Text

July 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant
	ML042290246
Person / Time
Site:	Sequoyah
Issue date:	08/12/2004
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML042290246 (118)
	v • d • e

{{#Wiki_filter:Tenrnessee Valley Authority. Post Office Box 2000, Soddy-Daisy, Temessee 37379-2000 August 12, 2004 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6e Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JULY 2004 Enclosed is the July 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, )kd&k.aWcS Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555

PERMITTEENAMEIADDRESS ncude FecafftV NamAAottn ifDffDnO Name TWA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000__ _ _ _L -fQ_%0)L0& -n2e fac&_ TV -SUOAHgNUCODLEA ff.____ Lgcaon HAMILTOCUM2NTY - - NATIONAL POLLUTANT DISCHARGE ELIINATION SYSTEM (NPDES} MAJOR DISCHARGE MONITORING REPORT (DM (SUBR 01) I TN026450 101 G F - FINAL I PERMITNUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE I __,_MONIRING PIO EFFLUENT Fonn Approved. OMB No. 2040-0004 YEAR I MO I Fo TI From 04 l 07 l 011 To 04 l 07 l31l ATTN: Stephanie A. Howard NO DISCHARGE D NOTE: Read Insrtueflons blarrm k nm this fm_ PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE _EX OF TYPE AVERAGE MAX" UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 30.2 04 0 31/31 MODELD CENTIGRADE MEASUREMENT 0010 Z 0 0 re DEG. C. 7711"N 901 7KrE INSTREAM MONITORING C Ž TEMPERATURE, WATER DEG. SAMPLE

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42.7 0 31/31 RCORDR CENTIGRADE MEASUREMENT 04 00010 1 0 0 to" 27 pDEG.C EFFLUENTGROSS VALWE DEG. C. i M PH SAMPLE 7.3 7.4 12 0 8131 GRAB EFFLUENTGROSS VALUE 9.J7 SU 4 MEASUREMENT 12 00530 1 0 0 Su*-'MI *7 EFFLUENT GROSS VALUE MLtL m OILIAND OREASE SAMPLE 9***n* _5 <5 9 O 4/31 GRAB MEASUREMENT 19 0 00550 1 0 0 ElIRt< a-<w1/2 MOGV a 3/3 COD EFFLUENT GROSS VALUE FLOW, IN CONDUIT OR THRU SAMPLE a<5 1609 03 4/31 GRA TREATMENT PLANT MEASUREMENT 505 0 0 WT*******-,M .rh~ EFFLUENT GROSS VALUE MG CHLORINE. TOTAL RESIDUAL SAMPLE <0.016 0.030 1 31/31 GRAB TREATENT LANTMEASUREMENT 5006 1 0 0 -1 5 -MG-L EFFLUENT GROSS VALUE N,_ MtL Y NAMlrTLE PRINCIPAL EXECUTIVE OFFICER I CrUfytmup dw Ityoflawlutdocumw trd aN tltantawwe.ed dw my TELEPHONE DATE dhee0n oruPeIsion h ardieh asyat dsied pt 6 u ut qLid ^pwu _ A J. Randy Douet p randevaluatfoenlt nted.Based on krquryo tien a or A. SiekePrsin Mm t lobelPrincipal Environmental EngIneer 423 843-8700 04 08 12 Site Vic PresIdent ptlie ln tbsbttb false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED S ihe poq d lif ne and n l h r b OFFICER OR AUTHORIZED AGENT AREA MB DAY '.4MMUIY>t 50 MIIU tArL1wv' I Iful LFI AN T VIIULA I JNS (Reftfec aN A dfctef at) No dosed mode operation. The following Injections occured: 1. PCL-222 (max calc. conc. was 0.058mg/L-limt 0.100mg/L) 2. CL-363 (max. cal. cono. was 0.OO9mg/L4fmlt 0.lOOnmgL) 3. PCL-222/PCL-401 (max. cabc. conc. was 0.O20mg/L-4lrnft 0.100mg/L) 4. H-130M (max calc. conc. was 0.031mglr -L-nft 0.050mg/L) S. H-130M (low detection level analytical method was -0.020mg/LA-irnft 0.O50mg/L)) EPAruf XarmI IRLYe M)v rnoumIa Wuwnfm Hmy DU uses Page I of 2

PERMITTEE NAMEIADDRESS flncude Fad/htvName.caWn fDifferent Name TVA. SEQUOYAH NUCLEAR PLANT dud QP.OX B OO _ W_ n_ _ NEROFQ 2900 .B3@ ____ODDY-YE TN 37384 .E!fJ!q TV -SEOUOYAH NJILcEA -f-E-Locaonj __ECT.NTY CUNT________ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCKARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMRR (SUBR 01) I TN0026450 101 G F - FINAL I PERMIT NUMBER [IS G NUMBR l DIFFUSER DISCHARGE Form Approved. OMB No. 204040004 ll D l I u&MnnluM nco DIltfl I From TDY YoAR MD From 1 04 1 07 l 01 ITo 04 l 07 1 31l EFFLUENT NO DISCHARGE j ] NOTE. Read hsbuctn befbre comoleiln this fom PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE ________________EX ANLY TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS liALYSIS TEMPERATURE - C, RATE OF SAMPLE 0.1 62 0 31 /31 CALCTD CHANGE MEASUREMENT 8223 1 0 0 ,77-DEG C EFFLUENTGROSS VALUE -D WHR TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2.1 0 0 31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 DEW. 7 D WC EFFLUENT GROSS VALUE tUIMW7'>uos. BORON, TOTAL SAMPLE <0.2 <0.2 <0.2 19 0 1/31 GRAB EFFLUENT GROSS VALUE Illwt = 1 SAMPLE MEASUREMENT 4'

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4_. $,g SAMPLE-MEASUREMENT SAMPLE MEASUREMENT _-7.,.;' 7 m AEIRMENT 3, A" SAMPLE ~-- MEASUREMENT NAMEITLE PRINCIPAL EXECUTIVE OFFICER I C undwir ty of lawt I th doastwriid iltWli wS ipWd uiKS I TELEPHONE DATE dxdbnrhhmddbmualh-- edwhhIhyWD ^ W tK",. ' d~ J. Randy Douet J0cblraP.w.Ion In W moarduMb i a witudw cmd y to.sy. M f Pew Sie VRad President _ m m Mu iotn

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Prlncdpal Environmental EngIneer 423 843-6700 04 08 12 SiteV, sd acm I m Wdn M brP eomftqfl SIGNATURE OF PRINCIPAL EXECUTIVE TYPDI O PRidNTnE Mu hd a y p u flmwtydofn arid h 1Iiww h w b OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED i I________________________ CODE____________ COMMENTS AND EXPLANATION OF ANY VIOLATIONS p~ence 1 aacments here CCW data for July 2004 Is attached. FPA,-F. vr 110 0C Page 2 o 2 F-- .0-1 rV-nw ruwwa meay UV wem Page 2 of 2

CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 07/01/2004 0820 <0.5 mg/L 07/02/2004 1813 CVS EPA 8015B CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 07/01/2004 0815 <0.5 mg/L 07/02/2004 1736 CVS EPA 8015B

PERMITTEE NAME/ADDRESS (Incdude Feo7itvNam7aFocaton ifDietnf Name __A - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 SODOY - DAISY TN 37384 fedjly _ _A-S2U AHNqCLEAR PLANT .LcaL HAMILN OQUN T_ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM O(PDES) MAJOR Fonn Approved. DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 TN0026450 101T F - FINAL PERMIT NUMBER DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 a~fLrrfM2L ~ _ _~ I EFFLUENT 1 MW1,41 1 %.-FWII%.7-r-I From O I DAt1 YEAR MO l NO DISCHARGE D NOTE Reed IehictoM before Complet this hrm PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUIM UNITS MINIMM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1/0 COMPOS CERIODAPHNIA MEASUREMENT TP35 1 0 0 , M-MP-- 043EREN ~ EFFLUENT GROSS VALUE. ERNT z IC25 STATRE 7DAY CHR SAMPLE >100 23 0 110 COMPOS PIMEPHALES MEASUREMENT 23 TPSC i 0 0 P~

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ERCENT1/2f EFFLUENT GROSS VALUE RCENT E SAMPLE MEASUREMENT V., ~,X-SAMPLE MEASUREMENT MEASUREMENT 97Ui gMrn" SAMPLE MEASUREMENT ~VT HQUIRE EN ___7 ES SAMPLE I MEASUREMENT 'F-PER~MITt~ L-NAMEnTrLE PRINCIPAL EXECUTIVE OFFICER I C~f a pent oflo 1* IIh dmunenl nd gm l leted vi w mune mW TIELEPHONE DATE J. Ranldy Douet = OW erW nd_ _vlu~ *w _ bt nai s ubmitd pd aw _qf ft who menag Do sn, -th Prsnsdre ndyi ogem,oS Principal Environmental Engineer 43 8370 0 8 1 SKe Vioe President ftcnftN Calwnhr SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED i f kc rto;ii I IhYofisN a fond W fr bhig violatins. OFFICER OR AUTHORIZED AGENT AREA I NUMEER YiEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refrm so effheonwt heme) Toxicity was sample July 5-10 2004. Report is attached. _ME~I EPA Form 3320-1 (REV 31") Pmvfous editions may be used Page I of I

August 9, 2004 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, JULY, 2004 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected July 5-10, showed no toxic effects to fathead minnows or daphnids. The resulting IC2s values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment): Files, ER&TA, CEB IB-M SQN July 2004M

TENNESSEE VALLEY AUTUORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: August 9.2004

1. Facility I Discharger: Seguovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit#: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3.266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 2.992.4

8. Outfall Tested: 101

9. Dates Sampled: July 5-10. 2004

10.

Average Flow on Days Sampled (MGD): 1596. 1592. 1595. 1591. 1592. 1591

11.

Pertinent Site Conditions: H-130M molluscicide was injected from July 6-7, 2004. The dates and times for the H-130M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period. injection.ocation Date/Start Time Date/Ending Time Raw Cooling Water 07/06/2004 1010 07/07/2004 1009

12. Test Dates: July 7-14. 2004

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%/6): Outfall 101: 10.98. 22.0.43.9. 72.0. 100.0 Intake: 100.0 Pimephales promelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 TV treated Intake: 100.0

16. Permit Limit Endpoint (%/): Outfall 101: ICMs = 43.9%

17. Test Results: Outfall 101: Pimephalespromelas: IC25> 100%

Ceriodaphnia dubia: IC > 100% UV treated Outfall 101: Pimephalespromelas: ICQ, > 100%

18. Facility

Contact:

Stehanie Howard Phone #: (423) 843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755 j

22. Notes: Outfall 101 samples collected July 5-10, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present. 2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD[YY)/ (MDD/YY)/ Arrival Initial (MMIDD/YY)/ Sample Time (ET) Time (ET) Temp. TRC* Time (ET) ID Collected Received mL Used By 07/05/04 0746 to 07/07/04 1435 11 07/06/04 0646 070/413 .~ <.0 07/08/04 1409 Intake 07/05/04 0803 to 07/06/04 1338 1.6 <0.10 07/07/04 1435 07/06/04 0703 07/08/04 1409 101 07/07/04 0737 to 07/08/04 1300 2.4 <010 07/09/04 1400 07/08/04 0637 0/8413007/10/04 1351 Intake 07/07/04 0717 to 07/08/04 1300 2.3 <0.10 07/09/04 1400 07/08/04 0617 07/10/04 1351 07109/04 0740 to 07/11/04 1346 101 07/09/04 0740 to 07/10/04 1232 2.4 <0.10 07/12/04 1400 07/10/4064007/13/04 1413 07/0904 078 to07/11/04 1346 Intakce 07/109/04 0718 07/10/04 1232 3.1 <0.10 07/12/04 1400 07/1/04 61807/13/04 1413 TRC -Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 k 1.0LC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics. Inc.! for 2 minutes. 3

Pimephales promelas Cerlodaphnia dulbia Test Organisms:

1. Source:

Aquatic BioSystems. Inc. In-house Cultures

2. Age:

23.5-25.5 hours old <24-hours old Test Method Summary:

1. Test Conditions:

Static. Renewal Static. Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:.

4

5. Organisms per Replicate: 10 10 I

6. Test Initiation: (Date/Time)

Outfall 101 IV Treated Outfall 101

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101 07/07/04 - 1435 ET 07/07/04 - 1415 ET 07/14/04 - 1409 ET 07/14/04 - 1352 ET 07/07/04 - 1104 ET 07/14/04 - 1023ET

8. Test Temperature: Outfall 101:

Mean = 24.80C (24.0 - 25.50C) Test Temperature: UV-Treated Outfall 101: I Mean = 24.90C (24.4 - 25.4LC) Mean = 24.80C '24.0 - 25.50C)

9. Physical / Chemic Measurements:

al Alkalinity. hardness, total residual chlorine. and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one reolicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for DH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA). 4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 7-14. 2004 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 98 98 43.9% 100 100 100 98 98 98 98 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 Intake 100 100 1100 00 100 100 100 Test Solutions Mean Dry Weight (mg) (% Effluent) pcate number) (%Efun)12 3 4 Mean Control 0.705 0.806 0.641 0.660 0.703 10.98% 0.672 0.677 0.717 0.746 0.703 22.0% 0.642 0.654 0.844 0.682 0.706 43.9% 0.779 0.762 0.762 0.746 0.762 72.0% 0.795 0.848 0.780 0.779 0.801 100.0% 0.679 0.760 0.695 0.752 0.722 Intake 0.847 0.735 0.794 0.689 0.766 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7Zda Toxicity Test. (Genus species) (Type / Duration) Conducted July 7-14. 2004 using effluent from Outfall 101. Percent Surviving Test (time interval used - days) Solutions 2 3 4 5 67 (/oEffluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (realite number)

1.

2 3 4 5 6 7 8 9 l 10 Mean Control 35 35 35 33 32 35 29 36 33 34 33.7 10.98% 30 35 35 34 30 34 36 30 31 34 32.9 22.0% 32 39 30 36 32 38 32 38 35 35 34.7 43.9% 32 35 34 39 41 36 39 32 34 35 35.7 72.0% 33 35 39 36 33 35 40 35 34 32 35.2 100.0% 41 40 43 33 41 36 35 40 36 41 38.6 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TLJc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = 100/LCso: TUc = 100/ IC25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 7-14, 2004 using water from Intake Percent Surviving Test (time interval used -__as_ Solutions 1 (%MoEffluent) 1 2 l3 l4 l5 l 6l7 Control 100 100 100 100 100 100 100 Intake 100 100 I 100 I 100 I 100 100 I1 Test Solutions Reproduction (#young/female/7 days) Efflu Data (replicate number) (%______ent) 1 2 3 4 5 6 7 8 9 10 Mean Control 36 35 36 30 34 34 29 35 34 34 33.7 Intake 39 44 33 40 37 33 42 39 36 41 38.4 IC2 Value: > 100% Calculated TU Estimates: <1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = IOO/LCso: TUc = 100/ IC25 7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 7-14. 2004 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval u - days (% Effluent) 1 2 3 4 6 7 Control 100 100 100 100 98 98 98 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 95 95 95 Test Solutions Mean Dry Weight (mg) (% Efsluent) (replicate number) Mean (%Efun) 1 2 3 4 Mean____ Control 0.771 0.628 0.607 0.775 0.695 10.98% 0.644 0.701 0.695 0.606 0.662 22.0% 0.739 0.728 0.640 0.722 0.707 43.9% 0.784 0.667 0.661 0.685 0.699 72.0% 0.641 0.642 0.702 0.682 0.667 100.0% 0.791 0.796 0.728 0.706 0.755 Intake 0.687 0.724 0.556 0.656 0.656 IC25 Value: > 100% Calculated TU Estimates: <1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

TUa = l00/LCso: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time I Duration I Toxicant R Results (QC 25) Pimephalespromelas l July 7-14,2004 1500 7-days KCI l 536.9 mg/L Ceriodaphnia dubia July 7-14, 2004 1125 l 7-ays I NaCl l 1055.1 mg/L 8

PHYSICALCHEMICALESMfMY Water Chemistry Mean Values and Ranges forPimephalesppmmelas and Cerlodphnfia dubia Tests, Sequoya NuclearPlant Effluent (SQN), Outfal 101, July 7-14,2004. Test Sample D Tempetr CC) l Dissolved O.ygen(mgL) pH lConductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (junhoslcm) (mg/L CaCO3 ) (ng/L CaC%3) Chlorine (mgIL) Control 25.1 24A4 7.9 7.5 7.99 7.81 285 61 84 Coto 24.4 -25.4 24.1 - 24.9 7.7 - 82 7.1 -7.9 7.41 - 823 7.33. 8.06 258 - 302 61 61 84 U 10.98% 24.1 24.5 7.8 7.4 7.99 7.79 276 24.5 - 25.4 24.1 - 24.9 7.6. 8.1 6.8 7.9 7.58 - 8.23 7.32 - 8.03 261 - 288 2 25.1 24.4 7.8 7.3 7.97 7.79 263 22% 24.5 - 25.3 24.1 - 24.7 7.6 8.1 6.7 7.8 7.58 - 822 729 - 8.06 246 - 280 4 25.1 24.4 7.9 7.3 7.95 7.81 248 24.4 - 25.3 24.2 - 24.7 7.6 8.2 6.8 7.7 7.55 8.19 7.27 - 8.08 238 - 261 7 25.1 24.3 7.9 7.3 7.94 7.83 224 24.4 - 25.4 24.0 - 24.8 7.6 - 8.2 6.8 7.8 7.54 - 8.20 7.32 - 8.12 214 - 236 1% 25.1 24.4 7.9 7.4 7.91 7.91 202 78 89 <0.10 24.3 - 25.5 24.0 - 24.8 7.6 - 8.2 7.0 - 7.8 7.49 - 8.18 7.33. 8.16 195 - 214 77 79 88 90 <0.10 <0.10 Intake 25.1 24.5 8.1 7.4 8.13 7.89 200 80 91 <0.10 24.7 - 25.3 24.1 - 24.9 7.7 - 8.7 7.0 - 7.9 7.55 - 8.54 7.33 - 8.17 194 - 215 79 81 90 92 <0.10 .<0.10 Control 25.0 24.8 7.9 7.8 7.99 8.02 285 61 84 24.5 - 25.4 24.6 - 252 7.7.8.2 7.6 -8.1 7.41 - 823 7.41 - 824 258 - 302 61 61 U4 -84 10.98% 24.9 24.8 7.8 7.8 7.99 8.04 276 10.98% 24.4 - 25.2 24.6 - 25.1 7.6 - 8.1 7.5 - 8.1 7.58 - 8.23 7.46 - 8.24 261 - 288 2 24.9 24.8 7.8 7.8 7.97 8.05 263 22% 24.5 - 25.2 24.5 - 253 7.6 8.1 7.5 - 8.1 7.58 - 822 7.47 - 8.27 246 - 280 24.9 24.8 7.9 7.8 7.95 8.06 248 43.9% 24.4 - 253 24.5 - 25.2 7.6 - 8.2 7.5 - 8.1 7.55 - 8.19 7.47. 828 238 - 261 72% 25.0 24.8 7.9 7.8 7.94 8.10 224 24.6 - 25.3 24.5 - 25.3 7.6 8.2 7.5 - 8.1 7.54 - 8.20 7.47 - 8.32 214 - 236 100% 24.0 24.8 7.9 7.8 7.91 8.13 202 78 89 <0.10 24A - 25.4 24.6 - 25.3 7.6 - 8.2 7.5 - 8.1 7.49 - 8.18 7.51 - 8.34 195 - 214 77 79 go 92 <0.10 - <0.10 Intake 25.0 24.8 8.1 7.8 8.13 8.11 200 80 91 <0.10 24.6 - 253 24.6 - 25.3 7.7 - 8.7 7.5 - 8.1 7.55 - 8.54 7.49 8.33 194 - 215 79 81 90 92 <0.10 - <0.10 Overall temperature CC) Pimephalespwomelas Cerlodaphnia dubia Average 24.8 24.9 Minimum 24.0 24.4 Maximum 25.5 25.4 9

PHYSICAL/CHEMICAL SUlMMARY Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, July 7 - 14, 2004. Test Sample ID Temperature (OC) Dissolved Oxygen (mg/L) (HS.U.) Conductance Initial Final Initial Final Initial Final (Rmhos/cm) Control 25.1 24.5 7.9 7.4 7.96 7.75 279 24.5 - 25.4 24.2 - 25.1 7.6 - 8A 6.8 - 8.0 7.44 - 8.24 7.28 - 8.02 265 - 299 10.98% 25.1 24.5 7.9 7.4 7.93 7.77 277 24.6 - 25.4 24.2 - 25.1 7.6 - 8.4 6.9 - 8.0 7.45 - 8.24 7.30 - 8.05 260 - 291 25.1 24.5 7.9 7.3 7.93 7.81 266 22a 24.6 - 25.4 24.1 - 25.1 7.6 - 8.4 6.7 - 8.0 7.44 - 8.24 7.32 - 8.06 243 - 283 25.1 24.5 7.9 7.3 7.93 7.80 247 43.9% 24.5 - 25.3 24.0 - 25.2 7.7 - 8.4 6.7 - 8.0 7.44 - 8.27 7.33 - 8.07 217 - 265 72% 25.1 24.5 7.9 7.3 7.92 7.88 225 24.4 - 25.4 24.1 - 25.1 7.7 - 8.4 6.8 - 8.0 7.44 - 8.25 7.34 - 8.14 207 - 239 100% 25.1 24.5 7.9 7.4 7.92 7.87 204 24.4 - 25.5 24.2 - 25.0 7.7 - 8.4 7.0 8.1 7.44 - 8.25 7.34 - 8.13 194 - 216 n 25.1 24.5 8.0 7.3 8.01 7.87 201 Intake 24.8 - 25.3 24.1 - 25.1 7.7 - 8.4 6.6 - 8.1 7.47 - 8.35 7.36 - 8.13 192 - 211 Overall temperature (CC) Pimephales promelas Average 24.8 Minimum 24.0 Maximum 25.5 10

SUMMARY

I CONCLUSIONS Outfall 101 samples collected July 5-10, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present. 11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimevhales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Ceriodaphnia dubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured using EPA Method 170.1.

4. Dissolved oxygen was measured using EPA Method 360.1.

5. The pH was measured EPA Method 150.1.

6. Conductance was measured EPA Method 120.1.

7. Alkalinity was measured using EPA Method 310.1.

8. Total Hardness was measured EPA Method 130.2.

9. Total residual chlorine was measured using EPA Method 330.5.

OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KCI or NaCl.

2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephaks promelas.

Sodium Chloride (NaCi crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analnsis of Water and Wastes. EPA/600/4-79/020 (March 1983).

14

Sequoyah Nuclear Plant Biomonitoring July 7 - 14, 2004 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 9, 2004 2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 9, 2004 Towerbrom CL-222 PCL-401 CIA363; Cuprostat-H 130M mgAL 'w~~L$ mg/L

91gL, PF ml TRC X

Copolymer mgF t Azole < 0.002 o9 026" 0.009 0.011 006 0.028 ~ 0.028 O42 ~ .009 .O6 0.008 4O)2> 0.009-0.006 g 0671 0.009 0.0, 0.109 < 0.002 <~2 ~ 0.009 < 0.002

02~~

0.009 < 0.0057 0.019 0.0088 < 0.0088 < 0.0076 < 0.0152 < 0.0141 .055 O055 0.055 0.05* 0.019 0.019 0.018 0.019 0.019 0.019 0.019 0004~""j" 0.108 0.0143 '0050.020 05- ~ 0.0092 'O02~ 0.020 0.'005 < 00120 <Q.025.<k 0.020 iK<i < 0.0087

.2 0.020 0.0120

.05 0.020

.0- < 00036 '025 0.020 <00036 025 0.020-08/27/2001 08/28/2001 029/2001 08/30/2001 08/31/2001 <>'<s 1< 0.017 <0.0096 <0.0085 <0.0094 <0.0123 <0.005 O.O6 V O06 0.0s 0.021 0.021 0.021 0.020 0.021 0.020 !`0b.006`' o 0: 1 0021 1 1/2 5/2 0 0 1 ~< 0 0 04 4 11/26/2001 -<001 19 >'A040.02 00-11/27/2001 0.0137 ~'~.930.019 O07 11/28/2001 <D00089 j 0 20.019 T.0 11/29/2001 0.0132 0Q40.020. 7-11/30/2001 < 00043 >0040.02 L 12/09/2001 ~ t' <0.0042 A -M 12/O12001 <001042 ~ 12/10/2001 ~ <001042 12/12/2001 0.0128 0.024 .2 ~ .0 12/13/2001 <0.0088 ~ 9040.02 12 1 / 0 1 ~0.0134 > b 0 40.02 7-3

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 9, 2004 PCL-401 mg/L Copolymer 0.02 0.014 0.014 0.014 0.023 0.023 0.023 0.008 4

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 9, 2004 Date LSum Towerbrom XPCL-!222>i PCL-401 VL33Cuprostat-I1439M Inyoh~rt g/L txtft 3 mg/L 1/4fgL PF mgL mgU RC Phpbt Copolymer DA.MA/ tut' 06/15/2003 < 0.0045 ~ 06/16/2003 <0.037 0057 0.020 Kt0 3 06/17/2003 <008 Ai1 0.014-- 0618203c 0.0048 t0.40.014 %94 06/19/2003 <00085 > O5S0.020 -t-025 06/20/2003 < <0.0048 <IOst 0.020 08/03/2003 It <0 0050 t~~- 08/04/2003 ~ ~ <0.0050 j~00.020-08/05/2003 <0.0051 §< 057>Y\\r 0.020 02 08/06/2003 <0.0084 ~ ~5~~ 0.020 TY -Th2 08/07/2003 ~ -0.0129 fOt 0.020 ~ .2 08/08/003 0.0153 K .S-0.020 00 10/05/2003 j<0.0043 015> 0.020 r-10/06/2003 <0.0043 "O {057,<2 0.020-10/07/2003 0090 ctS; 0.020 005 10/08/2003 .<0.0106 .073 0.020-0.2 10/09/2003 ~ 0.0181 Kv.2t 0.022-12 10/10/2003 0.0183 O02< 0.024 00 02/01/2004 0.0093 <,.070.009 00124/2004.0200340.009 009 0203204<0.0034 K 96 0.009 '-t 02/06/2004 0.010524)2 0.009 001 05/05/2004 <00014492 0.009 42 02062026.0050.0140909 05/06/2004 <0.0146 4026O37' 0.0139 42 05/07/2004 <0.02210454 0.0201 0094 .2 05/08/2004 0D0146 G00 0.0213 i-05/09/2004 <'<0.010227 '.0S 0.020-07/04/2004 0.02176 oos 0.0219 07/05/2004 M<0.010854 O 0.020 00 07/06/2004 <D000785 O~ 0.020 03 07/07/2004 ~ ~ 0.0252 7056V 0.019 <03 07/08/2004

37 0.0223 7

7 0.0 19 00 07/09/2004 70.0182 0.020 00 5

Sequoyah Nuclear Plant Biomonitming July 7 - 14, 2004 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1 of 3_ Client TVA Environmental Testing Solution, Inc. Deliveed By (Circle One): Project Name: Seqoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Nmnber N/A Asheville, NC ot (specify): Expre comier Facility Sample Sequoyah NP 28801 General Comments: NPDISNumber TN0026450 Phone: 828-350-9364 At &0 < CollectedB yWanda Alen Fax 828-350-9368 Field Identification I C (ib/Camp. e): D R cine Flow goo. Sample Description Niumber & (MOD Reain Event? volume )(Mark as Appropriate) Collece w.d 7/0..t"1. DeTWOe Yes NfYe. No Truce ~ 4 .e~ SQN-0-TOX Comp 7/5/04 - 7/6/04 92(2.5pl) MA SQN.INT-TOX Comp 7/5/04 -7/6/04 09qA/0V 1..5 1.N sample Ctoy-FMf In From Top Down Relinquishted By (Signature): Daterhme Received By (Signature): Date/rimne Wanda Allen 7/6/2004 7/6/2 004 Express Couder 7/6/2004 7/6/2004 l " tBructions-Clients should fill n all area except those in the 11aboaty Use" block Biomo ng samples are preservd by stoing them at 6C and spping than in ice. The hold time for ech sample is 36 hours fiom the time of. cllection Theadore, please collect and ship in aud a way that the laboratoxy will receive the samples wiYt ample time to initiate testing witbin that time fime Samples shipped ovught an Friday via FedEx or UPS most be mared for Saturday delivety or ty will not arrve until the following Monday.

Environmental Testing Solutions, TIM panI Whole Effluent Toxicity Sample Receipt Log Date

Um Red Retieyd e

SOWle Projet Sample Smple none Ead desripteio Comests Ide'eted eeds by f tep c (C) e bee. _-06-4 1356

1. Slmner _

5.0 120 040706.01 Eu ta DtvisNC 8Sapl Red w 4-h of colon 07-0604 1338 J. Sori

1.

1321 040706.l TVA-Sqdeh NuclearPlnt. Oual 101 N 01.06-04 1338

1. S3ayer Comw L6 1321 040706.03 TVA - S oh N arPlant-Witk TN 07-06-04 1630 J. S.umnr D. Ellkd IA 1.322 040706.04 _ro Caroknss - _heb4ib Ash Pend NC 0740 GE

BIOMONITORING CHAIN OF CUSTODY RECORD Page _2_ of _3_ Clien TVA Environmental Testing Solution, Inc. Dmroed By (Cirrle One): Proect Name: Sequoyah NP Todcity 351 Depot Street. Fod1tx UPS Bw Client P.O. Namben. N/A Asheville, NC oti (sp*): Express Corier Facility Sample& Soquyah NP 28801 General Comme: Cu.40e(i5.kd.S-g~LiA~ t NPDES Nwmb TN0026450 Phone: 828-350-9364 f Collected By Wanda Allen Fax: 828-350-9368 Field Identification Grab/Cmp. Conllction Daterime A tner Flow Samiple Description, Number & (MOD) R~nEvmVt .Mr K as Appropriate) A Z Ti me es Y% NO To = SQN-101-TOX Cbmp 7n104-7M04 o qh3 2(2.5gOl NA_ SQN-lNT-TOX Comp 7/04 - 7/8/04 51,5 gl.) NA Sample Cu - Fil In From Top Down Relinquished By (Signature): Dateim Received By (Signature): DateTime Wanda Allen 72004 Expss Cu 7M22004 Express Counier 7/2004 E' m ETSu 5, 7M200 100 7 Instuctions: Clients should fin in all ams except those in the 'Laboratory Use block Biumonitoring amples we preserved by storing tham at 60C and shipping them in ice, The hold time for eah sample is 36 hoirs ftom the time of collection Therefore, please collect and ship in muh a way tht the laboraoty will receive the samples with ample time to i tie testing withinthat time fime Samnples shipped overnight on Friday via FedEx or UPS must be marted for Saturday delivery or they will not arrive until the following Monday.

EmlromnentlTeatng Solutions, Inm. Path Whole Effluent Toxcity Sample Receipt Log lnedv aw

7Ir maOIr1 0

I0"J s n 14 3mh rjmtSape apha"dudp IN" 074044 0941 J. - S a-El 132 04070 101 _ _- _M "IN 4 i 2300

'evOum b

_ 07"4 US 10 K K 040702.03-WVA-:5yubNUckuPkli .o b 07e 4

BIOMONITORING CHAIN OF CUSTODY RECORD Page _3_ of 3_ Client: TVA Environmental Testing Solution, Inc. Die By (CrcD One): Prect Name: Sequoyah NP Todcity 351 Depot Street FedEx UPS Bus Client P.O. Numbei N/A Ashville, NC o ( Express Courier Facility Sampled: Sequoyah NP 28801 General Coments: NPDES Number. T40026450 Phone: 828-350-9364 4 omr SeQ Recerez CollectedByrWandaAllen Fax: 828-350-9368 Field Identification I Orab~Cmp. iCollection E~eErnt? 94PW'nf .n.w Numbes cMidtion ON (MOD) Rain Even SQN-101-TOX Comp 7/9/04-7110/04 2(2.5g) NASga ) NA SQN-MT-TOX 7/9o4 -7/10/04 YD.... Saple Custody - Fill In From Top Down Relinquished By (Signatu): DateTme Receiv By (Signatur): Datefllie Wanda Allen 7/10/2004 Express Courier 7/10/2004 Express u [70 H'1 711t2 Express Counier. 7/=0 12az Erm r-4 L"P7nno4 7110/2004 132 lnsuctions: Clients should fill m all areas ecept thse in the "Laboratory Use" block Biomonit g saTples are preserved by torg them at 69C and shipping tm ioe. The hold time for each samnple is 36 hours frm the time of collection. Theoroe, please collect and ship in such a way that the laboratoiy will receive the smles with ample tine to iniiate testng within that time flume. Sapes shipped overnight on Friday via FedEx or UPS must be markd for Saturday delivey or they will not arrive util the following Monday.

Envirenneidi Testin Sdu6lem I'm Whole Effluent Toxidty Sample Recelpt Log Dm _h l Ro Ivd Reird mpG. P;;3 m.... Smpk m d dinuCpd.. 07-1004 0942 0K73 H_1_OI M Ei1__. StMRPNC 0-100 2 2.3 1 04a7 10.02 C Ste_ S C 07.10o04 0942 .r Ke Fed-E 3.7 1325 04071M.01 Hawhy ePadMcCmo*etwuehyitd= N _____C 07.10.04 0942 K 1 Cm M. Ex 3.6 1326 04010Q04 Duhe w Cmcrslon - Fb4 ud SftmSIo lie 01-10.04 0953 UPS Q7 1329 0M47EL.03 T2M be. - P C_ W8A NC 07-10404 e953 05S Q4 1040710.06 mid 1 le - ML 0o p l_ Com 07-1004 1232 L _nm 2.4 1321 04Q7100 TVAVA- - Nb Sa. Om lol 01 .10 C4 1232

1. L W

e i 3.1 1311 047 1f011 TVA -Se Nuce SW=. Nugk ST oN 07.104W

+ Environmental Testing Solutions. Inc. Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: IVA Faciity: Seauovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project#: 13231 Dilution vreparat In ffo rmation: lComments: Dihition pV (%) 10.98 22 43.9 72 100 Effluent volum (niL) 274.5 550 10975 1800 2500 - Diluent volume (nfL) 2225.5 1950 1402.5 700 0 Total volume (niL) 2500 2500 2500 2500 2500 Test organsm Information: Test Information: Orgnism aMe: I 25.5 ro 2-5. - A4S OA b Randomizing tenylate: uelln4 Date and times organisms 01 0.OL4 J3 lb I6 EST Incubator number: were born between: o Organism source:

01.

nO-Otk AS% 6fTCA Alremla lot numbern erIO6sS Transfer bowl information: pH - Temperature - C Total drying time: Z4-cs I.-S_ 2'.S Date/ Time in: C-a-13-c 1L4&0 Average tinsfer volum: Date / Time out 61" rA!5o Oven temperature: W0' C Daflyfeeding and renewal Information: Day Date Mornalng Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time 0 pD-0 4 a 43sO0OzI-O ___DQ S &SS sao J40Q Oi-ow-0o' Dq~oiczFi.0o A( 2 O 2S rsb Y4MO 01-ot3O"OO.M 1.05 AZ a Ol S .25&n. I 0S C-OW-04 3NMOe.OzI. 03 494 t-- >t SCo 1346 0:V 4Nio,V 0>w~ta.r.e SP INL Ob i;S2 NCO 0106tA 6YO-M.01 /.00oe KEY 6 Isis 9 lS2S I4S O-ID.o4 0-I.06 V 7 X-k X,,,,,.;;K,, aLXq ORX Control information: AI ptancc c Summary of t en dpolnts: %Mortlitr. I _ 7 SW% 7-day l>o 1Ž Average weight per initial larvae: 6.105

1. f NOEC Itbl Average weight per surviving larvae:

O.,101 2 0.25 m5e LOEC CbV >h MMcf IC2 j >: I El

Environmental Testing Solutions. Inc. Page 2 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: O? t) O Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I l K L 1 o ,1 10 10 101to 1to I ,o t o to 1 1O 10 10 101 to 10t to 10 10 (ID .2 10 IOg Io ) 10 j0 1 O 10 IC 10 /O 34_ 1_ to to /( 0 /O /0 10 1O /0 /O /O to O 1/ to. /O t o to to /O 10 cto 1/ /O/ 10 /81_16 /8/c / 0/O 6o JO /O /O /0 'o 'o 10 q /O /C 7-~sd~( 1010 t0IC) 10 °)/o, q ,,0 A - Pan weIght (mg) B - Pan + Larva weight _~" i~ Lasr v a cw ve lgb (mg) - A - 2 I e Wegt per Initila nmber of 6 4A 0* 0 mCIInItlaI umbereflarvae O O'U 0 0* 0 Average weight per Initial aumber flame (mg) O.a705 0:105 O.70& Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Page 3 of 6 Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimephalespromelas Client: TVA J Sequoyah Nuclear Plant - Non-treated Date: f70t2 Survival and Growth Data Day 43.9% 72% 100% M N l OT l.R S T U V W X /0IO 10 it) /to 10 10 /o t /0,o 1

10.

10 lO J0 10 lo l0o 0 ID 10 10 /0 2 t1 tO /1t (c) /a /0 O 1_ 3_ 0 to /O -D /01t o0/0 _0 _0/0 _I S_ / 91 10 /' 1 10 /0 /0 /0lO IL 61 LA/0 I 10 10 1o 10 10 (0 - o 7 /0I I> to lO 1o 10 _ 10 10 _ o 1-la ig ( ) IL) too 1 I_ A - Pan weight (_m 3mIazi+ Lmarveweight Larvaeweight (mg) -A-B ~ ~ ~ ~ Weight per Iaittal aszmber of ,~L L,**e f, S j.arwae ((g %UlV -ClIntlala*mbereflarvae 0' 0O O' O 0 Aveage weight per hItil anniberdsbre(mg) OW1L 0 t Calculations and data reviewed: I. I I I I Z, Comments:

I I El Environmental Testing Solutintn TInr Page 4 of 6 Species: Pimephalespromegas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: M -nIn 4 Survival and Growth Data Day 150% Intake Z AA BB 0 ..1 /0 /O 1 /0 /O lo lO 2 /I /0 16 /0 3 /O to 1O 1t 4 _1010 /0 (O .S / 10 16 16 6 to to 10 /0 A - Pan weight (mg) o 1b B - Fan + Larvae weight (mg) O tx O,-* Z Larvae weight (mg) - AD-B ox I th Weight per Initial number of 4 A larvae (ng) 4A-4 - C 1 lnitial number ot larvae OO 0 O 0 Average weight per initial number or larva (me) O.-o) Calculations and datarviewed Comments: I

TVA / Sequoyah Nuclear Plant - Outfall 101 Non-treated Chirnfe Whole Effamat Teft Test WEA41-R.02-013, Metid 1090.0) Spedys: PImhahspremday Quslity Central Verfftion of Data Entry, Calvaisffens, and StntWIraAnslym TVAISsq.syS Bud's? P1ud-Wu'sut.( Twtis-u 1*144,2604 _rqw : mm 1321 ltw~br4wqmtEe -gh ht _- -t4_ A 10 10 14.95 22.00 7.05 0.705 0.705 Co B 10 10 15.00 23.06 3.06 0.06 103t.5 0.06° 166. 9763 tQS l6y C 10 10 15.10 21.51 6.41 0.641 0.703 0 0.641 ie* 073I eap~o D 10 10 15.30 1.90 6.60 Q660 B 10 1O 14.3t 21.9 6.72 0.672 0672: I9,9F 110 0 14.53 21.30 6.77 0.677 0.703 5.0 0.677 1IIQ 10" o 10 10 1 14.53 21.75 7.17 0.717 0.717 H 10 10 IS.09 22SS 7.46 0.746 0.746 I 10 9 14J86 M1.2 6.42 0.713 0.642_ K 1 10 14.39 23.33 3.44 OU4 0723 11.6 0.63544 976 3. 10 14.S3 6.t2 0.62 0.632 M 10 10 14.U4 22.63 7 .7 0.77 Q779-o&% I 10 I t 14.51 22l15 7.62 1 0.476 0.733 5.7 0.762 7 &6 i lo 1 0 1 0S31 2213 7.62 O o 7 0.762

7.

9.762 LS 44 P 10 10 _15.04 _22 7A46 Q146 0.746 10 10 1 4 S 2251 7.95 0.795 0.795 7 R 10 10 14.76 253. BAB 4.1 OS4S. 8 10 10 14.76 22.56 7.80 0.730 410.340 T 10 10 15.24 23.Q3 7.90.779 0.77~9 U 10 10 159 017 6.7S 0.619 0.679 -U 1% V .10 10 143 224 .0 0.760 o5 .s 0.760 lots 6.722

5.

4.A W 10 10 15.06 -226.9 0.695 0.695 X10 14.932

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TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Statistical Analyses L1r1 Ish Gnrwth and 9urvIm Twt-7 Dy Groth Stait Date: 7/7/ZoO4 Test 1D Pp.RCR Saile 1> TVA / Sequeyh Nuclear Mlat - Outfall 101 End Date: 7/1004 Lab MD ETS-Envir. Testing SoL Smvle Type: DMR-Divchage Monitoring Report SanVle Date: PtotoooL CHRONIC-EPA-821402.013 Test Species PP-Pirpbles promelas Comnfn entm ON-TRRATED Conc-% 1 2 3 4 D-Control 0.7050 0.8060 0.6410 0.6600 10.98 0.6720 0.6770 0.7170 0.7460 22 0.6420 0.6540 0.8440 0.6820 43.9 0.7790 0.7620 0.7620 0.7460 72 0.7950 0.8480 0.7800 0.7790 100 0.6790 0.7600 0.6950 0.7520 Transtfm: Untrauforned cOne-% Mea" N-Mean Mean Mltn Max CV% N D-Control 0.7030 1.0000 0.7030 0.6410 0.8060 10.487 4 10.98 0.7030 1.O000 0.7030 0.6720 0.7460 4.983 4 22 0.7055 1.0036 0.7055 0.6420 0.8440 13.301 4 43.9 0.7623 1.0843 0.7623 0.7460 0.7790 1.768 4 72 0.8005 1.1387 0.8005 0.7790 0.8480 4.060 4 100 0.7215 1.0263 0.7215 0.6790 0.7600 5.613 4 1-Ti t-stat CrH 0.000 -0.064 -1.515 -2494 .0.473 iBed 0oftln teal MSD Mean N-Mean 0.7349 1.0000 2.410 0.0942 0.7349 1.0000 2.410 0.0942 0.7349 1.0000 2.410 0.0942 0.7349 1.0000 2.410 0.0942 0.7349 1.0000 2.410 0.0942 0.7215 0.9818 AMdMM Teats Statitm Critical skew Ku8t SbphFo-WiMk Tes indicates omuI distribution (p > 0.01) 0.90164709 0.884 1.27209847 1.88498869 Batleftt Test indicates equal variance ( - 0.07) 10.0637131 15.0862722b d Hypotheds Teat (1-tf, O.05) NOFXC LOEC ChV TU MM)Q mMb Dannett's Test 100 >100 1 0.09423493 0.13404685 0.00647958 0.00305788 0.10993567 5,18 Treatments vs D-Control sqn_07-07-O4data

TVA / SCquoyah Nuclear Plant, Outfall 101 Non-treated Statisdcal Analyses JbwJb*"riffftuI"Rumnkv) D AS% CaThO) sow MU 1200 ICU XMO 05 >100 >100 >100 >100 >100 >100 >100 0 20 40 60 so 100 120 Do" % D..e-Reqm lt no. I 0.7 &7 T 1 0 1-WL 0.05 Wm Of fnfw"i I 1 0 2 0.1 a,I9 U sqn_07-07.04data

TVA / Sequoyah Nuclear Plant, Intake Non-treated Statistical Analyses Larval Fish Growth and Suvil Test-7 D" Growth Start Date: 7/7/2004 Test Mt PpFRCR sanmle MD TVA I Seqoah Nuclear Plant - Intake End Date: 711U2004 Lab Mt SEnvr. Testing SoL Sanvle T1e: DMR-icharg. Monitoring Repot Sanple Date: Prolcol: CHRONC-WA-821-R-022013 Test Spedes PP-Pinopbalos promelas Commnents NON-TRIATED Csne-%

1 2

3 4 D-Control 0.7050 0.8060 0.6410 0.6600 100 0.8470 0.7350 0.7940 0.6890 Transformu Untransformsed CORe-% Mean N-Mean Mean Mh Max CV% N D.Control 0.7030 1.0000 0.7030 0.6410 0.8060 10.487 4 100 0.7663 1.0900 0.7663 0.6890 0.8470 8.990 4 1-To t-Stat CON -1254 fled ISOtimh leat MSD Mean N-Mean 0.7346 1.0000 1.943 0.0980 0.7346 1.0000 AnuOHary Tests Statistie Crieal Skew Kuirt ShapliO-Vrkb Test i"cates nonrml ditnbution (p > 0.01) 0.92910469 0.749 0.54944252 -1.1099798 F-Test ediates eqnsl variances (p -0.91) 1.14550662 47.4672279 Hypotheds Test (1-ftt 0.05) MSDO mgDP MSD MSE F-Prob df Hoemocedastc t Test inicates no gnificant differences 0.09803075 0.1394463 0.00800112 0.00509013 0.2565693 1,6 Trembments vs D-Control sqnO7-07-O4data

TVA / Sequoyah Nuclear Plant, Intake Non-treated Statistical Analyses UmWktv,,, ms (l R-any) SBy vl.h gm 9% CbSS a mcs YIcO MCS IC15 J,20 X50 >100 >100 >100 >100 >100 >100 >100 4 n. as0.8 0.7 a 0.4 CK 0.2-0.1-0.0 41 .2 Z I 0 20 40 so s0 100 120 Doe % D-'R P e Im no - 0.7 I 0.31 0.2 0.1 &I d

14. 0.05 lew oCfsikwm 01I sqn_7-07-04data

EnirnenalTstigSoltos Inc Page I of 7 Environmental Testlg Solutions, Inc. Page lof 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnla dubla Client: fVA Facility: SequoYah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project#; 1521 Dilution Irearation fnormation: Comments: Dilutionpep(%) 10.98 22 43.9 72 100 Effluent Wolu=e (mL) 274.5 550 1097.5 1300 2500 DiiUntvolume(mL) 2225. I 1950 1402.5 700 0 Total vohme (E)L) 250 2500 200l 2500 2500 Test organism Information: Test Informaton: l Organism age:

£ s-nmizing template:

G 6 1 Date and times organisms on.Cn.ej csnL4O -M itl Incubator number and were born between: shelf location: 2 B Organism source: Mr-- t-CYCTbatch: 1e5 -cki-i Transfer bowl information: 2.pH -1.2 Temperature - V.'I 1 Selenstrum batchAAs W.z< tH Daily renewal Information: Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 20 n qp m.q lgC O4 VOi.OZ. l As 3

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I O j (1 lCt.oq oD706.OZ;. 1.03 4 ___1__ I OO 0 .o-OIWCf 01 iot.1 0T A3 5 O?.IZ-Dq 111 0 0-6-01 C 10. I co.t8 1 _ 6 __1___ I0 m10.1.0.m J F7

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A L. Control Inform ation: Acceptimce crtefa Summar of stendpoints: % of Male Adults: T V. I CffZ s20A 7-dayI.C50 L>CS % Adults having 3d Broods: /1 z 50% NOEC 1007., Mean Ofspring/Female: 2.1

11 a 15.0 offisrngea ChV

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Environmental Testing Solutlons. Inc. Page 2 of 7 i I I. I 1. I 1. 1 I I" 1:. Ii. i Species: Ceriodaphnla dubla Client: Seauovah Nuclear Plant - Non-treated Date: 0J; 4 CONTROL Surnival and Reproducdon Data Replicate number Day 1.. 2 .3 4 5 6 7 8 9 lo I Young produced

0) Q Adult mortality U-U-

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U. 7 Youngproduced 1 IT l i TIq i's5 L 1 In 1 Totalyoungproduced -SS T5 b a 7T 3S z9 l36 33 34 Fnnal Adult Mortality L 7 I > I 7 ? l t u X for 3' Broods t r X L § zC 1 Concentraton: l % Mortality l O7, l MeOn O ngAFernale:m _ 1 CONC: 10.98% Survival and Reproducdon Data I Repllcate uumber Day l I 2 3 4 5 6 7 8 9 1T 1 Young produced l Adult mortality 4U. .U 2 Young produced 2 Adult mortaity L L I( -m-I.- L L 3 Young produced I ) 0 L 0_ Adultmortaliy L L L LL r 41 Young produced LIC) .,~ .( JIL L14 to Adult mortality ~ L JL L L-L- t-s Young produced lo 9..7) A.L c..). -0 0 S a Adult mortality I L U L - L-U. -U U. U -U t ?- U. 7 Young produced ICAjj 1 1 ,J1j 8 FItF 1 Total young produced

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Environmental Testinty Solutions. Inc. Page 3 of 7 Species: Ceriodaphnla dubla Client: Secuovah Nuclear-Plant - Non-treated Date: CONs 22% Survival and Reproduction Data Replicate number I 2 3 4 5 6 7 3 9 10 1 Young produced 0 £ ) f C) O O E3EElm Adult mortality L-t-~L...~ L-ongpodcd ) U Youngproduced f) l. ) c) Adult mortality L t-L L ( 3 Youngproduced CI C I OO Adult mortality r L. I L.. 4 Young produced S11 Is Is Y. 5 S Adult mortality 1-l W L - X L S Young produced I to to lS lO I E I ZI O Adult mortty I _W. 1. 6 Young produced. O I 0 Q Adult mor~trlt

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68 i S 5 5S Fial Adult Mortailty 1, 1 t i Concentration: %Mortality: l Mean Offspring/Female: l 1.-1 % Reduction from Control: - S.07. CONC: 43.9% Survival and Reproduction Da IRepltcte number Day 1 2 3 4 5 6 7 a 9 10 I Young produced Z) -n a I7o Adult mortality l Ll-L L-L. l _L -XL 2 Youngproduced l f__-0 0 Adult mortality l L L L.. l-L l l L l 3 AdlYortlt J~ ung produced Adult mortality L. L ll l LW l l l l Youngproduced 10 j3 lJ .L 0___ Adult mortality L L-L L-L l-L Ll L-L S Yonng produced l0 I3Z 16 85-I I ll Adult mortallty L-l L-l l L L-U

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l l l 7 Young produced 1 ( J~i I,20 11 Total young produced aZ 3 a'! a, q 1~ 31 I, -9 2~. jq B53 Final Adult Mortl ET I ~ I L.= L ~I L-Cncentraton: % Mortali t: lMeanOffsprnw/Femnale: l 35.11 I % Reduction from Control: I -SA7I I

Environmental Testine Solutions, Inc. Page 4 of 7 Species: Cerlodaphnla dbla Client: Sequovah Nuclear Plant - Non-treated Date: Oif. L.. CONC: 72% Survival and Reprodudion Data lReplicate number _D2y 2 779 1 Youngproduced 0 O 0 O l 0 0 a Adult mortality C. I-__- V.- -l 2 Young produced 0 0 ) C) Adult mortllty L (, 1 ( .L L L-3 Young t produced. -CL> Adultmort-ty L

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AlulAdultMortality lLl L L 1-l U. l l-wxUA.veC i I. I Concentration: % Mortality: - o Mean OffpnngFemale: Iat- 'Y Reduction from Control: - 14.52

Environmental Testing Solutions, Inc. Page S of 7 Species: Ceriodaphnia dubia Client: Senupoyh Nuclear Plant - Non-treated Date:... .n 7-IDL CONTROL Survival and Reproducdon Data lie Ilcate sobe Day 1 2 3 4 5 6 7 a 9 10 1 Young produced _L C) O 0O Adult mortality L__1 2 Young produced Q V 0 0 I O O Adult mortallty I J s L L C L 3 Young produced C Adult zortallty 1 L L I 4 Young produced %W S to Sc Adult mortality I L. l -1U C.. -1 L S Young produced It I2 to 1VI ta Adult mortality L-L 6 Young produced Q Adult mortality C L 1 L i l 7 Young produced IrT r I S Zo Totalyoung produced A 5 34 ZL( z _35 __ a Final Adult Mortality t L I C. l Concentrad on: % Mortalii 0 Mean Offspring/Female: 33.1 % Reduction from Control: _P, orNc: 100% Intake Survival and Reprodaction Data i-teumbe l_Day II 2 3 4 5 6 7 9 10 I Young produced cl.= = L. Cl) £ llC l lAdult norhality L UL 4 L-L-w1e1W 2 Young produced = { mm.. -C °°l Adult mortality L j C.LIC _3 _Yung preduceda 0 ) To O Adultmortality I tL ,t L l L I L -L1 Young produced LILI I-Adult mortality L s Young produced l S l -t-7oI Adult mortality L l L C L-S Young produced C)I fl vs 0 I Adult mortality t - L t 6 Young produced I 1 19 0 0 .I I'CA'IMa< Total young produced z°q I qq1 .5 q I 3 -1 I a3 b IN &LZI si I 5i. I q I-1 eJ - -IL-I I C I C-1 L - 11 CI -J CI C I Concentration: I % Mortaliw. 1 1. Mean Offsprin emale: I I % Reduction firm Control: I - I5.I;

TVA / Sequoyah Nuclear Plant - Outfall 101 Non-treated Verification of Cerlodaphnla Reproduction Totals Control-i Dby 1 2 3 T4 1 S 7 8 1 9 1 Tl O U O O O O O O O O O 1 0 0 0 0 0 0 0 0 0 0 2 0 0 00 @ 0 T i ~ T o 3 0 0 0 00 0 0 0 0 0 0 4 T5 4 5 54 5 4 44 4 5 1 15 12 12 11 10 10 13 12 13 119 02 +/-2 0 10 00 1 7_ .i 186 _1 19 1917 1 73 Tel.! 35 3535 33 32 3523633 34 337 10.98% R- *hme S Day 12 3 45l lO " O O O U 0 1 a 1 ° 0 0 0 0 0 0 0 0 0 0 0 0 2 0 TT O TO T TT 6- 0 0 4 5 5-5 6 5 5 4 5 5 3 50 10 0 01 11 14 o 115 ~ s T T T so OO°7°1°° sT0 0 oi 0 0-00 0 7 15 is 17 18 14 18 18 15 14 17 164 T33 3 35 35 34 30 3433l 31 34 329 22% Day 2 3 4 6 7 TTW '11 1 0 0 0 0 0 0 0 0 0 10 0 2 0 O 0 00 0 0 0 0 0 0 3 0 0 0 00 0 0 0 0 0 0 4 5 5 4 5 5 6 5 5 5 6 51 12 13 10 11_L10 14 121 10 10 119 0 0 +/- 0 +/- 0 0 0 7 1s 19 16 20 17 113 18 i 20 19 177 TOWm 32 39 0 3 32 38 31 38 35 35 347 43.9% -a

S 6

7 8 9 S I o 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 00 0 TTTT 0 3 000 00 0 O 4 56 4 6S-5 5 5 4 6 51 5 10 13 14 14 16 12 IS 11 10 12 127 6 0 0 0 0 0 0 0 -if-i--i 0 7 17 16 16 19 20 19 19 116 20 17 179 Tel. 32 35 r34 39T 4-1 -34 3 ~32 34 35 357 72% 1 2 3 4 +/-5 7 I a T10e ! 1 0 0 0 0 O OI ° I O 2 00 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 6 5 5 4 5 4 5 4 4 46 5 11 13 14 12 15 10 16 13 131 12 01 0 0 0 0 2 0 0 0 0 2 7 W16 20 19 14 18 20 F 1 17 18 177 TOW. 33 35 39 36 33 35 40 35 34 32 352 100% 7D A 5 6 7 is S Tl I -- -0 -0 0 21 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 4 4 6 6 4 5 5 4 5 6 3 30 S 116 14 15 10 17 15 13 15 12 15 142 6 0 0 0 0 0 0 0 0 7 121 20 22 1 19 16 1i 20l 1 194 TOtW l41 40 43 33 41 3l 35 40 34 41 306 Control-2 SM 1 1 2 3 5 4 7 a 9 le 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 o 0 0 0 3 0 0 0 0 -0 0T T T 0 0 0 4 4 5 5 5 4 6 T T 7 4 47 5 14 11 12 10 10 11 9 0 13 11 101 0 0 0 0 0 0 130 0 13 7 19 19 13 20 1713 17 17 191 176 Tedl 34l 35 34 30 34 34 29 35 34 34 337 100i/ Intke a S S - TOW.- 1 2 3 4 5 6 I7 a 9 Tlte I 0 1 ° I o I o I o0 o l 0 ~ 1 0 0 0 0 0 0 0 0 0 0 0 3 000 0 0-0 0 00 0 4 4 -6 4 3 S 4 6 5 5 5 49 5 I15 17 13 1 12 13 15 0 13 16 115 1 0 0 0 1 0 31 7 120 21 16 19 20 16 21 11 18 20 189 TOW. 1 39 44 33 46 37 33I 4 39 34 41 3d4

TVA / Sequoyah Nuclear Plant - Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ccriodaphnia dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Clien TVI Seueyuh Nuclear Plant - Nonoveated Teft datea July 7-14,204 Project nubern: 1321 Reted byO 10 6 R-e Snunival Average repMutlo C-Ddwf d Pee aruef -f (%) 1 2 3 4 5 6 7 S 9 le () (Offa"fe"mae) I lb (%) POW gteg CA) cntrol-I 35 35 35 33 32 3S 29 36 33 34 100 33.1 6.1 N_ tappicable 10."% 30 35 35 34 30 34 36 30 31 34 lo0 32 7.2 2. 22% 32 39 30 36 32 38 32 38 35 35 100 34.7 .9 -3.0 43.9% 32 35 34 39 41 36 39 32 34 35 lee 35.7 89 72% 33 35 39 36 33 35 40 35 34 32 100 35.2 73 4,5 100% 41 40 43 33 41 36 35 40 36 41 100 39.6 S.6 44 Control - 2 36 35 36 30 34 34 29 35 34 34 100 33.7 7.0 Not applcable 100%Intak. 39 44 33 40 37 33 42 39 36 41 100 384 9.5 -13. Dwvmetta MSDvahe. Dwmst MSD valuce PMSD: 2742 7.1 MSD-bMinium SignifantDifference PMSD - Prcentm Minimn Snficant iffaerce PM SD is a easwereof fepercistpi Tb. P4SD is em m pecen diffrecn the cnitrol and W_ tmtnt tat ca be decared atatiaticalby intficktin ha whole effleenjt 0nfty s.-O averge a ugwrfl tdiffamuneoocmu for Eivromnmtal Tening Solutioms In chonic todcity Wtea wen a ocantnreucs CelrodaphWa reproductionby 10.1% from the control. Lower PMSD bound dee by USEP A (10* perentfte) - I 1% Upper PMSD bound dermed by USEPA (90 percentile) - 37% The lower and upper bounds were calculated by the USEPA osfng 393 tes conducted from 33 labotteres for Clodphenkreproduwton in chronc rdeence tndcantl USEPA. 2000. Undebanding and Accounting fbr Method Varnbity in Whole Effluent Toxicity Applicaions Under the National Poltant Discdhge Eniination ProgranL EPA-833-R0OM03. US Environnental fPction Agenc, Ccnai OI.

TVA / Sequoyah Nuclear Plant - Outfall 101 Non-treated Statistical Analyses CedodaphuIla Survival and Reoductio t-R Start Date: 7i7/2004 Test 1D CdFRCR S ple MD TVA I Sequoty Nulear Plant - OutfNll 101 EBd Date: 7/1412004 lab 1D BTS-Bnvir. Testing SoL Sarple Tye: DMRI,- eige Monitoring lRepot Saune Date: Potocol CHRONl-EPA821-R.02-013 Test Species CDCeriodapia dubli Co menbt NON-TIATEAD C;eu-% 1 2 3 4 5 6 7 a 9 10 D-Control 35.000 35.000 35.000 33.000 32.000 35.000 29.000 36.000 33.000 34.000 10.98 30.000 35.000 35.000 34.000 30.000 34.000 36.000 30.000 31.000 34.000 22 32.000 39.000 30.000 36.000 32.000 38.000 32.000 38.000 35.000 35.000 43.9 32.000 35.000 34.000 39.000 41.000 36.000 39.000 32.000 34.000 35.000 72 33.000 35.000 39.000 36.000 33.000 35.000 40.000 35.000 34.000 32.000 100

41.000 40.000 43.000 33.000 41.000 36.000 35.000 40.000 36.000 41.000 Transfrm: Untransfarmned 1-Tailed Isotonle CoeC-%

Mean N-Mean Mean Mnn Max CV% N t-Stat Critical MmD Mean N-Mean D-Controi 33.700 1.0000 33.700 29.000 36.000 6.105 10 35.133 1.0000 10.98 32.900 0.9763 32.900 30.000 36.000 7.228 10 0.644 2.287 2.842 35.133 1.0000 22 34.700 1.0297 34.700 30.000 39.000 8.914 10 -0.804 2.287 2.842 35.133 1.0000 43.9 35.700 l.OS93 35.700 32.000 41.000 8.563 10 -1.609 2.287 2.842 35.133 1.0000 72 35.200 1.0445 35.200 32D000 40.000 7.311 10 -1.207 2.287 2.842 35.133 1.0000 100 38.600 1.1454 38.600 33.000 43.000 8.566 10 -3.942 2.287 2.842 35.133 1.0000 AvnlIry Tests Statistic Criticl Skew KIrt KoMhgmvD Tes indicates norm, distrlbunon (p > 0.01) 0.73571324 1.035 -0.0294286.0.8460054 Bartlett's Tes indites eqmd variances (p - 0.74) 2.73092866 15.0862722 HNOhe-s Test (1-tal, O.05 NOW hVDEC CllV T MSDt MSIp M8B MSE F-Prob df Dunnetits Tes 100 >100 1 2.84245189 0.08434575 39.1466667 7.72592593 7.0E-04 5,54 Treatmets va DwControl sqn_07-07-04data

TVA / Sequoyah Nuclear Plant - Outfall 101 Non-treated Statistical Analyses U bzwphThx (2Op Remd_) MMw R FD 9% CL i005 r0 XRO laO X=# m En0 >.100 I >100 >100 >100 O.L 0.8 0.3 0.1 0.0 .0.1-2A - .0,0 b .t 0 20 40 0o so 100 120 Dome % -R_ nut 45. 40 35. g 30. a820 1 5 Is 10 S 0o I-= I 14.1,0.05 Ive ofewfldm I d i '.4 S.- qn_p7-O7-X4data

TVA / Sequoyah Nuclear Plant - Intake Non-treated Statistical Analyses Cnlodsphnia Survival mnd Reprodyetion Te t--Reprodve~ Stat Date: 7t7/2004 Ted D.l CdMRCR Sanupbl D: A I Sequsy Nucler Plant - Intake End Date: 7/142004 Lab MD IB-Envr. Testing SoL Savle Type: DMR-Disohgp Monitong Report Sa&mV Date: Protol: CHRON I-EPA-821-R-02.013 Test Species CD-Ceriodaplnia dubia Conments. NON-TIhAThD Cone-% 1 2 3 4 5 6 7 8 9 10 D><antrol 36.000 35.000 36.000 30.000 34.000 34.000 29.000 35.000 34.000 34.000 100 39.000 44.000 33.000 40.000 37.000 33.000 42.000 39.000 36.000 41.000 Transfom: -Untransformed Cone-% Mean 1-Mean Man Min Max CV% N 1-Tolled t-Stat criticl MIM Isntonl-Mean N M. D-Cbntrol 33.700 1.0000 33.700 29.000 36.000 7.001 10 36.050 1.0000 100 38.400 1.1395 38.400 33.000 44.000 9.525 10 -3.415 1.734 2.387 36.050 1.0000 Avm!r Tesht statismf CQicel Skew Kurt Shapiro-Wik Teot idicates nmnml distribution (p > 0.01) 0.93153244 0.868 -0.4628473 -0.2391617 F-Teo hyficates equal vinces (p 0.21) 2.40319371 6.54108953 Hypothesis Test 0.0) MSfu MSB MS -F-Prb df Homooedastic t Tesd indicates no significant differences 2.38674469 0.07082328 110.45 9.47222222 0.00308902 1, 18 Treatments vs D-Control sqnO7-07-O4data

TVA / Sequoyah Nuclear Plant - Intake Non-treated Statistcal Analyes obw PrM RD n% CL ICs l106 T2S Tr >100 >100 >100 >100 >1o0 >100

I.g I 0.9 0.8 A.7

[0.6 a OA* 0.2 Q.1-nnl -Q1* .0Z r. 0 20 40 60 so 100 120 DM* Dmus-Rexp~Met qj j ff 40 35 030~ 16 10 5. I......................................................................I I 14b.60.05 kem Ofstnleance WI.I sqn O7O07-04data

Envlronmental Testing Solutions. Inc. Page I of 6 Fnvironmental Testin! Solutions. Inc. Page bE 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA Facility: Seguovah Nuclear Plant - UV-treated NPDES #: TN 0026450 Project#: 13?ZI Dfludon preparation Information: Comments:' Dilution pe (%) l 10.9S l 22 1 43.9 721 100 r-tAl 5 4AUP -A&fiArrOTJAJ1JAPS45 Effihent vWlume (mL) 274.5 f 550 1097.5 1800 250 IT L 2A9tu IDluevlz (iL) 2225.5 1950 1402.5 70 0 tn O Q A l C Total volume (mL) 2500 2500 2500 2500 200 rreA e e s, Test organIsm information: Test lnformation. l Organism age: 21S.fl rs mo Randomizing template: R Date and times organisms C-o6-tq %bctl %S 4tT Incubator number: were born between: Organism source: AS_ 6 IS 61-M'4* Anrtemia lot number. BberlS5 Transfer bowl information: pH Temperature - 'c Total drying time: 1.Ss Zq.S Date/Time in: av-i4 I Average transfer volume: Date / Time out al 1fl ait 0 v Oven temperature: V'C Dailyfeeding and renewal Information: Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time I 0 *O 0&'Sb _lSC> 113SOM 01-%O>LbJ 04CU.04,3; o2 15 s6 O1-btoW0 4 0 00, I 3 101.1o ONI AS6 I -a&3 tn.A-Oq Oq 01'-.-02-I1 .07 At

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Dn. 110 _q_ O

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07-otc4 4O o.o 1.I6 65c

07.

is rss4 IS ____6 e Io. 0 0-4 B. O&$ t6 I's5 -Zs 144M 10n 10 *el/Ai KEl 7 __I;I2v .X :'3t I., A -I-Control Information: l Accptncenti Summaryof testendpoints: % Mortai Z.S7 l s20% V 7-day LCm _I Average weight per initial larvae:

6. 0S5 3

NOEC Average weight Per surviving larvae: 0.112: 2 0.25 LOEC ) I mfl ChV IC25 -- )I 7.

Environmental TestinL Solutions. Inc. Page 2 of 6 Environmental Testln Solutions. Inc. Paze2of6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: 01-04C SurvI 0and GrowthData _ Day CONTROL 10.98% 22% A B C D E F G H I J K L 0 /0 /0 /o1o1 10 10,0 10 to to /0 /0 /C 1 ) _ 10/0 - to -/ /O 2 IC C > lo CIL) 10 lb I 0 /O /0 i) to /D/ /0 /t /0 to /0/0 /0 /t /0 /0 /0/0/0 10 / 0 _ /C)/0 -/ 10 / to s_0 g) 10 V 10 /d d0 /6 6 -0 -t °-/ °/ t t{)1 lo______ lo / l o lo010/0 /0 /0 /0/to 10 _1_ IL) 9 q o

0 JO toIO 10 10 10 A-Pan weight (mg)

B - Pan + Larvae weight Lare weight (mg) - A - B 2 1 pa s e<: ,¢ * \\' Weight per iultal number or A 4 - Cl Inltal numberoflarvae O b 0-O- 6 Average weight per Initial number oflarvae (mg)

0.

0. L, 0.101 Calculations and data iewed:4 Comments:

Environmental Testing Solutions. Inc. Page 3 of 6 Environmental Testin! Solutions, Inc. Page 3 of 6 Species: Pimephaespromdas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: o01-01oct SurlsvaI and Growth Data Day 43 % 72% l-1% MN 0P R S T U 0 /0 lo 10 l0o / to 10 1o /o 10 10 /O I__ ID /O /O o rIC)to O oto /0/10 to 2 I'D lbD 10 /t la lI /D -0//6/C) /O 3 /0 D /O O O / tot /6 10 4___ //0 / /0 /0/0/O _0 _0 / O / 0 10o la0 Idoq 1') /O-° 1 o lo 6 I / /6 100 to 6 to / 1./0 _c1 IL_ ) lo lo0) J lo io 0J A/O A - Pan weight (mg) B - Pan + Larae weight _V~ _~ (Rig)tperlultlalaumnberef A a aJA. Larnce weight (md ) A - B +U'% o tw> /5>5 n 8 .CIIltfal number of rae Q, O O O' C) O Average weight per initial number of lme (mug) O.Ls alculations and data reviewed: Comments:

,f I....Environmental Testing Solutions, Inc. Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - U-treated Date: C -O'?-c Survival and Growtk Data Day I-100% Intake Y Z AA BB 0 10 10 /0 /0 o( tob t0 / S0 (_ /6 / 6 IC )

R 10 7_

_l a 1 0 g0& / A - Pan weigbt (mg) B -rPan + Larvae weight Larvaewelgbt((mg)-A-B B U.__ 4,. Weight per Initial number of A lame (mig) e - C I nitial number oflamse 'Q Average weight per Iltial number of larve (ng)

0. 6O6tv Calculations and data reviewed:

'Comments.-

TVA I Sequoyah Nuclear Plant - Outfall 101 U -treated Chronic Whwe Effluent Tor"ity Test (EFA-U21-R013, Method 1000) Speder-Pmephakuspremda Quality Control Vedtfleatlo f Data Entry, Calculations, and Statistkal Analye JAIlqm rstVf Te aft m.1 aty h I 2 2006A Rm I " " b r _-Am k 4 9 ^- -tL-- -S-"o pns -- U

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acb.) smmbs Twbkt~wd ind (V.) A 30 10 15.04 275 7.71 0.771 o. a 10 10 14.73 21.01 6.23 0.6207 3 5 IH 3e C to 9 35.00 21.07 0.61 "4 0.607 10.51* 04apa3 D 10 10 14.5 22.33 7.75 0.775 0.775 E to 10 10

34.

21.40 664 0O44 0.A44 F 10 t0 15.01 22.02 701 0.701 Q701 to t0 14.70 21.65 695 0.65 0.01 1A" s s n. 10 le 14JS 20.6 S.06 _OA6 Oe60 I 1 15.07 2L46 7.39 Q739 o0.739 J2 o 10 30 15.02 -22.30 7.23 0.7213 0.707 GA 0.723 O .07G L K 10 10 14s4 21.24 6.0 0640 1 07 66 -L7 L t0 10 SAS 7t L_0 t22 0Q722 Q M 10 10 15.16

23. 0 7J4 0.747 74 10 30 15.02 2L.

6.67 tAll A6m7 0 10 14A2 3.03 6A= 0.661 t0.61 r 10 10 14.64 2IA9 6.5 0.635 0.635 = 10 10 15.00 21.41 64 0.41 0.641 72% R 30 o9 1497 21.39 62 0.713 Gm

4.

0642 97 6A67*

4.

4.1 5 toto -4.7 21.7 702 07020.702 T 10 10 14.70 21.52 6J2 Q652 0.62 U 10 30 14.60 22.5 791 0.791 0.791 10% v 10 30 15.33 23.29 0.796 0.755 6.0 0.796 133

40.

Le 4.6 W 10 30 14.72 22.0 .2 0.723 0.728 X 0 10 14.74 210 0706 0.706 t o lo 14.43 213 67 0fi7 efi7 IOM z 30 10 14391 22.35 7.24 0.724 0.10.724 .63657 AA__ 33 30 14.7 23.32 .56 0I65O 0_91 0 0.656 "A S 1-BB to It 14.76 21.32 6 X OAX oAl6 Qah" 1M1 DemeiaSDwIme: webs howmgno pMSDO 11.694 126 U.122 36.3 POD-Mh ~~iSDIlec FM.D ?eft bfibsma~dmluetDiffeuw awea_,lmld_ dtfieum hcawrciiaTua Sand Iuebee..hddyeeuhatocawuupsAu awnb 34.% Doe eI(dei..dae LowerPMSDbo_ _wAee

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-9 Uppr9UDi dpe00 tUMEpA(0&V-snfl)-35S% albee uduyapusa s we eale4 bWy the USeP les k 205 Add _l u ml ow meke bWhetucief USWA. 200 aunt m Ao t Mei Vo Wole E Ap U u Do E EPA.334.003. US w a o Alm A hed. 01.

TVA / Sequoyah Nuclear Plant - Outfall 101 UV-treated Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Stat Date: 772004 Test 11 PpFRCR Smple Dt TVA / Seqioyh Nuclear Plant - Outfall 101 End Date: 7/14/2004 Lab mD: ET-nvir. Testing Sol SMplo Type: DMR-Dirge Monitorg R rt Sample Dete: Piutool: CHRONIC-EPA-821-R-02-013 Test Species: PP4' aes pmelas Cm

miat WV-TEATD.

Come-1 2 3 4 D-Contral 0.7710 0.6280 0.6070 0.7750 10.98 0.6440 0.7010 0.6950 0.6060 22 0.7390 0.7280 0.6400 0.7220 43.9 0.7840 0.6670 0,6610 0.6850 72 0.6410 0.6420 0.7020 0.6820 100 0.7910 0.7960 0.7280 0.7060 Transform: Untransformed COW% Mean N-Mean Mean Mh Max CV% N D-Contral 0.6953 1.0000 0.6953 0.6070 0.7750 12.974 4 1O.98 0.6615 0.9515 0.6615 0.6060 0.7010 6.799 4 22 0.7073 1.0173 0.7073 0.6400 0.7390 6.417 4 43.9 0.6993 1.0058 0.6993 0.6610 0.7840 8.211 4 72 0.6668 0.9590 0.6668 0.6410 0.7020 4.542 4 100 0.7553 1.0863 0.7553 0.7060 0.7960 5.974 4 I-TO t-Stat CrM4 0.860 -0.306 .0.102 0.726 -1.529 ifed Iotolc icad MSD Mean N-MeOn 0.6975 1.0000 2.410 0.0945 0.6975 1.0000 2.410 0.0945. 0.6975 1.0000 2.410 0.0945 0.6975 1.0000 2.410 0.0945 0.6975 1.0000 2.410 0.0945 0.6975 1.0000 Auli Test statistic Critieal Skew Kurt Shepiro-Wilkb Te indiates noml distibtion (p > 0.01) 0.95757478 0.884 0.0475234 .0.9287226 BartlettI Teat indicates equal vrianc (p = 0.59) 3.73211074 15.0862722 HpThesis Test (1-tal, O.O) NORC LOEC ChV TU MS~u MSDp MSB MSI F-Prob df Dumnett's Test 100 >100 1 0.09454345 0.13598483 0.00454384 0.00307793 0.2462927 5, 18 Treatmnts " DiControl sqn_07-07-O4data

TVA / Sequoyah Nuclear Plant - Outfall 101 UV-treated Statistical Analyses xJX,,w-bXeem(2"0 Resuiyhs) 1 an 593% CAEx1) -s ICIO IC'S id20 RU25 IC30 nco >100 >100 >100 >100 >100 >100 >100 -i.0. a... 0.7 as Q5 ! 0.4 ' Q46 0.2 0.1 ULU - e e -Qt 0 20 40 80 80 100 Dote % 120 Imt-R-pm 0.9 - 0.7 p?0.4, 0 Q.2 0.3 1-ut 0.0S ofugimlN n. 2 sqn07.07-OMdata

TVA / Sequoyah Nuclear Plant - Intake UV-treated Statistical Analyses Larval Fsh Grwth and Survvl Tet-7 Da Growth Stal D&te 7/7/2004 Test lD PpFRCR Smle ID, TVA / Sequph Nuclear Plant - Intake End Dat: 7/4004 Lab ID ETSnvir. Testing SoL. Sanle Type: DMR-Di shap Monitodng Repod Swnph Date: PmooL CHRONC-EPA-821-R.0o2013 Teat Spesx PP-Pimophales pomelas CommentE V-TPATED CGn 1 2 3 4 D4Contol 0.7710 0.62R0 0.6070 0.7750 100 0.6870 0.7240 0.5560 0.6560 Transform: Untransformed ConM-% Mean N-Mean Mean Mil Mal CV% N DwContn 0.6953 1.0000 0.6953 0.6070 0.7750 12.974 4 100 0.6558 0.9432 0.6558 0.5560 0.7240 10.991 4 1-Tailed Isetnoc t-Stat Citical MSD Mean N-Mean 0.6953 1.0000 0.684 1.943 0.1122 0.6558 0.9432 Aurdiary Teds Statistie Citical Skew Kurt Shapiro-lVfk Tet idcates nmrn diftribution (p > 0.01) 0.86392242 0.749 40.3059472 -1.9996062 F-Tes incates eqmul vmices (p - 0.72) 1.56619453 47.4672279 Hypothesis Test O1.Msl 05)MSfu MSIpp MSBI MSE F-Prob df Honwaoedastio t Tet indicates no significant differences. 0.11218045 0.16135268 0.0031205 0.00666558 0.51937759 1,6 Treatents vs D.CcotnW sqnO7-07-04data

TVA / Sequoyah Nuclear Plant - Intake UV-treated Statistical Analyses MWd RHe ridt mD 9% CIAM"ie 1D5* IC10 r:20 =0 rAO W!o MON >100 >100 >100 >100 >100 >100 in -.

oivtl B fin lk s cO.

lowes 0@ Usc G us 0.9' 0.7 E QE 0.6-S n 0.3-OL2-M.1 nn 0 20 40 60 O0 100 120 Dose % U.9. 0.9 0.7 80.6 tQ4 0.2 01 of s8gnifican n.Ii sqn_07-07.04datb

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxdcty Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Daily Chemical Analyses TVA/Se hNwdewrbd, Not' ed Test dr. Jly 97 14 20 P 3ectnmwer 1321 I d6 .1 k ToI a To.UL-I TL_. I F-cefteo ign C n UIS C-DO (Mi/) 4-COuN&WTjfty (H~nbsdfln) cam" 'U-em-ong IC& A v 4-S rffinewaeMs enx 4.777 4- I---$ -t -t !I !t on min

N, -- -,,

nDb il) 10.8% rfa.. u f ( - l T C) 25.2 249 25.11 24.81 25.21 24.5 25.2 243 25.41 2421 25.11 24.41 24.5 24.1 wH 7.64 72 758 7.81 8.04 7.81 8.16 7.74 8.02 8.06 8.16 8.02 1.22 7.81 22 = 7.8 7.5 7.6 731 7.61 6.9 7.8 6.7 8.0 7.91 7.61 8.1 7.5 22% C ( _) 246 254 228 280 274 m 252 247 25.1 24.7 253 24.5 25.1 24.1 252

24.

25.1

24.

24.5 24.1 v1_ 7.63 7.7 7.55 7.8 8.03 7.85 8.15 7.80 7.96 8.0 8.15 8 8.19 7.79 D ni+L) 7.8 7A 7.6 7 7.6 6.9 7.9 6.8 79 .7 8.0 7 82 73 4&9* 2~ 381 24 2 - -24z5a Z43 261 255 25.0 24.7 25.0 24.7 253 242 253 242 25.2 2.2 253 24s 244 243 7.60 732 7.54 75 8.02 7.86 8.16 7.83 7.91 8.14 8.04 8.20 7.82 7.8 7.5 7.6

72.

7.7

6.

7.9 68 -8.0 8.1 7.6 8.2 7.4 729 i t ~*t~l6"nwd~n) -214 2Z0 2200 22 220 236 233S T C) 25.1 24.6 25.2 24.8 25.4 ?4. 25.1 242 25.2 24 25.1 24 24.4 24.0 7.591 7.33 7.49 7.90 7.99 7.96 8.14 8.01 7.84 8.16 1.13 8.12 8.18 7.9 7DF.L) 7.8 7.5 7.6 7.2 7.6 7.0 8.0 7.4 7.8 7.8 8.1 7.7 82 73 9=kn =!fLM~es d19 -191120 19' 5 2r 100 OBLCKW 781 g TOW ReddNl aCwhsne O"A.1) TIpewrprnC) 25.0 24.6 252

24. 8 25.5l 24.5 2

25.1 24.0 25.1 243 243 2 PR (U) 7.671 7331 7SS551 t 8.121-793.0 .91 .541 8.171 8.361 8.141 8.471 .6 DO t ) 7.91 7.51 77 7.81 7.01 82 8.71 7.91 S.11 7.71 8.3 7 gn+/-1563t !!2_ 197 i 19415 0 100% bJune Afkfbtty (ni/L COC0) 79 81 R__ _On 9t0L 90 9R2 1 Tea ReuInduae Q </L) O. Te-pwtms (C) 2531 24.91 25.0 24.7 25.21 24.51 25225.1 2431 25.2 2431 24.71 24.1

I ILI. Environmc I Spec Daff I= Conce I coi I .ntal Testing Solutlons, Inc. Page 5 of 6 les: Pimepholspromelas it: TVA / Sequoyah Nuclear Plant - Non-treated Chaemst: Date: 6 O1.oa I ICAwFI4P^s~ I Day I I 0 I 1 I 2 I alyst ertrtioo IParameter FTROLI pH (S.U.) _~~~ I.r DO (m/L) r Conductivity (umhos/cim) ,=_ _ Alkalinity (ma CaCOL) z v - ir - Hardness (me CaCO,/L) x--D Z _E Temperature

4.

pH (S.U.) 10.98% DO (mg/L) Conductivity tuFmhos/cm1 Temperature (C pH (S.U.) 22% DO (mn/L) Conductivity flmh/em Temoerature (°C) pH (S.U.) 43.9% DO (mgtL) Conductivity (pnhos/cm) Temperature (C) oH (S.U.1 72% DO (mmIL) T. B Conductivity (Amhoslem) I L1q Temerature (C) 4 .9' ---- pH (S.U.) .51 s I 100% DO (mg1L) Conductivity 2 (Amhoslcm) Alkalinity 7¢ (mg Ca4COYL) Hardness e (mg CaCO3/L) TR chlorine (mg/L1 0.10 _w. 2S0 1-I \\ L 2S.t 2A.L6 ZS- 'fs l 214 ZS. 3 'Z 6.S t.cz -q 14.2 7.6 2A m 24.-4 _5.3 If4 8.tY I Temnerature I'C) 75.0 ,nT {s ii V: 17 fl I I 5 100% Intake DO (mOIL) if.). lef Conductivity .(ga1 .(wnhcm) 1 I t 1. Alkalinity 4 (mg CaC03/L) Hardness O (mg C8CO3 /L) 0 TR chlorine ZD. ISk (Mg__ 1 = F4 .4-4 Zi2 Temnersture (°C) I. Z5*U 9:

m.

Intial i-. Initial I -Fina-Initial I Floal - h

r"_T_7- '11, 7 I Environmental Tesdng Solutions, Inc. Page 6 of 6 Species: Pimephakspromekzs Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: la-.C4 14 I I Day I I 3 I 4 I S 4I --, r Concen-Parameter traton 'I CONTROL 1pH (SMU) DO (mg/L) Conductivity (umhos/cml \\ I Alkalinity (mr CaCOIL) 4 I Hardness 1wc C&COJL) t~~ Teanerature (MC) I II OH (S.U.) 10.98% DO (mgWLU Conductivity (umhos/cm) Temperature (MC) pH (S.U.) 22% DO (ml/L) Conductivity (uimhasdm) Temperature (C pH MS.UM. 43.9% DO (mk/L) Conductivity formhtm~rm) Temperature .U IC t1f 72% DO (mg/) I e Conductivity (pimhos/cm) I I 1 8.l ,*.Ii -~ -2 3 M-Temnerature ('Cl ZT2s.7 7S.1 2'. a Zq.O I-- pH (S.U.) (A.14 100% DO L(n^)j l 6.0 Conductivity (pmhoscm) ZOO Alkalinity (mg COCO3AL) Hardness TR Oiorlone (nmgIL) Temnerature pH (S.U.) 3.ZO 100% DO (MgL) 8.4. Intake O_ Conductivity qq (pwcyn)I t Alkalinity (mg CaCOL) Hardness (mgCaCO 1/L) TR chlorine (fmgL) Temperature ("C) IS.. Initial I

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toidelty Test (EPA-821-R-02.013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses cmlent TVA /SeqOe IN Tat datw. Jl 07 -14, 204 hPchT ]M, Nontrmfted "jetW vmba 1321 Rerk t rdyby-9fi&2!.* -I csaCtsom I t. D ayl I Day 2 l D "3 l DRy4 1 41 741 8.09 8.091 8.12 8.21 8.23 8.15 76 7.8 7.6 7.8 7.7 7.8 7.9 7.7 7.8 11I IstI DO (mi/L) 7-9Tfndutmtvftim Wa-rM) Albifty (*WL CRCO,) fflsn(mwLCwC03) I C)e C 25.4 24.9 25.1 24.8 25.2 24.9 25.2 24.7 24.9 24.6 2.0 24.7 245 25.2 pH (SU) 7.65 7.46 7.581 8.06 8.0 8.11 8.15 8.21 8.08 8.16 8.17 8.24 8.23 8.04 M1 DO hML) 7.7 7.5 7.6 7.6 7.6 7.8 7.9 7.9 8.1 8.1 7.9 Cebdwedy" 2U 261 266 28E 268 287 2 8 1 ~ _repe C) 25.2 24.9 25.1 24.8 25.2 24.9 25.0 24.7 24.8 4.6 24.9 24.7 24.4 25.1 pH(SU) 7.64 7.47 7.58 8.07 8.04 8.12 8.16 8.2 8.02 8.16 8.16 8.27 8.22 8.05 Do (DO L L) 7.8 75 7.6 7.7 7.6 .7 f 8

7.

8.0 7.8 7.9 8.1 8.1 7.8 22% _m) 246 25 261 26E 5E 280 274 Temp e ( 25.2 24.9 25.0 24 25.2 24.9 255.0 24.71 24.8 24.7 24.7 24.5 253 Oa11) 7.63 7.47 7.55

8.

08.0 8.13 8.15 8.22 7.96 8.19 8.15

8.

8.19 8.0 DO (mg/L)7. J 7.8 7.' 7.6 7.6 8 7.9 8.0 7.9 8 .28 43%238 _2; _244 250 243 261 _25 T% -h"e C 25.0 24.9 25.0 24.9 25.3 24.8 25.1 24.7 24.9 4.7 24.7 24.4 252 I _(__ 7.60 7.54 8.10 8.02 8.19 8.16 8.27 7.91 8.23 8.14 8132 8.2012 72% Oi Lu) 7.8 7.5 7.6 7 7.7 7.8 7.9 8.0

8.

7.8 8.1 8.2 7.8 ondu""I b ) 214_22(0 221 221 220 236 _233 CC) 24.9 25.0 25.2 2 25.3 24.8 25.1 24.5 25.1 24.6 24.7 24.6 25.3 H(SU) 7.59 7.51 7.49 8.1 7.99 8.23 8.14 8.29 7.8

8.

8.13 834 8.18 8.14 DO (mdL) 7.81 7.5

7.

6 7.6 8.01 8.1 7.8 7.8 8.1 8.21 7.9 . 1I~tmmLm 204 _12_1 0 9 1 0 100% ALkal CRt) 77 71 77 dn (Mg/L CC) 388 8E 90l T&t RaldeaChline (n wL) 0 0 cO.10 La"____ != CC 25.01 24.81 24.91 24.81 25A 24.9 253

4.

2.14. 2.447 2.753 pRl(SU) __ DO SML 7.67 7.4 7.551 8.13 8.121 82.0 82 85.14 824 84.31 84.33 84.471 2.11 Do('L) 7.91 7.51 7.71 7.6 7.8 8.01 8.2 7.8 8.7 7.71 8.1 8.11 8.3 7.9 100% Intel Alality (n/L CpCO,) 79 8 flrdM (M9LC" 3 90902 Total Refdm rle (wiL) T"peatur _c) 25.31 24.81 24.9 24.9 25.01 24.8 Z5.1 24.7 25.0. 246 24.6 24.7 25.0 253

Environmental Testing Solutions. Inc. Page 6 of 7 Environmental Testin! Solutions. Inc. Page 6 of? Species: Ceriodaphnaa dubta Client: Seauovah Nuclear Plant - Non-treated DaftA ChenLtry: Date: O4 ' °. Co d t f A "aROcs) i~~0 (eese o fe-Day I _-= 2 Analyst bs I 7c8 IYCD IY' B I t.6 U Concentration Parameter CONTROL OH (S.U.) 1A5 &vd ?A11 .1 1 ILM I $. I Ai 4 2 I DO (mgVL) ,I.., I Conductivity Z (gmhos/cm) _3 Alkalinity (mg CaOO3/L) (el Hardness (mgCaC03/L) I If \\ N. - _2s.t1 _ -M I 6.d Temnerature IC pH (S.U.) .65 10.98% DO (nmAL) -I Conductivity (nmhos/cm) Temperature("C) 2Z pH (S.U.)

7. EA 22%

DO (mg/L) S Conductivity (Irnmhos/cm) Temperature1°C) 25. _.6 7.6 7.6* i 26 e* 8.0 1 2S. I z.BZ 5Zs.Z = w.s M &V 0.04 7.6 Z 94 2S.O .6 _ zz I I I Zy4 pH (S.U.) ?. 6E = I.sST j ECs 1 GV B.1 43.9% DO (mWL) Conductivity 1 39 2s0 (;Lmho I MN ZiI.6 iI 2Y. Bl91 Temperature MC) 7.s. 0 a - -b. pH (S.U.) . 4o 7.}s 72% DO (mg/L) 7. Conductivity 2*1 (Pimhos/r) 2 Temperature (QC) 2qw. 'S 9 pH (S.U.) I.51 4 - 100% DO (mglL) 4 Conductivity Zoq (Pmbos/cm) Alkalinity (mg CaCO3lL) _7___ Hardness 6 (mg C8C03/L) TR chlorine _mAL .7 Is wZZ Z53 = W~t I V.,6 M Jq '1 45 1 85 I 40.10 _ Asn 2.8 Temperature (°C) ZS. U pH (S.U.) I. Vf 100% Intake DO (mgfL) ).t Conductivity Alkalinity. (mR CaCO/L) 9 Hardness q g (mg CaCO1/LP TR chlorine -I F a Temperature (C) I 3I S.Iz 14.4! I i i initialfin Initial I I

Environmental Testins! Solutions. Inc. Page 7 of 7 Eniomna etn!Sltos n.Pg of 7 Species: Ccrlodaphnia dubla Client: Seauovah Nuclear Plant - Non-treated Date: WI.DJ1.-b UI Day I II .3 I 4 I Analyst_ Concen-Parameter e tntlon 1I CONTROL pH (S.U.) DO (mgIL) Conductivity (p~nhos/cm)_ Alkalinity (mg CaCO3/L) Hardness (mg CaODVL) Temperature (MC) 10.98% DO (mA/) I 6 -V - +q9 Conductivity Iv 9 ITemperature M') 25.0 9 Ii

1,W Ip ie

\\2. 6a.1 Ze. C B7P PH (S.U.) e-.to A It .3-22% DO (mg/L) 8.1 142 Conductivity zco -M a i I Zi14 Temperature (C) -2.S.0 ZWX pH (S.U.) B is a 43.9% DO (mg/L) .9 Conductivity mm (pLmhos/cmn)v Temperature ('C). pH (S.U.) ex" 1 .2 q i 72% DO(mg/L) W .E O 6On Conductivity Temperature 'C) 2JS. pH (S.U.) 100% DO (mg/L) Coductivity (iunhos/mi) Zoo Alkalinity (tgCBC03/L) Hardness \\ 4 (mg CBC03/L) TR Chlorine (mgL) Temperature('C) .-3 VJ 1 Z 3 pH (S.U.) ism 100% DO (mAL) intake Conductivity (ymhocm) IqI9 Li Alkalinity (mg CaCO3BL) Hardness TR cklorine (mglL) .10O Temperature (C) _ZS. 7 Z'.4. 19 f l. 3q fi l g 1.1 I R.-I -i a als FM= Nf-ZT. L_ 7'I4.1 ,-5-u I Zb.. I F11--i InItW us, 1nitial Msa1 Initlal Iia Initial Final a

Environmental Testing Solutions, Inc. Chronic Whole Eluent Toxicity Test (1PA-821-R-02-013, Method 1000.0) Species: Pimephalespromelas Daily Chemical Analyses CWLe TVA Tatidaft: July NoPe mmike 1321 I ShN"ader t, UVN.kTd 07-14.24 I DV Fn I D" 1IF I jd"a. II. '.55 7.28 7.441 7.78 8.091 7.77 7.81 7.5 7.6 7A 7.7 7.01 ,H (iSL L DO (mrlL) C-fl-e~ Arb hMLNw wS [frdRenXCA.% 25.21 25.11 25.01 25.01 2531 24A1 2531 24.2 25.41 24.2 25.11 24. 24.51 24.2 7 .q1 -1 d 7 7 .0.1 7 a..- taA. A a tap 7i1 S1. I, ,H (HU) l141 ,1,. 11 .4jI 1.1l 1-01 1.101 .YOI I.11 f.I o.wI o.v: D.&ol I."F Ob.,1 1.01 10.% I 7.8! 731 7.61 721 7.71 7.91 7.81 6.91 7.71 8.01 8.11 72 8.41 7.2 = 23 260 270 277273 291 28 283 r_ do^ 25.4 Al 5.q11 27.11 25.1-25.11 24.2 25.21 24.2 25.01 24A 24.61 24.' l fnn nut % -71 7 7 7.4 .2 741.5 vn0 £u.fl. R.0 7.3 7.6 7.3 22% t-iini S ,,,_.^_\\ iwu~y~ liuru

~

-. 5 I I Trm.4me 1f* 2S.41 25.01 25.11 25.1 pH (SU) 7.531 733 7.441 7.85 4DO nL) 7.8 7 7.7 7.3 c4mdwlm (% l) 254j_--I:25.01 237 Temp"n' CD __ 253 25.2 25.0 1 df i7 7d 8.111 7.76 8.031 8.06 8.16 8.0 8.24 7.79 7.8 6.7 7.8 8.0 8.0 7.7 8.4 72 269 261 283 279 25.! 24.! 25.2 24 25.1

24.

24.6 24.3 8.10 7.76 8.02 8.07 8.17 8.0 8.27 7 7.8 6.7 7.8 80 8.1

7.

8A 72 249 2*4 265 211 25.2 241 25.2 24.0 25.1 24.' 24.5 24A 8.05 7.95 7.99 8.14 8.18 8.08 8.25 7.78 7.9 6.9 7.7 8.0 8.1 7.6 8.4 772 2220 23 235 "H ENI 1 -1.3 1 J 1.1* ."I ,.71 72% DO (m74L)- 7.7 7.'1 codfu~y (nbem)h 228 2138 ITeffmverture -Or ^ l ..I Wil 2i.1 25.2 7.3 7.44 7.6 7.7 25.0 7.88 2SA1 24.1 8.07 7.91 7.94 8.13 8.19 8.09 25.01 24.2 25.21 24A 24.3 7.89 1J ,..MI iO Of M7f a I 100% A02fl (I) CC T9RedwCb] ln(mL) DO (su/) I hdkifit aceC)- 100% Wk

Solutions. Inc. Page S of 6. Xc-ei-^Ziiiephdispromelas ientr TYS I Sequoyah Nuclear Plant - Vtreated Date: C R - 07f

o Aey f A4, fai\\ ewe

. I w \\ (exceed A" {q And I Day I I 0 1 11 --x -11 as n own oal 0A A l Analyst I Parameter Ala w w-^ s E

,^->

-i!-'; St.'"",',e.s, A,, An-.'.,% 1.,, An;-', I':', act: *; .tt.:. ' - At '_'-i-t,. JW '}ids f A.,.{-{. ,$.s,:

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bp at An' J.i i" IA f i-. :, .K, tl I r pH (S.U.) DO (mglL) Conduletivlty htnnhnndem%

r. __

--A And He l M= zipS 1 w. AHainets m lG R S \\; Hardnessn l, E er0s -S& f I WJ-la I a I i.}(S.U.) If5 AO 14AS F.76 qn.7e

r;E.,

A-'S',Couductiviqty ~ ~ Temperatore (°C) 2SA t t5. 0 S 25-

g. 1 Z45 3 ZA.7-O.

H (SMU) ML 5,} ~Q .s A -22%139e DO (mgL 7.> 7 477. .t t ~Conductivlty 5 g2 B

_Tempereiurt (OC)

Z5A 2S S 3 Z5 0 Z, 2. 7 ~~~pH (S.U.) 7}5 .jy X,5 ,sz 6 X R~ 3.972 DO (mg/L) 7S t77.'s t o1 ~~~Conductivity2 3 l 0 .Temper ture (eC) 2,351SZs.O Z5. 2 Zl* pH(.U)TA .4 A 5$6 I OR (S.U.) Y. $7.9 I sq ISO% DO0(mgAl) Conductivity (Ndlos/cm) A.lkalinity (mg CaC03/L) Hardness (mg CaCO3/L) TR chlorine (mg/L) Temperature (°C) _lll _ § l nN MMu. 100% inakie rig---- I {x,.. l S DO (mg/L) Conductivity (mg CRC0O/L) 3 Hardness (mg CBCOVfL) TR chlorine (mg/L) Tempver ture (°C)

Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephakspromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: nZn4b4 qI Day I. I I 4. Analyst I Concen-Parameter tratlonI CONTROL 81H (S.U.) \\_._ DO (mg/L) \\_ Conductivity (pmhos/cm) Alkalinity (mg CaCOIL) Hardness (me CaCOi1L) Temperature (C)

6. -

OR (S.U.1 ,_~~4 10.98% DO (mglL) Conductivity I (pmoscm 1Z-44 IM Z4I I aZ4 I 1o 7-Is Z86 M v~ Temnerature ("C) ZS.A 7ZI.7-2S.7 -(S.U.) I.16 22% DO (mgtL)I = Conductivity 7I.O Zw I Temperature 7C' X. \\ .'l. Z.S-2.. n 7.65 ME Z'7'q 1 2_5. % I 1.4 I 'Ld I( 21.(o z H (sMU.) v*- w 43.9% no (mOL) Conductivity I (plimho s /ci m) I2 Temnerature (C) pH (S.U.) 4.Dd4 72% DO (mg1L) i Conductivity I H (eemhostcm) I"3 Lemperature (°C)

25. 1 L

_ a0om 1.0 S. 1'7 -4. qq 8,Q8.16 -Z2z 2 Zs I

5 - 6 zZ 28-oz I *

= 6.z-1.4I = 6.4 J. P L 2.5. zY.4 =8.z Z.7 Z1a55 _ 8Z5 7T-M A.L 4

14 011 (S.U)

\\_._., 100% DO (mglL) \\ Conductivity (pmhonMcm Alkalinity (mgCaC Hardness (mg CaCO/L) I TRChlorine m i Temperature (C) 100% intake pR (S.U.) I 0l5 DO (mV7g) . Z 4.1 W0 Z C.a. cF.cI

a. C-I kw-C k645 Q9.2fz Ws 6.1B.

f I .0to

1.0 Be 8z' I

l_ 00o 21i 15 8.4-- Conductivity mhoslcm) Alkalinity (mg CaCO3/L) Hardness (mg CaCOVL) TR chlorine ni Temperature (C)_ I I Il I 'ZS I Final I Initial

I I I I I I Page 41 Pate I of Z Environmental Testinn Solutions. Inc. Alkalinity (EPA Method 310)1) Matrix: Water, MDL -1.0 mg CaCO3/L Analyst [ 57 ] Date analyzed _ O1 A I Titrate samples to pH = 4.50 S.U. Titant no and mdtiplier deetrmiadon: pH of Normality Normality (AM of H2SO4 pH Factor or Multiplier Delonized Titrant check Begin End Total (5 ml Na 3CO3 x O .O5)E (Ni 50000)x 100 ml sample -water reference standard ml ml ml -0251E -Nx 500

4.5 S.U.

number number (E) (acceptable range - 0.013 -0.022) 5b3-Itojll 1 IMIC ° 5 I.1 IZ Ue % SaZz -V o ~ ~ 0.1 a 4 5 Lzborato control standard: Referene True value Sample AIkalinfly(MV) %RS-MVITVx 10 stard number (TV) volume Begin End Total Multiplier (mg CCO3,/L) (aceptabl ringe (mg CaCOVL) (ml) ml ml ml -S0to 11%) W_ 615 s 6-5 100 100 u o _L c.o too toy Duplicate recision: Sample Alkalinity %RPD Sample Sample ED volume Begin End Total Multiplier (mg CnCOjL) H(S -D) I(S+DY21) x 100 number (ml) ml ml mil (acceptable range - k 1 %Y.) Duplicate 4 _________oz__________l-Matrix spke recov e., Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOCWL) (mg CsCO)JL) (nl) ml lml ml l_l_I U S 6 5 1 1__ .0 MM_ 1ZI - l Sanple alkalinity (B) lMeasured spike value (MV) % R MV I SV x 100 (mg CeCO,/L) MV-A-B (aceptble rnge I (mg CsCOj/L) 75 to 125%) Z50 Sample measurements:- Sample volume Begin End Total Alkalinity Sample number Sample ID (Ull) ml ml ml Multiplier a(mg CCO0IL) &-2A-0L 12ALA i T o to (4.1 AO.o T ____ to I LL O te0 i 55 MRS 16zaA _1_ I?.1 Ja-1 1To (I VID-4i)D>lfi 3 lll 'l1 -7l1'tl 1 l l I14 1__1I M £~~~ 2at reviewed:- Reviewed by: Il 1z Dat e d Date reviewed: ro-l-e-04 71.

I I - I, iI' Page 4z PaVP 7 of 2-Environmental Testing Solutions. Inc. Alkalinity (EPA Method 310.1). Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst CP Date analyzed [^ 1 ji,64 Titrate samples to pH = 4.50 S.U. ZXfrant ormsalityand uldtplier deferatination: pH Nz,- ormallty -Normality (A) of l2SO41 pH Factor or Multiplier Deionized Tltrnat cla,-en End Total (5 ml Na2COj x 0.05)yE (I [H S0000)/ 100 ml sample water reference standard ml -- Mnl 0.2S/E - Nx S00 4.5 SMU number number 0t i~ .e018 -0.022) Laboratory control standard: Reference True value Sample Alkainity(MV) %RS-MYi1Vx 10 ctandard number t) volume Begin End Total Multiplier (mg CaCOVtL) (acceptable range (mg CSCO,/L) mX1) nl ml ml 090 to 10%) X35S IK 100 100 10.2 Zo i .o, DuPlicate sam Il precision: Sample Alkalinity %RPD = Sample Sample WD volume Begin End Total Multiplier (m&g CCO,/L) t(S -D) Ii(S+D)12) 1x100 number (Ml) ml ml ml (acceptable range IO 10%) Li bO I f too zi [r n q 0Om s Zq c Duplicatc D Reference Spike value Sample l l t (A) standard numberl (SV) l volume lBein End Total Multpljer (mgCRCWL) (mg CaCOAL)..(ml) m l a niml Sample kalinity (B) Measured spike value (MV) lye R - MV/ SV s 100 l I CaCOWL) I MV m A

B (acceptable range I

(mg CaCOzIL) i75 to 125%) Sample measurements: l Sample volume Begin End Total Alkalinity Simple number Sample ID (ml) ml ml ml Multiplier (mg CaCO>tL) IOO0OD+1 SD5 5t 1~ -3 I C L1131t 1D 1 t I001W.oI01 LA

Lft, l

13 l 141 l5A l9 - l SB D o 'ti.D 1 C I I.Q l154to 10.5 1_10___ q-io&.o soJ-0 ~A2.o ________ID IonooW.CNQ £ I ISB 3061 q 1 1 1 rz, I Reviewed by: 1 Dae r Date reviewed: I 01-1-C-OHI

Page 35k Page I of 2. Environmental Testinl Solutions. Inc. I I I I I I I I A C.. I. '-S.. I Total Hardness (EPA Method 130.2) Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst [ A*AAl Date analyzed [ i1 l2 II rtrant nor alit and intl dier determination: Titrant Normality check Begin End Totl Normality (M) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.21E - (N SOO0)1 S0 ml sample number number _(E) (acceptable rnage -O Olt -0.022) -Nx 1000 Itab I 15$ IS 41 Z .O 0l 0.0 Laboratory control standard:_ Reference True value Sample Hardness (MV) % RS - MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (Mg CaCOIL) (acceptable range (mg CaCOIL) () ml ml ml

90 to 110%)

M5-llot40 so C>- ^ sw 112i' Duplicate son Pk precision: Sample Hardness %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) (S - D) I[(S+DY2JI 1 100 number (mI) ml nl ml (acceptable range 1* 0%)

Co4, A

11,It. tSAq 4,1 7-M s

t-£,.,

Duplicaw 6 A L. D 84 Matrix spike recovery: Reference I Spike value Sample Spike hardness (A) standard number (SV) volume Begin l End l Total Multiplier (mg CaCO31L) (mg CaCO,L) ml) ml ml ml 1"SS 000 40 to', l54 2zo l t0. Z04 % 24 l Sample hardness (B) Measured spike value (MV) % R - MV ISV z 100 (mg CaCO31L) l MV - A -B (acceptable range l (mg CaCO3/L) l -75 to 12S%) 1 _34 _ I+ 1:021, Sampl measurements: Sample volume Begin End Total Hardness Sample number Sample ID l (l) ml ml ml Multiplier (mg CaCOAL) I Blank 5s NO (should be - 0 mg CaCO)l On lO b ll hD W A -L (01AS th- _A__ -ZQ ( &.1 AL 19* lOts 12.0 -2 _ 1 1 L Im o A -?. I is 4t I 84 S56 6 ?. - 1 C 4 I 1 84 1: I b L.. S Q T_ T- -1 tD-1e Io 1 1.5 I-14h t Ptt i b 6 .0 23 a t A h >l ~ .a I l 'I Ct. 1e414 l Note: If >5ml ortitrant is used sample must be diluted. by: Reviewed I E3,_ Z Datcreviewedj

_ I_

Page 37 Environmental Testing Solutions, Inc. Page Z. of & Total Hardness (EPA Method 130.2) Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst [I amZ-- Date analyzed I on 1 ii 4 Titrantnormalfi ann i ermfnadon: Tltrant Normality check Begin En Normality (N of EDTA pH Factor or Multil reference standard ml ml l - (Kr 50000)SO0 ml s

number (E)

I cephtable rngee i -Ni 1000 Laboratory control standard:. Reference True value Sample Hardness (MV) % RS - MV/ 1 shndard number cm volume Begin End Total Multiplier (mg CaC0u/L) (acceptable m (mg CAC03/L (ml) mnl ml ml-S0t X5 ______________________40 50 Duplicate san PIc precisbon: Sample J Hardness %RPD Sample Sample D) volume Begin End Total Multiplier (mg CcC0i/L) t(S-*D) /l(S+Dy/2jj number (ml) ml ml ml (acceptable range i iA4oS L^ W Q 0 n4 ej 2 4 0;-! -. CIO Duplicate 4 D264 [46 1 D 0 arix secove: Reference Spike value Sample 1 Spike hardness (A) standard number (SV) volume BegIn End Total Multiplier (ji CSCo 3IL) (mg CaCC,/L) (ml)l m! ml ImlII Sample hardness (B) Measured spike value (MV) %R - MV ISV x 100 (mg CsC0/LI l MV-A - B (acceptable range (mrg CaCOL) 75 to 125%) Sapk Snasmprelents l! Sample volume Begin End Total Hardness S le number Sample ID (ml) ml ml ml Multiplier (me CaCOIl Blarnk l (should be 0mg CaCO3/L) z NO lI0fbD l zQ, I 3 5I 5 MIJzIII Date°[ be oe~tb Tm Cd CU l 3.l7oli l .-.Note-It >15moftitrant is used, sample must be diluted. Reviewed l xl Datereviewed by: 0 1100 C 10%) .Ii

Environmental Testing Solutions. Inc.

1.

Page J :. Page I of Z Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL - 0.10 mg/L Meter: Accumet Model AR2S pH/Ion Meter Analyst LPA Date analyzed [.I I1L] lodidereagent: !11l Acidragent: IIN 1u] Coibradon: I Reference-itandard number l- -A KAle: For samples with a residual chlorine of= 1.0 mga, die calibration range must be austed t bracket the chlorine levels of the samples. Laborarog control standard. Referencerstandard True valeTV l Measured value (MV) %RS-MVITVzl00 number (mg/L) (mg/L) (acceptable rne-90 to 110Ye I "Ss 1q7 0.5 05.' Dupicate sampieprecislon: Sampk Sample ID Sample characteristics Residual chlorine YRPD - ItS - D) [(S+D)1211 z 100 number I (acceptable range - A 10%) {YD401 .04 Asku...... ls YtGe cv, Oaz 4;Jor pS k.a......... 3t I Duplicatek D.0 15_ Sampe Measuurements: Sample Sample ID Sample characteristics Residual chlorine nbmber__ 1 Blank (should be" - 0.10 mg/L) zO Io bm vQA M o 44 I 6.s 0 O4>1tiaO I 0 k __!__iCo CA 2+ 1. Q oi' <-e (-__R <_._0_ St_ Now All saples wi alyzed in excess ofEPA tecommnd lolding tme (t5 nminutes) unless atherwise noted. 0 aep?- JOS~ I-of Reviewed by Date reviewed

Page 1_ _ Page Z of Z. Analyst W analyzed 0-1-6-7-0E Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL -010 Img/L Meter: Accumet Model AR25 pH/Ion Meter Iodide reagent [ 14 e t 77 1 Acid reagent [ I, I .the 1.0g mgeL - - I 3'L, the calibaion inge must be adjusted Z bracket die chlorine levels ofthe umles. samlyle Dlem~uremen&s: Sample Sample ID Sample characteristics Residual chlorine number (mdL) stt,^,gt Blank (should be < 0.10mglL) m j o h.t~v tc5 oo cdte n AeV cLs co-oozP'9 Dolmbz 5bs lo t ~

~

&-s .,,PJ I46fb> {)0 oltib D~oirIWA& pW.b& 4z l <O.O0tVL4. Note: AlU saleswcre alyzed In excessofEPA recommendedholding tme(15 minutes)unless othevwisw noted. Laboratory control standard: Refereuce standard True value (TV) Measured value NV) % RS - MV/ TV x 100 number (mgL) I (mgL) (acceptable range - 90ob 1101%) loss I 0.50 0.4b q7. / Reviewed by I. ZZ] Date teviewed I 7D 1

Environmental Testing Solutions, Inc. Page I16 Page I of Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL - 0.10 mglL Meter. Accumet Model AR2S pH/Ion Meter Iodide reagent: [jjiqI. ] Acidreagent: l 1FjV17 1 Analyst E fto Date analyzed la. o9. 0i Calibration: I r 1 i0.10 I 1.00 m I I Reference standard number 11j5S ;q7 l I j5s III I NAote: Forsamples with a residual chlorine of > 1.0 gL, de calibrtion tange ust be adjusted to backet the chlorine lvels of the samplc Laboraory control standard: Reference standard True value (TC) Measured value (MV) %RS - MV ITV z 100 number (OgWL) (mg L) l (acceptable range - 90 to 110%) It 0.50

0. 51 i 103. o%

Drlicate sample prcislon: Sample Sample ID Sample characteristics Residual chlorine %RPD - ((S - D) /[(S+D)j211 x 100 number 4 L) (acceptable range - a 10%) 2i N 0 1Asy4. 1J otol e( Ci S _ I Duplicate D NO_ Sample measurements: Sample Sample 11 Sample characteristics Residual chlorine number (In__L) - Blank (shouldbe-cQ10m0 L) < 0o.oo9l 401OG. Oz 50Q? 10 do Col06r, ia

.Do 7

oC5 g.o 6

Note.: AU! samples w.r analyzed in excess of EPA itcommended holding tisne (U5 ainates) unless ofthwise noted. Laboratory control standard:- I Reference standard True value CMV Measured value Om) %RS-MV/TVx1OO number I(U19L) (MgQL (acceptable range - 90 to 110%) I .o 0.50 qq 5 Reviewed by I Kw Date reviewed rfl3njJ

I f Environmental Testing Solutions, Inc. 583 Page I1 Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL - 0.10 mg/L Meter Accumet Model AR2S pH/Ion Meter Iodide reagent: [l) JRlIS Acid reagent: l 1 _lO I Analyst Date analyzed (-O. jjl C I, I '4. 1j. I I

?-I

I - I **.; 'I I 4*'t' 4'-. ; Calibration: I -': :-' ' !-; *. 1 ; ss'- "~wgL t l~g Reference standard number I 0.1sslq0 + I I.0 bGAI I Note: For sunples with a residual chlorine of > 1.0 mg/L the calibration nmpge must be rusted to bracket the chlorine kvels of the swmples. Laboratory controlstandard: Reference standard True value (TV) Measured value (M) % RS-MV/TIV x100 number (mgwL) (uL) (acceptable range - 90 to 110%) 0.50 n s

1. 11 Duii cte sample recilon:

Sample Sample ID Sample eharacteristlcs Residual chlorine %RPD - ((S -D) II(S+D)12l) x 100 number (mL) (acceptable range -J 10%) ~ L Q I. ~ Z ~ b A ~ r ~ ~ ~J a. I S £ 0. C ~ 1 O Duplicate ` _D 4____ j Sampl mJeasurements: Sample Sample ID Sample characteristics Residual chlorine number (mgVL) 0 Blank (should be - < 0.10 mglL) 02 Omi I: o % -io.o ~ a-~~r. 0.0 0 524 O ~t4 0t r f b M ' a

LO A

i c. £ O. 0 3 bLIO-io. 03 rrC6V Fx-n L) W,>*zu pjsx 1i)40 o C IV~~ '?NeA SS f loC. 010 Cuwa i0,005V1 o'Jo 0'7.OZ-P oli o no c.14&v. CUOLO 0 53 DqO?10 p 1 IV A r r ocHwy-0qon ac.o r j j j q j jL 0.Oc qc9 Note: AUl samples were analyzed in ecess ofEPA recomended hg( m s Laboratory control standard:. Reference standard True value (TV) Measured value (MV) %RSMVITVZ 100 number (mgtL) (mgL) (acceptable range - 90 to 110%) _ _ s JS5 0.50 O. 9O I 7. Reviewed by I ff

1 Date reviewed I CV4Q-11

.)

Sequoyah Nuclear Plant Biomonitoring July 7 - 14, 2004 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Piinephales promelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 I I I I I I I I I I I I I I I I I TI I State and USEPA Mandated Control Limits Limits Set According to +/- 2 Standard Deviations 0.7 0.6 9 iq 14, 19I 0I.ti.Po I I I I I I I I I I I I I I I I I I I I 0.5 0.4 F,t .1 1.0 0.9 0.8 0.7 0.6 0.5 0.4 USEPA Warning and Control Limits Limits Set According to I e and 2? Percentile CVs ..................I..................................................................................................................... 11, %.,- ;NO 001 01 Test date 0 7-day IC7,s = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephates growth for the test population. Central Tendency (mean IC.) Warn1ngLWts(meanIC25-+SA.j0) .......... Control Units (mean IC2, +/- SAM or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephelexpremde adig Moderately Hard Synthetic Water Sht41JdUSA 1sb..'t" l 1bfwtM WIJ A UlBPA Tut _"dMI Twt ask 7I-d"C, CT S CaUrd Lbt 9a WIu limb SAN Cst l Unit SA Won" lmts 8 3 A1 C I41bnts CV (fL KCO (IL :CO CT-20 S CT+2-CT-O CT Cr+SA CTS-AS CTr+JiA,, CT-SAM Cr+B, CT-8As CrT+ SAM 1 11-11403 0.53 2 124903 0.51 035 0.03 0.49 0.62 0.07 0.48 0.62 0.12 044 0.67 0.21 0.34 0.76 25 030 0.Q0 0.06 3 01-0604 0.66 0.9 0.07 0.45 072 0.07 0.2 0.66 0.2 0.6 .71 0.22 036 0.81 026 0.32 0.85 0.11 4 01-304 0.64 0.60 0O. .48 Q72 0.07 03 0.67 0.13 .47 073 023 0.37 0.33 0.27 0.33 S.7 0.10 S 01-20404 0.3 Q62 0.06 0.49 0.74 007 O.S4 0.69 .13 O9 075 0.23 0.33 03S 2t 0.34 .39 .10 6 02Q0304 0.66 0.62 0.06 051 0.74 0.07 035 0.70 0.13 049 0.75 0.24 039 0.36 0.2 0.34 0.90 0.09 7 03-0204 066 0.63 006 052 0.74 0.O 0.5 0.70 .13 0.0 Q76 0.24 0.39 0.7 0.28 m35 Qn9 0.09 8 03-1644 0.64 0.63 0.05 0.53 0.3 0.03 05 0.70 0.13 0.5 0.76 0.24 0.39 0.37 023 0.3 0.91 0.03 9 04K-064 0.60 .63 0.05 O 0.72 0.08 0.5 0.70 0.13 0.49 0.76 0.24 0.39 0.36 .2 0.34 0.91 0.08 10 04-13-0 0.64 063 0.5 O 0.72 0.0 0.55 Q0 0.13 0.50 0.76 0.24 0.39 0.37 0.23 0.3S 0.91 0.07 11 04-20.04 Q63 0.63 0.04 0.54 0.72 0.0Q 0.55 0.70 013 0.50 0.76 0.24 0.39 0.31 0.28 03S 0.91 0.07 12 05-06-04 0.63 0.63 0.04 0S4 071 0.0" 0.5

0.

0.13 0.50 0.76 .24 039 0.87 23 0.35 0.91 0.07 13 05-114 0."64 0.63 004 0.55 J0.1 O0. 0.55 0.70 0.13 0.50 0.76 0.24 039 0.37 0m 0.35 0.91 06 14 05-13.04 0.56 0.62 0.04 0.54 0.Q1 0.07 0.QS Q0 0.13 049 0.76 .24 0.39 036 0.23 034 0.91 0.07 15 05-1344 0.58 0.62 0.04 0.54 0.t1 0.07 0.S5 0.70 0.13 0.49 0.75 0.24 0.39 6 0.23 0.34 0.90 0.07 16 06044 0.61 0.62 0.04 04 0.70 0.07 0.5S 0.70 0.13 0.49 075 0.24 0.39 0.86 0.28 0.34 0.90 0.07 17 06-2244 0.57 0.62 0.04 0.3 070 0.07 0.54 69 0.13 0.49 075 0.23 0.38 0.5 .21 034 .90 .07 13 07-07-04 .54 0.61 0.04 0.52 0.W 0.07 OS4 0.Q6 0.13 043 0.74 0m 0.38 0.35 023 0Q34 .39 0.07 19 07-144 0.55 0.6 0.05 0.52 0.70 0.07 0.54 68 .13 0.43 0.74 0.23 03t QU4 027 0.34 0.33 008 20 07-2014 052 0.61 0.S 0.51 Q70 0.07 .53 0.3 0.13 0.43 0.73 0.23 0.33 0.54 0.27 0.33 03 0.03 NOW 7-4 IC,.- 7d 251 4-a emoh Autsthm.t tat 25% m l hPie_ g fitbe ttp CT-1kmwdnlba7J:..O. 8 - 81nrdd doibt fffie,. vnhe. 1.tcy wu ng wbW~fft04dUhIdwitdw diitn d w tefthe uleqa4tOdi 10.mXd2Muein C..Th.. pa-sihatfi.. iemmr mdadhgus ueinedeU S !PAf@edi t..tineo l d mde4. SA8d-3ffaVGbft9 srpbg 0Ipat&CV. (01-an) pM 1t~dwdd~pbn O"pOMigfo fie a 2 CV. (8M-0.21) UPAC.tudWa Ina ft SA ,,- t.dwdn aetI ew-potglew 7pA CV.(8A -033) SM !tininfld QW"en PMudag9b 0epennlkCV'. (NA.O-OAS) CY - roem utrM. at fwc, Vakw. tfA. 0 tlMdfmdAiw.Ekk tletlW ndf gbd h t l3tpbxbPA4033&-MM UN fIti WoaA ~ ahI OI hindrk dja 07-2

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromdlas using Moderately Hard Synthetic Water Teat aumr Test date Control Control Mean Surviv GoWth Cr CV cr IMS PMSD CT (X) (MZ~fM) for Contrul Ckow&h (invurve) M%) Orow&hCV (%) (%6) for PMSD (%) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 11-11-03 12-09-03 01-06-04 01-13-04 01-20-04 02-03-04 03-02-04 03-16-04 04-06-04 04-13-04 04-20-04 05-06-04 05-11-04 0548-04 05-18-04 06-08-04 06-22-04 07-07-04 07-13-04 07-20-04 100 100 97.5 100 100 100 97.5 100 100 100 100 100 97.5 100 100 100 100 100 100 100 0.9S3 0.744 0.816 0.807 0.770 0.847 O.S66 0.686 0.708 0.780 0.784 0.832 0.6SS 0.718 0.681 O.8S9 0.700 0.610 0.805 0.727 0.848 0.838 0.830 0.818 0.823 0.786 0.774 0.766 0.768 0.769 0.774 0.76S 0.762 0.756 0.763 0.759 0.751 0.754 0.752 11.6 2.2 4.6 3.3 16.5 5.2 4.8 7.3 12.0 9.8 5.5 5.5 13.1 4.4 9.3 1.3 15.1 11.1 10.2 15.6 6.9 6.1 5.4 7.6 7.2 6.9 6.9 7.5 7.7 7.5. 7.3 7.8 7.5 7.7 7.3 7.7 7.9 8.0 8A 0.20 0.18 0.13 0.10 0.13 0.11 0.08 0.08 0.14 0.11 0.08 0.10 0.11 0.08 0.12 0.08 0.12 0.08 0.12 0.17 21.3 24.2 15.5 12.5 17.0 13.0 14.8 122 19.3 13.9 10.2 122 17.1 10.5 17.8 9.6 16.8 13.6 15.4 23.4 22.7 20.3 18.4 18.1 17.2 16.9 16.3 16.6 16.4 15.8 15.5 15.6 15.3 1S.4 15.1 1S.2 15.1 15.1 15.5 Note: CV coefficeat of "nration for control grwfdL. On rage, Me CV for control growlh i 8IA% in Environmental Te ing Solbtions, Inc. PFm4hilks chronic toxicity test. Lower cV bound determined by USEPA (l0e percenile) - 3.5%A Uppa CV bound determined by USEPA (90' peeanile) - 20% MSD - Minimun Sigafficant Differce PFMSD Percent Minimum Signiant Difference PMSD is a measue of test pciso The PMSD Is the ninim percent difference betwen the control and treatment dat can be declared statstically significant *na whole effluent xicw test On avrage, a signifcant differcnce occun for En-miental Testing Solutions, Inc. chronic toxicity tess when a toxicant reduces PFwhalar growthby 1S.5% firom fg controL / Lower PMSD bound datennined by USEPA (10t percenfie) - 9.4%. Upper PMSD bound detemined by USEPA (90' p benle) - 35% CT Central Tendancy (me Control Growth, CV, or PMSD) The lower and upper bounds weiu calculated by dle USEPA wing 205 eIc conducted fro 19 laboetrics for Phnephals growfliin chronic reference txicant tests. USEPA. 2000. Understandin andAcunitg for Meod Vably inWhol Effu=tToxicityAppcat s Undr Ie Naonal PollutatDisage Blitinn Progam EPA-433-R4O003. US E hvimnnentl Protection Agency, Cincmiat, OR Orgaiztrm otb~zhedfrom Aqua&c BioSyst=Am Inc.0-00 07-20-04

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 ,ON ... 4 U X 9 in C4 U PC ,it 0.7 0.6 0.5 0.4 .ow a I 0.3 0.2 Test date 7-day IC25= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC2.) Laboratory Warning Limits (mean IC25 i S.,I0, S, = 0.12) Laboratory Control Limits (mean IC5 SAM, SAM= 0.21) _ _ USEPA Warning Limits (mean IC2. + S, 7 5, SA.7S = 0.38) USEPA Control Limits (mean IC2. S%, S% = 0.45)

Environmental Testing Solutions, Inc. Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 1.25 II III III I I I I I$ I I I I I IT I. I T F 1-1 1.00 0.75 0.50 0.25 _.._i._.m A_.._t c Criteri (0_. 2 per .. _..ng Minimum Acceptance Criteria (0.25 mg per sunviving larvae) I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I m0. ar 3 O 9 g 40 _.................._._._._._._._._._._._._._._._._._. North Carolina Acceptance Limit (< 40.0%) Kentucky Acceptance Limit (< 30.0%) 20 e0 Test date a Control Growfth or Coefficient of Variation (CV) Central Tendency (mean Control Growth or CV) .. Control Limits (mean Control Growth or CV M 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 40 30 1 00I a 1 1 1 I I I I I I I I I I I I I I_ I 20 10 0 &-L -r Test date -e-PMSD - percent minimum significant difference. PMSD is the minimum significant difference between the oontrol and treatment that can be declared statistically significant Central Tendency (mean PMSD) .Lower and Upper PM SD Bounds Lower PMSD Bound (1 0^ percentile) - 9A'4, Upper PMSD Bound (90e percentile) 35% (LAwer and upper PMSD bounds were determined by USEPA for the method and endpoint)

Environmental Testine Solutlons, Inc. Page I of 5 Environmental Testln2 Solutions. Inc. Page lof 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Piinephaekspromelas PpKCICR Test Number: AS Diludon preparation Informaon: Comments: KCICHMnumber: CIAM l'42 Stock preparaion 50 g KC/L Dissolve 50 KCIa in l-L Deionized water Dihtion prep (mt/L) 300 450 600 750 900 Stockvolume (mL) 6 9 12 15 is Diluentvolu wniL) 994 991 983 985 982 Total volu=e(ML) 1000 1000 1000 1000 1000 Test organism Infornaton: Test Information: Organism ge: 2L.Sm 2a t 0 : Randomizing tplate: 6Zj Dateandtimesorganisns 01 ot-Atq -MW WD I i Incubatornumberand were born between: Mao mIO MOe r shelf location: Organism source: ASS B 0-c -ol4 Anemia lot number: A I OSS Transfer bowl informationm pH - SU Temperature - C Total drying time: s _.__q 24.5 Date/ TIme in: 01rL-V 14N5 Average transfer volume: Date / Time out el=& Oven perature: Dailyfeeding and renewal Information: Day Date Morning Afternoon Test InItIatIon, Control water Analyst feeding feeding time renewal, or batch used time termination time H4lZ o 01c204

M-q Ioc 1-6 l

CILI 1 l -Zl-mQ0os ISt; 19. 01nX~ - 6 2 u2ivoq t>1S I'S 0 t1tsb= 0o1-Iq -t1 3 23 O&S iSil o V4 0-cS .4 01-2 o&l t0516 VS2.2 1t6-1 01-11-C!tA 01-z$ o t'Sco J0L Z 15C, 6 al21-V S% r s Control Information: Acceptan e Summcry of test endpoints: % Mortalitpr. OI) s 20% 7-day LCsq 331f.l Average weight per initial larvae: 1 e.12b NOEC -q50 Average weight per surviving larve: 0.1 2 0.25 .me LOEC 6 00 ClV IC2s I 5z.

I Environmental Testing Solutions, Inc. Species: Pimephalespromelas I:. Page 2 of S PpKCICR Test Number. q Survlrd and Growth Data I 111' I .1 I I i I I I I Day CONTROL 300 w KCUL 450 m KCUL A B C D E F I G H I J K L 0 to JO 1_ t0 1o 10 10 10 10 1O 10 to I 10 to IQ to /0 (0 Io /0 /6 /0 /6 /0 /2 /Q jo Io Io lo 5o S1o lo /0 D A 3 lo / Ito 1 _ I 1 to to l / 4 JO io/ 10 10 do 1O6 It J O S 1D / a jo I 10 1 1t/0 1o 6 J /0 /0 10 10 lo 10 lo /0 /0 JO /0 /0 /0 10 10 / 16 0 o to0 t /0 /60. 7 ,0 8t 0 _6 to -( 1_ It o o t 1 o' A - Pan weight (_g) TAAY el isx 6*. r oo s %6AX, 401% "Jq 14 16.0f w*w 6% B-Pan + Lame weght (MO ZZIS4 21.5 .2Z 2t.65 11.61 tZ.80 Ul.o 2s-I 11.15 ZZ.11 Zk28 ZoK C-Larvaeweigbt(mg) -A-B 'lb Wedght per Intal number of A _e l larvaefmg) ~~V ~ ~ .~ - Cl InIialm umber eflarvae Ir t O f' O. Ot Average weight per initalt sumberof larvae (mg) .17 VI o.516 a. Calcutations and Atar rvlewed. 6\\ Comments:

I I.. i I I I I I I 1. I I I I I I I l

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Page 3 of 5 Species: Pimephalespromdas PpKCICR Test Number: Ao Comments: I. I -

Environmental Testing Solutions, Inc. Chroni Whle Adft To*It Tet (EPA4214t041X Metod 10d Spey:rn Pbepwespvfmdw Q112ifty COtrlM Vel'fICatleR Of Dta EnRy, Calculationst, and StaIdtial Anal"e TatmOu_ DpKofseetwnftwEL Tat ak = Ay l-27, 1 Jtfwlw#dbp= %-.01 I 0 z ~" bw l#-i_ ^. S-a to 0 1489 22Ss 7.96 .794 0.7_6

0.

80 10 14.93 21.38 63 0.63 0726 15.6 0.638 IOU s7i SAS NM *"snbat C 8 t IS."0 2.2 62 0.622 0.422 -D 10 10 1i.s6 2363 80.49 o _0.549 1 80 10 15.13 21.87 6.74 0.674 0674 to i s3.21 22.80 759 0.7t7

00.

e759 ^ c14 21-7 634 1 0.634 d634 I n I_ 14n 2297 7." 3 0.74 079 I. J 104.73 19.95 527 0 07 4.27 4" H 80 o 4.4 227 799 0-504 0575 22_ 0.799 2AZN L to 0 84.68 It I 5.02 0 27 0.500 t3.04 21.24

63.

0.620 e 620 OV l ow 14.70 20.13 5.43 ea1 s.l 0571 1.IO 4 X t 2# o-l 14.59 22.1 76 0.69 0.49" IF t 9 14.32 _n0.442 0399 802 14.26 83.32 8.26 0.630 0.126 7I t0 4 14.76 16.11 8 13 S 1-- St 25 1 0.33 nJU g6 31 7T -S= t0 4 13.32 1735 223 0.3 o23 10 5 41A6 1624 1.39 0.460 O_.3_ U 10 o o.oo 0.03 0.000 0.000 t V L. 2 .47 I4." o1 807 0.485 0.650 20.6 0.097 iRs IIn" "A W o

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000 000 0o00 o.00 0.033 0x 1 814.62 8527 065 0.650 0.065 -SD D r111% 2ISA 9MS-Po Mepwmi6D88hsw.ss Lo198bw4uwhbyWA(00,pw_)h 9.4% (JNwSDft4S 4 0,W elile)33% Thalwumiqipaeid vwa-IduulbydeUWAtwft205 4.nemluui Wt 89 N~uI kelrra.qlid pw6,h imm.fowukwconwiL, use& 2800.Ux.044.4UA~mlMU-

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Environmental Testing Solutions, Inc. Statistical Analyses LArval Fish Growth end Survival Test-7 Da Survival Start Dat: 7/20/2004 Test D. PpKCICR SanO1lD): REF-Ref Twicont End Dat: 7=/20 Iab 1D. ETS4nvir. Testing Sol. Snple Type: DMR-Dicgo Monitorng Report Sanpe Date ProfltE CHRONE43PA-821-R-02-013 Test Speciee PP-Pinepbales prwnelas Conmynb: Caue-iL 1 2 3 4 DCol 1.0000 1.0000 1.0000, 1.0000 300 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 1.0000 0.9000 0.8000 0.9000 750 0.M00 0.4000 0.4000 0.3000 900 0.0000 0.2000 0.0000 0.1000 Transform: Aresn Square Reot Rnk i-Tailed Number Total Cone"eglIL Mean N-Mean Mean Min Max CV% N Sunm CrfitCal Resp Number D.Cono l 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4 12.00 10.00 4 40 0750 0.3250 0.3250 0.6032 0.4636 0.6847 17.472 4 10.00 10.00 27 40

900 0.0750 0.0750 0.2757 0.1588 0.4636 53.294 4

10.00 10.00 37 40 Auxiliry Tests Statistic Citical Skew Kurt Shaphro-Wift Ted idaod"s non-nonnal distnbution (p <- 0.01) 0.84628701 0.884 0.2491888 1.10337554 EBqaity of vtrin cannot be confirmed Hypotheds Test (1-tfl, 0.05) NORC L4DZC ChV TU Steels Many-One Rank Tes 600 750 670.820393 Trmtentfs vs D4C3otrol Organisms obtafnedfiom Aquaic BioSystems, Inc. 07-20-04

Environmental Testing Solutions, Inc. Statistical Analyses C"Am aq C~ promefew Vd. St 9% MWMUelt P.,.w Mu Mr" 11w 1-5.84661 1.12162 152952 2.92 -402mo8 6.32972 52.69 -2l.M"424 0 0.72310589 7.814727 087 133966 0.0630331 3 Pu blt RngL 95% FIuiwI ECOl 2.674 5".717081 U3.04463 554.15391 PaMs 3355 6216" 5.17121U 60.64266 ECtO 3.718 593.174 542.93189 627.549 BCU 3.964 614.6633 568.M336 648 2 PCM 4.158 632287 50.0 66860 PCMS 4326 647.8582 601589006 67292264 PM4 4.747 68647563 65.87 6 717.21549 DM0 5.000 714.440M66 63061874 744.57094 BC60 5.253 74L200262 71.532092 77494 BC75 5.674 787.91892 755.3615 833.036 LCMO 5.842 807.265421 772355443 85.644309 BCJI 6.036 830A6525 10082 301007 BC90 6.282 860491423 816.737752 932130I PC95 6.645 907.0173 8579508 99933615 ECM9 7.326 101.39037 92."6377 114L1109 1.0 0.9' 0.8 GL7* aQ4 03 0.2 Q.1 U.U.. low 1loo0 1 10 100 Do. nVL D Fit Org-anf obtaftedfi Aquak BoSt Inc.70 07-20fi04

Environmental Testing Solutions, Inc. Statistical Analyses Larval Fish Growth and Survival Tet-7 May CGowth Statt Dte: 7/20/204 Test H. PpKCICR Sanmple D: PIF-Ref Twdoant End Date: 7Z7/204 lab Mt ETS-Env*r. Testing SoL Sbnple Type: DMR-Dchasnrge Mowoitng Reprt Sample Date: ProtoaL CHRONCY-EPA-821-R.02-013 Tes Species PPPimephales premela COM4C/L 1 2 3 4 D.Control 0.7960 0.6390 0.6220 0.8490 300 0.6740 0.7590 0.6340 0.7990 450 0.5270 0.5040 0.7690. 0.5000 600 0.6200 0.5430 0.4950 0.3980 750 0.1260 0.1350 02230 0.1380 900 0.0000 0.0970 0.0000 0.0650 TransforNm Untransformed 1-Tafled Isoto"nC Coue-yL Mean N-Mean Mean Mh Max CVe N t-Stat Critel MSD Mean N-Mean D-Control 0.7265 1.0000 0.7265 0.6220 0.8490 15.576 4 0.7265 1.0000 300 0.7165 0.9862 0.7165 0.6340 0.7990 10.576 4 0.135 2.290 0.1699 0.7165 0.9862 450 0.5750 0.7915 0.5750 0.5000 0.7690 22.588 4 2.042 2.290 0.1699 0.5750 0.7915

600 0.5140 0.7075 0.5140 0.3980 0.6200 18.075 4

2.864 2.290 0.1699 0.5140 0.7075 750 0.1555 0.2140 0.1555 0.1260 0.2230 29.124 4 0.1555 0.2140 900 0.0405 0.0557 0.0405 0.0000 0.0970 119.891 4 0.0405 0.0557 AurIary Tests Statiste Crtlical Skew Kurt Shapifo-ilk-s Test indicates nonal dibntrbution (p > 0.01) 0.9246071 0.844 0.59174542 -0.7397034 Bartets Tes indicates equa variance.s p - 0.84) 0.83600086 11.3448668 Hypothesh Test (1-hal, 0.05) NORC LORC ChV TU MSfu MSDp MSB MSm F-Prob df Drknet's Test 450 600 519.615242 0.16991969 0.23388809 0.04431933 0.0110115 0.03399318 3,12 Treatments va DCOntrol Organ0sms obtafnedfrom Aquatic BloSystems, Inc 07-20-04

Environmental Testing Solutions, Inc. Statistical Analyses 1ww MOL SD 93% Ca ) Sbw 1M 327.91 074 0.00 495.27 -07679 1010 346641 67.17 67.59 M5.19 43917 1015 40.2 59.8 1.20 "Ca67 o.O t.0 04433 6664 2%063 695.99 0.3415 1X:2 524M0N MU34 "5 .42 .0.1493 09 040 6316

23.

516.02 66&01 -17043 0.K lC50 663.01 1332 613.12 692.39 .70 0 0.6 0:0.4, 0.3 .2: 01./ 0.0 0 200 400 600 800 1000 Do. neL 0.8 0.7 I-MR, l M .............I of 1k ~ ec Orgnm- -bt Trwdfi-Aq-d-*Btogyan Ilna 07-20-04

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Page 4 of S --1. >: Species: Pimephakspromdas PpKCICR Test Number: O Daily Chemistry: _1 I Day I I 0 I 1 I 2 I Concentratlo Parameter _~. CONTROL on (S.U.) z DO (mztL) s Conductivity (umhos/cm) .5 AlkalinIty (mg CaCO3/L) -7. l 1 7-5 175 7.7Y 0-1lfh 0-14 R1 zsq Z;14 SA9 S 6el -4; 25.\\ 4^ a S. zi. ZS.0 ?A.( ftardness (m_ CCOAU 4-Temperature (-0 pH (S.U.) § w st., DO (mglL) 865 7.0 l 7TV 300 mg KCIIL Conductivity (Thos/cm) 7 3 l Temperature 24.s -0 21 nCH (S.U.) A.o I qz I fa.Io v I 4.7 I i.oq I V.B3 I DO (mg/L) 450 mg KCIIL Conductivity (mhoscm) JI 6L4 j pk .o I ' 6.q1 7-It I 1.3 ~1110 ?ws M.'l Zq.16 Temperature (Pa pH (S.U.) 8.l

.Io 7.f o.1e DO (mgAL)

B1,7. .9 ?5 600 mg KCI/L Conductivity 3 M C(nmhos/cm) 1~O Temperature Zq .9. Z4.! PH(S.U.) .o 7.7I6 _.78. DO(mg/L) 8.4 .I6 7,q .0 7.s 750 mg KCiJL Conductivity 167O (wihos/cm) o Temperature

25.

2 q.. z.i zq.o 'aS-6 ci (S.U.) .10o I 7.Y.5 T10.W I 7145 I V-c3 I -4.IY I F 4 I? 3IIU I U DO (mg/L)

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I-. 6 Ifq 0 1 -., 1 .1 0 900 mg KCIL Conductivity (gmhocm) _ Al Temperature (0C) aqoo A1 I15 ~ tS.OW~ zq .O 2'. S *4Zg Inial it Fi00a Initial l a Initial I al IInitial la STOCK ' tConductlvlty I (Anhos/cm.)

Environmental Testins! Solutions, Inc. Page S of S E-iomna -etn S-uios Inc PaeSo Species: Pimepholesprometas PpKCICR Test Number: _ 3 4 5 6 An alyst rcS I 15 fe is lo ?C1 % Om Concentration P arameter_ CONTROL jpH (S.U.) 414t.WX°L18 ~ ~ DO (m2/L) 7 l .5 M '7. Conductivity AZe Hardness Temperature DO Ag/l) IT. 2 z'.I ZSSA 2 6 300 mg KCa/L Conductivity Temperature Z 2s. \\ _ p(S.U.) 8o-T 7.5 . 0 0 1.9 DO VAp. L. , o 1 B .O I

1.

11 450 mg KCI/L Conductivity 1 103 Temperature .z,.tit 4.2 'ZA

2.

2q 250 M5 mg KCLCnutrA 13° O O3o 13 p}I (S.U.) 6g S .? Ro ,05 I., a-s.o -1.615 600 mg KCI/L DO (mg4L7. 7 M Conductivity Temperature Z5. I Z.'z. 2Z. 2 25.0 4.j PR (S.U.) 0._ 7!7 8.01 -LB 615bs-010 78 DO (ngWL)

7. Ir

7.

.77Z 6 7 B 750 mg KCI/L Conductivity Tempenture t . 2. Z Z.O 2B 2.1 2 (M) pH (S.U.) 74.1 8.00 I 1.&h I g.oq I '7.18 1 A-in 11.89 I

6-a
I*.---=---1I-DO (mail) 7A 1 *1.0 - I 7.?L LI -

7 1-8 I 900 mg KCU/L Conductivity (tmhos/cm). i Temperature M 203c Mt>660 q& mSt 24.G -2.Z z .z ZS. 1 Zsl-t

2. 0 Z.

7390 1S.o

900 Itlfal Final Intial Final Initial Final

-Initial I Final STOCK Conductivity _ I nffmhslcm)

Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnla dubia using Moderately Hard Synthetic Water 1.20 1.15 1.10 1.05

I I

I I I I I I I I I I I I I I I State and USEPA Mandated Control Limits .Limits Set According to 4 2 Standard D ev ai s............................... l,..... f i (UV z tU %%00 1.00 0.9S 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 w I I. I I I I I I I I I I I r Laboratory Warning and Control Limits Limits Set According to eh and 25 Percentile CVs Jt o ZS Jt" otSA 0 20 JO 0 ! .0o0T date Test date 7-day IC, = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphna reproduction for the test population. Central Tendency (mean IC2.) Warning Limits (mean IC25 k SAd1o) .Control LmItS (mean IC25 4 SAM or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toicant Control Chart for Ceriedapbrni dabia using Moderately Hard Synthetic Water Tet wme Test t 7-dy WCm CT (AMM~ btLlqo 8hh1. NMUMA B CSewtsLfni CT-28 CT+28 WaCT n8Lhit T+ M CT -SOA Cr +SAA* CT - PSA CTr+ 8AM OA" Wm 3 1.%ft CS -SIM C + SAM, 8 " C..1i b CT - 81.P cr + a8,, CV 2 2 3 4 S S 7 a 9 10 11 12 13 14 15 16 17 is 19 20 M12342 01-07403 024403 0340503 0441-03 03.0-03 0644C03 07103 085403 09610403 104703 114403 1244-03 014644 02004 03Q304 0440604 05-4-04 06-04 07414 L04 0.6 Q.9 L05 1.03 L05 L07 L03 L04 L05 1.02 L08 LOS 1.06 1.06 L07 1.07 L07 L06 L06 1.00 0.06 0.9 L2 O.OS 0.92 LO0 0.04 0.92 LOS 0.0Q 0.92 LOI 0.04 O."3 10 0.0 0.93 LO1 0.04 0.94 LO9 0.01 0.3 L02 0.04 095 L09 0.0 0.4 L03 0.04 0.95 .10 0.08 0S4 L03 0.04 096 10 0.03 0.94 L03 0.03 0.96 L09 0.08 0.95 1.03 0.03 0.97 L09 0.03 0.95 1.03 0.03 0.97 L09 0.08 0.935 L03 0.03 0.97 .10 0.0 0.935 1.04 0.03 0.97 .10 0.01 0.935 L04 0.03 0.97 1.10 0.0 0.6 L04 0.03 O.S .10 0.0Q 0.96 L04 0.03 0.9" 1.11 0.03 0. L04 0.03 0.9 1.11 0.08 0.96 L05 0.03 O.3 1.11 0.08 OK L05 0.03 039S 1.11 0.01 06 L05 0.03 039 Lll 0.08 0.96 1.03 LOS L09 10 .10 1.11 Lll 1.11 1.11 1.11 12 L12 L12 L12 1.13 13 13 13 13 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.13 0.17 0.1S 0.18 0.1I 0.18 0.13 0.18 0.13 0.1S 0.1S 0.33 0.33 0.84 0.84 0.85 0.35 0.85 0.35 0.34 0.35 0.34 0.86 0.36 0.86 0.87 0.87 0.31 0.87 0.87 117 0.45 0.55 L17 0.45 05 LIS 0.46O 0.56 B19 G.6 036 L19 0.46 0.56 1.20 0.46 0.56 L20 0.46 0.56 L20 0.46 0.57 L21 0.46 0.57 L20 0.46 0.57 1.21 0.47 0.57 L21 0.47 0.57 L22 0.41 0.57 L22 0.47 0.57 .22 0.47 0.57 L22 0.47 0.57 1.22 0.47 0.57 L22 0.4? 0.58 L22 0.4? 0.53 L45 1.45 L47 L41 1.48 1.49 L49 L49 L49 L49 L50 1.50 1.51 1.51 1.51 1.51 L52 L52 1.52 0.62 0.38 0.62 033 0.63 038 0.63 039 0.63 039 0.64 039 0.64 039 0.64 039 0.64 0.39 0.64 0.39 0.64 039 0.64 039 0.64 039 0.64 0.40 0.65 0.40 0.65 0.40 0.65 0.40 0.65 0.40 0.65 0.40 L62 0.06 L62 0.04 L64 0.04 .64 0.04 L65 0.04 L66 0.04 L66 0.03 L67 0.03 1.67 .03 1.67 0.03 L67 0.03 1.63 0.03 1.68 0.03 L69 0.03 1.69 0.03 L69 0.03 L69 0.03 169 0.03 L70 0.03 Nets. 7.4 JC,, 7.d u 2 S% h N t m m e n r t u A ae d u t ff l u c.e mtbu e e f se d l e M ~ O t ht w u ~ e 2 5 % e s u i h ( C *w o e p a f f u u c i o m, es tp o p.1 " a m Cr-CeftIl ft.t y(sM MC) 3-ftwida doqiul affluh w1tm& I~ st mi arhib d a~ 4 at b _ otllf t fl u K w l u e m w o e g 1. f 1 0 & m 4n2 5 & p w w m til. C V u. h m i s a i n e ald a e t f in e a n o d w a i u n g 1hib.a f.byU9m'bArfld t mtaOldpeit HM 1-8Sldd dVbht;" OMOT0860d9 to f IO*paueffh% CV. (SAN - 0.03) SAM I SAudard deAlatlom onmsdg to e ba 25&pmeft CV. (AS - 017) US ~lA^ IhlW uIn.tnut SA91 -ta1d l dA .ee wmvaagb toG75*p ee CV. (SA", 0-45) S,- Stadurd eeaepu di90'pe h CV. ^(SA. -0.62) CV - Coo cimit fof tl orf r lwaLues. UBPA 2000.U.dudt.d jeehg As MefATysNalPe htDrmiomrm IEA-33R40403.US hubbltPtAob Am4y.Ckdui O}L 7074,f

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubla using Moderately Hard Synthetic Water Test dab Control Control Mean Ca number T6mydae ReproducUon fDr Control Man () (offSpdngefmat) Relrdaction (O Dfepa) I CV CT MSD PMSD Cr fir Control ('6) Reroduction CV(%) (-A) forPMSDDeK) I ... 2 3 4 5 6 7 8 9 10 11 12 13 14 is 16 17 18 19 20 12-18-02 100 01.07-03 100 02-04-03 100 03-05-03 100 04-11-03 100 05-06-03 100 06-04-3 100 07-08-03 100 08-05-03 100 09-10-03 100 10-07-03 100 114-03 100 124-03 100 01-06-04 100 02-03-04 100 03-03-04 100 04-06404 100 05-04-04 100 06-08-04 100 07407:04 100 30.5 33.2 32.3 28.7 26.3 27.6 25.9 29.0 33.3 29.3 33.4 31.0 30.4 30.6 30.7 29.0 28.1 32.5 32.9 33.3 31.9 32.0 31.2 30.2 29.8 29.2 29.2 29.6 29.6 30.0 30.0 30.1 30.1 30.1 30.1 30.0 30.1 30.2 30.4 7.4 7.0 8.1 5.1 6.2 10.8 5.9 11.6 6.6 4.3 8.0 7.3 9.7 4.4 7.2 7.8 6.2 6.4 6.8 6.3 7.2 7.5 6.9 6.7 7.4 7.2 7.7 7.6 7.3 7.4 7.4 7.5 7.3 7.3 7.3 7.3 7.2 7.2 7.1 2.9 2.9 2.7 3.5 2.5 3.2 2.6 3.2 4.7 3.1 3.2 2.6 3.6 3.5 2.7 3.0 2.8 3.3 3.0 2.6 9.4 8.6 8.4 12.1 9.6 11.5 10.1 10.9 14.1 10.7 9.5 8.3 11.8 11.5 8.8 10.3 10.0 10.1 9.0 7.8 9.0 8.8 9.6 9.6 9.9 9.9 10.1 10.5 10.5 10.4 10.3 10.4 10.5 10.3 10.3 10.3 10.3 10.2 10.1 Note. CV-CoefdEniont o atdionfrisconkol prodction. On rage, twe CV for control uproduction Is 7.1% in Eviourncntal Testing Solutions, c= Cadodaphnda ronic. xidty lests. Lawr CVbound deteminedbytUEPA (10 percentile) - 89Y. Upper CV bound dtuminedby USEPA (Cepercentile) - 42% MSD - Dinnm Significant Difne PMSD - PercentM mSignificantD i=c M is azne of6:strcision. The PMSD hie n pe t diffErerce betwen e Control and utnen tdia can be declaredutaisaflsignificantina ueeffluent iodtyts Onavcge, asigificantdiferene occursfor EnLvroen Testing Solutons, In. c ionic toxidtyts swhn e olocant educes Corodaphnfa reprodactionby l0.1% flneote controL LowerPMSDbounddete inedbyUSEfPA(10 pcrcentilc) - II. per MSD bound de edbyUsePA(9'patil) - 37 CT - Central Tmndancy (Me Control Reproduction. CV, or PMSD) The lorand pperbounds wr calculated by di USEPA sng 393 tests conducted ftom33 lboaories ftrCwitodaphnia ieproductionin chronic vtcrc= twicaut tests USEPA 2000. Und ndig and AAccunig fr oMethod Vaiaity inWhole Effluent Todry ApplctionUnder& the Ntioad PofluntDiaclarge Eimiiation PropL m EPA-33-R-O4.003. US Evonm==entd Protectiona Ancy, icinnati, OHL 07-07-04

1.8 1.6. 0u) U t.. 1.4 12 1.0 0.8 Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water I I. 0.6 1 0.4 02 Test date 7-day IC2s= 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceniodaphnia reproduction for the test population. Central Tendency (mean IC2.) Laboratory Warning Limits (mean IC2.

S. 10,I SALIO= 0.08)

.Laboratory Control Limits (mean IC2, " SAM I Sj, - 0.17) USEPA Warning Limits (mean IC2. 1 SA.75. SA 7 = 0.45) USEPA Control Limits (mean IC5 t SAM 9, SA.90= 0.62)

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction and Coefficient of Variation In Sodium Chloride Chronic Reference Toxicant Tests 40 35 C 4J 4 C E 'h-a .I 30 25 20 _ 15 Minimum Acceptance Criteria (15.0 offspring per surviving female) I I I I I I I I I I I I I I I I I I I 0 fC 0 o 0 -

c.

q = 40 30 20 10 North Carolina Acceptance Limit (< 40.0°%) Kentuky Acceptance Limit (C 30.0%) .................... M...................................................................................... I' I seI °slgy0~~~fi%¢3oX:~tts436 sp Test date Control Reproduction or Coefficient of Variation (CV) Central Tendency (mean Control Reproduction or CV) Control Limits (mean Control Reproduction or CV + 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodapihnia dubia using Moderately Hard Synthetic Water 50 45 40 35 0 1C,,a 30 25 20 15 10 5 0 tA.00%.0 Test date PMSD - percent minimum significant difference. PMSD is teM minimum significant difference between the control and treatment that can be declared statistically significant, Central Tendency (mean PMD) Lower and Upper PMSD Bounds Lower PMSD Bound (10' percentile) - 1I1%S, Upper PMSD Bound (90' percentile) - 37X% (Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)

1I 1. II12 I. L[tI:.: LI I Environmental Tesilng Solutions, Inc. e TPage 1 of 6 Sodium Chloride Chronic Reference Tozicant Test (EPA*821-R-02-013 Method 1002.0) Species: Cerlodaphnia dubla CdNaCLCR #: -Z'L Dilution preparation Informoton: Comments: Naa CHM Imbw. CK I tO__ Stockppamio 10 ICOgN aIL/(dissolve50gNaCIin 500S ddonlzed war) Diution m fiL) 600 g00 1000 1200 1400 Stockvolume (mL) 9 12 15 1S 21 Diluent volue(mL) 1491 1483 1415 1482 1479 Total vohme (mL IS00 1500 1 1500 500 1500 Test organism Informatlo : Tet nformation: Organism age: < 24-hours old Randomizing template: P2P Date and times organisms a-OV'-0 O C T Of-( Incubator number and were born between: elfloca[on: Z C.2 Organism source: o042A 04 A-E YCT batch: ABS 7 T Transfer bowl information: pH -120SU Temperate 'Z'C Selenastrum batch: ABS ob Daffy renewal Information: Day Date Test Initiation, Control water batch Analyst renewal, or used _termination time 0 __4 iI25~ Aic o~ 1 on.e.o'i 110 onOo.oq 2 I Io 0*o40 0 A 2 Dl.Nm*# 10,41 l -d 3 (7.10-0 103NR ai .(a 4 CnII.o4 10____ 0o4o0 o' 4 As 5 oA,8Z - +I\\Z 04t 6 bR-FIB4t los'sff Control nfzsornaton: AccW=critcd Summar of ett ndpoints: % of Male Adults: f, o s20% 7-day LC50 )'IqcO Y. Adults having 3u Broods: /0017 a: so% NOEC 100n % Mortalityr Of s 20% LOEC 1200 Mean OffspringiFemale: I33 -.3 15.0 offspring/fema1e Ch V JI .q CV: <40.0% j IC25 1035.1

I>.;: .Envirl I: I., t: I. I' onmentul Testing Solutions, Inc. Species: Ceriodaphnia dubia CONTROL Page 2 of 6 CdNaCLCR #: 2 Survival and Reproductdon Data Replicate number Day I 1 2 l 3 41 S 6 7 a 9 10 I Young produced 0C I O I Cb I ).I O I.OCl C) Adult mortality L. 'l L L_ L L7 L. E I 2 Yeung produced .0 _ 0 C 0 Adult mortality L L 1 L 3 Young produced f Q CL Adult mortality L 4 Young produced 5 3 I _'is I Adult mortality L L. t tl 1 s Youngproduced c 1 0 lZ 10 Ls *3l Adult mortality L-L 1 = l T I L 6 Young produced ( ) ll a C) 1 0 D 0 l Adult mortality l L. L L- .1 1l.l-L. 7 Youngproduced l'I 1lS I 9it 'I i 8 jj iS 1 I'T1Ii Totalyoungproduced l34 l30 31 l$ 5l. 35 i3 31 l l FInal Adult Mortality l l I t t?

i.

lJ ll X for 3^Brood s S J l e g -X l 5l r~ f Concentration: I l %Mortality: I ^ l Mean ringFemale: t3.3 600 mg NnCllL Survival and Reproduction Data l Replicate nuober Day 1 2 3 4 S[ 7 a 91 l I Young produced 0 0 l_ C Adult mortaity L tl\\ltl W -l-ltlt 2 Young produced D ) ) 0 Fl ) .. t ).13. 0 o Adultmortality U _lI L lL-e ILIL 3 j Youngproduced C) 0 C o 1 Q i V o I Aduitmortality L. L-L- l 4 lYoung produced I4 q IqISISI Adult mortality I

l.

L-L L I I s Young produced 1 .Q _~I Adult mortality L. C L C .(C C S Young produced 0 IClC) 1 C I1 Adult mortality l, L-l L l l l 7 Young produced 1 2o i-I-.L' Itr ao0 16 Is~ Totalyoungproduced Wl 3 l l4 l 33 3S 56 3lS l 5 37 l 36 Fina) Adult Mortality l L l l l I L l f CAk1 asve lConcentration.-l % Mortality-MO IMean Offspring/Female: I A3 I % Reduction fiom Control: -lt

Page 3 of 6 _MMM Species: Cerlodaphnia dubla CdNaCLCR#: Z. 800 mn NaCIIL Survival and Reproduction Data Reli-cate number Day 11 2 3 4 5 T 7 a 9 10 I Young produced 01 0L 0 C .. n 0 1 0 T Loo Adult mortality 1 2 Young produced 0 O lo t O C. ) n O I

A L

L 3 Youngproduced tic Q c rtal-4 Young produced Ln lo S Adult mortality L Z-..~.~ S Young produced I I I O1 Adult mortallty t U t U U U Ynng produced _I O 1 C)O Ad ult mortality L L-L- 1 t* 7 Yet a&produced Ig i's IS l( ZC II a I'sI ZO I 11 T6lyoung produced 35 32 .5L Q.3 0 0 .s 7-3 T1 l% FinalAdult mortalty 1 L. L L L -t: ? L. 1 lConcenratifon: a % MdaityL 0 I l Mean Offspring/Femnale: 33.6 l % Reduction from Control: -O.RV l 1000 mg NaCIlL Survival and Reproducton Data l ~Repcte number Day___ I 2 3 4 5 f 7 3 9 10 I Young produced Adult mortality L L. i U. T Z L l l 2 Young produced

0 0 T O I O I Adult mortality L

L TT L.- L I L Adultmortality ugl I 4 Young produced l ll I 3 I Adult mortality L L-Il L* Ll s Young produced l 1 l j l l 1 l 10 Ib Adult mortalty L. L-. L-L- U - s Ymung produced C 0 0 l Adult mortality L I - 7 Youngproduced 1N l-i l l 1 1 tol Total young produced VI 32c0 -2. Zi 5 1 316 Z, -3'( 3L 350 Final Adult Ifortality C t? T C ? x CAM 61e41 lConcentraion l %Mortalit 07 l Mean Offspring/Female: I.So.° l % Reduction from Control: 1.'. l

.Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubla CdNaCLCR #: Z( 1200 m2 NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 4 5 f 7 1 9 10 I Young produced l o l 0 I O 0 Adult nortaltylt 2 Young produced i.{ L..._. Adult mortiit Adult mortality

4.

j L. %.-~ 3 Young produced S0... L f 5 a.O..I.). Ž. Adult mortality L- ' C s Young produced CI. O 1 0 0 S O C) Adult mortality Il L u 4 Yeung produced l Z I 3 35 -O3 0 Adult mortality

l.

C_ L~

l.

7 Young produced to I 0 O Adult inortdlity L _ U U_ l.W U. i-Toa young produced I 1-L- 1

12.

11 4 Tebl yo ng produced iz 'z 2 O 2 Z j £' Q 13 6q @ l Final Adult Mortality L l Z 1 L-t r I Concentration: % Mortalit CyI . Mean Offspnng/Fremale: I It. 2. l % Reduction from Control: lA.A1Z I 1400 mg NaCI/L Survival and Reproduction Data -Rep cte number Day 1 2 3 4 5 6 7 a 6 10 I Young produced l l O Adultmortallty U l L U A -lt--ortality L L L 23 Youngproduced J O 1 0

1)

Q o Q Adult mortal I L i7 T L L L L L l Young produced 5 -I-Aldult mortality lL L L l U_ l L L Adult morrhlity l I Il l Concentruono U Au mMooayty: lI .Ul a l t i4I oGdc4 I Mean Offspring/Female: I 'I.'I I I % Reduction from Control: I &5.5% l

Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproductfon Totals Control DyROtC nmuber 1 2 1 3 l 4 5 1 6 7 9 10 T I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0o0 0 0 o 3 0 0 0 0 0 0 0 0 0 0 0 5 3 5 4 4 4 4 4 4 3 40 5 10 12 0 12 10 13 14 13 11 10 105 6 0 0 10 0 0 0 0 0 00 10 7 19 15 16 19 19 1 1520 19 19 17S Total 34 30 31 - 35 32 3 37 34 32 333 600 mg NaCI/L Day R~e at. amrberTO 1 2 1 3 1 4 5 1 6 75910 ao9 1 0 0 0 0 0 0 0 0 0 0 0 2_ 0 0 0

0.

0 0 0 0 0 0 0 3 O 0 O 0 0 0 0 0 I 0 0 4 4 5 4 4 4 6 5 4 5

5.

46 5 it 13 0 12 13 11 10 13 121 12 107 6 0 0 10 0 0 1 01 01 0 1 0 11 7 17 152017181820161519 175 Total 32 3 343 35 36 35 33 32 36 339 800 mg NaCIIL 3 3 4 4 a 9 l 1 0 0 0 .0 0 0 0 0 0 0 0 2 0 00 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 64 6 44 54 44 45 5 13 11 0 10 14 12 1 1 10 13 10 104 6 0 0 11 0 0 I 0 0 0 0 0 1 7 18 is 15S 20 161 19 18 18 20 17 176 TOta 35 32 3 36 34 3 34 32 37 31 336 1000 mg NaCI/L Ia Repnt Rwbe TO 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 4 3 5 4 43 5 10 10 11 10 10 13 9 12 10 10 105 6 0 0 0 0 0 0 0 0 2 7 14 15 17 15 1518 13 19 17 16 159 Total 29 30 32 29 31 36 26 34 32 30 309 1200 mg NaCI/L l SY l phl lcae unmber TOW 1>6l11 2 3 4 5 1 6 1 7 3 9 110 1 0 0 0 0 I0 0 I0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 0 2 0 3 1 0 3 3 0 0 12 5 1 0 1 0 0 3 0 0 0 0 5 6 4 4 2 5 0 0 6 0 5 0 26 7 7 6 7 1 4 10 5 4 6 37 7 5 Tota 12 12 10 12 11I 8 i 13 9 8 7 102 1400 mg NaCI/L Day RepTcTte nTmber Total 1 T2 3 4 5 6 7 1 0 0 0 0 0 .0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 j Q 0 0 0 0 0 0 0 0 4 2 0 0 2 1 1 0 0 0 2 8 5 0°0 4 1 O 0 o O O 5 6-1 51 3 4 0 0 0 1 0 0 14 7 6 0 3 4' 0 1 1 2 3 2 22 TOtl 9 5 10I 11 1 2 z 1 1 3 3 4 49

Environmental Testing Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA821-R-02-013, Method 1002.0) Species: Cerfedephnia dibla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Tat amuben CdN*C1C #53 ( A J3epot SL) Tet das ly 7-14, 2004 Reewd Co hatle Repikcate Rwbe Su. rv AvmV red-i. (HIL NS0 1 2 3 4 S 6 t (%) (effiprfugffule) m (%) "Nov) a-Com"l9 34 30 31 35 32 35 33 37 34 32 I"0 33.3 63 Not applkabk 6" 32 33 34 33 35 36 35 33 32 36 1I"

33.

4.5 -1J n 35 32 30 36 34 35 34 32 37 31 1"

33.

di -. 9 10 29 30 32 29 31 36 26 34 32 30 I" 303 9 7.2 121 12 12 10 12 1 8 13 9 a 7 10

10.

20 69.4 1400 9 5 10 II 1 2 1 3 3 4 1" 4.9 76A a3 Dpwwo M5Q " FMSDh 2-984 7J MSD-Mtmwn Siipcant Difaence PMSD-PermK Mbilmwn Slgidflamft Dfemmc PMSDhsamesoftstpreldm Thet!MSD demtumn perce difecbetwe dcntrolid betmntw tcbe dece stkacly ifiCntl a vwole effluent tacty le Onawg, a signfificat diffe occur 1fbr EvineTa Tstn Soldoeu. dwcn lmdty t tss wbtfnafodcat redduce Cwedaphnizmducdoibyl.% floatecontoL LowePMSDbo dete dbyUSPA(Iercent) -11% Upp ?MSD bvmd dmIned byUSEA (9 pecetk) - 37Y. The lower a uppboms e calcuatd bydie USEPA Wng_ 393 tts condtel 33 biwoe for C a reodctio mcinc c tee ofnt t USEUA. 200Q Uud nd A IbrMetd V-blt Wole EuetTxityAUdtreNaoml ntDdp Elbno EA334

03. US Envhro tal Prtction Arg

, Cindti. O

Environmental Testing Solutions, Inc. Statistical Analyses Ceriodephats Survivl mid Repd ifon Tt-Rrdeto Stit Dl 9 Test 1d CdNCICR S he ID: R RefTaxcvnt End D : 7/142004 Lab lD: TS-Envf. Testing Sol. SaITy e umcLbPotswi cloride San& e Date: Fktooml: CHRON7I47A-821-R.02.013 Test Spies CD-Coaa dubia Cormments: Cone-ug/L 1 2 3 4 5 6 7 8 9 10 D.Contr.O 34.000 30.000 31.000 35.000 32.000 35.000 33.000 37.000 34.000 32.000 600 32.000 33.000 34.000 33.000 35.000 36.000 35.000 33.000 32.000 36.000 800 35.000 32.000 30.000 36.000 34.000 35.000 34.000 32.000 37.000 31.000 1000 29.000 30.000 32.000 29.000 31.000 36.000 26.000 34.000 32.000 30.000 1200 12.000 12.000 10.000 12.000 11.000 8.000 13.000 9.000 8.000 7.000 1400 9.000 5.000 10.000 11.000 1.000 2.000 1.000 3.000 3.000 4.000 Trunrorn: Untrantforaed 1-Tailed leatomac Csuc"ng/L Mean N-Mean Mean Min Max CV% N t-Stat Citical MMD Meal N-Mean 13Cxntrml 33.300 1.0000 33.300 30.000 37.000 6.339 10 33.600 1.0000 600 33.900 1.0180 33.900 32.000 36.000 4.495 10 40.531 2!287 2584 33.600 1.0000 800 33.600 1.0090 33.600 30.000 37.000 6.758 10 -0.265 2.287 2.584 33.600 1.0000 1000 30.900 0.9279 30.900 26.000 36.000 9.083 10 2.124 2.287 2.584 30.900 0.9196

1200 10.200 0.3063 10.200 7.000 13.000 20.565 10 20.441 2.287 2.584 10.200 0.3036

'1400 4.900 0.1471 4.900 1.000 11.000 76.633 10 25.131 2.287 2.584 4.900 0.1458 AdiiHrM Tedst Statistic Critical Skew Vint KohnnagrrD Ted hiats nomnl diulfrttion (p > 0.01) 0.877379 1.035 0.35713203 -0.2168864 Badtlett' Tet indices eqal viances (p - 0.14) 8.24163818 15.0862722 Hypetheds Test (1-ta% 0.05) NORC LORC ChV TJ MSf MSB MSE F-Prob aft Dimnven Test 1000 1200 1095.44512 2.58407194 0.07759976 1756.42667 6.38518519 4.1E3-37 5,54 Treatments vs D.Contro 07-07.04

Environmental Testing Solutions, Inc. Statistical Analyses Ih-r ftemlad- (29 Rmie")- POW MOL SD 95% CL Skew ^ IC05 d0o IC15 ICPM I=25 1040 IC50 92444444 100637681 1022606? 108.8405 1055.07246 1103.76812 1136.23188 52.63431 21.7M3074 374811732 7.6903563 7.12311162 5.818694 819.245879 935.213816 100547731 1022.73855 104136132 109253456 1003.9133 1019.19124 103446669 10MSQO M 1065.19173 1I12399M 11453017 0.1971 -L6224 40.9067 -0.3027 Q3090 -0.2419 -0.0779 0.3

0. I 0.7 5.43527524 1125.10246 0.6-o0 s5 0.2.

0.1-0.0 .0.1 0 500 low 1500 Dos mL Dwe-1apm Met t-W% s .g a knc of sprfw o 25-la 20-a.' 12 is5. I 0 07-07-04

Envlronmental Testing Solutions, Inc. Page S of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubla Daily Ckemistrv: CdNaCLCR M Z: ow fl~ A fee&&t Il e9 io&ft. 'UI Dav I U I I o 1 I 12 1 ofCLR I 1 I 2 I ,,U Analyst I Concentration I Parameter pH (S.U.) DO (mg/L) CONTROL Conductivity (umhos/cm) -t 9 AMkaunity

(mf CaCOL)

_t o _, _ Hardness (Mw CaRCO/L) a 4' Temperature 2S I . t I W 0 II l .2.z 74.8 INm 1I pH (S..) 1.o0 IJS4 I V7s! I lT.1 I I in^I IL 1 T-7L I a I A I vJvJ ILWJA.] 600 mg NaCUL Conductivity (Rmhos/cm) 1 cZ ,I 31 24.i T.'. r1qo0 LS z-? Temperature ('C) 2S3. I I z4.6 I z4. I pH (S.U) 7 0 s .4

90 DO (mg1L) w 6.69>

t;t 1 800 mg NaCUL Conductivity (jgmhos/cm) 1 S 0 Temperature Z5 LB pHSHU.

7. /

7 7 7.55 SS t DO(mWL) t 7.6 .7 V.V 78 1000 mig NaCI/L Conductivity (wzhoslcm) Temperature 2ZS zSZ Z ,1 rc) PH(S.U.) 1_7°_ ?. T6 S. s 7.qE1. .a I DO (nmgL) i t 6 7.7 1200 mg NaCI/L Conductivim y ) 5° 2qtZ (g&mhos/cm)Vt Temperature 2I'2 Z4.q ZZ Zs.i C. _T _ I VA Z4. OH (S.U.) .4o I 4.60 1 7.ss. 1.146 1 7. T DO fimngL) 1400 mg NaCVL Conductivity (;L ) Sooo g P WS.

TiI Sq I

7( 7.o4 Z5l Temperature 1C) I 24.¶1 I Z11.9 I STOCK Conductivit I 1 JI D (Ig m hos/cin _I 000 MWE _niIa Ial Ini ial I I itial - I -- F i -al l- -Ii ti-a Ij Final I

Environmental Testing Solutions, Inc. Page 6 of 6 Enirnena TetnouinIc ae6o Species: Ceriodaphnia dubia CdNaCLCR M 2: Analyst Ana __&4. Concentration Parameter pH (S.U.) 13-12 gi7l It Z.2A# DO (mgL) l 8.0 6 I CONTROL Conductivity A 9 O Alkalinity Hlardness 'Temperature .pHI (S.U. 909<1 "o o 8 e.as exit 1Bo DO (mg/L) e B Z.z -L 6So 600 mg NaCVL Conductivity T scmpe )tr 1ZS. MO1 2-co

w Temperature X.

b 2 2.O 75.0 tt g pE (SU)ZS0Q f 1 3t .D 8.1 31 0 q.q11 1q~ IpH e i7-ex DO (mgL)8. I 800 mg NaCUL ConductiityZ Temperature 25 ?A.6 2'(.1 2S.0 I25. Z 1 2q! pH (S.U.) I-4 3jJ -___1 DO (mg/L) ___8~.

6.

8 g1000 mg NaCVfL Conductivity Temperature 2.S 1z4i 2l1 24 7.I L. MS 2.5 .ca.- 2-6 -+ Z. -;2. 1200 mg NaCIL pH (S.U.) 5.ln BZ49 I 8so.. I 5.1 I ggz~ I I.' -7J'16 I DO (mg/L) Conductivity (pmhos/cm) Temperature (00C ZS. O .J9 zwoo MM 7q.-I I zq.j 4A 25- _8 6.1 8 sl

2. q1 I 2qt I 1.0 I

I I r Big (S.U.) 5-a-I QrLn I q.o7 I f.10 1400 mg NaCVL DO (mgAL) I -:;.e Conductivity (sLmhos/cm) Temperature CC) zoqc 2q. L q t-7 ZQ . Final _ Inltial Fl 2q. 4 2.q zq.(a A I ~OK Conucivity1 Initial I I Initial I Flail I IIitia

PERMITTEE NAME/ADDRESS (Incude FecilfvNarnieocat8on Dfflm Name TVA - SEQUOYAH NUCLEAR PLANT Address P.Q. Bo XO200 0 yQ mmlifSY T 37384 7---

d _ _ _AHNUL -&

3 A84 pdjhf_ _MA -SEgQX,5jH tXARO___T i Q_&51UOCU NATIONAL POLLUTANT DISCHARGE ELMNATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DURJ (SUBR 01) TN0026450 101 Y I F - FINAL I PERMIT NUMBER I DISCHARGE NUMBER ANNUAL MONITORING (PCBS) l MONIRING,PERIp l EFFLUENT Form Approved. OMB No. 2040-0004 ATTN: Stephanie A. Howard From 03 08 l01 To l 04 l Q 7l 31 l NO DISCHARGE [ I nuOIE: Read hisftucfton befrm co" NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER.4 J. Randy Douet Site Wice President I Cmf und Puy cil ahmWadalleStedvnuw

t.

_er I _dermW dirswoor supmviom hI a w~h a yslsm deftd as t d perm pmp" r aend mW vluata Vi titon Kubmgtd Ban mvy Iqtft of en pato or 4 4wtl PW5 who mwmp fi SYMM o ion PWWW *5tIyY re for sph Oe n Principal Environmental Engineer Wa.f Il dt.W U is,toI boo of nrW awfwldge vfWd bed Mm8, mat, aid comnp. Imae Vioitaagrica penles rfor abmhfiW SIGNATURE OF PRINCIPAL EXECUTIV k i ew rdngpoasIbid fawyidhspd-bl brl ba OFFICER OR AUTHlOR17Fn AGE-NT 4, TYPED OR PRINTED I LNIYIMNT IANU LAELANATIIN C)F ANT VIOLATIONS (RleMencq. aftacmden hqM) I jIs J r u w_ ma an use Page 1 o._1 EPA FOrmll 33Z01 lRE-V 3m} "Umsus eamons may De tlse Page I of I

PERM TFEE NAME/ADDRESS (Inchlde FslfNrrmedocaln if Diffeent Name -A SEQUOYAH NUCLEAR PLANT Address E.Q )C290 _ __ IfNROFM1E SSB-E C -AS - TN -7e Laty.... Y -_ASEQJJ OAH NUCLEA E3L8j Loca__ HAMIL TOCQUNY ___ ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAMOR DIRCHARGE MONITORING REPORT IDMR_ Form Approved. nunt I- -n (SUBR01) JMOr TN026450 103 G F - FINAL I PERMIT NUM E [CHARGENMBER LOW VOL. WASTE TREATMENT POND TV. Suuw ff ffi l RIai~rrf%0I1f1 oco~lfn l EFFLUENT I rull1 nn vL From T 04 o 07 l 01 l NO DISCHARGE [ 0 NOTE Read Insuctn befo complet this forrm PARAMIETEiR QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.0 8.7 12 O 13131 GRAB MEASUREMENT 000

7~a

~~OY> SU ~ FIER ~T EFFLUENT GROSS VALUE SU E SOUDS, TOTAL SUSPENDED SASPLE 159 219 25 16 19 19 0 4131 GRAB 00530 1 0 0 LSD ~ 711 EFFLUENT GROSS VALUE LBS/DY M GIL JX OILAND GREASE SAMPLE <48 <58 26 <5 <5 19 O 4131 GRAB 00556 1 0 0 LDS/DY MGIL '7 MM< 7m T' EFFLUENT GROSS VALUE 6 M VT DI~iX hA~ IbAILM~ FLOW, IN CONDUIT OR THRU SAMPLE 1201900 ~ 311 TTL TREATMENTPLANT MEASUREMENT 1.230 1.950 03 31/31 TOTALZ 50050 1 0 0 ROMGD T 0 A f OYM NGROSS VALUE SAMPLE IMMAUREMENT MEASUREMENT CT 7II7EM7NT SAMPLE MEASUREMENT PLE

RMT, NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Cd_ todr Panaly of low tt tNis domd dard

A aad-w.

Vepd Urrmy Ku TELEPHONE DATE d adlo-Nor srisim n hi adi ut, wi a systm designd to *s*a wstt qualfwd pwrarVn nW~~)< J. Randy Douet PPIY gatherandevaluatthefWttan e b Baed enmyhdf taepon or hk Vi Preton ae n 1ronneurn -mis.* to b s od my ofbowerdgs awid Wet, Mm, Principal Environmental Enghieer 423 843-8700 04 08 12 ade V -n a, l e m s1 t dpentesfor lftfaie SIGNATURE OF PRINCIPAL EXECUTIVE J TYPED OR PRINTED hgipoq d tO. Imd hr b r J OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Rernc. ad tachmnts here) EPA Form 3320-1 (REV 31) Ptyus Pufs mayS bue Page I of I

PERMITTEE NAME/ADDRESS (Induds FacilitvNameocatn IDfDilerent Nams TA - SEQUOYAH NUCLEAR PLANT Addres PO B) 00XJOO_ _ _ __ tfEROLF CE S- -2B - ____ODDY-DAISY__JN 3738L Loclon _AMILTO COU ______NTY_ NATIONAL POLUUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 [ TN0026450 107 G F - FINAL PERMIT NUMBER DISCH GE NUMBER METAL CLEANING WASTE POND 1 "nmrIT>ZImNNf nermlon I EFFLUENT IV"- Iullnp=u YEAR I MO I DAY l YEAR MO D NO DISCHARGE [M I NOTE-Read Ihstdlons before comnotina tIs form. ATrN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SATMIPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS pH SAMPLE 1 MEASUREMENT 12 000 0 0 SUuMN1 ~~ "~ EFFLUENT GROSSVALUE M U z

PHOSHORU, TOAL (S P)SAMPLE PHOSPHORUS, TOTAL (AS P)

MEASUREMENT 19 00665 1 0 0 MGIL EFFLUENT GROSS VALUE MGL SOUDS, TOTAL SUSPENDED SAMPLE 19 00530 1 0 0 3/4~ e*** MOS..iL; OIL AND GREASE SAMPLE MEASUREMENT 19 -P

- l*E**

i*n** MGL 00555 1 0 0 z~UIEE1 AALM>___ EFFLUENT GROSS VALUE M COPPERA TOTAL (ASCU) SAMPLE i19 MEASUREMENT 1 0104 1 0 0 MGII /2TL EFFLUENT GROSS VALUE

_: d D IL MGQ _; IRON, TOTAL (AS FE)

SAMPLEN T 19 MEASUREMENT 1 01045 1 0 0 MOIL~; ss EFFLUENT GROSS VALUE R = MG/L ¶ b V FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MESUREMENT 0 50050 1 0 0 F R MI'O"1T MGDcmii77 a EFFLUENT GROSS VALUE MGD m NAMEmflTLE PRINCIPAL EXECUTIVE OFFICER I Cwu nder pmiIy d owf w M docum d t m rerd wI y lI TELEPHONE DATE dieciton orsupwisimionehwhIN codith.system degned to semw qt dpt ,lTELEP HONE DAn J. Randy Douet sgr ed e n b Bed on my nqu dof w peon or k, hO d b ofti055 pifI md' diw w beK Principal Environmental Engineer 423 843-6700 04 08 12 Site Vice Poesident amcm I ws e gnt pre fr dxug b e SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED ar-xn. khdard hw pmalfofflmipd ht i W bri b OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VK)LATIONS (Reabtw aD atachments hem) No Discharge this Period EPA Form 3320-1 (REV 3/99) Phwiu edflom may be used Page I of I

PERMITTEE NAME/ADDRESS (ncude Fei#tvNinmocatnI HDifferent) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _ PEN ERF IXSdLL&A S__ ODOY - DAISY_ Th3_ Fadjly_ _MA-SE ,AH NUCLEAfl Tj Locton HAMILTO_CLN QUY NATIONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (R) (SUBR 01) I TN0026450 110 G F - FINAL I PERMIT NUMBER I ISCHARGE NUMBER I RECYCLED COOLING WATER l-_ _,_MONIRING ERIO EFFLUENT Form Apprioved. OMB No. 2040-0004 ATTN: Stephanie A. Howard YEAR I LMOf IDAY oI From i04 l07 O1 l ITo 04 l 07 l 311 NO DISCHARGE [] NOTE Read Irtsbuclons beore Oomolelhis toftnm PARAMETER QUANTITY OR LOADING QUALTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS pH SAMPLE MEASUREMENT 12 000 I 0 0 ~ ~Su EFFLUENT GROSS VALUE U m INmuJ -A-SOUDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 0 MCIIIMN1L~~,h~ EFFLUENTGROSS VALUE t z OIL AND GREASE SAMPLE 19 MEASUREMENT 19 00556 1 0 0 MGIL ~ 1AL~e o EFFLUENT GROSS VALUE MGIL COPPER, TOTAL (AS CU) SAMPLE .*aw*. MEASUREMENT 19 01042 1 0 0 ¶' MI. F EFFLUENT GROSS VALUE E~tIZEM IRON, TOTAL (AS FE) SAMPLE DAL-X MEASUREMENT 19 01045 1 0 0 ~' I-~~*¶ EFFLUENT GROSS VALUE MG/L 7 PHOSPHORUS, TOTAL (AS P) SAMPLE

* *n*

19 MEASUREMENT 19 00555 1 0 0 EFFLUENT GROSS VALUE T7 Y MG/L A FLOW, IN CONDUIT OR THRU SAMPLE03a TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 PERMGD U EFFLUENT GROSS VALUE MAG.AIY X NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER I C ypealtoatIsdo md a reped d tdrmy TELEPHONEDT rh nndm s do ttinswqualfedporl TELEPHONE J. Randy Douet utsr - svs himsln bmtled sW mo InqukyOf d poncr or Site Vice President tWi I lo~lmbwran~dp~b" Puincipal Environmental Engineer 423 843-8700 04 08 12 ~ sidom!tee.I m e Mst hee m sg~lflmwpealtesf~ ukltlngfase SIGNATURE OF PRINCIPAL EXECUTIVE 4JL TYPED OR PRINTED thk poesslly odf finW nd htonwtfbr nwhigft OFFICER OR AUTHORIZED AGENT A EAl NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reemch aIachnents here) No Discharge this Period FA F hrm yFV 41D Pk... urn rue *n bout W ton v ad rrwtmffnnn lay Lo Usev Page 1 of I

PERMITTEE NAMEIADDRESS i7ncd FUclitV Nactln fDiffent) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O.BOX 2000 SODDY - DAISY_ T 37384 _d_ _VA E L. _tlp M LTON OsU _kjLXv_________ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) TN0026450 116 G F - FINAL I PERMIT NUMBER I I DISCHARGE NUMBER BACKWASH l MONITORING,PERIO EFFLUENT Form Approved. OMB No. 2040-0004 From IDAY l F MO From 04 107 1 J1 To 04 107 31 I NO DISCHARGE = NOTE! Rand khsteMns befam ammOMUM this brnn. ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS I DEBRIS, FLOATING (SEVERITY) SAMPLE 9A 0 1/31 VISUAL MEASUREMENT 01345 1 0 0 pq 1.iPASSNO IUI EFFLUENT GROSS VALUE FAILmI OIL AND GREASE VISUAL SAMPLE O 31 VISUAL MEASUREMENT 84086 1 0 0 RT YES-1 M7-i -4EE EFFLUENT GROSS VALUE N_-_ O z SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT NAMEmTLE PRINCIPAL EXECUTIVE OFFICER CuIf du petdy o w dond m ettmntw pu d uad mny 1 TELEPHONE DATE drcton or q ohawam In c-r lo Wfhaaydmo e toueVWqmIfedd b d pA .rs nAQJcl J. Randy Douet pgtr id aate ftliun atbuited easd on my kuqry of toh pomn or M Site Voft hb mbt~d myIoftbbodpWanm d b~ef, VPrincipal Environmental Engineer 423 843-6700 04 08 12 'S Vnt acuade md c

t. I mo mea ee me slgdi pats forb behr n

SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED Ihh. m',kck eftpossbftq db"*pi=. b rlobwrhg visbi OFFICER OR AUTHORIZED AGENT EA NMER YEAR MO DA COMMENTS AND EXPLANATION OF ANY VIOLATIONS ftawmqff d athments here) Operations performs visual Inspections for floatIng debris and oil and grease during an backwashes. EP Fr _RE ,19 i edti. e u. e Pae1o EPA Fon. 3320.1 (REV 31") Pmvfous edftns may be used Page I of I

PERMITTEE NAMEIADDRESS (Incude fault Narnmeocfton fDff~wert Name __A - SEQUOYAH NUCLEAR PLANT ____ AAdAsL__E £Q:2)C2000 ____(INU&ERFICE SB-20 ___j SQDY AIS_ Nj~tw Bca~on _AMILTONCQ U_______ ATTN: Stephan A. Howard NATIONAL POLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT MUM (SUBR(01) LI TN026450 117 G F-FINAL I PERMITNUMBER I ISCHARGE NUMBERl BACKWASH I MONITORING PERIOD I EFFLUENT Form Approved. OMB No. 2040-0004 YFARro MO I DU A YEAR L LMO DA From 04 T 07 T 01 I To 04 1 7 13i1 NO DISCHARGE GE NOTE: Rea Inshictim before completina this Ibrm PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANA YSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A O 1 /31 VISUAL MEASUREMENT 01345 1 0 0 ,r~REQ* JIR MEWN 77 A YMO 7 i A7. FAIL-I0 EFFLUENT GROSS VALUE FAI_1 M OIL AND GREASE VISUAL SAMPLE 0 e***1131 VISUAL MEASUREMENT 84065 1 0 0

G 7i YESI

= EFFLUENT GROSS VALUE TNOR SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT ~ NAMEtlTILE PRINCIPAL EXECUTIVE OFFICER ICly wer pey or p W y bout docanmiid satad we' er wpddr my IE TELEPHONE DATE dbeon or superYin mi do wih a systav designed to eaxmm t qimtlfed peruae = -g-I J. Randy Douet pnledY g9her mid vahutetImion.bmft d on y k*y od Ie peron or peSrAnite VWie P U ids tnUmwme b dmy perne qapond e, h PrincIpal Environmental Engineer 423 843-8700 04 08 12 t~nwtn.Vekftomutko Wmhite Ia. to do. bedat nrm lmaldg NW beter true 423__843_6700__04____12 SVi.res and a.nqW I cm I mn soon p M thi wen algriwfcmg buetx d n f' SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED hdIM h p d h OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (1me al atcmenb hee) Operations peforrns visual Inspections for floating debris and oil and grease during aD backwashes. EPA Foffn 3320-1 (REV 31") PYVVIM edmons may be USW Page I of I

PERMITTEE NAME/ADDRESS llnduds Fad7 Nva4ocon fDffIeren) Name TVA - SEQUOYAH NUCLEAR PLANT AddK LP.O_. QBOX20W ____________ SODDY-DAIY TN 73e4 fldft _[VX -SE2jJOYAHN UCEAR PLANT ATTN: Stephanie A. Howard NATIONAL POLUTANT DISCHARGE ELMINATION SYSTEM (NPDES) MAJOR I DISCHARGE MONITORING REPORT (DMRU (SUBR 01)C TN0026450 118 G F-FINAL PRMIT NUMER I DISCHARGE NUMBER WASTEWATER & STORM WATER I MNITORING FRI I_ EFFLUENT Form Approved. OM8 No. 2040-0004 YEomTMO YER l From 04 107 Q1 01 To 04 107 131 NO DISCHARGE [ 1 NOTE: Read bIstnucion befr complena this bnnm. NAMEnTLE PRINCIPAL EXECUTIVE OFFICER I Cayudrpeayof lwia

downd alt diwswemwd td my lI TELEPHONE DATE Jrdeeftcrsuipwvlsn bi a

-dencwlfi asytem dahlgradto aswe tatq~miffed pu'aramm AQaAdAm-M J. Randy Douet p s r w oakni ft blannebn nibittb Bda an Irq hquiry df b Pam or J Site VRne President k O h _a __o'g b ",h b Principal Environmental Engineer 423 843-700 04 08 12 sos, wnd ax. I ewrmetereme sla peneforSbblttlh fse SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED h dh g poftltoy df few end hiir lsonl fbrv5kl5a. OFFICER OR AUTHORIZED AGENT EA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reftrence olf uffachnmens hee) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfHl. EPA rwmi JJU1-1 trltV JI toFuVsID ewwons may De used Page 1 of 1}}

ML042290246

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