ML022130393
| ML022130393 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/11/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML022130393 (122) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 July 11, 2002 State of Tennessee Department of Environment and Conservation
.Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Please find enclosed the Discharge Monitoring Report (DMR) of June 2002 for Sequoyah. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pnnted Ow -ecyled -oer
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2A)
_ ___$_S.LY - DAISY
_TM 3738B Facili A -
_R_
Location HAMILJON COUN ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 101 G F - FINAL PERMIT NUMBER DISCHARGE NU)MBR DIFFUSER DISCHARGE lA(3tNIT/TPDINr(_' PPI(Dlrf Form Approved.
OMB No. 2040-0004 EFFLUENT F FAro Mm DAY VFAr Me DAY NO DISCHARGE NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING I
QUALITY OR CONCENTRATION NO.
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EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG.
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CL NAME/fITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my t__
/
TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or 1U LA-persons who manage the system, or those persons directly responsible for gathering the Environmenfal Supervisor Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, EnvironmentalSupervisor 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
TYEDORPRNTDOFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I___________________________I___
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.053 mg/L--limit 0.100mg/L) 2. CL-363 (max. calc. conc. was 0.012 mg/L--limit 0.100mg/L) 3. PCL 222/PCL-401 (max. calc. conc. was 0.0188mg/L--Iimit 0.100mg/L) 4. H-130M (max. calc. conc. was 0.022mg/L--limit 0.050mg/L)
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page 1 of 2
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000
-f.
IfNTFROQFFICE SB-2A SD.y-AISY TN 37334 Facility TVA - SEQUOYAH NUCLfARP2LN_
Location HMILTQL.
O NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
T 26450 1
101 G PERMIT NUMBER IDISCHARGE NUMBER MAJOR (SUBR 01)
F - FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved.
OMB No. 2040-0004 ATITN: Diedre B. Nida I YEAR MQ I DAY.I I
YFAR IMhdQ I DAYI From l 02 06 _01 I
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QUALITY OR CONCENTRATION NO.
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EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE
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0 0
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'/
TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I
TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alf attachments here)
CCW data for June 2002 is attached.
itr-A rorllmOZ' M20-rj:V 3,l9) t",feVibULed.
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Page 2 of 2
CCW Data for June 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
June 4, 2002
< 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
June 4, 2002 0.6 Page 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2A.
5_D_.
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7
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DISCHARGE MONITORING REPORT (DMR)
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F - FINAL DIFFUSER DISCHARGE EFFLUENT Form Approved.
OMB No. 2040-0004
.*. NO DISCHARGE F
NOTE: Read instructions before completing this form.
UNITS IC25 STATRE 7DAY CHR CERIODAPHNIA TRP3B 1
0 0
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0 0
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>100 I**
(23)
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EX FREQUENCY OF ANALYSIS
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SAMPLE MEASUREMENT NAME/FITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my
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ii'f TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor 02 07 11 information, the information subrttted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY T Y P E D O R P R IN T E DO F I E O R A T O I E A E NA R AN M EY A R MD Y
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled from 5/5/02 to 5/10/02. The report is attached.
I I
EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2A.
DDY - DAISYT T_
M37a84 Focilitv
.I..
Location HAMJLTON COIy--_
ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TNo02640 I03 PERMIT NUMBER DISCHARGE NUMBER I
MC)NIT(')FIN(- PF~IC)n YFAPl MO' DA rm l
ý Yr Mn I UAYI From 0 2 0 6 01I Tol 02 Io MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL LOW VOL. WASTE TREATMENT POND EFFLUENT
.*. NO DISCHARGE E:]
NOTE: Read instructions before completingt this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE
- 7.2 8.7********
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REPORT...
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NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Il Cetf under penalty of law that this document andc all attachments were prepared under my
.IL)TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Environmental Supervisor Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
I TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DArY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3/99)
Previous editions may 1be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE S8-2_AA
- __SODQ~Y - DAISYL
-_ TN a37384 Facility TVA ---EQC-Y-HN_.CLEAR.LL Location HAAMILTQ_1_C____U.
ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TN06450O17 PERMIT NUMBER DISCH 1 RGE NUMBER MONITQr?IN(-: PERIOD YEAR L.LM DAY I I YEAR* I O
DA From 02 106 o1 1 To 102 1 06 j30 MAJOR (SUBR 01)
F - FINAL METAL CLEANING WASTE POND EFFLUENT NO DISCHARGE IF NOTE: Read instructions before con I
Form Approved.
OMB No. 2040-0004 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Richard T. Purcell Site Vice President TYPED OR PRINTED
-Irir-etion nr Stinlrri-inn ij linciuaing tme law that this document and all attachments were prepared under my n accordance with a system designed to assure that qualified personnel uate the information submitted. Based on my inquiry of the person or system, or those persons directly responsible for gathering the on submitted is, to the best of my knowledge and belief, true, accurate, a that there are significant penalties for submitting false information, I fine and Imprisonment for knowing violations.
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No phosphate bearing chemicals were employed. No Discharge this Period rrovtuua uuirtorts may ae useo Page 1 of 1 EPAf r-orm 3320,-U-1 kR-V 199I)IU P~revious eumIuOIs may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2A.
OQDY-_DAILSY TN 37M384 Facility TA - EQUQY Ht_ NUCLEAR PLANT Locotion__-AMILTON COgUN]y NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 I 1 110 G F - FINAL PERMIT NUMBER IDISCHARGE NUMBE RECYCLED COOLING WATER Mh-NITflDIN(FJ PfZDlnrt Form Approved.
OMB No. 2040-0004 EFFLUENT ATTN: Diedre B, Nida Frm I YER 40To I8A1O From 1 02 0 6- 0-11 Tol 1o2 1 06 -130
... NO DISCHARGE
.XX..
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT (12) 00400 1
0 0
PERMIT
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6.0 9.0 SUDAILY GRAB EFFLUENT GROSS VALUE 9UEMN
'f.
,MIMUMAI M________
SOLIDS, TOTAL SUSPENDED SAMPLE
-- MAXIMUM MEASUREMENT (19) 00530 1
0 0
PERMIT 3/4Ž~**~
MGL AIYC PO EFFLUENT GROSS VALUE
- QUIREMENT AL:_______:
OIL AND GREASE SAMPLE MEASUREMENT (19) 00556 1
0 0
4PERMIT 4*~~.MIL~~~ALY qMO EFFLUENT GROSS VALUE REQUIREMENT-15,,.
MGAILAILYX'MP COPPER, TOTAL (AS CU)
SAMPLE MEASUREMENT (19) 01042 1
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EFFLUENT GROSS VALUE DAILY X
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EFFLUENT GROSS VALUE REURMN
- 1.
DAIL COMPOS PHOSPHORUS, TOTAL (AS P)
SAMPLE (19)
MEASUREMENT (19) 00665 1
0 0
'PERI9MT REQUIREMENT'
- 1.
MGIL DAILY COMPOS EFFLUENT GROSS VALUE DAILY M FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT (03) 50050 1
0 0
MGD R
T R)'.*4EPORT CAY ALCTD EFFLUENT GROSS VALUE REQUIREMENT.
MO AVG~
DALY NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ~I Certify under penalty of law that this document and all attachments were prepared under my (I
.4,/)TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or C.y 1,
persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor information, the information submitted is. to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AR YEAR MO DAY T Y P E D O R P R IN T E D O F C R O U H R Z D A E TA E I N M E E R M
A COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period I-re vious editions may be usea I
EPA* Form 3,320-1 (RilEV,./19)
Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2A.
S..
Y
__S D
&_Y=D ISY_. -_ TN 37"384 Facility_
T\\VA -EQUOYAH NU.QL.ERPL*N**
Location HAMILTCQOU.N NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR)
TN 6450 1
PERMIT NUMBER IIDISCHARGE NUIMER:
Form Approved.
OMB No. 2040-0004 MAJOR (SUBR 01)
F - FINAL BACKWASH EFFLUENT ATTN: Dledre B. Nida From o
o Qo Y
I Yo A
M2I I DAY NO DISCHARGE L NOTE: Read instructions before comolefino this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE
- 0 (00*0
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0 0
PERMIT r
R P T
PAS0EE VISUAL EFFLUENT GROSS VALUE R
UIE NT FAIL=1 PFMT___
SAMPLE MOTTLPRI MEASUREMENT PERMIT REASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT MEASUREMENT REQUIEMENT SAMPLE MEASUREMENT REQUIREMENT
<4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cenify under penalty of law that this document ard all attachments were prepared under my 1 - 1 l
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell property gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor Site Vice President information, the information submitted is. to the best of my knowledge and belief, true accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ottachments here)
Operations is required once per shift to perform visual inspections.
P'revious editions may be used E:PA/ Form 3320-1"U° (RE::V 3,'/99)J Page I of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Nome TVA - SEQUOYAH NUCLEAR PLANT Address BOX 200 (INTEROFFICE SB-2A.
_ __. _QDD__-_DALSL -_ M 37384.......
Facility TVA - SEQUOYA NUCLFRLANT Location J-H AM TITN _.CO U NT--
NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR)
(SUBR 01)
TN0026450 G
F - FINAL PERMIT NUMBER DISCHARGE NUMBER BACKWASH
._M_)NITlNP_
.___lf_
EFFLUENT ATTN: Diedre B. Nida I YEAR MC D-AY I
YIARI MC ID)AyI From 1 02 I06 01 To 1 02 06 -- 370
- NO DISCHARGE L[_i NOTE: Read instructions before comoletino this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY)
SAMPLE 0
(00) 1 /*30 VISUAL MEASUREMENT (00) 01345 1
0 0
PERMIT REPORT.
PASS=0 SEE VISUAL EFFLUENT GROSS VALUE REQUIREMENT.
FAIL=1 PERMIT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT R EQUIREMENT k
SAMPLE MEASUREMENT (PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my fl
/
t TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel CQA4 Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or
.Y1 I
Sersons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE i
including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREA I NUMBER YEAR MO DAY TYPED OR PRINTED I
RAMR M
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ottochments hefe)
Operations is required once per shift to perform visual inspections.
P-revious editions may be useo Form Approved, OMB No. 2040-0004 EPA Form 3320-1U- (R'ElV 3/99)
Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)
Name TVA - SEQUOYAH NUCLEAR PLANT Address BOX 2000 (INTEROFFICE SB-2Aý -
SO5DDY -__DA_.Y- - TN 37384...
Facility TVA - SEQUOYAH NUCLEAR2.NT Location HAMI LTQONOTY ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) 0MB No. 2040-0004 TN0026450 1
118 G
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FF-FINAL PERMIT NUMBER IIDISCHARGE NUM WASTEWATER & STORM WATER hAnr-jITnDIfI\\l*r-PPDIrt'-
F Vro Ar MOL I"DATYO YA" I
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AY From 1 02 0 6 1 01 1 To102 1061301 EFFLUENT NO DISCHARGE 5 NOTE: Read instructions befnre, Onmno.tino thi.* fnrm PARAMETER QUANTITY OR LOADING 1
QUALITY OR CONCENTRATION NO.
FREQUENCY SAMPLE I
EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO)
SAMPLE MEASUREMENT (19) 00300 1
0 0
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GR9AB EFFLUENT GROSS VALUE REQUIREMENT-
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SAMPLE MEASUREMENT PE~RMIT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared underm f/
/
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
O TYPED OR PRINTED OFCR.RATRIED AGENT AREA INUMBER YEAR MO DA COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oil attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period I
=
I EPA Form 3320-1 (REV 3/99)
Previous editions may be used Page I of 1
June 26, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2002 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report is included for your records. The report provides results of compliance testing using fathead minnows and daphnids.
Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent. Daphnid reproduction was significantly different from control based on results from the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN May 2002m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE
SUMMARY
- 1) Facility/Discharger: Sequoyah Nuclear Plant/TVA Report Date: June 26, 2002
- 2)
County/State: Hamilton / Tennessee
- 3) NPDES Permit #: TN0026450
- 4)
Type of Facility: Nuclear-fueled electric generating plant
- 5)
Design Flow (MGD): 3
- 6)
Receiving Stream: Tennessee River (TRM 483.6)
- 7) 1Q10:2,992.4
- 8)
Outfall Tested: 10_1
- 9) Dates Sampled: May 5-10, 2002
- 10) Average flow on day(s) sampled (MGD): 851, 869, 875
- 11) Pertinent site conditions: No unusual conditions reported. *
- 12) Test Dates: May 7-14, 2002
- 13) Test Type: Short-term Chronic-definitive
- 14) Test Species: Fathead Minnows (Pimephales promelas)
Daphnids (Ceriodaphnia dubia)
- 15) Concentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16) Permit Limit Endpoint (%): Outfall 101:
IC5 = 43.9
- 17) Test Results (%): Outfall 101: Pimephales promelas: IQC 2 5 > 100 Ceriodaphnia dubia: IC25_> 100 UV treated Outfall 101: Pimephalespromelas: IC 5 > 100
- 18) Facility
Contact:
Diedre B. Nida
- 19) Phone #: (423) 843-6700
- 20) Consultant/Testing Lab Name: Environmental Testing Solutions, LLC 2 1 )
Lab
Contact:
Jim Sumner
- 22) Phone #: (828) 862-8193 TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- Production/operation data will be provided upon request.
- 22) Notes: Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent.
Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent.
Daphnid reproduction was significantly different from control based on result of the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms. These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
METHODS
SUMMARY
Samples
- 1) Sampling Point: Outfall 101, Intake
- 2) Sample Type: Composite
- 3)
Sample Information:
Sample Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp.
TRC*
Used (MM/DD/YY Time)
(MM/DD/YY Time)
(°C)
(mg/L)
(MM/DD/YY Time) 101 05/05/02 1450 to 05/07/02 1155 0.3 '
<0.10 05/07/02 1458 1105/06/02 1350 05/08/02 1450 05/05/02 1509 to
{05/07/02 1458 Intake 05/06/02 1409 05/07/02 1155 0.3
<0.10 05/08/02 1450 05/07/02 1224t09____
05/09/02 1446 101 05/07/02 1225 to 05/09/02 1112 0.3'
<0.10 05/09/02 1446 0105/08/02 1125 05/10/02 1440 Intake 05/07/02 1241 to 05/09/02 1112 0.3
<0.10 05/09/02 1446 05/08/02 1141 05/09/02_11 1 1
05/10/02 1440 05/11/02 1511 101 05/09/02 1318 to 05/11/02 1145 1.8'
<0.10 05/12/02 1518 05/10/02 1218 05/13/02 1514 05/11/02 1511 Intake 05/09/02 1333 to 05/11/02 1145 1.8
<0.10 05/12/02 1518 05/10/02 1233 05/13/02 1514
- Total residual chlorine.
t Collected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
2
- 4) Sample manipulation:
Non-treated samples (compliance test) from Outfall 101 and Intake were warmed to test temperature (25.0 +1.00C) in a warm water bath.
Aliquots of Outfall 101 and intake samples used for the side by side UV treated fathead minnow test were warmed to 24 0C in a warm water bath and held in a 40 Watt Rainbow Lifeguard UV Sterilizer for 2 minutes, prior to exposure of test organisms.
Test Organisms
- 1) Source:
- 2) Age:
Pimephales promelas Aquatic BioSystems, Inc.
23.0-26.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> Ceriodaphnia dubia In-house culture
< 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Test Method Summary (See Appendix A for additional information)
- 1) Test Conditions
- 2) Test Duration
- 3) Dilution/Control Water Pimephales promelas Static, renewal 7 days Moderately Hard Synthetic Water Ceriodaphnia dubia Static, renewal Until at least 60% of control females have 3 broods Moderately Hard Synthetic Water
- 4) Number Replicates
- 5) Animals per Replicate
- 6) Test Initiation: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 7) Test Termination: (Date/Time)
Outfall 101 UV Treated Outfall 101
- 8) Test Temperature: Outfall 101:
Test Temperature: UV-Treated 05/07/02-1458 EDT 05/07/02-1600 EDT 05/14/02-1409 EDT 05/14/02-1436 EDT Mean =24.5 'C (24.2-25.1 0C) 05/07/02-1249 EDT 05/14/02-1300 EDT Mean = 24.7'C (24.2-25.40 C)
Outfall 101:
Mean = 24.5°C (24.2-25.3°C) 3 4
10 10 I
- 9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)
- 1) Results of a Pimephales promelas (Genus)
(Species)
Conducted 05/07/02 05/14/02 (mm/dd/yy) (mm/dd/yy)
Chronic/7-d Toxicity Test (Type/Duration)
Using Effluent From Outfall 101.
(number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4*
5 6
7 Control 100 100 100 100 100 100 98 10.98% Effluent 100 98 95 95 95 95 95 22% Effluent 100 95 95 95 95 95 95 43.9% Effluent 100 100 100 100 100 98 98 72% Effluent 100 100 100 100 100 98 98 100% Effluent 100 98 98 98 98 98 97' Intake 100 100 100 98 88 75 68
- 96-Hour LC50 Value: > 100%
Calculated TU Estimates: t < 1.0 TUa 95% Confidence Limits:
Permit Limit: 1.0 TUa Upper Limit NA Lower Limit NA IC2 5 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
3 4
j Mean Control 0.448 0.476 0.405 0.432 0.440 10.98% Effluent 0.562 0.361 0.557 0.502 0.496 22% Effluent 0.497 0.463 0.353 0.472 0.446 43.9% Effluent 0.583 0.448 0.406 0.552 0.497 72% Effluent 0.421 0.486 0.519 0.507 0.483 100% Effluent 0.546 0.624 0.492 0.536 0.549 Intake 0.478 0.389 0.125 0.463 0.364 IC 25 Value: > 100%
Calculated TU Estimates: t < 1.0 TUc 95% Confidence Limits:
Permit Limit: 1.0 TUc Upper Limit = N/A Lower Limit = N/A One fish was lost during termination. The test concentration was initiated with 39 fish instead of 40 for statistical purposes.
tTUa = 100/LC50: TUc = 100/IC25 5
TOXICITY TEST RESULTS
- 2) Results of a Ceriodaphnia (Genus) dubia (Species)
Chronic/ 3-brood (Type/Duration)
Toxicity Test Conducted 05/07/02 05/14/02 (mm/dd/vv' (mm/dd/vv)
Using Effluent From Outfall 101.
(number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4*
5 6
7 Control 100 100 100 100 100 100 100 6.25% Effluent 100 100 100 100 100 100 100 12.5% Effluent 100 100 100 100 100 100 100 25% Effluent 100 100 100 100 100 100 100 50% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100
- 96-Hour LC50 Value: > 100%
Calculated TU Estimates: t < 1.0 TUa 95% Confidence Limits Permit Limit: 1.0 TUa Upper Limit = NA Lower Limit = NA Reproduction (#young/female/7 days) Data Test Solutions Replicates 1
2 3
4 5
6 7
8 9
10 Mean Control 25 28 31 32 24 26 25 25 29 31 27.6 10.98% Effluent 26 28 28 29 29 31 27 26 32 30 28.6 22% Effluent 26 31 26 32 29 31 30 27 25 28 28.5 43.9% Effluent 25 28 26 31 29 34 28 28 30 31 29.0 72% Effluent 27 26 30 27 31 27 27 30 32 34 29.1 100% Effluent 30 31 28 30 28 29 33 35 33 32 30.9 Intake 30 22 24 21 15 14 23 18 26 27 22.0 IC 25 Value: > 100%
Calculated TU Estimates: t < 1.0 TUc 95% Confidence Limits:
Permit Limit: 1.0 TUc Upper Limit = N/A Lower Limit = N/A tTUa = 100/LC50: TUc = 100/ IC25 RFFFRFNCE TOXICANT TEST RESULTS (See Aopendixes A and D)
S.
\\.
_7 Species Date Time I Duration Toxicant Results (I Pimephales promelas 05/07/02 1538 7-days KC1 544.6 mg/L (IC2 5)
Ceriodaphnia dubia 05/07/02 1220 7-days NaC1 1049.2 mg/L (IC 25) 6
TOXICITY TEST RESULTS UV-TREATED (See Appendix B for Bench Sheets)
- 3) Results of a Pimephales promelas (Genus)
(Species)
Chronic/7-d (Type/Duration)
Toxicity Test 05/07/02 05/14/02 (mrmdddvw) (mm/dd/yy)
Using Effluent From Outfall 101, UV treated (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4*
5 6
7 Control 100 100 100 100 100 98 98 10.98% Effluent 100 100 100 100 100 95 95 22% Effluent 100 100 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 98 98 Intake 100 100 100 100 100 100 100
- 96-Hour LC50 Value: > 100%
Calculated TU Estimates: ? < 1.0 TUa 95% Confidence Limits:
Permit Limit: 1.0 TUa Upper Limit NA Lower Limit NA IC25 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
3 4
Mean Control 0.494 0.446 0.475 0.479 0.473 10.98% Effluent 0.377 0.477 0.487 0.336 0.419 22% Effluent 0.504 0.484 0.508 0.541 0.509 43.9% Effluent 0.512 0.516 0.493 0.613 0.533 72% Effluent 0.458 0.480 0.440 0.443 0.455 100% Effluent 0.474 0.480 0.420 0.520 0.473 Intake 0.477 0.436 0.447 0.496 0.464 IC 25 Value: > 100%
Calculated TU Estimates: 1 < 1.0 TUc 95% Confidence Limits:
Permit Limit: N/A Upper Limit = N/A Lower Limit = N/A TUa = 100/LC50: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D)
Species Date Time Duration Toxicant Results (IC25)
Pimephales promnelas 05/07/02 1538 7-days KC1 536.7 mg/L (IC25)
UV-Treated 7
Conducted
SUMMARY
/CONCLUSIONS Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent. Daphnid reproduction was significantly different from control based on result of the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
8
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1) Pimephales promelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2) Ceriodaphnia dubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL
- 1) Pimephales prom elas None
- 2) Ceriodaphnia dubia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1) Pimephales promelas None
- 2) Ceriodaphnia dubia None 9
PHYSICAL AND CHEMICAL METHODS
- 1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2) Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3) Temperature was measured using EPA Method 170.1.
- 5) The pH was measured EPA Method 150.1.
- 6) Conductance was measured EPA 120.1.
- 7) Alkalinity was measured using EPA 310.1.
- 8) Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1) Test Type: 7-day chronic tests with results expressed as IC 25 values in g KC1.
- 3) Dilution Water Used: Moderately hard synthetic water.
- 4) Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1) NPDES Permit No. TN0026450.
- 2) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).
- 3) Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
10
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Date Sodium Towerbrom PCL-222 PCL-401 hypochiorite mg/L TRC mg/L TRC tin/gL mg/L
__________Phosphate Copolymer 03/12/1998 0,016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0.015 0.014 0.005 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998
<0.001 0.044 0.015 09/13/1998
<0.001 0.044 0.015 09/14/1998 0.008 0.044 0.015 02/22/1999
<0.00:1 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999
<60.001 02/28/1999
<0.001 08/18/1999 0.015 0.069 0.024 08/19/1999 0.012 0.024 08/20/1999 0.023 0.070 0.024 08/21/1999 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068.
0.024 08/24/1999 0.016 0.067 0.023 Cuprostat PF mg/L Azole 0.120
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat hypochio~rite PF mg/L TRC mg/L TRC mg/L mg/L mg/L mg/L Azolt Phosphate' Copolymer DMIAD 01/31/2000
< 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 "
02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000 0.0057 0.055 0.019 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000 0.0088 0.055 0.019 07/30/2000
< 0.0076 0.055 0.019 07/31/2000 0.0152 0.055 0.019 0-006 08/01/2000 0.0141 0,055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.00OO5y" 12/13/2000
<0.0120 0.025 0.020 12/14/2000 0.0087 0,025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 0.0036 0.025 0.020 12/17/2000
<0.0036 0.025 0.0201 08/26/2001 0.017 0.06 0.021
-i 0.006 08/27/2001
<0.0096 0.06 0.021 0.005 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 0.020 0:oO5 08/30/2001
<0.0123 0,06 0.021 0.005 08/31/2001
<0.005 0.059 0.020 11/25/2001
<0.0044 11/26/2001
<0.0119 0.024 0.02 0.005, 11/27/2001 0.0137 0.023 0.019 0.007' 11/28/2001
<0.0089 0.022 0.019
, 0.006 11/29/2001 0.0132 0.024 0.02 0M007 11/30/2001 0.0043 0.024 0.02 12/09/2001
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128 0.02 0.008 12/13/2001
<0.0088 0.024, 0.02 12/14/2001 0.0134 0,024 1
0.02 0.007 j
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Date Sodium Towerbrom
, PCL-222 PCL-401 CL-363 Cuprostat hypochlorite mg/L TRC mg/L mg/L ig(L.
PF mg/L mg/L TRC Phosphate Copolymer DMAD Azole 1/02/2002
< 0.0079 0.023 0.02 0.006 1/03/2002
< 0.0042 0.023 0.014 1/04/2002 0.0124 0.024 0.014 0.009 1/05/2002 0.0042 1/06/2002
<0.0042 1/07/2002
< 0.0089 0.024 0.014 0.006 6 2/24/2002
<0.004 2/25/2002
< 0.004 0.023 0.023 2/26/2002 0.0143 0.023 0.023
{0.007 2/27/2002
<0.0041 0.023 0.023 2/28/2002 0.0041 0.024 0.008
(*
3/01/2002
<0.0041 0.024 0.008 5/05/2002
<0.0070 5/06/2002
<0.0106 0.058 0.02 O.0l4 5/07/2002
<0.0154 0.058 0.02 0.0 15 :
5/08/2002
<0.0150 0.056 0.019 5/09/2002
<0.0122 0.057 0.02 0.014 5/10/2002
<1.0189 0.056 0.019
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas and Ceriodaphnia dubia Tests, Sequoyah Nuclear Plant Effluent (SQN),
Outfall 101, May 5-14, 2002 Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Total Residual Test/
Initial Final Initial Final Initial Final Initial Chlorine Sample ID
(°C)
(oC)
(mg/L)
(mg/L)
S.U.
S.U.
(ptmhos) mg/L CaCO3 mg/L (mg/L)
CaC03 Fathead/
24.5 24.5 7.9 7.1 8.25 7.84 313 64 87 Control (24.3-24.6)
(24.3-24.9)
(7.8-8.0)
(6.4-7.7)
(8.23-8.27)
(7.54-8.10)
(298-321)
(63-65)
(82-89)
Fathead/
24.5 24.5 7.9 7.1 8.25 7.80 297 10.98%
(24.3-24.8)
(24.2-24.8)
(7.8-8.1)
(6.2-7.6)
(8.23-8.27)
(7.44-8.11)
(287-305)
Fathead!
24.6 24.5 8.0 6.9 8.23 7.76 281 22 %
(24.2-24.8)
(24.3-24.9)
(7.8-8.1)
(6.3-7.6)
(8.20-8.26)
(7.53-8.02)
(269-289)
Fathead/
24.5 24.5 8.1 7.1 8.19 7.84 250 43.9 %
(24.2-24.8)
(24.3-24.9)
(7.9-8.2)
(6.5-8.0)
(8.13-8.24)
(7.62-8.04)
(241-257)
Fathead/
24.6 24.5 8.1 7.0 8.15 7.79 211 72 %
(24.2-25.0)
(24.3-24.9)
(7.9-8.2)
(6.5-7.5)
(8.06-8.21)
(7.62-8.04)
(203-216)
Fathead/
24.6 24.5 8.2 7.2 8.08 7.80 170
- 58 75
<0.10 100 %
(24.3-25.1)
(24.2-24.9)
(8.0-8.4)
(6.5-7.6)
(7.93-8.18)
(7.65-8.04)
(165-175)
(57-59)
(72-76)
(<0.10-<0.10)
Fathead/
24.6 24.5 8.3 7.1 8.11 7.91 171 60 74
<0.10 Intake (24.4-25.1)
(24.2-24.9)
(8.1-8.4)
(6.6-7.7)
(7.99-8.18)
(7.67-8.22)
(165-173)
(58-61)
(72-76)
(<0.1 0-<0. 10)
Daphnid/
24.4 24.6 7.9 8.1 8.25 8.27 313 64 87 Control (24.2-24.7)
(24.4-24.9)
(7.8-8.0)
(7.9-8.4)
(8.23-8.27)
(8.18-8.38)
(298-321)
(63-65)
(82-89)
Daphnid/
24.6 24.6 7.9 8.0 8.25 8.27 297 10.98 %
(24.3-25.0)
(24.3-25.1)
(7.8-8.1)
(7.9-8.3)
(8.23-8.27)
(8.19-8.39)
(287-305)
Daphnid/
24.7 24.7 8.0 8.0 8.23 8.27 281 22 %
(24.3-25.1)
(24.3-25.1)
(7.8-8.1)
(7.9-8.3)
(8.20-8.26)
(8.18-8.38)
(269-289)
Daphnid/
24.7 24.7 8.1 8.1 8.19 8.26 250 43.9%
(24.4-25.1)
(24.4-25.1)
(7.9-8.2)
(7.9-8.4)
(8.13-8.24)
(8.18-8.37)
(241-257)
Daphnid/
24.8 24.7 8.1 8.1 8.15 8.30 211 72%
(24.4-25.3)
(24.4-25.0)
(7.9-8.2)
(7.9-8.3)
(8.06-8.21)
(8.18-8.42)
(203-216)
Daphnid/
24.8 24.6 8.2 8.2 8.08 8.33 170 58 75
<0.10 100 %
(24.3-25.4)
(24.4-25.1)
(8.0-8.4)
(8.0-8.4)
(7.93-8.18)
(8.18-8.50)
(165-175)
(57-59)
(72-76)
(<0.10-<0.10)
Daphnid/
24.6 24.6 8.3 8.2 8.11 8.27 171 60 74
<0.10 Intake (24.3-24.8)
(24.4-25.1)
(8.1-8.4)
(7.8-8.6)
(7.99-8.18)
(8.15-8.54)
(165-173)
(58-61)
(72-76)
(<0.10-<0.10)
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephales promelas Test, Sequoyah Nuclear Plant (SQN),
UV Treated Outfall 101 and Intake Samples, May 7-14, 2002 Temperature Dissolved Oxygen
_H_
Conductance Alkalinity Hardness Total Residual Test/
Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC)
(OC)
(mg/L)
(mg/L)
S.U.
S.U.
(irmhos) mg/L CaC0 3 mg/L (mg/L)
CaCO3 Fathead/
24.5 24.5 8.0 7.2 8.23 7.87 307 64 87 Control (24.3-24.7)
(24.3-24.8)
(7.9-8.2)
(6.5-7.8)
(8.18-8.27)
(7.49-8.12)
(296-315)
(63-65)
(82-89)
Fathead/
24.6 24.5 8.0 7.1 8.22 7.87 292 10.98 %
(24.3-24.8)
(24.2-24.8)
(7.9-8.2)
(6.1-7.8)
(8.17-8.28)
(7.48-8.12)
(287-300)
Fathead!
24.6 24.5 7.9 7.2 8.20 7.89 277 22 %
(24.3-25.0)
(24.2-24.8)
(7.7-8.1)
(6.5-7.8)
(8.16-8.25)
(7.45-8.10)
(270-283)
Fathead/
24.6 24.5 7.9 7.2 8.18 7.83 247 43.9 %
(24.3-25.1)
(24.2-24.8)
(7.7-8.2)
(6.4-7.7)
(8.12-8.24)
(7.43-8.10)
(240-253)
Fathead/
24.6 24.5 8.0 7.0 8.14 7.83 209 72 %
(24.3-25.2)
(24.2-24.8)
(7.8-8.1)
(6.0-7.7)
(8.08-8.20)
(7.42-8.08)
(204-214)
Fathead/
24.7 24.5 8.0 7.2 8.10 7.82 168 58 75
<0.10 100 %
(24.2-25.3)
(24.3-24.8)
(7.9-8.1)
(6.4-7.7)
(7.99-8.17)
(7.44-8.08)
(164-173)
(57-59)
(72-76)
(<0.10-<0.10)
Fathead/
24.6 24.5 8.0 7.1 8.10 7.84 168 60 74
<0.10 Intake (24.3-25.0)
(24.2-24.8)
(7.9-8.1)
(6.2-7.7)
(8.02-8.14)
(7.47-8.10)
(165-172)
(58-61)
(72-76)
(<0.10-<0.10)
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Project Name: TVA SQN P.O, Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: 0026450 Collected By: WANDA ALLEN 1I 1
Environmental Testing Solutions, LLC Research Building 1 Ecusta Road, Brevard, NC 28712 Phone: 828-862-8193 Fax: 828-862-8195 I
Samule Custodyv-Fill In From Tnn f-lnun Delivered By (Circle One):
SUPS Bus Client Other (specify):_
General Comments:
Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time ftA 4
bed4.&'e-----
sAA-is'° FeŽ ac°""z, tt*
r.
-)
I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for eael sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
!1 Page 1
of -1
BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Project Name: TVA SQN P.O. Number: P0 BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: 0026450 Collected By: WANDA ALLEN I
V Environmental Testing Solutions, LLC Research Building 1 Ecusta Road, Brevard, NC 28712 Phone: 828-862-8193 Fax: 828-862-8195 Delivered By (Circle One):
SUPS Bus Other (specify):
General Comments:
Container............
Field Identification I Grab/
Ship.
ColcinDt/ie Number &
Flow Rain Event?
Sample Description Comp.
Temp. (
Volume (MGD)
(Mark as Appropriate)
U.e
°C)
Collected S.........
Start End Y-If Yes, No Trace EST S..
f.Yes,.No.Trace T.Lag A..al......T.me
.ppear Inches A
5/08/02 A
SQN-101-TOX comp 4
5/7/02
//"s 2(2.5g) no am...90 0i C cJ\\ iNT~4it SQN-INT-TOX comp 4
5/7/02 5/08/02 1 (2.5g) no i ii0ii:i i
i I2.
e s S..........
i
- ;l jl t
- ' " < i i !* : :*i Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time Fee
-0x Client Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
Page 1
of -1
BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Project Name: TVA SQN P.O. Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: 0026450 Collected By: WANDA ALLEN U
Environmental Testing Solutions, LLC Research Building 1 Ecusta Road, Brevard, NC 28712 Phone: 828-862-8193 Fax: 828-862-8195 Delivered By (Circle One):
H UPS Bus Client Other (specify):_________
General Comments:
C ontainer Field Identification I Grab!
Ship.
Cleto aeTm Number &
Flow Rain Event?
Sample Description Comp.
Temp. (Volume (MGD)
(Mark as Appropriate) 0C)
Collected
.°.
C*)*C*o*lStart End o..::::i::::::
- .1nva1
- .I ::4.: i.y a:
SQN-101-TOX comp 4
5/9/02 2(2.5g) 7-j i*..
5/ 0 0
................ :S A W LC:
SQN-INT-TOX comp 4
T!579/02 5/10/02 (2,5iI) i..I...
______________________________________Sample Custody - Fill In From Top Down__________________
Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time d_---'
o ----oz s
s
.q Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for eacl sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
It Page I
of -1
Environmental Testing Solutions, LLC Sample Receipt Log Date Time Received Received Sample Project Sample Received Received By From Temperature Number Number Sample Name and Description State Comments 04_2502__
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Date Time Received Received Sample Project Sample Received Received By From Temperature Number Number Sample Name and Description State Comments
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Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promelas Client: T/VA Facility: Q* 31NAA tJc.*
PA,-JT" NPDES #: TN -COZ6(4S N3OM~T~eA-r0b Dilution Preparation info ration:
Comments:
M D i mS b a tch :
C o rm ber:
D i l u t i o n p b e ter e
n d %o_
I
¢T '
O r aue t s volum e (m L ):
Z A
a n
Diluent volume (mL) j r,'
17 2
Test organism information:
Test information:
Organism age:
2r-N-inutm OL-1b Randomizingtepae LIa'
[ D a t e a n d t i m e s o r g a n i s m s 0a s
- 06
-a 7.
tc*
.o q 0 0 )
A s
'r I n c u b a t o r n u m b e r :
Twere born between:
1Q30 - iuor k-s-v OOrganism source:
&-A OSL C*06-0"0L Artemia lot number:
ft-W,02 P
Transfer bowl information:
pH Temperature Oven temperature:
-11,AR Z.
0" C-Drying time:
.atno6 Daily feeding and renewal information:
Control information:
Acceptance criteria
% Mortality:
- 20%
Average weight pr larvae:
- 6. c/03
> 0.25 mg/larvae
'I I
Summary of test endpoints:
7-day LC50
'J 5'
NOEC I*
NOEC nr7.
LOEC ChV IC25 1C25 I >00%
=
]
Date: o*--'1 -0"7.
Survival and Growth Data Day CONTROL I0,q8%.
A B
C D
E F
G H
I J
K L
0
/0 10
/0
/0
/o
/0
/*
/6
/0 10
/0 /0
/0
/0
/6
/6
/0
/0 /o
/0
/0
/0 16
/0 2
/ /0
/D I/
10
/og'
/
/0
/0/0 Z4 110 1
/0
/0 10 1/
t 0
/0 to 10 10 (C) 1010
/0(10
£~to 10
/0 /0 6
10 to 1O 10 10 10 io
/o
'O /1 C
to 7
o I
q 4 1 0
/
/0/0 /0 A
/0 A= Pan weight (mg) wu
,..Pan+*Larvae weight(*mg)
.v
-10 Larvae weight (mg)= A-B qt*
1 4~2.
- k
' k.-
-J 6 0 Calculations and data reviewed:
Comments:
"&I A, UVA (
Species: Pimephales promelas Client: -'JA-r S&Jct.A N~o.ýýAe~b
0 0
2 CX Y
Species: Pimephales promelas Client:
-TV'Pr-S*_axl AA Date:
501-0 02.
Survival and Growth Data Day i
%7I* Ae*
- Y Z
AA BB 0
/0 10
/0
/0 I
/ D /0 JO
, 0 2
/0 I1) 0O /0 3
10 b )10 t 0
4 16
/a-10
/I/W(
6 o 10 6 6 10 2
A = Pan weight (mg) 14.d A B = Pan + Larvae weight (mg)
AD Larvae weight (mg)=A-B
__________(A._
0,55__
2S t 4-vo Calculations and data reviewed.4-Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated May 7-14, 2002 Project number:
324 Reveiwed by:
),,_
Outal101 Dunnett's MSD value:
PMSD:
Intake Dunnett's MSD value:
PMSD:
0.1116 25.3 0.1617 36.7 MSD =
Minimum Significant Difference PMSD Percent Minimun Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant differece occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7P from the control (detennined Lower PMSD bound determined by USEPA (10th pereentile) = 9.4%/
Upper PMSD bound determined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, Off.
Client:
Test dates:
Concentration Reeplcate Initial number of Final number of larvae A - Pan weight B - Pan + Larvae Larvae weight (rag)
Wei l
a
(
o Wegh /Intilamer11ý uvialMen eght (mmg coeffickat of Pamct reduction from larvae (mg) weight (mg)
- A - B of Larvae (mg)
(%)
variatio (%)
casrtl ('h)
A 10 10 14.936 19.420 4.484 0.4484 Control B
10 10 14.802 19.560 4.758 0.4758 97.5 0.4403 6.7 Not applicable C
10 10 14.888 18.940 4,052 0.4052 D
10 9
14.292 18.610 4.318 0.4318 E
10 10 15.039 20.660 5.621 0.5621 10.98%
F 10 8
14.904 18.510 3.606 0.3606 95.0 G
1 0
10 15.153 20.720 5.567 0.55679 0.4955 19.9
-12.5 H
1 10 10 14.676 19.700 5.024 0.5024 1
10 10 15.034 20.000 4.966 0.4966 22%
J 10 10 14.831 19.460 4.629 0.4629 95.0 0.4461 14.2
-1.3 K
10 8
15.037 18.570 3.533 0.3533 L
10 10 14.684 19.400 4.716 0.4716 M
10 10 15.127 20.960 5.833 0.5833 N
10 9
14.679 19.160 4.481 0.4481 97.5 0.4974 16.9
-13.0 0
10 10 15.090 19.150 4.060 0.4060 P
10 10 14.587 20.1 10 5.523 0.5523 Q
10 10 15.134 19.340 4.206 0.4206 72%
R 10 10 15.436 20.300 4.864 0.4864 97.5 0.4832 S
10 10 15.091 20.280 5.189 0.5189 9.1
-9.7 T
10 9
14.971 20.040 5.069 0.5069 U
9 9
14.788 19.700 4.912 0.5458 100%
V 10 10 15.019 21.260 6.241 0.6241 W
10 10 14.791 19.710 4.919 0.4919 97.5 0.5494 10.0
-24.8 X
10 9
15.091 20.450 5.359 0.5359 Y
10 10 14.621 19.400 4.779 0.4779 100% Intake Z
10 7
14.681 18.570 3.889 0.3889 67.5 0.3639 45.0 17.4 AA 1
0 2
15.007 16.260 1.253 0.1253 BB 1
0 8
14.857 19.490 4.633 0.4633
Environmental Testing Solutions, LLC Statistical Analyses Laval Mh Gratb and Surviv Tat-7 Day QGmh S-tar 5,V7102 Tat 11:.
PpFRCR Sample a).
TVA. Seqo
.- Noeseod End Dam 5114102 La ID:.
ErS-Ewv. TUVaS Solua Sample Type:
DMR-DIkd-Monimei; Report Sample D"M Pronoad EPAF 91-EPA Frhwsar Ta: Species:
pp-piql p-d Commens..P-i~hsm*m~
Cone-%
1 2
3 4
D-Contol 0.4484 0.4758 0.4052 0.4318 10.98 0.5621 0.3606 0.5567 0.5024 22 0.4966 0.4629 0.3533 0.4716 43.9 0.5833 0.4481 0.4060 0.5523 72 0.4206 0.4864 0.5189 O.,069 100 0.5458 0.6241 0.4919 0.5359 Transform: Untramsformed 1-Tailed Isoak Cooc-%
Mean N-Mean Mean Mn Max CV%
N 1.-Stat Critcal MSD Mean N-Msar D-Coorol 0.4403 1.000 0.4403 0.4052 0.4758 6.725 4
0.4853 1.0000 10.9a 0.4955 1.1253 0.4955 0.3606 0.5621 18.944 4
-1.191 2.410 0.1116 0.4833 1.0000 22 0.4461 1.0132 0.4461 0.3533 0.4966 14.233 4
-0.12M 2.410 0.116 0.4853 1.0000 43.9 0.4974 1.1297 0.4974 0.4060 0.5833 16.891 4
-1.234 2.410 0.1116 0.4853 L.O00 72 0.4632 1.0974 0.4832 0.4206 0.5189 9.072 4
-0.927 2.410 0,1116 0.4853 1.0000 100 0.5494 1.2478 0.5494 0.4919 0.6241 10.014 4
-2.357 2.410 0.1A16 0.4853 1.0000 Axillazy Tests Statistic Critical Skew Kert Shapie-Wiik's Tes adian normaJ diseibceoe (p > 0.01) 0 9559744 0.884
-0.639853048
-0.108351025 Bsgleas Teai dicate equal v.nar (p - 0.52) 4.198050499 15.08631706 Hypothesis Teat (1-tail 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-4Prob df Dunmnts Test 100
>100 I
0.111504903 0.253429259 0.006341912 0.004287526 0.245395169 5,10 Ueeear Interpolalon (SO Ressmples)
Point SD 95% CL(Exp)
Skew IC05
>100
[CIO
>100 ICI5
>100 1.0 IC20
>100 0.9 0:
>100 0.0 1C40
>100 0.7 IC50
>100 0.6 0.5 o
0.4 C *'0.3.
02 e 0.1 0.0
.1
-0.2
-0.3
-0.4 0
20 40 60 80 100 120 Dose %
Dose-Response Plot 0.7 0.6 0.5 S0.4 0.3.......................................................1-tail.
0.05 level
- 0.
of significance I,
sqnOS-07-O2data.xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date:
5/7/02 Test ID:
PpFRCR Sample ID:
TVA, Sequoyah - Nontuated Intake End Date:
5/14/02 Lab 1D:
ETS-Env. Testing Solutions Sample Type:
DMR.-Discharge Monitoring Report Sample Date:
Protocol:
EPAF 9 1-EPA Freshwater Test Species:
PP-Pimephales promeas Coro*smes:
Cone-%
1 2
3 4
D-Control 1.0000 1.0000 1.0000 0.9000 100 1.0000 0.7000 0.2000 0.8000 Transform: Arcsin Square Root 1-Tailed Conc-%
Mean N-Mean Mean MmN Max CV%
N t-Stat Critical mSD D-Contol 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4
100 0.6750 0.6923 0.9935 0.4636 1.4120 39.790 4
1.872 1.943 0.3922 Auxiliary Tests Statistic Critical Skew Knrt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.880755126 0.749
-0.780447334 2.82895682 F-Test indicates equal variances (p = 0.03) 23.53577423 47.46834564 Hypothesis Test (l-tall, 0.05)
Homoscedastic t Test indicates no significant differences sqnOS-07-O2data~xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Stan Date:
5/7/02 Test ED:
PpFRCR Sample ED:
End Date:
5/14/02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
Sample Date:
Protocol:
EPAF 91 -EPA Freshwater Test Species:
Comments:
Conc-%
1 2
3 4
D-Control 0.4484 0.4758 0.4052 0.4318 100 0.4779 0.3889 0.1253 0.4633 Transform: Untranslformed Conc-%
Mean N-Mean Mean Min Max CV%
N D-Concrol 0.4403 1.0000 0.4403 0.4052 0.4758 6.725 4
100 0.3639 0.8264 0.3639 0.1253 0.4779 45.002 4
TVA, Sequoyah - Nonovated intake DMR-Dischargc Monitoring Report PP-Pimephales promelas I-Tailed t-Stat Critical MSD 0.919 1.943 0.1617 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.842814147 0.749
-1,634364624 3.644213996 F-Test indicates equal variances (p = 0.02) 30.58085251 47.46834564 Hypothesis Test (I-tail, 0.05)
Homoscedastic t Test indicates no significant differences sqnO5-07-O2data.xfs
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1002.0)
Species: Ceriodaphnia dubia Time Analyst Client: VA Se
- oa*
Facility:
NPDES #: "V4 - oQ M*sa Dilution preparation in ormation:
Comments:
Diuton prep (%)
Itom~ I ~- 7-~
1oa twk Effiuenvol.ume(mL) IL-00 It I
q
,0 Diluentvolume(mL) t&.5 11-0 1ql.5!
q.o o
Test organism information:
Test information:
Organism age:
-<2A-- t'C046 OLU Randomizing template:
AMJ-t.
Date and times organisms 1-%% -t O 'Tat-Incubator number:
were born between:
I O rgani sm source:
0q-U.03 2
Me 6*
Y C T batch:
A SS,
t.- Ota-7 Transfer bowl information:
pH = t. 25 Temperature= z4.1 Selenastrum batch: AM 1-16I-02.
CONTROL Survival and Reproduction Data Replicate number
- Day, 1
2 3
4 5
6 7
8 9
10 1
I Young produced C:
0 0
0 Adult mortalty t;[
9
- 10.
2 Young produced r
r)
OO Adult mortality I-, _..."
[A d u ungm p rod uced [ '4 H
C Adult mortality C
4 Young produced 0
I5 IQ 7 -IA Adult mortality i
5 Young produced ID 10 1
o Adult mortality I 1-1 L-V'I L
~
L.~
L..
6 Young produced
-L IZ
.2 1
Adult mortality j.I P P I 1
73 Young produced C) 0, I '
C 0
-0 0
C)
Total young produced 2s e
2-q z,
2-5 2
2 31 Final Adult Mortality 7--*
X for 3` Broods Calculations and data reviewed:
Test Renewal, Feeding, and Incubator Location Information Mon Tue Wed Thu Fri Sat Sun Date 054 Time m
I' M
l 2
16__
L=
Analyst Shelf 1
.62.
Location Control information:
Acceptance criteria Summary of test endpoints:
% of Male Adults:
- 5 20%
7-day LC50
>)M
% Adults having 3rd Broods:
I02'.
> 80%
NOEC I Vr.
% Mortality:
M
< 20%
LOEC
"-100%
Mean Offspring/Female:
2".6
> 15.0 offspring/female ChV
%CV:
go/'*
<40.0%
IC25
>10M____
Date Test start Test end os.o--'oI z 10 I m4 OS-tq02, IIsw l
Species: Ceriodaphnia dubia Client: TVI,- SeeQ>%ŽNA CONCENTRATION: W).A9%
Date:
(s-o1-oZ..
Replcate number Da y o
3 r o6 7
e 9
10 Adult mortality C)
C) 0
~
2 Young producedC 0
C Adult mortality t
[
U 3
Young produced a
4
[
Adult mortality L.
.- T U
4oYoung produced qra Adult mortality t
5 Young produced 10
_I?
Adu n
itmortality JL U-.
I L-j XL k-4 k6 Young produced 1ja9j{Ij lT0 i~
Adult mortality L
Red utl Su.viv=al an Dt 7
Young produced I 405a 2 )
n8 I Z
29
'I 3I I-zC) 2__
Total aon aroaucea i a1 2-a~
a3a Final Adult Mo rtali ty Concentration:
A r%
Mortality:
m0yI Mean Offspring/Fexnale:
eo
% Reduction from Control:
- 3 Calculations and data reviewed:
CONCENTRATION:
Survival and Reproduction Data Day 2
3 ~~~
Replicate nnumber Day 1_____3_4 5
1 6
7 8
9 10 Adult mo__a l__y AdAutmortality L
.U.
i g 2 Young produced 0
C C
0 0
O 2_talYoung produced cedk[1 2 I0 Fin A
dAdul t mortality
_ _ _._l_
Young p roduced o
q 5Ic VF7 oW 3TL-1 Adult mortality
~
~ Il ? t-.
__T JT j~
5 Young produced I T10 1
T 4s" P
T~
Adult mortality I_ U 7
7 L-
__ L LL-K 1:7-t.
6 Young produced LLIi.J IsI 9 q
'0 1 E Adult mortality [LI L-FN
-_~tI~I 6
Young produced 0~~
-0 1-1 Z5 Total young produced
- ?o-9 at.0Z725 P
Final Adult Mortality_
tZo CT L-L,17-L t 1L Concentration:
% Mortality:
0070 Mean Offspring/Female:
2k.,5
% Reduction from Control: "3. 3.
Calculations and-data reviewed:
Species: Ceriodaphnia dubia Client: -TVA - See,-q,.
1AA CONCENTRATION:
4_V Date: oS-ot1 -o-4.
Surivl ndRe~odctonData Da Replicte number Da 1
2
- 3 4
5 6
7 8
9 10 1
Y o u png pr d u e o I C )
o Z
0 6 (>
63 6*
2 Young produced 0"
0 0
0-(
Q Adult mortality t--
LT I
t.
"2 Young produced AI
- 4 C)
-' T 4
_onpou__U 0
c) 5 Young produced 1
d r_"._
-inal Adult mortality L
I.-
T L
6 Youngoproduced Ise IS
)An Aultmortality M rtly L
1 1 5
Young produced Opg l
1A, 03 Adut oralty L.t
.~%
Reucio fro Conrol 6oa Young produced
.L Fn Adult mortality L
K -_7 -. L..
I
,,,-mr
-- I
.. I
.- I L-
- m.
Total young produced r
T
-*0
- 2.
='
Final Adult Mortality
[ T?
?
- ?
- -t
- .Z
-Concentration:
%Mortality:
Mean Offspring/Female:
25i, 0
% Reduction from Control: -,S,% %
Calculations and data reviewed:
CONCENTRATION:
I
%Survival and Reproduction Data I _____
Replicate number Day_
1 1
2 1
3 1
4 5
6 7
8 9
10 A utmortality t1_
2 Young pro~duced C)
C_
010 C
3 Young produced 1_
0-4 1
\\__3 I) 1..
0QC Adult mortality
_____i__
Adultimortality L
L 5
Young produced I#
7_ 1z 10
/0~& 10 1?-
=
IAdult mortality I
L_L4 l _
1 LU I
_ I L_
L_
L-t-
6 Yung produced C
__1_
1 0 1__
1 ICA.I I
11 Adul mortality 1____
.I I 4
'7 7
Young produced 6
L Total young produced Z-7-6 Zce 0
2-1 _31 Z1 I -1
- 30 3 Z 3'-1 Final Adult Mortality jTT7(\\
~
~~
Concentration:
I% Mortality:
00f.
-Mean Offspring/Female:
Zq, I
% Reduction from Control:
Calculations and data reviewed:
Species: Ceriodaphnia dubia Client: TVA - Sea-A*
k CONCENTRATION: Jb'01 Date:
OS-0"-...
Survival and Rflhz" Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
Young produced
._01
- 0.
0 0
0 2
Young produced [ '4 O
I O AYoung produced C_ )---
=
I Adult mortality
,U.
-- m,,
I
- I1 0
Y Young produced 07>
0o o
Adult mortality 1
' 1 t
L 10J L.1L A
5 Youngoproduced C
Aent Adult mortality ai t 7nYoung produced O-7 fj:4
-C-Total young produced
~
~
3
~
3 S
3 L
Final Adult Mortality
-T 17 Concentration:
% Reduction from Control:
- tZ.0. */
Calcutlations and data reviewed:_.
CONCENTRATION: I C).*
Survival and Reproduction Data Replicate number Day 1
2 3
4 5
6 7
8 9
10 1
Young produced 0
6 C)
I 0
0 I
ý)
iAdult morat L-7 L
Adult mortality 3
[Young produced A4 0 _
I I0]
01 I
7XI3 0
[Adult mortali'ty C
1 7 1
j]k I
1
~
4 Young produced t.4 5
-5 C
F Adult mortaltyL C_
5 Young produced 10__1 6
Young produced 1IC)1 0i~ 0 17-K?
t4Q i+/-7 IAdult mortality] (-I t.-
IL L_]LL-4L 7 Young produced A
..L.,~
V
- 0.
10 0
0 Toalyongprdued2Jz -Zj 15 1'4 123 le~ 240a Final Adult Mortality j
~
jU]T
~
Concentration:
I% Mortality:
I Mean Offspring/Female:
ZZ., 0
% Reduction from Control:
ZO.%
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, Nontreated May 7-14, 2002 Project number:
324 Outfail 101 Dunnett's MSD value:
PMSD:
Intake:
Dunnett's MSD value:
PMSD:
2.593 9.4 3.272 11.9 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 12.6% from the control.
Lower PMSD bound determined by USEPA (I0Oh percentile) = 11%.
Upper PMSD bound determined by USEPA (9 0dt percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceo hnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Client:
Test dates:
Revelwed by:
Concentration Replicate number Survival Average reproduction Coemdent of Percent reduction from
(%)
1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variation (%)
control (%)
Control 25 28 31 32 24 26 25 25 29 31 100 27.6 10.8 Not applicable 10.98%
26 28 28 29 29 31 27 26 32 30 100 28.6 7.0
-3.6 22%
26 31 26 32 29 31 30 27 25 28 100 2&5 8.6
-3.3 43.9%
25 28 26 31 29 34 28 28 30 31 100 29.0 9.1
-5.1 72%
27 26 30 27 31 27 27 30 32 34 100 29.1 9.2
-5.4 100%
30 31 28 30 28 29 33 35 33 32 100 30.9 7.5
-12.0 100% Intake 30 22 24 21 15 14 23 18 26 27 100 22.0 23.5 20.3
Environmental Testing Solutions, LLC Statistical Analyses Cerodaphnia Survival and Reproduction Test-Reproduction Start Dae:.
5f7/02 Test ID:
CdFRCR Sample ID:
TVA, Sequoyah - Nowreated End Date:
5114/02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
DMR-Discleargo M toring Report Sample Daze:
Protocol:
EPAF 91 -EPA Freshwater Test Species:
CD-C a
dubia Comment:
Conc-%
1 2
3 4
5 6
7 3
9 10 D-Coonol 25.000 28.000 31.000 32.000 24.000 26.000716X25.000 25.000 29.000 31.000 10.98 26.000 28.000 28.000 29.000 29.000 31.000 27.000 26.000 32.000 30.000 22 26.000 31.000 26.000 32.000 29.000 31.000 30.000 27.000 25.000 28.000 43.9 25.000 28.000 26.000 31.000 29.000 34.000 28.000 28.000 30.000 31.000 72 27.000 26.000 30.000 27.000 31.000 27.000 27.000 30.000 32.000 34.000 100 30.000 31.000 28.000 30.000 28.000 29.000 33.000 35.000 33.000 32.000 Tranform: Untransformed l-Taled hotoiak Conc-%
Mean N-Moan Mean Min Max CV%
N t-Stat Critical MSD Mean N-MeaM D-Contol 27.600 1.0000 27.600 24.000 32.000 10.829 10 28.950 1.0000 10.98 28.600 1.0362 28.600 26.000 32.000 7.032 10
-0.882 2.287 2.593 28.950 1.0000 22 28.500 1.0326 28.500 25.000 32.000 8.634 10
-0.794 2.287 2.593 28.950 3.0000 43.9 29.000 1.0507 29.000 25.000 34.000 9.051 10
-1.235 2-287 2.593 28.950 1.0000 72 29.100 1.0543 29.100 26.000 34.000 9.228 10
-1.323 2.287 2.593 28.950 1.0000 100 30.900 1.1196 30.900 28.000 35.000 7.544 10
-2.911 2.287 2.593 28.950 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolnogorov D Test indicates normal distrbution (p > 0.0 I) 0.83713907 1.035 0.298146516
-0.9893687 Bar.let's Test indicates equal variances (p 0.9 1) 1.532001376 15.08631706 Hypothesis Test (1-tail, 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dumnett's Test 100
>100 1
2.592676189 0.093937543 11.95 6.427777778 0.136908096 5,54 Linear Interpolation (80 Resamples)
Point SD 95% CL Skew IC05
>100 ICI0
>100 ICI5
>100 1.0 1C20
>100 IC25
>100 0.8 IC40
>100 0.8 IC50
>100 0.7 0.6 0.5 0.4 0 0.3 0.2 0.1 0.0 e
o o.1 _. *..
-0.1
-0.2 0
20 40 60 80 100 120 Dose %
Dose-Response Plot 40 35 o25-1-tail, 0.05 level
.2 2of significance 20 15 10 5
0 CD o;
os 05 C-1 sqnO5-07-O2data.xls
Environmental Testing Solutions, LLC Statistical Analyses Ceriodaphnla Survival and Reproduction Test-Reproduc*_on Start Date:
517/02 Test ID:
CdFRCR Sample 0D:
TVA, Sequoyah - Nontrea*ed End Dam.
5/14/02 Lab UD:
ETS-Env. Testing Solutions Sample Type:
DMR-DIucharge Monitoring Report Sample Date:
Protocol:
EPAF 91-EPA Freshwater Test Species:
CD-Ceiodaphnaa dubia Comments:
Conc-%
I 2
3 4
5 6
7 9
10 D-Coitrol 25.000 28.000 31.000 32.000 24.000 26.000 25.000 25.000 29.000 31.000 100 30.000 22.000 24.000 21.000 15.000 14.000 23.000 18.000 26.000 27.000 Transform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD D-Control 27.600 1.0000 27.600 24.000 32.000 10.829 10 1100 22.000 0.7971 22.000 14.000 30.000 23.473 10 2.968 1.734 3.272 Audllary Tests Statistic Critical Skew Kurt Shapiro-Wilkls Test indicates normal distribution (p > 0.01) 0.983128428 0.868
-0.124038982 -0.311539277 F-Test indicates equal variances (p = 0.12) 2.98507452 6.54108572 Hypothesis Test (I-taiL 0.05)
Homoscedastic t Test indicates significant differences sqnO5-07-O2dataxLs
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promelas Client: "rV A Facility: -F-MAiA M*
- 4 r"
NPDES #: TN-O*O*24,S) o9-*
A-Teb Dilution preparation information:
Comments:
MHS batch:
O 0-oz-It" Dilution prcp(I%)t
-z7-c46.41
-1z on~
2 ýtu_&.e Oý.
Effluent volune (miL) ~
J.
Ic Test organism information:
Test information:
Organism age:
.q-AoUeL.
0OLb Randomizing template:
'IeUzou)
Date and times organisms CS-06-O2 IoW -To 1400 M.ST7-Incubator number:
were born between:
t 9.I t koo C-c Organism source:
A&,6ATcA os-o6-62.
Artemia lot number:
&*'oz01f Transfer bowl information:
pH =
Temperature =
Oven temperature:
(,w
"".___2__,
0"*C Drying time:
Z4.
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time o0 ce--Oz 00, oo, I
C6 aCZ 1~q
)(all 15/-3 2
oG-o0
-a
/000 1&o2.
/&0 Z 3__
05_____0 OqS,3 IL04 Nzz.
6 OG I____-
1 HO t*,
4q-
.4 6
7
-oz-1__
Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
I 2. -s°7.
- 5 20%
7-day LC50 Average weight per larvae:
O.q"-1
>0.25 mo/larvae NOEC IC25 LOEC
Species: Pimephales promelas Client:
hI" A-GeWMA Date:
O-O1-O'7 Survival and Growth Data Day CONTROL
-O.
-Z*
A B
C D
E F
G H
I J
K L
0 I
/o 10 /0 10
/0- /610 160 S/0l 10 0/0
/6
/0 16
/0
/0 /01 /010 2
11/0/0/0/0
/0/0/
/0
/
/0 3
/0
/C)
/0 /
I0I/0 o
O 10
!o 6
4 I/0 0
0 10 t'O 10 0 t
01
/0
/D to
/0
/
0
/0 10 10
/0
/0
/0 lb 7
/
f e
6 IO
/0 q
0
/6O 10
/0 A =P a ri I 0
\\
a B~
Pan
+ Larvae weight (mg) 0 1111%
02 Calculations and data reviewed:
J r
Comments:
Species: Pimephales promelas Client:
"T'* A - Sesoxg A K u N- -T4qetr>
Survival and Growth Data Day 1,
-2%.7
-orff.
_____M N
0 P
Q R
S T
U V
IW X
0 0
L
/0/
10
/0
/0/0
/0
/0
/0/0
/0 1
/010 0 /0 010
/4 I*
0
/0'
/0 2
/0
/0 10 0
0/
/0 /0
/O /c
/0
/0 3
10
'(0 '1 to lo t
01 1 I01 10 10 10 0Q 4
/0 I0 Cb t
10 10 10 10 10 10 10 10 10 5_/a 01c_
/0 1o 10 10 10 tO tO 0
1o 10 0
Q(
6 A
to to 10 Io to )o o /o 1 o io 10 q
0 7
/0
/)
10A
/0
/0
/101 l 16 10 A
=Pan w eight ( mng) o v 4 6 V O M
BPan + Larvae weight (rng)
- 70f4 o.b Larvae weight (ig) = A-B Calculations and data reviewed:
Comments:
Date:
05- 01-o-.
Species: Pimephales promelas Client:
TV A-Seno'iAA4
%- Tje-Xe~tý-b Date:
O's1c" -
Survival and Growth Data Day iO*Ot, J 'AI(e Y
Z AA BB 0
/0 10
/0
/0
/0
/0
/0//0 2
/0
/0
/0
/0 3
1O 0 Io JO (010 10 0
4j JO ic(
ID 5
to 10 16 1C0 6
10 1(3
/0 Io 7
/0 Ito I /0
/ 01 C2alculations and data reviewed:
Comments:
I
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses TVA Sequoyah Nuclear Plant, UV Treated May 7-14, 2002 324 Revelwed by: A L
Dunnelt's MSD value:
PMSD:
Dunnett's MSD value:
PMSD:
0.0747 15.8 0.0333 7.0 MSD =
Minimum Significant Difference PMSD -
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum pereent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control (deternined through Lower PMSD bound determined by USEPA (10th percentile) = 9.4/.
Upper PMSD bound determined by USEPA (90th pereentile) = 35%.
"The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Client:
Test dates:
Project number:
Concentration (%)
Replicate Initial anrber of Finl nuolieroftlrvae A - Po weight B - Pan + Larvae Larvae weight (rma Weight / Initial nauber Mean survival Mean weight (WOg)
CoPadct Of ar.t redactdiea ho larvae (rag) weight (ing)
A - B of larvae (ag)
(%)
vartatim (%)
comtrol (%)
A 10 10 14.632 19.570 4.938 0.4938 Control B
10 10 14.634 19.090 4.456 0.4456 97.5 0.4734 4.3 Not applicable C
10 9
14.701 19.450 4.749 0.4749 D
10 10 14.656 19.450 4.794 0.4794 E
10 9
15.020 18.790 3.770 0.3770 10.98%
F 10 10 15.037 19.810 4.773 0.4773 95.0 0.4194 17.8 11.4 G
10 10 14.947 19.820 4.873 0.4873 If 10 9
14.870 18.230 3.360 0.3360 1
10 10 15.173 20.210 5.037 0.5037 22%
J 10 10 15.004 19.840 4.836 0.4836 100.0 0.5092 4.7
-7.5 K
10 10 15.036 20.120 5.084 0.5084 11 10 10 14.791 20.200 5.409 0.5409 M
10 10 14.875 19.990 5.115 0.5115 43.9%
N 10 10 15.060 20.220 5.160 0.5160 100.0 0.5333 10.2
-12.6 0
10 10 15.145 20.070 4.925 0.4925 P
10 10 15.008 21.140 6.132 0.6132 Q
10 10 15.362 19.940 4.578 0.4578 72%
R 10 10 14.972 19.770 4.798 0.4798 100.0 0.4553 4.0 3.8 S
10 10 14.669 19.070 4.401 0.4401 T
10 10 14.787 19.220 4.433 0.4433 U
10 10 14.615 19.350 4.735 0.4735 100%
V 10 10 14.759 19.560 4.801 0.4801 97.5 0.4734 8.7 0.0 W
10 9
14.933 19.130 4.197 0.4197 X
10 10 15.186 20.390 5.204 0.5204 Y
10 10 15.015 19.780 4.765 0.4765 100% Intake Z
10 10 14.843 19.200 4.357 0.4357 100.0 0.4638 6.0 2.0 AA 10 10 14.982 19.450 4.468 0.4468 BB 10 I 0 15.067 20.030 4.963 0.4963 I
I I
I
)'l
Environmental Testing Solutions, LLC Statistical Analyses Lm-ral Fhh Growth and Survival Test-7 Day Growth Start Daue:
5/7/02 Test ID:
PpFRCR Sample ID:
TVA, Sequoyah - UV-,aated End Date:
5/14/02 Lab ID:
ETS-Eav. Testing Solutions Sample Type.
DMR-Diaharge Monitortg Repor Sample Date:
Protocol:
EPAF 9 I-EPA P water Test Species:
PP-Pihephtles Prontlas Commets:
Conc"-%
1 2
3 4
D-Conrol 0.4938 0.4456 0.4749 0.4794 10.98 0.3770 0.4773 0.4873 0.3360 22 0.5037 0A836 0.5084 05409 43.9 0.5115 0.5160 0.4925 0.6132 72 0.4578 0.4798 0.4401 0.4433 100 0.4735 0.4801 0.4197 0.5204 Transform: Untransformed I-Tailed haotonk Conc-%
Mean N-Mean Mean Min Max CV%
N t-Star Critical MSD Mean N-Meu D-Control 0.4734 1.0000 0.4734 0.4456 0.4938 4.272 4
0.4838 1.0000 10.98 0.4194 0.8859 0.4 194 0.3360 0.4873 17.798 4
1.743 2.410 0.0747 0.4838 1.00o0 22 0.5092 1.0755 0.5092 0.4836 0.5409 4.663 4
-1.153 2.410 0.0747 0.4838 1.0000 43.9 0.5333 1.1265 0.5333 0.4925 0.6132 10.169 4
-1.932 2.410 0.0747 0.4838 1.0000 72 0.4553 0.9616 0.4553 0.4401 0.4798 3.973 4
0.586 2.410 0.0747 0.4643 0.9597 100 0.4734 1.0000 0.4734 0.4197 0.5204 8.741 4
0.000 2.410 0.0747 0.4643 0.9597 Auxillary Tests Statistic Critical Skew KUnrt Shapiro-Wilk's Test indicates normal disatibution (p > 0.01) 0.980244517 0.884 0.193884967 0.15776262 Bardtets Test indicates equal variatces (p
- 0. 14) 8.409856796 15.08631706 Hypothesis Test (I-tail, 0.08)
NOEC LOEC ChV TU MSDu MSDp MSH MSE F-Prob df Dunno's Test 100
>100 1
0.074689285 0.157763711 0.00641525 0.001920934 0.026198762 5,18 Linear Interpolation (80 Resamples)
Point SD 95% CLExp)
Skew IC05
>100 ICIO
>100 ICIS
>100 1.0
[C20
>100 0.9 IC25
>100 0.8 IC40
>100 IC50
>100 0.7 0.6 0.5
& 0.4 S0.3 0.2 0.1
-0.1
-0.2
,- 0.2 1.
0 20 40 60 80 100 120 Dose %
Dose-Response Plot 0.7 0.6 0.5 04 1-tail, 0.05 level
- 04 of significance Ct.
0 Ct 0
Sr D
N 0
0 sqn_05-07-02data.xis
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
5/7102 Test ID:
PpFRCR Sample ID:
TVA, Sequoyah - UV-Treted Intake End Date:
5/14/02 Lab ID:
ETS-Ean. Testing Solutions Sample Type:
DMR-Dvis ge Monioring Report Sample Daze:
Protocol:
EPAF 9 I-EPA Freshwater Test Species:
PP-Pinephales promelas Comments:
Conc-%
1 2
3 4
D-Control 0A938 0.4456 0.4749 0.4794 100 0.4765 0.4357 0.4468 0.4963 Trnstform: Untransformed 1-Tailed Conc-%
Mean N-Mean Mean Mn Max CV%
N t-Stat Critical MSD D-Coatrol 0.4734 1.0000 0.4734 0.4456 0.4938 4.272 4
100 0.4638 0.9797 0.4638 0.4357 0.4963 5.965 4
0.560 1.943 0.0333 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicateu normal distribution (p > 0.01) 0.93363601 0.749
-0.063500364
-1.353307 16 F-Test indicates equal variances (p = 0.62) 1.070973468 47.46834564 Hypothesis Test Il-tail, 0.05)
Homnoscedastc t Test indicates no significant differences sqnOs-07-O2data.xLs
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Methods 1000.0 and 1002.0)
Species: Ceriodaphnia dubia and Pimephales promelas Summary of Chemical Analyses Client:
Sequoyah Nuclear Plant Non-Treated Test dates:
May 07-14, 2002 Project number:
324 Pimephales promelas Reviewed by:,
Ceriodaphnia dubia 24.2
- 4.
0.3 24.41 25.1 24.61 0.21
_43
- 2.
24..6
- 0.
24.21 L244.9 24.5 0.2 24.4 251 24.6 0.3 Overall Temperature (C)
(including all concentrations for initial and final temperatures) 24.2 25.1 24.5 0.2 24.2 25.4 24.7 0.3
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Parameter
___________I.
I pH (SU)
DO (mglL)
Conductivity (jumhbo/cm)
Alkalinity (mg/L CaCO3)
Hardness (mg/L CaCO,)
N
..'-..
I Initial Final lmtiai 7Initial 7
Final Initial 91 8.27 7.901
- 8.
6 ?:
.41 7.9 321 63
_Temperature CC) 244 243 246 246 24.5 24.51 246 24[31 249 24.4 24.5 24.3 pH826 811 824 766 8.23 7.91 827 7
8.24 7.841 8.24 DO (mg7L) 7 8.0 8.0 10.98%
Conductivity (pmhos/cm) 299 299 305
)2 22%
Conductivity 0tmhos/cm)282
__________697:28 Temperature CC) 246 2432 24.6 24.6 24 62 24.5 24.5 24.2 24.4 823H (SU)804 822 766 8.3 7
8.24 8.1 8.21 7.95 DO (mg7l)6 79 6
8.0 81 7
81
- 8.
7 8.2 22%
Conductivity (pmhos/cm) 257 25 28 289 241 244 282
'Temperature CC) 246 24.3 245 6
24.6 24.6 246 24.5 24.5 24.2 8H (SU)804 82 2
8.06 7.86 82 810 8.0 7
8.1 7
DO (mg7L)5 79 8.0 7.4 81 82 6
8.2 8.2 724.%
Conductivity (tmhos/cm) 212 21:
25_
241 244 Temperature CC) 24.8 24.3 24.5 24.6 24.6 26
- 24.
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.1 75 79
- 6.
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__L]
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<.01 2.1 2.1 2
Temperature (CC)24r 2.1 2.1 2,1 2.1 2.J 247 243 211 49 44 8H (SU)7.99 8.14 7.69 7.99 7.96 8.1 8.10 7.67 8.05 7.98 8.16 8.2 C1 nducainity (pmhg /cm)
I0 100% Intake AlkaHlnty (m9/L C*CO3) 60 56 Hardnes (mg/L CaCO3) 7
[7 iTotal Resildual Chlorine (ragL)
U0<.0F<01 Client:
Sequoyah Nuclear Plant Non-Treated Test dates:
May 07-14, 2002 Project number:
324 Concentration Control Reviewed by(4 m I I
Im Day 4 1
Day 5 1
Day 6 I
1 Final I Initial I
Final I Initial I
Fna
.271 7.671 8.251 7.861 8.25
.,i 7.8 6.6 8.0 7.2 8.0
- 6.
89 308
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Daily Chemical Analyses
- nt:
Sequoyah Nuclear Plant Non-Treated t dates:
May 07-14, 2002 ject number: 324 mcentration Parameter Day 0 Day I Day 2 Day 3 Day 4 Day 5 Day 6 Inta inal I I nta IFil Iiil Final I
niilIFnl Initial I
Final
!Initial Fial Intal Fina pH (SU) 8.27 8.26 8.23 8.38 8.24 8.26 8.27 8.26 8.27 8.18 8.25 8.28 8.25 8.30 DO (mg/L) 7.9 8.1 7.8 7.9 7.9 8.3 7.9 8.1 7.8 7.9 8.0 8.0 8.0 8.4 Conductivity (jtmhos/cm) 319 3142 298 308 Control Alkalinity (mg/L CaCO 3) 641 65 Hardness (mgfL CaCO3)
E 89 82 Temperature (CC) 24.3 24.5 24.6 24.9 24.3 24.7 24.7 24.4 24.8 24.5 24.7 24.2 24.4 pH (SU) 8,26 8.20 8.24 8.39 8.23 8.25 8.27 8.2 8.24 8.19 8.24 8.30 8.24 DO (mg[L) 8.1 7.9 7.8 7.9 7.9 8.3 7.9 8.0 7.8 7.9 8.0 8.0 8.0 8.2 10.98%
Conductivity (jmhos/cm) 30-1-'-
29-----0 2--
28-297 Temperature (*C) 24.6 24.3 24.7 25.0 24.6 24.7 24.74 24.4 25.0 25.1 24.6 24.6 24.3 pH (SU) 8.26 8.23 8.24 8.8 8.20 8.25 8.26 8.24 8.21 8.18 8.21 8.31 8.24 8.29 DO (mg/L) 8.1 7.9 7.8 7.9 8.0 8.2 8.0 8.0 8.1 7.91 8.0 8.1 8.1 22%
Conductivity (pmhosIcm) 287 2828--5269 273 282 Temperature (CC) 24.8
- 24. P 24.7 25.1 24.6 24.7 24.8 24.4 25.1 25.1 24.6 24.6 24.3 24.4 pH (SU) 8.23 822 8.22 8.34 8.13 8.25 8.24 8.20 8.16 8.18 8.15 8.3 8.21 8.26 DO (mg/L) 8.2 7.9 7.9 80 8.0 8.2 8.1 8.0 8.1 7.9 8.1 8.3 8.2 8.4 Conductivity (pimhos/cm) 254 253 252 257 241 244 250 Temperature (CC) 24.8; 24.5 24.6 25.1 24.7 24.7 24.7 24.4 25.1 25.0 24.6 24.6 24.4 S...
Q In Q11 208 8I4 818 R8I3 DO (mgIL) 8.21
- 7. J
.9 80 8.3 8.1 8.210
- 8. 2 8.31 8.2 8.3 Conductivity (jumhoslcm) 212 213 213 w
216
~
203 206
~
2111 Temnerature ("Cl pH (SU) i DO (mg/L)
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^X. I
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<0.1 Temperature (0C)
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Tpmnnrahure N.1 Tempratue CC
_I 8.15 8.14 8.35 7.991 8.267
.8 81
.0 81
.5 82
.6 85
.2 8.1 8.01 8.4 8.31 8.8 8.38 8.4
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S-0"1-MZ.
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Environmental Testing Solutions, LLC Page.
01 8 Species: Ceriodaphnia dubia Client:
-T-JA"
- rh-
%AlA Full-strength Chemistry:
Date: O S O OX-VjAu-Ilo Parameter Sample Number:
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1 2
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Species: Ceriodaphnia dubia Client:
"F
- P1
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Date: 6d-01-O-L-Concentration Parameter 0
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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/0 0 2, Methods 1000.0)
Species: Pimephales promelas Summary of Chemical Analyses Client:
Sequoyah Nuclear Plant UV-Treated Teat date:
May 07-14, 2002 Project number:
324 Reviewed by:
Pimephalespromelas 24.31 25.01 24.61
J.Jl 24.2 24.8 24.5 0.2 Overall Temperature (C)
(including all concentrations for initial and final temperatures) 24.2 25.3 24.5 0.2
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephales promelas Daily Chemical Analyses Client:
Sequoyah Nuclear Plant UV-Treated Test dates:
May 07-14, 2002 Project number:
324 Reviewed by:
Concentration Parameter Day 0 Day 1 Day 2 Day 3 Day 4 D
Day 6 Inta ia nta ia nFinal ina Innia Fina Itil Fia Initial Final initial Final 7.72 3,2 Day.
8.Day pH (SU) 8.27 8.12 8.22
- 7.
8.24 8.00 8.25 7.90 8.22 7.85 8.18 23 7.4 DO (mgfL) 7.9 7.6 8.1 6.8 8.2 7.8 8.1 7.1 7.9 6.9 7.9 7.5 7.9 Conductivity (pmuhos/cm) 315 296 310 03 Control Aalinity (mg/L CaCO3) 63 64 65 Hardness (mg/L CaCO3) 8 89 82 Temperature (C) 24.5 24.3 24.6 24.5 24.4 24.6 24.7 24.3 24.6 24.8 24.3 24.5 24.3 24.5 pH (SUJ) 8.2+8 8.12 8:.ý22 7.0 8.23 8.2 8.241 7.94 8.21 7.8 8.17 7.6 8.211 7.481 DO (mgfL) 7.9 7.71
- 8.
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Conductivity (Rmhos/cm) 300 299 289 287 288 289 Temperature CC) 24.6 24.3 246 24.5 24.5 24.7 24.2 24.8 24.8 24.4 24.5 24.3 24.5 pH (SU) 8.25 8.10 8.21 7.76 8.20 8.1 8.16 7.92 8.20 7.45 DO (mg/L) 7.9 7.7 8.0 7.0 8.0 8.11 7.31 7.91 6.8 777.4 7.8 6.5 22%
Conductivity (pmhos/cm) 283 279 280 277 270 274 274 Temperature (*C) 24.7 24.3 24.5 24.5 24.7 4.6 24.7 24.2 25.0 24.8 24.4 24.5 2.5 pH (SU)"
8.24 8.10 8.20 7.70 8.161 7.94 8.20 7.93 8.15 7.72812 7.96 8.1 743 DO (mgfL) 7.9 7.7
.0 6.9 8.1 7.6 8.2 7.4 7.9 6.9 7.4 43.9%
Conductvty (pmhos/cm) 251 48 253 249 240 Temperature CC) 24.3 24.5 24.3 24.6 24.6 24.6 24.2 25.1 4.5 pH (SU.)
8.20 8.08 8.18 7.791 8.121 7.941 8.17 7.80 8.12 7.84 8.081 7.961 8.141 7.421 DO (m*gL) 8.0 7.7 8.1 7.5 8.1 7.0 7
7.3 72%
Conductity (pimhos/cm) 211 214 210 20 Temperature (CC) 24.7 24.3 24.5 24.4 24.7 24.6 246 24.2 2
pH (SU) 8.17 8.08 8.15 7.71 8.06 7
DO (mg/L) 7.9 8.0 Conductivity (jmhos/cm) 169 164 100%
Alkalinity (mg/L CaCO3) 57 595 Hardness (mg/L CaCO3) 7 76 Total Residual Chlorine (mg/L)
<0.
<0 Temperature CC) 24.81 24.3 24.5 24.3 24.8 246 24.6 24 25.3 24.8 24.4 24.5 24.2 24.5 pH (SU) 8.14 8.10 8.13 7.74 8.07 7.94 8.14 7.87 8.08 7.76 8.02 7.9 8.12 DO (mg/L)
- 7.
7.7 8.0
- 7.
8.0 7.6 8.1 7.1 8.0 6.8 8.0 7.4 7.9 Conductivity (pinhos/cm) 167 169 172 168 165 168 167 100% Intake Alkalinity (mgfL CaCO3) 19 Hardness (mg/L CaCO3) 72 Total Residual Chlorine (mg/L)
<0.10
<0.10 "Temperature 24.5 24.3 24.7 24.6 24.7 24.2 25.0 24.8 24.4 24.5 24.3 24.5
Species: Pimephales promelas Client: --
'A- &`7_ -I A A Full-strength Chemistry: 6 0LL.
10 Parameter Sample Number:
Control Batch:
1 2
3 oC-06-07
-.oq-o2. o"-iA.ca pH (S.U.)
2.-
- .2' DO 7.0 (mg/L) d.0 1.01 Conductivity 16qI 315 7-4, (o (pmhos/cm)
Alkalinity (mg CaCO3/L) 51 64 59 o*
aS Hardness (ma CaCOJL) 716 12_
Chlorine 40,10
-<0,10
- 0. 1o Collection start date O*O-Og-2 O*-O7&.oZ oS-*-oZ Collection end time J0 M
MR Grab or Composite 73 J&ot"
- o~i-(duration)
LM49Oi Te CVt1rT-CoieM' Temperature (°C) 0 V upon receipt 0__,____.
Physical P.ALe -RM9 Ouet characteristics c
-TA-4 C-LeM.
Cj-j'h Dates sample used cco-OZ 0-Oq--z, as-&6
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- z.
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ETS Project and 505(
Sample numbers ozowO7. 01 o205Oq.0 oZOI-.
0 Date:
0S6-O'-O2.
MAL
Species: Pimephales promelas Client:
Full-strength Chemistry: I NhAKe Parameter ieNmbr 1
2 3
pH.
9.l.0"1 C', o DO
-9q
.0 Conductivity 1"
16e umos/cm)
Alkalinity O t.::r Hardness
-7 I'/
-1 (
in aC__-
Chlorine 4 0110 xOJO 10 I40 Collection start date OZ-- c*--oioCZ Collection -end time 11[c 41 I 12s Grab or Composit---e*
. o_*3**
(duraon
°Cce~e 4f Temperature CC)
- 0. 34c,
- 0. a-3C IX C upon receiRL tt M
olt Physical fA/e "/W /qe 1' t
characteristics C-.M.
C.&-
-rA.
Dates sample used o0-'-
o-L 0qoý2 osI -C" 054O-02.
¢-
0"o 9-12~q-a" ETS Project and 32-*oq 6251 0ows1 (.,a Sampe numbers zoTo Date:
0-'0
- 2.
Control Batch:
~-O ~
-119
-7.9O argO 315
?9(0 p503 Jq'j S9 OW! 9
Species: Pimephales promelas Client:
TW/
a.-TfA~~
Date:
o*-01-0Z Li
Page 8 ot 8 B T'!
1-f C' I*nviroflmeltaI iesting 301ULioiIs, Species: Pimephales promelas Client: "r4A-SeLWAA
_ 4aI Date: 650-7"-02---
Da Concentration Parameter 3
I5e j6 CONTROL pH (S.U.)"*
"*'.*O S
- .00
- ,Z-3".
DO mg)A Conductivity
_._o3_
CL,030
-I" wA ~
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( os/cmn Temperature
- q.
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- q.
"DO (mgIL)
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I
Environmental Testing Solutions, LLC Page Page I
of__J__
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Analyst [Z I-I Date analyzed 6fj-1 Iodide reagent:
I IQ AlO V Acid reagent:
0 p0q4 Slope: L Calibration:
Refernce tun ar num er L
e levels of th amls Note: For samples with a residual chlorine of > 1.0 mg1L. the calibration range must be adjusted to bracket the chlo0rinelvsofteame.
characteristics Residual chlorine I%RPD = {(S - D) IL(S+JJ)LLII X J`l (M
(acceptable range -+/-~ 101%)
D4<0.00L40' 0
Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Reviewed by Date reviewed I
Environmental Testing Solutions, LLC Page _____
Page
(
ofJ1 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pHI/lon Meter Analyst Date analyzed Iodide reagent:
Acid reagent:
Slope:
Calibration:
0.10u gl 1.00 m/
Reference standard number j.A.
_3J.I Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
I Reviewed by ' [--1--2 Date reviewed O
I U
0-4
_CK-1 -OZ
Environmental Testing Solutions, LLC Page ___
Page I_. of I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Analyst I II-II1 Date analyzed C6-I1 0_-
Iodide reagent:
Acid reagent:
Slope:
D 0. ýo-2 I 6%)
1
- were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Reviewed by Date reviewed
Page jLOf Environmental Testing Solutions, LLC Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [ji 7i et 4
Date analyzed [~~oJ Titrate samples to pH = 4.50 S.U.
Tit rann and multipfer determination:
PH of Normeality 'B~in DeIonized Titrant check Be water reference standard MI n
4.5 S.U.
number number Reviewed by:
=
(a rP Date reviewed:
6-Oo-
Environmental Testing Solutions, LLC Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [I& II Date analyzed 0[.gZ.o 2 Titrate samples to pH = 4.50 S.U.
0and multipier determination:
pH of II Normality (N) of HzSO4 pH Factor or Multiplier Deionized Titrant check dIlgin--
Total
= (5 ml Na2CO3 x 0.05)/E
= (N x 50000)1 100 ml sample water reference standard ml ml
=-- 0.25/E N x 500
= 4.5 S.U.
number number
__l (accep) e0.018 - 0.022)
Laboratory control standard:
Reference True value Sample Alkalinity (MV)
% RS = MV / TV x 100 I standard number (TV) volume Begin End Total Multiplier (mg CaCO3/L)
(acceptable range (mg CaCO03 L)
(ml) ml ml ml 90 to 110%)
1,46sstq 1o 0
olo I I00 wo o
1, ii e
9 e Duplicate sample precision:
Sample Alkalinity
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L)
I(S - D) JI(S+D)12I) x 100 number (ml) ml ml ml L (acceptable range -:6 10%)
So I. L O -* z ft 14 C to o I q t " *5 "
6 (0 -4 s 0,
Duplicate-0 D
OS-14-07-O 1__
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. Z55.
1.o
ý q(*
T D
to"/
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (ing CaCO3/L)
"(mg CaCO3/L)
(ml) ml ml ml 5U iou Z5.6 I i1.q
.6 1 lt Sample alkalinity (B)
Measured spike value (MV)
% R = MV I SV x 100 (ag CaCO3/L)
MV = A - B (acceptable range (mg CaCO-VL) 75 to 125%)
(a*
qb LA Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC0/L) o S -0.2 Ssv W z kj*%.9 3.z 4.o.
_3_Z_
_1 06-o.o 3-.
.6 Z
Z of5o-?
C3 QI rojz Qfl. 01,"
5' Z(.*
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I Z*
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Z*,o 5j.*
020SII.O....
-*3 3..0T..
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_ALC.A r-P 3q._,
_'Z.e Z_
Reviewedby:
I Date reviewed: [OS120-0 Z I
a
Page
-fiof Environmental Testing Solutions, LLC Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst [
C~
Date analyzed 06-L1).OZ Tirant no Tma and mut-ipl-er d mination:
Titrant Normality check Begin End Total Normality (N) of EDTA reference standard ml ml ml
=
O.21E number number (acceptable range -0.018 - 0.022) tReference True value Sample Hardness Dtanar number (T) volume Begin End Total Multiplier (Mg Cac (Mg CaCO/L) ml(
ml ml ml 40 50 C
I j
-Z0.0 SCI vavg Samle measurements.*
Sample volume Begin End Total Sample number Sample ID ml ml nl ml Multipler Blank 6
0 0
...0
?_o.L,
'__ t) shouldbe=Om CaCO3IL
- 0.
t u3~~~
Z 8,
ao
.3 C6.0 3,..
4-:L4,5 31.!5 L130.3
- %z~~z 45.__6 41 4.1_______
.Z
'4e~
I LAfl N: oaiof
- oaiu, sampleme._tde*iwb 5
Datear-Note: If >I 5ml oftittrant is used, sample must be diluted.
Reviewed by: E:J]
Date reviewe Hardness
_ 93D 89O d
ZEnvironmental Testing Solutions, LLC Page 2-of Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [
o.*7--Z Date analyzed
.i and muplti er determination:
Titrant Normality c e in End reference standard ml number number q-4
"-46.
"-,L4 Date reviewed [II-i 70-0I.
]
Note: If > 15ml of titrant is used, sample must be diluted.
Reviewed by:
C/I \\I
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water Test number 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 Test date 7-day IC25 (g KCI/L) 06-13-00 09-19-00 10-24-00 11-07-00 03-13-01 06-26-01 07-17-01 08-21-01 09-25-01 11-01-01 11-06-01 11-27-01 12-12-01 01-04-02 02-05-02 02-13-02 02-26-02 03-28-02 04-23-02 05-07-02 0.70 0.66 0.77 0.65 0.69 0.53 0.74 0.62 0.60 0.71 0.66 0.57 0.63 0.49 0.61 0.65 0.56 0.70 0.61 0.54 Note:
7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean IC25).
S Standard deviation of the IC25 values.
S
= Standard deviation corresponding to the the 75th percentile CV.
8A.75 = 0.38, as determined by USEPA for the method and endpoint.
SA.9O = Standard deviation corresponding to the the 9 0 th percentile CV.
SA.90 = 0.45, as determined by the USEPA for the method ana enupu--L.
CV = Coefficient of variation of the IC25 values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-00 3. US Environmental Protection Agency, Cincinnati, OH.
Organisms obtained from Aquatic BioSystems, Inc.
05-07-02.xls CT (g/L KCI) 0.68 0.71 0.69 0.69 0.67 0.68 0.67 0.66 0.67 0.67 0.66 0.66 0.64 0.64 0.64 0.64 0.64 0.64 0.64 S
0.03 0.06 0.05 0.05 0.08 0.08 0.07 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.07 0.07 0.07 0.07 0.07 SA.75 0.26 0.27 0.26 0.26 0.25 0.26 0.25 0.25 0.25 0.25 0.25 0.25 0.24 0.24 0.24 0.24 0.24 0.24 0.24 SA.90 0.31 0.32 0.31 0.31 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.30 0.29 0.29 0.29 0.29 0.29
.0.29 0.29 CV 0.05
'0.08 0.08 0.07 0.12 0.11 0.11 0.11 0.11 0.10 0.10 0.10 0.12 0.12 0.11 0.12 0.11 0.11 0.11
- ÷
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1
4
?
% N1 Iv
'I Oi?
0
0g 0
1 1
1
'
Test date 0
7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
......... Control Limits (mean IC25 +/- 2 Standard Deviations) 1.2 1.0 0.8 0.6 0.4 0.2 I
I I
I I
I I
I I
I I
I I
I I
I I
Control Limits Set According to +/- 2 Standard Deviations.
.°
°..................
S
.... I.........................
I I
I t
I I
I I
I I
I I
I I
I I
I I
I I
9=ý U
U 00
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 2.0 I
I i
I I
I Warning and Control Limits Set According to 75th and 90'h Percentile CVs.
1.5 1.0 S0.5 0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.11) is less than the 7 5th and 90 d percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
-Warning Limits (mean IC25 +/- SA.75, SA.75 = 0.38, as determined by USEPA for the method and endpoint)
Control Limits (mean IC25 +/- SA.90, SA.90 = 0.45, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephales promelas using Moderately Hard Synthetic Water Control Test number Test date Survival (O/)
1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 06-13-00 09-19-00 10-24-00 11-07-00 03-13-01 06-26-01 07-17-01 08-21-01 09-25-01 11-01-01 11-06-01 11-27-01 12-12-01 01-04-02 02-05-02 02-13-02 02-26-02 03-28-02 04-23-02 05-07-02 100 100 97.5 97.5 92.5 100 100 100 100 100 100 97.5 95 95 97.5 100 100 100 100 97.5 Control Mean Growth (mg/larvae) 0.58 0.67 0.83 0.67 0.34 0.63 0.52 0.89 0.85 0.54 1.00 0.70 0.82 0.80 0.95 0.65 0.64 0.63 0.71 0.46 CV CT for Control
(%)
Growth CV (o) 13.2 5.3 13.3 10.5 6.5 9.8 9.1 8.3 4.9 2.5 7.4 22.8 16.6 10.1 6.9 8.0 3.3 11.2 12.8 12.3 9.2 10.6 10.6 9.8 9.8 9.7 9.5 9.0 8.3 8.3 9.5 10.0 10.0 9.8 9.7 9.3 9.4 9.6 9.7 MSD PMSD CT
(%)
for PMSD (%)
0.12 0.07 0.22 0.13 0.08 0.19 0.07 0.13 0.09 0.10 0.17 0.18 0.19 0.18 0.21 0.10 0.11 0.13 0.19 0.07 20.0 11.2 26.9 19.3 22.8 30.6 13.8 15.1 10.4 18.6 16.9 26.1 23.5 22.0 22.2 15.5 16.5 21.4 26.2 15.4 15.6 19.4 19.3 20.0 21.8 20.7 20.0 18.9 18.9 18.7 19.3 19.6 19.8 20.0 19.7 19.5 19.6 20.0 19.7 Note:
CV = Coefficient of variation for control survival.
On average, the CV for control growth is 9.7% in Environmental Testing Solutions, LLC Pimephales chronic toxicity tests.
Lower CV bound determined by USEPA (10" percentile) = 3.5%.
Upper CV bound determined by USEPA (901P percentile) 20%
MSD Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control.
Lower PMSD bound determined by USEPA (10"h percentile) = 9.4%.
Upper PMSD bound determined by USEPA (90"h percentile) 35%.
CT = Central Tendancy (mean Control Growth CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
05-07-02.xls I.
I 1.-
4--
r"I
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium 40 30
~20 10 0
Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
S-
-Central Tendency (mean PMSD)
.........Lower and Upper PMSD Bounds Lower PMSD Bound (101h percentile) = 9.4%, Upper PMSD Bound (90 te percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Page 01or5 Ys~.
T T r
Daily feeding and renewal information:
Morning feeding Afternoon feeding Morning feeding Afternoon feeding time time l(000 1014 16o 0145s.
1606 001b Ito
)oooJ51 Test initiation, renewal, or termination time sIzo Comments:
Dilution preparation information:
KCI CHM number:
rul H 0J1 Stock preparation:
50 g KCI/L: Dissolve 50 g KCI in I-L Deionized water Dilution prep (mg/L) 300 450 600 750 900 Stock volume (mL) 6 9
12 15 18 Diluent volume (mL) 994 991 988 985 982 Day 0
1 2
3 4
5 6
7 Analyst Analyst
/c AL/
K, Date 0g-C7-07.
-CC08-O'L i C-S-10oL 05.10-0-OL j%, tS -6"2 Iomns 1
ironmental leslig Solutionsu, Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promelas PpKCICR Test Number: 18 w w'eAIVN
Page 3 of 5 vironmental Testing Solutions, LLC Calculations and data reviewed:
I PpK C1C R T est N um ber:
I _ _
I4owwwwry b
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses PpKCICR # 18 May 7.14, 2002 Dunnett's MSD value:
PMSD:
0.0707 15.4 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision-The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control (determined Lower PMSD bound determined by USEPA (10th percentile) = 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
05-07-02.xis Organisms obtained from Aquatic BioSystems, Inc.
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date 517/02 Test aD PpKCICR Sample 1D0 REF-Ref Toxicant End Datat 5/14/02 Lab 1D:
ETS-Env. Testing Solutions Sample Type KCL-Potamsiu= chloride Sample Dam Protocol:
EPAF 91-EPA Freshwater Test Species:
PP-Pimephales promelas Cooe-mg/L 1
2 3
4 D-Conml 1.0000 0.9000 1.0000 1.0000 300 1.0000 1.0000 0.9000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.80 0.7000 1.0000 0.8000 750 0.8M0 0.9000 0.8000 0.8000 900 02000 0.3000 0.4000 0.1000 Transeform: Arcsin Square Root Rank 1-Tailed Number Total Conc-mg/L Mean N-Mean Mean Mm Max CV%
N Sum Critical Resp Number D-Control 0.9750 1.00 1.3713 1.2490 1.4120 5.942 4
I 40 300 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4
18.00 10.00 1
40 450 1.0000 1.0256 1.4120 1.4120 1.4120 0.000 4
20.00 10.00 0
40 600 0.8250 0.8462 1.1544 0.9912 1.4120 15.615 4
12.50 10.00 7
40 750 0.8250 0.1462 1.1426 1.1071 1.2490 6.209 4
10.50 10.00 7
40
- 900 0.2500 0.2564 0.5124 0.3218 0.6847 30.428 4
10.00 10.00 30 40 Amillacy Tet Statisc Critical Skew Kurt Shapiro-Wllh s Teat indicates rotmal distribution (p>0.0I) 0.948615253 0.114 0.427279336 1.241498999 Equality otvnmmrcau-eot be confirmed Hypothesis Test (1-tal, 0.05)
NOEC LOEC ChV TU Stee's Many-Ooe Rank Test 750 900 821.5838363 Maximum Llkelllood-Probit Parameter Value SE 95% Flduclal Limits Control Chi-Sq Critical p-value Mu Sigma lter Slope 24.83109115 6.391501454 12.30374806 37.35843424 0.025 2.535851211 7.814724922 0.47 2.930516378 0.040272093 22 Intercept
-67.76791931 18.77373555
-104.5644417 -30.97139691 TSCR.
0.099690478 0.023695382 0.053247527 0.14613342a 1.0
/
Peint Probits l sg/L 95% Fiducial LimIls 0.9 ECO1 2.674 686.7987265 537.058375 747.6565068 "EC05 3.355 731.6016577 600.8859727 781.3018378 0.8 ECI0 3.718 756.6694322 650.5914712 800.3850597 0.7 ECI5 3.964 774.064301 680.0050895 813.9142816 EC20 4.158 781.1747746 704.0221999 825.1876413 0
EC25 4.326 800.4151229 724.9761854 835.3575863 0.5 EC40 4.747 832.3645221 778.0726786 864.3273455 5 04 ECS0 5.000 852.150649 808.6100921 885.7921691 EC60 5.253 872.4071154 836.1133631 912.3091936 0.3 EC75 5.674 907.1508087 873.6396115 969.6457235 0.2 ECSO 5.842 921.319425 886.269403 996.4079068 0.1 EC85 6.036 938.1142293 900.0531996 1029.871372 EC90 6.282 959.6815291 916.5147209 1074.967135 0.0 EC95 6.645 992.5629769 940.0032492 1147.249305 10 100 1000 10000 EC99 7.326 1057.312313 983.3092802 1299.356351 Dose mg/
Dose-Response Plot 0.0
-0.8
- 0.
0.5
,-0.3 0.2 0A o I*
n'Z n7".Al" ýlc
Environmental Testing Solutions, LLC Statistical Analyses Larvall Fish Growth and Survival Test-7 Day Grow Sltat Dot=
537/02 Test ID:
PpKCICR Sample ID:
REF-Ref Toxicat End D=s 5114/02 Lab a.
ETS-Env. Testing Solutions Sample Type:
KCL.PotasiWm chloride Sample Da=e Protocol:
EPAF 91-EPA Freshwater Test Species:
PP-pimephales prmelas Coumenas:
Co-esmg/L 1
2 3
4 DContol 0.4440 0.3872 0.5186 0.4822 300 0.4208 0.4538 0.4311 0.4935 450 0-3496 0.4177 0.3787 0.4287 600 03425 0.2740 0.2925 0.3473 750 0.2579 0.3274 0.2673 0.3636 900 0.0630 0.0822 0.1126 0.0390 Transform: Untransformed I-Tiled Isotonic con il Mean N-Mesn Mean Niln Max CV%
N t-Stat CriUeal WSD Mean N-Mean D.Control 0.4580 1.0000 0.4580 0.3872 0.5186 12.265 4
0.4500 1.0L00 300 0.4498 0.9821 0.4498 0.4200 0.4935 7.165 4
0.279 2.410 0.0707 0.4490 0.9821 450 0.3937 0.0596 0.3937 0.3496 0.4287 9.241 4
2.192 2.410 0.0707 0.3937 0.8596 "600 0.3141 0.6850 0.3141 0.2740 0.3473 11.602 4
4.904 2.410 0.0707 03141 06.38
- 750 0.3041 0.6639 0.3041 0.2579 0.3636 16.523 4
5.245 2.410 0.0707 0.3041 0.6639 "900 0.0742 0.1620 0.0742 0.0390 0.1126 41.924 4
13.076 2.410 0.0707 0.0742 0.1620 Asuy T Stastsc Critcal Skew Kurt ShApim.-ila s Test iseg -W tisibodon (p > 0.01) 0.956860721 0.084 0.025996871
-1.030953136 Bha oilee s Test indicates ep-A e vatiances (p - 0.90) 1.646460414 15.08631706 Hypothesis Test (1-t4l, &.05)
NOEC LOEC ChV TV WDU MSDp MSB WE F-Prob O
Dunnetfs Tea 450 600 519.6152423 0.070734453 0.154442041 0.000935563 0.001022691 1.1E-09 3,10 Linear Interpolation (80 Remmples)
Point mg/L SD 95% CL(KEp)
Skew IC05 339.29 88.10 0.00 449.63
-0.8574 ICIO 400.49 63.04 140.29 517.97
.0.7585 IC15 458.24 47.77 277.09 543.09
-0.9195 1.0 IC20 501.40 38.76 363.05 585.41
-0.5792 0.9 IC25 544.55 41.44 438.65 635.04 1.2997 0
IC40 769.09 26.54 635.98 795.77
-2.208l 0.0 7990 1 1.39 7
33.26 024.51
.0.2200 0.7 w 0.6 0.5 0.4 0.3 0.2 0.1 0.0[2 0
200 400 600 800 1000 Dose mg/L Dose-Rtesponse Plot 0.6 0.5 04--------------------------------------------------------------
1-tail.
0.05 level
......of significance a 0.3
- 0.2 0.1 0 o S A-A A-..I
,ironmental Testing Solutions, LLC PpKCICR Test Number:
I&
f,)ONMURA r' etb MHS Control Chemistry:
Parameter Control Batch:
osN-e o-05-0-0 s*
pH PHu,
g 2 -
- a.
R. IZIS (S.U.)__
DO (mg/L)
Conductivity (gmhos/cm) 3Z 3tlI Alkalinity (mg CaC0 3/L) 6`5 V4____
Hardness r(mg CaCO3/L)
Acceptance Criteria for Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 300 mg KCI/L 840 - 890 450 mg KCI/L 1100-1180 600 mg KCIJL 1390- 1460 750 mg KCI/L 1660-1710 900 mg KCIlL 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCI1/L)
Page D 01 pvironmental Testing Solutions, LLC PpKCICR Test Number:
elvironmental Testing Solutions, LLC-
/.
150mg KCl/L OH (S.U.)
DO (mg/L)
I i Conductivity (uhS/CM) 1 1j01 Temperature COC) 2-4. (o I zq. 1 1
11 IqB1 I fa.za
-+II A
II I
Ii I
ii
.1 2 M. tz~
.qs pH (S.U.)
.Z
-4
)00 ing KCI/L Conductivity (gmnhos/cm)
/
I Temperature Initial Ii Final I initial I Final I Initial II Final 1 Initial H Final
'54tckL PpKC1CR Test Number:
I k I
ceatration Parameter 345Z NTROL pH (S.U.)
7I~
8-26 f?
DO (mg/L~)
60S Conductivity 3~
(limhos/cm)Iq a
Temperature A-2q1
'..*qS zq.3 Z.q, pH (S.U.)
A.LD1.ZZ DO (mg/L) o.)
8 mg KCU/L ConductivityM 85 Temperature 2,
L-zz3qz~
0 q(
1 -I
.4 IMCt 24.4 z q DO (mg/L)
- 1.
uig KCIfL Conductivity (gmnhos/cm)
I]qz IN 1
Temperature 246 4-Z z4.1T 2'14 zzPI 4
q.c PH (S.U.)
8-1 4
G N3 e,
.4823 mg KCIIL DO (m&UL (0.
Conductivity Ounlios/cm) jLJOZ Temperature 1-9461 z'4.?
zq4q L2-11 z
24.q A.7-(.0 9 -4.81 1 12 1.,
1 4AS 1
I 2q.7-I z 4, ST-1 7-ý- 1 1
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test number 1
2 3
4 5
6 7
8 9
10 11 12 13 14 15 16 17 18 19 20 Test date 7-d IC25 (g/L NaCI) 12-17-00 12-19-00 12-19-00 01-09-01 02-06-01 03-07-01 04-04-01 05-09-01 06-06-01 07-10-01 08-08-01 09-12-01 10-10-01 11-01-01 12-05-01 01-09-02 02-05-02 03-19-02 04-09-02 05-07-02 0.89 1.01 0.97 0.95 1.11 1.04 1.02 1.10 1.07 1.05 1.02 1.02 1.03 1.00 1.05 0.91 1.07 1.03 1.03 1.05 Note:
7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
SA.75 = Standard deviation corresponding to the the 75th percentile CV.
SA.75 = 0.45, as determined by USEPA for the method and endpoint.
SA.90= Standard deviation corresponding to the the 9 0 1h percentile CV.
SA.90 = 0.62, as determined by the USEPA for the method and endpoint.
CV Coefficient of variation of the IC25 values.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
05-07-02.xls CT (g/L NaCI) 0.95 0.96 0.95 0.99 0.99 1.00 1.01 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.02 1.02 S
0.08 0.06 0.05 0.08 0.08 0.07 0.07 0.07 0.07 0.07 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06 SA.75 0.43 0.43 0.43 0.44 0.45 0.45 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 0.46 SA.90 0.59 0.59 0.59 0.61 0.62 0.62 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 0.63 Cv 0.09 0.06 0.05 0.08 0.08 0.07 0.07 0.07 0.07 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06 0.06
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 0.50 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
..........Control Limits (mean IC25 +/- 2 Standard Deviations) 1.50 1.25 1.00 0.75
- 0
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water I
I I
I I
I I
I I
I I
I 1
I I
I Warning and Control Limits Set According to 75th and 90eh Percentile CVs.
Since the coefficient of variation for the set of 7-d IC25 values (0.06) is less than the 75"' and 90"' percentiles determined by the USEPA. these percentiles were used in setting the warning and control limits.
I I
I I
I I
I I
I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 '-+/- SA.75, SA.7S = 0.45, as determined by USEPA for the method and endpoint)
Control Limits (mean IC25 +/- S A.90 SA.90 = 0.62, as determined by USEPA for the method and endpoint) 2.5 2.0 Q
z IA t
1.5 1.0 0.5 0.0
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Watei Test TControl Control Mean number Survival Reproduction
(%)
(offspring/female) 1 2
3 4
5 6
7 8
9 10 11 12 13 14 15 16 17 18 19 20 12-17-00 12-19-00 12-19-00 01-09-01 02-06-01 03-07-01 04-04-01 05-09-01 06-06-01 07-10-01 08-08-01 09-12-01 10-10-01 11-01-01 12-05-01 01-09-02 02-05-02 03-19-02 04-09-02 05-07-02 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 25.6 24.8 25.1 28.0 22.1 24.0 25.0 27.9 27.8 25.0 30.9 28.6 24.9 27.0 24.8 27.5 23.1 26.3 26.2 27.3 CV CT MSD PMSD for Control
(%)
Reproduction CV (%)
4.6 9.5 19.2 8.4 10.1 8.3 10.0 8.9 5.8 9.4 2.4 6.6 8.8 6.5 9.1 7.5 10.1 8.6 12.3 8.1 7.1 11.1 10.4 10.4 10.0 10.0 9.9 9.4 9.4 8.8 8.6 8.6 8.5 8.5 8.4 8.5 8.5 8.7 8.7 3.3 4.6 5.0 3.5 3.1 2.4 2.9 3.1 3.5 2.8 2.7 2.7 2.5 3.1 4.8 4.5 2.6 3.0 2.8 2.3
(%)
for PMSD (%)
12.9 18.4 19.8 12.4 14.2 10.1 11.8 11.0 12.5 11.2 8.7 9.4 9.9 11.6 19.5 16.3 11.1 11.2 10.7 8.4 15.6 17.0 15.9 15.5 14.6 14.2 13.8 13.7 13.4 13.0 12.7 12.5 12.4 12.9 13.1 13.0 12.9 12.8 12.6 Note:
CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 8.7% in Environmental Testing Solutions, LLC Lower CV bound determined by USEPA (10'h percentile) = 8.9%,.
Upper CV bound determined by USEPA (90th percentile) = 42%
UMSD
= Minimum Significant Diference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 12.6% from the control.
Lower PMSD bound determined by USEPA (10h percentile) = 11%.
Upper PMSD bound determined by USEPA (90"' percentile) 37%.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
05-07-02.xis
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
S-
-Central Tendency (mean PMSD)
Lower and Upper PMSD Bounds Lower PMSD Bound (10"' percentile)= 11%, Upper PMSD Bound (90"' percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
50 45 40 35 30 25 20 15 10 5
0
Page 1 otr7
mnnfal Tpgtinu Snititions. LLC J+/-iII VII UAIhIIt,*1t441
,.J'.*fl Sodium Chloride. Chronic Reference 'oxicant T'est (EPAI600/4-91/002 Method 1002.0)
Species: Ceriodaphnia dubia Date Time Aalsyst CdNaC Teststart OS-L"6?0" n
12 4X \\
Test end 6s.t4 -
Dilution preparation information:
NaC1 CHM number:
CA*&
Stock preparation:
100 g NaC1/1 (dissolve 50 gNaCl in 500 ml deionized wtr Dilution prep (mg/L) 600 800 1000 1200 1400 Stock volume (mL) 9 12 15 18 21 A1 IA8.8 1485 1482 1479
-1ICR rest Number:
Comn umts:
LDlluent VOIUme Ikmlh)
I
'-'1
,l.I Test organism information:
Tt rmatin:
Organism age:
c 4z-q Randomizing template:
-'ýOe4.
Date and times organisms cS'-
OL.0(-.-z 10 \\-rc tcO7--
Incubator number:
2 were born between:
Organism source:
O' 0- o0 P-- --
YCT batch:
q4
, 4,0O 2 Transfer bowl information: [pH = C. 2-5 Temperature (°C)=t4.,
Selenastrum batch: AS aO -1,4.2, CONTROL Survival and Reproduction Data Replicate number D ay 1
23 4
6 8
9...
-," - - -]
10 1
Young produced (N
t 0
- 0)
C 0
(
Adult mortality
\\..
L V
L.-
(4-z
=puced Yon prod 0*
)IOIo___I©_O_
Adult mortality S
Young produced 0~j 0
~
L Adult mortality 4
Y oung produced I l
o 0
j7i+
Adult mortality
_I_'
5 Young produced8 Adult mortality I
L.
I-Young produced i l 0
0 l
y Ic o 4 i1j Total young produced
- 2)
- Q j
Zt Z9 C) 2o Final Adult Mortal1 X for 3r, Broods
-<n
-htn reviewed:
Test Renewal, Feeding, and Radomization Record Label Mon Tue Wed Thu Fri Sat Sun 1 Date "A
O5S-alff1 0 -5
()3-&
()"
Time
!r lza 1/51 im I i1 MV I~
U51 Analyst Shelofat ILocation Control information:
% of Male Adults:
% Adults having 3' Broods:
% Mortality:
Mean Offspring/Female:
%NCV:
II5II
160%
Acceptance criteria Summaryo test en ypoints (mg NaC1/L):
_20%
7-day LCS0
_q 0_
>80%
NOEC
- 20%
LOEC
/000
_ 15.0 offspring/female" ChV oqq.
< 40.0 %
IC25 0qq. 2-JJiLVl a~llL U
lxxxx i AtO.
tzA 9v.
Comments:
I I
page 2 of 7 Environmental Testing Solutions, LLC CdNaCICR Test Number:
Concentration:
i
% Mortality:._
2 M~ean Off'print1/Fiemale:
% Rduc:ti om Control:
Calculations and data reviewed:
Concentration:
% Mortality:
Mean Offspringeemale:
2
% Reduction from Control:
7 Calculations and data reviewed:
I
Page :3 ot 7 CdNaCICR Test Number:
"."L-1AflfA m,~
Naoi'lr.
Survival and Reproduction Data S
Calculations and data reviewed:
Qnlii*inne I.T.r Raab at Replicate number Day 1
2 3
1 4
5 6
7 8
9 10 1
Young produced r---
Adult mortality 2
Young produced 0
an r
Adult mortaity tL
-1 L--
~
'- t
-1' 3
Young produced 0
1 4I0 zI 0
Adult-mortality L
I L -
L t L-4o Young produced A
1 2
D mortality L.--**
- k.
- k.
5 Young produced C)C tion
- 0 Adult mortalityM y
6 Young produced 7
[Q4 7
L[ ICo10 Adultmortality
[_
'-_=
L-L,.J tL 7
Youngproduced
-I 0
Q O I
IO Adutmrtaft L
.. U.
__U.
-I
-'I I u Total young produced 1
7 I
2I Z 1204
-25 2T-o_17Z wI 03 Final Adult Mortali*
t]*
i I
I
-1
-i
'1 Concentration:
% Mortality:
0-,
Mean Offspring/Female:
I3. 6
% Reduction from Control:
I
'B7.
Calculations and data reviewed: 4 1200 mg NaCII Survival and Reproduction Data
_____Replicate number___
Dy2 3
14~
5 16 718 9
10 1
Young produced [91~~
Adult mortality
~
117~L.-
2 Young produced OF--
p01 0 Io 6
C) r~
Adult m ortality IL -
L.-..*
I I7-L--
- -I - --
L__
3 Young produced 0
2-
__110 C
Ada mortality -cz-t I~1
~I'-
U LI_
U 4
Young produced 0~
(o0
~
~
.LI~
C Adult mortality L-l -
-[U.~
I~
6 Young produced I__
Adult mortality A.-__
7 Young produced 0
1, I U IL T o t a l y o u p r d u e 51 0 1 z o 1 1 -
q1 FInal Adult Mortality T-.
T-L-W I~ k. -
K Concentration:
I%Mortality:G%
%Redutionfrom Control:
J~ilIIUIIU*
Ii
£*IV
+.
U~l Q
v o
I 1.f
JIRVIIRURJIiULaLLa l JL a-I I
Page 4 of 7 CdNaCICR Test Number:
-Z..Z-S,jrvjvaI and Renroduction i)ata number Day1 2-3 4
5
-6 7-8 9
10 I
Young produced Q
-)
j C) 00 Adult mortality R [ -
I L-2 Young produced jC)7
~
~ o~J I Adultmortality
\\-4_
I L--
I1L L
3 Young produced c-C C)
Fnl Adult Mor fitalt y.
t..
I,.
4 Young produced 0
0 Adul morrtalyity iea Younging/Fmalec Adult mortalityf__
onol _
6 uYoung produced I Adult mortality 7JYoung produced Q
Total young produced 1.0 33s
.1
'-41 1Final Adult Mortality Concentration:
[% Mortality:
Mean Ofsring/Femfale:
f
.1Reduction from Control:
&0 -6%
Calculations an(] data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
CdNaCICR #22 Test dates:
May 7-14, 2002 Revelwed by: /--
Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2
3 4
5 6
7 8
9 10
(%)
(offspring/female) variatn control Control 28 27 30 27 28 31 24 28 25 25 100 27.3 8.1 Not applicable 600 27 24 27 28 23 25 28 26 26 27 100 26.1 6.4 4.4 800 27 27 25 26 24 22 25 28 26 22 100 25.2 8.1 7.7 1000 24 24 22 24 25 27 23 23 21 23 100 23.6 7.0 13.6 1200 16 11 8
5 10 20 17 9
4 9
100 10.9 47.9 60.1 1400 2
8 5
8 3
3 8
5 7
4 90 5.3 43.6 80.6 Dunnett's MSD value:
PMSD:
2.866 10.5 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 12.6%/ from the control.
Lower PMSD bound determined by USEPA ( 10'b percentile) = 11%.
Upper PMSD bound determined by USEPA (90th percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
I _-/I
Environmental Testing Solutions, LLC Statistical Analyses Cerlodaphunla Survival and Reproduction Test-Reproduction Start Daec 5n1702 Test ID:
CdNaCICR Sample i1D:
REF-Ref Toxicant End Date:
5/14/02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
NACL.Sodium chloride Sample Date:
Protocol:
EPAF 91 -EPA Freshwater Test Species:
CD-Ceriodaphnia dubia Comments:
-Conc-mgIL 1
2 3
4 5
6 7
9 9
10 D-Control 28.000 27.000 30.000 27.000 28.000 31.000 24.000 28.000 25.000 25.000 600 27.000 24.000 27.000 28.000 23.000 25.000 28.000 26.000 26.000 27.000 800 27.000 27.000 25.000 26.000 24.000 22.000 25.000 20.000 26.000 22.000 1000 24.000 24.000 22.000 24.000 25.000 27.000 23.000 23.000 21.000 23.000 1200 16.000 11.000 8.000 5.000 l0.000 20.000 17.000 9.000 4.000 9.000 1400 2.000 8.000 5.000 8.000 3.000 3.000 8.000 5.000 7.000 4.000 Transform: Untransformed Rank I-Tailed Ilsotontk Conc,-mg/L Mean N-Mean Mean Min Mal CV%
N Sum Critical Mean N-Mean D-.Control 27.300 1.0000 27.300 24.000 31.000 8.108 10 27.300 1.0000 600 26.100 0.9560 26.100 23.000 28.000 6.373 10 88.50 75.00 26.100 0.9560 800 25.200 0.9231 25.200 22.000 28.000 8.111 10 80.00 75.00 25.200 0.9231
- 1000 23.600 0.8645 23.600 21.000 27.000 6.977 10 62.50 75.00 23.600 0.9645
-1200 10.900 0.3993 10.900 4.000 20.000 47.857 10 55.00 75.00 10.900 0.3993 "1400 5.300 0.1941 5.300 2.000 8.000 43.619 l0 55.00 75.00 5.300 0.1941 Auxliary Teats Statistic Critical Skew Kurt Kolmogorv D Test indicates normal distribution (p >0.0) 0.79925704 1.035 0.46940546 1.0849612 Bartletez Test indicates unequal variances (p = 1.11 E-03) 20.2808399 15.0863171 Hypothesis Teat (1-tlaO 0.05)
NOEC LOEC ChV TU Steels Many-One Rank Test 800 1000 894.427191 Linear Interpolation (80 Resamples)
Point mg/L SD 95% CL Skew IC05 636.666667 155.017213 358.664431 085.33333
-0.2340 ICIO 878.75 94.9980011 668.58167 1004.25561
-1.0865 ICIS 1006.22047 47.7651403 831.597222 1025.6365
-3.3118 1.0, IC20 1027.71654 11.7313368 1004.79496 1050.25581
-0.0532 0.9 IC25 1049.2126 12.28073 1025.37009 1076.86047 0.2916 1C40 1113.70079 17.0805645 1095.25067 1153.5577 0.8164 0.0 IC50 1156.69291 21.6175266 1131.84389 1207.59528 0.8727 0.7 I* 0.6 o0.5 0.4 0.3 0.2 0.1 0.0 0
500 1000 1500 Dose mg/L Dose-Response Plot 35
- 30.
25 20 115 10 5
0
Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation only.
Cerlodaphnla Survival and Reproduction Test-Reproduction Start Date:
5/7u02 Test ID:
CdNaCICR Sample ID:
REF-Ref Toxicant End Date:
5/14(02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
NACL-Sodiumn chloride Sample Date:
Protocol:
EPAF 9 1-EPA Freshwater Test Species:
CD..Cerodaphnia dubia Comments:
Conc-mg/L 1
2 3
4 5
6 7
8 9
10 D-Control 28.000 27.000 30.000 27.000 28.000 31.000 24.000 28.000 25.000 25.000 600 27.000 24.000 27.000 28.000 23.000 25.000 28.000 26.000 26.000 27.000 800 27.000 27.000 25.000 26.000 24.000 22.000 25.000 28.000 26.000 22.000 1000 24.000 24.000 22.000 24.000 25.000 27.000 23.000 23.000 21.000 23.000 1200 16.000 11.000 8.000 5.000 10.000 20.000 17.000 9.000 4.000 9.000 1400 2.000 8.000 5.000 8.000 3.000 3.000 8.000 5.000 7,000 4.000 Transform: Untransformed I-Tailed Coac-mg/L Mean N-Mean Mean MWn Mal CV%
N t-Stat Critical MSD D-Control 27.300 1.0000 27.300 24.000 31.000 8.108 10 600 26.100 0.9560 26.100 23000 28.000 6.373 10 0.958 2.287 2.866 800 25.200 0.9231 25.200 22.000 28.000 8.111 10 1.676 2.287 2.866
- 1000 23.600 0.8645 23.600 21.000 27.000 6.977 10 2.953 2.287 2.866 "1200 10.900 0.3993 10.900 4.000 20.000 47.857 10 13.087 2.287 2.866
- 1400 5.300 0.1941 5.300 2.000 8.000 43.619 10 17.556 2.287 2.866 Auxiliary Testa Statistic Critical Skew Kurt 0.79925704 1.035 0.469405458 1.884961203 Kolmogorov D Test indcate normal distributio n 1p, > 0.u 1, Bartetefa Test indicates unequal variances (p = 1.11E-03) 20.28083992 Hypothesis Teat (1-talt 0.05)
NOEC LOEC ChV TU MSDu M
Dunnetts Test 800 1000 894.427191 2.865523013 0.104 15.08631706 ISDp MSB MSE F-Prob df 4964213 857.9466667 7.851851852 5.6E-27 5,54 f'.
-* tt n,q IIII.?
znvir~lOntuentati-tl ebtilig 00OIU0lll 09IX_
CdNaC1CR Test Number: _7.
MHS Control Chemistiry:
Acceptance Criteria for Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the CONTROL 280-360 600 mg NaCI/L 1390 - 1590 800 mg NaCIIL 1780 - 1970 1000 mg NaClfL 2190 -2370 1200 mg NaCI/L 2550 - 2740 1400 mg NaCIJL 2910 - 3160 NaCI Stock 110500 - 116300
S. I o
/Il1cv 11 DSbo I
CdNaC1CR Test Number: 2 5ýý I D lgqoo
CdNaC1CR Test Number. _7,
.54vciL initial ii Final I initial II Final I initial II Final I Initial ii Final
/1/O/oo IiU-oo
/I1L),50 I0/o Page 7 ot 'I Initi I Imi t i aal Fin Initi H Final initial I Final Environmental TestingSolutions,_LLC Page 7 ci -I Day Concentration Parameter 3
4 5
6 PH (S.U.)
1b. Z
-Z
-3 DO (mgL 2
CONTROL Conductivty3 Temperature 743 Z.
45
-q-4 45 242.
PH (S.U.)
IF.V Z(.
Z 4.3 rsl-z-
&Z-8.?z.
847-z DO (g/U S.0 13.1 600 mg NaCIIL Conductivity I'195 (grahos/cr) 5 Temperature zi4 zq 800 mg NaCV/L Conductivity 0
nhsCm) l3/
/OIZ.
/821' Temperature 7..jqt-Z
-Z.2-.
q PH (S.U.)
P. 2C 64.)
6.0
.ZO 1000 mg NaCIIL Conductivity (Wnhos/cm) zzq Z13 2 -73, Temperature
-Z.Lq q,(
q
~
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7-44(2
-2 2q.2 Zj(
DO(g*
6.3 el 82
- 1200 mg NaCIJL Conductivity zI 244 (Muhos/cm)
~
.to24+
Temperature
~S 1
'.~2(I PH (S.U.)
.Z.
.I~t
~I 6
1400 mg NaCIIL Conductivity (junhos/cm) 2q 16q,
~
s Temperature 7-q.2-2q. S 2q.2-Z-(
Environmental Testing Solutions, LLC I I ZZ00
//11/00
Environmental 7Tsting Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water II I
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Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 +/- SA.75, SA. 75 : 0.38, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC25 +/- S A.0 0.45, as determined by USEPA for the method and endpoint) 2.0 1.5 I
U'-
1.0 0.5 0.0 II I
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I Warning and Control Limits Set According to 75th and 9 0 th Percentile CVs.
UV Treated Tests Since the coefficient of variation for the set of 7-d IC25 values (0.11) is less than the 75 t and 90t percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
4) a?
04
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O p
2 9
5
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Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
Control Limits (mean IC25 + 2 Standard Deviations)
Environmental Ttsting Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water 1.2 1.0 I-S0.8 in Q 0.6 0.4 k 0.2 I I I
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I Control Limits Set According to +/- 2 Standard Deviations.
°..
...................... °
°. "
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UV Treated Tests I
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2,t 30 10 0
Environmental
, sting Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephales promelas using Moderately Hard Synthetic Water I)
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UV Treated Tests 40K elk 4-a,?
0q ! O
ý O'k 0-0 1ý O4, 41 01-a,.
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Test date PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean PMSD)
......... Lower and Upper PMSD Bounds Lower PMSD Bound (10I' percentile) = 9.4%, Upper PMSD Bound (90'h percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
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A onmental Testing Solutions, LLC Dilution prep (mg/L)
I 4-__
"-'u C) 9 12 15 18 Diluent volume (mL) 994 991 988 985 982 I
Test organism in ormation:
Test information:
Organism age:
Zq"-fkes OiL Randomizing template:
t l7 Date and times organisms O-.0 t6 1, !Ib 7 ToI I -ý"
Incubator number:
z were born between:
I Organism source:
A& BF¶A 05-06-O2--
Artemia lot number:
7O1 Transfer bowl information:
pH =
Temperature =
Oven temperature:
(.0 C "7"140__
_Ž 0'c Drying time:
Daily feeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 1_ _-_
JO N A.) L 2
65-o?-oa-tow A67 i '?/0 3
O F~
z-0 -O L 0 % 1 1,: 6 ao:,
t s o I j
4 FI Control information:
Acceptance criteria Summary of test endpoints:
% Mortality:
2 20%
7-day LCSO
'717,1 Average weight per larvae:
1 q%(
Z
>0.25 mg/larvae NOEC QI ChV 46_
_1.
1 IC25
ýa.
Stock volume (mL) 1 6
Dilution preparation information:
Comments:
KC1 CCM number:
C4 "0
U.'0.4 Stock preparation:
50 g KC/L: Dissolve 50 g KCI in 1-L Deionized water Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promelas PpKCICR Test Number:
300 450 1 600 1 750 1 900 LOEC (too
Page 2 ot 5 knvironmental Testing Solutions, LLC PpKCICR Test Number:
-W
- x- "te~eareb Day 0
1 2
3 4
5 6
r==7 A--Pn weight (-)
B - Pan + Larvae wedght (mg)
Larvae weight (mg) = A - B CONTROL A
B C
D
/a
/0 /0
/0 Io 10 16 10 10
!0
/0 IC)
IV IC) I()
I
/0
/0
/0
/0 IC) /6 C
/0 a~i,/0
/0 L7 L o
/0 Yurvival and Growth Data 300M KCIIL E
IF G
H
/
I() 10 (C)
(0
/0
/0
/0 10
/0
/0
/0 to
/0 io
/0 z
/0 10 16
/0
/0
/0
/0 to
/0
/o q
/0 CW go)
.ýpe D It.4 1
450 M KCI/L I
J_ I K L
Jo
/0
/0
/0 10 10
/t lo e
/0 to (0
/0
/
c'
/0/6
/
1 J(
ti Ir A
Calculations and data reviewed:
.i I
Page 3 ot 5 Erviroumental Testing Solutions, LLC PpKCICR Test Number: _Ži Survival and Growth Data 60 750 m2 KCI/L go mg Day S*
M -1 N
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q R
T
-V W
6 I0
/0 0
1c)0 0
1()/I0
/0
/0 4 O
/0 S~Calculations and data reviewed:
Comments:----
I A/ o /
,A1 8 Z 5
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number:
PpKCICR # 2, UV Treated Test dates:
May 7-14, 2002 Dunnett's MSD value:
PMSD:
0.087 17.5 MSD =
Minimum Significant Difference PMSD =
Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions. LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control (detnenined Lower PMSD bound determined by USEPA (10th percentile) 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pirmephales growth in chronic reference toxicant tests-USEPA. 2000. Understanding and'Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Organisms obtained from Aquatic BioSyrtems. Inc Reveiwed by:
.& t
Concentrutioo (mg/L Repilcate IniRtaln iber of Fial numbe of A-Pan weight B-Pan + Lare Larvae weight (mg) Wetight/Ilninhoaber Mean mrvval Mean weight (mg) tlient of Pent reucton from Ka) tame trvae (m0) weight (ag)
- A-B oflarv (rag)
(%)
variation (V1 ontral (.)
A 10 9
14.545 19.310 4.765 0.4765 Control B
10 10 14.633 20.020 5.387 0.5387 97.5 0.4966 6.0 Not applicable C
10 10 14.867 19.840 4.973 0.4973 D
10 10 14.903 19.640 4.737 0.4737 E
10 10 14.720 19.880 5.160 0.5160 300 F
10 10 15.036 19.500 4.464 0.4464 97.5 0.4360 15.8 12.2 G
10 10 14.664 19.000 4.336 0.4336 H
10 9
15.059 18.540 3.481 0.3481 1
10 10 14.722 18.690 3.968 0.3968 450 1
10 10 14.851 18.860 4.009 0.4009 97.5 0.4120 6.6 17.0 K
10 10 14.791 18.770 3.979 0.3979 L
10 9
15.246 19.770 4.524 0.4524 M
10 9
14.713 17.840 3.127 0.3127 600 N
10 9
15.022 18.820 3.798 0.3798 95.0 0.3435 8.4 30.8 0
10 10 15.022 18.530 3.508 0.3508 P
10 10 14.692 18.000 3.308 0.3308 Q
10 6
15.065 18.440 3.375 0.3375 750 R
10 6
14.810 16.990 2.180 0.2180 52.5 0.2293 34.7 53.8 S
10 4
14.608 16.070 1.462 0.1462 T
10 5
14.607 16.760 2.153 0.2153 U
10 1
15.085 15.630 0.545 0.0545 900 V
10 4
14.916 16.460 1.544 0.1544 30.0 0.1210 37.9 75.6 W
10 4
14.817 16.300 1.483 0.1483 X
1 10 3
14.712 15.980 1.268 0.1268 05-07-02UV.xls
Environmental Testing Solutions, LLC Statistical Analyses Start Damt 5n7)82 End Date:
5/14/02 SamWle Da1.
Larval Fish Growth and Survival Test-7 Day Survval Test 11):
PpKCICR Sample ID:
REF-Re"Toxicant. UV-tUeamd Lab ID.
ETS-Env. Testing Solutions Sample Type:
KCL-Pot,*ssut chloride Protocol:
EPAF 91-EPA Freshwater Test Species:
PP.Pimephabe promelas Coonamts:
ConoaW1 I
2 3
4 DContrl 0.9000 1.0000 1.0000 1.0000 300 1.0000 1.0000 LM 0.9000 450 1.0000 1.0000
].0000 0.9000 600 0.9000 0.9000 1.0000 1.0000 750 0.6000 0.6000 0.4000 0.5000 900 0.1000 0.4000 0.400 0.3000 Transform: Arqan Square Root Rank 1-TaUed Conc-*mg/L Mean N-Mean Mean Min Max CV%
N Sumn Critical D-Costrol 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4
300 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4
18.00 10.00 450 0.9750 1.0000 L.3713 1.2490 1.4120 5.942 4
10.00 10.0 600 0.9500 0.9744 1.3305 1.2490 1.4120 7.072 4
16.00 10.00
- 750 0.5250 0.5395 0.8106 0.6847 0.8061 11.092 4
10.00 10.00 "900 0.3000 0.3077 0.5677 0.3210 0.6847 30.172 4
10.00 10.00 Number Total Re4p Number 1
40 1
40 I
40 2
40 19 40 20 40 Austihuy Teab Statistc Critical Skew Kurt Shapi.o-Wilk's Test indicaitz non-noemal disn'bution (p
- 0.01) 0.862000060 0.084
-1.017845934 0.379250916 Bas.tdt Test indicates equal variances (p - 0.73) 2.776863813 15.08631706 Hypothesis Test (I-lal, 05)
NOEC LOEC ChV TU Steers Many-One Rank Test 600 750 670.8203932 par*meter Value SE 95% FIducal LImits Slope 12.10974068 2.331844041 7.539326274 16.68015509 lelesept
-30.13650687 6.745375227
-43.35744257
.16.915571M7 TSCR 0.022501990 0.013S0M721 -0.004565055 0.049569051 Point Preolst mg/L 95% Fiduclal Limits ECOi 2.674 512.1560149 391.703859 5808151195 EC05 3.355 583.0137711 480.703891 640,2751492 EC]O 3.710 624.7116821 535.4309698 675.318892 EC15 3.964 654.5171571 575.2343058 700.768M006 EC20 4.158 679.2165533 608.3610921 722.3995151 EC25 4.326 701.1479503 637.6098112 742.2855737 EC40 4.747 759.6033144 712.0742551 801.1398016 ECS0 5.000 797.0907819 754.4481002 W46.0502775 EC60 5.253 836.428315 793.355763 900.2217852 EC75 5.674 906.1621171 852.5340065 1009.74882 EC80 5.042 935.4214269 875.2090525 1059.256191 ECas 6,036 970.7212707 901.5749537 1121.062246 EC90 6.282 1017.03508 935.0066861 1205.102395 EC95 6-645 1089.774769 905.6862065 1342.975512 EC99 7.326 1240.547294 1086.116912 1648.841103 Llkeilhood-Probil CentreL ChlSq Critical P-value Mu Sigma Iter 0.025 2.424613033 7.814724922 0.49 2.901507795 0.082578151 5
1.0 0.9.
o.8 0.7 0 0.6 0.5.
0.5 0.4" 0.3 0.2 0.1 0.0 Dote-Response Plot 0.8 0.7 0.6 0 0.5 p'0.4 1--
10 100 1000 10000 Dose mgIL
Environmental Testing Solutions, LLC Statistical Analyses Teat ID.
Ppt(CICIS Test ID:
PpKCICR Lab ID:
ETS-Ena.
Protocol:
EPAF 91 Larval Fish Growth and Sarvival Test-7 Day Growth Sample ED:
REF-Ref Toxicant. UV-trtated Testing Solutions Sample Type:
KCL-Potassiun chloride
-EPA Freshwater Test Species:
PP-Pimephales promodas Ceeao9gL 1
2 3
4 D-Conrol 0.4765 0.5387 0.4973 0.4737 300 0.5160 0.4464 0.4336 0.3481 450 0.3968 0.4009 0.3979 0.4524 600 0.3127 0.3798 0.3508 0.3308 750 0.3375 0.2180 0.1462 0.2153 900 0.0545 0.1544 0.1483 0.1268 Transform: Untransformed I.Ta~led Isotonic Cow0-ng2L Mean N-Ma Mean Min Max CV%
N t-Stat Critical MSD Mean N-Mean 0-Conbrol 0.4966 1.0000 0.4966 0.4737 0.5387 6.043 4
0.4966 1.0000 300 0.4360 0.8781 0-4360 0.3481 0.5160 15.801 4
1.678 2.410 0.0870 8.4360 0.8781 450 0.4120 0.8297 0.4120 0.3968 0.4524 6.551 4
2.343 2.410 0.0870 0.4120 0.8297
-600 0.3435 0.6918 0.3435 0.3127 0.3798 8.371 4
4.241 2.410 0.0870 0.3435 0.6918
-750 0.2293 0.4617 0.2293 0.1462 0.3375 34.656 4
7.409 2.410 0.0870 0.2293 0.4617
.900 0.1210 0.2437 0.1210 0.0545 0.1544 37.923 4
10.409 2.410 0.0870 0.1210 0.2437 Auxilinsry Tests Statirtie Critical Skew Kurt Shapim-Wilk's Tet indicates normal distribtuion (p > 0.01) 0.952484667 0.884 0.215608854 0.863392203 Bartletfs Teat indicates equal varinces (p = 0.32) 5.864162445 15.08631706 Hypothesas Tea (I-tall, M.85)
NOEC LOEC ChV TU MSDU MSDp MSB MSE F-Prob df Dunnetes Test 450 600 519.6152423 0.086950432 0.175109118 0.07932099 0.002603391 3.5E-08 5.18 Linear Interpolation (80 Resamples) peint Mg/L SD 95% CIAxp)
Skew IC05*
123.06 97.92 32.63 484.41 1.0553 ICI0*
246.12 92.86 65.26 520.64 0.4150 ICIS 387.14 92.53 87.11 536.37
-0.2871 1.0 IC20 482.33 64.69 136.47 577.07
-2.0787 0.9 IC25 536.72 29.90 446.28 627.33 0.2279 IC40 659.85 29.85 610.87 812.87 1.4573 0
IC50 725.03 33.50 648.90 836.91 0.4917 0.7 indicates IC estimate les than the lowest oancentration n
200 400 600 800 1000 D~ose-Response Plot 0.6.
0.5.
0 0.3 P- 0.2 0.1 01 1-tail, 0.05 level of significance Start Daw Sf1/02 End Da 5/14102 Sampe Datw Dose mg/L Dose-Respoa*
Plot
- frironmental Testing Solutions, LLC I
MHS Control Chemistry:
I Parameter Control Batch:
CS-okM o&-',-o
- o.
pH (s.u.)
.z DO (mg/L)
Conductivity 2(
(n*,hos/cm)
Alkalinity (mg CaCO3IL)
Hardness (mg CaCO 3iL)
Acceptance Criteria for Daily Chemistry:
iI I I I I I I I I,,,,,
- -'V.
PpKCICR Test Number: 4 OV/--TWa.Aret Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 300 mg KCI/L 840 - 890 450 mg KCI/L 1100- 1180 600 mg KCI/L 1390 - 1460 750 mg KCI/L 1660- 1710 900 mg KCI/L 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCI/L)
eage 5 ot 5 jr~ronu'ental Testing Solutions, LLC.....
PpKCCR Test Number:O O~v --'affp-0oq"o bO60oo 4,aqoo S'
Page 6 ot 5
,,n+nI Tafmno a1uutinn
T.T AT?
PpKC1CR Test Number: ILI Da Concentration Parameter 3
45 6
CONTROL pH (S.U.)
6..~.S.
2-3 DO (mg.2
-4.
Conductivity (AMImOS/CM) 305 z
310
.o Temperature zz q(
qq Zbo Zq.3' 7q.
pH (S.U.)
6.Z 3
e6 911 DO (mg/L).
(o.0 8-.
4500mg KCIIL Conductivity q
Temperature 2q~.3 z.10 L,
/.
A'LG 2q*
VI pH (S.U.)
jqZ.
86 A.1
-41 I&?
DO (mgi'L) ell
~-1 450Omg KCIJL Conductivity Temperature 44 i.3 AS4 7'/*
q.qL pH (S.U.)
q (3
1-q0 1~
60 gKILDO (mg/L)
.5
.0bj 900mgKCILConductivity 13 (Imos/cm 135~1 13 1 9 Temperature 41 ff Z.O Z
1 j5
'B (0c Ifllfl2
- 24.
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