ML033360255
| ML033360255 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 11/11/2003 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML033360255 (105) | |
Text
Tennessee Valley Authority. Post Office Box 2000. Soddy-Daisy, Tennessee 37384-2000 November 11, 2003 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6* Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR OCTOBER 2003 Enclosed is the October 2003 Discharge Monitoring Report for Sequoyah Nuclear Plant.
Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission 1o-)
ATTN: Document Control Desk Washington, D.C. 20555 Peteej amp FF>si Her
PERMITTEE NAMEIADDRESS Include Facility Namekocation ifDifferent)
Name TVA -SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ____________
_ _ __fLNTENOFFIE B-_____
SODDY DAISY TN 37384 facjlity TVA. SEQUOYAH NUCLEAR PLANT Location HAMILON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPOES1 MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
Form Approved.
OMB No. 2040-0004 TN0026450 PFRPMITNIIARF:R I
101G F-FINAL
]
DISCHARGE NUMBER l DIFFUSER DISCHARGE NG PERIOD 1 EFFLUENT l
l I YEAR ATTN: Stephanie A. Howard From t03 1 10.I l
I l
I l
I l
I l
NO DISCHARGE E
l 1 01 j TO 1 03 1 10 I NOTE: Read nstrudions before comoletina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
SAMPLE 24.6 04 0
31131 MODELD CENTIGRADE MEASUREMENT 00010 Z
0 0
.. lPRMIT
- =***'*.
Z.
- C**** **-
- h*
30.5 D
DEG.C.
SEE CKREQ REQUIREMENT'
<..~
DIYM INSTREAM MONITORING TEMPERATURE, WATER DEG..
SAMPLE 37.7 04 0
31 131 RCORDR CENTIGRADE MEASUREMENT 0010 1
0 0
-PERMIT.-'>
- ,RP T.DEG. C.
SEE
.CK REQ EFFLUENT GROSS VALUE RERIRE
...*M A
PERMIT PH SAMPLE 7.1 7.4 12 0
10/ 31 GRAB MEASUREMENT 00400 1
0 0
PERMIT g
SU WEEKLY GRAB EFFLUENT GROSS VALUE REQUIREMENT a
- a
~~~~~~~~~~~~~MINIMUM"'~
- .w-AXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE
- 6 8
19 0
5131 GRAB MEASUREMENT 0530 1
0 0
PERMIT~
,-.*i**~'WEL GRAB EFFLUENT GROSS VALUE REQUIREMENT.
3 O A DL MGIL
~~~~~~~
~~~~~MO AVG':
DAILY MX`-
OIL AND GREASE SAMPLE
<5 19 0
5/31 GRAB MEASUREMENT 00556 1
0 0
.'PERMIT,,,,,*
20 MGIL WEEKLY GRAB EFFLUENT GROSS VALUE RQIEET OAG DIYM FLOW, IN CONDUIT OR THRU SAMPLE 1590 03 0
31131 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 0
PERMIT...
REPORT-MD,.
~**
CONTIN RCORDR EFFLUENT GROSS VALUE PEMT -e D
.X
,U CHLORINE, TOTAL RESIDUAL SAMPLE
<0.012 0.018 19 0
32131 GRAB MEASUREMENT
<.1
.1 9
0 311 GA 50060 1
0 0
PERMIT:
0.036 0.058 MGQL WEEK-CALCTD EFFLUENT GROSS VALUE REQUIREMENT iNS M AYS NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify nder penally of law that this document end all attacments were prepared under my direction or swervision in accordance with a system desioned to assure that oualfied ersonnel Richard T. Purcell propeny gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system or those persons directly responsible for gathering the inormation. the Wormation submitted is, to the best of my knowledge and belief, true accurate and complete. I em aware that there are significant penalties for submitting false hformation including the possibility of fine and Imprlsonment for knowing violations.
Acting Environmental Supervisor SIGNATURE OF PRINCIPAL EXECUTME OFFICER OR AUTHORIZED AGENT Site Vice President 11 TYPED OR PRINTED I. _______________________________________________
I.
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference al attachments here)
No closed mode operation. The following Injections occured: 1. PCL-222 (max. calc. conc. was 0.058mg/L-limit 0.100mglL) 2. CL-363 (max. calc. conc. was 0009mg/L-limit 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.025mg/L-limit 0.100mg/L) 4. H-130M (max. calc. conc. was 0025mgIL-limit 0.050mg/L) 5. H-130M (low detection level analytical method was 0.046mg/L-limit 0.050mg/L))
EPA Form 3320-1 (REV 3199)
Previous editions may be used Paae 1 of 2
PERMITTEE NAMEIADDRESS (Include Facilth Name4ocation ifDiferent)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000
_LN E~ROFFICE S1BLA----------_
SODDY DAISY TN 37384 Faait_ WV -SEOUOYAH NUCLEARPLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES)
MAJOR DISCHARGE MONITORING REPORT (OMRJ (SUBR01)
TN0026450 101 G F-FINAL PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE' Form Approved.
OMB No. 2040-0004 I
MONITO)PING PIlD I
EFFLUENT From L 03 1 1 0]To 03 1 0 j 3
ATTN: Stephanie A. Howard
" NO DISCHARGE W
NOTE: Read Instructions before comoletina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
_FREUENC SAMPLE
~~~
~~~
~~~~~~~~~EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE e**
- 0.
0 31 /31 CALCTD CHANGE M
MEASUREMENT 62 82234 1
0 0
.;'., 'PERMIT
- wx(*e*e,~
~
DG OTNCLT EFFLUENT GROSS VALUE
- RERM z
IHR u;;
D_
TEMP. DIFF. BETWEEN SAMP. &
SAMPLE
- 2.
4 0
31/31 CALCTD UPSTRM DEG.C EASUREMENT 00016 1
S 0
PERMIT,.
- ^****
. ;- ****eee*-.
- eeeeee*
3.0.
DEG.C.
.' CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT UOUS BORON, TOTAL SAMPLE
<0.2
<02 '<02 19 0
1/31 GRAB MEASUREMENT 19 01022 1
0 0
-EU ITRE T
RE T
,REPO T REPORT M
NE G
REQUIREMENT EFFLUENT GROSS VALUE SAMPLE MEASUREMENT
-PERMIT..
SAMPLE MEASUREMENT.
BEQUIREMENT SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT
`PERMIT-_,-
REQUIREMENT'-
NAMEtTITLE PRINCIPAL EXECUTIVE OFFICER I Certifyunder penaltyof law that s document and aln tahnrnents were prepared under my i
l TELEPHONE DATE ireclion or supervision in accordance with a system designed to assure that qualified personnel TELEPHONE DAT 1 n.1AI Richard T. Purcell property gather and evaluate the information submitted Based on my inquiry of the person or L
Persons who manage the System. Or those Persons directy responsible for gathering the Acting Environmental Supervisor 423 843-6700 03 11 13 Site Vice Presidentoration th ~inforaion sumtdi.to the bes of my knowledge and belief. true, 423___843__6700__03_________3 Site ice President acourate, and corpete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, Including the possibility of fine end iprsorment for knowing violation, OFFICER OR AUTHORIZED AGENT CODE NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS Reference afl atachments here)
CCW data for October 2003 is attached.
FD~~~~i F^lqe,0 eaosn ma oe~
useoA Dg~o 2 ".AO~
xr rr rrM J.u-lCV 3rYY}
Previous editions may be used Page 2 of 2
CCW CHANNEL DaterTime Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 1012012003 0812
<0.5 mg/L 10/23/2003 2306 JBR EPA 8015B CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis DatelTime Analyst Method 10/20/2003 0815
<0.5 mg/L 1012312003 2344 JBR EPA 8015B
PERMITTEE NAME/ADDRESS (Include Facilitv Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT -
Address P.O. BOX 2000
_LN EfROFFICE LB -
_____fOD D AISY_
T 37384 Failitr_ TVA -SEOUOYAH NUCLEAR PLANT___
Location HAMILT CO_ UNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040-0004 l
TN0026450 F101 T
l
-FINAL I
PERMIT NUMBER I DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 AANITOPIMtl PFRlfOD I
EFFLUENT YEAR MO FoDAY YEAR MO DAY From 03 lo 01 To 03 1 0 31 ATTN: Stephanie A. Howard NO DISCHARGE F
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE
>100 23 0
1/90 COMPOS CERIODAPHNIA MEASUREMENT TP3B I
0 0
<.PERMIT.,
2~
e***
49~.m'***
PER CENT QUR-CM S
EFFLUENT GROSS VALUE RE RME
-MINIMUM
.R P_
IC25 STATRE 7DAY CHR SAMPLE
>100 23 0
1 /90 COMPOS PIMEPHALES MEASUREMENT TRP6C 1
0 0
PERMIT.
PERCENT QUART-COMPOS ERREQUIREMENTE REOU I
E E.
-MMINUM SAMPLE MEASUREMENT
-- PERM T.
REQUIREMENT;-
w.:,.K4 SAMPLE MEASUREMENT PERMIT REQUIREMENT.,
IM SAMPLE MEASUREMENT PERMT.:,-
5 f.
REQUIREMENT' SAMPLE MEASUREMENT
. PERMIT'-
REQUIREMENT SAMPLE MEASUREMENT
~REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my 1
/
I TELEPHONE DATE firection or supervision in accordance with a system designed to assure that qualified persorre fl^
$tit M g LI)
T Richard T. Purcell properly gather aend evaluate the information submitted. Based on my kinusry of the person or Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE L.
TYPED OR PRINTED information. cludi0 the posibility o fine and mprisonient for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ettachments here)
Toxicity was sampled October 5 10, 2003. Report Is attached.
_or 30.1 (RE
- 31.
Prviu edtin ma be................
use Pa.
1 of 1...............
EPA Form 3320.1 (REV 3199)
Previous editions may be used Page of 1
November 6, 2003 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, H130M SPECIAL STUDY, OCTOBER, 2003 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected October 5-10, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB IB-M SQN Hl3OM-October 2003m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: November 6.2003
- 1. Facility / Discharger:
Sequovah Nuclear Plant / TVA
- 2. County / State:
Hamilton / Tennessee
- 3. NPDES Permit #: TN 0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 3266
- 6. Receiving Stream:Tennessee River (TRM 483.6)
- 7.
1Q10:
2992.4
- 8. Outfall Tested:
101
- 9. Dates Sampled: October 5-10, 2003
- 10. Average Flow on Days Sampled (MGD): 1582, 1584,1585
- 11. Pertinent Site Conditions:
H130M was injected from October 5-10, 2003. The dates and times for the H130M injection are in the following table.
Injection Locatin
.Date/Start Time Date/Ending Time Injection Location (EDT)
(EDT)
Essential Raw Cooling Water (ERCW) 10-6-2003/1005 10-7-2003/1004 B T rain Raw Cooling Water (RCW) 10-8-2003/1000 10-9-2003/0959
- 12. Test Dates:
October 7 - 14, 2003
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pirnephales promelas)
Daphnids.(Ceriodaphnia dubia)
- N
- 15. Concentrations Tested (o):
Pimephales promelas:
- 16. Permit Limit Endpoint (%):
Outfall 101: 10.98. 22. 43.9. 72, 100 Intake: 100 UV treated Outfall 101: 10.98. 22, 43.9. 72. 100 UV treated Intake: 100 Outfall 101: ICs = 43.9%
- 17. Test Results:
Outfall 101: Pimephales promelas: ICZ> 100 Ceriodaphnia dubia: IC 5> 100 UV treated Outfall 101: Pimephales promelas: IC 5 > 1 00
- 18. Facility
Contact:
Phone #:
Ruth Ann Hurt (423) 843-6714
- 19. Consulting / Testing Lab: Environmental Testing Solutions. Inc.
- 20. Lab
Contact:
Phone #:
- 21. TVA
Contact:
Phone #:
Jim Sumner (828) 350-9364 Cynthia L. Russell (256) 386-2755
- 22. Notes: Outfall 101 samples collected October 5-10, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
2
METHODS
SUMMARY
Samples:
- 1.
Sampling Point: Outfall 101. Intake
- 2.
Sample Type: Composite
- 3.
Sample Information:
Date (
Date Arrival tial Date Sample (MMJDDJYY/
(vMMJDD/Y)/
Tempal
'Inta (MDD/YY)/
ID Time (EDT)
Time (EDT)
Tep R*
Time (EDT)
ID__Tim Collected Received (eC)
(mg/L)
Used 10/05/03 1357 to 10/07/03 1245 101 10/06/03 1257 10/07/03 0942 0.8t
<0.10 10/08/03 1231 Intake 10/05/03 1413 to 10/07/03 0942 0.9
<0.10 10/07/03 1245 10/0/03 1330 10/09/03 1233 101 10/08/03 1230 10/09/03 1000 1.1
<0.10 10/10/03 1235 10/0/03 137t 10/10/03 1233 Intake 10/0/03 147 o 10/09/03 1000 0.8
<0.10 10/09/03 1235 10/09103 1305 to 1.3
~~~10/11/03 1219 101 10/09/03 1305to 10/10/03 1931 1.3
<0.10 10/12/03 1228 10/10/03 1205 1.9
~~~~~10/13/03 1231 10/09/03 1320 to 10/11/03 1219 Intake 10/10/03 1220' 10/10/03 1931 2.1
<0.10 10/12/03 1228 10/13/03 1231
- TRC = Total Residual Chlorine tCollected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
- 4. Sample Manipulation:
Samples from Outfall 101 and the Intake were warmed to test temperature (25.0 +/- L.0C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes.
3
Pimephales promelas Ceriodaphnia dbia Test Organisms:
- 1. Source:
Aquatic BioSystems, Inc.
In-house Cultures
- 2. Age:
20.75 - 22.25 -hours old
< 24-hours old Test Method Summary:
- 1. Test Conditions:
Static. Renewal Static, Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10 10 I
- 6. Test Initiation: (Date/Time)
Outfall 101:
UV Treated Outfall 101:
10/07/03-1245 EDT 10/07/03-1232 EDT
.10/07/03-1212 EDT
- 7. Test Termination: (Date/Time)
Outfall 101:
10/14/03-1226 EDT UV Treated Outfall 101:
10/14/03-1244 EDT 10/14/03-1200 EDT
- 8. Test Temperature: Outfall 101:
Mean=25.10C (24.6-25.60C)
Test Temperature: UV-Treated Outfall 101:
Mean = 25.10C (24.6-25.50C)
Mean = 25.1 C (24.6-25.70C)
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxvgen.
- 10. Statistics:
Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software. McKinneyville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted October 7-14, 2003 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Intake 100 100 100 100 98 98 98 Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2
3 4
Mean Control 1.120 1.016 1.105 1.029 1.067 10.98%
1.123 1.166 1.084 1.156
.1.132 22%
1.240 1.105 1.169 1.206 1.180 43.9%
1.233 1.071 1.060 1.193 1.139 72%
1.165 0.951 1.219 1.258 1.148 100%
1.236 1.120 1.143 1.206 1.176 Intake 1.142 1.1 4 1.100 1.212 1.147 IC2 5 Value: > 00%
Permit Limit: 43.9 Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Loe Lii:N Permit Limit: 2.3 TUc LowerTLimit: NA
- TUa = I 00/LC50: TUc = I100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted October 7-14. 2003 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(%Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 34 30 34 31 33 35 30 32 33 35 32.7 10.98%
39 38 33 37 33 36 35 32 36 31 35.0 22%
39 32 35 34 35 37 34 36 35 32 34.9 43.9%
42 40 34 34 37 35 34 39 35 35 36.5 72%
40 37 37 38 33 38 38 41 35 38 37.5 100%
42 34 35 35 38 42 38 39 34 40 37.7 IC 25 Value: > 00%
Permit Limit: 43.9 Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Permit Limit: 2.3 TUc Lower Limit: NA
- TUa = 100/LC5o: TUc = 100/ IC25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 3.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted October 7-14. 2003 using water collected from Intake.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 l 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)
(% Effluent)
Daa replicate number) 1 l2 l3 l4 l5 l6 7
8 9
10 Mean Control 38 30 29 37 39 38 30 32 34 31 33.8 Intake 40 40 38 32 37 36 36 32 39 36 36.6 No significant difference based on Homoscedastic t-Test 7
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 4.
Results of a Pimephales promelas Chronic 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted October 7 - 14,2003 using effluent from Outfall 101 (UV Treated).
Test Percent Surviving Solutions
_ (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 98 Test Solutions Mean Dry Weight (mg)
(% Effluent) 1 2
(replicate number)
(% Effluent)
I l
2 3
4 Mean Control 1.069 1.156 1.173 1.233 1.157 10.98%
1.093 1.199 1.144 1.088 1.131 22%
1.179 1.105 0.930 1.193 1.102 43.9%
1.156 1.216 1.197 1.107 1.169 72%
1.009 1.137 1.312 1.094 1.138 100%
1.241 1.266 1.216 1.233 1.239 Intake 1.225 1.197 1.035 1.221 1.169 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Permit Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25)
Pirnephales promelas October 7, 2003 1306 7-days KCI 614.8 mg/L I Ceriodaphnia dubia I October 7, 2003 1 1150 7-days I
NaCl I 1020.4 mg/L I 8
6 9SZ wnwixeiv' 9'UZ untulully 1 *5r I *SZ vDJqnp vpyudvptuaj sviatuaOd s57/ydaqdJ b0) ainevaduuw llgJaAo 01'0>
01'0>
010 >
IL 69 t9 Z9
£9 6LI -
L91 99-L -
S'L 8S'L ZL OLI 19L t'L 6-L -
SL L'L
£ L 08 ZrS
- 6 SSZ - 9'VZ l'SZ l'SZ OL ancJuI 01'0>
- 01'0>
IL 69 99 Z9 SLI -
891 99L -
L 65'L
£'VL 6-L - 9L V8 -
9'L Z'S
- 6Z SSz-9tVZ 0o>
£9 ZL 09'L 91L 8'L 08 I'SZ 15Z 001
. £1Z -
£ 0
Z LI
- SOS L9L - 55L 09
'L O£-
9L 8 -
9L Z'Z - 6Z 5S5
- 9Z l ZL 90Z Z9'L S'L 8L O'8 ISZ l
I'SZ SVZ -
S£Z
£LL - 85L 179L - ZS'L OS -
-L
£'S
- 9L ZrS7 - 6Z S SZ -
z Ot' b79L LS-L S'L 08 l
5Z l
I5Z
/_6___
SLZ -
6SZ LL'L - LS'L 99'L - SS'L O'8 -
SL VS -
9'L ZS
- 6 S5
- 9Z ZZ 99Z S9-L Z9'L SL O'S I-5Z I-SZ 98Z -
9L
£S -
91 ZSZ - 6Z SS86 01 LLZ 99'L t19L 8'L O-I-SZ I-SZ r)R.
Q rl Z,
rz-11 t-
Z, 98 VS 58 19 09 09 CiE1£ LSI IS-L - LS'L I1-L - S'L t6Z L9L IL-L VS S'L S'L VS -
L'L 6L Z'SZ - 61Z I SSZ - 9'bZ l'SZ l'Sz IoJ)UOO 01'0>
01'0>
01'0>
IL 69 OL t'9 -
Z9
£9 6LI -
Z'rL 6L -
Z 9 O£'L, t'L Z'L
£-S -
'L 08 Z s
- L 9 5
- ctvz O SZ
£'SZ at3uIU Ol'O>
- O'O>
IL 69 99 Z9 8LI -
891 IS'L - SOL 6S'L
' L 6-
£9 VS
- 91 ZSZ -
07Z 95Z - L'Z
%001 O'O>
£9 ZLI O'L 9P'L ZL O-S OSZ
£'SZ
£1
£OZ IS'L - LOL Z9L -
W'L SL -
£9
£S - 9L
'S
- Lt'Z 9'5
- 9tZ
- ZL 90Z 6Z-L ZS-L I-L O'S O-SZ Z-SZ st'r -
S£Z IS'L - 90'L tr9L r S'L 6L -
Z9 V S 9'L ZSZ - 417Z 9*S
- 9Z
£-S -
9'L
'SZ -
tZ 9'S
- 9
- /ZZ
£9Z 6Z'L Z9'L I'L O'S OSZ Z'SZ 95Z S9r
£S'L - S0'L VL'L - LS' 6L. -
6'S VS - 91, L
ZSZ - 0tZ 9'S
- 9 8
LLZ I£ L b9-L Z-L OS O SZ Z'SZ
%S6'01 la A
Q E
lt3 61 ii11 a
98 VS 19 09 09
£1£ -
LSZ V6Z rS'L -
IIL
£'L 11 - OS'L OL-L 08 -
19 Z-L
£-S -
LL 6'L ZrSZ - L'r 9
- 9 Z O'SZ Z'SZ oJ)Uoz) -
(Tj~w) auipolqa (roa:)uo OD) eozV: 01ju) (wapsoqwdi) 1 I8U"I luillul
- jUuJ IB Ii1 jBjU!
iBImu 1
ienpisaU 1M1ol ssaupJell IHUIIIIV Ia3u3npuooJ
(-n-s lid I(q/3 uJ.
NAX0 PalOSSJU
- 0) aJnhJcadwl, Il a iadweS I sjs 00Z 'it -L Jaqop
'101 IlljlnO (NbS) luonj iudIdiclPN 4qiAonbS 'sisa5 v!qnpktuqdvpoi.ai pue sawoJdsalrydawnud 1oj saSue) pue sanleA ueNails watj3luM AHVIVI1VlS 'IV II'IlDIVJISAId
PIIYSICAL/CIIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pinephales prornelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, October 7 - 14, 2003.
Test Sample ID Temperature (0C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Initial Final Initial Final Initial Final (pmhos/cm)
Control 25.3 24.9 8.0 7.3 7.60 7.34 286 24.6 -
25.7 24.7 - 25.1 7.8 8.4 6.3 8.2 7.52 - 7.66 7.05 - 7.60 277 -
295 10.98%
25.3 24.9 8.1 7.2 7.61 7.33 279 24.6. - 25.7 24.7 - 25.1 7.9 8.4 6.2 8.1 7.53 -
7.68 7.05 - 7.55 274 -
290 22° 25.3 24.9 8.1 7.3 7.59 7.34 266 hiO______
24.6 - 25.7 24.7 -
25.1 7.8 8.4 6.3 8.1 7.51 - 7.67 7.10 - 7.57 262 -
276 43.9°/
25.3 24.9 8.1 7.3 7.58 7.32 240 E. _____
24.6 - 25.7 24.7 -
25.1 7.8 8.4 6.3 8.1 7.47 -
7.67 7.09 - 7.54 232 -
249 72%
25.3 24.9 8.0 7.2 7.54 7.32 208 24.6 -
25.7 24.7 - 25.1 7.8 8.3 5.9 8.2 7.41 -
7.64 7.04 - 7.55 203 215 100%
25.3 24.9 8.0 7.2 7.51 7.30 172 24.6 -
25.7 24.7 - 25.1 7.8 8.3 5.9 8.2 7.35 -
7.63 7.00 - 7.53 168 -
179 Intake 25.3 24.9 8.1 7.2 7.48 7.30 171 24.6 - 25.7 24.7 - 25.1 7.8 8.4 6.1 8.0 7.31 - 7.62 7.02 -
7.52 167 -
177 Overall temperature (C)
Pimnep hales proinelas Average 25.1 Minimum 24.6 Maximum 25.7 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected October 5 - 10, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1.
Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers' each containing 250-mL of test solution.
- 2.
Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL I.
Pimephales promelas None
- 2.
Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1.
Pimephales promnelas None
- 2.
Ceriodaphnia dubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Total Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCI.
- 2.
Standard Toxicant:
Potassium Chloride (KCI crystalline) for Pirnephalespronelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN 0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
14
Sequoyah Nuclear Plant Biomonitoring October 7 - 14, 2003 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, 15
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-October 10, 2003 Date
`Sarodiuhi--
Towerbrom ',PCLU222---
PCL-401
- CL;363, Cuprostat-H130M
¢-Hyochlonte mg/L
- -'Am mgL
-mg/L; PF g/U TRC
£Pho~phate Copolymer D ADl fL Qua 03/12/1998 V
.0i6 S
1'-'
03/13/1998 3q 015.
03/14/1998 iV6Y0.O3,;-
03/15/1998 0 0.03.,
03/16/1998
-0.013-
-~.-
03/7198
"~:0.O21-*~.
09/08/1998 ' -
0.0 0.014 0.005
'.0.0217, 09/09/1998
,-.% ;0.003,
- 0031 0.011 09/10/1998
..- 0014 0.060 0.021 09/11/1998 0.o.3
.055'?'
0.019 09/12/1998
<o o
i 044.-
0.015 09/13/1998 r
< 0.001 0044 0.015 09/14/1998
- 0
' i
.0044 0.015 02/22/1999 0.001 02/23/1999
. 0.0.
-5 02/24/1999
, 0.009 02/25/1999 0.012:.
02126/1999
- .0.008 02/27/1999
.< 0.001 -
02/28/1999 -
<0.001 s
08/18/1999 -.
0.015
.- 0.069 {:..
0.024 0.006 08/19/1999 0.012
- -: 0.068 0.024 08/20/1999 0.023
-0.070 0.024 0.120 08/21/1999 0.022
- 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0024 0006 08/24/1999 0.016
-0.067 0.023 0.020 16
Table B-i (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-October 10, 2003 Date Sodiuiim%4 PCL-401
'CLX2363Towerbrom.
01' Cuprostat-H-j30M m~~~~~~~~~mg/L gX;,PF mg~n~fi~g TRC
+PTh8 haiti, Copolymer iD MDf mgtL
- ,jQuat t.itt'.-RC'4 Z
'- '&4 ' ^ssA;3 #';. gu.Azole 01/31/2000 02101/2000
'Az 0.011 0 02 6,20-028 02/0212000
- 0.
<.028026 0.009
- 0 006.
02/03/2000 2_ >
0.008 0b.027,--
0.009
'Vr.
02/04/2000 00 5
- 05006 0275K 0.009 0 5.,
0.109 02/05/2000
<0 0002
,50027.-
0.009 02/06/2000
- 0.
<0 002 i
- O.o 27 0.009 07/26/2000 7<'
0'
<00057
<0.055 0.019 7--
07/27/2000.-
0.019
- .>0.O0551`~~-
0.019 07/28/2000 0.0088
- 2. 0.053 0.018 0 04 0.108 07/29/2000
< 00088
-9.O055 0.019 07/30/2000
< 00076 0.055 0.019 07/31/2000 1
< 00152 "0.055 0.019 0.006 08/01/2000
< 00141 ;
.O5S 0019 O-O 12/11/2000 0.0143 Y'0 025 0.020 0.005 12/12/2000 r
r 00092 *O.025 0.020 OO:,.
12/13/2000
<00120 0.025..
0.020 12/14/2000
< 00087 0.0.025 C.
0.020 12/15/2000 i000120
- 0.025 0.020 0005 s
12/16/2000 -§
<00036
`, 0.025,-
0.020 12/17/2000
< 00036 0.025 --
0.020 08/26/2001 0.017
- -0.06 0.021 0.006 '
08/27/2001
<0.0096
- '. 0.06 I
08/28/2001
<0.0085 06 0.021 00 0
08/29/2001
<0.0094 0.059,;.
0.020 ~
-0.005 0 021 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001 __r,
<0.005 0.059 0.020 11/25/2001-.
<0.0044 1:.-'-
I 11/26/2001 ',
<0.0119 0.024 -
0.02
- 0.005 11/27/2001 _ -
0.0137 l
0.O23 l
0.019 l0007 l 11/28/2001
<0.0089 60.022' 0.019 0.006 11/29/2001 r
0.0132
- 0024 0.02 0.007 11/30/2001
<0.0043 7.0.024 0.02 12/09/2001
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 i
12/12/2001 0.0128
-0024' 0.02
.008 12/13/2001
<0 0088 6-'0 02, 0.02 12/14/2001
__________l 0.0134 0024 002 0.007l 17
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-October 10, 2003 Date
~Sodiiic~j4 Towerbrom PCL20 1C C1 6 Cuprostat-
.TRC Copolye D
rn/C opolymer i
01102/2002
< 0.0079
'*,0.023 0.02
>V0.006 01/03/2002 ~
0.0042
-rO 023Wi-0.014 01/04/2002 0.0124
,. 0O24,
0.014
_,09 01/05/00<
~
<00042 01/06/2002 SC' 2
<0.0042 01/07/2002
< 0.0089 0024-0.014 0006 02/24/2002
<0004 I,
02/25/2002,,r
' r
< 0004 0 023 0.023 02.26/2002 0.0143 O,O 0.023 0.007 02/27/2002
.<0.0041
,0023 ^
0.023 02/28/2002
< 0.0041
- '!02O 4 0.008 o
03/01/2002
<00041 0.024 '
0.008 05/05/2002 05/06/2002 tK*
0.058 -
0.02 0014 05/07/2002
-. 0.058
^
0.02
- 0.015 O
05/08/2002,
0.056 0.019 TV 05/09/2002
- '.0.05-2 0.02 0014 05/10/2002 t
...0.056'<
0.019 08/04/2002
<0.0058
- .j-'
08/05/2002 K
<0.0058
- 0.053 0.018
- l0025, 08/06/2002 00092
-0.053 0.018 08/07/2002
<0.0107
' -. 0.055';
0.019 0 007 08/08/2002
<0.0061
- 0.055 0.019 08/09/2002 0.0152 0;054 0.018 0008 l
10/06/2002
<0.00497 10/07/2002 0.0153
-0.054
0.018 0009 10/08/20021
<0s-s'-
002 0.054 0.018 0.007 10/09/2002 001 0.03 0.01' 0.009 10/10/2002 0.0134
'0.054 0.018 0.009 10/11/2002
<0.0042
.- 0.054 0.018 01/12/2003 Th S
<00035 01/13/2003
<0006 0.025 0.019 0.009 01/14/2003
<0.0118
, 0.026 0.020 01/15/2003
<0.0063 0.026 0.020 0.009 '
01/16/2003
<0.0034
0.026 0.020 01/17/2003
?,
'..l
<0.0034 l
0.026 0.009 l
04/06/2003
<0.0073 04/07/2003 l
<0.0189 0.021 04/08/2003 '
<0.0117 0.021 04/09/2003
<0.0139 l
0.021 10.06 04/10/2003
<0.0 1 13
- l.
0.021
--0.0,8 04/11/2003
<0.0073 0.022 18
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-October 10, 2003 Date Towerbrom PCL-401 C
6 Cuprostat-H:130M C11~~lo~t~)
mgfL ~ gtŽ2 mg/L ngl PF-I,fk TRC 1JPhos aiz Copolymer iDMmg coat' ~
DA" mgAL XY~uati
_________<<)
z2 Azole 06/15/2003 c 9o `-'o7
'3
< 0.0045 e
06/16/2003 r
<0.0037 0-0 0.020 00&
06/17/2003
<ir-0241"i
<0 0048 20.041i, ~
0.014
~
2~
06/18/2003
< 00048 2T0412 0.014 "O
.,t.024, 0611912003 ->g v; -^ ;^s ^
< 00085 z
A40.058 sJ 0.020,
-0.025 06/20/2003
< 00048 0
8' 0.020 r0 0252 08/03/2003
<00050 08/04/2003 0
<0.0050 0058 0.020
-s
'- -z 08/05/2003 r
<0.0051 0:0o7 0.020 0.025 :
08/06/2003 6 _ -
<00084
,O.057: :u 0.020 0.025 e 08/07/2003
-.i.i0129
.057 0.020 0 024 08/08/2003 0.0153 0057-0020 0009 10/05/2003
<0.0043 0.057 0.020 10/06/2003 A-s'--
<0.0043 00 057 0020 0 025 10/07/2003 e
<0 0090
.-0.057 00 0.025-10/08/2003
<0.0106 i
0057 0.020
'0.025.
10/09/2003 00181 0
.06 0.022 0025 t 10/10/2003 0.0183 00260.024 0.009 19
Sequoyah Nuclear Plant Biomonitoring October 7 - 14, 2003 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets 20
BIONIONITORING CHAIN OF CUSTODY RECORD Page _1_ of U Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Scquoyah NP NPDES Number: TN(02645()
Collected By: Wanda Allen, Ronnie lankins Environimental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
G~~)
UPS Bus Other (specif):
Client General Comments:
- C6d, kaL LJ~
,AAM&e I'A Fax:
828-350-9368 Container s
4 N
Field Identification /
r C
Number &
Flow X
v9 l1 Grub/Comp. ~~~Collectioll Daterrinle Ra vn
>X*
x.; I.X~
t0X9>x~z>X~x~~a<~~oX X:XXXXfX; Sample Description ou (G
):.xx VapeDecltll olumetctl (MCD)
(Mark as Appropriate)
.X, X
.Daw
'lim Q
Ycs 1ff No Trace m
XX: xAivatTomp r y
Tameu IEM Tm pcX
~
~
OR "tndi~es
'Nme 1015/03 1016/03 SQN-101-TOX Comp
,5y I
2(2.5gal)
NA
/
6e*1 j%
i SQN-INT-TOX Coip I()5/()3 1(13 1(2.5 gal)
NA A
.r
._____________________ Sample Custody - Fill In From Top Down Relinquished By (Signattirc):
Date/Tiic Received By (Signature):
Datefrime Ronnie Hankins p
10/6/2003 FedEx 10/6/2003 FedEx 0
q//-
tnn lo o -O103 0§Jz
_____________________1-Cf-03 Instructions: Clients should ill in all areas except those in the "Laboraltory Use" block. Biomouiitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time or collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via redEx or UPS must be matked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page_-2_ of_-3_
U.
q Client: TVA Project Name: Sequoyah NP Toxicity P.O. Number: N/A Facility Sampled: Scfloyali NP Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
UPS Bus Client Other (specify):_
General Comments t
4c NPDES Number: TNl026450
.Collccted By: Wanda Allen, Ronnie liankins "ACLf LdeGa d Co
&.to L050 cwk-~
Fax:
828-350-9368 I
- ^'
XXV'-:.:Y.::',:::X::>.X'.:-.>.y.S'XX'.'-,'X-.'.S:X-.8..i>XXX:eXYX-.'.X::::X:;.::;iS.:X:>.
.>:Yx::xxxxxxxxxxRxxxxxxRXxxR'xxxxxxxXXxx'x:<;ixRxxxxxxxx:ox::x::xx.
SXX::X::XR*X:4x9XX>'XXXX.9XR.xx.XX*XXX$:RXXx.fXXXxxXXSXXXXXRa:.S:Xx4
,.:fi-
- {:XX:iXXXXX::XX}:XX:.'X.:<.'.:XXXXXXXXXRXXXRX4X#X#XXX.v.}:'RXXXXXX:eXXX::XX':
Ux:.xxxx::::xRxxxx.xxxxxRxX:X.x'RxxxxRXxxxxxxxRxxxxxxxxYxSy: ::xt::x
- >:X:eyXXXXCXXXXX<Xy<t9:#Y.iXRS#XXXXX'SeX+vRXR-.eXXXhXXXXXXyXXXX
- XXX:
- R: ::X:.:;.:;:x:c:
xxxt:'>'xX>.XXXP: f:+w-X'.;X'}
gR'x;-> (XRXX#XXXSXR#X;XXXXXXXS:>X
- x.:x:eXxxxx>:xx;RexRxxx;ix; r4 f T I:xxxx>:xxxxxxxxxxxxxxx::
- .n
'X::XXyXXXXXXR:Xy..>XX:.,_w!_
- Yt-'XxRR2xxxx#xS2Sxxxx::xx rees X r RfiXx x g x Y x SKRX XX
- X XX#X X x x
>i#x;^ x x >x S X XX X x x X x x x a
I Rclinqtmished By (Signaturc):
Date/Timne Received By (Signature):
Date/rime Ronnie Ilankins 10
.8 10/82)3 FedEx 101812003 443 10/8/2003 FedEx is.~~~s, Fed~~~x 4W~~~aWLLI-R m-oci-n 1=1 ~~~~~~~~~~
)O-OR-0,14 KM~~~~~
,~~~~~
Instructions: Clients should ill in all areas except those in the "Laboratory Use" block. Biomnonitoring samples are preserved by storing them at 6C and shipping them in ice.
he hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Thereore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Iriday via FedEx or lIPS must le iaiked br Saturday delivery or hey will jiot arrive until the IbIlowing Monday.
I a
BIONIONITORING CHAIN OF CUSTODY RECORD Page _1_ of Client: TVA Project Name: Sequoyalh NP Toxicity P.O. Number: N/A Facility Sampled: Sequoyah NP NPDES Number: TN0026450 Collected By: Wanda Allen, Ronnie lankins Environmental Testing Solution, Inc.
351 Depot Street.
Asheville, NC 28801 Phone:
828-350-9364 Delivered By (Circle One):
QED UPS Bus Client Other (specify):
General Comments:
ClMU oCt suif kL0 du,6W cat0-Fax:
828-350-9368 Container l (MD l Field Identification Nbr&
Fo S:.x-,:
.rab/Comp.
Collection Date/Time Rain Event?
M I
m Sample Description Volume (MGD)
(a NAropriat) xet SQN-NT-TOX 1
Comp T,§2 rf>/ro.lD3 1(2.5 gal) l NA T-0 T
5 I
3 0u X zz(a0r a
p t
NgOSM Nxxx; Relinquish~ed By (Signature):
Date/Time Received By (Signature):
Daterrime Ronnie Hankins R.
4 10/10/2003
('1:3/
1O 10/10130
~~
l
~
"/(c/c', (g3/ss" T
~
,w b ot lI--03 931
.~~~~~~~~~~~:.
Instructions: Clients should ill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via Fedfix or UPS must he maiked or Saturday delivery or they will not arrive until te following Monday.
&90
-- m Environmental Testinp-Solutions. Inc.
Page I of 6 Environmental Testing Solutions. Inc.
Pare I of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TtVA Facility: Seauovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project H:
8fILI Diludon prea t information:
Comments:
Dilution PMe (.0)
Jlb.'?A 22 A
9 Effluend volume (mL)
Ay
____9__
Diluent volume (mL) tZ52
/b2.6
_B. 0 Total volume (mL)
Zoo 0
5SZc)
Test organism information:
Test information:
Organism age:
8.15 2. 2 -
JvaOLS Randomizing template:
698WeJI Date and times organisms I6-06--a 123o -M 1"400 Incubator number:
were born between:
b_
_3_C Organism source:
A >Si CE I6-Ac-C A. rtemia lot number:
B&0d13 Transfer bowl information:
pH =
Temperature =
C Total drying time:
JJ-
-I.lez
- -1 Date/Time in: 1O--!a I5 Average transfer volume:
- o. L Date / Time out:
S lIS.
pgbo Oven temperature:
100C-I I
I I
I Dailyfeeding and reneval information:
Day Date lMvlorning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding renewal, or batch used used time time termination time 0
to1 10(t VAs 10,D3 o
IC7.
z/.o a
I
~)oS-c3 o5l0 Is 0 zoz j-og-U3 n 1o-,
.oz-ai 2
lp-oqiG5 CR 0o iu5s is 7zO6 o
o-p 0,
I caq-z.O 04(
3 to-16-(13 2
l10 JQ-.t-Os a3 l
., z4.-
I 4
10-11 e 3
1 SM
.1ZIfD09-1 Pal.<.S 5
IDol 2a a5 10 ISloo-olO t.o(l*
6
/- 13-a3 o'2-ISCL IZ I 10-1O3
.0c3(a otI 7
Io-lQ.
ls-_3
__I o
cis Control information:
AcceptLancechteiia
[Summary of test endpoints:
% Mortalitv:
I 07 r
20%
j 7-day LCso l >
l Average weight per initial larvae:
I I. No1 -B NOEC I DO1%5 Average weight per surviving larvae: l. 0l-13 0.25 milarvae T LOEC l 7 Ot T ChV
> I tfl.
ChVs
>, i~ft.
- M iW' Environmental Testing Solutions, Inc.
Species: Pimephalespromela Client: TVA I Sequoyah Nuclea Non-treated z
Page 2 of 6 S
Date:
). 0 3
r Plant Survival and Growth Data F.
r Day CONTROL 10.98%
22%
A B
C D
E F
G H
I_
J K
L 0
I/
)b )//o0
/0 /c IL
/oO/6 /0/c
/D 16
/
I /0 /0
)0 /
/
/0
/
o
/1 2 1 1
/0
//
/0
/0/0 //
16
/
0 3
Lb
/, )
)
/0 D10 0
/
lbi-
/0 4 ~
l IC)
- D 1 to l I LI to lo
/D l loJ
/
__10 l
10 0
(0
/(J
/0 Jo to IV 0_
1
/0
)
C
/
1
/0 0 10 /0
/0
/0 6
/E/0//0
/0
/0
/
0
/0/
//O lb
_ /o I io
,o /o to,o A - Pan weight (mg) l b\\ I B-Pan + Larvae weight (mg) 6a 3P 1
Larvae weight (mg) -A-B 6
\\
p/
Weight per initial number oft
-C/ntilnumberlarvaC
\\
- v.
of
\\
V 21 1?'
Average weight per Initial number or larvae (mg) l,
- l. 1 Il Calculations and data revieed:
Comments:
lEnvironmental Testing Solutions, Inc.
I Page 3 of 6
.t I.:
i f<
I Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant Non-treated Date:
/
6 Q-03
-i P. -
Ir, I '
Survival and Growth Data Day 43.9%
72%
100%
M N
O P
QN R
S T
U V
W X
0
/tk
/
Jlo o
/0/0 6
/
A
/
/
/A I
~ ~
~
,' 6 / 0 e~~~~~~~
/ 0
/ 6
/ 6 0
/0,/
~
/
/
2
/()
t*
1r)
/D
/0/R/0I
/C /0
/0 o
10 16 o
lo 1o lo l0 to
/v
/o 16 10 lo
[a10 i
//
/
/0
/'
/0 f
/1 l
o0
/0)
/I
/6 1(
/(
to
/t
/0
/ 0 /0 i/
O
.~~~~
6~
l /0 A) 1a
/0 o
/0 lo{o1 / o
/o
/0 A-Pan w eight (mg g)
Larvne weight (mg) - A-B
-\\
__~~
Weight per Intial numnber of
$\\
O\\
'\\
dN
~ 0&
l
\\8
^$
~%J d
6 t
1arvae (mg)
ZD
-CIInitial number of larv~e
\\1 V-
\\
\\
\\
\\
\\
Average weight per initial numberof arvae(mg)
Calculations and data reviewed:
W Comments:
Environmental Testing Solutions. nc.
Pno. A r
EtSr.
4 A %J*
rf I -
Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant Non-treated Date:
/1M )O 3
Survivat and Grnwth nata Day 100% Intake Y
Z AA BB 0
/to
/6
/)
/0(
I
/16
/6
/0
/
. S c0 /0
/0 _C
_____4_
o 0
lo
/
10
/0 I9) 10 6
0 I) i
/0 A - Pan weight (mg)
B - Pan + Larvae weight (mg)
R Larvae weight (mg) - A - 8
, p Weight per initial number of larvae (mg) 6 C / Initlal number otlarvae V
\\
Average weight per initial number of larvae (mg)
Calculations and data revievwed:
Comments:
-~~~~~
Io Environmental Testing Solutions, Inc.
Chronic Vholc Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pineplalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Clitill:
Telt dates:
Project number:
TVA Sequoyah Nuclear Plant, Nontreatcd Octuber 7-14.2003 876 Reytiwtd b.
A U1'~
Ca.ceauif.Os R%
KepIKc 1
t,.IUas.f tt t t.tav.rt f
tetft A - P.n.t (mg) 11 -P P.
la".
I.anaeditbl(mg) Weigtil{oiUt.atrot M. sent*.
Mes lbdg(n)
C oat
(
tCtretin ts agbt (mg)
A. B t... (mg)
()
vadta
- 1) c tat l
)
A 10 10 14945 26140 11195 1.1195 Control D
10 10 14 881 2500 10159 159 100.0 1.0673 4.9 Not ppltabI C
10 tO 14 968 26020 11052 1.1052
- 1) 10 1
15 244 25 530 10286 1 0286 E
10 to 15079 26 310 11.231 1.1231 10.98%
to 10 15175 26 830 1165$
1.165 100.0 1.1321 3.3 4t1 C.
10 30 15 202 2604 101331 1 038 II 10 10 15240 26100 11 560 1.1560 I
to 10 15076 27.480 12404 1.2404 22%
J 10 10 15.106 26 160 It1054 1.1054 100.0 1.1303 4.9
.10.6 2
10 10 14 947 26 640 I1 693 1.1693 I.
10 t0 15 240 27.300 12 060 1.2060 A1 10 10 1510 27.510 12330 12330 43.9%
N 10 10 15 203 25 910 10.707 1 0707 100.0 1.1391 7.6 47 o
Ia-lo-JS221 251820 10.599 1 0599 10 30 15 213 27 140 11.927 1.1927 f Q
t0 10 15.199 26 850 11651 1.1651 72%
It 10 10 14921 24 440 912 0 9512 100.0 1.143 11.9
.7.6 S
1O 10 14 906 27.100 12 194 1.2194 300111 19..
T 0
10 14 934 27.510 12 576 1.2576 t1 10 10 14959 27.320 12 361 1.2361 V
I30 10 14902 26 100 11.191 1.1191 100%
10 I10 15 005 26 430 11.425 1.1425 100.0 1.1762 4.6
.10.2 X
10 10 14901 26970 12062 1.2062 0
10 10 15023 26440 11417 1.1417 100/.Inta~~~~~~e 30 3~~0 14 953 26 201.1711 100%/6 Intake 9W 30 9
15 020 26020 110o0 1-1000 97.5 1.1469 4.1 7.5 X
30 It) 15020) 27.140 12.120 1 2120 outrall let:
Iusinett's hMSI) value:
P!AlSI:
litabe:
Dunnett's ISD value:
PNIS):
MSD=
l'ISI) 0.1332 12.5 0.0616 6.4 kMininm Significant Difference l'acet Minnuim Significant I)iffrcnce PAMSI isa rnsue of test precision The PMSDis the nininunpacast diffatsce bctwttthtrto atnd am thdt cabe decwl statistically ignificAnt in a iKole cillucnt toxicity test On avaae a sigificant diflaa oecs foe Envirwmir Tetin Solutina. Ine.
chonic toxicity tests,,n atoxicant reduces Pimrephale growh by 17.0% fromthe control (detanined throh ttnfacuetdicant ti Lowse PMISD bound dtentnined by USEPA (I Oth pacentile) 9.4%
lt per PMSD bound determined by usEPA (90th percentile) - 35%
The lower and upper bounJs wcxe calculated by the USEPA using 205 tests conducted fran 19 laboatoris for Pinhale powti in chmnie rccrence toxicant tcsts USEPA 2000 Undestanding an Accounting for Mhod Variability in %hlIc Elaluent Toxicity Applications Under the National 'ollutant Discharge Eliffinstion Progam EPA-133-R,00-003. US Eznianta Protection Agency, Cincinnati, (311
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish crwi and Stvl TeaT-7 Day Crwih SaDt Doi 1017103 Tat ID-PpFRCX Sampl La Sequoyab Nuetr Plag. Intake.Nonteated Ed Due.
2W14 Lab ID ME mrEa.Tatbrg Soluton SAMPle Typc DMR-Discharge Ma eM4 Report Sample DaW Protocol CHRONIC.(EPA421-.42O23 Tet Species:
PPPiziephalea pevmelas Com%
I 1
3 4
DContr 1.1193 1.0139 1.1052 1.02t6 100 1.1417 1.1337 11000 1.2120 Transfom: Uatrssforied 1-Taild Istonc Cme.%
Mean N-Me Man Mi Mx CV%
N tat Critical MSD Mann N-Man D-Coarol 1.0673 1.0000 1.0673 1.0159 1.1193 4.929 4
1.1071 1.0000 100 1.1469 1.0743 1.1469 1.1000 1.2120 4102 4
.-2.25S 1.943 0.06t6 1.1071 1.0000 Auxitry Tests Statistic Critical Skew Kurt Shapiro-WIdrs Test idicate norma dastibutton (p >0.01) 0 9s652354 0.149 0.336540408 1.77063761 F-Test indicates equal variances (p-0 6) 1.250344515 47.46t34564 Hpoisesl Test (-tait. 0.0o MSDu
.MSDp 158 I£S F.Prob di HomoscedtctTestdciatesnosigncsnt d
erences 0.068563797 0.064240417 0012656405 0002489965 0065033890 1.6 Treatment vs D-Control Linear lalerpolation (2O0 Resamples)
Point SD 95% CL(Erp)
Ski IC05
>100 IC10
>100 IC15
>100 1.0 IC20
>100 0.9 IC25
>100 0.8 lC40
>100 ICS0
>100 0.7 0.6 e0 5 rc 053
.Z a.0.3 0.2 0.1
- 02 I
-0 2 0
- 0 40 60 0
100 120 Dose %
Das.e-pons Plot 14 1.2 1-tat. 00C5 level of sgnificance 0.
06 0.4 02 0
sqn_100703diatar
Environmental Testing SolutionsInc.
7~
`7 Page I of t
I Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia I
I i
I I
I I
I I
I Client: TVA Facility: Senuovah Nuclear Plant - Non-treated NPDES #: TN 0026450 Project :
ib Dilution preparation informaton:
Comments:
Dilution prep (Y) 10 8 Effluent volume (mL) 9 R-IW
)
/l)_9_1.5______
Diluent volume (mL) w f
/2*<
Total volume (mL)
Test organism information:
Test information:
Organism age:
42q-1)vXtS
_W) Randomizing template:
POepcg Date and times organisms
/67-63 075;Z i Incubator number and Z Dl were born between:
/Olls shelf location:
Organism source:
AA46-Qq-3o.03 A -b YCT batch:
fts_
___1__
Transfer bowl information:
pH f)
Temperature =2g.LC Selenastrum batch:f5
-. D3 Daily renewal information:
Day Date Test initiation.
Control water Sample numbers Analyst renewal, or batch used used termination time used used 0
l-2
/OI -01 l)j@-b3 0,8 30617-az /03 2
/O t~~t~()g l23I
/-010-0 03O>7. C2/. 43 2
R 0 5-3 1
3"I 12
-Ao8asr 0.5I)Oc oz.
J
_____ l-3 LzZR jlaso 5JC3 oI3io.Wc.U 6
70-1303 1Z1 o
/iDIOa 05101.s/
A 7
)0$.163 1 IY Control information:
JI Akccp afcC cnitena Summary of tt endpoints:
% of Male Adults:
C 1
61 t
s20% 7-day LC50
) I1%
% Adults having 3rd Broods:
D I
? SO%
NOEC 1
% Mortality:
47 I l s 20%
LOEC
> Ico Mean Offspring/Female:
A7.1
- 3.
15.0 offsprinrelmale ChV
%CV:
6 -
lb o
(40.0 IC25 I
I
i.
j..
I, II.
I I
I I.
I I
I I
I I
+-..
0 Environniental Testing Solutions, Inc.
Page 2 of 7 Species: Ceriodaphnia dubia Client: Se ular Plant Non-treated Date:
/1.
90 3 CONTRCT Survival and Reproduction Data Rplicate number Da 1 1 2
3 4
5 6
7 8
9 10 Youngproduced fQ a
0 0
0 0
J0 0
Adt mortalLty L
G l L L
Young produced 0
C Adult mortality A*,
L l
t_
3 Young produced V U
)
(
o Q
0 Adult mortality L
LU L.
1_
4_
1_
L 4
Young produced LA_6
-4 L4 A
L A Adult mortality L
i l
L L I
l L
5 Young produced JLj l
J It t
l IC)
O I
I O
\\
Adult mortality U
L U
6 Young produced lT C
[
O)
Adult mortality I.-,
L' l
U L Young produced 1
)(0 I
o j
ij jj..l.
1 1 j
& I r i_
Totalyoungproduced l5**
ll 33 S
3-5 l 3Z a 3 Final Adult Mortality 1
i i
i i
i X for 3' Broods l
>a l
Concentration:
l
% Mortality:
I OO I
Mean Offspring/Female:
32 COxC: 10.98%
Survival and Reproduction Data IRe licate number l
Day I_
1 2
1 3
_ 4
_ 5 S 6
1 7
1 8
I 9
1I Ip 1
'0 I
0z I 0 l
ll l
§ l
~Adult mortality l
_1 L
l lL Young produced Adultmort_
L-tyI I
3 Young produced
__IlIL IIAdult mortality L-
[
L_ IL_l 4
Young produced 1 Q T
_ s-(2 n
J s T I
l_
l~
-Adult mortality L-I L
l L
l L
l L
l__
s4 Youngproduced lSl Iz I
I 135 I
i o Adult mortality t,
l
l U.
~~
6 Young produced o
o
'l C
l l°l° Adult mortality T
__I U.
2 ~~~~~~~~~~~~~I 1
1L ToW youn: produced A
39 l 3 2 3
l Final.dult Mortality I
I
!L. L 1 2 lA I
I
- C I
Concentration:
I % Mortality:
O 07 Mean Offspring/Female:
B5.O
% Reduction from Control: 1 5'a I
L
1.lr 1'-&
Se.
I O I
.i Environmentn T ting' Solutions, Inc.
Page 3 of 7 I.
Species: Ceriodaphnia dubia Client: Seguoyah Nuclear Plant - Non-treated Date:
IO.o?*Q3 CONC: 22%
Survival and Reproduction Data ReUt nuwnber Da 2
3 4
5 6
7 9
10 1
Young produced QQ o
O n
o Aut mortallqy G
{
z 2
Young produeet C
)
O CO o
n Q c
Adultmoralty_
L-L L
3 Young produced t)
(^)
5 7)f A.dult mortalty L
L-
_l/,
4 Young produced
- 0....
S QS S
Adult mortality !
P S
Young produced 11 1 3 Adult mortality L
L L
L L
L 4
Youngproduced
_)
O O
C-)
o l
L Adult mortality lL L
L 7
Youngproduced zo iD iv I.
I, a
_L Ize I-i, Totalyoung produced T3 l =S L3 Final Adult ortality l
LL 4
jE LUl Concentration:
% Mortality:
007 l
Mean Offspring/Female:
3A4.°A
% Reduction from Control:
1.
CONc: 43.9%
Survival and Reproduction Data i____I _
Replicate number _
Dav l_I 2
1 3
1 4 1 5
1 6
1 7 l
8 l
9 1
0 I t Youngproduced j
0 J
° l
l
~Adult mortality l
I
(
I I(
Ir IL I
1 l
( lL o
2 l
Young produced l
T lli° l
l
~Adult mortallity l
_{
L l
l 3
j Young produced o (o j L
j ()
l 5 j ()
j t) j t
)
D 0
f )
O Adultmortality L-1 I
1 l
L t
l4 lYoung produced l5i(
I I
~Adult mortality
!L
!w!e S
i Youngproduced I
I It Adult mortalty Ll 6
Young produced l0 Adultmortality I U 7
Young produced I 2
I 2 I l LC J
l c
10 I -I I
l9 Total young produced lq.
q0 3q 3 q 3sl 3 L4 139 Ll 3
LFinal Adult Mortality l
c l
c l
l Iw AI Concentration:
% Mortality:
O I
lMean Offspring/Female:
l 3 S
% Reduction from Control:
1\\.Jo I
I Environmental Testing Solutions, Inc.
Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequoyah Nuclear Plant - Non-treated Date:
/O.O7-6 3 CONC: 72%
Survival and Reproduction Data
_._Reole number Day 1
2 3
4 5
6 7*
8 9
10 1
Young produced 0
O
()
D 0
Adult mortality L
L L
2 Young produced O
p p
p O
P 0
C Adult mortality t
t_
1 U
U U
3 Young produced O
Adult mortality L
L
'(
4 Young produced L
5
- S -
5 s
Adult mortality U.
.(
L S
Youngproduced IS 12-
%. I t IC) 13 1 1?3 I
l AdaItmortality t
L L-L-
L-..
I_
6 Young produced
)
C)
C Ci jO l
5O l Adult mortality
_ L L
U-1 i E 1 -
7 l
Youngproduced III zoT 0
t _
0 Totalyoungproduced t
35* l 31 I l 3 l3 l 3& l 35 3
FinalAdult Mortaitv L
=
L l V
Concentration:
%Mortality l Mean Offspring/Female:
ln l
% Reduction from Control: 1-IL*.7.
CONC: 100%
Survival and Reproduction Data I I 7
Repicate number l
Dv I
Y2 1
3 l
4 l
5 1
6 1
7 1
8 1
9 1
10 I
Young produced L)
° I 0 I 0>
l (
l I o Adult mor ity l
f j l L
(f Jl l
_j j
2 Young produccd 10O o o Q
Adult mortality l l ll 3
Young produced W if0 iLVI c
cLI Fn I
I vdult ort
,I T
I 4
Young produced Ls r
(0 l 5 l
5l 1 Adult mortality l
1 l
U l
I l
ll l
u 5
Youngproduced tic 1lZ_ l IZ
- 12.
- 12. -
l l I b-3 l !Y
_ _f AdultnmortalitY l
L j U 1_
U 7 C I-*
1 6
Youngproduced Cl l 7
l CTloll
)
°
)
_ _ I Adultmortality I l_
s]_ U -I_ L-E!
L.!.!
1 ! L !
I
.. 7 t Young produced l Z l
2.0 l.0 i's z c-Totalyoung produced t4Z.
3L1 l3 1 35 l3 8 l
-Lz 3l B3q9 3
qOq l FinalAdult MortVlity l
L.
l I l*
L l
I l 1 tL l
C l
Concentration:
%Mortality:
l 07T.
RMean OcspringFemale:
-S
- 7.
7
!% Reductionf&om Control:
i%
C.
-A-5,.:
I..
Environmental Testing Solutions. Inc.
Page 5 of 7 Species: Ceriodaphnia dubia Client: Senuovah Nuclear Plant - Non-treated Date:
/M. -6 9:
CONTROL Survival andReproduction Data Replcat number Day 1
2 3
4 5
6 7
a
=
lo I
Young produced O
Q
)
Adult mortallty LI L I U
U u
L I L
2 Young produced Q
C) 0
- C 0
L O
O A~dult mortaUity >.1 3
Young produced
)
c)
)
10 Adult mortality t-L 4
Young produced is Adult morallty t
I Young produced rL ti a
12-Adult mortality lI_
6 Young l
)
poc o
C1 0_
Adultmortallly I l t
-1 l
U..
l l
1 I
l 1
~.=--
=
7 Young produced l
I 5 l i
i l
llt I
l l
I zoII Totalyoungproduced at I 2 Z9 a-1 3-l 3 - l50
- 32.
3Yj L Final Adult Mortalty L
T '
I t
ll I
? 1 C Concentration:
lMean Ofsprngffcnale:
J?
3
% Reduction from Control:
CONC: 100% Intake Survival and Reproduction Data I
______RepLIcate number l
Dav l_I 1
2 1
3 l
4 l
5 l
6 1
7 1
8 1
9 1
10 lIlYouno produced l
(
I 0 l
I 0 O
l ()
l Iln)l°l(
I I
~Adult mortality C-(
-l I-Icl L, 1 L
L III 2
lYoung produced C
)l()
l 0
C:)
° 0
lOl(
l l
~Adult mortality L-l>ttl L
l>lW 3
Youngproduced o
t
)O Adult mortality l
2 I
l 1
1(l_
L__
__LILJ_
4 Youngproduced LA I
l
-l lAdultmortality L
L-l 5 yo ung produced j
I j lb j l j lz l
1 Flnal Adult mortality L
W
\\
t l
t 1
Ll 6
jYoung roduced l j)j O
)
i
° i
a i
o
[
i 9
1 1Adu 1t mortLllLtl L
tL 1
l 7
l
_____Youn produced l
Z_
l
_C_
I
_q r IP 1 11
-7 l,1 I
19 lFlnal~tdult lortality f2t_
Concentration:
%Mortalitv:
MOT..
I Mean Offspring/Female:
Sc 3bab
% Reduction from Control:
- 8 P,:.e<."
I'S' I..
1.I
Environmental Testing Solutions, Inc.
Verification of C'eriodaphnia Reproduction Totals Control-I Ile licate number Total Da'y tl 2
3 4
5 6
7 8
9 0
0 0
0 0
0 0
0 O
O O
O 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
6 4
4 4
5 4
5 5
47 S
14 10 10 I
10 13 10 10 0 13
)
6 0
0 0
0
-0 0
0 0
0 0
0 7
14 16 I8 16 19 Is 15 18 18 17 169 ITotal 34 30 34 31 33 35 30 32 33 35 327 10.98%
D
_Y R~eplicate number Total 1
2 3
4 5
6 7
a 9 I 10 I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
5 5
6 S
4 4
5 4
5 4
47 5
Is 12 10 15 I1 13 12 10 10 12 120 6
0 0
0 0
0
-0 0
0 0
0-0 7
19 21 17 17 38 19 18 38 21 15 183 T otal 39 38 33
- 37.
33 36 35 321 36 31 350 22%
lit Ilcate nuriiber thy l1 2 31 4 5
6 1
9 0
l Ioa l
o oI o I o 0 o 0 o 0
0 0
0 2
0 0
0 0
0 0
0 0-0 0
0 3
- 0.
0 0
0 0
0 0
0 0
0 0
4 5
6 5
5 4
5 5
5 5
4 49 5
14 30 13 12 10 2
12 12 13 10 118 6
0
- 0.
0 0
0 0
0 0
0 0
0 7
20
- 16.
17 17 21 20 17 19 17 IS 182 Total 39 l 32 l 35 t 34 35 37 34 36 35 32 349 43.9%
I Ile licate number I
1 2 1 3 1 4 6
7 S
9 1 10 I
0 0
0 0
0 0
0 0
0 1 0 1
0 2 3 0
0 0
0 0
0 0
0 II oo 3
0 0
0 0
0 0
0 0
0 4
5 6
4 5
5 5 5
6 4
5 50 5
16 13 10 12 14 10 12 16 14 11 128 6
0 0
0 0
0 0
0 U
0 0
0 7
21 21 20 37 38 20 17 17 17 19 187 Total 42 40 34 341 37 135 341.39 r35 3
6 72%
l y
I Replicate number Toa fla3y 4
5 6
7
-j9 I
eta r
r0 0 0 O I 0 0
0 i
0 0
0 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
5 5
5 5
4 5
5 5
5 4i 5
15 12 12 16 30 33 13 14 10 151 130 6
0 0
0 0
0 0
0 0
0 0
0 7
21 20 20 17 12 21 20 2220 18 197 T1'otal 40 37 37 38 8 33 33 i 3S 3
411 35 38I 375 100%
I i3ckte number 4a1 1)y 1 2
3 4
5 6 1 7 1
10 3
E l
0 0
0 0
0 0
O 0
O 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0.-
4 4
6 5
5 6
5 5
5 6
6 53 S
3 16 12 10 12 32 16 15 16 13 14 136
-6 1 0 0
0 0
0 0
0 0
0 0
0 7 22 36 20 33 201 213 18 18 15 20 138 otal 142 34 35 35 3s 42 3s 39 34 40 377 Control-2 l
I) l
~~~~Re plie number Tot Y
t 2 l 3 l 4 l 5 6 l 7l11l9l0 TeJl I
I O I 0 O O
o O
O o
I 3
0 0
0 0
0 0
0 0
0 0
0 4
6 r4 4
5
-6 5
5 4
6 1 5 50 S
12 10 10 14 14 14 10 l O11 t 12 117 6
0 0
0 0
0 0
0 0
0 0
0 7
20 16 15 IS 19 19 t15 18AS 17 14 171
!Total l3Etl3 l2 37l 39 l 3S 1_30 l32 l34 l31 338 100% Intake I
0 o o o o
o I o 0 o 0 o
o O
o 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0 ol o I o l
4 5
5 5
4 5
6 5
5 5
5 50 S
15 16 14 12 14 13 11 10X 13
.12
.130 6
-0 0
7 20 19 19 16 1 IS { 17 20 17 21 1
IS lTtll40l4l3Si3l37l6l 36 l 32 39 l3 6
Environmental Testing Solutions, Inc.
Chronic Whole Efluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyalm Nuclear Plant, Nontreated Client:
Test dates:
October 7-14, 2003 Project number: 876 Revelwed by:_
Concentration Replicate number Survival Average reproduction CoelIdnt t' Pcen redution tro;
(%)
1 2
3 4
5 6
7 8
9 10
(%)
(offspringIfemale)
(%)
Poold ntro
%)
Control-1 34 30 34 31 33 35 30 32 33 35 100 32.7 5.8 Not applicable 10.98%
39 38 33 37 33 36 35 32 36 31 100 35.0 7.6
-7.0 22%
39 32 35 34 35 37 34 36 35 32 100 34.9 6.1 67 43.9%
42 40 34 34 37 35 34 39 35 3S 100 36.5 7.9
-11.6 72%
40 37 37 38 33 38 38 41 35 38 100 37.5 6.1
.14.7 100%
42 34 35 35 38 42 38 39 34 40 100 37.7 8.2
-15.3 Control - 2 38 30 29 37 39 38 30 32 34 31 100 33.8 1.5 Notpp lcable 100% Intake 40 40 38 32 37 36 36 32 39 36 100 36.6 7.9
.8.3 Outrall 101:
Dunnett's NISD value:
PhSI):
Intake:
l)uu1ett's MSI) value:
PNISD:
2.581 7.9 MSI)
Minimum Significant Difference PMSI) -
Percent Minimum Significant Difference I'MSI) is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that ean be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests wvhen a toxicant reduces Ceriodaphnia reproduction by 9.9% from the control.
L.ower 'MSI) bound dcteranined by )SElPA (10 percentile)
I I%.
tipper PMSD bound determined by US EPA (90"' percentile) - 37%.
The lower and upper bounds were calculated by the USEl'A using 393 tests conducted from 33 laboratories for Cerlodaphniareproduction in chronic refcrence; toxicant tests.
2.649 7.8 USIPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications tinder the National Pollutant Discharge Elimination Program. EPA-833-RO00-003. US Environmental Protection Agency, Cincinnati, Oll.
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphels Saerh'l sad Reproduedto, Test-Repruddtlo.
StafDetar. l0'703 Tat.
CdFRCR Sample esisq yalt Nisc..' Plant. Ot 1 I 01 End Dtoe 10/14/03 Lab >.
ETS-Ev. Testing Solutons Sample Type DMR DischaW Monai% Report Sample Dos Protocol:
CWRON
.EPA421.R.02.013)
TotSpocus CV1Cerodapuinidbit Cacm.%
1 2
3 4
5 6
7 3
9 la DCmrtrol 34.000 30.000 34.000 31.000 33.000 35.000 30.000 32.000 33.000 35.000 10.91 39.000 38.000 33.000 37.000 33.000 36.000 33.000 32.000 36.000 31.000 22 39.000 32.000 33.000 34.000 35.000
. 37.000 34.000 36.000 35.000 32.000 43.9 42.00 40.000 34.000 34.000 37.000 35.000 4000 39.000 35.000 35.000 72 40.000 37.000 37.000 31 000 33.000 31.000 31.000 41.000 33.000 31.000 103 42.000 3400O 3s000 35.000 3.000 4200D 31.000 39.000 34.000 40.000 Trasdorm Untrssrorsud I-Talled leotowic Coae.%
Mos N-Mss Moss Mt.
Max CV%
N t-Stst Criticsl MSD Moss N-Me--
D-Coenntrl 32.700 1.0000 32.700 30.000 35.00o 5.775 10 33.717 1.o0 10.9 35.000 1.0703 3s.000 31.000 39.00 7.619 10
-2.037 2.287 2581 35.717 1.0000 2
34.900 1.0673 34.900 32.000 39.000 6.101 1o
-1.949 2.287 2.51 35.717 1.0000 43.9 36.500 1.1162 36.500 34.000 42.000 7.883 10
.3.366 2.237 2.381 35.717 1.0o 72 37.500 1.1468 37.500 33.000 41 000 6.061 10
- 4.252 2287
. 2.581 35.717 1.0000 100 37.700 1.1529 37.700 34.000 42.000 1.204 10
-4.429 2.2S7 2.511 33.717 1.0000 Ausilsey Tests Statistic Critical Skew Kurt Kalmogorov D Test usscates nrna diatrbutsion(p >0.01) 0.7s230977 1.035 0.19942737.0.7217265 Bartletes Test indicates equl varance (P - 0.70) 3.02303815 15.063171 llvpothesis Test (1-tail. 0.05)
NOEC LOEC CbV TU MSDu
%tSDp
.tS8 bSE F-Prob dr Dunetrs Test 300
>100 1
2.58144757 0.07894335 36.0166667 6.37222222 2.9E.04 5.54 Treatrenli vs D-Control Linear Inlterpolation (200 Rejamples)
Point V
>I00 IClO
>100 ICIS
>100 1.0 1C20
>100 0.9 1C25
'300 0.8 IC40
>100 0.7 Icso
> 00 0.6 0.5 0.4 0
V 0.3 V.
cc 0.2 01 00
.0.3 0
20 40 60 80 T00 120 os DoseResponse Plot 45 40 35 tail 0 05 tevet
.. 3
_f sgsfcance 025 t0 0.
S O-~~~~~~~~~~~~~~~~5 a
smn10-0703datax
Environmental Testing Solutions, Inc.
Statistical Analyses Colodispll S Svli and Reproduct Test-Rcaprodction Starti:
IOf7/03 Tet lD CdFCR Sunpl. W.
3qucysh Nucar Plat kIant End flte:
I WI 403 Lab D LTS-Enr. To" Solutios empb. Type:
DiMokorkng Rpot Seaple Data Pteotl P
CHRNICE1A21R02-013)
Tat Spec:
Q Ci i
- usib, Cmn C~eac%
I 3
3 4
S 7
3 10 D.Catrol 33.000 30.000 29.000 37.000 39.000 33.00 30.000 32.000 34.000 31.000 100 40.000 40.000 38.000 32.000 37.000 36.000 36.000 32.000 39.000 36.000 Transform: Untraaseormad I-Tailed lotoek Cec.%
Mea N.M*.a Mesa bi-Mal CV%
N
-Stsut Critical MSD Mesa N-Mesa D-Control 33.800 1.0000 33.800 29.000 39.000 11.484 10 35.200 1.0000 100 36.600 1.02S 36.600 32.000 40.000 7.856 10
-1.833 1.734 2.649 35.200 I.0000 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wfiiks Test indicates normal distribution p > 0.01) 0.92713833 0.6S
-0.0423974
-1.364012 F-Test dicates equal variances (p -0.38) 1.S225t07 6.5410S572 lypothesis Test ft-tAil. O.OS)
- MSDu MSDp MSSB MSE F.Prob dr HomosCedasuctTest indicate nos igicant differences 2.64S82503 0.073676 39.2 11.6666667 0,08339144 I. IS Treatrnents vs D-Control Linear Interpolation (200 Resamplts)
Point SD 9S% CL Skr IC05
>100 IC=O
>100 ICIs
>100 1.0 lC.O
>100 0.9 IC_5
>100 0.5 IC40
>100 IC50
>100 0.7 0.6 C
0.t s.0.3 0 2 0.1 0 0 42
-0.21 0
20 40 60 80 100 12.
Dosa %
Do.e-Response Plot 45 40 35:
- 30 0
10 5
....... I 1-tail.
0.05 level of significance 154 6
8 sjqs.j-07.OJJata.xlh
r.
I ~- -Environmental Testing Solutions, Inc.
I Page I of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Seauorah Nuclear Plant - UV-treated NPDES #: TN 0026450 Project #:
t, Dilution prearateon information:
Comments:
Dilutionprep(%)
ii)q6 Z7 2
Effluen vlumc (mL) 7.
JD?5 0
.W Diluentvolume(mL)
/qq1 I/42.5I I 0 Tots volume (mL)
-,?!D Ofto WSW!
&to!
Test organism information:
Test information:
Organism age:
2.S Z 71 P
OCh Randomizing template:
124b Date and times organisms i - o' -cs3 Iaz5o
,ro 1oo Incubator number:
13 were born between:
H-f
_r 3_1 Organism source:
- ~_
8i6AtCA lo-CS -iol Artemia lot number:
d qo, Transfer bowl information:
pH Temperature =
°C Total drving time:
II ADe
- 1. 8Z 2L.1 Date/Timeein:
t"1 Average transfer volume:
Date / Time out: lo5-Oventemperature:
O=
Dailyfeeding and renewal information:
I I
I Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding renewal, or batch used used time time termination time 0
.10 Ib u3
- 7.
lo-OL.-03 0: I
.oaz/
a3 loo-Ocm vs j
1.2A's, l0-O(0Cf'6 CIZ IO 7. a-Z/. 03 4 2
)}0- {3 O
ISIS lZlZ_
lo4 O----
ol
) o-l ISO 17SS3 as InO9 5
OZ-8a J3oi.as 0
\\.
D-IZ-03 OB50 (OOV (1`5 I O loaulo
.sl 1.X(
6
)bi0--6 09o02-VS r
10-6c azA5 JlltS CSO; -
O-I_
tz_
I I
Control information:
Accptance cntena Summary of test endpoints:
% Mortality:
l t07 S20%
7-day LCso
> I c7 Average weight per initial larvae:
j I. I 51L4 A
,3
-¢I NOEC l
I5)
Average weight per surviving larvae:
I.151 2 0.25 marvae LOEC
> I MI ChV I
ICs i >0O%
I
Environmental Testin2 Solutions. Inc.
Pag 2 of 6 Species: Pimephakespromelas Client: TVA I Sequoyah Nuclear Plant UV-treated Date:
/6.0 Z63 Survival and Growth Data Day CONTROL 10.98%
22%
A B
C D
E F.
G H
I J
K L
0
/ 0 1 0 / 0 /
/
0
/0
/
/
/
/
I 1
lo i01010/C)to10
/0/10 0
/0
/0
/
1
/D
/O la o1 0/
0/
/
/6
/0
/0 /0
.10 v
1/
O0 10
/0 jo
/0 /0
/
/0 /0 4__
tl
- k. Kc) to it) o
/I C) /0 to 1 6 /0
_S lb
/cD ID 11 10 10
/ l 0
10 0 (0 /0
/0 6
/6
/0 /0
/0
/0
/0 /0
/0
/o
/o I0 (0
_7
/0) ll /0/ 0 / 0 1 /(
/ 0 1
/
/0 /0) /0 IL) 7~ ~ ~
~
~
~
~
~
A - Pan weight (mg) v3 B - Pan + Larvae weight (mg)
_rI l
Lrvaeweight (mA-B
) - A - 8Y P
A
(\\ _
Weight per initial number of
~
-9
~
~
, ~ ~ -
5 i
larme (Mg)
\\ W i
0.
\\ r
-C Initia number oflarvc
\\
Aver2ge weight per initial numberotlarvac(mg) 1, 306
- l. 1bt Calculations and data reviewed:
Comments:
, Environmental Testing Solutions, Inc.
Species: Pimephalespromela Client: TVA / Sequoyah Nuclea
,i UV-treated I
III.
S r Plant Survival and Growth Data 7.,
£
'S Day 43.9%
72%
100 M
N O
P R
S T
U V
W I -
I 1.
/
O Z
1
/o
/6 lo 15) 1, sO //)
/
D ID/
lb /)
1 //0 16
/0
/O /'6 IC,4
- 2.
/O
/O io/L10
/LO
/O
/O
/0
/30 l 4
l01 i/o jO 1o o
1
(
/0
/
/0 s 6
}b /o
~~~/V
/O 10 Po
(
o
/t{
d
{
J~~~~~~~J9 QIOa t
/
/0 Ito IC) iv 1 /0/01 6
/0
/0 10 /0 10 /0
/0 /0
/()/t
{(6f/(
6
/0 7
()
i,Jo IC)
/0/0
/0 1O 10 /O
/O A-Panweight(mg) 77
_ l_
B - Pan 4 Larvae weight (mg) 1 t
,¢ 0
1 l
Larvae weight (mTZ) -AB A
'\\
'\\o
\\
9 Weight per iitial number 1
12rvae (mg) o
- C nitat number o larvae 5]
l
\\-
Average weight per initial numberof larvae (mg)
I, 1
Calculations and data reviewed: CA Comments:
Environmental Testing Solutions, Inc.
Vt
~~Page 4of 6
~~..
i.
Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant UV-treated Date: /O-t1743 Survival and Growth Data Day 100% Intake Y
Z AA BB 0I/D
/D
_0, 1
/a
/O
/0 2
/O l/o
/o
/0 4
)/ /t)
IC) l/
5 1~0
/0 /0)
/0 6
0
/O 10 7 ~~
1 q, A I t A - Pan weight (mg)
V 6:P 0v B-Pan Larvae weight (mg) e l
LarVaeweight(mg)-A-B Weight per Initial number o I
° larvae (mg)
~@
1.
-C Initial number of rvae
\\ *
\\
Average weight per initial number o larvae (mg) l, Calculations and data reviewed:
Comments:
Environmental Testing Solutions, LLC Chronic Vholc Effluent Toxicity Tcst (EPA-821-R-02-013, Method 1000.0)
Species: Pimephtalesprontelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant. UV Treated Client:
Test dates:
October 7.14, 2003 Revelwed by.
f
~
Project number:
876 Cascoosrotlo ('A)
Roplicmle Ilolli
- .mbr or rsl mombor t
lorvo A -lo RithI
(.)
B - P.. + L.r-e se. s
.I e* tag) Woighl Ilda*1 s-0breo M-oseniw M..s whigh (WC) Cfil r
lrbte,uhI s
d lar..
W.Ilght Ie)
-A.
B lar.tea)
(%)
((mg))
stl
( )
A 10 10 14.950 25.640 10.690 1.0690 Control B
10 0
14.905 26.460 11.555 1176 100.0 1.1574 5.3 Notapplplable C
30 I30 15.234 26.960_
11.726 1.1726 D
10 10 15:234 27.560 12.326 1.2326 1
10 10 15.214 26.140 10.926 1.0926 10.98%
F' 10 0
15.217 27.210 11.993 1.1993 100.0 1.130t 4.6 2.3 10 10 15.301 26.740 11.439 1.1439 i
10 30 15.245 26.120 10.875 1.075 I
210 to 14.970 26.760 11.790 1.1790 22%
J 00 10 15.285 26.330 11.04-5
.1045 100.0 1.1015 31.0 4.3 K
10 10 15.093 24.390 9.297 0.9297
__ 1.
t0 10 15.144 27.070 11.926 1.1926 l
10 10 15.023 26.50 11.557 1.1557 439%
N 10 10 15.125 27.20 12.155 1.2155 100.0 1.1689 4.1
.1.0 O
10 30 14.958 26.930 11.972 1.1972 P
30 10 14.968 26.040 11.072 2.1072 0
20 10 15.165 25.250 0.085 1.0035 72%
It IJ 10 14.9SS 26.360 13.372 1.1372 100.0 1.1379 11.2 1.7 S
~~10 30 4.93 2.00 13.17
.317 T
30 10 15.090 26 030 10.940 1,0940 U
10 30 15.202 27.610 12.40B 1.2408 100%
V 30 10 15.069 27.730 12.661 1.2661 100.0 1.2389 1.7
.7.0 Y
0 10 14.955 27.110 12.155 1.2155 X
30 10 15.240 27.570 12.330 1.2330 Y
30 10 14.992 27.240 12.248 1.2248 1000% Intake Z
10 30 35006 26.980 11.974 11974 97.5 1.1694 7.7
.1.0 AA 10 9
15.302 25.650 10.34 1.0347 3121 20 3 0 15.003 27.210 12.207 1.2207 Outrall 101:
Dunnett's NMSD) value:
PAISI):
Intake:
MulettIs NPII) v&alue:
I'NISIM:
0.1408 12.2 0.3098 9.5 NISI)
- Minimum Significant Difference l NSSI) -
Iercent Minimum Significant Difference lNlSI) is a neasure of tcst precision. is lMtSD ist he ninimum percent differenc between dhe contrlad tcabn thut em be declared statistically significant in a whole cmuent toxicity lest. On avcrage, a significant differnce occtirt for Eu oanent Tesing Solutions. Inc. chronic toxicity tests when a toxicant reduces Pimelhalea growt by 17.0% fiom tie control (detlemhed dough rcet stere tw,'kant testing)
Lowcr l'NMSD) bound determined by USEI'A (10th percentile) - 9.4%.
Uipper l ISD3 bound determined by USEPA (90th percentile) 35%.
Ilic lower and upper bounds were calculated by the USEPA using 205 tests conducted fror 19 laboratories for Pimepsales gowsth in chronic reference toxicant tests.
USEPA. 2000. Undertanding and Accounting for Mlethod Variability in Whole Elilhunt Ioxicity Applications Under the National Pollut nt Discharge Elinination Program. EPA-8333-R.OO-003. US Erivionmental Prtection Agency.
('iflcifulaii, 011.
Environmental Testing Solutions, Inc.
Statistical Analyses rl nidh Grwh %ad Suintvas Tert-7 Day Crwtk 3tar DWe:
IO 7/03 Test ID, PpFRCR 3
pW W.
3.qucahs Nuclu Pln.4 Oull 101 UV Tred End Dw:
30'3413 Lab ID.
ETS-Enw.
Teatg Ah~mu Spl Typo D
-bRaep Mordkt Repot snp Date P
ot CHAOHIC.PA421-R42013)
Tost Spedi PP-PrWOe pansle C
Ce.%
I8 2
3 4
DCmtol 1.0690 1.1S35 1.1726 1.2326 10.9 1.0926
.1993 1.1439 1.071 22 1A90 1.1040 0.9291 1.1926 43.9 1.117 1.2113 1.1972 3.1072 72
.0081 1.3372 l117 1.0940 100 1.2408 1.2661 1.2155 1.2330 Trnsform: Utrarormed l-Talled luetank Cosc.%
Moss N-Moss Mos Ml*
Max CV%
N t-Stat Critical MSD Mesa N-M&an D-Cotrol 1.3174 1.0000 3.1374 1.0690 1.2326 3.839 4
1.1574 1.0000 10.98 1.1308 0.9770 1.1308 1.0873 1.199 4.623 4
0.415 2.410 0.1408 1.1536 0.9984 22 1.0135 0.9516 1.1015 0.9297 1.1926 10.974 4
0.958 2.410 0.1408 1.1556 0.99S4 43.9 1.1689 3.0099 3.1689 1.1072 1.2153 4.119 4
.0.196 2.410 0.1408 1.1356 0.9984 72 1.1179 0.9S31 1.1379 1.0085 1.3117 11218 4
0335 2.410 0.1408 1.1556 0.9984 100 1.2389 1.0704 1.2389 1.215 1.2661 1.697 4
.1.394 2.410 0.1408 3.1156 0.9984 Auxiliary Tests Statilic Critical Skaw Kurt Shap-Wilk Tescates nomaduaftbu wo(p > 0.0) 0.97380805 0.S84
- 0.123S307 1.141763S7 Bardetrs Test ndicates equal wrnces(p- 0.101 93074211 150863171 HIypethese Test (1.l11. 8.95)
NOEC LOEC ChV TU M5Du
!ISDp MSs MSE F.Preb dl Durnete Test 100
>300 3
0.14080683 0.1216526 0.00S77711 0.00682721 0.3131732 61.8 Teatmrentss D-Control Unear Interpolatlon (200 Resamples)
Point S SD 95% CL(Exp)
Skew ICOS
>100 lC1o
>100 CIS
>100 1 0 lC.O
>100 0.9
[C.
>100 0.8 3C40
>3000.
IC50 100 06 0 6 0.4
- 0.
0.3 02 0.1 00 I
.0.1
.02 0
20 40 60 s0 100 320 Dose %
Dse-Respfnse Plot 1.4 1-tasl. 0.0S level
~~~~~~~~~~~.
I.c~
.njficance i0aao 6 04 0.2 0
iv as 4r 8
sqnO.07.03datx1S
Environmental Testing Solutions, Inc.
Statistical Analyses LArval ?isb Grwtk ad Survival T
?t-7 Day Growth Stan Dets:
It7J103 Tat LD:
PpFRCR SaMsID:
a) 3equoyah Nuclea Plsnk Wake UV Trat End De:
I(Yl4103 Lab [.
TSEnv. Teg Soluions Spl Type:
DMR.Dieharp Monriq RepoWt Spb Dat Probcol CHRONIC<EPA421-R02-03)
Tat Speces:
pPunepbepe poma commut C*ae.%
1 2
3 4
DCool 1.0690 1.1555 1.1726 1.2326 100 1.2248 1.1974 1.03U 1.2207 Tradrm: Untransermed I-Tallsel betlei CaR -%
Mesa N-Mesn Mesa Min Max CV%
N I-Stat Crittlal MSD Mesa N-Me.
DCoctoI 1.1574 1.0000 1.1574 t.0690 1.2326 5.339 4
1.1634 1.0000 100 1.1694 1.0104 1.1694 1.0348 1.2248 7.744 4
.0.212 1.943 0.109S 1.1634 1.0000 Auxiliary Teta Statstic Citleal Skew Kurt Shapiro-W&S Test amcates nonnl dstibunon(p>0.01) 0.S6909294 0.749
-1.1182024 0.12075012 F-test indicstes equal wnusces, (p - 0 64) 1.79521036 47.4683456 llypothesls Test (1-tall. 0.0s) btSDu MSDp MSE MSE F-Irsb dt Homoscedastc I Test mdsates no sutcan dilferences 0.10978863 0.09485596 0.000288 0.00638438 0.83883339 1.6 Treament s D-Conrol LWnear Inerpolaln (200 Reamples)
Peint SD 95% CUExp)
Skew ICOS o100 lClO
>100 IC15
>100 1.0 IC:0
>100 0.9 IC25
>100 IC40
>100 0.8 IC50
>100 0.7 0.6 C 0.5 0
0.4 or03 0.2 0 1 0 0
-0.1 0
20 40 60 80 100 120 Dose %
Dese.Rtsponso Plot 1 3 1.25 1.2 115 n1-tail, 0.05 level P
t 25 of signilicance 0.95 09 sqn10-07.03dzaxS
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pinephalesproinelas Daily Chemical Analyses Client:
Seqt Test dates:
Oct(
Project number:
876 zoyai Nuclear Plant Non-Treated
)ber (17 -14, 2003 Reviewed by:
(A-l
-l
- *. A Be I
I I
Concentration Parameter Dlay 0 I
Day DilI (Sul Il l Final [ Initial l Fnal Inital I nal
'.81 7.41 7.50 7.52 7.64 7.50 7.9 7.8 8.1 8.0 8.1 7.9 l
=
i l-9 l
w s
i DO0 (Ml/.
Conductivity (timhostcm)
Control Alkalinity (mg/(I. CaCQ) 291 60 86 24.6
- Intl l I 2l IHardness (mg/L CaCOQ)
Temperature C) pII (SU))
)O (me/I,)
10.98%
Conductivity (fiumhos/cm) 4-Tleniperal ire (Cr) 25.6 1 25.2 I(
25.1i 24.(
24.9 25.21 25.01 24.91 25.11 25.51 25.2 t
1 ~~~~~~~~~~~~~~-
I I I nil (SU) 7.51 7.33 7.551 7.52 7.5 7.46 7.651 7.061 7.661 7.041 7.631 7.5.
22%
)O (mg/l,)
8.2 7.7 S.2 7.8 8.1 7.9 8.3 6.5 7.6 6.21
-7.8 7.5_
Conductivity (Imtihos/cin)
I 275 268 265 A
1
,1 1
. 1
-- -1
-- -J
-1
-- -1
-- A
-- -1
--- i Temperature (-C) 25.6 25.2 25.6 25.1 24.6 24.9 25.2 25.0 24.9 25.1 25.5 25.2 25.3 24.7 pil (SU) 7.56 7.27 7.52 7.50 7.55 7.44 7.64 7.06 7.56 7.06 7.54 7.51 7.64 7.07 4.1.9%
')';z lu) (llag I
7.7 x.2 7.0 8.2
- 7. x.3 f6.7 7.6 6.2 7.9 7.5 7.8 6.2 4
(-'lConuctivity (jimliok) 248 241 235 240 243 239 237 Temperature (C) 25.6 25.2 25.6 25.1 24.6 24.9 25.2 25.0 24.9 25.1 25.5 25.2 25.3 247 pil (SU) 7.53 7.32 7.48 7.49 7.50 7.46 7.61 7.08 7.45 7.07 7.44 7.51 7.62 709 72%
i)O (mgIl,)
8.2 7.8 8.1 7.8 8.1 7.8 8.3 6.4 7.6 6.3 7.9 7.5 7.8 64 Conductivity (pnmhos/cm) 213 208 203 205 206 204 203 Temperature (°C) 25.6 25.2 25.6 25.1 24.6 24.9 25.2 25.0 24.9 25.1 25.5 2S.2 25.3 24.7 p I (Su) 7.51 7.40 7.41 7.48 7.46 7.46 7.58 7.15 7.33 7.07 7.37 7.59 7.05 1)O (mgilL) 8.1 7.7 8.0 7.8 8.0 7.9 8.3 7.0 7.6 6.3 7.9
- 77.
7.91 6.3 Conductivity (ilmhos/cm) 178 174 172 168 171
- 169 169 100%
AlkalInity (mg/L CaCO) 66 6
62 Hardness (mg/L CaCO 1) 71 69 69 Total Residual Chlorine (mg/L
<0.10
<0.10 Temperature C) 25.6 25.2 25.6 25.1 24.7 24.9 25.2 25.0 24.9 25.1 25.51 25.2 25.3 24.7 pil (SU) 7.52 7.38 7.42 7.51 7.45 7.47 7.56 7.09 7.24 7.05 7.28 7.52 7.58 7.09
)O (mg/lQ 8.1 7.7 8.1 7.8 8.1 7.9 8.3 6.9 7.5 6.2 8.0.
7.4 7.9 6.8 Conductivity (pmhos/cm) 179 170 169 169
.167 169 100% Intake Alkalinity (mg/L CaCQ) 64 62 62 Hardness (mg/L CaCOj) 69 7!
'69 Total Residual Chlorine (mg/l
<0.10 co.I0
<0.10 1_Temperature (C) 25.61 25.21 25.61 25.1 24.7 24.91 25.2 25.0 24.9 25.11 25.51 25.21 25.31 24.7
I I.
I Ir I
i Environmental Testine Solutions. Inc.
Pie 5 of 6
- -I- - __ -
Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Daily Chemtstry:
Date:
1O
.b3 rI.
Day I
0 A. -
O, I.w A
gV /M I
^nKfle A
-1 Analyst I Concentration Parameter CONTROL pH (S.U.)
DO (mg/L)
Conductivity (u1mhostcm)
-r~~~~
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO,/L)
Temperature ()
pH (S.U.)
10.98%
DO (mc/L)
Conductivity (omhos/cm)
=
r Temperature (CI
.1 1
J
pH (S.U.)
22%
DO (malL)
Conductivity (umhos/cm)
Temperature (C) pH (S.U.)
1%574 43.9%
DO (mg/L)
-24.5 4+s
_Imi 1
_I I
14
~
1 I
lb Conductivity f1,Mhn(/iMN zq6 HOMMM
__. __I ___ ______
~
a Temnerature (fC)
Z
.b ZS.2,
'S. &0 ZS 1 Zq.
-ZLI.q I
I pH(S.U.)
if 3
.3-l -?4 l
o;-q l.so 72%
DO (malL)
Conductivity
-13 Temperature(°C) 4 Z52 Z.(
25
?L7t, 21 pH (S.U.)
-,1_tD t,
100%
DO (mJlL)
. I 0-V T.
Conductivity (pmhos/cm) szT 1t<W5 Alkalinitv 1
\\
y (ma CaCO~/L) tot0 h=lZ Hardness (mg CaCO 3/L) 3 TR chlorine
\\
- 0. I (ma/L)
<)/
t
@0 Temperature (C)
Z5. to 2S.Z l
Zs 'L
" 7 2.9 pH (S.U.)
.152 I-3 :I.?-
%57 IdN 100%/ Intake.
DO (mJL) 1 ll 141 Conductivity (umhos/cm)
_______l(_
Alkalinitv (ma C3O/L)
(,o Hardness (m_ C3CO3/L) l 0g TR chlorine (ma/L)
Z./
O.
_________Tempera~re (0 )
ZS.Z..
Zs.(O
~
m'7 2
I I
I I
I rnitinl II Final I
[nitial I1 Final Initial 11 Final I.-
Environmental Testing Solutions. Inc.
Pa.io, A rr E-vironmental Testin 6olutions, Inc.
vop Species: Pinephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date:
16-63 I_I Dav I
a a
3
-I-r I
4 Analyst I
I Concen-Parameter tratlon I
CONTROL nH (S.U.)
J.
I it I
L.I I
DO (m2L)
M.3 I
Conductivity
(,umhostcm)
?281 Alkalinity (mg CaCO3IL)
Hardness (mg CaCO3/L)
I,\\,
-1.
E
IZL
-Ju 4
_q LF 2 lq K;.
Temperature (°C) 2S.2
- 7.
I E L4 ZS.W
,(4AB 4_-4 9'I
~
ii -
DIH (S.U.)
i1 10.98%.
DO (mglL1 Conductivity 2 It (unihos/cm)
_ _T ze'~~~ ____~1 zI Temperature (°C) 2..
I IZf l2d Q ZS,2 pH (S.U.)
I
_A_
22%
DO (melL) f?
Conductivity Z(o 8 Temperature (C) 25..
ZS:O dY.9 Z5 IT ZSZ Z-S. 3 2
pH (S.U.)
(0 2.OI
-4.54 _-51 S
W 43.9%
DO (m"eL)
-A (h9
. 5 71 e Conductivity23 3g23I (igmhos/cm) 2_
_e____2__
Temperature (C)
IZS *Z 79 j
2 ZS S.Z Z5' 3 pH (S.U.)
-,mq tZ-72%
DO (mg/L)
(0o73
1-.'i le, Conductivity --
1 z1E go oq EWA t(gmhos/cm)'
I os IgL1 Temperature (C) 2c.2 ZS.0 d
ZS-I ZS.2 Z.?
pH (S.U.)
-4 T S 2,3.
241
,3
.5I
- 59 9D 100%
DO (mglt)
- 1.
(.3 Conductivity (umhoslcm)
(oS 1
Alkalinity (mg CaCO 3/L)
Hardness
.(me CaCO3fL)
TR Chlorine (mg/L) 0.0 Temperature (C)
Z.
.1 Z5 I Z5, Zq.
T.
pH (S. U.)
Ct
- b_4.q
_L26 5.0 Z
l
.,SZ 55 e
100%
DO (mglL) e
- 3.
Intake Conductivity (umhos/cm) lot Alkalinity (mg CaCOi/L)
(pz Hardnessq (m? CaCO31L)
- 20.
Z5 Temperature (C)
.a j
z
- 2.
Z3 I
I I
Tni#inl 11 V.n.l I
T.it-sl 1i Finn I
Tnitial 11 I Fi 1 I
Tnitial I
final I
i
. *Xa1 r-
^s.z Ia&....., _.
Environmental Testing Solutions, Inc.
Chronic Vhole Efuent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia ditbia Daily Chemical Analyses Client:
Sequoyah Nuclear Pla Test dates:
October 07 - 14, 2003 Project number: 876 Int Non-Treated Reviewed by:
Cj I-Concentration Parameter
_~
Final Initial nil (I) 110 (mOI.1....
'"....I Cnntdcthvitv fumhoscm)
Control
,... _, I Alkalinity (mg/L CaCO1)
I-llardness (mgtL CaCOI)
Tem.erature 25.41 25.21 25.51 25.0
.91 i.,
.{
pi (SU) 7.58 7.57 7.57 7.63 7.61 7.61 7.66 7.70 7.74 7.79 7.67 7.64 7.68 7.6 10.98%M DO (mg/L) 8.2 8.0 8.1 7.9 8.1 80 8.3 7.6 7.6 7.8 7.9 7.7 7.7 7.
.8.
Conductivity (Itrmhos/cm) 286 276 261 270 28 i2 87 279 25 Temperature M) 25.4 25.2 25 25.1 24.6 24.9 24.7 24.9 24.7
- 25.
25.5 251 25.0 25.t pil (Su) 7.58 7.57 7.5 7.62 7.58 7.60 7.65 7.70 7.66 7.7 7.63 762 7.66 7.6 22%
)O (mg/L) 8.2 7.9 8.2 7.9 8.1 8.0 8.3 7.5 7.6
- 7.
7.8 7
7.7 7.i Conductivity (pumhos/cm) 275 263 259 263 268 265 26 Temperature (t) 25.4 252 25 25.1 246 249 24.7 24.9 24.7 252 2
251 25.0 25.1 1) i 0
I ni (I) 7.5 7.5 7.5 7.631 7.51 7.61 7.6 7.6 SI 7.5 7.7 7.5 7.6 7.6 43.9%
DO (m /l.)
_1 8.01 8.21 7.9 7917.8 7U 7t Conductivity (tmhos/cm) 248~t 241
~
233 240 243 239J.
23 Temperature ()
nil (Sl)
I-DO (me/.)
72%
5
- Conductivitv (imhos/cm)
I-Temnerature (C pit (SU) 7.511 I)O (mg/L) 8.1 Conductivity (limhosl/cm) 100%
AlkalInity (mg/L CaCO3) lHardness (mg/L CaCO3)
Total Residual Chlorine (mfL)
Temperature ()
1 25.4 7.59 7.50 7.58 7.61 7.66 7.45 7.67 7.44 7.60 7.9 8.1 8.0 8.3 7.6 7.6 7.9 7.9 7.8 203 20~5
~
206
~
204 25.1 24.6 24.9 24.7 24.9 24.7 25.2 25.5 25.1 7.58 7.45 7.52 7.58 7.62 7.33 7.61 7.37 7.60 7.9 8.C 7.8 8.3 7.6 7.6 7.8
- 7.
7.7 169 169 169 167 6'
7_
6 25.1 24.6 24.9 24.7 24.9 24.7 25.2 25.5 25.1 7.601 7.451 7.521 7.561 76 7.241 7.651 7.251 7.601 7.91 8.11 7.8 8.3 75 7.5 7.8 8/0 7
16 169 !-
71 69-24.6 24.91 24.7 49 24.71 25.21 25.51 25.1 nil (NI I
I DO (me/L.)
Conductivity (smhos/cm) 100% Intake Alkalinity (meIl, CaCO)-
hardness (mgIL CaCO,)
Total Rtesidual Chlorine (nl/l.) J
<0.
Temnerature (12)
Spilled sample.
r, I :
I i
I I
I I
I I
I I
I I
I I
Environmental Testing Solutions, Inc.
Page 6 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Daily Chemistry:
Date: - In-Dq-3 I
Day 1
Analvst I Concentration Parameter CONTROL pH (S.U.)
DO (m/L)
Conductivity (pnhos/cm)
Alkalinity (mg CaCO 1/L) 4 Hardness (mg CaCO/UU
_ _ _ _ ~~~~~~. 1 Temperature (0C) pH (S.U.)
-4.0 76 10.98%
DO (mg/L)
Conductivity (pnihos/cm) z et4 Temperature (°C)
Z7.
Z pH (S. U.)
5 22%
DO (mg)L)
Conductivity Z
l
_p e au e
(
0 )
2S Teniperature (°C) 5 2
nH S.U.
r E
43.9%
DO (mnM 7
I.
Temperature (C)
ZS.Z 2q.
wq.q pH (S.U.)
3 w5S
>§ 5
72%
DO (mgJL)
Conductivity Z13 2-3 Temperature (IC) zS.Y ZS.2.
ZS.S Z 2S. l Z'I. (
pH (S.U.)
I to 5
100%
DO (m/L) 46 Conductivitv (6xmhos/cm) r e Alkialinity (mg CaCOvL) to (
Hardncss (me CaCO/L) to I
~
\\
}
(m elL) 4l
- aL(
Temperature (C)
ZS.
ZS.
ZS1.(
l 2 qcq pH (S.U.)
4H7
~t.S*Z o
- q-1 1t 75 100% Intakc DO (m/L)
£
. II_
Conductivity 1 4_
(umhoslcm)
Alkalinity (me CaCO 3I)
(LP Hardness (ma, CaCOVU L)
TRl chlorine Temperature('C) z j
4 Z
ZS.
2cI.(.
Initial 11 Final I
Initial II Final I
Initial -II FinaL r
u--Z' C! wo-d Z
0;z- d f t&~
,, " "q '. ' ko"
P Environmcntal Testing Solutions, Inc.
Page 7 of 7 x.O.
[it Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date:
1.0-67-6:1 I.I.
Day I
i Analyst I C c Parameter trvtlo CONTROL I (S.U.)
I DO (mg/L)
Conductivity (timhostcm)
Alkalinity (mR CaCOtL)
^,
x Hardne"s (mg CaCO3/L)
Temperature (°C) 10 10.98%
pH (S.U)
=_ ~I x
~
D DO (m2/L1 Conductivity (gmho~m Temperature (°C) 3 16
-o to 2B:a 2 I.I
.?q-+
2qq 04A~w 1
47-(a5 I~
7.,L>o Th,
_p I_
1.-
I zs.S 7Ss.1 zs-O nH (S.U)
I_
III il -
r--x_-_-
22%
DO (ma/L)
\\---r Conductivity (umhoslcm)
I 103 2(pe
.2.A 11Z0 ZS~o zS-Dc Temperature (°C) 4 4 __________
~~~.
I
-irri.
pH (S.U.)
I 11 1 -4.5g t
I
43.9%
DO (mglL) 6.6 Conductivity 1
(umhos/cm)
I 2 0
243 Zaq Temperature (C) 2 L/.
- 4.)
4 4
I pH (S.U.)
-4.en to 7.q DO (me/L)
Conductivitv 1
1 I
(pmhos/cm) 5 2I E
Zo4 Temperature (°C) 2q. -t D 'I.0 23.2
)
-.0 1O 1 MS-. ___.O
-,cpz i ~2~
.1~(
25AD S2S
-+f3
\\Imu 7.
S J (U I
4 4
A.
4 pH (S.U.) IA,
-7.r n
- w.
- 7 toI 100%
DO (eL)
&B
- 4.
0
- v1 Conductivity (urmhos/cmn) 08 1+1(R Alkalinity (me CaCO31L)
I i (02 Hardness (me CaCO3L)
TR Chlorine (mn/L) 0 I Tcmperature (C) q.2 -
V I z
Z1 Z S.c)_E'.
pH (S.U.)
4 Z
J
.:7 CoO
(:;.5 I _____
100%
DO(mg/L) 7;6 7S 7;.
7 Intake Conductivity (umhos/cm)
Alkalinity (mg CaCO3/L)
(0Z Hardness (mg CaiCOV/L)
(
£\\0 Temperature (C)
I.s. Z Initial t
Final I
Initial Final I
Initial I) Final I
Initial I
Final
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pinephales pronelai Daily Chemical Analyses Sequoyah Nuclear Plant UV-Treated Client:
Test dates:
October 07 - 14, 2003 Project number:
876 Reviewed by:
Cap-Ye Concentration Parameter nH (SU) 4-DO (mFJI Conductivitv (umhostcm)
Control l
Alkalinity (mg/L CaCOj)
Hardness (mgfL CaCOW)
Temperature SC) nil (Sul DO (inc/L1 10.98%
Conductivity (umhos/cm)
.1
-e -l
-t
.l
-n Al
^^a Ad I NJ ^1 Temperature (C)
Z3.)I
/
.1L 25.71
.1 zq.
24.71 2)2 Z.0 Z5.1 Z4.91 Z3.I/
Z4L'J L).41 Z4-E p11 (SU) 7.56 7.39 7.60 7.57 7.60 7.45 7.63 7.17 7.51 7.16 7.59 7.54 7.67 7.10 2° DO (mg/L) 8.2 7.8 8.1 8.0 8.1 8.1 8.4 6.9 8.0 6.6 7.8 7.4 7.9 6.3 22
.Conductivity (pmhos/cm) 276 264 262 265 270 265 263 Temperature C) 25.6 25.1 25.7 25.1 24.6 24.7 25.2 25.0 25.1 24.9 25.7 24.9 25.4 24.8 p1 (SU) 7.55 7.38 7.58 7.54 7.58 7.44 7.62 7.15 7.47 7.10 7.56 7.54 7.67 7.09 43.9 DO (mg/L) 8.2 7.7 8.2 8.0 8.1 8.1 84 6.'
7.8 6.4 7.8 7.5 7.9 6.3 43.9%
X Conductivity (pmhos/cm) 249 239 237 241 245
_232
_237 Temperature (C) 25.6 25.1 25.7 25.1 24.6 24.7 25.2 25.1 25.7 24.9 25.4 24.8 e1 (SU) 7.54 7.43 7.5 7.55 7.57 7.45 7.60 7.16 7.41 7.06 7.48 7.53 7.64 7.04 DO (mg/L) 8.1 7.8 81 79 8.1 8.2 8.3 6.9 7.9 6.1 7.8 7.4 7.9 5.9 72%
Conductivity (zmhos/cm) 215 207 207 207 209 206 203 Temperature (IC) 25.6 25.1 25.1 25.1 24.6 24.7 25.2 25.0 25.1 24.9 25.7 24.9 25.4 24.8 p1 (SU) 7.54 7.40 7.51 7.53 7.54 747 7.59 7.12 7.35 7.07 7.42 7.49 7.63
. 7.00 DO (mg/L) 8.3 7.9 8.1 79 8.0 8.2 8.3 6.8 7.9 6.1 7.8 7.3 7.9 5.9 Conductivity (mhos/cm) 17 173 172 173 172 169 168 100%
Alkalinity (mgtL CaCOs)
Hardness (mg/L CaCO,)
Total Residual Chlorine (m/L)
Temperature (C) 25.6 25.1 25.7 25.1 24.6 24.7 25.2 25.01 25.1 249 25.71 24.9 25.4 24.8 p11 (SU) 7.51 7.42 7.47 7.52 7.52 7.44 7.52 7.151 7.31 7.04 7.381 7.52 7.621 7.021 DO (mpL) 8.2 7.9 8.1 7.9 8.1 8.0 8.4 6.7 7.9 7.8 7.4 8.0 6.5 Conductivity (mhos/cm) 17 171 170 171 169 167 169 100% Intake Alkalinity (mglL CaCO3)
Hardness (mglL CaCO,)
Total Residual Chlorine (mg/L)
Temperature l
25.6 25.11 25.71 25.11 24.61 24.71 25.21 25.0l 25.11 24.91 25.71 24.91 25.41 24.8
s Enviomna TAen Solutiosc Page S of 6 a
g S s _
Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UW-treated Daily Chemisry:
Date:
I0-043 I
Day I
An ys Concentration Parameter CONTROL pH (S.U.)
Conductivity (urhos/cm)
\\ =_
,~~~~~~~.
Alkalinity (mg CaCOvlL)
Hardness (mg CaCO3IL)
Temperature 9* -
P pH (S.U.)
r._~I
_~~--4D 10.98%
DO (mg/L)
_~4 _ _s Conductivity (pmhos/cm)
Temperature n
I TT 22%
P.
IIft v
y I
- 7.
j I
-I DO (m/L)
G.Z q S.6
- (
l Ii B.(l 6e Conductivity Z -io I
1 Z&
t Ternnerature (C)
-2 I 7ZS.S
-L5s. I L. 6 4 --
4 -.----
****
nH (S.U.)
- . 6b 1 5 b 4 -..-
=-=-
.9 --------.,
-.------
11 43.9%
DO (mc/L) 8.Z
- f. 2.
Conductivity Iq9
-M Z37 (umhos/cm) j
-1 23
. Ht 11 S 23,46 1 Temperature (°C) 25.1 7.S.3 pH (S. U)
-;.5
. ¶3 6: V5 72%
DO (mg/L)
]!.
=
8tl I Conductivity Z I_-
(umhos/cm)
S KW Temperature (C)
Z5- (o l*5.
- 25.
I nH (S.U.)
II I
ZI.
If I
4-=-I 9.-
100%
DO fmpIT-I1 I
Conductivity (Vmhos/cm)
I_____5 I
Alkalinity (me CaCO3/L)
\\
Hardness (mn CaCO-VL)
TR chlorine Temperature CT) 7z.S. o II 11 I
- 4.
I.
-
I pH (S.U.)
I I
100% Intake bo (mc/L)
Conductivity (jjrnhos/cm)
( I i
Alkalinity (mg CaCO3 1L Hardness (mg CaCO 3/L)
. I D Temnerature (C) 7'; (cd I---.
Initial
I I.
1..I I
I I
Environmental Testing Solutions, nc.
Page 6 of 6 Environmental Testing Solutions, Inc.
Page 6 of 6 Species: Pimephalespromelas Client: TVA Sequoyab Nuclear Plant - 1W-treated Date:
/.
Mb3 a
Day Analyst Concen-Parameter
',. il tration I
3 4
I
. 5
- 11
...:6 CONTROL pH (S.U.)
_~I-DO (mm/L)
Conductivity (umhos/cm)
_, _~I.
Alkalinity (mg CaCO3/L)
Hardness (mg CaCOJlL)
\\~~ ~
Temperature (C) 6~~~-r-------
^ -
I R
pH (S.U.)
10.98%
DO (mz/Ll Conductivity z3 (jimhos/cm)
I72.
Z I-1~
I Temnerature (°C)
ZS.Z.
Z5 2Y.94
. ~--
r-t 1 dl 6i _ ____11 I..
,b
.6 _ ^ 5 pH (S.U.)
-454.
.t II*-
t ------
U*
II
-----ll-I 22%
DO (mulL)
I 4.1 Di Conductivity (plmhos/cm)
I2/s NOW 0a is 005 Temperature (C) 2S.2.
2S.1 t.A4q IN 0 ZS a i
I lb l
pH (S.U.)
-4.(*
r.,
-- 1 1 -4.41 43.9%
DO (/L)
.4 Conductivity (tmhos/cm)
I t ---------
w -
r
~Z45
_1 Z
i2 23 Temperature (°C)
- 25. I Da 9 II
.1 6
- O I.
pH (S.U.)
O II1 1 4J I
r
~~I
-r
-+-
r-72%
DO (mlL) c,1
-+. It Ii Conductivity (umhoscm)
IZ.
j 41 4
II.in, Zoq.
Z 0O ZO3
.;I Temperaturc (C)
ZS. Ef q
L
.1 1
4 q.
- r pH (S.U.)
IL 100%
I DO (melL) 8 Conductivity (umhos/cm) 1 43 Alkalinity (mu CaCO3 L)
Hardness (mg CaCO/L)
\\
.q 1Z.I 41 I
--------
¶
- 7. 5 If--q
/ (PC at ~~~.\\1 II I
Tcmnerature (C)
I1 R24c I
it II
-- ~
~
r---.
.1
~~~~~~~f,~~~~~
I nf i
--j97 II 1= PC
- jAI II I 1,aZ 1I :1 U-.- ~ ~ )
I a
- l.
p I
- a.
r 100%
Intake DO (mg/L)
S. 4 -1 6 +/--
Conductivity (gimhosfcm)
H'1i Alkalinity (mg CaCOlL)
Hardness (mg CaCO/L)
-I 1 II I -
I'
-1(0-I S-eD A
\\
IN AL
- 9 w
._A/
b 11 _
B I
I A
I Iemneraturel I
I
.1.
11 ai te-,j It o 'Lr. 7
[ u 9.
'I It A, 'I,
I
.M W
r a
-I.
i I
M I
II 1 I
0n M:..
1 i I -T.#.
lI Ftina I I
[nilial II fin al I
SAL3141 1
I 5lt3131 Ij a I IlIhI Sll
_JI j
-a
Environmental Testing Solutions. LLC Page. -Jof Environmental TestinE Solutions. LLC Pane of Total Hardness (EPA Method 130.
Matrix Water, MDL - 1.0 ms
.Analyst Duplicate sample preciszon:
Sample Hardness
%RPD =
Sample Sample 1D volume Begin End Total Multiplier (mg CaCO1tL)
. (S -D) l(S+D)Y21l) 100 number (ml) ml ml ml (acceptb rp 10%)
\\ b b
- t:3 Yi\\S x(>
=
\\ \\ q
- .l
~~~~~~S 4z Duplice.
lD
\\
D 3.
Matrix spike recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO,/L)
(me CaCO/L)
(ml) ml ml ml Sample hardness (B)
Measured spike value (,M I%
R = MV / SV X 100 (mg CaCO>/L)
MV = A - B (acceptable range (mi CCOIL.
_ 75 to 25%)
Sample measrements:
Sample volume Begin End Total.
Hardness Sample number Sample ID (ml) ml ml ml
'Multiplier (me CaCOIL)
Blank CC.U 5 )C Z
~
9>
gb
~(shoud be0 mg CaC0,1L) !t tOlonto XCK w_
VA Rs
- 2. O 2
1
,7j es, 12.
b Q B l 1
13z.6 ~~~~~0 413W 8
E t 4 I !
.S 4.
,OO
. q h KiPUAiI 1l2__z__2 1
___1
?tloo.
e I) 4 Ii-.0I O.?
I I f L+/-L-1 Date reviewed [I2-I2Ii1iZZ Note: If >15ml of itrnt is used. sample must be diluted.
Reviewed by: I -A 71 C-, I
Environmental Testing Solutions, LLC Page Total Hardness (EPA Method 130.2) s Matix. Water, MDL - 1.0 mg CaCO3/L Analyst 1
W.
- 4 ^; ~ w P-X -b z**.@
-sf*¢
- 3adQ~~~~~(
S c
-itrant Nor 7...t
.c Total Normality(A) oEDTA pHFactoror i#
re.er ernce ni; =e z -
2£5K, m
sumber
&Cit"*~~NXO0S0nampe ccapb t
t osg~o.cR.
n~znier
-......... -. (EN)
- tste~bE~wt~xU__
0.022)
V "-IrtrzP Laborary canfrol.ntandard:
~~~~-
Reference True value Sample Kardness(MV)
%RS-MVI1TvxlO standard number c) volume Begin End Total Multiplier (mg CaCOIL)
(aeceptable range (mg CaCOstL)
(MD ml ml ml 90 to 110%)
2155i lbl) 40 SO5 50N q
t5/
Duplicate sam It precison:
Sample Hardness
- s)RPD Sample Sample II) volume Begin End Total Multiplier (mgc aCoep)
I(S -D) nl(S+Dy)
I 0) number
.(ml) ml ml ml (acceptable range -t:IG%)
plwoO.
AFLi A,
5- -
O Q9
- o.
S S
4
, k s t 1
0 Matrix spike recovey Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOsL)
(me CaCOL) ml ml ml
- ]iL~o O
S lql; l-S Sample hardness (B)
Measured spike value (,M V
% R = NIV / SV s 100 (mg CaCO5IL)
MV = A - B (acceptable range l
l (mg Cacs/L) l
- 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness Sample number SampleID
-(ml ml ml ml Multiplier m CaCOVLU Blank
_.IIl-l ___________
_ l(should be - 0 mg CaCOlL) l
- .2 0,11bti.1-06 Om I
12&:4 Z tl~l.3 i
(Si --7.05l
-X z l I
19I 54 1 oio3nlOO 4
Z I
I 103 3
4
-3,4 IA ftA~nvM1.
<,31.CLR W I
J.0
- 5. 1.4 tV/nI A4.0O
'I L1
-- I k,:1A
-wb/ 16 ot I 3
l 7
l_.-
112.1 l 3JL4 O
j I LJ4j Note: If>l5mloftitint is used. sample must be diluted.
Reviewed by:
Date reviewed l [
f-1I Z ZZI]
.=,
Environmental Testing Solutinn. LLC
%I e.-'.
.T,.,,
1-,
,ill...
S_, --
b 2,
111-1.
ii.; :
A -
Total Hardness (EPA Method 130.2)
~~
MamnxM Waier, MDL -10mg CaCO/IL -Kw
-Anay Date
~~~~~~~~~~~~~~~~~~~llnos
.~i,...-
- .etefta.
.Nos~ansu e~cdc
- Bqlo a-1.atM
- I
~
- 0) otS~rA 9
pXIFator orMuldplier
- nr; fetrece 3iS:m
.s-.II
.iS000!50mI l, number pie 0s.2) msaznple
.t Laboratory control standard.
standard number Tm volume Begin End Total Multiplier (mg CaCO/L)
(acceptable range (mg CACOIL)
(m1) ml ml ml 9otollO%)
11\\5:5 1/6 40 SO..
5 l
.4 l
O*
Dupicate sa lprecision:
Sample Hardness
%/&RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCOt1L)
((S - D) I((S+Dy2J) x 100 number (ml) ml ml ml (acceptable rnge -10%/)
Q tS~ L. Duplicate D
i }.
3 Matri sike recov_
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCo/L)
L(mlcocoV) ml) ml ml ml l
Sample hardness (B)
Measured spike value (MV)
% R = MV / SV s 100 j
(gm CaCOV/L)
M MV = A - B (acceptable range I
}
(me CaCO1IL) 75 to 125%)
Samole measurements:
Samplevolume Begin End Total j Hardness l Samnple number l Sample ED (ml) _
Jml lml lml lMultiplier l Me Cv)
Blank
-l I
(should be -0 mg CaCOsiU I
_l___
I I
6-1oc o SQ*1 2
l z
17lD.-41.
os3bI0.oS 3
1 3
.41
..Qa 1-7
-S-Is
=
5 IIlk 9 8.1
,(
o5 I
L
,_I I__
J 1-I-S
~
Note: If > 15ml of titrant is used, sample must be diluted.
Reviewed by:
De r e
Date reviewed I __ (0- I(, -aa
Environmental Testing Solutions. LLC Page 4 Page o
Total Residual Chlorine (EPA Metd 33.5
-~
Matrix: Watcr, MDL -0;.10 igdIL-i.
.. I*
~~~~~~MtrA iaM61AR2 pJMa Meter Dal analyzed
.Airagn Cali brat ion:
~~~~~~ ~~~~~0.10 tgL I
1.00 mrIL Not: Fr sinpa wth reidul clorne r ~1.0 rn&L, the calibration range must be adjusted to bracket the chlorine levels of the saM'Pls Laboratory control standard:
Refemncce standard True value (TV)
Measured value (MV)
% RS = MVI TV I 100 number (mglL (mgI'.)
(accepbable range - 90 to I110%)
IN-b-SIM0.50 Duplicate sample precision:
numrIC SapeIample characteristics Residual chlorine
%RPD = (S - D) [(S+D)/2])
100 number
~_______________
rnl)(acceptable matge -_10%)
_________{
Duplicate
.4 1 D
A..00jc~
S m m asurements:
Sample Samtplc ED SaMPlecbaracteristics Residual chlorine number (mglLI
~~4~ir%
Blank (should b = <0.10 m7fL)
-o________
L.!
.'-z
-ji M
a
/.)IO1
/1Cdkc.r a'J~o2ny O A
IAJ AC.00 2e3 Note:
ll saplcs were analy-zed in excess of EPA recomnuded holding time (15minutes) unlessother%%isc note:d.
Laboratory control standard.
Reference standard True value (TV)
Measured value (V)M
% RS = MV I(TV 100 number (mg/L)
(m l (acceptable range-0t10%
)A 5 3 0.50 I
I 4D. Ql Reviewed by Date reviewed L.Zg w--.--
Environmental Testing Solutions, LLC 5
Page Page ofI Total Residual Chlorine b ~ ~
~
~
~
~
~
~
(P Meh. 33:5
\\-
Matrix Water; MDL,0 mgL -
Re* Meterst.nd.rdnu14da pHton Mete 13r' Z5 Alyst [I Z]-
- .Žt tc~gql Date analyzed [3 5
- Acidrafg~y Calsration:
~~~~~~ ~~~~ 0.10 g/L I
1.00 ma/L Reference sandard number t5, CiI-p S 1 I
Note: For samples with a residual chlorine of 1.0 mgfL, the eslibration range must be adjusted to bracket the chlorine levels of the samples.
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = (S - D) I[(S+D)12Jl x 100 number j
(mg/L (acceptable range-1 10%)
0-)O".o 1 AF 1n f1 s k 0-003
_7*5_
Duplicate D*.ocoo.
° 0
Sample measurements:
Samplc Sample ID Sample characteristics Residual chlorine number (mg/L)
>k-.
e'Blank (should be <0.l0 eL)
'O*
,o"4 OC+/-3)oO).O0 3 &S1 VdY p-icy. \\\\C\\P\\
. (p,ly-<
<.O.o '
C>3tt09*ot;
)t 4
%;$E Sou
\\§ \\4totttA z P-1t
<~4O. a 3 t 63rO LF.oz
-Q z-'~-~
i 1
4wit A-lc.
(.oooZtZ
.~~~~~~~~~~~~.0 Xote: *A1J samples were analyzed in eccess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratorv control standard:
Refcrence standardl True value (TV) l leasured value (MV
% RS= LNIV/ TV s 100 numbe7 I
(metL)
I
(-CIL)
(2cccptable nnge-90t 1%
t415 134 1o 0.50 b.f2>41 i
Revieved by Datc reviewed EI i]
I.
Environmental Testing Solutions. LLC
-5f.o0i.
Page 5Z)
Page
- -.X,.:
rIf er Total Residual Chlorine (EPA Method 330.5)
Matrix: WaterMDL-O0.10 m Met. :
Accuimet Model AR2S pH/Ion Me Di/-analye s~~~~~~~~~~.
- .~
Date analyrzed
.3.
.~
.C.l.b.a.t.
Cclibration:
0.10 mgIL I
1.00 mz/L Reference standard number I
M>S 13 N h 61 q Note: For samples with a residual ciorine of > 1.0 mgL the calibration range must be adjusted to bracket the chloine levels orthe samples.
Laboratory control standard:
Reference standard Truc valuc (TV)
Measured value (V)
% RS = MV /TV X 100 number (mc/L)
(mg/L)
(acceptable rmnge-90 to 110%)
IN-6513 O.SOt 0
4 2.B I1.
Duli lcate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = ((S - D) I[(S+D)fl x 100 number tmclL)
(acceptable range -
10%)
sl, l Duplicate i
3 g
D 4.O00,oZ lZ -
Sample measurements:
Sample Sample D Sample characteristics Residual chlorine number (mct/L) iii7,.
Blank (should be= <O.lO m/L)
C
.00 Ololo~~bb 1 l~r no xa, ao
- waf3, o31b-ts it) i. 6A fT V
r-pJo diO.o03 0s1° L
oil. os
- CCL.Sti,'l
- te) C0L,°.,j o.OtL Oz1fill.13 ra A V)di rn YaCAClt-,
doa-c At
-A f
~(j l l
CIO#
{74LPK~
O o3 n R
+,
r]elu ZO.ou
,Vorc:
lsplcs vcre analyzed inexcessofEPArecontended hoidingtine(15 minites) unicotherisenoted.
Lab bdqrd Reference standard Truc value 4
vallue (MV) 0/0 RS = MV I TV 100 number (mglL)
(L) 9 to o%)
0.50 Revicved bl Date rvieNvced
Environmental Testing Solutions, LLC Page __of 3__
.tL st.
I.,
Alkalinity (EPA Method 310.1)
Matrix: Water ML = 1 mQ raCO3/L Date analyzed it). ji-b Titrate samples to pH 4.50 S.U.
_=ant no n
pHof Deloulzed water
- 4.5 S..
- e.
i...
Titrant reference
- number, Normality
'`check standard number Begin ml End nil Total ml I E
-.Wormilit () o HSO4 -'
' (5 ml aaCO OS.05)IE
- b.
gE 0./18..)
(aecceptable rnte - 0Q018 -.0QO22)
.* pH Factor or.Muitiptler
- (N z 50)l 100 ml saimple mN500 -Zn~Ar I
b C
I; 4.
4 l
Laboratoy con trol standard:
Reference True value Sample Alkalinity (MV)
%RS=MV/TVx 100 standard number
)
volume Begin End Total Multiplier (mg CCOi/L)
(acceptable rnge (me CaCO)
(m l
ml ml
- 90to110%)
(fDSi t6 100 100 ib-2 22..,
a 1 IbO j
Duplicate sample precision:
Sample Alkalinity
%/RPD Sample Sample I volume Begin End Total Multiplier (mg CaCOjIL)
((S -D) IRS+Dy21 11x00 number (ml) ml ml ml (acceptable range - t 10%)
j OO. b t l
_0
_ _ 2_
. Z Duplcat
.e 22.e_
D 5__-_
Matrix spike recoverv:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCOL)
(me CACOsVL) l m)lml lml lmll
,14j5 top
_Z_
10c-38A-It.2.
JD.2 It Sample alkalinity (B)
Measured spike value (MV)
% R =MV SV x 100 (mg CaCO3/L) lV
= A - B (acceptable range (mz CaCOVL)
- 75 to 125%)
Sample measurements:
t l ~~~~Sarnple volume lBegin lEnd lTotal ll Alkalinity l
Sample number Sample D ml ml Multiplier (me CaCO 4
f
_ I N,_C_
.34 I
1 40 4
.(.L 1 1.7 0.0 i
Reiewed by:
rl e 2
Date revicived: E1ZOflo 1
Environmental Testin2 Solutions. LLC Pagc_ - of_ s Environmental Testing Solutions. LLC Page 2 -er
'3 Alkalinity (EPA Method 310.1)
Matrix: Water, MDL - 1.0 mg CaCO3jL Titrate samples to pu =. 4.50 S.U..
-nb kr-Sli k15L I
l I
I
. Date anazed D. 1JO 3
, ru
-E 7..1.
7 _
nt*
- ~~~~~~~~~~~~~,
-tfJr t
I I
I Jl#UIJ&
USCD.)UJII S
DC t;L5)5f
- us~~ -
apz Sample Alkalinity
%RPD=
Sample Sample ID volume Begin End Total Multiplier (mc CacoilL)
((S -D) Il(S+D)121I s 100 number AL i
(ml) ml ml ml (aceptable rage
_0%)
03J.64J s6; C)DE I
S s~~~~zt7_ 0 N IC)l.t J,\\
i F wplicate 4
.%.g~
.4o t.U 2q. q I' Matrix spike recoverv:,
tXo J nI l
"Aap Rcference Spike value 7 Sample 5
Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mgCaCO3/L)
{me CJCOVL)
(ml) ml ml ml Sample al alinity (B)
Measured spike value (NMV)
% R MV / SV x 100 (mg CaCOV/L)
AIMV = A - B (acceptable range (mg CaCOVL)
- 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (me CaCO (a
11 Q L2 I~ +/- 5 L i I I J~ 2~
__I 03,Ono 1 0
.3Z331 6z 1
1 Oa4~~ ~
O 03, OQ.05 5
I.3 0
41 1 01 0.Oq q -
63403 ThU.O l
j R
io.
11-6 11050 lS 2L7 5'@.5 o?3iOO~.O W
Z.
q
-1 Re.iewed by:
Deriel C
Date revievcd:
~
,0l't
Environmental Testing Solutions, LLC Page 3 ?of Environmental Testing Solutions, LLC Page
of
'3 Alkalinity (EPA Method 310.1)
Matrix: Water, MDL 1.0 mg CaCO,/L Anayst I.
I Date aDyzed 1jD zj074 Titrate samples to pH = 4.50 S.U.
'.'F'
'3 v
n n
d t.,
.._t pH of orma Normality (A) of HzSO4 pH Factor or M Delonized Titrat
.check Begin End o
COjxO.O)/E rn(Ni 50 OOO 100 ml sample water reference standard m1l ml ml 0i
- -500
-4.5 &.
number number
(___ccepblers-_018_ _
22)_2 Laborator control standard:
Reference True value Sample Alkallnity(M
%RS-MVITVY 100 standard number (TV) volume Begn End Total Multiplier (te CICO)IL)
(acceptable range (mg CACO3 L)
(ml) ml ml ml
-90 to 110%)
100 100 2
01 C.5 10.2 q
Duplicate sample precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO1/L)
((S - D) /[(S+D)y21 1 100 number (ml) ml ml ml (acceptable rage -
- o/)
0_516Q7. 3Q 1b1 It }0 0 lore itM
.5 JC).2
(&t O
J Duplicate D
lko 3
i
.I Matrix spike recoverv:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume 1 Begin End Total Multiplier g(mg CaCO/L).
(me~COV)
§ ml)ffiml lml Iml I
-s o
b l 100 JlU,? '21;
- 1. 1 10).2 1 l3 Sample allalinity (B)
Measured spike value (MV)
% R = MV J SV 100
(-g CaCO9L)
MV = A - B l
(acceptable range I
l (me CaCO-IL)
- 75 to 125%)
50~~~1(
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier l
(mg CaCOjL) l o 0t q.03 l5L 1k) 2-
-10 VW l33-5 ( I l
Z l l bl.
l 3[-
33 U, I%9
'CO Z
1O-wbyo7oI Pfnd Ons &<
I IX s l A-4 IQ3 )0 O I 1 \\.8 12lO4.0T 1 I
}oll~~~o11 1
3 I
1 A~~2q.0tl~ l qq I
I I
Io I
p~.
I I_1____II_
o, i I
- 1.
q q I..
i..
i:.
t"..
Reviewed by:
I Dae r Date reviewed:l al I
Sequoyah Nuclear Plant Biomonitoring October 7 - 14, 2003 Appendix D Reference Toxicant Test and Control Chart 21
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromeis.
using Moderately Hard Synthticter
~~~~.
';?~'.:
'^~
-"r~'
} 1_,,
.1,
~~~~~~~~~~~~~~~.
1.0 0.9 0.8 0.7 0.6 0.5 0.4 1.0 0.9 0.8 I
I I
-S P-
- Y CO I
I I
L
~State and USEPAMadi Limits Set According to 4 2 Standard Deviations 1-'
0.7 0.6 0.5 0.4 USEPA Warning and Control Limits Limits Set According to 10th and 25'h Percentile CVs 0
I I
I I
I I
.1 Teso
'o~x'8ij,'4
'4'tV te ° Wu W4 Test date 7-day IC25 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- Central Tendency (mean IC25)
Warning Limits (mean IC25 SA10 )
Control Limits (mean IC25 +/- SA25 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Polassium Chloridc Chronic Reference Toxicant Control Chart for Pimephalespromelas using Modcralely lard! Synthetic Water State and tlSEPA laboratory Laboratory USEPA USEPA Test number Test date 7-day IC" CT S
Control Limits Sa tVrning Limits Su Controt Limits SP11 Wanin lmit i
S
- Cl limit CV (gQ.KCI)
(g/LKCI)
CT-2S C'r+2S CT-SA1l CT+SA.J#
CT-S.
CT+SA-u CT.S" CTtsA--;--
- s cTSs 1
10-15-02 0.66 2
10-22-02 0.64 0.65 0.01 0.62 0.67 0.03 0.57 0.72 0.14 0.51 0.73 0.25 0.40 0.9. '0.29 0.36 0.94 0.02 3
11-05-02 0.59 0.63 0.03 0.56 0.70 0.01 0.55 0.70 0.13 0.50 0.76 0.24 0.39 0.7
- 0.21.
0.35 0.91 0.06 4
12-03-02 0.61 0.62 0.03 0.56 0.68 0.07 0.55 0.70 0.13 0.49 0.75 0.24 0.39 O0t6 0.0.34 0.90 0.05 5
12-03-02 0.52 0.60 0.05 0.49 0.71 0.07 0.53 0.67 0.13 0.43 0.73 0.23 0.37 013 '-i.0.27,'.
. 0.33 Ot7 0.09 6
01-07-03 0.64 0.61 0.0'5 0.51 0.71 0.07 0.54 0.6S 0.13 0.48 0.74 0.23 0.33 O..
.27' 0.33 0.11 0.0 7
01-14-03 0.61 0.61 005 0.52 0.70 0.07 0.54 0.6t 0.13 0.43 0.74 0.23 0.31 014' 0.27.
0.33 0.t 0.01 B
02-04-03 0.64 0.61 0.04 0.52 0.70 0.07 0.54 0.69 0.13 0.41 0.74 0.23 0.33 0..4 r
Q21:;..34 0.39 0.07
- 9.
03-13-03 065 0.62 0.04 0.53 0.70 007 0.54 0.69 0.13 0.49 0.75 0.23 0.3t 035 -..It i0.23 0.34
.9 0.07 10 03-18-03 0.64 0.62 004 0.54 0.70 0.07 0.54 0.69 0.13 0.49 0.75 0.24 0.31 0.3S..p
.23 0.34 0.90 0.07 11 044B3-03 0.50 0.61 0.05 0.50 0.71 0.07 0.53 0.61 0.13 0.41 0.74 0.23 0.33 01 t
0.27; 0.33 al3 0.09 12 04-15-03 0.56 0.60 0.05 0.50 0.71 0.07 0.53 0.63 0.13 0.43 0.73 0.23 0.37 033.- -F 0.27:
0.33 Qt1 0.09 13 04-29-3 0.59 0.60 0.05 0.50 07 0.?
0.53 06
.3 04
.3 02
.703.'02; 03
.7 00 13 04-29-03 0.59 0.60 0.05 0.50 0.70 0.07 0.53 0.61 0.13 0.41 0.73 0.23 0.37 0.27 0.33 0.t7 0.01 14 05-29-03 0.64 0.61 0.05 0.51 0.70 0.07 0.53 0.68 0.13 0.43 0.73 0.23 0.31 0.27 0.33 all 0.01 35 06-1703 0.60 0.61 0.05 0.51 0.70 0.07 0.53 0.63 0.13 0.43 0.73 0.23 0.33 0.34
. 0.27 0.33 0.3 0.01 16 07-15-03 0.65 0.61 0.05 0.51 0.70 0.07 0.54 0.68 0.13 0.4g 0.74 0Q23 0Q3t
- Qt4.
CM(
oD:
33 Qtt1 O.Ot 17 0805.03 0.53 0.60 0.05 0.50 0.70 0.07 0.53 0.63 0.13 0.43 0.73 0.23 0.37 0323 ;
&.27
- 0.33 0.33 0.03 13 03-12-03 0.67 0.61 0.05 0.51 0.71 0.07 0.53 0.68 0.13 0.48 0.74 0.23 0.3t 14'.t%.> 027 0.33 0.3 0.0t 19 01-19-03 0.55 0.60 005 0.50 0.71 007 0.53 0.63 0.13 0.43 0.73 0.23 0.31 I :0.3
-tz0.27 0.33 0.33 0.01 20 10-07-03 0.61 0.61 0.05 0.51 0.70 0.07 0.53 0.63 0.13 0.41 0.73 0.23 0.31
.,. 0.14 V0.27 0.33 t0.1 0.03 Mote:
7-d ICu day 25% inhibition concentration. Anestimationofthe concentration ofpotassium chloride that would cause a 2S% reduction in Pimphales growth for e tt pop tladoa.
CT - Central tndency (mean IC5 j).
S - Standard deviation of the IC, values.
Laboratory Control and Warning U.imits
-}x'.
L:sboratoty control and warning limits were established using the standird deviation of the IC,, values corresponding to the Oth And 25th percenil CVL Tbese rs an o6Mts uidiasdiixetd m wu gEmits recommended by USEPA for the test method and endpoint.
t S.Lj-Standard deviation eorresponding to the 10' percentile CV. (SAt = 0.12)
S,"
Standard dcviation corresponding to the 25b percentile CV. (SA2l 0.21)
- j USEPA Control and Warning Limits S,,s - Standard deviation corresponding to the 75 percentile CV. ( SAl, 0.31)
SL, - Standard devUtion corrcsponding to the 90 percentile CV. (SG - 0.45) t.1
CV - Coefficient of variation of the IC3, values.
USEPA 2000. tnasurnysrAdAccoubigfx ethod VauibryinWhote EliucitTuxicyAplA.
ttdeiteNdtwi0Po1nliutXtwi e ElininiontPoraun EPA13 R.00-01 USEnvaou a
PrnoeAgeny.rku0L.,pr.i
,I Oa.3wismi ehamed Aquatic Bsvril.a. Inc.
'.i 1.4-Mak g.-:t 4!,; s
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pineph ales promelas using Moderately Hard Synthetic Water 1.0 0.9 0.8 0.7 0.6 0.5 I
.I
....................I.... I.....I... I.....................
I...............
I.
~ ~ ~ ~ ~ ~ ~~~~~~~~~.
~..
~
~~~~~~~~~~~~~~~~~-
0.4 0.3 0.2
,o
-US <'°'
'R
°%
° E °°
°R
°E
°°Te°>at Test date
.trlf 7-day IC 25= 25% inhibition concentration. An estimation of the concentration of potassium ch1oridd..>..s t.:
that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC 5)
Laboratory Warning Limits (mean IC +/- S S 1=0.12)
L a l i o r l o r y W r n i n g i m it s2 5
A.10 A.
Laboratory Control Limits (mean C25 + SA25' 5A252 0.21)
USEPA Warning Limits (mean IC25 +S A751 SA75 =0.38),
USEPA Control Limits (mean IC2, +/- SA.90. SA90= 0.45)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data
.fnr Pimpnhales t
mnAq -E using Moderately Hard Control
.Control Mean Test number Testdat Suvatl e-Growth 5
.K1--
- z
¢ t;C;
!:~~~~
i, 4
tor Control Growth (V)
(ngmg/ae) nslve Growt CV (%/.)
1 10-15-02 2
10-22-02 3
110502 4
12-03-02 5
12-03-02 6
01-07-03 7
01-14-03 8
02-04-03 9
03-18-03 10 03-18-03 1
0408-03 12 04-15-03 13 04-29-03 14 05-29-03 15 06-17-03 16 07-15-03 17 08-05-03 18 08-12-03 19 08-19-03 20 10-07-03 100 100 100 100 100 100 100 97.5 100 100 100 100 97.5 100 97.5 100 97.5 100 100 97.5 1.037 0.822 0.874 0.852 0.668 0.886 0.677 0.933 0.838 0.803 1.083 0.892 1.021 1.005 0.888 0.910 1.092 0.693 0.745 0.762 0.929 0.911 0.896 0.850 0.856 0.831 0.844 0.843 0.839 0.861 0.864 0.876 0.885 0.885 0.887 0.899 0.887 0.880 0.874 16.9.
10.6 2.8 9.1 10.4 4.1 3.0 14.1 8.0 21.3 6.1 17.0 6.7 7.9 4.2 6.7 6.5 9.7 2.9 20.0 13.8 10.1 9.9 10.0 9.0 8.1 8.9 8.8 10.0 9.7 10.3 10.0 9.9 9.5 9.3 9.2 9.2 8.8 9.4 0.23 0.13 0.12 0.12 0.15 0.14 0.07 0.15 0.15 0.21 0.09 0.17 0.18 0.11 0.18 0.12 0.13 0.20 0.08 0.18 21.9 15.6 13.8 13.7 22.4 15.7 11.0 16.5 18.5 26.5 8.0 18.7 17.5 11.1 20.7 13.7 11.8 28.2 10.7 23.1 18.7 17.1 16.2 17.5 17.2 16.3 16.3 16.6 17.6 16.7 16.9 16.9 16.5 16.8 16.6 16.3 17.0 166 17.0 Note:
CV - Coefficient of variation for control growth.
On average, the CV for control growth is 9.4% in Environmental Testing Solutions, Inc. Pimephales chronic toxicity tests.
Lower CV bound determined by USEPA (10$' percentile) - 3.5%.
Upper CV bound determined by USEPA (90'h percentile) 20%
NISD - Minimum Significant Difierence PNISD - Percent Minimum Significant Difference PNISD is a measure of test precision. The PSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Pimephales growth by 17.0% from the control.
Lower PMSD bound determined by USEPA (10h percentile) - 9.4%.
Upper PMSD bound determined by USEPA (90'e percentile) - 35%.
CT - Central Tendancy (mean Control Growth, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA.
000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency. Cincinnati, OH.
Organisms obtained from Aquatic BioSystems. Inc.
10-07-03.xls
Environmental Testing Solutions, Inc.
Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests 1,.
P; i...-
'l
- 1.t'§&- :§:l:'l,
0.,;
%`........0.w*
1.25
- i t M
- 0. -
0 0 o 1.00 0.75 0.50 0.25 0
Z P-5-
I -
'=0 C
I-CU 0
40 30 20 10 0
North Carolina Acceptance Limit (<40.0%)
Kentucky Acceptance Limit (< 30.0%)
I I
I I
I I
I I
I I
I I
I I
I I
I I
I 99 a,
0 a
0 a
4-V-1 V
-?
-'
',S
'Wa
'W4
'W",
"'j W'-
W?
Wa Test date
+
Control Growth or Coefficient of Variation (CV)
Central Tendency (mean Control Growth or CV)
Control Limits (mean Control Growth or CV +/- 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart;.
for Pimeplhales promelas using Moderately I-lard Synthetic Water 40 30 C-1 0-f 20 10 0
e...t Test date P-SD = percent minimum significant difference. PMSD is the minimum significant difference beLweeni the control ant treatment that can be declared statistically significant.
Ccntral Tendency (mean l'MSD)
Lower anti Upper PMISD Bounds LowerPMSI) 1ound (lO percentile) = 9.4%. UpperPMSD Bound (90tpercentile) 35%,e
&ti (Lower and upper IMSD bounds were determined by USEPA for the method and endpoin L
0*
.
I..'
I
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification or Data Entry, Calculations, and Statistical Analyses Test number:
PpKCICR 56 Tet dates:
October 7.14, 2003 Raw~~~~~~~~wal 1..
CRJusik-a (/L KcCt
.&ct.
IsW_ 6t*1,1Otat of 1a..bera t
A -
r.sw t -
'tah
- t Laa.9.1ight(bt)
W 1141
.sa..
lsar s-nt M.. weght (.)
cat d
t""
rte ic_" tf.
Ka) lraue arva.
(wt)
Cfbl (gw)
A.11 of B.Tirs()
%m M
- 4. -
A 10 10 15.114 22770 7656 07656 B
10 to IS 124 24380 9256 09256
.2
.8a e
Control C
10 10 15.106 23 070 7 964 4
- 97.
0.7616 07926.0 4
N plile D
10 9
15233 20820 5.587 0.5587 E
to 10 15 242 22 70 7 628 0 7628 300 F
I0 Is05 22 540 7 32 0.7382 100.0 0.31 1
G 10 10 15.187 24 30 9643 09643 I
I I 0 1
O 25 262 23 050 7.788 0 7788 I
I 1
a 15 292 21 250 5 958 0 5953 410 I 0 1
0 152764 228$30 7 566 0 7566 900 0.7136 I5..6.$~
- 3 450 10 9
15 249 21.780 6 531 0 6S31
- 1.
10 9
15.263 23 750 84S7 08487 M
10 a
I 057 20 00 5.743 0 5743 600 N
20 3
15023 20180 53$57 0 5857 0
10 9
14990 21.340 6350 06350 t7.5 0.6069 5
20 10 1____
20 15005 21330 6325 06325 r
10 5
15 056 2S 660 3 604 0 3604
~~so R tO
___t 6
Is5020 19 170 42150 041506.0.4327
~
- 3.,
S 1 0 7
2 02 18.500 3.438 03433 i
T 0
i 14836 20980 6094 06094 p
.00 IU 10 1
14 946 15.760 0
14 00314 V
I0 0
0000 0000 0000 00000
- s.
0.023 123 ii IV 20 I
0 0000 0000 0000 00000 e...
X 10 I
14951 25430 0479 00479 unnett's I)SD value:
0.1762 MSD Minimm Significant D1ffrce PMSD:
23.1 PMSD Percent MinimunSignificant Diffriene PMSD is a rnaswe of test precision. le PMSD is the inimn perant diffence k
b the i
udthitEs ied stutisticallysigificsntina wholecMLuttoxicitytest. Onsavagea aipsficanltdiffer e aetonfrfEnwor=aTehSosijonhe.
chronic toxicity tsts wen i toxicant reduces Pincspala growth by 17.0Y fior the control (ddervned drcitretatxicnrluqin I owsr PMSI) bound dctined by USEPA(10th pacuntile) -94%
tLpsr lMSD bound detentincd by tJSEPA (90th pctentilt) -35%
- .i
.C g
The lower and upper bounds were calculated by the tJSEPA using 205 test eonted frtu 19 lhoiteat fr P ae t
in chronic rerence toxicant tests.
USUIA 2W) 1.tsdrstanxiing and Atcounting Gr M thud Vuriability n Whole ElIluent loxicity Applications tkdr the Njtional Pollutant Discharge Elinfnation Program EPA233-R40.001..
ud Pbtt A&ency. Cincinnati. Olt
.~~~~~I.
Orgarnmnss obtasnedfrom Aquatic US)'ters. Inc.
1007.03 x s
, ;,.sJj;,
- ,: ~-f
';t.
1.-
I I.
Environmental Testing Soutions Inc.
Page of S
Species: Piniphales promels
,,.-m,'g'f'~~~~~~~~~~~~~~~~~~~~~~:
ffi~~~~~~~~
*4'i t-
-@2V<--
Dilution preparation Information:
- Comments
.r..,.
KCI CHM number:
CAR0a1 Stock preparation:.
0 KCU Dinolve 50 g KCl in IL Daonized ata Dilution prep (mgtL) 300 450 750 9
Stock volume mL) 6 9
12 15 IS Diluent volume (mL) 994 991 9ii 9i5 932 Tot volume (L) 1000 1000 100 0
100 Test organism information:
Test information:
Organism age:
25 IC)
$I
-M 7
-tS Randomizing template: k Date and times organisms Jt-Z
-rm N1 H Ur Incubator number and 3 F1 wvere born between:
shelf location:
Organism source:
A&S IQ 1O6a Artemia lot number:
B&*f1j Transfer bowl information:
pH = TR2SU Temperature =X.N1 °C Total drying time:
MAAS.
Date/Timein: 114.,
I5c Average transfer volume:
Date/Timeout: j-i jjoS ok cs
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _O ven tem perature:
l tO C.
Daily feeding and renewal information:
Day Date Morning Afternoon Test initiation, Control water Analyst feeding feeding renewal, or batch used time time termination time 0
io-oia-o.
0 l0 10.0(o.b3 IO"oR-o 3 oRob is cO 10________
2 oiA-Ci?
OzS5 Is Lo aB03
<1l 3
Jo-il-'J o
S oC 136-os-n A L 4
10' l-CJ3 C 3~s j5j IDO-o-5 6
jt G36a 12-- 3 j 'sot 13OSC
____1-_
)OQ)C is1 00 6
Io 3ob 1O o8-65
{
7 loqq -bHi 1589 1 rj Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
2-.' 5V S20%
7-day LC50
_7P_2. _
Average weight per initial larvae:
NO.
7E.I-t1 NOEC
(
o Average weight per surviving larvae:
7M I 2 0.25 mgeiarzva LOEC 15 B ChV (01 b -P ICII (ol.k
Environmental Testing Solutions, Inc.
Page 2 of S Species: Pimephalespromelas
- PpKCICR Test Number: jo
-r
!'i.:,-
Day.
l Day b
- s f -
.. C" I r :
L' 0
A D Z) I 1zD I / D I/o
/
/) I /0 I /
I
/0 Il;I 10 I
l lo
/1 -
I l
lo I0lo I t o I loI to o. 1h 1.1/ I o to I )
I I
3 to_
0 to lO
/L
/0
- 1) iL s c)
J() /
1(
10 10 10 IC
/
IL) I 9
/0/0 /
l lo /0
/0 lo 10 9
q 77________
_ /0
{O Id 5
q 15 /0
/0
/
o to A Pan weight (m)
,_\\
-§ B - Pan + Larvae weight (mg) t C-Larvatweight (mZ)
-A-B
~4
~
~
~
eo"c~;'~
Weight per lnit Wi number of i
-C/lnitial numberorlrva e 0
a o
o-Average weight per Initial number orlarvae (mg)
.L o0.
elIC) 0.*Ja Calculations and data reviewed: A _
Comments:
Environmental Testing Solutions, Inc.
Page 3 of
.. ~ ~ ~ ~ ~
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~N Species: Pimephalespromelas
'PKCRTest Number.
- a ~
- .~
~. -
6 0 0 KCUL" l
b't.L-4900 mR
- t. l Mw 3-6 O
- DI/DIIC'I/D I}O I}D L}° LD 1/D 1;O
/D 1FO 1 9~~~~~~
B M
Sury0al and Gr_
_zh Data Z
Day
~
00 me XCUL
~
-70mRKC/L'
~
00 m R M
N 0
P Q
R S
- 'T -
- W W
-X 0
D
/O 10 /0 0
O C
/
O
/
/Z t77 IC
-O O IC A 62A Iq IV B
Pim
+-l~rvae weight (md (0/0.0 o
4 4
6 ~~~~
c~~1~ I) 1 2-0~
2..
A - Pan weight (me)
B - Pan + Larv2e weight (mg)
,~~~'
Larvae weght mg)A - B f'x
~ ~ ~ ~ ~ ~ ~ ~ ~
L\\'.
Ob A_
WVeight per initial number of
-A larvae (mg)
I
-CIntal numnber oflarvae O
0!;0
'!O0 Average weight per WIl numberotlrvae(m)
- i.
06 (3.LI3,-
O. 6 33 Calculations and data reviewed.
Commehts:
Environinental Testing Solutions, Inc.
Statistical Analyses Lara rub Cw h
dS S
A T S l7 Dayw Swlm
- ISt E
naxTba 3;Typc ttr da
?uOMPINIMC.
- 1110" 300
- 1.0000 2.06 1.4120 1.4120 1.4130 0.000 4
. 20.00 10.00 0
40 450 09000 0.9231 12.23 2.1071 1.4120 9935 4
1350 O.00 4
40 600 01750 01974 1.213 1.1071 1.4120 i"6 4
1100 10.00 j
40
- 7 0.65.o 06667 0.9424 0.7S4 2.1072 14670 4
20.00 20.00 14 40 7900 0.050 0.0513 0.2403 0.15 03218 39.161 4
20.00 10.00 33 40 AerlimTelts Sbtisic Clftimi Sl Ks" Sapuao-W&I Tesk C ata n "duoatu 0.01)Masiot
.p........
0.9S5t84 0.84 1216894366
.0.4492.3959 Equality ofiitnce cnnott eontmed Hypeh.s Test l.tlL@.05)
NOEC LOEC CbV TU Steels MUayOnc RsnkTest 600 750 670.3203932 Tteanuts vs D-Cotrol Maximum L IUhobrd"Probin Parmrer Val" SE 95% FIduciti Llmits Contrl Chl.S4 Crika P.wlue Mu 1CiMA Iter Slope
.26.2132114 5.116792651 16.18990733 36.24773493 0.025 6.273453s2 7.314724922 0.2 2393552263 003814054 12 Intepi
.70.3655292 14.469562 9.95932932 1
.7777652 TSCR 006169346 001914797) 00241593153 0099219374 1.0 Point Probits
,mgL 95% Fldadal Limits 09 ECOI 2.674 638.0050228 532.9614929 682.428962 ECo5 3.35S 6T.3554925 607.9715615 714.1070396 03 ECIO 3.718 699.35564 639034736 72068026 0.7 ECIS 3.964 714.5327431 660677919 744.771476 EC2o 4.158 7261626868 673.0947615 735.312716 o.s EC25 4.326 737.6101076 693.1451141 764.7678779 20 EC40 4.747.65.4017799 730 824S 790.991142S EC50 5.000 722.6223106 7525t27038 309 223472 6 0.4 EC60 5.253 800.2304271 772.9876295 830.0188392 3
EC75 5.674 83038102 803.4198929 30.4613713 ECSO 5.542 42.6595601 14.4996111 3390674917 02 ECsS 6.036 357.2004598 826.9364588 91.-c8a2S94 0.1 EC90 6.2s2 875.8532105 842.097986 941.395078 0
EC9S 6.64s 904 2486406 664.0535856 988.694212 I
10 100 1002 10000 EC"9 7316 9O6020341 9049241604 086 12777 Dose mgfL DPs.RespR am Flt OrantmS obtarnsdrom Aquatic BiSysmei Ic3 1"37403.xds
Environmental Testing Solutions, Inc.
Statistical Analyses Lami Va C wn a" Sww1mr Tso7 D" Cqwa-st DMZ. LoflO3 Toga.
. PpKCAl am ftEF48"Tmcat IM W
I WI 4 Lal tD... m Eff.TtiigSalidrf smhtrypc
- .;- ;b;sb-limDwor d
Samp Dw hatocoa CHIO6C "EPA-42.I03413)
St 5pedau fPksp *h s pe s
Cafe.esoL 8
2 3
4 DC 4nbol
- 0.7636 1.0000 0.764t4 600 0.6069 Q
3<
900 0.014 t2<
11O1D
.0000 O20 Transfoms Vasfouwd I-Tau"dJaht CeaenmtfL Mesa N-Mes Mesan MI Max CVh N
I.Stat Citial MSD Messa N-Mesr D-CowskoI 0.7616 1.000 0.7616 0URI8 0.936 19.936 4
0.71163 1.0000 300 0.2110 1.064 013110 0.7212 0.9643 11.766 4
.0.643 2.290 0.1762 0.7:63 1.0000 450 0.7136 0.9369 0.7136 0.5951 0.1417 13.697 4
0.624 2.290 0.1762 0.7136 0.9073 600 0.6069 0.799 0.6069 0.S743 010 1173 A
2.0111 2.290 0.1712 0.6069 0.7713 750 0.4334 0.5691 0.4331 0.3438 0.6094 27.810 4
0.4334 0.J312 900 0.0323 0 0424 0.0323 0.000 0.8824 122.977 4
0 0323 0.0411 Auxilla,,y Teets Sta1Ut5c Crillal 3k&.
Ki Shasp sTW s Teat dat n ora l dstnbuon (p > 0.01) 0.959667146 0.4 0
4
.01122412
.23622648 artersl Test iditesequal vrnces (p 01
£9
<74640446 11.3448201 0Itp.teits, Test (I.1.0.05)
NOEC LOEC ChV TU MSDu MSDP M5S MOSE F-Pr.l 6l Dltunts Test 600
>600 O.t7617S97 0.31331013 0.0304139t2 0.1837293 0.103036903 3.12 Trestnmets vs D-Control Linear Interpatlen (200 Iesamples)
Plint umfi.L SD 95% CL(Elp) 5kw IC05 381.06 t09.13 0.00 562.43
.07379 ICIO 457.27 12.38 76 11 587.18
.0.7464 ICIS 513.33 70.26 242.6a 649.79
.0.5336 1.0 IC20 8.a3 66.23 306.95 683.05
.0.6203 0.9 IC25 614.33 35.16 344.12 131.24
.0.1017 0.0 IC40 71631 3400 615.30 802.81 0.0270 IC50 76505 2192 67792 30932 4.5590
/
0 6
-0.5 S.0.4
- 0.3 0.2 0.1 0.0
.01
.0 2 0
200 400 600 a
1000 Dose mgL Do.eResponse Plot 1-ta. 0.05 level of signdicance Organismn obbainedfrom Aquatic BiaSystems. Inc.
1.70 i
10.07il3.ds
I:
niomna etn ouinIc Pagec40ofS Environmental Testing Solutions, Inc.
Page 4 o
Species: Pimephales Daily Chemistiy:
promelas
.-q.; PPKCICR Ten Number iki'
- S
.-UN~i;\\*XA&.:SS
.0).-
§ 1 w-'tl~a-5ts'tu
- AN~e r,!? *.4 j A.a5..
I Concentration I Parameter CONTROL OE (S.U.)
DO (mgL)
Conductivity (gtmhos/cm)
Alkalinity (mg CaCOt/L)
.~~~
Hardness (mg CaCOIL)
Temperature (QC) pH (S.U.)
8___q j
DO (mg/L) 183 300 mg KCI/L Conductivity (jimhos/cm) q Temperature
.b Z5.2 ZS*5 Z% *
- t.
Z 4.1 pH (S.U.)
1.0 5078-wS; gg
,n DO (mg/L) 883 8.3 I8.
450 mg KC/L Conductivity o q_
(ijmhoslcm)
________C goO 1_
Temperature v-s.
Z 2b COC) a______S' Z__
ZT___
7A 4
-A
___q pH (S.U.)
3.03 4_
l
- s-f s
DO (mg/L) l1 8D0 8
.3 600 mg KCI/L Conductivity (tmhos/cm) 1360 zgo 1
302 Temperature (IC)
Z 5.Z
_ _z_._
pH (S.U.)
a4I
.uGq 8
Li
-8 DO (mg/L) 3-
- l Z.
oq.c 750 mg KCl/L Conductivity ioo
(+/-mhos/cm) 1__3___
l Temperature I.
qq LN.
C C) 0 q
PRl (S.U.)
i3 1.)Z lIO 85 _
P
}-os
}
DO (mg/L)
_._q __
900 mg KCIIL Conductivity I W1'O (limhos/cm)
Temperature e c (0c)
^,
SZ Z.
Yt
- 2.
4t STOCK Conductivity 11_
(pmhoslcm) 1 l
Initial Final Initial Final IInitial Final Initial Final Initial I Initial Final
Environrmental Testing Solutions, Inc.
Page f S Environmental Tcstin Solutions. Inc.
Page 5 of S I
Species: Pimephalespromelas
- ,PpKCCICR Test Number.
J10
.:.X
-'...;.;X i -.
- -'t
- tto;'JN
- .s
.. I.
h,,% ",
Analyst I U
Concentration Parameter *'¢3~
CONTROL 0H (S.U.)
_O (mg/lL) -- "
d Conductivity
(,Mhos<;s) 267',
Alkalinity (mg CaCO]/L)
F Hardness (mg CaCO3/L)
\\
Temperature CC)
I I
I pH (S.U.)
S1 95
-2 'X2
.6
.o 9~
Z DO (mg/L)
I
.e.o
(.8 300 mg KCIL Conductivity ei9 (jimhos/cmn)
Z 824 Temperature ZL4A 2t*.'
ZSz ZSf ZS.
Z.6 Z.3 24 (QC )
pH (S.U.)
-4
.6 o
.26 DO (mg/L)
B 10.2 S.
-,+
.0 8o tA 450 mg KCIIL Conductivity (gmhoslcm) lg 3C)
_I3 131o b Temperature Iq pH (S.U.)
213 tq o
Z 1?1 1.&>
1L.32 600 mg KCI/L DO (mgfL)
'Z LP3 3o 1
Conductivity I
IM1310 13q40 Temperature
- 5. 2 2*
2U (OC) zf l
S pH (S.U.).
=t35 750 mg KC/L
, a_ _ f w_ ~~~~~~~~~~~~-
.9
-_-.,.if
_-I DO (mg/L)
'.0 2+/-L (Z..e 8.0 0,
Conductivity lNI(M (gmhos/cm)
_1(__
15:1 i(wZ Temperature (C)
Z.5 Z7.
1 Zq.e 2S4s ZS.3 I
V I
_9-9 pR (S.U.)
-. es DO (mgal) 900 mg KCVJL Conductivity (umhos/cm)
I SO 1; 4. 7 lq0°
- 4.q~ I 2.,%
-3.q...._k~
V 8--0 INWc 16(oO Temperature
(°C) 2q.1 ZS.I
-ZS.
74.19 STOCK Conductivity (mhos/cm) 22l
_=
>3BOO l_ -- -
16 1
7Z5 3-J~~~~~~~~~~~~~
P1ON Initial I Final I Initial Final Initial Final Initial Final I
I
-
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dbia.'*"
using Moderately Hard Synthetic Water
- Adns 1
1.15 20 E.
f II4I I
U z
tn Sac U
1.10 1.05 1.00 0.95 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 State and UyEPA Manated control Limits Limits Set According to A 2 Standard Deviations Laboratory Warning and Control Limits Limits Set According to 10 th and 25 Percentile CVs Test date 7-day IC2. = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 L SA.10 )
Control Limits (mean IC25 S
or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately fiard Synthetic Water State and USEI'A Laboratory Laboratory USEPA USEPA Test number Test date 7-day IC23 CT S
Control Limits SAls Warning Limits SAnS Control Limits SAM5 Warning Limits SA.
Contra Limts CV (gL NaCI)
(gl. NaCI)
CT-2S CT+2S CT Sl CT + SAAO CT SAs CT + Sjs CT S...1 C+
CT S-1 08-6-02 1.05 2
09-04-02 1.05 1.05 0.00 1.05 1.06 0.08 0.97 1.14 0.18 0.7 1.23 0.47 0.58 1.53 0.65" 0.40 1.71 0.00 3
10-08-02 1.03 1.04 0.02 1.01 1.07 0.08 0.96 1.13 0.18.
0.87 1.22 0.47 0.57 1.5 065 0.40 1.69 0.01 4
11-0502 1.03 1.04 0.01 1.02 1.07 0.08 0.96 1.13 0.28 0.86 1.22 0.47 0.57 1.5t 0.65w 0.40 1.69 0.01 5
1203-02 1.02 1.04 0.02 1.00 1.07 0.08 0.95 1.12 0.28 0.86 1.21 0.47 0.57 1.50
.!0.64 0.39 1.68 0.02-6 12-03-02 1.03 1.04 0.01 1 01 1.06 0.08 0.95 1.12 0.28 0.86 1.21 0.47 0.57 1.50 64r-!.
039 1.68 0.01 7
12.04-02 1.02 1.03 0.02 1.00 1.06 0.08 0.95 1.12 0.18 0.86 1.21 0.46 0.57 1.50.
064v'-
0.39 1.67 0.01 8
12-06-02 1.03 1.03 0.01 1.00 1.06 0.08 0.95 1.12 0.18 0.86 1.21 0.46 0.57 1.50; 0.64 0.39 1.67 0.01 9
12-11-02 1.04 1.03 0.01 1.01 1.06 0.08 0.95 1.12 0.18 0.86 1.21 0.46 0.57 150 0
0.39 1.67 0.01 20 12-18-2 1.04 1.03 0.01 1.01 1.06 0.08 0.95 1.12 0.18 0.86 1.21 0.47 0.57
- 1so, 064Kv 0.39 1.63 0.01 1
01-07-03 0.96 1.03 0.02 0.98 1.08 0.08 0.95 1.11 0.17 0.85 1.20 0.46 0.57 1.49 0.64'--:.
039 1.66 0.02 12 02-04-03 0.99 1.02 0.03 0.97 1.08 0.08 0.94 1.11 0.17 0.85 1.20 0.46 0.56 1.49 J 0.64 0.39 1.66 0.03 13 03-053 1.05 1.03 0.03 0.97 1.08 0.08 0.94 1.11 0.17 0.85 1.20 0.46 0.56 149..
0.64 0.39 1.66 0.03 14 04408-03 1.03 1.03 0.03 0.98 1.08 008 0.94 1.11 0.17 0.85 1.20 0.46 0.56 1.49 0.64 0.39 1.66 0.02 5
05-06-03 1.05 1.03 0.02 0.98 1.08 0.08 0.95 1.11 0.17 0.85 1.20 0.46 0.57 1.49 0 064; ;
0.39 1.66 0.02 16 06-04-03 1.07 1.03 0.03 0.98 1.08 0.08 0.95 1.11 0.18 0.86 1.21 0.46 0.57 149 0 64' 0.39 1.67 0.03 17 07-08-03 1.03 1.03 0.03 0.98 1.08 0.08 0.95 1.11 0.18 0.86 1.21 0.46 0.57 1.49 :jQ-64
.. 039 1.67 0.02 18 08-05-03
.04 1.03 0.02 0.98 1.08 0.08 0.95 1.11 0.18 0.86 1.21 0.46 0.57 1.49
.,Z,.0.6<:6 0
-39 1.67 0.02 19 09-10-03 1.05 1.03 0.02 0.98 1.08 0.08 0.95 1.11 0.18 0.86 1.21 0646 1.0 0...
0.39 1.67 0.02 20 10-07-03 1.02 1.03 0.02 0.9834 I.0S 0.08 0.95 111 0.18 0.86 1.21 0.46 0.57 1.49 :
064 039 1.67 0.02 Note:
7-d IC day 25% inhibition concentration. An estimation of thc concentration of sodium chloride that would cause a 25% reduction in Ccriodaphnia repuoduction forthc te poaslsas.
CT - Central tendency (mean IC2,).
S - Standard deviation of the IC21 values.
ju-Laboratory Control and Warning Limits t
g u
Laboratory control and warning limits were established using the standard deviation ofthe IC2, values corresponding to the 10th and 25th percentilc CV.
Theserang amtn atit trol ad ting limits recommended by USEPA for the test mcthod and endpoint.
S&t. - Standard deviation conesponding to the10 percentile CV. (SAlo - 0.08) t SA.25 Standard deviation conresponding to the 25"' percentile CV. (SA2, -0.17)
'..r USEPA Control and Warning Limits
= Standard deviation corresponding to the 75"' percentile CV. (SA7 0.45)
J SA.
Standard deviation coaucsponding to the 90" percentile CV. (SASI G 0.62) r CV I Coefficient of viriation of the IC2, values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Efflucnt Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-S33-R-
- 03. US r
P n Ae.y CninmatL Oil ty,~~~~~~~~~~~~~~~~~~~~
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodlaphnia ditbia using Moderately Hard Synthetic Water 1.8 1.6 I
I i
I I
I I
I I
I I
I I
I I
I I
I I
I - - -
0-.
U 0
N 1.4 1.2 1.0 0.8 0.6 0.4 0.2 I
I I
I I
I I
I I
I.
~
IL
~
I
'0%
toP
'0 "l
1Z.
9o0 0
all Test dated 7-day C25 -25% inhibition concentration. An estimation of the concentration ofsodium chidndlthaWbud iuse a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
..- Laboratory Warning Limits (mean IC25 : SA10 SAIO= 0.08)
Laboratory Control Limits (mean IC25 I SA25, SA2
- 0. 7)
.1 S
USEPA Warning Limits (mean IC25 +/- SA.75 SA75 = 0.45)
USEPA Control Limits (mean IC25 +/- SA90 SA.90 = 0.62)
- 4
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements
~~~*
.;:S Sodium Chloride Chronic Reference o
i Data..
lfoieiodap~nia-dbi~~~
- using ModeratelyHard Tast Control Controles can' Test date
- r C
nuTber Survival Reproduction Cr C_
se for Control Mean for Control
(%)
(ofmpringtemale)
Reproduction
(/)
Reproduction 1
08-06-02 2
09-04-02 3
10-08-02 4
11-05-02 5
12-03-02 6
12-03-02 7
12-04-02 8
12-06-02 9
12-11-02 10 12-18-02 11 01-07-03 12 02-4-03 13 03-05-03 14 04-11-03 15 05-06-03 16 06-04-03 17 07-08-03 18 08-05-03 19 09-10-03 20 10-07-03 100 100 100 100 90 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 28.4 31.4 31.1 29.5 34.0 33.2 32.5 29.7 33.8 30.5 33.2 32.3 28.7 26.3 27.6 25.9 29.0 33.3 29.3 33.4 29.9 30.3 30.1 30.9 31.3 31.4 31.2 31.5 31.4 31.6 31.6 31.4 31.0 30.8 30.5 30.4 30.6 30.5 30.7 8.0 10.4
- 6.7 9.2 8.0 6.2 6.0 11.0 13.7 7.4 7.0 8.1 5.1 6.2 10.8 5.9 11.6 6.6 4.3 8.0
'9.2 8.4 8.6 8.5 8.1 7.8 8.2 8.8 8.7 8.5 8.5 8.2 8.1 8.2 8.1 8.3 8.2 8.0 8.0 2.7 9.5 3.0 9.5 2.9 9.4 2.5 8.4 2.7 8.0 3.3 9.9 3.2 9.8 3.0 10.0 2.9 8.5 2.9 9.4 2.9 8.6 2.7 8.4 3.5 12.1 2.5 9.6 3.2 11.5 2.6 10.1 3.2 10.9 4.7 14.1 3.1 10.7 3.2 9.5 9.5 9.5 9.2 8.9 9.1 9.2 9.3 9.2 9.2 9.2 9.1 9.3 9.4 9.5 9.5 9.6 9.9 9.9 9.9 Note:
CV - Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 8.0% in Envirornental Testing Solutions, Inc. Ceriodaphnia chronic toxicity tests.
Lower CV bound determined by USEPA (10 h percentile) - 8.9%/.
Upper CV bound determined by USEPA (90' percentile) - 42%
MISD - Minimum Significant Difference Pf*SD - Percent Minimum Significant Difference PMSD is a measure of est precision. The PSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.9%
from the control.
Lower PMSD bound determined by USEPA (1 0 h percentile) - 11%.
Upper PNMSD bound determined by USEPA (9&d1 percentile) - 37%fo.
CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
1O-14-03.Xir
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Te
'r-.
35 30
.0 (I
w l aO Q W
=
9 r.
o 25 20
_.. _.~
~..
Minimum Acceptance Criteria (15.0 offspring per surviving female)
F I
I I
I I
I I
I I
I I
I I
I I
F I
F I
15
=
-2
=
- ! 0
_C L
0 Qc CZ C
40 30 20 10 I I I
I I
I I
I I
I I
I I
I I
I I
I I
fi North Carolina Acceptance Limit (< 40.0%)
Kentucky Acceptance Limit (< 30.0%)
0 1/2 0 5, 2, aOS O 10, Test date
+
Control Reproduction or Coefficient of Variation (CV)
Central Tendency (mean Control Reproduction or CV)
.Control Limits (mean Control Reproduction or CV 4 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 50 45 40 35 30 25 20 15 10 5
0
%o Vo a0)
°£t
°S 0
v 2'os
-,o
.t'
°V0% °)
°0 0t o
Test date PMSD = percent minimum significant difference. PMSD is the minimum significant d between the control and treatment that can be declared statistically significant.
- Central Tendency (mean PMSD)
Low...
nver and Upper PMSD Bounds Lower PMSD Bound (10"' percentile) 11%, Upper PMSD Bound (90"' percentile) -
(Lower and upper PMSI) bounds were determined by USEPA for the method and endj
.1a~z z
vs~s I E.vironmental Testing Solutionsnc.
. Page of 6 Sodium Chloride Chronic Reference Tosicant Test (EPAZ821-R-02-013 Method 1002.0)
)
.. x 'Species: Cerioaphniadubia :
- i.,
J-...:L.,
~.daC R,r,,U:; so~t
- ,-n',,.J2,,j.
-se-..z4X,
- 4 5fi
^ ';,
- 5 &,X,,,_-',!,'t dwS S '$
Dilution preparation information:
_l_Comments:_
l NaCI CHM number.
I C__
________1 Stock preparationr.
100 N&CV1 (dissolve 50 g NaCI in 500 m deioized water)
Dilution rp (mL) I600 go800 1000 1200 1400 Stock vlumc (mL)0
(
-i 021 Diluet volume (mL) i 1491 148S 1435 1482 1479 Totalvolume (mL) 1500 1500 1500 1500 1500 Test organism information:
Test information:
Organism age:
< 24-hours old Randomizing template:
Date and times organisms
/0*01-03 0?5Z ;O Incubator number and C Z were born between:
11 (
shelf location:
I _
Organism source:
Q S0 2
A-P)
YCT batch:
ABS fV-1110 Transfer bowl information:
pH =
SU Temperature VE1APC Selenastrum batch: ABS I 03 Daily renewal information:
Day Datc Test initiation, Control water batch Analyst renewal, or used termination time 0
_o n
I 2
I osq Ol 5 3__
l0lQO Ql-1o*d-a3
\\
l o i I 0 5
1 0 M
lo-oa-as 6
,o-13*03 I____ _o_
_t_
Control information:
Acceptance criteria Summary of t st endpoints:
% of Male Adults:
a; S20.%
7-day LC50
> itl&~
% Adults having Yd4 Broods:
J%2 so0%
NOEC Noo
% Mortaliry:
O 20%
LOEC Mean Offspringfemale:
2! 15.0 offspingrcmale C2S R
.4
% CV:
<.o. 40.0 %
1C25
Environmental Testing Solutions, Inc.
Page 2 of 6 Species: Ceriodaphr CONTROL ua dubia Survival an I.
._plicatc~~plea number -IN4.'^$.43&;
D a y 4
' "~~~~ '~ 1 ~ ~~ 2 ~ *
" 3 ~ ~ ~
~
~ 6 '?~~ it~~ i7zU i 8~ ~ ~
11 0
. 1
~.,. Yowgprodueed.
,Q.
Q. *0 a
(O T7Q
- 02o zi ;
- Ad u lt m o r ta l t y ~ >q. ; "
4 t
l
' L n J
<3 U : 2 ~ X : z L 4 I Z.'
DA-4 tr f,
,4'
.r f
V
-9 3
Young produced
-O
_ Jidult noftatY It L
- s_'"
2 Young produced O
Q
.0
_O_
Adult mortality L
l I
S Youngproduced I
3 2
ll io 1
iz.
l l
o
. Adult mortallty L
i
- 7 j Young produced t
C, l O O
_O Adult mortallty lk k
I L
l F
W L W..t Ad
.tnmora-
-y-
_~*
T._
7 J Young produced 1L L
1aI1 1
A I(
11 Z
+/-
l L6
' 16 Totalyoungproduced 3S If 3.
z129 31 a 's 31 153 l I
FtnalAdult Mortalitv L
CZ
=
l C
_l Xfor3' Broods I '
>C l
> z-l c
Concentration:
% Mortality:
l Mean OffspringFemale:
-S3,A j
600 mg NaCI/L Survival and Reproduction Data I
RcpUCate number I
Dyl I
2 1
3 1
4 1
5 1
6 7
1 8
9 10 I
Young produced
° l
CD
( )
° l
I l
)
i g
4
~Adult mortaUty L
L
_ltl6l(l-L-1 Ll1 2 j Young produced
)
C) l l-
=
=Adultmortalty k_
L-3 Young produced O
j 3
j) j)
j Cj IAdult mortality l
+ L Ll L
L L L 4
lYoung produccd l1 l2...
'A l__ (_
S5 S1
__IAdult mortality L
L-S
! Youngproduced S 1 I1/
1 lo 1
I
____l Adult mortality L-
.1 1
lL L-It_
l tl
__l..
r l Young produced IW O
I fl Fl n Adlt Adult mortality l
L I
l 1
l L iTotalyoun produced l
-l 5
5(,, l S -5 3Lk 3
0 3
lO FtnalAdult.N_
_rtalitv I
I
- l Con centration:
I
% Mortality:
10 l
Mean OffspringFemale:
l 31. C)
% Reduction from Control:
1.'%
g
I:'-
r 1.
I Environmental Testing Solutions, Inc.
Page 3 of 6 Species: Ceriodaphnia dubia
..:.':.CdNaCLCR #:
SurvIval andRdprodudidn'Daoa d;
800 mg NaCltL D~~~~~~~
e" - Repticate ntuber 3*
-'4 5
- ,6 1&
7 &
U..
N
- 1 1 0..
1 Youngproduced
+,
O ~~~~~~~~~~~
'0S'l~~-0)
'l C) tlrc
¢ I
Young produced 0-C )O~
Adult mortality 7
L...
1 2
Young produced p.
° o
Adult mortality 1
L tt_
3 Young produced C
C>
s 0
O P
O Adult mortality 1
U.
L U
L l
U.
U _
4 Young produced z
b Adult mortality U.
L 1
T ?
s Young produced IQ jJ I
0 k
1 jj l 02 Adult mortality L-U..
U.
L-L-
6 Young produced 0
9 O
O OJ 1
O O-Adultmortaity L
L L
- l.
L-lL L
7 Young produced
-Il-1-1 Izo 1
1i[IlL Io I 1' Final Adult Mortality I
?
I T
I t
1 1
Concentration:
% Mortality:
rft l Mean Offspring/Female:
a 63 l
% Reduction from Control:
(.A,7l 1000 mg NaCI/L Survival and Reproduction Data I_____
IRepUcate number Dal I
l 2
l 3
14 5
l 6
7 1 8
l 9
I 10 Il Young produced l0i0 iC:l(
O I 0 a I
T C° l o l l ~~Adult mortality l
>lL l
t-l Il l ll t
1 Young produced C)(
I 3 z YounC produced
)
C)
O lGL t
1
( ) IO IO I
l l ~~Adult mortality Ll-r L-l L lClell
~~~
~
~~~.
l 3
l Young produced 0
l l
~Adult mortility L-L-
L__lLli{
l 5 Young produced i
q1<
T6 I
to in to T
L 1231-Adult mortality LL-..
U l
L
=.
L L
l Young produced l
(
O
()l1 0l1 Adult mortality
[
U l
l I
I
]
U Young produced Q __
_ i 11i 1
i O l
lt Adult mortality l
l l__
__ll t_
l
- t.
1 7 l Eounproduced T h _.
Ulj U
IN.
z 1
e IIr rio Il 9
I F
naI A d u
l m tal it ly
_l ____
l_(___
l=
L
..l L l t iConcentration l% Mortality:C)'7
! Me n Offsprng/Female:
12(..(o
% Reduction from Control: 2047,
- i... -
~ 4
,;,.i.*
- 9..
Environmental Testing Solutions, Inc.
Page 4iaf
- CdNaCLCR #. :
.Survival andReproduction Data t
Species: Ceriodaphnia dubia I1 I..-
1200 mg NaC/L tv rReolica number ^*^t>.Ai
?'..
Day i
2 3
4 5
6 I 7-'
t 8
210-Young produced O
O
- o *. O 7Vf O '0
- ' ^
Adultmortaiity L ; r-L U
L L ai *L'-
2 Young produced p Q Q
Q C
Adult mortality 1%
L L..
t.
3 Young produced O
)
C C;L
(
O Adult mortality
.L
- L L
4 Young produced l
0
._ 3 Z
o 1 3 Adult mortality L
I u
S Young produced l
[
S cl
.1 I.
1 q
Adult morta[ity[
\\
v e
L 6
l Young produced C)
C)
CD C) c'C C) l I o C0 Adult mortayl IL k-
- l.
7 Young produced l o_
1 l
I 3
~~I.
-b I Totalyoungproduced l Iz ILI I
19 (4
9l Final Adult Mortality j
L j
j j
j Concentration:
% Mortalitv:
IMean Offspring/Female:
113-o
% Reduction from Control:
1 1400 mg NaCI/L Survival and Reproduction Data i i______________
Replicate number M
Dy 1
I T 2 7 3 1
4 1
5 1
6 7-S a
9 I
10 1
Il Young produced l O
1 t
)t O
l 1;
l O _ l O
Dy rAdult mortality L
I L
L L
2 j Young produced o
l Adult mortauIt L
l1 I
l1 3
Young produced C)
_)
Adult mortay l
t L,
I-1 L
L-L!
LI Yongproduced o
Z l
Z 01 C) 1 Adultmortalty L-LL l
1-1 L
-lIL s
Young produced C) 0 2
0 I
l sI Adult mortality l
L L
6 lYoung produced Adult mortalit I
7 1 Young produced j3 1
5 j 8 j
Ll lTotl y
oun nproduce 1:
_-t-lFtal Adult mortaity l
1 U
l__
Concentration:
A %Mortality:
I MOI 7 ~~ Young produced 3
5I~~~~1~~ rz ei Offprng-eal:
.(
% Reduction from Control:
o.Z%1
.. A W
(.. 5' V A7 I.
t.'..
i.
'i I.
i
Environmcntal Testing Solutions, Inc.
Verification of Ceriodapliia Reproduction Totals Control I)ay
=ie 1cate number otal 1
2 3
4 5
6 7
8 9
10 1
0 0
0 0
0 0
0 O
O 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
6 4
5 5
5 0
5 so 5
12 13 1 1 1 1 10 10 13 12 12 10 114 6
0 0
0 0
1 0
0 0
0 U
7 18 18 17 17 14 16 17 20 16 16 169 Tolal 35 37 32 34 29 31 35 37 33 31 334 600 mg NaCI/L DAY____
it nicate number Total Ihy 2li 3 l4 5
6 7
8 9
10 I
0 O
O O
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 4
4 6
6 5
5 5
4 5
5 5
50 5
12 13 12 I
12 13 10 10 10 13 116 6
0 00 0
0-0 0
0 0
0 0
V IS 16 18 917 19 19 15 16 17 174 Total 34 35 36 30 34 37 33 30 31 35 340 800 mg NaCI/L Day
-I Ricate number Total 1
2 i 3 1 4 S 1 6 7
8.1 9 1 10 1
0 0
0 o I o o
o o
o 0
2 0
0 0
0 0
0 0
0 0
0 0
3 0
0 0
0 0
0 0
0 0
0 0
4 4
6 5
5 4
5 6
5 5
4 49 5
10 13 It 10 10 14 11 10 10 12 III 6
0 0
0 0
0 0
0 0
0 0
0 7
17 17 20 17 17 16
- 18.
15 15 19 171 Total 31 361 361 321 31 351 35 30 30 35 33 1000 mg NaCUJL
, 4".
M.
I y iRelcate nwber Total DLy I31 3
4 5
6 7
8 9
10 I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 1.0 0
3 00 0
0 0 0
0 0
0 0
0 4
4 4
5 4
5 6
5 3
4 4
44 5
I I 0
1 0 1 0 1 0 1 3 10 12 S
.11 105 6
0 0
0 0
0 0
0 0
0 0
0 7
1 0 12 16 1 3 13 10 10 13 9 1 1 17 Totll 25 26 31 27 28 28 21 26 2
1200 mg NaCIIL Day R
rate number 1 21 3 4
5 6 17 8
9.
10 21 0
0 0
0 0
0 0
0 0
1 0
3 1 0 0
0 0
0 0
0 0
0.1.O 0
4 0
0 3
2 0
3 0
2 2 1 2 14 5
8 7
3 5
9 6
4 l7 6!
4 59 6
0 0
0 0
0 0
0 0
0°.
°.0 0
7 4
4 3
9 10 10 4
.10
--6'
'3 63 Total 12119 161919 8
1914.9 136 14 0 0 mg NaCIL Re Ucate number Day
~i
-~
.~~
Ttl 1~~~~~
1 61
,.1 O
I 0
0 0
0 0
0 0
0
..'0.
,O.
0 2l0 0
0 0
0 0
l O.
i O
I5rO.
3 0
01 0 0
0 0 1 0
.0. 1 0 '1to.
0-4 0
0 0
2 2
0 0
A0.
I0.
p3j t-,;7.
5 3
l 5
0 0
4 4 l
~ -Oz 5
- 6.
- 2S l
6 0
0 0
0 0
0 0 10; v2s1 2
7 3
5 30 4
2 7* 40
--O~ A~
K29~
.~~~~~~~~~~~M l Total l6 l6 2
13 r2 I
6~..
6
,4 F.
t 5-t.4.2ft.
Environmental Testing Solutions, Inc.
Chronic Whole EfFluent Toxicity Tcst (EPA-821-R-02-013, Method 1002.0)
Species:
eriodaphnia dubia Quality Control Verification of Data Entry, Calculations, anl Statistical Analyses I.
Test number Test dates:
CdNaCICR #44 October 7-14,2003 Revewed sqr/_
Concentration Replicate number Survival Aveage reproductloa
, Cddt F
r e m~~
de. (%)
ena l (14)
(mg/L NaCI) 1 2
3 4
S 6
7 8
9 10
(%)
(of psIusonae)
Control 35 37 32 34 29 31 35 37 33 31 100 33.4 e! ;o.
Not applicable 600 34 35 36 35 34 37 33 30 31 35 100 34.0
.., 64.j
-1.S 800 31 36 36 32 31 35 35 30 30 35 100 33.1
- 7.6 0.9 1000 25 26 31 27 28 29 25 28 21 26 100 266
.1&2s*
20.4 1200 12 11 9
16 19 19 a
19 14 9
too 13.6 to32.6 S9.3 1400 6
6 13 2
6 6
It 5
2 9
90 66 5
7
. 80.2 Dunnett's NISD value:
PMSD:
3.169 9.5 NtSD Minimum Significant Difference I'MSD -
Percent Minimum Significant Difference s-p'$'
PIMSD is a measure of test precision. The PMSD is the minimun percent difference between the confrol and tt st ean be dacl atatitcaly significant in a whole effluent toxicity test. On average, a significant difference occurs for Envirvnenial Teting Solliti, h& efhronic toxicity Wels s-hcn a toxicant reduces Ccriodaphnia reproduction by 9.9% from the control.
.^
i LowerPMSD bound determined by USEPA(10 percentile) =1%
'i* ;.
I 10/
Upper PNMSD bound determined by USEPA (9 0e percentile)- 37; r
The lower and upper bounds were calculated by the USEPA using 393 tests conducted 6fim 33 laboratorie for a ns iin chu i reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in tholc Efluent Toxicity Applications Under the Nationul Pollutant Discharge Elimination Program.
Agency, Cincinnati, Ol.
Environmental Testing Solutions, Inc.
Statistical Analyses Corl~ophuia SrwIl saw Rerduction Tsrt-Rlitbwet. '
S4wt 0.
to'0 7'
ID:
COLR a a US
^
REFltTot J
_ad Dsts 20114)03 Lab NA 3=*I Dow.
pruwC.e OIOnTC-(EAJ21-l02013)
T-3ped-CD-Codapos~ dubis Coeu-OfL 1
2 3
4 S
7 8
- -9*
D.Ci7ntl SS.O 37.000 32.000 34.C00 29.000) 3.000.
, 35.000 v.37.D00
.s 33.000 (31.000C V, '
600 3.000 33.000 36000
.33,000
- 34.000
-. 37.000 I33000
'-30000 3000 S000 000 ~
- ~*3 000
-V
- W 3100 X
34.00 sdo: '
35.00 0
0000 Y36.00000'
- < *t,*E low 203.00 21.000 2
7.0o 2.o7
- 29 000 7
O V
1200 12.000 100o o9.000 16.000 1900 190
- R ts0xo Ii-' *
?
- 40 &OWc tWOC 1000 soo
.0007 ooo
-r,
'Ie ae
~
t; Tfa"t:w Ut aFrtel I.......
-TaU"4r Caw~fL Moan I-Moaa Mean Min Mal CVY N
t 5t at Critical M5D meS r4.Mea D t 40 7
340
.0000 3.400 29.000 374000 1.000 1L0 3.700 00'0 600 3400 1o.01S0 34.000 30.000 7.000 6.3sX Io4 1
.433 2.2SSJ
.369
- 33.7070 1100oo soo 3110 0o.99to 3.lo00 30.0007 36.100 7.S96 to (L216 2.2117 3.169 MAN0<
0.96ZZ 10 26.600 0.7964 26.600 21.000 31.000 10.211 10 4.907 2B7 3.169 26.600 0.7s3
'1200 31.600 0.402 13.600 MOO 19.000 315.j 1
14.2SS z.2s 3,169 1.600 0.4036
'IJOO 6.600 0.A976 6.600 L.0o 13.000 3.45 to 19.339 2.2B7
.l169 6.600 0.1958 Asatlr iTs s
Ststicl Crit<^l 5k Kurt Kohnoilorov D Test incate noroW datnbueon p > 001) 0499B43666 1L035 0.06606336 0.5946375 Btlntes Test rlricte equal wrinces p - 0.291 6.21 52174 1 5.08431l71 Hypoth.le Test(t-taltO 0.57 NOE:C tOEC ChV TU MS041 NM5D M31 M5E r Pt h dt Duuiets Test soo IODO 894.42n191 3.16M3852 0.094S7437 U374.07 9.60185185 7.1E-30 5.54 Trfemints vs D-Controt Unear Iat iat-an (200 Rssoples Cselt MdaL S
M SY CL Skeu Coa 8I 33.413 39.2306661 717.402664 85.09465 2.7820 c
00 s s.230769 20.1320687 4.0248208 916.389376
-0.332 I*S 937.02693 21.18072 sco.ss26 20 0
0
.27.
0.2403
.6 o
0 6.923077 20.1158609 64s.31609 1016.6902 1n3071 0.6 Kcl r
1020.3S462 i
ZS 14456 993.793282 10424064 00.4606 IC40 0l.t33ll 3.658o t1073t11747 1L20.381 0.064 M
a IC5S 8
050 91642.6618 0943 3.74639 96 1874..662i 0.306
.57 02 6
0 0.. e
-0.1~~~~~~~~~~~~~~~~~
DC40 209 5 1.658809 1073 27747 1220.73381l0.0 ICSO 2150 263266288 121946398 128426622 0.3062 0.7Doz milil DoseRWI90300la 40 35 1-tal. 0.05 level of significance 25,
.20 0.
a.
10~
10-14-03 i
I-II.'
I I
Environmental Testing Solutions, Inc.
~Page 5 of 6.'
i
- j.
Species: Ceriodaphnia dubia Daily Chemistry:'-
I
, I
- -~
- j ~
{-
f-I, I
-';Y Ri:L.
Analyst ~
Concentration Parameter
_~~~I pH (S.U.)
DO (m/L)
, _~~I.
CONTROL Conductivity (wmnhoslcm)
I Alkalinity (mg CaCOlL) 5 _ _ _
Hardness (mg CaCO-lL)
I t
Temperature (CC)
I I
I I
I I
pH (S.U.)
A n___
4._
DO (mglL) P, - >Z1(
ZH(
600 mg NaCIL Conductivity 110 Temperature I
I
(° C)
_______trl~
y pH (S.U.)
S S
DO (mg/L)
A3 J
~
800 mg NaC/L Conductivity (jimhos/cm)
I B 5_
Temperature 2
(°C) 2t____
ZS, 2
_.1 ILA
__Z____
pH (S.U.)
' q._
DO (mfL)
.33
___i_
1000 mg NaCllL Conductivity (pimhos/cm) 2250 d__
l2o Tcmperaturc z4.
2
.5.
Z.
~
~~~~~
pH (S. U.)
,5 48 DO (mefL)
(3 4 Z
__8 1200 mg NaClL Conductivity 239l
(,unhos/cm)
Z t
0 Temperature I
s 4q IS
'(C) 7_. V ZS.O
__I_____
pH1(S.U.)
q9
.s qf w-3
.a DO (mzfL) le.
8-S
- l.
8 Z 1400 mg NaCI/L afHOt Z-LQ Temperature
(°C)
_4___
ZS_
25__.
Z.
STOCK Conductivity L_________(pmhos/cm) 130000 won"_
I I
I I
Initial Final Initial Final Initial Final I
a a
I -
S
Environmental Testing Solutions, Inc.
ie.
Species: Codaphnia dbia Page 6 of 6 1. -
CdNaCLCR#
.
- '. - iv..
- I --.:
, -;', _. _- '. 1-1 AJ ' --
'4-:-
-- '..I e
a ;. -i\\-t
- Analyst Concentration Parameter -.--
R CONTRlOL oH (S.U.)
5 _
DO (mgL)
Conductivity (wmhos1cm)
Alkalinity (mg CaCOJL) xcI Hardness (mg CaCO,/L)_
Temperature
(°C)
I I
.9I u
11 DH (S.U.)
I I
600 mg NaCI/L
.~~
~ -
DO (mg/L) 1.9
- 8.
e o 1
a-.q (Omhos/cm)j 135b woo l,=ii.
I Imco m Temperature (IC) zq6&
zq.k ZS-I pH (S.U.)
i eo 4
1 DO (mg/L)
E3 8.1 1
6W
_O 800 mg NaCUL Conductivity PZ0 I-;eo
~~~~(jpmhos/cm) 111 I0S 11(oo Temperature
~
z.,
- z.
v-/
z ZI 2
(°C)
__Z I_
1.75c.:4 2s l
pH(S.U.)
qns1
-*. ie
.e_3 DO (m/gL) 09.'I
- 81.
1 f3_ 0 1000 mgNa/LCnutvy 1000 mgNaCI/L(gmhdostcm)t Zl5D e
15 w
Z1 eo z 130 Temperaturc (QC)
_ s____)
Z__
_4 Z_
Z_._5 pH (S.U.)
.53 1.53
- 5.
.6 DO (mg/L).
D
- 3. l l___
- 1200 mgNaCIL Conductivity 1 mg NaCV (gmhoslcm)
Z55o Z5eP 25e Z
.F_
Temperature I
Z Z4. 5 (C
)
nH (S.U.1
_k. ?3 302Z 1 AD I
1400 mg NaCI/L STOCK DO (mg/L) 4.0 A1.
- 8.
1 4
1- -19 Conductivity 2q10 2 q O
?lqo (imhos/cm)
Z________
I I a
Temperature (or'
.I L 0 2J'1q.L i % -1I Conductivity (umhos/cm)
_$MM 1
_--I Z5.l 4...,I=
= r-Initial Final Initial Final Initial Final Initial Final I.
.9 a
-
PERMITTEE NAMEIADDRESS (Include FacititvNamel ocation ifDifferenO Name TVA SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ____________
_(IN IER OFIC6E S B-SODDY -DAISY TN 37384 Facit h__VA-SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT 1OMR) 0MB No. 20400004 (SUBR 01)
MNo20004
[
TNOO26450 103 G l F - FINAL I
i DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND I
i ING ERIO I
EFFLUENT I EA IMI A
YEAR O DA From 031 9j 1 TO 0310 j4 31~
- -- NO DISCHARGE C
NOTE: Read Instructions before completing this form.
ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREUENCY SAMPLE EX OF TYPE N.
AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.0 8.6 12 0
14/31 GRAB MEASUREMENT 00400 1
0 0
.,PERMIT.' -
6.0
+-
. 9.0.
SU THREE/
GRAB EFFLUENT GROSS VALUE REURMET-MINIMUM MAXIMUM WEEK.
SOLIDS, TOTAL SUSPENDED, SAMPLE 158 169 26 13 14 19 0
5/31 GRAB MEASUREMENT 00530 1
0 0
RPERMIT;,
380 1250 W
LBSEDY
.30 MG/
EEKLY GRAB EFFLUENT GOSS VALUEREQUIREMENT EFFLUENT GROSS VALUE T
MO AVG DAILY MX MO AVG DAILY MX OIL AND GREASE SAMPLE
<60
<64 26
<5
<5 19 0
5/31 GRAB MEASUREMENT 00556 1
0 0
PERMIT-190 2
S 20 M
WEEKLY GRAB EFFLUENT GROSS VALUE RE.,IREMENT.MO AVG-DAILY MX
-MOAVG-DAILY MX...
FLOW, IN CONDUIT OR THRU SAMPLE 1.327 1.524 03 0
31 /31 TOTAL TREATMENT PLANT MEASUREMENT 50050 1
0 0
-.. PERMIT ~-,_
REPORT--- --.1REPORT' MGD
- .*.DAILY
- TOTAL, REQUIREMENT-EFFLUENT GROSS VALUE
- MO AVG D
MAY X
M.
SAMPLE MEASUREMENT
- PERMI.-.
REQUIREMENT SAMPLE MEASUREMENT
- -;PERMIT-.
.,,.1/4'..,_;..,.,..,
REQUIREMENT; j."~K SAMPLE MEASUREMENT PERMIT. -,
REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify tnder penalty of law that this document and all attachrents were prepared under my r
f\\
t TELEPHONE DATE directron or supervision in accordance with a System designed to assure that qualified personnel
.4uI 4AC)
Richard T. Purcell property gather and evaluate the informrration submitted. Based on my quiryof the person or n~~~~~~~prsronh mne the ystem. ore tse persotns tdirectly responsible for gathering thle Acting Environmental Supervisor 423 843-6700 03 1 1 1 3 Site Vice President information, thte Inormation submitted is. to Ite best of my knowledgearnd belief. true.
Site Vice President accurale, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED______OR___PRINTED_______
ntormation. incuding the possibility of fine and impnsonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED COOEANYAOL__________________________
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EP For 330_ _RE _l9 Prviu edtin may be _se
___.g
_ _T 1 __
EPA Form 3320-1 (REV 399)
Previous editions may be used Page 1 o 1
PERMITTEE NAMEIADDRESS Include Facility Namelocation ifOifferentJ Name TVA - SEQUOYAH NUCLEAR PLANT Address P._OBOX 2000 ____________
_QffflROM(CE ao -2 SODDY -DAISY_
TN 37384 Faciltv_ VA-SEOUOYAH NUCLEARPLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040.0004 l
TNOO26450
[
107 G l F-FINAL PERMIT NUMBER l l DISCHARGE NUMBER METAL CLEANING WASTE POND I
MONITORING ERIOP IYEAR MO DAY IYEAR MO AY From 03 10 I01 To 03 1 10 31 EFFLUENT
- -- NO DISCHARGE
=
NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENC SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 8.4 8.5 12 0
2/31 GRAB MEASUREMENT 00400 1
0 0
PERMIT...~.~~ee*.
.- ee~e
- 6. 0 ~:.
- o~
9.0.
SU
.DAILY
- GRAB-EFFLUENT GROSS VALUE REMUIREMENT e -
.0 PHOSPHORUS, TOTAL (AS P)
SAMPLE 19 MEASUREMENT 065 1
0 0
':PERMIT
~
- ,~..~**e'10~.MIGIL I,.DAILY,, COMPOS
'REQUIREMENT.
EFFLUENT GROSS VALUE DAILY M_
SOLIDS, TOTAL SUSPENDED SAMPLE e******
10 19 0
2/31 COMPOS MEASUREMENT 00530 1
0 0
PERMIT..
M{.
v
/
'3GL
- DAILY, COMPOS EFFLUENT GROSS VALUEREURMN DIYM 7
OIL AND GREASE SAMPLE
<5 19 0
2/31 GRAB MEASUREMENT 00556 1
0 0
- PERMT'V
!*j*;ee.~,;~
,ee**'~.'
.*t~~$
1 MGIL DAL GRAB EFFLUENT GROSS VALUE R
EQUIREMEN AL Y.
- i.
X
~DAILYAMK COPPER, TOTAL (AS CU)
SAMPLE
<0.010 19 0
2/31 COMPOS MEASUREMENT 01042 1
0 0
PERMIT I*.-
' ' i ~
MG[
Am, f.L DAILY., COMPOS REQUIREMEN L:.i.
EFFLUENT GROSS VALUE DAILY MX,__
SAMPLE 0.13 19 0
2/31 COMPOS MEASUREMENT 1045 1
0 0
~~~PERMIT...
DAILY*
1.0MPMGI 01045 1
0 0
REQUIREMENT M
L D
COMPOS EFFLUENT GRODSS VALUE, DALM____
FLOW, IN CONDUIT OR THRU SAMPLE 0.044 0.045 03 0
231 CAICTO TREATMENT PLANT MEASUREMENT 50050 1
0 0
'PERMIT
'EOT REPORT MG D*******AL C. Ai. C EFFLUENT GROSS VALUE RE.UIREMENT',
MOAILYDILYMXCAL NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penatly of law that this document and all attachments were prepared under my I
TELEPHONE DATE irection or supervision in accordance with a system designed to assure that qualified personnel LELElONEflf AA Richard T. Purcell properly gather and evaluate the Information submitted 8ased on my Inquiryof the person or A.'l9 idw-persons who mnage te system. or those persons directy responsible for gathering the Aor 423 843.6700 03 11 13 information, the information submitted is, o the best of my knwedge and belief true I
Site Vice President accurate. and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE T IDormaDion, inDuding MO possibility of fine end imprisonment for tinowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE l COMMENTS AND EXPLANATION OF ANY ViOLATIONS (Reference all attachments here)
No phosphorous bearing cleaning solutions were used. Metal pond was released October 30-31, 2003.
EP Fo_
332.1 (RE 3_9 r~u.dtn.ab ue Pa, 1.. of 1 EPA Form 3320.1 (REV 3199)
Previous editions maybe used Page of
PERMITTEE NAME/ADDRESS (nclude FacilityNameLocation if Different)
Name _
VA-SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 INLTEROFFII.E LB SODDY - DAISY TN 37384 LaciW WA -.SEOUOYAH NUCLEAR PLAN T Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES)
MAJOR DISCHARGE MONITORING REPORT (MRJ (SUBR 01)
TNO6450 110 G l F - FINAL I
PERMIT NUMBER I DISCHARGE NUMBER RECYCLED COOLING WATER Form Approved.
OMB No. 2040-0004 I
MOiNtITORING-iwpFRI I
EFFLUENT
~~1*~~~'~ ~
I YER M
DAY YEA MO AX4 From Lo03 1I 01Io1 3 ToL jA 31 NO DISCHARGE 3
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 00400 1
0 0
- ~.'*e
.*eee
~:-.,.>;'9.0 S u DAILY
'GRAB EFFLUENT GROSS VALUE IMUM 2M.UM7:
SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 0530 1
0 0
PERMIT' MG/
DAILY COMPOS
- REQUIREMENT 1
EFFLUENT GROSS VALUE DAILM
,VIX OIL AND GREASE SAMPLE MEASUREMENT 19 00556 1
0 0
a PERMIT.>;
...... **ei**** >
1**1*1.
' 1 MG/L
-DAILY COMPOS EFFLUENT GROSS VALUE REQUIREMENT.
DAL. M-
"'e' 8
:_Ai CROPPE TOTAL (AS CU)
SAMPLE 19***
MEASUREMENT 1
EFFLUENT GROSS VALUE EMENT A
- RONPR, TOTAL (AS E
)
SAMPLE 19 MEASUREMENT 19 0104 1
0 0
PERMIT; t
'01 MG DAILY COMPOS EFFLUENT GROSS VALUE
~
REQUIREMENT*.
ALM'.~
PHOS, HORAUTLO (ASRU)
SAMPLE 19 MEASUREMENT 1
01065 1
0 0
PERMIT';
~**
REQUIREMENTM/
DAI__
LY EFFLUENT GROSS VALUE FLOWPNHONUTOR THRUP SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1
0 0
,PERMIT
- REPRT, O
MGD DAILY CALCTD REQUIREMNB
.T-"
EFFLUENT GROSS VALUE OV DIY X NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of law that this document and all attachmenls were prepared under my l
TELEPHONE DATE frion or supervision in accordance with a system designed to assure that qualired personnel
- I1ikL,
(
n
,Y^.rP Richard T. Purcell property gather and evaluate the information subminted. Based on my inquiry of the persnonor t
i v
persons who manae this system ord thos5§ peronsdtespyonsiefo atein t~e Acting9 Environrrtental Supervsor 423 8436700 03 1 1 13 Site Vice President infoatin. the nformaittion submitted is.* to the best of my krnoledge and belief, true, S ite Vice President acurale, and complete. I am aware that there are significant penalties tor submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, Incluing the possibility of fne and Imipnsonent for kuowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EP For 3320.1 (RE 3_
Prevou e.tin mab use Page.1 of 1 EPA Form 3320-1 (REV 399)
Previous editions may be used Page of
PERMITTEE NAME/ADDRESS (Include Facility Name/tocation ifDifferent)
Name TVA -SEQUOYAH NUCLEAR PLANT Addess P.O. B X 000
__ NTERO E B-SODDY DAISY TN 37384 factjj_ TVA. SEQUOYAH NUC LEA PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT DMR)
(SUBR01)
I TN0065 1
116 G F - FINAL I
PERMIT NUMBER I DISCHARGE NUMBER BACKWASH Form Approved.
OMB No. 2040-0004 From 03 lo. I0I Tol 03 1J10 1.
EFFLUENT
... NO DISCHARGE
=
NOTF Read instnirtions before cormnfetina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE
~~~
~~~
~ ~~EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE 0**
- 0 9A 0
1 / 31 VISUAL MEASUREMENT 01345 1
~~~
0 2~~ PERMI~T ~'
-REPORT:4.
PASS=O SEE--..VISUAL EFFLUENT GROSS VALUE EN'MO
- TOTAL, FAIL PERMIT OIL AND GREASE VISUAL SAMPLE 0
94 1 /31 VISUAL MEASUREMENT 84066 1
0 0
RPERMIT;.
.cRE NO0T,'i'SEE VISUAL-RESSUREMENT_
EFFLUENT GROSS VALUE REQUIREMENT
- R EP O R ';
Y ES r '*
PE MI
___=
~~~~~~~MO TOTAL PRI SAMPLE MEASUREMENT
'.PERMIT2;,!
REQUIREMENT.
.-. SURPLMN SAMPLE MEASUREMENT PERMIT -
REQUIREMENT' SAMPLE MEASUREMENT PERMIT.;
RE'UIREMENt'
. i.
SAMPLE MEASUREMENT REQUIREMENT' SAMPLE MEASUREMENT
'PERMIT REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Celify under penalty of law that this document and all atladiments were prepared under my TELEPHONE DATE direction or supervision i accordance with a system designed lo asswe that qualified personnel l
,fl/
atJ.
I Richard T. Purcell property gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system or ftose persons directly responsible for gatherig the Acting Environmental Supervisor 423 843-6700 03 11 13 Site Vice President information, the inormation submitted is. to t best of my knowledge end belief.
SINTUEOFPICIAeEEUT.
TYPED OR PRINTED inoation. ncluding the possibility of rne and Imrnsonrent for knowiug violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OFANYVIOLATIONS Reterence aattachmentshere)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EP _or 3320. (RE 3_
Preiou edtin mab use Pag 1..
of 1 EPA Form 3320.1 (REV 3199)
Previous editions may be used Page f
PERMITTEE NAMEIADDRESS (Include Facility NamelLocation if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O B 2000
_LOLTEROM(E.Sl0 -
SODDY-DAISY TN 37384 Faily _TVA SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (PDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TNO026450 117 G F - FINAL I
PERMIT NUMBER lDISCHARGE NUMBER BACKWASH I
M IiIONITOR NtIG_,pEERIOI i
EFFLUENT Form Approved.
OMB No. 2040-0004 YEAR IMO I
DAY YEAR O
DAY From 03 1 0 01 To[ 03 _I10 31j
- -- NO DISCHARGE z
NnT: Read instructions bWfore comoletina this form.
ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREOUENCY SAMPLE
~EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE 1_
9A 0
1/31 VISUAL MEASUREMENT 01345 1
0 0
1-PERMIT:.>.
.r-
~
- REPORT, PASS=O SEE VISUAL EFFLUENT GROSS VALUE REQUREMEt>...
S
- .)
MO FAIL PERMIT OIL AND GREASE VISUAL SAMPLE 1131 VISUAL MEASUREMENT 94 84066 1
0 0
..PERMIT-,
.' 7RPR YES=1
.K**,*
- Ah.'\\
- I**
S.
VISUAL EFFLUENT ROSS VALU REQUIREMENT~,
0t.
.MOs-TOA
(,~
2.F.
0__
PEM_
SAMPLE MEASUREMENT REQUIREMENT G. V.A SAMPLE MEASUREMENT REQUIREMENT' SAMPLE MEASUREMENT PERMIT..
.i REQUIREMENT.
SAMPLE MEASUREMENT REQUIREMENT
- ~..*
SAMPLE MEASUREMENT REQUIREMENT j)
~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE irection or supervision in accordance with a system designed to assure that qualifed personnel Richard T. Purcell property gather and evaluatthe information submitted. Basedonmy inquiryof the person or persons who manage the system or those persons directly responsible for gatherg te ActingEnvironmental Supervisor 423 843-6700 03 11 13 Site Vice President
,rinformat ion, the information submitted is.* to the best of my koee and belief, SINTUEOFPINIALEECTV Site \\fice President r
curate, and complete. I am aware that there are significant penalties for submiting false OF PRINCIPAL EXECUTIVE TYPED______OR___PRINTED_____
information. including the possibility of fine and Imipsonment for knowing violations.
OFFICER OR AUTHORIZED AGENT ARtEA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EPA~.
For 32.1 (REV 3199)
Previous edition..ay be.used Aage_1 Of EPA Form 3320-i (REV 3199)
Previous editions may be used Page 1 of 1
PERMITrEE NAME/ADDRESS (Include FacitityNameltocaton iDifferent)
Name TVA - SEQUOYAH NUCLEAR PLANT____
Address P.O. BOX 2000
_ _ _ LNTEROFfE S B)_.
SODDY -DAISY TN 37384 Facility_ TvA SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES)
MAJOR I
DISCHARGE MONITORING REPORT (DMR)
(SUBR t)
I TN0026450 118 G F - FINAL PERMIT NUMBER DISCHARGE NUMBER WASTEWATER & STORM WATER I
-MONITC GQ ERIOD I j EFFLUENT I YEAR MO DAY I YEAR MO K1A
- -*Cr fle'-UAmf'M F1...
Form Approved.
OMB No. 2040-0004 ATTN: Stephanie A. Howard Froml 03 10 01 I To 03 10 31 NOTE: Read Instructions before completinq this form.
NO.
FREOUENCYI SAMPLE EX OFYIS OF TYPE IANALYSI UNITS 19 00300 1
0 0
EFFLUENT GROSS VALUE
' PERMIT REQUIREMENT iI i
- ^,
e n I--...
- i*****
I-,., ******** -..
I
,-l -;.v.;. ;
2.0 DAIY N I..
I DAILY MN I-'
I". ;.
MGI TWiCEI. GRAB WEEK I.
SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 19 EFFLUENT GROSS VALUE PE T
- l.
.X WEEK' SOLIDS, SETTLEABLE SAMPLE 2*
MEASUREMENT 25 00545 1
0 0
PERM IT-',"'.e e c..
- a.
- * * ** e.
- - -~a a e ;
10 '
L/
-O NCE!
RA EFFLUENT GROSS VALUE AIERME
- T LY MX LMONTH-G FLOW, IN CONDUIT OR THRU SAMPLE
- e******
TREATMENT PLANT MEASUREMENT 03 50050 1
0 0
PERMITN REPORT. ' -
REPORT MGD
- ^****.
^
e
.. e***
O ESIM MEASUREMETT OC/ETM EFFLUENT GROSS VALUE REUI R EMT BATCH SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT PERMIT-REQUIREMENT SAMPLE MEASUREMENT
.PE.RM1T-REQUIREMENT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all atachments were prepared under my I
I I
TELEPHONE DATE Richard T. Purcell direction Of suPersion In accordance with a system designed to assure that qualified personnel TEEP N
DLATE Richard T.
Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage te systen or those persons directly responsible for gathering theActing Environmental Supervisor Site Vice President information, the Information submitted is to the best of my knowledge and belief tnue.
- ecaxale, 423 843-6700 03 1
c I
1 13 Site Vice President ea curate. and complete I am awvare that there are signifrcant penatties for submitting false SIGNATURE OF PRINCIPAL EXECUTI^VE i
TYPED_________________
OR P Tinrormation, including the possibility of line and imprisonment for knowing viotations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TY PED O R PR IN TED
_______ J CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EP A Fo..30
(..
1 PrA u edtin ma. u.e Pag.. of 1 EPA FOrM 3320-1 REV 31991 Previous editions ntay be used Page I of 1