ML041070053
| ML041070053 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 03/09/2004 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| TN0026450 | |
| Download: ML041070053 (116) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 March 9, 2004 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2004 Enclosed is the February 2004 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pned cnrcyc pw
PERMITTEE NAME/ADDRESS (Include FacgitvNameltocation ifDliferent) NATIONAL POLLUTANT DISCHARGE EUMNATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 I TNOO26450 { 101 G I F - FINAL SODDY- DAISY_ TN 37384 _______ L PERMIT NUM3BER I DISCHARGE NUMBERl DIFFUSER DISCHARGE Fa y_ TVA. SEQUOYAH NUCLEARAPLANT EFFLUENT l
I
^ - - ^ ^ z- - - . E .
PFf1~
l Locaton HAMILTON COUNTY I MnNIT1^,r31k 1'1"v"1 '4n11.1 r~n YEAR F Mo I DAY YEAR MO DAY *+/- NO DISCHARGE 0 ^*'
ATTN: Stephanie A. Howard From 04 l 02 l 01 To 04 02 29 NOTE: Read Instructons before completinn this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
________ OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 10.3 04 0 29/29 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 ~1*eaeJ ~ *~~** r~e~*~ O E.C ~g'.E~ CK.REQ
- E~~.
INSTREAM MONITORING J!REfOUIREMENT, .! . . AILY.MXC ________
TEMPERATURE, WATER DEG. SAMPLE 22.9 04 0 29129 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 0 . .. PERMRDEG. C. SEEK' EFFLUENT GROSS VALUE -REAUIREMENVT 1 ° 4 1 29 GRE DAI MXPR PH SAMPLE 7.4 7.8 12 0 8229 GRAB MEASUREMENT 0040 1 0 0 A ,0WEEKLY. GRA EFFLUENT GROSS VALUE 'adzIMMT!!.4 ~ '-~l ~ ~ MINMUM.~ .. ~ MAXIMUM- 0. -
'-.o' '- .-
SOLIDS, TOTAL SUSPENDED SAMPLE *aeee7 10 19 0 4/29 GRAB MEASUREMENT 00530 1 0 0 -eee* '* 30yv MGIL '-~WEEKLY iGRAB, EFFLUENT GROSS VALUE ~ ~MNl ~ ~ ~ ~ . ,--MOG ~~~v'____
OIL AND GREAS E SAMPLE eeaee *eae .a.e<5 <5 0 4/29 GRAB MEASUREMENT
- 5. 6 .1 0 *,PERMIT* WEEKLY. GRAB3 005- PRI'Z 1 *eee- ~ * ~ **~~****,.; f . MGtL EFFLUENT GROSS VALUE EQREEh *1 Z 't A.
FLOW. IN CONDUIT OR THRU TREATMENT PLANT SAMPLE MEASUREMENT 1581 03 o 29 (29 RCORDR 50050 1 0 0 ERMITlij-';RPOT~, MGD ~ ~~.ONTIN'Z RCORDR EFFLUENT GROSS VALUE REQUREMntic kZ'y MOUSLr./s,'~~~~i CHLORINE, TOTAL RESIDUAL SAMPLE 'e.e *** <0.006 0.014 19 29(29 GRAB MEASUREMENT '
50060 1 0 0 -~,I~PERMIT?;,,.~t, i ***e~~ P~e*ae"~' ~ ~ ~ Ch06~.i; .5,7 MG[I. , WEK-,: CALCTD EFFLUENT GROSS VALUE
________________ TEURM~ri;,'!
z".-________ ______ .
_____________DYS . INST MAX,.,,,___ ___
NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this domaent and aWlattachnents were prepared under my -
.1direction or supervision In accordance with a system designed lo assure that qualified personel ' -I r /)U 1cxn co C Richard T. Purcell propery gather and evaluate the Information submitted. Based on my inqluiry of the person or 4Ali-4 04 4 Aktv_
persons who manage the system or those persons directy responsibie for gatherin the Principal Environmental Enineer Worrnation, the Information submitted Is to the best of my knowetdge and belief, true, I Site Vice President accurate, and cornpate. I em aware tVat there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTiVM I Vormationr Including the potsibility of roe and Imprisonment for knowtng violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED I - - - - - - - - ____ ___ -
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference afl attachments here)
No dosed mode operation. The following Injections occured: 1. PCL-222 (max. calc. conc. was 0.027mg/L-limit 0.1OOmglL) 2. CL-363 (max. calc. conc. was O.OOmg/L-iimit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.027mgtL-limit 0.100mg/L)
.,~I . . .1 . . .I Paqe I of 2
PERMITTEE NAME/ADDRESS (tnclude FacivitvName/Locationn if OiferentI NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved.
Name -ASEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000____________
I TN0026450 101 G I F- FINAL
___SODDY. ~_ B2_
_ DAISY TN 37384_
qlT~~c _Lj _ _ _____ _ _ IPERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE FaojwWA- SEOUOYAH NUCLEARPANT___
Location HAMILTON COUNTY __________ I Mnw)MTnir)P~' Priirinr 1 EFFLUENT ATTN: Stephanie A. Howard From Frm 4
04 MO 0 DAY 02 l 01 I To Y2 040 M DAY 29 NO DISCHARGE El ^
NOTE: Read Instructions before completinq this form.
1*
COMMENTS AND EXPLANATION OF ANY VTO CCW data for February 2004 Is attached.
rn P%r.,, ityn i irrmriQ9l PrPvvrt M~illonimtv heus~ed Pg 2 of o 2 Page
CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 2/23/2004 1030 <0.5 mg/L 2/28/2004 0230 CVS EPA 8015B CCW TRENCH DaterTime Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 2/23/2004 1040 <0.5 mg/L 2/28/2004 0308 CVS EPA 8015B
PERMITTEE NAME/ADDRESS (Include Facility NamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR For rn Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT ____ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BO 2006 ____________
I TN002 0 101 T F - FINAL 1RF9C _ _L_q._P E_ _ __ _ _ _ _ _ I PERMIT NUMBER I IDISCHARGE NUMBERI BIOMONITORING FOR OUTFALL 101
.Eac4!b'... WA SEQUOYAH NUCLEAR_PANT_ _ _
Lcaon H AMILTONC UN T__________ I MONITQRING PERIOD tEFFLUENT ATTN: Stephanie A. Howard From Frm
~04 IM 0 fl YI T o 01 I To EAR MO 0 4 l 02 l 29 l DAYI *-- NO DISCHARGE i ...
NOTE: Read Instructions before completinq this form.
I If I i
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify -rider penaly of law that this docunenl and al"attarrnents were prepared under my
- Il TELEPHONE DATE i bY,4_
Richard T. Purcell
. or supervision In acordance with a system designed to assure that qualified personnel
,redion properly gather and evaluate the Information submitted. Based on my Inquiry of the person or persona twomanage the systenI or those persons directly responsible for gathering the A
UbI fafl h v
o Flf v -__
)
Principal Environmental Engineer VcPation, the Information submitted is t the best of my knowledge and belief, true. _________ 423 843-6700 04 03 09 Site Vice Pre ent accate, end complete. I am *warethat there wresignificant penalies for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE T Y PE D OR PR IN TE D information.
incdiuing the possibility of_ ine and Imprisonmrimt for knob*tg violations.
OFFICER OR AUTHORIZED AGENT AREA CO DE NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all atachments here)
Toxicity was sampled from 2112004 to 26/2004. Report Is attached.
EPA Fnrm 33?n-1 (REV 31`99) Previrlitseditions mav he used P nrp I of I
March 4, 2004 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, FEBRUARY, 2004 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected February 1-6, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN February 2004M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: March 4, 2004
- 1. Facility / Discharger: Seguovah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDESPermit#: TN0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. DesignFlow(MGD): 3266
- 7. lQ10: 2,992.4
- 8. Outfall Tested: 101
- 9. Dates Sampled: February 1-6,2004
- 10. Average Flow on Days Sampled (MGD): 1569,1567,1565
- 11. Pertinent Site Conditions: H13OM was not injected from February 1-6, 2004. No unusual conditions reported. Production / operation data will be provided upon request.
- 12. Test Dates: February 3-10. 2004
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphniadubia)
- 15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 UV treated Intake: 100.0
- 16. Permit Limit Endpoint (%): Outfall 101: IC,5 = 43.9%
- 17. Test Results: Outfall 101: Pimephalespromelas:IEd> 100%
Ceriodaphniadubia: E a>
100%
UV treated Outfall 101: Pimephalespromelas: IC? > 100%
- 18. Facility
Contact:
Ruth Ann Hurt Phone#X: (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 350-9364
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101 samples collected February 1-6, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101. Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/Y)/ (MMIDD/YY)/ Arrival Initial (MM/DD[YY)/
Sample Time (EST) Time (EST) Temp. TRC* Time (EST)
ID Collected Received (OC) (mgL) Used By 101 02/01/04 0734 to 02/02/04 1453 1.2t <0.10 02/04/04 1513 02/01/04 07193t 02/03/04 1509 Intake 02/021/04 0619 02/02/04 1453 1.0 <0.10 02/03/04 1509 02/03/04 08471t 02/05/04 1526 101 02/04/04 0747 02/04/04 1400 0.6t <0.10 02/06/04 1502 02/03/04 08047t 02/05/04 15026 Intake 02/04/04 0704 02/04/04 1400 0.8 <0.10 02/06/04 1502 02/07/04 1500 101 02/05/04 0713 to 02/06/04 1320 0.3t <0.10 02/08/04 1509 02/06/04 0613 02/09/04 1502 02/07/04 1500 Intake 02/05/04 0733 to 02/06/04 1320 0.1 <0.10 02/08/04 1509 02/06/04 0633 02/09/04 1502
- TRC = Total Residual Chlorine t Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.0C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart< UV Sterilizer (manufactured bv Emperor Aquatics, Inc.) for 2 minutes.
3
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatic BioSystems, Inc. In-house Cultures
- 2. Age: 23.5-26 hours old <24-hours old Test Method Summary:
- 1. Test Conditions: Static. Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101 02/03/04 - 1509 EST 02/03/04 - 1147 EST UV Treated Outfall 101 02/03/04 - 1446 EST
- 7. Test Termination: (Date/Time)
Outfall 101 02/10/04 - 1521 EST 02/10/04 - 1150 EST UV Treated Outfall 101 02/10/04 - 1453 EST
- 8. Test Temperature: Outfall 101: Mean =25.00 C Mean = 24.70 C (24.6 - 25.3 0C) (24.4 - 25.3 0C)
Test Temperature: UV-Treated Outfall 101: IMean = 24.90 C (24.6 - 25.40C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for DH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinnevville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted February 3-10. 2004 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 98 98 90 90 90 90 Intake 100 100 1100 00 100 100 100 Test Solutions Mean Dry Weight (mg)
(% Effluent) (reicate number)
__________ 2 3 4 M ean Control 0.845 0.937 0.851 0.960 0.899 10.98% 0.942 1.069 1.064 1.068 1.036 22.0% 1.131 1.005 1.015 1.045 1.049 43.9% 0.932 1.027 1.006 0.994 0.990 72.0% 0.950 1.032 0.960 1.003 0.986 100.0% 0.941 1.147 0.925 0.893 0.977 Intake 1.058 1.057 1.090 1.027 1.058 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 5o: TUc = 100/ IC 2 5 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted February 3-10, 2004 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 2
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(%Effluent) D- -a ( l-e number) 1 2 3 4 5 6 7 8 9 10 Mean Control 31 32 27 28 31 33 27 32 30 29 30.0 10.98% 32 34 32 28 31 32 30 34 30 32 31.5 22.0% 35 31 33 36 33 39 34 34 29 34 33.8 43.9% 36 31 40 31 33 35 32 31 33 34 33.6 72.0% 34 29 29 37 36 35 36 37 29 34 33.6 100.0% 31 32 31 36 36 32 38 35 34 33 33.8 IC 25 Value: > 100% Calculated TU Estimates: <1.0 TUc*
Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC 5 0: TUc = 100/ IC2 5 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted February 3-10, 2004 using water from Intake Percent Surviving Test (time interval used - days)
Solutions 1 2 3 4 5 67
(% Effluent) 1 2 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
_ _ EData (replicate number
(%Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 33 32 32 32 33 34 29 30 30 30 31.5 Intake 34 38 32 34 28 32 34 35 28 36 33.1 IC 25 Value: > 100% Calculated TU Estimates: <1.0 TUc*
Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/ IC2 5 7
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted February 3-10, 2004 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 98 98 98 98 98 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 98 98 98 98 98 Test Solutions Mean Dry Weight (mg)
(% Effluent) replicate number)
( 2u3 4 Mean Control 0.856 0.925 1.007 0.924 0.928 10.98% 0.776 0.812 0.866 1.053 0.877 22.0% 0.942 1.061 0.939 0.832 0.944 43.9% 0.964 0.971 0.908 0.895 0.934 72.0% 1.052 0.965 1.079 0.968 1.016 100.0% 0.973 1.020 0.952 0.983 0.982 Intake 1.068 0.903 0.980 0.916 0.967 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Lower Limit: NA
- TUa = 100/LCso: TUc = 100/ IC 25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration I Toxicant Results (IC 25)l Pimephalespromelasl February 3-10, 2004 1400 7-days I KCI 655.8 mg/Li Ceriodaphniadubia February3-10,2004 7 1127 7-days I NaCl 1063.9 mg/L 8
PIIYSICAIICIEMICAL SUMMNARY Water Chemnistry Mean Values and Ranges for Pirnephalespromelas and Ceriodaphisia dubia Tests, Sequoyah Nuclear Plant EMuent (SQN), Outfall 101, February 3 - 10, 2004.
Test Sample ID Teprfr C Dissolved Oxygen (mgIL) PH (SUConductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final Oimhoslcm) (mgIL CaCO3) (mgIL CaCO3) Chlorine (mg[L)
Control 24.9 25.1 8.0 7.8 8.10 7.87 300 61 84
_____ 24.6- 25.3 25.0 - 25.3 7.8- 8.2 7.5- 8.0 8.02 8.21 7.73 - 7.96 287 - 307 61 - 62 84 - 84 -
10.98% 24.9 25.1 8.2 7.9 8.11 7.85 284 -
_____ 24.6 - 25.3 25.0 - 25.3 8.0 -8.3 7.6 - 8.1 8.03 -8.17 7.69 - 7.94 270 - 302- --
24.9 25.1 8.2 7.9 8.10 7.87 273 --
E 22.0%_ 24.6 - 25.3 25.0 -25.3 8.0 - 8.3 7.5 -8.1 8.02 - 8.17 7.70 - 7.94 262 - 289 - - - -
Q. 4.% 24.9 25.1 8.3 7.9 8.10 7.89 251 1324.6 - 25.3 25.0 25.3 8.1 -8.4 7.5 - 8.1 8.01 - 8.16 7.76 - 7.98 240 - 265 --
70% 24.9 25.1 8.3 7.9 8.09 7.91 222 - -
24.6 - 25.2 25.0 - 25.3 8.0 - 8.4 7.7 - 8.2 8.01 8.14 7.74 - 7.99 216 -233 - -
100.0% 24.6 25.1 8.3 7.9 8.09 7.94 192 71 82 <0.10 246- 25.3 25.0 - 25.3 7.9 - 8.5 7.4 - 8.2 8.02 8.13 7.82 8.05 184 - 198 67 - 73 80 - 84 <0Ol0 - <0.10 Inae 24.9 25.1 8.3 8.0 8.09 7.94 191 71 83 < 0.10 Inae 24.6 - 25.3 25.0 - 25.3 7.8 - 8.5 7.7 - 8.2 8.04- 8.13 7.74- 8.02 179- 197 68 - 73 80 - 86 <0.10 - <0.10 Control 24.4 24.6 8.0 8.0 8.l0 8.02 300 61 84 244 25.3 24.5 - 24.7 7.8 - 8.2 7.7 - 8.2 8.02 - 8.21 7.96 8.11 287 - 307 61 - 62 84 - 84 10.98% 24.5 24.6 8.2 8.0 8.11 8.01 284 - -
245- 25.3 24.5 24.7 8.0 - 8.3 7.7 - 8.3 8.03 - 8.17 7.92 - 8.10 270 - 302 - - -
20% 24.8 24.6 8.2 8.1 8.10 8.03 273 - -
20% 24.5 - 25.3 24.5 - 24.7 8.0 - 8.3 7.7 - 8.3 8.02 8.17 7.93 - 8.11 262 - 289 - - -
24.8 24.6 8.3 8.1 8.10 8.04 251 - -
49% 24.5 - 25.3 24.5 - 24.7 8.1 -8.4 7.7 - 8.4 8.01 - 8.16 7.95 - 8.11 240 - 265 - - --
20% 24.8 24.6 8.3 8.l 8.09 8.07 222 -
t24.5 25.3 24.5 - 24.7 8.0 - 8.4 7.7 - 8.4 8.0l - 8.14 7.96 8.13 216 - 233 - ---
1000% 24.8 24.6 8.3 8.1 8.09 8.09 192 71 82 < 0.10 "O% 24.5 - 25.3 24.5 24.7 7.9 - 8.5 7.8 - 8.3 8.02 - 8.13 8.00 - 8.16 184- 198 67 - 73 80 - 84 <0.10 - <0.10 Inae 24.8 24.6 8.3 8.1 8.09 8.09 191 71 831 <0.10
_ Int_____
ke__ 24.5 - 25.3 24.5 -' 24.7 7.8 - 8.5 7.8 -8.4 8.04 - 8.13 8 .00 - 8.16 179 - 197 68 73 80 86 <0.10 - <0.10 Overall temperature (CC) Average Mi1nimumn Maximum Pimephalespromelas 25.0 24.6 25.3 Ceriodaphidadubia 24.7 24.4 25.3 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the PinteplhalesproinelasTest, Sequoyah Nuclear Plant Effluent (SQN), UV-treated Outfall 101, February 3 - 10, 2003.
Overall temperature (CC) Average Minimum Maximum Pimephalespromelas 24.9 24.6 25.4 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected February 1-6, 2004, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalesprornelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Total Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in mg/L KCI or NaCl.
Sodium Chloride (NaCI crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
14
Sequoyah Nuclear Plant Biomonitoring February 3-10, 2004 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-February 6, 2004 I
2
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-February 6, 2004 3
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-February 6, 2004 4
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-February 6, 2004 5
Sequoyah Nuclear Plant Biomonitoring February 3-10, 2004 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD page -i- or-i-I I, Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):
Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Ashcville, NC 4 t peciry): Express Courier 28801 General Comments:
Facility Sampled: Sequoyah NP NPDES Number TN0026450 Phone: 828-350-9364 L., &O 0 Collected By: Wanda Allen, Ronnie Hlankins Fax: 828-350-9368 Field Identification Sample Description Grab/Comp. Collection Datefrimc Container Numnber &
Flow (MGD Rain, Event? ~
'e "2 o(4*~
ff-, r-4 .
Volume ) (Mark as Appropriate)
________________ ~Collected _ _ _ ~~X a ~ ~4 Date Time . Ycse If Yes, No Trace LgY Aniv;.;. . . Tn9 . 4k A ppe Indies ? r.'R ~ ... ~4 SQN-101-TOX Conmp V/104 -2/2/04 2(2.Sgai) NA _
SQN.INT-TOX Comp 2/1/04 - 2/2/04 1(2,5 gal) NA == =
- 3 Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Datc/Time Wanda Allen 0t 7e4K 2212004 Express Courier 212/2004 Ronnie Hankida . /mj O ___
A 7 - LVA eA UF Exprcss CoutiSi 2 ,xT, 2/2/2004 P _
o
?.-Oct
'*.53
_ _ _ 02-02 -64
_ . J(D Instructions: Clients should fill in all areas except those in the "Labormtory Use" block. Biomonitoring samples are preseved by storing them at 6 C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such n way that lihe laborutory will receive the samples with armple time to initiate testing within that time frame.
Samples shipped overnight on Friday via FcdEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Environmental Testing Solution, Inc.
Project Name: Sequoyah NP Toxicity 351 Depot Street.
P.O. Number NIA Asheville, NC Express Courier 28801 General Comments:
Facility Sampled: Sequoyali NP NPDES Number: TN0026450 Phone: 828-350-9364 L.., coa(
Collected By: Wanda Allen, Ronnie Hank-ins Fax: 828-350-9368 day,~
Field Identirication / I Grab/Comp.
Sample Description Instructions: Clients should ll in all areas except those in the 'Laboratory Use" block. Diomonitoring samples are preserved by storing them at 6C and shipping them in icc. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> froin the time of collection. Therceore, please collect annd ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Client: TVA Environmental Testing Solution, Inc.
Project Name: Sequoyali NP Toxicity 351 Depot Street P.O. Number N/A Ashville, NC ): Express Courier 28801 General Comments:
Facility Sampled: Scquoyalt NP
+ C*>.4ed~, L. 4j&A* -
NPDES Number: TN0026450 Phonc: 828-350-9364 L44t500o& CPIWW-4%--(
.oudA Collected By: Wanda Allen, Ronnie Hankins Fax: 828-350-9368 Field Identification / I Grab/Comp.
Sample Description Instructions. Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time orcollection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample timc to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Environmental Testiniz Solutions. Inc. Page .I of 6 Page 1 of6 Environmental Testirw Solutions. Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Sepuovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project#: iO(-12 Dilutionprearationinformation: Comments:
Dilution prep (°/.) 10.9S 22 43.9 72 100 Efluent volume (mL) 219.6 44 878 1440 2000 Diluent volume (mL) 1780A 1560 560 0 Totl volumc (mL) 2000 2000 2000 2000 2000 Test organism information: Test information:
Organism age:
- 23 m 2t, Q o Randomizing template: '4euoQ Date and times organisms G-zj.--Al 1C\OT QZO KS7- Incubator number 3.
were born between:
Organism source: AS, 6TxCA 62-OZ-04 Artemia lot number. t oo Transfer bowl information: pH = Temperature = ec Total drying time: ZY fl.
. PR 'Zq-3 Date/Timein: oZ-tbotl Y63O Average transfer volume: ID A mi Date /Time out: bz.4t.&q %,S?6c I_ Oven temperature: C Dailyfeeding and renewal information:
Day Date Morning Arternoonl Test initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time H RS j
-4 110t l 02oZ-oZc ya .cR l. dL.
t-oco'F 09di
.O'q5I I Lo 2.. i5 l3 i 6-0, O2qa-o.Icj-oo, 0.ORI09
.0- co 04§ O!; (Po o5 K--
2 nz-bsoq *() 9 4(.P IEZL o2.
0I' bz- q A20q.IsI' h.-
3 bz-bbo4 OCj15lD ti-ce 1150Z 6z-Oz-Ozq 0,4 D024. 18l0I I PlI:)-
4 -Oq-O~.q I t+ 0 1o Ib2 .YL.CA tqo20.ok l.oz 1
5 ___ O -f I I 10L1 lQ OL.cs Z .o4' o04tY.O1 I.Oz VIeA-6 oz-oqnY4 0qz t jo 150z oz oi.5X bti2c.. 1.03 L' Oz-.io .0 . 1521 ^,.','.*.*. *.. :-;,
Control information: Acceptance criteria Summary of test endpoints:
% M~ortality: ~ ~(VI s 209/. 7-day LCso ro Average weight per initial larvae: nRi!.! . .$. NOEC _I____
Average weight per surviving larvae: I.gq.? 20.25 mg/larvae LOEC > I /,.
_ ChV 1 Cl.x1 I Cli 'lco~d
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimephalespromelas Date: OR9 *D O '1 Client: TVA I Sequoyah Nuclear Plant Non-treated Survival and Growth Data Day CONTROL 10.98% 22%
A B C D E F G H I J K L oID to Io 10 (0 1o ID io ( /0o /o (o
/6 /Af__ 1_ /j '/6 /A /0 /6 /0
-D/ _ / /0 /6 Jo /0 / /- /A /0 i10 _IC_ 16 /0 10
_0 I)/6 /V/0 4 10 c) to to lID lO / 10 / t/.
5
__ J IL) mA) iJt / J) / /U lV /v 0( 10 lo lo) /0/ l I) / "/ 1 /0 /0 /0 /a 7
7 lb 0 ic lb 10 / f,/ /0 f0 i( _ /4 A - Pan welght (mgVW;&--UV - - -%,
B- Pan +Larvae weit Larvae vwelght (me) A A- B 8\ o \@ >Oa\! p Weight per Initial number of p, larvae (mga) , ~ ~ \1 . i~
-CIInitiatnumberoflarvne 0o O' 0 0 ___° Average weight per Initial number orlarvat(mg) 0. A(345 _ _ __
% ICcD -is. t-Calculations and *datareviewed: Ai_
d dta revie -161ed_"a Comments:
]Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimephalespromela.
Client: TVA / Sequoyah Nuclea ar Plant Date: c -6;S-Oq Non-treated.
Survival and Growth Data Day 43.9% 72% 4 100%
M N 0 P Q R S T U V W X r 0 D)JO)bl /t)1Dlo J /0 /0lc 10/0)/()
.1 lb ( /I JD' /0 /I /A /O /0 /0 /O
//
lb2 /O /1 /- b /0 d 2 10 1 ,a /06/ ( / /O 0 /0 9 3 /~QOIC /Z0l/Op C lo /O 0/O9 IC) 4 /d /c0 1j lo O 10_ (3 10 T_ d 9 10 6 (to/I lo 10/0/0/O :7- Ic 9 7
.6 . __o - _-__o 4-I A - Pan weight (mg) 1 - Pan + Larvae weight Larvae weght (mg) A- B
-i 'R Z ° _
\
-CInhitlnumberotlarvae I'D, \0 'O ' 0 Ov Average weight per Initial ,.qk number of arvae (ig) 0 .,11 i ez: -1b. eVo %6 m -- t.-o7s 7and daa r view 1ed Calculations and data reviewed: .e_
Comments:
"K.
i' Environmental Testing Solutions, Inc. Page 4 of 6 Eniomna Tet- Soin,:n.Pge4o Species: Pimephalespromelas I.
Client: TVA / Sequoyah Nuclear Plant Date: O0-2is-btI Non-treated t.. Survival and Growth Data I. Day 100% Intake Y Z AA BB 0
16 /'O lb 3 /6 16 16
/1
/104 0 /O 16 5
I.
r C.,I 6 /l 1to /6 /0 IV A - Pan weight (mg) - - -
I' I- B - Pan + Larvae weight Larvae veight (mg) - A - B T - C)
J4 Weigbt per Initil number or Uarvae(mg)
C/Initialnumberotklrvae \X \
Average weight per loltizl number of larvae (mCg) 8S Clculations and data reviewed:
Comm ents:
Environmental Testing Solutions, Inc.
Chronic Vhole Efluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: PNmephalt-spromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA ,SerayphNvelear Plst -1(ontrysttd Test dst: Fdtma 2004 ary3.19, lrejet umber. 1042 Reveheed b C.. e..a..a 7 R-V. 22q . hid.. t_ Vb fli.l h A- F.-'4 (eJ S. ICC* t A L~.' ,..C&CRS*saJ.. 4.l.&C./8.nA.. . L.....k................W.... I WWF.Z ........................... . 4i I.8 . i n4" .. _. 2..
_.i~ -4 (.) A.*I as.&.rh..(awi *.,4..r~fhi w._-___ ..(.< (V) asi.rhM e'-.aw. ** " jX
(_()O3 .. hdhO.) K) v A 12 10 14.656 23.11 *.454 0.845 0.t45 Control C t0 10 214.869 24.24 9.371 0.93 0.99 6.5 0937 100.0 0.199 6t3 Netapptlrabl D I1 10 14.71* 24.39 9.602 0.960 0.960 .-
E 10 10 14 921 24.34 9.419 0.942 0942 I'91% 20 t0 15.144 25.83 20 686 1.069 12036 6.0 20.0L0690 35 GC t 10 14.801 25.44 10.6 9 1.064 1.064 100.0 Lt36 &O *15 3 24 10 10 14.592 25.27 10.67a 1.063 _1.06 I 10- 10 14.521 25123 11.307 1.131 IM13 122 i 10 10 14.980 25.03 20.050 1LOOS 1.049 S.5 2.00 100.0 1.049 5.5 .16.7 2%K 80 20 14 553 24.70 10.147 2.O25 8.025 bI. t10 10 14.494 24.94 10.446 1.045 1.045 22 20 t0 15097 2442 9.323 0912 0.932 43.0% M 1t0 14.764 25 03 20 266 1.027 0.990 41 1.027 100.0 0.990 4.t .10.2 u 10 20 25.028 25.15 10062 1.2106 2006 I 10 10 14.510 24.52 9.940 0994 __0994 q 20 10 14 551 24 OS 9.497 0.950 0950 72% S t0 t 144368 25.29 10.322 1.032 0496 3.9 1.032 100.0 0.9S 3.9 .9.7 to2 20 14.642 24.24 9.591 0.960 0.960 tr0 10 14.765 24.79 10.025 1.003 I.0t)3 U t0 7 _ 14 S8 24.23 9.412 1.345 0.941 V 2%0 20 14 936 26.41 11.474 1.147 001. .14925 .TYi. .1.7 W 20 9 14.132 24.0t 9.24 1.028 1.103 87.4 2.247
.9.a so.e o.n0 X to 10 0 14.764 23.69 1 .926 0.891 0893 _
Y 10 1o 14 636 25.22 10.514 o.05t 2.052 I.e Itake Z t0o to 5.216 25.79 t0.574 057 LOS. LOS 1 2.5 °Oo.8 1.St i _17.1 aAA
- ii 10 10 10 20 14.119 2-4.523 25.79 24.7J9 10.902 .090 2027
. 1.027 0 .
10.265 _ _ _ _ _ _ _ _ _ _ _ _ _ _20 1_ _ _ _ _ _ _
Osafill lot, MSD - fiftuum Sivuilfkat Differeuce Usummett' MSD value: 0.1144 PMSD - Perem Minm Shfient Dillermc PmSD: 82.8 PMSD is a sarv..re oftest precisraL The PMSD is the ,rinrmnmpercent differece betwen the omtnl end tmawtt that cso be deelad statistically skpileant In a vbsole flhent mtkcity test.
On 2twp a *w irieaat di&ffernev oucu or H e~ammtal Testing Solutions. Inc. chmaic toxicity tets -Am a toxicant rdie .PI'equpoles gowth by 16.8%6ntth e emteol ed through nfereo loxlcset tt~ing.
vassetfs MSD vw¢al: 0.0624 IowerPMSDboundd tmnedbytUSEPA(IOthpersutic) -9.4%.
PMKs): '6.9 V PMSD bonl ddeniuiad byUSEIA(9th pucntil) - 3516 The low atd pp abndswe calcult ed by the tUSPAusing 205 tes codadd ront 19 ubortouic, oromephlea vvtb nkdhmuor o refec toxzant tests USEPA 2000. Undsntmdgsd MeoenkwgecrctISoJ Vabiliy b Whole SmuetTosicity o itesoslUdetheNt polluta sdrt Etinatlon EPA.133.R.0003. U 5 E tsl tet Or, C s tL E
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 213/2004 lest ID: PPFRCR Sanple ID: TVA/Sequoyah Nuclear Plant - Outfial I01 Nontreated End Date: 2V10/2004 Lab ID: US-lEnviron. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: RPA-821-R02-013 Test Species: PP-Pinephales prorelas Comnents:
Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 10.98 1.0000 1.0000 1.0000 1.0000 22 1.0000 1.0000 1.0000 1.0000 43.9 1.0000 1.0000 1.0000 1.0000 72 1.0000 1.0000 1.0000 1.0000 100 0.7000 1.0000 0.9000 1.0000 Transtornn Aresin Square loot Rank 1-Tailed Conc-% Mean N-Mfean Mlean mlin MaX CV% N Sum Critical D-Control . 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 10.98 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 22 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 43.9 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 72 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 100 0.9000 0.9000 1.2661 0.9912 1.4120 15.696 4 14.00 10.00 Ausllfary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <- 0.01) 0.48450965 0.884 . -1.8682549 10.6517865 Hquality orvariance cannot be confirmed Hypothesis Test (I-tail 0.05) NOEC LOEC ChV 71t Steels Many-One Rank Test 100 >100 1 Treatments vs D-Control sqn_02-03-04dafa
Environmental Testing Solutions, Inc.
Statistical Analyses Dose-Response Plot 1-0.9 0 0.8 0.7
.2 0.6 co 0.5 Q 0.4 0.3 0.2 0.1 0
co N N 0
.5: N fn 0) a}
0 0
6 sqn_02-03-04dafa
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish GrnwtIh and SurVival Test-7 Day Growth Start Date: 2/3/2004 Test ID: PPFRCR Sample ID: TVA/Sequoyah NuclearPlant - Outfall 101 Nontreated End Date: 2/10/2004 Lab ID: ETS-Environ. Testing Sol. SamPle TYPe: DMR-DiScharge Monitoring RepOrt Sample Date: Protocol: EPA-821-R02-013 Test Species: PP-Pimephales promelas Comments:
ConC-% 1 2 3 4 D-Control 0.8454 0.9371 0.8513 0.9602 10.98 0.9419 1.06S6 1.0639 1.0678 22 1.1307 1.0050 1.0147 1.0446 43.9 0.9323 1.0266 1.0062 0.9940 72 0.9497 1.0322 0.9598 1.0025 100 0.9412 1.1474 0.9248 0.8926 Transrorm: Untransformed 1-T,tiled Isotonlc Cone-% Mfean N-Mean Mean Min MaY CV% N t-Stat Cr1 hI2eal ASD Mean N-Mean D-Control 0.8985 1.0000 0.8985 0.8454 0.9602 6.535 4 0.9943 1.0000 10.98 1.0356 1.1525 1.0356 0.9419 1.0686 6.032 4 -2.883 2.410 0.1146 0.9943 1.0000 22 1.0488 1.1672 1.0488 1.0050 1.1307 5.452 4 -3.160 2.410 0.1146 0.9943 1.0000 43.9 0.9898 1.1016 0.9898 0.9323 1.0266 4.103 4 -1.920 2.410 0.1146 0.9898 0.9955 72 0.9861 1.0974 0.9861 0.9497 1.0322 3.889 4 -1.841 2.410 0.1146 0.9861 0.9917 100 0.9765 1.0868 0.9765 0.8926 1.1474 11.849 4 -1.641 2.410 0.1146 0.9765 0.9821 Autiliary Tesls Statistic Critical Skew Kurt Shapiro-Wik'S Test indicates nomil distribution (p >0.01) 0.93901283 0.884 0.86888714 1.48343183 B3artlett's Test indicates equal variances (P = 0.45) 4.75047112 15.O862722 Hypothesis Test (1-ta4l, 0.05) NOEC LO1C ChV TU MSDu MSDp MS13 MSE F-Prob df Dunnett'S Test 100 >100 1 0.11458425 0.12752838 0.01127405 0.00452112 0.06963139 5, 18 Treatments vs D-Control sqnO2-03-04dara
Environmental Testing Solutions, Inc.
Statistical Analyses Lmin~rhndtpobftlon (2010 Rts~aipl1es)
P.OW *. *SD 95% CL(Exp) SI&~W IC0s >100 lC10 >100 IC15 >100 1.0, 102O >100 0.9 105 >100 0.8 IC40 >100 0.7 IC50 >100 0.6 a 0.5 0
C 0.4
°L 0.3 0
a: 0.2 0.1*
nn 4.1
-0.2
. ....2 40.... 60........... .. .....
0 20 40 60 80 1co 120 Dog.%
Dose-Respome riot 1.2 I06-a0.8-I-tafl 0.05 leyc!
of significance 0 :
t0 -
0.4 I 0.2 CI 0
vm v.3 9
ao sqn02-O0104dard
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 2/3/2004 Test ID: PPFRCR Sample ID: 'TVA/Sequoyah Nuclear Plant - Intake Nontreated End Date:* 2/10/2004 Lab ID: ETS-Environ. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Samrle Date: Protocol: EPA-821-R02-013 Test Species. PP-Pimephales promelas Cormrnents:
Cone-% 1 2 3 4 D-Control 0.8454 0.9371 0.8513 0.9602 100 1.0584 1.0574 1.0901 1.0265 Transfonn: Untrnusformed I-Tailed Isotonic Cone-% Mean N-Mean Mean Mill Max CV% N t-Stat Critcal MSD Mean N-Mean D-Control 0.8985 1.0000 0.8985 0.8454 0.9602 6.535 4 0.9783 1.0000 100 1.0581 1.1776 1.0581 1.0265 1.0901 2.454 4 -4.971 1.943 0.0624 0.9783 1.0000 Auxilinry Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93903327 0.749 0.09935192 -1.4212827 F-Test indicates equal variances (p 0.21) 5.11299276 47.4672279 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE P-Prob df Hornoscedastic t Test indicates no significant differences 0.06238244 0.06942954 0.05094432 0.00206124 0.00252317 1, 6 Treatments vs D-Control sqnO_2.03-04dafa
Environmental Tcsting Solutions, Inc.
Statistical Analyscs Llnearlnterpobtfon (200 Resa11pIts)
Polnt % SD 95% CL(Erp) Skew 1o05 >100 C100 >100 IC15 >100 in .
IC20 >100 0.9-IC25 >100 0.8-1C40 >100 0.7.
IC50 >100 ,,
0.6 o 0.5-C 0.4-a 20.3
- a. 0.2-0.1*
-0.1-
-0.2-0 20 40 60 80 100 120 Dose %
Dote-Response Plot 1.2 I t n nl lo -
,, ,,. , .................. - *- - eof stgnificance 0.8 0
a~0.8 la br -
0.2-8 I
0 a
sqn 02.0/4'data
Environmental Testing Solutions, Inc. Page I. of 7 Environmental Testing Solutions, Inc. Page 1 of7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia Client: TVA Facility: Sequovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project #: 1042.
Dilution preparaioninformation:
- Comments:
Dilution prcp (%a) l i.9E 2Z l 39 1 2.. 1b0 ;
EMu(nt volume (mL) 2 ti. t I e lqqo z-O Diluent volurn (mL.) 11ft f ISbO I I -a 57O 0 Total volume (mL) 22Q2 2C) 7= .0.
Test organism information: Test information:
Organism age: 2 4-vkntls MD Randomizing template: 62ees Date and times organisms -a-o 83 m tole Incubator number and 2 b Z.
were born between: shelf location:
Organism source: 0V-zV- A-b YCT batch:
Transfer bowl information: pH -g. it Temperature =q.TY C. Selenastrum batch: At V1Z?-l Daily renewal information:
Day Date Test initiatIon, Control water Sample numbers Analyst renewal, or batch used used termination time Ml KS _ _
0 O7 L 11q1 O2.02-LA O42-& ci l-.09 4 2 0-x 054 1%3 vz a -S 64 c)4ZD. 1.5 _ .1 2 OC)Z-00,W llZOk OZ-e4 o'4ot'A.IB I 4 0 ~ZO-4-O1 O4DZOb~ol.Of _.07 6____ l-ao o2.-OID f wZo-oA 10.O32. di o2.-lob-tA~u
_ lo 0 wtC _ _ _ _ _ _ _ _d r' 4~
Controlinfor nation: - Il$2. Acceptance criteria Summary of tst endpots
% of Male Adults: r. s 07. l 20% 7-day LC50 :> I )/
% Adults having 3r' Broods: 16 1800%7. 0% NOEC IC<D
% Mortality: S20% LOEC > ico%
Mean Offspring/Female: 3 .05 2 15.0 offspring'ferale ChV I %
%CV: - <40.0% _ IC25 -
Environmental Testing Solutions, Inc. Page 2 of 7 Species: Ceriodaphniadubia Client: Senuovah Nuclear Plant - Non-treated Date: Oz,03.Oq CONTROL Survival and Reproduction Data
_ _ __ Replicate number Day 1 2 3 4 5 6 7 8 9 10 I Young produced ) l o O 0 Adult mortality b b _1.1I (I 2 Young produced _ 2 4- 6 t 8 9110 Adult mortalty -
3 Young produced Jj L lL LA I C). I C) S I C)
Adult mortality 1L. U. I ll - I-4 Young produced 0 LO c _ i Adult mortality tU > L L-u _ L %
5 Young produced 1F IC)I,Q) o I Adult mortality l Il l - LU l u L- _L-6 Young produced Adult mortality l q L tl.- l IL 7 Young produced ILO I 1 Totalyoung produced 3 3S l Z!I -a 3Z ..3b Z FlnalAdulrMortallty l- l tl X for3Broods e CI -
Concentration:
%Mortality:
% IM I Mean OffsprinFemale: l3o.0 l CONC: 10.98% Survival andReproduction Data Repilcate number Dy l I 2 3 l 4 5 6 7 8 9 10 I Young produced l 0 C) 0 L) 1(
l 2D 3
! Young produced Adult mortality Young produced l(
l IIJJ 2 C L 3
L(C...J l
U _ .
[
[
f.LI Adult mortality [ l - \.- l l - - l- -
4 Young produced l t4 l o o A I I Adult mortality L-l L- l '-1I_ -1 L- -L S Young prod i2- -l S1- IC) 11- = -0 6
Adult mortality Young produced j U
4f tA- X. -
-H-o V L- L .L i
l Adultmortlity - l --
lll L-
- youngY produced 17 TI T -C Isl2
= l J 3Z - -
L -
I 1-AS 1
{Finl Adult Mortality L U l - L - l =t l \I L-I Concentration: p r %Mortalir U l lMan Offivrinzalimale: lA I I % Reduction from Control: l-5. 0 I
Environmental Testing Solutions, Inc. Page 3 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: OZ-03 .oq CONC: 22% Survival andReproduction Data Replicate number Day I l 2 3 4 lS 6 7 8 [9 1D 1 Young produced O) O O t) O (w O C 0)I{B Adult mortality L U-i Youn produced ) __
__Adult morbiq...2( _l_(
_11ty _.t 3 Young produced LI Adult mortaUty t 4 Young produced Adult mortality J U l
U . L C S 127 S Young produced _ __
Adult mortality TL '- U- L L l \ l. 1.l 7 Young produced IL8 I24 10 1- 1. _ I I 1. _ f1 Tot6lyoung produced l35 3 l3C) 3 3 l l a0 l 301 lAC)ak Fina Adultl M ortality j. .. l. \ U. . U. l. 1. I I Concentration:
%Mortality:
l Mean Offspring/Female: I 3-. l
% Reduction from Control: lIZ.17 1 CO?'C: 43.9% Survival and Reproduction Data Replicate number _
Day __1 l_2 l 3 4 l5 6 7 8 19 10 I Young produced l j2 Il .. ... l° o 0 Adult mortality a - __ L~L l2 lYourg produced Adult mortaliy; l)l(. () Lt) )t( ) )tC 6 l
3 Young produced lAdult mortality
(
k- lL
-5 k
Is . Is 4 Young produced C)j...
O j i s _I I 0 s Adult mortality Young produced Q l lU U. l iL C.)U IICU. 0 0U.
Adult mortality L l t U. .I L Young produced j j l cl O 0 0 I
l6j A - - a a jn- --
l7 Youngproduced l1 l 1 l _ e18j l 0 I __ I_
lTotalYoung produed l 31 l '1O 31 33 l 5 l1 l Adult Mortality l . L I. LLl.. l l I i Concentration:
% Mortality: 0 l l Mean Offspring/Female: I 35.lo I
%Reduction from Control: -12.6 I
Environmental Testing Solutions, Inc. Page 4 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: 02 s 3-co cONc: 72% Survival and Reproduction Data
_ Replicate number D _Y_I I 2 3 4 5 6 7 8 9 10.
I Young produced I-j (f> O O 0 _ O Adult mortality I LI G-2 Young produced D5 o o 0 O Adult mortality LI LII _lLI L 3
4 Young produced Adult mortality Young produced l u = s_.
l S
I ()
- e - '
C)
CO 5-
_A S
Adult mortallty _
Sl Young produced I 10 I I. lt l I I.12- .+/-2.
Z Aduit mortality 1.'. u lYoung produced l C) C) C l CA ) .
Adult mortality l --
L..... L- l tl l1 *l . 1 7 l Youngproduced I8 I LA I Ito 18 II zol CS I Totalyoung produced l 34 l 2° l Sri 3 5l 3(o l Ilz l 3'4 Final Adult Mortality G l . l L l- \ lT j Concentration:
%Mortality:
l Mean Offspring/Female: T33.(
% Reduction from Control: 12.0%
CONC: 100% Survival andReproduction Data Replicate number Dav IT 2 1 3 41 5 6 7 8 9 I1 l I Young produced DQ l o2 l l. 0 ) 2 Adult mortality (_ ( L l L 1l j ll 2 Young produced l - l IOIL) l .) ..JVl() l
- Adult mortality L- C,l[l .-
3 Young produced l 1 IIl l4 J2_ ' l 1 0T 0
Adult mortality l \ l l l l -
- jay pAJ 10 or -dut a--aL- aL-4 6
5 Young produced Young produced Young produced Adult mortality 1l ID O
C)0 -
l l--
C)l
- l O2.I.
l C) tL4 1.2-i~(0l Z *-c--C 1~..
) C
~..I2
)
Q-~
l 7_Yong_
Total young produced podued_1_I' 313 32 l3a(. l3 Is 3 I-- I -
. 8 I
1 q 33 Final Adult Motlity _ I u l l l l 1 I i I Concentration: I I %Mortality:__
I Mean Offsorine/Femalt: 133.& I I % Reduction from Contzol: l -12.1 71
Environmental Testing Solutions, Inc. Page5 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: pz-c3 oC4 CONTROL Survival andReproduction Data Replicate ounmber Dy _Il 2 3 4 5 6 7 8 9110 Youngproduced __
l Adult mortulity unproduced 2 G IL 6 0 t O lL lQ 1.
3 Young produced S 0 5 Lq Adult mortalllt L L L I - L, 4 31 Young produced produced C) l 5 C)- -0 iO
- I - - 0Young LA__ t r C)
Adult mortality _ L- L - t_ L_ _ _
6 6
I Young produced Adult mortality Youngproduced l l
I I . U 10 to L
II U
1 '
C) tC j
C)
U 10
( t L0 L 6
StT a
F Adult mortality l t_ . l U. * .-
I -
I2e I 6 7 rYoung produced tk t0 16 1,t L ,, ,ciI> %t ,0l, 1 Totalyoung produced l 3 bz 53 3Z a3t 21 3O C, 3t a nal Adult Mortality - - - L
- t. I l Concentration:
%Mortality:
Mean OffsprinW/Female: I 31.S l Reduction from Control:
CONC: 100% Intake Survival andReproduction Data Re licate number I D 1 2 3 1 4 5 6 7 8 I 9 10 T0 I
_Y I Young produced o _
Adult Mortality L~ L JcL £ il - U- _
2 Young produced l 2. l Adult mortality I (. l L 3 Young produced 1 j - ..O.. - -I___
Adult mortality l U l L l U.l L l l J _
4 Young produced l O S Adult mortality U-- \-..L- L-.
6 Young produce1 Adult mortality 1oung produced L*
C) Q c) 1
. } .
10 L
0 I
+ I0 4Q CAPM 0-ieL.
] Adult mortality lLl . U- IL I 1 L I I . L 7 Youngproduced l , g 1 I4 Totil youngproduced jT l 3\ 3L1 2l l32 324 SS l 2 3c Final Adult Mortality U...L L L- L1T - t7= ?
I Concentration:
%Mortality: I- I Mean Offsprinz/Female: 33. 1 I % Reduction from Control: i - . IV 7, I
Environmental Testing Solutions, Inc.
Verification of Ceriodaphnla Reproduction Totals Control-1 72%
Day Replicatenumber ol layepliate number - Total 121314 15 6 718 9 10 Dy 1 2 34 54 6 1 3 19 10 I 0 0 0 0 0 0 0I 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 o 0 0 0 o0 O 0 3 4 4 4 4 0 0 5 6 0 0 27 3 5 5 0 6 0 5 6 5 4 4 40 4 0 0 0 0 4 6 0 0 5 4 19 4 0 0 5 0 -6 0 0 0 0 0 21 5 11 12 10 11 11 12 10 10 10 101 107 S III 10 10 12 14 12 12 12 10- 13 116 0 0 0 0 0 0 0 0 0 0 -' 0 0 0 00 _ _
6 0 7 16 16 13 13 16 15 12 76- 15 15 147 7 i8 14 14 19 1618 182 Is 1 6 TIotal 311 321 27 281 31 33 27 32 30 29 300 To tat 34 29 29 37 36 35 363 29 3 3 10.98%
Dayeepliate Day 3 5 numbtr 6 17 8 9 110 Total Day lIt pIrleatnumber - Total 5 6 7 810 1 0 0I 0 0 0 0 I 0 0 0 0 ___0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 5 0 5 5 4 6 4 5 4 4 42 3 S 4 0 S 4 4 0 4 5 .5 36 4 0 4 0 0 0 0 0 0 0 0 4 4 0 1 4 0 0 0 4 0 0 0 9 5 12 11 to 10 2 I2 11 13 10 121 111 5 20 12 12 14 IS5 13 14 13 0- 125 6 0 1 0 0 0 0 0 0 0 0 1 -6 0 0 0 0 0 0 0 0 0 7 is is817 1 IS 16 15 161 16 :1 6 157 '7 16 1 s 157 17 I15 20 18 1.9 161 168 Total 32 34 32 281 31 321 301 34 30 1321 315 Total 313231 3 36 132 381 351 341 33 338
'22% Control-2
'Iletate number To___ Day ~Itt ilicaternumberToa DaRe Da 1 w. 3T 4 7 18 9 Tota I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 o0 0 "Too 0 0 0 0 0 2 0 0 000a 0 0 0 0 0 0 3 4 5 0 6 5 6 4 -5 5 0 40 S 0 5 4 5 4 4 4 3 0 34 4 0 0 6 0 0 0 0 0 0 S 1I A____ 0 4 0 0 0 0 0 0 0 4 8 5 13 12 20 13 12 14 11 11 10 12 118 5 12 2 10 30 11 12 It 10 t0 12 10 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 20 0 0 0 7 is 14 17 17 16 19 19 1 14 17 169 7 61 16 6 718 14 1 71 6 Tot-al 351 31. 33 36 33 39 .34j.34 29 341 3 43.9% 100% Intake Day ~~Itt heat.0 number oa Day it pIteate, number- Total Dy 1 2 3 4 S 7 8 9* 10* Toa 12 3 4 5 6 7 R 9' 10 I 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 6 0 6 0 4 S S 4 5 40 -s4 5 5 5 0 4
4 0 0 5 4 0
4 0
3 0
0 S
35 9
4 0 S 0 S 0 0 0 0 0 0 10 0 0 0 5 13 10 14 1O I11 13 10 10 12 10I 11 S 11 't4 12 12 10 10 14 13 30 11 117 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 1 1 1 16 2 16 li 71W6 1 1 16 1 173 7 18 19 15 18 14 17 16 18 15 19 169 Total 36 31 40_ 311 3.3 35 312_31 331 34 336 TOta! 34 18 2 3=4 1281 32 34 35 281 36 331
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodapihniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant1 Nontreated Test dates: Febru2ry 3-10, 2004 Project number- 1042 Revelwed by: 4 a '4 Concentration _ Replicate number Survival Average reproduction Coeffiiednt of Nrceot reduction (ton
(%) - 2 3 4 5 6 7 8 9 10 (/) (offspring/femrle) Vatrition (V-) pooled onno (%)
Control - 1 31 32 27 28 31 33 27 32 30 29 100 30.0 7.2 Not applicable 10.98% 32 34 32 28 31 32 30 34 30 32 100 31.5 5.8 -5.0 22% 35 31 33 36 33 39 34 34 29 34 100 33.8 8.0 -12.7 43.9% 36 31 40 31 33 35 32 31 33 34 100 33.6 8.4 -12.0 72% 34 29 29 37 36 35 36 37 29 34 100 33.6 9.9 *12.0 100% 31 32 31 36 36 32 38 35 34 33 100 33.8 7.1 -12.7 Control .2 33 32 32 32 33 34 29 30 30 30 100 31.5 5.2 Notapplicable 100% Intaks 34 38 32 34 28 32 34 35 28 36 100 33.1 9.7 -5.1 Outfall 101: MSD = Minimum Significant Difference Dunnelt's MSIT)value: 2.650 PMSD - Percent Minimum Significant Differenct PAISD: 8.11 PMSD is a measure of test precision. Tie PMSD is the minimum percent difference betweer the control and treatment that can be declared statistically significant in a whole efuent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when intake: a toxicant reduces Ceriodephinia reproduction by 10.1% from the control.
Dunnett's MSD value: 1.981 Lower PMSD bound determined by USEPA (1 0 1b percentile) - 11%.
I'MSD: -6.3 Upper PMSD bound determined by USE-PA (90"' percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerfodaphnla reproduction in chronic reference toxicant tests.
USEPA. 2000. Understatling and Accounting for Method Vauribility in Whole Emuent Toxicity Applications Under.the National Pollutant Discharge Elimination Program EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.
Environmcntal Testing Solutions, Inc.
Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 213/2004 Test ID: CdFRCR Sample ID: TVA/Sequoyah Nuclear Plant - Outfall 101 Nontreated End Date: 2V1012004 Lab ID: ETS-Environ. Testing Sol. Sample Type: DMR-Discbrge Monitoring Report Sample Date: Protocol: EPA-821-R02-013 Test Species. CD-Ceriodaphnia dubia Conunents:
Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 32.000 27.000 28.000 31.000 33.000 27.000 32.000 30.000 29.000 10.98 32.000 34.000 32000 28.000 31.000 32.000 30.000 34.000 30.000 32.000 22 35.000 31.000 33.000 36.000 33.000 39.000 34.000 34.000 29.000 34.000 43.9 36.000 31.000 40.000 31.000 33.000 35.000 32.000 31.000 33.000 34.000 72 34.000 29.000 29.000 37.000 36.000 35.000 36.000 37.000 29.000 34.000 100 31.000 32.000 31.000 36.000 36.000 32.000 38.000 35.000 34.000 33.000 Transrorm: Untransfonned _-Tailed Isotonie Cnnc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD hfean N-Mean D-Control 30.000 1.0000 30.000 27.000 33.000 7.201 10 32.717 1.0000 10.98 31.500 1.0500 31.500 28.000 34.000 5.844 10 -1.295 2.287 2.650 32.717 1.0000 22 33.800 1.1267 33.800 29.000 39.000 7.988 10 -3.280 2.287 2.650 32.717 1.0000 43.9 33.600 1.1200 33.600 31.000 40.000 8.441 10 -3.107 2.287 2.650 32.717 1.0000 72 33.600 1.1200 33.600 29.000 37.000 9.940 10 -3.107 2.287 2.650 32.717 1.0000 100 33.800 1.1267 33.800 31.000 38.000 7.084 10 -3.280 2.287 2.650 32.717 1.0000 Auxiliary Tests Sthlistic CriUcRl Skew Kurt Kxlnmogorov D Test indicates nornal distribution (p> 0.01) 0.6445598 1.035 0.07266985 -0.2130522 B3artletts Test indicates equal variances (p - 0.59) 3.74 186516 15.0862722 Hlypothiesis Test (I-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob dr Dunnett's Test 100 >100 1 2.64956744 0.08831891 25.5366667 6.71296296 0.00503867 5,54 Treatments vs D-Control.
sqn_.02-03-04data
Environmental Testing Solutions, Inc.
Statistical Analyses Linwer Yetelpo1tfon (200 Rtesea")
P.1"t % SD Is. CT. Skew Icos >100
)CIO >100
'CI5 >100 1.0 1C20 >100 0.9*
IC"5 >100 0.8*
1C40 >100 1C0S >100 0.7-0.6-I, 0.5-04-C 0.3 0.2-0.1-
.0.1
-0.02I ... .... ...
0 20 40 60 00 100 120 Dose %
Dose-IRtspome Plot 40 -
35:
I I e 30 1 t 25-
- ... i. I
............................................. 1-tail, 0.05 ftvd ofslgnlflcance z a:
10 '
5-0 d
rs 8 7
sqm_02-03-04data
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodsplinla Survival and Reproduction Test-Reproductlon SEtI Date: 2/3/2004 Test aD-. CdFRCR Sample ID: TVA/Sequoyah Nuclear Plant - Intake Nontreated End Date: 2/10/2004 Lab ID: RTS-Environ. Testing Sol. Sample Type: DMR-Dischargo Monitoring Report Sanple Date: Protocol: rlPA-821-R02-013 Test Species: CD-Ccriodaphnia dubia Corrfnents:
Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 33.000 32.000 32.000 32.000 33.000 34.000 29.000 30.000 30.000 30.000 100 34.000 38.000 32.000 34.000 28.000 32.000 34.000 35.000 28.000 36.000 Transform: Untransformed i-Tailed Isolonic Cone-% Mean N-NMean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 31.500 1.0000 31.500 29.000 34.000 5.238 10 32.300 1.0000 100 33.100 1.0508 33.100 28.000 38.000 9.706 10 -1.401 1.734 1.981 32.300 1.0000 Auxiliary Tests StUMstic Critical Skew Kurt Shnpiro-Wilks Test indicates normal distribution (p > 0.01) 0.95019853 0.868 -0.4564349 0.47545684 F-Test indicates equal variances (p - 0.06) 3.79183674 6.54108953 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df llormscedastic t Test indicates no significant differences 1.98051357 0.06287345 12.8 6.52222222 0.17824842 1, 18 Trcatments vs D-Control sqn_02-03-04data
Environmental Testing Solutions, Inc.
Statistical Analyses LUnt.rerpolition (200 Rrniel)
Point %
SD fat' CL Slw
- r~n _ t SV I
>IUV
'Cia ICIO >100 ICIS >10l 1.0,
. IC20 >100 0.9 1C25 >100 IC40 >100 IC50 >100 O.G 0.5-
,,S 0.S*-
0.4 o 3-0.2-0.1-u.u - ------------
-0.1 -
.0.2 ..
0 20 40 80 80 100 120 Dose %
Dose-Reorme Plot
-u 35 -
30- 1.tal, 0.05 level
- of slgrfince C
o 25-X20 10 -
01.
I 0
snO2-03-O4data
Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1oC6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility; Secuovah Nuclear Plant - UV-treated NPDES #: TN 0026450 Project #: 10pC Z -
Dil re ration information: Comments:
Dilution prep (%/.) 10.98 22 43.9 72 n 0 Muew volum(ml.) 219.6 . 440 878 1440 2000 Diluent volumc (rL.) 1780.4 1560 1122 560 0 Total volume (mL) 2000 2000 2000 2000 2000 Test organism information: Test information:
Organism age: Z3.2S yo Ts.nS- I4ouvS pQtL Randomizing template: &Ue-Date and times organisms o0.-O2 -oq 1IM-M rt o Incubator number.
were born between: . tsr Organism source: AKS 02-02 -( Anremia lot number:.
Transfer bowl information: pH Temperature- DC Total drying time: Pt f-C
____ __ Date/ Time in: C) 1I IuW7 Average weight per initial larvae: . , j. NOEC Il 7 I Average weight per surviving larvae: 1) . k 0.2s mgnarvae LOEC _>JP0 ChV I >_CO'ld IC?.4 2
Environmental Testing Solutions, Inc. Page 2 of 6 Environmental Testing Solutions, Thc. Page2af6 Species: Pimephalespromelas Date: OA.p514A Client: TVA 1Sequoyah Nuclear Plant UV-treated Survival and Growth Data Day CONTROL - 10.98% - 22%
A lB C D E F G H I J K L
- 2 - - / /0 /0 /0 /0 /0 /A /0 I o/6 6 1/ Ab 1 / /0/0 /06 6 /0 /0~
2 /OL ) / /0) A0 /0 / /6/0/6 /D/0 l3 /to "6 lo I( 10j/0 qd10 /0 la 310 to lo_ C 10 10 10 SC to 0 _
IC) o 5 £ 0 I6 lo to o(3 10 to 9 Ag) l8a
, 10 /0 ,4 /0 I. 0 , (e_/0 ,o 7 hDi 1 I/Oi IC3 jo I &)tbIo 9 I A-Pan welght (mg) K - - - - -0 - -
13- Pin + Larvae wveight (4s (mg) .5O1 fi6° b' N li__ __p._ *_'16~ .~
Larymewtight(mg)-A-13 40y i i5)h<G _ R~ v6aS_ c?
Wetiht per Initial number or larvae (mC)lb%~
Cl/litialnumberoflarvae tr 0O \ 0 O* i \ . \*
Average weight per Initial number orlarvae(mg) o5 ,2 o.8i1 o .1t4 07,_M. 5.S70 old f-C 1,17 Calculations and data reviewed:..,~!y Comments:
I Environmental Testing Solutions, Inc. Page 3 of 6 Species: Pimephalespromelas I Client: TVA I Sequoyah Nuclear Plant UV-treated Date: _l> Q I
I I Comments:
Il I
I
Environmental Testingy Solutions. Inc. Page 4 of 6
-sn -
Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant Date: o.tb5-,O9 UV-treated Survival and Growth Data Day 100% Intake Z
_ AA
_Y BB o /. /6 10 /0
/6 lb /a /0 2 / /,O. '
3 /O Io q 4 it 1) 9 R) to it) q7 6 M /6 i&
A - Pan wel-igt (mg) - -
B - Pan + Larvae weight LarVae welght (mg) - A - -
Wclglt per Initial number or Iarvae(mg) O aP O,\.\
CIIual triumberoflary2e \ O _ O' Or Average wefght per Iflit2l _
number oflirvaC (mg) . ..
Calculations and data reviewed: A-Comments:
Environmental Testing Solutions, Inc.
Chronic bhole Emuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephatepromdas Quality Control Verlflcation of Data Entry, Calculations, and St2tistlc2l Anilyses C1ient. 'TVA, Sequyah Nthaer Mlat U IV trested Tnt datew Fretiry 3-10, 2004 Project lwber 1042 Iterdwed A i r cA.-.ss A Leach-.1t.-V WOCNISr.l-*n Prn.dlewt I _ e MUChatdsn M.-b..nll Cdej. t . 'nd.a I-e
__ 1-C Q33I (t0 -A-1 1 Ct .bw OA) lt__-bof A t0 10 15.116 23.61 *.564 0.t56 .O1t6 C C tO_10 _ _ 14.061 t 24.10 9_.49 0.925 0.928 6A 0.925 100.0 0.91t 66 Not epplIabk D 10 to 14.693 23.93 9.237 0.924 0.924
- 9. 10 it 14.306 22.37 7.764 0.776 0.716 36.90% C 10 10 14.643 22.76 3.166 00. 0.77 14.0 0..O12 _00.0 0.77 14.0 is to1 30 15.026 23.69 8.664 0.86"I 0.066 H 10 0o 14.634 2S.1l 10526 1.053 o 1.053 t o0 9 14.527 23.95 9A23 3 047 - 0_942 212% J to to 1 4.667 25.28 10.613 1.061 0.970 11.0 1061 97.5 0.944 9.9 .7 K i0 30 14.996 24.39 9J94 0.939 0.939 L 30 l0 14.796 23.12 t.324 0.tl2 O.U2 M I0 10 14.632 24.217 9.63t 0.964 0.964 419% N 10 . 0 IS.349 243;6 9.713 O901 0.934 4.1 0970 100.0 0.934 4.1 .0.7 o 10 30 14.593 23.67 9.077 0M90 0.900
.P 30 10 14.364 23.11 *.946 0.95 0.893 I0 t0 14.59 25.52 10.521 1.052 1.052 72 K 10 10 14.576 24.23 9.634 0965 1.016 5.t 0.96 100.0 1.016 5.7 -9.S S t0 I30 13.029 25.02 10.791 .7 .1 T 10 10 14.323 24.20 9.675 0.96t 0.96t V 30 10 35.043 24.78 9.732 0.973 0.913 100% V 10 3o0 14.t73 25.07 10.197 1.020 0.9t2 2.9 1.020 00.0 0.91 2.9 .5.3 W 10 10 14.734 2425 9.516 0-952. 0.95t X 30 10 14.911 24.75 9.t32 0.9t3 0.93 30 10 15.056 25.74 10.684 1.06t 1.063 00%3take Z.
20 I0 31.601 23.64 9.033 0.903 o.99 7.0 0.903 97.5 0.967 7.0 -4.2 AA 1II0 3 34 ..2 2465 9.79- - 0.980 0.980 138 I30 9 34.436 23.65 9.364 3.038 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _0 9 6_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
ownh t0l! MSD - Minmita Sl16tcat DiIfnice I)t's8 MISD sahts 0.3271 PMSD - Peernt MiKalnr Sivoicant Di~frewe rMSM, 33.7 PMS[isa ueuue offest precbs;o."th PM5D Is de "tebmu riuzut tino d1irnct between the conro had btbmftothat C" b derbied UIttstisaly lsgil6tnlt In0 wholk ethIlu SoxleIly tcLSOs aswme. a si,3uscant difference occrs AoCEnvironmttal Ten~g Sottlo,, Int.chduoktoikyteytesu when a totlmit robeet Pflnephalu xrowth by I6.3% fOom Oe toatolo (detenuitd tWiou cflxtce toxkinc tesdog).
DiMe1tt MSD "1el: 0.4045 Lower PMS boi Adeimnised byUSEPA(I30h Mpitcatbif) - 9A%.
tMSM, 30.2 Uppet PMSD boand detcned by USEPA (906 percendk) - 35%.
Tte lower anduppwrbowds wte catkd bydic US EsA
- sit203 tets wduettd fomn 19tbo'tott it rIrYwpJepa i t croio crweereace tosxicul teits.
USSPA. 2000. Ud&n-adix audAccounnC (of Metd Vahbitiy nWlVoo Eflent ToxeityAeppUtetns Uoder y Nataonsal Pollut Distcue EcuinadorPrown. PEA-2t3-RA-. US Ensvoevrentadl rotedo sAgeeyCatililatd. OIL
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 213/2004 Test ID: PPFRCR Sanmple ID: TVA/Sequoyah Nuclear Plant - Outfall 101 UV-treated End Date: 2/10t2004 Lab ID. ETS-Environ. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: Protocol: E-PA-821-R02-013 Test Species: PP-Pirnepbales pronmlas Conrnents:
Conc-% 1 2 3 4 D-Control 0.8564 0.9249 1.0068 0.9237 10.98 0.7764 0.8117 0.8664 1.0526 22 0.9423 1.0613
- 0.9394 0.8324 43.9 0.9638 0.9711 0.9077 0.8946 72 1.0521 0.9654 1.0791 0.9675 100 0.9732 1.0197 0.9516 0.9832 Transform: fntransformed I-Tailed Isotonie Conc-% Mean N-Mean Mean Min Max CY% N t-Stat Critical MSD Mean N-Menu D-Control 0.9280 1.0000 0.9280 0.8564 1.0068 6.633 4 0.9468 1.0000 10.98 0.8768 0.9449 0.8768 0.7764 1.0526 14.020 4 0.971 2.410 0.1271 0.9468 1.0000 22 0.9439 1.0171 0.9439 0.8324 1.0613 9.908 4 -0.302 2.410 0.1271 0.9468 1.0060 43.9 0.9343 1.0068 0.9343 0.S946 0.9711 4.149 4 -0.120 2.410 0.1271 0.9468 1.0000 72 1.0160 1.0949 1.0160 0.9654 1.0791 5.738 4 -1.671 2.410 0.1271 0.9468 1.0000 100 0.9819 1.0582 0.9819 0.9516 1.0197 2.895 4 -1.024 2.410 0.1271 0.9468 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk'sTcst indicates norinl distribution (p >0.01) 0.95740587 0.884 0.75709063 1.10743652 nartlett's Test indicates equal variances (p - 0.22) 7.06173706 15.0862722 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChnV TU MSDIu MSDp MSlt MSE F-Prob df Dunnett's Test t00 >100 1 0.12705863 0.13692401 0.00915971 0.0055591 0.19831203 5, 18 Treatments vs D-Control sqn._02.03-04data
Environmental Testing Solutions, Inc.
Statistical Analyses Unear Interpolation (200 J2anaplt)
Point % SD 9S% CUErp) Skew C035 >100 IC10 >100 MC'S >100 1.0 IC20 >100 0.9' IC25 >100 0,8*
1C40 >100 0.7 IC5O >100 0.6' w0.5
- a. OA -
0 A a 0.3.
0.2' 0.1' 0.0-A
.0.1-
-0 2..' ............ ..... ..
0 20 40 s0 80 100 .20 Dole %
Dosn-Respanse Plot 1.2 1' . ........
I. .......... ...... .IL--
..................... 1-aD, 0.05 level 0
Of pIrfncance 4 0.4 0
' OA 0.2 a
-a 4.4 at 4.4 01 a
sqrn02-03-O4data
Environmental Testingc Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Piephanltespromnelas Daily Chemical Analyses Clie.t: Selt.,,ioyahl Nuclear Plrt Nan-Trealed Test ifles: refll inry 03 . 10, 2004 P'roject nisituher 1012 llewell ly I ,. 0 Cnicecgraflfio Pararimeler I)a 0 Day I Day 2 I)a 3 Da 4 1)S D5ya6 1111al nl itial IFilnal .Inital r FI ial IInitial IFial nlal I Filial Initifa Fl Initial inal pl I (SU) X.09 7.88 8.02 7.94 8.05 7.'J6 8.12 7.80 8.11 7.96 8.21 7.NJ I.l0 7.73 DO (ntsg0.l) 8.1 .0 X.0 7.8 8.2 7.8 7.9 7.7 8.2 7.9 7.8 7.5 7.8 7.8 Ctnu ctlivity (vnitocs/en) 298 301 305 295 307 305 287 Conlrol Alkalinily (mg/L CaCO,) 61 _62M 61 lamIlbess (19lmgCACO) 84 84 84
_rcnupertiure (IC) 25.3 25.2 24.9 25.11 25.0 25.3 24.7 25.0 25.2 25.3 24.6 25.1 24.9 25.0 pHi (SU) 8.13 7.83 8.1 1 7.90 8.03 7.94 8.09 7.91 X.0 7.93 8.17 7.78 8.12 7.69 DO (93igfl-) 8.3 8.3
- 8. 7.8 8.3 7.9 8.0 8.1 8.2 8.0 8.1 7.6 8.2 7.
10.98% Condunctivily (jsniinskenl) 27S 279 _ 302 282 i _ 294 283 270
. Tetiilerattire (C) 25.3 252 24.9 25.1 25.0 25.3 24.7 25.0 25.2 25.3 24.6 25.3 24.9 25.0 II I(SU) M.I 7I9 8.3 7.89 8.02 7.94 8.0 7.91 8.08 7.93 8.37 7.77 8.13 7.70 M) (nlgh.) 8.3 X X8.3 7.91 8.1 7. 8.0 7.9 8.3 7.9 1.3 7. X8.3 8.0 22% C41nitn1tlsivily (11inIlnstlcnil) 270 275 289 263 27X 271 _26 Tesnuperateire (C) 25.3 252 24.9 25.3 25.0 247 50 25.2 25.3 24.6 25.1 24.9 25.0 1ll (SU) X.l1 I 7.98 l 7.X8 8.0 7.91 8.09 793 8.08 7.') 8.16 7.85 S. Il 7.76 43310 ("Dmr/l.) 8.4 8.1 8.4 7.9 8.1 7.1 7.9 8.2 7.8 8.2 7.5 8.4 8.0C Condiuetivity (11nluos/eli ) 249 256 265 246 25f 243 241 Temlperature (C) 25.3 252 24.9 25.3 25.0 2S.3 24.7 250 25.2 2S.3 24.6 25.1 24.9 25.0 p11 (SU) . 8.1 7.98 8.11 7.88 8.01 7.3 8.09 7.96 8.09 _ 7.99 8.14 7.88 8.0' 7.74 3)O (nIght..) 8.3 8.2 8.4 7.7 8.1 7.8 8.0 7.9 8.3 7.8 8.3 .4 A7.7 8.1 7C2o% CnuulnctIvity (nInulushs/emi) 220 __ ,22' 217 216
_ emperatiure
_ _ ('C) 25.2 25.2 24.9 25.1 25.0 25.3 24.7 25.0 25.2 3 24.6 25.1 24.9 25.0 fill (S3I) X.12 X.O5 8.11 7.94 8.02 7.98 X.07 8.01 8.l0 7.99 8.13 7.82 X.08 7.X2 iO (muigr/ilj X.4 8.2 8.41 7.6 7.9 X .( 8.2 8 .0 X.? 7.9 8.3 7 .4 8.5 .K Conductciivity (tjnnillswecm) 192 1 _ 1191 19X IX!
100o AlkalUnIty (mn/l. CaCO,) 72 7_
Irlamesi (migL. CaCO 3) 82 84 Tcotal Resdisal Chleprille (ngIl) <0.10 <0.
Temperatre CC)
_r_ 25.3 25.21 24.91 25.1 25.0 25.3 24.7 2 25.2 253 24.6 I 25 24.9 25 IIl (SU) 8.31 81 .) 8. I3 7.93 8.04 8.03 8. 8.33 8.)2 8.33 7.84 8.07 7 i)t) img/L.) 8.4 .2i 8.5 7.7 7.1 8 M. 8.4 8.0 8.5 7.7 8.5.
Cu'nuluctlvlty (ganuau/nm) 3'90_ I'71)IY19UX) 100% Inlake Alkalhuilty (nigflCaCOC ) 72 Ilurdlness"(m/l CaCO3) 86 T'optal 3&esiuual Chlorine (nigfL) <0.30 TIemnpermnbre C.) 25.3 25.2 24.91 25. 25.01 2.5.31 24.71 25.0 25.21 25.31 .24.6 1 25.13 24.) 25.0
xnvronental Testing Solutions, Inc. Page 5 of 6 t1Species: Pintephtalespromelas Date: 0,2 -God t4 Client: TVA / Sequoyah Nuclear PlaiInt - Non-treated
>'DailyChemistry:
Dav Analyst KW- LQ CU _CA Concentration Parameter .1 U,; _1 WtS ,^ 111S£ CONiTROL pH (S.U.) IjO 5 8 .02Ca Z .94 6' 0 '7.>5 17.,,
DO (mg/L) 8.1 8.0 8.a 3 °. -..71 (pimhos/c m A e3g~ 3cG I AlIka li n Ity \ \
Hardness ~ R3t<
11;, .. Temperature 1°C) 'Z5.3 Z:5.Z 2Zs 2. 1 25 2 5. 3 3t'
.i1 pH (S.U.) g. I B _ _ t+ qD 8.3 7,>
11 10.98% DO (mg/L) ( f s3 B. _:.Be1 ~
t ,
- I 11 , ,(gmhos/cm) RX,2t) Z2 5L 3 i, Temperature(°C) 25,3 Z5,Z. zc; z5.1 ZS5c0 Z.5.3 pH (S.U.) zfo00X It000t=__ wX7es 22% DO (mzlL) 8.3 . 1 7 Conductivity Z5j20 pH (S.U.) 17.0 -. B3. ItI , 15e( .0 I - qt 43.9% DO (mgJL) e3. 8 i _ .4F -. q i5. 1I ?t Conductivity 9Z?. (
I i (pmhos/cm) Zz-) Z '31Z53 Temperature (°C) ZS. 3.7 5. Z 5 1q ZS. z51.3
.pH (S.U.) _/- -7Af 6 .-ts _+.86q _ 3.a)l 7 100% DO (mefL) Z B .W i.s-( e Conductivity lqZ. z o*96
. (mernhos/cm) 28 1 -
.Temperature (°) -Z5 *Z 5 2S2 -zq*< Z53. I Zs.l
_pH (S.U.) 8. 1Z 63. CYT 8.i\9q 1 _- $1SJ _7qr Iq 100%Itk DO (mngtL) 8.-t _8-4 -3 to
.5 .9 !3 sl (grmhostcm) O95__
(mez CaCO31L) _12 .2_
(TR cghlorinAe A1: Cl, Temperature ('C) 25 3 Z-Z Z.9 IZ5. 1 vZS O ZS,a3 Condutivil F-a ntn i~ Int" F in
Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephalespromelas Date: _2po-465 _,4 Client: TVA I Sequoyah Nuclear Plant - Non-treated
Environmental Testing Solutions, Inc.
Chronic Whole Effuent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphliadubia Daily Chemical Analyses Client: Sequoyals Nuclear Plait Non-Trented Test dates: February 03 . 10,2004 Project number: 1042 Reviewed by:
l6w,m,,
Concentration Parameter Day a Day I I ay 2 Vay 3 Day 4 Day 5 Day 6 Initial Final Initial F'inal Inifinl I Final Initial IFinal Initial Final Initial Final Initial Final II12(SU) 8.0) 8.04 8.02 7.961 8.051 8.02 8.12 7.'8 8.11 8.0. 8.2 8 8.3 DO (mn/L) S.l 8.2 8.0 8.0 8.21 7.8 7.'. 8.21 7.7 7.6 8.2 7.8 8.2 Conducllvity (pnmhos/cn) 298 287 Control Aikaliniy (mgitL CaCOsi 61 61 Ilardness (mntL CaCO3 ) 84 84 Temperature (-C) 24.8 24.6 24.8 24.51 24.6 24.5 25.3 24.7 25.1 24.6 24.4 24.5 24.6 24.5 fil(SU) 8.13 8.07 8A11 7.92 8.03 8.02 8.0) 7.93 809 800 8.17 8.10 8.12 8.06 10.98% DO(mg/L) 8.3 8.3 8.3 8.0 8.i 7.8 8.0 8.2 7.7 8.1 8.1 8.2 8.2 Conductivity (Ipmhosmi) 278 l_ 279 302 282 270 294 281 Temperature (C) 24.9 24.6 24.8 24.5 24.6 24.5 25.3 24.7 25.1 24.6 24.5 24.5 24.6 24.5 nli (SU) 8.11 8.07 8.12 7.93 8.02 8.04 8.0 8.00 8.08 8.0 8.17 8.i2 8.13 8.07 22% DO (mr/L) 8.31 8.3 8.31 7.9 8.1 7. 8.0 8.2 83 7.7 8.1 8.2 8.3 8.3 Condnclivity (VmhorscmIl289 l 270 275 1278 263 2 262 Temperature (CC) 24.9 2 2426 248246 24.5 25.3 24.7 25.1 246 24.5 24.5 24.6 24.5 I1H (SUI) 8.11 8.08 8.1 795 8.0 8.05 8.27) 800 8.08 8.00 8.1 . 8.06 9DO (mn/L) 8.4 8.3 8.4 80 8.1 7.9 8.1 82 8.2 7.7 8. 63 8.4 8.4 4Condiclivity (pnmhoskm) 24') 256 265 241 256 243 240
_Teanperalure (-C) 24.91 24.6 24.8 24.5 246 24.5 25. 24.7 25.1 24.6 24.5 24.5 24.6 24.5 fll (SU) '8.III 8.22I 8.111 7.961 8.02 8.061 8.0') 8.02I 8.09I 8.08 8.14 8.13 8.M =8?. 2I 72%
DO (mg/L) 1 831 8.3 8.41 8.0[ 8.2! 7.9 6.01 8.01 8.3[ 7.71 - 8.31 8.3[ 8.41 8.4 he Bo S Vnnndrreivilv h,,1,ntmcn1 S & b E 220 _ 22 _ - -233 ~ __217
_^t 77V',
225 -I-A .
217 remseraftire IT) 24.9 24.81 2 4 24.05 24 C4.251 245,.3
- 4. zatr (*C)4.
nilt Nth2 4.-.-
X812 X11 8,111 X0 8.021 8 0', 8.07 K ^.
DOll lntn1.1
-: : .- X 4 7.91X 7.' ), ?}8 8.;
Conductivity (UrnlIosmnll) 97192 1' R8 1984 l}z) Allele) 100% Alkalinity (niglL CaCO3)
Ilarilness (tnevl, C'aCO,)
caonss(mn.4-OJ F'tal fresidual ilorlne - (mnr/l) t l <0.10 -_0.10 re,,mernltire CT)
I 249- < 24.8r -24 24.61 24.51 2 .31 4.7 4- . 4.
1121 (SU) 8.111 8.001 8.041 8.021 8.071 X.0)}
DO1(2 tnwfIA 8.2 8.5 8.1 7.8 7.8 8.2 S. 8.41 . 8.0 85 8.3 8.5 8.4X 4-Conductivity fumlhoscmn) ')4 197 --
l~t zl4-rl 100% Intake Alkalinity (mg/L CaCO,))
hardness (mi tL CaCO.) 804 4-rotat Itesidual Cl'lorlne fniIL)
C5,Co,)
Teninerature AIiltitilr TemlernhJre SAC) e 6 C'C)(misL A:-
w t X
- 24. 249 2.__2_62 _ 2.__ 51 ____4 z45 45 2.6_2.
Environmental Testing Solutions, Inc. Page 6 of 7 Species: Ceriodaphxniadu<bla Date: OZ02 3j.oq Client: Seauovah Nuclear Plant - Non-treated Dai* Chemistry:
Dav Analyst lAY Yy 17 dW4 Concentration Parameter wxl Tg:.it,;-,
zt>>
74777^ 'JA9, CO:NTROL RH (S.U.1 B .09 8p.0o -68. 07a 3 ob %0 G.o DO (mg/L) B.I uo 8 .o b2- -. 8 Conductivity 2 M.3 l 3 (mg CaCOVL) INR \ \ W~M Hardness .86l\4 Temperature ('C) 24 . R} sl LO Io Zi-) Z .5 atq .p zq..*S
. pH (S.U.) O-t 3 B _M 8B At -l -.q 91 a-6.z 10.98% DO (mgJL) R b3 S.3 o .6. I -7.S
(,umhos/cm') Z3 _+Ot =* _ _MI M Temperature (*C) Zt-q 2vi.(O zq16-6 2qS
-. 1 '2J-pH (S.U.) !5.11 6a-o7 9 Zt I . 8.0a7 8 -CO 22% DO (mg/L) e, :2 _+325
.9I 0, 1 7. Iq
(,umhoslcm) ZO27-,8 Temperature (°C) Z4-k _1 Z41.@O _Z4@. VXZq.5> Iq. _
pH (S.U.1 ^-t 8 ({I-i SQ55 . 8 o05 43.9% DO (mg/L) _0t 3 8 L4 13.0 -*1
- 7. q ConductivityrZ :5Zo Temperature (T) 2z q Z4l,L0 tq,6 _ 4.5 Z-1.(, Zq-5 PH(s.u.) 1 a1 8 it G-t 4.1q t q__ o1 72% DO (me/L) 113 2 r .O 0 Conductivity ZOZ3 g Z3 Temperature (°C) Wt 5 t b 21 I9 Z '1-5 Zl pH (S.U.) s tI ca <.
?-1 R 09 100% DO (mz/L) B.t .3 q.W )9 T9 -
Conductivitv s,3
{0d (mg CaCO/L) 1 VIlo (mg CaCO 1l.) ( - B T Rch10 Op r40 I tC:)\g9.1CJ.
F!O.
Temperature (°) 2Y el zY.(O z443 2z45 zqJ.> z -.5 pH (S.U.) 0 '}j 6 w15 8-tt _76 0.S 8o 100%M Intako DO (m/l.) _3 X z B5 a.1Xw,3 b Conductivity qOv°~ 9w.+*>jg (me CaCOJIL) 1 j ,\g E (s :
TmR chlorine .l :\ (.
Temperature (°C) 2.q.q Z~x .,5 ZY-S z.( ;Zq*5 Initial Final I Initial Final Final Initial Final laititl l l - - - - X - - - v v - - - w 5 l lnitill Flaal
Environmental Testing. Solutions, Inc. Page 7 of 7 Species: Ceriodaphlniaditbia Client: Senuovah Nsuclear Plant - N'on-treated Date: 0,2-- 3 .0 Dy.
4 _
Analyst 0*0-~ UA C¢Y Conctn- lParameter CO.NTROL pH (S.U.) 1.\ fit tX-0*Z DO (mg/L) 1 a e* .2 - 6.1 RB _8 filmhos/cm) 't 3: Z5 6 .
(mg CaCO3/L \. 0i toW \
(mge CaCO3/L.) 0\ICMa .' 'eY \ 8 Temperature (TC) Z6. 3 z4. :r5. 2q.to Z- S 7-.e Z4-ko zq.5s pH (S.U.) 8-0q '7.'1?3 6) 0q S.00 ax.W1 e>.10 8.12 80 10.98°/ DO (mg/L) . (38-
.Z . 3\31 .2
- 32 .
61imhoslcm)Z8 Z4 e3Z . -
Temperature(C lZS. 3 Z. I4S Z. i, 7~.11S to Z.
.pH (S.U.) B6oS 6 .CDO e .cB a0 6.11 0-11 G13 tO (gmhos/cm) Z k.o3 Z4 6 Y-M___r1_ Zl'1 2LoZ e.
_Temperature M°) Zg. 3 } ZQ Z51 2 2l 4itO-S4 ~ 22.5 -IS pH (S.U.) t O1 8.O 8 05
- aap 6.1 6.11 . _S.OL 43.9% DO (mfL.) _ . 32 8Z _ 02 .5 6A _.
Conductivity 'l rX25ZwZ Temperature (C) zs,3- li 24.4 ZS-1 2 -4.to '21.Si _A' Zo -I S
pH (S.U.) gl .8 0t3 I.1, 6OJF8.3 A-CA E37l7 100% DO (m&11.) B .z B 8 Le { 3 ez .
(umhos/cm) 'Z ote t^ 1 Is_q Z tep
.Temperature (°) . 35 zql.4 ZSJ Z. .5 ZlS Z. 2-pH (S.U.) 5 O1 2) 9 gj I 05 *09 30e &I, 6lS O-1
- 63. I (.a-i In0t0%~ DO (mg/L.) . . { I. , ,5 091
,3.7- B
.Alkalinity \ - w3 \
(me CaCO L)\t0 t TR chlorine (me/L) 4l .;..-,
- nLO<n+10.
............\Glf~.M ,
Temperature (°C) ZS .3 2q. ZS . t Z~LA 2-4. S Z4 -5 ZV.1,2e -
pH (S.U.) 5 C> -7 8.09 1l~i41 60 ,I -~ .1 0 Initial Final Initial Initial
- - . 11 Final
- 11 Final-- . Initial Final
Environmental Tcsting Solutions, Inc.
Chronic Whole Ernluent Toxicity Tcst (EPA-821-R-02-013, Mcthod 1000.0)
Species: Pintephaiespromnelas Daily Chemical Analyses Client: Sequoyala Nuclear Plant UV.Treated Test dates: February 03 .10, 2004 Project number: 1042 Reviewed 44 ,2/ .
Concentration Parameter Iay 0 D 1ay U D 2 Day 3 Day 4 l la S Day 6 Initi:il Flinal initial Initial FItunl Initial I lnal Inliial Finaln Initial l inal Initial Final pit (SU) 8.051 7.87 8.08 7.88 8.03 7.94 8.08 7.92 8.091 7.94 8.14 7.0 8.09 7.6 D)O (ingtL) 8.21 8.0 8.2 7.7 8.0 7.8 7.8 7.9 8.3 7.9 8.3 7.5 8.1 8.0 Conductivity (pmnhos/cm) 28 292 309 279 29 283 276 Control Alkalinity (mgfL CaCO 3)
Hardness (mg/L CaCOj)
Tempern-fo re (_C) 25.3 25.0 24.7 24.9 25.2 25.2 25.0 24.8 25.1 24.9 24.7 24.9 24.6 24.8 PII (SU) 8.05 7.88 8.10 7.85 8.04 7.91 8.0) 7.91 8.09 7.93 8.14 7.81 8.08 7.68 1 8 (mg/L)
DO 8.3 8.0 8.2 7.7 7.9 7.7 7.9 7.8 . 8.4 7.9 8.2 7.6 8.2 8.0 10.98% Conductivity (plmhos/cm) 2789 _27 _
Temperature (OC) 25.4 25.0 24.7 24.9 25.2 25.2 25.0 24.8 25.1 24.9 24.7 24.9 24.6 24.8 OttI (SU) 8.05 7.87 8.09 7.88 8.04 7.94 8.08 7.88 8.08 7.89 8.15 7.85 8.08 7.81 22% D)O (m. /L) 8.2 8.0 8.2 7.6 8.0 7.8 7.9 7.8 8.4 7.8 8.2 7.7 8.2 7.8 Conductivity (pminos/cm) 268 28C 289
_ 268 _27 268 _6
-remperature (-C) 25.4 2S.0 24.7 4.9 25.2 25.2 25.0 24.8 25.1 24.9 24.7 24.9 24.6 24.8 pit (SU) 8.04 7.90 8.09 7.91 8.03 7.93 8.08 7.93 8.08 7.90 8.14 7.84 8.0) 7.67 i)O (mg/L) 8.3 8.0 .2 7.7 8.1 7.8 7.9 7.8 8.3 7.8 8.2 7.3 8.2 X.l Conductivity (pmhoslcm) 246 254 262 245 254 244 241 TemrperaItire (C) 25.4 25.0 24.7 24.9 25.2 25.0 24.8 25.1 24.9 24.7 24.6 24.8 P11 (SU) 8.04 7.95 8.!! 7.93 8.03 7.91 8.07 7.96 8.09 7.96 8.13 7.86 8.05 7.79 72 DO (mngL)
D 8.2 8.0 8.2 7.7 8.1 7.8 7.9 7.8 8.3 7.8 8.2 7.8 8.1 8.1 Conductivity (pimhos/cm) 219 224 232 0 _ 2117 215 Temperature (C) 25.3 25.0 24.7 24.9 25.2 25.0 24.8 25.1 24.9 24.7 24.6 24.8 1lll (SU) 8.03 7.95 8.10 7.97 8.04 . 7.97 8.06 7.94 8.10 7.98 8.13 7.94 8.05 766 I)O (m1gli.) 8.l 7.9 8.2 7.6 8.1 7.9 8.0 7.8 8.4 7.9 8.3 7.8 8.2 8.1 in8 Conductivity (Pmimus/cm) 196 199 183 197 l96 I X 100% IAkalinity (mg/. CaCOj) lHardness (mg/L CaCO,)
iotal Residual Chlorine (m L)
________Tmperature (*C) 25.41 25.01 24.7 24.9 25.2 25.21 .25.01 24.8 25.11 24.91 24.71 24.9 24.6 2.
pI (SU) 8.041 7.961 8.091 7.98 8.05 7.981 8.051 7.951 8.121 7.941 8.11 7.90) 8.02 773 DO (mg/l.) 8.41 8.11 8.31 7.9 8.0 7.9 8.01 7.81 8.41 8.11 8.31 7.91 8.1 8.2 Conductivity C niiws/.cmu) 814171220201 100% Intake Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCOC)
Total Residual Chlorine (mg/L)
_________'remperafure CC) 1 25.41 25.01 24.71 24.91 25.21 25.21 25.01 24.8j 25.11 24.91 24.71 24.91 21.61 24.8j
Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pinteplhalesprornelas Date: Dt-_3.oq Client: TVA / Sequoyah Nuclear Plant - UV-treated
Environmental Testin, Solutions, Inc. Page 6 of 6 Species: Pimephxalespromelas Date: 0;2 -03 09 Client: TVA / Sequoyah Nuclear Plant - UV-treated Dav Concen- Paramelter - ..
CON'TROL -pH (S.U.) 0.6 '7-17 6.0,q 4.q 4 -,. 1L 150° B-Oq 1.
DO (mg/L) 7.4 - Bs3 w .3 +.15 ejl f0 ConductivityZ 7j2723ll21..
Alkzalinity i 4\0 (Hmard~nes~sOL V \ E Temperature (°C) 25,2_Z 2q.6 >. 2L .-4 Z4. o Z4( zld
_ _pH (S.U.) 6.0 -04 7. 9? ( B-f oq .3 +-.61 8-o6 -4 *o 10.98°% DO (mg/L) d5 7,5 8. -+
t1 3B2 -q. to . R
(;imhos/cm) "Z12O42 Z2 Temperature (°C) Z5 Z 5-? 1 Z. 24.- Z t.A 7Z. (,o Z L~3
_-pH (S.U.) _,O 1.03 0 . o8 6q 8 t. 5 686 e-06 .et 22% -DO (mglL) . 8. -4 ~ . . . ~
Cond uctivity ZQ5Z53'Zt v w Temperature (°C) ZS.O ZS Z5qI Zz4.9 7-.1 2t. -Zl. . Lo Y pH (S.U.) 130.Ob l.ga (IoB a .qc) 0,14 E34 13-s0q9 -:
43.9°/ -DO (metL) -7.1 d ELx 2Z 173 02 -
(,umhoslcm) Z~l ~f : Z14 I::-
Temperature (°C) zS.O .2.0. ZS. l Z1 M~-i Z2 A ZJA.(o Z0
-pH(S.U.) S.0 O 1.o1(
7eS 4.cle E.15 -+.8w 18o 14 7'% -DO (me/L) l, w3 e38.3 4.2 13 Bl6.1 Con du ctivity .. ZI -7 3 ... ..1.: :*. .*-.
Temperature (°) 5) Zl8 ; . i 4 z I.-4 7t A Z 00 S pH (S.U.) 5.0D -1.q S . f(D 4.9B A.1 : +k LPq I3OD Sto 100% -DO (mgJL) 8 .5 : X ',12(Z, t .
Conductivity 3;- I7 [p..,- l1 (mg CaCO.J) \ \ \;-.:\
Hardness .: \* ; \
TRChlorine (mglL) .X.:,..w .. ^.: 5 : :
Temperature (°C) z5.0 te 5 Z4.9C Z-ql.4 Vn Ze 4 nly PSU.) a. *,z' 100°/ DO (mg/L ' 8.o al.qq e4s ~
Intake ConductivityvJ8Zn i o<~..:
(mag CaCO)/L) \' i,.\ l;;'\-
Hardness\. ,.2 o ' *
TR chlorine (me/L) '4ll -:::.i;.T Temnernture rocM BSS.1 .y4.q Z LI -I 2 4.0
. -, - -__ . 1. - - . .. 1.- .. .- - -- - . 'r-_!Final .I I Fln1 T Int,- I Fi1n'l I nititl Finnl Initial T_'t:nl
--. a f . a . I .....- 1 .
Page .I Environmental Testing Solutions, Inc. Page ( of -is' Alkalinity (EPA Method 310.1)
Matrix: Water, MDL 1.0 mg CaCO 3/L Analyst Date analyzed - Z- fQ 0f Titrate samples to pH = 4.50 S.U.
Titrant normality and multiplierdetermination:
pH of Normality Normality(N) of H2SO4 pH Factor or Multiplier Delonized Tltrant check Bcgin End Total (5 ml Na2COJ) xO.OS)/E (hx (I 50000)/ 100 ml sample water reference standard ml ml inl -0.251E -Nx 500
- 4'5 S.U. number number (E) (acceptabic range - 0.018 - 0.022)
I&MRII Q1ZlIz ItAP b__ O_ Z- l2-5 _. _ _ _ _ __zo_ _ _ _
Laboratory control standard:
Reference True value Sample Alkalinity (MV) %RS IVITV x 100 standard number (TV) volume Begin End Total -Multiplier (mg CaCO,/L) (acceptable ranve (mg CaCOlL) (ml) ml ml ml -90to li0o.)
(e/F- ' 100 ,0 zooi q4 0 '.
Duplicatesam Ze Sample number i fSample precision:
Sample ID volume (ml)
Begin ml End ml Total ml Multiplier Alkalinity (mg CaCO)/L)
%RPD J(S. D)lI(S+DY2l1) 1I00 (acceptable range-* 10%)
- 02. D2,O l InH5 0102I J Duplicate ID loo Zz.4 Z13 .o 4 R lO10 4 [
S o D l o(t-
.Ov.4;;.; ....s, Matrirx spike recovepy:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CsCO9L)
(mg CACOVL) (ml) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R - MV ISV x 100 (mg CaCO,/L) MV- A - B (acceptable ranoe l (mg C2CG)/L) 7S 125%T
-7to 60 I 00 Samplc Ineasurentents:
Sample volume Begin End Total Alkalinity Sample number Sample ID (Ml) lnl lml ml Multiplier (mg C2COIL) oz.oz2-o4 SM,-sL 1*-'0 A 1c3 4s__ 6., I -o4 _ _ _.
IC)2 tW-0q l 6 Ut-o- -L A 4e-.s Ad&t1~ 3-3 1 i13
______0. __ :-37 ~
02-64 -4
_ _ _ _ _ _ __, m 14
- 66. s1-1.
I4j-0 t _ _ _ _ lw 17to t3 5.4 _s= 7 Z -_ _ r 14 WH X-50 _ I lq12 0 .( I -Q I 1.4
_O _ _ _ _
OZCA-Ovoq ss u-) O _ __ _ -o .A 1 !
p 4DzO2.oq I4SQII 1 1.2 l2-Z q.92 4 _ _ _ _ .b _ _ _
Reviewed by: De reviewed: I 02 O>§-oqz Date __
Page 2 Environmental Testing Solutions, Inc. Page 'T orf s Alkalinity (EPA Method 310.1)
Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst 91iK Date analyzed l ?.O LJ Titrate samples to pH = 4.50 S.U.
T itrannr=jazm ,.
pH of Normsality _1 _~ HmSO' pH Factor or Multiplier Delonized Titrant check Begin End Total =(5 ml NnCO3 x 0. - (NX 500001Y 100 ml stmpIc water reterence standard ml ml nil - 0.25/E w4.5 S.U. nnumber number (E- (accepfabte range - 0.01 S -C.022) __ _ _..
Laboratorycontrolstandard.: -
Reterence True value j Sample Alkalinity (.MV) %RS-MVITVx 100 standard number (TV) volume Begin End Total MultiplierI (Mg C&COIL) (acceptabkt range I I (mg CaCO/L) (ml) I ml I ml ml I I r90 to 110%) 1:.
156 01 0 0 k.1 5 I5.J 1 010.0I 0 1 Duplicatesam te precision:
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (msCaCOAL) J(S.D)/i(S+DY21 1oo00 number _ (ml) ml ml ml (accceptble range - k10%)
lt )OoI.0 2sl 155I. ao " ... .
cl
- Dplica_ 4 2-,0 -1_I _7_ _ D_ _,s Sample nieasurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (mI) ml nil ml Multiplier (mL,CaCOL) qLf)20JQ.02 6cQ& Nyr 3 CD UZ.8 4( .l7 1o. 0 73 b4o24q. 10 Gc0.? 1o I Z _ D3 'l 0 Ln l 7 04oto.o0 I 3 ____ 4. -7.5 D4tzo3.c1 GAunv -L I _ 4_3 li.z 1 6.4 i3
- o. 17 1 n 1Vo - -
040LI0-o.03 4 .,, .Ia ZI oS-5 - - A bq12C&O1 1- S'1 26.6 17-n17 3 S Z.
0DoDZL.c 1 Z_
Z l.1 3 532 :5.7 tAO2ol.o' 3 _______ 3-Reviewed by: l Date DYte reviewed:
rv l Q qS
Page Z..
Environmental Testing Solutions, Inc. Page of of_
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL - 1.0 mg CaCOI/L Analyst OM7 7 Dateanalyzed C) oe+
Laboratorycontrol standard: i' Reference True value Sample Hardness (MV) h R - MV/TV X 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO/fL) (acceptable range (m4 CaCO 090 ) ml ml ml 20 to IIo Duplicate sample precision:
Sample Hardness %RPD -
Sample Sample ED volume Begin End Total Multiplier (mg CsCOIL) J(S-D)Il(S+D)r2J z I00 number (ml) mr ml ml (acceptable range
- 10%)
V Duplicate V l f D .'
Matrix spike reco ___
Reterence Spike value Sample Spike bMrdless (A) standard number (SIO volume Begin End Total 4ultiplier (mg CaCOj/L)
(ml! C2CO/} (MI) -ml ml m~l IM Itob bD 6f ;2t.< 21.o0 40 2°c0A Sample hardness (B) Measured spike value (NIV) % R - MVISV x 100 (mg CaCO.L) . AV - A - B (acceptable range l (mg CaCO,1L) _75 to 125%)
8 13 4 to_ -
Sample nteasurementss:_
Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CnCO9L)
- Blank
_____(shouldbe-OmngCaCO/) 0o 0.0 O-0 ZO.-14 ______
t:z.
Mc>zL.O . -Mgt,- 420 i; T
.~~2c .I Z ~.i~~ . L-St ._._._.
4.
4-t 1 I B4
%6th-0oAA_ 45 OZ-o'4*c WWL j0o 35.a3 .4 4.1 __
I ____12 to 34 1.4L 2-t. _4 l o4 1'W*4 l-b - - 5 4.( l_ _ _4 v,1o~O~f~~ ___ 3 q15' I+0Lc0.19 4 2.4 -I-- I Note: If >1ISml of tirant is used. samplc mustbc diluted. Reviewed IL i i i l Date reviewed I C>2 D by:
Page 3 Environmental Testing Solutions, Inc. Page ZL of Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCOI/L Analyst [9III l7 Dale analyzed l Titrant norlmaly and multipier determur iii Titrant Normality check Begin End Total tv A) or EDTA pH Factor or Multipier reference standard ml ml ml 0. _ (Ns 50000)/SO ml sample number number =_ = (acceptablerange-0.01S -0.022) -NX 1000 Laboratorycontrolstandard:
1.
. Reference True value Sample % RS -MVITV 100 J
Hardness (;MV) standard number (TV) volume Begin End Total Multiplier (Mg C2COA.) (occeptable range (mg CaCOAtL) (mD M ml ml . 90 to 110%)
IkS5lo 40 507i5 %3.4 Zo 2c4 IOZ.5%
Duplicatesampl precision:
Sample Hardness %RPD Sumple Sample ID volume Begin End Total Multiplier (mg CrCO/L).
_(ScepD)ale(S+Dy2e) x 100 Sumber (m e ml ml ml (MeCeptable range t10%)
OL4020.o IA at _ 4t zo - 1q .(
_ __Duplicate Duliat 4 s ~ 4. z. D 24 4c-&~
Matrixspike recove j: S wM l Reference standard number J Spike(SV)value implel volume Begin End Total Multiplier f Spike hardness (A)
(mgCaCOIL)
(mc CaCO31L) (ml) msl I n ml lml (kiCSL so SO 14oZ.5.i l 1. 2D-4 1 12 l Sample hardness (B) Measured spike value (NIM l R MV SV x 100l (mg CaCO,/L) NWIV - A - B (acceptable range (mg CaCO,/L) -75 to 125%) l
- Sample mneasuremenets: _______
Sample volume Begin End Total Hardness Sample number Sample ID (ml) nml ml nml Multiplier (m: CaCO,/L) 04_I07 c e l _ Z5 1l in 4 I I l>0q20(p.0)
OqL()Zjca.lq l4t12 :5- As I 34___
134 (-l l l1 58 -7 014C=V5o.O1 5a 61 3 1e 213ol1-4-T_ 1 84
{o~b 20 cS .o g _Z__ ___ 11 f
_____________ i 0CLCZ-14S~~oMe 0 2 05- I Z 6\-
l 48 ?3 I i- 8 1C)VOZo tv3> ivzsf 001I l4.q 1q -'I 1 1 4 O D7rDZ S05 T__o_ r_ 1 _z ISO Note: If >t5ml ortitmint is uscd. sample must be diluted. Reviewed I V, -] 1- Date reviewed I C>2 OgC) __jj by:
Environmental Testing Solutions, Inc. -52ns1.
Page 16 Page I of Z Total Residual Chlorine (EPA Method 330.5)
Matrix: Water,MDL=0.10rngfL Meter Accurnet Model AR25 pWlon Meter Analyst hE 7 I 7] Iodide reagent: INA 117 Date analyzed l_023 q Acid reagent: f I , 1I 1 Calibration:
I - 0.0omelL I 1.00Oim/L Reference standard number I I 61 lt4I 1 5,5, -
Note: For samples with a residual chlorine of > 1.0 mgrL. the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrolstandard.:
Reference standard True value M) Measured value OfV) % RS - MV/ TV X100 number (mg/L) I (mgrL) I (aceeptablerange-90to 110%)
j-tS5 q 0.50 O.'-4 5 I' Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mngL)
.-. . :.-Blank (shouldbei<O.10rnmL) LA (SqwZ! .r Lm,7 in+iv-% LA)u P llW _O.OOOA13 nckz06. ICJ MornroeQ WLvrfP nocf4oer-. CtO 40.eO 1 q3 CA f f PC5
. 1kj . OD -q nibido C100-et, < .0003,74-045. i(3cjwrcu Wa it 4.OOO103o
_ _ _ _ __C5 _ _5 _ _ _ _ _ _ _ _ b OO Ot l z 6 .01 ai1iL O nrdL 4.o4oz 64ZG3.0t-.1 paOI+ - ct4 _0.0 Arote' Al! samples were analyzed in excess or EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control raaad Reference standard True value (TV) Itreasured value (MV) % RS - MV ITVr 100 number (mL) . (acceptable r -ne m90 to 110%)
I0.50 -1 Reviewed by Date reviewed l > L- A.
Environmental Testing Solutions, Inc.
Page I I page 2- of z Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mglL Meler: Accumet Model AR25 pH/Ion Meter Analyst l KY Iodide reagent: l 7 Date analyzed l02I,0 . 3 Acid reagent: lSI Calibration:
. .: . 0.10 mgL 1.00 mz/L l Reterence standard number Notw: For samples with a residual chlorine or > 1.0 mglL the calibration range must be adjusted to bracket the chlorine levels of thc saples.
Laboratorycontrolstandard: _
Reeren e standard 0 True value (TV) Measured value (MV) *%RS - MV ITVx 100 number (m L) I(mglL) (acceptable range - 90 to 110%)
1055153q 0.S0 1 -qe)(P 9I I Sampil measurements:
Sample Sample ID Sample characteristics Residual chlorine number (melL)
-s: .
- Blank (should be - <0.10 mg/L) ,
- a<0nabC (.4t
&< r d-0' CreJ no ca cu~t CLfr A.-30 6 2w3:.acs3 T,) ie n(o C.O=4~, cut. 40.
LooL t O4eRzc2-09 I r' o Ci..O L.OOot1*10 Hare: All satmples wee anaulyzed inexcess ot EPSA recommrended holding time (IS m~inutes) unless otherwise noted.
Reviewed by Date reviewed - - I
Environmental Testing Solutions, [nc. : 9 Page .. j 4 Page 1 of I I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 Ing/L II Analyst I !:
Meter: Accurnet Model AR25 pH/Ion Meter Iodide reagent lI PhiI IZIJ Date analyzed T-0! 7 Acid reagent: 'w e- to,2 Calibration:
I I. ,
Reterence standard number It Note: For samples with a residual chlorine of 0.10 mZIL I I
1.00 rn L
$t5 1S5 13 i
I 1.0 mpgL. the calibration range must be adjusted to bracket the chlorine levels of the samples.
I Laboratorycontrolstandard:
jT155 Reference standard number,
/0.50 True value (TV)
(mg!L)
'f I
I.
Measured value (MV)
(mg/L)
% RS - MVITVx 100 (acceptable range -90 to 110%)
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD - I(S - D) /l(S+D)1211 s 100 number _____ _ (acceptable range- d 10%)
- 1Duplicate D/( o Sample measurements:
Sample Sample [D Sample characteristics Residual chlorine number (melL)
K.: . .* Blank Lshouldbc-<0.10mgIL) v.', -.
1-~s~
3 _ _ _ _ _ _ _ . _ OQCAt o Ops' r _ _ _ _ _ _ _~
ZI< r-, D4S6 hb V-ti !4(o2C5.bZ Y a'-A0 tD J-1 ck < O bO0I?12-,
No~oze.z oqozc ra nV e L
.cA ; lflC/VLtZ4A _______
I bq QU.b DU
_______ (lc~1/2 r,,
/-DuMYoZtf 1o-Jp S' . a-bol o,-
b)407105. 0V 4c1( <.Ocl LAO21S. IV U 04b eA l; p v.000031 I
.'e Note: All samples were anolyzed in excess otEPA recommnended holding dinre (t5minutes) unlcss otherwise noted.
Laboratory control standard:
i Rercrence standard Inumber /
True value (TV)
(m:IL)
Measured value (hIV)
(mg[L)
% RSUD V ITV s I00 (Aceeptable rnnwr - 90 to I lO%)
0.50 .523 I Reviewed by - 1*
Date reviewed l Oq:))4Q l I
I, Environmental Testing Solutions, Inc. -50 Z 'o Page Jt5 Page I..Lo~
11 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL 0. OmgfL Meter Accumet Model AR25 pH/Ion Meter Analyst F6* 47 lodide reagent: [jp- l ,
Date analyzed l OZ.i°4 Acidreagent: likip- lI a I
Calibration:
- -- 'l 0.10 Ing/L I 1.00 mg/ _
I Reference standard number I lSS 1341 I I NS5 i L(
Note: Forsampleswich a residual chlorine of>1.0 mgIL. the calibratlon range must be adjusted to bracket the chlorine levels ofthe samples.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) %RS-MVlTVx l00 number (rimgL)
(mgIL) (acceptable rnge - 90 to I tO%)
jIJsS i3 t- 0.50 6-45 3 --
Duplicatesam4eprecislon:
Sample Sample ID Sample characteristics Residuaklchlorine %RPD - {(S - D)/I(S+D)121) X100 number mrngmL) (acceptable range 10%)
_ _ _ _ S- S ~c e"oo ;q Duplicate : DC Sample measurements:.
Sample Sample MD Sample characterlstics Residual chlorine number (mgAL)
Blank (shouldbe- D 00m/)g jq ." .D-. . '. ; , s7.- .. ___________
.,A 6A -41 <000445o.oooboqO o4ow;-Lo IS 1 AkjR.o1 Pa LULAA'- 0 7-g5 0',o2O( L c rohfli , pU1 Co .Z oo4
'467fl 1~3 f&torkboro rL lQJ pOA3 LZ2 Oq aZO7.O1 F ckLLS 0.o01 O Note: All samples were analyzed inexcess orEPA recommended holding time (15 minutes) unless ot rwise noted.
Reference standard True value (TV) M tu) e%RS - ;IV/TV x 100 number (mgIL) (mglL) blerange-90toll0%)
0.50 Reviewed by -
Date reviewed
Environmental Testing Solutions, Inc.
Page L 1 Page . of .3 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL - 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst l 7 ] Iodide reagent:
Date analyzed l ozi7.of I Acid reagent: l Z_1
... . O.IlQJ mg/L0 Reference standard number I Note: For samples With a residual chlorine of > 1.0 mg/l, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrolstandard:
Reference standard True value (TV) Measured value (MV) % RS -NMV I TV x 100 number (mg/L) (mglL) (acceptable range - 90 to I tO%)
MSS 1S 0.50 4q7 ..
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number . . (meJL)
Blank (shouldbe-< .tOmg!L) . ^ -.. Ž:k: a*S;;;'
4oI20CV.oA - tc L-> P2 . Qc o A\- ,fpQ'cL <O.OO7S o4Dloc.. DI SZ J - I nA I It .O <Ip0 00c4
. ()-:p / a -M Note: All samples were analyzed in excess of EPA recomnmended holding time (15 minutes) unless otherwise noted.
Laboratorycontrol standard:-
Reference standard True value (TV) Measured value (MV) % RS -MV I TVxI 100 number I (mZfL) (mg-IL) (aceeptablernge-90 tollD%)
. 34 0.50 Xn 0. q4 To Rcviewcd by I 1 . 2 Date reviewed I ]
Sequoyah Nuclear Plant Biomonitoring February 3-10, 2004 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimnephalespromielas using Moderately Hard Synthetic Water 1.0 L j T i rI I I II I IIII 0.9 K State and USEPA MandatedControlLimits Limits SetAccording to 4 2 StandardDeviations 0.8 0.7 ....... . ............... ...................................................................................
0.6 4 0.5 ..........................................................................................................................
t 0.4
- e 1.0 U
0.9 USEPA Warning and ControlLimits Limits Set According to 1 0 hand 2 5th Percentile CVs N 0.8 0.7 -...................................................... ........................................................
0.6 ._
0.5 0.4
%AlS5 032Sgo.0iv, 30V°O" °36 O O 05V OV Z& -to, Test date 7-day IC.5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
- - Central Tendency (mean IC25)
Warning Limits (mean IC25 4 SA.10 )
.Control Limits (mean IC25 ESA.25 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Rererence Toxicant Control Chart for Pimephafespromelas using Moderately Hard Synthetic Water State wAnUSEPA Laboratory Laboratory USEPA USEPA Test munber Test date 7-daylCs CT S Control Limits S', Waffdnglimits SA25 Cosildrl its Smf Warning Undis Slue Centru Lindt CV (WLTCCI) (wLl-CI CT-2S CTt2S CT-S.,, Cr+S.,, 3 Cr-SA., CTrSAA. CT-SA, cAr 9A.i CT-SA., Cr+S,,
1 03-18-03 0.64 2 0448-03 0.50 0.57 0.10 037 0.76 0.07 0.50 0.63 0.12 0.45 0.69 0.22 0.35 0.78 0.26 0.31 0.32 0.17 3 04-15-03 056 0.57 0.07 0.43 0.70 0.07 0.50 0.63 0.12 0.45 0.68 0.21 0.35 0.78 0.25 0.31 0.82 0.12 4 04.29.03 0.59 0.57 0.06 0.46 0.69 0.07 0.50 0.64 0.12 0.45 0.69 0.22 0.35 0.79 0.26 0.31 0.83 0.10 5 05-29-03 0.64 0.59 0.06 0.47 0.70 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.10 6 06-17-03 0.60 0.59 0.05 0.48 0.69 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.Q1 0.26 0.32 0.85 0.09 1 07.15-03 0.65 0.60 0.05 0.49 0.70 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.Q2 0.27 0.33 0.87 0.09 a 0805-03 0.53 0.59 0.05 0.48 0.70 0.07 0.52 0.66 0.12 0.47 0.71 0.22 0.37 0.Q1 0.27 0.32 0.t5 0.09 9 0S-12-03 0.61 0.60 0.06 0.48 0.72 0.07 0.53 0.67 0.13 0.47 0.72 0.23 0.37 0.83 0.27 033 0.87 0.10 10 0t-19-03 0.55 0.59 0.06 0.48 0.71 0.07 0.52 0.67 0.1.2 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.86 0.10 11 104703 0.61 0.60 0.05 0.49 0.71 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.Q2 0.27 0.33 0.86 0.09 12 10-21.03 0.54 0.59 0.05 0.48 0.70 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.37 0.82 0.27 0.33 0.86 0.09 13 1144403 0.63 0.59 0.05 0.49 0.70 0.07 0.52 0.67 0.12 0.47 0.72 0.23 037 0.S2 0.27 0.33 0.86 0.09 14 11-11-03 0.53 0.59 0.05 0.48 0.70 0.07 0.52 0.66 O.2 0.47 0.71 0.22 0.37 0.81 0.27 0.32 0.86 0.09 15 11-11-03 0.3 0.59 0.05 0.48 0.69 0.07 0.52 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.09 16 1249-03 0.51 0.58 0.05 0.48 0.69 0.07 0.51 0.66 0.12 0.46 0.71 0.22 0.36 0.81 0.26 0.32 0.85 0.09 11 01-06-04 0.66 0.59 0.05 0.48 0.70 0.07 0.52 0.66 0.12 0.47 0.71 0.22 037 0.81 0.27 0.32 0.85 0.09 I1 01-13-04 0.64 0.59 0.05 049 0.70 0.07 0.52 0.66 0.12 0.47 0.72 0.22 0.37 0.82 0.27 033 0.86 0.09 19 01-2004 0.68 0.60 0.06 0.49 0.71 0.07 0.52 0.67 0.13 0.47 0.72 0.23 0.37 0.82 0.27 0.33 0.87 0.09 20 02-03404 0.66 0.60 0.06 0.49 0.71 0.07 0.53 0.67 0.13 0.47 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 Note: 7.4 IC y 25% kihOXib accatrataon. An ettim offthe oetactrtflt @rpobtm chbrW d&t woul cause a25% itductbn i Phnephaks gmwd for the test poptimn.
CT - OCrl tendency (meamr,).
S - Standad deiatian ftlhe M, vahes.
Laboratory Central aMd Waning LUndts Laboatoyc eooa damhig ks were estabshedhngdie .tdarddeiat3onorthe rC vlues copondigto the 10th uAd 25thpeiti CVc. Therelgesaremnore itient thatthe cmtoland wnh glib reco ended byUSEPA1br the teat method ad endpoist.
SA - Stndard dcvat conaespmdig to the 10 penestWe CV. (SA.1, - 0.12)
S,, = Standard devhtion coatsondig to the 25e pemmfile CV. (SA2 0.2 1)
USEPA C ntrel *nd Waning Unfts S.is - Standard dev aiw vereespndkigto the 75' peenmtle CV. ( Sn -03t)
SA.- Standard denstim coempondhgto de 90 perttLe CV. (SA Q0.45)
CV - Coefatient of vasxbn ofthe iC-2 Values.
RJ!EMA. tndhgt A hg fe Method t3hoR.
Vibly IWbOk Efbentibakify Awtiettk h tbdeeteSns I olbot Dtqdinee ElA tt . c y*Cll. 0lt O'ga~l..u &I./ Aquartic Be~t..Ie0.3 02-M40
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromtelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 _ ____- - _- __ _ _ __ __ _
0.7 .............. ..............
0.6
(.4 U . ._ .. .. ... ... ... ... _I. ... ... .
0.5
~~~~................
- -e _ - -
0.4 0.3 0.2 Test date 7-day IC25= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
- - Central Tendency (mean IC25)
Laboratory Warning Limits (mean IC2. + SA.I 0 . SAJO= 0.12)
.......... Control .Laboratory Limits (mean IC2 4 SA , SA25 = 0.21)
- - USEPA Warning Limits (mean IC25 : SA75, SA.75 =0.38)
USEPA Control Limits (mean IC25 + S.90, SA90 = 0.45)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toodcant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean CT SurvIval Growth cr CV MSD PMSD CT
(%) (mng/arvae) for Control Growth (%) for Control
('/.) for PMSD (Yo)
(%) (vac) (mg/1avae) Growth CV (%)
1 03-18-03 100 0.803 21.3 0.21 26.5 2 04-08-03 100 1.083, 0.943 6.1 13.7 0.09 8.0 17.2 3 04-15-03 100 0.892 0.926 17.0 14.8 0.17 18.7 17.7 4 04-29-03 97.5 1.021 0.950 6.7 12.8 0.18 17.5 17.7 5 05-29-03 100 1.005 0.961 7.9 11.8 0.11 11.1 16.4 6 06-17-03 97.5 0.888 0.949 4.2 10.6 0.18 20.7 17.1 7 07-15-03 100 0.910 0.943 6.7 10.0 0.12 13.7 16.6 8 08-05-03 97.5 1.092 0.962 6.5 9.6 0.13 11.8 16.0 9 08-12-03 100 0.693 0.932 9.7 9.6 0.20 28.2 17.4 10 08-19-03 100 0.745 0.913 2.9 8.9 0.08 10.7 16.7 11 10-07-03 97.5 0.762 0.899 20.0 9.9 0.18 23.1 17.3 12 10-21-03 100 0.803 0.891 5.7 9.6 0.13 16.4 17.2 13 11-04-03 100 0.895 0.892 7.4 9.4 0.10 11.6 16.8 14 11-11-03 100 0.895 0.892 7.5 9.3 0.12 13.6 16.5 15 11-11-03 100 0.953 0.896 11.6 9.4 0.20 21.3 16.9 16 12-09-03 100 0.744 0.886 2.2 9.0 0.18 24.2 17.3 17 01-06-04 97.5 0.816 0.882 4.6 8.7 0.13 15.5 17.2 18 01-13.04 100 0.807 0.878 3.3 8.4 0.10 12.5 17.0 19 01-20-04 100 0.770 0.872 16.5 8.8 0.13 17.0 17.0 20 02-03-04 100 0.847 0.871 5.2 8.6 0.11 13.0 16.8 Noite CV- coefficient or varation for control growth On average, the CV for control growth is 8.6% in Environmental Testing Solutions, Inc. Pimephalea chronic toxicity tests.
Lower CV bound detennined by USEPA (10i pereentile) - 3.5O.
tUpper CV bound determined by USEPA (90 percrentile) 20%
MSD - Minimum Signifcant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure oftestprecision The PMSD is Oe minimum pescent difference betwoen the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Envirorrnental Testing Solutiorn, Inc. chronic toxicity tests when a toxicant reduces Pimephaaer growthby 16.8% from the control LowerPMSD bound determinedbyUSEPA(10 percenhle) - 9.4%.
Upper PMSD bound determined by USEPA (90' percentile) - 35%.
CT - Central Terdincy (mean Control Growth, CV, or PMSD)
Ttu lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 labortories for Pimephales growth in chronic referene toxicant testh USEPA 2000. Undentanding ard Aceouling forMethod Vribility inWhole Efluent ToxicityApplications Under the Naonal PoUutantDischarge Eliminaton Progrm EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Organfsrnsoblainedfrom Aquaic BloSystems, Inc. 02-03-04
Environmental Testing Solutions, Inc.
PimephalespromelasControl Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1.50 : I I I I I I I, I I I 1 1 1 1 1 I I 1 1 1I 1.25 -
- 15 1.00 0.75 . ~................................
'U . ........ ..... ...........------ ........ ........ ................
0.50 0.25 Minimum Acceptance Criteria (0.25 mg per surviving larvae)
-~~~~ I ~I ~I I I I
- - - 1 I I I I I I I I I I I I I I I I I I -
40 North Carolina Acceptance Limit (< 40.0%)
1-1
§* 30 1._
Kentucky Acceptance Limit (< 30.0%)
- . ' 2 C3 2 ajCQ z 10 o 0 Test date
- Control Growth or Coefficient of Variation (CV)
- - Central Tendency (mean Control Growth or CV)
.. Control Limits (mean Control Growth or CV 4 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimnephales promnelas using Moderately Hard Synthetic Water 40 _
30 01-UP 20 10 \ / ...................................... ,............... 7...............=...............\.......
I I I I I I I I I I I I 0 I I I I I I I I 0Y.'so te, oo 0) -")", oo zso -o 'Tes da°'te°t Test date
- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- - Central Tendency (mean PMSD) and Upper PMSD .Lower
.---- Bounds Lower PMSD Bound (lOh percentile) 9.4%, Upper PMSD Bound (9O0" percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Environmental Testing Solutions, Inc. Page I of 5 Jim Sumner I attest to the accuracy and Integrity Potassium Chloride Chronic Reference Toxicant Test of this document (EPA-821-R-02-013 Method 1000.0) 2004.02.14 17:07:05 .0500 Species: Pimephalespromelas PpKCICR Test Number _gk Dilution preparationinformation: Comments:
KCI CHM number: CUi o&Tl Stock preparation: 50 g KCI4L Dissolve SOg KCI in I-L Deionized water Dilution prep (mgtL) 300 450 600 750 900 Stockvolume (l.) 6 9 12 15 18 Diluent volume (mL) 994 991 98S 9S5 982 Total volumc (ml.) 1000 1000 1000 1000 1000 Test organism information: - Test information:
Organism age: 22VS -co Z tb\).V%? Otb Randomizing template: eLe Date and times organisms o-ozcq-U %COA -'-n l RSr Incubator number and were born between: shelf location:
Organism source: A6& b6 R 6o- zcz.-o4 Arlemla lot number B663o0Q Transferbowl information: pH SU Temperature- 'C. Total drying time: ______R5
.p .S.2- Date /Time in: a40z j53..
Average transfer volume: _ Date I Time out:Dt.ll.b 153C
. __ __ __ _ Oven temperature: C Dalyfeeding and renewalinformation:
Day Date Morning Afternoon Test Initiation, Control water Analyst feeding feeding time renewal, or batch used time termination time O 02O.5 -*f -$ j NnrOZO.2-0 (I7c0 - 9 I ox-o)'.c4
- qSi 0Losz 136t 'a-2-0c-Z. ___
2 !)Z-O_-' tq4Jp I jltI 3 OZ1b0.cZ 4 _
3 oz.Obo.0q OqcS 1_IO OZ-O2-Oq VeA-4 Oz-o1-oq I 02o (91o 10 o;2-C>-D4 _
-5 : 7 2 , 0 9 ol4CI l'4oC)
L1OC O- cUc ____>_
- 7 0 --,1-04J 9tASz.@.^.
-D Z I7s I> A-jptp4 1340
{O aD<2;ow=L;.','2 Control information: Acceptance criteria Summarr of test endpoints:
% Mortality. 0C 20% 7-day LC5 17 32 . 8 Average weight per initial larvae: I0.
DI1 Sq : - ; NOEC I Average weight per surviving larvae: 0.fj1 _ o025 mg'larvac LOEC 7{5 IChV IC251C ~'
Environmental Testing Solutions. Inc. Page 2 of S Environmental TestinE Solutions, Inc. Page2afS Species: Pimephalespromelas PpKCICR Test Number: 7 Survival and Growth Data
.Day CONTROL - 300 m KUIL 450 mg KCVIL A B C D Ej F G H l J K L 0 /0 1, ID0 0 /0 to /0 Io /0 /0 (0 /D 10 10 (0 /0 to /( /0 /0 /0 /0 /0 (O
//0( /0 /0 ID /0 /0 /0 10 9 IC Io 3 c o0I 0 D IC '0,0 q Ioii 10)
_ __ to IC) Io 10 0 I_C 10 9 10 10
_ _10 ID0 10 10 /0 10 /( 10 10 t/o 10 6
ID /0 / 0/0 0/0// 0/o 10 I C) 7 10 LA 1 10 10 to 1( 10 10 9 o It A- Pan weight (mg) -4' -b _.o_
B - Pan + Larvae weight _ _ _
(Me) "P o. _oil '\ ' '1- @ _
C- Larvae weight (mg) , ~'
-A-B9 O
_ _ _ _ _ _ %,r **
6*~ ,
6 Weight per MUMa number ofo p, A 44' NV
- C I Ilotial number of laryae 0O*I
- OC O O D' O' O- CY O'. .O Average weight per Initil Qql number of larvae (mg) 0. 0. 79 0. £31
% t: I..,7v, o27 ger% I.qT7, Calculations and data reviewed: @_
Comments:
I Environmental Testing Solutions, Inc. Page 3 of 5 Pg~(
I II Species: Pimephalespromelas PpKCICR Test Number Vo I
I lDay I Da 600'i Comments:
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821JR-024013, Method 1000.0)
Species: Pimephalspromelas Quality Control Veriflcatlon of Data Entry, Calculations, and Statistical Analyses Tonssuee PP8KCIM (NIS(At
.33 Depot St.)
Tat duket: Ftbr73-10.2004 Revetwedub cA__ si A.r(V -t+ dm M-VgWS Cdtkk i *_h W n-s _-__ £66 ii_ Coiiwi 4 _ ,_a 3 V'O"' -0 A I30 I30 14803 2.3.37 6.567 0.337 0.657 Com a _ I_ _u 31 s 35.047 2o32 e .203 0*"20 0.147 3.2 0870 3*** 0.947 52 Retapptleable to0 30 1494 23.94 9.036 O..7. 0.904 D to lo 14.462 22.92 8.058 0026 0.06
- to t 14926 23.41 1.544 0so54 0654 3 _0 t10 10 14.786 2?76 7.97 0.794 71 0 797 O_ 30 0 o sa0l 23.09 067 -. 0.807 e 8.7940.7 7S 7.
?50 0 0 to__ 50 1_0_9 2L 7.183 e0921 0.e13 0.738 V 30 30 14*715 21.73 1 9.033 0.902 0.902 484 X 10 9 14.374 2.43 71.56 0. 71S 0853 0.786 9.
K 30 t0 14.771 23.22 8.449 0.64 0.8453s 413 1
_ __ _ _ _ L 30 30 14.585 22.50 1.935 0.792 _ _ _ _ _ _ _ _ _ _ _ _ _ 0.792 114 t0 to 115.016 22.49 1.404 0.140 03740
-- N 30 30 3481 23.93 9.039 0 906 0.843 8.6 0.906 900 6.757 833S t1A 0 30 ___ 14.314 21.63 6.796 0.850 0.680 P 30 I__ 3 091 Is 22.321.2 0.178 _ _ _ _ _ _ _ _ _ _ _ _ _ 0.702 30t 6 14.586 205 33.664 0 944 05S66 753 ft 30 4 14.398 17.97 3.572 0.893 0.953 4.9 0 357 48.0 8.479 28.9 49A9 S 30 4 34620 38.53 3 930 0 978 0.393
__ T 30 __ 4 14.983 38.98 3.997 0.999 _ _ _ _ _ _ _ _ _ _ _ _ _ 0.400 _ _ _ _ _ _ _ _ _ _ _ _
T-0 14 49 35.56 3084 1.084 03108 V 10 I 35.033 36.0 0961 0.91:704 7.5 00972. 0.131 47.4 84.9 W 30 I 14.578 is557 .992 0RE2 09
_ _ _ _ _ X 30 I ___2 14.712 3698 - 2.266 3.334 _ _ _ _ _ _ _ _ _ _ _ _ 0.227 Deutt's M7D el.1. 0.8301 0.851- W~minnmn signiKe" Dlt¶m"w rMSD: 13.0 ?h8SD- Paerit MinknurnSignniant Differen PMSD Is ameintweofltet preritiom TheJPIMSD i the minim. peuret dift ld 9tl8e,6 3that betwee eotsntro cm be dedwed rtadhicully Otgnidau In awhole effluemtodlely WtL On evange, a dsilece oers {or Prirvironwmnsl 8ifnait~g Tctg Soludlthe..Ihc connic toxckity wthens to..ek reduce,Piwphefe powIb y 36.8%Sm the cots,,3 (delteuned though relremft toxicant teutinI LOwerS4SDbOuMd d&u'wd byUSt A(tO3th petowb) -9.4A tpper 3MSDbonnd detaiained b USEPA(90thpalemhi) - 35%
The lower andupperbomd wereakultd bye USEtA using 20$ tern co08u3ed 6ian19 aboIinries EbrPitnephles growh tI de fCrewce tt r4t te.
USEPA 2000. U af in nder d dAsu g 5eMeodg M W 3 in Whole br Vrlekity tJSTodkeyApp3 mtyed e g;WA roll Dtbtr Elimpoan s A tndo Ay,.5, i.OR O'g- 0 edetthaft sa Aq m-k Df.S)'umi . bse 0"3-0 0 43 0
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 213/2004 Test ID: PPKCICR Sample ID: REF-Ref Toxicant End Date: V1 02004 LAb ED. ETS-Environ. Testing Sol. Sample Type: KCL-Potassium cdloride Sample Date: Protocol: EPA-821-R02-013 Test Species: PP-Pimephales promelas Cormnents:
Cone.mg/L 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000 1.0000 450 1.0000 0.9000 1.0000 1.0000 600 1.0000 1.0000 0.8000 . 0.8000 750 0.6000 0.4000 0.4000 0.4000 900 0.1000 0.1000 0.1000 0.2000 Transrorm: Aresin Square Root Rank 1-Talled Number Total Cone-mg/L Mean N-Mfean lM!ean Min Max CV% N Sum Criticol Resp Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 450 0.9750 0.9750 1.3713 1.2490 1.4120 5.942 4 16.00 10.00 1 40 600 0.9000 0.9000 1.2596 1.1071 1.4120 13.974 4 14.00 10.00 4 40
$750 0.4500 0.4500 0.7351 0.6847 0.8861 13.697 4 10.00 10.00 22 40
- 900 0.1250 0.1250 0.3572 0.3218 0.4636 19.861 4 10.00 10.00 35 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro&Wl-k's Test indicates normal distribution (p > 0.01) *O.91562915 0.884 0.20833591 0.21327986 Equality of variance cannot be confirmed Hypothesis Test (1-tall, 0.05) NORC LOEC Ch1V TU Stee's Many-One Rank Test 600 750 670.820393 Treatments vs D-Control OrganismsobtatnedfromAquatic BloSystems, Inc. 01-20-04
Environmental Testing Solutions, Inc.
Statistical Analyses Maznuim [kl od-Probit
?zrmmfer VAie St 95% Fidual lndit C0 3r0l C20Sq C782ied P.vWab Mu Sltn Ittr Slope 11.861861 1.6t483649 8.55958142 15.1641406 0 3.37020517 7.81472778 0.34 2.86495685 0.0843038 4 ltcept -28.98372 4.82457641 -38.43989 .19.52755 TSCR.
Pn Prebtt n 95Y Fdumiallindts ECOI 2.674 466.484129 389.144941 518.820184 ECMS 3.355 532.462994 465.818241 577392508 ECIO 3.718 571.369662 512.060168 612.024097 EC15 3.964 599.2U479 545.340404 637.1224W EC20 4.15S 622.3074l9 572.874119 658.321118 EC25 4.326 642.828016 597.131193 677.601535 EC4O 4.747 697.587555 659.604031 732.376546 ECSO 5.000 732.751724 696.871674 77L205248 EC6O 5.253 769.688464 733.120462 815.553611 EC75 5.674 833.254644 791.48t734 901.F964S2 ECSO 5.842 t62.797189 t14.442003 940.347615 ECIS 6.036 896.049768 U340175 98t.060218 EC90 6.282 939.715753 875.661298 1052.4946 EC95 6.645 1008.38008 92S.006347 1157.19612 10 100 1000 10000 EC99 7.326 1151.00405 1032.59031 13S5.44355 Dose rngf.
Dose-Resporse Plot 9
la Organbfu obtain edfrom Aquatic BioSyslem, Inc.
01.20-04
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 2/3/2004 Test ID: PPKCICR Sample ID: REF-RefToxicant Und Date: 2/1012004 Lab ID: ETS-Environ. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: EPA-821-ROW2013 Test Species: PP-Pimephates promelas Comments:
Conc-mg/L 1 2 3 4 D-Control 0.8567 0.8203 0.9036 0.8058 300 0.8544 0.7974 0.8067 0.7181 450 0.9015 0.7856 0.8449 0.7915 600 0.7404 0.9059 0.6796 0.7022 750 0.5664 0.3572 0.3910 0.3997 900 0.1084 0.0967 0.0992 0.2268 Transform- Untransrormed I-Tailed Isotonlc Cone-mg/L Mean N-Mean Mean Min Max CV% N t-Stst CriUcal MSD) Mean N-Mean D-Control 0.8466 1.0000 0.8466 0.8058 0.9036 5.152 4 0.8466 1.0000 300 0.7942 0.9380 0.7942 0.7181 0.8544 7.116 4 1.091 2.290 0.1101 0.8125 0.9597 450 0.8309 0.9814 0.8309 0.7856 0.9015 6.513 4 0.327 2.290 0.1101 0.8125 0.9597 600 0.7570 0.8942 0.7570 0.6796 0.9059 13.523 4 1.862 2.290 0.1101 0.7570 0.8942 750 0.4286 0.5062 0.4286 0.3S72 0.5664 21.862 4 0.4286 0.5062 900 0.1328 0.1568 0.1328 0.0967 0.2268 47.362 4 0.1328 0.1568 Auxiliary Tests Stalistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92996466 0.844 0.93001388 0.86128606 Bartlett's Test indicates equal variances (p - 0.50) 2.3672061 11.3448668 Ilypothesti Test (1-tall, 0.05) NOEC LOeC ChV T17 MSDu MSDp NISHI hSB IF-Prob df Dunnett'sTest 600 >600 0.1101369 0.1300932 0.00640092 0.0046262 0.2951633 3, 12 Treatments vs D-Cortrol OrgantsmsobtainedfromAquatic BioSystems, Inc 02-03-04
Environmental Testing Solutions, inc.
Statistical Analyses Unctrthderpthilon (200 1lesam.~)
_.OL SD 95% C__p) S__.w ICOS 472.28 141.80 0.00 690.63 -0.4393
'vtO 586.71 63.26 124.28 650.82 .2.2563
'C'5 617.09 26.83 511.60 664.04 -L1768 1C20 636.42 16.75 583.92 634.53 -0.1181
'C25 655.75 15.41 611.28 705.25 0.1191 X:40 713.75 17.32 671.43 780.64 0.7958 ICs0 752.61 17.31 708.72 810.13 0.5158
.i 0 200 400 600 800 1000 Dos. mg5/L Done-Respe, Plot 0.9 0.8 0.7
~~~~~~~~~~~~~............. 1-tal.
_0.05 1evel m
of significance
<:1 0.5 o 0.4 P.-
0.3 0.2 0.1 0
St 2- § Organ u obralnedfrvm Aquatdc RioSystems. Inac.
02-3-04
Environmental I~vloneta TestinL, Solutions. In.P~
Ts_ n {Soltos Inc. Page 4 of 5 Species: Pimephalespromelas PpKCICR Test Number: 2 4 Daily Chemistry:
. ~Day2 Analyst pitBt 8M =A Concentratio Parameter CONTROL pH (S.U.) ___9 _ 71 __Z3 6 DO (me/L) 5 l8,(. 8 2 7 Conductivity 2 qe (Lmbos/cm) Iq Alkalinity Hardnes<
(mg CaCOV/L) 84___
Temperature 0
____( C) ~ '~ Zqq z'Ie6 ZS.I 7.
pH(S.U.) . 7.8Z 8-03 ThS'4 -.03 -7.6 DO (mg/L) 77._ _ _ ). I 300 mg KC1JL Conductivity (pIMhos/cm) A __ _ _ __ _ _ _
Temperature ZS. % 2.q 24.8 Z5. ZS.3
_ _ _ _ __ _ _ _ ( 0C ) _ _ _ _ _ _
pH(S.U.) S5.Oq T.h)1 &.04 8.oz 8.ez -7.80 DO (mg/L) -7. *; S I ?.
450 mg KCl/L Conductivitymj l 1050 o (gunhos/cm) 15 O~ ___I~r Temperature Z5.1 Z' z
_ _ _ _ (C) ___4A _ Z5____
pH(S.U.) A (lj -Lbq 8.o3 7.3 50( oI -7.
DO(mg/L) 0.Z 7W . a - .
600 mg KCi/L Conductivity 13Y40 (limhos/cm) _ _ _ _ __ _ _ _
Temperature 2i. Z*.jq z .5 Zs. 1 2.$3
_ __ _ _ _ _ _ C C) _ _ _ _ _ __ _ _ _
pH (S.U.)- -Il.q I a-a-Os --713-a X oz01 0 .q5 DO (mg/L) iS).z 8.70:
6-3 i.Z 7 750 mg KCi/L Conductivity lto (OO 16W Temperature 2. 1 z-4. zq.9 Zq.8 ZS. I ZS.3
_ __ _ __ _ _ _ (CC ) _ _ _ _ _ _ _ _ __ _ _ _
pH (S.U.) 0 .qo . .'I 8____
B.O DO (mg/L) 900 mg KCVL Conductivity I l e0o _
(pzmhos/cm) _ _ _ _ _ _ _ _ _ _ _ _ _ _
Temperature 2 .
STOCK Conductivity Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final___
Environmental Testine Solutions. Inc. Page 5 of 5 Environmental Testing Solutions. Inc. PageS otS Species: Pimephalespromelas PpKCICR Test Number: 2 L' Dy 7 5 6 a-Analyst "~4h CmL:Dr Kt516 1 hw 1 K Concentration Parameter . _
CONTROL pH(S.U.) 8.,Z l 841 1F13 t8.ZI 7.6 .It) ___
DO (maL) 7.4 7. (o 63 .I-z6 ___ 1h6 Conductivity (gmhoslcm) Sol 2 5-4 Alkalinity l P U1 (mg CaCO 3IL)
Hardness LA 8 Temperature Z5.1 ;2 4- Z.O ?$ -. 3 z'- Z5.5 25.3
_ __ _ __ _ _ _ (CC ) _ _ _
PR (S.U.) {o -- ?1 1,-7 '4 eG Q.t o -4.1t 6.l e :1.Xw3 DO(mg/L) ,O 1. Lo 8.o -4s 76 :.d evz -
300 mig KCiIL Conductivity M(imhosfcm) 607 8\f4 _ _ _____
Temperature pH (S.U.) 8.__ __98 _ `_ _ (3 O -4 e,-.1 *_ _
DO (mg/L) 8__7_ 74e ___ .eV0.o s3.o 450 mg KCIIL Conductivity 1110 (umhos/cm) II'f0 IO'AO lb1 Io Temperature S. I .p Z5, Z5. C Zl.3 24.6 ZS.T 25,3
)
RH_(S.UC BS. oqp -7 z.sqo 4 0 .o %.-2 s 40e, 600 mg KCUL DO (mgL) _ o 7-7 .8 81j 1.3 6 Conductivity 13ZO
&+/-rnhostc m 13 &o _ _ _ I zo_ _ _ _ _ _ _ _ _ _
Temperature I z. Zs.o Z6.0 2A3 Z4,6 7S.S19 .
_ _ __ _ _ _ _ _ (OC )_ _ _ __ _ _
pH (S.U.) t3.10 176 1.S379°q -,q2 z e.o q --7-7 n9lt ~o DO(mg/L) -7d 8 j -.46 .2 2. 4 750 mg KCUL Conductivity q /
Temperature I Z Z. z,S O zAq,3 2S, 2S e (IC)I p'H (S.U.) a II . L3 '7 Iq1 -4!R a3n1 -I.w_4 9 .1 DO(mg/L) 13,-- 6.? QGI D 900 mg KCIIL Conductivity I6(ao (gmhios/cm) ______ ~ 1 __
Temperature 1 z . Ce 25.0 ZS.O .3 zq. 0:5 3 STOCK Conductivity <
________ ___ (gmhos/cm)
Initial
_nta Final Fa Inltial Sinal
,nta I Initial Final Fi, a_ IInitial
_nta Final ia
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.20 . .. . . . . . . . . . . . . . .
1.15 State and USEPA Mandated ControlLimits Limits Set According to +/-2 StandardDeviations 1.10 I II IIII I II........................ I:
1.05 1.00 ...........- *V...
U cu 0.95 .. . . . . . . . . . . . . . . . . .. . . .. .. .. .. .. .. .. .. .. .. .. .. .. ..
1.5 V
1.4 LaboratoryWarning and ControlLimits 1.3 Limits SetAccording to l dh and 25th Percentile CVs 1.2 ................................................................................................ ..............................................................
1.1 1.0 ,,,,,,,.
0.9 0.8 3.0T 600 S 0\ " s3N O dateOP" 3 'S39 " 00 .1.0 0 ON Test date
-.- 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
- - Central Tendency (mean IC2,)
Warning Limits (mean IC 25 b S^j 0 )
. Control Limits (mean IC,, h SAIS or 2 Standard Deviations)
Environmental Tcsting Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubla using Moderately Hard Synthetic Water State MUSEPA Laberatory Laborstory USEPA USEPA Test nnnrber Test date 7.dsy ICU CT S CeonsslLlnits SuI warning Lbits SAIS ControlLUnits SS75 Wrnn Its SA, CsdrolLlnts CV
(&/LNaCI) (g/LNaCI) CT-2S CT+2S CT-SA, CT+SA., Cr-S,$ CT+S,3A CTr-SAi, CT +rsA Cr- sA, Cr+S, 1 12-03.02 1.02 2 12-03-02 1.03 L02 0.01 1.00 1.04 0.08 0.94 1.11 0.17 0.85 L20 0.46 0.56 1.48 0.63 0.39 1.66 0.01 3 12.04-02 1.02 1.02 0.01 1.01 . 1.04 0.08 0.94 1.10 0.17 0.85 1.20 0.46 0.56 1.48 0.63 0.39 1.65 0.01 4 12406-02 1.03 1.02 0.01 1.01 1.04 0.08 0.94 1.11 0.17 0.85 1.20 0.46 0.56 1.48 0.63 0.39 L66 0.01 5 12-11-02 1.04 1.03 0.01 1.01 1.04 0.08 0.94 1.11 0.17 0.85 1.20 0.46 0.56 149 0.64 0.39 1.66 0.01 6 12.18-02 1.04 1.03 0.01 1.01 1.05 0.0 0.95 1.11 0.17 0.85 1.20 0.46 0.57 1.49 0.64 0.39 1.67 0.01 7 01.07.03 0.96 1.02 0.03 0.97 1.07 0.08 0.94 1.10 0.17 O.AS 1.19 0.46 0.56 1.48 0.63 0.39 1.65 0.03 a 024-03 0.99 1.02 0.03 0.96 1.07 0.08 0.93 1.10 0.17 0.84 L19 0.46 0.56 1.47 0.63 0.39 L65 0.03 9 03-05-03 L05 L02 0.03 0.96 1.08 0.08 0.94 1.10 0.17 0.S5 1.19 OA6 0.56 .48 0.63 0.39 1.65 0.03 10 04-08-03 L03 1.02 0.03 0.97 1.07 0.01 0.94 .10 0.17 0.85 1.19 0.46 0.56 1.48 0.63 0.39 1.65 0.03 11 05-0603 L05 1.02 0.03 0.97 1.08 0.08 0.94 L10 0.17 0.85 L20 0.46 0.56 1.48 0.63 0.39 1.66 0.03 12 06043 1.07 1.03 0.03 0.97 L08 0.08 0.94 1.11 0.17 0.85 1.20 0.46 0.56 1.49 0.64 0.39 1.66 0.03 13 07.0t-03 1.03 1.03 0.03 0.97 1.08 0.08 0.94 .11 0.17 0.85 L20 0.46 0.56 1.49 0.64 0.39 1.66 0.03 14 OS0-03 L04 1.03 0.03 0.97 1.08 0.08 0.95 1.11 0.17 0.85 1.20 0.46 0.57 1.49 0.64 0.39 1.66 0.03 I5 09.10403 L05 1.03 0.03 0.98 1.08 0.08 0.95 1.11 0.11 0.85 1.20 0.46 0.57 1.49 0.64 0.39 1.67 0.03 16 10-07403 1.02 1.03 0.03 0.98 LOS 0.08 0.95 1.11 0.17 0.85 1.20 0.46 0.57 IA9 0.64 0.39 L67 0.02 17 11.04-03 LOS L03 0.03 0.98 1.09 0.08 0.95 1.11 0.18 0.86 1.21 0.46 0.37 1.0 0.64 0.39 L67 0.03 18 12-0403 1.08 1.03 0.03 0.98 1.09 0.08 0.95 1.12 0.18 0.86 1.21 0.47 0.57 1.50 0.64 0.39 1.67 0.03 19 0140644 1.06 1.04 0.03 0.98 1.09 0.08 0.95 1.12 0.18 0.86 1.21 0.47 0.57 1.50 O."4 0.39 1.68 0.03 20 024344 1.06 1.04 0.03 0.9S
CT - Cental tendency (mea IC).
S " Standard deiation ofdto Ic2, Values.
Labortory Central and Warring Units Laboratosy con ad wuninglimitswere estblisheduing the stanard deviationofthe IC,, vlueeseontondbg to the 1Oth nd25thpercentile CV These ange Jre n stdrigt tlthane contml andwming linitsrcomenmded by USEPA for the test method and endpoint.
SA, - Standatd devSationceondlng to the 10"*percntble CV. (SAI, 0.08)
Si,,- Standarddeviationcoespondgto tho25t'peentleCV. (Ss, -0.17)
USEPA Centtral ad Wang Lisrits S, ts - Standard deviation cotrponding to the 75 perveable CV. (SAYS - 0.45)
SAS- Standard deviation coseronding to tae 90' percentle CV. (SAt. - 0.62)
CV - Coetffiiest ofvaiaion ofteIC, Values.
USEPA. 2000. Uudetanding Acotng frMetbodViablity in Whole Efiet Toxicity Applieationa Under the Natoal PollutantDishsao ElimnationPrognam. EPA-33-R-004003. UShivmnmenrtl Protecton Agency, CincinnaiOOIL
)435W
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.8 1.61-1.4 l-%
V 1.2 ........................................................................
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0.4 0.2 N-'4" "O" O' O" v%, so no N-0 SAI 'O" 01 v%5 VqO 0 !Y0
").N t k 1"%, JNVO NO 01- .5%&,
NIP %11% NIA 111 N-0 NI-S %%J1 %I,, 0A to t51A to 14-0 Z*Aq'SN N%q 's0q % x, Test date 7-day IC25 = 25% inhibition conccntration. An estimation of the concentration of sodium chloridc that would cause a 25% reduction in Ceriodaphniarcproduction for the test population.
- - Central Tcndency (mean IC25)
Laboratory Warning Limits (mean IC25 4 SA.IO SA.10 = 0.08)
-- Laboratory ..- Control Limits (mean IC2, I SA2S, SA.5= 0.17)
_ - USEPA Warning Limits (mean IC25 h SA,.75 , SA- 75 = 0.45)
USEPA Control Limits (mean 0C25 h SA-90, SASO = 0.62)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Cerlodaphniadubia using Moderately Hard Synthetic Water Tet Testdat Control Control Mcnn CT CV CT MSD PMSD CT nurnber Sunrival Reproduction for Cc ror Control
(.) (offsprinog/emale) Rep ('f) Reproduction (%e) forPMSD (/.)
(offep2 CV (.)
1 12-03-02 90 34.0 8.0 2.7 8.0 2 12-03-02 100 33.2 6.2 7.1 3.3 9.9 9.0 3 12-04-02 100 32.5 6.0 6.7 3.2 9.8 9.2 4 12-06-02 100 29.7 11.0 7.8 3.0 10.0 9.4 5 12-11.02 100 33.8 13.7 9.0 2.9 8.5 9.2 6 12-18-02 100 30.5 7.4 8.7 2.9 9.4 9.3 7 01-07.03 100 33.2 7.0 8.5 2.9 8.6 9.2 8 02-04403 100 32.3 8.1 8.4 2.7 8.4 9.1 9 03-05-03 100 28.7 5.1 8.0 3.5 12.1 9.4 10 04-11-03 100 26.3 6.2 7.9 2.5 9.6 9.4 11 05-06-03 100 27.6 10.8 8.1 3.2 11.5 9.6 12 06-04-03 100 25.9 5.9 7.9 2.6 10.1 9.7 13 07-08-03 100 29.0 11.6 8.2 3.2 10.9 9.8 14 0i-05.03 100 33.3 6.6 8.1 4.7 14.1 10.1 15 09-10-03 100 29.3 4.3 7.9 3.1 10.7 10.1 16 10-07-03 100 33.4 8.0 7.9 3.2 9.5 10.1 17 11-04-03 100 31.0 7.3 7.8 2.6 8.3 10.0 18 12-04-03 100 30.4 9.7 7.9 3.6 11.8 10.1 19 01406-04 100 30.6 4.4 7.7 3.5 11.5 10.1 20 02-03.04 100 30.7 7.2 7.7 2.7 8.8 10.1 Nole: CV - Coefficient of vaziation for control reproduction.
On average, the CV for control reproduction is 7.7% in Environmentsa Testing Solutions. Inc, Certodcphnta chronic toxicity test.
Lower CV bound deterninedbyUSEPA(10'permctile) - S.9%.
Upper CV bound determined by USEPA ( 9 0a perentilc) - 42%
MSD - Mnimnum SignificantDifference PMSD - PercentMiniSu Significant Diference PMSD isa measure of test precision. The PMSD is the mirimum percent difference between the control and treatment that can be declared statistically iignificant in a whole effluent toxicity tesL On average, a significant difference occure for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphniareproduction by 10.1 %
from dte control.
Lower PMSD bound detcrrinedbyUSEPA (10' permcetile) -11%.
UpperPMSD bound determined byUSEPA(90pecentble) - 37%.
CT - Central Tendancy (Mean Control Reproduction. CV, or PMSD)
The lowerand upperbounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphniareproduction in chronic reference toxicant tests.
USEPA 2000. Undertnding andAccounGng forMethod VariabilityinWboleEffluenlToxicityApplicatons Under the National PollutantDischae Elimination Program. EPA-833.R.00-003. US Environmental Protection Agency. Cincinnati, OH.
02-03-04
Environmental Testing Solutions, Inc.
Ceriodaphniadubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests 40 1:--I .
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_..p si...g .. .fe _ __e Minimum Acceptance Criteria (IS5.O offspring per surviving female)
I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 40 North._.._.._a.A.cep.ance.Lim.t.........
.-- NC North Carolina Acceptance Limit (< 40.0%)
C, 0 30 o -6 Kentucky Acceptance Limit (< 30.0%)
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- Control Reproduction or Coefficient of Variation (CV)
- - Central Tendency (mean Control Reproduction or CV)
... Control Limits (mean Control Reproduction or CV 1 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphntiadubia using Moderately Hard Synthetic Water so 45 40 35 30 25 20 15 _
5 0 I I I I I I I I I I I Test date
-. PMSD percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant
- - Central Tendency (mean PMSD)
.........Lower and Upper PMSD Bounds Lower PMSD Bound (le" percentile) = 11%, Upper PMSD Bound (90"' percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Environmental Testing Solutions, Inc. Page I of 6 Environmental Testing Solutions, Inc. Page 1 of6 Jim Sumner I attest to the accuracy and Integrity of this document 2004.02.14 17:08:39 -05'00' Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia CdNaCLCR #: Ij DilutionpreparationInformation: Comments:
NaCI CHM number. (e 1.4lr 01 Stock prparation: 100 g NaCIA (dissolve 50g INaCI in 500 ml deionized wate)
Dilution p60p (m0lL) . _S00 1000 l 1200 . 1400 Stock volume (mL) 9 12 115 1 IS I 21 Diluet volumeNO 1491 1485 1485 1482 1479 Total vol5 (mL) 1500 00 1500 1500 1500 Test organism information: Test information:
Organism age: . <24-hours old Randomizing template: &..e.
Date and times organisms OZ-O-o!-4CA o51 *M 10Q' Incubator number and 2. r were born between: shelf location:
Organism source: 1-ZI-0C A-1b YCT batch: ABS I-22-2-o' Transfer bowl information: pH -. IISU Temperature =?LCGC Selenastrum batch: ABS o1-22-14 Daily renewalinformation:
Day Date Test initiation, Control watcr batch Analyst renewal, or used termination time 0 Oz-02 .oq .3. p2 02 z -z.oq
_ C> 02z-az-,
O 3 D 0 1100 oz__s_-o__
4 1).T-bl-CA 11__
_-____ I__
5 oZ-t*8o' I 4O 027t04O t 6 - - -0, 4 IOC).40 oz-OZ(6-Controlinformation: Acceptncec critera Summary of test endpoints:
MalecAdults:
__f _o _ 520%Yo S_ 7-day LC50 l IO 0O
%Adults having 3r Broods: . B0% NOEC /
% Mortality: s 20% LOEC 1 Mean Offspring/FCVnale: 1 .7 2 ofrpnZ/ferale 4 0.0 ChV 1
% CV: -7. 2%o 40.0x. IC25 /,-
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphniadubia CdNscCLCR: jcj CONTROL Survival and Reproduction Data Replicate number Day _ t 1 _2 3 4 5 6 7 8 9 10 Young produced Q O _) o 0 QQ) 0 L-.J Adulttmortalty L L- L L L-2 Young produced O CQ. 0 0C ._ ) (
Adult mnortaity _ L1 l_ L- j 3 Young produced j 0 LA I 6s L44 x Adult mortality1 l1_ l 4 Young produced (o O4
( c. 1 _ o &LJo .'
Adult mortality L.- L . . L-IL- ' L L- L-5 Youngproduced 10 _I I 12_ 10 IlX I cJ i 7 43 Adult morta11ty _ L_- L- ll t 6 Youngproduced l 0 O
) C C C l o l Adult mortality I L.- L 1 L- 1ll 77 Young produced I's IS t 1 t15_ Sl1 IS I-s l:Z l- I LA Total young produced l 3 30 l 30 l 2Cn l z l l31 Flnal Adult Mortality L( T7 L- L-T T L- i L i LL-Xfor 3" Broods T X >- I )< l >C > I >c
.7 - -- -
I Concentration: I
%Mortality: , Oo l Mean Offspring/Fenale: .3 10 600 mg NaCL/L Survival andReproduction Data RePlcate number Day l 1 2 3 4 5 6 8 9 ID 1 Youngproduced 0 0 0 IT Adult mortality lL L L L L- L 2 Young produced l) OlC - - ) ' S Adult mortality l L l l ll L Young Y
Adult producedl mnortality l l l l l-l tl - r CI sos L 0s '5 4 Young produced . lL)S 0 0 - l l 4* C5 1 Ve.-
s Younngproduced lt °cl IA I 1l 1I l I ..
Adult mortality U - L-_ L- tU- L- 1 - lL Ll- L Adult mortality . L U. U .. L . U. T 6 Young produced L- ~ C -- I1 Totalyoungproduced 29 l3i l l l 3c 22 2t9l Final Adult Mortality L i T L i U - I L L l Concentration: 076
%Mortalit:y I Mean Offspring/Female: I3o.2 I
%Reduction from Control: Il. toL I
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphniadubia CdNaCLCR #: Iq 800 me NaCI/L Survival and ReproductionData Replicste number Day I1 2 3 4 5 6 7 l 9 10 I Young produced 0 l 1 I a l
- Adult mortality L_ Jr L _- _ ( . -L 2 Young produced _ l J)0 J.)12 O L AŽ....j..@ C Adult ort__sy
- _ L I _ _ I _
3 Young produced 4 r O I S OQ . Q Adult mortalIty L- I '.
4 Young produced j) l I S S C)
Adult mortality L . . U_ U L U l S Young produced L I0 10 1) 13 10 *C) I .i Adult mortality t 1 L . l. L U-6 Young produced . 0 C) I t . ._ Q Adult mortality t . . L-_ j U 7 __Youngtproduced
_ __ lIkeI IL I9 19 _3 1 5q3 \-\1 4- 1-Totalyou ntproduced l- Zl 5\ 3 OO
-6 l ze 30 2&
Final Adult Mortalit_
Concentration:
% Mortality: . C .1 Mean Offspring/Female: I 95
% Reduction from Control: 3'1%
1000 mg NaCI/L Survival and ReproductionData RepUcste number D _ l 1 2 3 4 5 6 l 7 8 9 10 I Young produced l () l l 0
. Adult mortality LI- L La I - a 1L n a L- a 1 2 Young produced )) C 0 Adult mortaUty (.. T _ I L L L.,- F 3 Yowng produced Adult mortality LA L.U 5 J-t)
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. Z 4 Young produced () -=+. - i C"v Adult mortality l - l L. L- I l- LI U I El . I S Young produced lo it* to1 Il 1;2.. Ic) .1...!L.
Adult mortality l - l U - L-L- L- L i- k L- l U 6 Youngpropuced 0D 0
°°h . 0 t* Ci iOU Adult mortality L -
- L t L- L- I j, I 7 Young produced 1I 15~ 12o
( . I.3 I12.. __ 11 2 Totatlyouug produced 32. l I a 2 (P 2C l 28 l Z& Zl l Finul Adult Mortality I
- I LC t- I L I L l It L I Concentration:
I %Mortality 0V I lMeandOffspring/Femate: l A o l% Reduction from Control: l5.9 'lol
Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphniadubia CdNaCLCR#: __19 1200 ma NaCIJL Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 l 8 9 10 1 Young produced Ql () l f) I O 101 O ( l l_oU Adult mortallty L U.- L L L L 2 Young produced Adult mortality L* L Ul 3 Adult mortality Young produced L _ Lc> O ii 0 ) CS O LCa)3 C, 4 Young produced . I O0 Q 3 S Adult mortality Young produced *1 il il ol 1 f o o l 1 Adult mortality . U U l - L- 1 6 Young produced *1 T lo -. I o 0 SI Adult mortality LL- L- L- L-'-
7 Young produced 10 10 (o 0 Is Liq .
Totalyoungproduced T 1i l CO 12.z T1 FinalAdultMortlity l . t_ l ? U.
- Concentration:
l% Mortality, 07.o I Mean Offspring/Female: IIC5
% Reduction from Control: (d.I 1400 mg NaCllL Survival and ReproductionData Replicate number I l Day l 1 2 l 3 l 4 l 5 6 l 7 l 8 l 9 l 10 l I Young produced Q o l1 0 Q 0
- Adult mortality LJ1 L LI I 1 U I I L L 2 Young produced l l C)
Z l l J L . -
Adult mortality l T -
3 Young produced ) 0 . . . 0 1.x .. 3O l Adult m ortality U ... J T I f . ~ U 4 Youngproduced l Z. l 3 lO 0 _O O 0 Adult mortality I L I 1 l L U.- I . L U1 l 'Youngproduced 2. C) .je... 1C 1 O 0 Adult mortality - . U . . l l ( -I U 6 Yo n produced Q Q
- ZE i I. .
Adult mortality U U. U . L. .U 7 You ng Produced Total young produced FInal Adult Mo tliyU I 0
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% Mortality:
Mean Offspring/Female
% Reduction from Control:
r -LI a&.'.qi
Environmental Testing Solutions, Inc.
Verification of Ceriodaplhnia Reproduction Totals Control 1000 mg NaC1/L Pay Re lnte number Total i Da l _Re H1ceate number Total yI l2 3 4 5 6 78 9 10 Tot 2 1 3 1 4 5 6 1 7 81 91 10 a o Io o-o - o - - o - I1 0 o o o o 0o o o o I o0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 O 0 o 0 0 0 0 0 0 0 3 0 0 0 4 5 4 0 4 4 4 25 3 4 5 0 5 0 0 0 0 0 4 IS 4 6 6 4 0 0 1 4 0 0 0 21 4- 0 1 5 0 4 4 5 4 4 0 27 5 10 13 11 12 10 1 10 13 10 13 113 5 10 11 11 10 10 121 10 9 10 10 103 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 1 0 0 0 1 7 17 15 16 14 15 15 12 15 15 14 148 7 16 15 15 16 12 13 12 15 12 14 140 Total 33 34 31 30 30 31 26 32 29 31 307 Total 30 32 31 31 26 28 2 26 23 2S9 829 600 mg NaCI/L 1200 mg NaCI/L Day Re plicite number = oa Rep hcate numbtr lDa Total
- 2 3 4 5 6 7T 8 To9ta D1 - 2 3 1 4 5 6 1 7 1 8 9 10 1 0 0 o 0 0 o o00 o 0 I 1 0 0 0 o0o 0oIo 0 0 0 0 2 0 0 0 0 0 0 0 0 0 _0 0 0 0 0 0 0 0 0 0 0 0 3 4 4 0 5 4 0 4 0 5 4 30 3 0 0 0 0 0 0 0 0 3 0 3 4 0 0 S 0 0 5 0 5 0 1 16 4 0 2 0 0 0 0 0 3 0 0 5 5 10 14 10 11 -10 11 10 13 11 12 112 5 8 5 7 6 0 6 2 S 0 5 44 6 0 0 0 0 0 0 0 000 0 6 0 0 0 0 0 O 0 0 0 0 0 7 15 13 14 15 14 14 16 14 17 12 144 -7 1010 6 0 5 4 6 4 2 6 53 Total 29 l31 29 31 28 30 30 32 33 29 302 Total 18 17 1 13 6 5 10l 8 12 5 11 105 800 mg NaCI/L 1400 mg NaCi/L Re licate tiumber Day Day le PIlcate 1umber , , Total 1 2 3 4 5l 61 7 8 9 10 Total I1 2 1 3 1 4 1 6 1 7 8 I 9 0tO
-- I 0
°- 0 -° 0 0 0I 0 0°- -0 0 0 0 -- I
-° °- °a 0 0 o 0 °0 0 0 o 0a I o 0 2 0 0 0 0 0 0 0 0 0 0 0 2 O 0 0 0 0 0 0 0 0 0 0 0 3 4 0 4 4 5 0 0 0 0 0 17 3 0 0 0 0 0 0 0 3 0 0 3 4 6 0 0 0 5 4 5 5 4 29 4 0 2 3 2 0 0 0 0 0 0 7 5 11 10 10 12 10 10 13 10 11 10 107 5 2 1 6 5 0 2 0 0 2 0 18 6 0 0 0 0 0 0 0 0 00 0 6 0 0 0 0 0 0 0 1 0 2 3 7 16 14 14 15_ 15 13 14 13 14 14 142 7 0 0 0 0 2 0 0 0 1 0 3 Total 31 30 28 31 30 28 31 28 30 28 295 Totll 2 31 9 17 2r 34
Environmental Testing Solutions, Inc.
Chronic Whole EMuent Toricity Test (EPA-821-R-02-013, Method 1002.0)
Species: Cerlodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNrCICR US (#19 at 3S1 Depot St.)
Test dates: February3. t0, 2004 Ilevetved b Concentration - Repikte number _ Survival Average reproduction C~mde..I f rP.~lrodWO ftm (mg/LMCI) _ 2 3 4 5 6 7 8 _ S (%) (offsprlngftemuale) "u*e (%) tesube (4)
Control 33 34 31 30 30 31 26 32 29 31 too 30.7 7.2 Not appllehab 600 29 31 29 31 2S 30 30 32 33 29 100 30.2 5.1 1.6 800 31 30 28 31 30 21 31 2S 30 2t 100 29.5 4.6 39 I000 30 32 31 31 26 29 21 2S 26 21 100 2J 9 7.2 5.9 1200 is 17 13 6 5 10 S 12 5 It 100 10.5 44.3 65.8 1400 2 3 9 7 2 2 0 4 3 2 90 3.4 78.7 J8.
Dunnett's MSD value: 2.713 MSD - Minimum Significant Difference PMSD: 1.8 PMSD - Percent Minimum Signifcant Difrerence PMSD Is a measure oftest precision. The PMSD is the minimum percent diferernce between the control and treatment that can be declared statistically significant inwshote efluenttoxlcitytest On avenge aslgnficantdifrercnce occur bfrEnvironnentalTesdng Solutions. Ine chronic toxicitytests when a toxicant reduces Cerlodaphnfa reproduction by 10.1% kom thc control.
Lower PMSD bound detennined by USEPA (10' pentmile) - I 1%.
Upper PMSD bound determined byUSEPA (90' percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratoties for Cvrodaphnisreproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Vsrsbilityin Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA433-R . US Ervironental Protection Agency, Clncinnatl. OH.
Environmental Testing Solutions, Inc.
Statistical Analyses Cerlodaphinla Survival and Reproduction Test-ReprodUctiOn Start Date: 2/3/2004 Test ID: CdNaCICR Sample ID REF-Ref Toxicant End Date: 2/1012004 Lab ID: ETS-Environ. Testing SOl. SamPlO Type: NACL-Sodiurn chloride Sample Date:. Protocol: EPA-821-R02-013 Test Species CD-Ceriodaphlia dubia Comrnents:
Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 33.000 34.000 31.000 30.000 30.000 31.000 26.000 32.000 29.000 31.000 600 29.000 31.000 29.000 31.000 28.000 30.000 30.000 32.000 33.000 29.000 800 31.000 30.000 28.000 31.000 30.000 28.000 31.000 28.000 30.000 28.000 1000 30.000 32.000 31.000 31.000 26.000 29.000 28.000 28.000 26.000 28.000 1200 18.000 17.000 13.000 6.000 5.000 10.000 8.000 12.000 5.000 11.000 1400 2.000 3.000 9.000 7.000 2.000 2.000 0.000 4.000 3.000 2.000 Trnansorn: Untransrormed lank I-Tailed IsotoniC Cone-% Mean N-Mean Mean Min May CV% N Sum Critical Mean N-Mean D-CoXtrOl 30.700 1.0000 30.700 26.000 34.000 7.210 10 30.700 1.0000 600 30.200 0.9837 30.200 2S.000 33.000 5.130 10 93.50 75.00 30.200 0.9837 800 29.500 0.9609 29.500 28.000 31.000 4.590 10 84.50 75.00 29.500 0.9609 1000 28.900 0.9414 28.900 26.000 32.000 7.194 10 82.00 75.00 28.900 0.9414 1200 10.500 0.3420 10.500 5.000 18.000 44.274 10 55.00 75.00 10.500 0.3420
- 1400 3.400 0.1107 3.400 0.000 9.000 78.676 10 55.00 75.00 3.400 0.1107 Auxiliary TexLi Statistic Critical Skew Kurt Kolmogorv D Test indicates nomml distribution (p > 0.01) 0.86754763 1.035 0.426587 1.19887175 Bartlett's Test indicates unequal variances (p - 2.50E403) 18.3882027 15.0862722 Hypothesis Test (1-tail, 0.05) NOEC LOEC CIV TU Steel' Many-One Rank Test 1000 1200 1095.44512 0.1 Treatments vs D4Control 02-03-04
Environmental Testing Solutions, Inc.
Statistical Analyses Linear Ilrpuatdon(OO Restnles)
PoW SD 95% CL Skew XC05 91L666667 159.295919 443.616279 1011.1882 -1.0361 MC10 1013.80435 26.6511843 92,42 1026.83876 -4.9868 1030AS913 7.62826375 1012.93449 1043.2405 *0.1793 1.0 IC20 1041.17391 7.55412408 1030.88297 1059.54745 .0.0737 0.9 1063.2587 7.74712031 1048.91793 1076.13861 0.0293 ICAO 11 3.91304 9.66174513 1097.05444 1132.31828 0.2778 0.8
'C40 1147.28261 11.6603508 1128.27576 1171.16036 0.3831 n 1 0 500 1000 1500 Oose %
Dose-tRtsp e Plot C 25 a 20 10 0.
a15 02-03-04
Environmental Testing Solutions, Inc.
Statistical Analyses Used for PMSD calculation only. Cerfodaphula Survival and Reproduction Test-Reproduction Start Date: 2/3/2004 Test ID: CdNaCICR Sample ID: REF-RefToxicant End Date: 2/10/2004 Lab ID: ETS-Environ. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: EPA-821-R02.013 Test Species: CD-Ceriodaphnia dubia Comments.
Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 33.000 34.000 31.000 30.000 30.000 31.000 26.000 32.000 29.000 31.000 600 29.000 31.000 29.000 31.000 28.000 30.000 30.000 32.000 33.000 29.000 800 31.000 30.000 28.000 31.000 30.000 28.000 31.000 28.000 30.000 28.000 1000 30.000 32.000 31.000 31.000 26.000 29.000 28.000 28.000 26.000 28.000 1200 18.000 17.000 13.000 6.000 5.000 10.000 8.000 12.000 5.000 11.000 1400 2.000 3.000 9.000 7.000 2.000 2.000 0.000 4.000 3.000 2.000 Tarnsforn: Untrzzsrorwed 1-Talled Conc-% Mean N-Mean Mean Mlt Marx CV% N t-Stat Critical MSD D-Control 30.700 1.0000 30.700 26.000 34.000 7.210 10 600 30.200 0.9837 30.200 28.000 33.000 5.130 10 0.421 2.287 2.713 800 29.500 0.9609 29.500 28.000 31.000 4.590 10 1.012 2.287 2.713 1000 28.900 0.9414 28.900 26.000 32.000 7.194 10t 1.517 2.2S7 2.713
- 1200 10.500 0.3420 10.500 5.000 18.000 44.274 tO 17.027 2.287 2.713
- 1400 3.400 0.1107 3.400 0.000 9.000 78.676 10 23.012 2.287 2.713 Auxliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates nonmal distribution (p >0.01) 0.86754763 1.035 0.426587 1.19887175 Bartlett's Test indicates unequal variances (p 2.5013-03) 18.3882027 15.0862722 Iypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDn MSDp MSB MSE F-Prob dt Dunneti's Test 1000 1200 1095.44512 0.1 2.71276873 0.0883638 1449.52 7.03703704 7.1E-34 5, 54 Treatments vs D-Control 02-03-04
Environmental Testing Solutions, Inc. Page S of 6 Species: Ceriodaphniadubia CdNaCLCR#: 11 Daily Chemistry:
Day
, Analyst _ ( c C cm.
Concentration Parameter .________
pH(S.U.) _Oq S.og .07z 8.o B.qo DO (mg/ti 15. ~ 2 q7 CON'TROL Conductivity (pmnhoslcm) 30 JOS Alkalinity (mg~ CaCO3/L) 0 Hardness
( C0C 3 fL) ___
Temperature
(°C PH (S.U.) .OD 7.q 6 'I1- 24.5Z7.18 24.
DO (mg/L)_ _ a8,1 w 6 A 600 mg NaCI/L Conductivity 13eo (Ounhos/crn) ___ ___ __ _ __ _ __ _ __ _ _
Temperature 2 t 61
- 2qVl pH (S.U 0.05 1.q(O 6 O5 -7.' -7CI t(,
7A DO (mg/L) _._ _ 8t _ _ . _8 V __
800 mg NaCVL Conductivity 20240 O40 O (Amhos/cM) ______ _ _ _
Temperature 7 zlbl C0C) zS.O 0 -qzq 24 L,. I6 z.
pR (S.U.) - 7.9-1 .07 -7.qO c -7.*q-=
DO (mg/L) __ ___J 7-el 1000 mg NaCVfL Conductivity i qO Zlq1 (uimhostern)_____
Z, 4e zq-qz .S Z t3 Temperature ZY-A
_ __ _ _ (CC) ZS.O Z~L.t S ZLU8 z4 pH (S.U.) B_.
_3____ __7_ _
DO (mglL) 0.1 -7.4j .C8o .
1200 mg NaCI/L Conductivity
(.unhos/cm)_____
Temperature
_ __ _ _ (CC) n .o q Zq. z f- -Z f.S z4a .e-pH (S.l)8O IA(1O b8.ok Iq I. -.C7 L '7-q(o DO(mgL 9.Z ..74 _m _ R9 1400 mg NaCVL Conductivity zeeo - zq'.' o (pimhoslcrn)
Temperature
_TCKC___ _ (
STOCKConductivity
____ ______ ___ (prnh S/Crn)
Initial- - I Final Initial -
I -
Final - -
Initlal
-- J- Flnal
Znvironmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphniadubla CdN'aCLCR #: 19 I
3 4 5 l6 Ll1 iAnalyst CA(
Concentration 044Y I Parameter ii4 bA l*Y l/tyk4a* V m = 9" 0 .U
.pH (S.U.) H DO (mWQL) 19.2 B.b 83t_
{.11 7 .ZA 6.1 5.2I 8os -o 8-o0
-4.B CONTROL Conductivity 753051; 0
4.15 2154 w
- 3_.1
. H(inS.U)s.1zi Temnperature .S0 0CS,.13 n
~ aC/
600 ~ ~ Co mdctiiy m CO Ol3 8lt(sOs DO (mg/L) t3.C885 OA3e9.0 00 6-0 -4.
6 mg NaCiL 00 Conductivity 13.7 pH (S.U.) Zqq 15.(o 0 B- M 1: 8.15 Oao f -1 i10 ae -Cq -4.s -. R I.son Tem~perature zq9 zll 4a Z.1alv 4 S> Pv
.DO(mg/L) g Z o 83 .i.
l 100mg NaCitL
_pH (S.u.)
(Cporfnductivi~tyZ6 1XTemperature la-O -I.Ct 2O 8B.00 e 2w 6D. B.0 A M31 t 2 e~
loG-0 Q.o 3
A.
ZQ.4 7 1eZ147Zq.eZ.6(z Y.-:1 zq.9 zA4 1 pHx(S.U.) a8.00 312- 7 '+.q 6.z 193>~ 11.o L DO (mglL)
_*Temperature 2 et )-° Bo C ,8.E 7 :3Z -41D4-8 (*l Z.3.Z 6- Z515.l Zq.78.3 STOC(mgondu/Liv)t-1400 mg NaCI/L Conductivity t 7 13ltm_,, a _oI. 67 2qo 2gD 2qzOU0 mgn Initia nitial S/m V04 l0bzBwz ICFinaltFinal 7nittal Yniff-al 11 171ned TnIttal 1PIn2l Inift2l I Final rinal VI . .n.- I I Final-
.... -F I* .- #1VcLVxU0011 IIulliefentJ ht tONALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Address P.0.110OX2000 ____________ (SUBR 01) 0MB No. 2040-0004 I TN0026450 103 G I F - FINAL
_ . . SQDjY-DAISY TN 37384 - PERMIT NUMBER IDISCHARGE NUMBER I LOW VOL. WASTE TREATMENT POND
_d(y_ VA -SEOUOYAH NUCLEAR PLANT----
Locaon _AMILTN CO UNTY _________
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-I I I EFFLUENT ATTN: Stephanie A. Howard YEAR l MO DAY oYEAR m NO DISCHARGE LII NOTE: Read Instructions before cornoletino this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE
_______EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE **.7.0 14129 GRAB MEASUREMENT 8.5 12 00400 1 0 0 . '. PERM!'.t
- s u _9 0 EEA -
EFFLUENT GROSVAU REURMNi M. N *"%MUM SOLIDS,TOTALSUSPENDED SAMPLE 184 301 26 17 24 19 O 6129 GRAB MEASUREMENT 2 00530 1 0 0 LBS5DY !vY ko ' MG/L _ WEEKLY G B EFFLUENT GROSS VALUE -; MOAG DAYMX __________zMOV DAL X' 2 's.
OIL AND GREASE SAMPLE <54 <68 26 <5 <5 19 0 6/29 GRAB MEASUREMENT 00556 1 0 0 M.BS/DY MGI WEEKLY G EFFLUENT GROSS_____________
VALUE -4MRQIREET. M~ ___
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FLOW, IN CONDUIT OR THRU SAMPLE 1.182 1.640 03 *e*h 291/29 TOTALZ TEATMENT PLANT MEASUREMENT EFFLUENT GROSS VALUE -REQUIREME4RTT 'REOT1 GD ",'*DAIL' . ~ ~ ~ *.r MREASUREMENTi SAMPLE SAMPLE MEASUREMENT MEASUREMENT SAMPLE MEASUREMENT MREASUREME'NT' e NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CarliyuIrder penalty of law that docuant rNs and *It attacimentswere preparedunder7m I ' r l TELEPHONE DATE Richard T. Purcell directOn orfsupervision in accordance with a syslem designed to asure that qualified peren Propery gather and evealate the Wodnrmatlon suubmitted. Based on my Inqulry of the person or nl SieVc Peietpersons who manage the System, or thoe persons directly responsible for gathering ft Principal Environmental Engineer 42 83-70 4 03 -
infonyalion the iormation submitted is h to the best of my knowiedge and belief. tmeP 423 843-6700 04 03 09 Sate Vice President ccurate. and complete. Iama&ware that there ae sigfnlicant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I TIDormation. incairg the possibiity of rmneand imprisonment for knoting violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I _______________
I___________________________ CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
TBS was aligned to YP on 2/9/2004 from 0950-1610 and on 2/17/2004 from 1020-1707.
EPRA F-orm JJZU-1 (REV 3l/99 Prevous editions may be used Page 1 of 1
..V iLLV1 ul:.tAvtKbL hLIMINAIION SYSTEM (NPOESI MAJOR Fonrm Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address P.QBBoX 2000 - - - ______
__ _QTE0MFFIE!& S- -2 -- I TN0026450 107 G F - FINAL
___SODDY -DAISY TN 37384 I PERMIT NUMBER I I DISCHARGE NUMBER METAL CLEANING WASTE POND LaJI__ WA.SEi6OAH NUCLEAR ANT______
Locaton _AMILTON COUNTY ________ M1ONITC RING EEIEFFLUENT ATTN: Stephanie A. Howard From o
04 MO I l0 To YEnI 0lMOIDA
- NO DISCHARGE 1 .11 NOTE Read Instincions before completin this form.
I.
I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penatty of law that this document and all attachments were prepared under MY / TELEPHONE DATE Rirection or spendsion h accordance vitlh asystem desIgned to assure that qualified personnel Richard T. Purcell property gather end evaluate the kformation submitted. Based on my ncuiry of the personor_
pesornsvwo manage the system. or tiose persons directly responsible for gatherhig the P"icipal Environmental Engineer 4 inrmtion. the information submItted Is .o th best of my krvwedoe indbelief, true Pnncpaa 423 843-6700 04 03 09 Site Vice President auate. and compiete I em aware that ther are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE hnormation Icludin wthe possibility of ine nd iprrsoonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ._CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ttachments here)
Metal Ponds were released from 2V12/2004 through 2116/2004. No phosphorous bearing cleaning solutions were used.
EP Form EPA For 3320-1 _RE 3199) 30.1_ (REV 3.9 P_ .u _dtin m. be.use Pfevious efflOns may be used Pae o Page I of I
... .. ____ w...'-_u WLuuidtIn it LJrrerenh) e I ivIwmv NAIIONiALPOLLUTANT DISCHARGE ELIMINATION SYSTEM (NPOES1 MAJOR Form Approved.
Name __A - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOY 2.00
____ff E[ROFFIEZ .BB_
.---- .. I TN0026450 110G F - FINAL
___ _SODD TN 37384 _______
_TAISY I PERMIT NUMBER ldy AA-.SEOUOYAH NUCLEAR PLANT_
DISCHARGE N R RECYCLED COOLING WATER Location HAMILTN C U -J_---__---__ I MOtNiITfntING(- painn PRO EFFLUENT YEAR MO I DY o YEAR MT ATTN: Stephanie A. Howard From 04 102 l 01 ITo 04 102 l 29 Y
- '* NO DISCHARGE [Fxfxi ...
NOTE: Read Instructions before con NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty oftaw that iths documnt and alt attadyents were prepared under my . l TELEPHONE DATE direcion or supervislon In accordance with a system designed to assure that qualified perser" e 1 O.
I) C O,6/Av-I41 Richard T. Purcell property gather and evaluate the Information sumrnitted. Based an my Inquiry of the person or UWLutc
_ . dfiv _.
persons wtho mane athe system, o thse persons directly responsible for gathering t PiPnncipal Environmental Engineer iniformation. the Information submitted Is. to the best of my knowledge enadberief. true, Enginee70r0 0 09 Site Vice President accurate, and complete. I am aware that atre e significant pernatlies for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI information, including the possibility of fir ernd imprisortmen for ktowig viatlios. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY ViOLATIONS (Reterence all attachments here)
No Discharge this Period EPA Form 3320-1 (REV 3(99) Previous editions may be used Page 1 of 1
- -j iMAJUK urns lipp"yeu.
DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-0004 Address . 0 . . . . . .
_ __ _QqTEROffiqE S..? . . . . . . . I TN0026450 116 G F - FINAL
____-ODDY iAISY_ TN 37384 ______ I ERMITNUMBER..I DISCHARGE NUMBER BACKWASH Lad! _ TVA-SEUOYAHNUCLEAR PLANT Location HAMTON CO_ UNTY _________ I MONITORING PFRIOD I EFFLUENT ATTN: Stephanie A. Howard YEAR I MDI QAI YEAR, MOT From 04 l 02 l 01 ,i To 04 l02 l 29 I *** NO DISCHARGE E] ..
NOTE: Read Inshictions before con rer penalty odtaw that this doiument aid an *ttadiments were preptred under my .
NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I 1eireodon or supervision In accordance with asystemn designed to essure that qualified personnel Richard T. Purcell *her and evaluate the Iformaieton submittled. Based on my hncquiry h
of the person or manae*th system, or those persons directly responsible for gathering ft Pinclpal~E -4nm cl e Information submitted Is. to the best of m~y know'edge end belief. true. Pnpal Environmental Engineer Site Vice President id complete. I am awere that there we signiticant penalties for submiitting false SIGNATURE OF PRINCIPAL EXECUTIV "uOTWln, Inclirlng the possibility of rne and imprisormnerit for knowing violations. OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reierence arrafachmenten here)
Operations performs visual Inspections for floating debrIs and oil and grease during all backwashes.
ARE 3199) 330. (REV Form 3320-1 EPA Form 3nS\199) editin may PAviu editions my be use be....... Pag __of. 1 EPA Previous used Page 1 of i
- ... v.,L.u1t,0, uIOtUlAHUE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TvA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMM (SUBR 01) OM8 No. 2040-0004 Address P.OBOX 2000
_ . .I E1H:ROFF11E-S8R - : - - - - - - - l TNO026450 1 117 G F- FINAL
___SODDY. DAISY TN 37384 PERMIT NUMBER Failt WA. .SEOUOYAH NUCLEAR _LANT___
I GE NUMBER BACKWASH OLcaon HAMILT ONC QU NT__________ MONITORING Pm EFFLUENT ATTN: Stephanie A. Howard From YEAR MO 04 I-02 1 01 I DtAY To IYEAR LMO 0O4 02 DAY 29
- ' NO DISCHARGE m *.*
NOTE: Read InstruclIons before con NAMEiTLE PRINCIPAL EXECUTIVE OFFICER I Certity under penalty of law that this docent idat siladvtets were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assue tht qualified personnel ,a ,
Richard T. Purcell property gather and evaluate thwtnformatton submitted. Based on my Iuy of the person or personstwho manage the system, or those persons directly responsible for gatherkig the PrincIpal Environmental Engineer information. the information submitted Is.*to fth best of my knro~etdge andl beflef. true, 423 843-6700 04 03 09 Site Vice President acourate and complete. I am eware that there rre significan penaties for submnitting false 423__843__6700___04_03__09 SIGNATURE OF PRINCIPAL EXECUTIVEI WIfoteation. kinding te pssilityof fine and Imprisonment for kinowing tv aions. OFFICER OR AUTHORIZED AGENT. AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIO-LATIONS (Reference afn atachments here)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199) Pretvous edit ons maybe used Page 1 of I
LLmUIIIJ SrbItM Svb (NPDES) MAJOR IForm Approved.
lItfame l=At t.UUYAH NUCLtEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Addrss P. OX 2000 __ _
I TN0026450 118 G -FINAL SDDY -DAISY TN 37384 I PERMIT NUMBER DSCHARGE NUMBER WASTEWATER & STORM WATER adcjl__D A. SEOUOVAH NUCLEAR PLANT_
Location HAMILTON COLUNL I - tONI1LQRiNG ERIp 8EFFLUENT ATTN: Stephanie A. Howard YE R I Mg I DA_
From 04 l 02 l 011 To 04 l 02 l 29 _I **
YEAR MO NO DISCHARGE [E ^^
NOTE: Read Insirucions before completing this form.
I NAME/TITLE PRINCIPAL EXECUTIVE OFFICER iI Certifyuntderpenatty of low that INsdocument and ell attactments were prepared unider my l
- -- -- - - - --- - - - - ---- A- - .-- - - --.- - .- _.
W.0 Richard T. Purcell iproperly gather amide~alate thle Wom ion submitted. Based on my kIqufry of he person or persons who mnage the systemr or those persons directly responsible for gathering the Information. the Wormation submitted is . to the best of my knowledge and betief, true. Prlncipal Environmental Engineer Site Vice President accrate enad complete. I am ware that there are stgnificant penalties for submitting false ihomiatlor indutding the possibility of fine and mpxisoenment for knowing vioitatons. SIGNATURE OF PRINCIPAL EXECUTIVI OFFICER OR AUTHORIZED AGENT TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attchments here)
During this reporting period, there has been no f1ow from the Dredge Pond other than that resulting from rainfall.
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1.