ML20079N038

1989 Annual Environmental Rept Non-Radiological Duquesne Light Co,Beaver Valley Power Station Units 1 & 2

ML20079N038
Person / Time
Site:	Beaver Valley
Issue date:	12/31/1989
From:	Cody W, Mcintire J, Shema R AQUATIC SYSTEMS CORP., DUQUESNE LIGHT CO.
To:
References
RTR-NUREG-1437 AR, NUDOCS 9111110055
Download: ML20079N038 (158)

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t 9 1989 AHHUAL ENVIRONMENTAL REPORT H0H-RADIOLOGICAL DUQUESHE LIGilT COMPANY DEAVER VALLEY POWER STATION UNITS HO. 1 & 2 t 8 .e

w s 1989 ANNUAL ENVIRONMENTAL id: PORT NON-RADIOLOGICAL DUQUESNE LIGirr COMPANY DEAVER VALLEY POWER STATION UNITS NO. 1 & 2 Prcyst06 by' Pabe r t 1.ou i n ' th a e W1111am H. Cody 4 Gary J. %erderes Michael P. :avison Michael R. Noel Gregory M. Styborski Aquatic Systems Corporatlon Pittsburgh, Pennsylvania and Donald S. Cherry, Ph.D. Virginia Polytechnic Institute and State University Blacksburg, Virginia and J. Wayne ficIntire Duquesne Light Company Shippingport, Pennsylvania 4

Out TABLE OF CONTENTS Page r LIST OF FIGURES............................................. iv LIST OF TABLES.............................................. Vi 1 I. INTRODUCTION.... A. SCOPE AND OS' TW1'd C7 'f.' PROGRAM..................... 1 2 B. SITE DESCRIPT;c... II.

SUMMARY

AND CONCLUSIONS..................................... 8 III. ANALYSIS OF SIGNIFICANT s3NIRONMENTAL CilANGE................ 13 IV. MONI'IORING NON-RADIOLOGICAL EFFLUENTS....................... 14 A. HONITORING CHD4ICAL EFFLUENTS........................... 14 B. HE RB I C I D E S.............................................. 14 V. AQUATIC H3NITORING PROGRAM.................................. 15 A. INTRODUCTION............................................ 15 f B. BENulOS................................................. 15 Objectives.......................................... 15 Methodu............................................. 15 !! a b i ta ta............................................ 19 Coamunity Structure and spatial Distr ibution........ 29 Comparison of Control and Non-Control S t a t i on s.......................................... 29 Comparison of Preoperational and Operational Data.............................................. 31 Summary and Conclusions............................. 35 C. PIITIO PI AN K' ION........................................... 35 Objectives.......................................... 35 Methods............................................. 35 Seasonal Distribution............................... 36 Comparison of Control and Non-Control Transects......................................... 41 Comparison of Preoperational and Operational Data.............................................. 41 Summary and Conclusions............................. 44 1

1 TABLE CP Colfre. HTS (Continued) Page e D. Z O3 PIAN KTON............................................. 44 Objectives.......................................... 44 Methods.......................................... 48 i Se a sonal Di s t r i bu t ion............................... 48 Compartson of Control and Non-Control Transects......................................... 56 Comparison of Preoperational and Operational Data.............................................. 56 Suxnary and Conclusions............................. 58 E. FISii.................................................... 61 Objective........................................... 61 Me th od s............................................. 61 Results............................................. 63 Comparison of Control and Non-Control Transects......................................... 69 Comparison of Preoperational and '.perational Da t:4.............................................. 75 Summary and Conclusions............................. 75 F. I Cli TH YO PIAN KT0 N......................................... 78 Obj e c t i v e........................................... 79 Metheds............................................. 70 g Results............................................. 80 i Comparison of Prooperational and Operational Data.............................................. 80 Summary and Conclusions............................. 85 G. P I S H I MP I N GEHr N T........................................ 85 0bjective........................................... 85 Me thod s............................................. 85 Re s u l t s............................................. 85 Comparison of Impinged and River Fish............... 90 Comparison of Operating and Non Operating I n ta k e B ay Co11ec t ions............................ 102 Summary and C9nclusions............................. 102 H. PIAN K' ION ENTRA I NMENT.................................... 103 1. Ichthyop1ankton..................................... 103 Objectives.......................................... 103 Methode............................................. 103 Results............................................. 104 l. Seasonal Distribution............................... 104 l Spatial Distribution................................ 104 Summary and Conclusions............................. 109 i l l l, t 1 I 11

a. TABLE OF CONTENTS (Continued) Page H. PLANMON ENTRAINMENT (Cont'd) 2. Phytoplankton....................................... 109 Objectives........................... 109 Methods............................................. 109 Compar ison of Entrainment and River Sample.......... 109 Summary aid Conclusions............................. 110 3. Zooplankton......................................... 110 Objectives.......................................... 110 Methods............................................. 110 Comparison of Entrainment and River Samples......... 111 Summary and Conclusions............................. 111 1. Corbicula MONITORING PROGRAM............................ 112 I n t r od uc t i on........................................ 112 1. Monitoring.......................................... 112 Objectives.......................................... 112 Me th od s............................................. 114 Results............................................. 117 Summary............................................. 127 e a 2. Growth Study........................................ 132 0bjective........................................... 132 Methods............................................. 132 Resulta............................................. 134 Summary............................................. 134 3. Spawning Study...................................... 134 Objective........................................... 134 Me th od s............................................. 134 Results............................................. 135 Summary............................................. 137 4. Larvae Study........................................ 137 0 bj e c t i v e........................................... 137 Methods............................................. 137 Results............................................. 138 Summary............................................. 143 VI. REFERENCES.................................................. 144 iii 1 t

l LIST OF FIGURES FIGURE Page I-1 VIEW OF Ti!E BEAVER VALLEY POWER STATION, DVPS............. 3 1-2 LOCATIQ1 OF STUDY AREA, DEAVER VALIEY POWER S TAT I Otl, S ilI P P I N G PO RT, P EN N S Y LVAtl i A....................... 4 I-3 0:110 RIVER FLOW (cfs) AND TEMPERATURE (OF) RECORDED BY Tile U. S. ARMY CORPS OF ENGINEERS FOR Tile NEW CUMBERLAND POOL, 1989, DVPS............................... 5 V-A-1 SAMPLING TRAN9ECTS IN TifE VICINITY OF THE DEAVER V A LI2Y POWE R S T AT I ON S..................................... 16 V-B-1 D EN Th0S S AMPLI NG STATI OllS, DVPS........................... 18 V-D-2 MEAN PERCENT COMPOSITION OF Tl!E DENTilOS COMMUNITY IN THE 01110 RIVER NEAR DVPS DURING PRCOPERATIONAL AND OP E RAT I ON AL Y EA RS......................................... 30 V-C-1 MONTHLY PHYTOPLANKTON DENSITIT" IN Tile O!!IO RIVER DURING PREOPERATIONAL (1974-lbi5) AND OPERATIONAL (1976-1989) YEA RS, DVPS................................... 38 V-C-2 Pl!YTOPLANKTON GROUP DENSITIES FOR ENTRAINMENT SAMPLES, 1989, DVPS....................................... 40 V-D-1 MONTl!LY ZOOPLANKTON DENSITIES IN Ti!E 01110 RIVER DURING PREOPERATIONAL (1974-1975) AND OPERATIONAL (1976-1989) YEARS, DVPS................................... 50 V-D-2 ZOOPLANKTON GR'UP DENSITIES FOR ENTRAINMENT SAMPLES, 19 8 9, D VP S................................................ 54 V-E-1 F I S il S AMPLI NG STATIONS, DVPS.............................. 62 V-F-1 ICllTHYOPLANKTON SAMPLING STATIONS, DVPS................... 79 V-G-1 I N TAK E STRUCTU RE, DVPS.................................... 86 V-I-1 PHOTOGRAP11S OF Cor bicula COLLECTED, DVPS.................. 113 V-I-2 Corbicula MONITORING PROGRAM SAMPLING STATIONS OF THE LOWER RESERVOIR OF UNIT 1 COOLING TOWER, DVPS...... 115 V-I-3 Corbicula MONITORING PROGRAM SAMPLING STATIONS OF THE LOWER RESERVOIR OF UNIT 2 COOLING TOWER, DVPS...... 116 l l l l Iv I I

LIST OF FIGURES (Continued) FIGURE U2' V-1-4 Corbicula MONITORING PROGRAM SAMPLING STATIONS, 01110 RI VE R S YSTEM, DVPS................................... 118 V-I-5 APPROXIMATE POPULATIONS OF Corbicula IN UNITS 1 AND 2 COOLING TOWE,RS, DERIVED FROM SURVEYS CONDUCTED IN 19 8 6 T11ROUa 19 8 9, DVPS.,................................. 120 V-1-6 Corbicula MOHITORING PROGRAM SAMPLING STATIONS, INTAKE STRU( 4 RE, DVPS.................................... 123 V-1-7

SUMMARY

OF Corbicula COLLECTED PROM TIIE INTAKE STRUCTURE TRAVELING SCREENS DURING IMPINGEMENT SURVEYS, 19 81 TH ROUGli 19 8 9, DVPS................................... 131 V-I-8 RESULTS OF Corbicu}a, REPRODUCTION STUDY IN Tl!E INTAKE STRUCTURE AND UNIT 1 COOLING TOWER, 1989.................. 136 V-I-9 RESULTS OF Corbicula LARVAE STUDY SIZE DISTRIBUTION IN TiiE INTAKE STRUCTURE AND UNITS 1 AND 2 COOLING TOWERS, 1989, BVPS................................................. 142 F V .~

~ _ _. _ _ _. _ _ _ _ _ _ _ _ _ _. _ LIST OF TABLES TABLE

Page, I-1 OllIO RIVER PLOW (cf s) AND TEMPERATURE (OF)

RECORDED BY Tile U. S. ARMY CORPS OF DIGINEER3 FOR T!!E N LM CUMBERLAN D POOL, 19 8 9, BVPS........................... 6 V-A-1 AQUATIC MONITORING PIOGRAM SAMPLING DATES,1989, BVPS...................................................... 17 V-B-1 SYSTEMATIC LIST OF MACROINVERTEBRATES COLLECTED IN PREOPERATIONAL AND OPERATIONAL YEARS 1H Tile 01110 RI VE R N EA R B V PS........................................... 20 2 V-B-2 MEAN NUMBER OF MACROINVERTEBRATES (Number /m ) AND PERCENT COMPOSITI0tt OF OLIGOCilAETA, CllIRONOMIDAE, MOLLUSCA AND Orri!ER ORG1.NISMS, 1989, DVPS.................. 25 2 V-B-3 BDiTil!C MACROINVERTEBRATE DDISITIES (Humber/m ), MEAN OF TRIPLICATE FOR BACK CllANNEL AND DUPLICATE SAMPLES COI.LECTED IN THE MAIN CIIANNEL 01110 RIVER, MAY 23, 1989, BVPS........................................ 26 2 V-B-4 BENTil!C MACROIINERTEBRATE DDiSITIES ' (Humber/m ), MEAN OF TRIPLICATE FOR BACK CilANNEL AND DUPLICATE SAMPLES COLLECTED IN Tile MAIN CilANNEL 01110 RIVER, SEPTEMBER 14, 1989, BVPS.................................. 20 V-B-5 MEAN DIVERSITI VALUES FOR BENTil!C MACROINVERTEBRATES COLI.ECTED IN Tile 01110 RIVER, 1989, BVPS................... 32 2 V-B-6 BENTHIC MACROINVERTEDRATE DENSITIES (Number /m ) FOR STATION 1 (CONTROL) AND STATION 2B (NON-CONTROL) DURING PREOPERATIONAL AND OPERATIONAL YEARS, DVPS................ 33 V-C-1 MONTl!LY PilYTOPLANKTON GROUP DDiSITIES (Number /ml) AND PERCDJT COMPOSITION FROM ENTRAINMENT SAMPLES, 1989, BVPS................................................ 37 V-C-2 PifYTOPLANKTON DIVERSITY INDICES BY MlRb s*OR ENTRAINMENT SAMPLES, 1989, BVPS........,.................. 39 V-C-3 DENSITIES (Number /ml) OF MOST ABUNDANT PNn GPLANKTON TAXA COLLECTED FROM ENTRAINMDiT SAMPLES, JANUARY. TilROUGli DECEMB E R 19 8 9, DVPS............................... 42 V-C-4 PlIYTOPLANKTON DIVERSITY INDICES (MEAN OF ALL SAMPLES 1973 TO 1989) NLM CUMBERLAND POOL OF Ti!E OH I O RI VER, BVPS.......................................... 45 vi l l l l e-. r- ~.,--

M LIST OF TABLES (Continued) TABLE Page V-D-1 MONTHLY ZOOPIANKTON GROUP DENSITIES (Number / liter) AND PERCENT COMPOSITION FROM ENTRAINMENT SAMPLES,

1989, BVPS................................................

49 V-D-2 MEAN ZOOPLANKTON DENSTTIES (Number / liter) BY MONTH FROM 1973 TilROUGil 1989, 01110 RIVER AND BVPS............... $2 V-D-3 DENSITIES (Number / liter) OF MOST ABUNDANT ZOOPIANKTOli TAXA COLLECTED FROM ENTRAINMENT SAMPLES, JANUARY TilROUGli DECEMBE R 19 8 9, BVPS............................... S t. V-D-4 ZOOPLANKTON DIVERSITY INDICES BY MONTH FOR ENTRAINMENT S AMP LES, 19 8 9, BVPS....................................... 57 V-D-5 MEAN ZOOPIANKTON DIVERSITY INDICES BY MONTil FROM 1973 TilROUGH 19 8 9 IN THE 01110 RIVER NEAR BVPS.................. 59 V-E-1 FAMILIES AND SPECIES OF FISil COLL.CTED IN Tl!E NEW CUMBERIAND POOL OF Ti!E OHIO RIVER, 1970-1989, DVPS........ 64 V-E-2 NUMBER OF FISH COLLECTED AT VARIOUS TRANSECTS BY GILL NET (G), ELECTROFIS!!IllG (E), AND HINNOW TRAP (M) IN TIIE NEW CUMBERLP.ND POOL OF THE 01110 RIVER,

1989, BVPS................................................

67 V-E-3 NUMBER OF FIS!! COLLECTED PER MONTH BY GILL NET (G), ELECTROFISHING (E), AND MINNOW TRAP (H) IN THE NEW CUMBERLAND POOL OF Tile 01110 RIVER, 19 89, DVPS............. 60 V-E-4 NUMBER Of FISil COLLECTED BY GILL NET, ELECTROFISilING AND MINNOW TRAP AT TRANSECTS IN THE NEW CUMBERLANO POOL OF TILE OHIO RIVER, 1989, DVPS........................ 70 V-E-5 ELECTROFISHING CATCil MEANS (R) AT TRANSECTS IN Tl!E NEW CUMBERIAND POOL OF Ti!E 011I0 RIVER, 1974-1989, BVPS........ 71 V-E-6 GILL NET CATCil MEANS (!!) AT TRANSECTS IN TiiB Hl W CUMBERIAND POOL OF TIIE 01110 RIVER, 1974-1989, DVPS........ 76 V-F-1 NUMBER AND DENSITY OF FIqt! EGGS, LARVAE, JUVENILES, AND ADULTS (Humber/100 m*) COLLECTED WITil A 0.5 m PIANKTON NET IN THE 01110 RIVER BACK CllANNEL OF PHILLIS ISLAND (STATION 2B ) NEAR BVPS, 19 89............... 81 3 V-F-2 DL:NSITY OF ICHTHYOPLANKTON (Number /100 m ) COLLECTED IN THE 01110 RIVER BACK CilANNEL OF PilILLIS ISLAND (STATION 28) NEAR BVPS, 1973-1974, 1976-1989.............. 84 vil

LIST OF TABLES (Cont.inued ) TABLE Page V-G-1 FIS!! COLLECTED DURIi1G T11E IMP 1NGEMENT SURVEYS, 1976-1989, DVPS........................................... 87 V-G-2 SUI MARY Of FIS11 COLLECTED IN IMPINGEMENT SURVEYS CONDUCTED FOR ONE 24 ILOUR PERIOD PER WEEK DURING

1989, BVPS................................................

89 V-G-3

SUMMARY

OF IMPINGr.MD1T SURVEYS DATA FOlt 1989, DVPS........ 91 V-G-4

SUMMARY

OF FISil COLLECTED IN IMPINGEMINT SUIWEYS, 1976-1989, DVPS........................................... 93 V-G-5 NUMBER AND PERCD1T OF ANNUAL TOTAL GF FIS!! COLLECTED IN IMPINGEMENT SURVEYS AND IN THE NEW CUMDERLAND POOL OF TIIE 01110 RIVER, 1989, DVPS............................. 95 V-G-6

SUMMARY

OF CRAYFIS!! COLLECTED IN IMPINGEMENT SUINEYS CONDUCTED FOR ONE 24-1100R PEF.IOD PCR WEEK, 1989, DVPS..... 96 V-G-7

SUMMARY

OF Corbicula COLLECTED DURING IMPINGEMDIT SURVEYS FOR ONE 24-HOUR PERIOD PER WEEK, 1989, DVPS............... 98 V-G-8

SUMMARY

OF MOLLUSKS (OTilER THAN Corbicula) AND DRAGOt1 FLIES COLLECTED IN IMPINGEMENT SURVEYS cot! DUCTED FOR Q1E 24-110UR PERIOD PER WEEK, 1989, DVPS............................... 100 V-H-1 HUMBERANDDENSITYOFFIgHEGGS,LAHVAE, JUVENILES, AND ADULTS (Number /100 m ) COLLECTED WIT 11 h 0.5 m PLANKTG1 NFT AT THE ENTRAINMD1T RIVER TRAMSECT IN Tite 01110 RIVER NEAR BVPS, 1989................... 105 V-I-l Corbicula COLLECTED IN UNIT 1 COOLING TOWER SEPTEMBER 7, 1989, DVPS................................... 119 V-I-2 Corbicula COLLECTED IN UNIT 2 COOLING TOWER MARCH 21, 1989, BVPS...................................... 122 V-I-3 Corbicula COLLECTED IN THE OHIO RIVER MAY 23, 1989, LVPS........................................ 124 V-I-4 Corbicula COLLECTED IN THE 01110 RIVER SEPTEMBER 14, 1989, BVPS.................................. 125 2 V-I-5 Corbicula DENSITIES (Clams /m ) SUMMARIZED FROM BENTHIC MACROItNERTEBRATE COLLECTIONS 1973 T!! ROU G il 19 8 9, hvP S........................................ ?26 viii

me LIST OF TABLES (Continued) TABLE Yage 3 V-!-6 Corbicula DENSITIES (Clams /100m ) PRESENT IN ICHTHYOPLANKTON SAMPLES COLLECTEE WITH A 0.5 m PLANKTON NET IN THE OHIO RIVER 1988 AND 1939, BVPS....................................... 128 V-1-7

SUMMARY

OF Corbicula COLLECT.- DURING IMPINGEMENT SURVEYS FOR ONE 24-HOUR PERIOD PER WEEK, 1989, DVPS............... 129 V-I-B RESULTS 00 - cula GROWTH STUDY IN INTAKE STRUCTURV

• UNIT 1 CCOLING TOWEP, 1989, DVPS............

133 V-I-9 RESULTS CF THE Corbicula LhRVAE ST 'DY IN THE INTAKE STRUCTURE AND UNITS 1 AND 2 COOLING TOWERS, 1989, BVPS................................................ 139 ' 'l-10 RESULTS OF THE Corbicula LARVAE STUDY SIZE DISTRIBUTION IN THE INTAKE STRUCTURE AND UNITS 1 AND 2 COOLING TOWERS, 1989, BVPS................................................ 140 9 ix ___s_

DUQUESNE LIGl!T COMPANY 1989 ANNUAL DNIROl4H.:NTAL REPORT 3. INTRODUCTION This report presents a summary of the Non-Radiological Environmer.t:3 Program conducted by Duquesne Light Company (DLC) dw ang calendar y y 1989, for the Beaver Valley Power Station (DVPS ) Units 1 and 2, Operatwg License Numbers DPR-66 and HPF-73. This is primarily an optionnl pro-gram, since the Nuclear Regulatory Commission (NRC) on February 26, 1980, granted DLC's request to delete all of the aquatic monitoring program, with the exception of fish impingement (Amendment No. 25), from the Environmental Technical Specifications (ETS), and in 1983, dropped the fish impingement studies from the ETS program cf required sampling along with non-radiological water quality requi: cments. However, in the inter-est of providing a non-disruptive data base DLC is continuing the Aquatic Monitoring Studies. A. SCOPE AND OBJECTIVES OF Tile PROGRAM The objectives of the 1989 environmental program were: (1) to assess the possible environmental impact of plant operation (including impingement and entrainment) ca the benthos, fish, and ichthyoplankton communities in the Ohio River, (2) to provide a sampling program for establishing a continuing data

base, (3) to evaluate the presence of Corbicula at the BVPS and to assess the population of Corbicula in the Ohio River, and (4) to study the growth and reproduction of Corbicula in the intake structure and cooling towers of BVPS.

9 l 1 i_-_____ _ _ _ _ _ _._.

m. DUQUESNE LIGIIT COMPANY 1989 ANNUAL ENV8RONMENTAL REPORT B. SITE DESCRIPTIO!! BVPS is located on the south bank of the Ohio River in t.he Borough of Shippingport, Beaver County, Pennsylvania, on a 501 acre tract of land. The Shippingport Station once shared the cite with BVPS before being decommissioned. Figure I-1 shows an aerial view of DVPS. The site is approximately 1 mile (1.6 km) from Midland, Pennsylvania 5 miles (8 km) from' East Liverpool, Ohio; m..: 25 miles (40 km) from Pittsburgh, Pennsylvania. Figure 1-2 shows the site location in relation to the principal population centers. Population density in the immediate ] vicinity of the site is relatively low. The population within a 5 mile 1 (8 km) radius of the plant is approximately 18,000 and the only area of concentrated population is the Borough of Midland, Pennsylvania, which has a population of approximately 4,000. The site lies along the Ohio Riv7r in a valley ~ hich has a gradual slope w extending from the river (elevation 665 ft. (203 m) above sea level) to an elevation of 1,160 f t. (354 m) along a ridge south of BVPS. Plant entrance elevation at the station is approximately 735 f t. (224 in) above sea level. The station is cituated on the Ohio River at river mile 34.8, at a loca-tion on the New Cumberland Pool that is 3.3 river miles (5.3 km) down-stream from Montgomery Lock and Dam and 19.4 miles - (31.2 km) upstream from New CA. erland Lock and Dam. The Pennsylvania-Ohio-West Virp'.14 border is 5.2 river miles (8.4 km) downstream from the site. The ri.C flow is regulated by a series of dams an3 reservoirs on the L> v ru, Allegheny, Monongahela, and Ohio Rivers and their tributaries. Flow gen-erally varies from 5,000 to 100,000 cubic feet per second (cfs). The - range of flows in IM9 is shown on Figure I-3 as well as Table I-1. U Ohio River wa te r temperatures generally vary from 32 to 82 F (0 to 0 28 C). Minimum and maximum temperatures generally occur in January and July / August, respectivelv. During 1989, minimum temperatures were observed in December / January and maximum temperatures in August (Figures I-3 and Table I-1). 2

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-.. ~. - =. ... ~. -. -. DUQUESNE LIGHT COMPANY _*~ 1989 ANNUAL ENVIRONMENTAL REPORT I y [p g OkyR M* ...... t e y., d 9-l I ,1 / e 'h,,,,,,,,, i ' -.- /,' I ",d%, b) h mj y. __ 9 _ R pk f) ~N ~ m i en t S a s ' / s \\ o j. WOtANO = X, Q Q .,o. WAvlR VAll!Y.

• FOWER SI A110N

, ~ ~. ~ ' /, g 'Nf,s .,b d)js 6-ma i. nii 1 FIGURE I-2 LOCATION OF STUDY AREA, BEAVER VALLEY POWER STATION SHIPPINGPORT, PENNSYLVANIA BVPS 4

DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT FLOW (cfe - X 1000) 200 ~A-176 150 125 / 100 g 75'# \\\\ 7N Au x 25 y -v y E 0 JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC TEMPERATURE (oP) 100 80 - ^ -so / / 20 l f 0 JAN FEB MAR APR MAY JUN JUL AUG SEP OCT NOV DEC 7 MONTH j MONTHLY MIN -+- MONTHLY AVE -G-MONTHLY MAX FIGURE I-3 OHIO RIVER FLOW (cfs) AND TEMPERATURE (O } p RECORDED BY THE U.S. ARMY CORPS OF ENGINEERS l FOR THE NEW CUMBERLAND POOL, 1989 l DVPS 5 l

TABLE I-l OHIO RIVER FIDf (cfs) AND TEMPERATURE (OF) RECORDED BY THE U.S. ARMY CORPS OF ENGINEERS FOR THE NEW CUMBERLAND POOL,1989, BVPS Jan Feb Mar Apr May Jun Jul Aug M Or:t Nov Dec w 3 Flow (cfs x 10 ) g e Monthly Maximum 91.5 146.0 183.0 177.0 183.0 198.0 73.0 36.0 34.0 66.0 67.0 55.0 $8 5O Monthly Average 46.1 59.9 63.8 66.2 07.3 81.4 33.1 15.5 16.4 24.6 34.3 22.3 E@z GM Monthly Minimum 20.0 13.0 27.5 20.0 31.0 34.5 16.0 7.0 8.5 12.0 12.5 7.5 ~$C = ge Temeerature (OF) k g3 o h Monthly Maximum 39 37 4;: 52 63 70 78 80 79 69 57 41 et Monthly Average 36 36 41 47 56 67 74 78 75 62 50 34 E ~6 Monthly Minimum 34 34 34 42 51 62 70 76 70 56 43 30

m DUQUESNE LIGl!T COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT i BVPS Units 1 and 2 have a thermal rating of 2,660 megawatts (Mw). Units 1 and 2 have a electrical rating of 835 Mw and 836 Mw, respectively. The circulating water systems are a closed cycle system using a cooling tower to minimize heat released to the Ohio River. Commercial operation of DVPS Unit 1 began in 1976 and Unit 2 began in 1987.

e.,,

v; .i E: e e e 7

4 DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT II.

SUMMARY

AND CONCLUSIONS The 1989 BVPS Units 1 and 2 Non-Radiological Environmental Monitoring Program included surveillance and field sampling of Ohio River aquatic life. This is the fourteenth year of operational monitoring for Unit 1 and the second for Unit 2 and, as in the previous operational monitoring years, no evidence of adverse environmental impact to the aquatic life in the Ohio River near BVPS was observed. The Aquatic Environmental Monitoring Program included studies oft benthos, fish, ichthyoplankton, impingement, plankton entrainment, and Corbiculs. Sampling was conducted for benthos and fish upstream and downstream of the plant during 1989 to assess potential impacts of ' BVPS discharges. These data were also compared to preoperational and other operational data to assess long term trends. Impingement and entrsinment data were examined to determine the impact of withdrawing river water for in plant use. Corbiculaf studies were initiated to determine the presence of these clams in the Ohio River and their growth and reproduction inside the plant. The following paragraphs summarize these findings: BENTHOS. Substrate was probably the most important factor controlling - the distribution and abundance of the benthic macroinvertebrates in the Ohio River near BVPS. Sott muck-type substrates along the shoreline were conducive to worm and midge proliferation, while limiting macroinverte-brates which require a more stable bottom. At the shoreline stations, Oligochaeta accounted for 89% of the macrobenthos collected, whereas Chironomidae and Mollusca each accounted for about 6% and 31, respec-tively. Community structure has changed little since preoperational years and there was no evidence that BVPS operations were affecting the benthic community of the Ohio River. I I l 8

em DUQUESNE LIGHT COMPANY j 1989 ANNUAL ENVIRONMENTAL REPORT PHYTOPLANKTON. The phytoplankton community of the Ohio River near BVPS during 1989, exhibited a seasonal pattern similar to that observed in previous years. This pattern is common to tempercte, lotic environ-ments. Total cell densities and diversity indices were within the range observed during previous years. Diatoms, green algae, and microflagel-lates were the most abundant groups in the phytoplankton during 1989. OOPLANKTON. ';ooplankton densities throughout 1989 were typical of the temperate zooplankton community found in large river habitats. Popu-lations developed the highest densities in September. Except during August, protozoans and rotifers were always predominant. Common and abundant taxa observed in 1989 were similar to those reported during preoperational and other operational years. Based on the data collected during the fourteen operating years (1976 through 1989) and the three preoperational years (1973 through 1975), it is concluded that the over-all abundance and species composition of the zooplankton in the Oh>) River near BVPS has remained stable and possibly improved slightly over the seventeen year period from 1973 to 1989. Increased turbidity and current from high water conditions have the strongest effects of delaying the population peaks and temporarily decreasing total zooplankton iensi-ties in the Ohio River nt.c BVPS. FISH. The fish community of the Ohio River in the vicinity of BVPS has been sampled from 1970 to present, using several types of gears electro-fishing, gill netting, and periodically, minnow traps and seines. The results of these fish surveys show normal community structure based on apt.cies composition and relative abundance. In all the surveys since 1970, forage species were collected in the highest numbers. This indi-cates a normal fish community, since game species (predators) rely on this forage base for their survival. Variations in total annual catch are attributable primarily to fluctuations in the population size of the forage species. Forage species with high reproductive potentials fre- ~ quently respond to changes in natural environmental f actors (competition, food availability, cover, and water quality) with large changes in popu-lation size. These fluctuations are naturally occurring and take place in the vicinity of BVPS. 9

a DUQUESNE LIGi!T C'J11PANY 1989 ANNUAL ENVIROt1MD4TAL REPORT Although variation in total catch has occurred, species composition has remained f airly stable. Since the initiation of studies in 1970, forage fish have dominated the catches. Carp, channel catfish, smallmouth and spotted bass, and walleye have all remained common species. Since 1978, sauger have become a common game species near BVPS. Dif ferences in the 1989 electrofishing and gill not catches, between the Contrcl and Non-Control Transects wer imilar to previous years (both operational and preoperational) JoLahly c,aused by habitat preferences of individual species preference is probably the most influential factor that af.= different species of fish are collected and in what relatis In the vicinity Data collected from 1970 thraugh 1989 indic: of the power plant have not been adversely affected by uVPS operation. ICHTHYOPLANKTON. Gizzard shad, and freshwater drum doininated the 1929 ichthyoplankton catch from the back channel of Phillis Island. Peak densities occurred in July and consisted mostly of eggs. No spawning was noted in April. The acnth of May showed little spawnirg activity. There was a decrease in larvae density af ter July. FISH IMPINGEMENT. The results of the 1989 impingement surveys indicate that withdrawal of river water at the BVPS intake for cooling purposes has very little ef f ect on the fish populations. Five hundred and fifty (550) fishes were collected, which was the fourth highest annual collee - tion s*nce initial operation of BVPS in 1976. Gizzard shad were the most numerous fish, comprising 79.5% of the total annual catch. The total weight of all fishes collected in 1989 was 4.05 kg (8.9 lbs). Of the 's50 fishes collected, 41 (7.5%) were alive and returned via the diacharge pipe to the Ohio River. PLANKTON D1TRAINMENT. Entrainment studies were performed to investigate the impact on the plankton community by withdrawing river water for in-plant use. Entrainment-river transect surveys for ichthyoplankton were conducted to ascertain any changes in spawning activity occurring in the 10

m DUQUESNE LIGHT COMPANY 1989 ANNUAL D4VIRONMD4TAL REPORT Ohio P.iver adjacent to the BVPS intake. The greatest abundance of ichthyoplankton collected occurred during the month of July. Assuming actual entrainment rates were similar to those found in 1976 through 1979, and adjusting for the water withdrawn for Unit 2 no substantial entrainment losses should have occurred in 1989 due to the operation of DVPS. Assessmer.t of monthly phytoplankton and zooplankton data of past years indicated that under conditions of minimum low rivet flow, approxi-mately 5% of the phytoplankton and zooplankton passing the intake would be withdrawn by the BVPS circulating water system. This is considered to be a negligible loss of phytoplankton and zooplankton relative to the river populations. Corbicula MONITORING PROGRAM. The results of the 1989 Corbicula Moni-toring Program show that no live clams were collected from the upper reservoir of Unit 1 cooling tower. Since the water entering this area comes directly from the condensers, it is suspected that elevated water temperatures make this area unsuitable for the clams. The Corbicula population in the lower reservoir was estimated at 300 million (90% alive) at the time of the September 7 sarvey. The estimated population of Corbicula in the Unit 2 reservoir on March 21 was 18.5 million (424 alive). The river surveys conducted in 1989 demonstrate that Corbicula inhabiting the upper Ohio drainage provides an extremely large number of clams to the DVPS. Cleaning of the intake bays by -divers resulted in removing many live clams f rom the innerbays: this along with the weekly impinge-ment data show that adult clams move into the plant with the water cur-rents. The results of the growth study obtained show that growth of Corbicula was initially more rapid in the cooling tower than in the intake struc-ture. Growth was more uniform for clams held in the cooling tower. The period of potential larvae release f rom gravid adult clams occurred in late June through September 1989 in the intake structure. Unit 1 cooling tower had a larval release period in the month of August, 11

DUQUESNE LIGiff COMPANY 1989 ANNUAL DIVIRONMENTAL REPORT Results of the larval cage study for the intake structure indicated that spawning activity in the Ohio River occurred from August through December of 1989. Chlorination of the cooling tower water may be a factor in the larvae study results. 9 = l 12

as DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT II1. ANALYSIS OF SIGNIFICANT ENVIRONMENTAL CHANGE The BVPS Unit 1 ETS, Appendix B to Operating License No. DPR-66, initi-ally required that significant environmental change analyses be performed on benthos, phytoplankton, and zooplankton data. However, on February 26, 1980, the NRC granted DLC a request to delete all of the Aquatic Monitoring Program, with the 0::ct; a of fiah impingement, f rom the ETS (Amendment No. 25, License No. DPR-66). Consequently, the requirements for Analysis of Significant Environmental Change was deleted by the NRC, and is not applicable to the present Aquatic Monitoring Program. In

1983, the NRC also deleted the requirem'nt for fish impingement studies.

However, in the interest of providing a non-disruptive data base DLC is continuing the Aquatic Monitoring Program. e l l l 13

DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT IV. MONITORING NON-RADIOLOGICAL EFFLUENTS A. MONITORING CHEMICAL EFFLUENTS The Environmental Technical Specifications (ETS) that were developed and included.as part of. the licensing agreement for the DVPS, required that certain non-radiological enemicals and the temperature of the discharges be monitored and if limits were exceeded they had to be repor ted to the NRC. During 1983, the NRC (Amendment No. 64, License No. DPR-66) deleted these water quality requirements. The basis for 'chis deletion is that the reporting requirements would be administered under the NPDES per-mit. However, the NRC requested that if any NPDES permit requirements were exceeded, that a copy of the violation be forwarded to the Director, Office of Nuclear Reactor Regulation. B. HERBICIDES Monitoring and reporting of herbicides used for weed control during 1989, is no longer required as stated in Amendment No. 64; thus, this informa-tion is not included in this report. -4 14

O . DUQUESNE LIGHT COMPANY ^ 1989 ANNUAL ENVIRONMENTAL REPORT V. ' AQUATIC biONITORING PROGRAM A. -INTRODUCTION The environmental study area established to assess potential impacts consisted J of ' three ~ sampling - transects _ (Figure V-A-1). Transect 1 is located-at river mile (RM) ' 34. 5, approximat.ely 0.3 mi (0.5 km) upstream of'BVPS and is the' Control Transect. Transect 2 is located approa:imately 0.5 mi (0.8 km) downstream of the BVPS discharge structure. Transect 2 is divided by-Phillis Island; the main channel is designated Transect 2A and the back channel Transect 2B. Transect 2B is the tr incipal - Non-Control Transect - because the majerity of - aqueous di::Garges from BVPS Unit 1 and 2~ are released to the back channel. Transect 3 is located approximately 2 mi (3.2 km) downstream of BVPS. Sampling dates' for each _ of the program elements are presented in Table ~ .V-A-1. The _ following sections of this report present a summary of findings for each-of the program elements. ~ B.- BENTHOS Objectives The. objectives of the benthic surveys were to characterize the benthos of - the Ohio. River near BVPS and to determine._ the innpacts, if any, of BVPS ~ I operations. Methods Benthic surveys were performed in May and September, 1989. Benthos samples were -collected at Transects _1, 2A, 2B, and 3 - (Figure - V-B-1), using a Ponar grab sampler. Duplicate samples were taken of f the south shore at Transects 1, 2A, and 3. Sampling at Transect 2B, in ~ the back channel of Phillis Island, consisted of a single Ponar grab at the south, middle and-north side of the channel. 15

s- ,-s L% v =.a p, gewu.. (K+L,r9jiE'; % - i yi4 3_ . t:- e.mg:_.=ac2h %?:sMIDLAND h$$Ei TRANSECT 3 'TVs@$i$$*D' ( 4e e

• UWyWD N

7 pm , g* ,m , 4, , s. ruar w<f 'W%ca'fM M 9 ...e 3 go ,/ h C TRA tJSE CT I "E l e \\4 nn I TRANSECT 2A qe 4 w"=** \\ -D as 't -n m =4 - i 4 t LEGEND k h hO e :.i '~ Q ~ 1 O D1 BEAVER VALLET DIS 3AltGZ j 'B g.$ ~ D2 INDUSTRIAL DIS 3ARGE BEAVER c e AID to sAvIaA :s InnsurssIm :.Ixz TRANSEC4 2 VAI.:.rv POWER O STATION e i FIGURE V-A-1 SAMPLING TRANSECTS IN THE VICINITY OF THE BEAVER VALLEY POWER STATION t BVPS i i

n... 1 '4 TABLE V-A-1 AQUATIC MONITORING P10 GRAM SAMPLING DATES 1989 BVPS 'Corbicula-I"I -Ichthvoplankton Phyto..and Month Benthos Monitoring Fish ' Impingement g-Night. Zooplankton January 13, 27 6,.13, 20, 27.- '13. February ,10,'17, 24 3,.10 24 . l:.. i y - w March 10, 17, 20, 21,'22-3, 30, 17,-24 17 ' ' . E -[ - g g., April-7, 14, 21-7, 14,.21, 28,- 13 14 zo ' CC-g tu - May 23 5,-12, 19, 23 23, 24 5, 12 23 24 19 E Ea June 2, 16, 30 2, 9, 16, 23, 30 19 16 . $[ -f U 12 13 13 g$ j July 13, 28 12,.13 7, 14, 21, 28 80 ' gn -gk [ August 11, 18, 25 4, 11, 18, 25 15 18 r- > E September 14 6, 7, 8, 14, 15,- 18, 19' 1,.8, 15, 22, 29 15 - g 22 .o. E-1 October 6,:13, 20 6, 13, 20, 27 13-s November .2, 10, 17 6, 7 24 17 r December 1,'15, 29-29 15' } (a) Corbicula Monitoring also includes all Impingement dates. i .g 'ph Jr v,

l I I t N N ~N ~. - s . mm. -.a --s e.kJ E ".. j '21* a?I?. j.;agg:._ ?NhkhTE$t Q>,t (#egp ?i % s, h +, WWMIDLAND -- waewswed. ( w i.mpbec,e-im e 3 a-i '%n. - sr r A ?- N g 1 '5 wee. p. em. pacgg nucz N ausnzw wwwa ~ 4, n.wr \\ ,m:vm:n e w w.:v e ?&. N %s's.iW-: 4 -a,=,n, -y.. 5i ggy ,. \\..,. n mJ /...,., c e

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4 a

TRANNION I.INE VALLET ('reeeneem yy O STATION e FIGURE V-B-1 l BENTBOS SAMPLING STATIONS BVPS

DUQUESNE LIGHT COMPAN. 1989 ANNUAL ENVIRONMENTAL REPORT Each grab was washed within a U.S. Standard No. 30 sieve and the remains placed in a bottle and preserved with 104 formalin. In the laboratory, macroinvertebrates ware sorted from each sample, identified to the lowest 2 posaible taxon and counted. Mean densities (numbers /m ) for each taxon were calculated for each of two replicates and three back channel sam-ples. Three species diversity indices were calculated: Shannon-Weiner, evenness indices (Pielou 1969), and the number of species (taxa). Habitats Substrate type was an important f actor in determining the composition of the benthic community. Two distinct benthic habitats exist in the Ohio River near BVPS. These habitats are the result of damming, channeliza-tion, and river traffic. Shoreline habitats were generally soft muck substrates composed of sand, silt, and detritus. An exception occurs along the north shoreline of Phillis Island at Transect 2A where clay and sand predominate. The other distinct habitat, hard substrate, is located at midriver. The hard substrate may have been initially caused by chan-nelization and scouring by river currents and turbulence from commercial boat traffic. ?if ty macroinvertebrate taxa were identified during the 1989 Monitoring Program (Table V-B-1). Species composition during 1989 was similar to that observed during previous preoperational (1973 thrcugh 1975) and operational (1976 through 1988) years. However, three new taxa (Nais behningi, Pristina longisoma, and Asellus sp.) were encountered during 1989. The macroinvertebrate assemblage during 1989 was composed primar-ily of burrowing organisms typical of soft unconsolidated substr a te s. Oligochaetes (worms) and chironomid (midge) larvae were abundant (Tables V-B-2, V-B-3, and V-B-4). Common genera of oligochaetes were Limnodrilus, Nais, and Paranais. Common genera of chironomids were Cr yptochironomus, Polypedilum, and Chironomus. The Asiatic clam (Corbicula), was collected f rom 1974 through 1978, and 1981 through 1989 surveys. None were collected during 1979 or 1980 surveys. 19 1

8 TABLE V-B-1 SYSTDtATIC LIST Of MACRCINVERTEBRATES CDi.LECTED IN Pf20PERATIONAL AND OPERATIONAL TEARS-IN THE OHIO RIVER NEAR BVPS Operational Preoperational 1973 1974 1975 1976 1977 1978 1979 1980 1981 1982 1983 1984 1985 1986 1987 1988 1989 Porifera X spongilla fragilis Cnidaria Hydrozoa Clavidae l Cordylophora lacustris X X X X l Bydridae I Craspedacusta sowerbyl X X X X X X X X X Hydra sp. H I l Platyhelrainthes e X I X X X Tricladida X ty X X X Rhabdocoela ZC X X X X X X CCh$ Nemettea X X X X X Y X X X X X X X X X Nematoda = k Urnatella gracilis X X X X X X X X X X X X X X X X $[ Entoprocta 89 O X ~q E l Ectoprocta X X X X l Federicella sp. $g % @p X X Paludicella articulat.a X Pectinatella sp. Fg X Plumatella sp. m Annelida o Oligo:haeta y X X X X Aeolosomatidae X X X X X X X X X X X Echytr aeidae Naididae X Atphichaeta leydigil X X Amphachaeta sp. X X X X X Arcteonats lomondi X X Aulophorus sp. X X X X X X Chaetogaster diaphanus X X X C_. damstrophus X X X Dero digitata D. nivea I X X X X X X X X X X X X X Dero sp. X X Nais barbata X N. behningi N. bretscheri X X X X X X X N. communis X X X X X X X X X X X N. elinquis X X N. simplex

m. .m. t TABLE V-S-1-- .(Continued) Preoperational _ opera tional 1973 1974 1975 1976 1977 1978 1979 1980 1991 1982 1983 1994 1985 1906 1987 199e 1969 .l N. variabilis I X X X ' it-is sp. X X-X X X X X X X X X I' X X X X l Ophidonais serpentina _X X X. 'X X' X. ] Paranais frici .X I X X X X -- X X X' X X X X X I X j Paranais sp. X j Pristina longisoes X

t. werni X'

X X X X { .f. sina .X X X X X X Pristina sp. X X ' Slavina appendiculata X Stephensoniana trivandrana X X X. X X X ' Stylaria fossularis X S. lacustris-X X X 'X p ' theinais useinsta X V_ejdovskyella intermedie X X X X w Veidovskyella sp. X T2Lilicidae 5@ 'p g - Aulodrilus liartobius I X X X X X-X X X X X X + A. pigueti I X X-X X X' X X X X X X X 2- [.pluriseta'_ X X X X X X X X X X g to Berthrioneurs vejdovskyanum 'I I X X X I < t*. ' [ "b' j Branchiura sowerbyi X' X X X X X X X X X X X X X X Ilyodrilus templetoni' X X X X X X X X X X X I Llanodrilus cervix X X X X X X X X X X-X X X X X $EO L. cervix (variant) X X X X X X X X X j L. cleparedeianus .X.. X X X X X X X X X X X X X X

• i h :>.

L. hoffmeisteri X X X X' X X X. X X X 'I X X X X X 'I L. spiralis I-X X h L. udetenstanus X' X X X X X X X X X X X X X X X X l Limnodrilus sp. X Peloscolex multisetosus longioentus 1 I X X g P,. m. multisetosus X X X X X X X X X X X X Potamothrix soldaviensis 'X X X P. ve3 doe 8]Ii_ X X X X X X Pan - ryctides curvisetosos 1 [ Tubtfex tubifex X X X X X X l- [ Unidentified immature forms ' with hair cbsetae X "X X 'X X X X X X X X X X X X X X i 2 without hair chnetae X X X X X X X X X X X X X X X X X Lumbeiculidae Birudinea Glossiphoniidae X .t Belobdella elongata X X ,t B. stagnalis X Beloedella sp. I j Erpobdellidae [ i ' Erpobdella sp. X l ) Moorecedalla microstoma X X g s- +

a - 4 4 b TABLE V-D-1 (Cerktinued) Prooperational' operational 1973 1974 1975 1976 1977 1978_ 1979 1980 1981 1982 1983 1984 1985 1986 1987 1988 1989 'Arthropode Acar in a. 1 X .I-X X X Ostracoda X 'X' X Isopoda Asellus sp. X Aghipoda Talitridae Byallela azteca - X X f:ammaridme Crangonyx pseudogracills X Crangonyx sp. 'X Gammarus fasciatus 'I X 1 7 5 c anmarus sp. 'I. X X X X X X X X X X X X 'X [ Decapoda.. I Coileabo11a X Epneseroptera g rg i Beptag enildme X X r m Stenacron sp. X X $G a. Stenonetas sp. X gM r Ephemeridae <g { g Bexagenia sp. X X X X X

• "* y 5=

l DJ Baetidae X Caenidae gd Caenis sp. X X X gO ' Tricorythodes sp. X w'3 E- - mae Ephesera sp. X Megloptera. Sialis sp. I m h ta o

xy Gomphidae

• 3 Drmogosphus spoliatus, X

Dromopomphus sp. X I l mwNs q. X X X X X l Libe11ulidae l Libellula sp. I Trichoptera Hydropsychidae. X-Oteumatopsyche op. X X Bydropsyche sp.. X Bydrop:111dae Hydrootila sp. X [ Orrethira sp. 'X [ Leptoceridee Oecetis sp. X X X X X X X. Polycentropodidae Polycentropus sp. X X t I

i TABLE V-a s. (Continued) Preoperatlona1 ..1976 1977 1978 1979 1980 1981 1992 1983 1984 1985 1986 1987 1988 19e9 Operations 1 1973 1974 1975 Coleoptera X. X Hydrophilidae X X1.aidae Ancyronyx variegstus X l Dubiraphia sp. X X X Belichus sp. X btenelmis sp. X , 1- .X Psephenidae Diptera Unidentified Diptera X X.- X X X X X 'X X .X Psychadidae X Perleoma sp. X Psychoda sp. 1 Telmatoscopus,sp. X M Unidetatified Psychodidae pupae X Chaoboridae 'O Chaoborus sp. X X X X X X X Simulidae gg EO Simillum sp. X cc. t Chirortomidae hE Chironominee X X Z Chironominee pupa X X X X X X X Chironomus sp. X X -X X X X X X X X X X X X X MC sa Cladopelma sp. X X X g0 Cryptochironomus sp. X X X X X X X X X X X X X X X X X y Dicrotendipes nervoeus X $g Dierotendipes sp. .X X X X X X dg Eyptotendipes sp. X X X g l Barnischia sp. X X-X X X X X X X X' X X X g' g4 Micropsectra sp. X Microtendipes sp. X 9 [ Parachironomus sp. X X O Polypedilum (s.s.) convictum type X P. (s.s.) simulans type-X Polypedilum sp. X X X X X X X X X X X Rheotanytarsus sp. X X X X X X X X X i Stenochironomus sp. X X X X Stietochironomus sp. X Tanytarsus sp. X X X X X X X Xenochironomus sp. X [ Tanypodinae. l Tanypodinee' y e X X I Ablabesmyia sp. X X X X Coelotanypus seapularis 1 X X X X X X X X X X X X l Djalmabatista pulcher X .{ Procladius (Procladius). X X X [ Procladius sp. X X X X X X X X X X X X X X X X X. Thienemannisyle group X X X X X f 2avre11myia sp. X i l e a n.

4 a ,e a TABLE T-s (Continued) ' Preoperational Oper a tional 1973 1974 1975 19 76 19 7 7 1978 19 79 1990 1981 198 2 1$ 43 1934 1985 1986 1987 190s J9ep Orthocladiinse '2'. 'Orthocladiinae popee I Cricotopos bleinetas I C,. es.s.2 tritascia 2 Cricotopos (1socladius) sylvestris Greap 2 [ C. (loocladies) sp. 1 [ 1 'Cricotopos ts.s.) sp.' .I.. 2 I X X. I I .1 1 1 Eutiefferiella sp. eydreceenus sp. I Limnophyes sp. 2 L Mannocladica (s.s.) distinctes I I I 2 X Nannocladius sp. I X Orthoctedius sp. I 1 X X X X 'X 1 2 x Parametrtoeneinua sp. 2 X Parapneenociadnes sp. 'I I y. Psectrocladsus sp. 1 X. Pseudorthocladius sp. I Pseudoenittas sp. I 2

k {

Sn2ttia sp. I I 2 1 1 e,a - s,nse zo Diamesa sp. I cc p Pottnastia sp. 2-ge m Ceratopogenidae E 2 I I. I X X I 2-I x Z $M' colichopodidae 2 2 $.,y {n Depididae I I I 2 7 M ' Wiedemannia sp. 2 Ephydridae I b= hd . Muscidae 2 2 Rhagionidae I QO Tipulidse 2 x y Str atiomyiidae I 2 x >U> Syrphidae 3 g g *< [ Lepidoptera X X X felluses y Gastropoda O Ancy11dae y. [ Perrissia sp. 2 I I I ^ 4 Planorbadae X valuatidae valvata perdepressa Pelecypoda 2 Coebicalidae Corbicula man 11ensis* I 2 2 2 2 x z' x x x X X X 2 Spbaeridae; X X X Pisidium sp. 1 X X X Sphaertsen sp. I I I I I 2 1 I 2 I I 2 Unidentified immature Sphaeriidae; I 2 2 2 f Unionidae Anadonts grandis I-I 5 Elliptao sp. 2 { Coadentified immature tinionidae 1 2 I 2 1 'Recent literstat e relegated all North Asterican Corbiesla to be Corbienia fluminea. L 4 I

TAB 2 V-B-2 2 MEAN NUMBER OF MACROINVERTEBRATES (Number /m ) AIO PFJtCENT COMPOSITION OF OLIGOCHAETA, CHIRONOMIDAE, MOLLUSCA AND OTHER ORGANISMS, 1989 BVPS STATION 1 2A 2B 3 6/m 9/m g gj,2 g gj,2 g 2 2 w May 23 I w j. 011gochaeta 3,390 98 0 0 1,827 .78 3,471 93 E E-Chironomidae 49 1 40 57 238 10 228 6 E@ 4 Mollusca 0 0 0 0 39 2 10 <1 -E E Others 20 1 30 43 231 10 30 1 g 1l5 < t* ff '[ g m tals 3,459 100 70 100 2,3 35. 100 3,739 100 E* gn September 14 gk t- > 5 'l Oligochaeta 1,440 92 20 14 3,586' 85 2,198 91 g Chironomidae 90 6 -0 0 309 7 109 5

• e

.t Mollusca 20 1 118 80 224 5 108 4 0 Others 10 1 10 7 93 2 10 <1 L % tals 1,560 100 -148 101 4,212 99 2,425 100 .l [ .l 5 i i g i e a 5 e t

DUQUESNE LIGitT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT TABLE V-B ~J 2 BENTHIC MACROINVERTEBRATE DDISITIES (Number /m ), MEAN OF TRIPLICATE FOR BACK CllANNEL AND DUPLICATE SAMPLES COLLECTED IN THE MAIN CIUJ4NEL O!!IO RIVER, MAY 23, 1989 BVPS STATION Taxa 1 2A 2B 3 Nematoda 10 Ectoprocta unidentified 4 + Entopr oc ta Urna tella gracilis + Annelida Enchytraeidae 7 20 Oligochaeta eggs + + Arcteonais lomondi 10 10 Dero sp. 10 Nais behningl 59 Nais communis 20 125 30 Nais simplex 7 10 Nais variabilis 33 Ophidonais serpentina 13 .Paranais frici 89 33 798 Pristina Jongisoma 7 + Pristina sima 7 Vejdovskyella intermedla 20 7 128 Aulodrilus plurise ta 10 Branchiura sowerbyi 20 7 10 Limnodrilus cervix 30 7 40 Limnodrilus claparedianus 20 Limnodrilus hof fneisteri 640 144 355 - Limnodrilus udekemianus 144 98 Potamothrix vejdovskyi 26 20 Immatures w/o capilliform chaeta 2,108 1,149 1,124 Immatures w/ capilliform chaeta 433 59 228 Arthropoda Isopoda Asellus sp. 13 Amphipoda .Gammarus sp. 't0 197 Epheme r op tera Bae tidae la Hexagenia sp. 7 Diptera l Chironominae pupse 10 l Chironomus sp. 7 30 Cryptochironomus sp. 39 105 49 Dicrotendipes sp. 7 t 26 l

DUQUESt1E LIGi!T CCEPANY 1989 A!4HUAL DIVIlONMENTAL REPORT TABLE V-B-3 (Continued) STATION Taxa 1 27. 2B 3 Polypedilum sp. 10 92 89 Coelotanypus scapularis 20 Procladius sp, 7 40 Cricotopus sp. 20 7 orthocladius sp. 10 13 Tipulidae 7 Stratiomyidae 20 Mollusca corbicula flumines 39 10 Total 3,459 70 2,335 3,739 + Indicates organisms present. 27 __.m_m _.m

DUQUE6!1E 1.1Cl!T COMPAliY 1969 AlillUA!, DIVIl!O!4MDITAL rep 0}tT TABLE V-B-4 2 BEN 11810 MACR 31NVERTEDRATE DENSITIES (NJmber/m ), MEAN OF TRIPLICATE IVR bat? CilANNEL AND DUPLICATE !WMPLt3 COLLECTED IN Tile MA1H CilANNEL Q110 RIVER, SEPTfftDER 14, 1989 DVPS STATION Taxa _, _ 1_ 2A 2D 3 Cnidar ia Hydra sp. 7 Hematoda 13 10 Entoptocks grnatella cracilis 4 + Annelida Oligochaeta eggs 4 + Arcteonais lomond! 7 Nale c<sumurie 13 7 }! alt sp. Parannis Prici 7 Autodt)lus p1'ariseta 13 h anchiure aowerby_! 26 t.imnodrt13 grvix 20 10 33 =0 3 Limnoonilus hoffmeisteri 404 361 424 Limnodrilus ujekemiar.uT 20 207 Immatures w[~ spiiliform chneta 82fi 10 2, 9 15 1,450 Tema tu re s w/ 8111 tora chasta 16e 204 69 $rudineh clossiphonild 7 Ar t.hropoda laopoda Asellus sp. 13 Amphipoda Gasmarus sp. 10 20 I optern Unidentified Israature 10 Tricoptera Decetta sp. 7 folycentropus,op. 13 Diptera Chitonominae ppae 7 Cntrononu_a sp. 10 59 10 Cryptoehttimomus sp. 60 66 Hstnischia sp. 13 Polypedilus sp. 20 112 99 Coelotanypu,_a, acapultor is 39 s Procladius sp. 13 Ceratopogonidae 13 Mallusca corbicula fluminea 20 118 197 108 ~ Pisidiuns sp. 7 20 Sphaer,, lum, sp. Total 1,560 148 4,212 2,425 I Indicates organisima present. 28

m._ DUQUESNE LIctrr COMI ANY 1989 ANNUAb DNIRONMD4 tab REPORT Ho ecologically impor tant additions of species were encountered during 1989 nor were any threatened or endangered species collected. Community Structure and Spatial. Distribution 011gochaetes accounted for the highest percentage of the macroinver te-brates at all sampling stations In both May and September (Figure V-B-2). Denrity and species composition variatlons observed within the DVPS study area were due primarily to habitat dif f erences and the tendency of cer-tain types of macroinvertebrates (e.g., oligochaetes) to closter. Over-all, abundance and species composition throughout the study area were similar. In general, the density of macroinvertebrates during 1989 was lowest at Transect 2A and higher at Transects 1, 2B, and 3 where substrates near the shore were composed of sof t mud or various combinations of sand and silt. The lower abundance at Trt.nsect 2A was probably rel ated to sub-strate conditions (clay and sand) along the north shore of Phillis Island. Comparison of Control and Non-Control Stations, No adverse impact to the benthic community was observed during leB9. This conc 1.asion is based on a comparison of data collected at Transect 1 (Co')t r C 1) and 2B (Non-Cantrol) and on analyses of species composition and densities. Data indicate that oligochaetes were usually predominant throughouw the study area (Figure V-B-2). Most abundant taxa at Transects 1 and 2B in both May and September were immature tubtficids without capilliform chaetae (Tables V-D 3 and V-D-4). In May, common or abundant taxa at both stations were the oligochaetes I.imnodrilus hoffmeisteri, Paranais frici, and the midge c y tochironomus. In September, the oligochaete Limnodrilus hoffmeisteri, the midge Cy ptochironomus, and the clam Corbicula fluminea were the common organisms collected at both stations, i 29 ,r_

1 / S. ,G D. p____ E 40-u y 20 - b g / 7 5l 5 E8 h$ / V j PR PER Tl NA Y ARS PE AT ONAL EA S I I CHIRONOMIDAE IEEi ALL OTHER8 E OLIGOCH AETA FIGURE V-B-2 N fBE N PREOPERATIONAL AND PERATIONAL YEARS

DUQUESNE LIGHT COMPANY A989 ANNUAL DNIRONMENTAL REP 0ltT In May and Septen.ber 1999, a greater diversity of organisms were col-- lected at Hon-Control station 2B than at Control station 1 (Table V-D-5). This has occurred several times during past surveys. The mean number of taxa and Shannon-Weiner indices for the back channel were within the range of values observed for other stations in the study area. Differ-ences observed between Transect 1 (Control) and 2D (Non-Control) and between other stations could be related to differences in habitat. None of the dif ferences were attributed to DVPS operation. 4 Comparison of Preoperational and Operational Data Composition, percent occurrence and overall abundance of macroinverte-brates has changed little f rom preoperational years through the current study year. Oligochaetes have been the predominant macroinvertebrate in the community each year and they comprised approximately 09% of the indi-viduals collected in 1989 (Figure V-D-2). A similar oligochaete assem-blage has been reported each year. Chironomids and mollusks have composed most of the remaining fractions of the community each year. The poten-tial nuisance clam, Corbicula, increased in abundance from 1974 through 1976, but declined in number during 1977. Since 1981, Corbicula have been collected in the benthic surveys including 1989. Total macrcinvertebrtte densities for Transect 1 (Control) and 2B (Hon-Control) for each year since 1973 are presented in Table V-D-6. Mean densities of macroinvertebrates gradually increased from 1973 through 1976 (BVPS Unit 1 start-up) to 1983. In 1909, total densities were greater than those of recent years. These densities were similar to those observed in 1982 and 1983 and they are well within the range of preoperational and operational year data. Mean densities have frequently been higher in the back channel of Phillis Island (Non-Control 2B) when compared to densities at Transect 1 (Contr ol). In years such as 1986 (also 1984, 1983, 1981, 1980, 1979) when mean densities were lower at Transect 2B than at Transect 1 the dif ferences were negligible. These dif f erences could be related to substrate variability and randomness of sampiv grabs. Higher total densities of macroanvertebrates in the back channel (Transect 2B) when compared to Traasect I was probably due to the 31

DUQUESt1E 1.lCitT COHFiJ41 1989 Alit 1UAL D4/IE01U%ilhL REPORT TADLE V-D-5 MEAN DIVERSITY VALUES FOR DEldTt!!C MACH 01HVERTEBRATES COLLECTED 114 Tile O!!!O RIVER,1989 DVPS STATIOli 1 2A 2D 3 DATE: Mry 23 No. of Taxa 10 2 13 18 Shannon-Weiner Index 1.78 1.25 2.14 2.46 Evenness 0.53 0.96 0.62 0.60 DATES September 14 Fo. of Taxa 8 2 15 8 Shannon-Weiner Index 1.07 0.88 2.95 1.86 Evenness 0.64 0.65 0.78 0.60 32

f nBIE v-B-6 BENTHIC 6IN7ERTSRATE DE2tSITIES (Number /m ) FOR SRTIOtt 1 i (CQtTROL) AND STATION 2B (EON-COttTROL) DURING FREDPERATIONAL AND OPERATIONAL TEAPJ BVFS i Precoerotional 70ere Operational Yeere 1973 197. 1973 1976 1977 1979 19 7e 6990 1991 1 2B 1 23 1 2B 1 5 1 25 1 D 1 3 1 D 1 23 j January p a February 205 8 703 311 338 299 312 1.100 1.499 2.343 L 929 1.294 marca 425 457 DC -C CO april CC > tv I$ may 248 504

• 118 2.197 927 3.560 674 040 351 126 1.904 see 1.041 747 209 454 en June 3

40 557 Ett < t* w ww w July 653 119 421 419 MO O=5* a.,.se n 244 lo su 1.e17 1.124 est ns s,1 3.4n al

1. n.

1.1 3 su an septe. tee les s2 1.s23 ees 2.les 112 3g 5 35 i Det e r 256 339 t* g h, he 149 292 31s 263 75. 817 389 1.695 Its 931 346 1.543 312 806 1 December O 2F 4 l a aa 231 zu en su su en n1 1.ns n1 1.ses n1 1.sz, es? En 1.19e eis 1.197 64 4 I t l

TABLE Y-D-6 (2mitifwed) Wretiensa Years 1982 1983 1954 1985 1996 1987 1898 1993 2B 1 2B 1 23 1 2B 1 3 1 2B 1 2B 1 23 1 January wW i Febroery ee March DU ~C ZO April May 3,490 3,C26 3.590 1.314 2,741 6 21 2.254 367 sol 969 1,971 2,649 1,8 04 1,775 3,459 2,335 CC > t-U9 t; E ~ 3" $C E.E July .3 ~ September 2,956 3,364 4,172 4,213 1,341 826 1,c24 913 849 943 2,910 2,780 1,420 1,5 14 1.560 4.212 E August -48 >e rg October w *C G6 m eeer w +3 Decevabe r Mean 3,223 3,195 3,881 2.764 2,041 725 1,640 890 725 956 2,443 2,714 1,612 1,645 2,5 10 3,274

DUQUESNE LICirr COMPANY 1989 ANNUAL ENVIRONMENTAL REPoirr morphology of the river. Mud, silt, and slow current were predominant at Transect 2B creating conditions more f avorable for burrowing macroinver-tebrates in comparison to Transect 1, which has little protection from river currents and turbulence caused by commercial boat traf fic. Sumary and Conclusions Substrate was probably the most important factor controlling the dis-tribution and abundance of the benthic macroinvertebrates in the Ohio River near DVpS. Soft muck-type substrates along the shoreline were conducive to worm and midge proliferation, while limiting.nacroinverte-brates which require a more stable bottom. At the shoreline stations, 011gochaeta accounted for 89t of the macrobenthos collected, whereas Chironomidae and Mollusca each accounted for about 6% and 3% respec-

tively, Community structure has changed. tittle since preoperational years and there was no evidence that DVPS operations were affecting the benthic community of the Ohlo River.

C. PHYTOPLANKTON Objectives Plankton sampling was conducted to determine the condition of the phy-toplankton community of the Ohio River in the vicinity of the DVPS. Methods One entrainment sample was collected monthly. Each sample was a one-gallon sample taken frou below the skimmer wall f rom one operating intake bay. This one-gallon sample was preserved with Lugol's solution and was used for the analyses of both phytoplankton and zooplankton. I In the laboratory, a measured aliquot of the sample was settled in an inver ted microscope chamber. A minimum of 250 cells were identified and counted at 400X magnification. For each collection date, the volume of 35 ---y ,y 7,__._,,n... y p.v. p m w.y%, _. y

l l DUQUESNE LIGHT COMPA11Y 1989 ANNUAL DIVIRONMDITAL REPORT sample settled and examined was adjusted depending on cell density. A Hyrax diatom slide was also prepared monthly from each sample. This slide was examined at 1000X magnification to make positive identification of the diatoms. Denrities (cells /ml), Shannon-Weiner (log base 2), and evenness diversity indices (Pielou 1969), and richness index (Dahlberg and Odum 1970) were calculated for each monthly sample. Seasonal Distribution Total cell densities of phytoplankton from stations on the Ohio River and in the intake samples have been similar during the past years (DLC 1976-1980). Species composition has also been similar in entrainment samples and those f rom the Ohio River (DLC 1990). Therefore, samples collected from the intake bays should provide an ade-quate characterization of the phytoplankton community in the Ohio River. During 1989, the January through May samples had phytoplankton densities of 1,570 to 6,502 cells /ml (Table V-C-1 and Figure V-C-1). Total mean densities increased in June and peaked in July at 15,464 cells /ml. After July, densities displayed a general decreasing trend to a low of 2,760 cells /ml in December (Table V-C-1 and Figure V-C-1). A small peak of 5,956 cells /ml occured in October. Diatoms (Chr ysophy ta ), green algae (Chlor ophyta) and microflagellates were generally the most abundant groups of phytoplankton during 1989 (Table V-C-1 and Figure V-C-2). The relative abundance for the group Cyanophyta was high only in January, when two colonies accounted for 78% of the total cell numbers counted. Relative densities of Cryptophyta was highest in April (54) (Table V-C-1). Diver:ity indices for the phytoplankton during 1989 are presented in Table V-C-2. Shannon-Weiner indices ranged from 1.36 to 4.32, evenness values fron 0.29 to 0.81, and richness values f rom 2. 85 to 6.12. High ( 1 2.00) diversity values occurred in 9 of the 12 months. The lowest value for Shannon-Weiner Index occurred in January when two colonies of 36

l TABLE V-C-1~ i 1 MONTHLY PETTOPIANKTON GROUP DENSITIES (Number /ml) AND PERCENT COMPOSITION l FROM ENTRAINMENT SAMPLES,-1989 svPs. i~ 2 Jan Feb Mar- 'Apr May Jun Group f/ml n 9/ml-t 9/ml 4 4/ml t 8/ml 4 (/ml 4 Chlorophyta 39 1 222 21 50 3 204 -10 26 1 57 1 j i Chrysophyta. 321 5 947 60 369 18 850 40 402 22 1,607 35 i Cyanophyta' 5,071 78 28 2 '220 11 8 <1 23 1 0 0 Cryptophyta 5 <1 21 1 12 1 115 5 28 2 51 1 y l } Microflagellates-1,061 16 252.' 16 1,348 67 876 '41 1,311 73 2,815 62 e Other Groups 5 <1 0 0 0 0 68 3 14 1 6 <1 E 4 i 2f o E Total 6,502 100 1,570 100 1,999 100 2,121 99 1,804 100 4,535 99 i' E "a i z i i Jul Aug Sep Oct Nov Dec E Group t/ml 4 t/ml 4 t/ml 4 t/al 4 f/ml 4 f/ml 4 bU 52 Chlorophyta 2,148 14 2,051 '25 1,549 34 1,348 23 248 8 161 6 5" -- h l Chrysophyta 10,203 66 3,298 41 1,823 40 2,794 47 888 27 614 22 Cyanophyta 0 0 14 <1 0 0 67 1 0 0 12 <1 pg j Cryptophyta 501 ~ 3 23 <1 155 3 260 4 69 2 50 2 m Microflagellates .2,539 16 2,743 34 1,059 23 1,479 25 2,088 63 1,923 70 E h Other Groups 73 <1 9 <1 6 <1-8 <1 5 <1 0 0 +4 Total 15,464 99 8,138 100 4,592 100 5,956 100 3,293 100 2,760 100 g ~.

1lll e8C5R =* n@$E ~5 egC5 n Q G1? wUs" C 'N N n E D 9 8 \\ 9 pg V 1 O R 3, N C EV x I T R x %"fv~ E 6 E5R C 8 OD G 8 IN 9 HA 1 O P ) S b ^s S 7 H7A V 9 T9E s NY 1 1 G E e* h U I4) G 79 A A S98 R 1 E19 L E I(1 =* UH N C T IL6 JT V SA7S N NN9P / NO l E EO1V y/ UM U T R DI( B s g J G iAL r F WEN RA I Y S rPO c A R HOI 7 + M Y AET / LRA 'i L PPR R A O E s ~ J GP / P N nNO A A O il I R IT PR / ' A R YD R A U E V{ ' E T L M E H IL \\ P T I O N L x L \\ B E O nv A R M I M l F N x P v/ / K N S m-g ' A O L J L EC 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 1 1 lll l 11 ll' l(lllll f

4 ? I TABLE V-C-2 i PHY2DPLANKTON DIVERSITY INDICES BT MONTH FOR ENTRAIPMDIT SAMPLES,1989 BVPS t Jan Feb Mar Apr May _. Tun No. of Species 27 46 25 45 26 25 Shannon-Weiner Index 1.36 4.32 2.00 3.26 1.81 2.11 >c Evenne ss 0.29 0.78 0.43 0.60 0.38 0.45 5c EO l >n Richness 2.96 6.12 3.16 5.74 3.33 2.85 t' 5e Y SC g Jul Aug Sep Oct Nov Dec X g _S i 5" No. of Species 37 29 24 30 34 29 31 (2 o Shannon-Weiner Index 2.80 3.01 3.70 3.53 2.16 1.95 2.67 tt

o 4 i

n Evenness 0.54 0.62 0.81 0.72 0.42 0.40 0.54 g

e

• 3 i

Richness 3.71 3.11 2.85 3.34 4.07 3.53 3.73 ~ i g l

l!l11l11 1 l1l <l \\j 1 iji) { l! iI1i -{Il1 @o$znrE5 nR:DE

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• v G

S TO U Y PR ~ i R YC s, A ./ H RI us C CM zE / L oL / UH 2 P f ,.+ I i rM J T 0 C uA N oS / e / s /- NO V n s aTr s/* UM E Nv \\ \\ J R nEs .i U oM Y I eI A \\ R oE G rN \\ Y s A F nA i M uR x / LT rN = i P r m/ rR \\ A aO k \\ R R A s rF E '6 L. \\ T A T f--' i M A Y I L T -=bA ,UT . E P O N B H P Y H ILL I M F O R A \\ O \\ i N / L N AY H \\ n y-S \\ C i A C C LL J E C C7 ~ 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 1 0 1 1 l# l;4 1 sji i 1;] i j: i -

~. DU90ESNE LIGilT COMPANY 1989 ANNUAL DIVIRONMENTAL REPORT blue-green algae dominated the cel? counts; however, the lowest number of species occurred in September when flagellates and small centrics were most predominant, liighest number of u _ ;16) cr.; curred in February. Phytoplankton communities were generally dominated by different taxa each season. The nost abundant taxa during winter (January through April) were microllagellates and small centric diatoms (Table V-Ce3). Micro-flagellates continued to be most abundant in June and July. Skeletonema potamos a diatom was dominant in August. Microflagellates and small centrics were most abundant during fall and winter. Comparieon of Control and Non-Control Transects Plankton samples were not collected at any river stations af ter April 1, 1980, due to a reduction in the scope of the Aquatic Monitoring Program, therefore, comparison of data was not possible in 1989. Comparison of Preoperational and Operational Data The seasonal succession of phytoplankton varied trom year to year, but, in general, the phytoplankton taxa has remained consistent. Phytoplank-ton communities in running waters respond quickly to changes in water temperature, turbidity, nutrients,

velocity, and turbulence (Hynes 1970).

The phytoplankton from the Ohio River near DVPS generally exhib-ited a bimodal pattern of annual abundance. During the preoperational year 1974, total densities peaked in August and October, while in opera-tional years of 1976 through 1979, mean peak densities occurred in June and September (DLC 1980). Total phytoplankton densities also displayed a bimodal pattern in 1989, when peaks occurred in July and October. The increased density in January was not a true reflection of a phytoplankton peak because two blue-green colonies accounted for 78% of the total cell density. In general, the phytoplankton community in 1989 was similar to those of preoperational and operational years. No major change in species compo-sition or community structure was observed during 1989. The small dif-41 y --yr. g +y w-ww

..__u_m +- a Tha!J: v-C-3 DENSIT17lS (Number /al) OP FOST ABUIElngrr PETTOPIAIEtTON TANA (Fifteen feet Abundant On Any Date) CDL12CTE FMM ENTIrlietsr! SAMPLES i~ 1 aman? tem 00er DEsmER 1999 g f } ) j Tax Jan Feb' Mar Ag M Jon Jul A3 M M .M M l t i CYANOPETTA 4 s APhenotheca nidulans 4,862 4 Dec111steria forurtee 18 Schizothrin celeicola 14 21 : 23 12 Schitothrix spp. 1 Coccoid cyanophrts 177 199 4 w CHIDMOPETTA .e 4 w Ankistrosems -vivolutus 9 7 7 il 5 34 100 99 161 302 ?.1 44 j Antistro&omas falcatus 12 14 25 47 16 17 300 180 12 185 21 55 $8 l Chlorophyta 1 240 120 773 189 331 128 43 2o 45 g$.- [ } Coelastres microporum 25 9 9 l i Crue19enta tetrapedis Dici16miten pulebe11tse 73 126 149 23 gE [ Micrectinium poeillue 36 168 yp ) 74 [g Pediastrum borva-nus co Pediastrum duplex 127 6= i Seen-deanos ecumtinetusJ 73 50 67 18

*9 ho f

Scen*6eeses bleellularis 221 757 132 15 3 y@ Scenedessus denticulates 9 7 9 7 91 138 50 84 Scenehemos opolensis 36 68 > "o i seen*&esus truser teande - 9 14 ? 5 55 216 279 101 32 5 18 ?? 67 9 18 Seen*6eemus spinosos 4 149

• e i

Sce-.4 % __ ; spp. 3 I Sptwertrystis schroeteri 73 180 O I Ulott: rim enbt111stime 21 I t 4 I I Achnenth*9 minutissime 66 108 22 12 83 276 43 44 l Asterionelle fermee 23 Il 32 O 5 23 228 5 6 122 i Cyneelle ventrieone 28 11 9 29 8 5 5 Dietone tense 28 j 35 9 4 2 29 35 7 Distone volgere a j 2 Dinceryon becaricus 5 4 14 6 i rrectitoria c.peeine 22 I l Fre,11 erie crotonensis 18 218 94 l Frocilaris voucherice 28 5 11 Melosire ambitrua le 9 55 397 304 50 5 420 23 21 Melostre distans l Melostre granulata 9 22 51 73 45 211 1 34 5 seelostra vertans 9 70 5 14 9 29 23r 17 18 5 I

TABLE V-C-3 (Centinued) Taxa Jan Feb Mar Ar:r

Mnay, Jun Jul g

g Oct Iksv te Navicula cryptocephala 16 112 32 43 32 51 5 6 12 7 Navicula virieals 9 84 21 36 41 314 18 6 30 12 Nitrachia acteularia 4 5 34 9 8 18 7 g Nitzschia dissipate 9 21 2 22 6 e Etrachia filiformas 16 17 E Nitzschaa pales 16 14 7 46 9 7 Pfeoicosphenia curvata 5 4 2 23 ~ C steletonema potamos 12 44 36 7,066 1,324 199 714 72 Surirella ovata 9 14 2 4 7 23 > tw synedra planktonica 5 7 4 7 6 9 17 2 "E syrrara ovella 50 5 2 UM ]g small centries 111 204 155 444 149 662 2,318 1,514 861 1,326 576 265 -o $ *= CRTFTOFETD E Cryptonones crosa 5 21 12 32 5 17 155 9 s1 25 30 32 UU Rhodomonas minuta 83 34 346 14 74 235 39 18 4k >M t- > MICJto?m*TES 111 252 1,348 876 1,311 2,815 2,539 2,743 1,059 1,479 2,089 1,923 _g b h tal Phytoplankton 6,502 1,570 1,999 2,121

• 804 4,536 15,464 8,138 4,592 5,9 56 3,293 2,760

@wd Total of Most Abundant Tata 5.540 1,355 1,967 2,005 1,767 4.496 15.038 8,e41 4,474 5,694 3,229 2,732 Percent Composition of Pbst Abundant Phytoplankton 85 86 99 95 98 99 97 99 97 96 98 99 l

DUQUESNE LIGl!T COMPANY 1969 ANNUAL DIVIROt4HENTAL REPORT ferences in the phytoplankton community batween 1989 and the previous years were due to natural fluctuations and were not a result of BVPS operations. Shannon-Weiner, evenness, and richness diversity values wore unusually low in January when the phytoplankton counts were dominated by two col-onies of blue-green algae. Yearly mean Shannon-Weiner diversity indices from 1973 through 1989 were similar (except during 1973 when the value was much reduced) ranging from a low 2.67 in 1989 to a maximum of 4.36 in 1975 (Table V-C-4). Yearly mean evenness values were also similar, except during 1973 when the value was low. From 1974 through 1989, evenness ranged from 0.29 to 0.90. The maximum evenness diversity value is 1.0 and occurs when each species is represented by the same number of indi-viduals. The mean number of taxa cach year ranged f rom 19 in 1973 to 49 in 1986 (31 in 1989). The highest number of taxa (60) in phytoplankton samples occurred during November, 1986. Summary and conclusions The phytoplankton community of the Ohio River near BVPS exhibited a sea-sonal pattern similar to that observed in previous years. This pattern is cot non to temperate, lotic environments. Total cell dennities were within the range observed during previous years. Diversity indices of phytoplankton were within the range of those previonly observed near BVPS. Diatoms, green algae, and microflagellates wete the most abundant phytoplankton groups in 1989. D. ZOOPLANKTON Objectives Plankton sampling was conducted to determine the condition of the zoo-plankton community of the Ohio River in the vicinity of the BVPS. 44 J

i 7ABl.E T-C'6 PETTor1ANCON DIVERSITT INDICES (MEAN OF ALL SAMrLES 1973 TO 1989) NEW Cure!ERIAND POOL OF TEE CHIO 8tIVER EvFS Jon Feb Mar Apr May Jun Jt{ Aug Sep Oct Mw

Dee, 1973 Mamber et Species 7

2 (d) 13 24 27 28 30 24 17 16 19 .Shannon Index *8 1.55 0.54 0.63 1.64 2.28 3.55 3.72 3.37 3.25 3.27 2.35 I Evenness 0.33 0.15 0.11 0.15 0.35 0.55 0.52 0.50 0.54 0.53 0.38 fuchness 1.24 0.29 1.50 2.63 3.17 3.61 3.46 3.24 2.89 2.80 2.48 l 1974 Wumber of Species 12 8 17 22 44 46 47 60 34 47 34 Shannon Inden 2.96 2.23 3.18 3.50 4.89 4.40 4.03 4.25 3.85 5.02 3.83 Evenness 0.55 0.46 0.57 0.58 0.62 0.62 0.56 0.55 0.54 0.58 0.56 Richness 2.55 1.82 3.05 3.74 5.56 5.45 5.46 6.49 4.77 5.44 4.43 g to 'O 1975 N2mber Cf Spaeles 52 34 43 32 40 40 g$ ty S snnon Index 4.53 4.22 4.37 4.22 4.48 4.36 g Evenness .80 0.83 0.81 0.87 0.35 0.83 CC Richness M 3.96 4.98 3.92 6.19 4.91 [Q j 1976 E" Number of Species 31 35 31 38 47 49 46 43 38 33 35 38 39 < t* Shannon Indem 3.98 4.36 3.90 4.25 4.14 4.27 4.28 4.30 3.93 4.16 4.24 4.45 4.19 Evenness 0.P9 c. 0.78 0.81 0.75 0.76 0.78 0.83 0.75 0.83 0.83 0.85 0.80 Richness 5.15 5.8, 4.92 4.70 4.68 4.79 4.72 4.34 3.85 4.17 d.95 5.79 4.83 5gO 5 b 1977 $h Neseer of Species 20 28 31 24 36 30 44 39 37 32 33 27 32 Shannon Index 1.96 3.31 3.00 2.78 4.16 3.52 4.36 4.26 4.29 3.92 4.12 4.00 3.64 Evenness 0.44 0.70 0.61 0.60 0.80 0.72 0.80 0.81 0.82 0.78 0.82 0.83 0.73 k* Richness 3.14 4.57 4.44 2.95 3.53 2.77 4.63 4.25 3.87 3.98 4.18 3.72 3.84 g

D

• 3 1978 Number of Species 3

29 32 42 29 42 36 37 35 37 34 32 35 i Shannon Inden 4.08 3.68 3.77 4.67 3.30 4.16 3.95 4.17 3.81 3.99 3.80 4.44 3.99 Evenness 0.78 0.76 0.76 0.87 0.69 0.78 0.77 0.80 0.76 0.77 0.76 T.90 0.78 Richnessib) 4 1979 Number of Species 18 16 19 36 34 27 34 24 29 23 28 38 27 Shannon Index 3.49 3.36 3.79 3.22 3.78 3.84 4.10 3.88 4.12 4.07 3.68 4.32 3.80 Evenness 0.84 0.82 0.88 0.62 0.74 0.81 0.80 0.84 0.84 0.88 0.77 0.83 0.81 Richness 2.97 2.64 3.36 4.69 4.08 2.98 3.46 2.72 3.26 3.52 3.57 5.19 3.54 i 1980 Ec) Nunoer of Species 28 18 24 25 21 18 30 16 32 24 33 37 24 Shannon Index 3.88 2.64 3.78 3.82 3.28 3.26 3.61 3.45 4.10 3.54 3.73 4.56 3.57-Evenness 0.81 0.64 0.83 0.82 0.75 0.78 0.74 0.86 0.82 0.77 0.74 0.87 0.78 Richness 4.07 2.55 3.49 4.02 2.50 2.38 2.90 1.94 3.33 2.59 4.01 5.40 3.15 l

TAaLE V-C-4 (Continued) Jan__ Feb mar Apr May. Jan Jul Aug Sep Oct Wow Dee I 1 1981 3 Number of Species 22 35 37 39 34 33 33 51 35 27 40 32 35 t Shannon Indem 3.92 4.39 4.39 2.29 3.66 4.56 4.13 4.59 4.07 3.90 4.00 4.32 3.95 i Evenness S.88 0.85 ~0.84 0.43 0.72 0.90 0.82 0.81 0.79 0.82 0.75 0.86 0.79 Richness 3.91 5.84 6.10 4.58 3.69 4.61 3.73 5.75 3.85 3.56 5.00 4.55 4.60 i 1902 i Number of Species 51 41 46 22 55 44 66 54 53 35 50 49 47 i Shannon Inden 4.58 4.80 4.96 L88 4.79 4.33 4.72 4.54 4.22 3.97 4.09 4.66 4.30 Evenness 0.82 0.90 0.90 0.42 0.83 0.79 0.78 0.79 0.74 0.77 0.72 0.83 0.77 y 4 Richness 7.17 6.43 6.88 2.36 6.15 4.96 6.65 .5.33 5.23 3.61 5.36 6.23 5.53 eee 1983 i Number of Species 36 42 51 52 25 42 37 40 37 45 37 52 41 l Shannon Index 4.27 4.01 4.60 4.74 3.57 4.41 4.16 4.28 3.56 3.51 4.17 4.72 4.18 ZO Evenness 0.82 0.74 0.81 C.83 0.79 0.82 0.80 0.80 0.68 0.64 0.80 0.83 0.78 5 Richness 5.17 6.45 7.35 6.64 2.98 4.18 3.63 4.17 3.83 4.46 4.38 6.48 4.98 g G tJ 1984 gp 1 m Numoer of Sp Mies 31 60 36 46 41 51 57 54 51 93 54 44 48 Shannon Indem 4.02 4.89 4.30 3.06 4.37 4.48 4.34 4.03 4.38 4.00 4.59 4.10 4.11 8$ Evenness 0.80 0.83 0.82 0.55 0.81

0. F9 0.74 0.70 C.77 0.70 0.80 0.75 0.76 2 *3 hn Richness 5.05 8.95 6.54 6.99 5.55 6.

7.29 5.97 5.43 5.70 7.10 6.71 6.47 1985 Number of Species 41 38 53 39 46 52 53 58 50 El 50 39 48 PE l Shannon Inder 3.80 3.31 4.44 3.88 4.24 2.95 4.16 4.28 3.59 2.57 3.15 3.25 3.56 gE Evenness 0.71 0.63 0.78 0.56 0.77 C.52 0.72 0.73 0.63 n 43 0.55 0.61 0.64 Richness 6.42 5.75 8.48 5.25 4.71 5.12 6.83 6.14 5.40 6.09 6.70 5.88 6.05 0

o

+3 i 1986 Number of Species 31 39 42 34 45 60 56 48 60 54 '8 48 49 i Shannon Index 3.79 4.48 3.73 1.50 4.04 3.78 4.04 3.94 4.2A 4.01 4.44 4.40 3.86 Evenness 0.77 0.85 0.69 0.29 0.74 0.64 0.69 0.70 0.71 0.70 0.73 0.79 0.69 Richness 4.54 6.40 6.32 3.72 4.54 7.37 6.20 4.75 5.16 6.34 9.58 7.99 6.14 I i 1987 7 Number of Species 42 44 29 33 33 36 50 39 33 36 35 31 37 Shannon Index 2.99 2.28 2.51 1.89 3.38 3.56 3.76 3.44 2.12 2.52 2.54 2.41 2.78 Evenness 0.55 0.41 C.52 0.37 0.67 0.69 0.67 0.65 0.42 0.48 0.50 0.88 0.53 Richness 5.24-5.58 3.J4 3.71 L.36 3.67 4.80 3.77 3.11 3.93 3.80 3.79 4.00 l

TAB 1.E V-C-4 (ContinM) w Jan Feb_ Mar Apr May Jan Aug Sep_ Oct For, _ D*e X e 1988 Nwaber of Species 31 34 27 40 45 26 42 42 37 37 36 27 35 $b Shannon Index 3.27 1.90 1.72 2.68 2.53 2.81 3.76 3.13 3.76 2.30 2.61 2.65 2.73 Evenness 0.64 0.37 0.36 0.50 0.51 0.61 0.70 0.58 0.72 0.44 0.50 0.56 0.54 g$ t7 Richness 3.43 4.21 3.28 4.65 4.75 2.66 4.20 4.12 3.72 3.25 3.83 3.00 3.76 C.M 1989 Q g. Number of Species 27 46 25 45 26 25 37 29 24 30 34 29 31 Shannon Index 1.36 4.32 2.00 3.26 1.81 2.11 2.80 3.01 3.70 3.53 2.16 1.25 2.67 bO Evenness 0.29 0.78 0.43 0.60 0.38 0.45 0.54 0.62 0.81 0.72 0.42 0.40 0.54

• i Richness 2.96 6.12 3.16 5.74 3.33 2.85 3.73 3.11 2.85 3.34 4.07 3.53 3.73 0

5> tB I"3 Shannon-48einer Index (b)1eo data gE ICI Data for period April 1980-December 1989 represents single entrair:sent samples collected monthly. m (d) Blanks represent periods when no collections were smoe. O +9 i I

DU9UESHE LIGHT COMPANY 1989 ANNUAL ENVIRCWMENTAL REPORT Methods The zooplankton analyses were performed on one liter aliquots taken from the preserved one-gallon samples obtained from the intake uay. (see Phytoplankton methods, in Part C). One lit.er from each sample was fil-tered through a 35 micrometer (.035 mm) mesh screen. The portion retained was washed into a graduated cylinder and allowed to settle for a minimum of 24 hours. The concentrate was adjusted to a known volume by removing the supernatant. One m). of this thoroughly mixed concentrate was placed in an inverted microscope cell and examined at 100X magnifica-tion. All zooplankters within the cell were identified to the lowest practicable - taxon and counted. Total density (individuals / liter), Shannon-Weiner (log base 2) and evenness diversity indices (Plelou 1969), and richness index (Dahlberg and Odum 1970) were calculated based upon one sample, which was collected below the skimmer wall from one operating intake bay. seasonal Distribution The zooplankton community of a river system is typically composed of protozoans and rotifers (Hynes 1970, Winner 1975). The zooplankton com-munity of the Ohio River near DVPS during preoperational and operational sonitoring years was composed primarily of protozoans and rotifers. t Total organism density and species composition of zooplankton from the Ohio River and entrainment samples were similar during 1976, 1977, 1978, and 1979 (DI4 1980). Samples collected from intake bays were usually representative of the zooplankton populations of the Ohio River, near BVPS. During 1989, protozoans and rotifers accounted for 73% or mare of all zooplankton on all sample dates (Table V-D-1). Total organism densities during the winter and early spring (January through May) were less than 900/ liter (Figure V-D-1, Table V-D-1). Total organism densities did not peak until September (5,420/ liter); thereafter densities decreased grad-ually until December. The maximum zooplankton density in the Ohio 48

TABLE V-D-1 MONTHLT ZOOPLANKM GROUP DENSITIES (Number / liter) AND PERCENT COMPOSITION FROM ENTRAINMENT SAMPLES, 1989 B7PS Jan Feb Mar Apr May June Group f/L 1 f/L 1 f/L 1 f/L f/L 4 f/L 1 Protozoa 680 96 795 93 780 95 780 88 705 96 2200 94 Rotifera 30 4 60 7 45 5 90 10 30 4 140 6 E EU Crus tacea 0 0 0 0 0 0 15 2 0 0 0 0 gh Total 710 100 855 100 825 100 885 100 735 100 2340 103 E E" SC Jul Auc Sep Oct Nov Dec b5 Group t/L f/L t/L 1 t/L 1 f/L 4 f/L 1 5" E2 O Protozoa 2910 87 400 22 3000 55 1575 79 90C 94 430 91 g Rotifera 420 13 920 51 2360 44 390 20 60 6 40 9 N* o Crustacea 0 0 480 27 60 1 30 2 0 0 0 0 N Total 3330 100 1800 130 5420 100 1995 101 960 100 470 100 i } E

iiilil! l o8EE" C@" n955 Gg >EE" ES85e4E wEo4 II' C YN u E N D 9 -N 8 NN V 9 1 N4 O N C RE T V AW-I C R O 8 D 8 ON 9 IA q P 1 H W r O) S E 6 5R j S 7 E7A N 9 H9E / 1 G T1Y E w U A G N4) .WN A I7998 R 1 S19 l L E E( 1 UH A D i f / JT A IL6 V N V AA7S SN9P / N O E NO1V I A UM R EI( B 6 U DT J G AL / I NRA Y 8 F OEN I PO 4~ [ A R C OI i / I M Y NET / ARA / ~- L LPR R A P E = P N OGP A O ONO ZI I y T R R A YU /' ^ A R LD m e / M E HT P N R O O E w B E M T 7 E R I F P L 7r Q / N 0 S A L J LEC 0 0 0 0 0 0 0 0 0 1 0 1 1 ,o

DUQUESHE LIGiff COMPA11Y 1989 ANNUAL INVIR0tiMENTAL REPORT River near DVPS frequently occurs in the spring, although it is sometimes delayed until summer or early f all (Table V-D-2, Figure V-D-1). Optimum conditions of low precipitation and warm w.ather did not occur until late summer when the peak occurred in 1989. The effect of a dry year and low river discharges was noted by Hynes (1970) to favor plankton populations. The ceasonal pattern of zooplankton densities observed in the Ohio River near DVPS is typical of those in temperate climates (llutchinson 1967). Zonplankton denalties in winter are low due primarily to low water tem-peratures and limited food availability (Winner 1975). In the spring, food availabililty and water temperatures increase, which stimulates growth and reproduction. zooplankton populations decrease during the f all and winter f rom the summer maximum because optimum conditions for growth and reproduction decrease during this period. Demitler of protozoans during Jcnuary through May of 1989 were between L ud 795/11ter (Table V-D-1). Protozoans increased in June and July; decreased in August and developed the hig'

• L densities of 1989 in September.

Protozoans gradually decreased in e f all to densities of 430/ liter in December. Vgrticella sp., Tintinnidium fluvitale, Strombidium sp. and Dif flugla sp. were the common protozoans throughout the year. Vortice11a sp. or Difflugia sp. dominated the protozoan assemblage during nine months (Table V-D-3). The most abundant proto-zoans in the other months were Tintinnidium, Tintinnopsis and Strombidium. These taxa have been a main part of the protozoan assem-blage of the Ohio River near BVPS since environmental studies were initi-ated in 1972 by DLC. The rotift,r assemblage in 1989 (Figure V-D-2) displayed a typical pattern of rotifer populations in temperate inland waters (liutchinson 1967). Rotifer densities increased from a minimum of 30/11ter in January to a maximum of 2,360/11ter in September a secondary peak did not occur in 19S9 (Table V-D-2).- Rotifer populations decreased after September to densities of 40/11ter in December. Rotif ers were the second most abun-dant group during 1989. Keratella cochlearls and Polyarthra dolichoptera were the most abundant rotifers during most of the year (Table V-D-3). 51

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l I NUMBER / LITER 3500 l n 3000= 2500 g 3 2000 j / E8 / EE pg l 1500- = l <r @E l = 1000 g \\-\\ Sk = :s [ $n 500 D r> ^ ^ m a i i i i i i i i i p t_ O, JAN FEB .A A R APR MAY JUN JUL AUG SEP OCT NOV DEC MONTH CRUSTACEA 0 ROTIFERA C PROTOZOA FIGURE V-D-2 ZOOPLAN1GON GROUP DENSITIES EVR ENTRAINMENT SAMPLES, 1989 BVPS s..

t

[!ifhtFf' f L ffFf tL\\Lfi!iIi jl FI!!!$I (i-ltI' ,L f[- [i [* f{ 3i((jt j; ' ,ht I QoCE7" U 3 $25 r $ g ;+ 1 F s po$ &ECh $<"h0Kh::yE ,6Wd t t ,~ t 0 00 0 . 00 00000 0 0e0 0 0 0 t 1 21 2 13 21253 1 1l1 7 7 0 I 2 4 4 1 h 0 0 e0 0 0o00 000 0a 0 0 0 4 6 44 6 2t44 402 4 *. 6 6 0 1 11 1 9 9 1 1 0 5' 0 050550 05 00 0 5 5 0 E 2 1 2 869373 94 36 3 9 9 0 S 1 1131 3 3 9 9 1 1 1 t 00 0 0 0000 000 000 00 0 0 6 s 32 9 3 4 4s2 24 0 342 24 2 0 9 I 1 3 01 371 63 4 2 11 5 5 ~ 1= 2 4 4 - 00o0 0 000 00 00 0 0 7 0 0 0 33t2 2 024 42 68 0 4 9 ^_ 1 12 4 3 5 7 1 1 A W RA' T t l. 0 00 0. 000000 00 00o 0 0 6 i 3 63 4 136969 99 95t 3 1 9 s t f 33 2 33 9 1 3, 2 n 3 3 iu J 1F S 0 .A 0 1 9 0000 00 0 000. 000 0 0 0 0 0 0 i l 2 aF9 L* 2244 44 2 64 0 24 2 6 2 2 4 0 9 t p9 3 4 1' 162 1 3 3 T a1-a fs 6 S 2 2 a rD m yr n E aeS U 3 A DB I 5 5 00 5 050 05 5 5 5 5 0 2 ns p re2C = 1 7 37 1 649 91 1 1 3 3 0 AE 3 2 7 7 1 g v 4 RSs e2rT w r s nBgs g e r r e a Oet o a hs n v tee t 5 50 5 0 050 055 05 5 5 0 0 ) 1 r FT t 1 16 5 3 312 354 34 1 9 7 9 e r A_ 1 1 2 9 8 te tr leNI t i lA e / D r r s eG A b( J 2'_ 0 55 S 5050 055 5 5 5 5 6 m 3 14 I 1911 354 1 1 2 9 9 a t 2 2 8 7 I( SE IT I d 0 0 55 50 55 0 5 5 5 5 0 S 3 9 74 13 64 3 1 1 5 5 0 t5 I 3 1 2 8 1 D t 0 ' 0O000 00 00 0 0 0 0 7 E 1 1S211 23 53 1 1 1 9 9 2 I 3 7 6 s t ,e e m t a n z o a a m a f t e c f r a ' T ok e r n l i t d u t na a r .d s a s s t d e i i i p e y n ol a .e n i n i n r r a o i h e i p a .l t s a v a f r a a t h f c d t o r pl a e r . e l . i . o e e a c i a m i o r a t r p n st e su .i l y p .l y .t pe l d d l n b 0 a o p t p l p t s pf c = n st h t a o ppe n c e a o .e 7 A pt s e m e ...ict m m m n ssd a e o o u d pd n K an r u s pp T .s e t sop s u u m u a i l c c q si a I t ua B l h a so i i u i e a a m l s e n c A s Cd @i o n a ea c i a s i a d d i d p h 1 i e e a a e r a u s I e eu r l i r n i a i i i d a o c 1 d l l 1 1 h t o F p a p el r e g l n n r l l i n n a e e o i l 1 1 t e r A n 1 O 9b n l o t e t d o y p a t i b n n r c t A l a e e e r a e E a 1 O f aA o l r an o o l t y ;w e o b m a i a i a t t t a h f C h 1 2 e t D ~ a a a a y c i A p p nt n e t ao A h f e l l o o o t t t t i h n t T a u L L ea c n 1d c p r i c l e r r r n n o r l F p r r r l m o S i a A A co e e e o y i D r e 1 o y y 1 p uo o t t t a a ir e a I e ACCCCCDEEmMS6sTTt v C T C c R s s P S s t DM T T rfl m O a O O e I C T T P I ic*

1 DUeUESNE LIGHT COMPANY l 1989 ANNUAL DNMONMENTAL REPORT Crustacean densities were low (0 to 480/ liter) throughout 1989 (Table V-D-1). Most c'estaceans were collected during surnmer especially August when densities were 480/ liter (Figure V-D-2). Except during August, crustacean densities never exceeded protozoan cr rotifer densities and constituted from 0 to 2% of the total zoopl ankton density each month (Table V-D-1). Copepod narlii were the most aumerous crustaceans col-lented during 1989. Crustact populations did not dmlop high dens ~ ities due to unf avorable flow end turbidity conditions in the river dur-ing most of 1989. Crustaceans are rarely numerous in the open waters of rivers and many are eliminated by silt and turbulent water (Hynes 1970). The highest Shannon-Weiner d!versity vilue of 3.49 occurred in November while the maximum number of. species (22) occurred in September (Table V-D-4). Evetiaess ranged from 0.62 in January to 0.92 in November. Richness varied from a low of 1.48 in February to a high of 2.67 in August. The number of species ranged from 11 in February to 22 in September. Diver-sity indices were relatively high during all months of 1989. 4 Compa: icon of Control and Non-Control Transects Zooplankton sampion were not collected from stations on the Ohio River after April 1, 1980; therefore, cesparison of Control and Non-Cont rol Transects wu .ot possible. Comparison of Preoperational and Operaticnal Data Population dynamics of the zooplankton community during the seasons of preoperutional and operational years are displayed in Figuic V-D-1. Total zooplankton densities were lowest in winter, usually greatest in summer, and transitional in spring and autumn. This pattern in the Ohio River sometimes varies from year to year whicn is normal for zooplankton populations in other river habitats. Hynes (1970) concluded th a t the zooplankton cormnunity of rivers is inherently unstable and subject to constant char 4e due to variations of temperature, flow, current, tur-bliity, and food source. Total densities of zooplankton during 1989 were within the range established during the preoperational years (1973 through 1975) and operationel years (1976 through 1988) (Figure V-D-1). l 56

TABLE V-D-4 ZOOPLANKTON DIVERSITY INDICES BY MONTH FOR ENTRAINMENT SAMPLES,1989 BVPS Jan Feb Mar Apr May Jun No. of Species 14 11 15 15 12 18 w" Shannon-Weiner Index 2.37 2.68 3.02 3.22 2.91 3.21 3 2: O Evenness 0.62 0.77 0.77 0.82 0.81 0.77 ES CC5" Richness 1.98 1.40 2.08 1.92 1.67 2.19 E tg to Jul Aug Sep Oct Nov Dec X C b"O No. of Species 18 21 22 14 14 15 16 !!i!DG ~9 Shannon-Weiner Index 3.43 3.46 3.35 3.20 3.49 2.82 3.10 $E t' 2: Evenness 0.82 0.79 0.75 0.84 0.92 0.72 0.76 5 8 Richness 2.10 2.67 2.33 1.71 1.89 2.28 2.03 E i g

DUQUESNE LIGiff COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT The data for 1989 indicate that the peak zooplankton densities were delayed until September due to unfavorable flow and turbidity conditions in the river. The species composition of zooplankton in the Ohio River near BVPS has remained stable during preoperational and operational ;aars. The common or abundant protozoans since 1972 have been Vortice11a, Codonella, Difflugia, Strobilidium, Cyclotrichlum, Arcella and Strombidium. The most numerous and frequently occurring rotifers have been Ker a t e ll a,, Polyarthra, Synchaeta, Branchionus and Trichocerca. Copepod nauplit have been the only crustacean taxon found corsistently. Community structure, as compared by diversity indices, has been similar since 1972 (Table V-D-5). In previous years, low diversity indices ard number of species occurred ir. winter s high diversities and number of species usually occurred in late spring and summer.

• ~

In 1999, the divercity indices and species numbers were relatively low in January and February which was typical for months of winter and early spring. Shannon-Wiener diversity indices in 1989 ranged from 2.37 to 3.49 and were _ slightly higher than the range of 1.80 to 3.28 that occurred during preoperational years from 1973 to 1975. The variation in evenness during 1989 (0.62 to 0.92) was at the upper portion of the range reported from 1973 to 1988 (0.21 to 0.93). Summary and Conclusions Zooplankton densities throughout -1989 were typical of the temperate zoo-plankton community found in'large river habitats. Total densities were within the range of those reported in preoperational and several opera-tional years. Populations developed highest densities in September. Except during August, protozoans and rotifers were always predominant. Common and abundant taxa in 1989 were similar to those reported during l. preoperational and operational years. Shannon-Weiner diversity, number of species, and evenness were within - the ranges of preceding years. Based on the data collected during the 14 operating years (1976 through 1989) and the three preoperational years (1973 through 1975), it is 58 l

i. TnsLE V-D-5 sEAN 3CJFIANE708 DIVERSITY INDICES ST MuttTE FROM 1973 TEROC211999 IN TEE 0520 R.IVER NEAR BVPS ~ Jan Feb . ster Apr May Jan Jul Aaq sep Oct New Dee -1973 gay Number of 8 e1 8.44 15.29 21.28 25.07 : 21.96 '22.06 16.33 14.40-14.30 'Shannon Inden 1.00 '3.06 3.08 7.79 2.25 2.20 2.21 '2.31-3.10 Evenness' O.37 0.63 0.53 0.46 0.39 0.36 0.37 0.44 0.61 5 1974 Number of Specits. 14.64 9.18 14.92 17.75 23.25 15.56 21.14 18.09 9.56 14.47 1 Shannon inden 3.10 2.53 -2.91 3.06 3.25 2.32 3.20 2.24 '2.15 1.84-Evenness 0.62 0.56 0.57 0.58 0.55 0.41 0.60 0.41 0.42 0.30 -- 1975 Number of Species. 24.75 18.75 14.38 17.44 15.38 Shannon Inden 3.20 1.86 2.90 2.01 3.20 Evenness-0.69 0.$4 0.77 0.49 0.82 i $8 f 1976 Number of Species 7.00-0.13 3.69 17.56 19.19 23.56 23.06 23.50 23.56 11.19 8.75 11.75 2O i Shannon Inden 1.67 2.64 2.24 0.89 3.06 2.33 3.36 3.63 2.76 2.73 1.60 2.64 CC' I Feenness 0.60 0.84 0.73 0.21 0.72 0.51 0.70 0.80 0.61 0.79 0.51 0-75 b 1977 Number of species 4.00 10.00 12.00 13.31 21.00 25.62 22.88 25.50 36.75 16.se 20.31 15.31 ',[ ~ in Shannon Inden ,1.53 2.59 3.01 2.99 3.15 3.45 3.32 3.60 3.71 3.35 3.42 3.42 f g C3 Evenness 0.78 0.79 0.87 0.81 0.72 0.74 0.73 0.77 0.71 0.82 0.79 0.s6 2g 1978 M3 - [ Number of Species 0.12 7.12 4.31 5.12 7.62 6.25 10.25 11.25 12.50 0.25 10.88 10.3s y ( Shannon Inden 2.48 2.41 1.53 1.70 1.53 1.33 2.50 2.44 2.53 2.28 2.15 2.00 i Evenness 0.83 0.85 0.14 0.71 0.52 0.50 '.76 0.70 0.70 0.73 0.62 0.e3 i @M 1979 [ 9' Number of Speeles 10.62 6.00 10.25 15.88 17.25 14.25 16.99 21.50 18.12 12.00 14.62 14.00 o Shannon Inden 2.51 2.52 3A5 3.42-2.36 3.02 2.42 3.30 3.?6 2.99 2.84 3.10 I Evenness 0.74 0.93 0.90 0.56 0.58 0.80 0.60 0.74 0.80 0.s4 0.74 0.33 [ l ICI 1980 number of rnecies 11.52 11.00 12.50 10.00 s.00 15.00 21.00 15.00 18.00 22.00 18.00 18.00 Shannon Inden 2.51 2.70 3.01 2.41 2.00 2.!'1 3.63 2.79 3.23 2.88 3.26 3.36 [ Evenness 0.70 - 0.78 0.04 C.72' O.66 0.74 0.82 0.71 0.77 (;.6 4 0.78 0.90 I 1931 mumber of Species 8.00 12.00 7.00 11.00 19.00 12.00 13.00 24.00 20.00 21.00 17.00 10.00 I Shannon Inden 2.14 3.02 2.20 2.32 3.44 2.73 2,96 3.55 2.62 3.05 2.66 2.47 Evenness 0.71 0.04 0.91 0.47 0.81 0.76 0.65 0.77 0.60 0.69 0.65 0.74 t .P g

TABLE V-D-5 (Continued) Jan Feb Mar Apr May Jun Jul_ Atr; Sep Oct. _ We' Dec 1982 Nunner of Species 10.00 9.00 11.00 22.00 27.00 20.00 37.0C 36.00 40.00 34.00 19.00 17.00 Shannon Index 2.99 2.22 2.89 3.59 2.46 3.20 3.82 4.28 3.86 3.09 3.54 3.14 Evenness 0.90 3.70 0.83 0.80 0.52 0.74 0.73 0.03 0.72 0.61 0.83

0. 77 1983 Nan: Der of $pecies 18.00 10.00 23.00 14.00 17.00 24.00 34.00 30.00 37.00 33.00 17.00 18.00 Shannon Index 3.20 2.39 2.41 3.09 3.54 2.36 3.56 2.65 3.92

• 43 3.28 3.54 Evenness 0.76 0.71 0.53 0.81 0.06 0.51 0.70 0.54 c.73 0.68 0.80 0.85 1984 N.uber of Species 17.00 10.00 7.00 12.00 13.00 18.00 12.00

18. 0')

23.00 19.00 14.00 11.00 L.annen Indes 3.29 2.64 0.82 2.10 2.26 2.63 2.40 2.28 3.62 2.84 2.89 2.52 Evenness 0.80 0.79 0.28 0.63 0.61 0.63 0.67 0.54 0.80 0.67 0.74 0.72 He cm 1985 e Number of Species 13.00 12.00 9.00 10.00 16.00 19.00 18.00 32.00 27.00 20.00 19.00 13.00 g Shannon Index 2.32 1.98

1. 72 1.64 2.90 2.91 3.35 3.60 3.72 3.27 3.25 1.97

. c Evenness 0.62 0.55 0.53 0.49 0.72 0.68 0.80 0.72 0.78 0.76 0.76 0.53 $O g > t1 1986 E' $ - Number of Species 12.00 13.00 15.00 19.00 21.00 22.00 23.00 26.00 32.00 17.00 15.00 21.00 g$ Sharson Indez 2.97 2.84 3.13 3.15 2.26 3.74 2.94 3.69 4.19 2.90 2.83 3.10 ~ Evenness 0.83 0.76 0.80 0.14 0.74 0.84 0.65 0.78 0.84 0.71 0.72 0.70 $C m O hy 1987 NumDer of Species 13.00 14.00 16.00 14.00 9.00 20.00 28.00 25.00 20.00 20.00 16.00 16.00 N Shannon Inden 2.64 1.76 3.40 3.54 0.89 3.15 3.53 3.50 3.29 3.37 2.32 3.48 GO $h Evenness 0.71 0.46 0.85 0.93 0.28 0.73 0.73 0.75 0.76 0.78 0.58 0.87 >9 1988 NumDer of Species 8.00 17.00 17.00 13.00 13.00 24.00 14.00 24.00 26.00 22.00 16.00 21.00 g '< Shannon Inden 2.45 2.57 2.70 2.30 2.60 3.30 2.J9 3.20 3.48 2.35 2.97 2.68 g Evenness 0.82 0.62 0.65 0.62 0.70 0.72 0.60 0.70 0.14 0.53 0.74 0.61 4 1989 Number of Species 14.00 11.00 15.00 15.0S 12.00 18.00 18.00 21.00 22.00 14.00 14.00 15.00 Shannon Indez 2.37 2.68 3.02 3.22 2.91 3.21 3.43 3.46 3.35 3.20 3.49 2.82 Evenness 0.62 0.77 0.77 0.82 0.81 0.77 0.82 0.79 0.75 0.84 0.92 0.72 I*I slanks represent periods when no collections were mass. IDI Shannon-weiner Inder I*I Data for period April 1980-December 1989 represents single entrairament samples ecliected monthly.

se DUQUESNE LIGl!T COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT concluded that the Overall abundance and species composition of the zoo-plankton in the Ohio River near BVPS has remained stable and possibly improved slightly over the seventeen year period from 1973 through 1989. The data indicate that increased turbidity and current from high water conditions have the strongest effects of delaying the populations' peaks and temporarily decreasing total zooplankton densities in the Ohio River near DVPS. E. FISil Objective Fish sampling was conducted in order to detect any changes which might occur in fish populations in the Ohio River near DVPS. Methods Adult fish surveys were performed in May, July, September, and November 1989. During each survey, fish were collected at the three study trans-ects (Figure V-E-1) using gill nets, electrofishing and minnow traps. The gill nets consisted of five 25-f t. panels of 1.0, 2.0, 2.5, 3.1 and 3.5 inch square mesh. Two nets were positioned close to shore .c each transect, with the small mesh irshore. As Transect 2 is divided by Phillis Island into two separate water bodies consisting of the main river channel (2A) and the back channel (2B), south of the island, a total of eight gill nets were set per sampling month. Nets were set for approximately 24 hours. All captured fish were identified, counted, measured for total length (mm), and weighed (g). Electrofishing was conducted with a boat-mounted boom electroshocker. Direct current of 220 volts and (ne to two amps was generally used. Shocking time was maintained at 10 minutes per transect for each survey. l The shoreline areas of each transect were shocked and large - fish pro-ceased as described above for the gill net collections. Small fish were l immediately preserved with 10% formalin and returned to the laboratory 61 l l

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= DUQUESNE LIGi!T COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT for analysis. Non-game fish were counted and a batch weight obtained for the entire sample. The length range was determined by visual inspection and measurement of the largest and smallest fish. N Minnow traps were baited with bread, cheese, and sucrose and placed next to the inshore side of each gill net on each sampling date. These traps were painted black and brown with a camouflage design and were set for 24 hours. All captured fish were preserved and processed in the laboratory in the manner described for electrofishing. Results Fish population studies have been conducted in the Ohio River near BVPS from 1970 through 1989. These surveys have collected 64 fish species and two hybrida (Table V-E-1). In 1989, 31 fish species were collected. Grass carp, which had not been collected in previous years, was collected in 1989. A combined total of 865 individuals were collected in 1989 by gill netting, electrofishing and minnow traps (Table V-E-2). A total of 706 fishes, representing 19 species were collected by electro-fishing (Table V-E-3). Collectively, shiners accounted for 54.7% of the total electrofishing catch in 1989. Gizzard shad, also a forage species, represented 31.4% of the catch. Carp and white bass accounted for 2.34 and 3.3% of the catch. Smallmouth bass and golden redhorse accounted for 1.7% and 1.3% (combined bass spp. accounted for 7.2% of total catch). Each of the other taxa accounted for less than 1% of the total. Most of the fish sampled. by electrofishing were collected in September (4 4. 5%). The fewest fish were collected in Tuly (15.3%). It should be noted that " observed' fishes were included in the catch per unit effort. This was sometimes necessary because of the turbidity and swiftness of the high water. Since the netters could not physically collect these stunned fishes, they were identified to the genus level and recorded ar " observed". 63

DUQUESNE LIGi!T COMPANY 1909 ANNUAL 1:NVIRONMENTAL REPORT TABLE V-E-1 (SCIENTIFIC AND COMMON NAME)1 FAMILIES AND SPECIES OF FISH COLLECTED IN THE NEW CUMBERLAND POOL OF THE OHIO RIVER, 1970-1989 BVPS Family and Scientific Name Common Name Lepisosteidae (gars) Lepisosteus osseus Longnose gar Clupeidae (herrings) Alosa chrysochloris Skipjack herring Dorosoma cepeolanum Gizzard shad Hiodontidae (mooneyes) Hiodon tergisus Mooneye Salmonidae (salmon and trouts) Salmo gairdneri Rainbow trout i Esocidae (pikes) Esox lucius Northern pike E. masquinongy Mur.kellunge E[. lucius X E!. masquinongy Tiger muskellunge Cyprinidae (Linnows and carps) _Campostoma anomalum Central stoneroller ,Carassius auratus Goldfish Ltenopharyngodon idella Grass carp Cyprinus carpio Common carp C. carpio X C. auratus Carp-goldfish hybrid Ericymba buccata Silverjaw minnow Nocomis micropogon River chub-Notemigonus crysoleucas Golden shiner Notropis atherinoides Emerald shiner 2 N. chrysoce phalus' Striped shiner g hudsonius Spottail shiner N. rubellus Rosyface shine: ((. spilopterus Spotfin shiner }I. stramineus Sand shiner N. volucellus Mimic shiner Pimephales notatus Bluntnose minnow Rhinichthys atratulus Blacknose dace Semotilus atromaculatus Creek chub 64

am DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT TABLE V-E-1 (Continued) Family and S:lentific Name Common N.me Catostomidae (suckers) Carplodes carpio River carpsucker Carpiodes cyprinus Quillback Catostomus commersoni White sucker Hypentelium nigricans Northern hog sucker Ictiobus bubalus Smallmouth buf f alo I,. niger Black buffalo Moxostgma anisurum Silver redhorse H. car'nasum River redhorse M. duquesnei Black redhorse {l. erythrurum Golden redhorse

11. macrolepidotum Shorthead redhorse Ictaluridae (bullhead and catfishes)

Ictalurus catus White catfish I. melas Black bullhead I. r.atalls Yellow bullhead I. nebulosus Brown bullhead I punctatus Cnannel catfish Noturus flavus Stonecat Pylodictis olivaris Flathead catfish Percopsidae ( trou t-pe rches) Percopsis omiscomaycus Trout-perch Cyprinodontidae (kil11 fishes) Fundulus diaphanus Banded killifish Athe rinidae (silversides) Labidesthes sicculus Brook silverside Percichthyidae (temperate basses) Morone chrysops White bass Centrarchidae (sunfishes) Ambloplites rupestris Rock bass Lepomis cyanellus Green sunfish L. gibbosus Pumpkinseed L. macrochirus Bluegill Micropterus dolomieui Smallmouth bass !b punctulatus Spotted bass tb _salmoides Largemouth bass Pomoxis annularis White crappie P,, nigromaculatus Black crappie 65

DUQUESNC LIG6tT COMPANY 1909 ANNUAL ENVIIOllMLt1TAL REPORT TABLE V-E-1 (Continued) Family and Scientific Name Common Name Percidae (pe rche s) Etheostoma blennioides Greenside darter E. nigrum Johnny darter E. zonale Banded darter Perca flavescens Yellow perch Perclna caprodes Logperch P_. copelandi Channel darter Stizostedion canadense Sauger S. vitreum vitreum Walleye Sciaenidae (drums) Aplodinotus grunniens Freshwater drum 3 Nomenclature follows Robins, et al. (1980). 2A former subspecies of N. cornutus (Gilbert, 1964) and previously reported as common shiner. 9 0 66

TABLE V-E-2 NUMBER OF FISH COLLECTED AT VARI 0tf3 TPRISECTS BY GILL NET (G), ELECTROFIOHING (E) AND MINNOW TRAP (M) IN THE NIX CUMBEstIRED POOL OF TdE OHIO RIVER,1989 BVPS Peccent 1 2A 2? 3 _ Grand Total _ Annual Annual Taxa G E, M G, E 3 G E 3 G E M, G E M Total Total Iongnose gar 2 1 2 5 5 0.6 Girzard sbad 62 97 44 19 222 222 25.7 Mus.ellunge 1 1 2 2 0.2 Tiger :auskellunge 1 3 4 1 8 1 9 1.0 Pi..- sp. 1 1 2 2 0.2 Grass carp 1 1 1 0.1 Comron carp 8 5 15 1 2 2 14 8 39 16 55 6.4 g Golden stiner 1 1 1 0.1 <= Erectald chiner 6 10 3 3 1 20 3 23 2.7 Spottall shiner 1 2 3 3 0.3 $8 Spotfin sh. e 1 1 1 0.1 Shir:er sp. 14 64 204 80 362 362 41.8 %Q River carpsus.ker 1 1 3 1 4 5 0.6 = Quillbsck 1 1 1 1 2 0.2 E" White sucker 1 1 1 0.1 6C m" S.aa11 mouth buf falo 1 1 1 0.1 8@ Silver redhorse 1 1 1 1 2 0.2 g8 Golden redhotse 2 5 1 1 1 3 2 6 9 15 1.7 [2 O Shorthead redhorse 1 1 2 2 0.2 4 Redhorse sp. 3 3 3 0.3 $ p. Channel catfish 1 1 7 4 17 29 1 30 3.5 5 Flathead catfish 2 2 2 0.2 y White bass 1 2 2 2 1 5 18 8 23 31 3.6 O Rock bass 4 1 1 4 5 0.6 N Puntpkinseed i l 1 0.1 Bluegill 1 1 1 0.1 Sunfish sp. 1 1 1 0.1 Smallmouth bass 2 7 1 2 12 12 1.4 Spotted bass 7 5 1 10 4 8 3 1 30 8 1 39 4.5 Bass sp. 2 1 5 8 8 0.9 White creople 1 1 1 0.1 Black crappie 1 1 1 0.1 Banded darter 1 1 1 0.1 Sauger 1 1 1 4 5 2 7 0.0 Walleye 1 2 3 3 0.3 Freshwater drum 1 1 1 2 2 3 5 0.6 Total 25 108 4 38 188 18 263 4 69 147 1 150 706 9 865 g

TABLE V-E-3 NUMBER OF FISH CDLLECTED PER MONTE BY GILL NET (G), ELECTROFISHING (E), AND MINNOW TRAP (M) IN THE NIH CUMBERlAND POOL OF THE OHIO RIVER,1989 BVPS Percent May Jul Sep Nov Grand Total Annual Annual Taxa G E M G E M, G E M G E M G E M Total Total Longnose gar 2 1 2 5 5 0.6 Gizzard shad 79 79 28 36 222 122 25.7 Muskellunge 1 1 2 2 0.2 Tiger muskellunge 8 1 8 1 9 1.0 Pike sp. 1 1 2 2 0.2 Grass carp 1 1 1 0.1 Comuon carp 10 2 14 12 13 2 33 ' 16 55 6.4 w Golden shiner 1 1 1 0.1 E Emerald shiner 9 1 10 3 20 3 23 2.7 Spottall shiner 3' 3 3 0.3 Spotfin shiner 1 1 1 0.1 gQ Shiner sp. 16 2 280 64 362 352 41.8 p to River carpsucker 4 1 1 4 5 0.6 "E Quillback 1 1 1 1 2 0.2 @"3 White sucker 1 1 1 0.1 $C Smallmouth buff alo 1 1 1 0.1 ge Silver redhorse 1 1 1 1 2 0.2 ~U Golden redhorse 2 5 1 2 1 2 2 6 9 15 1.7 c Shorthead redhorse 1 1 2 2 0.2

'i 9

%f Redhor se sp. 2 1 3 3 0.3 Channel catfish 9 1 13 6 1 29 1 30 3.5 Flathead catfish 2 2 2 0.2 N White bass 4 23 3 1 8 23 31 3.6 o Rock bass 3 1 1 1 4 5 0.6 l Pumpkinseed 1 1 1 0.1 Blueg ill 1 1 1 0.1 Sunfish sp. I 1 1 0.1 Smallmouth bass 3 4 3 2 12 12 1.4 Spotted bass 19 7 1 5 1 6 30 8 1 39 4.5 Bass sp. 3 3 1-1 8 8 0.9 White crappie 1 1 1 0.1 Black crappie 1 1 1 0.1 Banded darter 1 1 1 0.1 Sauger 1 1 1 1 3 5 2 7 0.8 Walleye 1 1 1 3 3 0.3 Freshwater drum 2 2 1 2 3 5 0.6 TOTAL 58 166 4 47 108 34 314 2 11 118 3 150 706 9 865

m. DUQUESNE LIGifT COMPANY 1989 ANNUAL ENVIRONMENTAL REPOIC The gill net results varied by month with the highest catch in the month of May (50 fish). July was the next highest month with 47 fish. The November catch resulted in 11 fish. Gill not sampling typically results in catching more fish in warmer weather when fish are usually more ac tive, thus the low sample numbers encountered from November are to be expected (Table V-E-4). A total of nine fish were captured using minnow traps in 1959 (Table V-E-2). May had the highest catch with four fish. The most common species (i.e., those which contributed more than 14 to the annual total catch) collected through the use of gill nets, electro-fishing and minnow traps included the followings gizzard shad, common carp, emerald shiner. golden redhorse, channel catfish, white bass, smallmouth

bass, spotted

bass, and unidentified shiner species (observed).

The remaining species each accounted for 14 or less of the total. Comparison of Control and Non-Control Transects Comparisons of the data obtained from the Control Transect (1) with that from the Non-Control Transects indicate that the fish populations have fluctuated slightly since 1974 (Table V-E-5). However, comparisons between years include many natural variables and can be misleading. Fluctuations in catches occur with changes in the physical and chemical properties of the river's ambient water quality. Since electrofishing efficiency depends largely on the water's conductivity, any sampling conducted during extremes in tnis parameter will aff ect catch-per-unit-l effort. In addition, turbidity and.;urrent affects the collectors' abil-ity to observe the stunned fish. Direct sunlight also influences where fishes congregate, thus determining their susceptibility to being shocked. Electrofishing collects mostly small forage species (minnows and shad) a-d their highly fluctuating annual populations were reflected l in differences in catch per-unit-effort from year to year and station to station. However, gill nets catch matly game species and are more indi-cative of changes in fish abundance. When comparing gill net data (Table 69

DUQUESNE LIGil? COMPANY 1989 ANNUAL EtWIRONMENTAL REPORT TABLE V-E-4 NUMBER OF FISil COLLECTED BY GILL NET, ELECTROFIS!!ING AND MINNOW TRAP AT TRANSECTS IN Tile NEW CUMBERLAND POOL OF TIIE 01110 RIVER,1989 BVPS Transect Gill Net 1 2A 2B 3 Total Average May 10 14 8 26 58 14.5 J e.ly 9 3 6 29 47 11.8 September 5 17 3 9 34 8.5 Novembe r 1 4 1 5 11 2.0 Total 25 38 18 69 150 Average 6.3 9.5 4.5 17.3 Electrofishing May 57 48 15 46 166 41.5 July 33 38 21 16 108 27.0 September 4 12 215 83 314 78.5 November 14 90 12 2 118 29.5 Total 108 188 263 147 706 Average 27.0 47.0 65.8 36.8 Minnow Trap May 3 0 0 1 4 1.0 July 0 0 0 0 0 0 Septembe r 1 0 1 0 2 0.5 November 0 0 3 0 3 0.8 Total 4 0 4 1 9 Average .0 0 1.0 0.3 6 70

m u W TABLE V-E-5 ELECTROF1SEING CA1CS (FISE/EDUR) IRIsts d) AT TRRftSECTS IN TE Mpt CUpmmreem'ED POOL OF TEE ORIO RITER, 1974-1909 BVFO Transect 1 8 6 d S 8 d' g,;$e 1906 '1987. 39,,o'.g,,9d q] 0 d '1975b 1976 1977C '1978' - 1979C' 1980 g,gg 1992 1983 g,gg 8 Species ~ 1974

1.5 aangnose gar 3.1

1. 2 -

2.0 3.1 3.0 0.8 69.0 31.5 27.0 36.0 76.5-175.5 '33.0 Cissard shed Tiger moskellunge: -~ ~ 0.8 0.5 fauske11onge Northern pike - 1.5 1.5 '

pike op.

[ 2.3 0.8 0.7 - Goldfish 20.8 15.0 1.5 30.0 ' 54.0 13.5 9.0 15.0 18.0 ' 7. 5 Carp 5.9 - - 3.0 12.5 Rieer chub. 'g 1.5 !e 0.3 Golden shiner i Boerald shiner 42.0 '441.7 18.7 57.0. 122.s 58.4 51.5 151.5 114.8 279.0 12.0 6.0 46.5 58.5 40.5 9.0 Striped shiner 1.5 1 Spottin shiner 0.9 4.8 7.0 0.5 3.0 4.5 1.5 gg' Spotta11 shiner 1,$ 3,g g,$, go CC 't Sand shiner 57.5 129.1 52.5 95.9 S.8 93.6 32.3 .'23.2 19.5 6.0 3.0 4.5 9.0 fE Mimic shiner 3.5 7.0 - 0.5 1.6 6.2 3.0 6.0 19.5 1.5 81ontnose minnow 33.3 72.3 53.2 57.8 12.8 09.4 15.4 18.0 21.8 9.0 4.5 1.5 4.5 1.5 Creek chub 0.9 0.5 0.5 g ' :r. ^[ 19 ^ Stoneroller 2,5 , g, w Blacknose doce 4w l h.h 78.0 3.0 528.0 114.0 78.0 21.0 M Shiner op. 3: Rieer corpsucker g,$ Quillback g,$ trhite sucker 0.3 1.5 1.5 3.0 $n + o Northern hog aucker 0.7 1.0 0.3 1.5 1.5 Hg" h 'i Redhorse sp. 4.5 511eer redhorse 0.0 1.5 3.0 gM 0.8 1.0 Black redhorse Colden redhorse 1.5 1.5 1.5 4.0 1.5 - 7.5

• =2 Shorthead redborse 0.8 0.0 1.5 3.0 3.0 O

7ellow bullhead Brown bullhead l Channel catfish -~ 0.3 0.9

1. 5 1.5 -

'-L Catfish sp. Trout-pereb 1.5-0.8 1.5 Banded killifish k -L "MT-JUL h m. Ncn CMT-SEP, 507 h,.774' SEP AND Noir 'Mr. JULY, SEP AND tt0 p g

= i i TAB 1.E V-E-5 (Continued) Transect 1 d 6 d d 8 D C c d d 4 6 d 3,gge 3,gg 39,7 39,p 39,9 species 1974 1975 1976* 1977 1978* 1979 39,e g,gg 39,3 3,g3 3,gg Brook silverside 0.5 4.5 3.0 w Rock bass e White base Sunfish (Leposis) Green smfish 0.3 0.5 DD hybrid E 0.3 0.5 1.5 1.5 Cc 3.0 h t< Bluegill 6.6 1.5 3.0 0.5 1.5 0.9 1.5 1.5 1.5 Pumpkinseed Santish sp. 1.5 1.5 Smallm utn bass 0.9 2.3 3.0 0.3 0.5 4.6 3.0 3.8 4.5 9.0 3.0 1.5 5.0 3.0 3.0 = U IU Spotted bass 0.9 2.7 2.6 4.6 1.5 4.5 9.0 1.5 3.0 7.5 4.5 k t'* 1.5 0.9 3.0 Largemooth bass 1.1 1.0 1.0 0.9 4 Bass sp. 4.5 3.0 3.0 4.5 18.0 "M 1.5 hh 1.5

• 3 N

white croppie N Black etapple 1.5 1.5 l Johnny darter 0.5 hg l Banded darter 1,3

• 3 l

Yellow perch 8.3 0.5 0.8 3.0 [p l layperrb 0.3 0.5 1.5 3.0 Sauger 1.5 1.5 1.5 1.5 0.5 3.0 %k wolleye 3.0 3.0 1.5 1.3

• J Fressweter drum O

Unidentified h Total 150.8 645.2 139.4 235.9 65.6 250.6 146.9 225.2 176.0 418.5 241.5 67.5 670.5 304.5 361.5 142.0 % Y-JUL bggg, g CMAY-SEP,180V BAT. JUL, EEP ARD leDV ' MAT. JULY, SE? ARD DEC

1 i i w Tati.E V-E-5 (continued) transact 2a. 23. 3 I b e c 'ggygt .g,y,c iste 39,g - 1982 1993 2984 1995* - 1986 3,gy _1,gg 19,9 d e 0 0 0 d o d 6 ' Species 1974a 3,yg g,yg 3,77 longnose garl 0.5 Clarsta shed 'O.9' 2.0 1.4 0.7 8.3 2.1, 2.5 21.5 19.2 19.5 76.5 33.0 57.5 ~16.0 315.0 80.0 1 Tiger muskellenge 0.5 0.5 Meeke11ange 0.3 0.5 Worthern pike .0.3 0.2 Pike sp. 1.0. 1.0 ' O.S 0.' Coldfish 0,8 Cary 3.3 0.5 0.7 1.2 6.6 . 1.2 4.2 6.0 ' 4.8 3.0 20.2 10.0 - 9.5 5.0 6.0 5.5 mieer chub 0.5 Co15en shiner 0.2 0.5 0.3 0.5 Emerald shiner 67.7 239.9 '13.1 33.0 23.9 53.7 37.0 .163.5 21.8 493.5 . 22.5 21.5 36.5 31.0 13.0 7.0 w Striped shiner en Spotta11 shiner 0.5 3.5 1.0 Spotfin shiner 4.3 ' 2.0 6.1 4.9 0.5 0.5 1.0 0.0 1.0 4.0 1.5 2.0 0.5 0.5 g t;y Sand shiner 17.4 81.0 52.6 26.2 13.3 45.2 25.8 10.2 22.s 26.0 0.5 1.5 0.5 C s Minie shiner 1.8 1.1 0.3 2.2 1.0 3.2 4.0 7.0 1.5 0.5 CC Blantnose sinnov 6.1 31.2 45.3 4 4.9 ^ 21.4 40.0 10.2 5.2 14.2 38.5 0.5 1.0 0.5 0.5 0.5 > te ' creek chub C* tt3 ' 0.3 - g g*,, Stonarclier Blacknose doce . 0.2 h C' E Seiner op. 40.0 42.5 566.5 299.5 12.5 174.0 j Rieer carpeacker 1,3. gn Qu111 beck

• E 0.5 white sucker 0.5 0.3 0.1 0.3

+ 4.5 d-Northern hog socker 0.3 0.3 0.3 0.2 0.8 0.5 . O ' medhorse sp. 0.3 ~ 0.5 1.5 0.5 ' O.5 e $11eer redhorse 8.1 0.2 0.2 1.0 0.5 2.0 f( l Black redherse 0.3 0.3 Colden redhorse 0.0 0.2 1.5 1.5 1.0 2.0 0.5 0.5 2.0 ' g *< Shorthead redhorse 0.4 0.2 1.5 0.5 0.5 Yellow bullhead 0.4 0.2 0.2 [ l Erown bullhead 'O.4' D.2 0.1 c.1 0.5 W i Channel entfish 1.0 0.2 - 1.1 ' O.3 0.7 0.5 1.2 1.0 0.5 0.5 1.5 1.0

• 3 ~

Catfish sp. I 0.5 1.0 Trout-perch 0.1 0.5 0.2 0.2 5.0 Banded killifish 0.1 0.5 Brook sileerside ~ ~ 3.0 white base 0.1 0.5 15.0 10.5 L ar c6_ base 0.4 0.1 0.5 0.5 0.5 0.3 " mar-JUL DA0G. 907 ' E CnkT-SEF, NOV

• nnr. m. ser n o n w I
• nkr, M Y, an Ano Dec

=! I t i 1

TABLE V-E-5 (Continued) Transect 21, 2B, 3 8 b C 0 0 0 0 0 0 d d 6 Species 1974 1975 1976 1977e 397,e 1979' 1990 1991 1992 1983 1994 1985' 1986 1987 19,g 1999 p., em Sunfish (Leposis) w hybrid 0.3 0.2 $h Green sunfish 1.4 0.3 0.5 0.2 0.2 0.8 1.0 0.5 0.5 Pumpkinseed 0.! 0.7 1.0 0.5 0.2 0.2 1.0 0.5 ZO Bloegill 1.9 0.6 0.2 0.3 1.4 0.2 0.3 0.2 1.5 1.0 2.5 0.5 1.5 10 0.5 Sunfish sp. 0.5 0.5 0.5 hh' t* to Smallmouth bass 0.8 0.6 1.0 0.3 0.9 2.8 6.5 5.9 4.0 6.r 2.0 3.5 4.0 4.5 5.0 Spotted bass 0.4 2.7 2.1 1.5 0.5 0.8 2.5 9.5 1.0 2.5 7.5 5.5 4.0 Largemouth bass 1.4 1.1 0.7 0.7 0.3 0.2 0.0 0.5 2.5 0.5 < t* y Bass sp. 11.0 1.5 2.5 1.0 1.0 4.0 "y b= e White croppie 0.1 0.8 0.5 0.5 Bisek crappie 0.5 0.3 0.2 1.0 0.5 Johnny dartet 1.0 1.0 0.4 0.1 0.2 yH gn Banded datter 0.5 yQ -. Q Yellow perch c.1 0.2 0.2 0.5 Logperch 3.3 0.7 0.7 C.9 0.8 1.0 0.5 1.0 1.0 ta :p Sauger 0.5 0.2 1.0 0.5 1.5 w.5 Walleye h Freshwater drum 0.2 3.0 1.0 0.5 1.0 92 Unidentified 1.0 h Total 106.5 359.2 125.3 122.4 72.5 153.6 91.3 224.c 102.3 614.5 219.5 124.0 692.5 477.5 377.5 299.0 e 'MAY-JUL UACC, NOV

1. 14AY-SEF, NOV N JL% f"EP AND NOV

% r, m r. sEr n o CEC i V

~ DUQUESNE LIGIfT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT V-E-6), little change is noticed either between Control and Non-Control Transects or between preoperational and operational years. The 1989 gill net catch-per-unit-effort (fish /24 hours) is a high catch compared with previous collections with 3.1 and 5.1-5.9 f or the Control and Non-Control Transects respectively. Contributing to these yields are notably high catches of carp, channel catfish, and spotted bass. Comparison of ?reoperational and Operational Data Electrofishing and gill net data, expressed as catch-per-unit-effort, for the years 1974 through 1989 are presented in Tables V-E-5 ar d V-E-6. These sixteen years represent two preoperational years (1974 aad 1975) and fourteen operationel years (1976 through 1989). Fish data for Tran-sect 1 (Control Transect) and the averages of Transects 2A, 2D, and 3 (Non-Control Transects) are tabulated separately. These data indicate that new species are continuing to inhabit the study area and that, in general, the water quality of the Ohio River has steadily improved. Summary and Conclusions The fish community of the Ohio River in the vicinity of BVPS has been sampled from 1970 to present, using several types of gear-electrofish-

ing, 911',

netting, and periodically, minnow traps and seines. The results of these fish surveys _ show normal community structure based on species composition and relative abundance. In all the surveys since 1970, forage species were collected in the highest numbers. This indi-cates a normal fish community, since game species (precators) rely on 'this forage base for their survival. Variations in total annual catch are attributable primarily to fluctuations in the population size of the forage species. Forage species with high reproductive potentials fre-quently respond to changes in natural environmental factors (competition, food availability, cover, and water quality) with large changes in popu-lation size. These fluctuations are naturally occurring and take place in the vicinity of BVPS. 75 i

.w. s .e. s s e: 4 TABtz v-t-6 ' 7 CILL HET CATCB (TigW24 BOW) MrJIIS (I) AT 2manSECTS IN TEC Mbr CUMBER 1AND POOL ' a

TEE OE70 RITER,1974-1999 -

svPS 't. Transect 1 6 g,7,6 g,7,4 g,gge . g,gge g,gge g,g3e . 3,,ge. g,,g t 1996* 1987' . g' 1999'

1975b, y,ge

.g,77 Species 1914' ' C.2 ' 0.3 T.. Inngnose gar 0.1 0.1 0.1 0.4 0.1 0.1 Cissard shad %,y, mainbow trout 0.1 .[ soorthern pike 0.1 Nokellunge 0.1 0.1 0.3 0.1 Tiger muskellunge 0.1 0.1 Gbid fish 1, Crass carp 0.I' e.* 0.5 0.2 0.3 0.4 0.4 2.4 ' 1.0 90 Carp c.4 1.2 0.1 0.4 0.5 0.1 0.4 ,e,, Goldfish a Carp hybr id {D Rieer carpsacker 0.1 C.1 Quillback 0.1 0.2 ~ 0.1 0.1 lE 80 1 -0.3 O.2. 0.2 CC White sucker [Q~ 0.1 Black redhorse 0.1 0.1 2 Sileer rednorse Golden redherse 0.1 0.1 0.1 0.3 0.3 '@M-7 'f shorthead redboree 0.1 0.3 g. M M 4 medacree sp. .ge i m slaca bullhead arown bullhead 0.4 0.1 y Yellow bullhead-0.1 White catfish O Channel catfi.ih '0.9 0.7 0.7 0.2 0.2 0.2 : 0.4 0.2 0.4 0.6 0.4 0.4 0.1 H' j Flathead catfish 0.1 [p j Mbite bass 0.2 0.5 0.1 - Z-0.1 ..% f Rock bass 0.3 C.2 0.1" 0.2 .i 0.1 0.1 l-Green sunflah o. Pumpkinseed 0.1 O .[ sluesill 6 ,l Smallmsuth bass 0.1 0.1 0.1 t largencasth bees 0.2 0.1 0.1-0.1 t I Spotted bass 0.2 0.7 0.1 0.1 0.5 1.5 ' 1.0 0.4 0.1 1.3 'r9 0.1 0.1 ( White crapple 0.1 l. Black crappie [ 0.1 Yellow perch 0.4 0.6 0.$ ' O.8 0.3 0.2 0.1 0.1 walleye 0.2 0.3 0.3 0.3 0.2 0.1 0.4 0.5 0.3 0.3 0.1 0.1 0.2 0.1 l Souger 0.2 Freenweter drum = = = 0.2 0.2 0.1 I Total 1.8 3.4 2.2 3.2 2.9 0.0-1.3 0.4 0.5 2.4 4.2 0.6 2.7 2.0 1.5 6.0 3.1 [ t 3 h 1 .i b. 4 4 s

.-e .7 k ] r -1 TAsLE V-> 6-Jscoattnerev ,7 Treasset 2&, E A -Species 1974a 'g,7$b ~ d d g,y,d - 39,,e 3,g ge 1902' ,19 13', 1984' 1935 - 19e(' 1941*. _193g' - 1939e g,yg g,97 3,9g f e <0.1 < D.), <0.1 ' - 0. A 1mnynoop gar < 0.1 < 0.1 0.7 0.1 0.4 0.8 0.1 0.3. c.1~ k , 0.1 Gissard shed 0.2 -- 0.1 8.1 mnoneye. < c.1 - . mainbow trout -~ i northern pike 0.1 < 0.1 <0.1 <0.1 < 0.1 < 0.1 - < 0.1 < 0.1 < 0.1. 3.1 < g.1 0.2 sesskellueure 'O.1 T19er muskellerspe. < 0.1 < 0.1 <0.1 < 0.- 1 '0.1 0.1 ~. < 0.1 0.2 0.3-asidfish ~ . Carp 0.9 0.3 0.2 0.6 0.3 0.3 0.2 - ' O.3. 0.9 0.9 0.3 0.5 1.0 0.4 2.1 ' 1.3 ' Goldfish a Carp t bybrid c.1 0.1 River corpsacker = = < 0.1 S.1 0.1 0.2 <0.1. 'p. Ou111 beck < 0.1 0.2 0.1 <0.1 d'O.1 < 0.1 0.2 0.1 0.1 <0.1 w mite sucker. c.1 - < 0.1 < 0.1 < 0.1 - 0.1 < 0.1 < 0.1 < 0.1, smallmouth buff alo - <0.1- < 6.1 0.1 < c.1 gg Black redhor se - Silver rednorse < 0.1 0.2 0.1 < 0.1 < c.1 < 0.1 z sO - ') Q)16en redborne l- <0.1 <0.1 0.2 0.1 0.2 0.2 0.? C C' + <0.1 < 0.1 < 0.1 f Q. Shorthead redboree < 0.1 <0.1 0.1 <0.1 0.1 col medhor s<r op. z j 1 Black bullhead 0.1 g 29 l Erawn bu11 bead 0.2 < 0.1 < 0.1 4g. .a fellow bullhead 0.1 ~ < 0.1

• • a.*

gO -J mite catfish < 0.1 l' channel entfish . 0.3 1.3 0.4 1.0 0.4 0.5 0.4 0.6 0.7 0.5 0.3 - 0.s 1.1 0.5 0.7 1.2 3 Flathead catfish < 0.1 < 0.1 <0.1 < 0.1 0.1 0.1 0.1 (0.1 S.1 0.8 0.3 i m ite bees 0.1 Rock bass 0.1 < 0.1 < 0.1 < 0.1. < 0.1 0.1 < 0.1 0.2 <0.1 0.2 < 0.1 < 0.1

• 9 0.1

< 0.1 %N l creen sunfish 0.1 < 0.1 Peopkinseed Bluegill 0.1 < 0.1 <0.1 < 0.1 < 0.1 g'< <0.1 <0.1 r ( smallmouth bass .n ' Largemouth base 0.2 0.1 0.1 < 0.1 < 0.1. < 0.1 < 0.2 < 0.1 O [ j Spotted base 0.2 0.1 < 0.1 <0.1 0.1 < 0.1 0.3 1.0 0.2 0.5 ' O.1 0.7 2.2 1.0 < 0.1 < 0.1 0.1 0.1 < 0.1 0.2 0.2 C.1 < 0.1 < 0.1 . mite crappie alack crappie < 0.1 0.1 < 0.1 i,.1 (0.1 0.1 < 0.1 <0.1 Yellow perch 0.7 0.5 0.7 'O.1 - 0.1 (0.1 < 0.1 < 0.1 <0.1 umileye e.2 0.2 0.1 0.2. - 0.1 <0.1 0.2 0.1 0.7 0.1 0.1 0.1 < 0.1 4.2 e.1 i 0.1 < 0.1 0.2 0.3 <0.1 0.2 0.3-0.5 0.a 0.2 - 0.3 0.2 0.7 0.; sanger f IPreenwater drum 8.1 0.3 0.2 < c.1 0.2 0.1 Total 2.2 ' 3.1 1.5-2.2 3.6-4.3 1.3-1.9 1.3-1.9 1.2-1.6 1.5 4.4 5.2 2.0 3.3-4.0 3.e-4.s

2. b 3.1 s.1-3.7 3.1-5.9 I

r 1

.Su.

• nu-ser, e bADG. SEF.107

'MfLY,.ML. SEF, 3I07 8 'I 35LT-SEP M&I, JUL, SEP, DEC I i t i s k i l t k .-ww n +- .. + - -

l DUQUESNE LIGHT COMPANY l 1989 ANNUAL ENVIRONMENTAL REPORT Although variation in total catcl. has occurred, species comprisition has remained f airly stable. Since the initiation of studies in 1970, forage fish have dominated the catches. Carp, channel catfish, smallmouth and spotted bass, and walleye have all remained common species. Since 1978, aauger have become a common game species near DVPS. bifferences in the 1989 electrofishing and gill net catches, between the Control and Non-Control Transects were similar to previous years (both operational arm preoperational) and were probably caused by habitat pref-crences of individual species. This habitat preference is probably the most influential factor that affects where the different species of fish are collected and in what relative abundance. Data collected from 1970 through 1989 indicate that fish in the vicinity of the power plant have not been adversely affected by DVPS operation. F. .. JTHYOPLANKTON s 4 Objective Ichthyoplankton sampling was performed in order to monitor the extent fishes utilize the back channel of Phillis Island as spawning and nursery grounds. Methods The 1989 program had five day surveys (April 13, May 23, June 19, July 12 and August 15) and two night st.t veys (May 24, and Jnly 13) conducted during the spring and summer, which is the primary spawning season for most resident fish species. One surf ace and one bottom collection were taken at Transect 2B (back channel of Phillis Island) during each survey (Figure V-F-1). Tows were made in a zig-zag fashion across the channel utilizing a conical 505 micron mesh plankton net with a 0.5 m mouth dia-meter. l l 78 s

t1 Og2(MIDLA %,AE ( w 9 %* seem 'Y g MANSFIELD ~ o wgg+y?: i o l 1, qe !!n 45 E d 2a ' -

• \\

8s u H A SURFAG TOWS O D1 BEAVER VALLEY DISCHARGE e ;,***aa. g @6 D2 INDUSTRIAL DISCHARGE i g O NN 3 e AID 'to NAVIGATION

• 4628 l

v i BEAVER 4 TRANSMISSION LINE "m (; -. M," rowEn STATION Q [ e FIGURE V-F-1 ICHTHYOPLANKTON SAMPLING STATIONS BVPS

__.-_______..-_.s-_.. ~ ' DUQUESNE LIGiff COMPANY = 1989 ANNUAL ENVZRONMENTAL REPOYt ' A ~ Ceneral. Oceanics Model 2030 digital flowmeter, mounted centrically in the -net south, was used to determine the volume of water filtered. Sam-i ples were : preserved in the field using 54 buffered formalin containing _ rose _ bengal-dye. In the laboratory, ichthyoplankton was sorted from the sample and enu-merated. Each specimen was identified as to its stage of development --(egg, yolk-sac larvae, early larvae, juvenile, or adult) and to the low-3 est_possible taxon. Densities of ichthyoplankton (numbers /100 m ) were . calculated for_each sample using flowmeter data. Results A total of 1,586 eggs and 621 larvae were collected in 1989 from 1,659.3 3 m of water sampled - (Table V-F-1). Nine taxa representing six families -- were - identi fied. Gizzard shad (Dorosoma cepedianum) accounted for 16.3% of the total catch.= Preshwater drum eggs (Aplodinotus grunniens) repre-sented ' 99.2% of the eggs collected in 1989. For 1989, the night collec-3 . tions produced a total' density of 381.60 individuals per 100 m compared 3 rea those from day collections which were 21.72 individuals per 100 m. Of the' day collections,~ fensities on July 12 were most abundant with a 3 total' density of 96.60 individuals per 100 m (mostly gizzard shad lar-vae). The most abundant densities for the night colle::tions were on July 13_with a total density of-707.82 individuals-per 100 m3 (freshwater drum eggs and larvae, and gizzard shad larvae). No ichthyoplankton were col ' lected in. April (Table V-F-1), whereas -- total catch in May yielded two eggs:and three larvae. . Comparison of Preoperational and Operational Data Species abundance and composition.were similar to that-found in previous (7 years. Gizzard shad, and ' f reshwater - drum dominated the _ catch and other l_- . taxa. were represented" by only a few individuals. Densities of ichthyo-plankton - collected in the backchannel (Station 28) from 1973-1974, 1976-1989,_are-presented in Table V-F-2. 80

MELE V-F-1 NtMBER AlO DENS {TY OF FISE BOGS, LAPVhE, JUVENILES, A10 ADULTS (Number /100 m ). CDILECTED WITH A 0.5 m FMFK10N NET IN THE OEIO RIVER BACK CBAletEL OF PEILLIS ISIAND '(STATION 25) NEAR svFS, 1989 Depth of Collection Date Surface Bottom ' Total Collection and April 13 ~g Night M Might Tara Density 3 vol. water filtered (m ) 137.3 153.6-290.9 Number eggs collected 0 0 0 Number larvae collected 0 0 0-Number-juveniles collected .0 0 0 Number adults collected 0-0 0 Density (number collected) 0 0 0 Total Density -trunnber collected) 0 0 0 em-May 23/24 4-DD 3 Eb vol. water filtered (m ) 166.7 128.8 147.1 108.1 550.7 Number eggs collected '0 1 1. 0 2 5' Number larvae collected 0 1 1 1 3 Number juveniles collected .O 0 0 0 0 g tJ. Number adults collected D

• 0 0

0 0 < ta ' Density (number collected) E Eggs Unidentified egg (EE) 0 0.78(1) 0.68(1) 0 0.36(2) 'I Larvae EO Stizostedion opp. 0 0.78(1) 0.68(1) 0.93(1) 0.55(3) y l Total Density (number collected) 0 1.55 (2) 1.36(2) 0.93(1) 0.91(5) ta g gN r June 19 O Vol. water filtered '61.1 48.7 109.E N 'T Number m s collected 0 11 11 [ Number larvae collected.. 5 12' 17 i Number juveniles collected O. 0 0 Number adults collected 0 0 0 Density-(number collected) Eggs Cyprinus carpio (EE) 0 . 10.27(5) 4.55(5) Unidentified egg (EE) 0 12.32(6) 5.46 (6) Larva _Dorosoma cepedianum (EL) 1.64(1) 0 0.91(1) Cyprinus carpio (YL) 0 4.11 (2) 1.82(2) cyprinus carpio (EL) 4.91(3) 12.32(6) 8.20(9) Catostomidae (EL) 0 6.16 (3) - 2.73(3) Etheostoma spp. (EL)- 0 2.05(1) 0.91(1). Unidentifinble (*L) 1.64(1) 0. 0.91(1) Total Density (number t.ollected) 8.18(5) 47.23(23) 25.50(28) l

6- .o-X maI2 V-F-1 (Corstinued) Depth of Cb11ection Date surface Botton letal Collection and Dy Night 'M Might Taxa Density July 12/13 3 vol. water ' filtered (m ) 136.8 144.7 '80.6 ~131.5 493.6 Number eggs collected. 11 780 2 778 1571 Humber larvae collected 129 215 68 182 594 Number juveniles collected 0 0 0 0 0 i Number adults etallected 0 ~ 0 0 0 0 . Density (number collected) Eggs Aplodinotus grunnient (EE) 8.04(11) 539.05(780) 2.48(2) 591.63(778) 318.27(1571) Larvae.. $E -Cyprinidae (YL) 1.46(2) 1.38(2) 0 3.04(4) 1.62(8) Dorosoma cepedianum (YL) 0.73(1) 6.91(10) 3.72(31-1.52(2) 3.24(16) p g' Dorosoma cepedianum (EL) '74.56(102) 87.77(127) 63.28(51) 47.15 (62) 69.29(342) z cyprinus carpio (EL) 0.73(1) 7.60(11) 1.24(1) 0.'4 (1) 2.84 (14) @M Notropis spp. (EL) 6.58(9) 0.69(1) 3.72(3) 2.28(3) 3.24(16) '$[_ Pinephales spp. (EL) 2.92(4) 0.69(1) 0 0 1.01(5) g *9 Pomoxis app. (EL) 0 0 0 0.76(1) 0.20(1) ~3 Aplodinotus grunniens (YL) - 6.58(9) 38.70(56) 7.44(6) 74.52(96) 34.24(169) n - Aplodinotus grunniens (EL) 0 2.76 (4) 0 6.84(9) 2.63(13) ~Q Dnicentifiable ('L)- 0.73(1) 2.07 (3)

4. 96 (4) 1.52(2) 2.03(10)

N 83 Total Density (number collectedt 102.34(140) 687.63(995) 86.85(70). 730.04(960) 438.61(2,165) C* E ' N August 15 g

o 3

vol. water filtered (m ) 110.0 104.3 214.3

• 8 f

Number eggs collected 0 2 2 Number. larvae collected 0 7 7 Humber juveniles collected 0 0 0 Humber adults collected 0 0 0 Densities (number collected) . Eggs Aplod.notus grunniens (EE) 0 1.92(2) 0.93(2) -Larvae. I Aplodinotus grunniens (YL) 0 4.79(5) 2.33(5) Aplodinotus grunniens (EL) 0 3.92(2) D.93 (2) Total cusity (number collected) 0 8.63(9) 4.20(9)

TABLE V-F-1 (Continued) Depth of Collection Surface Bottom 'Ibtal Collection and Yearly Tot f Dy Ni@t M Wi@t Taxa Density 3 3 Vol. water filtered (m ) 611.9' 273.5 534.3 239.6 1,659.3 Number eggs collected 11 781 16 778 1,586 Number larvae collected 134 216 88 183 6 21 Number iuveniles collected 0-0 0 0 0 Mumber adults collected 0 0 0 0 0 Densities (number collected) i Eggs t Cyprinus crecio (EE) 0 0 0.94(5) 0

v. O f5 )

U Aplodinotus grunniens (EE) 1.80(11) 285.19(780) 0.75 (4) 324.71(778) 94.80(1,573) g Unidentified egg (dE) 0 0.37(1) 1.31(7) 0 0.48 (8) - Larvae $8 Dorosome cepedianum (YL) 0.16 (1) 3.66(10) 0.56(3) 0.83(2) 0.96 (36) y@ ? Dorosoma cepedianum (EL) 16.83(103) 46.44 (127) 9.55 (51) 25.88(62) 20.67(343) g en cyprinidae (TL) 0.33(2) 0.73(2) 0 1.67(4) 0.48(8) Cyprinus carpio (TL) 0 0 0.3Jt2) 0 0.12(2) Q 89 Cyprinus carpio (EL) 0.65(4) 4.02(11) 1.31(7) 0.42(1) 1.39(23) $[ i w Notropis opp. (EL) 1.47(9) 0.37(1) 0.56(3) 1.25 (3) 0.96 (16) o g$ Pimephales opp. (EL) 0.65(4) 0.37(1) 0 0 0.30(5) ~fn ,Pomoxis opp. - (EL) 0 0 0 0.42(1) 0.06 (1) i Catostomidae (EL) 0 0 0.56(3) 0 0.18(3) g. Etheostoma spp. (FL) 0 0 0.19 (1) 0 0.06 (1) tg Stizostedion opp. ~ %) 0 0.37(1) 0.19(1) 0.42(1) 0.18(3) Aplodinotur grunniens (TL) 1.47 (7 ~- 20.48(56) 2.06 (11) 40.90(98) 10.49(174) g *< aplodinotus grunniens (EL) 0 1.45(4) 0.37(2) 3.7b(9) 0.9 0(15 ) g Unidentifiable (*L) 0.33(2) 1.10(3) 0.75 (4) 0.83(2) ' 0.66 (11) y H Total Density (number collected) 23.70(145) 364.53(997) 19.46(104) 401.09(961) 133.01(2,207) " Developmental Stages YL - Batched specicens with yolk ar.J/or oil globules present. EL - Specimens with no yolk and/or oil globules and with no development of fin rays and/or spiny elements. LL - specimens with developed fin rays and/or spring elements and evidence of a *in fold.

• L

. specimens with undefinable larval stage due to deterioration. JJ - Specimens with eceplete fin and pigment developteent, i.e., issnature adult. o g

.~-- DUQUESNE LIGilT COMPl.NY .98) ANNUAL ENVIRONMENTAL REPORT TA B;J ' V-F-7 3 DENSITY OF ICllTifYOPLANK't04 (Number /100m ) COLLBCTED IN 11tB Oll!O RIVER IMCK CilANNEL OF Pil!LLIS ISLAND (STATION 18) NEAR DVP3,1973-1974,1976-1989 Date p>g s ity, Date_ De nsitis Date Dene l,(g 1973 1974 1976 Apr 12 0 Apr 16 0 Apr 26 0.70 May 17 0 lity 24 0 May 19 0 Jun 20 16.10 Jun 13 6.98 Jun 18 5.99 Jul 26 3.25 Jure 26 9.25 Jul 2 6.63 Jul 16 59.59 Jul 15 3.69 Aug 1 6.83 Jul 29 4.05 1977 J 7,8_ 1979 Apr 14 0 Apr 22 0 Apr 19 0 May 11 0.90 hay 5 0 May 1 0 Jun 9 24.22 May 20 0.98 May 17 0.01 Jun 22 3.44 Jun 2 4.01 Jun 7 0.39 Jul 7 3.31 Jun 16 12.15 Jun 20 11.69 Jul 20 28.37 Jul 2 13.32 Jul 5 14.82 1980 1981 IT-9 2 Apr 2.* 0.42 Apr 20 1.10 Apr 19 0 May 21 0.53 May 12 0 May 10 3.77 Jun 19

9. ( B Jun 17

26. 10 Jun 21 7.54 Jul 22 107.04 Jul 22 17.14 Jul 20 31.66 1983 1984

_1985 Apr 13 0 la ' 16 0 Apr 18 0 May 11 0.66 May 10 0 May 14 1.81 Jun 14 4.46 Jun 8 15.46 Jun 10 13.36 Jul 12 44.05 Jul 12 44.23 Jul 11 117.59 1986. 1987 1986 Apr 18 0.63 Apr 21 0 Apr 18 0 May 13" 5.93 Hey 19 16.22

tay 10 0.42 8

8 Jun 19 34.52 Jun 19 40.02 Jun 14 162.43 Jul 15" J 6.15 Jul 14 19.25 Jul 14a 39,4g 4 Aug 12 9.89 Aug 10 7.87 Aug 16 1.32 1989 Apr 13 0 May 23' O.91 Jun 19 25.50 Jul 12a 438.61 Aug 15 4.20 " Day and night survey was conducted. 84

__._..m._.___.._ ._._ _ _.._ _.. _. m. _ ~.. _ _ _. DUQUP...'t LIGIFF COMPANY 1989 ANNUAL anf!RONMENTAL REPORT t Summary and.anclusions 01 :ard shad, and freshwater drum dominated the 1989 Achthyoplankton catch from the back channel of Phillis Island. Peak denalties occurred in July and consisted mostly o( eggs. No spawning was noted in April. The month of May showed little spawning activity. There was a decrease in larvae dent'ity af ter July. G. FISH IMPINGEMENT e t Objective, Impingement surveys were conducted to monitor the quantity of fish, other - aquatic ~ organisms and Corbicula lapinged on the traveling screens.

Methods, The surveys were conducted weekly throughout 1989 for a total of 40 weeks (Table-V-A-1).

Except when technical difficultles delayed the start of collections, weekly fish ' impingement sampilng began on Thursday mornings when all operating screens were i< ashed. A collection basket of 0.25 inch mesh netting was placed at the end of the screen washwater sluiceway (Figure V-G-1). On Friday mornings, af ter approximately 24 hours, 'each acreen was washed individually for 15 minutes (one complete revolution of the screen) and all aquatic organisms collected. Fish' were identified,. j counted,- measured for total langth (mm), and weighed (g). Data were i. (< summarized according to operating-intake bays (bays that had pumps oper-i ating'in the 24 hour sampling period). and non-operating intake bays. [ i.. Results l L The BVPS impingement surveys of 1976 throt.,.i 1989 have resulted in the collection of 39 species-of fish representing ten families (Table. + V-G-1). A ' tatal of 550 fish, representing 14 species were collected in [ 1989 (Table V-G-2). 85 er v-- v--rww,- rw ,w+- .,e~+.cw. w,y,,3% .y.7,y.--y., ,n ..7e- ,,.m y 7 -w--.r.vu,-+r=.g-rr'-*'-vr"*F

DUQUESNE LICitT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT aut wisieusonesnan ene us senu.nu 7 satt tests (at e n.i n io... n ns * " 4 /- 'unn n.un ons in n,....... nnu.in innin a enni n.....,, / Elh h f > fllll'!.!'li!!!l'llllin 1 T w .I [ [ eninaanen.non!!'lli !!'.""'" "" g / .y >Q ~- s \\ 3{, 1 s tj1 u en i n...., 2 f}L f G x. u su * " N q fij;j,g,, T 1L L j M sia as not i os

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,,,,o o n n,,. (Three dimensional: Cutaway view) o e FPODAP4.8 flAS FL. 730.0' stevit's Stare SL. 190.9' g untra tener DitABL #888 g Ti m a;" r,,,,,, n/, ri........ a (-- ( eut stor p l # LMcER If f I.. .9* w [. na s l t t.. 664.l' ' IfI _ eg. Si,.g. EITEFF98 UW [ WAftA BL, 640.$4 ^ r...... g \\........ ti (Two dimensional: Side View) FIGURE V-G-1 l [ INTAKE STRUCTURE l DVPS t L 86 l. i.

DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT 4 l TABLE V-G-1 i l FISil COLLECTED DURING Tile 1MP!NGEMENT SURVEYS, 1976-1989 l BVPS i l ' Family and Scientific Name Common Name Clupeidae (herrings) Dorosoma cepedianum Gizzard shad E Cyprinidae (minnows and carps) Cyprinus carplo Common carp Hybosnis storerlana S13ver chub Notegigonus crysoleucas Golden shiner l -Notropia atherinoides Emerald shiner N. hudsonius-Spottall shiner N,. spilopterus Spotfin shiner N. straminous Sand shiner N,. volucallus Mimic shiper Pimephales notatus Bluritnose minnow senotiles atronaculatus Creek chub l Catostomidae (suckers). -Carplodes cyprinus Qu'11back Catostomus commersoni White sucker 1 Nonostoma carinatum River redhorse Ictaluridae (bullhead and catfishes) i Ictalurus catus White catfish I_. natalis Yellow bullhead J_. - ne bulosus. Brown bullhead J,. punctatus. _ Channel catfish i Noturus flavus Stonecat M odictIs olivaris Flathead catfish Percopsidae ' (trout-perches) Percopsia,omiscomaycus Trout-perch 4 Cyprinodontidae _ (kix *ishes) Fundulus diaphanus Banded killifish Percichthyldne -(temperate basses) _Morone chrysops White bass W 87 . m--

.. -. _- ~ _ - ___ -... __.~... DUQUESitt LIGilT COMPAliY 1989 AtittUAL DIV!l014HLiiTAL REPORT 1 l TABLE V-G-1 i (Continued) l Family and scientific flame Common fiamo Centrarchfidae (aunfishes) Amblopliten tupostris Rock basa Lepomia, cyane11us. Gteen sunt18h L. gibbosuo Pumpkinseed L,. macrochirus Bluegill Micropterus dolomieu! Smallmouth bass M. punctulatus Spotted bass M. salmoides Largemouth bass Pomoxis annularls_ White crapple P, nigromaculatus Black crapple Percidae (perches) Etheostoma nigrum Johnny darter E. tonale Banded dartet Perca _flavenscens Yellow perch Percina caprodes Logperch P. copelandi Channel darter Stizostedion vitreum vitreum Walleye Sciaenidae (drums) Aplodinotus,grunniens Freshwater drum 1Nomenclature follows Robins et al. (198f)) t i o 88 1

i ? i t l j i I r Ei f tassa v-4-2 Sutomar or riss NW Zu Istim SUETITS COIBFX"TED hm 014: 24 EDUR PERIOD Pts must agafsc 1989 NTFS I 2 5 C7tm TING IWThEE SAT 5 REB-OPrahTTWC TWTApr SATS Percent ut se Deed Mase Deed f.ength e Fregeeney kreent usaget meaght. m aget wetget Range 3- . occurrence Caesosition ,Ng (et

1. mmer (et museer ett museer to fast e

i Teen puomet j.- C1:serd obed 437. 23 79.5 428 2352 9 52 46-250 ZCC Emerald shiner 1 ~ 3 0.2 1 1 35 3 channel catfish 5 15 1.1 3 18 2 21 1 4 62-138 rietheed catfien' 12 8 2.2 4 4 8-t 38-59 g es i White boos' 1' 3 0.2 1 35 195 4p moet bees 11 23 2.0 3 10 1 2 5 a 2 2 33-70 Green sunfish 5 15 1.1 4 31 1 17 1 4 60-97 gQ f Pumpkinseed 1 3 0.2 ' 1 10 77 3 4 Bluegill 13 28 2.4 4 9 6 13 1 1 2 3 36-71 g Smelleonth bees 2 . 5 4.4 1 148 1 62 173-220 m i Spotted base 7 15 1.3 4 151 1 205 2 23 102-245 y i white crapple 5 13 0.9 1 2 4 14 53-33

g. g i-Logperch 2

5 0.4 1 4 1 3 31-82 + Freshweter drue 44 30 8.0 4 17 39 176 1 2 1 4 32-123 Unidentifiable 2 5 0.4 2 45 100-130 TO 1 Str j Total 350 30 444 494 3a*" 11 55 15 55 +9 j ' 1 Intake boys that.hed pape operating within the 24 hour sempling period. j 2 Intate boys that had no peeps operating within the 24 hour smopling period. 1 4- } i 4-i i I 1 g

l DUQUESi4E LIGi!T COHPA11Y 1989 AlltiUAL DWIROliHD4 TAI. REPORT Gizzard shad were the most numerous fish, comprisir.g 79.5% of the total annual catch, followed by f reshwater drum (8.0%), bluegill (2.4%), with all other species r epr.s se nt ed by less than 13 specimens. All fishes ranged in size from 32 mm to 260 mm, with the majority under 100 mm. The total weight of all fishes collected in 1989 was 4.05 kg (8.9 lbs). Approximately 71.6% of the total weight of fish collected (both alive and dead) was comprised of gizzard t.had collected in December. llo endangered ,r threatened species were collected (Commonweal th of pennsylvania, 1985). The temporal distribution of the 1989 impingement catch closely follows the pattern of catches of previous years (1976 to 1988) (Tables V-G-3 and V-G-4 ). During each year, generally th e largest numbers of fish have been collected in the winter months (December-rebr uar y) and then the catch has gradually decreased until the late summer period when another, smaller peak has occurred. Other organisms collected in the impingement surveys include 94 crayfish, 137 native clams, and 47 dragonflies (Tables V-G-6 and V-G-0). In addi-tion, 16,577 Asiatic clams (Corbiculs) were collected (Table V-G-7). Comparison of Impinged and River Fish A comparison of the numbers of fish collected in the river and traveling screens is presented in Table V-G-5. Of the 33 species collected, 12 were observed in both locations, two species were collected only in the impingement surveys, while 19 species were taken exclusively in the C river. The major difference in species composition between the two types of collections is the absence of large species in the impingement col-1ections. Seven species of nuckers (river carpsucker, qu111back, white aucker, smallmouth buf f alo, silver redhorse, shorthead redhorse, golden redhorse) and five species of game fish (muskellung e, tiger muskellunge, black crappie, walleye, and sauger) were collected in the river studies, but were not collected in the impingement surveys. Ga.ac fish which were collected on the traveling screens (channel catfish, rock bass, and blue-gill) were smaller than individuals of those species collected by river sampling. i 90

i [ T l I E Tamb.i: T-G-3 ' i sussuutr Or twInceaurr suwar am ran 19e9 4 i 2TUS i River Operating lean-operatigg Intake Boys intate 21eestion I l Date Isesem: of Fish Percent 2ntake mere Intake Boys' operating water Above semen I f Ptonti _g Collected Anneel Total A12*e Dead Alive Deed A B, C D Tear *P 'See Level (ft.) i Jennery. 6 329 59.8 2 327 x x x 27.2 646.0 t 13 - '23-4.2 1 22 x x x x 37.0 667.5 h ' 20 26'- 4.7. 2 24 x x x x 41.2 646.2 i. ' 27 13 2.4 '9 1 3 x x x 40.1 666.8 t 7ebruary 3 8 L5 2 5 1 X X X 43.2 646.1 10 27 4.9 3 24 x x x x 27.s 665.5 I 17:33 I 38.9 672.6 l 39.4 66,.. gg [ 32 24 i EO T .. Pterch 3 54 9.8 53 1 x x x 34.3 665.4 5 y$ { 4 10 14 2.5 2 7. 5 I 2 x 41.8 667.6 t* g 17 6 1.1 1 5 x x 'x 47.0 646.2 i gn f j 24 4 0.7 2 1 1 x x 46.0 647.8

4. g, 54.0 674.9 L

f e 31 gO [ 1 w E [ 4 April 7 3 0.5 2 1 x x 49.8 671.1 gH j 14 2 0.4 2 x x x 48.4 667.7 g r) j 21 2 0.4 2 'I X X 55.0 666.2 yQ 28 1: 0.2 1 x x 59.0 465.8 >E ta > l Z May 5 0. 0.0 x x I 58.8 666.8 gM 12 2 0.4 1 1 x x x 51.6 675.5 =p 57.2 670.9 I 1? 61.0 667.0 6 j 26 I ? June 2-1 0.2 1 x x x 71.2 666.3 I f 5 0 0.0 x x x x 72.2 665.5 [ 16 0 0.0 x x x 68.0 672.0 t 23 0 0.0 x x x x 66.3 672 0 t 30 1 0.2 1 x x x x 71.3 668.2 (i L 4 l t i [. i

J-o b l I l I 1 i 4 TASIJ: V-G-3 i i (Contimand) r i Operating non-operatigg Intake Says Intake Elevatiore 1 Date Number of Fish -Percent ' Intake Seys t_ptabe nors' Operatim; Water Aboee steen f nonth Dey en11eeted Annual Total Alive Deed Altwe Lead A B C D Temp *r see z,e,,1 fft.) 5 t July' 7 3 0.5 2 1 2. I x x 75.0 646,9 [ l 14 1 0.2 1 1 1 x x 79.0 665.s i 21 0 0.0 x x x 78.4 666.2 I j 2e 1 c.2 '1' x x x x s2.1 665.3 }. August 4 0 0'.0 i x x x x 79.2 665.9 y 11 0 0.0 1 2 1 1

7. 5 m.1 e

is 6 1.1 1 5 I 2 x x 7s.9 665.0 i 25 6 1.1 3 3 x x x x 79.s 666.3 gg [ { zo September 1 1 0.2 1 x x x x 79.0 666.0 CC 6 s 0 0.0 x x x 77.0 665.3 E Ei 15 2 0.4 2 2 x 76.1 665.s g 22 0 0.0 x x 73.6 665.8 I 29 4

0. 7 '

4 x x 66.8 665.6 NC f E ge October 6 2 0.4 2 x x x 66.0 665.4 ~4 [ ho 13 0-0.0 x x x 6c.3 465.9 i 20 2 0.4 2 x x x 60.2 667.2 ~Q l 27 3 - 0.5 1 1 1 2 2 x 56.0 665.s y% I t- > k [ I8I November 3 56.6 565.7 1: 10 I St.c 666.0 I 17II 50.5 667.3 O i 24 2 0.4 2 x x x 42.6 665.9 E December II8I 43.5 665.2 s (5I 3s.0 666.0 ( 15(5) 35.6 '665.7 I 22(5) 33.2 665.4 i 1 29 1 0.2 ~ 1 2 x x 33.7 665.7 i Total 550. 30 494 11 15 1 Intake boys that had pg operating in the 24 hour sampling period. 2 Intake' boys that bed no pumps operating in the 24 boer sampling period. 38 ' Impingement could not be conducted due to high water conditions. Impingement could not be conducted due to diving operations in screenbooes. [ 5 Impingement could not be conducted due to maintanence. I 3 w ~ ,or y ,.,-..,._w - - - - - -.,,. - - +.. --w..

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22,.., a ~ 57 t-II l i I' .i d d II' S It f 94 -. ~,. - -.. - - -

DUQUESNE LIGil? COMPANY 1989 ANNUAL DWIRONMENTAL REPORT TABLE V-G-5 NUMBER AND PEICENT OF ANNUAL TOTAL OP FISil COLLECTED IN IMPINGTRDiT SURVEYS AND IN Ti!E NEW CUMBER 11diD POOL OF TIIE 01110 RIVER,1989 DVPS Total Number of Percent of Fish Collected Annual Total Species (a) g ingement River Impingement-River Longnose car 5 1.0 Gizzard shad 437 222 79.7 45.4 Muskellunge 2 0.4 Tiger muskellunge 9 1.8 Grass carp 1 0.2 Common carp 55 11.2 Golden shiner 1 0.2 Emerald shiner 1 23 0.2 4.7 Spottail shiner 3 0.6 Spotfin shiner 1 0.2 - River carpsucker 5 1.0 Quillback-2 0.4 White sucker 1 0.2 Smallmouth buffalo 1 0.2 ' Silver redhorse 2 0.4 Golden redhorse 15 3.1 Shorthead redhorse 2 0.4 l Channel catfish '6 30 1.1 6.1 Flathead' catfish 12 ._2 2.2 0.4 White bass 1 31 0.2 6.3 Rock bass. 11 5 2.0 1.0 Green sunfish 6 1.1 Pumpkinseed i 1 0.2 0.2 Bluegill 13 1 2.4 0.2 Smallmouth bass 2 12 0.4-1.5 Spotted bass 7 39, 1.3 8.0 White crappie. 5 1-0.9 0.2 Slack crappie il 0.2 Banded darter-1 0.2 Logperch' 2 0.4 i. -Sauger 7 1.4 Walleye 3 0.6 Freshwater drum 44 5 8.0 1.0-l l Total' 548 489 t I"I Includes only those specimens identified to species or stocked hybrids. I l; I 95 . =,.

.. ~ DUQUESNE LIGitT COMPANY 1989 ANNUAL D4VIHOllMDITAL REPORT TABLE V-G-6

SUMMARY

OF CRAYFISH COLLECTED IN IMPINGEMLWT SURVEYS CONDUCTED FOR ONE 24-HOUR PERIOD PER WEEK,1989 DVPS Number Collected Operating Non-Operating Date Intake Days Intake Bays Month Day Alive Dead Alive Dead January 6 0 0 4 0 13 1 0 0 0 20 1 1 0 0 27 3 0 0 0 February 3 3 0 0 0 10 (a) 2 0 0 0 17(a) 24 March 3 0 0 0 0 10 1 0 2 1 17 1 0 0 0 24 0 0 1 0 31 *I I April 7 2 0 0 0 14 0 0 0 0 21 0 0 0 0 26 0 0 1 0 May 5 0 0 0 0 12 0 0 0 0 19((b) b) 26 June 2 0 0 0 0 9 0 0 0 0 16 0 0 0 0 23 0 0 0 0 30 0 0 0 0 July 7 1 2 0 0 14 4 2 0 0 21 1 3 0 1 28 4 6 0 0 96

no DUQUESHE LIGilT COMPANY 1989 ANNUAL DIV1RONMDATAL REPORT TABLE V-G-6 (Continued) Number Collected Operating Non-Operating Date Intake Bays Intake Days Month Day Alive Dead Alive Dead August 4 1 5 0 0 11 0 4 0 0 18 3 1 0 0 25 2 4 0 0 September 1 5 4 0 0 8 0 2 0 1 15 3 0 0 1 22 0 0 0 1 29 0 2 0 1 October 6 0 2 1 0 13 0 0 0 0 20 0 0 0 0 27 1 1 0 0 'Hovember 3(b) 10(b) 17(b) 24 0 1 0 0 December 1(b) 8(c) 15 (c) 22(C) 29 0 0 0 0 Tot 41 39 40 9 6 I"I Impingement could not be conducted due to high water conditions. (b) Impingement could not be conducted due to diving operations in screenhouse. I'I Impingement could not be conducted due to maintenance. r F l 1 97 l

DUQUESNE LIGilT COMPANY 1989 AllNUAL ENVIlO!DiENTAL REPORT TABLE V-G-7

SUMMARY

OF Corbicuh COLLECTED DURING IMPINGEMENT SURVEYS F')R ONE 24-HOUR PERIOD PER WEEK,1989 BVPS Number Collected Operating Non-Operating _ Date Intake Days Intake Days Month Day give _ Dead Alive Dead January 6 0 1 0 0 13 1 1 0 0 20 4 3 0 0 27 1 5 0 1 t February 3 0 5 0 0 10(a) 0 3 0 0 17 24(a) 31 March 3 0 7 0 0 10 0 3 2 0 f 17 0 0 0 0 24 0 2 0 0 I 31 "I April 7 0 0 0 0 14 0 1 0 2 21 0 0 0 0 28 0 1 0 0 May 5 0 0 0 0 12 3 0 0 0 19(b) 26(b) June 2 0 1 0 ? 9 0 3 0 0-16 1 10 0 0 23 0 0 0 0 30 1 0 0 0 July 7-2 1 0 0 14 -4 3 0 0 21 6 3 0 3 28 5 0 0 0 L 98 -~

DUQ'J.SilE LIGitT COMPA!4Y 1989 AllN 11 L 12WiltOtMENTAL REPORT l TABLE V-G-7 (Continued) Humber Collected Operating Hon-Operating Date intake Days Intake Days _ Month Dy Alive Dead Alive Dead August 4 5 12 0 0 11 12 9 0 0 18 50 40 0 0 25 114 29 0 0 September 1 2,380 218 0 0 8 3,351 48 173 4 15 4,612 135 729 24 22 0 0 62 4 29 933 213 122 70 October 6 868 345 544 36 13 227 41 47 17 20 114 26 63 15 27 232 134 168 124 November 3(b) 10(b) 17(b) 24 28 31 0 0 1((b) December c) 8 15 (c) 22(c) 29 32 31 0 1 TOTAL 12,986 1,373 1,910 300 (a) Impingement could not be conducted due to high water conditions. (b) Impingement could not be conducted due to diving operations in screenhouse. (c) Impingement could not be conducted due to maintenance. 4 9 l 99

~. - - -. .... - ~. ? DUQUESHE LIG!!T COMPA11Y 1989 AllNUAL DIVIltOllMEllTAL REPORT TABLE V-G-B SUM 4ARY OF MOLLUSKS (OTHER TilAN Corbicula) AND DRAGONFLIES COLLECTED IN IMPINGEMDiT SURVEYS CONDUCTED TOh ONE 24-IIOUR PERIOD PER WEEK,1989 BVPS Date Number of Organisms in all Bays Month Dy Mollusks Dragonflies January 6 0 0 13 1 0 20 1 0 27 1 0 February 3 1 0 10 (a) 3 0 17 24 (a) March 3 1 0 10 0 0 17 0 0 24 (a) 3 1 31 April 7 0 0 J4 1 0 21 0 0 28 0 2 May 5 1 2 12(b) 3 0 19 26(DI June 2 0 0 9 0 0 16 3 3 23 0 0 30 0 1 July 7 0 1 14 1 0 21 0 1 20 0 1 l 100

ese DUQUCSi1E L1GitT CWiPAllY 1989 A11110AL 1siVIltoletDITAL ltEront TABLE V-G-8 (Continued) Date liumber of Organisms En all Days Month DR Mollusks Dragonflies Augus' 4 0 0 11 1 0 18 0 1 25 3 7 September 1 6 6 8 13 5 15 12 2 22 1 0 29 5 3 October 6 4 0 13 0 2 20 6 3 27 63 5 llovember 3(b) 10(b) 17(bs 24 1 1 December 1(b) 8(I c) 15 (CI 22 c) 29 2 0 Total 137 47 ("I impingement could not be conducted due to high water conditions. (b) Impingement could not be conducted due to diving operations in screenhouse. (c) Impingement could not be conducted due to maintenance. 5 101

DUQUESNE LIGitT COMPA!W 1989 ANNUAL E!WIRONMENTAL REPolti y mparison of operating and Won-Operating Intake Bay Collections Of the 550 fish collected during the 1989 leapingement stuales, 524 (95.3%) s ere collected f rom operating intake bays and 26 (4.7%) from non-operating Antake bays (Trolo V-G-2). Ilowever, due to differences between the number of operating (120) and non-operating (28) screens washed in 1989, the 1:spingement data were computed with catch expressed as fish per 2 1,000 m of screen surf ace area washed. These results showed 24.5 and 5.2 fish for operating and non-operating screens, respectively. As in previous years, the numbers of fish collected in non-operating bays indi-cate that fish entrapment, rather than impingement, accounts for some of the catch. Entrapment occurred when fish were lifted out of the water on the frame plates as the traveling screen rotated. Alternatively, lapingement occurred when fish were forced against the screen due to velocities created by the circulating wter pumps. Of the 94 crayfish collected in the 1989 lapingement studies, 79 (84.0%) were collected from operating bays and 15 (16. 0 % ) were collected from , non-operating bays (Table V-G-6). Adjusting these data for screen sur-2 face area washed (crayfish per 1,000 m ) the results show 3.7 and 3.0 crayfish for operating and non-operating screena, respectively. corbicula collected in the 1989 studies included 14,359 (86.6%) in the operating bays and 2,218 (13.4%) in the non-operating bays (Table V-G-7). Again, adjusting these data for the screen surface area washed 2 (corbicula per 1,000 m ) the results show 671.0 and 444.2 Corbicula for operating and non-operating screens, respectively. Summary and Ccnclusions The results of the 1989 lapingement surveys indicate that withdrawal of river water at the BVPS intake for cooling purpcses has very little or no effect on the fish populations. Pive hundred and fif ty (550) fishes were collected, which was the fourth highest total collected since initial operation of BVPS in 1976. Gizzard shad were the most numerous fish, comprising 79.5% of the total annual catch. The total weight of all 102

DUQUESNE LIGirr COMIW1Y 1989 ANNUAL DiVIR0tiMENTAL REPORT l fishes collected in 1989 was 4.05 kg (8.9 lbs). Of t.he 550 fishes col-1ected, 41 (7.5%) were alive and returned via the discharge pipe to the Ohio River. PIANKTOt1 D1TRAINMDIT 1. Ichthyoplankton objectives The ichthyoplankton entrainment studies are designed to determine the species composition, relative abundance, and distribution of ichthyo-plankton found in proximity to the BVPS intake structure. Methods Previous studios have demonstrated that species composition and relative abundance of ichthyoplankton samples collected in front of the intake structure were very siellar to those ichthyoplankton entrainment samples taken at DVPS (DLC 1976, 1977, 1978, and 1979). Based on these results, a sodified sampling program was utilized from 1980 through the current sampling season which sampled the Ohio River along a transect adjacent to the BVPS intake structure (Figure V-F-1). Samples were collected monthly, f rom April through August, during daylight hours along a five station transect. Night collections were made in May and July. Surface town were made at Stations 1, 3, and 5 and bottom tows were taken at Station 2 and 4 utilizing a 505 micron mesh plankton net with a 0.5 m diameter mouth. Sample volumes were measured by a General Oceanics Model 2030 digital flowmeter mounted centrically in the nouth of the net. Samples were preserved upon collection in 5% buf fered formalin containing rose bengal dye. In the laboratory, eggs, larvae, juveniles, and adults w, e sorted from the samples, identified to the lowest possible taxon and stage of devel-3 opment, and enumeratc:d. Densities of ichthyoplankton (number /100m ) were l calculated using appropriate flowmeter data. l 103 l

DUQUESNE LIGirr COMPANY 1999 ANNUAL ENVIRONHENTAL REPORT Results A total of 3,016 eggs,.3,139 larvae, and two adults representing eleven 3 taxa and seven familes were collected from 4039.2 m of water filtered during sampilng along the river entrainment transects (Table V-II-1). Gizzard shad, freshwater drum, and shiners were the most common ta xa, representing 27.34, 56.6%, and 8.$4 of the total catch. G12 ard shad compel..e d 53.5% of the larvae. Frerhwater drum comprised 18.4% of the larvae. Eggs (3,016) made up 49% of the total lehthyoplankton catch. Seasonal Distribution _ No eggs were collected during the first survey (April 13). On the day and night surveys (May 23, 24) (Table V-H-1), two and three eggs were collected, respectively. The day collection of June 19 rsaulted in a 3 total density of 15 56/100 m. The July 12 (day) collection yleided a 3 total density of 229.73/100 m of which Iseshwater drum, shiner sp., and gizzard shad comprised the majority of the catch. The August 15 (day) 3 collection a,howed a decreased total density of 13.70 /100m (Table V-Il-1). 3 Greatest density. (768.01/100 m ) was obtained on the night of July 13.. This was due to a large catch of f reshwater drum eggs (Ta.ile V-H-1). Spatial Distribution Larvae were dominant at all sta tions! however, highest densities were collected at Statione 2, 4, and 5 during night tows. Most of the larvae collected were gizzard shad and freshwater drum. Stations 1, 2, 3, 4, and 5 yielded 701, 561, 310, 434, and 1,133 larvae, respectively, i 104

t i TAEt T-9-1 NUfeet AfE3 Dete!TY {). Fler 30GE, LAPWhE, JUTWE11ES, AEED ADULTS i t# umber /100 m COM WIS A 3.5 m PLassitTop er AT BE BF5tAlm RITOR M IN ME MIO RITtst 30AR WTPs, *.909 i Total Collected and ~ Date Station 1 Station 2 ,Stetten 3 Statloa 4 Statte 5 Tamra De s tty i j g riet g paet M useht g niet g uset apr11 13 + 1 1 i Tel. water filtered (m") 125.0 152.5 130. 7 135.7 133.7 695.6 ( aumber eggs collected d 0 0 0 0 0 j Mueer larvae collected 0 0 0 0 0 0 shamber jueentles collected 0 0 'O O O O w l j maaper adulta collected 2 0 0 0 0 2 } Desity Immber collected) to i Adults i meropis otherinoides 1.59 (2) 0 0 0 0 0.29 (2) b 4 Total station Density 1.59 (2) 0 0 0 0 0.29 (2) j ' immber collected) 1

>' t's t* tts may 23/24 tu l

Vol. water filtered ts ) 109.5 94.2 134.3 112.E 126.4 125.2 126.6 129.7 95.7 111.9 1.1s0.1 $C 3 { wmber eggs collected 1 1 1 0 0 0 0 2 0 0 5 gc l m Dumber Larese collected 2-4 0 3 1 1 3 2 0 12 3s $q }' Munner jareniles collected 0 0 0 0 0 0 0 0 0 0 0 I Musber a&alts collected. 0 0 0 0 0 0 0 0 0 0 0 UO I Density tausber collected) I E998 t- > Morone opp. 0 0 0 0 0 0 0 0.77 (1) 0 0 0.03 (1)

i:

Dnidentified 0.91 (1) 1. 04 (1) 0.74 (1) 0 0 0 0 0.77 (1) 0 0 0.34 g4) gK La re.e Cyprinidae (EL) 0 0 0 0.09 (1) 0 0 0 0 0 0 0.03 (1) Q Catontamidae tc.) 0

1. 04 (1) 0 0.09(1) 0.79 G) 0 0

0.77 (1) 0

2. 60 (3) 0.60 (7)

.g i g morone enrysops (YL) 0 0 0 0 0 0 1.50 (2) 0.77 (1) 0 0 0.26 (3) Pommis opp. (E) 1.03 (2) 2.00 (2) 0 0 0 0 0 0 0 0 0.34 qq } Etheostamm app. (EL) 0 0 0 0 0 3 0 0 3.13 (3) 3.57 (4) 0.50 (7) stasestedmon opp. (YL) 0

1. 04 (1) 0 0.09 (1) 0 0.00 (1) 0.79 (1) 0 5.22 (5) 4.47 (5) 1.2D (14)

} Total station Density 2.7* (3) 5.20 (5) 0.74 (1) 2.54 (3) 0.79 G) 0.00 (1) 2.37 (3) 3.00 (4) 0.36 (s) 10.72 (12) 3.51 (41) { t=mumber collected) i I I t i a

1 ( i t t '7 mat.E v-s-1 (Centiawed) htal Collected and i Date Statio r 1 Station 2 Statios 3 Station 4 station 5 _Teme tw eity M Baght M pasht g pight M Wight M Witbt -June 19 3 vol.. water flitered (m ) 59.3 54.5 .77.6 78.1 e4.0 35 3.5 mumber egge collected 2 1 1 0 1 5 pueer larvee eclieeted. 4' 3 5 14 24 50 i teumber joveniles collected 0 0-0 0 0 0 number a.3ults collected 0 0 0 0 0 0 i Density (nu e er collected) [ Eggs [ Dnidentified 3.37(2) 1.83(1) 1.29(1) 0 1.19(1) 1.41 (5) i Larvae e 9 Derasome cepedianar (EL) 0 0 0 5.12 (4) 4.76 (43 2.26 (8 ) ce Cyprinadee (2:) 0 0 0 1.28 (1) 0 0.28 (1) .l CYprinos corpio (fL) 0 0 6 2.56 (2) O c.57 (2) gg [ Cyprinus carpio (22.)

6. 75 (4) 5.50 (3) 6.44 (5) 8.96 (7) 7.14 (6) 7.07 (25)

Eo Macropteros dolonjeci (EL) 0 0 0 0 2.38 (2) 0.57 (2) cc L Etheosteen opp. (EL) 0 0 0 0 14.2e (12) 3.39 (12) [$ [ Total station Density 10.12 (6) 7.34 (4) 7.73 (6) 17.93 (14) 29.76 (25) 15.56 (55,

E

{ (numer collected) QM j < t* July 12/13

• • y l

y h= 'o 3 vol. water filtered (m 3 ' 122.0 114.5' 133.6 118.5 123.9 120.3 136.9 120.1 127.4 112.4 1.22,.6 2: *5 i

h. n number egos collected 12 107 22 243 24 199 6

1.516 8

• 19 2.956 i

museer larvae collected 324 362 110 441 16 8 135 15 6 251 649 426 3,022 -Q $ CJ musber juveniles collected 0 0 0 0 0 0 0 0 0 0 0 + wumber adults collected 0 0 0 0 0 0 0 0 0 6 0 t* g Density (number collected) pQ [ Eggs 9.84. 89.08 16.47 1 35. 86 193.70 161.26 4.38 1.239.78 6.28 726.87 232.51 3 M t Aplodinotes grunniens (EE) (12) (102) (22) (16 1) (24) (1941 (6) (1513) (8) 1817) (2859)

o E

f

• 3 Unidentified 0

4.37(5) O 69.20(82) 0 4.16 (5) 0 2.50(3) 0 1.7s(2) 7.s9(97) Larvoe Dorosoem cepedianos (TL) 4.} 0 (5 ) 11.35(13) 7.49(10) 24.47(29) 3.23(4) 18.29(22) 6.57(9) 36.64(44) 2.35 (3) 3.56(4) 11.63(143) Dorosons cepeatanum (EL) 173.77. 192.14 28.44 124.89 99.27 8' 72 48.94 79.93 299.84 165.48 124.19 (212) '(220) (38) (140) (123) r$5) (67) (96) (382) (186) (1.5 27) i Cyprinidee (YL) 0 0 0 4.22 (5) 0 1.66 (2) 0 4.16 (5) 0 5.34 D) 1.46 (le) Cyprinidae (EL) - 0 0 5.99(8) 0 4.84(6) 0 0 0 0 0 1.14(14) Cyprinus carpio (EL) 0 25.23 (29) 12.72 (IT) 1.69(2) 1.61(2) 4.99 (6) 7.30 (10) 2.50 (3) 0.78(1) 64 c s72) 11.55(142) potropas otherinoides (EL) 0 4.37 0 ) 0 0 1.61(2) 0 0 0 1.57(2) 4.45(3) 1.14(14) metrapis opp.

68. 85 (84) 31. 44 (36) 1.50(2) 5.91(7) 16.14 (2Cl 4.9946) 6.57(9) 5.00tt) 169.54 (216396.98(109) 40.26 (495) pimepesies app. (EL) 13.93(11)

7. 86 (9) 0 0

3.2314) 0.83(1) 0 o 24.33(11) 8.01(9) 5.77(71) storen, erreops (TL) 2. 46 (3) 0 0 0 0 0 0 0 0 0 0.24(3) i posemis opp. (EL) 0 0 0 0 0 0.83(1) 0 0 0 } c.08 (1) Etwostman opp. (EL) 1.64(2) 2.62(3) 0 0 0 0 0 0 1.57(2) 0 0.57(7) Aplodinotse grWRniens (YL) 0 31.31(37) 19.46(26)191.56(227) 0.81(1) 29.09(35) 40.18 05) 77.44(93) 8.63(11' 16.90(19) 40.99 004) Aplodinotas Lrunnteni (EL), 'J 6.11(7) 5.24 (?) 16.03 (19) 2.42(3) 4.16 t5) 2.92(4) 2.50(3) 0 14.23(16) 5.20(64) i f I . ~

TAsiz v-a-1 (Ctritirased) html Collected and Dete Station 1 Staticm 2 Station 3 Station 4 Station 5 Teve D-sity M

1. tet M

pacht M Might Dev sight g Might July 12/13 (cont'F) tm16entifiable (*L) 0.82(1) 2.62(3) 1.50(2) 3.38(4) 2.42(3) 1.66 (2) 1.46(2) 0.83(1) 0.78(1) 0 1.55(19) html statica Density 275.41 Gws.61 98.80 577.22 154.96 277.64 118.33 1.471.27 5 15.70 1.107.85 486.17 (number collected) (336) (469) (132) (684) (192) (334) (162) (1.767) (657) (1.245) (5.978) August 15 W1. water filtered in ) 114.6 115.5 120.2 123.4 117.7 591.4 3 museer eggs collected 9 11 6 8 16 50 number larvae collected 5 4 0 8 14 31 mzuber joveniles collected 0 9 0 0 0 0 Number adults collected 0 0 0 0 0 0 e Daneity (rmanber collected) g gy Eggs Aplodinotes grunniens 7.85(9) 8.66(10) 4.99(6) 6.48(8) 13.59(16) 8.29(49) C Z Unidentif aed 0

f. 87 (1) 0 0

0 0.17(1) CCpn 1 re.e Dorosons,eepa5 inwe (EL) 0 0.87 (1) 0 0 0 0.17(1) petropas atrierimioes (E:,) 4.36 (5 ) 0 0 0 8.50(10) 2.54 (15 ) gE N'. b Pimephales spp. (EL) 0 0 0 0 3.40(4) 0.68(4) a Aplodinotus grunniens (TL) 0 1.73(2) 0 1.52(2) 0 0.68(4) y Aplod tnetos grunniens (EL) 0 0.87(1) 0 4.86(6) 1.18[7s y9 -4 Total Station Denstry 12.22(14) 12.99!15) 4.91(6) 12.97(16) 25.49 (33) 13.70(81) ~y b (nu1mber cc11ected) an 5 Eh l Tear?y wtal W1. water filtered ta ) 531.4 210.7 600.4 231.1 586.8 245.5 600.7 249.8 558.5 224.3 4.039.2 3 Number eggs collected 24 108 35 243 31 199 14 1.5 18 25 819 3.016 mumber larese.aollected 333 366 117 444 174 1 36 181 253 695 438 3.139 g Museer adulti collected 2 0 0 0 0 0 0 0 0 0 2 i Density truseer collected) Eggs l Morone opp. 0 0 0 0 0 0 0 0.40(1) 0 0 0.02(1) Aplodinotus grunnies 3.95 48.41 5.33 69.67 5.11 79.02 2.33 605.68 4.30 364.24 71.99 (21) (102) (32) (161) (30) (194) (14) (1.513) (2 4 ) (817) (2. 908) Unidentif M 0.56(3) 2.85 (6) 0.50(3) 25.48(82) 0.17 (1) 2.04 (5 ) 0 1.60(41 0.18 (1) 0.89(2)

2. 65 (107)

Larese Inrosrms cepeetanus (TL) 0.94(5) 4.17(13) 1.67(10) 12.55 (29) 0.68(4) 8.96(22) 1.50(9) 17.61(44) 0.54 (3) 1.78(4) 3.54(143) Dorosene c pediame (tL} 39.89 104.41 6.50 64.04 20.96 22.40 11.82 38.43 69.11 82.92 38.03 (212) (225) (39) (148) (123) (55) (71) (96) (386) (186) (1.5 36) f Cyprinidae (TL) 0 0 0 2.16 f.5) 0 0.81(2) 0 2.03 t3 ) 0 2.67(6) 0.45(18) { cyprinidae (EL) 0 0 1.33(8) 0.43(1) 1.02?6) 0 0.17(1) 0 0 0 0.40(16) I Cyprinue corpio (TL3 0 0 0 0 0 0 0.33(2) 0 0 0 0.05 (2) Cyprinus carpio (EL) 0.75(4) 13.76(29) 3.33(20) 0.87(2) 1.19 0) 2.44(6) 2.83(17) 1.20(3) 1.25 (7) 32.10 02) 4.13(167) potrevis atherancides (EL) 0.94(5) 2.37(5) 0 0 0.34(2) O O 2.15 (12) 2.23[5) 0.72 (29) l =

= i TABLE D-9-1 ) (Continued) l Mtal Collected and Date Statim 1 Station 2 Station 3 Station 4 Stathon 5 Tema D. netty M Nacht M sight M le!qht M Might g Night Yearly Wral (cet 'd) Notreois e;p. (EL) 15.81(84) 17.01(36) 0.3)(2) 3.03(7) 3.41(20) 2.44(6) 1.50(9) 2.40(6)

38. 68 (216 ) 48.f 0 (109 ) 12.25(495)

Famepnaies S;p. (C) 3.20 (17) 4.27 (9) 0 0 0.68(4) 0.41 (1) 0 0

6. 27( 5 ) 4.01(9)

1. 06 (75 )

Catastomidae (EL) 0 0.47(1) 0 0.43(1) 0.17(1) 0 0 0.40(1) 0 1-34(3) 0.17(7) mrone chryses (YL) 0.56(3) 0 0 0 0 0 0.33(2) 0.40(1) 0 0 1.15 (6 ) Micropteros colosjeol (EL) 0 0 0 0 0 0 0 0 0.36 (2) 0 0.0$ (2) Paeouts esp. (G) 0.38(2)

0. 95 (2) 0 0

0 0.41 (1) 0 0 0 0 0.12(5) w* Etmetene opp. (EL) 0.38(2) 1.42(3) 0 0 0 0 0 0 3.04 (17) 1.78(4) 0.64 (26) Ettrostedten app. (YL) 0 0.47(1) 0 0.43(1) 0 0.41 (1) 0.17 (1) 0 0.90(5) 2.23(3) 0. 35 (14) e i Aplodinotes grunniens (YL) 0 17.56(37) 4.66(28) 98.23(227) 0.17(1) 14.26(35) 9.49(57) 37.23(93) 1.97(11) 8.47(19) 12.58(508) I Ap2odinotus grunnae vs (c) 0 3.32 (7) 1.33(8) 8.22 (19) 1.62(3) 2.04 (5) 1.46(10) 1.20(3) 0

7. 13 (16 )

1. 76 (71)

$h =O l tmadentif sable 0.19(1) 1.42(3) 0.33(2) 1.73(4) 0.51(3) 0.01(2) 0.33(2) 0.40(1) 0.18(1) O C 47 g19) h$ l Adults leotrepis ortherinoides 0.38(2) 0 0 0 0 0 0 0 0 0 0.05 (*, C* $ ~ UM Mtal Station Density 67.93 224. 96 25.32 297. 27 34.94 136.46 32.46 708.97 128.92 560.41 152.43 (number collacted) (36 1) (474) (15 2) (687) (205) (3 35 ) (195) (1.771) (7 22) (1.25 7) (6.15 7) NC 39 za =

r Dm*cpmentsi Stapes Cn Qk TL ~ Ratched specimens rith yolk and/or oil 91ceules present.

EL - Specimens with F., yolk and/cr oil globules and with no developmert of von rays 6 4/or opiny elements. [y LL ~ Specimens with developed f an rays and/or spiny elements and evidence e fin fold. = MK

• L - Spacimene with undafinable Astral st. age due to damaze or deterioratic

.7J ~ Specimen,.rith conplete fin and pigment 6-velopment, i.e., ismaature a. h O MH

em DUQUESi4E LIGilT COMPAliY 1989 ANiiUAL DiVIRotiMalTAL REPORT ,Surunary and Conclusions The similarity of species compositicn and relative abundance of ichthyo-plankton taken in 1989 along the river transect to those of 1979-1988, combined with the close c.strelation between river sampling in front of the intake and actual entrainment sampling established in previous years (DLC 1976, 1977, 1978 and 1979) suggest little change in ichthyoplankton entrainment by DVPS in 1989. 2. Phytoplankton objectives The phytoplankton entrainment study was designed to determine the compa-sition and abundance of phytoplankton entrained in the intake water sys-tem. Methods After April 1, 1980, plankton sampling was reduced to one entrainment sample collected monthly. A one gallon sample was collected f rom below the skimmer wall from one operating intake bay. In the labor a tor y,- phytoplankton analyses were performed in accordance with procedures described in Section C, PilYTOPIANKTQ1. Total densities (cells /ml) were calculated for all taxa. Ilowever, only densities of the 15 aost abundant taxa each month are presented in Section C of this report. Comparison of Entrainment and River Samplen Plankton samples were not collected at any river stations af ter April 1, 1980 due to a reduction of the Aquatic Monitoring Program, therefore, comparison of entrainment and river samples was not possible for the 1989 phytoplankton program. Results of phytoplankton analyses for the entrainment sample collected r.cnthly are presented in Section C, Pl!YTO-PLANKTG1. 109 l

---._._~ DUQUESHE LICl!T COMPANY 1989 ANNUAL DWIR0liMD1TAL REPORT During the years 1976 throught 1979, phytoplankton densities of entrain-ment samples were usually slightly lower than those of mean total densi-ties observed from river sampics (DLC 1900). Ilowever, the species com-position of phytoplankton in the river and in the entrainment samples were similar (DLC 1976, 1977, 1979, and 1980). Studies from previous years indicate mean Shannon-Weiner indices, evenness and richness values of entrainment samples were very simliar to the river samples (DLC 1979, and 1980). Summary and conclusions Past results of monthly sampling of phytoplankton in the Ohio River near DVPS and within the intake structure showed little difference in densi-ties (cells /ml) and species composition. During periods of minimum low river flow, approximately $4 of the river would be withdr awn Anto the condenser cooling system. Dased on the similar denalties of phytoplank-ton in the river and the DVPS intake structure, and the small amount of water withdrawn from the river, the loss of phytoplankton was negligible, even under worst case low flow conditions. 3. Zooplankton Objectives The zooplankton entrainment studies were d. signed to determine the com-position and abundance of zo >planen ent' ined in tb'. ant.au water sys-tem. Methods Plankton entraineent samples were collected and zooplankton were counted. For the zooplankton analyses, a well-mixed sample was taken and processed using the same procedures described in Section D, 200PLANKTOfi. After April 1, 1980, plankton armpling was reduced to one entrainment sample collected monthly. A one gallon sample was collected from below the skimmer wall from one operating intake bay. 110

DUQUESNE LIGHT COMPANY 1989 ANNtJAL ENVIRONMENTAL PEPORT j Total densitier (number / liter) were calculated for all taxa, however, only taxa which comprised greater than 2% of the total are presented in Section D, 2OOPLANKTON. comparison of Entralnment and River Samples Plankton samples were not callected at any river stations af ter April 1, 1980 due to a reduction of the Aquatic Monitoring Program, therefore, comparison of entrainment and river sampics was not possible for the 1989 zooplankton program. Results of zooplankton analyses for the entrainment satale collected monthly are presented in Sect.lon D, ZOOPLANKTON. During past years, composition of zooplankton was similar in entrainment and river samples (DLC 199',). Protozoans and rotif ers were predominant, whereas crustaceans were sparse. Densities of the four most abundant taxa for each month (DLC, 1:,7 6, 1977, 1979, and 1980) indicate the same taxa were present in both river and intake samples. In addition, they were present in similar quantitles. Shannon-Weiner indices, evenness, and richness values for river and entrainment samples were also similar, further demonstrating similarity between entrained and river zooplankton. Summary and Conclusions Past results of monthly sampling of zooplankton in the Ohio River near BVPS and within the intake structure showed little dif ference in densi-ties (number / liter) and species composition. During periods of minimum, low river flow, approximately 5% of the river would be withdrawn into the condenser cooling system. Based on the similar densities of zooplankton in the river and the BVPS intake structure, and the small amount of water withdrawn from the river, the loss of zooplankton was negligible, even under worst case low flow conditions. 4 111

DUQUEfCNE LIGl!T COMPANY 1989 ANNUAL ENV2RONMENTAL F4POPT I. Corbicula MONITORING PROGRAM O Introduction The introduced Asiatic clam, Corbicul1 fluminea (Figure V-I-1), was first detected in the United States lo 1938 in the Columbia River near Knappton, Washington (Burch 1944). It has since spread throughout the sountry, inhabiting any suitable freshwater habitat. Information from prior aquatic serveys has demonstrated the presence of Corbicula in the Ohio River in. the vicinity of the BVPS, and the plant is listed in NUREG/CR-4233 (Counts 1985). One adult clam is capable of producing many thousands of larvae called veligers. Tnese veligers are very small (approximately 0.2 mm) and will pass easily through the water passages of a power plant. Once the veli- - ger settles to the substrate, growth of the clam occurs rapidly. If clams develop within a power plant's water passages, they impair the flow of water through the plant. Reduction of flow may be so severe that a - plant shutdown is necessary, a* occurred in 1980 at Arkansas Nuclear One Power Plant. The clams tre of particular concern when they develop undetected in emergency systen c ht - ^he flow of water is not constant fHRC, IE Bulletin 81-03). The Corbicula Moultoting Program includes the Ohio River and the circu-lating cc311ng water system of the BVPS (intake structure and cooling towers). This report describes this Monitoring Program and the results obtained dur1@ lield :.nd ;.lant surveys conducted through 1989. 1. Monitoring O_bjectivus The two objectives of the Monitoring Program were to evaluate the pres-ence of Corbicula at the BVPS and to assess the population of Corbicula in the Ohio River in order to evaluate the potential for infestation of the BVPS. 112

-. -. - ---. _.. ~... -. -.. ~.. - DUQUESNE LIGitT COMPANY ANNUAL EtWIRONMENTAL REPORT 1 f'f f _a dk._ l 1 I cm l I I q l 2 3 l Cm. ? 4 l Cody 1995, Aquatic Systems Corporation Photographs 1 and 3 show key characterirtic (serrated hinges) for genus level identifici. tion I FIGURE V-I-1 PHOTOGRAPHS OF Ccrbicula COLLECTED AT l BVPS i c.<- ,7

DUQUESNE LICirt COMPANY 1989 ANNUAL DWIRONMENTAL REPORT Methods (Unit 1 Cooling Tower) Collections were made (September 7) in the upper and lower recervoirs of Unit I cooling tower during a scheduled outage. Samples were collected using a (6" x 6") petite ponar dredge at the east side in the upper reservoir. The lower reservoir was sampled at seventeen (17) stations within the cooling tower (FJ gure V-I-2). (Unit 2 Cooling Tower) Collections were made (March 21) in the reservoir of Unit 2 cooling tower during a scheduled outage. Ten samples were collected using a (6" x 6") petite ponar dredge within the area that contained sediment. (Figure V-I-3). The substrate of each sample was characterized at the time of collec-tion. The samples were then returned to the laboratory and sorted for Corbicula within 72 hours of collection. This procedure increased over-all sorting efficiency because formalin, normally used to preserve the samples for long periods of time, was not needed and live Corbicula coult be seen moving in the sor ting trays. Counts were made of live and dead Corbicula for each dredge sample. These counts were converted to densi-2 ties (clams /m ) for each collection based on the surf ace area sampled by the dredge. (Intake). Plant operations personnel have the intake surveyed semi-annually by divers for allt buildup, and if necessary, the intake bays are cleaned. Cleaning of all four bayr occurred in May and November 19S9, by divers using a Flygt 20 hp submersible pump. This pump has a capacity of 500 ^ gpm ' (1,750 rpm) and uses a five inch propeller to push water and debris through a flexible hose (Jenkins and Logar 1985). Water and debris were sluiced through the drainage system of the intake structure, where some of the larger clam shells remained after the cleaning operations. 114

am DUQUESNC LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT ('IWO DIMENSIONAL: CROSS SECTIONAL IlORIZONAL VIEW) N q 7 W ) l_ 12-l ll 8 l i e e i 13 STAIRWAY g/ l 9 e e l [ I 17 ~ 98 10 e e e l4 83 STAIRWAY { N a e 9 4 L S l' / 7' e 5 6 WATER OUTLET i o EQUIPMENT / t ACCESS RAMP 50 FEET l l e SAMPLE LOCATION WITHIN THE LOWER WATER RESERV0lR l l l FIGURE V-I-2 -Corbicula MONITORING PROGRAN SAMPLING STATIONS OF THE LOWER RESERVOIR OF UNIT 1 COOLING TOWER BVPS l 115

DUQUESilE LIGilT COMPA!1Y 1989 Al?tlUAL 12iVIROtiMEllTAL REPORT (TVO DIMENSIONAL: CROSS SECTIONAL llORIZONAL VIEW) WATER OUTLET b 3-STAIRWAY .JA.. i... 1 y

y

!!.1 3 O WATER COLUMNS .....:; i [.%!i* - PONAR GRAB SAMPLE O 2o.1:/.... ' -~ f!i i O . f.?d!i. AREA OF SEDIMENT , Q:.

hhi 3:::

hII Mii:jjpi i.gyi: i i...... STAIRWAY 50 FEET FIGURE V-I-3 Corbicula MONITORING PROGRAM SAMPLING STATIONS OF THE LOWER RESERVOIR OF UNIT 2 COOLING 2DWER BVPS 116

DUQUESNE LIGi!T COMPANY 1980 ANNUAL ENVIRONMENTAL REPORT (River) Samples were collected using either a regular Ponar (9" x 9") or a petite Ponar (6" x 6") dredge along transects across the river. Ten transects were established along the Ohio River: four upstream, five downstream and one at the plant intake. A transect was also established on Baccoon Creek (Figure V-I-4). Two transects below the BVPS were divided where samples were taken on either side of Phillis and Georgetown Islands. Each transect was based on suitable substrate (e.g., sand and/or gravel) or heated discharge (HD). Each station was identified by river navigation mile (Figure V-I-4). In May and September, samples were collected which included a single left shore, right shore, and mid-channel station. The substrate of each sample was characterized at the time of collec-tion. The samples were then returned to the laboratory and sorted for Corbicula. Counts were made of live and dead Corbicula for each dredge 2 sample. Live clam counts were converted to densities (clams /m ) for each collection based on the surface area sampled by the dredge. Results (Unit 1 Cooling Tower) Results of the September */ Corbicula survey of the Unit 1 cooling tower are presented in Table V-I-1. Densities were calculated only for live Corbicula, as densities for empty shells do not translate into potential colonizers, and such figures could be distorted by the redistribution of dead clams by currents. No live Corbicula were collected in the upper reservoir; however, the presence of shells indicates that they were transported within the circulating water system. Based on the 17 Ponar grab samples taken from the lower reservoir, the estimated number of Corbicula inhabiting this crea was 300 million, of which 98% were alive (Figure V-I-5). Total length ranged from 1. 0 mm to 29.0 mm for clams collected from the lower reservoir. I17

o BEAVER RIVER N ROuto A m / 28.2 3EAVER \\ 'N\\ 30.V 4 h cot &AY \\ \\ IA " 33.0 37.5 OHIOVIEW ( 0.2 \\ 37.0 RACCGON \\ g bO MIDLAND ,\\ g r2 P MOffTGOMERY / D L9CKS & DAM j BADEN g5 q f $p 4.v GEORGE'IWN y b 34.5 b= BEAVER VALLEY AL*lOUIPPA kd H* IWER j Q / ^I 35.7 AMBMDGE p 34.8 C t 2 4 Q 35.0 SOALE MILES 3 N \\ sotmt LEGEND ~ HEIGITTSI k---4 SAMPLE STATION PiVER MILE POINT I DASHIELIG IOCK & DAM FIGURE V-I-4 Corbicula MONI'IORING PROGRAM SAMPLING STATIONS OHIO RIVER SYSTEM BVPS

== DUQUESNE LIGilT COMPANY 1989 AllNUAL DIVIRONMENTAL REPORT TABLE V-I-l + Corbicula COLLECTED IN UNIT 1 COOLING TOWER SEPTEMBER 7, 1989 DVPS Clams Collected Station Density Sample Location Substrate Alive Dead LiveClams/mi Upper Reservoir Qualitative Sample sil 0 195 0 (East) Lower Reservoir 1 all 235 9 10,129 2 sil 214 2 9,223 3 sil 188 14 8,103 4 sil 2,695 25 116,155 5 sil 864 8 37,238 6 sil 216 2 9,310 7 sil 622 26 26,808 8 all 92 2 3,965 9 sil 109 2 4,698 10 sil 2,072 37 89,303 11 sil 265 3 11,422 12 sil 171 12 7,370 13 sil 180 4 7,758 14 sil 104 7 4,482 15 sil 125 5 5,388 16 sil 96 3 4,138 17 sil 183 3 7,887 l Substrate codess sil - silt l 8 119

o 350 # l 300 2 300 - c ~Q EE 250 - -2 ~ e, o 2 O 200 - 176 .E 8-E '8 R .?- ?G 4 s z 3 150 - ga 1 . ;$ C t ~g O g e-3 1 100 -- 2 70 g4 -l gn ? a@ s >m O 50 - 18.5 "E "~O 0 ci h ^ .',' L7 8 I iii./,/ ,:i.i.</,., /, ~,, ,./i.,>> i. v./ ,//., /,., O i i,ii e TAJJASOND .JFMAMJJASOND JFMAMJJASOND JFMAMJJAS 86 87 88 89 l* 'lUNIT 1 UNIT 2 i L FIGURE V-I-5 APPROXIMATE POPULATIONS OF Corbicula.IN UNITS I and 2 COOLING TOWERS DERIVED FROM SURVEYS' CONDUCTED IN 1986 THROUGH 1989 BVPS

== DUQUESNE LIGirr COMPANY b89 ANNUAL DufIRONMENTAL REPORT (Unit 2 Cooling Tower) Results of the March 21 Corbicula survey of the Unit 2 cooling tower are presented in Table V-I-2. Based on the ten Ponar grab samples taken from the lower reservoir, the estimated number of clams inhabiting this area was 10.5 million, of which 42% were alive (Figure V-I-5), (Intake) While performing the innerbay cleaning operation (May and November 1989), the divers observed concentrations of Corbicula in each of the bays close to the intake pumps. The November cleaning operation produced more clams than what was removed in the May cleaning operation. Approximately five 55-gallon drums of clams were removed from Bay B and two 55-gallon drums removed from each of the remaining bays during the November operation. A cut-away diagram of the intake structure is provided in Figure V-I-6. (River) The results of the Corbicula survey in the Ohio River are given in Tables V-I-3 (May) and V-I-4 (September ). Dead clams were not counted in sam-ples of the regular benthic macroinvertebrate monitoring program. Live Corbicula were collected in substrates of silt, sand, and gravel. l l l Substantially fewer Corbicula were collected in May as compared to 2 September's collection. Live density calculations surpassed 100/m 2 eleven times, with the highest density being 862/m at mile 33.0 of the Ohio River in September. Table V-I-5 summarizes Corbicula, frequency in past macroinvertebrate collections for the BVPS (1973 through 1989). Peaks in population den-sity are apparent in the years 1976, 1981, and 1988; no Corbicula were found during 1973, 1979 and 1980. Corbicula densities increased sub-stantially during fall collections. 121

a DUQUESNE LIGilT COMPANY 1909 ANNUAL D4VIRONMENTAL REPORT TABLE V-I-2 Corbicula COLLECTED IN UNIT 2 COOLING TOWER March 21, 1989 BVPS Clams Collected Station Density-Sample Location Substrate , Alive Dead Live Clams /m' Lower Reservoir 1 sil 1 4 43 2 sil 0 0 0 3 all 1 1 43 4 -sil 0 0 0 5 sil 0 1 0 6 sil 0 0 0 7 sil 166 564 7,155 3 sil 44 16 1,096 9 sil 263 376 11,335 10 sil 220 9 9,482 Substrate Codes: sil - silt 9 4 3 122

DUQUESNE LIGHT COMPANY 1989 Atil1UAL DIV.!R0!1ME11TAL REPORT (THREE DIMENSIONAL: CUT AWAY VIEW) ^ ,,sh, TRASH 7 M ARE A CLEAhED BY RACK f DIVING OPERATIOt-m s 4 s s 4 Ac TR AVELING SCR EE i D V ~ t B l h y A A o, " y " Jv l BAY ARE A CLEANED BY DIVING OPERATIONS BAY D (TWO DIMENSIONAL: SIDE VIEW) "y." I [ TRAVELING SCREEN g i / J TRASH RACK ARE A CLE ANED BY

/

DIVING OPERATIONS i / !I p- / ARE A CLE ANED BY DIVING OPER ATIONS FIGURE V-I-6 Corbicula MONITORING PROGRAM SAMPLING STATIONS l-INTAKE STRUCTURE l DVPS 123 I

4 A DUQUESNE LIGi!T COMPANY -1989 ANNUAL DIVIRONMENTAL REPORT TABLE V-I-3 Corbicula COLLECTED IN THE OHIO RIVER MAY 23, 1989 EVPS Class Station Sample River Collected Density 2 Location Mlle Bank Depth (ft.) Substrate Alive Dead Live Claas/m Raccoon Creek 0.3 R 4 s11 0 0 0 M 5 sil/gre/ cob 0 0 0 L 2 sil 0 0 0 Ohio River 28.2 R 1 sil 0 0 0 M 36 gra 0 1 0 L 1 sil 1 1 43 30.0 R 3 cle/sil C 0 0 M 29 gra 0 1 0 L 3 cla/sil 0 0 0 33.0 R 2 sil 0 1 0 M 20 ges/ cob 1 0 43 L 1 s11 1 24 43 III R 2 sil/gra 0 4 0 34.5 M 20 gra/ cob 0 0 0 L 2 mil 0 0 L 2 s!! O 0 34.8-R 2 all 0 0 0 M 22 gra/ cob 0 0 0 L 20 sil 0 10 0 4 (Back Channel) 35.0 R 2 cla/sil/ san 0 1 0 M 30 sil/ san 0 1 0 L "DI 1 s!! 0 0 0 I 35.4(2A) R-3 gra 0 0 0 M 19 san /gra 0 1 0 L 2 cla/ san 0 0 L 2 cle/ san 0 0 (Back Channel) 35.4(28),. 2 mil / san 1 20 M 11 gra/ cob 5 99 L 1 sil 0 0 (Back Q.annel) 35.1 R 2 s11 0 1 0 M 14 gra/ cob 0 1 0 L 3 gra 0 1 0 37.0I3I R(HD) 2 sil/ san 0 2 0 M 22 gra/ cob 3 0 0 L 1 s11 1 20 L 1 s11 0 0 37.5 R 3 sil/ san 1 1 43 M 22 bed 0 0 0 L 2 san / cob 0 0 0 (Back Channel)37.5 R 2 sil 0 0 0 M 16 san /gra/ cot 1 3 43 L 4 san 0 1 0 Substrate Codens Footnotes bed - bedroct (HD) - Heated Discharge cit.- clay (1) - Transact I cob - cobble (2A) - Transect 2A (Ma.9 Channel) det - detritus (23) - Transect 2a (Bac. Channel) gra - gravel (3) - Transect 3 san - sand all - silt l 1 l l I 124 l u

sus DUQUESHE LIGHT COMPANY 1989 ANNUAL ENVIROldiENTAL REPORT TABLE V-I-4 Corbicula COLIECTED IN THE 0410 RIVER SEPTEMBER 14, 1989 BVPS Class Gtation Sample River Collected Density 2 Location Mile Bank Depth ift.) Substrate Alive Dead Live Class /s Raccoon Creek 0.3 R 4 sil 0 1 0 M 6 sil/ san 1 1 43 L 2 sil 0 0 0 Ohio River 28.2 R 2 s il/de t 1 0 43 M 35 san /gra 1 0 43 L 3 sil 0 1 0 4 30.0 R 2 sil/ san 1 3 43 H 38 san /gra 0 1 0 L 6 sil 2 2 86 33.0 R 5 sil/ san 2 3 86 H 21 san /gra 20 3 862 L 4 sil/ san 5 9 216 III R 3 mil / dan 7 9 302 34.5 M 21 gra 0 0 0 0 L 3 sil/ san 0 L 2 sil/ san 1 39 34.8 a 4 sil 0 2 0 M 22 san /gra 2 0 86 L 20 sil/det 9 12 388 (Back Channel) 35.0 R 9 sil 9 9 388 M 25 all/ san /gra 2 3 86 IHDI 3 s!! 0 2 0 L 3 5. 4 (2 A) R 2 cob 1 1 43 M 17 san /gra 5 2 216 158 L 2 cla/ san 8 L 2 c.la/ san 4 79 (Back Channel) 35.4(28) R 2 sil 13 256 M 12 gra/ cob 2 39 L 3 sl1 15 296 (Back Channel) 35.7 R 3 all 2 4 86 M 12 san /gra 0 0 0 L 3 sil/det 2 9 86 I33 R(HD) 3 sil/ san 3 3 129 37.0 H 21 cob 0 2 0 L 1 sil 8 158 L 2 sil 3 59 37.5 R 3 san 2 5 86 M 22 gra 0 0 0 L 4 san 0 2 0 (Back Channel) 37.5 R 5 s!1 1 3 43 M '. 4 san 1 2 43 L 4 sil/ san /gra 2 7 86 Bobstrate Codes: Footnotes: bed - bedrock (HD) - Heated Di= charge cla - clay (1) - Transect 1 cob - cobble (2A) - Transect 2A (Main Channel) det - detritus (28) - Transect 2B (Back Channel) gra - gravel (3) - Transect 3 san - sand j sil - silt i 125

.,,a DUQUESNE LIGHT COMPANY 1989 ANNUAL DWIRONMENTAL REPORT A TABLE V-I-5 2 Corbicula DENSITIES (Clams /m ) SUMMARIZED FROM BENTHIC MACROINVERTEBRATE COLLECTIONS 1973 THROUGH 1989 DVPS TRANSECT 1 2A 28 3 Back Date L ti R L M R Channel L M R 1973 Nov 0 0 0 0 0 0 0 0 0 0 1974 May 0 0 0 0 0 0 0 0 0 0 Jun 0 0 0 0 0 0 0 0 0 Jul 0 0 0 0 0 0 0 0 0 Aug 0 0 0 0 0 0 0 0 0 Sep. 0 0 7 C 0 0 0 0 0 1975 Au9 26 7 0 20 20 20 33 20 7 0 0 Nov 13 0 0 0 7 46 0 7 0 198 0 1976 Feb 24 7 0 0 0 0 0 13 0 0 0 May 25 0 0 0 0 0 0 0 0 0 0 Aug 18 40 20 290 99 0 53 92 0 20 0 Nov 0 0 356 13 475 20 139 7 422 13 1977 Feb 24 0 0 7 7 53 508 7 0 7 0 May 17 0 0 0 0 7 0 0 0 0 0 Aug 17 -0 0 0 0 86 7 13 0 172 0 Nov 13 20 59 0 4G 13 46 7 145 0 4 1978 Feb 15 0 13 0 0 0 132 6 6 6 32 May 18 0 0 0 0 0 0 0 0 0 0 Aug 9 0 0 0 6 13 0 0 0 0 0 Nov 14&l5 25 13 0 6 403 38 32 6 19 6 1979 Mar 22 0 0 0 0 0 0 0 0 0 0 May 25 0 0 0 0 0 0 0 0 0 0 .Aug 1 0 0 0 0 0. 0 0 0 0 0 Nov 14 0 0 0 0 0 0 0 0 0 0 1980 ' Feb ' 13 0 0 0 0 0 0 0 0 0 0 May 21 0 0 -0 0 'Sep 23 0 0 0 0 1981. May 12 0 0 7 0 3ep.22 40 90 408 99 1982 May 18 0 0 0 0 Sep 23 0 10 C 0 1983 May 11 20 0 0 0 Sep. 13 59 20 25] 40 1984 May 10 C ~ 0 7 0 Sep 6 0 0 0 0 1985 May -15 0 0 0 0 Sep 19 89 0 99 40 1986 May 13 0-0 0 0 Sep 15&l6 20 20 184 0 1987' May 13 0 10 20 30 Sep 16&l7 30 118 59 99 1988 May 10 0 49 33 30 Sep 13-325 118 92 79 1989 May 23 0 0 39 10 Sep 14 20 118 197 108 (-) indicates area not sampled 126

-. -. -. -. -. ~ ~ - _. - - -. -..- - -- e-DUQUESNE LIGHT COMPANY -1989 ANNUAL ENVIRONMENTAL REPORT 1 Table V-1 6 - suumarizes Corbicula_ present in-ichthyoplankton samples col- ~ lected _ f rom April through August. Only. the night collections (May and July, 1989) contained clams. No Corbicula were collected dur!ng the day -surveys-in-1989. Monthly totalt for Corbicula, collected from the traveling screens during the weekly ' impingement surveys in 1989, are presented in Table V-I-7. The' greatest number of Corbicula were collected in September and Octoberi numb 2rs declined - through the cad of December. Figure V-I-7 presents monthly ' totals fc Corbicula collected during impingement surveys for the years 1981 through 1999. The number of live Corbicula collected from the screens of operating _ intake bays during September 1-15 ranged from 2,380 to 4,612, which was several fold more than the month with the next high-est abundance '(October). Weekly totals of live Corbicula collected dur-ing the impingement surveys frora January -through July numbered six or less. Summary IThe results of - the 1989 Corbicula survey for the Unit 1 cooling tower indicated;that no-live--clams were present in the upper reservoir. Since the water entering _ this area comes directly from the condensors, it is

suspected that elevated water temperatures make this area unsuitable for

- the - :. class.. The -Corbicula-population-in the - lower - reservoir on September 7_ was estimated at 300 million (98% alive). The estimated . population of Corbicula in the: Unit 2 reservoir on March 21 was 18.5 million (42% alive).

=

The river surveys conducted la 1989 ' demonstrate that Corbicula-inhabiting the. upper Ohio drainage provides an extremely large number of _ clams to the ' BVPS. Cleaning of - tne-intake bays by divers resulted L in removing many live clams from the innerbays; this along with-the weekly impinge-ment ' data show that adult-clams move into the plant with the water 1. j: currents. l' !^ l l 127. n 1.

m. TABLE V-I-6 3 Corbicula DENSIT.2S (Clams /100 m ) PRESENT IN ICHTHYOPIANKTON SAMPLES COLLECTED WITH A 0.5m PLANKTON NET IN THE OHIO PJVER, 1988 and 1989 BVPS Sample Location Back Channel Main Channel Date 2B Sur 2B Bot 1 Sur 2 Bot 3 Sur 4 Bot 5 Sur-1988 April 18 0.62 1.96 0 0 0 0 0 5 May 10 0 D 0 0 0 0 0 eO May 11(a) 21.87 18.95 0 0.88 0 7.08 23.00 E5: CC#E June 14 0 0 0 0 0 0 0 "z E" July 14 0.98 0 0 9.24 0 0 0 $C U ge July 14 (a) .0.54 9.09 0 14.75 0 17.86 3.52 5 U E2 n August 17 0 0 0 1.68 0 2.70 2.06 hreE M 13d9 o N April 13 0 0 0 0 0 0 0 May 23 0 0 0 0 0 0 0 May 24 (a) 0.78 6.48 2.08 0 0 0 2.63 June 19 0 0 0 0 0 0 0 July 12 0 0 0 0 0 0 0 (a) July 13 4.84 9.99 4.37 3.38 0 1.67 1.78 August 15 0 0 0 0 0 0 0 (a) Night survey was conducted.

as DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT TABLE V-I-7

SUMMARY

OF Corbicula COLL!rTED DURING IMPIN3DiENT SURVEYS FDR ONE 24-HOUR PERIOC PER WEEK,1989 BVPS Humber Collected Operating Non-Operating Date Intake Bays Intake Bays Month Dy Alive Dead Alive Dead January 6 0 1 0 0 13 1 1 0 0 20 4 3 0 0 27 1 5 0 1 February 3 0 5 0 0 0 3 0 0 10 (a) 17 24 31("I March 3 0 7 0 0 10 0 3 2 3 17 0 0 0 0 0 2 0 0 24 (a) 31 April 7 0 0 0 0 14 0 1 0 2 21 0 0 0 0 28 0 1 0 0 May 5 0 0 0 0 12 3 0 0 0 Ig (b) 26 (b) ~ June 2 0 1 0 i 9 0 3 0 0 16 1 10 0 0 23 0 0 0 0 30 1 0 0 0 July 7 2 1 0 0 14 4 3 0 0 21 6 3 0 3 28 5 8 0 0 I 1 129

. _. -.. ~ DUQUESNE LIGHT COMPANY 1989 ANNUAL DWIRONMENTAL REPCRT TABLE V-I-7 (Cont!nued) o Number Collected Operating Non-Operating Date Intake Bays Intake Bays Month Day Alive Dead Alive Dead August 4 5 12 0 0 11 12 9 0 0 18 50 40 0 0 25 114 29 0 0 September 1 2, 38 0 218 0 0 8 3,351 48 173 4 15 4,612 135 729 24 22 0 0 62 4 29 933 213 122 70 October 6 868 345 544 36 13 227 41 47 17 20 114 . 26 63 15 27 232 134 168 124 3 'DI II November I 10 17 (b) 24 28 31 0 0 December 1(b) 8(c) 15 ICI 22(c) 29 32 31 0 1 'IOTAL 12,986 1,373 1,910 308 I"I (b) Impingemnt could not be et iucted due to high water conditions. Impingement could not be cc u::ted due to diving operations in . seree nhouse. (c) Impingement could not be cosiducted due to maintenance. L t 6 l 130

13000 12,362 O b q $ asco itz 11,253 ow-j k D soo F OO / l U m g eon g 6 M IEE u. 49o. 2o O l 'i CC$$ c $ 200 EI I g t'J + x - L AQ ,h 4 J e ra u J J a s o m o i a p u au J J a s on o l J emau d J a som e l J am au J J a sons lJemau d J asono l JensauJ Ja sono l JeuauJ J a s omo i J emauJ Ja SoWoh J emauJ J a gosD l O s1 82 sa s4 as se gr se es E - DEAD -+- ALIVE r e is 4 8. 4

• • DATA FOR NOVEMBER AND DECEI8BER 1989 REPRESENTS ONLY ONE SAMPLING PERIOD FOR EACH MONTH DUE TO EITHER DIVING OR MAINTENANCE.

FIGURE V-I-7 SUletARY OF Corbicula COLLECTED FROM THE INTAKE STRUCTURE TRAVELING SCREENS DURING IMPINGEMENT SURVEYS, 1981 THROUGH 1989 i BVPS g

DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT 2. Growth Study Objective The.Corbicula grovth study was designed to collect data on the growth rates of clams held in the intake structure and Unit I cooling tower. Methods To calculate growth rates of clams in the Unit 1 cooling tower and the intake structure, clams of known size were housed in 1 ft.2 cages, which were placed in-the study areas. Each cage was constructed of 1 mm mesh fiberglass screening secured within a durable plastic frame and contained approximately ten pounds of industrial glass beads (3/8" diameter) to provide ballast and a uniform substrate for the clams. Shell length (maximum anteroposterior dimension) was measured to the nearest 0.05 mm with Vernier calipers. Cage one was initially part of the 1988 Corbicula growth study, placed _ in the Unit 1 cooling tower on March 3, 1988, and maintained there until the l-September 1989 scheduled outage.- Cage two was placed in the L' nit 1 cool-ing tower on March 22, 1989. Another cage was placed in the intake l structure on March 22, 1989. Clams used in these cages had been removed 1 I from the cooling tower and housed in laboratory aquaria prior to their placement in the intake structure and cooling tower. Initial hell length measurements were made before each cage was placed in its respec-tive location. Clams were selected for each cage ranging in length from. 14.00 mm to 14.90 mm. Growth measurements were made every 28 days until the end of the year. -All clams were removed, shell length measured and recorded, and all individuals were returned to their original cage. An l effort was made to keep each clam out of water for a minimum amount of time. 132

TACLE V-I-8 RESULTS OF Corbiala Get0erm STUDY IN IllTAKE STIIUC9CE AND UNIT 1 CGEING TUtrEBt, 1989 BVPS ' L Intake Structure soling icerer ' Cage 1 Caoe 1 Cage 2 Sam lim Date i e n i e n i s n g no Jan 13(a) 26.11 0 598 39 'Feb 10 26.24 0.603 39 $ E.. 26.51 0.624 39 Mar 10 Mar 22(b) -14.41 0.311 40 14.40 0.303 40 Apr 7 14.42 0.285 40

26. 8b 0.671 39 14.64 0.306 40 y Q.

i May 5 14.43 d.296' 40 27.39 0.690 39 15.41 0.373 40 2~ Jun 2 18.44-0.300 40 -27.80 0.7 32 39 16.51 0.448 40 E"' Jun 30 15.01 0.398 40 27.92 0.743 39 17.60 0.365 40 $C ey Jul 28 17.83 0.673 40 28.11 0.754 79 18.77 0.356-40 8 e-Aug 25 ~20.62 0.648 40 28.17 0.776 39 19.77 0.4:7 40 ~.a. Sep 22 c1 21.87 0.765 40 28.11 0.759 19 19.88 0.508 40 r2 f ICI -- Q 22.36 0.762 40 N 8Ei Oct 20 nov 17(c) 22.36 0.751 40 Dec 15ICI 22.40 . 0.8 95 40 ( E (a) Continuation of cooling tover cage 1, Mareb' 3,1988, with an

• e inital meegurement of f = 14.47.

O +3 + (b) Initial measurement for intake structure enge 1 and cooling tower cage 2. ICI Cooling tower cages removed September 6 due to scheduled outage. (y) mean shell length in millimeters. i (s) standard deviation. .j (n) sample size measured for each sampling date. 4 t I i g

LUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT Results Table V-I-B summarizes the growth data collected from clams in the intake structure and Unit 1 cooling tower. Temperatures were consistently higher in the cooling tower compared to the intake structure. Mean shell length for clams maintained in the intake structure cage increased 6.2 mm from March 22 to August 25 while the cooling tower (cage 2) clams showed an average increase of 5.4 mm in shell leng th for the same period. Growth measurement data could not be collected at Unit I cooling tower for the last three months of 1989 due to a scheduled outage. Summary Le reaults obtained show that growth of Corbicula was initially more rcpid in the cooling tower than in the intake structure, probably due to the higher water temperatures in the cooling tower compared to the intake structure for the conths of March through June. Higher river water tem-0 peratures (typically 70 to 80 F) in late June through August resulted in rapid growth of the intake structure clams during this time. Mean shell ' leng th of intake structure clams on August 25 was 20.62 mm compared to 19.77 mm for the cooling tower (cage 2) clams. 3. Spawning Study Objective The Corbicula spawning. study was designed to collect data on the repro-ductive activity of clams inhabiting the intake structure and Unit 1 cooling tower. Methods Adult clams maintained in cages that were originally placed in the intate structure and Unit 1 cooling tower in March of 1988 were initially sampled (January 13 through March 22, 1989). On March 22, 1989, six cages each containi,9 100 adult clams originally collected from the Unit 1 cooling tower (shell length 217.0 mm) were placed in both the intake structure and Unit 1 cooling tower.

as DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT l Sampling was conducted approximately every fourteen days, at which time, twenty clams were rewoved f rom one of the six cages (cages sampled on a rotating basis) in both cooling tower and intake structure. Samples were transported in a dry towel to the laboratory for examination. In the laboratory, the shell length of each clam was measured to the nearest 0.05 mm with a vernier caliper and recorded. One of the inner gills (demibranch) f rom each clam was removed, dissected, and examined using a dissecting microscope for the presence of pediveliger latvao. The gravid condition of each clam was then recorded using the following criteria Number of larvae Gravid Condition 0 none 1-50 few 51-100 moderate 101-500 many >500 gorged Results The intake structure water temperatures showed an increase through July and declined to December (Figure V-I-8). The Unit 1 cooling tower water temperatures generally increased through July; however, data was not available for the latter months due to the scheduled outage. The Corbicula reproduction study data, expressed as the percentage of clama examined in each gravid condition, also is illustrated in Figure V-I-8 for the intake structure and Unit 1 cooling tower. The greatest percentage of clams examined having pediveliger larvae in the inner gill in the intake structure occurred on August 11, 1989 when 100% were in a gravid condition. The greatest percentage of clams exhi-biting a gorged condition in tue intake structure occurred on August 25, 135

vmTEM TEMPERATURE ('71 100 7-d_ = ..AA_ 40~-

• LHfT 1 CDOLPs0 TOWER - PtTAKE STRUCTt4tE to m,

,,,o ,m .m .n. .n am.,. .r. an .m em vm rm .r,. en. .m .n. m m,,,,,, Cm oes % CLAWS WITH PEDtVEUGER LAR'iAE UNIT 1 COOLING TOWER E K So-to so - =# ~ EO 40-z OUTAGE 1 's te [ $E

• " ~

l gg U o' = y,, y,7 ,m in. sm an 4,7 .m m.. .n .m .no rm rn. .m .n. .n, ,o,. ,ono ,u ,m7 u,, o,,.,,,,. 5e hO % CLAWS WTTH PEDtVEuoER LARME

ig soo -

INTAKE d MUGIORE E t ~ g4 .I 3 so-x e .e eo-i--, a 4o-R Q v + ~ g p vis Vrf im 2t94 8/10 8/22 4/7 4/f, St. .m e/3 sti. erso 7tts 7tt. sitt afs. ses eras 10/. scisu 'ut 11m tan n/1. e o GRAVID CONDITION l I E GORGED E MANY ~ ' MODERATE C FEW i FIGURE V-I-8 RESULTS OF Corbicula REPRODUC1' ION STUDY IN THE INTAKE STRUCTURE AND UNIT 1 COOLING 'IDWER, 1989

DUQUESNE LIC11T COMPANY 1989 ANNUAL ENVIRONMENTAL RCPORT 1989 when 60% of the clams examined were incubating more than 500 pedi-veliger larvae in the dissected gill. Overall, the advent of pediveliger larvae release was continuous from June 30 through September 8,1989, and perhaps to the following week. The availability of larval clams through the intake structure occurred more or less continuously for 10 to 12 weeks in 1989. In the cooling tower, the only time that clams were in a gravid condition occurred on August 25, 1989, when 70% of the clams sampled from the cool-ing tower cage were incubating larvae. None of the sampled clams exhibited a gorged condition in the cooling tower. From September through the end of the year reproduction data could not be collected at the cooling tower due to a scheduled outage. Summary The period of potential larval release from gravid adult clams occurred in late June to mid-September 1989 at the intake structure. Unit 1 cool-ing tower clams had a minor larval release period in the latter part of August. ~ 4. Larvae Study l g et_t,iy The Cy p,1cula larvae study was designed to collect data on spawning activitiee in the Ohio River and BVPS Units 1 and 2 cooling towers. Methods Empty clam cages (larval cages) were placed inside the intake structure and cooling towers. For every month of the initial five months, an empty clam cage was placed in each cooling tower and two cages were placed in the intake structure. This resulted in the placement of five clam cages l in each cooling tower and ten cages in the intake structure. The clam l cage mesh size (1 mm) permitted only very small clams or pediveliger l 137

~ _ I s. + l'. DUQUESNE LIGHT COMPANY A989 ANNUAL ENVIRONMENTAL F" ? ORT larvae to enter and colonize.he cage. Removal of a clam cage for exam- ) ination of live and dead clams occurred five months af ter initial place-l ment. Each removed clam cage was returned to the laboratory where it was washed to obtain the clams which had colonized the cage. Corbicula obtained from each cage were rinsed through a series of stacked sieves ) with mesh size ranging from 16.00 mm to 600 microns. The 1 rgest and smallest live clams were measured using the Vernier calipers to establish a range for ti a sample. It should be noted that the size distribution data obtained using the sieves reflects clam width, rather than length. Clama retained on each sieve were counted and the number recorded. Results The larval sampling cages that received the most Corbicula were located in the intake structure (Table V-I-9). The g.eatest number of clams (alive and dead) collected from one cage was 2,491 (July 13 to December 15). The largest clam measured (20.10 mm) was obtained f rom the intake structure (sample period May 12 to October 13). Table V-I-10 and Figure . - V-I-9 present size distribution data for clams collected in the larval sampling cages. The intake structure graph presents size distribution data representing an average for the two cages which were sampled each month. The influx of juvenile clams ( $ 1.0 mm) into the intake structure cages was not observed until August 18, when ambient river water temperatures were in the 75 to 80 F range (Figure V-I-9). This delay in colonization inay have resulted from high water conditions in the spring which inter-rupted a bimodal pattern of larval releases that was obsteved from the 1988 survey. June 16 showed the first time.arge numbers appeared in the Unit 1 cooling tower cages. The first larval study cage examined af ter the Unit 2 outage and recolonization period (October 13) contained numer-ous clams that were retained on the 3.35 ma sieve (408) and 6.3 mm sieve a (69). This would suggest that spawning had occurred several months earlier during the recolonization period. The interruption 1.n larval cage study data for Units 1 and 2 cooling towers due to scheduled outages linits the interpretation of results and comparisons between these BVPS structures and the intake structure. 138 -mer

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x m s TABLE V-I-10 RESULT 3 OF THE Corbicula, LARVAE STOCT SI2Z DISTRIBJTICM IN TEE INTAKE savvivRE AND UNIIS 1 AND 2 CDCLING TONEPS,1989 BVPS Liv ulam Size Distribution Numbers Tocal Cage Date Location 51.00 (nel 3.35(m.) 6.3(mm) 9.5fami

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G- - l TABLE V-I-lO' (Continued) i Cage Live Clan Size Distribution Numbers _ Total 'Dete Zecation <1.00 fuel ' 3.35 tan) 6.3 tan) 9.5 tan) 12.S tami 16.0tas)_ L* e Clems/ Cage l Aug'18 Ist 282 10 0 1 1 0 294 1 ct('b). 21 11 37 8 0 0 77 2 ct E Sep 15 Int 185 563 123 4 8 0 886 .., E I 6 1 ct *I su 43 97 0 0 0 200

gg j

15 2 ct(b) E: C Oct 13 Ir' 76 369 240 12 2 0 699 Z ct "I h I ~C 2 et 439 488 39 0 0 0 1,016 8k I lO ^ Nov 10 Int 117 397 433 21 0 0 958 E 1 et *I '"i 2 et-217 046 297 0 0 0 9 t.; D Dec 15 Int 514 1,459 392 6 0 0 2,371 g2 1 et *' T I 2 ct 142 101 348 0 0 0 791 O

• a Totals 2,835 4,463 2,082 55 11 0

9,447 i I*I ' cooling tower cages removed due to' scheduled octoge. M Cage placement for clas recalonization. ICI Number of clams represent the average of two ceges in-the intake structure. Symbols l I Int - Intake structure 1 et - Unit 1 cooling tower 2 et - Unit 2 cooling tower 5

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DUQUESNE LIGitT COH1'At1Y 1909 ANNtlAL EINIRONMEITTAL REFORT Water temperatures in both cooling towers typically fluctuated note from week to week than the intake structure (Ohio River) water temperatures (Figure V-I-9). This along with chlorination probably influences repro-ductive activity and latval survival of clams in the cooling towers above I any other env.ronmental factore. Summary Results of the larval cage study for the intake structure indicated that spawning activity in the Ohio River occurred from August through December of 1989. Chlorination of the cooling tower water may be a factor in the larvae study results. / 1 143

-~ . - _. - _ - _~ -.- DUQUESNE LIGHT COMPANY 1989 ANNUAL ENVIRONMENTAL REPORT VI. REFERENCES

Burch, J.

Q., 1944. Checklist of West American Halluske.

Minutes, Conchology Club of Southern California 38:18.

Commonwealth of Pennsylvania, 1985. Pennsylvania's Endangered Fiches, I Reptiles and Amphibians. Published by the Pennsylvania Fish Commis-sion. Counts, C. C. III, 1985. Distribution of Corbicula fluminea at Nuclear Facilitiot. Division of Engineering, U. S. Nuclear Regulatory Com- ) mission. NUREGLCR. 4233. 79 pp.

Dahlberg, M.

D. and E. P. Oduc, 1970. Annual cycles of species occur-r etx:e, abundance and diversity in Georgia estuarine fish popula-tions. Am. Midl. Nat. 03:382-392. DLC, 1976. Annual Environmental Report, Hon-radiological Volume 81. Duquesne Light Company, Beaver Valley Power Station. 132 pp. DLC, 1977. Annual k.../ir onmenta l Report, Non-radiological Volume fl. Duquesne Light Company, Beaver Valley Power Station. 123 pp. DLC, 1979. Annual Environmental Repott, Non-radiological Volume fl. Daquesne Light Company, Beaver Valley Power Station. 149 w. DLC, 1980. Annual Environmental Report, Non-radiological. Duque sne Light Ccmpany, Be. aver Va1. ley Power Station, Unit No.1.160 pp. nLC, 1981. Annual Environmental Leport, Non-radiological. Duque3ne Light company, Beaver Valley Power Station, Unit No. 1. 105 pp. + Appendice s. DIC, 1982. Annual.invironmental Report, Non-radiological. Duquesne Light Company, be aver Valley Power Station, Unit No.1. 126 pp. DLC, 1983. Annual Environental Report, Hon-radiological. Duquesne Light Company, U* aver valley Power Station, Unit No. 1. 124 pp. + Appendix. DIE, 1984. Annual Environmental Report, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit ho.1. 139 pp. DLC, 1985. Annual Environmental Report, Non-radiologierl. Duquesne Light Company, Beaver Valley Power Station, Unit No.1 & 2. 106 pp. DI4, 1986. Annual Environmental Report, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit No.1 & 2. 152 pp. DIf, 1987. Annual Environmental Report, Non-radiological. Duquesne Light Company, Beaver Valley Power Station, Unit No. 1 & 2. 145 pp. 144

f D'JOUESHE LIGilT COMPANY 1969 ANNUAL ENVIR0!!H13(TAL REPORT DLC, 1988. Annual Environmental Report, Non-radiological. Duquesne Light Company, Ber.ver Valley Power Station, Unit No.1 & 2. 161 pp.

Gilbert, C.

R., 1964. In: A List of Common and Scientific Names of Fishes irom tt'e Unled States and Canada, 3 rd Ed. 1970. American Fisheries Publ. No. 6. Waahington, D.C. Hutchinson, G. E., 1967. A treatise on limnology. Vol. 2, Introduction to lake biology and the limnoplankton. John Wiley and Sons, fnc., law York. 1115 pp.

Hynes, H.

D. H., 197D. The ecology of running waters. Univ. Toronto Press, Toronto.

Jenkins, 11arold and Frank Logar, (Dic Operations Per sonne.?,

DVPS) perscnal communication, December 3,1985. NRC, IE Bt:11etin 01-03: Flow Diockage of Cooling $4ater to Safety System Components by Corbicula op. (Asiatic Clam) and Mytilus sp. (Mussel).

Pielou, E.

C., 1969. An introduction to mathematical ecology. Wiley Interscience, Wiley & Sons, New York, NY. Robins, C. R., R. M. Bailey, C. E,, Bond, J. R. Brooker, E. A. Lachner, R. N. Lea, and W. B. Scott, 1980. A list of common and scientific names of fishes f tom the Unite / tes and Canada (Fourth edition). Amer. Fi sh. Sco. Spec. Publ. M 1.-174.

Winner, J.

M., 1975. Zooplankton. g: B. A.

Whitton, ed.

River ecology. Univ. Calif. Press, Berkeley and Los Angeles. pp. 155-169. 4 145 l'}}