ML041700165
| ML041700165 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 06/09/2004 |
| From: | Howard S Tennessee Valley Authority |
| To: | Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML041700165 (118) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 June 9, 2004 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MAY 2004 Enclosed is the May 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission If E6S ATTN: Document Control Desk Washington, D.C. 20555 Prnted On l nycd pa
PERMITTEE NAMEIADDRESS (Include Facility Name/LocatIon if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
_ _ _ _L1? E~ROF9CqE SB-M
)_
____- SODDY - DAISY _
TN 378 4 adj!y_ TVA - SEQUOYAH NUCLEAR PLANT__
Location HAMILTON COUNTY--
ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
-64 OF
-FINAL l TN0026450 101 G FINL I
PERMIT NUMBE DISCHARGE NUMBER I DIFFUSER DISCHARGE I
MONITOPRING. PERIODl I EFFLUENT Form Approved.
OMB No. 2040-0004 YEAR I MO I DAYI YEAR I MOl DAY From 04 l 05 l 01 I To 04 l 05 l 31 l
'* NO DISCHARGE 0
NOTE: Read Instructions before completina this form.
I I
t I
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Ceutify under penalty of law that this doijment and all attachments were prepared under my TELEPHONE DATE J. Randy Douet
-raction or supervision in accordance with a system designed to assure that qualified personnel L i -I property gather and evaluate the information submitted. Based on my Iquiry of the person or
.A a
a tJ persons who manage the system or those persons directly responsible for gathering t Principal Environmental Engineer 423 843-6700 04 06 10 Site Vice President nfomaffon.
the Information submitted is to the best of my knowledge and belief, true I4 eaurate and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information. induding the possibility of fine and Imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
___IDE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference atl attachments here)
No dosed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.060mg/L-limit 0.100mg/L) 2. CL-363 (max. calc. conc. was 0.01 1mg/L-limit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.021 mg/L-limit 0.1 00mg/L) 4. H-1 30M (max. cal. conc. was 0.026mg/L-limit 0.050mg/L) 5. H-1 30M (low detection level analytical method was <0.020mg/L-limit 0.050mg/L))
E P F o r 3 3 0.
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.a e o 2 EPA Fortn 3320-1 (REV 3199)
Previous edlillons may be used Page I of 2
PERMITTEE NAME/ADDRESS
{Include FacilityNameAocation if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ____________
_NEOFC 2_ _ _ __
Faoy__A -SEOUYAH NUCLEAR PANT___
LocaEqon _AMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN026450 101 G F-FINAL PERMIT NUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE I
MONITORING PERIOD l
EFFLUENT Form Approved.
OMB No. 2040-0004 IY IMO I DAY I AR MO DAY From 04 05 1 01 I To 04 105 j
311 NO DISCHARGE
=
NOTE: Read Instructions before completina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE
_EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE -C, RATE OF SAMPLE 0.1 62 0
31 /31 CALCTD CHANGE MEASUREMENT 82234 1
0 0
-- 'ERI-e.*e*-7.
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,~
'01.0 DEC
- CONTIN, 'CALCTD.
EFFLUENT GROSS VALUE 1REO1JIRMTHR
- .; UOUSa*
TEMP. DIFF. BETWEEN SAMP. &
SAMPLE 2.2 04 0
31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1
S 0
_E X
3.0 j
DEG.JC.j X
C EFFLUENT GROS VAUuEUREEO
,U'~~
-- ~
BORON, TOTAL SAMPLE
<0.2
<0.2
<0.2 19 0
1/31 GRAB MEASUREMENT 01022 1
0 0
.MIL OREPi MGL m
SAMPLE MEASUREMENT A
a A
SAMPLE MEASUREMENT PER I`17,e-A.-.-
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SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT-P-
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I.*.
REQUIREMET.~..~-..--
NAMEMTLE PRINCIPAL EXECUTIVE OFFICER I Ce&ify under penalty of law that this docjment and all attadlments were prepared under my TELEPHONE DATE direction or supervision in accordanc with a systemX designed to assure that qualified personnel J
t l
o sP J. Randy Douet property gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system or thse persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 04 06 10 Site Vice President information, the Infonimatlon submitted is, to fth best of my knowledg and belief. two, j
accurate. and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTED information. Including the possibility of ine and Imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT E
NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)
CCW data for May 2004 Is attached.
EPA Form 3320-1 (REV 31991 Previous editions may be used Page 2 of 2
CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 5/5/2004 1020
< 0.5 mg/L 516/2004 1926 CVS EPA 8015B CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis DatelTime Analyst Method 5/5/2004 1015
< 0.5 mg/L 5/6/2004 1849 CVS EPA 8015B
PERMITTEE NAME/ADDRESS (Indude Facilit NametocatIon if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT________
Address P.
BOX 2000
_[fTEEROM(CE aN-
-_-_-_SODDY
- DAISY TN 37384 fadW__ VA - SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR For DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OM I
TN0650 101 T F - FINAL PERMIT NUMBER I i DISCHARGE NUMBER IOMONITORING FOR OUTFALL 101 MONITORINGPERIOD EFFLUENT mn Approved.
[B No. 2040-0004 From 1 4105 01 ITO 0~4jp 0 1 3 NO DISCHARGE F
- 1 NOTE: Read Instructions before compleling this form.
1*
NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my t
^
11
/
TELEPHONE DATE direction or supervision in arcordance with a system designed to assure that qualified personnelI J. Randy Douet property gather and evaluate the information subrnitted. Based on my inquiry of the person or
- 4.. Wfa i i persons who manage the system. orthose persons directly responsible forgathering the Principal Environmental Engineer 423 843-6700 04 06 10 information. the Information submitted Is.* to the best of my knowledge and belief. true, 423___843___6700___04___06______
Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI YPD RP NEinformation.
Including the possibilityof fine and Imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED
_CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled May 4-9, 2004. Report is attached.
EPATorm 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
June 4, 2004 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, MAY, 2004 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected May 4-9, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cog Am Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN May 2004M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: June 4, 2004
- 1. Facility/ Discharger: Sequovah Nuclear Plant I TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDESPermit #: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. DesignFlow(MGD): 3.266
- 7. 1Q10: 2.992.4
- 8. OutfallTested: 101
- 9. Dates Sampled: May 4-9, 2004
- 10. Average Flow on Days Sampled (MGD): 1567, 1572,1573.1563.1548.1560
- 11. Pertinent Site Conditions:
H-130M molluscicide was injected from May 4-7, 2004. The dates and times for the H-130M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period.
Injection Location Date/Start Time Date/Ending Time (EDT)
(EDT)
Essential Raw Cooling Water (ERCW) 05-04-2004/0905 05-07-2004/0904 B Train
- 12. Test Dates: May 6-13, 2004
- 13. Test Type: Short-tenn Chronic Definitive
- 14. Test Species: Fathead Minnows (Pirnelihales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Intake: 100.0 Pimephales prornelas: UV treated Outfall 101: 10.98. 22.0. 43.9, 72.0. 100.0 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: IC), > 43.9%
- 17. Test Results: Outfall 101: Pimephales promelas: IC., > 100%
Ceriodaphnia dubia: IC,5 > 100%
UV treated Outfall 101: Pirnephalespromelas: IC?,> 100%
- 18. Facility
Contact:
Ruth Ann Hurt Phone #: (423) 843-6714
- 19. Consulting/Testing Lab: Environmental Testing Solutions, Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected May 4-9, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DDNY)
(MM/DD/YY)
Arrival Initial (MM/DD/Y)
Sample Time (EST)
Time (EST)
Temp.
TRC*
Time (EST)
ID Collected Received (mgL)
Used By 101 05/05/04 0643 05/05/04 1340 1.1t
<0.10 05/07/04 1426 Intake 05/04/04 0751 to 05/05/04 1340 1.0
<0.10 05106/04 1350 05/05/04 0651 05/07/04 1426 101 05/07/04 0630 05/07/04 1311 1.2t
<0.10 05/08/04 1443 05/06/04 07153t 05/08/04 13443 Intake 05/07/04 0615 05/07/04 1311 1.4
<0.10 05/09/04 1340 0510804 086 to05/10/04 1356 101 05/09/04 0746to 05/09/04 1335 3.Ot
<0.10 05/11/04 1340 05/0/04 74605/12/04 1349 05/0804 089 to05/10/04 1356 Intake 05/08/04 0859 to 05/09/04 1335 3.5
<0.10 05/11/04 1340 0000 05/12/04 1349
- TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers.
cubitainer upon arrival.
Temperature was measured in each
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- L.0MC) in a warm water bath.
Aliauots of Outfall 101 and Intake samnles were UV-treated through a 40-watt Smart V Sterilizer (manufactured bv Emperor Aquatics, Inc.) for 2 minutes.
3
Pimephales promelas Ceriodaphnia dubia Test Organisms:
- 1.
Source:
Aquatic BioSystems, Inc.
In-house Cultures
- 2. Age:
22.0-24.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old
<24-hours old Test Method Summary:
- 1. Test Conditions:
Static, Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. OrganismsperReplicate: 10 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101 05/06/04 - 1350 EDT 05/06/04 - 1334 EDT 05/13/04 - 1322 EDT 05/13/04 - 1345 EDT 05/06/04 - 1123 EDT 05/13/04-1100 EDT
- 8. TestTemperature: Outfall 101:
Mean=24.60C (24.0 - 25.40C)
Test Temperature: UV-Treated Outfall 101:
Mean = 24.90C (24.2 - 25.40C)
Mean = 24.70C (24.1 - 25.40C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelns Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 6 - 13. 2004 using effluent from Outfall 101.
Test
. Percent Surviving Solutions (time interval used - da ys)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Intake 100 100 98 95 95 95 95 Test Solutions Mean Dry Weight (mg)
(% Effluent)
(replicate number)
Mean 2_ _
3 4
M ean Control 0.867 0.915 0.837 0.858 0.869 10.98%
0.825 0.898 0.958 0.786 0.867 22.0%
0.950 0.831 0.724 0.821 0.832 43.9%
0.764 0.819 0.942 0.694 0.805 72.0%
0.920 0.893 0.950 0.804 0.892 100%
0.822 0.875 0.936 0.947 0.895 Intake 0.973 0.770 0.922 0.954 0.905 IC25 Value: > I 00%
Permit Limit 43.9%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Permit Limit= 2.3 TUe Lower Limit: NA
- TUa = 100/LC 5o: TUc = 100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 6 - 13. 2004 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 1
2 3
4 5
6 7
Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 100 100 90 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100
.100 100 100%
100 100 100 100 100 100 100 T lReproduction
(#young/female/7 days) est Solutons Data (replicate number) 1 2
3 4
5 6
7 8 l 9 10 Mean Control 27 27 33 32 28 32 31 32 28 32 30.2 10.98%
32 32 30 35 32 31 31 32 36 30 32.1 22.0%
36 31 29 36 35 33 32 32 38 30 33.2 43.9%
35 34 34 39 33 33 36 32 37 32 34.5 72.0%
37 33 34 36
.36 36 36 32 39 32 35.1 100%
36 36 35 37 36 39 39 33 38 35 36.4 IC25 Value: > 100%
Permit Limit= 43.9%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Permit Limit= 2.3 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/ IC25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 6 - 13, 2004 using water from Intake Percent Surviving Test (time interval used - days)
(%Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(% Effluent)
Data (replicate number) 2 l3 l4 5
6 l 7 8
9 10 Mean Control 30 32 32 29 28 35 31 36 28 36 31.7 Intake 28 31 30 31 35 30 34 34 29 I32 31 IC25 Value: > 100%
Permit Limit: N/A Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Permit Limit: N/A Upper Limit: NA Lower Limit: NA
- TUa = 1 00/LCso: TUc = 100/ IC25 7
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted May 6 - 13, 2004 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22.0%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72.0%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Intake 100I 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)
(% Effluent) 1 2
(replicate numb er)
Mean 1__
23 4
M ean Control 0.877 0.752 0.933 0.874 0.859 10.98%
0.824 0.693 0.856 0.837 0.803 22.0%
0.737 0.818 0.971 0.876 0.851 43.9%
0.851 0.827 0.820 0.897 0.849 72.0%
0.965 0.975 0.938 0.918 0.949 100%
0.992 0.959 0.755 0.927 0.908 Intake 0.896 0.879 0.952 0.938 0.916 IC25 Value: > 100%
95% Confidence Limits:
Calculated TU Estimates: < 1.0 TUc*
Upper Limit: NA Lower Limit: NA
- TUa = 1OO/LC5o: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25)
Pirnephales pronielas May 6 - 13, 2004 1419 7-days KCI 0.63 g&L Ceriodahtnia dubia May 4 - 11, 2004 1355 7-days NaCl 1.07 g/L 8
PHYSICAlUCIIFNIICAL SUNtMARY Water Chemistry Mean Values and Ranges forPirnephatespromelas and Ceriodapinia dubia Tests, Sequoyah NuclearPlant Effluent (SQN), Outfall 101, May6-13,2004.
Test Sample ID Temperature CC)
Dissolved O ygen (mgtL) p1l IS.U.)
Conductance Alkalinity Hardness
- Total Residual Initial Final Initial Final Initial Final (pmhosrcm) (mglL CaCO3) (mgtL CaCO3 ) Chlorine (mg/L)
Control 24.1 24.2 8.1 7.8 7.76 7.45 304 62 82 24.8 - 25.3 24.1 - 24.4 7.9 -
8.3 7.3 -
8.2 7.49 8.05 7.25 - 7.55 287 - 331 60 64 82 82 10.98%
25.0 24.2 8.1 7.8 7.64 7.44 288 24.9 - 25.2 24.1 - 24.5 7.9 -
8.3 7.3 -
8.2 7.52 - 7.76 7.26 - 7.53 273 -
300 25.1 24.2 8.1 7.8 7.62 7.43 278 E
24.9 - 25.2 24.0 - 24.3 8.0 -
8.2 7.3 -
8.1 7.48 - 7.74 7.26 - 7.52 264 - 288 Q
43.9%
25.1 24.3 8.1 7.7 7.58 7.42 250
- 3.
24.8 - 25.4 24.1 - 24.4 7.9 -
8.3 7.3 -
8.2 7.44 - 7.72 7.26 - 7.48 239 - 262 72%
25.1 24.2 8.2 7.8 7.55 7.42 218 7
24.6 - 25.4 24.1 - 24.4 8.0 -
8.3 7.4 -
8.1 7.38 - 7.71 7.28 -
7.52 207 -
226 100%
25.1 24.2 8.2 7.8 7.52 7.45 186 67 72
<0.10 24.5 - 25.4 24.1 - 24.3 7.9
- 8.3 7.4 -
8.1 7.35 - 7.69 7.27 - 7.56 173 -
193 66 69 71 75
<0.10 -
<0.10 25.0 24.2 8.3 7.8 7.64 7A9 185 64 73
< 0.10 Intake 24.8 - 25.2 24.1 - 24.3 8.0.
8.4 7.5 -
8.1 7.61 - 7.71 7.37 - 7.57 178 -
191 60 67 71 75
<0.10 -
<0.10 Control 25.1 24.9 8.1 8.1 7.76 7.63 304 62 82 Contro 24.8 - 25.4 24.4 - 25.2 7.9 -
8.3 8.0 8.2 7A9 - 8.05 7.49 - 7.69 287 -
331 60 64 82 82 10.98%
25.0 24.9 8.1 8.2 7.64 7.63 288 24.7 - 25.4 24.3 - 25.2 7.9.
8.3 8.0 -
8.2 7.52 - 7.76 7.52 - 7.69 273 - 300 22 25.0 24.9 8.1 8.2 7.62 7.62 278
__22/
24.7 - 25.2 24.3 - 25.1 8.0 -
8.2 8.0 -
8.3 7.48 - 7.74 7.48 - 7.69 264 - 288 24.9 24.9 8.1 8.2 7.58 7.60 250 24.5 - 25.2 24.4 - 25.2 7.9
- 8.3 8.0 -
8.3 7.44 - 7.72 7.40 - 7.68 239 - 262 72%
24.9 24.8 8.2 8.2 7.55 7.60 218 24A - 25.2 24.2 - 25.1 8.0 -
8.3 7.8 R.4 7.3R 8 7.71 7.38 - 7.66 207 - 226 100%
24.9 24.9 8.2 8.2 7.52 7.59 186 67 72
< 0.10 24.3 - 25.24.4.4
- 25.1 7.9 8.3 7.9 8.5 7.35 -
7.69 7.37 -
7.66 173 -
193 66 69 71 75
<0.10 *
<0.10 Intake 25.0 24.9 8.3 8.2 7.64 7.59 185 64 73
<0.10
_ _24.7 - 25.4 24.4 - 25.1 8.0 - 8.4 8.0 -
8.4 7.61 - 7.71 7.36 - 7.68 178 -
191 60 67 71 75
<0.10 -
<0.10 Overall temperature (C)
Pimephlaes prornelas Ceriodaphnia dubla Average 24.6 24.9 Minimum 24.0 24.2 Maximum 25.4 25.4 9
- PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pinieplialespronielas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, May 6 - 13, 2004.
Test Sample ID Temperature (CC)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Initial Final Initial Final Initial Final (fimhos/cm)
Control 25.1 24.3 8.1 7.7 7.65 7.47 289 24.8 - 25.4 24.1 - 24.5 7.9 -
8.2 7.4 -
7.9 7.49 - 7.85 7.31 - 7.66 277. -
303 10.98%
25.1 24.3 8.1 7.7 7.64 7.47 287 24.9 -
25.4 24.1 - 24.5 7.9 8.2 7.5 8.0 7.50 -
7.85 7.34 - 7.66 267 -
301 22%
25.0 24.3 8.1 7.7 7.63 7.47 271 24.9 - 25.2 24.2 - 24.5 8.0 -
8.2 7.5 8.0 7.49 -
7.83 7.39 - 7.67 252 - 287 25.0 24.3 8.1 7.7 7.61
.7.47 252 3
24.9 - 25.3 24.1 - 24.5
. 8.0 -
8.3 7.5 8.0 7.47 - 7.81 7.36 - 7.64 241 -
263 72%
25.0 24.3 8.2 7.7 7.60 7.48 219 7
24.9 -
25.3 24.1 - 24.5 8.0 -
8.3 7.4 -
8.0 7.44 - 7.79 7.40 - 7.60 208 -
228 100%
25.1 24.3 8.1 7.7 7.57 7.47 186 124.9 - 25.4 24.1 - 24.5 8.0 8.3 7.6 -
8.0 7.40 -
7.76 7.38 - 7.61 177 -
195 Intake 25.1 24.3 8.2 7.8 7.64 7.51 185 24.9 - 25.4 24.1 - 24.5 7.9 -
8.4 7.4 -
8.2 7.54 - 7.78 7.44 - 7.62 178 -
191 Overall temperature CC)
Pinep hales promelas Average 24.7 Minimum 24.1 Maximum 25.4 10
. I
. ' -- 7.%
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected May 4 - 9, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms. These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pirnephales promelas None
- 2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pintephales prornelas None
- 2. Ceriodaphnia dubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Total Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturinglacquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Anyknown deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KCI or NaCI.
- 2.
Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.
Sodium Chloride (NaCi crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
14
Sequoyah Nuclear Plant Biomonitoring May 6 -13, 2004 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling,
Table B-i. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-May 9, 2004 2
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-May 9, 2004 Date Sodium Hypochlorite mg/L, - :,
-} '
Towverbrom mg/L TRC PCL-222 mg/L Phosphate, PCL-401 mg/L Copolymer CL-363 mgAD.;
DMAD,
Cuprostat-PF mgtL Azole H-130M mg/Li Quaty'
.,-.1 'R 4
4 4--4 S
01/31/2000 02/01/2000 02/02/2000 02/03/2000 02/04/2000 02/05/2000 02/06/2000
' S
'u;R, XI 4x~,
< 0.002 0.011 0.028 0.008 0.006
< 0.002
< 0.002 0.026, 0.026. e, 0.026 0.06~
0.027,..
0.027 t 0;027 ^
0.027.;
0.009 0.028 0.009 0.009 0.009 0.009 0.009 0.
0.
0.109 07/26/2000 07/27/2000 07/28/2000 07/29/2000 07/30/2000 07/31/2000 08/0112000
< 0.0057 0.019 0.0088
< 0.0088
< 0.0076
< 0.0152
< 0.0141 0.055; 0.055; 0.055; 0.055 - :,
0.055 ' '
0.019 0.019 0.018 0.019 0.019 0.019 0.019 7.,.
- 1.:
0.004 '3/4 0.006,X 0.005 0.108 12/11/2000
- t 0.0143 0.025 0.020 0.005 r 12/12/2000.,.
sU-0.0092 0.025 0.020 0.005 12/13/2000
< 0.0120 00.025' 0.020 12/1412000
< 0.0087 0.025,'
0.020 12/15/2000 0.0120 0.025 0.020 0.005':
12/16/2000
< 0.0036 0.025 0.020 12117/2000
< 0.0036 0.025 -'
0.020 08/26/2001 0.017 0.06,.,
0.021 0.006-08/27/2001
<0.0096 0.06 0.021 0 005 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059.
0.020 0.005 4 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001
<0.005 0.059.
0.020 11/25/2001
<0.0044 11/26/2001
<0.0119 0.024 0.02 0.005'$`
11/27/2001
-0.0089 0.0232 0.019 0.006 11/28/2001
<0.0089 0.022 0.019 0.006 !
11/29/2001 0.0132 0.024 0.02 0.007 3-1 11/30/2001 1
< 0.0043 0.024 0.02
)-
12/09/2001 ;
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128 0.024 0.02 0.008 '
12/13/2001
<O.OOS8 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 3
Table B-i (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-May 9, 2004 Date Sodium Hypochlorite '
-ng/L:,:
-TRC O Towerbrom mg/L TRC PCL-222.:
mg/Lht&
Phosphate '-
I PCL-401.
mg/L Copolymer
- CL-363.,
mgIL~-f DMAD Cuprostat-PF mgfL Azole 01/02/2002
< 0.0079 0.023 0.02 0.006tj 01/03/2002
< 0.0042 0.023.
0.014
,rq 02104/2002 0.0124 0.024 0.014 0,007,
01/05/2002 '
< 0.0042 0
8, 01/06/2002,,
<0,0042 0
0 01/07/2002 1
<0008 0.024 0.02 0.0 050/20 0.00-9
-0.5;
.014 06at4; 02/24/2002
<0 0004 0225/12002
< 0.004 0.053;~
0.023 02/26/2002
- 0.
00143 0.023 ::;
0.023 0.0 02/27/2002
<0,0041 0.023,;.'
0.023 02128/2002
<00041 0.05 4
0.008 03/01/2002
-:1;- ;.
<0,004 0.025-'
0.00819
.007 05/06/2002 0.058 5
0.02 0.014 :
05/07/2002.
0.0584'-
0.02 0.015 05/08/2002 0.. 0 0.0 19 9
05/09/2002
<,4 0.05i 0.02 0 01 101/02;;,,
.14 0.05i,.- 1 0.0182 o.0o..
05/110/2002
<0.0042 0.054 2
0.019 08/04/2002 -
<0.0058 08/05/2002 0.0058 0.05-3 0.018 08/06/2002 0.0092 0.053 0.018 08/07/2002
<0.0107 0.0556 0.0 19 0.007 08/0812002
<030061 0.055..-.
0.019 08/09/20021
-0;.___
<0.001.05226-o4
-.I 0.018 0'008"'
10/06/2002
<0.00497 l
.: l 10/07/2002 00153 0.0 0.018 0.009.:
10/08/2002
<00092 0.054 0.018 0.007 -,
10/09/2002 0.0124 0.053 0.018 0.009>
10/10/2002 0.0134 0.054 0.018 0.009 10/11/2002-
<0.0042 0.05'4 ':
0.018 01/12/2003
<0.0035 01/13/2003
-<0.006 0.025k 0.019 0.009 01/14/2003
<0.0118 0.026 0.020 01/15/2003
<0.0063 0.026 -
0.020 0 009 01/16/2003
-<0.0034 0.026 0.020 01/17/20031
<0.0034 0.026' 0.009___
04/06/2003
-<0.0073 04/07/2003
-<0.0189
-0.021 04/08/2003
-<0.01 17
-,0.021 04/09/2003
-<0.0139 0.021 001i6-04/10/2003
-0013-0.021 0.018
-11 04/111/2003
-<0.0073
-0.022 4
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-May 9, 2004 Date Sodium i.
Hypochlorte4.
mg/L '"
TROC 06/15/2003
< 0,0045 06/16/2003
< 00037 06/17/2003
< 0.0048 06/18/2003 0.0048 06/19/2003 0.0085 06/20/2003
-X-
< 0.0048 08/03/2003
<0.0050 08/04/2003
<0.0050 08/05/2003
.ji
<0.0051 08/06/2003.
1
<0.0084 08/07/2003 0.0129 08/08/2003 0.0153 10/05/2003
< 0y.'>
<0.0043 10/06/2003
<0.0043 10/07/2003
<0.0090 10/08/2003 s
<0.0106 10109/2003 1 r 0.0181 10110/2003 0.0183 02/01/2004 0.0093 02/02/2004
<0.0034 02/03/2004
<0.0034 02/04/2004 0.0124 02/05/2004
<0.0034 02/06/20041 0.0105 05/04/2004
<0.0123 05/05/2004
<0.0144 05/06/2004
<0.0146 05/07/2004 0.0227 05/08/2004 0.016 05/09/2004
<0.0104 5
Sequoyah Nuclear Plant Biomonitoring May 6 - 13, 2004 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Paige 11 of 3.
-Xt r1
-=4 -
7rn Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number. TN0026450 Collected By: Wanda Allen, Ronnie Hankins Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Client Other (specify): Express Courier General Comments:
Ca Voc A,
0J,
-f L.-G6 C o-dO.- Lodi Fax:
828-350-9368 Field IdentificationI Grab/Comp.
CollectionDate/Time Container Flot:ainEvent?
W/.""lW Sample Description Nuimber &
(MGD)
~..,*K.~
Volume (Mark as Appropriate) i
%XXX
_ Collected Q
Date Time A
y
- IfcYs, No TraceeS L.g lAge SQN-101-TOX Comp 54/04-5/5/04 72(2.5gal)
NA II SQN-INT-TOX Comp 5/4/04 - 5/5/04 01751 1(2.5 gal)
NA Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time Wanda Allen 5dtc 5/5/2004 Express Courier 5/5/2004 Ronnic Hankinsqe-,
5X___
____,__________At Express Couriers 9
5 5/2004 1340 ETS 5/5/2004 1 q
° xzzztzznc___
Instructions: Clients should rill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 0ZJ of 3
5
=Y-6 Client: TVA Project Name: Scquoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number TN0026450 Collected By: *Vanda Allen, Ronnie Hankins Environmental Testing Solution, Inc.
351 Depot Street Ashville, NC 28801 Phone:
828-350-9364 FedEx UPS Bus Delivered By (Circle One):
Other (specify): Express Courier General Comments:
4 c
.ADIt L-J cL.
co" o
L.--Cod di Client Fax:
828-350-9368 Field Identification /
Grab/Comp.
Collection DatefTime Container Flow Ri E t
Sample Description Nuimber &
(MGD)
Raint,.vent W;
~
Volume (Mark as Approprinte) c v x.
Collected "M'
,'.."'4.
~
Date
'Iime YKes
- Irfnd, No Trace i;Og x
3 DY~
jT>
s4 c
J nchcs
~- ~
~ '"
K w
SQN-10-TOX COmp 516104-5n104 W,50 2(2.5gQI)
NA SQN-INT-TOX Camp 5/6/04 - 517104 (2.5 Al)
NA
.,,4 4
Sample Custody - Fill In From Top Down Relinquished By (Signature):
DatetTime Received By (Signature):
Date/Time Wanda Allen A4A('t4 51n/2004 Express Courier 5/72004 Ronnie Hankins ltx~
O/.
Express Courier 5/7204 ETS 5m12004 1
'-31J Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FcdEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITOIUNG CHAIN OF CUSTODY RECORD Pager of SI DIOMONITORING CHAIN OF CUSTODY RECORD Pagejf of.31
,.a C -'M'-4%r4 I.
I Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN00264S0 Collected By: Wanda Allen, Ronnie Hankins Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
FedEx UPS Bus Client II Other (speci): Express Courier Generl Comments:
IL e
xjv L CJad-ji~~
.todClowa
&-Ipl
C&wutl,-
du--
Fax:
828-350-9368 II.
A s.
s _
Y_ s-15__
Sample LC1StoOy-VI]l In From s op Liown Relinquished By (Signature):
Date/time Received By (Signature):
Datefrime Wanda Allen IU, stn 5/9.2004 Expre>Couner 519/2004 Express Courier n
5/912004 ETS 5
3
/2004 4avA
/~1335 3
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biornonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> frorn the time of collection. Tlhrefore, please collect and ship in such a way that the laboratory will receive the samples with anple time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the Following Monday.
Environmental Testing Solutions, Inc.
Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project #:
moto Dilution prepar n information:
Comments:
Dilutionprp (%i)
I0.98 22 43.9 72 i
oo Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
Zz V
I CA.S
-zzeS.
Ot Randomizing template: l xlLun Date and times organisms Cos-,SGo4 j1SMo
'tAO ISr Incubator number:
were born between:
(tw M
o t
3 C...
Organism source:-
&amtS k GSct-cM Afrtemia lot number:
Xo Transfer bowl information:
pH Temperature =
QC Total drying time:
2q-. Z DateI Time in: Cs.v3b'_
- j Average transfer volume:
Date / Time out: bcrsA-1U3O
._9 Oven temperature:
Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used I
time termination time RAS 0
os- (..;-cA 4Z).
C os-cis -co'4
+/-zs.21
-4 1 C661 -U4 Io 1t600 b0 I17-b n4 US6 7co-S 2q
-Ak 2
M:Qtq 1
__1 ___
3 I
_6__
L o40501.0c joS.
\\
3 as-CR-L oAS'4 1
o oso 0s-N O4ioS.
O'A J.0 5 K
6 CS
.t oI IoOZc iIatO I5S c6hLI bqor.
/.oz I(EK 7Ss1z-Oq 01S; l3qj CS-il D
KEK.
7 OQ-.j3 *
-C K
ier 3 1.
__-f 1,11 I
lt iII Control information:
Acceptance ctitcria Summary of test endpoints:
1%
Mortality:
I s 20 %,
7-day LC5n
> I tq7 Average weight per initial larvae:
- a.
(l
- ',,- r NOEC Io-7 Average weight per surviving larvae:
O. UA9 2 0.25 mc/iarvae LOEC
>1 Oml ChV
> I O610 IC 15
_ __4
Environmental Testing Solutions, Inc.
Page 2 of 6 Enirnea Tetn Souios
_n.Pge2o Species: Pimephalespromelas Date:
oSGo-OA II i.
I Client: TVA I Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 10.98%
22%
_ A B
C D
E F I G H
I J
K L
10 I
l I0
/ /0 to10 bI
/
l /0
/L 1_
0 1
o 10 lb I 0 10 to 0
' to 10 10 10 2
ID 010 I)
J 1
c to oIC) 10/1010 10
}o 3
1
/1 0
/
4IC) 1
/0
/0 1/0
/0
/0 0
/0
/0o 0
/0 5
/o_
J/ 1 jA
/
/6 6/
(C A
Ia
/6
/a t
6 I t) 10
/0
/0
/0 IC
/)
/0
/0 LI)
/
1 0 1U JolD I0 10
/
/
10 IL G
A-Pan welgbt (mg)
B-Pan + Larvse welght (mge)
N\\ 7 +° M!;°5wds eE Larvae welght (mg) - A - B Ae 4%
A%
1o 0
Wtight per initial number of
- p C,
4 o p larvae (mg)
O q
O*
O*
O
-CllnlItalnumberoflarvat Average weight per Initial number oflarvae (mg)
Calculations and data reviewed:
Comments:
Environmental Testing Solutions, Inc.
Page 3 of 6 Environmental Testing Solutions, Inc.
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
aS -
-6q Survival and Growth Data Day 43.9%
72%
100%
_ l N 0
P Q
R S
T U
V I W X
0 1 lo lo lo lo 10 1o 10 /I J
t 'o 'O /
/D lOI 110 10 IO
-IO0C)
/
/
1010/
0 2
//01 /
ID
/
10 10 I
0IC
£0 o I/° 3
)O i
t 6/6 I/
0 lo) /0101 0o (a 4
to D 10 IC)
/1 1o /O 1D /
10 1O I
.5
/t
/6
/D
/O 10
/a
/)
16
/16 /
6
.C)
/
ID 100 /01-- 1I
/0/
/C) /
/ 7)0 IL)
'o lo 10 /o/
J 10101010 A - Pan weight (mg) gf :.o3D$ -
\\,,v o >,.o Ikq lt I-, vvl xb IRA
\\%( o ^
\\
te\\ J 4.3 B-Pan+Larvzewelght
\\
(mg) 41?
e e
Larvae welght (mg) - A - B ACi
_(&
I V
Weight per Initial number ort
%\\A~1.
~
IN A
larvae (mg)
Ax 0,\\
l 5
, t"o
- C I Inillal number of larvae O
O 0
')
O*
O Average weight per Initial number oflarvae (mg)
Calculations and data reviewed:
Comments:
I I
1 I.
4 I
1 1
I I!
I I
..7-f6-
- nvironmental Testing Solutions, Inc.
Page 4 o
- nvironmental Testing Solutions, Inc.
Pane 4o f6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
65-o6-o0l Survival and Growth Data Day 100% Intake Y
Z I AA BB O~/
.IC l) l l).
2
/0 10 I
C /b 2
/0 10O
/0 /0 3
Jji7 6
5 4
(
q q
/0 6
/
A - Pan weight (mg)
^ \\Ps '
\\& -fJ4 ?W >
B - Pan + Larvae welght
\\
(mg) r, Larvae weight (mg) - A - B A:
Weight per Initlal number of larvae (mg)
- C I Initial number ofarvae Q
- CO P
D Average weight per Initial number or larvae (mg)
O. C)O5 Calculations and data reviewed: 4_
Comments:
Ir be
&&laJ A
kojQ V it
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R.02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification ofData Entry, Calculations, and Statistical Analyses Clietc TVA/Seqwuyabl Nlleakr Plant -.26.4rtated Tait dates:
Msy 6-13. 2004 Projet number.
1206 IlevIweM yy~
c*_.-~ (%
w
.... f
-1
^_
r__
t~..I
- . t
- 1-2*W L{f L.......................~
M.3 W.IMS..i.4
~
U
.I~.*.r
~k.
d..f.I*..
.wl
- t__b",
C~b,,^k*.t.V *1.- \\
C_ P... t.".^
.. Itkt(md
-A-l
-a.(..)
-(w3 h..
(.)
(fC) 1 rib.2
(%)
A I 0 IU3 14.41 2J.15 S.67 0.67 0.167 Cotro1 C
10 t0 14.51 23.73 9.5 0.915 03.69 3.t 0915 100.0 O.61 3.3 Not applicabte C
t0 I20 14.69 21.06 1.37 0.137 0.137 D
10 20 14.41 22.99
- .55 O
0.t5 E
10 10 14.96 23.21 3.25 o0s25 0.325 209%
2 I20 2 0 14.17 21.15 3.91 0.391 1711O8810.
.6 1
J 0 C
0 15.25 24.13 9.58 0.958 0.t67
- t.
0591 100.0 0.367 3.3 0.
It 20 20 14.67 22.53 7.s6 0.786 0.786 I t 1I 0
14.96 24.46 9.50 0.950 0950 22A 10 I0 14.41 22172 3.31 0.813 0.1112
- 22.
0132 100.0 0.332 11.3 4.3 K
20 I 0 24.91 22.217 7.24 0.724 0.724 L
t 0
25.21 23.39 1.21 0.J21 0.t21 20 I10 15.03 22.67 7.64 0.764 0.764 41.9%
O 10 10 15.10 23.30 19 0319 42O05 1u.0 0.S29 100.0 O.tO5 13.0 7.4 o
io 2 0 25.20 24.52 9.42 0.942 0.942 P
20 0
15.02 21.96 6.94 0.694 0.694
-Q 20 10 14.9S 24.13 9.20 0.920 0.920 7
R I20 20 14.30 23.73 1.93 0.393 08271-.9-10002271-.
S 20 20 14.77 24 27 9.50 0.950 Qt92 7.1 0393 100.0 O.t92 7.2 t2.6 T
20 10 14.70 22 74 S.04 0.504 e 004 U
0 20 24.37 23.09 1.22 0122 0522 100%
v t0 t0 2462 23.37 3.15 0.737 0.395
.50.137 100.0 0.395 6.5
.3.0 W
to 1o 14.62 23.97 9.36 0.936 0.936 X
0 0
14.33 23.10 9.47 0.947 0.947 Y
2O 10 14.91 24.64 9.73 0.971 0.973
%ate Z
10 9
24.9 22.62 7 7.70 2-02.
0.952 7.4 0.770 ss.
0.ses I0.2 4.
8AA 0
9 14.92 24.14 9.22
.0294 0.922 2322 I20 290 24.57242 9.54 0.954 0.954 J__
I Owifall 202 DoAnnel's ISD value:
PMSD:
lottle-Dmnmet
!S MSD vatlv:
PMSD:
0.1280 14.1 0.0902 23.0 MSD-iMSnSirannt DilTrcene PMSD -
Paenrct Minimm Siiitknt Differ nee PM SD) i2* anu.w of les prae2iou.
The ?M S?) i.22 t ampnr tc t difreanou betw~~t li control tcnd trebn at thS ca bt t declared atzt*ialyc l i rL t It I Whole effluent loart y let.e On awe o a as i rcant diffen~
tOecuf fe fiolnaiou~ n tatl FtetiniSoluticoa. Inc. durc2*t~ xctm y lath whem a toxicant hesdu t IR meA aes z wth by 2515 nt Sa acntrol Cdactaind a1+/-olt refermnc, toxicant lest2i*
LtwerPMSDboutnddtenrTinedbyUSE!PA(010h percent)
- 9.4K U
er PMSD bound detwrined by USEPA (90th prntie) - 35 Sb low and upertbound we. eeutctd bytho USEPAxsing205 txtS oonudcted Sm 19 lotoortortPf 1ale, Voth in ciroioc rcrnc toxiat tets.
USEPA. 2000. Uadentmdiand ounthg Method Vibiliy iWhole Efflent 7ositAppliatino UndertheNatinal lolhatant DirgeE ntio Pwun BPAtl- -00.tJS Envlr talPretion abets Chneinnit OIL
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/6/2004 Test ID:
PpFRCR Sample ID:
TVA I Sequoyah Nuclear Plant - Outfsll 101 End Date:
5/13/2004 Iab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
PP-Pinephales promelas Comnmnnts:
NON-TREATED Conc-%
1 2
3 4
D-Control 0.8670 0.9150 0.8370 0.8580 10.98 0.8250 0.8980 0.9580 0.7860 22 0.9500 0.8310 0.7240 0.8210 43.9 0.7640 0.8190 0.9420 0.6940 72 0.9200 0.8930 0.9500 0.8040 100 0.8220 0.8750 0.9360 0.9470 Transform: Untransforned Conc-%
Mean N-Mean Mean Mln Max CV%
N D-Control 0.8693 1.0000 0.8693 0.8370 0.9150 3.795 4
10.98 0.8668 0.9971 0.8668 0.7860 0.9580 8.829 4
22 0.8315 0.9566 0.8315 0.7240 0.9500 11.133 4
43.9 0.8048 0.9258 0.8048 0.6940 0.9420 13.026 4
72 0.8918 1.0259 0.8918 0.8040 0.9500 7.061 4
100 0.8950 1.0296 0.8950 0.8220 0.9470 6.488 4
1-Tj t-Stat CrHI 0.047 0.711 1.215
-0.424
-0.485 tiled Isotonic tical MSD Mean N-Mean 0.8693 1.0000 2.410 0.1280 0.8668 0.9971 2.410 0.1280 0.85S8 0.9845 2.410 0.1280 0.8558 0.9845 2.410 0.1280 0.8558 0.9845 2.410.
0.1280 0.8558 0.9845 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-WilkW Test indicates normal distribution (p > 0.01) 0.97593939 0.884 0.19275556 -0.3327248 Bartlett's Test indicates equal veriances (p = 0.59) 3.6894629 15.0862722 Hypothesis Test (1-tall, 0.05)
NOEC LOEC ChV TU MSDu IMISDp AISB MSE F-Prob df Dunnett'sTest 100
>100 1
0.12797607 0.14722585 0.00498307 0.00563967 0.51204991 5, 18 Treatnents vs D-Control sqn._OS0-06-04dtaf
I..
Environmental Testing Solutions, Inc.
Statistical Analyses wine2rTetirolhtlon (200 ResanVes)
Skew Point SD 95% CL(F~rp)
IC05 ICIO iC'5 IC20 1C25 IC40 IC50
>100
>100
>100
>100
>100
>100
- >100 1.O, 0.9 0.8-0.76 0f 0.4 r, 0.3 08 0
c- 053' 0.2-a.t 0.0.
-0.1 0
20 40 60 so 100 Dose %
120 Dose-Rspowme rlot I. U 0.8 0
a 0.82 0a -
- 0.4 0.2 n.
I--
--I 1-tail, 0.05 flar of signIftance 0d 9a is 0,
ra sqnO5-06-Odama
Environmental Testing Solutions, Inc.
Statistical Analyses I
Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/6/2004 Test ID:
PpFRCR Sample ID:
TVA I Sequoyah Nuclear Plant - Intake End Date:
5/13/2004 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Protocol:
CIIRONIC.EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Cornments NON-TREATED Conc-%
1 2
3 4
D-Control 0.8670 0.9150 0.8370 0.8580 100 0.9730 0.7700 0.9220 0.9540 Transform: Untransformed I-Tailed Isotonic Conc-%
AMean N-MAean Mean 5i n Afar CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.8693 1.0000 0.8693 0.8370 0.9150 3.795 4
0.8870 1.0000 100 0.9048 1.040R 0.9048 0.7700 0.9730 10.198 4
-0.725 1.943 0.0952 0.8870 1.0000 Auxill2ry Tests Statistic Critical Skew Kurt Shapiro-Wilkl's Test indicates normal distribution (p > 0.01) 0.88513231 0.749
-1.4024664 2.44750925 F-Test indicates equal variances (p - 0.13) 7.82257462 47.4672279 Hypothesis Test (1-tail, 0.05) hfSDu MSDp MSB MISE F-Prob dr Homrscedastic t Test indicates no significant differences 0.09520179 0.10952176 0.0025205 0.00480058 0.4959721 1,6 Treatments vs D-Control sqnOS-06-04data
Environmental Testing Solutions, Inc.
Statistical Analyses Linear Inrdepobatln (200 Resaniples)
Sktew Point Icos ICRC 3C15 XC20 L'25 1C40 1C50
>100
>100
>100
>100
>100
>100
>100 1.U0 0.9-QB.
0.7 -
0.6 -
e 6 c 0.5-0 C 0.3 0.2 0.1-0.0
.0.1 1 I...,....,.-.....
0 20 40 60 so 100 Dose %
120 Mse-Reponi Piot 1.2 1g-08 o 0.6-a
'- 0.4-0.2-1-tafl. 0.05 level Iof slgnlllcance fl-4 9
snO05-06-04datna
-%-,v
-~'>
E.
Tetn S
i n
E£nvii-omarenfal Testing Solutions, Inc.
w sas;^<rI Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephialespromelas Daily Chemical Analyses Client:
TVA Test dates:
May Project number:
1206 I Sequoyah Nuclear Plant, Non-treated 06 -13, 2004 Reviewed by: (
)/
Cuncent rution Parameter Day 0 Day I
)ay 2 lDav3 Day 4 1)2 S Da Initial Initial IFnal Initial Final Initial Final Initial l
Initial Final Initial Fial pi1I (SU) 7.68 7.55 8.02 7.54 8.05 7.53 7.X2 7.44 7.63 7.38 7.64 7.25 7.49 7.47 DO (mg/L) 7.9 S.0 8.0 7.5 7.9 8.1 8.3 7.8 8.2 7.7 8.2 7.3 8.0 8.2 Control Conductivtty(jfmhosfcm) 300 218 307 302 CAlkalinity (mg/L CaCO2) 64 60 62 Ifardness (mg/L CaCOI) 82 82 82 Tempnerture (CC) 25.3 24.2 25.0 24.4 25.2 24.3 25.2 24.1 24.8 24.2 25.0 24.2 252 24.1 p11I1SU))
7.76 7.S2-7.70 7.51 7.68 7.53 7.69 7.37 7.58 7.40 7.56 7.26 7.5247 10.9S%
DO (mg/L) 7.9 8.0 8.1 7.5 S.1 8.1 8.3 7.8 8.2 7.7 8.2 7.3 8.0 8.2 Conductivity (Itmhostem) 299 300 276 288
_i27__273 294 1
Temperature (C) 25.2 25.1 24.5 25.0 24.3 25.2 24.1 24.9 243 24.9 24.2 24.9 24.1 p1t (SU) 7.74 7.51 7.68 7.51 7.65 7.52 7.68 7.37 7.55 7.38 7.54 7.26 7.48 7.47 22%
DO (mg/L) 8.0 8.1 8.2 7.4 8.2 8.1 8.2 7.8 8.2 7.8.
8.2 7.3 8.0 8.0 Conductivity (trnhos/em) 288 287 270 273 264
____3 28 Temnperture (CC) 25.2 24.1 25.1 24.3 25.0 24.3 25.2 24.0 24.9 24.3 25.1 24.2 25.1 24.1 1i (Stl) 7.72 7.44 7.67 7.62 748 7.64 7.40 7.49 7.41 750 726 7.44 7.48 9%
)O (mg/L) 7.9 78 8.2 7.4 8.2
.2 82 7.8 8.3 7
8.2 73 8.0 7.9 Conductivity (Ornhos/crm) 262 26 248 252 249 240 239 Temperature CC) 25.4 2411 25.1 24.4 24.8 24.3 25.2 243 25.1 24.3 25.2 24.2 25.1 243 DilI (SU))
7.711 7.431 7.641 7.491 7.621 7.52f 7.601 7.36 7.431 7.391 -
7.471 7.281 7.381 7.48 72%
Conductivity (Itmho*/cm)
-I 221 I 207 209w.
21_
DOemnegtreL) 8s41 2.0l 251&27.S 24.61 8.1 2.21 74.9
.1.
.]
.11 8
Tenvtr l
25.41 24.11 25.11
-24.41 24.61 24.31 25.21 24.1 25.31 24.21 24.2 24.1 7.47 IX) (mgfL) w- -
And t
loo%
nilI (SU)
Conductivity bmbn5/cm)
DO (ma/U)
Conductivity ((urnfho/)
I Alkslinity (mg/L CsCO,)
E I DO%
Tmardnar(mrLC1SCOC )
Total Rlesidual Chlorine (mg/L)
Temperature CC)
I-
~
~
7.691 7.481 7.621 7.51 7.61 7.561 7.571 7.41 7.36 7.44 7.43 7.
7.9 7.8 8.1 8.3 8.1 8.3 7.9 8.3 7.9 8.2 25.4 24.1 25.2 24.3 24.5 24.3 25.2 24.1 25.3 24.2 25.4 24 7.68 7.57 7.61 7.57 7.61 7.47 7.61 7.37 7.71 7.53 7.62 7.
8.0 8.0 8.3 7.5 8.3 8.0 8.4 7.8 8.4 8.1 8.3 8.183 19 191 183
-18318-7_
60 66 6 75_
73 7 QUl _<.10 02_
25.: 1 24.21 75.1
- 2.
24.8 24.3 25.2 42 2
5.1 1 243
- 2.
24 27 nIl (SU) i~
DO (mna/U Alkn11nity (mguL CaCO,)
100% Intake Conductivity (trhas/ci) l Alkalinity (mrh/L. CaCO,)
ixardness (mcg/L CCO1) f -
Total Residual Chlorine (mg/L.
l i
\\
-w l
Trmner2fure IM
Environmental Testing Solutions, Inc.
Page 5 of 6 Eniomna Tetn ouin, n.Pgso Species: Pimeph alespromelas Client: TVA t Sequoyah Nuclear Plant - Non-treated Date:
CS lo-oq 1
I' t
U I
I I
I
.1e SI 4*4 CI I
Daily Chemistry:
Dav
.0 I
2 Analvst Concentration Parameter CONTROL pH (S.U.)
. b&a
.5 q
v n.
DO (mg/L)
I.q 9
Conductivity (pmhos/cmn)
Alkalinity (mg CaCO3IL)
(D'A Hardness (mg CaCO 1/L) 82-Temperature( 0C)
(
7i-24.2-Zs.O 2
o..L 2SZ ZY3 pH (S.U.)
Le2..O
\\S3 10.98%
DO (mgeL) 0 Conductivity (jim hos/cm )
Temperature(eC)
(Q 2S. Z Z7-Zs.l 7--bS
'2S.O Zq.1 pi (S.U.) 14 4.51
___ od I-ISt DS
.c rs 22%
DO(mg/L)
__.___O I
1.A A
Conductivity zI (pmhos/cm) fSS Z_87 Z_
I Temperature (C) 25.2 2.1I.s.*
2 v4-3
_25. a p11 (S.U.)
':.L44 i
t03
-4; Z.@
43.9%
DO (mg/L) re Conductivity 2
(pimhos/cm)
_ET 2.__
Temperature (°C) 25.-l 2q. II ZS pll (S.U.)
I z
-3
- q.LI
-l1 q A.A 2
5 7
72%
DO (mglL)
.0 6
'1.S k.- 2.1 Conductivity 2-(pmhos/cm)
- 2 ____
Z_
__Zl Temperature (°C) jZS Y
ZLI.
Z75*.1 \\
W.(o Z
p~l (S.U.)
_N1 ls
--I5 -. (D2 I-Sv
.X 5
100%
DO (mg/L)
- t. B.1 1.6 g.3 Conductivity
.q3 (Vmhos/cm)
Alkalinity (mg CaCOAI)
Hardness (mg CaCOL TR chlorine
- 0.
)
0
< 0.10 (Mgf)L)
Temperature (C) 25.s Z'.r.
zs..
z7 t4.7 Z-2 pii(S.U.)
i 7.(v 2-51
- lol 1.5 i
i.(t o
tI1 100% Intake DO (mg/L)
.o 1.D 3
Conductivity (limhos/cm)leI Alkalinity D\\o (mg CaCO3 1.L)
Hardness 13 (mg CaCOAI)____
TR chlorine7 (m cfL)
< O._____
(
Temperature(°C)
- 25.
- 2.
ZS -i Z2. Z Initial Final Init'al Final Initial Final
Environmental Testing. Solutions, Inc.
Page 6 of 6 Environmental Testing Solutions. Inc.
Page6oF6 iiI...-I,,
I-I I
- I i1 I
I I
I 4
ii.;
I I
I I
I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
OS -oD-6
-~
ly 3
4 5
6.
Analyst C-M Isr Concen-Parameter tration No CONTROL pH (S.U.)
1AL?2
-J1 T1.e 3 7.S % &
S
.4 9
DO (mgeL) 6.3
- 1. _
87>
5R.2
-. s 0
Conductivity
(. mhos/cm) 2.61 Alkalinity.
(mg CaCO)
\\).
Hardness (mg CaCO31L)
TemperatureC) i5 2.2 2t4. I v4.8 24Z IS
.C.
'A.7-IS.2 4.1 pH (S.U.)
.1 35B
'7AO P
1*.2 LO 1.S5727 10.98%
DO (mg/L) 1.6 8Z.
1q In 1.R 3.c 3.2 Conductivity Z273
_2__
(umbos/cm)
_____2____
Temperature(°C) 25.2.
jZ A
F-Z'A.;
zT I 2 A
?,AA pll (S.U.)
-4. 0 a 1-31 7.5S5 17-5
-3
-7.2,
-ZW 1.
n-W 22%
DO(mg/L)
- 0.
7.Z 1.b 1nX fl Conductivity 2z I Za2
\\
(pmhos/cm)
_______ME Tcmperature (C) 25.2 IA z.O 5*.
2S.l 12
'zS.-I l
4A p11 S.U.)
X
.I&
.9 1-7.q
- 7. 4 1
-1.o
'7. -Z L
-IL44 LIP)e
- 43.9%
XDO (mg/L)
=
9Z n-f w
32 7-S St Conductivity 77 (gmhos/cm)
Z5 Z
_2________
Temperature (T)
Z5. 2
'Z43
%5_. 1 7-!-_2S
.Z
__S_.Z 2.S.
pH (S.U.)
-+3(.00
- 1. 3 (0 17 43 139
'7.!J'
-7. V
-n.'IE S.e>
72%
DO (mg[L) 7 3
l
- 1.
5
.8 Conductivity 2Zzl w
1 (pmhos/cm) 2ZI
- woo, Temperature (°C)
.. 2cz 2q4.*
7S.3 a
'-Z
.s Z Z.
-2.Z.
zs&
,A 4 pit (S.U.)
-1.S3 1
l 13D.
1.- +3
'1-Z
.7 35
-V. q 100%
DO (mz/L)
.3..
9 17.q A.4 6.
4 Conductivity I61o (pmhoslcm) 173 Alkalinity
.(me CaCOIL) \\01 Hardness TR Chlorine (mgtL)
_0._I 0D_
Temperature (0C)
Z5.
Z.
1 2S. 3
-L-.7..S. S 2.Li*7 2L.
2'bL pEI (S.U-)
l 73 ni
.n1 l7 *-t?
'I iJL
-:t to b
- 7. (e 7 l5 100%
DO (mg/L) 4 Th7 8,Z Intake 6
Conductivity lei (pmhos/cm)
Alkalinity (mg CaCOAI)
Hardness (mg CaCO,/L)
Temperature (C)
I1.2. 'L
. I 759.
2I I5 2, Z
. ?_
ZI.2..
Ini ial Final Initial Final Initial Final Initial Flnal
Environmental Testing Solutions, Inc.
Pagc l of 7 Enviromenta Tetn ouinIc ae1o Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah Nuclear Plant-Non-treated NPDES fi: TN 0026450 Project #:
tzo(O Dilution prepara ton information:
Comments:
Dilution prep (%)
10.98 22 43.9 72 100 Effluent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 700 0
Total volume (mL) 2500 2500 2500 2500 2500 Test organism information:
Test information:
Organism age:
4C A.
ZS 45 C.
Randomizing template:
E bL Date and times organisms Os-ek-e a s53m o S4i Incubator number and were born between:
shelf location:
ZE 3 Organism source:
Otl-z.-oLl A-C I YCT batch:
AR8 042i*O4 Transfer bowl information:
pH = 7.11 Temperature = 2.9 I Selenastrum batch: A5$ oq ZloLI Daily renewal information:
Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time 0
OSOL-Q'4 I1z3 a
OS -cA x-.z3 q
A o
os ds.o I
C6_
1
-sosa.O I.- 45 A-2 So a-csnc-t oeD)-
A -
3 C;Mtl l >)
OS-M-aj OOY)bt?.(yi I e
4 o-o'4 l l bOiO5-.QI l.0 2Z s
5 5-11l-b 1_ _OS O
-O 4 oqoq.b1 (
.6 I.
6 OS l2 g0I II OA 0511Gt tt)!Zq. c
.O s A
05 O{
I Ob H
l 1
Control information:
trL0LC-42 Acccptance criteria Summary of lest endpoints:
% of Male Adults:
s 20%
7-day LC50
> l Ot\\
% Adults having 3rd Broods:
lOw.
l 80%
NOEC rt,
% Mortality:
07 S20%
LOEC j
6`7' Mean OffspringfFemale:
.* 3l. _
2 15.0 offspringremale ChV I
6!9
%CV:
_7. IL7
<40.0%
IC25
> i W7.
CmI4L Co~M l.
Environmental Testing Solutions. Inc.
Page 2 of 7 i"
i
.'1I Species: Ceriodaphnia dubia Client: Seniuovah Nuclear Plant - Non-treated Date:
cS-ckScfA CONTROL-I Survival and Reproduction Data Replicate number Day 112 3
4 5
6 7
8 9
10 I
Young produced O
I O
0 0
0 l
)
0 Adult mortnllty l
I L
t L
+-
2 Young produced C
)
C
)
C Adult mortality T
I 3
Young produced C)
C)
C) g
- C Adult mortality L
t X%.
%,.- IL__
4 Young produced 3-4 Adult mortality
\\
L J
t l
U S
Young produced Q 1 I*
Ijj zJ Q
I --
ll I C) 10 Adult mortality l
.U Ll Ll Lt ll L
6 Young produced j0 0
- 10.
(')
I Adult mortality l
L..
L-I l
L 7
Youngproduced 1
13 l1 I to l-I to I -I iLi IlL4
-I1 Totalyoungproduced 21 33 37z l E l3.l 31 l z
l
?
2P 3 2 Final Adult Mortalltv l
L l- -
t2 L l -.
=
l
-W*
Xfor 3" Broods l
>cl
)C I
I l
Concentration:
I
% Mortality:
l O7 l
Mean OffspringlFemale:
3o..
l CONC: 10.98%
Survival and Reproduction Data I
lReplicate number Day 11 2
3 4
l 5
16 7
8 9
10 lI lYoung produced l)
@l(1<
l(l2 l°l°1C Adult mortality llLl L
U...t l
2 Young produced o
j
~0 0
1 Adult mortality It 1 L
(
L I U L
L Ul L
1 L__
3 Young produced O
0 0
0 f
o r
1=
tiL 5
1
° Adult mortality t
1 U.
l L-L L-L ji I
i 4
Young produced LilI' 57 co l
Adult mortality L
L U
I.L LI LI ll L
U L-s_
lk j
i I
Y ung produced
-Li-
-1 Adult mortality L
L-L-l r
c::-
L 0
0 0
l6 lYoung produced 1
A dult slortality T
U.
U U
UL T
7 Youngproduced l.
11 15 l
Is S
l 13l Total young produced I
I 3
52 1
32,.
3 Final Adult M ortality l
L 1
W L...
l Concentration:
% Mortality:
i I Mean Offspring/Female:
I32.
1 I l Reduction from Control: I - 1.37o l
I.
Environmental Testing Solutions, Inc.
Page 3 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: 052,-o' CONC: 22%
Survival and Reproduction Data Replicate number Day 1
2 1 3 4
5 6
7 S
9 10 1
Young produced J) rI o
O l
0 0
o C
Adult mortality i-r U
2 Young produced l
0 0
0 C
Adult mortality Y
U.
L L
L U
L 3.*
Young produced C
O O
Adult mortality U
U U
4 Young produced a
(=
'I S
Adult mortality UK.
U
\\
U U
W s
Young produced L
t OZI Ol Adult mortality
\\._
-U l
U U
L-6 Young produced O
o l
(D ltA
- IAdult mortality' L
U L-U U
U U
7 Young produced li-i LA~(i-1 t~'-
I '5 I'S I
Totalyoung produced 3(o 2e1 3b a51 33 3
37 38a Final Adult Nortality w:
l Concentration:
%Mortality:
I 1 I Mean Offspring/Female:
33-.
I
% Reduction from Control: lcUl9?7.
CONC: 43.9%
Survival and Reproduction Data Replicate number D2v l
Il 2
3 4
5 6
7 8
9 10 I
Young produced l n
l F Adult mortality l_
=
U U
Ll 2
Young produced lO 0
)
Adult mortality J L:
~
L U
t
~
UU 3
Young produced
.Adultmortality T
L L-LI L
U L
L L
L l
l Adult mortality L
Young produced Adult mortality l
l ll Total young produced a'6 Oq 34aq 35 q
3-1
.3Z2-FInal Adult MlortAl~ty U- *-1 T IL L...
L....
1...
1...
I %L.
1t* SPor I64061)
Concentration:
% Mortality:
I 7.
Mean OffspringFemale:
I EReductionfromControl:
P.2%-
Environmental Testing Solutions. Inc.
Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date: c;-o~cA CONC: 72%
Survival and Reproduction Data Replicate number Day 1
l 2
3 4
5 6
7 1
8 9
10 I
Young produced
-Q -
_C O
O Adult motlt Adulmtl L.-
q l-2 Young produced l
(
C>.
C)
I £ CC Adult mortality l
t l
L
-l
\\
i 3
Young produced l
j C
.c.
C.
..c...
0 Adult mortality
\\-L -
L.
LU U
4 Young produced
- 5.
5 5
S_..
S Adult mortality L-L-
s Young produced 5
- 5T I4
.5I6I(US I Adult mortality L
L x-.-
6 Young produced IO O
C) CL IZ l I iS 12.
Adult mortality l
L.-
l 1
u 7
Young produced 4
1 lf I
I
-O I1 7otalyoung produced li 55 3
l 3b 3 (D T T 3a.
59 I T3Z Final Adult Mortality
- t.
17 L.
=.i i
t i ii Concentration:
% Mortality:
0.
I Mean Offspring/Female:
1S. 1
% Reduction from Control: -16..7.o l
CONC: 100%
Survival and Reproduction Data I_____
Replicate number D2v l
1 2
3 4
5 6
7 8
9 I
10 I
Young produced lQ l
(
l O
Adult mortality L
-L L
I-Adult mortality lLl l*
L-L-
L-L-
1-l 3
Young produced l
O l lll Adult mortalty L
L Young produced I s2 p
- 5 16 IS (O
Adult mortality
_U l
Young produced il 15 r
1 3
(_
5 Adult mortality 1
- r L
L.'
L L
6 Youngproduced I
Io T
Adult mortality U-TJ-JL LfL.-U 7
Young produced 7
-ao a
a.1 a
Total young produced 4
5%
6 3~
237 3G 39 -8 a 3 w
8.S Final Adult Mortality T
l Ul.l L
l 1
l l Concentration:
%Mortality:
0l.
l Mean Offspring/Female:
l 3c.4
% Reduction from Control:
-20.57.
Ui,"
ctr-.
C" rvc,--
1 'I I1
Environmental Testing Solutions, Inc.
Page 5 of 7 Species: Ceriodaphnia dubia Client: Senuovah Nuclear Plant - Non-treated Date:
OS-C65ot-A CONTROL -2Z Survival and Reproduction Data Replicate number Day I 1 2
3 4
5 6
7 8
9 la
.1 Youngproduced i
.C 0
C(
O 0
C)
Adult mortality L
N--.
L-U I.-
2 Young produced l
+
l ().
C.
0
- c.
lL O O
Adult mortality l
t.
3 Young produced j2 3
O Q _
~Adult mortalltyl _.. t _.
4 Young produced S
L Adult mortality JL L.
'U_
L.
U U
5 Young produced 1L7:
- 2
-tI0
-2 Adult mortality U.
L L-L-.
I L-1-~
L-6 Young produced iT Il Adult mortality 7
Young produced C)
Is Ij )
OO O
lOl
)
4
~0 7oa young produced l
- 5 l1 5l13 l
8llb 1
Totalyoungproduced j
53
- 32.
2 "ij 2S 35 31
.3 22 3(o Final Adult Mortality l
l t l
L..I l l
l Concentration:
% Mortality:
7 l Mean Offspring/Female:
l 31.
I
% Reduction from Control:
I.P CONC: 100% Intake Survival and Reproduction Data
[
I__
Replicate number l
Da l
2 3
4 6
7 8
9 1
0 l
I l
Young produced l
l Adult mortality L (
L -
l L
2 Young produced
~O...Q C
c c
- 1.
i~c Adult mortality lLI L
LLl I
lLI l
T l
3 Young produced o
° 0 LQ l
O. I Adult mortality l
L lL l
L l
ll L
lL lL 4
Young produced S
4 5~
I Adult mortalIty l
U_
\\.
l-l-
4 l
5 Young produced AQ.
12.. 1 0 1,
I O QC Adult mortality l
T l
l lll L-U U
6 Young produced o
I.L 2.i-QC
'.)1.
j Adult.mortality l
U.-
l l-1-
L lll 7
Young produced I~ J5 IL J 78 Is,~JW1 Total young produced 12-T j
'5l
-j aS 3
B3LI B(
3 q 2c1 a2-Final Adult Mortality l
1I I
I I
IW C
I C
Concentration:
% Mortality:
07.
Mean OffspringpFemale:
31A l
% Reduction from Control:
OfstNI 1
I 3-iI i..
I.
I
'I 1,.
Environmental Testing Solutions, Inc.
Verirication or Ceriodaphnla Reproduction Totals Control-i Re Plcate number Day I
l 2 l 3
l 4
5 6 l 7
8 9 9 1
Toba1 o
o
- o.
- o.
o o -o-
- o.
o I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
4 4
5 3
4 4
5 4
5 42 5
0 12 I
1 12 10 13 10 10 89 6
10 10 0
0 0
0 0
0 0
0 20 7
13 13 17 16 14 16 17 141417 151
'Total 27 27 33 32 28 32 31 32 28 32 302 10.98%
Day Re Iklete number Total 1
6 9
10-1 O
O O
O 0
O O
O O
0 2
0 0
O O
O O
O O
O O
0 3
'O O
O O
O O
O O
O 0
4 5
4 5
5 3
4 5
5 6
5 47 5
.11 14 11 13 14 12 0
12 13 12 112 6
0 0
0 0
0 0
1 0
0 12 7
16 14 14 17 15 I5 14 13 17 13 150 Total 332 2
30 35 32 31 31 32 36 30 321 22%
Day Re licate number Total 1 2 3
4 S
6 17 8 S 1
0 0
O 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 6
4 6
5 5
5 5
6 5
52 5
14 12 11 13 14 14 12 12 14 11 127 6
0 0
0 0
0 0
0 10 14 7
17131417 16 14151518 0S 3139 Total --
36 31 9-6 T35-33 32 32
_38 30 332
h 43.9%
Da ~
2 Re Alcetv number Toa Dy 1 2
314 5
6 17 8
9 10I oa I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 O
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
5 5
5 4
5 5
6 5
49 5
14 13 13 IS 12 12 12 14 13 10 128 2 l16 16 19 26 17 19 13 18 0
136
-2 I
35 3
72%
lDy Replicate number T tII D Y I1 2 l 3 4
S 6 l 7
-8 9
To10 t
I 0
I o I o I ol o T o I 0
21 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 5
5 4
5 5
6 5
4 5
5 49 531113 21 12 13 14 14 1 1 15 12 131 6
16 0
0 0
18 00 1
15 50 7
lI 1
151 18 19I 0 16 17 17 18 0
121 Total 37 33 34 36 36 36 36 32 39 32 351 100%
Day Re licate number Total I
3 4
5 6 l7 8l 9
10 I
0 0
0 0
0 0
O 0 o o
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 6
5 4
5 5
6 5
6 S
52 5
12 13 15 14 14 14 15 12 14 141 137 1
6 1 1 0
0 19I 0 01 0 0
0 1 16 36 7
1 18 17 15 0
17 20
-18 16 18 0
139 Total 36 36 35 37 36 39 39 33 38 35 364 Control-2 Da Replcate number Total Day 1
2 3
4 5
6 8
9 10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
5 6
4 5
5 4
6 4
6 49 5
12 12 11 10 10 13 12 12 1 1 14 117 6
14 0
0 0
0 0
0 0
0 1
15 7
0 1
15 is 15 13 17 15 18 13 15 136 Total 30 32l 32 l29 28 35 31l 36_
2 36 317 100% Intake l
Da lR Ileate number Total D
1 2
3 4
S 6
7 I 9
Tt 1
0 0
0 0
0 0I 0
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
4 4
6 4
4 5
5 4
4 44 5
10 12 0
0 13 10 0
0 0
13l 58 6
0 0
12 11 0
0 14 12 12 0
61 14 15 14 14 18 16 115 17 13 151 151 Total 28 31 30 31 35 30 34 34 29l 32 314
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA I Sequoyah Nuclear Plant - Non-tteated Client:
Test dates:
M1ay 06-13, 20W4 ProJect number.
1206 lRvelywed by: A/) I A4-Concentration
__________RelcteI~
number
_____Survival Average reproduction coemcelet at Pentat raduction from
)
1 2
3 4
5 6
7 8
9 10
(%/)
(offtprnn/rtmale) variatom(
pooedcontrote (%)
Control-I 27 27 33 32 28 32 31 32 28 32 100 30.2 7.9 Not applicable 10.98%
32 32 30 35 32 31 31 32 36 30 100 32.1 6.1
-63 22%
36 3 1 29 36 35 33 32 32 3S 30 90 33.2 828
-9.9 43*9%
35 34 34 39 33 33 36 32 37 32 100 34.5 6.6
-14.2 72%
37 33 34 36 36 36 36 32 39 32 100 35.1 6.5
.16.2 100%
36 36 35 37 36 39 39 33 38 35 100 36.4 5.2
-20.5 Control - 2 30 32 32 29 28 35 31 36 28 36 100 31.7 9.8 Not applicable 100% Intake 28 31 30 31 35 30 34 34 29 32 100 31A 7.4 0.9 Outrfij 101:
Dunnett's MSD value:
PMtSD:
Intake:
Dunnett's MSD value:
PMSD:
2.369 7.8 2.120 6.7 MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole emuent toxicity test On avenge, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.2% from the control.
Lower PMSD bound determined by USEPA (10 lpercentile) " 11%.
Upper PMSD bound determined by USEPA (90d percentile) 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 labortories for Cerfodaplnua reproduction in chronic.
reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole EMuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA.833-R-00-003. US Environmental Protection Agency, Cincinnati, 011.
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphnla Survival and Reproduction Test-Reproduction Start Date:
5/612004 Test ID:
CdFRCR Sarple ID:
TVA I Sequoyah Nuclear Plant - Outfall 101 End Date:
5/13/2004 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Pmtocol:
CHRONIC-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comrnnts: NON-TREATED Conc-%
1 2
3 4
5 6
7 8
9 10 D-Control 27.000 27.000 33.000 32.000 28.000 32.000 31.000 32.000 28.000 32.000 10.98 32.000 32.000 30.000 35.000 32.000 31.000 31.000 32.000 36.000 30.000 22 36.000 31.000 29.000 36.000 35.000 33.000 32.000 32.000 38.000 30.000 43.9 35.000 34.000 34.000 39.000 33.000 33.000 36.000 32.000 37.000 32.000 72 37.000 33.000 34.000 36.000 36.000 36.000 36.000 32.000 39.000 32.000 100 36.000 36.000 35.000 37.000 36.000 39.000 39.000 33.000 38.000 35.000 Transform: Untransformed I-Tailed Isotonic Cone-%
Mean N-Mfean Mean Alin Msx CV%
N t-Stat Critical MSD Mean N-Mean D-Control 30.200 1.0000 30.200 27.000 33.000 7.929 10 33.583 1.0000 10.98 32.100 1.0629 32.100 30.000 36.000 6.135 10
-1.834 2.287 2.369 33.583 1.0000 22 33.200 1.0993 33.200 29.000 38.000 8.844 10
-2.895 2.287 2.369 33.583 1.0000 43.9 34.500 1.1424 34.500 32.000 39.000 6.588 10 4.150 2.287 2.369 33.583 1.0000 72 35.100 1.1623 35.100 32.000 39.000 6.504 10
-4.729 2.287 2.369 33.583 1.0000 100 36.400 1.2053 36.400 33.000 39.000 5.213 10
-5.983 2.287 2.369 33.583 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates nornal distribution (p > 0.01) 0,70720047 1.035 0.2032235
-0.7907342 Bartlett's Test indicates equal variances (p - 0.82) 2.18661237 15.0862722 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100
>100 1
2.36943653 0.07845816 49.7366667 5.36851852 2.113-06 5,54 Tren.jtn-tq vs T.Contrmt sqnO5-06.O4data
Environmental Testing Solutions, Inc.
Statistical Analyses LlnearTnterpol2tlOn (100 RtftrieS)
Sbew rota
/.
ST) 95% CL 1C0s Kda IC15 1C20
- IC2S 1C40 1cs0
>100
>100
>100
>100
>100
>100
>100 1.0 0.9-0.8 0.7-0.6-a 0.5-
° 0.4 a
i* 0.3 -
0a 0.2-0.1 2 0.0 0.1 0.2 0
20 40 60 80 100 120 Dos* %
Dose-Rsponse Plot AC 40 35.
C 30 U 25 2 20 CrIs.
10.
5.-
..1.
1-tah. o.Os le of sIgnlicance 0+
090
-0 0o V!
(-)
N spn_05-06-04data
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphia Survival and Reproduction Test-Reproduction Start Date:
5/6/2004 Test ID:
CdFRCR Sample ID:
TVA / Sequoyah Nuclear Plant - Intake End Date:
5/13/2004 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Comrnents:
NON-TREATED Cone-%
1 2
3 4
5 6
7 8
9 10 D-Control 30.000 31000 32.000 29.000 28.000 35.000 31.000 36.000 28.000 36.000 100 28.000 31.000 30.000 31.000 35.000 30.000 34.000 34.000 29.000 32.000 Transform: Untransformed 1-Tailed Isotonic Cone-%
Mean N-Mlean Mlean Mln MaY CV%
N t-Stat Critical MSD Mean N-Mean D-Control 31.700 1.0000 31.700 28.000 36.000 9.757 10 31.700 1.0000 100 31.400 0.9905 31.400 28.000 35.000 7.385 10 0.245 1.734 2.120 31.400 0.9905 tj Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates norrnal distribution (p > 0.01) 0.93010801 0.868 0.28577713 -1.1275073 r-Test indicates equal variances (p 0.40) 1.77892566 6.5410A953 hlypothesis Test (1-tail, 0.05)
Homascedastic t Test indicates no signifcant differences Treatments vs D-Control MSDu MSDp MSB MSE F-Prob dr 2.11984895 0.06687221 0.45 7.47222222 0.80891991 1, 18 sqnf_5-06a04data
Environmental Testing Solutions, Inc.
Statistical Analyses Lftn2rlnterpohadon (200 Resznrles)
Sktw PONt SD 95S CL 1c05 ICIO IC1S IC20 1C25 IC40 IC50
>100
>100
>100
>100
>100
>100
>100 1.U 0.9 0.8 0.7 0..*
0.4 0.3 0.2' 0.1*
nno -...-
20 40 60 80 100 120 Dose %
Dose-Respome riot An.
35.
30*
o 25-
- u 20-0
&0.
10 -
5.-
1-tail, 0.05 Iel of sIgniffcance 04 90 8
sqpOS-065-odata
- i
, f 4'.-
(-- '. % -
Environmental T'estinig olutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia duibia Daily Chemical Analyses Client:
TVA I Sequoyal, Nuclear Plant, Nan-treated Test dates:
Ma2y06.13,2004 Project number: 1206 Reviewed by:
Concentration Parameter Day 0 Day I Da 2
11 3 Di4 Day S D~a 6 Initial IFinal Initial IFinal Initial inl Initial inl InitIl Fnal Initial IFinal Initial IFinal nil (SU) 7.681 7.671 8.021 7.661 8.05 7.6 7.82 7.9 7.63 7.9 7.641 7.49 7.491 7.63 DO) (rnVJI.)
7.91 8.0 8.01
- 8. I1 7.9 8.3 8.2
- 8.
.21 8.0 8.01 8.1 Conductivity (tiMboscm) 303 33 28300 287 37302 Control Alkalinity (mgIL C2CO,)
-64 6
62 1hardness (mgIL C2CO,)
82 82 82
_______Temperature
('C) 25.1 244 25.0 24.81 25.2 24.9 25.0 25.1 25.4 25.0 24.8 249 25.1 2.
pit (SU) 7.76 76 7.70 7.65 7.68 7.65 7.69 7.61 7.58 7.69 7.56 7.527.2.4 10.98%
D (MgIL) 7.9
- 8.
8.1 8.2 B.)
8.2 8.3 8.28.
8.2 8.2 8.0 8.8.
Cnductivity (gimhosfcm) 2930276
~
28R8 2173294
_______Temperature
('C) 24.7 243 24.9 24.6 25.0 24.9 25.0 25.0 25.4 25.1 24.8 249 25.0 2.
p11 (SU) 7.74 7.67 7.68, 7.64 7.65 7.64, 7.681 7.62 7.55 7.69 7.54 J7.
8J748J.
3 22% D (mg/L) 8.0 8.2 8.2 82.28.2 8.2 8.3 8.2 8.2 8
J.2 s.0J 4
2% Conductivity (pmhos/cm) 28 27270 281
~
271 24 2~81
_ T meratu re CC) 24.7 24.3 24.9 248 25.0 249 25.0 25.0 25.2 2.249 24.9 25.0 2.
ell (SU) 7.72 7.66 7.67 7.4 762 763 64 761 7.49 76 7.50 7.0 7.44 7.611 49% DO (rnpL) 7.9 8.2 8.2 8.2 822
- 2.
8.3 83 3
8.28.
8.0 8.
Conductivity (pmhoslcni) 262 262 248 252 249 240 239
_______Temperature CC) 24.5
- 2.
24.91 24.7 2501 24.9 25.0 250 22 25 24.8 25.02505.
p11 (SU) 7.71 7.5 7.64 7.6 7.62 7.62 760 60 7.43 7.66 7.471 7.38 7.38 76 7V/2 DO (Mgm) 8.0 8.2 8.2 8.2 8
.2 82 2
84 3
8.3 8.2 788.08.
Conductivity (tzmhos/cm) 226 226 217 21207 209 218
_______Temperature
('C) 24.41 24.2 24.7 24..7 25.1 24.9 24.9 25I 25.2 24.924 825.11 25.1 pit (SU) 7.691 7.63 7.62 7.66 7.61 7.63 7571 760 7.361 7.65 7.43
.7 7.351 7.62 DO0 (niJl.)
7.91 8.2 8.1 8.2 8.3-8.21 831 85 8.31 8.3-8.2 798.01 8.1 Conductivity (prnho~en) 92931890179 17 8
100%
Alkalinity (mgL CaCO,)667 69M Hardness (mg1L Ca CO,)
71 757 Total Rsildual Chlorine (mg/L)_
<.0 01
<0.200 Tmperature (IC) 24.31 24.41 24.61
- 2.
25.31 24.91 25.0 25 1 25.41 25.11 24.81 24.91 25.11 25.11 pi1 (SU) 7.681 7.61 7.61 7.68 7.611 7.601 7.611 7.59 7.711 7.65 7.621 7.361 7.671 7.631 no (mWiL) 8.01 8.01 8.31 82 8.31 8.01 8.41 8.4 8.41 8.4 8.31 8.01 8.21 8.
Conductivity (prnhoskem) 191 1
9 100% I Make Alkalinity (mCII. CaCO,)
60 66 Hardness (mgIL CaCO,)
75~
TotalRe-sidual Chlorine (mtgL)
<0.10 0I<00
_______Temperature
('C)24.7 24.41 24.71 24.8 25.21 24.91 24.91
- 25.
5.-5.
- 4.
- 4.
25.11 25.1
. -
I Iv 4.1*.
I I
p I
I I
b I
I I
Environmental Testing Solutions, Inc.
Page 6 of 7 Species: Ceriodaphnia dubia Client: Senuovah Nuclear Plant - Non-treated Da_
Chemistr.:
Date:
ns--ro-QO4 Dav Analyst.
A Concentration Parameter CONTROL pit(S.U.).Le w.1 G3.oz S3o0Z DO (me/L) 1.9_
I q
Conductivity (timhos/cm) 33Zl__
Alkalinity (mg CaCOAL)
Hardness N
(mg CaCOAL)
Temperature (°C)
ZS. 1 A.4 25.%
2zW 75
.5-a.
4q pHl (S.U.)
-4.1O
.h) 1.L 9tS 10.98%
DO (mg1L)
_7_- ___.
I B-2 Conductivity (pinhos/cm )
__0_
Temperature('C)
ZCi.1 2(A.3 24.9 Lq.(D 5.O Z4 PIT (S.U.) 17
-?. (o qtle
-i-t-A
-I. (0
.to 22%
DO (mg/L) 6 r) 6.iN 1.Z 8.. t
.Z.
3.2 Conductivity (ymhos/cm) 7, 3
j X Temperature (°C)
I Z
3 z_
_14 ZS. O.
_2_4 A pl_(S.U.)
_(_
_O
. (1 1 A 2.
-. L 3 43.9%
DO (mg/L) l P.__R.
Conductivity Z
(pmhos/cm)
Z.OZ..WM__
Temperature ('C)
I.L5 r zZQ.
4.9 v1.,
zS.0 2zq.
p~l- (S.U.)
-71.71
- E05,
- q. (o6 q
.X
--: U(;
?
72%
DO (mg/L)
.Z z
E. L.
Conductivity Temperature (°C)
ZY.
ZL
% *1 21 2S.
Z-.MA PI I(S.U.)
-7 1o L..I o3
-4.(p Z
-I.(
I.D
-0
-q.4e3 100%
DO (mg/L)
I
- 8.
f3.
0-Z ConductivityIt (u+/-mhos/cm)
IQ Z.
Alkalinity (mgz CaCO3IL)
Os Hardness (mg CaCOtIL)
OlO Temperature (°C)
ZY.*3 2'.
Zq.(
- 24. *P X.3 Zi q4 pit (S.U.)
1-b-
-)17-to l
-P I1 e8 7 ol
% o 100% Intake DO (mg/L)
- 8.
D 8Pt 7-
.Z.3 Conductivity Alkalinity MW (mg CaC 3L)
Hardness 13 (mg CaCOIL)
<0 10 L.
I (mgpL) l Temperature (CC) 2q.i7 ZLI.L 2 I. 1
."I zq-Initial Final Initial Final Initial Final I
Environmental Testing Solutions, Inc.
Page 7 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date:
OSc1.pDA cj 9
I Day I
I I_
I 3
I l
Analyst I Concen-Parameter tratlon I
CONTROL pH (S.U.)
I S..
DO (mg/L)
I 6iZ 1 it 4-I-4-I II I
- -f I,
I 6 i : I . ",
ConductivIty (umhos/cm)
.300 rwr__
C2.4-'
- 2197 Alkalinity (PC)
(mg CaCO3/L)
Hardnesr
\\s)1(
(mg CaCOjAL)
.i 30o
&'Z TemperaturecC) 2=5
-7S V
b
- /.0 I
pl (S.U.)
i.s X
7.5w 1052 5 2 10.98%
DO (mglL) 3.2 ao Conductvlity 262
__2 Tem perature rc)
Z5.0
.0Zyq__
pH (S.U.)
Z b 7 (,o 7 7.f;5
?.3 22%
DO (mfL)
_3 _2
,0
_0 Conductivity 261
_l__
3 Temperature (C) 7S5 50 Z:5.
Z/
p1 (S.U.)
1.P
____7 c
43.9%
DO (mg/L)
_B_= __
B3.
R Conductlvlty 25 Temperature (C)
Q O
2Z
.Z?5O.
pH (S.U.)
_.L0
.to oT.
9 72%
DO(mg/L)
AZ 8
- 8.
_3_;
Conductivity Temperature (C)
ZL.j 1
2S.
o
- 2 *
- 2.. /
Z.
p11 (S.U.)
-: 153C 1
3(
. 3 V
155
,Z 100%
DO (mgfL) 8-3 6
-2:
- 6.
8 ConductivIty (prmhoslcmi)
Alkalinity (mg CaCOIL)
Hardness TR Cblorlne (mg1L).
Temperature (C)
_5.0
- 25.
1 B
(
9
- 2.
z.
pH (S.U.)
7._ Lo I
_I 100%
DO(mgIL)
.4 e71l B V 3
B. ° 0
In ta k e Conductivity I
1 /36 (nmihos/cin) 1;3 Alkalinity Hardness TR chlorine (mQIL)
Temperature CC) 5 Initial 11 Fln2l I
Initial I
Final I
Initial I
Final Iinitial" I
l~
....i
.I ni_
I F..a.
I
_i I
Fin.l
Environmental Testing Solutions, Inc.
Page I of 6 Environmental Testing Solutions, Inc.
Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant - UV-treated NPDES X: TN 0026450 Project #:
IZ_ 0, I
I.
I1 iI "
Dilution prepara tion Information:
Comments:
Dilution prep (%/6) 10.98 22 43.9 72 100
. E u
Efluent volume (mL) 274.5 550 1097.5 I00 2500 1ti t
f%
2 Diluentvolume(rnL) 22255 1950 1402.S 700 O
O
-40O ATV Tota volume (mL.)
2500 2500 2500 2500 2300 Aj Test organism information:
Test information:
Organism age:
Z%.S To v4-t%
o>
a oi Randomizing template: B Date and times organisms cjs-oS.c%'4 I1%35 T
14CO KS-C Incubator number:
were born between:
I %!au re t Etc Organism source:
AS 6BA os-s-oq Artemia lot number:
U3rC1oq.
Transfer bowl information:
pH1-Temperature =
C Total drying time:
?4 Voo35 21b -.
Date/Time in: t4l3-cK iq 4
Average transfer volume:
Date / Time out: cS-4
_qs_
Oven temperature:
bl C Dailyfeeding and renewal information:
Day Date Morfnlg Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time IAAS 0
CA-iA Ob-4 cs-as-cSfi oq
'so.z5
.Zq4 A
I oS-o-0 10 160t Gl5 S-M-CA i
OSCG. 3 2 d
.2 I5O-ssi CAS Wb-4 OSOSi Sw 1.D05 3
CS-0c-Oq Mlc4 MGV Z 15 O
LY b-o-stw-ol 1 0-K 4
OS-c -o 1o3 too 1 bq2 OS-c-1U M ec'9.o0 1.o-z 1<
S oS 11 Oc' IOQD 16Oll3Z O-o1-i biZ3',.I I.oz K( I 5
oS-il1o-4 1000 (L.Oq 137.p MSa.cqOA 04 DC~z3. b l. Oz.
KEX<
6 OS-iz-cA 05'i hb IL.
OS-I c-04
,q 05,O I 1.0z KEK 7
A.3t f
-i i -114 r&5tg>i@t
- YsK Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
5 s20%
7-day LC50
> I C 7 /.
Average weight per initial larvae:
nj
'..r
-c
~:
". NOEC I M7 Average weight per surviving larvae:
- 0. MSI1 2 0.25 mnglarvae LOEC
> OC)%
ChV
> 1 007 IC-2 I 5 1o7.
Environmental Testing Solutions, Inc.
Page 2 of 6 Environmental Testing Solutions, Inc.
Page2of6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date:
O-S-o..-OcA I.
I I
I I.
.4*1
.1.
I I
I, ii.
Survival and Growth Data Day CONTROL 10.98%
22%
A B
C D
E F
G H_
I J
K L
O 1
/D
/C)
/0
/0
/0 10 /0
/0
/0 /0 /o 1
/D
/6
/0
/C 10 Io 10 10
/0 /0
/0 40 2
(0 /0 c(0 /6 1
/I
//c Io /0 I
I 3
10
/o
/&
10
/(
/,)
CJo 10
/0 10 10 (0
4
/0 lo IC) lo) Io)
ID
/o.
./0 to 0
10
/0
/1
/0
/6
/6
/6
/
1-o
,o 16 /6
/6
/_
.6 1/
/0 /0 I 0
10 10 /0 1
/0 /0 I
/I0
./
I DC) 7
/1 01 to I
IL)
/o v i o (6
A - Pan weight (mg)
B - Pan + Larvae weight (mg)
I
~
o l
Larvae weight (mg) -. A - B larae(m0 f
)
A%2 i ~
Weight per Initial numberof t
op 0
v\\
-C/Inltialnumberoflarvae 0
O-0' O
0' 0'
0*
0' C
0 '
Average weight per Initial number of larvae (mg) 0.. ;
0. : 1 Calculations and data reviewed:
Environmental Testing Solutions, Inc.
Page 3 of 6 Environmental Testing Solutions, Inc.
Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
M-Cio-0-c Survival and Growth Data Day 43.9%
72%
100%
M N
O P
Q R
S T
U V
W X
0 lbI it) IC0) I0 11to 10
/O
/i
/O 1
J lo to 1o Io /° iI lo
/I0 ) I) 1O I 0t01o 10 //(t o
/
0110 /0-/0
_11
(/
J O 16__O j
A A
3~I 10 10 l
/0 J° i0) 0 10 1()
/(10 10
/
4
/D 1
/
I/
/0 0O /I j /l)
/O
/0
/I
/ O
/O /O 10 A
/0
/O 16 Jo
/I
//
1 6
O
_Io t/
/O I Q
/O
/O
/
- 1.
_O
/
/
/)
/)
A - Pan weight (mg) 4,a0
\\%
k 9
yx
\\d.)
IkA
\\S 14-5
%-j
\\&0 6 AA B - Pan + Larvae weght (mg)
)1i
\\gXtF4)4 Zg;d Larvae weight (mg) - A - B6 Weight per Initial number of a
larvae (mg) q1 \\
\\o'-
OA, O0 Y
Q o~ '4' o
-ClIniltlalnumberotlarvae O'
O O-O0O Ci O-O_
Average weight per Initial number of larvae (mg)
- 0.
0.-
oo Calculations and data reviewed:
Comments:
4 i!--.
Environmental Testing Solutions, Inc.
I-5:1 I.,
Pagc 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date:
CS-O0-(A I:
1 I
i.
4.,.I.
I I
I 4
I I
I is I
Survival and Growth Data Day 100% Intake Y
Z I AA BB 0
/1 10 10 1O 1
- 1)
/10 I0 iL) 2
/0 /0 ) /0 c
3 S_ _
/O
/0
/O ID 6
IC /0 0
/(
7L j
/
/0 A - Pan weight (mg)
B - Pan + Lanat welght (mg)
Larvae welght (mg) - A - B Weight per Initial number of
_O larvae (mg) cl
- C /Initial number oflarvae 0OO Average weight per Initial number of larvae (mg) o,1 Co Calculations and data reviewed:
Comments:
Environmental Testing Solutions, Inc.
Chronic Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pmrephalespromelas Quality Control Verification of Data Entry, Calculatlons and Statistical Analyses Client:
TVA Test dstes:
Stay Project number.
1206 I Sequoyb Ncltar PlMat - UV treated 06-13. 2004 Rcvelwed by:
t1/
_c>
R1.
Jn bt nl" rWf 1-1 A-n rx dtb aii-r...L-.
L-ee tb(V W.tb/-W Witgjjt"hW teS.-
M...
dtb I
C-lf~as -
iS 19 t*sW aw d C
~
r 8 0_
b-e
(-3
_#_b1s 1e%_
(.4
~'
rA A
10 t0 14.66 23.43 1.77 0.877 0.77.
control B
10 10 15.01 22.53 7.23 0.752 0.559 t99 0.752 100.0 0.159 3.9 Not appicstble C
30 90 14.73 24.06 9.33 0.933 0.933 D
0o 30 I5.II 23.35 8.74 0.t74 0.874 10 30 14.43 22.67 324 0.S24 0.t24 10.9h o
30 10 14.03 21.76
.93 0 693 etO3
- 9.
0.693 100.0 0.803 9.2 66 O
t0 I90 95.09 23.57 3.56 0.356 0.356 It to to 14.97 2334 S.37 0.837 0.837 to 10 1 5.04 22.4 1 7.37 0.737 0.737 22%
J t0 30 145.4 22.72 3.l3 0333 0851 191.6 0.83 100.0 0.351 38.6 1.0 L
10 10
_ 1.11 23.87 8.76
_.76 0.876 MW 10 to 14.91 23.42 t.51I O.S51 O.t51 43.9-N I 0 10 9 4.94 23.2 1
8.27 0.827 0.849 4.3 0.827 300.0 0.349 4.1 1.2 o
90 90 14.51 22.73 3.20 0.320 0.820 90 10 15.01 23.93 t.97 0.S97 0.t97 Q
90 10 14.31 24.46 9.65 0.965 0.965 72%
K 1
I 0
I 0 4.76 24.51 9.7 0.975 0.949 2.7 0.975 100.0 0.949
.10.5 S
3 0 3 0 14.75 24.133 9.33 0.938 0.933 1.
T 30 10 14.54 23.72 9.1S 0.919 0.91t U
10 90 14.31 24.A3 9.92 O.W2 O.92 100%
V
._10 I0 15.05 24.64 9.59 0.959 0.908 11.6 0.959 100.0 0.903 11.6
.5.7 W
90 30 14.45
.22.00 7.55 0.735 0.755 X
90 90 14.32 24.09 9.27 0.927 0.927 Y
10 30 14.61 23.57 8.96 0.396 0.896 00%.
1 dake Z tO 10 14.96 23.75 5.79 0.879 0.916 3.3 01.379 100.0 0.916 3.3 4.7 ABA 0
90 14.89 24.49 9.32 0.952 0.952 BB 90 90 14.47 23.35 9.33 0.933 0.933 out n 339:
Duutt's MSD "ato:
PMSD:
Dunnet's MSD tat:
PMSD:
0.1284 14.9 0.0s33 95 MSD -
Minimum Significant Differnce PMSD -
Percent Minimusm Sgnificant Difference PMSD ib meautre oftest precision. The PMSD Is the minimun percent difference betweet the contol and Oratment that caa be declared sastiedally dgmnificnt in a whole effloenl toxicity test. On awenge, a significant difference occurs roe EnvironmentalTesting Solutions. Inc. chronic toxicity tests ven a toxicant reduces pfephotes growth by I5.3% fmta the cootrol (detesnined throngh aferece toxicant testing).
.owerPMSflonoddctleinedbyiUSEPA(1Oth percentile) - 9.4%.
Uppe PMSD botad detesmined by USEPA (90th pecentle) - 35%.
The bweand udppebound twcre calkcnled bythe USEPA sing205 tests conducted from 19 Lboantories forPfmepAes growth i chroniercrrence toxicanttest USEPA. 2000. Usderstandiag and Accotuing foe Method Variability In Whole Effient Toxicity Applications Under the National ?ottlutnt Dileharge Elimlatlon Proman. EPA833l R400.003. US Enviroamental Protection Agency. clnusnd, 0(1I
I v,-.
Environmentai T1esming Solutions, Inc.
S i i I
II
! i. i Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/6/2004 Test ID:
PpFRCR Sample ID:
TVA / Sequoyah Nuclear Plant - Outrall 101 End Date:
5/13/2004 Lab ID:
ETS-Envir. Testing Sol.
Samrple Type:
DMR-Discharge Monitoring Report Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comrrtnts:
UV-TREDATED Cone-%
1 2
3 4
D-Control 0.8770 0.7520 0.9330 0.8740 10.98 0.8240 0.6930.
0.8560 0.8370 22 0.7370 0.8180 0.9710 0.8760 43.9 0.8510 0.8270 0.8200 0.8970 72 0.9650 0.9750 0.9380 0.9180 100 0.9920 0.9590 0.7550 0.9270 Transrorm: Untranstormed I-Tailed Isotonic Cone-%
AMean N-Mfean Mean AiM Alex CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.8590 1.0000 0.8590 0.7520 0.9330 8.885 4
0.8697 1.0000 10.98 0.8025 0.9342 0.8025 0.6930 0.8560 9.243 4
1.061 2.410 0.1284 0.8697 1.0000 22 0.8505 0.9901 0.8505 0.7370 0.9710 11.582 4
0.160 2.410 0.1284 0.8697 1.0000 43.9 0.8488 0.9881 0.8488 0.8200 0.8970 4.100 4
0.192 2.410 0.1284 0.8697 1.0000 72 0.9490 1.1048 0.9490 0.9180 0.9750 2.730 4
-1.689 2.410 0.1284 0.8697 1.0000 100 0.9083 1.0573 0.9083 0.7550 0.9920 11.622 4
-0.925 2.410 0.1284 0.8697 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates nommlu distribution (p>0.01) 0.93476695 0.884
-0.7138201 0.30966581 Bartlett's Test indicates equal variances (p = 0.25) 6.56997252 15.0862722 Hypothesis Test (1.tail, 0.05)
NOEC LOEC ChV TU MSDu MISDp MSB MSE F-Prob df Dunnett'sTest 100
>100 1
0.12838357 0.149457 0.01056997 0.00567564 0.15134475 5, 18 Treatnents vs D-Control sqnf__S-0604data
-.Environmental Testing Soiitions, Inc.
Statistical Analyses Unte2r Interpotztlon (200 Rn2mples)
SD 9S% CIExp) -
Skew Potnt Ices X'do
'C'S 1C20 1C23 1C40 xC50
>100
>100
>100
>100
>100
>100
>100 1.0 0s -
0.8.
0.7-0.6-
°. 0.5-0 0.4-n04 a 0.3 0.2-0.1 0.0
-0.1 AU I.
0 20 40 60 80 100 120 Doso %
Dom-Response Plot 12' Ja 0.8 -
o 0.6 0a t% 0.4 0.2-n -
L.~
1-tal, 0.05 level of signlficwce C
03 o0 at at in 8
sqn_05-06-04dafa
Envifonmental resting Solutions, fnc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/612004 Test ID:
PpFRCR Sample ID:
TVA I Sequoyah Nuclear Plant - Intake End Date:
5/13/2004 Lab ID:
ETS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Protocol:
CHRONIC-RPA-821-R-02-013 Test Species:
PP-Pimephales promelas Comments:
UV-TREATED Conc-%
I 2
3 4
D-Control 0.8770 0.7520 0.9330 0.8740 100 0.8960 0.8790 0.9520 0.9380 Transform: Untransrormed I-Tailed Isotonic Conc-%
Mfean N-Mean Mean Amlin Max CV%
N t-Stat Critical MSD Mean N-Mean D-Control 0.8590 1.0000 0.8590 0.7520 0.9330 8.885 4
0.8876 1.0000 100 0.9163 1.0666 0.9163 0.8790 0.9520 3.754 4
-1.368 1.943 0.0813 0.8876 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilks Test indicates normal distribution (p > 0.01) 0.93401957 0.749
-0.9427791 1.3786742 I-Test indicates equal variances (p = 0.22) 4.92398739 47.4672279 Hypothesis Test (1-tail, 0.05)
NISDu MISDp MfSB MSE F-Prob df lomoscedastic t Test indicates no significant differences 0.08133308 0.09468345 0.00655513 0.00350379 0.22039056 1, 6 Treatments vs D-Control sqn~_05-06-O4data
Environmental Testing Solutions, Inc.
Statistical Analyses LUne2r Inttrpolation (200 Rumples)
Skew Potnt SD 95% CL(Ezp)
IC05 MCIO IC15 1C20 ICS IC40 IC50
>100
>100
>100
>100
>100
>100
>100 1.0 0.9' 0.8.
0.7-0.6.
0a 03.4 0.2.
0.1.
0.0
-0.1.
-02 0
20 40 60 80 100
- 120 Doso %
Dose-Response Plot 0.9 0.8 0.7 t 0.6 2
C 0.5 0 0.4 P.-
0.3 02 0.1 0
. '3 1-tal, 0.05 level of slgnificance e
sqn_05-06-O4data
-I
- Efivi-r-6iiiimetalTcsting Solutions,In; e.;
Chronic Whole Effluent Toxicity Tcst (EPA-821-R-02-013, Method IOOO.O)
Species: Pimephalespromelas
- - -State
,'r. 41.
C
,I II;I ii Daily Chemical Analyses Client:
TVA I Sequoyah Nuclear Plant, UV-Treated Test dates:
May 06 - 13, 2004 Project number 1206 Reviewed by:
Concentration Parameter Day 0 Day I I
Day 2 1
Dry3 Day 4 Day 5 Usy 6 IniCiil IiFinal IInitial IFinal Iinitial l Finl Ignit.al I
FCnl Inital lFinal-I Initll IO Fnl Init)l I Flnal pil (SU) 7.68) 7.661 7.85 759 7.80 7.43 7.63 7.34 7.55 7.35 7.521 7.441 7.495 7.49 1)O (mgtL) 8.01
.8 8.2 7.4 7.9 7.7 8.0 7.7 8.2 7.8 8.2 7.8 8.0 7.9 Conductivity (jImhostem) 300_
Control Allulinity (m<<gf C2CO3)_
lHardness (ffgtL CsCO3)__
ii____
i Temperature (C) 25.4 24.1 25.01 24.4 25.1 24.5 25.0 24.2 24.8 24.5 24.0 24.3 25.2 24.4 pit (SU) 7.69 7.66 7.85 7.59 7.76 7.42 7.63 7.34 7.54 7.36 7.51 7.44 7.50 7.45 2
DO (mnIL) 8.1 8.0 8.2 7.5 7.9 7.6 8.1 7.7 8.2 7.8 8.2 7.7 8.0 7.7
.98
- Conductivity (pmhos/em) 201 298 7
267 7 26 288 271 298
_rmper2ture (C) 25.4 24.1 25.1 24.4 25.1 24.5 25.0 24.1 25.0 24.5 24.9 24.3 25.0 24.4 nit (SUI) 7.69) 764 7.83 7.59 7744 7.62 7.40 7.53 7.39 7.50 7.43 7.49 7.45 DO (nWlL) 8.1 8.0 8.2 7.5 8.0
- 7.
8.2 7.8 8.2 7.8 8.2 7.7 8.0 7.7 Conductivity (umhos/em) 287 63 286 252
_274 1
265 264 272 Temperature ('C) 25.2 24.1 25.1 24.3 25.1 24.5 25.0 242 25.0 24.5 24.9 24.3 25.0 24.4 pil (SU))
7.68 7.5 7.X7 7.56 7.74 7.43 7.5 7
7.48 7.40 7.49 7.45 7.43 7.46 43.9%
DO (mg/L) 8.1 S0 8.2 7.4 8.0 7.7 8.1 7.8 8.2 7.8 8.3 7.7 8.0 7.7 Conductlvitv (I mhos/em) 263 261 4249 249 241 Temperature (C) 25.3
- 24.
25.0 24.4 25.0 24.5 25.0
- 24.
- 2.
25.0 24.4 25.0 24.3 nli (SU) 7.68 7.'
7.79 7.U 7.73 7.45 7.8 74
.7.484 7.48 7.45 7.44 7.49 72o DOmtL.)
8.2 8(
8.1 1A
.0
- 1.
.2 788.3 788.3 7.7 S.0 7.7 Conductivity (umhoe/cm) 226 1228 214 220 214 20 2
Temperast u rre 25.3
- 2.
24.9 243 24.9 245 25.0
- 2.
25.0 245 24.9 244 25.1 24.3 pil (SU) 7.661 7.611 7.701 7.561 7.72
.2 7.55 7.381 7.441 7.381 7.46 7.46 7 0 74 DO (rn-g/L) 8.11 8.t1 X.l 7.61 8.1
- 7.
.1 7.9 8.31 7.81 8.3 778.0 Conduct ivityv (pmhilostem) 19 9
79 1851t1710l 100% e Altklnity (mnfL CaCO,)
Ilardness (ng/l, C2CO,)
Total Residual Chlorine (nVlL)
Temperature 25.4 24.1 25.11
- 24.
24591 24.51 25.01 24.1 25.1 24.5 24.91 24.5 2S.1 24.3 pit (SU) 7.66h 7.621 7.781 7.561 7.691 7.471 7.541 7.441 7.631 7.49J 7.5')
7.451 7.561 75 t)O (mg/L)
.l1 7.91 S. I1 7.41 8.11 7.7i 8.31 8.01 8.41 8.21 8.31 7.61 7.91 7.
C~onductivity (limhostem)191917_ _11li18_1S 100% Intake Alkalinity (nWtl CaCO) ___l_
llardnesi (m#tgL CC)I __
Ttal Residual Choie(4L l
ITemrnerature (IC) 25.4 24.11 25.11 24.3 25.11 24.5l
_ 25.01 24.21 25.21 24.51 25.11 24.31 24.91 2.
IC.
I' I
WC S..
Environmental Testing Solutions, Inc.
Page 5 of 6 Species: Pitnephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Daily Chemistry:
Date:
COS 0-(3-A
_ _ _ _ _ _ _ _ _ _D a v 2
Analyst TIE i1+
Concentration Parameter 4AWf; CONTROL pHl(S.U.)
7.t-1 la 1.S5
=
1 3
1T DO (mg/L) 8.z Conductivity Alkalinity (mg CaCO3}Lj Hardness (mg CaCO3/L)
Temperature( 0C)
.Z5
- 27.
2S.0 214.
2S. I 24.S pIT (S.U.)
-7.(9
-,ae.
1.09 i S9 5
-. 2 10.98%
DO (mgtL)
S8SZ__
Conductivity Temperature (°C) 2 -S A.
25.1 2.l pll (S.U.)
-7.
-1 >5 1P
-3.sO 3
-. n 5Cj
-1t
_~ 2_
22%
DO (mg[L) qi 8 7 1 7.S
- 9.
(
Conductivity 2-52.
(p.+/-mhos/cm )
Temperature (C) 25.'S.
- 2.
. 2 2S
- 4 -3 2
l pIT (S.U.)
-I bq 1.>
.7 I
43.9%
DO (mg/L) 1.1 l
I 5Z2 Conductivity (pm hos/cm) 2Ao(
2 4.
Temperature(t) 2S3
- 2.
2 1S.O Z.43 2g.C o
.5 pH (S.U.)
- 7.
- -7(Q71.
13 4S7 72%
DO (mg/L)
S.Z.
Conductivity 2
2Z{
(iamhos./cm)
__Z 2-6 Temperature (C)
- 25. 3
- 2. l
.9.3
.9 2
pil (S.U.)
'*SOte
-1 to l
'7-7 to
-1. (
- 1.7 ?_
. 1 100%
DO(mg/L) 1I I
1 t I0 Conductivity 5
(pmhos/cm)
I Alkalinity (mg CaCOZL)
Hardness (mg CaCOIL) 0 TR chlorine Temperature (°C) 25.4 2'.
?S.
2I.
2.1.
Zt5 pil (S.U.)
14 740z~ 78
-i.5Cx 5.(n
,4 100% Intake DO (mg/L)
-7. q
_+*69 i
°.
Conductivity Alkalinity (mg CaCO 3IL)
Hardness (mg CaCO/L)
TR chlorine Temperature(°C) 2S I 2 3 lt Initial Final Initial Final Initial Final
Eng
.. Environmental Testing Solutions, Inc.
Page 6 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - UV-treated Date: OS-C-04
- 1 1.
I I
Ii:
I-U II
.1'-
I-I 9
- 1
'3,,
A'
Concen-Parameter tr atio n I
CONTROL p1 (S.U.)
.Lo 13 1.5 1.67-
<tI n4Q DO (mgfL) 0...
Conductivity it (jimhos/cm) 2~
Alkalinity Hardness (mg CaCO/ L)
Temperature(C)
ZS.0 Z.8 Z.5 2S.CO 25-
.'l pH (S.U.)
-__o_ g 1.3 t
-7.51
_fiv_6 10.98%
DO (mglL) 6.1
\\.7
-76 F,7.
'I a4.6 Conductivity 20q.
(pmhos/cm)
Z0U I_
_271 Temperature (C) 250o 2Y-I 2-5.o zL.S 24 2,
- 25. C z2'.i-pH (S.U.)
7-.(o2
-6 1.5 l.5cq
-7.5-D i.4o
-i.q 22%
DO (mglL)
- 7. &
pz
?
_1.1 R_
Conductivity z-4 4 (Vmhos/cm)
G ot_0 TemperatureC) 2-S.0 ZZ.
- 25. 0 SA-s 24.9 ZA 3 2 5. O
.2C p11 (S.U.)
59
.3t
-1.Jl IM
'43 4
L__
43.9%
DO (mglL)
I.. bb 3-z.1
- 6s Conductivity 4ci (gtm hos/cm )
Temperature (°C)
ZS.O 2.ZY.
ZqL.
3 25. -
- 2.
ZS. 0 zX4.A4 p11 (S.U.)
.4_
I
_.49
.44 72%
DO (mg/L) 8
.;3 6.0 1
8.-
Conductivity ZZO
.27-(pmhos/cm)
_____N o
w Temperature (C) 7; 0
.zq.
7
- 25. 0 214.S Z.9%
- 24.
-T I5 p i (S.U.)
3:55 2.3z..1 '*.
J 6
(.t tO 100%
DO (mglL)
.8-3 7i 8-3 1
Conductivity (lzmhos/cm)
Alkalinity (mg CaCO/L)
Hardness (mg CaCO 3IL)
Temperature (°C)
ZCj) 7s.
7q.'S
'/.
2q S 24.3
,p{
(S.U.)
7;5 L L(
-Q1=:
- 7. liq 7.-5q
?4.L1 5 1:510 100%
DO (mg/L)
(p 81.9 Intake 33 P"
ConductivityI (umhos/cm)
'I
_78_8 Alkalinity
=
(mg CaCOVL) w\\
Hardness (mg CaCOVL)
-AgJL
\\
Temperature(°C).
1 2 9/ Z5 2. 2..
214.
l 2q.3 t -
Inillial Final
.Initial I
Final Initial I
Final I Initial Final 3
S.-
I If I
'41.
I.
I:
,1
L I
I Environmental Testin2 Solutions. Inc.
-'.s2..d
@{^
En io m n a Te tn So ui nI c
-Q L
C-,
. Page 15 Page I
of I Total Residual Chlorine
- (EPA Method 330.5)
Matrix: Water, MDL = 0. I 0 mg/L Meter: Accumet Model AR25 pHl/lon Meter Analyst l Date analyzed [.5.W-L54 Iodide reagent:
Ig to,,l Acid reagent: EUR 0 II Calibration:
l l0.10 mZL l
.00 mnglLl Reference standard number I tS6 14-1 I I-Ss Iq Note: For samples with a residual chlorine of > 1.0 mni&L, the calibration ranSc must be adjusted to bracket the chlorine levels of the somples.
Laborator control standard:
Reference standard True value (M)
Measured value (:tV)
% RS - NIV/ TV IO00 number (mgfL)
(mgJL)
(acceptable range - 90 to 110%)
\\
%Sq-1~s 0.530 Itl LV Djplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RlPD - ((S - D) /[(S+D)t2]) x 100 number l
(acceptable range -
- 10%)
.A
.D 4,
~D u p lic a te
- ..j~J Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mefL)
Blank (shouldbe-<O.I0mg/L) v 8t Ot SQS.23 SCDJ l1X e4 -a
.r-4IcAe-q (c.oe'4lt Q0-loIot4.. 02-Mc Qy.
Ccd M cbr ppAAe 3c. OSLe OqOS016.0(0 wa6 t'^
-<:k yc(lt 7 toI X500t c4 tsolo. oS F
i k
1 ss.I L.
V.!
o
,s p&..f I-J.W 0oLLCI<
o o'Aristo(ok MnC (-,,W.e, I:Ano. rk eooo-r cMcSO3O I
~ ~
PDDm 4\\o.C C9 Note: All samples werc analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Reviewed by I e1 Date reviewed F
- SoL-O i 1
Environ mental Testing Solutions, Inc.
Page 1L._
Page 1 of 1 Total Residual Chlorine (EPA Method 330.5)
Q.
A:.;Matrix:
Water, MDL =0.10 mglL Meter: Accumet Model AR25 pH/Ion Meter Analyst Iodidereagent:
Date analyzed loCdot 4.
Acid reagent: l 10-Calibration:
I 0.10mg/.
l I.OO nmg/L fRererence standard number l
ARI M rs i'r I IM q7 l
-Note: Forwsples with aresidual chlorine of> 1.0 mgfL, the calibration raibge must be adjustcd to bracketthe chlorine levels ofthe sapls.
Laboratory control standard:
Reference standard True value (TV)
M Measured value (MV)
% RS - MIV / TV x 100 number (mg/L)
I (ing/L)
(acceptable range -90 to 110%)
4..
INSS 1 0.50 l
0.5Z3 l it(J 7.0 Duplicate sample precision:
-;.Sample Sample ID Sample characteristics Residual chlorine
%/R}'D - [(S - D) /[(S+D)121) x 100 J
num ber
.2
_ _ _ _ _ _ _ccepable rnge -
__10%
)
OqCS-'
Z1 a Ic M
0 Se29 e.,'i';i'.
CA__etLo_____
0.002%~o 1~~~~
>-> -tl...
Duplicate 0>.,6.6..2 6o.4Zo ke'
- ^
- C' Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
Blank (shouldbe <0.m10L) 0.
i'I.SS pA o. O RR Z pCI&eA, NO cOL f
O. 030510C t;-; 6. 00 i4AzcIt i
PAt'.1ef PUCILAeSQ&
_A~r
_O._OO_
is_
- O-oe).2z wAtaewJ C(Dr' PAV N PcU J Q 1iACTlCLeS
'0O. lO41 o gC)51
-04 VIA 1sQN 101 L61ir qe!LQO P<-rtc e
_o.___Z_
b~?o y
~
1iACLifiG I4-r
%JQ0 PAI,T LeS X;i Note: All sanples were analyzed in excess of EPA reconmended holding time (I5 minutes) unless otherwise noted.
Laboratory control standardl:
Reference standard True value (TV)
Mleasured value (NIV)
% RS IV JTV x 100 Re number (mg/L)
(mgL)
(acceptable range -90 to Ito%)
,' SS 171 0.50 Reviewed by Date reviewed I
Environmental Testlng Solutions. Inc.
- 4P.
-1.
Page _l Page I
of_ t Total Residual Chlorine (EPA Mlethod 330.5)
Matrix: Water, MDL = 0.10 mglL Meter: Accumet Model AR25 pH/Ion Meter Analyst [(AAI Date analyzed C65eoi04id lodidereagent: lVIe %Clt, Acid reagent:
I L e 1 1 I
Calibration:
- I
- i 0.lOmglL I.00 mIL Reftrence standard number I j s* 1ji 1.0-S
%CA'L I
Note: Forsamples wih a residual chlorine at>i1.0mgfL, the calibration range must be adjusted to bracket thecchlorine levels ofthe saples.
Laboratory control standard:
Reference standard True value (TV) lMeasured value (INIV)
% RS -
IV /TV x 100 number (mgL)
(me/L)
(acceptable range - 90 to 110%)
I155
.I C-1 0.50 1
0-t51 IOU. Z Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mR/L)
Blank (shouldbe=<0.10meL) i
<S.0 a So&. 03
-rSna\\ \\
Ac\\br
.L c C% -
b aoNOE7O.
l5 IAI eo eo /n2Iat uix0 Ke~,________
Oqo!s o§. 01 Za M f.I jo.COoQ pcq otl 0no0^.05 -6AsS
\\AC O W, Mani 5ti: LL4 c n.Q _-
o O.
i:;sf
,- -tao
-aAScs 4O3b 0Q0iOkO
- COv,
-5 cc 00 1 -0 s
PA NC: A5(la. 0 w
a e i Q
a EP n)
O 1 srce rIS,
nl e noted.
Note: All samples werc analyzed in exc s oftEPA recommended holding timc (1S minutes) unless othetwise noted.
Reviewed by Date reviewed
I.......
I'.l I:..%;
L.1 I-:.
W 1.t-I~s:.
I -1'I Ie..
Environmental Testing Solutions, Inc.
Page sC)
. Page 36of 3 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst A
Date analyzed O-0 Iodide reagent: l Acid reagent:
lI Calibration:
I.-'
- -- -R-L O--ml l.Omg/
[
1:
I 0.1 mz/L.
I 1.00 niza I
Reference standard number I
I V I orze: For samples with a residual chlorine of > 1.0 mg/L., the calibration range must be adjusted to bracket the chlorine levels of the sa.ples.
Dupllcate sample precision:
Sample Simple ID Sample characteristics Residual chlorine
%RPD - ((S - D) /[(S+D)t21) x 100 number I
- (aceeptable range d
- 10%)
604501, 0 s t4 I 10 eo L11t S0 OOOOq,
^.
v..........
qZ8 Duplicate
____O_
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mz/L) r' Blank (shouldbe-<0.l0m gtL) t;
(
O~oS&lO2S G X fIAISQIJ
/dMTAKL I
r 16 S
MQ+/-Oa P'A~T1.leS
_______13 US
.06
'Z.~ ~
~
~
-V S0 NT e-L1A Ae,0 AIXLCO.
-)1I Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (NIV)
% RS MV /TV x 100 number (m:JL)
(mg/L)
(acceptable range -90 to 110%)
leJg M 0.50 a-513
/07.. (o Reviewed by I
Z1
?
Date reviewed l 0
0
I.
.1 Page
- 2.
Paze I
of '
Environmental Testine Solutions. Inc.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst E I
Date analyzed CL 1
..c4 Titrate samples to pH = 4.50 S.U.
Titrant normality and muttiplier determination:
pH of Normality Normality (N) of HISO, pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml NaICOs x 0.05)IE (Nx 5000) 100 ml sample water reference standard ml ml ml
- 0.251E
-Ns5S0
- 4.5 S.U.
number number (E)
(acceptable range - 0.018 - 0.022)
LI.44 IJ4 A -a 1
, ~i~t I 0 O-0 tz--
6 7.b o Laboratory control standard:
Reference True value Sample Alkalinity (MIV)
% RS -
MV/TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOjtL)
(acceptable range (mg CaCOjL)
(ml) ml ml ml 90 to 110%)
1sSIl5 i00 100 rL.3 Z2.o
.7 10.2.
Duplicate sam ple precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO..L) l(S(- D) l(S+Dy211 x I00 number (ml) ml ml ml l (acceptablerange-*10%)
t t4 SS Men v
lo ZL-0 15-2, S-7 to.
S 3
IN7,.1->~
s1-Duplicate D
i AMatric spike recovery:
l Reference Spike value Sample l I
Spike alkalinity (A) standard number (SN) volume Begin End Total Multiplier (mgCaCO/1L) l (mg CaCOALl (ml) ml nml ml l
lb SD too Z6-2.
553 6-t 106 Z.2 8.
Sample alkalinity (B) lMeasured spike value (M) l
% R -
MV ISV x 100 (mg CaCOjIL)
IV - A - B (acceptable range L
u l l(mg CaCOJ/L)
- 75 to 12s%)
Sample measurenments:
Sample volume Begin End Total l Alkalinity Sample number Sample ID l
(ml) nml ml. lml Multiplier (mg CaCOJL) n Sd A
O_-
134M l,
l~z B
l ln l4 l
1 o3s rnvIS OAs Z
A 40.3 W
j,.
D 5 J L Q.L.
- o.
Y WO A
S&
I 2..6 A
. L. ' oI I
Reviewed by:
t r d
l Date reviewed:
Environmental Testing Solutions. Inc.
Page
'I Page
- 2.
of'f 4
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst r 7 7 I
Date analyzed Titrate samples to pH = 4.50 S.U.
Tifraiz nar~n a d
multiplier determination:
pH of Nor.ality Normality (N) or HjSq 4 pH Factor or Multiplier Delonized Titrant check Be-in Enzd
( ml Na2 CO3 x 0.05)/E (NX 50000)/100 ml sample water reference standard ml ml ml
-Nx500 in4.5 S.U.
number number (E)
(aceptabic range -0.018.
Laboratory control standard.
Reference True value Sample Alkalinity (MV)
% RS M
NIV / TV x 100 standard n umber (TV) volume Begin EDnd Totlal MSultipie r (mg CaCO31L)
(Mceptuble rangee (mg CaCOj/L)
(ml) ml ml ml 90 to 110%)
Is
- as 100 100 A.b o.C, Duplicate sam le precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO/4L)
((S - D) I(S+DY)2i) x 100 number (ml) ml ml ml (acCeptable range -
10%)
O MAS t-tt_5
_0 qA \\
4 U5..
tO... lot I.,1 Duplicate D
t_
O 1
a 2 -
Alatrix spike recovery:
Reference Spike value.
Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOjL)
(mg CaCO 3IL)
(ml) ml ml ml l
L lD$l1ilf; lt t5-q l24.9 1%,
l102.
l l
Sample alkalinity (B)
Measured spike value (NIV)
% R -
IV / SV x 100 g(m CaCOj/L)
MIV - A - B (acceptable range l
(mc CaCO3IL)
-75 to 125%)
Sample measzsrements: '
Sample volume Begin End Total Alkalinity, Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOIL)
X5lg&l}ts Flzi 0
ZA4ck sit-6 5
5q l
0-2L l
56Lu'Lo
_33_o_3 3
- 1 4
- l.
l_ _ _
A.
ll 2
- L 2 5-6
()t}t) q 3--
8 2
1.1-
-22.
1
~
-I A.
7 zb 1i.Qz l...
i 2
.c8 ZZ.8 j~q 2\\ C Z
l Ltc)6 Il 2
l l
l~ :t 12s t
ZO O
Itxl.
OZ I 5
I
__ 12A.
II I0 104 O
,0 31 S kf
' -Lo.2 1 (_.-_ I
__ I lo Reviewed by:
De e
d 1
Date reviewed: I (05'7-7Z 6fl
Page Z8 Page 3
of
+
Environmental Testing Solutions, Inc.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed 0
Titrate samples to pH = 4.50 S.U.
Trze,,,
i! duid plier determination:
pH of Normality (N oIH S04 pH Factor or Multiplier Deionized Titrant check Begin 1 _ _ e (5 ml NaICO0 x 0.05)1E (Nx 50000)1 100 ml sample vater reference standard ml ml ml SJV500 4.5 S.U.
number number =_=___
)
(acceplablcranee-0.018-O.
Laboratoy control standard:_
Reference True value Sample Alkalinity(MV) e% RS - MIV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOgjL)
(acceptable range (mg CaCO>L)
(ml) ml mnl ml
-9010110%)
I1 455 t3s 100 100-
- g.
Q9 I0O so.
a4
(.
Duplicate sam le precision:
Sample Alkalinity
%RPD -
Sample Samplc ID volume Begin End Total Multiplier (mg CxCo0/L)
J(S -D)I[(S+D)21) s 100 number (ml) ml ml ml (acceptabl range-10%)
I
~sm Duplicate 7
1 I
AfatrLr spike recovery J
Reference Spike value Sample l Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier
((mg CaCO/L) nme (mg CaCO0L)
(Ml) ml ml ml Sample alkalinity (B)
Measured spike value (NIV) l R
NMV / SV x 100 alka C
iCi(/L)
M s u Are -B (accept ble range I
(mg CaCo,/L) 75 to 125%)
Sample nteasurements:
Sample volume Begin End Total Alkalinity lSample number_l Sample ID (ml) ml ml ml Multiplier (mg CaCOIL) 9tto50-S
-235
- 2) 55t'° Iql.s Is A-i oDS 0-4.
6 i-
% 1(-5 Utz}
- Oqs i.0)2 s 3
1 n
ttt M66 1
l 1tS 1.0tQ l
l l2 O'S5.l I A 1/2..
__l r
oJ4.zzJ 2-4 bo a65c.o ZZ
___C4Q.
z.
tf4OSI.O~
3 IB-Revicwved by:
Date reviewed:
06/17/2004 14:46 4238437080 Environmental Testing Solutions, Inc.
TVA SON PAGE 10/14 Page _aq Page
- _ -Of 44 Alkalinity (EPA Method 310.1)
Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst l (I24 I
Date analyzed I-bL0 i
Titrate samples to pH - 4.50 S.U.
pH of Normality
-I.
Normatlity QiV) of H2504 pH Factor or Multiplier water reference standard ml nit ml 2
flN1
%500
=4.5 S.U.
number number us
=
0e22 Laborat control standard:
Reference True value Sample Alkalinity (MV)
% RS -V
/ TV I 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO/L)
(aceepxable range
- Ot cCOCA (ml) ml rnl tax 0 to 1 0°A) s~
100 100 *a s Z&3 g
^
tool Duplicate sam le precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mz CaCO,IL)
IUS - T) II(S+D)flJ) x 100 number (mI) ml ml ml (acceptable range = i 10%)
Duplicate 4
D Matrix spike recovery:
Reference Spike value Sample l F"
lSpike alkalinity (A)
Standard number (SV) volume Begin l End Total Multiplier
( mg CCO-/L).
(m CaCO,/L) 4 Jii ml _
al ml l
l)SS is S
SDM cmC -Z',v151az 1Z '.-_I3 Sample alkalinity (B)
Measured spike value (MV)j
% R =MV / SV 100 (mg CaCOlIL)
MVrnA B (acceptable range (me Cacol/L)
-75 to 126%/)
Sample meatsurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID
(
-)
ml I ml ml lMultilir (mg CaCOVL)
!~bq 6%A efo\\kw;-Ro
(>ah7lt l23"S 1,1.4 I-q-107 I I
IGS\\oqs I fI 1 s a 1
-4) 1 ar I
50-
.1' I
bA I
IT4A FS
-**15°l!
o a3
\\1-l A-z 3
-l Wage l
0 q s.xol. '
IMI f-.4C I
I l3 s>00 1
4 1qg.>1 27' n3 UA_
.)-$
13i~ll.
l 15t) 0
._ I
_I I
Z I
I I
Rcviewed by:
I A---
Date reviewed: lS.
tj o4 I
Page Z Z Page t of 4
Environmental Testing Solutions, Inc.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO/L Analyst l A l
Date analyzed Tirrant normality and multiplier determination:
Titrant Normality check Begin End Total Normality (N) orEDTA pH Factor or Multiplier reference standard ml ml ml
- 0.21E (Nx 50000Y 50 ml sample number number E)
(acceptable range -0.018-0.022)
-NIs 1000 L'4(.l5W5 IllZ l.0 QI-08 t.Z-o2 LA Laboratory control standard:
Reference True value Sample Hardness (hMV)
% RS M hlV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO.L)
(acctptable range (mg CCOAL)
(ml) ml ml ml
- 90 to 110%)
M'S S % tot 40 50 e
%,9
\\-S Z,.C 2,0 -qi ot.%- It Duplicate sample precision:
Sample Hardness
%RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaiCOlL)
((S - D) lI(S+DI)2l x 100 number (ml) ml ml ml (acceptble range -10%)
D05 -6) o SSW A2( 0 6
,-a I t. 2:z.7 2?
L...
s Duplicate V
t4-o D
AMatrL spike recoveri:
Reference Spike value Sample I
Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOIL)
(mg CaCOVL)
(ml) ml lml lmll lIsste lqb
-15 G-k lV-t8
- t@
4§ Sample hardness (B)
Measured spike value (MV)
% R - MIV/SV x 100 (mg CaCO,/L)
IV - A - B I
(acceptable range (mg CaCOVL)
- 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) lml ml ml l
lilpler (mg CaCOA)
Blank l
?1 l (shouldbe-0mgCaCO/L) l 0
°-Co ZrZi l
54 Rlz-6 A i
20.2.
1 LL.-.
l__ t lLisl__
_-O______rAVL ogLo A
I Iz 1 24.1. 14.o lz.
-1 l Z4L. la1tl 4.l5 8B liJ 9-2 ll l
1zs~r l8-!5 l 4*
l Z.
E M AS
-1,u2 A
3 1 a...
4'0 BZ-
- . fO I
____I__
__l G_ll_____w4 lI 4-Z l
___810
~ L '
I ~ ~ t~,
Q, ~ ~
2 i
'I Note: ir >luml ortitrant is used. sample must be diluted.
by:
Reviewed Date reviewed
-I
Page C5 Environmental Testing Solutions, Inc.
Page Z
of+/-.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO/L Analyst Date analyzed l 05i1 iIIIJ ura-otity and multiplier determination:
Titrant Normality e
End Tota1 Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml
- 02/E I
(N x 50000)/ S0 mlsample number number E) 0.018 0.022) 1 Nx 1000 I
Laboratory control standard:
Reference True value Sample l Hardness (MIV)
% RS - MV/TV xI00 standard number (TV) volume Begin End Total Multiplier (mg CaCOj/L)
(acceptable range (mg CaCOtL)
(ml) ml ml ml
-90 to 110%)
I-s 5\\(
40 50 s.C 5.t Z.o C)
\\
0-J Duplicate sam ple precision:
Sample Hardness
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL)
((S -D) Il(S+D)/21) s 100 number (ml) ml ml ml (acceptable range -
I 0%)
PAWyvn?
Pa.
lso 5.0 1-4 zs-
_._-.__,4_
_s,._,:
Duplicate 4
c D
AMatrix spik-e recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin lEnd lTothl l Multiplier lM C2COA)
(mg CaCO,/L)
(m) ml ml ml
%?.s k lot:
bZ t-1
\\
k 1v1 -
Zt.6l
-q Z2 Sample hardness (B) lISeasured spike value(V)
I
% R MV/SVx 100 l
(mg CaCOJL)
IV A - B (acceptable range (mg C2COJL) 75 to 125%)
Samplen easurements:
Samplevolume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mtg CaCOIL) l
§ ~~Blank jS I
l (~~s~hould be -
mg CaCO~xL)
II I
I I
I 0-11
>,l
+
1 O-SA 1,>-
V k l
\\
z oqOs__0-_0L_
CA kz..o L.o
____s_
,_ 12 pqZ CSC.?.
I%
1 I1 l 4-to sl I
S.1 O0 a
1 OSO -to 1
2.4.b 8.3 37 1 oI
°qO5CJ l 4 3
I 3 -ll~5Sl.
\\
l 5
-405O t
-S1L
-Z 1
__-Jw eIIl-b IIS Note: Ir >i 1ml orftitratlt is used. sample must be diluted.
Reviewed I
V119 -
by:
I l Date reviewed l z
1
Page 2 4 Page 3
of Environmental Testing Solutions. Inc..
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyzed kS l4 IJ Titrant Normality check Begin End
-t
-aLrmaity (N) of EDTA pH Factor or Multiplier reference standard fl ml
-ml (Nt s50000)/50 ml sample number number (E)
(acceptable range - 0.018 -0.0 Nx 1000 Laboratory control standard:
Reference True value Sample Hardness (MV)
% RS - MV / TV x 100 standard number (TV) volume Begln End Total Multiplier (mg CaCOJA)
(acceptable range (mg CaCOAL)
(ml) ml ml ml
- 90 to 110%)
z tS S
\\ P <:M 4 0 50 -
- 1.
ne
.o Sa4 q1zo s1 Duplicate sam le precision:
Sample Hardness
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L)
((S - D) 1[(S+D)t21 x 100 number (ml) ml ml ml (acceptable range-10%)
iCO9.
0 Duplicate D
tutrLr spike recover Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mgCaCO)IL)
(me CaCOL) l m)l_ml ml mll 155 Zs 4°i I
1 r
- n.
l 2
l l IS>B 5 1 I ZpA 1
,T4 Sample hardness (B)
Measured spike value (ININ)
@oR NIV ISV x 100 l
(Mg C2COjlL) l INIV-=A -1B (acceptable range (mg CaCO/L)
- 75 to 125%)
Samnple measuerements:
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOAL Blank___
l___*___
l_ (s ouldbe-Om~CaCO9L)
_ _ _--l LOq nq.COs Pl> ;
ea I0: 1 I%.
13 1 \\\\I Z
I6 4 o g.
R
-I z 1 23 13
-r4 U1 1
1 1 qOqsg.oS Y1 AJ16 3
3 3Z.
iŽs2L
).L.
Z IC4K Y4 -,U. O 0 0
~
t 153-2. vm
- 15. 1 1 \\6
- 2.
I4oY5l.b1 1
3 as I I_______q1 I
tqO!5j I I
P I
I n1Q5.D3hO llnE'l~
..al 1 8 31Ž.. 8.
__ III 1
l io~ojJ.D(0 TtA-Jcr l
.A l Q4s l___
Z.O1 Note: If >lSml of titrant is used, sample must bc diluted.
Reviewed by:
I viv- -1 Date reviewed E
06/17/2004 14:46 4238437080 TVA SQN PAGE 14/14 Page i;
Page 4
of_ 4 Environmental Testing Solutlons, Inc.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst l Li.
Date analyzedl 5'
t' Trnt ini ___._
Titrnut Normallty chtek Begin End Total f
o ofEI)TA pH Factor or Multiplier refereace standard ml 01ml 0
7=1 (NfX 50000)/ S0 mlsnmple number number (E)0cetbl sg
.018 - 0.0t2)
= Nx 1000 l1..l.n D
v.rmnt. nrae{Aa-Simple Hardness
%RPD =
Sample Sample ED volume Bcgin End Total Multiplier (mg CaCOJtL)
((S - D) /((S+D)/y21J 100 number (Ml) ml ml ml (necepthble range mt 10%)
U i
=
Duplicate
_DL..
Afatri spike recovery:
RerurwAce Spike value Sample Splke.hardness (A) standard number (SV) volume Begin End Totsl Multiplier (mg CaCO5L)
(mt C&CO31L)
(ml) ml ml ml Sample hardneis (B)
Measured spike value (MV)
% R = MV / S X 100 (mig COCOA)
MV = A - B (acceptable range (mg CaCO3/L)
- 75 to 125%)
Sampne measurements:__
I1-Sample volume Begin End Total Hardness ISmple number Is.mge ID m(l) l l
ml ml Multiplier (mg CRCQJ/L)
Blank l ____________
l should be 0 mg C CO2iL) l
__l_l I0
- 1
.0t I.
4Z I
I' 4
W Note: If >1 5ml 0critfrfl is used. sample muslt be diluted.
by:
Reviewed dl Date reviewed L s iI-IIIII
Sequoyah Nuclear Plant Biomonitoring May 6 - 13, 2004 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 0.7 0.6 State and USEPA Mandated Control Limits Limits Set According to ' 2 Standard Deviations l
l I _I I
g P-0.5 t:
0.4 q-4 1.0 U
P-b 0.9 to 0.8
- ~
~
0.7 0.6 0.5 0.4
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I {
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USEPA Warning and Control Limits Limits Set According to 1 ah and 2 5'h Percentile CVs O",
5 r"'
%%-"" ~V-"
~N-" Tes da
%V 05 tts,\\ tv3 o% on toXe Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
-Warning Limits (mean IC25 4 SA10)
.Control Limits (mean IC25 Z S..2 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference ToXIcant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water Test rutber Tatdate 7-daylCu CT (el.KCI)
(#LY.Cl)
State and USEPA Laboratory Laboratory S
Control ULmts SA.M Warlng Limits SAS Control LbMts Cr-2S CT+2S CT-S,,, CT+Ss,.,,
CT-SA.s Cr + SAM USEPA SAn Warning lhdta CT -S Cr +iSA.
USEPA SAJM Control ULits CT - all CT + SA-Cv I
2 3
A 5
6 7
8 9
10 it 22 13 14 is 16 27 1s 19 20 07-15-03 08-05-03 0O-12-03 08-19403 10-07403 10-21403 11-04403 11-11403 11-11403 12-093 01-06-04 01-13-04 01.20-04 02-03-04 03-02.04 03-16.04 04-06-04 04-1344 04-20-04 0546004 0.65 0.53 0.67 0.55 0.61 0.54 0.63 0.53 0.53 0.57 0.66 0.64 0.68 0.66 0.66 0.64 0.60 0.64 0.63 0.63 0.59 0.62 0.60 0.61 0.59 0.60 0.59 0.5t 0.58 0.59 0.59 0.60 0.60 0.61 0.61 0.61 0.61 0.61 0.61 0.08 0.42 0.76 0.07 0.08 0.47 0.77 0.07 0.07 0.46 0.74 0.07 0.06 0.48 0.73 0.07 0.06 0.47 0.72 0.07 0.06 0.48 0.71 0.07 0.06 0.47 0.71 0.07 0.06 0.47 0.70 0.07 0.05 0.47 0.69 0.07 0.06 0.48 0.70 0.07 0.06 0.48 0.71 0.07 0.06 0.48 0.72 0.07 0.06 0.49 0.72 0.07 0.06 0.49 0.72 0.07 0.06 0.50 0.72 0.07 0.05 0.50 0.72 0.07 0.05 0.50 0.72 0.07 0.05 0.51 0.72 0.07 0.05 0.51 0.72 0.07 0.52 Q.55 0.53 0.53 0.52 0.53 0.52 0.51 0.51 0.52 0.52 0.53 0.53 0.54 0.54 0.54 0.54 0.54 0.54 0.66 0.69 0.68 0.68 0.67 0.67 0.66 0.65 0.65 0.66 0.66 0.67 0.68 0.68 0.68 0.68 0.68 0.69 0.69 0.12 013 0.13 0.13 0.12 0.13 0.12 0.12 0.12 0.12 0.12 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.47 0.49 0,48 0.48 0.47 0.47 0.47 0.46 0.46 0.47 0.47 0.47 0.48 0.48 0.4S 0.48 0.48 0.48 0.48 0.72 0.75 0.73 0.73 0.72 0.72 0.71 0.71 0.71 0.71 0.72 0.73 0.73 0.74 0.74 0.74 0.74 0.74 0.74 0.23 0.24 0.23 0.23 0.23 0.23 0.22 0.22 0.22 0.22 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.23 0.37 0.38 0.37 0.38 0.37 0.37 0.37 0.36 0.36 0.37 0.37 0.37 0.37 0.38 0.38 0.38 0.3S 038 0.38 0.82 0.86 0.83 0.84 0.82 0.83 0.82 0.81 0.80 0,81 0.82 0.83 0.83 0.U4 0.84 0.8 0.U4 0.Q4 0.85 0.27 0.28 0.27 0.27 0.27 0.27 0.27 0.26 0.26 o0.27 0.27 0.27 0.27 0.27 0.27.
0.27 0.28 0.28 0.28 0.33 0.34 0.33 0.33 0.33 0.33 0.32 0.32 0.32 0.32 0.33 0.33 0.33 0.33 0.34 0.34 0.34 034 0.34 0.Q6 0.14 0.90 0.12 0.87 0.12 0.88 0.10 0.86 0.10 0.87 0410 0.86 0.10 0.85 0.10 0.85 0.09 0.86 0.10 0.86 0.09 0.87 0.10 0.88 0.10 0.88 0.10 0.88 0.09 0.88 0.09 0.S9 0.09 0.89 0.09 0.89 0.0Q oesc.
71d IC1, - 74ay 25%
bhnbftnn eCnenatratL An estknatkm ofthe cone ntratin ofpotaumim chbde that would cause a 25% reduction ns Pimephales Voswh for the test pVutinm.
CT Crentraltendency(mean rIC,).
S - Standard devatin ofthe IC2 vabes.
lAboratery Central and WaT ni Ltmits Lobontrycontroland wamghis were stablishedusisgthedtndard devsati ofthe KCsvahinescoespondingto the 10th nd 25th Ircnt& CV.
These rae ere nore dristenl thin the coabul andnghnks recommended by USEPA for the test meth od and endpoint.
S,,-
Stndard dcegtinn apondkigto the 10' perentile CV. (S 1, -. 0.12)
SA Standard devan eomsreponding to the 25' percentie CV. (SA - 0.21)
USEPA Control and Warnting Umits S.s -
Standard devition eoeapondlegto the 75' permentle CV. (S,., -0.38)
S..,,- Standard deviton cofspondhi5to the 90' pentie CV. (S 0-.45)
CV - Cnefrelnt of wthn orthe ici, vlevs UstA. 233 thdmtndmg andAeoti tftwMd Vul.balykWhole MEboiuToaty Apprtbmt d t
iiPO~taDrctse nutLm PrXOpm. 2 R.
.JCn Ofg..sM o is A'cJBeS 't.1.h
H **'Environmeiital Testing Solutions, Inc.
Potassium Chloride Chronic Rcfcrence Toxicant Control Chart for Puineph ales promelas using Moderately Hard Synthetic Water 1.0 0.9 1.0 1-1 s
r-s -j
-ffi-1 ---
z-
-x --r 0.8 U
0.7....
0.3 7
s-0.6-114 0.5 0.4 0.3 0.2 I
Test date 7-day rC25= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimeplhales growth for the test population.
Central Tendency (mean IC25)
Laboratory Warning Limits (mean IC2. + SA.IO, SA=10
- 0. 12)
....... LUboratory Control Limits (mean IC2 :i SA.2S SA.2 0.21)
JUSEPA Warning Limits (mean IC25 + SA75.SA 7S 0.38)
USEPA Control Limits (mean IC2 5,
.A90
_AS
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control efian Survival Growth CT Cv CT MSD PhISD CT for Control Growth
(.)
(mg'e)
(mgcave) for Control Growth CV (%/.)
(C/)
for PMSD (I/)
1 2
3 4
5 6
7 89 10 11 12 13 14 is 16 17 18 19 20 07-15-03 08-05-03 08-12-03 08-19-03 10-07-03 10-21-03 11-04-03 11-11-03 11-11-03 12-09-03 01-06-04 01-13-04 01-20-04 02-03-04 03-02-04 03-16-04 04-06-04 04-13-04 04-20-04 05-06-04 100 97.5 100 100 97.5 100 100 100 100 100 97.5 100 100 100 97.5 100 100 100 100 100 0.910 1.092 0.693 0.745 0.762 0.803 0.895 0.895 0.953 0.744 0.816 0.807 0.770 0.847 0.566 0.686 0.708 0.780 0.784 0.832 1.001 0.898 0.860 0.840 0.834 0.843 0.849 0.861 0.849 0.846 0.843 0.837 0.838 0.820 0.811 0.805 0.804 0.803 0.804 6.7 6.5 9.7 2.9 20.0 5.7 7.4 7.5 11.6 2.2 4.6 3.3 16.5 5.2 4.8 7.3 12.0 9.8 5.5 5.5 6.6 7.6 6.4 9.1 8.6 8.4 8.3 8.7 8.0 7.7 7.3 8.0 7.8 7.6 7.6 7,9 8.0 7.8 7.7 0.12 0.13 0.20 0.08 0.18 0.13 0.10 0.12 0.20 0.18 0.13
- 0.10 0.13 0.11 0.08 0.08 0.14 0.11 0.08 0.10 13.7 11.8 28.2 10.7 23.1 16.4 11.6 13.6 21.3 24.2 15.5 12.5 17.0 13.0 14.8 12.2 19.3 13.9 10.2 12.2 12.7 17.9 16.1 17.5 17.3 16.5 16.1 16.7 17.5 17.3 16.9 16.9 16.6 16.5 16.2 16.4 16.3 15.9 15.8 Note:
CV-Coefficient of variation for control growth.
On avecage, the CV for control growth is 7.7% in Environmental Testing Solutions, Inc. Pimephates chronic toxicity tests.
Lower CV bound determined by USEPA (I0e percentile) - 3.5%.
Upper CV bound determined by USEPA (90e percentile) - 20%
MSD - Minimum Significant Difference PNISD - Percent Minimum Significant Difference PISD is a measure oftest precision. The P:SD is the mininum percent difference between the control and treatrnent that canbe declared statistically significant in a whole effluent toxicity tet. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growthby 15.S% From the control Lower PMSD bound determined by USEPA (I0^ petcentile) - 9.4%.
Upper P'MSD bound determined by USEPA (90' percentile) - 35%.
CT - Central Tendancy (mean Control rowth. CV, or PMSD)
The lower and upperbounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephaler growth in chronic reference toxicant tests.
USEPA. 2000. Understanding an Accounting for Method Vanability in Whole EffluentToxicity Applications Under the National Pollutant Discharge EtininationProgr EPA-S33-R.00-
- 3. US Environmental Protection Agency. Cincinnati. Off.
Organisms obtainedfrom Aquatic BloSystems, Inc.
OS-06-04
Environmental Testing Solutions, Inc.
Pimnephalespromnelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 1.25 C.)
0 e C..)
C; U
1.00 0.75 0.50 0.25 40 30 20 10 0
- I I
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Minimum Acceptance Criteria (0.25 mg per surviving larvae)
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North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
Test date Control Growth or Coefficient of Variation (CV)
Central Tendency (mean Control Growth or CV)
. Control Limits (mean Control Growth or CV 4 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Piniephales promelas using Moderately Hard Synthetic Water sI I
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40 -
30 0
co oo 20 10 0
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Test date
-4 PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean PMSD)
Lower and Upper PMSD Bounds Lower PMSD Bound (10' percentile) = 9.4%, Upper PMSD Bound (90' percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)
-1 I.
Environmental Testing Solutions, Inc.
Page I of 5 EniometlTetn Souin, Ic ae1o Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pintephalesprom elas Jim PI Sum n e Sumner.....
i.W..
PpKCICR Test Number: 32 Dilution preparation information: -
Comments:
KCI CHM number:
N ty
_2 Stock preparation:
50 g KCUL: Dissolve 50 g KCI in l-L Deionized water Diutuon prep (mglL) 300 450 600 750 900 Stockvolume (rnL) 6 9
12 15 IS Diluent volume (mL) 994 991 988 985 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:
Test information:
Organism age:
Z S i-ts 7-S-lAo%3AS.
0oi&
Randomizing template:
lZej-1, Date and times organisms 01S-cy-tmr -r stt0t nS-r Incubator number and F
were born between:
lto SST) shelf location:
Organism source:
A6S 6A-rtt.cX 0S-o0-c' Artemia lot number:
tr3o0 Transfer bowl information:
pH =
SU Temperature =
°C Total drying time:
Z*oAfS
-1i.-(5 2NZ Date / Time in: cS-1-ht
\\l-(-
Average transfer volume:
c Date / Time out: s-LN-F 1N456 Oven temperature:
I____IC_
Dailyfeeding and renewal information:
Day Date Morning Afternoon Test initiation, Control water Analyst feeding feeding time renewal, or batch used time termination time MV1V 0
0s5A-~--
O <3S-Ok,-G PA6t1 19 CS2*(
A os M-cnOA 1sop
_______6N
'.10 o.Usc-3( A 2
mo-c04A lcooo Ibos 1451 OS-CS-X 3
OS OA O4 OWA~
I lotb lLlZ z
OS-CS-0 Y~ EY' 4
AS-IoO-L 1bO3 b1O IC) OIL 30 OS_
YE S
M 0-1\\-o)<
10o00 IbWi 14 \\t 'A 0-iD KEK 6
C slz-OLJ L
M I o I1 1_2.
OS-1cA4
?ZEK 7 y5.13-Oq, I
_I.
_E Control information:
Acceptance criteria Summar oftest endpoints:
I
% Mortality:
(7, s 20%
7-day LC5o 135.t4 1
Average weight per initial larvae:
eg. 2 5.
NOEC 14 £ o Average weight per surviving larvae:
- 0. R 52-2 0.25 mgilarvac LOEC (D On ChV IC25 j C K5I.1
Environrmnen~tal Testiniz Solutions. Inc.
Page 2 of S Environi nental Testin2 Solutions. Inc.
Page2oCS Species: Pimephalespromelas PpKCICR Test Number: 57-Survival and Growth Data Day CONTROL l
300 mg KCIIL 450 mg KCUL A
B C
D E
F G.
H I
J K
L 0/O O
1 Ol/
o 1
o 10/0
/
lO 10 (6 /0
/0
/0
/0 (0
/
/0) lo
/0 lO 2
M/
10/_
lo 016 1_/01/0o 3
1I IC 0
)
/10/
A I
a o
41t 10 10 10
/D 10 10 lo
/O
'V
/0
/0 5
1
/I I t
/6 loo 0
/°0 _
/0 10 7
to IL) to()
lu t
j 1
10 A - Pan weight (mg)
B - Pan + Larvae weight (mg) 0 40 C-Larvae weight (mg) o
-A-B 5
'VcP$
e Weight per Initial number of K @
larvae (mg)NO
-C / Initial numberorlarvae O
O O'
0 Average weight per Initial number of larvae (mg)
Calculations and data reviewed:
Comments:
II v..l
.I,.
II.
Environmental Testing Solutions, Inc; Page 3 of 5 Environmental Testing Solutions, Inc
Pge3of5 Species: Pimephalespromelas PpKCICR Test Number: 352 Conmnents:
Environmental Testing Solutions, Inc.
Chronic IVhole EMuent Tosicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Plmephalespromelas Qu2lity Control Verifcatlon of Data Entry, C21Culatlons, and Statistical Analyses Tet samber PpVKCR
- 72 (132 at 351 Depot St)
Tet data:
Mays-13.2004 Rne-ed bp e>j C...
(.3WL
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c
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- A
.9 So£f43 b-
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a
£.d
(%I
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t.l A
10 10
£1496 23.30 8.340 0 134 0534 a
_____C 10 I o 14 97 2326 a 290 0 t29 0332 5.5 029 77IO.0 0.e32 R5 Ya *t5bk C
to
£0 Is 0) 22.77 7.770 0.777 0 773 D
10 10 14.76 2361 6 890 0O39 0.U39 E
go
£0 14.94 22.92 7.980 0.)96 0795 lflO F
£
£ tO 4 64 2304 a 400 0 340 0.749 14.1 0 40 800.0 0.749 14.1
£0.0 o
to 0-
£4 90 21039 3 990 039 0399 H4 10
£0 14.77 22.35 7.5t0 0.75t 0.73S5
£ 9
£4.92
-23.37 3 430 0 0939 03943 to__
£433 22.99 3170 09WS 0843 11.1 03£17 95.0 0.797 St14 K
10 to 14 8i 22 46 7.590 0.7S9 0.7S99 L
40
£0
£4 6S 22.33
?.630 0.768 7 8_______
L3 tO 9
15.23 22.42 7.400 0793 0.714 too 4
£0 9
t5.25 22.05 6300 0.756 0753 4.0 0630 9LB 0.69 4.2 It to£ 9
£3 01 21.63 6 630 0.737 0663 PJ 1£0
£0
£ 22sl 7.250 072S 0.725S
£ 0 4
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£2382 3 ££0 0.113 0 31£ 75t tO 6
£32 2 033 t
5.320 0 3t?
0 302 13.6 O332 471 0.57 3 t.7 535 S
£0 5
£4.93 19 4£ 4.430 0136 0 443 ET
£0 4
17.06
£769 2630 0 653 0263 IO tO 0
000 0 00 0OOU)0 0000 0O000 V
£0 4.79 I£ 36 1£070 4070 0691 68.5 0°101
_3 0332 71.9
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£0 21
£46s
£6.27
£.620 0340 0)162 0
2 14419 16.66 1.770 0333s I__
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04177 D.wts MsD V.I."
rMsD:
0.13£4
£8II MSr) -
M6 iiOmum Sg3 m
hiot rhffCveCe
?MSD -
Pncawt Mftimfu Sit>e;sant Dittmnor
?MSD III 30 0 pCtdio0. The PMSD i thS miipmpo itdif ence btww
£t6 0ntl8 d
- 8umcnt, C3be de d
it. csyst 130i8c t 3i ole eflcuen btoxicftdy On rver.
S ti5cant iffe enca oeeum for Envi4vometa3t Testiag Soluuions. Inc dcroic toxicity testa wn a to-ant tedo& PbAdales tm k by I 5.1 I d e cntol (de nd oh 40rm = toxicant £ntingt Loan MSD bound Mamlooned byUSEPA (£tth pernuleo-.9 4%
Upper FMS bound detmined by USPA (90Mh percentite) - 3$'/.
The Iowa end 3pperbowid were caiculgod by the USEPA using 209 twu coniduced Ieo 19 laboratoies for tephalaes tpor k to clumak rcrence Intowicarute USEPA 2000.UmdAmdirg w KAcomtlng for Method VaiabhyinWholEtifuetToxt Apptictions Underthe Nbudu Po30at0 Dichxrgel mfknh m EPA-433-R.
- 3. US Etmunt ProtetionaAgn.Cctn4d OIL
£hy,"haur.bfsyrse f s
- u. IAe.,
0546-04
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:
5/6/2004 Test ID:
PpKCICR Sample ID:
REF-Ref Toxicant End Date:
5/13/2004 Lab ID:
ETS.Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
PP-Pimephales prorelas Comnments:
Cone-mg/L 1
2 3
4 D-Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000 1.0000 450 0.9000 0.9000 1.0000 1.0000 600 0.9000 0.9000 0.9000 1.0000 750 0.4000 0.6000 0.5000 0.4000 900 0.0000 0.1000 0.2000 0.2000 Transform: Arcsin Square Root Rank 1-Tailed Number Total Cone-mg/L Mlean N-Mlean AMean Alin May CV%
N Sum Critical Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4
18.00 10.00 0
40 450 0.9500 0.9500 1.3305 1.2490 1.4120 7.072 4
14.00 10.00 2
40 600 0.9250 0.9250 1.2898 1.2490 1.4120 6.318 4
12.00 10.00 3
40
- 750 0.4750 0.4750 0.7602 0.6847 0.8861 12.679 4
10.00 10.00 21 40
- 900 0.1250 0.1250 0.3520 0.1588 0.4636 41.232 4
10.00 10.00 35 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilkls Test indicates nornml distribution (p > 0.01) 0.92857897 0.884
-0.1721673 0.40024071 Equality of variance cannot be confined Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU Steels Many-One Rank Test 600 750 670.820393 Treatments vs D-Control Organisms obtainedfrom Aquatic MioSystems. Inc 05-06-04
Environmcntal Tcsting Solutions, Inc.
Statistical Analyses Panwimter Value SE 95% FiducIldUrmts Sbpc 10.7818442 2.67549798 2.26721575 19.2964727 Intercept
- 25.906615 7.65442751
- 50.266479
- 1.546871 TSCR Point Probits nWIL 95% FlducW Limits ELNJI 2.674 447.489444 71.403081 568.981727 EC05 3.355 517.59535S 141.027223 62J.332242 ECIO 3.718 559.353165 201.744515 659.152452 ECIS 3.964 589.414001 255.858799 686.44253S EC20 4.158 614.453445 307.799656 711.799813 EC25 4.326 636.781119 358.999996 737.750326 EC40 4.747 696.707575 507.954201 S40.925223 ECSO 5.000 735.441443 593.056733 960.193138 EC60 5.253 776.328752 658.585098 1152.69887 EC75 5.674 849.387999 733.427397 1669.35492 EC8o 5.U42 880.252399 757.167534 1954.75218 CC85 6.036 917.647234 782.852167 2358.U32 EC90 6.282 966.963508 813.449247 2997.72321 EC95 6.645 1044.97479 857.247706 4296.2007 EC99 7.326 1208.68584 939.202499 8498.36393 Signirant hetcrneity detected (p - 3.00B.02)
M*Amm L1lcslhood.Probit Control ChlSq Critial 0
9.03329033 7.81472778 P-vclu 3.05.02 5It Siptms 2.8665481 0.09274851 Ittr 4.
0.90 0.8e 0.7-
- 0.6 C
I 0 n.
M0.4 -
0.3 0.2-0.1-10 100 Dose mg/I 1000 10000 Dos-Reapoens Plot 0.9 0.8 0.7
'. 0.6
- 0.4.
0.3 0.2 0.1
.5 0
9a Organisms obtalnedfromAqfaetlc BloSyst ems. Inc.
05-06--04
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/6/2004 Test ID:
PpKCICR Sample ID:
REF-Ref Toxicant End Date:
5/13/2004 Lab ID:
ITS-Envir. Testing Sol.
Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
PP-Pimephales pmrmlas Conments:
Cone-mg/L 1
2 3
4 D-Control 0.8340 0.8290 0.7770 0.8890 300 0.79S0 0.8400 O.S990 0.7580 450 0.8450 0.8170 0.7590 0.7680 600 0.7140 0.6800 0.6630 0.7250 750 0.3110 0.5320 0.4430 0.2630 900 0.0000 0.1070 0.1620 0.1770 Transform: Untransformed Conc-mg/L,
Mean N-Mlean Mean Alin leRX CV%
N D-Control 0.8323 1.0000 0.8323 0.7770 0.8890 5.500 4
300 0.7488 0.8997 0.7488 0.5990 0.8400 14.063 4
450 0.7973 0.9579 0.7973 0.7590 0.8450 5.115 4
- 600 0.6955 0.8357 0.6955 0.6630 0.7250 4.158 4
750 0.3873 0.4653 0.3873 0.2630 0.5320 31.736 4
900 0.1115 0.1340 0.1115 0.0000 0.1770 71.922 4
I-T:
t-Stat CrH 1.886 0.791 3.089 ailed Isotonic tieal ATSD Mfean N-Mean 0.8323 1.0000 2.290 0.1014 0.7730 0.9288 2.290 0.1014 0.7730 0.9288 2.290 0.1014 0.6955 0.8357 0.3873 0.4653 0.1115 0.1340 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93554729 0.844
-1.0559471 2.44966567 Bartlett's Test indicates equal variances (p - 0.16) 5.24058199 11.3449668 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChY TU MISDu AISDp AISB MISE F-Prob df Dunnett's Test 450 600 519.615242 0.10139043 0.12182689 0.01414623 0.0039206 0.04582928 3, 12 Treatments vs D-Control Organfsms obtainedfrom Aquatic BioSysteems, Inc 05-06-04
Environmental Testing Solutions, Inc.
Statistical Analyses LhtarIntetpolatitn (200 Rtessnolts)
Point
/L SD 95% CL(RTp)
Skew ICOS' 210.70 145.29 35.59 680.44 OA028 JC10 496.40 118.24 26.17 625.81
-1.1384 IC15 576.94 59.09 381.96 634.42
-3.1734 1.0 IC20 614.45 13.35 566.22 646.83
-1.4294 0.9 MC25 634.70 11.40 606.67 672.97 0.5436 0
IC40 695.45 19.33 652.43 773.70 0.7127 0.8 KC50 735.95 21.94 679.54 808.16 0.2319 07/
- udicates IC estanute km than the bowast concentrtkm
- 0.6 00.5 0.3 0.2 0.1 0.0 0
200 400 o00 800 1000 Dose mrgiL Dose.-Repeonse Plot 1-1 0.9.
0.8 i..... 0.05 level 0.7 Islgnhtleane Organisams obtainedfrom Aquatic Bio~ysfems. Inc.
0-60 S06-0"4
Environmental Testing Solutions, Inc.
Page 4 of 5 EnvionmntalTesing oluions In.
Pae 4of Species: Pitnephalespromnelas PpKCICR Test Number:.3Z Daily Clemnistry:
- 0 Dv
- 2l
'Analyst 6
v t.
V\\ Y4 Concentratio Parameter n
CONTROL pH (S.U.)
1->
I*.6X4
--I. Io E. C Conductivity (pmhios/cm)
________0.2 9
Alkalinity (mg CaCOL)
(A \\
\\
Hardness 01-1 (mg CaCO 3/L)
R2 Temperature Z5-3 5
- 2S.0 I
25
( 0C )
pH (S.U.)
7.1.7Z.
T.9 8.0 1.-
to 2
_____7t DO (mg/L) j7.9
- 1.
1.L
- 1-5
?4-300 mg KCIIL Conductivity (limhos/cm) miii Temperature 2l ZS 7 ZS 1
- 24. Z (0c )
pH (S.U.)
1.1Z 3(ao
.; 12.
1.12-(o2 DO (mg/)
8.0 Sr 0Z
- 1.
D
'9.1 450 mg KCV.L Conductivity oI
(~imhos/cm)
Moo___
~
IZ Temperature 25-2Y z.3 ZS.l
.Z pH (S.U.)
1.13 S.01
- 1. (oZ 1.-I -Iz o
DO (mg/L)
.0.Q
.0 600 mg KCilL Conductivity 1310 ! 3 I
Temperature 2
l, 5
ZS,.
5 7q.z pH (S.U.)
7 73
-Q(
E5no
- 7.
1. 3_.1-.5q DO (mg/L)
C>o
_____ &.\\
750 mg KCVL Conductivity
/5B (jimhos/cm)_
tE-;-
Temperature
- 2. L Z
ZS.
- 21. 3
- 25. I Z. 7-
_ _ _ _ _ _ _ ~ ~(-C
)
p1l (S.U.)
-7.i 7 G5 n
T3 0 O DO (mWfL)
.0 e J-S
-. c 900 mg KCUL Conductivity,
, grO W-1, PO
(~mo~M)
________WA0
~
___~
Temperature S3 zq. I 2q.
ZS. i STOCK Conductivity I
i (gjmhos/cm)
-aR Initial (I
Final Initial Final Initial I Final A
.. -.. -- -- - -! :-T- - r-,
.I- - '. '-. -; -I -V :- -
11- .-. I., r..I -.. '.
Environmental Testing Solutions, Inc.
Page 5 of 5 Species: Pitnephtalesproinelas PpKCICR Test Number:_
4 Iy Analyst l
lt_
Concentration Parameter.
5A -4=
l.&t:
59fiX>
4Wt; CONTROL pH (S.U.)
-7.B9 l 3
-3
.19
-9A.
DO (mgtL)
O9 S 1-7.
Ai32 1<;
zO (C~omnu ctivity 3zoo Z
30 3c7 WA (mgCianCtO/L)
\\\\
S Temperature Zq.
3 2'
2 4. >
2 5.D Z4,4 zs.3 4.Z pH (S.U.)
-Jeh2 1.7 -
1 'q n.44 l0
- 1. 3z
- 1. to i
-4.0tol D O (maJ)
( w4
- 17. to 7 q 1-7.
X17.3
,S 30g;I (4m~rhosc/cimi) e)1
)16B2
&i Temperature ZJ6 Z
u-t SD 2*
S2 pH (S.Uj.)
lIq 7
1
~z na-RJ DO (mq/L) 19.2-
- 1. L.
S3. O ll7n.
- 1.
s 3 (3
)8 450 mg lKCVL (Comnd~usctivity20I(Zo IlS ItlO Temperature t3 J&
Z n
Z 5
2.
pH (S.U.)
-1 Lo i
-1. -71q 7-. nz
-1.<3 5' Uq9.
600 mg KCI/L DO (mg/L) 8
.b 1
l 7. (
-7.9 5, f4
-,{E)
R
_Temperature zq 2qJ3 O q.
2t3
- 4.
25 Z,l pH (S.U.)
7t 1 zl8 l n.51%
1.11_
5 X
7(.( 3 5
DO (mplL) 8-.Z Ln q7 d-Xt 8.)01-o7i
+
750 mg KCUL (Conmtdousctivi~ty
)g 4=( 'Fi 1O tIto
- 1.
C D(4mgo/L)
-700 10 q
.Z 0
_ew71 8b 9005-mgKCUL Rodctvt I92
_~
~
Waom0
_Temperature ZY hi 24 4 9
Zl.3 75O Z4j4 S3 Z
DO (rn
)
I~q Final IInitial Final Initial Final Initial Final Initial Final
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 1.20 1.15 1.10 1.05 a
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State and USEPA Mandated Control Limits Limits Set According to + 2 Standard Deviations U
kn M.
U 1.00 0.95 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8
........... "'X
---
.-V.
.I.......................
I I I I I I I I I I I I I I I I I I I I I
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r Laboratory Warning and Control Limits Limits SetAccording to 1O' and 2S5 Percentile CVs
_... _...I..
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
-Warning Limits (mean IC25 :h SA.10)
.Control Limits (mean IC25 + SA.25 or 2 Standard Deviations)
XvLe !
-% --" -.1 11?- I -
-q
'..'?;rs5; Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toidcant Control Chart for Cerlodaphlnia dubia using Moderately Hard Synthetic Water Test nunber Test date 7-day IC21 CT (g/LNaC1)
(g/LNaCI)
State and USEPA S
Centrol Llnits Sp.,
.CT-2S CT +2S Laboratary Warning Llnits SI.S CT -SA.I CT + SAJ Laboratory Contal Ltnits CTr-SA..S CT+SUS USEPA
3 4
5 6
7 8
9 10 I11 12 13 14 is 16 17 is 19 20 12-06-02 12-11-02 12-1lS02 01.07.03 02-04-03 03-05-03 04-08-03 05-06-03 06-04-03 017-0-03 08-05.03 09-10-03 10-01-03 11-0403 12-0403 01-06.04 02-03-04 03-03-04 04-06-04 05-04404 1.03 1.04 1.04 0.96 0.99 1.05 1.03 1.05 1.07 1.03 1.04 1.05 1.02 1.08 1.08 1.06 1.06 1.07 1.07 1.07 1.03 0.00 1.03 1.04 0.08 0.95 1.04 0.01 1.02 1.05 0.08 0.95 1.02 0.04 0.94 1.09 0.08 0.94 1.01 0.03 0.94 1.08 0.08 0.93 1.02 0.04 0.95 1.09 0.08 0.94 1.02 0.03 0.96 1.08 0.08 0.94 1.02 0.03 0.96 1.09 0.08 0.94 1.03 0.03 0.96 1.09 0.08 0.95 1.03 0.03 0.97 1.09 0.08 0.95 1.03 0.03 0.97 1.09 0.08 0.95 1.03 0.03.
0.97 1.09 0.08 0.95 1.03 0.03 0.97 1.09 0.08 0.95 1.03 0.03 0.97 1.09 0.08 0.95 1.04 0.03 0.971 1.10 0.08 0.95 1.04 0.03 0.98 1.10 0.08 0.95 1.04 0.03 0.98 1.10 0.08 0.96 1.04 0.03 0.98 1.10 0.08 0.96 1.04 0.03 0.98 1.10 0.08 0.96 1.04 0.03 0.98 1.10 0.08 0.96 1.12 1.12 1.10 1.09 1.10 1.10 1.10 1.11 1.11 1.11 1.11 1.11 1.12 1.12 1.12 1.12 1.12 1.13 1.13 0.18 0.18 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.17 0.18 0.18 0.18 0.18 0.18 0.1I 0.18 0.18 0.18 0.86 0.86 0.84 0.S4 0.S5 0.85 0.85 0.85 0.S5 0.85 0.86 0.85 0.86 0.86 0.86 0.86 0.86 0.87 0.87 1.21 1.21 1.19 1.18 1;19 1.19 1.20 1.20 1.20 1.20 1.21 1.20 1.21 1.21 1.21 1.22 1.22 1.22 1.22 0.46 0.57 07 0.57 0.46 0.56 0.46 0.56 0.46 0.56 0.46 0.56 0.46 0.56 0.46 0.57 0.46 0.57 0.46 0.57 0.46 0.57 0.46 0.57 0.47 0.57 0.47 0.57 0.47 0.57 0.47 0.57 OA7 0.57 0.47 0.57 0.47 0.57 1.50 1.50 1.48 1.47 1.48 1.48 1.4S 1.49 1.49 1.49 1.49 IA9 1.50 1.50 1.50 1.51 1.51 1.51 1.51 0.64 0.39 0.64 0.39 0.63 0.39 0.63 0.3S 0.63 0.39 0.63 0.39 0.63 0.39 0.64 0.39 0.64 0.39 0.64 039 0.64 0.39 0.64 0.39 0.64 0.39 0.64 0.39 0.64 0.39 0.64 0.39 0.65 0.40 0.65 OAO 0.65 OAO 1.67 0.00 1.68 0.01 1.65 0.04 1.64 0.03 1.65 0.03 1.65 0.03 1.66 0.03 1.67 0.03 1.67 0,03 1.67 0.03 1.67 0.03 1.67 0.03 1.67 0.03 1.68 0.03 1.68 0.03 1.68 0.03 1.69 0.03 1.69 0.03 1.69 0.03 Note:
7-d IC,, day 25% inlnbition concentration. An estirnation of the concentration of odiumn chloride that %ould cause a 25% reduction in Cerodaphnid reproduction for the test poplation.
CT - Central tendency (mean IC,,).
S - Standard deviation of the IC2, values.
Taboratory Contrnl and W2Trdng Units laboratory control and warning liUnits wcre istablisd using the standard deviation orthe IC,, vaues cornresinding to the loth and 25th percentilo CV TIhes ranges are nrc stringent than the control and Warning limits recounmended by USEPA for the test et1;od and cdpoint SX9 - Standard deviation conv~ondinrg to the l0'prcmcnblc CV. (SAc, - 0.08)
S_2 - Standard deviation conveonding to tlhe 25*'percentile CV. (S 1,2 - 0.17)
USEPA Central and WarnTIng Ltnits S rs - Standard deviation correponding to thoe 75b percentble CV. (SAI, - 0.45)
SA,, - Standard deviation cormesponding to the 90* percentile CV. (Skot - 0.62)
CV - Coefficient of variation of the IC3, values.
USEPA. 2000. Undertanding end AccoSting forMethod Variablity in Wlole ElMuent ToxicityApplications Under the National Pollutant DischarE 2l3minSon PegrmnL EPA-833-R-00-003. US ivirnnta1 Ptotection Agency. Cincinrti, OIL 590"44
1.8 1.6 (U
z U
1.4 1.2 1.0 0.8 0.6 Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphlnia dulbia using Moderately Hard Synthetic Water
I 0.4 0.2 O"
-. 0
%A
- 1-51" 4-'3" 0V 0-'4° 0ZTtAO k 0dat'e 0
.A° Z&
h 04 k
Test date
.7-day IC = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Laboratory Warning Limits (mean IC25 t SA10, SA10 = 0.08)
Laboratory Control Limits (mean IC25
USEPA Warning Limits (mean IC2, SA.75, SA75 = 0.45)
USEPA Control Limits (mean IC25 :t SA9 SA 9 = 0.62)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Tes Test date Control Control Mean CT CV CT NISD PM1SD CT number Survival Reproduction for Control Nlean for Control (0)
(offsprinemale)
Reproduction (3/4)
Reproduction
(
fo) ForPISD(3/4)
(ofEipring/female)
CV ('A) 1 12-06-02 100 29.7 11.0 3.0 10.0 2
12-11-02 100 33.8 31.8 13.7 12.3 2.9 8.5 9.2 3
12-18-02 100 30.5 31.3 7.4 10.7 2.9 9.4 9.3 4
01-07-03 100 33.2 31.8 7.0 9.7 2.9 8.6 9.1 5
02-04-03 100 32.3 31.9 8.1 9.4 2.7 8.4 9.0 6
03405-03 100 28.7 31.4 5.1 8.7 3.5 12.1 9.5 7
04-11-03 100 26.3 30.6 6.2 8.3 2.5 9.6 9.5 8
0506-03 100 27.6 30.3 10.8 8.6 3.2 11.5 9.7 9
06404-03 100 25.9 29.8 5.9 8.3 2.6 10.1 9.8 10 07-08-03 100 29.0 29.7 11.6 8.7 3.2 10.9 9.9 11 08-0S403 100 33.3 30.0 6.6 8.5 4.7 14.1 10.3 12 09-10-03 100 29.3 30.0 4.3 8.1 3.1 10.7 10.3 13 10-07-03 100 33.4 30.2 8.0 8.1 3.2 9.5 10.3 14 11-04-03 100 31.0 30.3 7.3 8.1 2.6 8.3 10.1 15 12-04-03 100 30.4 30.3 9.7 8.2 3.6 11.8 10.2 16 01-06-04 100 30.6 30.3 4.4 7.9 3.5 11.5 10.3 17 02-03.04 100 30.7 30.3 7.2 7.9 2.7 8.8 10.2 18 03-03-04 100 29.0 30.3 7.8 7.9 3.0 10.3 10.2 19 04-06-04 100 28.1 30.1 6.2 7.8 2.8 10.0 10.2 20 05-04-04 100 32.5 30.3 6.4 7.7 3.3 10.1 10.2 Note:
CV - Coefficient ofvariation for control reproduction.
On average, the CV for control reproduction is 7.7% in Enmironrmectal Testing Solutions, Inc. Cerfodaphnia chronic toxicity tests.
Lower CV bound determincdbyUSEPA(10*' percentile) -
.9%.
Upper CV bound determined byUSEPA (90* percentile) - 42%
MSD - Miinimum Significant Difference PrSSD - Percent Minimum Significant Difference PhSSD is a measure oftestprecision. The PMSD is the rninimumpercent difference between the control and treatment that can be declared statistically significnt in a whol effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodap.'nia reproduction by 10.2%
from the control.
LowerP.MSSD bound dtemrninedbyUSEPA (10a percentile) - 11%.
Upper PMfSD bound determined by USEPA (90" percentile) - 37 %.
CT - Central Tendancy (NMean Control Reproduction, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted fom 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole EfuentToxicityApplicationsUnder the National PollutantDischrge Elirniation Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati. OIL 05.04-04
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests 40 35 Cj 10
- 1~
U la
,r
%.1a 30 25 I
I Minimum Acceptance Criteria (15.0 offspring per surviving female)
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'-S 5-C C
.a
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Cj U
L..
C2 30 20 10 I
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I North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
In I.I-Test date Control Reproduction or Coefficient of Variation (CV)
Central Tendency (mean Control Reproduction or CV)
Control Limits (mean Control Reproduction or CV 4 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water so 45 40 [
35 0-%
a0`1<
9z Cn P-4 30 25 20 15 E 10 5
0 Test date PSISD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean PMSD)
Lower and Upper PM1SD Bounds Lower PMSD Bound (10t percentile) = I I%, Upper PMSD Bound (9Oe percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Environmental Testing Solutions, Inc.
Page I of 6 Environmental Testing Solutions, Inc.
Page 1 of 6 Jim
- 9c.-c-V.
3-
- h Sum net
- .. ~
0..
200VO&
19 1044 0 d44 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia CdNaCLCR #:
22 Dilution preparation information:
Comments:
NaCI CHIM number.
(MA CA(
H__bn Stock preparation:
100 g NaCIII (dissolve 50 g NaCI in 500 ml deionized water)
Dilutiou prep (mgIL) 600 S00 1000 1200 1400 Stock volume (mL) 9 12 i s5 i
1 2!
Dilunt volum ImL)148S 1485
.1482 1479 Toa1 volume (500 1500 1500 1500 Test organism information:
Test information:
Organism age:
< 24-hours old Randomizing template:
aLue-Date and times organisms i-Ce-Q'-
1LIZ2 -M I Incubator number and 21 1 were born between:
shelf location:
Organism source:
o04.Z1-Om P -r YCT batch:
ABS o'4-71i.d Transfer bowl information:
pH ='I-I SU Temperature -2q.1IC Selenastrum batch: ABS ctlZ1 -
Daily renewal information:
Day Date Test Initiation, Control water batch Analyst renewal, or used termination time 0
0 0-0 O
4 q 1355
__(_-__-O_
4(
I jaltlt 0
2 i e ;_ _A 2
.t -3 oCosA Zo__
os-os-CA 4
O5.O Qkt l04 O'S 5 CS.
O S
os-0 A-5 A a5A3
______O__-
6 os-sc.o'l 1350_______
rE 7
OS II01 loo qtz Control information:
Acceptancec riteria Summary of test endpoints:
% of Male Adults:
OI 7O s 20%
7-day LCSO j71qLn
% Adults having 3' Broods:
JC37 Z 80%
NOEC l
% Mortality:
O7.
s20%
LOEC XX)
Mean Offspring/Female:
- 32.5 i
15.0 offspring/femace ChV ItC8,5A
% CV:
jLaD. 4I
<40.0%
IC25 103ZtA I
3-i' :
I.
e TPage 2 of 6 V.
V Species: Ceriodaphnia dubia Cd-NaCLCR #:
21-CONTROL Survival and Reproduction Data
-Replicate number Day I_l 2
3 4
5 6
7 8
9 10 I
Young produced Q
0 C) 0 0
Or Adult mortality L-
.I-
,T.__
2 Young produced C) I _) O Adult mortality
\\--
L.
.L L
L_
i.....
3 Young produced o ti CY Q-Adult mortality L
L --lL>1 4
Young produced 11 3 fl
3L A
S Adult mortality L-L.
L-s Young produced 1-L2-i IA 1 33 I
12-Adult mortality 6
l Young produced lC:)
C2 Cz-*
l C2 l6 5 5 to l
Adult mortality i
L Lljl L.
7 Youngproduced I ) i llo i
I L 1 I 1 C)
Totalyoung produced l 3 l 34 l
3Z 33 Final Adult Mortality j
l L
1 1C
\\
l Ll.
X for 3' Broods l
(
l l
5B l
J l
Concentration:
% Mortality:
l C)%
Mean Offspring/Female:
- 32. S 600 mg NaCI/L Survival and Reproduction Data I I _____Replicate number Dav 1
2 3
4 5
6 7
8 9
110 I
Young produced 77 C
l z5 D§ y§O
,5l
)W Adultmortality 7
Ir
-Lt>
r 2
Young produced l*.
.ll.ll.l....Q..
Adult mortality l
3 Young produced l
i l
lll Adultmortality I
{
L I
L_
I 4
4 Young produced lI3LL l
(o 1 A
l o
'41 5
I 3 I Adult mortality
'-1 k r
I L
' I Adult mortality 1
T Adult mortality g
L L
l 1
7 Young produced I
1 l1 I
_ I1 I I I
I Totslyoung produed l Z lYc 31 l35
-31 l
31 3-l 33 Final Adult MortalitV L
I l
l t1
-A Spor 69oab I Concentration:
I
% Mortality:
I 07 Mean Offsprine/Female:
I 33iZ_
I %Reduction from Control. - 2.2 l
Page 3 of 6 Species: Ceriodaphnia dubia 800 mg NaCIfL CdNaCLCR t:
22-Survival and Reproduction Data l
-Replicate number Day 2
3 4
S 6
7 l
9 10 I
Young produced l C)
Q C)
C:
)
O C)
C
_O Adult mortal-ty 1l 2
Young produced O C
)
O Q
Adult mortality l
J U
U U
L l_
3 Young produced C) 0 7
_Adult mortality 1lL LLL 4
Young produced LA LA
_4 5
Adult mortality L
l L-l s
Yon prdue 12-I LA
~
1 2 1
-,L7 Z2
-^2 Adult mortality L
U L-l l
L L-...
6 Young produced l C)
T O4O Ic (
0 I5 e
I Adult mortality l
L\\--
L
\\ I-l-L-
l I,,
I Young produced 1 -1 Fl ln 1
IT l! z 1
T
-1 2
l3l-.
ToYloung prduced d
edl l
II l1 Final Adult Mortality I-U.
U I
- 'I I
4 CfAR46. cIC'J i Concentration:
I
% Mortality:
C7.
l Mean Offspring/Female:
l 3.R a
%%ReductionfromControl: -o.'7 0 l 1000 m2 NaCI/L Survival and Reproduction Data Replicate number Day l
1 2
3 4
l 5
6 7
9 I
10 IAdultLt ltalit 2
Young produced j
Q j
0 i
i i
iOj Adult mortality l
l L
l 3
Young produced l l
J l
C) l O
Adult mortality l
U lL.
l I llUl L
l ti 4
Young produced LI LJ j
(
j 3
j j
j j
a a
j ti Adult mortality l
i l
L I L
1
- I l
Youngproduced 12 j 1
3 l
j 3 l3 j I l I LA i 1 I
iA Adult mortality a
a L
a l
6 Young produced i o i
i Adult mortality l
jUJ U
6 Young producedLo i
F1T hO 1
Totalyoung produced lo 31 32 21 1231 321 L
l 2 Tq Fina1 Adult Mortality l
U l
Q l
"EC t i t?
C L
7-.
-Y-CAPM Ojee-Concentration:
% Mortality:
l1 Mean Offspring/Female:
l 31. S
% Reduction from Control:
3.17,,1
.1 t*
Environmental Tcsting Solutions, Inc.
Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:
2-2 I
I I
I I
)
U I
I i
I 1200 m2 NaCI/L Survival and Reproduction Data Replicate number Day 1
f 2
3 4
5 6
7 8
9 I
10 I
Youngproduced Q
o cI I o L Adult mortality
-t-1 2
Young produced O
O O
Adult mortality
_- L LI L-l 3
Young produced r) l O
o 1
)
0.
O Al
- o.
O Adult mortality i,
l L-l L-LL 4
Youngproduced O
I o
_3 131 33Z 2Z Adult mortality L
l 1 r_
5 Young produced I
l l- (*
1 1 -3 t4
-3 Adult mortality l
l l
l l
l l
l l
6 Young produced l
(p l
j Adult mortality l l
1 l
7 T Young produced r I
T o
3 1 o l
7 I
I Totalyoungproduced I q
(
r 9 1,o l 1 5 l 9 Final Adult Mortality U
T T
l l
l l Concentration:
% Mortality:
l Mean Offspring/Female:
I1.9 l
% Reduction from Control:
k;5t.41 1400 m NaCl/L Survival and Reproduction Data I_____
IReplfcate number I
Day l
Il 2
3 4
5 6
7 8
9 10 I
Young produced Q I C
C) 0 G
C) rJ l
Adult mortality
( l
[
l L
l L
T 7
l 2
Youngproduced l
Ql ) l I
Adult mortality
\\
l L1 3
Young produced l
I Adult mortalilty L+
'-- + !----
lAdult-mortallty-L l
l Young produced j Adult mortality l
l l
lL l
L-rL L
l Y
Young produced lI3 O j
C) l I
~~Young produced [ 0lIl5_l 1
3 s-SL
-n-X 3 l laAdult mortality l~
l 1> l l
I all Total young produced 21 1
ID A
c 1101 FFinl 2Adult MNortaflty U.
L.- T*-=.....7*-
-. LL" Concentration:
% Mortality:
i ic -I Mean Offspring/Fernale:
1,.
% Reduction from Control: l
.0 1
Environmental Testing Solutions, Inc.
Verification of Ceriodaphnia Reproduction Totals Control DRe licate number Day I
2 l 3 l 4
5 6 l 7
8 91 10 Tota I
0 o 0 0o 0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
3 4
4 4
3 6
4 6
4 5
43 5
13 13 11 14 12 13 14 13 13 12 128 6
5 0
0 0
0 0
0 15 15 16 51 7
10 17 16 16 13 14 17 0
0 0
103 Total 31 34 31 34 2S 33 35 34 32 33 325 600 mg NaCI/L eRc licate number Total Day 234 5
6 7
9 10 0
O O
O O
O O
O O
O 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 3
6 4
4
.6 6
6 4
5 3
47 5
12 14 12 14 1 1 15 13 13 1114 129 6
0 0
0 0
0 0
17 0
16 16 49 7
13 16 15 17 14 18 0
14 0
0 107 Total
- 28.
36 31 35 31 39 36 31 32 33 332 800 mg NaCI/L Day lepicut nuberTotal D2y l I 3
4 5
6 l 7 8
9 10 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
__ o 3
0 0
0 0
0 0
0 0
0 0
0 4
4 6
5 5
4 3
5 4
6 4
46 5
12 13 13 14 13 1 1 12 14 12 12 126 6'
0 0
0 16 0
1 0
0 1
SX 7
14 15 15 0
17 1
13 Total 30
_34 33 3
34 30 31 1000 mg NaCI/L I Da I RepIlcate number Total D Y I1 2 1 3 4 t S 1 6 1 7-1%
9 1
T0ota 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 o0o o0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
6 3
3 4
4 4
4 5
5 42 5
1 2 1 5 1 0 13 3
1 1 14 1 0 1 0 14 122 6
0 16 1
0 0
0 0
0 0
15 32 7
1 4 0
18 13 14 17 61314 0
119 Total 30
.37 32 29 31 32 34 27 29 34 315 1200 mg NaCIIL l
Day l lReplcate number Total I
0 0
0 0
0 0
I O0 O0 0
2 o
o o 0 o
o o 0 0
3 03 0
0 o 0
0 0
0 0
0 0
4 0
0 2
3 0
3 3
2 2
0 15 5
1 6
4 7
3 6
2 3
4 3
39 6
6 0
0 7
0 0
0 01 0 0
13 7
7 10 3
0 7
9 10 4
0 2 1 52 Lotal 14
.16 9
17
_10 1 IN 15 9
6 1
119 1400 mg NaCI/L Day cplc.e number Total 1
2 l 3 l 4 5
6 C 7
8 9 l 10 I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0o 0 O o
0 0
4 1
2 0
2 2
2 1
1 I
0 12 S
0 3
3 1
4 3
4 2
6 0 1 26 6
0 0
0 0
0 0
0 4
0 0
4 7
1 6
1 5
0 3
5 1
3 0
25
'Total l 2 11 4
8 6 l 10 10 0
67
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Cerfodaplnia dubla Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number.
CdNaCICR P51 (#22 at 351 Depot St.)
Test dates:
May 4-11. 2004 Rlevelved by:
C Concentration
=
_eriIate number Survival Average reproduction c.fekf.irkt o
rn, dca fbm (mfL NaCI) 1 2
3 4
5 6
7 S
(%)
(offsprlng/female) artden. (%)
t"abrt (%)
Control 31 34 31 34 2S 33 35 34 32 33 100 32.5 6.4 Not applIcable 600 28 36 31 35 31 39 36 31 32 33 100 33.2 9.S
-2.2 800 30 34 33 35 34 30 34 31 33 34 00 32.J 5.5
-0.9 1000 30 37 32 29 31 32 34 27 29 34 100 31.5 9.4 3.1 1200 14 16 9
17 to 18 15 9
6 5
100 11.9 39.3 63.4 1400 2
II 4
8 6
S 10 8
10 0
90 6.7 54.5 79.4 Dunnett s iMtSD value:
PMSD):
3.291 10.1 MSD -
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure oftest precision. The PMSD is the minimum percent diffeence between the control and treatment that can be deelred statistically significantin a whole emuent toxicitytest. On aveage a significantdifference occurs forEnvironmentalTestingSolutions Inc. chronictoxicitytests when a toxicant reduces Crifodaphnfa reproduction by 10.2% from the controL Lower PMSD bound determined byUSEPA (I0' percentile) - I I%.
Upper PMSD bound determined by USEPA (90*' percentile) - 37X.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories ror CeriodapAnla reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting rot Method Variability In Whole Emuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-S33-R4O0-003. US Environmental Protection Agency. Cincinnati. 011.
Environmental Testing Solutions, Inc.
Statistical Analyscs Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date:
5/4(2004 Test ID:
CdNaC]CR Sample ID:
REF-Ref Toxicant End Date:
5/11/2004 Lab 1a ETS-Envir. Testing Sol.
Sample Type:
KCL-Potassium chloride Sample Datc:
Protocol:
CHRONIC-EPA-821-R-02-013 Test Species:
CD-Ceriodaphnia dubia Cormnents:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10 D-Control 31.000 34.000 31.000 34.000 28.000 33.000 35.000 34.000 32.000 33.000 600 28.000 36.000 31.000 35.000 31.000 39.000 36.000 31.000 32.000 33.000 800 30.000 34.000 33.000 35.000 34.000 30.000 34.000 31.000 33.000 34.000 1000 30.000 37.000 32.000 29.000 31.000 32.000 34.000 27.000 29.000 34.000 1200 14.000 16.000 9.000 17.000 10.000 18.000 15.000 9.000 6.000 5.000 1400 2.000 11.000 4.000 8.000 6.000 8.000 10.000 8.000 10.000 0.000 Transform: Untransformed I-Tailed Isotonic Conc-mVIL Mean N-Mean Mean Alin Max CV%
N t-Stat Critical MSD Mean N-Mean D-Contrml 32.500 1.0000 32.500 28.000 35.000 6.364 10 32.850 1.0000 600 33.200 1.0215 33.200 28.000 39.000 9.817 10
-0.486 2.287 3.291 32.850 1.0000 800 32.800 1.0092 32.800 30.000 35.000 5.529 10
-0.208 2.287 3.291 32.800 0.9985 1000 31.500 0.9692 31.500 27.000 37.000 9.376 10 0.695 2.287 3.291 31.500 0.9589 01200 11.900 0.3662 11.900 5.000 18.000 39.306 10 14.314 2.287 3.291 11.900 0.3623
- 1400 6.700 0.2062 6.700 0.000 11.000 54.522 10 17.927 2.287 3.291 6.700 0.2040 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorv D rest indicates normal distribution (p > 0.01) 0.79752886 1.035
-0.2104094
-0.3540704 Bartlett's Test indicates equal variances (p - 0.07) 10.1435528 15.0862722 Ilypothesis Test (1-tall, 0.05)
NOEC LOEC ChV TU MISDu MSDp MSB MSE F-Prob dr Dunnett's Test 1000 1200 1095.44512 3.29082322 0.1012561 1465.50667 10.3555556 9.4E-30 5, 54 Treatments vs D-Contml 05-04-04
Environmental Testing Solutions, Inc.
Statistical Analyses LMnMr detipolbffon (O2o Rensunpls)
Skew
?Ibsi1 SD 9S% CL IC05
'CdO iCis 1C20 M2S IC40 IC50 1002.98469 1019.7449 1036.5051 1053.26531 1070.02551 1120.30612 1153.82653 47.1935868 9.34608206 7.83352583 7.73480096 7.79416206 9.10946274 866.356731 1001.68826 1019.S6166 1037.9063 1054.95321 1103.66182 1016.02148 1032.79825 1049.53428 1067.17241 1036.16426 1140.86506 1178.32457
-1.2938
.1.750
-0.2348
-0.1677
-0.0814 0.1764 0.2784 10.6783798 1134.3475 1.0 0.9 0.8 0.7 0.6 0
a1 W 0.3 0.2 0.1 0.0 40.1 0
500 1000 Dose mg/L 1500 Dote-Response Plot talt. 0.05 level of slgnleance o0 0
0 0
40 10 0
0 0
a 0T 05.04-04
Environmental Testing Solutions, Inc.
Page 5 of 6 Eniomna etn oltos n.Pg-r Species: Ceriodapltnia dubia CdNaCLCR #:
2.Z Daily Chemistry:
~Dayv
_Analyst MAi>
cm C
I
(
Concentration Parameter
__Q_
pH (S.U.)
5.(rl 12 1.3 17-7i
-7t, 13
-7.15 DO (mgL)
-i 1q
-4
-7 R 1..
8 CONTROL Conductivity
(. (mhos/cm) 2> 1.30031 Alkalinity (mg CaCO 3/L)
&o_____
Hardness (mg CaC OVL Temperature
(°C) 21J.2 ZS__
__Z.3 Zl__S O
t pH (S.U.)
15
.4 7.10 1-Lug 1 -. q 7.loW DO(metL) 7
.n 8.1 Bc 600 mg NaClVL Conductivity Gim hos/cm )
1 1 7 0 Temperature pH (S.U.1 Slf)f5lt 76 o
ea 7.(,I-DO (m_
_/L)
(0C) 2 2.
O U 0 S
1000 mg NaCiIL Conductivity (gmhos/cm) 1_7__0
- -5 q
Temperature I
2S.3 ZA j __
_ (°C)
S 3
2 Z
l5Z
.t 21 pH (S.U.)
1 7(
7 -i 1.t3 7.t21 7.(A I
DO (mg/L) la4
-.O 8_ 3
_1._
1
-S o.
1000 mg NaClL Conductivity (umhos/cm)
ZZ..&IQO
- I
_ _1 Temperature ZI.Z.L 2S 3 zi
( I C q'A2 3
Z43_
pH(S.U.) l
- 7. to-
-7n I5
-7.
7
- 7. 1C DO (mg/L) 4
__.o 0.3 c
8,Y 1200 mg NaCilL Conductivity (pimhos/cM')
2 qetV0___
Temperature
-ZqIio 2 S(
VIA-I (PC)
A 3
25.3 pSC (S.U.)
1-7 5.
5 7.te3
'740 7
-7.15 DO (mg/L)
'.t B.0 l
S-:
-1.7 8.o 9 n 140D mg NaCU/L Conductivity z
)C w
Z3i i
s5 Temperature 2q.1 7
zs3 2.513 Zq. to ZS. I q
26-L STO~~~i Conductis ity Zot Initial Final I Initial 11 Final Initial Final
.nta I
Fnl nta i a
'Environmental Testing Solutions, Inc.
Species: Ceriodaphnia dubia A
y Analyst Page 6 of 6 CdNaCLCR :
2-Day 4
S 6
,. -,
9 9
w -
Y -
A\\\\
ll
- K4 14qea 1I (rM w
- e.
- r-
'Concentration Parameter pH (S.U.)
8-oz.
-I-.E)I
-. I',;
I-Uq CONTROL DO (mg/L)
GO l
- b.
8 a
Conductivity Alkalinity (mg CaCO 3/L) \\
Hardness (mg CaCO3/L)
Temperature (0C) 24.1
%5.0
-s5-I
.600 mg NaCitL
'64 pH (S.U.)
Th~ot h.12 DO (mg/L) 8.1 l
R.
8 2
Conductivity I
° I
(pimhos/cm)
. NQqo0
~
1 I40~ 11 143o Temperature'.
(0C) z5.z-24.L, 2A:9 zq. 6 z4q-c Zs...
- 24. So pH (S.U.)
I
-4n.S
- n.
7
.(09
-7.51
-7 *151
. t# Z DO (mgfL) 6*1 e
£.
\\
¶.Z.
8.2.
800 mg NaCVL Conductivity 11 Z
O 1
Temperature 725-I 2ZL\\.
211.a zq Z
-js.Z
-zq9 pH (S._U.)
-_I I
((
-1).-I 2.-
F sq DO (mg/L) 6-Z Z___.
t R8 6.j BL Z-1000 mg NaCVL Conductivity.
(Jimhos/cm)
.Zo-o IC 23Z 2510 ISO__
Z___
Temperature (0c)
ZS 2 2Y.7.
0 a
zso z
l;.
p(S.U.)
7.(
1.6-1 7.R G
.9 jr<r 1
-°7.!5 tq DO (mg/L)
- a.
1-S a >
1200 mg NaCVL Conductivity 2
° (pmhospcm) 2S.q
__ 232. l0 l
Temperature
~
- 2.
S.I z,
z pH (0C U)
L DO mig/L) 8S.-
9 S-LI 1400 mg NaCIIL Conductivity Orlocm) 217
-2
- DjD.9OI Temperature 2TS2.
2q (.
I 24.&
21-
- 25. Z 21A (0c )_
STOCK Conductivity (umrhos/cm)
IMN',
lZ~wo>
a l
Initial Final Initial h Final I Initial Final I Initial Final I
I
PERMITTEE NAMEIADDRESS (Include Facility NameAtocation if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT -
Address P.O. BOX 2000 ____________
__ _ _11N ROM9CE S
-2B -
S__ODDY-DAISY_
TN 37384_
Fac dy__WA-SEOUOYAHNUCL EARRPLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form DISCHARGE MONITORING REPORT (DMR)
ForrnB (SUBR 01) 0Ml3b TN026450 l103 G
F - FINAL PERMIT NUMBER I DISCHARGE NUMBER LOW VOL WASTE TREATMENT POND I -MONITORING rPERIOD EFFLUENT Approved.
No. 2040-0004 YEAR I MO DAY I lYEAR lMO AY From i 04 1 os 1 01 I To 04 1 oi 1 1
... NO DISCHARGE Z
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE E X O F.
T Y P E AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.0 9.0 12 0
13/31 GRAB MEASUREMENT 00 0.
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___,WE SOLIDS, TOTAL SUSPENDED SAMPLE 134 175 26 16 20 19 0
4/31 GRAB MEASUREMENT 26 00530 1
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SAMPLE MEASUREMENT REQUIREMENT'*
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SAMPLE MEASUREMENT REQUIR EMENT.....
NAME/TiTLE PRINCIPAL EXECUTIVE OFFICER IICertify under penally of law thlat thliS dacunment and all attachments were prepared under my
^t/
TELEPHON E DATE J. R n y D utiredtion or supervisIonIn accordance with a ystem designed 10assure that qualiied personnel r'_ U VJO'rl
- 9. A I J. Ra ndy Douet
~ ~
'ropry gathner and evaluate the Information submitted. Based on my inquiry of thne person or S
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Ipersona who mranage thle system, or those persona directly responsible for gathlering thle PD n i a En i o m n a En in e 42 84 -6 0 04 06 10
~~~information, the inormation submited is, tolthe best of my Irnowledge andt belief, true.
_________________________42_84 -670_04_6_1 Site Vice President exrae. nd complete. I am aware that there are significiant penalties for submiting false SIGNATURE OF PRINCIPAL EXECUTiVE TYPED___
OR__PRINTED formation. Indluding the posaibitity of lne end imprisonment for iknowing violations.
OFFICER OR AUTHORIZED AGENT AREA NU BE YEAR MO DAY T Y P E D O RERI N T E DM E N_
COMMENTS AND EXPLANATION OF ANY VIOLATiONS (Reference all attachments here)
EEed i
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1 o 1 EPA Form 3320-1 (REV 3199)
Previous editions may be used Page 1 of 1
PERMITTEE NAMEJADDRESS (Include FacilityName/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
_ _ _ _LNTEROFFICE SB-LA).
SOOD Y-DAISY_
TN 37384 Fadlity W A SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040-0004 L
TN_06l 107 G5l F - FINAL I
PERMIT NUMBER I [DISCHARGE NUMBER METAL CLEANING WASTE POND I
MONITORING PERIOD I
EFFLUENT D
YEAR MO DAY
- NO DISCHARGE E1 i
From 04 05l01 I To 04 l 05 31 NOTE: Read Instructions before completinq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE X
OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT 12 00400 1
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SAMPLE 19 MEASUREMENT 19 01042 1
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.CAL FLOW, IN CONDUIT OR THRU SAMPLE Oa TREATMENT PLANT MEASUREMENT 03 50050 1
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all uttachrments were prepared under my ITELEPHONE DATE diretbon or supervision In accordance With a system designed to assure that qualified personnel
-U AI 1
r J. Randy Douet property gather and evaluate the information sutminted. Based on my inquiry of the person or CL/t.9j persons wtho manage the system, or those persons directly responsible for gathering the Principal Environmental Engineer 423 843-6700 04 06 10 Inomtothe Information subimitted Is.* to the best of my knowledge and belief, true, 423___843__6700___04__06__
I Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED inforsnation, including the possibility of fine and imprisoment for nowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)
No phosphorous bearing cleaning solutions were used. No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous editions maybe used Page I of I
PERMITTEE NAME/ADDRESS (Include FacilitNameAlocat ionifDifferent)
Name TVA - SEQUOYAH NUCLEAR PLANT ____
Address P.O BX 2000 -
____Q tlN EROFFCE B-2A)__________-
-_-_-_SODDY - DAISY TN 37384 fgfity_ VA - SEQUOYAH NUCLEAR PLANT_
Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
JTNOO26450 110 G I PERMIT NUMBER I DISCHARGE NUMB13E R l
MONITORING,PER1012 MAJOR (SUBR 01)
F-FINAL RECYCLED COOLING WATER EFFLUENT
- -- NO DISCHARGE xxj -
NOTE: Read Instructions before con Form Approved.
OMB No. 2040-0004 IYEAR rIMO DAY YEAR
-MO I DAY From 04 1 05 1 01 ITo 04 05 1 311 T
I*
I
,,I I
NAMElTiTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my II It I
TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel J. Randy Douet properly gather end evaluate the information submitted. Based on my inquiry of the person or
'1k phat O.&
XtV4 persons who manage the system or those persons diredtly responsible forgathering the Principal Environmental Engineer 423 843-6700 04 06 10 information, the informatIon submitted Is, to the best of my knowledge and belief, true,
_____________Egne_43 836000_6 1
Site vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPEDORPRINTEDinformation, Including the possibility of fine and irprsonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY T Y P E D O R PR IN T E D C O D E COMMENTS AND EXPLANATION OF ANY ViOiATiONS (Reference all atachments here)
No Discharge this Period EPA Form 3320-1 (REV 3192)
Previous editions may be used Page 1 of I
PERMITTEE NAMEIADDRESS (Include Facility Name/locaton if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT ___
Address P.O. BOX 2000
_ _LNTEjLROMFCE S1B-ZN-----
SODDOY DAISY TN 37384 Fadci TVA - SEOUOYAH NUCLEAR PLANT Locatron HAMILTON COUNT)L ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MOWITORING REPORT (DMR)
(SUBR01)
I TN026450 l116 F
- FINAL I
PERMIT NUMBER IDISCHARGE NUMBER BACKWASH Form Approved.
OMB No. 2040-0004 RMNmll'nalmrs' DCstnr%
I EFFLUENT I
Im YEAR I 051l 01 ToYEAR MO 04 NO DISCHARGE E
From1 0 1 0
1 1 I rol04 105 31NOTE:
Read Instructions before comnpleting this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certifyunder penalty of law that this document and all attachments were prepared under my I I
TELEPHONE DATE c
iretion or supervision In accordance with a system designed to assure that qualified personnel TELEPH^rONenE Df J. Randy Douet pioerty gather and evaluate the Information submitted. Based on my inquiry of the person or A 't-t L
A persons who manage the system, or those persons directly responsible forgathering te Principal Environmental Engineer 423 843-6700 04 06 10 Site ePresident inforjtion. the Information submitted is. to the best of my knowl4edge and bellef.ue, aue S ite Vice Presidentaccurate, and complete. I am aware that there are sIgnificant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE
_______ ORPRINTEDinformation. rictuding the possibility of fae and Imprisonmentfor knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
f CODEi zl COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments here)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199)
Previous eftons may be used Page 1 of I
PERMITTEE NAMEIADDRESS (Include Facility Name/locatln if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
_[iNjEROFFCE SB-0 SODDY - DAISY TN 37384 adfl iVA - SEOUOYAH NUCLEAR PLANT Locaton _AMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 1)
I TNO026450 117 G I F-FINAL I
PERMIT NUMBER IDISCHARGE NUMBER IBACKWASH I
MONITORING,PERIOD EFFLUENT Form Approved.
OMB No. 2040-0004 From 04 1 OS I 01 To I04 0
31 t**
NO DISCHARGE D
NOTE: Read Instructions before completing this form.
PARAMETER I
4 4
4 4
DEBRIS, FLOATING (SEVERITY) 01345 1
0 0
EFFLUENT GROSS VALUE SAMPLE MEASUREMENT
- . -PERMIT.?- -
. REQUIREMENT,;
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REQUIREMENT.
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I NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this doalrnent and all attachments were prepared under my 1
I TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel I
.f
/t J. Randy Douet mwperty ttather and evaluate the ireormration subzmitted. Based on my Inquiry ofthe person or
)W hV L~0,0,
)JA persons who manage the system, or those persons directly responsible for gathering the in omation f
thIormation submitted is. o the best of my knowledge and berief, true, Pncipal Environmental Engieer 423 843-6700 04 06 10 Site Vice President acurate. and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPED OR PRINTED formation including the possibility of fne and Imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all affachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes.
_PA Form ~O.¶ REV 3I__t)~_:
Previous ecitions may be usesi Page 1 of 1........... _::____..............L_.
EzPA Fonfn 3320-1 (REzV 3/99)
Previous eafrions irlay oe usea Page I of 1
PERMITTEE NAME/ADDRESS (Indude Facility Name/Location iDifferent) ame TVA - SEQUOYAH NUCLEAR PLANT -
Address P.O. BOX 2000 ____________
EEIOFFE 3-LA
SODDY - DAISY _
TN 37384 aciiy_ TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR I
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) l TN026450 l
118LG F -FINAL l
PERMIT NUMBER lISARGE hUMBER WASTEWATER & STORM WATER Form Approved.
OMB No. 2040-0004 I
nMfNITA)PIW(_ PFRIplr 1
EFFLUENT I YEAR MO DAY I YAR MO DAY
^^^ NO DISCHARGE E
From 04 05 I01I To 04 l05 31N NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE E X OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE 19 MEASUREMENT 00300 1
0 0
PERMITi5.
f'.0.
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GRAB-MEASUREMENT 00530 1
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GRABj*P TREAMEN PLAN MESUEMNT.K EFFLUENT GROSS VALUE REQIRM-EN :AVG...
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- E r-SOLIDS, ETOTLALSUPENSAMPLE MEASUREMENT 00545 1
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GRAB-EFFLUENT GROSS VALUE EQEE..
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FOLOWS, INTCONDUT OSAMPLE 0
MEASUREMENT 50050 1
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.s NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this5 documnent and all attachments were prepared under my
-A i
Il
/
TELEPHONE DATE J.RnyDutdirection or supervision inaccordance witha system designed to assure Ihat quaified persiomel vlnw"X/d~lt r_
J. Randy Douet ~properly gather and evaluate the Information submitted. Based on my Inquiry of the person or _vtttt*~4 p.soswhmbaetHeytt e mene rsthosteptersosiretly espnsibtefror gathering the Principal Environmental Engineer
.423 843-6700 04 06 10 Site Vice President accurale, and com~plete.
I am aware that there are significant penalties for submitting false SGAUEOF PRINCIPAL EXECUTIVE_
________________information.
Including the possibility of fine and imprisonment for knowing violations.
FIE OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE_
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA Form 320.1 (REV
- 99)
Previuseitions may beusd ae o7 EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of I