertnces 1.0500267 0.035337122 16.2 1.033333333 0.000156700 1,10 Dyeat6eota Vs 0)Coftrol Lness..0s3plsesd. (200Ressampte)
FP41st 'A SD 95%C1 Skmw IC05 >100 IC15 >100 1.0 IC20 >300 0.8 1C40 >1000.
3C50 >100 0.7 3.6 30.3 0.2 0.1 0.0 ..... .
.0.
0 20 40 60 so 100 120 Dose %
- 1-tall, O.0Slevel otstgficakflce 25 120 10 S
8 I
MRn06.4-07.data
vnvironmental Testini! Solutions. Inc. Page I of 6 1'nvirflnhllental Testing Solutions. Inc. Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA County: Hamilton Facility: Seauovah Nuclear Plant Treatment: UV-treated NPDES #: TN 0026450 Outfall: 101 Project #: IOgA~
Dilution pre aration information: Comments:
Dilution prep (N) 10.98 1 22 43.9 72 1 00 Each concentration was treated Effluent volume (mL) 274.5 550 1097.5 1800 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2225.5 1950 1402.5 700 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 Interferences.
Test organism information: Test information:
Organism age: Z5 T j
-A.S oQauQS OQC Randomizing template: ApLL &_
Date and times organisms A0.-&o-O5 vb3O -m Vto Incubator number: _
were born between: .__
Organism source: PI&> RA~TA c--do-a Artemia lot number: , > r Transfer bowl information: pH Temperature = 'C Total drying time: -AcAS
_53 .* C- Date I Time in: 0iti-OS IMI5 Average transfer volume: IcO *o IQ Date / Time out: O'utSo-M i . IScZA
. . Oven temperature: ___C_
Dailyfeeding andrenewal information:
Day Date Morning Afternoon Test Initiation, hlHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 GS - 0 ccr i358 0ioO C)SO;abAo*.0 tt { t Pt O-S.r1 4 0Z.; -
I Ino-ttcQs toC0 iS52- 1Q6 1b2i t6-t4 A 2 6lo6-S Ocist i5SS IB0t C6E d cS C asoroa.L 14'3 B i 3 C4-l&C5 /000 t§S57 Jq4t ci06M-0S C. cOooI.zckz.-L A W A 4 0b-Ik5S ngm3- %15*0 13w20 cbd o6-S A, OSO" b.Gtz OB elk s 00 la-5 &S2. VASoC 135Z- Ob-08 IS Lo *o6
-am 03 6 OCISO S MS4 b 133;1 0&-OtO 6 OS0O .QZto 02 _2
__..._13... ___ _ s Control information: Acceptance critenia Summary of test endpoints:
%Mortality: O s 20% 7-day LC50 > IW7f Average weight per initial larvae: o.I1L, I .; J NOEC IM7O Average weight per surviving larvae: O.-11 2 025 mg/larvae LOEC )100%
ChV IC 2 5 > %
lvironnental Testing Solutions, Inc. Page 2 of 6 Species: Pimephalespromelas Date: o0.-oi-OS Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day CONTROL 10.98% 22%
A B C D E F G H I J K L 0
/0 /0 ID 10 10 /0 I0 IC) 10 lO /0 010 _-100/0 /0 0/10 _ t/0 10 2
D1 10 /0 10 10 10 10 JO to io IC 10 3
IC) t010 /00 1 01 1( IC)/0 /0 /o 6 0 t 10 /0 IC 0 /0 /o 10 /Io /0 to
_ _ _ 10 bo t to0 'o to Jr p A - Pan weight (mg)
Color Identification:
Analyst: f*66 B - Pan + Larvae weight Ao--
EMWA tq8 t qo
,1.
I4 I
1q 41 tC 1q4.90 i44.-s 1q.ri 'LAjs ,q.r'3 iN.li6Io5.
(kiqL1 Analyst: ,M 2. 2n.zl zz.zl zA2A 2+.50 z2.63 2z.i1 zt 22.15 z2.a2 zZAR 2I.qq I' Larvae weight (mg)
- A- B
.22. . I.SS (.OD .1v jq0 go.l2. -1.8& 1-10 1? 1..16.W 1.92- b6.R3 Ii Weight per Initial number of larvae (mg)
C / Initial number of larvae Sc k4 6 4~-
E 4,
I'
-- C'o - -' - n'0 - -
Average Percent weight per reduction I initial n from control 0.:1l 1.gl3 Li1 LIO1S'-'
II arvae me) of. 9 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.
II Calculations and data rcviewcd:
4r Comments:
Testingy Solutions. Inc. Page 3 of 6
,.,,ental nnta1 Testing Solutions. Inc. Page 3 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: o to1-o5 Survival and Growth Data Day Day -
43.9°/ - -----
72% -
100% -
M N 00 P Q R S T U V W X 0 'I IDiD-10 10 IC0 1/01 10
- 1 o1 o /O io) /O /O
//O O lIC)b/OIO l { / 10 /
t2 ID l IC) I* _lo IO 10 0 lo IC) 3_ _t o, lbIto ( I to 10 IC) Io to IC) iI(
1t) 10 10 10 /0 10 10
._ 1o0o to o 01010 lV I0.)IC ' IOt 6
1O lor /~_ to I Lr to ILW6 to t to 10 O 10 I10 IA /to I 0 10 to I A - Pan weight (mg)
Colorldentificstion:
Analyst:
6"WI -
v5Mo~bi~Sq.3I.I 151 51i,.51.3,,9 s ll.1 qqS lqq5 4.l,.S1,d2 l~l l...l,0 I B - Pan + Larvae weight (mg)
(mg) C -Y Z-2 25Th.-1M MIL 2Z.2lj \ 2Z.78 7>.z2 %z.'Z123-.z13 ZL22 Z3.1.2 2-z.(.. ZaZ4 I Larvae weight (mg) - A - B 8.87 8-* S.o 1. 6S R.e 7.77 RAi1 1.16 &.-I -1.l, 8.S;. -1v - .-27 Weight per Initial number _ _ _
of larvae (mg) 0 1 ~A C I Initial number orlarvae O O- O O O Average Percent weight per reduction .otI O7c 12 Initial from control 0.,i - 0.Oq O. -sq . 07 1. 8l _O51c number of (%)
Iarvae (mg)_.
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Page 4 of 6
. Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - 1W-treated Date: tb- C -S Survival and Growth Data Day 100% Intake Y Z IAA BB o 00 0It t I/O 1to 10 10_101 2 tO 10 /0 /
3 IC) lO 11 D 4 IC) IC) I lIO 5 1 01 /I00
____ _ _0//0/
A Pan welght (mg) /5 .
Color Identification: 6 14.20lq.10 15.l23 1.12-Analyst: .....
B - Pan + Larvae weight (me) 22 qO 23 13 232 Analyst: 41W_--A 214 .. 2 A2S 232Z, Larvae weight (mg) - A - B
. . 1.60 8AB1 9.07L gsk Weight per Initial number of larvae (mg) O'V V C /Initlal number of larvae *\0 Average Percent weight per reduction Initial from control O.8 number of (%)
larvae (mg).
Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:&
Comments:
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated June 07-14, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephatespromelas Quality Control VerifiCation Ot Data Entry, CalculationS and Statistical Analyses alkmt: TVA /Sequooyh Nulenul antOutfal 101. UV treated Test dates: lune07.14,2005 Project aumber. 2It" Rcelwed by: -I
__ __ Not for Compliance Asessment. [ntesnal .aborlory QC t(m'. t%3sl.8. 41 FII~w nl _ 8.rhe A - ft. .dzhI *-ts8. tL, t nl*e m1 () Wsi:8 I8ntd ~65. .MIR C _d .4 -vl-. wuh IsI4e3 lu8 t --- t -w*s r St.
Fh dgbd C..d.t. hron .Wa.
t a* t)h (A) bnw"A- t -. d -_ I__
_ s 9l iilu A5 @edvfy ett _ bsfl 1 lb~ vlr_ t ta A I0 t0 34.98 23.20 8.22 0.822 0.822 Control B 20 to 14.69 22.21 755 0.750 0.776 4.0 0.7t2 100.0 0.776 4.0 Not applicable C 30 20 14.61 22.21 7.60 0.760 7.76 sD to 10 14.80 22.48 7.6t 0.76t 0.765 E to to 14.90 24B0 9.40 0.940 0.940 10.9t% F 10 tO 14.51 7.12 22.63 0.72 0.8t13 13.8 0.725 100.0 0.513 11.8 -t47 G I30 30 24.89 22.77 7.88 0.788 0.728
___3 30 30 14.98 22.08 7.10 0.710 0.710 3
10 30 14.83 22.75 7.92 0.792 0.792 22% K 30 30 14.70 2232 7.62 0.762 0.755 6.5 0.762 I00.0 0.755 6.5 2.8 K I0 I30 135.16 22.98 7.82 0.782 0.782 L 30 20 14.66 21.49 6.83 0.683 0.683 M 9 10 15.16 23.98 8.82 0.980 0.882 43.9% N t0 to 4.72 23.71 8t.9 0.99 0.874 10.2 0So 97.5 0.849 7.0 -9.4 0 30 30 14.73 23.23 8.50 0.850 0.850
_p I30 20 15.16 22.81 7.65 0.765 0.765 30 S0 I5.01 22.78 7.7? 0.777 0.777 72% 30 30 14.75 23.22 8.47 0.847 0.8tO98.2 .O847 I00.0 0J09 8.3 -4.2 S 30 30 14.93 22.27 734 0.734 0.734 T t0 30 14.95 23.73 8.78 0.878 0.87t U t0 30 14.61 2218 7.67 0.767 0.767 Il% V tO 20 t4.66 2322 S.66 0.866 0.781 7.8 0866 300.0 0.781 7.8 -0.5 W 30 30 35.00 22.67 7.67 0.767 0.767 X t0 t0 1522 22.44 712 0.722 0.722 30 34.80 22.40 7.60 0.760 0.760 Io-r. Intsae Z 30 20 24.70 23.13 8.43 0 843 0.839 7.0 0.942 300.0 0.839 7.0 .8.3 A_ 30 30 5.723 2425 9.02 0.92 O.9S2 all0 30 30 34.72 23.23 8.53I 0.851 _ _ _ _ _ _ _ _ _ _ _ _ _ _ 0.853 _ _ _ _ _ _ _ _ _ __ _ _ _ _ _ _ _ _ _
Outrall 01 MSD - Miniamumn Significant Difference Dusustels MSD value: 0.e079 PMSD - Percent Minimunm Significant Difference rMSD: 33.9 PMSD Isa measure oftest precision. The PMSD isthe min~lmm percent difterence between the control and treatnent that can be deelared statlstically signifieant na whole effluent toxicity test. On averige. a significant difference occurs tor Environmental Testing Solutions. Ine. chronic toxicity tests vhen a toxicant seduces PFmephattegrowth by 14.0% onsthe control(determined through Intake: ecteeence toxicant testing).
Dunnrtl's MSD value 0.0646 rwerPMSD bound determined byUSEPA (Ihth percentile) - 9.4%.
PhtSD: 83 Upper PMSD bowod determined by USEPA (90th percentile) - 35%.
The lower uadupper bounds were calculated by the USEPA usin g 205 tests conducted tono19laboratoriet fore RFepksfes growth in chronIC teftrence toxicant tests.
USEPA. 2000. Undestundingand Accounting for Method Vaiabiy a Whole Emunit ToxciyAppGcatos Undfthe National Polutant Discharge Elmiuatio Prognm. TEPA-833-R00003. US EnvironmentaI Potection Agency.Cincnat. 011.
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated June 07-14, 2005 Statistical Analyses LArmFb Cra. sad SurvIal Teut.7 DayCrwwt S3taDat: 6tnos Test ID. ppRCR Sample ID: TVA/ Sequoyth Nuclear Plent 101- LNVdeated Eod DaL: E14=5 Lab lD EFE. TaS6g sol Sample 7yc: DI)MR-Dichare Mfaltodft RPood Sample Dbt: Protocol FWCHR.EPA-21-R424-13 Test specl: pr-.Fmerphale promela C .Y. I 1 3 4 DQConfrol 0.22D 01750 0.7600 0.760 10.93 0940 0.3120 0.71130 0.1200 22 07920 0.7620 0.7120 0.6t30 43.9 0.1120 099 0.1500 07530 72 0.770 0.470 0.7)40 01730 100 0.7670 0.3660 0.7670 0r.20 Tranifrm: Uat -farvaom I3Tailed I,4.-le C.e.% Me-. N-Mea. Ma. Mh Me l CVY. N -Stt Ctltket MSD Me. t-;4tl D.Cou &7763 1.O00 0.7763 .7550 n.220 3.939 4 0.303M 1OCOC J5093 0.3123 1.0467 0l7.35 07100 QS400 11.754 4 .0.t10 2410 0.079 0.ntoo3 I0O 22 0.7543 ar9723 0.7348 630 0.7920 6.550 4 04,10 2.410 0.3079 0.$303 3.ox 43.9 0.8490 1.0937 0.890 0.7650 o.m0 7.017 4 .1.625 2430 0.3079 0.30X3 1.ODO 7n o.3090 .04 0.090 0.7340 0.m70 3.091 4 nn71 2.410 0.2079 0.303 1.OXD 100 (15 .0033 0.7903 0.7220 .t660 7.792 4 4095 2.410 m.079 0.7CS 0.9753 AdyTt Stetle cOt.l Skew Kurt Sbapwk*.Wr Tadt data normal daVmtNa (p > 0.01) 0.93221791I ntu4 0.n34096212 .0.0596U54 Bufftg Teat kdlatta equa varinces (p -0 65) 3.292W4too 150S677224 kly(ptealu T eat (I.&II, 0.03) NOEC LOW OV TU MSD MSDp MSB MSE FY.a C DuatsaTeat 100 >100 I ot07903775o0139(064730.0044609 0.004009306 0383406605 5.LB atment YeD-04eCol Lheor lutaala(200 Ittoo aanples)
PW. SD 95%.CKep) Stew ICOS >100 ICIO >100 ICIS >100 1.0 C20 OD 0.9 1023 >100 .0.3 IC40 >100 0.7 2030 >300 0.6 0.5 0.2 0.2 0..
.0.1
.0.2 a 20 40 60 s0 100 120 D %
Dw"Rw"*eP.W 0.3 0.7
.. . . .. . . . .. . . .. . . . 14al. 0.05 level C .? d sgriflcarnce I0.6.
es O 0 0 0.4 VW 0.3 0.2 0.1 N
9: p 6
sqnO607.OSdata
Environmental Testing Solutions, Inc.
Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalesprotnelas Daily Chemical Analyses Client: TVA / Sequoyah Nuclear Plant, Non-treated Test dates: June 01-14, 2005 Project number. 199 Y
Reiwdb-,' I Concentration I Parameter Dav 2 Dav 3 l Dav 4 l DayvS Day 6 4 4 Day0- Initla I _lnal Initial I Final I Initial I Final l InitIal l FInal initial I hi
.4 ntal
.4-
,1II (Sill Inithl 7.651 7.532 7.68 7.42 7.851 7.671 7.721 7.701 DO (mZ/L) 7.8 7.4 7.8 7.9 7.8 7.5 7.6 7.5 2onducetvitv (umhonen) s is r 4-heI z 308 2 94 _ 303 297_
Control 58 Alkalinity (mr/L CaCOA) 62_
4- _82 Ilardness (mg/L CaCO,) 80_
Temperature (C) l .4.YI Z4.YI 4a.UI 24.8 24.6 24.8 24.5 l - 4--
All (SU) 4-7.89 7.75 7.97 7.61 7.84 7.60 7.73 7.43 10.9R%
3O (m/DL) - - - .8.11 7.41 8.1 7.51. 8.0 7.3 7.6 7.6
-onductfvftv (umho: ml Codutiit(im4-¢m I 287Jiu1 285 Ji~ 290 - _ 276i
_Temperature(C) 25.3 24.6 25.01 24.3 24.81 25.1 25.1 25.11 25.1 24.6 249 24.5 24.7 24.5 pil (StJ) 7.88 7.66 7.83 7.59 7.87 7.78 7.96 7.621 7.77 7.68 7.84 7.61 7.72 7.45 22% DO(mg/L) 7.6 7.8 8.0 7.3 8.1 7.3 8.1 8.2 7. 80 7-4 7.6 7.7 Conductivity pmhos/cm) 273 _ _ 276 _27 274 271 _ _ 28t0 _ 268 Temperature (C) 25.2 24.6 25.0 24.5 24.9 24.8 25.1 25.1 25.0 24.7 25.1 24.3 24.7 24.5 piI (SU) 7.85 7.64 7.80 7.60 7.83 7.83 7.95 7.62 7.74 7.68 7.83 7.63 7.71 7.49 43.9% DO (mg/L) 7.6 7.5 8.0 7.3 8.2 7.4 8.0 7.4 8.2 7.8 8.0 7.3 7.7 79 ConductivIty (umhos/em) 248 255 2521 253 248 258 245 Temperature (C) 25.2 24.8 25.11 24.4 24.9 24.8 25.0 24.9 25.0 24.7 25.1 24.5 24.8 24.5 PI(SU) 7.1j 23e 7.69 7.80 7.64 7.81 7.85 7.92 7.63 7.70 7.71 7.81 7.61 7.70 7.50 7t% DO (mg/L) 7.6 7.7 8.0 7.3 8.1 7.4 8.0 7.5 8.4 7.7 8.0 7.4 8.0 8.3 Conductivity (,mhostcm) 227 228 _1 222 223 220 226 211 Temperature (C) 25.0 24.6 25.0 24.5 24.7 24.8 25.2 25.1 24.9 24.6 25.1 24.4 24.7 24.3 PI. (SU) 7.78 7.68 7.79 7.651 7.79 7.83 7.91 7.63 7.65 7.74 7.78 7.641 7.69 7.57 DO (mWL) 7.6 7.81 8.0 7.4 8.2 7.3 7.9 7.6 8.2 7.7 8.0 7.6 7.8 8.3 Conductivity (jmhoslcm) 197 202 194 192 189 19! 183 100% Alkalinity (mgfL CaCO)7 71 71 I ardness (mg/L CaCO,) 80 80C Total Residual Chlorine (mgfL) <0.10 .10 <O.I0 Temperiture CC) 25.3 24.7 25.0 24.5 24.7 25.0 25.21 24.9 24.9 24.7 25.1 24.4 24.7 24.4 p11 (SU) 7.90 7.66 7.85 7.68 7.87 7.89 7.95 7.68 7.88 7.74 7.84 7.67 7.70 7.57 DO (mgrL) 7.6 7.8 8.1 7.3 8.2 7.5 7.9 7.5 8.5 7.8 8.1 7.6 8.0 8.3 Conductivity (mhoslcm) 196 201 194 192 189 19' 180 100% Intake Alkalinity (mgtL CaCO,) 74 75 73 llardness (m/L CaCO) 88 71 78 Total Residual Chlorine (mg/L) <0.10
<0.10 <0.10 Temperature (C) 25.3 24.61 24.8 24.5 24.9 24.8 25.2 24.9 25.0 24.5 25.21 24.5 24.9 24.4
Species: Pimephalespromelas Date: O%.- - G5'S Client: TVA / Sequoyah Nuclear Plant - Non-treated flitkv Chemistr":
. Dav
.0 . I2 Analyst Concentration Parameter AW WlGi CONTROL pH (S.U.) es_____ ______ _ _1_-U1 DO(mg/L) ,<1 ).6 8.O Conductivity 500S (gmhos/cm)
Alkalinity (mg CaCO/L) 101 Hardness (mg CaCO1 /L)A Temperature(°C) 2s- Z..'4S zt. 8 21.lb.4. A pH (S.U.) +.t IaCl 10.98% DO (mglL) _1___ _1_
Conductivity 2'5e _________
(Amhos/cm) 2 _____ 02q Temperature (C) 2S.B zq.1 7 -24.
2S Z1 pH (S.U.) IOS.e? - l 22% DO (mglL) _4AC n 01 -7 a-Conductivity Z43 ?.to Temperature (C) ZSZ mg.lo 25.0 l. 4's.5 ~.q l .8 pH (S.U.) - _A __ .Eo. , -
3-8 3 203 43.9% DO (mgtL) _I.0 1 -7 Conductivity zq_
1(nimhostcm)_____
Temperature (C) _ S';Lq Z 2'Z* 1i(. l .4.
pH (S.U.)
72% DO (mg/L) _p_ . A.0__ _.5 _ ._
Conductivity Z (pmhos/cm) IZ 3+ -2Z&
Temperature (°C) IS.0 pH (S.U.) _ _-4.-+
100% DO (mglL) - O Conductivity 1 4,G.7 (ymhostcm)
Alkalinity : nt (mg CaCOtL) 1t Hardness 4 (mg CaCOL) SC)
TR chlorine '-0.10 Temperature (SC) s. - . 29n LI S pH (S.U.) 4O I ,_-: ).s -4.0 100% Intake DO (mg/L) 4;(a Conductivity 1 (grmhos/cm)
Alkalinity (mg CaCOA/L) 19° 7
is I Hardness (mg CaCOAL)
TR chlorine (mg/L) (0C . . I e40.10 Temperature (°C) ZkS-.3 _
Initial I Flnal I Initial I Final IInitial Final Initial Final I Initial I Final I Initial P
Page6of6 F; viro nmental Testing Solutions, Inc.
.. Species: Pimephalespromelas Date: tM-dC-OS
., Client: TVA / Sequoyah Nuclear Plaint - Non-treated Day
.. -. A S 6 Analyst KC-, Ib~ V)PN _ XKsAI AC it Concen- Parameter I.I I
.-: CO.NTROL pH (S.U.)
DO (mg/L)
Conductivity zoo
_.4 .
".X q t3 7.10 .
7 7
.. 5
-7..
.8 Ii (mg CaCO1 /L)
Hardness (mg CaCO3/L)
Temperature (C) V A .°l Z . P*____8 __ ___.D pH (S.U.) 3 2CC 7. f_ c O I
- _. _.-I 10.98% -DO (mg/L) 0.1 9 5 010 7- 3 7. L- 7 C Conductivity 2835 22 q 27
.(pmhos/cm) _ _ 5_______
Temperature (°C) 25.S 7I Z . _ _ Is 71 i .5 pH (S.U.) 4.91 4AO7
- 6 L 1.6t 4.l__
22% DO (mg/L) 0. I Conductivity . qq
( nimhos/cm)__ _ _ __ _ _ _
Temperature CC) 35j 5-1 LS.Q -j - L'. R4.1 ?A.$.
pH (S.U.) -6 -7
- 1. jX 1.1.3 1.V Z 43.9% DO (mg/L) . 6A. S 8. 0 7.
Conductivity ZS3 2Sd ____
(TMhos/cm) Z5
____Temperature (C) I .O 2'A j qSQ -ZS A8 V4. '
pH (S.U.) -40i2 -7.11 1.61 -1.1r) 72% DO (mg/L)
Conductivity (pim hos/cmn)
Temnerature (°C) 8 Z Z3 25.7-
-is.
I I__
a.
22zo
'i.S I-.
1I___
S*
0-7.
2ZILD 7 4 V)/ /
O 3
L -3 4 - -- -. 4 --- 0 §7 = 4 41 I.
- 41 4 41 I' pH (S.U.) -4.*O1 -7.7+ 1-. L 71. V 444. Slit 100*/0 DO (mg/L) -.1 Conductivity
_ ~. . I -_._t (Amhos/cm) lc0, Alkalinity (mg CaCO/L) _ L 1[I 1 Hardness (mg CaCO/L) 8 -
Temperature (C) Z2S 2.4.¶ 4 7ZS. I Z4A Z.-1 24 .Z(
pH(S.U.) t.9 . .D 4 I .7 8 7- 1 i .10.1 100% DO (mg/L) . gf 7*4 Intake __ _ C)__ 7S'.'
Conductivity 19z1 80 rnmhoslcm)ec Alkalinity (mg CaCO/L) 13M Hardness MARw (mg CaCO,/U TR cblorine (mglL) _ .I OC)O Temperature (C) 2S 2Y .9 - ZS - l7 7__
Initial II Final I Initial II Final Initial I Final I Initial I- - Final I
-
- 6 U - S
- S 5
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphitiadubia Daily Chemical Analyses Clitnt: TVA / Sequoyah Nuclear Plant, Non-treated Test dates: June 07-14, 2005 Project number: 18"9 Concentration Parameter Day 0 Initial l pIH (SU)
DO (mrJL)
Conductivity (pmhostem)
Control Alkalinity (meIL CaCO)
-. 4-Hardness (mg/L. CaCO3) remnerature (C)-. , 81 nil ISI I 4-DO (mg/L) _,.: _ i ..o.,,__. =II-8.21 8.11 _
8I I
~~w~~i R8 8. 7. 8.0 10.98% Conductivity (umhnsteml wm miv- __L~~
\ w-
... I.
Temperature (C) 24.61 25.21 24.8 I 25.0 24.61 25.1I 24. 25.: 24.51 24. 24. 25. 24. 25.1 pit (SU) 7.88 7.73 7.83 7.77 7.87 7.89 7.96 7.781 7.77 7.77 7.84 7.66 7.72 7.6i 22% DO(mg/L) 7.6 7.8 8.0 8.0 8.1 80 8.1 82 8.2 8.0 8.0 8.0 7.6 8.1 Conductivity (pmhoitkm) 273 276 277 274 271 280 268 Temperature (OC) 24.7 25.2 24.8 25.0 24.6 25.1 24.9 25.1 24.7 24.8 24.7 25.2 24.8 25.I pi (SU) 7.85 7.74 7.80 7.74 7.83 7.89 7.95 7.79 7.74 7.77 7.83 7.66 7.71 76 DO (nJL) 7.6 7.9 8.0 8.0 8.2 8.0 8.0 8.2 8.2 8.0 8.0 8.1 7.7 B."
- Conductivity (Gimhos/cm) 255 253 248 '22 248 258 245
_Temperature (C) 24.8 25.0 24.8 24.9 24.7 25.2 25.1 25.2 24.7 25.0 24.9 25.2 24.9 25.1 nit IqII1 7_831 7-7 1893( 7.74 7.8 II 7-9tJ II 7.921 7.79 7.701 7.7 7.XIi 7.6' 7.7( 7.6 30 (mg/L) 7.6 7.91 8VA 7.9 8.; 7. 8.0 8.i1 8.4 8.C0 8.01 8.2 8.0 8.
72% Conductivity (umhos/cm) 1 227 _m 221 2 22 14 _ 223 II 220 226 211 Tempernture (Co pll (SU) ' 7.76 7.79 7.79 7.79 7.79 7.65 7.79 DO (mnWL) 8.0 8.0 8.0 8.2 8.2 8.2 8.0 Conductivity (pmhos/cm) _ 20;194 189.
100% Alkalinity (mg/L CaCO3) 71 I lardness (mg/L CaCO,) _ 80 80 tat Residual Chlorine (mg/L) <I _11<0.1(0I <0.10 Temperature (C) I 25.11 24.812S.3124.91 25.2 25.1 25.0 pil (SU) 7.81 7.88 7.79 DO (mEgt.)
-7.61 7.91 8.1.1 8.21 7.9_ 8.0 8.5 8.0 Conductivity
_ __ , (umhos/cm)
,_ _ _ . , 196 _ 201 194 189.
100% Intake Alkalinity (nV)L CaCO,) 74 75 73 11ardness (mgtL CaCO,) 88 7 78 4.-
Total
_ Residual\ Chlorine (me/L1 w 24.10 <0.10 <0.10 Temperature (C) 24.81 25.11 25.0 2. 25.2 24.7 24.8
Environmental TestigSltons, Inc. Page 6 of 7 Environmental Testing Solutions, Inc. Page 6 of 7 Species: Ceriodaphniadubia Date: C1b-01-OS_
Client: Sequovah Nuclear Plant - Non-treated Daily Chemistry:
Dav Anayt '
Concentration Parameter CONTROL pH (S.U.) ___1__
DO (mg/L) + L9 w Bl IaO w Conductivity Nimhos/cm)
Alkalinity (me CaCOL) to3 Hardness (mg CaCOt5L)
_______ Temperature (C) 1.L. 2.1 A .. 1 2.
pH (S.U.) 3;-4 4.8q 10.98% DO (m/iL) .- I _ .6 Conductivity (Amhos/cm)
Temperature (C) 2.Iq.e r;7 - L .~
H (S.U.) ' _ _4._4' 22% DO (mg/L) d i
. Conductivity
(
(;imhos/cm) 5 . Om 7Z7 ZW 1 Temperature (C) 2W .1 L. 12Yg- E 0 IV (.
pH (S.U.) ;r TF w.S 4.0e:, * .
43.9% DO (mglL) Theo _ toi _o 6.s .
Conductivity (umhostcm) 2L40 l 55--5 Temperature (C) _
pH (S.U.) 1_ ScD
_4-0 7e1
-40 72% DO (maL)__----_-
_ .,o (3q
,1 ;.q, Conductivity (umhos/cm) '2ZI x, ~~.... I wze 22z\ .i ~
Temnerature MC) pH (S.U.) +.3 IP n-qs~ IL4.J 7-S
- 100% DO (mglL) .IO :1 Conductivity _ I_ OzT (pmhos/cm) Pq Alkalinity -14 (mg CaCO3JL) -71 Hardness (mg CaCO3/L)
&O
-C0-to
-':=: -5 Temperature °CC) IZi. _ 6z. I
-4.9 42 s DH (S.U.)
4.-
1 :t.-iq 100% Intake DO (mgL)
Conductivity
_-AI_4.
-I--___ ?eq r H (pimhos/cm) I9 t 201 Alkalinity -7q iRP5 (mg CaCOIL)
Hardness -it E (mg CaCOJIL) o TR chlorine 4010 Sg (mgIL) 0 0
- 24. & 1 24.1 I2.
I Temperature
(°C) I I I Zs. iI initila i Final I_ i t Final F Initiai Final
E~nvironmental Testing Solutions, Inc. Page 7 of 7 Environmental Testing Solutions, Inc. Page 7 of 7 Species: Ceriodaphniadubia Client: Sequoyah Nuclear Plant - Non-treated Date: 06-61-OS Dsv 4 %5 _ 6- -
I *Analvst ik- I siy 19 14J- 1 li_ I 4:YW Y A I AA 11f Cooce nw Parameter tratlono_ -
1 7.-7'1 CONTROL DO (mgJL)
Conductivity
-.4_PV q- 1?
n- I 1.65 I I
1 .X7 I
ii I I (prnhos/cmn) e Alkalinity (mg CaCOI/L) - l _ S 8 SDgo Hardness (mg CaCOAL) a 28 Temperature (°C) 25. I Z .1. 2s.3 7zL .-I t I -' --
nH (S.U.) -1.? Ia7. 4 1A.-!a .6 10.98% DO (mg/L) I It .2 __ _ . .
Conductivity I _ 7s 7AW
-53 IS. 6 (gpmhos/cm) I- 5 I
Temperature (°C)
I*
ZM .9 25.3 9 -r-- 9 -)-%A .-s 0-pH (S.U.) -I .S(Ao 1.12-.
22% DO (m!/Ll A 1 4 .0 -l0 Conductivity (Prnboslctn1)
Temperature (°C) 7 -I I-Zi.M2 2-4
-_TI E
-- .-I 20 43.9%
pH (S.U.) *.
-DO (mg/L) I C1 B ..
1h: .Li 7. It :*
L2 (umhos/cm) ZS3 243 _E_2Y Temperature (°C) Zs. I -7A.2S 25.0 3 2j pH (S.U.) 3.9q 72% DO (mv/L)
_ _ a-, _, - . _wl u Conductivity (umhos/crn) .. 2ZO Temperature (°C) 24-.
- 0 *- -
pH (S.U.)
r-- o 100% DO (mg/L)_ I v
- 1 0,2- .0 Q.SD I
- I_
Conductivity (umhos/cm) 109 I5,.
1i q x
Alkalinity (mg CaCO,/L)
Hardness (me x _CaCOJIL)
Temperature (MC 40.10_~~~~~V _ 5.-. Z.
pH (S.U.) RE;) 759 1 qq 5.
100% DO (mg/L)
Intake -:~q I0.-0 0.5 g.,a 1 P.oI Conductivity (Pmhos.cm) 19 Il9 l&o Alkalinity (mg CaCOJ/L) .13 Hardness (mg CaCOJ/L) I &9 TR chlorlne (mg/L.) m! 7 l Temnperature (°C) 75. U 27.TL S,.0 CZ -. t' -. Z _
I
--- 1
. 4- -.
I I initial Initial II Final Final II Initlal InitIal II Final Final Initlal I Initial II Flnal Final II Initial I Initial
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client: TVA/Sequoyah NUCear Plant, 1W-Treated Test dates: June 07.14, 2005 Project number: 1899 Reviewedby;_
Concentration Parameter l)a 0 Day I Va 2 Da 3 Day 4 Day 5 V 6
________________________ Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pit (SU) 7.86 7.64 7.76 7.63 7.80 7.73 7.90 7.57 7.81 7.74 7.82 7.53 7.68 7.50 Control DO (mzJL) 7.7 7.7 7.9 7.5 8.2 7.2 7.8 7.3 8.1 7.9 8.2 7.1 7.7 7.4 Conductivity (pmho-Jkm) 2430.9 299.2.86 295 2196
________Temperature (OC) 25.2 24.7 24.9 24.8 24.9 25.1 25.0 24.9 25.1 24.6 24.7 24.3. 24.8 24.4 pit (SU) 7.86 7.64 7.76 7.65 7.79 7.71 7.91 7.57 7.79 7.70 7.82 7.60 7.71 75 109% DO (mzfL) 7.6 77 8.2 7.5 8.2 7.1 7.7 7.3 B.1. 7.9 8.21 7.2 7.77.
109% Conductivity (pmhios/cm) 28'4 293 288 28'4 282 292 2'
________Temperature ("C) 252 2. 25.2 24.6 48 24 24.9 24.9 25.0 2. 24.91 24.6 25.0 2.
pi1 (SU) 7.85 7.4 7.76 7.4 7.79 7.78 7.91 7.8 7.76 7.5 7.81 7.59 7.71 75 22% DO (mzfL) 7.7 78 8.1 75 8.2 74 7.9 728.1 . 788.3 7.2 7.672 Conductivity (pmhoslcm)25 282 276 27/5 272 28174
________Temperature ('C) 25.3 24.6 25.21 24.6 24.8 2 25.1 25.0 25.0 462524. 25.0 2.
nil (SU) 7.85 7.63 7.76 7.61 7.79 77 7.91 7.60 7.74 7.69 7.81 7.8 7.71 75 49% DO (zL) 7.6 7.5 8.1 7.4 8.1 73 7.8 7.5 8.2 7.8 8.3 737.874 Conductivity (pmlioslcm) 263 261 253 253 249 29251 Temperature (C) 25.4 24.9 25.1 24-9 24.8 251 22 24.9 25.2 24.6 25.2 24.31 24 24. 3 pH (SU) 7.84 7 62 7.77 7.67 7.77 775 91 7.57 7.72 7.68 7.80 7.60 7.71 75 72% DO (mz/L) 7.7 7.7 8.2 74 81 71 78 7.4 8.2 7.8 8.4 7.2 - 78 7 Conductivity fpmnhos/cm) 226 232 22625 221 ~ 227 221 Temersture(C 25.2 24 25.2 248 29 251 23 25.1 2501 24.4 25.1 24.4 24.9 24 PI[ (SU) 7.82 77 7.75 769 75 7.7 791 7.61 7.68 7.76 7.79 7.61 7.71 7.50 10% DO (mgIL) 7.7 7 8.2 74 82 7.1 9 7.3 8.1 7.8 8.3 7.4 7.9 7.4 Co0%nductivity (tzmhosjcm) 16208 197 --- 194 196 1(.I9 Tmperxture CC) 25.2 24.8 25.1 2481 24.9 251 22 25.3 25.1 24.7 25.2 24 25.1 24.5 pit (SU) 7.90 7.64 7.77 7.651 7.79 783 93 7.62 7.80 7711 7.82 76 7.73 7.51 100% Intake DO (mnL) 7.8 7.7 8.2 7.31 831 751 8.0 7.3 8.2 781 8.4 742 3 Conductivity (_umhoskcm) 198 ~ 2?!F 195 PEI"191 189 194 11
_______Temperature (9C) 25.3 24.8 25.1 2481 2481 25.11 25.4 25.1 25.11 2471 25.3 242501 2415
al Testing Solutions, Inc. e SPage 5 of 6 A' Species: Pimephalespromelas Date: 06 OS
- ...... Client: TVA / Sequoyah Nuclear Plant - UV-treated
", .fl%
DailY Chemistrp:
Day 0 1 2
-"--I' Concentration Parameter CONTROL pH (S.U.) . 4_30 l *eo S DO (mg/L) ) . 1 5 1ttT7 Conductivity
+/-m hos/c m)_
zq0 t.*, -
(p _ _ _ _ _ _ _ _ _ _ _ _
Temperature (C) 7S.2. l . I ts.%
p1l (S.U.) . 77G3 - J 39 ?
10.98% DO (mg/L) .to *.0 1.2
- .'- . Conductivity z&e4 (RTmheslcmt) in7__2_
Temperature (C) 'ZS.2.3 *.1 2Sb 7.l ?-1.(o ZS pH (S.U.) __-___1__
24% DO (mgfL) 15l2 ,1 Conductivity Temperature CC) -Zs. 2.t 2. 1 2 ZL a -Zs .l 43.9% DO (mgfL) / X.13.? ell Conductivity 1(5212 pH (S.U-) 4403 --II- n-to S-4Du i t.Tq Fy Temperature (C) 2S.Z 2'4-4. 25.1 'R 2I.2 1.S.1
.(gmhos/cm) 2Zt 3 72% DO (mg/L)
I i. Conductivity pH (S.U.)
Temperature (C) 2 Z Z 2 it E.
5Ak?
- 5
'Z 9 9
-. R I. 1 I. 100%
(umhoslcm)
DO (mgi/L)
Conductivity tq q
_.3 0
-DZof 14 7;
I I (iunhom uslcimi)
C oiti Temperature ( C) 0 19~0 altF 3 i nal 2t___1___
I Ijl S q9
_ _l nj Initial Final . -
Initial . Final . -
Initial - -
Final
I restinty Solutions. Inc. Page 6of 6 Testing Solutions, Inc. Page6of6
! Species: Pimephalespromelas Date: 06-01-gL
. Client: TVA I Sequoyah Nuclear Plant - UV-treated i, . ;;.:-
0-Day
- I - - ,. - - - -
- !. - , , -Z
.h t i - I . F t ¶1 I ** I Analyst 1~tk I I1i- tjnq. A --
I Parameter
. LOil'KiL nUT (S-U.) 4 or -7.74.
DO (mrgL) . . 3S. ) _j I .9 __.__ / . 7 7 .7.7 e'sk ', -' i-ConductIvity I 2ex.0 I ____ i28 )..-
- 1. . .. ., .
.:. - ' ' . :' Temperature (°C)
. -. 1- .
24.9 2-S.I Z%4.i .-J I -Z4.- .zq.q
- 14. pH (S.U.) -.10 .to0 I -1.-I DO (mgiL) l .J . 1 1 1
- 8 a t 7.7 1 _
Conductivity (p+/-mhoslcm) .1' .M 2aZ hL~ 1 -J lcpi> --
Temoerature IC) 7-4. Lw.L Zs.
i .S0 I
pH (S.U.) -4.?tw 7. 45 17. b'
- ' 22% D0 (m-/L) _ -'ii9 I
- S.;',..
Conductivity Z-1
- _____s -
Ttmperature (°C) -LS I lS.° Ls .0 25.0 P
!I - Y F I! -Y -. ii P pH (S.U.) -7."' -.l5W 9 .- I 14
. 43.9% DO (mglL) ij 8 4 V- i .-. - 1,5
- ;I -:
I5, (gmhos/cm) 7265 2Lfq ZE___
Temperature (°C) 24.q 2'I .3 I-I -
- _*~~-- A g'~'- 4 _~~ _ _- I? _ _ 4_- _ ___
pH (S.U.) 1.69 ,AaO ' .- I
': 72% DO (mgL) q. J; Conductivity _-
I Z 51~2 nf - - - - -J -.
~ aI--,,--.
~ ~J II ..
(gimhos/cmn) I L2 WJ 0z ifl 2z. O IcyP'QI -
Temperature (°C) ZS I
____ __ /, JL
-S.0 2Z .4 2L3 °l pH (S.U.) - -4.9 I _ , __ _ -7. 1 i - .1 t 1k _7.
100% 1.3 i.-
DO(mg/L)
Conductivity
_lmhoslcm ) -
.cm9 i1-4.S.Z -
yr qw r, 41 S -
0.1 o9 r 7 3
Tempoerature (°C) - 2S Z. _Z- 7-St % _4 ZS. -t a ist2. I Zzq.s pH (S.U.) 1.11 -1.La' I I *-10n 3 100% DO (mg/L) 8-0 *;Z..: 3 B.Z i- 1 .Y 7 + 7. 3 Intake Conductivity 1 c 8.1 i i Temner2turp (°C'l 12S 1 7q.
- t. 9 O.- 5-I
,dI - -- 0 9-,-§ - - I---
12"c -
nX . - -
Initial -
Final . n Initial * -
Final S -
Initial a Final .
I Initial -
Final
Environmental Testing Solutions. Inc.
Page 4_ _
Page_Lof I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0. 10 mg/L Meter Accumet Model AR25 pH/Ion Meter Analyst I ki-Y I l1 Iodidereagent: lIjiR l q Date analyzed I Acid reagent: l I ba Db.0-4-o5 Calibration:
i-i .1U0 m /L l 1.00 m=/
! Reference standard number I IW 2 I t4SSaO I Note: For samples with a residual chlorine of > 1.0 mg'L the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (NIV) % RS =MV /TV x 100 number (mgIL) t(meL) (acceptable range - 90 to 110%)
/ IT) 0.50 O 614b lQE51 Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD - U(S - D) I[(S+D)121) x 100 number ____ _ (acceptable range - *10%) l C0S5bW.,Oil _C,
- OD -b,d- S /-O 06Zq I ft rL Duplicate , D U 33l - l Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine
- number (mg/L)
Blank (shouldbix<D.10mgrL) 1' A O. q 2.
"0 coC*o1.O2.. fzc - 10 OOC.QOg., C.AiA _________
oSoI-1,c3 Oorv A P 4, p- -c-O UZ 9Z osbsa o0q Q,;tvb ahkU- w tno L10 Ctlle 4- COO %09C£ 0 06LOpb-oL 1A-StokJ foIg" , ckaa O.O O5aco.o _oI lUWCUXc=oc, C Lo.VOZpgo obto~to.D _ FF pi f-)I -a(Z 05 4y ,~ 1u C1 4L hd It01o Note: All samples were analyzed inexcess of EPA recomnmended holding time (5I minutes) unless otherwise noted.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) % RS MV /TV X 100 number (mg/L) (mg/L) (acceptable range - 90 to 110%)
S2U0.50 a s l 01 Reviewed by Date reviewed
- LM.
Testing Solutions, Inc.
Page 6t Page L of I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, hlDL= 0.1 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst -a 7 Iodide reagent: l I C 1 Date analyzed O toC&Oi s Acidreagent: I wa vSk. I7 Calibration:
F. . l 't- ki.2*t. 0.10 mz/L I II.00m~JL Reference standard number l C )IltS2 l Z1 0 I Note: For samples with a residual chlorine of > 1.0 ing1L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control staufdard:
Reference standard True value (TV) Measured value (NIV) % RS - INIV / TV x 100.
number (mgfL) . (m&/L) (acceptable range - 90 to 110%)
WIGiS - .1* 0.50 o.'1T1 Duplicatesample precision:
Sample number
- IE4\Sample ID
______J__Duplicate
<C Sample characteristics S________ C__oUbl i
t o &
Residual chlorine S*J O .
Dko (mOtL)
.S0 I
%RPD - ((S - D)/[(S+D)flhj x 100 (alctptable range - 10%)
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number ._(mL)
____, ___, , Blank (shouldbe-<0.l0mglL) x -l.-;iOi.t C o<. oq mCs',Gc.42 -lC Cto "Lek C0.021 cS. OA- .Sb. 101 c .Ot A & <'( OO* 6'73 o~b.7-I ; 'I' Ii i*1 CnfiLot eAtr1C.L5 cJA( *co.ocoZOS OS0608. a& _ i A5twl (ol ?~eA -- -PAPc.r I eS c. U 5S llsam wereanal IEP yi s com en gi utsuness nootdzS Orwise N~ote: All samples were analyzed in excess of EPA recommended holding timne (15 mrinutes) unless otherwise noted.
I Reviewed by r-4k I Date reviewed I 0Z.09-S 1
-4qtJ,.
Page 5 1 Page I of 3 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst l bth,_ Iodide reagent: IIJ Rl 6 q Date analyzed lDtbo-II.cS Acid reagent: I Fipii5b Calibration:
. . .. - . -I 0.10 mglL I 1.O0 mglL -
Reference standard number IrI'S
&0 - I I SSR) I Note: For samples with a residual chlorine of > 1.0 mngfL, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Duplicatesampte precision: t Sample Sample TD Sample characteristics Residual chlorine %RPD - {(S; D) /I(S+D)1211 x 100 number fdd1 (mg/l) (acceptable range - : 10%)
P $bPnci 1IvIllV.s S o.0o03
-Duplicate 0. ., . D 4- L)e . -e Sam ple measurements:
Sample Sample [D Sample characteristics Residual chlorine number (mgiL)
Blank (shouldbe=<0.10Lm)
.. * .. *.. *2.*.*?... ,- Q
___l___ C'.ao S- 0ii d Ie. (0 L. sfOt bS411. 19 ge wlff C41 Y-.R ioeto I.
05D 1II. Ib zV, tui wi-P ,-V.0 C2w
_ _ _ . __ Lh n ( At A1
- P -O0 N5Dllale were aesLadc holin ndedtm( e n otherwise not.Z tsoW IJ. ObL Mr6it, W WVi h eonx 81 00A , 0.000) 06 Ntote. All samples were analyzed in excess of EPA rccormcendcd holding time (15 hiinutes) unless otherwiscenoted.
Reviewed by IKqA2 Date reviewed I ,t .4 05 .-
Environmental Testing Solutions, Inc.
A1 Page 52__
Page S of 3 Total Residual Chlorine
- (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst IV_ I Iodide reagent: I N-. I
_ f. l l
Datt analyzed I Dto-lt105- I Acid reagent: I N I Calibration:
I - 0.10 mg/L I 1.00 mg/L Reference standard number
- I _
Note: For samples with a residual chlorine of > 1.0 mglL. the calibration range must 6e adjusted to bracket the chlorine levels of the samples.
Laboratorycontrolstandard:
Reference standard True value (TV) hMeasured value (NMV) oRS - MIV/TV XIO0 number (mglL) (mg/I) (acceptable range - 90 to I 10%)
WsS xi 0.50 o.o qi4q qZ.el Duplicatesample precision:
Sample Sa~mple ID Sample characteristics Residual chlorine %RPD - ((S - D) /I(S+D)Y211 x 100 number ______ (acceptable range -*10%)
>,. ,Del;fe, MPrnotoiL, sU",4-ss . :S. .0.-;0;; 5"
-iDuplicate . .r: D 4o.cOo26 4 _
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number . (meJL)
- .** Bnk-.. (should be-< 0.10 mg/L) ,
b7 Iso4'. oQ ^t~-I fi U . (ic) a , C10 .C 93 bSole'. JS IIICoiML f e Z o.OI
'05soi)J.I ltn -tg(4L, W)l T YP lt __.___ (oq __,0 5 L.Ol.16 '
toL _ _ _ __L__ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
bl5bb lo .o, ' Il lCr . f- __ 0 6SoAIl.lO wend inexesolP re ng tme min s o.eooiso Note: All samples were analyzed in excess atEPA recommended holding timc (15 minutes) unless othenwise noted.
Reviewed by l K Date reviewed r Y-l.OS1
Environmental Testing Solutions, Inc.
Page 53 Page 3 of Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mglL Meter Accumet Model AR25 pH/Ion Meter Analyst I V Iodide reagent: lI. -- i Date analyzed [ 0 -11 K I Acid reagent: I Calibration:
- ~- _ 0.10DmeI L I 1.00 mgL Reference standard number Lo Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range niust be adjusted to bracket the chlorine levels of the samiples.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (rng1L) (mg1L) (acceptable nange - 90 to 110%)
. IWSS q-ID 0.50 aT q h4t79.
Du licatesampleprecision:
Sample SampIleD Sample characteristics Residual chlorine %RPD = ((S D) /[(S+D)12J1 x 100 number _ _ __1_ (mgfL) (acceptable ranee -*10%)
21 hS .j;ll. . .;
. -Duplicate .. D qq-Sam ple measurements:
Sample Sample ID Sample characteristics Residual chlorine number (me/L)
-. . K 'Blank (shouldbe =< 0.IOmgL) 6!Z~tOI0 VA 64 0 10% & 1iB> 40 Z-00 2q bZto1o.O3 _ INT n tC 1 en- 4..or t.Xo
. smolso _Ft6Ti P 00i C.,- .oCooqoI7 Note: All samples were analyzed inexcess or EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory controlstandard:
Reference standard True value (TV) Measured value (MlV) % RS bMV I TV s 100 number (mg/L) (mg/L) (acceptable rang - 90 to 110%)
lSsut 0.50 bq6O Reviewed by ktt Date reviewed r F 1.II -.05 i I
Page 10 Environmental Testing Soluftions. Inc. PagejI of I Alkalinity (EPA Myethod 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst IlI77777 Date analyzed ; Titrate samples to pH = 4.50 S.U.
Titrant normality and multiplierdetermination:
pH of Normality Normality (N) of HSOl pH Factor or Multiplier Deionized Titrant check Begin End Total - (5 ml Na1COj x O.05)/E = (V 50000)/ 100 ml sample water reference standard ml ml ml = 0.25/E -Nx 500
- 4.5 S.U. number . number (E) (acceptable range - 0.0I8 -0.0'2)
- 4. 1L INR l Jg1915 C3 (C11.9 21 0 0J5l Laboratory control standard:
Reference True value Sample Alkalinity(MV) % RSMI'lV/TVx1OD standard number (TV) volume Begin End Total Multiplier (mg CaCOIL) (acceptable range (Mg CaCO3IL) (ml) ml ml ml -90 to 110%)
Ns5ze o100 0o0 23.3 32iq- D.q ca 1 9w Duplicate sample precision:
Sample Alkalinity %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) J(S - D)I((S+DY211 x 100 number (ml) ml ml ml (acceptable range - 10%)
?aO51 fXBdr.4--6 - s 3 10 .6 s ,- F.e'D
. Duplicate 10o 0 }2
. 1; 5 D -
Afatrix spike recove Reference Spike value Sample Spike alkalinity (A) lstandard number (SIV) volume Begin End lTotal Miultiplier l(mg CaCOVL1) ll(mg Ca OV/L) l(ml) lml lml lmll WiS96 6l CO 0.0 ZOZlzo.2 1.5 l 21Q Sample alkalinity (By l Nleasured spike value (MV) %R MV / SV x 100 M
(mg CaCO..) NIV - A - B (acceptable ranZe (mg CaCOViL) - 75 to 125%)
il*(-*IS.....
00 ED Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (m? CaCO/L) oCqL-r W - SSLO A JQOL. 2b.T cO 30 _ _ Z-Vxstj4-5A m V6 oo I l 6 Lo l _ _ _ b i i 341~ .*6 - _________{° Reviewed by: Il Date Dtre reviewed: l. °
Page.- V)
Environmental Testin2 Solitions. Inc. Page I_ .oftC I Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed O Titrate samples to pH = 4.50 S.U.
Titrant normalityand multiplier determination:
pH or Normaliq y Normality (A) of HtSO4& pH Factor or h ultiplier Deionized Titrant check Begin End Total - (5 ml INa:COa x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml - 0.2'IE Nx 500
=4.5 S.U. number number __ (E) (acceptable range - 0.018 - 0.022)
___q _ IiJ IZ ioofjg4 oo 1.o 17o O. 020( io4 611L0.O- 0.0 =.0.ornI Laboratorycontrolstandard:
Reference True value Sample Alkalinity (MV) % RS - MV / TV x lO0 standard number (TV) volume Begin End Total Multiplier (mg CaCOj/L) (acceptable range (mgC3COL (l)(m) lml ml . -90to 110%)
1AS95 10 1oo0I12.U Z2.7 9.4 Io&.q /01 /00 28 Duplicatesample precision:
Sample Alkalinity %RPD D Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) ((S - D) I((S+D)ry1l s 100 number (ml) ml ml ml (acceptable range - I 0%)
.o;-o5- 6E.otsjp) 100 ZI.' 2q2 .5.S 31 ;.
. Duplicatc j atn- - 31 D £ 3-Z%
Aratr& spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume I Begin End Total Multiplier (mg CaCO/L)
(me CaCOL (ml) ml 4 ml ml INGsse 61) j00 2q.9-S32.4j eb.O lo. q 03 Sample alkalinity (B) Measured spike value (VIM % R - hlV / SV x 100.
l (mg CaCOj/L) hV - A -B . (acccptable range I (mg CaCOIL) 75 to 125%)
Z3 6 61 . 4 90 Samolemeasurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO;L oioBeS A rni W5 HLla 10 . 5~g3 O.k 35 3 '.3 C.o (1p43 &Z.
_ to _ _ I I T_ 0.0 6- .l 65.T, T_ I hH 1j 6 16.? 1 1 II S ISSW 14.0 1I I3.l I ___OS ___ __ __ I - ~I .5 1 3.1- 1 33 Oto.I-CO A rnaRS 14zo I0 -I 5 a1 23.5 iq.s & loI C. _ _ ______ '3o.V 3G.5ST .91 _ (if Reviewed by: I d1 I Date reviewed: I O.20-0o5 o l I
Page 20 Environmental Testine Solutions. Inc. Page 2_ of f i Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3 IL Analyst Ifo l Date analyzed Lii2Zi /q Titrate samples to pH = 4.50 S.U.
-Thxantxprmaliy and multip her determination:
pH Of NrmRNormality (N) of H1 SO 4 pH Factor or Multiplier Delonized Titrant check Begin End Total ( E (IVx 50000)1 100 nil sample water reference standard ml ml ml 0.2S/E
- 45 S.U. number number (E) (acceptable range - 0.018- 0.023)
Labratrycontrolstandard:
Reference True value Sample Alkalinity (MV) % RS = NIV / TV x 100 standard number (TV) volume Begin End Total IMultiplier (mg CaCOJIL) (acceptable range (mg CaCOJL) (ml) ml ml ml
- 90 to 110%)
Ct-j6 2550 100 100 ' q q4
.7. lo- /OI 16189 Duplicate sample precision:'
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) ((S - D)nl(S+D)y211 x 100 number (ml) ml ml ml (acceptable rane -e *10%)
____bt0o4 Alc)a oa< 100 0o0 9.0 q0 ; .q s Duplicate $ q0 _ /Oy io D2 .I'q.
Afatrix spike recoverv:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin 'End Total Multiplier (mgCaCOj/L) l (mg CaCOV/L) (ml) ml ml ml aIkali9ni nitl ityl qn 14°l 1I lr Sample alkaliity(B) Measured spike value (MV) % R NIV /SV x100 l (mg C:COA/L) NIV - A - B (acceptable range I (mg CaCO,/L) -75 to 125%)
q Jo 1 Sample measurements:
Sample volume Begin End Total Alkalinity.
Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOL)
O5cis:.Io l I ol - 0i3 100 l 3.o0 3Oq ?A l In. 790 I l 0 OR _oK.
OS0to I8. ll _ 31-S l -l 0 5. I34A l42. 2-8 _ -
OSo t.° 4 Z7- . __ 454 ___ __________
I _I___PPac_ _ I__ , 1J.? 16./B 6. LI _ - 1 oq A1..8 V ii 2tO Reviewed by: Date t reviewved:
rev I of, -tOOS1
Page 21 Environmental Testing Solitions. Inc. Page _g of II Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst l77G C]
Date analyzed l 7> -q 5§ l Titrate samples to pH = 4.50 S.U.
Tr..~ormalityand multiplier determination:
p of iH v Normality (N) of HISO pH Factor or Multiplier Deionized Titrant check egil otal (5 ml Na COj x 0.05)/E - (Nx 50000)1 100 ml sample water reference standard ml ml ml -rN 500 4.5 S.U. number number (E) (acceptable range - 0.018 - 0 .02 Laboratorycontrol standard-Reference True value Sample Alkalinity (NIV) % RS - INlV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOj1L) (acceptable range (mg CaCOjlL) (ml) ml ml ml -90 to I 10%)
if s 2 5 100 100 0.3 q2 1(. / If Duplicate sample precision: **
Sample Alkalinity %RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /R(S+Dy21 x 100 number (ml) ml ml ml (accepuble range -*h 10%)
______ 3 b 336.S 1 -2)10 S 110
/
Duplicate &) 06uI'i. .Z1 D l_
Mlatrit spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number l (SV) lvolume Begin End Total Multiplier (mCaCOvL
- (mg CaCOL) (ml) ml ml mll
/A0 5Z) 77'iq6 /10 vO Z bh i 210 Sample alkalinity (B) l R i=V / SV x 100 S(meg CaCOj/L)
I Measured spike value (MV)
MIV - A - B (mg CaCOV1L) l (acceptable range 75 to 125%)
I 110l lobD . o9?
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number . Sample ID (ml) ml ml ml Multiplier (me CaCOIL) oSot0.o7.0 l)XY /1(x) 19.0 lZo ,0 q 94 31 OSO Ot.o l I 2 / .22,1 25;1 j )l 31 105GO 1I.- 3 T, I0 TZ54_12_2_ 2 T _ _ I cso'a0o7.otlI-< 6I Q IjiJ 12 8 O L 1 ____M 9 ososotl T U.o2. 1U.. 1--
W. 1 Th - -(
Ll 1os1o3. -' A, F1 -. - 1 9 lOstcN i.O I I0 , I2.51 31 I - 36 Iav-7 L 1- 1I4.1 1f I 151 CYS0o,01.o01 3 1 . 14 172ZiD -we19 II/ 6 Reviewed by: __ _ _ _ _ _ Date reviewed: lot Uo I
Page ZZt.
Environmental Testing Solutions, Inc. Page 4 orfz_
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L Date analyzed l Ots 14N55 Titrate samples to pH = 4.50 S.U.
Titrantnormality and multiplerdetermination:
PIQ _Normality Normality (N) of lIaSQ, pH Factor or Mtultiplier Deionized 7r-nt -- cck_ Begin End Total = aml iaJs00)E = (N x 50000 t00 ml smple watcr reference shnodaird imt- ml 0.2SIE v Nx500
=4.5 S.U. number number (E) e.0OIS - 0.0212)
Laboratorycontrolstandard:
Reference True value Sample Alkalinity (MV) % RS - N1V/TV I OO standard number (TV) volume Begin End Total Multiplier (mg CaCO9JL) (2cceptable range (mg CaCOj/L) (ml) ml ml ml _ 90 to 110%)
Is p sp 100 'oo .2,0 J3,LS 16 Y /b0 16 ?,
Duplicatesam le precision:
Sample Alkalinity %RPD -
Sample , ample ID volume Begin End Total MIultiplier (ng CaCO,/L) l(S-D)/I(S+lD)P7Ij s 100 number . - (ml) ml ml ml . (acceptable range - 10%)
O~ 0 Co c' (j i 0 ' Lq2,3 /(j 10'{ S II: _______ ._
Duplicate J 0° IV. 1Q.L D -/I AMatriv spike recovery:
Reference Spike value mple l Spike alkalinity (A) standard number (SV) volume l Begin End Total Multiplier (mg CaCO,/L)
(mg CaCOIL) I(ml) ml ml ml IASS SR2) 6D 0 JQO 6- 1 5°I.3 15. 3 )JOq I/410 1, &
Sample alkalinity (B) Measured spikevalue (t V) % R - V I/SV x 100 l (mg CaCO,/L) MNIV - A- B (acceptable ran.e I . (mg CaCO3/L) -75 to 125%) I
.7)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample nutmber Sample ID (ml) ml Multiplier (mv CaCOVL) I ossb.cORz&& -rvh 1 6 l . 6.i 5 2) 1/1P OSot0 tO.O'-q I_ I 41.
0 ty -YSC_ _ _ 5"' 31.0 5d,14~2.
Osct. Io -O I 10 3S.
1a6lS.1 << 41 "S-qO 05o.10.05 ' rA 6Qtf1&.O3Ox _ 10-iq .1- 14 qo g/bo c.QlXlCA ;2 1 1 oo 0°V T4L lQu - - ______ I
_1q 1. - - -
Osopbo.07- TA 6~) l & ~T ;1ht1b0 (O. _
-rV60A It's 0(, rj to. W16) I 0Id.11qS.917 1 1 ItI Reviewed by: Date reviewed: IO mr2o-Q Dteiw:::- 1
Page 2 3 Environmental Testine Solutions. Inc. Page __ f f Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst M )%,
Date analyzed l Titrate samples to pH = 4.50 S.U.
Titrant norality and multiplier dt ination:
pHr NOMM - ~ _ Normality (N) orH2 SO, pH Factor or Multiplier Deionized Titrant check Begin nr- S_ C0 X 0.0;)/E = (NI 50000)/100 ml sample water reference standard ml ml ml =0.25
-45 S.U. number number (_E) (acceptable ranage - 0.018 - 0.022)
Laboratorycontrolstandard:
Reference True value Sample . Alkalinity (NMV) % RS =MV I TV x 100 standard number (TV) volume Begin End Total iMultiplier (mg CaCO3/L) (acceptable range (Mg C2CO3L) (mI) ml ml ml -90 to I110%)
IAcSPS 100 100 7-q 37.fo II 0.C) / 1/ 1 Duplicate sam le precision:__ _ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) ((S - D)Ii(S)Dy12il s 100 number (ml) ml ml ml . (acceptable range - 10%)
__3___ ASON 12 j /O.') s37.L 40 :; Q.L D oo-4z Duplicate 4O- 3 3 _ D _ i,_37, Mfatri&spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CsCO3IL)
(mg CaCO3L}) (ml) ml ml ml ti -49625 ER) 100 1.. 1o l2.i 0-0 1 O.'J 2.I Sample alkalinity (B) Measured spike value (NIV) % R - MV I SV x 100 I (mg CaCOVlL) l MV - A - B I (acceptable range I F (mg CaCOVL) 75 to 125%)
4q Sample Jneasurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (mI) ml ml ml Multiplier (me CaCO3IL)
OlSOblO.a 0 IA Ql/ (o 12.0 l/.0l lo. C
_______I ?O C I 5? ___ IOA0 l/ JCS,20 I 8.7S 2.OSc I Sk 1A Il I q. 30.3 656 :o O9o +/-2JiL. CAM zct.I '2.0 b.q IC?3 120 l l CAe l l Reviewed by: I Date Dlte reviewed:
re e l OL 2,1 t5
Pago 1:.
Environmental Testing Solutions, Inc. Page L of I Total Hardness (EPA Method 130.2)
Matrix: Water, MDL- 1.0 mg CaCO 3 /L Analyst l l lII]
Date analyzed Titrantnormality andmutplier determination:
Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2E = (V 50000)/ 50 ml sample number number _E) (acceptable range -0.0 IS -0.022) -Ns 1000 WI£RZ1 _9ijZjs_ DO 'q ? - 0.b -2_.
Laboratorycontrolstandard:
Reference True value Sample Hardness (MV) % RS = MV I TV x 100 standard number (TV) volume Begin End Total Mlultiplier (mg CaCOAIL) (acceptable range I52' mgCsCOt) J lJ ml ml ml _ 90 to ItO%)
ip -A 40- 50 Ct-4 Ilu~o J.9 -2 0('O 39 9 CP 50)
Duplicatesample precision - -
Sample Hardness %RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L) f(S - D)II(S+DY21) s 100 number (ml) ml ml ml _ (acceptable range - a 10%)
5U j1.@ LO
.t j SSLO Duplicate
-t i.'4 1
.* Zo s_ jo D
D S4$3 L
Matrix spike recovery:
I Reference standard number s a lSpike value lSample l I(mg I'
(SV)p OLCOV) 4 LR volume Begin End Total I(ml) Iml Iml ImlI 1 55 1na j i.0 II.
M1ultiplier Spike hardness (A)
S (mg CaCd A)
Sample hardness (B) Measured spike value (NIV) % R -,MIV I SV x 100 (mg CaCOqjL) NMV - A - B . (acceptable range ll (mg CaCOj,/L) _75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg Caco()
l - l Blank l (shouldbe 0 mgCaCO,/L) l Sb 0.l 0.0 0.0 ZO.( T _ _ _
l 02 jC75 A DocWT6 ,
fn h5 V12_
~ _
G.-+
R {Z-9A~
URo Vs6 1 .l 4___0 fl.Q 3JiLJ L *° ____4._ ___
Note: If >I5ml of titrmnt is used, sample must be diluted. Reviewed [ r Date reviewed I o' O W.6t5 I
Page lt Environmental Testing Solutions. Inc. Pagel of 5 Total Hardness t4 p t e Z_
(EPA Method 130.2) CA) l O Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst l,@- I ;nt'?/
Date analyzed ID, 14 _UJ5 Titrant normality and multiple dtriation:
p deermi Titrant Normnality cbeck Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E (IVx 50000)1 50 ml sample number number (E) (acceptable range - O.01s - 0.022) =Nx 1000 11J Rtc , sR 3 O.O l O0 1 . .0tO ' t Laboratorycontrol standard:_
Reference True value Sample Hardness (MV) % RS - NIV / TVx 100 standard number (TV) volume Begin End Total Mfultiplier (mg C2COj/L) (acceptable range (mg CaCOO/L) (ml) ml I ml ml - 90 to 110%)
155 AA7 40 50 ID0. O 12 Z -27.Z I*ZO
- 44 u10o-o7. I 4 .*.
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml lMultiplier (mg CaCO/L)
I /; t) 1 _ (sbould be - 0 mg CaCOIJL) D) - 0-f O- 2) 0 ND OS06ta.c17z SOO la__ U.?. . 'qt 4.4 -S I so(Z 0,.QZ. Sa m (o) Z._. 3q.-2 4j.o 8 GO(OC 0(.. 02. Sa ba Is c~ 0, a ,z..
80__ _lq t VA1 ea_ __ _
o blO . G;3 ,G4F 6o t O5WSZ3'o ,6e S@ _& ; 4 ;z.4Q>
.. 3.A = _ _ _ _ _ _ _ _
OS 0 b 10. O3 $ rW esmX£.(h ts 42AL 38 s.9 it bes t0. 0 2A 5 .fiO' 1. t 6 OSOC.Ot.2& PAIP CooQ A5.. 34- 1Z. () 45Z=
Os0oLOL. O.C 4 PAtr 00% __ _IO 0 . 1o-. 4215 520 05<t66L04P .. % (3p itm 0.0 3- . ISO5
)-15a Note' If > I SMl of titnrnt is used, sample must be diluted. Reviewed by :
l]
I L ( Y .J-Date reviewed E31O S
Page Iq Environmental Testing Solutions, Inc. Page 7- of 5-Total Hardness (EPA Method 130.2)
Matrix: Water, IDL = 1.0 mg CaCO3 /L Analyst Date analyzed [I i I5 II]
Titrant normality and multiplier determination:
Titrant Normality check Begin End Total Normality (N1of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E (Na 50000)/50 ml sample number number (E) (acceptable range 0.018-0.022) iNX 1000 Laboratorycontrolstandard:
Reference True value Sample Hardness (MV) % RS - MV / TV x100 standard number (TV) volume Begin End Total Multiplier (mg CaCOAL) (acceptable range (mg CaCO/L) (ml) ml ml- ml _ 90 tol 10%)
IV=S ZZ' 40 50 vt. 23i 2.o 200 IC) Ih7Ol Duplicatesam Ic precision:
Sample Hardness %RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) ((S - D)/[(S+DY2[) x 100 number (ml) ml ml ml (acceptable range-i 10/)
cfso - Z9 t rNr o a, 3 l.S nn
-i*1 2 StSL -Z sd f Duplicate l. S .1 D4 D
Matrix spike recovery:
Reference Spike value Sample Spike bardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO/L)
_(mg CaCOj/L) (ml) ml ml ml I oIS& 'ZZI 40. 50 -,"2l.Z_~ Zo 1,1 A t ll.
=7 14.
Sample hardness (B) Measured spike value (NM) % R -MV / SV x100 l (mg CaCOIL) MVV=A-B (acceptable range (mg CaCO,/L) -75 to 125%)
_ample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml nml ml Multiplier (mgCCOC3L)
(should be - 0 mg CaCO,/L) l _ _ _ _
t Po(4.OS X Ca) So 23.2. 3t.0 1. 0 1ICC. l1cIO olot.0t. 2.4 fl&,p ( 3180 sem -Il 1l160 1
&JVV" _____ - llI os.tolo 1\tMsw() .0
.'S l l l 1(*
dSX QAN7Z35L l`50b64.l 7- 9s _L
- I -IS VS -. I l-u l
azo(od.jO l CzT9 _ __ l zo.zT 4.2. z.o 1 1 ___ _ _ I 0sco(o1.
cosol.o 00e I 7btoe1m1wt4w T __2_._2_
_____ 2 Z Zo.3 ?_ 1.1 7.0 I 4 Note: i lml oftitrant is used, sample must be diluted. Reviewed l l Date resiewed I OC-?200S by: lI .
I
Page ZO Environnmental Testin2 Solutions. Inc. Page 3 o Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst Date analyzed li c4IIJ, -
Titrant normality and multiplier determination:
Titrant Normalify check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.21E (,Vx 50000)150 ml sample number number (E) (acceptable raoge-0.018-0.022) -Nx 1000 Laboratorycontrol standard:.
Reference True value Sample Hardness (MV) % RS - v1V /TV x 100 standard number (T) volume Begin End Total Multiplier (mg CaCOj/L) (acceptable range (mgCaCX) (ml) ml ml nl -90 to 110%)
1M& 2Z1 40 50 ?4 3 t. Ito Duplicatesample precision:
Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) I(S -D) 1[(S+DY2lj x 100 number (ml) ml ml ml (acceptable range -fi 10%)
_C____C_ .OL _-__6_
_ _ sc5 'v.3 ao 2 .t zO S;_ _
Duplicate e ' Z 3 1 ,C4 Z24 4 D 5A CaC3.e70 Afatrix spike recovery:
Reference Spike value Sample. Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mgC&COi/L) l(mg C&CO31L) (ml) ml ml lmll
\IiSs 12N- SO Z--3 72c M-S 41 20) I8 Sample hardness (B) Measured spike value (MV) % R - MVISV x 100 (mgCaCO/L) MV A- B l (acceptable range (mg CaCO/L) -75 to 125%)
5; . 'qS.O7o Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) nml lml ml Multiplier (mg CaCO3L)
Blanka )- -
ICvStb@1 ,iL lE;J; eiwbnb 0 50 35-3 lts* il .A ZOX I
lOBc at C D) 50 1 38.1 3.o l G
°S~t- 16°alt b ZU 5ox.0 38.1 40.-7 A.O 40 bSttwo I aaI ALs 5° 01 qo 43.B G NO 50btoISI-bI AlAA 002. l 50 0 3-1 3.1 l _
0-xpI-. 0 AU-O 01?, ___ ___
rt is. _' A0 QD _ __ .e 1.qI q.j
- _ II ocoC1e-o2 ALCOA, j 5 i° 170a j Ita.) lb 0! oso&S.O'I ALtA 01 (3_O 5_ l 17.0 IMll .l l Note
- If >1 SmI of titrant is used, sample must be diluted. Reviewed Date reviewed I 20- OS by: 14 I
Page P_ _
Environmental Testing Solutions, Inc. Page Lt 0f 15 Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst FIM II Date analyzed [ 4-0S l itrantnormality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Mlultiplier reference standard ml ml ml - 0.21E (N x 50000)/ 50 ml sample number number (E) (acceptable range-0.018 0.022) -Nx 1000 Laboratorv controlstandard:
Reference True value Sample Hardness (NIV) % RS - NIV I Tv s 100 standard number (TV) volume Begin End Total NiMultiplier (mg CaCOjIL) (acceptable range (mg CiCOjIL) (ml) ml ml ml - 90 to 110%)
XWaSS aX7 40 50 X5t;.; 2.l R2 4- 10°5 Duplicatesam le precision:
Sample Hardness %RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D)I(S+DY21) x 100 number (ml) ml ml ml (acceptable range 10%)
Q ) 50W10 O Jc2/, d?3.5 2.3 ;0 s 46 g ~ Y e Duplicate _ SO .35;. l ao Df Matrix spike recovey:
Reference Spike value -Sample Spike hardness (A) standard number l (SV) lvolume lBegin lEnd lTotal l Nultiplier l(mg CaCOs1L)I I (mg CaCOiIL) l(m ml ml ml IfISS ;t'7 qo 50 l23.l7C-. a0 l l t(
qO .
Sample hardness (B) Measured spike value (MV) % R - MV / SV x 100 l (mg CaCOIL) MV - A - B (acceptable range I I (mg CeCOVL) -75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml nml Multiplier (mg CaCO/L)
Blank -m C - _ _ _
g l ~~(should be - 0mgCaCOL) lI l l I-lA Al._-_______ 50 17.to 31.l 2 - 0 icAQ- bXtik 60 ZI.sS 35.9 llI 8LYO PPAk -i.Q A, 50 35.B q0.j1 _ l___
_______ p.oe-o 6S OS 1 4.s . 4
_MS-A ov~oe-s I O 140- 4 X.1 4 13
_ K__ tMi- GnooS -o50 l 666- 4.0 /.0 80 lA___ l4 o_ 0lo l 50 4.0 8 4.', l_ l 9_
_ _ Mis-6 00,'i 50 G.4 1l -7 I1 _ _____
dlsq tiN ao.f6X1 o Ia.7 15. v.1 -? . 4, i11L I. I_______
I I. .
I I - v - A Note:If >lSmloftitrantisusedsamplemustbediluted. Reviewed Date revee l a-1&0 by: j -]l
Page 22 rnvirnnmPntal Testing Solutions, Inc. Page 5 of S Total Hardness (EPA Method 130.2)
Matrix: Water, MDL 1.0 mg CaCO 3IL Analyst .
Date analyzed 06-lq14 5 Titrantnormality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E (V x 50000)1 50 ml sample number number _ IE) (acceptable range - 0.018 - 0.022) = X x 1000 Laboratorycontrolstandard:
Reference True value Sample Hardness (NiMV) % RS - A=V / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable range (mg CaCO/L) (ml) ml ml ml -90 to 110%)
xZvss dA 7 40 50 a1. 7 2q 2 *-3 0 46 ./
go4.O 0 50 aCO 411 O o Duplicatesam ple precision:
Sample Hardness %RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) ((S - D)1I(S+DY)21J 100 number (ml) ml ml ml (acceptable range 10%)
ii:c- *1b-Q~ -O 150. e 17.3 d.S3 R0 s Duplicate 50 17. 3 5 ;L el D Mdtrix spike recovery:
Reference Spike value Sample l Spike hardness (A) lstandard number l (SV) lvolume lBegin l,;d lTotal l Aultiplier l(mg CaCO31L)l (mg CCOAc)tL (ml) t5)ml3m*lm IJ &Tmi
.:1S -;855 7 40 50 c1.:d41 dD l Sample hardness (B) Measured spike value (MV) lVISVx 100H n (mg C&COjL) hlV-A I - BI (aceeptable range (mg CaCO31L) /@ l -75 to 125%) l Sample measurements:
ISample volume lBegin lEnd lTotal l Hardness Sample number Sample ID l (m) ml ml mll Multiplier (mg CaCOIL)
I _I ~(should be 0 mgCaCOVL)l I. I l li1;-Aoto-i6-CS 50 a.o(P.O. 30 1 4 l O l l w-EW sM-11. O 50 1 0.1 134-.1 4. I AO OafS t1% &C. OQY1- _S _60
_34.a U.4 A 1I_a__ _ao
, Lek I 1 IC 2-1b 1-. 1501 15. 1 QZ I- I_ I Note: If >1 5ml oftitrmnt is used, sample must be diluted. Reviewed l1 ' Dat reviewed [
(j 6- 20-S I
Sequoyah Nuclear Plant Biomonitoring June 7-14, 2005 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.0 '
I I
I I I, I. I - .- .-
rI I I . I i I I I I 0.9 State and USEPA Mandated ControlLimits Limits Set According to +/-2 StandardDeviations 0.8 0.7 0.6 U 0.5
- 0.4
.I
' I I I I I I I I I I I I I I I I I I I I c9 1.0 l I I I I Il 1 1I II I I I I a I 0.9 USEPA Warning and ControlLimits Limits Set According to 10 h and 25h Percentile CVs 0.8 0.7 0.6 _ .......... ......... ---
0.5 0.4
~~~II I ,I I . . I I I I I I jr4T ¶I. SVdat et% '5 %'
Test dateI 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- - Central Tendency (mean IC25 )
Warning Limits (mean IC25 SA1)
. Control Limits (mean IC25 4 SA25 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water State and USEPA Laberataty. Lb.oratery USEPA USEPA Telt sumber Test daft 7-day ICS, CT S Control Lmits S.lo Warning Units SA.S Coetro! Limits Skis Wandtng Umts S. central C Lnits Cv (dLKCI) WLCKCD CT.2S CT+2S CT-SA. CT+Sr+f Cr.S,. Cr+S,,, CT-SkSi CT+SA.19 CT-SA. CT+SA."
1 10-19-04 0.52 2 11-02-04 0.68 0.60 0.12 036 0.84 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.20 3 11-09-04 0.54 0.58 0.09 0.40 0.76 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.16 4 11-30-04 0.54 0.37 0.08
- 0.42
- 0.72 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35- 0.79 0.26 0.31 0.83 0.13 5 12-07-04 0.64 0.58 0.07 0.44 0.73 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.Q1 0.26 0.32 0.85 0.13 6 01-11-05 0.63 0.59 0.07 0.45 0.73 0.07 052 0.66 0.12 0.47 0.71 0.22 0.37 0.82 0.27 0.32 0.86 0.11 7 02-084-5 0.62 0.59 0.06 0.47 072 0.07 0.52 0.67 0.12 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.11 S 02-12-O5 0.62 0.60 0.06 0.48 0.72 0.07 053 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.87 0.10 9 03-01.05 0.61 0.60 0.06 0.49 0.71 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 t0 03-01-05 0.61 0.60 0.05 0.50 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.Q3 0.27 0.33 0.87 0.09 11 03-08-05 0.69 0.61 0.06 0.50 0.72 0.07 0.54 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.27 0.33 0.88 0.09 12 03-15-05 0.62 0.61 0.05 0.50 0.72 0.07 0.54 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.27 0.34 0.88 0.09 13 03.29-05 0.67 0.61 0.05 0.51 0.72 0.07 0.54 *0.69 0.13 0.48 0.74 0.23 0.38 0.85 0.28 0.34 0.89 0.09 14 04-12-05 0.61 0.61 0.05 051 072 0.07 0.54 0.69 0.13 0.4S 0.74 0.23' 0.38 0.85 0.28 0.34 0.89 0.08 15 04-26-05 0352 0.61 0.06 0.50 0.72 0.07 0.53 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.27 0.33 0.88 0.09 16 04-26-05 0.58 0.61 0.05 030 0.71 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.38 0.84 0.27 0.33 0.88 0.09 17 0543-05 0.61 0.61 0.05 0.50 0.71 0.07 053 0.68 0.13 0.48 0.73 0.23 0.38 0.84 0.27 0.33 0.88 0.09 18 05-10-05 058 0.60 0.05 0.50 0.71 0.07 053 0.68 0.13 0.48 0.73 0.23 0.37 0.83 0.27 0.33 0.88 0.08 19 05-17-05 0Q50 0.60 0.05 0.49 0.71 0.07 053 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 20 06-070-5 0.62 0.60 0.05 0.49 0.71 0.07 053 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 Nble: 7-d IC, day25% hkins eoncentration. An estnoatbn orthe concentntin ofpotassim cheide that woud cause n25% reduct'in hi Pimephis owtb for the teat popution.
CT- Cratnal tendency (moamlC2 ,).
S - Standard devWtmn ofthe IC,, wa)es.
Laboeatory Centrol ant Wanrng Limits Labotorycontrol and w ig mb ere atblshed using the standard dction of the IC vaes cemspondig to the 10th and 25th prcent& CV. These ranFes are more rtigent dhn the control and wan g lnk recommended by USEPA foe the teat method and cadpot.-
9AIj - Standard detion correaponding to the10' perventil CV. (SAI9 - 0.12)
SA2 - Standard deiition corresponding to the 25' perctile CV. (SA2s - 0.21)
USEPA Centrol and Warnrin Lbibt SAki- Standard deviation coruponding to the 75' pucentile CV. ( Sp-" - 0.38)
SA9 - Standard devation eeresponding to the 90' percente CV. (SA6 - 0.45)
CV - Coefclment of isoation ofthe IC-, values.
USEPAn2i0g hg miE rMetd toW- VurbftyktWhoL-Met ToxkkyAppkbti tWbduthe NtbarobantDkcrbe Emkatb& Fro. tAll-R-,US Snvt IErA cbtaltot-tin AYey.. Chkrnttt OH.
O, n... qurl S yf . le0 IncIldj,_. 064705
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pitnephalespronielas using Moderately Hard Synthetic Watcr 1.0 I I I I I I . I 0.9 0.8 2 0.7
- 0.6 In q04 U
-' 0.5 0.4 F -___ - ----- -_-_-_-__-______- - - - - - - -- _______ -
0.3 -
0.2 1 1 1 . I I I I I I I I I I I I I I I I 1-1PS"NNA \3N'
.% O-."- %-1 PJO~ s e %-5-0 %Tst Odtes %N. o-X %0 op rj°5 Os" to Test date 7-day
- 1C25 = 25% inhibition concentration. An estimation of the concentration of potassium chlorde that would cause a 25% reduction in Pimephalesgrowth for the test population.
- - Central Tendency (mean IC25 )
Laboratory Warning Limits (mean IC2 5 + SA.10 iSA 10= 0.12)
.......... Control.Laboratory Limits (mean ICs+S *A25 A25= 0-21)
-- USEPA Warning Limits (mean IC25 + SA75, SA75= 0.38)
USEPA Control Limits (mean IC25 S90, A SA90= 0.45)
Environmental.Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Control Control M~ean Test date Snrvl Growth CT CV CT hlSD PMSD CT Survival Growth for Control Growth (%o) for Control (0/) (mgtlasvae) (mglarVi) (%) for PNISD (%o)
Nrowth CV (%)
1 10-19-04 100 0.757 1.1 0.09 12.3 2 11-02-04 100 0.756 0.757 10.9 6.0 0.15 19.3 15.8 3 11-09-04 100 0.609 0.707 7.8 6.6 0.11 18.0 16.5 4 11-30-04 100 0.689 0.703 15.7 8.9 0.10 15.1 16.2 5 12-07-04 97.5 0.687 0.699 7.6 8.6 0.09 12.7 15.5 6 01-11-05 100 0.797 0.716 8.7 8.6 0.10 13.0 15.1 7 02-08-05 97.5 0.687 0.712 3.9 8.0 0.11 15.5 15.1 8 02-12-05 100 0.712 0.712 5.8 7.7 0.11 16.0 15.2 9 03-01-05 100 0.749 0.716 11.8 8.1 0.17 22.7 16.1 10 03-01-05 100 0.889 0.733 4.5 7.8 0.12 13.7 15.8 11 03-08-05 100 0.735 0.733 8.3 7.8 0.08 11.0 15.4 12 03-15-05 100 0.533 0.717 3.1 7.4 0.06 10.5 15.0 13 03-29-05 100 0.676 0.713 12.5 7.8 0.12 17.4 15.2 14 04-12-05 100 0.702 0.713 9.8 8.0 0.10 13.9 15.1 15 04-26-05 100 0.742 0.715 3.9 7.7 0.09 12.3 14.9 16 04-26-05 97.5 0.702 0.714 7.2 7.7 0.10 13.5 14.8 17 05-03-05 100 0.760 0.716 2.5 7.4 0.10 12.9 14.7 18 05-10-05 100 0.772 0.720 10.7 7.5 0.10 13.5 14.6 19 05-17-05 100 0.858 0.727 6.0 7.5 0.06 7.4 14.3 20 06-07-05 100 0.848 0.733 3.3 7.3 0.09 10.1 14.0 Note: CV-ICoefficient of variation for control growth On aveage, the CV for control growth is 73% in Environmental Testing Solutions, Inc. Pimrephaler chronic toxicity tests.
Lower CV bound determined by USEPA (10I percentile) - 3.5%.
Upper CV bound determined by USEPA (90' percentile) .20%Y MSD - Mirnmum Signifkcnt Difference PMSD - Percent Mininnu Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent diffemence between the control and treatnent that cinbe declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 14.0% from the control Lower PMSD bound determined by USEPA (10' percentile) - 9.4/.
Upper PMSD bound determined by USEPA (90' percentile) - 35%.
CT - Central Tendancy (mean Control Crowth, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephalet growth in chronic reference toxicant tests USEPA 2000. UnderstaMding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.
EPA-S33-R-00-003. US Environmental Protection Agency, Cincirnnti OH.
Organfsms obtainedfrom AquaticBloSyserns. Inc. 0)6407-05
Environmental Testing Solutions, Inc.
PimephalespromelasControl Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 I I I I I I I I I I I I I I I I I I I I 1.25 1
-S
- C4 1.00 I o es
.. - ~...................................................-.......... ..... ,
0 Ia a
o.
.= 0.75
.o
=: _ _.._~ ~..._................_...._.. ................. _.._. ._.._.._.._.._.._..
U E 0.50 0.25 Minimum Acceptance Criteria (0.25 mg per surviving larvae)
I- I I I I 1 I I I I I I I I I I 40 North Carolina Acceptance Limit (< 40.0%)
S =
30 Kentucky Acceptance Limit (< 30.0%)
I -,
o.4 20
~ *----;....----.'.----.......................................................................................................
u Is CJL) o 10 0
U 0 E .............. I Test date
- Control Growth or Coefficient of Variation (CV)
- - Central Tendency (mean Control Growth or CV)
. Control Limits (mean Control Growth or CV + 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water I I I I I I I I I I I I I IIIII 40 30 20 10 ..................................................................................................................................... .....................
0 Test date
- PIMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- - Central Tendency (mean PMSD)
.Lower and Upper PlNlSD Bounds Lower PMSD Bound ( 1 0 h percentile) = 9.4%, Upper PMSD Bound (9 0 tb percentile) = 35%
(Lower and upper PMSD bounds were determincd by USEPA for the method and endpoint.)
QA Reference Toxicant Summary Laboratory name: Environmental Testing Solutions, Inc. .
Address: PO Box 7565 .
Asheville, NC 28802-7565 Contact person: Jim Sumner .
Test species: Pimephalespromelas .
Reference toxicant: Potassium Chloride (KCI)
Test type: Chronic Dilution series: 0, 300, 450, 600, 750,900 mg KCI/L Type of control water: Moderately hard synthetic water Reporting period: April - June, 2005 Test date Organism IC2 5 95% C.I. Average control age growth (mg/L) (mgJL) (mg/imitial larvae) 10-19-04 24-hours old 517.0 273.2 690.2 0.757 11-02-04 24-hours old 683.9 536.8 692.3 0.756 11-09-04 24-hours old 537.6 129.8 674.1 0.609 11-30-04 24-hours old 540.8 333.7 639.6 0.689 12-07-04 24-hours old 638.8 537.5 718.8 0.687 01-11-05 24-hours old 627.2 558.4 663.4 0.797 02-08-05 24-hours old 616.4 490.7 667.8 0.687 02-12-05 24-hours old 619.7 503.9 687.9 0.712 03-01-05 24-hours old 612.2 ,448.4 682.4 0.749 03-01-05 24-hours old '607.5 499.8 651.9 *0.889 03-08-05 24-hours old 689.2 606.8 807.1 0.735 03-15-05 24-hours old 619.0 520.6 686.7 0.533 03-29-05 24-hours old 667.7 311.7 796.5 0.676 04-12-05 24-hours old 614.2 453.8 683.3 0.702 04-26-05 24-hours old 523.6 406.7 610.8 0.742 04-26-05 24-hours old 579.9 99.4 655.4 0.702 05-03-05 24-hours old 610.1 563.3 637.9 0.760 05-10-05 24-hours old 580.6 513.3 658.0 0.772 05-17-05 24-hours old 504.0 418.4 615.9 0.858 06-07-05 24-hours old 618.4 529.7 651.4 0.848
Environmental Testint! Solutions. Inc. Pagel1 of '
Environmental Testin2 Solutions. Inc. Page 1 of5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pinephalesjpromelas PpKCICR Test Number: -tJ6 Dilutionpreparationinformation: Comments:
KCI CHM number: LA" ft8 Stock preparation: 50 g KCVU Dissolve 50 SKCI in I L Deionized water Dilution prep (mgfL) 300 450 600 750 900 Stock volume (ml) 6 9 12 15 IS Diluent volume (tnL) 994 991 9SS 985 982 Totl volum (mL) 1000 100 1000 . 1000 1000 Test organisminformation: Test information:
Organism age: TZ.vS Por4 03.I_ Randomizing template: fe4FP Date and times organisms atc-CAo-S VS rotS~ c Incubator number and 3 were born between:. ._shelf location:_
Organism source: A6% B$c~rJ) csa S Artemia lot number: 6 to0l T Transfer bowl information: pH = SU Temperature = C Total drying time: 1+
__S3__ Z4 1 C. Date / Time in: C,.-i4.cJ 2 Average transfer volume: t .. Q Date / Time out:6 iS-m- L5 Oven temperature: toOC Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, MNHS Analyst feeding feeding time renewal, or batch used time termination time 0 o6z51oS __ _ ___ _ XI CoQSA 4 1 b O I oo ______ . O3cdocMCe5A d 2 __-o__l 1555 1KS7:54 q-uSe MO .. A 3 0 -tS% s.tob %zsSk &tCcA-CUSCL 4 4 cia-tb-ds . Io'sS50 l3 ,.-C- Os 1\ j 5 ____il2.-c5 15soQZ 134AO N-C)c2*Os EK 6 oi 5-OS olso 8 l . I oL-Ot-'s jj 7 l l S .-:......;'.. .... 31 Control information: . Acceptance eriteria S Summary of testendpoints:
%Mortality: O20h 5 l 77-dayLC Average weight per initial larvae: a. EIR O ; :. . .<.- ;l NOEC l 5O Average weight per surviving larvae: Z.,0.25 g/larvac LOEC to ChV I C15 _ _ _ _ _ _ _ _ _ _
Environmental Testing Solutions. Inc. Page 2 of 5 Species: Pimephalespromelas PpKCICR Test Number. (ovo Survival and Growth Data Day 0
A rB Control C D E 300 mg KCIIL F G H I 450 mg KCVL J K L 1 10 10 10 /I0 /0/l 1/ /O/0 /0T0 M° l IC l 10) l I)CY I It-OlIC/)l/
3 IC t O /O1 O /0 /O /6 _ /) to) -/0 4 ~ IC)O
/0 I0 /) l/O O /to /OlIt 10O I 10O '16' l /0 I0 1Ib {/OlbI0IG S 10 10 10 10 10 10 to 10- lo lO 10 to
_ _/0 16 /06 j /0 /b / _ /_ 0 A- Pan wightmg_ _ __
AnlytACSl4<o1il tlul 87 lq.52 NA.!5 tNA3 I's-8lo lqg4sqIt,3l B - Pan + Larvae weight _
Aals: uj S.so 23,3Z 22.4t2 22.8i 23,1b 22 .Sl 2276 7b %bA ZZA .2. 91z.8ZZ13 22.34 Laaeweight(mg A- - _
8.tvi g.tA V.,S1 8-o1 S8.Wq -1.5a 1.83 t.-W 1.91 8.28 &.1o 7.S3 Weight per Initial number of larvae (mg) 0cK I 0 I& "@1 j N%,1
- C I Initial number of larvae 9" O1 O O Or .O *0 DI Average Percent weight per reduction Initial from control O. £'i R O.8Ri& a - 0-. - .5 number of (%)
arve (mg)
Comment codes: c = clear, d = dead, fg - fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.
Calculations and data reviewed:
Comments:
Environmental Testina Solutions. Inc. Page 3 of S Species: Pimephalespromelas PpKCICR Test Number: td o Survival and Growth Data Day 600 mg KCUL - 750 mg KCVL 900 m KCiL
. M N 0l P Q R S T U V W X O_ . 11 010 I 1 0lo ito /0 / 10 1 /0 0 /0 I0 II? 1____I_____ 1A
-- 2. 12z 'z& t 416 It) /0 /0 /O _ 8 2 2.Z 3D4 4_I_ /0 /t /D 14 8 1 (o 2- Z __
45 *rio/ ( /0 8 1
-I a 6__ _ _ _lo lo o o 4 1 btO _ _
6 ZI 'I) g9"i d -Ii i- j4g4 7 o 10 t8 9 4 __
A - Pan weight (rg) _
Tray color code:: I I'.S ti . 10 1q.it q14 l .OS 1q.9s 15.06 %9..O 1II.A 143, A nalyst: - E t a14 iv 5 N. s ol t4. IL .. S iq t B - Pan + Larvae weight (mg)'
Analyst: :I .-- Z Zl0 .21A 22e4 210 l1 . A 18i.23 OIq.'S 1q.9_1 Is2.5 1.z Larvae weight (mg) - A- B 5.'S 1.198 .q4 1.2. 2.25 3. L1W0 4.o0 _ -_ ,3A Weight per Initial number _ l _
of larvae (mg)O O- b o o o *o o O av O O
-C/ Initialnumber oflarvae Average Percent weight per reduction Initial from control O.oZ 2 0
.l1% O.3176 56:1% O.O0ZSq 1i.i~
number of (%)
larvae (mg) _
Comment codes: c = clear, d = dead, fg = fingus, k = killed, m = missing, sk sick, sm = unusually small, Ig unusually large, d&r - decanted and returned, w = wounded.
Calculations and data reviewed:
commtents:
Environmental Testing Solutions, Inc.
Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pmephalespromelas Quality Control Veriflcatlon of D2at Entry, Calculations, and Statistical Analyses Tnt amben PpflCICR 106 (A" o5351 Dtp SLI Taid.tess Je17-t142005 Rteywedbyi
(.we L'.*eM~(J Wftk41I1n- q 64. C-4Mkw d .6hsWftk.I 3"od.
- N66M".d P4-.Og
.666 .a. C~dwed.9 ?..I 5-.4.56.5
( -
_X (S4. Jew*e -)i~.I b_ 6-A.-ft. n B- f.L-
_0g4td - A.11 _Ae btud -6.
.M
- 1. W 1h O_" b ) ~ "Wd A 10 lo 3144 21.5o I66 0866 0366 C9,4 B 30 30 14.61 23.32 3.63 0.863 0.848 3.3 0863 fools *M8 3.3 Ne.tapplicable C 10 30 1438 22.92 8.34 0.8o4 0 8e4 D 10 30 1482 22.89 1.07 0.107 0807 .
a_ 10 to 14.52 23.16 *.64 0864 0664 30 r -0 30 14.93 22.51 7.38 0.75s outs 07 10o.o 88 "is 43 3.6 G 10 10 14.93 22.76 7.83 0.783 0.793 30 0 14.91 23 64 8 66 - 0166 0.666
. 10 Io0 3.30 2291 771 0.711 0.781 450 JO 30 t0 1454 2282 3.28 0 0.793 42 0 828 loo ..7s93 4.2 Ls K I0 10 146 22.73 8.10 H"! 0.1O0 L 10 _ . 14.1 22.34 7.51 R733 0. 753
. 30 a I 3.06 2061 3.55 0.694 ._ 0 606 14 0 10 3 14.79 23.97 7.33 0.768 0.727 7.t 0.719 9S 6.672 11.0 20.7 0 10 30 15.10 22 04 6.94 0694 0694 3
10 9 14.8 22.10 7122 0.802 0.722 t l0 4 14.74 17.9 2.83 0.7133 .235 754 R 10 6 1463 33.23 3.S3 0597 0692 12.6 0358 55.0 0J76 19.6 iS.7 S 30 7 14.13 19.45 4.60 0657 0460
. T 10 15.06 19.07 4.01 0.802 0.401 0000o V
130 30 0
- I 000 14oo 000 O
146S 3.25 0.0 060 000 600 060.49
_-o 30.0
~ 0060 05.0 *.025 12e.6 97.t W 30 0 000 0o 0 00 0 OD . 0000 X I0 1483 11.22 0.39 0.390 .039 De.-We MSD Vllums 0.6087 MSD - Minfinm Significant Diflerce PMSV3 10.1 7MSD*- erent Minimu Sitlrcent DiffTrence PMSD i18mtsieegurcrtofi precison0The PMSD h fthe inimrWparent d (Terice between the cootrof and tnetenm thxt can be decisred stisa¶icaly Ognicanitin a whole emuent toxicity et On
-aqe sigoiantditreoast ortial TeS o hnieu 8ncind wxi-dlyiaeolhxielnt nduen i.ph.Ia . by 4.0% w c4(deeminedIhmsgh ftftewe tonks test intd Lower PMSD bourd deteraminedby USElA (I0th percentile) - 9.4%
Upper PMSD bound detemined byUScWA (90th percentile) 33%. S The lower and upper nbadswere calculated by the USEPA uing 205 tests conducbed rem I39laboreatoee fot Pimephales gsrwth in Cheonicrercrence toxicantt tks USEPA.2000. Undestnding xnd Aceeountig rorMethod Vaiability In Whole Effuent ToxicityApplieieoes Under the National Pollare Dischate Eimimetion Protv. EPA-833-R40403. US Eiemnue l Prtection Agenry, Cindnnt. OiL Oepfeni obrafeeefion Aquatic EfoSyrrew. I4c. es 7405
Environmental Testing Solutions, Inc.
Statistical Analyses LAnVI F161CrVWt andSorvll TI7 Day SorYl St3ttDUC 6/7t2005 Tes ID: PpKCt R SampleID: REF-RefToIJant EndDate 6/142005 Lab ID. E-TSRakTctiag SOL SampkeI: 9C1otasischloride Sample Date: Protocol PWCHR-EPA421-R-2-013 Test Species: PP-'imephaes prtmoli Commentr CoacImgfl 1 2 3 4 D-Conrol 1.0000 1.0000 1.0000 1.0X0 300 0.000 1.0000 1.0000 1.0000 4kO ltXO 1.0000 1AXO .OXO t00 Q1000 1.000 IDX0 Q90D0 750 0.4000 0600D0 0.7000 0.5X0 900 (X0000 0.1000 0.0X00 Q100 Trsasfo.ri Amh Squav Riot Rank 1.-TOd- Nombr Total COatc.mg/L Man N-Mm Mei Min MaT CV% N Sum Criticl Rip Number to-Con3ol l.OX 1.0000 IA120 1.4120 IA120 Q000 4 0 40 300 .0=0 1.0000 1.24120 12.420 24120 Q000 4 ISQ0 10.00 0 40 450 I.= 1.0000 IA120 1.4120 IA120 0.000 4 11.00 10.00 0 40 600 Q9250 0.9250 1-951 1.1071 IA120 21347 4 14.00 10.00 3 40
- 750 0.5500 0.5500 0.1362 0.607 0.9912 U.736 4 10.00 10.00 Is 40
- 9C0 0.0500 0.05C0 0.2403 0.1503 03218 39.161 4 10.00 20.00 32 40 AxtUw.ryTmto Stt d, Ci Skme Krt Saapk-Wiks Tt kidt noal ditrbu n(p > 0.01) 09C9913242 0.1U4 032721724 0.tC4336105 Equalty etvarinse cunnot be eoaflrmed Hlypoteis Tht(1l. L.05) NOEC WEC CbV TU Steels Many-Ome Rank Test 600 750 670.1203932 T09umentsb,DnConrol Maimnum Uikeihod-ProbWt Parameter Volvhe St 95%FtdoelUlmhz Coatrel ChLSq Crdtleut Vet1e Mu Ssma Iter Slope 27.226391292Z470121,425 12.32543951 22.06234302 0 I.639036545 7.214727703 0.65 2.372447202 0.05804277 3 Intecept .44.433346 7.155OS564 .52.4102999.30.5563922 Point Preblts m/L 95% Foad.l Luals 0i EML 2.674 546.26476 472.4191062590.51136136 EO05 3355 5933642275 541.534013 635.201092 0.2f EC10 3.71S 622.1323355 572.001364 661.9325497 o.7 ECIS 3.964 649.0519034 603.6137767 610.5U3672 Et =0 4.151 666.11173C5 6.4527391 696.1420921 a°'
EC25 4326 6SI.2311457 642.5231534 710.125631 a.5 EC40 4.747 720.67207966SI.569S797748.8651241 Ecso 5.000 745.02653 715.73746 775A421997
£C60 5.253 771.1774031 7410353099 205.003492 0.
EC75 5.674 315.1326932 703.7519175 341.2521529 02 Ea20 5..42 *34262S1I4 799.3322776MA5.W=7762 EMS . 6.036 156.27151" 3I2424216 916.1251962 0.2 EC90 6.2'2 304.7901159 U41.7694741 956396907 . 0.0 2/295 6.645 921.156832 176.6494321 1020.471647 1 t0 tWo *1000 U000 EO99 7.326 1017.394711 944.1676725 11547613 Doze nv.
peas. 13.4 Organlism obtafnedffrom Aquadk S/aSysfvvms, Inc,0-70 06407405
Environmental Testing Solutions, Inc.
Statistical Analyses Lajw.2Vleb Groth sad Serinlajet.7 DayGr..lb stul Dita: 67/2Do5 Teat 1D PpKCC Sample2ID. rFM-It T loxent Ed DataD 6MW20 LA) M. MEanvk.T.Tkg Sot Sample Type: KCLhPobuiazkchldi SampleDate: P.tocot IWCHR.EA-21-R.02-013 Tea Specics: PPPmnepbhks proamels Cooc"ngfL I 2 3 4 DCxmtrl 0.3660 0.3650 0.35.0 0i070 300 0.3640 0.7530 0.733 03660 450 0.7310 0.3230 111100 0.7530 600 0.3350 0.7130 0.6940 0.7220 750 0.2250 0.3330 0.4600 0.4010 900 0.OD0 0.0600 0.00c0. 0.0390
- Trrevr : Uatrat.rn."d I-Tab"d Istlok C..c.nlfL Mfta N.M". Mea min Max CV% N OlStt clres MSD M . N Meta D-CootI aO310 1.O00 0.4W0 Mt70 0.3660 3236 4 0.3430 2.0000 300 aun17 0.9643 o0s173 0.7530 03660 6.73S 4 0.353 2.290 0.0657 0.11173 09643 450 0.7930 0.9353 0.7930 0.7S30 0.3230 4.1M6 4 1.469 2.290 0.OS7 0.7930 0.9351 C600 0.6723 07927 0.6723 05550 0.7220 11.717 4 4.696 2.290 0.0637 0.6723 0.7927 750 0.3760 0a34 0.3760 0.2130 0.4600 19.96 4 0.3760 0.4434 900 0.024l .0292 0.0243 0.0000 0600 120.S54 4 0.024 0.0292 AnxihlwTests Stistic 0t1 ,l Stew 3.nl Shapto-wWo Test kidtet nal dotbeiOm (p > 0.02) 0.837975037 0.o44 .1.076017 0.174360589 Brutlet Test kSdbtaa equal varhnm (p 032) 3.50654t4uo I1.3446675 ItVpdbab Tat(14D 5.05) NOEC LOEC CV TU MSD MSDp 5 B033 MSX F-Prab df Dunnteo Test 4<0 600 5l9.6152423 0.033713362 0.101074719 0.0237305 0.002101792 0o.2719523 3.12 TIeseOnts n Dlcomfro LJsar ItarplblaI (2U Rc ampr .)
7.1j3 . e/L SD 95% aC XF) SkM ICos 373.64 39.73 73.6 527.7 .0.2273 ICIo 417.02 21.39 430.22 5652 0.4737 ICIS 539.69 29.44 47130 633.30 0.2776 1.3 IC20 592.36 2533 49.33 63S.93 3.397 0.9 IC25 613.3 13.73 529.70 6S344 .13441 3C40 68276 2230 643.17 72.29 .0.1449 Og:
ICSO 725.70 14.26 686.20 77+/-33 0.0623 0.7 .
- 3.3 i 0.4 0.3 0.1 0 200 400 g0o 300 1000 Dose Mwt D.-Ravpse Pl3t
.I 60.
33]
14*3, o0.0s lo 075 of slgficance as; 1e66 I .:
3.3 0.1:
OrgmniEu obtald from AqtsuaBioSystems. /no7 06407-0
IEnvironmenalTesting SolutionsInc. Page 4 of S Species: Pimephalespromelas PpKCICR Test Number: _kQ Daily Chemistry:
D.
Analyst KEt 1 7fe~ AY VL ki) ~y Concentration Parameter 9 =
CONTROL pH (S.U.) LkZ 3-*e -.4+/-a5.. -'i8.'
DO (mg/L) -0.0 (3.
Conductivity zo'0
.6(mhos/cm) 30 - 0 1_
Alkalinity L (mkg CianCtO/) _t Hardness Temperature ; . 2CA. 8 .-
pH (S.U.) - 0 Tu -4.85 -1 t-ON .1.824 l 5,26
('C)
(.5 DO (m__ _) 4 ___5 6__ _ _5_ _
300 mg KCi/L Conductivity 5145 L 5e 8 10 e
([imhos/cm) _ _ _ _ __ _ _ _
Temperature .. Zq9 2'4.. -*. l 4*
pH (S.U.) I4 e~ s -,1.S1 :nPq1.C DO (mg/L) .5 4.1.5 450 mg KClVL Conductivity 1090 1110 (pgmhos/cm) NMI I Temperature 25A 24.1 -lLA3 *-l71 -I 2.
(-C).
pH (S.U.) _ _ _ _ _1. _ _1_ (,_ 8_1
. DO (mg/L) ,@ 3_ (3S z 4LI
?0 600 mg KCVL Conductivity I I4-a 0 I qt Temperature .I l 3 ' 24 2A4-pH (S.U.) L J-41to 9-43 -1.(07o 128
-I t DO (mg/L) __9___
750 mg KCVL Conductivity (jpmhos/cm) M3d0 Temperature ZZ4. z-L -L*. b6
- _ __ _ _ _ (QC ) _ _ _ _ _ _ _2 q___ _ _ _ _ ___
pHi (S.U.) 413 :-4-(0 -4. 4 - . 6-k -
- 08 9
DO (mg/L) .t W 5.0 .{D4 .10.
900 mg KCiL Conductivity
(;im hos~cm ) 1q5 __ B o_ _______ _____
Temperature 2S.7 ZLI I zq.1 2!i.2 2q.9 24.1 S T OC K_ __ _ (' ) uIC STOCKConductivity SO K(g'mhos/cm) IO Inttial Initial I Final I I Initial I FIn1 Final IIInitial Initial Final
C agC Page 5 of of S5 . J..
Environmental Testing Solutions, Inc.
Species: Pimephalespromelas PpKCICR Test Number: jt
!Dy
^v ;Q3 4 15 6ii~l*
Uf Concentration Parameter CONTROL pH (S.U.) S .12 l_ __ 1_. tP DO (mg/L) -4.e 3.2. ? . _____9 ..
Conductivity 0 2g1 (Pmhos/cm) _________
Alkalinity (mg CaCO)L) _ l2\
Hardness (mg CaCO 3/L) a2 . gO Temperature zS.l z.3 Z7.5 2..L L'.4.a Z4.s -zq.1 Z4.L.
()_ _
pH (S.U.) n1.'q' 3.56 -4. Ld-+ - - I?.58 n DO (mg/L) -4. _ .1: 7.7 7.,F<
i - 1.3 300 mg KCi/L I S-4J Conductivity (jimho lm) ' S116 -24.51 .74 Temperature -zq.s Z.s. Z .24'i (0C) N .1 1 A
. _ _ dW fw----
pH (S.U.) 24.2q 1 ."NM .
In~
DO (mg/L) ____ '7--7 -j.0 450 mg KCiL Conductivity (jimh s/cm) 1170 3 T-.,, II-+o
. -Lq. t .
2I-4 I I Temperature
( 0C)
ZS.?2 24.9 2q.9 24LS.
pH (S.U.) _._' .oS_ 3-. .(ol -4TB . 4'S 600 mg KCi/L DO (mg/L) LI 1.
Conductivity MOO (jimhos/cm) Moo__
Temperature -s.L 2.4.1 7.q -Zq-i 2i z. J R.
__ __ _ _ ( 0c ) _ _ _ _ _ _ _-
pH (S.U.) 8.oS40 ,S5t5 OZ4-0 ? -.
DO (mg/L) 113 750 mg KC1/L Conductivity 15r0 -_
Temperature Z5 . l -IS 2 l.A 4.3 ILa .8 -
4.-. ; zq.l J pH (S.U.) 8.OL. 4.0__
_. _ +. .4qZ S-v- 1.3 1 7 Gg DO (mg/L) 0.0 -4.6 l0 . 9.9 900 mg KCVL (Conunctmi)ty 1/?20 _ _ 2_oo_
Temperature 2.S.O . l-9 NAl 7!q. Z WlSRB 2q.
STOCK Conductivity
__________(ptmhos/cm)
Initial & -
Final IInitial I L I Final IL WHOia I
.1.
Final J I Initial I Final
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.12 -- - - - - - - - - - -_
USEPA ControlLimits (I 2 StandardDeviations) 1.10 1.08 1.06 1.04 ,* ~ ~~......... . . ... . . . . .
2.5 USEPA Warning and ControlLimits (7 5th and 9ceh PercentileCVs) 2.0 I-4 .....................................................................................................................................................
Uc:
1.5 1.0 0.5 ,. ..................................
1.4 1.3 LaboratoryWarning and ControlLimits (l 0 h and 2 5th PercentileCVs) 1.2 1.1 1.0 _.._.._.._.._..._...._,_._....._,._ ._ .._.._...
0.9 .....................................................................................................................................................
0.R
.S A10, 01"-
%' t% t 0 % %
Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- -. Central Tendency (mean IC25)
- Warning Limits (mean IC2 5 ESA.X0 or SA75)
. Control Limits (mean IC2 4 SA 25 A90' or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart
'for Cerlodaphnladubla using Moderately Hard Synthetic Water State and USEPA Labortory Labentery USEPA USEPA Test nunber Teat date 7-y ICS, CT S Ceral LMnMs S ,s W.Waning Lnits S&2 5 Centra Lnits SAJI Waning Llidts Sit CeOf MULlnits CV (gQ NIIC) (g/LN CI) CT-2S CT+2S CT-SA,11 CT+SA.14 CT - SAII CT + SAM Cr - SAT CT + S.A5 CT - S Cr +SA.
1 1244-03 1.08 2 01-06.04 1.06 1.07 0.01 1.04 1.10 0.09 0.98 1.15 0.18 0.89 1.25 0,48 0.59 1.55 0.66 0.41 1.73 0.01 3 02-03-04 1.06 1.07 0.01 1.05 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 4 03403-04 1.07 1.07 0.01 1.05 1.09 0.09 0.9S 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 5 04-06-04 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 6 05-04-04 1.07 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 OAI 1.73 0.01
- 7. 06-08-04 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.1 0.89 1.25 0.48 0.59 1.55 .0.66 0.41 1.73 0.01 8 07.07-04 1.06 1.07 0.01 1.05 1.08 0.09 0.9S 1.15 0.18 0.8S 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 9 08-10-04 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 10 09-14-04 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 172 0.01 11 09-14-04 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 12 10-05-04 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01.
13 11-02-04 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 14 12-07-04 1.0O 1.07 0.01 1.05 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 15 01-11-05 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 OAO 1.73 0.01 16 02-08-05 1.05 1.06 0.01 1.04 1.08 0.09 0.9S 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 17 03-01405 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18
- 0.8S 1.25 0.48 0.59 1.54 0.66 OA0 1.73 0.01 18 0405-05 1.09 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.1 0.89 1.25- 0.48 0.59 1.55 0.66 0.41 1.73 0.01 19 05-03-05 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 20 0607-05 1.05 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 Note: I-d IC,, day 25% inlbitiou concentration. An stimaton of the concentration of sodium cbloeide that Ymuld caune a 25% reduction in Cerfodaphnfa reproduction for the test population.
CT - Central tendency (-n lCl).
S - Standard deviation ofThe nC value, Labenatexy Ceral ansd Wantin LUnits Labontory contl and wrninglimitswere etblisd using he standard deviation of th C2, values correqinding to te 102thand 25thperentile CVL Tese ranges ro more stingent than te contl and wrning limitb reconmmended by USEPA for the test method and cndpoint.
SAl, - Standard deviation eoneapouidIng to the 10" percentile CV. (SAs - 0.08)
S.29-Standard deviation conesponding to tho 25*percentile CV. (S, -0.17)
USE1'A Cenral and Waning Llnits SA7 - Standard deviation eorresponding to the 75' perentile CV. (SA, - 0.45) 6 Su - Standard deviation corresponding to the 9go percenble.CV. (SA" - 0. 2)
CV- Coefficientofvariationofthe Cn values.
USEPA 2000. Undetanding nd Accounting forMethodVariability in Whol EfMuent TosicityApplicstions Under the Ntional Pollutant Discharge Uimination Progm. EPA.S33-R-40-003. US lnvinmentl Protection Agency. Cincimati, OIL
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Tet T est date Control Control Mean CT CV CT MSD PMSD CT number Survival Reproduction for Control Mean for Control (4/) (opyring/fernale) Reproduction (%/) Reproduction (%) for PMSD (%)
(offmprinVferale) CV (%.)
1 12-04-03 100 30.4 9.7 3.6 11.8 2 01-06-04 100 30.6 30.5 4.4 7.1 3.5 11.5 11.6 3 02403404 100 30.7 30.6 7.2 7.1 2.7 8.8 10.7 4 03-03-04 100 29.0 30.2 7.8 7.3 3.0 10.3 10.6 5 04-06-04 100 28.1 29.8 6.2 7.1 2.8 10.0 10.5 6 05-04-04 100 32.5 30.2 6.4 6.9 3.3 10.1 10.4 7 06-08-04 100 32.9 30.6 6.8 6.9 3.0 9.0 10.2 8 07-07-04 100 33.3 30.9 6.3 6.8 2.6 7.8 9.9 9 08-10-04 100 27.4 30.5 5.8 6.7 2.3 8.6 9.8 10 09-14-04 100 28.7 30.4 4.9 6.5 2.4 8.2 9.6 11 09-14-04 100 28.8 30.2 7.5 6.6 3.0 10.5 9.7 12 10-05-04 100 29.6 30.2 8.2 6.8 2.8 9.6 9.7 13 11-02-04 100 30.5 30.2 8.5 6.9 2.7 8.8 9.6 14 12-07-04 100 31.8 30.3 4.9 6.7 2.5 7.9 9.5 15 01-11-05 100 31.0 30.4 9.4 6.9 3.1 10.0 9.5 16 02-08-05 100 30.0 30.3 6.8 6.9 2.4 8.0 9.4 17 03-01-05 100 29.9 30.3 6.6 6.9 2.9 9.9 9.5 18 04-05-05 . 100 32.4 30.4 6.9 6.9 3.1 9.6 9.5 19 05-03-05 100 29.9 30.4 7.0 6.9 2.9 9.8 9.5 20 06-07-05 100 30.4 30.4 5.0 6.8 2.6 8.5 9.4 Note: CV- Coefficient of variation for control reproduction On iverage, the CV for control reproduction is 6.8% in Environmental Testing Solutons, Inc. Ceriodap;hniachronic toxidcity tests.
Lower CV bound detenrined by USEPA (10' percentile) - S9%
Upper CV bound deternined by USEPA (90' percentle) - 42%
MSD - Kinimum Significant Difference PMSD - Pecent Minimum Significant Difference PMSD is a mnesurc oftestprecision. Thc PMSD is thc minimum percent difference between the control and treahfnnt that can be declared statistically significant in a whole effluent toxicity test On avrage, a significant differCce occurs for Environmental Testing Solutions, IC. chronic toxidcity tests when a toxicant reduces Ceriodaphniareproduction by 9A% fom the controL Lower PMSD bound determined byUSEPA(10 percen6ile) -1 1%/
Upper PMSD bound deternined by USEPA (90' percentle) - 37%.
CT - Cenl Tendancy (Mean Control Reproduction, CV, orPMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodap~hnia reproduction in chronic toxicant tests Tefeten=c USEPA 2000. Understanding and Accounting for Method Variability in Whole Efuent Toxicity Applications Under the Natonal Pollutnt Discharge Eimi0natin Program EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati, OIL 06-07-OS
Environmental Testing Solutions, Inc.
Ceriodaphniadubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 35- _-
E30 X:; lb 25............ .. .............
25
°320 _
U 15 ------
Minimum Acceptance Criteria (> 15.0 offspring per surviving female)
I I I I I I I I I I I I .
$ 40 ...... ____
North Carolina Acceptance Limit (< 40.0%)
<°30 ._____._.... .. . ... ............. ___.
I- s Kentucky Acceptance Limit (< 30.0%)
o a 20 U 10 ............................................................................................. ... ................. ................
00 40 I I I I LI i i USEPA Upper PMSD Bound (90 percentile < 37.0%)
30 20 10_ ................ ...........................................................
0 . I I I *. I I I I I d I Test date
- -- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- -. Central Tendency (mean Control Reproduction, CV, or PMSD)
. . Control Limits (mean Control Reproduction, CV, or PMSD 4 2 Standard Deviations)
Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia CdNaCLCR #: 36 Dilution preparationinformation: Comments:
NaCI C-M numbert _ . _ _
Stock preparation: 100 g NaCUI (dissolve S0 g NaCI in 300 ml deionized water)
Dilution prep (mtL) 600 S00 1000 1200 1400 Stock volume (ml.) 9 12 15 18 21 _
Diluent volume ImL) 1491 1488 1485 1482 1479 _
Total volume (l.) 1500 1500 1500 1500 1500 Test organism information: Test information:
Organism age: < 24-hours old Randomizing template:
Date and times organisms C S.OI.O5 lS$z.9 nlto tITO. Incubator number and were born between: __shelf location:
Organism source: Os,%-OS r A tA YCT batch: 6 lI~cS Transfer bowl information: pH =1.AA SU Temperature =ZAX C Selenastrum batch: CfS- j90S Daily renewal information:
Day Date Test Initiation, NIHS water Analyst renewal, or batch used termination time 0* ( 9 soT-OS o'.-o4-5A I 6oOt -es SA2 *-
2 Ctoc.S . 04 s.-c-OS C 3 C-O'ii-c0s 4____ 6,.-rL-CiE - ii. o06-0os A 5 s 603 13 OL 6 6 11Z 1 CarV&-QS: Z '
7 u14-cs Ob I oil1 -
Controlinformation: Acceptance criteria Summary of test endpoints:
% of Male Adults:
% Adults having 3' Broods:
4 O7.
p00%
s 20%
a 80%
7-day LC50 NOEC 7
fto lzlo
%Mortality: O . s20% LOEC /000 Mean OffspringTFemale:
I%_CV: I 1
S0. -o 15.0 offspringfemale
<40.0 %
ChV C25 1 oO.5d, t'
I : Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphniadubia CdNaCLCR N: 3Va CONTROL Survival and Reproduction Data Repiicate number Day 1 2l 3 4 5 6 7 9-8 l 1 Young produced O 0 1C 1 0 101tlOlC lC Adult mortality U U l U l L T1 .
2 Young produced l .. I. C} j. loT 1__
Adult mortality J _ l U U U t l _ U U.
3 1 Young produced O l Il I l
Adult mortality j#H1 lL 1 l 1 I . Ll L l 4 Young produced l 5 14h 5 s 3 IL sIs Adult mortality _ L-1
- 1 J1.I .
S l Youngproduced i ll l 0 I' '1lI2.lI l II Il llC) l Adult mortality j U L- - I. .- %-
6 Young produced Ql I10 O 1 .Q....l1c IOO___
Adult mortality l- - L- '-1 L-7 7 Young produced I co 'L _ 5S _I I I I Total young produced .C z'931ll 33 3 I z. 30 z8 Final Adult Nortality _ e. f t --- Cr t 7 -I X fr YdBroods C >ClCl Cl Cl ClC C Note: Adult mortality (L - live, D i- dead) 4 cp&M ott.
Concentration:
I %Mortality: I 07 Mean Offspring/Female: I 3o.-LA 600 mg NaCL/L Survival and Reproduction Data l l Replicate number r2 Day 1
I Young produced Adult mortality lYoung produced1 I.
I_
l
)
l 2
O 3
U.-.
Ol 14 D.I.I o
Cr O II
[
c 6
lL 177 O101 Tl To c l
8 U
1 I o IoIo L-9 I
. I LI 10 L-..
I Adultmortlity - !
-3 Young produced l l - I( -
Adult mortality l L- L-- UIL -
4 Youngproduced lC 5 l l 04 Sl l 4A '4 lAdult mort llty L- > l>l>ll>l>lltL-u s Young produced l- -1 iksetlr Adult mortality 1 l_. I I J L4 I. l UI- 1 - I 60P:)
6 Young produced l " l Adult mortality U-. l l_ l il l U. l __lll l 1 U-7 Young produced 1 l l 1 jj I is Total young produced l 3z 33 l31 3 l 3Z T33 l 3 T2 32 l 31 l 3Z Final Adult mlortality j L.. L U l U l Ll U l lI Note: Adult mortality (L - livc, D - dead)
I Concentration:
%Mortality: 07o l Mean Offspring/emale: I el
% Reduction from Control: l-'1.'i
Eo . -Page 3 of 6 liEnvironmental Testiing Solutions, Inc.
I -
Species: Ceriodaphniadubia CdNaCLCR M: 3t I 800 me NaCI/L Survtival and Reproduction Data
, __= Replicate number I Div I Young produced t
1C I 2 l3 C
14 4 O 5 l 6 7 8 8
9 to 10 Adult mortality L_ L L Lt . L L. L 2
.I 3 Young produced Adult mortality Young produced j
o CL L IJ .C C)
.C Q
C0 l 1L l
C.)I O
°Q l C L
C0 i
C 0
L t O
Adult mortality . L I I L L I \
1.
s Young produced 5 5 I Adult mortality lt l l 1 _l _L l ' Ll S Young produeed lj) I n l 1 II 1 JI I. I 1 U.
T Adult mortality 1- 1_ U- U-- L.
.I*.I Li L1 r 604b 7 Young produced l05Q**LO l I (oŽ1 0 L .
Total young produced lZ 8 30 31 l5z 31 I Final Adult lNtortalltv _ _l>l>l' Note: Adult mortality (L live, D d ad)
- e . .j '
- l Concentration: 'I I F nM ortality:
l Mean OffspringtFemale: l 25i
% Reduction from Control:
A l 2
Z..
o-.
l
. l 1000 mg NaCVIL Survival and ReproductionData IReplicae number
.II Day _ _ _ _ _2 3 4 5 6 7 8. 9 to l A dult m ortality l l ?l l U l . C I..
2 1Young produced 2C J Adult mortality . _ U § L . j l.
3 Young produced l l l0 Q. .0 Adult mortality l L-l LlLlU 4 Young produced JAI ll 15 L-I' s Adult mortality Young produced L-19 k.-
IC0 10 -
L I U U. L-UU L- IL.
I-Adult mortality T l-L L...l ... 4 U.
Adult mortality - ---
I -
Young produced x
l a2 a
lZ 1-2 TU.
l 2.
I U. 0T. 2U.
?-6 lZS 12.C.I LI. I U. I Total youngproduced l 3b 21 21 ( l 30 1,
- k. Final Adult Mortality Note: Adult nortality (L - live, D - dead) l - 1 i. 1-C tI L. t llL t iJT r Concentration:
%Mortality- . 6 i Mean Offspringfspnale: 21. 0
% Reduction frozn Control: t1.7-^ o S.
- .Enviri mnmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphniadubia CdNaCLCR #: ;
1200 m; NaCI/L Survival and Reproduction Data
- -Replicate number I ' Day I
l Young produced Adult mortality 1
0 1
l Q
2 l>
3 C I l
4 1 5 C _
6 l 7 L
l o
9 I o 10 2 Young produced -o - I OX O=
Adult mortality _ l L j1 l-zo 3lYoung producedf C> [ la1 O L
n I L- L- L I' 4 Adult mortality Young produced Z 3 Z s-_
.l3 JZ3 Ia-' Sl Adult mortality Young produced Adult mortality l5l L 1L>1 lC:
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l^ 4§(
L- L-l I... 6 lYoung produced lC)
_ Adultmortality L-C)
L-IO l L-
)
l Il>l°lO L
Llt-l 0 L- (-
..I. 7 Young produced l, _ l Totalyoungproduced l 17 l9z l 1 I l18 l
l3 o il i
-I l .
...oQ.. L. I o 13 l9 I.
- Final Adult lortality Note: Adultmortality (L-liveD-dead)
, t 1 l l I l l ll I I I Concentration:
l% Mortality:
07.
l I. l Mean Offspring/Female:
% Reduction from Control: la.
l -o 4,fl l
I. 1400 mg NaCV/L f 1456 Survival and Reproduction Data 1 I I a _ _ _ _ _
- - -______ ______Replicate number 1
I Young produced Adult mortality
° C 1
C l .
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. C 0
]
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IC°
_I I
2i Young produced o C:>
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l lAdult mortality ll lL-tl L 1 L-l1 t L.
3 Young produced [C) lO
[
Adult mortality . l U l- l U .I-I 1 L 4 3 13 l O Il zL - 2- -LZ-I Youngproduced Adult mortality l-l 1 L l Ul L-l UUIUIL-F5 lYoung produced lb 3 I IlIO IO I I4 l2-l Adult mortal-ty C C Ll C L- L l.-
7 6
L Young produced Adult mortality Young produced (P)=jo i
lL 1 L-i O L -*** -
- Totalyoungproduced l 5 IC l l IJO o 13 IZ Final Adult Nlortality V L i .. < l > l l Il 1l I Note: Adult mortality (L - live, D - dead)
Concentration:
% Mortality: I s7 Mean Offspring/Female: 9.8 I % Reduction from Control: j 61*. l7o
Environmental Testing Solutions, Inc.
Verification of CeriodaphniaReproduction Totals Control 1000 mg NaCI/L Day Reeplilcte number - Total Dy iRepllcate number T tal I 2 1 3 1 4 5 1 6 1 7 8 1 9 110 Dy I1 2 3 4 5 6 7 1 8 1 Tt9 1 O O O O O O 0 O O O 0 1 0 0 0 0 0 0 0 0 O o 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 6 4 5 5 5 3 4 5 5 46 4 4 3 4 5 4 4 3 4 3 4 38 5 11 0 10 10 9 12 11 11 9 10 93 5 9 10 10 11 0 9 0 12 10 12 83 6 0 10 0 0 0 0 0 0 13 24 6 0 0 0 0 10 0 10I 0 0 0 20 7 16 14 15 17 17 15 17 14 16 0 141 7 13 12 14 14 12 12 14H1 11 13 14 129 Total 31 30 29 32 31 33 31 29 30 28 304 Total 26 25 28 30 26 25 271 27 26 30 270 600 mg NaC/L 1200 mg NaCI/L Total Repcate number Total Day Replicate number 1 2 1 3 1 4 5 6 1 7 1 8 I 9 1 0 y 1 2 3 4 S 6 1 7 1 8 1 9 1 10 1 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 o o o o o 0o 0 0 0 0 2 0 0 0 °0 °0 0 0 o o 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 ° 0 0 0 0 0 0 0 0 4 4 5 4 5 5 5 4 4 4 r6 46 4 4 2 3 2 1I 3 2 3 2 3 25 5 10 1 1 10 1 111 12 10 13 9 11 108 5 7 5 0 6 0 0 4 3 4 6 35 6 0 0 0 0 0 0 0 0 3 0 3 6 0 0 10 0 6 6 0 0 0 0 22 7 18 17 18 16 16 18 15 15 15 162 7 6 8 0 4 7 7 7 44 Total 32 33 32 30 32 33 32 32 31 32 319 T'otal 171 121 21 8 11 16 131 6 113 19 126 800 mg NaCI/L 1400 mg NaCI/L Day Re picate number Total Day Replicate numberT Total 1 l2 l 3 4 5 6 l 7 8 1 9 l 10I l Y lI1 2 3 4 5 6 1 7 8 9 I lO I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0° 0 0 4 5 4 4 4 4 5 4 5 4 4 43 4 3 3 1 0 2 1 2 2 2 0 16 5 10 12 10 II 12 10 10 9 _1 11 106 5 6 3 4 3 1 0 0 5 4 2 28 6 0 0 0 0 2 0 0 0 0 0 2 6 0 0 0 0 0 7 0 0 0 0 7 7 13 14 17 14 15 16 16 14 14 13 146 7 6 1 41 5 1 6 5 2 4 6 6 3 47 Total 28 30 31 29 33 31 30. 28 29 2#1 297 Total 15 10I 10I 9 8 10 6 13 12 S 98
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphntadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNxCiCR #65 (#36 at 351 Depot St.)
Test dates: June 07.14. 2005 Revelwed by: f Concentration Rtevilexte number Survival Average reproduction cI m . I rentsatrdw a (mgfL NaCI) I i 1 5 i
6 i
7 S
9 10 (%) (offsprint/rtmale) I ,,td.ft (%) .lt t%)
I 2 1 3 Control 31 30 29 32 31 33 31 29 30 28 100 30.4 5.0 Not appUcable 600 32 33 32 30 32 33 32 32 31 32 100 31.9 2.7 .49 800 28 30 31 29 33 31 30 28 29 28 100 29.7 5.5 2.3 1000 26 25 28 30 26 25 27 27 26 30 100 27.0 6.8 1.2 1200 17 12 21 S 1I 16 13 6 13 9 100 12.6 35.7 58.6 1400 is 10 10 9 8 10 6 13 12 5 100 9.8 31.1 67.8 Dunnett's MSD value: 2.594 MSD - Minimum Significant Difference PMSD: 8.5 PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significantmina wholeeemuent toxicitytesL On average a significant differenceoccurs ror nvironrnentalTesdng Solutions Ine chronic toxicitytests when a toxicant reduces Crfodaphnla reproduction by 9.4% from the control.
Lower PMSD bound determined by USEPA (20* percentile) - I1%.
Upper PMSD bound determined by USEPA (90' percenrtile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnfa reproduction In chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability In Whole Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. l:PA-833-R-00-003. US Environmental Protection Agency, Cincinnatid OIL
Environmental Testing Solutions, Inc.
Statistical Analyses Cartedepba Servival and Repredectlen T et-Relemdetl.a l Srt Date: 6'7i2005 Teat la. CdNaCR Sample 2D REF-RefTaxicad Ead Da": 6214ZO05 Lab ID-. ETS-Envr. Testig So SamplaType: NACL-SOdium chktd Satple Dater: Protocot pWCIHR-SPA-21-R-02.013 Test Speces: CDC aiDdqhria dubi Con Cost-mIl, 1 2 3 4 5
- 7
- Jo D-Contol 31.000 30.000 29.000 32.000 31.000 33.000 31.000 29.000 30.000 21.000 600 32.000 33.000 32000 30.000 32.000 33.000 32.000 . 32000 31.000 32000 300 23.000 30.000 31.000 29.000 33.000 31.000 30.000 20.000 29.000 28.000 1000 26.000 25.000 2t.000 30.000 26.000 25.000 27.000 27.000 26.000 30.000 1200 17.000 12.000 21.000 000 12.000 16.000 13.000 6.000 13.000 9.000 1400 15.000 10.000 10.000 9.000 3.000 10.000 6.000 13.000 12.000 5.000 Tmnssadn: Uetnmatermed Rtak 1-Talled Isetonle Come-mgL M"ea N-Meae Mea Mih Max CV% N Sam Critlecl Mai N-Mean D-conrol 30.400 1.0000 30.400 2t.000 33.000 4.952 10 31.130 1.0000 600 31-900 1.0493 31.900 30.000 33.000 2.745 20 135.50 75.00 31.150 1.0000
- 00 29.700 0.970 29.700 23.000 33.000 5.510 20 91.00 75.00 29.700 0.9535 11000 27.000 0.2012 27.000 25.000 30.000 6.762 10 63.50 75.00 27.000 0.2660
- 1200 12.600 0.4145 12.600 6.000 22.000 35.729 20 55.00 75.00 12.600 0.4045
- 1400 9.800 . 0.3224 9.100 5.000 23.000 31.100 10 55.00 75.00 9.300 0.3 146 Aditary Teals statlitke Criticsl Skew Karl KoknogarovDT dicat eaoaldebutn (p -0.01) 1.06454403 1.005 0.43279813 2293S461 Bartle Teat iedktt unequal varnces (p - 4.52S45) 27.51731S7 15.0862722 HOt'els Teet(l4-WL S.05) NOFC LOEC Ctv TV SteersMamy.r Rank Teat S00 1000 *94.421191 T tb D-Cont. l Uear Inlerpolatle (203 R..amplea)
Nlbt mi/L SD S9%
CL Skew IOD 307.962963 31.7956531 73.13623 876.674107 0.164J ICI0 923.333333 33.7801856 S74.543t97 1001.53213 0.5761
.ICIS 1007.25694 1I.5625491 976.104013 1023.5413 -1.0142 1.0 1C20 1028.51939 7.57723699 1016.7497 1045.21699 0.3237 O.S
) IQ5 2C40 1050.52083 1115.41667 7.79412647 2038.17121 1067.51319 11.1700119 1097.73342 2131.2662 0.3738 0.4316 0.8 IC50 1152.68056 14.7056093 1137.47024 1119.75456 0.5602 0.0
- 500 1000 1500 Done mglL.
Dee.-Re^peme? .t 30 '
25:
Is:
- ao.
)
06-07-05
Environmental Testing Solutions, Inc.
Statistical Analyses
)
Cerlodaphale Survival and Reproduction Tedt-Reprdsetlon SWmDate: 6/712005 Test ID: CdNeCICR Sample ID: REF-RefToxicant End Date: 6n14/2005 LAbID: ETS-Envir. Testing Sa Sample Type: NACL-Sodiimnewide Saunple Dote: PMtocol: FWCHU-EPA-821-R-02413 Test Species: CD-Ceriodaphniadubia Cwunents:
COne-ni- L 1 2 3 4 5 6 7 S 9 10 D lCallm 31.000 30.000 29.000 32000 31.000 33.000 31.000 29.000 30.000 28.000 600 32.000 33.000 32000 30.000 32000 33.000 32000 32000 31.000 32000 800 2S.000 30.000 31.000 29.000 33.000 31.000 30.000 28.000 29.000 2S.000 1000 26.000 25.000 28.000 30.000 26.000 25.000 27.000 27.000 26.000 30.000 1200 17.000 3Z0C0 21.000 8.000 11.000 16.000 13.000 6.000 13.000 9.000 1400 15.000 10.000 10.000 9.000 t.000 10.000 6.000 13.000 12000 5.000 Transrorn: Untrunstormed I-TalIed Cone-mgiL Mean N-Mean Mean Mli Mao CV% N t-Stat Critical MSD DCaml 30.400 1.0000 30.400 ZS.0C0 33.000 4.952 10 600 31.900 1.0493 31.900 30.000 33.000 Z745 10 -1.322 2237 Z594 t00 29.700 0.9770 29.700 2S.000 33.000 5.510 10 0.617 ZZS7 Z594
- 1000 27.000 0.8S82 27.000 25.000 30.000 6.762 10 Z997 Z227 2594
- 12D0 32600 0.4145 12600 6.000 21.000 35.729 10 15.692 227 2594
- 1400 9.800 0.3224 9.800 5.000 15.000 31.100 10 18.161 2.287 2594 AullliaryTests Stastile Critical Sksew Kurt KclvmogarvD Test kidiea tenncsialidstrnbuton(p <0.01) 1.06454408 1.035 0.43279813 Z2938461 Bartet TeutIndirtesaunequalvaianees(p-4.52S.05) 27.5173187 .15.0862722 Hypetheds Test 1-11,0.05) NOEC LOEC ChV TU MSDn. MSDp MSB MSE F-nob df Dtnnetra Test 0OO 1000 894.427191 Z59379638 0.08532225 950.666667 6.43333333 3.2B-30 5.54 Treatmentsvs D-Cautrol
)
06-07-05
Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphniadubia CdNaCLCR #: a' Daily Chemistry:
I I-Dav I I
I 0 I 1 I 2 j Analyst _
Concentration IParameter M pH (S.U.). _ _
DO (mglL) _4 CONTROL Conductivity (umhos/cm)
Alkalinity (ma CaCO,/L) r_4
\___U7_
Hardness (mg CaCO,/L) 4 Temperature
(°C) pH (S.U.) 7 +.eD 1. 13.°ltI 1.q( 0 DO (mg/L) _ _-o T.4 9 A. Z 600 mg NaCI/L Conductivity (8mmhosacm) 1_0____
___g/L __ _____
Temperature. -z.sL 75.0
_ _ __ __2 _ _ (0 C) 2 A, ( O S .2 DO (ma/L) 0..01.0 eT9_
800 mg NaCL Conductivity S o pHl (S.U.) -8 1 19 -40 e .q 290 a~
Temperature 8 5 2z 2 m2q. o
( 0C) Z IA ___ __ _
DO (mgNL) __I_6_
1000 mg NaCI/L Conductivity 7q7 Zl0 Zi3'1 O pH (S.U.)
(pmhos/cm) ]6,.O _ )6 _ _ _ .- 1t-[15
____3 ___
Temperature Z. 2S. 2q.q %5 . ? -LS.
pHS(S. U.dL Iq 1.1"X i i.'lt 1200 mig NaCI/L Conductivity 7
( pltm h os/cm) _ _ _ _ __ __ _ _ _ _ _ _ _ _ _ _ _
Temperature zALq250 -ZS.3 2A.'- Li -S.
__ __ _ _ _ _ _ _ _ _ ( 0C )_ _ _ _
DO (mg/L) 9-1400 mg NaCIIL Conductivity 90W0 (g~m hos/cm)__ _ _ ___ _ _ __ _ _ _
Temperature z'-~2L- -L ~ ~ .
STOCK Conductivity ~ 9o _ _
Inldial Final Initial Final Initial Final
______ L -
Environmental Testing Solutions, Inc. Page 6 of6 Environmental Testing Solutions, Inc. Page6o6 Species: Ceriodaphniadubia CdNaCLCR Xie: k3o
. 45 34 5 6
.Analyst Y*= KEA hty 1 lt hi>
r*1 Concentration Parameter pH (S.U.) _ _ .7 _ ____ 5._
DO (mg/L) 0 .5 .1 1.
CONTROL Conductivity 3Q0 L -27 (gmhoslcm)
Alkalinity (mIg. CaCOV3L) __1___
Hardness (mg CaCO 3/L)____
Temperature .
_ (°C) ZQ1 -I 5. - 20 2c4L2 q ,cW
.l.pH (S.U.) -P.o % * +0 3.e l.86 7. 17 1.nN , 7 43
! 600mg NaCI/L
____(
DO (mgtL)
C Temperature 0
C) v(it /4 el,I
.1 8-2.
13;o .
7A SI
-8.8I i'O pH (S.U.) E4.Q7. UJ. 1-L 8 1 .XL 0 ?-C a DO (mglL) 8,2 B, 02 I f0n 3 n .
- 800 mg NaCI/L Conductivity 750 0 1*00 (jimhos/cm) __ _ __ __ _ ___ __ _
Temperature 1z'u zS.4 24A lq.q 2 2S.1 Zq1 *l2 0c)
PQ( __ __ _ _ ___1_
pH(S.U.)
DO (mglL) 0.0O A
8 0I31 3¶
- 1 1.1 ,80 7 2 1000 mg NaCI/L Conductivity /70 22e0 z3 O )
Temperature vg 2 Z 2S. I 2Y 3 S0 1__
_ __ _ __ _ _ _ _ _ (0 C) _ _ _ _ _ _ _ _ _
DO (mg/L) __O .3 1200 mg NaCIJL. Conductivity -f pH (S.U.)
(ipznhos/cm) a520J.tO0z -4 e } ______ 4 . 7 _____o .Q 1. -7__00
'.4 7 G Temperature ?q.(o 2l3 2. Z'.l 0 zq7 zq - l (OC) ___
140rgNCLDO (mg/L) *.9.-3j~ -I -u. I.-I 7 10mgNC Conductivity 7 z'io__
7.'
B ITemperature
_____(
0c)
Z2
- z.
Z za(S2- ~ 24/)S.-.z.
2.- -.
OCK Conductivity - __ __
Iniltial1- 11Final I Tnit;al
- I rn-al Fi - I Tnitil I r FVinal
- I Tnt0*1 r IJ
- -. J Iin
PERMITTEE NAMEIADDRESS (Incdude Facilitv Namemocation ffDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMB No. 2040-004 Address P.O. BOX 2000 ___________
_ _ _ _ffEEROF9lCE!LB-?_ _- -- - I TN0026450 II 103 G I s F-_FINAL 1 I DISCHARGE NUMBER l l SODDY. DAISY_ TN 37384 I l---
PERMIT NUMBER l LOW VOL. WASTE TREATMENT POND Fdacliity A - SEqUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ------ --- MONITORING PERIODjj EFFLUENT YEAR IMO I DAY IYEAR IMO DA
... NO DISCHARGE = **
ATTN: Stephanie A. Howard From 05 1 06 l 011 To I05 106 30 NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_ _ _ _ _ ___ _ _ _ _ _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANAL.YSIS PH SAMPLE ** f2 7.0 0 9.0 13/30 GRAB MEASUREMENT 26 19 00400 1 0 0 . , RT ,_,_-.m -:A***MX'LDM .
OMEAREEEN AND C6GRAB GREASE EFFLUENT GROSS VALUE_________________________MINIMUMA REQUIREMENT ,, rZ~~ MAXIMUM ____ .
TEEK WE GRAB SOLIDS, TOTAL SUSPENDED SAMPLE 7 1 7 10 1 0 5/30 GRAB 00556 1 0 0 8WPRMZ~-
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-ormJ3ZU1 tKV uecPPge 1of NAME/TITLE PRINCIPAL EXECUTiVE OFFICER I Certify wider penaltyof law Vial thib document and all attarhinents ware prepared under my t , .,/ TELEPHONE DATE diredtion or supervision in accordance with a systemn desIgned to essure that cqualified personnel j~ J-' / i C J. Randy Douet properiy gather and evahuate UheIntonniation aubmined Based on my Inqtuiry of the person or . 'r SifVintl^ on. the Informaloon submit ed Is .tothe bes0tofmykoege and bi true Principa tal Engineer 423 843-6700 05 07 14 Sie Vce President ac curate, and corniplete, I am aware that there are significant penalties for subminling false SIGNATURE OF PRINCIPAL EXECUTiVE 1
TYE.RPRNE nfomitation. Including i,.posaibtiiyoffine and in-prisnment forknowing vioathons OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLAT iONS (Reference aOfattachments here) .CD tt-A F-orm 3320-1 (KtV 3199} Previous ecritionsmay oe used Page I of I
PERMITTEE NAMEIADDRESS (include FacllityNametocation if DifferentJ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 __________
_-_ _N _fEROffl(E!LBA-2) - -_ I TNO24 l 107 G l F- FINAL ODY - DAISY TN 37 84 -_ I PERMIT NUMBER I UMBERJSCHRGE METAL CLEANING WASTE POND acity_ TVA - SEQUOYAH NUCLEAR PLANT ---
Location HAMILTON COUNT---------- l B i MONITORING PERIOD 1 EFFLUENT l-* s s ! ss-YEAR l MO DAY I YA E NO DISCHARGE ATTN: Stephanie A. Howard From 105 1 06 1 01 ITo 11051 061 30NOTE: Read Instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12*****
MEASUREMENT ** 12 00400 1 0 0 : CPERMIT: .`! - 6 '_ S
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EFFLUENT GROSS VALUE UREEN2'R ~ _4 t IIMM . MAXIMUM PHOSPHORUS, TOTAL (AS P) SAMPLE ******** ******** ******** ****** 19 MEASUREMENT 00665 1 0 0
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SOLIDS, TOTAL SUSPENDED SAMPLE ecceece. *********** ******** ***** 19 MEASUREMENT 00530 1 0 0 ,PERMIT-.Tt- ' 30 MGIL . DAILY-- COMPOS EFFLUENT GROSS VALUE DEAEENW..'~.AL Ym~t____. ___
OIL AND GREASE SAMPLE ******** ******** ******** ******** 19 MEASUREMENT 00556 1' 0 0 ~ PERMIT.T--~; i --- '**~* ~****7 K~25- tt*****-MG/L ~- DAILY,.. GRAB~
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- REQUIREMENT,.'
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______ , DAIY ML X FLOW, IN CONDUIT OR THRU SAMPLE 03 ********
TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 :PgDAERMfL4Y% RP TOT REP,,meArRT.-,- ...-` *,REP0R MGD _ . .-DAILY::;. CALOTD EFFLUENT GROSS VALUE , 24 _ 2,- MO'AV- DAILY M X,* __________ r ~*
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this docinent and an attachments were prepared under ry // I TELEPHONE DATE J. Randy Douet direction or supeivslon in accordance with a system designed to assure thaI qualified personnel properlygatherandevatuatetheinformnatonsubntted.BasedonrmyInquiryof thepersonor, g.te L. )C persons who manage the system, or those persons directly responsIble for gathering the Principal Environmental Engineer 423 843-6700 05 07 14 Inforrmation. the Information submItted Is.*to the best of my knowledge and belief, true.
Site Vice President accurate, and complete. I am sware that here are significant penalties for subrritting false SIGNATURE OF PRINCIPAL EXECUTIVE inforniation. Induding the possibility of tine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ICODE COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference all lttechments here)
No Discharge this Period
~
EPA~ (REV 330.
EPA Form 3320.1 ~
(RV39)
W99) For editions Pe.u Previous dton maybe ab used sdIaeio Page 1 Ot I
PERMITTEE NAME/ADDRESS (Indude Facilitv Namne/ocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MMOR Form Approved.
Name _ VA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 (INTEROFFICE B- -- I TN0026450 ] 110 GI F- FINAL SODDY - DAISY T 37384 I PERMITNUIMRFR I lDISCHARGENUMBERl RECYCLED COOLING WATER Fcity _ A - SEQUOYAH NUCLEARPANT Location HAMILTON COUNTY EFFLUENT
- NO DISCHARGE E1 **
ATTN: Stephanie A. Howard From0 0 (1I To 051613 NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
______ EX_____ O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT 12 00400 1 0 0 -~4PERMITo~zA- *-. 0.,;C7~90 DAILY
- e** ~GRAB EFFLUENT GROSS VALUE T _
~REQUIREMENT-~~
~MNMUr--
_':/MINI________ _;_su_'_--i___iGA8' SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 0 _ PERMIT=.-' ._ hr . ^ 30 MGIL -,DAILY: COMPOS EFFLUENT GROSS VALUE . _. _ DAILY MG L ____'CP OIL AND GREASE MEASUREMENT
- 1 SAMPLE 19 MEASUREMENT 19 00556 1 0 0 ii: PERMIT. MGL
^ * , 1MDAILY COMPOS D104510. AILY MX -L,'DAILY CPHPOR, TOTAL (ASU) SAMPLE
- MEASUREMENT 19 EFFLUENT GROSS VALUE ~ EURM~.* D IYM EFLETGOSMAU EASUREMENT ,.,~ .~zr 19*...
- AKL;o~I-; 19 MG1iL -C!, r`DAILY~ COMPOS EFFLUENT GROSS VALUE ~-~
MEASUREMENT ~'<~LMLII ___ _______
PHOSPOUSJOTAL 01042 1 0 0(AS ) PE v SAMPLE
- PERMI2WAj*.**-t!. ' _~. 2:: t 1 t0.- t* 19 MGRL r'. DALCM MEASUREMENT 010665 1 0 0 PR T" -i**.**.Y. *K? -ZAI O: MG/. -DAILY COMPO YREOUIREMENV.,,
EFFLUENT GROSS VALUE .*-' ________ DIYMX _____-
FLOWPNHONDUIJTA ORASRU SAMPLE TRAMN LfTMEASUREMENT 03 500650 1 0 0 :PERMIT,4~:, DAlLYi:.,
-4RPRT..- CALCOS
-~'-i~-~4 DILY ~M MX'; AVG ______ ~ DAILY M ____ ,
EFFLUENT GROSS VALUE REQUIREMENT-'.7 ERTD2RER MGD _ .. D _
NAMEATITLE PRINCIPAL EXECUTIVE OFFICER I Cerfy under penalty of law that thi document and all attac0ments wns prepared under my TELEPHONE DATE J.Randy Douet pOCRIerty gather andevaluateate Infomation submittead ase cmeyon Inquirydf the person or I L DATE persons who manage the system, or those persons directiy responsible for gathering the SitecePresident nformation, the Information submitted Is. to the best of my Inowledge and belief Pinc alEnvinmentalEnginee 423 843-6700 05 07 14 StViePeietaccurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPEDORPRINTED information. Induding the possibilityof fine arndImprisonment for knowing vdolations. OFFICER OR AUTHORIZED AGENT AREAj NUMBER YEAR MO DAY TYPED OR PRINTED ________________ CODE_________________________
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period
- m. use Pag 1 of. 1 EP For 330. (RE_ru 319 e.tin EPA Forrn 3320.1 (REV 3199) Previouseditions may be used Page I of I
PERMITTEE NAMEIADDRESS (Include FacilitYName/location ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT _ DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0 004 Address P.O. BOX 2000 __________
_ __ _LINTERO9q1E aB-2@ffi__-
SODDY - DAISY TN 37384 l
TN0026450 PERMIT NUMBER
!1 116 G I I DISCHARGE NUMBER
! F - FINAL BACKWASH Fadciy_ TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY __________ MONITORING PERIOD EFFLUENT ATTN: Stephanie A. Howard From 05_
MO I DAY I 06 1 01 I Tol 05 I MYER DAYM
_06 30 *** NO DISCHARGE D **
NOTE: Read Instructions before completinca this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
__ __ _ _ _ _ _ _ _ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1/30 VISUAL MEASUREMENT 01345 1 0 0 *trPRI.~~...~1/2RPR- PASS=O -SE~-VSA EFFLUENT GROSS VALUE REQU14EME. : y v ' ' MO TOTAL FAIL:S OIL AND GREASE VISUAL SAMPLE 0 94 0 1/30 VISUAL MEASUREMENT 84066 1 0 0 -U 14EMIT REPORTQ-,t .* YE=C YE- _ .SEE" VISUAL-EFFLUENT GROSS VALUE &RQIEET OTTL NO=0 LOrr.~PRI SAMPLE MEASUREMENT
.7 W MREASUREMENT~ X .. .-.-
SAMPLE MEASUREMENT PERMITar~ $I-_ * .i*-'.
,4REQ=UIJEMENT .N i kr' -,...... . -
SAMPLE MEASUREMENT 2~PERMIT, ~ rN.~ ~
.:REQUIREMET ~ :;x~
- ~***~
SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Celtify under penalty of law that this document and all attadhments were prepared under my . /1// / TELEPHONE DATE uirecionorsupervisioninaccordancewfth asystemdesignedtoessure that qualifiedpersonnel flh Q I,(o /v .. d_
J. Randy Douet properly gather and evaluate the Information subrdtted. Based on my Inquiry of the person or I persons who manage the system, or those persons directly responsible for gathering the Principal Environmental EngIneer information, the Information subrnitted Is. to the best of my knowledge and bellef, trne, 423 843-6700 05 07 14 Site VIce President ascurate, and complete. I am aware that there are significant penalties for subnittlng false SIGNATURE OF PRINCIPAL EXECUTIVE TYE RPITDinformation, indluding the possibilityoffine and Imprsonment for knowing viodadbns. OFFICER OR AUTHORIZED AGENT. AREA I NUMB3ER YEAR MO DAY TYPED OR PRINTED I____________________________ ________________jCODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference aal ttachments here)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
____QE NTRFIE!18-?
0 _ ____ I TN0026450 17LG F - FINAL SODDY - DAISY TN 37384 I PERMIT NUMBER [DISCHARGE NUMBER BACKWASH aciity_ TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY irmin%7iTOrIN =PFIOD 1 EFFLUENT FYEARoI MO I AY YEARM DAY *" NO DISCHARGE F -
ATTN: Stephanie A. Howard From 105 1 06 1 01 To 0O5 106 130-NOTE: Read Instructions before comoletina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
__ _ __ _ _ _ _ __ _ __ _ __ _ _ __ _ __ _ __ _ _ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A o 1/30 VISUAL MEASUREMENT 01345 1 0 0 - tiPERMIT;2 ;--*** ****i . .,.' REPORT- PASS *= SEE' 0 :.*** LVISUAL EFFLUENT GROSS VALUE REURM f ri
.- v ?'7 -FAIL=1
.MTOTAL' ERMIT ~
OIL AND GREASE VISUAL SAMPLE 0 94 0 1/30 VISUAL MEASUREMENT 84066 1 0 0 -JPERMI`17',-i.v E= *-i'-,,e~ '-SEE;t.- : IVISUAL.:
EFFLUENT GROSS VALUE M, N_ OREPORT R-0 PERMIT' SAMPLE MEASUREMENT SAMPLE..
MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT "ARfOUIREMEN-0 W '.
A E<.ERi,.,>- , a-- > -- *~ =.--.. .. ..
'-- SAMPLE PEPMiT -r .....
.'; . .: ^* :
..... ffi_
1,,f ^1 MEASUREMENT YREOUIREMEN ~ . - ,. .,.- W - ".:
NAME/TITLE PRINCIPAL EXECUTiVE OFFICER I Ceatily under penaity of law that this document and all attacihments were prepared wider my D/ 2. i TELEPHONE DATE JirectionorsuperisionInaccrdancwithas ytem deslgned'toassurethatqualifiedpersonnel 44 .f^ 1 OI" J. Randy Douet propedy gatherandevaluate the Information aubnstted Based onmy Inquiry ofthle erson or_w Si. Vie reeen iimon. the Informathon subtm itotehIostothe best of my knwedge and belief true Prindpa Eniomntai Engineer 423 843-6700 05 l07 l14 S Ite VicePresidentaccurate, and coniplete. am aware that there are significant penalties for aubmnsttngi false SIGNATURE OF PRINCIPAL EXECUTiVE TYPED____
OR__PRINTED _____nformalion, including the posibility of fine and lngntsonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUBE YEAR lMO lDAY AND EXPLANATION OF ANY COMMENTS EAFr3301RE3/9 VIOLATIONS (Reference PrvoseItionsmaybnte all usedmaio here) attachments ele. re Pagete 1.totebetofm of 1dean Operations performs visual Inspections for fioating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199) Previous editionsmay be used Page I of 1
PERMITTEE NAMEIADDRESS (indude FecItv Neme/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name _TA - SEQUOYAH NUCLEAR PLANT ____ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) 0MB No. 2040-0004 Address P.O. BOX 2000 ____________
__ _ _ffjLEaOfflE _ 2_ 1 TN0026450 118 G l F- FINAL
_ _ _ _SODDY D2AISY_ TN 37384 - - - - I PERMIT NUMBER I I DISCHARGE NUMBER WASTEWATER & STORM WATER aciitv_y VA- SEQUOYAH NUCLEAR PLANT _
Locaton HAMILTON COUNTY ----- FmNIToRING PERIOD I EFFLUENT ATTN: Stephanie A. Howard From YEAR I MO I DAY~
05 l 06 l 01 I To I YEAR 7IOl 105 l 064 301 A
"' NO DISCHARGE [1
- NOTE: Read instruclions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
__ _ __ _ __ __ __ _ ___ _EX__ _ O TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE *******
MEASUREMENT 19 00300 1 0 0 .- PERMrnO2 r *** ** *
- A > ..r^ * - MGIL : -TWICEI 1GRAB -:
EFFLUENT GROSS VALUE ' L '..C"A - - -. WEE -
SOLIDS, TOTAL SUSPENDED SAMPLE ******** ***** ********
MEASUREMENT 19 00530 1 0 0 L1/2PERMI T
'********.1. - - 0AB0 OC k G*****R*A*
B*****
EFFLUENT GROSS __ VALUE
_ __ __ _ -_ REQUREM, , ., DAIY. __
_ DAILY MX* MG - ,WEK -
GRoB SOLIDS, SETTLEABLE SAMPLE ******** 25 MEASUREMENT 00545 1 0 0 !:.,PERMW -. .****-~.,..ML*******CE.V ***G(RA*****
EFFLUENT GROSS VALUE -REQUIREME7 f:.-Lt r.~~~-~
"-DIYM
~ 10 ~LOCI
~MONTH- GA 7z.4 FLOW, IN CONDUIT OR THRU SAMPLE 0 TREATMENT PLANT MEASUREMENT 50050 1 0 0 PRI- 'RPOT2 MGD **4--****,..,'- ONCE/-- ESTIMA, EFFLUENT GROSS VALUE REQUIREMEN6
~ -- ~LREOT~'
D
'jAILYWW"' ~- BATCH:___
SAMPLE MEASUREMENT
- REUIREMENT: REPRT'-t~ 4'r R E P0 RTfs -w 3. ;:8 SAMPLE MEASUREMENT zPERMIT,..-*' *****> -.
SAMPLE MEASUREMENT
.~L~t-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my . // TELEPHONE DATE direction orsupervisionIn accordance Willasysterndesigned to assure that qualifiedpesne W 2t liZ^ 0/ (w f ,
J. Randy Douet property gather and evaluate the Inforriation submitted. Based on my Inquiry of the Person or , T Site President Vice persons wto ebnage the syster% or those persons directly responsible for gathering the i~nformation, the Information submitted Is , to the best of my knowledge and befief. true.
Principal Environmental Engineer 423 843-6700 05 07 14 Ste Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTED TYPED OR PRINTED Itinfomnation. Incuding the possibilityof fine and Imprisonment for knowing violations.
OFFICER OR AUTHORiZED AGENT AREA CODEL NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference atl attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EP Fo_ 33_- (RE 3_9 _ v edti. m.b use Pag 1.of 1 EPA Form 3320-1 (REV 3199) Pravtouseditionsmaybe used Page I of I