Text

December 2001 Discharge Monitoring Report for Sequoyah Nuclear Plant
	ML020430548
Person / Time
Site:	Sequoyah
Issue date:	01/09/2002
From:	Nida D; Tennessee Valley Authority
To:	Haskin E; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	Download: ML020430548 (94)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37379-2000 January 9, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control 401 Church Street, 6th Floor Annex Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin, Enforcement & Compliance

Dear Ms. Haskin:

TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT (SQN) Please find enclosed the Discharge Monitoring Report (DMR) of December 2001 for SQN. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority j for Richard T. Purcell Vice President Sequoyah Nuclear Plant

Enclosures:

2 (original & copy) cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 4

Na-me _ TVA - SEQUOYAH NUCLEAR PLANT AosLress _.BOX 2_- (INTEROF IC_aE SB- -2.A'I j _.U Y_ - TM 73N - - - - - -N I o(.tionhHAMiQJO.N_LVO DISCHARGE MONITORING REPONI (LJA'wr1 (2-16) (17-19) TN02450 O101 pERMITI NUMBER DISCHARGE NUMBER l ~MONITORING* PERInn IMQ A ,yYFAr I M I DAY_ From l01 11210I To 01 12 31 'P.- O' 'P 1 1 )P /)A-')7% M-901 (.1n-31) I (SUBR 01) F -FINAL DIFFUSER DISCHARGE EFFLUENT OMB No. 2U04-UU04 Approval expires 05-31-98 NO DISCHARGE [] NOTE: Read instructions before completing this lorm. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM DATETELEPHONE FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY TELEPHONE7/~,4 Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE 'g, INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE I__ANME 18 U.S.C. §1001 AND33 U.S.C. §1319. (Penalties underthesestatutes mayinclude finesup to OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED 1$10,000 and or maximum imprisonment ol belween 6 months and 5 years.) COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Refereqce oil attachments here) No closed mode operation. The following Injections occured: 1. PCL-222 (max. calc. conc. was 0.025mg/L--limit 0.100mg/L) 2. CL-363 (max. calc. conc. was 0.008mg/L--limit 0.100mg/L) 3. PCL 222/PCL-401 (max. cabc. cono. was 0.02mg/L--limit 0.100mg/L) S., -,,r,," n,,u,,J"L kAAv M,",T gi, , c n P~nnn 1 at 2 EPA Form 3320-1 (08-95) Previous editions may not be used. Pane I of 2 (REPfLACES: EPA/ rurt IV V I -4

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N__me TVA - SEQUOYAH NUCLEAR PLANT Ad.e.ss BO 2.X 2 -00 __ -- JNT5g.Om S D __-2A__MR SODD*-fAJ$_ _ TiN 373------ Fciilv.V1 A -S,*UEUQYAH.UL.AI?.UL.NL--- I-oDleodreHA.QNld ATTN: Diedre B. NIdo PARAMETER DISCHARGE MONITORING REPORT L 4Mi (SUBR 01) (2-16) (17-19) TN0026450 101 F - FINAL PERMIT NUMBER DISCHARGE NUM DIFFUSER DISCHARGE I MONITO[I?1NG PE'c5)ia-= EFFLUENT OMB No. 2040-0004 Approval expires 05-31-98 From I I I ToI AP L -t... NO DISCHARGE F "1 1.1) 12~ 01 To 01 (12 31 NOTE: Read instructions before completing this form. "(3 Cord Only QUANTITY OR LOADING 4 Card Only QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE (46-53) 354-61) (38-45) (4653 (54-6d Only EX OF TYPE MAXIMM 1UITS[ VERAEITS (62_63) A64A8LY (9I70 AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE - C, RATE OF SAMPLE

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CALCTD EFFLUENT GROSS VALUE TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2.7 (04) 0 31/ 31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 0 0 T DEG. C. CAWTD VEURE MENTý EFFLUENT GROSS VALUE '~~M¶ SAMPLE MEASUREMENT WAR: SAMPLE MEASUREMENT ,CPERMIT "RýQUIF(EMENT MEASUREMENT BORON, TOTAL SAMPLE <0.2 <0.2 <0.2 (19) 0 1 /31 GRAB MEASUREMENT 01022 1 0 0 REPORT .REPORT REPORT MGJL GRAB EFFLUENT GROSS VALUE NJ SAMPLE MEASUREMENT .FEQUIItMEMNT. NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM TELEPHONE DATE NAMETITL PRICIPA EXEUTIV OFFCER FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY '/ -"/ Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE 18 U.S.C. §1001 AND 33 U.S.C. §1319. (Penalties under these statutes mayinclude lines up to OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED $10,000 and or maximum imprisonment o1 between 6 months and 5 years.) COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenqe oal attachments here) CCW data for December 2001 is attached. EPA Form 3320-1 (08-95) Previous editions may not be used. (REPLACES EPA FORM T-40 WHICH MAY NOT BE USED) Page 2 of 2 PARAMETER

CCW Data for December 2001 CCW CHANNEL DATE DIESEL RANGE TOTAL PETROLEUM COMMENTS ORGANICS HYDROCARBONS (mgIL) (mgIL) 12/05/01 < 0.1 <1 12/19/01 0.6 <1 Backup samples sent to the lab for DRO. CCW TRENCH DISCHARGE DATE DIESEL RANGE TOTAL PETROLEUM COMMENTS ORGANICS HYDROCARBONS (mg/L) (mg/L) 12/05/01 0.67 <1 12/19/01 0.51 <1 Backup samples sent to the lab for DRO. Page 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Noame TVA - SEQUOYAH NUCLEAR PLANT Ad!.e ss BOX 2000 5__.*._DDy.:_.QýBy__ _ MN334_ Fo.cility TV._A -F_.UYH_ULARPI I ncation. HAM------N---------- ATTN: Diedre B. Nida NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) q (SUBR 01) (2-16) ( 117-19) I TN0026450 I101 F - FINAL PERMIT NUMBER IDISCHARGE NUMER DIFFUSER DISCHARGE I MC)NITO2lN* PFR I.) 4 ] EFFLUENT YA MC) I I FA IMNO DI Froml 01 1 I 01 To 01 NO12 3 SCHARGE L Read instructions before cot Form Approved. I OMB No. 2040-0004 Approval expires 05-31-98 mpleting this form. ('M-91) (J.A ) (.'6-97N (9n-" Q (3f,,31N.4 PARAMETER (3 Card Only QUANTITY OR LOADING 4 Card Only QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE L46-53) -653) (5-1)-k (38-4.... EX OF TYPE (32-37) AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS > <(62-63) (64-68) (69-70) IC25 STATRE 7DAY CHR SAMPLE >100 (23) 0 1 / 92 GRAB CERIODAPHNIA MEASUREMENT TR3B 1 0 ~ .PEMMlT~.~~*: PERCENT QUART-GRAB EFFLUENT GROSS VALUE Ž,. } 'yMINIMUM, ERLY IC25 STATRE 7DAY CHR SAMPLE

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PIMEPHALES MEASUREMENT (23) 4' TRP6C 1 0 0 PER4IT. K' 439 PERCENT QUART-GRAB REOUIRIEME.NT .MIIU RY EFFLUENT GROSS VALUE -:M SAMPLE MEASUREMENT SAPL MEASUREMENT SAMPLE MEASUREMENT ýPERMIT I REQUIRIEMENT SAMPLE MEASUREMENT PgRMIT'l -t SAMPLE MEASUREMENT SAMPLE MEASUREMENT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM 7 TELEPHONE DATE FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE z 13C INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE 18 U.S.C. §1001 AND 33 U.S.C. §1319. (Penalties under these statutes may include lines up to OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED $10,000 and or maximum imprisonment of between 6 months and 5 years.) OFFICERO_ AUTHORIZDAGENTAEA NUMBER YEARMO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (ReferenCe all attachments here) Toxicity was sampled from 11/25 to 11/30. The test was terminated due to a Fed-Ex delivery error. Toxicity was sampled 12/9 to 12/14. The test results were determined to be invalid. A repeat test was initiated from 1/2 to 1/7. The follow-up test results will be reported within 30 days of test invalidation, according to the permit requirements. A detailed explanation and the report are attached. EPA Form 3320-1 (08-95) Previous editions may not be used. (REPLACES EPA FORM T-40 WHICH MAY NOT BE USED) Page 1 of 1

PERMITTEE NAME/ADDRESS (include Faciltiy Name/Location if Different) Nnme PVA - SEQUOYAH NUCLEAR PLANT A..d...SS. __OX 21X00 __ _ (INTEROFFICE S-2A) FQ_.PA-__Y__ - M U 3 FAqiitrN: DiVr B. Nlda HIQLfAULANL----- LomtinH-AN WIJQNQLNIY-------- ATTN: Diedre B. Nida PARAMETER (32-37) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) t (2-16) (17-19) TN06450 10 --PErRMIT NUMBER DISCHARGE NUME L~yMQNIT(OIN-- PERIOD YFAR MQ I QA* F.yAQ I MQ DAY From1 01 To2 1 1 To 31 tot-in /0 0 t NIS (3 Cord Only QUANTITY OR LOADING (46-53) (54-61) AVERAGE MAXIMUM UNITS (211--2-a c9 oru kuiey k-UI-i k A~l --.. S F MINIMUM (46-53) AVERAGE MAJOR Form Approved. (SUBR 01) OMB No. 2040-0004 F - FINAL Approval expires 05-31-98 LOW VOL. WASTE TREATMENT POND EFFLUENT NO DISCHARGE !I] NOTE: Read instructions before completing this form. ICENTRATION NO. FREQUENCY SAMPLE 15*-Al.l.... EX OF TYPE7 MAXIMUM UNITS ANALYSIS (62-63) (64-68) (69-70) PH SAMPLE 7.0 8.7 (12) 0 13 /31 GRAB PH ,,MEASUREMENT 00400 1 0 0 1P=BMIT=..,., 9,0 SU THR I GRAB EFFLUENT GROSS VALUE -,.,,"MINIMUM.,. MAXIMUM'___ WEEK 01 0 4 / 3 1 G R A B SOLIDS, TOTAL SUSPENDED SAMPLE 98 132 (26) (19) MEASUREMENT 9 ,.,-.;:;;*)*:c* l}:'*'*c LBSD .**-*0 0 -,,. - :.! 0 MGdL !W EEKLY'"GRAB 00530 1 0 0 80 2 BSIDY 1W EFFLUENT GROSS VALUE .IBE, I....4AVGI5 AILY MXDI__ -L 0 A J MX M O A G<ALY M 0 4 /31 GRAB OIL AND GREASE SAMPLE <50 <52 (26) (19) MEASUREMENT LBS/DY 'MG/L GRAB 00556 1 0 0 9 0 -5 ..x, 20 EFFLUENT GROSS VALUE bDAILV' X { ! iAll.,:MK_ FLOW, IN CONDUIT OR THRU SAMPLE 1.163 1.246 (03) 31 / 31 TOTAL TREATMENT PLANT MEASUREMENT 50050 1 0 0 itEf~TV ,.PRý1ý EFLETGROSS VALUE M;,.*'.** NT.:*-";*°* r>,' .;,MeAVG ,<REDAIY M.T; MGD

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REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM TELEPHONE DATE FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE / INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATEAND Environmental Supervisor 423 843-6700 02 01 10 COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE 18 U.S.C. §1001 AND 33 U.S.C. §1319. (Penalties under these statutes may.include lines up to OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED $10,000 and or maximum imprisonment of belween 6 months and 5 years.) CR' "R)F COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here) EPA Form 3320-1 (08-95) Previous editions may not be used. I Cord OnOUlly /'IQ A -*", AVERAGE .U. ý (REPWLACES-E-PA FORM 1 -40 VV Hun VI i NO I'* i D UOE

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Adgr..ss __OX 2C INTECROFFICE SB-2A) Fcilitv

VA -- F---U-----A--P Location H A AM IQb1 JNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR DISCHARGE MONITORING REPORT (DMR) 4 (2-16) (17-19) (SUBR 01) TN0026450 r17 G F-FINAL PRINUBR DSCHARGE NUMR METAL CLEANING WASTE POND tA (-AnlIITnDINirt PCFPDItf I EFFLUENT IFo AP MO I DAY I YEAR MO IDAY From I01 12 1 01I To 1 01 1 31 ATTN: Dledre B. Nida Form Approved. OMB No. 2040-0004 Approval expires 05-31-968 NO DISCHARGE XX NOTE: Read instructions before completing this form. ____________(21121) (99-91) f94-9 (26-27Y) (2-9 3113i tIr)___ PARAMETER (3 Cord Only QUANTITY OR LOADING 4 Card Only QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE (46-53) (54-61) (38 (46-531 .(541AL&D - EX OF TYPE (32-37) AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS > <(62-63) (64-68) (69-70) PH SAMPLE (12) MEASUREMENT (12) 00400 1 0 0 .0 SU DAILY GRAB EFFLUENT GROSS VALUE -' MINIM UM-MAXIMUM 4 PHOSPHORUS, TOTAL (AS P) SAMPLE (19) MEASUREMENT 00665 1 0 0 r.Y RI.T 1.... 7 1.0 MG/L DAILY COMP-8 EFFLUENT GROSS VALUE Q.- ,r- <1/2IA vw-'DAILY MX SOLIDS, TOTAL SUSPENDED SAMPLE 1** (9) MEASUREMENT 00530 1 0 0 77# N-.- MGLDIY OM EFFLUENT GROSS VALUE DAILY.- MX OIL AND GREASE SAMPLE (19) MEASUREMENT 00556 1 0 0 ..R...T 15 MGIL DAILY GRAB EFFLUENT GROSS VALUE COPPER, TOTAL (AS CU) SAMPLE MEASUREMENT (19) 01042 1 0 0 7-i. 10 MG/L DAILY COMP-8 REQUIREMEN~T 4 ~ EFFLUENT GROSS VALUE DA IL Y DAYMX IRON, TOTAL (AS FE) SAMPLE 9**) MEASUREMENT (19) 01045 1 0 0 i1.0.IT.V 1.0 MG/L DAILY COMP-8 RQUI~REýENT,

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~ EPORT MGD DIY CLT EFFLUENT GROSS VALUE RýPVIFIT,-.. MbAVG,< :DAIFLY MX NT' IX NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED-AND AM TELEPHONE DATE F-AMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE TE INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 t10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE 18 U.S.C. §1001 AND 33 U.S.C. §1319. (Penalties under these statutes may include fines up to OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED $10,000 and or maximum imprisonment of between 6 months and 5 years.) OREA COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a/l ottachments here) No phosphate bearing chemicals were employed. No Discharge this Period (REPLACES EPA FORM T-40 WHICH MAY NOT BE USED) Page 1 of 1 EPA Form 3320-1 (08-95) Previous editions may not be used.

PERMITTEE NAME/ADDRESS (Include Facility" Name/Location if Different) Name TVA - SEQUOYAH NUCLEAR PLANT A__d a _ox2000 INTEiOfEIC_ B--2A t QD -_DISy - T a 4 ------- t~ocation .H AMATUL.IC FcIlv _V -sDUYAHjU~LLC1R2L NL---- ~t-ocjitn-HI~lQNCQUN1y-------- ATrN: Dledre B, NIda NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) (2-16) (17-19) TN402 W 10oG PERMIT NFr DISCHARGE NUMBER YFAR MO lDAY I I..yA!RI Mn IDA From olI 012 lITlOl 1-12! 1 MAJOR (SUBR 01) F - FINAL RECYCLED COOLING WATER EFFLUENT Form Approved. OMB No. 2040-0004 Approval expires 05-31-98 SNO DISCHARGE XX NOTE: Read instructions before completing this form. PARAMETER (3 Cold Orny QUANTITY OR LOADING 4 Cord Only QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE (46-53) (54-61) (38-451.... EX OF TYPE (32-37) AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS

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(64-68) (69-70) PH SAMPLE 2**) MEASUREMENT 00400 1 0 0 .6.0........,.0 DAILY GRAB EFFLUENT GROSS VALUE MINIMUM MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE 9 MEASUREMENT (1 00530 1 0 0 0'PER!IIT 3 MG/L DAILY GRAB EFFLUENT GROSS VALUE UF..., T DAILY NIX OIL AND GREASE SAMPLE (19) MEASUREMENT 00556 1 0 0 ...I.... MG/L DAILY P GRA S...................._______ ______________MG _______/L___ EFFLUENT GROSS VALUE DAILY MX COPPER, TOTAL (AS CU) SAMPLE MEASUREMENT****(19) MEASUREMENT 01042 1 0 0 ' EBiI*0K;. -.v,'* *# 1.0 MG.L DAILY GRAB EFFLUENT GROSS VALUE j,, 1.-, 1' DAILY MX I IRON, TOTAL (AS FE) SAMPLE (19) MEASUREMENT 01045 1 0 0 .2!PER IT 1.0 MG: L DAILY GRAB EFFLUENT GROSS VALUE -DAILY NIX PHOSPHORUS, TOTAL (AS P) SAMPLE (19) MEASUREMENT 00665 1 0 0 \\PIERMiT*r-MG/L DAILY GRAB EFFLUENT GROSS VALUE DAILY MX....._.. FLOW, IN CONDUIT OR THRU SAMPLE (03) TREATMENT PLANT MEASUREMENT 50050 1 0 0 MG DAIL GRA EFFLUENT GROSS VALUE M,- ENT , :GD. DAIY GR. 'M AVG' L.. DAILYMKNX-NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM //. TELEPHONE DATE FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE I 18 U.S.C. §1001 AND 33 U.S.C. §1319. (Penalties under these stalutes mayinclude lines up to OFFICER OR AUTHORIZED AGENT ER YEAR MO DAY TYPED OR PRINTED I$10,000 and or maximum imprisonment of between 6 months and 5 years.) F COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Re feence all attochments here) No Discharge this Period r'age Itof I (REPLACES EPA FORM T-40 WHICH MAY NOT BE IUSE-D) EPA -Form 3320-1 (08-95) Prvoseiins may not be used.

NPme PTVA - SEQUOYAH NUCLEAR PLANT N----- QD&.DY&DSY _ N37384_ FociltL_ A -_ *.CL.*RPIA t ocAtion.l-eeW.O NdO-a ATTN: Dledre B. Nida DISCGHARLiE MONI IUHINU* htt-Iur 1-1-Vs (2-16) (17-19) 65 0 Ii PERMIT NUMBER DISCHARGE N1MI From 01 112101 I To 1 01 12 31] (SUBR 01) UMB No. ZU4U-UUU4, r F - FINAL Approval expires 05-31-98 WASTEWATER & STORM WATER EFFLUENT

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fl f ATh3-I Md) IOIý 2 (3 Cord OnJy QUANTITY OR LOADING( (460-53) 1101-12 1LS~ _______ M9 11___ -I AVERAGE MAXIMUM UNITS ('.z*A.A1 EX (62-63) OF ANALYSIS (64-68) SAMPLE TYPE (69-70) OXYGEN, DISSOLVED (DO) SAMPLE (19) MEASUREMENT 00300 1 0 0 2.0 EFFLUENT GROSS VALUE i,*,;,,jgX :DAILY. MN'..*_ WE SOLIDS, TOTAL SUSPENDED SAMPLE 109* MEASUREMENT 00530 1 0 0 ~ PRl

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,i': MG/ TW,,.E/ RA EFFLUENT GROSS VALUE REP_________ DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE (25) MEASUREMENT 00545 1 0 0 IT R.1.Q.o MLJL ONCE/ GRAB S::i !;i::;i~;! AIL M XMONTH E F F L U E N T G R O S S V A L U E R. ýQ U R..; - - ,,M NH.. FLOW, IN CONDUIT OR THRU SAMPLE (03) TREATMENT PLANT MEASUREMENT 50050 1 0 0 .R:*RlT. REPQRT REPOTý,. MGD ONCE/ CALCTD EFFLUENT GROSS VALUE T .MO A DAILY MX. BATCH SAMPLE MEASUREMENT REQ*JIREMENT SAMPLE MEASUREMENT PEERMIT 'FlQJIJERVMENT SAMPLE MEASUREMENT .REQUIjEMgNT NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I CERTIFY UNDER PENALTY OF LAW THAT I HAVE PERSONALLY EXAMINED AND AM TELEPHONE DATE FAMILIAR WITH THE INFORMATION SUBMITTED HEREIN; AND BASED ON MY INQUIRY L. ( Richard T. Purcell OF THOSE INDIVIDUALS IMMEDIATELY RESPONSIBLE FOR OBTAINING THE INFORMATION, I BELIEVE THE SUBMITTED INFORMATION IS TRUE, ACCURATE AND Environmental Supervisor COMPLETE. I AM AWARE THAT THERE ARE SIGNIFICANT PENALTIES FOR SUBMITTING 423 843-6700 02 01 10 Site Vice President FALSE INFORMATION, INCLUDING THE POSSIBILITY OF FINE AND IMPRISONMENT. SEE SIGNATURE OF PRINCIPAL EXECUTIVE 18U.S.C.§1001 AND33U.S.C.§1319. (Penalties under these statutes may include lines up to TYPED OR PRINTED $10,000 and or maximum Imprisonment of between 6 months and 5 years.) OFE RAT IZAN(_:_")__FAREANUMBERYEARMODAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oal attachments here) During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period -,-,,-nrr~nn~rAn In, -IV' klAInfT Of= "Oroa InI I k A(%I*IITFI*INI(*_ 131::131(%1"3 Co4 ( Of nly WoUMLlll UK %,UI*IVýL14K^III1 tAA &q\\ u"I MINIMUM AVERAGE MP,*IMUM (REPI[*~:bIIAr~ llI-u v lnIl N I-U I-J EPA Form 3320-1 (08-95) Previous editions may not be used.

January 8, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIONIONTTORiNG, NPDES PERM[IT NO. TN0026450, NOVEMBER AND DECEMBER 2001 Attached are two copies of the subject report for submission to the state of Tennessee with the DMR package. The report provides results of compliance testing using daphnids only due to invalidation of the concurrent fathead minnow test. I have also included a copy of the report for your records. SQN Outfall 101 samples collected December 9-14 were not toxic to daphnids (IC25 value > 43.9 percent). Although the fathead minnow test "passed" using either point estimate or hypothesis test statistical analyses (i.e., IC25 = 75 percent and NOEC = 100 percent), the test had low statistical sensitivity due to the anomalous dose response and highly variable survival among replicates within treatments. which appeared to be related to the intake water (intake survival = 40 percent). Exposure of fathead minnows to intake samples resulted in statistically significant reductions for both survival and growth. No intake toxicity to daphnids was demonstrated. Permit language allowing data review according to current EPA procedures showed that the point estimate (1C25) was the correct test endpoint for compliance determination, but that the percent minimum sianificant difference for the test exceeded the EPA recommended range for test sensitivity. Using this rationale, the test was ruled invalid and a repeat test was initiated January 4, 2002. Since results from the December test showed symptoms typical of pathogen interference associated with the intake water, the follow-up test also includes a parallel test using samples treated by exposure to UV light to eliminate pathogen interference, if present. Bench sheets from the invalid test are presented in Appendix E. The follow-up tests will be reported within 30 days of test invalidation, according to the permit requirements. Test invalidation and follow-up test schedule were agrees upoRn in email and phone messages between Jennifer Moses, TVA, and Chris Moran, TDEC, on December 20 and 21, 2001. The first attempt to conduct SQN fourth quarter biomonitoring was initiated on November 27, 2001. These tests were terminated on December I (Day 4) due to failure by FedEx to deliver weekend samples. Bench sheets for these tests are presented in Appendix F. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CEB 3A-M Attachment cc (Attachment): Files, ER&TA, CEB 1B-M 4

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION/EXECUTIVE

SUMMARY

1) Facility/Discharger: Sequovah Nuclear Plant/TVA Report Date: January 8. 2002

2)

County/State: Hamilton/Tennessee

3) NPDES Permit #: TN0026450

4)

Type of Facility: Nuclear-fueled electric generating plant

5)

Design Flow (MGD): 3,266

6)

Receiving Stream: Tennessee River (TRM 483.6)

7) 1Q20: 2.992.4

8)

Outfall Tested: 101 .9) Dates Sampled: December 9-14. 2001

10) Flow on day(s) sampled (MGD): 1600, 1605. 1599. 1598. 1605. 1589

11) Pertinent site conditions: No unusual conditions reported.*

12) Test Dates: December 10-17. 2001

13) Test Type: Short-term Chronic-definitive

14) Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15) Concentrations Tested(%): Outfall 101 10.98 22 43.9 72 100 Intake 100

16) Permit Limit Endpoint (%): Outfall 101 ICj - 43.9

17) Test Results (%): Outfall 101 1 Pimephales promelas ICQ5 = Test Invalid (See Appendix E)

Ceriodaphnia dubia IC_ 5 >1 00

18) Facility

Contact:

Diedre B. Nida

19) Phone #: (423) 843-6700

20) Consultant/Testing Lab Name: S&ME Inc.

21) Lab

Contact:

Leira S. Douthat Phone #: (865)-970-0003

22) TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

Production/operation data will be provided in the event toxicity exceeds permit limits/upon request.

23) Notes: SQN Outfall 101 samples collected December 9-14 were not toxic to daphnids (IC 25 value>

43.9 percent). Although the fathead minnow test "passed" using either point estimate or hypothesis test statistical analyses (i.e., IC2 5 = 75 percent and NOEC = 100 percent), the test had low statistical sensitivity due to the anomalous dose response and highly variable survival among replicates within treatments, which appeared to be related to the intake water (intake survival = 40 percent). Exposure of fathead minnows to intake samples resulted in statistically significant reductions for both survival and growth. No intake toxicity to daphnids was demonstrated. Permit language allowing data review according to current EPA procedures showed that the point estimate (IC25) was the correct test endpoint for compliance determination, but that the percent minimum significant difference for the test exceeded the EPA recommended range for test sensitivity. Using this rationale, -the test was ruled invalid and a repeat test was initiated January 4, 2002. Since results from the December test showed symptoms typical of pathogen interference associated with the intake water, the follow-up test also includes a parallel test using samples treated by exposure to UV light to eliminate pathogen interference, if present. Bench sheets from the invalid test are presented in Appendix E. The follow-up tests will be reported within 30 days of test invalidation, according to the permit requirements. Test invalidation and follow-up test schedule were agrees upon in email and phone messages between Jennifer Moses, TVA, and Chris Moran, TDEC, on December 20 and 21, 200 1. The first attempt to conduct SQN fourth quarter biomonitoring was initiated on November 27, 2001. These tests were terminated on December 1 (Day 4) due to failure by FedEx to deliver weekend samples. Bench sheets for these tests are presented in Appendix F. 2

METHODS

SUMMARY

Samples

1) Sampling Point: Outfall 101 and Intake

2) Sample Type: Composite

3) Sample Information:

4) Sample manipulation: Daily samples warmed to 25+/-10 C Test Organisms

1) Source:

2) Age:

Pimephales promelas In-house Culture < 24 hours Ceriodaphnia dubia In-house Culture < 24 hours 3 Date/Time Date/Time Arrival Initial Date/Time ID Collected Received Temp. TRC* Used By (MM-DD/Time) (MM-DD/Time) (°C) (mrnL) (MM-DD/Time) 101 12/09-0922 to 12/10/1232 0.3 0.07 12-10/1430 12/10-0822 12-11/1500 Intake 12/09-0940 to 12/10/1232 0.5 0.15 12-10/1430 12/10-0840 12-11/1500 101 12/11-0909 to 12/12/1210 0.9 0.08 12-12/1445 12/12-0809 12-13/1415 Intake 12/11-0926 to 12/12/1210 1.4 0.06 12-12/1445 12/12-0826 12-13/1445 101 12/13-0826 to 12/14/1235 2.0 0.07 12-14/1330 12/14-0726 12-15/1330 12-16/ 1345 Intake 12/13-0841 to 12/14/1235 2.0 0.05 12-14/1330 12/14-0741 12-15/1330 12-16/1345

Total residual chlorine upon receipt.

Test Method Summary (See Appendix A for additional information)

1) 2)

Test Conditions Test Duration

3) Dilution/Control Water

4) Number Replicates

5) Animals per Replicate

6) Test Initiation

7) Test Termination

8) Test Temperature Pimephales promelas N/A N/A N/A N/A N/A N/A (Date/Time)

N/A (Date/Time) Mean = N/A Ceriodaphnia dubia Static.- renewal Until at least 60% of control females have 3 broods 20% Dilute minerals water and trace minerals 10 I 12-10-01/1430 (Date/Time) 12-17-01/1500 (Date/Time) Mean = 24.7°C (24.0°C-25.5 0C)

9) Physical/Chemical Measurements: Hardness, alkalinity, total residual chlorine, and conductivity were measured at the laboratory in each 100 percent sample. Daily temperatures were measured in one replication per test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10) Statistics: Toxcalc software Version 5.0 was used for statistical analyses. Test data were reviewed for dose response, sensitivity, and validity following recent EPA guidance. [2] [3]

4 4

2) Results of a Ceriodaphnia dubia (Type/Duration)

Conducted 12/10/01 12/17/01 (mm/dd/yy) (mm/dd/yy) Using Effluent From Outfall 101. (number) Percent SuNiving Test Solutions (time intervals, used - days) 1 2 3 4 5 6 7 Control 100 too 100 100 100 100 100 10.98%Effluent 100 100 100 100 100 100 100 22.0% Effluent 100 100 100 100 100 100 100 43.9% Effluent 100 100 100 100 100 100 100 72.0% Effluent 100 100 100 100 100 100 100 100% Effluent 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Reproduction (# of young/female during test) Data Test Solutions Replicates 1 2 3 4 5 6 7 8 9 10 Mean Control 28 31 22 26 24 26 24 24 28 24 25.7 10.98%Effluent 26 25 26 22 20 24 28 2 24 24 24.1 22.0% Effluent 30 24 30 23 28 28 28 23 29 25 26.8 43.9% Effluent 31 29 25 24 29 28 25 24 24 24 26.3 72.0% Effluent 24 27 29 27 22 23 24 23 23 23 24.5 100% Effluent 27 27 30 23 28 24 25 21 26 22 25.3 Intake 30 32 23 25 26 27 26 22 ? 24 25 26.0 IC, 5 Value: > 100% Calculated TU Estimates T: <1.0 TUc 95% Confidence Limits Permit Limit: 2.3 TUc Upper Limit NA Lower Limit NA NOTE: TUa = 100/LCio; TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS Species Date Time I Ceriodaphnia dubia 12/10/10 1530 Results (LCs0/IC2 5)

1. 117 mg/L NaCI 5

(Genus) (Species) Chronic/ 7 day Q3-brood) Toxicity Test

PHYSICAL/CHEMICAL

SUMMARY

1) Analysis of Sequoyah Nuclear Plant Diffuser Discharge Concentrations of Total Residual Chlorine

2) Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling

3) Initial and Final Chemistry for Fathead Minnow 7-day and-Daphnid 3-brood Chronic Tests.

See Summary Tables in Appendix B

SUMMARY

/CONCLUSIONS SQN Outfall 101 samples collected December 9-14 were not toxic to daphnids (IC2 5 value > 43.9 percent). Although the fathead minnow test "passed" using either point estimate or hypothesis test statistical analyses (i.e., IC25 = 75 percent and NOEC = 100 percent), the test had low statistical sensitivity due to the anomalous dose response and highly variable survival among replicates within treatments, which appeared to be related to the intake water (intake survival = 40 percent). Exposure of fathead minnows to intake samples resulted in statistically significant reductions for both survival and growth. No intake toxicity to daphnids was demonstrated. Permit language allowing data review according to current EPA procedures showed that the point estimate (IC25) was the correct test endpoint for compliance determination, but that the percent minimum significant difference for the test exceeded the EPA recommended range for test sensitiYity. Using this rationale, the test was ruled invalid and a repeat test was initiated January 4, 2002. Since results from the December test showed symptoms typical of pathogen interference associated with the intake water, the follow-up test also includes a parallel test using samples treated by exposure to UV light to eliminate pathogen interference, if present. Bench sheets from the invalid test are presented in Appendix E. The follow-up tests will be reported within 30 days of test invalidation, according to the permit requirements. Test invalidation and follow-up test schedule were agrees upon in email and phone messages between Jennifer Moses, TVA. and Chris Moran. TDEC, on December 20 and 21, 2001. 6

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1) Pimephales promelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL polystyrene cups, each containing 250 mL of test solution. [4]

2) Ceriodaphnia dubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polystyrene cups, each containing 15 mL of test solution. [4]

DEVIATIONS/MODIFICATIONS TO TEST PROTOCOL

1) Pimephales promelIas None

2) Ceriodaphnia dub ia None DEVIATIONS/MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS I) Pimephales pronmelas None

2) Ceriodaphnia dubica None 7

4

PHYSICAL AND CHEMICAL METHODS

1) Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2) Instruments: All identification, service, and calibration information pertaining to S&ME laboratory instruments is recorded in calibration and maintenance log books.

3) Temperature was measured using a VWR Scientific digital NIST-traceable thermometer according to S&ME SOP. [5]

4) Dissolved oxygen was measured using a YSI Dissolved Oxygen meter. The instrument was calibrated and readings were made according to EPA Method 120.1.

5) The pH was measured using a VWR Scientific Symphony pH meter equipped with an Orion combination electrode. The instrument was calibrated and readings were made according to EPA Method 150.1.

6) Conductance was measured using an Orion conductivity meter. The instrument was calibrated and readings were taken according to manufacturers instructions.

7) Alkalinity was measured using Standard Methods Titration Method 23-20 B using 0.01 N HCl to an endpoint pH of 4.5.

8) Hardness was measured using Standard Methods EDTA Titrimetric Method 2340 C or EPA Method 130.2.

9) Total residual chlorine was determined using the DPD Ferrous Titrimetric Method with a Hach colorimetric kit.

QUALITY ASSURANCE ' Toxicity Test Methods: All phases of the study including, but not limited to. sample collection, handling and storage; glassware preparation; test organism culturing/acquisition and acclimation; test organism handling during test; and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. [4] Any known deviations were noted during the study and are reported herein. 8

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1) Test Type: 7-day chronic tests with results expressed as IC25 values in g/L NaC1 or g/L KC1.

2) Standard Toxicant: Sodium Chloride and Potassium Chloride (NaCl and KCI crystalline)

3) Dilution Water Used: Moderately hard water for fathead minnows and 20 percent DMW water for the Ceriodaphnia dubia.

4) Statistics: Toxcalc software Version 5.0 was used for statistical analyses.

REFERENCES

1) NPDES Permit No. TN0026450.

2) Methods Guidance and Recommendationsfjbr Whole EJIhuent Toxicity (WET) Testing (40 CFR Part 130). EPA 821-B-00-004 (July 2000).

3) Understanding and Accountingfor Method V-ariabilit, in Whole Effluent Toxicity Applications Under the National Pollutant Discharge System, EPA 833-R-00-003 (June 2000)

4) Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber. Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).

5) S&ME Standard Operating Procedures. April. 2000.

9

Sequoyah Nuclear Plant Biomonitoring December 10-17, 2001 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams. During Toxicity Test Sampling, And Initial and Final Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests II

Table B-1. Analysis of Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Total Residual Chlorine*, December 10 - December 14, 2001

Towerbrom-960 (Oxidizing Biocide) 7% Sodium Bromide, 89% Sodium Dichloroisocyanurate is utilized in the essential raw cooling water and in the raw cooling water systems at SQN to control the growth of microbiologically induced bacteria and Asiatic Clams. The treatment is necessary for adequate cooling water flow, efficient heat exchange and corrosion prevention. The injected chemical is diluted by the condenser cooling water flow as well as from the flow from other sources.

Table B-2. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-December 14, 2001 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-130M hypochlorite PF mg/L TRC mgIL TRC mg/L mg/L mgiL mg/L Azole mg/L - Phosphate Copolymer DMAD Quat 03/12/1998 0.016 03/13/1998 0.015 03/14/1998 0.013 03/15/1998 0.030 03/16/1998 0.013 03/17/1998 0.020 03/18/1998 0.018 09/08/1998 0.015 0.014 0.005 0.021 09/09/1998 0.003 0.031 0.011 09/10/1998 0.014 0.060 0.021 09/11/1998 0.013 0.055 0.019 09/12/1998 < 0.001 0.044 0.015 09/13/1998 < 0.001 0.044 0.015 09/14/1998 0.008 0.044 0.015 02/22/1999 < 0.001 02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999 < 0.001 02/28/1999 < 0.001 08/18/1999 0.015 0.069 0.024 0.006 08/19/1999 0.012 0.068 0.024 08/20/1999 0.023 0.070 0.024 0.120 08/21/1999 0.022 0.068 0.024 08/22/1999 0.022 0.068 0.024 08/23/1999 0.025 0.068 0.024 0.0016 08/24/1999 0.016 0.067 0.023 0.020-

Table B-2 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-December 14, 2001 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-H-_'30M hypochlorite PF mg/L TRC mg/L TRC mg/L mg/L mg/L mg/L Azole mgIL Phosphate Copolymer DMAD Quat 01/31/2000 0.002 0.026 0.009 02/01/2000 0.011 0.026 0.028 02/02/2000 0.028 0.026 0.009 0.006 02/03/2000 0.008 0.027 0.009 02/04/2000 0.006 0.027 0.009 0.005 0.109 02/05/2000 < 0.002 0.027 0.009 02/06/2000 < 0.002 0.027 0.009 07/26/2000 < 0.0057 0.055 0.019 0.021 07/27/2000 0.019 0.055 0.019 07/28/2000 0.0088 0.053 0.018 0.004 0.108 07/29/2000 0.0088 0.055 0.019 07/30/2000 0.0076 0.055 0.019 07/31/2000 <0.0152 0.055 0.019 0.006 08/01/2000 0.0141 0.055 0.019 0.005 12/11/2000 0.0143 0.025 0.020 0.005 12/12/2000 0.0092 0.025 0.020 0.005 12/13/2000 0.0120 0.025 0.020 12/14/2000 0.0087 0.025 0.020 12/15/2000 0.0120 0.025 0.020 0.005 12/16/2000 0.0036 0.025 0.020 12/17/2000 0.0036 0.025 0.020 08/26/2001 0.017 0.06 0.021 0.006 08/27/2001 <0.0096 0.06 0.021 0.005 0.021 08/28/2001 <0.0085 0.06 0.021 08/29/2001 <0.0094 0.059 0.020 0.005 0.021 08/30/2001 <0.0123 0.06 0.021 0.005 08/31/2001 <0.005 0.059 0.020 11/25/2001 <0.0044 11/26/2001 <0.0119 0.024 0.02 0.005 11/27/2001 0.0137 0.023 0.019 0.007 11/28/2001 <0.0089 0.022 0.019 0.006 11/29/2001 0.0132 0.024 0.02 0.007 11/30/2001 < 0.0043 0.024 0.02 12/09/2001 <0.0042 12/10/2001 <0.0042 12/11/2001 <0.0104 12/12/2001 0.0128 0.024 0.02 0.008 12/13/2001 <0.0088 0.024 0.02 12/14/2001 0.0134 0.024 0.02 0.007 I I [ __I_ 4

Table B-3. Water Chemistry Mean Values and Ranges for Daphnid Test, Sequoyah Nuclear Plant (SQN), December 10 - 17, 2001 Temperature Dissolved Oxygen pH1 Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Initial Initial Chlorine Sample ID ('C) (oC) (rag/L) (mag/1') S. U. S.J. (p.tlnhos) ,ng/L CaCO3 mg/L CaCO3 (mg/L) Daphnid/ 24.7 24.9 7.3 7.0 8.0 -8.0 164 63.0 91.7 Control (24.0-25.2) (24.3-25.5) (7.1-7.6) (6.3-7.8) (7.9-8.2) (7.9-8.1) (134-181 ) (60-67) (90-95) Daphnid/ 24.7 25.0 7.4 7.0 8.1 8.1 170 10.98 (24.0-25.4) (24.4-25.4) (7.1-7.8) (6.3-7.7) (8.0-8.1) (8.0-8.2) (152-183) Daphnid/ 24.7 24.9 7.3 7.1 8.1 8.1 173 22.0% (24.0-25.2) (24.4-25.4) (7.1-7.6) (6.6-7.8) (8.0-8.1) (8.0-8.1) (159-185) Daphnid/ 24.7 24.7 7.3 7.1 8.0 8.1 179 44.0% (24.0-25.3) (24.0-25.3) (7.1-7.5) (6.7-7.7) (7.9-8.0) (8.1-8.2) (168-188) Daphnid/ 24.7 24.5 7.2 7.2 7.9 8.2 186 72.0% (24.0-25.3) (24.0-25.1) (7.0-7.5) (6.9-7.7) (7.8-7.9) (8.1-8.3) (178-194) Daphnid/ 24.8 24.5 7.3 7.2 7.8 8.2 194 66.3 76.7 0.07 100.0% (24.0-25.4) (24.0-25.0) (7.0-7.8) (6.9-7.8) (7.6-7.9) (8.1-8.3) (183-205) (63-69) (70-80) (0.05-0.08) Daphnid/ 24.8 24.6 7.3 7.2 7.7 8.0 195 68.3 76.0 0.09 Intake (24.0-25.3) (24.1-25.2) (7.0-8.0) (6.7-7.8) (7.4-7.8) (7.3-8.2) (190-205) (67-70) (72-80) (0.05-0.15)

Sequoyah Nuclear Plant Biomonitoring December 10-17, 2001 Appendix C Chain of Custody Records and Toxicity Bench Sheets

Cli.nt: Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number: PO BOX 2000

acility Sampled: SEQUOYAH NUCLEAR PLANT IPDES Number: TN0026450

'oilected By: WANDAALLEN Field Identlfication / Sample Description Collection Date/Time. (if grab, list date/time under "End") 1413 Topside Road, Louisville, TN 37777 Phone: 865-970-0003 Fax: 865-970-0004 Container Number & Volume Collected Flow (MGD Other (specify): ructions: Clents should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 400 and shipping them l,** for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ampleqifiA ing vithin that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Mond.a E-d Ups Bus . Ee-i-S & ME, Inc. -i-S.* A....

Client: IVA Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number: PO BOX 2000 =acility Sampled: SEQUOYAH NUCLEAR PLANT VPDES Number: TNo026450 ýollected By: WAN DAALLEN Field Identification I Sample Description 1413 Topside Road, Louisville, TN 37777 Phone: Fax: Collection Date/Time (If grab, list date/time under "End") 865-970-0003 865-970-0004 Container Number & Volume Flow (MGD Other (specify):. tructions: Clents should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 4"C and shipping them i;n o.ThIjI e for each sample Is 36 hours from the time of collection. Therefore, please collect andship in such a way that the laboratory will receive the samples with ampletimo,IT ling within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monda'

,q.*oc v*.*,,*

J i I EeJ=.aE. Ups Bus S & ME, Inc.

Client: IVA Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number -P0 BOX 2oo0 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: TNO02645o Collected By: WANDA ALLEN Field Identification / Sample Description Collection Date/Time (If grab, list date/time under "End") S & ME, Inc. 1413 Topside Road, Louisville, TN 37777 Phone: 865-970-0003 Fax: 865-970-0004 Container Number & Volume Flow (MGD Other (specify): strctWions:., Clents should fill In all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 40C and shipping them Wi'd lie for. each sample is 36 hours from the time of collection, Therefore, please collect and ship in such a way that the laboratory will receive the samples with ampleilm stiOg Within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Mor"da Fe~ UPS Bus

T Test Name: TVA -Sequovah Nuclear Plant Test Conducted From: m k Sites/Concentrations:

1.

4,

2. io~q%

5.

3. ý;

Z::,

6.

Stock (if applicable): Control Water Type (1: 20% Dilute Mineral Water + Trace Minerals Other (describe): Dilution Water Type (v): 20% Dilute Mineral Water + Trace Minerals Other (describe): Source of Test Organisms: S&ME brood board (Day 0) To

3-i22
3,~od n

E nos. k(, Age of Test Organisms: Isolated neonates for test on -- o (date) from to \\40 ( (time) Initials LS. 0 Record of Minor Test Non-Conformity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed k (Reviewer Initials) on (Date) REV.0 1, January 00 (ceriodaphnia wt inf sheet) (Day 7)

tno*.

\\*

Ceriodaphnia Daily Test Information Logsheet TestName: TVA - Sequoyah Nuclear Plant Test Dates: 12/10/01 - 12/17/01 Daily Test Temperature Information Test Initiation, Water information (cup -25+/-+1 C) Feeding Information Change, or Test Termination Control Water Carboy No. and Date and Incubator Temp. (0C) Therm. Fed 1i0' uL Fed 100 uL Selanastrum StartAdditional comments Initials No. YCT YCT DatSelenastrum Date Day0-a.m. p.m. .Carboy Date Prep. Day 0 i_'lat V 1 so C:) Day3

Day4 2 \\...

I1IILIIrt 2-.. I -7jL712l 14LO iia\\t} j:c O I IIn131 0 l Day 5 Day 6 VCS Day 7 ./,l/, 2-..

- - - 12-___-

'7-Client: Tennh&Vallzjahonftv.-euoy yss MO ý Location: Soddy baisvr Tennessee-TstSatDeTiei 5 ho l4i Dates Samples Collected: 1.l. zrz.~j Test Stop.-DatetTime: 14111toi 1.s?4) I I Conc. Contro DMW 20% _________~~ I I

~ U'-

-5'. I I 'L 112131415 6 7 8 9 110 Number of Vn~lQ A 711 ~ A,.1,, AA..IL 1C 2 f_) -ycs 3 n L J~t4 6 -M 4 ri S-I 5 4 I.~ &,ý_ 5-1 -7 15 _CIC 0,4

of Live Numof of Orignal Young per I

I. &Z %-/ I-L~ J I U~ I or -1 +/-UL3J..j I 'fDIL IIL IJ-'?¶ r'Lf rL 1744 I-7.fl Number of

of Live Original Young per 12 3 415 67 8

9 10 Young Adults Adults Adult 1 3. .~4 6T0' -7l 4 LI I 6 97 ir0 p2 7 __H If lI it a 1, Relicate Conc. 10.98% Outfall Conc. 22.0% Olutfall1 Replicate

o ubro Number of
oieOigia Young per 1o~v 2o 3Nu5inber8of(9 1

Init.Day____1 IAaUlts Aauit G0 CI- ~~- 6 6i 2ý;DŽ I (I0 C P.nlt-nt,- 8 9110 &. Ie I V %-/ I I AL0,10 I

of Number of

-'I -- -

of Original Ael,.I+ý Imt.

a) -- I -- I - I - I - VLa -TH I A

Client: TednessVa 7torty-Seuoyah i Location: Soddy Daisy* Tennessee St j Dates Samples Collected: la .*o I j Yai Tesf to e-T.i o o I I I

of Number of Relicate

-umber of

of Live Original Young per 1 2 3

4 5 6789 10 Young Adults Adults Adult Conc. Init. Day ,e,. 3 C-O 43.9% V05 4 (o -. 3 L tJ, Outfalla l5C 5 C 5 1*6 Q (0 ,5 V__ S 7 1:) al 6 1.5"C- )';-j 17-11. .1 3, Totalý I )..q "2-6"24 I'V-29FT 7.-* ZH2.. 7..4 14,, 3 10 ] .L Renlicate T - I - I - ¶ I - Number of Youn2

of Live Adults
of Original Adults I-I I

Number of Young per Adult Init. Day C*2 on--- L ~C o3 t N¢* 7 l 9 .b lS"! 3 !. I i

1. 7_.7.-

to _d TotarN T'L 7 Z')I 211221231 -Th I2 I 2I ) I I ' 1.

of Number of Replicate Number of
of Live Original Young per 1

2 3 4 5 6 7 8 9 10 Young Adults Adults Adult Conc. Int. Day.................... VcS2 2-100% V 61 4 - H 5 H -3 IL, Outfall gAo 5 Q O, " r" e 9 _L q s 41 iz-6 7 9ý r-InI *** 1.5,o t.. 5jt.5 7 15-15 g 6 O i-c 11 14 C1 q i 17-1 13* i.n 10 t3.5" 1 Tt-I L. L 2.-i '-f 1IL2.[3 I Total~i

2ý,*

10 1Q , -'. , - -*-.'. -- '-I--' Conc. 72% Outfall 4 15 I 2 13 6 7 8 9 10

iChe TneeeVley Authority-Sequoyah - Aystvs4 Location: Soddy Daisy Tennessee '7est Start-Date/Time: 0 ' Iw30 Dates Samples Collected: IJ2.ho, 12hi 2-h-4 Test Stop-Date/rime:'-1-1 iio~ I 5o r I 1 Re1ite 6w 1 C-1 0 jQ. _A 1-1---C 7 1~.L L 17 1 _1___.3 1otall? -32, 23 ItIt~ [2 201 IL22iJA 1 7&- Jf- JO £ A I Original Conc. 100% Intake i r, RpnliC.qti-

of Live Arhilte Young per A.4,,I+

Number of 2 3 4 -v, 0 1

3 .. 4 .""5 - 6 7 31.000 22.000 25.000 26.000 24.000 30.000 29.000 25.000 27.000 29.000 27.000 30:000 26.000 24.000.26.000 24.000 22.000 20.000 24.000 28.000 23.000 28.000 28.000 28.000 24.000 29.000 28.000 25.000 27.000 22.000 23.000 24.000 23.000 28.000 24.000 25.000 24.000 _28.000 24.000 22.000 -24.000 24.000 23.000 29.000 25.000 24.000 24.000 24.000 23.000 23.000 23.000 21.000 26.000 22.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 25.700 1.0000 25.700 22.000 31.000 10.384 10 25.725 1.0000 10.98 24.100 0.9377 24.100 20.000 28.000 9.672 10 90.00 75.00 25.725 1.0000 22 26.800 1.0428 26.800 23.000 30.000 10.376 10 116.00 75.00 25.725 1.0000 43.9 26.300 1.0233 26.300 24.000 31.000 10.147 10 112.50 75.00 25.725 1.0000 72 24.500 0.9533 24.500 22.000 29.000 9.475 10 88.50 75.00 24.900 0.9679 100 25.300 0.9844 25.300 21.000 30.000 11.187 10 101.50 75.00 24.900 0.9679 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.16764 1.035 0.25398 -0.8876 Bartletts Test indicates equal variances (p = 0.99) 0.63383 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Linear Interpolation (80 Resamples) Point SD 95% CL Skew IC05 >100 [CIO >1 IC15 >1 IC20 >1 IC25 1C40 >100 >100 I050 >100 50 100 150 Dose %

t4.

.,-. --. Page 1 D-Control 10.98 22 43.9 72 100 28.000 26.000 30.000 31.000 24.000 27.000 00 00 00 0.9 0.83 0.7 0.6 C 0.5. w 0.4. , 0.3 0.2 0.1 0.0 -0.1 0 + u 1.0 I

C-rfacl p-nia Survival and Reproduction Test-Reproduction U SatDate: jl,2IlO/0l 14:30 rstID: SNl201lncr jle ID: 6450TN02646O d Date: 4217I01 15:00.L-ab MD: 8&ME INC.-w ,anie EFF3,PovePI = Sample Date: .*Protocol: EPAF 91-EPA Feshwater sSpecies: .CD-Cenodaphn a dubia A Comments: Conc-% 1 2 3 4 5 6 7 8 9 10 D-Control 28.000 31.000 22.000 26.000 24.000 26.000 24.000 24.000 28.000 24.000 Intake 30.000 32.000 23.000 25.000 26.000 27.000 26.000 22.000 24.000 25.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 25.700 1.0000 25.700 22.000 31.000 10.384 10 Intake 26.000 1.0117 26.000 22.000 32.000 11.750 10 -0.234 1.734 2.224 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.93247 0.868 0.76037 -0.0034 F-Test indicates equal variances (p = 0.69) 1.31045 6.54109 Hypothesis Test (1-tail, 0.05) Homoscedastic t Test indicates no significant differences I

ca Eoean:. Teplare 4No.:-l*c Control: (20%DMW) 0 1 2 3 ' 5 '6! -Remarks T e m p e r a t u r e -i n i t i a l L -2"e- -m"a.. Final .4.32-11. l "D.O. Initial." Z Final TA -G. S0 z -/ pH Initial s i 7_

q1A*

Final 0 5 7 Rear Alkalinity 0 Il 6 Hardness Conductivity-Initial

-i 170 3j __

s Analyst Initials -79~ 1-75_ QA Review Initials e l 5 o l.so ".gc Day 0.98% OutfallO00 0 1 2 3 4 5 6 -Remarks Temperature-Initial s D.O. Initial r7-5i 1h-.77.. T v PH Initial 9.0o

g.

8 Conductivity-Intitial

g3

.l Q-u0? I 7[ Analyst Initials i9so , 1) k-- V, Li QA Review Initials LUm L., ru j.sO o.o L.) L_ i-.o Day 22% OutfallO001 0 .1 2 3 4 5 6 7Remarks Temperature-Initial (o4,-4.,da. ~S-2S.Ij~ D.O. Initial 1-7.1 7 pH Initial ~ Conductivity-Intitial M Le ec.fý Analyst Initials LS 2*i+/-. QA Review Initials --4) s ~ ~ L~k

-.,*.r*-.

,-'*'.* Page 1 ) S!*1

N o..' 43.9% OutfallO 00 0 1 2 3 -4 5 6 .7 --Remarks Temperature-Initial P41 a-4 -A___32S,____ 0 D.O. Initial '7.1 1* 7 Z~7 ~_ Final 1i 7_ E-qL Z& C___to pH Initial V'O ~-_9~___ Final ~_ Conductivity-Initial ý,ý Is" _________f Analyst Initials L'Ao DýK jp~ QA Review Initials LQ Li -J L. 2 L.-s r) so 72% Outfall 001 0 1 2 3E4 5 6 7 Remarks Temperature-Initial

H,(,

~ i5~,3 S._ I__ Final

0 9 25, J

~D~

D.O. Initial rii 5 Final ml -17 6.e -1'. 0 b-9 -1C U __ pH Initial 7~ -3 Final N 3 0 j___ Conductivity-Initial 19~D q i t3 -j~ Analyst Initials LS in ~ QA Review Initials "D LS r,-C, 100% Outfall 001 0 1 2 3 14 5 6 7 Remarks Temperature-Initial

N.qk 9 )A S. 1251 0 2Jc2 Final

-':-+i i-iSi 2 j0 Lf j D.O. Initial T.)o Z -7~~ '~ ~~A~W Final pH Initial 1.- 17. c u,6 Final eLD Alkalinity J, -A -1 ,Hardness -m1 Conductivity-Initial. )9

)

ýChlorine 0,0 - .CE =0O~1 Analyst Initials L51 04 (10v !) wdx QA Review Initials ý oL~oo5jo LSo uo Sample Date

.ah o(: 'o i

t 'Z412 1.i ~jz4 7I4 WJ Notes: Page 2

Daily Chemistry Data - Ceriodaphnia 7-day Chronic Client: TVA - Sequoyah Nuclear Plant Analysts: vw.,tSo,t Location: Soddy Daisy. Tennessee Dates/times: i, o, p+&,.- 1.:I o 1,7"0 Template No.: AMe of neonates: *_*L.? A* Intake-100% 0 1 2 3 4 5 6 7 Remarks Temperature-Initial

)q.3 o" )_._-q

_c 5,0 Final 4,4 ; .\\ ZI - Vc,. 2-2 24 D.O. Initial 7., 1 JZTT-Final 0 -b. .0 pH Initial J 1Z~ 79~ 7i'g 7fe -;2 Final &1O~ Alkalinity _-7 -- 0 Hardness -7;- -O 1./I Conductivity-Initial Io \\q-

t 2S

'i.3 n D I _q_ Chlorine Z I Analyst Initials IJ A. r. 4( F') V QA Review Initials

0.

n k_ -,Y) L~n q So f-ý, I "T Sample Date l-hd t3 -,, 1 'l k-' tz I4v1 tz Pi ) (?j IA Notes: a. Day Aze of neonates: Irs I t a ~ 3.= .7¸

Cerio Water Chemistry SQN-December 10 - 17, 2001 INITIAL AND FINAL WATER CHEMISTRY (CERIO) Initial Chemistry CERIO Control 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.6 24.3 24.4 25.2 25.1 24.0 25.0 24.0 25.2 24.7 DO 7.1 7.5 7.6 7.2 7.2 7.6 7.2 7.1 7.6 7.3 pH 8.1 8.1 7.9 7.9 8.0 8.2 8.1 7.9 8.2 8.0 Cond 181 179 173 170 134 150 159 134 181 164 Hard 90.0 90.0 95.0 90.0 95.0 91.7 AIk 67.0 62.0 60.0 60.0 67.0 63.0 Concentration 10.98% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.7 24.3 24.4 25.4 25.2 24.0 25.0 24.0 25.4 24.7 DO 7.1 7.5 7.5 7.2 7.2 7.8 7.2 7.1 7.8 7.4 pH 8.1 8.0 8.0 8.1 8.1 8.1 8.1 8.0 8.1 8.1 Cond 183 180 179 176 152 156 161 152 183 170 Concentration 22% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.6 24.5 24.5 25.2 25.1 24.0 25.1 24.0 25.2 24.7 DO 7.1 7.4 7.5 7.2 7.2 7.6 7.2 7.1 7.6 7.3 pH 8.0 8.0 8.0 8.1 8.1 8.1 8.1 8.0 8.1 8.1 Cond 185 183 182 182 160 159 160 159 185 173 Concentration 43.9% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.5 24.4 24.4 25.3 25.0 24.0 25.0 24.0 25.3 2 4.7 DO 7.1 7.2 7.5 7.2 7.2 7.5 7.2 7.1 7.5 7.3 pH 8.0 7.9 7.9 8.0 8.0 8.0 8.0 7.9 8.0 8.0 Cond 187 -186 187 188 168 168 169 108 188 179 Concentration 72% 0 1 2 3 4 5 6 MIN MAX MEAN 24.6 24.6 24.6 7.1 7.0 7.5 7.8 7.8 7.8 190 189 193 25.3 7.2 7.9 194 25.1 24.0 25.0 24.01 25.31 7.2 7.5 7.2 7.0 7.5 7.9 7.9 7.9 7.8 7.9 179 178 181 178 194 Concentration 100% 0 1 2 3 4 .5 6 MIN MAX 24.6 24.8 24.9 7.0 7.2 7.8 7.7 7.6 7.8 193 194 201 70.0 80.0 63.0 69.0 0.07 0.08 25.4 7.2 7.9 205 25.1 24.0 25.0 7.2 7.5 7.2 7.8 7.8 7.8 191 191 183 80.0 67.0 0.07 Page 1 of 3 Temp DO pH Cond Temp DO pH Cond Hard AIk TRC 24.71-7.2 7.91 1861 MEAN 7.0 7.8 7.3 7.6 7.9 7.8 183 205 194 70 80 76.7 63 69 66.3 0.07 0.08 0.07 4 24.01 25.41 24.8

Cerio Water Chemistry 0 1 2 3 4 5 6 MIN MAX 24.3 25.0 24.8 25.3 25.3 24.0 25.0 7.0 7.1 8.0 7.2 7.2 7.6 7.2 7.4 7.6 7.7 7.8 7.8 7.8 7.8 190 198 196 205 193 192 194 72.0 80.0 76.0 67.0 70.0 68.0 0.15 0.06 0.05 MEAN Final Chemistry CERIO Cerio - Medium 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.3 24.9 25.5 25.4 24.4 25.3 24.8 24.3 25.5 1 24.9 DO 7.0 7.8 7.0 6.3 6.8 6.9 7.5 6.3 7.81 7.0 pH 8.0 8.1 8.0 7.9 8.0 8.0 8.1 7.9

8. 1 8.0 Concentration 10.98%

1 - 2 3 4 5 6 7 MIN MAX MEAN Temp 24.4 24.8 25.4 25.4 25.2 25.0 24.6 24.4 25.41 25.01 DO 7.0 7.7 6.8 6.3 6.6 6.8 7.7 6.3 7.71 1 7.0 pH 8.1 8.2 8.1 8.0 8.0 8.0 8.1 8.0 8.2 .8.1 Concentration 22% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.4 24.7 25.4 24.7 25.2 25.0 24.6 24.4 25.4 1 24.9 DO 7.0 7.7 6.8 6.6 6.7 6.8 7.8 6.6 7.81 7.1 pH 8.1 8.2 8.1 8.0 8.1 8.0 8.1 8.0 8.21 8.1 Concentration 43.9% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.0. 24.6 25.3 24.3 24.7 25.2 24.5 24.01 25.31 1 24.71 7.1 7.7 6.8 6.7 6.9 6.9 7.6 6.7 7.7 8.1 8.2 8.1 8.1 8.1 8.1 8.1 8.11 8.21 1 Concentration 72% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.0 24.5 25.1 24.0 24.6 25.0 24.6 24.0 25.1 24.5 DO 7.1 7.7 6.9 7.0 6.9 6.9 7.6 6.9 7.7 7.2 pH 8.1 8.3 8.1 8.1 8.2 8.2 8.2 8.1 8.3 8.2 Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.1 24.4 25.0 24.0 24.5 25.0 24.4 24.01 25.01 1 24.51 7.0 7.8 6.9 7.1 7.0 7.1 7.6 6.9 7.8 8.1 8.3 8.2 8.1 8.2 8.2 8.2 8.1 8.3 Page 2 of 3 Intake Temp DO pH Cond Hard Alk TRC 7.0 8.0 7.3 7.4 7.8 7.7 190 205 195 72 80 76.0 67 70 68.3 0.05 0.15 0.09 DO pH DO pH 7.1 8.11 7.2 8.2 24.0 25.3 24 8

Cerio Water Chemistry 1 2 3 4 5 6 7 MIN MAX 24.2 24.1 24.9 24.9 24.3 25.2 24.6 24.1 25.2 7.0 7.8 7.0 6.7 7.0 7.0 7.7 6.7 7.8 8.1 8.0 8.1 8.2 8.2 7.3 8.2 7.3 8.2 MEAN 24.6 7.8 8.0 Test temperature Mean: 24.7 Min: 24.0 Max: 25.5 Word Review by: L.izý Excel.Reviewed by: 0 Page 3 of 3 Intake Temp DO pH

Sequoyah Nuclear Plant Biomonitoring December 10-17, 2001 Appendix D Reference Toxicant Tests and Control Chart Information

Fathead Minnow Potassium Chloride Chronic Reference Toxicant -"C.= w - -Control Chart Source: S&ME Inc.-Cultures Dates Dates Values Mean -2 SD +2 SD 6/13/00 0.6935 7/10/00 0.1400 0.4168 0.0000 1.1996 7/26100 0.7000 0.5112 0.0000 1.1541 8/24/00 0.7760 0.5774 0.0000 1.1653 9/17/00 0.7317 0.6082 0.0807 1.1358 10/17/00 0.7892 0.6384 0.1439 1.1329 11/30/00 0.7997 0.6615 0.1939 1.1290 12/30/00 0.7418 0.6715 0.2349 1.1081 1/29/01 0.3442 0.6351 0.1721 1.0981 2/19/01 0.6250 0.6341 0.1975 1.0707 3/6/01 0.8859 0.6570 0.2159 1.0981 4/9/01 0.8693 0.6747 0.2366 1.1128 5/10/01 0.7948 0.6839 0.2592 1.1086 6/4/01 0.7580 0.6892 0.2793 1.0992 7/18/01 0.8288 0.6985 0.2970 1.1001 8/20/01 0.8040 0.7051 0.3136 1.6966 9/18/01 0.7039 0.7050 0.3260 1.0841 10/30/01 0.8997 0.7159 0.3368 1.0949 11/18/01 0.7502 0.7177 0.3490 1.0864 12/9/011 0.7514 0.7194 0.3602 1.0785 2 etn Q Q, 1.5 1 0.5 0 4

Fathead Minnow Daffy Test Information Logsheet Test Name: Chronic Reference Test Test Dates: i1Io, - a-b. Daily Test Temperature Information Feeding Information est Initiation, ater information (25+/-1oc) (feeding interval 6e lha) Change, or Test in rmtln (5+/--C (eednginervl +/- h) Termination Control Water Carboy No. and DaeadIncubator Temp. Fed 100 uL Date and (or Therm. Be Feeding Additional comments Initials (OC) No. Brine time Start Time End Time aDa. a .m. Carboy #_Date Prep. DayO LW am: CU('a t O3 " 7_53 j __pm:,,Wo -56

i.

1-ihl Day I"l -"am:ILO 11 010 1~ 7-5tZ ~pm: 1 ~ ILA__ Day 2 am: b warn pm: 0 19 - l- ") Day 3 v;' a "m:

e.

DaySie '1 .a2.. im:C&eC ItO jj0h' r2.Ioi Day 6 "a m: icco 0©

I 12-/ 03t Day 4 7.

le~4~S It

4)

___)6_,12)1

Client: S&ME Ch Location: S&ME L a.-'7- " *,,.z *. .* D ates :. -. CUMULATIVE MORTALITY OF P. "-EMELA IN CHRONIC TEST REPLICATE DAY A B C D 1 I-r) 0' O10 0: 0 0 36 0 4 -C 5 T.-, 0 0 6 c 0 0 6 0

0) 0 7

C.) 0 3 b C) -1 4 C24o (Lr 2 0 c I c 3

6)

2.

1' 4 uO Ud.-iz! 6 F

3 7

I Ls 3 2' 4 (. (-7" (2' ( 4-G 6 S3

7 qC.

7 (, lot : j L LD A DAY CONC. Initials NO. 1 2 3 4 5 6 7 REPLICATE CONC. Initials NO. ronic Refer, \\XVS 2 £ 1500 mg/L \\Ct7 3 / (KCI) 4 0**q- .*IG 6 E -7 2 3 4 5 6 7 1 2 3 4 5 6 7 2 3 4 5 6 7 1 2 3 4 5 1-1 16_______ _______ 27 _ _ _ 3ii__ C.

FATHEAD MINNOW SEVEN DAY TEST j7 RAW DATA 7 SURVIVAL AND GROWTH Client: S&ME Chronic Reference Test Test Conducted By: D*7 " 6 .4-,,1 Z) I, -( Date Weighed bh1 ,Z1 / Weights Conducted by: b~... Oven Temp Test Dates:r*Lgd.-,*,./, (co, j'> Comments: Drying Time(h) ( 4dae/ti) me)Q 1'. End of drying (date/time)_L 6) 10 /2 L41>t,.d Groups Dry Pan Weight Exposure &

Uve
Original Tare Dry Weight (Pan + Fish Mean Repi.

Rep. Fish Fish Weight (mg) Pan + Fish (mg) minus Tare)(mg) Dry Weight (mg) Control A ic

J5C, 3~fLc

~ ~ (Mod. Hard) B 1A s Lý5s I SIq (3 C C,4ik~.E ..449 250 mg/L A __6____ ,O (KCI) B 500 mg/L A i k ~) (KOI) B/1t~~,7i 1Ag'ý 3 "Q c 1L' ./// _____D A4(2,,!&~ i k 750 mg/L A jý Af 0 -3O 67 3LLS5__ (KCI) B 4J A(I3 6-.~ŽL 4,( C D /AOk~ /~54,5 0 00, 4o-1000 mg/L A r3(15 2 Y E I'l _______1_11-7 (KCI) Bj / (6 /Y/c;/' 3_ "C "D Z( ý

v ý**O-

Larval Fish 0_-$V-S t Date:.

1i2J9/01 11:35.ý-Tjest ID0: Ref12011 'Endo Dt:.3/4/101 11:00 4Lýb D "Sample Date:. Protocol: EPAF Comments: Conc-mg/L 1 2 3 4 D-Control 0.5030 0.4530 0.4900 0.5270 250 0.5010 0.5010 0.4260 0.4990 500 0.4083 0.4320 0.4920 0.4610 750 0.3550 0.4110 0.3090 0.4090 1000 0.1710 0.1340 0.1300 0.2880 Transform: Untransformed 1-Tailed Isotonic Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean O-Control 04933 1 onnn n Qa'O n A937 n 250 0.4818 0.9767 0.4818 0.4260 0.5010 7.717 4 500 0.4483 0.9089 0.4483 0.4083 0.4920 8.077 4

750 0.3710 0.7522 0.3710 0.3090 0.4110 13.153 4

S

1000 0.1807 0.3664 0.1807 0.1300 0.2880 40.854 4

Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) Bartlett's Test indicates equal variances (p = 0.60) Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV Dunnett's Test 500 750 612.372 0.4933 1.0000 0.339 2.360 0.0800 0.4818 0.9767 1.326 2.360 0.0800 0.4483 0.9089 3.608 2.360 0.0800 0.3710 0.7522 9.222 2.360 0.0800 0.1807 0.3664 Statistic Critical Skew Kurt 0.94546 0.868 0.54046 0.49254 2.76513 13.2767 TU MSDu MSDp MSB MSE F-Prob df 0.07997 0.16213 0.0665 0.0023 6.7E-07 4,15 Linear Interpolation (80 Resamples) SD 95% CL(Exp) Skew 119.00 0.00 639.68 -0.2193 80.68 65.98 639.22 -1.5899 47.43 431.48 741.82 -0.1763 1.0 51.28 551.96 818.15 0.2887 39.48 600.04 821.49 -0.6644 0.9 26.11 770.29 920.45 -0.1432 0.8 0.7 O 0.6 00.5 S0.4 0.3 0.2 0.1 0.0 0 500 1000 Dose mg/L 1500 Point IC05 IC10 ICI 5 IC20 IC25 IC40 IC50 mgIL 348.47 514.25 593.98 673.71 751.40 848.62 913.44 pn I

_______________-~ -- ~-16 W% 5z4.za-I Control:fMod. Hard) 0 1 2 3 4 5 6 7 .-:,Remarks Temperature-Initial iiD _Lq_ Final 1 2.5s.o 2s.3 D.O. Initial -i.2 - 7.1 '7.3 1-7.S Final _1 -0 "0 4 1-7, 1 pH Initial "T 7.9 9ý 1.q I~ Alkalinity .- 71 UL" 2- --7 G Hardness 9-5 q9 cc) cR 4f, cl _ Conductivity 2i4

3_2,

ý31 2 WS Analyst Initials I "IL-5 -,ý- InID, ISý QA Review Initials

c.

L$D v po I) $uo )-S.0 LS ,;D Day 250 mg/L (KCl) 0 1 2 3 4 5 - 6 7 Remarks Temperature-Initial 2Z, q L `.m .o 2-4. C Z.o -Z 211 Z --ý). I Final 2_

Mt4.

D.O. Initial -73 7 -72..-7 Final to L. 1 "7._ pH Initial 2q 1 1 1

53.

__7__S Final 1M-7,tO- ~-SI ~-7 kl- __ Conductivity 37, Uct -- i -7__%_tom Analyst Initials VYV( QA Review Initials 5 L0 4.S' Is* _(r) L< ,) Day 500 mg/L (KCI) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 2,, j - J_+._7 P_5.L_2_3 _Q. Final 24, -2.5 A 2S,.L 2;.* ._._.,A D.O. Initial 7- -LI 7.2 -7. Final to -I - c"- 7 L-J2 1 pH Initial Final 7.(o-7,L (C -7 -1(o 1,7-X71 Conductivity j/6-I.L \\o2-7 IZUZ.2b2 1.J~*2 [Analyst Initials

4.

1$ L QA Review Initials L*-7 LSO -SPLSD L0* Do LSo US "iri"" r'%,- r t i ' INVIZ6: Paqe 1

Daffy Chemistry Data - Fathead Minnow 7-day Chronic Client: S&ME Reference Test Analysts: Ln b,"T Location: S&ME Inc. Laboratory Dates/times: P, p., /, Template No.: I a,,1,?¶y,. 750 mg. * (KC1) _..1 2 3 4 5 6 1 7 Remarks Temperature-Initial 1.. 2-"*0. 3 Final

5. 2 -'.' 5 s

, i. D.O. Initial 7Q U 7- _ _2-1--c------- ie Final 7_ 5-L pH Initial Final a 07-._ 17 L? 7.9 -7 Conductivity S s.l.

i. iS "3*

--71 Analyst Initials S \\ QA Review Initials LaD o5 L !j5o L*O Day 10_00 mg/L (KC1) 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 4 a4--, -Lf Final 3 2 5 2-5.

Z 1,

1. D.O. Initial -1. T. '7 U 7, -7 L Final -dko-"7.__L L 7 pH Initial Q-.-I-. I,*, ok Final s_ 1-7 Conductivity .t__y n 51' 1-5 3 Analyst Initials V - - QA Review Initials £ýs

i5 z)

I s-) &9 J-S) Da 1500mmg/L(KC1) 1 0 1 2 3 4 5 6 7 Remarks Temperature-Initial Z,6

4. 0 -a4.4 Q--

Final 26.'D 24A/ D.O. Initial I,7l L 7.3 /"---""-.__ Final U pH Initial ~ J? .HC____ Final h, T - Conductivity 2 4f -- 0 o )*7 Analyst Initials D [ vs QA Review Initials 1,,p I Ls LSVO L-S o Stock Solution Date !,ib,. 7o -,°, ,2,o iZ o_ NOTES: p~np 9

~yeroagpirnia dubia Sodium Chloride ChriiilcF -f-' i COfrI 'ChaR I CV%=10.6Source: S&.ME IcCutres Dats 0 Dates Dates Values Mean -2 SD +2 SD 5119/00 6/16/00 07/14100 8/11/00 09/29/00 10/20/00 11/27/00 12/18/00 1/15/01 2/23/01 3/14/01 4/17/01 5/20/01 6/15/01 7/17/01 8/14/01 9/24/01 10/29/01 11/27/01 12/10/01 Q In M Q I:- S 1.1038 ) 1.1100 ), 1.1106 1.1483 1.1180 0.9996 1.1931 1.2921 1.1200 1.3046 1.5233 1.0857 1.3621 1.2154 1.0089 1.2115 1.2183 1.1179 1.2857 1.0982 1.1007 1.0776 1.0829 0.9966 0.9946 0.9670 0.9759 0.9665 0.8994 0.8985 1 0.9053 0.9179 0.8993 0.9103 0.9205 0.9228 0.9310 0.9324 1.2857 1.1857 0.9310 1.4405 1.1171 1.1823 0.9324 1.4322 1.1069 1.1081 1.1182 1.1181 1.0984 1.1119 1.1344 1.1328 1.1500 1.1839 1.1758 1.1901: 1.1919 1.1797 1.1817 1.1838 1.1802 1.1857 1.1823 1.1156 1.1156 1.1588 1.1533 1.2002 1.2292 1.3019 1.2897 1.3336 1.4685 1.4530 1.4749 1.46659 1.4601 1.4531 1.4472 1.4376 1.4405 1.4322 -- ww

.JODAPH TEST INFORMATION SHEET Test Name: Chronic Reference Test Test Conducted From: *Ao o (DayO)To lii 1 (Day7) Sites/Concentrations: I.4. 2.. Sa-'o -,*_.

5. -'t-o r

3.

r-, L 6 Stock (if applicable): 0 O - _ c _ l -t* Control Water Type (/): 20% Dilute Mineral Water + Trace Minerals Other (describe): __ Dilution Water Type (/): 20% Dilute Mineral Water + Trace Minerals Other (describe):_ E Source of Test Organisms: S&ME brood board nos. 1 Age of Test Organisms: Isolated neonates for test on,-I-lo 1o1 (date) from Q 0 30 to -L4 ec (time) Initials. ( Record of Minor Test Non-Conformity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: rest Log sheets QA Reviewed.By: Rei Initials) on 13q (Date) /E.1 auz 0set REV.O I, January 00, Ssheet)

1 cCeriodaphnia Daily Test Information Logsheet est Name: Chronic Reference Test Test Dates: ._Ao, Daily Test Temperature Information information (cup -25+/- 1C) Feeding Information Date and Incubator Temp. (0C) Therm. Fed 100 uL Fed 100 uL Selanastrum Initials No. YCT Selenastrum Date a.m. p.m. D a y O 1 L *, Day 0 Day I [2I~g'*, 2-' J.'* 53q o '7l,.i* 16-3o Ill) ,)i> Day 7V_6 est nitiation, Water Change, or Test Termination Control Water Carboy No. and Additional comments Start Time End Time Carboy # Date Prep. -I ix 1 -4% 1I-'OO 16-3b I -llIalo) I -- + 3c I,rc I rlo q3 63 I lzlc1 n!,) . 1,I?'

, M;!

Loc~ Location: S&NMEInc Analysts: I-SO 12 3 .Day 2 3 4 Conc. Init Control Replicate 4 516I7T8 9-210,---' No. of No. of live No. of Young Per Young Adults ut. Adult.I 1

~1LJLJi~~

to C7,: TTTtR-I i- Replicate No. of No. of live No. of Young Per 15 6 17 9 10 Young Adults Original Adults Adult I 1 / !- "- J j C/ I..-.' N JkI I I OIt'nfl I -T "ir, Jeplicate No. of No. of live No. of Young Per 5 6 7 8 9 10 young Adults Original Adults Adult I I I I 12 4 4 f-I. + - .1 L4I5I4O I C. 1 2.1 3 4 I~nit.1 Day - Ao2 .0 500 :LO3 C. D 7 J* O* Total.%" i ba 1 2 3 41 Init-Day L 2 o2 750 3* s YS4 z+/- '3"2 Totl_; - b" 4 b 4a1,", l 1L* 4. z 1 r -

v.

I *,

I IL I I. J

a-H 7

6 vt o l 1 7 G: 6 i I Totalg$.3I J I.(') l (*)" t t No. of live: 1 nl YoungPe I' J4 No. of ()I OI-Z M' I0 0 ,('D / r,. S I l,w** I ¢"*. w Io A4 57 0-I1. S*l il I11

Cliet: Referec t Location: Analysts: 'g, w.s. 4 PaaR 2

!.End Date: 7i2/17101 ,30 ý-sarrnýe Date:i. ~ PItOc-EPAF 91-EPA Frs tr os Sacis 'd i u ia Comments: = Conc-mg/L "1 -*2 3 -4 ~ .6~~7 -=8 ~ 10 D-Control 24.000 23.000 26.000 24.000,25.000 26.000 27.000 27.000.22.000 24.000 500 25.000 29.000 26.000 20.000 '22.000 27.000 22.000 26.000 26.000 26.000 750 22.000 27.000 24.000 22.000 27.000 27.000 26.000 24.000 25.000 25.000 1000 23.000 19.000 19.000 17.000 21.000 23.000 20.000 21.000 20.000 --22.000 1500 16.000 16.000 12.000 12.000 18.000 14.000 13.000 17.000 16.000 17.000 2000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 Transform: Untransformed Conc-mg/L Mean N-Mean Mean Min Max CV% N D-Control 24.800 1.0000 24.800 22.000 27.000 6.801 10 500 24.900 1.0040 24.900 20.000 29.000 10.949 10 750 24.900 1.0040 24.900 22.000 -27.000 7.679 10 "*1000 20.500 0.8266 20.500 17.000 23.000 9.270 10 -0

1500 15.100 0.6089 15.100 12.000 18.000 14.459 10 2000 0.000 0.0000 0.000 0.000 0.000 0.000 10 1-Tailed Isotonic t-Stat Critical MSD Mean N-Mean 24.867 1.0000

-0.106 2.223 2.100 24.867 1.0000 -0.106 2.223 2.100 24.867 1.0000 4.552 2.223 2.100 20.500 0.8244 10.268 2.223 2.100 15.100 0.6072 0.000 0.0000 Auxiliary Tests -Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.95611 0.93 -0.3933 -0.5821 Bartlett's Test indicates equal variances (p = 0.65) 2.45569 13.2767 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 750 1000 866.025 2.10037 0.08469 186.28 4.46222 1.3E-14 4, 45 Unear Interpolation (80 Resamples) Point mg/L SD 95% CL Skew IC05 821.183 69.9968 681.554 fn1 -o A 7n71 IC10 892.366 30.9724 820.33 926.357 0.8830 IC15 963.55 35.013 891.735 1022.56 1.1642 IC20 1056.17 46.035 960.829 1116.43 0.4145 IC25 1171.3 46.0524 1060.1 1 1237.08 -0.1350 IC40 1505.96 39.2483 1396.43 1541.33 -1.1204 IC50 1588.3 19.4053 1547.28 1617.78 -0.4628 c 0.5 S0.4

0.3 0

500 1000 1500 2000 2500 Dose mg/L 4

l,, t.=*.. L _IO c Lo n:ion S Template No.i:-:3/4-) Control: (20% DMW) 0 1 2 Ternerature-Initial O.* D.O. Initial

Davi-34 5II I

I I 'I c~idI*~ T Final

f.

14 2+/-L-__ pH Intial Final ,O0 Alkalinity (q Hardness C 0 / I Conductivity Me 1_71 Analyst Initials -SO L-,O = I QA Review Initials L ) 0_.mg/L _0 1 2 3 1-4 5 6 7 Remarks Temperature-Initial! -Z z F____I F in al A 74"-- D-0. initial 'L 4 -~ ~ ~

S0_______

PH Initial I Igo

o Final C onductivity

_7 . _,I Analyst Initials LSD) -.SO Lso7--*--__-__--__-_*__.S QA Review Initials L"o ý0', 750 mg/L 0 1 2 3 4 5 6 7 Remarks Temperature-Initial L L Final 1 v, 5 D.O. Initial j Final __'1 o_ nH Tnititn I 20f L 60" kj-Con Anal QA. Final t7C qZ.L 4 a ductivity 0- _ *_* j I [yst Initials L S D1 k-)? I -~ ~ W LQ Q) IS I LW~ a .4 7-512-1 Z4 7i-. J..** L' v .UULLLQaL -111.1"l C) i m I 3 4 F 2-61 2W. 1&1ý4)7,54 I

Client: Refrenc Location: S&Nf Template No.: I 1000 mg/L _R g ft-.....V ily Chemistry Data - Ceriodaphnia 7-day Chron -ic Test Analysts: u--

c.,

-Inc. Dates/times: Age ofNeonates: <-:4W-.' D ay__________________ C o n d u c tiv ity l q.. Z " L.i) Analyst Initials ao )""*"o" .QA Review Initials LSO o

0 O

I l500 mg/L 0 1 2 3 4 5 6 7 Remarks Temperature-Initial '.3.--

1 27_.* ) 2, (, m QA Review Initials

,p

o F

), 2000 m.... L 0.. 1 2* 3 _4 5 6 7 Remarks D.O. Initial ,H Intial '4Rmm 0 If I'/ C7-O Fin~-al 15+ 0-9i / ,Conductivity O / / -tvyc,_I 'Analyst Initials D.s tot~ QlA Reie Intil I-s L-Stock Sol vv iub.LL4It L-SO L60 tJfl La&.. IIio3i I fj'' I - -.-- ' I 30 114110" 0 1 2 1 Day 3 14 5 6 7 Rf-TnnrvC I

Sequoyah Nuclear Plant Biomonitoring December 10-17, 2001 Appendix E Invalid Fathead Minnow Toxicity Test Bench Sheets 4 1

TOXICITY TEST RESULTS (See Appendix E for Bench Sheets)

1) Results of a Pimephales (Genus)

Conducted 12/10/21 (mm/dd/yy) promelas (Species) Chronic 7-d Toxicity Test (Type/Duration) 12/17/01 Using Effluent From Outfall 10_1. (mm/dd/yy) Percent Surviving Test Solutions (time intervals used - days) { 2 3 4 5 6 7 Control 97 95 95 95 95 92 92 10.98%Effluent 100 100 100 100 1O0 95 90 22.0% Effluent 100 100 100 98 93 85 80 43.9% Effluent 100 100 98 90 80 73 68 72.0% Effluent 100 100 100 88 88 85 83 100% Effluent 100 100 100 95 75 70 70 Intake 100 o00 88 60 48 43 40 IC2 5 Mean Dry Weight (mg) Test Solutions (Replication) 1 2 3 4 Mean Control 0.691 0.719 0.675 0.749 0.709 10.98%Effluen 0.894 0.633 0.528 0.865 0.730 22% Effluent 0.677 0.466 0.909 0.516 0.642 43.9% Effluent 0.622 0.150 - 0.539 0.651 0.491 72% Effluent 0.3 78 0.819 0.612 0.607 0.604 100% Effluent 0.631 0.757 0.131 0.461 0.484 Intake 0.043 0.333 0.142 0.591 0.277 IC25 Value: 75% Calculated TU Estimates t 1.3 TUc 95% Confidence Limits Permit Limit: 2.3 TUc Upper Limit = NA Lower Limit = NA NOTE: TUa = 100/LC50, TUc = 100/IC 25 REFERENCE TOXICANT TEST RESULTS Species Date Time Duration Toxicant Results (LC 50/IC 2 5) Pimephales promelas 12/09/01 1135 7 days KCI 0.751 mg/L KCI 4 (number)

Water Chemistry Mean Valuesand Ranges fbr Fathead Minnow Test, Sequoyah Nuclear Plant (SQN), December 10 - 17, 2001 Temperature Dissolved Oxygen pH Conductance Alkalinity Hardness Total Residual Test/ Initial Final Initial Final Initial Final Initial Initial Initial Chlorine Sample ID (°C) (oC) (rng/L) (mg/Lt) S.U. S.U. (mg/hos) mg/L CaCO3 mg/L CaCO 3 (mg/L) Fathead/ 24.6 24.7 7.3 7.0 8.0 7.6 325 66.4 94.6 Control (24.1-25.3) (24.0-25.3) (7.2-7.7) (6.5-7.9) (7.9-8.2) (7.5-7.8) (319-338) (62-70) (90-100) Fathead/ 24.7 24.8 7.3 7.1 8.1 7.5 317 10.98% (24.1-25.2) (24.0-25.3) (7.2-7.6) (6.6-7.7) (7.9-8.2) (7.4-7.8) (311-325) Fathead/ 24.7 25.1 7.3 7.0 8.1 7.5 304 22.0% (24.1-25.3) (24.9-25.3) (7.2-7.6) " (6.6-7.6) (7.9-8.2) (7.4-7.7) (298-311) Fathead/ 24.8 24.9 7.3 6.7 8.0 7.5 273 43.9% (24.0-25.4) (24.0-25.7) (7.2-7.7) (6.4-7.6) (7.9-8.1) (7.3-7.6) (269-277) Fathead/ 24.9 24.9 7.4 6.8 7.9 7.7 234 72.0 % (24.0-25.2) (24.0-25.6) (7.2-7.9) (6.5-7.6) (7.7-8.0) (7.4-8.0) (225-242) Fathead/ 24.7 24.7 7.4 6.9 7.7 7.5 197 66.3 76.7 0.07 100.0 % (24.0-25.2) (24.0-25.2) (7.2-8.0) (6.5-7.9) (7.6-7.8) , (7.4-7.9) (191-205) (63-69) (70-80) (0.05-0.08) Fathead/ 24.8 24.9 7.4 7.1 7.7 7.6 196 68.3 76.0 0.09 Intake (24.1-25.1) (24.5-25.2) (7.0-8.2) (6.7-7.7) (7.5-7.8) (7.3-8.2) (190-206) (67-70) (72-80) (0.05-0.12)

FATHEAD .05 Test Name: TVA-Sequovah Nuclear Plant Test Conducted From: _ I I,)- _(Day 0) To ij. 1oi (Day 7) Sites/Concentrations:

1. 0.*

fi

4. L4 3,9 Y,

2. ag9, qS

5.

3.

6.

Stock (if applicable): Control Water Type (V): Moderately Hard Water + Trace Minerals Other (describe): 0_ Dilution Water Type (V'): Moderately Hard Water + Trace Minerals Other (describe):___ Source of Test Larvae (V'): S&ME L)4-[ Other (describe):___ Date Larvae Hatched: \\a - 1;itde,;i Hatch Time: I(0o- - (-'9c Initials: L', Record of Minor Test Non-Conformity Date: ___ Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Revieweld jBy: 'A.. Rvee ntas n1. -(ae REV.01, JANUARY00 (fathead test info)

Client: TVA/Seguoyah Nuclear Plant Location: Soddy Daisy. Tennessee Analysts: 6A

2,i1CSb Dates:*

/1

7-7 CUMULATIVE MORTALITY OF P, PROMELAS IN CHRONIC TEST REPLICATE DAY REPLICATE DAY CONC.

Initials NO. A B C 2k 1 1 Mod. 12 3 L Q / / Hard ýE6 4 I-SO 5 L Q 2 '-2Z -* L ;Z, 6 1 7 f~ 2 (*2' C.) 10.98% 4i 4 c 6 -s Outfall 5 c ) 4,.-6 C c L 9z..&-h 2 02 0 -0 22.0% '4o 4 O C) Outfall 5 7 ALi2. 6 7 2 T. T j 1 0 0 c_ 2 0 43.9% / 4 7F 4 14 Outfall Lao 5 5 i 2 © /A LI 6 i -7 2, / 7 '= £.

2.

/ -7 -2 P*,-? 1 0' C, 0 6-4,n 2 r /,./ 3 ____j 'L-2 (, 72% p-b 4 Q Outfall LA 0 5 ,7-4 ,k* 6 6 / -f 45 A Th.. l -c AA-c.. z&s cN-1-leA 2 -O -7 100% 4 0 0 Outfall L.,O 5 C) 0 '7 3 , -LI' 7 6-g_?At-o 1

0.

C") C) C i-2 0 C_ 0 Q 100% P*-* 3 C-Intake /

r 4

"5" z 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 7 5U~. S,A CONC. Initials NO. w

FATHEAD MINNOW SEVEND AYIEIr R A W D A T A SURVIVAL AND GROWTH Client: TVA - Sequoyah Nuclear Plant Test Conducted By: 'vC s ý -s.,-s DateWeighed jAit-1 I Weights Conducted by: N-k-L5 Oven Temp T est D ates: 1 - l t* t (Q C L.L t Comments: Began drying (date/time) I; h r-7

1 c 1 Ic' v

,, u v uy* u L etimej t --x H. 3 c, i n Groups Dry Pan Weight Exposure &

Live
Original Tare Dry Weight (Pan + Fish Mean Repl.

Rep. Fish Fish Weight (mg) Pan + Fish (mg) minus Tare) (mg) Dry Weight (mg) Control A C,'f~ ~1~f.~ 9 (,q) Moderately Hard) B (, I q - L D 53;h 4~(~C 9~<~I 10.98% A c7 .5( '7 ~i q___ Outfall 101 B -7 I 4"7.*,. 6. 0 .L ..3 D J__ kD1 ~ 22.0% A 4. L 4 <Y 7 V 7* Outfall 101 B to L .G k L 7,'7 C Ic )A(,4 7Lf 1 D7Lt c.rC o 7. I A u "~ 7.C G O K _) * *. 76. _, 43.9% A S"" j, ZCf-, Dc Outfall 101 B ,o ) 4L 4, -2.) ) q.*,, -t L iL.'.... 1S OD _1_ / I ,PT

L 6 I

_) _ _,1 72% A 0, 31 Outfa ,l010 B g0 V, b,S 7 ' ) *4 , q _ ,11 10 0 % A J,9 7 0 2 0 \\-, (c -.3 I ,/__ Outfall101 B ,O 1.s 1 EH -v 1 S,.*p. 1 D C 3 I,- O 1 a I)Q-4-l.9 J-, 1. 5 ). 1 .L O__ D7,

) G q

) 3*.* "*,i O , I-I,.

FATHEAD MINNOW SEVEN DAY TEST FRAWDATA SURVIVAL AND GROWTH ~Oven Temp Client:TVA - Seguoyah Nuclear Plant Test Dates: Oiven I oj ( T0)

e Test Conducted By: \\

S < i,. "(.... L -. Com m ents: _ _ _ _ _ _ _ _ _ _ _ _ Date Weighed. L ix1 Began drying (date/time) )l')b i !, m Weights Conducted by: 0 1,,. -- '-C--- S.... *.. IEnd of dryingq (date/tim e) i t

o,/ L4,:

Groups Dry Pan Weight Exposure &

Live
Original Tare Dry Weight (Pan + Fish Mean Repf.

Rep. Fish Fish Weight (mg) Pan + Fish (mg) minus Tare) (mg) Dry Weight (mg) A If! ,) ,) g"(J -u* Intake B IQ ý , 9.7-7 7 aC.I o 3.-'3 100% C IC-t , 7 i.;5 ,4 D I__D C) f -47,~ Q,01i L/.X ______I A B C D A C D A B C D A B C --- __4 C D

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 12/10/01 14:00 Test ID: SQN1201fg Sample ID: TN End Date: 12/17/01 14:35 Lab ID: S&ME Inc. Sample Type: EF Sample Date: P'rotocol: EPAF 91-EPA Freshwater Test Species: PP Comments: Conc-% 1 2 3 - 4 D-Control 0.6910 0.7190 0.6750 0.7489 10.98 0.8940 0.6330 0.5280 0.8650 22 0.6770 0.4660 0.9090 0.5160 43.9 0.6220 0.1500 0.5390 0.6510 72 0.3780 0.8190 0.6120 0.6070 100 0.6310 0.7570 0.1310 0.4160 0026450-NPDES Permit # F3-Power Plant -Pimephales promelas Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.7085 1.0000 0.7085 0.6750 0.7489 4.588 4 0.7192 1.0000 10.98 0.7300-1.0304 0.7300 0.5280 0.8940 24.420 4 -0.154 2.410 0.3365 0.7192 1.0000 22 0.6420 0.9062 0.6420 0.4660 0.9090 31.070 4 0.476 2.410 0.3365 0.6420 0.8926 43.9 0.4905 0.6923 0.4905 0.1500 0.6510 47.280 4 1.561 2.410 0.3365 0.5472 0.7609 72 0.6040 0.8525 0.6040 0.3780 0.8190 29.828 4 0.748 2.410 0.3365 0.5472 0.7609 -0 100 0.4837 0.6828 0.4837 0.1310 0.7570 56.659 4 1.609 2.410 0.3365 0.4837 0.6726 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.96236 0.884 -0.4075 -0.4154 Bartlett's Test indicates equal variances (p = 0.16) 7.96597 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.33653 0.47501 0.0443 0.039 0.37737 5, 18 Linear Interpolation (80 Resamples) Point SD 95% CL(Exp) Skew IC05 16.111 7.134 0.000 34.339 0.5410 IClO0 21.242 0C15 29.084 IC20 37.396 0C25 75.449 0C40 >100 1050 >100 P m j\\ )A -y C>3C 0.6 - 0.5 0 0.4 S0.3 0.2 0.1 0.01 .0.1. 0 50 100 150 Dose % Page 1 ToxC(I.- vc n IC10 21.242 IC15 29.084 IC20 37.396 IC25 75.449 IC40 100 IC50 100 Ci. 'f,1 "", "/.

SQN DSN101 Dose Response Curve Chart 1 Sequoyah NP, DSN101, Fathead Growth 0.80 0.70 0.60 0.50 1 0.40................ 0.30 0.20............................. 0.10 0 10.98 22 Efflui Page 1 43.9 72 ent Concentration 100 .,.3 -7.3

Larval Fish Growth and Survival Test-7 Day Survival Start Date: 12/10/01 14:00 Test ID: SQN1201fs Sample ID: TN0026450 End Date: 12/17101 14:35 Lab ID; S&ME INC. Sample Type: EFF3-Power Plant Sample Date: Protocol: EPAF 91-EPA Freshwater Test Species: PP-Pimebhales oromelas Comments: Conc-% 1 2 3 4 D-Control 0.9000 0.9000 0.9000 1.0000 10.98 1.0000 0.9000 0.8000 0.9000 22 0.8000 0.7000 1.0000 0.7000 43.9 0.8000 0.2000 0.8000 0.9000 72 0.5000 1.0000 0.9000 0.9000 100 0.9000 0.9000 0.3000 0.7000 Transform: Arcsin Square Root 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD 0-Control 0.9250 1.0000 1.2876 1.2490 1.4033 5.992 4 10.98 0.9000 0.9730 1.2543 1.1071 1.4120 9.935 4 0.193 2.410 0.4160 22 0.8000 0.8649 1.1254 0.9912 1.4120 17.662 4 0.940 2.410 0.4160 43.9 0.6750 0.7297 0.9817 0.4636 1.2490 35.836 4 1.772 2.410 0.4160 72 0.8250 0.8919 1.1739 0.7854 1.4120 23.013 4 0.659 2.410 0.4160 -0 100 0.7000 0.7568 1.0172 0.5796 1.2490 31.069 4 1.566 2.410 0.4160 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.91089 0.884 -0.9339 -0.58272 Bartlett's Test indicates equal variances (p = 0.23) 6.93356 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.33618 0.36464 0.06107 0.0596 0.43258 5, 18 4. ToxCalc v5.0 Page I,

ON4. I= -tart Larval Fish Growth and Survival Test-7 Day Survival tart Date: .-12110101 14:00 ,,Test ID: SQN 12011fs

mple ID.

Pem <Ž-; End .at:-,.:- 1170143 1: l2.Oi..f - :-.-.-,,--~,~ +,,*, p ,*.-TN0026450-NPDES Permit # :--= ,.:+ End Dame: 701*:35 Lb I-* -ME Inc. Sample Type:. EFF3-Power Prant Sample Date: EPAF 91-EPA Freshwater +Test Species: - PP-Pimephales promelas ' Comments: Conc-% 1 2 3 4 D-Control 0.9000 0.9000 0.9000 1.0000 Intake 0o.1000 0.4000f n onnn n onnn Conc-% Mean N-Mean Mean D-Control 0.9250 1.0000 1.287

Intake 0.4000 0.4324 0.679.

Transform: Arcsin Square Root Min Max CV% 6 1.2490 1.4033 5.992 8 0.3218 1.2490 59.994 1-Iailed t-stat rrfif-nl N MCrn 4 2.929 1.943 Auxiliary Tests Shapiro-Wilk's Test indicates normal distribution (p > 0.01) F-Test indicates equal variances (p = 0.02) Hypothesis Test (f-tail, 0.05) Homoscedastic t Test indicates significant differences Page 1 k~al Sew K~u r U.010-10 41.Z1.23279 2.886678 ToxCIln v5 n -4 0.4033 1.23279 2.88667 N RR*F* =

-,I l *.11 j i* [ i 1-l I+I*

i 0.749

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'LLarval Fish Growth and Suivl

`.':Start Date: :.-`12I10I01 14:00 "Test ID: SQNl201nf SEnd Date: k%2/17/01 14:35 tab ID: S&ME lnc. ..Sample Date: M.E 9;1'-EPAo shwatr Comments: EPAF 91-EPA Frea Conc-% 1 2 3 4 D-Control 0.6910 0.7190 0.6750 0.7489 Intake 0.0430 0.3330 0.1420 0.5910 Transform: Untransformed Conc-% Mean N-Mean Mean Min Max CV% N D-Control 0.7085 1.0000 0.7085. 0.6750 0.7489 4.588 4

Intake 0.2773 0.3913 02773 0.0430 0.5910 87.042 4

1-Tailed 3.542 2.353 0.2865 Auxiliary Tests Statistic Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92908 F-Test indicates unequal variances (p = 8.03E-03) 55.1143 Hypothesis Test (1-tail, 0.05) Heteroscedastic t Test indicates significant differences Page 1 C*ritical 0.749 47.4683 47.4683 Skew Kurt 07408 2.0285 0.7408 2.0285 ToxCalc v5.0

Fish Water Chemistry SQN-December 10 - 17, 2001 INITIAL AND FINAL WATER CHEMISTRY (FATHEAD) Initial Chemistry Fish - Control 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.3 24.1 24.3 25.1 25.3 24.1 25.0 24.1 25.3 24.6 DO 7.2 7.4 7.7 7.2 7.2 73 7.2 7.2 7.7 7.3 pH 8.0 8.0 7.9 7.9 8.1 8.2 8.1 7.9 8.2 8.0 Cond 325 325 320 321 319 338 327 319 338 325 Hard 98 100 98 92 90 92 92 90 100 94.6 AIk 62 67 65 67 67 70 67 62' 70 66.4 Concentration 10.98% 0 1 2 3 4 5 6 MIN MAX MEAN Temp ,M I ')A q ')A Q ,) r-o 40 1 /--. I ý0 7.2 7.2 7.2 8.2 8.2 8.1 317 324 325 24.1 25.2 24.7 7.2 7.6 7.3 7.9 8.2 8.1 311 325 317 Concentration 22% 0 1 2 3 4 5* Il I" IVI"-1kMIN Temp 24.3 24.5 24.9 25.1 25.3-24.1 25.0 24.1 25.3 24.7 DO 7.2 7.3 7.6 7.2 7.2 7.3 7.2 7.2 7.6 7.3 pH 8.0 8.0 7.9 8.0 8.2 8.2 8.1 7.9 8.2 8.1 Cond 301 304 298 300 305 308 311 298 311 304 Concentration 43.9% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.5 24.5 25.1 25.4 25.0 24.0 25.0 24.0 25.4 24.8 DO 7.2 7.2 7.7 7.2 7.2 7.5 7.2 7.2 7.7 7.3 pH 7.9 7.9 7.9 8.0 8.1 8.1 8.1 7.9 8.1 8.0 Cond 273 277 270 274 274 269 271 269 277 273 Concentration 72% 0 1 2 3 4 5 6 MIN MAX MEAN Temp 24.7 25.0 25.2 25.1 25.0 24.0 25.1 24.0 25.2 24.9 DO 7.2 7.3 7.9 7.2 7.2 7.5 7.2 7.2 7.9 7.4 pH 7.8 7.9 7.8 7.7 8.0 7.9 8.0 7.7 8.0 7.9 Cond 235 225 242 229 238 235 237 225 242 234 Concentration 100% 0 1 2 3 4 5 6 MIN MAX MEAN 0 3 4 5 AINI NiAAY V.A~ T -r, i a~- DO pH Cond Hard Alk TRC 7.2 7.6 197 70 63 0.07 /-,+. 7.2 7.6 195 8.0 7.7 204 80 69 0.08 2z.0U 7.2 7.8 205 25.0 24.1 25.1 7.2 7.7 7.2 7.8 7.8 7.8 196 192 191 80 67 0.07 Page 1 DO pH Cond 7.2 8.0 314 7.3 8.0 315 7.6 7.9 311 7.2 8.1 313 7.6 7.8 7.7 191 205 197 70 801 76.7 63 69 66.3 0.05 0.08 0.07 I*/I Ilkl ItlAY 24.01 25.2 7.21 8.0 24.7 7.4

Fish Water Chemistry Intake Temp DO pH Cond Hard AIk TRC Final Chemistry FISH Fish - Medium Temp DO pH 0 1 2 3 4 5 6 24.2 25.0 25.1 25.1 25.1 24.1 25.0 7.0 7.1 8.2-7.2 7.2 7.7 7.2 7.5 7.6 7.7 7.7 7.8 7.8 7.8 190 199 197 206 196 193 194 72 80 76 67 70 68 0.15 0.06 0.05 1 2 3 4 5 6 7 24.4 24.7 25.3 24.8 24.4 25.2 24.0 6.5 7.1 6.8 7.1 7.0 6.6 7.9 7.6 7.7 7.6 7.8 7.5 7.6 7.5 MrN MA NAP A NT\\ 25.1 8.2 7.8 206 80 70 24.1 24.8 24.8 7.4 7.7 196 76.0 68.3 0.05] 0.12111 0.09 MIN MAX MEAN 24.0 25.3 24.7 6.5 7.9 7.0 7.5 7.8 7.6 Concentration 10.98% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.1 24.8 25.3 24.8 24.6 25.3 24.0 24.0 25.3 24.8 DO 6.6 7.4 7.1 7.0 6.8 6.8 7.7 6.6 7.7 7.1 pH 7.8 7.5 7.6 7.5 7.4 7.5 7.5 7.4 7.8 7.5 Concentration 22% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 25.0 25.0 25.3 25.1 24.9 25.3 24.0 24.9 25.3 25.1 DO 6.6 7.4 6.9 6.8 6.8 6.7 7.6 6.6 7.6 7.0 pH 7.7 7.4 7.5 7.5 7.4 7.5 7.6 7.4 7.7 7.5 Concentration 43.9% 1 2 3 4 5 6 7

MIN, MAX MEAN Temp 24.9 25.2 24.9 25.7 24.4 25.2 24.0 24.0 25.7 24.9 DO 6.5 7.0 6.7 6.5 6.4 6.5 7.6 6.4 7.6 6.7 pH 7.6 7.5 7.5 7.6 7.3 7.4 7.6 7.3 7.6 7.5 Concentration 72%

1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.9 25.0 25.0 25.6 24.5 25.3 24.0 24.0 25.6 24.9 DO 6.5 7.2 6.6 6.6 6.7 6.5 7.6 6.5 7.6 6.8 pH 7.6 7.4 7.5 7.6 7.9 7.8 8.0 7.4 8.0 7.7 Concentration 100% 1 2 3 4 5 6 7 MIN MAX MEAN Temp 24.9 24.5 24.8 25.1 24.5 25.2 24.0 1 24.01 25.21 1 24.77 6.7 7.3 6.7 6.7 6.8 6.5 7.9 7.4 7.4 7.5 7.7 7.4 7.4 7.9 6.5 7.9 6.9 7.41 7.91 7.5 Page 2 a DO pH MIN MA M*AM 24.1 7.0 7.5 190 72 67 MIN MAX MFAN

Fish Water Chemistry 1 2 3 4 5 6 7 24.9 24.5 25.0 25.1 24.7 25.2 24.6 6.7 7.4 6.7 7.1 7.1 7.0 7.7 7.6 7.5 7.6 7.7 7.3 7.3 8.2 MIN MAX 24.51 25.2 6.71 7.71 Test Temperature Mean 24.9 Minimum 24.0 Maximum 25.7 Word Review by:__ Excel Reviewed by: Page 3 I nta ke Temp DO pH MEAN 24.9 7.1

7.6 Client

TA-Location: S y Template No.: Paum NTry.' Y,- a t e Control: (Mod.Hard) 0 1 I2 13 -4 15 6 7- -Remarks Temperature-Initial

5

-~SJi 153 ;,. 0 Final 21,' 3 ________4-__2Sz i~ D.O. Initial r A 7-_.1 7-7E "7-5 72 Final 7 pH Initial ,o _Z .e.4. Final 1 .7 -1 "7._6 ! Alkalinity T_ U-Hardness gi -z-Conductivity-Initial 3*

Analyst Initials
j." jC

¢a.& , a QA Review Initials L-*o K&-n O 4o ýýO LEO "z Dw Da_ 10.98% Outfall 001 0 12 3 4 5 6 7 Remarks Temperature-Initial Q "Z2'",A Final 0 2*.- D.O. Initial 7-. 7" L 2 -7.1 '7 Z Final l7_ TL -2 L,-, 9 pH Initial qO. 3 _ T. F (Z.T Final -7.-~~ 5->' Conducfivity-Intitfilal -6...1q 5 -_: 313 7,5) Z-Analyst Initials LGO FP4( 05,

.S fý.

QA Review Initials L FO L) k.-5: L-o 22.0% Outfall 001 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 7- - 5,1 15 Final I --37 S-. I D.O. Initial 7.-2._ Final U .M- '4 L.R (., *o._"_-_ pH Initial -,Oo c9 V.! I Final -7.7 j7-. -f -7.-q7. Conductivity-Intitial 3 C 4 Qq ________z Analyst Initials £7 5 QA Review Initials 0 _s0 L,5 so *._-,___-.__ .Notes: Page 1

.:*!JDaily cnemistry Daia iV -_.-4R. LoC'ation:L-Soddv'baiiy:Terrnessee Tempnlate No-. A ait nf1nru :Th-Iof. <--W I1 Y L ~ .,.~

-i~*..

c-.;:-. -"*Da 43.9% Outfall 001 0 1 2 3 4 5 6 7 Remarks Temperature-Initial zg. o _Y"_ Final 95~ 2H-.'9 2C.7J ?tO D.O. Initial "1

7. Z 7V Final

u.

7.2 D r pH Initial 1.41 7 -Tlq Final 7u.7.7T*o-")'-.

1. "5 Conductivity-Initial

-'N3 7 Q-7 o Analyst Initials S-_____"________ QA Review Initials V~r .-, i .o U iXo

-(o 72% Outfall 001 0

Day 72%Outfall001 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 0 1..So Final 4 + o on D.O. Initial 7 7

,,1 7.*2 Final U ACCLA r'-(

A pH Initial '7, 7q "t.* "t, Final 1r-_ 3, d'7,_iT-. Conductivity-Initial .)3', ,5 Zg'... = - 23_ Analyst Initials sD f -t ( o .\\ ¢-____.______ QA Review Initials L~v ) USE USO Zo L... Day 100% Outfall 001 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 6 4 0 D'-,0 Q_. Final i 9 -. 7 5

7-H. D;- 1. 3-.

7" D.O. Initial 7

7. -714 Final

.-7 -7. L, &A " I pH Initial

7. L, 7.-/ Z-Final7H RE--177 Alkalinity

( Hardness Conductivity-Initial 1q1 \\5 20.1 Chlorine go.o.

c. '

0.01 Analyst Initials 0 vc S o QA Review Initials Ls.Io L.2 SO k0. k.O L %S s )w Sample Date fablrt Wiz I o,-V1)12-121 jH ti,* 27-ji l Notes: Page 2

l h t.- thea "M -7. Chronic .'Daily Chemistry Data - Fathead Minnow 7-day Chronic Client: TVA - SeQuoyah Nuclear Plant Location: Soddy Daisy. Tennessee Tmplate No.: Analysts: vc* L )z-5 Dates/times: .i, a, Ii't/ IiDh' I3 A ge of larvae: i0-. Intake-100% Temrnerature-Initial Final D.O. Initial Final pH Initial Final Alkalinity Hardness Conductivity-Initial 0 1 2 Da 3 4 5 6 7 -'*2*" 25., 3 4.-7 IZ5. Z 2'. (C 5 1.4' 7i.1* -f' IW.CQ '.C "7.- I i~t-7 P I A..-, " 7 1 t -- lLi C Chlorine o.t1! Analyst Initials 11-So QA Review Initials Sp0 Sample Date rIio Notes o.c* [(-I-Ao--1(O tz(t"2 - ij. Cl -1 I I Remarks I. i It Page 3 2S, 1 26,( t j14 - "*qm ,j

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Sequoyah Nuclear Plant Biomonitoring November 27-December 1, 2001 Appendix F Void Tests Chain of Custody Records and Toxicity Bench Sheets

D=WIUJUNI I U1NN1 CHAIN OF CUSTODY RECORD Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: TN0026450 Collected By: WANDA ALLEN Field Identification / Sample Description 1413 Topside Road, Louisville, TN 37777 Phone: Fax: Collection Date/lime (If grab, list date/lime under "End") 865-970-0003 865-970-0004 I bContainer Number & Volume Flow (MGD (specify): V'. 4,. 1.,ý-'At,, Instructions: Clents should fill in all areas except those in the "Laboratory Use" block. Biomoniloring samples are preserved oring ring them at 4=C and shipping them in le.,'iThehbld time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will reoeive the samples winh ampletime l testing within that time frame. Samples shipped overnight on Friday via FedEx or UPSmust bemarkedforSaturdaydelivery ortheywilnotarveuntilthfollowing ondayij*l VA COC Page -- 1... of glient: TVA UPS Bus Client S & ME, Inc. . f.

BIOMONITORING CHAIN OF CUSTODY RECORD Piini I " M1ji~j Clint: M Project Name: SEQUOYAH NUCLEAR PLANT P.O. Number: PO BOX 2000 Facility Sampled: SEQUOYAH NUCLEAR PLANT NPDES Number: TNO026450 Collected By: WANDA ALLEN S & ME, Inc. 1413 Topside Road, Louisville, TN 37777 Phone: Fax: 865-970-0003 865-970-0004 ed UPS Bus Client Other (specify): Collection Date/Time Container Rain Event? Field Identification Grab/ Ship. (If grab, list date/time under Number & Flow Sample Description Comp. Temp. "End") Volume (MGD (Mark as Appropriate) I I(0c) Collected Start End Yes If Yes, No Trace Inches S-

==- SQN-DIS-101-TOX comp 4*C 11/27/01 11/28/01 (1) 2.5 SQN-INT-TOX comp 40C 11/27/01 11/28/01 (1)2.5 GAL / ~~~~~~~~~ UUIr iL4~'.~LIUU0V 1-i hf in 1rom Lo ODoIJfwnl______________ Relinquished By (Signature): Date/Time Received By ( a )tr S(S~~ignature): Date/Time :,,,, MY, SIln w Pr.1 .-4,* a fill I I. a, Ii a e excep t inose In m e Laboratory Use" block. Biom onitoring sam ples are preserved by storing them at 4*C and shipping them in ioq,.ThE me for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample tlmto-in )sling within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following MOE ~-.- I A COC A S............. . m = S.... t, i

BIOMONITORING CHAIN OF CUSTObY RECORD Page 1 of 1 dient: IVA UPS Bus Client Project Name: SEQUOYAH NUCLEAR PLANT S & ME, Inc. Other (specify): P.O. Number: -P. BX 00 1413 Topside Road, Louisville, TN 37777 Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone: 865-970-0003 NPDES Number: TNO026450 Fax: 865-970-0004 Collected By: WANDA ALLEN Collection Date/Time Container Rain Event? Field Identification I Grab/ Ship. (If grab, list date/time under Number & Flow Sample Description Comp. Temp. "End") Volume (MGD (Mark as Appropriate) (°C) Collected Start End Yes If Yes, No Trace I Inches SQN-DIS-lOl-TOX comp 40C /29/0 /0 1/2 9/0 11t/30/01 51 SQN-INT-TOX COMP 40C 11/29/01 11/30/01 (1)2.5 _,_ oC, __,,GAL Slq s e y (:at ime Csto d y -T o nD o R e l i n q u i s h e d B y ( S i geR e c e iv e d B y ( S ig n a t u r e ) : D a te.T.m e InSjamplten_ _t -Filln11Top Down... R eli q ui h ed B y Sig n tur ): ate ri m eR e c iv e B y (Sig atu e):D at [Tl ln dni~~n a ~ 1 nt L~ %i~I fil . 11 -- -- -- L vi a a ~ ~ u ~ up i os n e La or to y se b oc. Bi mo it ri g a m le a e re e ve b s or ng th m t C nd s ip in t e m In ic. Th el TVA COC reas

u, ex cept those in Ina -Laboratory Use" block.' Biomonitoring samples are preserved by storing them at 4 0C and shipping them In Ice.,, T,he hold time for each sample Is 36 hours from the time of collection. Therefore, please collectrand ship in such a way that the laboratory will receive the samples with ample time to.Wiltlate testing wthin that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

.'c.N* T FATHEAD MINNOW TEST INFORMATION Test Name: TVA-Sequovah Nuclear Plant Test Conducted Frorm: III 2.-oI (Day 0) To 12.t1 rý 0I/o (Day 7) Sites/Concentrations: .)

4.

2 ,9 /" ]o-4

2.

p0, 4r*9

5.

Stock (if applicable): Control Water Type (/): Moderately Hard Water + Trace Minerals Other (describe): _-1 Dilution Water Type (,/): Moderately Hard Water + Trace Minerals Other (describe):___ Source of Test Larvae (V): S&ME LO+-# oI - zz, Other (describe):_ Date Larvae Hatched: ii L t Hatch Time: 41.* Initials: _ }_'_ 11 r1'.- 1o' Record of Minor Test Non-Conformity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed By-(Reviewer Initials) on ._(Date) REV.01, JANUARY00 (fathead minnow test info)

4 1

-Client: TVA/Seauoyah Nuclear Plant -Any s... Clint:TVNeguyahNucearPlat -Analysts: Vq_.. b** rPs Location: Soddy DaisyQ Tennessee Dates: CUMULATIVE MORTALITY OF P. PROMELLA IN CHRONIC TEST REPLICATE A P r Mod. 23 i Hard 4 0 0 5 6 7 L'" 2 .6 3 10.98% 4 Outfall 5 6 7 " 1 0 C> ) 220% Y,,_) 4 Outfall 5 6 7 4-3 43.9% 4 O Outfall 5 6 7 2 '6 3 72% 4 Outfall 5 6 7 DAY 100% 7> 4 I Outfall 5 6 7 .2 2 100% 3 Intake .5 4 I C. 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 6 -7 REPLICATE DAY CONC. Initials NO.

Location: equ.:..-.. Test Type: Fathead Minbw 7-day Chromc S-:;Age of Larvae: *2 Control: Mod. Hard 0 1 2 3 4 5 6 7 Remarks Temperature-Initial Final 24 ,6 2 L 6 -q.-i D.O. Initial 7 pH inImntial V-W-- Final 12,1 7 q 7 Alkalinity I* e G*, tA Hardness P::Lgo Conductivity i'* 2.. Initials iý E ) D416_ QA Initials 0 L;O Day Outfall 101 - 10.98% 0 1 2 3 4 5 6 7 Remarks Temperature-Initial %,3 2Mt.4 It) Final 14 1 5 24

,, -7 D.O.

Initial -.- i 1 ~~Final pH Initial J., i-g5 - q, Final 7 ')A79 -7. Conductivity / 1 o 2____I initials %A, QA Initials Uto L5.0 "Oo _.-0 Da Outfall 101 - 22% 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 1n1A

1 Final D.O.

Initial Y 7 - Final c pH Initial ri, I 9 T~(____ Final Conductivity 01~ ?.A AI Initials 4 &)111

A&r QA Initials Us9

-S D I -SO I-SO nv-wv4CezA,'Ii *Ir-2Xg W% \\ocY-A.I do-ý,)4

en~U.TVA Locato:Sd d& a~Ji. Test Type:Ftha 10e/tms Outfalll 1 1-43.9% 0 1 12 Da4 6 7 Remarks Temperature-Initial 4.3 14,. 1~/ Zq Final )McIbzl.2 S6 D.O. Initial J.* 7. 7 Final

6. 7 u :.2 pH Initial

0) 911)

-Q I Final -7i (0_7_ __-7 Conductivity Oj 2607e Initials QAlInitials j11 Outfall 101 -72% 01 1a eak Temperature-Initial p

~

Final (p D.C. Initial 113 M-1 7.Z _L77 Final

6.

pH Initial 73-Final77T Conductivity ).j3 DL) 21.-5 Initials ~~ip A .QA Initials SnLS O Le.0 LEO Da~- Outfall 101 - 100% 0 1 2 3 4 5 6 7 Remarks Temperature-Initial 1 )q D.C. Initial 7(_ _H initial 1.13 41 TA_________Z Hardness70fD Conductivity I4O 19'2-4L ~ Chlorine K_- Initials V QA nitials LSO iff r ýý6%Y6 clot 4

W CýYT64d bt, PgPA,ý,oý 4 -ý

CE-RODAPffNA 'TEST INFORMATION SHEET Test Name: TVA --Sequovah Nuclear Plant TestConductedFro= 1112:1 (Dayo)To 1.log. )o] (-Day7) Sites/Concentrations:

i.

*---]4 L"*]

.- etmK

.i

2.

in,

5.

"7-I. Stock (if applicable):

6.

Control Water Type (v"): 20% Dilute Mineral Water + Trace Minerals Other (describe): Dilution Water Type (/): 20% Dilute Mineral Water + Trace Minerals Other (describe):___ Source of Test Organisms: S&ME brood board nos. i L12:, Age of Test Organisms: Isolated neonates for test onjIIa Dt (date) from 093CD to / *6_)O (time) Initials \\)Gi. Record of Minor Test Non-Conformity Date: Description of Non-Conformity: Initials: Date: Description of Non-Conformity: Initials: Test Log sheets QA Reviewed By: __(Reviewer Initials) on (Date) REV.01, January 00 (ceriodaphnia test info sheet)

Ceriodaphnia Daily Test Information Logsheet rest Name: TVA-Seguoyah Nuclear Plant Test Dates: 1 1/27/01-12/04/01 information i-speIauLi iin r-luluIon (cup -25+/-1°C) Date and Incubator Temp. (°C) Therm.

Fed 100 Initials No.

YCT Day_0____ a.m. p.m. Day 0'S 2-Day 14.6 Da,2 9, -C D 1 3c' i_ I 1 1 Day 5 Day 6 Day 7 9 I i-l Feeding Information uL YCTDate Fed lOOuL Selenastrum 1-7)' Selanastrum Date L4/* 0 W/Lvh, est miiua on Change, or Terminati Start Time Er 13/,12 I 13;o, /6 water Test ion Control Water Carboy No. and Additional comments nd Time Carboy # Date Prep. 1-33.1 _L # (( 'v/ j 2,$. 1 254[

~%~AV~~5JIL~iZJDUUU~~uviaiau

WUjpeprouucnon Client Dt he LU.ocato SdyDiyTeeseTest Start-Date/Time: Ij

~ j Dates Samp les Collected:, 11 1, I~z Test Stop-Date/Time: tttoqlicI 1 12 13 14 15 6 7 T~~~ 8 1 11-Ito-8 I 9 110 p 1 Number of Younz

of Live

.T' 1* 1 1 1 eniicate

f Number of OrignalYoung per
oft Adlt dults

't CJ) 7 1 1 I-T_ i_ LU LCL M 'k I I_.- III____III .1 I_____I_ Renli't 7 9 I 10 Number of VAII1,

of Live A A..1÷+.

7 1 1--

ot Number of Orignal Young per I2_

0 k0 10 3 __C C) 5 ( I IUW. I __________ I __________ I Conc. 10.98% Outfall Conc. 22.0% Outfall. 4 licate enlicntp. I -, I I Irdt.paý F;ýL2 I I

of Original A

2 3 4 5 6

.*:*.*1** awData Sllh" ~ .A

'Client: Tennessee Valley Authority-S "..

A...y h h-Location: Soddy Daisy Ternesee Test StartDate/rime* Dates Samples Collected: ! g Test toDate e At 27-b II I

of Live
of Number of Original Young per A

i y.l,-- **, g!llo AA.1... Adl = 1~ d '-.I 3r-> I I IT I 1 Init. Da

3.

5 6 7 Total Re ate

of Number of ea 14 6 89 Number of
of Live Original Young per 2

3 1 5 8 9110 Yo Adults Adults Adult 0 Jf._ 2 S o Replicate

of Number of Number of
of Live Original Young per 5

6 7 8 9 10 Young Adults Adults Adult C) (0 4

4.

r 1-f 4-3 r i 4 ' r- i 4 . 4 If~tFF1 J I-j i -J Conc. 43.9% Number of ".a Conc. 100% Outfall \\,"3 F"" "--- ---.J Io \\ t"b 1 I* "i "--10

_*0 t

Revliate 1 2 3 4 5_ 6 7 18 19 _ nt.Da) 0 2__ S4-3" 5 4 C ¢", /% Ouutall 5 6 -7 iTotal t'*_ _.*-*_ 1"/ "t Conc. I~nit. Da) 72% 4 Outfall 5 7 I Total O 4 "3

R 'Data Sheet" -~ Client: Tennessee Valley Auor.ity-s C.S Location: Soddy Da'syr Tennessee Test Start-Date/ime: 'L-t7/JA.Q Dates Samples Collected: A I -t)z Test Stop-Date/Time: 1-zjoq oU / 2 3 4 5 6 7_Ite--T10 Number of

of Liw.

rgia Ioc 2ni 3 7 8 9I Young Adults Adults Io c Dn. Dao -t Intake 5 6 7 i l

Total 4

Location -SoddyDaisvTND tfes Template No.: J gf~iats'<&~ Test Type: Ceriodaphnia 7-day Chronic 7-. Control: 20% DMW 0 1 2 3 4 5 6 7 Remarks Ter A a -Initial 9, 2 1 o ;Ls, ji q 11, D..IiilFinal 2 Y, 3 Z'tO Yz 0 Day Outfall 101 -10.98% 0 1 2 4 5 6 7 Remarks Temperature-Initial

q.Z *q_?ZZp,.**

Final 17 6b,.o 6,74 0 pH Initial FinalT Conductiity PI-7/ 70 t-75 I I~c Initials ,/*j QA Initials "r) Lso Jqbc* i Da Outfall 101 -22% 18 0 1 __1 2 3 4 5 6 7 Remarks Temperature-Initial q.34 2 C_ D.O. Initial~ia T

- - *-)'

70;*",7 pH Intial I,-*-, IL' Final 1 -,2 S, 2-1 'Conductivity %2.*L ý7 t* 71 QA Initials -Y4f~rnmICa<5

-{--S+ecj ALe 1 Ci cJK -

4 "7

~ll'A ll-ie u v h u l~ I~*- A na*st 1:. Location: So-ddy=aI..*L DD t':m s "-" "7, '1 1 1--, Teml No.: Age of Neonates VI 2)1 Test Type: Ceriodaphnia 7-day Chronic Outfal__l 10 1 - 43.9% 0 1 Final D.H. Initial 72 Final Conductivity 1 VI Intials QA Initials cs. Lao Outfall 101 - 72% 0 1 Temtperature-Initial Z Final iLt-Z, IS_ I __I____ NU Lk 25.3 7,1 _47_ Ia LILsI, I 2 Day 7 1 Rem arks f C =L_. 9M4 f ' pH Initial T. T i, I* *7 Conductivity i Initials

m QA Initials fgo L.- Q -&O Outfall 101 - 100%

0 1 2 3 4 5 6 7 Remarks Ternperature-initial '$o 2 ~q,,) 2i,- Fia 2S.,0 N Final i" PH-Initial

,.2

-m Co inity "m Hardness-7. a, Conductivity t, ,*{

!]

Chlorine ,./ .r" "InA Tn;* *... '-I I I 4 LX) A;7ý I L.-S D ug USP v%_IL-7 litz.7 WTI 4#121 D Výzo I i-S-0 IL-"o n I Y. 3 4 5 1 6

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ML020430548

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ML020430548

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Dear Ms. Haskin:

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