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Discharge Monitoring Report for July 2005
	ML052270384
Person / Time
Site:	Sequoyah
Issue date:	08/11/2005
From:	Howard S; Tennessee Valley Authority
To:	Hannah C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
	Download: ML052270384 (110)
	v • d • e

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 August 11,2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6t" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JULY 2005 Enclosed is the July 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue A Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 I'Ed on reyldpa

PERMITTEE NAME/ADDRESS (Include Fadity Name/locaton if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. tame TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 Address P.O. BOX 2000 (SUBR 01) _ _ _ _1tfTEjOFFICE aN-2T - - - - - - - - - I TN0026450 101 G lF - FINAL ____A(LD~YO _ DAISY_ TN 37384 I PERMIT NUMBER IDISCHARGE NUMBER DIFFUSER DISCHARGE FaWy_ TVA ,SEQUOYAH NUCLEAR PLANT Location _AMILTON COUNTY __________ Il fi MONITORING PERIOD

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I YEAR JMO IDAY- I I YEAR I MO DAY FromI 05 1 07 1 01 I NO DISCHARGE a *-- ATTN: Stephanie A. Howard Tol 05 1 07 131 1 NOTE: Read Instructions before completina this fomn. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE _ _ _ _ _ ___ _ _ _ _ _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE ******* *** ***** 30.0 04 0 31 131 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 EMT ***I*5V e~:~ - -eee DGC SEE" 'CK REQ~ INSTREAM MONITRN ROIEEtNT'f'~.-..~_____~- PERMIT-TEMPERATURE, WATER DEG. SAMPLE *** ***** 42.8 04 0 31/31 RCORDR CENTIGRADE MEASUREMENT . 00010 1 0 0 .D PERMIT, DEG.C.-` `',e**SEE.**.; PMEQUIREMENT * * .. 7E0 7 C. O SE CK REB EFFLUENT GROSSVALUE . . __ AILY MXU -- PERMI MEASUREMENT 7.0 7.2 12 0 8/31 GRAB 00400 1 0o o EMT.~'~1'****~Z .. _ ~ CC*CCVf%" .-090-&S MGD .. WEEKLY GRB EFFLUENT GROSS VALUE MEASUREMENT I MAX IM U M.. EFFLUENTGROSSEVLU 50050 1 0 0 - MREASUREMENIT

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NAMETITE PRINCIPAL EXECUTIVE OFFICER I CertifYlwdrpenaltyOf law thatNt~sdoa~ewrintandalettadviwtsWWereprepared undermfy TELEPHONE DATE

                                                    .tdrection or aupervfslonIn accoran w i~hasystem deslgnedtoassue tatqualiriedpersometI J. Randy Douet                        poerygater and evaluate the Infonrmation submhtted. Bassed on my Inqury of the person or              1        4      4          .&~              :Q Site residento Vice                                   manage the system, or those persons dilrectly responsible for gathering the                   Principal Environmental Engineer                 42       83670                 5    08       1 SiteVic Prsidntacoxate,                           and complete. I am a*wae that thee ame  signIficanit penalties for submitting false            SIGNATURE OF PRINCIPAL EXECUTIVEI TYPD RPINEDInformation, irduding Mhepossibility of fine an Imrsomn fo kr~n vIlto                                                                 OFFICER OR AUTHORIZED AGENT                     AREA INUMBER               *YEAR     MO      DAY TYEDO PITEI                                                                                                                                                                                           CODE I_________________________J______________

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceaeliaefachmrrents here) No Closed mode operation. CCW data for July 2005 Is attached. Veliger monitoring Information Is attached. CrA ro0 J3ZU-1 tKtV 3XS-) f-MVIOUS MITI= May De usea Page I of 2

CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 07/01/2005 0720 <0.5 mg/L 07/06/2005 2317 JBR EPA 8015B CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 07/01/2005 0715 <0.5 mg/L 07/06/2005 2239 JBR EPA8015B

Sequoyah Inplant Sampling Mean# of Water Mean#of Water SAMPLE Sample Date ZM~mt % Settlers Temp. Sample Date Asiatic Temp. LOCATION SUB LOCATION COLLECTED BY ZflI(,C) Clamslm CC) 04101/2005 0 0 14 04/01/2005 29 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/06/2005 0 0 13 04/06/2005 81 13 INPLANT Inplant Raw Water QUANT Dick Adcock 04/08/2005 0 0 14 04/08/2005 54 14 INPLANT Inplant Raw Water QUANT Dick Adcock 04/13/2005 0 0 16 04/13/2005 106 16 INPLANT Inplant Raw Water QUANT Dick Adcock 04/15/2005 0 0 16 04/15/2005 0 16 INPLANT Inplant Raw Water QUANT Dick Adcock 06/23/2005 432 50 25 06/23/2005 154 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/24/2005 162 80 25 06/24/2005 30 25 INPLANT Inplant Raw Water QUANT Dick Adcock 06/29/2005 290 80 26 06/29/2005 168 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/01/2005 318 100 27 07/01/2005 127 27 INPLANT Inplant Raw Water QUANT Dick Adcock 07/06/2005 0 0 26 07/0612005 677 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/08/2005 122 29 26 07/08/2005 1132 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/13/2005 51 33 26 07/13/2005 998 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/15/2005 0 0 26 07/15/2005 186 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/20/2005 38 50 26 07/20/2005 303 26 INPLANT Inplant Raw Water QUANT Dick Adcock 07/22/2005 0 0 27 07/22/2005 100 27 INPLANT Inplant Raw Water QUANT Dick Adcock 07/27/2005 143 100 27 07/27/2005 341 27 INPLANT Inplant Raw Water QUANT Dick Adcock 07/28/2005 488 100 27 07/28/2005 627 27 INPLANT Inplant Raw Water QUANT Dick Adcock 07/29/2005 90 20 27 07/2912005 663 27 INPLANT Inplant Raw Water QUANT Dick Adcock 08/03/2005 0 0 27 08/03/2005 500 27 INPLANT Inplant Raw Water QUANT Dick Adcock

quoyah Forebay Sampling MaofWater Mean# of Water NOTES: % SB SML mple Date Mean#of % Settlers Temp. Sample Date Asiatic Temp. Gravid LOCATION SUB TYPE COLLECTED BY zMm 3 3 Asiatic LOCATION TYPE C)Casr C) Clam 116/2005 0 0 7.9 02/16/2005 0 7.9 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 123/2005 0 0 8 02/23/2005 0 8 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 128/2005 0 0 7 02/28/2005 54 7 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe

;/07/2005     0          0        7   03/07/2005     82        7       0    Outside Plant  Forebay Quant             S Jones-Brier, Chris Coe
;/1 5/2005    0          0       10   03/15/2005     122       10      0    Outside Plant  Forebay Quant             S Jones-Brier, Chris Coe 1/21/2005     0          0       10   03/21/2005     105       10      0    Outside Plant  Forebay Quant             S Jones-Brier, Chris Coe 1/29/2005     0          0       12   03/29/2005     101       12      0    Outside Plant  Forebay Quant        S Jones-Brier, Chris Coe,JRigsby 1/05/2005     0          0       13   04/05/2005    525        13      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby 1107/2005     0          0       14   04/07/2005    252        14      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby i/04/2005     0          0       17   05/04/2005      0        17      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby
;/09/2005     0          0       18   05/09/2005     632       18    60%    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby
;/12/2005     0          0       18   05/12/2005    549        18      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby i/17/2005     43         50       23   05/17/2005     106       23      0    Outside Plant  Forebay Quant             J Rigsby, M MacCarroll il19/2005      31        50       21   05/19/2005    299       21     33%    Outside Plant  Forebay Quant       S Jones-Brier, J Rigsby, MacCarroll 3/23/2005    579        50       20   05/23/2005     22        20     0%    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby 5/26/2005    242        50       20   05/26/2005     61        20      0    Outside Plant  Forebay Quant       S Jones-Brier, J Rigsby, MacCarroll 3/30/2005    1530        26       22   05/30/2005    1831       22       66% Outside Plant  Forebay Quant             S Jones-Brier, J Rigsby 3102/2005    1344        30       22   06/0212005    1989       22      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby 3/07/2005     240        40       23   06/07/2005    613        23      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby 3/09/2005     192        40       24   06/09/2005    425        24      0    Outside Plant  Forebay Quant   S Jones-Brier, J Rigsby, MacCarroll, Shaffer 3/13/2005     236        42       24   06/13/2005    928        24      0    Outside Plant  Forebay Quant             J Rigsby, M MacCarroll 3/15/2005     78         33      24.5  06/15/2005   2491      24.5      0    Outside Plant  Forebay Quant              S Jones-Brier, J Rigsby 3/23/2005      0          0       24   06/23/2005     171      24       0    Outside Plant  Forebay Quant                     W. Allen i/24/2005      0          0       24   06/24/2005    784       24       0    Outside Plant  Forebay Quant                     W. Allen 3/27/2005      0          0       24   06/27/2005    305       24       0    Outside Plant  Forebay Quant                     W. Allen D/30/2005      0          0       24   06/30/2005      0        24      0    Outside Plant  Forebay Quant                     W. Allen 7/25/2005     24        100       27   07/25/2005    1205      27       0    Outside Plant  Forebay Quant             S Jones-Brier, J Rigsby 7/28/2005      0          0       27   07/28/2005      0       27       0    Outside Plant  Forebay Quant S Jones-Brier, J Rigsby, M Wallus, G Shaffer, M l 8/01/2005     28        100       28   08/01/2005    1505      28       0    Outside Plant  Forebay Quant             S Jones-Brier, J Rigsby

PERMITTEE NAMEIADDRESS (Include Facility Namel.ocation ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Na TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P BOX 2000 ___L[NTEIOOMIE SIO2) __

                                    - -     -   -    -   -    -_                                      I         TN0025                                             101  G          F - FINAL SODDY-DAISY         TN 37384 _______                                                        I       PERMIT NUMBER                        DISCHARGE NUMBE                 DIFFUSER DISCHARGE Fdjy. VA-SEQUOYAH NUCLEAR LNT____

Location HAMILTON COUNTY __________ I , ftnNmTrnRlimr. PFRIODf IylV,l,wV,llrV F-lRlVy I EFFLUENT E ATTN: Stephanie A. Howard m05 1 07 l 01T o 05 l07 1 31 NO DISCHARGE m NOTE: Read Instructions before completing this form. NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this dowrtent and anlattadyents were prepared under my Al1t TELEPHONE DATE J. Randy Douet drectionorsupervisioninaccodancewithasystemdesignedtoasse thatfiualiaedpersonnel (j( 9A[.di properly ather and evaluate the Information submitted. Bssed on rmyInquiry of the person or .- t v. persons who manage the system, or those persons directly responsible forgathering the Principal Environmental Engineer Inforrmation, the Inrormeton submitted Is. to Om best of my iedge and belief, true, OF423 843P700 05 08 10 Ste Vice President crate, and complete. Iem ewamr that there we significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTiVE TYPEDORPRINTED information. Inckiding tha possibility ofine and priornent for knowing violtions. OFFICER OR AUTHiORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ __ CODE COMMENTS AND EXPLANATION OF ANY ViOLATIONS (Reference aff attachments here) The following Injections occured: 1. PCL-222 Copolymer (max. calc. conc. was 0.019mgL-Iimit 0.2mg/L) 2. PCL-222 Phosphate (max. cale. conc. was 0.054mg/L-limit 0.2mgQL) 3. Blodetergent 73551 (max. calc. conc. was 0.014mgQl-lmit 2.0mgQL) 4. H-150M (max calc. conc.was 0.0369mg/L-limit 0.050mgQL) 5. H-150M (low detection level analytical method was <0.020mg/L-limit 0.050mglL) EPA EPA Form 3320-1 (EV orm33201199) Pre~ousediion maybe sed ageof (REV W99) Previousedillons may be used Page 2 oF 2

PERMITTEE NAME/ADDRESS (Include Facilit NameltocatIon IfDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For rmApproved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ___I qNTEROFC M SB-,ZN . . . . . ----.. I TN0026450 101 T F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER [ ! ISARGE NUMBER BIOMONITORING FOR OUTFALL 101 Fadly VA.SEQUOYAHNNCLEARA PLNT _ Location HAMILTON COUNTY IMONITORING PERIOD I EFFLUENT IYEAR I -MO I DAY lI YEAR I MO I --DAY Il-* rknE.g-L A r *** ATTN: Stephanie A. Howard Froml 05 1 07 1 01 I Tolo5 0 07 1 31 1 NOTE: Read InUsructo Lo o NOTE: Read Instructions before comptetinq^ this fhmm NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Csrtfy under penalty of law thal ths document and eti atladvnentas were praepard under my A direction or supervison hI accordance with a system designed to ass"re that quaruied personnel Q ,rUiIte. (,.4

Y TELEPHONE DATE persons who manaes the system, or those persons drwecty responsible for gathering the Principal Environmental Engineer Sit Vi PeIdet ifomabon.the Itlormatlon submitted Is. the best of my knoledge andbelief. true. ______423 843_6700 05 08 1_0 e Me resen orate, and comptete. am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED IINformation, hIcuding the posshility of fine and hIprisonment for knowhg violations.

________________________________________________CODE COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference all attachments here) OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY Toxicity was sampled July 17-22, 2005. Report Is attached. r-rvru earn mayoe_,_ usei Pag 1fof 1^snX.__:__. tPA Forr^ 33Z2t-7 (RtV;3f99) 1-mmfius eamions maybe used Page 1 of I

August 9, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, JULY, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected July 17-22, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. In addition to the routine compliance test, fathead minnows were also tested in Outfall 1.01 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality was not demonstrated in minnows exposed to routine compliance samples. Reduction in growth was statistically significant in UV-treated intake samples. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services- West CTR 2L-M Attachment cc (Attachment): Files, R&TA, CTR IB-M SQN July 2005M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: August 9. 2005

1. Facility / Discharger: Seguovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3,266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQ10: 2.992.4

8. Outfall Tested: 101

9. Dates Sampled: July 17-22. 2005

10. Average Flow on Days Sampled (MGD): 1566. 1570. 1569

11. Pertinent Site Conditions: H-130M molluscicide was injected from July 17-22, 2005.

The dates and times for the H-130M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period. l Injection Location l Date/Start Time (ET) I Date/Ending Time (ET) Essential Raw Cooling Water (ERCW) Train A 07/18/05 ( 0930 07/20/05 @,1035

12. Test Dates: July 19-26. 2005

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphniadubia)

15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9, 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0, 100.0 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC, 5 >43.9%

17. Test Results: Outfall 101: Pimephalespromelas:IC25 > 100%

Ceriodaphniadubia: ICQ5 > 100% UV treated Outfall 101: Pimephalespromelas:IC,5 > 100%

18. Facility

Contact:

Stephanie Howard Phone#: (423)843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone 4: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected July 17-22, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were >

100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Reduction in growth was statistically significant in UV treated intake samples. 2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (EST) Time (EST) Temp. TRC* Time (EST) ID Collected Received (mL) Used By 101 07/17/05 0757 to 07/18/05 1342 2.6, 2.5 <0.10 07/19/05 1313 07/1 8/05 0657 ____ 07/20/05 1252 Intake 07/18/05 0636 07/18/05 1342 2.9 <0.10 07/201/05 1252 101 07/20/05 1320 2.3, 2 .0 ' <0.10 07/21/05 1305 _____ 07/20/05 0714 07/22/05 1245 Intake 07/19/05 0756 to 07/20/05 1320 1.7 <0.10 07/21/05 1305 ______ 07/20/05 0656 ____ 07/22/05 1245 07/2105 077 to07/23/05 1305 101 07/22/05 0637 07/22/05 1315 2.3, 27t <0.10 07/24/05 1242 07/2/05 63707/25/05 1229 07/21/05 0722 to 07/23/05 1305 Intake 07/22/05 1315 2.8 <0.10 07/24/05 1242 07/22/05 0622072/519

 *TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 1 1.0 0C) in a warm water bath.

Aliguots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart< UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes. 3

Pimephalespromelas Ceriodaphniadubia Test Organisms:

1. Source: Aquatic BioSystems, Inc. In-house Cultures

2. Age: 22.25-23.75 hours old <24-hours old Test Method Summary:

1. Test Conditions: Static. Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4 10

5. Organisms per Replicate: 10 I

6. Test Initiation: (Date/Time)

Outfall 101 07/19/05 - 1313 ET 07/19/05 - 1200 ET UV Treated Outfall 101 07/19/05 - 1300 ET

7. Test Termination: (Date/Time)

Outfall 101 07/26/05 - 1246 ET 07/26/05 - 1117 ET UV Treated Outfall 101 07/26/05 - 1233 ET

8. Test Temperature: Outfall 101: Mean = 24.7C Mean = 25.0C (24.1-25.30 C) (24.5-25.5 0 C)

Test Temperature: UV-Treated Outfall 101: Mean = 24.70 C (24.1 -25.30 C)

9. Physical / Chemical Measurements: AlkalinitL, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tideoool Scientific Software. McKinnevville, CA).

4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration) Conducted July 19-26, 2005 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%'Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 98 98 93 93 Test Solutions Mean Dry Weight (mg) (% Effluent) 12(replicate number)_____ (%Effluent)_1l 2 3 4 l Mean Control 0.696 0.716 0.613 0.698 0.681 10.98% 0.701 0.729 0.679 0.746 0.714 22.0% 0.675 0.698 0.703 0.642 0.680 43.9% 0.678 0.655 0.665 0.625 0.656 72.0% 0.713 0.792 0.726 0.753 0.746 100.0% 0.751 0.653 0.677 0.699 0.695 Intake 0.704 0.660 0.514 0.715 0.648 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

    *TlUa = I00/LC5o: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicitv Test.

(Genus species) (Type / Duration) Conducted July 19-26. 2005 using effluent from Outfall 101. Percent Surviving Test (time interval use - days) Solutions 1 2 3 4 5 6 7 (% Effluent) I Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100

           *72.0%        100        100       100      100         100    100    100 100.0%        100        100       100      100    . 100    100    100 Test Solutions                    Reproduction (#young/female/7 days)

(%Effluent) - Data (replicate number) _______ 1 l2 3 4 5 6 7 8 9 10 Mean Control 30 27 28 31 28 28 28 30 26 28 28.4 10.98% 32 29 27 29 32 30 33 27 33 32 30.4 22.0% 32 32 35 31 31 28 29 31 30 32 31.1 43.9% 34 35 33 35 32 34 29 35 36 32 33.5 72.0% 35 33 32 37 31 33 33 31 36 30 33.1 100.0% 33 34 34 32 33 31 34 34 37 34 33.6 IC 25 Value: > 100% Calculated TU Estimates: <1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

     *TUa = I00/LC 50 : TUc= 100/IC25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration) Conducted July 19-26. 2005 using water from Intake Percent Surviving Test (time interval used - days) Solutions I 2 3 4 67 (% Effluent) 1 2 3 4 5 6. 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (%Effluent) Data(replicate number) 1 3 4 5 6 7 8 9 10 Mean Control 31 31 30 29 29 29 29 28 31 27 29.4 Intake 30 29j32 .36 28 29 33 30 27 31 30.5 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

     *TUa = I00/LCso: TUc = 100/ IC 25 7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration) Conducted July 19-26. 2005 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 98 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 93 Test Solutions Mean Dry Weight (mg) (% Effluent) 1 2 plicate n Mean Control 0.712 0.741 0.761 0.828 0.761 10.98% 0.798 0.798 0.700 0.686 0.746 22.0% 0.700 0.677 0.656 0.780 0.703 43.9% 0.774 0.745 0.748 0.772 0.760 72.0% 0.792 0.788 0.732 0.751 0.766 100.0% 0.720 0.688 0.731 0.921 0.765 Intake 0.596 0.706 0.722 0.654 0.670 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

      *TUa = 100/LC 5 0: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25 ) Pimephalespromelas July 19-26, 2005 1332 7-days KCI 0.62 g/L Ceriodaphniadubia July 12-19, 2005 0845 7-days NaCl 1.06 8

PIIVSICALICIIFNMICAI,

SUMMARY

Water Chemistry Mean Values and Ranges for Pirnephalespr-omelas and Ceriodaphnfi dubia Tests, Sequoyah Nuclear Plant Effuent (SQN). Outrall 101, July 19-26, 2005. Test Sample ID Temperature (*C Dissolved Oxygen (mg/L) pit U.) Conductance Alkalinity llardness Total Residual _______Initial Final Initial Final Initial Final (jimhosfem) (mgIL CaCO1 ) (mglL CaCO 3 ) Chlorine (mgIL) Control 24.9 24.4 7.8 7.2 7.84 7.68 300 60 84 24.7 - 25.1 24.2 24.7 7.6- 8.1 6.9- 7.5 7.68 - 7.92 7.55 - 7.80 290 315 59 - 61 82 - 86-10.98% 24.9 24.4 7.6 7.1 7.85 7.66 278 -- _____ 24.7 - 25.1 24.2 - 24.6 7.4 - 7.8 6.7- 7.3 7.75 - 7.93 7.51 - 7.79 268 - 296 - - - - 24.9 24.4 7.6 7.0 7.83 7.63 258 -- 22.0% 7.90 7.46 7.74 236 274 24.7 25.2 24.2 - 24.7 7.4 -7.8 6.6 -7.4 7.73 - - - - - -________ 4 49% 25.0 24.4 7.6 7.1 7.79 7.63 229 - ioI 24.8 -25.2

                                   .__              24.1 -24.6     7.3    -   7.9 6.8     - 7.4 7.68    -   7.86 7.47    - 7.71  224      -241                -     -           -   --

7.% 25.0 24.4 7.7 7.1 7.74 7.60 187 - _____ 24.9 - 25.3 24.3 -24.7 7.4 - 8.0 6.8 - 7.5 7.63 - 7.80 7.38 - 7.71 183 - 197- - -- 100.0% 25.0 24.4 7.6 7.1 7.69 7.59 143 50 58 <0.10 _____ 24.9 - 25.3 24.1 - 24.6 7.3 - 7.9 6.6 - 7.3 7.59 -7.77 7.34 -7.70 139 - 1SO 50 - s0 56 - 62 <0.10 -<0.10 Intake 24.9 24.4 7.6 7.2 7.70 7.61 144 52 63 <0.10 24.7 - 25.2 1___________ 24.3 - 24.6 7.3 - 7.9 6.9- 7.5 7.60 - 7.80 7.39- 7.71 140 151 SI 52 56 - 70 <0.10 - <0.t0 Cnrl 24.8 25.0 7.8 7.6 7.84 7.85 300 60 84 - Cnrl 24.5 - 23.2 24.8 - 25.2 7.6 - 8.1 7.4 - 7.6 7.68 - 7.92 7.78 - 7.91 290 - 315 59 - 61 82 - 86 - 10.98% 24.9 24.9 7.6 7.5 7.85 7.85 278 -- 24.6 - 25.2 24.7 - 25.3 7.4 - 7.8 7.3- 7.8 7.75 - 7.93 7.77 - 7.91 268 - 296 - - -- 20% 24.9 24.9 7.6 . 7.7 7.83 7.84 258 - - 24.6 -25.1 24.7 -25.3 7.4 - 7.8 7.4 - 8.0 7.73 -7.90 7.76 - 7.90 236 274 - - - 25.0 24.9 7.6 7.7 7.79 7.82 229 - - 43.9%__ 24.6 - 25.3 24.7 -25.2 7.3 - 7.9 7.4 8.1 7.68 - 7.86 7.74 - 7.89 224 241 - - - -- 2.% 25.1 24.9 7.7 7.6 7.74 7.80 187 - _____24.7 - 25.3 24.8 - 25.3 7.4 - 8.0 7.4 - 8.l 7.63 -7.80 7.72 - 7.87 183 197 -- - 10.% 25.1 24.9 7.6 7.7 7.69 7.77 143 50 58 < 0.10 100.0%_ 24.8 - 25.5 24.7 - 25.3 7.3 - 7.9 7.5 - 8.1 7.59 - 7.77 7.67- 7.83 139 - ISO 50 50 56 - 62 <0.10 - <0.10 Intake 24.9 25.0 7.6 7.5 7.70 7.75 144 52 63 <0.l0 _______24.7 - 25.2 24.7- 25.3 7.3 - 7.9 7.4 - 7.6 7.60- 7.80 7.64 - 7.8i3 140 - 151 51 - 52 56 - 70 <0.10 - <0.10 Overall temperature CC) Average Minimumn Maximum Pirnephales prornelas 24.7 24.1 25.3 Ceriodeiphnia dlubia 25.0 24.5 25.5 9

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pitnephalespromelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, July 19-26, 2005. Test Sample ID Temperature CC) Dissolved Oxygen (mg/L) p1-1(S.U.) Conductance Initial Final Initial Final Initial Final (limhos/cm) Control 24.9 24.5 7.7 7.3 7.84 7.68 290 24.8 - 25.0 24.2 - 24.7 7.5 - 7.8 6.8 - 7.6 7.75 - 7.91 7.54 - 7.79 276 - 311 10.98% 24.9 24.3 7.6 7.1 7.84 7.65 278 1 24.8 - 25.1 24.1 - 24.6 7.5 - 7.8 6.7 - 7.6 7.74 - 7.91 7.53 - 7.77 271 - 290 22.0% 25.0 24.4 7.7 7.1 7.83 7.64 259 24.7 - 25.2 24.2 - 24.6 7.5 - 7.8 6.6 - 7.6 7.72 - 7.89 7.46 - 7.75 237 - 275 C 25.1 24.4 7.7 7.0 7.80 7.60 236 2 .0% 24.8 - 25.2 24.2 - 24.6 7.6 - 7.8 6.4 - 7.6 7.70 - 7.88 7.43 - 7.72 224 - 259 25.1 24.3 7.6 7.2 7.77 7.62 195 z 24.8 - 25.3 24.1 - 24.5 7.4 - 7.8 6.8 - 7.6 7.66 - 7.84 7.46 - 7.71 185 - 228 i 100.0% 25.2 24.3 7.7 7.1 7.71 7.57 149 24.9 - 25.3 24.1 - 24.7 7.5 - 7.9 6.7 - 7.6 7.57 - 7.80 7.41 - 7.69 139 - 185 Intake 25.0 24.3 7.7 7.2 7.72 7.60 142 24.7 - 25.2 24.1 - 24.6 7.6 - 7.8 6.9 - 7.5 7.62 - 7.82 7.43 - 7.76 139 - 150 Overall temperature C0C) Average Minimum Maximum Pimephales promelas 24.7 24.1 25.3 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected July 17-22, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Reduction in growth was statistically significant in UV treated intake samples. 11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimerphalespromelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphniadubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephalespromelas None

2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephalespromelas None

2. Cerioda hnia dubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360. 1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8. Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by EPA Method 330.5.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2 s values in g/L KCI or NaCi.

2. Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephalespromelas.

Sodium Chloride (NaC1 crystalline) for Ceriodaphniadubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPAI600/4-79/020 (March 1983).

4. Ouality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-I . Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 I 3

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005

                . Sodium~tS             .-PCL222T
                                        . - ,... Il t Hypochioritep
              .. mgfL t .                 Phosphat'.
              - .TRC Q . i                       Ž.. ,

4

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 5

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 I I 6

Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

                                                                        .DIOMONITORING CHAIN OF CUSTODY RECORD                                                           Page_1           of     3_

Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments. NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Wanda Allen Fax: 828-350-9368 ' cH Field Identification / Grab/Comp. Collection Datelune Container Flow Rain Event? Sample Description Number & MGD F T Rn Even ColcinDate/Timev~ Volume (Mark as Appropriate) Dat.i m Nc..... sj, tc...;b@ ImaNo ____________________ __________________ Collected TimDaea Yes If YMs No Tae-SQN-101-TOX Comp 7117/05-7/1 1 K. 2 a.eTra NA57l N v11 et,,/ Wad le a 71/05Express Sal C - I Courier,0/~oo 07/18/2005of Lz> Express Courier u""d07/18/2005 ETS 07/18/2005 _ __ _ _ _ __ _ _ _ _ c-Instnrctions: Clients should rill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday dclivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 2 of 3 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 ' Collected By: Wanda Allen Fax: 828-350-9368 Field Identification Grab/Comp C Container Flow t AmqY~"nlPI1 t Sample Description Number & (MoD) Rain Even Volume (Mark as Appropriate) D ___________ Collected - .~ Date Tim j, e I e, N re Am~Trnp' wS~ Pyv Tera ppa SQN-101-TOX Comp 7/19/05-7/20/05 2 (2.5gal) NA i. b0sa 08Yf g8t Wanda Allen 07120/2005 Express Courier 07120/2005 ExpressAln Exrs Core

                                         ,         , o 07/20t2005 072.20            1        >

xpreTS C_______ ES0/020 7-7Z 07/20/2005 I Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via Fedl~x or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

UlOMONITORING CHAIN OF CUSTODY RECORD Page_3 or 3 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street FedEx UPS Bus Client P.O. Number: N/A Ashville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: C NPDES Number: TN0026450 Phone: 828-350-9364 4 C'44 - 4 Collected By: Wanda Allen Fax: 828-350-9368 Field Identification / Grab/Comp CollectionDateTime Conter ( Flow l F < . Sample Description ColNumberDat ime(MOD)nerRalin Event? X oa~i e~~'~: Volume (Mark as Appropriate) _ _ Collected. . .... ......

                            .                    DaIf           l    T                   _            Yn        Yes,      No     Trace   *A8      g                nX                lcTtce SQN- 101-TOX              Comp         7/21/05-7/22/05                         2 (2.5gal)      NA                    -Z                                            1                   -             .i SQN-INT-TOX               Comp         7/21/05-7/22105                           (2.5 gal)     NA                                                                             _

Sample Custody - Fill In From Top Down Relinquished By (Signature): DateiTime Received By (Signature): Date/Time Wanda Allen 07/22/2005 Ex ss ou 07/22/2005 Express Courier j .26. 07/22/20053 8 ETS 07/22/2005 v Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testincr Solutions. Inc. Page I of 6 Environmental Testing Solutions. Inc. Page 1of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA County: Hamilton Facility: Sequovah Nuclear Plant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: 1'4i I Dilution re n information: .Comments: Dilution prep (%.) 10.98 22 43.9 72 100 Effuent volue (niL) 274.5 550 1097.5 1800 2500 Diluent volume (nL) 2225.5 1950 1402.5 700 0 Total volume (nL) 2500 2500 2500 2500 2500 [ Test organisminformation: Test information: Organism age: zz. ZS -M5 tb to J es L Randomizing template: Date and times organisms l LIZ-OS 1X_3C -i-o %tCO Incubator number. were born between: ___ Organism source: Q1eS BP n-cIt-OS Artemia lot number. v Transfer bowl information: pH Temperature = C Total drying time: 2-l.l o0J i&

                                                                                 .zIA-3            DatelTimein:         rZ4b        131O Average transfer volume:                                                                  Date/ Time out: 01.1             1. 1%

Oven temperature: Dailyfeeding and renewal information: Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used I _time termination time t__ 0 cri- o S _ _ _ C1 Ls -O ( 3" t. I 02-t0 d alc-'ZO-Oi AO rzS

                                             %mm                               2-.         (31iiIOS6 0               IR. 01 XO2-z 2      al zt-e S            So3c5     __  Mao  __                                            t 13-6          cscfl2o.O5 -       & A
    .3     Q1-22.-O          DROS       _S_           _                  2I4               avi34B-%           oisao .ok4 OL 4       CT1-24c0s          09m            15c1A                     1-3a5              0M-1-65            C0a Mr 22.O    0l-II 5     11 2'k-205       M:5-             l56 I                    I ZA 7             al"-i2        Ak S01i2.08 Soz gYi 6  ali-25-05              otsz             tASS             1          z 2             al        .XS A,    6s n a .6V4()a2_

6 £32 O65 +/-'~;W^ 12- z'tl 2i-+/-. _Q .¶>;- -A j Control information: Acceptance criteria Summary of test endpoints:

       % Mortality                                           Al            S           200/              7-day LC50         l       I Average weight per initial larvae:         O . 0

NOEC 1007.,

Average weight per surviving larvae: 0. LAI 0.25 mgfarvae LOEC l OC)' , ChV IC2 s 1 >1 0073

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimephalespromelas Date: 1i 4-0o Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I J K L 0 10 IC) 10 It tO 10t 1 tO 1I 0 10

10. 100 '_ 10 (I)D 10 /0

____ __ /0 IC)t) -to IC)/0/ 101ID/0)0O0 JO /00 JO to too/ lo 2 tO lO lO (0 tO lO to 10 ID 10 to /0 4 /11) J 16 10 } ))e MDZ)}

                                                                                                                           /0          l 1L 10ID        1b       1                 J       M10          10 l 10D                          10 6                   l      1010l)10                 /010               10 1(          10)           to      1I)
                                       ?1)    Io       10               _

IC to 10 10 I O* 1U A - Pan weight (mg) & - . - a Colorlde t~f ratin 16.2 1.- IS-IL I6 M4S 1q.8S6 1 .,5, 110.2% I 16.4 .,12_ l. *9 16 .8 lL ,31 B - Pan + Larvae weight (mg) Analyst: KT__ _a a3. a 3.4 at a1 . 1.93 I.)31 2 3 23.0O _ . 3.l1 .ai 3 Larvae weight (mg) - A - B w lb.Xs (A& 1 .o\ 1n I.z.1i5 .L1 ti .1S _ 7. o3 Z Weight per Initial number of larvae (mg) N O-

    - C I Initial number of larvae 40      'IO-                            A
                                                                                                            ,'14                          .\
                                                                                                                                         'a V7tr                    0'4              to                            o0 0s_      _~

O. a *a *a
a
a Average Percent weight per reduction InitIal from control a 1( 0.-%'A - LL Sol. 0.2 T0 number of (%)

larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r - decanted and returned, w = wounded. a Calculations and data reviewed: _ Comments:

                                                                                                                                                          *-= .      .   .k.OU        :
                                                                                                                                                                .   .      .;:s   .,e Environmental Testing Solutions, Inc.                                                                                                                 Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated                                            Date:           a-Iig-oC; Survival and Growth Data Day                                 43.9%                                    72%                                  100%

M_ _ N O P Q R S T U V I W X 0-1 iooi _ 0, 10 1t 10l0(b iO IO 1 10 /0 10 1 0 10 / 0 10 10 10 / 0 10 1D 10 c 1O IC 10 10 tO 10 lO /0 l I0D _1 II 1 0 I0 1) 10 I 10 10 ______ _ 10-0IQ10 10 1QI1Dli_/O 10 _0 0 D I0_ 10 O I iD I 10 10 6 . JQ 10 10 I010 10 ID I lb 10 10 l0 _ _ _ _ m a0 ' oI to to 10 to /O to 76 J I 10 CC) (1( slC) IO ,l 0 _-lO . A Pan weight (mg) _ _ _ Color Idetlcto: X 1jt.,r 1e1.-, J6.21 1. ol 1.5.11 1 Ziz Iq.j I Iq.SO 1v*s IS. 10 1q.9" I .31 Analyst: B -Pan + Larvae weight Anaglyst: .la3aR a ma,+ U; A10 ls 2%1*1f43A3

                                                                                                                                           \6a Larvae welght (mg) - A- B b,8        55t.us           (t.,S 1's31.92        A7.2           ,5            .        s          n           z Weight per Initial number                                                                                                      _

of larvae (Mg) .4 .. _ ClInitlainumberoflarvae ° ' O* °' t O OO0 I Average Percent weight per reduction 0 .60_q Initial from control number of (% lar v ae (m _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ __ _ _ _ _ _ _ Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

e-V, Environmental Testing Solutions, Inc. Page 4 of 6-'-5

. I.. ..

Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: n-l-Ct)S Survival and Growth Data Day 100% Intake -

                                              .__                  Y         Z I AA         BB 10       10      IO      I/

2 1t 1 10 /0 3 _ _ _ ((0 10 10 4 to

.5

_ - - ID 10 6 ID 9 10 7 .lo 1 A- Pan weight (mg) L. Color Identlfl tlon:-A, .2Ai I.Az IS.23 j&..39 Analyst: r Z B P Paa + Larvae weight (mg) Analyst: Larvae weight (mg) - A- B Weight per Initial number oflarvae(mg) . '.0 C1 Initial number of larvae At O ) Average Percent -e weight per reduction Initial from control numbero- (%) larvae (mg) O. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m - missing, sk = sick, sm unusually small, Ig - unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: -re S t

                         '      7;e~t      GCr6PMMS            Ue        v6our~bFy

TVA/ Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26,2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephatespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Clieat TVA/ Sequoya Nuclear Plant, Outfall 101-Nan-treated - Test dates: July 19.26, 2003 Project number ,991 Revehwed JM~in C-' i--r A,....t WIAIn. I Ch~an~('X Ceaw (O) 8 i

Ia . .- t Cat .. *.f h A-_IekS ( _b 8- u"a L. ( u W.U*h 9..b04- MW- u.ig*I*-4 Cu.Irk .t* t -twit t*.e.4 45 he( . . -A. * -A rtla .. 0.. a1.eaf ... c) N he.'a (._ ( afthu... P.uP.WP ..n.I (%

A 1 0 10 16.24 23.20 6.96 0.696 0.696 Control II 10 to 16.24 23.40 7.16 0.716. 0.6t1 6.t 0.716 100.0 0.66t 6.8 Not applicable C to to 15.14 21,27 6.13 0.613 0.613 D 10 10 14.95 21.93 6.9S 0.693 0 698 B 10 to 14.86 21.87 7.01 0.701 0.701 to."% - tO 10 16.32 23.61 7.29 0.729 0.714 4.2 0.729 100.0 0.714 4.2 .4.8 G to 10 16.21 23.00 6.79 0.679067 UH 10 30 36.30 23.76 7.46 0.746 0.746 1 10 30 14.72 23.47 6.75 0.675 22% 310 30 16.19 23.17 6.98 0.698 0.680 4.1 0.691 100.0 0.680 4.1 0.2 IC 30 tO t6.28 23.31 7.03 0.703 0.703 L 10 10 16.31 2273 6.42 0.642 0.642 M to 10 16.33 23.11 6.78 0.678 0.671 U39% N 10 s0 14.75 21.30 6.55 0.655 0656 3.4 0.655 M.0 0656 3.4 3.7 O I0 to 16.23 2288 6.65 0.665 0.665 P 10 30 15.09 21.34 6.25 0.625 0.625 Q t0 30 15.1l 22.24 7.13 0.733 12% R 0o to 16.28 24.20 7.92 0.792 0.146 4.7 0.792 tW.0 0.746 4.7 .9.6 N 10 10 14.9132217 7.26 0.726 . . 0.726 T lo T 1 :418 73 6 sz 0 0.53752.1 U to lU 13.10 7.51 0.7 1, 100% V I 110It t S.0 1 21.63 I 6.53 f 0.653 0.695 6.0 0.653 800.0 ILO Act W l 10 t0 1 14.99j 21.76 1 6.77 I 0.677 0677 30 l0 16.37 23.36 6.99 0.699 0.699 0.104 ________ 1-I" 9 0.660 925 4.8 100%1WAk 0.699 5.6 0.648 14.3 0.514 0.715 o trall let: MSD - Minkim Sipifiunt Differance Dusett's MSD value: 0.0592 PMSD - Percent Mmknsn Sigpifnt Diorenceo

 .MSD:                         8.7                                                PMD50  i anwmea of tat preuckl           The MDtstb the ninihnIm percent diffemnc betwen the cntrol end trusbnot that ca be declared statiticolly viificnot hi a -hole emnunt toxity teat.

Ona* g *aipi t diffrence occm cfcEntvironerntateteingSolutmo, leo. crohi tokichytlsh when a toxiet rduces Ptpholes goh by 13.4% fiomthentrol (detrenned Intake: thru retarence tozicant leaSins). Dausette' MSD value: 0.1005 LowerlMSboanddeternmedbyUSEPA(IOth peretie) -9.41 PMSD: 14.8 UpperPMSD bond detined by USPA (90th pt til) -35K Th lowetnodupperbound werecalcuatd bythe USEPAwsg205 tueaodutted dromnt 9 bbortories forlmephales 1 hI chrnicrefernce toxiant teats. USBPA 2000. thdiWdinud ounting for Method Vaiabty iWhole Effluat Toxity Applia Under theNaina Polutut Dscha Elirneioo Nipm EPA-133-R-00003. US Eavio taPtotedion Agec Cimncnati 01

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Statistical Analyses Luvt r~I Cis., sad SorviTeat.7 DsyCr..r SunDate 7119r/205 TtID, PpFRCR SmplelD: TVAISuhNucierw nt.O R 101.N n-tated d Dte: 7/262005 Lab ID, D Eavr. aTaft Sat B Smple IYPC D&.DdipsRs]Wp pot Sakl Date. Pwtocot PWFWCtR-PA21.R42-013 TedsSpecie: PP-hopbaes pneleu C"a_% 1 2 3 4 D-Concol 0.6960 07160 06130 0.69t0 10.93 0.7010 0(7290 0.6790 07460 22 0.6750 0.6910 0.7030 0.6420 43.9 0.6no 0.6550 0.6650 0.6250 72 0.7130 0.7920 0.7260 0.7530 100 0.7510 0.6530 0.6770 0.6990 Csse.% Mess N-Mes Mess Min Mal CV% Y N I-St Oi"l MSD Mea N-Mesa D-Col" 0.610 1.0 0.6101 06130 0.7160 6.765 4 0a6973 I.OXO 10.91 0.7138 1.04t5 0.7138 0.6790 0.7460 4.15 *4 *.1343 2.410 0.0592 0.6973 1.00 22 0.6795 0.992 0.6795 0.6420 0.7030 4.093 4 0.051 2.410 0.0592 0.6941 0.9954 43.9 0.6558 0.9633 0.6558 06250 0.6710 3.440 4 1.017 2.410 C00592 0.6941 09954 72 0.7460 L.09S9 0.7460 0.7130 0.7920 4.671 4 .2.655 2410 0.0592 0.6941 0.9954 100 0.6950 1.0209 0.6950 0.6530 0.7510 6.013 4 .0.380 2410 00592 0.6941 09954 AusvIlsryTeT Snttioe Cttles Skew Ktrt Sbapwro-Ws Tat 3ndmata noemal dnI-utvn (p > 0.01) 097tJ6490 O914 .027199037 44161453 BNdWks Test ktdxats equal viaca (p- O.t7) 1.32396853 15.01627224 Hyll lshw6Tel(.h0.0S) N4OEC LOIC EkV TU MSDs MSDp MSB MSI -PFrsb if D=netfiTest 100 >100 1 0.05923351 O.07022236 0.003949075 0.001211itl 0.0213543

5f1 Tr t "vsD-Cmifrol Llr.arlatrp.letd (20 Reles) point % SD 95% a411p) Skm IC35 >100 ICIO >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 IC50 >100 0.7 0.8 0.2 0.1 0.0 .!. **. s

                                                                                                     -0.2 0      20       40         0o     sO       100        120 Do %
                                                                                   ... p D0°J39          s.                                      .

14*8. 0.05 Iel 0.6 .. .. ...... ... .... ..... i^r= 0.5 0.4 0.2 C.1 0. 3 8d spJ07-19-05datr

TVA I Sequoyah Nuclear Plant, Intake Non-treated July 19-26, 2005 Statistical Analyses Lu-d FimbCGwn and Sr+awl Test-7 Dsy Crrwtk StatDec 719r=0T5 TntlD: PpFRCR Sampl D1. TVAl SqhNuckarPlat.IntakIe -N4=4-d En Dl) 7/26/ S 1 lAf. ETSavwk.Tatig s50 Sampla 7e: DMR-Dschage M.onitrn Repoit Sample Dute: rtoott WFWCRHEPA-12l.R0213 Tart Species ?P-PThbaks pr hsnek Cew-V. 1 2 3 4 DCol 06960 0.7160 0.6130 0.6980 100 0.7040 0.6600 0L.140 0.7150 Tr femnnwUnramtsruod I.Tclbd isatic CONc.%. Mean N.MM. Ma" Mil Mat CV% N a MsD Ma. Meant N.Mmm D-Contol 0.6t08 1.000 0.6t0t 0.6130 0.7160 6.765 4 0.6S08 1.ODO 200 064n3 0.9523 0.6J43 0.5140 V7IS0 14.5 4 0.629 1.943 0.1005 0.6423 0.9523 AzziliaryTests Sbtkdc rIcal Skew Ken Shapio-WilbTatidica notmaldar buton (p)0.02) 0.114729613 0.749 .1353771 1.253381696 P-Tat bdkato equalvalkwn (p - 0.28) 4.043024063 47.46722794 Hypothes Tat? (IcbM 6.) MSDv MSDp MSB MSK 1.2re 6f Ha.omoatr0Ta ztdtamdtasnou igamn difftro 0.100412498 0.147605579 0.0021125 0.00534797 0552t33319 16 Tratmt we-ontrol LU.e.r ls.trpolath (200 Rmampta) P.t % SD 95%VCAErp) Skew ICQ5 >10 tC10 >10W ICis >100 1.0 1C20 >00 0.9 IC25 >100 IC40 >2W0 0.8 tC50 >100 0.7

                                                                                              *0.4 03 02 0.1 0.0.                       .    .   . .  .    .   .

0 20 40 s6 80 1M0 120 Do" 0.0 0.7 0.6 0 .2 14a20.Os o( ~gtg~fcarnc. 0.5 0.4 0.2 0.1. O0 ws s07.1 9OSdoaa

I . *- EnviaTg S Environmental Testing Solutions, Inc. Page I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphniadubia Client: TVA County: Hamilton Facility: Sequovah NuclearPlant Treatment: Non-treated NPDES #: TN 0026450 Outfall: 101 Project #: IA&i I Dilution preparationinformation: Comments: Dilution prep (%) 10.98 22 43.9 72 100 Efuent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 '700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: l Organism age: , zA-90-3S.& OQt: Randomizing template: q.LnuU3b Date and times organisms riI-c15 oMOS -M 1OL4. Incubatornumber and I were bom between: . shelf location: ZZ43 Organismsource: ai- VZ-oS A-k YCTbatch: 042545 Transfer bowl information: pH .8.7- Temperature - Zs.2 Selenastrum batch: Ut3* al-Ib-OS Daily renewal information: Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time MAS 0 a1 -A-S Air &. - 05 6 cr-16 0sra1ltt.c 0 94O2 2r 7n-0* jQD 3l- tbS6 OscrlR.o0 CO2Z. c2-,zzos JI I1 L a OIno1 .0 02 iaC0;4 a41-23-S llO2 C05 ts0os 1z2.oo 0d2. 7 s cri-Ai-OS 137Z ._____A __.__ I 6 aiizS-o 112.l ~~1007.~~

% Adults having 3Y Broods: 10% k 80X . NOEC IO% % Mortality:- 077. s 20/. LOEC IIt'7.
~~Mean Offspring/Female: 2aA 29.'4 2 15.0 offspring/female ChV > 1007.~~
~~%CV: S7.37 T17 c40.0 % IC25 > I1C017~~
Environmental Testing Solutions. Inc. Page 2 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: 0l-7-A-I CONTROL - 1 Survival andReproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 l0 I Youngiprodu1ed O +/- r Adult mortality 2 Young Producedr I o 0 5 O . CO Adult morhailty L- I , L- L- L- L- 1>
~~- _1 _ = -~~
3 Young produced 0 C QO O Cl - l0 V : T 0 Adult mortality l - I .- L 1 7i - 7 4 Young produced Adult mortality l9 LA L._
~~.22 37IT L-L- L- l - l -- lC I~~
s Young produced 9 jq l lotq Adu lt rt. Cm Ci L- I- C. t- I `1. 6 Young produced Adultmortality CC 0-.- '-- I's I) o ,-t TIs t 7 Young produced Ito 11 0 I11s Ia 0 0 Total youngprodueed l ll l8 I ZS l 7- lp0 l 2 2 Final Adult Mortality l l IL C - L Xf Broods X. - - I % .1 I Note: Adult mortality (L - live. D - dad) CConcentration: I l %Mortality: l eY I Mean Offspring/Female: g 1 CONC: 10.98% Survival and Reproduction Data
- - Replicate number I Day __ _ _ _ 1 2 3 f4 5 7 8 9 1 lI lYoung produced l l()4O ) lO lC =s Adult mortality - L- L -
1 l2 :lYoung produced l l l) 0 l Adult mortality l C_ L(_ l l l l L l l. 3 Young produced - C)0 4 llAdultYoung produced mortality l S U.-C 1_, 1 i -- ILA L L.. _ Adult mortality L-'-~ L- -- %--_ S Young produced lF C l I CO l 6 Young produced 0 c) 0o I 0 0 L ItGl Adult mortality L-C l IL- l. C- 1-. C.. C.l Total young produced l 3l9 3Z l 3° 33 l Zl3 l 3 Final Adult Mortality C - l C * - - NOte: Adult mortality (L -live. D - dead) I Concentration: IMortality:: a I Mean OffpringfFemale: I % Reduction from Control: I -1. 0701 I --I. q l
~~. :.-.:- . -,.v. ";7,.M.~~
. ;.. ... .:I .11 Environmental Testing Solutions. Inc. Page 3 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: t.
q-cS CONC: 22% Survival and Reproduction Data Replicate number D __ 1 2 3 4 5 6 7 8 9 10 I Young produced 0 l ) t A ( O) C O _ Adult mortallt _ _L-L L 2 Young produced 101 l O n o O o Adult mortality . T - 3 Young produced O 1 DCO) 0 O Q. c o Adult mortality L L +-L L.- L-- L 43 Young produced Young producedl Lt*.. C I . 1.
... . I 0QS LA qC 774q e Adult mortality 1 1 (L- %--. _. L I 6 Adult mortality Young produced o O O CO IS C> -i l Adult mortality L_ L,. . L-. L..- %,- ! ' .
7 Young produced LLP2 1 Jj j1-1 Iq OI L JLC L Total6 produced oung Ttlyug rouej 3z 3 CzZ 3 S 0S :5 3li *3t S ll 7-9t 3 1 1 .30 .32. Flnal Adult Mortallty, 1 U l 1 l L t Note: Adult mortality (L live, D -dead)
- Concentration: % Mortality: 0 I Mean Offspring/Female: I 3\, %Reduction from Control: - .ST7e CONC: 43.9% . Survival and ReproductionData - ~~Replicate number - - -
~~DaY~~
17~ 2 3 4 1 6 7 a 9 10I l Young produced l ) l 0 C p O (
Adult mortality U C1 U L I ~ . .U . 2 Young produced l l _ fr C) 0 l Adult mortality - U C I U L T 7 L 3 Young produced lU) lQ *l 0 C) £). 0 - Adult mortality .. ~ U T U .U Young produced l S l tL l SI 4 L1 l 5S YoAdult Young mortalio producedc l-ll :fZ I l\1l1I O L- Is I-L. I -n l 19L lZ - L FAdult mortality AL 2_3j = L12 L I _Z 7 1 Young produced [ 1=9 16 a- ( -s Total young produced l 31 l 35 l l Ih3 Flnal Adult Morblity l-l- I § l - U. '- VUM. ^Aulttmortality (L-live.D-dcad) - oj exe.A I Concentration: Mortality: Mean Offspring/Female: 1 0%7 l 33. l I % Reduction from Control: I - I g.O Z
i FEnvirnrmental Testing Solutions. Inc. Page 4 of 7 Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant-Non-treated Date: oai-lS-S CONC: 72% Survival and Reproduction Data Replicate number Day 1 2 3 4 S 6 7 8 l 9 l 10 I Young produced O j O _ O _r) Adult mortality . L _ L '- 1 Young produced O 2C) O C _ O C) - Adult mortality 7 - L L Young produced - - O - - __.O_ Adult mortality
~~L ( L-U t L 4 Adlt produced Young rtlty~~
_%__ I-I L- - V L_2* L-- 3 ŽL- I L- 1 L Adut mortality _ _. l t 6 Adult mortality Young produced _ _ _ L > 0z 1.- 13 _- . L- I L-19 L. 1 6 Young produced 0 0 .;. ZO I)1 0 O _ _ Totalyoung produced .3Z 31 31 1 33 33 l 3L l30 a FinalAdultMortality . : ' ; . , , . 2 T _ l *t Note: Adult moalityj L live D - ead ':'_::
. <4lConcentration: %Mortalityr 07
~~.. Mean Offspring/emale: 13.~~
%Reduction from Control: IL .S7 0 CONC.: I 00%07 r ;W r. . Survival andReproduction Data *Repliatneumber Day ,. '-^ --... "'- l - 3/4U' 2 i 3 4 l 5 6 7 l S 9 l 10 l lYoung produ~dl........ O lO lO l l lC) O O. O C) 1 -lAdultmrwor;-e i 6 .
Young produced -l l0S . 3 Adult mortality Young produced l i 'O1 ..
~~L- . L 1 Adult mortality - U' l l ll TJ~~
~~- Adult-ort L. l L L-ty L- L 46 Young Young produced.~~
~~Youn~gproduced produced o 0DN - 0~~
~~°l() I 1- i3(L Li 1 (3 -27)~~
I's ) ~ Ts Adult mortality Adult mortality - l L NU . j llIIl LU CLl ~ L 7 Young prod uced jt - - _ _ - 1'1 Ttlyug produced 0 0 . 3.m2 37 z ult Adu t oraItyLAd . .. - U. U Note: Adult mortality (L - live, D- dead) l Concentration:
~~%Mortality: (V.~~
~~I Mean OffspringWFemale: 123. £,~~
~~%Reduction from Control: -ta.37,~~
I.. .^ , Environmental Testing Solutions, Inc. Page 5 of 7 .. : Species: Ceriodaphniadubia Client: Sequovah Nuclear Plant - Non-treated Date: O1-l0-Os I CONTROL - 2 Survival and Reproduction Data Replicate number I Day I Young produced Adult mortality 1 Q
\
~~Qi j) 2 QO L 3 L* 4 l 5 6 7 l 8 9 10~~
~~2 Young produced O ) _ O_ OO _~~
Adult mortality L E C - L 3 Young produced OO7-OC Adult mortality7 _ _ L 4 Young produce d 5Ll -5 Li 5 5 5 'A A-ult mortality _ L- L-S Young produced l 0{O9l Adult mortality L - - 6 Young produced O O O l Adult mortality L. L- L L L- L L 7 Young produced [I IL0tC) 5 I's 0 O C .1(.. Total yougng produced 3 - Zq Z . Z 3 Final Adult Mor orlita L; L Note: Adult (L live, D dcad) -ortality
- Concentration: .<- lMean Offipring/Fernale: Z9.;1 CONC: 100% Intake ; .and Reproducti'onData I Young produced r l l r9 0 0 Final M wdult mortalAty L Li. L i- L . Li.. . L Li1 l2 lYoung Adut produced m rtlit lO 1L- lOl<. 2 *i. =.3 4 I 5jl(
Mea 6 Of 7-S emle 2'- Nate: Adultdmortalty lI'L t lie dI 35 l Young tproduced Adult mortality l L l L-' 1R L. L Al.' .. e t vCnl eprodu L -I.. to %u-Data L- ~ s Young produced .. ) 4)1 lo.. to,.L~ C-l Adult mortality Young produced l Li*4 l* Q 4 L-LL QO LiL-Dl . L-7 Young produced IIzS L. :i - - -w* l Young produced ro ou ne l L J ) . r C - o_ Adult mortality C T T 7l IJL lll 6o a Young proucd uced ( C) 0 _ _ 9 05 Final Adult Mortality i U. L t i I tW .. I Note: Adult mortality (L - live, D - dead) I Concentration:
% Mortality: O -Mean Offspring/Female: l 30.5 l-%Reduction from Control: l-.7
TVA I Sequoyalh Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Verification of Cerladaphnia Reproduction Totals Control-I 72% Day ieptiW 5 ' 7-j--ber ~ j Total Day Re lIcate 5 nuWmttr 6 7 89 10 Total I !2 3 4 1 0 0 0 0 0 0 0 0- 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 00 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 6 4 4 4 5 4 4 4 4 44
-5 9 9 9 30 9 10 10 11 9 9 95 5 11 11 12 13 12 12 11 10 13 10 115 6 1I 0 0 0 15 0 0 IS 13 16 60 6 0 0 0 0 0 0 8 13 7 19 16 70 7 16 14 16 17 0 15 13 0 0 0 91 7 _191611620 1516 000 0 .302 Total 30 271 231 311 28 23 23 30 26 23 284 Total 135 331 32 37 31 33 3 31 6 30 331 10.98% 100%
Day RepIi vate nunherToa Day Replicate nuiter - - Total Dy 1 2 3 4 5 6 1_ 7 9 0 Toa 1 2 3 4 5 6 17 3 9 10 1I_ 0 -0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 0 2j__ 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
-3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 4 5 4 4 4 S 6 45 4 4 4 5 4 4 4 6 S5 4 45 S 110 10 10 10 I11 10 9 10 10 101- 2 1 I 10 11 I 1 13 10 12 11 114 6 0 0 0 0 17 0 0 14 18 16 65 6 0 0 0 0 0 0 0 0 20 19 39 7 16 15 13 15 0 15 19 0 0 0 93 7 17 -F-171917 13116 15 19 0j 0 133 Ttl 3 2927 29 3230 33 27 33 32 304 Total 133 34- 34 32 331 31 34 3L4 37 34 336 22% Control-2 __ _ _ _ _ _ _ __ _ _ _ _ _ _
Da2 Replicate inumber 3- -9 1- Total DyRep Icate nunter Total 273 43s ' 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0- 0 0 -0 2 0 0 0 0 0- 0 0 0 0 0 ___ 3__ 0 0 0 0 0 0 0 0 3j 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 3 4 4 4 40 S 121 11 It 10 12 10 10 10 11 11 103 -t1 3 9 9 30 10 I30 9 9 95 6 0 0 0 0 15 0 0 17 15 17 64 6 0 0 0 0 1 0 14 13 17 14 74 7 16 17 19 107 0 14 36 0 0 0 9 7 116 171 015 0 0 0 7 Total 32 321 351 31 _31 1283 291 31 303 11 Toal 31 31 30 29 29 2 29i 2 31 27 29 43.9% 100% Intake Day 2 1 0 0 0 0 0 0 0 0 Rep2licate nlunter 0 0 5 6 0 0 7 0 0 31 - 9 0 0 0 0 1 0 0 Total 0 0 Day 1 I 2Replunte 0 4561789I~ 3 nunber 0 0 0 10 0 Total 3 0 0 0 0 10 0 0 0 0 0 0 3 0 0 00 0 0 0 0 0 4 5 4 S 614 4 4 4 S 4 45 4 4 4 4 4 44 4 4 3 4 39 5 1012 12 10 I1113 30 12 12 10 1112 5 11 10 10 13 9 10 13 30 10 I1 307 o 0 0 12 19 18 1 92 6 I0 10 0 5 0 3 16 0 36 48 7 19 19 16 17 0 05 00 0 3 7 15 15I 1o3 lgI 19 01 15I 0 14 0 111I Total 341 35 33 351 32 34 2 3536 132 1335 Total 30T 291 321 36 123 291 331 30 27 3 0
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia Quallty Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA/Sequoyah Nuclear Plant, Outrall 101 -Non-trcated Test dates: July 19-26, 2005 , Projed number 1991 Revelwed by: -- Concentration __ Rcate number ___ Survival Average reproduction Co n lket of Percent reducion from (M) 1 2 3 4 5 6 7 8 9 10 (%). (offsprlngl'iemale) varation (%) pootedeantroti (-/) Control - 1 30 27 28 31 28 28 28 30 26 28 100 28.4 5.3 Not applicable 10.98% 32 29 27 29 32 30 33 27 33 32 100 30.4 7.6 -7.0 22% 32 32 35 31 31 28 29 31 30 32 100 31.1 6.1 -9.5 43.9% 34 35 33 35 32 34 29 35 36 32 100 33.5 6.2 -18.0 72% 35 33 32 37 31 33 33 31 36 30 100 33.1 6.9 -16.5 100% 33 34 34 32 33 31 34 34 37 34 100 33.6 4.7 -1S.3 Control - 2 31 31 30 29 29 2 29 29 28 31 27 100 29.4 4.6 Not appicable 100% Intake 30 30 27 31 100 30.5 8.6 -3.7 Outrall 101: MSD - Minimum Significant Difference Dunnett's MSD value: 2.014 PMSD - Percent Minimum Significant Difference PM*SD: 7.1 PMSD isa measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in awhole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when intake: a toxicant reduces Ceriodaphnia reproduction by 9.2% from the control. Dunnett's MSD value: 1.624 Lower PMSD bound determined by USEPA (10 d percentile) - 11%. PMSD: 5.5 Upper PMSD bound determined by USEPA (90' percentile) - 37%. Tle lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphniareproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati. 01 l.
IVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Statistical Analyses C.oci~ehals SurvtuJandRtprodac.6 Tut4teprodu.cti Stt Dat: 7119/090 Tat 1ID: CdFRML Sample ID: VA/ Sequoyh Nulear Plbnt,Outll 101. Na.etre EcdfDate: 7rs LabID: E EniTestitgSol a Sample Type: DM R.DMchwVcf kgctapm Sample Dat: Potocot FWCHR-EPA4121-R4.013 Tat Species: CD.Cvdaph" dubk Camentr CaC.Y. I 2 3 4 S
7 a

1 D.Cmtml 30.000 n.ooo 21.o0o 31.000 23.00 21000 2 .000 30.00 26.000 21.000 10.9 32.000 2 n0eo 27(0O 29.000 32000 30.000 31.000 27.00 33.000 32000 22 32000 3.000 35.000 31.000 31.000 21LO0O 260O0 31L.OO 30.000 32.000
43. 34.000 35.000 33.000 35.000 32.000 3400O 29.000 35.000 36.000 321000 72 35.OW 33.000 32.0D0 37.000 31.0L 33.000 33.000 31.000 36.000 30.000 100 33.000 34.000 34.OO 332.000 D30 3100O0 34.000 34000 37.000 34000 Trulofra:1 v Umateaf4.d I-Tsiled lsetik Cme-.% MIem N-Mess Me" Mb Mar CV% 0-Stat <idnl MSD MfeDa NMesD D-Cznml 23.400 1.0000 21.400 26.000 31.0 5.301 10 31 6L3 l.000 30.93 30.400 1.0704 30.400 27.000 33.000 7.621 10 L270 2.27 2.014 31.603 l.ooo 22 31.100 1.0951 31.100 21.000 35.000 6.141 10 *3.065 2.217 2014 311433 0.0000 43.9 33.500 1.1796 33.500 29.000 36.000 6.174 10 .5.790 2.217 2014 31.613 0.00o 72 33.100 1.1655 33.100 30000 37.00 6.197 30 .5336 1227 2.014 31.613 l.o0 100 33.600 1.181 33.600 31.000 ".oo 4.69S 10 *5.903 2.217 2014 31.613 1.0oc Asrtubx Ststk Olcal Skw Klrt 1Ctarju~YD Test stats noOCma08n6,tchf(p> CL01) 0.509913653 1.035 -Q053 1424 -0.27574359 Bartlet, Test indicates eqmlvcea (p -073) 2.774007797 15.01627224 lvlythes Twt(lXbil, 6.06) NOEC LOIC ONv TU MSD. MSDp MSB MIK F.4rb it Doemetr Test 100 >100 1 2.0142420S 0.70924233 43.4966667 3.t7s62sa 1.90-07 5.54
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TVA / Sequoyah Nuclear Plant, Intake Non-treated July 19-26, 2005 Statistical Analyses Caidaphis SsrvfvJ sad Repredertla TatRteprododcts S~t Dte: 7/19/2005 Tnt ID: CdFRC Sampl) ID. VAI Saquoyah NnkarPkn Inlah- NM-trWd r Date: 26/2005 Lab D TSEnv. Tetki Sol Sampe TYpC DMR-Dbc uFaro Mwoh cpmt SompleDate: Protocot FWC8R-EPA-U21R4024013 TestSpecks: CD-Curdopbnsdubie C~RsC% 1 2 3 4 5 7 3 to D.Ctol 31.000 31.000 30.000 29.00 2.000 29.000 29.000 23.000 31.000 27.000 100 30.000 29.000 32000 36.000 21.000 29.000 33.000 3000 27.000 31.000 Theess tlfruuier~ I-T iled Iils~ Caae.% Ue. t4Mes Mess Mb. Mel Cv% IV 0-361t 1 . 7tSD Mess NMes D-Control 29.400 1.0000 29.400 27.000 31.000 4.591 10 29.950 1.OOO 100 30.500 1.0374 30.500 27.000 36.000 .640 10 -1.175 1.734 1.624 29.950 1.0000 AuxI~oryTeefs Masis.e QMIC11 Skew Kurt SbaprO.W&kl Tet Wwatmw -1 dskl.tm (p > 0.01) 09440490.5 0a 6 0.799423706 1.55296927 P-Test bdicates tqutnrine (p - 0C06) 3.110975552 6.541089535 Ilypestes Test .os MSDv MSDr MSB M 4lt F-Preb di omioscedutictTestkdicato nosipifrnldufdmee 1.A23612015 0.035224901 6.05 4t3333353 0235370061 1. 1 T.uunntmais D.Cocfrol Lisest Interpolato (DO0Itasels) PIsot % SD 5% C Skt IC0S >100 ICIO >100 ICIS >100 1.0 IC20 >100 01 IC25 >100 IC40 >100 M50 >100 0.7
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Des.-PespemsePWCs 40 35 30 ._ F 4.8 0.06 k Af I 25 .
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Species: Pimephalespromelas I Client: TVA County: Hamilton I Facility: Seguovah Nuclear Plant NPDES #: TN 0026450 Project#: 1qcl Treatment: UV-treated Outfall: 101 I Dilutionpreparationinformation: Comments: Dilution prep (%) _ 10.98 22 439 72 100o Each concentration was treated I E~uent volumc (rnL) Diluentvolurnme(mL) 274.5 2225.5 550 1950 1097.5 1402.5 1S00 700 2500 0 for 2 minutes with a UV sterilizer to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. I Test organisminformation: Test information: Organism age: 1z ,- *b.S 1oJS. OLb Randomizing template: tUe I Date and times organisms were born between: tn it a0bos 'O M AScO Incubator number. Organism source: ASS. GAvv cSI- te-QS Ariemla lot number 1rlQ I. Transfer bowl information: Average transfer volume: pH 1.S9. Temperature = Zql-C Total drying time: Date / Time in:% z6-C3 Date/ Time out: oc-zi. os i3 l O 83 I . Oven temperature: l* C.' Dailyfeeding and renewal information: I' Day Date Morning Afternoon Test Initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used I - Cn- ls-oS time S36 termination time
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6 O1S-tlS 0k'; 7 j 1SS 1211 crpr PS 2n0.ptIOZ- A 7 - - - _ _ Controlinformation: Acceptance criteria Summary oftestendpoints:
%Mortality. 07t S 20%*/ 7-day LCso > 1 7fO Average weight per initial larvae: o.16b ; 14rM 4-z NOEC 4 to.
Average weight per surviving larvae: 0.16 1 2 0.25 mg/larvae LOEC >1 OI . ChV > Ico/. IC25 I> 1067%
Environmental Testing Solutions, Inc. Pageoc~ Species: Pimephalespromelas Date: 01- tll- oS Client: IVA I Sequoyah Nuclear Plant - UV-treated Survival and Growth Data Day CONTROL 10.98% 22% - A B C D E F G H I J K L 0 _10110 10 10 10 /6 10/o /6 /0 10 _ '0 10 10 IC 10 I 0 10 /0 I0 /0 '0 2 10 0 0lt 10( 6 0 / 10 I)t10 3 -lbIt 1010 _ 0 1010 10 /I/O 16 4 _ _ to I_ ) o tt_ o t I0 I0 io l/0 loII l 5 .- ID 10 10 I0 10 l0 10 la /0 6 _ lb 1. 1 J _0/ / /0_ t 0 7C 101 0 to 10 to 'IC10 /0I Jo /0 I0 A - Pan weight (mg) t51_ _ _ CololdstIy,4 Analyst' : 1q. 21?Z , IS.,2
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t O' DI 0* O! O* Average Percent weight per reduction Initial from control Wub k -.1' -%. ,t, 1'.3 -I % number or (%)) larvJe (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:
Environmental Testing Solutions, Inc. Pagc3 of6 . -.
*1.S Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: M- ig-OS Survival and Growth Data Day 43.9% 72% - - 100% -
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. ,74 1.145 1.,48 1.1* - 1.8111 d 1.11Z I-,St ,Ioz (..& J..l q ,zl Weight per Initial number . A _ . -
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Average Percent weight per reduction Initial from control bO O.1 0.1 .I.-, 1 -. number of (%) Larvae (g). Comment codes: c = clear, d dead, fg fungus, k killed, m = missing, sk sick, sm unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:
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Environmental Testing Solutions, Inc. t! .. Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: 0711q'OS I . Survival and Growth Data Day 100% Intake-I 0 1I Y Z O'o AA 10 BB lO I-1 /0 /0 /L JO I. 2 - 16L (* ()o i ... 3 JD 1 /0 10 i' .4 to /O {6 / 5l1 10 D10 1 I.; 6 lo )O 10 iO i 7 A - Pan weight (mg) i6oto5 7 LO 0t .4
. ;oor Ien Illptlon: ge5I 21.I5.ISZO 8 Analys :4z B - Pia + Larvae weight (mg) , 30 Larvae tweight (mg) - A -B Weight per Initial number oflarvae (mg) t ) -C/Initial number orlarvae te O .\ a.
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Average Percent weight per reduction Initial from control 66 l0 I z.0% number of (%/6) larvae (mg) Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.
*Calculations and data reviewed: 4 Comments: ' t I-A - b I ' r 4~~~~~~ .' FCIA~tS^tsv4Sto-s FI-at._t
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated July 19-26, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R.02-013, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, snd Statistical Anslyses Client: TVAl Seqaltysh Nucler Mlaet. Outrun 101 -UV trtated Test dates: July 19L.26200 Project number. l99 Revelwed by:rn /, 4, Not for Oompliance Assessment. Intersal Labodtory QC
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s tt _ m f l l . . a A . d .1 L. A 10 10 14.99 22.11 7.12 0.712 _0.712 Control t 10 tO 16.26 23.67 7.41 0.741 0.761 6.5 0.741 100.0 0.761 6.5 Notsappliuae C 20 to 14.94 22.55 7.61 0.763 0.761 10 8.22 .O2t 0.82t _ D 10 16.24 24.52 E 10 10 15.22 1 23.20 7.93 0.798 0.79t 10 10 14.82 22.10 7.98 0.798 0.746 8.2 0.791 100.0 0.746 8.2 2.0 10.91Y% F 0.700 C 10 10 14.94 21.94 7.00 0.700 It 10 10 15.42 22.28 6.86 0.686 0.686 I 10 to 14.97 21.97 7.00 0.700 0.700 10 30 15.17 21.94 6.77 0.677 0.703 77 0.677 100.0 0.703 7.7 7.5 22% J 0.656 K 10 10 16.19 22.75 656 0.66 .6 L 10 10 1525 23.05 7.30 0.780 0.70 M 10 10 IS.Ot 22.82 7.74 0.774 0.774 34.44 2.99 7.4 074t 0.760 2.0 0.743 100.0 0.760 2.0 0.1 41.9% N 1o 10 0 10 tO 14.44 21.92 7.48 0.747 0.748 10 10 14.94 22.86 7.92 0.792 0.792 10 15.03 22.91 7.88 0.73t 0O77 J 0.7t 97.5 .7 ].1 7 72% R 30 S 30 10 16.2 2357 7.32 0.3272 T 30 9 15.21 22.72 7.531 0.834 075 0.720 U 30 l0 14.85 22.03 7.20 0.7207 0.720 0.720 v 10 I 0 I 15.31 1 22.19 1 6.88 1 0.683 0.765 13.8 300.0 0.765 13.8 .0.6 200% 0.731 0.731 W I 10 I 10 1 14.85 1 22.19 1 7.31 1 to to 14.99 24.20 9.2 0.9213 0.921
.t.-.---i.-----q - r - ... I P 0.670 5 8.5 I 320 15.22 21.13 5.90 0.662 8.1 0.596 92.5 00% talske l 1 22.20 22.20 7.06 j 0706 0.706 0.724 0.724 8.1 0.706 0,72Z : ,2* 0.6i0 8.5 I 2.0 22.42 1 21.40 1 7.22 1 0102 0.654 6.54 7.127 MSD - Minim SiPIfiecant Difference Duinets hISD value: 0.1016 PMSD - Percent Miimum Significant Difference PMSD: 33.4 PMSD Is a measure of "estprecision. The tMSD h the minimum percent differencebetweeni the control sad treatment that can be declared startstily SInificnt In awhole effluent toxicity test. On veragea siganificant difference occn rOe nionmental Testing Solutions. Inc. chric toxkity tetswban tolicant redue Pmephates owth by 3% fm the control (determined thugh Intolwt: releraee toxiant tosting).
Dumell's MSD value: 0.0732 Lowet PMSD boud detmined by USEPA (10th peretilk) - 94%. PMSD: 9.6 Upper PMSD bound determined by USEPA (90th perventile) - 35%. Tbe lower sd Iper bounds were ca hlated bythe USEPA using 20$ lets conducted from 19labortoties forPiephales powtSb[ichronic re lrcnce toxicant tests. USEPA. 2000. Unde dingad Aeounting or MethodVusabiglq in Whol E nflientTolkityAppcations UndertheNtional Polluta Discharge Elimation ProptL EPA33.R.093. US Environmental Protecon Agen CicIuati. OH.
TVA I Sequoyah Nuclear Plant, Outfall 101 UV-treated July 19-26,2005 Statistical Analyses Larvvi Fbi Griw ud S4 vsl Tmt.7 Day Growth Sum Date: 7/19nco TetID. FpFRCR Smylek M. IVAISaqyaNclrarntruhWIOl..- utreed Eod Datc 7MM2005 Lab ID. EM2vk. Tetg Sol Sample1 7DPc MR .Di.ch-s MonbitdgRepot SapleDate: Protooot IPWHR-EPA121-RP24013 TedSpecies: PP.?hapharsp iss Comment Co.' 1 2 3 4 D-Control 0.7120 0.7410 0.7610 03280 109o 0e7910 0.7910 0.7000 0.6160 22 0.7000 0.6770 0.6560 0.7300 43.9 0.7740 0.7450 0.7410 o.7m 72 0.7920 0.7100 0.7320 0.7510 too 0.7200 0.630 o0.o 7 0.9210 Tru rfu Ustrvutd t-Ti;hd
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vn Stft CH"a1 MSD Mea NMda D-Col 0.7605 1.O 0.760 0.7120 0.1210 6A,1 4 0.7605 LOWO 10.9o 0.7455 0.903 0.7455 0.6e60 0.79S0 2.163 4 0.356 2.410 0.1026 0.7479 0.9234 22 0.7033 0.9247 0.7033 0.6560 0.7e00 7.711 4 1.359 2.410 0.3016 0.7479 09r34 43.9 0.79 0.9990 0.759e 0.7450 0.7740 2.023 4 0.016 2410 0.1016 0.7479 0.23J 72 0.7658 1.0069 0.7633 07320 0.7920 3.300 4 .0.125 2410 0.1016 0.7479 0.9134 0oo 0.7650 1.0O59 0.7650 0.6980 0.9210 13.302 4 .0.107 2.410 0.1016 0.7479 0.92u AsaitleryTest StOi2 Crl Stk Kurt sSpr- sTe tes v detb l (p>l) 0.920691669 0.14 1.1962202141.926649961 tv >Tistkdkataequal vinca(p- 0.10 9.362381472 I5.0u627224
*tterbt(I.ttL .O3) NOtC LOEC CoV TU MSDv MSDp MeSS MSt FPrb d4 DuinnauaTest 100 XO1 I 0.101359139 0.1335423590.002307342 0.0035316110.665465117 531s Thbesut vnD-Contmt sur latlrpolad.. (200Roamplu) pan . SD 9S%C4Exp) Sk.'
ICOS >100 ICto >100 ICts >100 1.0 IC20 >100 0.9 1025 >100 IC00 >100 0.8 C00 >100 0.7 0.6 0.5 I0.4 0.0.3 0.2 0.1 0.0 , ....... 0 20 40 60 ea 100 120 0.9. 0.7........ 14 l 0.4 r-2 O.2
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TVA / Sequoyah Nuclear Plant, Intake UV-treated July 19-26,2005 Statistical Analyses Lavsl Fsla Cm.* ad SarvsiTw.7 Day GrCtb Sut Date: 76194o5 TMtID: 1 pFRM Saml ID,. TVA/Sequoyah Nocker flank Intar - Wh trated FMADec 7/26/2005 Lhb ID 5 r. TatingsOL SampleTy7p: DMR-Dbchap Monk-kg P R-Iot Sample Ddtcc hotocal FWCR.-EPA421-R-O2.M11 Ten Specimc PP-hmepks~p pVomelu
-Yme.% 1 2 . 3 £ D-Caatr1 0.7120 0.7410 0.7610 OAU2O loo 0.5960 0.7060 0.7220 0.6540 Treasarw:Ustmrsfrnd 1-Taled jk Cse-.% Mum NiMsa Mfefsa Min -- aM CV%/ . N tsht Icuj MSD Mean NMes D-Ctrol 0.7605 .O00O 0.7605 0.7120 0.230 6.481 4 0.7605 l.o000 *100 0.6695 0.8103 0.6695 0.5960 0.7220 .507 4 2.416 1.943 0.0732 0.6695 03S03 Avxlilsry sfetistc Skm Karl Sbapko-Wks Testlodwat doubusm (p > 0A1) 0.96156 0.749 .003401617 .1.17375 F-Tediedkates squal mn m (p- 0.32) 133502543 47.46722794 Hypetbei Test (1abi. .15) MSDv MSDp MSDB MIdE N-rb d Homos ictTe ti ddra mees 0.07318166 0.09622135 0.016562 0.002S366670.052124374 1.6 Tmrnaasta vs D-Conltol U.Ammr1es -mtespel (200 Ressple)
POWt P SD 9s%amxp) Skm ICOS* 41.716 ICI0 83.571 ICis >100 1.0 IC20 >100 IC25 >100 IC40 >100 0.° 1C50 >100 0.7
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sqw_07.19-05data
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pirnephalespromelas Daily Chemical Analyses Client: TVA/ Sequoysh Nuckar Plant, Outfall 101 -Non-treated Test dates: July 19-26, 200S Project number: 1991 Reviewedby t 1 , Concentration Parameter r_- pil (SU) DO (mg/L) Conduclqty (pmhos/cm) Control Alkalinity (mg/L CaCO1 ) Hardness (mgtL CoCO,) Temperature (C) 25.01 24.51 24.81 24.61 24.71 24.31 24.81 24.31 24.71 24.2 (SU) 7.81 7l 7.5 1 7.75 7.79 7.90 7.65 7.81 7.70 7.89 7.64 7.87 7.70 7.93 7.62 10.98% DO (mZ/L) 7.7 6.9 7.6 7.2 7.5 7.1 7.7 7.3 7.8 6.7 7.5 7.3 7.4 7.0 Conductivity Oimhos/cm) 26t8 287 275 296 270 _ -273 _ 276 Temperature (C) 25.1 24.6 24.7 24.6 24.8 24.2 24.7 24.2 24.7 24.2 25.0 24.6 25.1 24.2 pI, (SU) 7.79 7.46 7.73 7.74 7.88 7.62 7.79 7.73 7.87 7.62 7.87 7.68 7.90 7.54 22% DO (mgL) 7.7 6.8 7.6 7.0 7.6 7.2 7.5 7.4 7.8 6.7 7.4 7.3 7.8 6.6 Conductivity (oumhoscm) 255 274 260 236 256 258 264 Temperature (C) 25.1 24.4 24.7 24.7 24.8 24.5 24.7 24.3 24.8 24.3 25.0 246 25.2 24.2 p11 (SU) 7.74 7.47 7.68 7.71 7.85 7.62 7.75 7.70 7.83 7.65 7.84 7.71 7.86 7.55 43.9% DO (mgfL) 7.8 6.9 7.6 7.0 7.6 7.2 7.4 7.3 7.9 7.0 7.3 7.4 7.7 6.8
~~.Conductivity (pmios/cm) 225 241 228 232 224 226 230 ;~~
_Temperature (C) 25.2 24.3 24.9 24.6 24.9 24.6 24.9 24.3 24.8 24.1 25.1 24.5 25.1 24.3 n1l (SU) 76 7381 7.63 7701 7.8( 7.561 7.711 7.691 7.781 7.61 7.711 7.8 7.53 72% DO (mr/L) j 7. 6.8
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7.5 7.5' 8.0 J.4 6.9 7.41 7.51 7.8 68 Conductivity (pmhos/cm) 1 83 18 191183 185 ~ ~1896i Temperature (C) I1 (SU) 7.62 7.34 7.59 7.70 7.77 7.57 7.65 7.66 7.74 7.60 7.75 7.67 4-
~~)O (mgtL) 7.8 6.6 7.6 7.1 7.5 7.0 7.6 7.3 7.9 6.9 7.3 7.2~~
; ._:. 4 -_

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~~__ . _ _ _ -_ Z (umhos/cm) v _ _ 110143 1 4 -13913. 100% Ukallnity (mZ1L CaCO,) lrdness (mW/L CaCO)~~
- _ 4-rotal Residual Chlorine (mPIL) <D.IC ~<D.10 01 remnerature (C 25.3 24.5 24.9 24.4 24.9 24.4 25.0 24.1 24.9 24.3 25.1 24.6 a..-pll (SU) 7.64 7.39 7.60 7.71 7.80 7.58 7. 7. 7.66 7.731 7.62 7.75 7.67 )O (mg/L) 7.8 6.9 7 7. 7.2 7.5 7.3 7.5 7.3 7.9 6.9 7.3 7.5 Conductivity (tmhos/cm) 42 151 143 8 140 140 100% Intake Alkalinity (meiL CaCO)
Hardness (mg/L CaCO,) _ _ Total Residual Chlorine (mg/L) Temperature ('C) 25.11 24.3 24.7 46l 2. 24.3 2.8 24.3 491 2. 25.0 l 2.
EnAronmental Page 5 of 6 Envrometa Testine Tetnouin. Solutions, Inc. _nc Paeo Species: Pimephalespromelas Date: G1-119-5 Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistry: __- Dav ___ 0 i 2 Analyst A A6 Concentration Parameter 1JS i 'QK Ww. 1 4 CONTROL pH (S.U.) '1.-0 -7, jl lTs I DO (mg/L) r 1 :L1 q Conductivity 2 (iimhos/cm)_____ Alkalinity (mng CaCOA) Hardness (mg CaCOAI) ___ ___ Temperature 1C) 25 .0 - 4.5 lto %( --I pH (S.U.) -1.: B I 10.98% DO (mglL) 7 Conductivity (jimhos/cm) _____a Temperature (°C) *ZS. I 724.b 2LI.1 ?AL le '. 4 pH (S.U.) 9 ? YG . 22% DO (mg/L) 1.1 C L Conductivity (pmfhos/cm) _____ Temperature (C) ZS .1 21.4 2L .-I1. 1 I . .5 p1H (S.U.) '1.4 ?.. 43.9% DO (mg/L) Conductivity ZZ' (pimhos/cm)__ _ _ __ _ ___ _ __ _ _ _ Temperature(C) 2S .Z 2c1.3 .5- 75 *6b 4. *Z.Z44 pH (S.U.) -1. ta 39.38 V .&'3 ':I9o "4.<g 7- 4M 72% DO (mg/L) 1._ 9_.C* Conductivity I (gunhos/cm ) __ _ __ _ _ _ __ _ I__ __ Temperature CC) Z 2 A , Lt 7j%(.j IO.5 24.* PR (S.U.1 - bi r,3 .?@S9 7.67-L? 100% DO(mgfL) O Conductivity 4 '4.3 Alkalinity (mg CaCOA/L) S A b Hardness (mg C2CO)L TR chlorine (O. 10 Temperature (C) ZS.-3 2q.5 -I' pEI(S.U-.) -3 -<.Iti.?tL 01 . ... Sf K-100% Intake DO (mglL) i.g ' 1. 9* Conductivity (pmhos.?cm) LI_______ Alkalinity (mg CaCOAL) __O __2 N Hardness (mg CaCO/L) ____ TRch.10 loo i (emgCL) ____ I ._-* -
~~______Temperaturt( CC) 2 24.3 +/-J I Initial a d - Final~~
- __ L Initlal I Final .a nitlal ------ IL Final
Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephalespromelas Date: 01-1i-OS Client: TVA I Sequoyah Nuclear Plant - Non-treated 3 4 . 6 Analst . I 4 S -- - cetin- Parameter i f CN,`ROL pH (S.U.) 3 !1. 1jL%597 7 715.III .<{ w Z 7e DO (mg/L) 9-4 A.O) -wit ( 'S .-7 , - 3 Conductivity ,,, ~ ):/ (pnmhos/cm) Alkalinity (mg CaCOA/L) Hardness (mg CaCO)L) Temperahure (°C) 79((* .7 3A. Q 4.9lZl.l - ;. i. pH (S.U.) 7 . -7. ;; 7 ____ 10.98% DO (mg/L) .. 5 l ).3 8 IV' Conductivity__ 7o _ _ 13_ __ _ ____Tcmpenaturc ('C) 2q-1 Z{2 ^2 .l 7.~.'.Z Z5 . o w 2tIb,.52 pH (S.U.) (;imhoscm) Z3 5 *? S7; 1 .7. w - 170 7. sY 22% DO (mg/L) : 24. S .
~~.Temperature Conductivity~~
(°C) 7-4.7- '2, XZ.7 a5 _z5 0 x5. ZqA0 ss Temperature (°C) 7ZA .1 3z 14 &.S.2 243 25 .4.2. pH (S.U.) (jimhos/cm) 13 rS- 0 - ?_ -7' 43.9% DO (mglL) 2.-0 Z..12.q ;- ______ Conductivity (,umhoslcm) _ I83__ __ _ _ 2- __ Temperature (C) °Z(4
~~.9 >3 ZLq Fr i 25. I -Zs. ,~~
72% DO (mg/L) - C A 7 Conductivity 191 (Rxmhos/cmn) Temperature ('C) _ A3_ A l 2z-- 243- Z 2S. I 2ZG 2S pH (S.U.) . . nL l . 6G5 - 1 -t 7. 100% DO (mg/L) 4. y Conductivity ~,- (pimhos/cm) L°l I Alkalinity (mg CaCO9L) 50M Hardness (mg CaCIL) TR Chlorine (mg/L) ... '0Ix3 .fr _i Temperature (C) ZS.O -. .9. l 3 Z5 0___ 2S _ __ pH (S.U.) ________ ________2 Conductivity ~ (mg CaCO4f) ___ Hardness ' (mg CaCO3 /L) ___ __ VMS____ TR chlorine (mg/L) ~ Vtt~<0.10 V .4t ____ ___ ___ ___ ____Temperature rc) . 3. 1q 3V4.t qjj I 6. 73 2.1, 2.2. Initial I Final ' - Initial I- Final i - Initial Final - Initial i - Final
TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia Daily Chemical Analyses Client: TVA / SequoyAh Nuclear Plant, Outfall 101 - Non-treated Test dates: July 19-26, 2005 Project number: 1991 Reviewe bydI
~~- ~ ~~ ~Paraneter -~~
~~V 2V Concentration I I. nil (SU)~~
~~- DO (mrfL)~~
~~Cond-divlft tmn/m Control Alkalinity (mgtL CCOC) i t l Hardness (rnmgL CaCO1 ) 4-~~
.0-Ad-an emperaturelI l_____ L4., I.3. I L1.81 L:).L ZliD0 Z1U Iii. L3. I L'.D hO. L1. I L~u L3.A Zh.51 pit (SU) 7.81 7.77 7.751 7.90 7.90 7.79 -781 7.86 7.89 7.91 7.87 7.88 7.93 7.84 10.98% DO (mwL) 7.7 7.6 7.6 7.5 7 7.5 7.7 76 78 7.3 75 7.8 7.4 7.5 Conductiviq(pmhos/cm) 268 287 275 _ 296 270 _ _2 271 _26 Temperature (C) 24.9 25.0 25.1 25.3 24.7 24.8 24. 9 25.0 24.6 24.8 250. 24.7 25.2 24.7 p1 (SU)
_ 7.79 7.76 7.73 7.84 7.88 7.78 7.79 7.87 7.87 7.90 7.87 7.89 7.90 7.83 22% DO (mgtL) 7.7 7.6 76 7.5 7.6 7.6 7.5 78 7.8 7.4 7.4 7.8 7.8 8.0 Conductivity (umhos/cm) 255 274 260 236 256 l__2126 Temperature (C) 24.9 2501 25. 25.3 24.7 24 24.9 25 24.6 24.8 25.0 24.9 25.1 24.7 pil (SU) 7.74 7.68 7.81 7.85 7.75 785 7.83 7.89 7.84 7.88 7.86 7.82 43.9 iDO (mE/L) 7.8 76 7.6 7.4
7.6 76 7.4 7.7 79 7.4 7.3 79 7.7 8.1 Conductivity (pimhos/cm) 225 __ 241 228_32 224 226 230 Temperature (,C) 24.8 25.1 25.1 25.2 24.8 248 25.0 25.0 24.6 24.9 25.2 24.7 25.3 24.9 p1i (SU) 7.68 7.72 7.63 7.81 7.80 7.73 7.71 7.84 7.78 7.87 7.80 7.85 7.80 7.80 DO (mgIL) 7.8 7.6 7.6 7.4 7.5 7 7.5 7.7 8.0 7.4 7.4 8.1 7.8 7.7 Conductivity (umhoslcm) 183 19 184 191 183 _ 185 189 Termperature (C) 24.9 24.8 25.3 25.3 24.9 24.8 25.2 25.0 24.7 24.9 25.2 24. 25.2 24.8 pit (SU) 7.62 7.67 7.59 7.79 7.77 7.69 7.65 7.81 7.74 7.82 7.75 7.8 7.70 7.77 DO (mZ(L) 7.8 7.6. 7.6 7.5 7.6 7.6 7.9 7.9 7.5 7.3 8.1 7.8 7.7 Conductivitz (pmhostem) 143 _S 143 147 139 _ 13 143 100% Alkalinity (mg/L CsCO,) 50 50 50 alardness (mgtL CaCO) 56 62 56 Total Residual Chlorine (mg/L) <0.10. <0.10 Temperature CC) 25.1 25.1 25.51 25.31 24.9 24.9 25.21 24.8 24.8 24.71 25.1 24.81 25.21 24.
pI[ (SU) 7.64 7.641 7.60i 7.791 7.80 7.70 7.681 7.78 7.73 7.831 7.75 7.79 7.69 7.74 DO (me/L) 7.8 J 7.51 7.51 7A4 7.5 7.5 7.51 7.6 7.91 7.4 7.3 7.5 7.81 7.5 Conductivity (pmhostVm) 142 15 1 148 140 140 143 100% Intake Alkalinity (metL CaCO,) 52 52 51 Hardness (mg/L CaCO3) 70 6 56 Total Residual Chlorine (mg/L) <0.10 1 <0.10 Tempersture (IC) 25.0 25.11 24.91 25.31 24.7 24.91 25.01 25.1 24.7 24.91 25.11 25.01 25.2 24.7
Environmental Testing Solutions, Inc. Page 6 of 7 Species: Ceriodaphniadubia Date: cM- I9 -S5 Client: Sequovah Nuclear Plant - Non-treated Daily Chemistry. Analyst K; fT Concentration Parameter ;s. . 0O CONTROL pH (S.U.) - go i. fs DO (mg/L) Conductivity (g+/-mhos/cm) 2f Alkalinity (mg CaCO/1 L) b\ Hardness (mg CaCO "I4 Temperature (°C) 2LA .1 ZS . A. Z5- 24.
~~- a.t) pH (S.U.) ;_ j2, pZ .f-b 72.~~
10.98% DO (mWl) : z . Conductivity (uim~hoslcm ) __ _ _ __ _ __ _ _I__ _ __ _ __ _ _ Temperature (°C) 21.: z5 , 2S.1l 'S. pH (S.U.) SC 7.i7
~~9. -3 ?. S S'Y st?~~
22% DO (mg/l.) 2 v ; G Conductivity9I-Temperature (C) 24Ai -Z - 2 .1 1S 3. -2t4.-I pH (S.U.) . 4' 43.90/6 D (jg/L) s , .Y* ?. - Conductivity (gimhos/cm) __ Temperature (C) 7z.L.& Z-.1 2S. . 1 pH (S.U.) 9, r6 72% DO(mg/L) Gj 5 16 Conductivity (P rnh os./cm ) _ _ _ _ _ _ __ _ _ _ _ _ _ _ Temperature (°C) 'ZS . 5. - 2S.5 21 ZS.3 '.4. pH (S.U.) :Z .9. 2 .. ..2.S1 .. 100% DO (mglL) Conductivity (+/-Tnhostcm) 5 - Alkalinity (mg CaCOA) i i ny3 Hardness (mg CaCO3/L)l 10 b w" TR chlorine C) Ci <0 (mp-L) Temperature (0) Is-'. _______ -4 pH (S.U.) qeC ;.(0V-~1 T 100% Intake DO (mg/L) Conductivity OIznhoslcm) Alkalinity (mg CaCO/1L) 62 Hardness (mg CaCO]/L) 1 TR chlorine ./ (mziL) <0.1 - i _ __ _ _ Temperature ( 0C) .2~. .7Z5' InItial IIntilD IA Final Finil II InitiI Initial II B Flnal In1' I Initlal Initial Final
Environmental Testing Solutions. Inc. Page 7 of 7 Species: Ceriodaphniadubia Client: Seauovah Nuclear Plant - Non-treated Date: C1 OS Anals R7 1*s ?W_ El E Concen- Parameter 'W# tratio n _ _ _ _ _ _ _ _ _ _ _ _ _. CONTROL pH (S.U.) 7. 9 I 98 7 r-DO (mg/L) ij A Wn5. R 'i5 - 1 9-GsL.
~~. (jimhos/cm)~~
Ternperature (eC) Z .~n z; ESZA .- 9sZ5.' Zs . o 25.HZ E pH(S.U.) Conductivity w -{ 7; 2 O,_£ -;1 7 ; g ?q ;~
~~. Temperature (CC 4. j 2s.0 244... Zl.E 25.0O Z~~
~~2.5 .2 ZVl.1~~
_ l pH (S.U.) _7 ^. 7-J: ^.t -%7^ S -1 22% 10.98% DO(m DO (mg/L) .7L) Conductivity
.(Comhdos/cimi) _ as .a:70; . _ 1 Temperature (C) Z4l 't 2'.L 2'4.R 2S.0 - q pH (S.U.) Y.-3 7 Tepeatr (a 2 -14 -Z, - - -249 . -
Temperature (C) _s.O _44.o 2S. M.. 5 2q -1 .5.3 7-_4 .1 pH (S.U.) -1i M 1.i 9 -7 -7 7. n 72% DO (mg/L) 7. 4. 11 94 Y I. _ _- . *- W, - s} - .,, ,6. ,-_i I 11s.
. _L al l}'I-vI_-9\s Conductivity (umhos/cm) 'IV?
~~Temperature (C). z2S. Z5, 0 22 142 L -1 z~~
-5 7Z 72. T -S. -. 2. t pH (S.U.) . 14 -7l 1 -F 7 -J-100% DO (mg/L) ?- ./. j . -7. S Conductivity (mgCaCOl)
(mg CaCO 3fL) 5 TR Chlorine (mg/L) _ 1<.O.0 - Temperature(°C) 5.2.. s2 {+j .- .1 ZS. t 2q.S _S2.7 _L4 t pH (S.U.) c6 J '7 -7 .1 I 7. .1 . I- 2 .27 100% DO (mg/L) L < * -, 7. 3 * (Pmbos1cm) /4fg (4 In, t14 Alkalinity (mg CaCO3/L) guy."' Hardness M; (mg CaCO3 IL) IEI M ~M TR chlorine (mg/L) .T@. :J . l O;______ _Temperature(°C) 2S.0 2S 1 2 i1 . I. Z .. 2t'I Initial 11 Flnal I.W.1a Finsl I
~~a. ..~~
~~Inlttsl I Flnal~~
*-^r- *^z FIntial Final .
TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pinmephatespromelas Daily Chemical Analyses Client: TVA / Sequoyah Nuclear Plant, Outfall 101 -UV-Treated Test dates: July 19-26, 2005 Project number: 1991 Revltwedbyc Al Concentration P. ramet r Day 0 Di I Day 2Day 3 Day 4 Div 5 Day 6 Concentration __rmeer_ InitialD Final Initial I Final Initial Final Initial Final Initialf Final Initial iz Final Initial Final nil (SU) 7.82 7.54 7.75 7.79 7.91 766 7.80 7.74 7.87 7.60 7.87 7.72 7.86 76 DO (mdiL) 7.7 7.0 7.7 7.5 7.5 76 7.7 74 7.8 6.8 7.6 7.4 7. 7. Control Conductivity (pmhos/cm) 28 281 276 _ 3281 284 29E_ Temperature (C) 24.9 24.5 24.8 24.7 24.8 242 24.9 24.8 24.5 24.8 24.6 25.0 24 pit (SU) 7.81 7.53 7.74 7.77 7.91 7.62 7.79 7.71 7.88 7.61 7.87 7.69 7.88 76 10.98% DO (njL) 7.8 7.0 7.7 7.6 7.5 7.1 7.6 7.3 7.7 6.7 7.5 7.1 7.7 70
. Conductivity (umhostcm) 27; 290 _ 1 276 -_ 281 _ 271 278 280 Temperature (C) 25.1 24.4 24.8 24.5 24.8 242 25.1 24.2 24.8 24.3 24.9 24.6 25.1 241 pil (SU) 7.79 7.46 7.72 7.75 7.89 7.63 7.78 7.70 7.87 S 7.60 7.88 7.72 788 763 22 DDO(mJ/L) 7.8 6.8 7.7 7.6 7.5 7.2 76 7.3 7.8 66 7.6 7.2 7.7 69 Conductivity (pmhos/cm)
C 257 275 261 237 22 2564 mTeperature ("C) 25.2 24.4 249 24.6 247 243 250 24.2 24. 246 25.2 24.6 25.1 244 pt (SU) 7.75 7.43 7.70 7.8 758 7.75 7.71 7.8 753 7.86 7.68 7.85 758 DO (mg/L) 7.8 6.7 7. 7.6 7.6 7.3 7.7 7.3 7.7 64 7.6 7.3 7.7 67 4.9 Conductivity (urmhos/cm) 224 24i 232 259 227 231 235 Temperature ('C) 25.2 24.4 25.0 24.8 242 25.2 243 24.9 243 25.2 24.6 25.2 24.4 pI (SU) 7.71 7.46 7.66 7.70 7.84 759 7.72 7.70 780 7.55 7.82 7.71 7.83 7.62 DO (mg/L) 7.8 6.9 7.7 7.6 7.6 72 7.6 73 7 6.8 7.4 7.2 7.6 7.1 72 Conductivity (utmhos/cm) 185 20 188 228 186 185 190 Temperature(2C) 253 25.1 24.5 24.8 241 25.2 242 249 244 25.3 24.5 25.2 243 pitl (SU) 7.64 7541 75 7.65 7.80 7.54 7.66 7.69 7.75 73 7.76 7.67 7.77 753 DO (mg/L) 7. 69 7.7 7.6 7.6 7.2 7.6 74 7.7 67 7.5 7.2 7.7 69 100% Conductivity (pmhos/cm) 144 151 143 _18 141 142 139 Temperature (C) 25. 243 25.1 24.5 24.9 24.1 25. 242 25.01 24.4 25.3 24.7 25.3 242 pH (SU) 7.69 7.43 7.62 7.66 7.82 7.57 7.66 7.70 7.75 759 7.77 7.76 757 750 DO (mr/L) 7.8 7.0 7.7 7.1 7.7 7.4 7.7 75 7.8 6.9 7.7 7.4 76 69 100% I ntdke nductivity (pmhosfcm) 14-4 151 t 141 139 9 14 142 _ Ttemperature(C) 25.2 24.2 24.9 24.4 24.7 24.1 25.1 24.2 24.9 244 25.2 24.6 252 244
> +i_ -sf Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephalespromelas Date: __ 1__ -_ _° Client: TVA I Sequoyah Nuclear Plant - UV-treated Daily Chemistry: ._ _ _ _ _ _ _ _ Analyst Y 4 Concentration Parameter d 9 :
CONTROL pH (S.U.) DO(mg/L) . )5TA .O ;. . Conductivity (gmuhas/cm) Temperature (c) ZI Si .5 '- *82'4L .- pH (S.U.) ? ;.?5 10.98% DO (mg/L) l_8._6r Conductivity (pim hos/cm)__ _ _ _ __ _ _ _ __ _ _ _ _ Temperature (QC) z5 I 2 . T 2A.5 2L. 2A. Z pHl (S.U.) .;,qC e6 -;7 22'h DO (mg/L) _G8___ Conductivity (gmnhos/cm) _____ _ TemperatureCC) (Q .2 2.4. I.I - b L'1 1.3 pH (S.U.) ___43______ 9 & 9 5 43.9% DO (mg/L) :;7. .3 Conductivity (pimhosicm) / _ _ ___ _._i Temperature (C) -Zs.L-3 -*q tA Ze 2 1 . ? 2I P'H (S.U.) .Y . ff ?, I .& a q.s y 72% DO (mg/L) - Conductivity (izmhos/cm) _ _ _ _ _ _ _ _ _/43 Temperature(C) ( ZS-3 2.2- 3 I . _- I Z.'i7 pH (S.U.) I__ __. __ 100% DO (mg/'L) Conductivity, Temperature___(_ 2.S.3 2'I. 25.1 24.1z4S~ -q.i pH (S.U.) -; 1 . 100% Intake DO (mg/L) .O ?j I> Conductivity I (pmhporusrcm) S.. 2 /1/ ITemperature(°C I2'S.' 2- 7- Z. zq. r ri. V- l-1 Initial a Final Initlal Final Initial Final
~~- i -L .aa~~
Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page6of'6 Species: Pimephaiespromelas Date: O-l-i C5 Client: TVA I Sequoyah Nuclear Plant - UV-treated Anal-At Concen- Parameter l tratloii __- "-,________W M CONTROL pH(S.U.) -1. TZ -7.6& 0 1 W, Q. 2- I . R(. DO (mglL) l C ConductIvity (pmhos/cm) ______4__ Temperature(C) 3*%
. Z9 4 Zt. _____ 2zq lL2'4.(. 25.0 3 pH (S.U.) . . C 10.98% DO (mglL)
Conductivity 2 i _ _ _ _ _ ___ Temperature (°C) s. z.& 22.L. 213.9 b Z5o
~~--. 1. \-.~~
pH(S.U.) .. o 0L -. i __1__ 22% DO (mg/L) . . 6 +i: *-- Conductivity da5l (pglmho~s/cimi) C t S ) Temperature (°C) .O 2. . 2. 224 ( ' S. Z . (a 2S \ pH (S.U.) __ __ _ _ . I 7.__ 43.9% DO (mg/L) Conuctivi~ty __ ___ Temperature CC) 2s* 75.Z. .a. pH (S.U.) = . 2- 0 i7. 7 -SO 7. X5 72% DO (mgL) Conductivity 2 I *flr (pmhos/cm) ~ ~ __ __ Temperature (°C) ZS.L . 2. 7A q' 241-1 25-3 214. S ZS*2_. zc4 3 pH (S.U.) - 9 c; 71. 6 i .-7 r i t 100% DO(mg/L) 00; ConductivitY i9S _ 19 I _ Temperature (C) -s. 2S 2A.2 Zs .0 37 q 7.3 pH (S.U.) L7.7 O 7.75 7 100% DO (mglL) 5 .G - / Intake 1- L/C Conductivity /3. s: g.i (_mhos/cm) . :_L_ . . . _. ITemperature 17C) ... 3.L2q.9 q1 25.2 2. 0 ZS.2~ Initial Final Initial Final TnItlal Final I Initial . - Final
Page Page I Of 29
~~,3.3 Environmental TestinZ Soluttions, Inc.~~
Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO39L Analyst l II t I ZI Date analyzed toq-1-45 I Titrate samples to pH = 4.50 S.U. s.. Titrant normalt and multilier determination: I, pH of Delonized water Titrant rererence Normality check standard Begin ml End ml Total ml Normality (N) of H:S0 4 (5 ml ?a CO3 x 0.05)/E
=0.25/E pH Factor or Multiplier (iVx 50000)1 100 nmlsample - IVX500 -4.5 S.U. number number (E) (accept2ble range - 0.013 - 0.022)
I -6 q IIARI-% 1rj~j:- o.1 1,71 1,2. 0. ( Pi 5 Io ' Lab oratorycontrol standard: I. Reference ITrue value Sample 7 lAlkaliity (MV)' % RS = *1V/ITV sl10 standard number (TV) volume Begin End Total Multiplier (Ing C2CO-/L) (acceptable range (tng CaCO3t1) (ml) ml .ml ml - 90 to 110%) I. VAS2s100 100
~~-II6 12.o '2l j1 1b I 102Z7.~~
~~Duplicatesam le precision: I Sample number Sample ID Sample volume (ml) 2Zl Begin ml j5.0 End ml~~
~~.5).~~
~~Total ml Multiplier Alkalinity (mg CaCO)IL)~~
~~%RPD I(S - D)I[(S+DY211 x 100 (acceptable range - t 10%)~~
~~I ON- oS5 SsJ Duplicate (h 10 _ -tO , 2.9.0 7h.2 3,2Z~~
~~d. _.~~
~~Io. S. D 3~~
~~.. . .. . .c 2 . D .~2~~~
I Mkatrix spike recovery Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO;dL) I SSASE (mg CaCO2/L) 5-0 (ml) Ica ml 25.0 ml 3.{ 1 8-0 ml 1.4 03 Samplealkalinity(B) lryeasured spike value (MV) % R MIV /SV 100 (mE CaCOA.l) NIV - A - B (acceptable range l _ l (mg CaCOVL1) -75 to 125N.)
~~;33 6. 1001.~~
Sample measurements: Sapevolume Begin End Total Alkalinity Samplenumber SamplelD (ml) ml ml ml Multiplier (mgCaCO,/L) 0 -4I mOS A Ml4S t-b j..oq 13. I , to04O 0-4-IB-09~~* 4q r 1 , l4o I 0' 6Ca+ -oA _ I 14*2- 18.1 I -_1 1 1 0-1-3 -3OS 6 Jq Wm____ , ~z 14. l 5cc txre- _2l Z l ?A42, 1 _11_.4ll _ _ __ _ & A X' ty c b Z .1 a1. S l4 . t S. z _ _ _ _ 1l mls cr . t mo 1 j ;.- 1t2.o 1 I.1 i ii_ _ _ __ _ _ _ _ __ _ _ 1. z3~ s . o.' t1 __ i-b Reviewed by: ir Dateereviewed: i -- -I~o;o
Page (.%) Environmental Testing Solutions. Inc. Page 2_ of 3 Alkalinity (EPA Mlethod 310.1) Matrix: Water, MDL = 1.0 mg CaCO/L Analyst Date analyzed 0L - 1-o Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH Normality (jN) of H2SO pH Factor or Multiplier Deionized Titrant check Begin End Tota-iC x 0.05)IE = (INX 50000) 100 ml sample water reference standnrd ml ml ml = .81 _ Vx S00
~~-4.5 S.U. number number . (E) (acceptable range - 0.018 - 0.022) 4 Laboratorycontrolstandard.~~
~~Reference standard number True value cm Sample volume Begin End I Total Multiplier Alkalinity (MV) (mg CaCO31L)~~
~~% RS -lV /TVx 100 (acceptIble nnge (mg CaCO/L) (ml) ml ml ml - 90 to 110%)~~
lJ As J;Gsb 100 100 13.e8 z3m q-6 JO.q bo ( 02 1 Dup1licate sa nle precision:- - - -. s Sample Alkalinity %RPD - [ Sample Sample ID volume Begin End Total Multiplier (mg CaCOA.L) J(S - D) /[(S+Dy211 I100 number C9-ll-os (ml) ml ml ml (acceptable range -
~~10%)~~
~~0 I .f-p [ dh6-Duplicate~~
~~, 23.Lp 31.51.co i3c, O.. 1?."~~
Io.L. S; D 2,o 2 .. Mfatrixv spike recover :_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Reference Spike value Sample '~Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (fig CaCO3IL)
. (mg CaCOjL) (ml) ml nml niml I/8ssagb l too 50 0.0 l 1.t4 n4AJ L24 lO.l . 3(io Sample alkalinity (B) Measured spike value (NM) % R - MV / SV x 100 (mg CaCOjlL) MV - A - B (acceptable range l (mg CaCOj/L) 75 to 125%)
Z(0c tOcj1 I 100'1. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mE CaCOV/L) 0603Xo 17-1 Oaaja I Q 1qwq 12S4 15 51CO) 10.4 I I lOs 1 l _ l 22.? 1 1 l~ l l (oZO bSlYi lIoe'. _ _ _ _ _ _ _ ___3i 3 j.j I L5A(o b6b-4l2.03 C.Lcew L I loc) &1iIq 7(-t___ ml r4)lO. C _O5___ ___ r c.OI 3 l z - ____ '12.1 S l . lO l l q _ _a _ _._5 .3 JI l il i .. L l I l IQ5O91Z£ F~x~o I_ - I I ___ I -,%I. I O539 1 q.09 18~ 2. }17.1 2G.~2Z1~ 1_ -,. 1( Reviewed by: *Da\ Date reviewed: reew Ie Oz I- 1 Q1t3
Page () I Environmental Testing Solutions, Inc. Page 3 ofI I Alkalinity (EPA Method 310.1) Matrix: Water, MDL 1.0 = mg CaCO 3/L Analyst KEj)y-O3-Date analyzed Titrate samples to pH = 4.50 S.U. Titrantnormalty and multiper determination: Normality (N) of H.SO4 pH Factor or Multiplier Deionized Titrant check Begii *(Nxml NazCO 3 x .05YE E= 50000)1100 0 ml sample standard ml 0 ml mlZ water reference range 0.01 0.022) number number (E) (2cccptable
~~- S.U.~~
4.5 Laboratory control standard: Reference True value Sample Alkalinity(V) (M %RS-vITV x 100 standard number (Tc) volume Begin End Total Multiplier (mg C2COjL) (acceptable range (mg CaCOV ) (ml) ml ml ml -90to 110%) j.9 - 10.QtI) O9 I 14-S :?5t . 100 100 1&0S 14@(OL9 D licatepsam lc recision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) ((S- D)l[(S+D)1211 x 100 number (ml) ml ml ml (acceptable range- 10%) oToII . 10 04 D,
s
~~.,\ oo.1 to.-( L Duplicate 4 p, IO.q 10 .q D .2-Afatric spike recovery:~~
[ Reference Spike value Sample volume Begin End Total Multiplier Spike alkalinity (A) (mtgCaCOjL) standard number (SV) (mgCaCO)L)l (ml) ml ml ml l
~~,) Aas 1 I00 6A 5.(-o .2 IO-%q 51 l Sample alkalinity (B)~~
~~(mg CaCO/L)3 Measured spike value(MIV) MhV - A - B (Mg C2COlL)~~
~~% R IV - ISVx1OO (acceptable range 75 to 125%)~~
I
~~.4.2 '4q15 CRqhO,?.~~
Samplemeasurements-Sample volume Begin End Total Alkalinity Sample number SampleID (ml) ml I ml ml Multiplier (me CaCOL) iI l.o 82b *Zl2.1 O l)q 0 3 3 l06?)O- ll l 2 153 CM 9 i < }z . 5 63,0 O bs>01qn3DT svs.OL S 3 2 3.3 .3 0 1-loVlOY+.o.W I l_ &.I 14q.(O 66 l l Be I3 .32.c,. z. 10. rQ ,r _____ IPeca. tl- ___ _ 132e4 134.S DacPPwd lo _____J zO Reviewed by: De rev Datereviewed: I 0&-07-C6-]
~~.i 1-0 P e 1 *- - _~~
Environmental Testing Solutions, Inc. I.,-~ .. Alkalinity I;.I. Analyst (EPA Method 310.1) Matrix: Water, UDL = 1.0 mg CaCO/IL Date analyzed 0 Titrate samples to pH = 4.50 S.U. Titrantnormality and multiplierdetermination: pH of Normality Normality (N)of H 1S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml NasCOj x 0.05)/E (IVx 50000)1 100 ml sample water reference standard ml ml ml 0.25/E -NsX500 4.5 S.U. number number ( (acceptable ranage - 0.0t8 - 0.0'2) t/;7 1A1918Y IAJR15'7 O.O 1*7. 12 , 4O./°le 7. 9 JJ9 Laboratorycontrol standard: Reference True value Sample Alkalinity (MV) % RS-MhV/TVs 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable ranne (me CSCOVLI (ml) ml ml ml . 9*goto 110%) jg1s 00 1* l / 6 2.7 i. 999 .9 .77 0 . D uplicate sa mple precision:__ _ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Sample - Alkalinity %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) ((S - D)Il(S+DY21I x100 number . (ml) ml ml ml (acceptable rnnge- *10%) 07.18.05 MUs b 1/LO /,O . 48.7 -O elj- ; Duplicate , a 3 o j D. - Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total MultiplIer (m"CaCOVL) (mg CaCOV/L) (ml) ml ml ml
jjV5S Q5 e-el 13-0 1II .. glo /070 ffq. /IoI Samplealkalinlty (B) % R - MV I SV x 100 (mg CaCO,/L) I Mcasured spike V- A - B value (MV)
(2cceptable range I (mtg CaCO,/L) 75 to 125%) Sample measurements: i Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOIL)0 07.I1.305 Mh/ sW o0A /O o.o C.o I.0 q 3 5 lo5<nzm. O -ro-.3 l Io lG.O l/o.7 40l § l lOSI2 1 l QI0 4.2 l Osc Ok -lron l job 115-.152 4.7
.77 lo4ON Iorj f lot t oo -.20-al< J. l* 1. 1 rjai5O\ 5e1.dtJ;I IO (00) O1a45.;13 51.o I5 C.Sfl= . /06 . .3o.3 . I .*0 O ZiZa.c C) q (0 to0 .. s3 50 c)SCI-LO. q sc5J IN4 /coo 4. 1f.9 _____
Reviewed by: l Date e reviewed: e d I Ok02 0s I
I' l'
~~.. 4 ........~~
~~Page o a Environmental Testina Solutions. Inc. Page .. Q of - Alkalinity~~
~~,,i(EPA Method 310.1)~~
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L i_ l Date analyzed 01.j714 0 Titrate samples to pH = 4.50 S.U. Titrantnormality and multiplier determination: pH of Normality Normality (N) of H:SO, pH Factor or imultiplier Delon, Titrant check Begin End Total (5 ml NalCO% x 0.05)/E - (.Vx 50000)1 100 ml sample water r~e l -4Ukred- -- ml ml B:-Ns 500
~~- 4.5 S.U. number number (E) (acceptable range --0.01.-OI0 Laboratorycontrolstandard:~~
Reference True value Sample Alkalinity (MV) % RS - Mv I TV x 100 standard number (TV) volume Begin End Total Niultiplier (mg CaCOyL) (acceptable range (Mg C2COmL) (ml) ml ml ml _ 90 to 110%) IM5sa s 100 100 0.0 o o10. 0 /o.O9 I/O Duplicate sam le precision: Sample Alkalinity %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOA) [(S - D)Jl(S+D)1211 x 100 number . (ml) ml ml ml (acceptable ranve - 10%) OSa-M.z 5ek /M, 3 /° O6 .0 S.; 5.a . 55 ;; . . 4, Duplicate 7 s .a0.3 s 1 I / D a M atrixspike recovi y _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Reference Spike value Sample Spike Alkalinity (A) standard number (SV-) lvolume lBegin lEnd lTotal lMAultiplier l(mg CaCOVL) (mg C2COVL) l(ml) l-ml lml lmll JA f8 /067 15a2.53 /o° I
~~'9 /1, rSample akalinity (B)~~
~~(mg CaCOA1L) Mleasured spike value (NIV)~~
~~. IV - A - B l R MIV I SVx 100 (acceptable range (mg CaCO/tL) = 75 to 125%)~~
l50 50 /OD i Sample measurements: Sample volume Begin End Total AlkalinIty Sample number Sample ID (ml) ml ml ml Mlultiplier (mg CaCOJL) log38alqozm C . / - Im:C 100 ;S.3 31.1 S.S q1 s 7 IDSO':3ZD3I r Aopowl ^°e 600 s3- 1i. 8 -.1 ________ 50 3. 5.o l.a0 - 1301 I~~w - R by: 4D D1 9 DaIe 9 reviewed: I I ~~ . . . . . . . . . -1 Reviewed by: LI I1 Date reviewed: I ~- 02 -S -1
'-'v': : Page _ _ _ I.. Environmental TestingSolutions, Inc. Page I of . . Total Hardness I (EPA Method 130.2) Matrix: Water, MDL 1.0 mg CaCO3/L Analyst 1 Date analyzed [ 1j43O ] strantnormality and multiolierdetermination: I Titrant reference Normality check standard Begin ml End ml Total ml Normality (A) of EDTA
- 0.2OE pH Factor or Multiplier (N x 50000)150 ml sample number number (E) (acceptable range - 0.01S *0.022) =N x 1000 INA10Z )pjq 9a3 D*O *1 0 L7 0. 02C c 20A0 Laboratorycontrol standard:
Reference True value Sample Hardness (NLIV) % RS - MV/TVx 100 standard number cV) volume Begin End Total Multiplier (mg CaCO 13L) (acceptable range (mg C*CO/L) (ml) ml ml ml - 90 to 110%) 40 S.q O (, 9 -S), Duplicatesam ple precision:* *_ Sample Hardness %RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) ((S - D)Il(S+DY21 x 100 number (ml) ml ml ml (acceptable range - 10%) 0-2i %-o- _Ssw (tt 2. t 7-0,(o_ S y4n 7 l Duplicate _ 13-f - Z. I .w D0 _ l Matrixspike recovery: Reference Spike value Sample Spike hardness (A) standard number (SV) volume lBeginl End Total Multiplier (mg CaCO/IL) C&COA) *(mg(ml) ml ml ml A39(4-0 C b Jji ,Ji i 4'o zo.t.-o Sample hardness (B) Measured spike value (MV) % R-MVISVX 100 (mg CaCO,/L) MV - A - B I (acceptable range (mtg CaCO/L) -75 to 125%) i£43 i ?.59, C* Sample measurements:. Sample volume Begin End Total Hardness Sample number Sample ID (ml ml ml ml Multiplier (mgCaCO/t) Tl r lIJ (hould be - 0 mcnCaCO,/L) Z°_ Do 0 .. _ _r _ _ _ _ D04 '15-S A ___-4 r3, -142cf I ZJ*1 21S-4 ' _ _ l 0? SO.? t_( OEZO A 1-sq D.? ___ _ r 1 05b6?I't1'5 L 2 ___ 1__ _ 20.0 04 _____ 8.Z 3'L) Note: If >l50 of titrant 'SUs Supple must be diluted. Reviewed by: I A l Date reviewed l CZ0-O 1. w
Page ; 9 Environmental Testing Solutions, Inc. Page 2: of .. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst 1 Date analyzed Titrant normality and multiplier determination: f ;*..-.
oro-aty1-b reference standard EI -Regin ml End Total Normality (A)of EDTA pH Factor or Multiplier ml mI (' x 50000)! 50 ml sample number number = E) (acceptabie rnge -0.018. --. -1 h' 1000 Laboratory'controlstandard: _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
Reference True value Sample Hardness (MV) *. RS MV TV s 100 standard number (TV) volume Begin End Total MIultiplIer (mg CaCO*JL) (acceptable range (mg CaCO/L) (m ml ml ml _ 90 to 110%) 1I5 $ 40 50 q27.s 2 .I ZO.(v 3 1o'59 Duplicatesam ple precision: Sample Sample ID Sample volume Begin End Total T Multiplier Hardness (mg CaCO,/L)
~~%RPD~~
((S - D)IL(S+DY211 s 100 number (ml) ml ml ml (acceptable range - 10%) Cb 1 't A09 !O.J 3 _~ - 5 20 S B. _ Duplicate __D5o 22. Z 3 2 D z. Matrixspike recovery: Reference Spike value Sample l Spike hardness (A) standard number (SM volume Begin End Total l hultiplier (mg CaCO31)} (mg CaCO3/L) (ml) tn ml ml fi~s2 65l 5L) l4'R21 26-3 1 l SD: tl20-to lq4 Sample hardness (B) Measured spike value (V)l % R NIV I SV x 100 (mg CaCO/L) MV - A - B I (acceptable range I(mg CaCOAL) 75 to 125%) lIb.I 102 ?. Sample measurements: - - Sample volume Begin End Total Hardness Sample number -Sample ED (ml) ml ml m2l Multiplier (mg CaCOA) l l ~~~(should be mg CaCOytL)l _ lOt:5o0 12.0-t Mmm CL P . Il2S.3 2(05 l .Z. l D. (a ? OSb-3aI4.o5, I Z t Z.5 Z?.7 1 I7 l Z 050o41S.02- 3 4 1,5Zl. Z--? 2 l l6 Q I0~l I'
~~> la ° ltn1 1I 1 I 33 41qZl2 I~~
l l lI Y ltSb3ato.ICo 11 1 I 4 4-'- a.q 4-* I I 1- iI1
~~, I I I~~
~~_ _ I I I~~
- - -7 4-1 JI Note: if > I Smi of titanlt is used, sample must be diluted. Reviewed by: I a I Date reviewedI Oa O2_cOs . 1 .
Page 3O Environmental Testing Solutions. Inc. Page / of 42 Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCOQL I. Analyst Date analyzed lL i.i -s I.5 T&iant normality and multiplierdetermination: Titrant Normality check Begin End Total Normality (A)of EDTA pH Factor or Multiplier I.- reference number I R 16? 1,A g (A.3 standard number 0.o ml I ml
/o.o ml (E) /0- o -0.2/E (acceptable range -0.018 -0.022)
~~0. o L0 (NVx 50000)l 50 ml sample~~
~~- iVs 1000 Laboarawy control standard:-~~
Reference True value Sample Hardness (MV) % RS - MV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CICO,/L) (acceptable range (mg CACOL) (mD ml ml ml .90to.110%) siss a-i 40 50J 10JJ0 . 2-1 c2D 4a 105 Duplicate sample precision: _ _ Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg C&COIL) {(S - D) 1(S+DY0111 %100 number (ml) ml ml ml (acceptable range - 10%) D5upliae ° iO 4.1 41 s L Duplicate [ 0.1~ 0 o D s7
Matrix spike recoveru Reference Spike value SampleI Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO/L)
(mg CaCOAL) (MD ml ml I ml 10t5 aa-7 40 30 16.a C1 ,.31 6-/I IQ0 /9,2-Sample hardness (B) Measured spike value (MV) % R - MV I SV s 100
* (mg CsCO3tL) MV - A - B (acceptable range I I (mg CaCO 3/L) 75 to 125%)
oaI40 1 lOb e/o Sample measurements:. Sample volume Begin End Total Hardness Sample number Sample ID (mD ml ml ml _Multiplier (mgCaCOA) l7_(shouldbe_-_mC_ CO_L)l 50 0.0 0.0
~~°o. 0.0 N°~~
________ va.o5 5o .2; .3 4., aO ? I _I_ CR. m R 1 0 l3 - (e q 0 l 05S ti2. 01 l6~-1Wv 1" 1 ,sj 0.613-5.412.2 1S L, laS23.-CA ;G 41 3 1 5° O 35.0 37.3 1.1I ______._ 5 41 ID1 50 237.31*04lR
&SUIZOO-3 0! N 1 1- 5° 1 0.° ;T4J TIT 1 IYSC12. O -.1;) 3 2 50 0.o- 2a. 1 s 56 5wr 60 a- __ 1 1 7o Note: If >1Sml of titrant is used, sample must be diluted. Revie wed by:
[ T f l I Datereviewed a rI0 08-oz-s
Page 3 Environmental Testing Solutions. Inc. Page - of Z Total Hardness (EPA Method 130.2) Matrix: Water, MDL =1.0 mg CaCOA Analyst [I rQ I Date analyzed OII aI.o5 I Titrantnormality and multiplierdetermination: Titrant reference Normality check standard Begin ml End ml Total ml I Normality (N) of EDTA 0.21E pH Factor or Multiplier (N x 50000Y 50 ml sample number I numb -_er _ (E) (acceptable range - 0.018 - 0.022) -N I 1000 II Laboratorycontrol standard: Reference True value Sample Hardness (NMV) % RS = MIV /IV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3L) (acceptable range (mg C.COJ/L) (mD ml ml ml _ 90to 110%)
~~/W1S Z7a7 aS 40 50 94.o ao 4 Du licate sam lc precision:~~
Sample Hardness %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L) J(S - D)/[(S+Dy211 a100 number (ml) ml ml ml __ (acceptable range - 106%) 6f 0 8)-7 C051-z.tt 50N
~~. Duplicate I l 111.5 141 3 i.36.~~
D 4 A 'I/O M atrix spike recov e y__ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Reference Spike value Sample Spike hardness (A) standard number (SV) volume mlegn d Total lultiplier (mg CaCO/L) (mg CaCOOL) I(ml I[ j ml IMSsa r7 40 I so -2 0 1 Io i Sample ardness(B) lMealsuredspike value (MV) l %R -MIV/SVX 100 (mgC4COA; I - A -B (acCeptable range I (lmg CaCO/IL) - 75 to 125%) O6 46 0 &/a Samole measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOL) l l ~~Blank I _ l l ~~~~~(silo ld k - 0e~yC9L IoSbt.o31Q otjql'2-z t C. A mr X¢. 1- .( 5 _Q I /.7 7 I II r ___Szg 50G 7_ o4 (' 1 4i3 4*O ARl. l r. _ _ _ _ _-_ __._ _ 7.;a-Ro5l Mqs BH.O A't 5o 124-) 12S. 4-'1 11 7.I. 15 1Hfl 1 1 I_ 16 I Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: l WI Date reviewed IO--Os I
rnvirnnmental Testing-Solutioos, Inc. - 4' 3
~~-.. 2.~~
Page 43 Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L E Meter Accurnet Model AR25 pH/Ion Meter Analyst &P I Iodide reagent: I ItJ I (.Oq 1 Date analyzed Acid reagent: IIN R I S15 I Calibration: I . - .o .1. -. l 0.10mz/L 1.00 mg I Reference standard number l SS wsSa76 I Nhote: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorycontrolstandard: Reference standard True value MTV) Measured value (MV) %RS-MV/TVx 100
~~. number I (MtfL) (mg/L) (aceeptable range -90 to llO.)~~
I ssy 0 .50 o.4 5- Oil, Li Duplicatesample precision: _ __ __.
* ' Sample Sample ED Sample charncterlstlcs Residual chlorine %RPD - {(S - D) /I(S+D)Yl) x ID00 number . * (mL) (acceptable range - t 10%) ;- w
~~mtu ioi fOcP , CLQOe.- a S Zonoeyoe ______s_0_____~~
~~0*-"..~~
~~.Duplicate : . $ D zos3 j Sample measurements:~~
uSample Sample ID Sample characteristics Residual chlorine number . __(gL
.4. . ., .Blank (shouldbe<0.1omg/L) - , 4--I @ cSt.aoz us- 5- itJm o.t C1lQ.t (SnhJis A~o.oxo(z ;u_ _.- - _,'00062'?
~~2"- 4 a.~~
~~,.{X _ _ ___.k _j4_q _0D S..~~
~~aA1 S.,- -~~
~~1 rs Zr Note: All samples were analyzed in cx:es s of EPA recommended holding time (15 minutes) unless otherwise noted. -a~~
~~~. Labortory control standard:~~
2;rt Reference standard True value2(TV) Measured value (%V) l % RS - IV /TV x 100 I _ number_ (mg/L) (mg]L) (acceptable ranec - 90 to 110%) S -- - b~at . 0.50 0.501 :0 2. I1 Reviewed by l A Date reviewed rn i:
~~-.- W.5_~~
Environmental Testinte Solutions. Inc. Page _ _ _ Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mglL Meter: Accutnet Model AR2S pH/Ion Meter 7? i Analyst I v 4 ( I Iodide reagent: l7 Date analyzed 0 2 1*S I Acid reagent: f//A/le! l Calibration: I :-: . v .. - . -.: ; . ..- 0.10 meiL 1.00 me/L Reference standard number I/1t9:7 r 2 c 1 Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorvcontrolstandard: Reference standard True value CM Measured value (MV) % RS .UV M / TV x 100 number (mfgL) (mgaL) (acceptable range - 90 to 110%) A l '5 ~0.50 i O. 3-0 Duplicatesample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD - {(S - D) /I(S+D)12l)x 100 number ._ _ _ _ _mg/L) (acceptable range
~~10%)~~
t.
~~-Duplicate .1< s . D~~
~~Sample measurements:~~
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
Blank (shouldbc 0 0 m trmzo.oul -rVA

ti - t fr OeadO. /
Note: All samples were analyzed in cessot0EPA recommended holding timc (15 minutes) unless otherwise noted. Laboratorycontrolstandard: Reference standard True value (TV) Measured value (NMV) % RS - ,IV/ TV x 100 number (mgL) (mg/L) (acceptable range - 90 to 110%) 5z O 0.50 °0. oo.7 Reviewed by I -_ Date reviewed I n'z;- 3
* [Po = 4 ...q. .
~~.:Environmental Testing Solutions, Inc.~~
~~Page 74 C Page I of l_ Total Residual Chlorine~~
* (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst k1bA I lodide reagent: I /1.JR 176 Date analyzed 07.a13 .OS Acid reagent: I /mg 16 Calibration: I , ' "' ' . : l O.0tnmL l 1.00 m7L l l Reference standard number I lAssa I nu sa I Note: Forsamples with a residual chlorine of > 1.0 mglL, the calibration range rmust be adjus~ed to bracket the chlorine levels of the samples. Laboratorycontrol standard: Reference staudard True value (TV) Measured value (MV) % RS - IMlVITV x 100 number (mgrL) (mg/L) (acceptable range - 90 to 110%) 0.50 0,504 . lOlO el Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD ((S - D) I!(S+D)/21) x 100 number . ( (acceptable range - s 10%)
)Q42-.0) ru-R ArS&N- &0i Au°p"41,1ts S 0, 00 :Al-,'8 o7 - s ;*_ -Duplicate D o.OoIg -
Samplemeasueaets: Sample Sample ID Sample characteristics Residual chlorine number (mglL) 7 Blank (shouldbe <0.10 mgl f. ' . O. 00O4*3 C Z. 'TU-i Q aIT " l ) a4 e., &,4l'&I( 0. 00o M 7.a
~~?2 Q. C jtnj~CLn S.- o e iO dr 'c, J n Jo , O.' ooo >~~
6!5b-2S-02 Mt. 0l~ 1 P.98h JaA Co1 no 0 $4110 /f1effme_ Zo. 000a to OSb2-SG > u e f ngo,1 06a.4,' e ffi5 O . 00 316 Ntote: All samples were analyzed in excess ofrEPA recomntendcd holding time (15 minutes) unless otherwise noted. Measured value (MV) % RS -NIV /ITVs 100 (m&(L)I ceptable range - 90 to I 10%) I O(. R /0 Reviewed by Date reviewed r i- a-o
Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix D Reference Toxicant Test and Control Chart
Environmental Testing Solutions, Inc. Pimephalespromelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 I I I , i I I A I I I I I I , , I* I USEPA ControlLimits (I 2 StandardDeviations) 0.8 F 0.7 0.6 0.5 I I I I I I I I . . . . 1 I I I I I I, 1.2 I I I I I I I I I I I i I I I I i I I l USEPA Warning and ControlLimits (75 1h and 9 0jh Percentile Cls) I O O&O ,.... t N-V 0.8 _---.. . * .. .............-----*:-.: ........ U 0.6 O* C4 10 t-no. V.W I Laboratory Warningand ControlLimits (leh and 25'h Percentile CVs) 0.8 0.7 0.6 0.5 ,....................................... . I. . . . . .. . . . . . . . . .. . . . .. .. . .. . . . . . . . . . . . . . . . . . . . . . .. .. . INAI Test date
~~----- -ay IC2. = 25% inhibition concentration. Am estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.~~
~~--.- Central Tendency (mean IC.5)~~
~~--Warning Limits (mean IC.5 i SA 1o or SA.75) *Control Limits (mean IC2.s + SA_25X SA.90, or 2 Standard Deviations)~~
Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart ror Pimephalespromelas using Moderately Hard Synthetic Water State nd USEPA LAbartery laberatery USEPA USEPA Tntnum ber Tea date edaylqC Cr S Central lUmit SA. Wanfn Lbntt SUs Centra Ltmltb SA1 WarnIng Limrts SA. Confrol imits CV (DLlMD (Vl XCI) CT4-2S CT+2S CT-S9AI CT+BAI. CTr-ASA CT+S.Ug CT - SMA CT + SAYS CT -SAWe CT + Sx, 1 11-30-04 0.54 2 12-07.04 0.64 0.59 0.07 0.45 0.73 0.07 032 0.66 0.12 0.47 0.71 0.22 0.37 0.31 0.27 0.32 0.86 0.12 3 01-11-OS 0.63 0.60 0.05 0.50 0.71 0.07 033 0.67 0.13 0.48 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.09 4 02-4S-05 0.62 0.61 0.04 0.52 0.69 0.07 0.53 0.68 0.13 0.48 0.73 0.23 0.33 0.84 0.27 033 0.Q8 0.07 5 02-12-05 0.62 0.6t 0.04 0.53 0.69 0.07 0.54 0.63 0.13 0.43 0.74 0.23 0.38 0.84 0.27 0.33 0.88 0.06 6 03-01-05 0.61 0.61 0.03 0.54 0.68 0.07 0.54 0.63 0.13 0.48 0.74 0.23 0.33 0.84 0.27 034 0.88 0.06 7 03-01-05 0.61 0.61 0.03 0.55 0.67 0.07 0.54 0.68 0.13 0.4t 0.74 0.23 0.38 0.84 0.27 033 0.88 0.05 8 03-0S-05 0.69 0.62 0.04 034. 0.70 0.07 0.54 0.69 0.13 0.49 0.75 0.24 0.33 0.35 0.28 0.34 . 090 0.07 9 03-15-05 0.62 0.62 0.04 034 0.70 0.07 0.54 0.69 0.13 0.49 0.75 0.24 0.38 0.85 0.28 0.34 0.90 0.06 10 03-29-05 0.67 0.62 0.04 0.55 0.70 0.07 055 0.70 0.13 0.49 0.75 0.24 039 .86 0.28 0.34 0.90 0.06 11 04-12-05 0.61 0.62 0.04 035 0.70 0.07 055 0.70 0.13 0.49 0.75 0.24 039 0.56 0.28 0.34 0.90 0.06 12 04-26-05 0.52 0.61 0.05 0.52 0.71 0.07 054 0.69 0.13 0.49 0.74 0.23 0.38 0.85 0.28 0.34 0.89 0.07 13 04-26-05 0.58 0.61 0.04 032 0.70 0.07 054 0.69 0.13 0.48 0.74 0.23 0.38 0.84 0.28 0.34 0.89 0.07 14 05-03-0S 0.61 0.61 0.04 033 0.70 0.07 0.54 0.69 0.13 0.48 0.74 0.23 038 0.84 0.28 0.34 0.89 0.07 15 05-10-0S 0.58 0.61 0.04 053 0.69 0.07 0.54 0.68 0.13 0.48 0.74 0.23 0.38 0.84 0.27 0.34 0.38 0.07 16 05-17-05 0.50 0.60 Q05 3 031 0.70 0.07 033 0.68 0.13 0.48 0.73 0.23 0.37 0.83 0.27 0.33 0.87 0.08 17 06-07-05 0.62 0.60 0.05 Om 0.70 0.07 0.53 0.6S 0.13 0.48 0.73 0.23 0.37 0.83 0.27 033 0.88 0.08 18 06-22-05 0.61 0.60 0.05 051 0.70 0.07 0.53 0.68 0.13 0.48 0.73 0.23 037 0.83 0.27 033 0.88 0.08 19 07-12-05 033 0.60 0.05 031 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.23 037 0.83 0.27 033 0.37 0.08 20 07-19-05 0.62 0.60 0.05 031 0.69 0.07 053 0.67 0.13 0.4S 0.73 0.23 0.37 0.83 0.27 033 0.87 0.08 Note: 7id IC,, day25% Mi'hJ emnaenrtim An eastini ofthc coteenftitOn ofpotaim Chloride that would cane a 25% reductin in Pimephals growth for the test popUitbLn CT - Central tenadency (mean IC,,). S - Standard devio of the MiC.vkeas. Laberatery Central and Wanint Limts LAbonteacostrolnd wa2ring lnwere tablinhedusigthe rblldard devi ofthe IC svaes conapondngto the 1th and 25th percnt CVs. Thes rangs ar more stribnt thta the contol and waninglinks recommended by USEPA for the teat method and endpoint SA - Standard detion caneApodinc to the 10' peretile CV. (SA - 0.12) Sus - Standard devitiba correspondkkg to the 25' oPercentie CV. (SA1 - 0.21) USEPA Centrol and Wazidn Lhntba SA.n5- Standard deviatim codrespoading to the 75' percentile CV. ( S,, - 0.38) SA"- Sbndard delobn comiponding to tbe 90' perecntile CV. (St -0.45) CV = Coefficient of aiation ofthe XC2,values. USIIPA. 200QW4 nd thSriMtt o bi iy tStlbenticlypptutb dstatath arobhtatkP im. Ets-. llU-xxninmtaAenchCc.tL Orlt.M .he@tafroZw at.:rl. I 07 1w4
Environmental Testing Solutions, Inc. PimephalespromelasControl Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25 , , , I , , , , , , , , - I , , I , , , _ 1.00 _ C5 0.75
.6 .E 0.50 f - - - -
v i0.25 2.... per s n a ..... USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)
- I I I T l B I r B r rI I I B I I I -
~~30 Kentucky Acceptance Limit (< 30.0%) o _~~
~~- v 20 ........ ....... .~~
' O~ co (10 o 0 .......... ........... .... ... .................. ................................................ ......................................... 40 USEPA Upper PMSD Bound (90e percentile < 35.0%) 30 1 20 1 10 ................. ..................... ..................................................... I.I... .. I . I.I..I.I.I..I.I.I..I.I.I..I.I.I..I.I.. aI a 0k 3v- t1.- 'VOJb , , pA5 , ' 1 , ' , Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
~~- -- Central Tendency (mean Control Growth, CV, or PMSD) . . Control Limits (mean Control Growth, CV, or PMSD k 2 Standard Deviations)~~
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean CTv CT MSD PMSD CT Survival Growth for Control Growth (%/) GrowthrCV (%)( ) forPMSD () (gare) (mgllarvae) GorCowth o (.V) o(PSD(% 1 11-30-04 100 0.689 15.7 0.10 15.1 2 12-07-04 97.5 . 0.687 0.688 7.6 11.7 0.09 12.7 13.9 3 01-11-05 100 0.797 0.724 8.7 10.7 0.10 13.0 13.6 4 02-08-05 97.5 0.687 0.715 3.9 9.0 0.11 15.5 14.1 5 02-12-05 100 0.712 0.714 5.8 8.3 0.11 16.0 14.4 6 03-01-05 100 0.749 0.720 11.8 8.9 0.17 22.7 15.8 7 03-01-05 100 0.889 0.744 4.5 8.3 0.12 13.7 15.5 8 03-08-05 100 0.735 0.743 8.3 8.3 0.08 11.0 15.0 9 03-15-05 100 0.533 0.720 3.1 7.7 0.06 10.5 14.5 10 03-29-05 100 0.676 0.715 12.5 8.2 0.12 17.4 14.8 11 04-12-05 100 0.702 0.714 9.8 8.3 0.10 13.9 14.7 12 04-26-05 100 0.742 0.716 3.9 8.0 0.09 12.3 14.5 13 . 04-26-05 97.5 0.702 0.715 7.2 7.9 0.10 13.5 14.4 14 05-03-05 100 0.760 0.718 2.5 7.5 0.10 12.9 14.3 15 05-10-05 100 0.772 0.722 10.7 7.7 0.10 13.5 14.3 16 05-17-05 100 0.858 0.731 6.0 7.6 0.06 7.4 13.8 17 *06-07-05 100 0.848 0.737 3.3 7.4 0.09 10.1 13.6 18 06-21-05 97.5 0.622 0.731 7.2 7.4 0.09 14.5 13.7 19 07-12-05 100 0.765 0.733 5.6 7.3 0.10 13.4 13.6 20 07-19-05 100 0.596 0.726 2.9 7.0 0.06 9.7 13.4 Note: CV- Coefficient of variation for control growth. On average, the CV for control growth is 7.0% in Environmental Testing Solutions, Inc. Pimeph.es chronic toxicity tests. Lower CV bound determined by USEPA (10e percentile) - 3.5%. Upper CV bound deternined by USEPA (90' percentile) - 20ff MSD - Minimum Significant Difference PMSD - Percent Minimum Signifcant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Envirommental Testing Solutions, Inc. cronic toxicity tests when a toxicant reduces Pinephales growthby 13A% from the controL Lower PMSD bound determined by USEPA (10' percentile) - 9.4%. Upper PMSD bound determined by USEPA (90' percentile) - 35%. CT - Central Tendancy (mean Control Growth, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 labontories for Pimephales growth in ehronic reference toxicant tests. USEPA 2000. Undertanding and Accounting for Method VriabiLty inWhole Effluent Toxicity Applications Under the National Pollutant Dischrge EEmimtion Programn EPA-833-R-00-03. US Envirmnental Protection Agency, Cincizmati, OR Organisms obtainedfrom Aquatc BfoSystems. In0 07 19-05
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~~Potassium Chloride Chronic Referen 'ce Toxicant Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespronmelas 1 .,.F ..~~
. :. .PKCICR Test Number. 10 I.. I Dilutionpreparationinfo mation: Comments:
KCl CHM number WM te _ Stock preparation: 50 g KC1/-L Dissolve 50 g KCI inl-L Deionized water Dilution prep (mg/L) 300 450 600 750 900 Stock volunw(fl) .6 9 12 is IS Diluent volunw(mL) 994 .991 . 98S 9S5 982. Total volune (niL)0 1000 1.00 1000 Test organtsminformation: Test information: T Organism age: zz.S -rM wt~ogS t. Randomizing template: Date and times organisms i*Os 0 11; :T tSOC Incubator number and were born between: shelf location: 3 Organism source: PTBS XE (SFIFOS Arsemia lot number - 7.. Transfer bowl information: pH = SU. Temperature 'C Total drying time: _* 2 .3 Date /Time in: ava oS 1310. Average transfer volume: Date / Time out: tn-115-6 3
.___ ___ _ .__ Oventemperature: _____ .V.I. . : .
~~Dailyfeedingand renewalinformation: Day Date Morning fceding Afternoon feedingtUme Test Initdation, renewal, or Ms batch used Analyst'.~~
~~. time . termination time 0 0 11°-CS __ _ 1530~~
~~_____ l 3Z. _______ lei_~~
~~- G-A O13 2~~
oizic 2leSC~s3 I~~l l32-.. 61-i3-o5R : 3-27-0S ffROS %Soo %15 O >3.. 4 Cl 23-as CRtOO sq131 c 3o S C_-24_ CS WA1ZI I IZS5 oi3.oSA . L. 6 m*2s-aS 08 . 12440 A 7 al *L*OS '; g:21301 \ Control information: Acceptance criteria Summary oftest endpoints: Morali: s 20% s_ 7-day LC50 1L1. 6e Average weight per initial larvae: _ p. 0q ( NOEC 3OC Average weight per surviving larvae: _ 2 0.25 mg/larvae LOEC 300 IChv _h_ . 1 -
~~-14ev) b62 .5~~
~~Environmental Testing Solutions, Inc.~~
~~Page:aS* ..~~
X..;Z.. Species: Pimephalespromelas PpKCICR Test Numlb.r . : . .: Survival and Growth Data Day _ Control 00 m KCIL - m KCIIL _____A B IC D 1E IF G H I J K L 0/ 1o 10 /0 _ _10 10 I0 to10 I_ lD _ ID I 10 1 I- - 1
~~-0 10 .2 3 1 O {~~
1/0t10o10 0 1 C IttoO /1 100 O 1 IIC) to C) l 3.to 4l J to /0 (010 Io0 (0o 10 10 1/ 4._ _ 10 iO ' 10 t O0 10 t 10 10 6t_ o 1 iC _ lC) it) to 1to I 10 6 ID tO 110 to el I00 10 ~ 0 tO IL) A - /_ . 10 _0 to 0 /0o to A Pan weight (mg) RAM
~~'Trax color eqd: 152. K1s.7 I 16Z 3 I. ° q iq.sqt 15.-tS K'l82- 15e19 ISJ Z 1(..! yji.q2. .1..q Analyst:i_~~
B - Pan + Larvae weight (Analyst: L.. al.'S aa. 02 ,i.ao 1141 a1J-. [i7 a,*7 0f l ao ao D Larvae weight (mg) - A - B 5tq S. tb 1q. 535lZ;.os S~os qjsn !slt le.A :5.it Weight per Initial number of larvae (mg)
-C I Initial number of larvae C2 0@ "Oil, I0* - - S - I - *- a - - -
~~Average Percent I weight per~~
~~.i .4 .. '~~
reduction Initial from control 0 5 1 6 0 *SZ1 number of (%) I rvac (mg) J. a- I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:
~~... '.,of Page 3 of'5.^ - <~~
.:.: Environmental Testing Solutions, Inc. Species: Pimephalespromelas PpKCICR Test Number: - O Survival and Growth Data Day 600 mg KCI/L 750 mg KCIIL 00 KCVL _M N O P .2 R S T U V WIX O. ID IO /10 6 1o1 001 I0 1 J I0 1 0 i_ 10 .i Fi H 41-A sl 3 3 2 _ ' 10 I_ 1 o 9 5 3 3 3 4- -o - ` - - - - - 53 _-- so1 1 5 g 94 6. 4"4 20 z'4 6 & ? L, 7 Ir LAW~ _O & _ 6 Ia &f '2_ A - Pan weight (mg) _ Lb Tray color code:: P . Iq.13 16.1 .24 tL2i. 15f t01 15.O s5.1i j i.K qA l1 4. 3 tq.L A:alst1:~z B - Pan +Larvae welght Analyst: _ _, ilk o ID . S I5Yf i4J _ Larvae weight (mg) - A - B qA4 A4. 4.17. S*ot s.01 3.3>1 14.I'i '3.5% 0.qq 1.10 o.-s _ Weight per Initial number arlarve (mng) h % \O dy
-C J Initial number of larvae O O O O O O ' v Average Percent weight per redaction Initial roenctontr o.4lb3Z65 as' 43
~~o. 32. o. 06 c 37.~~
number of (%) larvae (me) . Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r - decanted and returned, w = wounded. Calculations and data reviewed:4 Comments:
Environmental Testing Solutions, Inc. Chronic Whole Emuent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Plmephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test maban PpKCICR I I st(a79 at 351 DepotSt.) Teat dateel Juy mid, 200 Reveiwod %Y/ '~ C-as 3.4. aq.V* Ialedalaoo l 3r r".b A
6-NdM J - w.L L-.sw.60 wo W*U113w.29 atem ws.*d Cwf0~. dn wopas 3oo a. 9t0 H-- N H .ughU dbhC~dwvi-a twd.,w Km - 4 1*O( d -A. 3 6dwL boborhm. h11 to1n 1_%) CA4) 4M1 A 10 10 15.20 2104 54.4 0 34 0.34 cota a t0 t0 13.32 23)15 6.03 0 60) 0.596 190.603 200.0 *34% 23 No.t ppllubk C 30 10 16.23 22.02 3.79 0.379 03 79 D 30 30 15.04 2120 6.16 0616 0616
£ to 9 14.54 39.47 4.93 0.54 _ 0.493 30 F 10 30 33.33 2027 5.12 0.512 0.51? 4.0 0.512 9ts 0e54 IA I5.4 C 10 30 14.82 19*7 S.0O 0.S0O 0.0SO U 10 10 IS.I9 2024 5.03 0505 0.S05 T o0 9 15.12 1990 4.78 0.33 0.478 40 J 10 10 1614 2130 3.36 0.516 0.541 t.0 0.606 n s JS7 to.2 1 ts I 30a 3I0 34.92 2096 6.04 0 604 0.604 L 30 30 14.96 2007 3.33 0..SII 0.3s
_ 4M 30 30 14.73 1967 4.94 0494 0494 N I30 7 36.39 -2063 4.44 0.634 osso 1. 0.444 1330 0A3 931 22.3 e 30 e 35.24 19.36 4.32 . 0.3n 01432 _. 30 9 16.17 23.I 5301 0355 .O.S 0.50 Q 10 6 IS 95 1902 3.07 0.532 0.301 75t It 10 16 01 19.33 3.37 0.421 0.492 9.7 0.337 7n.s 6J5SS 1a3 40oa
~~- 10 a 15.23 13947 4.19 0.524 04139~~
_ __ _ _ _ T 30 I IS.M 1169 3.38 0333 _ _ _ __ _ _ _ __ _ _ _ _ 0.3335_ __ _ _ U t 4.46 149 049 0.490 0049 V t0 2 3 .9 030 O. 0 430 36.9 0.110 1,. 12 MI 0.U41 W 30 I 1453 14.7 0.25 O 0025 X 30o 0 000 000_ 000 0000 _ _ _ _ _ 0000 I _ _ _ _ _ _ __ _ _ _ _ _ _ Dtt'1 MSD VAct 0.037S MSD - Mfbib Stpignuct Difiome-e I'MSDI 9.7 33.SD- Faver4 Minimro SipU~kont Difference 9PeS.4Dsawe san of tti p ciion The tMSDtheminihmm p rentdiffenC etweewthe trl d thgt esabedecleds wsticfyll rticusg h osol Junttoxcitt . Ot aSr; dm slrerns a TroandSonerotosb dhonic toticxotyt at r es im l g byy1 .4% m thecontrot (detmubed ftho h ftutu touiant tentl6g} LowtPMSD bound etmined byUSEPA (ItO pnule) - 9.4% Upper MSD bosad detrmned byUSEPA (90th pecntle) .-33% Thelowerandu bpperbunwe eicultebytheUSEPA uings20tests D od educoot 19oaeo rodsfor imephale gowtin rna re nceKtoxkmte . USESA. 2000. Udrmnding wi Atootetio for MethodVarfsIai nWhote ny eentTouccy A~pflcdn nderthe National Polutol DVcltrhare Elimination Propun. EPA-833-lt0 US EnvimentA P3otecon Agency.CnsdscmIl. Oxezarwt obeelanedfrowAvetk SoSyoInt. Inc. 07.19-05
Environmental Testing Solutions, Inc. Statistical Analyses
,, _LMFish GrCb sad SorvTesit.7 DaySarival SaxtDat: 7WM=0 Test ID. PpKCR Sample 1DP REF.RxfTdxkanl End Dtet 7/2n62co Lab ID ES.m k Tatt SO1 Sa-ple pc KCLPoubmj el Sample Data: rotocol IWCHRI.PAW 2. 2.R2013 Test Spect : PP.?imephilm Pecehs Comments:
Caea-mflL I 1 5 4 D-Cool 1.0000 IJO lOXDO 1.0000 300 Oxw~ 1.0000 L0000 LWOOD 450 O.0 L.o l 1.OX l.00X 600 1.000 0.7000 0o10 0.9000 750 .600 QIC 0.1000 O 0.7000 900 0.2000 000 0.2000 0.00o Trufar= Aruh Square R.st Rank I-T" Number ToW CaeumzlL Mesa N.Mesa mIes MLb Mle CV% N Sam Cided Rasp Nomber DOCoolr 1.0000 1.OXO 2.4120 2.4120 1.4120 0.00O 4 0 40 300 0.9750 0.9750 13713 1.2490 1.4120 3.42 4 16.00 20.00 1 40 450 o9750 o.97 1713723 2490 1.1220 1942 4 2600 20.00 I 40 600 01500 0a1500 1.229 099l2 2.4120 11.51t 4 22.00 20.0o 6 40
-70 0.7250 0.7250 1.0229 0.1161 11071 10W36 4 10.00 2oD0 12 40 *9t IO.CO 0.'00X 03265 0.1521 0.4636 39374 4 20.00 10.00 36 40 AuelliuTlats Stadafe Oleical Skew Kart ShapomW&s Test ndicatenmldotutiso (p> 0.0) 0.94493651 0.04 4132957 0311192149 Etuabry of vne cuonnt be ecwfimed Ityedeals Tat (1.tll. 6.99) INOWC LOilC OV TU Steals lis4ny 6 RankTest 600 750 670.8203932 Trnenta u D Coatlol Maximem Lk&Dlba4r.bleb Pramter Vale* St ffFUYldseWLbu COMl 012.Sq Citile rPNaub. Me SiRs liar Slop. I.103164432 5.754692465 *10.21013526.41716324 0 63.8623447 7.11472713 1.2-14 281479048 0.12340578 5 ltercept .11.3759316 16.a30L09 .70.663551333S169411 SCR 1.0 Palx: Prultbi c gL 95% FPaelt imt /e Ea=t 2674 396.0016561 EC05 3355 410.6195412 0.1 ECIO 3.712 532.3211041 0.7 EC15 3.964 S71327273.
2020 4.152 603.1474035 0.6 EC25 4326 633D71361 01 E0WM 4.747 713.7157421 I EC50 .000 766.991371 0.4 EC60 5.253 224.2A43091 03 Ec75 5.674929.069252 0 EC3O.142 97421264710 E15- 6.036 I20.663367 0.1 EC90 6.212 IIC3391t6 0.0 * - EM95 6.645 1225394636 I . . 100 1000 10000 EC99 7326 1415531305 t ntgtO SgaUSOM hUlsgeele y deWCstd (p - t7ne.24) Dae-Respea nda Orgnodou vbkaedfivm ApaewttBioSystatme lao. 71.O 07-I94
Environmental Testing Solutions, Inc. Statistical Analyses larva Fil Crrh ad Sertival 7 t.7 DsYCr"wU Sent Date: 719/2005 st ID. pPK= 3amplI R1EP.RdTaxiut 2ndData VW22005 Lab M bik.rstigSoI Sampk )pc KCCLPokttica Sa. ple Datec Protowt rWC4R.UEPA t21-R42413 Test Specis: PP.Pimphaks ptwuiali Cs r-.L 1 2 3 4 D.CockoI 0.50140 6030 0.5790 06160 300 0.4930 0.5120 0.5Q50 05050 450 0.4720 0.5160 0.6040 W5110 600 0.4940 0.4440 0.4120 0.5010 750 3070 0.3370 0.4190 0)510 900 0.0490 0.110Q 0.0250 OO Trhjfam: Vatbsmismad 0-Taiblld 149.sKc C"te-/L Mess ?4Me"s Meas MIX Mal CV% l t-Stat Crticl MSD Mea N-Mes D-Conl 0.5955 I CWO 0.5955 0.5790 0.6160 2U70 4 0.5955 1.09
*30C O5030 0.459 'O.5031 0.4930 05120 1.566 4 3653 2.290 Q.0575 0.5155 0.1657 *450 0.5273 .54 Q52m 0.4710 0.600 10.211 4 2717 2290 0.575 0.5155 0.1657 *6C0 0.4622 07771 04621 0.4120 0.5010 9.140 4 5.215 2290 O.575 0.4628 0.7771 750 0.3553 0596 0.3553 03070 0.4190 13335 4 0.3553 0.5964 900 0.0460 Q.0772 0.0460 0.MW 0.1100 11 3 4 0.0460 Q00772 AsxilsryTests Stsdsrc Crtical Skav, Klrt SbapksoWtls Tet ktdmutanam dt datribum (p > 0.01) 0.92534149 0.144 0.66152367 1.2594l343 Butlers Test kicates eqalvarnces (p 00.03) 1.652140614 U134496675 Rlysthesis Test (1.099, .OS) NOEC LOWC CV TU MSDv MSDp MSS MSE F-Preb dO D nattrsTest <3C0 30D 005751s92 0.09653*926 0.012364065 0.001261771 0.001507935 3.12 SnetacftVs h D.4miftl Llssarlstbtepelsda 200 Reswnples)
Walit NW/L SD 95%C.Krp) Skew JC05- 111.66 32.97 71.74 219.20 5.1034 lC10' 22331 71.41 143.47 61537 23752 IC15 476.52 99.14 130.21 610.63 44975 1.0 IC20 561.10 4225 436.11 667.74 44525 IC=5 62250 26.5 520.97 619.44 Q4526 IC40 747.14 16.42 61731 7U.24 4Q4213 0.D8 IC50 777.9 7.5 752.91 10331 0.021 7/
~~hkdatu IC a ta less1km Ut, 0,wCst wns ttn 0.5 0.4 0.3 0.2 0.1'.....~~
0.0 0 200 400 e00 Soo 1000 DoDRnmt Dss.-aspsas Fi_ Otaafrig obedned from AquaicBaSysfcmr. Inc. 07-19t05 719O
EnNironmental Page 4 of 5 Envi4-~~~ ~ ~TestingTetn
~~-~ronenaSolutions.~~
Soui-s Inc. _nc _ __ ___ o __ __, Species: Pimephalespromelas PpKCICR Test Number: _2f IDaih, Chemiktrv: Day
.Cr 0 1 2 Analyst IF xel A57 Conctntratlon IParameter i lg CONTROL pH (S.U.) +.0-Y R I -f- --t-* I t.-b ( I/..6,5S I Dfl)(mzUL T7-.q--1 1 7/ 1 2- Te I-4 A,44' Con luctivity (Prnhos/cm) 2 0 /1 Alkalinity 0 (mg CaCQOL)
Hardness (mg CaCO3/L) ____ Temperature - EC) 2COO1A I4z-S' 124.n I 0 (S.U.) pH_ -C _ 24. - ?.. Z 24 C DO (mg/L) .4r2 0 .<S;L]99Z7,
~~,,-f 300 mg KCI/L Conductivity j?~~
~~Temperature~~
Z4A 8 2b1. zi z{ 271 4 Zz pH (S.U.) ?-7fV .wBX2TE s 1-7S DO6(mgl Co.duct L)A:L 450 mg KCI/L Conductivity g ______
Temperature 22A* 27.3 z'I.°i 2{1 Zq 3 pH (S.U.) 775£ s'789^.2-i, -7, DO (mg/) __A 6 XZ?.gO ~g 600 mg }CiL Conductivity Temperature 21- O~ S. () JC5Zl N.( pH.SU) ?,3 Ff ?w9 /:; 73 -8.
~~.DO (ma/) t9' 9S% -1 75 7@ ;~~
_______(OC) 750 mg KCIL Conumhsct) /ty/ A5V x 4P) Temperature '2l.& Zq3 'ZS. ° A22d 24-1 -2wi _______ (Vmhos/cm) Z 6 pH (S.U.) 95- ;>. I. . ~~DO (mg/L) A OI 5 .27 7...94 -9 i,15:,:t 900 mg KCVL Conumhsctivityt~ R ns I Temperature 24.1 2I.s 7 3 l 2q .-1 Z e I STOCK Conductlvlty 632-00 Initial _ Final
~~;7° I Initial I Final I Initial I Final I I~~
E~nvironmental Testinp, Solutions. Inc. Page 5 of S Page 5 of 5 Environmental Testing Solutions. Inc. Species: Pimephalespromelas PpKCICR Test Number. 10 _ 4 ________Analyst N I Concentration Parameter - V - 4 CONTROL pH(SU) DO (mglL) Conductivity X-i ____ _1.69_7.C5 _7i77 O7 -I X1, 1 2 a (gmhios/cm) l Alkalianity,)wMW1ss (mg CaCOAI) Hardness (HmagrCnaeCO L) 3 - mm g2 Temperature 24.5 24W'i 25b 1 sA 4.3 pH (S.U.) ^6 1 .S .9 4 7498 7 v.S ;,f DO (mg/L) ).7____ ? 300 mg KCVilL Conductivity Temperature q 24A 4 25,\ Z . _ __ _ _ IQ _ _ _
. pH (S.U.) ~9 C, 77 (L -( 7 . 7'. S9 T o7 O..?r ? .s .DO (mg/L) q K >3!n.C4 . 7 5 :jf i 1 450 mg KCIIL Conductivity (gimho /m) 0l 0 ll
_____0 film___ III0 ___ _____ Temperature 2!-j2 z4*2 Zq.1 Zl.q .s -zQ.5 2S.2 211. pH (S.U.) .SS .7a .l zg 600 mg KCiL DO(mg/L) . .0 1. 1 . Ls +. 2 f(^ C_ Conductivity a o/300 (uimhos/cm)- _ _ _ _ _ Temperature 2Jq.2 Zq. l1 pH (S.U.) ; 2 97 n 1 1 T1..511 - .j9 ;Z, 9 S b. G DO (mgtL) ,g w 4rt4P I - f Z :7a i 750 mg KCL'L Conductivity (~Mos m) __ _ _ __ _ _ _ _ __ _ _ _ _ _ _ _ _ _ Temperature 0
~~?Z_ 24t3 Z 2'. 2S*O Z7.2.~~
( c) 3 zq k 24 Z - ______R'4 ______ pH (S.U.) X-6 7.73 7. i 7 .7 .Ai9?-X :ZTEoL _15 DO (mg/L) Li{ @7-2-7.a.l b ?, 900 mg KCi/L Conductivity (jimho /m) _____ _____ ____ M S_____ _____ Temperature i '24.q s4 2.o Va 2S.( zq.Z STOCK (Conductivity ____(pmhos/cm) I lOO ___ Initial I Finali InitialI FinalI Initial 11 Final . Initial _ -Fina I .1I Initial I Final Initial Initial 1 Final
Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.12 I I I I I I I I I I TI T_ I I i I USEPA ControlLimits (I 2 StandardDeviations) 1.10 1.08 1.06 F
~~.......... I..... . . ....................................~~
V 1.04 I-I I I I I I I I . I I .I I I I I I . 2.5 I USEPA Warning and ControlLimits (75th and 9 0 h PercentileCVs) V 2.0 1
~~4 ...................................................................................................... ..............................................~~
1.5 I 0-U 1.0 I C: 0.5 I ..................................................................................................................................................... 1.4 Laboratory Warning and ControlLimits ( 10 2h and 2 5 'hPercentileCVs) 1.3 1.2 1 ... 1.1 1.01 0.9 1 0.8 o' ok O .V' OS Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
~~- -- Central Tendency (mean IC25)~~
~~Warning Limits (mean IC25 +/- SA10 or SA.75)~~
~~. Control Limits (mean IC25 S S or 2 Standard Deviations) 25 A1_A.~~
Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toilcant Control Chart for Cerfodaphnia dubia using Moderately Hard Synthetic Water State and USEPA Laboratory Laboratory USEPA USEPA Test nunber Test date 7-day IC CT S Central lnit, SA. WaNiln Llits SMS Control Units SAj Waning Liits SA. Control Units CV (g/LNaCI) (C/LNaCI) CT-2S CT+2S CT* s ,, CT +SA CTr SAs CT + SAM CTr-SA.5 Cr+SA.S7 CT - SAM CT + SAM 02-03-04 1.06 2 03403-04 1.07 1.07 0.01 1.05 I.OS 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 3 046-04 1.07 1.07 0.01 1.06 1.08 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.00 4 05.04-04 1.07 1.07 0.00 1.06 1.08 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.00 5 0608-4 1.06 1.07 0.01 1.06 I.OS 0.09 0.98 1.15 O.I 0.89 1.25 0.4O 0.59 1.55 0.66 0.41 1.73 0.01 6 07-07-04 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 7 08-10-04 1.06 1.06 0.01 1.05 L.OS 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 S 09-14-04 1.05 1.06 0.01 1.05 1.08 0.0O 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 9 09.14-04 1.07 1.06 0.01 1.05 I.OS 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 10 10-05-04 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.4S 0.59 1.54 0.66 0.40 1.72 0.01 11 11-02-04 1.06 1.06 0.01 1.05 1.08 0.09 0.9S 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 12 12-07-04 1.0O 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 13 01-11-05 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 14 02-08-05 1.05 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 15 03-01-05 1.08 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 16 04-055 1.09 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 17 05-03-05 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01 IS 06-07-05 1.05 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 19 07-12-05 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 20 07-12-05 1.06 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 Note: 7dICis- 7-day25 inUbitionconcentration. An estimsaon of lte concentration of sodiumechorlde ohat would cause a25%reducton in Certodaphnta reproductionforthe test population. CT - Central tendency (tcan Ci,). S - Standard deviation ofthoICs values. Laboratory Contyul and Wani LlUnits Labortoycontrol and waning limitswer esblishod u-ing theodandrd devation ofthe ICI n1uesaconuxponding to th 1Oth and 25thpementile CVs. Thse ruges ar nn stringent than thoecontrol and arning limita reconmended by USEPA for the test netled and endpoint SAss - Standarddeviationeorespodingto the l0 0pcreentile CV. (SA,- 0.08) S^., - Standard deviation correynnding to the 25&percentilo CV. (SA21 - 0.17) USEPA Cortrol and Wanint Lnits SAYs - Standard deviation corresonding to the 75* percentile CV. (SA.,s - OA5) SA - Standrd deviation corresponding to thG90o percentilo CV. (SAr - 0.62) CV - Coefficient ofvariation of(lie IC, 1 values. USEPA 2000. Undentanding and Aecoiming forMtMthd Vriability in Whol EffluentToxidcity Applications Under thoNational Pollutant Discharge ElitnntionPogram. IEPA-833-R400-003. US rnvitonmental rmtecton Agency, Cincinnti, 011. 07.1e.03
Environmental Testing Solutions, Inc. Ceriodaphniadubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40
~~. 35 . ............................................................. ..........~~
~~r 30~~
~~.....2s. ............................. ....... .................................................... . . . . . . . . . . . . . . . . .. . . . .~~
20 15 -- Minimum Acceptance Criteria (> 15.0 offspring per surviving female) I I I I I I I I I I I I I I I I I I I I _I I I I I I I I I I I I I I I I I I I I s:4 _.._.._._.._.._.._.._.._....._...._
~~= 4 ~North Carolina Acceptance Limit (< 40.0%)~~
c! 30 ...... S. P: Kentucky Acceptance Limit (< 30.0%) o . 20:: 10 ...................... I.- U I I I I I I I I I I . 4e0 I I I I I I I I I I 40 I I.. L8 I I I lI I I 1 1 1 1 1 1 1 USEPA Upper PMSD Bound (90* percentile < 37.0%) 30 20 10 ........................................................... .............................................. 0*Ito I rpI Tes I daeI I Test date
-.--@Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
~~- -* Central Tendency (mean Control Reproduction, CV, or PMSD)~~
~~.. Control Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)~~
Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Tes Test ate Control Control Mean cr CV CT MSD PMSD cr number Survival Reproduction for Control Mean for Control (N) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%/) (offspring/female) CV (0/.) 1 02-03-04 100 30.7 7.2 2.7 8.8 2 03-03-04 100 29.0 29.9 7.8 7.5 3.0 10.3 9.6 3 04-06-04 100 28.1 29.3 6.2 7.1 2.8 10.0 9.7 4 05.04-04 100 32.5 30.1 6.4 6.9 3.3 10.1 9.8 5 06-O-04 100 32.9 30.6 6.8 6.9 3.0 9.0 9.7 6 07-07-04 100 33.3 31.1 6.3 6.8 2.6 7.8 9.3 7 . 08-10-04 100 27.4 30.6 5.8 6.6 2.3 8.6 9.2 8 09-14-04 100 28.7 30.3 4.9 6A 2.4 8.2 9.1 9 09-14-04 100 28.8 30.2 7.5 6.5 3.0 10.5 9.3 10 10-05-04 100 29.6 30.1 8.2 6.7 2.8 9.6 9.3 11 11-02-04 100 30.5 30.1 8.5 6.9 2.7 8.8 9.3 12 12-07-04 100 31.8 30.3 4.9 6.7 2.5 7.9 9.1 13 01-11-05 100 31.0 30.3 9.4 6.9 3.1 10.0 9.2 14 02-08-05 100 30.0 30.3 6.8 6.9 2.4 8.0 9.1 1S 03-01-OS 100 29.9 30.3 6.6 6.9 2.9 9.9 9.2 16 04-05-05 100 32.4 30.4 6.9 6.9 3.1 9.6 9.2 17 05-03-OS 100 29.9 30.4 7.0 6.9 2.9 9.8 9.2 18 06-07-05 100 30.4 30.4 5.0 6.8 2.6 8.5 9.2 19 07-12-05 100 30.4 30.4 7.5 6.8 2.7 8.8 9.2 20 07-12-05 100 31.1 30.4 7.2 6.8 3.2 10.2 9.2 Note: CV - Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.8% in Environmental Testing Solutions, Inc. Ceriodaphnfa chronic toxicity tests. Lower CV bound determined by USEPA (10' percentile) - 8.9%. Upper CV bound determined by USEPA (90' percentile) - 42% MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mnnimum percent difference between the control and treatrnent that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Sohrtions, Inc. chronic toxicity tests when a toxicant reduces Cerodaphnlareproduction by 9.2% from the control LowerPMSD bound determined byUSEPA(10' percentile) - 11I%. Upper PMSD bound determined by USEPA (90' percentile) - 37%. CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD) Tle lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Criodaphniareproduction in chronic reference toxicant tests. 07-12-05new
Enironmental Testing Solutions, Inc. Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnladubia NePIJ AL6Ae CdIXaCLCR #: 38 Dilution preparationinformation: Comments: NaCI CHM numbers . H 1Cl _ Stock prparatiort 100 g NaCIl (dissolve SO g NaCI En 500 ml deionized water) Dilution prep (mgfl.) 600 800 1000 1200 1400 Stock volume (mL) 9 12 I 15 I8 21 1 Diluent vohame (tL) 1491 1488 1485 1482 1479 1 Totl volume1(5L) , IS00 IS00 1500 1500 1500 1 _ 1 Test organism information: Test information: . Organism age: < 24-hours old Randomizing template: JKAQ Date and times organisms CA-i- l- l J5 -t3 ZeCOG Incubator number and I 7 were born between: . shelf location: ___ Organism source: ' a-oS-0oS A-F YCT batch: 0I _2_-aS Transfer bowl information: pH = 1,.1 SU Temperature = . CC Selenastrum batch: ______ Daily renewalinformation: Day Date Test Initiation, MHS water Analyst renewal, or batch used
. _ termination time 0 a -_ _ _ _ _ _ ai6o s-c A t el: n os MR on-o8-. s 6 2 _-__-OS__ AT l o-o -oS C.C 01-17-6 Oa t)&o 01 - G&-ci' C_ .6 .- 1903-05 oS 7 iro __ Olt maim.
~~Controlinformation: Acceptance criteria Summary of test endpoints:~~
% of Male Adults: Z ) i S20% 7-day LC50 >',IOo) % Adults having 3 ' Broods: 1007i a 80% NOEC EOO % Mortality: aS, s 20% LOEC 1000 1Mean OffspringFemale: , .l 15.0 offspring/female ChV .1 %CV: _____ <40.0% IC2S I257.9
Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphniadubia IWJeSJ AL6A& CdNaCLCR #: a CONTROL Survival and Reproduction Data RepUcate number Day 1 2 3 4 5 6 7 8 9 10 Adult mortality l. L l Adult mortality 3 Young produced - ) l_il_C S Adult mortality T T1 l1 4 Young produced 's ^S Li S 5 4l45l5E S Adult mortality ! C - \- :- L <1
~~\. \.-~~
~~S Youngproduced Ir, ,0 0 I ' IT z I~~
. Adult mortality l l1 6 Youngproduced l 1 3 C) l O Qi L iO I Adult mortality U C. "-- -- L 7 l Young produced Total young produced Final Adult Mortality* I -z C) 0 29 L
~~l1 35 lZ. 1 L- l 30~~
~~-I %.5= l 3o Il l 3 II 32.~~
L-l3 C) ll T D Xfor3' Broods l ll1^ >'X, CI I Note: Adult mortality (L - live, D - dead) *t CAf lk olete Concentration: l %Mortality: I OMo l Mean Offspring/Female: l 3,1., 600 mp-NaCL/L Survival and Reproduction Data Replieate number Day l I l- 2 3 4 5 6 7 l 9 10 1 Young produced 00 0 D 0 Adult mortality . l L L. I U. -l
2. Young produced j ) 0 0 3 Adult mortality lYoung produced U . UC a U UIL . 09t 4 Young produced 01 7 Lk Adult mortality U* l . L - U -
s Young produced l - 1.- ( l l Adult mortality l L l ll X C 6 Young produced l f 0O 10 1 lj 0l I S l...LO, 67 Young produced Adult mortality L- L5 *~ . (0 Ct) - DM r -0.L.. S I Totlyoungproduced l f3 j 31 j 0 0 0
- 1 - - - - - - - 30 Final Adult Mortality U . ~ ~ W T Note: Adult mortality (L - live, D - dead)
~~I Concentration:~~
%Mortalit: o l Mean Offspring/Female: 33oA % Reduction from Control: 2 .(o3
Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphniadubia tJNe AL6.4 CdNaCLCR #: I38 800 mr NaCI/L Survival andReproduction Data
. = Replicate number Dar 1 2 3 4 1 6 7 a 9 10 I Yon prdue O 0 O 0 C]
Adult mortality L l L - U U t _U 2 Young produced _ ) C C) I O OJ _ Adult mortality ! _ - ! - L- U 3 Young produced 1. I o. o) CC).*z c. o o Adult mortality l t_ 'l ' ' i - IC1 3 I I I1vS tq 5 1 1111 4 1 Young produced Adult mortality I . L I I . ' - S Young produced IC) 11 IO I\ 9 I = Adult mortality J ' L- tC L-6 Young produced (D) l _ lO o l 1 IS _d Adult mortality j l Il Wi%--1Lt L- L-U o 7 Youngproduced Totalyoungproduced Final Adult Mortality l lrZ TL 5 lL 5 T lIi 1 31l lE L l lC C tl3 20
~~-2 Q~~
~~LE -l 1I(D l Z1 l l* Z I l j30 I~~
~~-Cl 3~~
~~Note: Adult mortality (L.- live, D - dead) l Concentration:~~
%Mortality: l T l MeanOffispring/Female: _ 2-I.L %Reduction from Control: LI .R 1a I 1000 mg NaCllL Survival andReproduction Data Replicate number lDy l I 2 3 4 5 6 7 8 9 10 I Young produced - - -
J Adult mortality _l l . l. LU L. fT? 2 Young produced l) C) l _ p OO p Adult mortality U!.. U. ' U j ] l-!. 3 Young produced lOl O - l - Adult mortality TC .j . L U U 'I. L I U Adult mortality L Ll l l1 . L T? l Young produced [ l l F l 5T Adult mortality [ g
~~l ULE l Ll .~~
~~l C Young prodsuced Adult mortality 7 Young produced l jd t__U nI 14 l~~
~~+ lI OI i5 L-I I (g) i__ 3__ i_~~
l 13i O l C 0 0 112-Total young produced 211 zl - 2A 1 l 21 2T 2z 2 1 2 2.1 Flinal Adult Mortallty l L l W -l ll W 7 C Note: Adult mortality (L - live, D - dead) I Concentration:
%Mortality: l Cm I Mean Offspring/Female: I Z(- % Reduction from Control: I .870
Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphniadubia NJWJ AL6-Ae CdNaCLCR #: 3& 1200 mng NaCIIL Survival and ReproductionData [ . Replicate number Day l 2 l 3 1 4 5 6 1 7 8 9 10 y - -dult-mortalit L- L- m -L 2 Young produced l) a IQJ c. o 0 L-Adult mortality lL \ I - i.- L L L I
~~I I____ _ _ -Ie Cl _L C) 3 Youn prdced0 Adult mo y _~~
~~rtalit~~
=
0>o11 b° 4 Young produced l Il 1 taA I Adult mortality -- I \- Young produced ~ tA __ I'~ LA. Lk.... 6 76 Adult mortality Young produced Adult mortality Young produced I-l 6 U.. _I LlL 1 ( l
~~, ) iO l~~
_lLIL Il1 L-1-........... I L Total young produced IC)0 i 2 15 IL Z i Final Adult Mortality - I '-* l ' - - Note: Adult mortality (L - live, D - dead) l Concentratfon:
~~% Mortality:: 63%~~
i Mean Offspring/Female: I ltI~.2 I % Reduction from Control: 1si2 .'.4"7 1 1400 mg NaCl/L Survival andReproduction Data [ _ _ - - Replicate number Day 1 2 3 4 5 6 7 8 9 t1 I Young produced l C0 _ C) I --- ! Adult mortality ! .- - 2 Young produced 2 _ lOlOlC _n_ )lOlt )lUlOlC p p m Adult mortality _t - 3 Young produced l l 0 0 iG Q Adult mortality j T~i.. 4 Young produced L 3 I Li 12r Iz.. - - J Adultmortality L- 3 .- s Young produced l4 l L t 1IX 1 II 1 Adult mortality l U l l 1 L-l 1 6 Young produced 1l 0. 4 0. Adult mortality l l LLT I l L IL I 7 Young produced [lZ -. 0 5 I LAQ-I 4 -I I Total yIoung produced Final Adult Mortality i 1 U i L l Note: Adult mortality (L - live, D - dead) i Concentration:
% Mortality: 20l7. l l MeanOffspringFemale: l % Reduction from Control: 12.1-.
Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control 1000 mg NaCIIM Day ~Replicate numberToa DyRe pIlcate number - - Total Day j -- 3 4- 6 7 8 Total0 Da 1 2 13 4 S 8 9 10 I 0 0I 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 000 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 33 5 5 54 5 5 4 5 5 46 4 4 4- 5 3 S 6 4 4 4 4 43 5 12 10 13 10 9 10 9 11 12 10 106 5 9 9 10 10 10 8 10 8 11 I11 96 6 13 0 0 0 0 0 I 0 16 141 44 6 14 0 0 0 0 0 0 0 0 12 26 7 0 14 17 17 17 15 I18 17 0 01 115 7 0 121 513 131 13 9 14 0 103 Total 28 -279 35 32 0303 32 331 291 311 Total 27 22922872721 29 27 268 600 mg NaCl/L_____ - 1200 mg NaICI/L___ _ - D Day I 2 3
~Replcate 4 5 numberToa 6 7 9 oa Day Replicate number __ - Total 12314 5 6 7 18 9 10 I 0 0 0 0 0 0 0 0 0 0 0 I 0 0 0 0 0 0 0 0 0 0 -0 2 0 0 0 00 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 -0 0 0 0 *0 4 3 5 5 5 5 5 5 4 4 5 46 4 4 4 5 4 4 3 3 4 4 3 38 S 1I 10 10 10 12 10 9 10 I1I 10 103 5 8 6 6 4 10 8 7 7 4 4 64 6 0 0 0 0 16 1141 0 0 15 15 60 6 0 0 0 0 0 0 0 0 4 6 10 7 17 13 16 160 0 18 14 0 0 94 7 6 0 0 9 74 5 500 36 Total 31 28 31 311 33 J291 32 28 30 30 303 Total 181 10 11 171 21 i15 15 161 12 1 4 800 migNaCVL. 1400 mtg NaC-- --
Day ~~Re Uicate numberToa Day -R-p-j cate numberTotal 1 2 3 5 6 71 g- Tota I4 S 6 7 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 00O 0 0 0 4 3 6 4 5 4 4 5 4 4 4 43 4 4 3 2 3 4 3 0 2 02 23 5 10 11 10 11 9 9 9 12 11 10 102 5 7 -3 6 S 4 4 0 3 0 3 35 6 01 0 0 0 0 00 13 15 17 45. 6 0 0- 0 0 0 0 0 1 1 7 15 15 17 141 1 160 0 0 106 7 5 0 46340 4 0 0 26
~~'1otal 28 32 31 30 2 29 3 29 0 31 296 Total 16 6 1 14 11I 11 0 9 0 6 85~~
Environmental Tcsting Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphniadubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #I67(N3 at 351 Depot St.) UTEX Algae Test datets: July 12-19, 2005 Revelwed by: r Concentration _ _! Rept_ e number I Survival Average reproduction *akA
re dts.. (mm (mt/L NaCI) 1 2 3 4 5 6 7 T 9 10 (%) (offspringlremale) vsda (% Comml (%)
Control 28 29 35 32 30 30 33 32 33 29 100 31.1 7.2 Not applicable 600 31 2S 31 31 33 29 32 28 30 30 100 30.3 5.4 2.6 800 28 32 31 30 26 29 30 29 30 31 100 29.6 5.8 4.8 1000 27 25 29 28 28 27 27 21 29 27 100 26.8 as8 13.8 1200 18 10 I r 17 21 is Is 16 12 13 100 14.8 22.9 524 1400 16 6 12 14 0 0 65 643 727 Dunnett's MSD value: 3.165 MSD - Minimum Significant Difference PMSD: 10.2 PMSD - Percent Minimum Significant Difference PMSD Isa measure o'test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test. On avenage, i significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when atoxicant reduces CerfodapAnla reproduction by 9.2% from the control. LowerPMSDbounddetermined byUSEPA (10' percentile) - 11%. Upper PMSD bound determined by USEPA (90* percentile) - 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerfodaphnkareproduction in chronic reference toxicant tests. USEPA. 2000. UnderstandingandAccountingforMethod VariabilityinWholeEfuentToxicityApplicationsUndertheNationa PollutantDischargeElimin aonProgram. EPA-833-R-0-003. USEnvironmentalProteaon Agency, Cincinnati, Ofll
Environmental Testing Solutions, Inc. Statistical Analyses Cededspo*la S rvival and RepredvetUv Tel-jRepredvtles Stut Dte: 71122005 Tat HlY C4NaCIR D Sampl MI-RBF.RfToxzUl 3 End Dte: 7 51912005 lab ID: ETS-Eav. Teatig Sol UmpIDType: NACLSodiun Chtido Saple Dnte. Protocol FWCHIRB-PA-121.R402-013 Toot Specees: -Caerbdapbnhdubia Comment,: C.e-mig/L I 2 3 *7 9 10 D-Costmi 23.000 29.000 35.000 320ooo 30.000 30.000 33.000 32.000 33.000 29.000 600 31.000 21.000 31.000 31.000 33.000 29.000 32.000 23.000 30.000 30.000 000 21.000 32.000 31.000 30.000 26000 29.000 30.000 29.000 30.000 32.000 1000 27.000 25.000 29.000 23.000 23.000 27 000 27.000 21.000 29.000 27.000 1200 13.000 10000 I1.000 17.000 21.000 15.000 3000 15000 12.000 13.000 1400 26.000 6000 12.000 I4.000 11.000 11000 0000 9.000 0.000 6.000 Theeftrnu Ustmatstoed Rtak -Tlated Isetank Come-3t L Me.a N-MS.A Man Min MAX CV% N Sozu CyIddl Meo N-Men D-C=01 31.100 1.0000 31.100 21.000 33.000 7.132 1o 31.100 1.0000 600 30.300 0.9743 30.300 21.000 33.000 5.401 10 95.00 75.00 30.300 0.9743 Soo 29.600 0.9513 29.600 26.000 32.000 5.736 10 37.50 75.00 29.600 0.9510
*1000 26.000 0.1617 26.800 21.000 29.000 1.760 10 60.00 73.00 26.100 0.3617 11200 14.000 40.739 14.100 2o.0oo 21.000 22924 10 3300 73.00 14.000 0.4739 21400 :.S00 0.2733 3.500 0.000 16.000 64.259 10 35.00 7S.00 3.500 0-2733 AnxlIary Tests Statistic Critical Skew Kert KoknohorDTat icatea mealdsntbtion(p>0.01) 0.99586133 1.033 .0.4676473 1.52342436 Bartt Tett sdiCtat tutequal v e (p - 1.1511-3) 20.20035U4 1.0862722 llypthesis Test (-lI. 0.5S) NOWC LOAC CV T]
Ste, Many-On. Rank Test 300 1000 . 894.427191 Treatmets w I trol Uesar lsterpebtloe (200 Rosamples) P.1st ting/L SD 9b% CL Skew ICOS 303.92357 125.04005 440.176515 398.220443 -1.1130 ICIO 913 53.0220931 113.34328 2003.03634 0.3160 ICIS 1006.08333 29.7439229 923.373336 1032.54913 *1.2221 1.0 I0 2032 13.1763532 1000.23143 1057.05037 -0.5002 0.9 IC25 1057.91667 13.9219323 .1029.73462 10337967 .0.2323 IC40 1135.66667 14.3046107 1108.67963 1164.35622 0.1112 0.3 Icso 3137.5 18.1570691 113d.93744 1232.04375 0.5453 0.7 0.6 C 10.5 0.4 0.3 0.2 0.1 0.0 0 500 1000 1500 Dos* agliL De-Respenee ,lMt 07-12-05new
Environmental Testing Solutions, Inc. Statistical Analyses Cerfodaphala, Survival and Reproduction Test-Repoduction Sart Date: 7112r205 Test ID: Cd&SC1CR Sanpile ID: REF-RefToxicant End Date: 7/19/2DOS Lab D: ETS-Eavir. Teting SaL Sample Type: NACL-Sodiun dkcxide Sacle Date: Prctocol: FWCiR-EPA821.R.02-013 W Test Species: CD-Ccriodsphnis dubis Coae-mg/L 1 2 3 4 5 6 7 a 9 10 D-CcAtrO1 28.000 29.000 35.000 320D0 30.l00 30.000 33.000 32000 33.000 29.000 600 31.000 28.000 31.000 31.000 33.)00 29.000 32000 28.000 30.000 30.000 BOO 28.000 32000 31.000 30.000 26.000 29.000 30.000 29.000 30.000 31.000 1000 27.000 25.000 29.000 28.000 28.000 27.000 27.000 21.000 29.000 27.000 1200 18.000 10.000 11.000 17.000 21.000 15.000 15.000 16.000 12.000 13.000 1400 16.000 6.000 12000 14.000 I1.O0 11.000 0.000 9.000 0.000 6.000 Tmansfoar: Untrmanormed I-Tailed Come-mg/L Mean W-Mean Mean Mi Max CV% N t-stat Critical MSD D.Ccitrol 31.100 1.0000 31.100 28.000 35.000 7.182 10 600 30.300 0.9743 30.300 2S.000 33.000 5.401 10 0.578 2287 3.165 800 29.600 0.9513 29.600 26.000 32000 5.786 10 1.084 .287 3.165
*1000 26.800 0.8617 26.800 21.000 29.000 8.760 10 3.107 2.257 3.165 *1200 14.800 0.4759 14.100 10.000 21.000 2Z924 10 11.778 Z27 3.165 *1400 8.500 0.2733 8.500 0.000 16.000 64.259 10 16.331 Z2Z7 3.165 AniliaryTests Statistic Critical Skew ICrt KildmbxaoD Test indicates narnal distribuiticn (p > 0.01) 0.99586135 1.035 40.46764754 1.52342436 Brtletts Teat indicates anequal vmriuw (p - 1.15E43) 20.2003574 15.0862722 Hypothesis Teat (I-lI, 0.05) NOEC LOEC CkV TU MSDu MSDp MSB MEE F-Pmb df DioEtfaTesa Soo 1000 t94.427191 3.16452289 0.10175315 905.576667 9.57592593 1.9B-25 5.54 Treatments vs D-Ccitrol 07-12-O5rew
Environmental Testi-ng Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 IJ AL6Ae. Species: Ceriodaphniadubia CdL-aCLCR #: Z38 Daily Chemistry: Dav _~~Analyst VJE%5__Y 1 Concentration Parameter pHI (S.U.) -1.9\ SB -l 5O §lR~ DO (mg/L) 8. c I.6 8.O . .O CONTROL Conductivity (pzmhos/cmn) 0 Alkalinity (mg CaCOlL) Hardness (mg CaCQ 3/L) Temperature _____(°C) 24.(S 2tl,A 2L .1 ZS .O 'v4A. uoeq.5 pH (S.U.) -1:8S? t#0)q) DO(mg/L) -. __ .'i __0 _ g.!5 600'mg NaC/L Conductivity (jPmhos/cm) __ _ __ _4__ _ __ __ _ _ Temperature 2S.2... 2el .S pH (S.U.) 1.79 _.? _____2_ DO (mg/L) ____ R1_ _ _11l 800 mg NaCVL Conductivity (pimhos/cM)__ _ _ __ _ _ _ Temperature ?A2-7 2q.1 7. $ (°C) b6 __ _*° pH(S.U.) 19 32 2-85 o t.F _____ DO (mg/L) __ 0__ l __ ___ l 1000 mg NaCltL Conductivity 33C - 2Z<=0 Z2 - (gmhosfcm-) _ _ _ _ _ _ Temperature 24. q . 2 2S. I .24.1 Z41 __ __ _ __ (C) _____'I
~~-pH(S.U.) 1.'11 2 -____~~
4?.. -9 tZ DO (mglL) l. 53 l . 6-)Xt.1 -l.9 8- O 4.%o 1200 mg NaCllL Conductivity 21(cO Z&01- zc eo tpmhos/cm) _ _ _ _ ______ Temperature Zq. q7 CC7 (°_) _lA _S -2q._I 2Y1 - Z pH (S.U.) U.1i 3.3-0 -- 4q DO (mglL) l.95 -W I i -. I A-I. 7 - . 1400 mg NaCIJL Conductivity 3SO0Z520CIM Igmhos/cm) _____ Temperature 72q. S 274 . 2q. 7. q.S (°C) I
%1l.1 $ _rom STOCK Conductivity I (,mhoslcm) 136oD J - IJ- I -
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Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphniadubia IlA3 IL5Ae. CdNaCLCR #: :: Analyst I. I t 3 a IJA0 I I Day 5 t1rD 1 l lt-rl° 6_ 11 aZV I I Concentration Parameter nH (S.U.) r_ \_._, . . . I -7.G g . 1 1h 9 1 qi.2 1 7.71 1 -7.Ba- 1 DO (mg/L) &O 1 I 411 1-4 III
~~]7.7 2 1 _~~
1u; CONTROL Conductivity 3i5 (gmhos/cm) 301 W;o&I 38c Alkalinity (mg CaCOAL) Hardness 8 2- &(0 (mg CaCO3/L) Temperature (°C) I 7ZS A VA A -24A 2zq W-1 un 24.9 pH _S.U . It. DO (msz/) 'I n.17i r 7.9A %7.qs e 600 mg NaCUL Conductivity jq57 (i4mhos/cm) MILO___ Temperature ?A. 5 2ql. Z - bzA Z. A-. pH (S.U.) ISIg f.°
*'IC) 7.AA. -/A0 T laz. r-g2 -7.7 1 A= -7.?,ZG DO (mgtL) e D1.7 o 7.7 1.
800 mg NaCVIL Conductivity i __1_l Temperature Z0 z 7l L 24G.E P z7a _ _ __ _ __ _ _ _ _ (0C
~~.pH (S.1 T~~
~~a 77 71 7U..~~
DO(gL) _ l1; n. - 7- 7 .il 1000 mg NaCVL Conductivity l240 2z O (.unh s/cm) _____0 _____ ______ Temperature 21-1 ZS.1 Z . .' ZLI. 24 .9 _ __ __ _ _ _ _ ( 0 c) pH (S.U.) ?.N $ t xff7 .! 7< 1 ;7 7 DO (mg/L) e. .7 1.1 0 1 -. ° I7. 1200 mg NaCLL Conductivity Trathosucm) _b.A_ 2(O-0ZZoA_
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_ _ __ _ _ _ ( 0C) _ _ _ _ _ _ _ _ _ nIT tS.TUA _ _ _P. ls 7-r hn -f1\2 J -- I_- . I t P
-7 1I 4I N -1. ; ZRL V- k __ j DO (m-/LI 810 i -,q I 117 1.7 R. I -7.9 WR1 1 1400 mg.NaCVL - - l -
Conductivity 30q0 (plmh s/cm) I 8000 U-.---, (on 2, VC l ag auso Ark Temperature (0 0C 2q.1 zS.O I 2'q. G -ST. I N4.- S
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2L1.9 Z' It* I _ _ . STYUCK Conductivity (umhosfcrnM ,T. . - a '- -- -- ---- ' - 91 Initiail
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PERMITTEE NAME/ADDRESS (Include FacilityName/locatlon iDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT________ DISCHARGE MONITORING REPORT (OMR) D04 (SUBR 01) OMB No. 2040-01 Address P.O. BOX 2000 - - - - __ TN0026450 l 101 Y I F - FINAL SODDY. DAIY__ TN 37384 _. _. _ I PERMIT NUMBER I DISCHARGE NUMBERl ANNUAL MONITORING (PCBS) ady_ TVA- SEQUOYAH NUCLEAR PLANT __ Location HAMILTON COUNTY __________ EFFLUENT o R MO DAY ToYEAR I MO ATTN: Stephanie A. Howard From 04 108 I 01 ITo 05 l 07 31
*** NO DISCHARGE D -
NOTE: Read Instructions bfore comnletin this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE ________ ____________EX _______ OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS POLYCHLORINATED BIPHENYLS SAMPLE T <0.0 19 0 1/365 GRAB MEASUREMENT 39521 1 0 0 .;,,PERMITI ee*ANNUA':***I *e** MGL~GRAB-. I*e**'.
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~REQUIREMENT' ~ :'2~~A MEASUREMENT EPAorm332-1 REV I~9 Prvi~SAeiiosmayLe ue ae1o NAMEtlTnLE PRINCIPAL EXECUTIVE OFFICER I Certify trider penalty df law that this doocznesrfand allttahments wer prtepifed unidf my * . // TELEPHONE DATE J.RadyDttction or supenrsion inaccordance wth a systemt desIgned to east,, Ihat qualdted persomrel / -Z }2 I4I_
J. Rndy ouetproperly gather and evaluate the normthion submitted. Based onmy Iquiry oftlheperson or M6t. _ peronswhomangehsysem orthosepersonstdirectlynsponsibeogallheringthe Principal Environmental Engineer 423 84346700 05 08 l10 Slte acePedntscurate. end complete. I am aware thattere are sig~nifican penaltis for submittIng false SIGNATURE OF PRINCIPAL EXECUTIVEi TYEDO PITEformatton.insiuding the possioliiiY oiiine and Irrprtsorsrenri or lnowniolsatlons. OFFICER OR AUTHORIZED AGENT COOE NUBE YEAR MO lDAY COMMENTS AND EXPLANATIgON OF ANY VIOLATiONS (Referenc e all attachments here) F-FA FOrM 3320-1 (REV 3199) Previousecritionsmay be used Page 1 of I
PERMITTEE NAMEIADDRESS (IncludeFac7iityName/tocatIon IfDifferent NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT - - - - DISCHARGE MONITORING REPORT MDMR) (SUBR 01) 0MB 4o. 2040-0004 Address P.O. BOX 2000 _____ _____ __ _ _1NTEROFCE SB- - - - - - - - - - I TN0O60 103LG F - FINAL SODDY - DAISY JN 37384 _______ PERMIT NUMBER I DISCHARGE NUMBER LOW VOL WASTE TREATMENT POND Fad!y 'VA SEOUOYAH NUCLEAR PLANT _ _ Location _AMITNqCOUNT?.._._.. IONITQRINCEFRI IEFFLUENT IYEARI MO - II MO I DAY In .n. i ... ATTN: Stephanie A. Howard From [-05 l 07 I 0o-1 To I 05 1 07 l 31 1 -- NT. Ul0%RadIntr b r m n NOTE: Read instnictons before compfetinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ __ _ EX o f: TY PE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ** 6.8 8.7 12 0 13131 GRAB MEASUREMENT 00400 1 0 0 PE M . e * 'O 6W0L Y LB MTHREE BY G:RAB EFFLUENT GROSS VALUE REUR M N M N M Mu~MA I U WE SOLIDS, TOTAL SUSPENDED SAMPLE 103 118 26 12 13 19 0 4131 GRAB 00 5 E M T-j ~~ 10 ~ 5 ~ 7 - LBSIDY MGL . WEEKLY, GRAB, MEASUREMENT EFFLUENT GROSS VALUE f M AV D I Y X I.. M AV % D I Y M 2 , > 2 TREATMENTPLANTMEASUREMENT ',EQUIREMENt:AE 45. . 1 2- 00 . - '
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PERMITTEE NAMEIADDRESS (Include Facilitv Name/locatlon if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR01) OMB No. 2040-0004 Address P.O. BOX 2000 l - - i _ _ ___(I __ EROFFIEES-EB _-_ _ -)_- __-F- __
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j1 107 G F- FINAL SODDY - AISY TN 37384 I PERMIT NI FaqlL TVA- SEQUOYAH NUCLEAR PLANT I I DISCHARGE NUMBER I METAL CLEANING WASTE POND Location HAMILTON COUNTY MOlNITORlING PEpIODl I EFFLUENT ATTN: Stephanie A. Howard YEAR MO From I05 107 l 01 I ToI 05 107 l 31 I DAY I MO DAY
-* NO DISCHARGE [3] ***
NOTE: Read Instructions before comnletino this form. PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE ______________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 12 00400 1 0 0 PEQR4MIT> . ._.
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MEASUREMENT 19 00556 1 0 0 r '-N MG ,** ** ., ... MPDS EFFLUENT GROSS VALUE k ~.-*. ___ _____ AL X ___
COPPER, TOTAL (AS CU) SAMPLE ******** 19 MEASUREMENT P9 01042 1 0 0 P.-MGIL g M, AILY COMPOS EFFLUENT GROSS VALUE
',REQiJIREME1 ~ -~ ' '~ -. ~,w~r DALY IRON. TOTAL (AS FE) SAMPLE *ee 1 MEASUREMENT 1 01045 1 0 0 iPERMIT-..~ - .. ~ ae*. 1-EFFLUENT GROSS VALUE REUIEEN4 .DAIL1TO ;. GI DILMXMO FLOW, IN CONDUIT OR THRU SAMPLE ****
TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 ~ ':PRI,~"EORT,; ~- PORT-'I' MGD 7 "DAILY-' CALCTD' EFFLUENT GROSS VALUE REQUIRENj
.- AMAG ALYMX -.. .. .- y '
___'h,___ __ __ NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify uwider perialty of low that thisdowmener and all attscments were prepared under my dil ~TELEPHONE DATE directon or supervisIon In accordance wlth a system designed to assure that qualified personnel J c_ J. Randy Douet proierly gather and evaluate the Information submitted. Based on my hquIry of the person or persons whomanagethe ystenoirtho@epersonscbtr renporsl e forgatheringthe Principal Environmental Engineer 423 843-700 05 08 10 StViePedetInformatin, SPacrate, andthecomplete. Information I amsubmitted aware thaIs therUe
~~to ftharebest of my Inowledge sIgnifIcant penalties and bellef, true.false for submitting SIGNATURE OF PRINCIPAL EXECUTIVE 423__843__6700___________~~
TPED OR PRINTED nformation, Icltding the possibility of trie and Imprisornment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference all attachments here) No Discharge this Period Preiou edtin may use . Page.. 1_ 1 of__ U-A t'ot LI' JJZU-11 (H* I-Onn JiZ- (KF-V Ae V JIM 3199) Prewous ecfMons may be USed Page 1 of I
PERMITTEE NAMEJADDRESS (tncfude FacilityName/Location fDifferent) NATIONAL POLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name __TA - SEQUOYAH NUCLEAR PLANT __ DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 ____LNF~tOECESB-- - - - - I TN0026450 110 G I F-FINAL SODDY - DAISY TN 37384 I PERMIT NUMBER IDISCHARGE NUMBER IRECYCLED COOLING WATER djy__ TA-SEQUOYAHNUCLUEAR LANT7 Location _AMILTON COUNTY __________ MONITORINGQ PFRIOD I EFFLUENT Froml II YEAR I MO I DAY
` NO DISCHARGE F1 *1 ATTN: Stephanie A. Howard From 05 0 l01lTo I05 1 07 l 31l NOTE: Read Instrucons before completing this form.
PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE __ _ __ _ _ _ _ _ __ _ _ __ _ _ __ _ __ _ _ EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ******** 12 MEASUREMENT 00400 1 0 0 * ; . . u SuU ,DAILY ,-GRABL, EFFLUENT GROSS VALUE EFFLUENT GROSSMVA-UE
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01042 1 0 0 .PRE0IRE~ H '-- _aeae'~ .r MGIL*e~.. _~* m COMPOS~eae 01045 TOTAL IRON, 1 0(AS 0FE) -~PER SAMPLE rZ:f *
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TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 ;-' R ORT - MGD . n rCALCTD _ EFFLUENT GROSS VALUEREURMN _____ - ____ NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certity under penalty of law that this documernt and alnattadrients were prepared under my direction or supervision In accordance with a system designad to asswa tha"t qaiifd prsonnell
.* / ) d/ TELEPHONE DATE J. Randy Douet ipropery gther and evalue the Iformation submitted Based on my inquiryof the person or '-5 Sipersons who manae the system or those persons desdl responsible for gat the Principal Environmental Engineer 423 843-6700 05 08 10 S__e Vice President accuate. and CompIete. I am eware that thare are significent penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED 1 T Pnaflonn Including t possnbnnydfine and Irisonment foraknowingfviolationte OFFICER OR AUTHORIZED AGENT ARt-A INUMBER CODE YEAR MO DAY COMMENTS AND EXPLANATION OF ANY ViOLATiONS (Reference affattachmentshere)
No Discharge this Period EPA FOrM 3320-1 (REV 3199) Prevtous efforts maybe used Page 1of I
PERMITTEE NAMEIADDRESS (Include FacilityName/LocationifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT ____ DISCHARGE MONITORING REPORT (DMR) OMB No. 2040-0004 (SUBR 01) Address P.O. BOX 2000 - - __ _ _ _ _LJNTEROFfCE SBMM . . . . . . . . _ l TN026450 l 116 G F-FINAL SODDY DAISY_ TN 37384 _______ l PERMITNUMBER 1D=ISCHARGENUMBER BACKWASH FadjN_ TVA - SEQUOYAH NUCLEAR PLANT _ Locca.on _AMILTON COUNTY __________ I EONITLQRING PER1OU l EFFLUENT YEARI M+/- A TO YEAR I MO DAY NO DISCHARGE = - ATTN: Stephanie A. Howard From 05 i 107 l °01<-1 To05 l 07 31 NOTE: Read Instructions before ComDietna this form. PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE __ _ __ _ _ _ _ _ __ _ _EX OF I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1131 VISUAL MEASUREMENT 01345 1 0 0 PERMIT; ....... .. .. ' REPORTi. PASSX m mSEE ISUAL EFFLUENT GROSS VALUE OEQUIEMENT
~~?ROURMET ;ILR>I' ~ FA OIL AND GREASE VISUAL __ ____ _ _ ____ __ ___~~
SAMPLE __ __ __ _ __ __ _ __ 0 94 M ',T O0TA L ,' FI I 0< PE M ITr 1131 ~VISUAL MEASUREMENT 84066 1 0 0 -; PERMTORT YES=1 Ei R M'-IT SEE -'VISUAL, EFFLUENT GROSS VALUE RURMET OTTA" NO=0 -'" ____PRI . M;EASUREMENiT-SAMPLE MEASUREMENT x REQUIREMENT,'i 7. ..... Hi - .- ,.*: _ SAMPLE MEASUREMENT UIREMENe. R 5Q, SAMPLE MEASUREMENT
*RiEQUIREMENT~ '7'~ ~ *~:
3~~ SAMPLE ____ MEASUREMENT SAMPLE MEASUREMENT
*EUIREMEN1 '.- '*r ', .
e,4 Ca NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this docznent and an attEdcments we prepared under my TELEPHONE direction or supervision In accordance with a syslem designed to assure that qualifed personnel 1 //.. ' V d DATE J. Randy Douet properly gather and evaluate the Itformtallon submitted. Based on my hiquiry of the person or / Io persona who manag tft system, or those persona directly responsible for gatherkng ft Principal Environmental Engineer 423 843-6700 05 08 10 Site Vice Pesident ltfOI7T~b~n*the Inometlon submitled Is. to fte best of my knowedge end belier, true, _____________I eacrale, and complete. I am aware that there are slgnificant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TYPED OR PRINTED TEORmPtion, hIclidng the pssiblitly of fine and Imprisnment for knowing violations.
___________________________________________CODE OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attachments here) Operations performs visual Inspections for floating debris and oil and grease during all backwashes. ~rP. Drkm EPAru0 .53eU* tKCV Knee 3J=Us1I 4r~ i-"evs A.._AXilos may a..... iS__ 319S) 1-mvious edirlons may De used Page 1of 1
PERMITTEE NAME/ADDRESS (Incrude Facl7ity Name/tocatin IfDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name _ TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No. 204040004 (SUBR 01). Address P.O. BOX 2000 _ _- £EROFFIE .SB-2)__________ l TN0026450 1 117 G F - FINAL SODDY- DAISY TN 37384 I PERMIT NUMBER I I DISCHARGE NUMBER BACKWASH Lacy_ WA SEOUOYAH NUCLER PLANT Location HAMILTON COUNTY _________ MONIT o vER10 EFFLUENT ATTN: Stephanie A. Howard From 05 YEARIMO Osl 07 l 01 I DAY IYEAR IMO ToI 05 107 l 31 i DAY
~~-* NO DISCHARGE W "-~~
NOTE: Read Instructions before comlletrin this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE ____________ _______ _______ ____EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE *.**** * ****** 0 9A 0 1 31 VISUAL MEASUREMENT 01345 1 0 0 .. RMO R ORTA PASSO ._. 0f a'EE VISUAL: EFFLUENT GROSS VALUE q. ~ AOTTAL-'FI1
.70____ 'Y PRI OIL AND GREASE VISUAL SAMPLE 0 9****°4 *
0 1 /31 VISUAL MEASUREMENT 84066 1 0 0 ~ PERMI~t`'-~-~ ~RPRp E= ' ' ~SE VISUALr MEASUREMENT MEASUREMENT _
EFRLUENTEGROSSV.A.LUE QTAC 'PRMT SAMPLE MEASUREMENT L ~W~ R.* -. SAMPLE MEASUREMENT. MEASUREMENT SAMPLE
~~'REQUIREMENT REQUIREMENT j - P .:E :'. *; , * ,'-&"- - . ...~~
NAlifErITTLE PRINCIPAL EXECUTIVE OFFICER I Certify wider penalty of lawvthat thIs doaznent and all attsclwents wers prepsfed unider mny EPAJ.(EV Randy Douet orm33201 399) Site Vlice President dirertion or supervisnion e, PrevouseditonsmabENuedPae-'o operotyat8her ccordane with a system desugned to assure that qulified personnel snd evaluate the Wnormstbon pebrsonwthemanage aruae an submitted. Based on my Inuwy fd the persori or thesys~tem is t h bnsdtrectly responible horgatherh9g the ea Ispif tha
~~ther bres of myan pedgtes hr~ ubmflri iae At~~
~~.I t , ,/J~~
~~({'4Y Principal Environmental Engineer SIGNATURE OF PRINCIPAL EXECUTIVE~~
)
d I 423 TELEPHONE 843.5700 05 DATE II _t.L 08 10 rmand OFFICER OR AUTHORIZED AGENT l UER YEAR MO DAY COMMENTS AND EXPLANATtON OF ANY VIOLATIONS (Refference aDl attachments here) Operations performns visual inspections for floating debris and oil and grease during all backwashes. EPA Form 3320-1 (REV 3199) Previous editions may be Used Page I of 1
PERMITTEE NAME/ADDRESS (Include FadlityName/locatlon IfDfferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000
- - -_( 1,flER 0FFCE- EQ -: -B -- -- - - - - - - l TNO4 5O 118 F-F - FINAL SODDY - DAISY TN 37384 _______ I PERMIT NUMBER DISCHARGE NU BER WASTEWATER & STORM WATER Fadi_ TVA- SEOUOYAH NUCLEAR PLANT ___
Location HAMILTON COUNTY __________ I MONITQRINC' PFRIQQ 1 EFFLUENT YEAR I MO I DAY IMO I DAY ATrN: Stephanie A. Howard From I05 I07 l 01- Tol 05 107 l 311 ** NO DISCHARGE [ *** NOTE: Read Instructions before completing this form. PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREOUENCY SAMPLE ______________________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE *
19 MEASUREMENT III 00300 1 0 0 by*4 PERMS. , d.;0?w *Wi:A -
EFFLUENT GROSS VALUE FEUIEMNT Zlr DAIL;Y MILMTIE/
.: .-.. . RA SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 0 J;fr"PERMI'~~'****.~****:~ ****f.' i -"10 MGIL ~ fN / GA REQUIREMET '-
~~E F F L U E N T G RO S S V AL U E ,,- : A e e. _ _ _ _~~
~~__ __ __ __ __ . K.~~
SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 0 PERMIT.'..e; i.L**--a----. dO MLIL ON N , -GRABH.
'REfQUIREMENT-- *NTH:',%',\-.-'DAL DALY Mn EFFLUENT GROSS VALUE t.MOTHs IN' FLOW, IN CONDUIT OR THRU SAMPLE 03 **
TREATMENT PLANT MEASUREMENT 50050 1 0 0 PR, -V,,', P Rr- ,.R-EP MGD Ax *.ONCE/, 'ESTIMA; SAMPLE_ MEASUREMENT T ;;REOT -,(,,; SAMPLE MEASUREMENT SAMPLE MEASUREMENT SA~Y~
~~'RE0UIREMENT. i , . . - -~'*.. "~~~
NAME/TTLE PRINCIPAL EXECUTIVE OFFICER I Certify tader penalty of law that this Panzwrt and all attacints wexe prepared taider myr /' TELEPHONE . DATE hdireton or aupervislon hi accordacnce witlh a system designed to Basuns that quaslified personnel ~j7'M.jjp i~* (jV1OVCA i J. Randy Douet properly gather and evaluate the Information sutbmtted Based on my Inquiry of the person or _ A.,~
idpersonswhomsnagethesytemothiosetptsotn'sdlratyirX~lfesoendstiblefrgthethPrlncdpal Environmental Engineer 423 843-6700 05 08 10 Ste Vice President s acanate, and complete. I emra ware that there are siganificant pvenalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, Inrudingtheposstbllityof fine and tmiortsomrrentfor~ioowingviotatlons. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _CODE COMMENTS EAFr3301(E399 AND EXPLANATION OF ANY VIOLARIONS (Reference Prvoseitrmaions usedto thye aftattachments umttdI.t here) ebs o ykoldead eif re 2 Page70 15of 1 During this reportng period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EPA Form 3320-1 (REV 3199) Previous edfflons may be used Page 1 of 1}}

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