Text

Discharge Monitoring Report for November 2004
	ML043510058
Person / Time
Site:	Sequoyah
Issue date:	12/13/2004
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML043510058 (114)
	v • d • e

Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 December 13, 2004 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR NOVEMBER 2004 Enclosed is the November 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely,

)A phcm4U CAXLLC'uca/LC Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):

Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 -r§ 3?62 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEE NAMEIADDRESS (Indude Faci7ity Name4locafion iDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT __ (SUBR 01)

Address P. OX 2000

_ _ _ _ffTEROFMCE S -2S - - -_ I TN026450 101 G F - FINAL SODY. DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE fgi!LNy_ TVA - SEQUOYAH NUCLEAR PLANT _

Location HAMILTN COUNTY --------- i W~nIT^IMoAnolnr1 IV-.I1-Ir~'ItI I

I EFFLUENT From MO DAY Toh I O DAY - NO DISCHARGE E -

ATTN: Stephanie A. Howard urom0411 I To 04 -I 1 30 NOTE: Read Instnictlons before comDletina this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

_______EX OF TYPE AVERAGE MAXIMUM UN US MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******** 21.6 04 0 30/30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 hPERMrIf.;, jt. &C~ee -, 4 e****

~ ~ 30 ~DE C SE -REe INSTREAM MONITORING __ _ _ _ _ _ _ _ _ _~*

_EPERMREMEITm D iLYMX~ _ __ .PE MT.

TEMPERATURE, WATER DEG. SAMPLE ***** ** 35.3 04 0 30/30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 0 i_

_ cSE' -REQ,.

EFFLUENT GROSS VALUE** __________________ ******** ~ **

DAIL MX D C. 0Y' PPE 4S30MIT, CREB A :

PH SAMPLE i0 7.22RAB 7.5 12 8ee37 MEASUREMENT 12 0 8/30 GRAB 00400 1 0 0 ESu WEEKLY. ,iiGRAB'.

003 0*;REQUREMErNT..

TAEOUIREMENNTT 3 S . ~.. 6 .".. . *-.oo¢MLt EKL

'MXMJIJ EFFLUENT GROSS VALUE ~ ,~~.~~~MNMM SOLIDS, TOTAL SUSPENDED SAMPLE ******* ** 5 6 19 0 4/30 GRAB MEASUREMENT 00530 1 0 0 PERMIT., i;',, e2~0~jA .**eeIi..**e* ~ O~ MGI1. WEEKLY f~~GRAB~l EFFLUENT GROSS VALUE ~ ~ "~ ~;~ OVg~AL.X OIL AND GREASE SAMPLE <5 <5 19 0 4130 GRAB MEASUREMENT 1 00556 1 0 0 _7,`PERMIT,~f~ "~~~'~" 5 0~ GL~ EKY RAB EFFLUENT GROSS VALUE . r-AOV~

.~EURMN 4 AL X ____.L FLOW, IN CONDUIT OR THRU SAMPLE 1490 03 0 30/30 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 0 ,O1E"r EOT~MD . ~ ~ ** ~ -- CONTINE COD 506 .. PERMI4X< rGD -,. 0 3 6 CORDReee*...

'REQUIREN --- , 0O5' A MGL WEK.CCT DAS .

EFFLUENT GROSS VALUE ,, I

______ ___ _____ . ' NT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I.Ce=fy= d=r pe=a=ty of lw ttthidoaaeidsltmentsweeprepd MY

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J. Randy Douet operty a erad evaluate the I mation submitted, ased on my Inquiry of the person or pefsons who manage thie syste or those persons directy responsible for gatherifg the Information, the Information submitted Is . to the best of my tnowledge and belief. true, PincJipal ioumntalict Site Vice President accuate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTM information, the possibility of fne nd imprisrnent for indUding wing violations.

OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED I _ _ _ _ _ _ _ _ _ _ _ _ _ _

A ________ _________

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)

No dosed mode operation. The following injections occured: 1. PCL-222 (max. caic. conc. was 0.049mg/L-limit 0.100mgIL) 2. PCL-222[PCL-401 (max. cafe. conc. was 0.030mglL-limit 0.160mgIL) 3. H-130M (max. cabc. conc. was 0.043mg/L-limit 0.050mg/L) 4. H-130M (low detection level analytical method was <0.020mg/L-limit 0.050mg/L))

enRr or m 3 7 u. v t op B a wA o a_e. :a crE .... ons. m_ ay _ D_ P 1o tt-A rOrMl JJZU-1 {REV JWIVV) Previous eUiMonS may De used PageI of 2

PERMITTEE NAMEIADDRESS (Incfude Facilit NamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT ___ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Address P.O. BOX 2000 ____________ (SUBR 01)

____f1lNE~ROF9lCE SS3______ I TN026450 ll101 G F-FINAL SODDY - DAISY TN 37384 I PERMIT NUMBER . l ISCHARGE NUMBER DIFFUSER DISCHARGE ady_ _MA -%SEQUOYAHNNUCLEAR PLANT _---

Location HAMILTON COUNTY __________ IMNITORING PERIOUD l .EFFLUENT ATTN: Stephanie A. Howard YEARrMO I DAY I YEAR MO DAY

- NO DISCHARGE I ...

NOTE: Read Instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE ****** *

0.2 62 0 30/30 CALCTD CHANGE MEASUREMENT 82234 1 0 0 .PERMIT ;rY_ 2;0.,* DEG C S CONTIN CALCTD EFFLUENT GROSS VALUE EQUIRE IHR .UU' "

TEMP. DIFF. BETWEEN SAMP. & SAMPLE ******** 1.3 04 0 30130 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 W 0 t"'PERMIT7. 4wl*! 5 DEG. C. - .CONTIN CALCTD BORON, TOTAL SAMPLE *******5****** <0.2 <0.2 <0.2 19 0 1/130 GRAB MEASUREMENT EFFLUENT GROSS VALUE __,S MGL . , O. N. CE.,.

SAMPLE MEASUREMENT

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SAMPLE MEASUREMENT

,;PERMI.p. .. .

EFFLUENT GROSS VALUE MONTH__

SAMPLE MEASUREMENT SAMPLE MEASUREMENT

_________________________N,'t -- - --- __ __ L,. W--- " * --- ,. 5. .* ,

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under panatty of law that this doaent and all attadimets were prepared undetr my Erectionor supervision si accrdne wit a sstem designed toassse that quahfiedpersonrsil }^

r 1 Ltf7))CZ(C i TELEPHONE Zrnn.,R4Z]c DATE J. Randy Douet properly gather and evaluate the Infonnation submitted. Based on mny Inquiry of the personl or Ujrlf w lPres e acIdentscaste. es.J womaae end complee. Iam aware that hereeere significant m penaltes w.dead for submitingn.P.npl.nioneta falae SIGNATURE OF PRINCIPAL EXECUTIVEf ngne 43.e......eb.lo

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84 .70 4 2 1 TYERNTDrfonnafon.

O Incuding thet possibtility of fine end Impusonment forkno'g violations. OFFICER OR AUTHORIZED AGENT 1AREA lNUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refference all attachments here)

CCW data for November 2004 is attached.

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3199) Previous editions may be used Page 2 of 2

CCW TRENCH DatelTime Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 11/01/2004 1230 <0.5 mglL 11/02/2004 1416 CVS EPA8015B CCW CHANNEL DaterTimeCollected Extractable PetroleumHydrocarbons Analysis Date/Time Analyst Method 11/01/2004 1225 <0.5 mg/L 11/02/2004 1339 CVS EPA 8015B

PERMITTEE NAMEIADDRESS (include Facility Namelocaton if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MA.OR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT________ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000

_ _ __lN EROFFICE S3- ___ _ l TNOOZ6450 101 T F -- FINAL SODDY -DAISY TN 37384 _______ I PERMIT NUMBER I [DISCHARGE NUMBERl BIOMONITORING FOR OUTFALL 101 FacIjy TVA .SEQUOYAH NUCLEAR_ LANT ____

Locaon _AMILTON COUNTY __________ I I

RAnk11t ^1nd1r

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Mc~Dinn WAr'.IXLSI I, EFFLUENT From YEAR IMO 04 11 I1 1 DAY YEAR I O DAY I*** NO DISCHARGE [7 -

ATTN: Stephanie A. Howard To 04 111 30 NOTE: Read Instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION No. FREQUENCY SAMPLE

__ ____ ____ ____ ___ _ __ __ __ __ _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100 23 0 110 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 RM:; tMIN' . PERCENT C OMO

, SI EFFLUENT GROSS VALUE r,' M lM .M'~,.~ .

IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1/0 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMT < -' * -

X PERCENT -_QTRLY_ COMPOS EFFLUENT GROSS VALUE 2'EUREEJ 24 , .... ..- l ,--t SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT

, ,.EQUIREMENt . , _

SAMPLE MEASUREMENT

,REQUIREMEhTi' 5 -t.

SAMPLE MEASUREMENT

REOUIREMENT- .W NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify wuder penalty of law that this docunent and all antadcments were prepared under my.

J. Randy Douet President direcion or supervision hI accordance with a system designed to assue that qualified perso4el properly gather and evaluateIhe Information subintted. Based on my inquiry of the person or persons who mnanage the systemn or -hose prsnons dirtrety responsibbe for riathiig informatIon ihe Information submitted Is, tothe best of my lrno.medge and belief, true.

the ASI WLIU Prnia E4 niomna

. 4nWM8 .

nine 2 TELEPHONE 4-70 0 DATE 1

I.r Site Vie accurate, and complete. Iam awsre that there ea significant penaltles for subnitting false SIGNATURE OF PRINCIPAL EXECUTIVE WPiformation Including the possibility of fine and Inprisonment for knowing violatIons. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _ _ECODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all atachments here)

Toxicity was sampled November 7-12, 2004. Report Is attached.

EPA Form 3320-1 (REV 3199) Previouseditions maybe used Page 1 of I

December 8, 2004 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, NOVEMBER, 2004 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected November 7-12, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.

Call me at (256) 386-2755 if you have any questions or comments following your review of the report.

eq'w")- '~LkLLV Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):

Files, R&TA, CTR IB-M SQN November 2004M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: December 8, 2004

1. Facility / Discharger: Seguovah Nuclear Plant/TVA

2. County/ State: Hamilton/Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1531.8

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQ10: 3491.1

8. Outfall Tested: 101

9. Dates Sampled: November 7-12. 2004

10. Average Flow on Days Sampled (MGD): 860, 870, 873, 874, 868. 877

11. Pertinent Site Conditions: H-130M was injected from November 9-12, 2004. The dates and times for the H-130M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period.

Injection Location Date/Start Time Date/Ending Time (ET) (ET)

Essential Raw Cooling Water (ERCW) 11/09/2004 1000 11/12/2004 1800 Train A

12. Test Dates: November 9-16. 2004

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Danhnids (Ceriodaphniadubia)

15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Intake: 100.0 Pirnephalespromelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC.5 = 43.9%

17. Test Results: Outfall 101: Piniephalespromelas:IC2 5 > 100%

Ceriodaphniadubia: 5 > 100%

LV treated Outfall 101: Pirnephalesprornelas:25> 100%

18. Facility

Contact:

Stephanie Howard Phone #: (423) 843-6700

19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected November 7-12, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.

2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date . Date (MMJDD[YY)/ (MM/DD/YY)/ Arrival Initial (MI/DD/YY)/

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (OC) (mg/L) Used By 101 11/07/04 0830 to 11/08/04 1350 2.4t <0.10 11/09/04 1340 Intake 11/08/04 0701 11/08/04 1350 3.5 <0.10 11/10/04 1307 101 11/09/04 0846 to 1337 11/10/04 1.4t <0.I0 11/11/04 1252 11/10/04 0746 1/0413711/12/04 1400 Intake 11/09/04 0816to 11/10/04 1337 1.1 . <0.10 11/11/04 1252 11/10/04 0716 11/12/04 1400 11 11/1 1/04 0757 to 1/20125 2.1 <.1 11/13/04 1332 11 11/12/04 0657 1120125 1.4 <.0 11/14/04 1326

____ ___ _ _ ___ ____ ___ __ ___ 11/15/04 1350 11/13/04 1332 Intake 11/11/04 0729to 11/12/04 1225 1.0 <0.10 11/14/04 1326 11/12/04 06291150430

T`RC = Total Residual Chlorne tSamples were collected in two 2.5 gallon cubitianers. Temperature was measured in each cubitianers upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.00C0 in a warm water bath.

AliQuots of Outfall 101 and Intake samples were UV-treated through a 40-watt SmartUV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes.

3

Pimephalespromelas Ceriodaphniadubia Test Organisms:

1. Source: Aquatic BioSystems. Inc. In-house Cultures

2. Age: 22.0-23.5 hours5.787037e-5 days 0.00139 hours 8.267196e-6 weeks 1.9025e-6 months old <24-hours old Test Method Summary:

1. Test Conditions: Static. Renewal Static, Renewal

2. Test Duration: 7 davs Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic Water Water

4. Number of Replicates: 4 10

5. Organisms per Replicate: 10 1

6. Test Initiation: (Date/Time)

Outfall 101 11/09/04 - 1322 ET 11/09/04- 1340ET UV Treated Outfall 101 11/09/04- 1310ET

7. Test Termination: (Date/Time)

Outfall 101 11/16/04- 1300ET 11/16/04- 1253 ET UV Treated Outfall 101 11/16/04 - 1318 ET

8. Test Temperature: Outfall 101: Mean = 24.7 0C Mean = 25.0C (24.2 - 25.4 0C) (24.5 - 25.3 0C)

Test Temperature: UV-Treated Outfall 101: Mean = 24.90 C (24.2 - 25.40C)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Softvare, McKinnewville. CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephalespromelas Chronic/ 7-day Toxicitv Test.

(Genus species) (Type / Duration)

Conducted November 9-16. 2004 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 .100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(% Effluent) (replicate numbeM 12 3 4 Mean Control 0.571 0.556 0.618 0.546 0.573 10.98% 0.661 0.669 0.694 0.599 0.656 22.0% 0.658 0.520 0.533 0.600 0.578 43.9% 0.696 0.655 0.658 0.623 0.658 72.0% 0.626 0.635 0.607 0.525 0.598 100.0% 0.600 0.558 0.582 0.638 0.595 Intake 0.629 0.644 0.662 0.606 0.635 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.9%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = I 00/LCso: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted November 9-16, 2004 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions2 3 4 67

(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

(%Effluent) T(replicate number)

I 3 4 5-_ 6 7 8 9 10 Mean Control 28 27 33 27 29 30 29 31 31 31 29.6 10.98% 34 31 33 34 32 31 28 27 29 31 31.0 22.0% 34 32 33 33 33 36 27 29 33 29 31.9 43.9% 32 31 34 34 32 34 35 31 30 32 32.5 72.0% 38 33 37 33 33 35 37 32 34 35 34.7 100.0% 30 34 35 31 36 33 30 35 31 36 33.1 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.9%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 1OO/LC 50 : TUc = 100/ IC2 5 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted November 9-16. 2004 using water from Intake Percent Surviving Test (time interval used - days)

Solutions 1 2 3 4 5 6 7

(% Effluent I Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#younglfemale/7 days)

(% Effluent) Daa (replicate number)

I1 2 3 4 5 6 7 l8_ 9 Mean Control 29 34 27 29 28 29 29 26 J 32 30 29.3 Intake 28 29 29 28 25 27 1272 8 31 28.0 IC 2 5 Value: >100% Calculated TU Estimates: <1.0 TUc*

Pennit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = I00/LC5 0: TUc = 100/ IC25 7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimegphales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted November 9-16, 2004 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 98 98 98 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(% Effluent) (replicate numer)

__ _ __ _ _ 1 2 3 4 Mean Control 0.613 0.686 0.677 0.569 0.636 10.98% 0.685 0.617 0.597 0.679 0.645 22.0% 0.604 0.679 0.649 0.652 0.646 43.9% 0.516 0.514 0.666 0.668 0.591 72.0% 0.592 0.639 0.666 0.715 0.653 100.0% 0.715 0.611 0.560 0.754 0.660 Intake 0.637 0.694 0.734 0.661 0.682 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = I 00/LCSo: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25)

Pimephalespromelas I November 9-16, 2004 1238 7-days KC1 0.54 g/L Ceriodaphniadubia November 2-9, 2004 1230 7-da s NaCI 1.06 g/L 8

PHlYSICAICIIEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Pintephales promelas and Ceriodapliniadutbin Tests, Sequoyah Nuclear Plant Emuent (SQN), Outfall 101, November 9 -16, 2004.

Test Sample ID Temperature (C) Dissolved Oxygen (mglL) pit S.U.) Conductance Alkalinity llardness Total Residual Initial Final Initial Final Initial Final (tmhos/cm) (mg/L CnCO1) (mg/L CaCO,) Chlorine (mg/L)

Control 24.9 24.6 7.9 7.5 8.05 7.86 298 62 85 Control 24.7 - 25.3 24.3 - 24.9 7.8 - 8.0 6.9 8.0 7.98 - 8.12 7.72 7.96 293 - 306 61 - 63 83 - 89 1098% 24.9 24.5 7.9 7.5 8.08 7.83 285 10.98. 24.7 - 25.3 24.3 - 24.8 7.8 - 8.0 6.9 - 7.9 7.99 - 8.15 7.65 - 7.94 280 - 290 - -

22% 24.9 24.5 8.0 7.5 8.08 7.82 270

_ 24.8 - 25.1 24.2 - 24.7 7.7 - 8.2 6.8 8.0 8.02 - 8.13 7.66 . 7.94 262 - 283 - .

43.9% 24.9 24.4 8.0 7.4 8.05 7.80 233

__._. 24.6 - 25.2 24.2 - 24.7 7.7 - 8.2 6.8 - 8.0 8.01 - 8.09 7.66 - 7.93 225 - 238 72% 24.9 24.5 8.0 7.4 8.01 7.78 189

.E 24.5 - 25.3 24.2 - 24.7 7.7 - 8.2 6.9 - 8.0 7.97 - 8.04 7.64 - 7.91 181

194 - -

i 100% 24.9 24.5 7.9 7.4 7.96 7.76 145 52 57 <0.10 24.4 - 25.4 24.3 - 24.9 7.7 - 8.2 7.0 - 7.9 7.92 - 8.01 7.62 - 7.86 139 - 150 51 - 53 54 - 59 <0.10 - <0.10 Intake 25.0 24.5 7.9 7.5 7.99 7.76 142 SI 57 <0.10 24.7 - 25.3 24.3 - 24.7 7.8 - 8.2 7.1 - 8.0 7.93 - 8.03 7.62 - 7.86 135 - 146 50 - 51 54 - 59 <0.10 - <0.10 Control 25.0 25.0 7.9 7.9 8.05 8.00 298 62 85 24.7 - 25.2 24.8 - 25.2 7.8 - 8.0 7.8 . 8.1 7.98 - 8.12 7.89 - 8.08 293 - 306 61 - 63 83 . 89 10.98% 24.9 25.0 7.9 7.9 8.08 8.01 285 . .

2.% 24.7 - 25.3 24.7 - 25.3 7.8 - 8.0 7.7 - 8.2 7.99 - 8.15 7.94 - 8.07 280 - 290 . .

24.9 24.9 8.0 7.9 8.08 8.01 270 2 24.7 - 25.1 24.7 - 25.1 7.7 - 8.2 7.7 - 8.2 8.02 - 8.13 7.95 --8.07 262 - 283 24.9 24.9 8.0 7.9 8.05 8.01 233 .

24.8 - 25.3 24.7 - 25.1 7.7 - 8.2 7.8 - 8.2 8.01 - 8.09 7.94 - 8.06 225 - 238 72% 24.9 25.0 8.0 8.0 8.01 7.99 189

,_ 24.6 - 25.3 24.8 - 25.3 7.7 - 8.2 7.8 - 8.2 7.97 - 8.04 7.91 - 8.05 181 - 194 100% 25.0 25.0 7.9 7.9 7.96 7.96 145 52 57 <0.10 24.5 - 25.2 24.7 - 25.3 7.7 - 8.2 7.7 - 8.2 7.92 - 8.01 7.88 - 8.03 139 - 150 51 - 53 54 - 59 <0.10 - <0.10 Intake 24.9 25.0 7.9 7.9 7.99 7.93 142 SI 57 <0.10 24.5 - 25.2 24.7 - 25.2 7.8 - 8.2 7.7 - 8.1 7.93 - 8.03 7.86 - 8.01 135 - 146 50 - 51 54 - 59 <0.10 - <0.10 Overall temperature ('C) Average Minimum Maximum Pimephales promelas 24.7 24.2 25.4 Ceriodaphnia dubia 25.0 24.5 25.3 9

PHIYSICAL/CIIEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pihnepihalesprornelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, November 9-16, 2004.

Test Samplc ID Temperature CC) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Initial Final Initial Final Initial Final (Amhos/cm)

Control 24.9 24.7 7.9 7.5 8.09 7.82 302 Control 24.8 - 25.3 24.3 - 25.1 7.8 - 8.1 6.9 - 8.1 8.04 - 8.15 7.66 - 7.95 285 - 310 10.98% 25.0 24.7 7.9 7.5 8.08 7.80 284 24.7 - 25.3 24.3 - 25.0 7.7 - 8.2 7.0 - 8.1 8.01 - 8.12 7.66 - 7.96 269 - 295 l; 25.0 24.6 7.9 7.5 8.07 7.80 270 z 22% 24.7 - 25.4 24.2 - 24.9 7.7 - 8.2 6.9 - 8.2 7.99 - 8.12 7.64 - 7.96 262 - 284 I..

43.9 ° 25.0 24.7 7.9 7.5 8.05 7.78 237

,U.__ 24.8 - 25.3 24.3 - 24.9 7.7 - 8.2 6.9 - 8.2 7.98 - 8.12 7.63 - 7.94 233 - 240 72% 25.1 24.6 7.8 7.4 8.01 7.75 192

_ 24.8 - 25.4 24.2 - 24.9 7.5 - 8.2 6.8 - 8.2 7.94 - 8.05 7.59 - 7.92 187 - 194 100% 25.1 24.8 7.8 7.4 7.97 7.76 146 100% 24.7 - 25.4 24.4 - 24.9 7.6 - 8.2 7.1 - 8.1 7.90 - 8.02 7.64 -' 7.88 139 - 152 Intake 25.0 24.7 7.9 7.3 7.99 7.73 145

_ 24.9 - 25.2 24.2 - 24.9 7.7 - 8.2 7.0 - 8.1 7.92 - 8.08 7.57 - 7.89 141 - 149 Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.9 24.2 25.4 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected November 7-12, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.

Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.

11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephalespromelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.

2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimepihalespromelas None

2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephalespromelas None

2. Ceriodaphniadubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360.1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8. Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by EPA Method 330.5.

OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturinglacquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2 5 values in mg[L KCI or NaCI.

2. Standard Toxicant: Potassium Chloride (KC] crystalline) for Pimephalespromelas.

Sodium Chloride (NaCI crystalline) for Ceriodaphniadubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring November 7-12, 2004 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 12, 2004 2

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 12, 2004 3

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 12, 2004

.. Sodium , Towerbrom ..PCL-222~ PCL-401 CL-363 H-130M

. Hypochionte mgfL mg/L PF ;mg/L'-

...mgL I '-Phosphate,. Copolymer .DMAD;. mgJL Quat,..

TRC 4 >;~ I TRC

-J t .'. .:.':- S.

Azole !6.Z" -, "'

-4 4 4 4 9 01/02/2002 - - - , :' :

< 0.0079 .. 0.023 -~ 0.02 -'0.0061;r 01/03/2002 (*. .... .

< 0.0042 023.023- 0.014 01/0412002 0.0124 0.014 ' 0.009 01/05/2002 < 0.0042 - ...0..

01/0612002 < 0.0042 .i . . Z 01/07/2002 < 0.0089 4 0.024 ; _-

0.014 .I0. 006_ _

_ = _

02/24/2002 < 0.004 0 .0 .

4..

02/25/2002 r' '-! . I 10 1

< 0.004 0.023 k: 0.023 . - I.I. ..

. : '4<@

_j it, i, 1 r, "c, ; An Aft 02/26/2002 0.0143 f ,,0;007-I-.-

02/27/2002 < 0.0041 m.'I 02/28/2002 < 0.0041 03/01/2002 < 0.0041 =

05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/0912002 I 05/10/2002 =

08/04/2(

108/05/2(

=

4

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 12, 2004 I

I 5

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 - November 12, 2004 6

Sequoyah Nuclear Plant Biomonitoring November 9-16, 2004 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of *1 I Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier 28801 General Comments:

Facility Sampled: Sequoyah NP

- 4~40OJA& *4~. &4-4~

Phone: 828-350-9364 4 t zc l C.-6 4 CV = 0 -

NPDES Number: TN0026450 d# l6*-.

Collected By: Wanda Allen Fax: 828-350-9368 Field Identification/ GrabComp. Container Flow 5.

Sample ription N MGD nEvenuet .v Volume ) (Mark as Appropriate).5.

Collected - .

Data Time Yes if Yes, No Trce -> g -D ,.5fAe.

2 (2.5 gal) N SQN-101-TOX Comp 11/7/04-11/8/04 OF3/ 2 (2.5gal.NA SQN-INT-TOX Comp 11/7/04-11/8/04 0(0) 1 (2.5 gal) NA - -

Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Wanda Allen 11/8/2004 Express Courier 11/8/2004 Express; g__t__

} rj D q

__ _god_

\ 2 X11/8/2004

.t/U/3SO TS11/8/2004 l35 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 2_ of 3_

Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments:

NPDES Number: TN0026450 Phone: 828-350-9364 do

_ro t' Collected By: Wanda Allen Fax: 828-350-9368 Field Identification I Grab/Corn Collection Date/Time Container l N.-.... ,..

Sample Description P. Numbr & MOD Rainh Event? cru. Act Volume (Mark as Appropriate) .f.. . ,. ..- g

_________ Collected a1j e YsDaT if Yes, No Trace ltk t ' . m SQN-101-TOX Comp 11/9/04-11/10/04 ° 2 (2.5gal) NA . .....I SQN-INT-TOX Comp 11/9104 -11/10/04 1 (2.5 gal) NA .. =- ;7 d 4E Sample Custody -Fill In From Top Down _

Relinquished By (Signature): Date/Time . Reccived By (Signature): Date/Time Wanda Allen 11/10/2004 Express Courier 11/10/2004 Express Courier 11/10/2004 ' TETs 11/10/2004

, .( .__

istructions: Clients should fill in all areas except those in the "laboratory Use" block Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page of q Delivered By (Circle One):

Client: TVA Environmental Testing Solution, Inc.

351 Depot Street FedEx UPS Bus Client Project Name: Sequoyah NP Toxicity P.O. Number N/A Ashville, NC Olher (specify): Express Courier General Comments:

Facility Sampled: Sequoyah NP 28801 NPDES Number: TN0026450 Phone: 828-350-9364 ,T- -o6 8O77077 v)ua 70 Fax: 828-350-9368 W9/A/ 6M0 ois/Ar5- po-Collected By: Wanda Allen F6Ve6 D071VI6 ,

A-/l/ we, d/

Field Identification/ Grab/Comp Collection DaterTune Container Flow jV( ~ &S1 '

Sample Description Number & (MGD) Rain Event?

Volume (Mark as Appropriate)

Collected > ...............

DIft TY Yes - ml No Truce KrTtmvl SQN-101-TOX Comp 11/11/04 - 11/12/04 2 (2.5gal) NA SQN-INT-TOX Comp 11/11104 -11/12104 07tt 1 (2.5 gal) NA jiiz Sample Custody - Fill In From Top Down CuJoA,4o tA . oSk H.i Date/Time Received By (Signature): Date/Tim ) l Relinquished By (Signature):

Wanda Allen 11/12/2004 Express Courierl/1212004 Express Courier 11l/2/2004 15l ETS 11/12/2004 each Instructions: Clients should fill in all areas except those in the '¶Laboratory Use" block Biimon6itoring samples arc preserved by storing them at 60C and shipping them in ice. The hold time for sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.

Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testinp-Solutions. PagelI of 6 i~~nvironmental~~~~~ ouin.Ic Inc. _etn ae1o Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project #: I 554 Dilutionpreparation inforrmation: Comments:

Dilution prep (No) 10.98 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:

Organism age: 22 2 3.S0%be Randomizing template: rCee, Date and times organisms 11-o-0A iNM4 -M s5 Incubator number:

were bom between: 3_

Organism source: AEG &WER 1n-aCA Artemia lot number: f&IWSS Transfer bowl information: pH = Temperature = °C Total drying time:

24.0 Date /Time in: it-tWA IS2D Average transfer volume: 4 Date /Time out: IVti-U l Oven temperature: (,_,C.

Dailyfeeding and renewal information:

Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time M H5 0 L 11L9q *t NCO bi I3 II-O4 Oqgip) l I . Az 3 I- 11 y 0850 I Is -l\-C.-o4 jo2 &4 1ba0-?.o-I.o A 4 ___ 1i.I3-Oq OciOt I Z3003 %I-2CA+/-. 04 .1q1Z.01t /.0fr7&

s II- I 4 oq 0tI5 -I-- l2q 0 It- 1LT-oq CY 1112z. ol[ o-L 6 Il. tS42 &S5o V~Q tZ32- Oq 0O11121oi J-oz KE K 7 1 *O. t, 1X Controlinformation: Acceptance criteria lSummaryof test endpoints:

% Mortality: All. s 20% 7-day LC30 10 I>

Average weight per initial larvae: O.513 0 I NOEC /tO%

Average weight per surviving larvae: l 0.53 2 0.25 mgnarvae LOEC > Ic)'fo ChV > Iffla IChV > I MN7

E;nvironmental Testine7Solutions. Inc. Page 2 of 6 Environmental Testing Solutions. Inc. Page 2 of 6 Species: Pimephalespromelas Date: / l -vq-L)4 Client: TVA I Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 10.98% - 22%

A B C D E F G H I S I L 0

Ib j

___ __ /0 /0 10 /0 Io /0 /0 ) Ic IC)___IC)01010 I) o/0)I/0 /0 / /0io'o0 2 1D 10 1 / /0 I O0 10 I0 /0 1o 1t /0 3_

_ _ /0 ID /0 /0 l1D /0/0 /0_/0/

4

___ I lb 1 ) lb (b 16 10 16

/0 l0 ID 4 1 10 10 10 I10 D /I 10 6 /0 /D /t)0 IC l0 10 10 I0 IC) I J 10 /0 710 l __/0(l / _ / /l l _

A-Pan weigt (mg)_

Tray color code:: gowv ~d.. a.

Analyst: tA ,uJ i do.' ° \' _ _ (V %

B - Pan + Larvae weight Analyst: J 4,O \1'4 - L0 Larvae weight (mg) - A - B

_~ _D 64 tts? t N_ s _0 V \V b 6# i Weight per Initial number orlarvac (mg)

- C / Initial number of larvae 4 0- O,;o 0-O0 tI, o0 O0

'I 0*

V0 oibP O-0ou 43 forb~

Average Percent weight per reduction Initial from control 0 S B - S 06. 519-- ocU number of (%/)

lanae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k - killed, m = missing, sk = sick, sm = unusually small, lg = unusually large, d&r = decanted and returned, w = wounded. .

Calculations and data reviewed: ov Comments:

hnvironmnental lesting Solutions. Inc. Page 3 of 6 tnvironmentai Testing Solutions. Inc. Page 3 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: I/-g-b4.

Survival and Growth Data Day 43.9% 72% 100%

M

_ _ _ N 0 P Q R S T U V W x t/) J _ _ - /6 /6 16

/6 /6 /6 1a 10 /0 /0 10 /0 /0 /0 10 10 to /D 10 2 /0 to IC) 10 /0 1O 0 I0 10O O lO 10 3 /O-O /0

_ _ ///0 /0_ _ l/b 4

_ _IC)__ _j

/0 /0

/

'lb 1o o 10 1 o o /Ito0/)

_/)

10

/0

/c I

/0 to I Ib Ib /G/t Io lolC C) ID /O> Id /0 I0 7 10 _ to 10

/6 { / O/

A - Pan weight(mg) _ - S -

Tray color code:: 3 'A -0 Analyst: fl E&UL 1 ~h B - Pan + Larvae weight (mge) eMX ; to x X p $\y\v w w 2 Larvac welght (mg) - A - B _ _

. v\ 9' W \JI 4 w ' 9 Weight per Initial number _ _

of iarvae (mg) O O? D t@ p S aO O C/Initial C nunmber of larvae \9? V Average Percent weight per reductionO.So5R5 3e initial from control number of (%)

larvae (g Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

Environmental Testing Solutions. Inc. Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:

Survival and Growth Data Day 100% Intake Y Z AA TBB 1 /0 IC) /D /LD

/ 10 /0 /0 2 /o _ / /0 3 / 10 /0 /0

.4 _ /0 / I 5 __ 10 1 0 10 6 1o to 1o 0 7' _ _ IC) _

At - Pan weight (mg ,,_

Tray color code:: b a Analyst: OCA CKa B - Pan + Larvae weight (mg)

Analyst: ...... L........- \..C Larvae weight (mg) - A - B Weight per Initial number

. orlarvae(mg) AV C I Initlal number oflarvae O \0 O- O' Average Percent weight per reduction Initial from control 0 3:5 -11a number or (%)

lurvae (ma)

Comment codes: c clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

TVA I Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA821-R-02-013, Method 1000.0)

Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyzes TVAI Seqo iNuclear inast, Owntft 101

Nemueted Teat date: Nmvnberg015,2004 Proeecd A smber 1557 Revetwed br.

hL=I A 30 30 13.01 20.72 5.71 0.571 0.571 catrl B 1o0 ; 10 14.64 20.20 5.56 0.556 0.573 5.6 0.536 100.0 0e3 5.S nt apptleable C t0 10 15.04 21.2.2 6.18 0.610 0.610 D 10 10 14.38 19.04 5.46 03546 0.546 i 30 30 14.20 20.s9 6.61 0.661 0.661 1091 .. L.. 30 30 14.96 21.63 6.69 0.669 0.656 6.2 0 669 I00.0 0.555 ILI -14s G 10 10 14.32 21.26 6.94 0.694 0.694

_H 0 t0 15.13 21.17 5.99 0.599 03599 I 30 10 14.91 21.49 6.58 0.658 0.658 22% _ to 10 0 s4.7 90 5.9920 0.520 0.570 11.1 0520 10w. .0.e K 10 30 14.80 20.13 5.33 0.533 0.5333 L 10 to 14.44 20.44 6.00 0.600 0.600 M 10 10 1-14.37 21.33 6.96 O696 0.696 4.9% N 10 10 14.36 20.91 6.55 0.633 0.650 4.5 0 655 8o0.0o.55 4.5 .14.9

_ to 10 14.8 21.11 6.23 0.623 0.6Z3 Qo 10 1 14.59 6.26 0.6260.626 71% R 10 30 14.90 21.25 6.35 0.635 0.590 1.4 0.635 1).o 0.52" 8.4 .4.5 s 10 10 15.03 21.10 6.07 0.607 0.607 T 10 t0 15.32 2057 5.25 0.525 0.525 U 30 30 14.05 20.05 6.00 0.600 . 0.600 100% V 10 10 14.02 20.40 530 0.550 0595 5.7 0100.0 0.S5 . 5.7 -3.1 W 0 t0 15.10 20.92 5.82 0.582 0.52 X 10 10 14.95 21.33 6.30 0.630 0.630 Y 10 30 14.98 21.27 6.29 0.629 0.629 100% Itake Z 30 1o 15.06 21.50 6.44 o.644 0.635 3.7 0.644 3o00 0.J35 3.7 .80.

BB 10 10 14.87 20.93 6.06 0.606 0.606 owfnl 1l: MSD- MiKknmS tilicarDiffmence 0.0739 PMSD PeretMinumnSiifimat Difference IMSD: 12.0 PMSDIanm*c, 0(3.8 tmim the PMSD ia the mfarmmpcmt diffUasc bew the wotrl and trmbinmt that cmnbe declared P tcWlly 8lcifiant in awhole effluent toxicity tet.

Choamg, a uipicmst dfferncee o r for Earomuntal Testing Solutiome Inc. chronic toxicity tut wien a toxcant reduces Proophdea Vvnh by 15.6% fronthe oontol (detemred thouai reference taxicknt testing.

Dwusetts MaD voah: 0.0306 LoPWpMSDounddetemedbyUSEPAA(1Othpentile) -9.4%

FMSD: 6.7 U ePMSDbound deteinedbyUSEPA(90th poeetit) - 35%

The towwrdpperbonde mwea ecladtd by th USEPAwuisg205 testscmudcted ftni l9 ltostorieeformepda gOwth imchroncref e toxicant tats.

USEPA 2000 Undtndg andAccotIng ro Method Vwabin Whole Effluent Toxicity ApplktioUnd te N nl Pollunt Diohv Elrmation IuSn BPA-0331..O000. US Envdm Apc.,Ptoection Cincadt

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Larval Fhkl Graw sad Smrvival Tol7 Day GrCw Stat ae: ID912004 TetlD PpPRCR SunpklDl. TVA/ SQN OI INbwnated EmdD"c I/1620C4 Lab D: WID-awvk Te . So dg Sape 2vpc D MRM c-p MFkogRepot Sape Dat: Pmtc FWCHR-EPA421-AR24013 Tat Sdeck W.Fhephaks pPomehs inmla:

Cea-% 1 2 3 4 D-Qontml 0.5710 0.5560 0.6110 0.5460 10.91 0.6610 0.6690 0.6940 Q5990 22 0.6590 0.5200 05330 0.6000 43.9 0.6960 0.6550 W6510 Q6230 72 QL6260 0.6350 0.6070 0.5250 300 .6060 0.5580 0.582D 0.6310 Ttsladm Uln~mpsta I-Tailkd leatek Cle-% Mesa N-Mma 9Me MI Mlx_ CV% N t.Stt 01&Ie M9S) MS a N.U D-Cmtfol 0.5721 lim 05721 05460 O.6110 5.564 4 0.6161 1.LOOM 10on 0.6558 1.1449 0.6556 05990 0.6940 6.155 4 -2706 2.410 0.0739 0.6161 I.OXO 22 0o7 1.0067 0.57 0.5200 0.6510 11.071 4 .0.163 2410 0.0739 0.6161 1.0L00 439 0.6530 1.1418 0.6580 0.6230 0.6960 4.341 4 *2.7B0 2.410 0.0739 0.6161 1.0000 72 0S9U3 1.0445 0.5983 0.3250 0.6350 8.393 4 .0.131 2.410 0.0739 0.5923 0.711 100 0.5945 1.0310 0 5945 0 t550 0.63S0 5.672 4 .0.709 2410 0.0739 0.5945 0.9650 AzzI1ar7Tau Suti ldea cvkkl Skew Ear Sbapvo.W&I Teat he nxmldiatrfmtem (p > 0.01) 0.91021954 0.QI4 Q0.07339446 0.45529025 Bathh TeatbdkaeqWultlnc (p-0.71) 2.494269133 15.0627224 Hypp sl6Tmtm(I4WL*.OS) NOm LOIC 75V _ TU Us mSDp MSF MSI FS& di DanDe~ Tat 100 >100 1 0.073908152 0.129042062 0.0057612 OOOI.0t 0.035764091 5.11 l0vtmmb vs D-Orotl Lbear lalarpok"a CM Rnaseples)

Palt % SD 95% C1arp) Skew Ios >100

}C10 >100 1013 >100 1.0 MO >100 0.8 IC25 >100 a0 IC40 XOD C.

ICSO >100 0.6 05 jOA e 02 0.1 0.0, **.

0.1 4.1., ,-' ................

.0.2

.0..

0 20 40 60 80 100 120 D4s %

0.0 0.7 ,

14.3, 0.06 E J .ef sIgnlnlcce 0I.4 0.1 jqm_1-MU0da

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Larval Fbb Grwwth and Svr~vm1uTwW- DayGrowth SWlDale: 1119/2tO4 TastID. PpF SunieID, TVAISQN 101 Intaka-N i-tua1d End 1,: 1/J162004 aIbMD MR23-Twk. Tdf Sol Sanmph ra DMR-Dlech- ar m@fagRap-Sampl Date: ?rotot FWaMRPA421-R02-013 Test SpeIs: PPk-phaw PrgnU Cae-A% 1 2 3 4 D-Cofrol 0.5710 0.5560 0.61$0 0.460 100 0.6290 0.6440 0.620 0.6060 T7bfem:m U.5iufwWd I-TOW IMsie Cet.% Mm N-Mesa h^am min Max CV% N WSW 01dk) MSD M a N-Mma D-Contm1 0.5721 1.0000 0.5728 0.5460 0.61t0 5.564 4 0.6040 1.0000 100 0.6353 1.1091 0.633 0600 0.6620 3.733 4 .3.147 1.943 0.0316 0.600 10 AuxllryThb Sbt*& Oldel skew Kurt ShmpkoWilt Tedt ksuldatenmmd d 0bm (p> 0.01) 0 194020614 0.749 0.616194271 .037521312 P-Tot kSates equal wlena (p - 0.64) 1.106214301 47.46722794 MYp.G"bTat(Ql ." .) v MSp SS 905K MbI F-P & if HoameduzctTest dtunoantdiffi aneam 0.03t593456 0.0673U726 0.0078125 0O.0073U917 0019893471 1,6 Tmamenh , D-Coa*rol Lhw 1Istzpektim (200 Ramaphs) peot 5C SD S% MMr) Skew C100 >100 ICIO >600D 1C15 >100 1.0 1C20 b-100 e IC25 >100 0.6 IC40 >100 ICX50 100 0.7 0.6 0.4 0.3 02 0.1 e0.1 ....... ........ ..

-0.2 0 20 40 60 e0 100 120 Do" %

0.7 0.6

.140l 0.06 lee

............................................................. grcance OA 03 0.3 0.2 0.1 0

,wl_)J-0MO4dafa

Environmental Testine Solutions. Inc. Page I of 7 Environmental Testing Solutions. Inc. Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphniadubia Client: TVA Facility: Sepuovah Nuclear Plant- Non-treated NPDES #: TN 0026450 Project #: 155+

Dilution prepar tion info mation: Comments:

Dilution prep (Ne) 10.98 22 43.9 72 100 Emuent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organisminformation: Test information:

Organism age: . -N - tou tS COLb Randomizing template: Bwe Date and times organisms 11-0-Y COll -M IC)qb Incubator number and were born between: shelf location:

Organism source: I t-oz-oq A- F YCI batch:

Transfer bowl information: pH = E. ov Temperature = 2.5 A Selenastrum batch: ID.2&-O Daily renewal information:

Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time Jn 115 0 lI1 q.c4 j-.4 0 ofiiii3 ac 1-. .4 1 IJ-io0o4 i3O1 1b67-oq 4I IOt.c0 10o2- A 2 f11-.i-Dv izs?. Ikt-ok Hip+/-II . .OF 3 II.-IZD4 ILtC li-O-l Oc 1 lIaII I E o70ii 4 I-304 15e 3:5 7- II-OR-04 0 oCA 1\2. 0% I-tZ CK S 11-liqttq 15Z(O 1%-12-C60 OA 1812 17- 0% °O 6 1 . 1515-IZ-qI 12...oI .0-i-7 ,IJ.J.DI1O253 __! A L Controlinformation: Acceptance criteria Summary of stendpoints:

% of Male Adults: 0% I I 520% 7-day LC5O >100%

%Adults having 3' Broods: 1 7. 10o7 2 80% NOEC 1tC0

% Mortality: 07' 0Z r 20%0 LOEC >ICC)%

Mean Offspring/Female: 2JD 29.3 2 15.0 offspring/fermale ChV > rIo

% CV: (.O 6______ 1,< C 40.0*% I1C25 > 1007O

Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: iH-Oq .o CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced Q CO Q ; C) 0O Adult mortality t 1 U [ U 1_- L- L-2 Young produced t) 0_0 _

Adult mortality L.. L - . - - = =

3 Youngproduced Q Adult mortality %-- L-l>>>

4 Young produced 4 Lk LA4 S k=

- 5 __ _ -

Adult mortalty l - n L- -

5 Young produced o 0i _ 9 0 1I Ij Iic IC)

Adult mortality I L l U - - Il- Il t l 6 b 6 Young produced i Adult mortality L L_ 1l-1 Ul L U J 7 -I I -11 16 Total young produced l 2 2r1 l3 Zi T29 2zc 31 31 l31 FinalAdultMortlity Xfor 3 Broods .I l..l l L- TZTI L.. C. U

>C-I >Z-l Ul C

5%

l Concentration:

%Mortality: l 07t, Mean Offspring/Female: -24.1 coxc: 10.98% Survival andReproduction Data

- - Replicate number -

Day __ _ _ _ _ _ _ _ 1 2 3 4 5 1 6 7 1 9 10 I Young produced l0l( )lC Adult mortality U. L L 1 L i . L? U. LnlC 2 Young produced l 0-C. IO. - I° - I C) r a -

Adult mortality ( l l c-*l 3- Youngproduced a -: -I-lt o)

C) llAdult mortality

L fl I 4 Youngmortality

.Adult produced 1l j . l l f S .it = IU S Young produced l9 - 1 Z l Adult mortality L l l U l l L 6 Young produced l 1 l l )l I I 0 Adult Mortality L l l U. l_ LI L I U

I .

7 Young produced l9O lF 1 I 15 l .Il A I Totalyoungproduced i3%4 l l 3Z a31I 1 29 31 Final Adult Mortality l T7 T 1 -

Concentration:

% Mortality: M j Mean OffspringFemale: 31.0 A l

% Reduction from Control: - I.VZ3 l

Environmental Testin2 Solutions. Inc. Page 3 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: - I-0t CONC: 22% Survival and ReproductionData Replicate number Day Adl oa3 t 2 4 RIcS l 6 lb 7 1 8 1 9 1 0 I Young produced Q _ c O o c l Adult mortality 1 LL 2 Young produced _ l Adult mortality1 . .. U U W jU.1 . C 3 Young produced l .C CS 0 lL C.) lI c 0 Adult mortality I L L? 1 L L..

4 Young produced 5 .. 5- S - S V . S iS S Adult mortality 1 U U . I_ \ . U 5 Young produced I2- lL I 1

.Adul-morality l - L

-l - - \ -

6 Young produced O0 O 00 0 Adult mortality L- _- - L.l- L.1 7 lYoung produced lL jI l l , 1L ,5 l ,1- I Total young produced l 3-1 32 3 l 3 lI 33 l w Z9 33 29 Final Adult Mortality Il1l L = l_T Lt l l Concentration:

l %Mortality: 1 07. _

]

I IMean Offspring/Female: I 3 .9 l I% Reduction from Control: I - i."P?.

CONC: 43.9% Survival andReproduction Data I - Replicate number _

l Day l 2 l 3 l 4 l 5 6 l 7 l 8 l 9 l 10 l l I lYoung produced l C \l< ){° lA I O lC l l ~~Adult mortallty l (1 L1 L I l l2 lYoung producedl ) ) C) O O O 01 1 l l ~~Adult mortality l _ l l l _ l l l l l T 1 l3 lYoung produced l )lOl6 >lO C)O O C) l Adultmortality L- l- - L- I 2

4 Young produced ()

5 p p 0 0 2- QS- 0 0 l Adultmortality l 7 Young piroduced - - - - -

-(dlmrtlt/ IL U.U 0 UIL LILS IL Adult mortality - l L- U U L - -

4oa young produced SIN3 aq ..5.. Lc7- T..54 3:S 3i l Fnlla Adult Adulorllt Mortality mraiy l U- U. l LL l L J LL- L- l L lL -I Concentration:

MMortality: f I MeanOffspring/Femalr: l

%Reduction from Control: -'.7%

Environmental Testinz Solutions. Inc. Page 4 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: ( /- np CONC: 72% Survival andReproduction Data

-Replicate number Day l 2 3 4 S l 6 l 7 8 9 l 10 l Young produced l- - _ c __.

lAdult mortalitylI_ L 1 l 1 3 Young produced l O fl ( 0CQ Q ) O Adult mortality l l_ U U C j U. l 4 _ Young produced L4 5 S _ LA_IS__

, Adultmortality -- t - l . L-s ungproduc I _ I O3 Adult mortality l -

6 Youngproducedi O l l OL l - C) 0 Il l Adult mortality j l L j U.1 I I L-- 1_ l.- l 7 Youngproduced j20 I j-j 1 IQ1!LILLi I 1 j 1j 2j Ill Tota young produced i333 S 31 T3 3 3 Fnl Adult Mortality it y I I l I I Concentration: l

% Mortality: O l Mean Offspring/Female: l 3L4.1 l

% Reduction from Control: - I1.ZI CONC: 100% Survival and ReproductionData Replicate number I Day 1 2 3 4 5 6 7 8 9 10 I Young produced 1 C) - . °0l 10 1 2 Young produced - I - - -

Adult mortality L( LL Il -- -

3 Young produced l C5 lL (O Adult mortality _ U 1. U.L L

(_ ( . UL LI l4 I Young produce l 4 ;l (10 L!> 9lS-t .* Sl Adult mortality l L . L- L LlL( l L l l Adult mortality Young produced l C C-) O I O O l I Z /= L10 U/ C I Adultnmortality UL L. U Lt 7 Young produced lO 1- 101 1Gt... l l C O 1..H 1q I Totlyoungproducd ie 30 3f4 35 31 l 3 l 3 l 0 5 0 1 3 l FinalAdult Mortality L l- l- L- l I \ l ll c tL Concentration:

%Mortality: r (0 Mean Offspring/Female: I

% Reduction from Control: - I1

Environmental Testing Solutions. Inc. Page 5 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: //-0q-Qj CONTROL Survival andReproductionData R-p-icate number I Young produced - - -r- o - -,o Adult mortality L- U L- l_ _ L - L-2 Young produced

!Adult mortality C)_ o0 o _ _ 0

! Itl.-

3 Young produced Adult mortality '-

0t g L- L, -

7A l L UI cCI I 4 lYoung produced LA 5Li 3 LI i l

_I Autmortality IL_= ~

L I - -

cOt ql)

-eLL Young produced 1 O O 0

.Adult mortality -L L U k _U U U L _

6 Youngproduced Cj I I 00 I l Itl I C Adult mortlity U 7 Young produced ' I ILI 1 I T 13 1{ 1T Total young produced 'Z9 3L l 21 29 28 21 r-9 2- , 3 Z l 30 Flnsl Adult Mortality - l- l l TL L-L t Concentration:

%Mortality: 070 I

Mean Offspring/Female: l2I9

%Reduction from Control: -

CONC: 100% Intake Survival and Reproduction Data l l___ Replicate number Day l I1 2 3 4 5 6 7 8 9 10 I lYounig produced l C)1°l( ^ l°l°l(

Adult mortality U U U - U U l2 rYoung producedl l6l O C l° l l0 Adult mortality llll - -

3 Youngproduced

__ Q 0L- 1 L1r. 0s 0i 1 Q Adult mortality 1 U U l 1.U L l\ L lL lL 4 Young produced l L l 0 l q l 0 1 c l 5 lI l s l ~Adult mortalityll Youngproduced L r L- F l L I l tLL. LU I U l l l Adult mortality 6 Young produced l IU l - Tl I 1T l tJ- l l T l = l t -

0 Adult mortality CU U U UL 14 J

7 Youngproduced 1I 1 1Li 13 13 lI IS 13 1 Totalyoungproduced IzI 29 2R 25 21 1I 28 28 3l FinalAdultMortality L. I L l L l 1 U l L IT

I U Concentration:

% Mortality: 0 I Mean Offspring/Female: 71.0

%Reduction from Control: '4. 7

TVA I Sequoyah Nuclear Plant, Outfiall.101 Non-treated

'Verification of Ceriodaphmnia Reproduction Totals 72%-

Day!l- Repllcate number I- oa S 1 6 7 18 I9 I1I0 Day Rep'Icate number .-. ~.... Total Day 1 3 14 1 2 3 4 S 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0- 0 0 2 0 00 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 -0 0 0 0 0 4 4 4 5 4 5 6 5 5 4 5 47 4 S 6 4- 5 5 S 6 4 5 50 5 0 0 4 0 0 9 0 II 10 10 44 5 13 1-2 10 11 9 11 11 10 10 13 110 6 9 8 6 9 10 0 10 0 0 0 52 6 0 0 0 0 0 0 0 0 0 0 0 7 15 IS 18 14 14 15 14 15 17 16 153 7 20 16 21 18 19 19 211 16 20 17 187 Total 2111 27 33 27 29 30 129 1.31 3=113 1 296 Ttl 38 33 371 33 331 351 37 _32 34 351 347 10.98% __ _ _ _ _ _ _ _ _ _ _ _

Re lItete nuiber ~ 0 Total Day ~RepDicate numberTO I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 -0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 o - 0 0o 0 o 6 0 4 5 4 5 6 S 5 4 4 S 5 48 4 5 5 6 6 4 5 3 6 5 5 32

-5 0 0 9 12 1210 9 9100 71 5 9 0 0 0 0 12 10 10 10 12 63 11 9 0 0 0 0 0 10 0 9 29 6 10 11 -11 9 12 0 0 0 0 0 43 7 18 18 19 16 15 16 13114 14 17 162 7 116 18 18 16 20 16 15 19 16 19 173 Total 34 31 33 4 32 1 281 27 29 31 1 310 Total_ 301 34 35 31 36 33 30 35 311 361 331 22% Control-2 - - -- -

Day I Re Ilcate ntmberTO Day ep~tte nmberTotal 5 6 7 8 TotIal I 0 0 0 0 0 0 0 0 0 _ 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0-o 0 0 0 0. -0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0F 0 0 0 0 0 0 0 4 6 S 5 4 5 5 4 3 5 5 49 4 4 S 3 4 3 4 4 4 4 4 39 5 12 0 11 10 0 13 9 9 11 10 85 5 0 0 0 0 0 0 0 0. 0 9 1 9 6 0 9 0 011 0 0 0 0 0 20 69 10 10 9 9 I11 111 9 10 0 1 88 7 16 18 171 191718 141 15 17 14 165 7 6 19 14 16 16 14 14 [13 18- 117 157 Total 34 33333336 27TY29 3=3 2 31 Tota 29 34 27 2 2-8 29 291 26 321 301 293 43.9% __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 100% Intake __ _ _ _ _ _ _ _ __ _ _ _ _ _

D Replicate nunter Total Day -j- icate p-y numbTera 1 0 0 0 __ 0 0 0 0 0 0 1I 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 00 0 0 0 2 0 0 0 0 0 0 0 0- 0 -0 0 3 0 0 0 0 0 0 00 0 0 0 3__ 0 0 0 0 0 0 0 0 0 0 0 4 5 44 56 5 55 S S 49 5 I1I 1I 11 10 0 9 11 10 0 1 11 84 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 I0 0 0 10 0 20 6 0 12 10 10 9 9 10 10 10 10 100 16 16 19 19 16 120 19 16 15 16 172 7 4 13 16 14 12 13 13 15 13 16 139 Toal 3 31 34 34- _32 134- 135_ 131 30 132 1 3252 Toa 8 29 29 28 25 27 27 28 28 31 280

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA I Sequoyah Nuelear Plant, Outfall 101 - Non-treated Test dates: November 9-16,2004 Project number: 1557 Reveiwed by: _

Concentration Replicate number l Survival Average reproduction Coeffdent of rerut reducloo from

(%/) 1 2 3 4 5 6 7 9 l 10 (/) (offspring/femaie) nrlt~lon pooled coirot (S)

Control-I 2S 27 33 27 29 30 29 31 31 31 100 29.6 6.6 Not applicable 10.98% 34 31 33 34 32 31 28 27 29 31 100 31.0 7.S 4.7 22% 34 32 33 33 33 36 27 29 33 29 100 31.9 8.5 .7.8 43.9% 32 31 34 34 32 34 35 31 30 32 100 32.S 5.1 -9.8 72% 38 33 37 33 33 35 37 32 34 35 100 34.7 5.9 -17.2 100% 30 34 35 31 36 33 30 35 31 36 100 33.1 7.3 -11.8 Control - 2 29 34 27 29 28 29 29 26 32 30 100 29.3 7.9 Not applicable 100% Intake 28 29 29 28 25 27 27 28 28 31 100 28.0 5.6 4A Outfall 101: MSD - Minimum Significant Difference Dunnett's MSD value: 2.282 PMSD - Percent Minimun Significant Difference PMSD: 7.7 PMSD is ameasure of test precision. The PMSD is the inimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant differeace occurs for Environmental Testing Solutions. Inc. chronic toxicity tests when Intake: a toxicant reduces Cerfodaphnfa reproduction by 10.0¶', from the controL Dunnett's MSD value: 1.530 Lower PMSD bound determined by USEPA (10'percentile) - I 1%.

PMSD: 5.2 Upper PMSD bound determined by USEPA (90' percentile) - 37%.

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphniareproduction in chronic reference toxicant tests.

USEPA. 2000. UnderstandingandAccountingforMethodVariabilityinWholeEfiluentToxicityApplicationsUndertheNationalPoDutantDischargeEliminationProgrm. EPA-833-R00-003. USEnvironmentalProtection Agency, Cincinnati, OH.

TVA / Sequoyah Nuclear Plant, OutfaRl 101 Non-treated Statistical Analyses Caridsphals Sarvivl ond Reprodsedo Tat.",red acs StartDle I3U9/2004 Tetd D:. CFRCR sampleU)IVAISQN 101 .Non-tatod EndDatc lt612004 Lab M. En avir.Tetig SoL Sample ¶7pc DI)R1DrscharstorixgRspoit Sample Date': Protocol FWCI-EPA-121-R02-013 Test Species: CD-Ccridaphnk dubb Comsmmb4:

Case% 1 2 3 4 S 6 7 a 9 la D-C=skl 2.000 27.000 33.000 27.000 29.00D 30.000 29.000 31.000 31.000 31.000 10.9S 34.000 31.000 33.000 34.000 32000 31.000 25.000 27.000 29.000 31.000 22 34.000 32.000 33.000 33.000 33.000 36000 27.000 29.000 33.000 29.000 43.9 32.000 31.000 34.000 34.000 32Coo 34.00 35.000 31.000 30.000 32.000 72 35.000 33.000 37.000 33.000 33.000 35.000 37.000 32.000 34.000 35.000 100 30.000 34.000 35.000 31.000 36.000 33.000 30.000 35.000 31.000 36.000 Trwror Ustramstwoned 1-Tiled lboak CSC.% Mesa N-Mess Memo Mi . Max CV% N t3sbt aiIkkl MSD Mesa N-M s D -Cotrkol 29.600 1.00 29.600 27.000 33.000 6.605 10 32133 1.0000 10.91 31.000 1.0473 31.000 27.000 34.000 7.754 10 -1.403 2.237 2.2t2 32133 1.0000 22 31.900 1.0177 31500 27.000 36.000 8.547 10 -2305 2t7 2.212 32.33 1.000 439 32500 1.0900 32.50 30.000 35.000 5.077 10 2.906 2.2t7 2.2t2 32.133 1.0000 72 34.700 1.1723 34.700 32.000 31.000 5.929 10 .5.111 2.2t7 2.212 32133 1.0000 100 33.100 1.1182 33.100 30.000 36.000 7.325 10 .3.501 27 2.212 32133 L100 Am ifaryTfub Stbdtu itlol Skew ICsrt Ylcmogsrov)Testindres trnml drtrutno 0(p>

01) 0.760670602 1.035 -0.17547715 -0.15392101 Badletri Test kticstes equal variances (p- 0.75) 2.704277992 15.0t627224 Hy 4the Tet (1,-b4 0.0S) NOEC LOEC ChV TU MSDs MSDp MSB MSE F-Prb dr DunettsTest 3oo >300 1 22t1579492 0.0770503U 30.1266667 4.9777J7J7 13E.04 5,54 Thnettmnts VsD-Cotcol Lsesr slabrp tloo (200 Pisoplks) polot % SD 95% 0. Skew IC05 >100 ICIO >100 ICIS >100 1.0 lC20 >100 OS IC25 >100 0.8 IC40 >100 0.7 ICSo >100 0.6

° O.3 0.2 0.1 0.0

-0.12.--

0 20 40 60 s0 100 120 Dose %

30 I all. 0.05leve of sxlgtfcancs 320 is 10 a*

wqJI.09-0440dal

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses Ckrdaphah Srvtvd tad Reprdecactl Tat-eprodactlea Stut Date, IW/9r20C4 Test ID: CdFPRCR Sample ID. VA/ SQN 101Intake- Non-treated End Dotc V116/2004 lab ID: BMEaTk.TeathntSOL Sample pe: )MRD g RMonlor e1pot Sample Date: Pwotoco FWCHR-EPA-t21-R-024013 Test Species CD.Cedaphrni dub is C.-%

_ 1 2 3 4 5

7 8 9 1i D-Corol 29.000 34.000 27.000 29100 28.000 29.000 29.000 26.000 32000 30.000 100 2S.000 29.000 29.0Do 28.000 Z.00 27.000 27.000 2S.000 2S.X0 31.000 TroastoruL Uatlt irnfead I-Talled Isotoek COac.% Me NMesa Mbm mi Mal CV% N 1-Stat Otlde Me" DSIM NMean D-Control 29.300 1.0000 29.300. 26.000 34.000 7.890 10 29300 1.0000 100 28.O00 0.9556 2.000 25.0DC 31.000 5.5W4 10 3.473 1.734 1.530 28.000 0.9556 AotitlaryTest Seatltic Otdcal Skew Kart Sbapko-Wiks Tet kidmtes ==dm detd'uton (p> 0.01) 0.937893212 0.S61 0.606024152 1.007873126 P.Test bdicates equ vadarm (p - 0.26) 2.116363697 6.541089535 HypMetle TOat 1 0.05) MSDv MSDp MSs MSE F.Prob dr HomosedastietTestkIdicatesro sififlardiff endea 1.530392338 0.05223182 8.45 3.894444444 0.15020228 1,11 Trstmcats vs D-Control Unearlateepdadon (280 R sFamplea)

Pont SSD 95% CL Skew IC05 >100 lC1O >100 ICIS >100 1.0 IC20 >300 0S IC25 >100 IC40 >100 0.8 ICSO >100 0.7 0.6

&0.5 0.4 02 0.1 0.0 0 20 40 6o 8O 100 120 Dose %

Dae-Raspesa 881t 14an. 0.05 WlA ofStgnllcartce

_25 I20 10 5

84 I

sqnJ.-09-04data

Environmental Testing Solutions. Inc. Page I of 6 Environmental Testin2 Solutions, Inc. Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: TVA Facility: Senuovah Nuclear Plant - UV-treated NPDES #: TN 0026450 Project #: /5?

Dilutionprepar information: 7 Comments:

Dilution prep (°/) 10.98 22 43.9 72 100 Emuent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:

Organism age: Zt.75 -m Z3, 2$ S oLb Randomizing template: L Date and times organisms 1O-OGt-4 o vS ThOO 530 Incubator number. 3C were born between:

Organism source: 1f- M PBS 8 ci-\ Artemia lot number. Ito5S Transfer bowl information: pH Temperature = °C Total drying time: ZA l5cA3o$

_ _ 8

_ _ _ _ Z~i* Date ITime in: 1o-cAOA 137w Average transfer volume: e Date /Time out: ll-noL4 t33,,

. . Oven temperature: l1'C, Dailyfeeding and renewalinformation:

Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time M? tt S 0 1/-o -fL. lt4Co 1310 1I-oS0o r 11 D'.o (.. l I to-bt' paso ISOc- Irzo it-cncA4 lit48.o t /J.oz 32 1 2i 01 S1 U I--t 01l 11o -. ,

2 I1.-3ict Cfb3 ~scO ~ 1Z4 1~Q1,L c 0-1 III011.0 I.GZ t /.

s 1j1j04J o0 10 %SoO IZ2A II-Ocki4 at 11I12.0o lm 1 3 11 Iqq Oils ISOzO j 1!- 11-1 204* O1112.01 l.02 ___

67I 15.tq O 1500 17zi 4 ii z.log C$4t112l j-,1.oi ..

7 IIOD ?l 4R' 'N~" E Controlinformation: Acceptance criteria Summary of t endpoints:

%Mortality: 2.75 i s20% 7-day LC50 > 1O070 Average weight per initial larvae: 6.B6Sb I . NOEC l 1io7 Average weight per surviving larvae: 2 ~ 0.25 mg/larvae LOEC ) (C67.

ChV > 1ItO'7.

IC25 I > Ido

Environmental T'estingy Solutions. Inc. Page 2 of 6 Environmental Testing Solutions. Inc.

Page 2 of 6 Species: Pimephalespromelas Date: fl-m-C)'I Client: TVA / Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day CONTROL 10.98% 22%

_____A B C D E F G H I J KI L

_ _D 0 / )6I /b 16/ /0 /0 /6 JO/0 1_

1 JO 1 JO 10 §O ' 10 t0o 1010 I0 '0 2 Ic I(

'0 I10 1o0 lO 10 10 /)0 I0 I0

.D I D//0 1 /0/ /0 /0 /0 /6 /0O

6 4 to 1olO l16 1 j /t) b( 6 16 ID

/ /0o / qSA IC /0 10 , I/ 1IC /66 '0 6 ID lO -10 __ /D /0 /' 10 IC

_ 7J (0_' I 0 P)0 /0 1 I0 1 l° A - Pan weight (mg Tray colorcode:: mIA M \ ° 2 -i A j@

B - Pan + Larvae weight (mg) - ,

Analyst: _ 2' I ,. 0 Larvae weight (mg) - A - B

_____ ~ @ M e he C, 9 b Weight per Initial number or larvae (mg)

C I Initial number or larvae

%9-7 'A

\9 ty IN 0.

IN bk DI e0I Average Percent weight per reduction initial from control O. (oBto O. t IS S%7

_1, O-bI b- - 1.5-number or (%)

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

I E~nvironmentai iestingy Solutions. Inc. rageiotox tnvlronrnen cal i esfln2 Solutions. Inc. rage i oX b Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: II-bq)L Survival and Growth Data Day 43.9% 72% 100%

M N 0 P Q R S T U V W X 0 A /0 /() IL) /0 /f -/0 /6 1/6 /6 1/6 O I)0 /IO ) /D JD / 0 /t/O ID 10 /

2_

IC)I0 1 1010 010/I 100 0O/0 10 3 IC) */O IO 10 _ - IO 10 10 0 /0 /) (0 4/ 16 16 fo 1, Io 16 /1 /6 16 16 /1 5 10 1 C) /0 /010 lO 10 10 /0 /0 6 __ IbX 10/00 / /O/IJ /O /0 /O /0 I0 10 -10 to l /O /9 lo Ilo /O lo lo A - Pan weight (mg)

Tray color code:: bLM Analyst: C C1 ¶gAfv C V \%;$ 0 \P w l¶:

B - Pan + Larvae weight (mg) q34%~,

Analyst: \' ,\g ,. dIC L°G Larvae weight (mg) - A - B

_ _ _ w _ _ _ _ _ _ .'s wt AN 1N Weight per Initial number -

-C/Initial number otlare O 5 ' O' O '\o0o Average Percent weigbt per reduction Initial from control O(oW number or (%

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. I Calculations and data reviewed:

Comments:

Zrnvirl-U111CIlALm le sttrn bolutions. Inc. rage '4of o jrixru"i'zeuia*z iesung OIutiOfls, IflC. rage 44 010 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: 11-oq-64 Survival and Growth Data Day 100% Intake

_Y Z AA BB 0 /6 1 t /0 /t /0 2

IC /0 IO 10 3 /O /' /O to 4 ()h to /(O

.__ IC) /() /0 /C 6 /O0,,

____6 7. l'O (0100 /

A - Pan weight (mg)

Tray color eode:: h .

Analyst: C. #\P-At(ALtM&L \13 X B - Pan + Larvae weight (mg)

Analyst: 2. L) , ,

Larvae weight (mg) - A - B

.. X' a. A t Weight per Initial number of larvae (mg) or 0l ^ e

-C I Initial number of larvae \'

Average Percent weight per reduction - q Initial from control ( 2-number of (%)

larvae (mg)

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed:

Comments:

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Chronic Whole Effluent Toxicity Test (EPA-821-R.02-013, Method 1000.0)

Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA /Sequoabp Nuclearsut.tOtfaDl 101oUV treated Tat dates Nuowder 9-16,2004 Project Iuu*,rn 1557 Reveedby: A C-badu ue _1wuene P W I i ma - Wut bw k A ftti R-f rer LP"ta w+e Werbi3iSu*" Me.. Wbg CI In. Wdut IbS MPwvftm H.- wdgbI Cofiduea rwm _ b

(.3 udgm two - A.3 a~Id,. u)S.vv

_ re,. _>

. t t A.~d(%

d.j

. ~ dh .(~

la__

M..

K IMdalm."ot (old)

,whUM"Of.

WWU._# >

e.a.t A I0 _ 0 14.72 20.35 6.13 0.613 0.613 Control B 10 10 14.9S 21.S4 6.86 0.676 0.652 5.4 0.6S6 97.5 0.636 8.7 Not ppocable C I20 t0 14.99 21.76 6.77 0.677 0.677

_ D 10 9 15.05 20.74 5.69 0.632 0.569 E 10 20 14.7 21.56 6.85 0.685 0.685 F 20 10 15.13 22130 6.17 0.617 0.645 6.3 0.617 100.0 0.645 6.3 -J.3 G 20 t0 14.66 20.63 5.91 0.597 0.597 H 10 10 14.64 21.43 6.79 0.679 0.679 10 10 15.04 21.08 6.04 0.604 0.604 22% J 10 10 14.9 21.75 6.79 0.679 O 0.679 200.0 0.646 4J .2.5 K t0 I20 14.33 21.32 6A9 0.6-49 0.649 L t0 10 14.59 21.11 6.52 0.652 0.652 __

M 10 t0 14.85 20.01 5.16 0.516 0.516 43.9% N 10 10 14.72 19.86 5.14 0.514 0591 14.9 0.514 200.0 0.591 14-9 7.1 o 20 I20 25.26 21.32 6.66 0.666 0.666 I0 10 24.70 1 2138 6.68 0.668 0.668 10 20 14.66 20.5 5.92 0.592 0.592 72% R 20 10 14.87 21.26 669 0.639 0.653 7.9 0.639 100 0.653 7.9 .2.6 S 20 20 14.61 21.27 6.66 0.666 0.666 T 10 10 14.91 22.06 7.15 0.715 0.715 U 20 20 14.56 21.71 7.15 0.715 0.715 lo00 V 10 20 14.75 20.36 6.22 0.61 0.660 13.6 0.611 200.0 Q660 13.6 -3.7 W 20 I20 26.05 21.65 5.60 0.560 0.560 0 0 'X 14.15 21.69 7.54 0.754 0.754 V 2010 14.49 20.86 6.37 0.637 0.637 500% l ntake 20 o 10 14.64 2258 6.94 0.694 0.632 6.2 0.64 100. 0.682 6.2 7.1 AA 20 10 14.97 222 67.34 0.734 0.734

_ __ _ _ 3a__ 20 20 14.39 21.00 6.61 0.6621_ _ _ _ _ _ _ _ _ _ _ _ _ 0662_ _ _ _ _

Qrdal 1012 MSD - Minimum SileficntDiffneace Danett's MSD value OL1087 PMSD- Percent Mim PMSD: 17.1 PMSD h ameasr ofest pecision. The PMSD is the mfinmm percet difernce between the contol nd atnt that cm be doclared statistically significant in awhole eftent toxicity t. Om average, a splifleant difference occur lbxEsvlontntal Testing Sotutiont6 Ine. cronic toxicity tests when a toxeiat reduces P rmtpokla growth by 15.6% ffm the control (determined through Inbakei reference toakat testing).

Duamett's MSD valu: 0.0676 Lower PMSD bond demned by USEPA (1Oth peratie) - 9.40 rMSD: 106 Upper PMSD bound determimed by USEPA (90th percentile) - 35 The bwer d pperbounds were cain ted by the USEPA sng 205 t conducted n29 bboeatmies forPfamphate growth in chtic refence toxicant test USEPA. 2000.Uderdig ad Aceountiag oeMedtodViablityin Whol EluentToxkityAppcations Underthe National Pollt Dcharge Em ao Pro FEPA-833-R4.0 US En nmestal PotectnAgency.Cinnnat.OIL

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Statistical Analyses Lami YFIeGrww and Svra Tm-7 Dy GrAwi StatDatc: 11 04 Tat MD PpRCR samp la. WA/ SQN 101- UV-buWd Edtr 116t4 LaID. B n*. Tufts Sol Spk 7Type: DM R4 h Moa3ftRep-t Snph DIAt: Piot o WCHR-PUA421-RP02-013 Tat Specim Pp- i~ pomcks Cbft.% I 2 3 4 D.0mml 0.6130 0.6S60 0.6770 0690 to0. 0aso Q6170 0.5970 06790 22 0.6040 0.6790 0.6490 0.6520 431 0.5160 05140 06660 0.6m60 72 0.920 0.6390 06660 0.7150 100 0.7150 0.6110 0.600 0.7540 Tnabwm: Un dnf~d 1-Talad Ian;nI C_.C% M." N-M. Mean -a Mal CV% N Atat OI MSD Mea NIMtd DConm4 0.6363 1.0000 0.6363 05690 0.6a60 - .703 4 0.6423 I.OXO 10.91 0.6445 1.0130 .6445 05970 0.6130 6848 4 .0.183 2.410 0.1087 06423 1.000 22 0.6460 1.0153 0.6460 0.6040 0.6790 4.t11 4 0216 2410 0.1087 0.6423 1.0000 43 0.5910 0.9229 0.5910 0.5140 0.6690 14.tS0 4 1.003 2410 0.1087 6347 0.912 72 0.6530 1.0263 0.6530 0.590 0.7150 7.t 4 .0371 2410 0.10 7 0.6347 .9t2 100 0.6600 1.0373 0.6600 05600 0.7540 13.626 4 .0527 +/-410 0107 0.6347 0.892 AsIffluS Tub staW& OlkaI Skew Knrt Sbpko-Win Tat kidate -- aldAWbico(p>0.01) 0W619902 Ot.4 40.765099 .121340363 DaTr Ted Tat s h "1 W yrknm (p Q-53) 4.153M67 15.0E627224 Hypm IoTwt(l14I*."S BIIC LOKC ObV TU MSD MSWp M65 Win FP-rn if Dc arsTat 100 >100 I 0.10O702625 017034t606 QOD242D42 0.004063875 0.7041E6499 5i1 Thakeln w DGmtrta Llmer Intrpsatkm (3M Regaqlu)

Paint -% SD 9% COUsp) S IC05 >100 ICIS >100 1.0 IC10 >100 IC25 >100 IC40 °° 0.3 ICSO >100 0.7 0.6 02 0.1 s.4.:

0.0 " .............

e0.1 0 20 40 60 sO 100 *120 Den%

0.8 0.7 0.6 0 .5-....................... ...... ...................... I41

-tal. . sWet 0.5of nignificarnc.

0 0.4 0.3 0.2 0.1 0E -

aqnJ!.9.Oda'a

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Statistical Analyses Larval MshGrwf andSorvI Tet.? Day Grwwt SlafDfta IU912004 TeatID, PpFRCR Sampik II) TVA13QN0Ot1 - UV-lted WW3 Rod Datc IlU16JX0t Lab M. M-tTeflktiSot SBmple 1Ypv D bdcp MonDti Roport Sample Date:Protooo WCHR-2PAU21-R-02-013 Test Specm P.nUp Cbnu-% 1 2 3 4 D-Cmitrl 0.6130 0.6E60 0.6770 0.690 100 0.6370 0.6940 0.7340 0.6610 Tnxsisels: Utawatn d I-TaIW boak 0mc-1 Me NMe"m M"am Mk Mal eV% N t1-Sht Caida MSD Ma N Mna D-Conbul 0.6363 1.000 0.6363 05690 0.6E60 J.703 4 0.65%9 1.0000 100 0.6915 1.0711 0.6815 0.6370 0.7340 6.175 4 -1301 1.943 0.0676 0.65%9 1.OXO AaniliesryT Statisde OIC a Skew Kae Shapmo-W& Test ipdutex nmal dwtim lx > 0.01) 09160E9B33 0.749 .0.15333942 -1.60140066 P-Test bdvates equalvmioe (p - 0.66) 1.731366515 47A6722794 ky? uTest (1.4m%,SAS) MSDl MSDp MSO MST F-Pr.b at Hweduti t Test hidano a*6can diffaeaw 0.067574427 0.106207352 0.004095125 0.O241%625 0.24015015 L6 Troafnen D nl Lbmrlmowpokfi (214 Roou"Ms)

Pert % SD "%C142p) skew IC05 >100 ICIO >100 ICIS >100 1.0 IC2D >100 0.9 IC25 >100 0.0 IC.40 >100 1C50 >100 0.7 0.3 I 0.OA 03 02 0.1 0.0 .~

-.01

.02 0 20 40 so so 100 120 Do" %

0.0 0.7 0.6 14a3, 0.06 lewI 0.5 .of aignfcac tOA0

,03 02 OAt 0t sw _l.09.04data

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalespromelas Daily Chemical Analyses Client. TVA/SequoyahNudeai -Plant, Non-treated Test dates: November 09 -16, 2004 Project nmnber: 1557 Reviewed by:

Concentration Parameter Dal DDl Dy2 Day3 Do74 Day5 Day6 Initial lnitial IFnal Fnl Initial I Final Initial Final Initial I Final Initial Final Initial Final 11 (SU) 8.06 7.96 8.02 7.91 7.98 7.92 8.03 7.73 8.12 7.72 8.05 7.82 8.11 7.96 DO (m-gL) 7.8 7.8 7.8 7.5 7.9 7.4 7.8 7.0 7.8 6.9 8.0 7.7 7.9 8.0 Conductivity (glmhos/cm) 299 295 296 306 301 293 293 Control kalnity (mg/L CaCO3) 63 61 62 61 Hardness ngl CaC,) 89 83 83

_Temperature (C) 24.8 24.8 24.7 24.6 24.8 24.3 24.9 243 24.7 24.6 24.9 24.5 25.3 24.9 pHl (SU) 8.06 7.93 8.04 7.88 8.07 7.89 8.15 7.65 8.13 7.68 7.99 781 8.101 7.94 10D98% DO (nt/L) 7.9 7.8 7.5 7.9 74 8.0 6.9 7.8 7.0 80 8.0 7.9

. ConductivIty (jnhosunhc) 285 283 287 285 20 28t 290 Temperature (C) 24.7 24.5 24.9 24.5 24.9 24.3 25.1 24.6 24.7 24.6 24.9 24.3 25.3 24.8 pH (SU) 8.06 7.92 8.04 7.86 8.07 7.89 8.13 7.66 8.13 7.68 8.02 780 8.11 7.94 22% DO (mg/L) 8.0 7.8 7.8 7.4 8.0 7.5 8.0 6.8 7.7 70 8.0 7.7 8.2 8.0 Conductivity (jimhoafen) 263 26 2718 272 Temperature (CC) 24.8 24.6 24.8 245 24.9 242 25.1 24.4 24.9 247 24.8 24.5 25.1 24.7

n (SU) 8.03 7.90 8.02 7.81 8.04 7.86 8.09 7.68 8.09 7.66 8.01 7.79 8.07 7.93 DO (mgAL) 7.9 7.8 7.9 73 8.0 7.3 8.0 6.8 7.7 7.0 8.0 7.6 8.2 8.0 ConduLtvity (Ounhos/crn) 237 228 225 238 237 229 236 Temperature (C) 24.6 24.6 24.9 24.3 25.2 24.2 24.9 24.4 24.91 24.5 24.8 5.1 224.4 24.7 p11 (SU) 8.001 7.84 7.981 7.781 7.981 7.871 8.031 7.661 8.041 7.64 7.971 7.781 8.041 7.91 DO (ingL) 7.91 7.6 7.9 7.2 8.0 7.31 8.0 6.91 7.71 7.11 8.ot 7.51 8l2 8.2 72% (umhoveml -- ...

-a4* ...... Atn

.1** ani^On wt:onnneunlt Ternmerature (C) p11 (SU) 7.97 7.81 7.94 7.79 7.92 7.86 7.97 7.62 7.99 7.63 7.931 DO (mg/L) 7.8 7.6 7.9 7' 8.1 7.4 7.8 7.0 7.7 7.1 7.9 Conductivity (prmhostem) 150 141 148 149 140 139 100% Alkalinity (mg/L CaCOW) 53 51 52 Hardness (mg/L CaCOt) 57 59 54l Total Residual Chlorine (mg/L)I (0.10 <0.10 <0.10

_Temperature CC) 24.4 24.5 25.3 24.4 25.4 24.4 25.3 24.6 24.5 24.5 nIl (SU) 7.981 7.80 7.95 7.81 7.93 7.86 8.03 7.62 8.03 7.62 7.96

._ .. i-DO (mrAL) 7.8 7.7 8.0 7.5 8.2 7.6 7.8 7.1 7.8 7.3 8.0 Conductivity (umhos/aem w F z l

-1.1i-15143_i 140 pal (sup

= - - w-Z w e-s-100%o Intake Allcallnlty (nmzIL CaCtI,)

dty *fL C CO,)

11ardness (mg/L COCO3 )

l Total Residual Chlorine (mr/L)

Tem erature Ct) 24.71 l

24.5l 24.91 24 25.21 24A4 25.,1 244 24.81 24.6 2- -

rjnmvironmental Testing Solutions.c Inc. Page 5 ot 6 Tnvirnnmentlii Testing Sniti tinn Tn c,.. Page 5 ot6 Species: Pimephalespromelas Date: /1-95/

Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistry.

Day Analyst Concentration Parameter CONTROL pH (S.U.) 8.07 DO (mg/L) 1.

Conductivity 6izmhos/cm) z92 Alkalinity Z O (mg CaCO 3/L) S3. _t o Hardness e9 (mg CaCO3/L)

Temperature(0 C) Z( . .I V L4.6 %'4.

pH (S.U.) 89.0ko 1.3.04 .8 5.o1 18 10.98% DO(mg/L) . 1.9 1.7 Z. 79 1 Conductivity (pmhos/cm) Z's Z8S

_____Temiperature(C Z'47 24.-1F T1 4.9 pH (S.U.) Z .. 1. S. o 1.

22% DO(mg/L)

Conductivity Temperature (°C) Zl . Zr4.o 24.A Z.$ -24Ll.9 pH (S.U.) OT tZ ,8 .o4 z.3l -~t 43.9% DO (mg/L)

Conductivity (pmhos/cm) 5 2. 5

_____Temperature CC) z'.(. .C t2.2. 242 pH (S.U.) 19-oo 1 -7. 1 1- n.4R9 -7. - -7.q 72% DO (mg/L) I i.o -n -

Conductivity (Mxmhos/cm) I 3 qI Temnierature (° I.- I i.M.S 2 _ _ __

VS . I _

._ _F-5-3 pH (S.U.)

. 5, .--. _

-IALI.

I *-7

=

.-iq, -7.IZ. . _

I_

100% DO (mg/L) 71. I -7. t . 9-%

Conductivity (Hmhos/cm) IS5O0 lt4 Alkalinity 53 (mg CaCOJL)

Hardness (mg CaCO/L) 51 59 TR chlorine .IC) <0 (mg/l.lO Temperature (°C) Z41

% I : .7 pH (S.U.) *.c" 100% Intake DO (mg/L)

Conductivity (jimhos/cm) 1_ I Alkalinity (mg CaCO3/L) is -

Hardness (mg CaCO/L) 5 TR chlorine 4D.1 _ <0,10 Temperature (°) 2 Y. S 24. 3 2S.72 2 Initial xa] Initial l i

Environmental Testin2 Solutions. Inc. Page 6 of 6 Environmental Testing Solutions. Inc. Page 6 of 6 Species: Pimephalespromelas Date: //4q 6y Client: TVA / Sequoyah Nuclear Plant - Non-treated UI II Day I I 3 I 4 I 5 I 6 9 -

Analyst I C I P. I DfR I Oro Q I 1IAI I 1A I I Concen- Parameter tration I CONTROL I pH (S.U.) 1Z6.OSo5 '-. Z-s _..

C1%

s.u - .- I .,

DO (milL) LS ; ,. :iq 1_.t Conductivity (Pmhos/cm)

Alkalinity (mg CaCO 3/L) 5C4 -SOL 2_q3_

25. -6 Hardness

4. GIZ-5 (mg CaCO3lL) I Af Temperature (°C) I J.4. < I 21i.

4 7 -- II I 7 -

pH (S.U.) 5,C, . I A' I.6S. I I r w 7q4 IIII 1 l II ",.IO i1-qa I

10.98% DO (mg/L) I *1-1 1.a Conductivity Z9-0 Z-.1g57-

(pmhoslcm)

Temperature (°C)

I 1 2-,. I -

,di0 .0Z80 25.. Co 1 1 I

.z1.6 p I, -

pH (S.U.) a. I 7.6' I( -IS II .6?t I 3.oZ I -I.so TI 22% DO (mglL)

Conductivity J 1Z

-Ij.7 .D =,o 1. 1 1 -P-"

I (ymhos/m) ZI Z71 -~ Z&3 Temnerature (°C) 2q~w ' Z.9 .

I - --

pH (S.U.) 1b oe.61 0`1 9.0 I I'

-.L .

43.9% DO (mgJL) 1o~b~7 7 l0 9.0 Z.Z 0 .0 Conductivity (pmhos/cm) IZ 36'; _ Z.q 5(0l~

Temperature ("C)

ZLi. 3 z I z.X4 Zq1 tc =

2,4. _ _ I51 0

?4.1I pH (S.U.) 1. -7.66 1 fd.oVf IT1G 7. 79 1 In'~S-v -i.q 72% DO (mglL) .o0 I 6.9 I -'.7 .0 zq.0 _

Conductivity (pmhoslm) I l44 IqZ 2.'.L IgqJ I

Temnerature (°C) ZS1. I z4.Io 2'I. 2S-.Z I Zq.1

'I pH (S.U.) -i.q:71 7- 6I Z 1 71.99 1 - 63 1 I.c3 IiXU1A.0 100% DO (mrlL) I 1 '7.19 Conductivity (pmhos/cm) rJ9 }o N

Alkalinity (mg CaCO)/L)

Hardness (mg CaCO/L) i5,4 \-

TR Chlorine (mglL) 7<o.lc LI.(a ,2q. S 12-1 I Temnerature (°C)-,

- - - .- - -- -II pH (S.U.) 1 -i.6t I B.t I 7.62- -7.q6 1 7.9)4 - z I 7.sI 100%

Intake DO (mg/L)

Conductivity 119 -1

-1. i -

-7.8 1S B.o I IA .WIt I (pmhos/cm) 3 Alkalinity (mg CaCOVlL)

Hardness (mg CaCO31L)

TR chlorine (mg/L) L Temnerature ("C)

Initial L

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphniadubia Daily Chemical Analyses Client: TVA I Sequoyah Nuclear Plant, Non-treated Test dates: November 09.16,2004 Project number: 1557 Reviewed by:

Concentration IParameter Dayl1 l DaS I Day 6 L h - ffi l - .-. # - .

Initial I Final I Initial _ FInal I l

_ l Final Initial l Final I 4. -

oll (SfLn 8.021 8.081 7.981 8.071 05 8.03 8.11 7.94

4-DO (mrAL1l 7.8 7.9 8.0 8.1 7.9 8.0 DO (*.-

Conductivity (umhmslcn) I.-

295 296 Control Alkalinity (nigL CaCO 3) 61 62 AllHiniq (m6tL C CO3)

Hardness (mg/L C*COb) 83 83 Temnmnwaure eM 24.91 24-9 24.9 7111 71 7 71,c1 Idi1n q1

2) 24.

rriz l

-. -, i. ---4 8.061 7.98 8.04 I -4 8.07 I -I 8.071 8.151 8.00 8.131 7.94 plt (SU)

DO(ML) - 7.91 7.87.7.87.7.81 _ 91 8t78i 78I 10.98% 283 J..8 ~ 2861 28 7 j 285_

Conductivity (UnmSAcm) 2

- ^ - - _

24.9 A _ -

25.1 o

24.9 24.91 24.81 25.3 25.3 24.8 25.01 24.71 25.01 25.31 24.7 248 Tenpwrature pl (SU) 8.06 7.98 8.04 8.07 8.07 8.07 8.13 8.01 8.13 7.961 8.02 8.06 8.11 795 DO(nmg/L) 8.0 7.8 7.9 7.8 8.0 7.9 8.0 7.7 7.7 7.71 8.0 8.2 8.2 81 22%

271 1 262 283 Conductivity (nnhos/cm)

Tenperature(C) 24.8 25.1 25.1 24.7 24.7 25.1 25.1 24.9 25.0 2 24.9 25S1 24.7 248 pl (SU) 8.03 7.98 8.02 8.06 8.04 8.06 8.09 8.09 7.9 8.01 8.06 8.07 794 DO (nmWL) 7.9 7.8 7.9 8. 8.0 7.8 7.7 7. 8.0 8.2 8.2

. Conductivity ( rnhostem) 228 2 237 238 2 236 Temperature eC) 24.8 25.0_ 25.0 24.9 24.9 25.1 25.3 24.8 24.9 24.9 24.8 247 pll(SU) 8.00 7.94 7.98 8.03 7.98 8.0 8.03 8.W 8.04 7.96 7.97 80 8.04 7.91 DO (nw/L) 7.9 7.8 7.9 7.9 8.0 7.9 8.0 7.9 7.7 7.8 8.0 8 8.2 82 72%

Conductivity (uiholo/m) 193 18 1 191 ll 194 192 182 191 CC) 24.6 25.0 24.9 24.8 24.9 25.2 25.3 25.1 24.9 24.8 252 253 248 24.8 Temperature PI,(SU) 7.97 7.91 7.94 8.01 7.92 8.02 7.97 7.98 7.99 7.92 7.93 8.03 8.01 788 DO(nmgL) 7.8 7.8 7.9 7.9 8.1 7.9 7.8 7.7 7.7. 7.9 8.2 8.2 82 Conductivity (moscm) 1 S_

100% Alkalinity (mgtL CaCO) 5 5' 52 Hardness (ng/L CuCO 3) 57 59 Total Residual Chlorine (mWL) <0.10 Temperature (C) 24.5 25.21 25.1 249 24.9 25.11 25.21 25.11 25.1 24.7 25.1 25.3 24.9 24.8 11(SU) 7.98 7.871 7.951 7.981 7.93 7.991 8.031 7.94 8.03 7.86 7.961 8.011 8.021 7.87 DOW(n/lL) 7.8 7.81 8.0 7.8 8.2 7.81 7.81 7.8 7.8 7.7 8.01 8.11 8.01 8.1 Conductivity (,umhos/cm) 145 135 146 100% Intake Alkalinity (nWjL CaCO3) 51 50 hardness (mg/L CaCO,) 59 54 59 Total Residual Chlorine ( L) <0.10 <0.10 <0.10 25.0 25.1 24.9 24.8 25.11 25.21 25.1 25.11 24.91 25.1 25.21 24.71 24.7 Temperature (C) 24.5

- Environmental Testing Solutions, Inc. Page 6 of 7 Page6of7 Environmental Testing Solutions, Inc.

Species: Ceriodaphniadubia Date: //46?@ J Client: Seguovah Nuclear Plant - Non-treated Daily Chemistry:

Day2 Analyst -CA Concentration Parameter CONTROL pH (S.U.) 8.c1t .8 R'I DO (mgz/L) l- .8lg1.9 1.19 Conductivity (Pzmhos/cm) 2S2 .

Alkalinity LO 6Z (mg CaCO/L) I3_

Hardness (mg CaCO,1 3 Temperature (°C) VA '4.9 2qFq . 2 .1 2..

pH (S.U.) C 1°b O. 8.i 9% O 24 10.98% DOL(mg/L9 . 7jc -9 Conductivity (pimhosfcm)___

Temperature(°C) 5 ZS

4.' _ . _ ZL4.1 25. 3 pH (S.U.) I.OLP .00t _ .O _ R ____8 __

22% DO (mgfL) _ S% ,o0 Conductivity (pmhos/cm) Zl ZIPi7 Temperature(C) 1. Z5.1 25. I 24.1 24.. 5.1 pH (S.U.)

1.cI 04 _____

43.9% DO(mg/L) 1.

Conductivity (jimhos/cm) 7 -

Temperature(°C) Z'4.E Z.O 25. O i.' Z . 25.1 pH (S.U.) . O 1.9* ,0 1.°S ° 72% DO(m 1.£ £.O 1? -1 Conductivity

.(Vmhos/cm) I 3I2lli J' Temperature (C) 24. zc. O .q. 2 pH (S.U.) -1.tl .91 -7AAx 0 .7 A1Z. E;.

100% DO(mg/L) 1.

Conductivity

.(Vlmhos/cm) I 48 ib!.lL Alkalinity 3 (mg CaCQ3/L) 5 Hardness 57 (mg CaCO/L)

TR chlorine <0.10 Tmprate ( O.)

Temperature (C) 2'q.S I2 nj 2jqj VI.') 2%.

4 -

pH (S.U.) I1 -8 I1.- -7.fW I Q9 I 3 . I 100% Intake rna VV f-mrr- N tlIIIV ) - -X .t5t I Ir .e% 1 -- s 00 7_- A - c Conductivity (pmhos/cm) iq5 J3S Alkalinity

.. I (mg CaCOAL) _ II 50 Hardness (mg CaCO,/L)

TR chlorine (mg/l)_ <0. (t

~'j Temperature (C) zq.s I Z. O Z5, zq . 2st II d .- _- .

Initial I Final I Initial Final Initial I Final I

Environmental Testing Solutions, Inc. Page 7 of 7 Species: Ceriodaphniadubia Client: Seauovah Nuclear Plant - Non-treated Date: I(-o9-o64 a* I I 02V a I 3 I 4. I 5 I 6 Concen- Parameter tration l CONTROL pH (S.U.)

DO (mg/L)

Conductivity (umhos/cm)\ r - __t _

Alkalinity (mg CaCOItL)

Hardness (mg CaCO,/L) x _ __,_,..............._

Temperature (C)

.n 17S )

wl

",.\a0v. I-I I n. 8 -_, 11 1.- I _ - - V-' D 10.9 8% DO (mg/L) %.0 -1.% 1A£ -1.7 u 2_ 8.o_

Conductivity I 2g2 1 " - -- ?lo Ilig Temperature (°C) 25.3 Zs. 24. Zs.C) _21

- - ¶ I = . 5 . - ,1 - Wr-inT (PR I 01 I K U) I.,

_ -- D __, I _'S.1.7-7 AL i _ -,I-----*----

750Z.

L.-- a -_ - N -,

DO (mg/L) 22%

Conductivity (gmhos/cm) I I 7 17.7 2171

-1.-I

_- -26z. I a Z15 TF-erg-a ri ° X.\ II __VR I 27 7 _1- Zq.M 2S. 1 I 7q.I 1 -*

pHz~^Wuc ,SU. _. 9... t.A. _, _.

43.9% 'DO(mg/L) .O *. 7gZ Conductivity 37.:T (Hmhos1cm) Z3' _ 2.37 Temperature (C) 2S.3SJ VI.9 Z9.9 2 lI.

pH (S.U.)

3I.oo 6* ,6 91 l%0 $ 1-7.

72% DO (mg/L) 2t) 7.9 7.

Conductivity (pmhos/cm) _IN.

Temperature (tC) 253 25- j 2'4- 21.R . 2S.3 2 .J.

pH (S.U.) I ALqI. 7-9 Z 1.9 .O3 _____

100% DO (mg/L) l 7 7.

Conductivity filmhos/cmi) I1 LAO AlkalinIty :z (mg CaCO31L)

Hardness (mg CaCO/L)

TR Chlorine (mg/L) _0 _

Temperature (°C) X _2sY 100% DO (mg/L)

Intake _ _ _ _ _ _ _ _ _ _

Conductivity Alkalinity (mg CaCO/L)

Hardness (mg CaCO 3IL)

TR chlorine m fL < .1 Tcmperature (t 25 2.7 V. I ZS. I 2 i4.¶ZS-i .2.. 2.-

Initial I N .Final

.--. aI Inltlal Fl nal I Initial .. FIinal I. ntial Final

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)

Species: Pimephalespromelas Daily Chemical Analyses Client: TVA / Sequoyah Nuclear Plant, UVITreated Test dates: November 09-16,2004 Project number: 1557 Reviewed by:

Concentration Parameter iay 0 Day 1 Day 2 Day 3 Da 4 Day S Day 6 Initial Cno Initial i Final Initial I Ftnai Inittal Final Iniial Finai initial Final Pl1 (SU) 8.11 7.94 8.061 7.87 8.04 7.89 8.152 7.66 8.15 7.71 8.05 7.75 8.041 7.95 DO(mwL) 7.91 7. 7.8 7.6 7.8 7.5 7.9S 6.7 7.8 7.2 8.0 7.6 8.2 8.1 rConductivity (pmhoskem) 290 __ _3_

Cotrll alinity (mg1L CsCO3)____ _

llardness (mg/L CaCO3)

Temper ture C) 24.9 24.8 24.9 24.25 24.87 24.9 24.69 25.0 25.3 24.7 PH (SU) 8.08 7.88 8.02 7.84 8.09 7.84 8.12 767 8.12 7.64 8.04 7.75 8.10 7.96 10.98% DO (nt/L) 7.9 7.8 7.9 7.6 7.8 7.3 8.07 7.7 7.1 8.0 7.5 8.2 8.2 2 Conductivity (pmhos/cm) 290 _m_ 283 280 269 295 emperabreC) 24.9 24.8 24.7 24.2 25.1 24.8 247 247 2459 24.6 25.0 25.9 25.3 24.7 p11 (SU) 8.07 786 7.82727.83 8 8098 761 8.12 76 8.041 7.72 8.10 7.94 22O_(mL) 7.9_7.8 7.8 8 7.97.9 79 73 7.9 6.9 7.7 7 7.91 7.5 8.2 8.2 Conductivity O(mhos/cm) 273 _27C0_ 262 _ _2 284 Temperture (C) 24.7 246 247 24.3 25.0 24 24.8 24.7 25.21 24.6 25.0 24.9 25.4 24.8 pH (SU) 8.07 8.05 . 80 7. 7948 802 7.69 805 765 7.971 8.06 7.92 43_9% _DO(_ _ __L) 7.9 7.8 729 7.8. 6.9 7.7 7.0 8.8 7. 8.2 8.2 7 Conductivity (pmhos/cm) 24235 _ 236 _ - 2- =3 _

Te rture C) 24.8 24.71 24.4 25.1 253 24.8 25.2 24.9 24.9 24.7 25.3 24.9 pH (SU) 8.05 7.75 8.09 7.82 7.94 7.74 8.021 7.6 8.05 7.57 7.98 7.71 8.041 79 7 DO (n ) 7.9 7.8 7.2 7.6 7.8 7. 7.5 7.0 7.8 7.4 8.2 8.

Conductivity pmhosem) 194 143 19 193 193 141 194 Temperture (C) 24.8 24.7 25.1 24.2 25.3 246 25.11 49 25.21 46 24.8l 24.8l 25.4 2.

pH (SU) 8.02 7.73 7.94 7.80 7.90 7.0 7.971 7.651 8.001 7.641 7.961 7.81. 7.99 78 DO (m/L) 7.8- 7.5 7 7.8 7.51 7.9 72 7.6t 7.1l 7.61 7.21 7.91 7.41 8.2 81 Conductivity Qimhos/cm) 7121 5 4 3 4 4 100% Allalinity (mg/L CaCO3)

Hardness (mg/L CaCO1 )

Total Residual Chlorine (m i Temprature C) 24.7 24.7 25.1 24.4 25.31 24.97 25.19 24.87 25.4 24.9 24.8 24.7 25.2 24.9 pH (SU) 1 8.02 7.761 7.961 7.771 7.921 7.741 8.011 7.621 8.081 7.571 7.961 7.781 8.01i 7.89 DO (ffW/L) 7.9 _5 7_8 7.1 80.1 78 70 77 707875B Conductivity qmhos/em) 146 _

IW0% Intake Alkcalinity (mg/L CaCO3)_

Ifardness (mglL CaCO 3)

24. 9r ITotal Residual Chlorine (mJL)

ITemperature cC) ,r .61 25.01 24.21 25.01 24.71 249 247 251 24124. 9 1_4

Environmental lesting S~olutions, Inc. rage a ort Species: Pimephalespromelas Date: I? bq-bc/

Client: TVA / Sequoyah Nuclear Plant - UV-treated Dailv Chemistr:

_ Dsy 0 2 Analyst OA J ]( PA 1- (

Concentration Parameter -

CONTROL pH (S.U.) Sm Soto DO (mglL) -1.4 .e 1 i .*1 15 Conductivity (pimhos/cm) 3 Io Temperature (C) 24z9 Z4.( l 2NA9 24.3 2 . 2 .1 pH (S.U.) 8 O l*s S 0,5e .5 tS.t6 .2 1 10.98% DO (mglL)

Conductivity (pmhos/cm) Z8 Temperature (°C) Zl- Z-& .2L1.2 Z4A3 22° pH (S.U.) 5>X 1 Z -7 - .'&-

22% DO(mg/L)

Conductivity (iimhos/cm)ZoZ Temperature (C) 251.1 27. q .1_ 24.-. S *. .

pH (S.U.) -. _iZ 1.q. IS .% 3 43.9% DO (mg/L)

Conductivity (pmhos/cm) 20 Z5Z Temperature (°C) 2z.e 2 .(, . 29.3 2S. I 21.

_pH (S.U.) q2-05 7I6s.Oo 7Z 1q 72% DO (mg/L) 19 .7.1 Conductivity (ptmhos/cm) Iq41Z - lt Temperature (°C) 7q. 2 Z. 77

2. 253 Zl.

pH (S.U.) g .OZ. -.I A 1.90 1.

100% DO (mg/L) 1.

Conductivity (iimhos/cm)

Temperature (°C) 2Y.1 Z'ti 2S. I 4. 25.3 ZY___

OH (S.U.) ?.e) I 1LL, I i.Q U 1. 1- 1- 2 I-.q 100% Intake DO (mg/L) 1 pi I. 1.7 -7:

Conductivity (gmhoslcm) a Temperature (°C) zS.0 zF 2S.O 0 .

Initia Fi l I Initial I F1 _ Inltlal ll

Winvironmenwai 1 esting Solutinns- Inc. Page 6 ot 6 I:nvironmen[a1 .1eSClni Solutinn. Inc. Page b ot6 Species: Pimephalespromelas Date: 11-6q-ov Client: TVA / Sequoyah Nuclear Plant - UW-treated F1 I Day I II. --

3 I 4 1I 5 I Analyst Concen- Parameter tranton-i CONTROL pH (S.U.)

DO (mg/L)

Conductivity (umhos/cm) t -

Temperature (°C) 9-nD P-MI I I t--,

1% z- I 1. 6a l3 .IZ I 1.66 I .ot' 1.73 I 8.tto ,, ,. . _

10.98% DO (mgtL) 4R.C F - -l.o I__(.11 -I I~L & - 1M~o19 7 Conductivity (pmhos cm) zso ze1 2.69 - zq!_ II, Temperature (°C) W.-7 24.6 241 M 75.O 2S.O ZS53 OH (S.U.) _ R.Z *7.6?I q.1 IZ1.(,3 I &.o04 7.7! ',.to r--x_-_-s . . _ ----

22% DO (mg/L) -1 q I G- 1@

-17 11'.1 '-1-9 -1,5 I F2 Conductivity (1mhos/cm) _ Z(Aq Z67 0 2Z& zs34 Temperature (°C)

. o .

I 72J.-7 I Z5.Z.

II 2. - 4 Zil.5 zS.q pH (S.U.) .%s Lb I B.Iz IB 01 lI.1 % r 1..'

43.9% DO (mg/L) CBO 1.5 .Z Conductivity (Prnhos/cm) IZa 11P-I T Z33 7,O40 Temperature (QC) 2oq.9 Z9 Z5-3 Z -1 '

F 'I OH (S.U.) r \ I ,

1 7_07-SX Iq-L 72% DO (mz/L) I* R.2 Conductivity (Rmhos/cm) I q3 3 187 Temperature (C) 24. C

,r I 'T.L jc4~

s.1 F 4. _ e _ _ _

DH r

(SU.

x_._.s ..... _ -1.q-1 I 1.6! l 4.oo 1l 7.9 I 7A.6 1-i.s6 I 1.-99 l - _,.

100% DO (mgtL) -7 o V I 7-9 __ -1.

Conductivity (pmhoslcm) I ,qq _ 13q 1q?

Temperature (°C) 2zS .1 1 4l. F I Z5.qt I 2.4.¶ Z2.g& 74.1 12T. Z.Z nD (S..II q - t.., 82.01 I172. I '6.08 1 7.57 = 7-q6 1-i. i I z.o i L7.

100% DO (mg/L)

Intake Conductivity )-71 I4q5 _s _. 1g 7.

(pmhos/cm) 2i. 2e1. z.I.SL 9 4 Temnernture PCM

a. -'-,

Iiil Fnsl I Intill Fnal Itil Fnal Initial I

Page T Page k of I Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Co J Iodide reagent: l I 1 Date analyzed [ I-M-OI Acid reagent: 10 p i %

Calibration:

I 0.10 m L I 1.00 mg/L Reference standard number I .%55 Iql I-.*s i'ii Note: For samples with a residual chlorine of > 1.0 mg/L. the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratory control standard:

Reference standard True value (TV) Measured value (MV) % RS MIV / TV X100 number (mgL) (mgfL) (acceptable rnge - 90 to 110%)

(, S i'a.i 0.50 0. 10S.5 Sample number f

Duplicatesample precision:

Sample ID Sample characteristics Residual chlorine %RPD ((S - D) I[(S+D)/2j) x 100 (acceptable range - 10%)

Duplicate 0 D _ _ _ _ _ _

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number (mg/L) s Blank (shouldbe < 010 mg/L) '; 4 --tj l g tY411CR. Vz IAERCL) 5, 16 fALr Yai4, M ?.P-

°>c~ < C4.o~C.4a" q1t4 L I J11q w r- 113 *QijrAC PAMx % 0 iP-r4c.s oo sl oYIMIr.C9 LbgT-lot 1tA- Xc A ELtALJ.uk,, _-nC-tL- r.oc I;QJ.U1 I4 eB L %Q- PAL 't"LJ..& ,eAflTC-L.s < C.) e3 S OLICA.D03 WU Po LTLSV I )-E e Le Qw-or, APThC.L63 _____

O`41108. 07 Soo? I tc iVsE. aPAsr %(LLo .erXTtc.U5 <ce iq -

041l0',.o& kr- -to, PAL q CLEtt _ _0._0 _ _o _

OYIoD.ol St i - 101 L.o . CLAA?_ 4_.____

t) Ic,i O [i - IV, b C.4 L. . CLEA _ ._oo_5_ 3 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratorycontrol standard:

Rererence standard True value (C) Measured value (MV) % RS MVIV / V x 100 number (mgeL) (mg/L) I (acceptablerange 90 to 110%)I

'SS 14I 0.50 OA=')8 t q,1 .U .

Reviewed by ,_

Date reviewed I l-LI -

Page I ° Page I of I Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.10 mnglL Meter: Accumet Model AR25 pH/lon Meter Analyst [Xl lodidereagent: l,. iIZ I1 Date analyzed li -piL III Acid reagent: _I R

I Calibration:

. . I O.lD mWlL I 1.00 mg/L I Reference standard number IvS5 bCLI1 l 5s Aqd Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratorycontrolstandard:

Reference standard True value (TV) Measured value (MV) % RS - I TV X 100

. number (mgI) . (mg/L) (acceptable range - 90 to 110%)

1i45 ,-i 0.50 a 4qL qJ. L Duplicatesample precision:

Sample Sample ID Sample characteristics Residual chlorine %RPD - f(S - D) I[(S+D)12J) x 100 number ____ __ (acceptable range -

10%)

0q8< .5Ml3_ S VAo51 Duplicate : o os I. ___.-

Sample measurements:

Sample . Sample ID Sample characteristics Residual chlorine number (mg/L)

- Blank (shouldbe <0.10mg/L) :P 0.

b 1111. 0 W60ir - PPL- Yguow . O2Le.- 40o.o' 334 D1 I I O0 1 S tJ -tIt PALE YLI( U.tw, Ft'Lu PANtnlLEs st . t6 tA 3

tl -i.

ItzJ LAbIl,1 WILL-C, P PAILZcI.es <O 60 17 S IIII.O1 4 lj3b %-vO' I4A 4 AL ' LLtwe ?A-TrC.LeS _o._O___

0D411 I )-.I L1AV LAUC. LALLJIP qAL.E. 4_t,,J . LS_ 0.LSo_

atl III0 . nCsA tJW1V'uLL°ALL guj.ow. eL L <O.cc-7t 9Y I I .tUt PYT - Its worAy-IL M A YrL{CL-ES E . < O *4S O l.oe.. SOOC4 10^tAfE PALE YULo , PpAr-ic-s <OcZA__

Note: All samples were analyzed inexcess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratorycontrol standard:

Reference standard True value (TV) Measured value (MV) /%RS - MV/TV x 100 number (mgL) (mg/L) (acceptable range -90 to 110%)

I l5$ 0.50 LL5zI 1t. .2 .

Reviewed by l h(

Date reviewed

r-uvironrnental I esting SDIutions, Inc. / -' -5m rage II Page I of -,L Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.1O rng/L Meter: Accumet Model AR2S pH/Ion Meter Analyst II7p7 Iodide reagent: I4. 1-Z7 Dateanalyzed l 11-S. 0] Acid reagent: p4pZ 13S3 Calibration:

I I. 0. =101z/ I .OO mg/L l I Reference standard number 119S01 mgl7 I 1.s0 m l Note: For samples with a residual chlorine of > 1.0 mgtL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Laboratorycontrolstandard:

Reference standard True value (TV) Measured value (NMV) % RS - NIV / TVx 100 number (mgrL) (mg/L) (acceptable range - 90 to 110%)

145i I11 0.50 . IDb.

Dplicatesample precision:

Sample Sample ID Sample characteristics Residual chlorine %RPD {(S -D) Il(S+D)121) x 1DO number (mg/L) (acceptable range 10%)

0012Pt Sat - Io I Tm .IW91 oJAAtkl S ,2.

Duplicate ; D 0 . zi 0.4 Y*

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number (mg/L)

- . -.. Blank (shouldbe=<0.lOmg/L) <r

<n.ooZl bqihizea s r2- ayi C1Q ielloa., Cp& cleb "c.o0255 la,13 U3-2 to 0I 1 o d0 <O.0c09 I6 ogt IIoq -ai LB* II?, kL C Pooci ,.00ooo7(6 s

6'iII2C4 J z. Cleca, i)cJin 00L41def <O.00 1736 O1 Izle. ID wC *,) I l_ Ir__ __- _ __ _ _D. __ __ _6 b q I II2Z ~ nC 0,<kle ~ o13 oqllzeo+eon b - Q~e be~ow r&xktdo V 1< o.D0qz9 Note: All samples were analyzed inexcess of EPA recommended holding tinre (15 minutes) unless otherwise noted.

Laboratorycontrolstandard:

Reference standard True value (TV) Measured value (MV) - % RS - hlV / TV x 100 number (mg/L) (mgtL) (acceptable range - 90 to 110%)

efer1 O 0.50 o. 69 1.P.

Reviewed by t Date reviewed

L~a~aA~jjL~c I C311i~aLg .soiutzons, Inc.

Page I ;.

Page a- of P Total Residual Chlorine (EPA Method 330.5)

Matrix: WaterMDL=0.10mg/L Meter: Accumet Model AR25 pH/lIon Meter Analyst lC.,6 Iodide reagent: [0 17.

I Date analyzed [I ' I -, - 7 Acidreagent: JR 1%

to bracket the chlorine levels of the samples.

Duolicatc samIe Drecislon:

Sample Sample ID Sample characteristics Residual chlorine %RPD - ((S - D)/I(S+D)12iJ x 100 number (mzJL) (acceptable range 1 i 10%)

6`4111-XA LLL Qtox ptdttles

,AV

" n 12-.4iVU SOOt i2. p £ 3 7Aq Duplicate .4*1-14 M

-, Aa IDO-Oooq3Z Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number _mgQ) v Blank (shouldb=c<O.lOmg/L) ' , _________-'__

O)lA z IIIt fj. C? o I or e0-rc, Coooc0o7 Ohcisv c CcA vQe\ou), O Vh!

PiaLJ15.r2 * <..0001 1r1O h rstin- Ca . PONP Ntelkoo., OR tclks <6,0oUZ67 C)LiLSIL5O" S.Ceiz" -u-)uJIoax\Cie e(c40o00Cle t)qIL3.s q C-k p4" 4cle$ <o.oooZ8 Note: All samples were analyzed inexcess of EPA recornmended holding time (15 minutes) unless otherwise noted.

Laboratorycontrol standard:

Reference standard True value (TV) IMeasured value (MIV) % RS IV /ITVx 100 number (mg/L) (mglL) (acceptable range- 90 to 110%)

IN 1 0.50 ga R7.0 Reviewed by Date reviewed

Page b0 Environmental Testine Solutions. Inc. Page .j.of 2_

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [ 7.A ]

Date analyzed LI I-iii II* Titrate samples to pH = 4.50 S.U.

Jand pH of Normality pNormality (A) of H2SO4 Delonized Titrant check D(5 ml Na2 CO s 0.05)/E water reference I standard e 0.251E

= A number ecceptable range - 0.018 - 0.02 Laboratorycontrolstandard:

Reference True value Sample Alkalinity (MV) %*oRS MV/TVI 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO4L) (acceptable range (mg CajCOjL) (ml) ml ml ml -90 to 110%)

Iwss Z-Zq 10 10 100 10 -Z4t~3,q.k 10\- oo toow Duplicatesam le precision:

Sample Alkalinity %RPD -

Sample Sample ID volume Begin End Total Multiplier (mg CaCOJ/L) {(S - D) II(S+DY21) 100 number (ml) ml ml ml (acceptable range -.*10%)

MIA5.);ALtS A~ t 0 f ZZ. 3 Z%.A IO.% 10. i {O 7- get i F ^

^1 Duplicatc JS Z% OZ 4 o31 L4s o'

Matrixspike recovery Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOAJL)

(mg CaCOtL) (ml) ml ml ml _ l Sample alkalinity (B) Measured spike value (VI) % R MV /SV X 00l (mg CaCO,/L) MV - A - B (acceptable range (me CaCOAL) 75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOIL)

IIq O 1S3w I to L

It-50 I ss ~ lAZ-Is C I_

1 I 1-.2 2T 9.' 3 1S I

1_ _ _

S

4. _ _S _ _20 _0.5_1j 2 to .- Z 11-04 17-0 4t13 _ _ _ _ _ _ _ _ t _ _ _ __3_ _

l t _ %W AZt l 3z.1 l 6 o 3.3 1 s I 01040b3 Fjing-P z I $ 13.ol zl1.I 71 Reviewed by: Date Dalereviewed: I I I-1j,-Oq I

Page (i:"

Environmental Testing Solutions. Inc. Page Z of 2 iE::

t;z%:

n) -

Alkalinity i Mo-::

(EPA Method 310.1) t;h Matrix: Water, MDL 1t.rmg CaCO 3/L t; Analyst [ i I ]

w Date analyzed l l-o8 -_Oq Titrate samples to pH = 4.50 S.U.

tLu@:i:.

l -- K 6:

- s -

l :.

M;: S iN Bs l s s--:

r:-l :Ee nfle,.i.-n .#n . . Y.n.-

Sample Alkalinity %RPD -

h;Re Sample Sample ID volume Begin End Total Multiplier (mgCaCOj/L) ((S -D)/(S+D)/J) x 100 number (ml)

v

/'fLo f :

) E.)ItL O Duplicate

\

ml WIieoz)2 ________

ml ml S

D Li (acceptable range - 110%)

- l l i:

ReferencSal le value Spike alkalnity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOIL) r o: Ig(m CaCO)IL) (ml) ml ml ml t_ t t ."

-.,' Sample alkallnity (B) Measured spike value (MV) % R-WMV l SVx 100

', (mg CaCO,/L) MV - A - B (aiceptable range

,. (mg CaCO,/L) 75 to 125%)

':4",'

t'- Sample measurements: _

Sample volume Begin End Total Alkalinity

' . Sample number Sample ID (ml) nml I ml ml Multiplier (mg CaCOJL)

'S ' '

b'IIDob.P3 lN l~ 3 _l _____ Z.3.4 12,. 4o lol

e NII 0VI P-V. F-L *'r 1N IZ-44 213e4 40s lIb 405 4ir2 Sl,-' .

.. O '4itoz. OSt-SA o_______oz. -~ Igu eU 4vs

, e. K - ____ NO-Z S1 3 -- 11 3so7.

'A' 1's-.

,_X- Io-ZlO-4 SA LI A pO I.1 I -q -%.I:>

1h-01-Ct4 SA-r l l l 6__ l 13._ 13.3_I_l13O

).t, II-Os-04 ISALT t1iI 1l3,1 pms, 1.3( I ll hi' ta .-

,'s, '

%%-O~-i-o SAL:r i&O- I I IZ.3 41.,o 34 I 4 A"

o.:as' ' Reviewed by: lIA Date D reviewed: 5 I-IFOq -- ]

>v

'te t.4.

'W. ':

Page 10 C Environmental Testing Solutions. Inc. Page I - of-*__

Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO 3 IL Analyst Date analyzed [ Titrate samples to pH = 4.50 S.U.

Titrantno mality and ,nuldiplierdetermination:

pH of Normality Normality (AN) of H2SO4 pH Factor or Multiplier Delonized Titrant check Begin End Total (5 ml NazCO 3 x 0.05)1E - (Nx 50000)/100 ml sample water reference standard ml ml ml - 0.251E - N xs500 4.5 S.U. number number (E) (acceptable range - 0.018 - 0.022) i-3 vie1J 71 I JJ IOS 0.0 12-5 IZ _ _ O. O____5 __b_

Laborato controlstandard:

Reference True value Sample r Alkalinity(MV) %RS-MV/TVx I0 standard number (TV) volume Begin End Total Multiplier (mg CaCOIL) (acceptable range (mng CaCO31L) (ad) nil ml ml 90 to 110%)

IM-55 Z24 100 100 12.5 ZZA6 q5 t). Oil q-7 /

Duplicatesam ple precision:

Sample Alkalinity %RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCOjlL) U(S - D) II(S+DY21I I 00 number (mi) ml ml ml I (acceptable rnge -. +10%)

\lk-(AD InA lAOC14 tZ 1 72.28 Iwt 2_o (5 W r-;:!

Duplicate _Z

__D .

Matrix spike recovery:

Reference' Spike value Sample l Spike alkalinity (A) standard number (SIV) lvolume Begin End Total Miultiplier (mg CzCOj1L)

(mgICzL) (ml) ml ml ml III w z55

,Zaq 5o Z XS i.5t 1I AE III l Sample alkalinity (B) Measured spike value (MV) % R -MV/ SVx 100 (mg CaC.Oi/L) MV - A - B (acceptable range I (mg CaCO9L) 75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml nml Multiplier (mg CaCOlL)

__-0__ _ I 5 fD0 AZO 10t: .Q LK'S k,L. tO

_ 0-__

___ h 1 I&O I _ JtoAJan .bl1 I

^o )is >Sn12 as.O V&~ 1 t 1 b 41 C, l ln$-1. 12e7 5 l 59 l lSS l LA3A l l41 12,1 54 34-Rl-i+4- 9 0il}2-0 -fl AV, 1t&. 1z113f

_O jjO8-025 WAY4VESVITLT I a_lI l5-1 1_@. 1 \ It l 0110-tCA It Z 7+ 3 -i-315 I L-3 l I'sl Reviewed by: 1 De Dale rreviewed: d I 11-18-0T I

Page 7 l Environmental Testing SuntIfinns. In.c. Page Z of 4L F' Alkalinity I'.. Analyst CA)

(EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO 3/L I.: Date analyzed l

.I lirarzn 4 l 2-64 rulIdplier dtec rmination:

Titrate samples to pH = 4.50 S.U.

I pH of Delonized water Titrant reference Norniity check standard Begin ml End ml

__Normality ml (N) of H2SO4 5ml Na 2COj x .0OS)/E 5N500J

=

pH Factor or Multiplier (Nx 50000)/100 ml sample 4.S S.U. number number _ (E) (acceptable rang e - 00 _r-V.I;,

Laboratorycontrolstandard:

Reference True value Sample I Alkalinity(MV) %RS"=MV/TVx1OO standard number (TV) volume Begin End Total Multiplier (mg CaCO3tL) (acceptable range (mg CaCO3L) (MD ml ml ml -90tol10%)

zs zz4 100 100 '31-5 LO1.L q1 b.0 Ilqn'.

Duplicate sample precision:

I Sample number Sample ID Sample volume (ml )_

Begin ml End ml Total ml Multiplier Alkalinity (mg CaCOj/L)

%RPD

((S -D) Il(S+Dy21) x 100 (acceptable range -

10%)

1. IS Duplicate 4- -

I. l Matrix spike reco vq Reference standard number

~

Spike value (SV)

(me CaCOA.)

Sample volume (M

Begin ml.01z End ml Total ml Multiplier Spike alkalinity (A)

(mg CaCOAL)

.j Zzt 7,-i5 6 0 10lb l [t %0-o -10I.0z l Sample alkalinity (B) Measured spike value (MV) % R MV I SV x 100 (mg CaCOAL) MV-A-B (acceptable range (mngCaCOAL) -75 (o 125%)

Samlemesuements:

Sample volume Begin End Total Alkalinity l (ml) ml ml ml Multiplier (mg CaCOsL)

IA Sample number Sample ID 16.aq 01 501 LI 192 D LO Ito. 5. it.o Ii 41

' 411

______S____IQlzo Z0,of7 IzL,4 51:1 zim, 124.01 5. Z 1 5_ S I 1 1 F1 So oq011 8.07- 4 3 - -

OL01 0 11 /lo 113.g.

01 1 32I I __ I_ 1. l1.n l b l _ _

_ _ _ _ __T_ _ _

Reviewed by: Ll iIAs I Date reviewed: L1-i E04 -Z

K Page JZ Environmental Testing Solutions. Inc. Page 3 ofj i U

I Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3IL Analyst [ E Z V Date analyzed pH of Sitrnt nr i lk_,S I tuplerdeJtermination:

Normatlet_

Titrate samples to pH = 4.50 S.U.

Normality (NM of HIS04 pH Factor or M~ultiplier Delonized Titrant check Begin aULA_ (5 ml N22CO3 s 0.05)IE (N x 50000)1 100 ml sample 12 water reference standard ml ml ml - 0.25/E _Nx s500 4.5S.U. number number (E) (acceptable r3nge A1-O022)

I: Laborato controlstandard:

Reference standard number True value (T,)

Sample volume Begin End Total Multiplier Alkalinity (MV)

(mg CaCOJL)

%RS1-MV/TVxlOD (acceptable range S

(mgCaCO31L) (MD ml ml ml -90to 110%)

IM55 ZZ4 100 100 1-. 3' .- l /DO. 1 Du licatesam le precision:

IL.. Sample number O'4 I)OBct Sample ID jJ 16i 1Pr, Sample volume (ml)

Begin ml A$j End ml 3 .S 53 Total ml Multiplier PWX Alkalinity (mgCaCO,/L)

%RPD -

J(S-D)I[(S+D)121 x I 00 (acceptable range - t 10%)

Duplicate 4 I- 32 W53 5 D 53 .

I. Matrix spike recove Reference standard number Spike value (me (SV)

Sample i volume CaCOstL) l(ml) lml Begin End ml Total mll Multiplier j Spike alkalinity (A)

(mg CaCO/IL) l 1.... v6ASS Z- 60) l t 10 Sample alkalinity (B) 32.3 4Z41 iti-Measured spike value (MV)

)0 1l I~e7

% R =MV ISV x 00 (2Ceeptble rnnge l

F (mt CaCOAL) l .MV - A-B l l l (mg C&CO..tL) -75 to 125%) l Sample volume Begin End Total Alkalinity Se S l ID (l Multiplier (mg CaCOL) l l_Oq# 11. OgI Wd tj Wr ttl 7-Z l 3.3 __lq.- A 5° aD 5q I OQ§Io I .3 .1 I I-7 1 b-171. 1 61zo >

0z411l1.{028- I1 - Z1 - 2%*t I II I 51 5,

0!llt 11 I O-J 2. l l,. m -4.ls{n. l L C4IIII3Oi (n I us ll 3&o S2 1tv-S 6to

( 1 tIIq.if wBO tt3 W6Js. 1 N tiiS-1 Reviewed by: Date Da reviewed:

r e d::]I 11-*Oq 71

Page *13 r

Environmental Testing Solutions. Inc. Page '1 of L I Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3 /L I frrmt-pH of Analyst Date analyzed it-I S

!plier Normality l

determination:

Titrate samples to pH = 4.50 S.U.

Normality (A) of H2S04 pH Factor or Multiplier Delonized Titrant check Begin End Total CQ x 0.05)/E - (Nx 50000)1 100 ml sample

.4U: water reference standard ml ml ml -0Nx50

4. 5S.U. number number =_- () (acceptable range -0.018- 0.02)

Laboratorycontrolstandard: a Reference True value Sample Alkalinity (MV) % RS - MV/TV*x 001 standard number (Tc) volume Begin End Total Multiplier (mg CaCOA/L) (acceptable range (mg CaCOj/L) (ml) ml ml ml -90 to 110%)

l9sS zz.f 00 1 100 3ul 4Z3 9. lb, cI-z/.

Duplicate sam ple precision:

IA.. Sample number Sample ID Sample volume (ml)

Begin ml End ml Total ml Multiplier Alkalinity (mg CaCO 3IL) U(S

%RPD -

- D) IU(S+D)r2II x 100 (acceptable range -* I0%)

Og4IL.OI. 0 RS i. Z J6 b,_-_ 10.0 \ol 1_ _ _____1 1t Duplicate _ t (EP I0 (lC It IDI4 /

M atrix spike reco er :_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

l Reference Spike value Sample Spike alkalinity (A)

I standard number (SV) volume Begin End Total Multiplier ((mgCaCOIL)

I (mgCaCO3/L) (ml) l ml ml ml IOcss Zzq I 0 )OD 8W.@lz.4 IL.1 l' (D.0 167 ' JW I1 II l l

I Sample alkalinity (B)

(mg CaCO3IL)

Measured spike value (MV)

MV - A - B (mg C&CO,/L)

I I

% R - MV I SV x 100 (acceptable range 75 to 125%)

Sample measurements:

Sample volume Begin End Total Alkalinity I Sample number

-64 II Is o II4BI5 Sample ID 113 LUT 3 l (ml) lbo IZ*

ml ml 33-3 58 ml Multiplier IC D (mg CaCO,/L) 59 I IOulIII)O GLpuIOCI o9 IM-t1 4-lQyous

'E

\

_0.3_13.1 I l Ss lts.0 II s 1 1S.St, Z'L 2.1.2-1 Z.L Lt 1 1 L11 10 Reviewed by:

EZJ I Date reviewed: I IlI--I o i

I Environmental Testing Solutons. Inc.

Total Hardness Page Page

( of CoD Z.

(EPA Method 130.2)

Analyst [ 7.77m d Matrix: Water, MDL = 1.0 mg CaCO3/L 3]

Date analyzed - *kTlL l ok1 dd2 4 !d I C-7C HM.. 1q81 trant nomality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E (Nx 50000)1 50 ml sample number number (E) (acceptable range - 0.018- 0.022) - N x1000 JOIvL25 U65s 1a-72 O0. Q.4 '9 O7 Z4. Z

?:. Laboratory control standard:

Reference True value Sample Hardness (MV) % RS =MV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOtL) (acceptable range (mg CaC0IL) (ml) ml ml ml -90 to llO%)

IMS5 1I1C0 40 50 jc Iq. Z.o ZO0q:lKl Duplicatesam le precision:

%RPD I

Hardness =

Sample Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) U(S

-D) Il(S+DY2 1) 1100 number (ml) ml ml ml (acceptable range - 10%)

I'2I 4

4 Duplicate 4_ _l_. _ 44 1 D_ _

Matrix spike recovery:

Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOAt1) l(mg CaCOVL) (ml) ml ml ml t Ito0AC- So IL.0 Z. lp. Z.Z 12 I

Sample hardness (B)

(mg CaCOA) l Measured spike value (MV)

MV= A- B (mg CaCOIL)

I % R MV I SV x 100 (acceptable range 75 to 125%)

447.

I:. Sample measurements:

Sample number Sample ID Sample volume (ml)

Begin ml End ml Total ml Multiplier Hardness (mlg CacoIL)

Blank

-s (shouldbc-OmgCaCOA) l lc) OCo OXl o -?Z 1IImC z rn~s AZO -A l_ } lz2.z, I.3 4-t l 3as, 11-ts

__ _ _ _ IW \:c I IZ J~ l3 4.1 1 it-.

l\-oS-oP IsstwiMzo I 1A I~ l^ t2 1~ 1~-s F 1t-O1-o'4 wliS IMzO - ___ l *~t 1l1 1 -. 1 8 1_

_ _ _ l _ _ _ _ _ -s l _ _ _ l.v lE t +/-;I l l-I o'-i 1lb~ i 1 , 11 - 2*- -1

_l1 F 1 I

Note: If >15ml of titrant is used, sample must be diluted.

by. -*

cur";"

&.afL JVA

. 5 CA ,

Reviewed I Il Date reviewed LIP-il gLor..L ks So~r

Page Lto Environmental Testing Solutions. Inc. Page Z of Z.

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed I aJ I i(-C-I4C d

_ itrant no ination:

Titrant Normality check Begin End - Normality

_ (N)of EDTA pH Factor or Multiplier reference standard ml mil ml . .21 E = (Ns 50000) 50 ml sample number number (E) (acceptable irng . .022) -NI 1000

'4 Laborato controlstandard: e4 I Reference standard number True value fTV)

(mg CaCO3/L)

Sample volume (ml)

Begin ml End ml Total ml Multiplier Hardness (MV)

(mg CaCOjIL)

%SRS-"MV/TV s 10 (acceptable range 90 to 110%)

v6 40 50 V-03 Zv &+lOj}

I Duplicatesample precision:

1. .wt Sample number toc/:

if Sample ID EA, Sample volume (ml)

Begin (a

ml 3.

ta B End ml

° Total ml Multiplier Hardness (mg CaCOjL) z F;

%RPD -

((S. D)II(S+DY21) x 100 (acceptable range - k10%)

~ ;,

D411t 3 Duplicate I IL U_ 1 IDbl_1.

Matrix spike recovery:

Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CsCO/L)

. (mg CaCO3/L) (ml) nl ml ml 435ss I to '40 50 11. 1 I Z5 .- 1

-;i*..

- 2 Sample hardness (B) Measured spike value (MV) % R=MV/ SVx 100 I I Sa(mg CaCO/L)

I MV- A - B (mg CaCO L) j

. I (acceptable range 75 to 125%)

I Sample measurements:

Sample number Sample ID 34 110Z Sample volume (ml)

Begin ml End ml Total ml Multiplier

/.

Hardness (mg CaCOL)

I be - mg CaCOL) -(should -

C.-..

IQ4110M.0N I3tgosw-U 420-0 T2o T- T I X 61 -I 41i02z45 jIn LP PAPCf T Z3.o z -Ls 4 I-i AD IA_ __l_S l 5l

~~AI I 11 Note: If >15ml of titrant isused, sample must be diluted. Reviewed k7 by: I kl DatereviewedI l *aA I 14

Page l Z.

Environmental Testing Solutions, Inc. Page I of .

Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3yL Analyst Date analyzed Li-1 j-EA j Titrantnormality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E (Nx 50000)15 0 ml sample number number (E) (acceptable range - 0.01 . 0.022) -NX 1000 iX i7Z 7 I .q O-oZ7oa Laborato control standard:

Reference True value Sample Hardness(MV) %RS=-MVITVxtOO standard number (TV) volume Begin End Total Multiplier (mg CaCO3 IL) (acceptable range (mg CSCOAt) (ml) ml ml ml -90 to I10%)

lXs lto: 40 50 P q1.92*o *'ZO.Z 4c.

Duplicatesamiplerecision:

Sample Hardness %RPD -

Sample Sample ED volume Begin End Total Multiplier (mg CaCOJL) ((S - D) tl(S+D)/2l s 100 number (ml) ml ml ml _ (acceptable range - 10%)

1\-O9-0'4 rA1s 1Z-b 510 IUCI Ui0.0 Lt.l Z, a S 2 ,:

1 Duplicate D Matrir spike recovery:

Reference Spike value Sampe Spike bardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOjIL)

(mg CaCOVL) (ml) ml I ml ml INS55 lucb 4o St Ilio IZ?-t 1.l to ZO.L 1s3 Sample hardness (B)

(mg CaCO3IL) l Measured spike value (MV)

MV - A - B (mg CaCO31L)

I % R - MV / SV x 100 (acceptable range 75 to 125%O/)

Samptc measurements::

Sample volume Begin End Total Hardness Sample number Sample ID (ml ml ml ml Multiplier (mg CaCOA)

Blank 0.0 (- aC l________ l(shouldbe-OmgCaCO/L) 6 00 0.0 ZO.L 70I ln4Y5 160iC A - 72. ? '-tl T_,_1___

1- 8 44 03 1 1 14 136.3a

l 1 t

{\\-\z-s ~ - 1 10324 11 It 1 t S l S5 IL2.b A 2 i2L44. AL l 2- 4 Z_42 T

l__t-t_-b_ lh 1A5 Fb l __ _ -1__AS.R 4-k+/- - -

lla3C NtWAY1E5V1LkJg OF l l L3 o~LIO2.01LkAYEsM5iLU I I 5) 1.j3 15 is.4 _ ______

11-18 I Note: Tf >15nl oftitantis used sample mustbe diluted.

by:

Reviewed I 1I Date reviewed i

Page &k3 Environmental Testing Solutions. Inc. Page Z of - &P Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3 JL Analyst [ 7 iI ]

Date analyzed Ttrant normaliantermination:

Tltrant Normality check Begin a al Normality (N) or EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E (Nx 50000)/50 ml sample number number (E (accept0.08-0.022) -NX 1000 Io

... Laboratorycontrolstandard:

Reference standard number True value (TV)

Sample volume Begin End Total Multiplier Hardness (MV)

(mg CaCOilL)

% RS - MV / TV x 100 (acceptable range (ml)

I. RoUlb (mg CaCOA) 40 50 ml 15s4 1A ml ml 20.z 4.

- 90t0 110%)

IC)O i Duplicatesample precision:

Sample Sample ID Sample volume Begin End Total Multiplier Hardness (mg CaCO.L)

%RPD

((S - D)/I(S+D)1211 x o00 number __ ml) ml ml ml _ (acceptable range - t 10%)

1. o-4 t Ilo.0C)A 4 b Duplicate L&Z.1.

4jq 2j 3

L5t zc'z s D

i~zC) 1' Matrix spike recovery:

Reference Spike value Sample l Spike hardness (A) l standard number (SV) volume Begin End Total Multiplier (mg CaCO/L)

(mg CaCO3IL) (ml) ml ml ml l)S55, POO ZC 50: 2AP,9 3T.41 _U.,_ 2DZ § Z 40 5b 4.~ 2 oZ Sample hardness (B) Measuredspike value (IV)l % R- MVI/SV x 100 (mg C&COJ/L) MV - A - B (acceptable range (mg CaCO/L) - 75 to 125%)

I I.1 Ilb Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOL)

I.. _

Blank-(should be -0mg CaCO)/L) l_

-s

_ ___lC_ _

I.. 106l Ic.0 LZ i9lY 4 'jai LAu 3ISsH SG Li I 15O I ;5t,2

5. ta

'5

%(C (A___

I I T- 4 I

.... 04-1 Ii Ot4 IIQ. OL-

, 2..I N 10&-ot'tx S~OO -hrr,4V-C I I Z 3L T I I

tz I z%

Iq.Z. 1 q lz4s ZA I T

-q 1 - T 54 sq IIfib .ow 7fi 2zsl0AZ- 5 2.el: 5+

OLL 11i. OK L9WA" 11 I ___ - 3 3.7. W.. leg 0 1"It. 1 41

- 2 33_j___ 3___1 _ 69 Note: If >15ml of titrant is used, sample mustbe diluted. Reviewed l 8\ l Date reviewed l by:

Page lo/r Environmental Testing Solutions, Inc. Page 3_of !t Total Hardness (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO 3 IL Analyst [7 7 l Date analyzed 11I57qII1 7ra n n determination:

Titrant Normality check Begin Normality (N)ofEDTA pH Factor or Multiplier I

End reference standard ml ml ml E (N x50000)150 ml sample number number l (acceptable rnnge . 2 -Ns 1000 Laboratorycontrol standard.

I. >sS Reference standard number lwb True value (TV)

(mg CaCO/L) 40 Sample volume (ml) 50 Begin ml 37.O Sq.0 Z.O End ml Total ml Multiplier Zo.2-Hardness (MV)

(mg CaCO3IL)

%RS-MV/TVxIO1 (acceptable range

-90 to 110%)

loo it Duplicatesano le precision:

Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (nm CaCO,/) ((S - D)t[(S+DV21) x 100 number (ml) ml ml ml (acceptable range - 10%)

IL.. [

o'111o1 Ia.60 Duplicate

1. 1 3 5O 3 I o 44Z 5

_3 2 S D Z7

/

Matrixspike recovery:

I Reference standard number Spike value (Sv)

(mg CaCO,/Ll)

Sample volume Begin ml End ml Total ml Multiplier Spike hardness (A)

(mg CaCO/L)

I35S5 Sbb ) 1 5.1 7-O-Z-l Sample hardness (B) Measured spike value (MV)

I I I

(mg CaCOjtL) MV - A - B (mg CaCOVL)

I %R -MV/ SVx 100 (acceptable range 75 to 125%) I I Sample measurements:

Sample number Sample ID Sample volume (ml)

Begin ml End ml Total ml Multipiler Hardness (mg CaCOIL)

B lank _ _ _ _ _ _ _

(should be -0 mg CaCOX/L) _ _ __ __ __l_ __ _

IILQio 11oJ IC, WT. i 5 0AL.43S A2 4 2if1{ .- It_ _ 3 .V CI

,CLU1cA. 0 -1 LO is~ 11Z 1&ii k L I

O1IIif. O T___ $. Z . - -4 041l[3-6(a .3 1 24.0 29.r1 41 q35-l I lotLlb O L I IORvbf .J i.5is A.I WW At II 113 1 3hI 1 l?.- % R13z55.3 13.1 S.. L l l 1

l 19_

l3 it l I3 I pqi Jl 3.09l + 3_

Note: If >15ml of titrant is used, sample must be diluted.

Reviewed

_ _ 1 l <

Date reviewed lFfl9O7J

_ _ 1_ _ _ _ __8 1

by: LIZA

Page &5 Environmental Testing Solutions, Inc. Page 4 ofC4 Total Hardness a Analyst e 7j7 (EPA Method 130.2)

Matrix: Water, MDL = 1.0 mg CaCO3/L I Date analyzed 11t-5 1ultiplier determination:

IF.

I. Titrant Normality check Begia _Total Normality (I) of EDTA pH Factor or Multiplier reference standard ml ml ml 0.2/E (Nx 50000)150 ml s~mple number number (E) (acceptab e-r- I - 0.022)0 Laborator controlstandard:

Reterence True value Sample; CHardness (MNV) % RS - MV I TV s 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO,/L) (acceptable range

.(mgCaCO.) (ml) ml ml ml -90 to 110%)

100 40 50 k.S 2-Z L 2 to*/.

IA.

I05S Duplicatesam le precision:

Sample Hardness %RPD -

I:. Sample number 04l tI11 l.1 I Sample ID

&il I3ASaT. 5b volume (ml)

Begin ml End ml Z 2D652. 5°1 7-q Total ml Multiplier 2:)6.,

(mg CaCOIL) (S -D)4(S+Dy211 x 100 (acceptable range -10%)

_ Duplicate 4- Z3.4 2>#3 IZq 1 l D 51 I, Matrix spike recor Reerence standard number l evl Spike value (SV)

( SgV )

Sample volume v(ml) ue Begin End Et_

Total Multiplier Spike hardness (A)

(mg CaCO,/L)

_ _ml

__ml_l _ml I 1)3slT' too t I 6,p 1 5CJ IZ& t.'I Z.Z l Sample hardness (B) Measured spike value (MV) % R -MV ISVx 100 I: (mg CaCO,/L) - MV - A - B (mg CaCOIL)

(acceptable range

-75 to 125%)

1 ~59 16I 10t I Sample measurements:

Sample number Sample ID Sample volume (ml)

Begin ml End ml Total ml Multiplier l Hardness (mgCaCO/tL)

Blank-I l ______ _ l(should be -0mg CaCO,/L) l _ _ _ _ __.

nlO 113 1U. 21 doL2.- t 3 6p. -SQ53 2oa. il dSflJ8...L ... 1 31___ 4 13dl°134 3.3 1 + I I 5,..

1*

L Note: If >15ml oftitrant is used. sample mustbe diluted.

by:

Reviewed l 1 41 J Date reviewed I l!jZ-Oq I

Sequoyah Nuclear Plant Biomonitoring November 9-16, 2004 Appendix D Reference Toxicant Test and Control Chart

Environmental lesting Solutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.0 I , , , , ,.. , ,-- , , , I I I I ' ' 'I 0.9 State and USEPA Mandated ControlLimits Limits Set According to i 2 StandardDeviations 0.8 0.7 -. . . . . . . . . . . . .. . . . . . . . . . . . . ............... . .. . ................ ... . .. . .......

0.6 00,11%

9-"

0.5 _ ~......... ........... .............. ............. ...........

U W

0.4 k-19 kn I I I I I I I I I I I I I T - I I I I I I N 1.0 U

-q

>4 0.9 USEPA Warning and ControlLimits Po Limits Set According to 10 h and 25 :h Percentile CVs I

t- 0.8 0.7 0.6 0.5 _................................................................................................................................................

0.4 Test date

- 7-day IC 2. = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.

- - Central Tendency (mean IC2 3)

Warning Limits (mean IC2 SA.10 )

. Control Limits (mean IC25 SA.25 or 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water State and USEPA Laberatery labenrtery 1UTSEPA USEPA Test number Test date 7-day ICan CT S Control Linits S'U* Wmind Lintts SAU Central LUmits SAM war ning Lfimts SA Central Limits Cv (IL KCI) (gLKCI) CT-2S CT+2S CT-SAIe Cr+SA,, CT-SAS5 CT+SA2S Cr -SA 7S CT + SAM CT- SAn. CT +SA 1 04-13-04 0.64 2 04-20-04 0.63 0.63 0.01 0.61 0.65 0.03 0.56 0.71 0.13 0.50 0.77 0.24 0.39 0.87 0.29 035 0.92 0.01 3 05-06-04 0.63 0.63 0.01 0.62 0.65 0.03 0.56 0.71 0.13 0.50 0.77 0.24 0.39 0.87 0.29 0.35 0.92 0.01 4 05-11-04 0.64 0.64 0.01 0.62 0.65 0.03 0.56 0.71 0.13 0.50 0.77 0.24 0.39 0.33 0.29 035 0.92 0.01 5 05-18-04 0.56 0.62 0.03 0.56 0.69 0.07 0.55 0.70 0.13 0.49 0.75 0.24 0.39 0.36 0.28 0.34 0.90 0.05 6 05.18S04 0.5S 0.62 0.03 0.55 0.68 0.07 0.54 0.69 0.13 0.49 0.74 0.23 0.33 0.35 0.23 0.34 0.89 0.05 7 06-04804 0.61 0.61 0.03 0.55 0.68 0.07 0.54 0.69 0.13 0.49 0.74 0.23 0.33 0.85 0.28 0.34 0.89 0.05 3 06-22-04 0.57 0.61 0.03 0.54 0.67 0.07 0.54 0.68 0.13 0.A 0.74 0.23 0.33 0.34 0.27 0.33 0.88 0.05 9 07.07-04 0.54 0.60 0.04 0.52 0.63 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.31 0.83 0.21 033 0.7 0.06 10 07.13-04 0.55 0.60 0.04 0.52 0.63 0.07 0.52 0.67 0.13 0.47 0.72 0.23 037 0.32 0.27 033 0.86 0.07 11 07-20-04 0.52 0.59 0.04 0.50 0.63 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.81 0.27 032 0.35 0.07 12 03-10-04 0.56. 0.59 0.04 0.50 0.67 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.31 0.26 032 0.35 0.07 13 03-11-04 0.64 0.59 0.04 0.50 0.68 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.32 0.27 0.32 0.36 0.07 14 08-17.04 0.62 0.59 0.04 0.51 0.68 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.37 0.82 0.21 033 0.36 0.07 15 09-14-04 0.57 0.59 0.04 0.51 0.67 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.37 0.32 0.27 033 0.86 0.07 16 09-21.04 0.64 0.59 0.04 0.51 0.68 0.07 0.52 0.67 0.12 0.47 0.72 0.23 037 0.32 0.27 033 0.86 0.07 17 10-12404 0.61 0.59 0.04 0.51 0.63 0.07 0.52 0.67 0.12 0.47 0.72 0.23 031 0.32 0.27 033 0.36 0.07 13 10-19-04 0.52 059 0.04 0.50 0.63 0.07 0.52 0.66 0.12 0.47 0.71 0.22 . 0.37 0.32 0.27 032 0.36 0.07 19 11-02-04 0.63 0.60 0.05 0.50 0.69 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.36 0.08 20 11-09-04 0.54 0.59 0.05 0.50 0.69 0.07 0.52 0.66 0.12 0.47 0.72 0.23 0.31 0.32 0.27 033 0.86 0.08 Noe! 7-d IC,5-7 y25% ihlbkin cocentratkn.n An etinatimn of tie concetatio ofpobauim ehkride lsttwould csase a25% reduclik nPinphe gwvth forthe testpopubrtm CT Central tendency (mean 1Cjj S - Standard deijt of the ICs values.

Laboratory Contral and Warning Limits Laboratoycstroland waging ii saished usingthe sndard dettib ofthe Ci nvalues coirrepondingto the lOth and 25th prcentile CV c twer These rangs ae mor strigenttn te ontmltndwaninginks recommended by USEPA for the test method and endpoint SA ,.-Standard deratii corresponding to the 10' percentile CV. (SA.19 - 0.12)

SA - Standard devation correponding to the 25' percentie CV. (SA, - 0.21)

USEPA Centrl and Warnin Umits Standard deviation corresponding to the 75' perentc CV. ( SAU5

--A§ - 0.33)

SA." - Standard deviatin corresponding to the 90' percentle CV. (STo - 0.45)

CV - Coefli*ient of amrtion of the IC- values.

USEPA 200Q I andktgand AccotktgfrtMhod Vaebly Whole EltTo kly latksUs ida theNa imhlnhtDschars a*2tbro1i EPAr431-.R.. Ut Env blmopteedbASmryncl , OR1L Orte.fuuu Otskadftom Aqu At BroSptewu.

eSic Inc I I0944

bnvironmentai lIesting iolutions, Inc.

Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.0 I I I I I I I 1 I I I I I I I I I I I I 0.9 0.8 t 0.7 0.6 ................ -- *.................................. .........................................

U 0.5 0.4

_ u ----- . ------

0.3 I I I I I I I I I I I I I I I I I 1. 1 1 0.2

°2"&°N QS %Sv0-X "tXaO oy, tzOX SwS 0 %*:,

vS0 s \0 t S %,9N e9rO N0zN N0cN %. Nv Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

- - Central Tendency (mean IC2 5 )

- -- Laboratory Warning Limits (mean IC2 5 S1: SAJO ISAJO = 0.12)

.......... .Laboratory Control Limits (mean IC25 4 SA2 5, SA25 = 0.21)

- - USEPA Warning Limits (mean IC25 SA.75 I SA.75 = 0.38)

- USEPA Control Limits (mean IC25 + SA.90, SA.90 0.45)

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean CT CV CT MSD PAISD CT Survival Growth

(°) (mg/larvae) for Control Orowth (0f) for Control (%) for PMSD (0/%)

(Mg/larvae) Growth CV (%/)

1 O4-13-04 100 0.780 9.8 0.11 13.9 2 04-20-04 100 0.784 0.782 5.5 7.6 0.08 10.2 12.1 3 05-06-04 100 0.832 0.799 5.5 6.9 0.10 12.2 12.1 4 05-11-04 97.5 0.655 0.763 13.1 8.5 0.11 17.1 13.4 5 05-18-04 100 0.718 0.754 4.4 7.7 0.08 10.5 12.8 6 05-18-04 100 0.681 0.742 9.3 7.9 0.12 17.8 13.6 7 06-08-04 100 0.859 0.758 1.3 7.0 0.08 9.6 13.0 8 06-22-04 100 0.700 0.751 15.1 8.0 0.12 16.8 13.5 9 07-07-04 100 0.610 0.735 11.1 8.4 0.08 13.6 13.5 10 07-13-04 100 0.805 0.742 10.2 8.5 0.12 15.4 13.7 11 07-20-04 100 0.727 0.741 15.6 9.2 0.17 23.4 14.6 12 08-10-04 100 0.490 0.720 11.6 9.4 0.07 14.7 14.6 13 08-11-04 100 0.673 0.716 3.6 8.9 0.12 18.4 14.9 14 08-17-04 100 0.682 0.714 7.4 8.8 0.10 14.4 14.9 15 09-14-04 100 0.825 0.721 12.9 9.1 0.13 15.4 14.9 16 09-21-04 97.5 0.700 0.720 15.8 9.5 0.12 16.4 15.0 17 10-12-04 100 0.759 0.722 12.9 9.7 0.17 22.1 15.4 18 10-19-04 100 0.757 0.724 1.1 9.2 0.09 12.3 15.2 19 11-02-04 100 0.756 0.726 10.9 9.3 0.15 19.3 15.4 20 11-09-04 100 0.609 0.720 7.8 9.2 0.11 18.0 15.6 Noie CV - Coefficient of variation for control growth On average, the CV for control growth is 9.2% in Environmental Testing Solutions, Inc. Pimephales chronic toxicity tests.

Lower CV bound determined by USEPA (l0epercentile) - 3.5%.

Upper CV bound detetmined by USEPA (90' percentile) - 20%

MSD - Minimurn Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimun percent difference between the control and treatnent that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occUrs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 15.6% from the control LowerPMSD bounddetenninedbyUSEPA (l0'percentle) - 9.4%.

Upper PMSD bound determined by USEPA (90e percentile) - 35%.

CT - Central Tendancy (mean Control Growth, CV, orPMSD)

The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.

USEPA 2000. Urderstanding and Accounting for Meffiod Variability in Whole Efluent Toxicity Applicatons Under the National Pollutant Diseharge Elimination Program EPA 833-R-00-003. US Environmental Protection Agency, Cinciemati, OHI Organirmsobtainedfrom Aquatic BioSystems, Inc. 11-09044

Environmental Testing Solutions, Inc.

PimephalespromelasControl Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 I I I I I I

I I I I

I I

I I I I I I I I 1.25 [

_ U 1.00 I o es .. . . . . ................................... ............... ............ ..... ......... .... . . . . . . .. . . . . . .

F-

-1..

0.75 U

o .,c 0.50 .

0.25 Minimum Acceptance Criteria (0.25 mg per surviving larvae)

I I I I

I I

- I

- I I I

I I B I T T I T I I T I B I 1 1 1 1 40 North Carolina Acceptance Limit (< 40.0%)

X ea

_ F.

9 I 30 Kentucky Acceptance Limit (< 30.0%)

_ I o0

._ S 20 1 O 0

..UU L-

-U S. 10 W ........................ ...... I - I - -

V 0 Test date

Control Growth or Coefficient of Variation (CV)

- - Central Tendency (mean Control Growth or CV)

.. Control Limits (mean Control Growth or CV 4 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water l I l l I I I I l l I I I I I I I I I I 40 30 0"'

U) 20 10 ........................................................................................................ ..........................................................................................................................

0 tkNt' ^SS° t09° to '° ~ zo;' °9 oN '° z1v° 1 o'°1pr %s-" wl\ '° ^, &1`° 9,b ,°our* o,9 oOo, Test date 0 PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

- - Central Tendency (mean PMSD)

. --- Lower and Upper PMSD Bounds Lower PMSD Bound (IOth percentilc) = 9.4%, Upper PMSD Bound ( 9 0th percentile) = 35%

(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)

Environmental Testine Solutions. Inc. Page I of 5

_niomna Tetn Souio_nc Pag 1_f Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas PpKCICR Test Number Dilution preparationinfo mation: Comments:

KCI CHM number: CM ItLL Stock preparation: 50 g KCVL: Dissolve 50 g KCI in I L Deionized water Dilution prep (mg/L) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 IS Diluent volume (mL) 994 991 98s 985 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organisminformation: Test information:

Organism age: Zl.2c ro 7 L.I5 t m Randomizing template: jtpt Date and times organisms is.ot o04 14NW -ro -so Incubator number and were bom between: shelf location: ___

Organism source: A61& 6AnA A\-O'-oq Arlemia lot number: 6& l Transfer bowl information: pH = SU Temperature = C Total drying time:

)AE8 *

2. t) 0 Date /Time in: 11 l;a0 1320 Average transfer volume: 5 OveDateTimpeout: 11 13 Oven temperatur : I I'C, Dailyfeeding and renewal information:

Day Date Morning Afternoon Test Initiation, Control water Analyst feeding feeding time renewal, or batch used time termination time 1 1cn.qL -O8S 170) lq02 li-i.N 2 _______ o>oc isc 11L4 2X 1.oq-o4 S 3 aI-12.-o 09151 Szi I .4S k 4 II1-IR-64 CA63 15h I 140t 4na9I~

-0 60-LI5f (A4-1 i~o6 Im31-1+ d 7 1111Si; KK fN;.'. ,g7;'. l4°l1X.,_Y, Controlinformation: Acceptance criteria Summary of test endpoints:

% Mortality: 07p £20% 7-day LC5 0 foqq. I Average weight per initial larvae: O.&.p Jb0jf ¶.t, j NOEC Average weight per surviving larvae: 0.(009 2 0.25 mS arvae LOEC ;40 ChV IC25 5 o

Environmental Testing Solutions. Inc. Page 2 of S Environmental Testing Solutions. Inc. Page 2 ot5 Species: Pimephalespromelas PpKCICR Test Number: 49 Survival and GrowthData Day Control 300 mg KCUL 450 mg KCUL'

_A B C D E F G rH I J K L 0 /6 /0 /0 JO 10, 0 /6 0 1( ) (0 IC) iL

-'o 1. lo IO- 1 IC ICo,O IC oa)t 2___IC)_

3 / IC)

,0 IIOI0 /0 0 I10 10 J /0 10 IL-)0 10 1/010 to /O l10 10 ro i 10lo____ io 10 Jo 16'I 1 10 1o

_ IC)___ I o0 10t to .lo lo IC) ( IC). £0 103_ 0 lo0 /0 i _01010 10 (0 o 10 I) 0 tOIC) to 10 1t0 IC) CC)

J)

_______ (0 LQ (05 lI i1- 9 O lO 1O qj £ A - Pan weight (mg)

Tray color code::n Analyst: _ CJ EL ;e '4 0 5 B - Pan + Larvae weight (mg) An Analyst: \ e, Larvae weight (mg) A- B S AD Lw Weight per Initial number of larvae (mg) -0

- C / Initial number ot larvae 4 t-v _.

%D O'I

'~D O* f0r of* 4 4,P Average Percent weight per reduction Initial from control 0. 609 0.57 .51 0*

-, 0%-

number of (%)

larvae (mg)

Comment codes: c = clear, d - dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed: 4_

Comments:

fEnvironmental 1'esting Solutions, Inc. Page 3 ot.)

k..nvironmental Testing Solutions, Inc. k'age 4 or, Species: Pimephatespromelas PpKCICR Test-Number: A19 I I I I

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Calculations and data reviewed: _

Comments:

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02.013, Method 1000.0)

Species: Plmppholespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test usben PpXCXCR 39 (49 Pt 351Depot St)

Test daste. Isveb 92 1004 Revelded byt C -e* Rp.k3.t d_n- -tWL A-?U."4(.) WIl 5* he Li WeIS1. c0 1n M _ Un. .4 2.- rutO".*_

t,_I2)

KC1 -A

3 _.2lt l - # t) 2I t.l t) WO3 (

A 10 l0 14.95 20.31 5.38 0.53O 0.553 castre! a 20 to 14.92 21.11 6.26 0.626 0.609 738 06126 100.0 0A09 I Not applecabfr C 20o 0 14.553 20.97 642 0 642 0.w2 D 20 )0 14.31 21.10 6.29 0.629 0629 6 10 10 25.03 20.76 5.73 0.573 0.573 P t0 20 t24.3 20.56 6.05 0605 0.531 37. 0605 73 023 2 I4.5

=G= to 9 15.23 23.12 3.58 0.398317 0.353 75 G1 13I.

I2 20 I20 15.19 20.66 S.47 0.547 0.547 10 I0 14.73 20.33 5.60 0.560 0.560 3 1 I0 t0 14.72 19.92 5.20 0.520 0.533 4.8 0.520 97.5 0324 4.8 34.0 K 10 9 14.70 19.74 5.04 0.560 0.504 L 20 tO 14.85 19.96 5.1 I 0.511 0.511 .

hi 20 a 14.72 19.15 4.43 0.554 0.443 Go_ O 10 S 14.98 18.69 3.71 0.464 0.512 7.6 0432 30.0 OAtS 7.6 32.7 O 20 S 24.92 19.217 4.25 0.531 0.425 P t0 8 14.42 13.42 400 0500 0.400 Q 0 3 4.63 16.20 1.47 0490 0.147 75 R 20 4 14.63 16.25 1.62 0405 00^6 S.0 0.172 2.4 71.

S 20 4 26.92 29.27 2.36 0.590 0.236 T 20 3 14.80 26.22 1.42 0.473 0.142 U 20 I 15.22 16.04 0.32 0320 0032 V 0 0 000 00 00 0 0.326 13.1 0.000 15.0 0.e27 93.5 79.2 04.70 17.51 2.81 32 0.937 0.222

_ __ __ _ _ _ X I20 J 2 3_4.45 25.39 1.44 _ _ _ _ 0.721__

_ _ _ _ _ _ _ .44 _ _ _ _ _ _

Dzometts MSD allets 3.309 MSD - Minimun Siznificaut Diiferenee PMSD: 13.0 PMSD - PerntmMiimWIn Sitnicunt DiTrrernce PMSD is&mearne eftest Precision. The PMSD isthe minnum perct ditfrrence bnwm the control and tstent thatcan be deciued itily ignifiat in awhole efluent todtylest. On avergre. asignificant diffrence occaurrot Envwvomntal Tefllig Solutions, Inc. chronic touxcy tests when atoxicurd veduces Iff phar growth by 15.6%ftloothe control (determined through reference toxicant Testht)

LOwePMSD botnd determined byUSEPA (10th rntile) - 9.4%.

UpperP S bounddetermined by USEPA (9th percntit) -35%

The lower andupper boumds were calculated by theUSEPA sing 205 tests conducted from 19lkborstoets for Pimephales growth in chroninc relrice toxicant testl USEPA. 2000. Und ndng and Accuhtig bMethod Viablityin Whole EtfluentToxidctyApplictions UndertheN lonal Pollutnt Dic eElinination Horm EPAA-33-R.)0-00.US Envinment Prtection Agency.CinnadOIL

,goptstrs obasedfemo Aquatic XboSvoreu. I[c. II,"4

Environmental Testing Solutions, Inc.

Statistical Analyses 1rval Flsb Crew* La4 Servival Tw;7 D7 ySan;v SRtuDate: 11191204 Tea lD, FPKCltR Saropl 1D, REP-RefToxkat End Date I In6i2004 Lab m. M13flf.TUdikASol SompleTpe: DMR-Dbcih rigReport Sample Date: Protoco FWCK2-BPA-t21I-R42-03 Test Speces: PP.? bales ptcnsla Commentb Coaeng/L 1 2 3 4 D-Control 1.0000 1.0000 1.00 I.Q000 300 1.0000 2.O. OX0O 1.0000 450 1.0000 1.000 0.9000 1.0000 600 0.1000 0.1000 0.IQ0 0.1Q00 750 03000 0.4000 0.4000 03000 900 0.1000 Q100 0.3000 0=20 Tnasfonm: Armat Squsr Rdot Rank I-Tsled Number Totl Canst-eL ll NfMe N.MrNl Mless Min MIX CV% N Sam iteaIl Res Nasber D-Conlor 1.0000 2.0000 1A120 14120 14120 0.000 4 0 40 300 0.9150 OS750 13713 1.2490 1.4120 5.942 4 16.00 10.00 I 40 JS0 0.9750 09750 1371 1.2490 1.4120 5.942 4 6.00 10.00 1 40

600 0.1000 0.1000 1.1071 11071 1.1071 0.000 4 I0.00 10.00 1 40

750 03500 0.3500 0.6322 0.5796 0.6U47 9.597 4 10.00 10.00 26 40

900 0.1500 0.1500 03110 0.Q5l1 0.5796 47.729 4 10.00 10.00 34 40 Awi2l2iryTests Statistic Critcl Skew Kvrt Sbapko.WiksTeat bdiates noonudkbtrution (p> 0.01) Q.S955315335 0.1Q4 -01410811 2596177193 E!mM2 ofwurjnre canot be confimed 1Hpolesf&Test (n-tll,0.05) NOEC LOEC QV STU M

Steels any.OwR nk Test 450 600 519.6152423 Testments vs D.Control MaximaumLUketh..d.?r.bit Parsteter V.5.. SE 95%F2declalLhwilts Control ChI.Sq Oleiel PhValue Me S14ma Iter Slope 3393696931 3366205654 *23140717 19.11146556 0 25.42745675 7.114773 13E-05 2f44518145 0.119066019 4 Intercept *1.1190.451 9.5074232 *49.2153196 11.504S9799 SSCR 1.0 Poent Frbbll mg/L 956%FIduetIl lt 0.9I ECOI 2.67 369.42716 4 EC05 3355 4453196461 0.e ECIO 3.711 491.9588902 0.7 EC15 3.964 526.155457 ZC20 4.15S 555.02Q1233o EC25 4326 521.0443574 EC4O 4.747 652.154076 EC5000 699.065461 04 EC60 5.253 7493473942 0.:

EC75 5.674 S41.0600443 0 ECSo 5.U42S10.4946016 0.2 ECIS 6.036 921.8000412 0.1 EC90 6.2S2 993.3621247 0.0 EC95 6.645 109739S65 * ¶0 100 IDoo 1c100 EC99 7.325 1322.340041 Siieanf t hetergenelty detected (p - 1.26E.05) nL Doss.RaspossePlot 019 0.7

! 0.5!

0.4 P.

0.3 :

0.2 0.1 I

Orgaru~sms obtained fiam A juafkc RibSystans. Inr-

Environmental Testing Solutions, Inc.

Statistical Analyses Lam] Fla Crowth sad Srvval Test-7 Div GCrnw Star Date: I I N2004 Tat lD PpKCQCR Sample D. REF-RefTobikt EndDaste: 111612004 Lab ID. E7M3 ir.TeTutsig5oL SplerTypc DM?.-Disd-pgMnbrzktiRtpwt Samnple Dte: fotOCt FWC8R-EPA.821-R.2=013 Test Specs.: PP-Pimcpb&W ptociks Cormnent:

C*%c-a/VL 1 2 3 4 D-o lfroI 0.S380 0.6260 0.6420 0.6290 300 0.5Mo M6C50 03580 0e470 450 0.5600 0.5200 0.040 0.5110 600 a4430 03710 04250 0.4000 750 0.1470 0.1620 0.260 0.1420 900 0.0520 0.oooo 0.21o 0.14O Tnstormr std I-Tte Isoa Cone-mm. Meas N-Mfta Mens Min Max CV% N t-Stat CrItkal MSD Mess N-Mtsa D-Cotrol 0.6088 1.ooo0 0608 05330 0.6420 7.832 4 0.6088 1.0000 300 0.5208 0.8554 05206 03510 0.6050 21327 4 1.746 2.180 0. o06 0.5223 0.2579 450 03528 0.8604 03238 0.040 05seo 4.781 4 1.687 2180 0.1098 0.5223 0.8579 600 0.409s 0.6731 0.4098 03710 OU." 7.633 4 0.4098 0.6731 750 0.1718 0.2t21 0.1718 0.1420 02.60 25.425 4 71 0Q2S21 900 0.126 - 0.M2082 0.12SI O.OX00 0.2S10 93.527 4

126S 0.2082 AwsrdaryTeot Stitatic Crtdcal Skew Kurt SbApro-Wik Teestkidictesnosma dntre lk(p>0.01) O.1173097 0.805 -1.355 19 3.162416858 WW9sTest kidicats equal v es (-007) M524760 5 9.2103405 I1pthels Test(1-ftlD 0.e5) NorC LOEC CbV TU MSDw MSDp MSB MSE F.Prob df DmnetrsTest 450 >450 0.10961417 0.180451116 0.009985333 0.00507125 0.195662111 2.9 Seatimets vs D-Cntrol LUaearlstrelati. (200 t Resenspla)

Polnt ig/.L SD 95% CL(Exp) Skew IC05o 105.56 93.66 42.68 529.28 1.0619 IClo- 211.13 108.23 8536 614.46 0.4423 ICIS 456.42 I08.32 44.21 532.87 .0.4i92 1.0 IC20 497.00 77.10 125.81 57638 2.0308 0.9 IC25 53732 4ZO2 439.61 624.40 .3.2045 IC40 621.05 10.95 596.86 663.02 0.1345 0.8 ICSo 666.48

kdates IC estiaute II=

9.76 1ht kyrut c=ctr&U6 640.69 699.57 02168 I0.6 e.7 0.5 4:0.4 03 02

.1 0.0 0 200 400 600 800 1000 eU.o..tsu ....e..... Dose mg/I.

0.8 0.6 . . . . . . . . . . .i. . . . .. . . . . . 1-tan. 0.05 1lVI of lgorennce 0.4

~03I, o O.t 0.2 0.1-eq.

OrganLmuobtanedfrom Aquneic BNoSystfem, Inc 1-09044

AZjjj W11 JA1AAhCM41 I t:SC1112 601UtIOflS. InlC. Page 4 of 5 Species: Pimephalespromelas PpKCICR Test Number: q Daily Chemistry:

Day

_0 1 1 2 Concentration Parameter I CONTROL pH (S.U.) -O-o 1.%9 9 .DZ 11.CS DO() 1.1 i 1.5 1. I Conductivity (pmhos/cm) z -

Alkalinity 62 (mg CaCO 3 /L)

Hardness (mg CaCOAI) o Temperature - 2q. .2q. Li pH (S.U.) 2.' 1 qZ 1. 1 .O5 0'.o4 1-88 DO (mgL I, 1. 3#1 -L 0%

300 mg KCC/L Conductivity (gmhos/cm) U)s a 3 ! 8 Temperature z.S pH (S.U.) 1 1.q 5 S.e5 1.o1 1Q DO (m) 8.0 1.17 o.

450 mg KClL Conductivity _ _

(.irnhos/cm) 1 200 I Z ZC OI F Temperature 24l 2'Z3 2'L9 Z4.' 5 S.S p2H (S.U.) S. tO O3 8.5

'aC 18 9.O-1 1 DO (m__ _) _O____

_ _ _ 18 600 mg KCILL Conductivity (pmhos/cm) ILiF53 ALb _

Temperature

(°C) X$. I ;4 .S 2.9 24.q 2I -a pH (S.U.) g.lO 1.o'I 07.q0 8-o DO (mglL) iWq 7O 1. 1.1 750 mg KCIIL Conductivity (Tmhos/cm) pI130 liqo Temperature 2q.9 ;2q. 35 . 2t1.3 i. o t.3 pR (S.U.) %.o)S I -L'15 I y.o9q 1.9I DO (mg/L) 8,D- . §.O 900 mg KCV/L Conductivity ZOO (gmhos/cm) .- ZOo l1EA Temperature (0C) 2zq. *S STOCK Conductivity I(gmhos/cm) Il _. I Initial Final Initlal I Final _

I Initial I Final

.rnvironrnental l estinLg Solutions. Inc. Page 5 of S

.i.nvIronmenca1 festin2 Solutions. Inc. Page 5 of S Species: Pimephalespromelas PpKCICR Test Number: AA.

Day Analyst e~J4 P'L8 Fe-R _ C- ofi ~

Concentration Parameter CONTROL pH (S.U.) .5 17.67 B.1Z 7.I6S .oS 1.l 1.

Conductivity nt1° .Ol1 .

(iinihos/cm) 3SU.P 301 Alkalinity Hardness (mg CaCOVL)

Temperature -z, I IN q .9 L'At Z'4A 'tS *Z5.3 25.

pH (S.U.) 1__g15. _ _ _ _

DO (mg/L) 1.. 1 300 mg KCI/L Conductivity (limho /m) S2.51 Caz1 Temperature ZS.O 0 i. 7.oz 2, 2S7.0 Wl - 2.7 s pH (S.U.) BAS Z t ^ .3 54 i.6 0; 121 5. lS DO (mgC) 1.5 '7.71 7.7 2S*I 8.D 2.41 .,9 1 450 mg KCI/L Conductivity ._.

(Vmhos/cm) 10 70 _,

Temperature -Zs. ,q. S *1 2q.i 2S.O L. 25.O ZS pH (S.U.) 7Ll0s 600 mg KCI/L, DO (mg/L) 15 .Z .000 s iO 1e Conductivity o2.Z (pmnhos/cm) 4 1q30 14Z Temperature ; 2'I1 Z5,O 2S.O I.9 Z5.2 25.

(0c) 2. 5. s-7 IS pH (S.U.) T- 1 7 6 8. I9 .6 B0, IL V51 7.q 3

-\

DO (mg1L) 1I. 7 7.1 . . 7.9 750 mg KCIlL Conductivity l680 __1_0 Temperature 2qui _..

2sZz _ Z Zs. 25 s

________ ('C) 4q 2.1 PH (S.U.) 4 - 4 - F -------- II 1.67 I -..------ .41-1.1%

- I-9.13 4

- a DO (mg/LI 7.Z It

_ * - F bn,.

900 mg KCiL Conductivity

(,umhoslcm) I 1q___O 1950 IPQ{

- - -. -0 _ _1. I, L ,L I

Temperature (fC) 2;.1 q I 2q. 8 '2q. e zq .7 r25.3 STOCK Conductivity I (gmhos/cm) Ig (o1OO 78boo 7970b Initial I F Initial Final Initial I Final I Initial Final

/

Environmental Testing Solutions, Inc.

Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.20 I I I I I I I I I I I I I I . I I I I I I 1.15 State and USEPA Mandated ControlLimits Limits Set According to +/-2 StandardDeviations 1.10 ............ ......................... ...............................................

1.05 1.00 0.95 U

'0 1.5 1.4 Laboratory Warning and ControlLimits 1.3 Limits Set According to 1H/h and 2 5thPercentileCVs 1.2 1.1 1.0 0.9 ........................ ............................... ..................................................................................................

0.8 Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.

- - Central Tendency (mean IC25 )

-Warning Limits (mean IC25 4 SA_o)

. Control Limits (mean IC25 +/- SA or 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Sodium Chloride Chronic Reference Toxicant Control Chart for Cerlodaphniadubla using Moderately Hard Synthetic Water State and USEPA Labosatory Labontory USEPA USEPA Test nunter Test date 7-day IC2, CT S Certd.lLalits S5.,, Waning Lnits SA2 ControlLmits Sms Waring Limits SAM Csntrel Lhits CV (gtLNaCl) (gILN&CI) CT-2S CT+2S CT-SA.10 CT+S~U CT-SAJ9 CT+SA2s CT-SA.7 CT+SAJI CTr- SAM CT+SA.

1 05-6-03 1.05 2 06-4-3 1.07 1.06 0.01 1.03 1.09 0.0 0.97 1.14' 0.18 0.88 1.24 0.48 0.58 1.53 0.66 OAO 1.71 0.01 3 07-08-03 1.03 1.05 0.02 1.01 1.09 0.08 0.96 1.13 0.18 0.87 1.23 0.47 0.58 1.52 0.65 0.40 1.70 0.02 4 08-05-03 1.04 1.05 0.02 1.01 1.08 0.08 0.96 1.13 0.18 0.87 1.22 0.47 0.57 1.52 0.65 0.40 1.69 0.02 5 09-10-03 1.05 1.05 0.02 1.02 1.08 0.0O 0.96 1.13 0.18 0.87 1.22 0.47 0.58 1.52 0.65 0.40 1.69 0.01 6 10-07403 1.02 1.04 0.02 1.01 1.03 0.0 0.96 1.13 0.18 0.86 1.22 0.47 0.57 1.51 0.65 OAO 1.69 0.02 7 11.04-03 1.08 1.05 0.02 1.00 1.09 0.08 0.96 1.13 0.18 0.87 1.23 0.47 0.58 1.52 0.65 0.40 1.70 0.02 8 12.04-03 1.08 1.05 0.02 1.01 1.10 0.08 0.97 1.14 0.18 0.87 1.23 0.47 0.58 1.52 0.65 0.40 1.70 0.02 9 01406404 1.06 1.05 0.02 1.01 1.09 0.08 0.97 1.14 0.18 0.87 1.23 0.47 0.58 1.53 0.65 0.40 1.70 0.02 10 02-03404 1.06 1.05 0.02 1.01 1.09 0.08 0.97 1.14 0.18 0.87 1.23 0.47 0.58 1.53 0.65 0.40 1.71 0.02 11 03403-04 1.07 1.06 0.02 1.01 1.10 0.08 0.97 1.14 0.18 0.88 1.23 0.47 0.58 1.53 0.65 0.40 1.71 0.02 12 0446O04 1.07 1.06 0.02 1.02 1.10 0.08 0.97 1.14 0.13 0.88 1.24 0.48 0.58 1.53 0.65 0.40 1.71 0.02 13 0504404 1.07 1.06 0.02 1.02 1.10 0.08 0.97 1.14 0.13 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.02 14 06-0S4 1.06 1.06 0.02 1.02 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.02 15 07407404 1.06 1.06 0.02 1.02 1.09 0.08 0.97 1.14 0.18 0.83 1.24 OAS 0.58 1.53 0.66 0.40 1.71 0.02 16 08-10404 1.06 1.06 0.02 1.02 1.09 0.08 0.97 1.14 0.18 0.88 124 0.4 0.58 1.53 0.66 0.40 1.71 0.02 17 09-1404 1.05 1.06 0.02 1.02 1.09 - 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.02 18 09-14-04 1.07 1.06 0.02 1.02 1.09 0.08 0.97 1.14 0.18 0.88 1.24 OAS. 0.58 1.53 0.66 0.40 1.71 0.02 19 10-05-04 1.07 1.06 0.02 1.03 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.58 1.53 0.66 0.40 1.71 0.02 20 11402404 1.06 1.06 0.02 1.03 1.09 0.08 0.97 1.14 0.18 0.88 1.24 0.48 0.53 1.53 0.66 0.40 1.71 0.02 Note: 7-d IC,, day 25% inhnbition concentration. An estimation of td concentration ofsodium ehloride that vould cause a 25% reduction in Ceriodaphnfa reproduction iot the test population.

CT - Central tendency (mean lC2,).

9 - Standard deviation othe IC 3 vaIuesL Laboratory Control and Waning Limits Labontory contl and warning limits re established using th standrd deviation ofth C valws corrsponding to tho 10th and 25th percentile CV Thes ranges are 1mrr sthingent than the control and wrning limits recomnmended by USEPA for the test method and endpoint.

SAIS - Standard deviation corresonding to the 10t percentile CV. (SAI e-0.08)

SA2s - Standard deviation eoresponding to the 25" percenble CV. (SA.25 - 0.17)

USEPA Centrol and Waning Limits S, 7s- Standard deviation corresponding to the 75* percentile CV. (SAl, - 0.45)

SA. - Standard deviation corresponding to tho 90" percentile CV. (SAe - 0.62) cV - Coefficient of aristion orthe iC., values USEPA. 2000. Undetanding nd Accounting for Method Vriability in Whole Effluent Toxicity Applications Under the NStional Pollutant Discharge Elimination Prognrn. ElPA-833-R4004003. US Environnental Protection Agency, Cincinnt. o.

IIf.044

Environmental Testing Solutions, Inc.

Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.8 I I I I I I I I I I I ~ I I I r I r r I 1.6 I 1.4 I 1-%

Uel z 1.2 _ . _... ... _ ... _ .. _ .. _., .......... _..._..... .

1.0

'-4 in U

0.8 I 0.6 - - _________________________------_

0.4 F I II I I I I I I I I I I 0.2

'I, N"' A'.* Test date Test date 7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.

- - Central Tendency (mean IC2 5 )

Laboratory Warning Limits (mean IC25 +/- SAI10, SAJO = 0.08)

Laboratory . Control Limits (mean IC2 + SA25, 8 A25= 0.17)

-- USEPA Warning Limits (mean IC25 +/- SA.75, SA .75 = 0.45)

USEPA Control Limits (mean IC25 + SA.90 SA90= 0.62)

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Tes Test date Control Control Mean CT CV CT MSD PMSD CT number Survival Reproduction for Control Mean for Control (3/4) (offispingivrmale) Reproduction (M) Reproduction (3/4/.) for PMSD Ch)

(offspring/female) CV (%/.)

1 05406403 100 27.6 10.8 3.2 11.5 2 06-04-03 100 25.9 26.8 5.9 8.4 2.6 10.1 10.8 3 07-08.03 100 29.0 27.5 11.6 9.4 3.2 10.9 10.8 4 08-05-03 100 33.3 29.0 6.6 8.7 4.7 14.1 11.6 5 09-10-03 100 29.3 29.0 4.3 7.8 3.1 10.7 11.5 6 10-07-03 100 33.4 29.8 8.0 7.9 3.2 9.5 11.1 7 11-04-03 100 31.0 29.9 7.3 7.8 2.6 8.3 10.7 8 12-04-03 100 30.4 30.0 9.7 8.0 3.6 11.8 10.8 9 01-06-04 100 30.6 30.1 4.4 7.6 3.5 11.5 10.9 10 02-03-04 100 30.7 30.1 7.2 7.6 2.7 8.8 10.7 I11 03-03-04 100 29.0 30.0 7.8 7.6 3.0 10.3 10.7 12 04-06-04 100 28.1 29.9 6.2 7.5 2.8 10.0 10.6 13 05404-04 100 32.5 30.1 6.4 7.4 3.3 10.1 10.6 14 06-08-04 100 32.9 30.3 6.8 7.4 3.0 9.0 10.5 07.07-04 100 33.3 30.5 6.3 7.3 2.6 7.8 10.3 16 08-10-04 100 27.4 30.3 5.8 7.2 2.3 8.6 10.2 17 09-14-04 100 28.7 30.2 4.9 7.1 2.4 8.2 10.1 1B 09-14-04 100 28.8 30.1 7.5 7.1 3.0 10.5 10.1 19 10-05-04 100 29.6 30.1 8.2 7.1 2.8 9.6 10.1, 20 11-02-04 100 30.5 30.1 8.5 7.2 2.7 8.8 10.0 Note: CV- Coefficientofvariition for control reprodaction.

On average, the CV for control reproduction is 7.2% in Environmcnta] Testing Solutions, Inc. Ceriodapmnid chronic toxicity tests.

LowerCVbound deterined byUSEPA (10' percentile) -8.9%.

UpperCVbound determinedbyUSEPA(90'pcrcentile) - 42%

MSD - Minimum Significant Diffirence PMSD - Percent Minimum Significant Difference PISD bis measure oftestprecision. The PMSD isbthe inimum percentdifferencebetween the control and trematentthat can be decared statstically significant in swhole efmnent toxicity tesL On avcnge, a sinificant diffcrence occurs for Environmental Testing Solutions,Inc. chronic toxicity tests when a toxicant reduces Ceriodaphniareproductionby 10.0%

from the control.

Lower PMSD bound determined by USEPA (1 0' percentile) - 11%

Upper PMSD bound determined by USEPA (90*percentile) - 37/.

CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD)

The lower and upperbounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cenodaphnia reproduction in chronic reference toxicant tests.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the Nabional Pollutant Discharge ElirinationPrognra EPA-833.R-0003. US Environmental Protection Agency, Cincinnati, OH.

11-02-04

Environmental Testing Solutions, Inc.

Ceriodaphniadubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests 40 35 n 30 .............................

CJ r 25 9_ ..... \.

'r a 20 -..-.._.~ ~.._..._

15 Minimum Acceptance Criteria (15.0 offspring per surviving female)

. I I I I I I I I I I I I I I I I I I I I .

-I I I I I I I I I I I - I I I 40

.- c North Carolina Acceptance Limit (< 40.0%)

30 C o4 Kentucky Acceptance Limit (< 30.0%/O)

-0 o -

20

= 0 CJZ 10 UZ CI J 6o 1S6o34n30hr9EwNKCN0wN#Ws'3.voN0#030#olStS0#o, tk'.k Ak tk % '

S~O jkO9-oovX Jo%,01, ANo Test date

- Control Reproduction or Coefficient of Variation (CV)

- - Central Tendency (mean Control Reproduction or CV)

.. Control Limits (mean Control Reproduction or CV 4 2 Standard Deviations)

Environmental Testing Solutions, Inc.

Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 45 40 35 0 30 I. . I. .. I.I. .* . .. I ..I.I. . . . :

= 25 pI 20 15 10 I I =I pm _ II _= = M_ id 5

0 A. & , 1 t xaI k INS- tv .rA °8 %9`\° N!", NN~ovtNO oll %1 ro o qA°9 '° oF0 °<oS° tv \° ac'49 9'tq- 0°w" Test date

-- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

- - Central Tendency (mean PMSD)

.-- Lower and Upper PMSD Bounds Lower PMSD Bound ( 1&thpercentile) = 11%, Upper PMSD Bound (9 0th percentile) = 37%

(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)

Environmental Testing Solutions, Inc. Page I of 6

p Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphniadubia CdNaCLCR#: 2 Dilution preparationinformation: Comments:

NaCI CHM number: CIA 1 Stock preparation: 100 g NaCIl (dissolve 50 g NaCI in 500 ml deionized water)

Dilution prep (mg/L) 600 800 1000 l 1200 1400 Stock volumc (mL) 9 12 15 18 21 Diluent volume (ML) 1491 1488 1485 1482 1479 Total volume (mL) 1500 .1500 1500 1500 1500 Test organism information: Test information:

Organism age: * < 24-hours old Randomizing template: POAte Date and times organisms ll O 1'b1 1l2z_ Incubator number and were born between: shelf location:Z6 Organism source: I 0 -Z6,-A A-F YCTbatch: toS Transfer bowl information: pH =*1. SU Temperature =2YLPC Selenastrum batch-4e

. rj-1S.Oq Daily renewal information:

Day Date Test Initiation, Control water batch Analyst renewal, or used terminatIon time o 1I-c2.-o'l 1z OIlat -c 2 II-aYZ-0 173*5 ___-__ _-O__

4 IIOtt I'St 2-6 6 _ _-__ _ _ I c sc i-7 11-o0% Otti I~I5 Control information: Acceptance criteria Summary of test endpoints:

% of Male Adults: 07e S 20% 7-day LC50 7>l4cct

% Adults having 3d Broods: loy k 80% NOEC 800

% Mortality: O7: s20% LOEC loco Mean Offsprinr/Female: I offspring/female 25.0 ChV 894.

%CV: <40.0% IC25 1s55.3

-]Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphniadubia CdNaCLCR X: 2q CONTROL Survival and Reproduction Data F

Replicate number vs Day 1 2 3 4 5 6 7 8 9 I 10 o I I I

I Young produced T T 0 Adult mortality l 2 Young produced l Adult mortality J L .

1 1

or lLl>l

<lL 3 l Youngproduced Q O 0 O O O Oc)

Adult mortality l \ -- L- \.-

4 Y oung produced

. Adult

. . mortallty

_ __ _ __ _ l l4 -AI ;

\.-Ilt \_

1 L-ILA .

_ L-1 LA I l >l S Young produced to lI L) II lto I i9 C i lb to .

.Adultlmonality L- Ll I L L -

6 Youngproduced J0 .O Tro r 0 O j Adult mortality L -lL- LL- l- = l-7 Youngproduced l16 i I- d 15 l &1 tA R I Ilo 27 \ l 29 l '9 2 3. l 3c

{ I Total young produced j

31 at Final Adult Mfortality _. %_ . _ ' L- %' -%.

Xfor3 ~Broods l~. >cClz Cl;Clb Cl Cl Concentration:

%Mortality: I 07.

Mean Offspringffemale: I2.5 1 600 mg NaCI/L Survival and ReproductionData Day 1

Young produced I

l 1 l 2

j 3

~

0 4

l O

!~RepaIte number 5l 6 7 8 9 1 10 Adult mortality - TL L ' . t- 1 2 Young produced l l T r -

'Adult mortality U1CT

?I

C 33 Young produced T o 7 I I Adultmortality 1 1 =

s,_ _ __ _ I lc l t.11 t1 Adult mortality . L I

_

5 Young produced U C)101' kfB Adul mort C- = - _ -: = =_

6 ung produced 1 j.a 1IO { . J I ! Adult mortality L L- I L L I L 7 l Youngproduced III l IL l l 1(4 II LA l i 5 l5 ito ItI"l Total youngproduced l 32- l Z ri 3Z l3 2 r l 9 3c 32. l 2 l Final Adult M ortality l lL l l ? l l lll

L I I Concentration:

%Mortality: Cf7 I Mean Offspring/Female: I 1

%Reduction from Control:

PEnvironmental Testing Solutions, Inc. Page 3 of 6 r Species: Ceriodaphniadubia CdNaCLCR #: Z1 s -'.. 800 me NaCI/L Survival andReproduction Data Replicate number 4

y Da Young produced 1 2 OTf 3 5 6

+/- 7 9 9 110 JI Adult mortality Tj . L U 1_

2 Young produced Adult mortality r)L- Q10 L- 0%-- IL 0)

_ .. .I I cf CL- _ L _ - I_

3 I F

Young produced Adult mortality C) C)L 1 X OI Q

(

TO 4 Young produced O O _.

Adult mortality j L U I \ . t U 1 '

5 Young produced l V2- L O .JLIk 0 j O O 153 _

l Adultmortality .l l L- L_1 L L- l tlU L L-6 Young produced o 0l0 lZ C O 0 IL 9 ll Z_ °

- ~Adult mortalityj L-. L. I -- U.

L- L- L- L- ..

7 Youngproduced l 1ho 1, ILI It is II lS t3 Total young produced I T Iz- 3 : 3 1 3 .

Final Adult Mortality l l ?I ... I l* l*l l-* l1 iF** l Concentration:

l % Mortality:

Mean Offspring/Female: I l

% Reduction from Control: -1 .o%

1000 mn NaCI/L Survivaland Reproduction Data Dav 1 lYoung produced 11 1 2 3

_4

-Replicate 5

number L)la 6 7 C )lC {O 8

lO 9

lO 10 I

Adult mortality l 1, l U Xll U U U 2 Young produced C O 3 Young produced 07 IoI-aIOI 1c I° C I Adult mortality 4 Young produced l _I I L1 3 W Adult mortality J U l l l I

t l l ' L-.

5 Young produced J .. LS IL 01k Q 0i. 0..'iI. I~

Adult mortality I LU L- L 6 Young D produced 10 - -- - -C - I SpLT f!tzoOb j Adult mortality 1 i L - I 7 Youngproduced ij l Jo l 16 13 10 I's Totalyoungproduced l-2 lRVo l30 l271 l ZI 28 2o Z VI Final Adult Mortality - - . T l 7 l L ll I%

Concentration:

Mortality:

Mean Offspring/Female:

07.

I 71.(O

% Reduction from Control: l

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphniadubia CdNaCLCR #: 2VI 1200 mg NaCIL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 2

I oug roucd-Young produced Adult mortality t . T O 0 L.

a

?

_.O 0O r -

C)

-O 3 Young produced od O 0 0 O OI(1 I O Ioun Adult mortality Youngproduced j =

O C) L a Adult mortality J . L . 1 s Adult mortality.

7 Young produced a3 22 I '3 l -

To pro Ytunoung jAdult duced le] l l _t -

mortality l llI-l l j i U U.

61l Young produced lLO lI 3 _ )l 01 zG~0lOlC 010 l Adult mortality l~

-*01 Jl~ U. l ? \. u l l

U. '-

Total young produced l 9 r ,-T ra J .az l Jo

.l ,

Final Adult Mortality l l *t ~ U.L 1 .. .. L ...lL.

Concentration:

% Mortality: i107%

Mean Offspring/Female: I i. ( l

% Reduction from Control: (,oS. I%

1400 mg NaCI/L Survival andReproduction Data

[ - Replicate number -

Day 1112 3 141516 7 1819 10

- T Adult mortality -

U G

U

( -&-

6 UI U. LCS 2

2 Young produced

. Adult mortality

-Young produced Adult mortality

[.

L t l L-l, I

l O I JL-l L-llLl 3 Young producedl O- l O C 4 Young produced Adult mortality 0 ____ U. U_ Ll 0 0 LlU.- a -ZU &U LU 6 You poue __

Adult mortality aL l e -< I+ + tr II ! Youngproduced Adultmortalty !

l 1Jl! T1 U. L !- U.! I L L jLgt

__a Tt6 Young produced IL [la AdultAmortality 1., ouneo.duce.

[l Ut.

C)

K T,--

O l 0 l 1

Final Adult Mo0rtality 1C 1 v >l > 4'll > _

Concentration:

% Mortality: l 0.

Mean Offspring/Female: '4.2 2

% Reduction from Control: 8B6.2%

Environmental Testing Solutions, Inc.

Verification of Ceriodaphnia Reproduction Totals Control 1000 mg NaCl/L Day Replicate number Total l Da l Re 1lcate number Total Day 22 3 4 S 6 7 R 9 i 10Tl D 1 2 l 3 l 4 5 l 6 l 7 5' l l To

- O -O O- O - O - - I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 _ 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 _0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 4 4 5 4 4 4 4 4 5 4 42 4 3 4 4 4 4 4 4 3 4 4 38 5 10 10 10 1_ 10 12 11 9 13 10 106 S 11 8 10 10 0 1 0 9 12 8 79 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 7 0 12 0 0 2 21 7 17 16 14 161513 1418 6 157 7 144 14 16 16 13 12 14 10 15 138 Total 31 31 28 31 29 29 33 27 36 30 305 Total 28 26 28 30m 27 28 28 26 l_26 l 29 l 26 600 mg NaCI/L 1200 mg NaCIiL Repllcate number l y lRe plIcalte number I o I Day t 2 3 4 5 6 7 8I 9 10 Total DaY 1 2 3 4 5 61 7 9 1 Totl I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 4 4 4 4 4 3 S 40 4 2 3 4 4 3 4 2 4 2 3 31 5 1 10 9 10 12 _ 00 1 13 10 97 5 4 0° 4 3 3 0 7 5 7 3 6 0 0 0 0 0 0 10 0 0 0 10 6 0 3 0 0 0 0 3 0 0 0 6 7 17 16 14 1 17 415 15 16 14 156 7 3 3 2 0 6 3 0 8 4 6 3 Total 32 30 27 32 33 29 29 30 32 29 303 Total 9 9 7 l8 12 10 5 19 11 16 106 800 mg NaCI/L 1400 mg NaCl/L Day Replicate number Total Day Re plcate number Tota a 1l 2 3 1 4 5 6 7 8 9 10 l o 1 2 3 4 5 6 7 8 X To tl 1 0 0 0 0 0 0 0 0 0 0 0 1 .0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 4 4 5 4 4 4 43 4 3 3 1 0 3 2 0 2 1 2 17 5 12 10 ° 0 I 0 0 0 13 11 68 5 0 0 4 0 0 0 0 0 2 1 7 6 0 0 12 0 0 1t 9 12 0 0 44 6 1 4 0 0 0 1 0 0 0 0 6 7 31 13 14 19 16 15 l9 317 15 13 153 7 2 3 0 3 1 1 0 2 0 0 12 Total 33 27 30 34 31 31 29 F33 32 8 30 Total 6 110 I5 3 4 4 0 4 13 13 42

Environmental Testing Solutions, Inc.

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)

Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Tnt number: CdNaCICR #5S (R29 at 351 Depot St.)

Test datn: November 2-9, 2004 Reverwed by: & )l -

Concentration _ Replicate number Survival Average reproduction coefficket of rmrst redecdo. bo.

(mg/LNaCl) 1 2 3 4 5 6 7 - 9 10 (%) (ofrspring/remale) ,. . / (%)

Centrol 31 31 28 31 29 29 33 27 36 30 100 30.5 S5 Not applicable 600 32 30 27 32 33 29 29 30 32 29 100 303 6.2 0.7 800 33 27 30 34 31 31 29 33 32 28 100 30.8 7.5 *1.0 1000 28 26 28 30 27 28 28 26 26 29 100 27.6 4.9 9.5 1200 9 9 7 8 12 10 5 19 i1 16 90 10.6 39.6 65.2 1400 6 10 5 3 4 4 0 4 3 3 100 4.2 61.3 86&2 Dunnelt's MSD value: 2.693 MSD - Minimum Significant Difference PMISD: 8.S PMSD - Percent Minimum Significant Difference PMSD Is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphniareproduction by 10.0% from the control.

Lower PMSD bound determined byUSEPA (10' percentile) -11%.

Upper PMSD bound determined byUSEPA (90 percentile) - 37%.

The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories ror Ceriodaphnia reproduction in chronic reference toxicant tests USEPA. 2000. UnderstandingandAccountingrorMethodVariabilityin WholeEmuentToxicityApplicationsUndertheNationalPollutantDischargeEliminationProgram. EPA-S33-R.O.003. USEnviromnentalProtection Agency, Cincinnati, OH.

Environmental Testing Solutions, Inc.

Statistical Analyses Cerfldapi.sla Servival &adRepredseuton Tuet-Reprodoetles StatDate: 1112004 Teet lD. CdNaCIR Sampke ID. REF-RafToxicant End Date: I J9/2004 Lab I: ETS-Pnnvr. Testing Sol Sample Type: NACL-Sodimm thbde Sample Dat Protocol CHP.ONIC-EPA-821-R-02.013 Tat Species: CD.Ca pbni dubia Comments:

Case-mgilL 1 2 .3 5 1 7 a 9 10 D-Control 31.000 31.000 2t.000 31.000 29.000 29.000 33.000 27.000 36.000 30.000 600 32.000 30.000 27.000 32.000 33.000 29.000 29.000 30.000 32.000 29.000 100 33.000 27.000 30.000 34.000 31.000 31000 29.000 33.000 32.000 22.000 1000 21.000 26.000 28.000 30.000 27.000 22.000 23 000 26.000 26.000 29.000 1200 9.000 9.000 7.000 2.000 12.00O 10.000 5.0DO 19.000 11.000 16.000 1400 6.000 10.000 53000 3.000 4.000 4.000 0.000 4.000 3.000 3.000 Tastsform: Ustrssfervad4 1-TIled l Xtaxle Coxe-eg/L Mesa N-Mess Mesa Min Mar CV% N t-Stat Critical MSD Mesa N-Mes D-Cocfrol 30.500 1.0000 30.50 27.000 36.000 2.501 10 30.333 1.0000 600 30.300 0.9934 30.300 27.000 33.000 6.233 10 0.170 2.2t7 2.693 30.533 1.0000 200 30.200 1.009t 30.t00 27.000 34.000 7.467 10 -0.255 2.217 2.693 30.533 1.00CO

1000 27.600 0.9049 27.600 26.000 30.000 4.t91 10 2.462 2.287 2.693 27.600 0.9039

1200 10.600 0.3473 10.600 5.000 19.000 39.572 10 16.295 2.217 2.693 10.600 0.2472

1400 4.200 0.1377 4.200 0.000 10.000 61.271 10 22.322 2.287 2.693 4.200 0.1376 AnxiliayTestm Statistle Critical Skew Kort Kolmogorov]DTest indicates ormaldisztrsop> 0.01) 0.9521032 1.035 0.78107399 1.6606005 Baietn Test idicates eua1 variances (p - 0.03) 12.2324052 15.0162722 KrAotheuil Test (1-tall. 2.25) NOEC LOEC Ct Y TV MSDs MSDp MSB MSE F-Prsb df Dunne Test 2OO 1000 t94.427191 2.69342413 0.08830901 1392.14667 6.93703704 1.4B-33 5.54 Trtatmrnt vs DiConrol UJsurlstterplatis (200 Resamples)

Poibt nmgL SD 95% CL Skew ICOS 904.090909 70321427 714.476471 942.65625 -3.2539 ICIDO 10OL41176 29.4310354 903.551627 1011.31664 -1.6769 ICIS 1019.37255 2.31342372 995.465193 1029.793 -1.3950 1.0 1C20 1037.33333 7.30725691 1012.79069 1049.32607 40.3208 02 IC25 1055.29412 7.37316969 1039.31161 1063 45042 .0.0324 IC40 1109.17647 9.79468145 1093.36587 1127.94776 0.4878 0.8 IC50 1145.09804 12.192936 1126.10741 1170.73316 0.6130 0.7 0.S c0.5 C ~0.

0.3 0.2 0.1 0.0

.0.1 0 500 1000 1100 Dloss, mgiL.

Date-Ratpense Plot 1i-tal, 0.05 level or signricrCe

'a 11.02-0.4

-. Environmental Testing Solutions, Inc. Page S of 6 "I.,

i Species: Ceriodaphniadubia CdNaCLCR. #: _201

Daily Chemtistry.

Day 0 1 2

_Analyst CAJ CY~d efw C431 eAJ Concentration Parameter '

pH (S.U.) . 1.4q- 19.C3 - 8.CA 7 .03s 17.qC S DO (mg/L) S T.. 6 9'.0 41.E 1 CONTROL Conductivity2S -

Alkalinity \

Hardness 8 Temperature Z. S A.s2S. d. 5.

p2H (S.U.) -71.q7 1;qSR' .Q3 9 s£ VI5 .o9 DO (mg/L) 8-t S. .l 8 0 1 .S 1. q 600 mg NaCI/L C"monductivity1S~O D -O

-Temperature l. 5S l. 2.C)%1 S.1 pH (S.U.) L 1° .0 1 8 .OC5 %.oS DO (mg/L.) S sl gO -7I B00 mg NaC/L Cpmonduct)140ivity l15 Tempersture -J.-S -ZS.\ 2i l5 l t pH (S.U.) co1 Xlc1 Is.3 q 'S.u4 3.C0 1000 mg hNaCIAL ConductivityZ3O3 3D

.Temperature 2.q*Y ZS. Z 2Y1 2Z. 2-P z pH (S.U.) *1.9'7 A.02 2.~o 'i.q %19o E.o1 DO (mg/L) 16. 1s. c: io 5 -9 _

1200 mg NaC/L C"monduct)ZSivOZSos fit Temperature '. 2,;_ Z Zq.9 w.7 S RH (S.U.) 19o T.S

.oZ. 1-.98 83.oZ 8.010 DO (mg/L) 7. Z _ t 2 2-Z 'l0 to s-1400 mg NaCI/L Conductivity. .

(pmhoS/cm) 3BlIRO 3 2 3 5t2.

_Temperature 2q SZ 2.9 24A zt.2Lq 25. 0 SOK Conductivity 3o (p~mhoS_. .

Inltial Final _

I nitial Final . I nitial H Frinal

Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphniadubia CdNaCLCR #: ___

Y Day p p

3 4 5 6 Anays____P___--11 Pc.s C41l CA CA3 Co-ncentration Parameter "A 4apo~ 4EeP

_pH (S.U.) cv.%, D).M~ I .O3 i.x6 7.91 l 5.0ql %.O3 'S.03 DO(mgL) 8.3 . 17.7 l__7.1 l_l.S -.

CONTROL Conductivity 30c3 (jimhos/cm) 5o _ __ _ __ _ _

Alkalinity \ \W (mg CaCO3 /L)

Hardness (mg CaC03L)__ _ _ __ _

Temperature

(°C) *25 Z-q E. SS Z;. 2A. -s.\ I 7zl S.IL pH (S.U.) . 9n4 9L.. 8. o0 _ .,O DO (mgfL) V. 0 6. I _.__ _1-7

_ - 1._ ._S 600 mg NaCVL Conductivity 1 (Pmhos/cm) 4 15_

t jqY30 Z5 90 Temperature 25l Z5.1 2.q ZS pH (S.U.) rsI .O . 7.S it ~ T-0 13.l - 2.0 DO_(_ _ 8.0 .0 __ 7 _ _ _9._

800 mg NaCi/L Conductivity =M L_

(.zmhos/cm) ______ I( 3O ___ _ _ _3>_z Temperature q 25. \ Z3 .9 2S. I Z. 21 '

pH (S.U.) 'a\iO 334 AOL.

_iS3.t '%o.67 J.y _____

DO (mg/L) 'B 9.0 4. 1Q-o 7 - \ l 1000 mg NaCVL Conductivity (pmhos/cm), Z ?.5o ____ 723-7o _ _

Temperature (IC) 7'

i-Z2. S2 t. t 5\ 2 tg lZ 7.s .

pH (S.U.) S. _____ _____ scz

'T. %.t l 8o DO (mg/L) 9 .0. 7 .1 ' - 7A 1200 mg NaCI/L Conductivity

(;im hos/cm) _ _ _ _ _ _ _ _ _ _ _ _ Z I O _ _ _ __1 Temperature 2S S.Vl. 2. Z 2. ;Z.

pH (S.U.) 0.oo1 7.4 9J _ ot 7.03 -tO _8.o_

DO(mgIL) 0 I ___z13 1.7 7.9 .O .0O -. q 1 4 00 mg Conductivity NaCVL bihSlcm)

Temperature S 01____3Zi 2 2q.* 24 *

~

24 2

STOCK Conductivhity (pFmhos/cm)

Initial Final Initial Final Initial Final Initial IFinal

PERMITTEE NAMEIADDRESS (Include FacilityNamehlocaton ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ____________

_ __ _qNTEOFFICQ E SB-0 - - - - - - - - TN0026450 103 G IF - FINAL SODDY- DAISY TN 37384 _______ PERMIT NUMBER IDISCHARGE NUMBER LOW VOL WASTE TREATMENT POND FadcjW _ A- SEQUOYAH NUCLEAR PLANT MOAITORINGl PERIODl I EFFLUENT t

Location HAMILTON COUNTY -----

FYEAR MO DAY ;IMO 1 I3DAY 0 - NO DISCHARGE = "I ATTN: Stephanie A. Howard From 04 l1 01 o0 1NOTE: Read Instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE

___ ____ __ __ ______ __ __ ___ __ __ ___ __ __ __EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE *** 7.1 8.7 12 0 18/30 GRAB MEASUREMENT 00400 1 0 0 PMI TREV-GRAB~

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NAMETrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this do unent and all attachments were prepared under my TELEPHONE DATE ractionorsupervisonInacordanc with asystem desgnedtoasssurstfattquaiersdponnel miV As7thnmlirf f.1l V.iA J. Randy Douet rroPerty gather and evaluate the Information submitted. Based on my Incuilry of th person or -'4LrJ "I. i' v_

persons who manage the system or those persons directly responsible for gatherIng Principal Environmental Engineer 423 843.6700 04 12 10 Site Vice President acate, and complete. I am ware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED nformation, Incuding the possibility of Ime and Imprisornment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPEDOR PRNTEDCODE COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference all attachments here)

The Turbine Building Sump was discharged directly to the Yard Drainage Pond on November 1 and 4-7, 2004.

EPA Form 3320-1 (REV 3199) Previouseditions may be used Page I of I

PERMITTEE NAMEIADDRESS (Include FacilityName/tocatlon ItDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No.

Address P.O. BOX 2000 ____________

(SUBR 01) 0MB No. 2040-0004

_ _ _ _1TEj0FFI(CE SB-2__ 5TN026450 107 G F- FINAL SODDY - DAISY TN 37384 _______ TNPERMIT NUMBER I IDISCHARGE NUMBER METAL CLEANING WASTE POND FadcV_ TVA -SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY _________ I MONITORING PERIOD l EFFLUENT YEAR I I DAY o I DAY ** NO DISCHARGE =

ATTN: Stephanie A. Howard From 104 1 II1 01 I To 04 1 30 NOTE: Read Instructions before completina this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

_________________ ________ ____EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.9 8.1 12 0 3/30 GRAB MEASUREMENT 00400 1 0 00400 1 0

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SOLIDS, TOTAL SUSPENDED SAMPLE 3 19 0 3130 COMPOS MEASUREMENT 00530 1 0 0 . -:VPERMITZV' ***: MGIL ; -DAILY-4 COMPOS EFFLUENT GROSS VALUE ';REOUIREM'ENT

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FLOW, INTONDUI FE ORS RU SAMPLE 0 0320 0330 3/30 CALCTD TREATENT LANTMEASUREMENT EFFLUENT GROSS VALUE . ,.NT MOAG .DIY X ____-444:¶44*!

50050 1 0 0 REORT MGD _ .*..... LY- iCALCTD.

NAMETI TLE PRINCIPAL EXECUTIVE OFFICER S Certiy unoer penalty of law that his dowznent and ali atadhents were prepared J. Randy Douet under dlrection or supervision ki acoordanco with* system designed to asssre that qualified personnel A/)1tjJ.4 (Ji1IaliB prcpedty gather end wevauate the Inlormalton swbrmtted. Based on my kiquly df the person or

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.'r Q TELEPHONE 3ry 0 DATE Principal Environmental Engineer 423 843-6700 04 12 10 Sleacc Pesdette, and complete. I am aware that there are signifiat penalties fhr submitting false SIGNATURE OF PRINCIPAL EXECUTiVE TYE RITD O normation, induding the possibilityoffin and IflplSrVsonerlt forIjowfgvoloatbrns. OFFICER OR AUTHORIZED AGENT AREA lNUMBER YEAR MODAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (ReMerence alTatachments here)

The Lined Metal Pond was discharged directly to the LVWTP (Outfall 103) on November 1-3, 2004. No phosphorous bearing cleaning solutions were used.

Pag 1 of 1 EP11AF-orm JJZU-1 (RE:V JIUV) Prewous may be editions may u,4 eb De U.se used Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameltocatIon fDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATiON SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMB No. 2040-0004 Address P.O. BOX 2000

__ __LNTERO0FFI(LE SB-?N - --- -- I TN0026450 110 G F - FINAL SODDY DAISY TN 37384 _______ PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER Ldaiy_dTVA- SEQUOYAH NUCLEAR PLANT _ _

Location HAMILTON COUNTY _________ I MONlITORINmG =PFiRI I EFFLUENT MO DAY . T IYEAR MOI DAY

^- NO DISCHARGE XX ..

ATTN: Stephanie A. Howard From 04 11 I 01 ITo 104 l 11 130 NOTE: Read Instructions before completing this form.

T I

-1 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penatly of law that this docunent and all attachments were prepared under my . . TELEPHONE DATE ldirectionor supervIsion Inaccordance with a system designed to sss fthatqualified personnel T E O DATE J. Randy Douet propertY gather and evaluate the Information submitted. Based on mn Iquiry of the person or Li4)1 - Xv perons who manage the system. or those persons directly responsible for gathering te Principal Environmental Engineer 423 843-6700 04 12 10 President Site Vice ormalion, the Information submitfted Is,*to fth best of my knowledge and belief, true,_ _ _ _ _ _ _ _ _ _ _ _ _ _ _

te ce President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE Tnformation. Indcuding the possibility of rne and Iiprisorrnent for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PR INTED I COMMENTS AND EXPLANATION OF ANY vIOLATIONS (Reference af sttachments here)

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 1 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ CO DE__

No Discharge this Period tF'A FonM J32rU-1 tREV JIVI) i- e v ou eamtons Previous ec on mayDese s may xusecf use a Page 1 of _14_

Page I of 1

PERMITTEE NAME/ADDRESS (Include FacilityNameAocatn iofDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT________ DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMB No. 2040-0004 Address P.O. BOX 2000 ____________

_ __ _jINTER09CE SB h_ ---- _ _- -- - I TN0026450 116 G F - FINAL SODDY -DAISY TN 37384 _______ I PERMIT NUMBER NMBER N lBACKWASH UDIMSCHARGE Fadlity VA - SEOUOYAH NUCLEAR PLANT - - - - _

Locaton HAMILTN COUN T___LY I MONITORING PERIOD I EFFLUENT F YEARMOiDAY ToYEAR IMOIDA

NO DISCHARGE = -

ATTN: Stephanie A. Howard From [04 1 1 I 01 To 04 1 30 NOTE: Read InstructIons before completina this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE

____EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 09A 0 1/30 VISUAL MEASUREMENT 01345 1 0 0 ,. -.PERMI, *, .. " PASS=O ' j:PSEE VISUAL" EFFLUENT GROSS VALUE i.-.

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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER J. Randy Douet I Certify under penalty of law that this document mnd all allachments were prawed under my diredion or supervision h accordance with a system designed to assurs that qualified personnel propedy gather and evaluate the lnfonnatlon submiitted. Based on my iqnuiry of the person or iLL I"'

aELEPHcDAT

,-. II TELEPHONE DATE rom the vsysin,or thosepersons edirectly responsible for gather thse l Prinbpal Environmental Engineer ARE 843-6700 04 12 10 TYPED OR PRINTED inomtoicdn h osb'iyo9rrSedisbnetlrinwn iltos ' OFFICER OR AUTHORIZED AGENT corEA Il UME YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)

Operations performs visual Inspections for floating debris and oil and grease during all backwashes.

i'rwou . ecinon may usea ._ Paget1_ of^_ 1_._

EPA Form 3JZU-14e trict JI9S} PmQVous editions may De used Page 1 of I

PERMITTEE NAMEIADDRESS (Include FacilityNametocaton if Different) NATIONAL POLLUTANT DISCHAARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT ____ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Address P.O. BOX 2000 ____________ (SUBR 01)

__1 T` OFCmM o S - - - -- -- I TN0026450 L 117 G F- FINAL SODDY -DAISY TN 37384 I PERMIT NUMBER E I DISCHARGE NUMBER BACKWASH FaciW__ WA- SEOUOYAH NUCLEARPLANT Location HAMILTON COUNTY __________ I MONITORING PERIOD I EFFLUENT IYEAR I MO I DAY I . IYEAR IMO I DAYI

NO DISCHARGE -

ATTN: Stephanie A. Howard From 104 1 11 I 01 ITo I04 1 1 30 NOTE: Read Instructions before completing this form.

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cerify under penaity of law that this docrnent J. Randy Douet direction or supervision inaccordance with asystem andaatlsTenls were prepared uider my I designed to assure that qualifiedpersonne property gather and evaluate the Information submittied. Based on my Inquiry of the person or Q.r (i_

(_ I tC TELEPHONE DATE I

SieVoePeidnrsomtons who inomanaetesyste Site VicePresident informa Worthoe @i°ns pis direcaly responsible for gathering fth Principal Environmental Engineer 423 843-6700 04 12 10 Information submitted Is,*to fthbest or my knowledge NAnbelaef. truwo.____________________

ftonth accuate, and complete. I am awre that there re significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE Pinomation, includhig the possibility offine a.d imrent for koi violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I______________CODE________ _______________

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference affattachments here)

Operations performs visual Inspections for floating debris and oil and grease during all backwashes.

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1_.l. 1_._._

CAAon EPA Forrn 33Z201 se I kCrv 31YY) i-wrou may De usea t-revidus eamuons may used Page I of 1

PERMITTEE NAME/ADDRESS (Indude Facilit Name/Locatin if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAHNUCLEAR PLANT ____ DISCHARGE MONITORING REPORT (DMR) MAJOR FOMB No.

Address P.O. BOX 2000 ____________

(SUBR 01) 0MB No. 2040-0004

_ _ _ _iNTEROFFCE SB-ZM . . . . . . . . _ l TN0026450 1 118 G F- FINAL SODDY- DAISY TN 37384 I PERMIT NUMBER I I DISCHARGE NUMBER WASTEWATER & STORM WATER fagjV_ TVA -SEOUOYAH NUCLEARPANTN_----

Location HAMILTON COUNTY __________ MONi'iITORINGr PERIODl i EFFLUENT YEAR rMo l YEAR I MO DAY NO DISCHARGE 3 ...

ATTN: Stephanie A. Howard From 04 11 I 01 To 04 1 11 30 NOTE: Read InsiulctIons before comDletno this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE

__ _ _ _ _ ___ _ _ _ _ ___ _ _ _ _ _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE ******** 19 MEASUREMENT 1 00300 1 0 0 'E PEWRr,7. 7.-zi.** **.**** 7-' MGIL s T E GRB EFFLUENT GROSS VALUE ~*&-". ZRQIEET DA-IN-vWEK'.`-

SOLIDS, TOTAL SUSPENDED SAMPLE ****** * * ******** ********_.._._.

MEASUREMENT 19 00530 1 0 0 t - PER"' 2 * - - ' - MGIL _ *TVVICE _GRAB EFFLUENT GROSS VALUE RQIEET~, ~ ? 2- ~~'-~ EK~-

SOLIDS, SETTLEABLE SAMPLE MEASUREMENT 25 00545 1 0 0 . . _* _

m -O 1' MUL GRAB EFFLUENTOGROSS VALUE _ ~ '-- ~DAILYMX ` MONTH ~

FLOW, IN CONDUIT OR THRU SAMPLE 03 *******

TREATMENT PLANT MEASUREMENT 03 50050 1 0 '-REP0RT7E.:E .D-LYREP.ORT< MGD ' i . ON-CEI; .ST EFFLUENT GROSS VALUEEUREMENT MOAVG, ____* . _ _BATCH ,DIYX .

SAMPLE MEASUREMENT SAMPLE MEASUREMENT

'EQREMENt i? .

SAMPLE MEASUREMENT

"-REQU1IREMEiN~i~ v ..- ,

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certi"y under penalty of law that thisdoacuent andan attedirrrments were prrerered uider my dr1tection or supervision I accordane with a system designed to asp" fthat quaified personnet

_ I Nr q_ t TELEPHONE TELEOINE DATE D4 ATiE J. Randy Douet r-operdy gather and evaluate the Information submitted. Based on my Inquiry of the person orr P El En 423 80 Sie rsiet ee sons w~ho rananre th syst'omd or ftste pal d rn~wty tterptsbe for godettiorin Principal Environmental Engineer 423 843-6700 04 12 10 Site Presidentt.

Vi~~e aton the onakn sulmitnited Is,1 theVi best of My lnoaedge and berief, true. _______________

Seuierse end comnplete. I em aware that there wre significant penalties for *ubrnitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED ifonrmtlon, Incuding the possibility of fine and Inprisonmdent for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference aa ettachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

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EPAy Lrn Form Ju*1 ihraBv ro_ryn J3ZU-4 Rtrv JN9) r-vous eatirons ma PrviousA_.on mayoe..u.e_

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ML043510058

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ML043510058

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Dear Mr. Hannah:

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