ML032670626
| ML032670626 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 09/10/2003 |
| From: | Howard S Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML032670626 (113) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 September 10, 2003 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6e Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR AUGUST 2003 Enclosed is the August 2003 Discharge Monitoring Report for Sequoyah Nuclear Plant Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Stephanie A. Howard Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
- U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Afled an Od W
PERMITTEE NAME/ADDRESS (Inctude Facility NameAocaton fDiffement)
Namne TVA - SEQUOYAH NUCLEAR PLANT Address P0O BOX 2000
__jre~ _ {NE.F<O)9E B20M
_fLE0flE
SODDY - DAISY TN 37384 Fad:ily WA. -SEQUOYAHNU CLEA PLAN T_
Location _AMILTON CQUMJY -
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
KWOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN 0026450 101 G I F-FINAL 11 1M+/-UER I
l DISCHARGE NUMBER I DIFFUSER DISCHARGE
-M-o-t-TORINrl PERIOD I EFFLUENT Form Approved.
OMB No. 2040-0004 Fro m 0308I 1
To 03 1 08 1 1 NO DISCHARGE El]-
NnTF-Read Instructions before completina this form.
ATTN: Stephanie A. Howard PARAMETER~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~NT ORa LOADINGeoe opl~g hs rn PARAMETER
\\
i QUANTIY OR LOADING QUALITY OR CONCENTRATION NO. IfRtEOUENCY SAMPLE
_ _ _ _ _ _EX O F TY PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS LYSIS TEMPERATURE, WATER DEG.
SAMPLE 29.2 04 0
31 31 MODELD CENTIGRADE MEASUREMENT2920 0010 Z
0 0
7 DEG.i*
C. 7e 7ia~.~
INSTREAM MONITORING RTDEG.
C.
R TEMPERATURE, WATER DEG.
SAMPLE 29~0 3/1 ROD CENTIGRADE MEASUREMENT 42.9 04 O
31 31 RCORDR 00010 1
0 0
>,,EM.7~~f2
=.T7; V7'~=*1.KŽ**'*K-REORJ7 DEG. C.
~
~
iQ EFFLUENT GROSS VALUE DAIEG.
PH MEAMPLEME N
T ****t****
- 7.2 7.4 12 0
8/31 GRAB 00400 1
0 0
6E, 0 7"Wi****,
7
~oj
~
~
790' Su EFFLUENT GROSS VALUE S
U M
M SOLIDS, TOTAL SUSPENDED SAMPLE 6
6 19 0
4/31 GRB MEASUREMENT 661
/1 GA 0530 1
0 i, I 0 i
VI!~
- a~~,
- ~.1T*e*'3 MGJ LKL EFFLUENT GROSS VALUE M
GILYM r
OIL AND GREASE SAMPLE c
c5 19 O
4/31 GRAB MEASUREMENT 51 11 GA 00556 1
0 0
~-t*~~~'~1 K27 GL ERN GA EFFLUENT GROSS VALUE
._MO AVG DAIL MI FLOW, IN CONDUIT OR THRU SAMPLE 1589 03 0
31 /31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1
0 0 7" TR-EPW C.b R I
22 EFFLUENT GROSS VALUE GDA U
CHLORINE, TOTAL RESIDUAL SAMPLE
- 0.010 0.020 19 0
38/31 GRAB MEASUREMENT 50060 1
0 0PRI~7
~***A~'
3 5
MGJL WEKFEi9:
EFFLUENT GROSS VALUE MA)
NAMEmTITLE PRINCIPAL EXECUTIVE OFFICER I Cey under penelty d law thet this doaxler( mid all a*ladwrents wns prpared under my I t TELEPHONE DATE Erectin or supevislon hi e0rde wth s ystem designe to ass tm qulfid p Richard T. Purcell prope l" aodher eid evafue f krorfftedon submitted Baeow n oy Wqury of th pesno7*
awtho eninsg fth systm,% or those persons directly responsible for gathering; "
cigEvrnetlSpr~o 2
4-70 0
9 1
Site Vice President.
~
Iithe kIfoniation vt&mfttd Is, to the best of myW Waoedge arid baerle. true, 42I4-70 0
9 1
Sfte Vice President
- a.
and conqlets. I am awame that tr wer signiflcent penales for srbmitting fable SIGNATURE OF PRINCIPAL EXECUTIVE ORfonnation, InPdudhg th possibililty of fine and hnisonnwt b IboA v OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
________________________________________CODE COMMENTS AND EXPLANATION OF ANY ViOLATIONS (Reference naff achments here),
No closed mode operation. The following Injections occured: 1. PCL-222 (max. calc. conc. was 0.064mg/L-lmt 0.100mg/L) 2. CL-363 (max. caic. conc. was 0.OO9mg/L-Ilmft 0.100mg/L) 3. PCL-222/PCL-401 (max. calc. conc. was 0.022mg/L-limft 0.100mg/L) 4. H-1 30M (max. calc. conc. was 0.025mg/Le-lmit 0.050mg/L) 5. H-1 30M (low detection level analytical method was 0.047mg/-limrit 0.050mg/L))
EPA Form 33201 (REV 3/99) revious editons may be usd Page 1 01 EPA Fonn 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2
PERMITTEE NAME/ADDRESS (Incude FecilltvNameAocetlon ffDerent)
Name _
TVA - SEQUOYAH NUCLEAR PLANT dqesjP.BX
___2000
_ _ (lINTROfflQE%
S
_jODDtY Y-.AISY_ T 37384 fadvy A - EQUQAH NUCLEAR P TLA__
LocaIon _hAMILN COUNTY ATTN: Stephanie A. Howard NATIONAL POLLWANT DISCHARGE ELIMINATION SYSTEM (PDES)
MAJOR DISCHARGE MONITORING REPORT DRU M
(SUBR 01)
I TNO026450 101 G F - FINAL PERMIT NUMBER DI NME DIFFUSER DISCHARGE I
MONITARIn PFRInn I
EFFLUENT Form Approved.
OMB No. 2040-0004 rom YEAR I MO I DAY To MO I DAYTo From 03 l08 l01_ To03 1 08 l 31 1 NO DISCHARGE FI NOTE: Read Instructionse before com'pleting this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE
~~EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYST TEMPERATURE - C, RATE OF SAMPLE 0.2 6
31131 CALCTD CHANGE MEASUREMENT
- 0.
62 82234 1
0 0
MI DEG**7
- T
~~**~;
f
-C flNTINcLGT EFFLUENT GROSS VALUE E
DEG
- UOUS TEMP. DIFF. BETWEEN SAMPi &
SAMPLE 1.8 04 0
31/31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1
S 0
7M aee*:
~~
DEG. C.
~.QTN CLT EFFLUENT GROSS VALUE EG.C, 4
U BORON, TOTAL SAMPLE
<0.2
<0.2
<0.2 19 1 /31 GRAB MEASUREMENT 01022 1
0 0
MG/L ER'Ir
. NC EFFLUENT GROSS VALUE U
T
- R T
R MGIL O N r
SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT
.-- a E
4 SAMPLE MEASUREMENT NAMErTITLE PRINCIPAL EXECUTIVE OFFICER i C rt penaly of law thAs ths docmtanaltadntere prepred under my A
TELEPHONE DATE
_.redlOfi orserVisIon i accordanCe With a systemig dned to asste thad qualified personnel I I L
/I A
Richard T. Purcell gWte an evou* te Inormsiaon skmlatet Based an my kuxy of W person or WA SiteoV Om idft_
allm msteeIs.onsthetberestofnW bleorgalhedNgle M
Acting Environmental Supervisor 423 843-6700 03 09 10 Site Vice President e
4our mpe, xd eW I wn awae ta thr we sIgnca, penalties for submirlinga SIGNATURE OF PRINCIPAL EXECUTIVE I
TE RormaINon, including the posUlfty ofien end i rt fo k vi OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I
I__
CODEI COMMENTS AND EXPLANATiON OF ANY VIOLATIONS (Relerence eD atwachments here)
CCW data for August 2003 is attached.
EPA Form 3320-1 (REV 3199)
Previous edition$ may be used Page 2 of 2
CCW CHANNEL Datefrme Collected Extractable Petroleum Hydrocarbons Analysis DatelTime Analyst Method 08/04/2003 1010
<0.5 mgIL 08/08/2003 2110 JBR EPA 8015B 08/29/2003 0710
<0.5 mg/L 09/0312003 1118 JBR EPA 8015B CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 08/04/2003 1000
<0.5 mg/L 08/08/2003 2148 JBR EPA 8015B 08/29/2003 0715 1.3 mg/L 09103/2003 1156 JBR EPA8015B
PERMITTEE NAME/AODRESS (Indude Facl/tv NameaocatIon ffD/fernt)
Name TVA - SEQUOYAH NUCLEAR PLANT Address P_.OQQr
-O-
___fl1IfE~ROFFICE S? -2S -
_O DDY -kAISY_ jN 37384
_ y_DLA -SEf0QUOAH NCEA PLAN_
ocaon _AMILTON CQNTY ATTN: Stephanie A. Howard NATIONAL POLLUTAflT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMRJ (SUBR01)
OMB No. 2040-0004 I
TN0026450 I101 T
F - FINAL I
PERMIT NUMBER _
I DISCHARGE NUMBER]
BIOMONITORING FOR OUTFALL 101 I
MONITORINGk PERIODl I EFFLUENT From
- I YEAR o
MO IjDAYI From V03 l 08 I 01 ITo 03 1 08 1 31 NO DISCHARGE m
NOTE: Read Instructions before con i
.1 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certfy under pwat of low At thes docunert ed ill attecunent we prepared uwder my
_ arecion or supervision hI soooesnce with a systan desigd to asure, tt ualified personnel Richard T. Purcell properly gather and uste do ifmation sbmitted, Based on my hequiry of fe person or persons who mage mt sstem, or the persons directly responsible for gathering the I.nfnnaton, ft InO fom submitted Is, to Vw be of my knowledge and belief, true.
Site Vce President s,
rand a.Iemre lIhIt ttmm re e slwnmcant penalties for stbmiting fase
_______________________________ hdirmation, includirg th possibily of fine d knipuisonmnr for kwing violations.
TYPED OR PRINTED r
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all/atachments here)
Toxicity was sampled from August 3-8, 2003. Report Is attached.
EP For 330-(REV.......
./9 Pviu ed/tlons...
may.
be use Pag 1 of _
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of I
September 8, 2003 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, H130M SPECIAL STUDY, AUGUST, 2003 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected August 3-8 showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were >
100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN H130M-August 2003m
.5.
As TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: September 8. 2003
- 1.
Facility / Discharger:
Seguovah Nuclear Plant / TVA
- 2.
County / State:
Hamilton / Tennessee
- 3.
NPDES Permit #:
TN 0026450
- 4.
Type of Facility:
Nuclear-Fueled Electric Generating Plant
- 5.
Design Flow (MGD):
3.266
- 6.
Receiving Stream:
- 7.
1Q10:
2.992.4
- 8.
Outfall Tested:
101
- 9.
Dates Sampled:
August 3-8. 2003
- 10.
Average Flow on Days Sampled (MGD):
1570.5. 1579.0. 1578.5
- 11.
Pertinent Site Conditions:
HI30M was being injected from August 5-7, 2003. The dates and times for the injection of Hl3OM are provided below. The resulting H130M concentrations are summarized in Appendix B, along with other chemicals in use during sampling.
Injection Location Date/Start Time Date/Ending Time Injection Location (EDT)
(EDT)
Essential Raw Cooling Water (ERCW)-
Auut520315 Agst620/04 A Train Augut___2003100 Augut_6_2003100 Raw Cooling Water (RCW)
August 6, 2003/1250 August 7, 2003/1249
- 12.
Test Dates:
Auggst 5-12. 2003
- 13.
Test Type:
Short-term Chronic Definitive
- 14.
Test Species:
Fathead Minnows (Pimeyhales promelas)
Daphnids (Ceriodaphnia dubia)
- 15. Concentrations Tested (%):
Outfall 101: 10.98. 22. 43.9, 72. 100 Intake: 100 Pimephales promelas:
UV treated Outfall 101: 10.98. 22. 43.9, 72. 100 UV treated Intake: 100
- 16.
Permit Limit Endpoint (%): Outfall 101: IC,5 = 43.9%
- 17.
Test Resu
- 18.
Facility Cc Phone #:
- 19.
Consulting Its:
Outfall 101: Pimephales promelas: IC,> 100 Ceriodaphnia dubia: ICE > 100 UV treated Outfall 101: Pimephales promelas: IC,;> 100 intact:
Ann Hurt (423) 843-6714 2 Testin a Lab:
Environmental Testing Solutions. Inc.
- 5.
- 20.
Lab
Contact:
Phone #:
- 21.
Contact:
Phone #:
Jim Sumner (828) 350-9364 Cynthia L. Russell (256) 386-2755
- 22. Notes: Outfall 101 samples collected August 3-8, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were
> 100 percent. Exposure of fathead minnows and daphiiids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of int~rfere'nce (anomalous dose response and high variability among replicates) in previous topicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
2
METHODS
SUMMARY
Samples:
- 1.
Sampling Point: Outfall 101. Intake
- 2.
Sample Type: Composite
- 3.
Sample Information:
Date Date ADaeial (MCMDDY)
Sample (MM/DD/YY)/
(AMM/DD Y)/
Temp.vTl Titil me(EDTY)
ID Time (EDT)
Time (EDT)
Temp.
TRC*
Time (EDT)
Collected Received -C)
(mg/L)
Used 08/03/03 1318 to 08/05/03 1433 101 08/04/03 1218 08/05/03/ 1016 0.91
<0.10 08/06/03 1350 l08/03/03 130to 08/05/03 1335 Intake 08/04/03 1200 08/05/03/ 1016 0.9
<0.10 08/06/03 1430 101 08/05/03 1349 to 08/07/03/ 1800:
0.t
<0.10 08/07/03 1845 08/06/03 1249 08/08/03 1440 08/05/03 1325 to 08/07/03 1845 Intake 08/07/03/ 0946 0.5
<0.10 08/06/03 1225 08/08/03 1440 08/09/03 1409 101 080/314 o 08/09/03/ 0940 1.2t
<0.10 08/10/03 1416 08/08/03 1340081/342 08/09/03 1409 Intake 08/07/03 1450 to 08/09/03/ 0940 1.3
<0.10 08/10/03 1416 08/08/03 1350081/342
- TRC = TotakResidual Chlorine tCollected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
'Sample arrived late due to shipping difficulties. The EPA's maximum allowed holding time of 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> for sample's first use was not exceeded.
- 4. Sample Manipulation:
Samples collected from Outfall 101 and intake were warmed to test temperature (25.0 +/- I.0C) in a warm water bath.
Aliquots of Outfall 101 and intake samples were UV-treated through a 40-watt Smarts U Sterilizer (manufactured by Emneror Aouatics. Inc.) for 2 minutes.
3
Pimephales promelas Ceriodgphniq dubid Test Organisms:
- 1. Source:
Aquatic BioSystems, Inc.
In-house Cultures
- 2. Age:
23-25.5 -hours old
< 24-hours old Test Method Summary:
- 1. Test Conditions:
Static. Renewal Static. Renewal
- 2. Test Duration:
7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic
- 3. Control / Dilution Water: Moderately Hard Synthetic
- 4. Number of Replicates:
4
- 5. Organisms per Replicate: 10 10
- 6. Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7. Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8. Test Temperature: Outfall 101:
08/05/03-1433 EDT 08/05/03-1422 EDT 08/12/03-1416 EDT 08/12/03-1350 EDT Mean =25.1IC (24.7-25.6 0C) 08/05/03-1337 EDT 08/12/03-1243 EDT Mean = 25.20C (25:0-25.50CQ Test Temperature: UI-Treated Outfall 101:
Mean = 25.30C (24.9-25.6 C0
- 9. Physical / Chemical Measurements:
Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics:
Statistics were Rerformed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Softwvare. McKinneyville. CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1.
Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted August 5-12. 2003 using effluent collected from Outfall 101.
Test Percent Surviving Solutions (time interval used - da ys)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%-
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 98 98 98 100%
100 100 100 100 100 100 100 Intake 100 100 100 98 98 98 98 Mean Dry Weight (mg)
Test Solutions
~lct u
b
~s_____
(% Effluent) 12 3
4 Mean Control 1.093 0.962 0.964 1.046 1.016 10.98%
0.998 1.054 0.950 1.015 1.004 22%
0.895 0.969 1.095 1.131 1.023 43.9%
1.046 0.930 0.962 0.865 0.951 72%
0.944 1.035 1.218 0.893 1.023 100%
1.175 0.986 0.884 1.114 1.040 Intake 1.018 0.998 1.110 1.180 1.077 IC25syalue:. > 1000/%
Permit Limit: 43.9 Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits:
Upper Limit: NA Permit Limit: 2.3 TUc Lower Limit: NA
- TUa = 100/LC5o: TUc = 100/ IC25 5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted August 5-12. 2003 using effluent collected from Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 100 100
.100 100%
100 100 100 100 100 100 100 Reproduction (#young/femalen7 days)
Test Solutions Data (replicate number)
(% Effluent) 1 2
3 4
5 6
7 8 l 9
'10 Mean Control 33 30 33 36 30 31 36 29
- 28. 34 32.0 10.98%
36 35 31 31 30 34 35 32 33 32 32.9 22%
35 33 35 33 32 33 38 35 32 36 34.2 43.9%
29 36 33 35 32 37 31 31 32 36 33.2 72%
33 37 34 37 36 29 35 36 31 39 34.7 100%
36 35 36 31 37 37 32 36 36 36 35.2 IC2.5 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9 Permit Limit: 2.3 TUc 95% Confidence Limits:
Upper Limit: NA
-Lower Limit: NA
- TUa = 100/LC 5o: TUc = 100/ IC25 6
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2.
Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted August 5-12, 2003 using water collected from Intake.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/fimale/7 days)
Test Solutions Data (replicat number)
(% Effluent) 1 2
3 4
5 6
7 8
9 10 Mean Control 33 35 32 26 32 36 32 32 32 30 32.0 Intake 31 34 33 34 31 32 32 30 30 32 31.9 No significant difference based on Homoscedastic t-Test j
7
TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)
- 3.
Results of a Pimephales Dromelas Chronic/ 7-day Toxicity Test.
(Genus species)
(Type / Duration)
Conducted August 5-12, 2003 using effluent collected from Outfall 101(UV treated).
Test Percent Surviving Solutions (time interval used - days)
(%Effluent) 1 2
3 4
5 6
7 Control 100 100 100 100 100 100 100 10.98%
100 100 100 100 100 100 100 22%
100 100 100 100 100 100 100 43.9%
100 100 100 100 100 100 100 72%
100 100 100 100 100 100 100 100%
100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)
Test Solutions (replicate numer)
(% Effluent) 1 2
3 4
Mean Control 0.929 0.913 0.943 0.841 0.906 10.98%
1.090 1.126 1.043 1.005 1.066 22%
0.937 1.043 0.972 0.999 0.988 43.9%
1.011 1.079 0.899 1.145 1.034
-72%
1.227 1.196 1.039 1.031 1.123
^ i00R 1.090 1.121 1.266 1.018 1.124
-Inr~ke 1.191 0.928 1.092 1.060 IC25 Value: > 100%
Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits: NA Permit Limit: NA Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC25)
Pimephales promelas August 5, 2003 1215 7-days KCI 532.5 mg/L Ceriodaphnia dubia August 5, 2003 1354 7-days NaCI 1036.3 mg1L 8
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for P-inteplalespromelas and C~eriodaphnia dubia Tests, Sequoyah Nuclear Plant Elmuent (SQN), Outfall l0t. August 5412, 2003.
Test Sample ID Temperature CC)
Dissolvecd Oxy n ~(mgta/I pH U.
Conductance Alkalinity Hardness Total Residual initial FinAl Initlbl Final Initial Final (Itmhoslem) (mg(L CaCO3) (ngtL CaCO3) Chlorine (mgtL)
Control 25.3 24.9
.7.7' 7.5 7.67 7.60 311 60.0 86.0 C tol 25.1 - 25.5 24.7 - 25.6 '7.5 "7 7.3 7.8 7.39 - 8.04 7.41 - 7.89 302
- 321 59 61 84 8
1098V
~25.3 24.9 7.5 7.63 7.56 297 109% 25.1 - 25.5 24.7 - 25.6 7..6
. t.2 7.2 7.8 7.38 8.01 7.37 - 7.87 282 - 309 22% ~
25.4.
24.9 7.8 7.4 7.63 7.55 287--
_____25.2
- 25.5 24.7 -.25.6 7.6 8,.2 7.0 7.7 7.37 - 8.01 7.34 - 7.86 276
-295 43.9%
25.4 24.9 7.8 7.4 7.59 7.53 259--
_____25.2
- 25.5 24.7 - 25.6 7.6....
8.2 7.0 7.7 7.31 8.00 7.31 - 7.83 251
- 266 25.4 24.9
.7.8 7.4 7.55 7.53 221--
__72___
25.3 - 25.5 24.7 - 25.6 7.6 8.2 7.0 7.7 7.25 - 7.99 7.31 - 7.85 215
-227 25.4 24.9 7.9 7.4 7.51 7.54 184 63.0 74.7
< 0.10 10% 25.3 - 25.5 24.7 - 25.6 7.7 8.2 7.0 7.7 7.16 - 7.98 7.28 - 7.90 180 -
191 61 65 74 76
<0.10 -
<0.10 Intake 25.3 24.9
- 7.
7.4 7.54 7.55 180 62.0 75.3
< 0.10 25.1 - 25.5 24.7 - 25.6 7.7 -
8.2 7.1-7.8 7.42 - 7.97 7.35 - 7.90 174 185 61 63 72 78
<0.10 -
<0.10 Cotrl 25.3 25.1 7.7 7.8 7.67 7.70 311 60.0 86.0 25.1 - 25.5 25.0 - 25.3 7.5
-7.9 7.5 8.0 7.39 - 8.04 7.50 - 7.97 302
- 321 59 61 84 88 10.98Y 25.3 25.1 7.8 7.8 7.63 7.70 297 25.1
. 25.5 25.0 - 25.3 7.6 8.2 7.6 8.0 7.38 - 8.01 7.49 - 7.98 282
- 309 25.3 25.1 7.8 7.8 7.63 7.70 287 22%___
25.1 - 25.5 25.0 - 25.3 7.6 8.2 7.6 8.0 7.37 - 8.01 7.49 - 7.96 276 295 25.3 25.1 7.8 7.8 7.59 7.70 259 Q.
~~25.2 - 25.5 25.0 - 25.3 7.6
-8.2 7.5 8.0 7.31
- 8.00 7.51 - 7.97 251
- 266 72V 25.3 25.1 7.8 7.8 7.55 7.72 221 25.2 25.5 125.0 - 25.3 7.6
-8.2 7.5 8.0 7.25 - 7.99 7.51 - 7.98 215 - 227 25.3 25.1 7.9 7.8 7.51 7.73 184 63.0 74.7
< 0.10 100%
25.2 - 25.5 25.0 - 25.3 7.7 8.2 7.5 8.0 7.16 7.98 7.52
- 7.98 180 191 61 65 74 76
<0.10
<0.10 Intake
~25.3 25.1 7.9
- 7.8 7.54 7.70 I80 62.0 75.3
<01<0.10
___Inta
- 25.
- 25.5 25.0 - 25.3 7.7 8.2 75-8.0 7.42 - 7.97 7.52 - 8.01 74 85 61 63 72 7
01 01 Overall temperature (CC)
Pirnephales prornelas.
Ceriodaplhnla dlubia Average 25.1I 25.2 Minimum 24.7 25.0 Maximum 25.6 25.5 9
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pimephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, August 5-12, 2003.
Test Sample ID Temper ture(C)
Dissolved Oxygen (mg/L) pH (S.U.)
Conductance Initial Final Initial Final Initial Final
(^Imhos/cm) 25.4 25.2 7.8 7.5 7.63 7.57 303 Control__
24.9 - 25.6 25.0 - 25.5 7.7 8.0 7.2 -
7.8 7.50 - 7.97 7.35 - 7.88 292 -
314 10.98 25.125.4
=
25.2 7.8 7.4 7.64 7.55 297 25.1 - 25.6 25.0 -
25.5 7.6 8.0 7.0 7.7 7.50 -
7.97 7.30 - 7.85 286 -
305
> 22%
25.4 25.2 7.8 7.4 7.63 7.54 285 25.1 - 25.6 25.0 -
25.5 7.5 8.0 7.0 7.6 7.49 - 7.97 7.31 - 7.84 275 -
292 A 43.9%
25.5 25.2 7.8 7.3 7.61 7.52 258 m
25.2 - 25.6 25.0 - 25.6 7.5 8.0 6.4 7.6 7.46 - 7.97 7.14 - 7.85 251 264 i 72%
25.5 25.2 7.8 7.4 7.59 7.53 220 72%
25.2 - 25.6 25.0 - 25.6 7.5 8.0 6.9 7.6 7.42 - 7.98 7.27 -
7.87 215 226 4;;
100%/
25.5 25.2 7.8 7.4 7.55 7.54 184 25.2 - 25.6 25.0 - 25.6 7.5 -
8.0 7.1 7.7 7.36 - 7.96 7.29 -
7.87 180 -
187 Intake 25.5 25.2 7.8 7.4 7.53 7.53 180 Intake__
__25.2 -
25.6 25.0 - 25.6 7.5 -
8.0 7.1 7.6 7.32 - 7.95 7.28 -
7.85 177 -
183 Overall temperature CC)
Pirnephales promelas Average 25.3 Minimum 24.9 Maximum 25.6 10
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected August 3-8, 2003, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples, suggesting that pathogenic bacteria were not present.
a 11
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1.
Pimephales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2.
Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1.
Pimephales promelas None
- 2.
Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS,
- 1.
Pimephaqh. promelas None
- 2.
Ceriodaphnia dubia None 12
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Total Hardness was measured EPA Method 130.2.
OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study-and are reported herein.
REFERENCE YOX ICANT TESTS (See Appendix D for control chart information).
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCI or NaCl.
- 2.
Standard Toxicant:
Potassium Chloride (KCl crystalline) for Pimephales promelas.
Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4.
Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
13
REFERENCES
- 1. NPDES Permit No. TN 0026450.
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPAI600/4-79/020 (March 1983).
/,^a.
14
Sequoyah Nuclear Plant Biomonitoring August 5-12, 2003 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,
,8
-*N,.
15
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 8, 2003 16
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 8, 2003 Towerbrom mg/L TRC
- 4.
p 4.
'C-
< 0.002 0.011 0.028 0.008 0.006
< 0.002
< 0.002 02/05/2000 02/06/2000 07/27/2000 0.019 0
O.55 07/28/2000 0.0088 0.053 07/29/2000
< 0.0088 OS 55 07/30/2000
<0.0076.
`6 055s,;
07/31/2000
<00152 4s 08/01/2000 V
< 0.0141
°00 12/11/2000 ~~
~0.0143
&'0.025, 12/12/2000.
0.0092 0.0;5 12/13/2000
< 0.0120 0,025.^
12/14/2000 3
0 087 6O5 12/15/2000 0.0120
.2 12/16/2000
.~
<0 00036
.~2 12/17/2000
< 00036 02 08/26/2001 0.017
.0..
08/27/2001 :
0
<0.0096 Or.0.06-08/28/2001
<0.0085
-AM`6 t 08/29/00 1
.'<00094
.059 08/302c0 ~-
~<0.0123 0.O06,A
08/31/2001
<0.005 60.059<.
11/25/2001
<0.0044 11/26/2001 f
<0.0119 6-.024 11/27/2001 0.0137 iQ.023 11/28/2001
<0.0089 0.022 11/29/2001 y 0.0132 0.024 11/30/2001
< 0.0043 0.024 -
12/09/2001
<0.0042 12/10/2001
<0.0042
< - A 12/11/2001 7 -.
<0.0104 12/12/2001 L, 0.0128 0.024 12/13/2001
<0.0088 0.024 12/14/2001 r
0.0134 0.024 17
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 8, 2003 18
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 8, 2003 19
Sequoyah Nuclear Plant Biomonitoring August 5-12, 2003 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
.-I a.
BIOMONITORING CHAIN OF CUSTODY RECORD Environmental Testing Solutions, LLC Research Building Delivered By (Circle One):
UPS Bus CHi Other (speci): _
int Facility Sampled: SEQUOYAH NUCLEAR P NPDES Number: 0026450 351 DEPOT STREET ASTEVILLE. NC 28801 LANT Phore:- 8.28-350-9364 Fax:
256-386-2963 Container Collection Date/Time Number&
Flow GerLeral Comments: ;
C V
I i
I I:
do Collected By: WANDA ALLEN Field Identification /
Sample Description SQN-101-TOX
- Fill In F S
A__
rom I op Down l
Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time k&6,Ld1Lv a&s_
8/4/03 sow FED EX 8/4/03 ramtQL lolbf
- M-5 l0l o Cs-as lo Instructions
- Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> rrom the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
i i !
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Environmental Testing Solutions, Inc.
Whole Effluent Toxicity Sample Receipt Log Date Tim Received Received Sample Project Sample Sample name end description State Comments received received by from temperature number number I
0105-03 1016 J. Sumner FedEx
,4.'
739 030105.01 CORR ERI -McQusy Intanational, Afla aw pper AL 01-05-03 1016 J. Sumner FedEx 47Q 379 030805.02 CORR ERI - McQuay International After nwat.
NC 0Ot05-03 1016 J. Sumner FedEx
.:i.5 790 030t05.03 Progess Enery Carolina - Sunon Plant NC Ot05-03 1016 J. Sumner FedEx Q.9 791 030105.04 TVA, Sequcyah Nuclear Plnt -Outfall 101 TN 08.05-03 1016 J. Sumner FedEx 0.9 791 030305.05 TVA, Sequoyah Nuclear Plant - Intake TN O3-05-03 1016 J. Sumner FedEx 1.2. '
792 030305.06 TVA, Gags"in Fosi Plant -Outfall 001 TN 0-5-03 1016 J. Sumner FedEx 1.2.
792 030305.07 TVA, OaGlhti" Fossil Plant - Intake TN 03-05-03 3341 J. Sunner L Coward 0.7 793 030S05.01 Blue Ridge Patkway -ML Pisgah Lodge NC 03-05-03 1435 J. Sumner Asheville Coudier 0.2 794 030305.09 Spnicc Pine WWTP.
NC 01t05-03 1435 J. Sumner AsUeville Courier 0.4 795 030105.10 Baxter Healthcare Corporation NC 0305-03 1435 J. Sumner Asheville Courier 0.9 796 030805.11 CommScope, Inc.
NC 03-01-03 3435 J. Sumner Asheville Counier 0.6 797 030305.12 Waynesville WWTP NC 03-05-03 1634 J. Sumner A+ Deively 0.3 79t 030305.13 TnTc t, Inc. - Apex WWTP NC 0-OS1-03 1634 J. Sumner A+ Defivety 0.3 799 030305.14 Tnuest, Inc. - OWASA Muon Frms NC
~~~~*1,~~~~~~~~1 i
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BIOMONITORING CHAIN OF CUSTODY RE CORD Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
.)UPS.
Bus Name: TVA SQN Research Building P.O. Number: PO BOX 2000 351 DEPOT STREET
\\ X.ASHEVILLE. NC 28801 Phones. 828-350-9364 A0P Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT I
NPDES Number: 0026450 Fnx.'a 256-386-2963 1 )
General Comments:
I
'l I I
i Ice Collected By: WANDA ALLEN 5)
Field Identification /
Sample Description Collection Date/Time Container Number &
Volume
('nllewt*.
8/6/03 of50C Instructions: Clients should till in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them ft'4'C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Thereibre, please collect and ship in such a way that the laboratory will receive the samples with ampil time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. I1.!.
[
I.
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SQN Toxicity Samples 8/6/2003 Toxicity samples were pulled off and packaged into 3 coolers by Wanda Allen and Ronnie Hankins. Wanda Allen sealed these 3 coolers with COC seals, and gave them to David Porter to deliver to FedEx. David delivered 1 cooler (intake sanple) to FedEx and the other 2 coolers (diffuser samples) to TVA Central Labs instead. Lewis O'Rear at TVA Central Labs received the 2 coolers. Lewis broke the COC seals and realized the samples were not TVA Central Labs and placed the 2 diffuser coolers into the walk-in cooler at TVA Central Labs.
8/7/2003 Wanda Allen and Ronnie Hankins went to TVA Central Labs and retrieved the 2 coolers from Lewis O'Rear. The diffuser samples were still packed in ice in the original coolers and the coolers had been placed in TVA Central Labs walk-in cooler all night. Wanda Allen re-iced the samples and re-sealed them with new COC seals and gave the 2 sealed coolers to Ann Hurt. Ann Hurt relinquished the 2 coolers to Sonic Delivery to be delivered to Kelly Keenan at ETS in Ashville, North Carolina. Kelly received the 2 coolers from Sonic Delivery the evening of 8/7/03.
8/14/2003 Ann Hurt Environmental Scientist TVA Sequoyah Nuclear Plant
., ~
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I JIOMONTORMG CHAIN F CUSTODY RECRD I'Age _. L of 1
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Cient: TVA EnvironmentsA Testing Solntibns, LLC 48ame: TVA ReseArch Building Dolhvtd By (Ctele Onej:
)ws UP u
d~iet
- Ohrfeq, ae P.O. Nuhber: Po BOX 2000
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AS 1VLL NC 28801 rhontb:.8 8,509364 Faility Sampled: SEQUOYAHRUMLEARPLANT I
NPDES Nwnbe. 0026450 r.hi: -- 8Z-&,0V EZ8-850 - feeV8
.. /
Gepral Commenl:i.
-lk CL~aOS
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Collected Dr WANDA ALLEN
.6 Plddldenaticatidn/
SampleDescription I
i
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nstbuctins: Clients should Ml in all areas cxiept those in the 'ILebtory Une bloc.
Eiwitoring smplks are am escved by etoin bAœ at C: nd Abins them it ice.
e old time for each mnple is 36 ho1rs from the fime of collection. Thmfm please collect and ship in, sect a wy (hat the labofaty rill receiv the 1mles witk e*pk #m to initiate testiug wiihin that tim rae.
Sanples shipped ovenight on Friday via Fed&x or UPS must be nutked for Saturday deliveyor (hey wll not Knive until the foin(owki MondY.
I
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Environmental Testing Solutions, Inc.
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Page rA Whole Effluent Toxicity Sample Receipt Log Date Time Received Received Saple roject Sample Sample same and description Stat Coments received received brei t
IU!rt
. number number 08-07.03 0946 K. Kuonn FedEx
.0.3 789 030807.0I CORR ERI. McQuay i At Mer si tripe r
A_
0847-03 0946 Il IKeen FcdEx
-0.3 739 030907.02 CORR ERI - M n tnantionI Aer mle AL 0S07403 0946 K K FedEx 0.4 Ri0 030807.
Due03 iEnery Cption McOuieNucImrISuttion Outfill 002 NC 0Ot0703 0946 K. Kena FdEs
- n.
030807.04
'CS P ih Coeny. InM.
NCOutal 007 NC 06.07.03 0946 1K. Kean FedEx S
030S07.05 PCSPl Company. Inc.
1 Ouall 100 NC
)
08.0743 0946
- 1. Suciui Fedxi 0.5 791 030607.06 TVA -S quoyah Nuclear Plnl, Intake
_TN__=__
084743 1436
- 1. S.5 K. Kemn 3.7 1 2 030807.07 Teat Anicna. Ine.. Eegth P2n
-egl Sevicen MihOH WWTP NC 0O47.03 1800 J.SOumw
.Maie-Sank Delivery 0.6.
791 030607.06 TVA.SoquoyahNuclear PlanOutflll 101 TN I
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BIOMONITORING CHAIN OF CUSTODY RECORD Page I
of I
Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building UPS Bus Client 351 DEPOT STRIEET P.O. Number: PO BOX 2000 351 ASEVLT NCR28801Other (specify),
ASHIEVILLE. NC 28801 _
Phone; - 828-350-9364 Facility Sampled: SEQUOYAH NUCLEAR PLANT P
Geneial Comments: k NPDES Number: 0026450 Fax;:: 256-386-2963
+ C-i;4' Collected By. WANDA ALLEN i
.1, s Container _
Field Identification /
Grab/
Ship.
Collection Date/Time Number &
now Rin Event?
I Sample Description Camp Temp.
(
VolmcM (MOD)
(ark as Appropriate)
______Collected Ii~t,
~..
Start End Y-if Yesk No Trace
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X.A***~
SQN-l-TOX camp 4
. Ig4D 8/8/03 N.
-i SQN-101-TOX S/7103 M
2(2.5g)
I
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8/8/03
~*
SQN-INT-TOX comp 4
8/7/03 8/810 I (2.5g) 1 i
Z I=========
=_____
7Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
j, j
Date/Time Ita/LuzeL ate..Ln-8/8/03
/ - FED EX 8/8/03 F~L 8.dq-O3 d LtO e0.
oe
,1-0:3 0
/v istructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4 C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Tlherefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be markled tor Saturday delivery or they will not arrive until the following Monday.
I i
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Environmental Testling Solutions, Inc.
Pace WVhole Eltuent Toxicity Snmple Receipt Log Date Time Received Received Sample Project Sample Sample name and deacriplion State C anoents ecIved received by, rom teswperashC) number number 0-0943 0940 K. Keene FedEx
.. 1.2 s 791 030809.01 TVA, Se h Nuclear Plot
- Outfail 101 TN 0O-09403 0940 K
C Kea"n FedRic
- V 7 91 030309.02 TVA, Sequoyah Nuciat Plant. inbeh TN Ot0943 0940 K. Keeli n FedEx
,, 0.2 313 030809.03 Hercules, Inc.
CA 08-0943 0942 L Keelna FedEx 3.7 303 030809.04 Duke Eneg Corporation -BDelew Creek Steamn Station NC 0o-09w03 1035 K. Keenan i ihue m
0.41 1 799 030309.05 TniTeht. in. -OWASA Mason Finns NC 0Ot09-03 1035 J. Sumner WiNWhtaue OA.
79t 030809.0t TniTest nc. -Apex WWTP NC 0809403 1035 J. Suner lUihowe 0"4' 307 030309.07 TriTeat. Inc. -Wilson WWTP NC 08409403 2200 J. Swaner Greyhound 04l 792 030309.08 TVA G llatin Foesil Plant. Outili 001 TN t09403 2200
- 1. Sumner Greyhound 0.4 792 030309.09 TVA. Gallatin' Fosil Plant. intake TN iI 1 i I
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. Environmental Testing Solutions, LLC Page 1 of 6 Eniomna Tetn SouinLC ae1o Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant Nontreated NPDES #: TN 0026450
-.~~~ ~ ~~ _Proqject'#:-- -°; l "~~ ~~-~~-
.C.-
Dilution preparation information: -
- Comments:
Dilution p
p "K.
I.0^t I-z.
Iz l
II I
Effluantvolume (mL) I i'A I 4't-i I ti I lfl440-- 1a6,c 1
lDilue"'volume(mL)
IEt&.1A1 M O IlIm I S, l o
I 1
ToUl volume (niL) l 2C JO OO0 l_ZO__I__o______
Test organism information: Z3 Zs.S T est information:
Organism age:
w
,4o<jves OON Randomizing template:
%[*L Date and times organisms Ot -
-C53
-rt>
Incubator number:3 were born between:
Inub
-ator3number Organism source:
F86& 6grik Ot-oq-qC Arremia lot number:
3 s Transfer bowl information:
pH =
Temperature =
°C Total drying time:.
I-paS 1410
-Z Pq.
C Date / Time in: a.142
%Soo Average transfer volume:
-Date / Time out: OR105 O~Oo
.0.4V Oven temperature:
10o-C Dailyfeeding and renewal information:
Day
,pate
.,Morning Afternoon Test initiation, Control water Sample numbers Analyst j '.feedtig. feeding renewal, or batch used used
- time time termination time 14 IS
° oe as I
&tt.
3 o!,&t M
5 WK.
OY Ss 69 06a os3 MeOO ISC6 1-656 O la a 41-6S dR
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6 rJ4
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- 12.
0t 0ft-01 0101^0'i i
7 Poft tl Control information:
Acc.ptune cntena Summary of test endpoints:
% Mortality:
c
- 1.
S20Y%
7-day LCso 7
1 t
Average weight per initial larvae:
I.0((.Z iOEC Average weight per surviving larvae:
I.0 lZ.
2 0.25 mglarvae LOEC
-' >lOO ChV
'S 07r IC-Is
>16010
Environmental Testing Solutions, LLC Page 2 of 6 Species: Pimep hales promelas Client: TVA / Sequoyah Nuclear Plant Nontreated Date:
0OA CJ*SC2b Survival and Growth Data Day
-CONTROL.1.
8%-
A -
B C
D E -
F
- -G
--H I
--J--K -L--.
.- ° _1_
-t.
o -
/0
/0 Z
/0
/0 D0
/-
/0 10___
1 lC l° 1,0 0o
/0 IC.) lo lo 10, t 101' 2
/0/010( /0
/
0 /<
/()
/6
/
_/0
/0 3
/0/0 //0 0 (0 t
/6/6 /0 to(to (0
/0 to (0/
/t0
/0 10
/0 /01 1/) 1 10
/cO to I
lo c lM l
1 /
0-I I 0 1'-
I0
/0 JO 6
lo_ lb 10 lb
_ l
/0iD
/0
/0 0 /16 /0
/0 7___
/()
1b
/i
/
/6_-
0t
/0 le) /0 A - Pan welght emg
_(_
_T
\\
4 4
A, A b'
° B -Pan ljrar~Ltjregh"Fng)
B u r,hv~~~nda.
- t.
0
.0,Ak1^
/
1 of Larvae weight(
-A-B D
- 4) k P
E 43*
-Cl Initial nuznberoflarvae
)
.0 O'.....
0 e
VO v
O Average wdght per in etlal numberorlarvae(mg) l * °o'4Z-V.
czzl Calculations and data revieNved:
Environmental Testing Solutions, LLC Page 3 of 6 Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant Date:
pt. CG-03 Nontreated A-
_Survival and Growth Data
.Day -
~~~~43.9%
72%
AOO%
N 0
Q R
S
-T U
--V
-M
_x
-- -~~~~
Ic)
/0
/0
(~~~~~0 to. 1
/0 JO -10 1 JO -16. /0 1
10~~/0 IC>)0 1 I A) 1/O O )
1/
IQA)bo
/O 2
lc~~~~~~~~/6l10 /O 0/
/O /O J0110 I
______3 to/0 Ot 10 Ic 0
0
/6/
/O. /O 0(
/0/I0 1/O
/0 /O
/6/
/to
/6O '
6
/0/0~~~~~
/6
/O /0/O /6 lb/ /6 /0 /
1~~~~~~~oI01to10/0/0 O
110to/0/0/0 A - Pani weight (mg)
I B-Pan *,~vawIgbt (mg) f V
Wegtpental Nei
'VIi A
~
4t 4:
Larvae weg(in g)
-1 AegeWeight per Initial ubrrA Z
number of larvae (mg)
- 0. is I
. OzzSBo Calculations and data reviewed:
Comments:~~~~~~~~~~~~~~~~~
.. Environmental Testinp-Solutions, LLC Page 4 of 6 Eni; mna
_etn ouin 1 LCPg r
Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant Nontreated
-Date: Ot-OS-C53 V.
I.
1*
I I
Survival ansd Grnwth. Dafii -
Day 100% Intake
- Y Z
AA BB 0
0' 10 /0
/
2 A
(6
/6
/0 3
/0
/6t
/0 4
9, I, 11)
/)
6 l/
A-Pan weight (mg) t B - Pan + Larvae weight (mtg)
Larfae weight (mg) - A-B Weight per Inithal number of di larvae (mug)
~4~
- C / Inltal number of larvae Average weight per initial number oflarvae (m)g)
P.
a.
I.
Calculations and data reviewed: 4A Comments:
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Environmental Testing Solutions, Inc.
i Chronic-Whole EMuent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas
\\
\\.
Quality Control Verifikatioht 9ata Entry, Calculations, and Statistical Analyses' IVA Sequsysh Nuclear Plant. Nontrtated Client:
Test datesr Project nuntbae:
August 5-2,2003 791 I
II I
I I
II '
I Revehead by-,
-.4r Cooccatbade %)
Reopiate khdaui sberam d`
blR A -
we gh1t m-ra
- a.
L webht Cm) W dl E1 sb Mea ssartvul Me wdeiht (me)
Ceftdekt do Perceat reducte from tame w4ht (m)
-A-B brvae(mg)
)
vatle"
(%)
csu'a
)
A 10 l0 15.150 26.080 10.930 1.0930 Conttol C
t0 10 14.996 24.620 9.624 0.9624 100.6 1.01o2
- 6.
Not ppitb b
Control 100 to 1073324.210N9t637p0.9a37e D
10 10 14.253 25.310 10.457 1.0457 K
310 30 15.271 25.250 9.979 0.9979 1.°t8 CF 10 10 15.111 2 25.720 10.539 1.0539 00:.00*42 4.3 1.2 18.989A a
1o 30 14.715
.24.210 9.A95 0.9495 000 H
10 10 14.597 24.750 10.153 1.0153 I
10 10 15.046 24.000
.954 0.8954, J
0 30 14.701 24.390 9.689 0.9689 K
10 10 14.988 25.940 10.952 1.0952 100.0 1.6227 10.7
-0.6 L
10 30 15.00t 26.320 11.312 1.1312 Nt 10 t
14.996 25.460 I1.464 1.0464 N
10 10 14.727 24.030 9.303 0.9303 100.0 0.95t 7.9 6.4 o
io 10 14.989 24.610 9.621 0.9621 P
o 10 135.076 23.730 8.654 0.8654 O
10 10 14.642 24.080 9.438 0.9438 n20A s
10 t-o 15.167 25.520 10.353 1.0353 975
.022S 14.0
-0.6 S-10 30 14.331 27.010
.12.179 1.2179971021.006 T
30 9
15.020 23.950 8.930 0.8930 U
30 10 15.056 26.t10 11.754 1.1754 100%
V 10 10 15.058 24.920 9.862 0.9162 1000 10399 12s
-2 W
10 10 15.070 23.910 1.840 0.8840 1
-23 X
10 10 14.909 26.050 11.141 1.1141
.U 10 9
14.656 24.t40 10.1U4 1.0184 100% Intake V
10 30 14.939 24.920 9.9tl 0.99-13
- 97.
1.0768 7.9.
-L0 X
t0 10 15.047 26.130 11.103 1.1103 X
10 10 14.636 26.440 11.804 1.1804 OutrN11101:.
Dunnett's MSM valust
- PMSD.
Intake:
Dunnetvs MSD yalue.
l'MSD:
PMSDO 0.17213 16.9 PercatMinimxn Significant Differenei P
SD is a nuKesof taest preclsion. The PMSD is the nininmaporcut difat ta thcntrl end tratnnt tht can be declared statistically significut in a whole cluant tesicity teL On simmp a significant diffence ocern for &E bicirxt Tcting Solution kmc.
chronic toxicity tests when a toxicant reduces Pirnaphales growth by 16.7% fIlir the control (defacined through refcernce toxticant testina Lower PMSD bound detennined by ULEPA(lOth paecntile) - 9.4%.
1 1
LkPe PMSD bouwd detarninedby UEPA(9Oth paeretilc)- 35%
'The lwer and upper bounds wetrecaslculted by the U5EPA uwing 205 tet conducted frntn 19 tabcratories ror Pinesphalea rwmh in chronic refernce toxicant tests.
0.1032 10.2 I
USEP 2000. Understanding and Accounting for Mcthod Variability in Whole EFlulent Toxicity Applications Under the National Pollutant Discharge Elirninstion Provnel EPA-133-R4.003. US En rwmttal Pmntection Agncy.
, Cincinnati. OK Il
Environmental Testing Solutions, Inc.
Statistical Analyses Loala Flak Crowta mand Survival Text.7 Day Growels SWai Dom: W5103 Tast [D~.
PPFRCP.
aUnple ID.
TVA.Scquwoye Hud lcant~v.
outranlot20 Led Dae:c V12103 Lab 1D.
MEnSEs.
Toxf Sohdtona SawpleT*~v DMR.Cischurg Moaiitoafr RaPoft Sampl Dazc PmotocoL-CHROMC.(EPA4221.ft.0143)
Test Species:
PP.Pimephates prowcliu CAnUnat: w...b-eraad CeaD-%
I 2
3 4
D.contko 13930 0364 03637 2.043 2036 1997 1.0339 0,9493 LotsS 22 0.8934 0939 2.0952 1.1312 43.9 1.0464 19303 09621 09634 72 GNU3 1.0333 12179 0.81930 100 1.1734 0362 014 1.1141
-Tnasenn:
Um uufne 1-Taie Isteu Ceuc-%
Mean N.Man M"t MI M
CVS 4
Utat Critical MSD Mman
-Mean Dle ulQ 1.0162
[M0
.0
-03624 1.930
.63 2
4 1.0162 1.A01c 10.9o I042 0.98I Ieoa4 0.9493 1.0339 4.32 4
0.169
.410 M1721 1.0134 0.9973 22 1.027 1.0064 1.02t7 0S954 1.1311 10.731 4
4.091 2.410 0Q1721 1.0134
.s973 43.9 -
-0.9511 0.9339 0.8654 61.04 ---.
7.911 4
2.40 0.1721 3S.
.063 12 1022 1.0062
.22 0.8930 t.2179 U961 4
4.0Ft 2.410 0721 Low 0.96ts 100 1.0399
.23 2.0399 0140 1.1734 2L2.45 4
4=33 2.410 0.1721 l.045 0.9ss Shaplzo.Wi*fs Talt hidicates aouaial disaibutOo p.01) 0972n3743 0t34 0.237229027
.043334799 ardetfsTestbudleata eulvanaa(P.'0.44) 40232249 1380631706 Hypothuis Test (lUt, 8.05)
-OEC WEC ChV TU M.SDu 865 438 53 63 IFProb if Dfunews Tat 100
>I00 I
0.172069063 0.169325 0QC038565058 0010195334 0.860294378 3 is Tretanents Vs DControl Uuee tluterpelsto (200 Reaples)
Fewnt SD 95% C4EXiu)
Skew IcOS
~~~~~1l00 ICIS
)100 I=
100 0
tC10
>100 1c23
~~~~2100 IC30
'200 0.
0..
0 0.3 02 0
.0.,,,,---'
' n&e o
0 20 40 80 s8 100 120 Dose %
DoseResponse Plot
- 1 -taflL 0.05 level of significance
.sqnOJJS.D03dotarlfs
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growthi and Survival Test-7 Day Growth Stan Date:
VWlO Test ID PpFRCR Sample V~
TVASquoyahi Nuclear Pla Intake End Date:
Vll2103 Lab ID:
ETS-Eny. Testing Solutions Sampe Type:
DIAR-Discharlie Montoig epait Sample Date
~
Protocl: -
CHRONIC.(EPA.321.R.02.013) ~.Test Species:
PP-PimePhalespromelas Conmnents: Non-treated.
Cone-%
1 2
3 4
D-Control 1.0930 0.9624 0.9637
-1.0457 100 1.0114 0.9981 1.1103 1.1304 Transfora: Untransformed I-Tailed Cone.%*
Mean N-Mean Mean Min Mal CY%
N t-Stat Critical MSD D-Cantrol 1.0162 1.0000 1.0162 0.9624 1.0930 6.332 4
100 1.0768 1.0596 1.0768 0.9911 1.1304 7.354 4
.1.141 1.943 0.1032 Auxiliary Tests Statistic Critical skew IKurt Shapiuo-Wilks Test indicates mnoral distrilwtion (p->0.01) 0.1826193 0.749 0.36270522 -1.70952182 F-Test indicates equal variances (p -0.66) 1.727921883 47.4613456 Hypothedis Test (1-tall, 0.05)
MSDu M15Dp MSB bl51 F-Nrob dr Homoscedastic t Test indicates no significant differences 0.10324894 0.10160297 0.00734472 0.00564644 0.29754323 1.6 Treatmewus vs D.Controt Dose-Response Plot 1.4-I -
0.4 D.0.2 4
- a.
- a..
I-t Ang0
... I......................................
-of $age I
ni i;;;e C I I
sqnO08-01-03data~x~f
Envirehmerital Testing Solutions, LLC Page I of 7 Eniomna Tetn SouinLCPg f
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphnia dubia Client: TVA Facility: Senuovah Nuclear Plant NPDES f: TN 0026450
_- Project #:
7 Dilution preparction information:
Comments:
Dilutionprp(%)
prepI zz
___________I_____-_
Effluentvolume(mL) ziiA ['
dlV 1i40 Z
Dilu volum (L) 8 Si tlZZ SlOI C)
Total volume (mL) 2C1 j2 Z _
Test organism informeation:
est information:
Organisn age:
4 7AI -jI 1 Ls5 Cnr Randomizing template:
GftIeeJ Date and times organisms
- 0. OS. az.
3Z r, Iz Ci Incubator number and were born between:
shelf location:
Z Organism source:
Cal-Z-63 A-IP YCT batch:
`i1fss O1Z263 Transfer bowl information:
pH =1. VS Temperature = ZS.O Selenastrum batch: Ae Il-22-CS Daily renewal information:
Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time HIEt
-o atsc 1331 0
1 -s1-.
I o (WOs.S 1.0 I
o a l3 ZS O CA\\->
P-c h Ob t0O I.QS t
6 ^
aj~n, l35 O#-ot3 adoro%.
-a At.
R 2 -
_0_1 Control infornuit
_-u_
l_
etance ci Su aot0d.0o1i ts:
o OM A
ts:
fl.IS
.C) GR-S20 7-ofday lCSO
Ž
-l% Adlt havin 3" Bods NO EC Men7fsrig IeMae b£
-1.0s lsi3mat
_-V Control information:
Acceptance crtia Summary of e2 cndpoints:
% ofMale Adults:
S~ 20%
7-day LC50
- > I YS YO Adults having 3rdBroods
I tD<
0%
NOEC L W'7
% Mortality:
i S '
s20%
LOEC
>It1 Muan Offspringe/Female:
32O a 15.0 ol~prngfremale ChV
> llo&
% CV:
t-1;<
40.0 %
IC25
> l M%
Environmental Testing Solutions, LLC Page 2 of 7 Species: Ceriodaphnia dubia Date:
g
-oas Client: Sequoyah Nuclear Plant CONTROL-%
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 I
Youngpoduced 0
0 O
LCI l
Adult mortall L-L L_
2
-Young produced 1 - O -Ic I c 1 o CO l
dlt mortali t 3
Young produced O
-)
O -UC3 0
0 IAdult mortalityq C_ l
-U--jL-4 Young produced g
j4 5S
- Adultmortallty i;x L-1 '-
l l-Sl Young produced 0
C_
l CO lAdult mortailty l_ll\\ _l\\,tltl>l>l 6
lYoung produced zlt1° lC I
lt
-2 C3 l tO-0 Adult mortality k I w! L !
_ K~~~~~\\-
H_
__I 7
l Youngproduced [I'sO 15 15 I IS I'S' I l I£ Ijl Totalyoungproduced I 35 s 1 33 T 5 T 30T 3\\ I l 29 l 2& T tA Final Adult Mortality l l l l 1
L_
T? t_
I I
I X tor 3' Broods
+/- Ze+/- I +/- l
.C l
>.ŽlNC:t Concentration:
-% Mortality:
t I
Mean Offspring/Female:
132.0 j CONC: 10.98%
Survival andReproduction Data
- l.
l______
Replicate number I
l Day '/o I$.
l 1 l 2
l-3 l
4 l
5 6
7 l
8 9
110 l1 T Yeung procduced, l C )l(
Ol^l{
I____
- _LL, L-I L l
2 Yodng produced Cf)f i
I o
o i
Io o IC) I*h Adult mortality L
L-lr-3 Young produced l W
l ()
l 010 l
l()
1 XL I O I
Adultmortality L
j L
I l.1 1
L 4
Young produced C
j T
1a 1i SI i oiSiS Adult mortality I
[
I
=
_~
i d s
Young produced I'10T
~
V~
'4" ILITW]6
!, I T I
1Adult mortality X-_z L'-I I `U.,
6 l Young.produced C)..
I 0
(
"Ti 01 C)
Y a
J Adult mortality l_
l L-l l
'l L L l
I l
7 Young produced Il2Ll I(p l
l z
l
- 2.
- 1. 6 is 1
1 11 I S Totalyoungproduced l 36 1 3 l
3\\ l.30 l 4 l Final Adult Mortality C
l L
l I
I
-U t_
l Concentration:
%Mortalit:
MO I Mean Offsvring/Female:
.l 32.9 I
! % Reduction from Control: -2.i l
Environmental Testing Solutions, LLC Page 3 of 7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant Date:
ad - bS -0?,
CONC: 22%
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
1 9
10 IlYoung produced 0
O OOO<
Adult mortality 1,
L l
1 L-1 1
2 Young produced o
0 C
O 0 tO IAdult mortality I
I L 3
Young produced 1
0 O
1 I
o o
I Adult mortality l
[ '.-
WFC4T?
l L
U-I '-Il --
4 Young produced 1
1 I
r Adult mortality U-L1 L
S Young produced C)0ilO ll5 i
16
%b V21 51t ultmortlit L
A lW
'1 l
L, 6j Young produced 13la l I
_CO l
Adult mortality 1c..
_ T1 u
I l
1 -_
I s l *.
,Li Young produced k
I J 1 L
i
ŽL J I"
S J l
Totalyoung produced l
l3 3S T 3
3asi a
t I
3 Final Adult Mortality l
Z I
4 I
C 3 W
U 1 -
Concentration:
% Mortality:
I CJ5 MeanOffspring/Female:
l
% Reduction from Control: 1
- I CONC: 43.9%
Survival and Reproduction Data I___i_
Replicate number
_Day 1 2 3
4 S 1 6 7 1 8
9 TI lo l__
1-0 l
D I (I
_'4g e~d
_ViL K[___
z _
L U
Adultmonrtality V.
L-l I_
U.
ll 2
Young produced l
ll°Ql l
I Adult mortallty l
U117_____________
3 Young produced j
L i
(
i(O l
l l
0 l5 ult mortality 6
Young produced I
j Adult mortallty l__
I___
L l
1 U I l
I l l Adult mortallty L
L L
-- L l -
L l l 7 l Young produced l
z l
1l l -b 1 b I'S l N1-1 I 1 ILA I 1-I I -1 Totalyoungproduced It l 4i I 3 l6 3
32-81 l3 3l I I
Final Adult Mortality IZ 7
U U
~
~
-F*
~IT I
Concentration:
I
% Mortality:
l 1
MeanOffspring/Female:
Ls3.z
% Reduction from Control.l 3.8ye
--I;-
4
'V.-
1t A.
Environmental Testing Solutions, LLC Page 4 of 7 Species: Ceriodaphnia dubia Client: Sepuovah Nuclear Plant Date:
Ot-G5'-Cr3 coNc: 72%
Survival and Reproduction Data Replicate number Da 1
2 3
4 S
6 7
3 9
10 1
Young produced J
fl )
j) (O C) l Q
G)
Adult mortallty
.L L
L L
2 Young produced C
Adult mortality l
1 u
L 3
Young produced
[
l7lC-Adult mortality A
A UL 1
l l
I__
it-4 Young produced I S l Ll l rw4 Adult mortallty IL I
I L-I L-L L 1
I s
I Youngproduced l il C) [ (.C LO l
.... 4 M 4 C) [
l Adult mortality J
l 6s Young produced
\\7 l
_\\
1 z AdultmorItaty l
l T
TI Young produced V
l iJ i1 i
11 i 1& l i
Total young produced l
3l8 31 B6 2"q a
s l
- 1.
l3 13 Final Adult Mortality I
L I
ILr l_
L____
Concentration:
I
% Mortality:
O7 1
lMean Offitring/Fernale l %1
%-Reduction from Control:
CONC: 100%
Survival and Reproduction Data I_____ IReplicate number I
Day -
1 2
1 3
1 4
S 1
6 7
l 9
10 I
u-p I
M A u lt i o ~ 1
~ t _ L I_
1.4 1txungproduced Q) o k2 l
j)l(
ll
)
l l
U) l t) lO l
2 young produced Adult mortality 3
Young produced I
l O!
C 1 O.
Adultmortality
__ t!
i-I. '-
'-1 3
l Young produced Li S
§ W
l 1
l I
l Adult mortality t-j It Ll l _l l l
l 4
l Young produced l L
)(
1 12.
1 1
I I 1 O..L i Ito j ILA Adult mortality jL*_-
\\-,
I I
I L-I %l_
L-6 j
Young produced j(--,j I r)Lj\\
1
'1 I
l Adultmortality l T I L-l i l U
l i-l 1 l
l
^7)Young produced II Totalyoung produced 3
_J 33 Final Adult Mortality
\\-
- U.N'.'j 014-Concentration:.
l.
%Mortalita :
Om 7;
l Mean OffspnlngiF:male: -l 35.Z
% Reduction from Control: - lo.07.a
I I..
1
4 1
Environmental Testing Solutions, LLC PageS of 7 Species: Ceriodaplhnia dubia Client: Sequovah Nuclear Plant Date:
OR - 6OS 03 CONTROL - 2.
Survival and Reproduction Data Replcate number Day 2
3 4
5 6
7 1
9 10 I
l Young produced 0
D Q L o
I i2I..
o I Adult mortality L
2 Young produced O
O 1
CO 9
O 0
.Adult mortallty 1
I_
I 3
Young produced _
O 0
0O
[ Adult mortality I
L I I
t_
_I_
5_
4 Young produced l
1 3
3 S_ II I
Adualt mortaity l
> ~
S
=
Youngproduced 151 C2 101 L 10 IC l
L I 1t 1 Adult mortality l-U l--
LL I 6
Youngproduced I
O l
I Adult mortality
_ l v
l-l '-'
l l
t l-7 l
Youngproduced 1 i-Iii 115 IS 31 1J1f 16
%To 3
1 j
2.
Totalyoung produced 1
3 l
3S2. T z l 3, l l 3Z..
32T 3 l Final Adult Mortality l
_ l t
l l
l Concentration:
I
% Mortality:
T 1 Mean Offspring/Female:
ls32.0 l
% Reduction from Control: I k
CONC: 100% Intake Survival and Reproduction Data I I Replicate number l
Day 39I 1
2lo ng pr5duced
(
) l 0
i 1__
Ia_
I_
l lYtoungplroauced l o~
O l O j O j O
- X0 C7 O
c)
L
) £ L O mortality L
1 7 = _
I U1 _ L~
\\G I
L l2 Ydung produced jq iS jt i+
I mortality 3
Young produced
__i A
Adult mortality I
6 Young produced l_
I l
J Adult mortality l
L LZ U.
l l
l_
l Young produced j 1i1ll l Final Adult mlortality lLlll ll l
Adu-m-ta-
=
Total young produced 2A
-I 6 I L4 I 3z.
- 5.
Bo IaoI 2-Final Adult Mtortlty-!L.Z-T~
l Concentration:
e l % Mortality:
-l6^
lMean Offspring/Femnale:
L,1 I % Reduction from Control-. I A. !M I
Environmental Testing Solutions, Inc.
Verification of Ceriodaphnia Reproduction Totals Control-I Day ItR Uicate number Total 1 2 3
5 16 7
8 9~, -10 1 0 0
0 0
0 0
0 0 i 0 0
0 2
0 0
0 0
0
(
0 3 000 00000~~~
~~~00 0
-. 2
-S
.4 5
3 66 4
4 5
4 5t6 41 5 0 0
0 ~~~13 1 1 0
0 0
12, 15 51 6
12 12 1I 0
12113 I 0 7
7 16 15 15s1 1
15 1s 15
- 1. -
13 15 Total 33 30 3
3630 31 36 29 28 34 32 Control-2 Day
~~~~~Replicate numberToa Dy 1 2 13 14. 5 6
7 3
9 10 Tta 1
0 0
0 0
0 0
0 0
0 0
2 0
0 0
0 000 00 0
0 3
0 0
0 0
0 00 0
0 0
0 4
4 3
5 3
5 5
6 5
5 5
46 5
14 15 12 0
1-3 0 10 11 10 13 93 6
0 0
0 10 014 0
0 0 0 24 7
15 17 15 13 14 17 16 16 17 12 152
~~~~~~~~~~~~~- Total 33 353 632 36 32 32 32 307 2
10.98%
Replcate number Day -r2 3j 1 4~ -j-617 8~
9jj 1-Total I
0 0
0 0
0 0
O 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0000 0
4 6
6 5
6 4
4 3
6 55 s0 S
14 0
1 1
13 13 14 14 13 1
1 12 115 6
0 13 0
0 1
0 0
0 0
0 14 7
16 16 1
5 12 12 1
6 IS 13 17 1
5 ISO
-Total 3
35 3 31 30 34 35
- 32.
33 32 329
"
- 22%
Day R~~~Ieplicate number Total Day 5
2 3
4 S
~617 8
9 10 1
0 0
0 0
0 0
O 0
0 0
0 2
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
6 4
5 5
5 65 5
6 6
53 5 0 0
0 12 14 0
15 13 0
13 67 6i-1 12 12 0 1 0 1 3 0
0 1 1 0
61 7
16 17 a 161 1 3
14 1 X 1-7 1 5 17 161 Total 35 33 3
-33 [3-2 33 38 35 336 342 43.9%
Day I~~~~R Icote numberToa Da 2i 1314
-. 1--
17 8 I ' 9TotaI I
0 0
00 0
10 10 0 0
0 0
3 0
0 0
0 0
0 0
0 0
_0 0
4 4
6 6
6 5
5 5
5 6
5 53 5
0 0
013 12 15 12 12 12 14 90 6
13 13 1 1 0
0 0
0 0
0 37 7
12 17 16 16 15 17 14 14 14 17 152 Total 29 36 33 353 37 131 31 32 36 332 72%
Day
~~~
Renh~~It ute number oa Day 12 13 4.56 7
a 9
10 oa 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 3
00 0
0, 0
0 0
0 0
0 0
4 S
6 5
5 S.S 4
4 6
4 6
50 5
113 13 0
- 0.
0 12 14 0
14 16 2
6 1
0 12 14 13 0
0r 12 0
0 52 7
14 IS 17 13 13 13 17 S13 17 163 Total 33 37 34 37 36129 35 3
13 4
100%
I_
Day Re cote nuniber Total
-~~~~
2ii 3 4.Z S 7 8 9 10 I
0 0
0 0
0*
0 0
0 0
0 0.
2 0
0 0
0 00 0
0 0
0 0
3 0
0 0
- 0.
0 0
0 0
0 0
0 4
5 5-6 4
6 4
4 5
5 5
49 5
14 1412 0.13 0
11_16113 14 107 6
0 0
0 I.
14 3
0 1
01 30
'7 1716136 17 19 14 15 17 17 166 rIotal 36 3
63 37 37 32 36 3
3 352 100% IntakeRe Day Rpicate number.
Total 2
0 0
0 0
0 0
0 0
0 0
0 3
0 0
00 0
0 0
0 0
0 0
4 453 4
4 4533 3
5 5
41 5
141 13 0 I13 1II 14 12 0
0 10 ~ 87 6
0 01 13.10 0
0 0
111 0
35.
7 13 16 16 17 IS IS 17 16 14 7 156 Total 31 341 33. I'34.
31-32 I 32-30 3
3 1
Environmental Testing Solutions, Inc.
Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated client:
rest dates:
August 5-12,2003
'roject number: 791 Revelwed by:
Concentration Replicate number l
Survival Average reprodutilon Coefficient of Peruc. reduction from
(%)
1 2
3 4
5 6
7 a
9 10
(%)
(offspring/female) varao (%)
pooled controls (%)
Control -1 33 30 33 36 30 31 36 29 28 34 100 32.0 8.8 Not applicable Control - 2 33 35 32 26 32 36 32 32 32 30 100 32.0 8.5 Not applicable Pooled Controls 100 32.0 8.4 Not applicable 10.98%
36 35 31 31 30 34 35 32 33 32 100 32.9 6.2
-2.8 22%
35 33 35 33 32 33 38 35 32 36 100 34.2 5.6
-6.9 43.9%
29 36 33 35 32 37 31 31 32 36 100 33.2 8.0
-3.8 72%
33 37 34 37 36 29 35 36 31 39 100 34.7 8.7
-8.4 100%
36 35 36 31 37 37 32 36 36 36 100 35.2 5.8
-10.0 100% Intake 31 34 33 34 31 32 32 30 30 32 100 31.9 4.5 03 I~~~
I.0 3
)utfall 101:
ounnett's MSD value:
MSD:
itake:
'unnett's MSD value:
MSD:
2.512 7.9 1.684 5.3 MSD =
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction byI0.2% from the control.
Lower PMSD bound determined by USEPA (IH1 percentile) - 11%.
Upper PMSD bound determined by USEPA (9dh percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories foCerlodaphnia reproduction in chronic rccrcncc toxicant tests.
SEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-0003. US Environmental Protection gency, Cincinnati, OH.
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphola Survival and Reproduction Test.Rtpreduetion Start Date:
IS103 Test ID:
CdFRCR Sample ID:
TVA. Sequoyah Nucler Plant Outfall 101 End Date:
V12103 Lab ID LTS-Env. Testing Solutions Sample TypO:
DZMR4)ischge Mouioing.epoet Sample Date:
hotocol CHRONIC(PA421-R.02.013)
TestSpecies:
CD-Carodsplhiadubia Commmtr Comparison to Control-1.
Cae9.%
1 2
3 A
5 6
7 5
10 Coneul-l 33.000 30.000 33.000 36.000 30.000 31000 36.000 29.000 2t.000 34.000 10.91 36.000 35.000 31.000 31.000 30.000 34.000 35.000 32.000 33.000 32.000 22 35.000 33.000 35.000 33.000 32.000 33.000 3S.000 35.000 32.000 36.000 43.9 29.000 36.000 33.000 35.000 32.000 37.000 31.000 31.000 32.000 36.000 72 33.000 37.000 34.000 37.000 36.000 29.000 35.000 36.000 31.000 39.000 100 36.000 35.000 36.000 31.000 37.000 37.000 32.000 36.000 36.000 36.000 Trusfosr Uatransrrmed Rank 1-Tailed
- Iseteic, Ceae-%
Meta N-Mess Mean Mle Mat CV%
K Sum Critical Meas N-Mes Conntzo-l 32.000 1.0000 32.000 21.000 36.000 L839 10 33.700 1.0000 10.98 32.900 1.02t1 32.900 30.000 36.000 6.155 10 115.50 75.00 33.700 1.0000 22 34.200 1.06S3 34.200 32.000 3S.000 5.650 10 126.00 75.00 33.700 1.0000 43.9 33.200 1.0375 33.200 29.000 37.000 1.007 10 117.50 75.00 33.700 1.0C00 72 34.700 1.0844 34.700 29.000 39.000 8.704 10 130.50 75.00 33.700 L.00D0 100 35.200 1.1000 35.200 31.000 37.000 5.807 10 137.50 75.00 33.700 1.0000 Auxi Teats Statistic Criical Skew Kurt Kolmogorov D Test idicates non-normal disttibhtion (p - 0.01) 1.04534006 1.035
-0.194412
.0.5727649 Buddrs Test isicates eqiual varmacee (p -0.66) 3.27994871 15.0863171 Tbe contrl means we not ipificzatly diffavat (p -1.00) 0 2.10092367 Hyptheis Test -tilOS)
NOEC LOEC CkV TU Steers Many-One iank Test 100
>100 I
Treatcmab Ys Cont1ol-i Linear laterpolatie (200 Rsmpleis)
Paint e
SD 95% CL Skew 1005
>100 MC1O
>100 IC15
>100 1.0 1C20
>100 0.9 IC25
>100 0.8 IC40
>100 0.
IC50
>100 OJ 0.6 I O.5
=g 0.4 KC.0.2 0.1 0.0
.0.2 0
20 40 60 go 100 120 Dose %
Dea-IRespease Plot 4S 40 35 "25
,20 15 10 o...5 0
9 A~~~~~~~~~~~~~~~~~~~~~~~~~~~~
zqnP-05-03data a-r
Environmental Testing Solutions, Inc.
Statistical Analyses Used for PMSD calculation only.
Cerlodaphula Survival and Reproduction Test-Reprodocdon Sart Date:
8/12103 Test ID:
CdFRCR.
Sample ID:
TVA, Sequoyah Nuclear Plant -Outfall 101 End Date:
8/19/03 Lab ID:
ETS-Env. Testing Solutiwis Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol:
CHRONIC-(EPA-821-R.02-013)
Teat Species:
CD-Ceuiodaphnia dubia Ccnments: Comparison to Cootrol-1.
Cone-%
1 2
3 4
5 6
7 8
9 10 D-Control 33.000 30.000 33.000 36.000 30.000 31.000 36.000 29.000 28.000 34.000 10.98 36.000 35.000 31.000 31.000 30.000 34.000 35.000 32000 33.000 32000 22 35.000 33.000 35.000 33.000 3Z000 33.000 38.000 35.000 326000 36.000 43.9 29.000 36.000 33.000 35.000 32000 37.000 31.000 31.000 32000 36.000 72 33.000 37.000 34.000 37.000 36.000 29.000 35.000 36.000 31.000
. 39.000 100 36.000 35.000 36.000 31.000 37.000 37.000 32000 36.000 36.000 36.000 Transform: Untransformed 1-Tailed Conc-.%
Mean N-Mean Mean Mb Max CV%
N t-stat Critical MSD D-Control 32000 1.0000 32000 28.000 36.000 8.839 10 10.98 32900 1.0281 32900 30.000 36.000 6.155 10
-0.819 2.287 512 22 34.200 1.068S 34.200 32000 38.000 5.650 10
-2003 2.287 2.512 43.9 33.200 1.0375 33.200 29.000 37.000 8.007 10
-1.092 2.287 2512 72 34.700 1.0S44 34.700 29.000 39.000
. 8.704 10
-2458 2.287 2512 100 35.200 1.1000 35.200 31.000 37.000 5.807 10
-2913 2.287 2.512 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-nornnal distribution(p <-0.01) 1.04534006 1.035
-0.194412
-0.57276493 Bartletts Test indicates equal variances (p - 0.66) 3.27994871-15.0863171 Hypothesis Test (14ail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 100
>100 1
2.51186626 0.07849582 14.56 6.03333333 0.0478387 5,54 Treatments vs D-Control
.tqnfi8-OS-03dataJ.xls
Environmental Testing Solutions, Inc.
Statistical Analyses Ceriodaphnis Survival ad Reproduction Test-Reproduction Start Date:
812W3 Teat ID.
CdFCR Sample X TVA. Seqnoyah Nuclear Plant -Intake EcdDatc:
8/19/03 Lab ID, ETS-Env. Tasting Solutions Sample Type:
D)MRDischargeMbnitoringRzport Ssnple Datc:
Potocol:
CHRONIC-(PA-821-R-02.013)
Test Species CD-Cediodspaimi dubia Comments Comparison to Contrnl-2.
Coue-%
1 2
3 4
5 9 7
3 9
10 D-Centrol 33.000 35.000 32.000 26.000 32.000 36.000 32000 32.000 32000 30.000 100 31.000 34.000 33.000 34.000 31.000 3Z000 32.000 30.000 30.000 32.000 TrIansform Untransformed 1-Tailed Isoteanle Coce-%
Mesn N-Mean Mea Min Max CV%
N t.Stat Critical MSD Mean N-Mema D-Coitrol 32000 1.0000 32.000 26.000 36.000 5.463 10 32.000 1.0000 100
.31.900 0.9969 31.900 30.000 34.000 4.543 10 0.103 1.734 1.6S4 31.900 0.9969 AuxiliaryTests Statistic Critical Skew Kurt Shapiro-WiLk's Test indicates onnal distribuion (p > 0.01) 0.9145423S 0.S68
-0.7S8259S 2.67552132 F-Test indicates equal variances (p - 0.0) 3.49206352 6.54108572 Hypothesis Test (1-tail, 0.05)
MSDu MSDp MSB MSE F-Prob df Hmnoscedastic t Test indicates no significant differences 1.6S421476 0.05263171 0.05 4.71666667 0.91913337 1,1S Trantments vs D-Control LInear Interpolation (200 Resamples)
Point SD 95% CL Skew IC05
>100 ICI0
>100 ICIS
>100 1.0 IC20
>100 0.9 IC2S
>100 IC40
>100 0.8 ICSO
>100 0.7 -
- 0.6 0.4 0.3 0.2 0.1
)0.0 0
20 40 S0 80 100 120 Dose %
Dose-Response Plot 40 35 14ail, 0.05 level 30
- of significance
-c 2 o25 0
10 8
sqn_08-05-03datal.s
Environmental Testing Solutions, LLC Page I of 6 I.
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'4' 1.
Eniomna Tetin So'ios LL ae Chronic Whole Efmluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: TVA Facility: Seauorah Nuclear Plant UV-Treated NPDES #: TN 0026450 Project #g::..-I
(
Dilution re information:
Comments:
Diluton prep
.')
I O
__L
_q__
Effluet.yotumc (mL)
Z14.bfu0
-- IR IqM o
Dfvum
)
.v lSm n
11-1 sco 0
Total Volume (mL) 7JO 7CC Z=
=
zo Test organism informatio r.'
7s.5 Test information:
Organism age:
7 F0 Lba Randomizing template:
i Date and times organisms Incubator number:
were born between:
1300 1 s30 Organism source:
AMS 6 Wof0l-C4 Artemia lot number:
0 l
Transfer bowl information:
pH -
Temperature =
°C Total drying time:
- 1. e Z6l Co Date / Time in: of, 12 R3IoO Average transfer volume:
Date I Time out: O1*a Q
I Oven tem perature:
101_C
. I.......
Dailyfeeding and renewal information:
Day
.DatE-Morning Afternoon Test initiation, Control water Sample numbers Analyst A, k
- -fe~eing feeding renewal, or batch used used
_ time time termination time K14$
o-ce-a "e :
iSsa LL4 ZZ a____-0____
2___
Mo MOO CS 1
a46 a
fm oa 1cObl.65 A
dOt < f 3
-eR_
__o_
Z-C 0 tU I,Ol.
6t oR-as OR s tco 835S ObM u5
¢0 tal.061 -L ozA 5 OV-lt-03 0P~
ISos IlAC:
08-e-k'f
- lt0-6 rwss 135R A
7 1 k. Iz'OS 1 l5
- la &
Control information:
Acceptnce criteai Summary of t t endpoints:
% Mortality:
-'4
_20%
7-day LCso 7 iet, Average weight per initial larvae:
O. '0Cc 5________II_
NOEC._
_I Average weight per surviving larvae:
C.'oNe 2 0.25 mg/lare LOEC
> 1 7.
ChV IC25
',1 > i6dZ
Environmental Testing Solutions, LLC Page 2 of 6 Environznen tal Testing Solutions, LLC Page 2 of 6 a
I 2
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-"-'I
-'I 1
4,',
Species: Pimephales promelas Client: TVA I Sequoyah Nuclear Plant UV-Treated Date:
OR-OS C3
- __ __ ___ __ __ ___ _Survival and Growth Data
. Day CONTROL 10.98%
22%
A B
C D
E F
G H
I
.J K
L 0f.
°__.=_.
10 1010
.10 i-
/0 /0
.0 to
/-/0 I&
1 10 tO _
-0 I)
_o
/)
I O)lI) /0 10 2
/0
/0/
/0
/0 /0
/t 0/0
/0 /6 0( to 3
/__
/0 lo 1 1/O /6 0 I
_--tO
/
10 4_
/0
/O
/0
( 0/o/
/6
/ 0t '1 0 / 0 /
6./0
{)
a/0/0D
/
/0 /0 o/o to I
/
6, 7__
/0
/0
/0
/0(
6
/
/0/0 Q 0 /0 /0 1O A-Pan weght (mg)
Larvae weight.(thg) w.s-B dt t
4_
_ ~~~..
Ot Ok.
v I*
IP 0~'
Akv Weight per Initial number of O*
eC I Initil number oflarvae j
t a
8 0
° v.
1Y'
.Average weight per kintal1..
nunber of larvae (m.g)
I,0 o Fq l Calculations and data reviewed:
Page 3 of6 Species: Pimep hales promelas
'Client: TVA / Sequoyah Nuclear Plant U V-Treated Date:
OUC6-0a~
Sumbial and Grawf A Data Da~y 43.9 9%
72%
100%
M N
0 P
Q R
S T
U V
W X
/0/0 1010
/0~/6 lb l/b/00
/6 6/10
/1~~~
,~~,O g
101 /O IL-)
lX b/Ik)
J 2
/(
1
()
f I C
oA 10 /0 /0
/0/
1/010
/0101610/OIL)~~~~~~~/
/0
/0/
6 /0/6(
_______~~~~I 110 l0(to10 10 it/O/I0./O
)O 6
lolL)(
1
/0/0/
/O i0,it)
/0/01 1 //6(01 10
/0(0(0f
/vKto 0'
A - Pani weight (mg)
~__
B-Pan, Marva weqht (mg)
Larvae weight (rng)'.-A -B
\\4 Wegtper Unital number of Weight 4A~~~~~~~~el4,O P
-Clhntialnurnmberoflarvae vd
\\
6 V.V
\\
Average weight per Initial number oflarvae (mg)
.1.
Calculations and data reviewed: 6 Comments:
Environmental Testing Solutions, LLC Page 4 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant UV-Treated Date:
Of-
-63M A-P t
-s B-P
'~~~~~~~~'
15
~~~~~~~~~~~larva
-C' Aver numi Comments:
Survival and Gro.t6 Data Day 100% Intake Y
Z AA BB 1
/01 0 1/0 ic 2
_t/
16
/0 (0
4_
1 0 (
/ 0/() 6t i /
10 10 7s It ) I C )1 0 / 6 /
6 i c ~ ~
1 Q 1 0 1 0 7
10(0~/O10 1(
an weight (mg) an + Larvae weight (mg) re weight (mg) - A - B hti per Initial number of lidtial number otlrvae
\\
8 '
age welght per Initial er orlarvae (mg)
- l.
(eO 2.
Calculations and data reviewyed:
I
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Vcrification Dnta Entry, Calculations, and Statistical Analyses.
Client:
Tet dates:
TVA Sequoysh Nuclear Plant. UY Treat Me August 5-12. 2003
- C Projed number.
791 PeVhd br Cearadaa ()
Replate taitids ibnr a rFlan mbe, drian.
A -d-Pa.wigha-5 - Pa. t+La Larvsa welght C t-t igt I illidaS asmmber* Me.
Me tsrt Mnrd a weiht Cms) Cnlasattarlaa Perael rowtte sfr lva
- loit (we
-A-.
,.a n (lo) tt))
tit t)
A 10 10 14.735 24.020 9.285 0.9285 Control R
10 10 14.748 23.380 9.132 0.9132 100.0 0.9063 5.0 Not applicable C
- 0
- 0 15.011 24.440 9.429 0.9429 D
10 10 15.105 23.510 3.40$
0.t405 E
10 10 15.1S0 26.0S0 I0.900 1.0900 le.98%
F 10 30 14.945 26.200 11.255
.1255
- 00.6 1.0653 s.0
.17.6 C
10 10 15.131 25.560 10.429 1.0429 H
10 10 15.041 25.090 10.049 1,0049 I
30 10 14,707 24.080 9.373 0.9373 22%
J 10 10 15.263 25.690 10.427 1.0427 K
10 10 14.653 24.370 9.717 0.9717 L
30 10 15.200 25.190 9.990 0.9990 M
10 10 14.831 24.940 10.109 1.0109 4319%
N 1
0 30 14.726 25.520 10.794 1.0794 100.0 1.0336 10.2
.14.1 0
3 0 10 14.999 23.990 t.991 0.t991 P
30 3 0 14.719 26.170 11.451 131451 10 10 14.959 27.230 12.271 1.2271 72%
R 10 30 15.063 27.020 11.957 1.1957 300.0 1.1211 9.2
-23.9 S
10 10 15.020 25.410 10.390 1.0390 T
10 30 14.9'5 25.300 10.305 1.0305 U
10 30 15.012 25.910 10,398 1.0391 100%
V 030 5.009 26.220 11.211 1.1211 W
10 10 15.109 27.770 12.661 1.2661 X
10 10 14.759 24.940 10.111 1.013t Y
10 30 14.371 26.710 11.909 1.1909 300% Intake Z
10 10 15.037 24.370 9.233 0.9283 100 e.0602 10.4
-17.0 AA 10 10 15.1t4 26.010 10.916 1.0916 BB 10 10 14.300 25.100 10.300 1.0300 Outibll 1ol:
Ounnet's MSI) value:
PMSD:
Intake:
Dunnet's MSD valuw.
PMSD:
MSD -
Mhinium Signifiant Difference 0.1380 15.2 0.1158 12.3 PMS1D P ercent Minimum Significant Difference PMSD ha m*easure of test precision. The PMSD is the mninimumn percent difference between tOm control and treatment that can be declared statistically aignificant in a whole efuent toxicity tat, On r e, a siniicant Micn occun for Enonunenta Testing Solutions, Inc chronic toxicity tests when a toxicant reduces Pimepholca growth by 1607% fion dte control (determined through reference toxican testing).
Lower PMSD bound deternined by USEPA (10th percentile) - 9a4m Upper PMSD bound determined by USEPA (90th percentile) - 359.
The lower and upper bounds were calculated by the USEPA uing 205 teats conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Varinability in Whoic Efuent Toxicity Applications Under the National Pollutant Discharge Elmindation.Program. EPA-8331R-004003. US Environmental Protection Agency, 1"..:..
4 1f; EalI
Environmental Testing Solutions, Inc.
Statistical Analyses I
Larva FIsh C.*% b and 3arnvt'al Tert-7 Day Growths Staft Date:
81513 Test a.
PpFRCR Saznpi 1D TVA.Sequayah Nuclear Plant, Outfall 10t End Date:
811210 Lab 10.
FETSBW. Testng Sauow s "saml Type:
DIAR-Dischune Moniatar Ralept Sample Daze Prolocat CHRIONJIC.(EPA42.1-R42.013)
Tet species:
PP-Piznephales, promelas Cosmenits:
UY4reat4d.
Conc.%
8 2
3 4
D-Cantro 0.923 0.9132 0.9429 0.3405 10.98 1.0900 1.125 1.0429 1.0049 22 0.9373 1.0427 0.9717 0.999 43.9 1.0109 1.0794 0.5991 1.1451 72 1.2271 1.1957 1.0390 1.0305 too 1.089 1.1211 1.2661.
1.0131 TuaasfOrm: Untransearood 1-Talled isotoglc COR-%
Mean 14-Mean m1esa Min Max Cy%
14
-Stat Critical MsD Mean K.14Mean l).Csssk 0.9063 1.0000 0.9063 0.3405 0.9429 5.020 4
1.0400 1.0000 10.98 1.0658 1.1761 1.0658 1.0049 1.1255 4.960 4
.2.787 2.410 0.1380 1.0400 1.0000 22 0.9877 1.089 0.9877 0.9373 1.0427 4.509 4
.1.422 2.410 0.1380 1.0400 1.0000 43.9 1.0336 1.1405 1.0336 0.591 1.1451 10.168 4
.2.224 2.410 0.1380 1.0400 1.0000 72 1.1231 1.2392 1.1231 1.0305 1.2271 9.158 4
-3.787 2.410 0.1330 1.0400 1.0000 100 1.1233 1.2400 1.1238 1.0131 1.2661 9.274 -
-.3.799-2410 0.1380 1.040
-1.0000 Auxillar Tests Statistle Critical Skew Kurt Shsapiro-Willes Teat itdicates normal distiitidon (p 2, 0.01) 0.9894892 0.884 0.08004414
.0.3285299 Dearletfs Teal indicates equal vnarinces Mp048) d,30414085 15.0863171 llypothesls Test (W.all. 0.0S) 140CC LOEC OWV TU MAS~a MSOP MSB MSE F-Prob 4?
Dtauleenas Te 100
>l00 I
0.13797485 0.15224391 0.02819804 0.00655535 0.00947229
- 5. 18 Treaftnents vs B-Contral LUneay Interp.Iatioo (2N0 Ressanples) point SD) 911% CL(Exp) skew I=I
>100 MCIS
~
01.0 ICZO
~ ~ l>0 0.9 IC25 21l00 0.8 1C40 3400 0.7.
> l00 0.8 0.5
.4, I0.3 C0.2 0.0 40.2!
- he~~~~
~~~
~~~
~~~~~~~~
20 40 80 so10 2
~~~~~~~~~~~~~~~~~~Doze Dose-Rexponse Plot 1.2 O'll I-t~~~~~~~~~~~~~~~~~ail.
0.05 level of significance C.6 0.4
&qn_08-O5-0Jdafao.xfx
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Stat Date:
35103 Test ID PpFRCPL Sample ID TVA-.Scquyah Nuclear Plant. Intake End Date:
V12103 Lab ID:
STS-Easv. Testing Solutions Sample Type:
DM3R-harge M.torming Reot Sample Deae Protocol:
CHRONIC.(EPA4J2-R-02413)
Test Species:
PP-Pimephales promelas Comments: UVixested.
Conc-%
I
. 2 3
4 DOcoctrol
-Q92S5 9132 0.9429 aua5 100 1.1909 0.9283 1.0916 1.0300 Tranbnrm: UntanasrormM d-Talled Cenc-%
Mean N-Mean Mea Min Mal CV%
N 8-Stat Critical MSD D-Cor=[
0.9063 1.0000 0.9063 QS8405 0.9429 5.020 4
100 1.0602 1.169S 1.0602 0.9213 1.1909 10387 4
- 2.5S4 1.943 0.1158 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-WillksTest indicates nomal distrition (p 0.01) 0.9668S405 0.749
- 0.11350151 0.31562764 F-Test indicates equal variances (p - 0 1 )
5.15831976 47.4683456 Hypothesis Test (1-all, 0.05)
MSDu NISDop MSB hSE F-Prob dr HomnocedastietTesindicatesnosilnificantdilfetences 0.11576171 0.12773354 0.04738581 0.00709796 0.04155713 1.6 Tmatments vs D.Control Dose-Respnse Plot t4,
- I 4 10.8 a
I.
0.4 0.2 a
I
.so...
I 1-tail. 0.05 level of significance I
sqrn08.OS-03data.xfs
lEnvironmental 'v'csting Solutions, LLC Chronic Wholc £IMient Toxicity Test (EPA-821-R-02-013, Method 1000,0)
Species: Pinelphlespromelds i
Davily Chemical Annlyscs Sequoa.rh Nule~ar Pliant Non-Treated I
I.
I, Rleviewed by.-
4eL I est dates:
August 05. 12, 2003 briject nunmber:
791
.7-7,..
Concentration l 1aranacter iay I r
- IAY I I
Iay 2 I
Day 3 4
4-p11 (S(J) 1)0 (mg/l.).
Conductivity (uimhos/crn) t~~~na~l uhital IFInal Initial 7.581 7.681 7.52 7.62 7.61 60 3_
3S l
1 84 1
24.81 25.3 24.71 25.4 V r6 a1 Inilhal I ImIu 8.9
.U4 7.8-7;k~~~~~~~~~~1 It 7
Control I~~4 Alkailinity (mgtl. CaCO) z I
llardness (mzf. Cua(l) 3)
Temperature (C) 4-I 25.11 24.91 25.51 24.N 25.4
- 1.
24.9 25.41
- 24.91 25.31 25.6 P"i (81U) 7.5X 7.41 7.56 7.41 7.38 7.52 7.67 7.48 7.58 7.37 7.66 7.84 8.01 7.X7 1)0 (mg/l) 7.6 7.3 7.8 7.4 7.7 7.8 8.2 7.5 8.1
. 7.2 7.7 7.5 7.8 7.5 Condutivity ( imhoscm2X) 298
_ 309 temperature (MC) 25.1 24.9 25.5 24.S 25.4 24.8 25.3 24.7 25.4 24.9 25.4 24.9 25.3 25.6 pil (.SU) 7.56 7.43 7.56 7.41 7.37 7.53 7.67 7.47 7.57 734 7.65 7.84 8.01 7.86 22%
i)O (mg/l )
7.7 7.3 7.8 7.4 7.6 7.7 8.2 7.4 8.0 7.0 7.7 7.4 7.8 7.5 2Conductivity (;.7imos/m) 294 6
4 295 I mperature CC-)
25.2 24.9 25.5 24.8 25.4 24.8 25.3 24.7 25.4 24.9 25.4 24.9 25.3 25.61 pI uSM) 7.521 7.3N1 7.531 7.401
'7.311 7.521 7.651 7.471 -
- 7.541, -
7.31 7.601 7.82 8.001 7.83 43.9%
1)0 (ingil.)
7.7 7.3 7.8 7.41 7.6 7.7J 8.2 7.6 1
8.0. :
- 7.
7.07 77.4 7.8 7.5 Conductivity (jimn
- 61)
I26 261 5
- 1.
256; 260 255 266 rncmnerature 1o(()
25.21 24.'
25.51 24.8 25.41 24.8 25.3 24.71 I 25.41 24.91.
25.41 24.9 25.31.
25.6 4
4
a nil I5SIJ 7.4)1 7.35 7.491 7.41 7.25 7.52 7.62 7-461 7.481 7.31 7-531 7.85 7.T91 7.81 72%
1)0(mg/l.)
i7 7.21 7
4 7.61 71 8-2 8i
.6 80 7.0 i.e7
.0 7.4 L: tduct vity lm2oln)
222 218 227 Temperature CO 25.4 24.8 25.31 24.71 1
- 4.
4.
nil (14U-l 7.39 7.16 7.5.4 7.53 7.43 DX) (a11u1.)
Conductivity (unaho.s-a)n.
iQO%'.
Alkalinity (rngL CaCO3)
Ila nces (mgIl CWCY)I 7.21 7.8Y 7.41 7.8
.i.7 X.2 7.61 184 I
NO s180
~~~~~~~65 Ill 4.9 25.5 248.
25.4 24.R 25.3 24.7 f
I;otal Residual (.tlrn (m/l 0
remperature (C) 25.4 i !Vi:4 24.91l:
25.41 24.91 25.31 25.1 nil IthS. --> -.
_~-.--
1)0. tlll L.)
Conductivity (pmthos/em) 100% Intake Alkalinity (mgtl. CUC0M) 7.461 7.351 7.421 7.361 7.441 7.541 7.581
- 7.471.
7.44k.i s 7.35j
-- 7.441 7.8 7.8 7.2 7.7 7.X 8.2 7
5 i
I 8..
7 1 ;
8.0.-
61
_1
_E6
__.,--62 1 25.1 2.S 1
25.51 24.81 2-5.4
-24.8
.25.3
- 24.
1 25.4 1-;
24.9 25.4 2
7.X[
At9t7L 7.90 Iiurdnems (mt!l. (7aC('O)
TI'otai Resilduial (C.lorine (mg/lidj)
Temperature ('*C)
Environmental Testine Solutions, LLC
-Page 5 of 6 EnirnenalTstngSluios LC ae o Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant Nontreated
- Daily Chemistry:
Date: OC-M5-CS3 Day Concentration Parameter DaY CONTROL pH(S.U.)
2 ise
- I3 DO (mglL) 73 Conductivity 21
.I
...... __Alkalinity.._
3,o.
AB~(M CaCO31L) "_-. c_
Hardness (mg CaCO~L Teinperaturc(C)
()
25 3
2
'2
.6 pH(S.U.)
,5*
1, I.
4V 10.98%
DO (mglL) 1(o Conductivity zle (iunhoitcm).
S9
_0_
Temperature (TC)
Z5 zq.c 2;5 ztte pH (S.U.)
- 3
.4l Si 22%
DO (mg/L) i3ato Conductivity
.q (gmhoslcm)
Zq Temperature (Cc)
ZS.Z zq z4q6 Z"
L.
24.6 pH (S.U.)
2 i
3
- e3
,D
- .1j-S 43.9%
DO(mg/L)
.A.
Conductivity Z
51_
(Ounhoscm)
Temperature (MC)
%5
-L 2 *9 zSS ZS. ti l
6 pH (S.U.)
gjI' I
.4l I
I U
I II 72%
z,,.N DO (mglL)
I 11 iU.,
' I Conductivity Z 3 hstm I
i Iz.-
J Temperature (tC)
Z.5.4 I
t -
pH (S.IU.)
.r g
100%: :.
I Ot(m6gL).
Conductivity (ui'hos/cm) 1_
Alkalinity (mg CaCO9tL)
Hardness
-1l (mg CaCOz/L).
TR chlorine (mg/L) 40 410 Temperature (MC)
S. 3
.1 6*
~~~~~~~--
P.
pH (S.U.)
100% Intake DO (mg/L)
Conductivity (gmhos/cm) 63 Alkalinity l
(mg CaCO3/L) l Hardness l (C (mg CaCO,/L)
~
.1 Temperature (°C)
Initial I
Environmental Testing Solutions, LLC Species: Pimepthalespromelas.
Client: TVA I Sequoyah Nuclear Plant Nontreatedr Concen-tration Parameter 3
I 4
4
- 4. _.
Z CONTROL pH (S.U.)
-4.U19 I
I -qtoz 4-
.1-DO (mp/LI Conductivity 3I5 (umhoslcm)
Alkalinity (mg CaCO 3/L) m___
Hardness (mg CaCO3/L)
- f 1.640 1 -.
25SA
~
Page 6of 6 Date: cf-6 o-Q o07:
11A~~~~~(w FA A
~
5 861 1-42 ~
-*q1 4.Io g4A c~z Temuerature (C) zt I-t.
'I. ~- *
~- -
0 10.98%
DH (S.U.). -
DO (mg/l.)
.J
- .~~11 Conductivity (gzrhos/cm) 21 Temperature t°)
- W'4 I 2.4 4-
- 1]
6"
]
pH (S.U.)
1-t.-57
- 4.
a...Z
.41
4-22%
DO (mg/L)
I a fn I
Conductivity (umhoslcm) 1 1a6!
Temperature (C)
Z5.3
_=_
r pH (S.U.)
- 1. 4L Li-%.O
--I.
M a
zt9(,p Z'S.4 zq
-+,54
-4.
.6.0
-4 7-J,60 1
1 Z,7.4 zq I&U M 764 M i-Ti I
u I
vs. 3
?.S4.
I 43.9%
DO (mg/L)
V J
Conductivity (pmhos/cm) lazo I
- 1 16 1
L Z.55 1
Mf 7Ztefo Temperature (°C)
I
't 7z.L 14A.
1-.a I
pH (S.U.)
I
.53
-4q9
-4 1 I
72%
DO (mg/L)
Conductivity 1-I Tenfperati~re CO, -'
pH (S.U.)
.a -
100%
DO (mghIA S
Conductivity * * -
bumhos/cm)
_18__
Alkalinity (mg CaCO3/L)
Hardness (mg CaCO3/L) 1L0 6Io 2-'L~
-16
M OEM 244A
-F 2 e)
N Temperature (°C) 6*
4~~~~~~~~~~~~~~~~~~~~.~~
P pH (S.U.)
'-15'6 fl-+t9 I -4.'I' 100%
Intake DO (mg/L)
Conductivity (gmhos/cm)
IC Alkalinity (mg CaCO31L)\\
Hardness (mg CaCO3/L)
,..q.1-
,G.,
TemDerature AC)
-5 a
'5 t -~~~1,"
V Lqc -,I zg;. S 1 25-- f.
I finil. I initial. I Final I
Initial I
Final I
Initial I
Final I
Initial I
_ _. i a F i a i n t a Fn a l I
i nt_
F i n i
_a_
-l-
Environmental Testing Solutions, LLC Chronic Whole EllienT 'I'oxicity Test (EPA-82t-R-02-013, Met Species: Ceriodeaphiiie dRbin Daily Chemical Analyses hod 1002.0)
I Client:
Sequoyalh Nuclear Plant Noa-Treated
'rest dates:
August 05 - 12, 2003 I'rojcct number: 791 Cuoncentration Parameter DJ y U)
- jIa 2
DIay 3 Day 4 Ibay 5 Day 6 Imitial I1in JI uiliul lni Initial Finai Initial Final Initial Final Inital I Finai Intial Final Oil lSU) 7.82 7.5 7.63 7.68 7.491 7.63 7.68 7.61 7.62 7.50 7.391 7.97 8.04 7.94 DO (mg..)
7.8 7.8 7.9 7.6 S.0 7.6 7.6 S
7.91 7.7 7.5 7.7 (onductivity fjmhosem) 321 311 310 315 303 302 312 Control Alkalinity (mgI.
CaCO))
59 61
_60 lbrulness (mg!L CaC03) 86 as i
84 Tremperature CC) 25.1 25.0 25.5 25.1 25.3 25.3 25.4 25.1 25.5 25.0 25.3 25.2 25.2 25.2 PR1 (SUi) 7.58 7.57 7.56 7.72 7.38 7.63 7.67
_ 7.61 7.574 7.66 7.98 8.0!
7.92 DO (ILd) 7.6 7.6 7.8 7.9 77 8.0 3.2 8.0 8.1 7.6 7.7 7.6 7.87 IQ.9X% Conductivity (tmhostm) 298 29_
282 290 300 298 309
.remptersaturc CC) 25.1 25.0 25.5 25.1 25.3 25.3 25.4 25.1 25.5 25.0 253 25.2 25.2 25.2 pil (SU) 7.56 7.56 7.56 7.75 7.37 7.62 7.67 7.62 7.57 7.49)
T65 7.96 8.01 7.9 22%
I
)O (II I
FVL) 7.7 7.6 7.8 7.9 7.6 8.0 8.2 8.0 8.0 0
7.6 7.7 7.6 7.8 7.7 Conductivity (Iilios/cm) 29.4 287 276 285 286 284 295
_remperature CC) 25.1 25.0 25.5 25.1 25.3 25.3 25.4
- 25.
25.5 25.0 25.3 25.2 25.2 252 pil (SU) 7.52 7.55 7.531 771 7.31 7.61 7.65 761 7.54 7.51 7.60 7.97 8.00 793 1)0 (mgjl.)
7.7 7.5 78X 7.9 7.6 8.0 8.2 7.9 I
8.0 7.7 7.8 7.7 7.8 7.7 C.onductivity (Imisloskr) 266 26i 251 256.
260 255 266 Temperature (C) 25.2 25.0 25.5 25.1
- 25.
25.3 25.4 25.!
25.5 25.0 253 25.2 25.2 25.2 pit (SU) 7.49 7.54 7,49 7.8!
7.25 76!
7.62 764 7.48 7.51 7.53 7.99 7.93 7)DO (mg1L) 7.7 7.5 7.8 7.9 7.6 8
8.2 7.9 8.0
.7 7.8 7.7 7.8 7.7 72%
C:onductivity (jcmhuskm) 223 222 217 222 21 227 Temnperature (C) 25.2 25.0 25.5 25.1
- 25.
25.3 25.4 25.!
25.5 25.0 25.3 25.2 25.2 25.2 p1! (Sli) 7.47 7.54 7.43 7.16 7.601 7.58 7.69 7.45
.7.52 7.47 7.98 7.98 7.94 ID)tlm/l.)
7.7 7.51 7.8 X,0 7.X 8.C0 8.2 8.0 7.7 7.9 7.71 7.8 7.7 Conductivity (lindlos/em) 180 519 lI)Of.;
Alkalinity (mg/l. (ACO])
61 63 illrulness (mg/l. CaCO3) 74 74 4I ITotal Resitdual (Ctalline (t4g/lI.)
U.v10
_0.1 Temper turc( 9C) 25.2 25.01 25.5 25.1 25.3 25.3 25.4 25.1 25.5*
25.01 25.3 25.2 25.21 25.2 pit (SIJ) 7.46 7.54 7.42 7.52 7.44 7.62 7.58 7.6 7.44
.541 7.44 8.01 7.97 7.)5 DOt (Imug/L) 7.81 7.51 7.87.
7.71 8.01 8.2
.0 I 8.0ko w.78 3.01 7.8178 C(onductivity (jmuhos/em)
I X
3 0
174
__t28:
100% Intake Alkalinity (mg/l. CaCO3) 61
__3_.
n_
IlaruIne s(m/I (:aC(.
- 3) 76 472 IotaI R (ihirlir(mul.)
- 18 It)
<.l_
Temperature CC) 25.2 25.0 25.51 25.1 25.3 25.3 25.4 25.1 25.5 25.01 25.31 25.2 25.2 25.2 i i I
. I
lEnvironmental Testing Solutions, LLC Page 6 f7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant Date: -
R -oS- 07 Daily Chemisfry:
1 r
Concentration Day Parameter 0
I 1
I 2
I z
CONTROL pH (S.U.)
4.6 2 I 3.- 5:- I -q t
\\ _. _.,
\\
ff DO (mg/L)
Conductlivty 32(
(gmhos/cm) 3 Alkalinity '.s.d. -
(mg CaCO3/L)
_5___
Hardness lLo (mg CaCO3/L)
I
-4. ts 1
-6 ((
I
.1 V,
1z.
7i.31
- t440, Temperature (C) 7S-t Zs-I 9
I J-pH (S.U.)
1.56 4.5-1
-hz6 I :Bids - I
10.98%
A DO (mWL) 41.lo
'-4-J6
- 1A.-
V r
I I.
Conductivity 29 9Z (iimho /r) 1 I 8L1
~
6 Temperature (MC) 2Z5.
K.
pH (S.U.)
w-4-5
.I..-
22%
DO (m/L)
(w.iho /M)
I_____
Temperature (C) 75.1 Z75.
xS.3 zs.s
.1 K.
4 lb 4
4 pH (S.U.)
-. 5Z 4
0 43.9%
DO (rng/)
Conductivity (Limhoslcm) 2 (I o o 1* l
,It-to 251 Zs -23 8.0 mm=
?m -S Temperature (°C)
ZS. 7.
a
.a o}
l u
vH (S.U.)
-J. qq 4 -.e I
72%
DO (mFlL) w-,;s
- 110 s
-rw_
Conductivity Z Z L_
i t l
z t (unhos/cm)
Temperature (tC)
ZS. z.
.;f.C
.5 yDH tS.U.T
/
100%
. ~
~
I.-
DO (mg/L)
Conductivity
/
(inhbs/cm) 16 Alkalinity (mg CaCO3IL)
( t Hardness i1 (mg CaCOlL) 1 TR chlorine
'4 'O 1 s i1.1
.6S.
Temperature (C)
I D
I pH (S.U.)
zi~o L :4.-5f 14'47-T
\\ _._.,
A
=
100% Intake DO (mg/L)
Conductivity (pmhos/cm)
Alkalinity (mg CaCOs/L)
Hardness (mg CaCOu/L)
IT. S 4-6
/63 1)6 0
'1(d 40.10 ZS. 2-1 Z!. 4)
ZS."3-
- +. I
-C i 111-t I (a I
-1 I
I..4.0. 19 I
'I LIC-3 I
Temperature (C)
I Initial I
Final I
Initial I
Final I
Initial I
Final I
_ ~ ~
n t a
_ F i a_
n t a i a
Environmental Testing Solutions, LLC -
Page 7 of 7 Environmental Testing Solutions, LLC.
Page 7 of 7 Species: Ceriodaphnia dubia Client: Seciuovah Nuclear Plant Date:
oc8 C5X,
-£ Ch.004A-O ae~kZl A EC 1-Day Concen-Parameter 3
-4
-56 tration CONTROL pH (S.U.)
- 7 I.
- q DO (mg/L).016 Conductivity
~
32 Alkalinity I
(mg CaCO3/L)
Hardness
- 4.
(mig CaCO3/L)
Temperature~t Z5b Is%
5Z 2
262.
pH (S.U.)
- 1. i
-(-~ o
-0 27 10.98%
DO m-
.L1 41 I
Conductivity V
4 (prnhos/cmn)
Ztt3Oo
-300 Temperature (0C) ts A
- 1.
zs.!5 25-Z S.
.2.
pH (S.U.)
I. A I
.57
- 1.
I.
1~
22%
DO (mg/L) 0 0
-+.W Conductivity Ozmhos/cm)
.ls~~~~~~~
2.8'4 Temperature (0C)
ZS 5AZ~.
. FZ IS. 7
.. 7.
pH (S.U.)
1.&S4
.CD 9
6-0.)
43.9%
DO (mng1L)
Conductivity 2s (iimloslcm) to Temptrature (0C)
-LIS. t 5
Z.
$.3 ZS5z nH (s.U)
I -a 49?
1 -4.51 1
1 -. 99
.1 43
I-
72%
DO (mg/L)
ConductlvitX (pinhZ.&M)~
I Us~ -+
1~~1~~is.0 I-.
zqee I-+-
i5.e i--
.6
'Z-+
Teinr(erhtUn~k 0Ci'-<
I R
9
- 9.
=
nH (S.U.A o.
I I U-4 I --+.qe I -qqe I---,.------4:
I I
100%
DO (mg/LY.
Conductivity P
V (pmhostcm)
Alkalinity (mg CaC,/L)
Hardness (mg CaCO3/L)
wl.g.
B-0 I
I'll (0
-I 40.
IFL-1
.KS,%
14 1ts I:132 1 14.1 I ef.
I I
Temperature C'C)
- ~ ~
~
I ZZ.4 I
.2 I--Z 1
62 I 2%.-2.
1 1j~ -+.4 801 I-.
k, nH (S.U.)
.9 I
100%
Intake DO (mg/L)
Conductivity (iimhos/cm)
Alkalinity N
(mg CnCO3/L)
Hardness (mg CaCO3/L)
'K.
?S-Z'S.!
Is g I IQ-.e I IPJ Z; 2 EU-I~v I;-
I Temperature (C)
-I--
Initial I
Final I
Initial I
Final I
Initial Il
Environmental Tcsting Solutions, LLC Chronic Whole Eflluent Toxicity Test (EPA-82 l-R-02-013, Method 1000.0)
Species: Pintep halespromelas Daily Chemical Analyses Sequoyalh Nuclear Plant 1W-Treated Client:
Test dhates:
August 05 - 12, 2003 Project number:
791 Reviewedl hy:
(:Conecniration Parameter I)av 0
)I)a I
I)a '2 I)a D3 a 4 I)aI S
I)ay 6 Iitidal I iuaa nftIal ii'nal Initial lInal Initial 1-1nal initial Final.
Initial Initial IFinal piQ (SU) 7.54 7.35 7.50 7.52 7.57 7.56 7.67 7.47 7.56 7.35 7.62 7.X8 7.97 7.85 D)O (mg/l) 7.7 7.7 7.7 7.3 7.7 7.8 8.0 7.5 7.8 7.27 7.6 7.8 7.6 Conductivity (jemhos/cm) 314 26 303 300
... j302 292 311 Contral Alkalinity (mgIl CaCO3)
Hardness (mg/l CaCO)i Temperature (eC) 24.9 25.2 25.6 25.1 25.5 25.2 25.4 25.1 25.4 25.2 25.5 25.0 25.6 25.5 pit (SU) -
7.55 7.30 7.50 7
7.55 7.56 7.68 7.48 7.58 732 7.62 7.84 7.97 7.85 DO (mgWi.)
7.7 7.6 7.9 7.3 7.6 7.7 X.0 7.5 7.8 7.0 7.7 7.5 7.8 7.5 10.98%
Conductivity (junhos/cm) 305 2497 94 2
304 Temperature ('C) 25.1 25.2 25.6 25.1 25.5 25.2 25.4 25.1 25.4 25.2 25.5 25.0 25.6 25.5
- di (SU) 7.54 7.31 7.41) 7.49 7.53 7.56 7.68 7.48 7.57 7.31 7.61 7.82 7.97 7.34 22%
DO (mgI.)
7.7 7.5 8.0 7.3 7.5 7.6 8.0 7.5
- 87.
7.0 7.8 7.5 7.S 7.5 Conductivity (limhos/ein) 292 2X2 2X_2 284 275 Temperature (CC) 25.1 25.2 25.6 25.1 25.5 25.2 25.4 25.1 254 25.2 25.5 250 25.6 25.5 pit (SU) 7.52 7.30 7.46 7.47 7.50 7.56 7.68 747 7.55 7.14 7.60 7.4 7.97' 7.85 43.9%
O)O (mWi.).
7.7 7.5 R.0 13 7.5 7.6 8.0 7.5 i 7.8 6.4 7.8 7.4 7.8 7.5
.c:onductivity (;sliuuoskwo) 264
_l 2558 258 256 258 251 264 Temperjture CC) 25.2 25.2 25.6 25.1 25.5
- 25.
4 25.1i 25.4 25.2 25.5 2
- 25.
6 P11 (SU) 7.51 7.32 7.42 7.46 7.46 7.57 7.65 746 7.52 7.27 7.56 7.73 7.98 7.87 72%
I)O (mtIl,)
7.6 7.6 8.0 80
.4 7.8 78 6.9 7
7.4 7.8 7.6 Conductivity (Lunhosgri) 222 219) 222 218 221_
215 226 Temperature (C) 25.2 252 25 26 25.5 25.2 25.4 25.1 25.4 25.5.0 25.6 25.6 il (SIJ) 7.48 7.29 7.36 7.421 7.57 7.63 7.46,
7.50 730 7.52 7.961 7.85 X)()auglI.)
7.7
- 7.
0 7.2 7.5 7.71
.0
.7...
8...
7.1
.1 7.2 7.8 7.5 Conductivity (pmhosuem)
IN5
_182 IS 133
..jOIR5 IXO
_I7 WaOO%:
Alkalinity (mg/L CaCt0,)
Hardness (mW0. CaCO,)
'iotal Residual Clgiorine (mg/I.)
Temperature'('C) 25.2 25.2 25.6 25.1 25.5 25.2 25.4 25.1 1!
r 25:4
- 25.2 25.5 25.0 25.6 25.6 pI (SU) 7.45 7.29 7.32 7.46 7.38 7.57 762 7.45 i.
.7.46
.7.28
.7.501 7.85 7.95 7.84
)0( (meJI,j 7.71 7.51 7.9 7.1 7.5 7.6
.0
.- 7.5 i
- 0..
8 7.8 MAai47.2 7.7 1 7.4 7.8 7.5 Conductivity(amsho/ena IXU 177
_I3 I xa
.sIXI
-.177
_IR 10IN% Intake Alkalinity (mgIl. CanCO) llardness (mrpi. (:aCO,)
I otal Residual Chlorine (mg'l )_
Temperature (C) 25 2 25.2 25.61 25.1 25.51 25.2 25.4 25.1
' 25.4A
.t
. 25.2 25.5 1 25.01 25.62
I Environmental Testing Solutions, LLC Page 5 of 6 I
[
I L
L vi'.
'S.',k 4
'1'
f Species: Pimephatespromelas Client: TVA / Sequoyah Nuclear Plant UV-Treated Dailv Chemistry:
Date: oe-S-Z-Concentration Parameter a
l 2
CONTROL pH (S.U.)
-4.54 I
S S° l
.5o DO (mglL)
%> 1
?@
-4. q Conductivity l
30 (timhostcm) 3 t-_a
- >8l
'Alkalinity (mg CaCO}IL)
Hardness (mg CaCO/LI)
Temperature(t) C.)
25 A 7.5.
pH(S.U.)L...
q5.
-q-t-S 10.98%
DO (mg/L)
Conductivity (gmhos/cm)
Z91 Temperature (MC) 25 I 5.Z ZS.
.S pH (S.U.)
=1.5
-4
- l S
t9 S9
.3 aS 22%
DO(mgtL)
R.
3 Conductivity
~~~~(ysnhos/cm)
Z9VSZ37.I i(
Temperature (C)
?ZS.l Zs S,
ZS IZ pH (S.U.)
.2 3
- 1 7
Sl 43.9%
DO(mg/L)
Im-0 Conductivity (umhoslcm) 2lSZOE 56 _
Temperature (C)
Z6.L Z.Z 2.1 Z.S ZS.L p_(S.U.)
_.5
).3 Z.
q.YL.
qH' f 4,
- -+.Si+
72%
DOt(m/L) 4.go z
f3 27 _
Conductivity O(mhos/cm)
_z_
ZZ2_
Z N
S. I
't5 ZSrz P
. p(S.U.)
4 SFB s'
-b XeZ ts 100% -' '
_DO__L)'_-I.
XCqnductivity
~~~( pthos/cm) i6 X
Alkalinity (mg CaCO31L)
Hardness (mg CaCO3/L)
TR chlorine Temperature (C) 2S. 7Z Z
Z5,1L lS.
Zx.
t52 pH (S.U.)
-4.3 7-I. Lila
- 1.
-Z 100% Intake DO (ma) 7_.6 Conductivity 111 (Alskahoisn/icty)~ :
83 Hardness (mg CaCO3/L)
\\.\\_
_\\
la Temperature (C)
Z_.2.
ZS-. ZZ Initil1 Finlm~
Initial I
Final I
Initial I
Final
Environmental Testing Solutions, LLC Page 6 of 6 Environmental Testing Solutions, LLC Page 6 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant UV-Treated Date: of s
°93 XkreI~A pJ4 Lo&
0Y%.
Di Concen-Parameter 4 -
56 CONTROL pH (S.U.)
Ste A.~Lo
?
6~
Conductivity Alkalinity (Mg CaCO3/L) a Hardness (mg CaCO3/L)
Teauperature(')
i.A j..
v.S5 2.0 pH (S.U.)
94%
4
- tLZ 71M 10.98%
DO (mg/L) 3 1-.(-
Conductivity 2e04 (iimhos/cm)
Temperatur (*C) 2~...+/-..
z*
s5S 7120 pH (S.U.)
- 9.
H 1~
.D
.2
.22%
DO (mg/L)
.. 5
~
0
.G
~
56 Conductivity A08~
5 Temperature (0c)
.zL 5
IS ZS 43.9%
DO4gL
___1 Conductivtty2' Temperature-M
(')
zsL is~L~
ZSL 1.5.
ZS.O S.2t
(
72%
DO (ma/)
q
+e CondutvV
~TeCe'r2Cire'(*eJ7._._-z s.q
.4 z.L 2Z 100%
-DO (mg/C) 5*
e Conductivity Alkalinity (mg CaCOtL.)
Hardness (mug CaCO-3/L)
Temperature (0c)
Z5 Z.L1 Zs X L-C.7Z Z.5' 7-.0 2S.0.
L5 pH (S.U.)
l.___
100%
DO (mg/1.)*
6 ir
-j'J Conductivityle (pmnhostcm Alkalinity (mg Coco3 L Hardness (mn CaCOA..
~~~TR chlorine (gI.
Temperature
('C)
T&SZ.1
- i.
i..t T, Initial I
Final I
Initial I
Final I
Initial I
Final-I Initial I
Final
36 Environmental Testing Solutions. LLC Page I
of 3 Alkalinity (EPA Method 310.1)
Matrix: Water, MDL - 1.0 mg CaCO3IL Anlalyst Date analyzed o3 l Titrate samples to pH = 4.50 S.U.
2-treantn gnaland multiplier deterination:
l pH of Normality Normality (N) of ES04 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml Na2COJ s 0.05)IE M
(N x 50000)1 100 ml sample
_water reference
_standard
. ml
_n1
.inml 0.251E
-N 5sS00--
> 4.5 SIJ.
number number
._(E)
(acceptable range -0.08 -0.022) 4.6 -b\\5: littlez IU.
._9. _.....6 4_
bt; g Lqhoratqry9cqoi:l standrd:
Reference__ _True value-Sample
.. -- I Alinity(M)l %RSt= MfTVzIOO standard number (TV) volume.
Begin End Total Multiplier (mg C&COAtL) l (acceptable range (mZC&CO31L)
(MD ml ml ml I
I
-90tollo%/0)
\\g~~f 3to ~100 100 l
2 n 225O 9..............:.
-A
,A5
%1 o2.
Duplicate sample Drecision:
,~~
.Sample
'Alkalizity
%/9RPD 3
Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL)
(CS -D) /(S+DY1)I x 100 number (ml) ml ml ml (ecceptabk ange- *10%)
OR-OX64 M S
100 A,
M'5 _6, 9.9 S 59. _ l~
jivli,34ate
+
D 5 Ma~c pJike recovei__
Reference Spike value Sample l Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (Mg CaCO3L)
I (mgCaCOsIL)
(Inl) ml Il ml 1115l36 I1 ltbo (0-Sl~9
- q.
I-l lt
-.Sample alkalinity (B)
Measured spike value (mV)
% R-MV / SVx 100
. j.
(unf.'C&COj/L)
MV A-B I
(acceptable range I F l -
g (mg CaCO AL) g
-?75 to 125%)
Sampr measurements:.
Sample volume Begin End Total Alkalinity Sample number Sample ED (ml) ml ml ml Multiplier (mg Caco/L) 6R0%3 0L I3 tf) I kcn
&6..4 6-6 I
59 SSWVAL O4A zi t,3o3 r b b
1
& ICzA. VZ n&WE bo3 4ri5 lc I
zA 1zOIb I
I I b -I
.cA.0S Zk_3 1.4A 6
A___ l_____t I
l l_
__i_
_1 6
43
- 3.
5
-0 S WLO Ato.
C5 A-Lo \\V 2,6+
60.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Reiewed y: y D-Ivi d:
Date reviewed:
Environmental Testine Solutions. UC Page __Z of 5 Eniomna Tetn ouinLCPg Alkalinity (EPA Method 310.1)
Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst
-,I T Date analyzed V2 -3 1
Titrate samples to pH = 4.50 S.U.
lrant normalitv and pmutiplier determination:
. pHof Delonized water
= 4.5 St.
Titrant.
reference number NormalwL checkl standard number Begin
.l End
-- I -
_ml Normality (A) of HESO
- (C mlNa2CO 3 x.05)/IE (acceptable range - 0.018 -0.022) pH Factor or Multiplier
= (Nx 50000) 100 ml sample x SOO
._0 a, -
- I..
I -
I -
I I
.=
Laboratory control standard: '
-(
Reference True value Sample a
linity MV)
% RS lV I TV x 100 standard number tcr) volume Begin End Total Multiplier (mg CaCOzL)
(acceptable range (tg CiCOA.)
(p ml I mml l
-1_
90 to 110°h) 5
~~~~~100 100 qb~
cq Duplicate sam pi precision:
Sample Alkalinity l
%RPD =
Sample SampleID volume Begin End Total Multiplier (mug CaCO,/L)
((S - D) l/(S+D)2lx 1100 number l) ml ml ml l
__(acceptable range 10%)
bR\\\\63 MX S luo i3.s $31l~~~~~~~~~~~S w
4 3~~~~~
C
.D.p.i.
Matrix spike recove:_
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CSCO 1/L)
(Mg CaCO3L) m(M) ml lml m
/-.4ample alkalinity (B)
Measured spike value (MV)
% R MV /SV s 100 I
.l
,-l (t7g CaOs/L) 7to 125%)
1 -
Qb L
I M A B
( a c t b rae Samek measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ]OD (ml) ml ml ml Multiplier (mug CaCOfl) o636o5~.cte
- 2.
3__
_______16 1
1 3*
0-scb8&S. OS I a2-6A k
0o5.085.6 l
Os 2I I
- 2.
11 cro&O9.O2 5
I_____
\\
R.1S1 3+
=3 3____
\\
l 6.4 -1t#3 16085.b5n a Oi I '2 I 7ti.
\\ I.
1 1 z4
-11zl deoOR &.ol 2..
8 I
S2A t'h 1060969
-Ok 26A 3 6 t~~~~-
- (o4.g foo Reviewed by: I rI kI Ii I
O Z
Date seviewed: l 5-%~3
Environmental Testing Solutions, LLC Page 53 of 3 It I
[.
Alkalinity (EPA Method 310.1)
Matrix: Water, DL= 1.0 mg CaCO3I Analyst Date analyzed [i Titrate samples to pH = 4.50 S.U.
Tifrant and pH of Normality Normality (A) of H3SS0 4 pH Factor or Multiplier Delonized Titrant check Begin End Total
= (
3 s O.OS)/E
= (N x 50000) 100 ml sample water reference standard ml ml ml
= 0.2 x__
N 4.5 S.U.
number number (E)
(acceptabl rag - 0OIB -0.02
-Laoratorycontrolstandard:
Reference True value Sample Alkalinity MV)
RS MV TV x 100 standard number (TV) volume Begin End Total Multiplier (me CaCoiIL)
(acceptable range (me Ci CO/L)
(ml) ml ml ml 90to110%)
\\i~~s t3 lo 100 100 56 5 4tr>-
q 8 Ir -
9!t l!o)
Duplicate sapeprecision:
I Sample Alkalinity 6RPD =
Sample Sample ID volume Begin End Total Multiplier (mig C&COi/L) {CS - D) Il(S+DY21) 100 number (Il) ml ml ml (acceptable range -
10%)
a3 otes
@s ^t iE \\ to n S p 6.6 9. 4 S b4 5 -m owz 014,pte.
1 '.a lb-b Lb.
- . 9 s
t 3
,_5= B (5
2.
9 9up1c-t D
Matrix spike recoerv:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume lBegin lEnd lTotal lMultiplier (m
ttCICoJL)
I (maCaCOI/L) m()
1 l 1 ml ml I
l f.4atnple alkalinity (B)
Measured spike value (MV)
%R=MV/SVx100 l1:
(ni(CaCO iL)
- AVA-B (acceptable range l
l (me Caco /L) l
" 75 to 125ff.)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ED (mlD ml ml ml Multiplier (me C*CO/L)
I5600 I-X /1 10 10 91 1
11 I5 ea 1L 4o 184 I
1 1
1-_
+/--
- _1---_1_1_1-_11 Reniewed by: i %
W I
Datereviewed: l9
Environmental Testing Solutions, LLC ftge of 2.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [
Z I
Date analyzed VI 6 J
Tira norma and multi lier determination:
Titrant Normality checkl Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml
- 0.21E (N s 50000Y 50 ml sample number number (E)
(acceptabke rnge - 0.018 -O.OZ2)
CNs NOO0 II UJLD4Q I I MD'O1 C 1~I04 I Vrb I 't-I O0l.
-0(o I
7tQ-P I
Laboratory control standand --
Reference
- -~True vlue -Sampl V
% RS-uMY JTY s 16 standard number (TV) volume Begin End Total Multiplier (meg C&COtL)
(acceptable rane
.....- ___. -gMCCaCOV-L)
)
-ml
-m
-o---
-I I9OtolO%0)
- 40.
50 7
T-Duplicate sample precision:
Sample
.Hardness HsURD Sample Sample ]D volume Begin End Total Multiplier (mg CaCO/L)
((S -D) /l(S+Dy211 : 100.
_umber (mI) ml ml ml (acceptable range 10%) I DC'B-M-3
,4s (C
A l
lD
(* V 2.06 s_
Duplicase
- 4.
D Matrix spike recoverV.
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (lmg CaCO3/L) l (mreacCo/Lj) (ml) ml ml ml Sample hardness (B)
Measured spike value (MR
= MV/ SV x 100
.S~ 4 Ic, <^, (mg CICOA/L)
MVL-A-B (acceptable range Sampl Imeasurements. -
Sample volume Begin End Total-Hardness Sample number Sample ED (ml) nml ml ml Multiplir (me CaCO3/L)
(should be-0maCaCOA/L) l Q
5L l
b ThA0 ID 1Obow lff45 L10 A i
2.t131v1 U-AI R--
IDES--O'sO I SSXO 1AI 126 J,
-MI Z3 l-\\
l A
r5 c 0D 1K5 I
I-
&9 13 1
l3 IO0OR O3 146SzO 1
1 1qLA H5612Z I I
I ;
I7-A CA 1O.S e,-SAb AOI I
7O4.0A-l 8
c028n7.
nds I
A7 l
-1 OW6pi. O(O 2..
7, 3A,.
Note: If >I5ml of titrant is used. sample must be diluted.
RevieWed by: [l Z
D e r Date revie-kd
q3 l
Environmental Testing Solutions, LLC Page
- 2.
of t I
I I
I.
4j.
Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L I
Date analyzed O3 TlfgUzdpormalitj and multiplikr determination:
- I I
S I
Titrant reference number Noiiii Begin End
-standard
_ n br number
- 1 I
I a
.i
/
Laboratery control standard.
f:
Reference True value Sample Hardness MV)
-% RS = MV I TV x 100 standard number (Tc) volume Begin End Total Multiplier (mg CaCO3IL)
- (acceptable range (Mg CAC O3L).
(MI ml ml ml 4 110%)
I\\5S -c5 40 50 C9
%41
.o Duplicte s precision:
Sample
.Hardness
-i.RPD -
Sample Sample ID volume Begin End Total Multiplier (mg CaCO.4L)
((S -D)II(S+Dy2) )
100 number (mla ml ml ml (acceptable range - d IOY)
I
'S~~~~~~
OwtA o1 fiQa _ 3 5b Q. Q mto &3.
to s'
2 Duplicate a t__3_
D Z6 2J b\\
Matrix spike recover Reference Splie value Sample I
Spike hardness (A) standard number (V
volume Begin End Total Multiplier
(-g CaCOAlL)
(ml CaCO,/L)
(ml) ml ml nml
-^
l Sample hardness (B)
Measured spike value (MV)
%R -
V /SVx 100
-o \\
.1..-
l_
(mg CaCOu/L) l MV=A-B l
(acceptable range I _-
I
- >=.
(mg CaCOV/L)
-75 to 125%)
Sa~ple measurements:
Sample volume Begin End Totall Hardness Sample number Sample ED ml mnl ml Multiplier (mC CaCO,/L)
Blank (should be -
mg CaCOs/L)
I___I_____
lOboes.6 19A 1-
\\L I s I
1.l8:4l 2/10.
1 b.L - Wi ns20ta
- 07.
A L
l I.6 1
1 3?
l_
l_I 5E.
- l.
l I__
I lX 16
.4 o ~
\\1L
-0508M.6S 1 1
3 1
l 1
k-6 1
?-
-1 6 oR 'so5.
67 5
-tCs 5.q
- 10 1^t~~~~~~~~~~ot~~51 1
1 I.
I' 1
Notc If >15ml oftitrant is usd sample must be diluted.
Reviewed by: [I A
I Date reviewed ji-Ozc3i
Environmental Testing Solutions, LLC
&f71%
ZD-5-
44 Page Page.
of Total Residual Chlorine (EPA Method 330.5)
Matrix: Water,-MDL = 0.10 mg/L Meter: Accumet Model AR25 pHI~on Meter Analyst l AA
]
Iodide reagent:
I f o8 I
Date analyzed D
Acid reagent:
AS 1Ijf j8
.- - --Calbration: - --.-
[.
.~
m 0.10 ng/L
-- lOani/L-m lReference standard umuber l -
51;I q I
I Note: For samples with residual chlorine or > 1.0 mgL. the calibration range must be adjusted to et the chorne levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS=MVITVx 100 number mgfL)
(mIgL)
(acceptabIl range -90 to 110%)
1 55t3 AC.
0.50 O.
Z1:)-tq Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD ={(S - D) /[(S+D)/211 x 100 number (mglL)
(acceptable range -
- 10%)
o05e~s.ot Cerr ' A.e-, Lb
\\
~
O.bs~
t=0EMUR
-Duplicateep D
-. 5 Sample measurements:
Sample Sample ED Sample characteristics Residual chlorine number (mg/L)
.Blank (should be = < 0. 10 mg/L) 4 6 06 3 CZ Ot~o5.C A2..
1J~l'(~Xd Crp f"'
'l (c.ci~b 3o4 0342z)S.J<
0 -lot _g>t, MwP&A.44, kakii-e fse4; l¢t~vtx 00l30 03 0o os.os 5Q l n1o-Tv p I cL
.~IL.
~tO.
Note: Allsamples were analyzed in excess of EPA recommended holding time (l5 minutes) unless otherwise noted.
Laboratory control standard:,
Reference standard True value (TV)
Measured value (MV)
% RSMV/TYs100 number (mg-L)
(mg1L)
(acceptable rangee -90 to 110%)
15S 134 o0.50 O.5s Oo 1.
Reviewed by Il-A
\\
zII Date reviewed l 6CCt0Ld
-5 1.3 'l.
Environmental Testing Solutions, LLC.
Page IZ Page I
of l
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL - 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst li Z.]lIodide reagent:
l L18iI]
Date analyzed 7 Acid reagent: [JjS~ jjj3
- .. Calibration:
.=
=1 O.O1ng/L I- - 1.o0mgL --.-
Reference standard number l
fjSS I b I
p$55131I
. -t.. - -
._Note: for sa les with a residual chlorine of > 1.0 mgftL the calibration range must be idjusted to bratkit the chlorine leve-os the sa-plcs Laboraory control standard:
Reference standard True value (TV)
Measured value (V)
% RS - MV I TV x 100 I number L
(mgfL)
I (mgL)
(acceptable range - 90 to 110%)
lgt;34 0.50 in os 4o I Ot. (4%
S7 Sample measurements:
Sample Sample 11 Sample characteristics Residual chlorine number (mg/L) o '
-Bl Ia n (shuld be =
0I0 mg/LL 1
tBORO-7.05 sinin 100 no "U&Z' l~aots 05071.C a
n-l potlv UA2 O3%$31. 01 iXA i -
(rSQ rc*.
lS exg,,
CII>Z
~
o-oooe o--Aaal ab Nk ;nC-Q~kA CP._____
_ o-crZ--
- Ai C
Aut
,2nL c
O'OO ZZq OrBCAO-7. a 4
PP Ad 05CI&61. 0
-r4 J
'0&
I ltJ Pik tta c
<oct, Note: All samples were analyzed in excess or EPA recommended holding time (3 5 minutes) unless otherwise noted.
Laborator control standard:
Reference standard True value (TV)
Measured value (MV)
%RS MV I TV x 100 nurnber (mtgL)
(mgfL)
(accetable range - 90 to 110%)
1W65 IEU R0.50 I0z a 7OZ-%70 Reviewed by I I l
Date reviewed I _
- 2
-b5).6 *I Environmental Testing Solutions, LLC Page 1i Page I of i
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L
- Meter: Accumet Model AR2S pH/Ion Meter Analyst [
I I]
Date analyzed t) e 09j-j0 l
Iodide reagent: [Ii ROB Acid reagent: LIlaIII Calibration:-
Q10 mg/L 1.00 mCIL I
Reference standard number
-Nowa For sanples with a residual chlorine of > 1.0 mgtL, the calibration ange must be ad4usted to bracket the chlorine levels of tie sampleL Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
%RS-MVITVx 100 number (mg/L)
(mewL)
(acceptable range - 90 to 110%)
0.50 0.5)O iOoI.
Duplicate sample precision:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = ((S - D) Il(S+D)12]) x 100 number
__(metL)
(acceptable range - + 10%)
.0 c9i
_ _ _ _ __I_-_ _ _
D i u
_ _ _ __4_
Duplicate ;
D 40.0141 Sample measurements:
Sample Sample ED Sample characteristics Residual chlorine number (mgL) l.'Blank (shouldbe=<0.lOmg/L) 3
.o-oC)5 0608010 GG0-4G I
TF-e S-s 1
fVc d $ O '.c 3 :
Ae.J CL). I d O rl.
7 Note. All sampleswere analyzed in ecess ofEPA recommended holding time (IS inutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV)
Measured value (NMV)
% RS MVITV x 100 number I(Mg/L)
(me/L)
(acceptable range 90 to110%)
- 4.
Revierd by l Z
h Date reviewed E l
Sequoyah Nuclear Plant Biomonitoring August 5-12, 2003 Appendix D Reference Toxicant Test and Control Chart i,
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.0 0.9
-.-. -. O._.9 0.8 0.7 0.6 I ~
I I
l l
l l
l l
l l
l l
l l
l nI E
State and USEPA Mandated Control Limits Limits Set'Accdrding t'o f-2 StdiDeiations '--
j
~4 N
0.5 0.4 1.0 0.9
- 1 I
II I
I I I
I I
I i
I I
I I
I i
I
.I USEPA Warning and Control Limits Limits Set According to Iot and 25'h Percentile CVs
.,I I
I I
0.8 0.7 0.6 0.5 0.4 I
I I
I I
I I
I I
I I
I I
I I
I I
I T
% ;~;p~;9 est OV 0?
dt Test date 7-day IC2s =25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC2 )
Warning Limits (mean IC25 S
. Control Limits (mean IC25 i S.
or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Tos for PXmephales promelai
\\'using Moderately Hard Syntheti State and USkPA Laboratory S
Controla mitt
'S^§*
.7et number Test date 7-day IC" CT (WL Ka)
(gL Ka) 2 3
4 5
6 7
3 9
10 1I 12 13 14 is 16 17 is 19 20 10-01-02 10-0102 10-03-02 10-15-02 10-22-02 11-05402 12-03-02 12-03-02 01-07-03 01-14-03 02-04-03 03-1t-03 03-1 -03 04-02-03 04-1543 04-29-03 05-29-03 06-17403 07-1543 0
5-043 0.60 0.47 0.53 0.66 0.64 0.59 0.61 0.52 0.64 0.61 0.64 0.65 0.64 0.50 0.56 0.59 0.64 0.60 0.65 0.53 0.54 0.53 0.56 0.51 0.53 0.53 0.53 0.53 0.59 0.59 0.60 0.60 0.59 0.59 0.59 0.59 0.59 0.60 0.59 0.09
- 0.35 0.73
.0.06 0.47 0.07 0.40 0L67 0.06 0.47 0.03 0.40 0.73 -
0.07 0.50 0.03 0.42 0.74 0.07 0.51 0.07 0.44 0.72 0.07 0.51 0.07 0.45 0.71 0.07 0.51 0.06 0.45 0.71 0.07 0.51 0.06 0.45 OJI 0.07 0.51 0.06 0.46 0.71 0.07 0.52 0.06 0.47 0.71 0.07 052 0.06 0.41 0.72 0.07 0.52 0.06 0.43 0.72 0.07 0.53.
0.06 0.47 0.72 0.07 0.52 0.06 0.47 0.71 0.07 0.52 0.06 0.47 0.71 0.07 0.52 0.06 0.43 0.71 0.07 0.52 0.06 0.43 0.71 0.07 0.52 0.06 0.43 0.71 0.07 0.52 0.06 0.43 0.71 0.07 052 0.60 0.60 0.63 0.65 0.65 0.65 0.65 0.65 0.66 0.66 0.67 0.67 0.66 0.66 0.66 0.66 0.66 0.67 0.66 0.11 0.11 0.32 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.13 0.13 0.12 0.12 0.12 0.12 0.12 0.13 0.12 icant Control Chart C Waitter Laboratonr i
USEPA Control Umltm Si a rndin LUmits S.,
CT-SAA C`T+SAM CT-SA.M
'Cr+SSAM 042 0.65 0.20 0.33 f 0.74 0.24 0.42 0.65 0.20 033 0.74 0.24 0.45 0.63 0.21 0.35 0.73 0.25 0.46 0.70 0.22 0.36 0.30 0.26 0.46 0.70 0.22 036 0.30 0.26 0.46 0.71 0.22 0.36 0.J1 0.26 0.46 0.70 0.22
.36 0.30 0.26 0.46 0.71 0.22 0.36
,' 0.31 0.26 0.46 0.71 0.22 036 0.31 0.26 0.47 0.71 0.22 0.37 0.32 0.27 0.47 0.72 0.23 0.37 0.32 0.27 0.47 0.72 0.23 037 0.33 0.27 0.47 0.72 0.22 0.37 0.32 0.27 0.47 0.71 0.22 037 0.33 0.27 0.47 0.71 0.22 037 0.1 0.27 0.47 0.72 0.23 Q37 0.32 0.27 0.47 0.72 0.23 0.37 0.82 0.27 0.47 0.72 0.23 0.37 ?
02 0.27 0.47
- o.
0.23 0.37 0.32 0.27 USEPA Control Limits CT -SA b CT + SA.
CV.
0.30 0.29 0.31 0.32 032 0.32 0.32 0.32 032 0.32 0.33 0.33 0.33 0.32 0.32 0.33 0.33 0.33 0.33 0.73 0.13 0.77 0,13 0.82 0.15 0.34 0.14 0.34
.0.12 0.QS 0.11 0.34 0.13 0.35 0.11 035 0.10 0.36 0.10 0.36 0.10 0.37 0.10 0.36 0.11 0.36
.10 0.36 0.10 0.36 0.10 0.36 0.09 0.16 0.09 0.36 0.10 Atr:
7.d IC, 7-day 25% inhibition concentraion. AA estimation of the concentration ofpotassium chloride that would cause a 25% reduction in Pirnephal growth for the test popultion.
CT Central tendency (mean IC2)J' S - Standard devation of the lCu values.
Laboratoy Control and Warning Limits Laboratory control and warning lints were established using the standard deviation oa the 1Ct values corresponding to the 10th and 25th percetdile CVs. Tie ranees a or e stringent than the ontrol and waning Eit reconunended by USEPA for the test tethod and endpoint.
S,,- Standard devitdon corresponding to the Ida percentile CV. (Sa -0.12)
Sn-Ntanda ndeviation CorrespOndull to tle 2-percentileo Cv. (tx't, - U I USEPA Control nid Wamnhng Limits SA,, - Standard deviation corrnpondng to the 75" percentlle CV. ( S
-0.33)
S," -SStandard deviation corresponding to the 90* percentile CV. (S
-0.45)
CV I Coefficient of variation of the IC" values.
I I
iII III USEPA. 200. L mUndfsat KAn ACeaWung for Method Variability in Whoet Effloem Tiexicy Applications tUrAr the Hational Pollutant Dtachaq Elimineinati Pfolum. EPA4533-R-OO00-M.
US £nviraunenals Protection Asency. Cnlmad. OH.
00it"Au akrobsedirsaAquatde 8ioSyuteu. Inc.
.0-ld I
i I1 Oft-O")AI
Environmental Testing Solutions, Inc.
Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas uiing Moderately Hard Synthetic Water 1.0 0.9 0.8 U
N4 0.7 0.6 0.5 I I I
I I
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I I~~~~..
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
0.4 0.3 0.2 4
ys Iw
'O V
° Test date A
V V
11
!%
"ej j
21;t
7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC2)
..- Laboratory Warning Limits (mean IC S
s Laboratory Control Limits (mean IC25 + SA.25' SA.25= 0.21)
JUSEPA Warning Limits (mean IC2,
- SA7 S, S.,
= 0.38)
USEPA Control Limits (mean IC2, + S,.,
SL90 0,45)
.~~~~~~~~2
_.0 A9
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Test number - Test date.- Conta
._Control Mcan_ -
C-Survival Growth
=
CT -
MSD
--PMSD CT - -
(%)
for Control Growth (mng/arve)
(m g/larve)
CY.)
for Control GrowthCV (*)
(C/)
for PMSD (%)
I 10-01-02
_97.5_-- 0.750 2
3 4
S 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 10-01-02 100 10-08-02 97.5 10-15-02 100 10-22-02 100 11-05-02 100 12-03-02 100 12-03-02 100 01-07-03 100 01-14 100 02-04-03 97.5 03-18-03 100 03-18-03 100 04-08-03 100 04-15-03 100 04-29-03 97.5 05-29-03 100 06-17-03
^
97.5 08-05-03 97.5' 0.975 0.929 1.037 0.822 0.874 0.852 0.668 0.886 0.677 0.933 0.838 0.803 1.083 0.892 1.021 1.005 0.888 0.910
,,1.,992 0.862 0.885 0.923 0.902 0.898 0.891 0.863 0.866 0.847 0.855 0.853 0.849 0.866 0.868 0.877 0.885 0.885 0.886 0.897
--18.4 12.7 8.0 16.9 10.6 2.8 9.1 10.4 4.1 3.0 14.1 8.0 21.3 6.1 17.0 6.7 7.9 4.2 6.7 6.5 15.5 13.0 14.0 13.3 11.6 11.2 11.1 10.3 9.6 10.0 9.8 10.7 10.4 10.8 10.6 10.4 10.1 9.9 9.7 0.19 0.13 0.18 0.23 0.13 0.12 0.12 0.15 0.14 0.07 0.15 0.15 0.21 0.09 0.17 0.18 0.11 0.18 0.12 0.13 25.4 12.8 19.5 21.9 15.6 13.8 13.7 22.4 15.7 11.0 16.5 18.5 26.5 8.0 18.7 17.5 11.1 20.7 13.7 11.8 19.1 19.2 19.9 19.0 18.2 17.5 18.1 17.9 17.2 17'1 17.2 17.9 17.2 17.3 17.4 17.0 17.2 17.0 16.7 Not/e.
CV - Coeffigient of variation for control growth.
On average, the CV for control growth is 9.7% in Environmental Testing Solutions, Inc. Pimephales chronic toxicity tests.
Lower CV bound determined by USEPA (10' percentile) - 3.5S/.
Upper CV bound determined by USEPA (90"' percentile) - 20%
MSD - Minimum Significant Difference PNISD -Percent Minimum Significant Difference PMSD is a measure of test precision. The PhlSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole etfluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions. lnc. chronic toxicity tests when a toxicant reduces Pimephales growth by 16.7% from the control.
Lower PMSD bound determined by USEPA (10* percentile) - 9.4%.
Upper PMSD bound determined by USEPA (90* percentile) - 35%.
CT - Central'Tendancy (mean Control Growth, CV, or PMSD)
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
Orgaai~irs obrained from Aquatzic BiaSyttemir. Inc.
O-S0.l 0a-05-3.xl
Environmental Testing Solutions, Inc.
Pimephalespromelas Control Growth and Coefficient of Variation in Potassium Chloride Chronic Reference Toxicant Tests S
=
C~
W
.C r L.o 1.50
. 1.25 1.00
- 0.75 0.50 I
I I
I -
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............ ;W- { -...................................................................
Minimum Acucptance Criteia (0.25 mg per surviving larvae)
I,
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I 0.25 C
0
- O*-
1.
L.0 a-I0 4
QU 40 30 20 10 0
1I~~~~~l~~'1 O O
C O
O~~~
0 4C Test date Control Growth or Coefficient of Variation (CV)
Central Tendency (mean Control Growth or CV)
Control Limits (mean Control Growth or CV *2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant ControlChart for Pimep hales promelas
- using Moderately Hard Synthetic Water 40 -
30 420 -
10 i
.~'9p" 0
,I, l
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'4o t~/
9j 9
4 O20 2
0t 4
° 2
0t 0t°° t,° 58
~~~~~~~~~Trctdte i~
I
+-
PMSD = percent minimum significant difference. PMSD is the minimum significnnt difference.
between the control and treatment that can be declared statistically significant.
- Central Tendency (mean PMSD)
Lower and Upper PMSD Bounds L.ower PMSD Bound (10' percentile).= 9.4%, Upper PMSD Bound (90&'percentile) 35%/6 (Lower and upper PMSD bounds were determined by US1EPA for the method and endpoinL)
Environmental Testing Solutions, Inc.
Chronic Wholc Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control VcriflcatiqnAof Data Entry, Calculations, and Statistical Analysesi I
...., )5
. I
>33~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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I rJ1 I
II Test number.
PpKCICR 6 53 Test dates:
August S.12,2003 lRmlWed bry I~~~~~~~~~~~~~~~~~~~~~~~
\\:
/1I ce"aslroflls (UV.L 1kaptie lhixid mu brb-r 1
Fiust sambr.
P dgb F
FBst taw
- Larasa Laras. wd1gb 1(mg Watbi allat salblr Meass r
Mlval tea1 w
lde~k t
o.f3s.
rued s fr Yca) km.
kv.
(ml wA.
of kivae (mg)
Warnlaeso.
0')
Ieard.%)
A 10 10 J.. 119 25.700 10.5tl 2.0532 Ccntrcl n
20 30 14.393 26.880 1 L"?
1.1987 c
1o 9
15.129 25.730 10601 1.0601 6
p D
o0 10 144.724 25.230 10.506 1.0506 R
0o 20 14.681 25.250 10.569 I.0569 300 F
20 20 14.600 23.110 3.S50 0.3510 300 100.0__________2 9.,
G 20 20 15.205 25.390 20.335 2.0335
- 36.
.14 lit 0
20 125.156 25.250 10.094 1.0094 I
o0 20 15.253 24.750 9.497 0.9497 436
.5~
~~
20 20 14.939 2S.090 20.202 2.0202 06052 443.
45e8 i:100.0 9
2eoso~tos~ssz 0.9542 4.4 12.6 K
20 20 14.692 23.770 9.073 0.90~783 L
20 o
I_
1 4.72 24.210 9.492 0.9492 M
20 a___
14.754 21.4SO 6.726 0.6726 600 N
10 7
14.795 21.920 7.125 0.7125 366 6e762 14.1 35.1 o
10 7
14.933 21.040 6.052 0.6052 20 to
_o 14.367 23.290
.421 0.,423
- Q l0 4
1 24.333 17.920 3.032 0.3032 lR 10 6
14.632 17.680 2.993 0.2993 S
10 5
15.102 13.500 3.398 0.3393 T
10 3
15.054 17.370 2.316 0.2316 t
U 20 2
15.096 16.370 1.274 0.1274 9o0 V
to 0
0.000 0o000 0.000 0.0000 7S 06369 16A.4 96.6 2 0 I
15.037 25.290 0.203 0.0203 X
20 I
0 0.000 0.000 0.000 0.0000 Donnell$ MSD value:
PMSD:
6.1283 11.3 htSD) =
mlirmaSigniflcant Difference PMSD-Percent "fimnnsmnSignificant Dilferenc I.
I..,
I i
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I PMSDia
- a ureoftes prcaiso.
Te rMSD isthe nininrmpercnt di enmbetwn dw eanteoladreat tht ean be dedlaed statisticaly signilicant in a whole efluem toxicity tes. On swea, a sipificust difference octcas lbr EAviomental Testing Solutions, Inc.
dunct ieily teswhten ticwredce Pipales owth by 167%fir theonriol tdetenned i
t tgh e ieoxlant testingt Lower PSD bound determnined by USEPA(lOth percentile) - 9.4%
I Uper PMSD bound dtemned byt USEPA (Oh percenile)
S
- 35.
I I
Mm lower and upper bounds were calculated by the LtEPA urins 205 taW cqducted firam 19 laborstrice tar Pimephbles powth in chronic reference toxicait tamla j
WIP.
200. Uthdaning n Acmting ar Method Vanability in Whole fmucn Toxicity Aplicions U the National Pollutant Diachge 0ininticn Pron EPA4
.-R.00a3. U8 E m4ironn tl Protection Apency.Cincinnati.1 0,.
Orgtrnisms obf ajied from Aquatic IioS'ystems. Iunc.
30.0.
I... I I
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OJ 05 03.xls
ironmental Testing.Solutions, Inc.
Page I of 5 roninental Testing.Solutions, Inc.
Page lofS Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number:
'3 Dilution preparation infcrmation:
Comments:
l KCl CHM number:
C(A 661 Stock preparation:
0 I Dissolve 0g CC in IL Daonezed watera_
Dilutionprep (mglL) 300 450 600 750
- 900 Stock volume (mL) 6 9
12 15 is Diluent volume (mL) 994 991 93S 935
- 982 Total volume (mL) 1000 1000 1000 1000 1000 Test organism information:
Test information:
Organism age:
ZS Tr Z.S oiqjg OLb Randomizing template:
R )OPL&
Date and times organisms O
C 11M lM V550 Incubator number:
were born between:
Is__3 Organism source:
A gm B d-eo-ab Artemia lot number:
6la!
Tmansfer bowl information:
pH Temperature =
°C Total drying time:
19lk~S 1.tO l.4.cG DatelTimein:
at-4M ISMO Average transfer volume:-
i O t
Date I Time out: at t3 e M
- Oven tem perature:-
Lt 1C Dailyfeeding and renewal information:
Day Date Morning Afternoon Test Initiation, Control water Analyst I k feeding feeding renewal, or batch used A )k.--.
f__f___A time time termination time tt1; 0o 4
iso (t-ole 2
1 1
tZOC
'_________z b
A-t-
3 t
2
__a c&Clt O
~
-Ce 3
j L!
3 at S oss-Ots0 l200~c
,zz-t. -
Ok 6
O&.lO-O3 6f'S lItsS(
Oto¢-W d
7 ____it-a Ot_
tS' tz 0 Ci I Control information:
I Acceptance criteria I Summary of test endooints:
%Mortality:
.2^S Z;
s 20Yo
_7-day LCso 1l7 l 2-Average weight per initial larvae:
- t. 09 l'l NOEC Average eweight per survivin larvae:
I.IZ3 2 0.25 rnglarvae LOEC 4SC ChV 3I d
ICS TF52.
I's
vironmental Testing Solutions, Inc.
Page 2 of 5 PpKCICR Test Number: 15 Survival and Growth Data Day CONTROL 3nKC C
I-45mKCI/L
...-____ A
-.B
-- C D
E F. --
G l-H l-I--J- -K---l -L O0
/6 //0 0/0 l /0/
'l6
/
to 0/0 /o
/0/
/0
/c0//
/'
. 2 b
_C
_10 Icy lb4 IO)
/(-
to-,
IC) to 10 1
o-i it_ 10
'l 1
1 /0 Jo
/0
/0 /0 /0 4
4________O
/0 XO q() 1 0
) 10 I0
/(
/0
/0
/0 l0 1
0 40 1(O-)P O
{
i to 1(
7
/0
/0) 1 I
iO.
/0 1(-1
()
'0
/0 1
(0 A -Pan weight (mg)
B-Pan+Larvaeweight(mg) 0 p1 C-Larvae weight ngd )
A
\\ -
-A-B 4'~~~~~~~"!
VI O lb
%b I PUb Weight per Ltinibro S\\8 f~0t50a; 0d o6 tarvae (mg)
Co~L C I InitiaJ number ofdrvde
- O' O'
O O
Average weight per initill numberotlarvae(mg)
Calculations and data reviewed: 4.
ivironmental Testing Solutions, Inc.
Page 3 of 5 i,
PpKCICR Test Number: 13
-- / _
".i
%D.
Weight per hital number of a
larvae(mg)
- e.
a I
- Ca IInitial number of larvae.,
Average weight per Isttal number of larvae (mg)
Calculations and data reviewed:
e
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fisb CrGwth and Surytral Text.7 Day Sutr al Sut Dew V
30 Test ID:
PPKaCIR SAMple D:
IEF-RtTioant End Date V12103 Lab ID:
ETSEm.Teotting Soldu Sample TYPC M-Patassn clbtlo" sais Duet PmwcLt CHRONIC.(PA4210-R24l)
Tat Species "PPimephasl prtmuets Comments:i Coec-ingL I
2 3
4 DCArw 1.ec 1.000o Q1900 L.0000 300 1.0000 1.0000 L.OWC IM0 450 M0 ewc 0
1.00wc Ic00 600 0.300 0.7000 0.7000 1.0000 750 0.4000 0.60 o0500 0.3000 900 0.2000 0.0000 0.1000 0.0000 Traadovui: Arcola Square Reet Rank I-Tapled Numiber Total Cone-safl.
&tea "osu Man 11a Miax CV%
4 a
CrtalRasp N~umber DC entm 150 I eoce 13713 1.260 1.4A20 5J942 4
300
.. 100 1.0256 1L4120 1.4120 1.4120 1900 4
20.0
.10.00 450 1.0000 1.02.6 1.4120 I.4t20 IA12o 0.000 4
20.00 10.00 MO 01000 01205 1.1254 01912 1.4120 17102 4
12.50,
0.00 50 f-- 45o
--.f4615
--- 0.734
-.- QS796 _
0J61
.- 17.42._.4._
tam.0 10.00 90O
.70 0.0769 Q w m7 Q.1sS 046 3.294 4
J0.QQ 10.00 I
-40 a
40 0 '
40
£ 40 22 40C 37
.40 Aux~lw7 Tests Stastic Crftical Skew Kurt sw$o.Wi&, Teat katcsnae ac disi>botion (
.01) o0s764925 0.14 0.19S152 2M3533 ca*it ofwuriance annot be condmed NyaottiesIsTe 1t(i-tall.0S)
N4OEC LOEC ChV TU Sicerrs M y
Rank Test 600 7J0 670.1203932 Treiaieus es D.Catrol iaglatunt LlkallbtoPrbit Pauameter Value St 95% Flduci Urults Control Cwq Critica P-Value Mu Sigma Iker Cope 33.09419594 20326WIl6 9.110223195 17=71L6869
- 0.025 1.991210 7114724922 0.57 2.355052417 1076369714 6
Intecept
-32316147
=2151626 -3.794718
-20.1744446 TSCRL 0.003763746 0.00923663 4.00934005 0.026367541 1.0 Point habits wfl.
95%
frducia Umdts 0.9 ECOI 2.674 475.7622334 390.4425536 530.66537 ECOs 3.55 5363341931 462.5037421 3.4314204 0.8 ECto 3.718 S71.165463 0S.7319113 614.2371026 7
ECIS 31964 96.8913365 53610039-636.3514.59 EC2O 4.353 617.70071t 562205614 65S3S377 0.1 EC25 4.326 636.1241709 5s1.2249964 671.606509
[s EC40 4.747 6S5.02157SS 644.10SS214 73.39897 ECJQ 5.000 716.22ss676 793290179 751.201692 0.4 ge S.253 74s359835 7132543012 7165IN013 8.3 EC75
. 5674 W6.4233161 767.7689619 U39 J0443 0.2 ECSO 442 130.47ss721711.4176906 197.1702224 ECIS E l 359.413363 32.2913745 939.0962466
.11 Ecso 6.*2 397I21IOL.812.361633979
.9...09169 0.o EC95 9.645 9l6.4651 764 887.62S2ss 10b7.933721 1
10 100 t0oo 100oo ECss
- 7.326.11123A01 976.6395442 1237.679427 DosemL CeseRespouse Plut 0.7 0.41
.13 0.21 0.1 Oq~anints obtaimwd fim Aquatic Biogystemx In 1.53 d OS40543 xl6
Environmental Testing Solutions, Inc.
Statistical Analyses Larval Fish Growth and Survival Tess.7 MY Growth Swi Date~ VY103 Tat Mt rpICICR SaZMple lD.
ZEF-RfTedcani Ld Date:
3112i3 LAb ID:
- MEAV.LTvsting stolns IazPle Tyc KCLfthaduiwucblotide Sole* Oac:
hatoostL CHRIONIC.(EPA4214402.013)
Tast Spac~ss Pl'4i"ebales promclas Conunens:
Cowc.VgL 2
2 5
4 D.Controt 1.0581I 2.1987 1.061 1.0506 200 1.0569 0.110 2.0183 1.009 450 0.1497 LOIN0 0.1071 0.949 AM0 0.616 0.7125 0.6052 0."42 150 0.3032 0.299 0.M"9 02316 Om0 0.1214 0.0000 0.0203 0.A0M Ttaussons: Ultvansnrcutd CHL 10111 Men e
I; n
PAMQ Wa Max CV%
D.C4aL IC919 1.0000 10919 1.0506 I.197 6M 4
300
- 040 0.9012
. 0.9640
.. 0.e510 1 es69 9.24 4
- 4a0 09542 0es739 0.9542 007s 1.0101 4.414
- 4
- 600 0.7CS2 0.646 0102 0.6052 0.123 14.092 4
0.2936
--0.689.-o0.2936..- 0.2316 -.0.339 _z
.13
_ 4 900 0.0369 0.0338 0.0369 0.0000 e.1m4 165392 4
I-Taied l.Isealc 1Stat Crtcsl IASD Mean
- K-Menu 2.0929 1.0000 m3.
2290 0.121u3 e94 0.9012 245 2.290 0.123 0.9542 739 Also 2.290 0.1213 0.7002 1.644 0.2936 0SIM 0.0369 0.0338 AuSIl3 Teu SdstIk Critical Skew Kut
.Sliapiro.Wk Ts uini nornal distrizitdo C(p o 0.01) 0.976972163 0.44 0.11246245 403144914 BardtsTat stindlcaten ualvuiancest Qs0.5) 193307364 11344I9201
=lypasisTort(
_C TU MS Du &ISD MSB MSE F.Preb W
DuOne sTet 300 450 367.4234614 0L2n274997 0.117411394
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Peint mRWL SD 95% CL(Ep)
Skew Icos 151.75 79.4S 54.27 4s7.7n 1.1293 ICIO 306.37 93.71 106.13 560.02 0.0599 ICIS 465.92 63.35 256.43 318.73
-1.120
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1C2s 5341 23.66 471.92 612.51 0.4399 3C40 619.19 I5.S4 571.27 659.17 43130 0.8.
3250 658.69 11.70 623.21 68.87
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Page 4 of 5 PpKCICR Test Number: 1.5 Daily Chemistry:
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.PpKCICR Te st Number: I1 Day I
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Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 1.20 1.15 I
I I
I I
I I
I I
I
-State and USEPA Mandated Control Limits Limits Set According to A 2 Standard Deviations 1.10 I 1.05 1.00I 0.95 1 I
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0.8 Laboratory Warning and Control Limits Limits Set According to 10 th and 2 5 h Percentile CVs
- a.
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I Test date 7-day ICs = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
- Central Tendency (mean IC25)
--Warning Limits (mean IC23
- SA, 0 )
.Control Limits (mean IC2. +/- SA 25 or 2 Standard Deviations)
Environmental Testing Solutions, Inc.
Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dwbla
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-t_
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Control Lilits SA.i Warning Limits 2SAh CT.2S CTr.+2S
- 1 CT-SA.
CT + SAJ Test number Test date 7-day ICts cr (iL N&CI)
(WL NaCi) 1 0644-02 1.06 2
0749-02 1.03 1.05 3
02.06402 1.05 1.05 4
09-04-02 1.05 1.05 5
1042I.t02 1.03 1.05 6
1114542 1.03 1.04 7
12-0342 1.02 1.04 S
124342 1.03 1.04 9
124442 1.02 1.04 10 1246402 1.03 1.04 1 1 12-12142 1.04.
1.04 12 12-1t42 1.04 1.04 13 014743 0.96 1.03 14 024443 0.99 1.03 15 034543 1.05 1.03 16 044-083 1.03 1.03 17 054063 1.05 1.03 22 0644403 1.07 1.03 19 07-0S43 1.03 1.03 20 034543 1.04 1.03
- netic WaUer I
Laboratory I
USEPA Control Limits SAM s
Warning Limits CT - SAM CT + SAIS i
CT + SAj i
I~~~~~~~~~~~~~
USEPA Sn Conttol Limits CT -SA CT + S&9 CV 0.02 1.00 1.09 0.02 0.96 0.02 2.02 2.03-.
o0.0 0.97 0.01 1.02 1.03
'.b0 0.97 0.02 1.02 2.02
'MOB 0.96 0.01 1.02 1.07 0.02 0.96 0.02 1.01 1.07 0.03 0.96 0.02 1.01 1.07 0.02 0.96 0.02 1.00 1.07 0.02 0.95 0.02 1.00 1.07 0.02 0.95 0.01 1.01 1.06 0.02 0.95 0.01 1.01 1.06 0.02 0.95 0.02 0.92 1.03 0.02 0.95 0.03 0.92 1.02 0.02 0.95 0.03 0.92 1.03 0.02 0.95 0.03 0.92 2.02 0.03 0.95 0.02 0.92
.1.0 0.03 0.95 0.03 0.92 1.03 0.01 0.95 0.02 0.92 1.03 0.03 0.95 0.02 0.93 2.S0 0.02 0.95 1.13 1.13 1.13 1.13 1.13 1.12 1.12 1.12 1.12 1.12 1.12 1.11 1.11 1.11 1.11 1.11 1.11 1.11 1.11 0.12 0.13 0.12 0.13 0.12 0.12 0.13 0.13 0.13 0.13 0.12 0.23 0.17 0.17 0.17 0.1l 0.13 0.12 0.13 0.27 0.87 0.37 0.27 0.37 0.36 0.26 0.36 0.36 0.36.
0.26 0.26 0.25 0O25 0.25 0.25 0.36 0.26 0.36 1.23 0.47 0.52 1.52 0.65 1.23 0.47 0.52 1.52 0.65 1.23 0.47 0.52 1.52 0.65 1.22 0.47 0.57 1.52-0.65 1.22 0.47 0.57 1.51 0.65 1.22 0.47 0.57 1.5 1 0.64 2.22 0.47 0.57 1.51 0.64 1.21 0.47 0.57 1.50 0.64 1.21 0.47 0.57 1.50 0.64 1.21 0.47 0.57 1.50 0.64 1.21 0.47 0.57 1.50 0.64 1.21 0.46 0.57 1.49 0.64 1.20 0.46 0.57 1.49 0.64 1.20 0.46 0.57 1.49 0.64 1.20 0.46 0.57 1.49 0.64 1.21 0.46 0.57 1.49 0.64 1.21 0.46 0.57 1.50 0.64 1.21 0.46 0.57 1.50 0.64 1.21 0.46 0.57
- 1.50 0.64 0.40 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 0.39 1.70 0.02 1.70 0.01 1.70 0.01 1.69 0.01 1.69 0.01 1.68 0.02 1.62 0.01 1.62 0.02 1.63 0.01 1.62 0.01 1.62 0.01 1.67 0.02 1.66 0.03 1.67 0.03 1.67 0.02 1.67 0.02 1.67 0.02 1.67 0.02 1.67 0.02 Note:
7-d ICa day 25% inhibition concentration. An eatimntion of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT - Central tendency (mean IC2.11 S I Standard deviation of the 1C2s values.
Laboratory Control and Warning Limits Labontory control and wanming limits weeestblished usingthe standard deviationofthe C2, values conesponding tothe lOth and 25thprcentile CVa lhe rnnes ae more stringentthan the control and warning linbtsrecomnended by USEPA for the tes method and endpoint.
Sam.
Standard deviation corresponding to the 10[ percentile CV. (SA,1 - 0.0O)
SAjs - Standard deviation coresponding to the 25s' percentile CV. (SAM - 0.17)
- U.tSEPA Control and Warning Limits SL - Standard devietion corresponding to thc 75d peccentilc CV. (S,7s
- 0.45)
SA@. ' Standard deviation corrtsponding to the 90' percentile CV. (S,%qe - 0.62)
CV - Coefncient orvariation of the iC. vanlu i.
j I
USEPA. 2000. Understanding and Accounting for Method Variability in %Vholc Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA.t33-R40403. US Environmental Protection Agency. Cincinnati, Of1.
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Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water
.~~~~~~~~~~
2s
~...........................................
Test date 0.4 0.2 7-day IC25= 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC 2 )
Laboratory Warning Limits (mean IC2 S,., 0, SA 0=0.08)
Laboratory Control Limits (mean IC25 i SA.25. SA.25 0.1 7)
-USEPA Warning Limits (mean IC25 + S,., 5, SA75= 0.45)
USEPA Control Limits (mean IC2, + SAgO0 SA90 0.62)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test Control Control Mlean number Test date Survival Reproduction for Control Mean for Control p.)
(offispringfenale)
Reproduction
(%)
Reproduction
)
for PVMSD (%)
(offspring/fernale)
CV (%)
1 06-04-02 100 26.0 8.1 3.8 14.7 2
07-09-02 100 29.5 27.8 9.9 9.0 3.5 11.7 13.2 3
08-06-02 100 28.4 28.0 8.0 8.7 2.7 9.5 11.9 4
09-04-02 100 31.4 28.8 10.4 9.1 3.0 9.5 11.3 5
10-08-02 100 31.1 29.3 6.7 8.6 2.9 9.4 10.9 6
11-05-02 100 29.5 29.3 9.2 8.7 2.5 8.4 10.5 7
12-03-02 90 34.0 30.0 8.0 8.6 2.7 8.0 19.2 8
12-03-02 100 33.2 30.4 6.2 8.3 3.3 9.9 10.1 9
12-04-02 100 32.5 30.6 6.0 8.1 3.2 9.8 10.1 10 12-06-02 100 29.7 30.5 11.0 8.4 3.0 10.0 10.1 11 12-11-02 100 33.8 30.8 13.7 8.8 2.9 8.5 9.9 12 12-18-02 100 30.5 30.8 7.4 8.7 2.9 9.4 9.9 13 01-07-03 100 33.2 31.0 7.0 8.6 2.9 8.6 9.8 14 02-04-03 100 32.3 31.1 8.1 8.5 2.7 8.4 9.7 15 03-05-03 100 28.7 30.9 5.1 8.3 3.5 12.1 9.9 16 04-11-03 100 26.3 30.6 6.2 8.2 2.5 9.6 9.8 17 05-06-03 100 27.6 30.5 10.8 8.3 3.2 11.5 9.9 18 06-04-03 100 25.9 30.2 5.9 8.2 2.6 10.1 9.9 19 07-08-03 100 29.0 30.1 11.6 8.4 3.2 10.9 10.0 20 08-05-03 100 33.3 30.3 6.6 8.3 4.7 14.1 10.2 Note:
CV - Coefficiniftovariktion for control reproduction a'emrage, the CV for control reproduction is 83% in Environmental Testing Solutions, Inc. Ceriodtap/ia chronic toxicity tests.
Lower CV bound determined by USEPA(10& pereintile) - S.9%,
Upper CV bound determined by USEPA (904 percentile) - 42%
blSD - Mlinimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.2%
from the control.
Lower PMSD bound determined by USEPA (10 e percentile) - I It%
Upper PMSD bound determined by USEPA (90k percentile) - 37/.
CT - Central Tendancy (Mean Control Reproduction, CV, or PISD)
-The lower and upper bounds were calculated by the USEPA using 393 tests conducted frorn 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R00-403. US Environmental Protection Agency, Cincinnati. OH.
- 0805.03XIs
Environmental Testing Solutions, Inc.
Ceriodaphnia dubia Control Reproduction and Coefficient of Variation in Sodium Chloride Chronic Reference Toxicant Tests 40
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Control Reproduction or Coefficient of Variation (CV)
Central Tendency. (mean Control Reproduction or CV)
Control Limits (mean Control Reproduction or CV *2 Standard Deviations)
Environmental Testing Solutions, Inc.
Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia usini Moderately Hard Synthetic Water 50 45 40 35 a
'9) 30 25 20 15 10 5
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Central Tendency (mean PMSD)
_ Lower and Upper PMSD Bounds Lower PMSD Bound (10 percentile) =11%. Upper PMSD Bound (90d percentile)=-37%
(Lower and upper PMSD bounds were determined by USEPA ror the method and endpoint.)
Environmental Testing Solutions, Inc.
'.Pagelo176 loride Chronic Reference Toxicant Test
'A-821-R-02-013 Method 1002.0)
Species: Ceriodaplznia dubia CdNaCLCR #: _
3 J..
Dilution preparation information: -
Comments:
NaCI CHM number.
HO
__1.__
Stock pmparation:
100 g NaCUI (dissolve 50 gNaCI in 500 ml deionize water)
Dilution (mgL) 600 300 1000 1200
- 1400 Stock volume (mL) 9 12 15 1
21 Diluent volume (mL) 1491 14SS 14S5 1432 1479 Total volume (mL) 1500 1500 1500 1500 1500 Test organism information:
Test information:
l Organism age:
2A. AwLs at5 Randomizing template:
Ie&L~O)
Date and times organisms of-6S Ob Ot~bZ 1tl1O Incubator number and were born between:
shelf location:
Organism source:
c1-2 -lCb PA-F YCT batch:
p(
122*031 Transfer bowl information:
pH = 1,tS Temperature = Z. 0 Selenastrum batch: A z
Daily renewal information:
Day Date Test initiation, Control water batch Analyst renewal, or used
.. ____ ______termination time 2.-
1_
ot-v -6 b iit'iiii Z: 0 l30S-I o
5 t-1G l '
5..
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6 O~.".cm 13O OOt R
.Aa ooWO 0
5 o 6 I -A O Control information:
Acceptance criteria Summary of test endpoints:
% of Male Adults:
S 20%
.7-day LC50 OgO
% Adults having 3P Broods:
2s0%
NOEC P
% Mortality:
7S20%
LOEC JnC L.
Mean OffspringlFemale:
3 15.0 offspringfremale ChV I
% CV:
b.Io'Y'
< 40.0 %
IC25 1
IEnvironmental Testing Solutions, Inc.
Page 2 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:. 13 CONTROL Survival and Reproduction Data Replicate number l
Day 1
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3 1 4 5
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- j I % Mortalitv:
1 70 *'
I Mean OffsrinWFernale:
1533.0 I° 1l% Reduction from Control:-. 0.9 76
a..
. Environmental Testing Solutions, Inc.
Page 3 of 6 4
Species: Ceriodaphnia dubia CdNaCLCR #:.3 800 mg NaCI/L Survival and Reproduction Data Replicate number
_ _ _ _ _ _ _ 1 2 1 3 4
5 6
7
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l 3.
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1000 mg NaCIS Suirvival and Reproduction Data I_____
IReplicate number l
Day 1 1 2
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07 Mean OffspringtFemali
' l 27.9 j
% Reduction from Control: l
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Environmental Testing Solutions, Inc.
Page 4 ofE6 Environmental Testing Solutions, Inc.
Page 4 of 6 Species: Ceriodaphnia dubia CdNaCLCR #:
t5 1200 mg NaCI/L Survival and Reproduction Data Replicate number l
Day 2
3 4
5 6
7 8
9 I
10 I
Young produced O
O 0)70D l
_ Adult mort-ity L-l-
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% Mortality:
107 IMean Offspring/Female:
(I
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% Reduction from Control: l
- l.
.7 1400 mg NaCI/L Survival and Reproduction Data IReplicate number 1Da I
2 3
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7 l
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§ Atdut mortlty L-L
§e L-l l
l 3
Young produced L.
O N
E 6 1 9
10
____j Adultlmortality U. [
U ILAr LTlU I L-Jl l_
l 4 Young produced C),
j 12i i
iO Adult mortality l
jll1 4_
Adult mortality.
l
_lol lLl Young produced
__Af NJ __
7 j
Young produced j
j Ij.j.*
j j
j j& O Totlyoung produced Final Adult Mortality l.
l e
l 1
l L
l iCl Concentration:
I %Moeality:
I l
Mean Offspring/Female:
1 I.
1
% Reduction from Control:
.d.1 7X
Environmental Testing Solutions, Inc.
Verification or Ceriodlaphnula Reproduction Totals Control Day Replcate number Total 2
3 1
m-9 It, I
- 0.
0 0
0 I
0 0
0 0
0~
0 2
0 0
0 10 01010 0t 0 0 0
3 000OI 00 0 10 O.-.
0 4
4 6
4 15 41 3
. 5 5
5 -;5 46 5
12 14 121 12 0
14 13 14 ii13 117 6
0 000 15 0
0 0-L 0
0 Is 7
IF S1 16 15 12 1I 16
-iT IS
-155 Total 34 353 334 29 136 35 34, 33 333 600 mg NaCI/L Day Re Ulcate number
-Total I. 2 3
5 6
7 8
9 I10 1 0 0
0 0
0 0
0 0
0 0
0 2
0 O
0 0
0 0
.0 0 0
.0 0
3 00 0
0 00 0
0 0
0 0
4 S
4 4
6 4
4 S
5 4
5 46 5
16 0
1 2 0
0 IS5 13 14 1 2 114 96 6
0 11 0
3120 0
0 0 0 3
7 1 5 13 1
1 3 16 17k 1 7 15 Total 36 301 30 3
33 13 33 3
800 mg NaCI/L_______
Day
~~Repikeat number ruta 1
0 0
0 0
0 0
0 0I 0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
4 5
5 S
4 6
4 4
4 3
44 5
12 0
1 0
0 12 13 11 12 14 75 6
0 14 12 12 13 0
0' 0
0 0
51 7.1 14 1
L4 34 17 17 14 152 Tota 311 331 3 31 3334 413_331 322 1000 ma NaCIJL I
Day Il~~~e Ilicate number Tota Day 3T4 W
T" 7
It Total I
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 3
0 0
0 0
0 0
0 0
0 0
0 4
S 3
4 5 5
4 4
6 3 5 44 5
0 0
0 12 0
0 0
0 13 14 39 6
14 1 2 13
- 0.
10 12
- 10.
13 0
0 84 7
1 1 II 6
10 15 10 14 12 10 112 Total 30 26 2
30 25 31 241 33 28 29 279 1200 mg! NaCI/L Day
-j- -2 1 5
6 7
8 9
1 Total 2
0 0
0 0
0 0
- 0. 0 0
0 0
3 0
0 0 0 0
0 0:
0 0
0 0
4 2
3 I
3 0
4 3~ 0 2
0 ISa 5
0 0
0 0
0 3
0, 0
0 0
3 6
12 a 2
7 0
0 3-7 4
0 43 7
12 1 1010o 0 0
0~ 1 2
54 fotal 26 22 3
20 0
7 6
is 1
2 18 1400 mg NaCI/L 1
Da
~~Replcate numberToa y 1 2
3 41 5 -
7 81 9
10-Toa 1
0 0
0 0
0 0
0 0
0 0
0 2
0 0
0 0
0 0
0 0
0 0
0 4
0 2
0 2
2 4
2 0
0 0
12 4
0 0
0 0
I 0
0 9
6 5
3 3
2 4
0 3
0 0
0 20 7
0 0
3 0
2
.4 9
4 3
0 30 fToal 9
5 6
4 8
9 1
1 0
Environmental Testing Solutions, Inc.
Chronic Wholc Eucnt Toxicity Test (EPA-821-1R02-013, Method 1002.0)
,. S,>>
Species: Ceriodaphnia dubia
'1 5Quality Control Vcrification of Data Entry, Calculations, and Statistical Analyses Test number.
CdNsCICR N42 Test dates:
August S-12,2003
.Ravwiwed by-. I1'UA Concentration
__Repicat number Survival Avetage reproduction comd..t at rft...- redurdtes trow (mg/L NaC) 1 2
3 4
S 6
7 a
9 10 (V.)
(offspring/lemale)
Variation CX 1
(%
Control 34 35 30 33 34 29 36 35 34 33 100 33.3 6.6 Not applicable 600 36 30 30 32 33 34 31 35 33 36 100 33.0
!69 0.9 800 31 33 34 31 30 32 34 33 33 31 10 322 i
4.3 3.3 lo"0 30 26 23 30 25 31 24 33 28 29 100 27.9 11.8 16.2 1200 26 22 3
20 0
7 6
1S 14 2
100 11.8 79.2 64.6 1400 9
5 6
4 a
9 14 5
11 0
90 7.1 5+/-7 78.7 Dunnelt's MSD value:
PMSD:
4.68 14.1 MSD -
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure attest preciuion. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whale emuent toxicity tet. On average, a significant difference occurs for Environmental Testing Solutions. Inc. chronic toxicity teats when a toxicant reduces Ceriodaphnia reproduction by 10.2% from the control.
Lower PMSD bound determined by USEPA (10d percentile) - 11%
Upper PMSD bound detennined by USI1PA (90* percentile) - 37%
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratoris for Cerbaphnla reproduction in chronic reference toxicant testr USEPA. 2000. Understanding and Accounting for Method Variability ir Whole mumnt Toxicity Applications Under the National Pollutant Discharge Elimination Progm. EPA-g33-R-00-003. US Cnvironmental Protection Agency, Cincinnati OIL0I
Environmental Testing Solutions, Inc.
Statistical Analyses Cerlodaphla Sarvlval and Reproductlon Tat-Roproducctle Szrt Due:
/C3/3 Tat D Cd&aCCR "plal:D REF-RatfTxizcnt E
Dadte: V/203 Lab ID
. ETErm Taez Solztiaon S mbTpe NACL-Sodwu che pe Date Prcicol:
CNRONlC<(EPA421-R42403)
Tst Speces:
CD.Ceiodaphnia dubi.
Comments:
Cone-Y 1
2 3
4 5
6 7
S 9
1o DWcontml 34.000 33.000 30.000 33.000 34000 29.000 36.000 33.000 34.000 33.000 600 36.000 30.000 30.000 32.000 33.000 34.000 3100o 3S 000 33.000 346000 60 31.000 33000 34000 31010 30000 32.000 34.000 33000 33000 31.000 1000 30.000 24000 23000 30000 25000 31.000 24.000 33000 2t.000 29.000 1200 26.000 22.000 3.000 20n000 0.oco 7.000 o
-0 o0o 0
14000 2.000 1400 9.000 3000 64000 4,000
&.00 9000 14.000 t
l000 C1100 0.0C0 Transform: U.tbansdermed tank
&-TsIIed beotookc Cenc-ve MAot N-Mean Mta Mn l Max CVV.
N Sua Critlcal Mean N-Mean D-Canot 300 1A000 3300 29.000 36.000 6.d47 10 33.303 1.0000 600 33.000 0.9910 33.000 30.000 316.000 6.31 10 100.50 75.00 33.000 0.9910 3O 32.200 0.9670 32.200 30.000 34.000 4.343 10 34.50 73.00 32.200 0.9670 1000 27.900 0.3373 27.900 23.000 33.000 11.761 10 63.50 73.00 27.900 0.5373
- 1200 11300 0.3544 11.300 0.000 26.000 79.177 10 55.00 75.00 11.S00 0.3544
- 1400 7.100 0.2132 7.100 0.000 14.000 55.72
-- 10
--- 53.00
75.00 -
7.103 --0.2132 Aalfllbar Tests Statistic CALIC21 Skew Kurt Knbow mv D Tast Wcates nomal distrbution (p. 0.01) 0.97533444 1.033 0.2161160 2.02911317 Baulctrj Test idiaeas unequal variances (p - t9fE
)
41.099598 15.0863171 Hnntbes kTest( lO.0S)
NOEC LOEC CkV TU Stee WMany-One Rank Tat S0O 1000 994.427191 0.125 Traunents vs O-Controd Unear Interpolation (200 Reuampies)
Point SD 95% CL Skew tCos 826.27907 79.4249321 533.365739 873.421007
-2.3310 tC1o 903.72093 37.9833404 342.U3S39 997.016043 0.6377 ICt5 9S1.162791 33.737917.912.343214 O11L65158 4.2947 1.0 1C20 1015.65217 13.6163229 96.336416 1040.6356:
4.9535 Q9 IC2s 1036.3154 14.163d132 1009.57693 1064.4343 0.ll22 IC40 1098.38509 21.0037292 1070.16427 1154.35428 L.0O0S 0.° IC50 139.75133 27.9547482 1102.23977 1214.10309 1.1163 0.
0.0
&0.4 0.3 0.2
.i.
/
e 5
3 0
1000 1500 cose Y%
P Oes.-P..p ns. Fist 40 35.
30.-
o 25-j 20 IL 15 to 0d-O5.03ds
Environmental Testing Solutions, Inc.
Statistical Analyses Used bor PtSD eaculation only.
Start Date:
V5103 End Date:
93(12 Sample Date TesttD:
C Lab ID:
I ProtocoL C
Ceriodaphana Surviwi and Reproduction Test-Reproduction CdNaCtCR Sample ED
.TS-Enr. Terstin Sotuieonx Sampl Type:
HRONIC-(EPA-21.R02-13)
Test Species:
REF-Ref Toxicant NACL-Sodlum dleoddc CD.Cedodaphnia dueia Conc.%
1 2
3 4
S 8
7
£ 9
to D-Coutrol 34.000 35.000 30 000 33.000 34.000 29.000 34.000 35.000 34.000 33.000 600 36.000 30.000 30.000 32.000 33.000 34.000 31.000 35.000 33.000 36.000 300 31.000 33.000 34.000 31.000 30.000 32.000 34.000 33.000 33.000 31000 1000 30.000 26.000 23.000 30.000 25.000 31.000 24.000 33.000 22.000 29.000 1200 24.000 22.000 3.000 20.000 0.000 7.000 6.000 1.3000 14.000 2.000 1400 9.000 5.000 6000 4.000
.000 9.000 14.000 l000 11.000 0000 Transform: Untransaormned l-Tailed Conc-%
Mean N-Mean Mean Mln bMax CV%
N "Stat Critical MSD D-crntrol 33.300 1.0000 33.300 29.000 36000 6647 to 600 33.000 0.9910 33.000 30.000 36.000 4331 10 0.147 2.237 4.680
- 00 32.200 0.9670 32.200 30.000 34.000 4.343 10 0.537 2.287 4.680
- 1000 27.900 0.8371 27.900 23000 33.000 11.761 10 L638 2.287 4.680
'12CO 11.t00 0.3544 11.00 0.000 26.000-79.177 10 10.504 2.2t7 4.680
- 1400 7.100 0.2t32 7.100 0.000 14.000 55.728 10 M2ot1 2.2t7 46680 Auxiliary Tests Statistic Critical Skew Kurt Itolmogotov D Teat indicates nonoga ditribution (p > 0.01).
0.97553444 1.03S 0.21611605 2.0;911317 BaieasTest indicates uiqutat Veklwsoci(p *1.96E.08) 41.0995598 15.0863171 Hypothesis Test (-all, 0.05$
NOEC' LOEC ChY TU MSDu MSDp MSB USE F-Prob di DunetfsTest SOO 1000 S94.427191 0.25 4.6802761 0.140548S3 13CS.21667 20.9462963 1.IE-21 5.54 Treatments vs DControl 0380.-03.Xb
t.
'.Sin' r
^
7
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i Environmental Testing Solutions, Inc.
- Page 5 of 6 Species: Ceriodaphnia dubia CdLNaCLCR #:
\\3 Daily Chemistry:
Concentration Parameter 0
~~pH (S.U.)
-. *.1
~
3
- lZ 9
DO (mg/L)
- IR.3 l'
A CONTROL Conductivity 2 t 41
~~(Rlmhos/crn) 7_
31 31_
Alkalinity 5 S\\l
.. (mg C~aCO3/L) -.
_\\
Hardness 6
\\
Temperature.
(°C)
Z5 9?
Z.
Z, S
4t pH (S.U.)
%8 stlS
- 1tt0
~;40 DO (mgtL) 8 B=
600 mg NaCI/L Conductivity (jimhos/cm) q 9
L %1IA Temperature t°C)
Z<.A z9-S5<
\\
[u.
pH (S.U.)
- Q. E3 -l
-. LV oq*Et I le DO (mglL)
.t 8
- 8.
800 mg NaCU1L Conductivity (gmnhos/cm)
+ i 43 Temperature Zw o i
(CC)
ZS. I 53 5
/,sE:PH.U.)
-4.
I q- (OR w-4 0
-494
-- (g S4 o-'
O00 mg NaCVJt Conductivity.li
~~(tmhos/cm) -%L -lt -
Temperature 2
At Z
~
(°C)
?-S
- 25-. 3 V.
T-pH (S.U.)
-+8(
?4.615
-4.L
.(v 4-to I. ::po DO (mg/L)
,o
- iX° 1200 mg NaCU1L Conducm)%+,
e-ti3ity Temperature Z
z
° Ze e 5
- 2.
l*
pH (S.U.)
A.t tb
.C iS:t
-9
.DO (mg1L)
[38- >f ji.ts*XE.
1400 mg NaCI/L Conductivity !5 2t6 34 Temperature
- 7.
25.3 2Lq.
2S. I A
Initial Final Initial Final Initial Final Initial Final iP
,E-
Environmental Testing Solutions, Inc.
I' Pag-e 6 of 6 CdNaCLCR #: 135 Soncentration lParameter 3
5l 6
^-
~~~~pH (S. U.)
l,.S3
.S o
.fi
.34
'q.5
§
~~~DO (mglL)
- -rs LONTROL Conductivity 5'1 33303Z
~~~~Alkalinity lO\\\\
(mg CaCO3/L) w
~~~~Temperature
-ZS,
Qqt
?S,,Z.1-
- 2.
- e S.
~~pH (S.U.)
o Q
- .oowto6 4(2
.96 2,(
5-i s
~~~~DO (mglL)'
A;ww s >X-b4.
b 00 mg NaCVIL
_C(onductiIvity$
1451 Temperatu re Z
2 3 Zt4
.3.Z.
X
~~~~pH (S.U.)
'q-4eq 2
b8W7
.i
.S q9
-a
~~~DO (mg/L) s>
3*
7 t
+6 f
i00 mg NaC11L Conductivity i
X l2Z
=
~~~Temperatu re S*
Z.
t.oZ53 2
X.1 1000mg~~~aCUL
(
LlmS/c18 ZS ZIA; Z5.5
- 24. sL C
~~~~~pH (S.U.)
-q A'b
=4q l
3 t X
.loS.,G.?
i200 mg NaCltL Co-nduc-tivity st-2(5.tZ5o
~~~Tempierature Z
- 4. 5tZll.3.. 5 S
~~~~pH (S. U.)
1ASlaB 7t
- .X as
~q
~blRt 1400 mg NaCU/L Conductivity.z 4
Y
^
2X5 t6 Ze/
~~Temperaturce
.l 1
Z.Z 2~o Z.
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Location _AMILN COCUY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
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PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO.
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whon merup the "on or ths persona directly responsible forgetharing the Acting Environmental Supervisor 423 843-700 03 09 10 Site Vice President
~infonation, the Information suibmitted Is. to the best of my knowledg enmd belief, true, 423__843__6700__03__09___
Site \\flce President
- t. and complete, I am aware.I tht thre e signficant penalties for submittig false SIGNATURE OF PRINCIPAL EXECUTIVE ifltonIon, Including the possbility of fine id dinpulsoent for knowing viol0thons.
OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED
._._._CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference afl atachments here)
EPA~~ Fom32-RV39)
Peiu dtosmyLeue ae1o EPA Form 3320-1 (REV 3199) -
Previous edition$ may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Inclide FaciltV Naeocatln fDifferent Name TVA - SEQUOYAH NUCLEAR PLANT -
.Address P_.Q Q0 2000
_ _ {INEROfflqE %gN-2_
SODDY - DAISY T 37384_
fadffy__DLA-_gE QUOAHNC A
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Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESj MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
OMB No. 2040-0004 1FTN7026450 7
N103 G F - FINAL PERMIT NUMBER DISCHRGE NUMBER LOW VOL. WASTE TREATMENT POND I
MONITORING PERIOD I
EFFLUENT YEAR MO DAY R
MO DAY From 03 T08 1 01 I To 03 l 08 l 31l NO DISCHARGE [I] -'
NOTE: Read Instructions before completing this fbom.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
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.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~tl SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penally of low ViW h docment and atacents were pped der my I
TELEPHONE DATE raction or supervision in accordance with a system designed to as thnt qualified peEsoHnN DATE Richard T. Purcell p
8ro no and s t tine Worn aion tbmitted. Based on my Wdiun of rt pe m
r Am, or' I peons r clyresponsiblfr g Acting Environmental Supervisor 423 843-700 03 09 10 S~~te Vice President tieV Information submitted Is.* to the besl of my knowiedge end belief. bun.
423__843__6700_03__09____
Site Vice President scorand ncoplete.
I am awm tn tee e sIgnfic penalties for submittIng false SIGNATURE OF PRINCIPAL EXECUTIVE I
TYPED OR PRINTED
.orm_
ation includnig Xt possibility of l'me and sonent for k
violati.
OFFICER OR AUTHORIZED AGENT DNUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referemnce BYt attachments here)
Turbine Building Sump was aligned to the Yard Pond on 8/28/03 @ 0730 and realigned to the LVWTP on 8/29/03 @ 1010.
EPA Form 3320-1 (REV 3199)
, Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facilitv Nan.octon fDifferen)
Name _
VA - SEQUOYAH NUCLEAR PLANT Addres
.Ps _ __
_ _&ff ROfflQE I&M SOODY-DAISY TN 37384 faj&__MA - SEQUOYAH NUCLEARA PLNT Locaton _AMILTN CO UNTY..........
ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN0026450 l
107G F-FINAL PERMIT NUMBER I DISCHARGE NUMBER METAL CLEANING WASTE POND I
MONITORING PERIOD l
EFFLUENT Form Approved.
OMB No. 2040-0004 F Iom l9 &mO I DAYTo i
EAjMjA From 103 l 08 1 01 ITo 03 l 08 l 311 NO DISCHARGE F
NOTE: Read Instructions before completing this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
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~ D~YM NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Codlify under Penialty of low that twos docurront and all ettadvnents ware prepared unider MY TLPOEDT d irection or supen~sion in accordhnce with a system designe to assure thast qualified peronnl TLEPONEDAT Richard T. Purcell nrod evakiate On Information submjtled. Based on my inquliy of the person or pro who mansg te system, or those persons directly responsile for gatherhSg e e~s t
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n Intmelion $LtMiKd It, to ft beg of my knwldge and beliet,.IIWo, Acm niomna uevsr 423 843-6700 03 09 10 Site Vice President accrate. and complete. I unaware tyt there are s bIniflcart Penalties for subitAting OF SIGNATURE OF PRINCIPAL EXECUTIVE I
TYPED___________
foutcnORddlg h PUPRINeTEDl riwt~kfO~i tI~ifl.
I OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VOLATIONS (Rerence all aachments here)
No phosphorous bearing cleaning solutions were used.
EPA FonTi 3320-1 (REV 3199)
Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS flncdude Faclity Nanocaton ifDfhfrent Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. Bx
-2000
-_~ ~~~
- -NEOFC
-2 SODDY-MDAISY_ TN 37384 ZaLejlt A A-SEQUOYAH NUCLEAR PLAN T Locat n AMILTN OU_._
ff...
ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (OR)
(SUBR 01)
I TN0026450
- l. l 110G17F - FINAL I
PERMIT NUMBER I DISCHARGE NUMBERl RECYCLED COOLING WATER Form Approved.
OMB No. 2040-0004 r
l MONmITOnRING-PERIODl I EFFLUENT In
~~~~~~~~~~~~~-1 From 03 l'
08 f
To 03 08 3
NO DISCHARGE [
NOTE: Read instructions before conpleeina this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 040 1 0
0 7PWT; 7 77ee>~i6O
<K Su
~7IL EFFLUENT GROSS VALUE T
9IMU T
M m
SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT le 0530 1
0 0
'7 7MT7
~-
77~i mG/I..
EFFLUENT GROSS VALUE
-U DAiLYM.
x OIL AND GREASE SAMPLE
- 1*9*
MEASUREMENT 19 0556 1
0 0
MG/
Y EFFLUENT GROSS VALUE RQIEE M
SAMPLE 19 MEASUREMENT 01042 1
0 0
fr.~MT~,
~A~**
<e~
7 I~W~MG/L.
~A(i~CMO EFFLUENT GROSS VALUE QL MIt 7/
SAMPLE 19*****
- 1 MEASUREMENT 01045 1
0 0
7TPR17h.7
- i**77~-.
- ~~U MG/L
~
~I~'~
MpOS_
EFFLUENT GROSS VALUE DA/L M F,_
PHOSPHORUS, TOTAL (AS P)
SAMPLE 1
MEASUREMENT 1
00685 1
0 0
'R I~
'i~~
m*****?~'
MG/L.
DMPO FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1
0
- 0.
mo Z
OD-LC TfY EFFLUENT GROSS VALUE MO' NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Cef under penalty of law that is docm aid adl aftewnents weis prepwed under my TELEPHONE DATE directon o supentision in soardence wIt msynem died to assure VW qualified personnel
(
Richard T. Purcell piopaity gahr and ealuat the infmation submitted Based on my quiry of th perso or Persons whio manee the system, or ftho persons directly responsible for ga19 theig
~
Atn niomna uevsr 430 9
1
,~"mf rInkh on submiKd is to4 f boofnt d myci and belief~m, tre Acting Environmental Supervisor 423 843-6700 03 09 10 Site Vice President acate, dnd r'ltU 1
m n t
I penalties for
___llng fals SIGNATURE OF PRINCIPAL EXECUTIVE I
380 TYPED_____________________
ORfo
- PNDon, Incudig the possibility d fine and linprisonment for knowing violions OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED
~efmfrJCODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all atachments here)
No Discharge this Period EPA Form 3320-1 (REV 3199)
Previous eftons may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Indude FadlltyName ocetln IfDlirent)
Name _ _A
- SEQUOYAH NUCLEAR PLANT Address P.Q.BX 2000 g0Yko _j
_L8
..RFC
.SS.
fadjV. _TVA.SEQUOYAH NUCLE7A PLA-N Loraon.
HAMILN O_CQU TY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MWOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
Ii
~TN0026450
[I 116.
F -FINAL PERMIT NUMBER
[DISCHARGE NU BACKWASH Form Approved.
OMS No. 2040-0004 l
1 MONITORINGr PERIOD I
EFFLUENT o AT MO I DA From 03 l 08 l 01 1 T 03 108l3
' NO DISCHARGE El -
NOTE: Read instructions before cornpleting this form.
PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE 0
9A O
1 31 VISUAL MEASUREMENT O*
01345 1
0 0
1 -
~ -7 7
£iW**
iPSSV U
EFFLUENT GROSS VALUE OIL AND GREASE VISUAL SAMPLE 0
.)
1/31 VISUAL MEASUREMENT 8066 1
0 0
RT.Y YESI
~
7--7.SE VISUAL.
EFFLUENT GROSS VU VALE 11--, W m A~
SAMPLE MOOA NO0 MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT JY PERMIT 7
j7.
7.,
7 SAMPLE MEASUREMENT REOUIRMET SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cetify under penalty of law that this doanetd s alt ttarts were prepared under my TELEPHONE DATE direcion or sprvision ao wi a sytem desed tosss t qua*ifd perslI Richard T. Purcell ro e ter and evalute Oh Information ainemltsd Based on my Inquiry of thr person or Sson the ronn ynmted Ir two trh dsrbtoy responsible for bllet.
ftue
- o I
Site Moo Presidenterson Informationt siirted Ior thos tpeWM 1' renly esponsledg enl gallern thie, Acting Environmental Supervisor 423 543-8700 03 09 10 sacirate, aid cornplete. I an swere tha ther we soffIIati penartie for sumn fas SIGNATURE OF PRINCIPAL EXECUTIVEI TYPED OR PRINTEDio rvOwposiiltyof fine mid knri~sornmert for know~ng violations.
I OFFICER OR AUTHORIZED AGENT AEINUMBER YEAR MO DAY TYPED OR PRINTED CODE EtW K
INAUEFmW WI 2
33670YA O
1 COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments hee)
Operations performs visual inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page I of I
PERMITTEE NAME/ADDRESS
(/nclude Faciit NameaoatIon fDfflerent Narn __VA - SEQUOYA_ NUCLEAR PLANT ddreu P.O. BQX 200 _
_ ___Q
~
.D._AISY_ TN 37384_
feajtyTVA -SEgQUjAH N (LEAR PAN T____
a oHAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
I TN0065 l
117 G F - FINAL PERMIT.NUMBER I
DISCHARGE NUMBER BACKWASH 1 _ _
NILqRING EERIOp l
EFFLUENT Form Approved.
OMB No. 2040-0004 YEAR I MO I DY From 03 l08 l01 I To 03 708 731 1 NO DISCHARGE E
NOTE: Read Instructions before compietina this form.
I NAMEMFTE PRINCIPAL EXECUTIVE OFFICER I certify uider penatY of law VW tSis docuent aO1d alt attedmet wern prepared uwd my TELEPHONE DATE drection or supervision hI motdance with a system desind to am" tat qua8ied personel h4w tD\\L,-n Richard T. Purcell Proper gathe and evaluate the ft.,iad son slted Based aon my Inquiry of t person or A
StViePede pens who aft systm or nc proni dirctl Acting Ernvironmental Supervisor 423 843-8700 0
infoVPation. fhe infoantlon submitted is, to On best of my knoWg and belief, true.
ActgEno 423 8436700 03 09 10 Site V~ce President accurte, and c"snpelm. I an aw. tt thee e signitcam penalties for submitting h SIGNATURE OF PRINCIPAL EXECUTIVE Wifornsoon, Iliuding the posraililty d f and isoEmet for knowft vicletlons.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY ViOLAlnONS (Refelence eflattchments here)
Operations performs visual Inspections for floating debris and oil and grease during all backwashes.
EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (include FClit NamioctIon if Diffeent)
Name TVA - SEQUOYAH NUCLEAR PLANT Addres P.Q(X 2000 SODYY-DAISYL T 3384 fdg_
J VA-_SEUQUYAH NUCLEAR PANT_
Location HAMILTON _CQUNT
____L ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
MAJOR No. 2040o0004 (SUBR 01) 0MB No. 2040-000 l
TNOO 118 G I F - FINAL I
PERMIT NUMBER l llDISCHARGE NUMBER1 WASTEWATER & STORM WATER I
CAUITet0IKft1 PF=lrln I
EFFLUENT I
FERom M~~jjjOl To D~YA Y
MO o
NO DISCHARGE [M]E NOTE: Read Insructions be" completing this oirn.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE
_ _ _ _ _ _ _ _ _ _ _E X
O F TY PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT 00300 1 0 0
<6RP~~~~~i~rT>
- a*****
'~~~~~~****r,
.2 0'
MG/L 7f W1V -1 EFFLUENT GROSS VALUE M m
WE SOLIDS, TOTAL SUSPENDED SAMPLE 1*
MEASUREMENT 19 0530 1
0 0
REQJTRMET>,
7774 7
i7*-':
2 *1.
A "0o>-
MG/L C"
~tE EFFLUENT GROSS VALUE 3,
_.AILY M
m SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT
.*25 00545 1
0 0
pR~2~.a.
MLJLOCEI RA EFFLUENT GROSS VALUE MU L
S m
FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENTME3 050051 00 P<C0 RTO'-
MGD yJE4TIA EFFLUENT GROSS VALUE M
G DA X
m SAMPLE MEASUREMENT SAMPLE MEASUREMENT
~~~~~~~~~~~~~~~~~~~~~~~7 SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER @I Cw* under Pely of law VWat 11s docwnent and anl atanents ware prepared under my TELEPHONE DATE dion or sion inl aordanc wth asystem dIged b
toasrttqualfled perat
'LX s
4VW a.A8 Richard T. Purcell propety gathe and evalute th rmtIon sttmed. uased on nmykuy o thperson or paraon who menage the system. or those peasr. directly responsible for gateing th Acting Environmental Supervisor 423 843-700 03 09 10 Site Vice President infoWmsnion.
Ite Information suNfIted is, to the beat of my knowledge and bes1e, true.
OF PRINCIPAL EXECUTIVE accurate. end corriplete. I arn aware tha fthre we signifloant penallies for submitting false IATROFPNCALECUVI TYPED OR PRINTEDinfrmi inclualng X possibility of fins and hirprisrnent hor itnowirg viotiotw OFFICER OR AUTHORIZED AGENT CODEA NUMBER YEAR MO DAY TYPED OR PRINTED I
_________________________I_______________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refeence all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.
EPA For 3..-
(RE 3/9
.voseiin a eue ae1o EPA Form 3320-1 (REV 3199)
Previous editions may be used Page I of 1