ML052270384

Discharge Monitoring Report for July 2005

ML052270384
Person / Time
Site:	Sequoyah
Issue date:	08/11/2005
From:	Howard S Tennessee Valley Authority
To:	Hannah C Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation, Div of Water Pollution Control
References
Download: ML052270384 (110)

Text

{{#Wiki_filter:Tennessee Valley Authority, Post Office Box 2000, Soddy-Daisy, Tennessee 37384-2000 August 11,2005 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6t" Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Mr. Chip Hannah

Dear Mr. Hannah:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JULY 2005 Enclosed is the July 2005 Discharge Monitoring Report for Sequoyah Nuclear Plant. Please contact me at (423) 843-6700 if you have any questions or comments. Sincerely, Stephanie A. Howard Principal Environmental Engineer Signatory Authority for J. Randy Douet Site Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure): Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue A Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 I'Ed on reyldpa

PERMITTEE NAME/ADDRESS (Include Fadity Name/locaton if Different) tame TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _ _ _ _1tfTEjOFFICE aN-2T ____A(LD~YO _ DAISY_ TN 37384 FaWy_ TVA,SEQUOYAH NUCLEAR PLANT Location _AMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0026450 101 G lF - FINAL I PERMIT NUMBER I DISCHARGE NUMBER DIFFUSER DISCHARGE Form Approved. OMB No. 2040-0004 I MONITORING PERIOD --- I EFFLUENT l fi -l M e-6 l I YEAR JMO IDAY-I I YEAR I MO DAY ATTN: Stephanie A. Howard FromI 05 1 07 1 01 I Tol 05 1 07 131 1 NO DISCHARGE a NOTE: Read Instructions before completina this fomn. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE _EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE 30.0 04 0 31 131 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 0 EMT

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Q Site Vice residento manage the system, or those persons dilrectly responsible for gathering the Principal Environmental Engineer 42 83670 5

08 1 SiteVic Prsidntacoxate, and complete. I am a*wae that thee ame signIficanit penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVEI TYPD RPINEDInformation, irduding Mhe possibility of fine an Imrsomn fo kr~n vIlto OFFICER OR AUTHORIZED AGENT AREA INUMBER

• YEAR MO DAY TYEDO PITEI I_________________________J______________

CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Referenceaeliaefachmrrents here) No Closed mode operation. CCW data for July 2005 Is attached. Veliger monitoring Information Is attached. CrA ro0 J3ZU-1 tKtV 3XS-) f-MVIOUS MITI= May De usea Page I of 2

CCW TRENCH Date/Time Collected Extractable Petroleum Hydrocarbons Analysis Date/Time Analyst Method 07/01/2005 0720 <0.5 mg/L 07/06/2005 2317 JBR EPA 8015B CCW CHANNEL Date/Time Collected Extractable Petroleum Hydrocarbons Analysis DateMme Analyst Method 07/01/2005 0715 <0.5 mg/L 07/06/2005 2239 JBR EPA8015B

Sequoyah Inplant Sampling Mean# of Water Mean#of Water SAMPLE Sample Date ZM~mt % Settlers Temp. Sample Date Asiatic Temp. LOCATION SUB LOCATION COLLECTED BY ZflI(,C) Clamslm CC) 04101/2005 04/06/2005 04/08/2005 04/13/2005 04/15/2005 06/23/2005 06/24/2005 06/29/2005 07/01/2005 07/06/2005 07/08/2005 07/13/2005 07/15/2005 07/20/2005 07/22/2005 07/27/2005 07/28/2005 07/29/2005 08/03/2005 0 0 0 0 0 432 162 290 318 0 122 51 0 38 0 143 488 90 0 0 0 0 0 0 50 80 80 100 0 29 33 0 50 0 100 100 20 0 14 13 14 16 16 25 25 26 27 26 26 26 26 26 27 27 27 27 27 04/01/2005 04/06/2005 04/08/2005 04/13/2005 04/15/2005 06/23/2005 06/24/2005 06/29/2005 07/01/2005 07/0612005 07/08/2005 07/13/2005 07/15/2005 07/20/2005 07/22/2005 07/27/2005 07/28/2005 07/2912005 08/03/2005 29 81 54 106 0 154 30 168 127 677 1132 998 186 303 100 341 627 663 500 14 13 14 16 16 25 25 26 27 26 26 26 26 26 27 27 27 27 27 INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT INPLANT Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water Inplant Raw Water QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT QUANT Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock Dick Adcock

quoyah Forebay Sampling Ma ofWater Mean# of Water NOTES: % SB SML mple Date Mean#of % Settlers Temp. Sample Date Asiatic Temp. Gravid LOCATION SUB TYPE COLLECTED BY zMm 3 Asiatic LOCATION TYPE C)Casr 3 C) Clam 116/2005 123/2005 128/2005

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/1 5/2005 1/21/2005 1/29/2005 1/05/2005 1107/2005 i/04/2005

/09/2005

/12/2005 i/17/2005 il19/2005 3/23/2005 5/26/2005 3/30/2005 3102/2005 3/07/2005 3/09/2005 3/13/2005 3/15/2005 3/23/2005 i/24/2005 3/27/2005 D/30/2005 7/25/2005 7/28/2005 8/01/2005 0

0 0 0 0 0 0 0 0 0 0 0 43 31 579 242 1530 1344 240 192 236 78 0 0 0 0 24 0 28 0 0 0 0 0 0 0 0 0 0 0 0 50 50 50 50 26 30 40 40 42 33 0 0 0 0 100 0 100 7.9 02/16/2005 0 8 02/23/2005 0 7 02/28/2005 54 7 03/07/2005 82 10 03/15/2005 122 10 03/21/2005 105 12 03/29/2005 101 13 04/05/2005 525 14 04/07/2005 252 17 05/04/2005 0 18 05/09/2005 632 18 05/12/2005 549 23 05/17/2005 106 21 05/19/2005 299 20 05/23/2005 22 20 05/26/2005 61 22 05/30/2005 1831 22 06/0212005 1989 23 06/07/2005 613 24 06/09/2005 425 24 06/13/2005 928 24.5 06/15/2005 2491 24 06/23/2005 171 24 06/24/2005 784 24 06/27/2005 305 24 06/30/2005 0 27 07/25/2005 1205 27 07/28/2005 0 28 08/01/2005 1505 7.9 8 7 7 10 10 12 13 14 17 18 18 23 21 20 20 22 22 23 24 24 24.5 24 24 24 24 27 27 28 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 0 Outside Plant Forebay Quant S Jones-Brier, J Justice 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe 0 Outside Plant Forebay Quant S Jones-Brier, Chris Coe,JRigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 60% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant J Rigsby, M MacCarroll 33% Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 0% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll 66% Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby, MacCarroll, Shaffer 0 Outside Plant Forebay Quant J Rigsby, M MacCarroll 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant W. Allen 0 Outside Plant Forebay Quant W. Allen 0 Outside Plant Forebay Quant W. Allen 0 Outside Plant Forebay Quant W. Allen 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby, M Wallus, G Shaffer, M l 0 Outside Plant Forebay Quant S Jones-Brier, J Rigsby

PERMITTEE NAMEIADDRESS (Include Facility Namel.ocation ifDifferent) Na TVA - SEQUOYAH NUCLEAR PLANT Address P BOX 2000 ___L[NTEIO OMIE SIO2) SODDY-DAISY TN 37384 Fdjy. VA-SEQUOYAH NUCLEAR LNT____ Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0025 101 G F - FINAL I PERMIT NUMBER DISCHARGE NUMBE DIFFUSER DISCHARGE Form Approved. OMB No. 2040-0004 ftnNmTrnRlimr. PFRIODf I EFFLUENT I IylV,l,wV,llrV F-lRlVy E m05 1 07 l 01 T o 05 07 1 31 l NO DISCHARGE m NOTE: Read Instructions before completing this form. NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this dowrtent and anl attadyents were prepared under my Al1t TELEPHONE DATE drectionorsupervisioninaccodancewithasystemdesignedtoasse thatfiualiaedpersonnel (j( 9A[.di J. Randy Douet properly ather and evaluate the Information submitted. Bssed on rmy Inquiry of the person or .- t v. persons who manage the system, or those persons directly responsible forgathering the Principal Environmental Engineer Inforrmation, the Inrormeton submitted Is. to Om best of my iedge and belief, true, OF423 843P700 05 08 10 Ste Vice President crate, and complete. I em ewamr that there we significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTiVE TYPEDORPRINTED information. Inckiding tha possibility ofine and priornent for knowing violtions. OFFICER OR AUTHiORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY ViOLATIONS (Reference aff attachments here) The following Injections occured: 1. PCL-222 Copolymer (max. calc. conc. was 0.019mgL-Iimit 0.2mg/L) 2. PCL-222 Phosphate (max. cale. conc. was 0.054mg/L-limit 0.2mgQL) 3. Blodetergent 73551 (max. calc. conc. was 0.014mgQl-lmit 2.0mgQL) 4. H-150M (max calc. conc. was 0.0369mg/L-limit 0.050mgQL) 5. H-150M (low detection level analytical method was <0.020mg/L-limit 0.050mglL) EPA orm33201 (EV 199) Pre~ousediion maybe sed ageof EPA Form 3320-1 (REV W99) Previous edillons may be used Page 2 oF 2

PERMITTEE NAME/ADDRESS (Include Facilit NameltocatIon IfDifferent) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ___I qNTEROFC M SB-,ZN. SODDY - DAISY TN 37384 Fadly VA.SEQUOYAHN NCLEARA PLNT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR For DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OM I TN0026450 101 T F-FINAL PERMIT NUMBER [ ! ISARGE NUMBER BIOMONITORING FOR OUTFALL 101 IMONITORING PERIOD I EFFLUENT IYEAR I -MO I DAY lI YEAR I MO I --DAY Il-* rkn E.g-L A r rm Approved. B No. 2040-0004 ATTN: Stephanie A. Howard Froml 05 1 07 1 01 I Tolo5 0 07 1 31 1 NOTE: Read InUsructo o Lo NOTE: Read Instructions before comptetinq^ this fhmm NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Csrtfy under penalty of law thal ths document and eti atladvnentas were praepard under my A TELEPHONE DATE direction or supervison hI accordance with a system designed to ass"re that quaruied personnel (,.4,rUiIte. Q

• Y persons who manaes the system, or those persons drwecty responsible for gathering the Principal Environmental Engineer Sit Vi PeIdet ifomabon.the Itlormatlon submitted Is.

the best of my knoledge and belief. true. ______423 843_6700 05 08 1_0 e Me resen orate, and comptete. am aware that there are significant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE INformation, hIcuding the posshility of fine and hIprisonment for knowhg violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED I ________________________________________________CODE COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference all attachments here) Toxicity was sampled July 17-22, 2005. Report Is attached. r-rvru earn mayoe usei Pag 1f of 1^snX.__:__. tPA Forr^ 33Z2t-7 (RtV;3f99) 1-mmfius eamions maybe used Page 1 of I

August 9, 2005 Ruth Ann Hurt, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, OUTFALL 101, JULY, 2005 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report for your records. The report provides results of compliance testing using fathead minnows and daphnids. Outfall 101 samples collected July 17-22, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values for both species were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. In addition to the routine compliance test, fathead minnows were also tested in Outfall 1.01 and intake samples which were treated using UV exposure for pathogen removal prior to introduction of test organisms. Fish pathogens present in intake water have been the suspected cause of anomalous dose responses and high variability among replicates in previous toxicity testing at Sequoyah. At the time this study was conducted, mortality was not demonstrated in minnows exposed to routine compliance samples. Reduction in growth was statistically significant in UV-treated intake samples. Call me at (256) 386-2755 if you have any questions or comments following your review of the report. Cynthia L. Russell Biologist Environmental Engineering Services-West CTR 2L-M Attachment cc (Attachment): Files, R&TA, CTR IB-M SQN July 2005M

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: August 9. 2005

1. Facility / Discharger: Seguovah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 3,266

6. Receiving Stream: Tennessee River (TRM 483.6)

7. lQ10: 2.992.4

8. Outfall Tested: 101

9. Dates Sampled: July 17-22. 2005

10.

Average Flow on Days Sampled (MGD): 1566. 1570. 1569

11.

Pertinent Site Conditions: H-130M molluscicide was injected from July 17-22, 2005. The dates and times for the H-130M injection are in the following table. See Appendix B for complete additional chemical application information during the sample collection period. l Injection Location l Date/Start Time (ET) I Date/Ending Time (ET) Essential Raw Cooling Water (ERCW) Train A 07/18/05 ( 0930 07/20/05 @, 1035

12. Test Dates: July 19-26. 2005

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephales promelas)

Daphnids (Ceriodaphnia dubia)

15. Concentrations Tested (%): Outfall 101: 10.98. 22.0. 43.9, 72.0. 100.0 Intake: 100.0 Pimephalespromelas: UV treated Outfall 101: 10.98. 22.0. 43.9. 72.0, 100.0 UV treated Intake: 100.0

16. Permit Limit Endpoint (%): Outfall 101: IC, 5 >43.9%

17. Test Results: Outfall 101: Pimephalespromelas: IC25 > 100%

Ceriodaphnia dubia: ICQ5 > 100% UV treated Outfall 101: Pimephalespromelas: IC,5 > 100%

18. Facility

Contact:

Stephanie Howard Phone#: (423)843-6713

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone 4: (828) 350-9364

21. TVA

Contact:

Cynthia L. Russell Phone #: (256) 386-2755

22. Notes: Outfall 101 samples collected July 17-22, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were >

100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period. Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Reduction in growth was statistically significant in UV treated intake samples. 2

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101. Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MM/DD/YY)/ Sample Time (EST) Time (EST) Temp. TRC* Time (EST) ID Collected Received (mL) Used By 101 07/17/05 0757 to 07/18/05 1342 2.6, 2.5 <0.10 07/19/05 1313 07/1 8/05 0657 07/20/05 1252 Intake 07/18/05 0636 07/18/05 1342 2.9 <0.10 07/201/05 1252 101 07/20/05 1320 2.3, 2.0' <0.10 07/21/05 1305 07/20/05 0714 07/22/05 1245 Intake 07/19/05 0756 to 07/20/05 1320 1.7 <0.10 07/21/05 1305 07/20/05 0656 07/22/05 1245 07/2105 077 to07/23/05 1305 101 07/22/05 0637 07/22/05 1315 2.3, 27t <0.10 07/24/05 1242 07/2/05 63707/25/05 1229 07/21/05 0722 to 07/23/05 1305 Intake 07/22/05 1315 2.8 <0.10 07/24/05 1242 07/22/05 0622072/519

• TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 1 1.0 0C) in a warm water bath.

Aliguots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart< UV Sterilizer (manufactured by Emperor Aquatics. Inc.) for 2 minutes. 3

Pimephales promelas Ceriodaphnia dubia Test Organisms:

1. Source:

Aquatic BioSystems, Inc. In-house Cultures

2. Age:

22.25-23.75 hours old <24-hours old Test Method Summary:

1. Test Conditions:

Static. Renewal Static, Renewal

2. Test Duration:

7 days Until at least 60% of control females have 3 broods Moderately Hard Synthetic

3. Control / Dilution Water: Moderately Hard Synthetic

4. Number of Replicates:

4

5. Organisms per Replicate: 10 10 I

6. Test Initiation: (Date/Time)

Outfall 101 UV Treated Outfall 101 07/19/05 - 1313 ET 07/19/05 - 1300 ET 07/19/05 - 1200 ET

7. Test Termination: (Date/Time)

Outfall 101 UV Treated Outfall 101 07/26/05 - 1246 ET 07/26/05 - 1233 ET 07/26/05 - 1117 ET

8. Test Temperature: Outfall 101:

Mean = 24.7C (24.1-25.30C) Test Temperature: UV-Treated Outfall 101: Mean = 25.0C (24.5-25.50C) Mean = 24.70C (24.1 -25.30C)

9. Physical / Chemical Measurements:

AlkalinitL, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre-and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics:

Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tideoool Scientific Software. McKinnevville, CA). 4

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 19-26, 2005 using effluent from Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%'Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 98 98 93 93 Test Solutions Mean Dry Weight (mg) (% Effluent) 12(replicate number)_____ (%Effluent)_1 l 2 3 4 l Mean Control 0.696 0.716 0.613 0.698 0.681 10.98% 0.701 0.729 0.679 0.746 0.714 22.0% 0.675 0.698 0.703 0.642 0.680 43.9% 0.678 0.655 0.665 0.625 0.656 72.0% 0.713 0.792 0.726 0.753 0.746 100.0% 0.751 0.653 0.677 0.699 0.695 Intake 0.704 0.660 0.514 0.715 0.648 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TlUa = I00/LC5o: TUc = 100/ IC25 5

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicitv Test. (Genus species) (Type / Duration) Conducted July 19-26. 2005 using effluent from Outfall 101. Percent Surviving Test (time interval use - days) Solutions 1 2 3 4 5 6 7 (% Effluent) I Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100

• 72.0%

100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data (replicate number) 1 l2 3 4 5 6 7 8 9 10 Mean Control 30 27 28 31 28 28 28 30 26 28 28.4 10.98% 32 29 27 29 32 30 33 27 33 32 30.4 22.0% 32 32 35 31 31 28 29 31 30 32 31.1 43.9% 34 35 33 35 32 34 29 35 36 32 33.5 72.0% 35 33 32 37 31 33 33 31 36 30 33.1 100.0% 33 34 34 32 33 31 34 34 37 34 33.6 IC25 Value: > 100% Calculated TU Estimates: <1.0 TUc* Permit Limit: 43.9% Permit Limit: 2.3 TUc 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = I00/LC50: TUc= 100/IC25 6

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2.

Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 19-26. 2005 using water from Intake Percent Surviving Test (time interval used - days) Solutions I 2 3 4 67 (% Effluent) 1 2 3 4 5

6.

7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days) (% Effluent) Data(replicate number) 1 3 4 5 6 7 8 9 10 Mean Control 31 31 30 29 29 29 29 28 31 27 29.4 Intake 30 29 j32.36 28 29 33 30 27 31 30.5 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = I 00/LCso: TUc = 100/ IC25 7

TOXICITY TEST RESULTS. UV-TREATED (see Appendix C for Bench Sheets)

3.

Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted July 19-26. 2005 using effluent from UV Treated Outfall 101. Test Percent Surviving Solutions (time interval used - days) (%Effluent) 1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22.0% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72.0% 100 100 100 98 98 98 98 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 93 Test Solutions Mean Dry Weight (mg) (% Effluent) 1 2 plicate n Mean Control 0.712 0.741 0.761 0.828 0.761 10.98% 0.798 0.798 0.700 0.686 0.746 22.0% 0.700 0.677 0.656 0.780 0.703 43.9% 0.774 0.745 0.748 0.772 0.760 72.0% 0.792 0.788 0.732 0.751 0.766 100.0% 0.720 0.688 0.731 0.921 0.765 Intake 0.596 0.706 0.722 0.654 0.670 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc* 95% Confidence Limits: Upper Limit: NA Lower Limit: NA

• TUa = 100/LC50: TUc = 100/ IC25 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date Time Duration Toxicant Results (IC25) Pimephales promelas July 19-26, 2005 1332 7-days KCI 0.62 g/L Ceriodaphnia dubia July 12-19, 2005 0845 7-days NaCl 1.06 8

PIIVSICALICIIFNMICAI,

SUMMARY

Water Chemistry Mean Values and Ranges for Pirnephalespr-omelas and Ceriodaphnfi dubia Tests, Sequoyah Nuclear Plant Effuent (SQN). Outrall 101, July 19-26, 2005. Test Sample ID Temperature (*C Dissolved Oxygen (mg/L) pit U.) Conductance Alkalinity llardness Total Residual _______Initial Final Initial Final Initial Final (jimhosfem) (mgIL CaCO1 ) (mglL CaCO3) Chlorine (mgIL) Control 24.9 24.4 7.8 7.2 7.84 7.68 300 60 84 24.7 - 25.1 24.2 24.7 7.6-8.1 6.9-7.5 7.68 - 7.92 7.55 - 7.80 290 315 59 61 82 86-10.98% 24.9 24.4 7.6 7.1 7.85 7.66 278 24.7 25.1 24.2 24.6 7.4 7.8 6.7-7.3 7.75 7.93 7.51 7.79 268 296 24.9 24.4 7.6 7.0 7.83 7.63 258 22.0% 24.7 25.2 24.2 - 24.7 7.4 -7.8 6.6 -7.4 7.73 7.90 7.46 - 7.74 236 274 4 49% 25.0 24.4 7.6 7.1 7.79 7.63 229 ioI 24.8 -25.2 24.1 -24.6 7.3 7.9 6.8 7.4 7.68 - 7.86 7.47 7.71 224 -241 7.% 25.0 24.4 7.7 7.1 7.74 7.60 187 24.9 - 25.3 24.3 -24.7 7.4 8.0 6.8 7.5 7.63 - 7.80 7.38 7.71 183 197-100.0% 25.0 24.4 7.6 7.1 7.69 7.59 143 50 58 <0.10 24.9 - 25.3 24.1 24.6 7.3 7.9 6.6 7.3 7.59 -7.77 7.34 -7.70 139 1SO 50 s0 56 62 <0.10 -<0.10 Intake 24.9 24.4 7.6 7.2 7.70 7.61 144 52 63 <0.10 1___________ 24.7 - 25.2 24.3 24.6 7.3 7.9 6.9-7.5 7.60 - 7.80 7.39-7.71 140 151 SI 5 2 56 70 <0.10 - <0.t0 Cnrl 24.8 25.0 7.8 7.6 7.84 7.85 300 60 84 Cnrl 24.5 - 23.2 24.8 - 25.2 7.6 8.1 7.4 7.6 7.68 - 7.92 7.78 - 7.91 290 315 59 61 82 86 10.98% 24.9 24.9 7.6 7.5 7.85 7.85 278 24.6 - 25.2 24.7 25.3 7.4 7.8 7.3-7.8 7.75 7.93 7.77 - 7.91 268 296 20% 24.9 24.9 7.6 7.7 7.83 7.84 258 24.6 -25.1 24.7 -25.3 7.4 7.8 7.4 8.0 7.73 -7.90 7.76 - 7.90 236 274 25.0 24.9 7.6 7.7 7.79 7.82 229 43.9%__ 24.6 - 25.3 24.7 -25.2 7.3 7.9 7.4 8.1 7.68 - 7.86 7.74 7.89 224 241 2.% 25.1 24.9 7.7 7.6 7.74 7.80 187 _____24.7 25.3 24.8 - 25.3 7.4 8.0 7.4 8.l 7.63 -7.80 7.72 - 7.87 183 197 10.% 25.1 24.9 7.6 7.7 7.69 7.77 143 50 58 < 0.10 100.0%_ 24.8 - 25.5 24.7 - 25.3 7.3 7.9 7.5 8.1 7.59 - 7.77 7.67-7.83 139 ISO 50 50 56 62 <0.10 - <0.10 Intake 24.9 25.0 7.6 7.5 7.70 7.75 144 52 63 <0.l0 _______24.7 - 25.2 24.7-25.3 7.3 7.9 7.4 7.6 7.60-7.80 7.64 - 7.8i3 140 - 151 51 52 56 70 <0.10 - <0.10 Overall temperature CC) Pirnephales prornelas Ceriodeiphnia dlubia Average 24.7 25.0 Minimumn 24.1 24.5 Maximum 25.3 25.5 9

PHYSICAL/CHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges for the Pitnephales promelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV-Treated Outfall 101, July 19-26, 2005. Test Sample ID Temperature CC) Dissolved Oxygen (mg/L) p1-1(S.U.) Conductance Initial Final Initial Final Initial Final (limhos/cm) Control 24.9 24.5 7.7 7.3 7.84 7.68 290 24.8 - 25.0 24.2 - 24.7 7.5 - 7.8 6.8 - 7.6 7.75 - 7.91 7.54 - 7.79 276 - 311 10.98% 24.9 24.3 7.6 7.1 7.84 7.65 278 1 24.8 - 25.1 24.1 - 24.6 7.5 7.8 6.7 - 7.6 7.74 - 7.91 7.53 - 7.77 271 290 22.0% 25.0 24.4 7.7 7.1 7.83 7.64 259 24.7 - 25.2 24.2 - 24.6 7.5 7.8 6.6 - 7.6 7.72 - 7.89 7.46 - 7.75 237 - 275 C 25.1 24.4 7.7 7.0 7.80 7.60 236 2.0% 24.8 - 25.2 24.2 - 24.6 7.6 - 7.8 6.4 - 7.6 7.70 - 7.88 7.43 - 7.72 224 - 259 25.1 24.3 7.6 7.2 7.77 7.62 195 z 24.8 - 25.3 24.1 - 24.5 7.4 - 7.8 6.8 7.6 7.66 - 7.84 7.46 - 7.71 185 - 228 i 100.0% 25.2 24.3 7.7 7.1 7.71 7.57 149 24.9 - 25.3 24.1 - 24.7 7.5 - 7.9 6.7 7.6 7.57 - 7.80 7.41 - 7.69 139 - 185 Intake 25.0 24.3 7.7 7.2 7.72 7.60 142 24.7 - 25.2 24.1 - 24.6 7.6 - 7.8 6.9 - 7.5 7.62 - 7.82 7.43 - 7.76 139 - 150 Overall temperature C0C) Pimep hales promelas Average 24.7 Minimum 24.1 Maximum 25.3 10

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected July 17-22, 2005, showed no toxic effects to fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples. Reduction in growth was statistically significant in UV treated intake samples. 11

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimerphales promelas Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephales promelas None

2. Ceriodaphnia dubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephales promelas None

2. Cerioda hnia dubia None 12

PHYSICAL AND CHEMICAL METHODS

1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by EPA Method 170.1.

4. Dissolved oxygen was measured by EPA Method 360. 1.

5. The pH was measured by EPA Method 150.1.

6. Conductance was measured by EPA Method 120.1.

7. Alkalinity was measured by EPA Method 310.1.

8. Total Hardness was measured by EPA Method 130.2.

9. Total residual chlorine was measured by EPA Method 330.5.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein. REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC2s values in g/L KCI or NaCi.

2.

Standard Toxicant: Potassium Chloride (KC1 crystalline) for Pimephales promelas. Sodium Chloride (NaC1 crystalline) for Ceriodaphnia dubia.

3. Dilution Water Used: Moderately hard synthetic water.

4.

Statistics: ToxCalc software Version 5.0 was used for statistical analyses. 13

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002).

3. Methods for Chemical Analysis of Water and Wastes, EPAI600/4-79/020 (March 1983).

4. Ouality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

14

Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling,

Table B-I. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 2

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 I 3

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 . Sodium~tS Hypochioritep mgfL t. .TRC Q. i .-PCL222T I l t Phosphat'. Ž. 4

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 5

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998-July 22, 2005 I I 6

Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets

.DIOMONITORING CHAIN OF CUSTODY RECORD Page_1 of 3_ Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments. NPDES Number: TN0026450 Phone: 828-350-9364 Collected By: Wanda Allen Fax: 828-350-9368 cH Field Identification / Grab/Comp. Collection Datelune Container Flow Rain Event? ColcinDate/Timev~ ImaNo Nc..... sj, tc...;b@ Sample Description Number & MGD Rn Ev F T en Volume (Mark as Appropriate) Dat.i m C ollected Ti mDaea Yes If YMs No Tae-K. a.eTra SQN-101-TOX Comp 7117/05-7/1 1 2 NA57l N Wad le 71/05Express Courier,0/~oo Lz> 07/18/2005of v11 et,,/ Sal C a I Express Courier u""d07/18/2005 ETS 07/18/2005 c-Instnrctions: Clients should rill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6'C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday dclivery or they will not arrive until the following Monday.

BIOMONITORING CHAIN OF CUSTODY RECORD Page 2 of 3 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: NPDES Number: TN0026450 Phone: 828-350-9364 ' Collected By: Wanda Allen Fax: 828-350-9368 Field Identification Grab/Comp C Container Flow Amq Y~"nlPI t 1 t Sample Description Number & (MoD) Rain Ev en Volume (Mark as Appropriate) D Collected .~ Date Tim j, e I e, N re wS~ Am~Trnp' Pyv Tera ppa SQN-101-TOX Comp 7/19/05-7/20/05 2 (2.5gal) NA i. Wanda Allen 07120/2005 Express Courier 07120/2005 b0sa 08Yf g8t Express Aln 07/20t2005 1 xpreTS C_______ 07/20/2005 I , o Exrs Core 072.20 7-7Z > ES0/020 Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via Fedl~x or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

UlOMONITORING CHAIN OF CUSTODY RECORD Page_3 or 3 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One): Project Name: Sequoyah NP Toxicity 351 Depot Street FedEx UPS Bus Client P.O. Number: N/A Ashville, NC Other (specify): Express Courier Facility Sampled: Sequoyah NP 28801 General Comments: C NPDES Number: TN0026450 Phone: 828-350-9364 4 C'44 - 4 Collected By: Wanda Allen Fax: 828-350-9368 Field Identification / Grab/Comp CollectionDateTime Conter Flow ( l F Sample Description ColNumberDat ime(MOD)nerRalin Event? X oa~i e~~'~: Volume (Mark as Appropriate) Collected. DaIf l T Yn

Yes, No Trace

• A8 g

lcTtce nX SQN-101-TOX Comp 7/21/05-7/22/05 2 (2.5gal) NA -Z 1 i SQN-INT-TOX Comp 7/21/05-7/22105 (2.5 gal) NA Sample Custody - Fill In From Top Down Relinquished By (Signature): DateiTime Received By (Signature): Date/Time Wanda Allen 07/22/2005 Ex ss ou 07/22/2005 Express Courier j .26. 07/22/20053 8 ETS 07/22/2005 v Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6C and shipping them in ice. The hold time for each sample is 36 hours from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Environmental Testincr Solutions. Inc. Page I of 6 Environmental Testing Solutions. Inc. Page 1 of6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0) Species: Pimephalespromelas Client: TVA Facility: Sequovah Nuclear Plant NPDES #: TN 0026450 Project #: 1'4i I County: Hamilton Treatment: Non-treated Outfall: 101 Dilution re n information: .Comments: Dilution prep (%.) 10.98 22 43.9 72 100 Effuent volue (niL) 274.5 550 1097.5 1800 2500 Diluent volume (nL) 2225.5 1950 1402.5 700 0 Total volume (nL) 2500 2500 2500 2500 2500 [ Test organism information: Test information: Organism age: zz. ZS -M5 tb J es to L Randomizing template: Date and times organisms l LIZ-OS 1X_3C -i-o %t CO Incubator number. were born between: Organism source: Q1eS BP n-cIt-OS Artemia lot number. v Transfer bowl information: pH Temperature = C Total drying time: 2-l.l o0J i& .zIA-3 DatelTimein: rZ4b 131O Average transfer volume: Date/ Time out: 01.1

1. 1 %

Oven temperature: Daily feeding and renewal information: Day Date Morning Afternoon Test Initiation, Control water Sample numbers Analyst feeding feeding time renewal, or batch used used I _time termination time t__ 0 cri-o S C1 ( 3 Ls -O

t.

t0 I 02-d alc-'ZO-Oi rzS AO %mm 2-. (31iiIOS6 0 I R. 01 XO2-z 2 al zt-e S Mao So3c5 t 13-6 cscfl2o.O5 & A .3 Q1-22.-O DROS _S_ 2I4 avi34B-% oi sao.ok4 OL 4 CT1-24c0s 09m 15c1A 1-3a5 0M-1-65 Mr C0a 22.O I 0l-I 5 11 2'k-2 05 M:5-l56 I I ZA 7 al"-i2 gYi Ak S01i2.08 Soz 6 ali-25-05 otsz tASS 1 z 2 al .XS A, 6s n a.6V4()a 2_ 6 £32 O65 +/-'~;W^ 1 2-z'tl 2i-+/-. _Q .¶>;- -A j Control in formation: Acceptance criteria Summary of test endpoints: % Mortality Al S 200/ 7-day LC50 l I Average weight per initial larvae: O . 0 NOEC 1007., Average weight per surviving larvae:

0. LAI 0.25 mgfarvae LOEC l

OC)', ChV IC2s 1 >1 0073

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Pimephalespromelas Date: 1i 4-0o Client: TVA / Sequoyah Nuclear Plant - Non-treated Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I J K L 0 10 IC) 10 It tO 10t 1 tO 1 0 I 10

10. 100 10 (I)D 10

/0 IC)/0/ 101ID/0 /00 o/ 2 IC )t) /0 -to O0 )0 JO JO to to lo tO lO lO (0 tO lO to 10 ID 10 to /0 4 / 11) 16 10 J )) MDZ)} } e /0 1L l 10ID 1b 1 M 10 J 10 10D l 10 6 l 1010l)10 /010 10 1(

10) to 1I)

?1) I o 10 IC to 10 10 I O* 1U A - Pan weight (mg) & a Colorlde t~f ratin 16.2 I6 1.- I S-IL M4 S 1q.8S6 1.,5, 110.2% I 16. 4.,1 2_ l.

• 9 16.8 lL,31 B - Pan + Larvae weight (mg)

Analyst: KT__ _a a3. a 3.4 at a1. 1.93 I.)13 3 23.0O _ 3.l1 2 .ai 3 Larvae weight (mg) - A - B w lb.Xs (A& 1.o\\ 1n I.z.1i 5 .L1 ti.1S _

7. o3 Z

Weight per Initial number of larvae (mg) - C I Initial number of larvae 40 0s 'IO-V7tr A O. N 0'4 O- ,'14 to 'a.\\ o0 _~ a

• a
• a
• a
• a Average Percent weight per reduction InitIal from control number of

(%) larvae (mg) a 1( 0.-%'A LL Sol. 0.2 T0 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig - unusually large, d&r - decanted and returned, w = wounded. a Calculations and data reviewed: Comments:

• -=.

.k.OU .;: s .,e Page 3 of 6 Environmental Testing Solutions, Inc. Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Date: a-Iig-oC; Survival and Growth Data Day 43.9% 72% 100% M_ _ N O P Q R S T U V I W X 0-1 iooi _ 0, 10 1t 10l0(b iO IO 1 10 / 0 10 1 0 10 / 0 1 0 10 10 / 0 10 1D 10 c 1 O 10 10 tO IC 10 lO /0 l I0D II 1 _1 0 I 0 1) I 10 10 10 10-0IQ10 10 1QI1Dli_/O 10 _0 0 D I0 10 O I iD I 10 10 6 JQ 10 10 10 I010 ID I lb 10 10 l0 m a0 I ' o to to 10 to /O to 76 I J CC) 10 1( I ( slC) O 0 ,l O _-l A Pan weight (mg) _ _ _ Color Idetlcto: X 1 jt.,r 1e1.-, J6.21 1. ol 1.5.11 1 Ziz Iq.j I Iq.SO 1v*s IS. 10 1q.9" I.31 Analyst: B -Pan + Larvae weight Anaglyst: aR .la3 a ma,+ U; A10 2%1*1f43A3 ls \\6a Larvae welght (mg) - A-B b,8 55t.us (t.,S 1's31.92 A7.2 ,5 s n z Weight per Initial number of larvae (Mg) .4 ClInitlainumberoflarvae ° ' O * °' I t O OO0 Average Percent weight per reduction 0 .60_q Initial from control number of (% la r v ae ( m Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. e-V, Page 4 of 6-'-5 I.. Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: n-l-Ct)S Survival and Growth Data Day 100% Intake Y Z I AA BB 10 10 IO I/ 2 1t 1 10 /0 3 ((0 10 10 4 to .5 ID 10 6 ID 9 10 7 .lo 1 A-Pan weight (mg) L. Color Identlfl tlon:-A, .2Ai I.Az IS.23 j&..39 Analyst: r Z B P Paa + Larvae weight (mg) Analyst: Larvae weight (mg) - A - B Weight per Initial number oflarvae(mg) '.0 C1 Initial number of larvae At O ) Average Percent -e weight per reduction Initial from control numbero-(%) larvae (mg) O. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m - missing, sk = sick, sm unusually small, Ig - unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments: S -re t 7; e~t GCr6PMMS Ue v6our~b Fy

TVA/ Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26,2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephatespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Clieat Test dates: Project number TVA/ Sequoya Nuclear Plant, Outfall 101 -Nan-treated July 19.26, 2003 ,991 Revehwed JM~ in C-' i--r A,....t WIAIn. I Ch~an~('X Ceaw Ia (O) 8 i t Cat..

• .f h

A-_IekS ( _b 8-u"a L. ( u W.U*h 9..b04-MW-u.ig*I*-4 -twit t Cu.Irk .t* t*.e.4 45 he( -A. -A rtla 0.. a1.eaf ... c) N he.'a (._ ( afthu... P.uP.WP ..n.I (% A 1 0 10 16.24 23.20 6.96 0.696 0.696 Control II 10 to 16.24 23.40 7.16 0.716. 0.6t1 6.t 0.716 100.0 0.66t 6.8 Not applicable C to to 15.14 21,27 6.13 0.613 0.613 D 10 10 14.95 21.93 6.9S 0.693 0 698 B 10 to 14.86 21.87 7.01 0.701 0.701 to."% tO 10 16.32 23.61 7.29 0.729 0.714 4.2 0.729 100.0 0.714 4.2 .4.8 G to 10 16.21 23.00 6.79 0.679067 U H 10 30 36.30 23.76 7.46 0.746 0.746 1 10 30 14.72 23.47 6.75 0.675 22% 3 10 30 16.19 23.17 6.98 0.698 0.680 4.1 0.691 100.0 0.680 4.1 0.2 IC 30 tO t6.28 23.31 7.03 0.703 0.703 L 10 10 16.31 2273 6.42 0.642 0.642 M to 10 16.33 23.11 6.78 0.678 0.671 U39% N 10 s0 14.75 21.30 6.55 0.655 0656 3.4 0.655 M.0 0656 3.4 3.7 O I0 to 16.23 2288 6.65 0.665 0.665 P 10 30 15.09 21.34 6.25 0.625 0.625 Q t0 30 15.1l 22.24 7.13 0.733 12% R 0o to 16.28 24.20 7.92 0.792 0.146 4.7 0.792 tW.0 0.746 4.7 .9.6 N 10 10 14.9132217 7.26 0.726 0.726 T 1 6 0 0.53752.1 T lo

418 73 sz U

to lU 13.10 7.51 0.7 1, 100% V I 110It t S.0 1 21.63 I 6.53 f 0.653 W l 10 t0 1 14.99j 21.76 1 6.77 I 0.677 0.695 6.0 0.653 0677 0.699 800.0 ILO Act 30 l0 16.37 23.36 6.99 0.699 1-I" 9 0.104 100%1WAk 0.699 5.6 0.660 0.514 0.715 925 0.648 14.3 4.8 o trall let: Dusett's MSD value: .MSD: Intake: Dausette' MSD value: PMSD: 0.0592 8.7 0.1005 14.8 MSD - Minkim Sipifiunt Differance PMSD - Percent Mmknsn Sigpifnt Diorenceo PMD50 i a nwmea of tat preuckl The MDtstb the ninihn Im percent diffemnc betwen the cntrol end trusbnot that ca be declared statiticolly viificnot hi a -hole emnunt toxity teat. Ona g

• aipi t diffrence occm cfc EntvironerntateteingSolutmo, leo. crohi tokichytlsh when a toxiet rduces Ptpholes goh by 13.4% fiomthentrol (detrenned thru retarence tozicant leaSins).

LowerlMSboanddeternmedbyUSEPA(IOth peretie) -9.41 UpperPMSD bond detined by USPA (90th pt til) -35K Th lowetnodupperbound werecalcuatd bythe USEPAwsg205 tueaodutted dromnt 9 bbortories forlmephales 1 hI chrnicrefernce toxiant teats. USBPA 2000. thdiWdinud ounting for Method Vaiabty iWhole Effluat Toxity Applia Under theNaina Polutut Dscha Elirneioo Nipm EPA-133-R-00003. US Eavio taPtotedion Agec Cimncnati 01

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Statistical Analyses Luvt r~I Cis., sad SorviTeat.7 DsyCr..r SunDate 7119r/205

TtID, PpFRCR SmplelD:

TVAISuhNucierw nt.O R 101.N n-tated d Dte: 7/262005 Lab ID, D Eavr. B aTaft Sat Smple IYPC D&.DdipsRs]Wp pot Sakl Date. Pwtocot PW FWCtR-PA21.R42-013 Teds Specie: PP-hopbaes pneleu C"a_% 1 2 3 4 D-Concol 0.6960 07160 06130 0.69t0 10.93 0.7010 0(7290 0.6790 07460 22 0.6750 0.6910 0.7030 0.6420 43.9 0.6no 0.6550 0.6650 0.6250 72 0.7130 0.7920 0.7260 0.7530 100 0.7510 0.6530 0.6770 0.6990 Csse.% Mess N-Mes Mess Min Mal CV% Y N I-St Oi"l MSD Mea N-Mesa D-Col" 0.610 1.0 0.6101 06130 0.7160 6.765 4 0a6973 I.OXO 10.91 0.7138 1.04t5 0.7138 0.6790 0.7460 4.15

• 4
• .1343 2.410 0.0592 0.6973 1.00 22 0.6795 0.992 0.6795 0.6420 0.7030 4.093 4

0.051 2.410 0.0592 0.6941 0.9954 43.9 0.6558 0.9633 0.6558 06250 0.6710 3.440 4 1.017 2.410 C00592 0.6941 09954 72 0.7460 L.09S9 0.7460 0.7130 0.7920 4.671 4 .2.655 2410 0.0592 0.6941 0.9954 100 0.6950 1.0209 0.6950 0.6530 0.7510 6.013 4 .0.380 2410 00592 0.6941 09954 AusvIlsryTeT Snttioe Cttles Skew Ktrt Sbapwro-W s Tat 3ndmata noemal dnI-utvn (p > 0.01) 097tJ6490 O914 .027199037 44161453 BNdWks Test ktdxats equal viaca (p-O.t7) 1.32396853 15.01627224 Hyll lshw6Tel(.h 0.0S) N4OEC LOIC EkV TU MSDs MSDp MSB MSI -PFrsb if D=netfiTest 100 >100 1 0.05923351 O.07022236 0.003949075 0.001211itl 0.0213543

• 5f1 Tr t

"vs D-Cmifrol Llr.arlatrp.letd (20 Reles) point SD 95% a411p) Skm IC35 >100 ICIO >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 IC50 >100 0.7 0.8 0.2 0.1 0.0 s -0.2 0 20 40 0o sO 100 120 Do % D0°J39 p

s.

14*8. 0.05 Iel 0.6 i^r= 0.5 0.4

0.

3 0.2 C.1 8d spJ07-19-05datr

TVA I Sequoyah Nuclear Plant, Intake Non-treated July 19-26, 2005 Statistical Analyses Lu-d Fimb CGwn and Sr+awl Test-7 Dsy Crrwtk StatDec 719r=0T5 TntlD: PpFRCR Sampl D1. TVAl SqhNuckarPlat.IntakIe -N4=4-d En Dl) 7/26/ S 1 lAf. ETSavwk.Tatig s50 Sampla 7e: DMR-Dschage M.onitrn Repoit Sample Dute: rtoott WFWCRHEPA-12l.R0213 Tart Species ?P-PThbaks pr hsnek Cew-V. 1 2 3 4 DCol 06960 0.7160 0.6130 0.6980 100 0.7040 0.6600 0L.140 0.7150 Tr femnnwUnramtsruod I.Tclbd isatic CONc.%. Mean N.MM. Ma" Mil Mat CV% N Meant a MsD Ma. N.Mmm D-Contol 0.6t08 1.000 0.6t0t 0.6130 0.7160 6.765 4 0.6S08 1.ODO 200 064n3 0.9523 0.6J43 0.5140 V7IS0 14.5 4 0.629 1.943 0.1005 0.6423 0.9523 AzziliaryTests Sbtkdc rIcal Skew Ken Shapio-WilbTatidica notmaldar buton (p)0.02) 0.114729613 0.749 .1353771 1.253381696 P-Tat bdkato equal valkwn (p - 0.28) 4.043024063 47.46722794 Hypothes Tat? (IcbM 6.) MSDv MSDp MSB MSK 1.2re 6f Ha.omoatr0Ta ztdtamd tasnou igamn difftro 0.100412498 0.147605579 0.0021125 0.00534797 0552t33319 1 6 Tratmt we-ontrol LU.e.r ls.trpolath (200 Rmampta) P.t SD 95%VCAErp) Skew ICQ5 >10 tC10 >10W ICis >100 1.0 1C20 >00 0.9 IC25 >100 IC40 >2W0 0.8 tC50 >100 0.7

• 0.4 03 02 0.1 0.0.

0 20 40 s6 80 1M0 120 Do" 0.0 0.7 0.6 14a20.Os 0 .2 o( ~gtg~fcarnc. 0.5 0.4 0.2 0.1. O0ws s07.1 9OSdoaa

I Envia T g S Environmental Testing Solutions, Inc. Page I of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnia dubia Client: TVA Facility: Sequovah NuclearPlant NPDES #: TN 0026450 Project #: I A&i I County: Hamilton Treatment: Non-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) 10.98 22 43.9 72 100 Efuent volume (mL) 274.5 550 1097.5 1800 2500 Diluent volume (mL) 2225.5 1950 1402.5 '700 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information: l Organism age: , zA-90-3S.& OQt: Randomizing template: q.LnuU3b Date and times organisms riI-c15 oMOS -M 1OL4. Incubatornumber and I were bom between: shelf location: ZZ43 Organismsource: ai-VZ-oS A-k YCTbatch: 042545 Transfer bowl information: pH 7- .8. Temperature - Zs.2 Selenastrum batch: Ut3* al-Ib-OS Daily renewal information: Day Date Test Initiation, Control water Sample numbers Analyst renewal, or batch used used termination time MAS 0 a1 -A-S Air cr-16 05 6 0sra1ltt. c 94O2 0 2 r 7n-0* jQD 3l-tbS6 OscrlR.o0 CO2Z. c2 -,zzos J L OI I I1 a no 1 02 .0 iaC 4 0; a41-23-S llO2 C05 ts0os o 1z2.o 7 0d2. s cri-A i-OS 137Z ._____A __.__ I 6 aiizS-o 112.l 1 007. % Adults having 3Y Broods: 10% k 80X NOEC IO% % Mortality:- 07 7. s 20/. LOEC I It'7. Mean Offspring/Female: 2aA 29.'4 2 15.0 offspring/female ChV > 1007. %CV: S7.37 T17 c40.0 % IC25 > I1C017

Environmental Testing Solutions. Inc. Page 2 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: 0l-7-A-I CONTROL - 1 Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 l0 I You ngiprodu1ed O +/- r Adult mortality 2 Young Producedr o I 0 5 O C O Adult morhailty L-I L-L- L-L-1> _1 = 3 Young produced 0 C Q O Cl O l0 0 V : T Adult mortality l I L 1 7i 7 4 Young produced LA .22 37IT I Adult mortality l9 L._ L-L- L-l l lC s Young produced 9 jq l l otq Adu lt Cm rt. Ci L- `1. C. I-t-I 6 Young produced 0 C I's I) o TIs Adult mortality C ,-t t 7 Young produced Ito 11 0 I11s I a 0 0 Total youngprodueed l ll l8 I l ZS 7-lp0 l 2 2 Final Adult Mortality IL l C L l X f Broods X. I .1 I Note: Adult mortality (L - live. D - dad) C Concentration: I l %Mortality: l eY I Mean Offspring/Female: g 1 CONC: 10.98% Survival and Reproduction Data Replicate number I Day 1 2 3 f4 5 7 8 9 1 lI lYoung produced l =s l()4O ) lO lC Adult mortality L-L - l 2

lYoung produced l 1

l l) 0 l Adult mortality l C_ L (_ l l l l L l l. 3 Young produced C)0 4 Young produced 1 i ILA L S llAdult mortality l U.-C 1_, L.. Adult mortality L-'-~ L-S Young produced C I lF C l O l 6 Young produced 0 c) 0o I 0 0 L ItGl Adult mortality L-C l IL-l. C-1-. C.. C.l Total young produced l 3l9 3Z l 3° 33 l Zl3 l 3 Final Adult Mortality C C l NOte: Adult mortality (L -live. D - dead) I Concentration: IMortality: : a I Mean OffpringfFemale: I --I. q l I % Reduction from Control: I -1. 070 1

Environmental Testing Solutions. Inc. ";7,.M .:. -,. v.
. .11

;..... I Page 3 of 7 I

Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date:

t.

q-cS CONC: 22% Survival and Reproduction Data Replicate number D 1 2 3 4 5 6 7 8 9 10 I Young produced 0 l O ) t A ( ) C O Adult mortallt _L-L L 2 Young produced 101 l O n o O o Adult mortality T 3 Young produced O DCO) 1 O Q. 0 c o Adult mortality L L +-L L.- L-- L 4 Young producedl LA Lt* 774q S q e Adult mortality 1 1 3 Young produced I C

1.

I Q 0 ... C Adult mortality (L-L 6 Young produced o O O CO IS C> -i l Adult mortality L_ L,. L-. L..- 7 Young produced L LP 2 1 Iq Jj j1-1 OI L JLC L Total6 oung produced 3z Cz 0S 3l

• 3t 1

ll Ttlyug rouej 3 Z 3 S

5 i S

7-9t 3 1 .30 .32. Flnal Adult Mortallty, 1 U l 1 l L t Note: Adult mortality (L live, D -dead) - Concentration: % Mortality: 0 I Mean Offspring/Female: I 3\\, % Reduction from Control: - .ST7e CONC: 43.9%. Survival and Reproduction Data ~~Replicate number DaY 17~ 2 3 4 1 6 7 a 9 10I l Young produced l ) 0 C l p O ( Adult mortality U C1 U L I ~ .U 2 Young produced fr l C) 0 l l Adult mortality U C I U L T 7 L 3 Young produced lU) 0 lQ

• l C)

£). 0 - Adult mortality ~ U T U .U Young produced l S l tL l SI 4 L1 l 5 Young produced I

fZ l\\1l1 O

I Is I n l 19 lZ L S YoAdult mortalio c L-I-L. L l-ll AL 2_3j 12 _Z I FAdult mortality = L L 7 1 Young produced [ 1= 9 16 a- -s ( Total young produced l 31 l 35 l Ih3 l Flnal Adult Morblity I l-l § U. l VUM. ^Aulttmortality (L-live.D-dcad) oj ex e.A Concentration: I Mortality: 1 0% 7 Mean Offspring/Female: l 33. l I % Reduction from Control: I - I g.O Z

. i FEnvirnrmental Testing Solutions. Inc. Page 4 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant-Non-treated Date: oai-lS-S CONC: 72% Survival and Reproduction Data Replicate number Day 1 2 3 4 S 6 7 8 l 9 l 10 I Young produced r) O j O _ O _ Adult mortality . L _ L 1 Young produced O 2C) O C _ O C) Adult mortality L L 7 Young produced - O __.O_ Adult mortality L ( L-U t L Adlt rtlty I L V -I L_ L- ŽL-L- 1 L 4 Young produced 2* I - 3 Adut mortality l t 6 Young produced 0 z 13 19 1 Adult mortality L 1.- L-I L-L. 6 Young produced 0 0.;. ZO I)1 0 O Totalyoung produced a .3Z 31 31 1 33 33 l 3L l30 FinalAdultMortality 2 t 2 T l Note: Adult moality j L live D - ead < 4lConcentration: % Mortalityr 07 Mean Offspring/emale: 13. % Reduction from Control: IL.S7 0 CONC.: I 00%07 r W

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. Survival and Reproduction Data

• Repliatneumber Day

'-^ l - 3/4U' 2 i 3 4 l 5 6 7 l S 9 l 10 l lYoung produ~dl........ O lO lO l lC) l O O. O C) -lAdultmr r;-e i 6 wo 1 Young produced -l l0S Adult mortality l i L. L 1 3 Young produced 'O1 Adult mortality - U' l T J l ll 4 Youn~gproduced 1-L °l() i Li I's ( ) ~ Ts Adult-ort -ty l L. L L L L Young produced. 0 0 3 1 3 I Adult mortality L l N CLl llIIl L 6 Young produced o DN ( -27) Adult mortality U j LU ~ 7 Young prod uced jt 1'1 Ttlyug produced 0 0 3.m2 37 z A du t oraItyLAd ult U. U Note: Adult mortality (L - live, D - dead) l Concentration: %Mortality: (V. I Mean OffspringWFemale: 1 23. £, % Reduction from Control: -ta.37,

I.. .^, I I Page 5 of 7 Environmental Testing Solutions, Inc. Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Date: O1-l0-Os CONTROL - 2 Survival and Reproduction Data Replicate number Day 1 2 3 4 l 5 6 7 l 8 9 10 I Young produced Q j) Qi QO Adult mortality \\ L L* 2 Young produced O ) O O O _ Adult mortality L E C L 3 Young produced OO7-OC Adult mortality7 _ _ L 4 Young produce d 5Ll -5 Li 'A 5 5 5 A-ult mortality L-L - S Young produced l 0{O9l Adult mortality L 6 Young produced O O O l Adult mortality L. L-L L L-L L 7 Young produced [I I's 0 IL0tC) 5 O C .1(.. Total yougng produced 3 Zq Z Z 3 Final Adult Mor orlit a L; L Note: Adult -ortality (L live, D dcad) Concentration: lMean Offipring/Fernale: Z9.;1 CONC: 100% Intake .and Reproducti'on Data I Young produced l l r9 0 0 r Final wdult mortalAty M L Li. L i-L Li.. L Li1 l2 lYoung produced lO lOl< jl( Nate: Adultdmortalty lI'L lie t dI Mea Of

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3 5 l Young tproduced l Al l vCnl 1R eprodu to Data Adut m rtlit 1L . 2 =.3 4 5 6 7-S I l Young produced l

• 4 Q

4 QO Dl. l* Adult mortality L L-' .' L. L e t L -I.. %u-L- ~ s Young produced to,.L~ ) 4)1 lo.. C-Adult mortality Li L-LL LiL-L- l Young produced l C - Adult mortality C T T 7l IJL lll 7 Young produced I IzS L.

i

- -w

• r o n ro u e L

J ) o 6o a Y oun g prouc d uced ( C ) 0 9 05 Final Adult Mortality t i U. L i I tW Note: Adult mortality (L - live, D - dead) I Concentration: % Mortality: O -Mean Offspring/Female: l 30.5 I l- %Reduction from Control: l-.7

TVA I Sequoyalh Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Verification of Cerladaphnia Reproduction Totals Control-I Day ieptiW 5 7-j--ber ~ j Total 1 0 0 0 0 0 0 0 0-0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 -5 9 9 9 30 9 10 10 11 9 9 95 6 1I 0 0 0 15 0 0 IS 13 16 60 7 16 14 16 17 0 15 13 0 0 0 91 Total 30 271 231 311 28 23 23 30 26 23 284 10.98% Day RepIi vate nunherToa Dy 1 2 3 4 5 6 1_ 7 9 0 Toa 1I_ 0 -0 0 0 0 0 0 0 0 0 0 2j__ 0 0 0 0 0 0 0 0 0 0 0 -3 0 0 0 0 0 0 0 0 0 0 0 4 5 4 4 4 5 4 4 4 S 6 45 S 110 10 10 10 I11 10 9 10 10 101-6 0 0 0 0 17 0 0 14 18 16 65 7 16 15 13 15 0 15 19 0 0 0 93 Ttl 3 2927 29 3230 33 27 33 32 304 22% Da2 Replicate inumber 3- -9 1-Total 273 43s 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0-0 0 -0 3__ 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 3 4 4 4 40 S 121 11 It 10 12 10 10 10 11 11 103 6 0 0 0 0 15 0 0 17 15 17 64 7 16 17 19 107 0 14 36 0 0 0 9 Total 32 321 351 31 _31 1283 291 31 303 11 43.9% Day Rep2licate nlunter Total 5 6 7 31 - 9 1 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 10 0 0 0 0 0 0 4 5 4 S 614 4 4 4 S 4 45 5 1012 12 10 I1113 30 12 12 10 1112 o 0 0 12 19 18 1 92 7 19 19 16 17 0 0 5 00 0 3 Total 341 35 33 351 32 34 2 3536 132 1335 72% Day Re lIcate nuWmttr Total I 2 ! 3 4 5 6 7 89 10 1 0 0 0 0 0 0 0 0 0 0 0 3 00 0 0 0 0 0 0 0 0 0 4 5 6 4 4 4 5 4 4 4 4 44 5 1 1 1 1 12 13 12 12 11 10 13 10 115 6 0 0 0 0 0 0 8 13 7 1 9 1 6 70 7 _191611620 1516 000 0 .302 Total 135 331 32 37 31 33 3 31 6 30 331 100% Day Replicate nuiter Total 1 2 3 4 5 6 17 3 9 10 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 5 4 4 4 6 S5 4 45 I 2 1 10 I 11 1 13 10 12 1 1 114 6 0 0 0 0 0 0 0 0 20 19 39 7 17 -F-171917 13116 15 19 0j 0 133 Total 133 34-34 32 331 31 3 4 3L4 37 34 336 Control-2 DyRep Icate nunter Total 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0-0 0 0 0 0 3j 0 0 0 0 0 0 0 0 0 0 0 -t1 3 9 9 30 10 I30 9 9 95 6 0 0 0 0 1 0 14 13 17 14 74 7 116 171 015 0 0 0 7 Toal 31 31 30 29 29 2 29i 2 31 27 29 100% Intake Day 1 2Replunte nunber - Total I I~ 3 4561789 10 0 0 0 0 0 3 0 0 00 0 0 0 0 0 4 4 4 4 4 44 4 4 3 4 39 5 11 10 10 13 9 10 13 30 10 I1 3 07 6 I0 10 0 5 0 3 16 0 3 6 48 7 15 15I 1o3 lgI 19 01 15I 0 14 0 111I Total 30T 291 321 36 123 291 331 30 27 3 0

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quallty Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA/Sequoyah Nuclear Plant, Outrall 101 -Non-trcated Test dates: July 19-26, 2005 , Projed number 1991 Revelwed by: -- Concentration Rcate number Survival Average reproduction Co n lket of Percent reducion from (M) 1 2 3 4 5 6 7 8 9 10 (%). (offsprlngl'iemale) varation (%) pootedeantroti (-/) Control - 1 30 27 28 31 28 28 28 30 26 28 100 28.4 5.3 Not applicable 10.98% 32 29 27 29 32 30 33 27 33 32 100 30.4 7.6 -7.0 22% 32 32 35 31 31 28 29 31 30 32 100 31.1 6.1 -9.5 43.9% 34 35 33 35 32 34 29 35 36 32 100 33.5 6.2 -18.0 72% 35 33 32 37 31 33 33 31 36 30 100 33.1 6.9 -16.5 100% 33 34 34 32 33 31 34 34 37 34 100 33.6 4.7 -1S.3 Control - 2 31 31 30 29 2 29 29 29 28 31 27 100 29.4 4.6 Not appicable 100% Intake 30 30 27 31 100 30.5 8.6 -3.7 Outrall 101: Dunnett's MSD value: PM*SD: intake: Dunnett's MSD value: PMSD: 2.014 7.1 1.624 5.5 MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces Ceriodaphnia reproduction by 9.2% from the control. Lower PMSD bound determined by USEPA (10 d percentile) - 11%. Upper PMSD bound determined by USEPA (90' percentile) - 37%. Tle lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic reference toxicant tests. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Efuent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-S33-R-00-003. US Environmental Protection Agency, Cincinnati. 01 l.

IVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated July 19-26, 2005 Statistical Analyses C.oci~ehals SurvtuJ and Rtprodac.6 Tut4teprodu.cti Stt Dat: 7119/090 Tat 1ID: CdFRML Sample ID: VA/ Sequoyh Nulear Plbnt, Outll 101. Na.etre EcdfDate: 7rs LabID: E a EniTestitgSol Sample Type: DM R.DMchwVcf kgctapm Sample Dat: Potocot FWCHR-EPA4121-R4.013 Tat Species: CD.Cvdaph" dubk Camentr CaC.Y. I 2 3 4 S 7 a 1 D.Cmtml 30.000 n.ooo 21.o0o 31.000 23.00 21000 2.000 30.00 26.000 21.000 10.9 32.000 2 n0eo 27(0O 29.000 32000 30.000 31.000 27.00 33.000 32000 22 32000 3.000 35.000 31.000 31.000 21LO0O 260O0 31L.OO 30.000 32.000

43.

34.000 35.000 33.000 35.000 32.000 3400O 29.000 35.000 36.000 321000 72 35.OW 33.000 32.0D0 37.000 31.0L 33.000 33.000 31.000 36.000 30.000 100 33.000 34.000 34.OO 3 32.000 D30 3100O0 34.000 34000 37.000 34000 Trulofra:1 v Umateaf4.d I-Tsiled lsetik Cme-.% MIem N-Mess Me" Mb Mar CV% 0-Stat <idnl MSD MfeDa NMesD D-Cznml 23.400 1.0000 21.400 26.000 31.0 5.301 10 31 6L3 l.000 30.93 30.400 1.0704 30.400 27.000 33.000 7.621 10 L270 2.27 2.014 31.603 l.ooo 22 31.100 1.0951 31.100 21.000 35.000 6.141 10

• 3.065 2.217 2014 311433 0.0000 43.9 33.500 1.1796 33.500 29.000 36.000 6.174 10

.5.790 2.217 2014 31.613 0.00o 72 33.100 1.1655 33.100 30000 37.00 6.197 30 .5336 1227 2.014 31.613 l.o0 100 33.600 1.181 33.600 31.000 ".oo 4.69S 10

• 5.903 2.217 2014 31.613 1.0oc Asrtubx Ststk Olcal Skw Klrt 1Ctarju~YD Test stats noOCma08n6,tchf (p > CL01) 0.509913653 1.035

-Q053 1424 -0.27574359 Bartlet, Test indicates eqmlvcea (p -073) 2.774007797 15.01627224 lvlythes Twt(lXbil, 6.06) NOEC LOIC ONv TU MSD. MSDp MSB MIK F.4rb it Doemetr Test 100 >100 1 2.0142420S 0.70924233 43.4966667 3.t7s62sa 1.90-07 5.54 '1eTtnnt Ds0Cefol Llaer 3.kQltatiu (20 Rassespt) PF.1t ED 99% CL Skm ICos >100 ICIO >100 ICIS >100 1.0 3C20 >100 0 s IC2s >100 o s 1c40 >300 0.7 3CS0 >100 0.6 0.4 o3. 00 0.2 0.1 0.0-40.1'. .0.2 0 20 40 so 80 100 120 Dor; % 4004 T-TI..M Dess4&9ip0us ll"I l25 0o19fa~i~ca ,f alglfir. I B o0 ^ 2& gO7-J9-05ddafa

TVA / Sequoyah Nuclear Plant, Intake Non-treated July 19-26, 2005 Statistical Analyses Caidaphis SsrvfvJ sad Repredertla TatRteprododcts S~t Dte: 7/19/2005 Tnt ID: C dFRC Sampl) ID. VAI Saquoyah Nnka rPkn Inlah-NM-trWd r Date: 26/2005 Lab D TSEnv. Tetki Sol Sampe TYpC DMR-Dbc uFaro Mwoh cpmt SompleDate: Protocot FWC8R-EPA-U21R4024013 TestSpecks: CD-Curdopbnsdubie C~RsC% 1 2 3 4 5 7 3 to D.Ctol 31.000 31.000 30.000 29.00 2.000 29.000 29.000 23.000 31.000 27.000 100 30.000 29.000 32000 36.000 21.000 29.000 33.000 3000 27.000 31.000 Theess tlfruuier~ I-T iled Iils~ Caae.% Ue. t4Mes Mess Mb. Mel Cv% IV 0-361 t 1 7tSD Mess NMes D-Control 29.400 1.0000 29.400 27.000 31.000 4.591 10 29.950 1.OOO 100 30.500 1.0374 30.500 27.000 36.000 .640 10 -1.175 1.734 1.624 29.950 1.0000 AuxI~oryTeefs Masis.e QMIC11 Skew Kurt SbaprO.W&kl Tet Wwatmw -1 dskl.tm (p > 0.01) 09440490.5 0a 6 0.799423706 1.55296927 P-Test bdicates tqutnrine (p - 0C06) 3.110975552 6.541089535 Ilypestes Test .os MSDv MSDr MSB M 4lt F-Preb di omioscedutic tTestkdicato nosipifrnldufdmee 1.A23612015 0.035224901 6.05 4t3333353 0235370061

1. 1 T.uunntmais D.Cocfrol Lisest Interpolato (DO0 Itasels)

PIsot SD 5% C Skt IC0S >100 ICIO >100 ICIS >100 1.0 IC20 >100 01 IC25 >100 IC40 >100 M50 >100 0.7 ~0.3 02 0.1 t.O

• 0.0

-0.1 0 20 40 0o o 100 120 Do. % Des.-PespemsePWCs 40 35 30._ F 4.8 Af 0.06 k I 25 ~1 S 10 5 1t sf-1r; lcat. 1.4 w_07-49-OSdta

Environmental Testing Solutions, Inc.

I, :

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% m-Au -. a nnn n% kAllUII1L, TVIIUIB £LLIUtLILL AUALIL3 1a U I L A o I J A1ZVnUu I UUV.U) Species: Pimephalespromelas I I I I I I I. I I' I 3 Client: TVA Facility: Seguovah Nuclear Plant NPDES #: TN 0026450 Project#: 1 qcl County: Hamilton Treatment: UV-treated Outfall: 101 Dilution preparation information: Comments: Dilution prep (%) _ 10.98 22 439 72 100o Each concentration was treated E~uent volumc (rnL) 274.5 550 1097.5 1S00 2500 for 2 minutes with a UV sterilizer Diluentvolurnme(mL) 2225.5 1950 1402.5 700 0 to remove pathogenic Total volume (mL) 2500 2500 2500 2500 2500 interferences. Test organism information: Test information: Organism age: 1z

• b.S 1oJS.

OLb Randomizing template: tUe Date and times organisms tn it a0b os 'O M AScO Incubator number. were born between: Organism source: ASS. GAvv cSI-te-QS Ariemla lot number 1rlQ Transfer bowl information: pH Temperature = C Total drying time: 1.S9. Zql-Date / Time in:% z6-C3 i3 l O Average transfer volume: Date/ Time out: oc-zi. os 83 Oven temperature: l* C.' Daily feeding and renewal information: Day Date Morning Afternoon Test Initiation, MHS Sample numbers Analyst feeding feeding time renewal, or batch used used time termination time Cn-ls-oS S36 -r S Osilk.od I 01-2OOS oCc °O-lO'S 1 05 Cn.oL 'Cot 2 ___z__S ClS3 tSO6 17A1 I O,.

• 3So B ouo1 z.

o3W O4 .3 ai-zz-o; QOKr5 tSoo 17131 0113 'SE,2 OS~f7o-zo_3'i`O Jr, 1 0z3-OS oneo %5it4 A 21c orti3.Cs S OSrnZ.o)o2. z s5 m 01 2oS aa 1-sx 1130 Cn-a-s Aw asai2z.O o% d 7-6 O1S-tlS 0k'; 7 j 1SS 1211 crpr PS 2n0.ptIOZ-A 7 Control information: Acceptance criteria Summary of testendpoints: % Mortality. 07t S 20%*/ 7-day LCso > 1 7fO Average weight per initial larvae: o.16b

14rM 4-z NOEC 4

to. Average weight per surviving larvae: 0.16 1 2 0.25 mg/larvae LOEC >1 OI. ChV > Ico/. IC25 I> 1067%

Environmental Testing Solutions, Inc. Pageoc~ Species: Pimephalespromelas Client: IVA I Sequoyah Nuclear Plant - UV-treated Date: 01-tll-oS Survival and Growth Data Day CONTROL 10.98% 22% A B C D E F G H I J K L 0 _ 10 110 10 10 10 10 /6 /o /6 /0 10 _ '0 10 10 IC 10 I 0 10 /0 I0 /0 '0 2 10 0 0lt 10( 6 0 / 10 I)t10 3 -lbIt 1010 _ 0 1010 10 /I/O 16 4 to I_ ) tt o o t I0 I0 l/0 io lo I I l 5 ID 10 10 l0 I0 10 10 la /0 6 lb

1.

1 J _0/ / /0_ t 0 7C 101 0 to 10 t o 'IC10 /0I Jo /0 I0 A - Pan weight (mg) t51_ Cololdst Iy,4 1q. 21?Z IS.,2 I, ff IY.0t 5 1.tt5 51 ;.91S Analyst' : B - Pan + Larvae weight Analyst: Kt a 1p9s 3% 5 \\ \\ v ~ Larvae weight (mg) - A - B ,7.12 i.1 -).C. £-22 l~t .q& 1.0C t,,tb 1.06 (,,.11 (.. tic Weight per Initial number oriarvae(mg) - CInitial number ottarvae 1% O' a' Ia k. A? DI 0* D"I '4 \\, O! t O* Average Percent weight per reduction Initial from control Wub k number or (%) ) larvJe (mg) -.1' -%.,t, 1'. 3 -I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. Pagc3 of6.

• 1.S Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date:

M-ig-OS Survival and Growth Data Day 43.9% 72% 100% M N 0 P R S T U V W X °Io 10 JO 10 10 /0 10 /0 16101 10 __ (O -10 ID tO ICID 10 10 toC l 0 2 10 ro -1 0 to 0 /t /0/0 l /O '0'aJ 3 /0 ) to I( /0 (0o 1_ / I I 0 to I0 . 4 // to {Io 0 Io t0 /b q 0 ZlI10 10 10 5 1D _I tO I I 1O I C/I)IC 6 1 C) I 01 160 / /0 /0

7) I0 10o to 10 1o 10 "I

10I 1.0 '0 A - Pan weight (mg) 6AB't Color de7 IS . 5C{ I,.qq 15. q 1 oq I soi tbi.2's tszk lqtS ts.-3 tq.88 I4. B Pan + Larvae weight N L \\C Analyst: v:LOO\\a' a; a5 3 25 \\;' a Larvac weight (mg) - A -B -cug ,74 1.145 1.,48 1.1* - d 1.8111 1.11Z I-,St ,Ioz (..& J..l q,zl Weight per Initial number A or larvae (mng) 4 CCIInitial numberotbrvae CO <<d% b V 6 U o -b-- Average Percent weight per reduction Initial from control bO O.1 .I.-, 0.1 1 number of (%) Larvae (g). Comment codes: c = clear, d dead, fg fungus, k killed, m = missing, sk sick, sm unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. , t 1.. t! Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated I. I I-I. i... i' I.; i Date: 0711q'OS Survival and Growth Data Day 100% Intake-Y Z AA BB 0 1I O'o 10 lO 1 /0 /0 /L JO 2 16 L (* ()o 3 JD 1 /0 10 .4 to /O {6 / 5l1 10 D10 1 6 lo )O 10 iO 7 7 LO 0t A - Pan weight (mg) i6oto5 . ;oor Ien Illptlon: ge5I 21.I5.ISZO 8 Analys

4z B - Pia + Larvae weight (mg) 0 3

Larvae tweight (mg) - A -B Weight per Initial number oflarvae (mg) t ) -C/Initial number orlarvae te O .\\ a. 0. 0 -) Average Percent weight per reduction Initial from control 66 l0 I z.0% number of (%/6) larvae (mg) .4 Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

• Calculations and data reviewed: 4 Comments:

t I-A b I ' r 4~~~~~~ FCIA~tS^ts 4Sto -s FI-at._t v

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated July 19-26, 2005 Chronic Whole Effluent Toxicity Test (EPA-821-R.02-013, Method 1000.0) Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, snd Statistical Anslyses Client: Test dates: TVAl Seqaltysh Nucler Mlaet. Outrun 101 -UV trtated July 19L.26 200 Revelwed by: rn /, 4, Project number. l99 Not for Oompliance Assessment. Intersal Labodtory QC C _.W"-

• l)

R1 1 1_ __{ A1-fb t 1 n-_t Wogbils-ft M.. wabd Il S _wdgb ill__b M.-AgbiltZ_4 Cifid. t.- _f. s tt _ m f l l. a A d .1 L. _r .d

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-a C.1 d a t S t wai _ _. -sw-A 10 10 14.99 22.11 7.12 0.712 _0.712 Control t 10 tO 16.26 23.67 7.41 0.741 0.761 6.5 0.741 100.0 0.761 6.5 Notsappliuae C 20 to 14.94 22.55 7.61 0.763 0.761 D 10 10 16.24 24.52 8.22 .O2t 0.82t E 10 10 15.22 1 23.20 7.93 0.798 0.79t 10.91Y% F 10 10 14.82 22.10 7.98 0.798 0.746 8.2 0.791 100.0 0.746 8.2 2.0 C 10 10 14.94 21.94 7.00 0.700 0.700 It 10 10 15.42 22.28 6.86 0.686 0.686 I 10 to 14.97 21.97 7.00 0.700 0.700 22% J 10 30 15.17 21.94 6.77 0.677 0.703 7 7 0.677 100.0 0.703 7.7 7.5 K 10 10 16.19 22.75 656 0.66 .6 0.656 L 10 10 1525 23.05 7.30 0.780 0.70 M 10 10 IS.Ot 22.82 7.74 0.774 0.774 41.9% N 1o 10 34.44 2.99 7.4 074t 0.760 2.0 0.743 100.0 0.760 2.0 0.1 0 10 tO 14.44 21.92 7.48 0.747 0.748 10 10 14.94 22.86 7.92 0.792 0.792 72% R 30 10 15.03 22.91 7.88 0.73t 0O77 J 0.7t 97.5 .7 ].1 7 S 30 10 16.2 2357 7.32 0.3272 T 30 9 15.21 22.72 7.531 0.834 075 0.7207 0.720 U 30 l0 14.85 22.03 7.20 0.720 200% v 10 I 0 I 15.31 1 22.19 1 6.88 1 0.683 W I 10 I 10 1 14.85 1 22.19 1 7.31 1 0.731 0.765 13.8 0.720 0.731 0.921 300.0 0.765 13.8 .0.6 to to 14.99 24.20 9.2 0.9213 .t.-.---i.-----q r I P 5 I 1 22.20 j 0706 0.724 8.1 92.5 0.670 8.5 320 15.22 21.13 5.90 0.662 00% talske l 22.20 7.06 0.706 1 22.42 1 7.22 1 0102 0.724 8.1 0.596 0.706 0,72Z 0.654

,2*

0.6i0 8.5 I 2.0 21.40 6.54 7.127 Duinets hISD value: PMSD: Intolwt: Dumell's MSD value: PMSD: 0.1016 33.4 0.0732 9.6 MSD - Minim SiPIfiecant Difference PMSD - Percent Miimum Significant Difference PMSD Is a measure of "est precision. The tMSD h the minimum percent differencebetweeni the control sad treatment that can be declared startstily SInificnt In awhole effluent toxicity test. On veragea siganificant difference occn rOe nionmental Testing Solutions. Inc. chric toxkity tetswban tolicant redue Pmephates owth by 3% fm the control (determined thugh releraee toxiant tosting). Lowet PMSD boud detmined by USEPA (10th peretilk) - 94%. Upper PMSD bound determined by USEPA (90th perventile) - 3 5%. Tbe lower sd Iper bounds were ca hlated bythe USEPA using 20$ lets conducted from 19 labortoties forPiephales powtSb [i chronic re lrcnce toxicant tests. USEPA. 2000. Unde dingad Aeounting or MethodVusabiglq in Whol E nflientTolkityAppcations UndertheNtional Polluta Discharge Elimation ProptL EPA33.R.093. US Environmental Protecon Agen CicIuati. OH.

TVA I Sequoyah Nuclear Plant, Outfall 101 UV-treated July 19-26,2005 Statistical Analyses Larvvi Fbi Griw ud S4 vs l Tmt.7 Day Growth Sum Date: 7/19nco TetID. FpFRCR Smylek M. IVAISaqyaNclrarntruhWIOl..- utreed Eod Datc 7MM2005 Lab ID. EM2vk. Tetg Sol Sample1 7DPc MR .Di.ch-s MonbitdgRepot SapleDate: Protooot IPWHR-EPA121-RP24013 TedSpecies: PP.?hapharsp iss Comment Co.' 1 2 3 4 D-Control 0.7120 0.7410 0.7610 03280 109o 0e7910 0.7910 0.7000 0.6160 22 0.7000 0.6770 0.6560 0.7300 43.9 0.7740 0.7450 0.7410 o.7m 72 0.7920 0.7100 0.7320 0.7510 too 0.7200 0.630 7 o0.o 0.9210 Tru rfu Ustrvutd

1.

t-Ti;hd 1*aaaeuo C"c-% Mini N-Mesm M? x m1in Mar Cv% vn Stft CH"a1 MSD Mea NMda D-Col 0.7605 1.O 0.760 0.7120 0.1210 6A,1 4 0.7605 LOWO 10.9o 0.7455 0.903 0.7455 0.6e60 0.79S0 2.163 4 0.356 2.410 0.1026 0.7479 0.9234 22 0.7033 0.9247 0.7033 0.6560 0.7e00 7.711 4 1.359 2.410 0.3016 0.7479 09r34 43.9 0.79 0.9990 0.759e 0.7450 0.7740 2.023 4 0.016 2410 0.1016 0.7479 0.23J 72 0.7658 1.0069 0.7633 07320 0.7920 3.300 4 .0.125 2410 0.1016 0.7479 0.9134 0oo 0.7650 1.0O59 0.7650 0.6980 0.9210 13.302 4 .0.107 2.410 0.1016 0.7479 0.92u AsaitleryTest StOi2 Crl Stk Kurt sSpr-sTe tes l v detb (p>l) 0.920691669 0.14 1.196220214 1.926649961 tv >Tistkdkat aequal vinca (p- 0.10 9.362381472 I5.0u627224

• tterbt(I.ttL.O3)

NOtC LOEC CoV TU MSDv MSDp MeSS MSt FPrb d4 DuinnauaTest 100 XO1 I 0.101359139 0.133542359 0.002307342 0.003531611 0.665465117 531s Thbesut vn D-Contmt sur latlrpolad.. (200 Roamplu) pan SD 9S%C4Exp) Sk.' ICOS >100 ICto >100 ICts >100 1.0 IC20 >100 0.9 1025 >100 IC00 >100 0.8 C00 >100 0.7 0.6 0.5 I0.4 0.0.3 0.2 0.1 0.0 0 20 40 60 ea 100 120 0.9. 0.7........ 14 l 0.4 r-2 O.2

A.,

0 sqn_07.19-05data

TVA / Sequoyah Nuclear Plant, Intake UV-treated July 19-26,2005 Statistical Analyses Lavsl Fsla Cm.* ad SarvsiTw.7 Day GrCtb Sut Date: 76194o5 TMtID: 1 pFRM Saml ID,. TVA/Sequoyah Nocker flank Intar - Wh trated FMA Dec 7/26/2005 Lhb ID 5

r. TatingsOL Sample Ty7p:

DMR-Dbchap Monk-kg P R-Iot Sample Ddtcc hotocal FWCR.-EPA421-R-O2.M11 Ten Specimc PP-hmepks~p pVomelu -Yme.% 1 2 3 £ D-Caatr1 0.7120 0.7410 0.7610 OAU2O loo 0.5960 0.7060 0.7220 0.6540 Treasarw: Ustmrsfrnd 1-Taled jk Cse-.% Mum NiMsa Mfefsa Min aM CV%/ N tsht Icuj MSD Mean NMes D-Ctrol 0.7605 .O00O 0.7605 0.7120 0.230 6.481 4 0.7605 l.o000

• 100 0.6695 0.8103 0.6695 0.5960 0.7220

.507 4 2.416 1.943 0.0732 0.6695 03S03 Avxlilsry sfetistc Skm Karl Sbapko-Wks Test lodwat doubusm (p > 0A1) 0.96156 0.749 .003401617.1.17375 F-Ted iedkates squal mn m (p- 0.32) 133502543 47.46722794 Hypetbei Test (1abi..15) MSDv MSDp MSDB MIdE N-rb d Homos ictTe ti ddra mees 0.07318166 0.09622135 0.016562 0.002S36667 0.052124374 1.6 Tmrnaasta vs D-Conltol U.Ammr 1es -mtespel (200 Ressple) POWt P SD 9s%amxp) Skm ICOS* 41.716 ICI0 83.571 ICis >100 1.0 IC20 >100 IC25 >100 IC40 >100 0.° 1C50 >100 0.7

• gmda IC estmt es tasn thewat ebt 60.6

&0.5 0.4 0.3 02 0.0 0 20 40 60 80 100 120 DosD % DessReepme Plt 0.8 0.7 .c 0.6 0.5 0r 0.4 .02 0.1

• 14s8, 0.06 "lI oulgrdtlcsnrce CII sqw_07.19-05data

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pirnephalespromelas Daily Chemical Analyses TVA/ Sequoysh Nuckar Plant, Outfall 101 -Non-treated Client: Test dates: July 19-26, 200S Project number: 1991 Reviewedby t 1 Concentration Parameter r pil (SU) DO (mg/L) Conduclqty (pmhos/cm) Control Alkalinity (mg/L CaCO1 ) Hardness (mgtL CoCO,) Temperature (C) 25.01 24.51 24.81 24.61 24.71 24.31 24.81 24.31 24.71 24.2 7l (SU) 7.81 7.5 1 7.75 7.79 7.90 7.65 7.81 7.70 7.89 7.64 7.87 7.70 7.93 7.62 10.98% DO (mZ/L) 7.7 6.9 7.6 7.2 7.5 7.1 7.7 7.3 7.8 6.7 7.5 7.3 7.4 7.0 Conductivity Oimhos/cm) 26t8 287 275 296 270 -273 276 Temperature (C) 25.1 24.6 24.7 24.6 24.8 24.2 24.7 24.2 24.7 24.2 25.0 24.6 25.1 24.2 pI, (SU) 7.79 7.46 7.73 7.74 7.88 7.62 7.79 7.73 7.87 7.62 7.87 7.68 7.90 7.54 22% DO (mgL) 7.7 6.8 7.6 7.0 7.6 7.2 7.5 7.4 7.8 6.7 7.4 7.3 7.8 6.6 Conductivity (oumhoscm) 255 274 260 236 256 258 264 Temperature (C) 25.1 24.4 24.7 24.7 24.8 24.5 24.7 24.3 24.8 24.3 25.0 246 25.2 24.2 p11 (SU) 7.74 7.47 7.68 7.71 7.85 7.62 7.75 7.70 7.83 7.65 7.84 7.71 7.86 7.55 43.9% DO (mgfL) 7.8 6.9 7.6 7.0 7.6 7.2 7.4 7.3 7.9 7.0 7.3 7.4 7.7 6.8 .Conductivity (pmios/cm) 225 241 228 232 224 226 230 _Temperature (C) 25.2 24.3 24.9 24.6 24.9 24.6 24.9 24.3 24.8 24.1 25.1 24.5 25.1 24.3 n1l (SU) 76 7381 7.63 7701 7.8( 7.561 7.711 7.691 7.781 7.61 7.711 7.8 7.53 72% DO (mr/L) j

7.

6.8 7.6 [ 7 .1 7.5 7.1 7.5 7.5' 8.0 6.9 7.41 7.51 7.8 68 Conductivity (pmhos/cm) 1 83 ~ ~197 18 ~ 191183 J.4 185 ~ ~1896i Temperature (C) I1 (SU) 4- )O (mgtL) 4

onductlyltv (umhos/cm)

Z v 100% Ukallnity (mZ1L CaCO,) lrdness (mW/L CaCO) 4-rotal Residual Chlorine (mPIL) remnerature (C a..- 7.62 7.34 7.59 7.70 7.77 7.57 7.65 7.66 7.74 7.60 7.75 7.67 7.8 6.6 7.6 7.1 7.5 7.0 7.6 7.3 7.9 6.9 7.3 7.2 1 10143 1 4 -13913. <D.IC ~<D.10 01 25.3 24.5 24.9 24.4 24.9 24.4 25.0 24.1 24.9 24.3 25.1 24.6 7.64 7.39 7.60 7.71 7.80 7.58

7. 7.

7.66 7.731 7.62 7.75 7.67 7.8 6.9 7 7. 7.2 7.5 7.3 7.5 7.3 7.9 6.9 7.3 7.5 42 151 143 8 140 140 25.11 24.3 24.7 46l

2.

24.3 2.8 24.3 491

2.

25.0 l 2. pll (SU) )O (mg/L) Conductivity (tmhos/cm) 100% Intake Alkalinity (meiL CaCO) Hardness (mg/L CaCO,) Total Residual Chlorine (mg/L) Temperature ('C)

EnAronmental Testine Solutions, Inc. Page 5 of 6 Envrometa Tetnouin. _nc Paeo Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant - Non-treated Daily Chemistry: Date: G1-119-5 Dav 0 i 2 Analyst A A6 Concentration Parameter 1JS i 'QK Ww. 1 4 CONTROL pH (S.U.) '1.-0 -7, jl lTs I DO (mg/L) r 1

L1 q

Conductivity 2 (iimhos/cm)_____ Alkalinity (mng CaCOA) Hardness (mg CaCOAI) Temperature 1C) 25.0 - 4.5 lto %( --I pH (S.U.) -1. B I 10.98% DO (mglL) 7 Conductivity (jimhos/cm) _____a Temperature (°C)

• ZS. I 724.b 2LI.1

?AL le '. 4 pH (S.U.) 9 ? YG 22% DO (mg/L) 1.1 C L Conductivity (pmfhos/cm) Temperature (C) ZS.1 21.4 2L.-I1. 1 I. .5 p1H (S.U.) '1.4 ?.. 43.9% DO (mg/L) Conductivity ZZ' (pimhos/cm)__ Temperature(C) 2S.Z 2c1. 3 .5-75 *6 b

4.

• Z.Z4 4 pH (S.U.)

-1. ta 39.38 V.&'3 ':I9o "4.<g 7-4M 72% DO (mg/L) 1._ 9_.C* Conductivity I (gunhos/cm ) I__ __ Temperature CC) Z IO.5 2 A, Lt 7j%(.j 24.* PR (S.U.1 - bi r,3 .?@S9 7.67-L? 100% DO(mgfL) O Conductivity '4.3 4 Alkalinity (mg CaCOA/L) S A b Hardness (mg C2CO)L TR chlorine (O. 10 Temperature (C) ZS.-3 2q.5 -I' pEI (S.U-.) .It 3 01 i.?tL K-Sf 100% Intake DO (mglL) i.g

1.

9* Conductivity (pmhos.?cm) LI_______ Alkalinity (mg CaCOAL) __O N __2 Hardness (mg CaCO/L) TRch.10 loo i (emgCL) I ______Temperaturt( CC) 2 I 24.3 +/-J Initial Final Initlal I Final nitlal Final d a - L .a IL

Environmental Testing Solutions, Inc. Page 6 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - Non-treated Date: 01-1i-OS 3 4 6 Analst 4 I S cetin-Parameter i f CN,`ROL pH (S.U.) 1jL 3 !1. 97 %5 7 715. III .<{ w Z 7e DO (mg/L) 9-4 A.O) -wit ( 'S .-7 3 Conductivity ~ ):/ (pnmhos/cm) Alkalinity (mg CaCOA/L) Hardness (mg CaCO)L) Temperahure (°C) 79(( * .7 3A. Q 4.9lZl.l i. pH (S.U.) 7 -7.

7 10.98%

DO (mg/L) 5 l ).3 8 IV' Conductivity__ 7o 13_ ____Tcmpenaturc ('C) 2q-1 Z{2 ^2.l 7.~.'.Z Z5. w o 2tIb,.52 (;imhoscm) Z3 5 .Temperature (°C)

24.

7-4.7- '2, XZ.7 _z5 x5. 0 ZqA0 ss pH (S.U.) 1

• ? S7;

.7. w - 170

7. sY 22%

DO (mg/L) S Conductivity a5 (jimhos/cm) 13 rS-Temperature (°C) 7ZA.1 14 243 3z &.S.2 25 .4.2. pH (S.U.) 0 ?_ -7' 43.9% DO (mglL) 2.-0 Z..12.q Conductivity (,umhoslcm) I83__ 2-Temperature (C) °Z(4 .9 >3 ZLq i Fr

25. I

-Zs. 72% DO (mg/L) C A 7 Conductivity 191 (Rxmhos/cmn) Temperature ('C) A3 A l 2z-- 243-Z 2S. I 2ZG 2S pH (S.U.) L 6 n 1 l - t

7.

G5 100% DO (mg/L)

4.

y Conductivity ~,- (pimhos/cm) I L°l Alkalinity (mg CaCO9L) M 50 Hardness (mg CaCIL) TR Chlorine (mg/L) '0Ix3 .fr _i Temperature (C) ZS.O .9. l 3 Z5 0___ 2S pH (S.U.) ________2 Conductivity ~ (mg CaCO4f) Hardness (mg CaCO3/L) VMS____ TR chlorine (mg/L) ~ Vtt~<0.10 V.4t ____Temperature rc) 3V4.t q .13. q jj I 73

6.

2.1, 2.2. Initial I Final Initial I Final Initial Final Initial Final  - i - i -

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Daily Chemical Analyses TVA / SequoyAh Nuclear Plant, Outfall 101 - Non-treated Client: Test dates: July 19-26, 2005 Project number: 1991 Reviewe bydI V 2V ~ ~ ~ ~ Concentration Paraneter I I. nil (SU) - DO (mrfL) Cond-divlft tmn/m Control i Alkalinity (mgtL CCOC) t l Hardness (rnmgL CaCO1) .0-Ad-an 4-l_____ emperaturel I L4., I.3. I L1.81 L:).L ZliD0 Z1U Iii. L3. I L'.D hO. L1. I L~u L3.A Zh.51 pit (SU) 7.81 7.77 7.751 7.90 7.90 7.79 -781 7.86 7.89 7.91 7.87 7.88 7.93 7.84 10.98% DO (mwL) 7.7 7.6 7.6 7.5 7 7.5 7.7 76 78 7.3 75 7.8 7.4 7.5 Conductiviq (pmhos/cm) 268 287 275 296 270 _2 271 _26 Temperature (C) 24.9 25.0 25.1 25.3 24.7 24.8

24. 9 25.0 24.6 24.8 250.

24.7 25.2 24.7 p1 (SU) 7.79 7.76 7.73 7.84 7.88 7.78 7.79 7.87 7.87 7.90 7.87 7.89 7.90 7.83 22% DO (mgtL) 7.7 7.6 76 7.5 7.6 7.6 7.5 78 7.8 7.4 7.4 7.8 7.8 8.0 Conductivity (umhos/cm) 255 274 260 236 256 l__2126 Temperature (C) 24.9 2501

25.

25.3 24.7 24 24.9 25 24.6 24.8 25.0 24.9 25.1 24.7 pil (SU) 7.74 7.68 7.81 7.85 7.75 785 7.83 7.89 7.84 7.88 7.86 7.82 43.9 i DO (mE/L) 7.8 76 7.6 7.4 7.6 76 7.4 7.7 79 7.4 7.3 79 7.7 8.1 Conductivity (pimhos/cm) 225 241 228_32 224 226 230 Temperature (,C) 24.8 25.1 25.1 25.2 24.8 248 25.0 25.0 24.6 24.9 25.2 24.7 25.3 24.9 p1i (SU) 7.68 7.72 7.63 7.81 7.80 7.73 7.71 7.84 7.78 7.87 7.80 7.85 7.80 7.80 DO (mgIL) 7.8 7.6 7.6 7.4 7.5 7 7.5 7.7 8.0 7.4 7.4 8.1 7.8 7.7 Conductivity (umhoslcm) 183 19 184 191 183 185 189 Termperature (C) 24.9 24.8 25.3 25.3 24.9 24.8 25.2 25.0 24.7 24.9 25.2

24.

25.2 24.8 pit (SU) 7.62 7.67 7.59 7.79 7.77 7.69 7.65 7.81 7.74 7.82 7.75 7.8 7.70 7.77 DO (mZ(L) 7.8 7.6. 7.6 7.5 7.6 7.6 7.9 7.9 7.5 7.3 8.1 7.8 7.7 Conductivitz (pmhostem) 143 _S 143 147 139 13 143 100% Alkalinity (mg/L CsCO,) 50 50 50 alardness (mgtL CaCO) 56 62 56 Total Residual Chlorine (mg/L) <0.10. <0.10 Temperature CC) 25.1 25.1 25.51 25.31 24.9 24.9 25.21 24.8 24.8 24.71 25.1 24.81 25.21 24. pI[ (SU) 7.64 7.641 7.60i 7.791 7.80 7.70 7.681 7.78 7.73 7.831 7.75 7.79 7.69 7.74 DO (me/L) 7.8 J 7.51 7.51 7A4 7.5 7.5 7.51 7.6 7.91 7.4 7.3 7.5 7.81 7.5 Conductivity (pmhostVm) 142 15 1 148 140 140 143 100% Intake Alkalinity (metL CaCO,) 52 52 51 Hardness (mg/L CaCO3) 70 6 56 Total Residual Chlorine (mg/L) <0.10 1 <0.10 Tempersture (IC) 25.0 25.11 24.91 25.31 24.7 24.91 25.01 25.1 24.7 24.91 25.11 25.01 25.2 24.7

Environmental Testing Solutions, Inc. Page 6 of 7 Species: Ceriodaphnia dubia Client: Sequovah Nuclear Plant - Non-treated Daily Chemistry. Date: cM-I9 -S5 Analyst K; fT Concentration Parameter 0O

s.

CONTROL pH (S.U.) go fs i. DO (mg/L) Conductivity (g+/-mhos/cm) 2f Alkalinity (mg CaCO/1 L) b\\ Hardness (mg CaCO "I4 Temperature (°C) 2LA.1 ZS. A. Z5-

24.

a.t) pH (S.U.) j2, pZ .f-b 72. 10.98% DO (mWl) z Conductivity (uim~hoslcm ) _I__ Temperature (°C) 21.: z5, 2S.1 l 'S. pH (S.U.) 7.i7 SC

9. -3

?. S S'Y st? 22% DO (mg/l.)

G v

2 Conductivity9I-Temperature (C) 24Ai -Z - 2.1 1S 3. -2t4.- I pH (S.U.) 4' 43.90/6 D (jg/L) s .Y* ?. Conductivity (gimhos/cm) Temperature (C) 7z.L.& Z-.1 2S. 1 pH (S.U.) 9, r6 72% DO(mg/L) Gj 16 5 Conductivity (P rnh os./cm ) Temperature (°C) 'ZS.

5. -

2S.5 ZS.3 21 '.4. pH (S.U.)

Z

.9. 2.. ..2.S1.. 100% DO (mglL) Conductivity (+/-Tnhostcm) 5 - Alkalinity (mg CaCOA) i i ny3 Hardness 10 b (mg CaCO3/L)l w" TR chlorine C) <0 Ci (mp-L) Temperature (0) Is-'. -4 pH (S.U.) qeC

.(0V-~1 T

100% Intake DO (mg/L) Conductivity OIznhoslcm) Alkalinity (mg CaCO/1L) 62 Hardness (mg CaCO]/L) 1 TR chlorine ./ (mziL) <0.1 - i Temperature ( 0C) .7Z5' .2~. IIntilD A Final I Initial B Flnal I Initlal Final InItial I Finil I InitiI II In1' Initial Final

Environmental Testing Solutions. Inc. Page 7 of 7 Species: Ceriodaphnia dubia Client: Seauovah Nuclear Plant - Non-treated Date: C1 OS Anals R7 1*s ?W_ El E Concen-Parameter 'W# tra tio n CONTROL pH (S.U.)

7. 9 I 9 8 7

r-DO (mg/L) A Wn5. ij R 'i5 - 1 9-GsL. Ternperature (eC) Z .~ n z; ESZA sZ5.' Zs.o

25. HZ E

pH(S.U.) -{ 7; O,_£ -;1 7 g ?q

~

Conductivity 2 w (jimhos/cm) 9 Temperature (CC

4.

j 2s.0 244... Zl.E 25.0O Z

• 2.5.2 ZVl.1 l pH (S.U.)

-J: _7 ^ 7 ^.t -%7^ S -1 22% DO(m .7L) Temperature (C) Z4l 't 2'.L 2'4.R 2S.0 q pH (S.U.) Y.-3 7 10.98% DO (mg/L) Conductivity .a. .(Comhdos/cimi) as

70; 1

Temperature (C) _s.O _44.o M.. 5 2S. 2q -1.5.3 7-_4.1 Tepeatr (a 2 -14 -Z, -249 pH (S.U.) -1i M 1.i 9 -7 -7

7.

n 72% DO (mg/L)

7. 4.

11 94 Y I. W, - s} ,6. ,-_i I 11s. _L al l}'I-vI_-9\\s Conductivity ( umhos/cm) 'IV? 14 Temperature (C). z2S. Z5, 0 22 L -1 2 -5 z 7Z

72. T

-S. -.

2.

t pH (S.U.) -7l . 14 1 -F 7 -J-.- 100% DO (mg/L) ?- -7. S j. ./. Conductivity (mgCaCOl) (mg CaCO 3fL) 5 TR Chlorine (mg/L) <.O. 1 0 Temperature(°C) s2 5.2.. {+j.- .1 ZS. t 2q.S _S2. 7 _L4 t pH (S.U.) c 6 J '7 -7.1 I 7. 2.27 .1 I-100% DO (mg/L) L < 7 . 3 (Pmbos1cm) /4fg (4 In, t 14 Alkalinity (mg CaCO3/L) guy."' Hardness M; (mg CaCO3IL) M ~M IEI TR chlorine (mg/L) .T@ l O

J.

_Temperature(°C) 2S.0 2S 1 2 i1 I. Z.. 2t'I Initial Flnal I.W.1a Finsl I Inlttsl I Flnal FIntial Final 11

a...

• -^r-
• ^z

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pinmephatespromelas Daily Chemical Analyses TVA / Sequoyah Nuclear Plant, Outfall 101 -UV-Treated Client: Test dates: July 19-26, 2005 Project number: 1991 Revltwedbyc Al Concentration P. ramet r Day 0 Di I Day 2Day 3 Day 4 Div 5 Day 6 Concentration __rmeer_ Initial D Final Initial I Final Initial Final Initial Final Initialf Final Initial iz Final Initial Final nil (SU) 7.82 7.54 7.75 7.79 7.91 766 7.80 7.74 7.87 7.60 7.87 7.72 7.86 76 DO (mdiL) 7.7 7.0 7.7 7.5 7.5 76 7.7 74 7.8 6.8 7.6 7.4

7.

7. Control Conductivity (pmhos/cm) 28 281 276 29E 284 3281 Temperature (C) 24.9 24.5 24.8 24.7 24.8 242 24.9 24.8 24.5 24.8 24.6 25.0 24 pit (SU) 7.81 7.53 7.74 7.77 7.91 7.62 7.79 7.71 7.88 7.61 7.87 7.69 7.88 76 10.98% DO (njL) 7.8 7.0 7.7 7.6 7.5 7.1 7.6 7.3 7.7 6.7 7.5 7.1 7.7 70 Conductivity (umhostcm) 27; 290 1 276 281 271 278 280 Temperature (C) 25.1 24.4 24.8 24.5 24.8 242 25.1 24.2 24.8 24.3 24.9 24.6 25.1 241 pil (SU) 7.79 7.46 7.72 7.75 7.89 7.63 7.78 7.70 7.87 S 7.60 7.88 7.72 788 763 22 D DO(mJ/L) 7.8 6.8 7.7 7.6 7.5 7.2 76 7.3 7.8 66 7.6 7.2 7.7 69 C Conductivity (pmhos/cm) 257 275 261 237 22 2564 mTeperature ("C) 25.2 24.4 249 24.6 247 243 250 24.2

24.

246 25.2 24.6 25.1 244 pt (SU) 7.75 7.43 7.70

7. 8 758 7.75 7.71 7.8 753 7.86 7.68 7.85 758 DO (mg/L) 7.8 6.7

7.

7.6 7.6 7.3 7.7 7.3 7.7 64 7.6 7.3 7.7 67 4.9 Conductivity (urmhos/cm) 224 24i 232 259 227 231 235 Temperature ('C) 25.2 24.4 25.0

24. 8 242 25.2 243 24.9 243 25.2 24.6 25.2 24.4 pI (SU) 7.71 7.46 7.66 7.70 7.84 759 7.72 7.70 780 7.55 7.82 7.71 7.83 7.62 DO (mg/L) 7.8 6.9 7.7 7.6 7.6 72 7.6 73 7

6.8 7.4 7.2 7.6 7.1 72 Conductivity (utmhos/cm) 185 20 188 228 186 185 190 Temperature(2C) 253 25.1 24.5 24.8 241 25.2 242 249 244 25.3 24.5 25.2 243 pitl (SU) 7.64 7541 75 7.65 7.80 7.54 7.66 7.69 7.75 73 7.76 7.67 7.77 753 DO (mg/L)

7.

69 7.7 7.6 7.6 7.2 7.6 74 7.7 67 7.5 7.2 7.7 69 100% Conductivity (pmhos/cm) 144 151 143 _18 141 142 139 Temperature (C)

25.

243 25.1 24.5 24.9 24.1

25.

242 25.01 24.4 25.3 24.7 25.3 242 pH (SU) 7.69 7.43 7.62 7.66 7.82 7.57 7.66 7.70 7.75 759 7.77 7.76 757 750 DO (mr/L) 7.8 7.0 7.7 7.1 7.7 7.4 7.7 75 7.8 6.9 7.7 7.4 76 69 100% I ntdke nductivity (pmhosfcm) 14-4 151 t 141 139 9 14 142 _ Ttemperature (C) 25.2 24.2 24.9 24.4 24.7 24.1 25.1 24.2 24.9 244 25.2 24.6 252 244

+i_ -sf Environmental Testing Solutions, Inc. Page 5 of 6 Species: Pimephalespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: 1_ _ -_ _° Daily Chemistry: Analyst 4 Y Concentration Parameter d 9 CONTROL pH (S.U.) DO(mg/L) )5TA .O Conductivity (gmuhas/cm) Temperature (c) ZI i S.5

• 82'4L pH (S.U.)

?

.?5 10.98%

DO (mg/L) l_8._6r Conductivity (pim hos/cm)__ Temperature (QC) z5 I 2. T 2A.5 2 L. 2A. Z pHl (S.U.) e 6 -;7 .;,qC 22'h DO (mg/L) _G8___ Conductivity (gmnhos/cm) TemperatureCC) (Q .2 2.4. I.I b L'1 1.3 pH (S.U.) ___43______ 9 9 5 43.9% DO (mg/L)

7.

.3 Conductivity (pimhosicm) / _._i Temperature (C) -Zs.L-3 -*q tA Ze 2 1 ? 2I P'H (S.U.) .Y . ff ?, I.& a q.s y 72% DO (mg/L) Conductivity (izmhos/cm) _/43 Temperature(C) ( ZS-3 2.2-3 I I Z.'i7 pH (S.U.) I__ 100% DO (mg/'L) Conductivity, Temperature___(_ 2.S.3 2'I. 25.1 24.1z4S~ -q.i pH (S.U.) 1 100% Intake DO (mg/L) ?j .O I> Conductivity I (pmhporusrcm) S.. 2 /1/ ITemperature(°C I2'S.' 2-7-Z. zq. V-r ri. l-1 Initial Final Initlal Final Initial Final a i -L .aa

Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page6of'6 Species: Pimephaiespromelas Client: TVA I Sequoyah Nuclear Plant - UV-treated Date: O-l-i C5 Anal-At Concen-Parameter l tratloii "-,________W M CONTROL pH(S.U.) -1. TZ -7.6& 0 1 W, Q. 2-I . R(. DO (mglL) l C ConductIvity (pmhos/cm) ______4__ Temperature(C) 3*% Z9 4 Zt. 2zq lL 2'4.(. 25.0 3 pH (S.U.) C 10.98% DO (mglL) Conductivity 2 i Temperature (°C)

s.

22. L.

z.& 213.9 --. 1. b Z5o \\ pH(S.U.) __1__ o 0L i 22% DO (mg/L) . 6

,;; +i: *- -

Conductivity (pglmho~s/cimi) C t S ) da5l Temperature (°C) .O

2.

224 (

2.

S. Z . (a 2S \\ pH (S.U.) I 7.__ 43.9% DO (mg/L) Conuctivi~ty Temperature CC) 2s*

75. Z.

.a. pH (S.U.) = 2-0 7 -SO

7. X 5 i7.

72% DO (mgL) Conductivity 2 I

• flr (pmhos/cm)

~ ~ Temperature (°C) ZS.L . 2. 7A q 241-1 25-3 214. S ZS*2_. zc4 3 pH (S.U.) c;

71.

6 9 i.-7 r i t 100% DO(mg/L) 00; ConductivitY i9S 19 I Temperature (C) -s. 2S 2A.2 Zs.0 q 37 7.3 pH (S.U.) L7.7 O 7.75 7 100% DO (mglL) 5 / .G Intake 1-L/C Conductivity /3. g.i s: (_mhos/cm)

_L_

ITemperature 17C) ... 3.L2q.9 q1 25.2

2. 0 ZS.2~

Initial Final Initial Final TnItlal Final I Initial Final

s.. I, I I. I. I I I I Page 29 Page I Of,3.3 Environmental TestinZ Soluttions, Inc. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO39L Analyst l II t I Z I Date analyzed toq-1-45 I Titrate samples to pH = 4.50 S.U. Titrant normalt and multilier determination: pH of Normality Normality (N) of H:S0 4 pH Factor or Multiplier Delonized Titrant check Begin End Total (5 ml ?a CO3 x 0.05)/E (iVx 50000)1 100 nml sample water rererence standard ml ml ml =0.25/E - IV X 500 -4.5 S.U. number number (E) (accept2ble range - 0.013 -0.022) -6 q I IARI-% 1rj~j:- o.1 1,71 1,2.

0.

( Pi Io 5 Lab oratory control standard: Reference ITrue value Sample 7 lAlkaliity (MV)' % RS = *1V/ITV sl10 standard number (TV) volume Begin End Total Multiplier (Ing C2CO-/L) (acceptable range (tng CaCO3t1) (ml) ml.ml ml -90 to 110%) VAS2s100 100 12.o '2l j1 1b I 102Z7. -II6 Duplicate sam le precision: Sample 2Zl j5.0.5). Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO)IL) I(S - D) I[(S+DY211 x 100 number (ml) ml ml ml (acceptable range - t 10%) ON-oS5 SsJ (h -tO Io. S d. Duplicate 10

2. 9.0 7h.2 3,2Z D

3 .c .~2~ 2 D Mkatrix spike recovery Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO;dL) (mg CaCO2/L) (ml) ml ml ml SSASE 5-0 Ica 25.0 3.{ 1 8-0 1.4 03 Samplealkalinity(B) lryeasured spike value (MV) % R MIV /SV 100 (mE CaCOA.l) NIV - A - B (acceptable range l l (mg CaCOVL1) -75 to 125N.)

33

6.

1001. Sample measurements: Sapevolume Begin End Total Alkalinity Samplenumber SamplelD (ml) ml ml ml Multiplier (mgCaCO,/L) 0 -4I mOS A Ml4S t-b j..oq

13. I

, to04O 0-4-IB-09~~* 1 l4o 4q I r 0' -oA 6Ca+ I 14*2-18.1 I -_ 1 1 1 0-1-3 -3OS 6 Wm____ Jq , ~z 14. l 5cc txre- _2l Z l ?A42, _11_.4 1 ll X' A ty c b Z.1 a1. S l4 t S. z 1l m ls cr. t mo j ;.- 1 1t2.o 1 I.1 i ii_

1.

z3~ s o.' t1 i-b Reviewed by: e ir i Date reviewed: -I~o;o

Page (.%) Page 2_ of 3 Environmental Testing Solutions. Inc. I Alkalinity (EPA Mlethod 310.1) Matrix: Water, MDL = 1.0 mg CaCO/L Analyst Date analyzed 0L - 1-o Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH Normality (jN) of H2SO pH Factor or Multiplier Deionized Titrant check Begin End Tota-iC x 0.05)IE = (IN X 50000) 100 ml sample water reference standnrd ml ml ml =.81 Vx S00 -4.5 S.U. number number (E) (acceptable range - 0.018 - 0.022) 4 Laboratory control standard. Reference True value Sample I Alkalinity (MV) % RS -lV /TVx 100 standard number cm volume Begin End Total Multiplier (mg CaCO31L) (acceptIble nnge (mg CaCO/L) (ml) ml ml ml - 90 to 110%) lJ J;Gsb As 100 100 13.e8 z3m q-6 JO.q bo ( 02 1 Dup1licate sa nle precision:- s Sample Alkalinity %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOA.L) J(S -D) /[(S+Dy211 I 100 number C9-ll-os (ml) ml ml ml (acceptable range -

• 10%)

.f-p dh6-23.Lp 31.51.co i3c, [ Io.L. S 2 0 [ Duplicate O.. 1?." D 2,o Mfatrixv spike recover :_ Reference Spike value Sample '~Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (fig CaCO3IL) (mg CaCOjL) (ml) ml nml niml I/8ssagb l too 50 0.0 l 1.t4 n4AJ L24 lO.l 3(io Sample alkalinity (B) Measured spike value (NM) % R - MV / SV x 100 (mg CaCOjlL) MV - A - B (acceptable range l (mg CaCOj/L) 75 to 125%) I Z(0c tOcj1 100'1. Sample measurements: Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mE CaCOV/L) 0603Xo 17-1 Oaa ja I Q 1qwq 12S4 15 51CO) 10.4 I I lOs l 1 l 22.? 1 1 l~ l l (oZO bSlYi lIoe'. ___3i 3 j.j I L5A(o b6b-4l2.03 C.Lcew L I loc) &1iIq 7(-t___ ml r4)lO. C 3 l S l lO l l q _O5___ ___ z '12.1 r c.OI _a _._5 .3 J I l il i.. L l l I IQ5O91Z£ F~x~o I_ I -,%I. I I I O5391q.09

2.

18~ 2G }17.1 .~2Z1~ 1_ 1( Reviewed by: Da\\ reew Q1t3

• Date reviewed: I e I-Oz 1

Page () I Page 3 ofI I Environmental Testing Solutions, Inc. Alkalinity (EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst KEj)y-Date analyzed O3-Titrate samples to pH = 4.50 S.U. Titrant normalty and multiper determination: Normality (N) of H.SO4 pH Factor or Multiplier Deionized Titrant check Begii ml NazCO3 x.05YE

• (Nx 50000)1100 ml sample water reference standard ml 0

ml mlZ E= 0 -4.5 S.U. number number (E) (2cccptable range 0.01 3 -0.022) Laboratory control standard: Reference True value Sample Alkalinity(V) (M %RS -vITV x 100 standard number (Tc) volume Begin End Total Multiplier (mg C2COjL) (acceptable range (mg CaCOV ) (ml) ml ml ml -90to 110%) I 14-S

?5t 100 100 1&0S 14@(OL 9 j.9 -

10.QtI) O9 D licatepsam lc recision: Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCOIL) ((S-D)l[(S+D)1211 x 100 number (ml) ml ml ml (acceptable range - 10%) oT oI I 10 D, oo.1 04 s L .,\\ to.-( Duplicate 4 p, IO.q 10.q D .2-Afatric spike recovery: [ Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total Multiplier (mtg CaCOjL) (mg CaCO)L)l (ml) ml ml ml l ,) Aas 1 I00 6A 5.(-o .2 IO-%q 51 l Sample alkalinity (B) Measured spike value (MIV) % R -IV ISV x 1OO (mg CaCO 3/L) MhV - A - B (acceptable range I (Mg C2COlL) 75 to 125%) .4.2 '4q15 CRqhO,?. Sample measurements-Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml I ml ml Multiplier (me CaCOL) O l)q 0 3 I 3 i l.o b 82

• Z l2.1 l06?)O-ll l

2 153 CM 9 O bs>0 n3 1 q DT i 5 6 < }z. 3,0 svs.OL 3 S 2 3.3 .3 0 1-loVlOY+.o.W I l_ &.I 14q.(O 66 Be l l I3

z.

.32.c,.

10. rQ

,r IPeca. tl-132e4 134.S _____JDacPPwd lo zO Reviewed by: De re v Datereviewed: I 0&-07-C6-]

1-0.i I.,-~ I ;.I. P e 1

• Environmental Testing Solutions, Inc.

Alkalinity (EPA Method 310.1) Matrix: Water, UDL = 1.0 mg CaCO/IL Analyst Date analyzed 0 Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H1S0 4 pH Factor or Multiplier Deionized Titrant check Begin End Total (5 ml NasCOj x 0.05)/E (IVx 50000)1 100 ml sample water reference standard ml ml ml 0.25/E -NsX500 4.5 S.U. number number ( (acceptable ranage - 0.0t8 - 0.0'2) t/;7 1A1918Y IAJR15'7 O.O 1*7. 1 2 4 O./°le 7. 9 JJ 9 Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS-MhV/TVs 100 standard number (TV) volume Begin End Total Multiplier (mg CaCOJL) (acceptable ranne (me CSCOVLI (ml) ml ml ml 9*goto 110%) jg1s 00 1* l / 6 2.7 i. .9 .77 999 0 D uplicate sa m ple precision:__ Sample Alkalinity %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOjIL) ((S - D) Il(S+DY21I x 100 number (ml) ml ml ml (acceptable rnnge- *10%) 07.18.05 MUs b 1/LO /,O 48.7 -O elj-Duplicate D. a 3 j o Matrix spike recovery: Reference Spike value Sample Spike alkalinity (A) standard number (SV) volume Begin End Total MultiplIer (m" CaCOVL) (mg CaCOV/L) (ml) ml ml ml jjV5S Q5 e-el /070 ffq. 13-0 1II glo /IoI Samplealkalinlty (B) Mcasured spike value (MV) % R - MV I SV x 100 (mg CaCO,/L) I V-A - B (2cceptable range I (mtg CaCO,/L) 75 to 125%) Sample measurements: i Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOIL)0 07.I1.305 M h/ sW o0A / O o.o C.o I.0 q 3 5 lo5<nz m. O -ro-.3 l Io lG.O l/o.7 40l § l lOSI2 1 QI0 l 4.2 l Osc Ok -lron l job 115-.152 4.7 .77 lo 4ON Iorj f lot t oo -.20-al< l* J.

1.

1 rjai5O\\ 5e1.dtJ; I IO (00) O1a45.;13 51.o I5 C.Sfl= /06 3o.3 I .*0 O ZiZa.c q C) (0 to0 s3 50 c)SCI-LO. q sc5 J IN4 /coo

4.

1f.9 Reviewed by: e e d l Date reviewed: I Ok02 0s I

I' l' Environmental Testina Solutions. Inc. 4........ Page a o Page.. Q of Alkalinity ,,i(EPA Method 310.1) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst L i_ l Date analyzed 01.j714 0 Titrate samples to pH = 4.50 S.U. Titrant normality and multiplier determination: pH of Normality Normality (N) of H:SO, pH Factor or imultiplier

Delon, Titrant check Begin End Total (5 ml NalCO% x 0.05)/E

- (.Vx 50000)1 100 ml sample water r~e l -4Ukred- -- ml ml B:-Ns 500 - 4.5 S.U. number number (E) (acceptable range --0.01.-OI0 Laboratory control standard: Reference True value Sample Alkalinity (MV) % RS - Mv I TV x 100 standard number (TV) volume Begin End Total Niultiplier (mg CaCOyL) (acceptable range (Mg C2COmL) (ml) ml ml ml _ 90 to 110%) IM5sa s 100 100 0.0 o

10. 0 o /o. O9 I/O Duplicate sam le precision:

Sample Alkalinity %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOA) [(S - D) Jl(S+D)1211 x 100 number (ml) ml ml ml (acceptable ranve - 10%) OSa-M.z 5ek /M, 3 /° O . 0 S.; 5.a 6 5 5 4, Duplicate 7 s .a0.3 s 1 / I D a M atrix spike recovi y Reference Spike value Sample Spike Alkalinity (A) standard number (SV-) lvolume lBegin lEnd lTotal lMAultiplier l(mg CaCOVL) (mg C2COVL) l(ml) l-ml lml lmll JA f 8 /06 15a2.5 7 ° 3 /o '9 /1, I rSample akalinity (B) Mleasured spike value (NIV) l R MIV I SV x 100 (mg CaCOA1L) IV - A - B (acceptable range (mg CaCO/tL) = 75 to 125%) l50 50 /OD i Sample measurements: Sample volume Begin End Total AlkalinIty Sample number Sample ID (ml) ml ml ml Mlultiplier (mg CaCOJL) log38alqozm C. Im:C / 100

S.3 31.1 S.S q1 s 7 IDSO'

3ZD3I r Aopowl

^°e 3-600 s 1i. -.1 8 50

3.

5.o l.a0 R by: DaIe reviewed: 4D 9 9 I I~~w D1 I ~~ .................. -1 1 3 01 Reviewed by: LI I1 Date reviewed: I ~- 02 -S -1

'-'v': : I.. I 1 I Page Page I of. Environmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL 1.0 mg CaCO3/L Analyst Date analyzed [ 1j43O ] strant normality and multiolier determination: Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml - 0.2OE (N x 50000)150 ml sample number number (E) (acceptable range - 0.0 1S

• 0.022)

=N x 1000 INA10Z )pjq 9a3 D* O

• 1 0 L7

0. 02C c 20A0 Laboratory control standard:

Reference True value Sample Hardness (NLIV) % RS - MV/TVx 100 standard number cV) volume Begin End Total Multiplier (mg CaCO 13L) (acceptable range (mg C*CO/L) (ml) ml ml ml - 90 to 110%) 40 S.q O (, 9 -S), Duplicate sam ple precision:

• Sample Hardness

%RPD = Sample Sample ID volume Begin End Total Multiplier (mg CaCO/L) ((S -D) Il(S+DY21 x 100 number (ml) ml ml ml (acceptable range - 10%) 0-2i %-o- _Ssw (tt

2. t 7-0,(o_

S y 7 4n l Duplicate 13-f Z. I .w D 0 l Matrix spike recovery: Reference Spike value Sample Spike hardness (A) standard number (SV) volume lBeginl End Total Multiplier (mg CaCO/IL)

• (mg C&COA)

(ml) ml ml ml A3 C 9(4-0 b Jji,Ji i 4'o zo.t.-o Sample hardness (B) Measured spike value (MV) % R-MVISVX 100 (mg CaCO,/L) MV - A - B I (acceptable range (mtg CaCO/L) -75 to 125%) i£43 i C* ?.5 9, Sample measurements:. Sample volume Begin End Total Hardness Sample number Sample ID (ml ml ml ml Multiplier (mg CaCO/t) lIJ (hould be - 0 mcn CaCO,/L) T 0 l Do Z°_ _ _r D04 '15-S A r3, -142cf r I ZJ*1 21S-4 ___-4 l 0? SO.? t_( OE ZO A 1-sq D.? 1 r 05b6?I't1'5 L 2 ___ 1__ _ 20.0 3'L) 04 8.Z Note: If >l50 of titrant 'S Us Supple must be diluted. Reviewed by: I A l Date reviewed CZ0-O 1. l w

Page

9 Page 2

of..

Environmental Testing Solutions, Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst 1 Date analyzed Titrant normality and multiplier determination: f

oro-aty1-b EI

-Regin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml m I (' x 50000)! 50 ml sample number number = E) (acceptabie rnge -0.018. -1 h' 1000 Laboratory' control standard: Reference True value Sample Hardness (MV)

• . RS MV TV s 100 standard number (TV) volume Begin End Total MIultiplIer (mg CaCO*JL)

(acceptable range (mg CaCO/L) (m ml ml ml _ 90 to 110%) 1 I5 40 50 q27.s 2 .I ZO.(v 3 1 o'59 Duplicate sam ple precision: Sample T Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO,/L) ((S - D) IL(S+DY211 s 100 number (ml) ml ml ml (acceptable range - 10%) Cb 1't A09 !O.J 3 _~ - 5 20 S B. Duplicate __D5o

22.

Z 3 D 2 z. Matrix spike recovery: Reference Spike value Sample l Spike hardness (A) standard number (SM volume Begin End Total l hultiplier (mg CaCO31)} (mg CaCO3/L) (ml) tn ml ml fi~s2 65l l SD: l4 5L) 'R 21 26-3 1 tl20-to lq4 Sample hardness (B) Measured spike value (V)l % R NIV I SV x 100 (mg CaCO/L) MV - A - B I (acceptable range I (mg CaCOAL) 75 to 125%) lIb.I 102 ?. Sample measurements: Sample volume Begin End Total Hardness Sample number -Sample ED (ml) ml ml m2l Multiplier (mg CaCOA) l l ~~~(should be mg CaCOytL)l lOt:5o0 12.0-t mm M CL P Il2S.3 2(05 l .Z. l D. (a ? OSb-3aI4.o5, I Z t Z.5 Z?.7 1 I7 l Z 050o41S.02-3 Z--? 2 4 1,5Zl. l l6 I0~l > la ltn1 I I I 1 41qZl2 l l l Y Q ° I 1 33 I ltSb3ato.ICo 11 a.q 1 I  4  4-'- 4-* I I 1-1- i 1, I I I I I I  I - -7 4-1 JI Note: if > I Smi of titanlt is used, sample must be diluted. Reviewed I by: I a Date reviewedI Oa O2_cOs 1

I. I.- Page 3 O Page / of 42 Environmental Testing Solutions. Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL = 1.0 mg CaCOQL Analyst Date analyzed lL i.i -s I.5 T&iant normality and multiplier determination: Titrant Normality check Begin End Total Normality (A) of EDTA pH Factor or Multiplier reference standard ml ml ml -0.2/E (NVx 50000)l 50 ml sample number I number I (E) (acceptable range -0.018 -0.022) iVs 1000 R 16? 1,A g (A.3 0.o /o.o /0- o

0. o L0 Laboarawy control standard:-

Reference True value Sample Hardness (MV) % RS - MV I TV x 100 standard number (TV) volume Begin End Total Multiplier (mg CICO,/L) (acceptable range (mg CACOL) (mD ml ml ml .90to.110%) siss a-i 40 50 10J 0 J J 2-1 c2D 4a 105 Duplicate sam ple precision: Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg C&COIL) {(S - D) 1(S+DY0111 % 100 number (ml) ml ml ml (acceptable range - 10%) D5upliae 41 ° s iO L 4.1 Duplicate [ 0.1~ 0 o D s7

• Matrix spike recoveru Reference Spike value Sample I Spike hardness (A) standard number (SV) volume Begin End Total Multiplier (mg CaCO/L)

(mg CaCOAL) (MD ml ml I ml 10t5 aa-7 40 30 16.a C1,.31 6-/ I IQ 0 /9,2-Sample hardness (B) Measured spike value (MV) % R - MV I SV s 100 (mg CsCO3tL) MV - A - B (acceptable range I I (mg CaCO3/L) 75 to 125%) oaI40 1 lOb e/o Sample measurements:. Sample volume Begin End Total Hardness Sample number Sample ID (mD ml ml ml _Multiplier (mgCaCOA) l7_(shouldbe_-_mC_ CO_ L)l 50 0.0 0.0 0.0 °o. N° va.o5 5o .2; .3 4., aO ? I _I_ CR. m R 0 l - 1 3 (e q l 0 05S ti2. 01 l6~-1Wv 1" 1 ,sj 0.613-5.412.2 1S L, l aS23.-CA 41

G 3

1 5° O 35.0 37.3

1. 1I 5

41 ID 1 50 237.31*04l R &S UIZOO-3 0! N 1 1-5° 1 0.°

T4J T IT 1

IYSC12. O -.1;) 2 3 50 0.o-2 a. 1 s 56 5wr 60 a - 1 1 7o Note: If >1Sml of titrant is used, sample must be diluted. Reviewed [ T f l Datereviewed r by: I a I0 08-oz-s

I I I Page 3 Page - of Z Environmental Testing Solutions. Inc. Total Hardness (EPA Method 130.2) Matrix: Water, MDL =1.0 mg CaCOA Analyst [I rQ I Date analyzed OII aI.o5 Titrant normality and multiplier determination: Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml I 0.21E (N x 50000Y 50 ml sample number I numb -_er (E) (acceptable range - 0.018 - 0.022) -N I 1000 Laboratory control standard: Reference True value Sample Hardness (NMV) % RS = MIV /IV x 100 standard number (TV) volume Begin End Total Multiplier (mg CaCO3L) (acceptable range (mg C.COJ/L) (mD ml ml ml _ 90to 110%) /W1S aS Z7a7 40 50 94.o ao 4 Du licate sam lc precision: Sample Hardness %RPD - Sample Sample ID volume Begin End Total Multiplier (mg CaCOj/L) J(S - D) /[(S+Dy211 a 100 number (ml) ml ml ml (acceptable range - 106%) C051-z.tt 50N 6f 0

8) -7 6.

Duplicate I 111.5 l 141 3 D 4 i.3 A 'I/ O M atrix spike recov e y__ Reference Spike value Sample Spike hardness (A) standard number (SV) volume mlegn d Total lultiplier (mg CaCO/L) (mg CaCOOL) I(ml I[ j ml IMSsa r7 40 I so -2 0 1 Io i Sample ardness(B) lMealsuredspike value (MV) l %R -MIV/SVX 100 (mgC4COA; I - A -B (acCeptable range ( I lmg CaCO/IL) - 75 to 125%) O6 46 0 &/a Samole measurements: Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOL) l l ~~Blank I l l ~~~~~(silo ld k - 0e~yC9L otjql'2-z t C. A mr. 1-I I 7 II IoSbt.o31Q X¢ 5 Q .( /.7 ___Szg r 50G 7 _ o4 (' 1 4i3 4*O AR l. l r. 7.;a-Ro5l M qs BH.O A't 5o 124-) 12S. 4-'1 11 7.I. 15 1Hfl 1 1 16 _ I I Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: l W I Date reviewed IO--Os I

E  ,..,-. 

- w 0*-"..

.4. 2"- 4 a. S.. aA1 S., - I - 4' -..3 2. rnvirnnmental Testing-Solutioos, Inc. Page 43 Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter Accurnet Model AR25 pH/Ion Meter Analyst &P I Date analyzed Iodide reagent: I ItJ I (.Oq 1 Acid reagent: IIN R I S 15 I Calibration: .o .1. -. l 0.10mz/L 1.00 mg I Reference standard number l SS wsSa76 I Nhote: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratory control standard: Reference standard True value MTV) Measured value (MV) %RS-MV/TVx 100 number I (MtfL) (mg/L) (aceeptable range -90 to llO.) I ssy 0 .50 o.4 5-Oil, Li Duplicate sample precision: Sample Sample ED Sample charncterlstlcs Residual chlorine %RPD - {(S - D) /I(S+D)Yl) x ID00 number (mL) (acceptable range - t 10%)

mtu ioi fOcP

, CLQOe.- a S Zonoeyoe ______s_0_____ .Duplicate D zos3 j Sample measurements: uSample Sample ID Sample characteristics Residual chlorine number __(gL .Blank (shouldbe< 0.1omg/L) 4--I cSt.aoz us - 5 itJm o.t C1lQ.t (SnhJis A~o.oxo(z

u _

_, '00062'? ,.{X .k _j4_q _0D

1 rs Zr Note: All samples were analyzed in cx:es

~. Labortory control standard: 2;rt Reference standard I _ number_ S -- b~at i: s of EPA recommended holding time (15 minutes) unless otherwise noted. -a True value2(TV) Measured value (%V) l % RS - IV /TV x 100 (mg/L) (mg]L) (acceptable ranec - 90 to 110%) 0.50 0.501

0 2.

I1 Reviewed by l A Date reviewed rn

t. W.5_ Environmental Testinte Solutions. Inc. Page _ Page I of I Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mglL Meter: Accutnet Model AR2S pH/Ion Meter Iodide reagent: l7 7? Acid reagent: f//A/ le! l i Analyst I 4 v ( Date analyzed 0 2 1*S I I Calibration: I :-: v 0.10 meiL 1.00 me/L Reference standard number I/1 t9:7 r 2 c 1 Note: For samples with a residual chlorine of > 1.0 mgfL, the calibration range must be adjusted to bracket the chlorine levels of the samples. Laboratorv control standard: Reference standard True value CM Measured value (MV) % RS M .UV / TV x 100 number (mfgL) (mgaL) (acceptable range - 90 to 110%) A l i '5 ~0.50 O. 3-0 Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD - {(S - D) /I(S+D)12l) x 100 number _mg/L) (acceptable range

• 10%)

-Duplicate .1< s D Sample measurements: Sample Sample ID Sample characteristics Residual chlorine number (mg/L) Blank (shouldbc 0 0 m trmzo.oul -rVA

• ti -

t fr Oe adO. / Note: All samples were analyzed in cessot0EPA recommended holding timc (15 minutes) unless otherwise noted. Laboratory control standard: Reference standard True value (TV) Measured value (NMV) % RS -,IV/ TV x 100 number (mgL) (mg/L) (acceptable range - 90 to 110%) 5z O 0.50

0.

° oo.7 Reviewed by I Date reviewed I n'z;- 3

• .:Environmental Testing Solutions, Inc.

[Po = 4 q. Page 74 C Page I of l_ Total Residual Chlorine (EPA Method 330.5) Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst Date analyzed k1bA I lodide reagent: I /1.JR 176 Acid reagent: I /mg 16 07.a13.OS Calibration: I l O.0tn mL l 1.00 m7L l l Reference standard number I lAssa I nu sa I Note: Forsamples with a residual chlorine of > 1.0 mglL, the calibration range rmust be adjus~ed to bracket the chlorine levels of the samples. Laboratory control standard: Reference staudard True value (TV) Measured value (MV) % RS - IMlVITV x 100 number (mgrL) (mg/L) (acceptable range - 90 to 110%) 0.50 0,504 lOl O el Duplicate sample precision: Sample Sample ID Sample characteristics Residual chlorine %RPD ((S - D) I!(S+D)/21) x 100 number ( (acceptable range - s 10%) )Q42-.0) ru-R ArS&N- &0 i Au °p"41,1ts S 0, 00 o7

Al-,'8 s

-Duplicate D o.OoIg Samplemeasueaets: Sample Sample ID Sample characteristics Residual chlorine number (mglL) 7 Blank (shouldbe <0.10 mgl

f.

O. 00O4*3 C Z. 'TU-i Q aIT " l a4 ) e., &,4l' & I(

0. 00o M

?2 Q. 7.a C jtnj~CLn S o e iO dr 'c, n J Jo O. ooo > 6!5b-2S-02 Mt. 0l~ 1 98h P. JaA Co1 no 0 $4110 /f1e ffme_ Zo. 000a to OSb2-SG u e f ng o,1 06 a.4,' e ffi 5 O. 00 316 Ntote: All samples were analyzed in excess ofrEPA recomntendcd holding time (15 minutes) unless otherwise noted. Measured value (MV) (m&(L)I % RS -NIV /ITV s 100 ceptable range - 90 to I 10%) I O (. R /0 Reviewed by Date reviewed r i-a-o

Sequoyah Nuclear Plant Biomonitoring July 19-26, 2005 Appendix D Reference Toxicant Test and Control Chart

Environmental Testing Solutions, Inc. Pimephales promelas Potassium Chloride Chronic Reference Toxicant Control Chart using Moderately Hard Synthetic Water 0.9 I I I i I I A I I I I I I I* I USEPA Control Limits (I 2 Standard Deviations) 0.8 F 0.7 0.6 0.5 1.2 I O I I I I I I I I 1 I I I I I I, I I I I I I I I I I I i I I I I i I I USEPA Warning and Control Limits (751h and 9 0jh Percentile Cls) l O&O t N-
V
U O* C4 10 t-
0.8 _---...

• 0.6 no.

V.W 0.8 0.7 0.6 0.5 Laboratory Warning and Control Limits (leh and 25'h Percentile CVs) ,........................................ I..................................................... I IN AI Test date

---

-ay IC2. = 25% inhibition concentration. Am estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC.5) --Warning Limits (mean IC.5 i SA 1o or SA.75)

• Control Limits (mean IC2.s + SA _25X SA.90, or 2 Standard Deviations)

Environmental Testing Solutions, Inc. Potassium Chloride Chronic Reference Toxicant Control Chart ror Pimephalespromelas using Moderately Hard Synthetic Water Tntnum ber Tea date edaylqC Cr (DLl MD (Vl XCI) 1 11-30-04 2 12-07.04 3 01-11-OS 4 02-4S-05 5 02-12-05 6 03-01-05 7 03-01-05 8 03-0S-05 9 03-15-05 10 03-29-05 11 04-12-05 12 04-26-05 13 04-26-05 14 05-03-0S 15 05-10-0S 16 05-17-05 17 06-07-05 18 06-22-05 19 07-12-05 20 07-19-05 0.54 0.64 0.63 0.62 0.62 0.61 0.61 0.69 0.62 0.67 0.61 0.52 0.58 0.61 0.58 0.50 0.62 0.61 033 0.62 0.59 0.60 0.61 0.6t 0.61 0.61 0.62 0.62 0.62 0.62 0.61 0.61 0.61 0.61 0.60 0.60 0.60 0.60 0.60 State nd USEPA LAbartery laberatery S Central lUmit SA. Wanfn Lbntt SUs Centra Ltmltb CT4-2S CT+2S CT-S9AI CT+BAI. CTr-ASA CT+S.Ug 0.07 0.45 0.73 0.07 032 0.66 0.12 0.47 0.71 0.05 0.50 0.71 0.07 033 0.67 0.13 0.48 0.73 0.04 0.52 0.69 0.07 0.53 0.68 0.13 0.48 0.73 0.04 0.53 0.69 0.07 0.54 0.63 0.13 0.43 0.74 0.03 0.54 0.68 0.07 0.54 0.63 0.13 0.48 0.74 0.03 0.55 0.67 0.07 0.54 0.68 0.13 0.4t 0.74 0.04 034. 0.70 0.07 0.54 0.69 0.13 0.49 0.75 0.04 034 0.70 0.07 0.54 0.69 0.13 0.49 0.75 0.04 0.55 0.70 0.07 055 0.70 0.13 0.49 0.75 0.04 035 0.70 0.07 055 0.70 0.13 0.49 0.75 0.05 0.52 0.71 0.07 054 0.69 0.13 0.49 0.74 0.04 032 0.70 0.07 054 0.69 0.13 0.48 0.74 0.04 033 0.70 0.07 0.54 0.69 0.13 0.48 0.74 0.04 053 0.69 0.07 0.54 0.68 0.13 0.48 0.74 Q05 3 031 0.70 0.07 033 0.68 0.13 0.48 0.73 0.05 Om 0.70 0.07 0.53 0.6S 0.13 0.48 0.73 0.05 051 0.70 0.07 0.53 0.68 0.13 0.48 0.73 0.05 031 0.70 0.07 0.53 0.67 0.13 0.47 0.73 0.05 031 0.69 0.07 053 0.67 0.13 0.4S 0.73 USEPA SA1 WarnIng Limrts CT - SMA CT + SAYS USEPA SA. Confrol imits CT -SAWe CT + Sx, 0.22 0.37 0.31 0.27 0.23 0.37 0.83 0.27 0.23 0.33 0.84 0.27 0.23 0.38 0.84 0.27 0.23 0.33 0.84 0.27 0.23 0.38 0.84 0.27 0.24 0.33 0.35 0.28 0.24 0.38 0.85 0.28 0.24 039 .86 0.28 0.24 039 0.56 0.28 0.23 0.38 0.85 0.28 0.23 0.38 0.84 0.28 0.23 038 0.84 0.28 0.23 0.38 0.84 0.27 0.23 0.37 0.83 0.27 0.23 0.37 0.83 0.27 0.23 037 0.83 0.27 0.23 037 0.83 0.27 0.23 0.37 0.83 0.27 0.32 0.86 0.12 0.33 0.87 0.09 033 0.Q8 0.07 0.33 0.88 0.06 034 0.88 0.06 033 0.88 0.05 0.34 090 0.07 0.34 0.90 0.06 0.34 0.90 0.06 0.34 0.90 0.06 0.34 0.89 0.07 0.34 0.89 0.07 0.34 0.89 0.07 0.34 0.38 0.07 0.33 0.87 0.08 033 0.88 0.08 033 0.88 0.08 033 0.37 0.08 033 0.87 0.08 CV Note: 7id IC,, day25% Mi'hJ emnaenrtim An eastini ofthc coteenftitOn ofpotaim Chloride that would cane a 25% reductin in Pimephals growth for the test popUitbLn CT - Central tenadency (mean IC,,). S - Standard devio of the MiC. vkeas. Laberatery Central and Wanint Limts LAbonteacostrolnd wa2ring lnwere tablinhedusigthe rblldard devi ofthe IC svaes conapondngto the 1th and 25th percnt CVs. Thes rangs ar more stribnt thta the contol and waninglinks recommended by USEPA for the teat method and endpoint SA - Standard detion caneApodinc to the 10' peretile CV. (SA - 0.12) Sus - Standard devitiba correspondkkg to the 25' oPercentie CV. (SA1 - 0.21) USEPA Centrol and Wazidn Lhntba SA.n5 - Standard deviatim codrespoading to the 75' percentile CV. ( S,, - 0.38) SA"- Sbndard delobn comiponding to tbe 90' perecntile CV. (St -0.45) CV = Coefficient of aiation of the XC2, values. USIIPA. 200QW4 nd thSriMtt o bi iy tStlbenticlypptutb dstatath arobhtatkP im. Ets-. llU-xxninmtaAenchCc.tL Orlt.M.he@tafroZw at.:rl. I 07 1w4

Environmental Testing Solutions, Inc. Pimephalespromelas Control Growth, Coefficient of Variation, and PMSD in Potassium Chloride Chronic Reference Toxicant Tests 1.25 1.00 C5 0.75 .6.E 0.50 v i0.25 30 o v 20 O~ co (10 o 0 40 I - I I f 2.... per s n a USEPA Acceptance Criteria (> 0.25 mg per surviving larvae) I I I T l B I B r r I r I I B I I I Kentucky Acceptance Limit (< 30.0%) 30 1 USEPA Upper PMSD Bound (90e percentile < 35.0%) I.I ..I. I.I..I.I.I..I.I.I..I.I.I..I.I.I..I.I.. 20 1 10 aI 3v-t1 a 0k. 'VOJb pA5 1 Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Growth, CV, or PMSD) Control Limits (mean Control Growth, CV, or PMSD k 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean CTv CT MSD PMSD CT Survival Growth for Control Growth (%/) GrowthrCV (%)( ) forPMSD () (gare) (mgllarvae) GorCowth o (.V) o(PSD(% 1 11-30-04 100 0.689 15.7 0.10 15.1 2 12-07-04 97.5 0.687 0.688 7.6 11.7 0.09 12.7 13.9 3 01-11-05 100 0.797 0.724 8.7 10.7 0.10 13.0 13.6 4 02-08-05 97.5 0.687 0.715 3.9 9.0 0.11 15.5 14.1 5 02-12-05 100 0.712 0.714 5.8 8.3 0.11 16.0 14.4 6 03-01-05 100 0.749 0.720 11.8 8.9 0.17 22.7 15.8 7 03-01-05 100 0.889 0.744 4.5 8.3 0.12 13.7 15.5 8 03-08-05 100 0.735 0.743 8.3 8.3 0.08 11.0 15.0 9 03-15-05 100 0.533 0.720 3.1 7.7 0.06 10.5 14.5 10 03-29-05 100 0.676 0.715 12.5 8.2 0.12 17.4 14.8 11 04-12-05 100 0.702 0.714 9.8 8.3 0.10 13.9 14.7 12 04-26-05 100 0.742 0.716 3.9 8.0 0.09 12.3 14.5 13 04-26-05 97.5 0.702 0.715 7.2 7.9 0.10 13.5 14.4 14 05-03-05 100 0.760 0.718 2.5 7.5 0.10 12.9 14.3 15 05-10-05 100 0.772 0.722 10.7 7.7 0.10 13.5 14.3 16 05-17-05 100 0.858 0.731 6.0 7.6 0.06 7.4 13.8 17

• 06-07-05 100 0.848 0.737 3.3 7.4 0.09 10.1 13.6 18 06-21-05 97.5 0.622 0.731 7.2 7.4 0.09 14.5 13.7 19 07-12-05 100 0.765 0.733 5.6 7.3 0.10 13.4 13.6 20 07-19-05 100 0.596 0.726 2.9 7.0 0.06 9.7 13.4 Note:

CV-Coefficient of variation for control growth. On average, the CV for control growth is 7.0% in Environmental Testing Solutions, Inc. Pimeph.es chronic toxicity tests. Lower CV bound determined by USEPA (10e percentile) - 3.5%. Upper CV bound deternined by USEPA (90' percentile) - 20ff MSD - Minimum Significant Difference PMSD - Percent Minimum Signifcant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test On average, a significant difference occurs for Envirommental Testing Solutions, Inc. cronic toxicity tests when a toxicant reduces Pinephales growthby 13A% from the controL Lower PMSD bound determined by USEPA (10' percentile) - 9.4%. Upper PMSD bound determined by USEPA (90' percentile) - 35%. CT - Central Tendancy (mean Control Growth, CV, or PMSD) The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 labontories for Pimephales growth in ehronic reference toxicant tests. USEPA 2000. Undertanding and Accounting for Method VriabiLty inWhole Effluent Toxicity Applications Under the National Pollutant Dischrge EEmimtion Programn EPA-833-R-00-03. US Envirmnental Protection Agency, Cincizmati, OR Organisms obtainedfrom Aquatc BfoSystems. In0 07 19-05

<-<:Envonrental Testing Solutions, Inc. Potassium Chloride Chronic Referen (EPA-821-R-02-013 Method Species: Pimephalespron 1.. .,.F. :. .-Pa. lo .f, S. I C. Page I of9 S 'ce Toxicant Test 1000.0) melas .PKCICR Test Number. 1 0 I.. I Dilution preparation info mation: Comments: KCl CHM number WM te Stock preparation: 50 g KC1/-L Dissolve 50 g KCI in l-L Deionized water Dilution prep (mg/L) 300 450 600 750 900 Stock volunw(fl) .6 9 12 is IS Diluent volunw(mL) 994 .991 98S 9S5 982. Total volune (niL)0 1000 1.00 1000 Test organtsm information: T Test information: Organism age: zz.S -rM wt~ogS

t.

Randomizing template: Date and times organisms i*Os 0 11;

T tSOC Incubator number and were born between:

shelf location: 3 Organism source: PTBS XE (SFIFOS Arsemia lot number Transfer bowl information: pH = SU. Temperature 'C Total drying time: 2 .3 Date /Time in: ava oS 131 0. Average transfer volume: Date / Time out: tn-115-6 3 Oventemperature: 7.. .V.I. Dailyfeeding and renewal information: Day Date Morning Afternoon Test Initdation, Ms Analyst'. fceding feedingtUme renewal, or batch used time termination time 0 0 1 1°-CS 1530 l 3Z. 2 oizic 2leSC~s3 I~~l l32-.. 61-i3-o5R lei_ O13 G-A 3 0S ffROS %Soo %15 O >3.. 4 Cl 23-as CRtOO sq131 c 3o S C_-24_ CS WA1ZI I IZS5 oi3.oSA. L. 6 m*2s-aS 08 12440 A 7 al

• L*OS g:21301

\\ Control information: Acceptance criteria Summary of test endpoints: Morali: s 20% s_ 7-day LC50 1L1. 6e Average weight per initial larvae: _ p. 0q ( NOEC 3OC Average weight per surviving larvae: 2 0.25 mg/larvae LOEC 300 IChv 1
-14ev) _h _. b62.5

Environmental Testing Solutions, Inc. X..;Z..

• P age:aS b.r Species: Pimephalespromelas PpKCICR Test Numl Survival and Growth Data Day Control 00 m KCIL m KCIIL

_____A B IC D 1E IF G H I J K L 0/ 1o 10 /0 10 10 I0 to10 I _ l D ID I 10 1 I- -10 10 .2 It O { 0 1 C O 1 O 1 I C) l 3 1 1/0t10o10 to / 100 to IC) 3.to 4l to J /0 (010 Io0 (0 o 10 10 1/ 4._ 10 iO 10 t O0 t 10 10 10 6t _ o 1 iC lC) it) to 1 to I 10 6 ID tO 110 to el I0 0 1 0 ~ 0 tO IL) A - /_. 10 _0 to 0 /0o to A Pan weight (mg) RAM 'Trax color eqd: 152. K1s.7 I 6Z 3 1 I. q ° iq.sqt 15.-tS K'l82-15e19 ISJ Z 1(..! yji.q2. .1..q Analyst:i_ B - Pan + Larvae weight (Analyst: a1J-. L.. al.'S aa.02,i.ao [i7 a,*7 1141 0f l ao ao D Larvae weight (mg) - A - B 5tq tb S. 1q. 535lZ;.os S~os qjsn !slt le.A

5.it Weight per Initial number of larvae (mg)

-C I Initial number of larvae 0@ "Oil, C2 I0* Average Percent weight per reduction Initial from control 0 5 1 number of (%) I rvac (mg) I - S - I - a - .i.4.. ' 0 *SZ1 6 J. a-I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig unusually large, d&r = decanted and returned, w = wounded. Calculations and data reviewed: Comments:

Environmental Testing Solutions, Inc. '., of Page 3 of' 5.^ Species: Pimephalespromelas PpKCICR Test Number: - O Survival and Growth Data Day 600 mg KCI/L 750 mg KCIIL 00 KCVL _M N O P.2 R S T U V WIX O. ID IO /10 6 1o1 001 1 J I0 I0 1 0 i_ 10 .i 41-A Fi H sl 3 3 2 I_ 10 1 9 o 5 3 3 3 4- -o ` 5 3 20 5 so1 1 g 9 4 6. 4"4 z'4 6 L, ? 7 Ir LAW~ _O Ia &f 6 '2_ A - Pan weight (mg) _ Lb Tray color code:: P Iq.13 16.1 .24 tL2 i. 15f t01 15.O j s5.1i i.K 1 qA l

4. 3 tq.L A:alst1:~z B - Pan + Larvae welght Analyst:

ilk o ID. S I5Yf i4J Larvae weight (mg) - A - B qA4 A4. 4.17. S*ot s.01 3.3>1 14.I'i '3.5% 0.qq 1.10 o.-s Weight per Initial number arlarve (mng) h \\O dy -C J Initial number of larvae O O O O O v O Average Percent weight per redaction Initial roenctontr o.4lb3Z65 a s' 43

o. 32.

o. 06 c 37.

number of (%) larvae (me) Comment codes: c = clear, d = dead, fg = fungus, k killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded. Calculations and data reviewed:4 Comments:

Environmental Testing Solutions, Inc. Chronic Whole Emuent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Plmephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test maban PpKCICR I I st(a79 at 351 Depot St.) Teat dateel Juy mid, 200 Reveiwod %Y/ '~ C-as 3.4. aq.V* Ialedalaoo l 3r r".b A

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0.444 1330 0A3 931 22.3 e 3 0 e 35.24 19.36 4.32 0.3n 01432 30 9 16.17 23.I 5301 03 55 0.50 .O.S Q 10 6 IS 95 1902 3.07 0.532 0.301 75t It 10 16 01 19.33 3.37 0.421 0.492 9.7 0.337 7 n.s 6J5SS 1a3 40oa 10 a 15.23 13947 4.19 0.524 04139 T 30 I IS.M 1169 3.38 0333 0.3335_ U t 4.46 149 049 0.490 0049 V t0 2 3 .9 O. 030 0 430 36.9 0.110 1,. 0.U41 12 MI W 30 I 1453 14.7 0.25 O 0025 X 30o 0 000 000_ 0 00 0000 0000 I Dtt'1 MSD VAct I'MSDI 0.037S 9.7 MSD - Mfbib Stpignuct Difiome-e 33.SD - Faver4 Minimro SipU~kont Difference 9PeS.4D sawe sa n of tt i p ciion The tMS Dtheminihmm p rentdiffenC etweew the trl d thgt esabe decleds wsticfyll rticusg h osol Junttoxcitt. Ot aSr; dm slrerns a Troand Sonerotosb y1 dhonic toticxotyt at r es im l g by .4% m the controt (detmubed ftho h ftutu touiant tentl6g} LowtPMSD bound etmined byUSEPA (ItO pnule) - 9.4% Upper MSD bosad detrmned byUSEPA (90th pecntle).-33% Thelowerandu bpperbun we eicultebytheUSEPA uings20 tests D od educoot 19oaeo rods for imephale gowtin rna re nceKtoxkm te. USESA. 2000. Udrmnding wi Atootetio for Method VarfsIai ny n Whote eent Touccy A~pflcdn nderthe National Polutol DVcltrhare Elimination Propun. EPA-833-lt0 US EnvimentA P3otecon Agency.CnsdscmIl. Oxezarwt obeelanedfrow Avetk SoSyoInt. Inc. 07.19-05

Environmental Testing Solutions, Inc. Statistical Analyses ,, _LM Fish GrCb sad SorvTesit.7 Day Sarival SaxtDat: 7WM=0 Test ID. PpKCR Sample 1DP REF.RxfTdxkanl End Dtet 7/2n62co Lab ID ES.m k Tatt SO1 Sa-ple pc KCLPoubm j el Sample Data: rotocol IWCHRI.PAW

2.

2.R2013 Test Spect : PP.?imephilm Pecehs Comments: Caea-mflL I 1 5 4 D-Cool 1.0000 IJO lOXDO 1.0000 300 Oxw~ 1.0000 L0000 LWOOD 450 O.0 L.o l 1.OX l.00X 600 1.000 0.7000 0o10 0.9000 750 .600 0.1000 QIC O 0.7000 900 0.2000 000 0.2000 0.00o Trufar= Aruh Square R.st Rank I-T" Number ToW CaeumzlL Mesa N.Mesa mIe s MLb Mle CV% N Sam Cided Rasp Nomber DOCoolr 1.0000 1.OXO 2.4120 2.4120 1.4120 0.00O 4 0 40 300 0.9750 0.9750 13713 1.2490 1.4120 3.42 4 16.00 20.00 1 40 450 o9750 o.97 1713723 2490 1.1220 1942 4 2600 20.00 I 40 600 01500 0a1500 1.229 099l2 2.4120 11.51t 4 22.00 20.0o 6 40 -70 0.7250 0.7250 1.0229 0.1161 11071 10W36 4 10.00 2oD0 12 40

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20.00 10.00 36 40 AuelliuTlats Stadafe Oleical Skew Kart ShapomW&s Test ndicate nmldotutiso (p> 0.0) 0.94493651 0.04 4132957 0311192149 Etuabry of vne cuonnt be ecwfimed Ityedeals Tat (1.tll. 6.99) INOWC LOilC OV TU Steals lis4ny 6 Rank Test 600 750 670.8203932 Trnenta u D Coatlol Maximem Lk&Dlba4r.bleb Pramter Vale* St ffFUYldseWLbu COMl 012.Sq Citile rPNaub. Me SiRs liar Slop. I.103164432 5.754692465 *10.210135 26.41716324 0 63.8623447 7.11472713 1.2-14 281479048 0.12340578 5 ltercept .11.3759316 16.a30L09.70.663551 333S169411 SCR 1.0 Palx: Prultbi c gL 95% FPael t imt /e Ea=t 2674 396.0016561 EC05 3355 410.6195412 0.1 ECIO 3.712 532.3211041 0.7 EC15 3.964 S71327273. 2020 4.152 603.1474035 0.6 EC25 4326 633D71361 01 E0WM 4.747 713.7157421 I EC50 .000 766.991371 0.4 EC60 5.253 224.2A43091 03 Ec75 5.674 929.069252 0 EC3 O.142 97421264710 E15-6.036 I20.663367 0.1 EC90 6.212 IIC3391t6 0.0 EM95 6.645 1225394636 I 100 1000 10000 EC99 7326 1415531305 t nt gtO SgaUSOM hUlsgeele y deWCstd (p - t7 ne.24) Dae-Respea nda Orgnodou vbkaedfivm ApaewttBioSystatme lao. 71.O 07-I94

Environmental Testing Solutions, Inc. Statistical Analyses larva Fil Crrh ad Sertival 7 t.7 DsYCr"wU Sent Date: 719/2005 st ID. pPK= 3amplI R1EP.RdTaxiut 2ndData VW22005 Lab M bik.rstigSoI Sampk )pc KCCLPokttica Sa. ple Datec Protowt rWC4R.UEPA t21-R42413 Test Specis: PP.Pimphaks ptwuiali Cs r-.L 1 2 3 4 D.CockoI 0.50140 6030 0.5790 06160 300 0.4930 0.5120 0.5Q50 05050 450 0.4720 0.5160 0.6040 W5110 600 0.4940 0.4440 0.4120 0.5010 750 3070 0.3370 0.4190 0)510 900 0.0490 0.110Q 0.0250 OO Trhjfam: Vatbsmismad 0-Taiblld 149.sKc C"te-/L Mess ?4Me"s Meas MIX Mal CV% l t-Stat Crticl MSD Mea N-Mes D-Conl 0.5955 I CWO 0.5955 0.5790 0.6160 2U70 4 0.5955 1.09

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5.215 2290 O.575 0.4628 0.7771 750 0.3553 0596 0.3553 03070 0.4190 13335 4 0.3553 0.5964 900 0.0460 Q.0772 0.0460 0.MW 0.1100 11 3 4 0.0460 Q00772 AsxilsryTests Stsdsrc Crtical

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EnNironmental Testing Solutions. Inc. Page 4 of 5 Envi4-~~~ ~ ~ -~ronena Tetn Soui-s _nc o __ __, Species: Pimephalespromelas PpKCICR Test Number: _2f IDaih, Chemiktrv: Day .Cr 0 1 2 Analyst IF xel A57 Conctntratlon IParameter i lg CONTROL pH (S.U.) Dfl) (mzUL +.0- Y RT7-.q--1 I -f- --t-* I 1
7
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E~nvironmental Testinp, Solutions. Inc. Page 5 of S Environmental Testing Solutions. Inc. Page 5 of 5 Species: Pimephalespromelas PpKCICR Test Number. 10 4 ________Analyst N I Concentration Parameter V 4 CONTROL pH(SU) _1.69 i _7.C5 1 _7i77 DO (mglL) X-X1, O7.-I 2 a Conductivity (gmhios/cm) l Alkalianity,)wMW1ss (mg CaCOAI) Hardness (HmagrCnaeCO3 L) g2 mm Temperature 24.5 24W'i 25b 1 sA 4.3 pH (S.U.) ^6 1 .S .9 4 7498 7 v.S

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Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphnia dubia using Moderately Hard Synthetic Water 1.12 1.10 1.08 I I  I  I I I I I I I TI I I USEPA Control Limits (I 2 Standard Deviations) T_ I i 1.06 F 1.04 I-V I...... I I I I I I I I I . I I I I I I I 2.5 I V

• 4 U

0-C: 2.0 1 1.5 I 1.0 I 0.5 I 1.4 1.3 USEPA Warning and Control Limits (75th and 9 0 h Percentile CVs) Laboratory Warning and Control Limits (10 2h and 2 5 'h Percentile CVs) 1.2 1 1.1 1.01 0.9 1 0.8 o ok .V' O OS Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population. Central Tendency (mean IC25) Warning Limits (mean IC25 +/- S A10 or SA.75) . Control Limits (mean IC25 S S or 2 Standard Deviations) 25 A1_A.

Environmental Testing Solutions, Inc. Sodium Chloride Chronic Reference Toilcant Control Chart for Cerfodaphnia dubia using Moderately Hard Synthetic Water Test nunber Test date 7-day IC CT (g/LNaCI) (C/LNaCI) State and USEPA S Central lnit, SA. CT-2S CT+2S Laboratory WaNiln Llits CT* s,, CT +SA Laboratory SMS Control Units CTr SAs CT + SAM USEPA SAj Waning Liits CTr-SA.5 Cr+SA.S7 USEPA SA. Control Units CT - SAM CT + SAM CV 02-03-04 2 03403-04 3 046-04 4 05.04-04 5 0608-4 6 07-07-04 7 08-10-04 S 09-14-04 9 09.14-04 10 10-05-04 11 11-02-04 12 12-07-04 13 01-11-05 14 02-08-05 15 03-01-05 16 04-055 17 05-03-05 IS 06-07-05 19 07-12-05 20 07-12-05 1.06 1.07 1.07 1.07 1.06 1.06 1.06 1.05 1.07 1.07 1.06 1.0O 1.06 1.05 1.08 1.09 1.07 1.05 1.06 1.06 1.07 0.01 1.05 I.OS 0.09 1.07 0.01 1.06 1.08 0.09 1.07 0.00 1.06 1.08 0.09 1.07 0.01 1.06 I.OS 0.09 1.07 0.01 1.05 1.08 0.09 1.06 0.01 1.05 L.OS 0.09 1.06 0.01 1.05 1.08 0.0O 1.06 0.01 1.05 I.OS 0.09 1.06 0.01 1.05 1.08 0.09 1.06 0.01 1.05 1.08 0.09 1.07 0.01 1.05 1.08 0.09 1.06 0.01 1.05 1.08 0.09 1.06 0.01 1.04 1.08 0.09 1.06 0.01 1.04 1.08 0.09 1.07 0.01 1.04 1.09 0.09 1.07 0.01 1.04 1.09 0.09 1.07 0.01 1.04 1.09 0.09 1.07 0.01 1.04 1.09 0.09 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 O.I 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.9S 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.98 1.15 0.18 0.89 0.89 0.89 0.89 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 0.88 1.25 1.25 1.25 1.25 1.25 1.24 1.24 1.24 1.25 1.24 1.25 1.25 1.24 1.25 1.25 1.25 1.25 1.25 1.25 0.48 0.48 0.48 0.4O 0.48 0.48 0.48 0.48 0.4S 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.48 0.59 0.59 0.59 0.59 0.59 0.59 0.58 0.58 0.59 0.58 0.59 0.59 0.58 0.59 0.59 0.59 0.59 0.59 0.59 1.55 0.66 1.55 0.66 1.55 0.66 1.55 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.54 0.66 1.55 0.66 1.55 0.66 1.54 0.66 1.54 0.66 1.54 0.66 0.41 0.41 0.41 0.41 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.41 0.41 0.40 0.40 0.40 1.73 0.01 1.73 0.00 1.73 0.00 1.73 0.01 1.73 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.73 0.01 1.72 0.01 1.72 0.01 1.72 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.73 0.01 1.72 0.01 Note: 7dICis-7-day25 inUbitionconcentration. An estimsaon of lte concentration of sodiumechorlde ohat would cause a25%reducton in Certodaphnta reproductionforthe test population. CT - Central tendency (tcan Ci,). S - Standard deviation oftho ICs values. Laboratory Contyul and Wani LlUnits Labortoycontrol and waning limitswer esblishod u-ing theodandrd devation ofthe ICI n1uesaconuxponding to th 1Oth and 25thpementile CVs. Thse ruges ar nn stringent than thoecontrol and arning limita reconmended by USEPA for the test netled and endpoint SAss - Standarddeviationeorespodingto the l0 0pcreentile CV. (SA,- 0.08) S^., - Standard deviation correynnding to the 25& percentilo CV. (SA21 - 0.17) USEPA Cortrol and Wanint Lnits SAYs - Standard deviation corresonding to the 75* percentile CV. (SA.,s - OA5) SA - Standrd deviation corresponding to thG 90o percentilo CV. (SAr - 0.62) CV - Coefficient of variation of(lie IC,1 values. USEPA 2000. Undentanding and Aecoiming forMtMthd Vriability in Whol EffluentToxidcity Applications Under thoNational Pollutant Discharge ElitnntionPogram. IEPA-833-R400-003. US rnvitonmental rmtecton Agency, Cincinnti, 011. 07.1e.03

Environmental Testing Solutions, Inc. Ceriodaphnia dubia Control Reproduction, Coefficient of Variation, and PMSD in Sodium Chloride Chronic Reference Toxicant Tests 40 35 r 30 .....2s............................................................................................................... 20 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female) I I I I I I I I I I I I I I I I I I I I _I I I I I I I I I I I I I I I I I I I I s:4 = 4 ~North Carolina Acceptance Limit (< 40.0%) c! 30 S. P: Kentucky Acceptance Limit (< 30.0%) o 20:: 1 0 I.- U 4e 0 I I I I I I I I I I I I I I I I I I I I 40 I I.. L8 I I I I lI I 1 1 1 1 1 1 1 USEPA Upper PMSD Bound (90* percentile < 37.0%) 30 20 10 0*Ito rpI I I I I Tes dae Test date -.-- @Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant. Central Tendency (mean Control Reproduction, CV, or PMSD) Control Limits (mean Control Reproduction, CV, or PMSD + 2 Standard Deviations)

Environmental Testing Solutions, Inc. Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphnia dubia using Moderately Hard Synthetic Water Tes Test ate number Control Control Mean Survival Reproduction (N) (offspring/female) cr for Control Mean Reproduction (offspring/female) CV CT MSD PMSD cr for Control (%) Reproduction CV (0/.) (%) for PMSD (%/) 1 02-03-04 2 03-03-04 3 04-06-04 4 05.04-04 5 06-O-04 6 07-07-04 7 08-10-04 8 09-14-04 9 09-14-04 10 10-05-04 11 11-02-04 12 12-07-04 13 01-11-05 14 02-08-05 1S 03-01-OS 16 04-05-05 17 05-03-OS 18 06-07-05 19 07-12-05 20 07-12-05 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 30.7 29.0 28.1 32.5 32.9 33.3 27.4 28.7 28.8 29.6 30.5 31.8 31.0 30.0 29.9 32.4 29.9 30.4 30.4 31.1 29.9 29.3 30.1 30.6 31.1 30.6 30.3 30.2 30.1 30.1 30.3 30.3 30.3 30.3 30.4 30.4 30.4 30.4 30.4 7.2 7.8 6.2 6.4 6.8 6.3 5.8 4.9 7.5 8.2 8.5 4.9 9.4 6.8 6.6 6.9 7.0 5.0 7.5 7.2 2.7 7.5 3.0 7.1 2.8 6.9 3.3 6.9 3.0 6.8 2.6 6.6 2.3 6A 2.4 6.5 3.0 6.7 2.8 6.9 2.7 6.7 2.5 6.9 3.1 6.9 2.4 6.9 2.9 6.9 3.1 6.9 2.9 6.8 2.6 6.8 2.7 6.8 3.2 8.8 10.3 10.0 10.1 9.0 7.8 8.6 8.2 10.5 9.6 8.8 7.9 10.0 8.0 9.9 9.6 9.8 8.5 8.8 10.2 9.6 9.7 9.8 9.7 9.3 9.2 9.1 9.3 9.3 9.3 9.1 9.2 9.1 9.2 9.2 9.2 9.2 9.2 9.2 Note: CV - Coefficient of variation for control reproduction. On average, the CV for control reproduction is 6.8% in Environmental Testing Solutions, Inc. Ceriodaphnfa chronic toxicity tests. Lower CV bound determined by USEPA (10' percentile) - 8.9%. Upper CV bound determined by USEPA (90' percentile) - 42% MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mnnimum percent difference between the control and treatrnent that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Sohrtions, Inc. chronic toxicity tests when a toxicant reduces Cerodaphnla reproduction by 9.2% from the control LowerPMSD bound determined byUSEPA(10' percentile) - 11I%. Upper PMSD bound determined by USEPA (90' percentile) - 37%. CT - Central Tendancy (Mean Control Reproduction, CV, or PMSD) Tle lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Criodaphnia reproduction in chronic reference toxicant tests. 07-12-05new

Enironmental Testing Solutions, Inc. Page 1 of 6 Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0) Species: Ceriodaphnla dubia NePIJ AL6Ae CdIXaCLCR #: 38 Dilution preparation information: Comments: NaCI CHM numbers . H 1Cl Stock prparatiort 100 g NaCIl (dissolve SO g NaCI En 500 ml deionized water) Dilution prep (mgfl.) 600 800 1000 1200 1400 Stock volume (mL) 9 12 I 15 I 8 21 1 Diluent vohame (tL) 1491 1488 1485 1482 1479 1 Totl volume1(5L) IS00 IS00 1500 1500 1500 1 1 Test organism information: Test information: Organism age: < 24-hours old Randomizing template: JKAQ Date and times organisms CA-i-l- J5 l -t3 Ze COG Incubator number and I 7 were born between: shelf location: Organism source: a-oS-0oS A-F YCT batch: 0I _2_-aS Transfer bowl information: pH = 1,.1 SU Temperature = . CC Selenastrum batch: Daily renewal information: Day Date Test Initiation, MHS water Analyst renewal, or batch used termination time 0 a -_ ai6o s-c A t el: n os MR on-o8-. s 6 2 _-__-OS__ AT l o-o -oS C C. 01-17-6 Oa t)&o 01 - G&-ci' C_ .6 1903-05 oS 7 ir o Olt maim. Control information: Acceptance criteria Summary of test endpoints: % of Male Adults: Z ) i S20% 7-day LC50 >',IOo) % Adults having 3' Broods: 1007i a 80% NOEC EOO % Mortality: aS, s 20% LOEC 1000 1 Mean OffspringFemale: .l 15.0 offspring/female ChV .1 %CV: <40.0% IC2S I257.9

Environmental Testing Solutions, Inc. Page 2 of 6 Species: Ceriodaphnia dubia IWJeSJ AL6A& CdNaCLCR #: a CONTROL Survival and Reproduction Data RepUcate number Day 1 2 3 4 5 6 7 8 9 10 Adult mortality

l.

L l Adult mortality 3 Young produced - ) S l_il_C Adult mortality T T1 l1 4 Young produced 's ^S Li S 5 4l45l5E S Adult mortality ! C \\- :- \\. \\.- L <1 S Youngproduced Ir ,0 z I 0 I ' I T Adult mortality l l1 6 Youngproduced l 1 3 l C) Qi O L I iO Adult mortality C. U L 7 l Young produced C) 0 1 l1 -I %.5= l II C) D Total young produced -z 29 35 lZ. 30 3o l 3

32. l 3 T Final Adult Mortality*

I L L-l I l L-ll Xfor3' Broods l ll1 'X, I ^ C I Note: Adult mortality (L - live, D - dead)

• t CAf lk olete Concentration:

l %Mortality: I OMo l Mean Offspring/Female: l 3,1., 600 mp-NaCL/L Survival and Reproduction Data Replieate number Day l I l-2 3 4 5 6 7 l 9 10 1 Young produced 00 0 D 0 Adult mortality l L. L I U. -l

2.

Young produced j ) 0 0 3 lYoung produced 09t a Adult mortality U UC U UIL 4 Young produced L k 01 7 Adult mortality U

• l L

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l l Adult mortality l L C X l ll 6 Young produced l f 0 O 10 1 j l 0l I S l...LO, Adult mortality L L 5 r 67 Young produced (0

• ~

Ct) DM 0.L.. S I l Totlyoungproduced f 3 j j 0 0 31 0 1 30 Final Adult Mortality U ~ ~ W T Note: Adult mortality (L - live, D - dead) I Concentration: Mortalit: o l Mean Offspring/Female: 3 3oA % Reduction from Control: 2.(o3

Environmental Testing Solutions, Inc. Page 3 of 6 Species: Ceriodaphnia dubia tJNe AL6.4 CdNaCLCR #: I38 800 mr NaCI/L Survival and Reproduction Data = Replicate number Dar 1 2 3 4 1 6 7 a 9 10 I Yon prdue O 0 O 0 C ] Adult mortality L l L - U U t _U 2 Young produced ) C O C) I OJ _ Adult mortality L-U 3 Young produced CC). 1.

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Adult mortality l t_ l i IC1 4 Young produced 3 I I vS I1 tq 5 1 1111 1 Adult mortality I L I I S Young produced IC) 11 I O I \\ 9 I = Adult mortality J L-tC L - 6 Young produced ( D) l o lO l 1 IS _d Adult mortality j l I l Wi%--1Lt L-L- U 7 Youngproduced l 5 5 lIi l tl3 1I(D l Cl I o l Totalyoungproduced lrZ 1 -2 T 31l C 20 Z Z1 j30 I 3 Final Adult Mortality T L lL lE L lC LE - Q l l l* Note: Adult mortality (L.- live, D - dead) l Concentration: % Mortality: l T l MeanOffispring/Female: _ 2-I.L % Reduction from Control: LI.R 1a I 1000 mg NaCllL Survival and Reproduction Data Replicate number l Dy l I 2 3 4 5 6 7 8 9 10 I Young produced J Adult mortality _l l

l.

L U L. fT? 2 Young produced l) C) p l p OO Adult mortality U!.. l-U. U j ] 3 Young produced lOl O l Adult mortality TC .j L U U 'I. L I U Adult mortality L L l L T? l l1 l Young produced [ l l l F 5T Adult mortality [ g

• l LE U

l Ll l C Young prodsuced l 14 l OI I (g) l O l C 0 1 12-Adult mortality U + I t__ 0 l L-I 7 Young produced jd nI i5 i__ 3__ i_ 13i Total young produced 211 zl 2A 1 l 21 2T 2z 1 2 2 2.1 Flinal Adult Mortallty L l W W l 7 l C ll Note: Adult mortality (L - live, D - dead) I Concentration: %Mortality: l Cm I Mean Offspring/Female: I Z(- % Reduction from Control: I.870

Environmental Testing Solutions, Inc. Page 4 of 6 Species: Ceriodaphnia dubia NJWJ AL6-Ae CdNaCLCR #: 3& 1200 mng NaCIIL Survival and Reproduction Data [. Replicate number Day l 2 l 3 1 4 5 6 1 7 8 9 10 -dult-mortalit y m -L L-L- 2 Young produced l) a IQJ

c.

0 L-o Adult mortality lL I \\ i.- L L L I I 3 Youn prdced0 Cl C) 0>o11 b° I____ _ _ -Ie _L Adult mo rtalit y = 4 Young produced t l A Il 1 a I Adult mortality I \\- Young produced tA ~ I'~ LA. Lk.... Adult mortality I L-1 _lLIL l1 L 6 Young produced _I ( ) iO Adult mortality U.. LlL l l I-1-........... 76 Young produced l 6 I Total young produced IC)0 1 i 2 5 I L Z i Final Adult Mortality I l Note: Adult mortality (L - live, D - dead) l Concentratfon: % Mortality: : 63% i Mean Offspring/Female: I ltI~.2 I % Reduction from Control: 1 si2.'.4"7 1 1400 mg NaCl/L Survival and Reproduction Data [ Replicate number Day 1 2 3 4 5 6 7 8 9 t1 I Young produced l 0 C C) I --- Adult mortality ! 2 Young produced lOlOlC )lOlt )lUlOlC 2 _n_ p p m Adult mortality _t 3 Young produced l l 0 Q 0 iG Adult mortality j T~i.. 4 Young produced L 3 I Li 12r Iz.. J Adultmortality L-3.- s Young produced l4 1 l 1 1 t IX II Adult mortality L-l U l L l l 1 1 6 Young produced 1l

0.

4 0. Adult mortality l LT l L I l L IL I 7 Young produced [lZ 5 0 I LAQ-I 4 -I I Total yIoung produced Final Adult Mortality i 1 U i L l Note: Adult mortality (L - live, D - dead) i Concentration: % Mortality: 20l7. l l MeanOffspringFemale: l % Reduction from Control: 12.1 -.

Environmental Testing Solutions, Inc. Verification of Ceriodaphnia Reproduction Totals Control Day ~Replicate numberToa Day j 3 4-6 7 8 Total0 I 0 0I 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 000 0 0 0 0 0 0 0 0 4 33 5 5 54 5 5 4 5 5 46 5 1 2 10 13 10 9 10 9 11 12 10 106 6 13 0 0 0 0 0 I 0 16 141 44 7 0 14 17 17 17 15 I18 17 0 01 115 Total 28 -279 35 32 0303 32 331 291 311 600 mg NaCl/L_____ Day ~Replcate numberToa D I 2 3 4 5 6 7 9 oa I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 00 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 5 5 5 5 5 5 4 4 5 46 S 1 I 10 10 10 12 10 9 10 I1I 10 103 6 0 0 0 0 16 1141 0 0 15 15 60 7 17 13 16 160 0 18 14 0 0 94 Total 31 28 31 311 33 J291 32 28 30 30 303 800 migNaCVL. Day ~~Re Uicate numberToa 1 2 3 5 6 71 g-Tota 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 3 6 4 5 4 4 5 4 4 4 43 5 10 11 10 11 9 9 9 12 11 10 102 6 01 0 0 0 0 00 13 15 17 45. 7 15 15 17 141 1 160 0 0 106 '1otal 28 32 31 30 2 29 3 29 0 31 296 1000 mg NaCIIM DyRe pIlcate number Total Da 1 2 13 4 S 8 9 10 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4-5 3 S 6 4 4 4 4 43 5 9 9 10 10 10 8 1 0 8 1 1 I11 96 6 14 0 0 0 0 0 0 0 0 12 26 7 0 121 513 131 13 9 14 0 103 Total 27 22922872721 29 27 268 1200 mg NaICI/L___ Day Replicate number Total 12314 5 6 7 18 9 10 I 0 0 0 0 0 0 0 0 0 0 -0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 -0 0 0 0

• 0 4

4 4 5 4 4 3 3 4 4 3 38 5 8 6 6 4 10 8 7 7 4 4 64 6 0 0 0 0 0 0 0 0 4 6 10 7 6 0 0 9 74 5 500 36 Total 181 10 11 171 21 i15 15 161 12 1 4 1400 mtg NaC-- Day -R-p-j cate numberTotal I4 S 6 7 9 1 0 I 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 00O 0 0 0 4 4 3 2 3 4 3 0 2 02 23 5 7 -3 6 S 4 4 0 3 0 3 35 6 0 0-0 0 0 0 0 1 1 7 5 0 46340 4 0 0 26 Total 16 6 1 14 11I 11 0 9 0 6 85

Environmental Tcsting Solutions, Inc. Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1002.0) Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #I67 (N3 at 351 Depot St.) UTEX Algae Test datets: July 12-19, 2005 Revelwed by: r Concentration Rept_ e number I Survival Average reproduction

• akA re dts.. (mm (mt/L NaCI) 1 2

3 4 5 6 7 T 9 10 (%) (offspringlremale) vsda (% Comml (%) Control 28 29 35 32 30 30 33 32 33 29 100 31.1 7.2 Not applicable 600 31 2S 3 1 31 33 29 32 28 30 30 100 30.3 5.4 2.6 800 28 32 3 1 30 26 29 30 29 30 3 1 100 29.6 5.8 4.8 1000 27 25 29 28 28 27 27 2 1 29 27 100 26.8 as8 13.8 1200 18 10 I r 17 21 i s Is 16 1 2 1 3 100 14.8 22.9 524 1400 16 6 12 14 0 0 65 643 727 Dunnett's MSD value: PMSD: 3.165 10.2 MSD - Minimum Significant Difference PMSD - Percent Minimum Significant Difference PMSD Is a measure o'test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant In a whole effluent toxicity test. On avenage, i significant difference occurs for Environmental Testing Solutions, Inc. chronic toxicity tests when a toxicant reduces CerfodapAnla reproduction by 9.2% from the control. LowerPMSDbounddetermined byUSEPA (10' percentile) - 11%. Upper PMSD bound determined by USEPA (90* percentile) - 37%. The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cerfodaphnka reproduction in chronic reference toxicant tests. USEPA. 2000. UnderstandingandAccountingforMethod VariabilityinWholeEfuentToxicityApplicationsUndertheNationa PollutantDischargeElimin aonProgram. EPA-833-R-0-003. USEnvironmentalProteaon Agency, Cincinnati, Ofll

Environmental Testing Solutions, Inc. Statistical Analyses Cededspo *la S rvival and RepredvetUv Tel-jRepredvtles Stut Dte: 71122005 Tat HlY C4NaCIR Sampl D MI-RBF.RfToxzUl End Dte: 7 51912005 lab ID: ETS-Eav. Teatig Sol 3 UmpID Type: NACLSodiun Chtido Saple Dnte. Protocol FWCHIRB-PA-121.R402-013 Toot Specees: -Caerbdapbnh dubia Comment,: C.e-mig/L I 2 3

• 7 9

10 D-Costmi 23.000 29.000 35.000 320ooo 30.000 30.000 33.000 32.000 33.000 29.000 600 31.000 21.000 31.000 31.000 33.000 29.000 32.000 23.000 30.000 30.000 000 21.000 32.000 31.000 30.000 26000 29.000 30.000 29.000 30.000 32.000 1000 27.000 25.000 29.000 23.000 23.000 27 000 27.000 21.000 29.000 27.000 1200 13.000 10000 I1.000 17.000 21.000 15.000 3000 15000 12.000 13.000 1400 26.000 6000 12.000 I4.000 11.000 11000 0000 9.000 0.000 6.000 Theeftrnu Ustmatstoed Rtak -Tlated Isetank Come-3t L Me.a N-MS.A Man Min MAX CV% N Sozu CyIddl Meo N-Men D-C=01 31.100 1.0000 31.100 21.000 33.000 7.132 1o 31.100 1.0000 600 30.300 0.9743 30.300 21.000 33.000 5.401 10 95.00 75.00 30.300 0.9743 Soo 29.600 0.9513 29.600 26.000 32.000 5.736 10 37.50 75.00 29.600 0.9510

• 1000 26.000 0.1617 26.800 21.000 29.000 1.760 10 60.00 73.00 26.100 0.3617 11200 14.000 40.739 14.100 2o.0oo 21.000 22924 10 3300 73.00 14.000 0.4739 21400

.S00 0.2733 3.500 0.000 16.000 64.259 10 35.00 7S.00 3.500 0-2733 AnxlIary Tests Statistic Critical Skew Kert KoknohorDTat icatea mealdsntbtion(p>0.01) 0.99586133 1.033

.0.4676473 1.52342436 Bartt Tett sdiCtat tutequal v e (p - 1.1511-3) 20.20035U4 1.0862722 llypthesis Test (-lI. 0.5S) NOWC LOAC CV T]

Ste, Many-On. Rank Test 300 1000 894.427191 Treatmets w I trol Uesar lsterpebtloe (200 Rosamples)

P.1st ting/L SD 9b% CL Skew ICOS 303.92357 125.04005 440.176515 398.220443 -1.1130 ICIO 913 53.0220931 113.34328 2003.03634 0.3160 ICIS 1006.08333 29.7439229 923.373336 1032.54913

• 1.2221 1.0 I0 2032 13.1763532 1000.23143 1057.05037

-0.5002 0.9 IC25 1057.91667 13.9219323.1029.73462 10337967 .0.2323 IC40 1135.66667 14.3046107 1108.67963 1164.35622 0.1112 0.3 Icso 3137.5 18.1570691 113d.93744 1232.04375 0.5453 0.7 0.6 C 10.5 0.4 0.3 0.2 0.1 0.0 0 500 1000 1500 Dos* agliL De-Respenee,lMt 07-12-05new

Environmental Testing Solutions, Inc. Statistical Analyses Cerfodaphala, Survival and Reproduction Test-Repoduction Sart Date: 7112r205 Test ID: Cd&SC1CR Sanpile ID: REF-RefToxicant End Date: 7/19/2DOS Lab D: ETS-Eavir. Teting SaL Sample Type: NACL-Sodiun dkcxide Sacle Date: Prctocol: W FWCiR-EPA821.R.02-013 Test Species: CD-Ccriodsphnis dubis Coae-mg/L 1 2 3 4 5 6 7 a 9 10 D-CcAtrO1 28.000 29.000 35.000 320D0 30.l00 30.000 33.000 32000 33.000 29.000 600 31.000 28.000 31.000 31.000 33.)00 29.000 32000 28.000 30.000 30.000 BOO 28.000 32000 31.000 30.000 26.000 29.000 30.000 29.000 30.000 31.000 1000 27.000 25.000 29.000 28.000 28.000 27.000 27.000 21.000 29.000 27.000 1200 18.000 10.000 11.000 17.000 21.000 15.000 15.000 16.000 12.000 13.000 1400 16.000 6.000 12000 14.000 I1.O0 11.000 0.000 9.000 0.000 6.000 Tmansfoar: Untrmanormed I-Tailed Come-mg/L Mean W-Mean Mean Mi Max CV% N t-stat Critical MSD D.Ccitrol 31.100 1.0000 31.100 28.000 35.000 7.182 10 600 30.300 0.9743 30.300 2S.000 33.000 5.401 10 0.578 2287 3.165 800 29.600 0.9513 29.600 26.000 32000 5.786 10 1.084 .287 3.165

• 1000 26.800 0.8617 26.800 21.000 29.000 8.760 10 3.107 2.257 3.165
• 1200 14.800 0.4759 14.100 10.000 21.000 2Z924 10 11.778 Z27 3.165
• 1400 8.500 0.2733 8.500 0.000 16.000 64.259 10 16.331 Z2Z7 3.165 AniliaryTests Statistic Critical Skew ICrt KildmbxaoD Test indicates narnal distribuiticn (p > 0.01) 0.99586135 1.035 40.46764754 1.52342436 Brtletts Teat indicates anequal vmriuw (p - 1.15E43) 20.2003574 15.0862722 Hypothesis Teat (I-lI, 0.05)

NOEC LOEC CkV TU MSDu MSDp MSB MEE F-Pmb df DioEtfaTesa Soo 1000 t94.427191 3.16452289 0.10175315 905.576667 9.57592593 1.9B-25 5.54 Treatments vs D-Ccitrol 07-12-O5rew

Environmental Testi-ng Solutions, Inc. Page 5 of 6 Environmental Testing Solutions, Inc. Page 5 of 6 Species: Ceriodaphnia dubia IJ AL6Ae. CdL-aCLCR #: Z38 Daily Chemistry: Dav _~~Analyst VJE%5__Y 1 Concentration Parameter pHI (S.U.) -1.9\\ SB -l 5O §lR~ DO (mg/L)

8. c I.6 8.O

.O CONTROL Conductivity (pzmhos/cmn) 0 Alkalinity (mg CaCOlL) Hardness (mg CaCQ 3/L) Temperature _____(°C) 24.(S 2tl,A 2L.1 ZS.O 'v4A. uoeq.5 pH (S.U.) -1:8S? t#0)q) DO(mg/L) .'i __0 g.!5 600'mg NaC/L Conductivity (jPmhos/cm) _4__ Temperature 2S.2... 2el.S pH (S.U.) 1.79 _.? _____2_ DO (mg/L) R1_ _11l 800 mg NaCVL Conductivity (pimhos/cM)__ Temperature ?A2-7 2q.1

7. $

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6-)Xt.1 -l.9 8-O 4.%o 1200 mg NaCllL Conductivity 21(cO Z&01-zc eo tpmhos/cm) _ Temperature Zq. q7 (°_) _lA _S -2q._I 2Y1 CC7 Z pH (S.U.) U.1i 3.3-0 -- 4q 1400 mg NaCIJL DO (mglL) l.95 -W I i 7 I A-Conductivity 3SO0Z520CIM Igmhos/cm) I. Temperature (°C) 72q. S 274. %1l.1 2q. 7.q.S I STOCK Conductivity I (,mhoslcm) 136oD _rom IJ - I - J Initial I Finadl Initial Final Initial Final Tnitinl Finni Initial Final I.

Environmental Testing Solutions, Inc. Page 6 of 6 Environmental Testing Solutions, Inc. Page 6 of 6 Species: Ceriodaphnia dubia IlA3 IL5Ae. CdNaCLCR #: I. Day I 3 I 5 1 6_ t1rD l lt-rl° 11 aZV I t Analyst I a IJA0 I Concentration Parameter nH (S.U.) I -7.G 1 1h 9 1 qi.2 1 7.71 1 -7.Ba-1 r_ \\_._, g DO (mg/L) &O 1 I 411 1-4 I II ]7.7 1 CONTROL Conductivity (gmhos/cm) 301 Alkalinity (mg CaCOAL) Hardness (mg CaCO3/L) W;o &I 3i5 38c 1u; 2 8 2- &(0 Temperature (°C) I 7ZS A VA A -24A 2zq W-1 un 24.9 pH _S.U It. DO (msz/) 'I r n.17i 7.9 A % 7.q s e 600 mg NaCUL Conductivity jq57 (i4mhos/cm) MILO___ Temperature ?A. 5 2ql. Z - bzA Z..A-

• 'IC) 7.AA. T laz. r-g2 pH (S.U.)

IS Ig f.° -/A0 -7.7 1 A= -7.?,ZG DO (mgtL) e D1.7 o 7.7 1. 800 mg NaCVIL Conductivity i __1_l Temperature Z0 7l z 24G.E P z7a L ( 0 C .pH (S.1 T a 77 71 7U.. DO(gL) .il l1;

n.

7-7 1000 mg NaCVL Conductivity l240 2z O (.unh s/cm ) _____0 Temperature 21-1 ZS.1 Z. ZLI. 24.9 (0 c) pH (S.U.) ?.N t xff7 7< 1 ;7 7 DO (mg/L)

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.7 1.1 1 0 ° I7. 1200 mg NaCLL Conductivity Trathosucm) _b.A_ 2(O-0Z ZoA_ 290 Temperature 242 4*

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-I 2A. ( 0C ) nIT tS.TUA ls 7-r -f1\\ hn 2 -7 1 4 -1. _P. J -- I _- ZRL I t P k I I N j V - DO (m-/LI 810 i -,q I 117 1.7 R. I -7.9 WR1 1 1400 mg.NaCVL Conductivity 30q0 (plmh s/cm) I 8000 (on 2, VC l ag auso l Ark U-.---, Temperature (00C 2q.1 zS.O I 2'q. G -ST. I N4.- S 2L1.9 Z' It I --.. I - STYUCK Conductivity (umhosfcrnM ,T. a '- 91 Initiail Final I TIntial Initial] IFinal I Initial Final A.....".~~~~ An. M I..... u^.,..._ iii 113 I Init. I ..~.. ih.ss.H rin--l I Initia

PERMITTEE NAME/ADDRESS (Include FacilityName/locatlon iDifferent) Name TVA - SEQUOYAH NUCLEAR PLANT________ Address P.O. BOX 2000 SODDY. DAIY__ TN 37384 ady_ TVA-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (OMR) TN0026450 l 101 Y I I PERMIT NUMBER I DISCHARGE NUMBERl o R MO DAY ToYEAR I MO From 04 108 I 01 ITo 05 l 07 31 MAJOR Form Approved. (SUBR 01) OMB No. 2040-01 F - FINAL ANNUAL MONITORING (PCBS) EFFLUENT

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PERMITTEE NAMEIADDRESS (Include Fac7iityName/tocatIon IfDifferent Name TVA -SEQUOYAH NUCLEAR PLANT - Address P.O. BOX 2000 _____ __ _ _1NTEROFCE SB-SODDY - DAISY JN 37384 Fad!y 'VA SEOUOYAH NUCLEAR PLANT Location _AMITNqCOUNT?.._._.. NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form DISCHARGE MONITORING REPORT MDMR) (SUBR 01) 0MB I TN0O60 103LG F - FINAL PERMIT NUMBER I DISCHARGE NUMBER LOW VOL WASTE TREATMENT POND IONITQRINCEFRI IEFFLUENT IYEARI MO - II MO I DAY In .n. i... Approved. 4o. 2040-0004 ATTN: Stephanie A. Howard From [-05 l 07 I 0o-1 To I 05 1 07 l 31 1 -- NT. Ul0%RadIntr b r m n NOTE: Read instnictons before compfetinq this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE E X o f: T Y P E AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 6.8 8.7 12 0 13131 GRAB MEASUREMENT 00400 1 0 0 PE M Y e LB BY 'O 6W 0L MTHREE G: RAB MEASUIREMENT, EFFLUENT GROSS VALUE REUR M N M N M Mu~MA I U W E SOLIDS, TOTAL SUSPENDED SAMPLE 103 118 26 12 13 19 0 4131 GRAB MEASUREMENT 00 5 E M T-j ~ ~ 1 0 ~ 5 ~ 7 - LBSIDY MGL

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PERMITTEE NAMEIADDRESS (Include Facilitv Name/locatlon if Different) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 ___(I EROFFIEES-EB )_ -F- -CES SODDY - AISY TN 37384 FaqlL TVA-SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR01) Form Approved. OMB No. 2040-0004 l i I TN0026450 I PERMIT NI j1 107 G F - FINAL I I DISCHARGE NUMBER I METAL CLEANING WASTE POND MOlNITORlING PEpIODl I YEAR MO I DAY I MO DAY From I05 107 l 01 I ToI 05 107 l 31 EFFLUENT -* NO DISCHARGE [3] *** NOTE: Read Instructions before comnletino this form. ATTN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE ______________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 12 00400 1 0 0 PEQR4MIT> yM,,Ž'.U, SU RA REQUIREMENT 1G EFFLUENT GROSS VALUE .M. IN

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W v1 ~.... MGL DAILY. COMPOS MREQUIREMENT 197 EFFLUENT GROSS VALUE Y W M G L DAIY X SOLIDS, TOTAL SUSPENDED SAMPLE 1* MEASUREMENT 19 00530 1 0 0 iCERMEM 7 I fm ' l,,"z MGL -* D C EFFLUENT GROSS VALUE ~ -01 VK - ~DAI LY M OIL AND GREASE SAMPLE MEASUREMENT 19 00556 1 0 0 r '-N MG MPDS EFFLUENT GROSS VALUE k ~.-*. AL X COPPER, TOTAL (AS CU) SAMPLE 19 MEASUREMENT P9 01042 1 0 0 P.-MGIL g AILY M, COMPOS ',REQiJIREME1 ~ -~ '~ ~,w~r EFFLUENT GROSS VALUE DALY IRON. TOTAL (AS FE) SAMPLE

• ee 1

MEASUREMENT 1 01045 1 0 0 iPERMIT-..~ .. ~ ae*. 1-EFFLUENT GROSS VALUE REUIEEN4 .DAIL1TO GI DILMXMO FLOW, IN CONDUIT OR THRU SAMPLE TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 ~ ':PRI,~"EORT,; ~- PORT-'I' MGD "DAILY-' 7 CALCTD' REQUIRENj y EFFLUENT GROSS VALUE AMAG ALYMX ___'h,___ NAMEITITLE PRINCIPAL EXECUTIVE OFFICER I Certify uwider perialty of low that this dowmener and all attscments were prepared under my dil ~TELEPHONE DATE directon or supervisIon In accordance wlth a system designed to assure that qualified personnel J c_ J. Randy Douet proierly gather and evaluate the Information submitted. Based on my hquIry of the person or persons who managethe ystenoirtho@epersonscbtr renporsl e forgathering the Principal Environmental Engineer 423 843-700 05 08 10 StViePedetInformatin, the Information submitted Is

• to fth best of my Inowledge and bellef, true.

423__843__6700___________ S Pacrate, and complete. I am aware tha therUe are sIgnifIcant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TPED OR PRINTED nformation, Icltding the possibility of trie and Imprisornment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA INUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VOLATIONS (Reference all attachments here) No Discharge this Period LI' t'ot JiZ-(H* V JIM Preiou edtin may Ae use Page.. 1 of__ 1_ U-A I-Onn JJZU-11 (KF-V 3199) Prewous ecfMons may be USed Page 1 of I

PERMITTEE NAMEJADDRESS (tncfude FacilityName/Location fDifferent) Name __TA - SEQUOYAH NUCLEAR PLANT __ Address P.O. BOX 2000 ____LNF~tOECESB-- SODDY - DAISY TN 37384 djy__ TA-SEQUOYAHNUCLUEAR LANT7 Location _AMILTON COUNTY NATIONAL POLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR DISCHARGE MONITORING REPORT (DMR) (SUBR 01) I TN0026450 110 G I F-FINAL I PERMIT NUMBER I DISCHARGE NUMBER IRECYCLED COOLING WATER Form Approved. OMB No. 2040-0004 MONITORINGQ PFRIOD I EFFLUENT From l II YEAR I MO I DAY From 05 0 l01lTo I05 1 07 l 31l ATTN: Stephanie A. Howard ` NO DISCHARGE F1 *1 NOTE: Read Instrucons before completing this form. PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 12 MEASUREMENT 00400 1 0 0 Su u U ,DAILY ,-GRABL, EFFLUENT GROSS VALUE

REOUIREMENT4Vi

,.."6. ~ .~ EFFLUENT GROSSMVA-UE i MAXIMUM SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT EFFLUENT GROSS VALUE U_ _v iw. S MGL A COMPOS OIL AND GREASE SAMPLE 1******* e*a***9 MEASUREMENT 00556 1 0 0 PERMIT. aa*' e MGIL -i DAILY,; COMPOS EFFLUENT GROSS VALUE AISOUIREMEN' ~ -~ tI~ COPPER, TOTAL (ASCU) MESURMPEN 19 01042 1 0 0 .PRE0IRE~ H '-- _aeae'~ .r MGIL*e~.. _~* m COMPOS~eae EFFLUENT GROSS VALUE <*<~_______' , -iDAILY /X IRON, TOTAL (AS FE) SAMPLE 19 MEASUREMENT 01045 1 0 0 -~PER rZ:f ~ ~* ~f* ,~ GL~-DAILYtz COMPOS EFFLUENT GROSS VALUE E . u MXs __COMPOS PHOSPHORUS,TOTAL (AS P) SAMPLE 19 MEASUREMENT 1 00665 1 0 0 A,'PERMIT. D:I MGL

• .h DAL COMPOS EFFLUENT GROSS VALUEREURMN DALMX FLOW, IN CONDUIT OR THRU SAMPLE 19***

TREATMENT PLANT MEASUREMENT 03 50050 1 0 0 R ORT MGD n rCALCTD EFFLUENT GROSS VALUEREURMN NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certity under penalty of law that this documernt and aln attadrients were prepared under my / / TELEPHONE DATE direction or supervision In accordance with a system designad to asswa tha"t qaiifd prsonnell ) d J. Randy Douet ipropery gther and evalue the Iformation submitted Based on my inquiry of the person or '-5 Sipersons who manae the system or those persons desdl responsible for gat the Principal Environmental Engineer 423 843-6700 05 08 10 S__e Vice President accuate. and CompIete. I am eware that thare are significent penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE T Pn aflonn Including t possnbnnydfine and Irisonment foraknowingfviolationte OFFICER OR AUTHORIZED AGENT ARt-A INUMBER YEAR MO DAY TYPED OR PRINTED 1 CODE COMMENTS AND EXPLANATION OF ANY ViOLATiONS (Reference af fattachments here) No Discharge this Period EPA FOrM 3320-1 (REV 3199) Prevtous efforts maybe used Page 1 of I

PERMITTEE NAMEIADDRESS (Include FacilityName/Location ifDifferent) Name TVA - SEQUOYAH NUCLEAR PLANT ____ Address P.O. BOX 2000 - _LJNTEROFfCE SBMM. SODDY DAISY_ TN 37384 FadjN_ TVA - SEQUOYAH NUCLEAR PLANT Locca.on _AMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) l TN026450 l 116 G l PERMITNUMBER 1D=ISCHARGENUMBER I EONITLQRING PER1OU l MAJOR (SUBR 01) F-FINAL BACKWASH EFFLUENT Form Approved. OMB No. 2040-0004 YEAR I M +/- A From 05 i 107 l °01<-1 TO YEAR I MO DAY NO DISCHARGE = To 05 l 07 31 NOTE: Read Instructions before ComDietna this form. PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREQUENCY SAMPLE _EX OF I TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1131 VISUAL MEASUREMENT 01345 1 0 0 PERMIT;....... REPORTi. PASSX m mSEE ISUAL OEQUIEMENT EFFLUENT GROSS VALUE ?ROURMET FA

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~ M ',T O0TA L,' F I I PE M ITr ~ OIL AND GREASE VISUAL SAMPLE 0 94 0 1131 VISUAL MEASUREMENT 84066 1 0 0 -; PERMTORT YES=1 i E R M'-IT SEE -'VISUAL, M;EASUREMENiT-EFFLUENT GROSS VALUE RURMET OTTA" NO=0 ____PRI SAMPLE MEASUREMENT x REQUIREMENT,'i 7. Hi SAMPLE MEASUREMENT 5Q, R UIREMENe. SAMPLE MEASUREMENT

• RiEQUIREMENT~

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• EUIREMEN1

'*r e,4 Ca NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this docznent and an attEdcments we prepared under my TELEPHONE DATE direction or supervision In accordance with a syslem designed to assure that qualifed personnel 1 V //.. d J. Randy Douet properly gather and evaluate the Itformtallon submitted. Based on my hiquiry of the person or / Io persona who manag tft system, or those persona directly responsible for gatherkng ft Principal Environmental Engineer 423 843-6700 05 08 10 Site Vice Pesident ltfOI7T~b~n*the Inometlon submitled Is. to fte best of my knowedge end belier, true, _____________I eacrale, and complete. I am aware that there are slgnificant penalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE TEORmPtion, hIclidng the pssiblitly of fine and Imprisnment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ___________________________________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference alt attachments here) Operations performs visual Inspections for floating debris and oil and grease during all backwashes. ~rP. Drkm.53eU* 4r~ Knee i-"evs A.._AXilos may a..... iS__ EPA ru0 3J=Us1 I tKCV 319S) 1-mvious edirlons may De used Page 1 of 1

PERMITTEE NAME/ADDRESS (Incrude Facl7ity Name/tocatin IfDifferent) Name _ TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 _ _- £EROFFIE .SB-2 )__________ SODDY-DAISY TN 37384 Lacy_ WA SEOUOYAH NUCLER PLANT Location HAMILTON COUNTY ATTN: Stephanie A. Howard NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) DISCHARGE MONITORING REPORT (DMR) l TN0026450 1 117 G I PERMIT NUMBER I I DISCHARGE NUMBER MONIT o vER10 YEAR IMO I DAY IYEAR IMO i DAY From 05 Osl 07 l 01 ToI 05 107 l 31 MAJOR (SUBR 01). F - FINAL BACKWASH EFFLUENT Form Approved. OMB No. 204040004 NO DISCHARGE W NOTE: Read Instructions before comlletrin this form. PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREOUENCY SAMPLE ____EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS DEBRIS, FLOATING (SEVERITY) SAMPLE 0 9A 0 1 31 VISUAL MEASUREMENT 01345 1 0 0 RMO R ORTA PASSO 0 f a'EE VISUAL: EFFLUENT GROSS VALUE

q.

.70____ ~ AOTTAL-'FI1 'Y PRI OIL AND GREASE VISUAL SAMPLE 9****°4 0 0 1 /31 VISUAL MEASUREMENT 84066 1 0 0 ~ PERMI~t`'-~- ~ ~RPRp E= ~SE VISUALr MEASUREMENT MEASUREMENT EFRLUENTEGROSSV.A. LUE QTAC 'PRMT SAMPLE MEASUREMENT SAMPLE MEASUREMENT. L ~W~ R.* -. 'REQUIREMENT j .:E SAMPLE MEASUREMENT REQUIREMENT P NAlifErITTLE PRINCIPAL EXECUTIVE OFFICER I Certify wider penalty of lawv that thIs doaznent and all attsclwents wers prepsfed unider mny ,/J d TELEPHONE DATE dirertion or supervisnion e, ccordane with a system desugned to assure that qulified personnel At t ({'4Y ) _t.L J. Randy Douet operotyat 8her snd evaluate the Wnormstbon submitted. Based on my Inuwy fd the persori or I .I pebrson wthe manage the sys~tem is t h bns dtrectly responible horgatherh9g the Principal Environmental Engineer 423 843.5700 05 08 10 Site Vlice President aruae an Ispif ea tha ther bres of myan pedgtes hr~ ubmflri iae SIGNATURE OF PRINCIPAL EXECUTIVE II rmand OFFICER OR AUTHORIZED AGENT l UER YEAR MO DAY COMMENTS AND EXPLANATtON OF ANY VIOLATIONS (Refference aDl attachments here) Operations performns visual inspections for floating debris and oil and grease during all backwashes. EPA orm33201 (EV 399) PrevouseditonsmabENuedPae-'o EPA Form 3320-1 (REV 3199) Previous editions may be Used Page I of 1

PERMITTEE NAME/ADDRESS (Include FadlityName/locatlon IfDfferent) Name TVA - SEQUOYAH NUCLEAR PLANT Address P.O. BOX 2000 -_( 1,fl ER 0F FCE-EQ -B -- -- - SODDY - DAISY TN 37384 Fadi_ TVA-SEOUOYAH NUCLEAR PLANT Location HAMILTON COUNTY NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved. DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 l TNO4 5O F-118 F - FINAL I PERMIT NUMBER DISCHARGE NU BER WASTEWATER & STORM WATER I MONITQRINC' PFRIQQ 1 EFFLUENT YEAR I MO I DAY IMO I DAY From I05 I07 l 01-Tol 05 107 l 311

• • NO DISCHARGE [

NOTE: Read Instructions before completing this form. ATrN: Stephanie A. Howard PARAMETER QUANTITY OR LOADING QUAUTY OR CONCENTRATION NO. FREOUENCY SAMPLE ______________________EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE 19 MEASUREMENT 00300 1 0 0 PERMS. by*4

d. ;0?w *Wi:A EFFLUENT GROSS VALUE FEUIEMNT Zlr DAIL;Y MILMTIE/

RA SOLIDS, TOTAL SUSPENDED SAMPLE 19 MEASUREMENT 00530 1 0 0 J;fr"PERMI'~~'****.~****:~

• • • • f.'

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SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 0 PERMIT.'..e; i.L**--a----. dO N MLIL ON -GRABH. 'REfQUIREMENT-- ',%',\\-.-'DAL Mn t.MOTHs EFFLUENT GROSS VALUE DALY IN'

• NTH:

FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 50050 1 0 0 PR, -V,,', P Rr- ,.R-EP MGD Ax

• .ONCE/,

'ESTIMA; SAMPLE_ MEASUREMENT SAMPLE MEASUREMENT T ;;REOT -,(,,; SAMPLE MEASUREMENT 'RE0UIREMENT. SA~Y~ i -~'*.. "~ NAME/TTLE PRINCIPAL EXECUTIVE OFFICER I Certify tader penalty of law that this Panzwrt and all attacints wexe prepared taider myr /' i TELEPHONE DATE hdireton or aupervislon hi accordacnce witlh a system designed to Basuns that quaslified personnel ~j7'M.jjp i~ (jV1OVCA J. Ra ndy Douet properly gather and evaluate the Information sutbmtted Based on my Inquiry of the person or A.,~ idpersonswhomsnagethesytemothiosetptsotn'sdlratyirX~lfesoe ndstiblefrgthethPrlncdpal Environmental Engineer 423 843-6700 05 08 10 Ste Vice President s acanate, and complete. I emra ware that there are siganificant pvenalties for submitting false SIGNATURE OF PRINCIPAL EXECUTIVE information, Inrudingtheposstbllityof fine and tmiortsomrrentfor~ioowingviotatlons. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _CODE COMMENTS AND EXPLANATION OF ANY VIOLARIONS (Reference aft attachments here) During this reportng period, there has been no flow from the Dredge Pond other than that resulting from rainfall. EAFr3301(E399 Prvoseitrmaions thye usedto umttdI.t ebs o ykoldead eif re 2 Page70 15 of 1 III EPA Form 3320-1 (REV 3199) Previous edfflons may be used Page 1 of 1}}