Text

Revised Toxicity Biomonitoring Report for Sequoyah That Was Included in Discharge Monitoring Report for October 2002
	ML030410121
Person / Time
Site:	Sequoyah
Issue date:	01/17/2003
From:	Beavers M; Tennessee Valley Authority
To:	Wood R; Document Control Desk, Office of Nuclear Reactor Regulation
References
	Download: ML030410121 (79)
	v • d • e

Tennessee Valley Authonty, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 January 17, 2003 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Attention: Mr. Roland Wood TENNESSEE VALLEY AUTHORITY - REVISED TOXICITY BIOMONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Enclosed is the revised Toxicity Biomonitoring Report that was included in Discharge Monitoring Report (DMR) for October 2002 for Sequoyah Please contact me at (423) 843-6700 if you have any questions or comments.

Sincerely, Michael G. Beavers Acting Environmental Supervisor Signatory Authority for Richard T. Purcell Site Vice President Sequoyah Nuclear Plant Enclosure Prmnted om recycled Paper N

Sequoyah Nuclear Plant Biomonitoring October 08-15, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets N

1, Environmentil Ttmnu nhus;nn. TYr'

--Page ý1'I Sample Receipt Log Receivedi By Received From Temperature Project Number Sample Number Sample Name and Description

- I I

'~'

II p

0 AIfK&.

be

414:1 fl7IAilJ Al 02,1001. CZ~

OZ2(602.0 I bziooz.03 021062-0q' (OZIooq.o I o; 100b5, boi b;Lt6e.01 I

1

- 01

-re5ý-A-.

fuei1 01 T

I

__7I I. W~

I T

t

+/-____I 1-J I

I rebeX Febc t

I L).6 *C V~-*

A/74 q757 q-pL q8I

-~/w~ib.-rg-TAtHieL

jmrv4 FcLest-IdvL 1069 q~?0 18d-I.

.C-1 5 of otwt-,8bq Fed 011 kLJ-

ose.

L/63 o z.ic7.0Z.

+

State N~c.

NC.

6(c M

Jc-Comments

072, t-ol (0 Aft.

466 0 V00

/Ylaffaa.aLh Jý

'AI C.

0 z~yf. o3

-' ivIg4fYrP4o4.DJ..

a-r 4

4

ESZP

P.1t1J11 I

I I

I qtv

,..rr.

All' Fed q67 OA/001.0 I

Ivc-ateI Received rime Received 105-L O1.

or4

kEKeet~~~4imL Meet,4Ai KE Kc t

"1 'V

'ZOO 1 7-W

-314D I US i.

10-0,7.0-2.

10.02-02.

10-O2-07, A

-o~2 I0.oS.0

ko6.oz Io t5z

10. 802

/O.06.bZ 16 6 16.09.0Z.

IA A/

&U'Oql' 1

/0 tA_ 07

/t,4z K4 kaA-WCdJLI$

/6 '4 OVMbr4 111)/

0. 6 C boxe ffm.-ezq Corer.

Asaewue..

Ngj

AFa GeOE~T-OU~

4S-vM.,

k%%Pekdec 3

Sem S-r..Ghi fCS 9IAGSP-4 ATr

& A:FL-J0 L)~i tvludeco

£,

02"kae-LLrP I.q L CU&&o Fe d 0.6

?.q r C-

%M4nA_

eq NJ C.

N C.

aNS I

I T

ý

ý O.qc.

tIf C ftJC

Page 6Z4-Environmental Testing Solutions. LLC Sample Receipt Log Received Received Sample Project Sample By From

. re Number Number Sample Name and Description CQ iNWAI"ýT4.,

41-3.1-C 488 oziooq-L-6.

Iýed L 0 C.

'f(59 OzI009.0t.0 4,,j C). Orc, OZ1010.01 kr

ý&80eo KEG-eisA, Ge-iz:,ey, S3 02-1010-oz -Ivf.A-C)ar--f=OýU-101 KEKeeo,3A Te b 6,2 'C-t4,e S oz 10 1 o. o4s -rV A - I mT^I

e-6 go wy-Trp Aurtb vF-vp-e#j PQ E9T 2 C-499 IkAF kc-au KE4WAO C.>Cý,4 3-*3*C-4S4 0 ?-10 12. 05 KEVe-et4r-,Q C 14S-7 021012..0(0 'TVA,- SejsýL,.NcA A,ýk YLEV,-WAtý o-zlo 12 01

2. q -C-TVIA- 'SP-WX-Oý\\ P11OCk LA ýTr 10 1z C)8

]Nrm". 3 :;ýp OZ106.01 CU:Rý PAF-hA-A -rVA-PRIF-11,4%!, 49 1 Ovols.c)z

PA C-,D - A t), 0 edby

-C 1ý C)ZIN5.03 U-J-ti Onck S(K-ý d,4-Xd O-ZIC-ry COePJ24LJIOe% 02 -IW4 Cr IbI6.CI W L M 1-. -51 0:1 ýC pq 0ZLDI(O-Oz UIOM &I'"I cbapoacb.OV, Mt601& ()dOJAW-gfaýO,-, State Comments Mc ,5 C t4c-U-PýnAA04* TKI S C TI( Ký r-SCI Lrr-,( NX, NC NC. Nc N C FQ Kq Kq 11 C. MCI Date Time Received Receive IO.Oq4z 1 /0 1 6,,0'7-6Z-1161 10-10 1 C)SO 16

10 1 OSZ 10-10-6z los-z 10-10-()-Z.

IOS9 10-10 ltc)o to-11-0 IqqC) 10- 1-2 -0 2 16-12-07 lo-12 110LA 10-1Z a?- 110S 10-IZ 1105 __nC 6ýE I 1040-Oz (A IIOMONITORING CHAIN OF CUSTODY RECORD Page I. -of I Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One): Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify): __ Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments: NPDES Number: 0026450 Fax: 828-862-8195 Collected By: RONALD LOWERY Container l Field Identification / Grab/ Ship. ColcinDtlie Number & Flow Rain Event? Sample Description comp. Temp. (Volumre (MGD) (Mark as Appropriate) 1' .. 9Collected -CA%2 ~ ~ i5

~~Stant If Yes, No Trace B~ST LOS A1*!**T( fly -

'hw p' 02 Z.. '-k.,..;,4 SQN-101-TOX conip 4 10/602 1//2 2(2.5g) 4 SQN-INT-TOX comp 4 10/6/02 1070 l(2.5g)~ C'N V. I ~ Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): DatciTime Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomionitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time flame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. BIOMONITORING CHAIN OF CUSTODY RECORD Page I of r1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One): Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_ Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments: NPDES Number: 0026450 Fax: 828-862-8195 Collected By: RONALD LOWERY Container Field Identification / Grab/ Ship. Collection Date/me Number & Flow Rain Event? Sample Description Comp. Temp( Volume (MGD) (Mark as Appropriate) S0C) Collected .star End Y. If Yes. No Trace-*..V.l.. EST Inches SQN-IO0I-TOX comp 4 10/8/02 07*' 2(2.5g) 02 R:: 10/9/02 SQN-INT-TOX comp 4 10/8/2 1oo 9 1(2 5g) / C..

S

-"°::::. :"" CC. f:. ""*" ",*. Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time __e__________I__ o-,O'" l6$Z- /QoS2_ /.* /'51 - Vt I, I hi tructuons: Clients snoula ill in all areas except those in the "Laboratory Use" block Biomonitoring samples are preserved by storing them at 4C and shippfi'g them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to'initiate testing ivithin that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. 1 I BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of 1I Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One): Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_ Facility Sampled-SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments: NPDES Number. 0026450 Fax: 828-862-8195 Collected By: RONALD LOWERY Container M Field Identification / Grab/ Ship Collection Date/Time Number & Flow Rain Event? Sample Description Comp Temp ( Volume (MGD) (Mark as Appropriate) .A` C)______ Collected M.. X Start End Y-I e, N Inches T e .......x x f1291 10/11/02 M. 1 .cs 10/10/02 ,Z.-7*'7 2(2.5g) ...*S ::: : A::***: i:*::,:.*.*:* SQN-101-TOX comp 4 10/10/02 /.7_ 2(5*), 1~2 /--2-1 C>__ SQN-INT-TOX comp 4 10/10/02 10/11/02 .(2.5g) i i.. 13112-Sample Custody - Fill In From Top Down Relinquished By (Signature): DateTrime Received By (Signature): Date/Time 'I // / 7 1-.4- -I r i itMt .II UISMruCions: .inents snould fil in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4*C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday. Environmental Testing Solutions, LLC Page I ot 8 Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0) Species: Pimephalespromelas Client: TVA Facility: S<POSAI-IMcr.igA OPA.JT" NPDESN#: Tt -,,,.L Test organism information: -.-- 277. S Organism age: 4t'81V1 4 id Date and times organisms 10-01-02 10B -ý r3ao )Al were born between: S $30 DT Organism source: A A l0-"-.. Transfer bowl information: pH = Temperature = __z'.I Comments: Test information: Randomizing tern late: Incubator number: Artemia lot number: Oven temperature: Drnetie Diving time: Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 to-o8*.oz Io(n i~ss9 A( 1 Jo-C+07-. 1021 1(05o 1S 57 2 l O-Z-U Oi1S 3 160 0 115,5 ZI 6 1(0-1.! z 7 1 q jq. t/A Control information: Aeptce cntera % Mortality: 2 S 520% Average weight per larvae:

0. q'/03

> "25 m2Jlarvae Summay of test endpoints: 7-day LC50 O NOEC 1/of. U I LOEC ChV IC25 1C25 Dilution 'raration in ormaton: MHS batch: /0.04 Dui* n prep0(%) 2o.q 2z l q "z 00 Effluentvolume (mL) J~'.7 330 Io5.& W030 i 5c O Iluert volume (mL) /3531 1 C I br IC -Ic Z 3 61 02-"C-, >/1oo 17 W0072% bD Coi*nents: I 9 I Species: Pimephales promelas Client: "vlA-

-5o

Iow_,

H No ,Cede Date: /1.-6662. I 1 'ý Survival and Growth Data _ Day CONTROL -6 .& 7o A2% A B C D E F G H I J K L 0 10 /0 10 /0 1 10 b0 /0 10 I /0 1 IDo 10 10 o(o I o 1/( 0 /0 /0 2 Jo /! /0 /0 Io 10 /0 10 10 1c1 10 I0 3 /0 /0 /0 10/I0 /0/ 0 /0 /6 10 /0 /0 4 /0 /0 to q14 0 t0o /o to /0 /0 /0 6 1o 10 /0 /0 1 /0 /o 10 /0 /D /0 /0 /0 6 _o__0 /0 61 0 10 1 A /0 16 lo /0 /0 7 l o j X) 9 A) to0 lo R) t01o 10 P A- -Pa -w-igh-t BPan+Larvaeweight(mg- ) All____ 16,0 a-32-. 11 q.i 2 16.4 2-o24a'0nN 74 7'3.o i5,45 Lmveweight (mg) - A -. B Ct( cj I :l~~I2 Ij~ o s 45{4 I q.*v 5..oqo iq 4 0 ~ 1 -1 Calculations and data reviewed V I' 'C Comments: v...nmental Testing Solutions" LLC Xi Z 01 0 " I niironmenrai I esung oIunons, LLL Page 3 Ct S t-. Species: Pimep hales promelas Client: :ý70- - '*q, &4 Ij A)oN-r~e.ArPb Date: 16.06.0Z '.5', '1 -,, t ~~Survival and Growth Data _ Day 3

10f9,

?--u)bg _M N 0 P Q R S T U V W X 0 /0 ICo 10 /0) /() /0C) It) i I /0 R-) /0 1 /C) 10 1 C 10 10 10 10 £0 10 IC) /V /6 3 Io t10 /o0' /0 /(0 /(0 /0 /0 /0~ /0 /0j 4 /0 /0 /0/0 lo / /0 1vl /0 /0 /0lo/0 5/0 I)D /0 /0 IL) IL) /0 /D /0 /0 /0 l/0 6 /O /0/0/0 /0 / /0 /0 /0,01 0 /0 7I0/ L) /0/ to) /C) /0(I0 A - Pan weight (mg)- -S B -Pan +Lamve weight (mg) z ~ez. ~'72o z.3?3 I st ~ ILarvae weighit (mg) -A-B O Ir L 7 qO'2i 014, 2 S 20~' 0.73 S Illq ic0.05 1 I.4' Calculations and data reviewed. Comments: L imninenvu I esting bolunonsLALU Page:$ ot 8 I Page 4 Ot 8 Species: Pimephalespromelas Client: 7~A-ae&~y MmrgzTeb Date: /1.08.62 Day U 1 2 3 4 5 6 7 A-Pan weight (mg) B - Pan + Larvae weight (rug) Larvae weight (rmg) - A - B Survival and Growth Data L k/OO2I Y I AA -

IR i0

/0 /0 1 /V~ /(0 Jo I0 /o 15Ai f' 10*

10. 'Io '

q7d'I I j - 10 to /0 /0 /0 IC) I0 to /0 /0 /()j zs. 12 It) I0 ID 1o /0 /0 ,c) 24.31 Calculations and data reviewed: Comments: br 10 L)C IC /C (0 Y , OP , 6\\* r

o

Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0) Species: Pinephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated October 8-15, 2002 Project number: 433 Dunnett's MSD value: PMSD: Dunnett's MSD value: PMSD: MSD PMSD= 00936 100 00882 9.4 Reveiwed bi ~- Mmunum Significant Difference Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent differete between thee oinlr and Umr did can be declared statistically significant in a whole effluent toxicity test. On svensge, a significant differeice occus for Enviromnmetal Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19 6% from the control (determined through refertnce toxicant testing) Lower PMSD bound determined by USEPA (10th percentile) - 9 41/. Upper PMSD bound determined by USEPA (90th percenttle) - 351/c The lower and upper bounds were calculated by the USEPA using 205 teats conducted from 19 labostakirs fdr Pimnehalas growth in chronic mrerence toxicant tests I I USEPA. 2000. Understanding and Accounting for Method Vanability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPAJ33-R-00-003. US Ean'vlmmntal Protection Agency, Cincinnati, 0il Client: Test dates: Concenralion (%) Replicate Initial numnber of kinal number of A - Pan weight 8 - Pan + LarosLaa ti(mg) Weight I inlaial aumbar Mes survial Mess weight (0a 'umT a I larvae larvate tn) ewight (trg) - A - oft larvae t(mg%)CA ) () a (%) A 10 10 14973 24.790 9817 09817 Control B 10 10 15 143 25020 9877 09877 97.5 0.9403 6,2 Not appable C 10 10 15056 23680 8624 08624 D 10 9 14 817 24.110 9293 09293 E 20 10 14940 24210 9270 09270 1098% F 10 10 15000 25 190 10190 10190 1000 09400 7.8 0.0 G 10 10 15 170 23620 8450 08450 If 10 10 14741 24430 9689 09689 2 10 10 15 177 24760 9583 09583 22% J 10 10 14710 23_750 9040 09040 1100.0 0.9204 23

2.

K 20 10 15054 24050 8996 08996 L 10 10 14732 23930 9.198 09198 M 10 10 14908 24990 10082 10082 439% N 10 20 14866 24870 10004 10004 100.0 0.9953 4.3 .- 3 0 10 10 14648 25020 10372 10372 P 10 10 14855 24210 9355 09355 Q 10 10 14942 23970 9028 09028 72% R 20 10 14886 25060 10174 10174 1000 0.9192 6 2.2 S 10 10 14665 22930 8265 08265 T 10 10 15071 24370 9299 09299 1 U 10 10 14925 25 160 10235 1 0235 100% V 10 10 14819 24610 9791 09791 1000 0.9952 2.3 -.Si W 10 10 15 193 25230 10037 10037 X 10 10 14665 24410 9745 09745 Y 20 10 14950 25480 10530 20530 100% Intake Z 10 10 14663 25560 10897 20897 100.0 1.0279 6.3 -9.3 AA 10 10 14714 25 120 10406 20406 BB 1 10 10 15089 24370 9281 09281 ,1 Environmental Testing Solutions, LLC Statistical Analyses LamE RA Grow& sad Sw~arta Tua.7 Da Growth Smns Dam 10=. Tom ID' PPM=C Sas&WOOD). TVA.SequsyahsNuIwlnPlum End Down 10/15M0 Lab a) 23EM&V. Taftn SoleAm SanghTYPS MM~43IU tp M-ftwift Rap-t SRvkDin ?MsOwI EPAP 9147A Fseemawte 7Te Spede. P?-plsnqpua pemskdu Cmc-% 1 2 3 4 D~cmaw 0.9817 0.3M7 0.2624 0923M 101.98 0.927 20190 Om"5 0.9689 22 Um88 0-4040 0.299 0.9198 43.9 1.001 I.m 1.072 0.955 72 0).902 L.0174 0.226 0.99 too 10235 0"971 1.07 0.945 Traaeferin. Ue~mfemad -TaildIaal C40e.% Mess N-Mes Mes MWa M=s Cv% M 6-swa Cuidmi MSD Mm N-Mes D-Cors 0.9403 1 ID 0.9403 0.962A 0.9m7 6.186 4 09517 1.m 10.98 0.94M0 0.9m0 &.9400 0.8450 10190 7.835 4 0.008 21410 0.0936 0.9517 lim 22 0.9204 0.9789 0920 0"%99 0.9583 2.901 4 0.511 2.410 0.0936 0.9517 Lm 439 0.9953 10513 09953 09355 10372 4.311 4 -1.418 2.410 00936 09517 1000 72 0.9192 0.9775 0.9192 03265 10174 1m7 4 0.54 2.410 00936 09517 1000 t00 0.9952 105S4 0.9952 09745 10235 2M29 4 .I1.45 2.410 0.0936 09517 t DO Aaxhllry Taft Sftaulslc crkesial Skew Kans Shosro-Wsiks Teat auxbceam sanal dsmbon (p > 001) 0.959660053 0014 -0.237724829 0.135401943 Hxdetes Test nssiesze equal vu=a (p =0.303 6.020889282 1508631706 Hypetels Teat(1-eshL 0-MS) -NOEC LOEC CkY TU MSDu MSI~p MSB ME50 F-Prob dt Dimnness Test 100 >100 1 0.093566423 0 099509636 000D4880827 0 003014643 020562432 5.18 Usea Italapel~sil (90 Resanpkie) POWm SD 9"% CL4Ezp) Skew IC05 >100 [dlO >100 ICIS >100

10.

IC20 >100

09.

1C25 >200 IC40 >100 08.0 ICSO >100 0.7'.

06.

a0.5. &04

03 Ix0.2 01 006

p

-01 -0.2 0 20 40 60 s0 100 120 Dose % DoweRespmo~s Plot 1.2 1 -tag,. 0 05 level 08of sigrnficance 0.2 sqqjO.-082data~xls Environmental Testing Solutions, LLC Statistical Analyses LjrvaJ FiM Growth and Sarvtval Tesm7 Day Growth Start Date-10/&M2 Test ID PpFRCR Sample D" TVA. Sequoyah Nuclear Plmt - Intake End DaIe-10/15102 Lab I) ETS-Env Testing Solutons Sample Type DMR-Dudhnge Momtong Repor Sample Duze Protocol EPAF 9I-EPA Freshwater Test Species-PP-Ptnephales promel. Comments Comac-% I 2 3 4 D-Control 0 9817 09877 0 8624 0.9293 100 10530 10897 1.0406 0 9281 Transform: Untransformed 1-Taded Conc-% Mean N-Mean Mean Mm Max CV% N t-Stat Critical MSD D-Control 09403 10000 09403 08624 09877 6 186 4 100 10279 10931 1.0279 09281 10897 6780 4 -1929 1943 00882 Auxiliar Tests Statusc Critical Skew Kulr Shaptxz-Wtik's Test indicates normal distibuton (p > 0 01) 0877389908 0749 -0938263685 -0.532798057 F-Test indicates equal variances ip -0 77) 1435506105 4746834564 Hypothesis Test (1-tail. 0 05) Homo*c*datic t Test i-dicates no significant differences sqnl1-0O-O2dataxfr niTesg Solutions, LLC Page I at 8 Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1002.0) Species: Ceriodaphnia dubia Date Time Analyst 10.Lw0Z2. 1 lizl I -l I i16 -z, 1 1sls "It I Client:-D w - OOZLO'a4a a 5.-0 Facility: %J I NPDES #: "T'N - QQZtP4S0 Dihution pparatio n ormatlon: Comment: DMpM(%) 10O8 Izz ,41.0 qz t0o Ei vo (ML) ileq.-4 - W.5 I0go Dihm'vOh=(=) 1*{) 140 &41.6 84Zo 0 Test organism information: Test information: Organism age: /21 ha bC6OIC Randomizing template: L.q0 Date and times organisms jC-Cro-O 2. C4ISMo -r IO'i Incubator number-. were born between: __2 Organism source: }lo -oo2 Av4.6 YCT batch: I8t) O.0tZ Transfer bowl information: pH= e.dq Temperature

t.

Selenastrum batch: A651 ,0.o' ioz CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced O O O O O O Adult mortality L L L LI-2 Young produced C) 0 0~~j IQ Adultrmortallty t

6.

3 Young produced [6 (41 L t Adult mortality L...-J, = Z-,pd, = I- - I I - = { / l

It 4

Young produced

17510

.r7I GŽ Adult mortaltL 1 I = I t= E- = Young produced cc) 1 =- i Adult mortalit %I-1i 6 Young produced 1-J 1 I t 1314 Adult mortality U L.-__ 7 Young produced Total young produced 1,-5 50 1 30 2 30 2-1i

2 9 Final Adult Mortality L.-_

X for3' Broods Calculations and data reviewed. Test Renewal, Feeding, and Incubator Location Informaton Mon Tue Wed Thu Fri Sat Sun Date /O*. g0.A 10-10 /1A L I -La IQ-i-i Thie I 9 6 Lw lQ 13-f_(.L 12-= Shelf £4 C CL

t.

LL. L Location Control information: Acceptance cteria Summary of test endpoints: % of Male Adults: 2o 7-day LC50 >Ion7, % Adults having 3rd Broods: q1 ? 4/,, 80%/. NOEC

0.

% Mortality: 60<0/!% LOEC 0 Mean Offspring/Female: "2 q I. 15.0 offipmg/female ChV % CV: I__ < 40.0 % IC25 >100 Test start [ Test end I - -1,., -2. ý-_. --1 " ý,

c

.a,4aa L IAAI hUhULIUM. JJLA. I'flO 7 nt)( Species: Ceriodaphnia dubia Client: "V'". 5eaw*gh Date: lb.oa,& CONCFEfMTION: Survival andReproducton Data IDy 1 1.2 3 4 5 6 17 9 10

.*r-u t_

(2--.Q_ Q. L_, Q_ t._ 0 I Young prdue 0 I ) fil

1 I

O I

01.

Adult ortalty L. L L, L t L 2 Young produced 0 0 0 Adult morta ylty 3 Young produced a oA .5r Mdumortzl:ty L %Young produced from Control: c Adult mortality C r e 5 Young produced J12- / '9 10 1 0 0 i Q /0 IAdultmortalit L-L-k- = 6 Young produced 'I -~d L : 7 Young produced Total youngproduced 3 z 2S

301, zi 30 31 Final Adult Mortality 7

?

L i I= UC I 7 Concentration: I% Mortality: I'. Mean Of"-pring/Fniae: 7 % Reduction from Control: - q 4. Calculations and data reviewed:4L CONCENTRATION: ZZ 1-Survival andReproduction Data 1 ~Replicate number D a dul mortality4 1 5 6 7 8 1 9 10 1 Young produced 0

0.

0 0 0 0 C) 1 Adult mortality L L L 2 Young produced 0 0C Adult mortality I.-~ 3 Young produced 41 1 41 C) q F s1 5 ý Adult mortality_ 4 Young produced C) jG IL j0( J)Iz Adult mortality j

2. -k..+/-

6 Young produced 19 1~ 1-.. 17a£LIIR I Adult mortalityL 7 Young produced Total young produced S3c& 1 , 0 3 241 21 37 3,3 33 ZI Final Adult Mortality

C I

E T7 Concentration: % Mortality: &Z. Mean Offspring/Female:

31. 6

%Reduction from Control: I"1.37. Calculations and data reviewed: 4.- .tu I 11111,I,111JI l;llill*O~ilLl&J J1U,. 1)o ft V 4 C" r men Cs ng outions, LLC Page3olli Species: Ceriodaphnia dubia Client: -T7Y A-w.qt~ Date: /10-06.02 CONCZNTRATION: '4... Survival andReproduction Data Day_ 2 3 4 5 6 7 S 9 10 1 youg produced 77 Q rQ 0 0Q 00 Adult mazta-t L L. L-L- L-C, (I~ U T 2 Young produced p p p 0 0 0 3 Young produced 44 q Z Atult mortalty IL .... I L - L..-4. L. L-5 youn .produced... .C*. 1... iz.

1.

0 Adult mortality L .. o L. I-2

k.

I Y,ongprod _ aed. I t,, f Cl Iq eL4 +/-l,. L* IL, Autmortalt L.. t.- L._ U 7 1 L 6 Young produced Ii I Aduitmortality U L. 7 Young produced Total young produced 3~Z4 30- 341~ 3 0 J q 3 t S 30 15 Final Adult Mortalty 7 -_= F I L. L I L. I Concentration: % Mortality:

07.

Mean Offspring/Female:

32. 21

% Reduction from Control:.9 Calculations and data reviewed: CONCEZNRATION: Survival andReproduction Data Replicate number Day 1 21 3 4 5 6 7 8 9 10 I' Young produced C) c) 0 C) L* L ,) 3 ) Adul mo°rta"i"1ty Iil C-L- L::L--

1-L<",

2j Young produced 0fC) nn Adult mortality L . L.. 2 Young produced ) 4 14 Adult mortalityH Adultmortal*ty L 3 Young produced 16 13 17-1 11, 2 6 AduMorta t L L.- L L 6 Young produced 7-0 e ~ 2.4)__ Concentration: J Auitoraily% Moralty Mean Offsring/Female: ,q.f. %CReduction from Control: Calculations and data reviewed: 4. Lnvlronmental Testinl Solutions. LLC Species: Ceriodaphnia dubia Client: 7?i- *lea* Date: __.,O_._ CONCENTRATION: /04 Survival and Reprodudion Data Day 1 2 3 4 5

6.

7 3 9 10 1 Young produced 0 Q Q 0

7)

Q 1, 0 Adult,,ortal L-L.. L_ L- - I L_. L Adult mortality I-' 3,Yomug produced L. I d5 4 Young, prdue C) 00 0 (0 101 5 Adult mortality t ~ 3 Young produced 17.I - d u. L-l- LL C 7 Y,*,i p~l-* I 6 Young produced 1 1 6 1 zi 21 7 0 1 -0 Adult mortality 7 Young prOduced Total young produced 25 a 20 Z31 751 41 Final Adult Mortality C E -Concentration: I% Mortality: Mean Offspring/Female: 34.8 % Reduction from Control: -21.0 Calculations and data reviewed: _. CONCENTRATION: J rj-# / /

b.

Survival and Reproducion Data Replicate number Day 1_1 2 3 4 7 6 8 0 9 10 1 Young produced D 0 0 0 0 o -o 0jO 1 = Adultmortality L,. (_ I L-U I U 2 Youngmproduced 0 0 ) 0 L C_ _ Adult mortality UL L1 I t L_ L_ 3 1 Young produced 1-.. 4 T Adult mortality L L 7 Young produced TonYoung produced )I 17 &)3 ~. 2-- 2S /0I~'z' Final Adult Mortality tL t L L Concentration: 1 % Mortality: Mean Offspring/Female: Z7. I/ % Reduction from Control: Calculations and data reviewed: dl Client: Test dates: Project number: Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated October 8-14, 2002 483 Revelwed by:__________ Concentration Replicate number Survival Average reproduction C -e o f ft i* o ridudm f*-o (%) 1 2 3 4 5 6 7 8 9 10 (%) (offspringlfemale) wd () eni* (*A) Control 32 25 30 13 30 29 30 29 28 28 100 27.4 19.6 Not applkable 10.98% 31 32 31 25 30 15 29 30 31 32 100 28.6 1&1 -4.4 22% 36 31 30 29 27 37 33 33 29 31 100 31.6 10.0 -15.3 43.9% 32 29 30 34 30 39 34 35 30 30 100 32.3 9.8 -17.9 72% 33 32 36 38 31 37 34 33 30 37 100 34.1 8.1 -24.5 100% 34 36 36 33 39 35 20 37 37 41 100 34.8 16.4 -27.0 100% Intake 32 30 30 32 27 20 33 14 28 28 100 27.4 21.8 0.0 i 3o T II I 1 2 Outfall 101: Dunnett's MSD value: PMSD: Dunnett's MSD value: PMSD: 4.496 16.4 4.413 16.1 MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 11.8% from the control. Lower PMSD bound determined by USEPA (lOlt percentile) = 11%. Upper PMSD bound determined by USEPA (9 0 th percentile) = 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceod'phnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-03. US Environmental Protection Agency, Cincinnati, Off. Environmental Testing Solutions, LLC Statistical Analyses copais Saryn sad R-pro wedud Tmest-.Rajlactl Sta nDa ioe3o2 Tint 1D C6M Samoa D" TVA. Sezaoya Nuc lnt End Dom: 1011412 LabD-IE'S.Ewv. To*i SoWu SamoapTY D&M-Dichmp Mostoing Rep-t Stape Dae PMWsoeL EAP 914EPA FPriunur Tot Speces: CD.Cioclsomta dlsa Came.% 1 1 3 4 S 6 7 S 9 10 D-Coomi 32.000 253000 30000 13.000 30000 29.000 30.000 29.000 28.000 2.000 10A91 3L.000 32.000 31M00 25.000 30.A0 - 35.000 29.000 30.000 31.000 32.000 22 36 000 31.000 30000 29.000 27.000 37.000 33.000 33.000 29000 31.000 43.9 32.000 29.000 30000 34000 30.000 39.000 34.000 35.000 30.000 30.000 72 33.000 32.000 36000 31 000 33.000 37.000 34000 33.000 30000 37.000 100 34000 36.000 36.000 33.000 39.000 35.000 20.000 37.000 37000 41.000 Tramsform: UUcansformed Rank I-Tailed litoe COeC-% Mean N-Mean MISS min Mal CV% N Sm Crituil Mmt N-Mea D-Coogtol 27.400 10000 27400 13000 32.000 19.631 10 31.467 3.0000 1098 28.600 10431 21600 15000 32.000 31346 t0 123.50 7500 31467 L.0000 22 31.600 1.1533 31.600 27000 37.000 10029 10 132.50 7500 31467 10000 439 32.300 1 1718 32300 29000 39000 9796 10 139.50 7500 31467 30000 72 34100 1.2445 34100 30000 38.000 8 114 30 351.00 7500 31467 10000 1300 34 800 1.2701 34800 20000 41.000 16.357 30 14600 7300 31467 10000 Audliary Test Statk Crticl Skew Kart Kslmogorov D Test tadcaes non.-norml dutmbuton (p <- 0 031) 1.058434725 1035 -3.14435828 4 819992863 Basdte's Test indicaues equal va-ces (p -0 13) 8.516535759 1508631706 Hypothesis Test (I-tod. 0 05) NOEC LOEC ChsV TU Steers Many..Oe Rank Test 100 >100 1 lInear Intirpohlon (10 Reanples) Point SD 95% CL Skew IC05 >100 ICIO >100 ICIs >100 10 1C20 >100 09 1C25 >100 08 IC40 >100 0 7 ICs0 >100 0 6 0.5 0, 0.4 0.3 02 00..e S -01 -02 -0.3 -0 4 0 20 40 60 80 100 120 Dose % Dowte-esponse Plot 3 0 .2 u25 20

o.

15 10 5 N 0 8 1, sqnI_1O-O8-O2data.rLs Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD cakcutdon only Cerlodaphsah Surhvial and Reprodution Test-Reproduction Start Daze: 10IM82 Test ED CdFRCR Sample ED IVA. Sequoyah Nuclear PM=t End Daze 10/14102 Lab ID ETS-Env Testng Solhtons Sample Type DMR-D-chamre Moniortag Report Sample Date. Protocol EPAF 91-EPA Freshwss" Test Species CD-Ceý dubLa Comment Coam-% 1 2 3 4 S 6 7 9 10 D-ConLro! 32000 25000 30000 13000 30000 29000 30000 29000 28U000 28000 1098 31000 32000 31000 25000 30000 15000 29000 30000 31000 32.000 22 36000 31000 30000 29000 27000 37000 33000 33000 29000 31.000 439 32.000 29000 30000 34000 30000 39000 34000 35 000 30000 30000 72 33000 32000 36000 38000 31000" 37000 34000 33 000 30.000 37.000 100 34000 36000 36000 33000 39000 35000 20000 37000 37.000 41.000 Transform: Untrnsformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Conrol 27400 10000 27400 13000 32000 19631 10 1098 28600 1.0438 28600 15 00 32-000 18t46 10 -0 610 2.287 4496 22 31600 1.1533 31600 27 000 37000 10.029 10 -2136 2287 4496 439 32.300 1 1788 32.300 29000 39000 9796 10 -2492 2.287 4496 72 34100 12445 34100 30000 38000 8114 10 .3408 2.287 4496 100 34800 1.2701 34800 20000 41 000 16.357 10 .3764 2.287 4496 Auxdiary Tests Stamtstic Crical Skew Kart Kolmogorov D Test indicates non-normal dismbunon (p <- 0 01) 1058434725 1.035 -1844358283 4819992863 Bartiz's Test ndicates equal variances (p - 0 13) 8516535759 15 08631706 Hypotiess Test (1-ail 0 05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dutnet's Test 100 >100 1 4496034328 0164088844 8702666667 19.32962963 0001676433 5.54 sqnlO-8-O2daIa.xs Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculata* only. Cerlodaphual Survival and Reproduction Test-Reproduction Stan Date 10M02 Tet ID" CdFRCR Sample D" IVA. Sequoyah Nuclear Plant. intake End Dze 10/14102 LAb ED. ETS-Env Testng Sohmlos Sample Type DMR-Dutchnre Momtormg Report Sample Date-Protoc*L EPAF 9 I-EPA Freshwater Teat Speces CD-Certodnphina dubui Comments Cow-*/. 1 2 3 4 5 6 7 9 10 D-Contml 32000 25 000 30000 13000 30000 29000 30 000 29000 28000 28000 100 32000 30000 30000 32.000 27000 20000 33 00 14000 28000 28000 Transform. Untransformed I-Tailed Cone-% Mean N-Mean Mean Mm Max CV% N t-Stat Critical MSD D-Contol 27400 10000 27400 13000 32000 19 63 1 10 100 27400 10000 27400 14000 33000 21844 10 0000 1734 4413 Auxilary Tests Statistic Critical Skew Kurt Shptro-Wilk's Test indicates non-normal dutsibunon (p <- 0 0 1) 0 755762458 0868 -1 806076918 2-667645544 F-Test indicates equal vanmrices (p - 0 76) 1.238095284 6-54108572 Hypothesis Test (0-tad. 005) Homoscedas.c t Test indicates no significant differences sqnlO-08-O2data.xls Environmental Testing Solutions, LLC iage1ot Chronic Whole Effluent Toxicity Test (EPAI600/4-91/002 Method 1000.0) Species: Pimephales promelas Client: 7"/VA Facility: ,r jw b -0V17"(./ NPDES #: -r-. *. 30264 Dilution preparafton Information: Comments: MHS batch: 2_.O ____z Duhmopp(%) /o.g9 8 I'/Al I' 1z /0O Efflued voh=m (nil.) kt~rlb I'ii' aZ 43 1000 Dilued voume (mL) 04eaL186 ý0 r '26.Z0 0 Test organism information: 2.S h

Test information:

Organism age: Mhook' " a'O Randomizing template: XED Date and times organisms 10_0-0 T &350 3 rl Incubator numben were born between: 1 2-30 O 156o 30 C-nc Organism source: AS 8ATcA t-ol o-Z-Artemia lot number: 6 G Transfer bowl information: pH = Temperature = Oven temperature: I OZ C ""__7.q_ Sq. It"C Drying time: 11-40LW614 Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test Initiation, renewal, Analyst time time or termination time o0 oz -0? WC) 3 to-I0- 0"-- Oe*q 150-1IS* 4 I0Z-o'-O 1100 -L10() 1I1 I 5 Jo-13-oz 00-1 lýOo 15O 6 / 0.-*,- U A005." M CI I Control information: Aeceptl Ctea Summary of test endpoints: % Mortality: 20% 2o, 7-day LC5O > /00o j Average weight per larvae: 1 > 025, ae NOEC 1 _/00_7. ChV )/L)0r IC25 LOEC Environmental Testing Solutions, LLC nage lo*t 8 7!UViNnmentaI jesting Solotiont IT C n.ug zL a Species: Pimephales promelas Client: _-? Tv'j -,Sp iojA V -j Date: /O. O8.iz Survival and Growth Data Day CONTROL /6. q& 7. 2z'1, A. B C D E F G H I J K L 0 lb IO. 1/0 /0 I /0 zo /0 /6 16 /0 /0 1oI0o o /0 10 /0 /() /0 i /0 2 /0/ o /0 /0 A /0 // 16 / /0/0 3 /o /0 -( /0 /0 1 /D /0 11 /0 /__0 /D / 160/ /) /0 /D/ /0 b 1/0/0

5

/0 /0/D/0 /0 q 10 /0 /0 /0 1/0 6 J0 /, !1 lo /0 /0 /0 /0 /0 /0 7_ 10 /C) 00 9 0/ / / / 7___ I0o iU jo I0 / /0I /o0/0 IQ 1 9 A =Pan weight (mg) Inc t_ B=Pan+Larvaeweight(mg) 2*.,I .q* zq.7 iq.(, z.ll tq47 24 ( LarvaeCweight(mg)-Ai-oB 25d dta &i 11___05__________ it. 1 0 '.odoi 4qqp( 1,46 1 11.01 o50 gA.ZS Calculations and data reviewed: Comments: Environmental Testing SolutionnL fTTC Enviromenta,n Solutis -- Page 3 ot s Species: Pimephalespromelas Client: T'fl Lw -7eol Date: iO.od-,oZ Survival and Growth Data Day A3,/.q 'A" 0M N 0 PR S T U V W X

0) Ic) 1 0

c1) /0 /0 10 /0 /0 /c) /0 /D /0 10 /o /l /0 io/ I) /0 I)o 4/0 /0 /0 /0 /0 10 ,0 I0 /0 /0 /0 l0 /0 /I '0 I /0 /0 /0 0 /0 /0 /0 5 /0 /0 /0 /0/ /0 /0 /0 /o /0 // /o0 / 0 /0 /0 // /0 /0 /0 t0 60 loOI, /0 /1/0,0/0 lb 1/0 /0 /0 Me Id /0 16 IC0 10 10 10A)IL /0 /0 A~ ~ -a egt( g 61 B- ,Pan + -Larvae we, *gt(mg) ZO,.,' 25.V z'1.q( Z,.iO ",,.' ts z -.3 z 2017 2.S( *Z, i z.' L ight(mg)-A-B Calculations and data reviewed. Comments: I-I Environmental Testing Solutions, LLC P'age 4 ot s Species: Pimephalespromelas Client: 60*60-142b U V-77qe4kzd Date: /,04d56Z Survival and Growth Data Day 6 Y Z AA BB 0 1_/0 le6 10 10 / 10 I0 /0 /0 2 16 /0 to0 /0 3 /lb to ID 4 A) 10 1 /0 6 /0 Jo /o /0 7 10to /0 Id A -Pan weight (mg)

iý)

O,' \\" '~ B - Pan +Larvae weight (mg) am'~ ?kýl ~Iq X-14 Larvae weight (mg) - A-B 1 1.t6 0 i i Calculations and data reviewed: AJ .t Comments: Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/60014-91/002, Method 1000.0) Species: Pinephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, UV Treated October 8-14, 2002 Project number: 483 OutLfall 101" Dunnett's MSD value: PMSD:

Intake, Dunnett's MSD value:

PMSD: Revdwed byr ,/- 0.1662 14.6 0.1175 104 MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the comnrl and treasnert that can be declared statistically significant in a whole effluent toxicity test. On average, a significant differnce occurs for Environments! Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19 6% from the control (detetmined through Lower PMSD bound determined by USEPA (10th percentile) -9 4%. Upper PMSD bound determined by USEPA (90th percentile) - 35%, The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Piunephalca growth in chronic reference toxicant tests USEPA 2000 Understanding and Accounting for Method Vanability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OI1 Client: Test dates: Concentration (%) Replicate Init almumberor Fonal naumber oflarva A - Pan weight i - Pan + Larva. Larva.wvight(mg) Weight /ltialub Meanlarval Meam i! *(mg)

C o

? romed"Iranir lare (mg) weight (mg) -A-Bf ltarva ie g) (%) v'bdm (%) (%) A 10 10 15187 27190 12003 12003 Control B 10 10 14964 26910 11946 1 1946 100.0 1.1346 8.7 Not appicable C 10 10 14945 26480 11535 I 1535 D 10 10 14871 24770 9899 09899 1 E 10 10 14873 24690 9817 09817 1098% F 10 9 15106 25.110 10004 10004 97.5 0.9840 1.9 13.3 G 10 10 14922 24870 9948 09948 I 10 10 15008 24600 9592 09592 I 10 10 15 145 26630 11485 1.1485 22% J 10 10 15063 26620 11557 1.1557 97.5 1.0454 14.0 7.9 K 10 10 15162 25510 10348 10348 L 10 9 15.175 23 600 8425 08425 M 10 10 15051 26960 11909 1 1909 439% N 10 10 14976 25880 10904 10904 1000 1.1023 6.2

2.

0 10 10 15 156 25410 10254 10254 _P 10 10 15076 26100 11024 1 1024 Q 10 10 15 182 26910 11.728 1 1728 72% R 10 10 14992 25330 10338 10338 100.0 1.0848 11.0 4.4 S 10 10 15073 24470 9397 09397 21 10 10 14980 26910 11930 1.1930 U 10 10 14647 26150 11.503 I 1503 100% V 10 10 15185 25990 10805 10805 1000 1.1555 7.0

1.

W 10 10 15 152 27.850 12698 12698 X 10 10 14645 25860 11215 1 1215 Y 10 10 14760 26610 11850 1 1850 100% Intake Z 10 10 15019 26190 11 171 11171 100.0 1.0960 6.4 3.4 AA 10 10 15 193 25.710 10517 1 0517 LBB I0 10 15 120 25420 10300 10300 Environmental Testing Solutions, LLC Statistical Analyses L ME Oik e wia Sarvlva T0u6.7 DyGe SM Dam M 10V2 Teat D* PpFX-Smmpla 0: VA. Saquaya Nue Plaft EndDaft 20(134) Lab W* Es-Ev. Tes~tig Solutions 31112001pe; DMR-Dhcha. Motisortag Report Smpla Daw PrI-loed. SlAP 91-UAPrwibwaw Tat Spede. W-Thnqtai Pe-e sC o-mmn 2 W-mated Caeo.% 2 34 D -c~mw w 1121)1,10-3 D~aal .203 11946 2.2535 0.9899 10.9l 09817 2.0004 0.948 0.9592 22 1 1485 1.1557 1.0345 0.3425 43.9 1.1909 1.0904 1.0254 1.1024 72 1.1728 1.0338 0.9397 1.1930 100 1.1503 2.0805 1.269" 1.1215 T U em'aurd I-Tined amaie Come.% Mea N-M4m Mean M Max CV% N 1-S"tt Cikdti2 MSD Mem N-Mat D-Coaol 1 1346 1.000 1.2346 09899 1.2=3 1.697 4 11346 2.0030. 1098 09840 08673 09840 09592 1.0004 !.81 4 2.183 2.410 01662 b.0744 0.9470 22 10454 0.9214 1.0454 0.8425 1.1557 13 980 4 1.294 2.410 0.1662 1.0744 0.9470 43.9 1.1023 09715 1 2023 1.0254 21909 6176 4 0.468 2.410 0 1662 1.0744 09470 72 10848 0.9562 1.0848 0.9397 1.1930 212.050 4 0721 2410 0 1662 10744 09470 100 1.1555 1.0185 21555 10805 1.2698 7043 4 -0.304 2.410 01662 1 0744 09470 ASuilsary Tests StatiC CritEni Skew Knrt SbaPUDWlek's Test indhcates normal disnm buton (p 0 1) 0.937664628 0.884 -062160028 000331763 1 Bindeis Test ioditcates equal vannces (p -80 13) 2444832802 1508631706 Itypotdeald Test (1-nhi. 005) NOEC WOEC ChV TU MSTh MSDp MSB MSE F-Prob df Duoenres Test 10o >100 1 0 16618429 0146472723 0015595262 0009509897 0.200275555 5.18 inear Interpolado, (* 0 Resampkes) Point SD 95% CL(Exp) Skew 1C05* 10.352 ICI0 >100 MCIS >100 10 IC20 >100 IC25 >200

09.

IC40 >100 0.8: IC50 >l00 0.7 tndtates IC esumnae less th-n the lowest concesa"ron 06 ' os0 o04 Wr 0.3 0.2 01 00 o- - oo. -01 0 20 40 60 80 100 120 Dose % Dose-Retspose Plot 14 1-tail. 0 05 level

08.

'of sgnificance 2 s 06 04 0.2 0 0 sqn_lO-OS-O2data.xls Environmental Testing Solutions, LLC Statistical Analyses Larval Fbh Growh sand Survival Tes,-7 Day Growth Stat Date: IM102 Test D PpFRCR Sample ID-TVA. Sequoyah Nuclear Plato. Intake End Dowe-10/15M2 Lab 1D-ETS-Env. Testing Solutom Sample Type-DMR-Ducharge Montorring Report Sample Date: ProtocoL EPAF 9 I-EPA Freshwater Test Species PP-Pamephala promelas Comments U-Treated Coa-%/. 1 2 3 4 D-Constol 1.2003 1.1946 1 1535 09899 100 21850 1 1171 10517 I 0300 Transform: UIomusforied 2-Tailed Conc-% Mean N-Mean Mean Mm M" CV% N t-Stat Critical MSD D-Contmrl 11346 10000 1 1346 09899 1.2003 8697 4 I00 10960 09660 10960 10300 1 1850 6384 4 0639 1.943 01175 Auxiliary Tests Sttistic Critical Skew Knrt Shapiro-Wlkis Test indicates normal distrbuon (p 0 0) 0924149632 0749 .0 83582504 .0 054987614 F-Test mdwca*t equal vaiances (p - 0.59) 1989268303 4746834564 Hypothesis Test (1-taul 0 05) Homoscedasuc t Test indicates no significant differences sqnlO-08-02data.xfs Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0) Species: Pimephalespromelas Daily Chemical Analyses Client: Seqi Test dates: Octc Project number: 483 uoyah Nuclear Plant Non-Treated ober 08-15, 2002 Reviewed by: Cs' Initial Final Initial inal Initial Final Initial Final Initial Finl Initial Final Initial Final pil (SU) 7.2 7.32 7.1 72 .2 75 .7 7.421 78 .0 78 77 .6 77 DO (mg/L) 78 71 81 69 8.4 7.2 8.1 69 7.8 6.9 7.8 7.6 7.9 7.7 Conductivity (pmhos/cm) 294 302 301 3 34 Control Alkalinity (mg/L CaCq) 662 60 Hardness (mg/L CaCOs) 82 Temperature (C) 24.4 24.81 242 250 246 24.9 246 25.3 24.3 25.0 24.7 24.8 24.6 25.3 PlH (SU) 784 7.31 783 7.20 7.86 741 794 7.361 7.90 7.40 7.89 7.78 7.82 7.70 10.98% DO (mg/L) 78 71 82 69 8.2 7.2 80 6.9 80 69 7.8 7.7 80 7.7 Conductivity (Itmhos/cm) 283 288 301 294 290 293 302 Temperature (C) 244 248 245 250 245 24.9 24.7 25.3 24.4 25.0 24.7 24.8 24.7 25.3 PH (511) 7 83 7.33 7 82 7.24 7.84 7.40 7.93 7.33 7.87 7.43 7.87 7.74 7.83 7 69 22% DO (mg/L) 78 70 82 6.8 82 6 11 68 80 6.6 7.9 80 Conductivity (ftmhos/cm) 270 271 286 284 277 284 288 Temperature (C) 246 248 24.5 25.0 24 5 24 9 24.7 3 24 4

2.

24.7 24.8 248 25.3 pH (SU) 780 7.33 780 7.17 7.81 7.36 7.90 2 7.83 7.40 7.82 7.76 7.83 7.72 DO(mg/L) 79 70 82 68 8.3 65 8.1 9 80 6.5 7.9 7.7 8.0 7.7 Conductivity (lamhos/cm) 248 247 261 259 254 256 Temperature (C) 246 248 24.7 25.0 24.7 249 24.7 3 245 24.7 24.8 24.8 25.3 I O1 I.I01 1.1 -1. "iD 7 851 7.30 7 7RI 7V*t DO (mg/L) 7.9 7.1 8.2 6.8 83 65 8.* 80 7.9 7.6 8.1 7.7 Conductivity timhos/cm) 214] 215 224 222 220 222 226 Temnerature PC'C H11 (SU) DO (mw/L) Conductivity (;jmhos/cm) Alkalinity (mg/L CaCOQ) Hardness (mg/L CaCO) I-I- Total Residual Chlorine (mgm Temperature (C) nil (SU) DO (mg/L) Conductivity (1lmhos/cm) Alkalinity (mg/L CaCO3) Hardness (mg/L CaCO1) Temperature (C) Sr4 24.7 24.8 249 250 247 24.9 24.7 25.3 24.5 25.0 24.7 24.8 24.8 25.3 7.70 7.28 7.74 7.19 7.72 7-34 7.90 7.30 7.68 7.36 7.66 7.74 7.78 7.71 80 7.2 8.2 6.9 83 64 8.1 69 8.1 6.4 7.9 7.6 8.1 7.8 182 192 187 188 61 631 62 72 740 100 ý0. 10 <01 <0.10 25.01 24 8 25 0 25 0 24.71 24.91 24.71 25.3 24.51 2:5.01 24.81 24.81 24.81 2255.33 7 741 7371 7.731 7.21 7.421 7.381 7.841 7.341 7.60 7.361 7.65 7.39 7.72 7.81 8 2& 7.2 831 6.7 A 8 4 7.21 8.0

6.

8.11 641 8.01 801 8.1 80 62 62 5 86 80 8

20. 10

<0 10 <01 25.21 24.81 2491 2500 241 24.91 24.71 25.3 r 24.41 25.01 24.71 24.81 2461 2S.3 D" (3Ui 72% 100% 100% Intake Total Residual Chlorine (mglA S. .a Temperature (*C) 7.32£ 7.38 7771 '7'7"41 "/ RNI qqa kp5 at Species: Ceriodaphnia dubia Client: 7j7,- -- ewW, Date: /~~p Fullstrength Chemistry:- Oawt-ýtL /101 Parameter SampeNumber: ControlBatch: 1___ 2 3 7-2 ýio-j1011-CL-(S.U.) 1.1?- DO e0 R ý ConductivityI8. (mos/cm 1af)7 Alkainity (mg CaCO]L) (A0~)~ 2. Hardness W/D (mg CaCO3IL 0 Chlorine /ao -013.,.c Collection start date 0L(w 00O 0-c07 Collection end time )(LaI oqol Z5 Grab or Composite 37"%ie (duration)Ci4~ of~ Temperature CC) 03* zq upon receip zt C Physical pale. emAL. PALe characteristics -tn T(3 -AtI. Cl~f r LeA VL. cLeALe Dates sample used /oq.,e-dz ,/0-10-01 /O.I/Z.Iz. /o.oq.oz. /0.11. /64. IS. 1 //0. I4-. t)Z ETS Project and 4143 Sample numbers 102io00.041 LUZIOI. 0 O2.IoI2.0(f gZ9 1.21 7.5 (e -iS (oO rugeooi Species: Ceriodaphnia dubia Client: T~ Full-strength Chemistry: D-ZittDe. Date: 110-6B-62 Parameter S~ampleNumber: I Control Batch: nH I (S.U.) DO (mg/L) -7.q 1 .5. i EAL 'M(OO 1.2 t

1

.5 1 Conductivity (a.mhos/cm) / E3 L93 Allkalinity (2 (mg CaCO3/L) Hardness (mg CaCOJ/L) go0

92.

Chlorine401 /01 e,1( Collection start date 1.(.- 0OO 01-2 Collection end time 00( Lc,ý 2 Grab or Composite ýAuý &wz (duration) C&O- -CRO oK~ Temiperature (QC 0.3C 0.9-Cc. upon receipt Physical ploa.A PA P-Le characteristics -r -Aa TAW~ ctvaL CLAiL cLeAoL Dates sample used 10-610 t/o o . /Z z~o ETS Project and 14 Sample numbers lotosg-od.06 2.Io.CISFOZIOIZ. 08 -1 7 I.R IL 1~L 30 1 (A 962

Mý 1.1 JiLl

(.0 92-R Page 7 of 8 Species: Ceriodaphnia dubia Client: - VIA - gf1 Date: 6, 06-.02 Species: Ceriodaphnia dubia Date: //).6 1qA ton Parameter3456

NTROL, PH (S.U.)

4qlIZ a44.o DO (m/L 6.1 Conductivity ,~~.qo30 Codutiit

Temperature i

DO m/)

f3.0 j3.0 -+. 0.0 9z/. Conductivity Temperatur~eAz. i.~z. Q

2.

pH (S.U.) 9*qo /O Conductivity 2 -Temperature 2- -z52 zq, -5 2q.1' 2/7,q.o(o ~Conductivity ) lai (pmhos/cm) Z(0H 2/3'4 Temperature~I -niia qI -q q 2-.-- ~ l.n I ~a i I Cniu )ia T~iI I in Initi 17;n T.;#..] InIn Yin I Ini a] Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses Sequoyah Nuclear Plant Non-Treated Client: Test dates: October 08-15, 2002 Project number: 483 Reviewed by: Ctl(- Concentration Parameter Da 0 Da I Day 2 Da 3 Da 4 Da 5 Da 6 Initial Final initial Fin Initial Final Initial Final Initial Fin Initial Final Initil Final pH (SU) 7 82 782 7.71 7.89 7.92 800 7.97 8 00 787 !7.91 7.84 8.0 DO(mg/L)

78.

8.2 801 84 8.4 8.1 81 83 7.8 7.7 78 Conductivity (pmhos/cm) 294 302 314 312 301 304 Control Alkalinity (mg/L CaCOQ) 61 62 Hardness (mg/L CaCQ) 82 Temperature CC) 244 24.9 243 25 3 24.5 25.0 245 25 2 245 24.9 246 P (SU)84 7.75 7.83 7 87 786 802 7.94 807 7.90 7.92 7.890 8.10 DO78 80 82 84 82

8.

8.0 8.3 80 7.7 7.8 10.98% Conductivity (limhos/cm) 283 288 301 294 290 293 Temperature CC) 245 249 24.4 25 3 246 250 24.7 25.2 24.5 249 246 2 Pll (SU) 7.83 776 7.82 7.89 7.84 800 7.93 806 7.87 7.91 7.87 1 DO (mg/L) 7.8 80 82 84 82 8.1 81 83 80 7.8 7.98 22% Conductivity (pmhos/cm) 270 271 286 284 2 284 Temperature CC) 247 249 24.4 25.3 246 250 24.7 25 2 245 24.9 24.6 25.0 PlH (SU) 7 80 7.74 780 7.90 781 802 7.90 805 7.83 7.91 7.82 8.15 DO (mg/L) 7.9 80 82 84 8.3 82 81 8.3 80 7.9 7.9 8.3 Conductivity (pmhos/cm) 248 259 254 256 Temperature CC) 247 249 246 25 3 247 250 24.7 25.2 245 249 246 25.0 pil (SU) 7 62 7.71 7.76 7.91 7.76 8.00 785 805 7.78 7.94 7.77 8.13 DO (m L) 79 80 82 84 83 82 8.1 84 80 7.9 7.9 Conductivity (pmhos/cm) 214 215 224 222 220 222 Temperature CC) 249 24.9 247 25.3 248 25.0 24.7 25 246 24.9 24.7 250 PlH (SU) 7.70 7.76 7.74 7.87 7.72 7.97 7.90 803 7.68 7.89 766 8.10 DO (mg/L) 80 80 82 85 8.3 8.2 81 84 8.1 8.1 7.9 84 Conductivity (timhos/cm) 182 183 192 1 187 188 100% Alkalinity (mg/L CaCQ) 61 63 62 Hardness (mgIL CaCQ) 72 74 100 Total Residual Chlorine (mPIL) <0 10 <0 10 <0.10 Temperature eC) 253 249 24.91 25.3 248 25.0 24.7 25.2 246 249 24.7 25.0 piU (SU) 7 74 7.75 7.73 7.86 742 7.91 784 7.96 7.60 7.87 7.65 807 DO (mgL) 821 831 831 84 8.41 8.11 80 80 8.1 8.0 8.0 -4 8.1 Conductivity (pmhos/cm) 181 195 190 183 85 100% Intake Alkalinity (mg/LCaCq) 62 62 Hardness (mg/L CaCQ) 86 80 Total Residual Chlorine (mWL) <0 10 <0 10 Temperature CC) 252 24.9 248 25 3 2471 250 24.7 25.2 246 24.9 24.7 25.0 Species: Pimephales promelas Client: 1Ff?,- JfJwQQUAI~ Date: Ia. m6oz Full-strength Chemistry: &)ffdJ- /01 Parameter S mple Number: Control Batch: 1 2 3 16.6'-61 ,O-L lol-c2-* pH g e- .( p(S.U.) T.*o q.8? 7 -7.16 DO

8. C.)

R.[ (mgfL)__ Conductivity IiL 112- -q'7 2qq

AO (imhos/cm)

Alkalinity (mg CaCO 3/L) (, (,2 (- C,2. (.0 Hardness (mrn* CaCO2 LU -7 100 V(. ?' 2. -S C hlorine 1 0 06. 10 /,.O, 0C (M91L) Collection start date I-0d-O* I-o-C Collection end time Grab or Composite PoUfl.. (duration) r coAposie. C APosf. Temperature (0C) 0.3*C 2.4 "C, upon receipt Physical P4LL eAtP PA Le characteristics I-tw. Dates sample used /0.06,1 Z 1.Io.02 IO./0 z.I to.1.Ol 02 .1367Z ETS Project and 46 3 01 Sample numbers OZ1v.O6.q 0210:0.03 o2I l i II Species: Pimep hales promelas Client: -i-lA-6texiph ,f Date: MO-4O6-6 INJoTrSATetý Full-strength Chemisly: J;JV Parameter S ple I uber: Control Batch: 1 2 3 1io-bC4 1 0200- ,o'-11-67 I pH q -qz 1.(0O -781 ( (S.U.) I___ I_ DO 6-8 (Mg/L)6._ Conductivityt Z5 q 301q (gmhos/cm,)__ (mg CaCOfL 627 140 Hardness (miz CaCO3JL) 'a &Za Chlorine z(.o 0 -0).10 10.I Collection start date 10.0(0,02 (0- Da.L 10- IQ-o-z. Collection end time o t Grab or Composite Rhut -1%3R-Wkw Temperature (TC) 0*- 24C upon receipt r____ Physical 19L~ F'ALe-. L characteristics VL, F&3 m cLtawn cLeAe... cUe,. Dates sample used l'-6.t / -1,o (0Z 1412.oZ ETS Project and 45 Sample numbers O02J408.D51 oa_10,.03 ovziZ.Qý Species: Pimephales promelas Client: 7"0 ~ I~o NTA-.eb-r* Date: /6.66-6Z Page 7 ot Species: Pime~phales promelas Client: -rVd.-.5e400uaJ Date: A-46d.42 Da Concentration Parameter 3 4 15 16 CONTROL pH (S.U.) 04.8.141 W:1 1 (A .4 Conductivity 31 30 0q.1 Temfperatur~e -Zq ýZ ( (0 2* -3 25.6 zq.-7 z'Iu zq zSs PH (S.U. -44 .8q 1-.16 DO (mg/) 0(0,9. -. 8 1 3 /0. q6'/. Conductivity Temperature 2 q.1 70f~4 Z-eD -ztq --7 ZLI.-1 Z-z.3 PH (S.U) 3 DO (mg/L) (M ./ Conductivity Temperature -ZLI. -7 Z~ (0c) PH (S.U.) -. le$V(J 3§ ~ ~ '2 DO (mgL 81C.0 .8.0 1 439 */ Conductivity Z~ Temperature V4l.1 ?S.-3 2q. -Ozq. 1 VI-zq.' F .~ PH___ (S.U. 3-P6 4

4. 8

-4.:;<.0 3-~ TO ((mg(/L6.8.3 ,z -/. Conductivity Temperature .32. 9

z.

2 d 3 PH(S.U.) 344 13.-'1 DO (mg/L) (q1 /0. Conductivity 13i)I9 (gmhos/cm) 11 Temperature Zq.3 S, q2-2Y.00 Z PH (S.U.) =e1.54 -4.140-Ao -+_5 Conductivity 0

9.

61 job'* (prnhqs/cm) 03~Z 19//1 Temperature 7c7 Z. e'I

2.

/7~I~/ (-C) IUILA4tAl~ I.' ILaU U

'~~I I I-' NomoteAreA**,ý

Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0) Species: Pinephales promelas Daily Chemical Analyses Sequoyah Nuclear Plant UV-Treated October 08-15, 2002 Project number: 483 Reviewed by: CL.- Concentration Parameter Da 0 Da I Da 2 Da 3 Da;4 Da 5 Da 6 Initial Final Initial Initial Final Initial Final Initial Final Initial Final Initial Pl (SU) 785 744 7 83 7 784 753 789 741 789 7.30 7.77 7.31 7.92 DO (mg/L) 80 73 80 70 85 71 8.11 7.11 7.71 64 7.7 7.2 807. Conductivity (pmhos/cm) 291 291 302 296 301 313 Control Alkalinity (mg/L CaC6) 60 Hardness (mg/L CaCQ) 82.82824 _______Temperature (C) 2451 25 1 246 25.1 244 247 244 252 244 24.9 24.7 24.7 24.6 2521 ni (SU) 785 740 780 722 782 757 789 742 788 7.34 7.73 7.29 7.90 768 10.98% DO (mgL) 80 73 81 70 85 7.1 82 70 7.7 6.4 7.7 7.1 7.8 7.8 Conductivity (Itmhos/cm) 284 285 297 295 290 295 306 Temperature CC) 246 25.1 247 25.1 245 24.7 245 25.2 245 24.9 248 24.7 249 25.2 P! (SU) 784 742 7.76 7.22 783 7.52 789 7.38 7.86 7.34 7.72 7.28 783 765 DO (mgL) 80 74 81 69 85 82 68 7.7 66 7.8 6.9 7.8 7.9. 22% Conductivity (pmhos/cm) 270 270 284 281 275 282 297 Temperature CC) 246 251 248 25.1 245 245 252 24.5 248 24.7 24.9 252 11 (SU) 7827 79 7 23788 7.36 7.8 4 7.67 7.28 7.87 763 DO (m2L) 80 82 685 82 7.7 7.7 7.8 7.8 Conductivity (pmhos/cm) 248 246 260 256 252 257 277 Temperature CC) 24.7 24 8 25.1 246 24.5 24.5 24.7 24.8 25.2 PH (SU) 7.80 73 7.76 7.29 7.79 75 7.86 73 7.81 7.24 7.65 7.6 784 7 621 7.36 7.7 7.2 79 7..6 7.2 72% DO (mgL) 80 82 69 85 81 76 7.7 7.9 7.7 Conductivity (prmhos/cm) 214 215 22 219 224 237 Temperature C24.7 25.1 24.9 25.14 24.6 24.7 248 25.2 245 24.9 24.7 24.7 248 25.2 pH (SU) 7 591 7.341 7.72 76 7.73 7.471 7 82 7.37 7.77 7.20 7.59 7.25 7.79 7.63 DO(mWL) 80 74 82 84 70 80 66 76 65 7.7 68 80 7.7 Conductivity (gmhos/cm) 182 190 187 200 100% Alkalinity (mgIL CaCq) 6163 62 Hardness (mgIL CaCQ) 72 100 T otal R esidual C hlorine (m ,/L <0 10 10 <0 10 Temperature CC) 249 251 25.1 25.1 246 24.7 248 25.2 24.5 24.9 24.8 24.7 24.9 25.2 pH(SU) 771 741 7.72 7.20 7.72 7.53 7.78 7.74 7.24 7.55 7.33 7.66 DO (mWL) 81 7.3 81 7 84 70 79 7.8 66 7.7 6.6 8.0 Conductivity (pmhos/cm) 180 180 186 183 16 100% Intake Alkalinity (mg/L CaCq) 62 6 Hardness (mg/L CaCQ) 886180 Total Residual Chlorine (mL <0 1<0 10 _________Temperature (C) 2541 25 11 _ 25 2 25 1 2461 24.71 246

2.

24.51 24.91 _ 24.81 24.71 248 22 Client: Test dates: .... *4 1 T i h I I UI-v nininA~~a %-a&Lp Mr Species: Pimephales promelas client: "-, UI LdtAtioC Full-strength Chemistry: Ck4AtL /6 / Date: /.d -h Z Parameter Sam le Number: Control Batch: 1 2 3 jp.(4.i2 ,o.Dq4J. 16-1(- qo7 pH -8 -%A112 (S.U.) Do S.0 8.] 7., eo 8.0 Conductivity in lqz-Ir7 ze1 (uosIcm) I __ Alkalinity 6S ( 2.0 (rag CaCO3/L) Hardness (mcr CaCO.IU 100 Chlorine L..(21C, 0.,0 (mg/L) Collection start date iO.O1.o-2 IO-Of-O-L fo-10.OZ. Collection end time o,. Grab or Composite --hoopa 0*.. (duration) co c"Mtare coataSaI Temperature (C) Z.4vc upon receipt 0_._r-__ Physical (f4LL1 t f-?LC Ae characteristics f-n, "T'A 71W Dates sample used I.. g.OZ I/.lo1Z 10.IZ.oz IO. 9.0 z IL).l1,OZ /0.13-.2 ETS Project and 48.6 Sample numbers 0Z100.d OtIOO.03 011O1.61 StJJL 0 Species: Pimephales promelas Client: i t/A - .'*p ,o, gh F %y Fuf-trngh h-ity:;-zl Date: 10-46.64 Parameter Sample Number: Control Batch: 1 2 3 /6.4-74 Z O. Oq,0. I02 fl-o2 pH _s.u.) -11 -7. -7.-7 (So. 1..I 7.72 -i.i'

8.

____o. DO

8.

(mg/L) Conductivity (gnos/cm) (Mg CaCOO 3 L) 51 z 40 Hardness Ro 912 if 2-Chlorine ý.O. 10 (M-/L) Collection start date 1O.O 0,OZ 0.08.o0 /o40-o2.. Collection end time Grab or Composite -o ou (duration) C Aqoe ctM' Colre Temperature CC) Q-3C

0.

"C L4 "c upon receipt L_3C__41_ Physical p a.L PA,-L PALe characteristics "/", T "IJ -r ; Dates sample used /o.08.OZ /0./06.Z /0./1.b2 /o.N'.oZ ETS Project and I183 Sample numbers OZ7.IO.OT 0 7 oz/O12.OJ Species: Pimep hales promelas Client: ii~-774ýwjo LJGJEV Lnenusirv: Date: /b-gd.2_ Concentration Parameter012 CONTROL pH (S.U.1.83145 Do &gL) C .3 6.0 10 Conductivity Temperature 2... -c 4 qZ 74.;q ~ v - L g/!L) R.0 -4. Ic. 98) Conductivity Temperature zq.

25.

zq. zq. DO mg/) X0 a

1.

8.

(PAq (C 5 2 2'1 Conductivity (Wmhos/cm) 2402IS Temperature -25. zq.I k..L Z51 _(__ CC) z pH (S.U.) 7.2. 0 AC DO (mg/L) -4.3 (0. 6' .5:, 72, 1 Conductivity (mos/cm) ZIL3Iz o Temperature 27z(~ pH(S.U.) .8 q1-( -4.( 4 DO (mg/L) .0 1 1..(o. IL) 0 ~. Conductivity -(Wnos/cm)Z6Z6 Temperature q z( pH (S.U.) n-.' I ~/k.DO (mg/L) R.1Q -Conductivity e /66'/ (Wunhos/cm) /9 Temperature 7q.~ '/ CC) 24k21-initil Fin Iitial II Final I Initial I 1' Kal 1 wi 0 rfoI I &JWAULIUU39Page t; ot s5 Species: Rimephalespromdlas Client: 7V Date: /0 - j 5-4 Environmental Testing Solutions, LLC Alkalinity (EPA Method 310.1) Matridx Water, MDL = 1.0 mg CaCO3/L Analyst Date analzed )b.1 Titrate samples to pH = 4.50 S.U. r* normand mu tl, dermidnaon: pH of Normality Normality (N) of H 2 SO4 pH Factor or Multiplier Deionlzed Titrant check Begin End Total (5 ml NaaCOs 0.05)IE -(Nx 50000)I100 ml sample water reference standard ml nIl ml - 0.25/E - N x500 - 4.5 S.U. number number I (E) (acceptable range - 0.018 - 0.022) eIw-LIA. 0,0. O.b -o.3.-&I Laboratory control standar& Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 standard number (TM) volume Begin End Total Multiplier (-tg CaCOWL) (acceptable range (mg CaCOVL) (m) mi ml ml I 90to110%) IORU 100 100 4 c9 10 O.c qC'A 1.,q Duplicate le precision: Sample Alkalinity %RPD i Sample Sample ID volume Begin End Total Multiplier (ing CaCO/L) ((S - D) I[(S+D)/2]1 z 100 number (ml) ml ml ml (acceptable range - 4.10%) Io0.1iO j S VOo 22.-q Zj.z _5,.1-._ S (OZ. )D.DZ.oz-IupiCal" to zi 0. D ((UI Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A). standard number A (SV) volume Begin End Total Multiplier (mg CaCOdL) (m9 CaCO3/L) (ml) ml ml ml I AO(050 oo 5i0z. 1(. 11.3 19A.' IW Sample alkalinity (B) Measured spike value (MV) % R = MVI SV x 100 (ng CaCOIL) MV = A - B (acceptable range (mg CaCOJL) -75 to 125%) 6O1 159 116,1 Sample measurements:._____ Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (me CaCO3JL) 10-0).02 M/7W Lls 4to" tP -, q-,. CIA (0* z, I t.0, 1-2 (0-2 0 0-O7.OZ/J ";.z zaq (.-Z ID.b.oZ R(0 I35.e ;P3 (o2 O. J).O Z40.o 32,1 (,.

10. L3.o&

32.1 3e.i

,.3 1 Ob..O'Z. I Pizo 36-*.o Iq.'9 3-5o's

).~ 2 0~ ~~ J 0 04W 3 0.0 1o zOj 0.5 0 Date reviewed: I /o'-ZI,2... Page (0 pare I -of Reviewed by: I A[ I 6/1

Environmental Testing Solutionq. TT, P.age Q ofj, Alkalinity (EPA Method 310.1)

Matrix: Water, MDL = 1.0 mg CaCO3/L Titrate samples to pH = 4.50 S.U. Date analyedI Laboratory control standard.. Reference True value Sample Alkalinity (MV) % RS =M V / TV x 100 standard number (TV) volume Begin End Total Multiplier (zng CaCOCL) (acceptabe ran0 e (ing C"CO3 L) metml ml ml 90to110%) ate100 1 100 IqI Z4.Oir q. SampleAlkalinity %RPD= SampleBegin End Total Multiplier (mg Caco3IL) ((S - D) /[(S+B)/21) x 100 number (ml ml ml (acceptahl-range - ;: 10%) OM0o0-2 SW-3 100zi~ Z'iA3 3K. (o.q sr-Matrix pike recovery: Reference I Spike value I Samnle I I I I I standard number (SV) volume Begin End Total Multiplier (mg CaCO31I) __!*Cao0 I 5 I '71,.,I I.,_ I II (Me 9aC-O,/L)- (m]) ml ml ml Sample alkalinity (B) Measured spike value (MV) % R=MV/ SVx 100 ( -9 CaCOL) MV-=-A-B (acceptable rang-e (me CaCO3/L) 75 to 125%) Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID ml ml ml ml Multiplier (ag COL) ov 0 t*.o( 6C,.irlt, Ic )o L.I I.z q a .'7

4.

23

.3 13.*,

Li.3 O.I bo12.09 3

0.

+

O.bZOa3 z(,.. 3Z .86 3k

-4 W.

v.

0-0 5 .8 ' jqO 61640___ -6, I____. iq jo Reviewed by: D v IC k .age e8* Date reviewed: D *a--O.I I Environmental Testinu Snlitinrn *.T (" a.... Page of_ 9 Eniomna ti -o~in ir Jr'~ Cae4~~ Analyst f.ýo Date analyzed 14).Iq _or Total Hardness (EPA Method 130.2) Matrix. Water, MDL -1.0 mg CaCO3/L 2 t Tifant.ormaU and multiplie detennination: Tltrant Normality check Begin End Total Normadity (N) of EDTA pH Factor or Multiplier reference standard ml ml ml -0.2/E =(Nx 5000)50 ml sample number number (E) (acceptable range - 0.018 -0.022) -N 1000 le INRO*b INWO410 1 *,O 10.0 110.0 O.01 ZO Matrix spike recovery: Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3/L) m(r CaCO*1 L) (ml) ml ml ml INusxo ,to. Zo 150 Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCOIL) MV = A - B (acceptable range (MR CaCO,&) = 75 to 125%) qo -40 -O* Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rag CaCO3IL) (sldb -0gCCoa) 6DV-0,0 0.0 0?D ,r me q'.3 1 40.2.0*-2 IM0 -0., qO.4., _______z 4__4.5_ ý. Jo. 13.oz. _0.0 q_._ 0 1.os.06.02 SSUioJ 10o q ._ U qq ozeOZ100P-01 G1 (- 66 Note: If >lSml of titra= is used, sample must be diluted. Date reviewed 2-1 iftie Reviewed by: Environmental Testine Solutions. LLC Analyst [

j.

Date analyzed OLAIiV II, Page 8q Paae of Total Hardness (EPA Method 130.2) Matrix: Water, MDL - 1.0 mg CaCO3/L Duplicate sam precision: Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg Caco,/L) ((S - D) /[(S+Dy2]} x 100 number (ml) ml ml ml (acceptable range - :10%) O .o. 5,* O S 41 _01 3._ _O_ Dplicate D Matrbc spike recover: Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (Mg CaCO3IL) (mg CaCO3/L) (m) ml ml ml IASNO) 40 606 S.-4 l" .6.1B 20 =It-o0 Sample hardness (B) Measured spike value (MV) % R =MV /SV x100I (mg CaCO3/L) MV = A - B (acceptable range (11g CaCO3 IL) -75 to 125%) -:ýU L40 Sampe maurements:- Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO /L) (should be - 0 mg CaCO31L) 621(010.0 ON it,. 0! 7-50 1 1.- i's. - 7C. -4 LI o07112.0-1 3 1i.2L 20.7-O [Do00 ,OZipod. Os N a Ntj I =Z_ q-V15w. 1171 o110.03 Z____ ^s.Q C.O 80 o21*.oS .3 _S_0 o 3o 1..1 0'ooo 2110-0L VACFP R 10 0.'4

e4

".1LII ot 8-S5 I. 1 - ________0 Note:~~ 1f LL~m o4 &2tan is used 1apl must be diue.RveeIy at eiwd o Date reviewed 1 10-2m-O Z-I Revicived by: 41 L./ I Note: If >l1rnl of titran is used, sample must be chluted. 7.Envirommental Testing Solutions, LLC Page qs Page I.... of/ Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter Accumet Model 25 pH/Ion Meter A n a ly st, Ut Dateazw lOO,oz. I Acid reagent: Slope: D 4z-Ooo5 Note: All samples were analyzed in excess of EPA recommended Reviewed by Date reviewed [u12ý Z-

Environmental Testing Solutions, LLC Page S1 Page -_2 of Total Residual Chlorine (EPA Method 330.5)

Matrix: Water, MDL = 0.10 mg/L Meter Accuwet Model 25 pH/Ion Meter Date analyzed L0. Ii.II Iodide reagent: Acid reagent: Slope: Calibration: 0.10 mg/L 1.00 mg/L Reference standard number = ad5*y* I Note: For samples with a residual chlorine of > 1.0 moL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard. Reference standard True value (TV) Measured value (MV) % RS = MV/TVx 100 number (MVPL) (rag/L) (acceptable range - 90 to 110%) I A<S C)10.50 03( )bo.67/ Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD =- (S -1D)/[(S+D)/2]} x 100 number (ag/L) (wmptabie range - 10%) OZ.101 OlO. AflctiJ'bofCO Z-xO 1 7 Duplicate -Dg..05LeZ Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (m* Blank (shouldbe=OlOmgL) e = <Oo.10 6z Ioto oa -qIhBh WLULCP ona o, o.oq--q 8210lo.o0. 1A -.*c* - )j a x W.ooSez otio.03 TIR-SON)- )MTrO w S..0 Z .Yn m ,rc~ ncnPa% nolazng ume (,15 minutes) unless otherwmse noted. OZt)Lorato ry /9 control sta*ndarLrd:Lý Laboratory control standard:. 4o.-Oo 2 Reference standard True value (TV) Measured value (MV) % RS = MV/TVx 100 number (raQL) (mg/L) (acceptable range - 90 to 110%) 0.50 0.qO 07.j Reviewed by I Date reviewed Environmental Testing Solutions, LLC Page __Cie Page......of 4.I Total Residual Chlorine (EPA Method 330.5) Matrix-Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Date analyzed 1 I1-1.,7-1 Iodide reagent Acid reagent: Slope: Calibration: ~~ ~0.10 = mgI.ILOMWL I "Reference standard n jumber 1_ 4)o I. Note: For samples with a residual chlor*e of > 1.0 mg/l, the cahl*tion range must be adjusted to bracket the chlorine levels ofthe sample&. Laboratory control standard: Reference standard True value Measured value (MV) % RS = MV / TV x 100 number (mva/L) (M L) (acceptable range - 90 to 110%) J S oo0.50 &.Q q-.44 Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]} X 100 number (mgL (acceptable range -:h 10%) V Duplicate D 44.00 L#OZ Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number mmrL) R.... Blank (shouldbe=<O0.10rgL) AX, 0 C,,* t* ,,*t bl1203 ThýkXn UX)T P palrWA S11fakth 14 --a 0 0.291 21C1 M.A P -KOr8 1 A-0,,. OZ_ Z0Z.oe 7j -&-zJ Koh o z- -7PL _ooo_,_ Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted. Labordtory control standard: Reference standard True value (TV) Measured value (MV) % RS =MV TV x 100 number (rag/L) (mg/L) (acceptable range - 90 to 110%) SN o0.50 0.63 qZ../. Reviewed by Date reviewed {~ t.

'1 Sequoyah Nuclear Plant Biomonitoring October 08-15, 2002 Appendix D Reference Toxicant Test and Control Chart A'

A Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water Test number Test date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 12-12-01 01-04-02 02-05-02 02-13-02 02-26-02 03-28-02 04-23-02 05-07-02 05-23-02 05-24-02 06-04-02 06-11-02 08-06-02 08-13-02 08-20-02 09-10-02 09-17-02 10-01-02 10-01-02 10-08-02 7-day IC2s CT (g KCI/L) (RIL KCI) 0.57 0.63 0.49 0.61 065 0.56 0.70 0.61 0.76 066 067 0.70 0.55 0.58 0.63 0.61 064 060 0.47 0.53 0.60 0.56 0.58 0.59 0.59 0.60 060 0.62 0.63 063 064 0.63 063 0.63 062 0.63 062 0.62 0.61 S Control Limits CT-2S CT+2S 0.04 0.07 0.06 0.06 0.06 007 0.06 008 0.08 0.07 007 0.07 007 007 007 0.07 006 007 0.07 0.52 0.42 0.45 0.46 0.47 0.46 047 0.46 0.47 0.48 0.49 0.48 048 0.49 049 0.50 0.50 047 0.47 0.69 0.71 0.70 0.72 0.70 0.74 0.73 0.78 0.78 0.78 0.78 0.78 0.77 0.76 0.76 0.76 0.75 0.76 0.76 SA.1o Warning Limits CT - SAJO CT + SA. 0.07 0.07 0.07 007 007 0.07 0.07 0.07 008 0 08 0.08 0.08 0.08 0.08 0.07 0.08 007 0.07 0.07 0.48 0.44 0.46 0.47 0.47 048 048 0.50 0.51 0.51 0.52 0.51 0.51 0.51 0.50 0.51 0.50 0.50 0.49 0.72 0.68 0.70 0.71 0.71 0.72 0.72 0.74 0.75 0.75 0.76 0.75 0.75 0.75 0.74 0.75 0.74 0.74 0.73 SAS25 0.13 0.12 0.12 0.12 0.12 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 0.13 Control Limits CT-Ssm CT + SAm 0.39 0.35 0.37 0.38 0.38 0.39 0.39 0.41 OA2 0A2 0.43 0.42 0.42 0.42 0.41 0A2 0.41 0.41 0A0 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. CT = Central tendency (mean IC25). S = Standard deviation of the IC25 values. SA.o = Standard deviation corresponding to the 1011 percentile CV. S. 10 = 0.12, as determined by USEPA for the method and endpoint. S -5 = Standard deviation corresponding to the 25'h percentile CV. SA.2s = 0.21, as determined by the USEPA for the method and endpoint. CV = Coefficient of variation of the IC25 values. Control and warning limits were established using the standarddeviation of the IC2 values corresponding to the 10%nd25apercentleCVs These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint. USEPA Recommended Control and Wamnne Limits' Warning LAmit - Standard deviation corresponding to the 7 5' percentile CV. SA 7, = 0 38 ControlLinit= Standard deviation corresponding to the 90' percentile CV SA90-045. USEPA. 2000 Understanding and Accounting for Method Vanability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Ctncinnati, Oil. Organisms obtainedfrom Aquatic BaSystems,. Inc 0.81 0.77 0.79 0.80 0.80 0.81 0.81 0.83 0.84 0.84 0.85 0.84 0.84 0.84 0.83 0.84 0.83 0.83 0.82 Cv 0.07 0.13 0.11 0.11 0.10 0.12 0.11 0.13 0.12 0.12 0.12 0.12 0.12 0.11 0.11 0.10 0.10 0.12 0.12 !-08-02.xit Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pirnephales promelas using Moderately Hard Synthetic Water 1.2 1.1 1.0 0.9 0.8 0.7 06 0.5 0.4 0.3

.I I

I I I I I 0 0-Test date 7-day IC 2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. F- -Central Tendency (mean IC22) ....... Control Limits (mean IC25 +/- 2 Standard Deviations) Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 0 0 0 0 0 Test date 1.2 1.1 1.0 Qn 0.9 0.8 0.7 0.6 0.5 0.4 0.3 7-day IC 25= 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population. Central Tendency (mean IC25) Warning Limits (mean IC25 +/- SA 10J SA.1o = 0.12, as determined by USEPA for the method and endpoint) S........ Control Limits (imean IC25+/- SA 25' SA 25 = 0.21, as determined by USEPA for the method and endpoint) Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Tes nube Tet dte Control Control Mean Test mber Test Growth (%.) (mglra) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 I5 16 17 18 19 20 12-12-01 01-04-02 02-05-02 02-13-02 02-26-02 03-28-02 04-23-02 05-07-02 05-23-02 05-24-02 06-04-02 06-11-02 08-06-02 08-13-02 08-20-02 09-10-02 09-17-02 10-01-02 10-01-02 10-08-02 95 95 97.5 100 100 100 100 97.5 too 97.5 100 100 97.5 100 100 100 100 97.5 100 97.5 0.824 0.799 0.948 0.648 0.642 0.629 0.712 0.458 0.378 0.444 0.682 0650 0.694 0.659 0.765 0.854 0.824 0.750 0.975 0.929 Cv cr MSD PMSD cr for Control Growt OwCV r(o) 16.6 10.1 6.9 8.0 3.3 11.2 12.8 12.3 9.9 21.3 14.0 3.7 22.2 13.6 8.5 1.2 13.4 18A 12.7 80 13.3 11.2 10.4 9.0 9.3 9.8 10.1 10.1 11.2 11.5 10.8 11.7 11.8 11.6 11.0 11.1 11.5 11.6 11.4 (%) for PMSD (%) 0.19 0.18 0.21 0.10 0.11 0.13 0.19 0.07 0.09 0.11 0.15 0.11 0.16 0.12 0.12 0.11 0.12 0.19 0.13 018 23.5 22.0 22.2 15.5 16.5 21.4 26.2 15.4 22.5 25.0 22.1 16.6 23.5 17.5 16.3 12.5 14.6 25.4 12.8 19.5 22.8 22.6 20.8 20.0 20.2 21.1 20.4 20.6 21.0 21.1 20.8 21.0 20.7 204 19.9 196 19.9 19.6 19.6 Note-Average control growth (mg/larvae) = 0.713 CV =Coefficient of varation for control survival. On average, the CV for control growth is 11.4% in Environmental Testing Solutions. LLC Pimephales chrome toxicity tests. Lower CV bound determined by USEPA (0I percentile) 3 5% Upper CV bound determined by USEPA (90P percentile) = 20% MSD - Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the mminimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.6% from the control Lower PMSD bound determined by USEPA (10a percentile) = 9.4%,. Upper PMSD bound determined by USEPA (90& percentile) = 350/. CT = Central Tendancy (mean Control Growth CV or mean PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Punephales growth in chronic reference toxicant tests USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003 US Environmental Protection Agency, Cincinnati, OH. Organisms obtained from Aquatic BioSystems. Inc. 10-08-2.Xls Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pinephales promelas using Moderately Hard Synthetic Water Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. S- -Central Tendency (mean PMSD) Lower and Upper PMSD Bounds Lower PMSD Pound (10th percentile) = 9 4%, Upper PMSD Pound (90th percentile) - 35% (Lower and upper PMSD bounds were determined by USEPA for the method and endpoint) 40 30 20 10 0 ."nvironmental Testine Solutions. LLU Dilution preparation information: Comments: KaCHM number-4M 00o 7 Stock preparation: 50 g KCIL: Dissore 50 g KCI in I-L Deionizad waiw Dfution m (m*..) 30 450 600 750 900 Stock volume (mL) 6 9 12 15 18 Diluet volume ( L) 994 1991 988 985 982 Test organism information: J q S Test informato n: Organism age: 4Z4q I-ktJot OLD) 1Randonmiing template: ft:0 Date and times organisms &o -M iSO KM%- Incubator ntmber: were born between: 12o 4-6 JSo ___T 2 Organism source: A* £RTCfl Ir-0"1-a7. Artemia lot nurber.

sOaC Transfer bowl information:

pH = Temperature = Oven temperature: 3 o 2.. "I"I__ 7.9 rj 2q. Dry9_14i0. Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 1(0910 l~' ISCO I c 10c ot02.- /02 17 ,146 W A O 2 10"-10-o.-. 055* 160:0 1509 3 Il-Ol O0, 16ion I1l* 4 10_17--o__ 11W 1'100 s l.,*o'7. (f*'t ,*o01*2*, A/ 6 I,k a -L. 1 0 Q -. /. Control information: c rptamaia Summary of test endpoints: % Mortality: =2. S, 520% 7-day LC50 __0____P Average weight per larvae: 1, 92 Ž > 0.25 mgarne NOEC /so LOEC ChV 519.10 IC25 5 5zs.1 Potassium Chloride Chronic Reference Toxicant Test (F4PA/600/4-91/002 Method 1000.0) Species: Pimephalespromelas PpKCICR Test Number _Pp~CcR Test Number. 3k Day 0 2 3 4 5 6 7 A - Pan Wei&h ( g) B - Pan + Larvae Wei&h (g Larvae weight Cm!A-B CONTROL A B C B 0c 10 () 01 10 10 10 J1 4) /0 Suvvland Growth Data, 300 m TW~lL E F -G IH /0 / t C A) 10 10 1c) fU c) 10 / 450ma KCIIL I J K L 10 10 ic 10 /c(01c 1c0 10 /0 /0 /0 10 to to P c) 1,0 akculations and data reviewed. Comments: es Nub or.,) PpKCICR Teat Numb=r Calculations and data reviewecl. Comments: Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR # 36 Test dates: October 8-IS, 2002 C. tratio. (./. L stip t 11w1 n ..inben Of Flarn.br rot A -P...dghi (in B.-

Larv Laar.6& (a"g W.I-.5-.1 m.i r e.g.

I Mr,. MInJwd.01 )0,1111 H KC1) lar.a Imta xde (mig) - A-Bl (.) (%o him" (%) eird I%) A 10 9 14867 24230 9363 09363 Control 10 10 15012 25050 10 038 10038 97S 0.9211111 Notappiable C 10 10 14964 23220 8 256 0 8256 D 10 10 15086 24580 9494 09494 E t0 10 14933 23960 9027 09027 300 F 10 10 14984 23750 8766 08766 1000 0824 29 llu G 1 0 10 15 16 1 2 0 4 3 0 5 2 6 9 0 5 2 6 91 0 . 0,42 .1 3 II10 10 15 005 24 040 9035 0903S I 0 I0 14 807 23 100 8 293 0 8293 450 10 10 15257 23 170 7.913 07913

97.

0.22" 53 19.7 K 1 10 10 14 689 23 6 2 8 931 0 8931 9 *0 S 5$ SI L I09 14 777 22 820 1 8 043 1 0 8043 1 0 1 7 14 739 20 700 1 5 961 1 0 5961 600 N 10 6 15 131 21300 6169 06169 700 03793 37A O10 1 7 14 99 1 1 20 370 5 379 1 0 5379 7 .7 36 83 . P10 1 8 14646 20310 5664 1 05664 Q

10 1

2 15 039 17 070 2 031 0 2031I 750 R 10 1 4 14 797 18 320 3 523 0 35i23 S 10 3 15 185 17130 1945 01945 250 0.2178 44.5 7" 900T 10 2 15 149 16360 1211 01211 U 10 3 14 751 17 280 2 529 0 2529 900 V 10 2 15 160 1 16840 1 680 0 1680 IO013

0.

7 W1 0 1 1 5 0 5 4 1 1 5 3 8 0 0 3 2 6 0 0 0 3 2 61 5 0. 1 31 4. 7. X 10 0 0000 1 0000 0000 000001111 Dunnett's MISD value: PMSI). 01814 195 MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control md btmicamt datim =be dolired statistically significant in a whole effluent toxicity test On average, a significant difuence occurs fr avfnorm Wal Teasta 8 Solutions, LLC chronic toxicity tests when a toxicant reduces Punephales growth by 19 6% fior the control (deteramned through reference toxicant testing) Lower PMSD bound determined by USEPA (10th percentile) - 9 4%. Upper PMSD bound determined by USEPA (90th peroentile) - 35%. The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 labror ierws for Pimephda growth in chronic reference toxicant tests. USEPA 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program EPA-833-R-00-003. US Envhimn" Pt. Agency, Cincinnati, OIl Organisms obtained from Aquatic BwSse em, lic 10-08-02jds Pevelived by- hfil 7 Day Growth ~R D4Cotrol0 0 0-I 450. P u t t

750,

~Response0 00 0jý ý M 900 .a... z0i pp3i .J I.p 0 i0 ~~ ~ P 0 oI E.,' PpKCICR Test Number-t (o MWS Control Chemistry: Acceptance Criteria for Daily, Chemistry: Acceptance Criteria (dilutions must be remade if the conductivity falls outside the ac Itble ragn) 280-360 840-890 1100 -1180 139 - 146 1660- 1710 1890 -1950 67000 - 76000 Concentration CONTROL 300 mg KCI/ 450 mgKI/ 600 mg KC1/L 750 mg KCI/L 900 mg KC1/L KCI Stock (25 -g KCI/L) lot PpK~CICRTest Number. 34' Day Le(,050 yqooO 4g~ &9jol D 1015 PpKCICR Test Numbe. a ( 1 r = m I 4 Mcitraflon I Parameter MU I I 6 PH (S.U.) sp -4.64 A -."bl 6 q.96 F'ý.99 DO (mg/L) Iq I -i-el Conductivity (Mpih&cm) -3 112-33 0 1 Temperature 24.6 24.4 -742 (OC) pH (S.U) B.Co -DO (mg/L) L iG5 -4 n Conductivity (pinhWan) 86zp ]o 94q Temperature (OC) -24..7-lz,5. 9Z zq.1 2q,-l --z4. ZS.,3 PH tS.U.) e00 4 R

a.

8t( DO (mg/L) -o .(, L Az 1 - 4 (prnhos/cm) Iio 1qla Temperature 4 0 21 L. e, z7 z-7 L.. 2s nmg KClJL -DO (mngL) S 000 Conductivity Temperature zq.A ?-0~ Zq. z z'/ 7 2~ ~ ~~ PH (S.U.) 80 A 5.1 7Sc DO (mg/L) 4.o .0 -o 1mg KCIIL -Conductivity 4 (gzmhos/cm) (4s05ZL Temperature COC) Z4.6 --fE V2 25.2-~ ZV1 -2q.-l 2q. iQ DO (mg/L)

8.

30 6.0 f3X. 3 mg KCUL Conductivity 0 Temperature P

qz~

~.4 7. I JALIU4I II UIaI I IflIYIflI TI KITtI I Ini4iel II I II ,* I II -I ~ TI k iua I lidniai 1 final 1 ONTROL I mg KCI/L I mg KCI/L Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test number Test date 7-d IC 2 5 CT (gIL NaCI) (g/L NaCI) S Control Limits CT-2S CT+2S SIo Warning Llmits CT - Sl, CT + SA.1, SAJs Control Lmilts CT-SAm CT + SA, 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Is 16 17 18 19 20 03-07-01 04-04-01 05-09-01 06-06-01 07-10-01 08-08-01 09-12-01 10-10-01 11-01-01 12-05-01 01-09-02 02-05-02 03-19-02 04-09-02 05-07-02 06-04-02 07-09-02 08-06-02 09-04-02 10-08-02 I 04 1.02 1.10 1 07 1 05 I 02 1 02 I 03 I 00 I 05 0.91 1 07 1 03 1.03 I 05 1 06 I 03 1 05 1 05 I 03 1 03 1 05 1 06 1 06 1 05 1 05 1 05 1 04 1 04 1 03 1 03 1 03 1.03 1 03 1 04 1.04 1 04 1 04 1 04 001 004 003 003 003 003 003 003 003 005 005 005 004 004 004 004 004 004 004 101 097 099 1 00 0.99 098 099 098 098 0.93 094 094 0 94 095 0 95 0 95 0.96 0 96 096 1'.06 1 14 1 13 1.12 1.11 III 110 1 10 1.10 1.13 1.13 1 12 1.12 1.12 1 12 1.12 1.12 1.11 1.11 008 008 008 008 008 008 008 008 008 0.08 008 008 008 008 008 008 008 008 008 0.95 097 0 98 098 0.97 097 097 096 096 095 095 095 095 095 096 096 096 096 0.96 Note-7-d IC 25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. CT = Central tendency (mean IC2s) S = Standard deviation of the IC2s values SA.j0 = Standard deviation corresponding to the 1 06 percentile CV SA 1s = 0 08, as determined by USEPA for the method and endpoint SA.2s = Standard deviation corresponding to the 25' percentile CV. SA.*5 = 0 17, as determined by the USEPA for the method and endpoint. CV = Coefficient of vanation of the IC25 values Control and warning linuts were established using die standard deviation of the lC25 values Corresponding to the 0aand25spercentileCVs Theseragesarenmoreasringenthan1 thecontrol aix waning limits recommended by US EPA for the test method and endpoint UISt.PA Recommended Control and Waminli I1miuts Warning I init = Standard deviation corresponding to the 7 5d percentile CV SA 7s = 0 45 ControlLimit-Standard deviation corresponding to the 90t percentileCV SA *- 062 IJSLPA 2000 Understanding and Accounting for Method Vanability in Whole Effluent Toxicity Applications Under the Natinad Pollutant Discharge Elimination Program. EPA-833-R.-00-003. US Fvhomita Protection Agency, Cincinnati. OIl 1.11 1 13 1.14 1.14 1.13 1.13 1.13 1.12 1.12 1.11 1.11 1.11 1.11 1.11 1.12 1.12 1.12 1.12 1.12 0.18 018 0.18 0.18 0.18 0.18 0.18 018 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.18 0.86 0.88 089 0.89 0.88 088 088 087 0.87 086 0.86 086 0.86 0.86 0.87 087 087 087 0 87 1.20 1.22 1.23 1.23 1.22 1.22 1.22 1.21 1.21 1.20 1.20 1.20 1.20 1.20 1.21 1.21 1.21 1.21 1.21 0.01 0.04 003 0.03 003 003 003 003 003 005 005 004 004 004 004 004 004 004 00o4 IO-O9O-.xh CV Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water O O a-II I Test date 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for tie test population. Central Tendency (mean IC 2 ) Control Limits (mean IC25 +/- 2 Standard Deviations) 1.5 1.4 1.3 p 1.2 1.0 0.9 0.8 Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 4 OK /0k01 05 ok OR 414 4ý 0ý 0ý 02 4O, e0, Ot Ot0. 4 O Test date 1.5 1.4 1 1.3 U S1.2 1.11 1.0 0.9 1 0.8 I I I I I I I I I I I I I i I I I I Warning and Control Limits Set According to 10O' and 25'h Percentile C Vs. S................................. .. o..... o....* I I I I I I I I I I I t I I I I I I 7-day IC 25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean ICz2 ) Warning Limits (mean IC25 =+/- SA 10, SA 10= 0.08, as determined by USEPA for the method and endpoint) ......... Control Limits (mean IC25 + SA 25' SA 25 = 0.17, as determined by USEPA for the method and endpoint) I I I I I A I I I I I S...

- - 6 O*.................

Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test Test date Control Control Mean number Survival Reproduction (%/) (,of~pingl~ficmae) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 03-07-01 04-04-01 05-09-01 06-06-01 07-10-01 08-08-01 09-12-01 10-10-01 11-01-01 12-05-01 01-09-02 02-05-02 03-19-02 04-09-02 05-07-02 06-04-02 07-09-02 08-06-02 09-04-02 10-08-02 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 24.0 25.0 27.9 27.8 25.0 30.9 28.6 24.9 27.0 24.8 27.5 23.1 26.3 26.2 27.3 26.0 29.5 28.4 31.4 31.1 Cv cr for Control els) Reproduction CV (%/) 8.3 10.0 8.9 5.8 9.4 2.4 6.6 8.8 6.5 9.1 7.5 10.1 8.6 12.3 8.1 9.9 8.0 10.4 6.7 9.2 9.2 9.1 8.2 8.5 7.5 7.3 7.5 7.4 7.6 7.6 7.8 7.8 8.2 8.2 8.3 8.3 8.4 8.3 8.3 MSD PMSD CT (e/) for PMSD (%4) 2.4 2.9 3.1 3.5 2.8 2.7 2.7 2.5 3.1 4.8 4.5 2.6 3.0 2.8 2.3 3.8 3.5 2.7 3.0 2.9 10.1 11.8 11.0 12.5 11.2 8.7 9.4 9.9 11.6 19.5 16.3 11.1 11.2 10.7 8.4 14.7 11.7 95 9.5 9.4 10.9 11.0 11.3 11.3 10.9 10.7 10.6 10.7 11.6 12.0 11.9 11.9 11.8 11.6 11.8 11.8 11.6 11.5 11.4 Note Average control reproduction (offspring per female) = 27.1 CV = Coefficient of variation for control reproduction. On average, the CV for control reproduction is 8.3% in Environmental Testing Solutions, LLC Lower CV bound determined by USEPA (104' percentile) 8 9% Upper CV bound determined by USEPA (9 0' percentile) = 42% MSD = Minimum Significant Difference PMSD = Percent Mmunum Significant Difference PMSD is a measure of test precision. The PMSD is the minnunm percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant differente occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphria reproduction by 11.4% from the control Lower PMSD bound determined by USEPA (I0& percentile) = 11% Upper PMSD bound determined by USEPA (90e percentile) 37% CT = Central Tendancy (mean Control Reproduction CV or mean PMSD) The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cenodaphnia reproduction in chronic reference toxicant tests USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH. 10-08-02.xls Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 50 45 40 35 S30 S25 20 15 10 5 0 Test date , <p

0 0 0) 0735521972 1 035 0 130465747 0 157285946 Bardees Test ndicates equal varinces (p - 0.30) 6050591469 15 08631706 Hypothesis Test (1-Call 0 0) NOEC LOEC CEV TV MSDU MSDp ESB MSE F-Prob df Duaneus Test goo 1000 894427191 2923066691 0093989283 1759 346667 617037037 2.38-34 5,54 Lin*ar Interpolation (80 Resamples) Point mgIL SD 95% CL Skew IC05 818.2051232 89 1717353 533 6076555 373 3362691 -1 4549 [CID 8979487179 5419916136 767.2638889 9617269737 .08484 [CIs 9776923077 39.31064085 8757670773 1009 83257 -09661

10.

-C20 1011.256281 1576437826 9537145333 1024769496 -2.2154 1025 1026834422 9011151569 1006663923 t039706422 .0.2877 IC40 1073768844 768929744 1058848531 1088.553333 -00946 08 ICso 1105025126 77S7889513 1091412451 1122229167 00342 07 060. 805 004 03 0.2 01 0 0 1 0 500 1000 1500 Dose mgtL Dose-Response Plot 35 30 o25 -2 0 o15 1-tail, 0.05 level of significance lO-08-02.xls CdNaCICR Test Number ?:. MHS Control Chemistry: Parameter Control Batch: 16.o:/.07_1 fo-CA-61 lo4(rz-_ pH (s.u.) "8 .7 7* DO Conductivity (=os/cmn (n CaCO3 L) ( v-2-_ 60o Hardness (mg CaCO3/L) Acceptance Criteria for Daily Chemistry: II Acceptance Criteria Concentration (dilut~ons mu be remnade ifthe cnutvt fall outside the acce~tale range) CONTRiOL 280 -360 600 mg NaCI/L 1390- 1590 800 mg NaCIIL 1780 - 1970 1000 mg NaCIIL 2190 - 2370 1200 mg NaCIIL 2550 - 2740 1400 mg NaCIIL 2910-3160 NaCI Stock 110500 - 116300 Lj &AUAA-31 JLdJULI Environmental Testing Solutions, LLC Page 6 of 7 CdNa]CR Test Number. looq oo 1osi /Illoo Invi ronmientail estin~ Solutions. LL UaeI0 CdNaCICR Test Number.2-Concentration Parametr345 pH (S.U.)

q. A-5 I-of B9 3.n 1 84 CONTROL Conductivity pH (S.U.)

Q.OL0 80.0 .02 6.DS 9 (.0,Z DO6g/) 1.18. SOO mg NaCI/L Conductivity (iimhos/cm) 15!104 Temperature 7 L1C q7 A9 2-., 8.c0 ...7q 7- -' 7` pH (S.U.) 8 C)z00 S00 B1 scoDO (mgi'L) 8.1 9 6.0.6. 1000 ng NaClfL Conductivity

25.

2o Temperature '2A.6 L 2-- q.vf7 Z'J. L s~ DO (mg/L) 8.1 83.0 606. 1200 mg NaCIIL Conductivity 263 2O0Z 1200 mg NaC1IL Conductivity 2g69U0 Temperature v-zus 2S.I zq.z.? 17Z. Tepeaur Z.

z.

.2,/.-7 z'/.¶ 7G ZS-O nialIFInitial nta IFal1Initial i Final iInitial I[ FinalI 108'[000a 1016)o lLfY rdgc 1 01 1 16 (a 7w

ML030410121

Text

Navigation menu

Search