Text

Discharge Monitoring Report for February 2005
	ML050760383
Person / Time
Site:	Sequoyah
Issue date:	03/14/2005
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML050760383 (117)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 March 14, 2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2005 Enclosed is the February 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, SiCA. 401,4-Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk ED0= Washington, D.C. 20555

PERMITTEE NAME/ADDRESS (Indude Facility Name/Location if Different) Namen TVA - SEQUOYAH NUCLEAR PLANT ____ Address P.O. BOX 2000 {ITEROFFICE SB -?N - SODDY - DAISY TN 37384 FcilityTVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN026450 l101 G F-FINAL I PERMIT NUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE I MONITORING PERIOD I EFFLUENT Forrn Approved. OMB No. 2040-0004 I YAR O DYJ YEAR MO DA From 10j1 21 0 .TOL 01021 8" NO DISCHARGE E i.. NOTE: Read Instructions before completing this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 10.3 04 0 28/28 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 '4-:.PERMIT~- 3f****\\q~

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~ ~ 03 008MG/L WEK-CALCTD-EFFLUENT GROSS VALUE 0_ 0_ _a_ M G I L _,CLT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my l ^ ( TELEPHONE DATE direction or supervision In accordance wvith a system designed to assure that qualified personnel J. Randy Douet propertygather and evaluate the information submitted. Based on my Inquiry of the person or )bt, _ d i V P dInformation, the Information submitted Is, to the best of my knowledge and belief, true _nmental Enginee 423 843-6700 05 03 11 Site Vice President accurate, and comolete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TYPEDORPRINTED _information, Including the possibilityof fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference ae teachments here) No closed mode operation. The following Injections occured: 1. PCL-222 (max. calc. conc. was 0.028mg/L-limit 0.100mg/L) 2. Biodetergent 73551 (max. calc. conc. was 0.007mg/L-limit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.01nmg/L-limit 0.100mg/L) EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2

PERMITTEE NAME/ADDRESS (Inctude FacilitvNameltocation ifDifferent) Name TVA - SEQUOYAH NUCLEAR PLANT ___ Address P.O. BOX 2000 ____ilNqTEROFFICE B-UA )__-_--_-_--_-_ SODDY -DAISY TN 3734 Facility_ TVA-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0026450 101G F-FINAL PERMIT NUMBER I DSCHARGE NUMBER DIFFUSER DISCHARGE I -MONITORING rPERIOD EFFLUENT Forn Approved. OMB No. 2040-0004 Fm MO DAY [YEAR ITMO DAY From 05 02 1 01 ITo 25 0228

- - NO DISCHARGE D

NOTE: Read Instructions before completing this form. NAMElTiTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty ofdaw that this document and aii aitachrnents were prepared under" 1 TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified persornref ,.o ( -Ji G2.I persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 03 Si. \\lice President information. the Information subrnited Is, to the best of my knowledge and beiier. true. Prncpa______ _Egme 423 843_6700 05_l_03 11 Site Vice President accurate. and complete. I am aware that there are significant penalies for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED_ ORPRINTEDInforrnation. Including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _ I _ CODE COMMENTS AND EXPLANATION OF ANY VIOLATiONS (Reference el afttachments here) CCW data for February 2005 Is attached. EPA Form 3320-1 (REV 3199) Pmvibus editions maybe used Page 2 of 2

CCW TRENCH DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 02/08/2005 0905 <0.5 mg/L 02111/2005 1930 CVS EPA 8015B CCW CHANNEL DatelTime Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 02/08/2005 0910 <0.5 mg/L 02/11/2005 1852 CVS EPA 8015B

PERMITTEE NAMEIADDRESS (Include Facility Name)Loceiion if Different) Name TVA - SEQUOYAH NUCLEAR PLANT________ Address P.O. BOX 2000________ _L1?,E9EEO E i SODDY-DAISY TN 37 384 FacdLty v A - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For DISCHARGE MONITORING REPORT (DMR) (SUBR 01) ON I TN0026450 101 T F - FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 I ,MONITORI G PERIOD 1 EFFLUENT mn Approved. lB No. 2040-0004 I I YE -M A O I DAY From 05 L 02 j01 I To 05 l 02 L 281

- - NO DISCHARGE I--

NOTE: Read Instructions before completing this forn. I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my ,i I TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel _L n1 (QtA_ J. Randy Douet property gather and evaluate the Information subnmited. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 05 03 S t V-P information, the Information submitted Is, to the best of my knowledge and bellef. true, ___nvonen_.__ngeer___________42_______5_0_1 Ste Vice President accurate. and corrplete. I am aware thal there ar significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Information. Including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a attachments here) Toxicity was sampled February 6-11, 2005. Report is attached. EPA Form 3320-1 (REV 3/99) Previous editions maybe used Page 1 of 1

March 8, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, FEBRUARY, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected February 6-1 1, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment): Files, R&TA, CTR IB-M SQN February 2005M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: March 8, 2005

1. Facility / Discharger: Seguovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3.266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQ10: 2.992.4

8. Outfall Tested: 101

9. Dates Sampled: February 6-11. 2005

10. Average Flow on Days Sampled (MGD): 1477. 1477. 1475

11. Pertinent Site Conditions: H-1 30M molluscicide was not injected from February 6-11. 2005.

12. Test Dates: February 8-15, 2005

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimeyhales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98, 22.0. 43.9. 72.0. 100.0 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: ICs, = 43.9%

17. Test Results: Outfall 101: Pimephales promelas: IC, > 100%

Ceriodaphnia dubia: IC?5 > 100% UV treated Outfall 101: Pimephalespromelas: IC5 > 100%

18. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6700

19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected February 6-11, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples. 2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received UsB (mg/L) y 101 02/07/050832 02/07/05 1350 0.9t <0.10 02/08/05 1348 Intake 02/06/05 1050 to 02/07/05 1350 1.3 <0.10 02/08/05 1348 02/07/05 0950 02/09/05 1318 101 02/09/05 0659 02/09/05 1343 2.9, 17t <0.10 02/10/05 1329 Intake 02/08/05 0822 to 02/09/05 1343 1.3 <0.10 02/10/05 1329 02/09/05 0722 02/11/05 1321 02/10/05 0858 to 02/12/05 1258 101 02/11/05 0758 02/11/05 1313 3.4, 3.1 t <0.10 02/13/05 1254 ____02/14/05 1302 02/12/05 1258 Intake 02/10/05 0844 to 02/11/05 1313 0.8 <0. 10 02/13/05 1254 02/11/05 0744 02/14/05 1302

TRC = Total Residual Chlorine

+Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Samnple Manipulation: Samples from Outfall I101 and intake were warmned to test temperatur (25.0 -1.00C) in a warm water bath.

Aliauots of Outfall 101 and Intake samnles were UV-treated throueh a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes. 3

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source:

Aquatic BioSystems, Inc. In-house Cultures

2. Age:

21.75-24.75 hours old <24-hours old Test Method Summary:

1. Test Conditions:

Static, Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water:

Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates:

5. Organisms per Replicate:

4 10 10 I

6. Test Initiation: (Date/Time)

Outfall 101 02/08/05 - 1348 ET UV Treated Outfall 101 02/08/05 - 1330 ET

7. Test Termination: (Date/Time)

Outfall 101 02/15/05 - 1300 ET UV Treated Outfall 101 02/15/05 - 1241 ET 02/08/05 - 1116 ET 02/15/05 - 1118 ET

8. TestTemperature:Outfall 101:

Mean=24.8C (24.3 - 25.4 0C) Test Temperature: UV-Treated Outfall 101: I Mean = 24.80C (24.3 - 25.30C) Mean = 24.80C (24.1 - 25.50C)

9. Physical / Chemical Measurements:

Alkalinity hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for DH and dissolved oxvgien.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinneyville, CA). 4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted February 8 -15. 2005 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 98 98 98 22.0% 100 100 100 100 100 98 98 43.9% 100 100 100 100 100 100 100 72.0% 100 100 93 88 88 83 80 100.0% 100 100 100 98 95 93 93 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg) (% Effluent) (rep icate numbe) Mean 1 2 3 4 Mean Control 0.646 0.607 0.631 0.697 0.645 10.98% 0.620 0.616 0.686 0.692 0.654 22.0% 0.618 0.653 0.748 0.664 0.671 43.9% 0.709 0.621 0.640 0.626 0.649 72.0% 0.718 0.586 0.195 0.681 0.545 100.0% 0.581 0.438 0.598 0.664 0.570 Intake 0.645 0.590 0.706 0.654 0.649 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA LowerLimit: NA

TUa = I 0O/LCso: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic! 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted February 8-15. 2005 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) S(ofluetio 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) (%flet I F2 3 4 5 6 7 8 9 10 Mean Control 29 30 27 30 28 34 32 31 30 30 30.1 10.98% 31 36 32 29 32 31 34 34 31 1 27 31.7 22.0% 32 35 33 35 32 32 31 32 30 28 32.0 43.9% 32 36 36 34 34 37 34 34 31 37 34.5 72.0% 33 37 36 31 32 36 37 33 36 35 34.6 100.0% 35 35 38 35 34 38 35 37 37 34 35.8 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = I 00/LC5 O: TUc = 100/ IC25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicitv Test. (Genus species) (Type / Duration) Conducted February 8-15. 2005 using water from Intake Percent Surviving Test (time interval used - days) (oEffluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 I100 I 100 I 100 l 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) I 2 3 4 5 6 7 8 l 9 10 Mean Control 31 28 30 30 29 30 26 31 32 29 29.6 Intake 31 32 34 34 32 33 32 31 33 35 32.7 IC25 Value: > 100% Calculated TU Estimates:< 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = I00/LC50: TUc = 100/ IC25 7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted February 8-15, 2005 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 98 98 98 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 98 98 98 98 98 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg) (%Effuent) (replicate number) (HO Efun) 1 l 2_l_ 3 4 Mean Control 0.632 0.738 0.699 0.711 0.695 10.98% 0.738 0.743 0.710 0.585 0.694 22.0% 0.659 0.647 0.698 0.700 0.676 43.9% 0.627 0.706 0.698 0.673 0.676 72.0% 0.577 0.692 0.567 0.622 0.615 100.0% 0.719 0.575 0.547 0.706 0.637 Intake 0.634 0.707 0.624 0.675 0.660 IC25 Value: > 100%/o Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = I00/LC5o: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species I Date Time Duration Toxicant Results (OC25) Pimephales promelas I February 8-15, 2005 1441 7-days I KCI 0.62 g/L Ceriodaphnia dubia February 8-15, 2005 1103 7-da s NaCI 1.05 g/L 8

PIIYSICAL/ClIEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for lintephales promelas and Ceriodaphnia dzubin Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, February 8-IS, 2005. Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pil SU.) Conductance Alkalinity hardness Total Residual Initial Final Initial Final Initial Final (jzmhoslcm) (mg/L CaCO3) (mgIL CaCO,) Chlorine (nmgIL) Control 25.1 24.6 7.8 7.5 7.88 7.59 295 61 84 24.9 - 25.3 24.5-24.8 7.7 7.8 7.1 7.7 7.72-8.11 7.39-7.85 282 308 58 64 8I 86 109% 25.1 24.5 7.9 7.5 7.87 7.58 284 109% 24.8 25.2 24.3 24.6 7.7 8.1 7.2-7.7 7.61 - 8.22 7.41-7.85 271 304_______ 25.1 24.6 7.9 747.87 7.8277 2.% 24.8 - 25.3 24.3 24.8 7.7-8.2 7.2 7.6 7.62 8.21 7.42 - 7.84 265 -28______________________ Q. 4.% 25.2 24.6 7.9 7.4 7.87 7.59 254 ar_24.8 25.4 24.4 24.8 7.7 8.2 7.1 7.6 7.61 8.23 7.42 7.87 240 288 -Z25.1 24.5 7.9 7.4 7.85 7.61 218 72.0 24.9 25.3 24.3 - 24.7 7.7-8.3 7.1 7.6 7.57-8.23 7.46 - 7.85 205 255 100.0% 25.2 24.5 7.9 7.5 7.86 7.64 187 67 80 <0.10 25.1 - 25.4 24.3-24.8 7.7 8.3 7.0 7.6 7.59 - 8.25 7.48-7.91 174-225 66 68 78 82 <0.10- <0.10 Intake 25.1 24.5 7.9 7.4 7.86 7.62 189 67 79 < 0.10 25.0 - 25.3 24.4 - 24.7 7.7-8.3 7.1 7.7 7.61 8.27 7.49 - 7.87 173 223 66 68 78 80 <0.10 - <0.10 Control

25.

24.6 7.8 7.7 7.88 7.76 295 61 84 24.7 - 25.3 24.4 - 24.9 7.7 7.8 7.4 8.0 7.72 '- 8.11 7.57-8.13 282 308 58 64 SI 86 109% 25.0 24.7 7.9 7.8 7.87 7.76 284 109% 24.7-25.2 24.5 - 25.1 7.7 8.1 7.4 8.1 7.61 8.22 7.59-8.12 271 304 22.0% 25.0 24.6 7.9 7.8 7.87 7.76 277 24.7 - 25.1 24.4-25.1 7.7 8.2 7.5 8.1 7.62 - 8.21 7.58 8.11 265 298 Z25.0 24.7 7.9 7.8 7.87 7.77 254 _____24.6 25.1 24.4 25.0 7.7-8.2 7.3 8.1 7.61 8.23 7.59 8.12 240 288 7.% 25.1 24.7 7.9 7.8 7.85 7.77 218 72___0_ 24.7 25.3 24.3 25.2 7.7 8.3 7.4 8.1 7.57 8.23 7.59 8.14 205 255 10.% 25.0 24.6 7.9 7.8 7.86 7.79 187 67 80 <0.10 24.5-25.3 24.3-24.9 7.7-8.3 7.3 8.2 7.59 - 8.25 7.59 - 8.17 174 225 66 68 78 82 <0.10 <0.10 Intake 25.0 24.6 7.9 .3 7.9 7.86 7.80 189 67 79 < 0.10 ____________24.6 - 25.3 24.4 - 24.9 7.7 8.3 7.5 8.2 7.61-8.27 7.58 8.15 173-223 66 68 78 80 <0.10 - <0.10 Overall temperature CC) Pirnephales pronielas Cerfodaphnia dubia Average 24.8 24.8 Minimum 24.3 24.3 Maximum 25.4 25.3 9

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Piniephales promelas Test, Sequoyalh Nuclear Plant Effluent (SQN), UV Treated Outfall 101, February 8-15, 2005. Test Sample ID TCmperaturcC) DissolvedOxygen(mgfL) l p L(S.U.) Conductane Initial Final Initial Final Initial Final l mhos/cm) Control 25.0 24.5 7.9 7.5 7.85 7.58 296 24.8 - 25.2 24.4 - 24.6 7.8 - 8.3 7.2 - 7.7 7.70 - 8.20 7.40 - 7.79 278 - 341 10.98° 25.1 24.5 8.0 7.5 7.85 7.57 288 1 8 24.8 - 25.4 24.3 - 24.7 7.8 - 8.4 7.1 7.8 7.68 - 8.20 7.41 - 7.79 272 - 326 25.2 24.5 8.0 7.4 7.85 7.58 278 22.0% 25.2 24.3 24.9 25.3 24.3 24.7 7.8 8.5 7.1 7.6 7.69 I 8.20 7.39 7.82 265 306 43.9% 25.1 24.5 8.0 7.5 7.86 7.56 252 24.9 - 25.4 24.1 - 24.8 7.8 - 8.5 7.1 7.7 7.68 - 8.21 7.38 - 7.80 237 - 289 72 0% 25.1 24.5 8.0 7.5 7.85 7.63 219 24.7 - 25.4 24.3 - 24.8 7.8 - 8.5 7.2 - 7.8 7.67 - 8.21 7.47 - 7.84 205 - 255 40.%25.2 24.5 8.0 7.5 7.85 7.61 188 100.0% 24.9 - 25.5 24.3 - 24.8 7.7 8.3 7.2 - 7.8 7.66 - 8.21 7.45 - 7.84 170 - 211 Intake 25.1 24.5 8.1 7.6 7.86 7.60 188 25.0 - 25.3 24.3 - 24.8 7.9 - 8.3 7.3 8.1 7.66 - 8.21 7.39 - 7.88 174 - 215 Overall temperature CC) Pimephales promelas Average 24.8 Minimum 24.1 Maximum 25.5 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected February 6-11, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples was not sufficient to jeopardize statistical validity, although survival was improved in UV treated samples. 11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360.1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8. Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by EPA Method 330.5.

OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KC1 or NaCl.

2.

Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephales promelas. Sodium Chloride (NaCI crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. 13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes EPA/600/4-79/020 (March 1983).

4. Oualitv Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring February 8 - 15, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 11, 2005 2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 11, 2005 3

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 11, 2005 PCL-401 mg/L Copolymier 4

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations

of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - February II, 2005 5

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - February 11, 2005 6

Sequoyah Nuclear Plant Biomonitoring February 8 - 15, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_3 Clicnt: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyalt NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: NIA Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:

Carw NPDES Number TN0026450 Phone:

828-350-9364 044 c_ a o~dt c-Collected By: Wanda Allen Fax:- 828-350-9368 Field Identification/ Grab/Comp. Collection DatelTime Container Flow E 7' e 3; 89 Sample Description Nmber & (MGQ vnt ?taf,6a.,3y 3 e Volume ) (Mnrk as Appropriate) C ollected Date Time s Yes

IfrY, No Trace Tiy:

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NA SQN-INT-TOX Comp 2/06105-2107/)5 31 (2.5 gal) NA L 1 Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Wanda Allen 02107no0s Express Courier 02/07(2005 Express Couri 02/07/2005 FITS7 0210712005 __S____________r:. fS57 .QJbS~~~ 15 Instructions: Clients should fill in all areas except those in the "Laboratory Use' block. Biomonitoring samples are preserved by storing them at 6*C and shipping them in ice. Ile hold time for each sample is 36 hours from the time of collection. Therelore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time nrame. Samples shipped overnight on lriday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Enivironmental Testing Solutions, Inc. apa 410 Whole Effluent Toxicity Sample Receipt Log Uste Mm RetlvedI Remeved smpit Project Sple

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-d description St-Oe C-ment-reelv d by fram temperature (C) number number

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1683 050207.01 W %yuvlh WWIP TN 02*17U5 1350

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1. Smner Express Comner 1.3 1684 050207.03 TVA t

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BIOMONITORING CHAIN OF CUSTODY RECORD Pa ge _2_ of _3_ U Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Wanda Allen Environmental Testing Solution, Inc. 351 Depot Street. Asheville, NC 28801 Phone: 828-350-9364 FedEx UPS Bus Delivered By (Circle One): Other (specify): Express Courier General Comments: 4 CAoiA"An oLo S 1J Ad beat Ad A Client Fax: 828-350-9368 I ,ruce I Relinquished By (Signature): Date/Time Received By (Signature): Date/time Wanda Allen 2/9/2005 Exp s' Cozier V 2/9/2005 Express Courier £ p ( 2/9/2005 I34 l ETS 29192005 A. I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testing Solutions, Inc. page LZ. Whole Effluent Toxicity Sample Receipt Log Date Tim Rarelyu Rectved Sample Project Sample Sispl sme and dffcrpptu. state b rmlvd rmi b from b b nture u mber smber 02-09-05 0707 K Keena Gryhound 03 1692 050209.01 Bell Uneaproes Smkft ArmO M P NC 02409-05 0931 KI K K nta Wiboa Ttckmig 0.7 1693 050209.02 Tries, Inc.. Johntno unty Regkonal WWIp NC 02-0905 0931 KI Keatia WilonTrcking 0.7 1694 030209.03 tst. Ioc. South Cry WWP NC 0249-03 0931 K. Kean Wilson Thicking 0.7 1695 050209.04 TIest, Inc.

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NC 02-09 05 0943 K. Keema Fed - ex 03 1698 030209.07 Duke Energy at. - Marall Steam Stat NC 0209-03 1012 K. Keenan UPS 04 1699 050209.08 iiest, Ine.. WWTP =NC 02.09.05 1012 K. Keemn UPS 04 1700 030209.09 TlheKt Ine. Dynes NC 0209-03 1012 K. Keatan UPS 0.4 1701 050209.10 Tries% Mm - E1Ire WWI? NC 02.09.05 1012 K. Keenan UPS 05 1702 0302D9.11 Oxfoed Labnnuties, Inc.

Bdenbor WWTP NC 02-09.0 1012 K. Keen,.

UPS 05 1703 0os209.12 ox rord lAboi ,In Enield WWTP NC 024s090 1012 K. Kean UPS 0 5 1704 050209.13 Oxford Lxbone e. Inc.. Scothnd NeckWWP NC 0209503 1012 K. Kema UPS 035 1705 050209.14 Oxftod Aboltone, WIne. BASP NC 02.09.05 1012 K. Keenan UPS 0.5 1276 050209.15 Oxbrd IAboat Ie.. MoreheadCty WWIp NC 0240945 1012 K. Keman UPS 0.6 1707 050209.16 Mcobac nvrcamutal Labontory -Rockltin , WWrp NC 02.0943 1012 K. Kuona UPS 06 1708 050209.17 Micsbee flEieUnental I.AboFstoryDtero wWTPW_ NC 0240945 1012 KI Keeamn UPS 0.6 1709 050209.18 Mauobmc rn kWm aatulIAbony.J. P. Stevmns

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Client: TVA Environmental Testing Solution, Inc. 351 Depot Street Delivered By (Circle One): Project Name: Sequoyah NP Toxicity FedEx UPS Bus Client P.O. Number: N/A Ashville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: "eaj6 JA4A A~A~ +>2-- c50 4 CoodLL '.) NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Wanda Allen Fax: 828-350-9368 Field Identification I Grab/Comp Sample Description J-101-1 Express Courier Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testing Solutions, Inc. Whole Efmuent Toxicity Sample Receipt Log Ddte STb. R4 Relsed Sample Freje -P S e ample mam and dulptmstak iwibef I eale by from_ _ _ intde-v fa CC) number sammber 4 -w 7 02-11-05 123. K Kenan Hilrs speedy Dey 1.5 1683 050211.01 WayuesvI WWI?. Pp NC 02-11-05 1238 IL Kenan Mlrs Speedy Dtbtvy 1._ 1689 050211.02 Wm aymiiikeWWlp-Cd NC 02-1145 1313 J. Suneg Exprs" Cowiur 3.43.1 1614 050211.03 IVA-Smayvh Nucleahr Plnt-IOa 101 02-11.05 1313 J. Suner Exprs coe 3 0.1 1684 050211.04 IVA. Swpwyu Nuckw PFiN -Intake IN 02.11N03 133133 K. KIan Adhwvik cow= 0.3 1690 050211.05 CownscoleInC NC 02-11-01

Environmental Testint! Solutions. Inc. Page I of 6 Eniomna Tetn Souios _nc Pae1 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #: l1o89 County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 10.98 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: Organism age: .1S no V4.16 -t5 COLb Randomizing template: A42tU3 Date and times organisms 0eji-OS eto ro "O Incubator number. were bom between: Organism source: ASS ATncA 01-dl-CS Artemia lot number Transfer bowl information: pH = Temperature = °C Total drying time: 1 6. 2'I.3 Date /Time in: cl-tS-ofS IS Average transfer volume: Date / Time out: 62-tV6S 1&42 C Oven temperature: (1.C. Dailyfeeding and renewal information: Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time tMS O2.btOC6S O°3C l 13'Z a-- 0S A.22t.23 .lt I Oz-itaO d __530 IOA GOL -cs (OZ. 2 co2. I__ ______s C BPic ts C 02o6.-i6c~ Motqvu.+O'3+cq 3 vs6c -tS OIC I3 12 8 °-c-tS 0%3 q 2! t l.7-+. z3 KE 5 02-13-OS 0'30 tSz In4 Cr-1O-COS B 21Z1l. 03+.Oq KEI 6 (ML lz __S t3oZ. oz-zi x.ait.o4 KEV. 7C.4 Control information: Acceptance criteria Summary of test endpoints: %Mortality: 07O I s 20% 7-day LCso > IO et Average weight per initial larvae:

6. 6.l NOEC 1___I 0____

Average weight r surviving larvae: I. 4 2Ž0.25 mglarvae LOEC Il ChV 71 tm*7. IC25 I >10O,1lo

Environmental Testina Solutions, Inc. Page 2 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 02-o0 Q5 Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I I J K L /0/6 ° c'c 10 10 ID 0to 1I ,0 0 /0 2 1 IC)101 //0 t W o IC I_ l0 2 t/ /0 / 0 1/0 ID 1/ 0I /0 IL /0 3r i b 1o JO 10 IC) IO 10/0 / 0 .4 l) 10 o Io & /I IC) /0 ) 10 01 I D ( _ IC) IC 1 10 lO /to 1 10 1°- 6 /0 I t 10 el /0 ioqIo 4 d_ 710 -D /- -q to. ID 0 / IL ri q D1o q IC)~ A-Panweight(mg) Foae l_ Colorlden tldetl aIon:.. A jl V I u 1. J.73 ) 7 P1.S3 IS3 t 10 7 iL5i I1q.63 i,. Analyst: - I "1M 10.1 2] B - Pan + Larvae welght Analyst: T X Larvae weight (mg) - AB

1. °

\\S \\o >e U U 8 N Weight per Initial number A A. of larvae (mg) N k0 0 "C/Initial number of larvae j O O' O-OO Average Percent weight per reduction ..7 t Initial from control 0.(di'. 0 (6i5 O.0 01 number or (%). Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. k Calculations and data reviewed.

Vsnvirnnmental Testing Solutions. Inc. Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 0oz o0S Survival and Growth Data Day 43.9% 72% 100% M N 0 P Q R S T U V W X 0 lb 10 Io IC ) O 1 0 10 1 /0 10 10 10 1 10 10 10 O IC) 13 10 JO 16 JO 10 10 2 1 O I / lO i 10 _o - I) I 0 10 I10I /00 _10 C) I to 10 to to01 10 tO 0 I0 Jo 1 O ~10 10 10 10 100 10 8*o q'6 £0 1

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~ o ~ V Average PPercent weight per reduction .Aol Initial p from control .) 'O.I 'S.'s .1l, number of (% larvae (mg) Comment codes: c = clear, d = dead, fg fungus, k = killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Page 4 of 6 I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: 02-o8-0VS Survival and Growth Data Day = 100% Intake Y Z AA BB 0 )10 J 10 /0 1 /0 _ /i 10 2 I C/ J0 lo 13C 10 I I0 4 1 10

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( 0 1 /0 _0 10 /0 I0 A-Pan welght (mg) ?, fit Coloridentification: Bkit R-M l ijel 145s I4.59 Analyst: B - Pan + Larvae welght (mg) OV £2 4 Analyst: d °

4.

w* V Larvae weight (mg) - A - B Weight per Initial number of lanae (mg) 0 Cqo -C / Initial number of larvae O 0* O O Average Percent weight per reduction Initial from control O number of (%) lanvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: 4 Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-O2-013, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses aiest TVA) Test dates: Febn Project numben 1684 I S "oIyt Nadear Plant, OuthlU 101 Komatretsed arSy 8-15, 2005 Rnlwed by.

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00.0 0.649 63

-0.6 0 10 10 84.54 20.94 6.40 0.640 0.640 r 10 10 14.67 20.93 6.26 0.626 0.626 Q 10 10 14.68 21.16 7.1S 0.718 0.718, 72% S 10 9 14.63 20.49 516 0.651 0.675 4.7 0.95 80.0 0.545 44.0 1S.5 S 10 3 14.65 16.60 1.95 1 0.650 0.195 T 10 10 14.63 21.44 6.81 0.681 0.68l U 10 9 14 65 20.46 5.81 0.646 0.58l 200% V 10 8 14.49 18.87 4.38 0.54S 0.614 8.5 0.438 92.S 0.570 16.7 .11.6 W 10 10 14.64 20.62 5.98 0.598 0.598 X 10 10 14.60 21.24 6.64 0.664 0.664 Y 10 10 14.61 21.06 6.4S 0.645 0.645 100%htake Z 10 9 14.66 20.56 5.90 0.656 0.665 4.2 0.590 97.5 0.649 73 0.5 AA 10 10 14.59 21.64 7.06 0.76 0.706 BIB 10 10 14.59 21.3 6.54 -0.654 _0.654 Outfall 101: Duanett's MSD value: PMSD: Intake: Duanett's MSD value PMsD: 0.1898 29.4 0.0591 9.2 MSD - mifrum Sigificant Difference PMSD - Percent MianuSigtificant Difference PMD is enasu of test preciion. e PMSD tbh rnhm'mprent diffrence bween the control and tatn t that c hb declard ttitcay siificuat ima whole eluent toxity test. On yeragN, a s*ipficant difference occurs forEnvironmtal Testing Solutions, Inc. chronic toxicity tests when a toxicant reduce Pimcphatea gowtaby 15.7% from the control (deteidned tbrougd reference toxicant testing. wr MSD boad detrmned by USPA (I Oth pecnte) - 9.4% UpperPMSDlbound detemrnedbyUSEPA(90th percetile)- 35%,. The lower and upper bouadi were calculated by the USBPA using 205 tesb conducted from 19 laboratories for Pimcphates V)owth in chronic reference toxirant tests. USHPA. 2000. Underandingnd AccountingforMethod Vwiability in Whole Eluet Toicity Application, UndertheNational Pollutt Discharg Elirinatlon Proga EPA-833.R-00-003. US Enviros taProtecton Agency, Cincinnti. 01

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Larval Fh Growth and Smy Cvart-7 DayGrow Stbit Dait. 2ta Test ID ?pFRCR

SmptlD:

IVAIsueqpynhNudr plant - Outfill I01 E Date: 2/1Ys0 LabM. METSv. Testkig Sol Smple Toe: DMR-DMankMkgo popt Sample Date: Piot FWCE-R-PA421-R-02-013 Teat Specid: PP.Pnzpbaks pomekff Camzn: Non-tretd Came-% 1 2 3 4 r3-ankl .6460 060 0.6310 06970 10.98 0.6200 0.6160 0.6160 0.6920 22 0.61W0 0.6530 0.740 0.660 43.9 0.7090 0.6210 .6409 06260 72 0.7180 0.1S60 0.19so Qa6lo OD 0.5810 0.4380 0.59S0 06640 Tnnuteram thatrnalarned Rkt I-Tined Us0lmir Neta-% Mean N-Man Mean Mh Mes CV% N Sum Odni Man N-Men D-Jadol Q6453 .ooD 0.6453 6070 0.6970 5.J91 4 Q6565 LODDD 1os9 0.653s 1.0121 0.6535 0.6160 0.6920 6.259 4 18.00 10.00 0.6565 8.0000 22 06709 1.0395 6705 Q61t0 0.74J0 8t126 4 21.00 80.00 0.6565 LOOOD 43.9 0.6490 1.008 0.6490 0.6210 0.7090 62 4 1.00 10.00 0.6490 0.0886 72 05450 0.446 0.5450 0.1950 07o 44.012 4 17.00 10.00 OsJ76 0.1494 100 0 3 0.8Q33 0703 0.4350 Q6640 16.6J7 4 13.00 10.O 0.5576 .1494 Aasillary Test Statistc QhCald Skew Kourt Spko-Wik' Teadtkdkatwnan~nm d u txn (p <.O01) 0.837063551 314 -[73553777 6.57247M03 BDtletra Teat kidicate Ufeqivarcsmw e (p - 4.835-03) 16.33161411 15.0627224 ItypetheI Tot (IaUlH, 6.85) NOWC LOEC QV TU Steers Many-ODe RankTtSt 100 >10D I Tr7utrent; va DContl Limear Intarpolaltle (208 Reamphlo) Pait SD 95% CElgp) Skew 1cos IC'S 1d2D ICzs 1C40 ICSO 51.638 61.713 71.377 >100 >100 >100 >100 1.0 - 03 0.h - 0.7 - 0.6 0.5 i O. 02 0.1: 0.0 4 -0.1 0 20 40 60 to 100 12o Done % Does-RopousaFlot A I0.8-, 0.7 0.6 0.5 0 04 C0.3 0. 0.1 I a Oh P sqn_02-08-5dafa

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Larval Fblb Crvwt and Sarvtval Tut-7 Day Survival Sbtl Datc 2200 Test ID, YpFRCR Stzpk ID TVAW Seq3oyah Nuckar Plt - Outhfl 101 ]nd Date: VlSi20DS Lab tD IEava.Testg Sol SampleType: DMR-D dmarge M lgReport Sampe Date: Phocok FWPVR-EPA-821-R.02-o13 Tcst Species PP-Pnepbakea promelu Commentr. Nazn-rated Come.m 1

2.

3 4 D-uCto.l 1.0000 LOO LODOD 0.o 1092 1.0000 0900 1.0000 1.0000 22 0.9000 LO000 1.0000 1.0X0 439 1.0000 1.0000 1.0000 1oo 72 1.0000 0.9000 03000 iLOO 100 0.9000 QOOO1 LOD1 o LODD Traaiam Artsin Square Retd Rak I-TauL Number TuOu C0u.% Mea N-Mes Mes Mhs Mal CV% N Son Chkdcl Rasp Number D-Cono 0exo l.OOX 14120. IA120 I4L20 0.00o 4 0 40 10.92 0.9750 0750 13713 1.2490 14120 5.942 4 16.00 10.00 I 40 22 s7s50 0.9750 13713 12490 14120 5.942 4 16.00 laoD 1 40 43.9 LODOD 1.0LX IA1120 120 IA120 0.eoo 4 11.O 10DO 0 40 72 Q000 0.X000 1.1632 03796 IA120 34.091 4 14.00 10J00 40 100 0.9250 0.9250 1.2951 1.1071 1.4120 11.347 4 14.00 30.00 3 40 Aurillry Tosts Sbtab& Critcal skew Kuert Sbapro

sWk, Test Jiud s" m m al dietr m (p c-0ol) 0.772ss6395 0.84 2.08092234 7.173614905 Eqoaiy ofvwrseeamo be cmf~med eib Tat (1 allt 2s)

NOFC LOt C CtV TU Steel ManyO RaPink Tt 100 >100 I Tesbants vs D-Control Maibasum L eIlh..d-lr bkt Pazumetr Vahe SE 95%PldadalLbults C-eurl CrCht Oddnal Pvalue Me Sigm Iter Sbopc 1.069729589 OS54754S996 -1.62754953 3.7670084 0 9.020647Z5 7.tl47277B3 3.01-02 3.084281048 0934B156S7 3 RIaroept 1.70064959 1A8376844S.3.02136136 6.42265S279 TSCR 1.0 1.430t Prbi& 95%VldudcilIhnit 0ts ECOI 2.674 t.120492172 ECW 3.355 35.20901461 0°/ ECIO 3.71t 7696164709 / ECIS 3.964 13QU41172/ EC20 4.158 19S3933192 p 0.t ECD2 4.326 284.2924 EC40 4.747 703.51094C4 EC50 5.000 1214.196179 & 0.4 EC60 5.253 2094.699624 0.3/ EC75 5.674 5185.76039 EtO 5.S42 7431.040152 0.2 ECS5 6.036 11302Q245 0 1 EmO 6.22 191559292 0.0: EC95 6.645 41MOO870121 11o 100000 iS.06 EC99 7.326 Itl549.7565 100D S S4kin-h ctgsedtydetctd (p-3. .02) Daes-Rasposs* Flet 1x 4 fi 1I 0.9 0.8 0.7 0.6 en0.5 A0.4 r-. 0.3 0.2 0.1 04.I ft 8 ,O2-O-05damt

TVA / Sequoyah Nuclear Plant, Outfall 101 TVA / Sequoyah Nuclear Plant, Outfall I101 Non-treated Statistical Analyses Used for PMSD cajcuhlaUoa only. Larval Fih Growth and Survival Test-7 Day Growth St Date: 2/V2005 Tet ID: PpFrRCR Sazple ID: TVA/ ScqoaohNuclewarPJMl.Crifall 201 End Date: 2/152005 LAb ID: ETS-Envir. Testing SOL Sanple Type: DMR-DisdigeMcnit-ingRepcxt Sample Date: Protood: FWCiR.EPA421-R-02.013 Tea Speides: FP-Pimin es prmnelas Caimints: Noi-breated Cone-% 1 2 3 4 D-Cantmol 0.6460 0.6070 0.6310 0.6970 10.98 0.6200 0.6160 0.6S60 0.6920 22 0.6180 0.6530 0.7480 0.6640 43.9 0.7090 0.6210 0.6400 0.6260 72 0.7180 0.5860 0.1950 0.6810 100 0.5810 0.4380 0.5980 0.6640 Tunsona: Untnnsfonned I-Taied Coe-C% Mean N-Mean Mea MIN Max CV% N t-Stat Critical MSD D-Ctrol 0.6453 1.0000 0.6453 0.6070 0.6970 5.898 4 10.98 0.6535 1.0128 0.6535 0.6160 0.6920 6.289 4 -0.105 Z410 0.1898 22 0.6708 1.0395 0.6708 0.6180 0.7480 8.216 4 -0.324 2.410 0.1898 43.9 0.6490 1.0058 0.6490 0.6210 0.7090 6.287 4 .0.048 2410 0.1898 72 0.5450 0.8446 0.5450 01950 0.7180 44.012 4 1.2 2410 0.1898 100 0.5703 0.8838 0.5703 0.4380 0.6640 16.687 4 0.952 2410 0.1898 AislliaryTests Statistic Critical Skew Kurt Sblito-WHIkf Test indicates nvmmnaldistrituim (p -0.01) 0.3o6355 0.884 J1.78553777 6.5724703 Bartletts Test Indicates unequal vatimices (p - 4 g3E-3) 16.8316S41 15.0862722 Hypothess Test (I1-tail, 0.05) NOEC LOEC CkV TU MSDU MSDp MSB MSE F-Prob df Dunnetts Test 100 >100 1 0.18980068 0.29415061 0.01059594 0.01240485 0.52993709 5,18 Treatments vs D-Catlz s.qnfi248-OS5data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Larval Fish Grewthsad S ul.W Tavel DayqGrwtC Sltt Dafe: 281/200 Test ID PpFCR. Sampk ID: 7VA/ SequoyahNuck& Pant - Intake Bad Date: 2/152005 Lab Mr K-EBvk. Teatbg Sol Sampl Tpe: DMRDxcha geMumRms tepod SAmpk Date: P omtl FWCHRBPA421-R42013 Teat Spede: P?-Pknephake piumela Comments: Non-teated Caue. 1 2 3 4 D-ol 0.6460 0.607 0.6310 0.6970 100 0.6450 0.5900 Q7060 0.6540 Tnaazter Uatrazst'Iud 1.T e -T d lasWsAi CaaY% Mean N-MeaM Meaa bs MaS CV% N tstat Criica MSD lman NMesa D-Ostol 0.6453 1.00(o 0.6453 0.6070 0.6970 5.t9t 4 0.6470 J.OOX 100 0.648 1.0054 0.648I O.SX 0.7060 7.323 4 4.115 1943 0.0591 0.6470 1.000 AuxialmyTemb Statktic Olde Skew Kan Shapko-Wik' Teat kh dtea nmal dmtrgmtk (p > 0.01) 0.942065716 C 749 0.194721t2 .0.54745547 P-Teat hdkates aul variancea (p - 0.72) 1-551375001 47.46722794 Ify3pohesaI Tat (Ial.4& M.5) MD MSDp MSB MSE F-Pro& if Homacedasto t Teat kdcateta no maig ant diffaraces 0.059140771 0.091655593 lA4SS-0 0.001525U3 0.912197t88 1.6 Tremtments w D-Control Liearrlaterpeatia (200 RInamples) Pebt SD 95% CL(Zxp) Skew ICOS >100 ICIO >100 IC1s >100 1.0 IC20 >100 IC25 >100 1C40 >100 0.8 IC50 >100 0.7 0.6 0.8 0 4 0.3 0.2 0.1 0.0 -0.1 0 20 40 60 80 100 120 Dos % Dm.ipe-e p sPt 0.6.. 14al. 0.05 level of algriftcnnce 0.5 C0.4 0.3 02 0.1 8 sqpi 02-08-05data

Environmental Testing Solutions, Inc. Page I of 7 Eniomnaetn ouinIc aelf Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA . Facility: Sequovah Nuclear Plant NPDES #: TN 0026450. Project#: I A 6 4 County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%o) 10.98 22 43.9 72 100 Emuent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (nSL) 2500 2500 2500 2500 2500 Test organism information: Test information: t Organism age: 4~~A Zhoo I Randomizing template: Date and times organisms 20C a015 -M tWM4 Incubator number and Ct4? were born between: shelf location: Organism source: (l-o0- CS A - F YCT batch: 0- 2S Transfer bowl information: pH = f.1 Temperature = 2S.3C. Selenastrum batch: C2-03Ci5 Daily renewal information: Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0 o?-a -CS j3e 0-2-06-Os Q5D'C aOZ iLc-[ 2 L2-Ib-OS 10t.. o.2cfl-0os 002b

.2Zt*23 4 3

2-we-S _1030__ -4n-OS M 2.zty.z+.2? 4 ___-_2__ 10LO Il C-2 to-B s103 eD i_ q 5 a \\-Osls m OOS a0 Ow Z-0-J I3 05)1.03 +-4 d 6 -r0Iq 02-1dOs OG.121-.os

CYA Control information:

1 2 Acceptance criteria Summary of test endpoints: % of Male Adults: Ol 0% s 20°0h 7-day LC50 >160C % Adults having 3U1 Broods: 1C%3 co1- ? 80%NO NOEC Wf% % Mortality: .7l 0%7: s 20% LOEC > t0O% Mean Offspring/Female: 30; I Z.9 ( 2 15.0 offspringtfemale ChV I MOCZ. %CV: (..$Z % 6.t7 St% <40.0% IC25 >IOO%7o

Enivironmental Testing Solutions, Inc. Page 2of 7 Species: Ceriodaphnia dubia Client: Seguovah Nuclear Plant - Non-treated Date: i-09-bs CONTROL Survival and Reproduction Data [ ___Replicate number Da 1_l 2 3 j 4 j 5 6 7 8 9 10 Young produced 0 (Q C)0 I o_ 0 0 Adult mortality l 1 l l 2 2 Young produced [ J 0 I C g l 0 ° Q IC 0 Adult mortality L i t__ I 3 Young produced J i ) l J 0 C C 0 Adult mortAlity A zI-- 4 Young produced s Z l 5 S Is _ S L 5 Young produced ICQ 9 IC O 9 t 10II0111 1 0 Adult mortality l-- t-6 Young produced l O) o 10 O OI 0 1 cO Adult mortallfy l t C l l l 1 'l 7 Young produced l-4 o 13l l l 1 1 1 l15 I to Total young produced 9 q 3 z 3 0 l l 3 .3 3 I 3 Final Adult Mortaity C. l I ll Xfor3'~ Broods ? I l;_ >4 lC l l C Note: Adult mortality (L -.live, D - dead) I Concentration: J % Mortality: l 0% Mean OffspringFemale: 3.1 CONC: 10.98% Survival and Reproduction Data I ReIpicate number I l D aY l_I_l 2 3 54 6 7 8 9 1 10 l I Young produced

Ql C0 Q

O l 0 l l l° 1 Adult mortality L-L - L-L- 2 lYoung produced l', C) C rol lc nI In l l ~~Adult mortality l I _lLlLl lllLl-l 3 Young produced l [ Cl 2 I Adult mortality l C-

l l4

.lYoung prodaced l 9l l5l5 = L tS Adult 1 1 L s Young produced 10l I l 12I liAdul 1 t I l IC) I b I O a Adult mortality l-C. l L-.l LT.-l 6 Youngproduced 2l. 1 I b l_ C) l ll Adult mortality l-l l C-L- V 2l +/- _ 7 ILI 1 7 l Young produced 11° l 1 l I ll I 0 l Total young produced l 3 3 l-Zl 3 Ib l 3Z l 3 2l-! Final Adult Mortality I L l l l L-l- i l - l i Cqe t Note: Adult mortality (L - live, D - dead) Concentration: % Mortality: l A Mean Offspring/Female: I31.1 % Reduction from Control: -S.3%

rnvironmental Testing Solutions. Inc. Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: pi-o&-as CONC: 22% SurvivalandReproduction Data Replicate number Day 1 2 l 3 4 6 7 8 9 I 10 1 Young produced O O. O C l t l) *Q O Adult mortL L t Ly 2 Young produced O Q _. l o l O O Adult mortality [ L l L4 t i 4 L C? 3 Young produced oiC O C O lI lc c C Adult mortality 2Ll_I 4 Young produced Is [L A S I S S l IS Adult mortality L-L- L-. U L a 5 Youngproduced I l L3 l Il t 2-I t. l lC so et l 1 ° Adultmort lity Ll L= L a 6 Young produced o 1 Adult mortality L-l U. L L-f U ¶ l t 7 Youngproduced I e Z: l 8 i l ILA Total young produced [3Z 3 S 35 3 L32. l32 81 3 T T2 Final Adult Mortality C l l I I l r Note: Adult mortality (L - live, D - dead) Concentration: l Mortality. 0% Mean Offspring/Female: I 3z.Cj % Reduction from Control: .31 CONC: 43.9% Survival and Reproduction Data Replicate number Day 1 1 2 3 4 5 6 7 1 8 9 10 I Young produced C0 0.. l lO l C Q l_ O0 I l Adult mortality U. U. r 1-l U l 2 Young produced C) lO O O l Adult mortality (.. (-l I-_ l U l 3 IYoung produced a 1: I a SN Adult mortality 4 Young produced l__ I l lAdult mortality l k LlLl-l 1-l l1 Ll 5 Young produced 1ll3 l1 l C) lI l3 to I O Adult mortality l-X-L-% T-l I_ l--I lll 6 Young produced la 8l C) cl O l 1-1 lO. Adult mortality l l Ljl L l l 7 6 Young produced I JI1-II 010 ) 0I1I.Q ..... oung produced S 1 o I-a' 20 i-o I. T 3otalyoung3produced 1 b l4 l 3t. l I 4 l 5 b 1 31 Final Adult Mortality l -7 LU l

l.

I U

l.

I Note: Adult mortality (L - live, D - dead) 6AtZOb Concentration: % Mortality: 07' Mean OffspringfFemale: 3 1. 5 l 'V. Reduction from Control: - IL'U 1

rEnvironmental Teting Solutions, Inc. Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated .e->ii -

nrc 72%/

Surv Page 4 of 7 Date: oz-ot oS ival and Reproduction Data 4 IRepicate-number Dy l 1 2 3 4 l 5 6 l 7 l 9 10 o1 ung prm 01 0 O O o Adult mortality U L L U U U U U ,.2 Youn p (duced_ O Adultmorlity l = l L 3 Young produced l C) ° C Cj I C I C) IQ\\ CO Adult mortality l j L l l l l > 3 Young produced [* f 400 W 10 Q44

)

Adult mortality [ U U L U 5 Young produced t IC l.t3 I O 5 c III Adult mortality L L I L-.j L-6 Young produced C.)_o C) 0 ~~~Adult mortality l t .7 Young produced l6 0 lO C) C) tS L O1 I 0 ql 6 Totalyoug produced lT Fina l Adult Mortality l L l l l U l ll.. Note: Adult mortality (L - live, D - dead) 7 lo Concentration: l Fin*l Adult M ly Mortality,l l Mean Offspring/Female: l 34.( l % Reduction from Control: - S.0% CONC: 100%. Survival and Reproduction Data l l__ RepIlcate number I Dy I1 2 3 4 l 6 l 7 l 8 l 9 I 10 l I Yon rdce )C 2 Young produced II I l l ~~Adult mortality ll _lt Adult mortality U-U 4 Young produced - A lll Adult mortality l l l U l U l U.tl L a .4 l 5 Young produced I 9l1 ,zTlb1lAlt111\\4l Adult mortality Lj 6 Young produced jo la it U C) I Adult mortality L4 !!! L-L t-5 Youngproduced l2. l 9 o I ° C) a z ,I zoI 121 6Total y oung produced 1I ii 0 3 3k 23 1 1 i7 I Aut otiy Note: Adult mortality (L - live, D - dead) Concentration: I % Mortality: I Mean Offspring/Female: l 35. &8 % Reduction from Control: - Id 1

-Environmental Testing Solutions, Inc. J-. Species: Ceriodaphnia dubia Client: Secluovah Nuclear Plant - Non-treated CONTROL Sun Page 5 of 7 Date: 02-o-OS 'Iva~l and Reproduction Data t ~Replicate number Day 1 2 3 4 1 5 6 7 a 9 10 I Young produced 0)( 0.C .D. ~~ .C.. L [ Adult mortality V-1 L.. ? L Young produced L A iL)i 0 Adl3mrait Young produced C)c. ~T~t Adult mortality 10 k-I ma

~

LI LA '4 I 4 Young produced ~LL $5 T C i -Adult mortality L L ~ ~ ~ J '.~ s Young produced I[ t 10 01 1 I10 I 0 I I Z. Adult mortality Lj - L-L- L-L- L. L L-. L 61 Young produced j t010 l O oc C 0O [Adult mortlity L-. '...] t -.. jL. 1.. 7 ~Youngproduced~ c I's I5 t I Ito~ 1'S* 12.. II b L Total young produced -- ~ Final Adult Mortali I L.. L. y -~ Note: Adult mortality (L live, D -dead)_ IConcentration: I% Mortality: 0 7 Mean Offsptnin emale: 2Ci.(e coNC: 100% Intake Survival and Reproduction Data [ ______Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced r) C ICX 0 j.Q L Q 0 Adult mortality L-L- %--U L-.. t.. 2 Young produced C)C )0 ~-r~ C_- Adult mortality L-L "I UU L-U 3 Young produced 0 )~ o o1C Co 1CI)~ Adult mortality L_ FIC '-K-- 4 Young produced 15 NI IS I4 -- S-L4W~ Adult mortality L-L?~ L -L-T I L-- L s Young produced 1J 2 r7 l1 ~1 -, I a 110 1 IC II 12. Adult mortality j U 1i T-6 Young produced I... I I ...O..I I O o o 10 Adult nortaity IL 1U ~ U LI .l UL 7 Young produced i6 160 1' S -7 i l I Total young produced S .72 3tA

4 I32z 33 32..

31 33 '5S Final Adult Moralty% I -= 7 I cA4/-'4 oi)e&C Note: Adult mortality (L-live, D - dead) Concentration: % Mortaty 7 Mean Offspring/Female: 'A7 % Reduction from Control:-;.7

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Verification of Cerdodaphnla Reproduction Totals Control-I DayR ,e num-ber 1 2 3 4 5 1 6

7.

a 9 l 10 TotS I O O O O -O a 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 -0 0 0 0 0 0 0 0 4 5 4 5 S S 6 5 5 4 4 48 S 10 10 9 10 9 11 10 10 11 10 I 100 6 0 0 0 0 0_ 0 0 a 0 0 0 7 14 16 13 15 14 17 17 16 15 16 153 Total IF 30 27 30 28 34 32 31 30 30 1 301 10.98% YR -e Tombertal 1 2 3 4 S 1 6 1 7 1 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 5 5 4 5 5 5 5 47 S 12 12 11 10 11 10 10 10 10 9 105 .6 I 1 0 14 16 17 -0 0 16 0 65 7 14 19 16 0 0 0 -19 19 0 13 100 Total 31 36 32 29 32 31 34 34 31 27 317 22% Day ~Replicate RumberToa D I 1 2 3 4 S 6 1 7 8 9 10 Totl 1 0 0 0 0 0 1 0 1 01 0 0 1 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 4 5 4 5 5 5 5 S 4 5 4 47 5 1 1 13 1 1 12 10 12 10 10 9 10 I 108 6 14 0 0 18 0 0 0 115 0 0 47 7 2 18 17 0 17 15 16 3 16 14 118 Total 32 35 33 3S 32 32 31 321 30l 28 320 43.9% Day Replicate nunber Total 1 2 1 3l 41 5 6 1 7 1 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 O 0 0 0 0 0 0 0 0 4 5 5 5 5 5 6 4 5 4 S5 49 5 1 12 12 13 12 10 11 13 10 10 14 117 6 0 0 18 00 0 0 0 171 0 35 7 15 19 0 17 19 20 17 19 0 1 18 144 Total 32 36 36 34 34 37 34 34 31 37 345 72% D R ep ternmber I Total 1 2 3 4 S 6 l 7 l 8 9 10 I 0 0 0 0 0 0 o 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 5 4 5 5 6 4 5 4 48 _5 12 12 11 11 10 13 12 10 13 1 111 115 6 0 20 0 1 6 17 0 0 0 18 L_20 I 91 7 16 0 20 0 0 18 19 19 0 0 92 T tal 33 37 36 31 32 36 1 371 33_ 36 35 346 100% a Day ReplUcate number Tdta y 1 2 3 4 S 6 7 8. 10 1 1 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 5 6 5 6 4 5 5 5 5 1 5 12 14 14 12 10 14 1 1 1 1 12 12 12n 6 17 0 0 171 0 18 20 0 0 0 72 7 0 17 19 0 190 0 21 20 17 113 lTota 35 35 38 35 l 34 l38 35 37 37 34 358 Control-2 D Replicate numnter IT 1 2 1 3 1 4 S 6 1 7 1 8 9 10 I 0 0 1 0 .0 0 0 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 00 0 0 0 0 0 4 5 4 5 3 4 5 4 4 4 S 43 5 10 9 10 10 9 10 10 10 12 10 100 6 16 0 1 0 0 0 0 0 0 17

7.

0 15 15 16 16 15 12 17 16 14 136 Total 31 28 30 30 29 30 26 31 32 29 296 100% Intake I Replcate nunter Total Day 1 2 3 1 4 5 6 1 7 l 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 S 4 4 4 5 4 5 4 5 5 45 5 10 12 1 1 12 12 10 1 10 11 121 113 6 0 0 0 0 0 0 0 0 0 0 0 7 16 16 19119 1 17 18 1 Tot 31 32 23 3 33 32 31 33 1 35 327

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Cerlodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA/Sequoyah Nuclear Plant, Outrall 101-Non-treated Client: Test dates: February 8-15, 2005 Project number: 1684 ,1 -I Revdeled by:- I Concentration Rep _licate number l_Survival Average reproduction Coeffident or Percent reduction from (%/) 2 3 4 5 6 7 8 9 10 (%) (oflspringlfemale) variaton (/.) pooledcontrols (%) Control -I 29 30 27 30 28 34 32 31 30 30 100 30.1 6.5 Not appilcable 10.98% 31 36 32 29 32 31 34 34 31 27 100 31.7 8.2 -533 22% 32 35 33 35 32 32 31 32 30 28 100. 32.0 6.6 -6.3 43.9% 32 36 36 34 34 37 34 34 31 37 100 34.5 5.8 -14.6 72% 33 37 36 31 32 36 37 33 36 35 100 34.6 6.3 -15.0 100% 35 35 38 35 34 38 35 37 37 34 100 35.8 4.3 -18.9 Control - 2 .31 28 30 30 29 30 26 31 32 29 100 29.6 5.8 Not applicable 100% Intake 31 32 34 34 32 33 32 31 33 35 100 32.7 4.1 .10.5 Outfall 101: Dunnett's MSD value: PMSD: Intake: Dunnett's MSD value: PMSD: 2.136 7.1 MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Cerfodaphnla reproduction by 9.7%/ from the controL Lower PMSD bound determined by USEPA (10' percentile) - 11%. Upper PMSD bound determined by USEPA (90' percentile) - 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. 1.192 4.0 USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under Ihe National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Oniedapkala S rvivel and Repruartou Test-lRepadverd Start Date: 2tV2005 TealDM. CdPRCR Sample ID IVAI/ Scqoyah Nuclear Plant - 02 k 101 EndDate 2152005 Lab MD. -Evi. Teatg SOL sample Type DMR-Dkcba MkrgrgReapt Sample Datc Protocol FWCHR.EPA-121-R.013 Ted Spe CD riDpnk dubi-rbenhn: Non-tated Cac-% 1 2 3 A S 7 a 9 1i D-CCIMo 29.000 30.000 27.000 30.000 28.000 34.000 32000 31.000 3000 3Q00D 10.9 31.000 36.000 32.000 29.000 32.000 300 34.C00 34.000 31.000 27.000 22 32.000 35.000 33.000 35.000 32.000 32.000 31.000 32.000 30.000 2t.000 43.9 3Z000 36.W 36.000 34.000 34.000 37.000 34.000 34.000 31.000 37.000 72 33.000 37.000 36.000 31.000 32.000 36.000 3.000 33.000 36.000 35.000 100 35.000 35.000 33.000 35.000 34.000 3:.000 35.000 37.OOD 37.000 34.000 Trsasurw. Uutruansard I-Tana h meulk Cae. Mug

N4-Mm Maem Mia M"

CV% N Utat Critic MSD Mms N4Meam. D lCofrol 30.100 IJ000 30.100 27.000 34.000 6.542 10 33.117 1.00 10.98 31.700 1.0532 31.700 27.000 36.000 8.152 10 .1.713 2.2S7 2.136 33.117 L0000 22 31000 1.0631 32000 2t.000 35.000 6.511 10 -2.04 2.217 2.136 33.117 1.0000 43.9 34.500 1.1462 34500 31.000 37.000 5.337 10

4.711 2.217 2.136 33.117 1.0000 72 34.600 1.1495 34.600 31.000 37.000 6.273 10

.4.111 2217 2.136 33.117 1.000 100 35.t00 1.1394 33.100 34.000 31.000 4327 10 .6.103 2.2t7 2.136 33.117 1.000

tia C

Srti ti>al Skew Kurt Kohnoov D Test bdkates nomal dambutin (p > 01) 0.65310415 1.035 -0.12175496 -034410075 Batewt Teat indicates equat van e - 0.11) 2.2S7753t2 15.0t627224 Itylaln ub Tut (1.&H, 0.85) NOWC LOEC aV TU MSDa AM P MSB M EF F-prah dt Duometr Test 100 >100 1 2.135582S67 0.07049597 4733666667 4361111111 2.9E-07 5.54 nens t vs D-Ceairol L.mearlserpuludua (20 Resp e.s) Palat SD 95%CL skew 3C05 >1oo IC1O >100 ICIS >100 1.0 IC20 >100 0 i IC25 >100 0.8 IC40 >100 0.7 ICSO >300 0.s 0.4 &0.3 0.2 0.1 -0.2 .03 0 20 40 60 80 100 120 Doe % Due-Rmspwse riot 14all. 0.05 level oft alfcnce I xqt_02I08-05doia

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses CwledPka S sl.d ud Reprodacdow T~ent.tepreduecio Stut DO-,c s22= Test ID CdPRCR Sample It TVAI Sqwah Nuelar Flmt - Inake End Date: 2t5/2005 Lab ID. ElS-En*. Tatig Sol S-pk Type DMR-Dcharp M S Rspot S Wmp eDat: PFrocot FWCHR-EPA-t2-R-2-013 Test Speck: CDCcpbnk dubit Cametah Non-bDated Cmsa% 1 2 3 4 5 4 7 a 9 1i D-Centol 31.000 28.000 30.000 30.000 29.000 30.000 26.000 31.000 32.OD 29.000 100 31.000 32000 34.000 34000 32.000 33.000 32.00 3L.000 33.000 35.0OO TrRSfe.t U.Usterwd 1-Tteid IjOtck Came% Mum NMes Mes min Mat CV% N t Sb St Olikal MSD Mes NMesm D-Ctol 29.600 I.OCO( 29.600 26,000 32000 5.n76 10 31.150 1.0000 100 32.700 1.1047 32700 31.000 35.000 4.090 10

451l 1.734 1.192 31.150 1.0000 SUdAUbTUW St ic Qikal Skew Kirt Sbxpko-W&S Tot tdxca no sid t

(p> 0.01) 09600024 0.86S .43309141 0o313519723 F-Test hidltes equalvamx.e (p 047) 1.639751554 6541089S353 Ilyl~pas Ise Test (I-ftl~ IUS) uslla MSDp MSE MISE F-Preb dr HoCsscedast Test kbdmtes nwhimto d cffaca 1.191621241 0.040257475 43.0 2.351111111 2.7E-04 I1'l Tzubmcovs n D-Corol Laerar ItPWVetlem (200 Rtauampesa) peat V. SD75%t Sk ICO0 >100 ICIO >100 ICIS >100 1.0 IC20 >100 0.S IC2>100 0.8 IC40 >100 0.7 ICS0 >100 0°7 0.6

0.5 0.4 10.2 0.2 0.1 0.0 40.1.

0 20 40 So g0 100 120 DO" % mm. ss 15' 30 1411,0.05 leve 25 .o signlcarice 20 10 e xqn_°2-M0JSdafa

Environmental Testing Solutions, Inc. Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA Facility: Sequoyah Nuclear Plant NPDES #: TN 0026450 Project #: I loeA County: Hamilton Treatment: UV-treated Outfall: 101 Dilution prepar tion info mation: Comments: Dilution prep (,). 10.98 22 43.9 72 100 Each concentration was treated Efuent volume (mL) 274.5 550 1097.5 1800 2500 for 2 minutes with a UV sterilizer Diluent volume (mL) 2225.5 1950 1402.5 700 0 to remove pathogenic Total volumc (mnL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 2l.5 rb ,-lrtoj5 Gab Randomizing template: &Q Date and times organisms O}2-0-¶C5 MM -0 I bOO Incubator number: 56 were bom between: Organism source: i'6S BK-TA 02.-O1-os Artenia lot number: 6 S Transfer bowl information: pH = Temperature = °C Total drying time: _ 1.b 2.d Date/ Time in: o2zIoS Nt; Average transfer volume: Date / Time out: 0 tb-c0 1IZO Oven temperature: I t Dailyfeeding and renewal information: Day Date Morning Afternoon Test initiation, MHS Sample numbers Analyst reeding feeding time renewal, or batch used used time termination time 0 02-lf;O 1330 O-b06OS bWZD&+.024,'0o3 A i Czd-cRf CA0-5z vlS30 1cco OZ-06-6 OzOq'Oz.02h 2 r i cS Oct's-t t3l\\ n -o5-O2o0. ZZI,23-- 0?oz-l%-OS-ORI vSXt ISQ4 csl-n-O Mw2D. 2+Z 4 Caz-ts 0 zo I 153 B I?- ( zo-o 10 SS 60,2 1. o-:5 -hO )<EK s cz w-0S OqcV0 15353 1 z366 cs7 120 Oc6 UZ2.11.oM+.p' KEI 6 .t1q-S 1.zi 17 IZL4 oz.1'?zoS b j i +.3q g E I-t Control information: Acceptance criteria Summary of test endpoints: % Mortality: I0% s 20% 7-day LC50 > I 06-1b Average weight per initial larvae: . knS .- t-...'NOEC l bOQ% Average weight per surviving larvae:

0.

0Aq5 2 0.25 mg/larvae LOEC > I Ol. ChV IC25 1 > tool,

Vjnvironmental Testinv Solutions. Inc. Page 2 of 6 .I.nvrnmeta1Tetig oluios.In. Pge2 f Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: C0z-(A-LYS Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I J K L 10 (0 /0 I) /0 10 /0 /0 /0 /0 /6 10 1__1O/0K1 1I/0{ /0 /0 l'c /0OC /0 2 10 /t 10 /) /0 10 to lo 10 to 16 't 3 1_____ 0 10 /D 1(/ IC to ./o /0 10 10 4 1 b' Io 'o Ioi t __ lO IO 100 J o 1 o l 1t0 b 10 O 0 IC 10 Io 6 I 1 0 /010 10 10 10 10 I10I10 10 7 10 I 0 10 IIF L-lO 1 It to I 1 10 10 10 1o* I I I I I I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Thnvirnnmental Testing Solutions. Inc. Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: QC2-cR-C6 Survival and Growth Data Day 43.9% 72% 100% M N 0 P Q R S T U V W X

0.

1 Io IoID o/O ID /O 1/ / O10 2(0 /0 /0 I0 /0 6 1o 1 /0 O /0 1 10 o,o - Io I0 10 IO _O q C0 1 IC) l 0 /0 /C) IO l0 lo0 I0 lC0 9 /O /O 6 -1010 1/0 10 t 1(0I 0_ _ _ /0 6 1 0 /O l) l 6 t> I t) l0 I J 10 IC Q J()) l (

7.

-s I5 M /O 1 /0 I0o IC) to **-l 1 0 9 .o ,0 A-Panwelght(mg) 'at-)L Color identification: g qe.qO MiS 1i.71 _9 349 ,sl 15.oo 1q.6/ N4Mp/ q.-6s 1sx7 qsi is-eto Analyst: PC %Q s Vf2 ° f B -Pan + Larvae weight (mg) A 4J A Analyst:

A

\\ \\ Larvae weight (mg)-A - B A A A AA 'V Weight per Initial number 'V of larvae (mg)AA' Cll nitial number of larvae 'j,

4. 1" Average Percent weight per reduction o.

ttL4 O47% Initial from control -2.i LJ.,5 O.Is.17 number of (%) larvae (mg) 6 Comment codes: c - clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

TPdinv.1Rnlnflnnr.- Inc-Page 4 of 6 LILVAL ".===-- Species: Pimephalespromelas . Client: TVA i Sequoyah Nuclear Plant - UV-treated Date: oQ2O-0OS. Survival and Growth Data Day 100% Intake Y Z AA BB O /0 10 10 /O 3 to0 /D /0 2 ~ ~~~ 1 t () lb 10 to 6 t o /0 tL) to

10) lb 1/0 A - Pan weight (mg) Sloe Color Identification: 1k i.7 j

14.93 /'..J5 Analyst: fe....................... B - Pan + Larvac weight Analyst: ""O ,0o Larvae weight (mg) - A-B Weight per Initial number of larvae (mg) 41 t N C / Initlal number oFlarvae No 'a Average Percent weight per jreduction Initial from control O.( b S oio number of (M) larvae (mg) Comment codes: c clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. I Calculations and data reviewed: ) Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: PImephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client:

TVA, Test dats:

Ptebe Project numben 1684 i Seqoyal Nuclear Plant, Outfall 101 UV treated iury, 85,2005 Rteselwed by: Ar7 e 1 trft~bre -?A..dZg R.r§*L.MQ Lame *Htbo(. W.4gallS.rrM. M _l t radltr Wellb l-mml r M..rrlral Mr s-bt C _*tiw. P. .Aea

e

.me (-tl -MStb(-O -4.3 rrtbrr.n l(.w) Srrt'be-Gt a! bhmuwel OA) Iktda.brW6 witS.. r.- t (4) A 10 10 14.52 20.84 6.32 0.632 0.632 Contrtl a to O0 14.33 21.91 7.3t 0.738 0.695 6.5 0.738 i00.0 0.695 6.5 Not applIcable C 20 10 14.63 21.62 6.99 0.699 0.699 D 10 t 4.65 21.76 7.11 0.711 0.711 10 10 14.70 22.08 7.38 0.73J 0.73t 10.98% P 10 10 14.73 22.16 7.43 0.743 0.710 6.0 0.743

97.

0.694 10.7 0.8 a 10 10 14.51 21.61 7.10 0.7 to 0.710 88 10 9 14A9 2034 5.85 0.650 0.585 10 to 14.83 2142 6.59 0.659 0.659 22* 20 IO 15.07 21.54 6.47 0.647 0.676 4.0 0.647 100.0 0.676 4.0 2.7 2%K 10 to 14.85 21.83 6.98 0.698 .7 0.698 L t0 20 14.84 21.84 7.00 0.700 0.700 M O 10 14.90 21.17 6.27 0.627 0.627 N 10 10 15.45 22.51 7.06 0.706 0.676 53 0.706 too.o 0.676 53 2.7 0 t0 t0 24.77 21.75 6.98 0.698 0.698i P 10 0 4.94 21.67 6.73 0.673 0.673 0o to 14.51 20.28 5.77 0.577 0.577 72% R 10 10 15.00 21.92 6.92 0.692 0.615 9.3 0.692 100.0 0.615 93 t1.6 S I20 8 0 14.64 20.31 5.67 0.567 0.567 T 0 0 14.94 21.16 6.22 0.622 0.622 U 20 10 14.68 21.87 7.19 0.719 0.719 100% V I0 9 15.07 20.8Z 5.75 0.639 0.653 12.1 0.575 97.5 0.637 13.9 8.4 W 10 10 t4.89 20.36 5.47 0.547 0.547 X 10 10 15.10 22.16 7.06 0.706 0.706 Y 10 t0 14.78 21.12 6.34 0.634 0.634 800%- Intake Z 10 10 24.98 22.05 7.07 0.7607 0.660 5.8

0. 707 200.0 0.660 5.8 5.0 AA 10 20 84.83 221.07 6.24 O.6 0.7624 BB 2

toO1 14.95 21.70 6.75 0.675 0.675 Outfall 80t: Dunnett's MSD value: PMSD: Intake: Dun ett's MSD Value: PMSD: 0.099 144 0.0575 8.3 MSD-Minimu SignificantDifference PMSD - Percent Minimum Significant Difference PMSD i a measure oftest precisio The PMSD is the muiniums percent difference between the cotrol and treatient that can be deelared statistically significant In a whole effluent toxicit test. On average, a significant difference occurs for Environmental Testing Solution% Ic. chronic toxicity tests when a toxicant reduces Pimephates growth by 1 5.7% fotm the control (detesnined through reference toxlcant testing). Lower PMSD bound determined by USEPA ( Oth percentile) - 9.4%. Upper PMSID bound determined by USEPA (90th peren tile) - 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 89 laboratories roe Pimephuin growth in chronic reference touicant tests. USEPA. 2000. Understadig and Aceoating foe Metod Vaiablity In Wbol EfEluentToxicityApplctions Uder the National Pollatat Discharge Elimiation Pgm EPA-833-R0o003. US Envimtl Potecldon Agrcy.Clacinatd. OIL

TVA I Sequoyah Nuclear Plant, Outfall 101 TVA / Sequoyah Nuclear Plant, Outfall 101 UW-treated Statistical Analyses StaS Date: 24t=2CO Test ID: PPYRCR 3Smpie ID,. TVAI Scqtyah Nuckar PNoit-OuW101 En Da te 2f1152005 abM. MElEvir. Testog Sot SapvITypc DIO R Dl da MO-at lRepcet SampIe Date: Protocot FWCI-EPA-821-R-02.013 Test Speces: P.-piepbaks promelha Commei: W-treated CAe-% I

t 3

4 D-Coafrol 0.6320 0.7380 6990 0.7110 10.91 on73o 0.7430 A7100 0.150 22 0.6590 0.6470 Q6910 0.7000 433 0.6270 0.7060 0.6910 Q6730 72 0.o770 0.6920 0.5670 6220 100 0.7190 0.5750 05470 0.7060 Tnamsfrmu Uatmasformed I-Taited Iswtook Co.e.h Mea N-Mesm Mles Mis Mesi CV% N t-Stat t Cstkal MSD Mfan N-Mean D-Contni 0.6950 I.ODD 0.6950 0.6320 0.7310 6.4S3 4 0.6950 I.COOO 10.91 6940 0.9916 0.6940 0.5t50 0.7430 10.67n 4 0.024 2.410 0.099 0.6940 0.9936 22 0.6760 0.9727 0.6760 0.6470 0.7000 37 4 0.459 1410 0.0991 Q.6760 0.9727 43.9 0.6760 079m Q6760 Q6270 0.7060 5261 4 0.459 2.410 0.099S 0.6760 0.9727 72 0.6145 0t.U42 6145 05670 0.6920 92.65 4 I3944 2.410 0.0991 0.6256 0.900 100 0.636: 0.9162 0.6368 0.5470 0.7190 13.179 4 1.407 2.410 0.099S 0.6256 0.9002 AvulIhLyTots Statlde Crideal Skew lart Sbapto-W~s Teut ctes nomnl dattutr (9p> 0.01) 0966917574 0.t4 037651176 -0.53421699 gartletrz Test Indicates equal vui& = - 0.43) 4.57076645 15.0S627224 OyptehesI Tes t Q lal 0.05) NOEC LOEC OCVN TU MIDm MSDp MSE MSE FPro t D=)Wneu Test 100 >10 I Q099773349 Q1435335775 O.4264375 0.tC 27175 0.329913467 5.11 Trhatretb vs D-Ctrol_ LJmarlaterphstia (290 Resomples) Poist Y SD 9S% C4Exp) Skew IC05 52.6S6 ICIO >100 IC1s >100 1.0 IC20 >100 09 IC25 >100 IC40 >100 0.8 IC50 >100 0.7 0.06 CA 0.3 02 0.1 0.0_ 0 20 40 60 80 100 120 Do** % Dot.Rposse Ylot 0.7 0.6 I -tall. 0.05 levol dosignfcance 0.5 0.1 02 sqmO2-M085dala

TVA Sequoyah Nuclear Plant, Outfall 101 UV-treated Statistical Analyses Larml FisC. G n d SwrvivslTeat-7 DayGrwwt Start Dite: 2N2005 Tat lD: pFFCR ScmpoleD: 7VA/ Sequoyh Nucr Plant - labke End Dste f 120t 5 Lab D. MES vk. Tesftg Sol Sample 7lpt: DMR-Dkd-u Monlaig Rtport SampleDate: Pm3toco I PJWCR.-EA421-R.C2-013 TestSpecies: PP-P epbalesprumpmas Comments: WUvated os.e-% 1 2 3 4 D-Conml 0.6320 OL73B0 0.6990 0.7110 100 0.6340 Q7070 Q6240 Q6750 Trsas~f:r Unfrsastamred I-Tsied lsetask Come.% Mesa N-Mean, Mean Mis Ata CY% N Sto. Critical MSD M^es N-Menu D-Cowtol 0.6950 1.0000 0.6950 0.6320 0.73S0 6.A3 4 0.6950 1.0000 100 0.6600 0S9496 .660 0.6240 0.7070 5 t07 4 1.13 1.943 0.0575 0.6600 O.496 Anxilhez Tests Statistic__ OCde Skew Vurt S0.oW9etDlzethmzdboten >O2) 0343340774 0.749 .0.404S701 4Jlt646t7 FPTest hdicaten equialvarinnces (p - 0.Q0) 13t2206022 47.46722794 1y etea tllO.I) MSDs MSD9 MSB MsE F.Prob 4r H{ainoseedastirtTest Indrates no &*Mjfxstderefences 0.057469099 0.062689352 0.0245 0.001749333 0.211393141 1.6 Tratments vs D-Ce0sI Linear Isterpolation (200 Resamples) Point Y SD 95%CrL(xp) Skew 1C05 99.286 lCIO >100 ICIs >100 1.0 1C20 >100 o. IC25 >100 IC40 >100 0.8 ICS0 >100 0.7

kidcta IC atiat szanthtkywestcoce=ation

.06 ,X0.4 0.3 4 0.2 0.1 0.0 -.rrr, : 0 20 40 60 60 100 120 Doss % Doe-.Itap~saeFlat 076 0.74 0.72 0. 0.7.. t0.66_ t0.64 al..0.05 lcv 0.62 0.6 0.5a O.SS sq"ij2-M-051data

JEnvironmentar lestlng Nomunons, inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Daily Chemical Analyses Client: TVA Test dates: Febni Project number. 1684 / Sequoyah Nuclear Plant, Non-treated rary08-IS, 2005 Reviewed b Concentration lParameter Day 2 I Day3 I Dav4 I Day5 I Day6 I I 4 p11 (SU) DO (midL)\\ I Conductvitv (umhuslem) Inltial I Final I InitalI Flnal I Initial I Flnal 1 7.86 7S 7.721 7.461 7.82 7.54_ 7.8 7.81 7.7 7.8 7.5 7A.2 247 S3 24.5i 25-31 24 I Final I Initial Final I Initial Final 0 7.85 7.961 7.79 8.11 7.39 7 7.1 7.7

7.

7.8 73 282 289 4 461 4 86 9 24.7 2409 2.5] 25.2 24.6 Control ^ I Alkalnity (mnJL CaCO,) I Elardness (mv/L CaCO) 4.-, Temperaturn 1'C) 2.1I 248R H (SU) 7.76 7.51 7.72 7.52 7.69 7.41 761 7.55 7.91 7.85 8.17 779 8.22 7.41 10.98% DO (mz/L) 7.7 76 7.9 7.7 7.9 7.5 7.7 7.5 7.8 7.2 8.1 73 8.1 7.4 Conductivity (jImhos/cm) 286 304 278 271 294 275 279 Temperature CC) 25.2 246 25.1 24.6 25.2 24.3 25.1 24.5 24.8 24.6 25.1 243 25.0 24.6 pH (SU) 7.75 7.52 7.72 7.51 7.67 7.47 7.62 7.55 7.92 7.84 8.18 7.72 8.21 7.42 22% DO (mglL) 7.7 7.9 7.6 7.9 7.5 7.8 7.5 7.8 7.2 8.0 28.2 7.4 Conductivity (Iboslem) 295 276 267 298 265 I 2 69J Temperature(C) 25.2 24.6 25.3 24.7 25.2 243 25.11 247 24.8 24.6 25.1 24.3 250 24.8 pH (SU) 7.761 7.51 7.721 7.541 7.661 7.491 7.61 7.58 7.901 7.87 8.18I 7.75 8.231 7.42 43.9% DO (mI/L) 7.71

7.

.9 76 801 7.6 91 766 781 8 7.1 7.9 7.2 8.2 7.4 Conductivity OLmhos/cm) 2 240 2741 248 2413.1 2X 243 Temnerature (MC) 25.41 24.6 25.31 24.71 25.31 24.41 25.3 24.4 4 .AiJ.l pH (SU) 7.751 7.50 7.711 7.551 7.631 7.521 7.57 7.58 .9 4 -1 - t- t DO (mI1L) 7.71 7.4 7.9 7.61 8.1 7.6 7.7 7.6 72% 4 4j. 4 I - -4 4 . Conaucuvtv lunanosinmi 4-tff Temperature CC) 9-4- pH (SU) 100% DO (mg/L) fi Conductivitq (6enhos/cm) i 4-Alkalinity (mzlL CsCO,) 4-Hardness (mg0L CaCOA) Total Residual Chlorine (mn EL)I Temperature CC) 212 231 213 205 255 205 207 25.3 24.7 25.2 24.5 25.1 24.3 25.1 24.6 24.9 24.6 25.2 24.3 24.9 24.6 7.75 7.53 7.69 7.56 7.62 7.53 7.59 7.59 7.90 7.85 8.20 7.91 8.25 7.48 7.7 7.8 7.6 8.2 7.6 7.8 7.6 7.7 7.0 7.8 7.6 8.3 7.5 75 204 179 174 225 177 177 68 67 82 80 78

0.10

<0.10 @.10 25.4 24.8 25.3 24.5 25.3 24.3 25.21 24.5 25.1 24.7 25.1 24.4 25.11 24.5 7.76 7.51 7.72 7.56 7.61 7.49 7.651

7.

7.90 7.87 8.14 7.83 8.271 7.50 7.7 7.4 7 7. 7.6 8.2 7.7 7.9 7.7 7.8 7.1 7.7 7.1 8.3 7.5 181 201 178 173 223 68 66 68 78 80 80 <0.10 <0.10 25.1 24.6 25.2 24.5 25.1 24.5 25.2 24.5 25.0 24.7 25.3 24_.4 25.0 24.6 4.- 4-pH (SU) DO (mP2L) f Conductivitv (runhos/kmi 100% Intake Alkalinity (mnghL CaCO.) i,;,. 4 Hardness (mnfL CaCO.) Total Residual Chlorine (mgL)i l Temperature AC) _- -- - \\,

Environmental Testing Solutions, Inc. Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Page 5 of 6 Date: na-ce-OS Daily Chemistry: I Dav I I l Analyst I Concentration I Parameter CONTROL pDH (S.U.) 4 DO (mg/L) _~ Conductivity (limhos/cm) I Alkalinity (mg CaCO3L) Hardness (mg CaCOJL) \\ U _,4_ Temperature (°C) pH (S.U.) 77Lo 7 51 I 7Z 10.98% DO (mg/L) Conductivity (ipmhoslcm) 7 Temperature(°C) (Q Z' I.I 25.1 V-6 2S.2. 211 5 .pH (S.U.) '7.7 PS 5Z 7.7 1z-.51 %t7 707 22% DO (mgtL) 7Lo 77 1,5 Conductivity (Ormhos/cm) Z7 1Zq5 ___p_ Temperature (0C) 25 *2. 'ZS.3 21.1 Z5s. 2.*3 pH (S.U.) 7.lp 1.5t 7.72 -j 1tsto 43.9% DO (mg/L) 1.1 77.( Conductivity (pimhos/cM) 7___l Temperature (0QC) n*Y s s.

3 pH (S.U.)

-75 7.50 '7.7 t 7.-L1.oS. r5 72% DO (mg/L) 77qL1 Conductivity Temperature(°C) .*3 _4 _ S.2. Z. 25.'i 2'.3 pH (S.U.) '7.-7 5 100% DO (mglL) -7.' Conductivity (pmhos/cm) 175 Alkalinity (mg CaCO3/L) b_ Hardness (mg CaCO 3IL) TR chlorine C (mg/L) .It Temperature (°C) I pH (S.U.) )I Aq r 100% Intake DO (mg/L) -7 Conductivity (jimhoslcm) l I S Alkalinity (mg CaCOL) lo& Hardness (mg CaCOAL) __8 TR chlorine (Mgo.1C Temperature (°C) 2S.l I ..I I initial I

Vnvirnnmental Testing Solutions. Inc. Page 6 of 6 .,..vir~ nalTstigSltos._ I.....c. Pag 6. of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: o02-c-tS I I Duay I 5I 3 I 4 AnalystI O1m I PCs I PVC T I 5 1 -Attt

Concen-Parameter tration I

CONTROL pH (S.U.) -Lz L DO (mg/L) I Conductivity (jimhos/cm) 29 3 Alkalinity (mg CaCO3/L) Hardness (mg CaCO,/L) N -71 ItoS0" I (L4 I &4 _. (o yw,*1.71 343 "41% 1 Ai-4' ?-fez ecq tI Temperature (C) 24.- 24.S 2S.Z7 LN to V U l~~ ~ _b pH (S.U.) -1 1oI eb.17 B.Z2 t_ 10.98% DO (mg/L) '7.AWzIh R-1 -71 V I r I). . 1 1 I Conucivty _zq 23.Ze3 IN Temperature (°C) 274.5S 7A4. 1_. - 2q.3 1pH (S.U.)

1. ( 2 L

17 Ss 1 47 22% -DO (mgtL) I a 5 Conductivity (gmhos/cm) Z ( 2?8 Temperature (°C)

25. I 7

8 2S.L t

-Z.z 0 I -.18 V5 a 1j3.21 7~ pH (S:U.) . t o I -1.19O 43.9% DO (mz/L) Conductivity (pmhos/cm) IS~ I -. 3, 1. '.9 1.4 AmIt ItZ=ufr II -4=d L 11 T^"orewto,-%f . 6 & _I e-ClUjJl ALUR V~ k %.,I 5.Li~t 7.. £.'Ia £.--t. &Cl7b 72% -DO (mg/L) 7.1 7.9 .03 -7& Conductivity Z.-___ (Pmhos/cm) OS stIt,+ Temperature C) Zs. I 2-4.(O 'Z. SL A-I nUP II-,ntet? 17 <4 rlQt14 Q7n I -A G I I 2, -4 t-^ \\-@J X;& ev VJ. -- 100% DO (mR/L) - a Conductivity (pmhos/cm) Alkalinity (mg CaCO3/L) Hardness (mg CaCO3IL) TR Chlorine (mg/L) Temperature (C) 712 ~c.40Iv .qo.7 i AS R 9 0 DH (S.U.) r A__., l 100% Intake DO (mg/L) Conductivity (mhos/cm) Alkalinity (mg CaCO3tL) Hardness (mg CaCO3/L) TR chlorine (mg) Temperature CC) 7.13 1 -1.7 7.9 22.5 V Ro 40-10 ,ZS,.?- I _W-C -1 IS-0 '7.1) I1S3 .zz. I 2zq. 5 _.8S !&?I 7.5b -1.1 G.3 7.5; re d I I Initial I 5.-5 Z4IZS~. O 4.b I Final I Initial Final I Initial Final i

Environmental 'esting olunons, inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses TVA / Sequoyah Nuclear Plant, Non-treated Client: Test dates: February 08 - 15, 2005 Project number-1684 Reviewed by: le /t 9 9 1 Concentration IParameter Day 0 I Day I I Inltal I Final I Initial I Final I 4 I .1 npl (SU) 7.861 .7.571 7.861 7.611 4 DO (nf )~ I Day 4 1 mli I Initial I Final I InitL 771 7.801 8.051 7.61 7.71 7. 307 ~62

2.

24.5 24.61 1 Dar6 1 Conductivity (jimbos/cm) Final Initial Final 8.13 8.11 7.6 8.0 7.8 7. ~289_ 61 86 24.4 Control Alkalinity (ng/L CaCO_) Ulrdness (mW/L CaCO) 4 TrstlAr ¢ -IC1 II )A 1 I) C ' 24 ' 24.7

us a

wuiIIc J~ J '.s_.,S1.w _. a., pI (SU) 7.76 7.59 7.72 7.61 7.69 7.5 7.61 7.74 7.91 8.061 8.17 812 8.22 7.61 10.98% DO (mnwL) 7.7 7.9 8.0 7.9 8.0 7.7 7.6 7.8 7.4 8.1 8.1 7.8 Conductivity (umhos/cm) 286 278 294 27279 Temperature (C) 25.1 25.1 25.1 25.1 25.1 245 25.1 24.6 25.2 24.6 24.7 245 25.0 24.6 pl (SU) 7.75 7.58 7.72 7.62 7.67 7.58 7.62 7.73 7.92 8.07 8.18 811 8.21 7.60 22% DO (mg/L) 7.7

7.

7.9 7.9 7.9 8.0 7.8 7.7 7.8 7.8 8.01 8.2. 78 Conductivity (Jmhos/cm) 271 295 276 267 298 26 269 Temperature ('C) 25.1

25.

25.1 24.7 25.1 24.7 25.1 24.6 25.0 24.5 24.7 244 25.0 24.5 Pl (SU) 7.76 7.59 7.72 7.64 7.66 7.59 7.61 7.74 7.90 8.08 8.18 8.12 8.23 7.61 DO (mg/L) 7.7 7.7 7.9 7.9 8.0 8.1 7.8 7.8 7.3 7.9 8.1 8.2 7.9 43.9% ConductUvity (Umhos/cm) 240 273 248 241 288 243 243 _Temperature (C) 25.1 25.0 25.0 24.8 25.0 24.7 25.1 7 4.7 25.1 24.6 24.6 24.4 25.1 24.5 -1. -7 7C 1 7 Al '7.71 7.64A 17.6l 7.Sq 7e 5i7 --7, 7o1t II x ( S.lb

8. 14 8.23 7.61 72%

DO (mw/L) I .77.61 7.9 7.9 1 8.1 8.0 7.7 7.7 7.7 7.4

7. E 8.1

8.

7.9 Conductlvity (umhos/cm) ___I_ 212 231 21: 205 255 20 207 IaIa I I remperature (C) Al (SM __ DO (mglL) Conductivity (umhos/cm) 100% Alkallnity (mngL CaCO3) Hardness (napL CaCO.) Total Residual Chlorine (mgL) <O. Temnerature (C) 25.31 24.91 4 nly (MI) 7.761 7.891

A__,

DO (*aLL) 7.69 7 7.621 7.59 7.59 7.73 7.90 8.11 8.20 8.17 8.25 7.64 7.8 8.0 8.2 8.1 7.8 7.7 7.7 7.3 7.8 8.2 8.3 8.0 204 179 174 225 177 177 67 66 80 78 <0.10 <0.10 25.0 24.91 24.7 25.2 24.6 25.3 24.6 24.5 24.3 25.1 24.6 7.72 7.61 7.61 7.58 7.65 7.67 7.90 8.07 8.14 8.15 8.27 7.61 7.8 7.8 8.2 8.1 7.9 7.8 7.8 7.5 7.7 8.2 8.3 8.0 201 178 173 223 183 181 668 80 80 0 2.10 501.10 25.3 24.7

2.

245 52 24.6 250

2.

=

4.

45

2.

24.4 Conductivity (umhiosctm) 4-100% Intake Alcalinity (mg/L CaCO3) Hardness (mg/L CaCOj) Total Residual Chlorine (nlwJL) Temperature TC)

]Environmental Testing Solutions, Inc. Species: Ceriodaphnia dubia Client: Seauoyah Nuclear Plant - Non-treated DaiY Chemisty: Analys Concentration Parameter CONTROL pH (S.U.) S L Conductivity ~~(jmhos/cm)- 3 ~Alkallnity_. f Hardness a (mg~j CaCO3/L) 8l Temperature (C ~ .0% pH (S.U.)

7.

1.98% DO(m/L) '7.1 Conductivity= Page 6 of 7 Date: OZ-ot-OS Ta',o I, I I I 2 a^4 Ca I84_ I tjM 1 P,44 7.-7 7 -7 toq 1 I. 55l-- I -7..7 &Miwl (pmhos/cm)- I. ?.9 e. - ~ P;72 174..Z 1t X. C.n Temperature (°C) I I1? I pH (S.U.) t t

1-

- 22% DO (mglL) Conductivity _,i (limbo lm)- IZ-- Temperature (QC) 2S. pH (S.U.) 711xo _7__ 43.9% DO (mg/L) -Z.2-2 Conductivity (_ ih a o) s/ m) Tempershure (°C) 25. Z-I -7. '11 I , '?.cf. -- - f I '&.U - ?R5 .V Z:i G. -15, i - - 17 toto0 -L19 . % C) 11-75 1. -Zly-o . 75 '7.-7 1 pH (S.U.) -iL t S ^ 72% DO (mg/L) -7.-I 1-97 I I i' q. - I,&I J A- '9.0 I ConductivityI Temperature (°C) I.4 I ILZi Ii pH (S.U.) -7.17S

~

100% DO (mg/L) 7.7 Conductivity (limhos/cm) 17I5 Alkalinity (mg CaCO31L) (_ Hardnes s a (mg CaCOIL) TR chlorine (nig/L) ( ~ ( Temperature (°C) pH (S.U.) O. P 100% Intake DO (mWL) 7.1 Conductivity (Lmhos/cm) 1g. Alkalinity (mg CaCOJL) Hardness (mg CaCOL) 1 TR chlorine (m/L)o. Temperature (°C) 2 IV.

I2S.3.-1 Final I

Initial Final Initial _ I Initial I -lnal, I

Environmental Testing Solutions. Inc. Page 7 of 7 Species: Ceriodaphnia dubia Client: Sepuovah Nuclear Plant - Non-treated Date: 0'2-09-OtS q I Day I II 3 4 I 5 0 - - i a - -~ i_ Analyst I Concen-tration Parameter h CONTR~OL pH (S.U.) DO (mg/L) = Conductivity (6mhos/cm) Alkilinity (mg CaCOAL) O _4_ - Hardness (mg CaCOIL) Temperature (°C) pH (. .) LO .7 _ 10.98% DO (mgSL) 1

7.7 l

.7 21 1_ 1 Conductivity 7 (jumhos/cm) Z -71 l zqt Temperature(°C) I 2S. - 8l l S pH (S.U.) 22% DO (mgL) Conductivity (umhos/cm) \\ r----_ = TemDersture (C) I Iu-pH (S.U.) 43.9% a_ DO (mg/L) 5--O Conductivity (jumhos/cm) Temperature (°C) S pH (S.U.) 72% DO (mgfL) Conductivity (umhos/cm) Temperature tC) PH (S.U.) 100% DO (mg/L.) Conductivity (fumbos/cm) Alkalinity (mg CaCO3JL) Hardness (mg CaCO31L) TR Chlorine (mg/L) Temperature (°C) <0.10 7La.(1 z4.S7 .3 -ZT pH (S.U.) 0.&!5 i P. I67 r-. S;5 .I g.3 Z4Ie 100% Intake DO (mg/L) 71. 1Ls r 7. Conductivity (iimhos/cm) 1173 2.23 Alkalioi3tlo (mg CaCO&3/L) Hardness SO (mg CaCOyfL) I TR chlorine (mgfL) i 4

a. I Temnerature ffC)

-2S. I V2Y. I -),Z A w - Initial I Final I Initial . .

Chronic Whole Effluent Toxicity Test (EPA-821-R-0Z-013, Method 1000.0) Species: Pirnephalespromelas Daily Chemical Analyses TVA I Sequoyah Nudear Plant, UV-Treated Client: Test dates: February 08 - 15, 2005 Project number: 1684 Reviewedby-0 I I-Concentration Parameter p11 (SU) X-I DO (mg/L) Control Conductivity aunhosrem DOaint (AgL) CC3 hardness (mn/L CaChe3) Temperature (C) 25.1 24.61 25.2 24.51 25.2 24.41 25.2 24.6 24.81 24.5 24.9 24.41 24.91 24.4 p11 (SU) 7.78 7.581 7.72 7.541 7.70 7.43 7.68 7.52 7.73 7.79 8.12 7.75 8.20 7Ai 10.98% DO (mg/L) 7.8 7.6 7.8

7.

8.0 7.4 8.1 7.8 8.0 7.1 7.8 7.3 8.4 7.4 Conductivity (pmhos/cm) 277 301 284 1l 279 1 326 1l 272 279 Temperature (*C) 25.2 24.7 25.4 24.6 25.3 24.3 25.3 24.7 24.8 24.4 24.9 24.3 25.1 24.4 II (SU) 7.76 7.5 7.73 7.6 7.69 7.41 7.71 7.56 7.74 7.82 8.13 7.74 8.20 7.39 22 DO (mWL) 7.8 7.6 7.8 7.6 8.1 7.4 8.1 7.6 8.0 7.1 7.8 7.2 8.5 7.3 Conductivity (pmnhosftm) 270 290 271 265 . 306 270 271 Temperature (C) 25.2 24.6 25.3 24.4 25.3 24.4 25.3 24.7 24.9 24.6 25.1 24.3 25.1 24.4 pit (SU) 7.78 7.53 7.73 7.54 7.68 7.40 7.72 7.56 7.74 7.80 8.14 7.74 8.21 7.38 DO (mgtL) 7.8 7.6 7.8 7.7 8.1 7.5 8.2 7.7 7.9 7.1 7.8 7.3 8.5 7.4 Conductivity (pmhos/em) 240 265 237 1 1 244 289 243 244 Temperature (C) 25.4 24.8 25.3 24.5 25.1 24.6 25.1 24.7 24.9 24.6 25.1 245 25.0 24.1 pH (SU) 7.78 7.52 7.71 7.59 7.67 7.53 7.72 7.61 7.75 7.84 8.14 7.84 8.21 7.47 72% DO (mg/L) 7.8 7.5 7.8 7.7 8.1 7.5 8.2 7.8 7.9 7.2 7.8 7.6 8.5 7.2 Conductivity (pmhostcrn) 205 l ~ 217 ? 212 3 1 212 255' 214 214 Temperature (C) 25.4 24.8 25.1 24.5 25.1 24.4 25.1 246 24.7 24.5 25.0 24.3 25.2 24.4 plH (SU) 7.79 7.52 7.74 7.58 7.66 7.45 7.67 7.57 7.72 7.84 8.15 7.84 8.21 7.46 DO (n/L) 7.9 7.4 7.8 7.7 8.1 7.5 8.0 7.8 7.9 7.2 7.7 7.6 ..8.3 7.3 Conductivity (imhostcm) 183 199 183 170 211 185 182 100% Alkalinity (mg/L CaCO3) llardness (mg/L CaCO3) Total Residual Chlorine (rafL) Temperature CC) 25.51 24.81 25.31 24.31 25.21 24.41 25.31 24.71 24.91 24.61 25.11 24.31 25.24. nil (SU) 7.79 7.54 7.74 7.571 7.66 7.51 7.73 7.51 7.77 7.88 8.14 7.77 8.21 7.39 DO (mgfL) 7.9 7.4 7.9 7.71 8.1 7.5 8.2 8.11 8.2 7.3 7.9 7.6 8.3 7.4 Conductivity (pmhoslcm) 1841 200 175 215 174 177 100% Intake Alkalinity (mg/L CaCO3) llardness (mg/L CaCO3) Total Residual Chlorine (mg/L) Temperature CC) 25.1 24.61 25.3

24.41
25.21 24.5 25.1 24.8 25.0 24.61 25.01 24.31 25.1l 24.3

eks owU

Ensironmental Testing Solutions, Inc. Page 5 of 6 Species: PimephalesPrOmelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: 02O0-8-U n - M. J"L - , "-P. Day__ Analyst -L_ L L f4 Concentration Parameter CONTROL pH (S.U.)

1.7oL

7. r

1. ?3 17 i.z

-7.40 Conductivity S q. (pmnhos/cm) q-___ Temperature(°)

z.

Z_.L .zA.S Z5.Z Z'1.' pH (S.U.) 7.n _.7Z 10.98% DO(mn/L) -78 -V* 1. Conductivity (gChosucimi) Z 7 w c9 Temperature (°C) .5.1. 7L .1 2S'4 AM.o 2s.5,3 pH (S.U.) .7LO 7,S1 1.2735 -7... l '7-4 22% DO(mg/L) 178 -.I 7.8 Conductivity (jimnhos/cm) Z70 '7i Temperature (0C) X 4.(

ZS5 2.

pH (S.U.) 7 7 773 .5 1 7.LA 4O 43.9% DO (mg/L) 1$ 17.7 1,- S. Conductivity (pirhos/cm) Z357 Temperature(°C) 2S. 2Te 8 25*3 -ZS. pH (S.U.) 7.5Z 171 -7je. 1 7.53 72% DO (mg/L)

7.

7 1.5 Conductivity Z0_7 Z13 (pirhos/cm) ZS___ Temperature (C) 2S.q Z1&28 2 S 2S. l 2 pH (S.U.) 1.1q9 1.277. 7.5 7 100% DO (mgtL) -79 7.4 7.8 I .1 Conductivity (Vimhos/cmn) 1 Temperature (°C) ZS.S Zs.g 2.3 ZS.2 . L pH (S.U.) 57.q 7 __7_7 1 5_7 7._ 1.tt 100% Intake DO (mglL) -71B I 77 Conductivity (Tmhos/cm) 2S, I Y.__Z _____Temiperature rC) '25.I

z.

S3 2.. tSZ 2. IIlla1a Final Initial Final Initial Final

gijronmental Testing Solutions, Inc. . '-t.-.. i.- I

.z -.

Page 6 of 6

. ;: te fi.- :

e Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: 02-r&- GS

.3 4 5 6 Analyst CA p*V CA) Concen-Parameter trationl ONTROL pH(S.U.) 2L.7 o -ran 7 1z DO (mg/L) O__ 3 Conductivity (Olrhsl/cm) 728 3 _9_Zl Temperature (°C) 25.Z . 2M la

2.

7.Sa A. pH (S.U.) -LQ?8 7-57 7.l13 10.98% DO (mg/L) Ell

7.

Rib 73 Conductivity (pnihos/cm) 2 73 Z. 7Z Temperature (QC) 75-3 Z4.1 z24.8 a -l A 2.3 S a __pH (S.U.) _1._ 1 7 8.1L3 .ZO S 22% DO (mgfL)

8.

KO .1 7.8 -.z B.S 7.3 Conductivity Temperature (QC) ZS.3

q.

2. V00

25.

0 .3 -S. I pH (S.U.) -L-7 Z a 171 __80___._ 0.ZI 1_.3 43.9% DO (mg/) '~ 7.-7 _1. 1 7.l 7.1 -4 S. 14 Conductivity Z4 (gmhos/cm) 2.13 cn4) Temperature (C) 3S.. qI. .b -L l k pH(S.U.) 7.-m ,7%F 7.715 j.z 7.41 72% DO (mgrL)

7.

-7. Z .e -4lo 6* '7.Z Conductivity 2ZI (CPmhosfcimi) 21 g zS 15q 2t Temperature(°C) 2.(.o .W '.S ,S.i 214.3 Z 'UW pH (S.U.) -7. 17 l .657 _.8 15 _ __ I 1._0 100% DO (mglL) .( 7 1.9

7.

-7 1.7 _lo 0.3 l7. 5 Conductivity (pmnhostcm) 170 2ll 1 Temperature (°C) i.3

l. 2LL.S..

z.

pH (S.U.) 7-3 Ž5rn 77 I2 1? -I .9+ -)lzl= r1 100% DO(mg/L) 8.. 7.i 94 Conductivity -Z iS' 17 i + Temperature(°C) z I lq.g I. 24.C, 2K.3 Z5.1 -i-a- -Itia -inal Initial Final Initial Final Initial Final Initial Final a a - £ a U a

Page t5 Environmental Testing Solutions, Inc. Page l of l Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3JL Titrate samples to pH = 4.50 S.U. Analyst Date analyzed D-0I,0 Titrant normalift and n,,,hWninr dot'rntination! = pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml NazCO2 x 0.05)/E (N x 50000)/ 100 ml sample water reference standard ml ml ml = 0.25E NxSx00 .S S.U. number nurnber (E) (acceptable range - 0.018 - 0.022) .3.97-we glI JwL15-) o.Q r2,A IZ 4 _ O. OZ07 10lO Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = htV/TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOQ) (acceptable range (mg CaCOJIL) (ml) ml ml 90 to 110%) 1 Z5 Z.5 100 100 iz-4 zzo c.Lj 10.1 C1 7 Duplicate sam le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) l(S -D) II(S+D)121s 100 number (ml) ml ml ml (acceptable range -

10%)

ibLZi-O-S Ssu jZ Azo ° 71Z-O- ,51 S3.1 .,;,..+ Duplicate D 2.-

3. l Mfatrix spike recove Reference Spike value Sample l Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CuCOA/)

(mg CaCOJL) (ml) ml nl l I ml l IM.s. zS I 1tE Izso 13.1 .q IJO.iI S Sample alkalinity (B) Measured spike value (IV) % R MV / SV x 100 (mg CaCO3/L) MIV = A - B. (acceptable range (mg CaCOL; 75 to 125%) Sample measurements: I Sample volume Begin End Total Alkalinity Sample number Sample ID l (ml) ml ml ml Multiplier (mg CaCOs)L) lOnC Slto 100 1 qq n Ij jCWQ r.. Q 1 A I I itiI I i I I -'I da I. 7I - N- -I--- I - 1 L i I I I -1 I l2.-D3-O65 4 bz-o4-- o5 _ztu O-ddI Id ILLa-Z97 I + -4 ' ' I ___________________ I + I 14.n 5.7I -'-1 I I __I +_ A 172-I -J I I I. L-- I 1CN I-I 1 C I II .L I I-N-

4 l4

,I I I .I I Rcviewed by: Date reviewed: I 0 -.b 2

Page b'~ Page l of LI Environmental Testing Solutions, Inc. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst OAi-) 7 Date analyzed [4K2 {j-j5Ij Titrate samples to pH = 4;50 S.U. Titrant normality and multiplier determination: pH of N'ormality Normality (A) of H2SO4 pH Factor or 1vulliplier Deionized Titrant check Begin End Total (5 ml Na1CO3 x 0.05)1E (Nx 50000)/ 100 ml sample vater reference standard ml ml ml = 0.251E N s500 = 4.5 S.U. number number (E) (acceptable range- 0.018 - 0.022) 3C'V1 Al I I iZI RIZ 15' 0,0_ _4 12_4 i Laborator control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOAL) (acceptable ranne (mg CaCO,/L) (ml) ml ml ml -90 to 110%) imSS Z5s 100 100 q-1 V. Duplicate sam le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) I(S - D) /I(S+Dy211 s 100 number (ml) ml ml ml (acceptable range -

10%)

bZ-06-os 5Ae 1t .Z2.o~ AZ iI-. i . l -t >X Duplicate D D Z l Matrix spike recove ry:

Reference Spike value Sample l Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOA/L)

(me CaCO3IL) (ml) ml ml ml Sample alkalinity (B) Measured spike value (;1V) '% R MV / SV x 100 (mg CaCO31L) MV - A - B (acceptable range l (mg CaCOIL) 75 to 125%) 14~) too-(. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) nml ml l ml Multiplier (mg CaCO3/L) 0D2 O -os IZ4S 3 4ii l Lo. Is

o. I' ty

-l 0.02-to-S O j i1 s-r. Lo Vi -t__ 6°--° 3SS J. A(I l11 l a 1 z L° I Lp I 12 I SSW f-12tb I___ I l A5 35 0z-11z-oS vn 1f4S Ti Io l l_ L m3.5 oz -iSl 9 m4s 9-zO UIZ. U13 SS CQ +-LO ILtL q .3 I s 33 1 05b2O6DO ADsA I I11 244 4j 5I 1 4 Z I 21q.1 1j + I_ 5c Reviewed by: Dae e d I Datereviewed: I 6'Z-1+06 -1

Page w I Page .Z of Fnvirnnmental Testing Solutions. Inc. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst O J Date analyzed 14-bS Titrate samples to pH = 4.50 S.U. mL Q ali ant multiplier determination: pH of Normality (M of HIS04 pH Factororhultiplier Deionized Titrant check dB Total (5 ml Na2CO3 x 0.05)/E (Nx 50000)1 I00 ml sample water reference standard ml ml 0.251E =Nx 00 4.5 S.U. number number (E) (accepta .018 - 0.022) Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS - MV I TV X 100 standard number (TV) volume Begin End

Total Multiplier (nmg CaCOV1L)

(acceptable range (mg CaCO/L) (ml) ml ml ml -90 to 110%) w1555 Z58 100 100 zq:3 qq 0q q/ Duplicate sam le precision-Sample Alkalinity %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3IL) ((S -D) /[(S+D)121) x 100 number (ml) ml ml ml (acceptable range *- %) D)z12.15 tWAS.4S 3 - 3t1 S Duplicate AMatrix spike recovery: Reference l Spike value Sample l Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3L) I (mg CaCOV1L) ml ml _ml l.S S 19 5° 1 00 dt Q 51, lo,(, 10,1 _110:7 Sample alkalinity (B) Measured spike value (MV) % R =MV SV x 100 (M CaCOVL) MV = A - B (acceptable range (mg CaCO3IL) 75 to 125%) 5 .oo. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOJIL) 05b2U02)Z Oot) 1Z0 I e5 3 ) 2 2 la nSZDD -7 i 4 zb.2i7 1Ob0 -11, 4 3 z1 1-214 4scZ0. 0 I Z. I °StIZlt') 2 o z-s tZ 7I.Z.9 3 _2__o._ Z 28.q Z-9 _____29 Reviewed by: I 6 -1 W Date reviewed: F-OZ -m. Os -1

Page VS Page .5 T ofTq Environmental Teqtin n Snlntinnq-Tnc. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3JL Analyst Date analyzed 1 Titrate samples to pH = 4.50 S.U. .iJ~tcnrality and multiplier determination: pH1 of 0 N b'ormality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin I = (5 ml Na2CO3 x 0.05)/E (NIx 50000)J 100 ml sample water reference standard ml ml ml 2 .25/E =Nx 500 4.5 S.U. number number (E) (acceptable range - - 0.022) Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mgC2CO3/L) (acceptable range (mg CaCOL) (ml) ml ml .ml - 90 to 11O%) IASS ?.S%. 100 100 st's 38,5

R(

l 9C7 / Duplicate sam le precision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) {(S - D) /I(S+D)121) x 100 number (ml) ml ml ml (acceptable range - 10%) b 2v) Y.-L es.ŽiY L S 1 2-0 2+/-.. _ lb.______ Duplicate A 4 5.6 4 7 V 3 D 145 7, 4 /V MAatrix spike recovey__________________________ Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier l(mg CaCOIL) (mgCaCO0JL) l(ml) lml i ml 1ASS' 2,6S? So to 06 f6c 7 i qt to.\\ l v Sample alkalinity (B) Measured spike value (MIV)l % R = MV/ SV x 100 (mg CaCOIL) MV - A - B (acceptable range (mg CaCOV1L) 75 to 125%) l loo. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) lnl lml ml Multiplier (mg CaCO3JL) nb-bz q,2 M ILL: 2 1 0D I.0o 1_4 1_I t)51)2.1 jt)J V 1 i3 Z3. z. 1 _89 C5tz CJbZ¢;SQ 101 t .1 1-)bVA.*Z* I Z _ t1.9 i. 5( 4' I M IIz -C3l 4-3 I 3.l .0F sT I 1~~~~ ____).Z .3-8 o.3 1Q ___z ___1 l~° I5 2 bq -71. ( J>' J2 (e. 6Mb 2.1E

1. 0_

4.

.0 52.7 __ 59o 0.0 Reviewed by: Ia Date reviewed: I 02-Iq-51

Page Page 4-ofJ ' vnvirnnimental Testinty Snlutinns. Tne. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst l Date analyzed D2-l I-In I Titrate samples to pH = 4.50 S.U. u/tiipiier determination: pH of Normal Normality (N) OfH2SO4 pH Factor or Multiplier Delonized Titrant check Begin End -tal (5 ml Na2CO3 x 0.05)1E = (Nx 50000)1 100 ml sample

water reference standard ml ml ml

=Nx500 =4.5 S.U. number number (E) (acceptable range.B022) Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS -MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO~lL) (acceptable range (mg CaCOIL) (ml) ml ml ml -90 to 110%) I45ss 258 100 10' ZZ.- 152-S 01 @tl>i Duplicate sam ple precision: Sample I Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOAL) I(S

D) /I(S+DY2i) x o00 number (ml) ml ml ml (acceptable range -
10%)

L Duplicate D Re.erenc 7.& o5. 0 [ Matrir spike recovj_________________________ spSpike Value Sample Spike alkalinity (A) standard number (SV) volume l Begin End Total Multiplier (mg CaCO31L) I (mg CCO3L) ml ml ml I Z0 ZS --. -0 19-It~ I I I z-oo I ff I,.ll\\9 )',* 1 1G° I l Sample alkalinity (B) Measured spike value (MIV) % R = MV / SV x 100 (mg CaCO/lL) MV = A - B (acceptable range (mE CaCOJL) - 75 to 125%) .~7. l 70

l.

qD ]q Sample measurements: Sample volume Begin End Total Alkalinity Simple number PUwJ-pmaple ID (ml) ml nml ml Multiplier (mg CaCO3lL) 0__ &5Z703 7 3.' IA I(fl) TSO l_ licq Il 0 l1 1 11 I l (6LI.D '1 3 I l Il l Re--eDed b: De rv lie w Reviewed by: I At 7-1 V%. Date reviewed:

Environmental Testing Solutions. Inc. Page I of I....... Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed l 02L -7. Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) ofEDTA pH Factor or Multiplier reference standard ml ml ml = 0.21E

(N xs 50000)/ 50 ml sample number number (E)

(acceptable range - 0.0t8 - 0.022) =Nx 1000 ip?. I ZSII PS$5 ^1Z O.0 q~q

0.

D ozow -- 2O. Laboratory control standard: Reference True value Sample Hardness (MV) % RS - MV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOAtL) (acceptable range (mg CaCOAlL) (ml) ml ml ml _9010 110%) IkL56 Z7-1 40 50 q.4 .I& 2LO 70-2 40 Duplicatesample precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOilL) ((S - D) /((S+D)f2ll x 100 number (ml) ml ml ml (acceptable range - 10%) 92.. 4 Duplicate j D MatrLr spike recoverv Reference Spike value Sample Spike hardness (A) standard number (SV) volume l Begin End Total Multiplier (mg CaCO/L) (mg CaCO)IL) l ( lmll ml ml IO-SS ZZ-40 JU-2) 4z;-.7-L &.2 20.ZO ~ Sample hardness (B) M teasured spike value (MV) % R MV / SV x 100 (mg CaCO1tL) MV= A-B (acceptable range (mg CaCOJ/L) e75 to 125%) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOAL) Blank l______ l (sbou1dbe-OmgCaCO/.L) O. DO I .0 ZO.Z All) l C-l-0 l SthLA IO 22+- Iz4+/- l -7.0 4 l 02bISl LO l l2 4.2 l_ l l D-03 g^ 2X°) Z l Z'll l 2-U-AJ 1 2.0 t 1 l-l 4A S _l_ _ll lzz lll Note: If >15ml oftitrant is used. sample must be diluted. Reviewed by: I1r Ikj Date reviewed L I Ž IZS

Environmental Testing Solutions. Inc. Page__l <,,ot Total Hardness (EPA Method 130.2) Matrix: Water, MDL 1.0 mg CaCO3IL Analyst l Date analyzed [ 07I-ii-Cq -0 Titrant normality and multiplier determination: Titrant Normality check Begin End Total - Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.2/E (i x 50000)1 50 ml sample number number (E) (acceptable range - 0.018 -0.022) =Nx 1000 Ri RS5$ t- )0 V V L 0. Laboratory control standard: Reference True value Sample Hardness (MV) % RS - MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3./L) (2CCCptable range (mg CaCO3/L) (ml) ml ml ml -90 to 110%) y 5 21 40 50 1-q )(.q Z.o ZO.4 '41 IOZ.S Duplicate sam ple precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) ((S - D) If(S+Df211 s 100 number (ml) ml ml ml (acceptable range -E I O%) 4 Duplicate 4.4D Matrix spike recoverv: Reference Spike value Sample I Spike hardness (A) standard number (SV) volume Begin End lTotal Multiplier (mg CaCOA/L) (mg CaCO,3L) ml) ml ml ml Sample hardness (B) Measured spike value YMV) % R M MV /SV x 100 (mg CaCOJ/L) lMV - A - B (acceptable range (mg CaCO~fL) l 7 to t25IM.) { 5o 35 to 259) Sample measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier -(mg CaCO31L) l (should be-OmgCCOA/Lr)l ____ l 0.0 lO 0-0 l /lJ/ 1 2-toos MAS t410 .4-Aj 4 I-r) M Ks 4c 123% 4?2Ao--S.2. 4,ZtZbi {@ IA5 D tl 4b.% 42.91(5 .2-0l 8 I 5020641(O D.Z. A I D._ I I c0zf6 Z ZA a ____I_2_ 2.' '51 cb-'ZJ2. 1¶ + 3 51P Note: If > 15ml of titrant is used. sample must be dilutcd. by: Revieewed I \\ Date reviewed LI~I

Environmental Testing Solutions, Inc. Page of,5 Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst eA] Date analyzed L);i A.+II5 Tirran lier determination: ITitrant Normality check Begn al Normality (N) of EDTA pH Factor or Multiplier reference standard mnl l ml m 0.21E (N x 50000)1 50 ml sample number number (E) (accept3 r =0.018.0.022) 1NX 1000 Laboratory control standard: Reference True value Sample Hardness (MV) % RS = MV ITV x 100 standard number (TV) volume Begin End Total Multipller (mg CaCO,/L) (acceptable range [ (mg CaCO/L) (ml) ml ml ml -900 110%) 40 50 Z4 i / Duplicate sam le precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO)IL) ((S - D) I[(S+D)/211 x 100 number (ml) ml ml ml (acceptable range-* IO%) I)a)Rt RZ5 Z4. 5D 2-2e lo cL30

z; r-t ;

Duplicate D 4-2 [til 3Z2 8 1\\.S2.

4.

a c.- Afatrix spike recovery-Reference Spike value Sample l Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO 3JL) (mg CaCO3t1) (ml) ml ml ml 1 Z$ ZlZ 4t .50 Z I-to B.Ul 13.1 ZOA.4 7 Sampie hardness (B) Measured spike value (NIV) % R= MV I SV x 100 ( .amrg CaCOJL) MV - A - B (acceptable range (mg CaCO.,L) 75 to 125%) LA b _oc Sample measurements: Sample volume BegIn End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO/L) Rlanle I------- l(should be -.O m -CaCO T 0°5t) -Zw~ I 3 D 1 I{D, 1 il-7 J it-Lo ZLVQl 1DE)Di IZ I I 1-U°9 13-19 I1l.C I I H01 05Z3,Z.O3 -3 3-7,q K(P IOJ ZZ 50zO89 _Lg 1 2.3o.1.2 5 i -7 0_ _ __I

2.

5S i4-L _7_ 06Zt Zl -A -3 _____l__3 b°Z-D 2. 01 1k) IX ES I4{5.1. U 1 Note: if > 5m1 oftitrant is used, sample must be diluted. by: Reviewed L A Date reviewed EIj j O s--5

Environmental Testin2 Solutions. Inc. Page Z5 ot

I Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO9L Analyst l 7 Dateanalyzed l 7-ruant noluryaul;itrr ination: Titrant Normality check Begin En Normality ( of EDTA pH Factor or Multiplier reference standard ml ml ml 02/ E (Nx 50000)150 ml sample number number () (acceptable rang 0.022) -Nx 1000 Laboratory control standard: Reference True value Sample Hardness (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJ/L) (acceptable range (mg CaCO3 JL) (ml) ml ml ml 90 to 110%) )SS 7 40 50 145 l(0 zc> ZOA 41 102.51 Duplicate sam le precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) ((S -D) /I(S+DYZI) s 100 number (ml) ml ml ml (acceptable range 10%) 0 0;5 20M21 kAY9 ES V I LE12. Z0 WO.5 2u. 1O.1 iA S az ?.-. Duplicate D Matrix spike recover': Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO/L) (mg CaCO/L) (m) ml ml ml l IASS

Z7-1 it 0 60 79-A*g~l-1 X2C >

.4 2525 Samplehardness (B) Measured spike value (MV) % R= MV /SV x 100 (mg CaCO2IL)

I.

V = A - B (accepbble range I (mg CaCOJL) -75 to 125%) I ~ 1O I Samole naeasurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOi1.) l____________ l(should be -Omg CaCO3/L) \\ X~tb A BIIE 3A l 0 ,0l4 l1tA ? ZA Z t 5C Rn 27 I I001 1 Z Z 7 I - 774_ So I D p3.3-05Z040D3 Sam 1KW T T _ 18.q 1 1 tlsb qg.Z3 I Mg 1g t l I I 1---- -I3 I_ I__._I (.J4I'MJ54JlaLL FICOed I AL~~ L Note: If >ISml oftitrant is used. sample must be diluted. by: Reviewed (I U Date reviewed LZE0. KZJ;

Environmental Testing Solutions. Inc. -4.s /, Pase 58 Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR2S pH/Ion Meter Analyst 1?A1 Date analyzed L)Z Ig.0 I Iodide reagent: 14 13 Acid reagent: l[j, 138 Calibration: - 0.10rmg/L 1.00 mg/L I Reference standard number gI qSS 9-l llaSS t97 1 N~ote: For samples with a residual chlorine or > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (NIV) % RS = MV /TV x 100 number (mgL) (mgL) (acceptable rnge -90 to 110%) Ms I-0.50 &TI'/ Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = ((S - D) I[(S+D)121) x 100 number ( (m~fL) (acceptable range - h 10%) t)5J zbLqe EA klt Cr ELZ e 1AfAl Clit <0.00jSs, 2ss 1 Duplicate D 0 0 1 Samplemeasurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L)

Blank (shouldbe-<0.lOmgQL)

-<i.0 C lg .3L M0 2Y tb . 9flt Pp &L-b &L1b6 O 4 40.oo005zg C2L6E 6 -1v4euSe IAJv AMs C 'fArn __5 O___ 65ZZOO0-.3 O~ oex s*E pp, oo~ OSOD6x.6 Pit ta e' OAC2cs <0.ooreaV 05buo e7 blMU -Y, P,,tnc s o.000 (I b6zoeD oi0 'A,5A PAL6 LUA.J -4C erie es <0.0o0>2.co2 O-4Y3.021 ki' I I 0e"?,.IeNO to XAe 0)$5ow) 0;-, CIt1 o UwC C OL, euW pmepctCls 40, tog I O0 -2.o03 t h ,'geO ffN Cb-,C. 70ARn1icUSS

40. O S.

Note: All samples were analyzed in excess ofEPA recommended holding time (I5 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (MXIV) % RS MV / TV x 100 number (mg1L) (mgL) (acceptablerange-901o110%) Kiss \\t 0.50 {7t(8 j,' Reviewed by Date reviewed

Environmental Testing Solutions, Inc. -5,z /, Page O 2 Page Ij. of J Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mglL Meter: Accumet Model AR2S pH/Ion Meter Analyst OA Lt) I Date analyzed L-Lb o foII lodide reagent: EiiA _ 1I3 Acid reagent: 1 t 13g Calibration: l I 0.1 Omg/L I 1.00 mg/L l Reference standard number I 1£asS I l I)S S lq7 l More: For samples with a residual chlorine of > 1.0 mg/L. the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) MN;leasured value (MV) % RS MV I TV x 100 number (mg/L) (mgIL) _ (2cceptablermnge-90tollO%) WIS S Iq7 0.50 0),t77 qtr *. Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)2 1) x 100 number (mg/L) (acceptable range - 10%) tC2L20z. Sc~- iol PaLL qELwOJ

4.

Duplicate D 0C o Sample neasurements: Sample Sample ID Sample characteristics Residual chlorine number (ma/L) Blank (shouldbc=<0.lOmgfL) J'; U 0'.: C0> Lo x-6GM-ino PALL LL"-j. C.LA Lo.oosF-M2CPF.ZO OUkLV4-1X qrEU-Ow. PARTp CLet-40o.00O 14 05b2JY-.ZI I LUJŽP PALL YelL-P-A TCL29s tiDZlt5otJ '4, - cd Kt CniR.. L IM tlDOSDI L ) <O.o37L2 cDZTJ.DtD 5 . ei f r J COLObL ,t C-Ar., O. Chooo0 O5 Note: All samples were analyzed in excess of EPA aecomncndcd holding time (15 minutcs) unless otherwise noted. (+/-5YZI.oY leizc br-PA1,rICLeES 4oo?42 Laboratory control standard:. Reference standard True value (TV) Measured value (MV) % RS - MV/ TV x 100 number Ir(mg/L) (mg/L) (neceptable range 90 to 110%) AJS5 147 0.50 ,03 '/ Reviewed by l d.ZZ Date reviewed l

Environmental Testine Solutions. Inc. -11 .11 7" Page 616 . Page I of 3 Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.I0 mgfL Meter: Accumet Model AR25 pH/Ion Meter Analyst _ 7777I7 Date analyzed Q?3-IZ] Iodide reagent: l q_ 59 Acid reagent: l lJ& 3a 1b Calibration: I 0.10 mg/L 1.007m-tIL Rererence standard number I (W 5 1jq7 jI 5 l 1 Note: For samples with a residual chlorine of > 1.0 mglL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MIV) % RS - MIV I TV x 100 number (mgL) (mglL) (acceptable range -90 to 110%) l1iffi5 l q 1 0.50 O 5 Z 0: J 07,c)t. Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD {(S - D) l[(S+D)121) x 100 number (mgL) (acceptable range - k 10%) 6621241o bQO~UbAO no 1OrJO WS -Co. S DOo'36,1. J Duplicate D 4 00 3 1 7 Sample measurentents: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) Blank (shouldbe=<0.10rmglL) O. 000 %.0 b lziLCo t EiM~ye.LcLt nc t6Jo io; Y ell co30Z z2.12.OL Ehf~' Uh i \\$ NcO lor, fe1 A ?;LlcA s cooo Z69 (1VA1y.d1 S c L SS f~ 2ob/Sl;fgo+Cesv(oc-5 b5zzl 2.23 O-ClCV Vtr-V % jA;J!, I, 4 l onp. )o. OoZ q2 t5 iZ.zt 7 vbsebaub U-,OT No Colotar o +t\\ e,5 -Co. oo ( 66 06WOZ12,ol d l Amif rnwe %A 6lLk bxoVk OA;c(O' 4o.tfi016 49U, c1Oi wWk F£.felto;} £X..jty j)-ab~5 o,OZ88 006JI 11OZ

1)

+n. ,Uk Wv-ire Yeleoo je,1cacs 0,DZ 88 tnSD 2.Z

1). P wgwj' c

Y&\\Lo 4 bCo01%l, tAa 9w4,clo OO0oI0 Non:: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless othervise noted. Reviewed by z1& \\ z I I Date reviewed l A-( li, 1 I

Environmental Testing Solution's, Inc. Page Page ZL of 3 Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mglL Meter: Accumet Model AR2S pH/Ion Meter Analyst pcB1 II71 Date analyzed OZ - I -Z

o I

Iodide reagent: l I 9 1 Acid reagent: l t 1 IN I Calibration: I 0.10 mg/L I 1.00 mg/L Reference standard number _ NS5 Iq'7 I It jaj7 N~ote: For samples with a residual chlorine of> 1.0 mg/L, the calibration range must be adjusted to bracket the chlorinc levels of thc samples. Laboratory control standard: Reference standard True value (TV) Measured value (MV) % RS =IV I TV x 100 number (mg'L). (mgaL) (acceptable range-90 to 110%) jI$S jq-7 0.50 o04 5 7 9 *.j j Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD - {(S - D) /I(S+D)12l) x 100 number (mg/L) (acceptable range - 10%) 0ob212.P fAco&.ox:ciI S 4 O.o2S6 Do Z Duplicate D -o. 0i 7il Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) Blank (shouldbe=<0.10mg/L) s MO zzol .'4o.,ool71 On2iz.o& Wns 1~-> CS. ?\\Io Lolo(. p~'tcA. <o oco67I 0 2Iz.iO St a .Jo

colot, ew PA%

Je <oo0ooSZ6I1 I bD21z.M I coci J Vlo "poc, $e.0 ;o r <k _o_000_52_ O5D2z..Ll0 B4~SF A1.oz Oro.. c. h~l ^ Zo.~OozZcf Q bz-2.1 t l. ll. U4<GO e, kA--11 6 e o C -ro. ooo V? 7 6- 021 Z 1 ( t ~ i 0 7olo Colt .o f c t ettS <0.000lll 6bb I /3 I.J.u ouh ,°, NC t f e' pos.t coe. 4 _Z_ Note: All samples were analyzed'iA excess of EPA recommendcd holding time (15 minutes) unless otherwise noted. Reviewed by Date reviewed

Environmental Testine Solutions, Inc. I Page_ ____ Page 3 of 3 Total Residual Chlorine (EPA Method 330.5) Matrix: WaterMDL=0.10mg/L Meter: Accumet Model AR25 pHlIon Meter Iodide reagent: l 1tJ q I Acid reagent: I W i: I'S S Analyst fC-5l Date analyzed CZ

I Z -

io-Calibration: I 0.10 mg/L I 1.00 mg/L IReference stnndardnumber 1f l45 )145S IVV Note: For samples with a residual chlorine of > 1.0 rng/L. the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value (TV) Measured value (MlV) % RS - NIV / TV x 100 number

(mglL)

(mgQL) (acceptable range - 90 to 110O/%) [ SS zq~1 0.50

0.

0 .5 o3 I 9 i i Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD - ((S - D) /[(S+D)/21) x 100. number (mg/L) (acceptable range - 10%) LUASA toD C, -, F cleo S eo0oooo3I :b.0::.: Duplicate D cD.ooo73 Sawple measurernents: Sample Sample ID Sample characteristics Residual chlorine number I (mg/L) Blank (shouldbe=<0.10mr/L) I

5 U.

N - -1 Wz11t.D3 No1A$N)ItId Cclor. p~4'~i

46. 6ooZq7

-IrL n D~bZ1zll0q TA ScN-4y 0 Leo rL~5 <.oCeooL60 LbsbZ2lz2 It/ 'F)A-3SF ZW01 A0 40cI& et<.0o135 D¶bzzL' go~i p ) Lcio( -<D100 oZ7 Db-b2i2.D, -P <-000 f'c Note: All samples were analyzed in excess or EPA recommended holding time (15 minutes) unless otherwise noted. Reviewed by Date reviewed

Sequoyah Nuclear Plant Biomonitoring February 8 - 15, 2005 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water Ir1 U -4 Cu in 1.0 0.9 0.8 0.7 0.6 0.5 0.4 1.0 0.9 0.8 State and USEPA Mandated Control Limits Limits Set According to +/- 2 Standard Deviations I I I I I I I I I I I I I I I I I I USEPA Warning and Control Limits Limits Set According to 1 O and 25h Percentile CVs I I I I I I I I I I I I S __ I I I I 0.7 0.6 0.5 0.4 ts. %S-ob t46@ %I,, 'b'VI 'bo ~%-Ts da rp° Nb- -.tr Nt N,.-, NteV OtN b% Test date - day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC2) Warning Limits (mean IC2, +/- S, 5 10)

-----.Control Limits (mean IC25 h Sm or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Phmephalespromelas using Moderately Hard Synthetic Water State and USEPA Labortory Laboritory USEPA USEPA Tt numnber Test date 7-day IC15 CT S Ccntrol Llmits SA10 Waning Limits S.25 Centrol Lmits S5 warnin Limits SAM ControL imits CV (tLXCI) (gILKCI) CT-2S CT+2S CT.SA. CT+S S.1 CT-S CT+SA,5 CT-SA.s CT+SA.5 CT-SMS CT+SSAe 1 0518-04 0.56 2 05-18-04 0.5S 0.57 0.01 0.54 0.60 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.36 0.79 0.26 0.32 0.83 0.02 3 06-08-04 0.61 0.59 0.02 0.54 0.63 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.04 4 06-22-04 0.57 0.58 0.02 0.54 0.62 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.04 5 07-07-04 0.54 0.57 0.03 0.52 0.63 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.36 0.79 0.26 0.32 0.83 0.05 6 07-13-04 0.55 0.57 0.03 0.52 0.62 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35 0.78 0.26 0.31 0.82 0.05 7 07.20-04 0.52 0.56 0.03 0.50 0.62 0.07 0.49 0.63 0.12 O.4 0.68 0.21 0.35 0.78 0.25 0.31 0.82 0.05 8 08-10-04 0.56 0.56 0.03 0.51 0.62 0.07 0.49 0.63 0.12 0.44 0.68 0.21 0.35 0.78 0.25 0.31 0.82 0.05 9 08-11-04 0.64 0.57 0.04 0.50 0.64 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35 0.79 0.26 0.31 0.83 0.06 10 08.17-04 0.62 0.Q8 0.04 0.50 0.65 0.07 0.51 0.64 0.12 0.45 0.70 0.22 0.36 0.79 0.26 0.32 0.83 0.06 11 09-14-04 0.57 0.57 0.04 0.50 0.65 0.07 0.51 0.64 0.12 0.45 0.70 0.22 0.36 0.79 0.26 0.32 0.83 0.06 12 09-21-04 0.64 0.58 0.04 0.50 0.66 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.07 13 10-12-04 0.61 0.58 0.04 0.51 0.66 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.06 14 10-19-04 0.52 0.58 0.04 0.50 0.66 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.07 15 11-02-04 0.68 0.58 0.05 0.49 0.68 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.08 16 11-09-04 0.54 0.58 0.05 0.49 0.68 0.07 0.51 0.65 0.12 0.46 0.70 0.22 0.36 0.80 0.26 0.32 0.84 0.08 17 11-30-04 0.54 0.58 0.05 0.49 0.67 0.07 0.51 0.65 0.12 0.46 070 0.22 0.36 0.80 0.26 0.32 0.84 0.08 IS 12-07.04 0.64 0.58 0.05 0.49 0.68 0.07 0.51 0.65 0.12 0.46 0.71 0.22 0.36 0.80 0.26 0.32 0.84 0.08 19 01-11-05 0.63 0.59 0.05 0.49 0.68 0.07 0.51 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.08 20 02-08-05 0.62 0.59 0.05 0.49 0.68 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 .OS Note:. 7-d1C ay25% hbtion concentation. An estinton ofthe concentratin ofpotawsim chlotidc thatwou cause a 25% reduirn n Pimnepimpls growth forthe test popubtn. CT-Centraltendetcy (mean IC). S - Standard deviation ofthe Ci, values. Labontsry Control and Wanrif Limits Iaboeatoqcontrol nd waninimits wce etblhedusingthe stndard deviation ofthICz vales co pondirgto the 10th and 25th percentie CVs. lhce ranges au more strigent dta thcontroland wamngrlnks recommended byUSEPA for the test method and endpoint SA,- Standard deviation cosponding to the l0'percatie CV. (S.D,. -0.12) S,2, - Standard deviation corresponding to the 25' perenti CV. (SAI - 0.21) USEPA Control and Wardn Limits s - Standard deviation corresponding to the 75' percentile CV. ( SA71-0.38) SAL-Standard deviation corresponding to the 90' percentile CV. (Skt# - 0.45) CV - Coeircient ofvariatbon of the ci values. WEPA. 2tDO tbdatandbgs trAe kisf or MethodVbliy hVhol EbentidbxclyAppFhaties bderthe'tiDbnu ?olhtatDeheartge ltbnPm p lPA-t33-R.0ll). US lsvkomenml Prtn Atgncy. c,,nat OH OtaRlfma ob*l.*Jfro.. Aqv1 t oeSyjfe. I0c 02-05

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0.9 I U 0.8 0.7 0.6 0.5 I I I I 1 1 I I I I I I I I I I I I

A-..- 0.4 F 0.3 0.2 Test date 7-day IC2.= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC,5) Laboratory Warning Limits (mean IC25

SA.10 SAJO = 0.12)

.Laboratory Control Limits (mean IC25. SA.25 SA.5= 0-.2 1) USEPA Warning Limits (mean IC25

SA75,SA.75 0.38)

USEPA Control Limits (mean IC25

SA SA.9= 0.45)

Environmental Testing Solutions, Jnc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water it number Test date Control Survival Control Mean Growth CT .CV CT MSD PMSD CT (%M) for Confrol Growth (ms, (mgflsvae) (°) for Control Growth CV (%) (%) for PMSD (%K) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 05-18-04 05-18-04 06-08-04 06-22-04 07-07-04 07-13-04 07-20-04 08-10-04 08-11-04 08-17-04 09-14-04 09-21-04 10-12-04. 10-19-04 11-02-04 11-09-04 11-30-04 12-07-04 01-11-05 02-08-05 100

100 100 100 100 100 100 100 100 100 100 97.5 100 100 100 100 100 97.5 100 97.5 0.718 0.681 0.859 0.700 0.610 0.805 0.727 0.490 0.673 0.682 0.825 0.700 0.759 0.757 0.756 0.609 0.689 0.687 0.797 0.687 0.699 0.753 0.739 0.714 0.729 0.728 0.699 0.696 0.694 0.706 0.706 0.710 0.713 0.716 0.709 0.708 0.707 0.712 0.710 4.4 9.3 1.3 15.1 11.1 10.2 15.6 11.6 3.6 7.4 12.9 15.8 12.9 1.1 10.9 7.8 15.7 7.6 8.7 3.9 6.9 5.0 7.5 8.3 8.6 9.6 9.8 9.1 9.0 9.3 9.9 10.1 9.5 9.6 9.4 9.8 9.7 9.6 9.3 0.08 0.12 0.08 0.12 0.08 0.12 0.17 0.07 0.12 0.10 0.13 0.12 0.17 0.09 0.15 0.11 0.10 0.09 0.10 0.11 10.5 17.8 9.6 16.8 13.6 15.4 23.4 14.7 18.4 14.4 15.4 16.4 22.1 12.3 19.3 18.0 15.1 12.7 13.0
15.5 14.1 12.6 13.7 13.6 13.9 15.3 15.2 15.6 15.5 15.4 15.5 16.0 15.8 16.0 16.1 16.1 15.9 15.7 15.7 Note:

CV-Coefficient of variation for control growth On average, the CV for contol growth is 9.3% in Environental Testing Solutions, Inc. Pimephales. chronic toxicity tests. Lower CV bound determined by USEPA (10e percentile) - 3.5%. Upper CV bound determined by USEPA (90' percentile) - 201% MSD - Minimum Significant Differcnce PNISD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimurn percent difference between the control and treatment that can be declared statistically significant in a whole efflhent toxkity test On average, a significant difference occurs for Enviromnental Testing Solutions, InC. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.7% from the control. LowerPMSD bound detennined by USEPA (lO percentile) - 9.4%. UpperPMSD bound determined by USEPA (90' percenile) -35%. CT - CenTtral Tendancy (mean Control Growth, CV, or PMSD) ower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests. ?A 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elinination Program.

833-R-0003. US Environmental Protection Agency, Cincirmati, 0}L Organkrms obtabedfrom Aquatic BoSystems, Inc 02-08-0S

Environmental Testing Solutions, Inc. Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 1.25 E 3 o es a 1.00 0.75 0.50 I I I I I I I I I i I I I I I I I I I I Minimumn Acceptance Criteria (0.25 mg per surviving larvae) I I I I I I I I I I I I I I I I I I I I 0.25 0 CU 40 30 20 10 0

.1 I r T

I I r I I I I I r I I I I I I North Carolina Acceptance Limit (< 40.0%/9) Kentucky Acceptance Limit (< 30.0%) .I*.......................

I I

I I...........................I Test date Control Growth or Coefficlent of Variation (CV) Central Tendency (mean Control Growth or CV) Control Limits (mean Control Growth or CV 4 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimep hales promelas using Moderately Hard Synthetic Water ,I I I I I I I I I I I I I I I I I 40 _ 30 t .20 10 n I I I I I I I I I I I I I I I I I .I I I V O Ow 0 t 0 +%:Tes tda tv ent rp, 09 %1t^ N N s°' ,O so Test date PMSD - percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean PMSD) Lower and Upper PMSD Bounds Lower PMSD Bound (O1" percentile) = 9.4%, Upper PMSD Bound (90' percentile) - 35% (Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)

Environmental Testing Solutions, Inc. Page 1 of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas PpKCICR Test Number: .5 Dilution preparation information: Comments: KCI CHM number: CIAt %b7 Stock preparation: so g KCVL: Dissolve 50 g KCI in l-L Deionized water Dilution prep (mgtL) 300 450 600 750 900 Stock volumc (mL) 6

9 12 15 is Dilucnt volume (mL.)

994 991 988 985 982 Total volume (mL) 1000 1000 1000 1000 Test organism information: Test information: Organism age: 22.1S 2M n Sl A) CAtX. Randomizing template: L1t Date and times organisms 07-c1-os Vbc5 1 tO Incubator number and were born between: shelf location: Organism source: 'S& &W 02-crn-OS Artemia lot number: j&iOOS& Transfer bowl information: pH= SU Temperature 0C Total drying time: .1.6 7 b% 2A-Date / Time in: c>2-0 c t'4iSC Average transfer volume: Q Date / Time outkj.i1.*c : 20 .__ Oven temperature: b Daily feeding and renewal information: Day Date Morning Afternoon Test Initiation, MHS Analyst reeding feeding time renewal, or batcb used time termination time 6724t 015 t 40 i41l02-06£5 c l 2-OecS ot3Z S3

jjl O2-onSc II I O2z s

c637. V 5oO LI 1'A lot.-oL~-6-S i 2 cnlL-oS o0q_ IS30 lIA 0-o--os 3 aL -~ oizvs 1I5O Iq50C tZ-c31-cb 4 at-a2oS iPI 2C I53 nL43 o.Z lb 65 B 6 o-tiqos oi-I'____ 1to0 0Z-10-s Os 6 aL-l(4-Os 00ILs I %1Cm I:5 027 l 2*OS 1<K 7 azl = "-' - : Control information: Acceptance criteria Smmary of tst endpoints: .it 5 I. s 20% I 7-day LCso a

t.

Z Average weight per initial larvae:

16. l 917

. ^.;, NOEC IYSO___0 Average weight per surviving larvae: 0.1 O lo > 0.25 mg/larvae LOEC (am ChV 51.6. IC25 1,/.

Environmental Testing Solutions, Inc. Page 2 of 5 .Species: Pimephalespromelas PpKCICR Test Number: S3S Survival and Growth Data Day Control 00 m KCVL 50 mg KC A B C D E F G H I J K I L 0 lo o0 10 10 10 ID IC 6 10 10 o 2 10 / 0 0 /0 / 010 1 10 lo /Olo 2 to I to 10 I1to 010 lo to 1010/10 3 /O IC)IIC I C IC 10 10 1 O IC) 16 O IO / l C 10 I0 10 1 0to /0 I0 10 10 1 I 0 /0 6 tl o 1lO 10 10 10 to lb O I 10 w) c ( '01 10 l O 0 'o &) ' ) /) 7O 1 0 I0 10 IC

1) 0 1

10 13 iO A - Pan weight m Tray color co=e \\IgA

1.

o Y6 s <IS 5I s }lq7IS.5Z6 Ys 1i51 Analyst: PCLB I ,qiso 'q.6,3 I71 If-p 16.'50 j-1 7' /19,s 1',63 Iq 's5 1j. 7( i S.1 B - Pan + Larvae weight (mg) A \\ A V i O0 Analyst: fa ~ r P Larvae weight (mg) - A - B ^ Da oh e & 4'~ 've^ \\V

4.

2( v WClght per Initial number o l a rlarvae (mg) 10,' O 7 Average Percent l# weight per reduction O O.b33 Initial from control number of (%M Corment codes: c = clear, d = dead, fg fungus, k = killed, m missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Page 3 of 5 Species: Pimephalespromelas PpKCICR Test Number: 53 Survival and Growth Data Day 600 mg KCIIL 750 mI KCIUL 900 mig KClL _i_ N P R S T U V W X a a 0 t 10 I)0 10 0 I /6 O (0 I q 1,A 41__ 3 I '1s 9 4 8' 5~~ o _ __3 5 O I Z 3 r 4

9 9

R 8 1 1 6 6 2-S Z 3 LA t 6_ ? 1 8 D LO_ S 3 1 5d 3 1 0 5 3 I 3 A - Pan weight (mg) Tray color code:: Analyst: I i'f,*57 i'f.qqZ V 1.'5i4 _ t1'473 1 6 Lll s.M j,1 Iqlo B - Pan + Larvae welght Analyst: V Larvaeweight (mg) - A - B Weight per Initial number or larvae (mg) 'V 16 A ~ C / Inital number of larvae '.5 O "2 h s O Average Percent weight per reduction ',.e1z number of (%) Iarvae (mg) Comment codes: c = clear, d = dead, fg fungus, k killed, n = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test somben PpKCICR 9 93 (#53 at 351 Depot St.) Test datess February 8-I S. 2005 Revetwtd by (Q C'-{h.s.L R _Pk. tew rw 6.E1 B t .U-L LN-.t(O w WftMI S0* M-"bU.. 3 h5 CWd-d. .1.d-M.i A4. 1 dghUdw C..mei. d tc 1_e .. ItUO .A.2 3 ewfl-t-s hm _ 1e b(. 0 CIA) 3 Mt rt 3) A 0o 10 14.49 21.33 7.04 0.704 0.704 c.t,3oA-- 3 9 13.01 21.91 6.90 0.767 .0 0690975 061.9Ntapeb Control C o9 l46 s 70 .0 0.706 69 S9_7 5 n 0.6t7 3.9 Not applicble C to I30 14.63 21.68 7.05 0.701 0.705 D 10 to 14.79 21.27 6.41 0.648 0.648 e I30 0o 11.31 22.07 6.72 0672 0672 300 F I0 30 15.50 22.67 7.17 0 717 0.633 12.2 0.717 100.0 0.3 2.2 7. G Io to 14.97 20.40 5.43 0.453 0.543 It 0o 30 34.53 20.53 5.98 0.59 0.598 10 3 0 14.63 21.75 7.12 0.712 0.712 450 .1 0t I0 34.53 20.33 3.78 0.537 0.633 30.4 037 00.0 0.633 10.4 7.8 K 10 10 14.71 20.50 5.79 0.579 0379l t0 t0 31.38 21.33 6.6

0. 663 o0663 I1 0 9

34.57 20.62 605 0.672 0601 600 I 0 7 14.92 20.60 5.68 0813 0.695 12.4 0.568 ns s37 84.7 23.7 o lo 7 34815 19.09 4.24 0605 0.424 o 0 8 4.89 20.42 5.53 0.691 0.553 Q 0 7 144 38.536 4.02 0.374 0.402 750 It 10 6 14.61 37.87 3.26 0e343 42 0.326 600 0.332 1J7.4 3.7 S 30 6 14.73 38.33 3.38 0.563 0.333 T 30 5 14.54 17.15 2.61 0.522 0.261 83 3 0 0.00 0.00 0 00 0.000 0.000 "O V 0 3 1469 16.94 2.25 0.750 t410.2217.s 0.119 92.1 2 W to 3 14.97 13.50 0.3 0.'I0 0013 xI__ X 30 3 34.70 16.69 3.99 0663 0.,99 Dusnttn MS) VOaet PMSDI 0.8U43 35S.3 MSD - Mlnimut Significant Difrerrnee PFASD - Percent Minimurn Sivnfcsn't fiffervoce P~SD MSD IramIe,! ortc tprecisio. The PMSD 18 heminimm perent d iffernce betw een the conbt ant d Oetitnentt thtcm bie declucd suiticuialyiignltlcm 1e8 u~toleeltten osliryten On Ymnrce; s ignificant diftcrence ocetfor nrnint Testing Sdouion, Inc Chronie loxichy tests when a loxicant feducet Pmepholes gvowth by 15.7% hot the control (detennined through weretnce toecant testingN LoerPMSD bound detmined by USErA (lOth pecle) - 9.4%. Upp ?MSDbondderminedbyUSEPA t90th peremile) - 5355 The lowerand upper bounds wee calcuated tby the UJSEA utiig2OS tett conductd oen 19 labortes fo Pimephaet uthin chrtfic itrctnee toiecant tesm USEPA 2000. UtIdetntding nd Acounting fotMehod Viability I WholeEfentToxkityAppletion UndetheNadotmul Pollutant Disclmpe Elimninatlon Pro n EA33-R4-00. US nirmnenu ProtewonAgtcy.Cinnsmai4038 Orpe.sfms obtakerdjmm Aqualtk it[*S lvem. A.c. 02-0S-05

Environmental Testing Solutions, Inc. Statistical Analyses Lsul PFish GQwtk awl SwrvdITwt-7 DaysSrvyal Swrt Ds&W 2112Cf5 Tut ID, ?pKCEI Samplc ID, PM-Rfbcktlan Ind Date 2tl5005 tab M. EM~fth. Tat4ng Sot Sample fpec 10..Po US&IuM c3*kgida SEaple Date Photocol PWCHRPA-921R4020t3 Tat Speciw PP-Pknephales prya C7omoents Cose-towL 1 2 3 4 D-Control 1.0000 0.90DO 1.0000 1.0000 300 1.0000 I.OXO0 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000. 600 0.9000 0.70G0 0.7000 QtOO 750 0.7000 0.6000 0.6000 0.5000 900 0.0000 0.3000 0.1000 0.3000 Tramfrrm: AmIs Squars Rost Risk I-Tsled Nunibar Tabi Conc uog/L Mean N-Mess Mesa MUb Met CV% N Sum oiteal Reap Number D-Confml Q9750 1.OO 1.3713 1.2490 1.4120 5.942 4 1 40 300 I.ODOD 1.056 J.4120 1A120 14120 QO.D0 4 20.0D 10.00 0 40 450 1.0000 1.0256 1A120 1.4120 1.4120 0.000 4 2QOO 10.00 0 40 600 0.7750 0.7949 1.046 0.9912 1.2490 11.294 4 10.50 10.00 9 40

750 0.6000 0.6154 0.1172 0.754 0.9912 9.469 4

10.00 10.00 16 40

900

.1750 0.1795 0.4100 0.1518 05796 50.476 4 10.QO 10O. 33 40 AsdlbryTest, Staletle Cdtles Skew Kort Shapiro.Wi Teatia dats tzwm= Idztutio(p>0.01) 0.907154143 0.tS4 4.219QZ93 1.131502196 Eoaosy odvrnene cannot be coafkmed l ela i Tat (44 .0s) NOEC LOEC COV TU Stels LUDy-One Rink Test 600 750 670.t203932 Treatnesb vs D0-otfrol aISasxiena I Wih d-.Prbit Parmaerw Value St 95%FldweaLId ~b Control OlSq g rItkl ?-value Mu Sigma Itir SbIpe 10.35701t73 1.755412766 6.91640964 13.797627S1 Q025 4.179123377 7.8m472773 0.24 2.499507 Q0965528S1 7 Intercept .24.8023163 5.039534534 -34.6798042.14-92482t4 TSCR 0.009137572 0.010006611.0.01047539 0.02t750531

10 Poist Probit ax/L ff FiduasILleuies CA:

ECOI 2674 449.6596479 345.9492516 515.1547081 ECO5 3355 523.2197752 432.50t2959 579.2695757 0.8: ECIO 3.711 567.336t3t 4M6.529472 6174t20337 0.7 ECIS 3964 599.0021397 526.195S343 6453441972 C2.O 4.151 625.5161895 559A724111 669.03069S1 a C.t EC25 4326 649.1956165 5S9.0tt0242 690.7511672 BC4O 4.747 7129155964 6M5.9t26075 754.14101t2 0.4 EcSO 5.000 754.22229 711.3186171 801.387C118 EC60 5.253 797.9221581 754.3014312 157.735m774 0.3 BC75 5.674 8762407294 *21.6995503 9719t5371 BCso 5.842 909.4115496 847.710873 1023.7S353S .2 ECIS 6.036 949.664373t t7t.7559766 1081947116 0.1 EC90 6212 1002.t52335 911.1162952 117&.101439 0.0 EC95 6.645 1O87.213545 978.4101491 1325.616076 1 1 100 1000 IoooW EC99 7.326 1265.071677 1099.910896 1657.70172? Dos tnglL Dosee-RieopwPbot Ot'animse~ vl'6alned frm Aquatic BoSysf em:, Inc. 0.1O 0208-05

Environmental Testing Solutions, Inc. Statistical Analyses L a Fi CG. d Sirvivx Tnt.7 Duy Grwth StIM Dast 2205 Test ID PpKCR Su&mpk ID: REF-RrfTaxikaa EId Date: 2t1/2005 Lab DM ElSdv. Tnthig Sot Sunpe JType: pobsshz cb-tk Sapk Datae hotowk FWCHR-EPA t21-R-2-013 Test Speci PP-icpbaks pt meks Caae-aL 1 2 3 4 D-Controt 4X7040 0(6900 0.7050 0.6430 300 0.6720 0.7170 0.5430 0.593o 450 0.7120 o57o0 05790 06630 6C0 0.6050 .o560 0.4240 0.5530 750 0.4020 0.3260 0.3310 0.2610 900 0.QO 0.2250 0.0530 0.1990 Traau = UVefratufrwed I.Talled Isoulk Cosc-eu/L Meso N-Meo M A o Mb Ma CV-h N -stat CridCa1 MSD Mean N-Mm DWConfMI aa686t 1D 0.3363 0.6480 07s050 3.t92 4 0.6S68 1.0m 300 0.6325 0.9210 06325 0.5430 0.7170 12213 4 1.163 2.290 01.063 06323 09214 450 0.6330 0.9217 0.6330 0.5n 0.7120 10432 4 1.151 2.290 0.1063 0.6323 0.9214

600 0.5375 0.7127 0.5375 0.4240 0.6050 14.653 4

3.215 2.290 0.1063 0.75 0.7127 750 03318 0.4331 0.3313 0.2610 0.4020 17.414 4 0.3313 0.4331 900 0.1193 0.1736 0.1193 QOCCO 0.250 92056 4 OL1193 0.1736 AasuifaryTusts Staiteslc CrOil Skw KEIrt SMko.W1p s Test kulcates nozI dz~bktan(p 0.01) 0.952463199 CA4 -040106517 4.63906581 Bdett's Testt bdiates rl wvmu (P *0.41O 2 S91007662 11.344U6675 lhpstao Teet(.fl S0.0) N'OEC LOEC (3V Irv MSD ?ISDp MS8 MSE F.Yrob dr D'mntre Tst 450 600 519.6152423 0.106321484 0.154813325 0.01541772 0.004311229 0.046920247 3.12 Treafmots vs tW.Cntrol Lkssrlatr*klads o C20O R tas pies) P.1t mg/L SD 95% C(1Ep) Skew IC05-190.76 115.23 51.61 63325 0.6529 IC10 473.11 10(.33 43.27 556.90 09349 ICIS 527.19 61.03 230.12 654.70 .1.5762 1o. JC20 5S1.26 36.65 45243 655.79 -0.6506 o.s IC25 61636 23.06 490.70 667.73 .1.2383 1C40 691.46 16.95 630.43 739.18 0.5338 0.3 Icso 741.52 16.05 694.93 79 21 28 0.0177 0.7

hdictst IC estzlas kus thca kwest conccftresm

.0.6' 0.0 0 200 408 600 800 1800 Dose mg/L Dos-tspiuae 11. 0.7 0.6 13 0.4 la 0.3: 0.2 0.1 14all, 0.05 level ofsagrs cmnce Orgaylive obainedfrom Aquatc BioSytmjnu 2r-02-08-5

Environmental Testing Solutions, Inc. Page 4of 5 Species: Pimephalespromelas PpKCICR Test Number: 53 Daily Chemistry: Day Anls 1.I O I eJ -I Concentration Parameter S CONTROL pH (S.U.) A 7.A gl 7.58 DO (mg/L) .7 1,L ^7. 5h Conductivity (rahos/cm) Alkalinity -z (mg CaCO3IL) Hardness ? P (mg CaCO3/L) Temperature 25-3 -Ls..b ?S Z 4.1 (0C ). pH (S.U.) '7.8 a.o S 7,o5 -z -77 J.@0 DO (mg1L). -? 7.-I 1.to 19

1. La 300 mg KCI/L Conductivity (pmhos/cm) 48 (3 Temperature (IC) 7 5I4 A2'Zs..

pH (S.U.) 1.8% -7.o 7.S39 1.54 7.1LP -. 93 DO (mg/L)

7.

i

7.

7 450 mg KCIIL Conductivity (glmhos/cm) N14O IZoO li_____ Temperature 25.O 2q.R vq.S 2SJ 24.q IC ) I _. I_ pH (S.U.) *- 10 7.11 12'

7.

1.77 7 DO (mgL) '11 17.9 S 600 mg KCilL Conductivity (grnhos cm) 1430 144 g t_'I O Temperature Y; 74 Z'S3 Z4.S %.3 'z1,S (0c ) -2 pH (S.U.) 1 AqO 7.11 l 7q 1-Fl 7.Co3 DO (mg/L) ?7.7 7.o ?.9 -1 1 750 mg KCI/L Conductivity (gnhos/m) 17o qo Temperature 2 5 Z 2Y.7 $.53 2q.-1 ?S.z. Zq.S (0 C )Z OH (S.U. ) 1 §O -72Wo 7 -71,.7 DO (m g L 7.1 -7. (P 5jS(-L, q. 7.9 t 900 mg KCUL Conductivity (mhos/cm) 7oc 0 Z: Temperature 2 STOCK (0c) 2ZI)

2.

ZS 1 I 8 71 STOCK Conductivity I____ (Eimhos/cm) _7_____ a mfIo 40 Initial Final Initial I Final Initial Final .1

I I.Environmental Testing Solutions, Inc. Se,: I.- Species: Pimephalespromelas Page 5 of 5 PpKCICR Test Number _ 1 I 1 I I I I I F Day 3 I 4 5 I 6 1.Analyst lCAI flC l PCs C l 1 I_( l

iConcentrationi Parameter CO:TOL pH7(S.U.)

-73Z 77.36 .80 1.8 6 it .!so .g(4

7. 1 1.1

.?. 1.7 Z .8 to s .Conductivity (Alhainity) lot 501 Z (mg CaCO 3IL) Hardness (mig CaCO Af) Temperature S. Z.. o 7 2 3 I.- 1 (OC) ______9

pH (S.U.)

-7 8 .725 3.ID -ii 9.1-4 -7.0 DO (mngtL) '7.q

7.

7t-o I 7t S3 300 mg KCi/ Conductivity (gmhos/cm) 7_q 4 _______777 __0____ Temperature 7.7 Z..4i 2A. * .3 (' C .PR (S.U.) 1-5 t .5

1.

~8 w*

0. 1 7

1 .DO (mg/L)

7.

.3 5 7, Z 7--4. .c3 5. 450mg KCIL Conductivity 130 I&0 Temperature Z.' J. 24** 2

2.

S zs.O pH (S.U.) _.87 7.6 ._600 mg KCIIL. DO (ma.) o7 0 7d 7?T.0 \\ A 'O I Conductivity

itmho*cnU..

1__20 1420 1330 A__go Temperature zs.q J. 5S ZS-C 2q.. Y

24.

- S. O zq.qt .pH (S.U.) '.S7. 50 7.7 Sp Z(O .12 -7.5t1. .DO (mg1L) -1 c) 7.6 1-.6 '7. ' 1. -4,C O l -- A 750mg KCUL Conductivity I I 1,5o __19t1_ Gpmhos/cm) 16_______ Temperature 2q4o Z 2I.4 zq.e i 2q.ej z'I3 pH (S.U.) -.25

.53 7.

900 mng KCI/L Conductivity (gunhos/cm) A*l qq UZO 212.0 Temperature E .S 2Y.9 zqq 2q.j 2l.S 2'Ii 4 STOCK Conductivity c,1/Oo I. Initial I Final I Initial Final Initial Final Initial Final Initial Final I - I - ii - i

Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 1.20 1.15 1.10 1.05 I I I I I I I I I I I I I I I .1l State and USEPA Mandated Control Limits Limits Set According to 4 2 Standard Deviations U U Cu4 1.00 0.95 1 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 Laboratory Warning and Control Limits Limits SetAccording to 1 0ah and 25th Percentile CVs b%1Sro49-O; ,b °S°Ntr~n~s~oS°NooS0XS° 0moO ri, OV °SO90Test date Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC2s) Warning Limlts (mean 1C2 4 SA10 ) - -- Control Limits (mean IC25% SA_25 or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubda using Moderately Hard Synthetic Water Test nluiber Test date 7-day IC1, CT (g/LNaCI) (g/LNaCI) St2te an USEPA S ControlLUnits SA.1 Cr-2S CT+2S Laboratory Waning Units CT-SA.2. CT+BSAJ Lsbor2tary SA.,2 Central Linus CT - S2 CT + SX2 USErA SA.7 Warning Lmtits CT - SAM, CT + SAS USEPA SA9 ContrulLinits CT - SAM CT + SM CV I 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 1I 19 20 08-05.03 09-10-03 10-07.03 11-04-03 12-0403 01-06-04 02-03-04 03-03-04 04-06"04 05-04-04 06-08-04 07-07-04 08-10-04 09-14-04 09-14-04 10-05-04 11-02-04 12.07-04 01-11-05 02-08-05 1.04 1.05 1.02 1.0O 1.08 1.06 1.06 1.07 1.07 1.07 1.06 1.06 1.06 1.05 1.07 1.07 1.06 1.08 1.06 1.05 1.04 0.01 1.02 1.06 0.08 0.96 1.04 0.01 1.01 1.06 0.08 0.95 1.05 0.02 1.00 1.10 0.08 0.96 1.05 0.03 1.00 1.11 0.08 0.97 1.05 0.02 1.01 1.10 0.08 0.97 1.06 0.02 1.01 1.10 0.08 0.97 1.06 0.02 1.02 1.10 0.08 0.97 1.06 0.02 1.02 1.10 0.08 0.97 1.06 0.02 1.02 1.10 0.0 0.98 1.06 0.02 1.02 1.10 0.08 0.98 1.06 0.02 1.02 1.09 0.08 0.97 1.06 0.02 1.03 1.09 0.08 0.97 1.06 0.02 1.03 1.09 0.08 0.97 1.06 0.02 1.03 1.09 0.08 0.97 1.06 0.02 1.03 1.09 0.08 0.98 1.06 0.02 1.03 1.09 0.08 0.98 1.06 0.02 1.03 1.09 0.08 0.98 1.06 0.02 1.03 1.09 0.08 0.98 1.06 0.02 1.03 1.09 0.08 0.98 1.13 1.12 1.13 1.14 1.14 1.14 1.14 1.14 1.14 1.14 1.14 1.14 1.14 1.14 1.15 1.14 .1.15 1.15 1.15 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.1I 0.18 0.18 0.87 0.86 0.87 0.87 0.87 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 1.22 1.21 1.22 1.23 1.23 1.23 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 1.24 0.47 0.47 0.47 0.47 0.47 0.47 OAS 0.48 0.48 0.48 0.48 0.48 0.48 OA8 OA8 OAS 0.4S 0.48 0.48 0.57 0.57 0.58 0.58 0.58 0.58 0.58 0m 0.58 0.5S 0.58 0.58 0.58 0.58 0.58 0.58 0.5o 0.58 0.5S 1.51 1.50 1.52 1.53 1.53 1.53 1.53 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 1.54 0.65 0.64 0.65 0.65 0.65 0.65 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.66 0.40 0.39 0.40 0.40 0.40 0.40 OAO 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 1.69 0.01 1.68 0.01 1.69 0.02 1.11 0.02 1.71 0.02 1.71 0.02 1.71 0.02 1.72 0.02 1.72 0.02 1.72 0.02 1.72 0.02 1.72 0.02. 1.72 0.02 1.72 0.02 1.72 0.01 1.72 0.01 1.72 0.02 1.72 0.01 1.72 0.01 Note: 7-d IC29 - i7day 25% inibition concentration. An estimation of the concentration of sodiumn chloride that would cause a 23% reduction in Certodaphnia reproduction fbr the test population. CT - Censtnl tendency (mcan BC2,). S - Standard deviation of the IC2, VasL LaborAtery Contrel mad Wanring Units Laboatorycontrol rad vrning liits wor estblishedusing the standard deviation ofthe IC2, values eoncvr ding to the 10th and25tlpercentile CVs Tbes ranges are more stingentt than the contol nd waming limits recommended by USEPA for the test method and endpoint. SAra Standard deviation corresponding to the 10" percentile CV. (SAra - 0.08) SAjs - Standard deviation corresponding to the 25* percentile CV. (SA,1 - 0.17) USErA Control and Warning LUnits SA.7" Standard deviation corresponding to the 735" percenble CV. (SA7, -OA5) SA.- Standard deviation corresponding to the 90* percenble CV. (SA, - 0.62) CV - Coefficient of variation of the IC2s values. USEPA 2000. Undersbnding and Accounting forMethod Variability inWhole EiuentToxicity ApplicationslUnder the National Pollutant Discharge lininstion Program EIPA-S33-R-00-003. US Nviomcntal Protection Agency, Cincinnati, OH 0er8-05.0

1.8 1.6 1 1.4 U z 1.2 t 1.0 V)I 0 -4 0.8 Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaplinia dubia using Moderately Hard Synthetic Water 0.6 I 0.4 0.2._ Test date 7-day IC, = 25% inhibition concentration.- An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC25) Laboratory Warning Limits (mean IC25 +/- S.1 0,I SA.10 0.08) Laboratory Control Limits (mean IC2S b SA.25 SA. 5 a 0.17) USEPA Warning Limits (mean IC25 +/- S,.75, SA.75= 0.45) USEPA Control Limits (mean IC25 + SA., SA 9O= 0.62)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test number Tsdae Control Control Mean Test date Survhial Reproduction (%) (off prinslgfrale) I 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 08-05-03 09-10403 10-07-03 11-04-03 12-04-03 01-06-04 02-03-04 03-03-04 04-06-04 05-04-04 06-08-04 07-07-04 08-10-04 09-14-04 09-14-04 10-05-04 11-02-04 12-07-04 01-11-05 02-08-05 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 33.3 29.3 33.4 31.0 30.4 30.6 30.7 29.0 28.1 32.5 32.9 33.3 27.4 28.7 28.8 29.6 30.5 31.8 31.0 30.0 CT for Control Mean Reproduction (offspringfernale) 31.3 32.0 31.8 31.5 31.3 31.2 31.0 30.6 30.8 31.0 31.2 30.9 30.8 30.6 30.6 30.6 30.6 30.6 30.6 CV CT MSD PMSD cr for Control (%) Reproduction CV (%) (%) for PMSD ('*/) 6.6 4.3 8.0 7.3 9.7 4.4 7.2 7.8 6.2 6.4 6.8 6.3 5.8 4.9 7.5 8.2 8.5 4.9 9.4 6.8 5.5 6.3 6.6 7.2 6.7 6.8 6.9 6.8 6.8 6.8 6.7 6.7 6.5 6.6 6.7 6.8 6.7 6.8 6.8 4.7 3.1 3.2 2.6 3.6 3.5 2.7 3.0 2.8 3.3 3.0 2.6 2.3 2.4 3.0 2.8 2.7 2.5 3.1 2.4 14.1 10.7 9.5 8.3 11.8 11.5 8.8 10.3 10.0 10.1 9.0 7.8 8.6 8.2 10.5 9.6 8.8 7.9 10.0 8.0 12.4 11.4 10.6 10.9 11.0 10.7 10.6 10.6 10.5 10.4 10.2 10.0 9.9 9.9 9.9 9.9 9.8 9.8 9.7 Note: CV - Coefficient of vrdation for control reproduction. On average, the CV for control reproduction is 6.8% in Environmental Testing Solutions, Inc. Ceriodaphnia chronic toxicity tests. Lower CV bound deteninedbyUSEPA(IO" perventile) - 8.9%. Upper CV bound determnined byUSEPA (90 percentile) - 42% MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is arneasure oftestprecision. Thc PMSD is the minimumpercent difference between the control and treatrent tbat can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnio reproduction by 9.7% from the control. LowerPMSD bound determinedbyUSEPA(l0' pesceuble) - 11%. UpperPMSD bound determined byUSEPA (90e perentile) - 37%. CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effuent Toxicity Applications Under the National Pollutant Discbarge Elimination Program. EPA-833-R-00-003. US Envirousnental Protection Agency, Cincinnati, OIL 02-08-0S

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests WU - 3 < CO ,4 IC C " 40 35 30 25 20 15 40 30 20 10 I I I I I I I I I I I I I I I I I I I m A5. 0e..........................................................) Minimum Acceptance Criteri (1.0 offEspring per surviving female) F = %-,.2 = t = -0 C: E M =. >0 w , g

a=-

9 lu = -U 0 E U 4U &.. C5 '2 North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%) ~~~~~~......... I I II I I I I f § g f CI KFx9 S _ o o o Test date a Control Reproduction or Coefficient of Variation (CV) Central Tendency (mean Control Reproduction or CV) Control Limits (mean Control Reproduction or CV I 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 50 45 40 35 30 E 25 L I I I 20 F 15 10 5 0 Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean PMSD) Lower and Upper PMSD Bounds Lower PMSD Bound (10' percentile) - 11%, Upper PMSD Bound (90' percentile) 37% (Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)

Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1 of6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia CdNaCLCR #: 32-Dilution preparation info mation: Comments: NaCI CHM number. CrH Q2O Stock preparation: 100 g NaCVI (dissolve 50 S NaC1 in 500 ml deionized water) Dilution prep (mg/L) 600 00 1000 1200 1400 Stock volume (mL) 9 12 is 8 2 1 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 1500 1500 1506 1500 1500 Test organism information Test information: Organism age: < 24-hours old Randomizing template: LIP Date and times organisms o02-O-0oS OtOb -ro I 1% Incubator number and Z C were born between: shelf location: Organism source: O Zt-o0-3S P% -F YCT batch: 0% *L< Transfer bowl information: pH =7.61SU Temperature =2s3'C Selenastrum batch: jai-o3-S Daily renewal information: Day Date Test Initiation, MHS water Analyst renewal, or batch used termination time 0 Qot%*c4S 11cb3 O2-o~a-oS I c0-o0%eS 0__L__ -cb-oS 2___-_ -tis I 1

02. l O-OS 3

Cz%\\-(CS I11\\ MUZ--Ols : 4 s o2-13-c0s Lbc O-Wt-S A 6 cm--r M 7 OM-lse51os r Control information: Acceptance criteria Summaryv ofst endpoints: % of Male Adults: e7 s 20% 7-day LC50 > NCO % Adults having 3r Broods: I e (Ic l_ 80% NOEC O_ % Mortalit: I s20% LOEC izoo Mean OffspringOFemale: 223.O z 15.0 offspringlfeaale ChV CAS. % CV: I j .8 < 40.0 % 1C25 IcOSa k

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR#: 312 CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I rYoung produced O) C a O) 0

7)

Q)O l Adult mortality 1 U U U l L U U-2 l Young produced T C) 7 1 C O O l Adult mortality \\- _1 t Ll .3 Young produced ' h O O O I.. 0 O _ Adult mortality _ _ U 4 Young produced L_ Lk LA~l Adult-mortality L.- l = = = = S Young produced Z C I I l D I ( ) I I IlC1 I Adult mortality l L_ l 1 lL 6 Young produced .l = = j c j 1 D = 0 L Adult mortality I_ L_ I I l-L - L - L- \\.. 7 Youngproduced l l I 8 J I(D ll5 I 15 1 l8 Total young produced lZ9 l 3 33 l 30 l-Z 7 l 1 l3 32l Z9 l5 3 37 Fin_ L Adult mlort alityv-- l C t_ l i l X tor3' Broods l Cl>C ?el_ > R lConcentration: %AMorality: I U-. l -lMean Offsprine/Female: IDb. 600 mg NaCI/L Survival and Reproduction Data i I __ Replicate numberI l Day l 1 2 l 3 l 4 l 6 l 7 l 8 l 9 l 10 l I Young produced 0r o r c0 r o (Z lolov Ofi l lAdultmortalityl L l L-lL L ILll L I L l l2 lYoung produced l 0 0l 0l 01 - 0 O l 0 Adult mortaliql l tyl L-11lLlQl2 l3 lYoung produced l Cl slt l) l ~ t )lC lOl( llAdult mortality L-l L l-lt l ll l ll l4 lYoung produced l 14 lLA illl ll5 lL l5l5l l l ~Adult mortallty lt _lt 5 Young produced l l l l ~Adult mortality l t l l I l6 jYoung produeed j 0j )jD)j )jOi°i°j( Totalyoungproduced Zz-82.1 3Z Z 31 30 Final Adult Nlortality c

l llL I Concentration:

% Mortality: I 070 Mean Offspring/Female: ,31. C 6 % ReductionNfromaControl: -3S3ri nu

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia CdNaCLCR#: 32-800 mg NaCI/L Survival and Reproduction Data IReplicate number Day l I1 2 3 4 5 6 7 8 9 10 Young produced T p p p ) I O l o o C o I Adult mortality l L- __ E 2 Young produced Q e) n O O) C Adult mortality l 3 Young produced ) r) lC Adult mortatlity =_ 4 Young produced l 4 lA 'l I 'A I Adult mortality L l I L l - l I l S Youngproduced I 1b lO I

1.

I b l I I \\ I Oo I Adult mortality L L L-- L-I 4 a_ l l

=.=

==

= = 6 Young produced J l* O lO l) O o Il il c L Q. I Adult mortality. 1lL-L- \\.- U 7 l I l l 7 l Young produced l l 1-1 IS I l l l l 1 lo ls Total young produced l 22 l32 33 3 'k 29 l31 2a 2 z 31 2l1 Final Adult M ortality l l I § l l l l I l l lConcentration: l Mortality. 7. I Mean Offspring/Female: I2q.a % Reduction from Control: l.1%7z 1000 mg NaCI/L Survival and Reproduction Data l l_____ Replicate number I Day l jI 2-3 7 4 5 6 7 l 8 9 10 lI lYoung produced lO l) C)i l (Z0 nlOl )1 Adult mortality L .~~ L 2 Young produced l Q l Adult=mortality =_ L 3 Young produced l O O l c l J l l ll I Adult mortality l U_ l U. 1l

t.

l il lU .l L l § l4l Young produced l6 LI l qlL L4 I 1 q lt 5 S 9 llAdult mortality l L-l L-L- %. l l ' L-1>r _5_ Young produced O I Z)lI_ Adult mortality lLr L . L-C U. 6 Young produced 4 .a l Adult mortality x-k ll 7 Young produced I l3 l l.3 I LI I to l 1_ YTo ogl youngproduced CZ) -Zs Z S

r.

30

2. lc Final Adult Mortality j

I I I I I I u l I l-Concentration: % Mortality: Mean Offspring/Female:

28. I

% Reduction from Control: l.3

Environmental Testing Solutions, Inc. Page 4 of 6 Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubia CdlfNaCLCR #: .37-1200 m2 NaCUL Survival and Reproduction Data _Replicate number Dav 1 2 3 1 4 S 6 7 8 9 10 Il Young produced O O a O lAdult mortality t L_ %.z 2 Young produced C) C C O O Adult mortality L .L X-- L._ 3 lYoung produced l C)lt O lI: lOlC O Adult mortality 1 4 Young produced f 3 I _ i____ A dult mortal~qIty 1 e 1 1 L 3 Young produced Cl C) I O-J Li 1 0 Adult mortality L -- L I I r i I 6 'Young produced ( 5 CiD l.o lI.l.O.I C) I O3 Adult mortality l L4 Ll I l I l T 1 C I.. 7 Young produced l z l l l Totalyoung produed T °_l T Final Adult Mortality

l l 1t T7 i

c? :? l l LW l I Conentraion: lConcentra in: I % Mortality: l 70 -Mean Offspring/Femalc: l % Reduction from Control: 1 t. 37 I' 1400 mg NaCI/L Survival and Reproduction Data r 7 Replicate number Day 1 1 2 3 4 5 6 7 l S 9 10 I Young produced CI .. J 0 Adult mortality l l Q l L { L l L. L. A-l %-( - l 2 l Young produced l Q l ("I5l ° l n l l ° l Adult mortality ll l3 lYoung produced l0 l () l 0 0 O ° l( 4 Young produced l j l 0 C_ Adult mortality W1'-1 l 1= S Young produced l O 0 O l Il l Ol l Adult mortality] I II l-t i l L I l .= = = Young produced Li _t CI Adult mortality l L LrC. 7 Young produced jC 1zI C C) o3I~ Ttlyoung produced J .L.jO ~ Q I- -L Final Adult Mortality j l tJ l l C +/- l Concentration: % Mortality: 10781 Mean Offspring/Female: I 3. % Reduction from Control: l 1.37

nan I. .lA ~ ~. =

a.

ta hJ-.t.sl~ A* %. Verification of Ceriodaphnia Reproduction Totals Control Day Renpikate number Total 1 21 31 5 6 7 8 19 1 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 6 3 5 4 4 4 5 4 43 5 10 11 9 11I 9 9 10 10 I11 10 100 6 0 0 0 0 0 0 0 0 0 0 0 7 15 15 18 1 3 14 18 15 t15-18 17 Total 293 330 27 27 32 29 1 3 0 600 mg NaClI..-. Day Replicate number Total 1 21 4 5 7 8 9 110 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 .0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 544 54 5 54 46 5 10 9 1 1 1 1 10 1 1 12 10 9 11 104 .6 0 0 0 0 0 0 0 0 0 0 0 7 17 14 15 17 1713 18 17 17 15 16 Total 32 28 31 32 31 29 _34_ 32 31 30 31 800 mg.NaCII Day Replicate number -Total 1 2 3 4 5 161 7 8 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 00 0 0 0 0 3 0 -0 0 0 0 0 0 0 0 0 0 4 4 4 6 4 5 5 4 4 4 4 44 5 10 I11 10 12 8 10 9 9 11I 10 100 6 0 0 0 0 0 0 0 0 0 0 0 7 14 17 17 IS 16 16 15 15 6 1 154 Total 28-32 33 31 29 31 28 28 3 27 298 1000 mg NaCI/L Day Re Ilacate number Total 1 3 4 6 17 8 19 1 0 a a a a 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 3 4 4 4 4 5 4 41 5 8 1 1 9 10 1 0 12 9 9 12 10 10 6 0 0 0 0 0 0 0 0 0 0 0 7 14 1212 1316134 61614 4 Total 27 27 25 26 30 2927 9332 28 1200 mg NaCIIL Day Re liente number Total 1 2 1 3 1 4 5 6 1 7 8 19 110 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 .0 0 0 0 0 0 4 3 2 0 1 0

0.

1 1 0 2 10 5 0 0 2 2 2 2 4 3 5 21 22-6 5 3 0 6 0 0 0 0 3 01 17 7 2 1 5 0 7 0 6 10 0 61 37 Total 10 6 17 9 j9 2 11 1 10 86 1400 mg NaCI/L Day Re Uicate number Total 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 4 1 0 0 0 0 0 1 0 0 0 2 S 0 0 1 0 0 I0 I0 4 0 0 5 6 0 4 0 2 0 0 0 0 6 0 1 7 0 3 6 3 2 0 3 0 0 2 1 Total I1 7 17 15 2 0_ 4 4 6 2 3

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #61 (#32 at 351 Depot St.) Test dates: February 8-15, 2005 Revelwed by: Concentration Replilcte number Survival Average reproduction Coefocat of rest rqdzdO3 ftr. (mgIL NaCI) 1 2 3 4 S 6 7 8 9 10 (%) (offspring/female) nrtath () nafril (%) Control 29 30 33 30 27 27 32 29 31 32 100 30.0 6.8 Not applicable 600 32 28 31 32 31 29 34 32 31 30 100 31.0 5.5 -33 800 28 32 33 31 29 31 28 28 31 27 100 29.8 6.9 0.7 1000 27 27 25 26 30 29 27 29 33 28 100 28.1 8.1 6.3 1200 10 6 7 9 9 2 I 14 8 10 100 8.6 37.3 71.3 1400 1 7 7 5 2 0 4 4 6 2 90 3.8 654 873 Dunnett's MSD value: PMSD2 2.396 8.0 MSD - Minimum Significant Difrerence PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant diff'erence occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Cerlodaphnwla reproduction by 9.7% from the control. Lower PMSD bound determined by USEPA (10* percentile) - 11%. Upper PMSD bound determined by USEPA (90' percentile) - 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnla reproduction in chronic reference toxicant tests. USEPA. 2000 Understanding and Accoundng for MeLhod Vaiability in Whole EMuent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati, 011.

Environmental Testing Solutions, Inc. Statistical Analyses Cerisdspbala Sarvival and Repr uildtim Test-Reproduetion Strt Date 2/22005 Test lD. CdNaC2CR Sampl ED: RtEF-RefToxicant zAl Date. 215/2005 Lab Ui. BT-En*r. Tetling Sot Sample Type: NACL-S4dium eblorkle Sample Date: Protocol PWCR-EPA-121-R-02-013 Tat Speces: CD-CadapbAb dubia Comment Caae-wg/L 1 2 3 4 5 '7 13 D-Conrol1 29.000 30.000 33.000 30.000 27.000 27.000 32.000 29.000 31.000 32.000 600 32.000 2t.000 31.000 32.000 31.000 29.000 34.000 32.000 31.000 30.000 S00 2S.000 32.000 33.000 31.000 29.000 31.000 2t.000 21.000 31.000 27.000 1000 27.000 27.000 25.000 26.000 30.000 29.000 27.000 29.000 33.000 2t.000 1200 10.000 6.000 7.000 9.000 9.000 2.000 11.000 14.000 t.000 10.000 1400 1.000 7.000 7.000 5.000. 2.000 0.000 4.000 4.000 6.000 2.000 Trasferm: Untrassrarmed 1-Tailed 1ee oale Come-mlJL Moss N-Mesa Moas Mbs Mla CV% N 0-slat Crtical MSD Mesa N-Mesa D-Control 30.000 1.0000 30.000 27.000 33.000 6.49 10 30.500 1.0000 600 31.000 1.0333 31.000 2t.000 34.000 5.483 10 -0.954 2.2t7 2.396 30.500 1.0000 t00 29.100 0.9933 29.t00 27.000 33.000 6.259 10 0.191 2.217 2.396 29.t00 0.9770 1000 28.100 0.9367 2S.100 2i.000 33.000 t.124 10 1.t13 2.227 2.396 21.100 0.9213 1200 2.600

0.2867
.600 2.000 14.000 37.258 10 20.421 2.287 2.396
.600 0.2220

'1400 3.800 0.1267 3.E00 0.000 7.000 65.408 10 25.002 2.227 2.396 3.t30 0.1246 Auxillay Teuts Statistic Critied Skew Kell XomoDgrovDTeasxdrfflvsnmmaldbtS tii(p>0.01) 0.56923805 3.035 -0.1081364 0.37941264 Bartlen Test indiues equalivariances (p - 0.52) 4.19896914 15.0262722 Hypothesls Tut (1-tal. O."5) NOEC LOIIC CiV TIr MSDa MSDp MSB MSE F-Prob df Dthnnetf Test 1oo0 1200 1095.44512 2.39625659 0.079S7522 1507.J3667 3.49074074 4.3i-37

5. 54 Tratmentb s D-CaSrol Lineer letorpolatioe (200 Resamplos)

Poiet mgfL SD 95% CL Skew ICOS t97.053124 67.9730995 766.337132 1004.22207 -0.7199 IClo 1006.66667 17.910208 949.652333 1019.21091 -2.6007 ICIS 1022.30769 6.59385625 1009.46726 1034.97577 0.1077 1.0 IC20 1037.94S72 6.26513921 1026.23t1 1050.1543 0.1232 0S IC25 10I3.32974 6.07245975 1042.527t7 1065.30124 0.2460 1C40 1100.51282 6.39075724 1029.43557 1114.41071 0.2904 0.8 1C50 1131.79427 7.2619074 1120.32042 1147.53129 0.2559 0.7 0.6 C0.5 0.3 02 0.1 -0.1 0 o0o 1000 1500 Dose molL Do**.Raspoo&s Plot 5 a 303 a 25 2 F.2 a.20 1i, 1-tall, 0.05 level dsigrificance 'I 02-CS-05

Environxnen6l Testing Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubia CdNaCLCR #: 32-Daily Chemistry: Day Analyst 77f\\[ \\ ~t [A Concentration Parameter pH (S.U.)

1. (

.9.Z DO (mglL) 1 1 - ^8 1 CONTROL Conductivity _la3 (jimrhos/cm) Alkalinity (mg Cal03/L) Hardness (mg Cac 3 L at Temperature q 2.2 l .1 2q.q 2q pH(S.U.) FMZS 1.16 1.21 10 DO (mg/L).7.1

1. ?_

2.0 600 mg NaCi/L Conductivity (izmlos/cm) I$Afr 1o0 Temperature Z54. Z 7 1 25.I .-8 (OC) I pH(S.U.) 1.S5 7.7 .BO 1.10 141 DO (mglL) .81 .9 8 I O 1 .9 800 mg NaCiL Conductivity q D (ijmho /M) 90 Temperature pH (S.U.) t7.82.4 15 ,8 ib -I1 n I I DO(mWL) o 1 _ . 19 0 -I. 8 1000 mg NaCi/L Conductivity (ptmbos/cm) 2V 0 27.6O Temperature 2S.I 2el. 2 ZS I2-SI pH (S.U.) .is8Z 2'(1 .5 8( z 140m alLDO (mg/L) Z7bO 'J. c30 1 0 2Y 1200 mg NaCI/L Conductivity 22z 21ZOD (Vrmhos/cm) Temperature 2S1 2q.2 (C U)~ I., q -'06 DO (mg/L) 17.R Al. -1. l

8.

.18 1400 mngNaCIIL Conductivity 96&02q1 (gmnhos/cm) Temperature 2onucivtyI STOCK Conductivity 100 __________(pmbios/cm) Initial I Final I Initial Final IInitial Final

T0 Environ CI 14 Concentration 1mental Testing Solutions, Inc. -Page 6 of 6 mental Testing Solutions, Inc.

Page 6 of6 Species: Ceriodaphnia dubia CdNaCLCR #:

37-. Day 3 4 5 6 I . I I I Analyst I CAJ I PG) Fct3 I MB r c 5 I CA-1 &5A. I OA j Parameter -4 N CONTROL pH (S.UL) I 7%L-1 7.79 7-.;; T4,9 1.q I -. 9s I t..iL I H fi DO (mg/L) Conductivity (iimhos/cm). Alkalinity (mg CaCO3/L) Hardness (mg CaCO3/L) Temperature (0C) \\14 ~ ZS 7-I-- .t 7.7 Sr7 i 1. t.87 2K8T 3131 105 -4.0 ze-. 2'. 24A.4 zqq 2z4.3 4 4-dl I 4 .5 dl ..I 600 mg NaCi/L Sl., ' pH (S.U.) 7.7tt7 1.6'- I -7.77 I 7.86 I '3.0o I. I - i 9-t DO (mgWL) Conductivity (gmhos/cm) Temperature (CC -7 V Z5.0 2q. 'i I *7.5 - W.1 l 00 I to I I -q.,A I jL.4 2zl.6 pH (S.U.) 1.1 7.7 7.71 8.06 DO (mg/L)

7.

C 7,

7. 7 Of1 Z

13 3

I 800 mg NaCU/L Conductivity 8ii 19l 3 un(mhos/cm)

Temperature 2 ZA.LI 2 1- & 25 2q.S pH (S.U.) 27i.. 7 61 776 7.?o _o6 O I D0(mg/L) 72t 7.1 7.7 BA 1000 mg NaCI/L Conductivity (jimhos/cm) 2&J z_ Z 0 7 oS Temperature Zq.9 Zqq

7.

8S Z. ¶ pH(S.U.) 1.75 7j 7 1.7 7.91 8.o6 S.C. 1 'DO (mg/L)

7.

° I z _f AZ " 1200 mg NaCU/L (Con~d~usctivity a5 ^3 3 ?5?o

2 TTemperature q.f1 q R6 Zl5 zls Zt.8 ZR s0 t

¶ 1400 mg NaCUL CH (S.U) I7 74 7.7Y1 7.q1 R. 07 S.e-l a fiC 1.1-5 DOr(mgL) 1.9 .7 I _ -. i &. 7 I.-___, _ 1 .-5 II 5 w z Conductivity z E aoo INJlc' Temperature (C) 1 25.1 .q. s ZI.8t Zq.7 -zqAJ -9 4.-

4. -

'9-4 9- .9 - Conductivity (umhos/cm) 1z'qCO' ---4<-M I 4-4-4 '9- 411 Initial I Final Initial Final Initial Final Initial Final .1 - - .5 - .5 - a - L 4-

PERMIrTEE NAME/ADDRESS (tnclude Fecf7tyNametocatton ifDiffernt) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _INTEEMM9CE S~? -2S - SODDY - DAISY TN 37384 aciIV_ TV A-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR). (SUBR 01) 0MB No. 2040-0004 I TNO00l 103 G I F-FINAL I PERMIT NUMBER I [DISCHARGE NUMBER I LOW VOL. WASTE TREATMENT POND I lkA^Kll1r^0tl1le_- 0=31ni"k I EFFLUENT I -illi' I WLfflhI4%7_FIflWW.. I4 IYEAR IMO IDAY 1 YEAR IMO DAY NO DISCHARGE F 1*' From 05 02 01 ITo 05 02 _28 NOTE: Read Instructions before completinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE _____________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.1 8.9 12 0 12/28 GRAB MEASUREMENT 00400 1 0 0 PR M

- j

.O SU THE. wGRAB' EFFLUENT GROSS VALUE MROsEMN-W-."

1.

~'~Ek~~- SOLIDS,TOTALSUSPENDED SAMPLE 119 180 26 14 21 19 0 4/28 GRAB MEASUREMENT 00530 1 0 0

.Z-PERMIT-.

LBS/DY *****J 3' L

.K1!

MG/L 7 GRAB_ TREAUIREMENT EFFLUENT GROSS VALUE L MO"AVGM DAILYMXMGD.

4.

AV ,TOAL OIL AND GREASE SAMPLE <42 <43 26 <5 <5 4128 GRAB MEASUREMENT 00556 1 0 0 rPERM T~j 2O~ LBS/DY MG/L ~.~- EFFLUENT GROSS VALUE M0'AG `- -DAIL- .~ 4 O% __WEKLk~GA FLOW, IN CONDUIT OR THRU SAMPLE 1.026 1.281 03 0 28/28 TOTALZ TEATMENT PLANT MEASUREMENT 50050 1 0 0 P~ PER MITYFEOTL EOTY MD .~ ~AL--~

TALZ, EFLETGOS AU

.REO EPORTVG. iDAILYIMX' SAMPLE MEASUREMENT ~REOUIREMENTh i. .1-j;*>i 4 SAMPLE MEASUREMENT PERMI.2?- 4-V." SAMPLE MEASUREMENT ,REOUIREMET -.2 r NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document end all attachments were prepared under My t TELEPHONE DATE direction or supervision In acordance wit a syslen designed to assure thaI qualified personnel 1 ((V J. Randy Douet property gather and evaluate the Information subrnited. Based on my Inquiry of the person or l.A.. persons who manage fe syslem.i or Mtoe persona directly responsible for gatherIng te Principal Environmental Engineer 423 843-6700 05 03 11 informnation, the Information submilitted Is.* to the best of mvy knowledge and belief, true. Site Vice President accurate. and complete. I am aware thal there are significanl penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTED infornmation, Including the possibilityol fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED __________________I________ I______________ CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all etachments here) EPA orm332-1 REV3/99 Prvios eitins mybeuse Pae 1of IF EPA Form 3320.1 (REV 3199) Previous editions maybe used Page I of 1

PERMITTEE NAME/ADDRESS (Include Facr7ity Namelocation ij Different) Name TVA - SEQUOYAH NUCLEAR PLANT ____ Address P.O. BOX 2000 ____________ ____1qTEEOFFIE SB-2A -i SODDY - DAISY TN 37384 Facdity TVA-SEQUOYAH NUCLEAR PLANT T Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN645O 107 F - FINAL I PERMIT NUMBER I DSCHARGE N METAL CLEANING WASTE POND I MONITORING EERIOIQ EFFLUENT Form Approved. OMB No. 2040-0004 From YEAR liiO I DAY I AR lMO I DAY I

- NO DISCHARGE

[]

1 NOTE: Read Instructions before completing this form.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE dlirection or supervision In accordance vwth a sytern designed to assure that qualified personnel A #I 4} ACd I( C 1 J. Randy Douet properly gatler and evaluate the Information submtrted. Based on my Inquiry of the person or persona who manage the systern, or those persons direcily responsible for gathering the Principal Environmental Engineer te V President information. the Infornation submitted is

to the best of mry knoedge and belief, We n

423 843-6700 05 03 1 1 Site Vce President accurate. and complete. Iam aware that there are significant penalties for subndlting false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPED OR PRINTED information, Including the possitbility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT CDEA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) No Discharge this Period EP For 3 - (RE 3_ 9 Prv_ u edtin may beue ae1o EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I

PERMITTEE NAME/ADDRESS (tnc/ude Fec/itiy Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT ____ Address P.O. BOX 2000 ___ LREEROFFICE §8-2?)_ SODDY - DAISY TN 37384_ Facflitv VA - SEOUOYAH NUCLEAR PLANT____ Locafton HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0026450 110 G F-FINAL PERMIT NUMBER ll DISCHARGE NUMBER RECYCLED COOLING WATER I M N EFFLUENT Form Approved. OMi No. 2040-0004 F YEAR l MO lDAY I T MO DAY From 105 1 02 1 01 To 05 02n 1 28

- - NO DISCHARGE [ J **

NOTE: Read instructions before cor NAME/TITLE PRINCIPAL EXECUTIVE OFFICER

1.

J. Randy Douet Site Vlce President I Certify under penalty of law that this document and all attachments were prepared under my direction or supervislon In accordance with a system designed to assure that qualified personnel property gather and evaluate the Information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directty responsible for gathering the information, the information submitted Is, to the best of my knowtedge and belief. true. accurate, and complete. I am aware that there am significant penalties for submitting false information. Including the possibility of fine and Irrpisonment for knoring vidations. A a4fr 4 j s-uc-L Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE OFFICER OR AUTHORIZED AGENT .1' TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference aff attachments here) No Discharge this Period EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I

PERMITTEE NAME/ADDRESS tInClude Facility NameMocation if Different) Name TVA - SEQUOYAI NUCLEAR PLANT Address P.O. BOX 2000 ___ Nt~EROFFICE 5E -2A)_ SODDY - DAISY TN 3784_ faclity_ TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0026450 116 G I F - FINAL I PERMIT NUMBER I DISCHARGE NUMBER IBACKWASH MONITORING zPERIOD lEFFLUENT Form Approved. OMB No. 2040-0004 YEAR l MO 1 DAY i MO DAY i From I OT 02 1 oI T jo9 I T 02 T 28j

^ NO DISCHARGE

= ^^^ NOTE: Read Instructions before completing this form. NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under n y TELEPHONE DATE dlrectlon or supervision In accrdance with a system designed to assure that qualified personnelIJ~ W CA. " DATE J. Randy Douet properly gather and evaluate the Informrsation submitted. Based on my Inquiry of the person or f ) T P persons who manage the systern, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 05 03 11 S~~te \\r~ce President ~ Information, the Information submiritted Is, to the best of myr knowledge and belief, true,___________________ e ice resient accurate. and complete. I am aware that there are significrant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE infonmation. Induding the possibility of fne and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED II CODE Y COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt atachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. _P For _32.1 (RE 3_9 Priu editions.............. ma b. use P.g 1 of 1 EPA Form 3320-1 (REV W99) Previous editions may be used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Locetion f Different) Name TVA - SEQUOYAH NUCLEAR PLANT _ Address P.O. BOX 2000 _QNTEROFFIE SB-LA)_ SODDY - DAISY TN 37384 Facilt TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 117 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH MONITORING PRIOD EFFLUENT Form Approved. OMB No. 2040-0004 Fm To IY YEAR I MO I DAy I From 105 1 02 -101 -1To 0 l02 1 28 1 NO DISCHARGE W:1... NOTE: Read Instructions before comPletinq this form. I NAME/rITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under mry AI I TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather and evaluate the Information subnitted. Based on my Inquiry of the person or AA .i4J-L A o CL1 persons who manage the system6 or those persons directly responsible for gathering thrniaenvrnetlEg~er 43 83-70 0 3 1 S i information the Information aubmitted is

to the best of my knowiedge and Wief, true, Princpal Envirnmental Engineer 423 843-6700 05 03 11 ite Vice President accurate, and comprete. I am aware that there are significanl penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE L

Information, Including the possibilityof fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA l NUMBER YEAR MO DAY TYPED OR PRINTED OD COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a attachments here) Operations performs visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1

- Elba 0 l cc~%WS sr I MUUc ~linciuoet -aclit V Name/Locatton it Dlfferent) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ___ lfqERQFF9CE qSZ -2) SODDY -DAISY TN 37384 FaciLty_ TVA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NrA1UNALPlVLLUIANI DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) OMB No. (SUBR 01) 0MB No. 2040-0004 l TNO026450 118 G F-FINAL I PERMIT NUMBER [ I DISCHARGE NUMBER WASTEWATER & STORM WATER RMrKltI2I%0NIr: 01=0nrln I EFFLUENT I I-- YEAR IMO YEAR NO DISCHARGE NOTE: Read Instructions before completinq this form. PARAMETER \\ QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE ______________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSI OXYGEN, DISSOLVED (DO) SAMPLE 19 MEASUREMENT 00300 1 0 0 =MGT

- - - - ./.<

G m

EFFLUENT GROSS VALUE DEAREETI~, GIL ~TWIEK GAB SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 0 MGL TWICE/. ,GRAB, EFFLUENT GROS VAU EURMENT.'>... ~ ________'DAILY X WE SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 0 A s -*O i MLIL .ONCE -GRAB mm EFFLUENT GROSSVLETEURMN,{~. SS VALUE .~ A1 -X~ -~ MONTH-, FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 e PERMIT REPORT. REPORT MGD c..t

ONCE STIMA FR OEUI REMEN T MO AVG GDAILY MX 4-i D.

EFFLUENT GROS VAU4 2Ž<--1.-.~*.BTH SAMPLE MEASUREMENT ..REUIRE.MENTm - SAMPLE MEASUREMENT ,-PERMIT---~ t X -REQUIREMEN SAMPLE MEASUREMENT {E-PERMJTr-e#SaX NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and ON attachments were prepared under my I /ITELEPHONE DATE dJrection or supervision In accordance with a system designed to assure that qualified personnel lliiOr J. Randy Douet property gather and evaluate the Informatlon submitted. Based on my Inquiry of the person or persons who manage the system. or those persons directly responsible for ganteing the Principal Environmental Engineer 423 843-6700 05 03 11 StViePeietInformation. the information submnitted Is,* to the beat of my knowledge and belief. true. 423___843__6700_______03______ Site Vice President accurate. and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYERPITDinformatlon. Including the possibility of fine and Imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUM13ER YEAR MO DAY TYPED OR PRINTED_ CODE Y COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference eff ettachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EP For 3320.1 (RE 3_9 Previous edtin mab use P.g 1,. of 1 EPA Form 3320-1 (REV 3/99) Previous editions maybe used Page 1 of 1}}

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