ML030660611
| ML030660611 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 02/11/2003 |
| From: | Beavers M Tennessee Valley Authority |
| To: | Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML030660611 (102) | |
Text
-J Tennessee Valley Authonty, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 February 11, 2003 State of Tennessee Department of Environment and Conservation Division of Water Pollutilon Controf Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah
Dear Mr. Hannah:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Please find enclosed the Discharge Monitoring Report (DMR) of January 2003 for Sequoyah. Please contact me at (423) 843-7324 if you have any questions or comments.
Sincerely, Michael G. Beavers Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013
- U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pnmted cn cyded pap:,
PERMITTEE NAME/ADDRESS (Include FacifiItNamelLocationifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 Address P 0 BOX 2000 (SUBR 01)
.{INTEROFFE SB-2A--) TN0 450 ]0G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE Facilyt. TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT Frm10 IFYEARmI MO1 DAY To MO D NO DISCHARGE I- ..
ATTN. Michael G. Beavers o To10 01 3 NOTE Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 9.2 04 0 31/31 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 .. PERMrT-,-;**
INSTREAM MONITORING 'RIEQUIREMENT: ~~')2~
~:~; 'u~~AjQ~
~05-.DGC
~ -DAILY Mx" ____
ý EES
- 2'RMT RE TEMPERATURE, WATER DEG. SAMPLE ** 23.2 04 0 31/31 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 0 1 PERMIT ' ;***, '** REOT DEG. C. ~ KSEEf ~CK REQ; EFFLUENT GROSS VALUE . EME,
.. . ,: , - *-, , .. ,-> ... - IL.o X-.
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PH SAMPLE 7.6 8.0 12 0 10/ 31 GRAB MEASUREMENT 00400 1 0 0 'VIIPERMIT ;* ,*,**,***.T : ,**.**- , '. , - SU ... WE . G...
EFFLUENT GROSS VALUEREQUIREMENTi', .......... ..........
-MINIMUM' -*-';-....'
i< MAXIMUM ____ ~'
SOLIDS, TOTAL SUSPENDED SAMPLE 4 5 19 0 5/31 GRAB MEASUREMENT 00530 1 0 0 ."K PERMIT; , . .3, *, --.-
7 ; 0T -RAB
- M WEEKLY, EFFLUENT GROSS VALUE ,.r,4
- iYc OAG ýDAILYMk- ____ ~2iu~
OIL AND GREASE SAMPLE **<5 <5 19 0 5/31 GRAB MEASUREMENT 00556 1 0 0 P~ERMIT.'--~ *~**~ '2 GLWEL'~GA EFFLUENT GROSS VALUE IN K"P '"MOA G' -DAIILYX 44 ___ ___
FLOW, IN CONDUIT OR THRU SAMPLE 1614 03 ***0 31/31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 0 PERMIT - .**,k,** *>REPORT MGD ". * '- ' ,** * * . o*' CONTIN RCORDR EFFLUENT GROSS VALUE .. DREQUYREMEN -".... .. ..
CHLORINE, TOTAL RESIDUAL SAMPLE ** <0.005 0.016 19 0 38/131 GRAB MEASUREMENT 50060 1 0 0 -ý;,,PERMITi* . *-- * - . .... 7 7"; -7..
50060 1 0 0-*;*******'-*,* - ....-*** .... . ,,** " * n -* MG/L :,,*i WEEK-> CALCTD IREMENT, f-..... DREQ "
EFFLUENT GROSS VALUE -~, 'r~~~r' -,INS MX' J____ ~~'AS NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel *,
Richard T. Purcell property gather and evaluate the information submitted Based on my Inquiry of the person or J persons who manage the system, or those persona directly responsible for gathering the Environmental Supervisor Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, EnvironmentalSupervisr423 843-6700 03 02 13 accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following Injections occured 1. PCL-222 (max. calc. conc. was 0 026mg/L--limit 0 100mg/L) 2. CL-363 (max. calc. conc. was 0 009mg/L-lhmit 0 100mg/L) 3. PCL 222/PCL-401 (max. calc conc. was 0 020mg/L--limit 0.100mg/L)
EPAo Form 3320u-l* (REV 3199) Previous editionsma be usedl Page 1 of 2
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01)
Address P 0 BOX 2000
&INT-ERO-FFIC1E S B3-2A---.) -- TN0026450 !101 G F-FINAL SODDY-DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER DIFFUSER DISCHARGE FalitY A._A-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT SR MO DAY DAMOAY NO DISCHARGE = ...
ATTN. Michael G. Beavers From 301 01 To 01 31J NOTE: Read Instructions before completlnq this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE MAXIMUM TEMPERATURE - C, RATE OF SAMPLE 0.2 62 0 31 /31 CALCTD CHANGE MEASUREMENT 82234 1 0 0 ; ,PERMIT', ,******,, .' ,***** . *. ' ,0N.IN. 2.0..
.CALCTD, DEG C EFFLUENT GROSS VALUE ~$:> ~ ~ '~-'. .. r " -- ,tiEET~~' ' ,
TEMP. DIFF. BETWEEN SAMP. & SAMPLE ** 2.4 04 0 31131 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 W 0 *<iPERMIT.-j* $ *** e**,;,*,* - ,,, , ,, 0'CALCTD DEG. C.
EFFLUENT GROSS VALUEMEAQUIREMENT SAM1, G SAMPLE MEASUREMENT SAMPLE MEASUREMENT
ý'0-5PERMIT&~ ' ~"' ,,,4,-*,jri~3/4 MEASUREMENT BORON, TOTAL SAMPLE NT
",REQUIR*EME .o,.; ... ' <02 .* <02 .... <02 19 0 1131 GRAB MEASUREMENT 01022 1 0 0 7- PERMIT GRAB EFFLUENT GROSS VALUE ~ E~RMN~~RPR EOT 'RPR~ GL :-'OC/ GA SAMPLE MEASUREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my I TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel .
Richard T. Purcell property gather and evaluate the Information submitted Based on my inquiry of the person or" persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor Site Vice President Information, accurate, andthecomplete information I amsubmitted Is,there aware that to thearebest of my knowledge significant penalties and belief, true,false for submitting 423 843-6700 03 02 13 SIGNATURE OF PRINCIPAL EXECUTIVE Tnformation, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
CCW data for Jan 2003 is attached EP.. om.. ....... 1....evou..i.io..m y.e.se.P.e 2 f EPA Form 3320-1 (REV 3199) Previous editions may be used Page 2 of 2
CCW Data forJanuary 2003 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mg/L)
January 23, 2003 < 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mg/L)
January 23, 2003 2.3 Page 1
PERMITTEE NAME/ADDRESS (Include FaciltvNamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No 2040-0004 Name TVA - SEQUOYAH NUCLEAR PLANT Address P 0 BOX 2000
-- ER-FOFFICE SB-2A) I TN0026450 1J 101T F-FINAL SODDY- DAISY TN 37384 PERMIT NUMBER IDSCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Faciitv TVA
" - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MfNITfr)INr. PF:pIrlf I EFFLUENT YEAR MO DAY YEAR MO I DAY NO DISCHARGE [
- ATTN. Michael G Beavers From 03[ 01 01i To 103 01 31N NOTE. Read Instructions before comoletinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE IC25 STATRE 7DAY CHR SAMPLE >100 23 0 1190 COMPOS CERIODAPHNIA MEASUREMENT TRP3B 1 0 0 AERMIT. *;* " *,* " PERCENT ,- - QUART-r COMPOS EFFLUENT GROSS VALUE .. .. 4MINIMU tL -' ERLY _'_
IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT
- >100 223 0 1/90 COMPOS PIMEPHALES TRP6C 1 0 0 RE"-
T7,7PERMIr,-TT ' *6QUIREMENT; ***.4,,;
?*;- "...... ,. .....
- *, -,a"....°' .- ' . .
- 43i,9a ******** . PERCENT . -', QUART-; COMPOS EFFLUENT GROSS VALUE ~ -~~~' ~ Y '0r__ ________
SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE PERMIT *ai,~~' .
REOUIREMENTf--
MEA SU REME NT '~ ;A 1 ~
SAMPLE MEASUREMENT PREMIT a j, A ". : r; "...... ,. !
SAMPLE MEASUREMENT PERMIT,-.K', . ~ :~i "A.~4t REQUIREMEN oae wth sassua t i personne / #*
a° -'
SAMPLE MEASUREMENT 4 ERMIT iREQUIRWNENT 1 ia-~ ~,aa~' r~P' ~-~ 7=72, NAMErTITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TEEHN ATE direction or supervision in accordance with a system designed to assure thaI qualified personnelPHONED or of the person Richard T. Purcell properly gather and evaluate the Information submitted Based on my inquiry persons who manage the system, or those persons directly responsible for gathenng the Environmental Supervisor information, the Information submitted Is, to the best of my knowledge and belief, true, 423 843-6700 03 02 13 Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled from Jan 12, 2003 to Jan 17, 2003. The report Is attached.
EPA Form 3320-1 (REV 3/99) Previouseditions maybe used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facilty Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No 2040-0004 Address P 0 BOX 2000
.{.INTEROFFIEB-2A.)
S TN0026450 1 103 G F -FINAL SODDY - DAISY TN 37384 PERMIT NUMBER I DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Fachity. TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MOQNITORING PERIOQD EFFLUENT YEARI MO DAY YEARI M NO DISCHARGE L . ..
ATTN Michael G. Beavers From 03 01 01 03T 01 131 NOTE Read Instructions before completinq this form f
I I
I; NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Envirbnmental Supervisor information, the Information submitted is, to the best of my knowledge and belief, true.
accurate, and complete I am aware that there are significant penalties for submitting false
____nair_4_____ 423 843-6700 03 02 13 SIGNATURE OF PRINCIPAL EXECUTIVE information, including the possibility of fine and impnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EP For... 0.. RE.....Pevou.ei.on.my.e.se.Pge1..
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of I
PERMITTEE NAMEIADDRESS (Include FacilityNamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Address P 0 BOX 2000 (SUBR 01) 0MB No 2040-0004 (INTEROFFICE S B-2A).) . . . . .
TN0026450 107G F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER METAL CLEANING WASTE POND Facili, TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT MO IDDAY YYEARI Y M DAY .. N DSHI ATTN Michael G. Beavers From 103 1 01 01 To1 0 1 01 131 "NO DISCHARGE Fxj NOTE' Read iflstructions before comoletina this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE MAXIMUM UNITS PH SAMPLE
- MEASUREMENT 12 30400 10 *1 *.PERMIT,-. I ****"* " -- ',,'*'**"i ** -; :- , *, ,;, '.*. ****. , : . *'; .... SU f*DALYRA 00400 1 0 0 4-1 EFFLUENT GROSS VALUE MtNIMUM'-../:'r'"
' !',,,,' AIMM _____
PHOSPHORUS, TOTAL (AS P) SAMPLE
- MEASUREMENT 19 00665 1 0 0 - PERMIT ,. **COM**P ,**.
~REQIREENT.-,.:~ .: ~ ~ , ~ A "-~ MGIL DAIL COIOS EFFLUENT GROSS VALUE -Y MX SOLIDS, TOTAL SUSPENDED SAMPLE **
MEASUREMENT 19 00530 1 0 0 -PERM-T-. , ',.-***. ****. "**-*, MG****'-,nAILML
, CDMPC S EFFLUENT GROSS VALUE , , AKA:. -DALM ____ ____
OIL AND GREASE SAMPLE MEASUREMENT ** 19 00556 1 0 0 -. ' PERMIT ' ,******B*;,2 .*** **,"***** , " " MGIL MG'2 'DAILY <GRB G-RA***,**,
EFFLUENT GROSS VALUE '-r'
.z .' DAILYMX'_ __
COPPER, TOTAL (AS CU) SAMPLE * **19 MEASUREMENT 19 01042 1 0 0 ",PERMIT '1 ***,*****,,',,,,*,****** P' * ""*** "' - **i*** . 0',,' MGIL ... DAILY,, COMP.OS EFFLUENT GROSS VALUE REURMNtn" '" k"~~ '., ~~,"
IRON, TOTAL (AS FE) SAMPLE 19 MEASUREMENT
- 19 01045 1 0 0 '*'* PERMIT - * . " ,....**,ij.,'
- *' 1**':
- "*-* ' ',1 MGIL/ :*'t DAILY.AICOMPcS EFFLUENT GROSS VALUE REURMN. .. ~".- >X7;~~" . ;*,,Ii', ;y.
FLOW, IN CONDUIT OR THRU SAMPLE **
TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 7...,PERMIT,'*3 11REPRT,, ,RO , "* '* -* .. ..... A CA.CTD EFFLUENT GROSS VALUE ,- "
""EUIEENAREPIORTr:
.. '.'* MOAVGV'ADA-L'Y...
MGD DAIY, CA NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and allattachments were prepared under my TELEPHONE DATE direction or supervision Inaccordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the Information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor SIte Vice President information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 03 02 13 Paccurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No phosphate bearing chemicals were employed. No Discharge this Period EPI-A F-orm 3320-1 (REPV 3/99) Previous editions may be used Page I of I
PERMITrEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Address P 0 BOX 2000 (SUBR 01) OMB No 2040-0004
- .-(.INTEROFFICE S B-2A . . . . . . t TN0026450 110 G -FINAL SOODY - DAISY TN 37384 PERMIT NUMBER DISCHARG NUMBERN RECYCLED COOLING WATER Facjh. TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIQR EFFLUENT ATTN Michael G. Beavers From1 IYEAR MO 01 DAY 01 To Ya o MOIDAY 01 131 .. NO DISCHARGE Fa] ..
NOTE. Read Instructions before cornotetina this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT ** 12 00400 1 0 0 PEMT r**& " > 'v.f> U EFFLUENT GROSS VALUE ,RQIEET P Lýýý i, -. - -, . . 6 , '., ,u1.- .ALY GRXIAB SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT 19 00530 1 0 0 PERMIT-', >27;***** ' DAILY, COMPS EFFLUENT GROSS VALUE ,RQIEET p 3I 'MGL .
OIL AND GREASE SAMPLE MEASUREMENT 19 00556 1 0 0 PERMIT-, ' *:{> ******* ******,,.- * , ,*, ;i*****,* ': 15.
. , M.IL ,; DAILY,. COMPOS EFFLUENT GROSS VALU :7' -' DAILY-Mx' COPPER, TOTAL (AS CU) SAMPLE 19 MEASUREMENT 19 01042 1 0 0 , *** , ! **,MGIL* ,*DA.* COMPS MPOSPERMIT, EFFLUENT GROSS VALUE * *************
ýYbAiLy, Mk,_____
IOTTL(SF)MEASUREMENT 19 01045 1 0 0 ":*;PERMIT:; *'*
...i* ****'
. .... *r.
. ;'***~***'*',...
... *;' ;:'-*- *~*******!;.= **** ; ' i*: 1- .... MG/L ;* DAILY,m -coMPOs EFFLUENT GROSS VALUE .:R..,.U....M:' *ti '.#'2
- MO>...*, ,..
- .. i, , ,.*, *r * . . ... - , *... ......
- PHOSPHORUS, TOTAL (AS P) SAMPLE MEASUREMENT 19 00665 1 0 0 '; ***" . 4**- ** . . 1 '" . MG/L DAlLY.
DPERMITA"COMPOS EFFLUENT GROSS VALUE REQUIREMENT. '2 -.... .. ' '.t 'A t -. "' ' ... .. -'. . ,X . ., ..
FLOW, IN CONDUIT OR THRU SAMPLE **
TREATMENT PLANT MEASUREMENT 03 EFFLUENT GROSS VALUE ER IT ;' TEURMEL P- EP O
-,~-
RT MGD* * ' "******* -, * *** , DA ILY
-PERM ' CA LCTD 50050 1 0 0 RfU ., .,
'AL NUMER EA MO DA NAME/TITLE TYPEINCODUI PRINCIPALORPHRINE EXECUTIVE OFFICER I Certify under penalty oifJawthat this document and all attach~ments were prepared under my cAMPETELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel /r,* V*.
Richa rd T. Purcell properly gather end evaluate the information submitted Based on my inquiry of the person or p:ersons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor42 83670 3 02 1 5005 1-r 0~u- 0XL SeVcrsdn nformation, the Information submitted Is , to the best of my knowledge end belief, true, f'rev 4r;`, PERMITions mayVý-becomplete se 2.4-70 Pge10 2 1 Sit VcePreidntaccurate, end I em aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYE*RPRNE nformation, Including the possibility of fine and imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUBE YARMO DA COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference af3attachments here)
No Discharge this Period EPIA F-orm 3320z-1 (RE::V 3/99) Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facihty Name/Location ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No 2040-0004 Address P 0 BOX 2000
- - .(NTEROFFICE SB-2.Aj. . . . . . . . . . I TN0026450 G F-116
-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER BACKWASH Failityv TVA. SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD i EFFLUENT 03 D
DY T11YEARMMO DY .. NO DISCHARGE FI1 ..
ATTN Michael G. Beavers From 01 01 To 03 01 31 NOTE" Read Instructions before comoletino this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1/ 31 VISUAL MEASUREMENT 0 *A 01345 1 0 0 PERMIT', ;.iE**,*',' *** ' * ". ,. ;*-**RPo , P .A.. =?S,,' ,&'2Ei'VISUAL,
'ýk*
EFFLUENT GROSS VALUE 'RUREE'T - ; " FAIL=i :PERMIT OIL AND GREASE VISUAL 'REQUIREM*;;-
SAMPLE :.; ",SR P0 R ,, Y S I.... ,REP.....ORV..
0 1/31 E~ *. "IU VISUAL L
MEASUREMENT 84066 1 0 0 PERMI -- I ;~IE~ EOT ** ** I~aaI ". 'VSUL SGRESUIREMENTV MEASUREMENT
~REQUIREMENT. ~
EFFUENUIREMENTE ' , " "-,~i.~-: "- *N= '*-v SAMPLE SAMPLE MEASUREMENT
,REQuIREMENTrý j > -' . , ~ ~ " . . , ,4' SAMPLE SAMPLE MEASUREMENT MEASUREMENT
"-PERMIT;V -~" i ~ 4 ~ N " , 7 4r4 4 ~ 4"
'REQUIREMENT >-' ~~ ~7: ~ *"a eaUin may oe se k-Eviu Pae 1of NAME/TI TLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my /* TELEPHONE DATE directhon or supervision In accordance with a system designed to essure that qualified personnel '
Richard T Purcell properly gather and evaluate the information submitted Based on my Inquiry of the person or I ,*J persona who manage the system, or those persona directly responsible for galhenng the Environmental Supervisor42 83-70 3 02 1 Site Vice President information, the Information submitted is, to the best of my knowledge and belief, true, 423____843-6700_____03___02__13_
accurate, end complete I am aware that there are significant penalties for submittinlg false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED information, Including the possibility of fine end ,mpnsonment for knowing violationsOFIEORATRIDAGN AEA COAEA UMR YAR O DY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes.
~r-r rormr ,33LU.1 II¶-V 3199) P'revious editions may be used/ Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilitVNamelLocation ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01)
Address P 0 BOX 2000
.. INTEROFFICE SB.2A). TN0026450 i 117 G F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER BACKWASH Faohi, TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY IMONITORING PERIOD EFFLUENT NO DISCHARGE L... .
ATTN" Michael G. Beavers From 3 01 0 To 01 31 NOTE" Read Instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE MAXIMUM DEBRIS, FLOATING (SEVERITY) SAMPLE 0** 9A 0 1131 VISUAL MEASUREMENT 01345 1 0 0 7. RMT-..., .. REPORT:$ PASS=0 . ,SEE' . VISUAL, EFFLUENT GROSS_ VAUEOTAL"
~REQUIREMElý~M FAIL=1
___ _-PERMIT yo__
OIL AND GREASE VISUAL SAMPLE 0 94 ** 0 1/ 31 VISUAL MEASUREMENT 84066 1 0 0 - PERMiT..2;r 'RPRT1 1 VEmI3/4 ***** -SE U EFFLUENT GROSS VALUE ,REO-IR.MENT , ,MNO=0 . ' * *.. >
SAMPLE MEASUREMENT REASUIREMENT SAMPLE MEASUREMENT REQUIREMENT, n, SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT
- REQUJIREMENT:;. . .- .
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE Richard T. Purcell direction or supervision in accordance with a system designed to assure that qualified personnel property gather end evaluate the information submitted Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the (
A Environmental Supervisor Site Vice President information, the information submitted Is , to the best of my knowledge and belief, true, EnvironmentalSupervir423 843-6700 03 12 13 accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I information, including the possibility of fine and Impnsonment for knowing violations OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Operations performs visual inspections for floating debris and oil and grease during all backwashes EP-PAF-orm ;3320,-1 (RElV 3199) Previous editionsmay be used Page I of 1
PERMITTEE NAMEADDRESS (Include Fac/lItY Nameltocationif Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No 2040-0004 Address P 0 BOX 2000
_LINTER O FFICýE S FB~j-2A.-)
- - TN0065 0 1 118 G F- FINAL SODDY - DAISY TN 37384 PERMIT NUMBER I DISCHARGE NUMBERI WASTEWATER & STORM WATER Faciitvy TVA - SEQUOYAH NUCLEAR PLANT Localion HAMILTON COUNTY SMONITO'RING EERIOD EFFLUENT YEIARMMO1 AI NO DISCHARGE ...
ATTN: Michael G. Beavers From 03 j01 011 To 03 01 31 NOTE Read instructions before completlnq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE OXYGEN, DISSOLVED (DO) SAMPLE *
- MEASUREMENT 19 00300 1 0 0 ,PERM!,,
URQUfREMENT *******';* ..... = < " .... )J-* ,
- MG/L P I E/.R, EFFLUENT GROSS VALUE 1
~w~ADIY T~i~~ MN ýt EE~
SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT- ** ** 19 00530 1 0 0 *k-- O>4 MGL - TiE GAZ EFFLUENT GROSS VALUE ýE
__F::__ V SOLIDS, SETTLEABLE SAMPLE ** 25 MEASUREMENT 25 00545 1 0 0 ",."A to ML/L iO E -. G RA.b, EFFLUENT GROSS VALUE *,i ,* NREQUITIEMENT, FLOW, IN CONDUIT OR THRU SAMPLE 03* **
MEASUREMENT 03 TREATMENT PLANT 50050 1 0 0 "* PERMIT*p C1,*REPORT'. RT.'r MGD . . .-- (E RE 0 RM~ e'IjCI",STM MEASUREMENT M0A" EFLUN GROSSE VAU 'DK SAMPLE MEASUREMENT SAMPLE MEASUREMENT
"-'REQUIREMENT~ - ~~~: i~~
SAMPLE MEASUREMENT 4 7 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision In accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted Based on my inquiry of the person or /
persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor information. the Information submitted Is . to the best of my knowledge and belief, true, 423 843-6700 03 02 13 Site Vice President accurate, and complete I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, Including the possibilty of fine and imprlsonment for knowing vlolations OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of 1
February 7, 2003 Richard D. Nunley, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, JANUARY, 2003 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101, samples collected January 12-17, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity, although survival was improved in the UV treated test.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN January 2003m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: February 7, 2003
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN 0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD): 3,266
- 7. 1Q10:
- 8. Outfall Tested: 101
- 9. Dates Sampled: January 12 - 17, 2003
- 10. Average Flow on Days Sampled (MGD): 1605, 1578, 1601
- 11. Pertinent Site Conditions: No unusual conditions reported. (Production /
operation data will be provided upon request.)
- 12. Test Dates: January 14 - 21, 2003
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Cotncentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint(%): Outfall 101: IC, 5 = 43.9%
- 17. Test Results: Outfall 101: Pimephalespromelas: IC 2 5 > 100 Ceriodaphniadubia: IC. > 100 UV treated Outfall 101: Pimephalespromelas: ICZ5_ > 100
- 18. Facility
Contact:
Richard D. Nunley Phone #: (423) 843-7748
- 19. Consulting / Testing Lab: Environmental Testing Solutions, LLC
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 862-8193
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22. Notes: Outfall 101, samples collected January 12-17, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity, although survival was improved in the UV treated test.
2 1
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/
Sample Time (EST) Time (EST) Temp. TRC* Time (EST)
ID Collected Received (CC) (mg/_) Used 101 01/12/03 1350 to 01/14/03 0941 0.7t <0.10 01/14/03 1517 01/13/03 1250 01/15/03 1500 Intake 01/12/03 1329 to 01/14/03 0941 0.7 <0.10 01/14/03 1517 01/13/03 1229 01/15/03 1500 101 01/14/03 1254 to 01/16/03 0956 0.6' <0.10 01/16/03 1426 01/15/03 1154 01/17/03 1431 Intake 01/14/03 1248 to 01/16/03 0956 0.3 <0.10 01/16/03 1426 01/15/03 1148 01/17/03 1431 01/16/03 1301 to 01/18/03 1430 101 01/16/03 1301 01/18/03 1000 0.7' <0.10 01/19/03 1502 01/17/03 1201 01/20/03 1436 01/16/03 1254 to 01/18/03 1430 Intake 01/17/03 1154 01/18/03 1000 0.2 <0.10 01/19/03 1502 01/20/03 1436
- TRC = Total Residual Chlorine
'Collected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival
- 4. Sample Manipulation: Samples from Outfall 101 and the Intake were warmed to test temperature (25.0 +/- 1 .0C) in a warm water bath.
Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Rainbow Lifeguardo UV Sterilizer for 2 minutes.
3
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatic BioSystems, Inc. In-house Cultures
- 2. Age: 24.0-27.0 hours0 days <br />0 hours <br />0 weeks <br />0 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 1
- 6) Test Initiation: (Date/Time)
Outfall 101 01/14/03-1450 EST 01/14/03-1517 EST UV Treated Outfall 101 01/14/03-1420 EST
- 7) Test Termination: (Date/Time)
Outfall 101 01/21/03-1440 EST 01/21/03-1504 EST UV Treated Outfall 101 01/21/03-1423 EST
- 8) Test Temperature: Outfall 101: Mean =24.8 TC Mean = 24.80 C (24.4-25.4 0 C) (24.3-25.60 C)
Test Temperature: UV-Treated Outfall 101: Mean = 24.7°C (24.3-25.3 0 C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted January 14 -21, 2003 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - da s)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22% 100 98 98 98 98 98 95 43.9% 100 100 100 100 100 100 98 72% 100 100 100 100 100 100 100 100% 100 100 98 90 85 85 83 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2 3 4 Mean Control 0.6888 0.580 0.620 0.676 0.641 10.98% 0.626 0.625 0.591 0.671 0.628 22% 0.624 0.703 0.707 0.633 0.667 43.9% 0.560 0.568 0.606 0.648 0.596 72% 0.627 0.561 0.652 0.677 0.629 100% 0.181 0.693 0.665 0.560 0.525 Intake 0.508 0.580 0.718 0.681 0.622 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9 95% Confidence Limits: NA Permit Limit: 2.3 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50 : TUc = 100/IC25 tOne animal missing. Replicate was initiated with 9 animals for statistical analysis.
5
TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted January 14 - 21, 2003 using effluent from Outfall 101.
Percent Surviving Test (time interval used - days)
Solutions 1 2 3 4 5 6 7
(% Effluent)
Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72% 100 100 100 100 100 100 100 100% 100 100 100 l0a 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (#young/female/7 days)
Test Solutions Data (replicate number)
(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 29 35 32 36 34 36 38 35 32 34 34.1 10.98% 36 36 33 37 35 34 42 34 39 37 36.3 22% 33 40 40 39 38 36 39 39 35 32 37.1 43.9% 41 37 34 41 40 40 38 39 33 36 37.9 72% 40 38 44 39 36 37 40 42 45 40 40.1 100% 42 42 40 38 40 38 39 37 43 39 39.8 Intake 37 34 40 40 40 34 34 37 37 39 37.2 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9 Permit Limit: 2.3 TUc 95% Confidence Limits: NA Upper Limit: NA Lower Limit: NA
- TUa = l00/LC5 0: TUc = 100/IC2 5 6
TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)
- 3. Results of a PimephalespromelasChronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted January 14 - 21, 2003 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions (time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72% 100 100 100 100 100 100 100 100% 100 100 100 100 100 98 98 Intake 100 100 100 100 100 100 100 Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2 3 4 Mean Control 0.532 0.686 0.720 0.603 0.635 10.98% 0.759 0.626 0.689 0.644 0.680 22%" 0.664 0.650 0.653 0.645 0.653 43.9% 0.702 0.576 0.556 0.634 0.617 72% 0.741 0.608 0.661 0.660 0.668 100% 0.624 0.706 0.686 0.619 0.659 Intake 0.699 0.632 0.652 0.669 0.663 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
Permit Limit: 43.9 95% Confidence Limits: NA Permit Limit: 2.3 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50 : TUc = 100/IC 25 Reference Toxicant Test Results (see Appendix A and D)
Species Date Time Duration Toxicant Results (IC 25)
Pimnephalespromelas January 14, 2003 1400 7-days KCI 607.9 mg/L Ceriodaphniadubia January 7, 2003 1200 7-days NaCl 962.4 mg/L 7
3/4 PIIYSICAL/CIIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelasand Ceriodaphmadubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, January 14-21, 2003.
Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH1 (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO 3) (mg/L CaCO3 ) Chlorine (mg/L)
Co'ntrol 245 24.9 78 7.4 8.13 7.84 295 62 85 244 - 24.7 246 - 25.3 7.6 - 80 64 - 81 806 - 8.19 762 - 8.11 278 - 308 60- 64 84 - 86 24.9 7.9 7.2 8.15 7.81 289 -
10.98% 246
- 248 246 - 253 75 - 85 62 - 80 805 - 822 758 - 803 278 - 298 S244 S 22% 24.6 24.9 8.0 7.2 8.15 7.81 279 -
S244 - 248 246 - 254 7.5 - 85 63 - 8.1 8.07 - 8.20 761 - 808 266 - 288
- 4. 24.6 24.9 8.1 7.4 8.14 784 259 -
244 - 248 246 - 254 75 - 86 63 - 82 807 - 8.19 764 - 809 246 - 266 72% 24.7 24.9 8.2 7.4 8.14 7 86 231 -
244 - 250 246 - 254 79 - 86 65 - 81 808 - 819 767 - 8.16 220 - 240 - -
S100% 247 24.9 8.3 7.5 8.14 7.93 203 71 88 < 0.10 244 - 252 246 - 254 78 - 86 68 - 80 807 - 818 775 - 822 196 - 213 68 - 73 86 - 89 <010 - <010 Intake 246 249 8.5 76 8.12 7.91 203 72 81 < 0.10 244 - 249 246 - 254 82 - 88 69 - 83 801 - 819 7.72 - 818 193 - 214 70 - 74 80 - 82 <0.10 - <010 Control 245 250 78 81 8.13 8.18 295 62 85 243 - 247 248 - 253 76 - 80 78 - 83 806 - 819 814 - 823 278 - 308 60 - 64 84 - 86 -
10.98% 246 250 7.9 8.1 8.15 8.19 289 -
245 - 246 248 - 253 7.5 -85 7.9 - 8.3 805 - 822 816 - 823 278 - 298 - - - -
22% 246 25.0 80 8.2 8.15 821 279 - -
245 - 247 248 - 253 75 -85 80 - 83 807 - 8.20 8.16 - 826 266 - 288 -
247 250 8.1 8.2 8.14 821 258 -
- 249 248 - 254 75 - 86 80 - 8.3 807 - 819 816 - S245824 246 - 266 -- -
72% 2458 25.0 8.2 8.2 8.14 826 231 - -
245 - 251 248 - 25.3 7.9 - 86 80 - 83 808 - 819 821 - 831 220 - 240 - -
100% 249 250 83 82 8.14 8.28 203 71 88 < 0.10 244 - 256 248 - 253 78 - 86 78 - 84 807 - 818 822 - 830 196 - 213 68 - 73 86 - 89 <0.10 - <010 Intake 24.7 250 8.5 8.1 8.12 8.26 203 72 81 < 0.10 1 244 - 249 248 - 253 82 - 88 80 - 8.3 801 - 819 822 - 830 193 - 214 70 - 74 80 - 82 <010 - <010 8
PlIYSICAI/CiIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the PimephalespromelasTest, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, January 14-21, 2003.
Test Sample ID Temperature CC) Dissolved Oxygen (mg/L) pll (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO3) (mg/L CaCO3 ) Chlorine (mg/L)
Control 245 24.8 8.3 7.7 8.11 787 299 62 85 243 - 248 245 - 251 81 - 86 69 - 84 8.03 - 817 766 - 808 286 - 309 60 - 64 84 - 86 10.98% 246 248 8.3 74 8.11 785 293 S244 - 248 245 - 251 81 - 86 65 - 82 802 - 818 763 - 807 283 - 300 246 248 82 75 8.10 7.86 281
- 248 244 - 25.1 7.9 - 86 68 - 82 804 - 818 766 - 807 273 - 286 246 24.8 82 76 8.10 7.87 260 43.9%
244 - 249 244 - 251 78 - 85 69 - 8.3 804 - 818 766 - 807 254 - 264 S7 72%
2% 247 248 8.2 76 8.11 787 231
- 25.1 244 - 251 78 - 85 69 - 83 805 - 8.18 7.67 - 809 226 - 235 S24.5 100% 24.7 248 81 76 8 11 7.90 203 71 88 <0.10 245 - 253 244 - 251 78 - 85 69 - 8.4 804 - 818 770 - 813 198 - 209 68 - 73 86 - 89 <010 - <010 Intake 246 248 82 7.7 8.10 - 7.91 202 72 81 < 0.10 1ntake 244 - 249 244 - 25.1 78 - 86 69 - 86 803 - 818 771 - 810 197 - 207 70 - 74 80 - 82 <010 - <0.10 9
SUMMARY
/ CONCLUSIONS Outfall 101, samples collected January 12-17, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
In addition to the routine compliance test, fathead minnows were also tested in Outfall 101 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality which occurred in minnows exposed to routine compliance samples and UV treated samples was not sufficient to jeopardize statistical validity, although survival was improved in the UV treated test.
10
. 4 Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30 mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None 11
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Total Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g KCl or NaCl.
Sodium Chloride (NaCl crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
12
REFERENCES
- 1. NPDES Permit No. TN 0026450
- 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).
- 3. Methods for Chemical Analysis of Water and Wastes, EPAI600/4-79/020 (March 1983).
13
Sequoyah Nuclear Plant Biomonitoring January 14 - 21, 2003 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-January 17, 2003 Date -,"Sodium, Towerbrom PCL-222,' PCL-401 CL363 Cuprostat- H-130M 1i~jochorite' PF mg/L TRC mg/L TRC .rg/L mg/L fmgi/L mg/L "'6g0L
. Phosphate,, Copolymer 1.DMAD; Azole Quat 03/12/1998 0.0 16 -
03/13/1998 0.01 ,
03/14/1998 -0.013, -,, -
03/ 15/1998 '0.0'30. -:
03/16/ 1998 ~0.013 03/17/1998 0.020 - - - -
03/18/19981 0.018 , .
09/08/1998 '0.015 - 0.014 0.005 - 0.021 09/09/1998 0-'0.003 ý'0.031/,,- 0.011 - -
09/10/1998 0.014 -0.060 0.021 . -
09/11/1998 -0.013 - ",0.055 0.019 -
09/12/1998 .<0.001 - 0.044 - 0.015 - -
09/13/1998 <0.001, - : '0.044 0.015 - -
09/14/1998 -0.008 0.044" 0.015 - . .
02/22/1999 '"<0.001" - - -
02/23/1999 0.005 - - -
02/24/1999 ,-0.009 - .- - .
02/25/1999 0012.0 - - - . -
02/26/1999 0.008'-ý ... ..
02/27/1999 <01, 0.00 - - ---
02/28/1999, < 0.001 - . - - - -. "
08/18/1999 0.015 0.069 0.024 ":0.006' 08/19/1999 0.012 , 0.068 - 0.024 -
08/20/1999 0 023 0.-6.076 0.024 0.120 08/21/1999 - 0.022 - ,,0.068 0.024 - "
08/22/1999 - - 0.022 "0.068 0.024 -
08/23/1999 0.025 10V068 0.024 0.006 08/24/1999 0.016 0.067 0.023 .0.020 -
15
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-January 17, 2003 Date Jf2
-,,!Sodiuin- -
y~c1P6horite
-mgLTRC,,
mg/L TRC j:..
Towerbrom 'PCL-222' TPh6sphte2 PCL401
-!Jg/ mg/L Copolymer
-L-363-, Cuprostat- H-130M mgfL DMAD mg/L Azole PFnmg/L-,
Quat 01/31/2000 - < 0.002 0.026 . 0.009 02/01/2000 ""0.011 .0.026 0.028 .....
02/02/2000 - 0.028 0.026.. 0.009 0.006 .
02/03/2000 0.008 0.027 0.009 . - "
02/04/2000 0.006 0.027 K 0.009 ::0.005 0.109 02/05/2000 < 0.002 0.027,-, 0.009 02/06/2000 < 0.002 0.027 0.009 . -
07/26/2000 -< ,,, <0.0057 *,'*,0.055 0.019 - - 0.021 07/27/2000 -,, 0.019 "': 0.055 0.019 - -,
07/28/2000 . -, 0.0088 0.053 0.018 0.004 0.108 -,
07/29/2000 - <0.0088
<, : -0.055*, 0.019 - - -,
07/30/2000 <0.0076 -0.055 0.019 - .
07/31/2000 < 0.0152 ,0.655" 0.019 0.006 "
08/01/2000 - < 0.0141 -,0.055 0.019 0.005 -
12/11/2000 , 0.0143 - 0.025 0.020 0.005 12/12/2000 - 0.0092 .0025 0.020 0.005 12/13/2000 . - < 0.0120 0.025 0.020 , -
12/14/2000 - <0.0087 [, 0.025 0.020 - -
12/15/2000 . - 0.0120 ;','0.025 - 0.020 !0.005 "
12/16/2000 - <0.0036 '0025' 0.020 -
12/17/2000, - < 0.0036 0.025 0.020 - '
08/26/2001 - 0.017 0.06 0.021 0.006 ,
08/27/2001 - <0.0096 0.06 0.021 '0.005 0.021 08/28/2001 - - <0.0085 0.06 0.021 - - ,
08/29/2001 , - , <0.0094 0.059 0.020 0.005 - 0.021 08/30/2001 ,' <0.0123 -1,-0.06 0.021 0.005 08/31/2001 - <0.005 '70.059 0.020 -
11/25/2001 - - <0.0044 - .-
11/26/2001 - - <0.0119 0.024 0.02 0.005 11/27/2001 , , 0.0137 0.023 , 0.019 0.007 -
11/28/2001 - <0.0089 -, 0.022, 0.019 '0.006 b
11/29/2001 - 0.0132 0.024,-" 0.02 0.007 11/30/20011 < 0.0043 0.024 "- 0.02 - -
12/09/2001 "
- <0.0042 - . - *
- 12/10/2001 -' <0.0042 - , ,: - " "
12/11/2001 - <0.0104 , . _ .
12/12/2001 - 0.0128 0.024 0.02 0.008 -
12/13/2001 <0.0088 0.024 . 0.02 - -
12/14/2001 0.0134 '0.024 0.02 0.007 - -
16
Table B- I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-January 17, 2003 Date S~iu .",I Towerbrom PCL-222? PCL-401 Curstt PCL363 o H-3OM mg/LTRC mgf m/Azole Lmg/L
.__.....___ ..... __,Phosphate Copolymer DMAD mg/LAzoe 01/02/2002 <0.0079 - 0.023, 0.02 -0.006W 01/03/2002 < 0.0042 o.02-3, 0.0144-,
01/04/2002 0.0124 024. 0.014 000 01/05/2002 1-, < 0.0042 7 01/06/2002 < 0.0042.... .. "
0.1/07/2002 ... _ < 0.0089 -,;' 0.024 "ýrý 0.014 0.006 "
02/24/2002 - , <0.004 02/25/2002 < 0.004 0.023 0.023 02/26/2002 1 0.0143 0.023 0.023 0.007 02/27/2002 < 0.0041 K 0.023 .023 - ,
02/28/2002 <0.0041 "-,0.024 0.008 - -
03/01/20021 - . <0.0041 -,;0.024 0.008 - .
05/05/2002 ._ ,
05/06/2002 0.058 0.02 '0.014 05/07/2002 - 0.058" 0.02 --0.015 05/08/2002 0.056 0.019 - ,
05/09/2002 0.057 0.02 00 14 05/10/2002 . 0.056 0.019 . 1, 08/04/2002 7 <0.0058 08/05/2002 <0.0058 '0.053: 0.018 ,0.025,'
08/06/2002 j . 0.0092 - 0.053 0.018 08/07/2002 %. <0.0107 " 0.055 0.019 0.007 08/08/2002 <0.0061 0.055, 0.019 -*
08/09/2002 _ __ , 0.0152 . 0.054 0.018 0.008 10/06/2002 <0.00497 10/07/2002 - 0.0153 0.054, 0.018 0.009 10/08/2002 ' - <0.0092 '0.054 0.018 0.007 10/09/2002 0.0124 0.053 0.018 0.009 -
10/10/2002 - 0.0134 0.054 0018 0.009 -
10/11/2002 1 - <0.0042 0.054 0018 -- -
01/12/2003 - <0.0035 - - -7 -
01/13/2003 - <0.006 0.025., 0.019 0.009, 01/14/2003 , - <0.0118 o0.26 0.020 -
01/15/2003 , - <0.0063 0.026 0.020 0.009 -
01/16/2003 -, - - <0.0034 0.026 0020 :
01/17/2003 <0.0034 0.026 0.009 '*- - -
17
Sequoyah Nuclear Plant Biomonitoring January 14 - 21, 2003 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1.._ of kJ Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Research Building ED UPS Bus Client Project Name: TVA SQN P 0.351 DEPOT STREET Other (specify):.
ASHEVILLE. NC 28801 Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-350-9364 General Comments:
Fax: 256-386-2963 - C1- .. ' i, NPDES Number: 0026450 Collected By: WANDA ALLEN Container Field Identification I Grab/ Ship. Collection Date/lime Number & Flow Rain Event? Laboratory Use Volume (MGD) (Mark as Appropriate)
Sample Description Comp. 0C)(
Temp Collected I e______
P='/
Start End .. , Y- If Yes. No Trace EST Log Amval Temp. ( By Time Appear 0
-- Inches Number C) ance SQN-101-TOX comp 4 1/12/03 /9 2(2.5g) ,, 0501 N. 0I o,"c "C,,"
i 51 , 1/13/03
- SQN-INT-TOX comp 4 1/12/03 1(2 5g) 0 1t1qI.. O.1"C
- ___
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time
- **.4 am,, 1/13/03 100 "eboi-'l'0-1 s IG
-etc O0iq-oA 3 0i__ I -0 .-o0 cA -41 0
Instructions- Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4 C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samplce shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page --I of A1 I
Environmental Testing Solutions, LLC Delivered By (Circle One):
Client: TVA A, Research Building EE UPS Bus Client Project Name: TVA SQN 351 DEPOT STREET Other (specify):.
P 0. Number: PO BOX 2000 ASHEVILLE. NC 28801 Phone: 828-350-9364 General Comments:
Facility Sampled: SEQUOYAH NUCLEAR PLANT Fax: 256-386-2963 ~O~A.AfAL~~A ooL took ~-
NPDES Number: 0026450 Collected By: WANDA ALLEN
- Y - ! -
_____________________ I-Field Identification / I Sample Description 1
SQN- 101-TOX SQN-INT-TOX
- qn rnhe Ciu~todv -- Fill In From Ton Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time
--- 1/15/03 Sa Cutd ilI rmT*Dw 00 to....
0 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitonng samples are preserved by storing them at 4 C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Research Building IEx UPS Bus Client Project Name: TVA SQN P.O. Number: P0 BOX 2000 351 DEPOT STREET Other (specify):
ASHEVILLE. NC 28801 Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-350-9364 General Comments:
NPDES Number: 0026450 Fax: 256-386-2963 A. C.to*°a1 a -"
ALLEN Collected By: WANDA Container Field Identification I Grab/ Ship Collection Date/Time Number & Flow Rain Event? Laboratory Use Sample Description Comp. Temp ( Volume (MGD) (Mark as Appiopriate)
°C) Collected _____ _-___" _t__"__
End Y. If No "riaCe EST Log Arrival Temp ( By Time Appear X!X?- .Ys, Soart
-e.& ,_, -jf'f'13/ 1/17/03 Iniiws Number *C) 0'"j-IC "Lt11 *see ance SQN-101-TOX comp 4 1/116/03 _0IZ1 2(2 5g) RI Io-SQN-INT-TOX comp 4 VIP10 3 1/17/03 1 (25g) 0. C 10is.0 tool)
Sample Custody - Fill In From Top Down Relinquished By (Signature). Dlterl'ime Received By (Signature): Date/Time 1/17/03 /6omVol- 01-1"Ol 0 ,10 j/,,*4td4 d Fe2,Oi1-O, IO _________________-1t-3 UO mzzz101 them in ice The hold time for each sample Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Binmonitoring samples are pieserved by storing them at 41C and shipping please collect and ship in such a way that the laboratory will ieceive the samples with ample time to initiate testing within that time frame Samples is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday
Page - Gi --
V ;v- nvrintafl Tpct~n .4nl,,tinnc TT.CPae.L Sample Receipt Log Date Time Received Received Sample Project Sample Receied ByFrom Rceivd Temperature ubrNme Sample Name and Description State Comments c~~d~FeO3 lo4 O-'. 600 0 0c1- sT-'" FAP 6 t-oq-o 1j)jS (U"o-ý Fejzex 0.C 510 03ol101. 13TeSrT'iO 10& ___
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01-ig-O--, U)4 YE&,A re>P)- _____ .VC -571 4 "'kep e.,- CopagITe.. Z ___
OI-15-03 100Q3 X(EKeetW piR-t- e 1.0C- LýL 9301115. 01CIOP NQ1eAK. ShI60 I___
01- 15-03 1005 VE)<eelAr-J 6"cqj~txJt 0 .4C ;515 o-6,ovis.oz T-Aee K's 1 RiA-P M JW-4 býVeJ* EtNea4-4 C-OfP.MC 01-1-i> KeV-eeA4'd Feb ex O.SC 'S1( its1IS3 A Lte# S P-A R 'STVA-M __W 01-.c 6- lozL 1021 KEleeAMb l3Ke. 0(A 'C 157 05c)tI's0L TVOA NC.-sct_________'
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Environmental Testing Solutions. LLC Page 1e.2..
Sample Receipt Log Date Time Received Received Sample Project Sample By From Temperature Number Number Sample Name and Description State Comments Received Received 0~.zt.~3 ~O 0 .iC 511 ~~oi TA- S~e&%.U&AA MOtCLA~f- fLASr 011902 100Fbe4 .-- 61 OotI -0I OtITFALL 161 T74 o1Ilt-03 loc~o _ 0.2*C. 567 03wl02. TV-S-mMMUL49Am 1 o~~~~~tt-o~~~~~~~~~ .C *I 0o1.3 e-Oix~'mj rJWr VE-eAW )
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M-22-0 I O'i*3 KEKbe"~,v~ ~A ebe o-ec -C 6- 30122~.01 A'~nAs LtAn-ASSL(6,I A -vU- t&XF~ Ac~res PilAse M11 ot~-W-3 i t 3(. KEKWNQAý f:eIJO.V O.CV'C 59O oZýOt 2 q. okI T--r I-F16t 01-2+A03 w1W. KEKee,-Ai. Fe-bey- OAVC 15o 0-50z1.O24. 02 -ýr 1A-F 0 1~z-c2L-& 113(, KE Keef,ý v Fetýe-g 0______ -5. 0o IoIot -a3 Tesr -1 01-21403 1t1t5, KEK~e#.jAk% Febegi 0.9V 690 0-?.014 Tesr s_ ____
,0124d-3 116 je.)At V FeP- O.9 C 6f 0o-ý a Iz'. os Tesr Is 3 01-2q-03 11,3L KE eetAN,~ O9A ,C 590 0501-2qf-01 Iesr 0l-2q.iO5 IIZk' KFK~ee,ýMA t.., ____/ 0.9,C 69 --8q offr -1_
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6j-zq-63 1136o KEaeetiAq4 F:X 0.9 *C Sr Io~oiz . 16 TesV .3 -'aw tiQ/ I_ ____
- Environmental Testing* Solutions, LLC Pa*e 1 off Evom tle I S s LPam 1 of.6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: "VIPk Facility: 5e,&%,eoIA &IgePw*_ PI NPDES #: -Tv o6i2.LQQ Project #: 6-11 Dilution preparationinformation: Comments:
Dilution prep (%) l7 22. '7o' iL .. l __
Effluent volume (mL) I* 53l56-'2 Cotoe L-500 Dfluentvolume(mL) lb35 )l-70 Vqg.S 42-o 0 Total volume (mL) 9 CO I SCo IS'M: 15M __,_ _ _ _
Test organism information: Test information:
Organism age: 24, tk-tý. Randomizing template: Gg-,
Date and times organisms Oa-%s-ot 1"21A'To %S50 Incubator number:
were born between: t'" _
Organism source: N*s 6NTeCj 014'-G 3 Artemia lot number: S&1RCA{
Transfer bowl information: pH = Temperature = °C Total drying time: I1.Z*.$. ,
"7.3(o ZcA. Z Date / Time in:
Average transfer volume. q Date / Time out: n" ba I , Oven temperature: 16 I Dailyfeeding andrenewal information:
Day Date Morning Afternoon Test initiation, Control water Sample numbers Analyst feeding feeding renewal, or batch used used time time termination time _ __ _
_ __ I __
0 ---
____ 1- IA45 KHMSOV.2-1LO' SO1 OSWACA A I a1-sc0, Oq 14 Is-e -o MAS.G-I-z.o5 0-3011.6X 05o~I4q?.
2 0AittOt ON 1- 1,tS 1%AS -0c*5 Q*111.O61 O0oll5,116 3___ M1-l1 -Oot 3S 3(0 IS gac piS-3 oi- 6501%6.0% 6-3091V 4 VI-06 1 to lII*1;40 ms i 03011k A6St
-.. .- qJ - , I 1/4 IJ *.J IIIlt
- l II* ....al L]-'II:I I It*t_* [Ji * [ff*l I*
7 IrN - ~'~'lV" -o ""- A l.-
0I21.b3 Control information: Acceptance ertena Summary of test endpoints:
% Mortality: cf7. <20% 7-day LCso > 100%
Average weight per initial larvae- O. I1 NOEC 10__07_
Average weight per surviving larvae: 0.6qI >0.25 mg/larvae LOEC > I______
ChV IC25 [ > op%
-' Environmental Testing Solutions, LLC Page 2 of 6 Species: Pimephalespromelas Client: fop d, pA. t.-[-z*0 Date: - .
Survival and Growth Data Day CONTROL to Z7 A B C D E F G H I J K L 0 1/0 /0 (0 /o 0 /0 /0 /0 /0 /0 /0 10 1 o1 10 1o 16 1I 10 !0 10 /b 10 /11 /0 2 /o /0 to (0 /0 )0 /0 /0 t0 /0 A)
/1 4 3
_ __ 1.0 g / to0'- A3I X) 93 1 4 0 o to 1o to 10 kO 10 Wo /0 to 97 5 1o 1 10 /a1 /6 (0 /0 10 1/0 /0 10 6 to (6 /0 /0 /6 /0 /0 /0 /0 /0 10'j' 7 q~ iSi4AUL 1
_ _. _ (0 _ 10 10 0 t10 ) /D c to /0 A -Pan weight (mF) b 6___ 0" %0 \DO-B = Pan + Larvae weight (mg) * \ 0, 4 c 0.1 *bA Lar ,*e-ht (m g -A-B B. . . . .a ., ,. 1 . , -AID o o 0. 1b s I.,
Calculations and data reviewed Comments:
.. Environmental Testing Solutinnq.. TIC SEnvironmenta Testing S n. .. r rage 3 of 6 Species: Pimephalespromelas Client: L,&O'-qo.AI - TgoA-r eb Date: 01- 1+0'3 Survival and Growth Data Day - Sj'T A2%I0(*
M N 0 P Q R S T U V W X 0 / 01/0 10 1/0 /01/0 /0 /0 /0 10 0 2 /0 1b 16 10 /I) 10 1/ 0 16 /0 16 4 10 I1) to0 /0 ko 10 10 /0 /o 10 10 5 10 Io / 10 /0 10 10 I/0 cJ4 /0 10 6 Io (0 0 lo ,o /0I to 1b - I a /o /0 70 10 /0 to0 (0 !/0 1,4 7V' i0 A-Pan weight (mg) p # \c4'~) 6 1 (c' '0 6 *"
B- Pan + Larvae weight (mg) 0 6Vý AA Y acfý ~'LOA,16 ,a w~ '2I0" 7
.011 Larvae weight (mg) -A-B 6c ~OVA~ttt ~~l ,A~.-t"~ ~ ~
Calculations and data reviewed Comments:
PO_,CkQ%2&.A.A_ aA j)j,&J, a&AAý heaA LO-0 U
J"OU 4 U %.j
Environmental Testing Solutions. LLC
.- ar',+ ofU Species: Pimephalespromelas Client:
- Q*ooNPdA - &WqreAT Date: ol-l'A-oA Survival and Growth Data Day IrNi"iKe Y Z AA BB 0 10 /0 to (0 11 /0 10 10 2 /0 /0 /0 /0 3
/06 16 10 A 1( /0 (0 /0 6 i/!) I0 o /t 7 514ra 110 IO to 10 A -Pan weight (mg)
B- Pan + Larvae weight (mg) %0 0 Larvae weight~m -* - B . - 1.-
Calculations and data reviewed:
Comments:
Environmental Testing Solutions, LLC
-'i Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client TVA Sequoyah Nuclear Plant, Nontreated Test dates: January 14 - 21, 2003 Revetwed by: ______________
Project number. 571 Coneentratlon () Repcate lnltial number of FlaIt number ofilrvat A- Pan weight (mg) B - Pon
- Lame Larve weight (mg) Weigh/lntianmbero Mean survival Mean weight(rng) -coefent o Percent reduction from larvae weight (mg) - A- B lame (mg) (V/) variation (%) control (1A)
A 9 9 14777 20970 6193 0,6881 Control B 10 10 14647 20450 5803 05803 1000 06411 78 Not applicable C 10 10 15049 21250 6201 06201 D 10 10 14832 21590 6758 06758 E 10 10 14708 20970 6262 06262 10 9811/ F 10 10 15076 21330 6254 06254 1000 06285 52 20 G 10 10 15097 21010 5913 05913 H 10 10 14990 21.700 6710 06710 1 10 9 14834 21070 6236 06236 22% J 10 10 14691 21720 7029 0 7029 950 06665 66 -40 K 10 10 14855 21920 7065 07065 L 10 9 15039 21370 6331 06331 M 10 9 15150 20750 5600 05600 439% N 10 10 14690 20370 5680 05680 975 05956 68 7.1 0 10 10 14976 21040 6064 06064 P 10 10 14869 21350 6481 06481 1 Q 10 10 14819 21090 6271 06271 72% R 10 10 15148 20760 5612 05612 1000 06292 79 19 S 10 10 14992 21510 6518 06518 T 10 10 14714 21480 6766 06766 U 10 3 15013 16820 1807 01807 100% V 10 10 14989 21920 6931 06931 825 0.5248 450 181 W 10 10 15340 21990 6650 06650 X 10 10 14666 20270 5604 05604 1 Y 10 10 15017 20100 5083 05083 100%Intake Z 10 10 14685 20480 5795 05795 1000 06218 154 30 AA 10 10 15129 22310 7181 07181 I BB 10 10 14716 21530 6814 0 6814 __
Outfall 108" MSD = Mrniunt Significant Difference Dunnett's MSD value: 0.1781 PMSD= Percent Mrinnim Significant Difference PMSD 27.8 PMSD is a rneasure of test precision The PMSD is the mininam, percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Envronmental Testing Solutions, LLC Intake chronic toxicity tests when a toxicant reduces Pinephales growth by 17 90/a from the control (deterrnned through reference toxicant testing)
Dunnett's MSD value: 0105 Lower PMSD bound determined by USEPA (I 0th percentile) = 9 40/
PMSD 164 Upper PMSD bound determined by USEPA (90th percentile) = 35%
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pmrrephales growth in chronic reference toxicant tests USEPAý 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Ehlimnation Program EPA-833-R-00-003 US Environmental Protection Agency, Cincinnati. OIL
Environmental Testing Solutions, LLC
°. Statistical Analyses Larval Fish Growth sad Survival Test-7 Day Growth Start Data 1/14103 Tedt ID- PpFRCR SampleID- TVA. Sequoyah Nuclear Plant End Data 1/2103 Lab IDM ETS-Eny.Tueng Soilntc Sample Type DMP-D-hVarge O-on Report SampleDate Protocol. EPAF 91-EPA Fnw,,&ter Teo Spec"s PP-Punephales promelas Cosnmathr CoD-% ! 2 3 4 D-Control 06381 05803 06201 06758 1098 06262 06254 0.5913 06710 22 06236 0 7029 07065 06331 43.9 05600 0-1630 0 6064 0.6481 72 06271 03612 06518 06766 100 01807 0.6931 06650 03604 Transform Uatrarasorzmd Rank 1-Tailed Isotonic Coenc% Mete N-Mean Mean Min Max CV% N Stm Critical Mean N-Mean D-Control 06411 10000 06411 05803 06381 7.326 4 06454 10000 1098 06285 09803 06255 03913 06710 5201 4 1700 1000 06454 10000 22 06665 10397 0.6665 06236 07065 6643 4 22.00 1000 06454 10000 43-9 05956 09291 0.5956 03600 06481 6787 4 1300 1000 0 6124 09489 72 06292 09314 06292 03612 06766 7886 4 1700 1000 06124 09489 t00 05248 08136 03248 0 1807 06931 45045 4 1600 1000 03248 03132 AnxilllarTestsat Statistic Critical Skew Kart Mapiro-Wdk'e Teat mcdcatee non-normal d&tnbuttoa (p - 0 0 2) 0785154283 0884 -2002403144 8376526546 Bartlett's Test mcatee unequal vanances (p - I 69E-03) 192979126 15 0631706 Hypothetis Test (1-tall 0.05) NOEC LOEC ChV T")
Steero Many-One Rank Teat 100 >100 1 Linear Interpolation (80 Resamples)
Point % SD 93%/aCL(Exp) Skew IC0S 43441 ICI0 32093 ICIS 92407 10 IC20 >100 09 IC25 >100 08 IC40 >t0o IC50 >100 07.
it 06.
-05.
904 W 03 02 01 000 - °
-01 a 20 40 60 80 100 120 Dose %
DoRt-ponze Plot 08 07 06 004 00o3 02 01 0
ao4 sqn 01-44-o~data~xl.
.1
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date 1/14(03 Test ID, PpFRCR Sample 1D TVA. Sequoyah Nuclear Plant End Date: 1/21/03 Lab ID ETS-Eny Testing Solutions Sample Type DMR-Dtscharge Monutoring Report Sample Date Protocol EPAF 91-EPA Freshwater Test Species PP-Pumephales promelas Comments COc-% 1 2 3 4 D-Control 10000 10000 10000 10000 l0.98 10000 10000 10000 10000 22 09000 10000 10000 09000 439 0.9000 1000 10000 10000 72 10000 10000 10000 10000 100 0.3000 10000 10000 1000 Transform- Arcsin Square Root Rank 1-Talled Cont-% Mean N-Mean Mean Min Max CV% N Sum Critical D-Control 10000 10000 14098 14033 14120 0.307 4 1098 10000 10000 14120 14120 14120 0000 4 2000 1000 22 09500 09500 1.3305 12490 14120 7072 4 1500 1000 439 09750 09750 1.3713 12490 14120 5942 4 17.50 1000 72 10000 10000 14120 14120 14120 0 000 4 20 00 1000 100 08250 0 8250 12039 05796 14120 34569 4 17.50 1000 Auxtilary Tests Statistic Critical Skew Kurt Shapmo-Wi`is Test indicates non-normal distrbution (p <- 0 0 1) 0 671914518 0884 -2 675882645 11.2806056 Equality of vanance cannot be confim-ed Hypothesis Test (I-tall, 0 05) NOEC LOEC ChV TU steers Matny-One Rank Test 100 >100 1 sqn..O-14-O3data~xls
Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation Only Larval Fish Growth and Survival Test-7 Day Growth Test ID PpFRCR Sample ID TVA. Sequoyah Nuclear Plant Start Date 1/14/03 Lab ID ETSEnv Testing Solutions Sample Type DMR-Ducharge Monitoring Report End Date 1/21/03 Protocol EPAF91-EPAFreshwater Test Species PP-Purnephsles promelas Sample Date Comments Conc-% 1 2 3 4 D-Control 06881 0.5803 06201 06758 1098 06262 06254 0.5913 0 6710 22 06236 07029 07063 06331 439 05600 05680 06064 06481 72 06271 05612 06518 06766 100 01807 0 6931 06650 05604 Transform: Untransformed 1-Tailed Min Max CV*/% N 1-Stat Critical MSD Conc-% Mean N-Mean Mean 10000 06411 05803 06881 7826 4 D-Control 06411 06710 5.201 4 0 171 2410 0 1781 1098 0 6285 09803 06285 05913 07065 6643 4 .0344 2410 0 1781 22 06665 10397 06665 06236 0 5600 06481 6787 4 0615 2410 01781 439 05956 09291 05956 06766 7886 4 0161 2410 01781 72 06292 09814 06292 05612 06931 45045 4 1.574 2410 0 1781 100 0.5248 08186 05248 01807 Statistic Critical Skew Kurt Auxiliary Tests -1002403144 8.376526546 0785154283 0884 Shapr-o-WIks Test indicates non-normal distribution (p <, 0 01) 192979126 1508631706 Bartdetts Test in,.icates unequal variances (p- 1 69E-03) df ChV TU MSDu MSDp MSB MSE F-Prob Hypothesis Test (0-tall. 0 05) NOEC LOEC 1 0 178087756 0277794306 000978056 001092104 0-.50879713 5,18 DunrnetlsTest 100 >100 sqnO.1-14-O3daftLxls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Crowth and Survival Tet-7 Day Crowth Start Data- 1/14/03 Test ID PpFRCR Sample ID TVA, Sequoyah Nuclear Plant, Intake End Date 1/21/03 Lab lDf ETS-Env Teting Solutions Sample Type DMR-Dscharge Monitoring Report Sample Date Protocol EPAF 91-EPA Freshwater Test Species PP-Pirtephales proraelas Comments Caesc-% 1 2 3 4 D-Control 06881 05803 06201 06758 100 0.083 05795 07181 06814 Transform, Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N 1-Stat Critical MSD D-Control 06411 10000 06411 05803 06881 7826 4 100 06218 0 9700 06218 05083 07181 15396 4 0-356 1943 0 1050 Auxiliary T"est Statistic Critical skew Kurt Shapro-W'ilks Test indicates normal dLstnbution (p > 00 1) 0963339746 0749 -0281148021 .0961324515 F-Test indicates equal vanances (p - 0 32) 3641183138 4746834564 Hypothesis Test (1-tall, 0 05)
Homoscedastic tTest indicates no significant differences sqn.Ol-14-O3daztaxlv J
Environmental TestinR Solutions, LLC Page 1 of 7 Enirnena TsII1 SouinLC ae1o° Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia Client: "T(*A Facility: 6et%3.4Al I MtX pA9 eLAt'-V NPDES #: TuJ 0O2Aj.5O Project #: 571 Dilutionpreparationinformation: Comments:
Dilution prep (%) '2s.' 12. 1_
Effluentvolume(mL) I4q.77 0 . / 0 I Diluent volume (mL) L1_a6. 3 z lIog (o/)51500_
Total volume (mL) 15CO 5
- o 1 SOO ISOO /Isa Test organism information: Test information:
Organism age: <*zq-AO4V% Cp.r Randomizing template: 64A&V Date and times organisms 01-14-6-6 orim -M tT.,q Incubator number and were born between- shelf location: ___
Organism source: 0 l-0-o7 -6 A-- YCT batch: At&~ .Z1-o24 Transfer bowl information, pH = E.21 Temperature= ZS. 1C. Selenastrum batch: A& a-25-02..
Daily renewal information:
Day Date Test initiation, Control water Sample numbers Analyst renewal, or batch used used termination time to IK e _____
0 ol-4 -o *3 __ _-__ 1 lilt 1 1-2-0503 CI3ol4.o o B0uN . .
I VSc.O 01_%2O2'J o 031' ao4.
2 o1-2 *A,-O . L,0, 0oo011'..c 053ot0 ,,.1 6*-1.-03 I 4A *51l,44 01-1t5o3 t3ol I*.O1 05o~t.
6 0oý o2-03 0 II4ASO *'.Ov,-t3 6361A.0%~o 0,o1%.O 2.3 7 o\ * - 'st 0 "D*,
Control information: Acceptance critena Summary of test endpoints:
% of Male Adults- :520% 7-day LC50 > I Wt.
% Adults having Yd 3 Broods: 2,80% NOEC 100vto
% Mortality: 520% LOEC > I-()1 Mean Offspring/Female: 15.0 offspnng/female ChV > lwcr'
% CV: j <<400% IC25 > 10607.,
Environmental Testing Solutions, LLC Page 2 of 7 Species: Ceriodaphniadubia Client: *,*cI~q Akl Date: oi-N-c),A
- rV.Ae CONTROL Survival andReproduction Data Replicate number Day 11 2 31 4 15 16 7 8 9 10 1Young produced Adult mortality 0 1~Q C)
X-i 0
L' 1
I L- I t-1 2_ Youngproduced I0IeI& F 0 C:2__ __
[ Adult mortality J L.I
- IL 3 Young producedII
__f Adult mortality Q1" I LI 1 -I I I I L-V I L 4 .YomgAut mortaity produced JLj(I 1z L- I\_IJ\ 1 j I -13110 iX- [_S-C)I-SI24110 I ~-I'I 3 L, T Young produced 0 1 C, i 101 01CL1 1_
Adult mortality J _* -It- ..
1-. K* -I 1 V.
Young produced 1( C). 0J 10J2 C W"A~.AIOve-'.~
Total young produced -z'9 3s ~ 3 c4~t 3%,o -:: vas X for 3 Broods I .2 ."
Concentration:
% Mortality: I . 0ýA,6 Mean Offsprin/Female:
CONCENTRATION: Survival and ReproductionData Replicate number Day 1 2 4 5 6_3 7 8 9 10 Adult mortality X _ -_ L fl 2
i Young produced Aduli mortality I 0
tX--LILIL IL -IL ZIL kL 3
__IAdult Young produced mortality Adult mortality JLL L L a
~
_1_ __
l I I_-
.~
L__
I LI_
C L~
5 Young produced Adult mortality I '*1 I'-.1 i 6 Young produced C_) 0icV 4o 1M 1 or L T~
[ Adultmortality L_ L-1 L1 cjIj I I 7 [Young produced q J I 1& 1 L 0 2i 1' 2 I Final Adult Mortality-- L.L.. LL. L L.- I Concentration:
% Mortality: 617.
Mean Offspring/Female: 1560.b
% Reduction from Control: -5.fV
Environmental Testing Solutions, LLC Pane 3 of 7 Species: Ceriodaphniadubia Client: 15L)ONAIl- 0tgrgeT:* Date: 0o1- It-c1 CONCENTRATION: 2Z2'go Survival andReproduction Data Replicate number Day 1 .i2 3 4 5 6 7 1 9 10 I Young produced 0 0 0 Adult mortality I I L 0.
Adult mortality ___ _l-I+ Ij- 1 3
2 4
__IAdult Young Young produced produced mortality Young produced I1 0 [LC IJ~jt..7jLIL.._~~I1IL IZ LI 9 C
11
)
T 0
) 1..
o 1 13..t 5
Final I Young produced Adult mortality . L._
1-5 1
. t IS i
I_
o
Ž1+/-5' J-L_ 11..
6 Young produced] 0lo_5c 1a
__ Adult mortality iL L ILju LJ . L ILtL1 7 Young produced 1-1 -Z _ 2 C) fi 27- Lzo -Z o 0 1 Total young produced ab LA) 0 '-t ai kI_ 5(. -= 3I 3' 5 Final Adult Mortality . -%. %..-
. ~ =.. -% -
Concentration:
% Mortality: 017 Mean Offspring/Female: 3 -"1I
% Reduction from Control: - 9. 11" CONCENTRATION: 4Survival andReproductionData Replicate number Day 1 2 3 1 4 5 6 7 8 9 10 I Young produced 0C n n (n 0 0 1 0 0_
Adult mortality L L 2 Young produced Adult mortality-0 Q.
CL+ oI
- t. -i L__
-"L L-L.-
O) .
I L.
° L--
0 (
L. ..,
1 L.- I L---
L.
61
._ {
I
°..
3 Young produced I Tjl l ,o It L I WVnI tl 17 l1 Adultmortality Lc_. L_ _-i 4 IYoun* p rduced u(edj{_I+/-L 7
___Adult Adult mortahity Young produced mortality L 1IL- LI-t- 12-1 i1 I 14L_ 15 1 15 Q1 L-1 I %--I Lj ItL_1 L_
L- I L--1 L I
l .
6 T Youngproduced Adult mortality _
10 1 0I 1Qqo o m lo b
_L..-L- L_ U._L._ . _
7 [Young produced 12 z L zCtT ~.1.
-L .
Total young produced qr4 13-51 i i3I 41 q'-C) qC ~ 3 .39 3-6 Final Adult Mortality L- I? L? %. -I: L C1 Concentration:
%Mortality: Q'7.
Mean Offspring/Female: 317. q
% Reduction from Control: -10.Sla
Environmental Testing Solutions, LLC Page 4 of 7 Species: Ceriodaphniadubia Client: 6*&%r,/AA - o 4'Rtx e Date: 0\---3 CONCENTRATION: Survival andReproductionData Replicate number Day 1 2 3 1 4 5 6 1 7 8 9 0 Adult mortality (w [. ..... L... U_ ___ .- U.. .
1 Adult mortality Young produced ~si' C C __ ) '-I 3 Young produced Q C) ( 01 1 G Adultmortality . _ \- '- - - I I-1 1 0I1 .
'- II '.I -I 3 Young produced - 1 01 1
_ mortality I IlAdult LA L.11LJI 4 Young produced [.3 ii "i,i. 3
- __ Z X.I c 1 Zi q S Adult mortality L f_.... j. - j_ -I \.- .- I -_
6 Young produced __ t mortality iAdult 6 YoungprOduced 6 n an RS Total young produced 38 qq I 5_ 3 1.3-i* 'L*)Iz 045 40 Final Adult Mortality - . I_ .- -. %. ._.
Concentration:
%Mortality: 0"7.
2Mean Offsprngfonemale: 'I. t I I
"% Reduction from Control: a- &I CONCENTRATION: -0OSurvival and Reproduction Data I ___________________T______
__2____ Replicate number ________ _______ _______ _______
1 Young produced r1f3 II () In i c D AY d o rtduit L-1 L-2- [ lI L I 6- - 7 I- 10o 2 Young produced I Q 1 U[ 0 C) Io1l C I C 61 r Young produced O I C) C (a[on:
Adult mortality 4 Young produced 7 1 5SL T1 ~(4) 1~~ I~ +/-
Adult mortality ___ I l'- I 'I'---I' X- I I F Adult al Adult mortality mortality II*~ L.- I L._ I L-L**** I12. ._ - I L..-
L.. .--L.. - It '%.SIL Young produced 0 0 0_Lj 2-0 1 qj ~ o'
__ Adultmortality U- __ L_ L___ ___ __
6 Young produced 7--2 L I IL/P 1ZI I' 1 -2 Total young produced qz c 2.q1 j DqoCe_ 3g 3193- 3 Final Adult Mortality - 1t -7 L Concentration:
% Mortality: h Mean Offspring/Female: 3'o
% Reduction from Control: -l(,, O*
Environmental Testing Solutions, LLC Page 5 of 7 Species: Ceriodaphniadubia Client: 5b)OMA" - tINTEP.Areb Date: dl-I-+03 CONCENTRATION: ICr, jMIM<Q Survival and ReproductionData Replicate number Day 1 Young producedi )
1I 2
(
13
(
I45 6
- (
7 8
9
(
10 Adult mortality _ _ ___ __ _. _ , 1 10 o 1"I C0. 1 0.. 0C1 I*<..CI CI"I~o lO ..c"I*
I~
L- I 2
1 Young produced Young produced I Adult mortality I I~uL.L4~~I~~~
L-4JA-- It-Toa yjoung produced L.cjCL.oj c Ic L . .I." " . c-a dlAdul t mortality .- I_'. [IL.. _'.. . .
- I 4 You~g produced~ '1_ TT. L LALŽ1V Young produced '3"1..j..1V 1.1:1' 1i1213
__Adult mortality -K ]'-I-I-viV i-1 6 Young produced Ic 1Yo
__ 00 Lk 1_ TO *,j C.AgUJ Adult mortality
]Young produced I -i:---I~
IOzoisj ICJo120
~ I 1-7J Wet 7 :1. 21 J22 Total young produced I_1 -t.I .40 L-o 4o 34 3-1 3I Final Adult Mlortallty L. L- L.. 7 -C72L4 t Concentration:
% Mortality: 0 I Mean Offspring/Female: 3"7.2.
% Reduction from Control: -. J
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dates: January 14 - 21, 2003 Reveiwed by: e*-
Project number: 571 ( A Replicate number Survival Average reproduction Coeffitlent of Percent reduction from Concentration
(%) 1 2 3 4 5 6 7 8 9 10 (%)" (offspring/female) variation (%) control (%)
36 38 35 32 34 100 34.1 7.5 Not applicable Control 29 35 32 36 34 34 42 34 39 37 100 36.3 7.4 -6.5 10.98% 36 36 33 37 35 39 39 35 32 100 37.1 7.9 -8.8 22% 33 40 40 39 38 36 40 40 38 39 33 36 100 37.9 7.5 -11.1 43.9% 41 37 34 41 37 40 42 45 40 100 40.1 7.2 -17.6 72% 40 38 44 39 36 40 38 39 37' 43 39 100 39.8 5.0 -16.7 100% 42 42 40 38 37 34 40 40 40 34 34 37 37 39 100 37.2 6.8 -9.1 100% Intake Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 2.725 PMSD = Percent Minimum Significant Difference declared statistically PMSD: 8.0 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests significant in a whole effluent toxicity test. On average, a significant Intake: when a toxicant reduces Ceriodaphnia reproduction by 10.8% from the control.
Dunnett's MSD value: 1.932 Lower PMSD bound determined by USEPA (10"h percentile) = 11%.
PMSD: 5.7 Upper PMSD bound determined by USEPA (90'h percentile) = 37%.
in chronic The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerlodaphnlareproduction reference toxicant tests Program. EPA-833-R-O0-003. US Environmental Protection USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Agency, Cincinnati, 011.
Environmental Testing Solutions, LLC Statistical Analyses Ceriodaphala Survtval and Reproductin Test-Reproduction Start Date 1/14103 Test ID" CdFRCR Sample D TVA Sequoyah Nuclear Plant End Date" 1121/03 Lab IDa ETS-Env Testing Solubons Sample Type- DMR-Dsehlarge Monstoong Report Sample Date Protocol EPAF 91-EPA Freshwater Test Species CD-Cerodaphruna dubt, Comments C011c-% 1 2 3 4 5 4 7 8 9 10 D-Control 29000 35000 32.000 36.000 34000 36000 38000 35 000 32.000 34 000 1098 36000 36000 33000 37000 35000 34000 42000 34000 39000 37000 22 33000 40000 40000 39000 38000 36000 39000 39000 35000 32000 439 41000 37000 34000 41000 40000 40000 38000 39000 33000 36000 72 40000 38000 44000 39000 36000 37000 40000 42000 45000 40000 100 42000 42000 40000 38000 40000 38000 39000 37000 43000 39000 Transformn: Untranoformed 1-Talred Isotonic Conc-% Mean N-Mean Mean Min Max CV% N 0-Stat Critical MSD Mean N-Meau D-Contro1 34100 10000 34100 29000 38R00 7.502 10 37550 10000 1098 36300 10645 36.300 31000 42000 7352 10 -1846 2.287 2725 37550 10000 22 37 100 10880 37100 32000 40000 7879 10 -2518 2287 2725 37550 10000 439 37900 11114 37900 33000 41000 7509 10 -3189 2287 2725 37550 10000 72 40100 11760 40100 36000 45000 7194 10 -5036 2287 2725 37.550 10000 100 39800 11672 39800 37000 43000 4997 10 -4784 2287 2725 37-550 10D00 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test iedicatesnormal d&stnbution (p > 0 01) 0826381624 1 035 .0 091994285 -0.52674914 Baudett's Test indicates equal vanances (p 0 90) 1604416966 15 08631706 Itypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetrs Test - 100 >100 1 2724522394 0 079898017 50 71 7098148148 3 4E-05 5.54 Linear Interpolation (80 Resamples)
Point % SD 95% CL Skew IC05 >100 ICIO >100 IC15 >100 10, IC20 >t0o 09 '
1C25 >100 08, 1C40 >100 07 IC50 >100 08:
0o5 05 "I
0) 0 04
'os 01 004 w*...
-02
-031 0 20 40 60 80 100 120 Dose %
Dose-Response Plot 1-tail, 0 05 level oa significance 8
sqno..Ol-14-O3dataxls
Environmental Testing Solutions, LLC Statistical Analyses Ceilodaphnia Survtval and Reproduction Test-Reproduction Start Date 1/14/03 Test ID CdFRCR Sample ID TVA, Sequoyah Nuclear Plant, Intake End Date 1/21/03 Lab ID ETS-Env Testing Solutions Sample Type DMR-Discharge Monitonng Report Sample Date Protocol. EPAF 9I-EPA Freshwter Test Species CD-Cenodaphna dubia Comments Coan-% 1 2 3 4 5 6 7 8 9 10 D-Controi 29000 35000 32000 36 000 34000 36000 38000 35 000 34 000 34000 100 37000 34000 40000 40000 40000 34000 34000 37000 37000 39000 Trantform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-ControL 34300 10000 34300 29000 38000 7148 10 100 37200 10845 37.200 34 000 40000 6801 10 -2.603 1734 1932 Auxiliary Tests StatIic Critical Skew Kurt Shapitro-Wilk's Test minhcates nomnal distnbution (p > 0 01) 0940015912 0868 -0519383606 -0.338943619 F-Test indicates equal variances (p - 0 93) 1 064695001 6 54108572 Hypothesis Test (1-tall. 0 05)
Homoscedastc t Test indicates no sinificant differences sqn._O0-14-O3dataxls
Page 1 of 6 Chronic Whole EffiUent Toxicity Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas Client: -NtA Facility: .w- AA*t,,4eal WeA#,
NPDES#: TM 0CVASO Project #: -571 Dilutionpreparationinformation: Comments:
Dilution prep (%) [0A& 'L 4.9 "_ IGO __Z_
Effluent volume(mL) 16q, -7__ 55_o (____t._5 I_ _o_0 _
Diluent volume (mL) 11- t) &___41__________42_______
Total volume (mL) IS oO (SOO tSm- MOO ISCO _
Test organisminformation: Test information:
Organism age: ZI,-Roo, o t..t Randomizing template: gto f-Le Date and times organisms Or- -, i'oIS-SO Incubator number.
were born between: _-___"__
Organism source: A !,6KrLR Ot-ls-61 Artemia lot number: 6100 GL.
Transfer bowl information: pH = Temperature =- C Total drying time: _'____'___s 2q.2 Date / Time in-ut: ,tAtA Average transfer volume: Date / Time out:
I,.'4 -w- 1L Oven temperature: iv.
Dailyfeeding and renewal information:
Date Morning Afternoon Test initiation, Control water Sample numbers Analyst Day feeding feeding renewal, or batch used used time time termination time 10 I trxTAl 0 ov-i1.(o=b --- 1,550 %4T..0 K \VI.&' G*6uil.* 7.. _,I 2 01-16'-U3 ON~'k IAS S1 - K G-17ZcS3 GSoltbd) OS0116..3ýý 3 01-t0 0%& '.11 HV-& 0;IS03 o__3-i 63o0 0 .o1 o 0 . L 14 6 %&__ _ _ _ _ _ _ _ _ M R S 0 1-11 03sOs w C, eoi1# O %ill 6
Ct- -40*- *"- % _J C- - I ULLC a%-JR-O:L 041.10 . ()% - -9 OXAllf -IQ"
'7 Summary of test endpoints:
Control
%Mortality:information: 670Z cceptance crttena
<520% 7-day LCso =7,{)
- Average weight per initial larvae: o. (,,_a5q NOEC y > IoC, Average weight per surviving larvae: 0.( *394 Ž0.25 mg/larvae LOEC > 100 f ChV IC25 >..... 7r
Environmental Testing Solutions. LLC
- g . U L1 0
I\
Species: Pimephalespromelas Client: S&jkjN -A- %N j Date: o0 0-3 Survival and Growth Data Day CONTROL 10,987.
A B C D E F G H I J K L 0 it) /I0 i0 00 110 0 !0 /0 M0 10 1 10 I01 0 1o 16 l) / (0 10 10 10 JO 2 /1 1)0 /o /C0 to 1 //o /0 I0 10 to 10 3 ( 10 1() 0 t) 10A) JO I0 i010 /0 /6O/
4 to t o t o it) A) A oot to / (j 1(/o )
5IC /0 IA)b 1 /0 16 /0 10 /0 10 [0 t0 6 ID 10 10 to 0 /o 10 /0 t 10 / 16 /0 7 /o lo /0 /C /0 /I0 /0 t0 /0 10 to A~~~a A D# -IkW -I -a 41 -egh -m)(
A - Pan + aveweight (mg) V.0 11.*- ti) le r1ý\ 1 it-q Larvae weight (tug) -A - B I bpA _K; -SO
~ ~ t~1 Calculations and data reviewed Comments:
- "'Environmental Testing* Solutions, LLC Page 3 of 6 Species: Pimephalespromelas Client: *3G4A*" O)t % "Ve#h Date: O\'Nq-'3 t.
S-Survival and Growth Data Day -7. *12-076 l00T.
M N 0 P Q R S T U V W X 0 ID to Io to 10 /o /0 /0 /0 10 10 /1 1 10 lt R 10 tO 10 /0 /0 /0 10 A) 16 /0 2 !0 10 /0 /0 (0 1O
(_
)0 3 10 10 10 A0 10 A) Io o A /()10 t10 4 _ __ 10 tO 10 /0 10 ib 10 10 to 10 /0 IC) 5 It) /0 0 10 /0 0o (0 10 10 t0 16 16 6/0 /0 /0 /0 /0 10 10 /0 i~' 0 10 10 7 1SMALL Isk"L S$IALL - $ C A Pnwihgo 10 /0 (C:) t to ItCo /0 /0 1 0 Ito l0 0
B- Pan + Larvae weight (mg) Ito," 4,Do_ ,,v I 7.7.0p v00I Larvae weight (mg)- A - B1 I
Calculations and data reviewed Comments:
Environmental Testing Solutions, LLC Page 4 of 6 Environmental Testing Solutions, LLC Page 4 of 6 Species: Pimephalespromelas Client: <e-& &NR - O0 JePt Date: ol-jq-63 Survival and Growth Data Day Mnk*P Y- Z AA BB 0 (o0to oI1 1 Jb )O i6 10 2 / 10 10 10 3 A) 6 10/0 A) 4 A) /0 /0(6 5 /b 16 /6 /0 6 (0 /0 16 /0 7 10 Io ID to A - Pan weight (mg) " ___ .
B -Pann+Larvae weight (mg) to r'$'
Larvae weight (mg)- A- B cq% D ~*4.-6x'5 Calculations and data reviewed-Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Test dates: January 14 -21, 2003 Project number. 571 Reveiwed by* fr-/
A m Pan weight i Larva.
B = Pan Larvae weight (mg) Weight I Initial number Mean survival Mean weight (ng) Ceffiident of Pe.r. reduc-tion frm Concentration (%) Replicate Initial number il Final number of larvae (rag) weight (nag) = A- n et larva. (Mn (%) variat() ear (%)
larvae A 10 10 14696 20020 5324 05324 10 15 096 21 960 6 864 06864 1000 06354 13.3 Not applicable Control B 10 C 10 10 14874 22070 7.196 07196 D 10 10 15.059 21090 6031 06031 E 10 10 16204 23.790 7586 0.7586 F 10 10 14686 20950 6264 06264 1000 06795 8.7 -6.9 1098% G 10 10 14980 21870 6.890 06890 1i 10 10 14911 21.350 6439 06439 1 10 10 14847 21.490 6643 06643 J 10 10 14904 21.400 6496 0 6496 1000 06530 1.3 -2.8 K 10 10 14906 21440 6534 06534 L 10 10 15 302 21.750 6448 0.6448 M 10 10 14984 22000 7016 07016 10 10 14982 20740 5.758 05758 1000 06167 106 2.9 43.9% N 0 10 10 14.743 20300 5.557 05557 P 10 10 14643 20980 6337 06337 Q 10 10 14900 22310 7410 0.7410 72% R 10 10 14668 20750 6082 0 6082 1000 06675 8.2 -5.1 S 10 10 14689 21.300 6611 0 6611 T 10 10 14.824 21420 6596 06596 U 10 9 15.112 21350 6.238 06238 100% V 10 10 15.159 22220 7.061 07061 97.5 06587 6.7 -3.7 W 10 10 15.137 22000 6.863 06863 X 10 10 14.725 20910 6.185 06185 Y 10 10 14811 21800 6.989 06989 10 10 15 185 21500 6315 06315 1000 06629 43 -4.3 100% Intake Z 6 518 06518 AA 10 10 14822 21.340 BB 1 10 10 14977 21.670 6693 06693 1 1 1 Outfall 101: MSD = Minimum Significant Difference 00982 PMSD - Percent Minimum Significant Difference Dunnett's MSD value:
PMSD" 155 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Intake Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 17.91/ from the control (determined through Dunnett's MSD value. 00865 Lower PMSD bound determined by USEPA (10th percentile) = 9.4%.
PMSD: 136 Upper PMSD bound determined by USEPA (90th percentile) = 35%
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, Ol
Environmental Testing Solutions, LLC Statistical Analyses Lam! Fish Growt and Survhval Te4-7 Day Growth Start Date 1/14/03 Test a), PpFRCR Sample ID TVA. Sequoyah Nuclear Plant, UV-Trnted End Date 121/013 Lab aD" ETS-Env Tstg Solutions Ssmnplc"ype DMR-Dnsch*ar Monitonng Report Sample Datr Protocol EPAF 91-EPA Freshwater Test Species PP-Punephales pronelas commrents Cme.% 1 2 3 4 D-Control 0.5324 06864 07196 06031 1098 0 7586 06264 06890 06439 22 06643 06496 06534 06448 439 07016 05758 0.5557 06337 72 07410 06082 06611 06596 100 06238 07061 06863 06185 Transfmm Untranmsamed 1-Tailed Isotonic N-Mean Mean M11 Max CV% N t-Stat Crittiol MSD Mean N-Mean CoOC-% Mean 10000 06354 05324 07196 13.275 4 06574 10000 D-Control 06354 10694 06795 06264 07586 8679 4 -1083 2.410 00982 06574 10000 1098 06795 10278 06530 06448 06643 1271 4 -0433 2410 00982 06530 0.9933 22 06530 09706 06167 0.5557 07016 10629 4 0458 2410 00982 06476 09851 439 06167 10505 06675 06082 07410 8216 4 -0788 2410 00982 06476 09851 72 06675 10367 06587 06185 07061 6700 4 -0572 2410 00982 06476 09851 100 06587 Statistic Critical Skew Kurt Auxiliray Tests 0 959238648 0 884 0 156850I23 -0594541763 Shapu o-Wik's Test indicates normal distnbutron (p > 0 01)
Bartlerts Test indcates equal variances (p -0 10) 9132943153 1508631706 NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Frob df Hypothesis Test (1-tail. 0 05) 100 >100 I 0098173695 0154512996 0002049734 0003318839 068807584 5,18 Dunnett'sTest Linear Interpolatioa (80 Resamples)
Paint % SD 95% Ct(Exp) Skew IC05 >100 C100 >100 IC15 >100 100 1C20 >100 09 IC25 >100 08 IC40 >100 07 IC50 >100 06 o 04 S04 S03 02 01 00 - *.. --.
-01
-02 0 20 40 60 80 100 120 Dose %
Dose-Response Plot 1 -tail, 0 05 level of significance sqnOl-14-03dataxis
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and SurvftiW Tet-7 Day Growth Start Date 1114/03 Test ID PpFRCR Sample ID TVA. Sequoyah Nuclear Plant, Intake UV-Treated End Date 1/21/03 Lab ID ETS-Env Testng Solutons Sample Type DMR-Discbhe Momtoong Report Sample Date Protocol EPAF 91-EPA Freshwater Test Speces PP-Pumephales prome.las Comments Coc-% 1 2 3 4 D-ControL 05324 06864 07196 06031 100 06989 06315 06518 06693 Transform Untransformed 1-Talled Cone-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Conlrol 06354 I 0000 06354 0.5324 07196 13.275 4 100 06629 10433 06629 06315 06989 4.308 4 -0 618 1943 00865 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilcs Test indicates normal distnbuhon (p > 0 01) 0976053894 0749 -0.365780657 0 244562257 F-Test indcates equal variances (p - 0 1i) 8725190163 4746834564 Hypothesis Test (1-tall, 0 05)
Homoscedastic tTest incicates no signricant drtlerences sqnO l-14-O3datatxls
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pinmephalespromnelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Mean Temp: 24.8 Test dates: January 14 - 21, 2003 Min: 24.4 Project number: 571 Max: 25.4 Reviewed by:CA,-
Concentration Parameter Da 0 Da 1 Da 2DIa 25.
Maa 1I)a 4_ay Re6wdb:G 5 D)a6 Initial Initial Final Initial Initial lInitial i nal Initial Final Initial F pn (SU) 8.06 8 19 8 15 7 18 809 762 8.16 7.68 8.11 DO(mg/L) 7.9 7.7 7.9 80 78 64 7.7 76 7.6 Conductivity (pmhos/cm) 308 301 303 278 286 28403 Control Alkalinity (mg/L CaC) 64 60 62 Hardness (mg/L CaCtj) 84 8686 Ternperature C 244 25.1 244 25.1 245 247 244 249 244 25.3 246 24.6 24.7 248 pH(SU) 805 802 821 803 8.17 7.72 822 7.61 8 10 758 8 17 770 812 803 10.98% DO (nm/L) 85 80 81 80 7.9 6.9 80 63 76 62 7.5 75 78 7.8 Conductivity (pmhos/cm) 294 298 288 278 286 279 297 Temperature CC) 244 25.0 24.5 25.1 246 24.7 245 249 245 2 246 246 24.8 24.8 H(SU) 807 803 820 804 8.16 7.66 820 761 810 63 817 765 813 808 22% DO (rg/L) 85 80 81 8.1 8.1 6.5 80 63 7.7 67 7.5 7.3 7.8 7.8 Conductivity (pmhos/cm) 288 286 278 266 278 272 286 Temperature CC) 244 25.0 24.5 25.1 246 247 245 249 245 254 246 24.6 248 248 P
1 H (SU) 807 803 819 802 8.17 7.72 8.18 765 809 764 8 15 772 814 809 SDO (mg/L) 86 80 81 8.1 81 69 8.3 63 78 67 75 7.3 80 82 Conductivity (umnihos/cm) 266 263 257 259 254 26 Temperature CC) 24.4 25.0 245 25 1 247 24.7 245 24.9 24.8 25.4 24 6 246 248 24 8 PH (SU) 8.08 803 819 801 8.16 7.74 816 7.69 811 767 8 13 7.72 8.14 8.16 DO (g/L) 8.6 80 81 81 81 68 8.6 66 7.9 65 80 7.5 81 8.1 Conductivity (ptmhos/cm) 235 231 228 220 235 227 240 Temperature CC) 24.4 250 24.5 25.1 248 247 245 249 250 25.4 24.6 246 248 24 8 p11 (SU) 8.07 8.06 8 18 799 8 18 7.79 8.14 7.80 8 12 7.75 8 15 7.88 8.15 8.22 DO (nigL) 851 8.01 8.31 80 831 7.11 86 7.1 8.2 6.8 7.8 78 82 80 Conductivity (limhos/cm) 2001 208 204 213 100% Alkalinity (mg/L CaCO,) 73 71 Hardness (mg/L CaCOQ) 89 8 Total Residual Chlorine (mg 0. <0 10 <0 I0 Temperature CC) 244 25.1 ;4.5 25.1 2481 247 245 24.9 252 25.4 246 246 2468 24 8 pl (SU) 808 806 8 19 804 81 7.79 811 7.72 811 7.72 801 786 814 8.18 DO (mg/L) 8.6 7.9 82 81 82 70 8.8 6.9 -8.51 691 861 801 84 8.3 Conductivity (pmhos/cm) 204 200 200 193 209 202 100% Intake Alkalinity (mg/L CaCq) 70 7274 Hardness (mgIL CaCQj) 8 8282 Total Residual Chlorine (mg <0.10
_Temperature (C) 246 25.1 24.5 25.1 245 247 244 24.9 24.8 25.4 249 246 24248
. Environmental Testing Solutions LLC
.Evione9a Testin Souios LLC 6l~
Species: PimephalesproM elas Client: MRcAR- m Date: _o- pk-3 Daily C'hemis*v Concentration Parameter02
____DO_ Tempegtre) 24.' 4 -4.5 'V (0 4&%Conductivity q (lmhos/cm) ;So
______Temperature (OC) 244z. 4 1 pH(S.U.) .1G.
DO m/)Ll (0 Conductivity Q(rnhos/cm)_ 92
_ _ _ _ Temperature (C) 24A.k 24. 4.! Z4.qO t . z Conductivity
__ _ __ _ Temperature (00) ZA. zsj 24. -Z. tA - k vH (S.U ) a 11 RZ2 S I g. 1 19.01 1 A-,(,o I DO (m /L)
B-11 11 I
-720% 8.1 Conductivity (ptnhos/crn) 0 I 3 flZ31 Temnerature ( C) 7_q
--m eraur (" -1IVV f f N .1I DH (S U.)
A 4 n 1 42.iL DO (mg/L) G, A I ln1r1 Conductivity o
_(pmhos/crn) o zoo Alkalinity (mg CaCO 3IL) 6k~ 13 Hardness (mg CaCO 3/L)
TR chlorine (m(:0.1 Tem erature (0CM 24P pH (S U.) Ia.n 111 - -)L zq. 7SA 6.1-4 1 --2C?
w G1Z! f -- I'. . . .
DO (mg/L)
Conductivity (Iinffioslcm) -zo4 :Zoo Alkalinity l4VrRKe, (mg CaCO3/L) '70 12..
Hardness (mg CaCO3/L) so f2 TR chlorine
/(0. 10 40-10o Temperature (0C)
U .(
Initial niilInitial 11 Final I
.1 Environmiental Testing Solutions. LLC
'aeU 010 Species: Pimephalespromelas Client: CPe'4A M Date: -(xA-0%'O Da Concen- Parameter 3 4 5 6 tratlon _________
CONTROL pH (SU.) 8.18 .i, 6.R5,t,,O9 3o DO m .0 b..6Th Conductivity DO(mgCC3L)
(mg'7 Caondutiit
_______ Temperature (0 C) 2q .5 5. '.. 'V.. ' jJ pH(S.U.) 1,z16 (.
DO (mg/L) P. 4(
jbzMj Conductivity (j'mhos/cm) 24 t
___ ___ Temperature (0 C) 741 zq7~I't
'.s 2 2'l.$0 .'Z4.41, q pH (S U.) 8Z 0 :.t 914S1 DOi(meL G, I. -O S
-221, Conductivity 9
U(
(g+/-mhoskcm)2-&
____Temperature (0 C) zq.f.S ' Vi'4. ML 24.40 zq4 DO (mg/L) 1 (oep 94.0 1
'q5 Conductivity (iimhos/cm)
______ Temperature (0 C) L 4.5 2'S . .L*.o 24.
PHi (S.U.)
I--
.1i4 A. I7) .i Ls ~t I~Z DO (mglL) 6 Conductivity (Amhos/cm)_____
qL Alkalinity (mg CaCO 3/L)
Hardness 7.2O-(mng CaCO 3IL)
Temperature ("C) 7-14 -r!
pH (S.U.) q it DO (mg/L) e -S-5
/0% Conductivity (xnmhos/cm)
/N~7Je, Alkalinity (mg CaCO 3/L) -74 Hardness L(mg CaCO3.fL) 9 2ý TRchlorine(mg/L)I 40.11)
_____ _T~empcerature ("C) 12 q 24-T-Initial 11 Final I Initial 11 Final Fnal Inital II Iitil Fnal I Initial 11 Final
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphniaduibia Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Mean Temp: 24.8 Test dates: January 14 - 21, 2003 Min: 24.3 Project number: 571 Max: 25.6 Reviewed by: Ckrt-Da 0 Day 1 Da 2 Da 3 Da 4 Day 5 Final 1)aInitial 6 Final Concentration Parameter Initial F Initial Final Initial I
IlFinal Final Initial IFinal 806 823 8.19 8 19 8 15 8.21 818 8.18 809 8.16 8.16 8 14 8.11 8 16 pH (SU) 79 8.1 77 8.3 791 82 80 80 7.8 8.1 7.7 82 76 7.8 DO (mg/L) 308 30 0 78 286 2841 303 Conductivity (lpmhos/cm)
Control Alkalinity (mgJL CaCq,) 64 60162 Hardness (mgIL CaCQ) 8 86 248 243 252 246 249 24.3 249 245 253 246 24.8 247 25.1 TemperatureCC) 246 805 823 821 820 8.17 8.22 822 8.19 810 817 8.17 8.16 8.12 8.19 PI (SU) 85 82 81 8.3 79 8.2 80 80 76 81 75 82 78 7.9 10.98% DO (mg/L)
Co1-Cnductivity (limhoslcm) 294 298 288 278 286 279 297 245 25.2 246 249 245 249 246 253 24.6 248 246 250 "TemperatureCC) 246 24.8 8.16 8 13 820 807 824 820 820 8 16 826 8.20 822 810 820 8 17 pH (SU) 85 82 8.1 83 8.1 82 80 80 7.7 82 75 82 78 80 DO m L 22% Conductivity (limhos/cm) 28 286 2780 26 278 272 286 248 24.5 252 246 249 245 249 246 253 247 248 246 250 Temperature*CC) 246 824 819 8.19 817 824 818 8.21 809 8.20 815 8.16 8.14 821 PHt(SU) 807 8.1 82 81 8.2 83 8.1 78 8.3 7.5 8.1 80 80 DO (mgL) 86 43.9% Conductivity (umhos/cm) 266 263 2 246 259 254 64 245 25.2 246 249 247 249 248 254 24.9 248 246 250 Temperature CC) 246 828 8.19 821 8.16 831 8.16 827 811 826 813 821 814 827 PH (SU) 808 83 81 82 81 81 86 8.2 7.9 8.3 80 8.1 81 8.0 DO (mL) 86 72% Conductivit mhoslcm 235 231 228 220 235 227' 240 24.71 252 24.6 24 9 25.0 249 24.9 2593 25.1 248 24.5 250
_Temperature CC) 2461 248 818 8.22 818 8.30 8 14 830 812 8.28 815 8.25 815 8.30 pH (SU) 807 8.30 84 83 8.3 83 8.3 86 82 82 83 78 8.2 82 7.8 DO(mg/L) 85 Conductivity mhoscm0196 208 204 2 100% Alkalinity (mg/L CaC~k) 68 7 71 Hardness (mg/L CaCti) 86 89 89 Total Residual Chlorine (mg[L) <0.10 <0.10<0.10 24.6 24.9 25.1 25 0 24.9 25 3 25 6 24 8 24.4 25 0 Temperature CC) 246 248 2468 25 2 829 819 822 817 827 8.11 827 8 11 823 801 825 8.14 8.30 Pll (SU) 808 8.1 82 80 82 8.1 88 8.2 85 82 86 83 84 8.1 DO(mg/L) 86 204 0 200 19 209 202 214 Conductivity (pmnhos/cm) 100% Intake Alkalinity (mg/L CaCt) 70 727 Ilardness (mg/L CaCOQ) 882 Total Residual Chlorine (mg/L) <0<010 <010 244 25.2 246 24.9 24.8 2501 248 253 249 24.8 245 250 Temperature C) 246 24.8
Page 6 of 7 ny toraimeja rr %10+;" 12 4znl"f;Anq 9LLC Species: Ceriodaphniadubia Date: 01~-14-01 Client: !6pakx4AV;- No 16ý Concentration Parameter02 mDOaCO/L) 3 Hardness
_(m CaCO3IL) zq4., 2q.9 ' '-i~
____ ____ Temperature (T) pH (SU.)
DO (m/)n1 109 1 -~Conductivity 6nhoslcm) 79q (TC) 4( VA 2 S L.Z l.
_________Temperature pH (SU.) gW 2207, Conductivity 2Q ,Z-e Vmhosfcm) A. s.
_________Temperature (C) ZA.t. I ~ . 2.- Z~~?
HOSU.L) e.06 AJ5.9o7 Conductivity Zo Z4 mPhoslcm Temperature (C) Zq.( 16U ZA.
pH (S U).R -A DO(mg)
-72 Co"nductivity z3S B Vmhoslcm) 0 -VA
________ Temperature ( C) 2.C4. (, i.M, &
Conductivity (Vnnhfoslcm)
Alkalinity I Hardness (mg CaCO 3IL)
_______Temperature (0 ) q~, 2x & Y pH(SU.) .o DO (mg/)(
ICO70 Conductivity 2oq 2 l (mhoslcm) 1-z I /F"?Ke, Alkalinity (MgCaCO3IL)
Hardness 10i 0
(mg CaCO 3IL)
Temperature (*C) Zq 4 I IntaFia Initial Fnl Initial Fial I
Environmental Testine Solutions, LLC Page 7 of 7 Environmental Testing Solutions, LLC Page 7 of 7 Species: Ceriodaphniadubia Client: *e,1noo&IA, N TeAx-eAP Date: 01- 14-63 I.
Day I 5 6 Concen tration 4 Parameter t
3 I 4 I I I CONTROL nH (S U. to 1I I 1 I 6.. 1 1 ..1o II II *'*i* I DO (mg/L) 9.0 Conductivity Z80 z0f *3b (igmhascm Alkalinity (mgCaCO 3IL)
Hardness (mg CaCO 3/L d Nt.O Temperature (MC) 14-5~g I i "-" I w -- I- -
I T1 - " - I II vH (S.U.) B ,10 II . I 1z DO (mg/L) 1oP.* Conductivity (Vmhos/cm) I (m 0
I ______-
21A. f-. i-7-S.S 2144 I in's -
Temnerature ( C'l II zS.-o I Temper a..r- (T --- . .
nH (S U.* *. 20 .- 2 7 eR* IIS.
I1 =
I *.A%
I II I1 D* -
oZ I l DO (mg/L) (6.
22% Conductivity Z(o 2ec (g~mhos/cm)_____ ZUz Teminerature (0C* "z'a.'i "zq.h, II II Z-M. I II Z* I DO (mg/L) -. 9T. ;.t5, .1 9.0 .
Conductivity .l 7
'(gimhos/cm) ZW4O 9Z5 pH (SU.) 19.igo 901 13.. 8.1s 8.. A DO (mg/L) Ly3 9)-.
Temperature (C) ".O 2q.& 2
'70o Conductivity -~ ze . c Te(mperature () 2SC - 141 53 L~
(mg CaCOL)
Hardness TR Chlorine (mgfL) *!<0 Qimhos/cm)o Temerature (0() 2,l *$ i q' 2x.3a, , .. V.1. i s.o (mg CaCO3IL)
C--Conductivity Izz
/A)r*qi'e Alkalinity Hardness TR chlorine (mg/L) < 0.10, Temperature (°C) .*..-4 q.S0 II I*ln,,I I nti.l II Final I Initiml II Final I Initial II inal I I aiti l Fi l
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0)
Species: Pintephalesprontelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant UV-Treated Mean Temp: 24.7 Test dates: January 14 - 21, 2003 Min: 24.3 Project number: 571 Max: 25.3 Reviewed by: CL411 Concentration Parameter Day 0 1 tDay Da 2 Day 3 Da 4 Day Da 6 Initial Final Initial Final Initial FInitial I Final Initial Initial Final Initial Final pit (SU) 803 808 8 17 8061 8.14 8 141 776 766 809 7.77 80 801 DO (mgL) 8.1 8.21 861 841 8.3 69 821 741 8.1 69851 78 848.
Conductivity (imhos/cm) 306 309 299128612912921 302 Control Alkalinity (mg/L CaCQ) 64 60 62 Hardness (mg/L CaCQ) 84 866 Temperature CC) 244 24.5 24.3 249 246 247 24.5 250 246 25.1 246 246 24.8 248 pH (SU) 802 807 8.18 802 8.14 7.75 8 12 7.69 810 763 807 778 811 798 10.98% DO (mgL) 82 82 84 8.2 82 69 82 69 8.1 65 86 76 83 7.7 Conductivity (tmhos/cm) 300 296 291 283 295 290 295 TemperatureCC) 245 245 244 24.9 246 247 245 250 24.7 25.1 246 246 248 248 P804 807 818 804 8.13 7.71 8 1 7.71 8.10 766 807 785 8.10 801 DO (mg/L) 80 82 85 82 82 70 82 69 7.9 6.8 86 76 8.3 7.7 22% Conductivity (tmhos/cm) 286 283 278' 273 282 282 280 Temperature C) 245 244 243 249 246 24.7 245 250 247 25.1 246 246 248 248 P804 807 818 804 814 7.74 809 771 8.11 7.66 8.07 783 809 801 43.9% DO (mg/L) 79 82 84 8.3 8.3 69 80 70 78 6.9 8.5 79 82 80 Conductivity (pmhos/cm) 264 260 256 254 261 261 26 Temperature C) 24.5 24.4 24.4 249 246 24 8 24 5 250 24.9 25 1 246 24 6 24 8 248 PH (SU) 8.05 809 818 806 815 7.75 8.08 7.70 811 767 808 782 8 10 801 DO (mg/L) 7.9 82 84 83 83 69 80 69 78 69 85 78 8.2 80 72% Conductivity (pmhos/cm) 230 231 228 226 235 234 233 Temperature(C) 24.5 244 246 249 246 248 245 250 25.1 25.1 246 246 248 248 PII (SU) 804 8.13 818 806 816 7.76 806 8 101 770 807 786 813 805 DO(mg/L) 885 84 83 7.0 7.9 78 69 85 7.7 8.1 8.0 Conductivity (pmhos/cm) 201 20m1991 100% Alkalinity (mg/L CaCQ) 68 7371 Hardness (mg/L CaCQ) 869 89 Total Residual Chlorine (mg/l) <0 1 <0 10 <0810
_______Temperature (C) 24.51 244 246 29 2471 2481 2451 250 2531 2511 2461 246 248 248 PH6(SU) 803 810 816 8 18 782 804 7.76 8.. 7313 805 DO (mg[L) 801 821 84 86 861 711 791 7.1 781 691 84 7.7 83 8.1 Conductivity (pmhos/cm) 19 98 201 1727207 207 100% Intake Alkalinity (mg/L CaCQ) 7 Hardness (mg/L CaCQ)
Total Residual Chlorine (mg<L0 <
_______Temperature(CM 2461 2441 2441 24.91 24.51 2481 2451 250 <2491 2511 24 246 248 24
Environmental Testing Solutions, LLC
-r r, FJ f.,
Species: Pimephalespromelas Client: -gecoxpi AltN Date: 01-A-014 Concentration Parameter02 CONTROL PH (S.U.) 81491 DO migL Conductivity ~ c (mhos/cxnq D(mgCCL) A (mgCondctivity L
___ __ __ Temperature ("C) ?Z4. s4l 2'4. 7-4.(~ q.
DO (mg/L)
L(3 ~ Conductivity (unihos/c) c q.1
________ Temperature (0 C) 2q .4.t 4 7 .t pH (S.U.) 9091el!
DO (mg/L) ý.
12~ Conductivity (jimhos/c) ;?(o 8 2
______ Temperature ("C) N.'j 2A.'.34LA.(
pH (SU.U) ,
DO (mg/L) R
, 7 Conductivity Zl (jirnhos/cm) Z4ZL DO(mgCaO/L) ICogq .
_______ Temperature ("C) 7-qZLI.CVA - (40 pH (S.U) .I R13 DO (mg/L)q Conductivity 70 Alkalinity
______Temperature (TC) Zq zq k Z4 ý AA 1 Z.
p S .3 6In A Ile e.011 e....--h aF 2 Initial Iniia I Final II Initial utq II Vli,;. I
Environmental Testing Solutions, LLC Page 6 of 6 Pae6of6 Species: Pimephalespromelas Client: Seli,%ýoN AA U'J .,! Date: Ohfl4c Concen- Parameter 3 4 5 6 tration CONTROL pH (S.U.) 8.49. 3,,1 3o1 .i DO1 88,G. (mg/9 Conductivity (1xmhos/cm) 7. AZ32 Alkalinity (mg CaC0 3 /L)
Hardness (mg CaCO 3/L) 18
______Temperature (T) L .% 'leOz4L pH (S.U ) Cla1 DO (mg/L) F 40
~ Conductivity
_____Temperature (0 C) . . S LL(
IJ. -24. 0 pH (S.U.) - Q.1 13 1.Cc> (30- 1.10 o 2-201 Conductivity (gnhos/cm) C2 0
_____Temperature ( C) -SO~
1 14.6 z'.. ~s.
pH (S-U.) ~2I Bt ~ l Conductivity
_____Temperature 0
M ) szA. - *A~ .1 zq4.6 -ZI.
At v4F pH (S.U.) 8.1 9-1 ?Vj~ .8o£el Conductivity Z4 (pimhos/cm) Z523 0
______Temnperature ( C) t4. g 2S. SL2A.Q L4 24.~ ?4.Rq pH (S.U.)6.01) 10 804.
DO (mg/L) - 5.,
Conductivity e
,~7(prho MeZ/cm)IZ0 Alkalinity (mug CaCO 3/L)
Hardness (mug CaCO3fL) t TR Chlorie(L .L.0-10
______Temperature (T) 29.S.3O 251 z A. .z &
PH (S.U.) .rM II A. 17- iii-W DO (mgIL) 4-0 Conductivity le.% (prnhos/m 70 -it Alkalinity (mg CaCO3IL)
Hardness (mug CaC 3 L TRchlorine(gL Temperature (0CM t --
Initial 11
Environmental Testing Solutions, LLC Page 7 of z Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3 /L Analyst JLIIE d Date analyzed DOi'Zj-Tirapt normalit4andmultiplier determination:
Titrant Normality- End Total Normality (N) of EDTA pH Factor or Multiplier reference standard nil mlC = 0.2/E = (N x 50000)/50 ml sample number number (E) ac e 0.018 - 0.022) = Nx 1000 Laborator, control standard:
Reference True value Sample Hardness (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCOI.L) (ml) mnl ml nl 90 to 110%)
t - . 40 50 le. 2 z Z.- '42I.V2. 103, ,
Duplicatesam le precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) {(S- D) I[(S+D)/21) x 100 number (ml) ml ml ml (acceptable range - *-10%)
1,_OT_-CG I 6D zI.C, 255" ,3A, Zo. (4 0 00)*.
Duplicate 5(P z,- . 4 ~zoD S2..
o 2.5D S - s.-.5- 4-0 20 Samnie measuremnents Sample volume Begin End Total- Hardness Sample number Sample ID (ml) ml ml ml Multiplier (Mg CaCO,/L)
Blank 6 "= t-,.o _ ,*, _ 1 or_ ,"0 ; -
-1.S 4,o- -I 0 0M. 0"2- J. -. 57-) 35.5 SQ5 .o 632 p*02Ll.2 EA S 25 z 26-.6 3.1 24) LO4C.
Noe >Sample oeasuremntis:uesml utb iue.Rviwdb:Z ~ Dt eiwdQSC Note: If >1 5ml oftiftant is used, sample must be diluted. Reviewed by: FL66-y- Date reviewed I (" ZOO I
Environmental Testing Solutions, LLC Page 14 '
Page I of JI Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Analy st 1 4t 9. 1 1 Iodide reagent: J CO-0 Date analyzed [ 1,4-0 Acid reagent:
Slope: -q'. II]
Calibration:
Note: Fs e0.10 mg/L 1.00murl Reference standard number I I jMlfE II Note: For samples with a residual chlorine of > 1 0 rngf, the calibration range must be adjusted to bracket the chlorine levels of the samples Laboratorvcontrol standard:
Reference standard True value (TV) Measured value (MV) % RS = MV I TV x 100 number (mgL) (mg/L) (acceptable range = 90 to 110%)
I WtsO) / 0.50 o.Z7\ *o2.2%
Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)121) x 100 number (mg/L) (acceptable range -
- 10%)
ab%14. - stwj lot cLe.APR.N s'owCO i' <0. 0 06Z1 Duplicate D.(. 2 0 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number
!iiiliiiiiiiiiiiiiiiiiiiBlank (mg/L)
(should be =< 0 10 m/)iiii~ii i i:iiiiiiiiii*~****~ ¢ .O**I
......... ~........... Bak(hudeO m ). ............ ...... Z..........111-. 00 1ýo)t14. 2 -. IG Mx1'l tp.-. C.e~AR N0 Cotoz < 0.0o0lS7Z
__:_O\ _t_._q 6 ,"1'Zf-'fL.L t4o CoLouP &.A(K PA*'tLeS < 0.,o000( 2-1 ooi l4.c63 G."ro,.. G .LU Auj AO0 aLc e 6LAC.< 1OAer'tC.lJ C). C6 3'-LL Note: All samples were analyzed in excess of EPA recommended holding tune (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)
IA oq 0.50 0 - et(
o.,-/ q9P.2%
Reviewed by c Date reviewed "O3
Environmental Testing Solutions, LLC "Page___
Page I of I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Analyst K-,p Iodide reagent: I W 0,o.(
Date analyzed U - .D Acid reagent: I MAon:
Slope: 50 . B) -
Calibration:
0.10 mg/L 1.00Img/L Reference standard number I N.=*; I Note- For samples vith a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laborator control standard:
Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range - 90 to 110%) I I &ý ý 1o0.50 5 Z).60 6 1n u *240 Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine
-- number (mg/L)
.****....,...,,.8..m tcb<Olmg ...
- 5 0l11. 02- G Q,. Ir,ST" 0 606f-,l¢.. N109,Jio wg,' . .o:q.
0n0-Note All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted Laborator' controlstandard:
Reference standard True value (TV) Measured value (MV) % RS = MV/ TV x 100 number (mg/L) (mg/L) (acceptable range - 90 to 110%)
0.50 o. 5"Oz oo .q 10 Reviewed by Date reviewed j
Environmental Testing Solutions, LLC
. 1 .1 Page 6 Page I of I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Analyste" Date analyzed .I -0 Iodide reagent: [ i NIyc-b Acid reagent: 1% R05L Slope: " 1B.0 ?,
Calibration:
................................. i of 00.1./ L, Reference standard number m g 1.00t W = Ii Note: For samples with a residual chlorine- of > 1.-0 Mgf1, the calibration range mus bea stdt bracket the chlorine levels of the samples.
Laboratorycontrolstandard:
Reference standard P True value (TV) Measured value (MV) % RS = MV/ TVx 100 number (mVPL) (mg/L) (acceptable range - 90 to 110%)
0.50 o. 53q.
Laborator controlstandard:
eference f standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mgfL)
E0.50 0-5-33 (acceptable range - 90 to 110%)
1NO Reviewed by Date reviewed L~ t.co--j
Environmental Testing Solutions, LLC Page I Of Z..
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst i I]
Date analyzed C)1-Q.- Titrate samples to pH = 4.50 S.U.
Tirantnormality and multiplier determination:
pH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2 CO3 x 0.05)/E (Nx 50000)1100 ml sample water reference standard ml ml ml = 0.25/E -=Nx 500
= 4.5 S.U. n e, number (E) (acceptable range - 0.018 - 0.022)
,..X,
,b
_ lt, _ aOr,
_ 1l*SLO oZt IZ.S" 12". D.02 - 1.0.0 0.0- O .%,.n Laboratory control standard:
Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (rag CaCO/L) (acceptable range (ing CaCO,/L) (ml) ml ml ml -90to110%)
100 lOO 12.4 22- 104 10.1 1(0.Z toz Duplicatesple precision:
Sample Alkalinity %RPD =
Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) {(S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range -h -10%)
OI 1846A mIi S 10 0 _2.5 ___0 _0_t ____(_
DI-0.:W IDuplicate Z8.P 535.0 (D- I ID to.
Matrixspike recover : V e._)g
! 4, J.tL*,
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mag CaCOsIL)
(mg CaCO-IL) 50 6L) (ml) 100 ml Z*8-
- ml
.1o. 11.ml ID.t 12_0 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (Ing CaCO3IL) MV = A - B (acceptable range (MRgCaCOL) = 75 to 125%)
LOS I or)
Sample-measurements:6 Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (me CaCOa/L) n 1. 18 .0-38 0__5 (O.6 6,C __ _
4U 01- ZZ -03 5__ v- t~ (0 0Y-15-o1 *.0.8 i U,3.3 (0o 01 -to 150Su)$ RzO _J.L 224 3._ .-.
O34-N.-03 4 1-ov1-n GCo& A'-- _z2.SL Z's .o 0oo0114.0I et I~t1 I MZ.,4 52.1 3 0
o;o, io.ol __,__
32,1 JQ. 3 --7 32,q %4 T.O _ _
37
____toe)
Reviewed by: Datet reviewed:
r.e-. 1 00 1 03 1
Environmental Testing Solutions, LLC Page 7- of l.
Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L).V-I Date analyzed p.IDý Titrate samples to pH = 4.50 S.U.
Laboratorycontrolstandard; Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (rag CaCO 3 1L) (acceptable range (mg CaCO3/L) (ml) ml ml ml - 90 to 110%)
bSlOl 100 100 . C0..j [IA. co 1(X)
R'fn#rh .cnikp rnvrv Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3IL)
(mg CaCO 1/L) (ml) ml ml ml IN sibI 6b I[o ni.z Ai.3 m21 104 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (tg CaCOIL) MV = A - B (acceptable range (mag CaCO1IL) - 75 to 125%)
enok re o L MatriSapemeasurements: t,. oo 4to io,,i't Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml I ml Multiplier (ml! CaCO3 IL) 0__.4,_ 0 i1.,'9 1 100 l...o . -__ 10.1 10 05o01 OBZ 412 ,4.-3 - -44 t F=,io Fp 2-.
Ze fDPrP F-te R(i0e b: 2s r1)iewed:
SZOaik Vk q.0-14 Reviewed by: I REI Date reviewed: I C4 -Z 8-03 1
Total Hardness (EPA Method 130.2)
Analyst[l*. Matrix: Water, MDL = 1.0 mg CaCO 3/L Date analyzed Titrantnormali and multiplier determination:
Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml 00.2/E - (NX 50000)Y50 ml sample number number (E) (acceptable range - 0.018 - 0.022) -Nx 1000 1NCt~) I~C3Z. ' L 4.- O.VZC -P 20 AP Laboratory control standard:
Reference True value Sample Hardness (MV) %RS=MV/TVx 100 standard number (TV) volume Begin End Total Multiplier (MngCaCO3/L) (acceptable range (mag CaCO3/L) (ml) ml ml ml - 90 to 110%)
I ý5610 I 40 50 l04 17.1 2,L-> Zot.,, '417,-t. I>,2 Duplicatesample precision:
Sample Hardness %RPD =
Sample Sample ID volume Begin End Total Multiplier (mag CaCOsL) ((S - D) I[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range - b 10%)
01, V5.03 M k144zo 'j.2-Ol~ll:* mp-, 5/ I 1_0z.-1 IF -.. -~ r ZO0.o D I Matrix spike recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (rag CaCOs/L)
(mg CaCO1 IL) (ml) ml ml ml 40 *,,' 23,0 V, 20-(0.e6 Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOs/L) MV = A - B (acceptable range (mag CaCO 3/L) 75 to 125%)
Sample measurements:
Sample volume Begin End Total- Hardness Sample number
""su 01,10-03 A Blank M I+s Sample ID be - 0o-mgcaCO3.L) 147-0 (ml) 5"0 ml 1.0 ml 1.0 ml 0.0 Multiplier 20.4 2 ( 1 (mg CaCO3 /L) 03.leC3t3 ,2 1.12e 2Z.z Z0c .Z*{, .31. 3.cqA ___ -
6S
- 0) .Z5 3 ,3.
iOOZ, ,HZ AL, Ul1.5 2.Z - 11 oso) ILI 05 O -'s.D" Ikq-L3'( 2.1 q3 o0 ol. o'1 t tI t t,.8 1-.0 4.2 5(0 vol 16 -0 1 z ___ (.-o 6-3 L4.3 9
_____it___ 3 jK* cOU I~ q__5 I813 Note If >15ml of titrnt is used, sample must be diluted. Rcvewcd by: I Rý Date reviewedl C)I.*e-°01ý I
Sequoyah Nuclear Plant Biomonitoring January 14 - 21, 2003 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales pronelas &*
using Moderately Hard Synthetic Water Test number Test date 7-day IC2 s CT S Control Limits SAiso Warning Linits Control Limits CV (g KCI/L) (g/L KCI) CT-2S CT+2S CT-SAAo CT+SA.t0 CT- SA.2s CT+SAs I 05-07-02 061 2 05-23-02 0.76 0 69 0.11 047 0 90 008 0.57 081 0.14 048 0.90 0.15 3 05-24-02 0 66 0 68 008 0.52 0 83 008 0 56 0.80 0.14 047 089 0.11 4 06-04-02 0 67 0 68 0 06 0.55 0 80 008 0 56 0 80 0.14 0.47 089 0 09 5 06-11-02 0 70 068 0 06 0.57 0.79 008 0.56 0 80 0.14 0.47 0.89 0.08 6 08-06-02 0.55 0.66 007 052 0 80 008 0.54 0.78 0 14 045 0 87 0.11 7 08-13-02 058 0 65 007 0.50 0 79 008 0 53 0.77 0.14 0.44 086 0.11 8 08-20-02 0 63 0 65 007 0.51 0.78 008 0 53 0.77 0 14 0.44 0.86 0.10 9 09-10-02 061 064 006 0.51 0.77 008 0 52 076 013 0.43 0 85 0.10 10 09-17-02 064 064 006 0 52 0.76 008 0 52 0.76 0.13 043 0 85 009 11 10-01-02 0.60 0 64 006 0 52 0.75 008 0.52 0.76 0.13 043 0 85 009 12 10-01-02 047 0 62 007 048 0.77 007 0.50 0.74 0.13 0,41 0 83 0.12 13 10-08-02 053 0 62 008 047 0.77 007 0 50 0.74 0.13 0.41 0.83 0 12 14 10-15-02 0 66 0 62 0.07 0,47 0.77 007 0 50 0.74 0.13 0.41 0 83 012 15 10-22-02 064 0 62 007 048 0.76 007 0.50 0.74 0.13 0.41 083 0.11 16 11-05-02 0 59 0 62 007 048 0.76 007 0 50 0.74 013 0.41 0.83 011 17 12-03-02 061 0 62 007 0.48 075 007 0 50 074 0 13 041 0.83 0.11 18 12-03-02 0.52 061 007 0 47 0.75 007 049 0.73 013 0.40 0 82 0.11 19 01-07-03 0 64 061 007 0 48 0.75 007 0.49 0.73 0.13 040 0 82 0.11 20 01-14-03 0.61 061 007 048 0 74 007 049 0.73 0.13 0.40 0 82 0.11 Note" 7-d IC2 s = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT - Central tendency (mean IC25)
S = Standard deviation of the IC25 values S,,, = Standard deviation corresponding to the 10h percentile CV. S,10 = 0.12, as determined by USEPA for the method and endpoint.
SA.25 Standard deviation corresponding to the 2 5h percentile CV. SX2s = 0 21, as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values percentile CVs. These ranges are more stringent than the control and warning limits Control and warning limits were estabtished using the standard deviation of the IC2, values corresponding to the 10h and 2 51h recommended by USEPA for the test method and endpoint USEPA Recommended Control and Wamia Imnits.
Warning Limt = Standard dewation corresponding to the 75h percentile CV. SAM - 0 38.
Control Limit = Standard deviation corresponding to the 906' percentile CV. SA.wo = 0 45.
USEPA 2000 Understanding and Accounting for Method Variabitity in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-933-R-00-003. US Environmental Protection Agency, Cincinnati, O I Organtsmsobtainedfrom Aquatic BioSystems, Inc 01-14-03 Ads
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 i aI I I C I mI 1 I I I I I IC I I 1.1 Warning and Control Limits Set According to 1 0 th and 25"'Pecntl CVs.
10 0.9 0.8 07 In r-- 0.6 0.5 0.4 0.3 I I I I I I I I I I I I I I i iI i l Test date 7-day ICs2 5 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC 2 5)
- -- Warning Limits (mean IC25 + SA.l 0 , SA.10 = 0.12, as determined by USEPA for the method and endpoint)
. Control Limits (mean IC2 5+ S A25, SA25 = 0.21, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 I I I I Ii m I AI I I I I I I I I 1.1 F Control Limits Set According to +/--2 StandardDeviations.
1.0 -
-- 0.9 o0.8 0.7
- 0.6 t- 0.5 0.4 1-0.3 I
, I I I I t I I I I I I 1I I 1 Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC 25)
Control Limits (mean IC 25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Control Sural Control Mean Test number Test date Survival CV CT MSD PMSD CT Growth (06) for Control
(%) (mg/larvae) (%) for PMSD (%)
Growth CV (%)
I 05-07-02 97.5 0.458 12.3 0 07 15.4 2 05-23-02 100 0 378 9.9 11.1 009 22.5 19.0 3 05-24-02 97.5 0.444 21.3 14.5 0.11 25.0 21.0 4 06-04-02 100 0.682 140 14.4 0.15 22.1 21.3 5 06-11-02 100 0 650 3.7 12.2 0.11 16.6 203 6 08-06-02 97.5 0694 22.2 13.9 0.16 23.5 20 9 7 08-13-02 100 0 659 13.6 13.8 012 17.5 204 8 08-20-02 100 0 765 8.5 13.2 012 16.3 199 9 09-10-02 100 0 854 1.2 11.8 011 12.5 19.1 10 09-17-02 100 0.824 13.4 12.0 0.12 146 186 11 10-01-02 97.5 0.750 18.4 12.6 019 25 4 19.2 12 10-01-02 100 0.975 127 12.6 013 12.8 187 13 10-08-02 97.5 0 929 8.0 12.2 018 19.5 188 14 10-15-02 100 1.037 169 12.6 023 21.9 190 15 10-22-02 100 0 822 106 12.4 0.13 156 187 16 11-05-02 100 0 874 28 11.8 0.12 13.8 184 17 12-03-02 100 0852 9.1 11.7 0.12 13.7 182 18 12-03-02 100 0 668 104 11.6 0.15 22 4 184 19 01-07-03 100 0 886 41 11.2 0.14 15.7 183 20 01-14-03 100 0 677 30 108 0.07 110 179 Note- Average control growth (mg/larvae) = 0.744 CV = Coefficient of variation for control survival On average, the CV for control growth is 10.8% in Environmental Testing Solutions, LLC Pimephales chronic toxicity tests Lower CV bound determined by USEPA (101h percentile) = 3 5%
Upper CV bound determined by US EPA ( 9 0n percentile) = 20%
MSD Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 17.9% from the control Lower PMSD bound determined by USEPA (I0e percentile) =94%
Upper PMSD bound determined by USEPA (90th percentile) = 35%
CT = Central Tendancy (mean Control Growth CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-833-R-0O0-003 US Environmental Protection Agency, Cincinnati, OH.
Organisms obtainedfrom Aquatic BjoSystems. Inc 01-14-03.xls
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water I I I I I I I I I I I I I I I I I I I 40 1-30 [
,-IN
-20 10 F-0 I I I I I I l___
I I I I I I I II I 1 I 1 I 1 1I 1 1I 1 1I 1I 1I 1I 1 Test date
-- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant S- -Central Tendency (mean PMSD)
........ Lower and Upper PMSD Bounds Lower PMSD Bound (10th percentile) = 9 4%, Upper PMSD Bound (9et percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Page 1 of 5 Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number: _
I Dailyfeeding andrenewal information:
Date Morning Afternoon I Day Test initiation, Control water Analyst feeding feeding renewal, or batch used time time termination time 0
0lI'403 I 15:50 iqHAS 0~1-112-03 2
ILISr 3 Isil BII H14S I5 2O 4 0-.-
565 mds-7 I 8 #4&5 OiS-O I 5
152_0 ml3 )I-a .05t' y 6
7 IS cc)
I Environmental Testing Solutions, LLC Page 2 of 5 PpKCICR Test Number: 5 Survival and Growth Data Day CONTROL 300 m KCI/L 450 mg KCI/L A B C D -E F G H I J K L 0 )C 10 /I /D /0 /D /0 /o /0 /0 /0 /0 1 /0 (0 10 (() Ic) (0 10 lo) 116 i0 to /01 2 to to 1O/0 /o to / !/0 /6 q /o' !0 3 I0 10 10 /0 1I0 10 I0L) /o Iz' 'R(
3 J, I/1 io o 'oic / !o0I0 9* 'f I3 L 4 /6i 16 0 10 1
/o/00 to c to 5 10 1o I)0 10 10 10 /I ) 10 '* q to 6 /0 10 10 10 1c J 1/0 10 1 C'
/0 $0 /6 /0 /10 to 'i B - Pan+Larvaeweight(mg) ,t0b 'L A . . '4 A, ' ,
Larvaeweight(mg)-A- B k %0.L t, xw, 1 10S t. 3,.,
- s 4. . 0 3A Calculations and data reviewed- A_
Comments:
I Environmental Testing Solutions, LLC Pa*e 3 of 5 Paue 3 of 5 PpKCICR Test Number. 3 Calculations and data reviewed.._
Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPAI600I4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number. PpKCICR # 43 Test dates: January 14 - 21, 2003 Reveiwved by-_________________
Cncenlration (.Z/L Replicate inlial number of Final number of A - Pan weight B - Pan
- larvae Larvae weight (mg) Weight I Initial number Mean survivnl Mean weight (mg) Coefficient of Percent reduction from KC) larvae larvae (mu) weight (mg) - A- B of larvae (mn) (%) lariaton (%) control (%)
A 10 10 15051 21 720 6669 06669 Control B 10 10 15 100 21990 6 890 06890 1000 06772 30 Not applicable C 10 10 14935 21920 6985 06985 D 10 10 14985 21 530 6545 06545 E 10 10 14592 21 140 6548 06548 300 F 10 10 15060 21460 6400 06400 1000 06287 3.7 7.2 G 10 10 14658 20690 6032 06032 I1 10 10 14733 20900 6167 06167 1 10 9 15001 20210 5209 05209 450 J 10 9 15006 21 730 6724 06724 925 06137 109 94 K 10 10 15000 21 510 6510 06510 L 10 9 14646 20750 6104 06104 N1 10 8 14763 19720 4957 04957 600 N 10 10 15 114 20340 5226 05226 875 05205 10.7 23.1 0 10 9 15 184 21 150 5966 05966 P 10 8 15219 19890 4671 04671 Q 10 6 15087 18240 3153 03153 750 R 10 7 15125 18320 3195 03195 575 02806 27.1 586 S 10 3 14685 16350 1665 01665 T 10 7 14709 17920 3211 03211 U 10 2 14979 15880 0901 00901 900 V 10 2 15172 16000 0828 00828 225 0.1111 726 836 W 10 1 14996 15430 0434 0 0434 I X 10 4 15120 17400 2280 02280 Dunnett's MSD value. 00747 MSD = Minimum Significant Difference PMSD 11.0 PMSD = Percent Mmnimum Significant Difference PMSD is a ireasure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On aerage. a significant difference occurs for Environmental Testing Solutions.
9 LLC chronic toxicity tests when a toxicant reduces Pimcphales growth by 17 1/ from the control (determined through reference toxicant testing)
Lower PMSD bound deternined by USEPA (10th percentile) = 9 4%/,
Upper PMSD bound deternined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Prniephales growth in chronic reference toxicant tests USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA.833-R-00-003 US Environmental Protection Agency, Cincinnati. OIL Organismsobtainedfrom Aquatic BioSystems. Inc 0 1.14-03 Als
Iý Environmental Testing Solutions, LLC Statistical Analyses Larval risk Growth and Suroival Test-7 Day Growth Start Date 1/14/03 Tedt 1a PpKCICR Sample ID REF-RefToxicant End Data 1121/03 Lab ID" ETS-Env Teo"sg olutiona SampleType CL-Potmasum chlonde Sample Date Protocol EPAF 91-EPA Fraahwatee Test Species- PP-Pueephales prooselas coenmento Conc-mgf/l 1 2 3 4 D.-Contzol 06669 06890 06985 06545 300 0.6348 06400 06034 06167 450 0.5209 06724 06510 06104 600 0.4957 0.5226 0 5966 04671 750 03153 0.3195 01665 03211 900 00901 00828 00434 02280 Transform Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CVV. N "-Stat Critical MSD Mean N-Mean D-Control 0 6772 10000 06772 06545 06985 2.971 4 06772 1 0O00 300 06287 09284 06287 06034 06548 3664 4 1487 2290 00747 06287 09284 450 06137 09062 06137 05209 06724 10915 4 1949 2290 00747 06137 09062
-600 05205 07686 05205 04671 05966 10675 4 4805 2290 00747 05205 07686 750 02806 04143 02806 01665 03211 27 123 4 0 2806 04143 900 01111 0 1640 01111 00434 02280 72567 4 01111 01640 Auilliary Tests Statistic Critical Skew Kurt Shapuo-Wk's Test mdicates normal datcsbuton (p > 0 01) 0978783071 0844 -0 294340189 0 81047745 Bartlett's Test indicates equal variances (p - 0 17) 508170414 11 34488201 Hypothesis Test (W-tail.0 05) NOEC LOEC ChV TU MSDu MSDp MSB MASE F-Prob df DunnettaTest 450 600 5196152423 0074686143 0110282614 0017191687 0002127351 0003268048 3,12 Linear Interpolation (80 Resamples)
Point mafL SD 95% CL(Exp) Skew IC05 20947 9935 10051 61549 09262 ICd0 45672 6867 17835 53306 -08708 ICI5 51123 3688 38526 64637 -00122 10 IC20 565 74 28.33 483 20 644 00 02266 09 1C25 60787 Isis 53808 64429 -03037 1C40 671.38 12.57 63430 70760 02316 08 IC50 713 73 1639 66692 75768 01007 07 "idicates IC entumate less than le lowest concentration -06 ,
LC005 0.
S04 03 02 01 00 0 200 400 600 8oo 1000 Dose mgIL Dose-Response Plot 08, 07 1-tail, 0 05 level of significance 05 004 003 02 01, 0
6 Organismsobtainedfrom Aquatic BioSystems, Inc 01-14-03 xls
e J Environmental Testing Solutions, LLC Statistical Analyses Larval 8hh Growth and Survival Teol.7 Day Survival Start Date 1/14/03 Tot LD PpKCICR Sample LD REF-RefToxacaLt End Date 1/21/03 Lab M" ETS-Rav Toe*n Soluion Sm'ple Type KCL-Potamum chlonde Sample Date Protocol. EPAF9l-EPAFrwhwster Tee Spume. PP-Punephales promelbu Comments Conc-imngL 1 2 3 4 D-Control 1 0000 1 0000 I 0000 I0000 300 10000 10000 10000 10000 450 09000 09000 10000 09000 600 0.800 10000 09000 08000 750 06000 07000 03000 07000 900 02000 02000 01000 04000 Transform- Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mena Mean Mi. Man CV% N Sue Critical Resp Number D-Control 10000 10000 14120 14120 14120 0 000 4 0 40 300 10000 10000 14120 14120 14120 0 300 4 1800 1000 0 40 450 09250 09250 12898 12490 14120 6318 4 1200 1000 3 40 600 08750 08750 12188 11071 14120 11.906 4 12 00 1000 5 40 "750 05750 05750 08620 05796 09912 22.581 4 1000 1000 17 40 "900 02250 02250 04834 0.3218 06847 31014 4 1000 1000 31 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wlkx Test mdrcates normal dau'tbution (p > 0 01) 091671133 0884 -0316165842 1.346744523 Equahly orvaannoce cannot be confirmed Hypothesis Test (2-tail. 0 05) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670 8203932 Maximum Likehhood-Problt Parameter Value SE 95% Fiducial Limits Control Chl-Sq Critical P-value Mu Sigma Iter Slope 7973053748 1 226780987 5 568562967 1037754453 0 4 775674923 7814724922 0 19 2 884339205 0 125422458 3 Intercept *1799699151 3501004343 -24 85896016 -11 13502287 TSCR 10 Point Probits mg/L 95% Fiducial Limits 0to ECUI 2674 391.3478345 298 8202155 454 7289305 08 EC05 3 355 4764722581 3940635725 5316781157 08 ECIO 3718 529 180533 455 680513 579 1682188 07 ECI5 3964 567 9984976 501707135 6146857919 T EC20 4158 6008706682 5406378554 6455854668 a06 EC25 4.326 6305841393 5753650197 6745827642 005 EC40 4747 7121373932 6649097825 7628281132 04 ECSO 5000 7661948124 7175883266 8302770165 EC60 5253 8243556709 7689792198 910 1076376 03 EC75 5674 930969317 8545525702 1070235307 02 EC80 5842 9770064101 889455908 1143 436754 EC85 6036 1033549401 9312294474 1236051701 01 EC90 6282 1109365193 9857885374 1364419056 00 1 ý. .. . .
EC95 6645 1232085304 1071 437863 1581.301415 1 10 100 1000 10000 EC99 7326 1500083795 1250 293331 2089403779 Dose mgPL Dose.Response Plot Organisms obtainedfrom AquaticBtoSystems, Inc 01-14-03 xls
SEnvironmental Testing Solutions, LLC Page 4 of 5 Environmental Testing Solutions, LLC Page 4 of 5 PpKCICR Test Number: 5 Daily Chemistry:
I Day Concentration Parameter 0i 1 2 CONTROL pH (S0.) 8_,__p 6.1" _
DO (m_£ /L_*__4_ q -719 .
Conductivity
(_Wnhos/cm) 1306 11;0.1 ,,
Alkalinity (mn CaCO 3IL) (00 Hardness (mg CaCO 3/L)
~(oc) Temperature 24, z.2 - 4. 'Aq--_*qL2."
pH (S.U.) 5.05 11(.oa .,.
DO(mg/L) ") 71. ( 4. e E3, (P 300 mg KCI'L Conductivity (gmos/cm)
Temperature cC) z-4.S *4-. 2A.3 zI..L "Lq.1 -,.7 pH (S.U.) 6., 19, 1E DO (mgL SA 450 mg KCI/L Conductivity (gmhos/cm) Ii 2 J1 zq Temperature (0c) *LI.
, ! '2s 7.qqt243 qs-3 24.I
- l PH (S U.) e, w4 Igo 8.1.D 6.13 3C3 DO (mg1) .-0 -9 .4 6 600 mg KCIIL Conductivity (mhos/ci) 4O2. ",
Temperature Z4 . L3 2q ,2 - ..
(cC)
PH (SU.) ,8,0B -4.qB3 .14 6.) I8.1n .,
DO (mg/L) &Z I g-) qe .4 .o ,
750 mg KCIIL Conductivity (gmhos/cm) (t I** iri Temperature q Z z q (nc) Final i F-inal -niti. Fi.a 900 mg KCI/L Conductivity 1 G13 Temperature zq...Z 'Z", 7qq .7 (0c) q* , *,7. 4.
I Initial II Final IInitial Final IInitial I Final
Environmental Testing Solutions. TTC lr%_ ___ a' *'
E S........
n o rage 0of 4t PpKCICR Test Number: ..2 Da y Concentration Parameter 3 4 5 6 CONTROL pH (S.U.) 1I 8.0q S I.t "0 DO (mgL)
Conductivity (gnmho./cm) z 72MZ, ZS[
Alkalinity (mg CaCO 3/L)
Hardness (mg CaCO3IL) .8(0 Temperature (oC) 2q.4.* - 2 .. (O 1 . zqk" DO (mg/L) 01.3 B,.q 9*7_
.(0 Z)).q -4.9*
300 m g K CIIL Conductivity pH ( 4 8 I l 1
(_mhos/cm)C) - 4 4 A Temperature [
60cO.) CL/.D _iK.q..). ,.A.
pH (S.U.) I e.15 -431.1 :t 40 "4.90 450 mg KClL Conductivity (gmhos/cm) Io33 ItO4 l~
pH (S.U.) -;.t-4S Ls. 15 IS 600 mg KCI/L DO Conductivity O (mg/L)
(mgI 6,5r5,3 4- 0(
15."0 (gmhos/cm) 14I
_____cC) Te m pe ra tu re -*.
I9. zq- .4 Zq.*,
- .p zq.q, 2 . "q.(., -.q.q zq.7 q
750 mg KCI/ Conductivity Temperature 2
_0 pH c )(S.U) Rl O
1"1,.00 4z
-3 z L1.. 5 zq .j,, i.q -.5S.
"1,3t 0.1 6.!5 . -ý -'-"3, q
DO (ma/) "G5f, ]". C,1 .q 4--*3 -=.
900 mg KCIIL Conductivity (gmhos/cm, too'*e) 1 1-* 192q Temperature c)2q,q 2 .Sz*. zq.* zq.5 "2q,G, -e.q zq/.
Initial Final Initial Final Initial Final Initial 1 Final I bqi3co 6 14 -4M
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water Test number Test date 7-d IC25 CT S Control Limits SA-10 Warning Limits SAss Control Limits CV (g/L NaCI) (g/L NaCl) CT-2S CT+2S CT-SA.10 CT+SA.io CT - S.2s CT + SkIS 1 11-01-01 1.00 2 12-05-01 1.05 1.03 004 096 1 10 0 08 095 111 0.17 0 86 1 20 004 3 01-09-02 0.91 0.99 007 0 84 113 0 08 091 1 07 0.17 0 82 1.16 0 07 4 02-05-02 1.07 1.01 007 086 1.16 008 093 1 09 017 0 84 1.18 007 5 03-19-02 1.03 1.01 006 0 89 1.14 0 08 0.93 1 09 0.17 0 84 1.18 006 6 04-09-02 1.03 1.02 0.06 0.90 1.13 0 08 0.94 1.10 017 085 1.19 006 7 05-07-02 1 05 1.02 005 0.91 1.13 008 0.94 1.10 017 0 85 1.19 005 8 06-04-02 1 06 1 03 005 0 92 1.13 0 08 095 !.11 0.17 0 86 1.20 005 9 07-09-02 1.03 1.03 005 0 93 1.12 0.08 095 1.11 017 0 86 1 20 0 05 10 08-06-02 1 05 1.03 0.05 094 1.12 008 095 1.11 0.18 0 86 1.20 0 05 11 09-04-02 1 05 1.03 004 0.94 1.12 0 08 095 1.11 0.18 0 86 1.20 004 12 10-08-02 1.03 1 03 004 0 95 1.12 0 08 095 1.11 0.18 0 86 1 20 0 04 13 11-05-02 1.03 1.03 004 095 1.11 0.08 095 1.11 018 0 86 1.20 004 14 12-03-02 1 02 1.03 004 095 1.11 0 08 0.95 1.11 018 0 86 1.20 004 15 12-03-02 1 03 1.03 004 0.95 1.11 008 095 1.11 0.18 0 86 1 20 0.04 16 12-04-02 1 02 1.03 004 0.96 1.10 0 08 095 1.11 0.18 0.86 1 20 004 17 12-06-02 1.03 1.03 004 096 1.10 0 08 095 1.11 018 0 86 1.20 003 18 12-11-02 1.04 1.03 003 096 1.10 0 08 0.95 1.11 0.18 0 86 1.20 0 03 19 12-18-02 1 04 1.03 0.03 0.96 1.10 0 08 095 1.11 0.18 0 86 1 20 0.03 20 01-07-03 096 1 03 0.04 0.95 1.10 008 095 1.11 0.17 0.86 1.20 0 04 Note: 7-d ICs 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT Central tendency (mean IC25)
S - Standard deviation of the IC2 values SA.0 = Standard deviation corresponding to the 10 percentile CV. SA.o = 0 08, as determined by USEPA for the method and endpoint.
S.25s = Standard deviation corresponding to the 25th percentile CV. SA25 - 0.17, as determined by the USEPA for the method and endpoint CV - Coefficient of variation of the IC25 values Control and 'aminmg limts were established using the standard deviation ofthe IC2 values corresponding to the 10* and 25SapercentiteCVs These ranges are more stringent than the control and warning lirrts reconaranded by USEPA for the test nethod and endpoint LSEPA Recommnended Control and Warning Linits.
Warning Limit = Standard deviation corresponding to the 75" percentile CV SAs = 0 45 ControlLinrit Standard deviation corresponding to the g 0 ' percentile CV S&.=062 USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elirranation Program EPA-933-R-00-003 US Environmental Protection Agency, Cincinnati. Ol. 01-07.03 xis
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.5 -7 1.4 Warning andControl Limits Set According to 10h'and 25'PercentileCVs.
1.3 Q
1.2 . ....... .. ....
S1 .1 . ,. . .,.... .. .
- 1.0 0.9 0.8 Test date
.--- 7-day IC2 5 " 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC 25)
-... Warning Limits (mean IC25 +/- S A.10' SA.1o = 0.08, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC 25 +/- S A25, SA 2S = 0.17, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.5 I II I I I I I I I I I I I I I 1 I I I 1.4 P Control Limits Set According to J= 2 StandardDeviations.
1.3 F Z 1.2 1.1
-' 1.0 ............. .............. ...-.*........ ..
0.9 F ,..........
0.8 F I I I I I f I I I f I I I I I I I I I I a" eO, 0" 00, e4 0, 0,~rV 0> a& S '- Aý A '? 0"
,~, 'C) N , "Sq "'CV.
Test date 7-day IC2 s = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC 25)
Control Limits (mean IC25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Test Control Control Mean Survival Reproduction Cv CT MSD PMSD CT number for Control
(%) (offspring/female) (%) Reproduction (%) for PMSD(%)
CV (%)
1 11-01-01 100 27.0 6.5 3.1 116 2 12-05-01 100 24 8 9.1 7.8 4.8 19.5 15.5 3 01-09-02 100 27.5 7.5 7.7 4.5 16.3 15.8 4 02-05-02 100 23 1 10.1 83 2.6 11.1 146 5 03-19-02 100 26 3 86 84 3.0 11.2 140 6 04-09-02 100 26 2 123 90 2.8 10.7 134 7 05-07-02 100 27.3 81 89 2.3 8.4 12.7 8 06-04-02 100 26 0 9.9 9.0 3.8 14.7 129 9 07-09-02 100 29 5 80 8.9 3.5 11.7 12.8 10 08-06-02 100 28 4 104 91 2.7 9.5 125 11 09-04-02 100 31 4 67 88 3.0 9.5 12.2 12 10-08-02 100 31.1 9.2 8.9 2.9 9.4 120 13 11-05-02 100 29.5 80 88 2.5 8.4 11.7 14 12-03-02 90 34.0 62 86 2.7 8.0 11.4 15 12-03-02 100 33.2 6.0 84 3.3 9.9 11.3 16 12-04-02 100 32.5 11.0 86 3.2 9.8 11.2 17 12-06-02 100 29 7 13.7 89 3.0 100 112 18 12-11-02 100 33 8 7.4 8.8 2.9 85 110 19 12-18-02 100 305 7.0 8.7 2.9 9.4 109 20 01-07-03 100 33 2 81 8.7 2.9 86 10.8 Note" Average control reproduction (offspnng per female) = 29.3 CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 8.7% in Environmental Testing Solutions, LLC Lower CV bound determined by US EPA ( 10 thpercentile) = 8 9%
Upper CV bound determined by USEPA ( 9 0 thpercentile) = 42%
MSD =Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 10.8% from the control.
Lower PMSD bound determined by USEPA (10 ' percentile) = 11%
Upper PMSD bound determined by USEPA (9 0 thpercentile) = 37%
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH O1-07-03.xls
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 I I I I I I i i I i I I I I I I I I I 45 40 35 30 25 20 15 10 5
0 I I II II I I I_____ I I I I I I I I I I I I I I I I
-09 -' c? ~ "-)g -,
Test date
-- PMSD = percent minimum significant difference PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
- _ Central Tendency (mean PMSD)
....... Lower and Upper PMSD Bounds Lower PMSD Bound (10th percentile) = 11%, Upper PMSD Bound (90th percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
I(,
Environmental Testiniz Solutions, LLC Dne*. I ark Environmental~~~P ouin.LC~ Aetn I nf*A Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)
Species: Ceriodaphniadubia Date Time Analyst CdNaC ICR Test Number: L, Teststartj pt'cl"-" I 12c'c_o I..o, Testend o0It- tq-05 I tVS toI.
Dilution preparationinformation: Comments:
NaCI CHM number:. CARt 060O Stock preparations 100 g NaCI/i (dissolve 50 g NaCI in 500 mld deionized water)
Dilution prep (mg/L) 600 800 1000 1200 Stock volume (mL) 9 12 1400 15 18 21 DTount volume (ML) 1491 1488 1485 1482 1479 Test organism information: Test information:
Organism age: . O%. tL,* Randomizing template: O3AKP-Date and times organisms O-0%1-.0 CtIu -m ioiq Incubator number:
were born between: z.
Organism source: i7-.-\-o Ism f6 YCT batch: l _2-.___
Transfer bowl information: pH= 8.D) Temperature (°C)= 2S.2 Selenastrum batch: A16S CONTROL Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced c I 0 0 0 Adult mortality L.-7 IL.-j 0
Adult mortality Young produced I L 1 I_ J" C ___ 0i 1) o 3 Young produced [ 0 1 )
Adult mortalty [_L, tI _IL 4
Adult mortality Young produced I I- I.
(
L-L47 1
\-1J =t__
1 i*iiz 1 C) -L-I
\- T i ML f t f'-[% f% +/- # +/-
I i I 6
___ ~Adult mortality Young produced I_ Li I Z I11 7 [_______ued Total young produced Final Adult M ortalit, J -1J 1R~ 1 j 1
L ...-
.3 it-3(I.~ :
1.1C 29 t
~
IjI IS 3at 3 5(~5 .{
0
-y . .-. _=. _ _ .
Xfor33 Broods > ><
Test Renewal, Feeding, and Randomization Record Label Mon Tue Wed Thu Fri Sat Sun Date ouL:AJ5 aftLe o oLoj 6j1. 01-lici~ cai-.
Time 12C 1700 i1i LX IZ03 5 k= l1M Shelf ~ ¶- ~ ~
I Location Control information: Acceptance criteria Summary of test endpoints (mg NaCIIL):
% of Mal- Adults: :520% 7-day LC50 ':'4cO0
% Adults having 3 rd Broods: c-'7. > 80% NOEC fcoo
% Mortality: 0- 5 20% LOEC )Coo Mean Offspring/Female: 35. - 15 0 offspring'female 2! ChV 't q. 1
%CV: &.1tO. < 40.0 % IC25 jz 2_. q4
Environmental Testing Solutions. LLC Pane 2 of 6 I
CdNaCICR Test Number:
600 mg NaCI/L Survival andReproductin'Data Replicate number Day 1 2 3 4 5 V -6 7 8 1 9 10 I Adult mortality [L - . .I.- L 1 Yog m produced Q Ir) c) I o .t 2
SAdult mortality Youngproduced n 1
r) 0
!'le C 0 g Ir° C) C 0 Adult mortality L. IKL- L<CIZL-j I 'I U "-
4 Young produced 1_qls_1.IsF6 V.I._V j Adl mortality Itl_+/- : 1- JL.L1 6 Young produced 12\-KoTo LktnhI'~ic Adult mortality IL1 [_UI I L-f I~L i~L II 7 Young produced 0 IV Total young produced 3q K a Z5 5q I I a ,311 35 Final Adult Mortality I - C..C- C7 -- = - c. L.- U Concentration:
% Mortality:
Mean Offspring/Female: 35.2 0 I
% Reduction from Control:
800 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 Young produced 1_1 2 3 4 5 6 7 1 8 9 10 C> 0) G* n C) 1 (0- (-) I d :)
_ Adult mortality L_4 L-- L.-I- I--
-- IL_ I__
2 I Young produced _ 1_ r 1 n__ I lo !.. Il 32 Young produced I I I I I.bl I 0 I0 I 0 IC*
4 A mortality Adult fYoung produced 74
__ I_-_I L 11JS K 1LJL4B1~ I tu1 _ 1 1 Adl otlt I tZ L'.
5 6IAd Youngroduced ult mortality 6 YonTrdcd 4
Lt 1L_-IL 2-
) IC 1-Ij~ -
12-*,13 1 1 I
o2.
I 1
14 L
1L C.AtAt.'
0.4e&z.
7] Young produced 1-3 1-1 fL IO ~ o l C Total young produced I -4 7 3,Z 37' 3s 32.o I 335 Final Adult Mortality I 111 -=. t_.. =.__
Concentration:
%Mortality: Cy.
Mean Offspring/Female: !.k
% Reduction from Control: L4. 2 ,D
Environmental Testing Solutions, LLC Page 3 of 6 CdNaCICR Test Number: jo 1000 mg NaCI/L Survival andReprodiictionData Replicate number Day 1 2 3 4 5 61 7 8 9 10
!I Young produced 0 0 C) (7- CN o Adult mortality 1J ... t_. _. C. t.
z 1 Young produced 1C01 0 Ic r aT!O 10 0II Adult mortality It.-j_' .- '--- I I_
3 Young produced Young produced LI s 6 Adult mortality Young produced [ t1 ic.I I T%- 0 1Ij 1. Qi ___ __
.2J Young produced 01 ILA 0 )
Total young produced . zi v'
'I ti 2 .,9 I l, 2. 2, . _
Final Adult Mortality I ... t_7 TC -:- * '- I Ct' .
Concentration:
% Mortality:
Mean Offspring/Female" 23.1
% Reduction from Control: Zq.,
1200 mg NaCI/L Survival andReproduction Data Replicate number Day _ 1 2 3 4 5 6 7 8 9 10 I Young produced C ) (C) 0 o)0 (3 0 Adult mortality 1I.-. ' _I_ ___- __ L.-. L. L.
2 Young produced Adult mortality p
-- I L ._I j j o IL -I I6 01*0 t_
u 1__
3 4
Young produced Adult mortality Young produced t-J.. u[ IL* c1 lkiC 1 L1 I7 C) 4 Adultmortality t - t1 i I_ _ -I I 1 Young produced [0 1 I A40 01 1 0 I
__Adult mortality [L.I- jL. . L- JL- L- _I L I 61Young produced r) ~r j~ Q.4 Adult mortality L-U .LJ- _** J I I I IJYoung produced 1ýi5 I0 01 0o Total young produced a
.* "i r. q __ 0 '01
- S Final Adult Mortalityj [... I--. L.. L . - t....- - .--
Concentration:
%Mortality: I0 I I Mean Offspring/Female. I 5.A I I% Reduction from Control- 62-27.
Environmental Testin2 Solutions. LLC Pa~e 4of 6 g, . .. -- , . o4 f. 6.
CdNaCICR Test Number: (,
1400 mg NaCI/L Survival and Reproduction Data Replicate number Day 1 2 3 1 4 5 6 1 7 1 8 9 1 I0 1 Young produced J Adult mortality _ _ __.. L j C L- .
L -
L ~ I SAdult mortality d Z - _. .. , .
3 Young produced [ C )I I )
Adult mortality 4 Young produced Adult mortality L.Q L_ -CII _ _ 101 I o"-- c~I ____
1c)L
___ Aon rdutoreld C).. 1 -1 (-- I_ E _ _
Adult mortality -_ -__ I I t" _ _- _" _ _
6 Young produced L l0 IC) OL IC L )jc ol Adult mortality \. LLU~(~] ['I'I-7 ] Young produced 2 C) C)
Total young produced t I o C) tI o o I (3 Final Adult Mortality I L ..- 1-12 - L- .-. , T(7- .- L.
Concentration:
% Mortality:
Mean OffspringfFemale
% Reduction from Control. 95 .2%
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #35 Test dates: January 7 - 14, 2003 Reveiwed by: '-i /
Concentration Replicate number Survival Average reproduction Coetlelent of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/femate) vriation (*) control (%A)
Control 31 34 36 30 29 36 35 31 36 34 100 33.2 81 Not applicable 600 34 34 33 34 35 32 30 34 31 35 100 33.2 51 00 800 31 32 32 32 32 31 31 32 35 30 100 31 8 4.1 42 1000 21 27 19 19 29 29 18 25 24 22 100 233 178 298 1200 5 3 7 12 9 5 0 3 10 5 100 59 616 82.2 1400 2 1 3 0 0 6 0 0 4 0 100 16 1324 952 Dunnett's MSD value: 2 853 MSD = Minimum Significant Difference PMSD: 86 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnla reproduction by 10 8% from the control Lower PMSD bound determined by USEPA ( 1 0 thpercentile) - 11%
Upper PMSD bound determined by USEPA (90th percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for CerlodaphInareproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Environmental Testing Solutions, LLC Statistical Analyses Cerlodaphola Survival and Reproductio Test-Reproductlio Start Date 01-07-03 Test ID CdNaCICR Sample ID REF-RefTox&cnt End Date- 01-14-03 Lab ID MS-Env Testing Solutions Sample Type* NACL-Sodmn .chionde Sample Date Protocol. EPAF 91-EPA Freshwater Test Species CDnodaphma dubut Comments Cooa.mgfL 1 2 3 4 5 6 7 9 10 D-Control 31000 34000 36000 30000 29000 36000 33000 31000 36000 34000 600 34000 34000 33000 34000 35000 32000 30000 34000 31000 35000 g00 31000 32000 32000 32000 32000 31.000 31000 32000 35000 30000 1000 21000 27000 19000 19000 29000 29000 18000 25000 24000 22000 1200 5000 3000 7000 12000 9000 5000 0000 3000 10000 5000 1400 2000 1000 3000 0000 0000 6000 0000 - 0000 4000 0000 Transform: Untransortned Rank ]-Tailed Isotonic Cone-mg/Ia Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 33200 10000 33200 29000 36000 &.132 10 33200 10000 600 33200 10000 33200 30000 35000 5080 10 10050 7500 33200 10000 800 31.800 09578 31.800 30000 35 000 4140 10 9200 7500 31800 09578
- 1000 23.300 07018 23300 18000 29000 17.759 10 5600 7500 23.300 07018 1200 5900 01777 5900 0000 12000 61605 10 5500 7500 5900 01777 "1400 1600 00482 1600 0000 6000 132419 10 5500 7500 1600 00482 Auxiliary Texts Statistic Critical Skew Kurt Kolsnogorov D Test indicates normal distnbution (p > 0 01) 0644731641 1035 0 179333511 -0088421936 Bartiers Test indicates unequal variances (p - 8 16E-03) 15 57795048 15 08631706 IHypothesis Test (i-talL 0 05) NOEC LOEC ChV TU Steers Many-One Rank Test 800 1000 894 427191 Linear Interpolation (80 Resamples)
Point mg/L SD 95% CL Skew IC05 806 1176471 6223646999 6214058824 8310523374 .21157 ICI0 8451764706 1610286245 8145108696 8708034163 02593 IC15 8842352941 179163057 8500187974 9188172779 06136 10 IC20 923 2941176 21 52170053 886 7947958 9677859037 06574 IC25 9623529412 235811894 921 1981225 1005440864 01587 09 1C40 1038850575 1170680582 1014698685 1060114799 -02641 08 IC50 1077011494 9250837919 1059673238 1093762391 01241 07 006 0.5 o04 03 02 01 00 0 500 1000 1500 Dose mg/L Dose-Response Plot 40 35 30
.2 25
,o20 515 10 0
U 6
01-07-03 xls
Environmental Testin2 Solutions, LLC Paee 5 of 6 SlutonsLLCPame EnvionmetalTestng 5 of 6 CdNaCICR Test Number: (o Daily Chemistry: HA-9 6A-c H : o I-o6-6 IT Dav Concentration Parameter 0I Dav1 0
vI 1 I 2 I pH (S.U.) -42.96 t..
DO m )
CONTROL Conductivity
(_.unhos/cm)
Z,W'2...
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Temperature (0c) zq< Vq. q.- 1
- z. I 4*,
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800 mg NaCl/L Conductivity 1.1i4 (jimhos/cm)
Temperature Zq-q " ,4.,. 1- 7-q . Zq.. .
(°T)
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1200 mg NaCI/L Conductivity Zq*
(gmhos/cm) ?:6 25 -4 Tem perature . 2 Iq (0c)
CC.)
PH_____ e.ZH z 8,1 qA.Iz.1 8-M-1 Z .
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Temperature ZY.q Iq -q- -LsR ZL.~ qq 2 ~ Zq.'7
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