Text

Notice of Noncompliance with NPDES Permit No. TN0020168, Description of Noncompliance Through Daily Chemistry Data - Ceriodaphnia 7-day Chronic
	ML031480129
Person / Time
Site:	Watts Bar
Issue date:	04/30/2003
From:	Crawford R; Tennessee Valley Authority
To:	; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	TN0020168
	Download: ML031480129 (86)
	v • d • e

{{#Wiki_filter:a:~~~~~~~ -SdS F I0Xi wSl\\s>Vcsp -i;< ? X sSt3; -/;t ft NOTICE OF NONCOMPLIANCE WITH EFFLUENT LIMITATION NPDES PERMIT TN0020168 WATTS BAR NUCLEAR PLANT April 18, 2003 Outfall Serial Number (OSN) 112: Runoff Holding Pond Description of the Noncompliance: Semi-annual toxicity testing of OSN 112 demonstrated chronic toxicity for larval fathead minnows (Pimephales promelas). Sampling was conducted from April 13-18, 2003. The IC25 value resulted in 82.5%, which did not meet the permit limit of Ž100%. There were no toxic effects seen in Daphnids (Ceriodaphnia dubia). Cause and Period of the Noncompliance: The noncompliance is known to have begun April 13, 2003, when the initial test sampling began. Preliminary results from repeat sampling conducted from April 28-May 2 indicates the period of noncompliance likely ended May 2. Final reviews and Engineering confirmation of the repeat test will be completed in late May. The cause of the Noncompliance was due to naturally occurring pathogens. Steps Taken To Reduce. Eliminate, and Prevent Recurrence of the Noncompliance: Corrective actions include the retest. UV pretreatment controls tested in parallel for this outfall indicated no chronic toxicity for larval fathead minnows. This is similar to the Notice of Noncompliance that was submitted for the same outfall on April 21, 2001. A study was performed in 2001 at WBN and indicated that the failure of Whole Effluent Toxicity testing for fathead minnows was due to naturally occurring pathogens in this outfall. The same pattern of failure is seen here. A copy of this study has been provided to TDEC. Additional copies are available upon request. No further actions are believed necessary at this time.

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PERMITTEE NAME/ADDRESS (Inciude FacililYName/ocation if Different) Nomts,_ TVA - WATTS BAR NUCLEAR PLANT Address 2R X _Ltjr-- M -- -- nC -Q -- _obrt J. C E Envro me ta. e _ Ang on_on Attn: Robert J. Crawford, Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES) MWOR For DISCHARGE MONITORING REPORT (DMR) SUBR 01 Om lI7 TNO020168 7 _l l 101 T l F-FINAL I PERMIT NUIMBRP i DSGHAIR; NUMBER BIOMONITORING FOR OUTFALL 101 ALANIT(%DIKIr DDICNn WAr M I F 0 fl I 0 3 l I From 03 104 l Ot T 03 l 04 30_I m Approved. B No. 204D-0004 I l-FLUENT

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PERMITTEE NAMEIADDRESS QInclude Facility NameAlocation if Different) N gyt TVA -WATTS BAR NUCLEAR PLANT f _CIt _M _-WHWAR.WL8E_A1I.. _ _ lq_RE&9QWNY - - -h21 - - Attn: Robert J. Crawford, Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) SUBR SUBRO01 I TN0020168 I I DIDNAIT Nil IRAMD Form Apprved. OMB No. 2040-0004 102 G F - FINAL I I DISCHAPRF NIMRI I YD HLDING POND EMERG OVERFLW WEIR M(NIT(YRIN( PIlC I F FAr MO I AV YAR M D From 3 04 01 To[ 3 l04 30 I EFFLUENT NO DISCHARGE [,j - NOTE: Read instructions before comoletino this form. PARAMETER QUANTIMY OR LOADING QUALfTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNrTS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE (04) CENTIGRADE MEASUREMENT (04) 0010 I 0 0 PERMrT -40 DEG. C. DIY;', ;:-.GRABr~ EFFLUENT GROSS VALUE REQUIREMENT J DAILY MX ' ;jf;C. PH SAMPLE MEASUREMENT (12) 00400 1 0 0 PERMIT 6.0 9 Su WEEKLY J7GRABM, EFFLUENT GROSS VALUE REQUIREMENT MiNiMUM-MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT (19) 00530 1 0 0 PERMIT 30 .100 MG/L 1 DGRAB¶T 30 -.,:I0:.,: MG/L WEEKLyi EFFLUENT GROSS VALUE REQUIREMENT MO AVG DI MX,_,_j_4,; OIL AND GREASE SAMPLE MEASUREMENT (19) 00556 1 0 0 PERMIT . 15 '. 20 MG/L WEEKLY, k,GRAB:> REQUIREMENT EFFLUENT GROSS VALUE REQ UIEMEN MO AVG DAiLY MX FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 PERMIT REPORT REPORT MGD -DAILY )I NSTAN EFFWENT GROSS VALUE REQUIREMENT MO AVG DALY MX F v'i i CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT (19) 50060 1 0 0 PERMIT

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MINIMUM MAXiMUM .y.i SOLIDS, TOTAL SUSPENDED SAMPLE 22 37 (26) 6 12 0 4130 GRAB MEASUREMENT (19) 00530 1 0 0 PERMIT 250 834 LBS/DAY 30 100 MG/ L WEEKLY '; GRAB"' REQUIREMENT EFFLUENT GROSS VALUE R E QIREEN MO AVG DAILY MX MO AVG DAILY MX OIL AND GREASE SAMPLE <21 <32 (26) <5 <5 (9) 0 4 /30 GRAB MEASUREMENT (19. 00556 1 0 0 PERMIT 125 167 LBS/DAY 15 20 MGI'- WEEKLY.' sGRAB-., EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX MO AVG DAILY MX ____,,,j,,_ FLOW, IN CONDUIT OR THRU SAMPLE 0.626 0.862 '03' 0 14/30 RCORDR TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 PERMIT REPORT REPORT MGD CONTIN: RCORDR REQUIREMENT MOAG ALYM EFFLUENT GROSS VALUE R E QUIN MO AVG DAILY MX. SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penatty of law that this documernt and all attachments were prepared under my TELEPHONE DATE .irection or supervision in acordance with a system designed to essure that qualified personnel TELEH7NE W. R. Lagergren properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persmons diredy responsible for gathering the information, the Information submitted Is. to the best of my knowledge and belief, true, eurat( 423 365-8767 03 05 15 SITE VICE PRESIDENT and complete. I am aware that there era significant penalties for submitting false Wormatlion, SIGNATU OF PNCIP L EXEC T inckuding the possibility of fine and inprisonment for knoving violations, OFRCER OR AUTHO?iD AGENT AEA YEAR MO DAY TYfPED OI? PRINTED .FCE ORr _UHR GN RA UBR YM M A COMMENTS AND EXPLANATION OF ANY VIOLATIONS Reference af attachments here) Discharged Low Volume Waste Treatment Pond 14 days in April. NT Re Istruct I I o NOTE: Read nstrucions before compdetinn this orrn. F:0 F 111n.t RFV 391 Pevious editions may be used Page of

PERMITTEE NAMEtADDRESS (Include FacilitrNarelocation ifDferent) Nrnf-TVA - WATTS BAR NUCLEAR PLANT F& U nNT E -)E_ jqQtlon_IHE&QVNay_.. Attn: Robert J. Crawford. Environmentol Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR. DISCHARGEMONITORING REPORT (DMR) SUBR01 I TN0020168 I FDRAIT Nl IR. I I I Form Approved. OMB No. 2040-0004 107 G I F-FINAL I I DISCHARcGr NUMBER I METAL CLEANING WASTE POND IYrALI M I Al MO I From 03 04 01 I To 03 04 EFFLUENT ^** NO DISCHARGE Z NOTE: Read Instructions before completing this form. PARAMETER QUANITY OR LOADING' QUALITY OR CONCENTRATION NO FREQUENCY SAMPLE ~~~ ~~~~~~~~~~~~~~~~~E OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNrrS ANAI PH MEASUREMENT 8.7 8.7 (12) 0 1 / 30 GRAB 00400 1 0 0 PERMIT e****

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PERMITTEE NAME/ADDRESS nclude Facility NameALocation ifDifferent) Nor" TVA -WATTS BAR NUCLEAR PLANT__ Addrets _p Q Jal -M-- _ tY R e . Caw fRd. E Ir nL _e-- Q=fthn-RHE-QWNr - - - - Attn: Robert J. Crawford, Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) I TN0020168 I PERMIT NUMBER Forn Approved. OMB No. 2040-4004 I 111 G F-FINAL I!i,HAR;EI NIMBER COMBINED SEWAGE TREATMENT PLANTS MrNIITODINr( PIl12 IVFAQ MO I DAVI IV FAl I From 03 04 01 I 1 EFFLUENT To 03 MO1-l NO DISCHARGE 0 *-- NOTE: Read nstrudions before comoletina his forn. PARAMETER QUANTFIY OR LOADING QUALY OR CONCENTRATION NO. FREQUENCY SAMPLE ~~~ ~~~~~~~~~~~~~~~~EX OFTYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALSI BOD, 5-DAY (20 DEG. C) SAMPLE <2 <2 (19) 0 5 / 30 GRAB MEASUREMENT 00310 1 0 0 PERMIT 30 45 MGL WEEKLY,:GRAB)'p EFFLUENT GROSS VALUE EQUIREMENT M AVG DAILY MX SOLIDS, TOTAL SUSPENDED SAMPLE 4 12 (19) 0 5/30 GRAB MEASUREMENT 00530 1 0 0 PERMIT 30 45 MG/L WEEKLY -;GRABV EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX '7'8 SOLIDS, SETTLEABLE SAMPLE <0.1 (25) 22 /30 GRAB MEASUREMENT 00545 1 0 0 PERMIT 1.0 MLL TWICEV... GRAB EFFLUENT GROSS VALUE REQUIREMENT DAILY MX ,WEEK COLIFORM, FECAL MF, M-FC SAMPLE <3 7 ( O 5/30 GRAB BROTH, 44.5C MEASUREMENT (13) 5 31616 1 0 0 PERMIT G******* RAB"3 EFFLUENT GROSS VALUE REQUIREMENT 200... 1000. DAIY ML ML WEEKLY ___________'M O AVG ' D'AILY M X i FLOW, IN CONDUIT OR THRU SAMPLE 0.046 0.124 (03) 0 30130 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT REPORT REPORT MGD CONTIN-RCORDR EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX s_ CHLORINE, TOTAL RESIDUAL SAMPLE Not (19) MEASUREMENT Chlorinating 50060 1 0 0 PERMIT

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MG/L 'r; !WEEK- ' !'GRAB, EFFLUENT GROSS VALUE REQUIREMENT DAILY M ' DAS t.*, SAMPLE MEASUREMENT PERMIT REQUIREMENT. .' n .:i_ NAMETITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attadments were prepared under my TELEPHONE DATE direction or supervision In accordance vith a systern designed to assure that qualified personnel W. R. Lagergren properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system or those persons directly responsible for gathering the information, the Information submitted is, to the best of my knovwedge and belief. bue, acrate. 423 365-8767 03 05 15 SITE VICE PRESIDENT and complete. I am aware that there are significant penalties for submiting false Information. SGNA OF PR! IPAL EXECUTIVE TYPED PRl itiNiTED including the possibility of fine and imprisonmert for knowing violations. oAER OR AUTHiORiED AGENT ARA NUMBER YER MO DAY COMMENTS AND EXPLANATION OF ANY MOLATIONS (eference ol ttchments here) Prnvint,~ ~ditnn~ rn~tv h~ l5nd Paoel10ofl rn A IT 'I'Mo. 4 PW t S O Prpvinms pitinns Mn1V hp (tlsed Page of

PERMITTEE NAME/ADDRESS (Include Facilirv Name/Location if Different) ame TVA - WATTS BAR NUCLEAR PLANT Address.pA BQX _zlFFC Mg IEI?1fGEaM T -O7fl - - FAtln: J A wf EreL _u_e_v_ Attn: Robert J. Crawford. Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) SUBR01 TNO020168 112 G I F-FINAL PERMIT NMBER IDISCHRGE NUMBER RUNOFF HOLDING POND I-MONITOPINC, PFPIOD F Vr AQ M) lnAV To VI o I I From 03 04 01 To 03l0 30 1 Form Approved. OMB No. 2040-0004 I EFFLUENT

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-; ;&*-m SOLIDS, TOTAL SUSPENDED SAMPLE 11 16 (19) 0 5/30 GRAB MEASUREMENT 00530 1 0 0 PERMIT 30 100 MGIL WEEKLY. rGRAB-? EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX . S. 4x' NITROGEN, AMMONIA TOTAL (AS SAMPLE <0.06 0.15 (19) 0 5/30 GRAB N) MEASUREMENT 00610 1 0 0 PERMIT 1.46 2.42 MGtL WEEKLY ,GRAB, EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX

M 10

+ 'W8 FLOW, IN CONDUIT OR THRU SAMPLE 0.371 0.654 (03) 0 5/30 INSTAN TREATMENT PLANT MEASUREMENT 5050 1 0 0 PERMIT REPORT REPORT MGD C WEEKLY INSTAN~ EFFLUENT GROSS VALUE REQUIREMENT MOA G DAILY MX i CHLORINE, TOTAL RESIDUAL SAMPLE <0.02 <0.02 ( 0 5/30 GRAB MEASUREMENT (19) 50060 1 0 0 PERMIT .011 -'019 MGIL WEEKLYi -;"GRAB't EFFLUENT GROSS VALUE REQUIREMENT IMO AVG DALY MX i_____ SAMPLE MEASUREMENT PERMIT REQUIREMENT ~~Z NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that ts documert and all aachments were prepared under my TELEPHONE DATE direction or supervision In acordance vith a system designed to assure that qualified personnel W. R. Lagergren properly gather and evaluate the iformation submitted. Based on my inquiry of the peron or . R. Lagergren persons who manage the system, or those persons directly responsible for gathering the information, th nformation submitted Is, to the best of my knowledge and belief. tue. acurate, 423 365-8767 03 05 15 SITE VICE PRESIDENT and complete. I am aware that there are significant penalies for submitting false nformation, SIGN&URE OF PRINePAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and Imprsonment for knoving violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (eference oJ1 ottochments here) -4 t bes n_..;^-, hWie .hefe Page 1 of

PERMITTEE NAMEIADDRESS (Include FacilityNameltocation ifDifferent) NOmRe TVA - WATTS BAR NUCLEAR PLANT dr.Lsp,

iEQ X_5 -

_ _ _ __PWFfI5R_ E6 E - Fopt :!V/hEARgI L.ElJL A t n o r J.A C r Tad E n vi o n m _e-v-o Attn: Robert J. Crawford, Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) SUBR 01 I TN0020168 PERMT NUMBER Form Approved. OMB No. 2040-0004 1 112 T I F-FINAL _ JDISCHARGE NUMR IOMONITORING FOR OUTFALL 112 KAnKNITnDITDlNlf2 DCIt FrFo I l IAYm YAQ l I nAV From 03 04 01 To 0N3 1 04 309 EFFLUENT

- NO DISCHARGE NOTE: Read Instructions before comletino fis form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE ~~~. EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALY IC25 STATRE 7DAY CHR SAMPLE >100 (23) 0 1/180 COMPOS CERIODAPHNIA MEASUREMENT RP38 1 0 0 PERMIT .100 PERCENT :; 'SEMI-, COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM 'ANNUAL :a;'V. IC25 STATRE 7DAY CHR SAMPLE 82

23) 1 1/180 COMPOS PIMEPHALES MEASUREMENT 82 (2)

P6C 1 0 0 PERMIT 100 PERCENT ;,- SEMI-COMPOS EFFLUENT GROSS VALUE RUIEETMINIMUM

ANNUAL, SAMPLE MEASUREMENT PERmITrr REQUIREMENT SAMPLE MEASUREMENT PERMIT

~.4~ REQUIREMENT ~, ~ ~ "' -~ SAMPLE MEASUREMENT PERMIT I REOUIREMENT k4 SAMPLE J MEASUREMENT PERMIT REQUIREMENT i, SAMPLE MEASUREMENT PERMITr REQUIREMENT j'..;._ NAMETTLE PR1NCIPAL EXECUTIVE OFFICER I Certify under penalty of law that tNs document and all attachments were prepared under my TELEPHONE DATE direction or superislon in accordance with a system designed to assure that qualified personnel / W. R. Lagergren properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the intoration, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 365-8767 03 05 15 SITE VICE PRESIDENT and complete. I am aware that there are significant penalties for submitting false Informtion, SIGNA OF PRINCIPA EXECUTVE inctuting t possibiity of fine and Imprisonment for knong violations. OfFI ROR AUTHOIZEDAGENT AJlNBER YEAQ MO DAY TYPED OR PRINTED ID OFII ORAT_1E GN NME ER M A COMMENTS AND EXPLANATION OF ANY VlOLATIONS (Reference al attochments here) See attached Notice of Noncompliance. Repeat toxicity testing on Pimephales was performed April 27-May 2, 2003. The report has not been reviewed. The retest test report will be sent with the next DMR along with a correction for this DMR. I -. 11 r-s f -n r)nt-'-- riio-nl h llsPd Page 1 of 1

PERMlTEE NAME/ADDRESS (Include Faclit NameAocation if Different)

Nnm, IVA - WATTS BAR NUCLEAR PLANT

.AddLa.ss _P,Q QUQQQ__ _ F Q i tV J a z S A T Q I Z E A tIN L__ sgnEnE 5 )-- Attn: Robert J. Crawford. Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES) MAJOR DISCHARGE MONITORING REPORT (OMRJ 5 TNO020168 I PERMIT NUMBER SUBR 01 1 113 G I F-FINAL IDISCHADRGE i UMBRP SCCW DISCHARGE FrAo m MO nAV lI To I From 03 l 04 01 To l0 Form Approved. OMB No. 2040-0004 EFFLUENT .MO I AY NO DISCHARGE [a NOTE.: Read instructions before comoletirta this form. PARAMETER QUANTITY OR LOADING CtUA11Y OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNrrS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 18.5 (04) 0 30/30 RCORDR CENTIGRADE MEASUREMENT 00010 P 0 0 PERMIT 33.5 DEG. C. HOURLY RCORDR Temp, Receiving Stream Btm REQUIREMENT DAILY MX t __.i___ TEMPERATURE, WATER DEG. SAMPLE 18.1 (04) 0 30/30 RCORDR CENTIGRADE MEASUREMENT 00010 Z 0 0 PERMIT 30.5 DEG. C. HOURLY, RCORDR Instream Edge of Mixing Zone REQUIREMENT DAILY MX "I__, z: t TEMPERATURE, WATER DEG. SAMPLE 30 (04) 0 30/30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 0 PERMIT REPORT DEG. C. CONTIN-RCORDR EFFLUENT GROSS VALUE REQUIREMENT

-MX o

tiU. $ TEMP. DIFF. BETWEEN SAMP. & SAMPLE (04) 1 04 0 30/30 CALCTD UPSTRM DEG.C MEASUREMENT (0) 00016 Z 0 0 PERMITr DEG. C.

,3 DEG. C.

HOURLY, CALCTD,, Temp, Rise UpStrm to DnStrm RERM DAILY MX D C __HORLY_ OXYGEN, DISSOLVED (DO) SAMPLE 8.8 (19) 0 1 /30 GRAB MEASUREMENT 00300 1 0 0 PERMIT REPORT MGIL ;7 OGRAB EFFLUENT GROSS VALUE REQUIREMENT ._:_._DAILY MN 4.MONTH' PH ~~~~~~~~MEASUREMENT 8.3 8.3 (12) 0 1/0 GA oo400 1 0 0 PERMiT 6.0

9.0 Su ONCE/

GRAB:- EFFLUENT GROSS VALUE REQUIREMENT MINIMUM MAXIMUM MONTH SOLIDS, TOTAL SUSPENDED SAMPLE 5 5 (19) 0 1 /30 GRAB MEASUREMENT .19 00530 1 0 0 PERMIT REPORT . REPORT MGIL. ONCE/,. GRABjS EFFLUENT GROSS VALUE REQUIREMENT MO AVG I DAILY MX i :MONTH,' NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certfy uncde penalty of aw tat this document and att attahmnts were prepared under my TELEPHONE DATE irection or supervision in accordance with a system designed to assue that qualified personnel W. R. Lagergren spopy gatr and evaluatb the Wormation submitted Based on m ry Inqluiry oJf this person or ///7 /A persons who manage the system, or those persons dirctly responsible for gathenng the information, the information submitted is, to the best of my knowledge andi biet true aurate-423 365-8767 03 05 15 SITE VICE PRESIDENT and cplete. I am ware that there are significant penalties for submitting false information, SIGNATURffFt EXETVE including the possibility of fine and imprisonment for knoving violations OFFiR OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR P9tNTED.-.. tn COMMENTS AND EXPLANATION OF ANY \\tOLATIONS (Reference ol attachments here) Instream flowof> 3500 cfs present as required bythe permit. 0000Z =Instream temp. atedge ofthe Mixing Zone. 00010P = Instream temp. at Receiving Stream bottom. Stream Flowdirection indicates maximum daily percentage of time flow was upstream. -. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~4 ~~~~~~ ~ ~ ~ ~ 0 l-. ¶r. r~ 30on NOTE:,4 Read~n Insntclon befor cornd Pae JI v - l L Uevl I MOAnKlTtnDlNAe =DiI on 4 ^-

-n 4 197CA? IMet Ppviiot AdiifinnT m;?v e sed Page of 2

PERMITTEENAME/ADDRESS (Inctude Faciit Nametocation ifDfferent) Nrme_ VA - WATrS BAR NUCLEAR PLANT £ 1--tY- -TVM --WAEA ~U`L Affn: Rober J. Crawford, Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM NPDES) MAJOR DISCHARGE MONITORING REPORT OMR) SUBR 01 TN0020168 ll 113 G F - FINAL I .-PERITNM I DSGHARGE NMBER SCCW DISCHARGE I nNITQPINr-PFPIQC) I EFFLUENT IYFARI? MC r I I lrVARl

I*

mR flfltS*A Froml 03 04 01 Tol 03 04 30 Form Approved. OMB No. 2040-0004 NOTE: Read instructions before completina this form. PARAMETER QUANTiY OR LOADING QUALITY OR CONCENTRATiON NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALSS FLOW, IN CONDUIT OR THRU SAMPLE 168.148 188.499 (03) 30 / 30 RCORDR TREATMENT PLANT MEASUREMENT (3 50050 1 0 0 PERMIT REPORT REPORT: MGD CONTIN-RCORDR EFFLUENT GROSS VALUE REQUIREMENT MO AVG_ DAILY MX SoUS 70 } CHLORINE, TOTAL RESIDUAL SAMPLE <0.020 <0.020 (19) 0 1/30 GRAB MEASUREMENT 50060 1 0 0 PERMIT 0.092 0.158 MG/L ONCE/,

GRAB1 EFFLUENT GROSS VALUE REQUIREMENT MOAVG DAILYMX MONTH TEMPERATURE - C, RATE OF SAMPLE 1

(04) 0 30/30 CALCTD CHANGE MEASUREMENT 82234 Z 0 0 PERMIT 2 DEG. C. HOURLYI CALCTDi Temp, Rate of Chng DnStrm REQUIREMENT DAILYMX DISCHARGE EVENT OBSERVATION SAMPLE YES (94 0 1/30 OPRCRD MEASUREMENT (94) 84165 1 0 0 PERMIT REPORT Y=1;N=0 MONTHLY OPRCRD 'EFFLUENT GROSS VALUE REQUIREMENT CERT YES/NO STREAM FLOW DIRECTION SAMPLE 33 0 30 /30 RCORDR RECORDING MEASUREMENT 50052 1 0 0 PERMIT Flo Upstrm % TIME g3DAILYJr RCORDR REQUIREMENT ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~rW~% ~ EFFLUENT GROSS VALUE REQUIREMENTDAILY, DA MX f SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT REQUI NT~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAME/nTLE PRINCIPAL EXECUTIVE OFFICER I Cerfy under penally of law that this document and all atachmenls were prepared under my TELEPHONE DATE direction or supervision in accordance with a syslem designed to assure that qualified personnel W. R. Lagergren propery gather and evaluate the information submited. Based on my Inquiry of the person or persons womanage the system, or ose persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, actce 423 365-8767 03 05 15 SITE VICE PRESIDENT and complete. I am aware thal there are significant penalties for submitting false Information, SIGNA PALEXECUTIVE irr_uding the possibility of line and kiprisonment for knowing violations. OFFICER OR AUTFORiZED AGENT AREA YEAR MO DAY TYPED OR PrNNTED COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference oll attachments here) Instream flow of > 3500 cfs present as required by the permit. OOOIOZ = Instream temp. at edge of the Mixing Zone. 0001OP = Instream temp. at Receiving Stream bottom. Stream Flow direction Indicates maximum daily percentage of time flow was upstream. Page 2 of 2 . r--- i 1 5-.-i I.-- S -. 4en

PERMrTTEE NAMEIADDRESS (Include FaciEtv Name/Location if Different) NmAXm TVA - WATTS BAR NUCLEAR PLANT - - - iT WjL9nXM 17a] - f9tYn?tEA zCWNTY _ _ _ _A_A_ _-_ _ _-_ Attn: Robert J. Crawford. Environmental Supervisor NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGEMONITORING REPORT (OMR) SUBR 01 TN0020168 113 T I F - FINAL I PERMIT NUMBER-MQrNIaQn VFAQP M I rDAV l Froml 03 04 01 I Form Approved. OMB No. 2040-0004 I DISCHARGE NUMBERI BIOMONITORING FOR OUTFALL 113 f1N(:: PFF0nr -- EFFLUENT To V _= I AV NO DISCHARGE [ NOTE: Read nstructions before completing this form. I PARAMETER QUANTITY OR LOADING QUALTY OR CONCENTPATION NO. FREQUENCY SAMPLE EX~~~~~~~~~~~~~~~~~~~~~~~~~ OF TYPE AVERAGE MAXIMUM UNTS MINIMUM AVERAGE MAXMUM UNITS ANALYStS IC25 STATRE 7DAY CHR SAMPLE >4 1.2 (23) 0 1/180 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 PERMIT 10.3 PERCENT,- s SEMI-r COMPOS EFFLUENT GROSS VALUE RUIENTMINIMUM ~ -ANNUAU. IC25 STATRE 7DAY CHR SAMPLE >41.2 (23) 0 1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C I 0 0 PERMIT 10.3 PERCENT ir u.SEMIl.'d COMPOS EFFLUENT GROSS VALUE REURMN MNMM ~ANNUfAV" SAMPLE MEASUREMENT PERMIT k" !r' REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT .0-. 1 REQUIREMENT SAMPLE MEASUREMENT PERMIT .p-. r... REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT ertity under penalty of lw that this document and all attachments were prepared under my ction or supervision in accordance with a system designed to assure that qualified personnel perty gather and evaluate the Information submitted. Based on my Inquiry of the person or sons who manage the system, or those persons directly responsible for gathering the rmtion the information submitted is, to the best of my knowledge and belief, true, scrate. I comp,lete. I am aware that there are significant penalties for submitting false information. uding the possibility of fine and mprisonment for knowing violations. Page 1 of 1 T COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference oll ottachments here) Instream flowof >3500 cfs present as required bythe permit. 00010Z= Instream temp. at edge of the Mixing Zone. 0010P =Instream temp. at Receiving Stream bottom. Stream flowdirection indicates maximum daily percentage of time flow was upstream. i 1.11. I llv-t . -4 I ' i, " '! 1-- `N "41 1 -1;, ^,,

TENNESSEE VALLEY AUTHORITY WATTS BAR NUCLEAR PLANT TOXICITY TESTING, OUTFALLS 101, 112, AND 113 EXECUTIVE

SUMMARY

Toxicity testing was conducted on Outfalls 101, 112, and 113 during April 2003, to meet the requirements of the Watts Bar Nuclear Plant (WBN) NPDES permit (TN0020168). Composite samples collected from Outfalls 101, 112, and 113 were analyzed for toxicity using definitive, short-term chronic toxicity tests with fathead minnows, Pimephales promelas, and daphnids, Ceriodaphnia dubia. Acute (96-hour LCso) and chronic (IC2 5) endpoints were calculated for each effluent. -Toxicity tests were conducted according to U.S. EPA (EPA/821/R/02/013) Method 1000.0 for Pimephales and Method 1002.0 for Ceriodaphnia. Toxicity test results follow: Outfall 101 Composite samples of Outfall 101 and grab samples of raw water intake were collected April 13-18, 2003. The toxicity tests for fathead minnows and daphnid were conducted April 14-21, using the specified test dilution series of 0.83, 1.7, 3.3, 6.6, and 13.2 percent concentrations of Outfall 101. The resulting IC25 values for fathead minnow and daphnids were > 13.2 percent, which meets the permit limit of 3.3 percent. Intake samples were tested at 100 percent only, for both tests. Fathead minnows exposed to intake samples were significantly different from control for survival and growth based on Homoscedastic t-Test. There was no significant difference between control and intake samples for daphnid survival or reproduction based on Homoscedastic t-Test. Outfall 112 Composite samples of Outfall -1 12 were collected April 13-18, 2003. Toxicity tests were conducted April 15-22, using the specified dilution series of 6.25, 12.5, 25.0, 50.0, and 100 percent concentrations of Outfall 112. The resulting IC25 value for daphnid test was > 100 percent, which meets the permit limit of 100 percent. Testing of fathead minnows, however, resulted in an IC25 of 82.5 percent effluent (1.2 TUc) due to significant and highly variable mortality observed throughout the Outfall 112 serial dilution series. This pattern of mortality has been observed previously in WBN Outfall 112 samples and it may to be related to ambient conditions upstream of the construction runoff holding pond, possibly due to the presence of pathogens or other biological organisms. Bacterial growth was observed on the gills of minnows exposed to non-treated effluent from Outfall 112. This pathogenic growth was likely the cause of mortality in the effluent.

4-A repeat test with fathead minnows was conducted April 29-May 6, due to test results in April which exceeded the permit limit for Outfall 112. Surface stream samples collected upstream of the sewage treatment pond (NOSTP) discharge (Outfall 111) was also tested as part of this study to evaluate the influence of individual inflows on the quality of Outfall 112. Fathead minnows were also exposed to UV treated Outfall 112 samples since fish pathogens present in upstream water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Watts Bar Nuclear Plant. Survival was 100% in all effluent concentrations with two-minute exposure to UV light prior to introduction of test organisms. These results suggest that fish pathogen interference is a likely cause of minnow mortality in the effluent. The resulting IC25 value for UV-treated effluent was > 100 percent for fathead minnows. Outfall 113 Composite samples of Outfall 113 and grab samples of raw water intake were collected April 13-18, 2003. Toxicity tests were conducted April 15-22, using the specified dilution series of 2.58, 5.20, 10.3, 20.6, and 41.2 percent concentrations of Outfall 113. The resulting IC25 values for fathead minnows and daphnids were > 41.2 percent, which meets the permit limit of 10.3 percent. Intake samples were tested at 100 percent only, for both tests. Analyses using the Homoscedastic t-Test showed no toxicity to fathead minnows or daphnids exposed to intake samples. 2

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE

SUMMARY

1)

Facility/Discharger: Watts Bar Nuclear Plant/TVA Report Date: May 13, 2003

2)

County/State: Rhea/Tennessee

3) NPDES Permit#:

TN0020168

4)

Type of Facility: Nuclear-fueled electric generating plant

5)

Average Flow (MGD): 66.4

6)

Receiving Stream: TRM 527.9

7) 1Q20 (MGD): 2062

8)

Outfall(s) Tested: 101

9) Dates Sampled: April 13-18. 2003

10) Flow on day(s) sampled (MGD): 22.25 20.22 19.05 19.20 18.92 10.03

11) Pertinent site conditions:

Watts Bar Hydro and WBN DSN101 operations during this study are summarized in Appendix B. Dilution factors ranged from 491 to 1089. There were no periods of zero release. During the toxicity testing; the daily periodic bromine/chlorine treatment of WBN cooling water was in service.

12) Test Dates: April 14-21. 2003

13) Test Type: Short-term Chronic-definitive

14) Test Species: #1 Fathead Minnows (Pimephales pronzelas)

2 Daphnids (Ceriodaphnia dutbia)

15) Concentrations Tested (%): Outfall 101 0.83 1.7 3.3 6.6 13.2 Raw Water Intake 100

16) Permit Limit Endpoint (%): I 5 = 3.3

17) Test Results (%):Pimephalespronelas: IC75_> 13.2 Ceriodaphiia dutbia: IC25 > 13.2

18) Facility

Contact:

Martha I. Ervin

19) Phone : (423) 365-3585

20) Testing Lab Name: S&ME, Inc.

21) Lab

Contact:

Leira S. Douthat

22) Phone #: (865) 970-0003 TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

i

23) Notes: Exposure of test organisms to samples from Outfall 101 resulted in no toxicity to Pimephalespromelas or Ceriodaphnia dubia. Fathead minnows exposed to intake samples were significantly different from control for survival and growth based on Homoscedastic t-Test. There was no significant difference between control and intake samples for daphnid survival or reproduction based on Homoscedastic t-Test.

METHODS

SUMMARY

Samples

1) SamplingPoint:

Outfall 101 -DiffuserDischarge:Intake

2) Sample Type:

Outfall 101 -Composite; Intake-Grab

3) Sample Information:

Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp. TRC* Used By (MM-DD/Time) (MM-DD/Time) (°C) (mg/L) (MM-DD/Time) 04-13/0805 to 04-14/ 1400 101 04-14/0705 04-14/1250 1.0 0.05 04-15/1445 Intake 04-14/0845 04-14/1250 1.5 0.04 04-14/1400 04-15/0850 to 04-16/1400 101 04-16/0750 04-16/1158 1.2 0.07 04-17/1420 Intake 04-16/0735 04-16/1158 0.9 0.07 04-16/1400 04-18/1445 04-17/0845 to 04-18/1445 101 04-18/0745 04-18/1228 1.9 0.05 04-19/1310 04-20/1305 04-18/1445 Intake 04-18/0830 04-18/1228 2.0 0.06 04-19/1310 04-20/1305

Total residual chlorine. Interference likely since chlorine would not be expected in ambient (intake) water.

NOTE: All times listed are Eastern Daylight Savings Time.

4) Sample manipulation: Samples were warmed to test temperature (25°C) in a warm water bath Test Organisms

1) Source:

2) Age:

Pimephales promelas In-house culture < 24 hours Ceriodaphnia dubia In-house culture < 24 hours 2 j.

Test Method Summarv (See Appendix A for additional information) Test Conditions Test Duration

3) Dilution/Control Water Pimephales promelas Static, renewal 7 days Moderately Hard Reconstituted Water Ceriodaphnia dubia Static, renewal Until 60% of control females have 3 broods 20% Dilute Mineral Water with trace minerals

4) Number Replicates

5) Animals per Replicate

6) Test Initiation (101)

7) Test Termination (101)

8) Test Temperature (101) 4-14-03/1330 EDT 4-21-03/1400 EDT Mean = 25.1 °C (24.0°C-25.90C) 4-14-03/1400 EDT 4-21-03/1350 EDT Mean = 25.1°C (24.0°C-25.80C)

9) PhysicalChemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample or the highest concentration tested. Daily temperatures were measured in one replicate in each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical softvare (Tidepool Scientific Software, McKinneyville, CA).

3 1) 2) 4 10 10 1

1)

Results of a Pinephales promelas (Genus) (Species) Conducted 04/14/03 - 04/21/03 (mm/dd/yy) (mm/dd/yy) Larval Survival and Growth/7-days Toxicity Test (Type/Duration) Using Effluent From: Outfall 101 (number) Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4* 5 6 7 Control 100 100 98 98 98 98 98 0.83% Effluent 100 100 100 100 100 98 98 1.7% Effluent 100 100 98 98 98 98 98 3.3% Effluent 100 100 98 98 98 98 95 6.6% Effluent 100 100 100 98 95 95 93 13.2% Effluent 100 100 100 100 98 93 90 Intake 100 98 90 88 80 58 53 Calculated TU Estimate: <7.58 TUa

96-Hour LC5o Value: > 13.2%

Permit Limit: N/A IC25 Mean Dry Weight (mg) Test Solutions (Replicate) l 1 2 3 4 l Mean Control 0.439 0.512 0.470 0.603 0.506 0.83% Effluent 0.298 0.480 0.467 0.521 0.442 1.7% Effluent 0.459 0.453 0.501 0.510 0.481 3.3% Effluent 0.415 0.319 0.455 0.375 0.391 6.6% Effluent 0.371 0.447 0.425 0.544 0.447 13.2% Effluent 0.359 0.448 0.501 0.378 0.422 Intake 0.155 0.070 0.286 0.516 0.257 IC25 Value: > 13.2% Calculated TU Estimatest <7.58 TUc Permit Limit= 3.3% Permit Limit: 30.30 TUc TNOTE: TUJa = 100/LC5 0; TUc =100/ IC25 4 a

2)

Results of a Ceriodaphnia dubia (Genus) (Species) Conducted 4/14/03 - 4/21/03 (mm/dd/yy) (mm/ddlyy) Chronic/ 7-day (3-brood) Toxicity Test (Type/Duration) Using Effluent From: Outfall 101. (number) Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4* 5 6 7 Control 100 100 100 90 90 90 90 0.83% Effluent 100 100 100 100 90 90 90 1.7% Effluent 100 100 100 100 100 100 100 3.3% Effluent 100 100 100 100 100 100 100 6.6% Effluent 100 100 90 90 90 90 90 13.2% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Calculated TU Estimate: <7.58 TUa

96-Hour LC5 0 Value: > 13.2%

Permit Limit: N/A Reproduction (#young/female/6 days) Data Test Solutions Replicates 1 2 3 4 5 6 7 8 9 10 Mean Control 31 31 25 23 29 29 0 27 27 29 25.1 0.83% Effluent 27 4 23 22 32 26 28 27 28 21 23.8 1.7% Effluent 27 31 32 31 24 24 27 24 20 24 26.4 3.3% Effluent 23 33 27 28 24 23 27 25 30 23 26.3 6.6% Effluent 0 32 24 26 27 21 22 26 24 23 22.5 .13.2% Effluent 29 30 22 29 23 22 23 21 28 25 25.2 Intake 35 25 23 25 25 31 22 28 31 35 28.0 IC25 Value: > 13.2% Calculated TU Estimatest <7.58 TUc Permit Limit= 3.3% Permit Limit: 30.30 TUc tNOTE: TUa = 1 00/LC5o; TUc= 100/IC25 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D) Species I Date I Time I Duration I Toxicant I Results (LC50/IC25) Pirnephales promelas 04/08/03 1300 7 days KCI 801.69 mg/L (IC25) Ceriodaphnia dutbia 04/08/03 1345 7 days NaCI 1106.64 mg/L (IC25) ll 5 X

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Fathead Minnow Test, Watts Bar Nuclear Plant, WBN, Outfall 101, April 14-21, 2003 6 Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC) (OC)

(mg/L)

(mg/L) S.U. S.U. (jimhos) mg/L CaCO3 mg/L CaCO3 (mg/L) Fathead/ 25.2 25.1 6.7 5.8 8.0 7.6 390 62.5 94.3 Control (24.2-25.8) (24.8-25.6) (6.2-7.2) (5.2-6.3) (7.9-8.1) (7.3-7.9) (377-401) (60-64) (80-100) Fathead/ 25.3 24.9 6.7 5.8 8.0 7.6 387 0.83% (24.3-25.8) (24.5-25.3) (6.2-7.1) (5.3-6.5) (7.9-8.1) (7.2-7.9) (375-399) Fathead/ 25.3 25.0 6.6 5.6 8.0 7.6 385 1.7% (24.3-25.8) (24.6-25.2) (6.3-7.0) (4.9-6.5) (7.9-8:2) (7;3-7.8) (373-397) Fathead/ 25.4 25.1 6.6 5.5 8.1 7.6 381 3.3% (24.3-25.8) (24.7-25.9) (6.2-7.1) (4.9-6.2) (7.9-8.2) (7.3-7.7) (369-393) Fathead/ 25.4 24.9 6.6 5.6 8.1 7.6 375 6.6% (24.3-25.9) (24.3-25.6) (6.3-7.0) (4.9-6.4) (8.0-8.2) (7.3-7.8) (363-387) Fathead/ 25.3 24.8 6.7 5.5 8.1 7.6 366 13.2% (24.3-25.8) (24.1-25.6) (6.3-7.1) (4.9-6.5) (8.0-8.2) (7.3-7.7) (350-374) Fathead/ 25.1 7.4 8.0 183 61.7 78 0.06 100% (24.2-25.9) (6.7-7.9) (7.9-8.1) (164-203) (60-65) (70-84) (0.05-0.07) Fathead/ 25.1 25.0 8.1 5.9 7.9 7.7 159 57 73.3 0.06 Intake (24.0-25.7) (24.6-25.3) (7.4-8.9) (5.3-6.7) (7.8-8.1) (7.5-8.0) (154-165) (54-59) (68-76) (0.04-0.07)

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for Daphnid Test, Watts Bar Nuclear Plant, WBN, Outfall 101, April 14-21, 2003 7 Temperature Dissolved Oxygen Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC) (OC) (mg/L) (mg/L) S.U. S.U. (jimhos) mg/L CaCO3 mg/L CaCO3 (mg/L) Daphnid/ 25.3 24.9 6.7 6.2 8.0 7.9 168 59.7 80.7 Control (24.2-25.8) (24.0-25.8) (6.5-7.0) (5.5-6.6) (8.0-8.1) (7.6-8.3) (163-175) (58-62) (80-82) Daphnid/ 25.3 24.9 6.7 6.2 8.1 8.0 167 0.83% (24.2-25.8) (24.1-25.6) (6.3-6.9) (5.5-6.5) (8.0-8.1) (7.7-8.2) (164-169) Daphnid/ 25.3 24.9 6.7 6.2 8.1 8.1 167 1.7% (24.3-25.8) (24.1-25.5) (6.2-7.0) (5.5-6.5) (8.0-8.2) (7.7-8.3) (165-169) Daphnid/ 25.3 24.9 6.7 6.2 8.1 8.1 168 3.3% (24.3-25.7) (24.2-25.5) (6.2-7.0) (5.5-6.5) (8.0-8.1) (7.7-8.2) (165-170) Daphnid/ 25.3 24.9 6.7 6.2 8.1 8.1 169 6.6% (24.5-25.6) (24.2-25.5) (6.3-7.0) (5.5-6.6) (8.0-8.1) (7.7-8.2)

(166-171)

Daphnid/ 25.2 25.0 6.7 6.2 8.1 8.1 170 13.2% (24.5-25.6) (24.3-25.5) (6.3-7.1) (5.5-6.8) (8.0-8.1) (7.7-8.2) (167-174) Daphnid/ 25.2 7.5 8.0 180 61.7 78 0.06 100% (24.6-25.8) (6.8-8.1) (7.9-8.1) (161-203) (60-65) (70-84) (0.05-0.07) Daphnid/ 25.1 24.8 8.1 6.3 8.0 8.2 159 57 73.3 0.06 Intake (24.1-25.7) (24.2-25.2) (7.2-8.8) (5.8-6.6) (7.8-8.5) (7.7-8.3) (149-165) (54-59) (68-76) (0.04-0.07)

SUMMARY

/CONCLUSIONS Exposure of test organisms to samples from Outfall 101 resulted in no toxicity to Pimephales promelas or Ceriodaphnia dubia. Fathead minnows exposed to intake samples were significantly different from control for survive and growth based on Homoscedastic t-Test. There was no significant difference between control and intake samples for daphnid survival or reproduction based on Homoscedastic t-Test. 8

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS Fathead minnow tests were conducted according to EPA/821/R/02/013 using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL polystyrene tumblers, each containing 250 mL of test solution. [2] C. dubia tests were conducted according to EPA1821/R/02/013 using ten replicates, each containing one test organism. Test vessels consisted of 30-mL polystyrene containers, each containing 15 mL of test solution. DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL

1) Pimephales promelas None

2) Ceriodaphnia dubia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1) Pimephales promelas None

2) Ceriodaphnia dutbia None

-9

PHYSICAL AND CHEMICAL METHODS

1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2) Instruments: All identification, service, and calibration information pertaining to S&ME laboratory instruments is recorded in calibration and maintenance log books.

3) Temperature was measured using a VWR Scientific digital NIST-traceable thermometer according to S&ME SOP. [3]

4) Dissolved oxygen was measured using a VWR Scientific Dissolved oxygen meter.

The instrument was calibrated and readings were made according to EPA Method 120.1.

5) The pH was measured using a VWR Scientific Symphony pH meter equipped with an Orion combination electrode. The instrument was calibrated and readings were made according to EPA Method 150.1.

6) Conductance was measured using an Orion conductivity meter. The instrument was calibrated and readings were taken according to manufacturers instructions.

7) Alkalinity was measured using Standard Methods Titration Method 2320 B using 0.01 N HCl to an endpoint pH of 4.5.

8) Hardness was measured using Standard Methods EDTA Titrimetric Method 2340 C or EPA Method 130.2.

9) Total residual chlorine was determined using the DPD Ferrous Titrimetric Method with a Hach Colorimetric kit.

10

OUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage; glassware preparation; test organism culturing/acquisition and acclimation; test organism handling during test; and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/821/R102/013. [2] Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1) Test Type: 7-day chronic tests with results expressed as IC2 5 values in mg NaCl/L for Ceriodaphnia dubia or KCL/L for Pimep hales promelas.

2) Standard Toxicant: Sodium Chloride (NaCl crystalline) and Potassium Chloride (KCI crystalline)

3) Dilution Water Used: 20% Dilute Mineral Water with trace metals for Ceriodaphnia dutbia and Moderately Hard Water for Pimephales promelas.

4) Statistics: IC25 - calculated by ToxCalc version 5.0 statistical software using EPA-recommended methods.

REFERENCES

1) NPDES Permit No. TN0020168.

2) U.S. Environmental Protection Agency, Office of Water. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/821/R/02/013 (October 2002).

3) S&ME Standard Operating Procedures. May 2002.

11

Watts Bar Nuclear Plant Biomonitoring April 14 - 21, 2003 Appendix B Pertinent Site Data

Calculated Dilution Based Watts Bar Nuclear Plant (WBN) and Watts Bar (WB) Hydro Plant Releases, April 14-21, 2003. Date Zero Release WB Hydro Hourly WBN DSNIOI Dilution TRO (mg/L) (Hours) Average Flow Daily Flow (WB Measured at the j (MGD) (MGD) Hydro/WBN) time of samples collection 13-Apr 0 10923 22.25 491 <0.02 14-Apr 0 10923 20.22 540 15-Apr 0 10923 19.05 574 16-Apr 0 10923 19.20 569 0.02 17-Apr 0 10923 18.92 577 0.02 18-Apr 0 10923 10.03 1089

Watts Bar Nuclear Plant Biomonitoring April 14 - 21, 2003 Appendix C

Chain of Custody Records Toxicity Test Bench Sheets Statistical Analyses

Clitmi: TVA Project Name: WIINP IIOTOXJCfl'Y P.O. Nuiba: WA Facility Sampld: Wat Bar Nudear Plant NPDES Nurnb: TN04168 ColleciedBy; DeI C 1tk BIOMONITORING. CIAiN OF CUSTGDY RECORD age or ij. I. i S &ME, Inc. 1413 'opsid Road Louisville, TN 37777 Phone: 865-970-0003 Fax: 865-970-0004 Delivered By (Cirle Ftu,x UPS e One): Du (C*e otr"(pciy:_____ Ceral Ctniniex: Contcl WBNP personnel at (423) 365-3575 i n PrvbemS ar. enreunrLrd.,PFaer nmber 1SOO323-4853 then ntmbcr to be dialE_ 5An.p¶e afrjva-pv'ko Cs - L, r Fitld Idnulifirca6in GnlI Ship. Collco on Dzrl'irnte Number & Flow R jEvcTrI? Simple De ciiton Comp. Temp. Val,uc (MOD) (Mxk as A ppdle. ) - t) CoItccacd -lT-Yci If Yt. No xTC T?131/4 AnTivalTup. BY tre _VN afn>; take I 0 1 - t CTn b Kz = WBN OSNI11-1Crnp Mn.i /-/i 0 0 [11-.2.5 3 1S .~~~~~~z $ .1 ~~~~~~~- S ~~ SO d-r- -u w= l Sampic CU5Sdy -Fill ]nPn To pflowuv I i .~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ fl rIi nite .U in P1 Vo z'/ / / Y/'v - C / g1 Insaicns; CEcynts should fill mll zreasfevcp( thosch che Thzk Llu" b1 t. B iomunilring tnple rrcpwrv bysiorn slvr at "c nit sh optnhern hilee Theboldr f trenjzaT4e it 36 houis rom 1he. irf: of cUezxion. ibucfor, pICe: aUUecl M tEp in nch a way thai ihe bb%oitcry vAil reMT the sar¶1es Yih amPle fmitiale 4SOtig Wi%hiu (at (ha e C=te. Sarple; shippcd orcrnighl n Ffiy via PedEx or UPS al . makcd fo Smdzy4elivey or thy wW ot ineuntil clx followig Mondy. r - - _1aaL I )I I LI °-

BIOMON[TORING CHAIN OF CUSTODY RECORD Page I of Clii(: TVA S & NIj I DelivaCed By (Circfe One): ProjectNamc: WBWPBIDTOXICIY 1413 Topside Road Fedx TnPS Bl I P.O.Nunber. N/A LAuisville, TN 37777 fer (spc_y S:ocility Snpled: Wat Blar tNuckYlelnt Ce4w Corrurenits Contact WflNPperscncil t (4231 65-3575 -acility Sampled: Wa fla4u~if ny mlerTa encont. s n 34 NPfES Nunbe TNU2O6CS Phone: 865-970-0003 then number to be dialod. CnlkeC4dDBy: 1-I%hnf &rikfl rax: 865-970-0004 CAIVr N,thols Fild IdzntilcaiJon (;rb Shp. Il D r NU,yUs FThw Ruiu.EYCI.1; Sanmpi DscHrp!ion Crmp. Vlu (,MOD) (M2jknsA-ppmp4ale) Ut'~r .... SL... ;.Dae b.rfle

9F;- rsrY

?e nT s

- N' tt

..) .9.r irys io r_ A t i Aly TI Appr- \\VJBN Intake I I-l. Grab~ HANMi-Za-7 ?(OnclID(ci...

JIfrTWi'fglT r

lt W7LZ -Cl 1c9 1- / 01 1c-1,== WBN Q&N '"l-2.Sg Spfc C dy -Pi In rom Tp Tdwn I~~~~~~~~~~~~ r Reltnquished By (Signvurc)- DateTime It1j/g I us8 A B 49-¶ Ae--' v Rccivcd By (Signahre): tl, o DurIIamc 'I-- -~~~~ /tj - '4CX aTw13 [T.tpilifirfl CIrnrs wh,?ld fill in 41 ntms except Ohis in he laboratory lls'hrEu& Wumaritning sanuplcs u preved by sWtfinj em 4Cand shppinz cm in it. he hol1m lfor t sh lc ki 36 hours frurte I dnt Ofcnltcdon. hcnr0M, p!easc olletl m sip in a wy twy I hi 1hurahcry a t ill i t he sampls wth arn;le beta to iitohle 'citing v0diAu tha11 nt freaie. Sltes ihiprd overaitg t an Fric.y qinFedae. IJPS musl x mTked for Smlay dlivny or thcy vill t anvt nnld he (Niownvrg Mcnday. .~~~~~- .R , YY- ___vms ~~~~~~~~~~z St A'l, I

BIOMNONIORING CHAIN OF CUSTODY RECORD Clieot:l TVA rjwat Na=e: WxYUP &t4oaxici+y ~~~~~~~~~~~~~~~~~~~~~t P.O. Number N/A Facility Sampled: tJ etft;5 4cr AJlupd ( } Ta T h +/- v i +/- u u u n ~ a l a ] T h s t 1~ S j t r, -- Ashe ilvf C - F DlWivered'By (Cicic(nc): 5urs Bus j;5CO&710 O0hcaFstVr) era Commnu Cotnia4 W&J1 per&rs I c > 4 ( y Q 2 3 ) § & * - $ 8

v * ; # t O n t ( p o gqs t

tn to U r f Jf 9 6 n o r r I tqOO-3 '5' 3 W'rn U rn, b +o bedaie4, .n Relinquish i 5 By (Stgna ): JV -rS 1-A 1E~ D)ateMlirg Received By (S1nature) I I I 1--- -I Oeit's5 P2Z0 -FvdEr rvctio I o Dudtcime ~~~~~~~~~~~~~~~~ r _\\ I I ..1-t 1 -/oF A...i., .-Pcd,r /t 3 /VWttjr9/Io Z-I PC 4,. I-bn'i:ons: Cliaus shuald fill n ailarr ep thos in Lhe 'lbo,21ury UsrYblotk. Hlornuiting "lanqi pracrvcd byitoring ihem u 4'C 2nd s 9 prilng ftru iu ice. Th bmi1d tmem fo ewh umple is 36 hous from the ticr of cnllecon. herro.t pl~se CvPcu nd ship I sti a Way thul d laborItry will rexlve the rnrples wA1 nIple rto thise iling within tha titme bnet Smples shiXvpntovemiti win Priuiy "&lkdFA xMllS must bernrhefnrSniicdaydeliwyouhfy Wilt Til tIieCinilthrfolnwing Mrn,a _~~~~~~~~~~~~~~~~~~~~~ age_ -t of-I, -801 kW

.~~~~~~~~ ~ ~~~~~~~~~~~~~~~~~~~~~~~~ jt. FATHEAD lUINNOW TST INFORMATION Test Name: TVA - Watts Bar Nuclear Plant (Outfall 101) Test Conducted From: 'lI tAI _(DayO)To lrH1cz _(Day 7) Sites/Concentrations:

1. <

4.

3, c_ ioo '-.

2.

0.3.5. (J.b 67

3.

1.-7 V.

6.

3t Stock (if applicable): Control Water Type (): Moderately Hard Water + Trace Minerals Other (descnbe): _ Dilution Water Type (1): Moderately Hard Water + Trace Minerals Other (descnbe): l Source of Test Larvae (): S&ME Lci 03 -C.2-3 3' Other (descnbe): ( i 13 /') Date Larvae Hatched: 4l1--4 hu o3 Hatch Time: lI 2C60-S Initials: ,) Record of Minor Test Non-Conformity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed y: i(Reviewer Initials) on 41.)1°3 (Date) REV.0I, JANUARYO0 (fathead nw t info)

Chang, T et

.~~~~~~~~~~~~~~~~~~~~~~~~~~o and Fathead Minnow Daily Test I.nformation Logsheet. Daily Test lITemperature Inforration Feeding Information Change, or Test information l (25vl° C) (feeding interval 6i lh) Termination Control Water Carboy Date and Incubator Temp. Therm Fed 100 uL Feeding Adto oment Initials CIC) No. Bn e Start Time End Time a.m. P.M. Shrimp Carbov # Date Prep. Day O as _5 Day I rh-w am: Cio JA I_ I_ _-PM: pf EI45 L l ~ o Day 2,'D Po ImanCto 40 oo LI I IL s Day ILA) 6 asc) s tbt LP= S30 lA 9g 5 qll Day 3 o P,W c Dt Day4 ppmO Day5,z s`505 Day6 S am: coo 4 3 qtDDab zD 'P m:1s3 1500 Day 7 aqI

Client: TVA Watts Bar - Ouffall 101 Location: Rhea County, Tennessee Analysts:... ,W.& Dates: - lLi-41 113 . q 1. 120-l 1 / CUMULATIVE SURVIVAL OF P. PROMELAS IN CHRONIC TEST': REPLICATE REPLICATE DAY CONC. Initials NO. A B C D .1 I O O 1 0 10 lrMo 2 I& 1 0 i D Control ,)p 3 I _ q I D Mod. p M 4 xo .\\ to Hard r 5 = 7) I 0 10 S}Jit 6 10 o 0 O 0 7 {o 1e 9 J/'0 1 1 IG 1b 1 -{n2 I C0 2 jlb 3 o o to tO p3 100 '0 0.83% f'o 1 4 1 'O LO Ouffall 101 oo 5 l lb V3 t a ytE 6 4 J0 .Ji O o NV____ 7 1 lo I 10 2 Ic 1 Io 0 1.7% 3 1 I 0% 9 ID Outfalll 1 4 t c o \\ .j. }O 5 Az Cl 1 c: 'ffMI% 6 C t IO _o_

9 7

i 1 9 lo 2 cI o( 3.3% lb 3 J IO IC Ouffall 101 f 4 x.r \\0 \\ Pn IJM 5 IM CS I 0 I I () Niiz 6 I 1Q 7 IOj I ) .,L2 ~O tJ1c 10\\(: 6.6% E2/so 3 to 10 O Outfall 101 2al% 4 to kO o jc 6 if) aO IO (__)_n 7 10 qS f oI CONC. Initials DAY. NO. A. B 2 1 t: 10 10 13.2% 3 to l o 2.. I Outfall 101 4 kc t MJ 6 Jo I 0 p0j%,- 7 t lO ,/ 1 I0 Q 0 ') t O 1o .2 ZR t s 8 O to Intake 3 to l Po9 24 9 -i i t£ A l fStA 6 4s e 10 I A1 uW 7 t IS , n \\ 2 3 .~ ~~ 6___ 4 5 ~~6 7 4 1 ___ = ___ = ___ _=___ 3 _6 7__ ___4 2 5 36 47 5 1 62 7 ___3 24 36 47__ t% -i f U-- n rA >411tgiv>- lwo-Lcn cf 6z,- Or ¢S 9-A I'n(Z o3 - - -4 c j.$ - k o .^

c 4 d t L

A 1 k. .b L d I ~~~~.% >1.i,, ;, s -.a t, -4 6 , v - I...1 D V I., n - - - - -,. "N - n I

FATHEAD MINNOW SEVEN DAY TEST RAW DATA SURVIVAL AND GROWTH Client: TVA Watts Bar - Ouffall 101 Test Dates:.M4 TestConductedBy( UA ) Comments:_ Date Weighed 4/9 l!3 4 %412/03 Began drying (dat Weights Conducted by: %k 4~ End of rrvinn (dt tltir 'Oven Temp 2} Jos (0c) I ; C ne) M)2i/6 tL~C) ne) 41 1 7 e37 'l LArn Groups Dry Pan Weight Exposure &

Live
Original Tare Dry Weight (Pan + Fish Mean Repl.

Rep. Fish Fish Weight (mg) Pan + Fish (mg) minus Tare) (mg) Dry Weight (mg) A I D I a296. S3 II I4 .D 1k Control B I ICS 1283.01 I__.__

5.

j5 C 1-0 128.2 J2. I \\ D I0 I Cl O la8. l I Qqs

Ik nt) u A

j 0( ),28.g 2 rqow t 0.83% B O to i3 . 1 I4 l) 0S.. Ouffall 101 C 10 10 i L 2 1 (.q(61 D o I ID j 0 P2 84 - I 9I.9 0; 'I \\ A I 0 J 126o.qq tvst 4 50 O\\.L 1.7% B C 290 I 62 O l ) S. Is

i. S O.;VS Ouffall 101 C

I 0 2Z91.e2. a .3 5.o O__G____ D I J10 l292.8?) IAq-c 7. 9 _5_. I 0 _c A j-i Cz I J G; l L () 41I 3.3% B C 19.e Q q Ouffall 101 C l9 S I j98

6 I5 130
Sl

_______~ o 1 I ) l 301 S 1 3O D

t.

5, E.1 D~~~~~~~~~~~~~~~~~~~ A j9 10 J2.9 i3c CI 6.6% B 9 J6 1qqI 2 is I Le r _4_. i -I Outfall 101 C t S) 1:2q6.?,0 1 IAq4. -4.asR o D tO 1 0 I23Pr. I 2 5..1 L O S44 A jfl IZh* f a96*.O 3 s9 13.2% B I 1 2-2 6 3 (a 1aO.' H .I Outfall 101 C I O093.i6 ,7 gs - ?.I I A_ D ~ J tC i. 3. &q 3I7 1 fD

f2 u O A

/. 4: c-L,Je. c Q _; t \\o r-s A-W eCL4.. -V p S Sc-;-. cA e-r - J.i\\fP r-j -, I r V-I te/tfime) ci 1r7~ It5 11 A ;

8 ~t -,}.4; FATHEAD MINNOW SEVEN DAY TEST: RAW DATA SURVIVAL AND GROWTH i........--

Oven Temp Client: TVA Watts Bar - Ouffall 101 Test Dates:

f41Y-1241C Test Conducted By: A .S O Comments: Date Weighed gj Began drying (date/time)__/2 6$ I 30 Weights Conducted y_ I End of drying (dateltime) gi2Z103 )3, I -

Live
Original Rep.

Fish Fish Pan Tare Weight (mg) Dry Weight Pan + Fish (mg) Groups Dry Weight (Pan + Fish Mean minus Tare) (mg) Dry Weight (mg) A 3 .l o ,.9s5 7.* S Intake B C) 10 Oc C> 100% C ( JO I2.32 iq l .8(o o_____ D ID O I OU fqg qLA 5_(O.I A A

=_._

C BD_ CA_ AC_ BD_ C_ _ __A_ A C_ __D_ A_ D IJ Exposure & Repi.

S. , I~:, '.,." Larval Fish Growth and Survival Test-7 Day Growth Start Dale: 4114103 13:30 i End Date: 4122103 14:00 Sarnple Date: Comments: Conc-%o Test ID: 101wbO4O3f Sample ID: TN0020168 Lab ID: S&ME Sample Type: EFF3-Power Plant Protocol: EPAF 91-EPA Freshwater Test Species: PP-Pimephales promelas 1 2 3 4 D-Control 0.4390 0.83 0.2980 1.7 0.4590 3.3 0.4150 6.6 0.3710 13.2 0.3590 0.5120 0.4700 0.4800 0.4670 0.4530 0.5010 0.3190 0.4550 0.4470 0.4250 0.4480 0.5010 0.6030 0.5210 0.5100 0.3750 0.5440 0.3780 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5060 1.0000 0.5060 0.4390 0.6030 14.081 4 0.5060 1.0000 0.83 0.4415 0.8725 0.4415 0.2980 0.5210 22.287 4 1.324 2.410 0.1174 0.4611 0.9113 1.7 0.4808 0.9501 0.4808 OA530 0.5100 6.015 4 0.518 2.410 0.1174 0.4611 0.9113 3.3 0.3910 0.7727 0.3910 0.3190 0.4550 14.848 4 2.361 2.410 0.1174-0.4198 0.8295 6.6 0.4468 0.8829 0.4468 0.3710 0.5440 16.177 4 1.217 2.410 0.1174 0.4198 0.8295 13.2 0.4215 0.8330 0.4215 0.3590 0.5010 15.510 4 1.735 2.410 0.1174 0.4198 0.8295 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97069 0.884 -0.2608 -0.1386 BarUetVs Test indicates equal variances (p = 0.63) 3.46342 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 13.2 >13.2 7.57576 0.11737 0.23196 0.00675 0.00474 0.26378 5,18 Linear Interpolation (80 Resamples) Point SD 95% CL(Exp) Skew IC05-ICi0 IC15 IC20 IC25 IC40 IC50 0.4679 1.9214 2.8998 >13.2 >13.2 >13.2 >13.2

indicates IC estimate less than the lowest concentration 1.07 0.9 -

0.8 - 0.7 - 0 0.6 - u)w °O 0.5-005. a 0 0.3 - L c-15 O 1 z -.D ~ 0.2-0.1 0.0 0 5 10 15 ToxCalc v5.0 Dose % Reviewed by: 10 p I. I F X I I fi F

i.

Page 1

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 4/14/03 13:30 Test ID: 101wbO4O3i Sample ID:.. TN0020168-NPDES Permit# End Date: 4/22103 14:00 Lab ID: S&ME SampleType:A EFF3-Power Plant Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: PP-Pimephales promelas C.mmpnts:-

>r:

Conc-% 1 .D-Control 04 100 0.1 2 390 0.5120 550 0.0700 . i.. 3 4 u.4UU 0.2860 0.6u3u 0.5160 Transform: Untransformed 1-Tailed isotonic Conc-%/. Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5060 1.0000 0.5060 0.4390 0.6030 14.081 4 0.5060 1.0000

100 0.2568 0.5074 02568 0.0700 0.5160 75.689 4

2.408 1.943 0.2011 0.2568 0.5074 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9637 0.749 0.79994 1.15323 F-Test indicates equal variances (p = 0.13) 7.43892 47.4683 Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates significant differences Linear Interpolation (80 Resamples) Point SD 95% CL(Exp) Skew IC05* 10.150 IC10* 20.301 IC 5' 30.451 IC20* 40.602 IC25* 50.752 IC40* 81.204 IC50 >100

indicates IC estimate less than the lowest concentration 1.0 0.9 0.8 0.7 0 0.6 In O0.5

'D 0.4 p,so = 3ci1 / 0.3 0.2 0.1 0.0 0 50 100 Dose % 150 Reviewed by: - I-- A7n i . a - -, I. I O'..-. ToxCalc v5.0 Page

.7 1~.-' Larval Fish Growth and Survival Test-7 Day Survival Start Date: 4114/03 13:30 Test ID: 101wbO4O3i Sample ID: TN0020168 -End Uate: 4/22/03 14:00 Lab ID: S&ME Sample Type: EFF3-Power Plant Sa'mple Date: Protocol: EPAF 91-EPA Freshwater Test Species: PP-Pimephales promelas Comments: Conc-% 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 100 0.3000 0.2000 0.6000 1.0000 Transform: Arcsin Square Root Conc-% Mean N-Mean Mean Min Max CV% N D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4

100 0.5250 0.5250 0.8353 0.4636 1.4120 50.726 4

1-Tailed t-Stat Critical MSD 2.722 2.353 OA986 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.83562 0.749 1.10492 2.85603 Equality of variance cannot be confirmed Hypothesis Test (1-tail, 0.05) Heteroscedastic t Test indicates significant differences Reviewed by: L'. 0, ., ;. I.:,. I. ;, ToxCalc v5.0 Page 1

CERIODAPHNIA TEST INFOI Test Name: TVA - Watts Bar Nuclear Plant (Outfall 01') Test Conducted From: 4 1 1 03 (Da) Sites/Concentrations:

1. r

4.

3 J

2.

0,l3'/

5.

3.

I.-I 6.I. Stock (if applicable): Control Water Type (/): 20% Dilute Mineral Water + Trace Minerals Other (descnbe): Dilution Water Type (V): 20% Dilute Mineral Water + Trace Minerals Other (describe): Source of Test Organisms: S&ME brood boan RMATION SHEET .)To ,b., -(Day 7)

1.

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ Er ElD/ D I nos. Age of Test Organisms: Isolated neonates for test on '- I i LJ (date) from 0 8 '5-to 21i5I (time) Initialsj3? Record of Minor Test Non-Confornity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed u (06eviewer Initials) on 4b-316I3 (Date) REV.0, January 00 (ceriodaphniltes sheet)

Ceriodaphnla Daily Test Information Logsheet TestName: TVA-WattsBar(Outfall 101) Test Dates: - 1 IL - A4 1 -e a Daily Test Temperature Information I InforInation

IChan, Wt information (cup -25+/-1°C)

Feeding Informaton Change, or Test Termination Control Water Carboy No. and Date and Incubator Temp. (C) Therm. Fed 100 uL YCT Date Fed 100 uL Selanastrum Start Time End TimeAdditional coents Initials No. YCT Selenastnim Date a.m. p.m. Carboy # Date Prep,. Day 0 f Z4 a I's 1(120qz 3 D)t4/S 0 YTZIO3___4 L.II3%t DayS O

t.

. z I___ 2/S3 K0 &0c) 3/2q/03 j4q4 pS ( 3 L1/i3/O3 A-> Os .( [ 3 lo IN 1, S6 5; 3gls1l 13t/o iw1 s 3 1S3 Day3 1`,~ as. L D s. o t ( L - a l 31 1vteD 14 LI .{L In 4i L Day4 PC 3S.O 2'&I ,- ( IS a s)^3 12& 3I3L

(4LS

)Q ) 3 DayS 32-* S 3S2j L)Sk Day5 I 5.L Vs. c/ oa1 3 25 3Igq/b3 130 132J 34Ii8/ ay D3 2' \\ 2&, I 0 1 335 a(s6s3 l 3 3 ) ~3lo3 1 3:3 i 33 3 s Day7 ( a.c0 2N.9 C

~*.* Cexiodaphnia 3-Brood Survival and Reproduaction-

1

^ Raw Data Sheet Client: TVA Watts Bar - Outfall 101 Location: Rhea County. Tennessee Analysts. 2j, sqs Test Dates: '; hI -lu+/- 3 Control 20% DMW No. of Young No. of Original Adults Replicate No. of live Young Per Init. Day 1__ = 10 G P__ 4 _ 0 44 .4 l-. 5 9_ Iol I O qit cO O J.^ 6 2 o o 0 _ c C _ _ O O kL 7 } b} 0} l1 3IS ( t1 .; 2 1 22:E 1 2S1 IG 9_5.; I e-s b '> Replicate No. of No. of live No. of Young Per l_ l 1 2 3 4 5 6 7 8 9 10 Young Adults Originai-Adults Adult Init. Day -- 1 _1 _ 10 1_1o_ (W3l 2 Io C) C q-zO 0.83% o 3 7 0 Outfall 4 l1 a 3 S I 101 _1/20 5 _O'o x4o1 -5UO ID t __\\_ ~ 6 3 L 9 1 7 \\ 121 ollyx )a C) 9Z JO Tot 2S 32 2zl 2 I M 23.8 Replicate l No. of No. of live No. of Young Per rT 1 2 13 4 5 6 7 8 9 OYoung Adults OriginalAdults Adult Init. Day - I 9 ITlD &-1 1= -1' 0 III I

1),0 I

C 1.7% 2 1 2 0 T1 I 0 0 Outfalllo 3 _O IC_ _0 101 Z'%1 4 a03C]t;lll]tG 33 l lt rbol 5 -ALA t5 l\\D l,\\I;kl 10 Cll°l C att qs q IO l 6 0 :2+1t) I1t C) o 4t c) 14-1 l- )o I 10 1Ii I7 4 I +1 3 10.12tl 3 I le "I I Cbq I C) I ) lol10w -1 s 1 ~ c1 1 c1 1 ____1 11 6TOta2 0f. _3:1242lW2T7STS11 C m Page 1 l ~~ ~. -. -,-:.s. R epl icate. No. of live Adults Young er ..Adult 2 5 6 I I 4 7 8 9 10 Wl Total 38

Client: TVA Watts Bar-Location: Rhea County. Analysts: Test Dates: J1 -4'-1 Ceriodaphnia 3-Brood Survival and Reproduction" >i" -- *-0Raw Data Sheet Outfall 101 Tennessee -=- R 2 licate = No. of No. of live No. of Young Per 1 2 3 4 5 6 7 8 9 10 Young Adults Original Adults Adult Init. Day - 10~~~~ 3.3% PP 2 eo = O Outfall ( 3 )_t 1 o 0 101 9 4 D 4 G ( 5 3 4 q t~~1 o 0 -I (~5I 6 O 24 O OIO O O JO _7 IC I Jo I 1 T ot a 23-MT-2 2T3 __ s272S;0 9lo3 I CDII

10.

s 6.6% Outfall 101 Replicate No. of No. of live No. of Young Per Init. Day-- l ___l E 0 10 10 I I 0 2 = ) _ _c_ o 3 Xt-0 J 0 0 ll4 IS I q I o 5 6 lo I I> C= 2& II I I I ~~.1. RQ Total C) 32.19Lfl 2b17 1] 2Z12i6 1 9 419 722*9 S ____________ .1. ~~ 1 .L_.,. L, I^.~ _1. ..............,. £ 'I A tkuN-En- ____1Lb\\q r>\\<. i_____ Repicat No. of No. of live No. of Young Per l 1 2 3 4 5 6 7 8 9 10 Young Adults OriginalAdults Adult Init. Day ---------- I I O CI 13.2% M 2 I> C) 1 v ll Outfall 3 I-__ 101 4 2 (oli 5lS C'::i CS q 07 I~~~ 1s it 5 1,&1, -lw<l5lllllN 1s I -3 I-s.n I I W1 6 G 1°11lQO l2 0 110 Iq 0 =- I 7 1sICo6I-IQ I 10--K 1 10 I,4 ~To2 90 32ZJd232g91 2-3 ?- Page 2 1 2 3 4 5 6 7 8 9 10 Replicate No. of Young No. of live Adults No. of Original Adults Young Per Adult 17.<1

Ceriodaphnia 3-Brood Survival and Reproduction Raw Data Sheet Client: TVA Watts Bar - Outfall 101 Location: Rhea Countv. Tennessee Analysts:,-;' Test Dates:

'41 i

-11 !a Intake Replicate No. of No. of livel No. of Young Per -F2T3 4 5 6 7 8 9101 You Aduits 1OriQinal Adnltd Ailt Init. Day--- _1 1 1O1 1 1 1 1 1 1 + o-1 0 0 2 0 ) I)II 2_ _K X 2 _0 __-__O)s 1 3

ID I 4 3 L4 (4 q s 1! q IS5 or C IW l0 O _S 5 iq 2 O 5N 5 it n 5 ?- \\0 CO --7 S~~~~~2 6 ) C9(4 Q+ qP )St O )O 4*9q 7 O O) 0 O) o 31 31t1 GV Z .6 I_I I I Total ? V 23 2S ZS 3 21 } 3 S I ( )n -v y' Page 3 1 T T~~~~~~~ f s_

a_

Ceriodaphnia Survival and Reproduction Test-Reproduction

Start Date

4/14/2003 14:00 Test ID: WB101cr Sample ID: End Date: 4/21/2003 13:50 Lab ID: - S&ME Inc. Sample Type: Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: Comments: TN0020168-NPDES Permit# EFF3-Power Plant CD-Ceriodaphnia dubia Conc-% 1 2 - 3 4 5 6 7 8 9 10 31.000 31.000 25.000 23.000 27.000 4.000 23.000 22.000 27.000 31.000 32.000 31.000 23.000 33.000 27.000 28.000 0.000 32.000 24.000 26.000 29.000 30.000 22.000 29.000 29.000 29.000 . 32.000 26.000 24.000 24.000 24.000 23.000 27.000 21.000 23.000 22.000 0.000 27.000 27.000 29.000 28.000 27.000 28.000 21.000 27.000 24.000 20.000 24.000 27.000 25.000 30.000 23.000 22.000 26.000 24.000 23.000 23.000 21.000 28.000 25.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 25.100 1.0000 25.100 0.000 31.000 36.536 10 25.400 1.0000 0.83 23.800 0.9482 23.800 4.000 32.000 32.292 10 90.50 75.00 25.400 1.0000 1.7 26.400 1.0518 26.400 20.000 32.000 14.854 10 100.00 75.00 25.400 1.0000 3.3 26.300 1.0478 26.300 23.000 33.000 12.807 10 95.00 75.00 25.400 1.0000 6.6 22.500 0.8964 22.500 0.000 32.000 37.727 10 83.00 75.00 23.850 0.9390 13.2 25.200 1.0040 25.200 21.000 30.000 13.721 10 89.50 75.00 23.850 0.9390 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.68395 1.035 -2.3437 7.25168 Bartlett's Test indicates unequal variances (p = 3.49E-03) 17.6006 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 13.2 >13.2 7.57576 Linear Interpolation (80 Resamples) Point SD 95% CL Skew Kluo ICi 0 ICi 5 IC20 IC25 IC40 IC50 o.UU.3 >13.2 >13.2 >13.2 >13.2 >13.2 >13.2 1.0* 0.9 - 0.8 - 0.7 - 0.6 - cn 0.5-o 0.4 0.a 0.3-. 0.2 - 0.1-0.0 -0.1 - -0.2 0 5 10 15 Dose % Reviewed by: Lo D-Control 0.83 1.7 3.3 6.6 13.2 7. I

1...

ToxCalc v5.0 Page 1

I ..., I Ceriodaphn_a Survival and Reproduction Test-Reproduction Start Date: 4/14/2003 14:00 TestID: WB1011ncr Sample ID: TN020168-NPDES Permit Ed Date- '4/21/2003 13:50 Lab ID: S&ME Inc. "SampleType: EFF3-PowerPlant Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: CD-Cenodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 31.000 25.000 23.000 29.000 29.000 0.000 27.000 27.000 29.000 Intake 35.000 25.000 23.000 25.000 25.000 31.000 22.000 28.000 31.000 35.000 Transform: Untransformed Conc-% Mean N-Mean Mean Min Max CV% N D-Control 25.100 1.0000 25.100 0.000 31.000 36.536 10 Intake 28.000 1.1155 28.000 22.000 35.000 17.003 10 Rank 1-Tailed Sum Critical 108.00 82.00 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.76207 0.868 -2.4231 7.96181 F-Test indicates equal variances (p = 0.06) 3.71029 6.54109 Hypothesis Test (1-tail, 0.05) Wilcoxon Two-Sample Test indicates no significant differences ToxCalc v5.0 Reviewed by: L'6 Page 1

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 4/1412003 14:00 Test ID: WB101cr Sample ID: TN0020168 End Date: 4/2112003 13:50 Lab ID: S&ME INC. SaiimleType: ' EFF3-Power Plant Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 .7 8 9 10 D-Control 31.000 31.000 25.000 23.000 29.000 29.000 0.000 27.000 27.000 29.000 0.83 27.000 4.000 23.000 22.000 32.000 26.000 28.000 - 27.000 28.000 21.000 1.7 27.000 31.000 32.000 31.000 24.000 24.000 27.000 24.000 20.000 24.000 3.3 23.000 33.000 27.000 28.000 24.000 23.000 27.000 25.000 30.000 23.000 6.6 0.000 32.000 24.000 26.000 27.000 21.000 22.000 26.000 24.000 23.000 13.2 29.000 30.000 22.000 29.000 23.000 22.000 23.000 21.000.28.000 25.000 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 25.100 1.0000 25.100 0.000 31.000 36.536 10 25.400 1.0000 0.83 23.800 0.9482 23.800 4.000 32.000 32.292 10 0.447 2.287 6.652 25.400 1.0000 1.7 26.400 1.0518 26.400. 20.000 32.000 14.854 10 -0.447 2.287 6.652 25.400 1.0000 3.3 26.300 1.0478 26.300 23.000 33.000 12.807 10 -0.412 2.287 6.652 25.400 1.0000 6.6 22.500 0.8964 22.500 0.000 32.000 37.727 10 0.894 2.287 6.652 23.850 0.9390 13.2 25.200 1,0040 25.200 21.000 30.000 13.721 10 -0.034 2.287 6.652 23.850 0.9390 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.68395 1.035 -2.3437 7.25168 Bartlett's Test indicates unequal variances (p = 3.49E-03) 17.6006 15.0863 Hypothesis Test (1 tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 13.2 >13.2 7.57576 6.65233 0.26503 22.6167 42.3167 0.74922 5,54 Linear Interpolation (80 Resamples) Point SD 95% CL Skew Kluz ICi0 IC15 IC20 IC25 IC40 IC50 O.iUU >13.2 >13.2 >13.2 >13.2 >13.2 >13.2 1.0* 0.9 - 0.8 - 0.7-0.6 - m 0.5- ° 0.4-0 0.3-0.2-0.1 0.0 -0.1- -0.2 ,Oo /° 0 5 10 15 Dose % Reviewed by: l Page 1 ToxCalc v5.0

Cerlodaphnia Survival and Reproduction Test-Reproduction StartOate: 4/14/2003 14:00 Test ID: WB1OlIncr Sample ID: TN0020168 End Date: 4/21/2003 13:50 Lab ID: S&ME INC. SampleiiType: EFF3-Power Plant Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: CD-Ceriodaphnia dubia Comments: Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 31.000 31.000 25.000 23.000 29.000 29.000 0.000 27.000 27.000 29.000 Intake 35.000 25.000 23.000 25.000 25.000 31.000 22.000 28.000 31.000 35.000 Transform: Untransformed Conc-/0 Mean N-Mean Mean Min Max CV% N D-Control 25.100 1.0000 25.100 0.000 31.000 36.536 10 Intake 28.000 1.1155 28.000 22.000 35.000 17.003 10 1-Tailed t-Stat Critical MSD -0.888 1.734 5.666 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.76207 0.868 -2.4231 7.96181 F-Test indicates equal variances (p = 0.06) 3.71029 6.54109 Hypothesis Test (1 -tail, 0.05) Homoscedastic t Test indicates no significant differences Y. ~ 0=_o-.' /C Reviewed by: = Page 1 ToxCalc v5.0

Fish Water Chemistry WB101-April 14-21,2003 Initial Chemistry Fish - Control INITIAL AND FINAL WATER CHEMISTRY (FATHEAD) 0 1 3 -4 -- 5 6 MIN .M MEAN"-' Temp 25.6 25.4 25.4 24.2 25.3 25.8 25.0 24.2 - 25.8 i,\\25.2 DO 7.2 6.7 7.0 6.7 6.6 6.6 6.2 6.2 _ 7_ 2 l6.7 pH 8.0 7.9 8.1 8.0 7.9 8.1 8.1 7.9 - 8.1 80 Cond 400 377 383 387 388 392 401 -377 401 = - '4390 Hard 100.0 98.0 96.0 96.0 96.0 80.0 80 100 94.31 Alk 61.0 63.0 60.0 63.0 64.0 64.0 60 64 = 62.5 Concentration 0.83% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.7 25.4 25.6 24.3 25.4 25.8 25.1 24.3 25.8 25.3 DO 7.1 6.6 7.0 6.7 6.6 6.4 6.2 6.2 7.1 _ 6.7 pH 8.0 7.9 8.1 8.0 8.0 8.1 8.1 7.9 8.1 = 8.0 Cond 380 375 386 386 389 391 399 375 399 _ 387 Concentration 1.7% 0 1 2-3 4 5 6 MIN MAX MEAN Temp 25.6 25.4 25.5 24.3 25.4 25.8 25.2 24.3 25.8 25.3 DO 7.0 6.6 7.0 6.6 6.6 6.4 6.3 6.3 7.0 6.6 pH 8.0 7.9 8.1 8.0 8.0 8.2 8.1 7.9 8.2 8.0 Cond 376 373 384 384 386 392 397 373 397 _ 385 Concentration 3.3% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.8 25.4 25.3 24.3 25.6 25.8 25.3 24.3 25.8 25.4 DO 7.1 6.6 7.0 6.7 6.5 6.2 6.2 6.2 7.1 1 6.6 pH 8.0 7.9 8.1 8.0 8.0 8.2 8.2 7.9 8.2 = 8.1 Cond 372 369 381 380 383 388 393 369 393 _ 381 Concentration 6.6% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.6 25.5 25.3 24.3 25.6 25.9 25.3 24.3 25.9 25.4 DO 7.0 6.6 7.0 6.7 6.5 6.4 6.3 6.3 7.0 6.6 pH 8.0 8.0 8.1 8.0 8.0 8.2 8.2 8.0 8.2 8.1 Cond 367 363 373 373 378 382 387 363 387 375 Concentration 13.2% 0 1 2 3 4 5 6 MIN MAX MEAN _ - S o j _ ^=s _= n _ j A b l = nl _ TM 25.4 25.3 25.4 24.3 25.7 25.8 25.3 7.1 6.6 7.1 6.8 6.5 6.3 6.3 8.0 8.0 8.1 8.0 8.0 8.2 8.2 369 350 368 361 366 374 374 6.3 7.1 _ 6.7 8.0 8.2 8.1 350 374 j 366 Page 1 Temp DO pH Cond -1.:, -.:. :

,

, --, ,, !, :, -, -; -: - -. , -i. "., 1, -..,..,.,, i-, .. I vI Z4.3~ 25.8 2-5

Fish Water Chemistry i u,... Concentration 1 00% Temp DO pH Cond Hard Alk TRC 0 -- 1 -: 2 3 25.4 25.1 7.8 7.9 .8.1 7.9 181 178 84.0 65.0 0.05 25.2 7.6 8.1 164 70.0 60.0 0.07

4...

4 5 24.2 7.7 8.0 - 171 . 24.8 7.0 8.0 197 80.0 60.0 0.05 6 MIN 'MAX

MEAN, 25.9 25.4 6.8 6.7 8.0 8.0 203 190 6.71 7.9r

'1 64 " , -- 70r -. i'-.60J l., 0.05- -'25.9 2, 5l1 .I 251l ., 7 9 _74 8.1 -8.0 203 183 r 84 - -. TT-7'T78

065 61.7.

.0.07 A 0.06 0 1 2 3 4 5 6 MIN MAX 24.9 7.7 7.8 158 76.0 58.0 0.06 25.4 7.8 7.8 159 25.4 7.4 7.8 154 7.4 8.9 _ 8.1 7.8 8.1 7.9 154 165 159 68 76 73.3 54 59 57.0 0.04 0.07 0.06 Final Chemistry FISH Fish - Medium 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.5 25.3 24.8 25.6 25.0 24.8 24.8 24.8 25.6 l 25.1] DO 6.3 6.0 5.2 5.6 5.7 5.5 6.3 5.21 6.31 5.8 pH 7.9 7.9 7:6 7.3 7.4 7.6 7.7 7.3[ 7.91r 7.6 Concentration 0.83% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.3 24.8 24.5 25.0 24.9 24.5 24.5 25.3_ 24.9 DO 6.5 5.7 5.3 5.3 5.8 5.4 6.3 5.3 6.5 5.8 pH 7.9 7.7 7.6 7.2 7.6 7.7 7.6 7.21 7.9 _79 7.6 Concentration 1.7% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 25.2 24.9 24.6 25.0 25.1 24.8 24.6 25.2 25.0 DO 6.5 5.6 4.9 5.0 5.5 5.4 6.3 4.9j 6.5 5.6 pH 7.8 7.7 7.5 7.3 7.5 7.6 7.7 7.3 7.8 l 7.6 Concentration 3.3% 1 2 3 4' 5 6 7 MIN MAX MEAN 25.5 25.9 24.9 24.7 25.3 24.9 24.8 6.1 5.6 5.0 4.9 5.5 5.3 6.2 7.7 7.7 7.5 7.3 7.6 7.7 7.7 24.7 25.91 4.91 6.21 1 5.5 7.31 7.7 7.6 Page 2 -- 24.2 Intake Temp DO pH Cond Hard Alk TRC 25.2 8.4 7.9 162 25.7 7.8 8.0 165 76.0 59.0 0.04 24.0 8.6 7.8 154 25.3 8.9 8.1 163 68.0 54.0 0.07 MEAN Temp DO pH g E I 24.C()I I 25.71 25.1 25.1

Fish Water Chemistry z - I Concentration 6.6% 1 2 ,3 4 .- 5 6 MIN MAX -MEAN Temp 25.0 25.6 24.7 24.6 25.2 24.7 24.3 24.31 25.6 _J.- 24.9 DO 6.2 5.8 5.0 4.9 5.5 5.4 6.4 .9 61 6.4 5.6 pH 7.8 7.7 7.5 7.3 7.6 7.5 7.8 7 7 . 7.6 Concentration 13.2% 1 2 3 4 5 6 7 MIN - MAX ": MEAN, Temp 24.8 25.6 24.7 24.6 25.3 24.8 24.1 24.1 25.6 = 24.8 DO 6.4 5.4 5.0 4.9 5.1 5.2 6.5 [ 4.9 6.5 _ 5.5 pH 7.7 7.7 7.5 7.3 7.5 7.6 7.7 7.3 7.7 _ 7.6 Intake 1 2 3 4 5 6 7 MIN MAX MEAN Temp DO pH 24.8 25.0 6.3 6.4 7.7 7.7 24.6 5.3 7.5 25.1 5.4 7.5 25.1 5.8 7.7 24.9 5.3 7.7 25.3 6.7 8.0 t I Test Temperature Mean 25.1 Minimum 24.0 Maximum 25.9 Word Review by: (2rz 6 2o? Excel Reviewed by&-.z - S,-0 2-ccVS 5.31 6.71 1 5.9 7.51 8.01 1 7.7 Page 3 % 1-I..... ..1. I I z v.r,,. 24.6 2531 25.0

Client: TVAWattsBar'-Outfall O1 Location: Rhea County. Tennessee Template No.: F M 7 C Test Type: Fathead Minnow 7-day Chronic: Analysts: Dates/times: I / Age ofLarvae: -_-_____;__-_-____ D a Control: Mod. Hard O 1 2

3

-4 5 ¢6 Remarks Temperature-Initial q? aS .q.aIa -293 as.6 2O Final _2 3a S 2. D.O. Initial

9.

i r 6 .(B (. _o __ Final 6 3 -. o S. I_ S 3-I pH Initial ) I1 \\q -\\ 5i. O Final - 1.9 1 le -. (D Alkalinity _e1 ( 1_ 6 Hardness I I = C Conductivity Hoo Mm 3 3 ( Analyst Initials . o Q o 2 Y (M QA Review Initials 0 O S Day 0.83% Outfall 101 0 1 2 3 4 5 6 Remarks Temperature-Initial 1 a7 Q5 42SA 2 X o - CA r It aX I Final 2 S 4 .3 4.S 2 Xs-q C D.O. Initial l . o /L. a lZ tt CA Final _l 6 5. S* 5.3 _c pH Hitial . O s \\

o O

Final -1 -I. e Conductivity 3IM 3L 9 1__ Analyst Initials r-<'r QA Review Initials $K o Day 1.7% Outfall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial

5. 1 5

tS is 2S 2 s -= Final I asa a a(; as.p o Z5 1 D.O. Initial .Io_ 1,(O

7.

L-(O. Final _s -c S. _.5 S_._ pH Initial 'all n Final j 1 .-S ,S Conductivity 313. tc Analyst Initials F S{ P. r QA Review Initials I I Pu I SE -V AC:o dcL-3 tcv C ot fC-v 4-1 4-o C.<o s?. -- C Y-Lt-0 4 Aoer1 s `2 as, -AC 5,ir\\ L O, 4 j cLcs ~4.c Ie,C-S ocA

Client: TVA Watts Bar - Outfall 101 Location: Rhea County, Tennessee Template No.: A Test Type: Fathead Minnow 7-day Chronic Analysts: V 1'r,-, r.v. Dates/times: Z 1l .ge of Larvae: sa 3.3% Oufl 10 ~Davj -z 3.3% Outfall101 ° 2 3 4 5 6 7 Remarks Temperature-Initial QSJ4 Q5.3 U 6 3- __as3 Final 2 5 g s9. *.q gq7 a 5.3 Z4,5 a D.O. Initial r,7. Q,a-- Final 6.I O tI. 5-OL _q S 1S-3 pH Initial 0

1.

_8-Z_ Final _1.L t'1 Conductivity s % 80 _3_3 39 Analyst Initials %SU o b QA Review Initials S Day 6.6% Outfall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 25 -S4. 2 'S _A. Final 2s. 0 &Y I 7.V . R D.O. Initial G. 1 ( O -I ki 1 6 4 5 C 3 Final 62..

3. S.o 4.q

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L. pH Initial 6 O 5 l O Final Conductivity 3(9 3 l3______3_ Analyst Initials er ,h r o r QA Review Initials Day 13.2% Oufall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial S5* q Uj. £.>. S 2*3 Final 2, .4-8 aS. aq.c N zq.S D.O. Initial ( Final 4 Is-s 4{9 5 pH Intial 6-6 ii.b Ž ___A_ Final 1.1 j Conductivity 249 n -A 6 :a, 3I ?Y i Analyst Initials / o ff QA Review Initials 9 _______Q_ l X - w !1 % / j .I. I.. i;

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-I... Client: TVA Watts Bar - Outfall 101 Location: Rhea County Tennessee Template No.: I_ Test Type: Fathead Minnow 7-da Chronic .Analysts: P ._ -a Dates/times: i,,ll PA I q Io Age of Larvae: = 4 Day 100% Outfall 101 0 1 2 3 4 5 6 Remarks Temperature-Initial Q. j S ; 4 S a _9 _4 Final D.O. Initial 9 n 1 C (.c4-,-o. D Final __/ pH Initial 8 1 1 .o Final Alkalinity Z &O Hardness Rq 17 A Conductivity I __I __IA l>k \\n 1 qW aO Residual Chlorine 0.c a t> 07 O Sample Date 1 14 u; 4t1 Ai I Analyst Initials 1 j c QA Review Initials v -o = Da ___Da Intake 0 1 2 3 4 5 6 7 Remarks Temperature-Initial laz 'g q -q -1 ____a_S Final E.0.lAr .)5. Cr¢ D.O. Initial 9 C.V 9 4 A. C_£ !c Final 6.3 5 -3 5.7 T; 4c r \\ 4 o pH Initial W 19-2q-b-. a! Final

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Alkalinity 5 9 StA Hardness I o (sR ( / Conductivity [I S Iq Residual Chlorine / O. n Z O Sample Date il 'l -41ILi '.1 4 I I 1 4116 ' N11 Analyst Initials ( 5 %g P r s QA Review Initials 2 Lk, -(o _P p / 4 ) ; I)as 2, L%I r O ( - fy-, - a-Z -S 0-"t-Lu -CCY-t L-> "-.,: " -* -3., - 0. Z. 7. -j O" S" r-z' P,, to tb-", = VAI

Cerio Water Chemistry WB1 01 April 14-21.2002 INITIALANDFINALWATERCHEMISTRY (CERIO) Initial Chemistry CERIO Control 0 1 2 3

4 5

6 MIN MAX MEAN Temp 25.5 25.4 25.4 -24.2 25.8 25.8 25.1 24.2 25.8-_ t-25.3 DO 6.9 6.7 7.0 6.7 6.7 6.5 - 6.5 6.5 -7.0 6.7 pH 8.0 8.0 8.1 8.0 8.0 8.0 8.1 -8.0 8.1 _ 8.0 Cond 166 169 167 167 166 175 163 163 175 168 Hard 80.0 82.0 80.0 80.0 82.0= 80.7 Alk 58.0 59.0 62.0 58.0 62.0 59.7 Concentration 0.83% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.5 25.3 25.4 24.2 25.8 25.7 25.1 24.2 25.8 25.3 DO 6.9 6.6 6.8 6.7 6.7 6.7 6.3 6.3 6.9 6.7] pH 8.0 8.0 8.1 8.0 8.1 8.1 8.1 8.0 8.1l 8.1 Cond 167 164 169 168 168 167 164 164 169l 167 Concentration 1.7% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.5 25.4 25.5 24.3 25.7 25.8 25.2 24.3 25.8 25.3 DO 7.0 6.6 6.9 6.7 6.7 6.8 6.2 6.2 7.0 6.7 pH 8.0 8.0 8.2 8.0 8.1 8.1 8.2 8.0 8.2 8.1 Cond 167 165 169 168 169 169 165 165 169 167 Concentration 3.3% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.4 25.4 25.5 24.3 25.7 25.4 25.2 24.3 25.7 25.3 DO 7.0 6.7 6:9 6.7 6.7 6.8 6.2 6.2 7.0 6.7 pH 8.0 8.0 8.1 8.0 8.1 8.1 8.1 8.0 8.1 8.1 Cond 167 169 168 168 169 170 165 165 170 168 Concentration 6.6% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 25.4 25.3 25.5 24.5 25.6 25.6 25.2 24.5 25.6 25.3 DO 7.0 6.6 6.9 6.7 6.7 6.7 6.3 6.3 7.0 6.7 pH 8.0 8.0 8.1 8.0 8.1 8.1 8.1 8.0 8.1 8.1 Cond 168 169 168 169 170 171 166 166 171 _ 169 Concentration 13.2% 0 1 2 3 4 5 6 MIN MAX MEAN Temp DO pH Cond 25.4 25.3 25.0 24.5 25.6 25.6 25.3 7.0 6.6 7.1 6.7 6.6 6.6 6.3 8.0 8.0 8.1 8.0 8.1 8.1 8.1 168 170 167 169 172 174 167 6.3 7.1 l 6.7 8.0 8.1 8.1 167 174 170 1, ,.Aw, Page 1 of 3

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Cerio Water Chemistry Concentration 100% Temp DO pH Cond Hard Alk TRC Intake Temp DO pH Cond Hard Alk TRC 0 .1 - 2 3 4 5 6 25.5 25.4 25.1 24.6 24.8 25.8 25.: 7.9 7.9 7.8 8.1 7.0 6.8 - 6.' 8.1 7.9 8.1 7.9 8.0 8.0 8. 181 - 178

161 171 197 - 203 17:

84.0 70.0 80.0 65.0 60.0 60.0 0.05 0.07 0.05 0 1 2 3 4 5 6 25.3 25.2 7.8 8.4 8.0 7.9 165 164 76.0 59.0 0.04 25.0 24.1 24.9 25.7 25.6 8.8 8.7 7.7 7.8 7.2 8.1 7.8 7.8 7.8 8.5 163 155 158 159 149 68.0 76.0 54.0 58.0 0.07 0.06 MIN MAX" .MEAN 2 24.6 25.8 9 6.8 8.1 7.5 1 7.9

- 8.1 t

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161 :" 203 180 - 70 ---%84 _

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,r i4_ .-,.0 i:s-65 6ti .- 6.7 7. t. 0.05 -. >0.07 I 0 Th ,.,06 MN MAX MEAN 7.2 8.8 1 8.1 7.8 8.5 1 .C 149 165 159 68 76 73.: 54 59 57.C 0.04 0.07 - 0.0E Final Chemistry CERIO Cerio - Medium 1 2 3 4 5 6 7 MIN MAX Temp 25.8 25.0 24.8 24.5 25.5 24.8 24.0 24.01 25.81 1 24.9 DO 6.3 6.0 6.4 6.6 6.3 5.5 6.4 5.51 6.6l 6.2 pH 7.9 7.6 8.1 7.9 8.0 8.3 7.8 7.61 8.31 l 7.9 Concentration 0.83% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.6 24.9 24.7 24.5 25.5 24.9 24.1 24.11 25.61 24.9 DO 6.3 6.0 6.3 6.5 6.2 5.5 6.4 5.51 6.5 1 6.2 pH 8.1 7.7 8.2 8.0 8.0 8.2 8.0 i7.7l 8.2l 80 o Concentration 1.7% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.5 24.9 24.7 24.4 25.5 25.0 24.1 24.11 25.51 24.9 DO 6.4 5.8 6.3 6.5 6.2 5.5 6.4 5.5 6.5 6.2 pH 8.0 7.7 8.2 8.1 8.1 8.3 8.0 7.71 8.3 8.1 Concentration 3.3% 1 2 3 4 5 6 7 MIN MAX MEAN Temp DO pH 25.5 25.2 24.8 24.4 25.4 25.0 24.2 6.5 5.8 6.3 6.5 6.0 5.5 6.5 8.2 7.7 8.2 8.2 8.2 8.2 8.0 Page 2 of 3 MEAN I 24.9 24.21 25.51 5.51 6.51 1 6.2 7.71 8.21 T 8.1 24.11l 25.7 25.1

Cerio Water Chemistry I I

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~.

I . v I.IX Concentration 6.6% 1 2 3 4 5 6 7 MIN MAX .MEAN:-- Temp 25.5 25.1 24.8 24.3 25.4 25.0 24.2 24.2 24.9 DO 6.6 5.8 6.4 6.6 6.0 5.5 6.5 5.5 6.6 6.2 pH 8.1 7.7 8.1 8.2 8.2 8.2 8.2 7.71 -8.21 ---I. Concentration 13.2% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.5 25.3 24.8 24.3 25.5 25.0 24.3 24.3 25.51 25.0 DO 6.8 5.8 6.3 6.6 6.0 5.5 6.5 5.5 6.8 _ -6.2 pH 8.1 7.7 8.1 8.1 8.1 8.2 8.1 7.7 8.21 8.1 Intake 1 2 3 4 5 6 7 MIN MAX MEAN ~~~~~~~~~~~~~~~~~~2.2 2.2 2.8_.. Temp DO pH 25.1 25.2 24.9 24.2 24.9 24.9 24.3 6.6 6.0 6.3 6.3 6.4 5.8 6.5 8.2 7.7 8.2 8.3 8.1 8.3 8.3 Test ter Mean: 25.1 Min: 24.0 Max: 25.8 Word Review by: CS -6C2 - 206 LI Excel Reviewed by: Cl-t 6-6 - 202 Page 3 of 3 5.8 6.6 _ 6.3 7.71 8.3 8.2 I .j o-d !.7 W

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V.-. ,~ Client: TVA Watts Bar-Outfall 101 Analysts: uD, 77 Location: Rhea Count_. Tennessee Dates/times: 4, I l t Template No.: -_I_-_ -_-_-_i_Age of Neonates: o' Test Type: Ceriodaphnia dubia 7-dav Chronic Day Control: 20% D 1 2 3 4 5 6 Remarks Temperature-Initial 25 54 24 Final S. 2co . t S - a_ D.O. Initial LI o*1 @3 li0 _S Final 6 .0 (. t 4 _o pH Initial __S____ Final 89 1 D ALkalinity : ;s _ Hardness 9; Conductivity 1(3j i Analyst Initials ,o I p-, - QA Review Initials I 1j S S D x __ay 0.83% Outfall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial

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3.

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8.5 Conductivity Iu1 \\u_ I_ A I( Analyst Initials l qA St M .&2. QA Review Initials l S__ I I I.. .1 -. -

Client: TVA Watts Bar - Outfall 1 .Analysts: pq-: - Location: RheaCounty.Tennessee -, Dates/times:,. i1Lj11q Mw)6-Template No.: I Age of Neonates: -s!s-Test Type: Ceriodaphnia dubia 7-dav Chronic D ay = 3.3% Outfall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial QS. 4 S;t 55 25, 5.- Final 2S.&& d'8MO2 2 D.O. Initial fl.O (vj1 &9 Ls 7J -I_ C__ Final S. G S b (o.- pH Initial s O.O I S O 1 4 Il Final _ B. : 7 n a.; S. Conductivity lu( I q 1Li 'i,S Analyst itials p - n QA Review Initials fl Da 6.6% Outfall 101 0 1 2 3 4 5 6 Remarks Temperature-Initial a. S"5 Q ss 2S.- _o _____s_ Final aS. 2L' L ;5.t D.O. Initial ') I I.-D S Final _ ? (JG .O - (o.& pH Initial

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Day 13.2% Oufall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial aQSS.3 .p O 29.& IS.43 Final 25

2. 3

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Client: TVA Watts Bar - Outfall 01 Analy Location: Rhea County. Tennessee Dates) Template No.: I Age of Test Type: Ceriodaphnia dubia 7-day Chronic sts: - ED, A otimnes: <, to SNeonates: -h Day 100% Outfall 101 0 1 2 3 4 5 6 7 Remarks Temperature-Initial S. S ,* j o S.6 Final __/ D.O. Initial 1 2 oi .0 f3.9 Final l pH Initial -. 9 t1 q 8 ' V 4:i Final_// Alkalinity I /U <1fi Hardness -I 10 8 Conductivity 1i1 I1 2 ot o Residual Chlorine o.oS /: _ Z Sample Date __i 4Au 4L' 4 _1 _ _ 4118 Analyst Initials P)O b 3-Pj I A0,l QA Review Initials Pc I v 54a Day Intake 0 1 2 3 4 5 6 Remarks Temperature-Initial a s ci. 2q 1 .q Final _12___J 1_ 2. D.O. Initial .l5 1 -T. Final 6NbG 3. h pH Initial ____a___ Final I. 1.7 3 Alkalinity 59 c 5q Hardness I ( Conductivity I 5 1 Residual Chlorine o.0* C olI Sample Date bt\\ A ,ill -ills tLjj '41I-Analyst Initials r, 2 I QA Review Initials CW r I $ 1 0 =- i p.c. C C4'-tr -i 1 3 h,.D v7) -..I

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Watts Bar Nuclear Plant Biomonitoring April 14 - 21, 2003 Appendix D Reference Toxicant Tests and Control Chart Information

-~.

SE - Fathead Chronfc Control Chart

-~E- .Updated April 21.2003 Control Growth KCL Toxicity Test ,, -g,, _, ' i Control Chart (PP) , I ,.'4'. :! Mean S 2S Mean-2s Mean+2S S&ME 09/18/01 0.7039 S&ME 10/30/01 ->0.8997 0.8018 0.1385 0.2769 S&ME 11/18/01 0.7502 0.7846 0.1023 0.2047 S&ME 12109/01 0.7514 0.7763 0.0852 0.1704 S&ME 01/29/02 0.8314 0.7873 0.0778 0.1556 S&ME 02/28102 0.7485 0.7808 0.0714 0.1427 S&ME 03/12/02 0.5773 0.7518 0.1008 0.2016 S&ME 04/02/02 0.7327 0.7494 0.0936 0.1871 S&ME 04/30/02 0.7482 0.7493 0.0875 0.1750 S&ME 06/18/02 0.5319 0.7275 0.1074 0.2148 S&ME 07/16102 0.6690 0.7222 0.1034 0.2068 S&ME 08/05/02 0.6990 0.7203 0.0988 0.1976 S&ME 09/09/02 0.8100 0.7272 0.0978 0.1956 S&ME 10/08102 0.8720 0.7375 0.1016 0.2033 S&ME 11/19/02 0.7320 0.7371 0.0980 0.1959 S&ME 12/03/02 0.8230 0.7425 0.0970 0.1941 S&ME 01/22/03 0.9200 0.7530 0.1033 0.2067 S&ME 02/11/03 0.8480 0.7582 0.1027 0.2055 S&ME 03103/03 0.7914 0.7600 0.1001 0.2003 S&ME 04/08/03 0.8020 0.7621 0.0979 0.1958 0.5249 1.0787 0.5799 0.9893 0.6059 0.9467 0.6318 0.9429 0.6381 0.9236 0.5502 0.9534 0.5623 0.9365 0.5742 0.9243 0.5127 0.9423 0.5154 0.9290 0.5226 0.9179 0.5315 0.9228 0.5342 0.9408 0.5412 0.9331 0.5484 0.9366 0.5463 0.9597 0.5528 0.9637 0.5597 0.9602 0.5663 0.9579 S&ME-Fathead Chronic Control Chart 1.1 0.9 l H 0.7-i 0.5 0.3 Te t Tests Date Page 1 Source Date IC25 CV%

17.3 13.0 11.0 9.9 9.1 13.4 12.5 11.7 14.8 14.3 13.7 13.5 13.8 13.3 13.1 13.7 13.5 13.2 12.8

-U C25 + -Mean Mean-2s -*-Mean2S 'I- .1 f 1,, -- ., f -.. wI I

Potassium Chloride Chronic Reference Toxicant Control Chart for Fathead Minnow Test date 7-d C25 (g/L KCI) 09/18/01 0.7039 10/30/01 0.8997 11/18/01 0.7502 12/09/01 0.7514 01/29/02 0.8314 02/28/02 0.7485 03/12/02 0.5773 04/02/02 0.7327 04/30/02 0.7482 06/18/02 0.5319 07/16102 0.6690 08/05/02 0.6990 09/09/02 0.8100 10/08/02 0.8720 11/19/02 0.7320 CT (g/L KCI) 0.80 0.78 0.78 0.79 0.78 0.75 0.75 0.75 0.73 0.72 0.72 0.73 0.74 0.74 using Moderately Hard Synthetic Water S Control Limits SA.75 Warning Limit 0.14 0.10 0.09 0.08 0.07 0.10 0.09 0.09 0.11 0.10 0.10 0.10 0.10 0.10 CT-2S CT+2S 1.08 0.99 0.95 0.94 0.92 0.95 0.94 0.92 0.94 0.93 0.92 0.92 0.94 0.93 0.52 0.58 0.61 0.63 0.64 0.55 0.56 0.57 0.51 0.52 0.52 0.53 0.53 0.54 s CT - SA.75 CT + SA.75 0.36 0.35 0.35 0.35 0.35 0.34 0.34 0.34 0.33 0.32 0.32 0.33 0.33 0.33 0.44 0.43 0.43 0.43 0.43 0.41 0.41 0.41 0.40 0.40 0.40 0.40 0.41 0.41 1.16 1.14 1.13 1.14 1.13 1.09 1.09 1.09 1.05 1.05 1.04 .1.05 1.07 1.07 SA.90 Control Limits CT - SA.90 CT + SA.90 0.50 0.49 0.48 0.49 0.48 0.47 0.46 0.46 0.45 0.45 0.45 0.45 0.46 0.46 0.30 0.30 0.29 0.30 0.30 0.29 0.28 0.28 0.28 0.27 0.27 0.28 0.28 0.28 1.30 1.27 1.26 1.28 1.26 1.22 1.21 1.21 1.18 1.17 1.17 1.18 1.19 1.19 16 12/03/02 0.8230 0.74 0.10 0.55 0.94 0.33 0.41 1.08 0.46 0.28 1.20 0.13 17 01/22/03 0.9200 0.75 0.10 0.55 0.96 0.34 0.41 1.09 0.47 0.29 1.22 0.14 18 02/11/03 0.8480 0.76 0.10 0.55 0.96 0.34 0.42 1.10 0.47 0.29 1.23 0.14 19 03/03/03 0.7914 0.76 0.10 0.56 0.96 0.34 0.42 1.10 0.47 0.29 1.23 0.13 20 04/08/03 0.8020 0.76 0.10 0.57 0.96 0.34 0.42 1.11 0.47 0.29 1.23 0.13 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the IC25 values. SA.75 = Standard deviation corresponding to the 75'f percentile CV. SA,75 = 0.45, as determined by USEPA for the method and endpoint.; SA.90 = Standard deviation corresponding to the 90'" percentile CV. SA.90 = 0.62, as determined by the USEPA for the method and endpoint CV = Coefficient of variation of the IC25 values. USEPA. 2000. Understandinlg and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cinicinnati, OH. Test N umber 2 3 4 5 6 7 8 9 10 I1 12 13 14 15 CV 0.17 0.13' 0.11' 0.10 0.09 0.13 0.12 0.12-0.15 0.14 0.14 0.13. 0.14 0.13 I I I:

Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Fathead Minnows using Moderately Hard Synthetic Water Test date Control Control Mean CV CT MSD PMSD Survival Growth (%) (dry weight/larvae) (%) ~(mg) for Control (%) Growth CV (%) tz te-85W%t s e # X + t t f s ti s

- l:

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zW' '4 ' ' ' -' : =. -L 1-w CT (%) for PMSD (%/) I 09/18/01 2 10/30/01 3 11/18/01 4 12109/01 5 01129/02 6 02128/02 7 03/12/02 8 04/02102 9 04/30/02 10 06/18/02 11 07/16/02 12 08/05/02 13 09/09/02 14 10/08/02 15 11/19/02 16 12/03/02 17 01/22/03 18 02/11/03 19 03/03/03 20 04-08-03 95 97.5 100 100 100 97.5 92.5 95 100 97.5 100 90 100 97.5 92.5 95 97.5 92.5 97.5 90 0.839 0.705 0.850 0.493 0.669 0.723 0.662 0.813 0.460 0.696 0.338 0.538 0.508 0.715 0.674 0.504 0.403 0.555 0.509 0.434 7.5 10.1 8.6 12.3 8.1 9.9 8.0 10.4 6.7 9.2 8.0 6.2 6.0 11.0 13.7 7.4 7.0 8.1 5.1 10.9 0.1769 8.8 0.147 8.7 0.1188 9.6 0.08 9.3 0.1115 9.4 0.2536 9.2 0.1316 9.4 0.1757 9.1 0.0789 9.1 0.1965 9.0 0.1407 8.8 0.1531 8.5 0.1542 8.7 0.1988 9.0 0.087 8.9 0.153 8.8 0.1422 8.8 0.1567 8.6 0.0673 8.7 0.1357 Test number 21.1 20.9 14.0 16.2 16.7 35.1 19.9 21.6 17.1 28.2 41.6 28.5 30.4 27.8 12.9 30.4 35.3 28.2 13.2 31.3 21.0 18.6 18.0 17.8 '20.6 20.5 20.7 20.3 21.1 22.9 23.4 23.9 24.2 23.5 23.9 24.6 24.8 24.2 24.5 r

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j 8 , I j ,9.n i -/, )RMATION X 9 1i.Sl63 (Day7) Stock (if applicable): Control Water Type (1: Moderately Hard Water + Trace Minerals Other (descnbe): Dilution Water Type (/): Moderately Hard Water + Trace Minerals Other (describe): Source of Test Larvae (/): S&ME Other (describe): Date Larvae Hatched: -l5*-qlas_ Record of Minor Test !4on-Conformity Er H1T 7 - nils ) Hatch Time: -i.5 - 6'31 2 Initials: K Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed By: Y i)I Ie(Reviewer Initials) on _ (Date) REV.OI, JANUARYOO (fathead test info) ,J

it.

Ir Fathead Minnow Daily Test Information Logsheet est Name. Chronic Reference Test Test Dates: Y 8 -

no Date Daily Test Ternperature Information Feeding Information chage, oTt information (25410 C) (feeding interval 6-lh) Terminaon Control Water Carboy No. and Date and Incubator Temp. No. Fed 100 uL Feedmg Additional comments CIQ Them. Brine Feedng t Tim End Time Initials No. time a.m. p.r. Shinp Carboy # Date Prep. DayOP 2 o anc I n s 13o 13QV

2.

4q/q3 Day V at l 00 5 '4 L,: I L-. PMv:,, s o GL 10 IS ',A 2)/524Fr 10 2-S .I Z V p )SOSOq519 1 32 5 /9l63 Day 4 X.J arn pm: 11o6 NI ca 1S__ ay 3 am: I & J3c 151 Day4 am: I CO Day 6 e _ _g 1 I4ist 0 s $oi-.3ic~ 3S 2 /lb I I I O;

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Reference - Analyst: ratory . Dates: t c-s A1ULATIVE SURVIVAL OF P. PROMELAS IN CHRONIC TEST LICATE REPLICATE DAY A B C D CONC. Initials NO. A B m 1 {I 1r I 1 jn I4fl m1rXp 2 I 16 7 es I 1 e_ Control AK 3 .JC). . Ja 7 J2..lo Mod.

k 4

to kt s Hard _r 5 10 Lo 4_._ &* [i 6 J I I 2 0 i.0 O12 g JQi I... O O 250 mg/L 3 Cl I I O IQO (KCI) 4 ko 5 \\ 6 I L 1 7 0 Ci c 07%r 1 .j )) IC (D 2 O ) 16 - 500 mg/L 3 I C. 0 i f n (KCI) i 4 5 CA kc o 6 Ic E 7 ( f 4)b:b 1 I O S 2 3 t j II 750 mg/L 3 3 g 9.E (KCI) ly 4 1% 5 5 6 7 9 4 I 9r 1 I V-a1<R 2 Ct 10 XO 52 3 5c;t n 1O 1000 mg/ 4 k': (KCI) 5 .'3 __7 -; 7 6

1' 2/f J

toL~ 1500 mg/L 3 O O (KCI) J'- 4 C CD .y-5 c 0 o At6 S O O C) 7 O C) C) f -F 4 2 =_______ 5 =6 7 2 3 7 4 5 6 7 2 32 43 54 6 7 7-F 2 32 43 54 6 5_ _ __6 7F Client: S&ME Chronic Location: S&ME Labor CUN - REP DAY CONC. Initials NO.

FATHEAD MINNOW SI RAWbA SURVIVAL AND Client: S&ME Reference Test Test Conducted By: r . j:r, Date Weighed '4 Iu Lu EVEN DAY TEST TA GROWTH Oven Temp Test Dates: 'i 1% -41i5 (3 (

C)j Comments

Began drying (date/time) 41leS3h End drying (dateltime) _ufir..

Live
Original Rep.

Fish Fish Pan Tare Weight (mg) Dry Weight Pan + Fish (mg) Groups Dry Weight (Pan + Fish minus Tare) (mg) .;~,. Mean DryWeight (mO) Control A 1 D maq.4C) I .1q a

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3 B 10 t Vc

t.

a. "6b &3 C .14q .D laI t \\I. 00td\\.3;9 .( L 6 250mg/L A 10 _____9 B (0 t \\_._I___ 3.*,) c A 10 s s _s D ( 0 __a_ 3 .o D 500 mg/L A ^1 l Oc*- -I-S-- Iw \\8 a 3 3 o.b B l OQ l3 l 3 tCro.cfl>2O.> 5 o 1 IL rlt~ o .p ,a.-aL;3.9 750 mg/L A 5¢10 jswll a-V13 2.N B lo l> l .S \\m .3 t:;- Sh _______D 1 ia1cl.1@ vmTh O' 3.'2 03 1000 mg/L A JL tOIAO 9 21 3S. 3 O.?-l B 1 10 mOla .4\\ \\w su _______9 C .1 10 jgyt3.~~ to-130_______ f).5 D I JC lc3to tjj Q.W ( Exposure & Repl.

Larval Fish Growth and Survival Test-7 Day Survival Start Date:. 4/8/03 13:00 Test ID: rel'0403fs Sample ID: REF-Ref Toxicant End Dakte: 411/03 13:00 Lab llD:' S&ME Simpie Type: KCL-Potassium chloride Sample Date: Protocol: EPAF 91-EPA Freshwater TsSpce: -PPmphlsrmeas Comments:- I..anC-mg9L 1 4 0-Control 0.9000 1.0000 0.9000 0.8000 250 0.8000 0.8000 0.9000 0.9000 500 0.7000. -1.0000 0.8000 0.7000

750, 0.8000

,0.9000

..0.6000 0.7000 1000 0.6000 0.7000

.0.7000 0.1000 1500 0.0000 0.0000 0.0000 0.0000 Transform: Arcsin Square Root 1-Tailed Conc-mgIL Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD 0-Control 0.9000 1.0000 1.2543 1.1071 1.4120 9.935 4 250 0.8500 0.9444 1.1781 1.1071 1.2490 6.954 4 0.554 2.360 0.3246 500 0.8000 0.8889 1.1254 0.9912 1.4120 17.662 4 0.938 2.360 0.3246 750 0.7500 0.8333 1.0584. 0.8861 1.2490 14.733 4 1.425 2.360 0.3246

1000 0.5250 0.5833 0.7975 0.3218 0.9912 40.253 4

3.321 2.360 0.3246 1500 0.0000 0.0000 0.1 588 0.1588 0.1588 0.000 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.94207 0.868 -0.8093 1.72125 Bartlett's Test indicates equal variances (p = 0.25) 5.33512 13.2767 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 750 1000 866.025 0.26077 0.28874 0.12228 0.03782 0.04222

4. 15 Page 1 ToxCalc v5.0 ~~~~~~~~~~~~~~~

by: -1 i ToxCalc v5.0 Page I

Larval Fish Gi Start Date: 418103 13:00 Test ID: refO4O3fg End Date: 4/15/03 13:00 Lab ID: S&ME Sample Date: Protocol: EPAF 91-El Comments: Conc-mg/L 1 2 3 4 D-Control 0.3750 -0.4380 0.4910 0.4320 250 0.4520 0.3270 0.3100 0.2370 500 0.3030 OA720 0.3590 0.2480 750 OA730 0.2480 0.3570 0.3280 1000 0.3410 0.3220 0.3510 0.0220 1500 0.0000 0.0000 0.0000 0.0000

owth and Survival Test-7 Day Growth Sample ID

REF-Ref Toxicant Sample Type: KCL-Potassium chloride DA Freshwater Test Species: PP-Pimephales promelas Linear Interpolatlon (80 Resamples) Point mg/L SD 95% CL(Exp) Skew IC05' 59.5064 108.319 23.9033 806.72 2.8051 IC10-119.013 181.187 47.8067 1137.14 2.0208 IC15* 178.519 245.365 71.71 1172.32 1.1021 1.0 - IC20* 238.026 293.221 95.6134 1479.06 0.1239 IC25 801.69 301.38 0 1189.49 -0.3674 0.9 IC40 995.825 122.377 540.058 1218.91 -1.3704 0.8 IC50 1081.08 92.9263 770.357 1265.02 -0.7196

indicates IC estimate less than the lowest concentration 0.7
0.6

° 0.5 i 04 0.3 0.2 0.1 0.0 0 500 1000 Dose mg/L 1500 2000 Reviewed by: UD

1 W.

1;.. i -S ToxCalc v5.0 Page 1

- ~ ~ ~ ~ ~ ~ -}r Sr i Larval Fish Growth and Survival Test-7 Day Growth Start Date: 418103 13:00 Test ID: refO403fg Sample ID: REF-Ref Toxicant End Date: 4/15103 13:00 Lab ID: S&ME Sanple Type: KCL-Potassium chloride Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: PP-Pimephales promelas Comments Conc-mg/L 1 2 3 4 D-Control 0.3750 0.4380 0.4910 0.4320 250 0.4520 0.3270 0.3100 0.2370 500 0.3030 0.4720 0.3590 0.2480 750 0.4730 0.2480 0.3570 0.3280 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Moan Min Max CV% N t-Stat Critical MSD D-Control 0.4340 1.0000 0.4340 0.3750 0.4910 10.930 4 250 0.3315 0.7638 0.3315 0.2370 0.4520 26.944 4 1.730 2.290 0.1357 500 0.3455 0.7961 0.3455 0.2480 0.4720 27.710 4 1.494 2.290 0.1357 750 0.3515 0.8099 0.3515 0.2480 0.4730 26.514 4 1.393 2.290 0.1357 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9149 0.844 0.46543 -0.5616 Bartlett's Test indicates equal variances (p = 0.71) 1.39661 11.3449 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 750 >750 0.13567 0.3126 0.00859 0.00702 0.34353 3, 12 pjleSo-3.3 Reviewed by: k:.() ToxCalc v5.0 Page 1

Daiy Chemistry Data- - Client: S&ME Reference Test Location: S&ME Inc. Laboratory Template No.: -.-*. Fathead Minnow 7-day Chronic Analysts: Date~s:t s' r16.a11 ga113e M Ageoflarvae:

444s, -

h ^ Day Control: (Mod.Ha O 2 3 4 5 6 7 Remarks Temperature-Initial 26

a.

z SA Final D.O. Initial C 1A s o.? .o Final _l.O b.8 U.o L.&5 ls______I pH Initial n-7 m9 *-.9 O.0

t.

c Final _1._ U I 1 7. Alkalinity X 3.u - 59 5 6e Hardness qQs CiL9 t& Conductivity 3)3 aq 3741 399 3%i 38Mc,i Analyst Initials T ('0 l QA Review Initials S_p I r)1 _D a 250 mg/L (KCl) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 2.S F) s S(U i t Final _ 3 i '4 S 2.2 3S_________ D.O. Initial (¢.L ( _o_________ Final _.O O 5 c.2 5

3 pH Initial C° Al A Final

'195Lu. O1 Conductivity dt I~2"S ! ~_L" Analyst Initials P EjD P QA Review Initials / Prn OY "I Day 500 mg/L (KCI) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 26 S 3 c 24-j 56 Final S2I.S 25. QS.2. _______2S_. D.O. Initial -Y1. t6.( Final _5 l 0 6 -___ pH Initial -0 6 s \\ M Final I7 3 1. g Th(o Conductivity 1 a 1 1Zd \\v-A Io As Analyst Initials 4L P., a I 1_ QA Review Initials Ž o pt r,o t: I NOTES: Page 1 /A

Daily Chemistry Data - Fathead Minnow 7-day Chronic Client: S&MIE Reference Test Analysts: i Location: S&ME Inc. Laboratory Dates/times: '1IL/o, 3 i13._ - '5 Template No.: Age of larvae: 21 hs ,, e z l, Vr. . ' i '- 'r s ?,',. K..... K. s . -;} i I J 6 1 3/1 3

V;.,

Day_ 750 mg/L (KCI) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial -ZS. > 7 V&'i T4 as.v.Q _2 s_ Final 1 D.O. Initial GS_______ Final _-R .S 3 Su __5__ pH Initial

0_

___P.\\0 Final _ 1 4 S_________. Conductivity VSG? rI4E-9 6Z LLD I U%L4 Analyst Initials -r I X QA Review Initials h I I Da lOOOmgfL(KCl) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial QFB r Final 4

2. 12.

__ __s_ j_ D.O. Initial U.1-; 1LO,[ Io. U Final t.O s._ 4 pH Initial

8.

s). .c O t Final 1 m.S ____1 _ ____ Conductivity I 9 I

q a5200 Q LD' 5Qac'S c

___L Analyst Initials 4}O 4a S- -r QA Review Initials 0 S -1 r 1500 mg/L (KCl) 0 1 2 3 4 5 6 Remarks Temperature-Initial 25q.9 8 It Final / ________, D.O. Initial 4.$ I6 0(9 / _ Final L.o 1Q I / _ l pH Initial 8.0 D 0 1 I O_T-Final i I I Conductivity 92l q'D '9MI Analyst Htials 3 e A / I t QA ReviewlInitials ~~ 4 ~ __I J I Stock Solution Date qi A413 WS 8 - _13 - NOTES: Page 2 ,.,1 -.. 04 !s,. n.; -g s oR':': l St' o' 1' - z _.. i W.r, 5-jo -? 9. I

b-& ~~~~~~~~~~NO. 036 P. 2 SaMEwCD chronfc Control curt Up4swe April ,2003 IC25 NaL Toxtcty Tst Coitl Chat (CD) Somme PLL S&ME S&MS S&M S&MB SAM S&M SNmE S&ME SAME SNmE SAME ShNE sm a&E ShWE 11127/01 12/I 0/01 02/11/00 03/13/02 003/02 OSA/ 2 0011: 07/30/0: 03/1310: 09/1310: 10/21IM: I lI1BM, 12/16/0: 0234/. 0310310: 03/17/02 04tOt 0: T=25 mmha S iS MoAn-2s WO1S 1.1200 1.2900 31A200 1.0700 1.1700 1.0800 1.0500 0.5700 1.0800 1.0900 1.1400 1.1200 0.8900 1.2000 1.100 1.1000 0.9 too 0.9900 1.1006 1.050 1.1767 1.1775 1.1560 1.1583 1.1471 1.1350 1.1056 1.1030 1.1018 1.1050 1.1062 1.0907 1.09B0 t.1019 1.1023 1.0911 1.0855 1.0563 Q1202 0.0981 0.002 0.0144 0.0757 0.0752 0.0776 0.1144 0.1081 0.1026 00o98 00944 0.1075 0.1074 0.1040 0.1016 0.1084 0.1079 0.1051 0.2404 0.1963 0.1603 0.1689 0.1515 0,1504 0U1553 0.22S7 0.162 02053 0.1970 0.118a 0.2150 02148 0.2098 0.2031 0.216B 0.2157 0.2102 0.9646 0.9804 1.0172 0.9g71 1.0069 0.9967 0.9797 0.8768 0.5868 0.8965 0.9080 0.9174 0,1757 0.832 0.3921 0.893d 0.8743 0.5700 0.0766 1.4454 1.3730 1.3371 1J249 1.3098 12974 12903 13343 1.3192 1.3071 1t3020 1.2M9 1.3055 13125 1.3117 1.3049 1.3079 1.3015 1.2970 Pag I 865 970 2312 MAY-13-_303 07:25 P. 002 CVY. 10.0 1.3 6.8 7.3 65 6.1 6.t 10.3 9.8 9.3 8.9 8.5 9.9 9.8 9.3 9.2 9.9 9.9 9.7 S&ME-Codo Chronc Conirf Chsr- _. _......S-8"n ................... 0 oan+2s IA 1.3 CA I - -- N I -1 I -1 I -1 I I I I I Iff 0 tel tdEP Nsl o l,-'e 'l -Zlo e, 46 l c>tbo4 1'45 p T sn Dalc .. ebm ann - * - I U-1. Q. r-QUZ to @ I C: I 11 I

I'LJ. J*4 Sodium Chloride Chronic Reference Toiicant Data for Ceriodaphnla drbia asing Moderately Hard Synthetic Water Test date Control Mean Reproduction cv CT MSD PMSD 1 10-29-01 2 11-27-01 3 12-10-01 4 01-22-02 5 02-18-0: 6 03-13-0' 7 0403-02 8 05-15-02 9 06-11-0:' 10 07-30-0' 11 08-13-02 12 09-13-02 13 10-21-0' 14 11-18-02 15 12-16-0 2 16 01-08-03 17 02-24-03 18 03-03-C3 19 03-17-(3 20 04-08-( 3 (offspringlfemal) 27.7 23.2 24.8 23.5 17.3 23.3 24.6 28.6 28.7 20.6 29.7 28.8 25.4 29.3 24.9 20.8 26.8 31.4 29.0 24.9 for Control (%) Reproduction CV -(0/%) 10.1 8,6 12.3 8,1 9.9, 8.0 10.4 6.7 9.2 8.0 6.2 6.0 11.0 13.7 7.4 7.0 8.1 5.1 6.2 21.5 8.6 10.5 9.7 9.7 9.4 9.6 9.2 9.2 9.0 8.7 8.5 8.7 9,1 9.0 8.8 8.8 8.6 8.4 9,1 (%/6) for PMSD () 3.0 4.3 2.1 4.3 6.1 4.7 3.2 6.2 6.1 5.0 6.2 5.0 5.5 9.6 3.6 4.0 4.0 6.2 5.6 4.5 10.8 18.5 8.5 18.3 35.3 20.2 13.0 21.7 21.3 24.3 20.9 17.4 21.7 32.8 14.5 19.2 14.9 19.7 19.3 18.1 18.5 13.5 15.1 20.1 20.1 19.0 -19.3 19.6 20.1 20.2 19.9 20.1 21.0 20.6 20.5 20.1 20.1 20.1 20.0 MAY-13--03 07:26 865 970 2312 P. 004 Test number CT I

Sodium Cb!oride Chronic Reference Taxleant Conhrl Chart fnr C.et;tapltwtia dUlda usingModerately ard Synibelic Water Test Test ilate 7-d IC25 CT Number g/LNaC) (g)LNaCQ) S CoantrolLmits SA.s WarningLimits SA. CT -2S CT+ 2S CY - S,,. CT 4 S,L Control Ikuta CT-SA" CI+SAM 1 10-2901 1.120 2 11-27.01 1.290 3 12-10-01 1-120 4 01-22-02 1.10 5 02-18-2 1.070 6 03-13-02 1.170 7 04-03-02 1.080 8 05-15-02 1.050 9 06-11-02 0.870 10 07-30-02 1.080 11 08-13-02 1.090 12 09-13.02 1.140 13 10-21-02 1.120 14 11-18-02 0.890 15 12-16-02 1.0 16 01-08-03 1.160 17 02-24-03 1.100 18 03-03-03 0.910 19 03-17-03 0.990 20 04-08-03 1.107 1.205 0.12 0.96 1.45 0.54 1.177 0.10 0.98 137 0.53 1.178 0.08 1.02 134 0.53 1.156 0.08 0.99 132 0.52 1.158 0.08 1.01 131 0.52 1.147 0.03 1.00 130 052 1.135 0.08 0.98 1.29 0.51 1.106 0.11 0.88 1.33 050 1.103 0.11 0.89 1.32 0.50 1.102 0.10 0.90 1.31 0.50 1.105 0-10 0.91 1.30 0.50 1-106 0.09 0.92 1.29 0.50 1.091 0.11 0.83 1.31 0.49 1.098 0.11 0.88 1.31 0.49 1.102 0.10 0.89 131 0.50 1.102, 0.10 0.90 1.30 0.50 1.091 0.1 0.87 1.31 0.49 1.086 0.11 0.87 1.30 0.49 1.087 0.1 0.88 1.30 0.49 0.66 0.65 065 0.64 0.64 0.63 0.62 0.61 0.61 0.61 0.61 0.61 0.60 0.60 0.61 0.61 0.60 0.60 0.60 1.75 1.71 1.71 1.68 1.68 1.66 1.65 1.60 1.60 1.60 1.60 1.60 158 1.59 1.60 1.60 158 1.57 1.58 0.75 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.68 0.68 0.69 0.69 0.68 0.68 0.68 0.68 0.68 0.67 0.67 OA6 0.45 0.45 0.44 0.44 0.44 0A3 0A2 0.42 0.42 0.42 0.42 OA] 0.42 0.42 0.42 0.41 0.41 0.41 1.95 191 1.91 1.87 1.88 1.86 1.84 1.79 1.79 1.78 1.79 1.79 1.77 1.78 1.79 1.78 1.77 1.76 1.76 0.10 0.08 0.07 0.07 0.07 007 0.07 0.10 0.10 009 0.09 0.09 0.10 0.10 0.10 0.09 0.10 0.10 0.10 ANoia 7d IC25= 7-day 25°f ihibition concentratio A estimaion ofthe conccntrtion of sdium chlonideiiatwoild ause a 25%°reduction in Ceriodnpbn reproduction forthe test population. CT= Central tendency (mean IC25). S= Standard deviation of ihce1C5 values. S.S= SLandarddeviation codsodinyto the 75ipumentiIeCV. SAs=A0.45, as determined by USEPA forthe method and endpoint. S,LsO=Standarddeviation corcsponling tolbe 90m pertenbme CV. SA99- 0.62, as dtna bytheUSEPAfr (eiLod and adPoiaL CV C i n. it C)t V.liId(IU( tC T., t.ahu. IJSIPA. 2000. Understanding and Accountiag for Method Varablityin Whole Efflunt Toxicity Applien sUnderthNadonal PoLutant Discharge Elimination Profrau.EPA-8 33R-00-003-USEuvironmental Protection Agency, Cincinmti, OIH (A (JT 0 2c 3.J I0 U. 01 m LA rI-CV I. I t

~ Iv LN. 036 P. 5 CER1ODAPHNA TEST INFORMATION SHEET Tesr Name i nia Refeccel Test Cod4cted Pr=: Ib5 (-ay O) To .JJJ 1 3 (Day7n Sites/concentratiov: I1. rx 4

2.

.,iDnIL S. \\!r 3 3D L,

6.

o 9 Stack (if aplicabl):- Control Water Tip. ('): 20% Dii.te Mineral War + Trace Minerals Other (dcscriba): 2 Dilution Water ype ('): 20% Dil ft Mineral Water + Trace Minerals Otber (d scribe): _._:_Q Source o Test C rganisns: S&MB braod board nos. Age or Test Org vaisms: IsolatedneonateE forteston d11Ila (date)rtom MI5 to i1SS... (time) klitalsJ Record of rvin Test Non-Conformity-Date: Descrf rion of Non-.Conforeity: Initias Date: Descrbtiaon of Non-Conforaity: Test Log sliceis cA Reviewed (Ravi ver Initials) on (DMa) ,ol, rhuuy 0 (caicd:zI sh ) c303 07:26 865 70 23i2 P.005 MAY--

Ctxiodaphnia Daify Test infrmatiunLogstheet hestNrne: SMEChmniRefecrnceTest TestDates: Al f,% -~""~ .~ ^ ~ I iest Iaton, water iaformaticn (cp idC) ______~ ~ ~ I___ Tton CcmtrlWater Carboy No, and Ailom1 commnenis Date and Inabator Temp. (C) Neo. Fed 100 YCIDa Fed 100l Date stTM= End iiL melanasfn:l am.tfids 'NIO. YTDlSeienastn Date _aim___ C___ Q oy# D afeIX p Day O 0 %, O-, A.D t ID,5 2a:sldb~ I S

}Il Sl5 l9>

Day Day 231~ ,1 olo! 15 C 44S~ :?sb Li4 Lf 1lZ tw Lt4!5 3 1% Day 3 %@, S 2S. l35O 4s103 )3& 3/0o3 U3-3c 13SQ 3 t//O3 DayS 4 S .o )aSlu3 1 apS(13 to 3 hl Day 5, S. CyS 5113 45 3t13 13 S-<C5 I 1no 4110fos ( ,eac) 2J161,3 3/7i/b3.131S 1:330 Isqo 034IPe 'C (4 r!) m m I3~ (4 N? Wo Ni F -a -,S -- _ Dql1b 7 S6 AS 1

Client: Chronic.eferc-i Location: S c Analysts:.an, Ise e5: __ 41;E Ceriodaphnia 3-Brood Survival ancl ReproductionI Raw Data Sheet ce Tst A I Repicate lio. of No. of live No. of Yol?Per l l1 2 3 l4 51 6 718 l9 10 Young Adults Oiginal Adults Adult Conc. aIrit. Day Control 3 4 t a._. to 5 I% °. °O JA. 6 a c: cf o Q Q E A o I o 7 _oc m %01 v o k' %L C95 CI o o 112 q o Replicate No. of No. of live No. of Young Per 1 2 3 4 5 6 7 8 9 10 Young Adults OriginalAdults Adult uit Day-oo 1 O 500 3 C J.f mg/L 4 5 5s L3 t t° NaC1j u 5 80 10 -1 k2 % 6 o Q° 14 I 7 7 s to %LNV -0 1d in Tu-1 I I -oal -S-S

s....

4 1 hi h.o 1o 750 mg/L NaCl r . Replicate No. otiNo. of live No. of Young Per I t.) to Total-133la( t I38 A342, ll.; I k 1 .4 1 5. .4.. b .4 5 1 S .4 1 S. S hi .ia S I 9.?- Page 1 MAY-13-2-1303 07:27 865 970 2312 P.009 No. of Young P r Onrial Adhs Adult Init Day i L _0 tOOI___ L1 sc.lltloatztMsi9e I.O 0 e 1. t1 7 2 13' 1c)t1 2L' 1tc tn Im °1 E--6 LL Q L .i ~~~~~~ ~~~~~~~~1 - -11. JS s103 Replicate No. of No. of live1 _l 1 2 1 3-1 4 1 61 7 -s 1-9f I 0 Youngl Adultsl

NO. 036 P. 10 Ceriodaphnia 3-Brood Survival and Reproduction Raw DBta Sheet Client: Chropiq_efte,3-eLIat Location: S&ME-Jrc. Analvsts: 4Pio. S4 -h.b.. . - I .y - I - 1000 mg/L NaCi ~~~~~..- _4 A .6 Replicate No. of No. of livel No. of Young Per 1 I 1 2 3 4 5 6 7 8 9 10 Young Aoults OriginalAdults Adt rnit.lDay--- -l 54& 12o 1 1).-- 1 1 4°'e1x 15OO 4 1.. a O 4 mgJL 5 NaCI 6t 6 z 7 to Me.!: oovive o 2 3 6 -Ad1,, Init. Day I~~~I~ ~ ~~~~~-i I_ C 2000 H mg/L 5 NaCI 6 7 1it Page 2 MAY-13-2;003 07:27 865 970 2312 NR t o, of No. vflive No. of YoM Per younI Adulk I du Adult Init. Day - 6 4 14 I M Ii q a K S 3-AP A.- J ~ 7 Q O> 000 0O ~ 2~ 3,o So 3p ts~.. P. 010 1, eficate I 2 3 6 7 8 9 10 PY) Total X;IaS I 1\\ ~ At I zi al _- 1 ab I i7 1

T036 P.11 1 Carloda hation Test-? Survival Start Data: 45G/2003 13:45 eat iD: Re40 Sample ID; REF-Toxicant Enq Date; 415/2003 1330 Lab ID: S&ME INC. Sample Type: NACL-Sodlum chloride Sample Oate: Protocol: EPAF 81-PA Freshater Test Specles: CD.Cerlodaphnla dLubia Comments: Cono'inpIL 1 2 3 4 5 5 7 10 D-Control 1.0000 1.000 1,0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 600 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 760 1.0000 Locoo 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1000 10,000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1500 1.0000 1.OC00 0.0000 1.0000 1.0000 1.0000 1.0000 0.0000 0.0000 1.0000 2000 0.0000 0.000 0.0000 0,0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 Not Fishera 1Tailed Cono-mg IL Mean N-MEan Rasp Resp Total N Exact P CrItical 0-Control 1.0000 1.0.O0 0 10 10 10 So 1.0000 1.OJO0 0 10 10 10 1.0000 0.0500 750 1.0000 1.0a00 0 10 10 10 1.0000 0.0500 1000 1.0000 1.0)00 0 10 10 10 1.0000 0.0500 15Cc 0.7000 0.7300 3 7 10 10 0.1053 0.0500 2000 0.0000 0.00 10 0 10 10 Hypothusis Test (1-tall. 0.00 NOEC LOEC ChV TU Flsher's Exact Test 1500 2000 1732.05 Page 1 ToxCalc vS.0 Reviewed by: Wo IY-13-2:.03 07:27 865 970 2312 P.011 w -r I I

JWE J.;l-1 NO. 036 P. 13 Start Date: 4/8/2003 13:45 End Date: 4/15/2003 13:30 SaMple Date: Comments: Conc.rmg/L 1 2 D-Control 37.000 23.C56 500 28.000 2B.C30 750 27.000 33.COO 1000 22.000 1B.COO 1500 9.000 D.COO 2000 0.000 O.-C00 Ceriodaphnia Survival and Reproduction TestRaprodUction Test I: Ref4O3ar sample ID: RE-Ref Toxicqnt LaJ ID: S&M9 INC. Sample Type: NACL-Sodhum ch10ilde ProtocoI: EPAF 91-PA Freshwater Test Spedes: CO-Cerlodaphnlis dubla 3 4 5 B 7 B 0 25.000 28.00 26,000 54.000 28.000 18,000 24.000 18.000 21,000 23.000 21.000 26.000 20.000 22.000 31.000 23.000 28.000 28.000 28.000 2a.000 31.000 34.000 28.000 26.000 25.000 30.000 23.000 21.000 27.000 24.000 20.000 17.000 0.000 8.000 13.000 14.000 17,000 2.000 0.000 12.000 0.000 0.000 0.000 c.ooo 0.000 0.000 0.000 0.000 Transforr: Untransformed 1-TalIed isotonic Conc-m IL MOan N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 24.90 1.0300 24.900 18.000 37,000 21540 10 26.200 1.0000 600 25.000 1,0J40 25.000 20,000 31.000 14.727 10 -0.049 2.223 4.509 26.200 1.0000 760 28.700 1.1526 28.700 26.000 34.000 10.264 10 -1.874 2.223 4.509 2.200 1.0000 1000 22.700 0.9116 22.700 17.000 30.000 17.027 10 1.085 2.223 4.509 22.700 0.8564

150C 8.400 0.3373 8.400 0.000 17.000 71.252 10 8.136 2,223 4.509 8.400 0,3206 200C 0.000 04C)00 0.000 0.000 0.000 0.000 10 0.000 0.0000 AuxliaryTests Statistic Critical Skew Kurt Shapiro-Wilk's Test nqIcates lornal lstrbu1on (p >0.01) 0.98276 0.93 C,27392 0.28416 Bartlett's Tes: ndlcates equa' vadances (p 0.24) 5.54255 13.2767 y

t NOEC LOEC ChV TU M3SPu MSDp MSB MSE F-Prob df Ounnett'sTest 1000 I=00 1224.74 4,50922 0.18109 B19.33 20.5667 3.3E-12 4,45 Llnear Interpolation (80 Resamplos) mglL £O 95% CL Bow 843.571 92.6739 775.866 1010.87 -2,5223 937.143 64.3879 837.834 1048.29 0.2867 1015.03 55.1818 899.347 1085.65 -0.1835 1.0 1060.84 48,3488 949.14 1126.69 -0.2786 o.9 1106.54 45.1469 999.048 1173,16 -0.2564 0.8 1244.06 39.1017 1166.64 1320,39 0.1802 0,7 1335.66 42.3373 1253.65 1431.73 0.2024 n R .6 CD 0.6 " 0.4 0, x 0.2 0.1 0.0 -0.1 -0.2 0 500 1O0 1500 2000 2500 Dose mgL ToxCalo v5.0 Revlewed byjl. MAY-13-Z.O3 07:28 865 970 2312 P. 013 Polnt iC05 1010 I1S IC20 IC25 IC40 1C0O Page I I

-xI Wc Cariodaphnla Survival and RerroductIon TestaReProducton Start Date: 4182003 13:45 Test ID: WOW sample ID: REF-RefToxicant End Date: 4/12003 13,30 Lab ID: SME INC. Srmple Type: NACL-Sdlum chlorlde Sample Date: Protocol: EPAF 91 -EPA Freshwater Test Speces: CD-Cerlodaphnin dubJ; Comments: Conc-mg/L 1 2 3 4 a 6 t 10 D-Control 37.000 23.C30 25.000 25.000 28.000 24.000 28.000 18.000 24.000 1.000 600 28.000 28,COD 21.000 23.000 28.000 26.000 20.000 22.000 31.000 23.000 750 27.000 33.C 00 2B.000 28.000 28.000 26.00D 31.000 34.000 28.000 26000 1000 22.000 18.C00 25.000 90.000 23.000 21.000 27,000 24,000 20.000 17.000 1500 9.000 9.( 00 0.000 8.000 13.000 14.000 17.000 2.000 0.000 12.000 Trf¶fom:Untransfod -aie Conc.nm IL Meain N-MLan Mean Min max CV% N t-Stit Critcal MBD D-Control 24.900 1.0000 24.900 10,000 37.000 21.540 10 B00 25.000 1.0340 25.000 20.000 31,000 14.727 1D -0.049 2.223 4.509 750 28.700 1.1 26 2B.700 2B.000 34.000 10.264 10 -1.874 2.223 4.509 1000 22.700 0.9115 22.700 17.000 30.000 17.827 10 1.085 2.223 4.509 '1500 8.400 0.2373 8.400 0.000 17.000 71252 10 8.136 2.223 4.509 Auxiliary Tests _Stati c Critical Skew Kurt Shmpiro-Wil's Test Indicates ormel distrlbution (p> 0.01) 0.98276 0.93 0.27392 0.26415 Bartlets Tesi indcates equa. varlances (p = 024) 5.51255 13.2767 Hypothesis Test (1-tall.0 NOEC LOEC ChV TU MSDu MSDP MS MSE F.Prob df Ounnets Test 1000 1500 1224.74 4.50922 0.18109 619.33 20.667 .3E-12 4.45 Page I ToxCalc v5.0 REF lewed by: iMY-3-:ge03 0:28 eS 970 23i2 P. 8i2 Sb ~P. 12 1

NO. 036 P. 7 Dall; Chendtry Data - Ceriolaphula 7-4ay Chronc Client: Refe¢ne l tet Analysts; 31Afi. Location: S

Inc, DatWstimcs:

TemplateNo.: j Age of Neonates: haa Control: (20% DMW) 0 1 2 3 4 5 6 Rema rks Final 4.S 2S 2A-I t'jv.L a D.O. Initial IL, t_t_ -______ Fital _ G. S 5 .0 pH Initial f3,.C -4.q 18. d%S kd,O FinalA1_ u ^ 4 Hadness qo 8 Conductivity rn P6 hPL %u Analst Intials jen 'h j j~- 4j - V-y I A- - I -a 1 2 3 4 Remaks Temperature-Ini as. I 1 5 D.O. initial 0 Final I t@. ut. pH Initial f3%.c oc9\\. Final v -.O (Jon~~~ ~ ~ ~ ctutit A _ IA1L I ~S Y._ AIkA x Analyst Initials QA ReviewlIniti is -1 ~,-M !&-1 i.-1~.2 750 mg/L 0 1 2 3 4 5 6 7 Remarks Temperamre-iial .2 I ar.t Pill1 i 6 la ls Final _t L S -Le s.9 pH Initial %- a .o %" fiinal a 1.0 I -t -. a ,[ Conductivity !13 LXtSi. 1LJ_-ls LX _ nalyst nitials P10 m 4 J Y 3 L_ QA Review itials -cz Al. =0 O P-Ito el" 6A x., I Al. Page 1 MRY-13-:.303 07:26 865 970 2312 P. 007 t, 6k*tJ: L "S Q* Ic as, e r _r r _5 Day AikalimtV e' A

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T - L!+ I - I - I Conductivity 106tItNtlHIMA lV as" I WE tS I ";-~VL .,t ~a s " L u

MO.036 P.S ~~~~~~~~~~~NO. 03 P. e Daily Chemistry Data - Cerlodaphnia 7-day Chronic Client: t Analysts: Location: SWl lm Dates/times: Template No.: I Age ofNeonates: .e-. 3%A.Co 1000 m 1 2 3 4 5 6 7 l marks Teraperatime-Initi-, 21 0411 Os;___ ha25121 Final__ .29[

2 o

s.1 D.0. Inltial

6. S 9

U." U b5 Lt. Final. .'- 6 t G(i. -r t..S 15a pH Inii 8'. I. IR. .0 %.O t-I -N) Final I -..,A Conductinity Je qf ;oo NO4 W_ Analyst Initials w X Sj QA Review Initia s T4 gw iF o 1500 mg/L 0 1 2 a3 4 5 6 Remarks Temperature-Init al =S Tq l.9 -;l 2S. q Fin [ N.1 ewo ZI*o 2 D.0. Initial L.._ 1s 6.i h LS U Final -Final j I Conductivity _=0 2Kla 2 =110 RE t_ Arialyst Initials 1L A 1l QA Revie tils Ag g 1M ft-. Ž. __I ~ ~ ~ ~.Day_ 2000 mgL 1 2 3D V 5 6 Rem Temperature-hi-.ial I-5 q 1 qv !AA. )4g / Fill 1/ D.O. Initial L. 5 Final pI-I Initial .o _____LI I-Final Coinductivity D L n AnalystIitials>b _& Stock Solution Date ___t= Page 2 MAY-13-: 003 07:27 865 970 2312 P. 008 Day I I CqP}}

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