ML020500479
| ML020500479 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 01/16/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Haskin E Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML020500479 (71) | |
Text
Tennessee Valley Authority, Post Office Box 2000. Scz..-c.as' -ennessee 37379-2000 January 16, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT (SQN)
Please find enclosed the follow-up fourth quarter biomonitoring Discharge Monitoring Report (DMR) of January 2002 for SQN. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant
Enclosures:
2 (original & copy) cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control "State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555
PERMITTEE NAME/ADDRESS (include Faclilty Name/Location If Differentt amoe TVA-SEQUOYAH NUCLEAR PLANT A d*.ess
_0OQ2(_O00------------
-QD--
jlIILAIS-.2--j-31-------- --
ATTN: Dledre B. NIda NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
DISCHARGE MONITORING REPORT (DMR) t TN0026450 II101 T
PERIIII DISCHARGE NUMBER]
MONITORING pFRInD IFAR T
DAY I YFAR I MO I -)AY From o
o oIi To 02 0331]
MAJOR Form Approved.
(SUBR 01)
OMB No. 2040-0004 F - FINAL DIFFUSER DISCHARGE EFFLUENT NO DISCHARGE NOTE: Read instructions before compleiinq lhis form.
NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this documenet and all attachments were prepared under my
../2,,'
TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified Xe Richard T. Purcell personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering Environmental Supervisor the information, the information submitted is, to the best of my knowledge and belief, true, 423 843-6700 02 01 16 Site Vice President accurate, and complete. I am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE I
information, including the possibility of fine and imprisonment for knowing violations.
OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED
- con, COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here Repeat Fathead Minnow Test for fourth quarter 2001 was sampled from 1/2/2002 to 1/7/2002. Report is attached.
Pncnl_1nf 1' EPA Form 3320-1 (REV 3199)
Previous editions may be used I -
I --.
C A NADI C Parle 1 of 1,
January 15, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO.
TN0026450, REPAET FATHEAD MINNOW TEST, JANUARY, 2002 Attached are two copies of the subject report for submission to the state of Tennessee. The report provides results of compliance testing using fathead minnows. UV treated Outfall 101 samples were tested during this study, in addition to routine (non-treated) samples, to help determine the cause (eliminate the effect) of the low statistical sensitivity and highly variable survival among replicates within treatments that occurred in the study conducted with Outfall 101 samples collected December 9-14, 2001. Each UV Treated concentration was placed in a 40 WATT Rainbow Lifeguard UV Sterilizer for 1 minute to remove possible pathogenic interferences. I have also included a copy of the report for your records.
Side by side comparison of untreated Outfall 101 samples and ultra violet light (UV) treated Outfall 101 samples showed no toxic effects to fathead minnows in either test. The resulting IC, 5 values, for both tests, were > 100 percent.
Fathead minnow survival and growth in untreated and UV treated intake samples were not significantly different from the control, based on Homoscedastic t-Tests.
Call me at (256) 386-2755 if you have any questions or comments following your review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE
SUMMARY
- 1) Facility/Discharger: Sequovah Nuclear PlInt/TVA Report Date: January 15. 2002
- 2)
County/State: Hamilton/Tennessee
- 3) NPDES Permit #: TN0026450
- 4)
Type of Facility: Nuclear-fueled electric generating plant
- 5)
Design Flow (MGD): 3,266
- 6)
Receiving Stream: Tennessee River (TRM 483.6)
- 7) 1Q20: 2.992.4
- 8) Outfall Tested: 101
- 9) Dates Sampled: January 2-7. 2002
- 10) Flow on day(s) sampled (MGD): 1600, 1606, 1598, 1602, 1604. 1600
- 11) Pertinent site conditions: No unusual conditions reported.*
- 12) Test Dates: January 4-11. 2002
- 13) Test Type: Short-term Chronic-definitive
- 14) Test Species: Fathead Minnows (Pimephales promelas)
- 15) Concentrations Tested (%): Untreated Outfall 101: 10.98. 22.0. 43.9. 72.0. 100.0 Untreated Intake: 100.0 UV Treated Outfall 101: 10.98, 22.0. 43.9. 72.0. 100.0 UV Treated Intake: 100.0
- 16) Permit Limit Endpoint (%): Outfall 101 IC-?= 43.9
- 17) Test Results (%):
Untreated Outfall 101: Pimephales '/roneias.. ICi > 100 UV Treated Outfall 101: Pimephales promelas: IC, > 100
- 18) Facility
Contact:
Diedre B. Nida
- 19) Phone #: (423') 843-6700
- 20) Testing Lab Name: Environmental Testing Solutions, LLC
- 21) Lab
Contact:
Jim Sumner
- 22) Phone #: (828) 862-8193 TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- Production/operation data will be provided upon request or in the event toxicity exceeds permit limits
- 23) Notes:
UV treated Outfall 101 samples were tested during this study, in addition to routine (non treated) samples, to help determine the cause (eliminate the effect) of the low statistical sensitivity and highly variable survival among replicates within treatments that occurred in the study conducted with Outfall 101 samples collected December 9-14, 2001. Each UV Treated concentration was placed in a 40 WATT Rainbow Lifeguard UV Sterilizer for 1 minute to remove possible pathogenic interferences.
Untreated and UV treated samples collected from Outfall 101 exhibited no toxicity to P.
promelas based on the applicable permit limits, IC25 = 43.9 percent. Resulting IC?5 values for both Untreated and UV treated effluent were > 100 percent. Fathead minnow survival and growth in Untreated and UV treated intake samples were not significantly different from the control, based on Homoscedastic t-Tests.
METHODS
SUMMARY
Samples
- 1) Sampling Point: Outfall 101
- 2) Sample Type: Composite
- 3) Sample Information:
Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp.
TRC*
Used (MM-DD/Time)
(MM-DD/Time)
(°C)
(mg/L)
(MM-DD/Time)
(EST)
-(EST)
(EST) t10 01/02-0918 to 01/040814 0.8
<0.10 01/04 1403 01/03 -0818 01/05 -1410 Intake 01/02-1013to 01/040814 0.6
<0.10
. 01/04 1403 01/03- 0913 01/05-1410 101 01/04- 0935 to 01/05 1336 0.4
<0.10 01/06-1400 01/05-0939 01/07 -1409 Intake 01/04-1032 to 01/05 1336 0.6
< 0.10 01/06 -1400 01/05- 0932 01/07 -1409 101 01/06-1328 to 01/08 0816 1.0
< 0.10 01/08 -1407 01/07-1234 01/09 -1431 01/10-1217 Intake 01/06-1351 to 01/08 0816 0.8
< 0.10 01/08 -1407 01/07-1251 01/09 -1431
-__ I I
-L 01/10 -1217
- Total residual chlorine.
- 4) Sample manipulation:
Untreated samples of Outfall 101 and Intake were warmed to test temperature (25.0 1.00C) in a warm water bath.
2
Test Organisms
- 1) Source:
- 2) Age:
Pimephales promelas Aquatic BioSystems. Inc.
23.5-25.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> Test Method Summary (See Appendix A for additional information)
Pimephales promelas
- 1) Test Conditions
- 2) Test Duration
- 3) Dilution/Control Water
- 4) Number Replicates
- 5) Animals -per Replicate
- 6) Test Initiation (Date/Time)
- 7) Test Termination (Date/Time)
- 8) Test Temperature Static. renewal 7 davs Moderately Hard Synthetic Water 4
10 01/04/02/1403 EST 01/11/02/1420 EST Mean = 24.6°C (24.0°C-25.50 C)
- 9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivitv were measured at the laboratory in each 100 percent sample. Daily temperatures were measured in one replication per test concentratioq..Pre-and post exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10) Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
3
TOXICITY TEST RESULTS (See Appendix C for Bench Sheets)
- 1) Results of a Pimephales promelas Chronic/ 7-d Toxicity Test (Genus)
(Species)
(Type/Duration)
Conducted 01/04/02 - 01/11/02 Using Effluent From Untreated Outfall 101 (mm/dd/yy) (mm/dd/yy)
(number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4
5 6
7 Control 100 98 98 98 98 98 98 10.98% Effluent 100 95 95 95 95 95 85 22.0% Effluent 100 100 100 98 98 98 98 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 O00 100 Intake 100 100 100 100 100 100 100 IC2s Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
34 Mean Control 0.819 1.119 0.982 0.963 0.971 10.98% Effluent 1.067 0.959 0.329 1.066 0.855 22.0% Effluent 1.097 0.989 0.939 1.085 1.027 43.9% Effluent 0.962 1.004 0.983 1.018 0.991 72% Effluent 0.9077 0.946 0.822 1.018 0.941 100% Effluent 1.005 0.963 0.978 1.132 1.020 Intake 0.989 1.036 1.086 0.901.
1.003 IC25 Value > 100%
Calculated TU Estimates
<1.0 TUc 95% Confidence Limits Permit Limit 2.3 TUc Upper Limit = NA Lower Limit = NA
'NOTE: TUc = 100/ IC2*
4
- 2) Results of a Pimephales (Genus) promelas Chronic/ 7-d Toxicity Test (Species)
(Type/Duration)
Conducted 0 1/04/02 - 01/11/02 Using Effluent From UV Treated Outfall 101 (mm/dd/vy) (mm/dd/yy)
(number)
Percent Surviving Test Solutions (time intervals used - days) 1 2
3 4
-5 6
7 Control 100 100 100 100 100 100 100 10.98% Effluent 100 100 100 100 100 100 100 22.0% Effluent 100 0_0 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72% Effluent 100 95 95 95 95 95 95 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 IC25 Mean Dry Weight (mg)
Test Solutions (Replication) 1 2
3 4
Mean Control 1.089 1.041 0.987 1.161 1.070 10.98% Effluent 0.914 1.146 1.042 1.157 1.065 22.0% Effluent 0.927 1.003 0.994 1.043 0.992 43.9% Effluent 1.004 0.928 1.055 1.026 1.003 72% Effluent 0.685 1.058 1.000 1.022 0.941 100% Effluent 1.031 0.981 0.891 0.796 0.925 Intake 1.0039 0.838 0.956 0.980 0.946 IC,5 Value > 100%
Calculated TU Estimates T <1.0 TUc 95% Confidence Limits Permit Limit 2.3 TUc Upper Limit = NA Lower Limit = NA "NOTE: TUc = 100/ 1C25
- 3) REFERENCE TOXICANT TEST RESULTS (See Appendixes A and D)
Species Date Time Duration Toxicant Results (1C25)
Pimephalespromelas 01/04/02 1431 7-days KCI 487.8 ng/L 5
SUMMARY
/CONCLUSIONS Untreated and UV treated samples collected from Outfall 101 exhibited no toxicity to P. promelas
-based on the applicable permit limits. Resulting IC25 values for both Untreated and UV treated effluent were > 100 percent. Fathead minnow survival and growth in Untreated and UV treated intake samples was not significantly different from the control, based on Homoscedastpc t-Tests.
4
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms. per treatment. Test vessels consisted of 400-mL beakers, each containing 250 mL of test solution. [2]
DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL The third renewal using the last samples collected was conducted greater than 1 hour1.157407e-5 days <br />2.777778e-4 hours <br />1.653439e-6 weeks <br />3.805e-7 months <br /> from the test initiation time to meet the required 72-hour hold time.
DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS The concurrent reference toxicant test conducted on this batch of fish was just below control chart IC,- limits. The resulting endpoint was 0.015 mg lower than !he -2SD lower boundary.
Two SD allowed a reduction of 22.6 percent from controls, while the reference toxicant results were reduced 24.9 percent from controls. Previous reference toxicant tests conducted within 30 days by both ETS and the culture lab were within bounds and this was the first outlier in 18 tests ETS has performed (one outlier in twenty is expected). If animals were more sensitive, effects would have been more likely in the effluent tests and both effluent tests clearly showed no toxicity. Both tests far surpassed both survival and growth test acceptability criteria.
PHYSICAL AND CHEMICAL METHODS
- 1) Reagents. Titrants, Buffers. etc.: All chemicals were certified products ýsed before expiration dates (where applicable).
- 2) Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3) Temperature was measured using EPA Method 170.1.
- 5) The pH was measured using EPA Method 150.1.
- 6) Conductance was measured using EPA Method 120.1.
- 7) Alkalinity was measured using EPA Method 310.1.
- 8) Hardness was measured using EPA Method 130.2.
7
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage; glassware preparation; test organism culturing/acquisition and acclimation; test organism handling during test; and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. [2] Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix D for control chart information)
- 1) Test Type: 7-day chronic tests with results expressed as IC25 values in g KCI/L.
- 3) Dilution Water Used: Moderately hard synthetic water.
- 4) Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
REFERENCES I) NPDES Permit No. TN0026450.
- 2) Lewis. P. A.. D. J. Klemm. J. M. Lazorchak. T. J. Norbera-King. W. H. Peltier, M. A. Heber. Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Oraanisms. EPA/600/4-91/002 (July 1994).
- 3) Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
8
Sequoyah Nuclear Plant Biomonitoring January 4-11, 2001 Appendix B Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests I
'i
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, -During Toxicity Test Sampling, March 12, 1998-January 7, 2002 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M hypochlorite PF mg/L TRC mg/L TRC mg/L mg/L mgiL mg/L Azole mg/L Phosphate Copolymer DMAD Quat 03/12/1998 0.016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/1 1/1998 0.013 0.055 0.019 09/12/1998
< 0.001 0.044 0.015 09/13/1998
< 0.001 0.044 0.015 09/14/1998 0.008 0.044 0.015 02,.22/1999
< 0.001 02123/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999
< 0.001 02/28/1999
< 0.001 I
I 08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070 0.024 0.120 08/21/1999 0.022 0.068 0.024
- I 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025
'0.068 0.024 0.006 08/24/1999 0.016 0.067 0.023 0.020
-S,
S
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-January 7, 2002 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M hypochlorite PF mg/L TRC mg/L TRC mg/L mg/L mgJL mg/L Azole mg/L I
Phosphate Copolymer DMAD Quat 01/31/2000 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 0.002 0.027 0.009 02/06/2000
< 0.002 0.027 0.009 07/26/2000 0.0057 0.055 0.019 0.021 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000
< 0.0088 0.055 0.019 07/30/2000
< 0.0076 0.055 0.019 07/31/2000
<0.0152 0.055 0.019 0.006 08/01/2000
< 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12112'2000 0.0092 0.025 0.020 0.005 12/13/2000 0.0120 0.025 0,020 12/14/2000 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 0.0036 0.025 0.020 12/17/2000
< 0.0036 0.025 0.020 08/26/2001 0.017 0.06 0.021 0.006 08/27/2001
<0.0096 0.06 0.021 0.005 0.021 08/28/2001
<0.0085 0.06 0.021 08/29/2001
<0.0094 0.059 0.020 0.00ý.
0.021 08/30/2001
<0.0123 0.06 0.021 0.005 08/31/2001
<0.005 1"x0.059 0.020 11/25/2001
<0.0044 I 1/26/2001
<0.0119 0.024 0.02 0.005 1 /27/2001 0.0137 0.023 0.019 0.007 11/28/2001
<0.0089 0.022 0.019 0.006 11/29/2001 0.0 132 0.024 0.02 0.007 1I1/30/2001 0.0043 0.024 0.02 8
Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-January 7, 2002 Date i
Towerbrom
?iL PCL-401 CL.-6$.Cuprostat i
mg/LTRC i mg/L i[ JL mg/L i
Thiii Copolymer tiMA Azole 12/09/200 1
<0.0042 12/10/2001
<0.0042 12/11/2001
<0.0104 12/12/2001 0.0128
~
I.0.02 12/13/2001
<0.0088 0.02 12/14/2001 00134 002
&Oi7 01/03/02
<0.0042 OOiliii~*
- ii~6 !~
0.014
-ii:iiii:ii:!!iii::ii 01/04/02 0.0124 iLH~liii:**4.iiii!~
0.014
- ii iiii 01/06/02
<00042 01/07/02
<0.0089 42 l24.
0014 "12
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Fathead Minnow Test, Sequoyah Nuclear Plant Effluent (SQN), Untreated Outfall i01, January 4-I I, 2002 Test/
Tenleralure Dissolved Oxygn!!
R i1 Conductance Alkalinity Ilardness Total Residual Sample Initial Final Initial Final Initial Final Initial Chlorine ID (0C)
(0c (ig/I)
(mg/I.)
S.I.J.
S.IJ.
(plmhos) nig/L CaCO 3 nmg/l, CaCO, (mg/L)
Fathead/
24.8 24.4 8.0 7.5 8.06 7.75 308 67 90 Control (24.4-25.5)
(24.1-24.8)
(8.0-8.1)
(7,0-7.8)
(8.01-8. 10)
(7.62-7.84)
(300i-320)
(65-68)
(90-90)
Fathead/
24.8 24.4 8.0 7.4 8.07 7.73 300 10.98%
(24.4-25.4)
(24.2-24.8)
(7.9-8.1)
(7.1-7.9)
(8.03-8.11)
(7.60-7.79)
(290-315)
Fatheady...
24.8 24.4 8.!
7.4 8.06 7.72 290 22.0 % ",
(24.4-25.4)
(24.1-24.6)
(7.9-8.2)
(7.1-7.9)
(8.02-8.10)
(7.57-7.86)
(281-304)
Fathead/ \\
24.8 24.4 8.2 7.4 8.02 7.69 265 43.9%
,(24.4-25.4)
(24.1-24.7)
(8.0-8.4)
(7.1-7.9)
(7.92-8.08)
(7.55-7.82)
(257-277)
Fathead/
24.8 24.4 8.3 7.4 7.99 7.69 232 72.0%
(24.4-25.4)
(24.1-24.8)
(8.0-8.5)
(7.0-7.8)
(7.93-8.06)
(7.54-7.79)
(225-242)
Fathead/
24.8 24.4 8.4 7.4 7.93 7.66 197 65' 77
<0. 10 100%
(24.4-25.4)
(24.1-24.8)
(8.1-8.6)
(7.0-7.9)
(7.85-8.05)
(7.53-7.81)
(192-207)
(61-69)
(74-80)
(<0.I0-<O.10)
Fathead/
24.8 24.4 8.4 7.4 7.87 7.74 196 64 78
<0.10 Intake (24.4-25.4)
(24.1-24.8)
(8.1-8.6)
(7.0-7.9)
(7.76-8.03)
(7.61-7.83)
(192-206)
(62-65)
(74-80)
(<0.I0-<0.10)
PHYSICAL/CHEMICAL
SUMMARY
(continued)
Water Chemistry Mean Values and Ranges for Fathead Minnow Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, January 4-1I, 2002 Test/
Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Total Residual Sample Initial Final Initial Final Initial Final Initial Chlorine ID
(°C)
(0C)
(rng/L)
(mg/L)
S.U.
S.U.
r(nmhos) rg/L CaCO3 rng/L CaCO3 (rag/L)
Fathead/
24.8 24.4 8.0 7.5 7.92 7.76 307 67 90 Control (24.4-25.4)
(24.2-24.7)
(7.9-8.1)
(7.2-7.8)
(7.66-8.04)
(7.68-7.88)
(296-321)
(65-68)
(90-90)
Fathead/
24.8 24.4
- 8. I 7.6 7.97 7.77 298 10.98%
(24.4-25.4)
(24.2-24.7)
(7.9-8.2)
(7.2-7.8)
(7.81-8.03)
(7.68-7.87)
(289-318)
Fathead/. i 24.8 24.4 8.2 7.6 8.00 7.74 287 22.0 %,
(24.4-25.4)
(24.0-24.7)
(8.0-8.3)
(7.3-7.8)
(7.93-8.06)
(7.62-7.81)
(278-305)
Fathead/ \\
24.8 24.4 8.3 7.6
.8.02 7.75 263 43.9 %
.(24.4-25.4)
(24.1-24.7)
(8.1-8.4)
(7.2-7.9)
(7.96-8.06)
(7.64-7.83)
(256-277)
Fathead/
24.8 24.4 8.3 7.6 8.01 7.72 228 72.0%
(24.4-25.4)
(24.1-24.7)
(8.1-8.5)-.
(7.4-7.9)
(7.96-8.06)
(7.60-7.83)
(220-244)
Fathead/
24.8 24.4 8.4 7.6 7.99 7.72 196 65 77
<0.10 100.0%
(24.4-25.4)
(24.1-24.7)
(8.1-8.6)
(7.3-7.9)
(7.95-8.07)
(7.59-7T81)
(189-207)
(61-69)
(74-80)
(<0. I 0-<0.10)
Fathead/
24.8 24.4 8.4 7.6 7.97 7.71 194 64 78
<0.10 Intake (24.4-25.4)
(24.1-24.7)
(8.1-8.7)
(7.3-7.8)
(7.91-8.05)
(7.62-7.82)
(189-207)
(62-65)
(74-80)
(<0.I0-<0.10)
.1
Sequoyah Nuclear Plant Biomonitoring January 4 - 11, 2002 Appendix C Chain of Custody Records and Toxicity Bench Sheets
- o. I.
BIOMONITORING CHAIN OF CUSTODY RECORD
'Page 1
of
ý1 Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: P0 BOX 2000 1 Ecusta Road, Brevard, NC 28712 P.O.Numb r:
P BOX 2000Other (specify):
-M AX COULTER Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN Sample Cust-ody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time nstructions: Clients should rll in all areas except those in the
-'abonitoty Use" block. l3iononitoring Sales)leS are p)reserved by storing them a*t 4°C and shipping themn in ice. The hold time for each ample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therelbre, please collect and ship in such a way theat the laboratory will receive the samples with ample time to initiate testing within that time frame.
- amples shipped overnight on Friday via FedEx or IJPS must be marked lor Saturday delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN (CITrnr,
nv
Client: TVA Project Name: TVA SQN P.O. Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: 0026450 Collected By: WANDA ALLEN 1
IMNT CAN RISO Environmental Testing Solutions, LLC Research Building I Ecusta Road, Brevard, NC 28712 Phone: 828-862-8193 Fax: 828-862-8195 I
I I
I Delivered By (Circle One):
FedEx UPS Bus Page of (I
Other (specify):
MAX COULTER General Colnillents:
istructions: Clients should fill in all areas except those in the "Laboratory Use" block. BioLlonitoring samples are preserved by storing them at 4*C and shipping them in ice. The hold time for each Imple is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame, Amples shipped overnight onl Friday via FedE-x or UPS mu~st be marked for Saltirday delivery or they will not arrive until the fiollowing Monday.
Client
Container Field Identification /
Grab/
Ship.
Collection Date/Time Number &
Flow Rain Event?
C l e t o U.::=i i i~
Sample De~criltion Comp.
Temp. (
Volume (MGI))
(Mark as Appropriate)
- se Collected Start EndIf
- Yes, No Trace EST to*
Aitiva TB A:
d Inclies N
i'
[
er SQN-1Ol-TOP' comp 4
1/6/02 2 (2.5g)
SQN-INT-TOX comp cop 4
6ý
)I (2.5g)
Olp
- . j.........
Sample Custody - Fill In From Top Down Relinquished By (Signature):
Date/Time Received By (Signature):
Date/Time nstructions: Clients should fill in all areas except those in the
-'Laboratory Use" block. Biolnonitoring samples are preserved by storing them at 4VC and shipping them in ice. The hold time for each ample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame.
- amples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the lbllowing Monday.
t
EtnvironmentalI ieizaig Soutu xuI, Sample Receipt Log Date Time Received Received Sample Project Sample Received Received By From Temperature Number Number Sample Name and Description State Comments 12-13-01 1zqc)
Kik-ee4awh#
Feb
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1300 Blue Spruce Drive, Suite C Fort Collins, Colorado 80524 Toll Free: 800/331-5916 Tel: 970/484-5091 Fax:970/484-2514 ORGANISM HISTORY SPECIES:
AGE:
LIFE STAGE:
HATCH DATE:
BEGAN FEEDING:
FOOD:
Water Chemistry Record:
TEMPERATURE:
SALINITY/CONDUCTIVITY:
TOTAL HARDNESS (as CaCO3):
TOTAL ALKALINITY (as CaCO3):
pH:
Comments:
Pimephales promelas N/A Embryo 1/3/02 Between 11:30 a.m.&1:30 p.m.MST N/A N/A Mean 25 °C Range 124 mg/I 90 mg/1l 8.09 Tfoq0...l,
- q~t(
- 27o. -
Ao
~~~
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Facility Supervisor
- ~
Aquatic BioSystems, Inc Quality Research Organisms DATE:
1/3/02
Luvironmental Testing Solutions, LLC p3
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promelas Client: -'T-A V*u0"At14 cLe&
PLAT" F a c il it y :
. m (
- " o
-. re _e NPDES #: -r/qJ 662G3 (Q" Daily feeding and renewal information:
Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 2
S "Te rs
- w t
s* aGT veA e
T "i2 -Aou( "
fkKTe'ST imm~~i,,ao
-rtjee-12-AoufA AOLb TIW-
_l-affi ;eI of lO
JEnvironmenta1 Testing Solutions, LLC tL.
Species: Pimephales promelas Client:
IOL AoAU(
N&Ir PLtrr 0 Lrr ALL lot - kjot'ZAe Date:
0-04-07-.
Calculations and data reviewed:
r4gu z of 10 x'-4*
- OII U
Luvironmental Testing Solutions, LLC Species: Pimephales promelas Client: "T"A AALPNA/4 A
A iJr OUrP'AL 101 - AoN reb Date:
iol-6(4-O" Calculations and data reviewed: _
U-3 of 10
Environmental Testin Solutions, LLC Page 4 of 10 Species: Pimephales promelas Client:
"VA -
t i1*
- I-iA lr CFALL. 101-0 ome::ecr Date:
,1-6q-oZ..
- Day 0
1 2
3 4
5 6
7 A =Pan weight (-g)
B = Pan + Larvae weight (rg)
Lar.ae. weight (mg) = A - B Survival and Growth Dlata RB
/o
/0
/o
/0
/0 (0
/0
/o 10
/0
/0
/0 1()
/0 Io
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Calculations and data reviewed:
.t 4
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L.
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/
Environmental Testing Solutions, LLC 1 Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client:
TVA Sequoyah Nuclear Plant - Nontreated Teat dates:
January 4 -11, 2002 Project number'.
221 D
0 Fina 15.00 2463 of62 0.9629ih-(gB-Pn eF-ewMa sria larEae larva weight (mg) 10 I Itlr umer 25.680 co.n o
A variaton v%
control
()
M3.1ro 10 1.1 4.61 0.8187 Cotrl B10 90 14.973 23.140 118.187 118 10% V 10 10 14.956 24.780 9.682 0.98 97.5
.09 5 7.6
-5.0 ut 010 10 15.1 24.630 9.629 0.9629 10 15.0 13 2 5.6 80 10.6 67
- 1. 6622 10." %
F 10 10 1 4. 932 24.9 30
- 9. 588 0.9 598 G"
10 4
14.962 18.250 3.288 0.3288 U
41.5 11.9 100%ii 10 10 I4.820 25.340 10.660 1.066.
,':t*
i10 10 14.9 6 5 2 5.9 3 0 1. 6
. 9 5 22
- t'*"
1010 15.156 25.050 984099 75107
.'? K 10 9
14.'942 24.330 9.3880.38.4-.
n 10 10 14.910 25.760 10.850 M
10 10 14.949 24.570 9
0.9008 43,9%N 10 10145.
1 43.9%
10 10 15.015 24.840 9.825 0.982510.0914.421 P
10 10 14.925 251010.175 1.0175 9
10 10 14.950 24:.720 72%
R 10 10 15.130 24,590 940096 S
10 10 15,132 23.35A
.1
.2 810009 0
T 10 10 14.057 5.140 10
-"to-10 1--
.13_
100%
101 01
.4810 4
Oufl 0 10 10502 2.70968092 O utfal B0 R
- - -- 6 51 9
-, 24900 9.781 0.9781 100.0
!.0195 7.6
-5.0 10 10 14.948 26.2 7 0 11.3221.3 2
10
~
15.012 2 --
-i 9.888---"
0.98-88--
ioo%
- To 10 14'.995 25.350 1.5 IO5 Itk 110 0 o 15:162 26.020 10.858 1.0858 100.0 1.0027 7.8
-3.3
-1 0
I0 14.962 23.970 9.008 0.9008 Dunneti's MSD value:
PMSD:
Dunnett's MSD value:
PMSD:
0.2785 28.7 0.1417 14.6 Minimum Significant Difference PMSD Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19. 1% from the control (determined through reference toxicant testing).
Lower PMSD bound determined by USEPA (10th percentile) = 9.4%.
Upper PMSD bound determined by LtSEPA (90th percentile) = 35%,
The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.
snp 01-04-02.xls
,i
Environmental Testing Solutions, LLC Statistical Analyses Larma Flrt Grtxw and Survival Te--7 Oay Growr Sim Dam Tema Ill S ps IsD,-,
TVA SQN - Oq>X 101 Nobatued End Date I/1lm2 LtAb.
M W
E Ts-E.
Te S*as sok SamnpleTyp MLtisbnV Mowati ; Report SmplC Dam PMtoool EPAF 91-EPA Frewnu Teo SpecieP p
tpeanes commalePcp~me, Coruc-%
1 2
34 D-Coea, o 0.8187 1.1189,-
0.9824
- 6629 10.98 1.0667 0.9592 03288 1.0660 22 1.0965 0.9894 0.9398 1.0850 43.9 0.9621 1.0035 0.9825 1.0175 72 0.9770 0.9460 0.8218 1.0183 100 1.0048 0.9628 0.9781 1.1322 Traniform Unuansforeed Rank I-Tailed Isotonic Conic%
Mean N-Mean Mean M
C%
S Cti Mean N-Mean D-Con1.00 0.9707 0.8187 1.1189 12.654 4
0.9707 1.0000 10.98 0.8552 0.8810 0.8552 0.3288 1.0667 41.457 4
17.00 10.08 0.9669 0.9960 22 1.0274 1.0584 1.0274 0.9398 1.0965 7.410 4
20.00 10.00 0.9669 0.9960 43.9 0.9914 1.0213 0.9914 0.9621 1.0175 2.447 4
20.00 10.00 0.9669 0.9960 72 0.9408 0.9691 0.9408 0.8218 1.0183 9.000 4
17.00 10.00 0.9669 0.9960 100 1.0195 1.0502 1.0195 0.9628 1.1322 7.565 4
20.00 10.00 0.9669 0.9960 Aulellinry Terns StCritica Skew Kurt b~ale ks Teat ind aze ne-*
4(p.
On (p 0.01) 0.827690006 0s.84
-1.90286024 7.108732712 B-us Tetudue asul vaiunE 3
19.07797623 15.08631706 Hlypotesis Ts t(1-4a. 0.05")
NOEC LOEC OhV TU Std's Many-One Rank Tea 100
>100 I
Ustar Interpolation (80 Reumples)
Point SD 9"% CL*Exp)
Skew ICOS
>100 ICIO
>100 IC15
>100 1.0 IC20
>100 0.9 IC25
>100 1C40
>100 0.8 ICSO
>100 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0.0
-0.1
-0.2 0
20 40 60 80 100 120 Doese-t-
-sp e Plot 1.2
-en f IfIt.1 n1
Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation only.
Larval Fish Growth and Survival Test-7 Day Growth Start Date:
114/02 Test M1D.
PpFRCR Sample ED.
TVA SQN - Outfall OT Nonnea=ed End Da-,
1/111/02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
DMR-Discharge Monioring Report Sample Date:
Protocol:
EPAF 91-EPA Freshwater Test Species:
PP-Pimcphales promelas Comments:
Conc-%
1 2
3 4
D-Contron 0.8187 1.1189 0.9824 0.9629 10.98 1.0667 0.9592 0.3288 1.0660 22 1.0965 0.9894 0.9388 1.0850 43.9 0.9621 1.0035 0.9825 1.0175 72 0.9770 0.9460 0.8218 1.0183 100 1.0048 0.9628 0.9781 1.1322 Transform: Untransformed I-Tailed Lonc-%
Mean N-Mean Mean MNn Max CV%
N t-Stat Critical MSD D-Control 0.9707 1.0000 0.9707 0.8187 1.1189 12.654 4
10.98 0.8552 0.8810 0.8552 0.3288 1.0667 41.457 4
1.000 2.410 0.2785 22 1.0274 1.0584 1.0274 0.9388 1.0965
-7.410 4
-0.491 2.410 0.2785 43.9 0.9914 1.0213 0.9914 0.9621 1.0175 2.447 4
-0.179 2.410 0.2785 72 0.9408 0.9691 0.9408 0.8218 1.0183 9.000 4
0.259 2.410 0.2785 100 1.0195 1.0502 1.0195 0.9628 1.1322 7.565 4
a0.422 2.410 0.2785 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.0 )
0.827680886 0.884
-1L98286024 7.208732712 Barriers Test indicates unequal variances (p = 1.86E-03) 19.07797623 15.08631706 Hypothesis Test (1-tail. 0.05)
NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetrs Test 100
>100 1
0.278529016 0.286928858 0.016164118 0.026713868 0.696975112 5,18 Dose-Response Plot 1.2 0.8 0.6.
0.4.
0.2 UCO N
04 t4 1-tail, 0.05 level of significance 0
0 U I
.gnn OIDndD*
1Io
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growt and Survival Test-7 Day Growth Start Date:
1/4/02 Test I5.
PpPFCR Sample ID:
TVA SQN - Intake Nontreated End Date:
1/11/02 Lab ID-ETS-Ew. Testing Solutions Saw !e Type:
DUR-Discharge Monitoring Report Sample Date:
Protocol:
EPAF 91-EPA Freshwater Test Species:
PP-Pimephales pronelas Comments:
Conc-%
1 2
3 4
D-Contrl 0.8187 1.1189 0.9824 0.9629 100 0.9888 1.0355 1.0858 0.9008 Transform: Untransformed 1-Tailed aonc-%
Mean Mean MNn Max CV%
N t-Star Critical MSD D-Control 0.9707 1.0000 0.9707 0.8187 1.1189 12.654 4
100 1.0027 1.0330 1.0027 0.9008 1.0858 7.844 4
-0.439 1.943 0.1417 Auxiliary Statistic Critical Skew Kurt Shapindilke esa inces (pn = 0.4 o
0.974953771 0.749
-0.15580785
-0.06228885 F-Test indicates equal variances (p =0.48) 2.439072847 47.46834564 Hypothesis Test (1-tail 0.05)
Homoscedastic t T est indicates no significant differences Dose-Response Plot 1.2 0.8 a 0.6 0
" 0.4 0.2 0
00 8,(...
QI
".-1 v, rn f)lh -j d.. l _/
Lnvlronmental Testing Solutions, LLC Page I or Io Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species:
mephkaespromelas Client:
VA,Aa',/r A
POW Facility: (-J=ALL
,ol - f*r'eAT.*
NPDES #: T"4, of*l2ýq5 Daily feeding and renewal informatj Day I
Date mn;-
time ion:
Afternoon feeding Test initiation, renewal, Analyst time or termination time Jq 31#NO "12-Voo.
AOLh, TMe..
Lnvironmen~ tfesting Solutions, LLC Page 2 of 10 Species: Pimephalespromnelas Clent:
$Jc&G ot,
- e.
{.
U)Q -TVPA.e-t Day 2
3 4
5 6
7 A = Pan weight (mg)
B Pan + Larvae weight (rag)
Larvaewe ight (mg) = A - B Date:
ot -c*,4-z Survival and Growth Data CONTROL M.9 9\\7.
A!BC
.1o 6
/
jO /0
/
to
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Calculations and data reviewed:
-E I F I-G I H I
mnvironmental Testin Solutions, ULC Page 3 of to Species: Pimephales promelas Client:
ý-
0A 6
)AC týc IA*e.*&*A-,-
Date: n%-oq-c,07 Day 0
I 2
3 4
5 6
7 A = Pan weight (mg)
B Pan + Larvae weight (mrg)
Larvae weight (tug) = A - B M IN 10 4O
/0
/0
/
/0 I0) 10 Io (0
Ib-10
/DL 10 10
/c Ac 004/
1 4Lj.~L too fpf%
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Calculations and data reviewed:
10
/O 10
/0 to 10
/0
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/0
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4
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Environmental Testing Solutions, LLC Chronic Whole Effluent 'l'oxicity 'lest (E'PA/600/4-91/002, Method 1002.0)
Species: Pimephales promelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client:
TVA Sequoyah Nuclear Plant - UV Treated Test dates:
January 4 -11, 2002 Project number:
221by:
Pro Fct 10number 21 n surviva 26an w2 m
Coe.t0 1nt7ron lavat l
ara weight {rag)
- A-11 vaiaio lara contro
((%)
A t0 10 14.821 25.710 10.889 01.0889 0.1 Control 10 14.797 610413 1.0413 100.0 1.0697 6.9 Not 0.8c0l X
10 10 14.797 24.670 9.873 0.9873 D
10 I0 15.129 26.740 11.611 1.1611 0
E 100 10 15.005 24.140 9.135 0.9135
, 09%F I0 i10 14.938 26.400 11.462
- 1. 14692 100.0 1.0648 9.
'G 10 10 14.990 25.410 in 420 1
.42
- 10.
0.5 SH10 10 15.057 26.630 11!.573 1015 73
!10 10 1
14.833 24.100 9.267 0.;7267-
--,. i t
J 10 10 14-793 24.820 10.027 1.0027 100.0 0.9917 4.9 7.3 K
110 t0 14.987 24.930 9.943 0.9943 L
l L
10 10 14.810 25.240 10.430 1.0430 M
1..
0 10 14.813 24.850 10.037 r
t.0037estng) 43.9%
N ob0 10 14.794 wer5.0740 10.i6d U
10t6 100.0 1.0031 5.496 0
10 10 14.7942.300.4!.566.
-P 0
10 14.81-7 5.080
-I
.-2-6"30 1.0263 Q
10 8
15.121 21.970 6.849 0.6849 72%
R I 0 I10 14.896 25.480 1-0.584 1.0584 S
11014.892 24.890 9.998 0.9998 95.0 0.9412 18.3 12.0 T
10 10 14.912 25.1s 10.218 "-
1.0218 U
1010 14.807ý 25.120 10.313 1,0313 100%
V I10 10 15.090 4.0 9.810 0.9810 100094 Outfall 101 W10 014.865 8905 Y
10eto 15A151 25C240inc9nnati-100%
Z 10 10
- 14..7"9 2-10-.8 oRR I n t a k e A A t o 1 I0 1 4 ' 9 6 0 2 4. 2 9.5 6 0
.9 61 0 00 9 4 87
.9 1 1.6 10 1.940 2474 1.00 oT 0 9800-l
~
MSD =
Minimum Significant Difference Dounnett's MSD value:
0.1762 PMSD =
Percent Minimum Significant Difference PMSD:
16.5 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significantdifrneousfrEvrnmta Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 19. 1% from the control (determined Intake..
through reference toxicant testing).
Po n etMSD:
vaue 9.102 ower PMSD bound determined by USEPA (I10th percentile) = 9.4%,
PNISD 9.6Upper PMSD bound determined by USEPA (90th percentile) = 35%.
T1he lower and tipper hounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole l:ffluenft Toxicity Applications Under the National Pollutant Discharge Elimination Program. E-PA-833-R-00-003. US Environmental P ro te c t io n A g e n c y, C in c in n a t i, 0 1 1.
s p O - d O. l
Environmental Testing Solutions, LLC Statistical Analyses Larva] ftk Gowth And Survival Tes.7 Day Growth Stlan 1am
/40 Tat aD PpFRWA Smple D:
"T VA SQN - Oa1l. 101 UV Tr=a Fad D=ur 1/1 Ml2 L M, 1D 3 av. Tr a Soukm SImple TynC DMR-Dkad-Monnqig Report S=
DCamm Protocol EPAP O-EPAPT.:ww Tat Speia PP-Pinnea pate lasd Con*.%
2 3
4 D-Comni 1.0989 1.0413 0.987; 1.1611 10.98 0.9135 1.1462 1.0420 1.1573 22 0.9267 1.0027 0.9943 1.0430 43.9 1.0037 0.9276 1.0546 1.0263 72 0.6849 1.0584 0.9996 1.0218 100 1.0313 0.9810 0.8905 0.7960 Trannarm: Unlranafomed l-Taila Isotonic Cone-A Meae N-Mean Mean Mm Max CVY.
N t-Slat Crilt1 MOD Mean N-Mean D.Connt 1.0697 1.0000 1.0697 0.9873 1.1611 6.895 4
1.0697 1.0000 10.98 1.0648 0.9954 1.0646 0.9135 1.1573 10.652 4
0.067 2.410 0.1762 1.0648 0.9954 22 0.9917 0.9271 0.9917 0.9267 1.0430 4.866 4
1.067 2.410 0.1762 0.9974 0.9324 43.9 1.0031 0.9377 1.0031 0.9276 1.0546 5.427 4
0.911 Z.410 0.1762 0,9974 0.9324 72 0.9412 0.0799 0.9412 0.6649 1.0584 18.336 4
1.757 2.410 0.1762 0.9412 0.8799 too 0.9247 0.8645 0.9247 0.7960 1.0313 11.215 4
1.993 2.410 0.1762 0.9247 0.8645 aa Fily Th7n Stowe Criial Skew Kurt Sh~zr-W j-
-os T
entindicans a danli p
.01) 0.921115994 0.894
-1.09446415 1.241605879 SBalens Test indicates ~aq vari anucea It, oo 9.06.01902914 15.09631706 iNOEC LOEC CMhv TU MSDu MSDp MSB MSE F-Prob df DAnnews Teas 100
>100 1
0.176189616 0.164717072 0.01459424 0.010689478 0.283319354
- 1.
I8 Linear Interpolato*n (00 Resamples)
Point SD 95%. CL.Exp)
Skew ICOS 1.925 17.057 0.000 107.602 2.59F9 ICI0 61.258
[Cis
>100 1.0 IC20
>10.
IC25
>100 0.9 IC40
>100 0.8 IC50 100 0.7 0.7 0.5 0.4 0.3 0.2 0.1 0.0 0
20 40 60 tS0o 100 120 Dose-Response Plot 1.4 1.2 0.8------
-tail. 0.05 fevel of significance 0.6 0.4 0.2 01 C5. -.
,*,w.
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date:
1/4/02 Test ID:
PpPFCR Sample ID:
TVA SQN - Intake UV Treated End Date:
1/11/02 Lab ID:
ETS-Ezv. Testing Solutions Sample Type:
DMR-Discharge Monitoring Report Sample Date:
Protocol EPAF 91-EPA Freshwatr Test Species:
PP-Pimephaes promelas Comments:
ConC-%
1 2
4 D-Conrol 1.0889 1.0413 0.9873 1.1611 100 1.0089 0.8381 0.9560 0.9800 Transform: Untransformed I-Tailed Conc-%
Mean N-Mean Mean Min Max CV%
N t-Stat Critical MSD lkonnuo 1.0697 1.0000 1.0697 0.9873 1.1611 6.895 4
- 100 0.9458 0.8842 0.9458 0.8381 1.0089 7.925 4
2.356 1.943 0.1022 yAuiliary Tests Statistic Critical Skew Kurt Fpr-Wilks Test indicates normal distibution (p 001) 0.954538047 0.749
-0.44589651
-0.80413952 F-Test indicates equal variances (p = 0.98) 1.032 824516 47.46834564 H ypoth esis T est (1-tail
- 0. 5).
Homoscedastic t Test indicates significant differences Dose-Response Plot 1.4 1.2 1:
,, 0.8 0
S0.6 0.4-0.2.
0 4.
0 it
.i snn OI-n4J-)1.-h
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Methods 1002.0)
Species: Pimephalespromelas Summary of Chemical Analyses TVA Sequoyah Nuclear Plant January 4 - 1I, 2002 221 lilY -Trested Overall Temperature (C) 24.1 25.S 24.6 0.3 24.0 (including all concentrationa for initial and final lenperatures) 25.4 24.6 0
sq lOI-04-0.axk Client.
Tetl dates:
Project number:
Environmental Testing Solutions, 1.l1G Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Pitnephales promelas Daily Chemical Analyses Client:
TVA Sequoyah Nuclear Plant - Nontreated Test dates:
January 4 - 11, 2002 Project number:
221 Concentration Parameter Day 0 Day I Day 2 Day 3 Day 4 Day 5 Day 6 Initial Final Initial Final Initial Final' In tial Final Initial Final Initial Final I11itiay F6 a PH (SU) 8.04 7.82 8.01 7.84 8.2 7.72 8.06 7.78 8.10 7.781--8.081 7.628.9.7 DO (mg/L) 8.1 7.8 8.0 7
7.70 Conductivity (umhos/em) 320 304 3
0 302 314 314 300 Control Alkalinity (mg/i. CaCO3,)
68 65 34 0
Hardness (mg/L CaCO3) 90 90
=, =,,.
m p e r a tu r e ('C )
2 4.6 1 2. 1 2.
4 6 4 92
. 19 S
C 4 62 4 52 4 92 2 4.4 2 4.8 2 5.5 2 4.
2 5.0 2 4.5 1 2 4.7 2 4. 2 S(SUJ) 8.06 779 8.03 7..77 8.04 7.71 8.07 7.75 877
- ..*X.*
In 8.1 7.37.10 76
- 80.
10.98%
P ~ m / )7.9 7.91 8.1 7.51 8.-8.0
.07.
8.0 7.1 8.0 7.28.7.
nductivity (umhos/em) 315 290 298 297 30 tue C) 24.7 24.2 24.5 24.6 25.0 24.3 24.4 24.8 25.4 24.4
- 25.
24.5 24.7 PH (8.07 7.86 8.03 7.76 8.02 7.64 8.06 7.74 8.9 77
.7
.677 D O I
(m g /L )
8. 1
.].
0 8 1
. 78 0
2 2 %
D O ( g i )8 17 98 17 58 17
.1 8.0 7.4 8.1 7.1 7.9 7.1 8.27.
Conductivity (pmNhos/cm) 304 281 288 283 289 295 289 Temperature (,C) 24.7 24.5 24.9 24.3 24.4 24.6 25.4 24.4 25.0 24.5 24.7 pH SU) 8.06 7.9 8.03 7.71 8.06 7.73 43.9%
DO (mg/L) 8.8.3 8.1 7.1 8.1 7.4 8.2 7.2
.0 Conductvity (pmhnos/cm) 27--7 257 261 264 1
Temperature (C) 24.6 24.4 24.9 24.3 24.4 24.7 25.4 24.4 25.1 pH (So) 8.06--
7.94 7ý63 7.97 7.69 8.03
- 71.
87 72%
DO (mg/L) 8.3
-8.5 8
8.3 7.4 8.3 7.01 8.0 Conductivity (pmhou/cm) 242 225 9
226 231 23 Temperature (*C) 24.7 24.1 24.4 24.6 25.0 24.4 24.8 25.4 24.4 24.9 24.7 pH (SU) j 8.05 7.81 7.88 7.67 7.85 7.89 7.67 7.97 7.73 7.99 7.85 DO (mg/i.)
8.5
- 7.
8.5 75 8.2 8.4 8.3 7.1 8.1 8.6 100%
Conductivity (pmhos/cm) 207 192 198 1
Outfall 101 Alkalinity (mg/L CaCO3) 64 61 Hardness (mg/L CaCO3) 78 80 Total Residual Chlorine (mg/L) 0.10
<0.10 Temperature (CC) 24.7 24.1 24.5 24.5 25.0 24.3 24.4 25.4 24.4
.0 24.5 24.7 242 PH (SO 8.03 7.81 7.83 7.71 7.78 7.61 7.83 7.76 7.91 7.77 7.94 7.83 7.76 7.69 DO (mg/L) 8.5 7.9 8.6 7.5 1
7.0 8.4 8.3 7.0 100%
Conductivity (shO-s/cm) 206 192 195 Intake Alkalinity (mg/L CaCO3) 65 62 Hardness (mg/L CaCO3) 80 Total Residual Chlorine (mg/l)
<0.10 Temperature (*C)247 2.1 2.1 26 2.1 2.1 2.1
- 2.
254 244 249 2.1 2.1 mnn fl.fl4fl.*9.F m
Environmenta Testing Solutions, LLC Species: Pimephales promelas Page 6 otr l Client:
O i'LANT Full-strength Chemistry-* OOT7 t.L
/01
/0,
-dAtr&-4 M Parameter pH (S.U.)
DO (mi)
Conductivity
( Imhos/cm)
Alkalinity (mg CaCO/L)
Hardness (InR CaCO 3/L)
Chlorine (m!iL)
Collection start date Collection end time Grab or Composite (duration)
Temperature (QC) upon receipt Physical characteristics Dates sample usedd 0
ETS Project and Sample numbers c
ZiIIIIIiple Nm br,-
I.
I1 8.04 19 z 3Co
'79 7 Lj j
b
-<0.10 0oO2-O a 23-S~aut'
- 0. f Ct
- )-oq-o;*.
,zz 01-6q-0"7. kot-c-oaz Now 01"0(,,-02 0Ia(7-o2 of-al-oz aozoSoJo I I
1234 Co;4f I-t'P CLeAsZ
&CK 01-10-a z.
lo.M4o Date:
()I-a"-O.2
-*. ?',_A naq t,"
e.o 31q go
-145 a
~~~~~~
t..
6
.'2 -,a w-,
I Sample Number:
Fnntrhi Ra*-oh.
I O t-6"*- 6"2 3
8.5 90
"<0. 16 1 "<0-10
Y jurl-umenrtal testing Solutions, LLC Page 6 orf 1 Species: Pimephales promelas Client: T¢VA 4uo,/
MU4 ýu.ý (A-c-Date: 0l-0q-0O--
Full-strength Chemistry: I A)iAKIe Parameter Sam le Number:
1 2
3 pH S (S.U.)
?'. 03 1-1&
"7.q I D6' (m,; )
d Conductivity (Pinhos/cm)
JCq3 Alkalinity (rný, CaCO3/L)
-Z2 Hardness (Mg O
CaCO3/L) go 0
-'4 Chlorine Collection start date Collection end time Grab or Composite 32.IWA4w 25-/0 zs-J40Z*¢Z (duration)
CoMA1/i M
OM*SJ Co f0 rM.
Temperature (0C) upon recei t p__*.
0((-
O.r-C 3-t0 C O
Physical LIr
,geI*-
I characteristics CLAeA CC NO A)O cotLadZ FLORM)Cr.
Co&
rLoAi tg~
M6LACK Dates sample used o1-oq.o2, ot-o(-o2.. O 6 1-j0-0 Z ETS Project and ZZI OZO15.4 Sample numbers OzO.Iq.o07 _.
S. I 3 zo Contrnl Rt-h-
- g. to 314 qo DI 4
I Control Batch*
0 I-C6-07\\
I Control Rateh-61-o¢3.6"
./
Environmental Testing Solutions, LLC U'5 t+/-1. I Species: Pimephales promelas Client:
UO'ifMA cKtOUOe PLA' DailChem Concentration ParC terty
/4 IJ*
Conductivity"
_DO (mg/L)
- 7.
Conductivity
( Dhs/cm) 2 Temperature
(°1(c) 2,q.1 PH (S.U.)
[
oDO(m
,/)
Conductivity (pnos/cm)3I' Temperature (0c)
Ha(S.U.).
ZZ-7.g Conductivity Temperature PH (S.U or,
,,"-DO (Mg/L)
A13.9%
Conductivity Temperature
'=
(0c)
Gq'
/*oo ~DO (rag/L)
- 5 Temperature
/cm)
ZOi7 pHit..
......6 _
Date: ol -
oz-o Dav 1
2
_30S z*j qS 5
Zq.U xqqq 3.
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- 17.
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E~nvironmental Testing Solutions, LLC Page 8ot 10 Species: PNmep halespromela's Clie t:
$ trv4 A M CI~A 2 ~
i,~D ate:
()1-0.4-07.
rn Parameter 3a 4
PH(S.U.)
4
(
0 10 )1. I-7f 1A2
.O Conductivity Concentrami CONTROL l/ooZ~e ITemperature~
DO (rg/L)
Conductivitv (Pnihos/cm)
Temperature (0c)
PH (S.U.)
DOj(mg L)
Conductivitv
(?nnho/cm)
Temperature (OC)
PH (S.U.)
DO LmgL Conductivitv (Inhos/cm)
Temperature (OC) pH (S.U.)
DO (mg/L)
Conductivity (pxnhos/cm)
Temperature
'0C)
PH (S.U.)
D~O (mg)
Conductivity remperature 0C)
- 52..31 314 2424 Zq.1
- 25. I 2-4.
ZS.o) zq.
-Z
- Z.
101,,
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- 1.
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Inia Fia
-niia Fina Initia Fia Iia FIna
Environmental Testing Solutions, L_,GC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Pinephales promelas Daily Chemical Analyses Client:
TVA Sequoyah Nuclear Plant - UV Treated Test dates:
January 4 - II, 2002 Project number:
221 Reviewed by:
Concentration Parameter Day 0 Day I Day 2 Day 3 Day 4 Day 5Dy Initial Fial Initia Fial Initial Fnl Initial Fal Initial Fial]
nta ia nta ia Int Ilial Iita PH (SO 8.001 7.771 8.03 7.74 7.66 7.9
.86 7.3 7.93 7.88 8.04 7.681 7.90 77 DO (mgtL) 8.1 7.81 8.1 7.5 8.0 8.0 7.9 7.3 8.0 7.7 8.0 Conductivity (
os/cm) 3 296 305 301 309 Control Alkalinity (mg"" CaCO3) 65 Hardness (mg/L CaCO3) 90 S,. Temperature CC) 24.6 24.2 24.4 24.6 25.0 24.3 24.4 7
25.4 24.3 24.9 24.7 pH (SIL 8.03 7.68 7.99 7.7 7.81 7.75 7.96 7.0 8.01 7.81 8.03 7.9 7.95 77 10.98%
DO,(mgIL) 8.2 7.8 8.1 8.0 7.2 8.2 8
7.9 7.5 8.2 oqductivity (pehos/acm) 318 296 294 300 296 293 Te tueC)24.6 24.2
- 2.
246 25.01 24.3, 24.4 247 25.4 24.3 24.9 245 24.6
- 2.
PH (I8.06 7.76 7.99 7.811 7.931 7.7 0 8.0 780 8.05 7.78 8.05 76
.4 77 22%
(mgmL) 8.2 7.8 8.3 7.4 8.3
.8 8.0 7.5 Conductfivity (pno/i)305 278 286 82 2892825 Temperature CC) 246 24.2 24.5 24.6 25.0 24.3 24.4
.7 25.4 PH (SU) 8056 7.71 7.99 7.83 7.98 7.71 8.02 77
.6 78
.6 76
.6 77 43.9%
DO(mg/L) 83 7.9 8.4 7.4 8.1 7.2 Conductivity (pmNhos/cm) 277 2
261 257 264 263 264 Temperature CC) 246 24.4 24.6 25.0 24.4 24.7 25.4 24.4 25.1 24.5 24.6 pH (SU) 8.06 7.4 7.99
'7.83 7.98 7.8 8.0 7.7 8.05 7.75 8.06 7.60 7.96 77 72%
DO (mg/L) 8.5 783 7.41 8.1 8.5 8.1 7
1 7.6 8.4 Conductivity (pmhlos/cm) 244 220 228 222 231 222 226 Temperature CC) 24.6 24.2 24.5 24.6 24.9 24.4
- 2.
2 25.0 24.5 24.6 24.2 PH (SU) 8.04 7.71 7.97 7.1 79
.7 7.96 7.72 8.02 7.0 8.07 7.59 7.95 7.77 DO (mg/L) 8.6 7.9 8.3
.2 86 7.7 8.1 7
8.2 76 8.5 7.6 100%
Conductivity (pmhNos/cm) 207 190 194 189
!98 196 Outfal 101 Alkalinity (mg/L CaCO3) 64 61 69 OHardness (mg/L CaCO 3)
Tlotal Residual Chlorine (rag/L)
< 0.10 emper sture C°C) 24-.6 r 42
-452 44 1.1 2.1 2.
50 452 WO
ý(rag/)
8.7-'---
7.18185 77 81 77 837.
100%
Conductivity kos/cm) 207'--90-94 Intake Alkalinity (mg/L CaCO3) 65 Hardness (mg/L CaCO3) 0 T'otal-Residual Chlorine (mg/l.)
<0 STe m pe r a t u r e (
- C )
2 4 6 4 2 4 512
. 12 4
mavironmental Testing Solutions, LLC Page 7 of" jO Species: Pimgephales promelas Client:
eo'*
Kut PAr"Date:
rM
-io-4-2 S~Dav Concentration Parameter.
2 CONTROL PH (SO)
O LV71
_(gmhos/cmn) 23l U{
06 T e m p e r a tu r e (0c) zL. -, (a Z ~~
2q t4 2,1.0 q:
PH (S.U)
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- Conductivity
- -B l
12 (Pmhos/cm) 1 ZS9 2Z1(
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- '7
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-/
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7730(
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7.7 4Z.@%
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=--...*
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Intal Fnal Initial Final Initial If Final
Environmental Testing Solutions, LLC Page 8 or w Species: Pimep hales promelas Client:
PL~c1MI~~~~fA~tz Date:
cc-og-oZ.
Concentration CONTROL
- 01
- 1
) 09 t z.
I oc1 f4 T7ý~
Dav zq.L(1 43{_
-'[
ZqI-21f.-Iq~
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Temperature (0C) pH(SU._)
DO (mg/L)
Conductivity (pinhoS/CM)
Temperature (OC)
P)H (S.U.)
Do (M /L)
Conductivity (Amhos/cm)
Temperature (OC)
PH (S.U.)
DOIT(mg/L)
Conductivity (jimhos/cm)
Temperature (OC)
PH (S.U.)
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Environmental Testin2 Solutions. LLC Alkalinity (EPA Method 310.1)
Matrix Water, MDL = 1.0 mg CaCO3/L Analst 16 Date analyzed
- 0.
1,f.0 Z.
Titrate samples to pH = 4.50 S.U.
Tr n
mu ad
,*denminnaon:
PH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total
= (5 ml NazCO3 x 0.05)1E
= (N x 50000)l 100 ml sample water reference standard ml ml ml
= 0.25/E
= Nx 500
= 4.5 S.U.
number number (E)
(acceptable range - 0.018 - 0.022)
QW-CauVA n -~ 0.0 - 0*
O.1 I
Laboratory control standard..
Reference True value Sample Alkalinity (MV)
% RS = MV/ TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOO/L)
(acceptable range (mg CaCO3/L)
(ml) ml ml ml
=90 to 110%)
l*4%o11i 100 100 12."
2Z.
C.5 LO.(
qe Duplicate s Precision:
Sample Alkalinity.
%RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L)
{(S - D) /t(S+D)/21} x 100 number (ml) ml ml ml (acceptable range =. 10%)
12-22-o1 I
)
4H2.0 too Z-.z EL l
s z
S
-2.-0 I Duplicate D
Matrix spike recovery:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO3/L)
(mg CaCO3/L) ml ml ml mI 16!ýO~o: qO 0
160
-Z,*.
o*
t,0 I.
Sample alkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO3/L)
MV = A - B (acceptable range m CaCO3IL 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity number Samleml ml ml Multiplier (M CaC L)
I Z -14.O
-.rtR H2_0 4o 1.0 q-4q (0 J:)
V'-02-07 j
CI 0
01-04.07 (o0.A
- ..2b.9
(&"A c'3.:.o..
3 L_,6
- 33.
,3 53 01 t).)-
too. 0*4 3.5 o-Reviewed by:
X-48t;
%-, I Pnai.
(
Af 9
Date reviewed: 1 0 \\- ILI-O:Zý
Environmental Testin2 Solutions. LLC Page Z
ofZ Alkalinity S-(EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L~II Date analyzed LI2LL4L-Zq]
Titrate samples to pH = 4.50 S.U.
and multiplier deternination:
pH of Normality (N) of H2SO4 pH Factor or Multiplier Deionized Titrant check Begin Iird-
-=
(5 ml Na2CO3 x 0.05)/E
(N x 50000)1100 mi sample water reference standard ml ml ml 02
NE x500
= 4.5 S.U.
number number (E)
(acceptable range 2*
Laboratorv control standard:
Reference True value Sample Alkalinity (MV)
% RS = MV / TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOIL)
(acceptable range
(-g CaCO-/L)
(ml) ml ml ml
= 90 to 110%)
100 100 O.S
- f. z.-
e 1
QG Cg Duplicate sampe precision:
Sample Alkalinity
%RPD =
Sample Sample ID volume Begin End Total Multiplier (mg ('aCO3/L)
{(S - D) /[(S+D)/21 x 100 number (ml) ml ml ml (acceptable range = = 10%)
Di j1 eesc r?-
IQ'~21-3 110 Duplicate 2o1I2..ol
_o I
too 21 Z. 3 327- 0 O.1
.L D
10 Matrix spike recoverv:
Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (Mg CaCO 3/L)
(ma CaCO3/L)
(ml) ml ml ml l5; o too 13
-510 A 10.t Samplealkalinity (B)
Measured spike value (MV)
% R = MV / SV x 100 (mg CaCO 3/L)
MV = A - B (acceptable range (mg CaCO3tL)
= 75 to 125%)
Sample measurements:
Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO3/L) 0201 lO.OZ r
q.O 0.0-
.0z 100 02oo.ol 2
g2-a3.. w4.
I o
ozo-Z oO I
?--kc- £zo o0 c,'10.o 33.0 30,.
,fa IZ" Envir gon etl
-estin Souios LL5oCi Reviewed by:Daeeewd Date reviewed: I CI - Iq" 0 a7*
Environmental Testin Solutions, LLC P~IGUO A.e Total Hardness (EPA Method 130.2)
Matrix-Water, MDL = 1.0 mg CaCO./L Analyst Date analyzed Sample hardness (B)
Measured spike value (MV)
% R = MV I SV x l00
(-g CaCO3 L)
MV =A -B (acceptable range (mg CaCOfL 75 to 125%)
Sample measurements:
Sample volume Begin End Total Hardness
-m Blank lml nl ml Multi her m CaCQL)
T(should be 0gCaCoL o.-S 2o ororo HS HZ q( I_ -..
I -
I ?
2 -o*.-
a)~ Z
- q oS.0 7-o- ---- _
-Z NoeI~tmolirniused, sample must be diluted.
ReviewedIy Date reviewed [OW'I-OZ0*
Page
Environmental Testin2 Solutinns. TT,C
-ENMOM
-- 6t ra
- .u 01 Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO3/L Date analyzed
_.,a aoycontrol standard.:
RFerne True value Sample Hardness (MV)
% RS =MV /TV x 100 standard number (TV) volume Begin End Total Multiplier (nig caco3IL)
(acceptable range (mngCaCO 3IL)
Ml Im ml ml 90 to 110%)
40 50
- 1.
qSZ.Z HIox
,Matrix spike recovery:
Reference Spike value Sample Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)
(mg CaCO9L) ml) ml ml ml 1I-bCXo 4D0 50 AT-.
Go Sample hardness (B)
Measured spike value (MV)
% R = MVI SV x 100 (rag CaCOIL)
M
= A - B (acceptable range m(mg CaCO3fL)
= 75 to 125%)
a40 eu n Sample measurements:
Sampie number OZoi1Z. 0 1 07-,104.01 D-2o I oq. ol OZOIOS. O0 ob... o0 16. C 2I I
7-021)'%4.
Sample ID (should be = 0 mE CaCO3/L) 3 V--
mUA jjeil mI1 (m) ml ml 50 q1.0 2,o
.1
-71.O Note: If >1 5mj of tifnt= is used, sample must be diluted Reviewed by:
L
=
j Total ml Multiplier 2Z 0
'4. a 3.
Hardness meg CaCO3IL) eo 00 LI Date reviewed [iic ilq'O 7-o-I I
1 Sam 1. -1 d-i Page
.p -i
Environmental Testing Solutions, LLC Page __1ý Page L of__L _
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accunet Model 25 pH/Ion Meter Analyste Date analyzed Calibration:
...*i:/
- ~~:;~~::*./ *
- q::....:.:::::...
0.10 WaIL I
LOft niglT.
Reference standard number Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(ag/L)
(acceptable range = 90 to 110%)
0.50
- 0. t-F9 Duplicate samPle precision:
I Samnle I
Samnl1* ITm Iodide reagent:
Acid reagent:
Slope:
number 0-o.o I.OL
--T-..u ampe caraCtersucs Residual chlorine
%RPD = {(S - D) I[(S+D)/2]} x 100 "iVA (mi1L)
(acceptable range = i 10%)
- 0"{AR M
- L..................
c...............-.S*
I*!.....
...`..*
..*.`....'*:..%.....i.i:...:.*.
Duplicate
- D,<'
I*,O',*,O,,,,___,_,,_______,.,_,
- ,*ii*!** *i
< ocOS*
(7
-
h Reviewed by Date reviewed 01-02-0
Jmnvironmmental i esung niolurons, LLU Page~
Page t of' Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mgfL Meter: Accumet Model 25 pH/Ion Meter Atyanalyt V.
Date analyzed 01" ( C.0 2.
Iodide reagent:
IMRq Acid reagent:
jA 1 t Slope:
Z[ a.
Calibration:
I' ý
- 11 2E 0.10m/L I
1.00 mg/L Reference standard number I
I 1,60 )
Note. For samples with a residual chlorine of > 1.0 mg/L, the calibrafion range must be adjusted to bracket the chlorine levels of the samples.
Laboratory control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV / TV x 100 number (mg/L)
(rag/L)
(acceptable range - 90 to 110%)
I,,J-46W5 0.50 0
I I.
Dula s
le grecsd n:
Sample Sample ID Sample characteristics Residual chlorine
%RPD = {(S - D) /[(S+D)/21} x 100 number
- -N L grklP (a/)(acceptable range =*10%)
4-Duplicate
-SD*,)
Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (ImL)
Blank (shouldbe=<0.10 mL)
ý A--0.
Note: All samples were analyzed in exmess of EPA recomnded holding time (15 mimnue) unless otherwise noted.
Laboratory control standard:
Reference standard True value TV)
Mea(uredevalue (rMe 9RS=MVTVx 10 number (eceL-sta (n1aL)
(Turdptaule rang - 90 to 110%)
S I
-ri.t)0-0.5W" O.!Szz...
gO.t Reviewed by I f*
d Date reviewed
Environmenti Testing Solutions, LLC Page "39 Page of Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter. Accumet Model 25 pH/Ion Meter Analyst t -.
Date analyzed [i-Th.0UI Iodide reagent: [q]oi i I
Acid reagent:
IiK) i
-z 3 Slope: 1 76.0 Calibration:
Reference standard number gr 5
Note: For samples with a residual chlorine of > 1.0 mg/L, the calibration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorv control standard:
Reference standard True value (TV)
Measured value (MV)
% RS = MV I TV x 100 number (mg/L)
(mg/L)
(acceptable range = 90 to 110%)
It4 *&O~S 0.50 o
ic
% RS= MV/TVx IO0
-ceptable range - 90 to 110%)
Reviewed by Date reviewed [::0 1 -1 7-,1e
Sequoyah Nuclear Plant Biomonitoring January 4 - 11, 2002 Appendix D Reference Toxicant Tests and Control Chart Information I-
Environmental Testing Solutions, LLC tassium Chloride Chronic Reference Toxicant Control Cha for Pimeph ales promelas using Moderately Hard Synthetic Water Test date 7-day IC25 (g KCI/L) 03-07-00 03-17-00 05-23-00 06-13-00 06-13-00 09-19-00 10-24-00 11-07-00 03-13-01 06-26-01 07-1 7-01 08-21-01 09-25-01 11-01-01 11-06-01 11-27-01 12-12-)1 0 1-04-02 0.5845 0.6755 0.6873 0.7239 0.7014 0.6570 0.7678 0.6523 0.6874 0.5334 0.7438 0.6179 0.6008 0.7105 0.6573 0.5741 0.6324 0.4878 CT (g/L KC1) 0.6300 0.6491 0.6678 0.6745 0.6716 0.6853 0.6812 0.6819 0.6671 0.6740 0.6694 0.6641 0.6674 0.6667 0.6609 0.6593 0.6497 S
0.0643 0.0563 0.0592 0.0535 0.0483 0.0572 0.0542 0.0508 0.0670 0.0677 0.0665 0.0665 0.0651 0.0627 0.0649 0.0632 0.0734 CV CT-2S CT+2S Test number 1
2 3
4 6
7 8
9 10 II 12 13 14 15 16 17 18 0.5013 0.5366 0.5493 0.5676 0.5749 0.5710 0.5728 0.5804 0.5330 0.5387 0.5363 0.5311 0.5373 0.5412 0.5312 0.5328 0.5029 0.7587 0.7616 0.7863 0.7814 0.7683 0.7997 0.7896 0.7834 0.8011 0.8094 0.8024 0.7970 0.7975 0.7922 0.7907 0.7857 0.7966 0.1287 0.1125 0.1185 0.1069 0.0967 0.1144 0.1084 0.1015 0.1341
- 0. 1354
- 0. 1331 0.1329 0.1301 0.1255 0.1298 0.1264 0.1469 Note:
7-d IC25 = 7-day 25%. inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in PI l epha/e..
CT=
S=
5.\\.75=
growth tor the test population.
Central tendency (mean IC25).
Standard deviation of the IC25 values.
-th Standard deviation corresponding to the the 75 percentile CV.
S..75 = 0.38. as determined by USEPA for the method and endpoint.
Sz.qo = Standard deviation corresponding to the the 901hpercentile CV, SA.9o = 0.45, as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values.
SEPA. 2000. Understanding aind Accounting for Method Variability in Whole Effluent Toxicity pplications Under the National Pollutant Discharge Elimination Progurm. EPA-833-R-00-003. US wironmental Protection Agency, Cincinnati, OH.
Onganisms obtained fr'om Aquatic BioSvstems, Inc.
SQN Reftox 01 02-CLR.xts 2SD 10.21%
8.67%
8.87%
7.92%
7.20%
8.34%
7.96%
7.44%
10.05%
10.04%
9.94%
10.01 I%
9.75%
9.41%
9.82%
9.59%
11.30%
Environmental Testing Solutions, LLC
Cd for Pirnephales prortelas using Moderately Hard Synthetic Water COD tA to 0)%
roo Control Jim its Set According to + 2 Standard Deviations.
I.0 0
0 d
b6 U
0.8 0"1~~...........
° 0.6 L
(mean....5.....Standar.............
a-° 0.4 0tQ i,/
Cetral Tndency mean t25))
0 7....
dayoro 1C25t 25%inibtinI2 conceStraniond Dialinestiaino)hcnetaino W
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimeph ales promelas using Moderately Hard Synthetic Water est number Test date 7-day IC25 CT S
SA.7 SA.9 0 CV CT - St, CT - S, CT-T CT-CT-S CT-Z (g KCI/L)
(gIL KCI) 1 03-07-00 0.58 2
03-17-00 0.68 0.63 0.06 0.24 0.28 0.10 0.25 1.01 0.18 1.08 0.50 0.76 4
3 05-23-00 0.69 0.65 0.06 0.25 0.29 0.09 0.27 1.03 0.20 1.10 0.54 0.76 4
06-13-00 0.72 0.67 0.06 0.25 0.30 0.09 0.29 1.05 0.22 1.12 0-55 0.79 5
06-13-00 0.70 0.67 0.05 0.26 0.30 0.08 0.29 1.05 0.22 1.12 0.57 0.78 6
09-19-00 0.66 0.67 0.05 0.26 0.30 0.07 0.29 1.05 0.22 1.12 0.57 0.77 7
10-24-00 0.77 0.69 0.06 0.26 0.31 0.08 0.31 1.07 0.24
.14 0.57 0.80 8
11-07-00 0.65 0.68 (1.05 0.26 0.31 0.08 0.30 1.06 0.23
.13 0.57 079 9
03-13-01 0.69 0.68 0.05 0.26 0.31 0.07 0.30 1.06 0.23 1.13 0.58 0.78 10 06-26-01 0.53 0.67,
.07 0.25 0.30 0.10 0.29 1.05 0.22 1.12 0.53 0.80 II 07-17-01 0.74 0.67 0.07 0.26 0.30 0.10 0.29 1.05 0.22 1.12 0.54 0.81 12 08-21-01 0.62 0.67 0.07 0.25 0.30 0.10 0.29 1.05 0.22 1.12 0.54 0.80 13 09-25-01 0.60 0.66 0.07 0.25 0.30 0.10 0.28 1.04 0.21 1.))
0.53 0.80 14 11-01-01 0.71 0.67 0.07 0.25 0.30 0.10 0.29 1.05 0.22 1.12 0.54 0.80 15 11-06-01 0.66 0.67 0.06 0.25 0.30 0.09 0.29 1.05 0.22 1.12 0.54 0.79 16 11-27-0 1 0.57 0.66 0.06 0.25 0.30 0.10 0.28 1.04 0.21 1.11 0.53 0.79 17 12-12-01 0.63 0.66 0.06 0.25 0.30 0.10 0.28 1.04 0.21 1.11 0.53 0.79 18 01-04-02 0.49 0.65 0.07 0.25 0.29 0.11 0.27 1.03 0.20 1.10 0.50 0.80
,kte:
7-d 1(C25 7-dav 25"0 inhibition concentration. Anl estimation of the concentration o1 polttiSstiun chloride that would cause a 25% reduction in Pinepholes "growth tor the test population.
CT - Central tendency (mean 1C25)
S = Standard deviation of the IC25 values.
SA."-
Standard deviation corresponding to the the 7 5 h percentile CV.
S,.75 = 0.38. as determined by USEPA forthe method and endpoint.
SA.10 =Standard deviation corresponding to the the 9 0"' percentile CV.
SA.W = 0.45. as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values.
EPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National lutant Discharge Elimination Prognam. EPA-8-33-R-00-O3. US Eniaronmental Protection Agency. Cincinnati. Ott.
Organisms obtained fiom Aquatic BioSv'srens. Ic.
01-04-02.xls
.'U
,., ?
'S U
0."
1.0 0.5 Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pinephales prom elas using Moderately Hard Synthetic Water Warning and Control Limits Set According to 75' and 9e Percentile CVs.
Since (he coefficient ofvariation for the set of 7-d IC25 values (011) is less than the 75'h and 90'h percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
I I
II I
I I
I I
I I
I I
I Test date 7-day IC25 =25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
Central Tendency (mean IC25)
Warning Limhis (mean IC25 +/- S A*5, SA. 5 = 0.38, as determined by USEPA for the method and endpoint)
- ......Control Limits (mean 1C25 +/- S..90 SA.9 = 0.45, as determined by USEPA for the method and endpoint) o-.
(-4 2.0 1.5 CO CD.
en I-.
oh 0.0 L-i
'-1 H
0 r
'-3 H
U) t'.
6)¢.
14
-i
°..............
JAN.13.2002 12:21 8288828195 Environmientaf Testing Snlnitinne TI" ENVIRONMENTAL TESTING SOLUTIONS
- 1127 P.024
-IPage oi Potssium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephales promnelas PpKCICR Test Number.
Daily feeding and renewal information:
JAN-13-2002 11:32 8288628195 P. 024
JANJ1.3.2002 12:21 8288628195 EnlvironmrentWi Testing Soowiu,*ya T T d-ENVIRONMENTAL TESTING SOLUTIONS
- 1127 P.025 P~age 2 of 5 PpKCICR Test Number: j A Plk.j vLraeih (mg Ii~Fz~
+ Laa
.weight (nmg)~.
'ry~o mg)
Wv A0
'31 eX<
Calculations 11(i dýta rmiewed:
T'2
ýC L
8288628195 JAN-13-2302 11:32 P. 025
-JAN.13.2002 12:2! 8298828195 ENVIRONMENTAL TESTING SOLUTIONS
- 1127 P.026 Environmental Testing Sofuflons LLC Page 3 01 FPpKCICR Test Nuunbir:
J Li mY z
CLL (04 514 10
/0 /0
'v )
14 4 7I 5 M9jI KCY 900ma II/
=1
'u%
ILI p Q
I TIV I W I x q10
-7
.q
/6 73
(.0 lo C)
LI0 1144 1Id Z4 4'
10 IL t]
4 1d a
I I
I J
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i l d
rv a an rowt], Data Day 600 SO z
1*
T
9h
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k
-.
I'.v..:,
,I
ii
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A.
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-~
.~47 ILk 4r,~-
0 3
4 6
7 A P m weighit(1110 B=mP21 Larvahtjgt()
JAN-13-2J02 11:132 8288628195 P. 026 C-c',nents.:
i AD$!
1 -
Environmental Testing Solutions, LLC Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival art Date:
1/4/02 Test ID:
PpKCICR Sample ID:
f(EF-RefToxicant Ad Date:
1/11/02 Lab ID:
ETS-Env. Testing Solutions Sample Type:
KCL-Potassium chloride mple Date:
Protocol:
EPAF 91-EPA Freshwater Test. Species:
PP-Pimephales promelas
ýmments:
anc-nmg/L 1
2 3
4 D-Control 1.0000 0.9000 0.9000 1.0000 300 0.7000 0.8000 1.0000 1.0000 450 0.8000 1.0000 0,7000 0.9000 600 0.5000 0.7000 0.6000 0.6000 750 0.3000 0.9000 0.0000 0.2000 900 0.0000 0.0000 0.!1000 0.1000 Transform: Arcsin Square Root I-Tailed Number Total Donc-mgt/L Mean N-Mean Mean Min Max CV%
0/
N t-Stat Critical MSD Resp Number D-Control 0.9500 1.0000 1.3305 1.2490 1.4120 7.072 4
2 40 300 0.8750 0.9211 1.2306 0.9912 1.4120 17.454 4
0.617 2.410 0.3907 5
40 450 0,8500 0.8947
- 1. 1898 0.9912 1.4120 15.281 4
0.868 2410 0.3907 6
40
- 600 0.6000 0.6316 0.8872 0.7854 0.9912 9.469 4
2.735 2.410 0.3907 16 40
- 750 0.3500 0.3684 0.612S 0.1588 1.2490 75.038 4
4.427 2.410 0.3907 26 40
- 900 0.0500 0.0526 0.2403 0.1588 0.3218 39.161 4
6.725 2.410 0.3907 38 40
.xiliary Tests Statistic Critical Skew Kurt apiro-Wilk's Test indicates normal distribution (p 0.01) 0.91273692 0.88-i 0.908581907 3.811713373 rtlett's Test indicates equal variances (p = 0.0.3) 12.67539597 15.0,63177,6 "pothesis Test (l-tail, 0.11)
NOEC LOEC ClhV TUT MSDu NISDp NISB MSE F-Prth (If nnett's Test 450 600 519.6152423 0.291389424 0.3088797N8 0.716140()72
- ).05_2564,29 1.4E-05 5, 16 Maximum Likelihood-Probit ralneter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter
,pe 10.12820705 1.891366178 6.42112927 13.83528483 0.05 2.342762563 7.814724922 0.5 2.825479568 0.098734158 7
5.3945961
-14.19045065 -13.04363353 0.030778369 0.033697731l 0.154148938 ng/L 950/6 Fiducial Limits 394.2662185 274.4358598 '468.8424496 460.3374728 349.3406584 526.7559271 499.9731179 396.9361633 561.0171676 528.6256032 432.3835662 585.7579873 552.5642792 462.5425809 606.5348106 573.9636523 489.8144023 625.2941241 611.6340829 563.7161328 677.7697729 669.0823441 610.5906447 714.7586265 708.7508417 657.3746155 758.3392309 779.964338 730.2949578 851.539691 810.170332 757.2813023 896.5085251 846.8587038 787.8892431 954.4793911 895.3904742 825.8761911 1035.63639 972.4847434 882.5994399 1172.726823 1135.454154 994.5752609 1488.379701 1.0 0.9 0.8 0.7 0..6 C0.5 Q0.4 0.3 0.2 0.1 0.0 10 100 Dose mg/L Organisms obtainedfrom Aquatic BioSystems, Inc.
01-04-02.xls
-rcept CR ilt 01 05 10 15 20 25 40 50 50 75
)0 85
/0
-)5
)9
-23.61704209 0.094023334 Probits 2.674 3.355 3.718 3.964 4.15N 4.326 4.747 5.000 5.253 5.674 5.842 6.036 6.282 6.645 7.326 1000 10000 l
(I 0.9 0.8 0.7 1 -tail, 0.05 level of significance 0.6 c 0.5 I.
0.4 0.3 0.2 0.1 0 o CD 0
0C0 0
LO 0
)O 0 C-,
Environmental Testing Solutions, LLC S"
Statistical Analyses art Date:
1/4102 id Date:
1/11/02 ample Date:
)mments:
Test 1):
P 1pKC3CR Larval Fish Growth and Survival Test-7 Day Growth Sample ID:
REF-Ref Toxicant Lab MD:
ETS-Env. Testing Solutions Protocol:
EPAF 91-EPA Freshwater Sample Type:
Test Species:
KCL-Potassium chloride PP-Pimephales promelas tin-ng/
12 3
4 0-Control 0.S12S 0.6814 0.8459 0.8569
'00 0.5199 0.54K9 0.7242 0.7697 450 0.72-72 0.7815 0.4815 0.6551 600 0.2539 0.5903 0.4658 0.4759 750 0.1028 0.4731 0.0000 0.1340 900 0.0000 0U000 0.0564 0.0555 Transform: Untransformed I-Tailed
'nnc-nmg/L Mean N-Mean
- Mean Mill Max CV%
N t-Stat Critical MSD
.0D-Control 0.7993 1.0000 0.7993 0.6b:14 0.8569 10.106 4
'00 0.6407 0.8016 0.6407 0.5199 0.7697 19.460 1.964 2.180 0.1 450 0.6613 0.8274 0.6613 0.4615 0.7815 19.746 4
1.708 2.180 0.1 600 0.4465 0.5586 0.4465 0.2539 0.5903 31.413 4
750 0.1775 0.2221 0.1775 0.0000 0.4731 115.639 4
900 0.0280 0.0350 0.0280 0.0000 0.0564 115.478 4
isotonic Mean N-Nlean 0.7993 1.0000 760 0.6510 0.8145 760 0.6510 0.8145 0.4465 0.5586 0.1775 0.2221 0.0280 0.0350 xiliarv Tests Statistic Critical Skew Kurt piro-Wilk's Test indicates normal distnbution (p ý 0.01) 0.91874N081 0.805
-0.488905I02 -l.l38415106 pi~thicsis fest (I-tail. 1.1**5)
NOEC LOEC nnett's Test 450
- 450 0.64969360, 9.21035099 ChV TU MSDu MSDp
- INlS1, NISE F-Prmib df 0.176029231 3.^220243017 0.029770506 0.013040269 (0.158107191 2.9 Lhinear Interpolatioin (80 Resaniples) nt 95/I.
,I) 95% CL(Exp)
Skew
- .87 1.08 29I9 N
18.6s 2.1810 061.74 118.92 5N.39 647.06 1.0958 5*
242.61 122.21 87.58 637.39 0.3472
.0 458.51 113.20 35.76 569.35
-0.3797 5
4N7.82 92.49 95.89 607.45
-1.2155
.0 575.74 37.16 48K.77 681.85
.0.0123
- 0 626.12 40.22 525.00 778.65 0.8685 dicates IC estimate less than the lowest concentration 0
200 400 600 800 1000 Dose mg/L Orgcainsms oblahied from Aquatic BioSystems. Inc.
1.0 0.9 0.8 0.7 w 0.6 C
C-.
COL0.
5 im 0.4 0.3 0.2 0.1 0.0 01-04-02.xls Linear Interpolatiun (80 Resamplesl
V&
0.9 0.8 0.7 1-ta il, 0.0 5 leve l 0.6 of significance "Q0.5 0.4 0.3 0.2 0.1 0
D0 0
0 0
0 0
Environmental Testing Solutions, LLC Chronic Whole Elfluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Pimeplhtles promelas Qua)I lit y (Colntrol Verif'ication 44f Data Entry, ('11h6Ihi~lioS, anIld Stafisfica! An~alyses T est nunmber:
Pp)KCIl(R 4 1I)
Test daltes:
January 4-I11, 2001 RIe'eiwed hy:
Conten ha'ion(mg./L I Replicate I inifialng i eof I Fit h
f..
,.I A....
=
.P.,.
I t
(m4 B..=.
t I aa
-... I..
I
, eight (n1g) l,al I
tic g~l~llt l[
.f 1ar M Iglgg)
= A - R of h;IN ae (mag) 1)eall suliVal M%
ileail weight (ing)
Coefficient of vahifalicn (%)
Percent reduction from, control (%)
A 10 10 14.972 23.100 8.128 0.8128 Control C
10 9
14.941 23.400 8.459 0.8459 95.0 0.7993 10.1 Not applicable r
10 9
14.996 21.810 6.814 0.6814 D
10 10 1-1.851 23.420 8.569 0.8569 E
10 7
15.041 20.240 5.199 0.5199 300 F
10 8
15.011 20.500 5.489 0.5489 87.5 0.6407 1965 19.8 G
10 to 14.828 22.070 7.242 0.724 8
II t0 10 14.983 22.680 7.697 0.7697 "1
10 8
14.858 22.130 7.272 0.7272
.1 10 10 14.825 22.6.10 7.815 0,7815 K
10 7
15.055
,9.870 4.815 0.4815
- 1.
10 9
14.979 21.530 6.551 0.6551 NI 10 5
14.931 17,470 2.539 0.2539 600 N
10 7
14.877 20.780 5.903 0.5903 0
1 10 6
15.052 19.710 4.658 0.4658 60.0 0.4465 31.4 44.1
'_ 1 10 6
14.901 19.660 4.759 0.4759 Q
10 3
14.832 15.860 1.028 0.1028 750 R
10 9
14.869 19.600 4.731 0.4731 S10 01 0.000 0.000) 01.000 0.0000 3.11.751567.
10 2
1.1.870 16.210 1.340 0.1340 U
10 0
0.000 0.000 0.000 0.0000 900 V
10 0
0.000 0.000 0.000 0.0000 W
10 I
14.996 15.560 0.564 0.0564 X
10 I
14.985 15.540 0.555 0.0555 Dunnett's NISD value:
PAISD:
0.176 22.0 PT/S l) ý-
M inim um Signifi eam t liffe rence 2 0IM S I)
IP e rc e n t
,M
.iiinm u m S ig n i fic a n t D iffe ren c e PIvSD is a measure of test piecision. I he PNMSD is the min imtun percent difference between the control and treatment that can be decla red statistically signilicant in a %%hotle elfluent toxicity test. On average, a signi camt) diflerence occurs lor Environmental Testing Solutionis 1.1 C ci
'oilic toxicity tests %%
hen a toxicalnt i
'educes l'im eilhales grow th 1y 9
- 19.
Im fiom the conlurol (detcn ined thriough LIowcr l'PNSI) boutnd de lcmniiled by USIOIA (101h pcicciitle)
O 4%.
Ilipper P1NSI) bound deternined by USEPA (90111 p ecentile) -. 35%.
T h e loam eC a n d m mp p 'e i b o umn d s at C w e c a lc u hla e d l b y th e I JS I "IA u si ni g 2 0 5 te sts c o ni d u c te d fi o m 1 9 la b o r a t oii e s fo r l' im epl h a le s g row th it) chronic IcClClcice IoxiCanlt tests.
USEPA. 2000 Understanding and Accounting fbr Method Va jabiliy in Whole 1:h111tet Toxicity Applications Under the Natotonal P*ollutam ischarg e
I llion Piogram E PA-833-R-00-003 US Environmental Protection Agency, Cincinnati, OI1.
Orgemtismms oblcined from t
Aquatic BioStwem.u. hi'.
01-04-02.xls KCI) la-iae tla-vae I..
January 4-1 I, 2001
,6,,, I,,,bj
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimeph ales promelas using Moderately Hard Synthetic Water Test number Test date Control Survival
(%)
1 2
3 4
5 6
7 8
9 I (I 10 12 13 14 15 16 17 18 0)3-07-00 (13-17-00 05-23-00 (06-13-00 06-13-00 09-19-00 1 0-24-O0 11-07-0(0 03-13-01 06-26-0 1 (7-17-01 (08-21-01 09-25-01 1 I-(0I-01 1l1-06-01 11-27-01 12-12-01 (01-04-02 1(00 97.5 100 100 100 100 97.5 97.5 92.5 100 1OO 100 100 1O0 10(1 97.5 95 95 Control Mean Growth (mg, arn'ae) 0.67
(.40 0.31
(.45 0.58 0.67 0.83
(.67 0.34 0.63
(.52
(.89 0.85
(.54 1.00 0.70 1.82
(.80 CV CT for Control Growth CV (%)
4.9 7.5 3.6 10.1 13.2 5.3 13.3 10.5 6.5 9.8 9.1 8.3 4.9 2.5 7.4 22.8 16.6
- 10. 1 6.2 5.3 6.5 7.9 7.4 8.3 8.6 8.3 8.5 8.5 8.5 8.2 7.8 7.8 8.7 9.2 9.2 MSD P
(.05
(.06 0.07
- 0. 10
(.12 0.07
(.22 0.13 0.08 0.()79 0.13 0.09 0). 10 (0.17
- 0. 18 0.19 (1.18 hMSD CT (O0) for PMSD(%)
7.6 15.7 21.2 22.2 20.0(
11.2 26.9 19.3 22.8 3o.6 13.8 15.1 10.4 18.6 16.9 26.1 23.5 22.0 11.7 14.8 16.7 17.3 16.3 17.8 18.0 18.5 19.7 19.2 18.9 18.2 18.2 18.2 18.7 18.9 19.1 V - Coefficient of variation tor control survival.
On average. the CV ror control growth is 9.2% in Environmental Testing Solutions. LLC Pimoiep/h.eschronic toxicity tests.
L.ower CV bound determined by USEPA (I0't percentile) 3.51, Upper CV bound determined by UJSEPA (90"' percentile) 200,,
SSD I)
Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average. a sienificant difference occurs for Environmental Testing Solutions. LLC chronic toxicity tests when a toxicant reduces Pinteohales zrowth bv 19.1% from the control.
Lower PMSD bound determined by USEPA (10"' percentile) =9.40.
Upper PMSD bound determined by IJSEPA (90"' percentile) = 350a.
CT - Central Tendancv (mean Control Growth CV or mean PMSD)
The lower and tipper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories tbr Pintephales growth in chronic reference toxicant tests.
USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environniental Protection Agency. Cincinnati, OH.
Oiganisms obrained fi'on Aquatic BioSysteni.s, Inc.
01- 0 4-02.xls
-4
Environmental Testing Solutions, LLC Precision of Endpoint Measurements for PiN epizales promelas t
using Moderately Hard Synthetic Water CD UN 01)N ODc CD' I
40 300 rn
'-I L-a 20 N
0 I-A 01 20 II4 IOI I90 I
al Test date F_
--- PMSID percent minimnum significant difkbrenc¢. I'M SD is the minimuni significant difference
,OA S~between the control and treatment, that can be declared sta~tistically significant.
O
¢9
-- -Central "'rendenry (mean PMISD)
LI........
L~ower and Uppe'r PMISD Bounds Lower PMSD Bound (1le percentile) = 9.4%, Upper PM-SD Bound (90'b percentil6) = 35%
(Lower and upper PMSD bounds were determnined by USEPA for the method and m~dpoint.)
V.
JAN.13.2002 1.2:21. 8288628195 Environmnental Testinc nii;'.
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