ML022130393
| ML022130393 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 07/11/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML022130393 (122) | |
Text
Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 July 11, 2002 State of Tennessee Department of Environment and Conservation
.Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Please find enclosed the Discharge Monitoring Report (DMR) of June 2002 for Sequoyah. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 PnntedOw -ecyled -oer
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01)
Address BOX 2000 (INTEROFFICE SB-2A) TN0026450 101 G F - FINAL
_ ___$_S.LY - DAISY _TM 3738B PERMIT NUMBER DISCHARGE NU)MBR DIFFUSER DISCHARGE Facili A- _ _R_
Location HAMILJON COUN . . lA(3tNIT/TPDINr(_' PPI(Dlrf EFFLUENT FFAro Mm DAY VFAr Me DAY NO DISCHARGE ATTN: Diedre B. Nida NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING I QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 29.9 (04) 0 30 / 30 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 ,PERIMIJ 3G*. K REG INSTREAM MONITORING DERMIT. ... REQ DAILY MX _____PRI __
TEMPERATURE, WATER DEG. SAMPLE * **42.6 (04) 0 30 / 30 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 0 PER~MIT7 POT DEG. C. SE CK REG EFFLUENT GROSS VALUE REQUIREMENT. PER~MIT____
PH SAMPLE 7.2 ********
MEASUREMENT 7.3(12) 0 8/30 GRAB 00400 1 0 0 ~ PERMITK~ 6. ~.'0 9**(*~ SU WEEKL~tY G RA B EFFLUENT GROSS VALUE REQUkEM** , .MIN IMUMMAX ... I M SOLIDS, TOTAL SUSPENDED SAMPLE 6 8 0 4 / 30 GRAB MEASUREMENT (19) 00530 1 0 0 ' PE RMI 'P,* MGIL V i EVKL% G.RAB EFFLUENT GROSS VALUE REQUJIREMENST MO AVG DAILYM)
OIL AND GREASE SAMPLE <5 <5 0 4/ 30 GRAB MEASUREMENT (9) 00556 1 0 0 P .. .iRMIT%>~ MGIL .WEEKILY GRAB EFFLUENT GROSS VALUE 'REQUIREMENT[ MO. AV0G DAIL MX____ ___
FLOW, IN CONDUIT OR THRU SAMPLE 1787 (03) 0 30/30 RCORDR MEASUREMENT TREATMENT PLANT 50050 1 0 0 W:JPERMIT~~ _ 4 MGD , ,C;RDF, C.NTIN EFFLUENT GROSS VALUE <>.> DAILY~I~ MX US CHLORINE, TOTAL RESIDUAL SAMPLE **.*6(*9 G********
MEASUREMENT <0.008 0.016 (19) 0 39/30 GRAB 50060 1 0 0 . PER~MIT ,L 0. MG/ WEK
.EFFLUENTGROSS VALUE ,RENJRSgN ,~ ' ~~ ~ .3>P'*' 0O8'Ž MAX M/ WE~ S,' CL NAME/fITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my . . t__ ,- * / TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or 1U LA- '
persons who manage the system, or those persons directly responsible for gathering the Environmenfal Supervisor Site Vice President information, the information submitted is , to the best of my knowledge and belief, true, accurate, EnvironmentalSupervisor _ 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
TYEDORPRNTDOFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I___________________________I___
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No closed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.053 mg/L--limit 0.100mg/L) 2. CL-363 (max. calc. conc. was 0.012 mg/L--limit 0.100mg/L) 3. PCL 222/PCL-401 (max. calc. conc. was 0.0188mg/L--Iimit 0.100mg/L) 4. H-130M (max. calc. conc. was 0.022mg/L--limit 0.050mg/L)
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
Address BOX 2000 (SUBR 01) OMB No. 2040-0004
.-f. IfNTFROQFFICE SB-2A T 26450 1 101 G F - FINAL
_ _-_ SD.y-AISY TN 37334 PERMIT NUMBER IDISCHARGE NUMBER DIFFUSER DISCHARGE Facility TVA - SEQUOYAH NUCLfARP2LN_
Location HMILTQL. O _.. . EFFLUENT I YEAR MQ I DAY.I I YFAR IMhdQ I DAYI ATITN: Diedre B. Nida From l 02 06 _01 I To 02 1 06t1i30 NO DISCHARGE -]
NOTE; Rp.ad ind*rirnlinnur huufnra r'nmnhptinn thir fnrm PARAMETER QUANTITY OR LOADING J QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE ***0.1 (62) 0 30/*30 CALCTD CHANGE MEASUREMENT 82234 1 0 0 PERM : .IT . . 2Q DEG..CAL...
EFFLUENT GROSS VALUE /HR TEMP. DIFF. BETWEEN SAMP. & SAMPLE 1.9* (04) 0 30 / 30 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S 0 PE~RMIT r**** DE.C9LQD EFFLUENT GROSS VALUELE SAMPLE MEASUREMENT MEASUREMENT SAMPLE REQUIREMENT. (19 SAMPLE REQUIREMENTh MEASUREMENT BORON,TOTAL ~SAMPLE
- 0 0 0 MEASUREMENT EFFLUENN GRS VALU E<0.2 (19) 0 1 /30 GRAB 0102 ~00 p~MI ~REPORT' ~REPORT RE~PORT MG/L GRAB' SAMPLE ____
MEASUREMENT REQUIREMEN-1 ~ r ~ .
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and aft attachments were prepared underm '/ TELEPHONE DATE
-direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alf attachmentshere)
CCW data for June 2002 is attached.
itr-A rorllmOZ' M20- rj:V 3,l9) t",feVibULed. ns H~ be$UI
- USeu: Page 2 of 2
CCW Data for June 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
June 4, 2002 < 0.5 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
June 4, 2002 0.6 Page 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 2000 (INTEROFFICE SB-2A. TN0026450 101 T F - FINAL
---_ 5_D_.
S__DD_
- D__
Y TNPA-7 - -384 F ERMIT NUMBER DIFFUSER DISCHARGE Facility iVA -UCL LQY LAPNT DISCHARGE NUMBER IVI0NITOlIN(. PFRIOD EFFLUENT Location HAMILTON __COUNL___
-YEAR- I.MOb I AY I I YEAR.1 MC0 I DAYI From 021I041I01 1 ToJ02 1061301 .*.NO DISCHARGE F
- ATTN: Diedre B. Nida NOTE: Read instructions before completing this form.
PARAMETER QUANTITY OR LOADING NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS ANALYSIS
+ -I IC25 STATRE 7DAY CHR +
1/91 COMPOS CERIODAPHNIA (23) 0 TRP3B 1 0 0 IERCENT COMPOS EFFLUENT GROSS VALUE QURT' ERLY IC25 STATRE 7DAY CHR SAMPLE ********
COMPOS MEASUREMENT
>100 ........ (23) 0 1/9]
PIMEPHALES ** I** ** ** **
TRP6C 1 0 0 ":RCENT Q-UART COMPOS EFFLUENT GROSS VALUE ERLY
. U>
w 2,
SAMPLE MEASUREMENT MEASUREMENT I I SAMPLE MEASUREMENT I I NAME/FITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel
- , ,t I ii'f . TELEPHONE DATE Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or .
persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor 02 07 11 information, the information subrttted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TY P ED OR PR INTEDO F I E OR A T O I E A ENA R AN M EY AR MD Y COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
Toxicity was sampled from 5/5/02 to 5/10/02. The report is attached.
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 2000 (INTEROFFICE SB-2A. TNo02640 I03 F - FINAL
-. DDY - DAISYT T_ M37a84 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Focilitv !VA - SEQUOYAH NUCLEAR PI _ .I.. ._..
Location HAMJLTON COIy--_ -
I MC)NIT(')FIN(- PF~IC)n _ EFFLUENT YFAPl MO' rm DA l Yr ý Mn I UAYI From 02 06 01I Tol 02 Io .*. NO DISCHARGE E:]
ATTN: Diedre B. Nida NOTE: Read instructions before completingt this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE **7.2 8.7********
MEASUREMENT (12) 00400 1 0 0 ,PERMITQ >* -*~o~ 9,0***2~4~ SU THREE/ GA EFLETGOSVLEMNMMMAXIMUM WEEK ___
SOLIDS, TOTAL SUSPENDED SAMPLE 73 113 (26) 8 12 0 4/30 GRAB MEASUREMENT 0500 1 0PE~fMNIT (19 0T264/30WEKL GRAB 00530~~~ 1 01050 LBS/DY 30~~ A 100JM/ E~<Y QA EFFLUENT GROSS VALUE REQUIREMENTY:yIX G DAI___ ___
OIL AND GREASE SAMPLE MEASUREMENT <44 <49 (26) <5 <5 (9
0 4/30 GRAB EFFLUENT GRS VAU PERIRT I9 1) 0 /0 GA 0055 120050 LBS/DY 15 o MG/L WEEKLY RA EFFLUENT_____ GRSSVAUE____EMN MO.0 AVG D5AILY MX' MO V DAILY MX _ ____
FLOW, IN CONDUIT OR THRU SAMPLE 1.035 1.193 (03) 0 30/30 TOTAL TREATMENT PLANT MEASUREMENT 50050 GRo 0V PERMIT RPORr'* REPORT...
. MGD 1.....DAILY TQTAL REQUIREMENT .
SAMPLE MEASUREMENT REQUIREMEN1T ' < '>j 4 ______________
SAMPLE MEASUREMENT SAMPLE MEASUREMENT REQUIREMENT, ~ ~
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER IlCetf under penalty of law that this document andcall attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel
.IL)TELEPHONE DATE Richard T. Purcell properly gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the Environmental Supervisor Site Vice President information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
AREA I NUMBER YEAR MO DArY TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
EPA Form 3320-1 (REV 3/99) Previous editions may 1be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Locationif Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 2000
-_ - - (INTEROFFICE S8-2_AA .. TN06450O17 F - FINAL
-_ - - __SODQ~Y - DAISYL -_ TN a37384 - -
Facility TVA --- EQC-Y-HN_.CLEAR.LL PERMIT NUMBER DISCH 1 RGE NUMBER METAL CLEANING WASTE POND Location HAAMILTQ_1_C____U . . . . . . . MONITQr?IN(-: PERIOD EFFLUENT YEAR L.LM DAY I I YEAR* I O DA From 02 106 o1 1 To 102 1 06 j30 ...NO DISCHARGE IF ...
ATTN: Diedre B. Nida NOTE: Read instructions before con I
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER law that this document and all attachments were prepared under my
-Irir-etion nr Stinlrri-inn ijn accordance with a system designed to assure that qualified personnel Richard T. Purcell uate the information submitted. Based on my inquiry of the person or system, or those persons directly responsible for gathering the on submitted is, to the best of my knowledge and belief, true, accurate, Site Vice President athat there are significant penalties for submitting false information, linciuaing tme I fine and Imprisonment for knowing violations.
TYPED OR PRINTED COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No phosphate bearing chemicals were employed. No Discharge this Period rrovtuua uuirtorts may beae used useo Page 1 of 1 EPAf r-orm 3320,-U-1 kR-V 199I)IU P~revious eumIuOIs may Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 2000 (INTEROFFICE SB-2A. TN0026450 I1 110 G F - FINAL
- _ . OQDY-_DAILSY TN 37M384 PERMIT NUMBER IDISCHARGE NUMBE RECYCLED COOLING WATER Facility TA - EQUQY Ht_NUCLEAR PLANT Locotion__-AMILTON COgUN]y .. Mh-NITflDIN(FJ PfZDlnrt I EFFLUENT ATTN: Diedre B, Nida From Frm 1YER02I 040To 6- 0-11 Tol1o2 1 06 I8A1O
-130 ... NO DISCHARGE .XX..
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT (12) 00400 1 0 0 PERMIT )
- ';.*i**Y** 6.0 9.0 SUDAILY GRAB EFFLUENT GROSS VALUE 9UEMN 'f. ,MIMUMAI M________
-- MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE MEASUREMENT (19) 00530 1 0 0 PERMIT > *** 3/4Ž~**~ MGL < AIYC PO EFFLUENT GROSS VALUE :QUIREMENT AL:_______:
OIL AND GREASE SAMPLE MEASUREMENT (19) 00556 1 0 0 4PERMIT 4*~~.MIL~~~ALY qMO EFFLUENT GROSS VALUE REQUIREMENT- 15,,. MGAILAILYX'MP COPPER, TOTAL (AS CU) SAMPLE
- MEASUREMENT (19) 01042 1 0 0 ~ PERMIT < . **f f ~ ~1.0 MGIL £ _j_AIY~ -COM1PQ&
EFFLUENT GROSS VALUE REQUIRE3MENT 2, DAILY X IRON, TOTAL (AS FE) SAMPLE
- MEASUREMENT (19) 01045 1 0 0 '** PERMIT
........ 1Os'
. MGIL DAILY QQMP..
EFFLUENT GROSS VALUE REURMN 1. DAIL COMPOS PHOSPHORUS, TOTAL (AS P) SAMPLE (19)
MEASUREMENT (19) 00665 1 0 0 'PERI9MT -*... .
EFFLUENT GROSS VALUE REQUIREMENT' 1. MGIL DAILY COMPOS DAILY M FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 R T R)'.*4EPORT MGD ..... ALCTD CAY EFFLUENT GROSS VALUE REQUIREMENT. MO AVG~ DALY NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ~I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed (I .4,/)TELEPHONE DATE to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or C.y 1 ,
persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor information, the information submitted is. to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER TYPED O R PRINTED O F C RORO AUTHORIZED U H R Z DAGENT A E TA ARE I N ME YEAR E R MO M DAYA COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)
No Discharge this Period EPA* Form 3,320-1 (RilEV ,./19) I-re vious editions may be usea Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 2000 S. (INTEROFFICE SB-2A.
. . __S
. DY &_Y=D ISY_. -_TN 37"384 TN 6450 1 F - FINAL
- - PERMIT NUMBER IIDISCHARGE NUIMER:
BACKWASH Facility_ T\VA -EQUOYAH NU.QL.ERPL*N** - -
Location HAMILTCQOU.N _ EFFLUENT ATTN: Dledre B. Nida From o Qo Y Io Yo A M2II DAY ... NO DISCHARGE L NOTE: Read instructions before comolefino this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE * **0 * (00*0 /30 VISUAL MEASUREMENT (00) 01345 1 0 0 PERMIT r **** '*** R P T PAS0EE VISUAL EFFLUENT GROSS VALUE R UIE NT FAIL=1 PFMT___
SAMPLE MOTTLPRI MEASUREMENT PERMIT REASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT PERMIT MEASUREMENT REQUIEMENT SAMPLE MEASUREMENT REQUIREMENT <4 NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Cenify under penalty of law that this document ard all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel
- 1 - 1 l TELEPHONE DATE Richard T. Purcell property gather and evaluate the information submitted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor Site Vice President information, the information submitted is. to the best of my knowledge and belief, true accurate, 423 843-6700 02 07 11 and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations.
TYPED OR PRINTED OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ottachments here)
Operations is required once per shift to perform visual inspections.
E:PA/ Form 3320-1"U° (RE::V 3,'/99)J P'reviouseditions may be used Page I of I
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved, Nome TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No. 2040-0004 Address BOX 200 (INTEROFFICE SB-2A. TN0026450 G F- FINAL
_ __. _QDD__-_DALSL -_M 37384.......
PERMIT NUMBER DISCHARGE NUMBER BACKWASH Facility TVA - SEQUOYA NUCLFRLANT Location J-H AM TITN _.CO UNT-- _ ._M_)NITlNP_ .___lf_ EFFLUENT From I1YEAR 02 I06MC D-AY 01 I To 1 02 YIARI MCID)AyI 06 -- 370 ATTN: Diedre B. Nida
- NO DISCHARGE L[_i
- NOTE: Read instructions before comoletino this form.
PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE
- 0 (00) 1 /*30 VISUAL MEASUREMENT (00) 01345 1 0 0 PERMIT REPORT. PASS=0 SEE VISUAL EFFLUENT GROSS VALUE REQUIREMENT. FAIL=1 PERMIT SAMPLE MEASUREMENT SAMPLE '___'_
MEASUREMENT SAMPLE PERMIT MEASUREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE REQUIREMENT MEASUREMENT k SAMPLE MEASUREMENT (PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my fl / t TELEPHONE DATE direction
- or supervision in accordance with a system designed to assure that qualified personnel CQA4 Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or .Y1 I Sersons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor i complete. I am aware that there are significant penalties for submitting false and information, SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations.
I OFFICER OR AUTHORIZED AGENT AREA RAMR I NUMBER YEAR MO DAY M
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all ottochments hefe)
Operations is required once per shift to perform visual inspections.
EPA Form 3320-1U- (R'ElV 3/99) P-revious editions may be useo Page 1 of 1
PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.
Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) 0MB No. 2040-0004 Address BOX 2000 (INTEROFFICE SB-2Aý - TN0026450 1 118 G ] FF- FINAL SO5DDY -__DA_.Y- - TN 37384...
Facility TVA - SEQUOYAH NUCLEAR2.NT PERMIT NUMBER IIDISCHARGE NUM WASTEWATER & STORM WATER I =
Location HAMI LTQONOTY hAnr-jITnDIfI\l*r- PPDIrt'- I EFFLUENT ATTN: Diedre B. Nida From 1F 02 MOLVroAr 06 101 . 1 To102 I"DATYO T YA" o1061301 I AY NO DISCHARGE 5 ...
NOTE: Read instructions befnre, Onmno.tino thi.* fnrm PARAMETER QUANTITY OR LOADING 1 QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE *******
MEASUREMENT (19) 00300 1 0 0 PERMIT 2.0 MG/L TWICE/ GR9AB EFFLUENT GROSS VALUE REQUIREMENT- .. .DAILY 0 MN ' WEEK_._____
SOLIDS, TOTAL SUSPENDED SAMPLE ***** **'
MEASUREMENT (19) 00530 1 0 0 ~ PERMIT W *;*. ***MGLTI! RA EFFLUENT GROSS VALUE IE...T',; .... ,
->....._________"....DAILY MX ______..... WEEK _______
SOLIDS, SETTLEABLE SAMPLE **
MEASUREMENT (25) 00545 1 0 0 PERMIT ~ ****~~~. ~* ~ ~ ~ MI N~ IA EFFLUENT GROSS VALUE IEPiIRE*.h :,,DAILY X _ ___ MON* _,_,__
FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT (03) 50050 1 0 0 PERMIT REPORT REPORT MGD ON-CE ESTIMA EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MVX BATCH ____
SAMPLE MEASUREMENT REQUIREMENT SAMPLE MEASUREMENT SPERMIT ' .. ~~, V Vi REQUIREMENT V SAMPLE MEASUREMENT PE~RMIT .
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared underm f/ ' / TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel Richard T. Purcell properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the Environmental Supervisor information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-6700 02 07 11 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED including the possibility of fine and imprisonment for knowing violations. O OFCR.RATRIED AGENT AREA INUMBER YEAR MO DA COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oil attachments here)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions may be used Page I of 1
June 26, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, MAY, 2002 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report is included for your records. The report provides results of compliance testing using fathead minnows and daphnids.
Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent. Daphnid reproduction was significantly different from control based on results from the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report. ..
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN May 2002m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE
SUMMARY
- 1) Facility/Discharger: Sequoyah Nuclear Plant/TVA Report Date: June 26, 2002
- 4) Type of Facility: Nuclear-fueled electric generating plant
- 5) Design Flow (MGD): 3
- 7) 1Q10:2,992.4
- 8) Outfall Tested: 10_1 9) Dates Sampled: May 5-10, 2002
- 10) Average flow on day(s) sampled (MGD): 851, 869, 875
- 11) Pertinent site conditions: No unusual conditions reported. *
- 12) Test Dates: May 7-14, 2002 13) Test Type: Short-term Chronic-definitive
- 14) Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15) Concentrations Tested (%): Outfall 101: 10.98, 22, 43.9, 72, 100 Intake: 100 Pimephalespromelas:UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16) Permit Limit Endpoint (%): Outfall 101: IC5 = 43.9
- 17) Test Results (%): Outfall 101: Pimephalespromelas: IQC 2 5 > 100 Ceriodaphniadubia: IC 2 5_> 100 UV treated Outfall 101: Pimephalespromelas:IC 5> 100
- 18) Facility
Contact:
Diedre B. Nida 19) Phone #: (423) 843-6700
- 20) Consultant/Testing Lab Name: Environmental Testing Solutions, LLC 2 1 ) Lab
Contact:
Jim Sumner 22) Phone #: (828) 862-8193 TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- Production/operation data will be provided upon request.
- 22) Notes: Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent.
Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent.
Daphnid reproduction was significantly different from control based on result of the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms. These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
METHODS
SUMMARY
Samples
- 1) Sampling Point: Outfall 101, Intake
- 2) Sample Type: Composite
- 3) Sample Information:
Sample Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp. TRC* Used (MM/DD/YY Time) (MM/DD/YY Time) (°C) (mg/L) (MM/DD/YY Time) 101 1105/06/0205/05/02 1450 Intake 1350to 05/05/02 1509 to 05/06/02 1409 05/07/02 1155 05/07/02 1155
{05/07/02
- 0. 3 '
0.3
<0.10
<0.10 05/08/02 1458 05/07/02 1450 1458 05/08/02 1450 05/07/02 1224t09____ 05/09/02 1446 101 0105/08/0205/07/02 1225 1125to 05/09/02 1112 0.3' <0.10 05/10/02 1446 05/09/02 1440 05/09/02 1112 0.3 <0.10 05/09/02 1446 Intake 05/07/02 1241 to 05/08/02 1141 05/09/02_11 1 1 05/10/02 1440 05/11/02 1511 101 05/09/02 1318 to 05/11/02 1145 1.8' <0.10 05/12/02 1518 05/10/02 1218 05/13/02 1514 05/11/02 1511 Intake 05/09/02 1333 to 05/11/02 1145 1.8 <0.10 05/12/02 1518 05/10/02 1233 05/13/02 1514
- Total residual chlorine.
t Collected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival.
2
- 4) Sample manipulation:
Non-treated samples (compliance test) from Outfall 101 and Intake were warmed to test temperature (25.0 +1.00 C) in a warm water bath.
Aliquots of Outfall 101 and intake samples used for the side by side UV treated fathead minnow test were warmed to 24 0 C in a warm water bath and held in a 40 Watt Rainbow Lifeguard UV Sterilizer for 2 minutes, prior to exposure of test organisms.
Test Organisms Pimephalespromelas Ceriodaphniadubia
- 1) Source: Aquatic BioSystems, Inc. In-house culture
- 2) Age: 23.0-26.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> < 24 hours2.777778e-4 days <br />0.00667 hours <br />3.968254e-5 weeks <br />9.132e-6 months <br /> Test Method Summary (See Appendix A for additional information)
Pimephalespromelas Ceriodaphniadubia
- 1) Test Conditions Static, renewal Static, renewal
- 2) Test Duration 7 days Until at least 60% of control females have 3 broods
- 3) Dilution/Control Moderately Hard Moderately Hard Water Synthetic Water Synthetic Water
- 4) Number Replicates 4 10
- 5) Animals per Replicate 10 I
- 6) Test Initiation: (Date/Time)
Outfall 101 05/07/02-1458 EDT 05/07/02-1249 EDT UV Treated Outfall 101 05/07/02-1600 EDT
- 7) Test Termination: (Date/Time)
Outfall 101 05/14/02-1409 EDT 05/14/02-1300 EDT UV Treated Outfall 101 05/14/02-1436 EDT
- 8) Test Temperature: Outfall 101: Mean =24.5 'C Mean = 24.7'C (24.2-25.1 0 C) (24.2-25.40 C)
Test Temperature: UV-Treated Outfall 101: Mean = 24.5°C (24.2-25.3°C) 3
- 9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)
- 1) Results of a Pimephales promelas Chronic/7-d Toxicity Test (Genus) (Species) (Type/Duration)
Conducted 05/07/02 05/14/02 Using Effluent From Outfall 101.
(mm/dd/yy) (mm/dd/yy) (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4* 5 6 7 Control 100 100 100 100 100 100 98 10.98% Effluent 100 98 95 95 95 95 95 22% Effluent 100 95 95 95 95 95 95 43.9% Effluent 100 100 100 100 100 98 98 72% Effluent 100 100 100 100 100 98 98 100% Effluent 100 98 98 98 98 98 97' Intake 100 100 100 98 88 75 68
- 96-Hour LC5 0 Value: > 100% Calculated TU Estimates: t < 1.0 TUa 95% Confidence Limits: Permit Limit: 1.0 TUa Upper Limit NA Lower Limit NA IC 2 5 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2 3 4 j Mean Control 0.448 0.476 0.405 0.432 0.440 10.98% Effluent 0.562 0.361 0.557 0.502 0.496 22% Effluent 0.497 0.463 0.353 0.472 0.446 43.9% Effluent 0.583 0.448 0.406 0.552 0.497 72% Effluent 0.421 0.486 0.519 0.507 0.483 100% Effluent 0.546 0.624 0.492 0.536 0.549 Intake 0.478 0.389 0.125 0.463 0.364 IC 25 Value: > 100% Calculated TU Estimates: t < 1.0 TUc 95% Confidence Limits: Permit Limit: 1.0 TUc Upper Limit = N/A Lower Limit = N/A One fish was lost during termination. The test concentration was initiated with 39 fish instead of 40 for statistical purposes.
tTUa = 100/LC5 0: TUc = 100/IC 25 5
TOXICITY TEST RESULTS
- 2) Results of a Ceriodaphnia dubia Chronic/ 3-brood Toxicity Test (Genus) (Species) (Type/Duration)
Conducted 05/07/02 05/14/02 Using Effluent From Outfall 101.
(mm/dd/vv' (mm/dd/vv) (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4* 5 6 7 Control 100 100 100 100 100 100 100 6.25% Effluent 100 100 100 100 100 100 100 12.5% Effluent 100 100 100 100 100 100 100 25% Effluent 100 100 100 100 100 100 100 50% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100
- 96-Hour LC 50 Value: > 100% Calculated TU Estimates: t < 1.0 TUa 95% Confidence Limits Permit Limit: 1.0 TUa Upper Limit = NA Lower Limit = NA Reproduction (#young/female/7 days) Data Test Solutions Replicates 1 2 3 4 5 6 7 8 9 10 Mean Control 25 28 31 32 24 26 25 25 29 31 27.6 10.98% Effluent 26 28 28 29 29 31 27 26 32 30 28.6 22% Effluent 26 31 26 32 29 31 30 27 25 28 28.5 43.9% Effluent 25 28 26 31 29 34 28 28 30 31 29.0 72% Effluent 27 26 30 27 31 27 27 30 32 34 29.1 100% Effluent 30 31 28 30 28 29 33 35 33 32 30.9 Intake 30 22 24 21 15 14 23 18 26 27 22.0 IC 25 Value: > 100% Calculated TU Estimates: t < 1.0 TUc 95% Confidence Limits: Permit Limit: 1.0 TUc Upper Limit = N/A Lower Limit = N/A tTUa = 100/LC50 : TUc = 100/ IC 25 RFFFRFNCE S.. . . . . . . .
TOXICANT TEST RESULTS (See Aopendixes A and D)
. . . .. . . .. . . . . . . . . . . . . . \ . . . . . _- _7 Species Date Time I Duration Toxicant Results (I Pimephalespromelas 05/07/02 1538 7-days KC1 544.6 mg/L (IC 2 5 )
Ceriodaphniadubia 05/07/02 1220 7-days NaC1 1049.2 mg/L (IC 25) 6
TOXICITY TEST RESULTS UV-TREATED (See Appendix B for Bench Sheets)
- 3) Results of a Pimephales promelas Chronic/7-d Toxicity Test (Genus) (Species) (Type/Duration)
Conducted 05/07/02 05/14/02 Using Effluent From Outfall 101, UV treated (mrmdddvw) (mm/dd/yy) (number)
Percent Surviving Test Solutions (time intervals used - days) 1 2 3 4* 5 6 7 Control 100 100 100 100 100 98 98 10.98% Effluent 100 100 100 100 100 95 95 22% Effluent 100 100 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 98 98 Intake 100 100 100 100 100 100 100
- 96-Hour LC5 0 Value: > 100% Calculated TU Estimates: ? < 1.0 TUa 95% Confidence Limits: Permit Limit: 1.0 TUa Upper Limit NA Lower Limit NA IC2 5 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2 3 4 Mean Control 0.494 0.446 0.475 0.479 0.473 10.98% Effluent 0.377 0.477 0.487 0.336 0.419 22% Effluent 0.504 0.484 0.508 0.541 0.509 43.9% Effluent 0.512 0.516 0.493 0.613 0.533 72% Effluent 0.458 0.480 0.440 0.443 0.455 100% Effluent 0.474 0.480 0.420 0.520 0.473 Intake 0.477 0.436 0.447 0.496 0.464 IC 25 Value: > 100% Calculated TU Estimates: 1 < 1.0 TUc 95% Confidence Limits: Permit Limit: N/A Upper Limit = N/A Lower Limit = N/A TUa = 100/LC5 0: TUc = 100/IC25 REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D)
Species Date Time Duration Toxicant Results (IC 25)
Pimephalespromnelas 05/07/02 1538 7-days KC1 536.7 mg/L (IC 25)
UV- Treated 7
SUMMARY
/CONCLUSIONS Outfall 101 samples collected May 5-10, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows to intake samples resulted in no significant differences from controls during this study period, although intake minnow survival was only 68 percent. Daphnid reproduction was significantly different from control based on result of the Homoscedastic t-Test.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
8
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1) Pimephalespromelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2) Ceriodaphniadubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL
- 1) Pimephalespromelas None
- 2) Ceriodaphniadubia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1) Pimephalespromelas None
- 2) Ceriodaphniadubia None 9
PHYSICAL AND CHEMICAL METHODS
- 1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2) Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3) Temperature was measured using EPA Method 170.1.
- 5) The pH was measured EPA Method 150.1.
- 6) Conductance was measured EPA 120.1.
- 7) Alkalinity was measured using EPA 310.1.
- 8) Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1) Test Type: 7-day chronic tests with results expressed as IC 25 values in g KC1.
- 3) Dilution Water Used: Moderately hard synthetic water.
- 4) Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES
- 1) NPDES Permit No. TN0026450.
- 2) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).
- 3) Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
10
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Cuprostat Date Sodium Towerbrom PCL-222 PCL-401 PF hypochiorite mg/L Azole mg/L TRC mg/L TRC tin/gL mg/L
__________Phosphate Copolymer 03/12/1998 0,016 -
03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 -
03/17/1998 0.020 -
03/18/1998 0.018 09/08/1998 0.015 - 0.014 0.005 09/09/1998 0.003 - 0.031 0.011 09/10/1998 0.014 - 0.060 0.021 09/11/1998 0.013 - 0.055 0.019 09/12/1998 <0.001 - 0.044 0.015 09/13/1998 <0.001 - 0.044 0.015 09/14/1998 0.008 - 0.044 0.015 02/22/1999 <0.00:1 02/23/1999 0.005 02/24/1999 0.009 -
02/25/1999 0.012 02/26/1999 0.008 02/27/1999 <60.001 02/28/1999 <0.001 08/18/1999 - 0.015 0.069 0.024 08/19/1999 08/20/1999 0.012 0.023 0.070 0.024 0.120 08/21/1999 - 0.068 0.024 08/22/1999 - 0.022 0.068 0.024 08/23/1999 - 0.025 0.068. 0.024 08/24/1999 - 0.016 0.067 0.023
Table B- I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat hypochio~rite PF mg/L TRC mg/L TRC mg/L mg/L mg/L mg/L Azolt Phosphate' Copolymer DMIAD 01/31/2000 - < 0.002 0.026 0.009 -!
02/01/2000 - 0.011 0.026 0.028 02/02/2000 - 0.028 0.026 0.009 0.006" 02/03/2000 - 0.008 0.027 0.009 -'.
02/04/2000 - 0.006 0.027 0.009 0.005 0.109 02/05/2000 -< 0.002 0.027 0.009 -. -
02/06/2000 - < 0.002 0.027 0.009 -
07/26/2000 -< 0.0057 0.055 0.019 ,
07/27/2000 - 0.019 0.055 0.019 -
07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 07/29/2000 -< 0.0088 0.055 0.019 -
07/30/2000 - < 0.0076 0.055 0.019 07/31/2000 -< 0.0152 0.055 0.019 0-006 08/01/2000 -< 0.0141 0,055 0.019 0.005 12/11/2000 - 0.0143 0.025 0.020 0.005 12/12/2000 - 0.0092 0.025 0.020 0.00OO5y" 12/13/2000 - <0.0120 0.025 0.020 12/14/2000 -< 0.0087 0,025 0.020 -
12/15/2000 - 0.0120 0.025 0.020 0.005 12/16/2000 -< 0.0036 0.025 0.020 12/17/2000 - <0.0036 0.025 0.0201 ,.
08/26/2001 - 0.017 0.06 0.021 -i 0.006 08/27/2001 - <0.0096 0.06 0.021 0.005 08/28/2001 - <0.0085 0.06 - 0.021 ,
08/29/2001 - <0.0094 0.059 0.020 0:oO5 08/30/2001 - <0.0123 0,06 0.021 0.005 08/31/2001 - <0.005 0.059 0.020 -
11/25/2001 - <0.0044 ..- -
11/26/2001 - <0.0119 0.024 0.02 0.005, 11/27/2001 - 0.0137 0.023 0.019 0.007' 11/28/2001 - <0.0089 0.022 - 0.019 , 0.006 -'
11/29/2001 - 0.0132 0.024 0.02 0M007 11/30/2001 -< 0.0043 0.024 0.02 . -
12/09/2001 - <0.0042 , -
12/10/2001 - <0.0042 ,- - ,,
12/11/2001 - <0.0104 -", -
12/12/2001 - 0.0128 0.02 0.008 12/13/2001 - <0.0088 0.024, 0.02 ,
12/14/2001 - 0.0134 0,024 1 0.02 0.007 j
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-May 10, 2002 Date Sodium Towerbrom , PCL-222 PCL-401 CL-363 Cuprostat hypochlorite mg/L TRC mg/L mg/L ig(L. .: PF mg/L mg/L TRC Phosphate Copolymer DMAD Azole 1/02/2002 - < 0.0079 0.023 0.02 0.006 1/03/2002 < 0.0042 0.023 0.014 1/04/2002 - 0.0124 0.024 0.014 0.009 1/05/2002 -< 0.0042 -.
1/06/2002 - <0.0042 -
1/07/2002 - < 0.0089 0.024 0.014 0.0066 2/24/2002 - <0.004 - . - -"
2/25/2002 - < 0.004 0.023 0.023 2/26/2002 - 0.0143 0.023 0.023 {0.007 2/27/2002 - <0.0041 0.023 0.023 -
2/28/2002 -< 0.0041 0.024 0.008 . (*
3/01/2002 - <0.0041 0.024 0.008 - *,
5/05/2002 - <0.0070 - -
5/06/2002 - <0.0106 0.058 0.02 O.0l4 5/07/2002 - <0.0154 0.058 0.02 0.0 15 :
5/08/2002 - <0.0150 0.056 0.019 5/09/2002 - <0.0122 0.057 0.02 0.014 5/10/2002 - <1.0189 0.056 0.019 - -
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphniadubia Tests, Sequoyah Nuclear Plant Effluent (SQN),
Outfall 101, May 5-14, 2002 Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (°C) (oC) (mg/L) (mg/L) S.U. S.U. (ptmhos) mg/L CaCO 3 mg/L (mg/L)
CaC03 Fathead/ 24.5 24.5 7.9 7.1 8.25 7.84 313 64 87 Control (24.3-24.6) (24.3-24.9) (7.8-8.0) (6.4-7.7) (8.23-8.27) (7.54-8.10) (298-321) (63-65) (82-89)
Fathead/ 24.5 24.5 7.9 7.1 8.25 7.80 297 -
10.98% (24.3-24.8) (24.2-24.8) (7.8-8.1) (6.2-7.6) (8.23-8.27) (7.44-8.11) (287-305)
Fathead! 24.6 24.5 8.0 6.9 8.23 7.76 281 -
22 % (24.2-24.8) (24.3-24.9) (7.8-8.1) (6.3-7.6) (8.20-8.26) (7.53-8.02) (269-289)
Fathead/ 24.5 24.5 8.1 7.1 8.19 7.84 250 -
43.9 % (24.2-24.8) (24.3-24.9) (7.9-8.2) (6.5-8.0) (8.13-8.24) (7.62-8.04) (241-257)
Fathead/ 24.6 24.5 8.1 7.0 8.15 7.79 211 - -
72 % (24.2-25.0) (24.3-24.9) (7.9-8.2) (6.5-7.5) (8.06-8.21) (7.62-8.04) (203-216)
Fathead/ 24.6 24.5 8.2 7.2 8.08 7.80 170 *58 75 <0.10 100 % (24.3-25.1) (24.2-24.9) (8.0-8.4) (6.5-7.6) (7.93-8.18) (7.65-8.04) (165-175) (57-59) (72-76) (<0.10-<0.10)
Fathead/ 24.6 24.5 8.3 7.1 8.11 7.91 171 60 74 <0.10 Intake (24.4-25.1) (24.2-24.9) (8.1-8.4) (6.6-7.7) (7.99-8.18) (7.67-8.22) (165-173) (58-61) (72-76) (<0.1 0-<0. 10)
Daphnid/ 24.4 24.6 7.9 8.1 8.25 8.27 313 64 87 Control (24.2-24.7) (24.4-24.9) (7.8-8.0) (7.9-8.4) (8.23-8.27) (8.18-8.38) (298-321) (63-65) (82-89)
Daphnid/ 24.6 24.6 7.9 8.0 8.25 8.27 297 10.98 % (24.3-25.0) (24.3-25.1) (7.8-8.1) (7.9-8.3) (8.23-8.27) (8.19-8.39) (287-305)
Daphnid/ 24.7 24.7 8.0 8.0 8.23 8.27 281 22 % (24.3-25.1) (24.3-25.1) (7.8-8.1) (7.9-8.3) (8.20-8.26) (8.18-8.38) (269-289)
Daphnid/ 24.7 24.7 8.1 8.1 8.19 8.26 250 43.9% (24.4-25.1) (24.4-25.1) (7.9-8.2) (7.9-8.4) (8.13-8.24) (8.18-8.37) (241-257)
Daphnid/ 24.8 24.7 8.1 8.1 8.15 8.30 211 - -
72% (24.4-25.3) (24.4-25.0) (7.9-8.2) (7.9-8.3) (8.06-8.21) (8.18-8.42) (203-216)
Daphnid/ 24.8 24.6 8.2 8.2 8.08 8.33 170 58 75 <0.10 100 % (24.3-25.4) (24.4-25.1) (8.0-8.4) (8.0-8.4) (7.93-8.18) (8.18-8.50) (165-175) (57-59) (72-76) (<0.10-<0.10)
Daphnid/ 24.6 24.6 8.3 8.2 8.11 8.27 171 60 74 <0.10 Intake (24.3-24.8) (24.4-25.1) (8.1-8.4) (7.8-8.6) (7.99-8.18) (8.15-8.54) (165-173) (58-61) (72-76) (<0.10-<0.10)
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas Test, Sequoyah Nuclear Plant (SQN),
UV Treated Outfall 101 and Intake Samples, May 7-14, 2002 Temperature Dissolved Oxygen _H_ Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Chlorine Sample ID (OC) (OC) (mg/L) (mg/L) S.U. S.U. (irmhos) mg/L CaC0 3 mg/L (mg/L)
CaCO 3 Fathead/ 24.5 24.5 8.0 7.2 8.23 7.87 307 64 87 Control (24.3-24.7) (24.3-24.8) (7.9-8.2) (6.5-7.8) (8.18-8.27) (7.49-8.12) (296-315) (63-65) (82-89)
Fathead/ 24.6 24.5 8.0 7.1 8.22 7.87 292 - -
10.98 % (24.3-24.8) (24.2-24.8) (7.9-8.2) (6.1-7.8) (8.17-8.28) (7.48-8.12) (287-300)
Fathead! 24.6 24.5 7.9 7.2 8.20 7.89 277 - -
22 % (24.3-25.0) (24.2-24.8) (7.7-8.1) (6.5-7.8) (8.16-8.25) (7.45-8.10) (270-283)
Fathead/ 24.6 24.5 7.9 7.2 8.18 7.83 247 - -
43.9 % (24.3-25.1) (24.2-24.8) (7.7-8.2) (6.4-7.7) (8.12-8.24) (7.43-8.10) (240-253)
Fathead/ 24.6 24.5 8.0 7.0 8.14 7.83 209 - -
72 % (24.3-25.2) (24.2-24.8) (7.8-8.1) (6.0-7.7) (8.08-8.20) (7.42-8.08) (204-214)
Fathead/ 24.7 24.5 8.0 7.2 8.10 7.82 168 58 75 <0.10 100 % (24.2-25.3) (24.3-24.8) (7.9-8.1) (6.4-7.7) (7.99-8.17) (7.44-8.08) (164-173) (57-59) (72-76) (<0.10-<0.10)
Fathead/ 24.6 24.5 8.0 7.1 8.10 7.84 168 60 74 <0.10 Intake (24.3-25.0) (24.2-24.8) (7.9-8.1) (6.2-7.7) (8.02-8.14) (7.47-8.10) (165-172) (58-61) (72-76) (<0.10-<0.10)
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of -1 1I 1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building Bus Client SUPS P.O, Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN I
!1 I
Samule Custodyv- Fill In From Tnn f-lnun Relinquished By (Signature): Date/Time Received By (Signature): Date/Time ftA4 bed4.&'e----- - sAA- is'° FeŽ ----
ac°""z,
--___ tt*
r ... . . _ __ -)
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for eael sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of -1 I V Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building SUPS Bus Client P.O. Number: P0 BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN Container............
Field Identification I Grab/ Ship. ColcinDt/ie Number & Flow Rain Event?
Sample Description Comp. Temp. ( Volume (MGD) (Mark as Appropriate) . U.e
°C) Collected S .. .ppear .............. S .........
. ...... Start End Y- Iff.Yes,.No.Trace Yes, No Trace ESTT.Lag A..al......T.me ........... ..
....... Inches A 5/08/02 A SQN-101-TOX comp 4 5/7/02 //"s 2(2.5g) no 0i C cJ\ iNT~4it am...90 SQN-INT-TOX comp 4 5/7/02 5/08/02 1 (2.5g) no i ii0ii:i i i I2. e s
- ::: . ....::-'S..........
"< i i!*' : :*i
... ...... : : :: ::: :: :::: :: : i
- ;l jl t :
Sample Custody - Fill In From Top Down Relinquished By (Signature): Date/Time Received By (Signature): Date/Time Fee -0x Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4°C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page I of -1 U
Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building H UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_________
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN C ontainer :........:...: .. ...
Field Identification I Grab! Ship. Cleto aeTm Number & Flow Rain Event?
Sample Description Comp. Temp.
0 (Volume (MGD) (Mark as Appropriate) .......................
_______________ C) _______ Collected ___..._ ....
.°. C*)*C*o*lStart End ... .... o..::::i:::::: :.1nva1 :.I ::4.:i.y a:
SQN-101-TOX comp 4 5/9/02 2(2.5g) i*.. 7-j ........
5/ 0 0 ........... ......
.... . ............... :SA W LC:
SQN-INT-TOX comp 4 T!579/02 5/10/02 (2,5iI) i..I...
______________________________________Sample Custody - Fill In From Top Down__________________
Relinquished By (Signature): Date/Time Received By (Signature): Date/Time d_---' o ----oz s --- s .. ..q-..-..
Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them in ice. The hold time for eacl sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.
' It
Environmental Testing Solutions, LLC EnvionmetalTestng SlutonsLLCPage 33 Sample Receipt Log Date Time Received Received Sample Project Sample Received Received By From Temperature Number Number Sample Name and Description State Comments 04_2502__ J KXKeeJAA rebeY- '3.1 0C, .3ZS 2OL4ZS. 0 " 11'xPrAIJ. W?- k N-?5EI(J00 eye&AO Fete$X 3.IC S5Z3 020425 + dTbeAJ~i A03 1010 ~ sio 523 O2LfZ~0~QoST"cAI4 OuTFtALL OOZ 01 N.Z-s O Iwo0 KIEhee4MJ rebeg £AC-e~ 321307s.~ SS~FALL CX3 MO___
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~-ooz t~lKE~eeJAI'J (ilt)k4Ot/AJb 0 1 *C 321 02oSol. 03 tJAAVJeSV1ULOes~r"p~
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Environmental Testing Solutions, LLC _
Sample Receipt Log Date Time Received Received Sample Project Sample Received Received By From Temperature Number Number Sample Name and Description State Comments
ýrcAiol. 110Z KEkeaJA tJ rebe- .( Fi-C. 336Q 020S~ -01 ALCOA &CAee4e PObUCTS iJc~
~d~Z ~TVA SeQ UoIAH Nue-L. M~'
3 ooMd AJ rAge.
35~ 2 A. V6 I.(sC 335 3Zc),I0o3 &AKTrJt I-fe,44n-kAle Ggee At~
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on's O's&R0 qK§e; S Jjqff K6TKe&NAV Wem- 0. *C. 32 G oW 9" ~/G AIO,
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Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: T/VA Facility: Q* 31NAA tJc.*
C o rm ber:
MD bia tch : mS I ¢T '
D iluti on p be ter n d%o_
Or aue tvolum s (m e L): , Z A a n :
Diluent volume (mL) j r,' 17 2 Test organisminformation:
Test information:
Organism age: 2r-N-inutm OL-1b Randomizingtepae LIa' Twere born between: A)s q.o00 k-s-v 'r Inc uba to r nu mbe r :
s 0a -a*06 71Q30
. tc* - iuor
[OOrganism mes o rganis ms D at e and tisource: &-A C*06-0"0L OSL Artemia lot number: ft-W,02 P Transfer bowl information: pH Temperature Oven temperature:
-11,AR Z. 0" C- Drying time: - .atno6 Dailyfeeding and renewal information:
Controlinformation: Acceptance criteria 'I Summary of test endpoints:
%Mortality: . *20%
Average weight pr larvae: 7-day LC50 'J 5'
- 6. c/03 > 0.25 mg/larvae NOEC I*
I NOEC nr7. =
LOEC ChV I>00%
IC25
]
1C25
"&I A, UVA (
Species: Pimephalespromelas Client: -'JA-r S&Jct.A Date: o*--'1 -0"7.
N~o.ýýAe~b Survival and Growth Data Day CONTROL I0,q8%. - %
A B C D E F G H I J K L 0 /0 10 /0 /0 /o /0 /* /6 /0 10 /0 /0
/0 /0 /6 /6 /0 /0 /o /0 /0 /0 16 /0 2
//0 /D I/ 10 /og' / /0 /0/0 Z4 110 1 /0 /0 10 1/ /0 ' t 0 to 10 10 (C) 1010 /0(10 £~to 10 /0/0 6 10 to 1O 10 10 io 10 /o 'O /1 C to 7 o I q 4
/0/0
/ /0 A 1 0 /0 A= Pan weight (mg) wu
,..Pan+*Larvae weight(*mg) '. .v%* ,.- -10 . .
Larvae weight (mg)= A-B 1 4~2.
- k . qt*
- - - -J 'k.- - 60 Calculations and data reviewed:
Comments:
Y 0
0 2
CX
Species: Pimephalespromelas Client: -TV'Pr- S*_axl AA Date: 501-0 02.
Survival and Growth Data Day i %7I*Ae*
- Y Z AA BB 0 /0 10 /0 /0 I / D /0 JO , 0 2 /0 I1) 0O/0 3 10 b)10 t 0 4 /I/W(
16 /a- 10 6 o 10 6 6 10 2 &
A = Pan weight (mg) 14.d A -.
B = Pan + Larvae weight (mg) AD , , ,
Larvae weight (mg)=A-B t
__________(A._ 2S 0,55__ 4-vo Calculations and data reviewed .4-Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dates: May 7-14, 2002 Project number: 324 Reveiwed by: ),,_ _ _ _
Concentration Reeplcate Initial number of Final number of larvae A - Pan weight B - Pan + Larvae Larvae weight (rag) Wei l Wegh a /Intilamer11ý uvialMen (
eght (mmg coeffickat oof Pamct reduction from larvae (mg) weight(mg) - A- B of Larvae(mg) (%) variatio (%) casrtl ('h)
A 10 10 14.936 19.420 4.484 Control 0.4484 B 10 10 14.802 19.560 4.758 0.4758 97.5 0.4403 6.7 Not applicable C 10 10 14.888 18.940 4,052 D 10 0.4052 9 14.292 18.610 4.318 0.4318 _
E 10 10 15.039 20.660 5.621 10.98% 0.5621 F 10 8 14.904 18.510 3.606 0.3606 95.0 G 1 0 10 15.153 20.720 5.567 0.55679 0.4955 19.9 -12.5 H 1 10 10 14.676 19.700 5.024 0.5024 1 10 10 15.034 20.000 4.966 0.4966 22% J 10 10 14.831 19.460 4.629 0.4629 95.0 0.4461 14.2 -1.3 K 10 8 15.037 18.570 3.533 0.3533 L 10 10 14.684 19.400 4.716 0.4716 M 10 10 15.127 20.960 5.833 N 0.5833 10 9 14.679 19.160 4.481 0.4481 97.5 0.4974 16.9 -13.0 0 10 10 15.090 19.150 4.060 0.4060 P 10 10 14.587 20.110 5.523 0.5523 Q 10 10 15.134 19.340 4.206 0.4206 72% R 10 10 15.436 20.300 S 10 4.864 0.4864 97.5 0.4832 10 15.091 20.280 5.189 0.5189 9.1 -9.7 T 10 9 14.971 20.040 5.069 0.5069 U 9 9 14.788 19.700 4.912 0.5458 100% V 10 10 15.019 21.260 6.241 0.6241 W 10 10 14.791 19.710 4.919 0.4919 97.5 0.5494 10.0 -24.8 X 10 9 15.091 20.450 5.359 0.5359 Y 10 10 14.621 19.400 4.779 0.4779 100% Intake Z 10 7 14.681 18.570 3.889 0.3889 67.5 0.3639 45.0 17.4 AA 1 0 2 15.007 16.260 1.253 0.1253
_ BB 1 0 8 14.857 19.490 4.633 0.4633 _ ,_
Outal101 MSD = Minimum Significant Difference Dunnett's MSD value: 0.1116 PMSD Percent Minimun Significant Difference PMSD: 25.3 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant differece occurs for Environmental Intake Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7P from the control (detennined Dunnett's MSD value: 0.1617 Lower PMSD bound determined by USEPA (10th pereentile) = 9.4%/
PMSD: 36.7 Upper PMSD bound determined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, Off.
Environmental Testing Solutions, LLC Statistical Analyses Laval MhGratb and Surviv Tat-7 Day QGmh S-tar 5,V7102 Tat 11:. PpFRCR Sample a). TVA. Seqo -. Noeseod End Dam 5114102 La ID:. ErS-Ewv. TUVaSSolua SampleType: DMR-DIkd- Monimei; Report SampleD"M Pronoad EPAF 91-EPA Frhwsar Commens..P-i~hsm*m~ Ta: Species: pp-piql p-d Cone-% 1 2 3 4 D-Contol 0.4484 0.4758 0.4052 0.4318 10.98 0.5621 0.3606 0.5567 0.5024 22 0.4966 0.4629 0.3533 0.4716 43.9 0.5833 0.4481 0.4060 0.5523 72 0.4206 0.4864 0.5189 O.,069 100 0.5458 0.6241 0.4919 0.5359 Transform: Untramsformed Cooc-% Mean N-Mean 1-Tailed Isoak Mean Mn Max CV% N D-Coorol 1.-Stat Critcal MSD Mean N-Msar 0.4403 1.000 0.4403 0.4052 0.4758 6.725 4 10.9a 0.4955 1.1253 0.4853 1.0000 0.4955 0.3606 0.5621 18.944 4 -1.191 2.410 0.1116 0.4833 1.0000 22 0.4461 1.0132 0.4461 0.3533 0.4966 14.233 4 -0.12M 2.410 43.9 0.4974 0.116 0.4853 1.0000 1.1297 0.4974 0.4060 0.5833 16.891 4 -1.234 2.410 0.1116 0.4853 72 0.4632 1.0974 0.4832 L.O00 0.4206 0.5189 9.072 4 -0.927 100 0.5494 2.410 0,1116 0.4853 1.0000 1.2478 0.5494 0.4919 0.6241 10.014 4 -2.357 2.410 0.1A16 0.4853 1.0000 Axillazy Tests Statistic Critical Skew Shapie-Wiik's Tes adian normaJdiseibceoe (p> 0.01) Kert 0 9559744 0.884 -0.639853048 Bsgleas Teai dicate equalv.nar (p - 0.52) -0.108351025 4.198050499 15.08631706 Hypothesis Teat (1-tail 0.05) NOEC LOEC ChV TU MSDu MSDp MSB Dunmnts Test MSE F-4Prob df 100 >100 I 0.111504903 0.253429259 0.006341912 0.004287526 0.245395169 5,10 Ueeear Interpolalon (SORessmples)
Point % SD 95% CL(Exp) Skew IC05 >100
[CIO >100 ICI5 >100 1.0 IC20 >100 0.9 0: >100 1C40 >100 0.0 IC50 0.7
>100 0.6 0.5 o 0.4 C *'0.3.
02 e 0.1 0.0
.1 . ...... .
-0.2
-0.3
-0.4 0 20 40 60 80 100 120 Dose %
Dose-ResponsePlot 0.7 0.6 0.5 S0.4 0.3.......................................................1-tail.
- 0. 0.05 level of significance I,
sqnOS-07-O2data.xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 5/7/02 Test ID: PpFRCR Sample ID: TVA, Sequoyah - Nontuated Intake End Date: 5/14/02 Lab 1D: ETS-Env. Testing Solutions Sample Type: DMR.-Discharge Monitoring Report Sample Date: Protocol: EPAF 9 1-EPA Freshwater Test Species: PP-Pimephales promeas Coro*smes:
Cone-% 1 2 3 4 D-Control 1.0000 1.0000 1.0000 0.9000 100 1.0000 0.7000 0.2000 0.8000 Transform: Arcsin Square Root 1-Tailed Conc-% Mean N-Mean Mean MmN Max CV% N t-Stat Critical mSD D-Contol 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4 100 0.6750 0.6923 0.9935 0.4636 1.4120 39.790 4 1.872 1.943 0.3922 Auxiliary Tests Statistic Critical Skew Knrt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.880755126 0.749 -0.780447334 2.82895682 F-Test indicates equal variances (p = 0.03) 23.53577423 47.46834564 Hypothesis Test (l-tall, 0.05)
Homoscedastic tTest indicates no significant differences sqnOS-07-O2data~xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Stan Date: 5/7/02 Test ED: PpFRCR Sample ED:
End Date: 5/14/02 Lab ID: TVA, Sequoyah - Nonovated intake ETS-Env. Testing Solutions Sample Type:
Sample Date: Protocol: DMR-Dischargc Monitoring Report EPAF 91-EPA Freshwater Test Species:
PP-Pimephales promelas Comments:
Conc-% 1 2 3 4 D-Control 0.4484 0.4758 0.4052 0.4318 100 0.4779 0.3889 0.1253 0.4633 Transform: Untranslformed Conc-% Mean N-Mean I-Tailed Mean Min Max CV% N D-Concrol 0.4403 t-Stat Critical MSD 1.0000 0.4403 0.4052 0.4758 6.725 4 100 0.3639 0.8264 0.3639 0.1253 0.4779 45.002 4 0.919 1.943 0.1617 Auxiliary Tests Statistic Critical Skew Shapiro-Wilk's Test indicates normal distribution (p > 0.01) Kurt 0.842814147 0.749 -1,634364624 F-Test indicates equal variances (p = 0.02) 3.644213996 30.58085251 47.46834564 Hypothesis Test (I-tail, 0.05)
Homoscedastic t Test indicates no significant differences sqnO5-07-O2data.xfs
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1002.0)
Species: Ceriodaphniadubia Date Time Analyst Client: VA Se *oa*
Test start os.o--'oI z m4 I 10 Facility: ___ ________
Test end OS-tq02, IIsw l NPDES #: "V4- oQ M*sa Dilutionpreparationin ormation: Comments:
Diuton prep (%)
Effiuenvol.ume(mL)
Diluentvolume(mL)
Itom~ I ~-7-~1oa IL-00 t&.5 11-0 twk It 1ql.5!
q q.o
,0 o
I Test organism information: Test information:
Organism age: -<2A-- t'C046 OLU Randomizing template: AMJ-t.
Date and times organisms 1-%% -t O 'Tat- Incubator number:
Y C T batch: A SS , t.- Ota-7 were born between: 0q- U .03 2 Me6* I Organi sm source:
Transfer bowl information: pH = t. 25 Temperature= z4.1 Selenastrumbatch: AM 1-16I-02.
CONTROL Survival and ReproductionData number 7 8 9 10 1 2 3 4 Replicate 5 6 Day, 1 I Young produced C: 0 0 0
..... . t;[ 9 10.
Adult mortalty ..
2 Young produced OO r r)
I-, _..." ..
Adult mortality
[Ad u ungmp roduced [ '4 H C Adult mortality C . _
4 Young produced Adult mortality 0
I5 IQ ' i 7 -IA 5 Young produced ID o 10 1 Adult mortality I - '- 1-1 L-V'I -L .2 L ~
IZ L.~
1 . L..
6 Young produced Adult mortality j.I P P I 1 73 Young produced C) 0, I ' C 0 -0 0 C)
_ 7--* .
Total young produced 2s e ._ 2-q z,* 2-5 2 2 31 Final Adult Mortality X for 3` Broods >< '-
Calculations and data reviewed:
Test Renewal, Feeding, and Incubator Location Information Mon Tue Wed Thu Fri Sat Sun Date 054 Time m I' l M 2 16__ L=
Analyst Shelf __. 1 .62.
Location Control information: Acceptance criteria Summary of test endpoints:
% of Male Adults: :520% 7-day LC50 >)M
% Adults having 3 rd Broods: I02'. >80% NOEC IVr.
%Mortality: M < 20% LOEC "-100%
Mean Offspring/Female: 2".6 > 15.0 offspring/female ChV -*
%CV: go/'* <40.0% IC25 >10M____
Species: Ceriodaphniadubia Date: (s-o1-oZ..
Client: TVI,- SeeQ>%ŽNA CONCENTRATION: W).A9%
Replcate number Da y o ro6 e 3 7 9 10 Adult mortality C) C) ~ 0 2 Young producedC C 0 Adult mortality t [ ...- __ . U 3 Young produced a 4 [
Adult mortality L. - .- T U 4oYoung produced qra Adult mortality t 5 Young produced 10 _I?
Adu itmortality JL U-. I L-j XL n k-4 k6 Young produced 1ja9j{Ij lT0 i~
Adult mortality L Red utl : .
7 Young produced I Total 2 ) n8 I Z 29 'I 405a 3I I- zC) 2__
aon aroaucea ia1 a~ 2-a3a '
Final Adult Mo rtali ty Su.viv=al an Concentration: Dt A r% Mortality: m0yI Mean Offspring/Fexnale: eo
% Reduction from Control: *3 .
Calculations and data reviewed:
CONCENTRATION: Survival andReproduction Data Day ________________ 2 3 ~~~ Replicate nnumber ___ ___ ______
Day 1_____3_4 5 1 6 7 8 9 10 Adult mo__a l__y AdAutmortality L .. .U. i 2 I0 g2 Young produced
- 0 C C 0 0O 2_talYoung produced cedk[1 , _ _
Young produced o q 5Ic VF7 3TL-1 oW
___ Adult mortality ~
_ _~ _._l_ Il ? t-. __T JT j~
5 A Young t mortality FindAdul produced Adult mortality I
I_
LLIi.J U T10 L- 7 7 T 1
__ L T~
LL- K 4s" P 1:7- t.
6 Young produced IsI 9 q '0 1 E
_ Adult mortality [LI -_~tI~I L-FN 6 Young produced 0~~ -0 1-1 Z5 Total young produced ;?o-9 at.0Z725 P Final Adult Mortality_ tZo CT L- L,17-L t 1L Concentration:
%Mortality: 0070 Mean Offspring/Female: 2k.,5
% Reduction from Control: "3. 3.
Calculations and-data reviewed:
Species: Ceriodaphniadubia Client: -TVA - See,-q,. 1AA Date: oS- ot1 -o-4.
CONCENTRATION: 4_V Surivl ndRe~odctonData Da Da __
_ ___ 1 2 :3 4 5 Replicte number 6 7 8 9 10 1 Yo u png pr du e o I C) o Z 0 6 (> 63 6*
2 Young produced 0" 0 0 0- ( Q Adult mortality %, t--I LT %-, t.
"2 Young produced AI *4 C) -' T 4 _onpou__U 0 c) 5 Young d produced r_"._ 1
-inal Adult mortality .-. L I.- T L 6 Youngoproduced )An Ise IS Aultmortality M rtly L 1 1 5 Young produced Opg1A, 03 l 6oa Young produced .L Adut L.toralty.~% Reucio fro Conrol-*--,
Fn Adult mortality L K _- 7 -. L..
-Concentration:
%Mortality:
Mean Offspring/Female: 25i, 0
%Reduction from Control: -,S,%%
Calculations and data reviewed:
CONCENTRATION: I %Survival and Reproduction Data I ,,,-mr -- I .. I I _____
.- I L-Replicate number ____ ___
.-- m.
Day_ _ 1 1 2 1 3 1 4 5 6 7 8 9 10 Total young produced r - T -- 2. -*0 " . ='-
Autmortality - t1_ -
Final Adult 2 Young Mortalitypro~duced [ T? C)_. C_ ? *?
- 010 *-t *.Z
- C _.
3 Young produced 1_ 10-4I)
\__3 1.. 0QC Adult mortality _____i__ ___ __ __
__ Adultimortality L _ __ __ L 5
_IAdultYoung produced mortality I#
I L_L4 7_1z l_ 1 LU I _I 10 L_
/0~& 10 L_
=
L-1?-
t-6 7
Yung produced Adul mortality Young produced 6 C
1____
__1_ 1 0
.I L
1__
I 4 '7 1 ICA.I I 11 Total young produced Z- 7-6 Zce 0 2-1 _31 Z1 I -1 :30 3Z 3'-1 Final Adult Mortality jTT7(\ - ~ ~~
Concentration:
I% Mortality: 00f.
-Mean Offspring/Female: Zq, I
%Reduction from Control:
Calculations and data reviewed:
Species: Ceriodaphniadubia Client: TVA - Sea-A* k Date: OS-0"-...
CONCENTRATION: Jb'01 Survival and Rflhz" Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced _ . ._01 . 0. 0 0 0 2 Young produced [ '4 O - I O - - -
AYoung produced C_)--- - = I - -
Adult mortality ,U. . .
-- ,m,, " -- I
- I1 0 ',-.
Young Y produced
_______ L L.1L 07> o 0o 10J _
. A Adult mortality 1 ' 1 - - * - - t 5 Youngoproduced C Aent Adult mortality ai t 7nYoung produced -C- O-7 fj:4 Total young produced ~ ~ '& 3 ~ 3 S 3 L Final Adult Mortality -T 17 .
Concentration:
% Reduction from Control: - tZ.0.*/
Calcutlations and data reviewed:_.
CONCENTRATION: I C).* Survival and ReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 10 1 Young produced 0 6 0C) 0I I - ý)
iAdult morat L-7 - L Adult mortality _ ._, _
3 [Young produced _
0 I I0] 01 I 7XI3A4 0
[Adult mortali'ty 1C7 1 j]k I 1 ~
4 Young produced " t.4 5 -5 C F Adult mortaltyL __ C_ _ -
5 Young produced __
10__1 6
_IAdult 7
Young produced mortality]
Young produced 1IC)1
..A
(-I IL L.,~ .. V 0. 10 0 0i~ 0 t.-L_]LL-4L 17-K? i+/-7 t4Q 0
Toalyongprdued2Jz-Zj 15 1'4 123 le~ 240a Final Adult Mortality j ~ jU]T ~ --
I%Mortality:
Concentration:
Mean Offspring/Female: ZZ.,0 I
% Reduction from Control: ZO.%
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dates: May 7-14, 2002 Project number: 324 Revelwed by:
Concentration Replicate number Survival Average reproduction Coemdent of Percent reduction from
(%) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (%) control (%)
Control 25 28 31 32 24 26 25 25 29 31 100 27.6 10.8 Not applicable 10.98% 26 28 28 29 29 31 27 26 32 30 100 28.6 7.0 -3.6 22% 26 31 26 32 29 31 30 27 25 28 100 2&5 8.6 -3.3 43.9% 25 28 26 31 29 34 28 28 30 31 100 29.0 9.1 -5.1 72% 27 26 30 27 31 27 27 30 32 34 100 29.1 9.2 -5.4 100% 30 31 28 30 28 29 33 35 33 32 100 30.9 7.5 -12.0 100% Intake 30 22 24 21 15 14 23 18 26 27 100 22.0 23.5 20.3 Outfail 101 MSD = Minimum Significant Difference Dunnett's MSD value: 2.593 PMSD = Percent Minimum Significant Difference PMSD: 9.4 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests Intake: when a toxicant reduces Ceriodaphnia reproduction by 12.6% from the control.
Dunnett's MSD value: 3.272 Lower PMSD bound determined by USEPA (I 0Oh percentile) = 11%.
PMSD: 11.9 Upper PMSD bound determined by USEPA ( 9 0dt percentile) = 37%.
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceo hnia reproduction in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Environmental Testing Solutions, LLC Statistical Analyses Cerodaphnia Survival and Reproduction Test-Reproduction Start Dae:. 5f7/02 Test ID: CdFRCR Sample ID: TVA, Sequoyah - Nowreated End Date: 5114/02 Lab ID: ETS-Env. Testing Solutions Sample Type: DMR-Discleargo M toring Report Sample Daze: Protocol: EPAF 91 -EPA Freshwater Test Species: CD-C a dubia Comment:
Conc-% 1 2 3 4 5 6 7 3 9 10 D-Coonol 25.000 28.000 31.000 32.000 24.000 26.000716X25.000 25.000 29.000 31.000 10.98 26.000 28.000 28.000 29.000 29.000 31.000 27.000 26.000 32.000 30.000 22 26.000 31.000 26.000 32.000 29.000 31.000 30.000 27.000 25.000 28.000 43.9 25.000 28.000 26.000 31.000 29.000 34.000 28.000 28.000 30.000 31.000 72 27.000 26.000 30.000 27.000 31.000 27.000 27.000 30.000 32.000 34.000 100 30.000 31.000 28.000 30.000 28.000 29.000 33.000 35.000 33.000 32.000 Tranform: Untransformed l-Taled Conc-% hotoiak Mean N-Moan Mean Min Max CV% N t-Stat Critical MSD Mean N-MeaM D-Contol 27.600 1.0000 27.600 24.000 32.000 10.829 10 28.950 1.0000 10.98 28.600 1.0362 28.600 26.000 32.000 7.032 10 -0.882 2.287 2.593 28.950 1.0000 22 28.500 1.0326 28.500 25.000 32.000 8.634 10 -0.794 2.287 2.593 28.950 3.0000 43.9 29.000 1.0507 29.000 25.000 34.000 9.051 10 -1.235 2-287 2.593 28.950 1.0000 72 29.100 1.0543 29.100 26.000 34.000 9.228 10 -1.323 2.287 2.593 28.950 1.0000 100 30.900 1.1196 30.900 28.000 35.000 7.544 10 -2.911 2.287 2.593 28.950 1.0000 Auxiliary Tests Statistic Critical Skew Kurt Kolnogorov D Test indicates normal distrbution (p > 0.0 I) 0.83713907 1.035 0.298146516 -0.9893687 Bar.let's Test indicates equal variances (p 0.9 1) 1.532001376 15.08631706 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dumnett's Test 100 >100 1 2.592676189 0.093937543 11.95 6.427777778 0.136908096 5,54 Linear Interpolation (80 Resamples)
Point % SD 95% CL Skew IC05 >100 ICI0 >100 ICI5 >100 1.0 1C20 >100 IC25 >100 0.8 IC40 >100 0.8 IC50 >100 0.7 0.6 0.5 0.4 00.3 0.2 0.1 oo .1 e_. *..
0.0 . * ....... _... .. .
-0.1 .... - .
-0.2 0 20 40 60 80 100 120 Dose %
Dose-Response Plot 40 35 o25- - - - - - - - - - - - - - - - - - - - --.. 1-tail, 0.05 level
.2 2of significance 20
- 15 10 5
0 CD os o;
05 C-1 sqnO5-07-O2data.xls
Environmental Testing Solutions, LLC Statistical Analyses Ceriodaphnla Survival and Reproduction Test-Reproduc*_on Start Date: 517/02 Test ID: CdFRCR Sample 0D: TVA, Sequoyah - Nontrea*ed .
End Dam. 5/14/02 Lab UD: ETS-Env. Testing Solutions Sample Type: DMR-DIucharge Monitoring Report Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: CD-Ceiodaphnaa dubia Comments:
Conc-% I 2 3 4 5 6 7 $ 9 10 D-Coitrol 25.000 28.000 31.000 32.000 24.000 26.000 25.000 25.000 29.000 31.000 100 30.000 22.000 24.000 21.000 15.000 14.000 23.000 18.000 26.000 27.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 27.600 1.0000 27.600 24.000 32.000 10.829 10 1100 22.000 0.7971 22.000 14.000 30.000 23.473 10 2.968 1.734 3.272 Audllary Tests Statistic Critical Skew Kurt Shapiro-Wilkls Test indicates normal distribution (p > 0.01) 0.983128428 0.868 -0.124038982 -0.311539277 F-Test indicates equal variances (p = 0.12) 2.98507452 6.54108572 Hypothesis Test (I-taiL 0.05)
Homoscedastic t Test indicates significant differences sqnO5-07-O2dataxLs
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: "rV A Facility: -F-MAiA M* *4 r" NPDES #: TN- O*O*24,S) o9-* A-Teb Dilution preparationinformation: Comments:
MHS batch: O 0-oz- & It" Dilution prcp(I%)t -z7- c46.41 -1z on~ 2 ýtu_&.e Oý .
Effluent volune (miL) ~ J. Ic Test organism information: Test information:
Organism age: .q-AoUeL. 0OLb Randomizing template: 'IeUzou)
Date and times organisms CS-06-O2 IoW -To 1400 M.ST7- Incubator number:
were born between: t 9.I
- t koo C-c Organism source: A& ,6ATcA os- o6-62. Artemia lot number: &*'oz01f Transfer bowl information: pH = Temperature = Oven temperature: (,w
"".___2__, 0"*C Drying time: Z4.
Dailyfeeding andrenewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time o0ce--Oz - $- 00,oo, I C6 aCZ
___ ~q 1 )(all 15/-3 2 oG-o0 -a /000 1&o2. /&0 Z 3__ 05_____0 OqS,3 IL04 Nzz.
6 OG I____- 1HO t*, .4 4q-6 7 1__
- -oz- ____.__
Controlinformation: Acceptance criteria Summary of test endpoints:
%Mortality: I 2. -s°7. :5 20% 7-day LC50
- Average weight per larvae: O.q"-1 >0.25 mo/larvae NOEC LOEC IC25
Species: Pimephalespromelas Client: hI" A- GeWMA Date: O-O1-O'7 Survival and Growth Data Day CONTROL -O. -Z*
A B C D E F G H I J K L 0 I /o 10 /0 10 /0- /610 160 S/0l 10 0/0 /6 /0 16 /0 /0 /01 /010 2 11/0/0/0/0 /0/0/ /0 / /0 3 /0 /C) /0 / I0I/0 o O 10!o 6 0 t'O 10 0 t /001 4 I/0 0 10
/D to /0 / 0 /0 10 10 /0 /0 /0 lb 7 f / e 6 IO /0 q 0 /6O 10 /0 A=P a ri I a 0 \
1111% 02 Larvae weight (mg) 0 -
B~ Pan +
Calculations and data reviewed: J r Comments:
Species: Pimephalespromelas Client: "T'* A - Sesoxg AK Date: 05- 01-o-.
uN- -T4qetr>
Survival and Growth Data Day 1,
-2%.7 -orff.
_____M N 0 P Q R S T U V IW X 0 0 /0/
10L /0 /0/0 /0 /0 /0/0 /0 1 /010 0 /0 010 I* /4 0 /0' /0 2 /0 /0 10 0 0/ /0 /0 /O /c /0 /0 3 10 '(0 '1 to lo t 011 I01 10 10 10 0Q 4 /0 I0Cb t 10 10 10 10 10 10 10 10 10 5_/a /0 1001c_ 10 10 tO 1o tO 0 1o 10 0 Q(
6 to to 10 Io to)o o /o 1 o io q10A 0 7 /0 /) 10A /0 /0 /101 l 16 10 A =Pan w eight ( mng) o v 46 V O M BPan + Larvae weight (rng) :70f4 o.b Larvae weight (ig) = A- B Calculations and data reviewed:
Comments:
Species: Pimephalespromelas Client: TVA-Seno'iAA4
%- Tje-Xe~tý-b Date: O's1c" -
I Survival and Growth Data Day iO*Ot, J 'AI(e Y Z AA BB 0 /0 10 /0 /0
/0 /0 /0//0 2 /0 /0 /0 /0 3 1O 0 Io
' JO (010 10 0 4j JO ic( ID 5 to 10 16 1C0 6 10 1(3 /0 Io 7 /0 Ito I /0 / 01 C2alculations and data reviewed:
Comments:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Test dates: May 7-14, 2002 Project number: 324 Revelwed by: A L
)'l Replicate Initial anrber of Finl nuolieroftlrvae A - Po weight B - Pan + Larvae Larvae weight (rma Weight / Initial nauber Mean survival Mean weight (WOg) CoPadct Of ar.tredactdiea ho Concentration (%)
larvae (rag) weight (ing) A- B of larvae (ag) (%) vartatim (%) comtrol(%)
A 10 10 14.632 19.570 4.938 0.4938 Control B 10 10 14.634 19.090 4.456 0.4456 97.5 0.4734 4.3 Not applicable C 10 9 14.701 19.450 4.749 0.4749 D 10 10 14.656 19.450 4.794 0.4794 _ __"_
E 10 9 15.020 18.790 3.770 0.3770 10.98% F 10 10 15.037 19.810 4.773 0.4773 95.0 0.4194 17.8 11.4 G 10 10 14.947 19.820 4.873 0.4873 If 10 9 14.870 18.230 3.360 0.3360 1 10 10 15.173 20.210 5.037 0.5037 22% J 10 10 15.004 19.840 4.836 0.4836 100.0 0.5092 4.7 -7.5 K 10 10 15.036 20.120 5.084 0.5084 11 10 10 14.791 20.200 5.409 0.5409 M 10 10 14.875 19.990 5.115 0.5115 43.9% N 10 10 15.060 20.220 5.160 0.5160 100.0 0.5333 10.2 -12.6 0 10 10 15.145 20.070 4.925 0.4925 P 10 10 15.008 21.140 6.132 0.6132 Q 10 10 15.362 19.940 4.578 0.4578 72% R 10 10 14.972 19.770 4.798 0.4798 100.0 0.4553 4.0 3.8 S 10 10 14.669 19.070 4.401 0.4401 T 10 10 14.787 19.220 4.433 0.4433 U 10 10 14.615 19.350 4.735 0.4735 100% V 10 10 14.759 19.560 4.801 0.4801 97.5 0.4734 8.7 0.0 W 10 9 14.933 19.130 4.197 0.4197 X 10 10 15.186 20.390 5.204 0.5204 ____
Y 10 10 15.015 19.780 4.765 0.4765 100% Intake Z 10 10 14.843 19.200 4.357 0.4357 100.0 0.4638 6.0 2.0 AA 10 10 14.982 19.450 4.468 0.4468 BB 10 I0 15.067 20.030 4.963 0.4963 _ I I I I MSD = Minimum Significant Difference Dunnelt's MSD value: 0.0747 PMSD - Percent Minimum Significant Difference PMSD: 15.8 PMSD is a measure of test precision. The PMSD is the minimum pereent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control (deternined through Dunnett's MSD value: 0.0333 Lower PMSD bound determined by USEPA (10th percentile) = 9.4/.
PMSD: 7.0 Upper PMSD bound determined by USEPA (90th pereentile) = 35%.
"The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection
Environmental Testing Solutions, LLC Statistical Analyses Lm-ral Fhh Growth and Survival Test-7 Day Growth Start Daue: 5/7/02 Test ID: PpFRCR Sample ID: TVA, Sequoyah - UV-,aated End Date: 5/14/02 Lab ID: ETS-Eav. Testing Solutions Sample Type. DMR-Diaharge Monitortg Repor Sample Date: Protocol: EPAF 9 I-EPA P water Test Species: PP-Pihephtles Prontlas Commets:
Conc"-% 1 2 3 4 D-Conrol 0.4938 0.4456 0.4749 0.4794 10.98 0.3770 0.4773 0.4873 0.3360 22 0.5037 0A836 0.5084 05409 43.9 0.5115 0.5160 0.4925 0.6132 72 0.4578 0.4798 0.4401 0.4433 100 0.4735 0.4801 0.4197 0.5204 Transform: Untransformed I-Tailed haotonk Conc-% Mean N-Mean Mean Min Max CV% N t-Star Critical MSD Mean N-Meu D-Control 0.4734 1.0000 0.4734 0.4456 0.4938 4.272 4 0.4838 1.0000 10.98 0.4194 0.8859 0.4 194 0.3360 0.4873 17.798 4 1.743 2.410 0.0747 0.4838 1.00o0 22 0.5092 1.0755 0.5092 0.4836 0.5409 4.663 4 -1.153 2.410 0.0747 0.4838 1.0000 43.9 0.5333 1.1265 0.5333 0.4925 0.6132 10.169 4 -1.932 2.410 0.0747 0.4838 1.0000 72 0.4553 0.9616 0.4553 0.4401 0.4798 3.973 4 0.586 2.410 0.0747 0.4643 0.9597 100 0.4734 1.0000 0.4734 0.4197 0.5204 8.741 4 0.000 2.410 0.0747 0.4643 0.9597 Auxillary Tests Statistic Critical Skew KUnrt Shapiro-Wilk's Test indicates normal disatibution (p > 0.01) 0.980244517 0.884 0.193884967 0.15776262 Bardtets Test indicates equal variatces (p 0. 14) 8.409856796 15.08631706 Hypothesis Test (I-tail, 0.08) NOEC LOEC ChV TU MSDu MSDp MSH MSE F-Prob df Dunno's Test 100 >100 1 0.074689285 0.157763711 0.00641525 0.001920934 0.026198762 5,18 Linear Interpolation (80 Resamples)
Point % SD 95% CLExp) Skew IC05 >100 ICIO >100 ICIS >100 1.0
[C20 >100 0.9 IC25 >100 0.8 IC40 >100 IC50 >100 0.7 0.6 0.5
& 0.4 S0.3 0.2 0.1 .
-0.1
-0.2 - -. -
,- 0.2 1.. . . . . . . . . . . . . . . . . . . .
0 20 40 60 80 100 120 Dose %
Dose-Response Plot 0.7 0.6 0.5 04 1-tail, 0.05 level
- - 04 of significance 0 Ct. N 0 Ct 0 0
Sr D
sqn_05-07-02data.xis
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/7102 Test ID: PpFRCR Sample ID: TVA, Sequoyah - UV-Treted Intake End Date: 5/14/02 Lab ID: ETS-Ean. Testing Solutions Sample Type: DMR-Dvis ge Monioring Report Sample Daze: Protocol: EPAF 9 I-EPA Freshwater Test Species: PP-Pinephales promelas Comments:
Conc-% 1 2 3 4 D-Control 0A938 0.4456 0.4749 0.4794 100 0.4765 0.4357 0.4468 0.4963 Trnstform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Mn Max CV% N t-Stat Critical MSD D-Coatrol 0.4734 1.0000 0.4734 0.4456 0.4938 4.272 4 100 0.4638 0.9797 0.4638 0.4357 0.4963 5.965 4 0.560 1.943 0.0333 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicateu normal distribution (p > 0.01) 0.93363601 0.749 -0.063500364 -1.353307 16 F-Test indicates equal variances (p = 0.62) 1.070973468 47.46834564 Hypothesis Test Il-tail, 0.05)
Homnoscedastc t Test indicates no significant differences sqnOs-07-O2data.xLs
Environmental Testing Solutions, LLC 1000.0 and 1002.0)
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Methods Species: Ceriodaphniadubia and Pimephalespromelas Summary of Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: May 07-14, 2002 Reviewed by:,
Project number: 324 Ceriodaphniadubia Pimephalespromelas 24.41 25.1 24.61 0.21 2. _43 24..6 0.
24.2 4.
24.6 0.3 0.3 24.5 0.2 24.4 251 24.21 L244.9 25.4 24.7 0.3 24.2 25.1 24.5 0.2 24.2 Overall Temperature (C)
(including all concentrations for initial and final temperatures)
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: May 07-14, 2002 Reviewed by(4 - ":'
Project number: 324 I
m I I Im N ----.. .. '-.. I Concentration Parameter Initial Final lmtiai Day 4 1 Day 5 1 1 Final I Initial I Final I Initial I Fna Day 6 I 91 8.27 7Initial Final7 7.901 Initial.271
- 8. 7.671 8.251 7.861 8.25 .,i
___________I.
pH (SU)
DO (mglL) 7.9 6 ?: .41 7.8 6.6 8.0 7.2 8.0 6.
Conductivity (jumhbo/cm) 321 308 89 Control Alkalinity (mg/L CaCO3) 63 Hardness (mg/L CaCO,)
244 243 246 246 24.5 24.51 246 24[31 249 24.4 24.5 24.3
_Temperature CC) 766 8.23 7.91 827 7 8.24 7.841 8.24 pH826 811 824 DO (mg7L) 7 8.0 8.0 10.98% Conductivity (pmhos/cm) 299 299 305 )2
__________697:28 22% Conductivity 0tmhos/cm)282 Temperature CC) 246 2432 24.6 24.6 24 62 24.5 24.5 24.2 24.4 (SU)804 823H 822 766 8.3 7 8.24 8.1 8.21 7.95 DO (mg7l)6 79 6 8.0 81 7 81 8. 7 8.2 22% Conductivity (pmhos/cm) 257 25 28 289 241 244 282
'TemperatureCC) 246 24.3 245 6 24.6 24.6 246 24.5 24.5 24.2 (SU)804 8H 82 2 8.06 7.86 82 810 8.0 7 8.1 7 DO (mg7L)5 79 8.0 7.4 81 82 6 8.2 8.2 724.% Conductivity (tmhos/cm) 212 21: 25_ 241 244 8.4 821 76 8.6.0 0 pH (SU)8.0 Temperature DO (mg/L)
CC) 24.8.1 24.3 75 79 24.5 6. 24.6 8 0 24.6 4 268.0 24. 3 258.8282 24 24.5 24.2 24.6 Conductivity (Jpmhos/cm) - ý 1M15MMI_
100% Alkalinity (mg/L CaCO 3) 57 Hardness (mg/L CAC03) __L]
Total Residual Chlorine (ra g/L) < .01 2 .1 2 .1 243 211 49 44 2 Temperature (CC)24r 2.1 2.1 2,1 2.1 2.J 247 7.99 7.96 8.1 8.10 7.67 8.05 7.98 8.16 8.2 (SU)7.99 8H 8.14 7.69 100% Intake C1 nducainity (pmhg /cm)
AlkaHlnty (m9/L C*CO3)
Hardnes (mg/L CaCO3) iTotal Resildual Chlorine (ragL) 60 7
U0<.0F<01 I0 56
[7
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphniadubia Daily Chemical Analyses
- nt: Sequoyah Nuclear Plant Non-Treated t dates: May 07-14, 2002 Reviewed by.
ject number: 324 Parameter Day 0 Day I Day 2 Day 3 Day 4 Day 5 Day 6 mcentration Inta inal I I nta IFil Iiil Final I niilIFnl Initial I Final !Initial Fial Intal Fina pH (SU) 8.27 8.26 8.23 8.38 8.24 8.26 8.27 8.26 8.27 8.18 8.25 8.28 8.25 8.30 DO (mg/L) 7.9 8.1 7.8 7.9 7.9 8.3 7.9 8.1 7.8 7.9 8.0 8.0 8.0 8.4 Conductivity (jtmhos/cm) 319 3142 298 308 Control Alkalinity (mg/L CaCO 3) 641 65 Hardness (mgfL CaCO3) E 89 82 Temperature (CC) 24.3 24.5 24.6 24.9 24.3 24.7 24.7 24.4 24.8 24.5 24.7 24.2 24.4 pH (SU) 8,26 8.20 8.24 8.39 8.23 8.25 8.27 8.2 8.24 8.19 8.24 8.30 8.24 DO (mg[L) 8.1 7.9 7.8 7.9 7.9 8.3 7.9 8.0 7.8 7.9 8.0 8.0 8.0 8.2 10.98% Conductivity (jmhos/cm) 30-1-'- 29-----0 2-- 28- 297 Temperature (*C) 24.6 24.3 24.7 25.0 24.6 24.7 24.74 24.4 25.0 25.1 24.6 24.6 24.3 pH (SU) 8.26 8.23 8.24 8.8 8.20 8.25 8.26 8.24 8.21 8.18 8.21 8.31 8.24 8.29 8.1 7.9 7.8 7.9 8.0 8.2 8.0 8.0 8.1 7.91 8.0 8.1 8.1 DO (mg/L) 22% Conductivity (pmhosIcm) 287 2828--5269 273 282 Temperature (CC) 24.8 24. P 24.7 25.1 24.6 24.7 24.8 24.4 25.1 25.1 24.6 24.6 24.3 24.4 pH (SU) 8.23 822 8.22 8.34 8.13 8.25 8.24 8.20 8.16 8.18 8.15 8.3 8.21 8.26 8.2 7.9 7.9 80 8.0 8.2 8.1 8.0 8.1 7.9 8.1 8.3 8.2 8.4 DO (mg/L)
Conductivity (pimhos/cm) 254 253 252 257 241 244 250 24.8; 24.5 24.6 25.1 24.7 24.7 24.7 24.4 25.1 25.0 24.6 24.6 24.4 Temperature (CC) 208 8I4 818 R8I3 S... ... X
]l,I R /] /*t /I /rt O,*1/ O.LV I O..*U Q In O.IU I Q11 U.A*D DO (mgIL) 8.21 7. J .9 80 . 8.3 8.1 8.210 8.2 8.31 8.2 8.3 72% w 216 ~ 203 206 ~ 2111 Conductivity (jumhoslcm) 212 213 213 Temnerature ("Cl 25.1 24.5 24.61 25.0 24.71 24.7 24.71 24.4 25.3 I 25.0 24.6 2. 24.41I 24.4
. . ._ C... I _ Y_ ^X. I ^ 1 t,-
pH (SU) 8 151I 82 jz 181 247 1.9.11 *.21
"- -" i 8.
DO (mg/L) .2 8.1 8.01 8.4 8.31 8.8 8.38 8.4
.5-Conductivity (jimhos/cm) 100% Alkalinity (mg/L CaCO3 )
I 8.158.14 8.35 7.991 8.267 .8 81 .0 81 .5 82 .6 85 Hardness (mg/L CaCO 3)
Total Residual Chlorine (mg/L) <0.11-0 <0.11 i<0.10
.11 245 47$ 48 2.1 2.71 249 2. 25.44. 24.36 Temperature (0C)
CC Tempratue I3 .11 814 83 7.991 821 .8 8.10 8. 05 8.2 8.161 85 PH (SU) - 8.1 8.1 83831.8.1 8.41 . . 8.2 .
DO (mglL)
_I - 8 .21 Conductivity (Jmhos/em) 100% Intake Alkalinity (mg/L CaCO3) G0 Hardness (mg/L CaCO 3) <
1 .
Total Residual Chlorine (mg/L) <
Tpmnnrahure N.1 24.71 24.51 24.61 24.8 24.31 24.7 24.61 24.41 _24.81 25.11 24.61 24.61 24.51 24.4
avironmental iesting 64)Auitiuoii, jL4t.
Species: Pimephalespromelas Client: T1VA SLOAQMAA~ Date: oe,.61 07 oATe Full-strengthChemistry: O),viAL ICA Parameter SampieNumber: .ControlBatch:
__ ___ __ 1 2 3 eso~-Z 05-0iO'-z pH IS" s&o
-Z-Ss1___
(S.U.) __ _ _ _ __ __ _
DO A, =
Conductivity I1.10Z AlkainitySA (mg CaCOJL) I__
S Hardness (ma CaCO-JL) .
?
Chlorine400 Collection start date 0-so S46 so-7 Collection end time 1350 112 G Grab or Composite -900e-~ -ýAO (duration) ICEOMo CM/e oMs Temperature CC) upon receipt 0. 5C .3'. p Physical AA I-e 'e characteristics MfliJ T-r7WA CUeAgL CW1A4. C~eokL Dates sample used 6667o %~o-41z 6Sl1-OI7 og-d~
-a O S ).100 W-M'IZ os-ts .oz ETS Project and 2S1-0 aze Sample numbers aO2O601.6O OtOS4I. OZ ~oIL.
Page 6 ot 8 Environmental Testing Solutions, LLC I Species: Pimephalespromelas Client: T-NA 'SkQi Avk Date: S-0"1-MZ.
I Full-strength Chemistry: -PCY.
Parameter Sample Number: Control Batch:
_j 1 2 3_--_1 pH Rz1 t.2_q (s.U.)
DO R-.Z 1.z nos 2.O~
(mg/L)
Conductivity 3ZI t,2T..
110 .31 (Vmhos/cm)
Alkalinity 10 LI (mg CaCO3IL)
Hardness T10 (mg CaCO3IL)
Chlorine 40,10 ,40.1O <0.10 (m9gL) I 1 T9 1 l Collection start date Collection end time 786140u-o 4 0q
~Iu*
Grab or Composite (duration)
Temperature (TC) 1,*'C upon receipt 05-011.01 Physical tVe -MO characteristics LC e.t*A T-AtJ CQLAA C.Le.Ase Dates sample used OS0z-0 a~-10 O-II-ot.
c.5V-at 02 ETS Project and OaWS0 43 o'WS1102 Sample numbers r .M--.ý
Environmental EnirnenalTstn Testing Solutions, Souios LLC LL ragc I ui 0 ae io Species: Pimephalespromelas Client: Tr1A iuAtHA Date: 05- dl Daily Chemistry:
Day Concentration Parameter 0 2 CONTROL pH (S.U.) 82 eI L DO (mg/) 3. ,/ =. oq 3q Conductivity (Amhoskcm) 3Z1 Temperature . . ; r a'LS ow2-15 DO (m g/,) .. 8. I L- -.w -O3 q .
Tepea0 r 2q'.5 al.4 zq 2(P It) 0 f; Conductivity ......
PH (S.U.) 6SZ.e ,1 18O2_
Temperature Zq(0 Zq. 2- 24 .6, pH (S.U.)
DO (rag/L) , "- . "- f, 8.1..
. "* 0.,
ZLZ, Conductivity Temperature
, G, 7q.
CC) 3 (Wnihos/cm) pH (S.U.) &-Z4 R.0 I 84Z I1, 0,1.3 "q
- j DO (rag/L) . .o . ,
- iq.5'4 o Conductivity Temperature 2q. 3 Z V. G Li.~o
"*Z (Wmhos/cm) pH (S.U.)
DO (mg/L) ,oq ".9 1.:4, Zl *," ,*. 1z
/ 3tv Conductivity (pmhos.cm)
Temperature zq. 2'k.
2 . . 4 '
zq.l G 24./(o pH (S.U.) ".
1-w** 8 0 _ *! *.3
]b6 % DO (mg!L)
Conductivity JVrKg Conductivity I*/?
- 17. m w, 1007., '(Wmhos/cm) / E M R3 I TS Temperature , 2 1 24.o 2', IA/4 'L(n CC)
Initial 11 Final I Initial 11 Final I Initial 11 Final I
I.
Species: Pimephalespromelas Date: U .071-o2 Client: -N F\ 001AA Se 5 6
.Concentratif 5~Z 6.(
Conductivity Temperature j4 II~cC)
PH (S-.U.)
DO (mgd/L) ZI-43 z-. (3fa Ozq Conductivity 24 J241Z3
( )mhS/CM) L~~z4.3 aLL Temperature NO~' Zq 0cC)
- a -
2413 z2-24 t4 q*
s 41
-241 Conductivity .1 9 i ognhs/cm)
Temperature
-I (00 PH (S.U.)
DO (mgfL)
Conductivity Z) hsCM)
Temperature 2qd z*3 q -*q.q 2q*5 2,q.
I 0cC)
PH (S.U.)
DO (mgLL)
Conductivity 2.0-3 211p
,1 (pxnhos/cm)
Temperature 24.(, 2~b 2(.3 2.'~ cI.S zq.z 24ý Conductivit 1:15 TemperatureS. 2 q.1 24 1?q. '.3 zl pHE (S.U.)_
DO (mngtL) 14wo% Conductivity (junlios/c Temperature 0cC) __
Iita Fnl I Final initial Final initial Fia Initial
Page. 01 8 Environmental Testing Solutions, LLC Species: Ceriodaphniadubia Client: -T-JA"- *rh- %AlA Date: O S- 01-O "-
Full-strengthChemistry: OX-VjAu- Ilo Parameter Sample Number: I Control Batch:
1 2 3 O[*a-aOiZ 05- 0 o2 o' -o\0.
pH -7.3 ý. 7,-1 (s.U.)
DO 8.2 .&-0 1 'e., 3 Conductivity '-'a 321 (gmhos/cm3 51"7 tpq Alkalinity (mg CaCO3/L) 82.
Hardness "lh. 12.
(mg CaCO 3/L) I40,10 Chlorine '(Q, IC .'osjo (mg/L) m I
m i a Collection start dat; .S-o01c7 or-Mq-oT.
Collection end time owbS-O1 Grab or Composite (duration) -mz~, i. fcc' Temperature (QC) o, 3'C o.5.C upon receipt Physical "1370 1q2 characteristics 1c1A
~CLeAA9 Dates sample used j og-01-ot 45o10-OZ,.1051'-2-07-.
a4-t3-oz ETS Project and 02o z A.0d,0--- ooS7I~ol Sample numbers 02ZcZd1.
2 Lf 1
Environmental 'esting Solutions, LLL Species: Ceriodaphniadubia o Date: US_-6"_-_"_-_
Client: VArJA - !&
Full-strengthChemistry: N-rA'e..
Control Batch:
Parammeter 3.
g.z1 Z:1 *.*.5 rpH- g 1"5 "l.411 *10 1.0
-7I.
112 1(05 94-I Conductivity 63" 1 ___ _
mhýos/cm)
Alkalinity 8q I ,*9 ,
Hardness q 7ý 12 Chlorine "0;0 -(0.10 40. 10
-Z ,01-oZ c6-,-07 Collection start date Collection end time !v0n 11(41 12"b
-Grxab or Coomposite Z*'*
Temperature C°C) 0, V"C* . C . *'.
u on recei t AL - 0 TpAA(e.
Physical characteristics -""
CLQA.* Ci.eAe- 04 4*
. oi-ac- os-at--aC Dates sample used ETS Project and
Environmental Testing Solutions, LLC e'age / 01 ,5 Environmental Testin2 Solutions, LLC rage i 01 Species: Ceriodaphniadubia Client: "F
- P1 - SRAti\AAR Date: 6d-01-O-L-Daily Chemistry:
Concentration Parameter 0 1 CONTROL pH (S.U.) B Z1 z. . B. UP Conductivity --3 11 Temperature
__ _ 0_cC) vJ4.9 PH (S.U.) ,3 .z" 5Zq I DO (mgiL) .q
% Conductivity (gmhos/cm). _3 1 * --.-
- 2 q __ _ _ q Temperature :q CC) .16Zq 17 pH (S.U.) A.7(V ý,z DO (mg/L) 1,. 13-.
21 Q0 Conductivity (Wuhos/cm) 76 Temperature . ,.
(0c)
Dail Chem, pH:'.6 (S.U.) Z. a.q3 6 *.
DO (mgi'L) r4Z.8~
43 F)0 '% Conductivity (gumhoskm),. zS4 25o 3
- Z5.*
Temperature (0c zq.F: z 4*S 7 -m z*. M q.7l -24 pH (S.U.) 0.20 ". *. .l S 3.w 6.Z5 77 0 DO (maI) 8.Z :.1 .O600.
Conductivity 21Z (gmhos/cm) i 2- - z -3 Temperature G 0c), z4..
pH (S.U.)
DO (mg/L) 6-6 8-h (D09o Conductivity 14 7 (panhos/cni)
Temperature PH (S.U.) L03 fATK8 DO (mg/L)
Conductivity Temertus/cm)
Temperature 20 ZV, "
1-z Z'.'
31 CC) ii IInitial I Final Initial Initial 11Final B Final IInitial I Initial If Final Final I Initial B Final II
AL,"VjjUjjjjjUj&taj Species: Ceriodaphniadubia Client: TNIN Se9VAIAA- Date: 05. 01-0-L.
I iI-iiii+nn IPariampter 4fl** -.
1 4
3 4 D 5 6 CONTROL PH (S.U.)
DO (mg/L)
Conductivity 31q M1(zt (1unhos/cm)
Temperature
- 4i24.6 -Z.4.4 24I.9 z24, 5 t1 2.-Z.L Dom (In -o 2e Conductivity N iD.Cqell Temperature 2c..5 zs(ZL.)i~.
PH (S.U.) A- -V z-. eI-f5-3 Z 8.1 - W3 ZZ/a DO~mgL- P-0, ZZ
'~Conductivity Temperature -Z.P 2q~q z1 2S~.1 -Zq . zq.3 Z4LI.A
-oC -f 6 8z G 92 3 C0nuctvi0 PH (CU. e-2 Z4.1 6-- 8z Do (mg/o 5.1 -4. i.z 63.
Z1 ")
- 3. Conductivity 2~ ~
Teprtr VIi *(,qt2 25.1 95.0 V'1..(,0,14, z4,'
'2o Conductivity I1' Temperature zqqq z.I~iG t.( q PH (S.U. &0 IpjN~DO (mgL At2o. &.0L Conductivity -+5 Temperature 2LI ZI~2/& i- I Z.o~ C. q-'Id Condutivit I Iii3z.IS5 Initial r Inal 1W...] Titi-I Final
Environmental Testing Solutions, LLC 02, Methods 1000.0)
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/0 Species: Pimephalespromelas Summary of Chemical Analyses Client: Sequoyah Nuclear Plant UV-Treated Teat date: May 07-14, 2002 Reviewed by:
Project number: 324 Pimephalespromelas 24.31 24.2 25.01 24.8 24.61 24.5 J.Jl 0.2 24.2 25.3 24.5 0.2 Overall Temperature (C) temperatures)
(including all concentrations for initial and final
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant UV-Treated Test dates: May 07-14, 2002 Reviewed by:
Project number: 324 Day 4 D Day 6 Day 2 Day 33,2 Day. 8.Day Day 0 Day 1 7.72 Concentration Parameter Fia Initial Final initial Final ia nta Innia ia nFinal ina Fina Itil Inta 8.18 23 7.4 8.24 8.00 8.25 7.90 8.22 7.85 8.27 8.12 8.22 7.
pH (SU) 7.5 7.9 6.8 8.2 7.8 8.1 7.1 7.9 6.9 7.9 DO (mgfL) 7.9 7.6 8.1 310 03 296 Conductivity (pmuhos/cm) 315 63 64 65 Control Aalinity (mg/L CaCO3 )
89 82 Hardness (mg/L CaCO3) 8 24.6 24.8 24.3 24.5 24.3 24.5 24.6 24.5 24.4 24.6 24.7 24.3 Temperature (C) 24.5 24.3 7.6 8.211 7.481 8.2 8.241 7.94 8.21 7.8 8.17 8.2+8 8.12 8:.ý22 7.0 8.23 pH (SUJ) 7.9 6.87.747961 7.0 8.2 7.8 8.21 7.0 289 7.9 7.71 8. 299 289 287 288 10.98% DO (mgfL) (Rmhos/cm)
Conductivity 300 24.8 24.8 24.4 24.5 24.3 24.5 246 24.5 24.5 24.7 24.2 Temperature CC) 24.6 24.3 8.20 7.45 8.1 8.16 7.92 8.25 8.10 8.21 7.76 8.20 pH (SU) 6.8 777.4 7.8 6.5 8.0 . 8.11 7.31 7.91 7.9 7.7 8.0 7.0 270 274 274 DO (mg/L) 283 279 280 277 22% Conductivity (pmhos/cm) 24.2 25.0 24.8 24.4 24.5 2.5 24.3 24.5 24.5 24.7 4.6 24.7 Temperature (*C) 24.7 8.1 743 8.20 7.93 8.15 7.72812 7.96 8.24 8.10 8.20 7.70 8.161 7.94 pH (SU)" 7.6 8.2 7.4 7.9 6.9 7.4 7.9 7.7 .0 6.9 8.1 DO (mgfL) 251 48 253 249 240 43.9% Conductvty (pmhos/cm) 24.2 25.1 4.5 24.3 24.5 24.3 24.6 24.6 24.6 Temperature CC) 7.80 8.12 7.84 - 8.081 7.961 8.141 7.421 8.20 8.08 8.18 7.791 8.121 7.941 8.17
- pH (SU.) 7.3 8.1 7.5 8.1 7.0 7 DO (m*gL) 8.0 7.7 20 211 214 210 72% Conductity (pimhos/cm) 24.7 24.6 246 24.2 2 24.7 24.3 24.5 24.4 Temperature (CC) 8.08 8.15 7.71 8.06 7 pH (SU) 8.17 8.0 DO (mg/L) 7.9 164 Conductivity (jmhos/cm) 169 595 Alkalinity (mg/L CaCO3 ) 57 100% 76 Hardness (mg/L CaCO3) 7
<0. <0 Total Residual Chlorine (mg/L) 24.8 24.4 24.5 24.2 24.5 24.8 246 24.6 24 25.3 24.81 24.3 24.5 24.3 Temperature CC) 8.14 7.87 8.08 7.76 8.02 7.9 8.12 8.10 8.13 7.74 8.07 7.94 pH (SU) 8.14 6.8 8.0 7.4 7.9 8.0 7.6 8.1 7.1 8.0
- 7. 7.7 8.0 7. 167 DO (mg/L) 168 165 168 167 169 172 Conductivity (pinhos/cm) 19 100% Intake Alkalinity (mgfL CaCO3 )
72 Hardness (mg/L CaCO3) <0.10 <0.10 Total Residual Chlorine (mg/L) 24.5 24.2 25.0 24.8 24.4 24.5 24.3 24.3 24.7 24.6 24.7 "Temperature 24.5
Species: Pimephalespromelas Client: -- 'A- &`7_ -I A A Date: 0S6-O'-O2.
MAL Full-strengthChemistry: 6 10 0LL.
Parameter Sample Number: Control Batch:
1 2 3 oC-06-07 oq-o2. o"-iA.ca pH (S.U.) _" ______ &.. 2.- *.2' DO 7.0 (mg/L) "_,_ d.0 1.01 Conductivity 16qI 315 7-4, (o (pmhos/cm)
Alkalinity (mg CaCO3/L) 51 59 o* 64 aS Hardness (ma CaCOJL) 716 12_
Chlorine 40,10 -<0,10 0. 1o Collection start date O*O-Og-2 O*-O7&.oZ oS-*-oZ Collection end time J0 M MR Grab or Composite *o~i- 73 J&ot" (duration) LM49Oi Te CVt1rT- CoieM' Temperature (°C) 0 V upon receipt 0__,____.
Physical P.ALe -RM9 Ouet characteristics c -TA-4 C-LeM. Cj-j'h Dates sample used cco-OZ 0-Oq--z, as-&6
- oto -o1 z. .o-o2. %94Z~t-o 05ýt-7ýCK1205"?-0.
os-3-OZ.
ETS Project and 505(
Sample numbers ozowO7. 01 o205Oq.0 0 oZOI-.
OW! 9 Species: Pimephalespromelas Date: 0-'0 2.
Client:
Full-strength Chemistry: INhAKe Control Batch:
ieNmbr "
Parameter 1 2 3 pH. 9.l.0"1 C', o ~-O~ -119 argO
-7.9O DO -9q .0
- 315 ?9(0 p503 Conductivity 1" 16e umos/cm)
Alkalinity O t.::r Jq'j S9 Hardness -7 I'/ -1(
in aC__- -- ---
Chlorine 4 0110 xOJO 10 I40 Collection start date OZ--c*--oioCZ Collection -end time 11[c41I 12s Grab or Composit---e* . o_*3**
°Cce~e 4f (duraon Temperature CC) 0. 34c, 0. a-3C IX C upon receiRLtt M olt Physical fA/e "/W /qe 1' t characteristics C-.M. C.&- -rA.
2 -C" Dates sample used o0-'-
054O-02. ¢- 0qoý o-L 0"o osI9-12~q-a" 32-*oq 6251 0ows1 (.,a ETS Project and Sampe numbers zoTo
Li Species: Pimephalespromelas Client: TW/ Date: o*-01-0Z a .-TfA~~
oomtmus, t esun*
Environmental
- B T'! 1-f C' Page 8 ot 8 I*nviroflmeltaI iesting 301ULioiIs, Species: Pimephalespromelas Client: "r4A- SeLWAA Date: 650-7"-02---
_ 4aI Da Concentration CONTROL Parameter pH (S.U.)"* 3 "*'.*O I5e .. S *.00 *,Z-3".j6 DO mg)A Conductivity CL,030
_._o3_
( os/cmn ' .. -... -I"
- q. s Z4.5 2 q. "
wA~ Conductivity Temperature . q. q _...:
"DO (mgIL) 2-. o.
Conductivity PH~~ui (SU) 2861 Temperature z41 1 22q.zq3 zq. 2q zq.4. Z' S "Conductivity 9;70L Temperature 12 -Zq ' 2zq, 2 q.3 2q.5
( onduct) t . 241-H (S.U.) B i1+ -4..
/*0::o Conductivity
(,,mhos/cm) . eetle _(o5 I
Temperature zq.g. 7- 2(4/3 PH (S.U.) il et. 0--
DO (mg/L) j(00 rn'
- Jl~a Z Conductivity Ie1 Temperature 2 5.4 '?:. 2z..3 2 "
¢C*.... . '_^ ,:,:l Vn*! In tiahuial [Initial Final Initial f!inid I l'iI ."th t * . ----
Environmental Testing Solutions, LLC Page Page I of__J__
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Iodide Acid reagent:
reagent: I0IQp0q4 AlOV Analyst [Z I-I Slope: L Date analyzed 6fj-1 Calibration:
Refernce tun ar num er L e levels of th amls to bracket the chlo0rinelvsofteame.
1.0 mg1L. the calibration range must be adjusted Note: For samples with a residual chlorine of >
characteristics Residual chlorine I%RPD = {(S - D) IL(S+JJ)LLII X J`l (M (acceptable range -+/-~ 101%)
D4<0.00L40' 0 holding time (15 minutes) unless otherwise noted.
Note: All samples were analyzed in excess of EPA recommended Reviewed by I Date reviewed
Environmental Testing Solutions, LLC Page _____
Page ( ofJ1 Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pHI/lon Meter Iodide reagent:
Acid reagent:
Analyst 0-4 Slope:
Date analyzed _CK-1 -OZ Calibration:
. .... 0.10u gl 1.00 m/
Reference standard number j .A. _3J .I the chlorine levels of the samples.
mg/L, the calibration range must be adjusted to bracket Note: For samples with a residual chlorine of > 1.0 I
I Reviewed by [--1--2 Date reviewed
' O U
Environmental Testing Solutions, LLC Page ___
Page I_. of I Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Iodide reagent:
Acid reagent:
Analyst I II-II1 Slope:
Date analyzed C6-I1 0_-
D 0. ýo-2 I 6%) 1 noted.
holding time (15 minutes) unless otherwise
- were analyzed in excess of EPA recommended Reviewed by reviewed Date
Page jLOf .
Environmental Testing Solutions, LLC Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3 /L 7i et 4 Analyst [ji Titrate samples to pH 4.50 S.U.
Date analyzed [~~oJ =
Tit rann and multipfer determination:
PH of Normeality 'B~in DeIonized Titrant check Be reference standard MI n water 4.5 S.U. number number (arP Date reviewed: 6-Oo-Reviewed by: =
Environmental Testing Solutions, LLC Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [I& II Date analyzed 0[.gZ.o 2 Titrate samples to pH = 4.50 S.U.
0and multipier determination:
pH of II Normality (N) of HzSO 4 pH Factor or Multiplier Deionized Titrant check
- dIlgin--
Total = (5 ml Na 2CO 3 x 0.05)/E = (N x 50000)1 100 ml sample water reference standard ml ml =--0.25/E N x 500
= 4.5 S.U. number number __l (accep) e0.018 - 0.022)
Laboratorycontrol standard:
Reference True value Sample Alkalinity (MV) % RS = MV / TV x 100 I standard number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO03 L) (ml) ml ml ml 90 to 110%)
1,46sstq 1o 0 wo ooloI I00 ii 1, e 9 e Duplicatesample precision:
Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO 3/L) I(S - D) JI(S+D)12I) x 100 number (ml) ml ml ml L (acceptable range -:6 10%)
to o I q t " *56 "(00-4 ,. s 0, SoI.LO-*z ftDuplicate-14 C - D OS-14-07-O 1__ _ OO . Z55. 1.o ýq(* T D to"/
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (ing CaCO3/L)
"(mgCaCO3/L) (ml) ml ml ml 5U iou Z5.6 Ii 1.q .6 1lt Sample alkalinity (B) Measured spike value (MV) % R = MV I SV x 100 (ag CaCO3/L) MV = A - B (acceptable range (mg CaCO-VL) 75 to 125%)
(a*qb LA Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaC0/L) o S -0.2 SsvW z ..- kj*%.9 4.o. 3.z _3_Z_ _1
- - 06-o.o - 3-.
.6 __ _
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a Page -fiof Environmental Testing Solutions, LLC Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst [ C~
Date analyzed 06-L1).OZ Tirant no and mut-ipl-er d mination: Tma Begin End Total Normality (N) of EDTA Titrant Normality check ml ml ml O.21E
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Page 2- of LLC ZEnvironmental Testing Solutions, Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst [ o.*7--Z Date analyzed .i and muplti er determination:
in End Titrant Normality c e standard ml reference number number q-4
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Note: If > 15ml of titrant is used, sample must be diluted. Reviewed by: C/I \I
Sequoyah Nuclear Plant Biomonitoring May 7-14, 2002 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, LLC Toxicant Control Chart Potassium Chloride Chronic Reference for Pimephalespromelas using Moderately Hard Synthetic Water S CV Test date 7-day IC25 CT SA.75 SA.90 Test number (g/L KCI)
(g KCI/L) 1 06-13-00 0.70 0.26 0.31 0.05 0.68 0.03 09-19-00 0.66 0.32 '0.08 2 0.71 0.06 0.27 10-24-00 0.77 0.31 0.08 3 0.69 0.05 0.26 0.65 0.31 0.07 4 11-07-00 0.05 0.26 0.69 0.69 5 03-13-01 0.25 0.30 0.12 0.67 0.08 06-26-01 0.53 0.30 0.11 6 0.68 0.08 0.26 07-17-01 0.74 0.30 0.11 7 0.67 0.07 0.25 0.62 0.30 0.11 8 08-21-01 0.66 0.07 0.25 0.60 0.30 0.11 9 09-25-01 0.67 0.07 0.25 11-01-01 0.71 0.30 0.10 10 0.67 0.07 0.25 11-06-01 0.66 0.30 0.10 11 0.66 0.07 0.25 11-27-01 0.57 0.30 0.10 12 0.66 0.07 0.25 12-12-01 0.63 0.29 0.12 13 0.64 0.08 0.24 0.49 0.29 0.12 14 01-04-02 0.08 0.24 0.61 0.64 15 02-05-02 0.07 0.24 0.29 0.11 0.65 0.64 16 02-13-02 0.07 0.24 0.29 0.12 0.56 0.64 17 02-26-02 0.07 0.24 0.29 0.11 0.70 0.64 18 03-28-02 0.07 0.24 .0.29 0.11 0.61 0.64 19 04-23-02 0.07 0.24 0.29 0.11 0.54 0.64 20 05-07-02 An estimation of the concentration of Note: 7-d IC25 = 7-day 25% inhibition concentration.
reduction in Pimephales potassium chloride that would cause a 25%
growth for the test population.
CT = Central tendency (mean IC25).
S Standard deviation of the IC25 values.
S = Standard deviation corresponding to the the 7 5 th percentile CV.
8A.75 = 0.38, as determined by USEPA for the method and endpoint.
to the the 9 0 th percentile CV.
SA.9O = Standard deviation corresponding *÷ SA.90 = 0.45, as determined by the USEPA for the method ana enupu--L.
values.
CV Coefficient of variation of the IC25
=
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the 3 Agency, Cincinnati, OH.
EPA-833-R-00-00 . US Environmental Protection National Pollutant Discharge Elimination Program.
05-07-02.xls Inc.
Organisms obtainedfrom Aquatic BioSystems,
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 I I I I I I I I I I I I I I I I I ControlLimits Set According to +/- 2 StandardDeviations.
1.0 9=ý U
0.8 S .°.. . .
... ............... .- . . . . . . . . ....... °.................. .
.... I.........................
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'I Oi? 0 4 ? . . . ..
-'" Iv 1 1 1 '
% N1 %
Test date 0 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
- - Central Tendency (mean IC25)
......... Control Limits (mean IC25 +/-2 Standard Deviations)
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 2 .0 I I i I I I Warning and Control Limits Set According to 75th and 90'h Percentile CVs.
1.5 1.0 ........ ........ . ....
S0.5 0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.11) is less than the 7 5th and 90d percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC25)
-... -Warning Limits (mean IC25 +/- SA.75, SA.75 = 0.38, as determined by USEPA for the method and endpoint)
..... Control Limits (mean IC25 +/- SA.90, SA.90 = 0.45, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Control Control Mean MSD PMSD CT Survival Growth CV CT Test number Test date for Control for PMSD (%)
(%)
(O/) (mg/larvae) (%) Growth CV (o) 0.58 13.2 0.12 20.0 1 06-13-00 100 0.07 11.2 0.67 5.3 15.6 09-19-00 100 9.2 26.9 19.4 2 13.3 10.6 0.22 97.5 0.83 19.3 19.3 3 10-24-00 10.5 10.6 0.13 97.5 0.67 20.0 4 11-07-00 9.8 0.08 22.8 0.34 6.5 5 03-13-01 92.5 9.8 0.19 30.6 21.8 0.63 9.8 06-26-01 100 0.07 13.8 20.7 6 9.1 9.7 100 0.52 20.0 7 07-17-01 9.5 0.13 15.1 0.89 8.3 8 08-21-01 100 9.0 0.09 10.4 18.9 0.85 4.9 9 09-25-01 100 8.3 0.10 18.6 18.9 0.54 2.5 11-01-01 100 0.17 16.9 18.7 10 7.4 8.3 100 1.00 11 11-06-01 9.5 0.18 26.1 19.3 0.70 22.8 12 11-27-01 97.5 10.0 0.19 23.5 19.6 0.82 16.6 12-12-01 95 0.18 22.0 19.8 13 10.1 10.0 95 0.80 20.0 14 01-04-02 6.9 9.8 0.21 22.2 97.5 0.95 19.7 15 02-05-02 8.0 9.7 0.10 15.5 100 0.65 19.5 16 02-13-02 3.3 9.3 0.11 16.5 100 0.64 19.6 17 02-26-02 11.2 9.4 0.13 21.4 100 0.63 18 03-28-02 9.6 0.19 26.2 20.0 0.71 12.8 19 04-23-02 100 0.07 15.4 19.7 12.3 9.7 97.5 0.46 20 05-07-02 Note: CV = Coefficient of variation for control survival.
Testing Solutions, LLC Pimephales chronic On average, the CV for control growth is 9.7% in Environmental toxicity tests.
3.5%.
Lower CV bound determined by USEPA (10" percentile) =
20%
Upper CV bound determined by USEPA (901P percentile)
MSD Minimum Significant Difference PMSD = Percent Minimum Significant Difference minimum percent difference between the control and PMSD is a measure of test precision. The PMSD is the significant in a whole effluent toxicity test. On average, a significant treatment that can be declared statistically LLC chronic toxicity tests when a toxicant reduces difference occurs for Environmental Testing Solutions, Pimephalesgrowth by 19.7% from the control.
= 9.4%.
Lower PMSD bound determined by USEPA (10"h percentile)
(90"h percentile) 35%.
Upper PMSD bound determined by USEPA mean PMSD)
CT = Central Tendancy (mean Control Growth CV or growth in chronic reference using 205 tests conducted from 19 laboratories for Pimephales The lower and upper bounds were calculated by the USEPA toxicant tests.
Under the National Pollutant Variability in Whole Effluent Toxicity Applications USEPA. 2000. Understanding and Accounting for Method Environmental Protection Agency, Cincinnati, OH.
Discharge Elimination Program. EPA-833-R-00-003. US 05-07-02.xls
- . . - I . - I 1.- 4-- r"I
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 40 30
~20 10 0
Test date
- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
S- -Central Tendency (mean PMSD)
. ........Lower and Upper PMSD Bounds Lower PMSD Bound ( 101h percentile) = 9.4%, Upper PMSD Bound (90 te percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Page 01or5 Ys~.
T T ironmental leslig Solutionsu, Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number: 18 w w'eAIVN rIomns Comments:
Dilution preparationinformation:
KCI CHM number: rul H 0J1 Stock preparation: 50 g KCI/L: Dissolve 50 g KCI in I-L Deionized water 300 450 600 750 900 Dilution prep (mg/L) 6 9 12 15 18 Stock volume (mL) 994 991 988 985 982 Diluent volume (mL)
Daily feeding and renewal information:
Morning feeding Afternoon feeding Test initiation, renewal, Analyst Analyst Day Date Morning feeding Afternoon feeding or termination time time time 0 0g-C7-07. l(000 1
-CC08-O'L i 1014 16o 2
C-S-10oL sIzo 3 0145s. 1606
)oooJ51 001b Ito /c 4
5 05.10-0-OL AL/ K, 6 j%, tS -6"2 7 1
Page 3 of 5 vironmental Testing Solutions, LLC PpK C1CR T est N umber: I _ _
I4owwwwry b Calculations and data reviewed:
I
Environmental Testing Solutions, LLC Method 1000.0)
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses PpKCICR # 18 May 7.14, 2002 0.0707 MSD = Minimum Significant Difference Dunnett's MSD value: PMSD = Percent Minimum Significant Difference 15.4 percent difference between the control and treatment that can be PMSD: PMSD is a measure of test precision- The PMSD is the minimum toxicity test. On average, a significant difference occurs for Environmental declared statistically significant in a whole effluent Pimephales growth by 19.7% from the control (determined Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Lower PMSD bound determined by USEPA (10th percentile) = 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) = 35%. in 205 tests conducted from 19 laboratories for Pimephales growth The lower and upper bounds were calculated by the USEPA using chronic reference toxicant tests.
US Environmental Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Protection Agency, Cincinnati, OH.
05-07-02.xis Organismsobtainedfrom Aquatic BioSystems, Inc.
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Sample 1D0 REF-Ref Toxicant Start Date 517/02 Test aD PpKCICR Sample Type KCL-Potamsiu= chloride End Datat 5/14/02 Lab 1D: ETS-Env. Testing Solutions Test Species: PP-Pimephales promelas Sample Dam Protocol: EPAF 91-EPA Freshwater Cooe-mg/L 1 2 3 4 D-Conml 1.0000 0.9000 1.0000 1.0000 300 1.0000 1.0000 0.9000 1.0000 450 1.0000 1.0000 1.0000 1.0000 600 0.80 0.7000 1.0000 0.8000 750 0.8M0 0.9000 0.8000 0.8000 900 02000 0.3000 0.4000 0.1000 1-Tailed Number Total Rank Transeform: Arcsin Square Root Resp Number CV% N Sum Critical N-Mean Mean Mm Max 40 Conc-mg/L Mean I 1.2490 1.4120 5.942 4 D-Control 0.9750 1.00 1.3713 1 40 5.942 4 18.00 10.00 1.0000 1.3713 1.2490 1.4120 0 40 300 0.9750 4 20.00 10.00 1.4120 1.4120 1.4120 0.000 40 450 1.0000 1.0256 10.00 7 1.4120 15.615 4 12.50 0.8250 0.8462 1.1544 0.9912 7 40 600 6.209 4 10.50 10.00 0.1462 1.1426 1.1071 1.2490 30 40 750 0.8250 4 10.00 10.00 0.2564 0.5124 0.3218 0.6847 30.428
- 900 0.2500 Critical Skew Kurt Statisc Amillacy Tet 0.114 0.427279336 1.241498999 0.948615253 Shapiro-Wllh sTeat indicates rotmal distribution (p>0.0I)
Equality otvnmmrcau-eot be confirmed NOEC LOEC ChV TU Hypothesis Test (1-tal, 0.05) 750 900 821.5838363 Stee's Many-Ooe Rank Test Maximum Llkelllood-Probit Sigma lter Chi-Sq Critical p-value Mu SE 95% Flduclal Limits Control 22 Parameter Value 7.814724922 0.47 2.930516378 0.040272093 0.025 2.535851211 Slope 24.83109115 6.391501454 12.30374806 37.35843424 Intercept -67.76791931 18.77373555 -104.5644417 -30.97139691 /
1.0 TSCR. 0.099690478 0.023695382 0.053247527 0.14613342a sg/L 95% Fiducial LimIls 0.9 Peint Probitsl ECO1 2.674 686.7987265 537.058375 747.6565068 0.8 "EC05 3.355 731.6016577 600.8859727 781.3018378 0.7 ECI0 3.718 756.6694322 650.5914712 800.3850597 ECI5 3.964 774.064301 680.0050895 813.9142816 0
EC20 4.158 781.1747746 704.0221999 825.1876413 0.5 EC25 4.326 800.4151229 724.9761854 835.3575863 4.747 832.3645221 778.0726786 864.3273455 5 04 EC40 ECS0 5.000 852.150649 808.6100921 885.7921691 912.3091936 0.3 EC60 5.253 872.4071154 836.1133631 0.2 EC75 5.674 907.1508087 873.6396115 969.6457235 5.842 921.319425 886.269403 996.4079068 0.1 ECSO EC85 6.036 938.1142293 900.0531996 1029.871372 0.0 EC90 6.282 959.6815291 916.5147209 1074.967135 100 1000 10000 10 EC95 6.645 992.5629769 940.0032492 1147.249305 983.3092802 1299.356351 Dose mg/
EC99 7.326 1057.312313 Dose-Response Plot 0.0
-0.8 0.
0.5
,-0.3 0.2 0A oI*
n'Z n7".Al" ýlc
Environmental Testing Solutions, LLC Statistical Analyses Larvall Fish Growth and Survival Test-7 Day Grow Sample ID: REF-Ref Toxicat 537/02 Test ID: PpKCICR Sltat Dot= KCL.PotasiWm chloride ETS-Env. Testing Solutions Sample Type:
End D=s 5114/02 Laba.
Test Species: PP-pimephales prmelas Protocol: EPAF 91-EPA Freshwater Sample Da=e Coumenas:
Co-esmg/L 1 2 3 4 0.4440 0.3872 0.5186 0.4822 DContol 300 0.4208 0.4538 0.4311 0.4935 450 0-3496 0.4177 0.3787 0.4287 600 03425 0.2740 0.2925 0.3473 750 0.2579 0.3274 0.2673 0.3636 0.0630 0.0822 0.1126 0.0390 900 I-Tiled Isotonic Transform: Untransformed Mean N-Mean N t-Stat CriUeal WSD Mean Niln Max CV% 1.0L00 con il Mean N-Mesn 0.4500 0.3872 0.5186 12.265 4 D.Control 0.4580 1.0000 0.4580 2.410 0.0707 0.4490 0.9821 0.4935 7.165 4 0.279 0.4498 0.9821 0.4498 0.4200 0.0707 0.3937 0.8596 300 9.241 4 2.192 2.410 0.0596 0.3937 0.3496 0.4287 03141 06.38 450 0.3937 4 4.904 2.410 0.0707 0.3141 0.2740 0.3473 11.602 0.6639 "600 0.3141 0.6850 5.245 2.410 0.0707 0.3041 0.2579 0.3636 16.523 4
- 750 0.3041 0.6639 0.3041 2.410 0.0707 0.0742 0.1620 0.1126 41.924 4 13.076 0.0742 0.1620 0.0742 0.0390 "900 Critcal Skew Kurt Stastsc Asuy T 0.084 0.025996871 -1.030953136 tisibodon (p> 0.01) 0.956860721 ShApim.-ilas Test iseg -W 15.08631706 1.646460414 Bha oilee s indicates ep-A Test e vatiances (p - 0.90) MSB WE F-Prob O ChV TV WDU MSDp NOEC LOEC Hypothesis Test (1-t4l, &.05) 0.154442041 0.000935563 0.001022691 1.1E-09 3,10 450 600 519.6152423 0.070734453 Dunnetfs Tea Linear Interpolation (80Remmples) mg/L SD 95% CL(KEp) Skew Point 339.29 88.10 0.00 449.63 -0.8574 IC05 400.49 63.04 140.29 517.97 .0.7585 ICIO 277.09 543.09 -0.9195 1.0 IC15 458.24 47.77 38.76 363.05 585.41 -0.5792 0.9 IC20 501.40 41.44 438.65 635.04 1.2997 0 IC25 544.55 769.09 26.54 635.98 795.77 -2.208l 0.0 IC40 7990 1 1.39 7 33.26 024.51 .0.2200 0.7 w 0.6 0.5 0.4 0.3 0.2 0.1 0.0[2 200 400 600 800 1000 0
Dose mg/L Dose-Rtesponse Plot 0.6 0.5 0.05 level 1-tail.
04--------------------------------------------------------------
.. .of . . significance a 0.3
- 0.2 0.1 0
o S A- A A- ..I
,ironmental Testing Solutions, LLC PpKCICR Test Number: I&
f,)ONMURA r'etb MHS Control Chemistry:
Parameter Control Batch:
osN-e 05-0-0 o- s*
pH PHu , g2 - a. R.IZIS (S.U.)__ _ __ _ _ _ _
DO (mg/L) -_. _ _,__.
Conductivity (gmhos/cm) 3Z 3tlI Alkalinity (mg CaC0 3/L) 6`5 V4____
Hardness r(mg CaCO 3/L) _ _ _ _ ____- _
Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 300 mg KCI/L 840 - 890 450 mg KCI/L 1100-1180 600 mg KCIJL 1390- 1460 750 mg KCI/L 1660-1710 900 mg KCIlL 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCI1/L)
Page D01 pvironmental Testing Solutions, LLC PpKCICR Test Number:
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ceatration Parameter 345Z NTROL pH (S.U.) 7I~ 8-26 f?
DO (mg/L~) 60S Conductivity 3~ a (limhos/cm)Iq Temperature A-
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uig KCIfL Conductivity (gmnhos/cm) I]qz IN 1 Temperature 246 4-Z z4.1T 2'14 zzPI 4 q.c PH (S.U.) 8-1 4 G N3 e, .4823 mg KCIIL DO (m&UL (0.
Conductivity Ounlios/cm) jLJOZ Temperature 1-9461 z'4.? zq4q L2-11 z 24.q OH (S.U.) A.7-(.0 9 -4.81 i
1 11 II IqB1 I I fa.za
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)00 ing KCI/L Conductivity (gmnhos/cm) / I Temperature Initial Ii Final I initial I Final I Initial II Final 1 Initial HFinal
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Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water CT S SA.90 Cv Test number Test date 7-d IC25 SA.75 (g/L NaCI) (g/L NaCI) 1 12-17-00 0.89 0.59 0.09 0.43 2 12-19-00 1.01 0.95 0.08 0.06 0.06 0.43 0.59 12-19-00 0.97 0.96 3 0.43 0.59 0.05 0.95 0.95 0.05 4 01-09-01 0.44 0.61 0.08 1.11 0.99 0.08 5 02-06-01 0.62 0.08 0.99 0.08 0.45 6 03-07-01 1.04 0.07 0.07 0.45 0.62 04-04-01 1.02 1.00 7 0.46 0.63 0.07 1.10 1.01 0.07 8 05-09-01 0.63 0.07 1.02 0.07 0.46 9 06-06-01 1.07 0.07 0.07 0.46 0.63 07-10-01 1.05 1.02 10 0.46 0.63 0.06 1.02 1.02 0.07 11 08-08-01 0.63 0.06 1.02 0.06 0.46 12 09-12-01 1.02 0.06 0.06 0.46 0.63 10-10-01 1.03 1.02 13 0.46 0.63 0.06 1.00 1.02 0.06 14 11-01-01 0.63 0.06 1.02 0.06 0.46 15 12-05-01 1.05 0.06 0.06 0.46 0.63 01-09-02 0.91 1.02 16 0.46 0.63 0.06 1.07 1.02 0.06 17 02-05-02 0.63 0.06 1.02 0.06 0.46 18 03-19-02 1.03 0.06 0.06 0.46 0.63 04-09-02 1.03 1.02 19 0.46 0.63 0.06 1.05 1.02 0.06 20 05-07-02 of Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
SA.75 = Standard deviation corresponding to the the 75th percentile CV.
SA.75 = 0.45, as determined by USEPA for the method and endpoint.
SA.90= Standard deviation corresponding to the the 9 0 1h percentile CV.
SA.90 = 0.62, as determined by the USEPA for the method and endpoint.
CV Coefficient of variation of the IC25 values.
in Whole Effluent Toxicity Applications Under the USEPA. 2000. Understanding and Accounting for Method Variability US Environmental Protection Agency, Cincinnati, OH.
National Pollutant Discharge Elimination Program. EPA-833-R-00-003.
05-07-02.xls
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.50 1.25 1.00
- 0 0.75 0.50 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC25)
Control Limits (mean IC25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 2.5 I I I I I I I I I I I I 1 I I I 2.0 Warning and ControlLimits Set According to 75th and 90eh Percentile CVs.
Q 1.5 z
1.0 t
IA 0.5 0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.06) is less than the 75"' and 90"' percentiles determined by the USEPA. these percentiles were used in setting the warning and control limits.
- I I, , , I I I I I I I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 '-+/-SA.75, SA.7S = 0.45, as determined by USEPA for the method and endpoint)
Control Limits (mean IC25 +/- S A.90 SA.90 = 0.62, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Watei Test number TControl Survival Control Mean Reproduction CV CT MSD PMSD for Control
(%) Reproduction (%) for PMSD (%)
(%) (offspring/female) CV (%)
12-17-00 100 25.6 4.6 3.3 12.9 18.4 1 9.5 4.6 15.6 12-19-00 100 24.8 7.1 2 11.1 5.0 19.8 17.0 100 25.1 19.2 3 12-19-00 10.4 3.5 12.4 15.9 100 28.0 8.4 4 01-09-01 3.1 14.2 15.5 22.1 10.1 10.4 5 02-06-01 100 10.1 14.6 8.3 10.0 2.4 03-07-01 100 24.0 6 10.0 2.9 11.8 14.2 100 25.0 10.0 7 04-04-01 3.1 11.0 13.8 27.9 8.9 9.9 8 05-09-01 100 12.5 13.7 5.8 9.4 3.5 06-06-01 100 27.8 9 9.4 2.8 11.2 13.4 100 25.0 9.4 10 07-10-01 2.7 8.7 13.0 30.9 2.4 8.8 11 08-08-01 100 9.4 12.7 6.6 8.6 2.7 09-12-01 100 28.6 12 2.5 9.9 12.5 24.9 8.8 8.6 13 10-10-01 100 11.6 12.4 6.5 8.5 3.1 11-01-01 100 27.0 14 8.5 4.8 19.5 12.9 100 24.8 9.1 15 12-05-01 8.4 4.5 16.3 13.1 100 27.5 7.5 16 01-09-02 8.5 2.6 11.1 13.0 100 23.1 10.1 17 02-05-02 3.0 11.2 12.9 26.3 8.6 8.5 18 03-19-02 100 10.7 12.8 12.3 8.7 2.8 04-09-02 100 26.2 19 8.7 2.3 8.4 12.6 100 27.3 8.1 20 05-07-02 Note: CV = Coefficient of variation for control reproduction.
Solutions, LLC On average, the CV for control reproduction is 8.7% in Environmental Testing Lower CV bound determined by USEPA (10'h percentile) = 8.9%,.
Upper CV bound determined by USEPA (90th percentile) = 42%
UMSD Significant Diference
= Minimum PMSD = Percent Minimum Significant Difference difference between the control PMSD is a measure of test precision. The PMSD is the minimum percent toxicity test. On average, a and treatment that can be declared statistically significant in a whole effluent Testing Solutions, LLC chronic toxicity tests when a significant difference occurs for Environmental toxicant reduces Ceriodaphnia reproduction by 12.6% from the control.
Lower PMSD bound determined by USEPA (10h percentile) = 11%.
Upper PMSD bound determined by USEPA (90"' percentile) 37%.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD) conducted from 33 laboratories for The lower and upper bounds were calculated by the USEPA using 393 tests Ceriodaphniareproduction in chronic reference toxicant tests.
in Whole Effluent Toxicity Applications Under the National USEPA. 2000. Understanding and Accounting for Method Variability Environmental Protection Agency, Cincinnati, OH.
Pollutant Discharge Elimination Program. EPA-833-R-00-003. US 05-07-02.xis
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 45 40 35 30 25 20 15 10 5
0 Test date
-- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
S- -Central Tendency (mean PMSD)
...... Lower and Upper PMSD Bounds Lower PMSD Bound (10"' percentile)= 11%, Upper PMSD Bound (90"' percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Page 1 otr7 J+/-iII mnnfal UAIhIIt,*1t441 VIIa~llL Tpgtinu
,.J'.*fl Snititions. LLC JJiLVl U lxxxx i tzAAtO. . ... 9v. .
Sodium Chloride. Chronic Reference 'oxicant T'est (EPAI600/4-91/002 Method 1002.0)
Species: Ceriodaphniadubia rest Number:
Date Time Aalsyst CdNaC -1ICR ._
Teststart OS-L"6?0" n 12 - 4X \
Test end 6s.t4 -
Comn Comments:umts:
Dilutionpreparationinformation:
NaC1 CHM number: CA*&
100 g NaC1/1 (dissolve 50 gNaCl in 500 ml Stock preparation: deionized wtr 600 800 1000 1200 1400 Dilution prep (mg/L)
Stock volume (mL) 9 12 15 18 21 A1 IA8.8 1485 1482 1479 LDlluent VOIUmeIkmlh) I '-'1 ,l.I Test organism information: Tt rmatin:
Organism age: 4z-c q .-
% Randomizing template: -'ýOe4.
times organisms cS'-OL.0(-.-z 10 \-rc tcO7-- Incubator number: 2 Date and were born between:
Organism source: O' 0- o0 P-- -- - YCT batch: .... q4
, 4,0O2 Transfer bowl information: [pH = C.2-5 Temperature (°C)=t4., Selenastrum batch: AS aO-1,4.2, CONTROL Survival and Reproduction Data D ay 1 23 4 Replicate. ..number 6 - ," - ] - -
-*9... 10 1 Young produced (N t 0 0) C 0 (
- V
- L.- . (4- .
Adult mortality \.. L z Yon prod
=puced )IOIo___I©_O_ 0*
Adult mortality --
S Young produced Adult mortality 0~j 0 "
0
- j7i+
~ L 4 Young produced I l o Adult mortality _I_' '" - . -- - - -.
5 Young produced8 Adult mortality I - L.
I- __ -- _ "
Young producedil 0 0y l Ic o 4 i1j Total young produced 2) *Q j Zt Z9C) 2o Final Adult Mortal1 - . -:- - -_
-htn reviewed:
X for3r, Broods - -<n Test Renewal, Feeding, and Radomization Record Label Mon Tue Wed Thu Fri Sat Sun 1 Date "A O5S-alff1 " 0 -5 ()" ()3-&
Time !r lza 1/51 im I i1 MV U51 I~
Analyst Shelofat __
ILocation Acceptance criteria Summaryo test enypoints (mg NaC1/L):
Control information: _20% 7-day LCS0 .__ 0_
_q _
% of Male Adults:
% Adults having 3' Broods:
II5II 160% >80% NOEC
- 20% LOEC /000
% Mortality: _ 15.0 offspring/female" ChV oqq.
Mean Offspring/Female:
___ < 40.0 % IC25 _ 0qq. 2-
%NCV: I I
page 2 of 7 Environmental Testing Solutions, LLC CdNaCICR Test Number:
Concentration: _. i
%Mortality: ._ 2 M~ean Off'print1/Fiemale:
% Rduc:ti om Control:
Calculations and data reviewed:
Concentration:
% Mortality:
Mean Offspringeemale: 2
% Reduction from Control: 7 I Calculations and data reviewed:
Page :3 ot 7 Qnlii*inne I .T.r J~ilIIUIIU* Ii £*IV +.
- Q U~l *;" v o 1.f
.. I CdNaCICR Test Number: "."L-1AflfA m,~ Naoi'lr. Survival and Reproduction Data S Day Raab at 1 2 3 1 4 Replicate number 5 6 7 8 9 10 1 Young produced r--- -* -
Adult mortality -- .- . -
Young produced 0 an r 2
Adult mortaity - tL -1L-- ~ __- '- t -1' 3 Young produced 0 1 4I0 zI 0 Adult-mortality _ I L -.. L - L t L-4o Young produced , ,A 21 D 5Young produced C)C tion : 0 AdultmortalityM y 6 Young produced mortality [Q4 7 L.--** 7 ** k. L[- ICo10
- k. S Adultmortality [_ '-_=
- L-L,.J _ tL 7 Youngproduced -I QO I IO 0 Total young produced 1 I7 2IZ 1204 -25 2T-o_17Z wI 03 Final AdultMortali* t]* i I - "- I * -1 -i '1 Concentration:
%Mortality: 0-,
Adutmrtaft .. U. L__U. Mean - -I Offspring/Female:
% Reduction from Control:
-'I '
I3. 6 I
I u'B7.
Calculations and data reviewed: 4 1200 mg NaCII Survival andReproduction Data
__________ _____Replicate number___
1 Dy2 Young produced Adult mortality
[91~~ _
3
~
14~
5 117~L.-
16 718 9 10 2 Young produced p01 OF-- 0 Io 6 r~C)
Adult mortality IL - L.-..*
I I7-L-- - -I - -- _ L__
3 Young produced 0 2- __110 C Ada mortality -cz-t I~1 ~I'- U LI_ .. U 4 Young produced (o0 0~ ~ ~ .LI~ C Adult mortality L- .- - l - . -[U.~ - I~
6 Young produced I__
Adult mortality %___ A.-__
7 Young produced 0 1, I UIL Tu oprt duae l y o 51 0 1 zo 1 1- q1 FInal Adult Mortality T-. T- L-W I~ k.- - K Concentration:
I%Mortality:G%
%Redutionfrom Control:
Calculations and data reviewed: _*
Page 4 of 7 JIRVIIRURJIiULaLLa l JL a- I I CdNaCICR Test Number: -Z..Z-S,jrvjvaI and Renroduction i)ata S number 3 4 5 -6 7- 8 9 10 Day1 2-Q j C) 00 I Young produced -)
R[-
Adult mortality I L-2 Young produced jC)7 ~ ~ o~J I Adultmortality \-4_ _ I__ _,-. L-- I1L L 3 Young produced c- C C) 4 Young produced 0 0 Adult Fnl Adult Mormortalityf__
fitalty. ' _ __. onol _
',_. t.. .__I,.
6 produced uYoung I Adult mortality Adul morrtalyity 7JYoung produced Q Younging/Fmalec
, iea Total young produced 1.0 33s .1 '-41 1Final Adult Mortality Concentration:
[%Mortality:
Mean Ofsring/Femfale: f
.1Reduction from Control: &0 -6%
Calculations an(] data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #22 Revelwed by: /--
Test dates: May 7-14, 2002 I _-/I Survival Average reproduction Coefficient of Percent reduction from Concentration Replicate number variatn control 6 7 8 9 10 (%) (offspring/female)
(mg/L NaCI) 1 2 3 4 5 31 24 28 25 25 100 27.3 8.1 Not applicable Control 28 27 30 27 28 28 26 26 27 100 26.1 6.4 4.4 600 27 24 27 28 23 25 25 28 26 22 100 25.2 8.1 7.7 800 27 27 25 26 24 22 23 23 21 23 100 23.6 7.0 13.6 1000 24 24 22 24 25 27 17 9 4 9 100 10.9 47.9 60.1 1200 16 11 8 5 10 20 8 5 7 4 90 5.3 43.6 80.6 1400 2 8 5 8 3 3 Dunnett's MSD value: 2.866 MSD = Minimum Significant Difference 10.5 PMSD = Percent Minimum Significant Difference PMSD: can be declared PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that a significant difference occurs for Environmental Testing Solutions, LLC chronic statistically significant in a whole effluent toxicity test. On average, toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 12.6%/ from the control.
Lower PMSD bound determined by USEPA ( 10 'b percentile) = 11%.
Upper PMSD bound determined by USEPA (90th percentile) = 37%.
reproduction in chronic The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reference toxicant tests.
Discharge Elimination Program. EPA-833-R-00-003. US Environmental USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Protection Agency, Cincinnati, OH.
Environmental Testing Solutions, LLC Statistical Analyses Cerlodaphunla Survival and Reproduction Test-Reproduction Sample i1D: REF-Ref Toxicant Start Daec 5n1702 Test ID: CdNaCICR ETS-Env. Testing Solutions Sample Type: NACL.Sodium chloride End Date: 5/14/02 Lab ID:
EPAF 91-EPA Freshwater Test Species: CD-Ceriodaphnia dubia Sample Date: Protocol:
Comments:
5 6 7 9 9 10
-Conc-mgIL 1 2 3 4 28.000 31.000 24.000 28.000 25.000 25.000 D-Control 28.000 27.000 30.000 27.000 23.000 25.000 28.000 26.000 26.000 27.000 600 27.000 24.000 27.000 28.000 24.000 22.000 25.000 20.000 26.000 22.000 800 27.000 27.000 25.000 26.000 25.000 27.000 23.000 23.000 21.000 23.000 1000 24.000 24.000 22.000 24.000 l0.000 20.000 17.000 9.000 4.000 9.000 1200 16.000 11.000 8.000 5.000 3.000 3.000 8.000 5.000 7.000 4.000 1400 2.000 8.000 5.000 8.000 Rank I-Tailed Ilsotontk Transform: Untransformed N Sum Critical Mean N-Mean N-Mean Mean Min Mal CV%
Conc,-mg/L Mean 27.300 1.0000 27.300 24.000 31.000 8.108 10 D-.Control 27.300 1.0000 26.100 0.9560 28.000 6.373 10 88.50 75.00 600 26.100 0.9560 26.100 23.000 80.00 75.00 25.200 0.9231 25.200 22.000 28.000 8.111 10 800 25.200 0.9231 23.600 0.9645 27.000 6.977 10 62.50 75.00
- 1000 23.600 0.8645 23.600 21.000 55.00 75.00 10.900 0.3993 10.900 4.000 20.000 47.857 10
-1200 10.900 0.3993 5.300 0.1941 l0 55.00 75.00 "1400 5.300 0.1941 5.300 2.000 8.000 43.619 Critical Skew Kurt Statistic Auxliary Teats 0.79925704 1.035 0.46940546 1.0849612 Kolmogorv D Test indicates normal distribution (p >0.0) 20.2808399 15.0863171 Bartletez Test indicates unequal variances (p = 1.11E-03)
NOEC LOEC ChV TU Hypothesis Teat (1-tlaO 0.05)
Steels Many-One Rank Test 800 1000 894.427191 Linear Interpolation (80 Resamples)
Point mg/L SD 95% CL Skew IC05 636.666667 155.017213 358.664431 085 .33333 -0.2340 ICIO 878.75 94.9980011 668.58167 1004.25561 -1.0865 831.597222 1025.6365 -3.3118 1.0, ICIS 1006.22047 47.7651403 IC20 1027.71654 11.7313368 1004.79496 1050.25581 -0.0532 0.9 IC25 1049.2126 12.28073 1025.37009 1076.86047 0.2916 17.0805645 1095.25067 1153.5577 0.8164 0.0 1C40 1113.70079 1156.69291 21.6175266 1131.84389 1207.59528 0.8727 0.7 IC50 I* 0.6 o0.5 0.4 0.3 0.2 0.1 0.0 500 1000 1500 0
Dose mg/L Dose-Response Plot 35 30.
25 20 115 10 5
0
Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation only. Cerlodaphnla Survival and Reproduction Test-Reproduction Sample ID: REF-Ref Toxicant Start Date: 5/7u02 Test ID: CdNaCICR ETS-Env. Testing Solutions Sample Type: NACL-Sodiumn chloride End Date: 5/14(02 Lab ID:
Test Species: CD..Cerodaphnia dubia Sample Date: Protocol: EPAF 9 1-EPA Freshwater Comments:
6 7 8 9 10 1 2 3 4 5 Conc-mg/L 28.000 31.000 24.000 28.000 25.000 25.000 D-Control 28.000 27.000 30.000 27.000 25.000 28.000 26.000 26.000 27.000 27.000 24.000 27.000 28.000 23.000 600 22.000 25.000 28.000 26.000 22.000 800 27.000 27.000 25.000 26.000 24.000 27.000 23.000 23.000 21.000 23.000 24.000 24.000 22.000 24.000 25.000 1000 9.000 10.000 20.000 17.000 9.000 4.000 1200 16.000 11.000 8.000 5.000 3.000 8.000 5.000 7,000 4.000 2.000 8.000 5.000 8.000 3.000 1400 I-Tailed Transform: Untransformed CV% N t-Stat Critical MSD Mean N-Mean Mean MWn Mal Coac-mg/L 1.0000 27.300 24.000 31.000 8.108 10 D-Control 27.300 6.373 10 0.958 2.287 2.866 26.100 0.9560 26.100 23000 28.000 600 2.866 28.000 8.111 10 1.676 2.287 800 25.200 0.9231 25.200 22.000 6.977 10 2.953 2.287 2.866 23.600 0.8645 23.600 21.000 27.000
- 1000 2.866 10 13.087 2.287 "1200 10.900 0.3993 10.900 4.000 20.000 8.000 47.857 43.619 10 17.556 2.287 2.866
- 1400 5.300 0.1941 5.300 2.000 Critical Skew Kurt Statistic Auxiliary Testa 0.79925704 1.035 0.469405458 1.884961203 Kolmogorov D Test indcate normal distributio n 1p,> 0.u 1, 15.08631706 (p 1.11E-03) 20.28083992 MSB MSE F-Prob df Bartetefa Test indicates unequal variances = ISDp NOEC LOEC ChV TU MSDu M4964213 857.9466667 7.851851852 5.6E-27 5,54 Hypothesis Teat (1-talt 0.05) 800 1000 894.427191 2.865523013 0.104 Dunnetts Test f'.
-* tt n,q IIII.?
znvir~lOntuentati-tl ebtilig 00OIU0lll09IX_
CdNaC1CR Test Number: _7.
MHS Control Chemistiry:
Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria remade if the (dilutions must be outside Concentration conductivity falls the CONTROL 280-360 600 mg NaCI/L 1390 - 1590 800 mg NaCIIL 1780 - 1970 1000 mg NaClfL 2190 -2370 1200 mg NaCI/L 2550 - 2740 1400 mg NaCIJL 2910 - 3160 NaCI Stock 110500 - 116300
CdNaC1CR Test Number: 2 S. I o I
5ýý /Il1cv 11 DSbo I Dlgqoo
Environmental Testing Solutions, LLC Page 7 ot 'I Environmental TestingSolutions,_LLC Page 7 ci -I CdNaC1CR Test Number. _7, Day Concentration Parameter 3 4 5 6 PH (S.U.) 1b. Z -Z -3 DO (mgL 2 .
CONTROL Conductivty3 Temperature 743 Z. 45 -q- 4 45 242.
PH (S.U.) IF.V Z(. Z 4.3 rsl-z- &Z- 8.?z. 847-z DO (g/U S.0 13.1 600 mg NaCIIL Conductivity (grahos/cr) 5 I'195 Temperature zi4 zq 800 mg NaCV/L Conductivity 0
nhsCm) l3/ /OIZ. /821' Temperature 7..jqt-Z -Z .2-. '. q PH (S.U.) P.2C 6.0 64.) .ZO 1000 mg NaCIIL Conductivity (Wnhos/cm) zzq Z13 2 -73, Temperature -Z.Lq q,( ~ q 5? 7-44(2 -2 2q.2 Zj(
DO(g* 6.3 el 82
- 1200 mg NaCIJL Conductivity zI 244 (Muhos/cm) * ~ .to24+
Temperature ~S 1 '. '.~2(I PH (S.U.) .Z. .I~t ~I 6 1400 mg NaCIIL Conductivity (junhos/cm) 2q 16q, ~ s Temperature 7-q.2- 2q.S 2q.2-Z-(
initial ii Final II initial Imit iaal II Final Fin I initial Initi II Final H Final I Initial initial ii I
Final Final Initi
.54vciL /1/O/oo II ZZ00 IiU-oo
/I1L),50 //11/00 I0/o
Environmental 7Tsting Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 2.0 II I I I I i I I I I I I iI I I Warning and Control Limits Set According to 75th and 9 0 th Percentile CVs.
1.5 I ..................................................................................................
1.0 UV Treated Tests U'-
0.5 0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.11) is less than the 75 t and 90t percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
I I I I II I, Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC25)
.. Warning Limits (mean IC25 +/- SA.75, SA. 75 : 0.38, as determined by USEPA for the method and endpoint)
......... Control Limits (mean IC25 +/- S A.0 0.45, as determined by USEPA for the method and endpoint)
- 4) Environmental Ttsting Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 I I I I I I I 1 I I I I I I I I I I I I ControlLimits Set According to +/- 2 StandardDeviations.
1.0 I-S0.8 ............
°.° ". . . . ..
. .° - -.......... . . .................... ° .
°.. . . . . .. . . . . . . .* . . . . . . . . . . . . . . . . . .
UV Treated Tests in Q 0.6 0.4 k 0.2 I I I I I I I I I I I I I I I I I a? 04 ? O p 2 9 5 /
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
-- - Central Tendency (mean IC25)
......... Control Limits (mean IC25 + 2 Standard Deviations)
2,t Environmental ,
sting Solutions, LLC I)
Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water I I I I I I I I I I I I I 40K 30 UV Treated Tests 10 0 I I I I I I I I I I I I I I I I I I I I elk 4- a,? ! O 0q ý O'k 0-0 1ý 41 O4, 01- a,. a? 0 O Test date
-.- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
Central Tendency (mean PMSD)
......... Lower and Upper PMSD Bounds Lower PMSD Bound (10I' percentile) = 9.4%, Upper PMSD Bound (90'h percentile) = 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
A onmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number:
Dilution preparationinformation: Comments:
KC1 CCM number: C4 "0 U.'0.4 Stock preparation: 50 g KC/L: Dissolve 50 g KCI in 1-L
___ __ __ ___ __ __ Deionized water ___ ___ __ ___ __ ___ __
Dilution prep (mg/L) I 300 450 1 600 1 750 1 900 4-__ __-__ _ __,__ __ _ _ *,' "-'u *- , ,.. ' * -
Stock volume (mL)
Diluent volume (mL) 1 6 994 9
991 12 988 15 985 18 982 C)
I Test organism in ormation: Test information:
Organism age: Zq"-fkes OiL Randomizing template: t l7 Date and times organisms O-.0 t6 1, !Ib 7 ToI I -ý" Incubator number: z were born between: I Organism source: A& BF¶A 05-06-O2-- Artemia lot number: 7O1 Transfer bowl information: pH = Temperature = Oven temperature: (.0 C "7"140__ _ _Ž 0'c Drying time:
Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 1_ _-_
_ JO N A.) L 2 65-o?-oa- tow A67 i '?/0 3 O F~z-0 -OL 0 %1 1,:6 ao:, . t s oI j 4 FI Control information: Acceptance criteria Summary of test endpoints:
%Mortality: 2 20% 7-day LCSO '717,1 Average weight per larvae: 1 q%( Z
>0.25mg/larvae NOEC QI LOEC (too 1IC25 ýa.
46_ _ _1. _
ChV
Page 2 ot 5 knvironmental Testing Solutions, LLC PpKCICR Test Number: -W
- x- "te~eareb Yurvival and Growth Data 300M KCIIL 450 M KCI/L Day CONTROL E IF G H I J_ IJoK L A B C D
/ I() 10 (C) /0 /0 /0 10 0 /a /0 /0 /0 (0 /0 /0 /0 Io 10 16 10 1 /0 /0 10 /t lo e
/0 IC) 10 /0 2 10 !0 to /0 io /0 /0 to (0 3 IV IC) I() I z /0 10 16 4 /0 /0 /0 /0 /0 / c' 5 /0 /0 /0 /0 IC) /6 C /0
/0/6 / 1
.i 6 a~i,/0 /0
/0to /o q J( ti Ir A r==7 L7 Lo /0 /0 CW go)
A--Pn weight (-)
B - Pan + Larvae wedght (mg) 1
.ýpeD It.4 Larvae weight (mg) = A - B Calculations and data reviewed:
I
Page 3 ot 5 Erviroumental Testing Solutions, LLC PpKCICR Test Number: _Ži Survival and Growth Data 60 750 m2 KCI/L go mg -
Day M -1 "--
N 0 --" q "I
--- "R -V S* T W 6 I0 /0 0 1c)0 0 1()/I0 /0 4
/0 O /0 I A/ o / ,A1 8 Z 5 and data reviewed:
S~Calculations Comments:----
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR # 2, UV Treated Test dates: May 7-14, 2002 Reveiwed by: .& t Concentrutioo (mg/L Repilcate IniRtaln iber of Fial numbe of A- Pan weight B- Pan + Lare Larvae weight (mg) Wetight/Ilninhoaber Mean mrvval Mean weight (mg) tlient of Pent reucton from Ka) tame trvae (m0) weight(ag) - A- B oflarv (rag) (%) variation (V1 ontral (.)
A 10 9 14.545 19.310 4.765 0.4765 Control B 10 10 14.633 20.020 5.387 0.5387 97.5 0.4966 6.0 Not applicable C 10 10 14.867 19.840 4.973 0.4973 D 10 10 14.903 19.640 4.737 0.4737 E 10 10 14.720 19.880 5.160 0.5160 300 F 10 10 15.036 19.500 4.464 0.4464 97.5 0.4360 15.8 12.2 G 10 10 14.664 19.000 4.336 0.4336 H 10 9 15.059 18.540 3.481 0.3481 1 10 10 14.722 18.690 3.968 0.3968 450 1 10 10 14.851 18.860 4.009 0.4009 97.5 0.4120 6.6 17.0 K 10 10 14.791 18.770 3.979 0.3979 L 10 9 15.246 19.770 4.524 0.4524 M 10 9 14.713 17.840 3.127 0.3127 600 N 10 9 15.022 18.820 3.798 0.3798 95.0 0.3435 8.4 30.8 0 10 10 15.022 18.530 3.508 0.3508 P 10 10 14.692 18.000 3.308 0.3308 Q 10 6 15.065 18.440 3.375 0.3375 750 R 10 6 14.810 16.990 2.180 0.2180 52.5 0.2293 34.7 53.8 S 10 4 14.608 16.070 1.462 0.1462 T 10 5 14.607 16.760 2.153 0.2153 U 10 1 15.085 15.630 0.545 0.0545 900 V 10 4 14.916 16.460 1.544 0.1544 30.0 0.1210 37.9 75.6 W 10 4 14.817 16.300 1.483 0.1483 X 1 10 3 14.712 15.980 1.268 0.1268 Dunnett's MSD value: 0.087 MSD = Minimum Significant Difference PMSD: 17.5 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions. LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19.7% from the control (detnenined Lower PMSD bound determined by USEPA (10th percentile) 9.4%.
Upper PMSD bound determined by USEPA (90th percentile) = 35%.
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pirmephales growth in chronic reference toxicant tests-USEPA. 2000. Understanding and'Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
Organisms obtainedfrom Aquatic BioSyrtems. Inc 05-07-02UV.xls
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survval PpKCICR Sample ID: REF-Re"Toxicant. UV-tUeamd Start Damt 5n7)82 Test 11):
ETS-Env. Testing Solutions Sample Type: KCL-Pot,*ssut chloride End Date: 5/14/02 Lab ID.
EPAF91-EPA Freshwater Test Species: PP.Pimephabe promelas SamWle Da1. Protocol:
Coonamts:
ConoaW1 I 2 3 4 DContrl 0.9000 1.0000 1.0000 1.0000 300 1.0000 1.0000 LM 0.9000 450 1.0000 1.0000 ].0000 0.9000 600 0.9000 0.9000 1.0000 1.0000 750 0.6000 0.6000 0.4000 0.5000 900 0.1000 0.4000 0.400 0.3000 Number Total Transform: Arqan Square Root Rank 1-TaUed Re4p Number Mean Min Max CV% N Sumn Critical 1 40 Conc-*mg/L Mean N-Mean 1.3713 1.2490 1.4120 5.942 4 1 40 D-Costrol 0.9750 1.0000 1.3713 1.2490 1.4120 5.942 4 18.00 10.00 I 40 300 0.9750 1.0000 L.3713 1.2490 1.4120 5.942 4 10.00 10.0 2 40 450 0.9750 1.0000 1.2490 1.4120 7.072 4 16.00 10.00 19 40 600 0.9500 0.9744 1.3305 0.6847 0.8061 11.092 4 10.00 10.00 20 40
- 750 0.5250 0.5395 0.8106 10.00 10.00 "900 0.3000 0.3077 0.5677 0.3210 0.6847 30.172 4 Critical Skew Kurt Statistc Austihuy Teab -1.017845934 0.379250916 0.862000060 0.084 Shapi.o-Wilk's Test indicaitz non-noemal disn'bution (p - 0.01) 2.776863813 15.08631706 Bas.tdt Test indicates equal variances (p - 0.73)
NOEC LOEC ChV TU Hypothesis Test (I-lal, 05)
Steers Many-One Rank Test 600 750 670.8203932 Llkeilhood-Probil ChlSq Critical P-value Mu Sigma Iter CentreL par*meter Value SE 95% FIducal LImits 2.424613033 7.814724922 0.49 2.901507795 0.082578151 5 0.025 Slope 12.10974068 2.331844041 7.539326274 16.68015509 lelesept -30.13650687 6.745375227 -43.35744257 .16.915571M7 TSCR 0.022501990 0.013S0M721-0.004565055 0.049569051 1.0 Point Preolst mg/L 95% Fiduclal Limits 0.9.
ECOi 2.674 512.1560149 391.703859 5808151195 o.8 EC05 3.355 583.0137711 480.703891 640,2751492 0.7 EC]O 3.710 624.7116821 535.4309698 675.318892 3.964 654.5171571 575.2343058 700.768M006 0 0.6 EC15 EC20 4.158 679.2165533 608.3610921 722.3995151
- 0.5.
EC25 4.326 701.1479503 637.6098112 742.2855737 801.1398016 0.4" 0.5 EC40 4.747 759.6033144 712.0742551 ECS0 5.000 797.0907819 754.4481002 W46.0502775 0.3 EC60 5.253 836.428315 793.355763 900.2217852 0.2 EC75 5.674 906.1621171 852.5340065 1009.74882 5.042 935.4214269 875.2090525 1059.256191 0.1 EC80 ECas 6,036 970.7212707 901.5749537 1121.062246 0.0 EC90 6.282 1017.03508 935.0066861 1205.102395 10 100 1000 10000 EC95 6-645 1089.774769 905.6862065 1342.975512 Dose mgIL EC99 7.326 1240.547294 1086.116912 1648.841103 Dote-Response Plot 0.8 0.7 0.6 0 0.5 p'0.4 1--
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Sarvival Test-7 Day Growth Ppt(CICIS Sample ED: REF-Ref Toxicant. UV-trtated Sf1/02 Teat ID.
Start Daw Test ID: PpKCICR KCL-Potassiun chloride Lab ID: ETS-Ena. Testing Solutions Sample Type:
EndDa 5/14102 Protocol: EPAF 91 -EPA Freshwater Test Species: PP-Pimephales promodas Sampe Datw Ceeao9gL 1 2 3 4 D-Conrol 0.4765 0.5387 0.4973 0.4737 300 0.5160 0.4464 0.4336 0.3481 450 0.3968 0.4009 0.3979 0.4524 600 0.3127 0.3798 0.3508 0.3308 750 0.3375 0.2180 0.1462 0.2153 900 0.0545 0.1544 0.1483 0.1268 I.Ta~led Isotonic Transform: Untransformed N t-Stat Critical MSD Mean N-Mean N-Ma Mean Min Max CV%
Cow0-ng2L Mean 0.4966 1.0000 1.0000 0.4966 0.4737 0.5387 6.043 4 0-Conbrol 0.4966 8.4360 0.8781 15.801 4 1.678 2.410 0.0870 300 0.4360 0.8781 0-4360 0.3481 0.5160 2.343 2.410 0.0870 0.4120 0.8297 0.4120 0.3968 0.4524 6.551 4 450 0.4120 0.8297 0.3435 0.6918 8.371 4 4.241 2.410 0.0870 0.3435 0.6918 0.3435 0.3127 0.3798
-600 2.410 0.0870 0.2293 0.4617 0.1462 0.3375 34.656 4 7.409
-750 0.2293 0.4617 0.2293 0.1210 0.2437 0.1544 37.923 4 10.409 2.410 0.0870
.900 0.1210 0.2437 0.1210 0.0545 Critical Skew Kurt Statirtie Auxilinsry Tests 0.884 0.215608854 0.863392203 0.952484667 Shapim-Wilk's Tet indicates normal distribtuion (p > 0.01) 15.08631706 5.864162445 Bartletfs Teat indicates equalvarinces (p = 0.32)
ChV TU MSDU MSDp MSB MSE F-Prob df Hypothesas Tea (I-tall, M.85) NOEC LOEC 5.18 0.086950432 0.175109118 0.07932099 0.002603391 3.5E-08 450 600 519.6152423 Dunnetes Test Linear Interpolation (80 Resamples) peint Mg/L SD 95% CIAxp) Skew IC05* 123.06 97.92 32.63 484.41 1.0553 246.12 92.86 65.26 520.64 0.4150 ICI0*
92.53 87.11 536.37 -0.2871 1.0 ICIS 387.14 64.69 136.47 577.07 -2.0787 0.9 IC20 482.33 536.72 29.90 446.28 627.33 0.2279 IC25 29.85 610.87 812.87 1.4573 0 IC40 659.85 33.50 648.90 836.91 0.4917 0.7 IC50 725.03 n indicates IC estimate les than the lowest oancentration 200 400 600 800 1000 Dose mg/L D~ose-ResponsePlot 0.6.
0.5. Plot Dose-Respoa*
1-tail, 0.05 level of significance 0 0.3 P- 0.2 0.1 01
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CS-okM o&-',-o o.
pH (s.u.) , . . .z DO (mg/L)
Conductivity 2(
(n*,hos/cm) ________
Alkalinity (mg CaCO 3IL) _____
Hardness (mg CaCO 3iL) ____ _ _ _ _.
I Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the
,, _ _ _ _ acceptable range)
CONTROL 280 - 360 300 mg KCI/L 840 - 890 450 mg KCI/L 1100- 1180 600 mg KCI/L 1390 - 1460 750 mg KCI/L 1660- 1710 900 mg KCI/L 1890 - 1950 KCI Stock 67000 - 76000 (25 g KCI/L)
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DO (mg.2 -4.
Conductivity (AMImOS/CM) 305 z 310 .o Temperature zz q( '. qq Zbo Zq.3' 7q.
pH (S.U.) 6.Z 3 e6 911 DO (mg/L). (o.0 8-.
4500mg KCIIL Conductivity q Temperature z.10 2q~.3 L, A'LG/. 2q* VI pH (S.U.) jqZ. 86 A.1 -41 .. I&?
I DO (mgi'L) ell ~-1 450Omg KCIJL Conductivity I
Temperature -. 44 i.3 AS4 q.qL 7'/*
I 60gKILDO pH (S.U.)
(mg/L) q (3
.5
.- 1-q0
.0bj 1~
I 900mgKCILConductivity (Imos/cm Temperature 13 41 135~1 ff Z.O 13 Z 1 1 9 j5 'B i'ila 2qUI.h- 24, L'IU (0 Ifllfl2 (0c 24.
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