View source for AEP-NRC-2009-32, Enclosure to AEP-NRC-2009-32 - Donald C. Cook, Units 1 and 2 - 2008 Annual Environmental Operating Report, Appendix V Through End

{{Adams
| number = ML091340525
| issue date = 04/29/2009
| title = Enclosure to AEP-NRC-2009-32 - Donald C. Cook, Units 1 and 2 - 2008 Annual Environmental Operating Report, Appendix V Through End
| author name = 
| author affiliation = Indiana Michigan Power Co
| addressee name = 
| addressee affiliation = NRC/NRR
| docket = 05000315, 05000316
| license number = DPR-058, DPR-074
| contact person = 
| case reference number = AEP-NRC-2009-32, FOIA/PA-2010-0209
| document type = Annual Operating Report, Environmental Monitoring Report
| page count = 245
}}

=Text=
{{#Wiki_filter:APPENDIX V SPECIAL REPORTS 2008

August 15, 2008 EPA Acceptance of Supplemental Environmental Project

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 5 77 WEST JACKSON BOULEVARD 4o                                             CHICAGO, IL 60604-3590 REPLY TO THE ATTENTION OF:
SC-6J CERTIFIED MAIL RETURN RECEIPT REQUESTED Lawrence J. Weber, Site Vice President Indiana Michigan Power Company One Cook Place Bridgman, MI 49106 Re:      Indiana Michigan Power Company, Bridgman, Michigan- Consent Agreementand Final Order Docket No.: CERCLA-05-2004-0010, EPCRA-05-2004-0043, MM-05-2004-0003

==Dear Mr. Weber:==

The U.S. Environmental Protection Agency (EPA) had received and evaluated the Supplemental Environmental Project (SEP) completion report from Indiana Michigan Power Company. This review compared the SEP completion report to the Consent Agreement and Final Order. Based on the terms of the CAFO and the information that you have provided, the EPA accepts the SEP completion report. Indiana Michigan Power Company has completed the SEP and the SEP completion report as per the terms of the CAFO.
If you have any questions or concerns about this matter please contact James Entzminger at (312) 886-4062. If you have any legal questions, please contact Richard Wagner, Associate Regional Counsel, at (312) 886-7947. Thank you for your assistance in resolving this matter.
Sincerely yours, ark J. Horwitz, Cief Chemical Emerge /cy Preparedness and Prevention Section cc:      Richard Wagner (C-14J)
Kevin D. Mack, Esq.
American Electric Power 1 Riverside Plaza Columbus, OH 43215-2373 (certified)
Recycled/Recyclable . Printed with Vegetable Oil Based Inks on 100% Recycled Paper (50% Postconsumer)

February, 2008 Mexel Efficiency Study

                                          &#xfd;MINICHIGA Aunit ol'Aimerui V,nEecticov 4 a'
olloe.;06 f. t :0.
A *.--4 Final Repor Februafy 2008

TABLE OF CONTENTS REPORT INTRODUCTION ....... ..                                              ......                   ;..........*.            ....                           *.      .....                            PAGE ABSTRACT..                                                                     . . . . . . . . ......................                                     ........               ...................
IN T R O D U C T ION ..............................................................................................................................                              *.............2 .
BACKGROUND          ......................               ....................
ME T HO D S .. .. . ....... .................... .. . . ... ................. .. . .. . .-. .. . . . .. .. . . . .................. ...... *......... .. '....
                                                                                                                                                                                        ':           . ............. 12 METHODS...................                                           .        ............                           .                    .             .            .            .           .
REPORT RESUfLTS                ........                ..................                           .................                          .      .         ..         ...............................
ZEBRA M USSEL SAM PLING BIb .BOX DATA ..... ............... ...................................................................................                                                                7 ARTIFICIAL SUBSTRATEANALYSIS (CARBON STEEL METALCOUPONS).v. ........ 1......                                                                                                        ..             .           9 ARTIFICIAL SUBSTRATE ANALYSIS (PLEXIGLASSBAFFLE PLATES) .                                                                                             .. .........                                            10 TOTAL MUSSEL DEBRIS'COLLECTION /QUANTIFICATION ................................                                               .                                                                               1.1
:- . .CORROSION  RATE EV.ALUATION........                                       ........... 11.....                v. ......                                           ............................13 INTERNAL IN"VESTIGATION AND OBSERVATIONS&.........-..............                                                             ........       ......
                                                                                                                                                          ..................................                         1.16
                                                                          .................                        I .......            ..
TOTAL SUSPENDEDSOLIDS SLO GHOE RA E ...............     .........            .........                     :...........................                      ................................. ..... 17 M EXEL M ORTALITY EXPERIM ENT ................................                                                   .................         .......................... .................
REVERSE OSMOSIS MEMBRANES ...;-;................ .................                                                                                       ........                         .......... IS O RG A N IC LO A D IN G .......... ................................................................................................................                                  ;      . .............49 WHOLE EFFLUENT TOXINCITY TESTING 

==SUMMARY==
(WET TEST) ............                                                                                                         ........         ...... ....        20&#xfd; REPORT CONCLUSIONS:...................                                                                                      .......
CONCLUSION&S..........                      ......     . ......................
                                                                                                                                            ............                                                              23.
ACKNOWLEDGEMENTS ....                              .        ..................................................................                              . ........            ..............
                                                                                                                                                                                         .....                       24 REFERENCES&.........................................                                                    .........
                                                                                                                                                                                                 ......    ... 2.
APPENDICES.......... ....................                       .....................                 ...        ..................                                                      ..............
...-    APPENDIX.] -IMPACT STUDY OF MEXEL WITH CONTINUOUSFLOW RESEARCH:FACILIT..                                                                                                                                ......
1 APPENDIX 2- CNP PROCEDURE I 2-EA6o09 6AE N -I101 ZEBR4f MUSSEL SAMPLING AND ANALYSIS,.
APPENDIX 3- GLEC-WET TEST RESULTS AND CNP ZEBRA MUSSEL ANALYTICAL RESULTS .......
APPENDIX 4-MIXING ZONE'EVALUATION; ..                                                       .......................-....                                        w.......
APPENDIX 5-    5AST*M-PROCEDURE FOR WATER CORROSIVITY BY WEIGHT LOSS .....................................
APPENDIX 6 -AVISTA MEMBRANE AUTOPSY REPORT.;..                                                                                            .........................
APPENDIX 7- H-'O`-H WATER TECHNOLOGYANALYTICAL RESULTS .........................
APPENDIX 8- ZEBRA MUSSEL MONITORING AND CONTROL ASSESSMENT REPORT;..,.;..-..... ...
APPENDIX 9WWATER & POWER TECHNOLOGIES - TECHNICAL ANALYSIS REPORT ....................

A             Mexel Efficiency Study DC Cook.Nuclear Plant Final Report February, 2008
                'Thomas Armon, Darius Barkauskas, Jon J. Cohen, EriciC. Mallen Abstract Mexel, a chemical. product-in the general classification of filming amines, has been'evaluated foir use as a Oreveitive molluscicide control.program at- AEP's Indiana Michigan Power.
Company Donald C. Cook Nuclear Power Plant, Bridgm*an Michigan (CNP). Mexel is marketed as. a corrosion inhibitor, dispersant and control agent for cooling water systeimi fouling species. such as. muss~.1s and hydroids. A unique on-site research rfacility was constructed and operated' continuously for 365 days to, evaluate Mexel efficiency in preventing: zebra mussel infestation on cooliag Water intake tunnels at CNP. Stiandard and custom testing methods-were used to determine the performance of Mexel on modeled intake tunnels using natural popuVlations of zebra mussel trans-Iocatorsm. and larvae under dynamic conditions.
The findings~indicate that a Mexel product dosage regimen of 4 ppm, for 40 minutes/day illustrated:
* Effectiveness in preventing infestation of zebra mussel colonies in: corrugated pipes patterned after CNP intake tunnels.
Reduced silt and sludge accumulation in flowing water circuits.
* No degenerative fouling of reverse osmosis membranes.,
No rapid mussel detachment (sloughage) :of existing colonies from tunnel surfaces.
* Minimal increase in organic loading of treated water circuits or receiving waters.
     * .No negatiVe impact on Great Lakes fisheries, aquatic life or wildlife when discharged, un-neutralized into Lake NMichigan as measured by whole effluent toxiAcil tests.
Fina!Repodrt - FebruAry 008                                          Mexel Efficiency Study Page 1 of 24              DC Cook Nuclear Plant 35

==
Introduction:==
CNP has: dealt with zebra mussel infestation since 1990 and. has employed many different treatment alternatives Three intake tunnels constructed 'of 16-foot diameter corrugated, galvanized steel pipe extend about 2,250 ft. from a comimon forebay. Corrugations in this pipe are 6 inches wide (peak to peak) and '2-inches deep. Flow patterns enable. zebra mussel attachment to,the tunnels typically in the top: and downstream side of the corrugations. Once zebra&#xfd; mussels have attached to the tunnels,, they populate and grow as individuals or in "clumps"' as they attach .to one another.
Zebra mussels can accumulate to a thickness of two to four inches during peiods of -reduced flow butare typically limited Ito one to two inches at normal velocity, This is dueto-mussels sloughing off the tunnel w6lls whenrthe.-mussel layers exceed 2 iniches. Thewater velocity -in the intake tunnels is 6 to7 fps, strong enough to carry clumps of detached mussels into the intake forebay. Mussels in these clumps-will either-reattach to0 the intake forebay walls or be gathered by the traveling water screens. When sloughing rates are naturally high   lor following shock 'treatments (treatments designed'to kill 'all accumulated mussel populations within a 2 to 4 day period), the traveling Water screens and'the trash removal system are challenged to remove the mussel debris at the same: rate, at 'which the debris enters. the. intake forebay. The-traveling screens have been overcome by large influxes of debris as. washing operations.
require traveling screen shutdown to allow.the trash collection baskets to be emiptied and fork.
lifted' to 20: cubic yard dumpsters for removal by a waste hauler off site. The clay 'used to detoxify shock treatment: biocides has resulted in plugging of small bore piping systems d6whstream of the traveling screens. The "effect:ofthese difficulties is, degraded operation of CNP's 'cooling water system.,
To reduce this risk, CNPreplaced the flow through traveling water screens with multi-disc6 traveling water screensin 2004,4and upgraded the screen' wash systdem to handle, higher-trash and shell debris loading rates.-in addition, the new 'screens preclude 'all carry over debris.
These 'improvements have reduced the challenges posed- by mussel debris: but have not eliminated the problem. The sloughage of shells can&#xfd; potentially block flow in the safety related service water systems. Given this' challenge, CNP has&#xfd; continued ,to.search for'a zebra mussel preventive control program that does not require a shock feed cycle, prevents infestation, does not cause rapid sloughage Of 'existing populations, and does not require detoxification for safe discharge into Lake Michigan, Mexel was chosen for careful evaluation as a Water treatment additive under standard and. cuSto techniques described later In.this report.

===Background===
Mexel is a, proprietary molluscicide that has been used in freshwater and saltwater:systems worldwide. Kreuser. et al, (1997), wrote a review of the efficacy of Mexel in fresh iad salt water cooling systems. Mexel is a-"filming annine which,' when properly applied to a cooling system, forms a film on system surfaces that is believed to prevent zebra'mussel, settlement as the control mechanism 'rather' than creating a toxic water column as the controlling effect.
Toxicological effects of Mexel have been widely studied in both freshwater and saltwater.
(Ghillebaert; 1997 & McCaulley, 2005), Biodegradation of Mexel was also demonstrated and documented. However, prior to this study there had been little published. informatioh.
concerning the effect of Mexel on: existing zebra mussel infestations on 'corrugated pipe in freshwater applications. InformationT had been especially -limited regarding the application o6f Mexel on the CNP intake tunnels, Lake Michigan water, and the removal of a previously established population.
Final Report- February 2008                                          Mexel Effiqieiicy.Study Page 2 of 24             DC Cook Nuclear Plant

Power plants are complex industrial facilities that contain integrated components constructed of different materials performing a variety of functions. Any chemical product added at the intake must be compatible with all materials of construction that treated water contacts in the plant. For example, membranes in the reverse osmosis (R/O) units are susceptible to contamination by complex organic molecules. In part, the design of this study was to provide more information regarding Mexel's compatibility with plant systems and impact on corrosivity.
To provide robust modeling data, this study was designed using a modular custom fabricated continuous flow research test rig. The goal was to design and safely operate a model that assimilated tunnel conditions without interrupting normal plant operations. Continuous flow research facilities have been used to model effectiveness of molluscicide control programs on once through cooling water systems, (Ackerman/Claudi, 1994). Modular flow-through design using natural populations have previously provided robust data that enabled treatment modeling for plant systems under dynamic conditions.
Once installed, the rig was operated for 365 consecutive days to ensure accurate representation of a complete growing and larval season, fluctuating water temperatures, and dynamic silt loadings. In summary, the pilot test rig experienced a contiguous year of the naturally variable conditions imparted by Lake Michigan on CNP.
Images of the Modular Test Rip Final Report - February 2008                                          Mexel Efficiency Study Page 3 of 24               DC Cook Nuclear Plant 6

Methods I. Design The design Specifications, as. built diagram, and scopeof the&#xfd; modular apparatus can*be found in Appendix: I. Briefly,, the apparatus was constructed with three separate flow trains of. corrugated pipes ,to; simulate the corrugation of the intake tunnels. One pipe was used as a control, receiving only untreated lake water at flow velocities comparable to thosei in'the intake tunnels. A second pipe operating at the same velocity as the control was treated with the daily Mexel dosage projected for full scale tunnel-treatment. The third pipe was operated at a lower velocity to enable zebra&#xfd; mussel growth and. colonization without rapidly, moving water streams:. It is believed, the velocity within the third section accurately models the velocity: within the tunnel corrugation trough bottoms.. :Sections of thfe&#xfd; growth pipes were oalso used to study Mexel's effect on existing infestations to, conmparatively evaluate sloughage under the normal dosage regimen. To acceleratethe fouling process in the growth section, several handfuls of live musSels were collected from the traveling screen trash baskets and loaded 'into this section.
I. Chemical and Biological Evaluations By Standard Procedures:
: 1. The efficacy of Mexel was studied using CNP procedure 12,EA-6090-ENV-101 Zebra Mussel Sampling and Analysis, found in, Appendix 2 attached. Results of these procedures can be found attached under Appendix, 3. This procedure is! based' on "Standard, Protocols for Monitoring, and Sampling Zebra Mussels'" by J. Ellen Marsden, Illinois NaturalHistory Survey 1992.
: 2. The method :used for determining safe, Mexel discharge concentration to Lake Michigan is based on "Mixing Zone Evaluation" by D.J. McCauley of GLEC, 2005. found in Appendix 4. Whole effluent toxicity (WET) testing was performned by GLEC in accordance with EPA/600/4-90/027 and EPA-82 1-Rm02-012.
: 3. Corrosion evaluation wasperformed in accordance with the Annual book of ASTM Standards. Section 11' Water. & Environmental Procedure D26889'StandardTest Methodfor Water Corrvosivity by Weight Loss found in Appendix 5,attached.
: 4. Water analyses were performed iri.accordance with Standard Methodsfor the Examination of Water and Wastewater 20th Edition.\
: 5. Total Suspended Solidsr residue: was also performed &#xfd;in accordance with Standard Methods for the Examination of Water and Wastewater 20th Edition.
Final Report - February 2008                                          MexeLdEfficiency Study Page 4 of 24             DCCook Nuclea-rPlant

III. Custom Techniques for Evaluation
: 1. The test rig was.1 co~nstructed of three stainless steel corrugated pipe sections. Flow was& controlled by throttling. valve. position and flow shunting through the stantd-by water delivery pump installed in parallel to the main delivery pump. Flow velocity Was confirmed. by both paddle wheel and magnetic. type, flow meters. At the surface in the tunnels the velocities are much lower (I to 2 fps). Eddies created by the corrugations allows larval and juvenile mussels to settle on the downstream side of the corrugations (Zebra Mussel monitoring and control agsessment report
                     #CR-033440!3 Appendix 8),
The test rig nodeled the teducedvelocity at thetunnel surface and in the troughs by two-techniques;
: i. installed ig pipe.. corrugations ii. The velocity (1 to2 fps) of the-third section (grbWth).,
2 BoroscopJc inspections of the. test rig corrugated pipe, sections. were performed monthly: to. progressively evaluate settlement control and. to compare with previous remote&#xfd; operated..vehicle inspections (ROV) of.the, maintunnels.
               .3. Artificial substrates. were deployed and analyzed. under Standard Procedurel above, The artificial substrate analysis included carbon steel, metal specimen- corrosion' coupons installed in a controlled velocity test rack. Plexiglass, baffle. plates installed in the bioboxes to produce more uniform flow patterns were also treated as artificial sub&#xfd;strates and used to
                    'estimate zebrao mussel accumulation rates. After-the study was complete
                   '(2) 1-inch scrapings Were taken from each baffle plate and analyzed using the sameprocedure for the slides and coupons.
: 4. To quantify and compare total settlement during the study; a simple collection procedure was, developed. The middle pipe section of each flow.v train! (treated, control, and growth)was sayed post project and sealed to preserve collected shell and debris loads. The pipes~were pow'er Washedl at 2,200 psi a&#xfd;d ar ticulated to ensure removal fromtrough corrugations, The,
                   *water .slurry (debris loaded wash water) was. collected for.. filtration through coarse mesh filter spcreens. The: separated solids were photographed, transferred to a storage pail, dried and then weigfhed to determine. the amount. of debris that was collected !in each flow cell. The
                    .relative, quantities were used to extrapolate the treated reduction. of shell debris at the studied treatment regime.
Final Report - February 2008.                                           Mexel EffiiencyStudy Page 5 of 24            DC Cook Nuclear Plant

               '5. Given the risk. that rapid deihfestatiodn upon initial fil scale, Mexel treatments could challenge the screen house, two custom techniques were employed. To understand Mexel's impact on existing populations, filter baskets were ;added to capture mussel sloughage. detaching- from. the surface, of both treated and untreated pipe sections., Each week the baskets.
were removed from the flow streams,, observed, cleaned, and placed back in. service. The Volume and mass of mussel debris collected in the baskets was assumed to be an estimate of the: relative debris in :treated vs.
untreated intake, tunnels. Flow rate indicators, were used to quantify consistent volume of flow. Comparative photographic inspections- were used &#xfd;to judge rates of sloughage and overall system cleanliness. Relative mussel sloughage rates by' Mexel were made with this 'technique by comparative visual observation of the filter baskets.
: 6. A Mexel mortality, experiment Was devised to further understand the impact Mexel would. have on healthy colonies. it is believed that a, more rapid "die, off' of healthy mussels treated with Mexel would model a rapid detachment .of healthy colonies in the tunnels at full scale: treatment. Two hundred live healthy zebra mussels collected from screen house, trash baskets were loaded into separate, stainless steel wire cages, one for the treated ;and one&#xfd; for the control biobox. The treated. biobox received its normal daily dosage of Mexel while: the: control, received untreated lake water. Each weelk mortality was, evaluated by counting the numbrers of surviving mussels in the treated cage and the control cage.
               -7. To, evaluate the impact on the. make-up plant 'system, a model reverse osmosis (R/O) system was placed on the treated flow stream.a The R/O Was.
operated continuously and received untreated.. Lake Michigan: water, as its make-up &#xfd;source for.23 hours 20 minutes :per day, and for the final 40 minfites the R/O received- Lake Michigan water treated with Mexel at thee studied dosage regimen. The: RIO membranes treated With Mexel were autopsied by H-O-H pro:cedure:#RO! 23 and compared against autopsies&#xfd; from the Avista Technologies membrane autopsy report: (Appendix 6) from July 2003, Which had. been performed to evaluate'the impact of GE Spectrus CT1300 (a competing chemical additive product), on fouled R/O miembranes.
8., To measure the impact ,of rganic, loading that a preventive treatment approach would add t' the CNP Water distribution system aswell as Lake, Michigan,, total, organic. carbon and total 'organic nitrogen were analyzed.
Organic loading iinparts unintended deleterious' effects on water. systems such as microbial. contamination and growth as well as unwanted sediment. Grab samples were collected weekly: from the test rig smfiple ports and analyses for TOC, 'TON, and general. water chemistry, were performed at H-O-H Chemicals' laboratories in Palatine, IL, Final Report - February 2008                                          Mexel Efficiec'4y Study Page 6:of 24             DC Cook Nuclear Plant

Results::
The analytical data gathered during thei study are in Appendices 3 and 7. This includes water and corrosion analyses: performed by H-O-H laboratories, in Palatine IL, who le effI uent toxicity testing by GLEC Labiorgatries, Traverse City M!, density and- size evaluation by CNP personnel,: collection of data from online instrumentation, field testing, and' custom techniques by: CNP and H-0-H personnel. Theo following, is a graphic 'illustrafion of the
 .results accompanied by writtenr interpretation.
Zebra Mussel Sampling-Biobox Data Post-&#xfd;veliger biobox data for zebra mussel population density and, average size,:as&sect; measured by CNP procedure: 12-EA-6090IENV-Il          0Zebra Mussel Sampling and Analysis protocol are shown in Table. I 'for those slides exposed during the experiment. The table shows the mean value of the population density as well as:the standard, deviation for each sampling date, The final set ofslides which were exposed for one .full year is shown graphically in Figure 1.
Tabled    -Post-Veliger     Biobox Data
:Control                                       ;Treated, Mean"Post-Veliger         standard           Mean Post-Veliger          Stndard Sa-mple Date      Population Density                           Population Density         Deviation (Number/m2)             Deviation              (Number/mn 2
                                                                                    ).
September !13; 2006                   427                  754                    1;067'                 533 7September 28, 2006t                .1,1731                 911                     1,813:              1,917
'October 12,2006                     2,560;               3;0961                  14,933               19,827 October,25,'2006                    8,960-               4,453.                  13,653               106966f November 9, 2006, .               40,107                 8,057'                  28,907              18,456 December.7,2006                   85,440               40,100                    60,800               12,672.
June 7, 2007                     108,978,               17,804                   39;289                4,473 August 23; 2007                  553,600              125,2051                  131,947.             41,204:
The standard deviation was determined by calculating the mean value0of each Sample date.
The' sqiuared4 difference of, ea*h sample from the mean valvie was then calculated.;, The average of the squared difference is the variance of the,-sample date. The standard deviation is the square root"of the variance. This is iIlustrated &#xfd;in Equations I and 2.below.
Equation (1): Sample Mean of Sample Date Where:
Sample Mean= x = " Ex.
e (.) is thernean of the sample date
                                                           * (N)'is the numbers of samples on the sample date
                                                           * (4j) Is the&#xfd; sample value Ecuation.(2): Standard Deviation Where:
StandardDeviation-*r=,-             .(x1 - X)r                (a)'is theJ'standard-deviation Ni=I"
                                                           * (N),.is the numbers of'samples on the
                                                                'sample date
                                                            * (xd is the sma-ple value,
                                                            .    (x,),is the mean of the sample date.
Final Report - February 2008'                                                 Mexel Efficiency Study
                                               'Page 7 of 24                  DC Cook Nuclear Plant,

Initial data from September 13, 2006 to October 25, 2006 suggests that the control slides were performing better than the treated slides. The November 9, 2006 sampling marked the first time when the treated slides were performing better than the control slides. During this same sampling date an independent observer noticed that water flow in the bioboxes was potentially short-circuiting and not getting to the surface of the slides. The observer recommended installing baffle plates to redirect the flow to the bottom of the biobox to prevent the short circuiting and direct the flow across the slides. The biobox baffle plates were installed on November 16, 2006. (See Figures 3 & 4 for baffle plate images.) All subsequent sampling dates showed a dramatic improvement in the treated slide data. This suggests that Mexel performs best when it is allowed to reach the surface of the test substrate.
AEP, Donald C. Cook Nuclear Plant H-O-H A-432 (Mexel) Testing Post-Veliger Density & Average Post-Vellger Size Control vs. Treated Population Reduction - 76n                             Avefa* Siz Reduction - 46%
60,000                                                     2,500    .....       ....             . ... . .
I,   500.000
                                                                                                               *i~r De79Oion Cotrl-W            lu
     >,400.000 Ta* Conro
                                                -- -j)#1S        500 12s 41,00.0.,  1000o a     300&deg;000 500 200,000
  >    100.000  i                           ,.ent                      - -
Crnultit-Ye Settset Currudetlvt I-Yewn Setttemnent I     &#xfd;&#xfd;                               &#xfd;7441"&#xfd; Figure 1 - Post- Veliger Density, Treated vs. Control (Glass Microscope Slides)
Figure 1 illustrates Mexel treated and control biobox slides. Cumulative settlement (1 year) population density was reduced by 76% as compared to the control group. Average post-veliger size, also illustrated in Figure 1, indicates a reduction in size by a cumulative average of 46%. The population and size reduction shown is based on an average of 5 slides in each biobox that were exposed for one year.
Final Report - February 2008                                                              Mexel Efficiency Study Page 8 of 24                    DC Cook Nuclear Plant ko

Artificial Substrate Analysis (Carbon Steel Metal Coupons)
Metal coupon strips were also used to determine post-veliger density and size by CNP procedure 12-EA-6090-ENV-101 Zebra Mussel Sampling and Analysis protocol and are shown graphically in Figure 2. Standard corrosion coupon racks, built to ASTM standards, were incorporated into both treated and control flow trains. Flow velocities were also controlled accurately through Dole flow control valves designed to maintain consistent velocity (6.0 ft/sec) through these assemblies.
AEP, Donald C. Cook Nuclear Plant H-0-H A-432 (Mexel) Testine Post-Veliger Density & Average Post-Velilger Size from Steel Coupons Control vs. Treated Population Reduction.- 77t6           1200 Average Size Reduction A SSO.000                                 -          ----
1.000 ContStadar                                 I      Deviat8ion8~
250.000                                                                                     Tr ol * (Te
                                                                                                          -) 432 pan 400 I                                 '                    20*
Cumulative (S6 Day) Settlement Cumulative (56Da)settlement U Conro          Treated Figure 2 - Post-Veliger Density, Treated vs. Control (Steel Coupons)
Figure 2 illustrates Mexel treated and control corrosion coupons. Cumulative settlement (56 days) population densities were reduced by 77% as compared to the control group. Average post-veliger size, also illustrated in Figure 2, indicates a reduction in size by a cumulative average of 66% as measured on metal coupons. The population and size reduction shown is based on two carbon steel metal coupons that were exposed for 56 days.
Final Report - February 2008                                                    Mexel Efficiency Study Page 9 of 24               DC Cook Nuclear Plant

Artificial Substrate Analysis (Plexiglass Baffle Plates)
Baffle plates were installed to prevent short-circuiting and to direct flow to the bottom of the biobox where the slide racks were located. Images of the baffles were taken for the record and can be seen as Figures 3 & 4. The results are shown in Figure 5.
Figure 4 - Control Baffle Plate AEP, Donald C. Cook Nuclear Plant H-O-H A-432 IMexel) Testing Post-Veliger Density & Average Post-Veliger Size from Blobox Baffles Control vs. Treated Population Reduction- 8206                                                                  -0%
1400-35,000                                    ________                           ..
301M                                Cor.trol (   14,257                                     Can"roi- (+/-) 3.232 Treated-     6/-)4S80 I                                   Treated- +-/-179r    -
20,000...                                                   .+
ISC-300 V 10.00
 >                                                                   200 0-10 Month Exposure 10Month Exposre EContra         Tr.t'd Figure 5 - Post Veliger Density, Treated vs. Control (Biobox Baffles)
Figure 5 illustrates Mexel treated and control baffle plates. Cumulative settlement population densities were reduced by 82% as compared to the control group. Average post-veliger size, also illustrated in Figure 5, indicates a reduction in size by a cumulative average of 80% as measured on baffle plates. The population and size reduction shown is based on two baffle plates that were exposed for ten months.
Final Report - February 2008                                                           Mexel Efficiency Study Page 10 of 24                   DC Cook Nuclear Plant

Total Mussel Debris Collection / Ouantification Flow to the test rig was established after installation on August 30, 2006. Other than periodic maintenance and inspection outages (2 hours max.) flow to the rig ran uninterrupted until project completion on August 29, 2007. Upon completion, the center section of each of the flow trains was sealed to capture mussels and debris. Each cell was carefully disassembled off-site and the debris was collected by Custom Technique #4 above. This sample represents the expected difference between treated and untreated intake tunnels.
Figure 11 - Final Mussel Debris Collection Amounts Final Report - February 2008                                         Mexel Efficiency Study Page 11 of 24              DC Cook Nuclear Plant

Total Mussel Debris Collection / Ouantiflcation. continued The mussel debris was collected and transported to H-O-H Chemicals to determine volume and weight collected from all three spool pieces. Table 2 illustrates that a total reduction of 98.7% in mussel shell debris by weight in treated vs. untreated and 95% reduction by volume. Figure 12 displays this data graphically.
Table 2 - Total Mussel Debris Collection / Quantification Data Table Sample             Weight Collected, (g)              Volume Collected, (mL)
Treated                                    1.72                            <10 Control                                  136.26                             200 Growth                                 1764.50                             4000 Treated vs. Control Weight Reduction, (%)                            98.7  %
Volume Reduction, (%)                            95.0  %
AEP, Donald C. Cook Nuclear Plant H-0-H A-432 (Mexel) Testlni Mussel Debris Harvest Quantification Growth, Control, & Treated 1600 L/
Iw 1400 I    Iwo a00 0    400-,"
ZO' I-Yea A~curulat~a I  *Grwtdh        *CadmUc     ~   Tteaftd    I Figure 12 - Mussel Debris Collections Quantification Graph Final Report- February 2008                                                        Mexel Efficiency Study Page 12 of 24                      DC Cook Nuclear Plant I-

Corrosi6n Rates'Usine Mexel Table. 3 sh6ws corrosion rates for -steel coupons exposed to Mexel treated water versus untreated. water. Mexel treated coupons had-a 23% reduction in corrosion. rates. This. is based on the Weight loss differential intreated vs. untreated. Corrosion rate evaluations in tmils per.
year (MPY) are established by H-O-H guidelines for. cooling water- systemns and are listed in Table,4.
Table 3 -. Corrosion Coupon.Results, Treated Coupons, Cumulative, Coupon No.      Material        Days        Treatment        Weight:       Corrosion      Evaluation Exposed                       Loss,(g)      Rate,.(MPY)
T-75K,         Steel           43          Mexel          0.2989           3:89            Fair T.-75J         Steel           43          MeXel          0.2947           3.84            Fair T-80P1         Steel           56          Mexel          0.4109           4.11            Fair&#xfd; T-80R.         Steel           56          Mexel          06.190           6.19            Poor T-83K'         Steel,         200          Mexel          04594            1:29           Good Average         Steel'         100          Mexel*         0*4166           3.86            Fair Control Coupons, Cumulative, Coupon No.:    Material        Days        Treatment        Weight        Corrosion      Evaluation Exposed:                      Loss, (g). gate, (MPY_)
T-'75I         Steel           43          None           0.6537           8.51       Unacceptable T-75L          Steel,          43          None           *0.6093          7.94       UnriAdeptable T-80Q          Steel           56          None           0.3446          -3.45          . Fair T-80S.         Steel'          56,         None           0.3594           3.59            Fair T-83L          Steel         200-          None           0.4761.          1.33           Good AVerage,        Steel.         100          None           0.4886    ]      5.00      1     Poor Table 4-- Corrosion': Coupon Evaluation Standards (MPY)
Evaluation                                Steel Excellent                             0.00 - 0.99.
Good                                   1.001- 2.99 Fair                                  3.00 - 4.99 Poor                                  5.00-6.99 Unacceptable                          -7.00 - Over Final Report-&#xfd; February 2008                                             Meixel Efficiency Study Page [3 of 24               DC Cook Nuclear Plant
                                                                                                       )G

Internal Investigation and Observations Video boroscope evaluations of treated, control, and growth flow trains (pipes) were performed at regular intervals during this study. Figures 13, 14, and 15 illustrate typical results of the internal investigation of the spool pieces.
Final Report - February 2008                                     Mexel Efficiency Study Page 14 of 24       DC Cook Nuclear Plant I 'o

Internal Investigation/Observations, continued Figures 13, 14 & 15 are still photographs captured from boroscopic video inspections near the completion of the trial. The video inspections indicate the test rig compares well with observations made during previous remote operated vehicle (ROV) inspections of the 16-foot diameter tunnels. The untreated control and growth sections of the test rig reveal similar fouling patterns. The mussel attachment is observed in the upper portions (9 o'clock to 3 o'clock) of the tunnels and flow trains while the bottom sections are cleaner. This is believed to be the result of the scouring effect suspended solids and debris have on the bottom sections of the tunnels as well as the control and growth sections of the test rig.
Also, the mussel attachment is found on the downstream side of the corrugations. Colonies up to several inches thick have been observed in the 16-foot diameter tunnels as they attach to the metal within the corrugation troughs. This pattern is clearly observed in both the control and growth section of the test rig. Figure 14 shows colonization within the corrugation troughs and Figure 15 illustrates clumps of zebra mussels thriving in the low velocity environment. The velocity in this section is believed to be parallel to the downstream side of the corrugation troughs and trough bottoms in the 16-foot diameter tunnels.
In the test rig the dimension of the trough limits the numbers of colonies in the control section. However, the attachment and infestation mechanics are the same. The growth section confirms similar behavior as the tunnel corrugation troughs illustrated by colonization and clumping.
Figure 13 is a snapshot of a Mexel treated section of the flow train. Infestation patterns are not found in this section. The trough bottoms are free of attached zebra mussels and only widely scattered individual mussels were observed in this section of the test rig.
Figures 16 and 17 show the bioboxes after completion of the study. The orifices shown are the biobox outlets. From a visual inspection, the difference in population density and size is apparent.
DSOtAAOewDClaG                                             ftFc Figure 16 - Treated Biobox                           Figure 17 - Control Biobox Final Report - February 2008                                           Mexel Efficiency Study Page 15 of 24              DC Cook Nuclear Plant I-]

Total Suspended Solids Suspended Solids in any water system typically contribute to flow restrictions, plugging and debris loading. In an effort to understand solids loading, each week water samples were collected from both the treated and untreated flow circuits of the test rig. Total suspended solids were measured on each sample as the residue on a 0.22 micron filter in accordance with Standard Methods. The results show a reduction in suspended solids in the treated samples. These results are shown graphically in Figure 18 below.
AEP, Donald C.Cook Nuclear H-O-H A-432 (Mexel) Testint Percent Reduction of Total Suspended Solids Treated vs. Control 0%
0                                            ,
Z7014
    ~30143 0%-~
I ~IIIRtIIIIRRI
                                   ~qk' ~-~-            -    '~b *~* *@.b ~                -
Thre Figure 18 - Total Suspended Solids Reduction Figure 19 shows the visual difference of a treated vs. control sample in water clarity photographically. This potentially impacted the cleanliness of the bioboxes and test rig. It seems safe to conclude, that for reasons undetermined from the sampling, Mexel causes the rate of settling of particulate matter in the lake water to pass through the bioboxes and the test rig. Mexel will likely have a similar affect on the particulate matter passing through CNP's cooling water distribution system.
Figure 19 - Water Clarity (Treated vs. Control)
Final Report - February 2008                                          Mexel Efficiency Study Page 16 of 24           DC Cook Nuclear Plant

Figure 20 is a typical picture of two filter baskets during a weekly inspection, one from the Mexel treated flow train and one from the control flow train. Based on visual observation, the filter baskets from the Mexel treated flow stream consistently showed fewer mussel trans-locators, less mud, and less silt in the treated filter baskets compared to the untreated filter                            .tCr,,,             C, baskets.
Figure 20 - Sloughage Filter Baskets Slouahaae Rate Figure 20 also illustrates that the Mexel treated circuit caught fewer mussel debris and trans-locators. No increase of debris was found when fouled pipe sections of the growth flow train were installed in place of clean pipe sections on the treated flow train. Under this line-up healthy attached colonies were exposed to normal treatment dosage.
Mexel Mortality Exneriment
................. iI ---- F ........
To quantify potential impact on sloughage, a zebra mussel mortality experiment was devised.
Two hundred live healthy zebra mussels collected from screen house trash baskets were collected and placed into the treated and control bioboxes. The treated biobox received the daily dosage of Mexel and the control received untreated lake water. Live vs. dead mussel counts were made each week for five weeks. Zebra mussels exposed to Mexel illustrated a mortality rate equal to those not exposed to Mexel in the control biobox. Table 5 illustrates the relative mortality rate over a 5 week period. Therefore a massive sloughage of zebra mussel debris would not be expected should a daily application of Mexel at the 4 ppm dosage be initiated on a pipeline infested with zebra mussels.
Table 5 - Zebra Mussel Mortality Study Live Treated Mussels        Live Control Mussels Start                             100                          100 I Week                            81                            76 2 Weeks                           80                            75 3 Weeks                           80                            75 4 Weeks                           78                            73 5 Weeks                           74                            70 Final Report - February 2008                                                      Mexel Efficiency Study Page 17 of 24              DC Cook Nuclear Plant

Reverse Osmosis Membranes (R/O)
Zebra mussel chemical control agents can severely damage R/O membranes within the CNP make-up plant. (Water & Power Technologies, Technical Analyses Report, 2003 Appendix
#9). As part of this study water from the Mexel treated flow stream was fed through a small R/O unit that contained the same membrane material that is in the CNP R/O system, Dow Filmtec Polyamide (PA) thin-film.
AEP, Donald C. Cook Nuclear HUO-" A432 (MaxerL   T     i..na Fouled RO Membrane Deposit Analysis other 4.3%
40.1%                                                                                 53.2%
UCuIcim         scaboeame           *iiagnelwm i Figure 21 - Fouled Membrane Analysis R/O membrane autopsy results are shown in Figure 21. The test rig model illustrated no contamination with Mexel. Calcium and magnesium carbonates were analyzed to be 95.7%
of the deposit material on the R/O membranes. Autopsy results from the membrane failure in 2003 were determined to be deposits of clay and biota. The membranes installed in the test rig make-up plant model are the same as those operated by CNP, Dow Filmtec Polyamide (PA) thin-film. PA membranes are a thin layer of aromatic polyamide extruded onto a polysulfone substrate. The PA membranes intentionally have a negative (anionic) surface charge and are commonly fouled by cations. Highly charged cationic surfactants and cleaning chemicals are typically not recommended for contact with PA membranes. The fouling that occurred during dosage of GE Spectrus CT1300, a cationic quaternary ammonium compound, is theorized to have conditioned colloidal particles to attach to the membranes or impacted the surface charge of the membrane itself to attract colloids. This mechanism increased the normal fouling rate by rapidly depositing particles on membrane surfaces reducing salt rejection while increasing differential pressure. (Water & Power Technologies, Technical Analyses Report, 2003 Appendix #9) Mexel is non-ionic and did not illustrate the same behavior as Spectrus CTI300 in this test. These results illustrate typical membrane fouling by the insoluble salts of unconditioned positively charged cations.
Final Report - February 2008                                           Mexel Efficiency Study Page 18 of 24               DC Cook Nuclear Plant d2o

Or2anic Loading Total organic carbon (TOC) and total organic nitrogen (TON) were monitored to determine what affects Mexel may have on organic loading, both as the effective biocide and byproducts. Increased concentrations of organics by more than a factor of 10 over the control condition can have adverse effects on microbial fouling and in this case may be indicative of excess residual Mexel concentrations.
TOC and TON results are found in Figures 22 and 23. Concentrations of TOC and TON were comparable in treated and control flow samples. As any TOCiTON concentration increases due to Mexel were less than a factor of 10 over the control condition, there were no adverse effects on organic loading. This suggests that Mexel does not significantly contribute to TOC or TON in bulk water solutions.
A". Donald C. Cook Nuclear Control vs. Trat 1
1
              '~:
                                                    .e      oe sampift Da" Figure 22 - Total Organic Carbon (TOC) Results from Lab Samples AEP, Donald C. Cook Nuclear Icu Organic N~menpl    "rON Control vs. Treated 3.0 Saml    OaOp                 ~.
                                    *ob4   Fo    wt.      TaedFlwTd
                                                                         ~~4~
Figure 23 - Total Organic Nitrogen (TON) Results from Lab Samples Final Report - February 2008                                              Mexel Efficiency Study Page 19 of 24               DC Cook Nuclear Plant

Whole Effluent Toxicity Testing Summary' Two AEP Cook Nuclear Plant cooling water samples were collected by HOH and AEP/CNP plant ermployees on November: 29-30, 2006 for whole effluent toxicity (WET) testing'by Great Lakes Environmental Center (GLEC).
The first sample, was a 24-hr. composite samnple collected using an automatic composite sampler. Effluent samples Were collected
                                                  'every hour beginning on the morning of Nov.
29th. Sample collection- continued for 24-hours with the last sample collected the next day, Nov. 30th, during the 30-minute, Mexel treatment period. The 24-hr. composite sample was collected: to&#xfd; represent the effect, if any, on aquatic life during a typical 24-hr.: Mexel treatment regime. Figure' 24 shows collection Of wet test samples at CNP.&#xfd; Figure 24- WET Test Collection The second sample' was a grab, sample collected on the morning of Nov.30th-during the 3*0-minute treatment period. This sample- was collected to represent the plant discharge.
conditions at a maximum Mexel concentration and the. effect, if any, on aquatic life. if the treatment were to be run continuously for .24 hours, which is' not the recommended dosage regime for thills product.
The two, samples and a sample of untreated- lake water- for dilution and laboratory control were packed on ice immediateiy after collection in c0oolers on the morning of November 30th and delivered that day to the GLEC laboratory in; Traverse City, MI. The toxicity testing wasi initiated following sample delivery to the laboratory. GLEC conducted a 48-hour Daphnia magna and a 96-hour fathead minnow acuteAtoxicitytest on each of the 24-hr. composite and 30, minute-grab samples following standardized USEPA testing protocols, The 24-hour composite -sample,was not acutely toxic to D.rmagna or f'athead minnows, (See Figure 2&#xfd;5). There* was 100 percent survival of both D. magna and -fathead minnows in this
,sample. The 48-hour D. magna ,LC50 (median lethal toxicant concentration) and EC56 (median effect. concentration). estimates were both greater than 100 percent in that sample.
The 96-hour fathead minnow LC50 was also greater than 1.00 percent in-that sampler The 301-minute sample was acutely toxic to both.D. magna and fathead minnows. The acute toxicity, tests that, were, initiated with the 30-minute sample had an estimated LC50 of 35.4 percent sample. in the D., iagnatest and 2737 perceit'sample-hin0 the fathead: minnow test. If we assume an estimated concentration of 2.5 ppm Mexel residual, in the sample, these. LC50 estimates equate to estimates of 0.88 mg/L Mexel for D. fiiagnq and 0.69 mgIL Mexel for fathead minnow respectively.
Final Report.- February 2008                                              Mexe Efficiency Study Page 20.of 24               DC Cook Nuclear Plant

Whole Effluent Toxicity Testing Summary, continued AEP, Donald C. Cook Nuclear H-OH A432 (Mexel) Testing Whole Effluent Toxicity (WET) Test Results "10 log%
90%
j30%
j20%
10%
0%
24 Hour CWW06ft (11MM- IVJOM61 I  CDwdsoIa4c0=)            0UHW~eWoW~L)
Figure 25 - WET Test Results The toxicity tests with the 24-hour composite sample demonstrated that the recommended treatment regime of 4 ppm dosage Mexel to obtain a 2.5 ppm residual concentration for 30 minutes per day caused no negative impacts to the aquatic life using the indicator organisms D. magna and fat head minnows. The toxicity tests with the 30-minute grab sample demonstrated that a hypothetical continuous treatment regime of 4 ppm dosage Mexel to obtain a 2.5 ppm residual concentration would exceed the threshold toxicity for these indicator organisms without a 3:1 dilution credit (0.83 ppm) for the plant's high velocity discharge diffusers.
Based on this data, it is logical to hypothesize that given a 3:1 dilution factor (GLEC Mixing Zone Evaluation 2005) the Mexel treated effluent sample would not be toxic to aquatic life within the discharge mixing zone in Lake Michigan. To confirm the hypothetical treatment regime described above, another (WET) test was conducted using the same indicator organisms with a Mexel treated sample. On August 28, 2007, a one gallon sample of Mexel treated cooling water was collected from the pilot test rig and mixed with two gallons of untreated lake water to simulate a 3:1 dilution. That sample and an untreated lake water sample were packed on ice in coolers on the same day and transported immediately to the GLEC laboratory in Traverse City. The toxicity testing was initiated following sample delivery to the laboratory.
The August 2007 diluted 3:1 Mexel treated effluent sample was acutely toxic to both D.
magna and fathead minnows. The 48-hour D. magna and 96-hour fathead minnow LCs0 (median lethal toxicant concentration) and D. magna 48-hour EC 5o (median effect concentration) estimates were all 65.9 percent effluent.
Final Report - February 2008                                                       Mexel Efficiency Study Page 21 of 24                 DC Cook Nuclear Plant

Whole EfflUent Toxicity Testina Summary, continued A direct comparison of LC5 o estimates between the two tests cannot be made. A, relative comparison of the. two sets of WET tests may .be possible by eqtrap0lating.the toxicity test; results to simulate a 3:1 dilution with. the November 2006 results or to simulate. no dilution using the August 2007 results. Using that comparisonr, the. results of the Auguist: 2007 Mexel treated cooling.water toxicity tests after a 3:1 lake water dilution are similar to the November 2006 toxicity test results in that the :results are Within the expected variability of whole effluent toxicity tests. In interlaboratory comparisons, EPA determined that: WET test results may vary by one test concentration between laboratories and over time; Likewise,         in single chemical toxicity tests with. Mexel against Dgphnia nagna and fathead minnow, a similar degree. of variability was observed. In: the Mexel toxicity database, the toxicity of Mexel to Daphnia magna' varied betweeif 0.120 mg/L and 0.595 mg/L. The 'toxicity of MeXel to fathead minnow varied between 0.360 mgL and 0.660 mg/iL. However, these comparisons should also take ifito consideration the differences in the physical and chemical attributes that may affect the toxicity of Mexel in: different water types or treatient' scenarios, because of" seasonal changes in water quality, or because of different.sources of test organisms.
Final Report- February 2008                                            Mexel Efficiency Study Page 22 of 24.             DC'CookNdclear Plant

Conclusions Zebra mussels were probably introduced into the Great Lakes in 1988W Since then many water intake facilities have been affected and have initiated a control strategy.
The continuous&sect; flow research facility has enabled an on-site evaluation of a zebra mussel control technique to test the, effect of Mexel on natural populations of veligers and trans-locators. Corrugated pipe sections, accurately 'simulated the plant's intake tunnels :as confirmed by the observation of sinilar fouling patterns. Robust data have beenocollected to predict the,impact Mexel will have, on plant systemfis; and the environment without the cost of a full scale. application. The insight provided by this evaluation enables a better understanding of the proposed.Mexel application while mitigating risk and failure.
    .1. Mexelf treated circuits illustrated an aggregate 78%. reduction in post-veliger population density anda,64% reduction in. post-veliger size compared to. untreated
        -circuits as evaluated by CNP procedure l2-EA-6090-ENVV-I!01 Zebra Mussel Sampling and Analysis.
: 2. Mexel' treated circuits 'illustrated no discernible pattern of infestation, colonization or clumping. Rather the mussels exhibited a pattern of isolated individuals compared with the controiwhere mussels formed, clumps and were abundant.
: 3. Mexel treated circuits realized a 95% reduction by volume in mussel- and total debris compared to untreated circuits as measured. by the total material removed fromthe flow circuit piping at the end of the pilot test experiment.
: 4. Mexe reducedsilt accumurlat ion in the treated biobox and test rig.componentS when compared itothe untreated'biobox and comporients.,
: 5. Mexel treated circuits did not illustrate a mortality- or sioughage rate greater than.
control circuits, thus im proving the understanding that normal product application, to0
        .a fduled'tunnel will not iresult in a.massive release of mussel debrisor oqerload .of the, traveling screens and debris' handling systemrs..,
: 6. R/O membranes, were not fouled with colloidal particles, conditioned :by Mexel or' Mexel molecules.
7    Whole:effluent toxicity testing illustrates speciessurvival 'ini 100%:of the effluent of a 24 hour composite indicating no impact to indicator organisms under- studied' treatment regimen,
: 8. Whole effluent toxicity tests using. Mexel treated' Water,'showed. a48 hr Lc 5 0 toxicity of 35,.4% to Daphniamagna and 27.7% to, fathead minnows for an undiluted acute sampling and 65.9% for both species for a3"1 diluted acute sarmpling.,
The results of this'study indicate, that Mexel is effective at preventing infestation and. can be safely applied at the prescribed dosage regimen without negative consequence at CNP or the biotain Lake Michigan.
Final Report- FebruarY 2008                                             Mexel Efficiency:Study IPage 23 of 24            DC'Cook Nuclear Plant

Acknowledoement.
H-O-H Water Technology would like to thank the following contributors:,
: 1. American Electric Power (AEP)
Eric Mallen,- Environimental Specialist John Carlson - Environment Safety & Health Manager
            > Jon Hamer-Environmental Manager Alan Gaulke - Consulting Environmental SpeCialist
            > AEP Cook Nuclear Environmental DepartmentStaff
: 2. RTKTechnologies.
            > Rick Kreuser
: 3. Ecotox. Inc.
Francois Ghillebaert - Aquatic Toxic0logist, PhD
: 4. Great Lakes Environmehtal
            > Dennis McCauley - Operations Co-Manager/Principal Research Scientist
: 5. Michigan Department of Environmental Quality (MDEO)
            > Sylvia Heaton%-Aquatic Biologist, Senior Level I
References
: 1. Ackerman, J.D., Claudi, R., Spencer, FS., Si, B, Evans D., .A Continuous Flow Research Facility for Zebra Mussel' Research & Control ptesented. at the 4At international zebra mussel confertence Maidison Wisicnsir1994.
: 2. Ghillebaert,,F. 1997Toxicological File of Mexel 432, Summary of the'Studies.
: 3. KreuSer, R., Vanlaer, A., DamoUr, A. 1997 A Novel Molluscicide. Corrosion Inhibitor and Dispersant, Corrosion 1997,, Paper No. 409.
   .4.. McCauley, D.J. , Endicott, D.,. 2005 Mixing Zone Evaluation for the DC Cook Nuclear Plant Discharge PlUme in Lake Michiguan.
: 5. Mexel Skid Baffle AnalySis 2-15-07, E..Scott Rose-AEP.
6.: Water & Power Techn1oogies, Job #7574,. Technical Analyses Report, JUly2003.
Final Report.- February 2008                                      Mexel Efficiency Study.
Page 24 of 24            DC Cook Nuclear Plant

APPENDIX 1 Project: .mipct S*ydy of lllexel on Zebra Mlussei Infesta.tion Continuousv-FiowY Resear1ch1 Facility Athorse- Jon J. Cohen, Tom Armon, Dave Jlinge, DariuS Barkauskas, Henr A.Becker Rikk Kreuser, Francois Ghiltebaet Su mmnry Mexel, a filming 'amine proposed for zebra musselcontrol, will be-studied using nativ, colonies and plant intake water at. the Cook Nuclear Facility,, A UiqUe continuous-f1Ow re&sect;earch- facility built to moodel Cook fP o and ope rati-Inal concditions is proposed for this prtqet. Data wll be deyeloped to test the effectiveness of Mexetunder realistic conditions using natural populations of larvae and translocators. It is belieVed that this acility will provide valuable insight' into the uie.of Mexe, as a preventive molluscicide as -well as tecl&#xfd;t-iq*es:for optimization arid ptotection of critic,91tplan't water systbms., -Control of ner zebra mussel infestation, injection of Mexel; affects ofMexeil on e xisting mussel infestation and Mexe deposition on-pipe will be examined.
-Continuous Flow Research Facilities have een used:toi'model effectiveness:of molluscicide control programs on once tIhrough, cooling water systems.
(Ackerman/Claudi 1994). Modular flowbthrough design using natraltpopulations have previously provided rob .ustdata that enabled treatment xnodeling for plant Systemrs under dynamic conditions.
I'kgst is a filming amine used in many freshwater and :saltwater systems dn Mexel worldwide. Todcologica effects of Mexet have been-widely studied in b6th freshwater.
and saltwater (Ghillebaert; 1997), While there is little published dataconcerning Mcxcis.
affectfon. existing zebf": fniissei infestations&sect;, depositinnocortugate4 pipe with exiscing infestation and effecfiveness in freshwateir applications. An.overvie-w-study of Mexel's, use as a molhusci ide, inhibitor and dispersait (Kreiuser etal, 1997), demontrated efficacy in fresh and salt water when proper film formation was acchrhpliShed.
Biodegradation of Mcxel -wasi also demnostiated and documented.
Specifically with respect to Codk Nuclear Facility, intake tunnel modeling, use with Lake Michigan water and infestation currently within their water intake tunnels must be studied before continuous application can be initiated. Application of Mexel at Cook must~be:accomplished without interkrptidn-of plant opetation including plant water intake and plant water pretreatment equipment.
Cook Nuc lear Facility has dealt with zebra mu;ssel infestation for seval years and has' employed many differeait treatment alteriunaiytis. Three intake tunnels construcedof16
&#xfd;foot diameter crn-ugated, galvanized steel pipe extend out one half mile: fiom a forebay.
Corugatlions in.this pipe,are six,inches wideaand two inches deep. Preventative and shoe-k
,zebraimussel chemistry has: been-discntinued for mul1tiple spawiing seasons,.allowing colony growth along.,all three intake tumnels; with acumulation contaned&#xfd; within the cormgatignS.
I

Cliheical, injec.tion p'ping, wihckh had-previopsly run.from inside the plant to injection assemblies at the tunnel entrances,. has been dam agecl and will be replaced prior to continuous Mexel.injection, Injection assembly design is critical-to Mexel,effectivenesg and results of this sitdy due to the fi lmiing natufe. of this prodiict.
Contct between Mexel and pipe surface must be ensured to. allow a uniform film along the intake tunnels. Film formation on &#xfd;clean and infested pipe: nibst be studied-with near in-situ flow and distribution characteristics to model effecjivehoess in the Cook iblant.
Redesigned traveling screens. in place at Cook haveproven to be more:,effective at
_handling debris and dislodged mussel colonies-priorlto, plant watei distribution to condensers and sfrvice water systems. HoWever, a stidden or massive release of&#xfd; colonies and shell debris in theory have the potential- to oyer load the travelinmg screens As-well, as compromise the plants abilitylto dispose of shell.debris. While it is desirable to.remo-ve existing colonies in part the goal :of this study i'srto. exfrapolate a rate of sloughage at
-recommended normal Mexel dosage. regine.
Experiment: A novel experimental apparatus is proposed for study of zebra musselsiat the Cook Nuclea Farcility 'This.apparatusis comprised of horizontal corrugated spool pieces acting as a substriate for zebra mussel 'growth&#xfd;. tranislocation andMexel deposition.
Spool.pieces will be mahufactured from.stainless. steel pipe and four feetiirhlength.- Pipe diameters: will be four inches: Two identical piping trains will provide a cpnirol'scenario in which Mexeli will no tbe introduced and one-where Mexe!'s effects will be studied.
The tr*in wI be provided .a service flow, of 750 -gpm.with a sikty ho'rse power pump. The flow train manifol diverts flow to service a total of three trt          and mihmaintan velocity consistent -with normal tunnel velocity. Thisflow rate will allow a velocity range of 6 to 7fPs in the treated and control pipes, while the growth flow pipe will runat"I .1 ..
Varying flo-w velocity will allow.forPchanges in.shearstresses mad contact time between.
'the flow stream -andsurface. Sihce Cook',S ithtake tunnels are 1.6 foot diameter:corrugated pipe with zebra mussel infestation, calculating boundqry layer depth, actual flow velocit&#xfd;y within the boundary layer and shear. forces at surfaces is difficult -andunreliable.
Through. studying various flow streams multiple scenarios *will be evalUated to ensui ethat any worst case circumstancecan be determined ekpetirmientally before introducing product into Cook's intake turinel.
Our experimenta apparatus is comprised of thrxee sections a treated, control and growth..
Make-up plant modeling is also included. The attached drawingdetails the entire experimental apparatus ,ith diagnos&c equi*mentesectins, pui'mps and plat pretteatment etilipment. A branch with a booster purmp will proyide wvater through pretreatient etuipment, for make-up plant modeling.

Experimental design allows several configurations of both horizontal trains providing a means, tO test all four main paramneters. Sppql piece sections are twelve feet long, consisting of three.four foot sections. Section lengths provide eniough axial lehgth.to normalize flow characteristics. Foreach length, the third spool piece will provide.'best data available for each flow train due to projected achievement of lanunar.
Precise flow measurement will provide, accurate llow/ velocities-in: spool piecO bections.
All other monitoring equipment for meaisbren-ients in the horiz ttal experimental section will be loeated in.a pipesections.ubsequent..to the final .spool section. A detailed list of monitoring-equipment is attached.
In addition to on-line instrumentation weekly grab samples will evaluate gen.eral.
chemnisty, Mexel concentration;. Total organic carbon (TOC);, total organic nitrogen (TON) and :turbidity will provide information on degradationAof organic material.,
Turbidity will be usefbl in determining macr.oparticulate, released from colonies and mussels after Mexel addition. TOC and TON will provide.data onibore finely released material, metabolic byproducts and a measurement of Mexel! in the water. Corrosion coiipons will measure potential differences:onr    metal sirfaces and are commonly used to determine corrosion rates.
Spool.pieces can be rearranged lto pkovide altete .exPerimentai configurations. Spool pieces with well developed grvowh will, be relocated to treated sections-to understand isoughage and cleanup rates.:
Plant :pretreatment eq~ipment isa criticaltcomponent to plant operation at: ookNuclear, Facility. Previous zebra mussel treatments. have caused: significant damage to reverse osmosi s menibranes:amongother equipmentdifficulties. Multimedia filtration, carbon-filtration,.particle filtration and reverse osmohsis will be, fed by a ten gallon per minute booster pump. Effects ofMexel On each piece of pretreatment equipment will be determinied to prevent imtpedimentsto operation of plant eciuipment and :plan-tishutdown.
Concllusi ns will: provide plantspecific information forcontinual use of Mexel at Cook Nuclear, Facility. Data collected will detail: how Mexel should be injected, effeets. on existing infestation in Cook's intake ttnn*l*s, prevefttion of ffitdtre gtowth and.effects on:
pretreatment equipment. Experimental data will also provide a roadmap to circumvent obstacles to proper plant operation.
: 1. Ghillebaert, F. 1997 Toxicblogical File of Mexell 432, Summary of the Studies
: 2. Kreuser, R., Vanlaer, A., Damour,. A. 1997 A Novel Molluscicide. Corrosion.
inhibitor and, Dispersant, Corrosi on' 1997, Paper.No. 409
: 3. Ackerman,a.J.D., Claudi, Ri., Spencer, FP.S., Sim,. B.,Evans'D., A Continuous Flow Research Facility for-Zebra:Mussel Research.& Contr.ol peserited -at the 4-'
international zebra riissei, cdnfereiice Madison. Wis.consin .994

APPENDIX 2 REVIEW AND APPROVAL TRACKING FORM Section 1- ProcedureIiiformati6n:.
Number: 12-EA-6090-ENV -.11                                                                                Rev. 3

==Title:==
Zebra Mussel Sampling and Analysis Section 2. -Alteration Category:-                            "
:lMinor Edit6rial`Coirection ..                                      Canceflation I Major Editorial* Correction -(Full Review)                                 Superseded by (1ist -I superseding procedures):
Mnor Re vision_____________________
mi El Major Re.'ision ,(Full:Review)                                    E- New     Procedure (Full Review),
Section 3.- Temporary Procedure/Revision:                                                         ........
SN/VA.       El TemporaryPrbcedure                   El Temporary Revision,             AR No.:.       _________
 .Expiration Date / Ending Activity:                N/A Seciion 4&#xfd;- AssociatedConfigurationImpact Assessments:.
Change Driver,/CD Trac.king No(s).                                                                      .                           N/A, Section 5-- Reviews:
7a   0 Department                                              U~3a tz                  O-           (U (Refer to :Figure 6,: Determination 6f Requtiired Reviews),
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- Section 6- Tech~nical Review:
Updated Revis'ioan'SummnIar~y andfmpemientation Plimi (ifapplicable) attached?                                0 Yes.            ...
Impneomentation Plan developed?                         If yes, AR No.:;'El                                         Yes:              N/A
.Are ihere implementation actionsIt be c6mipleted prior tO 'the effective date?                                 [I Yes            S     No, 10 CFR 50.59Requirements-complete?. Tracking;No.:_n_[-'E                                                            Yes         :09 N/A Technical Reviewer:                     . ,'.    -                  <"       '.                               Date:                __06
..Section 7',- Ready*fr A                       .    .rov:.                    .
Administrative Hold Status::             Eleleased..         j]Reissued       .0  N/A         CR No.: -_...               ............
Writer:                                                           .Date:
El Ops ManageI Concurrence:&#xfd;                    N/A                                                        Date:
Section 8 - Approvals:..
,P0.RC IReview -Required:                             ElY \)4           No                     Mt& No.
Approval Authority Review/Approyal:                                                                        Date:     _________"
Effective Date:
Section 9- Follow-uY Actions: .
Commitment Database'update requestedd n acrnce wlth pM-2350-CMS-0Ol?,]                                              Yes         0 N/A NDM notified of-new records or changes to records that could'affect record retention?'                     .        Yes               N/A
* Office Information.For Form.Tracking Only - Not Pant'ofForm
                    -"This                     form is denied from the inr-f, mtiion-rin PMP-2010-PRC002,.
Procedure Alteration, Review., and Approval; Rev. 19, Data Sheet 1, RReview and Approval Tracking Form, "Page                                               I of I

         *ES                      12-EA-6090-ENV-101                                           Rev. 3                     Page 1 of 10 Zebra Mussel Sampling and-Analysis Information Marcia Strefling                          John Carlson,                                               Environmental Writer                              Document Qxvner,                                        cognizant O*dgni.ation TABLE OF CONTENTS 1  PURPOSE AND SCOPE, ...................                            ............            .......                               ...... ,;2 2i.
2  PREREQUISITES....             .       ,...   .......           ,.......                 .......................................            2:
: 3. PRECAUTIONS AND LIMITATIONS ...........                                                    .....       .......                             2 4  DETAILS....        ......... .,.....                 ........                             .....                o..      .............. 3 4.1    Whole Water Veliger Sampling .3 4.2    Whole Water Veliger Analysis.                                   .............                                                     4 4.3    Artificial Substrate Sampling..,..........................                                                                          5 4.4    Artificial Substrate Analysis            - Micros*ope Slides.....................                                                   6 4.5    Artificial Substrate Analysis - PVC Substrates.. .....................                                                              7 4.6:   Impromptu Sampling & Evaluations..................................                                         ........       ..... 8 4.7    Reporting       ..............................................                                                                     9 5  CORRECTIVE MEASURES ........................................                                                                               9 6  FINAL CONDITIONS ........................                                      ..........                              ,.......       .9 7  R FEREN          S                                   ..............               ..............................                           9

Information               12-EA-6090-ENV-101               'Rev-' 3-                  Page 2 of 10 Zebra Mussel Sampling and Analysis 1       PURPOSE AND SCOPE 1.1     To establish the-proper methods formonit6ring Zebra Mussels in the Donald C.
Cook Nuclear Plant in support of NRC commitments, Zebra Mussel Monitoring-and Control, and the GenericLetter 89-13 Programs..       Wr. 7.2:.1b, 7.2.ad, 7:2.Jl) 2        PREREQUISITES 2A       Environmental develops a sampling schedule for work. Deviations'maybe made at the discretion of Environmental.
2.2      The following sampling and analytical tools are available for evaluating zebra mussel densities in the whole water and, settlement on substrates.,
          "       bio-nonitors
* plastic barrel
* pump
          "       test tube racks                  .       floW meter or bucket and timing
                                                           .device
          "       microscope. slides
* 1 literplastic collection jars
          "       plankton net withcod-end bucket                                   PVC pipe sampler
* stereo-microscopewith
                                                            .I ml Sedgewick-Rafter counting polariiing.filters                                                                cell
* magnetic stirrer                         pipette 2.3      Access the D. C. CookPlant Zebra Mussel Monitoring Program Worksheet ExcelTM spreadsheet from: the Environmental "S" drive ait ESRIZebra Mussel Data/Zebra(year). X!s: Thiks:is a non-QA record and is noftfiled in the, Nucle:r Document Management'.syystem.
3        PRECAUTIONS AND LIMITATIONS 3.1      Technicians are versed in standard practices for sampling and monitoring Zebra.
Mussel counts in whole water and artificial sbstrate samples. I1ef. 7.1.11 3.2      Use ground fault interrupters or ground faulted outlets when plugging in sample:
pumps; 3*.37    Use faillprotection when working over openings inhdecking :or grating:

Information                  12-EA-6090-ENV-101                Rev. 3              Page3' of 10 Zebra Mussel Samplling and Analysis' NOTE:             The use of the Personnel Tool and Material AccountabilityLog (PMAL) is not required for the artificial substrates attached to a weighted rope at the screenhouse intake forebay location as they are designed to be installed/removed at this location.
3.4     When:installing/rernoving sampling equipment at the screenhouse intakel forebay location(s), use the FME Task Plan established'for Environmental Sampling activities in the screenhouse. Sampling equipment adjustments and repairs should be made using tools outside of the FMEA whenever possible.
4:      DETAILS
: 4. L    Whole Water Veliger Sampling NOTE:             Whole-water sampling may be conducted in the plant's intake, forebay or.off of plant'sidestreams or bio-monitors to determine velliger density in the lake water being drawn into the circulating water system or service water systems.
Collect two replicates (approx. 2,000 liters or 528 'gal. each) within an 8-hr period on each sampling date (determined by the sampling schedule).,
In the event a flow'meter is not available, a 2,000 liter samfple-can be estimated using a bucket and timing device to determine, the, flow rate.
1 gal. = 3,785L 4A.1.       Direct a measured. flow into a plankton net that is suspended'in a partially filled plastic barrel to minimize organism abrasion.
4.1.2       Direct the flow from the barrel back to a-floor grating or floor drain-to minimize the flow of water onto the floor.
4.: 1. 3,   Stop flow to net w.hen approximately 2,000.L/528 gal. has been pumped thru net.
4.1.4       Gently wash down the plankton net nto the cod-end bucket,.
4.1.5"      Use filtered water to transfer the sample into a 1-liter plastic jar.
4,. 6       IF needed, THEN add filtered water to the jar to ensure that a full liter is collected.

Informationi       [     12-EA-6090-ENY-101                 Rev. 3             Page 4 of 10 Zebra Mussel Sampling'and Analysis 4.2     Whole Water Veliger Analysis CUIN;The sharp edges on the cov-er slip arecapable of injuring peronl             Handl
[CAUION:with .care,.
4.2.. Obtain and. stage the following pieces of analytical equipment.
                     *stereo-micoscope&#xfd; with polarizing filters
* Sedgepwick-Rafter cell (S-R-C) or equivalent With cover slip
* pipette capable of delivering. a 1 ml. :ample
* magnetic stirring plate&#xfd;with stir bar copy of "'Standard Protocols for Monitoring a4d Sampling
                    'Zebra Mussels'" by J. Ellen Marsden 4.2.2-   Stir sample slowly prior to withdrawing I ml. sub-sample for analysis; 4-.2.3   Load S-R-C with 1 ml. sub-sample and cover.
4.2.4    Read S-R-C.
NOT:          Use "Stahdard Protocols for Monitoring and Sampling Zebra Mussels" as a guide for identifying' viable Veligers. Do not include broken or, dead veligers in the count.,
4..5     Dbetermie wheter dilution of 21-liter sample is necessary. per the' Whole Water Density Dilutibn Table maiintained on the Whole Water Veliger and Artificial Substrate, Post-Veliger Density Counting Form of thel D. C. Cook Plant Zebra Mussel Monitoring Progrtam Worksheet.
4.2.6;   Dilute 1-liter sample as needed.

Information               12-EA-6090-ENV-101,                Rev. 3              Page 5 of 10 Zebra Mussel Sampling and Analysis NOTE:          Size measurements may be taken simultaneously with counts.
4.2.7     Count number of li've:veligers per I hil. sub-sample.
4.2.8     Record counts for WW Sample Sets #1 & #2 on the WWholeWater Veiiger and Artificial Substrate Post-Veliger Density Counting:
Form.
4.2.9     Measure the breadth (widest part) of up to 50 veligers (chosen "at random"), but not more than 10 from each sub-sample.
4.2.10    Record micrometer reading on theService.Water Systems Veliger Size Calculation, Sheet for each veliger measured.
4.2.11    Remove an.d .carefully clean slide and cover slip.
4.2.12    Repeat steps:4.12 2, 4.2.3, 4:2.4, and 4.2.7 u 4.2.11 Until 10 (or'ah appropriate number of) -separate sub-samples have 'been analyzed, 4.2,.13   Enter "Multiplier"used" from the Magnification Factors Table on the Whole Water Veliger andi Artificial Substrate Post-Veliger Density Counting Form to. auto-calcuilate sizes.
4.3    Artificial Substrate Sampling NOTE:          To determine postveliger settlement in the circulating water, essential service water, non-essential, service water, and miscellaneous sealing and cooling water systems, artificial substrates may be placed in designated ocation(s) in the circulating. water intake forebay, and'in bio-monitors placed on plant system side-streaims. Artificial substrates 'measurecumulative settlement over time. The sampling of the bio-monitors will be determinediby the sampling schedule.
4.3.1'    Artificial 'substrate samplers will consist of'slide holde'rscontaining microscope slides placed in side stream bio-monitors or, as' for the4 circulating, water intake forebay, specially designed cages attached to a rope and weighted by a suitable weight. These substrate samplers are modified test tube racks-or holders specifically designed to hold microscope slides.

Information               12-EA-6090-ENV-101                Rev. -3             Page 64ofo0
                             ,Zebra,Mussel Sampling. and Analysis 4.3.2       Artificial substrate samplers, used for the circulatingwater intake forebay study, may also consist.of a section of PVC pipe ctut in half length-wise and
                 'held .together by alhose clamp(s) and attached in a vertical orientation to.
rope or cable that is anchored via a concrete block or other suitable weight.
4.3,3      Obtain and stage the following pieces of equipment:
e    empty test tube/slide collection rack or equivalent 4.3.4      Remove the bio-box or cage cover.
NOTE:          Handle slides by the edges and transport them carefully so that no Zebra Mussels are inadvertently damaged,.6or removed.
4.3.5      Carefully remove the appropriate number of glass slides (typically 6-10) fromdinstalled slide rack&.
4.3.6      Place slides i.ncollection rack.
4.3.7      Replace cover on bio-box or cage.
4.3.8      Restore flow thru bio-box as riecessary.
4.4    Artificial Substrate Analysis    - Microscope Slides.
4.4.1      Obtain and stage the following' pieees of analytical equipment:
* stereo-microscope with polarizing filters.
                       "Standard Protocols for Monitoring and Sampling Zebra Mussels" by J. Ellen-Marsden single-edge razor blade or similar scraping device 4.4.2      Carefully remove a slide from the collection.:rack being careful to
                 &#xfd;touch only the edges.
4.4.3      Carefully scrape clean one side of the slide.
4.4.4      Place slide, on microscope stage clean side downu; 3&#xfd;

Information              12-EA,6090-ENV401                Rev. 3                Page.7 of 10I Zebra Mussel Sampling and Analysis 4.4.5     IF density is > -60 organisms per slide; THEN refer to the; Artificial Substrate Density Dilution Table maintained on the Whole: Water Veliger and Artificial Substrate Post.Veliger Density Counting Form for sub-sampling to determine the number of organisms per slide.
NOTE:          Size measurements may be taken simultaneously with counts.
4.r4,6    Count number of settled veligers/adults.
4.4.7     Record-counts on the Artificial Substrate Size and Density Calculation Sheet and sizes on theService Water Systems Veliger Size Calculation Sheet.
4.4.8     Measure the breadth (widest part) of up to 50 organisms (chosen.
                  "at random") but no more than 10 veligers/adults per slide.
4.4.9     Record micrometer reading on'the Service Water Systems Veliger Size Calculation Sheet for each organism, measured.
4.4.10    Repeat steps 4.4.2,thru 4.4.9 until 10 (or an appropriate number of) slides have been analyzed.
4.4.11    Enter "Multiplier used" from :the Magnification Factors Table on the Whole Water Veliger and Artificial Substrate Post-Veliger Density Counting Form to auto-calculatesizes.
4.5     Artificial Substrate Analysis - PVC Substrates 4.5.1     Pull PVC sampler from the intake forebay:
4.5i2     Remove the clamp(s) and openup the two halves.
4.5.3     Scrape a representative one square inch section and transfer it to a.
S-R-C.
4.5.4     IF there is too much settlement that al! mussels will not fit into the S-R-C, TH EN smaller portions of scraping may be; transferred to the S-R-C.

    'Iiformation          i   i2,EA-6090-E NV-01         7,     Rev. 3             Page 8`4 10 Zebra Musisel Sampling and Analysis NOTE:         Size-measuremenis may be taken.. simultaneously with counts..
,NOTE:         Number of zebra mussels:per square meter (density) is determined by the following formrulla and is~automaticlly calculated on. the PVC Substrate Section:of the Artificial Substrate Size and Density Calculation 'Sheet.
Density (Zebra Mussels/mr)        [(#Sample 1 + #Sample2)/2] x 10,000/6.4516 Where: 6.4516 cm2 = 1 inch' 4,5.5    Count the'number of zebra musselsand record on the PVC Substrate section of the Artificial Substrate Size and Density, Calculation Sheet.
4.5z.6   Measure breadth of 50 randomly chosen zebra mussels and record Mmicrometer readings on the PV SubstrateSsection of the Artificial'SubstrateSize and Density Calculation Sheet.
4,5.7    Repeat steps 4,5.3 thru 4.5.6 fora,second sample.
4.5.8    Enter "Multiplier used"7 from the Magnification &#xfd;Factors Table on.
the Whole Water Veliger and Artificial Substrate .Post-Veliger Density Counting Form to auto-calculate siz&s.
4.5.9    Clean and return PVC gamplerto0 intake forebay if sampling is to be continued for the coming year.
4.6     tIpromptu Sampling & EvAluationS 4.6.1    Oecasionally, Environmental may perform additional studies, which may include evaluations of biocides on Zebra Mussel settlement and mortality.
The sampling protocols and scheduleswi1i be developed as requIired by, the
                ,&#xfd;study.
4.6.2     This procedure does not preclude the detection:of other' bi-o fuling species.
IF other bio-foulers are detected that could' cause problems in-piping
* systems, THEN report; the results to the Environmental Zebra Mussel' Monitoring and Control Program owner.
tl t

Informatidn             12-EA-6n90-E       4-101          Rev. 3                 &9,f 10 Page Zebra Mussel Samp.ing and Analysis:
4.7     Reporting 4.7.1    Environmental technicians develop and maintain all field-sampling records pertaining to these activities and report,the results to. theEnvironmental Zebra Mussel Monitoring and- Control Program owner.
4.7.2. An annual report is prepared by Environmental,, which-details thexesults of the"Zebra Mussel Monitoring Program.
4.7.3    Environmental proVides a draft for comment and a final copy Qf thpe annual report to the Generic Letter 89-13 Program Manager. [Ref. 7.2.1c!
5       CORRECTIVE MEASURES 5,.1    None.
6       FINAL CONDITIONS 6.1     An annual report on the methods employed and results of the zebra mussel monitoring has been prepared and submitted to Environmental and. thed Genetic Letter 89-13 Program Manager. [Ref:.7.2.1c]
7       REFERENCES 7,1     use 

==References:==
7.1.I    Stardard-Protocols for Monitoring and Sampling Zebra Mussels, JI Ellen Marsden, April 1092.
7.12    Writing 

==References:==

T72. I   Source 

==References:==
: a. NRC JE Bulletin No. 8i-03
: b. NRC Generic Letter 89413
: c. CR-991 1280
: d. NRC Commitments- ! 199; NRC Generic Letter 89-13, Service Water system problem &#xfd;response.

Information     [    12-EA-6090-ENV-101           Rev. 3            Page 10 of 10 Zebra Mussel Sampling and Ahaysis
: e. NRC Commitments 1223; NRkC Generic Letter 891:3,.. Service Water system problem .response.
: f. PMP-2220-001-001,.Foreign Material Exclusion (FME) 7.2.2   General References
: a. PMP-2010-PRC-001, Procedure Writing
: b. PMP-2010-PRC-002, Procedure Alteration- Review, and Approvals

REVISION 

==SUMMARY==

Number:      *12-EA-6090-ENV-101                                                    Rev. 3

==Title:==
Zebra Mussel Samling and Analysis Alteration                                          Justification As a result of aprocedure periodic review,           Periodlic review of procedure.
(00800020-02)', the changes listed below were made. The changes involved removing the reference to using a concrete block to weight the artificial substrate sampler,, and using the FME Task Plan established for the Environmental sampling: activities for FME.
concerns.
10 CFR 50.59Applicability                           This procedure qualifies, as a "Maintenance Activity" as described in: Section 4.2.2 of the 10 CFR 50.59 Resource Manual. It is a procedure for -"implementing surveillances and inspections", thus it is not subject to review under 10 CFR 50.59. There are no manipulations to SSCs in this procedure.
Step 2.2 - "Concrete blocks" Were revised            The use of a concrete block as a-weight -for out of the list of sampling and analytical           the rope that holds-the PVC. artificial tools available for evaluating zebra mussel          substrate sampler in the intake fotebay has densities.                                           been discontinued. The block disintegrates over time and can become an FME issue.           It stainless steel has since been replaced by a weight. (CR-m5259060) Change                          I NOTE Before Step 3.4 -'Mention of a                  A concrete block that was formerly. used asa concrete block being used as aweight was             weight for the rope has been replaced by a
..revised out of the NOTE&                             more robust stainless steel weight. Change.
Step 3.4 - Mention of '"FME Area,                    The FME Task Plan directs What, FME Area Standard" and "PMAL sheet" were removed              Standard applies and if a PMAL sheet is from the step.                                       needed per PMP-2220-001-001, Foreign Material Exclusibn. (FME) Change Reference 7.2.2b for PMP-2010-PRC-002                Updated reference with correct procedure title changed from '"Procedure. Correction,          title. Editorial Correction Criteria n'.
Change and Review" to "Procedure Alteration, Review, and Approvals".
Office Information ForForm Tracking.Only           -Not,    Partof Form           .
ThisiiSa free-f6rm as called obt in PMP-2010-PRC-002, Prc6edure AWlteation, Review, and .Appioval.                                                                             Page.._ of._._  j

APPENDIX 3 GL!E'C December 15, 2006 Mr. Eric Mallen.
                       -American Elecqtii power
                       .Donald C. Cook Nuclear Plant GOreat4 Lakes                 One Cook Place E,inVronmenltal       Bridgman, M1 49106 Center RE:     ACUTE TOXICITYTEST REPORTS FOR SAMPLES' APplijed.                     COLLECTED FROMAMERICAN ELECTRIC POWER, COOK Environmental                 NUCLEAR PLANT ONNOVEMBER 29/30, 2006 Sciences
  &#xfd;Ywmgl~onhfinax:ohl Dear Mr. Mallen; Great'Lakes Environmental Center (GLEC) has completed our analyses of the 48-hour Daphnia jraverseiIty         magna and 96-hour fathead-minnow acute toxicity tests performed ontwo different samples 739 Hastings St. collected by American Electric Power (AEP) personnel ofi November 29/30, 2006. The two Traverse City        samples analyzed were; a 24-hour composite sample that included a 30-minute Mexel dbse:at 4 MI 49686             mg/L (GLC Number: 7010) and a 30-minute samblecollect.ed in conjutnction with a 30-minute dose at 4 mg/L? ofMexel (GLC Number: '7009)t Lake Michigan water-collectediby AEp
. 231 941-2230 231"9412 2240 fax    personnel-was used as the ,diluti6n water, for theD. mnagna and fathead minnoW tests_.
The 24-hour cormposite:samplewaS- tacutely toxic to D.. magnaorfathead minnows. Ther was 100 percent survival of bothD., magna and fathead minnow in this sample. The 48-hour D.             -
1295 king"Ave    -,
Columibus            magna.LCo, 5 (median lethal toxicant concentratmin) and EC5 o(median effect concentration)-.
    -OHA 43212            estimates were,both greaterlthan-100 percent sample. The 96-hour fathead minnowLC was, 5
also greater than 100 percent sample;."
614 487-1040 61-4 &#xfd;8 7'12 920 la&#xfd;   The 30-minute sample, which hadcan estimated residual Mexel concentrationi-of 2&#xfd;5 mrg/, was acutelyjtoxic to both D. inagna and fathead'minnows. The acute toxibcity tests that were initiated withfthe *0-minute sample had an estimated LC* 0 of35-4,percentsample in tie.m.        inaga test anid
                      ,27.7 percent samlple in the fathead minnow test, ,Ifwe assume an estimated e&oncentrat on of 2.5 rmg Mexel/L inrthat sanple,.these LI and 0.69 ig MexelI/L, respctively.
J estimates equate to LC:estimatesofQ88mgMexel/L                     I Asa comparison, in 2004- L-EC ,e-asured a D. inagna LCso of 0.20 mg Mexel/L and a fathead mimnowLC 50 of 0:45 mgMexel/Linlaboratory toxicity tests. The differen'edbet&Weenthe curfent and 2004 LC 0 estimates may-befexplained by-the difference in dilution Wateriused for theftests and that we have no way'of knowing the true concentration of Mexel in these samples.. -However, we do know. from the toxicity, database for Mexel that the LC50 for D. mdg-na ranges between 0.120 mg/L and-0.595 mg/L, andbetween 0.360 mg/L and 0.66 mg/L for the fathead minnoWs.
Consequently, the differences observed between the laboratory measured 1iC 50 estimates and,the
_fieldbased measurements reported-here are not that great or unreasonable.
composite toxicity test data demonstrate that at this.level of treatment, a resulting I
iposite sample used in, whole effluent toxicity testing f6llowing a Mexe! tteatment "toxic.
                                                                                                                         ?
ffi&sect;LJ

Mr. Eric Mallen AEP-Cook Nuclear Plant                            2   -                        December 15, 2006 I
A summary of the test conditions forthe toxicity tests are included in Tables I through 4; Thel 48-hour and 96-hour LC50 and EC50 estimates andto~xicity test result* are imcluded in Report, Forms 1 thfough 4. The raw data are included in Appendik A.
if you have any questinso1r comnments concerning theresults of these toxicity tests ,please contact either me or Dennis McCauleyat (23.1) 941-2230. Thank you"for the.opportunity to provide this servi.ce.to American Electric Power-I :DonaldC. Coo0k Nucleirr Plant.
Sincerely,,
Mailee W. GOaton                                          Dennis J. McCauley Laboratory Coordinator                                    Principal Research :Scientist/
Senior Operations-Manager MWG:mng Eclosures
-1
                                                                                                    .1% . .
AI

TABLE I-FATHEAD MINNOW TOXICITY, TEST CONDITIONS 24 Hour Composite Lake Michigan sample dosed with 4 mg/L Mexel in one30-minute Interval Summary of Toxicity Test Conditions Pirnepbales proinelas, (Fathead minnow)
L. Test Species and;Age:                             Pimieplhaespromnelas, (Fathiead :minnowj 6 days-November25,2006
: 2. Test:Type and Dtirationi                          96-hour Static, with renewal at48.hours
: 3. Test Dates:                                       December 01,-05, 2006
,4. Test Tempetature:(&deg;C):.                           25 +1
'5. Light Quality:                                    Ambient Laboratory, 10-20 [tE/m:/s

===6.1 Photoperiod===
16 h light, 8.h darkhess
: 7. Feeding.Regime:                                   None.
.8. Size ,of Test Vessel:                             250 mL glass beaker
: 9. Volume and Depth of Test.Solutinfis:              200 mL,65 mm Vo.No. of Test Organisms per Test Vessel:                10 11*.No. of Test.Vessels.pef Test Solution:              2
: 12. Total No. of Test Organisms per'Test Solutibn:      20
: 13. test Concentrations (percent):                      100, 50, 25, 1,5,; and 6.25
: 14. Renewalof Test Solutions:                          48-hour-renewal
: 15. Dilution and Primar Control Water                  Lake Michigan GLC# 7008;
: 16. Secondary Control Water:                            Synthetic Laboratory (ModeratelyHard) 17.
Aeration:.                                        N one 118.. Endpoints Measured-Mortality (LC50)

REPORTING FORM i FATHEAD MINNOW ACUTE TOXICITY TEST 24 Hour Composite Lake Michigan sample dosed with 4 mg/L Mexel'in one 30-minute Interval Facility Name: .AEP Cook- Nuclear Plant                                                                                     NPDES Permit No.-:
Receiving Water: Lake Michigan                                Outfall:                                                      RWC: N/A Test-Dates: 12/061/06 - 12/05/06                              Test Species: Fathead minnows                                 Age Range: 6 daysold, Test Laboratoryv Great Lakes Environmental Center (GLEC)                                                                    Repoil Date: December 15, 2006 SBULK SAMPLE INFORMATION
         .SAMPLE                  DATE              ARRIVAL                   DATEOF            ARRIVAL. DECHLORIN            ARRIVAL,      ARRIVAL             ARRIVAL COLLECTION             RECEIVED          TEMPERATURE                  FIRST USE,           TRC          ATION?                 PH,            DO          AMMONIA DATES
: 1. 11/29730/06-             11/30/06             0.9&deg;C                    12/01/06:           NM              No                 8.07            12.0           NM
 -What test methods*.were used: EPA/600/4-90/027 and EPA-821,-R-02-012.
Describe any deviations from test methods:                  None Source.of testorganisms:; In house, lot #11/25/06 TEST. DESCRIPTION.
Fed/Un-fed: Fed                                         Food/Feeding Frequency:. Artemla naupli. 2 hours prior to 48-hourrenewal No. Replicatesper                                       No.-Organisms per Concentration:22                                        Replicate: 10                                 Effluent Filtered? No EffluentSample Typpe: SampleA.: Composite                                                            -Sample 2::
Dilueant-(o*): Lake Michigan Water (GLC#.7008)                                                       SecdndarY Cotntro0!(02): Syhthetic Laboratory Water (Moderately Hard) MH# 1479

==SUMMARY==
OF:RESULTS4 48-hour LC,,:>00P%
96-hour LC o >100%
Percent Mortalityper Concentration.
(Percent Effected per Concentration).
                                    '-Controls-             _-Effluent                                             Coincentratiohis&#xfd;-                        -....
Day 010               ,0,       6.25 Piercent         .
                                                                                       -i. Percent     25 Percent.          so Percent      100. Percent           Perceht 1                o.o)                  I0(b)               ,),0)                0(0)           0(0)         .      0(0)             0(0o
           ..2               0()                a(0)                   0(0),               0'(0)-          0(0)                0(0)'            0(0) 3                00(0)               a                         O(0)CO)                          00).
0(a)11                   1            O        _O) 4                                    b(o)
M                   ;00(o                0&#xfd;(o).o         0(0oi               0.(6)!           6(6)
  *Raw data sheets are included in Appendix A.,
: 0. Synthetic "Labr-toryWater (Moderately Hard)
,Inv*,s        tor: Mailee W. G rton                               Contact, Dennis McCauley                                  P hone No.: (231 1941-2230,
          -s-X 1-- -                                       Principal Research ScientIst/Manaqer of Oberations,
                                                                   ...                                           .                         Jr      Jr Signature          -                                             Title                                                           Date
                                 ,1

TABLE 2 DAPHNIA MAGNA TOxICiTY TEST CONDiTION.S 24 Hour Composite, Lake Michigan sample dosed with.4 mg/L Mexel In one 30-minute. Interval Summary of Toxicity Test Conditions&#xfd;.
Test Species and Age:                                  Daphniathagna, <24 hours old,
: 2. Test Type anid Dutation:                               Static,:481hours Test Dates:                                             December,01-03, 2006
: 4. Test Temperature (*CC):                                 25 + 1 5!,   Light!Quality:                                         Ambient Laboratory; 10.420 glEim-/s.

===6.1 Photop'eriod===
16 h'light,8 hdarkness
'7. Feeding Regime:                                        None
: 8. Size of Test Vessel:                                    30 mL plasticdcup
: 9. VolumeandiDepth of Test Solutions:                      15 mL, 20,mm
: 10. N6o, of Test Organisms per Test Vessel:
No* of Test Vessels per Test Solution:                 .4
 '12:. Total'No. of Test Organisms perTest    Sotlution:      20
: 13. Test Cncentrations (percent):                          160, 50, 25, i12.5, and 6.25
: 14. Renewal of Test Solutions:                             None:
Dilution and Primary Control Water:,                   Lak.e Michigan GLC# 7008 16'. Secondary Control Water:                               Synthetic Laboratory (Moderately Hard)
: 17. Aeration:                                              None Endpoints MeasUired:                                   Mortality (LC,) and Effedt`(EC,)

REPORTING FORM 2 DAPHNIAMAGNA ACUTE TOXICITY TEST 24 Hour CompositeLake Michigan sample dosed with 4 mgIL Mexellin one 30-minute InterVal FacilityName':AEP Cook Nuclear Plant-                                                                                   NPDES Permit No.:_            _
Receiving Water: Lake Michio,an.                               .Outfall:                                                RWC: NWA Test Dates: 12/01/06*- 12/03/06                                 Test Spedies:'.Daphnia maqna                            Age Range: <24 hours:
Test Laborat6rym` GireatLakes Environmental Center (GLEC)                                                               Report Date: December 15, 2006 BULK SAMPLE INFORMATION'
         &#xfd;SAMPLE                   DATE              ARRIVAL               DATE OF          ARRIVAL      DECHLORIN          ARRIVAL     ARRIVAL         ARRIVAL COLLECTION             RECEIVED           TEMPERATURE              FIRST USE:           TRC           ATION?             pH           DO        AMMONIA DATES.
: 1. 11/29-30106               11/30/06              0.9&deg;C*               12/01/06            NM             &#xfd;No,            '8.07         12.0         NM
'ND: Not Detected What test methods.weie used:- EPAI600/4-90/027 and EPA-821-R-02-012 Describe any deviations"fiom test methods:                     None Sourcerof test organisms: In House: BD 11-20-06 TEST DESCRIPTION
  'Fed/Un-fed: Un-'Fed                                         Food/Feeding Freqeficy:N_*e NO1Replicatesper                                            No. Organisms per Condcentiration: 4i                                         Replicate:,5,                           Effluent Filtered? No Effluen.t SramplebType: Sample 1- Composite                                                         Sample:2:
DiIuerit (0,)i Lake Midhiaan Water (GLC# 7008)                                                      Secondary Control (0'): Synthetic Laboratory. Water (Moderately'Hard) MH#'1479

==SUMMARY==
OF RESULTS                                                    48-hour LCo: >100 48-h'our ECs;:'> 100 Percent Mortality per Concentration
                                         .- ,-                     (Percent Effected per Concentration) a                                                                             .Cntrols.-
                                                                                                     -Effluent Concentrations-2
: 2. _o;(o)
((0 0,
(o) 0_,
                                               .0(o0oo)0:0 j    6.25 Percent 00(
12.5 o
Percent.
I 25 Percent 0'(0) 0o)-
                                                                                                                        '5sPercent o"(0) o (000
                                                                                                                                       %100 Percent:
0(0) 00L-Percent "Rawdata sheietsqei a icluded
                          '       in Appendix Al 02: Synthetic.L'aboratory Wate'r (Moderatey Hard)'
velq      tonr MaieeW. Garton

==Contact:==
DerinislMcCauley                               PIhone No.:. (231) 941-2230, 8            .
Principal Research Scientist/Manaa-er.
of Oberations:
                                                                                                               .                     I    I Signatu-e:                                                        Title
                                       /                                                                                      Date 5-I.

TABLE 3 FATHEADMINNOW TOXICITY TEST CONDITIONS 30 Minute Lakei Michigan sample dosed at 4 mg!L Mexel (2.5 mglL residual concentration)
Sunmmary of Toxicity Test Conditions; Pimephalespromelas, (Fathead minnow)
I. Test Species and Age:                                Pime&#xfd;phijlesip&#xfd;0melas, (Fathead'minno~w) 6 days-November 25; 2006
: 2. Tegt Type and Duration:                             96-hour Static, with renewal at 48 hours
: 3. Test Dates:                                          December 0105,' 2006
: 4. Test Temperature.( 0 C)::                            25 +/- I
: 5. Light Quality:                                       Ambient-Laboratory, 10-210 i+/-E&m-/s 6.. Ph6toperiod:                                          16 h light, 8 hdarkness
: 7. Feeding Regime:
None 8:. Size of Test Vessel:                                 2"50 mL glass beaker
: 9. Volume and Depth of Test Solutions:                  200 mL, 65 mm
: 10. No: of Test Organisms per Test Vessel:                 40 1I. No. of Test Vesselsper Test Solution:                2
: 12. Total No.-ofTest Orgai'sms per Test-Soluiion:
13, Test Concentrations (mg/L)::                          1.00; 50, 25, 12.5, and 6.25
: 14. Renewal of Test Sojutions:                           48-hours renewal:
15..&#xfd; Dilution and PrimaryControl'Water:                 Lake Michigan GLC# 7008
.16. Secondary.Control Water:                              Synthetic Laboratory (ModeratelyHard) 17.. Aeration:                                           'None
: 18. Endpoints Measured:                                    Mortalit (LCk,)

REPORTING FORM 3 FATHEAD MINNOWACUTE TOXICITY TEST 30 Minute Lake Michigan sample dosed at 4 mgIL Mexel (2.5 mgIL residual concentreatin)
Facility Name: AEP Cook Nuclear Plant                                                                                     NPDES Permit No,:
ReceiVing Water: Lake Michigan                                 Ouffall:                                                   RWC: N/A Test: Dates: 12/101/06 -12/05/06                                Test Species: Fathead minnows,                             Age Range: 6davs old Test Laboratory: Great Lakes Environmental Center (GLEC)                                                                   ReportDate: December 15; 2006 BULKSAMPLE INFORMATION SAMPLE                  DATE                ARRIVAL                DATE OF           ARRIVAL     DECHLORIN           ARRIVAL      ARRIVAL      ARRIVAL COLLECTION            RECEIVED           TEMPERATURE                FIRST USE            TRC"          ATION?               pH         DO        AMMONIA.
DATES7 1;. 11130(06               1v130106               1.0&deg;C.               12/01106             NM'            No                i8.12        12.2         NM What'tesf methods were used: EPA/600/4-901027 and EPA-821-R-02-01 2 Describe any deviations from test methods:                     None Source of test organisms:.. In house lot # 11/25/06 TEST DESCRIPTION Fed/Un-,fed: Fed.                                         Food/Feeding Frequency: Artemia naup/iii 2 hoursoprior to 48-hour renewal.
No. Replicates per                                         No. Organisms per Concentration.?,                                           Replicate: 10                              Effluenit Filtered? No Effluent Sample Type: Sample 1:,Composite.                                                            Sample,2,:
Diluent (0,): Lake Michi-gan Water (GLC#7008)                                                         Secondary Control (02): Synthetic LaboratoryWater (Moderately Hard) MH# 1479

==SUMMARY==
OF RESULTS*
48-hour LCso: 28.72%.
96-hour LCo:6 740 LC5 95%*o.LoWer Confidence: 23193%
LC-o0 95% Upper Confidence. 32,16 %
Pedrcent Mortality per Concentration (Percent Effected perConcentiration)
                                  -DControls-                                                          "-Effluentconcentratidns--
Day 0,        .         '0'     :6.25 Percent        12.s Percent      2s Percent          so Percent    100 Percent        Percent 1               *0(0):                o (b)               0:.o                0(0)o            0(0)            10*0 (1,00      100( 100):o.
o Z               0(60)                 0(0)         .      o'()                6.(0)          30(35)            100 (100*Yo     :
io  oboi_:_                ,,
3'               0'(0)                0(0)             -0(0)                  0(0)'          35(35).           100(100)        100:(100):             .I_
: 4.              0(0)                  0(00)                                   0 o()*           35(50)..
o           10 160o (0) fib&       i   (ioo)
"Raw~data-sheefs aie included in Alpperol! A,.
0p: Synthetic Laboratory Water (Moderately Hard)
Ivestiqator Mailee W. Garton-

==Contact:==
Dennis McCauley                               P1hone No.:. (231) 941-2230 Principal Research ScientistJManaae'r of OnDer~tions Signatultei                                                 Signat.)     Re..s ..                     " f 0.. "-t "_n ate........
T itle                                                        Date.:

TABLE 4 DAPHNIA MAGNA TOXICITY TEST CONDITIONS 30 Minute Lake Michigan sample dosed at 4 .mg/L Mexel (2.5 mgIL residual concentration)
Summary of Toxicity Test Conditions Test Species and Age:                                   Daph!nianiagna,-   <24'hours old 2."    Test Type aiid Duration:                                Static, 48 'hours
: 3. Test Dates:                                             December 01-03, 2006
: 4. Test Temperature (&deg;C)::                                 25-+1
-5. Light Quality:                                          Ambient Laboratoryj 10-20 .iE/mVs.
: 6. Pliotoperiod:                                           16 h light,* 8 kdarkness
: 7. FeedingRegime:                                          None Size of Test Vessel:                                    30 mL plastic cup Volume and Depth of Test Solutions:
15 mL, 20rmm, j10.
No. of Test Organisms per Test Vessel:                  5 No.:of Test Vessels per Tesf Solution:                  4
 .1.3, 12.
Total No.. of Test Organpisms per Test Solution:       20 Tesst Concentrations (mg/L):                           100j,50&#xa2;25, 12.5,:and 6.25 14.
Renewal ofTest.Solutions:                              None
: 15. Dilution and Primary Control Water:                      Lake Michigan GLC#'7008
: 16. Secondary ControlrWater:                                 Synthetic Laboratory (ModeratelyHard)
: 17. Aeration:                                                None
: 18. Endpoints Measured:                                       MOrtality (LC50) and. Effhct (EC50)
__________________________________________________________  +/- __________________________________________________________
SLt

REPORTING FORM 4 DAPHNIA MAGNA ACUTE TOXICITY TEST 30 Minute Lake Michigan sample dosed at 4 mg!L Mexel (2.5 mgIL residual concentration)
Facility Name: AEP Cook Nuclear Plant                                                                                  NPDES Permit No.:
Receiving Water: Lake.Michigan                               Outfall:                                                  RWC: N/A Test Dates: 12/01/06 - 12/03/06                              Test Species: Daphniamagqna                               Age Range: <24 hours Test Laboratory:. Great Lakes Environmental Center-(GLEC)                                                             ,ReportDate: December 15, 2006 BULKSAMPLE INFORMATION
         -SAMPLE                  DATE              ARRIVAL               DATEzOF           ARRIVAL    DECHLORIN           ARRIVAL        ARRIVAL     ARRIVAL COLLECTION              RECEIVED           TEMPERATURE            FIRST USE            TRC2        ATION?!               pH           DO       AMMONIA DATES
: 1. 11/29-30/06'             11i/30/06             09&deg;Cl                112/01/06           NM            No               8307           12.0         NM ND: Not Detected What test methods'were used: EPA/600/4-90/027 and EPA-821-R-02-012 Describe'any deviations. from test mhethods'               None Source of test organisms: In House: BD 11-20-06 TEST DESCRIPTION1 Fed/Un-fed: Un; Fed                                     Food/Feeding Frequency: None:
No. Replicates per                                      No. Orgahnsms per, Conceritration: 4                                        Replicate::5                             EffluentFiltered? No Effluent Saimple Type: Sample 1: Composite,                                                       Samplee 2:
DilUent (0*):    Lake Mihilaan Water (GLC# 7008)                                                ,SecondaryControl (04): Synthetic LaboratoryWater (Moderately Hard) MH# 1479

==SUMMARY==
OF! RESULTS' 48'hour LC,: 35;36 %
48-hour EC5: 35.36 %
LCP5 95% Lower Confidence: Not reliable LC         /95%
UpperConfidence: Not reliable PercentMortallty per Concentration
* _          _            _-                (Percent Effected per Cohcentration).
                                    --Controls"-.                                                   -Effluent Concentrationse-.
0
                                     .0i   ...         0i       6.25 Percent        125 Percent     25 Percent:         so Percent      10o Percent       Percentf 0(0)                 o0(0)            o(0)               o0(0)          0o0)             '2o f2O)       i6100(100 2                 0o                                    0o(o)o              0o            .00o100                lao;        100100o
 'Raw*data shfeets ai'e included in Ap'pendix A.
0,: Synthetic Laboratory Water (Moderately Hard)
Qlnvestigator: Mailee W. Garton                              Coritact: Dennis McCauley                                 P hone No.: (231)94,1-2230 P~rincinai Resear'ch Scienti~t/Man~ner AfOn~ntionnR' T Rtleac SignatureTitle:                                                     Date~t~ngeo'neain Signature:,                                                                                                                 Date

AppendixA Raw Data Sheets Ehlfioni~ehtal Center 5(e~

K)
CHAIN OF CUSTODY RECORD (TO BE COMPLETED ONSITE AND SUBMITTED WITH SAMPLES) 739 Hastings Street Traverse City, MI49686 Phaone: (2.31)i941-2230X Fax: (231) 941-2240
     ,Facility:       0O 60 -. QU*C*eAr                r-, "                                            Collector:
Location::..                     "oCjzse_                                                              Date:   t~~3
                                                                                                                       ~N/~('(vk~J  ~ a      c260c, Contact Person:          !r,-       ,    .1                                                         Witness:v    O'-     .A ls      -
Phone&'  Numer:               'LkoES-   ScA1o  '\-ky   OL0                                             Date:.-          LeC 3)   9~
                                                                                                                                                    .I
         *For   24-Hour Composite samples, please:
TRANSFER OF SAMPLES:
(Firstsignature is sampler, last.sigrnature .is authorized, laboratory represeiOntative,.)
SHIPPER. -                         RECEIVER                       DATE.                      TIME            TEMPERATURE 2.
_,J, Condition of-Sample Upon Receipt:                                                                                              X. Received on Ice:
ok it I~fcx~

GLEC                                         EFFLUENT AND RECEIVING: WATER
                                                                        *CHECK-IN FORM Grdat  -kes Env'lronmental Cenier CLIENT:       C C3.           -k )-I  C_0CQo, .4.                                          PROJECT NUMBER:                  I,         ) 0 (-D INVESTIGATORS:
INITIAL WATER CHEMISTRY (UPON RECEIPT)
L.&\,*, *Mo,                 .. *:,         t, , 2q.k&&#xfd;LA*,
DATE.RECEIVED:                                      INITIALS                M                'IS                                     ,w
____  __I"        _             i________        qj__                  0&'-      1-  wl *o GLC NUMBER:            "%.___                                                "o-                            "i0O*1             "-)-O0 COLLECTION DATE: (Time Interval)                                            i.__                      l,/3o a,        &deg;              21-,&#xfd;0         '
TEMPERATURE:                                                                        .
EFFLUENT DESCRIPTIO N:                                                                                 O/tiA WATER CHEMISTRY AT TEST TEMPERATURES DATE RECEIVED:                                   INITIALS                                         '3Dfr\J GLC NUMBER:
* 7'.Qi o3i9                    a. o.Co TEMPERATURE                                                                                                       L. -                    c-
  ,pH                                                                                        k,1I~3             ca1u DISSOLVED OXYGEN. (mg/IL)                           ,                           3'-       ..           .l             .           lI       )
CONDUCTIVITY (gmhos/cm),                                                     "f5.''kLr.                 &        k HARDNESS       (mg1/L)                              *,3iAo                   .LLL                ..            A..              "*..
ALKALINITY (mq/L)                                                  ..
TOTAL CHLORINE (mg/L)-
TOTAL AMMON IA.(mgIL)                       ______                      ______                      ____________
Check with project manager to'see if necessary.
Hardness:>                                                             Alkalinity: "
S10.
End mL: L3                        End mL:. (4.o(                    f,0     EndmL:-                              EndmL:
mL:1)
Start mL: 3"1.                    Sta.rt mL: 4j&sect;                  ik.*J     .Start mL:.          Q              Start mL-';                s'.'  14, [o Sample Volumi*e:.___          Sample Volume:.                      Sample Volume:           S)        Sample-Volume';5 5g*

FISH 96-HOUR STATIC ACUTE TOXICITY TEST WITH48-HOUR RENEWAL TEST MATERIAL:_______                                                          NO.'FRYtCHAMBER:.              10:
10*                                                            P'HOTOP.ERUIOD   (L:D0),
PROJECTNHUMBERI.__1                 2.Q 00-                                       AGE/SOURCE OF FRY:                .Itl:tlc.i,                                                  LIGHT INTENPSITY    (lux):
VIA k!'P in t
                                                                                                                                                                                                                  /-ILC TE-STSPECIES:.       I II .    .       ...
WATER:         C           0A-i "iCUTION       .y..... CX",          -                  T"ESTTEMPER'ATU1RE       ('C):
0
______________                              .o1
                                                                                           ' c              g           -..             P.T
                                                                                                                                          .        '2     .. t*-        213+
DAYIPiAL                                 -      ~                                 i *e~
* ________-. *
                                .REPLCAT                           E                   C                                                                                                                    2                 IW
_   _    __-_           _,W                                       I       ___       _     _o                     _"      .          ... _  _...                ...
_,_   _-,,r                                         _ _,_,_3**             _ _ __.                      _--_            :* i         _ __..                 i
                                              ~~~~~~~~                                 Cz   I_                                                         _          ~I_                       '                                  _           _
__       _ _     _   _   _      _   _                   &#xa3;4-                                                                                                                                         4-
                                                                                                                                                        -                                                             7"-         I PH
                                                       .74.7
                 .....N++* . ...
_...._...._______LA+&deg;
                                                    -.                        T :Y I_ _ _.,,_
___* +
                                                                                                                             ++.
                                                                                                                               -I I'' .../' , '&deg;-__
____;0
                                                                                                                                                                            '&deg;, f Ik  * .i,*
j - /,
1\-KC
____:0*T ,b
__               ___l'   ,_1_F L+!   _
                                                                                                                                                                                                                                               +
                                                                                                                                                                                                                                                    ++
                                                                                                                                                                                                                                                           +
I Q+>
1%          1            17                                                                             ,.'_        _  _
                      *=,+..
                 >,__,__.______,++,,.
ON LL N+:
3, +,
                                                                              %;I_,__                       < 1 1- 10 / ___ 1't ___, I j,
                                                                                                           -10
____                     -JQ" __
11
                                                                                                                                                                                                            )  _
_________,0,      "*                __   ,i"   ___   . __ *                        [.         ,!. ___            e         _    _      _   .+ _         I            9              ~i Obiervstio.n Key Dale:     I         SI lNOl                                 I N - Norm~al                                  PM:-Particulati Malter            I " Imnobilized                                                                                                                                           /
EAR."~aicS~nvi                               FS'- Film on Surface                                                                                   Reviewed By*.,
                                                                                                                                                                    '"*1 ' t*.]+*                  y  ,%AA  LVo-J

DAPHNID 48-HOUR STATIC ACUTE TOXICITY TEST Great Upes Efronrnntal Center                                 f)
TEST MATERIAL:               .         c...                                TYPE OFTEST:                                                              bILuTIONWAtER: \P.ke       C,                1      0 PROJECT NUMBER:-       9-- 1    *     ) 0.(                               NUMBER OF DAPHNIDS4HAMBER:                   5                            GLO ANDIOR BATCH NUMBER:                    .10A      \u
                                                                                                                  #~ ibvry          o                  TE4ST TEMPERATURE:
* Cc TEST-SPECIES:                                                              NUMBER OF CHAMBERS:
INVESTIGATORS:.                                =                          AGE OF DAP.HNIDS:       e>     \\V]Lijo                                   INCUBATOR      #:L .PHOTOPERIOD.112.
CONTROL            A,] 1.6.7-590                                  1..*oqLCONTROL.. F                                              ~         *& 0*:,:
DATE DAT TREATMENT    LEVEL                                                      &#x17d;LiL2J                                                                              0                      2 '1 3 1 2         1..                                21           1 TEST        TECH.             ..
1.. 1 3     "i                        .4                                                                                 L TIME    .. DAY ,. INIALS         REPLICATE NUMBER
_:::_:_                                     C>,___
TEMPERATURE                     0 ,5'.
                                                                    ,C)          J                    _.
pH                                      O*              ~                                          _        _                 I &#x17d;                       _           _              _        _
* DO(gL)                   _   _        _       __
(pmnhoslcm)
NUMBER LVE                                j~V11            1_                   _I                                        1~~    f SooPH                                                                            %   L                        -                                   T           .      .       .                     _.,_
DO (mgJL)                                           ___        (a,                                            _                     ;               __ p_:3_                      .9
___  ___         ___         TEMPERATURE (-C)                    q                _                                      (
  ,                         /.,.....OBSERVATIONS 0
DO  (mg/L)                                                3L(.                  1L                     &#x17d;                                                          -
S.ONDUCTANC          __      _T__                      2                _     _   _     _  _  _    _    _,3                   c                     ~         f          ____
TEMPERATRE (C)            _      _        _             _    _
Observation Key:
PM     Particulate Matter                                                                       REVIEWED BY.                            i       I DOB1- Dried Out opn Beaker ERR - Erratic Swimming                   FS - Film onSurface
                                                                                                                                                                                 'DATE:
C       F- Floater                            IMM . *Immobile                                                                                                                                      t-     I,   t

FISH 96-HOUR STATIC ACUTE TOXICITY TEST WITH 48-HOUR RENEWAL NO. FRY/CHAMBER:;                                                     :PHOTOPERIOD (L:D}:             L..1 T ESITmMAT
       -        E
                .1   A.
AGE/SOURCE OF *FRY:                                                    ,LIGHT INTENSITY (lux):.
PROJECT     NtUMpEpk:   I...b-1 DO:.2
 &#xfd;TESTSPECES:           F   1                                                           OiCUTION WATER:      iZ&~    DH~Y~                                    .TE-TTEMPERATURE P'C            lJ-I yr.,
                                                                                                                                                                                           ,aQ"
                                        +--+0..M"&deg;WE                                            . +oCI'  2: 1: 7 1*          I-
                                                                                                                                                      &deg; 1'&deg;I
* I 1' L,--                                                                                          ,+ "                                         _-._,               ID    I
________ S     .      SOOq (C)~d                          Ds T..P.t.C                                  ____.__        _____,  .    .. __,,____
PH go I.,o                                            IL
                                                                                                        -7                                         --                     -7                                     ?
P.
                                                       '+           +,2L,"                                                                 _      1I_..        _...
_____Il-____
hoI/II'               ... Vd               __jw4                         j I__         VC   ll     __'
                                                                                                                                                                                                        }
Obse,+aI'o' Ke+                            ...          .     .___..._____________________________________l N - Nomal                               -PM - Particulate Matler FS- Film n' SuJrface I, Irroabilized                  ,.I                       a(-I ReieU ERR - Erratic S~winoin If                              (J

i TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV FOR REFERENCE, CITE:
HAMILTON, M.A., R.C. RUSSO, AND R.V. THURSTON', 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7): 7i4-7.19;*
CORRECTION 12 (4) :4i7   '(178)
DATE:    12/1/06              TEST NUMBER: 1861-00                  DURATIONi:   48 HOURS CHEMICAL:&#xfd;   30 MINUTE MEXEL 4 MG/i g1c3009                         SPECIES:   FHM RAW DATA-:
CONCENTRATION(PERCENT)        6.25   12.50   2'5. 00   :50.000 100.00 NUMBER EXPOSED&#xfd;:                 20      20        20        2.,0   20 MORTALITIES:                      0       0         6        20     20 SPEARMAN:-KARBER TRIM:                 .00o%
SPEARMAN-KARBER ESTIMATES:        LC50:              28&.72 95% LOWER CONFIDENCE:              24.91 95% UPPER CONFIDENCE:               33.10

TRIMMED SPEARMAN-KARBER METHOD.            MONTANA STATE UNIV FOR REFERENCE, CITE-HAMILTON,, M.A.i, R.C. RUSSO., AND R,.V. THURSTON,, 1977.
TRIMMED SPEARMANA-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL.. 11(7): 714-7"19, CORRECTION 12(4)1:417      (1978)1.
DATE:     12/1/06                 TEST NUMBER-: 18.61-00                    DURATIN:     9,6 HOURS CHEMICAL:. COOK NUCLEAR 30 MINUTE SAMPLE                                  SPECIES:   FHM
:RAW DATA:-
o).16"d" C71r.yj o.0     -i.        -,*      ,.
CONCENTRATION (PERCENT),            6.25:    12.50. 25.00'   50.00 1oQ.00 NU.MBER EXPOSED.:                       20       2.0     20          20     20 MORTALITIES:                              0        .0     7          20     2.0' SPEARMAN-KARBER TRIM:                           .00%
SPEARMAN-KARBER    ESTIMATES:;           LC50:             2 7 . 7 4-    6 95% LOWER CONFIDENCE:
:95%&#xfd; UPPER CONFIDENCE<:                  32.1 42e.         I                6.2

TRIMMED SPEARMAN-KARBER METHOD'.      MONTANA STATE UNIV FOR REFERENCE, CITE:
HAMILTON, M.A.,. R;C. RUSSO, AND R.V. THURSTON.,, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOLt. 11.(7): 714-719; CORRECTION 1,2(4) :417    (1978).
DATE:     12/1/06               TEST NUMBER: 1861-00               DURATION::    96 HOURS CHEMICAL:    COOK NUCLEAR GLC#,7009'2..5 M'G/'l                    SPECIES:'  FHMK RAW DATA:
    .CONCENTRATION:(MG/L)            . 16     .31    .63      1.25' 2-.506 NUMBER EXPOSED:                    20      20     20        20:    20 MORTALITIES:                        0        0     7        2o     2.0 SPEARMAN-KARBER TRIM:                     .00%:
SPEARMAN-KARBER ESTIMATES:          LC50:*            .669 95%. LOWER CONFIDENCE::              .60 95% UPPER CONFIDENCE:                .80

                                                                                                                                                                                                               -'~ ,~
DAPHNID 48-HOUR STATIC ACUTE TOXICITY TEST Gr.Fat Lakos Envlronmontpi.Cdntcr
                                                    .?;o-PAIV r"                                                                                            All I~ANWATFR~      ~ZP(   lA- 1 ()
TEST MATERIAL:                  Ine~                                    TYPE OF TEST.;            ~          1 NUMBER OF DAPHNID               HAMBERFL                                      GLC ANDIOR BATCH NUMBER:
001         nO PROJECTNUMB&#xfd;R:
TEST.GSPECIES:                                                           NUMBER OF CHAMBNERS:               I4I-    +-  1         r &V" ew             TE ST-TEMPERATURE:
INCUBATOR #9 INVESTIGATORS:                                                          AGE OPOAPI-NIDrS:                       1     2.Q 0L                                                   PHOTOPERIOD:
rK     I t~**I    ~. at          15-         Ake, I      LAS
_________                                             I.f) , )t= 11.0In.           (J     ~.)I
                                                                                                                         -->. C?'
5 DATE                                 TREATMENT. LEVEL             CONTROL-                                                                        251 TEST       :TECH.                                    r-rF                     I         14                                            3 4
TIMIE          DAY     INITIALS     REPLICATE NUMBER        i1       +/-2J2j3+/-        1r12       t3        1 40       L4, pH                                                      76____
_____3_                                                 015                        -0     -      -      &#xfd;A OBSERVATIONS-----------------------
DO0(MgIL                                      Z                 i_                      _                   -L               iLf                        q~L/
5   .,
_ _    TEMPERATURE ("C)
NUMBER LIVE,!.         511              "15                                                                             IIIII1                               ~
11f_U~I~
L 0                   ~~~~~OBSERVATIONS              iu..I=L..                                 .
S,                                       2         1 2:                  pH DOm                    Z"ir                      .                                          s SP.CONDUCTANCE                      C0'f0                                            30-3(4 TEMPERATURE (Cp).                                                  ~            _     _        _      _ _ _   _   _        _  _   _L9  _    _   _     _   _    _  _   _ _ _
       -Obs.ervation Key:
REVIEWED BY:,~Qk           n' r Y93~12 DOB-- Dried Out on.Beaker               PM - ParticulateMatter
_..ERR -- ErratIc SwImmIng ::               MM -f IlmmoiSUrface                                                                                                           DATE:           dAi F - Floater                         1MM - Immobile

TRIMMED SPEARMAN-KARBER METHOD.       MONTANA STATE UNIV FOR REFERENCE, CITE:
HAMI.LTON, MA., R.C. RUSSO, AND RIV. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD' FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY&#xfd; BIQASS8AYS.
ENVIRON. SdCI. TECHNOL,.. 11(7)_: 714-7,19;1 CORRECTION 12(4).:417     (1978).,
DATE:    :121/1/066            TEST NUMBER: D MAGNA                  DURATION:'   48 48 CHEMICAL:     COOK NUCLEAR 30 MINUTE                                  SPECIES:  D MAGNA RAW DATA-:
CONCENTRATION (PERCENT)         6.25   12.50 25&#xfd;.00      50.00  100.o00 NUMBER EXPOSED:                   20       -20     20        20      20 MORTALITIES,:&#xfd;                            , 0       0                2.0 SPEARMAN-KARBER TRIM:                      .o00%
SPEARMAN-KARBER ESTIMATES:         LC50:              35.3&#xfd;6'     0,743 95% CONFIDENCE LtMIT.S h~WL ARE NOT RELIABLE,

TRIMMED SPEARMAN&#xfd;*KARBER METHOD;.      MONTANA STATE UNIV FOR REFERENCEi CITE:
HAMILTON, M.A., R.C. RUSSO, AND R.V-. THURSTON, 1977.
TRIMMED: SPEARMAN'-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIbQASSAYS.
ENVIRON.' :SCI. TECHNOL., 11 (7) ' 714 -719; CORRECTION i2(4):417      (1978)
DATE:    12/1/06                TEST NMBER:]i 18861-00                 DURATION,:    4A8 HOURS CHEMICAL:&#xfd;   COOK NUCLEAR 2*.5&#xfd; MG/L gle6#7009                         SPECIES-:, D. MAGNA RAW DATA-:.        ,
CONCENTRATION (MG/L)              .16      .31        *63     P.2 5 2.50 NUMBER EXPOSED:                     20        20        20'      2o    20 MORTALITIES:                         0         0        0.      20    29 SPEARMAN-KARBER TRIM:                      .00%
S PEARMAN-KARBER   ESTIMATES:        LC50.:'                 . 88 9.5:% CONFIDENCE LIMITS ARE NOT RELIABLE.&#xfd; V1

THIS PAGE INTENTIONALLY LEFT BLANK

GuEC
                                 ,September 17, 2007 Mr. Eric Mallen, American*Electric.Power -
Great                    Donald C. Cook Nuclea'r Plant      '
takes,                    OiieCdok Place Enuironmental Biidgman, Ml 49106
        .Gentei:
RE:      ACUTE TOXICITY, TEST REPORT FOR A SAMPLE -
Applied                           COLLECTED FROM AMERICAN ELECTRIC POWER, COOK Environentals                     NUCLEAR PLANT ON AUGUST 28, 2007,                        -
ScienCes"                                                                                    -
Ww~wwglec-onlinecmm

==DearMr. Mallen:==

Great. Lakes Enviroiamiental Center (GLEC) has complted.61 .ui"analyses of tle 48-hOui Dcap ic/i.
        ,dperationr*             ,m"g' .ahd 96rid urfatlead minnow acute toxicity test performed-on.a sample collected by 739 Hastings St.         American Electric Power (AEP) personnelon August 28,2007. The toxicity tests were initia'tod Traverse Clty-            Onl
                                  -. August 29, 20Q.;7.. The~sample analyzed was a Mexeltreated effliuent samplethalt was diluted, M)49686                  3: [(GLC Ntiniber: 7160) at the AEP Cook Nuclear Plant. Lake Michigan water(GLC Number 231 941-2230          .7-16.)     Ccollected by&#xa2; AEP personnel was usedasjthe dilutidn waterfor tlih D. 1)agna a6id.fathead "i231941-2240 fax       . linil ow tests'.
* TFhe-d iluted Mex.eljtreated effluenit samnple was acutely toxic. to~both iD. Incighca.dn'd fatlead ,
bpcratiels; 1295 KingAve.             minnows-. The 48-1iour .D   . agntland96-howrfathead mminow L(Q (median lethal toxicant, Columbus                 concentration) ,rnc D ,)4agncI 48,:hour          (median efwfrctconcetratin'o)'01" estiiiies    eie all o59' OH 432,12          .. perceftefflLient, or 1:5 TIU,, (,acite toxic units) 614 A87-1040          -
614A487-1920 fax         A summary of the tdstrcdditions forithie txicity tests ariiiCluded in Ta.bles land 2. The 48-,
hour and 964hour LC'0 and;EC, estimates and toic ity:test results are. ilcluded'in Report Forms I and 2. The'raw data :are included iii Apperdik      A.,
If you have any.questions or-comnilents coicerning the.esults&#xfd;0ofthese toxicity, tests,,pl'ease contact either me or Dennis McCauley art(2.3 1) 94-1&#xfd;-2230:' Thinik you for the.pporttuity to provide this.selwvice to Americ                Power-.D6naldC*. Cook Nuclear Plant:
i&ElectiPic sincerlely; Mailee&#xfd; W. Gairton.                     .                     ~Deninis J. M6Caule-y
                               'Laboratdry Coordinator                                         Principal :Research. Sc i'e.nist/
-'--a,                                                                                         Sen iorOperation's Manager a'-

TABLE 1, FATHEAD MINNOW TOXICITY TEST CONDITIONS Mexel treated effluent diluted 3:1 Summairy of-Toxicity Test Conditions 1.. Test Species and Age:                             Pinhephales promelas, (Fathead minnow),
4 days-Augugst 25, 2007 2>. Test Type. and Duration:                         96-houre Static, With. renewal ai 48 hours 3.Test Dates:                                         August 29-September 02, 2007
: 4. Test Temperature (CC)-.
2
 .5. Light Quality:                                     Ambient Laboratory, 10-20 jiEI'mIs-
: 6. Photoperiod:                                        1.6h light, 8.h darkness
: 7. Feeding Regime:.                                  48 hours
: 8. Size of Test Vessel:.                               250 imL glass beaker
,9;   Volume and Depth of Test Solutions:               2N0 mL,,65 mm
: 10. No. of Test Organisms per Test'Vessel:             10;
: 11. No. of Test Vessels'per Test Solution:             2
: 12. Total No. of Test Organisms per Test Solution:     20
: 13. Test.Concentrations (percent):                    100, 506,25, 12.5, and 6.25
: 14. Renewal'of Test Solutions:                       48-'hour renewal' 1:5. Dilution and PrimaryControl Water::             Lake Michigan GLC# 7161 16.. Secondary Control Water;                         Synthetic Laboratory (Moderately Hard MH# 1543)
:17. Ae.ration:                                        None Mortality ([050)*
: 18. Endpoints Measured:
                                                                                                       --10.

REPORTING FORM I FATHEAD. MINNOW ACUTE TOXICITY TEST Mexel treated effluent diluted 3':1 Facility Name: AEP Cook Nuclear Plant                                                                                 NPDES'Permit No.,:
Receiving Water-: Lake Michigan                            Outfall__                                                  RWC: N/A Test'Dates: 087/29/07"- 09/02/07                          Test Speci.es'. Fathead minnows                             Age Range: 4 days old TestLaboratory: Great. akes Environmental Center.(GLEC)                                                               Report Date: September 18, 2007 BULK SAMPLE INFORMATION SAMPLE,               DATE,              ARRIVAL             DATE OF            ARRIVAL     DECHLORIN           ARRIVAL         ARRIVAL         ARRIVAL COLLECTION            RECEIVED          TEMPERATURE            FIRST USE             TRC          ATION?.               pH              DO         AMMONIA DATES-
: 1.    &#xfd;08/288/07           08/28/07'            06.C               08W29/07,             NM             No                8:.35-           10.1          NM What'test methods.were used: EPA/600/4-90/027 and EPA-821-R-02o01i Describe'any deviations from test-methods:               None Source aftest organisms: In-house lot.# 08/25/07 TEST ,DESCRIPTION Fed/Un'-fed:, Fed                                    Food/Feeding Frequency: Artemia naunlii.2 hours prior to,48-hour renewal No. .eplicates per.                                  Nd..O'rganis~ms per Concentration:: 2                                    Replicate: 10                              Effluent Filtered? No.
Effluent Sample Type!: Sample1:.l. Composite                                                    Sample 2:,
Diluent (0i): Lake Michiqan"Water (GLCW#7161)                                                   Secondary: Control:(02): Synthetic Laboratory Water (Moderately Hard) MH#'1 543

==SUMMARY==
OF RES.ULTSt 48-hour LC5o: 68.3%
96-hou r LCgo: 65.9%
Percent Mortality perrConcentration (Percent Effected p'er Concentration)
                                 --controis&#xfd;-                                                       Effluent Concentrations--
Day 01                      A-.25 Percent        12,5. Percent     i62Percent          soPercent,      _i6o"Percent            Peroehtn 1              0(0)                                 0 (0                0:(0)           0o0o                5 (5)            100 (100) 2              0(0)                0(0)             0(0)                :0(0)           0(0)                5(5)             100 (100)
          .3              0(0)                0(0)             0(0)'               .5(5)           0,(0)               5(5)             100 (100)
:,              0:(0)               0(d)             0(0)                 51(5)          0(0.
: 0)               5(5)             10 (.. 00)         ____0 Raw datasheets are inctuded in Appendix A, Synthetic Laboratory-Water (Moderately Hard)
.02:o
 !nr'ettigato0: MaileeW. Garton                              'Contact:
IennisMc~                    aule,                      PIhone     No.: (231) 941L2230 Principal Research Scientist/Manaoer-of Operations                  Date Signature                                                    Title
_Titlei                                                      Date,

TABLE'2' DAPHNIA MAGNA TOXICITY TEST CONDITIONS Mexel treated effluent diluted 3:1 Summarv of Toxicitv Test Conditions Summarv of T oxici tv Ttest ConIditions 1 ,, Test Species and Age:                              Daphnia magna, <24 hours old; 2.
Test Type and Duration:                            Static, 48.hours&#xfd; 3:    Test Dates:                                        August 29-3:1, 200.7' 4;,
Test'Temperature (C):                              25+/- 1 2
: 5. Light.Quality:                                     Ambient Laboratory, 10-20 1aEIm 1s
: 6. Photoperiod:                                        16 h light, 8Bh darkness
: 7. Feeding, Regime:                                   None:
8 .
Size .ofTest Vessel:                               3OraL plastic cup, Volume and Depth of Test Solutions:-                15 rL, 20 mm 10:. No. of Test Organisms per Test Vessel:.             5
: 11. No. of Test Vessels per Test Soiution:               4 12.&#xfd; Total No. of TestQrganisms per Test Solution:      20
: 13. Test Concentrations (percent):                      1:00, 50, 25, 12.5. and 6.25
'44. Renewalrof Test Solutions:                         None 1:5. Dilution and Primary Control Water:;.               Lake Michigan GLC# 7161 1.6. Secondariy Control Wate:                           Synthetic.Laboiatory (Moderately Hard MH# "1543) 1:7. Aeration:                                           None
: 18. Endpoints Measured:                                  Mortality (LC") and Effect,(ECo)

REPORTING FORM 2 DAPHNIA MAGNA ACUTE TOXICITY TEST Mexelrtreated effluent diluted 3:1 FadilityNarhe: AEP Cook Nuclear Plant                                                                                    NPDES Permit No.:_
Receiving Water: Laeke Michigan                             Outfall:.                                                    RWc: N/A Test Dates: :08/29/07 - 08/31/07                            Test sPecies: Daphnia.mana                                   Age Range: <24 hours Test-Laboratory: dreatLakes.Environmental Center (GLEC)                                                                  Reppo .Date: :September 18, 2007
                                                                 -BULK SAMPLE INFORMATION SAMPLE                DATE             ARRIVAL              DATE"OF           ARRIVAL      DECHLORiN             ARRIVAL     ARRIVAL        ARRIVAL COLLECTION            RECEIVED        TEMPERATURE             FIRST.USE            TRC,           ATION?                pH          DO         AMMONIA DATES
: 1.      08/28/07             08/28/07,          046CC               08/29/07            NM                No,               8.35         101           NM ND:, Not Detected.
wha~t testmrethods'were used: EPA/600/4-90/027 and EPA-821-R&#xfd;02-012 Oescribelany deviations from test:methods:                None Source of test organisms: In House: .MH'08-17-07.
TEST DESCRIPTION Fe,dIUn-fed:' Un-Fed                                 Food/Feeding FrequencyNone No. Replicates per                                    No.,Otrganisms per Concentration:.4                                       Replicaite: 5                              Effluent Filtered? No Effluent Sample Type: 'Sample -1:,Composite,                                                     Sample2:.
Diluent (0)--Lake Michigan Water (GLC# 716t1)                                                    Secondary Control (02): Syhthetic LaboratoryWater (Moderately Hard) MH#.1543

==SUMMARY==
OF RESULTS*
48-houf LC56d65.9%
48-hour ECso: 65.9%
Percent Mortality-per Concentration
_  -(Petrcent                                            Effected &#xfd;per Concentration)
I
                                  -controls--                                          ...         -Efttluent&#xfd;Concentraoions--
bay
   .....         ._____d_                                   .6.25 Percent    i   z: Perc:enht     15Percerit            'd Pecent      _io Perceh    _     Perdeht b'010)             0()               o-(o)              o.(o):           0o(o)                 0:(0)         95 (95) 2               o (0)             0(0q              0(0)               0(4)             op()                10(10y         100( 100)
*"awdata  snheets are included in Appendix AW Investigator: Mailee W; Garton

==Contact:==
Dennis McCauley                                      Phone No.: (231)941-2230',
Prinidoal Research Scientist/Mainaner; nf;iOneations ScientistIM6naage of;Oh6rations Signaturet                                                     Title Research Principal                                                               Date 13.

Appendix A Raw Data Sheets 44 ~ J ~  4

CHAIN OF CUSTODY RECORD (TO BE COMPLETED ONSITE AND                         ISUBMITTED WITH SAMPLES) 739 Hasilngs Street Traverse CityMI 49686, Phone:' (231)j941-2230 Fax: &#xfd;(231) 941'-2240 Facility:-~~                     '--~~Q~~                                                                   Collec tor          ~~444%/                   ~~~L/I'~~/aJLL*
Location.        *   .1    &x0,    Z   , le' .                  ,                                                 Date            / 7
* 9~~/-,
Contact Person:          t    U    P-'c llc,. .                                                              Witness            IT-f ~     f"K)~f t/( -,~
Phone*-Number:           6Sl-     'AG5,&#xfd; 5-'7ID   'X,/.lI  D                                                  Date:             'e-k'-   /6,'-
GLC NUMBER (Lab ID)
SAMPLE IDs DATE/TiME OF SAMPLE*
VOLUME COLLECTED SAMPLE CONTAINER DESCRIPTION.
(Type.of sample, source, physical PRESERVATION 1 ANALYSES-REQUIRED Additional Parameter.s, Measured atCCollection
                   ,                                                                        characteristics)                                               Ammonia          Chlorine
                                                                             .... __     __I,/,.LmgIL
____    ___     ___   ___   __     __                                                          J
     ,             cY->o7.         S/i,~gW 1L.           / ,,,.//a..   .*t.*                *L     *4Ar               ,__,___,M__-__
* or 24-Hour Composite sampl.es,'please indicate time~s and. dtates thee sampling :start~ed :ahd ended.
TRANSFER OF SAMPLES:
(First::signature is samn-pler, last signature: is *authorized Iabgeatory ep.resentative)
SHIPPER                                 RECEIVER                          DATE                              TIME '    /df     TEMPERATURE
: 2.      1/*iz. ~,,.l                                   ?.*
-j     Condition. of Sample Upon Receipt:                                 . 2      "                                   0 ,                                   Received-o   Ie.

G I.EC                                 EFFLUENT AND" RECEIVING WATER CHECK-IN FORM Ukes Envikompenia Center Gre-it Lv CLIENT:      CcOS: . )- ;          Y    --\r                              PROJECT NUMBER:,~                  \(-
INVESTIGATORS:
INITIAL WATER CHEMISTRY (UPON RECEIPT)
WATER CHEMISTRY AT TEST TEMPERATURES SDATE RECEIVED:-               167          INITIALS                              Z GLC NUMBER:                                    ~?U_____
  'TEMPERATURE.257e.                                                     c)O                          _          _
pH:~6J                                                                      I       ~         I     _       _   _
DISSOLVED OXYGEN (mIL)                                       (0.                  I            ..        .
CoNDUCTIVITY (Mumhos/cm),                                   '_,    07 HARDNESS (mglL)-
ALKAUiNITY (mglL).______
TOTAL CHLORINE (mg/L)'.                                      to 1__A TOTAL AMMONIA (m/ L)                    _        _       _      ___              A"i"J               "_*
Check with project manager to see if necessarY.
7      (   ...        Hardness:
* i                                   Alkalinity::           7t End mm"L >'7                     End mL:                       End mL_:____               End m*":____
Start mL:I                       Start mL:js71        ,        Start mL:                  Start mL: O .
Sample Volume:. &#xfd;/&17L       Sample Volume:!      L     Sample Volume:
* Sample: VoIume;_Q. L-

GLECE                                                                )APHNID 48-HOUR STATIC: ACUTE TOXICITY TEST Grot: bLkes EnIronmital: Con tur TEST MATERIAL:    (00h         Atehrue-                                      TYPE OFTEST.j~e~\ChS                                                 DILUTION WATER:'k4            &#xfd;     F,    'ZLe'          c)
  -PROJECT.NUMBER:-                       -                                   .NUMBER OF DAPHNIDSICHAMBER:_                 _     _        _       GLC AND/OR BATCH.NUMBER:
TEST SPECIES:          -                 ,,                                  NI.UMBER OF CHAMBERS:          /             -                      TEST TEMPERATURE:       P      O     ,(        !
INVESTIGATORS:%            2L    -           .     ..                  . AEO.FDAPHNIDS:-t'..      [IfI,,h       k' 'l(f                      INCUIBATOR      #:i     PI-ITOPERIOD:/
DATE                                 TREATMENT LEVEL                CONTROL            P\G.''k                     2.%            c z-7                                  (citij.
TIME1        OY        iIiL         REPLICATE NUMBER         1                                &#xfd;1                 1'2     3     4     1       3 F2       1112FFT  F                                        .
TEPRATURE (*C)                                                                           '2j-                                     __         _      _          2-'s -s 0 0 (m g /L)                                            _    _   _         _   _     _   _     ~     I i               c kO_
                                                                                                                                                                , q_                _    _      _       _  _    _ _
_______1            (moslcm)
NUM13ER LIVyE              >
s1I~6                                        1       ~~i                               ~          .5 OBSERVATIONS              _____                                       -       F            J Ji                   __I 1               ~~~~~pH:f$                                                 (1_                                       _      _
____                      ~~~~~TEMPERATURE (C)______                                                                                   7            t                                        __~12 NUMBER LIVE                _     -j   11      1Z                                                                                    0 YZs14 AOBSERVATIONS                                    __W                    I____f_-
_DO   (mgL                                           __       __              _      _     _
SP. CONDUCTANCE                                                                  32                3zaa.
___   ~       ~     __ ~      EMEATR               _      _    DH__
_      -I__
_                  1      ~          ~                 '4X                _      _  _        _      _     _        _   _     _
     'Observation Key:
DOB .Dried Out on Beaker                  PM     Particulate Matter                                                                     REVIEWED I ERR - Erratic Swimming                   ,FS - Film on Surface
-J       F- Floater                             IMM-- Immobile

TRIMMED SPEARMAN-KARBER METHOD.            MONTANA STATE UNIV FOR REFERENCE,       CITE.:
HAMILTON, M.A..        R.C. RUSSO, AND R'.V. THURSTON, 1977.
TRIMMED SPEARMAN.-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7).: 714-7-19; CORRECTIQN412.(4)A:417 . (1'978).
DATE:      58/29/027                                                   TEST. NUMBER:. 1861-00 CHEMICAL: ,COOK NUCLEAR-AEP                                            'SPECIES': D. MAGNA RAW DATA:
CONCENTRATION (PERCENT               6.25. 12.50    25 .;00 50.00 lOO..00 NUMBER EXPOSED:                        20      20
:20    20      20 DURATIIN (HOURS                   LC5O       LOWER 95% LIMIT       UPPER, 95.% LIMIT    PERCENT TRIM 48                     -65. 98              60 .12                72:-.41
.00

FISH 96-H.QUR STATIC ACUTE TOXICITYITEST WITH 48-HOUR RENEWAL TEST MATERIAL:                    /V A         a.L K,                              NHO. FRY/CHAMBERl:        10                                                        PHOTOPERION (i           )       .Am16_;8 i PTOJECTPE    N             HUMEER:__
_____                  GLCII_      -' 1r(1       AG&deg;ISOURCE OF FRY:     7      qI,            .       ,      c-      L C?2              ,LIGHT. INTENS1ITY   (lux):   .      Am~bient TEST-,SPECIES:         I:FHM                                                       DILUTION WATER           M"Had ~lq~        ?c..-I                                    TEST TEMPERATURE (!C):                 25&deg;+/- 10C TEST            TECH.                  TREATMENT LEVEL,    _____        ______Q      A    '&#xfd;i1                    /z~5t&#xfd; 1                  ____                 eL                                    "     ci       '"r DATE OAT,          PMEL                    REPL. CATENUMBER,         1            2
                                                                                                           -250                     25.0                  250                     2s50                           25             25.0 T.-p.e.-IIC)                      25.0
                                                               ,                                                                            ,                      -                             _               __o 35o     I              ___o 0.....
        'I         _   _  , __lsrl  _Sp.               Coow*G, 0n*    )             "    _,-" ",7_   _
4______ur___"_,,                                                                    ___.'
                                                                                                                                                                                           -  'I~                   (                0'"t   ___
7 48-Hour.       -1
* DO
                                                   " bs*a o* tCli    .
77 2520 t ._       I ....0 12.01 11        25,0
_1 no
                                                                                                                                                                                                                )~Lj7 I/    ...
                                                                                                                                                                                                                               ),1J I_   _
Chem1&#xfd;1Tries.0                         DOV&#xfd;g&)
mIS                                                                                 I
_                         -                                               -//                                 ,- ,                    m m,--
__________-,;,I  ___
1__)I-                                            _
IC                 1                                                                               J_        .I         _     _ _
H- KI F flCI      >     _____                      _
2    IT~~.I~I SoIICn,,       I ,.I        ,                                                                                             ...
_Hlg_1____
I           [1_&#xfd;  O"Z fIT   .WoI.I0.IC Nw5be, 1:14 I__
(S'.
IX-
                                                                                                                                                                                        "Y' 1____
PHrvI~n                        __33__
ObnSUIaNI Ken.
Dawl:       Lj~q~Q')
* Ne.,I,2I
     *E,.nIn,Sa.nwxno M.- P2Iicui2ltR M.1D1W F'S TIiln,,on Sw"lac&#xfd; I- Imnwe&h1ed By.                  ~~~,)            cu rJ

TRIMMED   SPEARMAN-KARBER, METHOD.. MONTANA STATE UNIV FOR REFERENCE, CITE:
HAMILTON,&#xfd; M.A., R.C. RUSSO, AND R.Ve. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL :CONCENTRATIONS IN TOXIClITyBIOASSAYS,.
ENVIRON. SCI, TECHNOL. l(,7): 714-719; CORRECTION 12:(4) :4-17 '(1978).
DATE:    8/29/07,                                                TEST NUMBER: 1861-00:
CHEMICkL:,COOK NUCLEAR. GLC7Ei60                                 SPECIE'S, FHM:
RAW DATA:
CONCENTRATION(PERCENT            6.25. 12.50    25._00 50.00 1O0.o00 NUMBER EXPOSOED:                   20       20       20    20'     2'0 DURATION    (H       Y       LC50       LOWER 95%   LIMIT    UPPER 95% LIMIT    PERCENT TRIM 48-                 6.8.30               63.84               73.08
 .00

TRIMMED. SPEARMAN'-KARBER METHOD.        MONTANA STATE UNIV FOR REFERENCE, CITE:
HAMILTON, M.A.,      R.C. RUSSO, AND R.V. .THURSTON, 1977.
TRIMMED SPE&#xfd;ARMAN-KARBER METHOD FOR ESTIMATING MEDIAN LETHAL CONCENTRATIONS: IN TOXICITY'BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7"): 714-719:;
CORRECTION 1:21(4)':4.17  (1978),.
DATE:     9/4/07                                                       TEST NUMBER: 1861-00
  &#xfd;CHEMICAL: COOKNUCLEAR-AEP                                             SPECIES:: FHM RAW DATA:'
CONCENTRATION'(PERCENT             6.25   12.50    25.010  50;.00, 100.00 NUMBER EXPOSED!:                     20       20       120     20:     20 DURATION     (HOURS.            LC50       LOWER  95% LIMIT&#xfd;      UPPER &#xfd;95% LIMIT   PERCENT TRIM 72 64, 96,                   65.'98               59 .!93
.00

THIS PAGE INTENTIONALLY LEFT BLANK

D. C. COOK PLANT ZEBRA MUSSEL MONITORING PROGRAM WORKSHEET Mexel Test - Art. Sub. Size and Density Calculation Sheet Date:   9/13/2006 Sample       Mexel - Untreated            Mexel - Treated                       Mexel - Untreated              Mexel -Treated VeliQer Micrometer      Size         Mrter           Size                             # of veligers               # of veligers Number         Reading         (u)        Reading           (U)                               per slide                   per slide 1             2            78            2              78                 Slide #                    Subsample           3 2            16           627           2.5             98                    1               1             1             1 3             3           118            4             157                    2                            2              1 4             4           157            2              78                    3                            3              2 5                                        2              78                    4               3             4             3 6                                        6             235                    5                             5 7                                        9             353                    6                             6 8                                        3             118                    7                             7 9                                        2              78                    8                             8 10                                        3             118                    9                             9 10                            10 T12 12   _______
Avg.                         Avg.
13                                                                         #I slide           0.8       # / slide          2 14 16 17 18 19 20 21 22 23 24 25 26 27 28 29 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Avg.                        245                           103 Min.                         78                           78 Max.                        627                           353 Dens.                        427                          1067 E. Scott Rose          / 9-16-06                                              ?A i~4~ 6 Analyzed ba         Date                  Reviewed by                       Date

r; i~.   ~~
* EII1    LIi 6.6 OA l2 IJ!
MWIASl b
                                                               ~iIF-.l rI 0
tf.,lMl, am nO1' D'
                                                                                       "'dLV.
D~      Ul~ilrllh AMIOVOUEC*T tAI-       i .I ,
Mexel Test - Art. Sub. Size and Density Calculation Sheet Date:      9/28/06 Sample  Mexel - Untreated                Mexel - Treated                      Mexel - Untreated                          Mexel - Treated Veliger MicroMeter          Size       Microaeter         Size                                #tofveliers                            #of velgers Number   Readig              (U)         Reading           (u)                                  Per slde                               per side_
1       12              470            10             392                   Slide #                                  Slide #
2        6               235             8             314                       1               5                        1             1 3        11              431             8             314                       2                                        2            5 4        10              392            13             510                       3               2                        3            9 5        14              549           2.5              98                       4               1                        4            2 8        9               353           2.5                8                                                               5 T       2.5               98             6             235                                                                6
                                                                                   -7                                        7 8         8              314             8             314 8
   &sect;        2                78            11             431 9
10       2                78             3             118 10                                       10 11        8              314             7             274 12                                       8             314                    Avg.                                     Avg.
13                                        2              78                 # I slide           2.2                   # / slide        3.4 14                                        7             274 15                                        9             35-116                                       3             118 17                                       9              353 18 19                                                                      Samples pulled from Mexel test equipment 20                                                                                                        on 9-28-06.
21 22 23-24 25 26 27 28 29 31 32 33 34 35 30 37 38 39 40 41 42 43 44 45 46 47 48 49 50 Avg.                     301                            270 Min.                                     787 Max.                     549                            510 Dens.                    1173                           1813 Analyzed by I /A4~t93 Date c*A~&#x17d;~~
Reviewed by 7/4;~,
Date 3Lf

D. C. COOK PLANT ZEBRA MUSSEL MONITORING PROGRAM WORKSHEET Mexel Test - Art. Sub. Size and Density Calculation Sheet Date: 10/12/06 Sample  Mexel - Untreated            Mexel - Treated              Mexel-Untreated                        Mexel-Treated Velicer Micrometer      Size        Miomar            Size                     # veligers                           # veligers Number   Reading         (u)         Reading           (u)                      per slide                            per slide 1        9          353             8             314         Slide #                               Slide #
2       13          510             7             274                           4                       1           7 3      6.5          255            10             392             2             1                       2           94       A.
4       10          392             2              78             3             2                       3            7 5      21           823             2              78             4          -2                         4           15       B 6       8           314           2.5              98             5            15                       5           17 7       2            78             9             353             6                                     6 8       3           118            11            431              7                                     7 9      28          1098             9             353             8 10      20           784           2.5              98             9                                     9 11      30          1176            18             706             10                                   10 12      57          2234            12            470            Avg.                                 Avg.
13      14           549             9            353          # I slide       4.8                 # I slide         28 14       8           314             8            314                                                                             A 15      2.5           98            21            823                                               Found 2 "clumps" of vellgers. One with 65-75 Individuals 16       3           118             8            314                                               ranging in size from 10um and the other with 8-10 17      2.5           98            12            470                                                   Individuals in the same size range. This total of 94 18     25             98             2             78                                              includes 70 and 9from the "clumps" as well as 18 others found on the slide 19       3           118           2.5             98 20Td_                                10            392                                                                              B 21                                   19            745                                               This total of 15 includes 2 smaller "clumps" of 5 veligers 22                                   12            470                                                             plus 5 others found on the slide 23                                   14            549 24                                    8            314 25                                   15            588                       Mexel Carbon Steel Samples 26                                  2.5             93          One Test and one control carbon steel coupons were removed from 27                                   2              78         the treatment system and analyzed. The coupons are 1/2Iwide X-6" 28                                  3.5            137           long. 3 ( 1/2' sections (.75 in2 total area) were viewed under the 29                                   6             235             scope. Settled veligers on these 3 sec Dlons were counted and 30                                   9             353                                         measured 31                                   2              78                                          Untreated Coupon                       Treated Coupon 32                                   2              78 33                                  2.5             98                                  Section 1 Section 2       Section 3 Section 1 Section 2        Section 3 34                                   2              78             #post-veligers           71           14           30           18           3          5 35                                   8             314                                    1,497       1,449          725          725         676         290 36                                   9             353                                     483         676           531          242         290         290 37                                   2              78                                     386         821           966          483         290         193 38                                  10             392                                     386         580           869          386                     386 39                                  11             431        Randomly chosen p           1,449        628           386          290                     24-242 veliger size 40                                   2              78                                     725                       918 measurements(um) 41                                  2.5             98                                     628                       435 42                                    3             118                                     580                       531 43                                  3.5            137                                     676                       773 44                                   2.5             98                                     918                       580 45                                                                 Avg. Size (um)          773         831           671          425         419         280 46 47                                                                  Density ()            440,200      58,800       188,000      111,600      18,        31,000 2
48                                                               Avg density (#/M )                  237,667                                 53,733 49 2
50                                                                                      Densities based on 1550in /M2 Avg.                   501                           284 Min.                   78                           78 Max.                  2234                           823 Dens.                 2560                          14933 E. Scott Rose//jf,'/ 10-12-06 Analyzed by          Date             Reviewed by                 Date

D. C. COOK PLANT ZEBRA MUSSEL MONITORING PROGRAM WORKSHEET Mexel Test - Art. Sub. Size and Density Calculation Sheet Date:
Sample    Mexel - Untreated             Mexel - Treated                                                  Mexel - Ireatea Veliaer _____           ____          ____          ____             Mexel - Untreated                        (all omi nisms) mbmer            Size      Mkcrmeler          Size                     # of veliger                           Sof veligers Number      Re*ding          (u)        Reading           (u)                       per side                              per slide 1        11.5           555            2             97           Slide #                            Slide #
2          22          1063            15           725               1            10                    1               58 3           7           338            2             97              2             31                    2                12 4          32          1546            8            386              3             17                    3               20 5          21          1014           1.5            72              4             12                    4                6 5             1f4                   5               32 6            9          918            3             145 7           8           386            2             97              6 11           531            2             9                                                   7 9          23          1111           2 17           821           1.5            72              9                                   9 11          15           751                           2             10 12         46           2222           11            531            Avg.                               Avg.
13          13           628            6            290          0 1 slide        16.8              # I slide           25.6 14           2            97            5            242 is           a           386            7            338 16         2            1014           19            918 17           15           725           14            676                                              Mexel - Treated 18          14           676           16            773                                                 (settled o _anisms) 19           19           918            6            290                                                             # of veligers 20           1i72s8                                     7                                                               per slide 21            F           386           14            676                                              Slide #
22           17           821           16            773                                                  1                10 23          1I            918            11           531                                                  2                12 24          13            628           17            821                                                  3               19 25           9           435            18            869                                                  4                5 26           12           580           22           1063                                                  5                11 5
27          93          4492            18            869 28          15            725           11            531                                                  7 1014           11            531                                                  8 29          21 30           9           435            22           17=3                                                  9 31          7                           T9 15           72-                                                  10 32           7            338            8            386                                               Avg.
33           1            531           25           1208                                             # / slide           11.4 34          18            869            5            242 35          14           676           2.5            121                                         Density                      6080 36           5           242            29           1401 37          45          2174             8            388 38          20           966            18            869                                         >half of the organisms on the treated 39           6           290            56           2705                                         slides were less than 170um in length, 40          14            676           15            725                                         i.e. still considered trans-locators 41          51          2463            10            483 42          21           1014           11            531                                         Additionally, 2 "clumps" of translocator-43          19           918           22            1063                                         sized veligers were found on one slide-44          21           1014          25            1208                                         one "clump" of 14 (72-145um length) and 45          16           773           2.5            121                                         another of 31 (72-t70um length).
46          39          1884             2             97 47          37          1787 48          35          1691 49          20           966 50          22          1063 Avg.                      968                         595 Min.
Max.                    4492                         2705 2
Dens. or/            8960                              3 Analyzed by        Dite             Reviewed by                  Date

D. C. COOK PLANT ZEBRA MUSSEL MONITORING PROGRAM WORKSHEET Mexel Test - Art. Sub. Size and Density Calculation Sheet Date:          11/9/06 Sample    Mexel - Untreated           Mexel - Treated              Mexel - Untreated                           Mexel - Treated Veliert Mmboneter      Size        MirMa              Size                    # of veligers                                 # of veligers Number      Reading       (u)         Reading            (u)                       per slide                                    per slide 1         45        2174            18              669          Slide #                                   Slide #
2          8         386            16              773              1            72                           1               36 3          7         338             9              435              2            87                           2               39 4           7         338             5              242              3            55                           3               116      A 5          8         386            19              918              4            69                           4               48 6           5         242             6              290              5            93                           5               32 7           6         290            15              725              6                                         6 8           7         338             6              290              7                                         7 9           7         338             7              338                                                        8 10          6         290             8              386              9                                         9 1932             10                                        10 1          9         435            40 12         36        1739            12              580           Avg.                                      Avg.
                                                                    # /slide        75.2                      # I slide           54.2 13          9         435             8              386 14          5         242            25             1208 Is           6         290            10              483 16          10         483            10             483                                                         A 17          15         725             8              386                                   2 "clumps" of translocator-sized veligers 18          10         483            18              869                                   were found on one slide- one "clump" of 19          11         531            15              725                                   45 (97-170um length) and another of 12 20           8         386            14              676                                             (97-145um length).
5         242            15              725 22           7         338            17              821 23           6         290            26             1256 24           5         242            25             1208                                                    Mexel - Treated 25           4         193            18             869                                                        !settled organisms) 26           7         338            10             483                                                                    # of veingers 27          16         773            23             1111                                                                     per side 28          11         531            11              531                                                    Slide #
29           5         242            14             676                                                         1                35 30          10         483             6             290                                                         2                39 31           7         338           21             '1014                                                        3                59 32           6         290            11             531                                                         4                48 33           7         338           23              1111                                                        5                32 34           7         338            16             773                                                         6 35           6         290             9             435                                                         7 36           6         290           44             2125                                                         a 37           6         290             1              48                                                         9 38           6        290             15             725                                                        10 39           5        242              9             435                                                      Avg.
40          48        2318            11             531                                                    # I slide           42.8 41          33        1594            12             580 42          19        918              8             386                                                 Density            2282(,6667 43          6         290            20              966 44          6         290             12             580 45          7         338             12             580 48          7         338             16             773 47          6         290             6              290 48           5        242             10             483 49          5         242             10             483 50          8         290             7              338 Avg.                   488                            683 Min.                   163                             48 Max.                  2318                          2125 Dens. _40,107                                     28,907 E. Scott Rose /<w l 11-9-06 Aolzdb_          aeReiwdb       Re-viewed by Analyzed bf"         Date                                             Date

D.C. COOK PLANT ZEBRA MUSSEL MONITORING PROGRAM WORKSHEET Mexel Test - Art. Sub. Size and Density Calculation Sheet Date:    12/7/06 Sample  Mexel - Untreated           Mexel - Treated               Mexel-Untreated                          Mexel-Treated Veliger Micieornet      Size       Nmaer              Size                     # vellgers                            # ve.igers Number    Reading         (u)        Roatn              (u)                      per slkis                             per alle 1      25         980            14              549         Slide #                                 Slide #
2      22         862            10              392             1            75                         1         134 3       9          353           20              784             2           214                         2          94 4      20          784            9              353             3           202                         3         114 5       8          314           31             1215             4           228                         4         141 6      25         980            15              5s8             5            82                              5     87 7      22         862            29             1137             6                                       6 8      63         2470           30             1176             7                                       7 9      13          510          27              108              8                                       8 10      14          549           15              588             9                                       9
       ..      13          510           13              10             10                                      10 12      10         392            18             706            Avg.                                   Avg.
13      28         1098           16             627         # I slide       160.2                    #Islide       114 14      83        3254            16             627
 --            81        3175                           341 18      56        2195             9             353                                                       There was, for the first time, no evidenco of 17      13         510           43             1886                                                        "clumping" on the Moxol-treated slldes.
18       7         274           37              1450 19       8         314            16             627 20        7         274             7             274 21        7         274           2              1058 22       26        1019           29             1137 23       31        1215            9              353 24       310                                      392 25       21         823            12             470 Mexel Carbon Steel Samples 26        8         314            10             392          One Test and one control carbon steal coupons were removed from 27       14         549            10             392        the treatment system and analyzed. The coupons are 1/2" wide X-r 28        8         314           13              510                                              2 long. 3 Q 1" sections (1.50 In total ares) were viewed under the 29       19         745           13              510             scope. Settled   vellgers  on these 3 sections were counted and
..... W0                  353            12             470-                                           measured 31        a         314            6              235                                             Untreated Coupon                     Treated Coupon 32        7         274           22              862 33        7         274           15              588                                    Section I Section2         ction3 Section I Section2Secion 3 34      57         2234           12              470             # post-vligers              90          87         134         40            14        is 35        9         353           17              566                                                    918         242       2174          103       1014 36        8         314           17              666                                      2,483        1352         338        1014         1159       435 37      35         1372           15              588                                        878        1159         290        725           821       580 38      12          470           14              549                                      1,258         889         531       1258          1846      1401 39      14          549           14              549       Randomly chosen po             1            1654         628        531          1063       531 40      27         1058           10              392               veoiger Sze             918          580         242        580           918       58 measurements(urn) 41      60         2352           80             3136                                      1,014        1014        1,787       483           678      1449 42       8          314           37             1450                                      1,304         725         531        580           89        986 43       6          235           30             1176                                      2,029        1083 11,401            1111           531      1739 44      12         470            24              941                                       483          918         388        988           725       725 45      19          745            8              314            Avg. Size (urn)           1,492        1,189        638        942           937       942 48       8          235           27             1058 47       9          353           57             2234         ,Density,(VW)               27,000       260,700     416.400    124,000       43,400    48,500 48      15         588             9              353          Avg denslty (UW/)                      321,367                             71,300 49       6         235            15              588 50       8         314            16              627                                    Densities based on 1550trn/M" Avg.                1073                           749 Min.                 24                            23 Max.                 3254                          3136 Dens.               85440                    L      8                1000 /&#xfd; E.Scott Rosed b       41 12-708           Reviw                                           6b Analyzed bf -        Date             Reviewed by                    Date

Date: 6-7-2007 2007 k'exel Control GO,!T PROL                                       TREATED                                        Densitv (ml Sample     2007 Nexef               200Q6     (Wre),        2007 Mexel               2006 Mexel                Subsample        veliQers/mO Veliger                                        _______l                _____         _________              I___               ______
Micrometer     Size     Micrometer      S"zo    Micrometer       Size    Micrometer       Size Number     Reading         (u)     Reading         (u)     Reading          (U)     Reading          (u)             1                 11 1       2.5         121         20           966         2             97        38           1835              2                22                   20.0 2         7          338         30           1449        3            145        73           3526              3                 7 3         2           97         39           1884        2             97        28           1352              4                 15 4        2.5         121         35           1691        4            193        30           1449              5                45 5         2           97        112       1 5410 1        2             97   1    44      1 2125                 6
       -   e            I    -~   t     -.          .~. I     -      I     ~. I     -.      I            e                 1              I 2037 Wrax~el G~trol t        1.5          72    j    54       1 2608    1     3            14U5          4    1    4057              7" 7         4          193         30           1449        4            193        28           1352              8                            10er,36y
(#1ma3) 8         2           97         59          2850        31           1497        53           2560              9 72         41      1    1980        2             97        37           17871            10 10         2           97         30          1449         2            97         58           2801            &#xfd;Uua Iwetel '07-777
.. T.i         2           97        342         16519        10           483         31           1497                Drnsil   i#
12         2           97         44          2125        2.5           121        25           1208 13         3          145         25          1208         2            97         28           1352        Subsample 14         3          145         30          1449        1.5           72         63           3043              1                166 15         3          145         72          3478       2.5            121        30           1449              2               227                 204.3 16         2          97          31          1497       2.5            121        13            628              3               220 17       2.5          121         52          2512        3.5           169        70           3381              4 18         3          145         40          1932         2            97         52           2512              5 19         3          145         31          1497         2            97         61           2946              6 20        2.5         121          97          4685         2            97         17            821              7                        2CO06 IC            O 2           3         145          87          4202         3            145        55           2657              8 22          3         145          30          1449         2            97         40           1932              9 23        3.5         169          50          2415         2            97         22           1063             10 24          2          97          74          3574         3            145        35           1691 25          2          97          39          1884         2            97         21           1014           2007 Mexel Treated 26          2          97          55          2657         2            97         17            821               Densiy (#1m 2) 27        2.5         121          28          1352         3           145         77           3719                              # of 28          2          97         40           1932         2            97         31           1497       Subsample         vellgers/ml 29         2           97          21          1014         2            97         11            531              1                23 30          3         145         25           1208         2            97         43           2077              2                14 31         4          193                                   2            97                                        3                14            13.0 32        2.5         121                                   2            97                                        4                 6 33         3          145                                 2.5           121                                        5                 8 3          145                                   3           145                                        6 35         3          145                                   2            97                                        7                             603exel Treated 36         5          242                                   4      1    193   j                _         1         8_  _
37         2           97                                   2      1     97                                        9 38         2           97                                   2            97                                       10                             63,933       1 39         3          145                                   2            97                                     2006 Mexal Treated 40       2.5          121                                   2            97                                         Densil (#lm2) 41         2           97                                   2            97                                                        # of 42         4          193                                   2            97                                 Subsample velgers/ml 43         4          193                                   2            97                                        1                78 44         2           97                  1                2            97                              1        2                 64 45         2           97                                                                                         3                 79                   73.7 46         2           97                                                                                         4 47         2           97 48                                                                                                       1        6:&#xfd; 5             -
49                                                                                                                7                         2006 Mexal Treated 50                                                                                                                8 9
Avg.                   129                     2677                      154                     1956 10
________       I 39,289 Min.                   72                       966                     -T272                     531 I*j:Io                                            AA7 Dens.             (                              REF                   #REFI    1#E                      I
                                                                                                                          .__._7k

Date:        8/23/07 2007 Merel Control Sample
[EXEL-CONTROL                                  I    MEXEL-TREATED                                           Density (#fm )
2
                                                                                                                                                #o Valiaer         2007      terel          2003 lexel                2007 Mexel             2006 Mexel                Subaample            velliher Pconw          Size      hU*Ju            Size       Mkrorew     Size        b               Size Number        Reading         (u)      Reding           (u)       Reading      (u)       Reading            (U)             1 1                                      5          267                                   4            214              2 2                                      8          427                                 163          8704               3 3                                     5           267                                   8           427               4 4                                      4          214                                  17           908               5 5__3 1             1                   5_1        320                                  10            534              6                DIV/O!      Avg 6                                    17     1     908                                  12    1 641                    7                            2007 tvre),a Control Control 7         121 11211_                                     7           374               8      1                        Density) a8                                  280       114952 1                17                            374               9         1                      (#!rn3) 9                                   157     1 8384                                     15    1      801             10                                #D!VIO!]
           *1 -1*                     t     --   ,~       - -                    t         -   1*      --     9      ,...       -   ~              I-.
1U                                    It     1 4UM                                      l1    i      tU1                              L-Oillzrc-l 11                                      5          267                                 1140         7476                 Density (#I/m 2) 12                                     7           374                                  112          641                                  Sof 13                                     4           214                                  10           534        Subsample            veratsslde 14                                      8          427                                  10           534               1                 1204 3                                     fT          427               2                 872             1038.0 Avg./slide 16                                     8           320                                  10           534,              3                                 Due to heavy and mature 17                                     a__         320                                  41          2189               4                              settlment only 2 lidn were 18                                     9           481                                  10           534               5                               analyzed, Visually the moat-19                                   127          6782 1-0840 13 12 694 641 s
7
__ and the    l_ pulated 20                            23
                    -0          -FV                                                                                          7                            200 14exel Control 21                                                                                      1-T          MM_ .                                              Density 22                                                                                       11           587               9         1                      (#lm3) 23                         --                                                            10        '534               10            _53,600 25                                                                                        8           427          2007 Mexol Treated 26                        -                                                              13               92 27                                                                                        9           481                                  # Of           #
28                                                                                 .... 10            534        Subsample            veIld8           -100um 29                                                                                        6           320               1 3a                                                                                        8           427               2 31                                                                                       18           961               3 32                                                                                       12           641              4 33                                                                                        10           534               5
_4                                                             10           534              8                #DIVI0!      Avg.
35                                                                                       27          1442              7                             2007 Mexel Treated 36                                                                                        12           641              8                               Density 37                                                                                       118          6301              9                                 (#Im3) 38                                                                                         9           481             10                                #DIV/01 39                                              _                                         11           587          2008 Mexal Treated
                               -40                                                            9            481                 Density (#/m2 )
41                                                                                        14           748                                  $of 42                                                                                        19          1015       Subsample             ve*i4W&rde 43                                                                                       34           1816               1                 324 44                                                                                       183          8704              2                  338 45                                                                                        17           908              3                  174               247.4 Avg./slide 46                                                                                        6            320              4                  198 47 '_14                                                                                                748              5                  203 48                                                                                       13            694              6                           1 49                                                                                       12            6411             7         1                  AVVQ    ID 4           I            +              4           4        4             4              .4                  I                            1~
50                                                                                       46           2456              8         i                  nrn-itw 9                                 3#4m3)
~4~I~                               b                                                C                -4                              4 Avg.                                               2561                                               13831             t                                131,947
                                                                                                      -            a-                 I Min.                                                                                                   214 Max.                                                14952                                              OQAA Dons.                                              #FF                      WlVTOT                 -i
-          C-           &-           I ~1.          -           a.-         &~       &~            J. -
                                                                                                                                                                        ,Date          7I-Analyzed by                            Z -' aTwe                      Analyzed  by            DaleReviewed by Reviewed        by                        Date Clio

THIS" PAGE INTENTIONALLY LEFT BLANK

Analysis of Mexel Test Blo-box Baffles           U                                            U.......
Date:      9/6/07 2007 Ittexel Control
['EXEL-CONTROL                                      MEXEL-TREATED                                     Density (91I)
Sample                                                                                                                            # of vewigewr Veliger                                                                                                        Subsample 1 in2 scrping MWirometer       Size                              Micrmete        Size Number         Reading           (u)                              Reading         (u)                                  1                16 1             5            167                                   4          193                                  2                 29 2             8           266                                    8          386 W                                  3 3            19           633                                    4          193   1                              4 4             7           233                                    6         290                                   5 5_           12           400_                                   5.[~      242~     __                           6_              22w          JA___
6           22            733                                    7          338                                  7                             Z007 Mclixel Control 7            13           433                                    5         2421                                  a                              Dansity 8            14           456                                    8         3861                                  9 9            12           400                                                                                    10                        (    34,875      .
       -    1      ~,.          ...... I             I       I            1          t        1-              -               -
10            10           333LI I- -~         +   ~          I             I                    4
* 4-11           304         1483 12           52          2512 13           11            531 14            5            242 15            7            338 16           68          32842 17 18            9 10          435 483         ___
2 @ 1 in scrapings 19             0          4290                      were taken from each 2T             9 __        435                          baffle - one from a 2122     _-sparsely                                                     populated 23                                                       area, one from a 24-25                                                     -densely populated                                        2007 Meeil      Treated 2T                                                                   area.                                           Density (#/m 2) 27                                                                                                                             # of vellgers/
28                                                                                                          Subsample I1n2 =raping 29                                                                                                                 1                  7 3021 31                                                                                                                 3 32                                                                                                                 4
___                                                      _                      I                                      5 34     L              I              &               ~                  I          J        I           &-..--...&
4.0 35                                                                                                                 7                             20c )7 Mexel Treated 36                                                                                                                 8 _                              ensity 37                                                                                                                 9                                    3 38                                                                                                                 10                         (    6 I.            +        4                       4        I 39 40 41 42 43 44 45 46 47 48 49 50                            48   _____
Avg.                       1365                                               2864 Min.                        167                                                                           I--
Max.                       14683                                              386 Dens.                      _9:9                                                                   -f#
bj"&#xfd; Piw E. Scott Rose/$Sd                 / 9-6-07                                                                                       , ?T/o7 Analyzed 09-"                               Date                                           Reviewed by                            Date

APPENDIX 4 FINAL REPORT Mixing Zone Eval'ation for the Donald C. Cook Nuclear Plant Discharge Plume in Lake Michigan Prepared for.
AEP Cook Nudear Plant One Cook Place Bid man, MI49106 Prepaied by:
Gre    La*s E         ,rk).nsntIe-Great Lakes EnvAronmental*Center Traverse Cty, MI 49686 Phone: 231-941-2230 Facsimile:- 231-941-2240 Principal Contact Persons:
Dennis ..McCauley dmccaulevy@giec*com Doug Endicott.
dendieOtt@,gecjconm April 20,2:006 iiq

AEP' Cook Nuclear Plant fixing Zone Evaluation                                                       April.20, 2006 EXECUTIVE 

==SUMMARY==

The Indiana Michigan Power Company's Donald Q. Gook Nuclear Plant located onthe' southeastern shore of Lake Michigan is seeking to modifyi-ts NPDES Permit toalloW the use, of the proprietary molluscicide, Mexel 432, to'control the settlement and, growth of zebra mussels and quagga mussels 0on..the:intake tunnels of the circulating water sysem.
The Michigan Department. of Environmental Quality has calculated a waterquality criterion for Mexel. If this criterion is applied to the Cook Nuclear Plant as an end-of-pipe limit, the limit will be exceeded. The objective of the mixing zone evaluation,was to summarize the existing data in: a .reprtto .theMichigan Department of Environmental Quality (M4DEQ) to determine whether a mixingzone is acceptable and protective of the designated uses andiwaterquality of the receiving water (Lake Michigan). Ultimately; the, goal of the dernonstration is to achieve compliance for future. Cook Nuclear NPDES discharges with Rule 51 of the Michigan Wafer Quality Standards, specificalIly, Rule 323.1082 (Rule, 82, Mixing zones); Sub-rule 7.
The State of Michigan water quality standard alloWs dischargers to meet waterquality criteria at the edge of amixing, zone. Michigan's regulation defines mixing zone as, "'that portion of a water body allocated by the department'where a point sourcedor venting jgroundwater discharge is mixed with the surface waters of the state."(Water Quality.
Standards Partf4, R 323.1082(j1)) IfidianaMichigan Power Company was asked by the,;
MDEQ.to-determine the dilution ratio of the Mexel discharge concentration with Lake Michigan water. Michigan Surface: Water Quality Standards rule defines the' edge of the mixing zone as theqpoint where discharge nduced mixing ceases to .occur.
A computitional fluid dynamics model (FLUENT v6.2)-was used to determine the dilution. ratio :of Mexel in the :discharge from Cook Nuclear Plant, at, the edge of a miixing Zone, using Michigan water quality standards definitions and procedures.
Theimodeling results demonstfated thatf the dilution factor at theedge of the near-field
:mixing;zone'wi!! be approximately: 3.0 at the,2 ft./sec. (fs) isopleth. 'The modeling results also demonstataedd that thetwo cooling water discharges, do not overlap and that
'tle area:of the near-field mixing zone! foreach outfall'is relatively smal! And contained within seve'ral hundred square feet.
A review of theypotential impact on designated uses of Lake Michigan Water, concluded' that there was- no impact on Any designated use., Of particular concern, will be the impact of the application.of a molluscide Mexel A-432 to. the cooling water discharge' on Gteat Lakes fisheries: and'aquatic life. Cook Nuclear had previously developed a Tier Iwater quality criterion of 0.1 ing/ (100 ptg/L) for Mexel. No other water quality criterion is of concern at:this:time. The expe6cted'.maximum concentration of Mexei A-432 at the edge of the near-field mixing zone, with one unit treated at a time: is approximately 0.1 mg/L.
 ,The expected maximum concentration of MexelA-432 at'the edge of'the near-field mixing zone, with .two units treated &#xfd;s'imultaneously is approximately 0.2 mg/L.
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AEP Cook. Nuclear Plant Mixing ZoneEvaluation                                                                                               April 20, 2006 TABLE OF CONTENTS EXECUTIVE SUMMAR,              ................                     .............
Introduction .... ......   ..............       . . . ...........................................           ........................... .
Desciiption of theStudy Area and Intake and Discharge Configuration ........................                                                  2 Lake Bathymetry and Water Currents ............................... . ............... ,...2 Intake Configuration: Discharge Configuration ......................                                        . . .. ....2.
Discharge Configuration ...............                                     .......................                                     3 Review of Previbus M ixing Zone'Studies                         .....            ..- ............................................             4 M odeling Objectives...... ......................................................                 ....................                        5 Mixing Zone Definition ..................................... .....,,.,                                    .       .                     5 FLUENT Model .....................................................                                                                      6
      'Model Boundary Conditions ............                              ,..          .......... ................................        . 6 FLUENT Model Results.......;..........                                                        ......                      ...                6..
Impact on Designated Uses .............................................                                                                      i21 Great Lakes.Fishery, Aquatic Life and Wildlife .......................                                                                       2 Review of Water Quality Standards and Toxicity-Test Data ................                                                              13 PublicWWat aterr.........
Supply .................
S                                         ........ .S.......      . .... .        .............    ......................... 16 1.6

==SUMMARY==
ANT )CONCLUSIONS                           ......        ..................                                                          21 REFEREN CES ..............................................................                 ............                  .......
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AEP Cook Nuclear Plant Mixing.Zone Evaluation                                                     April 20,200k6 FIGURES Figure 1. Plan View of D.C. Cook Condenser Cooling'Water System Figure 2. Two fpslsopleth, Figure 3. Three. fjs Isopleth.
Figure 4.. Visualization of effluent. dilution within the discharge-inducedmixing, zone (plan view). FLUENT modeliprediction of ambient lake water fraction (i e.,
1/DR) on 2 f~s plume, surface velocity isopleth for zero ambient velocity, 2 discharge units: operating and treating simultaneously.
Figure 5'. Visualization of effluent dilution within.the dischargeqinduced mixing zone (plan view);.FLUENT model prediction of ambient-lake water fraction (i.e.,2.
ambient velocity, l/DR) on-3 f3ps plume surface velocity isopleth for fps discharge units operating and t-eating s.'imultaneously.
Figure 6. Visualization of stream paths for particles injected into the plumezat-the discharge point(s)
Figure 7.9 Map Indicating Location of Lake Township PublicWater. Stipply.hitake and CNP'Dischrge Structures in LakeMichiganr The distance betweenthese points'was measured as 3$,220, feet using survey methods and GPS controls TABLES Table 1. Predicted Average Dilution:Ratios (DRs)For Different .Abient Current Velocities, Plume Boundary Velocitiesi, and OperatingITreatment Conditions.
Table 2. Summary of the Designated Uses and the Impact of Cooling WaterDis'charge
            -onLake Michigan Offshore of the DC Cook Cooling Water 'Discharge.
Table 3. Summary of Acceptable @Mexel Toxicity TestiData (December 2004)
Table* 4. Mexel A-,432 Median Lethal Toxicant Concentrations (Lc50) Based on Daily Intermittent Exposures of 20Minutes EachDay APPENDIXk Appendix A. Current-Meter Data from NOAA/GLERL EEGLE Project.. Data Measured
                &#xfd;at Station: C4; Mooted'in 11 Meters of Water Offshore of the D.C. Cook Nuclear. Power.Plant.
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AEP Cook Nuclear Plant Mixing Zone Evaluation                                                        April ,20, 2006 Introduction The IndianaMichigan.Power Company's Donald C. Cook Nuclear Plant located on the southeastern shore of Lake Michigan is seeking to modify its NPDES Permit to allow the use of the proprietary molluscicide,,Mexel 432,.to control the settlement and groWth of
.zebra,mussels and quagga mussels on the intake tunnels of the circulating water system.
PlantfoperatorS. plan to inject Mexel into the circulating Water'system at the intake structures out in the lake. The Mexel would be circulated through the plant cooling system, and discharged back out int0 the lake through the cooling water: discharge:
structures.
The objective ofths mixing zoneevaluation is to smtimarize the existing data in a report to&#xfd; the.Michigan.Department of Environmental Quality MEQ) to determine whether a mixing zone is acceptable.,and protective ofthe,designated uses andwater quality of the receiving water (Lake Michigan). Ultimately, the goal of the demonsti-ation:is to achieve compliance. for, futureCook Nuclear NPDES discharges WIith Rule 51 of the Michigan Water Quality Standards, specifically, Rule. 323.1082 (Rule 82, Mixing zones);-
Sub-rule 7.
The MDEQ has calculated a water quality criterion1fr.Mexel. If this criterion is applied to the Cook Nuclear Plant as an end-ofrpipe limit, the limit will be exceeded-. For the&#xfd; treatments to: be effective, Mexel will need to be injected in. the intake at concentrations that will' not be degraded and diluted to a concentration less than or equal to the water quality criterion by the time the cooling water is discharged to Lake Michigan.In .other words, thedosage of Mexel 432.required to control zebra and quagga musseis will result in the discharge of cooling, water to Lake Michigan that exceeds, the water quality, criterion.
The State of Michigan water quality standdard allows dischargers to meet water quality criteria~at the-edge.of a mixing zone. Michigan's regulation defines mixinig zone as,, that portiOn of a Water body allocated by the department where a point. source or venting groundwateir discharge is mixed with the surface. waters of the state."' (Water Quality, Standards Part 4, R 323. 1082(1)) Indi-ana Michigan Power Company was asked by the MIDEQ to determine the dilutiontratio of the Mexel discharge concentrationwith Laked Michigan water. Michigan Surface Water Quality Standards rule defines&#xfd; the edge: of the mixing zone as the point where discharge-induced mixing ceases to occur. According to General Rule, Part 4 R 323.1043 Definitions;. A to L:
           "Discharge-induced mixing" means the mixing ofa discharge and receiving, water that occurs due to dischar'ge momentum and buoyancy up to the point where mixing is controlled by ambient turbulence."
A computational, fluid dynamics, model (FLUENT v6.2) was used to determine the.
dilution ratio of Mexel in the discharge ffromi CookNuclear Plant at the edge of a mixing zone, using-Michigan water quality.standards definitions and proceduires. The dilution ratiowas applied to the,expected miaximum end of pipe concentration of Mexel A-432 :to determine; the' expected nmaximum ctonc entration .of.Mexel A-432 in LakeMichigan inder.
I

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                         April.20, 2006 varying operational.scenarios. That concentration was compared to the calculated Michigan Tier I water quality criterion for Mexel A-432.
Description of the Study Area and Intake and Discharge Configuration Lake Bathymetry and Water Currents The bottom of Lake Michigan off shore of the Cook Nuclear Plant is fairly smooth and featureless. The bottom slopes gradually' ata uniformn angle from the shoreline out to .a depth of 50 feet at approximately one mile off shore. At that point, the slope of thedecent decreases and the,depth increases only 10. feet, from 5.0 feet to, 60 feet, over the next half-
.mile off shore. From there the, slopebecomes shallower and the depth increases only 15
,feet, from 60 to 75 feet, over the next two miles off shore.
The major surface water currents in the southern basin of Lake Michigan are generally in a counterclockwise directionq, giving the prevailing current past the.Cook Nuclear Plant a south: to north direction. North to sbuth currents occurs ihfrequently depending upon the wind pattern. Acoustic currentmeter data ifrm the.National Oceanic, and Atmospheric.
Administration (NOQ AA)/Great Lakes EnvironmentalResearch Laboratory(GLERL)
Episodic Events in the Great Lakes:Experiment. (EEGLE) Project was acquired. to characterize current velocities in the vicinity of the plant outfall structures. Water veloibtiesmeasured, in the fall of 1998, at.Station C4, moored in 11 rmeters of water offshore of the power;plfant outfalls', are presented as an appdendix to,this report. Positive u-components .ofvelocity (the second line 0on the data graphs, counting from the'top) correspond to south-to-north longshore currents. Examination of this time series, shows that current velocities are usually smaller than I0-20 cm/s (0.3-0.6 fps). Current velocities exceeded 40 cm/s (1.3 fps) twice during this period; these high velocities persisted for several-hours to aboutbone day. Given that the November-Jaanuary time period is particularly energetic in terms of wind, waves, and currents in the Great Lakes; ambient current velocities 'near the&#xfd; power plant outfalls will tend to. be smallerjin other seasons.-
Intake Configurationf The design intake flow'is 1,645,0Q0 gallons per minute, (gpm)for the condenser cooling water flow, 16,000 gpm for the essential service water, andg9000 gpm for the nonessential -service.water system,: forua total 'intake of approximately
                                                                          ,1 .67 million7 gallons per minudte. All cooling waterand service waterni's drawn into the plant throughmthree intakeltunnel&#xfd;'sthat extend about 2;250 feet-offshore.,Each tunnel begins With.an octaggnal-shape*d steel structure and velocity cap crib that protects the upturned elbow that is connected to the intake tunnel: Each intake tunnel is 16 feeft in diametetratd the tunnel carries the water from theIoffshore locationinto the screen house. The intake cribs ate located in 24 feet of water at 579 ft MSL water.elevation; Water flowsjinto the cribs through an 8 x 8.inch mesh grid work that is iritended to keep 'large objects out of the
 'intakes. The water velocity throug!.h the 8 x 8-7ii. grid is 1.27 fps and the watet* velocity through the tunnels is about 6 f*s.
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AEP Cook Nuclear Plant Mixing Zone Evaluation                                                              April 20, 2006 Each intake tunnel is 16 feet in diameter and the tunntel carries thewater from the offshore location into the screen house. Inside the screenhouse the Water enters a common forebay (conmon to both units). The water passes through steel trash racks composed of two designs. The original trash racks are composed' of 3 8-in thick by .4-in deep-bars on 3-in centers, giving an. openinig of 2 5/8-in. These       are being replaced over time with trash racks made ofbars set on edge to allow         a 3 3/ 16 -in clear Space between bars' (bars are 3 9 /16-in. on, center and the bar material is 3/8-in thick). From 'the trash racks, the water flows to pPtionally installed supplemental trash rack removable inserts placed in the traveling screen stop log slots directly in front of the traveling screens. These ifiserts. are made of 3/16 -in thick by2-in, deep horizontal bars spaced on 1 3/_16 in centers and vertical 3i16-in rods on 4-in centers leaving an effective rectangular clear space between tfhebars and rods of 1-in x 3 '3 /16-in. From there the water flows through the traveling water screens. The original screens were chain belt with 3 /8-in-mesIh:screens.
The original screens have been replaced with single entry single exit screens (with 3/8-in mesh and /i16-in. mesh screen material) manufactured'by Geiger International, Inc.
Di'scharge Configuration The cooling water is discharged back to, the lake through two tunnels buriedbeneath Lake
.Michigan.,The discharge structures are located 1,200:feet offshore:in 18 'feet.ofwater.
The total cooling water transit time from intake to: discharge is about ten minutes. The, Unit 1 dischatge tunnel. is 16 feet in didimeter and the Unit 2 tuinnel is 18'feet in diameter.
Both tunnels terminate with a 90&deg;:elbow that turns the watertflow from horizontal to vertical. The water enters the discharge structures'from the e.lbows and is passed horizontally through slots: inthe discharge structures. The Unit 1 discharge. structure has two slot openings, with an overall length of 27 ft. 10 1/8 in. and a height of 2 ft.,
ptoviding a cross-sectional area-of 11.36 ft'.2. At a cooling water flow, rate of 719,850 gpm (1603.94, ft.sec), thle discharge velocity from Unit&#xfd; 1 is&#xfd; 1-4.4 fps. The Unit 2 dischargestructure has three slotopenings, with an overall length of 19 ft: 7/8 in. and a.
heightf 2 ft. 9in,, provjiding a cross-Seciional-area of 157.33.ft.f,. At a cooling water flow rate of 950,150 gpm (2117.09 ft.sec), the discharge vel0city from Unit 2 is 13.5 fps. A conceptual diagram of the cooling water-system, including the intake and discharge structures, is provided in Figure 1.

AEP Cook Nuclear Plant-MiXing. Zone Evaluation                                                     April 20,.2006 Figure 1. Plan View of D.C. Cook Condenser Cooling Water System Review of Previous Mixing Zone Studies LTI conducted a modeling study of the thermal discharge from the D.C. Cook Nuclear PowerPlant in 2000 (Cook Plant!ThermalPlume Study; May 1,6,2000). The emphasis of:
that work was to simulate far-field characteristics of the discharge plume, Well beyontd the limits of discharge-induced. mixing.of interest here. H6wevedi, asp~art of the LTi study the CORM!X mixing, zone; model (Jirka;et al., 1996) was applied to capture the details of the strong mixing that occurs near the high velbcity discharge'structures.,- CORMIX was applied assuming both effluent discharge units were operating, and a long-shore ambient-current velocity of 0.03 m/s was used. The CORMIX'predictions indicatdddthat (1) the plumes from the. two discharge units did not: interact With each other (i.e., overlap) in the near-field, (2) the:thermal plumes would each reach thelake surface at a dista~e of4; 85
:meters from the respective diffuser structure, and (3) a dilution ratio of 2.2 would be achieved at this distarce. The, authors of the LTI report-did not' present theplume velocities predicted by CORMIX, so it is difficult t o relate:these results to the:mikihg zone defi.nition being used by the State of Michigan. However;,.the CORMIXimodel

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                       April 20, 2006 results can be compared qualitatively to the model predictions made for this mixing zone evaluation.
Modeling Objectives The object of the numeric modeling was to determine the dilution ratio at the edge of the mixing zone. Michigan Surface Water Quality Standards rule defines the edge of the mixing zone as the point where discharge induced mixing ceases to occur. Theoretically this definition of edge of the mixing zone is reasonable, however, in practice can be difficult to define. Ajet discharging into an ambient fluid entrains the ambient fluid. The entrainment is the result of a momentum exchange between the jet and the ambient fluid.
Near the source of the jet, the entrainment rate is high, the rate decreases as the jet penetrates the ambient fluid and the jet loses its momentum to the ambient fluid. When the momentum of the jet has been lost to the ambient, further mixing is the result of ambient turbulent mixing and diffusion. Ambient turbulence and diffusion causes mixing at the edge of the plume similar to jet induced mixing but at a much slower rate since there is no relative motion between the jet and the ambient fluid (Davis 1998). The transition from jet induced mixing to ambient mixing is gradual.
Mixing Zone Definition For the purpose of the DC Cook dilution modeling, the edge of the mixing zone is defined by considering the 3-dimensional velocity distribution for the discharge plumes, predicted by a computational fluid dynamics model. Isopleths (constant velocity surfaces) were constructed and visualized for velocities of 2, 3, 4, and 5 fps. For each iso-surface it was determined if a coherent jet structure was visible. For ambient lake currents of 2 fps it is reasonable to assume that a coherent jet structure is not visible on a 2 fps iso-surface (see Figure 2). Under the same conditions, an iso-surface of 3 fps clearly shows the jet structure (Figure 3). In each figure, the iso-surface has been colored by the inverse of the dilution ratio (i.e., l/DR). A 100 x 100 ft background grid is shown in each picture.
Selecting the appropriate jet surface velocity for defining the edge of discharge induced mixing was somewhat subjective. For this reason, results are provided for a range of velocities, Figure 2. Two fps Isopleth.                     Figure 3. Three fps lsopleth.
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AEP Cook Nuclear Plaint Mixing.Zone.EValuation                                                     April. 20, 2006 FLUENT Model The.commercially available software FLUENT was used for all the simulations.
FLUENT is, a, frilly three dimensional computational flliid dynamics (CFD) solving the Navier-Stokes equations on a boundary fitted mesh. A finite-volume&#xfd; formulation of the governing equations is solved in FLUENT. Turbulence closure was achieved using the RNG k-epsilon turbulence&#xfd; model (Yakht :and Orszag, 1,986). The energy',equation'was solved in the simulation to account for the. difference in the plume temperature and the ambient-temperature.
Model Boundary C onditions.
Three plant operating conditions were considered; Unit i1 discharge only, Unit 2 discharge only and discharge through Units 1 and 2. Each operating condition was si`ulated for four lake current-, conditions; a no current.condition, and currents of O.5, 1,.
and 2 fps. As illustrated by current.meter data (gee Appendix. A: lake bathymetry and water currents),' 2 fps is a relatively extreme high ambient'velocity. Thelake cUrrent was assumed to be fromrsouth to north and the nominal current is the depth averaged! value.
When units 1Iand 2 are in operation, the dilution ratio varies:considerably if both units are treated sitmultaneously or individually. Results are given for both conditions in Table&#xfd;
: 1. The unit 1 discharge inthe simulations'is 719,,850 gpm and unit,2 discharge is 9501,150 gpmn FLUENT Model Results Michigan DEQ,,surface water quality :standads rule defines the,;edge of the mixing zone&#xfd; as the:point-where discharge: induced mixing ceases to occur. For the purpose of this study, dilution ratios are reported on surfaces of constant velocity ("isopleths") ranging&
from 2'to: 55fps in 1 fps intervals. Avisuai1evaluation of the surface was used tolestimated if discharge indu'ced mixing occurred at a specific velocity. For ambientlake cuirents'of 0 to 0,5' fps, discharge induced mixing ceases-at a plume surface velocity of Ito 1.5- fps, depending upon the operating and' treatment conditions. For-an anmbientlakecurrent of 1 fps, dikcharge induced mixing ceases at a plume surface velocity of 1.5 to 3 fps,, while at, the highestambie`nt lake',current (2 fps)' discharge induced mixing ceases at a plume
,surface velocity of 3 fps.
Visualizations of effluent dilution predicted within the discharge-induced mixing zones are displayed in Figures 4 and 5. Both discharge units are operating in the simulations' shown in these figures. lI Figure 4, the ambient current velocityis O While, in Figure 5,,
the current. velocity is I fps., Cbmp1ison of FiguresA4 and 5 shows that increasing the, aambient velocity tends, to shrink the. extent of the discharge plumes, as well as the ehtrainment of lake,Water within the discharge-induced mixing, zone:. The yellow grid lines in *thevyisualizations are, spaced 160 feetzapar, to indicate the size-of the plumes.
The color scale shows the percentage of water from the discharge'. Warm colors (red-yellow) indicate less mixing with: lake water and cool colors (blue) indicate more mixing 6

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                 April 20,2006 with lake water. The discharge plumes from the two units do not overlap or interact within the discharge-induced mixing region.
Figure 4. Visualization of effluent dilution within the discharge-induced mixing zone (plan view). FLUENT model prediction of ambient lake water fraction (Le., 1/DR) on 2 fps plume surface velocity isopleth for zero ambient velocity, 2 discharge units operating and treating simultaneously.
7                                        \Ckf 11t

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                April 20,2006 Figure 5. Visualization of effluent dilution within the discharge-induced mixing zone (plan view). FLUENT model prediction of ambient lake water fraction (i.e., I/DR) on 3 fps plume surface velocity isopleth for I fps ambient velocity, 2 discharge units operating and treating simultaneously.
8

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                               April 20,,2006 Table 1. Predicted Average, Dilution Ratios (DRs) For Different Ambient current Velocities,, Plume Boundary Velocities, and OperatingTreatment
            .Conditions.
dischar eunitso eratin                   1 &2          1    11  2     1&          1     2 discharge units bein treated    W      t 1 2]Il=17.LIi.27LIL" 2'&
ambient current velocity (fps): 0 average DR-at 11fps jet vetlocity- I ...              47f 7.14         5.88 15.00' average DR at 1.5 fps jet ,velocity    I14.17 1LZ77I                         II1___!'...
averageDR at 2,fps jet Velocity                        Ii 3. 85,13.23    1 3.033.,323 average DR at 3 fpsjetveloCity            _2.,56    E1         3.13i2.63I          2.50 average DR at 4 fps jet velocity             22           2     .56    2.22        2.22 average DR at5 fps jet velocity7 2.00                  IL     2.22 20              2.00 ambient current velocity(fps): 0.5 average DR at, fps jet velocity        F7II........          !
LII714     4.00L aVerage DR at 1.5 fps jet veocit                            .13&#xfd; I!..         Iti average DR at 2fps jet velocitY             2.'38
                                                     !.388   3.03 2.70        2.86 average DR at 3 fps jet velocity          2.04 2.44 2.13            I1 2.27        2.08 average DR at 4-fps jet velocity I 1.85               2.17 1. 96       200      . 189 averagejDR at 5 fpsjetvelocity 1 1,69               111.96 1.79J 1.85               1.67 ambient current velocity, (fps): 1.0 Average DR ait .5 fps jet velocity, .,       1      EI47 ]I""       III:"III.'iII average DR at2 fps jet!veocity :                            J2.089IIi'!LI" iZ averageIRRat*3f s~eveiocit 1.59                      2.50 1.92 1      1.61         1.89 average DRat 4fs jetfvelocit-              1.47    i 2.l      1.82     1.47      1.72 average DR at- fps jet velocity             1.37    EIi.II     1.69     1.39      1.59
                              -Ambient. currCnt_,elocity.(fps):.. 2Q...
averageDR at.3 sfps etyelocity              172                                   1.22 21.85.1.6 avyerage DR at f4S jet elocity          1.64        .2        1.67    1.56          1.92 average DR       5 fps   etvelocit        1.56        2.117 1.5        1.49        1.72 At zero ambient (ake)velocity, all operating/treatment conditions achieve an average dilution factor of greater than 3 (firom 3.03 to 7.14) at the 2 fps velocity bouidary used to define the plume, limits for dischaige-induced mixing (Table 1)&#xfd; As ambient velocity is increased, the discharge plume shapes and volumes change in somewhat complex ways that also become rmore dependent on the operating and treatment. conditions. In addition,,
it becomes more difficult to-identify the, discharge-induced mixing boundary. Although average dilutionratios in the. plume geoneraly decrease (in some cases do. to 1.5 to 21.0) as ambient velocity increases, there are instances where the opposite is observed :in-the modeling results. For.example, when discharge unit 1.is being operated and treated, the maximum predicteddilutin atioincreases from           ft     4.17 to;7T 14 as the: ambient velocity is 9

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                        April 20, 2006 increased from zero to 0.5 Tfs, but then declines to 4.77 as the ambientvelocity is, further increased.to: 1 fps.
Since the ambient velojcity in LakeMichiganI is usually less than 0.3-0.6 ifs, we. believe that the model predictions based on an ambient velocity of 0 or 0.5 fps.are the most representative for mixing zone determinations. At these: ambient velocities, the 1, 1.5 or 2 fps (depending, on operating/treatment conditions),discharge plume isopleths can be used to define the discharge induced mixing zone. As indicated in Table. I, dilution.ratios are greater'than 3.0'for all operating, and treatmenit conditions modeled at zero ambient velocity. At an ambientfYelocity of 0.5 fps&#xfd; DRs were predicted to range from2.4 to7,1, depending on operating and treatment conditions. Based on these results, we are.
confident thait-a dilution,ratio of 3.0 will bemaintained Within the discharge-induced mixing zo-ne under most conditions. Conservatively,_a dilution.ratio0 of2.4 couild be selected. However, we believe that using a DR lower than 3.0 is inappropriately conservative because many other safety factors are built.into the mixing zone evaiuation (see review of Water Quality Standards section).
The model results can also be used to calculate the-maximum contact time for a drifting organismthat, enters the discharge plume, Figure 6 is a visualization of stream paths for particles injecteddinto-the plume at the discharge point(s). The coior of the stream paths reflects the time of travel as, theparticies move from theopoints of:discharge to the plume boundaries. As can be seen from this figure; the average contact time-of a particle (i.ex, a drifting organism)- inthe plume'is about- 1 minute, with a maximum contact time of about 2 '/2 minutes. The significance .of this visualization is the consideration of the potenitial contact &#xfd;timne for aquatic, species, exposed to 'the cooling water discharge within the neart--
field miding2zoneiand the cotresponding:water.quality criterion concentation.
                                                   .10,

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                April 20, 2006 Figure 6. Visualization of stream paths for particles injected into the plume at the discharge point(s) ko0 11 15

AEP Cook Nuclear Plant:
Mixing Zone Evaluation                                                       April 20,.2006 Impact on Designated Uses The impact-of the coolingvwater discharge on the designated'uses of southe Lake Michigan-was evaluated by comparing the observations&#xfd; and results of this study to"the seven designated uses of the water body. The designated uses of Lake Michigan, which.
we evaluated, were:
1I. Agriculture
: 12. Navigation
: 3. Industrial water supply
: 4. Public water supply-
: 5. Great:Lakes fisherY
: 6. Other indigenous :aquatic life and wildlife, and
: 7. Partial bodyvcontact. recreationi-Of the seven'designated uses- outlined for this study, the potential impact to the Great Lakes&fishery and other indigenous aquatic life and: wildlifemay be of greatest, concern in..
tiis dinstance. We determined that there was,no impacttto any designated use in Lake Michigan, diae to the cooling water discharge. A summary of each.use designation,, likely-impacts-and rafionale are outlined in* Table2,. Additional discussiont of the potential impact of the cooling water dischargeon Great lakes fisheries, aquatic life and Wildlife, and public water supply arediscussed-berow.
Great Lakes Fishery, Aquatic Life and Wildlife:
The cooling water discharge at the DC Cook Nuclear Plant is authorized by the State4of Michigah.via a National Pollutant Discharge Elimination Sys~tem (NPDES)j permit. The conditions of that permit tequiire.tiat Cook routinely. monitor the concentration of various water quality con.stituents and compare. those to established water quality based standards that are specificdlly designed, to protect aquatic life andwildlife .inthetGrate Lakes. The' DC Cook Nuclear, Plant is in complete compliance with their NPDES permit.
Consequently, it is reasonable to conclude that the State of Michigan, through the
:extenIsive NPDES monitofring, has' determined that'there, is no impact to the Great Lakes fishery, aquatic life and-wildlife&#xfd;.
Of particular concern, will &#xfd;be the impact of the applicatiogn of aimolluscide Mexei A-432 to-the, cooling Waterddischarge.. Cook Nuclear Plant is required by itheir NPDES permit to provide prior notification for the use of any water treatment chemical or change in discharge pursuant.to Cook Nuclear Plant's NPDES Permit No. MI0005827, PartI.,
Section A.6, Requestfor "'Discharge of Water Treatmnent Additives" and Part ,I,. Section C. 10 "Notification-of Change in Discharge"., Cook Nuclear Plant will be requesting the.
!approyal of an intermittent discharge resulting from a daily application of Mexel A-432 to 'the' three circulating water intake tunnels to prevent zebra mus~sel settlement.
12.
I

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                      April 20, 2006 Review of WaterQuality, Standards and Toxicity Test Data One principaI objective for the DC Cook Nuclear Plant Mixing zone Evaluiation, was to.
evaluate the mixingcof the cooling water discharge with Lake Michigan water in the context of the application of themolluscide Mexel A-432 to the cooling water to control zebra: mtissels. Cook Nuclear had previously developed a Tier I water qualitycriterion for MeXel. No other waterquality criterion is of concerm at this time.
We reviewed the water quality information that is :specific to Cook Nuclearto determine compliance with State Water Quality Standards,. including the toxicity requirerienits of R323.10517 and R323.1082 of the Michigan Water Quality Standards.
Cook'Nuclear Plant's (CNP) intention is to use'Mexel 432/0 :in an intermittent discharge resulting from adaily application of Mexel 432/0 as A-432 to the .threecirculating water intake tunnels to prevent zebra mussel settlement. Specifically, CNP's proposal is to treat forup to one 30-minute period pet day of discharge of A-432 at. adaily average concentration not to exceed the established Final AcuteNValue'(FAV) for Mexel A-432
,(0.1 mg/L), with no one sample exceeding a maximum concentration of 1.5 mg/L for each outfall (NPDES Outfalls 001 and/or 002) as measured at each outfall's nearshore sample point during the treatment period and'adjusted for the expected concenottration at the end'of theOpipe and mixing zone. CNP in collaboration with Mexel and Great Lakes Environmental Center developed a-Tier, I FAV for MexelA-432 followingtheeMichigan DEQ Rule 57 guidelines.
The aquatic toxicity test data generated by CNP and Mexel satisfies the MDEQRule 57
,requirements for a TierIFAV calculation (Table 3), and provides intermittent dosage aquatic toxicit test data that demonstrates he, reduced toxicity of Mexel A-432 when applied intermittently (Table 4). Table 3 lists the FAV as 0.092 mgiL, which was founded up to 0.1 rg/L for thepurposes of this evaluation.
CNP has used various biocideslov&r the yeadktsor shock treatmeits :to the intake tUrhels, These-treatments have prven' to be a:very efficient'means of removing zebra mussels.
An efficacy rate of greater than 95% has been realized by applying a biocide for 12 hours as a shock-treatment to the intake-tunnels, However, uncontrolled sloughage ofslhell debris creates a heavy load on the traveling screens and pump strainers downstream from theL intake tunnels. The sloughage of shells could: possibly overwheim:and block flow in-the safety systems required by the NRC at all times for safe operation. In addition,6 biocides previously used require detoxification with bentonite clay..This process is: a potential source of silt intrusion that may clog vital. heat exchangers required for safe shutdown of thequnits.
The CNP proposal to use adaily 30-minute treatment:of A-432, targeted at the zebra mussel post-veliger stage will eliminate the uncontrolled release: of adult, shell debris. that potentially affects! the safe operation of the plant. A-432 would be applied simultaneously to the tunnels each day during the seasons when zebra in 1ssel     veiigers and post-veligers are the.most abundant (April through Novemberi) to. remve existing, iIussel Colonies and to prevent further settlement. Mexel A-43'2 is an aqueous dispe.sion of lineax aliphatid 13

AEP Cook Nuclear Plant.
Mixing Zone Evaluation                                                      April 20, 2006 aminies..!t is in the general category'of filming Amines, differing from otherWater treatment products in that it treats the wetted surfaces of the system without having to treat the waterncolumn. Mexel A-4322 fuctibns a a corrosion inhibitor, dispiersant, and control agent-fot cooling system-fouling species such as mussels and hydroids; The recommended dosage is 4,ppm for 30 minutes per daytVo strive. for an effective.
concentration- in the tunnel. Our calculations.for determ.ining.effluent:con.centrations:aret; outlined below.. When all three tunnels are.dosed at one time; the injected-concentration of 4 ppn Will be decreased by 1) the demand factor of 0.38 at the tunnel inlet, 2) by-a mixing-zone! factorof3.0, and 3) by a 0.38.demand factorinthe mixingx .zone. -This treatment will result:in-an expected maximum efflieni conce.ntration of 0.51 ppm d~uring the 30 minute treatment-period in-the effluent, (4.ppm x 0:62 x 0.62/3.0).
When one tunnel is dosed at one time, the effluent concentration will depend upon which tunnel is dosed, because baffles in the plant intake forebay prevent. complete mixing between. lake water drawn through, the&#xfd; three intake tunnelIs. The average concentration reductions -ineach tunnel, based upon measurements (Mallen, 2004),. are 9, 61 ,and 15%
for the, north, center. and south tunnels, respectively. So-for Mexel injected into the north intake tunnel, the injeqted concehtration:of 4 ppm.will be decreased by1) a demand factor, of 0.38 at the tunnel inlet; 2) a .concentration reduction of 9%/due to forebay dilution, and 3)'a demand factor of 38% in the-forebay. Theqmixing zone dilution ratio is 3.0, andt1hereis another 38% demand factor~inthe mixing-zone! For this case, the mixing zone con'centration:is calculated to be 0.29-ppm [4 ppm x (1-038) x (1-0.09).x. (l-038)-x-(1-0.38)/3.0%=0.29ppm]*. For injection into the centerintake, tunnel, the mixing zone coricentrati6n-is calculated ito be 0.12 ppm [4ppm x (1-0.38) x (1-0.61)-:x (1-0.38).x (I-Q.38)/13i.0 = .1-2 ppm] And, for injection into thesoiith intake tunnel, the mixinig zone concentrationis, calculated t6'be. 0.27 ppm [4-ppm x (1-0.38) x (1-0.5) x (!O.38) x (1N 0.38)/3.0 =0: 12:ppm]., Once CNP begins dosing, they-will be able to corroborate these projections?,by actual measurement. Measured demands at other 1o6cation~s agreed with these:projections.
However, itjis important to ermphasize that-this is a very conservative, estimate of the:
maximum expected, concentration durng :a.thirty-minute interi'al once; a day. The final concentration will be much lower because, 1'our degadation estimates are based solely on the water demand and. dilution, 2) the: demand calculation does.not include aIlowancess for surface adsorption:or for the demand due to biodegradation, 3) Mexel A-432 is a.
filming amine, part of the chemical concentration will.-be:lost due-to the formation-of the film, and 4):our calculations :also. exclude the demand at the edge ofthe mixing zone and inthe condenser water boxes within the plant due to turbulence. Consequently, we are confident that the actual measured maximum concentration will be much lower than our projections: Once CNP begins dosing,, they will be able to corroborate these projections by actual measurement. The final average-daily concentration. will be fardless than the FAY because of the daily intermittent application of the chemical (30.minutes). Mexel's experience with measured demands atoother plants has agreed.with the projectibs.ns, and.
We are confident that they will be able to. do the .same-at Cook.
14

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                         April 20, 2006 Consequently, the. final average daily coficentfation that Will enter Lake Michigan at the edge Of the demonstrated mixing zone as a result of this rep09Ttwil! be protective of aquatic life. Our basis for this is,that:
: 1) The maximum expected concentration of Mexel A-432 at the edge of the near-field mixing zone Will be equal to or less than the' calculated water quality criterion.
: 2) The expected contact time of a drifting organism potentially drawn into the discharge plume is less than two minutes, whereas the calculated water quaiity criterion is based on exposures measured in days.
: 3) Mexel A-4321rapidly biodegrades in waterj Its'half-life 'in still water is less than 22"hours, and the half-life,can be further reduced to six hours with agitationi and aeration.
4). Its toxicity to aquatic life-has been well demonstrated (See attached toxicity test information), and the proposed. intermittent use and .short duifation of the dosages further reduce the irmpact on the. environment. In fact, &#xfd;this application provides' data that demonstrates that the toxicity o9fMexei A-432 is significantly reduced when aquatic organisms are exposed to the chemical on an intermittent daily dosage pattern similar to the typical field application of the product.
: 5) The, degradation products of A-432 consist of Water; carbon dioxide; and nitrogen. Product that has not degraded or adhered to the walls' of the. cooling
             .system.will'be discharged with the cooling'wate~r from the plant.
CNP has:also de-veldped intermittent dosage toxicity test data for'Mexel A-4312 that:
dnemosttates. that the toxicity'of this substance, is .less durin!g intermittent exposures than with continuous&#xfd; exposures. That data demonstrates that the medianlethal concentration of Mexe A-432 applied as an'intermittent dose i's more than 44 times less than the demonstrated lcthal concentration in continuous exposures',(based.on aD..miagna GMAV of 0.197 rng/L and an intermittentdosage LC50 of 8.7 rg/L). This is an important. site-specific characteristic because even though we do not expect that the final.end of pipe concentration, will exceed the FAV,. MDEQ can be confident that the final discharge.
concentration will bemuch lower than:the known toxicity of this compound when it is appiied intermittently. Aquatic life toxicity:test data using fathead minnow, Daphnia ming6a' and rainboW trout in intermittent daily'd sage. experiments are surunarized:.i Table 4. The fathead minnow .andDaphniamagna intermittent toxicity test.data were:
generated by the Lake Superior .esea*rch Instituteat~ the University of Wisconsin-Superior and the rainbow trout intermittent dose toxicity test data was recently generated' at the Great Lakes. Environmental. Center in Traverse, City, Michigani.
Based on the&above donsideiration of the data, it is reasonable to* conclude that the application of Mexel A-432 to control zebra' mussels will have no impact on Great Lakes.
.fisheries; aquati& life or wildlife, 15

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                    April'20,2006 Public Water Supply The intake for the Lake Township public water supply (P WS)'is located 3,220 ft.
southwest of the CNP discharge structure in Lake Michigan (D.C. Cook Condition Report,* 1998). The PWS intake and CNP discharge structure are located on a map in.
Figure 7. As.noted iihTab1& 2, the PWS is, located.well beyond the study area..Fluent model predictions indicate. that the. naximum extent,(length) of the discharge plume is aboutI2,500' ft from the CNP discharge structures. Thus, under no condition is the cooling water discharge.plume predicted to reach thelocation of thePWS intake, In addition, Mexel does not bioaccumulate or otherwise pose a human health risk at the maximum!
concentration at the edge of the, mixing zone. Based on these considerations, it is reasonable to conclude that the application of Mexel A-432 to control zebra mussels Will have no impact on any public water supply:
16 c2P

AEP Cook Nuclear Plant, Mixing Zone Evaluation                                                  April 20, 2006 Table 2:. Summary of the Designated Uses and the Impact of Cooling Water Discharge on Lake/Michigan Offshore of theDC Cook Cooling Water Discharge Designated Use           Perceived.Impact (if any). Rationale; Agriculture:             None                       There is no evidence of irrigation water removal.
Navigation               None%                      The.CNP co-oling water discharge does not cause any obstructions to recreational navigation in Lakel Michigan. The.diffuser structure is. 18' feet below the surface.
Industrial Water         None                       There are no other industrial water Supply                                              intakes within the study area.
Public-Water Supply      Lake Townshippublic        This pubilic water supply is located water supply intake is     beyond the study area; model.
located 3,220 ft southwest predictions indicate that the-maximum.
of CNP discharge           extent.of the discharge plume is abouti structures inLake          2,500 ftfrom the CNP discharge Michiganqtructures..                    Mxel does not.         "
bioaccumulate or'pose a human health risk at the maximum concentration at the edge of the mixing zone.
Great Lakes Fishery      NoneI                      The expected maximum concentration for Mexel inILake Michigan at the edge of the mixing zone is similar to, the measured criferia for Mexel. ,"The most sensitive species used in the criteria cal.culatioqn are&#xfd; excludedfrom the  'edge of the mixing zonhe due to.
discharge   velocity. Expocted contact time within the mixin'plu me is, less than two minutes fordifting
                                                    ,organisms.
Other Aquatic Life. INone                       TheMexel expected  maximum in Lake          concentration, Michigan  at the andWildlife                                          for, edge of the mixing zone is similar to the measured. criteria for Mexel The cooling water is neither acutely or, chronically toxic to aquaticd.
organisms' The most sensitive species.
used in the criteria calculation are excluded from the mixing zone due to discharge velocity. EXpected contact time within the mixing plume is less than two minutes for drifting, organisms;.
RecreationalPartial       None                      :The water'quality of the-cooling water Body.. Contact                                       discharge would'not be detrimental to hh 'manhealth ........            I 1.7, at

I AEP Cook Nuclear Plant Mixing Zone Evaluation                                                    April 20,, 2006 Table;3.. Summary of Acceptable,@MexelToxicity Test.Data (December 2004)
      'Species            Investigator          LC5 o(mgIL)        GMAV                  FAV Bluegill Sunfish       GLEC, 2004!         -L71*'                                 _
Planaria,              GLEC, 2004           2.03 Hyalella azteca        GLEC, 2004            1.99_
C!iironomus            GLEC, 2004,          8.82 ten tans Rainbow Trout,         GLEC, 20,04          0.450 Brooke et al,;       0.730             0.5731*
1997 2 Lumbriculus            GLEC, 2004.           .1.86' Fathead minnow         GLEC&#xfd; 2004            0.450 Brooke detal,*        0.360 1997 Biooke et al,.        0.660.           0.4746*
                       '1997 Daphnianiagna           GLEC, 2004.          0.02001 Brooke etal,          0.0121
_1997 Brooke et.a.1,        0.216.
1997' Brooke etali,        0.199 1997 Brooke et al,         0 178, Btboke et, dl,. 0.i120 1997 Brooke et.,al,        0.1268 1997 Brooke et al,        0.1698
                        .1997            . .    .. .
Brooke etal,.       '0.1,98:
1997 Brooke et al,        0.595             0.197*
1997 N =8 (SMAV)                         I 0.092' mg/L Z       LC50s used&#xfd; inthe Final Acute Value (FAV), calculation.
Fathead minnow, D. magna and rainbow trout data completed by Brooke,, et al was identified as acceptable by MDEQ from'the Mexel toxicity data base.
Tests conducted by Great Lakes Enyironmental Center, 2004.
*2:     Brooke et al. 1:997.' Tests conducted by the Lake Stperior Research Institute.
18

AEP Cook Nucleark Plant MixingZone. Evaluation                                                  April 20, 2006 Table 4. Mexel A-432 MedianLethal ToxicantConcentrations (Lc50) Based on Daily Intermittent Exposures of 20 Minutes Each Day Species      Water Type        Daily Exposure      Test Duration          LCO0 Duration                               (mg/L)
(min. per 24 hrs)_
Fathead minnow       Lake Superior       20                 96                 6.2' (larval)             (USA)
(Pimephaes" promelas)_
Daphnia magna&#xfd;       LakeSuperior        20                 48                 8.7' (neonates).          (USA)
Rainbow Trout        Lake Michigan       20                 96                 3.22
( Onchorhynchus      (USA):
mykiss)________
1     GhMllebaert, F. and L.T. Brooke. 1997. Mexel 432,toxicity tocladorceran and fathead minnow during continuous and daily intermittent exposures. Lake Superior Research Institute, University of Wisconsin-Superior, Groupe. d'Embryotoxicologie des Poissons, Universite'Paris 7, 12pp.
2     Great Lakes Environmental Center, 2004, LC50 Determiniation for Mexel A-432 Using Rainbow Trout (Onchorhynchus inykiss). Final Report to RTKTechnologies, Inc*. Baton Rouge, LA. April 23,, 2004.
19:

AEP Cook Nuclear Plant Mixing Zone Evaluation                                             April 20, 2006 Figure 7. Map Indicating Location of Lake Township Public Water Supply Intake and CNP Discharge Structures in Lake Michigan. The distance between these points was -measured as 3,220 feet using survey methods and GPS controls.
20

AEP Cook Nuclear Plant Mixing, Zone Evaluation                                                       April 20&#xfd; 2006

==SUMMARY==
AND: CONCLUSIONS The AEP DC Cook Nuclear Plant conducted. a mixing zone evaluation to determine the dilution ratio of the plant cooling water with Lake Michigan water at varying velocities and distances. The mixing zone evaluation included a plime modelinitg study by Aldenr Laboratbries that provided a computational and visual basis for the mixing zone. The mixing zone evaluation also addressed the impact of the coolingwater discharge on the designated uses of Lake Michigan and reviewed Water quality standards, specifically the toxicity requirements of R323.1057. and R323.1082 of the Michigan Water Quality Standards..
The moddeling results demonstrated that the dilution factor at theedge of the near-field
:mixing.zone is approximately 3.. at the 2 fps isopleth. ConsetvatiVely; *the-dilution,factor would increase at ambient. currents less than or equal to 0.5 fps. At an ambient velbcity of 0.5 fps, DRs were predicted to range-from .2.4 to&7.1. The modeling results also demonstrated that the two cooling water discharges do not overlap and that -the area-of the nean-field mixing zone -for each outfall is relatively small and contained within several hundred squaree feet.
A review of the potential impact on designated uses of Lake Michigan Water concluded that there was no: impact on any designated use. Particular-attention was paid o the potential impact on Great Lakes: fifsheries, aquatic life and wildlife, and public water supplies. A review of Michigan water quality standards, specifically the toXicity requirements of R323.1057 and R323.1082 of the Michigan Water Quality Standtrds was
'completed, which also :supported the determination of no imipact.
Of particular concern, will be the, impact of the application of a molluscide Mexel A-432 to the. coolingwater discharge. One 6bj&ctiVe for the DC Cook Nuclear Plant Mixing Zone Evaluation was to evaluate the' mixing of the cooling water discharge With Lake Miichigan water in the context of the application ofthe molhuscide Mexel A-32 to the cooling water to control zebra mussels. Cook Nuclear provided sufficient data to the MDEQ :to develop a Tieri water quality criter ion for Mexel, No other water quality criterion is of concern at' this:'time. The calculated Tier I water quality. fiterion for Mexel A-432 is 0.092 mg/L (rounded up to 0.100 mg/L.or l0,Q .ig/Lfor this-evaluation), The expected maximum concentration of Mexel A-432 at the edge of the near-field mixing zone, with one unit treated at one, time is approximately 0:1 mg/L. The expected maximum concentration of Mexel A-432 at the edge of the near-field mixing zone, with two units treated at one time is approximately 0.5 !mg/L.
                                                 '21.

AEP Cook Nuclear Plant Mixing Zone Evaluation                                                       April 20, 2006 The assumptions used for the evaluation of the toxicity of M*xel A-432 within the neat&#xfd;-
field mixing zone are:
: 1. The tecommended ddsage, will be 4 ppm (mg/L) for-30 minutes perday to strive for an effective concentration in the tunnel.
: 2. When all.three: tunnels are dosed at one time, theinijected concentration of 4 pprii will be decreased by: 1) a demand factor of 0.38 at the tunnel inlet, 2) by the mixing zone factor of 3.0, and 3) and by a 0.38 demand.factor in the mixingR zone. This treatment will result,in an expected maximum effluent' concentration of 0.5-1 ppm during-the 30 minute treatment peri.d in the effluent [4,ppm ,x-(1-0*38) &#xfd; (1-0.38)/3.0, = 0.51 ppm].
: 3. When one tunnel is dosed at one time, the.effluent concentration will depend
              ,upon which tunnel is dosed, because baffles in the plant intake forebay prevent complete mixing between lake water drawn through the threedintdke tunnels. This is. discussed &#xfd;on Page 16 (Review of Water Quality Standards,
             'and Toxicity Test Data). The mixing zone concentrations are calculated to be 0.29 ppm, 0.12 ppm, and 0.12 ppm.for dosing ofthl-e north, center and south.intake'tunnels, respectively.
Based on the above consideration of'the data, it is reasonable to conclude that the, "proposed application of Mexel A-432 to control zebra mussels will. have noimpact on Great Lakes, fisheries, aquatic life or wildlife, or any other designated use of the Great Lakes.
22

,AEP Cook Nuclear Plant Mixing Zone Evaluation.                                                  Apr!il20,9 2006 REFERENCES Alden Research Laboratories. 2005. Cook Nuclear Plant Plume Modeling Study (DRAFT).
Davis, L.R., (1998). "Fundamentals of Environmental Discharge. Modeling'". CRC Press, pp. 352.
D.C. Cook Condition Report. 1998. Condition ReportfP-98-04943.
Ghillebaert,, F. and L.T. Brooke: 1997. Mexel 432 toxicity to cladorceran' and.fathead minnow during continuous and daily intermittent exposures,. Lake Superior Research:
Institute, University of Wisconsin-Superior, Groupe d'Embryotoxicologie des Poissons, Universite Paris 7: l2pp.
Great Lakes Environmental Center. 2004. LC50 DeterminationforMexel A-432 Using Rainbow Trout,(Onchorhynchusmykiss). Final Repor to RTK Technologies, Inc. Baton Rouge, LA. April 23, 2004.
Jirka, G. H., Doneker-, R.L,, and S.W. Hin-ton, 1996. "User's Manual for CORMIX: A Hydro-Dynanic Mixing,Zone Model and Decision Support System for Pollutant Discharges ifnto Surface Waters", EPA#: 823/B-97-006.
LTI. 200. Cook Plant Thermal Plume Study; May 16, 2009 Mallen, E. 2004. Mussel Monitoring and Control Program. Assessment Number: sA-2003-REA-003-QH. Assessment Dates;*12/15103 to 01/25/04. Condition Report: CR-,
:033440131.
Michigan Department of Environmental Quality. 1999. Michigan Water Quality Standards. Part 31 of the Natural Resources and Environmental Protection Act, 1994 PA 451, as Arended.
Yakhot, V., Orszag, S.A., (1986): :"RenormalizedGroup Analysis of Turbulen&#xfd;e: I. Basic Theory". Journal of Scientific Computing, 1(1),pp 1-51.
23

AEP Cook Nuclear, Plant Mixing Zone Evaluation                                          April 20, 2006, Appendix A. Current Meter Data from NOAA/GLERL EEGLE Project. Data Measured at Station C4, Moored in 11 Meters of Water Offshore of the D.C. Cook Nuclear Power Plant.
24

AEP-Cook.Nuclear Plant Mixing Zone Evaluation.                                                         April 20,2006 EEG.LE Lake Michigan Current Meter Plots Mooring C4 LatL414.99   Lon:8657    Fle;4-1998b-11M.dot
                  '20 10 E
U>  i;
                  -20 30' N
E U
20 p
0 0
5, 270 i6        a 510                                          320   3,30 Julian Day 1998 25

AEP Cook Nuclear Plant Mixing Zone.Evaluation                                                                                                         April 20,2006
                                             'EEGLE rke Michigon Current Meter Plots Mooring C4 Lot:41.99 "Lo:86 57&#xfd;   FRn=:4-1998b-1 1M.dct
                   ?C C____________________
0 E&#xfd;40 20                    '.
                  -40                                    4L_                                        _ _ _ _ _ _ __ _ _ _ _ _ _ _ _
E-301 E
270 la o
                                                  .34n                                        350                                  360 dulian Day 1998 26 o

AEP Cook Nuclear Plant Mixing Zone Evaluation.                                                                                      April 20, 2006 EEGLE Lake Michigan Current Meter Plots Mooring C4   Ldt!41;9    L&io.57   FiRe:c4-199b-l1M dot 20 w
E    20 N
                   .30
                  .20 E
10 20              V
                  -30 40 30 20
:E 0
                   ._4:r     _._,"
                 -40 .......                  __-___          __....._         ____-.___     ___ ___-_ _._ _._ _"
S a) 20 0;
                                                                                                                 "25 J~ullan Day 19&sect;6/1999 27

APPENDIX 5 D 2688.
4ccatgbuttjotats)                                                          9. :Parity of" Reagensit Dejignation:             0,2680- 9 4 (Rd-PPCO'Q                        i9~                                                                                                   ;& C~              ld Woritng on Corrostion, oec                                                           9.1tnRegeatgade ehemiCult shell he, used in, all- tests
                                                                                                                                                                                            ,           Cold working can beapotiant Ia Caming lcel 6&#xfd;.;116,1                                                             d        Unleas    Otherwise indicoted.It in intendedthatall reagents        astall confoarto thespeclflicatios.of theComdtlte Con               Asalytic.l
                                                                                                                                                                                               *.d'i           however.,pastic defor                 cn bea mnried it             R011ga   ins,of the Amertcan      C*e*ecal                wheresnch
                                                                                                                                                                                                                                                                                                                                           .Society Xletten  pepesioa by following proper mectelgpactining                      specifcaticoes are avuaiinhle.. Other**ades            he ud, pro.
bmy Standard Test Methods for-oHetTasO                                                                                                                                                 eample, drtlling,reamst
                                                                                                                                                                                                       .(for                                    andctting aI orOsIt of~Watrin the Absence f                                                              et rnse                                                             . et M*h. o A                        the importance.of prop.preparatio
                                                                                                                                                                                                                                                                                'vided it is first ascertained    ti hat anregene     is of nsffiecutldy highpotty to perimiits use withoat Iconinlg thenocatneyof (Weight Lo.ssetdV                                                                                                                                                                       I'stegt,recigniaT*d in the other lestmethods. It is
                                                                                                                                                                                                                =c
                                                                                                                                                                                              ='*ss c rporeerr e"in sm=eed rm inth*e pip                                        thedetermination.
9.2 PtrflrYof 'et --. Unles s othelsr          ie Indicated,    refer.
tten wded Ii bisned .dae '60a idtnla&#xfd;-                   tea X51101         e I         t        idatda6tw               -dta     , -VF     IL                                     ' iss  t Methodia) since these'pecimenstitn havetih                    *eces to water Siall beunderatood to men re*gentwmet
                                 .A.io eteleat~
ni-    ~        ~~Y nana e~t~d n Ofena deiedeatd A=
naeeA i "t LW ine ean "1We iipeienotn anpei innti1O.                                                               sanprpeslesan     he ipig Yntemn       bing tested.
co nform   ing to           of Sp ocificai onD ] 193     .
flatttaWeasen qw.                                                                                                                                                      $._ypes or Corrosiona il     GenI
                                                                                                                                                                                                          .           Coroxae is charac-tined by onifotm noick                                      TEST METHOD A--Coupon
: 2. &#xfd;RaeferencedDacuittent                                                                         ee          over the entire Surface.
10-Summ ry. of Tst Method
                                                                                                                                                                                               ,71 Pifning in a farm of localized contusiotedph 1.1. Scope Thse,            methodscover fth determ*m*tton:                      of the 2.1 ASnhfstandarstl.,
A 120 SpceljctidjoO 1,Sellakan for PpeSel lnhat o-t-i                    ~anbr, sne shpeand distribution of piwsbelng pertinent                      '10.1C*aeMy prepared.           weghed metal coupons           are In.
eueo3vt sof wter by evIttt pitting andbyincustermOthe                                             ZlaC;c.Cotcd       (Grjvamlsxd         weldedand SemamLea,             far                                 It may beevaluated      bycoanting then bevn     ,by      etalted  'in contact with flowing cooling water for a measured t                                                                                                                                                    length of tie After removal from thesystem,              these  coupons is afrS of localized                                                                                                                Rthe se. 31101A             enddisorihution, andrby o easuri.g di aa sy   tine
* werighweighiestof weitfl stsfll essa  amelOa.
mI=..
                                            . rocas Pittig ut-0-of the average s~ tn&#xfd;rcotoin.                                                                                                    .-N      "I f Pitain repreaeatative              areas.Both sides    iacataUs.        mu exammined.        cleaned,end reweighe.& Thbsea=Mity and Corroslow        wegh 101     ...                                                                   D. 129 TeermuiOgiOy             Relating toats                                          "'-6~hexesined in'Teit MethodA. In Thaestho
                                                                             . w t is a                          0.193 pecfictoOfoe Rkengetat             Wates'                                                                                                      h rate/ .re t mot       of c trOlon of a metal immersedin'-                                                                                                                                   in            as h10   e cut longitudinally beforeIeternal examine.
raidtng dfife.clae the Waterare determined 61retrtiao in welghr. thedepth    and  distribution of pita,frt,   rha and the to             ttntlI*          a           D 2331 PracticeS         fair Preaain          ad P- lanO7ta uion of: Ue tendencyfotrthe rati                                                                  "Woais.petmmad         Depsidts 5                                                       (or plimiugcan he perfaitaed.                                             welglitand cheracteristism      of tle foreign nomaantteche Coupons.
sots it coat+
funcion of the tendency for water a.d the                                                                                                                                                         Anyatemn A2               may he devited for grading pitting()
7.~te~,Cnrrtnsnin         In A Pdrtinentftctor to consider in       IL Ieterfrevxtee
:    to                                C.,,aAY'r,%"                           .*ow,,,                  3.1+    l~D tOU:.L1100'mo ....                                                                                  sinceactive Corrosion
                                                                                                                                                                                                            -~~pJ'testing.                         sites,may developin             .I1IIDeviation in metal Composition        or sutface preparation Mehd A~    ~~~~~~       iF ltsw 0at 0 Binvttat                  31D-itr~t -Per deffliftida Ofreen ttsedIn dire$,
dodo                            0m                                                        ib: ut C.rie ton               my exist at threadsandjotots and          of the coupons        tny Influenen   the precisio of the result.
A CRY   ad B.,W'y~m    DIb errsaateato rteta-W4te       w                   1103                 'methdj referto Tcrt tin gyllSD                  1129.                                           aepaits, as well to incorrosion apecinmee.                In Teat           1.1.2  Thi preseaco    of differuat metals inclue proxalotiy to 7
ps.1. s; 4, 9)jgifesitadU                                                                                                                                                    A tevca aoslo mayhe mnevidence wherethe an.ne                                                                                                                                         stheCoupton.'(Within       76 amm'(3'ia.)),even ifthey mceinmblaed re.t.ngu.lr pe metal 4. ! Si 'te                                                                                                                  a~~l'i otadto the holder end at coupon'maakings.                   f'rom   the C oup    o n.co sit utesa o-surce  of     i in       re s ults m       .
1.p .fl which aretA         em ploys mounted         t       p            e                               4.1                  tw tealdcesram insepaable for ama
                                                                                                                                                                                                    ~ lalre 4                pecimeansurtur. are reintive to Usecrevice tcd~es'tis   nflonc:on the overall corroolonresutts.            coupons      maylalteenrie the preclisiaaaf the resulis..*
w hiphar     e            mem* unted
                                  ".fowori   aliigPipi
* nO                       -'ld "t-   --- d        corroinhibitand   far water corrsionat      the   thetostablit andcosrtvo                      sst p stoI ocontains                                        is ecsayto redmove              edge of ecaupon mark.            11.4Re.lis awedirectly courpasle-only-f.ir the.wate o water sy.sabe, eISitrie               ttep*                                        "-he er ipe                           sivityoftaitms                  deter*mieda     in"aelaie.
in         r       th                         1tiThs MehodB. resusbject t~ocrevsce              catrmsossrie       temperature       towticih thecoupon is exposed.
1.4..Tes o-'~Mitd.   ~
m B apud      ~~ s -,*tmmicl&#xfd;.wtb             tildig a sa 1              O=
rso bs e, t-ne                     tedec
* l f a mres OfS materialmrm,**.
n slo ca.t
                                                                                                                                                                                -.                                                                                               11-5Crevices, dzposts or'biningical growths my efaect 55hich ae insatlled in a platst PPe             picin as                 t            to                                                                                                                                                                           loac   o teoM       ,-VitY:-'raseshould  thereforeheitrtdwt
                                                                                                                                                                                                                &#xfd;orno             TIMincreased corrosio andyGown a ctl                s.. ,+..l.Cdsl.P*.l~
uraet        *nde..oa me P i~Ce-fowdietoeslon) ipn .ttstentln                          .&provided       ia ....
result, and
                                                                                                           ~
vtol**c~f*
absolute.cop orapting        it with theerrbio naewae.4bnviunencntvervely~
rates   OfMI*,
i:n]
ItsF'    -t.l*ma=
other djrul arouloaapeciinmv.whore-themeal mayhe Iof "I.tnkena    - anro on ae etron an contio pI                                                                                                                                                                                                       Sspecimen Of a high ratio of asurfacetan'to              12. Apparatus Coroston Wansce formed 00 t. Usal                       .. wal*be
                                                                            "    the     toe    to       of           "w   trhed   t    "intd    byte    orrosion                                                      cthiseffct In TeatMeshed 1.th* edges am                   12.1 Coupon.specimeaA.'pre--               couponsaaccrdance jat Occturtingin-Usamtal piping*y-ee being tetd Ste,                                         aaeit                h jateri with theeasts in                            m       e        to fti                               Ieaf*ld*     s*iaL. If anabnormally highdegree            wih .SeM on'14".
gavne steel.andsolderedcapperatid copperInsertsh *ove atrial In otherwa. Sse teat to oeidst.r                                                               fn"ouranp                            hinis   t observe t      intecsScin1 n sheeaseci TessMethod A, rho             12.2 b-Insulaig llbrterSeret            andNat-Use for          c hevatnizd Ston .c                                                                            evaluating       Use  effects   of teatmsethods
                                                                                                                                                       .                on sefC
                                                                                                                                                                . are intended Corrsio Witrtulsod.tl                         of, waler.Althtoagh          these                                         i                                    enon by mneajsutement evluated                         of the speciment     tecuo In th heoi red                           tsaigwan-h             has a 7
iiito.us so0     antifollowing exposure.      Use of 4            ve thfifts           thecoupon
: 1. fatstrded      oe ntptirjortl toaddtesO a~ll91the ain                                 the                    ofwaf carrusrntrt and intos1t o nt                               55 hieains may a'tia reduce edge          mthe fec~i" sa 12.3    *Ph*nolicintoRod-      he, aholeanmodth I5-mam '(6-in.) length  mw of with -st rus. 11It        ithe           delesralingiicoisosivesena
                                                                                                                                                                                                                   -       A                                               ceovas-hesed          13.m,(0.54i.) outside diameter p+eotu.icr..
                   .....f    g-pir y                                           aeeo          se                    5.1 The sp. ece hl                         i,      .                                                :Sr-urn  ivolvd, ischaacrerhed by chitiuonima pipinig    in the.system in which the ousioesdIn                   1ht                                                                                                             Oar ana.Anedsa Caatde-Saetey 4-VtentWa A-s,a bast neet. se 26,13.                                                          Welded        as asammeaS,      pipe ehI he ee In beSts                                                   Iandiheighimetalfrom which protective films red away.        Someoander-ung alsa may he             MWnto. S. A-ad-e Ctaatir Sod.%~as Aeda, sak,deedp' ceaw,,
                      -"                                                                                   however,batt-welded piping specim.ensmy*.
ween an. n atends an aerbi eajedndtrtaP a,.. nt at .                            a       lMm*- .eh   .d,* povids.cu..e is aheo.             to pick rmudh..         ,
tt                             t a,      a d ti                                                                                                                              'AWttdhatntnt aSteeflao tnt N. Ittrine Ave. GihMAta. 10Mt~k n at d outAi.F -,,n w .a                   d tt    -  t t aq ...            'te            . .. . ..                      , O* ,t                               5 )-+                                                    ued deposits       Obhservecdonth specimeas may        nenvud tiee -nt.e te N.a sone    -A Fi Na. 200.5225 thlemettsliad      llstedin PracticesD 2j331The                                                               atndwnad byo. C.
Staiteii         Ietre                                                                                                         8-timadn RMAati.eLt(aPan N2. We50Cr          h.nb.n. rbatd anhay 16tti"..
anentu-liAen 4nnAMt~                                                                             ) alasdWMij      Will he calchim,              ana-kenosuw      retina IThdanantoe              roteaatdn mianr 0.2 a ('
on Mi..                              lb"htaid,
                 !Th.widu-a nt~i           nt~io~t~tjsnn                                 aemtt                  'rm~n enebant,
                                                                                                                                                                                                                                  .dsn       phosphuere          Chin.
sfulfate,                        Sleana~~~~tA i.latOa bai40o                      at           ~ ~~
hiala
                                                                                                                                                                                                                                                                           "-heedy 1.5enM~. "t atit& n&#xfd;hmeedsiadni-.4 runt[%hmiend s        in'iad as
                                                                                                                                                                                                                                                                      -213
                                                                                                       '212, C

                                                                 .&       D 2688 1o0.veflg oflnlti hlorif(we 11.5). Dilutethediorslting (yallow3.Rinse quently. The sacetively first and second ibath- must be renewed fire-I L with wate.                                                                                      in isopropyl alcohol snd bnie*oa a to M  Tiewmerpqlene.                                                      and dty,with a clean cliol.' tore in a desiccator.
3 7)o.l--4Fineiy g                 -nulated.
porous. siliceous rock;                 14.6 Cleaning Coppie Brar- and CaprNickel Cousp r-bonssiica (SI.*, sof porous, and f'ee of Shirp .edges suitble cinulvre.type clann agent                                        w ill~dy (fee   14.53).and stare coupons encti Sarfor fotross COUPon
                                                                               " 14.7'Clnofig,       Stainlaes Steel Coupjor--DsgeSasa tVapor Phooianos   ahtor Papfr.&#xfd;.                                                                                                        with benzene,; dry with a clean cloth. aod passivAte by. imersing in upon"repaorntls                                                     -lidc arciduddcluomatsiaolhiiso (Is" 13.I@&#xfd;a 43 to 49-C (110:
In Ibis procedure, coupons are to becmae, principally                to 120F) for 15lt 30o i rin*e with water, thsn benzene, dry.
set hwevr.
secl       n afewcase,, aswith ease iron or -with a clwo cloth, and store in dide*ictsor saje Itmaybe  ecesar toprepare coupons&#xfd; from                      14.8 Clsanin Alusalnm Coapows-Degeas, with benzene sad dry. Imnoteu*in HNO1,(usp*p .42) fora mosimum Of 3 Use a coupon size of 13by 76 by 1.6         0n (05 by 3.0            misutrern-a temperature. Rinse with water twice ones with 251,a.)forall sheet m-Is eanda 13by 76by 3 mm (0.5
    ]:. by 0.125.In) for cai st as. Other sizes are suitable,                isopropyl alcohol; anodfinally with benzenenDry, with Secleso lowel and store inso desiccator. Ifeopuanis not visibly 2Stbs tota area is about 259 mm(4 lan.' theprI1 IPe                   repeadthe procedure using submerged scrubblng with-achlm,&#xfd;          fiber nari eft. being to keep-b    th itarea large- compared to tha bristle brush in l wter               a      rise.
                                                                                  '14.9 Cea:,12s Z*nc or Galv*l-nied Stert Coupons-ti the Sheet Metal Coupon Prevparvfivir-,bhiinsheie metal surfaccedsinfree ypi d1aired except for Stinles steel; use cold-roleld                   lealm towel, andofstore oxide, degrease with benzene, dry lit'ii ins desiccator If Odd* is-present, polish 9of lust No&#xfd;.4      Spotsfor -fareaus meml. Obtain Stainless steed fini,0&deg;                             . .         .            with fiber   Nor  0 brush,emory   paper, scith InIsoprolyl alcohol using a stiff:
no  dose   In bce.tnc     Dry 14.10 CleaninI L.ad Coponpr--(Spec.mens  and starie n detsiemen I Sheer 54-gag SlieetMetal matetla to the dimensions                                                                               sdhal bh mm    (0.5 by 3.0 in.).                                       handlerl-gently      with r-;S t, Drill rpAtiMcaSmun (0.019-,) hole with its camte                    ,itomizedwmter,           plestie-tipped then nmers inglacial   tweezers). Ftirst, slotse is deie acid far 30 a.
om (% in.) from one end of tlb coupon.                             Rinse off:the acid with flowing deoni*.ed water for 30-a; MDbLUTr  all Sharpedges mi the coupon spicimen, using              lmm,*eein scelone for 15 s; d&yby laying onrdy tomwel:              sito rovery-blth sod dcbotr lbs bole with en oversize drll:. Is asdeiL-ide fr-l h before wetghting so 0.1 mg.,
                   .Saplstfng numbers or lseonbsCoupon.
6w the emmitg'hog    ba .                                           1S,. Pmecedore Caq-lMetal Coupon Preparatila-Obtaln                                      15.1 Weigh the cean,. dty specimens on an alytiesl rough cast-.
be desired m e*t*             1Arsbhout  19,by 114 by 6 mm 'baance to the nied 0.1 mg:
Vaby1/4Abi.) from a commMrcdia         fnmadry or elsewhere.                1..*2After weighi*g, als the spinc iens-tia desiccator
         '-Surfte glod to the dimensions of:13by 102 by 3 Suntilready,for use. If storingin D desiccabr is inconvenient or
        *byA.0 by.o.1251n.) ad surface tughness fbout                       Impractical. use as alternative: method for 'roviding                     A "Drigla 7-rmm(Ys-oi.) bole with lb.ceater abost 8                ectrmslna-free       tatmosphere.
15.3 Store fen=uns      metal coupons In separate envelopes reil)flrm oas end of th coupon.-
          .Debutrali Sharpedges on tbs coupon specimen suing &#xfd;noafekrous   mode from metal*  vapor c0aeihmabthor-Impregnated           paper Seers
                                                                                                              -in sealed plastic envelopes          o enict bell,4sod dlebiir the bole with so oversize drilL wrapped inspladic film.
        &#xfd;S.Iion ldctifring nuebers or, letterf on dhe small                    .15.4 Attachs the coupon, to the phenolic rod, using an ttenhfcbedge and therusnating hole.                   linslating washer to preclude any contact of coupon with les
                 ~'sppr~imam   weght ofmeal&#xfd; coupaont8. is a               screw sad nut assembly (ne. Specificaton A 1204.For lidded peoection, attech the spW men to the holde using a screw en nut of the same metal competition as the coupon. ....
tams                         15.5 Installtlh* holder and coupon massblylin a suitable S.7                    lin or In a bypass piping arrangement as shown in ig. 1.
15A6Adjust the rate of-flow of'at*tr in thl tstrplping to a 1s-sc                   rate thatgivesa flow velci.ty that corresponds to lb nsrmal
                            'e nserr                                       flow inthose pamrtof thesytem under pama consideration.
l C rr onDry. I        ese-np r s'hlton
                                                   - Remtocontainin       Nrmtally, the flow vi" tywill be in the range from 0&6to 1i v ae ll reid Ibsl the enpo bPthree raptd'scm.m-              m      (2 to 6 fey,. Check end readjost lhb flow as ec ay to by ,
maintain the deaine ratem 153.7Remove spechnens from the system at ihosvnantori mm'se ah;telsrnawtrbt                               vals Since the"corrosionwill be high initially sod then fall Is.
a lowir,ariny colstan rate, two time series should he Chmen.:
15.7.1 Use-shore time Intervals for the firt time'i rlesin orderto establihbtherate atabih passivity occum.Riesmoval 215 9,)
_j

D 2688 A&D 2088 prepare. Recently, Severalpepema(I          (12,3,4, 5) -havebeen Aerato tenm                                    .3 rwlhtoAZnei~ the:bakereto factor- to he'                                                                                                presented which make the technique MOrePrecUiCal,, a ccord-t thecouponWeight lessen                                                                                                                 ingly. the test method           bh ee on     mrewinen   to' Includethese Uecoptrnled 4.-tppAYinalhi                       n i :G                                                                                                                  ZV00
                                                                                            .14 Rewelibh each coop0tonthef nearest 0. 1 tug.                                                                                             Iinprovements. ,Essentially,Ithe Ichuogelsfrom e tso ea Oninth COUPON. toeer           we as                                        11t34                                pin i g lud n           t sleeve fee ho using fth 2-Pplasic                              in ects, 100 a 5.7.2 Use. gtn        long toval for lb *acnd tis rrest                            .15 If itn se .)t appaenett                                                                                                                  complet* plastic (PVC)" body.- 71bisimptlfl*e the onstraclin:
ae e                   dploftpl           rersnaieSt with the dildepth.
odrto establish the 0mm it lyeat"MCnteeiom                                                                            p0 depaths.11     ominher..      6. Caleulatethe piining          ubsing F&#xfd; 3:                            and r-dlue         a      ing ea=L The bic Unit is now generally ftre         Recrdthrsst~~lvolmeSS rovadof the en tpnsatrImnhand the remnltng8                                              hae.and flltiuo                  sh al lso be dietetitl
                                                                                                                           'ftepits                        t                                                                             . ,-in. outside dismal 2 -mm) amebly, rather thea I-is.
couponsCSt 6oS-moetu interals is recoomed-,                                      gn R                                                                                                                                                                                                           for Pseparing Air-                                                                                                                                               A.-i; ou),de (33.4-sno).                   rvd.tedma
                                                                                                                                                                                                                                                         'blb I provid,,         the d cleaned.
15.8 protect thespcientet If it. anotabe examnined.                                                                     deteote,  weighed  em.                                                                          13 I   sad reweigbed imomediaetly after ocovlem s hasnet.                               oi             5.6Rends enapn~tce Of the                                                                                                                                                               O tse diae*te*
prtece. "moderate leenliord.- -"snederate Pitl~
ns"                                                              opseetneas                                                                  .O-.
(26.7-nen). and diameter 1eN Outside      diamte -*.-.
(21.3-mm).      (33.4-mra)4s&ebKiS$.
Dry between,Paper Ioes                    hefso sreta  bar iOpnhibtor paoeevelopel tmode fromt vapor Pheeibbtf                                                     aevete~~~~~~~~~~~~~~  PitC. ycntoilteruoswt h              t~         onovert from mils Peryear to millimetre per ymer.
19- SnMMILrY oWhTat Melthod lpaspeoted -paper or wra                arfully io pliastc~lm o                            tsseinul.4.asreas                                         ldyyOll234: ofve         Result                                                  i9.lRemoveble Pipe in*rts maeinstalled. In ploasilcpipe Unfentrou     metal c~oupons,MrapcarefolY in potcflm h                                                                                                                                                                          caonwed by piping tinjons nedza made part of.thipopU aPeriod ten between removlofse                        ew Ingig .n            CalcountiltI iteraim                                                                                                                                                      It, Should b~erecognined that the following -deviations.
shooldbe kept to a traitaUrn end 10 aebal teer s 16.1,Cotoslon     rns mu selly calculated as so ave                       t f coupons sed fthcu*csponding'a               -titrllof con-     system unedr Mt PmpT dimensiom we provided tbroagho S      as that Strentmline      flow (eo-flow distotion). is preryided,      in teag week.                                                                            seontin 10  4 miePer year or tmilisret50 per year annu               dsony lead to thefollowi*g e               .eoInteMtr otopi Iaoprent       and th                                                                                     essembties in Standard        steel snd galvanized sod copper tubing.
15.9 Estion. fte specimenOO       sod eor either by photOgtsPh                                 " ~'-eat                                                 I Deviations in composition       or surface   preparetion,                                                                                       of Inrereni Is now being shown 10        initutocr tetg'itarolon orbyddtesPtOn the AppWhesroc~f 'be SPe~tcimejPybtt                                 rod.ot is general(-).-                                                 2 Deviations in Velocity end direetlnsi of flow;.and 1               other m  , e ws;such as l aed.      for exnm ple;ho&#xfd; avger,       sipTrc aae  .
parllcds attention to fteisamount         sandasern ofonay dethreot                  16.2 CtslcuaolOti ftof isC rsr"ona Row                                 3 Deviations in &#xfd;crevices, deposits, or biologicalI                    testingof th Corrosion        relntust  eof  lead    is Confined        'll Wto deoit ttr       s~     'olf fth deposit tMAY      be-perfosit.d hot thisetep is Optional.                                                            1612.1To Calcelate th caFF as9 te(091010m the    following     otter       nifOr.eC;CCoOPOO.       nse Eq 1:'                                                                                                           Method
                                                                                                                                                                                                                                       *IS         A.end difficult   becasmasy the aotuniform be r *h   peeparati Soldered wdepouIble. ,of lendPipe         pipe Copperhacnmti 15.1     Fo ferouscoooos.oseoneof estivoptedoeforde                coupons gth Use0            ior to reweighing.         y                                                                                                                                              tssen ore Presentl ente                     xoe nhcSraeO h lea tls inotonSsota 15.1.1                    w als Possible Weith#plostic                                                                                         ,,cs iOn isn fu**tiron, eacof endividoalsyMeto                     Prepengomin    e attc             ncaucrvccoxi acdZ knf.Rtove oily sod pansnydeposlitsby soltuIeg Is _lb                           wei-,Fght loss.tg.                                                        Sage*e 6t this time.saltemet
                                                                                                                                                                                    *al       regurdingthis prope-ry    tld -ot        'there is tsondie             of the corroion debsr e mea0 knie-hleORettov reoea-t loose otrmsion products by                                                                                                                                                                             Loin      10weighl of fth*eioew is onleraeura of the averag If = de.sitYof the of   metal. /vm sd                                     !ii! Is a comhro.tivIe type test, for which prcrision Inuohiog with a brislin brush. lmjtttft6 th`,Pccioi                                   - ,=epoks'ede          COUPO.hL.a,                                                                                                        conUson per soL arUa.Exenation of the surface is maddeto
        &#xfd;inhibited    Actd.using either of thi- followinogtwo tecrhatqdn                                                                                             a.oniolonte.
Maeerdesee,  Thetm wataer    ors many quality,         veriebles..'umc and fth  Presene of othrer  as    evdua= pitting. This test m d eay -hboued m de.i*mi the 1AI        I-Itlnmearsethe  4-111,11-   nuthibIted*         l(+1                    16.22 hcabcolate~       crot rateMensltuneTe                        a mayj M .lao      the raie of c erotosio of the co upons. degree of corroson o'cucing 10nColdor hobdlstrilbutlon water for 309 an.trmsomcmpeltstl                                                         earsfor eachtcoopaonuse Eq 2:                                            the composition of the taut stetal sod thddifferenj                sYe.,m or coolien water Syranm.to evaluate diffe*ent metasds-specitmens    ts   lohIhited      KfS0                                                                                  secrslta hi~chcurt oicrr. nitch-03general ememoion. "f chemical trea*itent, e*ado determine lbspropmchoce of A 15.10A1.2lomvmrgethe                                                                       C tietletl{t~iuenOO5P ugs.tol)  - 3aSS taAWA 0 11~ (l0"1~ wih ndiuect current source (1+3)                                                                                                                                      Wtnslrohiologieel type. may affectthe remsusoppre-                          coerolna~reisrntmtalfor. the syutem.
imposed 00the coupes    4      asns anodo.sdte          ssebd.h 2.5to IQ A voltage sh Ildbe to s V sod the Cuterotlenslby3                                                    of-the.metal, g--m,,                                                                                                      20. Apparatust p.erIpecimen5.'~    K     bO5 e.mIiorn tshehth for to 5 11111 d =-deoity area.fcou4om.sod the                                                                                                                                                                                                    20.1 Te-ter As-ebl.-: Th assembly cnmits of two in-o, =-- expose                                                               'MODB-.-P~e 4,5). Insert InPlead. Pip.,(,.2,a 15.10.2 R11t* with vituer, after emmoviniiipecsenCs, front                                                                                                                                                             lone. Constructed from aepresentative lot of -it*n.ooteide, lsPO Ir04 A,                medyL.
                                                                                                   -re                                                                            INTRODUCTION the Wlobhiedseld both.Rub apoiotti wih then with trlppli.. Rinse, with wo'er.Yto5O with jBoptfair                                    spt"tC graVstlnso eitnesil ar TI3Mrh elcltl.Drybewen pertowels. fllowed by Wueosrm                                                                                                     ethatiof corrosion testiag In municipal distaibstian
                                                                                                                                                                                              .Sadffectively for, many.'years; its" has b*.en&#xfd;                                                                     h V-         pv cs s,pL           lts latt.             . 8,4 4 5tn 21*Jtn sonigth olowing                                                                                                                                                            .lPVwrdh b Oti th assembly has born cumbirsome and costly to e   ~           1.1tor sppcr to copper atlloycospon$.&#xfd;S h                                                                                                                                                                              oaW  CMvftokensiTi N. tows' 4 Cr5en~ea otek 25471.B.L precedurte for Cleaning prior to reweihlf.  -        CRemovteciyOrupeos as weillas possible wvitha plastc koio eov iyorges             h cup deposits by sooeltlg UIfticatomnthy~ toofle                                                                                                                                           TABLE ISp-cutlesstlov 1IcG TowylpeCeroaton Spachtnec.
in inhibited HI~ (1 + 1tR8)     er 30s flss copot     C14 wi'thwote, Orats                             .          ar.                        "Ire hse               On.tsdd Fn!W                          blt Drycoupons flitweenppcIW1a         ~           ainsds~~O                o nersees Me   p t'.       st00                                                  to@ee sAo                  e                        a ss aumloO5 15.12Pee                          lw Coupons.Utoeth duroloolots                                                                                                                                                   o                       4.05                     nasa                  0025                        50.7 Wh.V  .k,5. a.
fOllowitagprocedurS for. cleaning prior in rawlighitig.Cleen
  ~~           the~~csepoOtosttpossible0            WithI'%lastle knife. Remove etnod~osspp              esseetad                                                U 10                 105 LOWa                   to m2 oly or greasy, deposits hy sOakingiu uin al                                             tridtloosyleC.toC ai solution mrethe coupons     in  cItUtelr aCid~phesphorile (see 113.2)1  at romtttotttrfo 0m.Rmv o rinse.
6isoly is ithlas                                                                                            aeneas 4s.pp.            gukWrdnS                    4Mn                       1037A                   1r12 ,                  t'3.0 with witle, rinse with isopropl slrho so.                                                                                                                            Type L tabi             eavs                           Coo5                   lS                    t.5512.09 h.enorepcDry.hettten aerlwet     Pi         ous inn de-cceto for 15.13 Subjecsa weghedbleank oponO h5ofmu rrsiniprcedreesd frthe test spot tins trshcdeol~l                                                                                                                                                                                            217 216

                                                                                                                                                                                                                                @      D.2688 0 2688                                                                                                     .5oaa                                    'TABL    E2 Factor. f Wr    Conosttl uW Ih Noft 0.ls-   .reo.                                                                      Lass
                                                                                                                                                                                                                                                                                                   .M a*aromeal s into 4
                                                                                     -        "(Spe TlrAiHh/orrAeid (3+4). I-hIVbed                        ZO of el                                                                                                               I, Corrosoon       M      "'
dinaneter(26.7-m5t)                   steel                             etndr ppei ad. ril (Smi-pip                    y,+ Or1Ar"                         .. O -9....
                                                                                                                                                     .       . -...;l F
ctedby mreachul ceue4Zlft                                                       _2.)
aospip.             M. 1 sl e             em            -W, A re        A deitrfalbY In 110.21cibly) or copper,  ieatia pipingS.       ljlblvcn am                              yajAC d (0)R ,1 2 1S&#xfd;SlyiawlMdI 2j 1.1w*m<         e o S .atpaa                   . acento Poem tV A       00                     .. l ..      .              .                      "I5M   05M.11%"-
head in place by 3/4- , 0 i e da ee.*00                                                                                                                           lds ItI..eo -jAc               tad 4           15daea        1W          574 .
    "showun1 f.pi.3       and F 4ii s1                  )U7-rl4S eroittl)PV                 se             eluF_(HISOan                                                                                                                                   1. T*' L  ISll(a                  t20          to.          4.91i
                   .italledina t 1 sa3/4m (27JnSetoeO~
scettt~                                                                           a.       ad s of aticyclit abietylatton(Se2.) n i socket Wem,                    ) 3/4-in.4  (26.1-naulan 1(1 16- dIa     .PV uion  (S4             -21. C.d::O'6 5           Mj inm:     90 -... wae....andi 4c,10 _               .+
sbZ               Svhiatidnn..Dsn             g ofsode                               .014!asaca leca tlY 11C      hv e(21asoi at       consttructed     according tO                1         2.P      0                                                                                                                                   1.          40 ASIeddl. sta pipi.               2120         0153         10.40 7,Si   p etfpa Scssu.1
                                                                                                                                                                                                                                             %.et       L          t s t
* 4 sno oPMM~dPoet      pa p pa.o1'1517        05 II        now 74.0 -
teste.. Rose                                           d e U1.S. dArmy              d 20=      f die c ors nmtin                                         t'                                                                                                                                 ers poxyte               517pox
      )n1Tablpe   2 oop"I              in        bahlbTest h-tethods         AIssli d            ilt   tBILDir~.~  th"                              015.O t5                                                                               IApO05d15a.S               p r adp aepox hold
                       *PTulk
* Is                                                                                                                                                      Ss-
: 01.       Ot0        dealpip eor                           Ma5a         &40ta t mater f.bettetr s          the   *"           psasivs  tht8S    clt     - D.                          llyPOd
: 21. 2WchlroE' 1 andhot
                        *cold
                     'etsnma
* water syste]M.                                       iaystacks tiaald!0 5
                                                                                         - 22. PrePnoIoofle'1n-tt5l h L*
524.w                   rao*di tpper s    ~,a      3         55 20.2'Dial DepthGoig- Se 12.5 . 150-W. 64(
        .20 3 U71IrOsn5       le,                                                                     I MrtnP                  .2arlisumba~lo IfI gIgttt~l                   ~isorielaee tn-tt&#xfd;t to held 2-1.beaker."ilanal lack                          -Ltbcdin.                                                                 deg`eneLth insertlsby itafT                                                                                                  05.OysiarOtia      Oc 00,.                      147          07            58 22.3 Theextecior and eadsof the macalaare coated with a
                            ,?,L " Pub a22.                                                                   thae, nifeol ainsert        show the&#xfd;prescflce       of rust etrof                                                                                     e epoxypOirL Blend the eLoxy bannandepoiy py W.
2121 lyrt    A        btclmtlo~                  ed(actaldthl                          m tedaya ise notremoved,cleaincopper                  sadiatel latest                                                                      W..1:2.
Place catalyst     Cmm~
rubber
* stopperstoby-*
according          Into the the   vhos ends npralm       of   pin sadthe     3" to hocarts o*anncfnur.ninstructionw +nprotect
                                                                                                                                                                                                                                                                                                                      *- be eseo~deadd~tif     cess                      ~
a end-otoesndd5l                               with (1+3) b~hydcblonecid                                  inwntilfref Inibd             &#xfd;,
produce galvanizeddspI       - by~the - 1,:                          Isticmt wtw~a Pvc   wen      o          -inteior aS        roil dry-flute surfaces       darinIthieloaras; pase coaftngAflow               IoAli dry feir res t hei nserts to ditht~SOni n                                                 so peve salto nsto         -                    surfeca            Icdb f r sUlaniomi                  oocaset~ ttahtttr,5                                      . P ac p ed.r    sonti ot lar   ,eCe in xpose           e achen
                                                                                                                                        'loP       d       H5tie Of art ina  ti lfrwilmstfr CortaaldtIIihb                       foro y&#xfd;- a, fe                            epm epseInccetae                            CfoI                                                                         24&#xfd;22.4'Stem h.and remove   mciraria theinstopperm.
a desiccator auntilInstalled in the tester t         ahoev Coemesom ptst                                                                                   m ay      eiea.mush longer reute                                                                             assembly MFg.3 and Fl.S.41).
2f.3 Sab1s1tci droehl(tIt 6cid.,Arseenil                                                                                    a v stpp rS.ivusinscSwihwtt
                                                                                                                                               $               ~
3 Procedu.re in,,
plusand we a-01.tarM Ulsle W
                                      ~~       L50e..
                                               .. ,!st&#xfd;o 1
                                                         ~tt,%)
0,PA      teloa mete P. 1001t55Wes
                                                                                 . ,'.a 16
                                                                                              . rie eo       a . 350. n.t eeep ow by -LOflW
                                                                                                                                       . Iam . ade
                                                                                                                                                                                                                                         .the system 1
23.1 Insilsthe les assembly.
roife obsenedL i      a siiln.ainlzed pipe  lina in 23..1 A miiataFr straight ru of 91mM (3P                          fr) of piping Shellproceedtha test as emnbly                  topravent undue Dow distor-23.l2 Cossstructa don.                           by-pose valveandpiping sarreangemen                in oider to allow     insert removeowbile the syatemis opeoetieg.
ac inld
                                                             "(*      dshte      Ilrcyi!      0Ooatngso' c:                          l;.                                          1Weei+                  IIltAmt5e*Q 1. 05*0-P~jo                                                                                           flowrate Ps1 e ordir 23.In  etoobtain InstallCUmRxMm a Watermeter Informactions fhtlowiag~    te testthec Cocernting     assembly    in:
and totaRlow.
23.2 The micomomriacommanded                       les' petted Is preferabily 120.days.
23.2.1 Removethe. downstreamo                     Insert at this time tW 22.12.2If feaaihla.exposet -he upstream                 inaerasprefemblly     for 3/4'(1.91 CIn pV'i~a rjms, pipenipl4 PC                                                                                                                                 a teatpperiod of`12 moaGuh&
24..Calloteral Oint 24.1 &#xfd;Recordpertinent' Information on the physteal and s2.Bt~~    ~4I       l rolftedati~g!0giostai,   , PVC,SeetoSt                                    .
chemical       chomwedseisse        of the enviroement in which 6thelet is Rsdsmd O.Oi5'lOj~q8e                                                                                                madeion the seatreport sheet(FigS6).
214.2Inclade 0130the locatian of thetester,loeen materal.
Record,   wgla and in*s&#xfd;er mr ta              et       hisert nomber. installation' data,removalidate, andother 'do.
tep.......                                               ectipliv Information in~thetearrpst she(Pg6.
w Caress~   Spocltsm              j%4C      oil ReduacdWcl0en cr~eal a istadttgdFls SteelaPtpe alwhO s_t .tersan   rapnsaivas mat sat     It they re ass     2S. Inspection
                                                               'o       hie frocm i net v.                                       25.1.Diver thUeflaw of waler from thetesterandtemove thie hodfl38einl.Le40ol                                                                                         P . ta -                                             Insert ficamthelost assembly.
                                                                                         .03Ll..      ciil                                                                                                                         219 1-f

                                                &D26888                                                    ns~tltctionno      puat Lo000cation                _________________
Type      tattt.ori.
DateInstaled
                                                                                                           ,DateReported,
:onoacnt.
VittlogeRvsaGlnn,             Osos______                        50soz'd IhaPol III pxa~lloum   -t 42taft..imotallti.lc         10 -1*l and coccosouo ptod.
                                                                                                                .*3)ater la          to1tS., t IC..         cO    WtaI   005. os 9        V/say (A[Oaln .. sees do.Ing Lnist.110115 (1-2)                           -             -
()laos.o SCRIG,sd c~orosoni Press(2-31                               -
IC, 'fightSeale and Coeooalvo         pond.      (3-4)-
                                                                                                                ,o)- t0tal scale and corrosion or-da 12-4)--
I01 A~c15fIvaiht 1ane sof tweet (1 WV q/o 4! tutj appotl - 120:v/v Ag. a Ilport Ithost 0n Corrosion Spogltosn (test Met~hod        0)
ISgof Inserts                                                   26.4 After. wihng,(th inserts lengthwise- In bend saw,
:r oemove from tho coorodlog osovironmoot. -dry             lospect the interiorsrorfe          tor Oliningrecording the atnober.
WC oveo for 24 h (except fiewcopper insets whldic           depth, shape. enoddletrilhllon of pits (we 7.2 one 125) in thr A in a dsiccntor for 24 h), cl*se ends of mire wit,          pkt evolootico cohbm shown InniPg.6.Aso inspect copper pets, immer=ieInset Jot on epoxy point stripper to,          Inserts'using 4Lrdicrso1po to determoine if. tritoiens reslling nt on the exterior. mndremove all Its point film         from crosloo3-corosisoony hov,     IVCI -nocured aintoAfter rosovln4 the ostnppcii.dry ogeoi In u             27. COlculation ifinof h, cool in a desiccotor for I I (except for             -27.1 Calculat (ho scale"nd corrosinn products In grins s-which shall be dried In. desieisto for 24 hi),        cod goins=per day, onindicoeld in Fig. 6.
o thonearest 0.Ofl R~tecord        theweight toolne (2)         27.2       rxproo thi mien  .of corrosion cither awcight tonepa I sheet (PRg.6).                                             untsmen per unittime or the equioaolcvrate of pen* utlott.f7e mauln: While removing point, avoid 6ootea with               aeccptcd units ate grim per eupo=roete per doy*fglr2/dey) otionby wcorlng toshbergloves co~dwogtiog In o               cod mtllunrie peactratlon *er '"F(mMpy)MUsper yr po thein          w~e~rwithoptl o to remove loose          (4py): Colcuotn in g.odor gn Idy meyl omdsusing Eq 4:
I woshwith a broth end scourin poder.D*ry the elgbiio previously noted oiler otrippiog. Record the                 clride, da .It?      ~  to,~b steeMW sd elvantrod
                                                                                                                                                                                                                .op-lsen.
ie(r3)'of Fig. 6.::                                                            -61. 'Maty tO steel inse*.rinn freshly. prepared solulon of                              ..t20   wiZ~pprcpnd                                 1(4)
Fdro*hl'ic :aid (s=21.4) for seve] minutes or where*
ttnolmcproducts ore tosoved. Copper Inserts ore               W. - actualwmight loss of inse1.g, and i     c      redhydrochlnor d                  acidforl I n2 mi to r' - mostollorlontme, days.
not no (hocopperrei herb to Clemn 0othtd                                           27.3 lbe relationsibp beomteecorrosion re in good.mnp,.
codt.py, (27.2) in o follows (ne Ibb 2):;Isultply go4 by galvanized Woos libmrse,                 In e n ihibed sufmin Ili 651density to obtain onespy.sollllmee            year Aond ottpy/
(I0 %)l(e 21A.6)    for 5 min  tolIooen thdeposits.      0.0254 toIbtmulo tIoo.The densites wen;lso)am st*el---.i,
                                                                                              .Pous't, by'brushing cod placs:ing.n o Utdraonicl zic (gelvoo'eo-"-7. l ; copper'.-,8,.96;. leed-,1,,34                           "
ttreesosay. In this ns-oe pinceinserts in a 2-1, e.0bclcr ceatioing (ho Inhibileduold end plccW 28. Itelrpretation of Rumnlt.
                                                                                              .45aoOIc equoipenos contaening w            -te    '            28.1 This toot closely sboostetes ortuo piping service coo-.-
14-:1111~  inseets with water and ecolono, dty inoj ditions end hot been observed to yield on accurote mtenum of OfofeI bh(except for copper inseris. which shell he, corroion oceurlog In a pipin system.
for 24 h). cool I desi"catot for i', I.ad en1"tor                                                     s Cotsin rtots of le(o then (0.13 mmpy ore considered 28.2 25.2 Itecord the corrslwt Porte"t       coL sosftOor &f        aneci~tcoltemn of   eIogte:e O4                 g.,Record the weight DP*0o N4 of 1leet0.001                                        low. ad or. ageneral idiio             of eatinfactory Iservice lifIe of sl odppeytl corso u crrrsdn hul roughtcuetOdSM1orpittnc.                                                                                           the ,ietils'teted cod exposed in the piping system.
obowing evidence or ,,,ilon;,SrOOVing, n eoird the oppooei o)4fthlepoinedsurfd Obov                                                   questioneodhecroeo;ee eulSm (ho b     Isttd ItelOt p I g. etc.. If rho#pointbu fledt      qesrte
                                                                                                                                                     ,221

7
:l
                                                                                      ]D 21688therefore, a general statement regarding this property is not 28.3 The, degree of -pitting rnay be graded (7) and its importafice evaluated,.                                                                 practical at this time.,
30.2 See 18.2.
29., Report 29A1 Fig. 6 shall include the observations, 0,eight determn                         31. Keywords nations, and pitting evaluation made in Sections 24 and 25, annd                           31.1 -cooling water corrosion test; coupon corrosion tes*
the calculations of scale, corrosion products; and corrosion ra te in. Section 27.                                                                         distribution water corrosion test method 30" Precision and Bias.
        .30.1 Precision, is a function of each individual systen RET RhE      NCES I4  (t).1"illinois State Water.!Survey., Proceedings of te Amiericahn Power Cotifercnce, ,Vol.XXV; 1963, pp. 696-697:
(6)Metals.Hqibook,'VoL 3,Machining, Armerican.Society For MetI Metals Park, OH 44073; 1967. p. 75.
(I)tReiber.,Ferguson, Benjamifi, Journal of the Aknerican Water Worx, ,                 (7)'Darin, M., '!Corrosion. Criteria--Their' Visual Evaluation,!' Aiflj November.968, pp. 41-06.                                                               .Bulletin,No. 1384 JimuSTy 1946, p..37.
    &#xfd;(3)Prak-asli. Schoize,.Neff,.MalonqYI Heath" and Saith," Develop'ment                  (8 Co'ng          e Moul C             "pe 6-Water Chensistry sand Trriaesa   &7.
the Pipe Loop 'System for Determining Effectiveness of, Corrosio Control Cheriicals in Poinble Water Systems," USA-CERL. Te dmic,                        Con*in lower, butitute, 1981.
Report N88/12, August 1988.                                                 r-     (9)"NationalAssociationi   of Corros.ion, H_,
AwiaanJ'.T.M , VanDroffelear            "Corrosion ngiheer,       and ItesConb4-
                                                                                                                                                &#xfd;HoustonrMIX   1902'*
(4) ,.PrakashbScholze, Jr.,. Maloney, andNeff, Proceedngs*.ofthe Wsati Qualo Conference, Baltiujore, MvI, November 1987, pp. 43-156.                      (10) Loandrum RJ... "Designing for Corosion Conttrol,"? National A (S) Singley andLee, .Journalof the AImerican Works, Augiist 1984, pi                          ciatiOn of Corrosioa Enginer. Housto' TX 1989; 76-83.
TheArnerfeen Sody for Testing and Materials takes nopo,sltian respectOg the.vat .at anypaten dghts assertedin connection               '
with anyllem mentoeted In: this standard. Use of thLs standard are expressly advs that.daetetratnaffonof the valiaity atany such patentIghte,and the driskIof hiringsmentof such dghts, em entirely their own r*spons.bl.. y.
This standard,I sqbj6ct to rovision at anytme I by the respormsldlethnlcal committee andmust be reviewed every five yearn and revised, eftharrnappnvedaOr wjdrwnI Your Snlaot                                         fcmments .em Invitad att erforrevshton of thii sandalrtoreraddilonhalstandarda, end soud be addrassed to ATM Headqihrntem. Yourcomnrents will ti~ae'carituconstdaati&#xfd;o ata meeting of the mspohstbte techhhIca ConMIlttea which you may tlter4 It you feel thaty LTCm cmments
                                                                                             ,      have notreocaivedea!,i heirnin you should make your
                      .daWs known to the ASTM CommIttea onw:Stndaens. atfthe address shown below Thisstand cr4yd  CpyghtedbjtASTfT,.1 00 BnarrHarborDiyme, POBox C700. West Conshohocken. PA 19428-2959,- UnitedStltes.
Infividuailrpdnia (sinrge or multtple, 0plbs) of-ttIs slandaectmay be o0alned 6y ontacting ASTM at the abo*-addrass or at 0I0783-93l50 (phone), 610.8 -9555.(OWx, or sence~astaiaomS         (e-mal); or tiough the ASTM wets8te ( M-nt ,org).
22Z

APPENDIX 6                            CA' O6V&?oa~
Savis'ta I
      -z4 Membran-eAutopsy Report
                     -.-Completed for:
AEP
               ,At Cook Nuclear Plant Avist Treclmnopigs. 1nc.
                                    )"'S, I of))I
                                                  'p 133

0 318 8002 DNRMODUCTON PROdi1DU~jES AND RESULTS                                                 .............................
Wet Test,                      ..................................................
                                                             .........................M.........
External lupecdic                             ............. wo.........................
Thesima WzOP               .................                              ...............................
Tdescopingof Element ca49M  .......................                                                       ............
Bzmc Secal                                                                   .............................
AnfltlSapingDevice (ATE))   ...........        !.!!! .......................................
Penunaft Tube                                                .............             ?......          ..............
Membrane                              . .. . . . . . . . . . . . ! . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Po~wma Testo                                                 ..   . . . . ..   . . . . ..  . . ... ....     . ..  . . . . ...
Feed S4macei(VeIVr)                . .... .. .... .... ... .... .... ...                                ... ... ..
GlenIiest Meubrauc Fmulut Dmf                               .... . . . . . . . . t ... . . . . . . . . . . . .. . . . . . . . . . . . .
Bubble Test                 . . . . . . . . . . . . . . . . . . . . . . ... . . . . . . . . . . . . .. . . . . . . . .
EDXISEMAn~yosi                          . .... .... . . . . . . . . . . . . . . . . .. . .                     . ... .. . .
FMI Anssi                   . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .                       . . . .. . .
MAfcrbiologca emniaatkm     .... ... ... ... .... ... ..
Compabibf test              . ... ... ...                              ...
SUMMAY'MN CONCLUSIONS       . ... .... .. .                                                                    ... . . . . .
RECOMCMENATIONS                                          . . . . . . . . . . . . . . ...                . ...   . . . . . . . . . . ..
APPENDIX~c A-Diagprn of an RO mlcmet'                                                                                                                  8 APPENDIXB -
Digit lfotoiupb JK Avhsfa TerA"nplogies.
Inc.
Page 2-fiI

031 880 0 2 IITRODUCTION AUP spat a Fihute BW3-36M5 eleomentfm their Ca* Nudew plan sit for mauops The Serial     m= of that elem=et was A8141661. it was mpoftlft= ftir RO.syst was exposed to. a, biocide (Spectus CTI300) diut cootaned a- qjzatenmuy, amine 1The systcm &evekpied signs of foaingi shortly afte ft.e inroutoxfn of fthibiocide hwot     m Tfelwpima      Goal of ti mapy, 'was to dwnnine whether Spects CF300 f                   dh The: ehment hopectim, rsults are mmim belw. P~leas.weeir to tie: dezrmm drawing in APundix A* an 6qmuepautio offithwomu used thrughod this report.
1PROCEDURES...RES                      .
Theeknen'sfiergas wrppngwas badly ra         d and defomed. As A resuk~, it ixudd notbe wetmtested.fl o~e ELEMENT            IGHT Because elwxst weigh is ai    A       ndidatie of the degree of iuling, ekmewelts ae weWghds prio to auk4,sy. This elemet weighed 46 pwrxle hue nomina weigh of ne delonrt I
EaXTRAL. VNECION Fibrgasswrp:                                  *
    .TelesaPingof mdeuulaw Bodtnds~ of ftheclomnt were examine it sOpign of: nouternx                  and bd' sla eurusia Th-is typeof dnamag is:tne Iln~rg n is causd by the &wvlopaxun of.hg                    resr (ysiza* great i hn 12 psi)j acros the elemnt. Madeate
         -ooiawas obsenved Boineseakr mebieseal prvent     bypassing of feed qwat  aard the delnimu    Mw brin     seal was in,
   .good     udaitim with no czdAe o tamls observed.
Anadoeecapin device (A4Th):
be&#xfd; Al's&#xfd; aie desiged, to pevWn telscoping of clemnt&#xfd; leaves at nra                ifr~a premsuse No crack we=e detected.
1ie PaTge 3.nI; K 1<

                                                                        - 03 18 8 0 02 No scfatlcs or gougms wme viuibl on ft, aids of ffie. PaMate hdme d&a woim allow by-p*sof fedwazr.
InWENAL MUM1NAUON Mod= %D       hevy fum,, . was sem. "rU foult was gVy in cobr and possesw& a musIy odor, A mmfm saqple was dyed with.rystal violet dye to hi~                       danalpd, Fafjiwwu Test line Fq*nwaI but18'used to damiwnm wb,-dx a polyamnl (PA), ihin-fihn nmdxaine im
_bcn cxpc~d to, -an. oxidzin                  mcch as dikaine, hwnhre, or incfume        lne teat dftMihws qpalibtvcy wbcdior buIms have becme peat of tMe m.ua wlimauc                     tume A Fujiwwa test was moan a uw            ewsmpL. and itestrl    mwa      sitivm Ine ifedd spina b a plbstk vountain (Vema) dmjaW4 IDsuamtc mmanlma I==ve to him a ipow, jutih aod ;Dojmimafttuhla within kiodwateri pmsgesm Tie feed qm=a PWeafepacrga
'Mnpesmesteu            (riat         'ft'd a. pat for pamlem f [a* to mituiinzt pamcai-swi IEmm. 6              Thesspa= vms in     ma c~ -Z~ltctiu~ii   wfth no signs of daenmicz Gluelias:
Maidain leav= eam glued            6m     tAfu is to scjpmfte feed -anapcmeac $ft&,= Glue Uins dxauaob sousof ha-.
samples
              =I #=           w tea:                 lest Apitus 10 dtmine              =mbm The              cow    spa
                         *tbhe*
is opemd as                vab*, w te sat lx        g a0r= st eqmessed; by ai *B' valuse. &eA caxuftazi ate hxfiioss Of fti paapa*s of tne              ommaelds MnY hmghyevr Ne mind todis&solved Solid Of fth ftcszUnM.          owl value units we almsecl A Vistu Tr4noiogfei. far-
                                                                                      -page4;7fi)
                                                                                                    \3Ql

08188002 AVaie                B Value'"
Serial # A8141661                                      1.04E-    ".51E-5 Mamzktumes,oo       ig iinal                      8.00E-4 to 1.08&4     5.50 to 7.44E-6 FOULANT ANALYSIS
.Lessojiignioen:
Lows on ignition givps an W             -md  cf fie ora ic ~oftdoIfit=an            Value in.
exe of about 3% represent a sinilicantl o1rgai cmutw LOWs on ignitioc was 503 Mw awe fozdadem,*d          :
Mmixane; *za= density is Ie. Weight of' dty Wcuant Per am of menixmne unIfcc.
Foul-zt densities deterined f          past            rane fro    0.04 to 06*
* and Bubble test, Sevral&#xfd; dreps of dilufte hypkdro      qoiacid weeplacedn bc,   di    ulaut =&=ice     Bu~bbles indicate the presnce of carbonates. No bubties.evolved.
EDX,'sEMwsl          "is EDX. mlisi is coubeuted im omuctqwco ith        ma      rmu electron km ww~ (SMe to woflem mmsak:   mdm    he     aia-bipaidcorponntsofdie fidant vi    alunfinD=    s~dk=0o~pmL F.R analysis.                  organic fliest         "Ainant 'Fatty acids were detected Qiate0.y             un, compunmdswepe iununot deoalt pefomeiL A trace amount (1-94B4 96) wa's detcted.
A Aublntunmul was stained &#xfd;amd etamirrd. wit a lig            mnkch   pe. Signifiicnt umnb~cm otrud--dshapodiactenu (lrclh)we wn          seen.
COMPATlDILITY TE A amqt~i~-test -wm's z between ffielS~pechrus CMMl.0 bicide- and now Filnnec
.BW30.mmnhone Tesis woivre r -m       in a celts paaisand in a total recycle mode. The' results am graphed~ below Ai~slaTcinimpke. Inc.
Page 5-f1

                                                                           ;UJ     15 bUU0 Spectnas CT1300-BW30 Compatibility 9.OOE-05 6.OOE-05.
57.OOE-0    .*                   1                 2               3 400E-.05 ::::'...
6 04.OOE-)5,      -
c3.OOE           2.OIE-05 1.00E-05       ..                     .
2             3.                4             5                   6 Time, Hours.
1-4-AValues        --     BValues I Atpin w,01    p     o   petu C.30            ..       ...~w   pmws       de      a     ontto and a ttal of4 pm was added'at pointtee.
ISUAMMAYAND CONCLUSIONS A wet tes to dtuin *ment                      Adta c,. ould         be cm&=& Howe=,,                  cell toos of a, manb=ar BROVI shed pemwal flwr at the- high ax! of 'wxWl                                  :&sect;alt Claiugte RO sysema hk the *rewu cfreictkn sazniln cm=lochsea a inl the fibrglass wmuppA& ,!as e= in fthiekinelt However,, it was reported tht his gicdewas never mpblyecL Nog diffoiet prmac cmd'by failin may alao result in fthtype of danmge Ft:*,wvam.t   waypoi: v. fw                       mye CXdm.            elvated W&
am!pecfate flow at the hig" cod of uma         tB M1w fuanut cnints of claya polbr'w,          sc alumiummihyddel, ar                   bww         and ba4lrisl sliuw A U=ac Of qUaemMYary mmzMiu COMPcumd wasdetwctedby-a wet test jincedure.
Nie. was: detecedbdy FTuR analysi           11w:                 &lliet id 4wtzatnW amirxs by, FMR does mtnebessarl MM'"         tht &hqwine abscut. It uuy,-',     tiyn        fnthathey w% blo auqtest               Ihwdta SpectrusCF30fusFhecB-                              nnla.
Flo*w de&clnd whnt           itas 0.1 MM Of bioCile Was added to fthc=ll teat apzartu A.
tota  A value declie of 26pcatwas seen after. theaddito of 4"ppm Spectrus CIrI300.,                                                                .         .. '.. /
Avista Trecsnofoqgs. Inc.
                                                                                            'Pagi6 if II:
l
                                                                                                           . 0

03118 8100 2 pmow"ccd in a cuntmmks mode, of testng Inza iuyd mode, fte snal qfiankis of, the test subM=nc presen.i thetest IoT, may be&#xfd; adsolibed onto the meafmbi           surfce wVI~out ccwcnfg al, o~f the potestia atthncl='tsihes In A welinuous mode, of qtezation,
    'i                 s in.padded to the                      uous all of the membrane atahen ie wifl eventull be covered, and a: much greater.degro of, Ibidig i o~wo       tW rao~ns6 w      our testing was coucitd* in thei nzycle mode.
A cleaning t, was pedripod cm, a bed nnmw smnoml Expclet cleanian-resubl, wese obtaned with Avis's Ro~lan Pil dclaner., Howcve swtkesg did iees IRECOMMENDATIONS lbe SpectusC 1300 finals F&MpecBW30.mcmbrwce. We recomoamiez tatadiflinet   l IAniialeumc     Pilu T,         sa             ri.ki,.t atimaybe a ispoxluct wf*l Aviuta RoClean Pllt cleaner shuld, be. evaliwatrx        Vt May be a mot      cost-efibtive PW=uc for cleaning, toe Sysytem*= do,acid and caus&z curently wsed.
Pliw ded~ ionaprocedure Awould be Tmkvieed Avisa Tv'n "'S -'.'nc Tcg;r7qf     Ici 119

0 t3188 02, APPENDIX A Spiral Wound Membrane Consction I laxIamm!
                                  'aMINAUM WONODU A'i.,mi Technbiuoiref. Inc.
                                                                   '50~

0,31 8o8 0 02 UiPENDWI FgApre 1 Defoiuwecoing:
F!grn 2 Fouled membrane leu~f p., j

0318 8 0 0 2 Fgujre3 Fou~afmtrface
                                 .7r f
7t A*'a 4 Stindfuln    skoi'      ,i     r 4

03 11880 02 fi-are5 Cleaning .resul
                            , r -if 1i!
573

APPENDIX 7
           ' 1 f14

H H CHEMICALS,                                             !NC.
                                 $00 SOUTH VERMONTSTREET                                  PALATINE, ILLINOIS 60067 847/35847400                                                FAX NO.. 8471358-7082 DATE:'            Auigust 28,-2007 TO:              Tom, Armon FROM:            H. Ai Becker

==SUBJECT:==
indianaand Micdhigan Power-Company Donald C. Cook. Nuclear'Plant Mexel (A432) Efficiency Study Compiled Analytical Data August 2006 through August 2007 D~ear Tomt::
Attached please our laboratory analysis .reporton the above referenced project. This report is. a,year long conipilati6n of laboratory and field' analyses 'on water, deposit; and corrosion coupon samples. In addition&#xfd; thisxreport'i'ncludes the:membrane autopsy data from. two sets of fouled reverse osmosis membranes.
.1ihope this inforniation0satisfies your requirements. If any further work ordiscussion is needed, please get back to' me.
Very truly yours, H. A. Becker HAB:ld Enclosure

LABORATORY REPORT - WATER ANALYSIS                                                                  Customer No.:                       1001392 H-O-H Chemicals, Inc. rRegarding: Indiana and Michigan Power*Company                                                                       Report No.:                 as indicated 500 S. Vermont St Location:, Donald C. Cook Nuclear Plant                                                                           Report Date:
Palatine, IL 60067                  1. Cook Place                                                                                 Analysis Date:                          I' Bridgmah, MI                                                                                  Sample Date:                as indicated 8471356-7400 Fax: 847i358-7082         Control 9/7/066
                                                                               .              Treated.917106              ;Conrol19114/066              Treated 9/i4/06                       Control 9/21/06
(#26723)                      (#26723)                      (#26743)                        (#26743)-                          (#2678)I hnIi~eII 1,i            1,dh          1              I 1n.Iha .h       I I-WIMhI~        QrIht.h.         I I-hffif. I   QMI,hl.        1-~hihi.,
___                          ___                  -,..t-.,..1~
Alkalinity ("P')         __          as Caco 3                                          12                                                                 4                                0 1.20                         1,*28                                                                                      .........
118 2: Alkallnlty' (,'M)           ___         as CaCO 3 &#xfd;                                                                     132
:w           Free Mineral Aci!*ty.               as CaCO a          Chemica Oxygen Demand (COD.)                                                                                         4:9 t   5. Chloroform Extractables                                                                                                                                    128
: 7. Dissolved Solids                                                                          198                           198                                                              .191 r          Hardness (Calcium)                  as Caco.                                           74                            .77                                77 Hardness                              s CaCO 3 iMgeim                                                                                                                                              &#xfd;43 115                           120                                                               123 8.5                           8.4                                                               8.0 0          Specific Conductance              ~mo                   olop                           298                                                                                              298 p          Spec!ric Gravity                     g/m     .
            ,S             Solids jpended                                                       10.0                          7.0 r                                                                                                           .0.06                                      8.2o Aluminum,,                           as Al              0.0                                                         0,01            0.69                             0014             0.01           0:55 0.01                                                               42,02   ..............
0.014 Barium                                sBas                             &#xfd;0.01                        0.01                                                                              0.02           0.01 Calcium                              as Ca,                                                                                         5.33            30.7             1.28             3186           7.20 1-.32         29.6           0.48            30.9 e          Chromium                             as Cr                                           0.0-00                         0.00            0.01                                              &#xfd;0.00
                                                                                   &#xfd;0.00                                                                       '0.00 0.020 Copper                               as Cu              0,00                                                        0.00                                             0.00             0.00 S0.00 Iron.                                asFe               0.00
                                                                -----                                           0:06            0.00                                             0.20                            0.01 001
: 21.                                                                          0.000          0.00(                                                                         .0.000 Lead                                 as Pb                                           0.00        0.000           0.000                                                                0.00 0.000           0.00                                                         0.02 0.,95*P
: 17. Lithium                                 asIl                                                           0.00                                            0.,00            0.00             10.60         0:002
    %1.                                                                                                                         0).00 10;0     ........:0000 OMOO
: 18. Maanganese                              as M                              0.3E          0.00           0.00 1.00            0.28
: 24.                                                            0.00                                                                                                                          1&#xfd;08
                                                                                                               '0.00                            0..
6600....
0.01                             001%                            0.00 0.00                                         0.00
: 25. Nickel                                  as NI.                                                                                                         .00                               0.00 0.00 1.24                          1.30                                                          :1.57            0.0i 320. Potassium                              as K 0.00 6:
                                                                                                                                .1.25     ...... 47...q         0.00 0.01
                                                                                .............. 9693                                                                            0.00             0.00 C          Silve Sodiumr     _as- .......           :asi Af ......
Na 6,24
                                                                                                 ,0.00          0.00.
66              .812
                                                                                                                                                         .........   .......                      61.2 a                                                                                                0108
:t :.22c Strontium                            as Sr 0.00 0 00           06.00 0.......
00i*                        0.11                                                       ....
t,             0.01 Zinc                                 as Zn                             003                                          0.00                            6.51             0.03 0.05                        26....01*o 0   23. total CationMilliequivalents                             2.607                                                      2.687                   . .......... 0.o0                          Z.755 0.10                          0.00::
6 33, Acetate                         _    'as C2H302 0T:.00                                                                                          0.00
_S                                                                  &#xfd;0.00 Bromide                              as Br                                                                           10 0.00 Chlorate.                            as C103                                                                        0.00                                                              0:00
    &#xfd;36, Chromate                                                                                                                                                                         ..................
                                                                                                                                                                                                        .18
                                                -as Cr0 4 ___       0.02                                                                                        .0.1
: 27. Fluoride                                as F&#xfd;                                        1 0.0-0                         .O11...
: 0.                           ..:
FormateasCO                                             0.00                                                        0.00 0.0=
                                                                                              )   0 00                          0.07 0:0p
: 41. Mpfybpdate                              as MoO4            0.73                                                        0.80                             0.01 Nitrate   _              ___         as N03,                                          0.00                                                           0,08                          .....
o . ..
0.00 Nitrite,                             asNO 0.101
                                                                                                                            ------                               Ooo                                 00 Nitrgen (oaol).                       as N .             0.00                  K.........
0:.00                                                                                            21...
                                                                                                                                                                                                 .7........6 o
Oxalate-...                .        .as
                                                ' C2 4. .                                         0.00                                                                                            0100
                                                                                                                                                                                               . 6*83.
0.00 Phosphate.(ortho)                    as P 4                                           0.00                          0.00 0.81 Phospate (oly)                  as P.04 _                                                                                                                                      3..1!82
                                                                                                                                                                                                --0.00 28.. Phosphate .(qrgano)                    as P 4 0.00 hsporu (QotAl)                      sP-                                                          0.10                             017              22.1                             0-00          .0.09 Su.lf~at~e ______                     as&sect;$04,                                                                       &#xfd;21.6                                                                                   .
529 Total Anion Millefquivalents Ammonia                   _            5NH 3.2 18 0.95          0.25            6.79 4.119 2.12 .I
                                                                                                                                                                            ..     ..o 1.02           0:82 53.
Benzotriazole,                        as CqH5N3
: 55. Boron                                   as B               0.80                                             8          o.oo                                             0..0....-
0 silica                                as S102                           1.07                                                         0 00                                                             &#xfd;245 5o. Sodium Nitrite                          as NaNOZ           0.83 578.TSodiuultazfite                        as Na2S"3 5.Tolvltriazota;                          as CH2N,
         . Au GSS&#xa3;ZC.prpSI,,,pans perWithaloresm&c*WO                                                uontjnueo on reverse sios.
Afid=-WpM.p.Mprftb....&#xfd;-td                                                                 Continued~on reverse sice.

LABORATORY REPORT - WATER ANALYSIS                                                  Customer No':                        1001392 H.O-H Chemicalsi Inc. Regarding: Indiana and Michigan Power Company ,                                                  Report No.::
jReport'Date:                   as indicated 500S. VermontSt. Location: Donald C. Cook'Nuclear. Plant Palatlne, IL60067                 1.CooklPlace                    .                                              !Analysis Date:                     -
Bridgrnan, MI                                                                  Isample S            Date:           as indicated F~ax: 8*47/358-7400 847/358-7082 Contrl19f7/05            Treated 9/7/06             Control 9/14/06         Treated 9/1'/06                    Control:9/21/06
(#26723)                (#26723),                   (#267,43)                 .(#26743)                          (#26781)i Soluble     insoluble I -soluble       Insoluble      ,Soluble  Insoluble    Soluble         in'nolujble.      Soluble        Insoluble
                             .                            . ....         - ...         . ..- i........-
                                                                                                     -  -.. ,,,---.. ..    .. ...._..._   ....                  .... 1...
Bromate                         as BrO 3  _
1C 64:      chlorite                        as:CIo 2 0          Cyctohexylamine,
:m          Diethylarnine*                  as C4H-11N 0     671, Diethylaminoethinol'            ats CalHiiN0 u          Ethylahiiine*-.                 as C2H7 N
: 69.                                   &#xfd;as CHq1N0 EtthyleneGlycol-        .       %byyeight 6.s 72.
                                             %~byweight
       -73. P~ropyl ene Glycol`
Aerobic.Plate 'C6unt M     65.
79.
Fecal Coliform                  org'slmI C          Iron BBacteria                  org !smi                              I................
r          Mold                             og's/1-11mI 0
b          Nyt      Reduceari-                                                         000 iii~i~
Slimhe Forzme',s'               -org'smI 0                Sulat Rducers org;'s/mt0rn otlColiform, 70.
781 7...:*ResiduepybyEvpoqration 808 Volatlle-Solids
-'a          .System_~Capacit 0.00 ota!l Organic .Carlbon_                           2.10                                                                          2.50 Tota OrqancONitrogenP                                                                                     029                 ;<0.124 3.0                   .........
      ,a    Mdatmn-&#xfd;     Him   ft  mOm     kdkW&#xfd;

LABORATORY REPORT -MWATER ANALYSIS                                                                                                   Customer No.:                              1001392 H.O.H Chemicals, Inc. Regarding: Indianaand Michigan Power Company                                                                                                                              Report No::                      as indicated 500 S. Vermont St ILocation: Donald C. Cook Nuclear Plant.                                                                                                                                 Report Date:
Palatine, IL60067                                            1 Cook:Place                                                                                                                Analysis Date:,
:Beidgman, MI                                                                                                                 Sample Date:                    as indicated 847/358-7400 Fax: 8647/358-7082                        Treated 9/21/06                            Control9/28/06:                           Treated 9/28/06                          Control 10/5/06                         Treated: 10/5/06
(#26781)                                   (#26813)'                                (#26813)                                 (#26859)                                 (#26859).
Soluble         I     Insoluble           Soluble.              Insoluble I          Soluble,           Insoluble I            oluble         J. Insoluble            Soluble        I Insoluble
: 1. Alkalinity("P!')                                   asCaCO                    "           0                                          6                                         6                                         0                                      0
       .. 2.Alkalinity,('i ..                                ascac 3                "          11 .112                                                                             114                           .           114             _1*14 3....... n...(.OH.).............s...C...
WY 4. FreeMinerlcdt                                          as CaCO____
a      5. Chemical_O ygen Dema*nd (COD.)                                                        4.A4                                        6.4 .                       .            9.6                   .              ..      6                                 12.7 1 6. Chloroform Extractables e-7~.Dissolved-Solids                                                                          193                                        195 ..                                    196                                      199                                        620 r Hardniess&#xfd;
             .:               (Calcium)                      a~sCCOw __                                                                     880                                         0'                                     7                                         6
                                       .HadesCc4444                                                                                                                                  44                                        43                                      43.
(Mag--s-u-)--s-C-.........                                    ...........                 .........................
                                                                                                                                            -.-H ......                 8...                                                                       ..       .                   ....
P '10. Hardness (Total)-                                    -as CaCO 3                         12                                         125 .......                               125                                      122                                    122
        -_ -_   -                                                                 81                                        82-----                                     8.2   _                                 8_                                      8.1 o    12. Specific Conductance                             *mhos                             285       _                  .             290                                      295                                       294                                    303 p    13.. SpecificGravity.                                    m......                                                       ..... . ..
e    14. Suspended Solids                                                                                                                                 470                                         80;                                     15.5                                 A20
: 15. Aluminum                                           sAl.
a_                                                   0                                                                                                                                   5001          0.01              016
: 16. Barium                          _                   asBa                             0.02                0.00                   0.02                0.01                                       0000                  w002                                 0.02               0.00 I17,Calcium              ___                           as Ca,__                         31.8                0.00                   32.2                6.04          _32.1                        0.02-              31.3                 0.00                313                000 e, 18. Chromium                                              as Cr.                           0.01                '_0.00 0,01                                0.00                  0.01 1_                    0.00 _                  0               00                  0.00
                                                                                                                                                                                                                                                                     .0 20 jq        opper                                         as Cu                  __0.00                        0.01                   0:00                0.01                  0.00                 0'.00    __        00                      0       __0.00                     6_.00 iron               ..            _               as Fe                  _         00                  0.0                    0.00_                1.04'                0.00                018                 0.00           0--6.30                   0.0               0.2
    *21. Lead
: 22. Lithium.
                                            ....... ..sM     as Pb .
as Li
                                                                      ,............... ....: 1..............
0.000 0.00
                                                                                                              .. 000 0.00 0.00 ....
0.00 0.000 0.00 0.000 0;00 0.000 0.00 0.000 000
_000 0.000 0.000
:000.0, 0.000 000
: 23. Magnesium                                           as Mg                             M08                0.00~                  10.7                20                  4.j                     .6                16                    0.17               10.5              000D
: 4. M              nese                                 as Mn                            00.06               0.00                   0a00                                      0.00                 0.01               0.00                 0                    000 001               001
: 25. Nickel                                              as NI            .               0.00                0.00                   0800                000                   0.00                 0.006              00                      0                000 2.4 Potassiumt                                          as K                              1.39                                      140                                       1.86                                     1.22                                    1.42 274.Silver... S~ t.t.....as    ~~~ ~ ~ ~ ~ ~33...d..*..B.0O.0..0..OO A0,i                  ~~~~~~. &deg;000             00                          0              &deg;'000                 0,00                 0.00              000                    0o00
                                                                                                                                                                                                                                                    ..              000 ...... :,.,* 00
                                                                                                                                                                                                                                                                   ,*0 C 26. Sodium                     __           *...
                                                -."C   ofi. Bas
                                                  ...h...          ~
Na ...
a**s.........
                                                                        . . ..... ............643 11                     ...... .. .................. ... ... ... ......... ... .... . 7,00
_6.49                                     _       _                      .....................
                                                                                                                                                                                                      ............ .........61
                                                                                                                                                                                                                             .. ..765 .. .....1...12..1......
a2. Strontium                                           asSr,011                                             0:00                   0.11                001                   0.11                 0.00 --            0;11                  0.00               01*1              0-00 2 784 ...............
310.: Zinc                                                   Zn,                         0,01                0,04                                       001                   000                  0 01               0&#xfd;01                  0.01               0&#xfd;011_            001
                     ~         .yo
__as TotalCatiornMillequivalents M.                                               ------------  - ----.....
                                                                                        . 2,798
_0.01 2 810                                     2' 40                                     2 734  o...o_-_-        __
Acetate
::k32                                              as 2       ~     2               0100                _            _         0.00                                      OJO00                                   0,00                                     0_00 5      17..-
: 33. N1-r-.t........... .. . . ...........
Bromlid&#xfd;                                           -*
6s'Br N 2-
                                                                  -----                          M00............                         oo 0700                _                    0.00o :       "o                         0.00 o                     ~oJ            000 ............
534 Clotriaoe                                           asC             N                115312,2                                                                                                                     115
: 35. Chlorat                                             as CB5                           0.00                                       01.3                                      0.00                                    000                                      0,00
: 36. Chrmate __ A 4B. :as                      Pfiospliate PO*..........   '(poly) as Cr0 4__
: 37. Fluoride,                                           asF-0L0OP0600                                                                                                                                                                                            0
: 38. Formate_                                           :as CH0 2             _           0,00                                       0.0                                      00000 M                                                                             0:00 3.Glycolate n_5
                   ._r.(Lo
:.su.!u.t~..!)............
                                     .          _              s 2 5 3                        0000 ......................................... . ...................
                                                                                    ................                                                         ___                  0000_ ...                                                     _000 40Mlybdat                                          as!oO4                           00M0O___                                                                               0                                        00           _                          0
: 41. 52 Nitrate Am    m on~~~~~~~...         .......              asN03                          .081                           !-.. ...-     0;75 . ...------..-_              . .....0.79                                      0;92                              ... 0688.
: 42. Nitrite                                             as        N2                     04                                                             000                                                                                                    0.00
    '43. Nitrogen (totial                                    as N--                                                                                                                         -r--  ---   ----
: 44. Oxalate:                                            as C- 4                          000000                                                           00                   )0000_                                                                          0.00 A46. Phosphate  SS:Siicaas   j(poly                         asP0 Si~  4.
_- .,       _ 0..8                  i0._0Z -_. /.:_*4                            3; 5           ... ,_ .80 ........... ,....8....2                                      _0_.........0"           .............-
n  .A.&#xfd;asjO                                                                                                           -                                                                                                                                    ---
48 P4osphorus;(total)..............0                                                                        0.03                   000                 004                   0.00                 0.00                0:02                 0.00               0.01              6000 0    49. Sulfate6                                       __as S0 4                             21.8                                       28                                        23&#xfd;3                                    225        _3.3
_pc5.,Sulfur (total)                                   As S                             6.90                0,00                   60                    11                  7.18                 000                680                   0.00              .6.81 .            0.00
: 51. ToaAhq_              a         _l t         _ae                                                                                                                                                                                                  .4
: 52. Anion.               equvaen-
                                              .ta.                                        3.185                                      3.094                               ---. 3_175 -_      -----                        3.122         _                               4 5.Ammonia                         _                  as NH3                                                                                                                            ___
: 53. Benzotriazole                         ~,            as C0 HsN3                                                                                    ._._...._.                       ....
54Bron                                              asB00160-95                                                                                                                               -                    01;0000
: 55. silica,                                             asiO                             0.88                0.67      __           0;94                365                 '0.60                  1.24                0189                 2.07               0:768             120 1
: 56. Sodium Nitrite                                         s NaNO 2 1 ci__
: 57. Sod-iium Sulifite _                                 as NaS6 3                                                                                  _
5S.Tolyltriazole                                        as CAHNs' F______________                                                                                  ...._.__._....._
                                  -        M~e5pose5~tadContinued                                                                                                on reverse side.,

LABORATORY REPORT - WATERANALYSIS                                                       [Customer Nol:                   1001392 H-O.H Chemicals, Inc. Regaid1n4: Indiana and Michigan Power CompanyV                                                             rReport No.:              as'indicated 500S. Vermont St. Location: L)onald C. Cook Nuclear Plant.                                                                    I Repbrt:Date:
Palatine, IL600671                        A Cook Place                                                                        Analysis Date:.
Bridgman, MI                          ..                                            Sample Date: !as indicated 847/358-7400 Fax: 847/358-7082              Treated 9/21/06               Control 9/28/06            Treated 9/28/06              Control 10/5/06        Treated 10/5/06
(#26781)                      (#26813)                  (#26813)                     (#26859.)                    (#26859)'
Soluble       Insoluble      ,Soluble   I  Insoluhle     Sohlhle      In-nluhln      Solubl. 1-nh6lbl                    Innoluhle; Insoluble Bromate        -.              as Br03 c   59.
60.
61:.
0       c!*hloaitoe tycloh~kyarmine'
_t__a 1._..       as&#xfd;CH 13 N DlehYlamine! -- ---------     -asC4H11 N, p        Diethylaminoethanol*            asC6 H15 NO
: 05.                                      aS C2 H7N u
n        Morpholine'                     as C4 H5NO DiethyleneGlycol'               %bywelghi PErtYlaene*:Glycgi  ......
69;                                  % by weight Aerobic Plate Count        .....org .s/mi ......
70.
M         Anaerobic Plate Count          .org s/rmI!
78.
76-
!C        Iron Bacteria Mold                            org'sIml*
0 r; 75.
Slime Forme..s 71.
0
: 77. Sulfate-Reducers_               org's/ml
: 72. Total Coliform,                 ga6lr       ......
O
-. 78.
73 Residue. by vprto volatile Solids.
a.
P~ropioflato                   &#xfd;as CH'                   0.00                         -0:00                                                                               o:00 2.4                                                                                  0-.0-0 Total Oranic Ca@rbon                                                                    :1.0                     7:62                                                     3.50 T8tal Organic Nitrogen                                                                                                                        3.0 Mexel :(A-.432):
                                                                                                '1.47 3.0                                                      5.0
                                                                                                                                                                                 ,^ . ..._
1.4-------
1.0
______      4 _______     fl _______      L _______    t _______     I.           I. _______    L ______    &#xa3;

LABORATORY REPORT- WATER ANALYSIS                                                                              Icustomer No.:                              1001392 H-O-H Chemicals, Inc.                         Regarding: Indiana and Michigan Power Company                                                                            Report'No.:                      as indicated
:500 S. Vermont.St. Location: Donald C. Cook Nuclear Plant                                                                                                        Report Date:
Palatine, IL60067                                  I Cook Place                                                                                               Analysis Date:
Bridgman; Mi.                                                                                              Sample Date:                    as indicated Fax: 847/358-7082                      Control 10/12/06                Treated 10/12/06                         Control 10/19/06               Treated 10/19/06                    Control 10/26/06
(#26908)&#xfd;                              (#26908)                           (#26957)                      (#26957)                              (#27021)
                                                                                                                                                                         .1.      _    _____          ____;
Soluble I Insoluble              Soluble:' I Insoluble                   Soluble- IInsoluble.         . Soluble, I Insoluble               Soluble I Insoluble
: 1. Alkalinity(P"):Pas.CaCO                                   3                   6                                       6                               10                                4                                     8 2.Akalinity CV")                             scacOi                         120                             .         20 ____                         120                            110                                   114 3 Akalinity ("OHW)(eelculseu                as CaCO3                                                                                                                                                . . . ...
a4.         Free MinefalAcidity:                    ascc1                                                                  .Ca6
_H. ........ ..
:i ;..............                . ........................
                                                                          ......                  ..... 2
                                                                                                 .:                        ......   .......             2.                                                                *1 Hardne"-ss (*Mag'nesiunm) . 'a-9.:*,-                                       -s-aCO3; ".....                   4&deg;3                                   . 42.......... . . ..
4 _..__,_.............     .. 44                               44                                   41.......
I.............. 41   ... . -!
1., Specirficm a 1i0. HardnessG (Total) xravity...a               ri. 3 asCacO                  a:       119[           _                        117                      82      122,                             122          _    __    85_     __       511             .
o12. Specific Conductance                      (.tmhos                           294                                                                                                       299                                 296 .295 3289
    ,e 7. Siss nd.ed Solids                         a...
                                                      ._ a     ........                             8010                 .          .         27.0              1              70..o                           18o                                       15:2 r     1.. Cal*carI--um. 1.Au iu                   aA000 as     CaB                       3050           001 85              _0700      .0 0.0       0.0200.000A0 4.22        __7 31       _      0;00            000 33                00.4                2                    00.....01(*........:'
14
: 24. Manganese                                 as Mn                          '004100 43                         _000_         42              .2             00              .0             0.0_00                                  .0                00 o 16. Chromium*                                    as Cr                           0.00           0:01                    0.00              0.01             0,00   _      0.00             0.01             0.00                000O                 0.00 r     20. Iron 288oimaa81           .as                             Fe:                           4 0.0...                 ............31 0216 1...........I40                          .4* 7 8.97 0.......             00                ........ 000 0......1        7.29     _6      022.... 8.09         0,00.... .3...      *0;B..1*
Totalfi CatondMluctancent
: 31.                                                                    2680o           2                        858                                2891___                       2990                                  2.59 V_34. Shlr&#xfd;d                                     asC                              11812512.                                                                            ____                   2.911.
     *25. Nickel                   __                a I0.00                                        0.00                    0.00         _    0;00            "0.00i         0.00        _    0.00             0.00                 0.000               0.0 P26. Potassium                                 as KCC                          1.38                                   1.54                               1.45                           :1.68                                 125
: 27. Sierou                                     asrAg000                                       000                     0.0         -0.000.00              0.00          0.00             0.0              0.00..              0.00
: 29. Strn~tium                                  as. S                                                 .0               019.1..............0.12....00o.                                                                        0.10                 0 n.....2. Acetate                        __"        as C2H3O2                       0 00                                   0 00                          "0.00                               0.00                                  0.00 5 33: Bromide                      .       .      as Br                          :000 "                                  0:00                               0.00                           0.00               _         _        0.00                I
: 35. Chlorate           Soli                    as CO1                          000   _0,00                                                               0.00                           0.00                                  0.00 Chromate                   .36           as C .......................
4 as MO 0*
00.02 0.02                                   000.000 00                                 006 .......... ..              006                   .               008.......
0 *00 40.
3915. Bdat MAlucoint 37 loieasP
                       ...           .          . as     F AH                        0.06 0;00                                    .000 __ ......0 O;O_                              -0.00                                                                                     P
: 41. Nitrate                    _               as NO                           000             8                      0.95                            _ 077                               0490                                081
: 42. Nitrt*e....         ..    .                as NO2                          0.00                                   0.01 0.00                               0.01
                                                                                                                                                                 .           0.0              000           .                       000
: 43. NItrogen (total)                           as N: ..                                ..     ...                 . ..                  0                   .                                 0              2 44.
58    Lxaladte . ...
                                                    !as Sr 5N oo000 0
0111 000     0 oo000 ___1__
0000 0
oo                             o.000 12________0.10____
d;o M     Sytroniuml 4 . Phospha... tr.......
__            __0__                                   __01 _Q.12_____
46.
2 Lithiuhrmu(ta)                              as LI                           000            0;04                    000               0003        _000 .               001            0001'                3                oo0.00              ;000o 49, Sulfate.
0                                              as SO            .              221                                    237                                231                              238                                 207 Sulfur(total)                           :5.
as S                   ,          81           0!71                    6H92              0640             730           0,00               0.764          0.00                 7-13                 0;0C
: 51. TotaIAnion Millequiivalents                                             -3218            -                     3291        _      _                3J274                          3.308      _        _                3;088 ......             .......
: 53. Benz~otriazole                            .as C5 H5 N3                                                                        "i
: 5. Boron                                     as B                            0       0          ;00                 00                                 0.000              0             000            0                     :.00               i 555. S lica.....
1 *ii                            as. $103__                      061............447
                                                                                            .......                         078               1.4454 0 80.88.....087...                0.91.....
91         018:08108                        0854                                      1.04!.0
: 58. Sodium Nirite                              as NaNO_                                                                                                                                                                                  ...
: 57. Sodium Sulfites                                   Na6SO:
Nas1                                                                                              S790 0,          T                           ui=,,               ,,..ontinued                                                          c*llpt          on reverse side.                                                               Z589

LABORATORY REPORT - WATER ANALYSIS                                                        iCustomerN&..:                     1001392 H-OH Chemicalsi Inc.                                  Indi~ne ~nd Mit-hint n Pn~,jer C'nmn~nv                                                 IPnnrt ?Jn *            . inldiri~atpiI Re    rdmj ndMihianP6mr   Idina               or       av.I                             e r-'' No:            s   ndc. e 500 S.,Vermont St. Location:                    Donald C. Cook Nuclear Plant                                                          IRepoot    Date:
Palatine, IL 60067                           1 Cook Place:                                                                         lAnalysis Date:
Bridaman. M['                                                                          ISamble Date:           as indicated 847/358-7400 Fax*. 847/358-7082                 Control 10/12/06         Treated 10/12/06               Control 10/19/06            T"eated 10/19/06             Control 10/26/606
(#26908)                  (#26908o)                       ,(#26957)        j          (#26957)                   (#27021)
RolhlhIA                                              I t~iti,,hIn   flnnnlihln I                  Insohtbln                I c,!
'0 Bromate6 Chlorite:
as Br0 3 as CdO2
                                                                                   ~
S~
Insolubie Inoul olule 5~nIuhIn Soul--
Insuittitin, 1-0e;I        Soule        I_____         Slbl nsl
                                                                                                                                                                                       .    ,nOlubnl       Insoluble Cycl.hexylamine                     .            as C6,H13 N m
p.
0 Diethylaminoethanol*                            as C H15NO U-    E~thylaiine.                                     as C2H7N
: n. Mo.rholine                                       as, C4HNO d      Diethylene Glycol"                                ,by weight S"
PrtyleneGlycod                                    % by weight Aerobic Plate Count.                              o.g's/m Anaerobic Plate count                            org's/ml c
Iron Bacteria 0     Tecai Coliform ,_                                org's/100~ml t
1i Yelasteeues                                      org's/mi
:.o C     Volatile Solids Total Col32ior                     -                rsOnI 0.........
0.00                           0:00                         .........
0:00o TI~ Ot:rganIc: carbon                                                    <1.00                       2.06                                                        0.485                     4.07
              ... ... .   . .......                                            :<.124                  . ....1.06                          0.55                                                4-0.50C Totl~ranl Niroen
                                                                                                        . _6 .0.                                              ...... .........
                                                                                                                                                                         *o
                             ..... ...   ..                               I S Audll d   nsa  t              rl ant nl it nnin   e
* tnduin                                                                                                                    AlavsS bN(&#xfd;s WaNsis   yGa ChmmdcaraDh, hmalaa

LABORATORY REPORT - WATER ANALYSIS                                                                                  ICustomer No.:                                    1001392 H-O-H Chemicals, Inc, Regarding: Indiana and'Michigan Power Company                                                                                                       Report No.:                        as.ihdicated 500 S. Vermont St. Location:- Donald C. Cook Nuclear Plant;                                                                                                        Report Date:
Palatine, IL60067                                   1 Cook Place                                                                                                 Analysis Date:
Bridgman', MI                                                                                                Sample Date:.                      as indicated 847/358-7400 Fax:-847/358-7082 Treated 10/26/06.                                      Control 1 1/2/06.             Treated 11/2/06                         Control 11/'9/06                             Treated 1149/06
(#27021)                           (#27043)                          (#27043)                              (#27107)                                    (#27107).
                                                                            ,*nhlhl*       J      Inonl= Ihl*    *h   shln       Ine*hht=      'P*.*IHhl&#xa2;         I  InenlHhlA                                                      QM'.61.       1    1-1"            1.
IQ luble I Inolbl     '~~            col     l    I_ Inolbl        'Slbl                 Islul
: 1. Alkalinit    ("P.)                           as .CaCO3                       6                                  0                                                                          S ..                                     6
_2. Alkalinity ("M")                              as caCO3        ............ lie                                1.34                               1216 Alkaliniy_(W'OH")(lc                  )     as ca...CO.
Free Mineral Acidity                        as CaCO    3
       -4.
9:1 a       5.; Chemical Oxygen Demand (COD.D)                                           7.5                               11.4                                                                       5.2                                       7.7
: 6. Chloroform Extractables:
a
: 7. Dissolved Solids:                                                                                            222                                                                      200
: 8. Hardness(TCalcium) 263 as CaCO3                     115 41                                  44                       ---- - 44                              881.44.                                            45 iO: !Lard ness (Total)                            a aO                                                           '125                                125                                                                  ......................
128 rp 7._8                                   8.4'
.0 12. SpecificConductance                               pmhos Specific Gravity                            g/riil
: 13.                                                                                                                                                                     -167                                        6.0                                        4.C Aluminum                                    as Al                                            0.05            .001                            0.01                0944                                       0:03               0:02                   &#xfd;0.011
,e           Baiuilm                               '-a-s B"a -                                           0.00           0.02 ..............
                                                                                                                                        ....              0.02                0.01 ...........32....              ....
                                                                                                                                                                                                                           .q
                                                                                                                                                                                                                        '0.00               0. 2
                                                                                                                                                                                                                                                                   --- 'o 18.
: 15. Calcium                                                                  0.001                                                                 32.1                                      3.
as Ca                      '29.5                '1.13          32.4                                                  10.1                                                          33.2                   0&#xfd;00o 0.00 Chromium'. ..
             .........                                   as cr                               ....         o..oo         0.00                              0.00                6.00              0.00                                        0:00    ............ o copper-6.......                             as cu                       0.00                               0.00           501.012..................
0.02               60 00 6               0.00 i ..... .............
0.00,            ........
                                                                                                                                                                                                                            .o
                                                                                                                                                                                                                       .__0.00 0.00 0.00                                 0 00                     0.00                                        0 00 20.. Iron                                         asaFe                      0 00                  0-12          0.00                                                  1.67                                       01*6 0.000
     '21. Lead,                                          asaPb                                         0.001 ...........
0001            0.001            '0.002               0-000 ........'0.000 .......  .               0.00                                        0 00 S0.00 Lithium                                     as LI                     0.000                                               0.00
: 22. Magnsm. .......         ..                       Mg.                                         0.*00                                                               0.00                                                          0.00                     6.00 233.                                                                        10:00                   0:23                         179.                10.7              .3:30              10.7                     0.00                        ................ .,....0 0.00 Manganese                                   as Mn.                    :0.00                                0.00           0.30                            .........0_.o 0.00 ............                    0.00 *___                0.00.              0,00 0.00          '0.00           o....
oo3.                                   o                               :
Niclkel                                     as Ni                                                                                  , ..._:*....... .
: 24.                                                                          0.00                  0:00 1.33                                                                  1.66                                  1.39                                                                0.00 Potassium,                                 asK,                                                            1,77 1060                                                                                                      ....      1.56 L~
327.                                                                          0:00                                0.00                               0.00               0.100             0.00
     .25B                                                                                                                                                  8 23                                 8.95 Sodium,                                    as Na                      -..15                                0 00                                                                                                                7.30 C. 30.
               *i* - * .. .................
: 29. Strontium,
                                             -------  &#xfd;as Sr
                                                                    -                0.10                 0.00
                                                                                                     .. 0 01.."
0.111          o.03                0.11               0:00 0;11
                                                                                                                                                                                              ,0.01 0,00               0:111                   ,0.00
                                                                                                                                                                                                                                                                   .0.13
     '25. Zinc                                       6as Z                                                           0.01         . 0 04                0.01 28.
i32.
Total Cation Millequivalents                                        2.600                              2.903                              2.892        -:---------
                                                                                                                                                                           -*:               2.842                                     2.921.
0.00 n            Acetate'                                   as C2 H302                  0.00                                0.00                               0.00                                 0.00 5:           Bromide                                    as Br                                                                                              0.00                                                                             0.00 34- Chlodde                                       as Ci                                                                                              14.0                                  12.4                                       12.4
: 35. Chlorate            _            _            as CIO 3                                                                                           0.00                                 0.00                                        0.00
      ,35.
chromate                                   as crO .
0.05
: 37. Fluoride                                      as F 0.00                               ,0.06                            .0.05                                   0.06                                        0.05 Formate                                    as CHO 2                                                                                         .0.00                                                                              0.00 0.00                                 0.00 Glyclat ___~~~ as c6H:o                     2                                                     0.00                                                                                                                 0.00 M01ybdate                                       MoO as__Moa4_     .             0:00                               0.00 0.00
                                                                                                                                              . ._......o...Ooc                                 0............
0-                                      0.00 0".91 41_ Nitrate                                      "as NO3                                                          1.20                               1"09                                  1.05 42: Nitrite                                       as NO 2                                                                                            0.00
                                                                                                                                                                                                          ............ "6                   0.00 0.00
                                                                                                                      .0.00 Oxalate,                                   as C:O 4                    0;00,                              0:00                                                                     0.00 0.00                                                                             -0,90 0.00
      '45.
: 43. Phosp-ate (                6)      -rt--- as P0 4 .                       0:00                        ............
46., Phosphate (pal)oasjP0 qL ........                                                                          '0.00 A                                                               4
: 47. Phosphate. (organo).                      " as P0 4 hosphorus (tota)                        as P,            ............21..4               0.....
                                                                                                           ..00                                            0.00               0.46                                                    .........
0.00 0           s.
Sulfate~:
                .!~.............      ............. .... . sS .....
asS     .4 ...                                  .0.00          24.5                             .24.6                                   22.9                                        22.4 n     soo    Sulfur (total)_as                               S 813e                             . 7.. 4......
7;                   0_00                                        0 00 7.08                               8.31            0727                                                      0.00                                       7:63 a
50t   Total Anilbn Milleqluivalenti                                                                                                                                                 ....... 6?                                                                 0.0 52.,  Ammonia __                _                 as NHi                                                                                                                000.''*,*                                                                    ......'... c'3
: 53. Benzotriazole ,                           'as CHN                                                                                                                                  008o                                               ........... ...
: 54.           ..............
                                                  .....a. ......... . ..            0.00                               3180                                2 29                                 0:
Y'" 00..... .........                      0.00
: 55. Silca                                       as S1o2
                                                                              .......... l6                                                                                                                                                *1.06 05                          14.41                 1.16                                                                                              .0.31
: 56. Sodium Nitrite                                as, NaNO_
Sodium Sulfite                              as Na2 SO3 58,   Tolvltriazole                               as:CH ,N IAOdatWocpt pHInpaft pwM11onw,a. lda~d4                                                                          Continued on reverse side:

LABORATORY REPORT.- WATERANALYSIS                                               Customer No.:              ,1001392 H-o-HIChemlcals; Inc. Regarding: ndiana and Michigan Power Company                                                    I Renort No          as indicated 500jS. Vermont St I                       n^mmiri r&#xfd; P-L-Al     ^IA-     Dlnri+&#xfd;                                    Dm ^rf fl&#xfd;+
n                                                                             I E          -
Palatine, IL60067                        1 Cook Place                                                            lAnalvsisDate:
Bridlgman;.MI                                                - ___sample           Date:     as.indicated 847/358-7400 Fax: 8471358-7082 Treated 10126/06                    Control 11,/2/06        Treated 11/2/06           Control 1119106         Tr*eated 11/9/06
(#27021)                (#27043)                  (#27043)                 (#27107)                (#271 07)'
                                                              .Soluble  I Insoluble   Soluble,   Iso6luble    :Soluble, I Insoluble     Soluble   I Insoluble-   Soluble. Insoluble
                                                            &#xa3;           -           I                                      ____     I:                                     ____
Bromate                            as&#xfd;Br0
 'C 610 Chlorite                               as CIO' Cyclaexlamni4n..                   a's COH 13N-m 61. Diethylamine`              ------
p                                            as CIH,5 N0 01   63: Diethylamlnmoethanol.
  "U        Ethylamine*                        asC 2 HN n    64. Morphoyine&deg;                         as CAHNO 8.67                                    % by weight
      .66. Propylene Glycor                    % by weight
                                               % by weight Aerobic Plate Count m 70. Anaerobic Plate Count.
: 71. Fecal Coliform                     org'sti O0ml
     -72. IronBacteria.
: 73. Mold 0,                                           orgsm Nta!!Red..Reucers.                .org's/mi.
org'S/mi __
      -75. Slime Formers 0o        Sulfate Reducers I  18. Total_ Coliform                      og/ i .
0 Yeast, 9'
i Syste ImCapacity~
Proplionate'                  : as      2 H5O2                                                        --0.p00                    0.00 Total OrganicCarbon_                                                           1.&#xfd;67                       .95                  '.0......0<:6              1.40 total-Orgaic tatrogen--                              0..500                 ';0.500                     ,0.54 Mqexel(A42                                               2&#xfd;.
0                                              1.0                                              30
-      0   AUde. &#xfd;&#xfd;    H m-rb -  n91- . -   hWi&#xfd;mw

LABORATORY REPORT - WATER ANALYSIS                                                                          ICustomer No.:                       1001392 H.O-H Chemicals, inc. [ Rearding: Indiana and Michigah Power Company                                                                                                IReport.No.:.                 as indicatedj
                                    -500 S.Vermont St. ILocation:. Donald C. Cook Nuclear Plant                                                                                                    I.Report Date: .                                1 Palatine, IL.60067                                    1 Cook Place.                                                                                            Analysis Date:
Bridgman, MI                                          _Sample:Date:                                                                  as indicated 847/358-7400 Fk: 847/358-7082                       Control 11/16/06                      Treated 11/16/06                  ContrIol l122i06                 Treated 11/22/06                  Control 11/29/07
(#27142):                  I          (#27142)'                         (#27142)j                        (#27142)                          (#27177) ];
                                                                                        * .                                     .                                 ._                                    _      _I Soluble; I Insoluble                   Soluble I Insoluble               Soluble'        Insoluble,        Solublew.L Insoluble             Soluble I Insoluble 1.. Alkalinity ('P)'                           as CaCo 3                        o                                    0                                 0              0               - 0         ..           _           o il     ~
laiiy( a aO                                       3                  _                136                                                                12210                                22
      ,3. Alkalinity "OH)l        calcuIlted) as CaCOi, w        4. Free MWl Acidity_...                        as CawO3                                                                                                                                                                         .
a1 8. 5, ChemirCal   o,*i** e* - ;n *: .': .... .. .*10.95.....3.9.
Oxygen Dema (COO.)__
ChloroformExtractables                                                      &deg;.................... ...... ...----.......... .....---...................
                                                                                                                                                                                   ......... .... ...........---                4.00 ==* -
e       7. Dissolved Solids                                                          232                                 236                       ,       207                               218                               213 a.Hadnes (Calcium)                           asCaCO 3 ---.                  91                                   93                                82....                            83                               8.
: 9. Hardness (Magnesium)                        asCaCO 3                       49                                   49                                44                                44                               45 P 10. Hardness,(Total)                                 as1CaCO 1 -                   139                                 142                                126                              127                              ._130 r*1pH,7                                                                                                 81                  _           8.2                     _          7.9                                8.1 _
0      12. Specific Conductance                        pimhos                        M47            ....                 350                                315                              325                               320 p31.. SpecificGravit                                    mt..I e 14. SuspendedSolids .                         .......                                                 106                                                                510
                                                                                                                                                                                                                           .. 158133 30:0 r* :ui:* -i'u - .. -.....................
r15. Aluminum a-s-as At i-----------  ..... 0:01...   ...............-
1.42              0.01 i 0.21                  ..-000              0.45
: 8 .......50.191.. .---......----
                                                                                                                                                                                   ........ :0.00                          -... ;* . . ..5 0.01
                                                                                                                                                                                                                                              .o.............
6--
0.631
: 16. Barium                                      asBa                        .002               001               002              000               0.02            0.00             0.02             000              002           0.01 I 17. Calcium                                         asCa,                        36                5.81              37;3 .       .00                   32.7            3.66           '33*0              0.05             34:1          5;75 as 5 C-hnromium                                        as Cr                          0 OM_               000o              000              0.000            6000_            0.00             0:00             000             0.00           0.00 a1.Cp~per s I: o~ _*                         ' a~u:
asCu.            ......... qo........
0060              0.01..       .....
                                                                                                                        .0:00
                                                                                                                         ........... 000                   o.o 0.00             o.o 0.00             000 _00                          0.00I~o oo-o
                                                                                                                                                                                                                           .........         !oo 0&#xfd;02
: 20. Iron
                       -..    ............ ....a..................
                        -------_asFe,                                                000   ........... 22              6.6'0          -     43    i    ? 0 0  o
                                                                                                                                                              .00   .....i6  6 6 0.85,  .......   . 6 0.00o  i i .  ........
0431  , -  _-  ~0.00-;, -    -*
                                                                                                                                                                                                                                            &#xfd;1336 21Dea;_asP                                                   000            0&#xfd;000              0000            01 0              0.0              V0606             0000           0000              0.0600         02
: 22. Lith-ium                                  as LI                          0.00              0-00              0.00 -           0.00             0.00             0.00             0.00             000o            000:111- 9000
: 2. ageumas                             Mg                        11.8              2.36              119              005              10.7             1.29             10.8             013.             11;0         2.49
: 24. Manganese                   _             asMn                           000               009               0.00            '0.02             0.00             0.03             0:00             0.01             0.00          0.05 25: Nickel                                    as NI                          000               000               0,00             000              0.00             0.00             0.00             0.00            000            0:00
: 26. Potassium                                 as K                           1.48                                1.63                               1:.30                            149                               1 36
: 27. Siler           C 8 Sdu..aN.9.`a                          _              00                  000             0.001            0.0 0            0.00 6.40             0.00             0.00
                                                                                                                                                                                             &!62             000o             0.00         0006
:3*: *o *..........
: 29. Strontium
: 30. Zinc as Sr asZn 0.11 0.00 000
                                                                                                    &#xfd;0:03
                                                                                                                 . * . I ............... .........
011 0.01 000 0.01 0.1 0.00 0.00 0.00 .......
                                                                                                                                                                                            .** - : -:: * : ..................0.03 0`10 00 1 0.00 0.00
: 0. 11 0&#xfd;01 00.11 o' 31 Total.cation Millequivalents                                    ,              153                               1230                              2:831:                            2,865       __                   2.945 S2.Aetat                                     asOM          2                 0                                    0                               0:00   -                            0                                0 s       33. Bromide                                   as Br                         0*00 ,.                .             000                               0.00                                 00                            000
: 34. hloddae         .                      as,Ci                          12.7 c'*i rai*
: 35. Chlorate       ..........
                                  . ."- ........      as6C1      3 b
0.00                       ....... 133
                                                                                                                -----------1*
0.00              ~o              11.1 0.0      .            .           11.3 0.00                   ,          11.5 ooo
                                                                                                                                                                                                                             &#xfd;000 i
: 36. Chromate-                                  as C0 37.
_     luoide aP                    ___               0050,03,___                                                    _      000                               0.0004
: 38. Formate                                    as CHO 2                      000...000                            0790      .                                                         0.00                             00 0..
39 lclt                            s 2     3 0,               0.00                                 0.00                              000M                              000--                       -    000--
4.Molybdate                               &#xfd;asm   MoO                      000                                  00.__                  _           000                              0.00         ___                 0.00
: 41. Nitrate                                    as NO 3                        1:66                      _         1.38        _0940                                                   0.93                             108
: 42. Nitrite,                                   as NO2                        0.00                                 0000           _000                                                 0.00                             00
      '44. Oxalate                    __              as C20                        0.00                                 0.00                              0.00                              0.00                             0.00
: 45. P        (ortho)        .         a         04                    0.00               ___               0.00                              0.00                              0:00                _      _     0.00 A  .46-            sp ho-sphatN e (poy),-                     as&#xfd;(P0 4
    , 47. Phosphate (poly)                                 PO4            '
I1 .46.      Phosphoruis (total)                    ' as p                          000               0&#xfd;07               00.0                                0               0         _00               _00                  0.00          02 0      49. Sulfate         __             _           as S0 4                      '22.6        __                       228                               21.0                              24;4                 -           21.4 n 50. .Sulfur          . . . ... ..
Qtotl),                        aasS------7.93 s, 0 , N       ............
                                                                   .......                            0'00              &#xfd;809...............
0.00 _           7.16             000                39             0 00            823           ,0.00 51; Total Anion Mille~quivalents                                           3.602                                3.652                             3244                              328                              32 9-----
: 52. Ammonia&#xfd;                                  as NH3 ,
: 53. Benzotriazole                               as C6H 5
: 54. Boron_                                      asB                          0,40                                .026                               0.18                              012                             ,0.99           '
: 55. Silica,                                   assio .                        166M .            5.63             '1.56             1.08             .104              2.03             1.08       _1&#xfd;32                 1.16          '1181
: 56. Sodium Nitrite                            :as NaNO2 7.Sodium Sulfite         ____               asN 2 O 56 T.1yltiazole                                as CAHeN       3                                            _                               _________________
Medaa.        H1 aspwm"a sit                                                                                      Continued onreverse side.,

LABORATORY REPORT - WATER ANALYSIS                                               ICUstomernNo.:-                          1001392.
k-O-H Chemicals, Inc. Regarding:             Indiana and Michigan.Power Company                                          IReport No-.:                   as indicated 500 S. Vermont St. Location:              Donald C, Cook Nuclear Plant Dnad           . Cook     Nuclea   -oaton PlantI IReport Datew Repot        Date Palatine, IL 60067                      1 Cook Place.                                                               -Analysis          Date:
Bridgman, MI                                _Sample                                           Date:         as indicated 847/358-7400 Fax: 847/358-7082            Control 11/16/06           Treated 11/16/06             Control 11/22/06      Treaed 11/22/06                    Contriol' 11/29/07
(#27142).                   (#27142):                     (#27142),          (#27142)                            (#27177-)
Soluble   I Insoluble       Soluble   I Insoluble       Soluble. I Insoluble, Soluble   I Insoluble              Soluble.
* Insoluble F                           I             r                              ~--4                                                     -
: 59. Br-Omate.                      .-as.kp.   ..........
Chlorite.'   ...         ...
c                                      'as C10 2 Cytoexylamfne'*                 as C6 H 3 N m 62.
P 61, Diethylaminoethanol*            as C61 15NO 0
u                                     as C2 H7N4 Etrholanine*
n                                     a.C ywihNO 4
d      Diethylene Glycbl*              % by weight s
: 64. ProEylene Glyco.*
73;
: 70. Aerobic2Plate Count!            org's/ml M   75. Anaerobid Plate Count Fecal Coliform                  org's/ml C 77. Iron Bazceri*
r     Mold~
0 b      Nitrate Reducers 761 SlimeF ormers 7.gSulfate Re6ducers Total.Coliform a.
 .0      Yealst.
aorg.s o
_ org's/m.... -.
Residue.y Evaporation                                                                                   ............
o.
: 80. Volatile Solids a      Sy.stem Capac ity.
0.00                                                                                 0.00 Total Organic*Carbdn'                                                                                         __2.26                  21.6 Total Orgarnic Nitrogen                              <s0.500                        1'.,23                                                                               ojg mexel(A42                                                                            3.0                                               3.C
--   ii  PaOSSaxew 0Mm0~Thoermw0M&aamonlec.
                                                                                                                                                                                              'Li
                                                                                                                                                                                              ~s.

LABORATORYTREPORT - WATER ANALYSIS                                                                       Customer No.:.                             1061392 H-o-H Chemicals Inc. Regarding" Indiana and Michigan Power Company                                                                                      Report No,:&#xfd;                   as indicated 500 S. Vermont St vocation: Donald C. Cook Nuclear Plant                                                                                          Rbport Date:
Palatine, IL60067                             1 Cook Place,                                                                                     Analysis Date:
Bridaman. MI.                                                                                      Samole Date: as indicated 8471358-.740.0 Fak: 847/3.58&7082 Treated 11/29/06                              Control 12/7/061 Treated 12/7/06[ Control                                      12/14/06:              Treated 12/14/06
(#27177)                       .(#27204)                         (#27204).                         (#27246)                             (#27246)
Soluble    I insolubleI        Soluble I Insoluble,            Soluble I Insoluble                 Soluble!     Insoluble            'Soluble     I Insoluble
: 1. Alkalinity (P)                         asCaCO3 :                      4                                 0.                             0                                 0                      .       -    0 2.Akai~t      ~M)___asCCO-                          130    _          _               1214                            134                               124                                130
: 3. Alkalinity("OH'") j&#xfd;cIted) as CaCO 3
_4. Ffree Mineral Acidity                   :as CaCO..                     .......                                                                                                                                                   ....
a .5. Chemical O*xyLen Dernand(C.O.D.)                                      8.0                            1..1.8                          22.6
                                                                                                                                            &,__                                7.9                                14.7
: 6. Chloroform Extractables                                                                 ..... ..........
e 7. Dissolved Solids                                    "                 210                               230                             237
                                                                                                                                               ,203                                                                203
: 8. Hardness (Calcium)                    'as CaCO3-                    83                                89                              89                                79                          "       79
: 9. Hardness1(Magneslum)                   as CaCOq                    45                                 '47               ,            47                                '43         ..                       43
: 10. Har                                    asCaCO3                    128                               136                   .         136                              122                                 122 rl1.pH                                                          82                               779                              7                                 8.0                                 79 o 12. Specific Conductance                                                :t34hos 314           -337                                                350                              306                                 296 p 13. Specific Gravity                          I;L  l       ...
e .14. Suspended Solids_                        _60                                                                          315                           78.0                                  340                                 21.0
: 15. Aluminum                               asA]                      0.01            003              0.01             3,35           0;01             1.02              0.02               2.34             0.01               0.46
: 16. Barium                                 as Ba                     0.02             0.00            0.02           "0.03            0.02            0.01              002                 0.02            &#xfd;0.02               0.00 I17 Calcium                                 as Ca                     332              0.00            35:5            '31.3           35.6             5.75              31,5 -             25.9             31.4               1.87
: 18. Chromium
               .*er, c p                         as'Cr sC                   , 0o00
                                                                       .*o oo '             0:00*
o~           .6  00.
00         .. 0i O.........
O -..- ~ o.0oo 111-1.11.1-......--
0.00              0"00
                                                                                                                                                                . l.............                  0.01 6 .......... 0.00
                                                                                                                                                                                                          .0.00 :6"06                 *.6 a 9.Cope  -as                            Cu                    0.00             0.02            000              01.o                              .1000                              0.02        ~      .0               00 20l.r6nroas,                                    Fe                    0.00             0.05            0.00             6:03           0.00             1.56              0.00               4.34             0;00               0.54
       -. dLeadu ...........
: 21.                 - .. . .----. as'Pb                 .     ....-..--.
0.000           0.000         ..-
0.000    6  . 0.. .d             0.600 0000 *-   ......    **
0.00       ....     *
                                                                                                                                                                              .000     - " 0.004   *            '0.000 i oo            0.000o--
: 22. Lithium                                 as U                      0.00            .0.00            0.00             0.00           0.00            0.00               0.00               0.00             0.00              .0.00 23-. Mag R m                                                          1.8              0.0              11.5            11;0            11.5            1.98     __       10.5               9.3              1.5                071 6,
: 24. t/angan.se                ____.         asMn                      0.00             0.0(0.0000                       0.22           0.00             0.05              0.00               0.13            0.00                0.01
: 25. Nickel                                  as Ni                     0.00             0.00            0000                                                               0.00             '0.00              0:00               0.00
: 26. Potassium. ...            . . ...... as K              ... 1.53           .... ....         1.56 .       ..       ..-        1.65                   ,      - 1.23.2- . ..-                         1:25
: 27. Silver              ....                                          0.00            o                0.00             0.00       . 0.00               0. .              0.00         .0.00                  0.00               0.0o C 28. Sodium                                     as:Na                     7.02                             7.61                            7.73                               6.60                                6.65
. 29. Strontium                                  asSr             .        0.11             0.00            0.11             0.03           0.11 .           0..00             0.10               0.03             0.1n               000
: 30. Zinc                                    as Zn                     0.01             0.02            0.00             0.06           0;00             0.04              0.00               0.04             0.00               0.02 231.Total Cation Milleqt3iValents                                   2.896                          3095                                                                2.756 2.3100       _           _           2.758 32 Acetate                                 as C 2 H3O2                0.00                             0.00                            0.00                               0;00                                0.00 s 33. Bromide                                   as Br                     0.00                             0.00                            0.00                                O0.                                0.00
: 34. Ch.oiide .... . .. . ..                as:C                       11.7                             13.4                             14.1                              11.9                                11.7
: 35. Chlorate                                as C103                    0 00                                 00                          0.00                              0.00                                0.00 36 Chromate                                as Cr0 4                                   ,_--_----.......
:37. Fluoride                             as F                       0,05                             :0.00      _                     ,0.04                             0.04                                0.04 38      orae                      sCHO2                      0              0.00                                               0.0   __                         .000
: 39. Glycolate                              asC5 H3 O3                 000                              00.00                           0.00                               0.00                               0.00
: 40. Molybdate                     __        asMoO4                    0.00                           :000                              0.00                               0.00 .                             0.00
: 41. Nitrate                                as NO                      1,07     _2__03                                    .2.03.        2.13                               1.02       .           ...         ,1.10
    '42. Nitrite                                as NO 2 ___       _..      000                               o.o000                         0.00
                                                                                                                                           ,__                                 0.00                       -       0.00
: 43. Nitrogen    (totalJ)-_,                 N as------                       -------
: 44. Oxalate                              as C204                    0 00                           '0.00                             -000                               0.00                                0.00
: 45. Ph&#xfd;ospate (ortho)                       as          .             0....
00                              0.00                           0,                                 0.00 .00            .              0.0 A &#xfd; Pho                                      as P0 4
: 47. Phosphate (organo)                   as P0 4
: 48. Phosphorus (total)                     as:P                       0.00             0.14             1 01 01           0.00           0.45             0.00              0.00               0.15             0.00               0.03 0    49; Sulfate              .             _asSO       4                  215                              233                             284
: 28.                                22.3                               219&#xa3; n    561 Sulfur (total,                         as:S                       7.69       _    0.00           7&#xfd;97               119J           931              0,56       __7.02                    1.14    _       7.02                0.00
: 51. Total Anion Mill:quLvaLents                                      3.446              _      --   3685 1...                             31795                              3.342                                3,451.____
: 52. Ammonia                                as NH"
: 53. Benzolriazole,                         as CeHsNs                                           ........                         ....-
54.asB                                                                 05                             0.05                              001                                                   0.0
       -------  i -~c --                            S .O-.          . ...---..........--
                                                                                     ....       6 -,
                                                                                                  ,       2._  '          1 .0................. 6.---       .............
                                                                                                                                                   ----------3--9 . ...............
:3              ,,:'2.........           .........
5,Silica                                  sl    2                  10              0.26           2.25             110              2.Z26           3.9                1.37               8.42 .           1.35               Z.0 56& Sodium Nitrite,                         asNaNO 2 .
: 57. Sodium Sulfite                _         as'NaSO     &#xfd;3                         ... ..-....                                                         ......                .            .
: 58. Tolyltriazole,                          asCHeNj I
 -      . UdtoPI               rP=        ... hd.&#xfd;d                                                               Continued on reverse side.

LABORATORY REPORT - WATER ANALYSIS                                            ICustomer No.:                   1001392 HO0-H Chemicals, Inc., Regardindg Indiana and.Michigan Power Company                                                           IReport.No.:            as indicated 500S.. Vermroni St Location: Donald C. Cook Nuclear Plant                                                                IReport-Date:.
Palatine,.IL 60067                         1 Cook Place                                                              lAnalysis Date:
Bridqman, MI                                                                Sample Date:          as indicated 847/358-7400 Fax:i84/358-7082 Treated 11/29/06;                       Control 2/27/06       Treated 12/7/06       Control 12114/06                 Treated 12/14/06
(#271!77)'
(#27204)            (#27204)                 (#27246)                       (#27246).
                                                                            'Soluble    Insoluble    Soluble' I Insoluble 'Soluble    Insoluble  .Soluble           Insoluble     Soluble      Insoluble Bromate ..                 ....                  as:BrO 3__
60; Chlorite,                                          as dlO2 M            Cyclohexylamine*                                 as C6*0.
1 P                                                            as CH11 N 0
                                                             ,as C6H,5  6N0 I)    61.                                                    as C*2HN d                                                            as C4H9NO14
: 70. E&#xfd;thylene Glycol                                   % by weight e.
                                                               %by weight Prdpylene GlycW.                                                                                                                   -------------
: 74.     -erobic-Pla*te count.......                    org's/mi Anaerobic Plat Con C
     -- W"*
or/   OMmiL 67.
: 72. Iron. Bacteria                                   org's/0mi r    73.:
M7,                                                   org's/mi.
Mold 0
;b.
I:   75. Slime Formers Sulfate Reducers                                 o-..rg's/I0mi 0          TotaI'Coliforrn 70&#xfd; oa           Yeast7
.0           Residue by vaporation Volatile;Solids System!, capact                                                                                                                       _.. .. . .. .
Propiopnate                                      as C3H 5O2 -       0.00                      0.00                 . .0.                    5......
35*                         0.00 Tota Organic Carbon                                                  &#xfd;3.94                                                                                                 4439 Total'Organic.Nitrogen
                                                                                                                                                  '<081*2 4:5
                                                                                                  .      0............
                                                                                                      .... 14 0   A          flood0 in oais at 00n w              06000*0                                                                                                         W a.a    .a.I.  .S
                                                                                                                                                                                                    '5-)

LABORATORY REPORT- WATER ANALYSIS                                                                        ICustomer No.i                      1001392 H-O-H Chemicals, Inc. Regarding:                             Indiana and Michigan Power Company                                                                Report No.:               as indicated 500SS Vermont St. Location:                        DonaldC. Cook Nuclear Plant                                                                       Report Date:"
Palatine, IL 60067                               1 Cook Place .Analysis                                                                                         Date:-
BridgmanMI                                                   . . .                                Sample Date:, as indicated 8471358-7400 Fax: 847/358-7082              Control 12/21106                      Treated 12/21/06                Control 12/2806'                 Treated 12/28/06                 Control 1/4/07:
(#27294)                                (#27294).                      (#27323)y                      (#27323)                       (#27352)
                                                                                  *ll#nl*&#xb8;       I   IN*II0hI*                                          *,*hlhla       I In*nlHhla                                       0             1   .  -
                                                                                     -       -        -----        i -              - I             I'-.4**      .g                  I                          e I         u a4         s 11~F-Alkalinity ('P")                           as CaCO 3                         0                                                                    0                                                              0 A                               as CaCO 3_                                                                                          11.6]
: 3.         A..........I. as CaCO 3 -
2 Y&#xfd;             Free Mineral Acidity              _____    as&#xfd;CaCO 3 Ea              Chemial Oxygenemand (C.O.D)                             _                                                                                                                                                   180 10;                                                                                                      -........                                    6.4 t
Chloroform Extractables Dissolved Solids.                                                                                                                                                                                 .................
1'199 7
143 8., Hardness (Calcium)..                           aCo        3                  81 re                                                                                                                                                            79                              7S
___79                                                                                          80 Hardness Mgiesi m).                        as CaCO%                       .43                                     43                            44                             44 Hardness,r(Tot )..                         as CaC0O                                                                                                                                                           43
_124                                     123                            123
           .6. Specific Conductance                                                                                                                                                             123                            123
: r.                                                       pmh.s--           1.7                                  8.0                              8.20                             8.4; 8.0 0              Specifc.Gravity........ g/ml                                            308-Aq                                    23                                                          293
   'p; usended Solids.,
          --9.                                                                                              1.07                                3.0                                                           13.0                             305
: r. i20. Aluminum 16.
asAt                       ,0.02                                    0.02          0:03             0.02            0.80  ...............
9o       .......
:0.,_12           0.011
          .21. Barium                                        as:Ba                                                                                                                           .0.02 O23                                     0.02          0.00                                                          0..00            0.01           0.02
: 17. Calcium                                   *___as"Ca                                                 ............
                                                                                                                                 .o.....
                                                                                                                                .31.8      ___0:00             '31.6                           31.8          0.006            31.8           31.9
:e                                                                                                       0.00                                0.00            .0.02           .0.0-1
: 18. Chromium                                   as Cr-                      0:00                                                                   0.00                           0.00          0.00         .0.000             0.01
   $"                                                                                                                            0-.001        0.00                                                     _0 0
: 19. co-p-per                                   as Cu                      *0.O0              0  00 0..............
                                                                                                             ..                                                 0.00o          0.01            0.00          0.01             0.01           0.08 Iron                                       as Fe                                          :1.84.               0.00                                           .117                                          0.00
                                                                                         *0.00,            7.13                                0:05             0.00                           0.00          .0.18
: 21. Lead                                       :as Pb                    0.000                                   00.000 0000          .0.000          0.000             O.O          o.o00
                                                                                                                                                                                                                           &#xfd;0.000
                                                                                                                                                                                                                                 &#xfd;-o       0:000
: 22. Lithium'                                      ,~as'U                                                                             0.00                                                         0.000
                                                                                                                                                                                          -----0.00                          :10.6           1,.00 10.5                                    10.6                           10,6            1.45                          00.00
:24... Manganese            __                     asMn.
0.00             0.000                  0.00                           0.00                           0.00          0.00             1026            020 Nickel                                     as Ni                                                                             0.00 0.00                            0.00                   ..........
                          *F oit,m.----..........
Potassium.                   . ...............---
                                                       - -as K,  * ........  .       _.I.431 1....                       -.1-1-1........ ...-0.00
                                                                                                                                         .                                                                                   0_00
                                     ............. .....asg
                      .r.................                    a k -......                                   0.00                     31..
38
                                                                                                                          *...........                          1.43                           1.37                                  ..............
                                                                                                                                                                                                                                            .. 6 Silver                                                                                                ....
                                                                                                                                     -1%                      :0.00                   ........
                                                                                                                                                                                           ....0.00
                                                                                                                                                                                                 ,B 002                                                          0.00 C       23. Sodium*........ =i* ............... ...... .asias Naa*r_'
                                                                      .......                                                    8A12          0 00                                            6.65                           6:34 a                                                                                                          O:O                                                                                            0 00 It
                                                                                                                                '0.11          0.00          20. 10                            0.11                          0.10
: 25.       tnc*                                     as Zn"                      1.743                                   0.01                          &#xfd;0.01             00, 0--             0.0-0
: 36.                                                                                                                                                                                                                                002....
0             Total cation Millequivalents                                  ....-. 0.00.-                                 .2.762                         2.7.79                          2.,792                         2.769 n       37. Acetate ..                                     as C9H  3O                                                          0.00                         :0:00                            0.00 0.00 S       28. Bromide
: 33.                                                as Br                                                                                          ..0.00                             0.00
: 34. Chloride                                    . as Cl                                                                0:00                        .10.3                             10.5                           11.0
: 29.                                                                            0.00
* 36: Chlorate                                        as.C10i                                                                                           0.00                                                          0000 Chromatet                                   as CrO 4                                                           '0.00 430.
437.2. Fluoride
        ....                                                 asF                         0.01 0.00                                                                  0.0                             0.00                          0.00 Formate                                    as CHO 2 0.00    ........... .0_6o                          &#xfd;0.00
:0:00 0.00                .......
                                                                                                                                                                                                                        .....0.00
: 39.         yco-ata      --             -          as C 2HO  3 3                                                                                                                      0.00                          0.00 0&#xfd;.00
: 40. _Molybdate ..                                  aJs -MoOQ  4 0.100 Nitrate                                    as'NO3                                                              0.00                           0.85                            1.03                          0.98
: 42. Nitrite                                        as NO2                 _0.00                                         0.60                                                     .......
o..oo                         0.00
: 38.                                                                                                                     19.8o aN                                                               0100 OxalAte..                                                              0:00                                    6:59                                                                                         0.00 2O4 ....
                                                         -as.C 0i 004 Phosphate,(ortho)                          as PO0  4                                                        3.029*$                                                                                         0.00 0;00                                                          .0.00 A 47. P.hospat         P.._.(.orgaf sno)                  aSrP0 4 yOR
: 41.                                                                             1........
2 .                                3o0oo         0.02             0.04            0 04                          0.02                            O.S
 .0              Sulft             __                ------ as SO                                                                                                                              21 &#xfd;6                         21.4 n      50.
asas0S s.. ........            6:54
                                                                                                          '0.94                                                6.80            0.75            6.78 2.o90 0:62            7.!32 T~otal  Annion MLqiqlalents&#xfd;                                                                                                               3.142                                                         3,9?2
        ,52. Ammonia                                    as NH3 Benzotriazole                              as C6H N-Boron Silica as B as..S.......*-*. __1.44[_ 1.881*
0.001         I                .00                                                          0.82 1.12*]       0.00             0.00 0.00 1.08       _0    00                            2215                                                          9 78 "54. Sodium:Nitrite                             as NaNO2 SodiumSulfite                              as Na2 SON Toiyltriazole,                             as CH.N, A"              ARd.M exW      PHIn ;&#xfd;    pa ndlan a n indicaw                                                                       Continued on reverse side.

LABORATORY REPORT - WATER ANALYSIS                                                                                           Custbrher No..                          1001392 H-O-H.Ctiemicals, Inc. Regarding:: Indiana and Michigan Power Company                                                                                                        Report No.:*               as indicated 500 S.Vermont St. Location: Donald C. Cook Nuclear Plant                                                                                                                 ReportDate:
PFlatine, IL60067                                  I Cook Place                                                                                                     - Analysis Date:
Bridgman, MI                                                                                                         Sample Date:              asindicated 847/35847400 Fax: 847/358-7082 'Control.12/212/06
(#27294)
                                                                                                                         -Treated-12/21/06
(#27294)
                                                                                                                                                                -Control 121281/06
(#27323)
TTreated.12/28/06.                         Control 1/4/07
(#27323)!                         (#27352)
Soluble     j      Insoluble          Soluble        1&#xfd; Insoluble           Soluble              Irisoluble        Soluble      J      Insoluble,       SolubleI         Insoluble
: 59. Bromatel _                                       as BrO........
,o      61. Cycioheiylam ine'                                as;C,,H1sN m 62..D.ethylamine                               "          aSCHN.".........             ....                                    ",                                                                                                            .......
P      63: DiethylaminoethanolV                             as C6 Hi 5 NO
           -~Ethylarnine'
 " 65. Mbrpholne...
                                                     ----  -as Cztz    7 N.........                             .........                          . .,                                   ..........             .     .      ~
as C4HNO
:d :68. Disthiylene Glycol'                                 :%.by weight ___'_"
67...   :EthyleneGlycol                              %by Weiht                   ...                   ....            ..                         ....         .         .      ...
: 68. kroylene Glycol'Py                           %b.           ~ight                                                            ....           .                                                           .        .     . ....
: 69. Aero0bIc Plate count                     __org's/mt
: 72. Iron.B.cte..ia.                                                                                                                                                                                                          .
o 74i....r................
NItrat Rse          dubc.Paers ut             .....              ..                -.               -.-.........                                     ..............-
b      7 -. -- iare - e ,u .- -. - . . ... .org's/,mI                       . . . .       .........                                                                                                .              .     ....      .
i      75. Sline Foimers .org.s/l.l             ......
o      76. SulfateReducers.                                 org s/mi 77-                                                  oo---..   - -----                                                                           .     ........
                                                                                                                                                               .                  -g/.... .....         ........      ...     .        .....          ..
0   '81. Sulstem Capacityi b 80. Volatile Solids                              ......
ga       rl._ __     .....                  ..                     .       ...........                                                                                     ~     .            .
: 83. Total OrganicCarbon                                                             1.94                                     4.29                                3:09                                     7.7;8                            5:63 0 ..     . . .                ..... .     -.                          .   .             .       ....                               8.0....8.0......--
78.~~                 o+sm                                         *t 79.. R- esid u e.                 Ev      t.i n                          . ...              ..                       .       ..          .  . . .      . . .                .    .       .     . . . . .         . .     . by          .     .......
                 .e                     ..    .+.......                                                      .  ...... .. . . 0M         . .           .         .     .           . . . . . . . .. . . . ... ....

LABORATORY REPORT - WATER ANALYSIS                                                                 jCustomeir No.:;                           ibdi&#xfd;qz
                 '          *H-O-H Chemicals, Inc. Regarding: lndiana.and Michigan Power Company                                                                                             Report No.:                     as indicated 500 S. Vermont St. Locatior: Donald C. Cook Nuclear Plant                                                                                          Report Date:.
Palatine, IL 60067                              1 Cook Place                                                                                    AnalisisDate:
                                                                                            'Bridgman, MI.                                                                                   Sample Date:                    as ifidicated Fax: 847/358-7400 Fax: 847/358-7082                   Treated '1.4/07                      Control 1/11/07             Treated 1/111/07               Controal1(18/07                Treated'1(18/07
(#273.69),                 (#27436)                             :(#27436)
(#27352)                              (#27.369 q*h=hla        Ine^htkl*                                                                                                       -                1 m--
_   _      __    _     __    _     _        go,__ -e__     In      __        ,_ __   __a~-     __  __         &#xfd;jjj __        no   IngyIu                 _  _   _
Alkalini*/ty().                             .as caCO3                      120                                    0                             0                          12
: 21. Alk'alinity     (M'W)                        as CaCO3                                                  ...........
                                                                                                                          ..126
                                                                                                                             ._2                           126                        126                                 124 Free Mineral Acidity:                       as CaCOi:
a           Chemical Oxygen Demand (C.6.64                                .                                               17.9                          -18.9                         6.4
: 26. Chloroform Extractables a           .Dissolved S*oJlds...........................                             200                                                                215
: 27.                                                                                                                                                                            201
: 15. Hardness          (Calcium),                  n.CaCO i                      80                                    81                            :82                        '82                                    82 Hdnes(Magnesium)......... .s caco 3 44                                    43                            44                          441                                   44
: 30. Hardness CTotal)                                as:CaCO3                                                          124                            127                        126                           . 126 8.5..-
8:0                          8.0                            8.4
    ....22.
         -----                                                                         298                                  319                           323 133.Spcif~icGravit                                  g/m 14- Su~spendeSolids,                                                                               25.0                            195                         34.0                         38.0
: 25. Aluminum
: 15.                                                as Al                       0.00 S0;30                                          .......V;;                        0.01          0.59                  0.00              0,13 Barium                                       as'Ba                                           01;4                                                                                                           0.01 18;2             0.01 32.9                       32.7 Calcium .                                    as Ca,                                                           32.3          0.02                         5.42                         4.18                  32.7              1.76 18.
Chromium ......                              asCr                                           ,0 O03
                                                                                                        -0.01              0.00                           0.02 0.00        0.00                         0.00                                    0.00 Cppsr                                        as Cu                                                                         0.01                                                       0.00                  0.00              0.00 0.00 Iron                                         as Fe                                                            0.00         ,4.43                                                                            0:00              0.28 0.000                                             0.003               0.00 0.000        0.000                        -0.000                                  obooo 311. Lead                                         as Pb                                      '0.000              0.000                                        0.92         0:000                              0.000
: 20.                                                                            0.00                                                                            0.00 Lithium                                      as Li                                -         0-00              0.00                                                      0.00 M~agne._situm ...              .....         ~    ,g:-
10.6 0.000                0.75             10.6                           10.8                       10.7 0.00 0.90                                    0.00 0.03
: 21.                                                                                                                                                            0,.00                                              0:00 Mqanganese                                   as Mn                       0.00                0.02             0.00 o.o0 6.0c        0.03           o:oo 0.00          0.03                                    0.01
_22.: Nickel                                        as NI                       0.00                                 0.00                                                                    0.00                  0 00              0.003 0.00                        . O-le              0.00        0
                                                                                                                                                                     ;.00
: 23. Potassium                                       as K                        1,~27                                                                                          1.29                               .1.32 1.40                           1.65
: 24. Silver                                          a..
                                                        .Ag s                           0.0                                0.00                                                      0.00 0.00                                              .. ................;.~
Sodium                                       as Na                                                            6.70                                       0.00           6&#xfd;83                                6.89 Strontium                                    as Sr                                           0.00             0.10                           0.10                       0.10          0.01 C.                                                                                   0.00                                               0.27 W1, 419. Zinc                                         as znr                                          0.02             0.01                           0.01        0.18                         0.04 TotalCation Millequivalents                                            2.77,5                              2.810 Acetate.                                      as C2H30*                                                                                                                  0.00                                0.00 n0                                                                                   0;00                                 0.00                           0.00                                                          ;0;00 S           Bromide  ~~e Bro               ~~.................
m................
_           ..   .aBr. _ . ....                                                                                   0,00                        0 00 Chlbride,                                    es a...Cl                                                           12.7                           12:5                       11.3 25.~Chlorate                                        as C.                      *ooo                                 &#xfd;000 ChrbmateW                                    as Cr~
                                                                                 ..... 0:6                                                                                           0.00 oooo
: 48.                                                                                                                  0.00                           0.00                                                           0.00 0005                                 0.00                                                                                                            0:00 Formate                                      as CH0     2                                                     6'.00 0-----------                                                                                               ....
                                                                                                                                                                                                                                      . .._o.o....
0.00
:32i
: 27.          ..... te             ........       - as
                                                          - . C.                                                                                                                     omo                         -- 0;00, 52.
0.00                           6*
0.00 1.24                                                           1.29 Nitrate                                      as NO3                                                           17.51                                                     1.23 530.
            *i*tfte                               _.. as NO2 .                                                                                                                   1.00 Nitrogen (tqtal)                             as N                        0.00 0 00 Oxalate                                       as C 20 4                   0.00                                                                                           0.00                                0.00 as P0.4                                                                                         0.00
                                                                                                                   ---.       0 P~hospjhate (organo)                         as P0 4 0.00 0.00                                 0.00 Phosp!horus (tota)                           a                                              0,02                            0.0e                         0.00                                       ------  0.00 0          Sulfate               "                                                                                        23.3                         ,22.7                        21.3                                20.8 Sulfur,(total)                                asS                         o oo               0.54                            0.76             7.77        0.31           7.33          0:'87                 7.,38 Total Anion Millequivalents:                                                                                3.388:                        3.365                        3.325                              3.25,4 Ammonia.                                      as NH,.
Benzotriazole                                 as C6 H5N3
: 54.                                                                                                                  0...
700                                                    ,0.00 Boron                                         as B                                                                                            0.00
: 35.                                                                                                                  0 00                           0.00        o0 0 Silica                                        as       0iO 2                                    '0.83                          "7.75                                         0.00          0.00                  0.00              o oo S-o-di-um-    N-itirite      ..               as NaNO2 -
57.
Sodim Sl-ite- .                               as Na 2SO3 Tolvitriazole                                 as C H6N&#xfd;-    - .5. _______        _______            U              - L           ~                              .5.            4   -         &#xa3; _______
A.          Ni dalaOJCPI    P'1IApafis p&nt10     IA-stIIIAICSI0 Mau      &#xfd;PT P"Apaft       perft&#xfd; wniws&#xfd;                                                                           C.;ontnuea on reverse. sioe:

LABORATORYREPORT , WATER ANALYSIS                                                                                           Cust0mer No.::                                    1001392 H-O.H Chemicals, Inc. Regarding: Indiana and Michigan.Power Cornpany                                                                                                          Report Noi:                        as indicated 500 S. Vermont St. Location: Donald C. Cook Nuclear Plant.                                                                                                              Report Date:&#xfd; Palatinej, IL60067                                1 Cook Place                                                                                                       Analysis Date:-
Bridaman. MI                                                                                                        Sample Date:.: as indicated 847/358-7400 Fax: 847/358-7082                Treated 1/4/07                               Cntriol 1/11/07                         Treated 1/11/07                       Control 1/18107                  Treated 1/18/07
(#27352)                                       (#27369                                (#27369)                           (#27436)                              (#27436).
Soluble:          Insoluble                  Soluble 1 Inso                          Soluble.
Soble           Inblule              soluble    I Insoluble            Soluble         I     Insoluble
: 59. Bromate:                           as BrO 3 q      61. Cyclohexylamine*'                     CsH1 m ias 6     3Ne~
                                                    . ,D   _ y~ -- ..............
m...ne*..-a,.s.C~iH:nN. ..        ..     .         ....        . . ...            .  ...    . .     ....       ..   .--                                                          ...........
P, b. Diethylamrnoethano                      as C4 HNP D                                    c...... .3.
_64.. &#xfd;Elhylamine___                    as C HN n 65. M Trpholine*                            as CHNO                                                                                                                    ..           .   ...                                                     . ...
d .66. Diethylene;Gyc01_                      %:by weight
: 67. Prhylene' Glyco....               '%.by weight 6...         9. ............
                                      .     ._% w lh             .....   ...........                                     _                                                                                            .
: 69. Aerobic Plate count               rorgsm                                                  -.                     .   -.                 .          -              ...-.                  .       ..      ,         .          ...
M 70. Anaerobic.Plate Count                   Org's/mi iI .71; Fecal Coliform                        org's0100ml                                                          .
C72. Iron Bacteria r .73. Mold           ___                     ogsm S          isi Reduers__                    org....s.............       .............                    ....      ..... .. ...............           ...         ...   .                           .   ..        ... .                                   -       -......
0 7c 8 Slime     Farmersoor:ss/mi e s .0                            gsm                                                                                                   .......                                                     .     . ... . ..           .. ..               ....
   '0  76. Sulfate Reducers.        ___       org s/mI.
       ?7. Total colaiorm Cb                  org's 1                    1:ml2....2.....
: 76. Yeast                                rsmi                .....                   ...                                                                                                                                           .              ..
_,79: Re i u-----------                                                                                                                                                                         ---
I~.....                                                                              .       -- ----  - -__
                                                                                                                              --.                            ~.                                               .           .             .          .        .
: 81. S tem c         y.         .                .        .      .      ... ...                          -------........                                                                                           .........
      '82~.Propionate.            _           as C H5 O                   .00                                               0                                     0                                0.00                                  0.00
: 83. Total Orgianic carbon        __      _1                        0.20                                           8.22                                   9.07 64., Total Organ~ic N~itrge                                        .. 6                                       0 0                                    <0.500 85.~                                                                                                                                               .0.
                                                                                                                                                                                              ..........                                    4.0
                                  .i... ...          ...-                                                 -- -- -                                                                           - - -.......
                                                                              .~~Ll                                ..... ...
h             d                 ...       .....                                                                                                                                                  m ae

LABORATORY REPORT - WATER ANALYSIS                                                                                                  Customer No.::                                         i00i392 H-O-H Chemicals, Inc.                             Regarding" Indiana and MichiganPoWer Company                                                                                                    *Report No;:                             as indicated 500 S.Vermont St                           Location:            Donald C, Cook Nuclear-Plant                                                                                                 Report Date:
Palatine, IL60067                                              1 Cook Place                                                                                                               - Analysis Date:
Bridqman, MI                                                                                                                 Sample Date: as indicated 847/358-7400 Fax: 847/358-7082                              Control 1125/07                             Treated 1/25/07                          Control 2/1/07                            Treated 211/07                             Control 2/8107
(#27429),                                 (#27429):                                (#27466).                     [           (#27466)                                    (#27502)
                                                                                    , Soluble r          Insoluble                  Soluble       I Insoluble              Soluble         I insoluble               Soluble       J    Insoluble              Soluble            I     Insoluble
        .1. Alkalinityk("P)                              as CaCO3 :                                  0                                          0                                       8                                        6                                          10
: Alkalinity-("M')
                .2.                                      as CaCOj,                               120                                       .120                                    122                                      120                                          126 3.Alkatnlty ("OH')(c.lo.......s.C.C.
: 4. Free MineralAcidity______                            CaCO..                               .s                                                                                           .......
a        5. Chemical Oxgen Denmand.(C.O.D.) ....                                                 3.9                                        .7.1               __                 11.8                                        7.0                                          7.4
           .6; Chloroform Extractables                                                                        -      -          ...- -------
: 7. issolved Solids.2221 a                                                                                                                                                                          2112029 r       a.'Hardness (Calcium)                            as CaCO3                                  82                                        82                                       81                                       80                                           B0
:9:        'ds      M_es              ,.                      CO3                          45                                        '45                     .                43                                       44                                           45 P     -10. Hardness Toial)                               as:CaCO 3                               127                                        127'                             _124              ..                            125                                         125           .
ri          p                                                                   8.2                      _                   8U_                                     8.2                  ___                 8.2                                       8.1.
      --' piSP Spei
: 12. p     ,*:: ..*~ .,._
codcace.h317                      I**
t..;_     * '_ ...........
                                                                                     .- I . .. ..... ..... ___              ::::..**....318    :.......___             :::::310: :                        ::::::: 30.: ::: : :: :: :::::310 : .....
p 13. SpecfcGaiy                                         gm e 14. Suspended:Sol1ds                           -                    ...
* 427                                    37.0                                      10;0                          ....__ 110                                    .      .       234 r 15. Aluminum-                                          asAt                                  0.01           . 209                      001                :050                  001                   019                 001                      0.13              0,01                      19g8.
1-1 ....:
L&#xfd; 16. Barium    *0 .. 1-as Ba  -7    ..........   .......-...
                                                                                        -11   .111 0.02-   -1...........001                 --
0.01-- :    -.-.....00-67 O_       1........ 6;002 ... . 0"I        00:6o11  ....... 1_02      ..- ...11o1- 60.00 -6..... *a002* -o                       0,01 o
1 17: Calcium                                          aCa:
a                                     328q                  12                   38                  1.06                32                    0.00                322                      0.00              31.9                  -   135 e18. Chromium:                                       as Cr                                 0.00                  0:00                 0.00                0.00                0.00                  0.00                  00 0__                      00        00              o                 0 d01 a Cope  ~             ............asu00              19.                                                         0                   0                01                  00                    00                    .0                     0.0               00                        0.00 20 ro         _                         sFe                                 000                  357                 0.00                 075                 0.00                  0.24                0.0                      0.0               0.0
_qg                       3.38
: 21. Lead                                           as Pb                               0,000                0:000                 0 000                06000                0 000                  0.000              0.000                  0.000              0.000                      0.000 22 Litium                                          as Li                                 0.00                  000                  0;00                0;00                000                   0 00              .0             :00 0.00                 .00                            0.00
: 23. Magnesium                                      asMg                                                        584 5109                 108                 057                  10.5                 0.54                10.7                     0.35               11.0                     4.56
      .24. Manganese                                   .-asMn                                   0.00                 0:11                 000                 0,02                000                   000                 0.00                     0.00              0.00                      0.09
: 25. Nickel                                         as Ni                                 0.00                  0000                                                                                                  0.00                      0.00                   00                   0.00
     ':26. Potassium                                     as K                                   146                                       163                                     1.29                                      1.34                                        1.06
: 27. Silver                                      .0                                                                                 0:00                 0.0                    .0                 00000                                        0.0
                                                                                                                                                                                                                                                    ,00                                  __      0._..00
              . ..e ........                                                ..                  0:00..                      0             0~00.0.o...0.00.....                                                                                          ..............                           0.
C *28. Sodium                                            as Na                                 7.37                                      :7.70                                    6.74                                      6.74                                       6.44
: 29. Strontium:                                     as Sr                                :0.10                 0.02                 0100                 000               -011                    0.                  0.11                     0.0,              0.11                      0.01 Zinc                                       as Zn                                 0.00 030        ._0.02                         0.00                 0:01                0.01                  0.02                0.00                     0.05              0.00                      0.05 S31 o,290         Total Cation Millequivalents                                                 2.896                                                                               22.8 2.811f                                    2.817 17                                   22.908
                                                                                                                                                                                                                                                                    *807 n.3-2. Acetate                                           as C2Hd02                             0.0                                       000 .0.00                                                                          0.00                             -         00..........
000 Pro33.                  -                  as-Cl.0.00
                                                      "'mde     Br  'g;<;;*]*~~~~~
                                                                          "--                  goooo 2.000                                   6..                                      o-oi
                                                                                                                                                                                  .6 ~ ........ .......~                    ooo ~ "oo ...........              ......          .
34.Chloride                                as cI                                   22____                                   10___                                    10.2                                       0.                                       9.34
: 35. Chlorate                                       as CIO 3                  -           0.00                                      0100        __                             .00                                     0.00                                       0.00
: 36. Chromate;                                      as Cr0 4
    --3-7. Fluorid                                       asF00                                            _             __               00                                         .0000                                                                              0.00
: 38. Formate                                        as.CHO2                               0000                                                                               0.00                                      0.00                                       0.00
: 39. Glycolatea                                  _ as C2HO 3:.....                        0.10                                      0                                       .___00 0.00                                      0.00                                       0.00
          .        ybdate     '                          a MoO4                                0.00                 '                    000                                      0:00                    .                 0.00                                       0.01
: 41. Nitrate                                    as NON                                1.13                                      1.08         .                           0.00                                      0.00                                       1.05
: 42. NitrIte           .as                             NO2                                                                                                               0.00.0.00.00
: 43. Nitrogen (total),                          as N
    .44. Oxalate                                     as.CzO4                                                                                                                  0.00                                                                                 000 ;0.00 4'&#xfd;5.Ph&#xfd;ospha&#xfd;te (ortho)                           a P04            -    -   -     -    -    -   -    -     -       -   -    - -   -    -     -    -     -                  Pl                 __                      000                                           00 .......
                                               ....       ...........                                                                                                                                                              .        ...                      ..........     . . . ...   . .: t -
n 47; Phsht(ran)                                       aP0 146. Phosphorus (total)-                         asP                                                                                                                        0.0 .00
                                                                                                                                                                                     .0o-----------.o0-    00               000 00                       000 0         __ 000                            oT o
o04. Sulfate                                    as So 4                                                                                                              _22.0                                         22.1                                       224 o u lf te
: a. .... O         .       .. ....... .. . ..         .     ...                                                                                                       .                             .......2......... - 7, n 56. Sulfur (tl......as                                        S         -         -                                                -__                                          7.68                  0.75                7.60                     0.52              755                           50 s 51. Total Anion Mlllequivalents                                                   #VALUE!                .                      .
                                                                                                                                 #VALUE-           .                           3                 -206                     3  165 "3.                                3267                            p. .
   -..          -      .    .                            _ ...                                                              --        4               --      .----.....-.-...--
5      Benzotrlazole                               as C6H sN3                                                                                                                                         -
: 54. Boron                            ,           as B                                                                                                                       0.92                                      0."63                                      o_0:00                      ..
55 Sliaas                                  S10                                                                                                                00                      0                 0.00                     0.00              0.00                         000 8.Sodium Nitrite            _          _as         NO 2            -#VALUEI                           __             #VALUEI l-                                                  ____
: 57. Sodium Sulfite                                   s    Na 2 SO3          ...........                                                              .........                    ..............
: 58. Tolyltriazole              .                 as      CiHBN,                            .          .           .           ...                                                                                              ...............    . ............           ..............
ADdab.pHi        padsaWrdoonoas*ds*i           oled                                                                               Continued on reverse side.

LABORATORY REPORT.-,WATER ANALYSIS                                                                                dCustomer No.:                       i001392 500S. vermont St             ocaton-: Donald C.. Cook Nuclear Plant                                                                                   Rep0rt-Date&#xfd; Palatine, IL 60067                       1 Cook Place                                  ..                          .              ....               Analysis Date:
Bridgmah, MI                                                        "                  "                  I Sample Date:              as,indicated 847/358-7400 Fa-x- 847/358-7082           Control 1125107              Treated 1/25107                                  Control 211/07                Treated 2(1(07                  Control 21807
(#27429)                         (#27429)                                     (#27466)                     (#27466)                          (#27502)
Soluble:       InsolubleI    Solb~ble          IInsoluble                 &#xfd;Solubl6      "Insoluble    I   :Soluble          Insoluble     Soluble     IInsoluble
    -11.
59,1- Bromate
                     ...           . .          .. aas B  r 3 Br0 3 C _,60.. , Chlorite
             ,--,..r...
                     ._....      . ...... ...... *s      C 9 ....
                                                     .as.Cjz 06.Cyclohdxylamin&*                            as C6H13 N m    62. Diethylamfne                                as C4H11N
: 63. iet ylarpinoethano_                   ,asC  CH    15N0 64j. Ethylmn                                    a'sC2 n65. Mopholjne                                    as C4H9 NO d' 66. Diethylene.Glycol-                            %b. :eigh
  ' 617._  Ethyleine Iyc1                            %,b weigh ea.*Prolpylene.Gly~col:'           .,           % byweighl
: 69. Aerobic PlateCount                          orgsm M 70. Anaerabic Plate Count.                         org's/mi 1 71.' Fecal Colitorm                  _             org's/lo00mI c    72. Iron Bacteria 73 Mold
 .b 74. Nitrate. Reducers                            orgs/mI 5SieForrmeirs                            ogs/i_                 .  .   ......          ................                 . ..                             ,                                 ........ ..
: 07. M Sulfate Reducers                               orgs/mi.
: 77. TotalColiform                     n-            s 7.8,. Yoa east  1                                 org s/mn                 .. .          .. ..   ........                   .       ..                 .          * * *...             .... .. ....... . .          ....-          .......
I M Residue by Evaporation                                 --
i-- .....            --   ........ .* .....    .....
80e.Volatile Solids:
a 81. SytemnCapacity                                 gal 6,3._ TotalOrganicCarbon                                                      *     ~~ ~ ~               ... ...................
: 64. TotalOrganicq Nitrogen 65 Mexel (A-432)                      "
                                                                         .....                        . ... . .................. ..                                                   ....          :Z..............
   .. 0..... at: .0z0--o-Iin0.4".'
S?nEIa....... kat odi* iii

LABORATORY REPORT - WATER ANALYSIS                                                                  Customer No.:                     1001392 H-O-HChemicals, Inc.                          R6garding:-Indiana and Michigan Power Company                                                                 Report No.:.           as indicated 500 S. Vermont St. Location:                           Donald C. Cook Nuclear Plant-                                                                 Report Date:
Palatine, IL 60067                                   i Cook Place                                                                                  Analysis Date:
Bridgman, MI                                                                                  Sample Date:           as indicated 847/358-7400 Fax: 8471358-7082                        Treated 2/8/07                 Control 2/i5/07                     Treated 2i/5/07            Control 3/1/071              Treated 3/1/07
(#27502)                            (#27541)                        (#27541)                  (#2763"1) '                  (#27631)
RtInhhI. I  In*.nl*,        R'fh 'hl           I  In. hhi,      QAnl.h     I Innh.h*        nhlhl. 1In  fhhi,  I   AnIhl.       I n1nhhdl I                ,1                                      ____---___                               ___                       1 0
1:
                       -Alkai iniM).........
Alkalinity ("M")
as:CaC* 3*:
asCaCO3 126 144 6
144 0
a
: 4. Free Mineral Acidity                           as CaCO3           ,...............
                                                                                         .                  ........                                         "7.7                                                  6.1 w      5. Chemical OxyenDema'nd,(CO .D.):                                            16:6                                                                                            5.4 B. Chloroform Extractabies e     7. Dissolved        qSolids                                               ' 210                                                                                                                          208 232                              235 r         Hardness.(Calcium)                            as CaCO3                                                                                                                      87                         86 579             ............... 9                                 95 Hardness (Magnesium)                            as caCo 3 .                                                     .49 50                        48                          46
'P     10. Hardnes'_(Totat),                                as.CaCO3                   1125                                     5..........
                                                                                                                                  .                         145                       133                          132
: r. 11..             -S---------------      .................
                                                             *.. . . . . 8.3                                                                      8.1                                                     8.2 0        Specific Conductance    .              .            m!__                    312                         ........
348*                             350                                               _-304 P
13!.                                                                                                                                                                      _.0 .
Susp&#xfd;ended Solids                  ___                                                    4.5                                     7.0                         7.0                      83.0 3116 Aluminum                                         asAl                      0.01          0.08                 0.00               0.09      I   0.01        .O.OE                      0.83                               :0.03 "t                                                                                                                                                                                  0.01
: 15. Barium                                            as Ba                     0.02 U00 0.02                             0.02         0.01                      0.00.
4.75 0.02                 0.00
  ,a        Calcium                                        as-Ca                       31.8                                                  0.00          38.1         0.00                                      34.4 124,Chromium                 _                        as Cr                                  0:00                                                   0.00         0.01         0.00          :0.06          0.00 a8 0.00               0.00 copp..            .                              as Cu                     0.00          0.00 0.000                                                  0.00         0.0c         0.00                         0.00                 0.00
:20. Iron                                                                        0.00          0..10 as Fe                                                          0.00              0.000                       0214        0.000          1.48 21:. L.:ead:                                                                                                     0.000                                                      o0000                        0,0oo 0.00
: 18.                                              "_ as Pb                     0.000                                0U00                             0.00       0.00C                      0.003
      ,22.                                                                                                                                  0.000                                                                                      0.03 Lithium              _                        4 SU                                             ,.....................                                                                 0.00                                 0:00
                                                                                                                                                                                                                          ........... 0o......
00
: 18. Maesr.                                            as Mg                                   0.00                  12.0              0.17                       0.0c.
0:0C                      -1.5                                V000 0.00)                                                                                                                      0.00
: 19. Magnesu            ..         ....         .......as-n.                                  0.000                                    0.00                       0.00         0.00         0.06            11.31               0.00 0.00 25.
Nickell                                        as Ni                                                          0.00               0.00                                    0.00         0.00              -0.0 0:00         0.00                                                                0.0c Pcitassium                                     as K,                                                      __1.53 1.42 27.; Silver                                           as Ag                                                                           0.00                                    0.0c                         0 00 c    28; Sodiurn                                           as Na                     8.45                                                                                                                       8.49                 0 00
                         "                                                             0.11.0                             7:51                             0.10 6.56 a    24. Strontium 29:                                                 as.Sr                                    0.00                  0 10               0.00                       0.0(                                      0.11_I_..
I t"                                                                                                                                                      0.00                                                                        0 00
                                                                                      '0:00         0.03 I.        Zinc                                           asZhn                                                          0.00               0.02                       0.02                      0.02            0.01 0.00                                                              3,261,
:0.00 To~tal aio Milq                     et's                                  2o.oo                           3,.255.                                                                                  2*963 n
si AcetatFe                                    -s-   ----
2                     0.00                                                                                          0.00 Bromide                                        as Br          -                                                                                                                                      0.00 0.00                               0:9.0                                                                                   0.00 Chlordide           ______                     as Cl                      '0.00                                13.5                                                       11.4                        1t1.3 Chlorate                                       as ClO,                                                                                        &#xfd;0.00 392.                                                                                                                                                                          0.00                         0.00 0:00                                                               3.2.6o Chromate                                       aS Cr0 4                                                      0_100                             0.11                                                   0.10 Filuoride"                                     as..s F                                                        00                                                         0.10 30; Fo-7 ......
                    -t -   -...... ........
                                         . ..                    C . ......
0:00                                                   0.00 Formate                                        as CH0 2                                                                                        0.00 0.00                      0 00 0.00 Glycolata __            -.           .         as C2H.30                                                                                                                                      ............
k-9,9 4:4Z 33,  Mybdate                ..                    -a~sMoo 4                                                        0.00                                                       0.01 0:98                               1.84 Nitrate                                        as N.O3                                                                                                                   1.45                         1.29 Nitrite
: 50. Nitrogten(oal.)
as NO2                                                         0.01                                                                                    0;00
                                     ..       .            as N 0.00                                                                                          0.00 oxalate -                                      as c-04 _                                                      0.00                                                                                    0.00 0.00                               0.49                             0.00                      -0.00 Phosphat.(ortho)               _               as-PO4                                                                                                                                                -0.00 A              Ph .pat&#xfd;(qr@oiy) _                         asPO4.'                                                                                                                   0.12 Phosphate [organo)                             asPO,
: 38.                                                                                                                             ... Q.oO           0:00 Phosphorus (total)                             a Ps                                     0.oo                  0.00                                          0.01                      0.05            0.00
 .0         Sulfate..                                      as SO 4                                          ...           24*4                             0.81 0.......
00:6                              _21._2 n         Sulfu01910)
(tota).               .......                      .as.S                       0 00              .. 80.3_8                                                      7.44                                              0.00 Total -AnionMieuvert                                                     13316                                                                                            290
: 47. Ammonia         ,                               asNH3&#xfd;,
53 Benzotriazole                                  as CaHoNs Boron'                                         as B:                                                         *0.1                              0.10         0.60         0.6c 55.
Silica.                  -                     as S0 2                     0 00&#xfd; 0-::*..0.9       ... oo          0.00                                   2.38 1:99 0.06 000 Sodium Nitrite                                 as NaNO2 sodiuL     Sulfite                      . as Na_2SO T0lVitrazole                                   as C4H.Ni 6          Anl dt eept pH InPob W mpliu a, as                   atdked                                                            Continued on reverseside.

LABORATORY REPORT - WATER ANALYSIS-                                                                                                           Customer No.:                                     100139',
H-0H Chemicals, Inc.                               FRegarding: Indiana.and Michigan Power Company                                                                                                           'Report No::                       as indicated 500 S. Vermont St                          Location: Donald C. Cook Nuclear Plant                                                                                                                    Repo-rt Date:
Palatine, IL 60067                                         1 Cook Place                                                                                                                            Anatysis Date:
BHdgman, Ml                                                                     ......                                                  Sample Date:                      as indicated 847/358-7400
                                                  'Fax: 8471358-7082                             Treated 2/8/07                           Control 211 5107                             Treaied 2/15107                                Control3/l/07                            Treitid W1i/07/
(#275602)-                 j              (#27541)                                   (#27541)                                   " (#27631')                                  1(#27631)
Soluble     [    Insoluble.               Soluble'              Insoluble         .. Soluble,          Insoluble                   Soluble:               Insoluble           Soluble          I   Insoluble,
       .         ornate              .                                as Br0 3                                                                                                       ..................
51    cyclohexlamine*                                          as CgH, 3N____-
a                                        --                                                       ------rnne---    .......
0,':3 R ietylamninoethanol ......-....................I..........I
_      as, 6H15 NO                                                   w............. ...........----.......--.............--...........
                                                                                                                                                                                                                 ...........                         i.........- ............--.......                      __
n     6.9&#xfd;Morphoiin&                                                 as c4 H5N------
S-        E he*-------                                                      w i..                -.
                                                                                                     ----- .............                    .       ...... . ..       ..... I ...........
65    P tyleneGlycol                               -           % by weight
       ...                      Plate. ur                               rg m..............nt wmi - .........                                .---------- ------             -- - -                                .   .      .        --                                                  .. ....          -
M 70. iAnierobicr Plate coun .. .rg's/miL                                                                                                                     .                I ...      .. I                            I                                       ...                     . . ..                    .
Feoacoiiform I _............o
      -t                                                               rg'. ._   Ionm-----------...........
c 72. lron'Bacterla _
: 73. Miold                                                      ogsm
: 74. Nitrate Reducers                                           org's/m                                                           ...                  "              ...                                               .
*o  I75. -o,"Silme Yeast, Formers                                           org'smi                         _                              -       -        ..      .-----          -     -        --.---.-.                     -          -               -rg-                      .,------m'....-........
O7.Sulfate              Reducers                 _         _org's/mi _
771                 co.lr                                            /l m                                                                                        .ota. ....
9 _&#xfd;                                                                r.smi__                                                               ....  ... ...
9esdu byEvaporation c   '0. Volatile Solids
: e. a.........        C pa it .                                                                     i* -222 Z i                                                                                                       _-                                            -----------.....
82.. Propidnal:6                                               as              2                 .000....
0-O                                        000                   0~0                                                                 0.00                                     o.0              .
683. Total Organic Carbon
: 65. Mexet_(A*-42)                                                 "2.5                                                                                                                  1.0 .                       ...                                                 .        2.5
              ." .- . : . .I .....
                             .. ..... . .           +... . .. .. .. . .. ........ . -----
                                    ....       .                                             "+"..... .-.----
                            . __ .                                                                              _. . .. ..
* _ +    :.+ : .     .     . . ..                        . ...    .      .. .             . . ..     . . ..                      .. . .     * - .       .......      ........      ....
                            + -- ---
                       ------.              -* -         . ...    ..       .. ...    .... . . . .. .... .                                                              ......                                            - i -          = .. *      :                                                        --
                                                                        .. ....                        . . :"~~  ~~ ~~~ :*-.+.                                     ..
0    AU!!W.                nN In  fl. -      AMn ., .. indi-iml                                                                                                                                                                               A.01,4. bv

LABORATORY REPORT - WATER* ANALYSIS                                                                            Customer No.::                   1001392 H-O.H Chemicals, Inc, RegardingL IndianaBand Michigan Power Company                                                                                            tRep0t No.:               as-indicated 500 S. Vermont St. Location: Donald C. Cook Nuclear.Plaht'                                                                                                  Report Date:
Palatine, IL60067                            1 Cook Place                                                                                               Analysis Date:
Bridgman, MI                                                                                               Sample Date:             as indicated 847/358-7400 Fax: 847/358-7082                   Control 3/8/07
                                                                                 *(#27631)
[treated     (#27631) 3/8/07            Control 3/15/07
(#27677)
Treated 3/15/07
(#27677)
Control 3/2/07
(#27694)
I.,~IrhI~             *,nti hl*    I   Incnh =hlo,      *nh Ihfm     .l In*P, hlhl=i      *knhlhl     I,   In nh,.hl,     ,&#xfd;-t~hf.       InnldhihI 4:                             ___                 ___...~.                                                                           ___            ____
Alkalinity ("P")                              as CaCO3                  0                                   0                                10                                                               10 Akai nity('l'.) ....                      . as. caCO 3.
: 3. Alkallnity:("OH") (ca~cum=I)d               .as caco 3 ;
132                     ..... . 134                             ....:126
                                                                                                                                            ........      2                                126                           138
                                                                                                                                                                              ......... ....12 Free Mineral Acidity                          asCCO                                                                                       20.7 a    .5.
: 9.          ChmclOygen Demand (C.OD:)_                                                                        4.4                                                                                               14:6 Chloroform .Extractables e   17: Dissolved So lids                                                                                                                                                               2200 209 99                                 99                                                                    79;
_4. Hardnes(Cal)               ....... 'as.Caco       as CaCCI                                                                                                                          43                            46 a           (Mgneium
: 10.         p*. : .....       ...............                                                                                                     124                                                              133
                                                                                                                                            ,82 "_"16
    .311-                                                                         2372                                                                                                                          8.2 Spcfionductance                          prnhos o
: 14. Suspendedra ..S            .......... ....-...-.. .........                            20 0                                 ,54 0                           __543                              19.0                            .280 3959                                                                  30:8 15: Alumiinum                                        asAl                                                                                        0.01                                                             0.01 0.02                                              ,0 03              0102           0.2 16;. Barium                                          as Ba                 0.02            :0.00              .0.01              0.00            0:02                                              0.006          0.02            0.02 Calcium                                       as Ca                 39.4            o.00                39,5             1.47             32.0            42.7                                             34.6            25.3 Chromium                ___                   as Cr       -                                                                                                                  0:00 0 02 0.02                0.00             0.oo             0.00             0:00                                            0.00 0.00
                                                    .. ....      Cu
                                                           ....s.a               0.00                                                  0.03            0.00            .0.01.                                           0.00 0.3                 0:.00             0.78                                               0.00 0.000 10.0             9.31                                                          0.005
     .21. Lead.                                                                                                   0.000             0.000                                                               0.003
                                                                                                                                                                                                         -0.00,
                                .as                              Pb                           0.000                                                    0.00         0.012                0.000
_22.. Lithium                                         as Li                .0.00            0.00                                                  0.00             0.00                                                           .0.00 13:. Magnesium-
:23.
12.6            0.00                12:6              0.97                             17.0              10.6           0.08             -- .
1.12           9.27 0.00 Manganese                                     as Mn                 ~0;00
                                                                                 &#xfd;0.00           0:01                 o&#xfd;c              0.03
                                                                                                                                                 -" 0.00 0.33              0:00 0.00
:0.7           0:00 0.00 0.24
     ;25. Nickel                                         :as Ni                  0 00            0.00                0 00                                               0.00                                                            0.01 Potassium                       .___ as K                                                                                 0..00...o__.1.34
: 28.                                                                       --:4
                                                                             ---------                                                                                                    1.30 o:oo            0.00 sodium                                        as Na                                                                                       0.00             0.00              0.00                                          0.00 0..00.....66                                       6......
                                                                                                                                                                                       -.-657 G 28.                                                                           8.44                                8.52                              6;61                                              0.00 strontium                    . ....---------  as--- Sr ...                                                                                                 0.04 O6                                                               M.03 0.00                                                                                                                                   '0.600 Zinc                                           as Zn                 0.12                                0.01                        ........
0.1o&#xa3; 0.oo0                              0.00           0.01 0.000 n
0         ,Total Cation MILLequivalents&#xfd;                                       0.0
:3.424                             2.792                        -2.7*67
  &         Acetate                                        as CAHO     2 34;7                                                                                                     .0.00
: 18. Bromide                                          nas   Br 31.
: 19. Chloride,                                                                                                                                                                                                      0.00
                                                          -as    Cl
_                                                                            151                                                                  11.6 53: ---
* or-'. 7 ..............     .......
     ;32. Chlorate                                      as -igC0 ......       0.00                                0.00                                                                :0 00 Chromate                                     as Cr0      4                                               0.00                              0,00 0.11 34.oo  Fluoride                 _                    as. F                 0.00                                0.11                                                                                               '0.11
: 20.                                                                                                                                              0.00                               011 0.00                                                                                                22.61 36.
0.00                                                                                                                                   0.510 Molybdate,                                     as MoO4, 0 00 0.00                                                                   1.27                                                            0,00 2.24                                2.32
: 40. Nitraten,            al.                       as NO1                                                                             ...............
qo                                                               0.01 Nitrite                 -as                NO
: 42. Oxalate                                                                                                   0_00                                                                  0.00
                                 .               .         as CiQ4                                                                                     0.00                               0.00 0_00 43: PhosphateW(poly),                   .            as P0 4 A                                                                                                                                                                                        0.00                          0.00 nTi 450.
Phosphoru~s (totl                           :as P                               ...........                       ... 0.00                 21.3             0.03                               .00 0-----         *1.94           0:12
: 46. Sulfate;                                         asSO4                 24:2                                24.6 n                                                                                                                                                    7.01 47.
Sulfur(total)                                  as S                                                                        0.00                             1.02              6.79           0:00 a   548.Total Anion Millequivalents                                                                                                                                                  3.*356 0.00 3 740                             3.364 Ammonia "..                ..                as NH 3 "
Benzdtriazble,.                                as C6H5N3 0.0c 5.szBoron                                          as B                                                       0.00                                                                  0.00
                                                                                                                                                      '0.00
: 52.                                                                                                            2.40                                           22.43                  1.32                                       11:"77 Silica                                         as S11   32 2.41                                                  1.60            1.34                                               2.16 Sodium Nitrite                               as NaNO 2 Sodium Sulfite                               as.Na 2SO3 TolvItriazole                                as C HbN 7    1 A-W        -lld." Ipl     4 pH~
l Ip        rno-~            ~aO-                                                              Continued on reverse;side.
iy~Y

LABORATORYREPORT - WATER ANALYSIS:                                                 ICustomer No.:                         1o01392 HO-H Chemicals, Inc. Reaardirim Indiana and, Michiaan PoWer Cdrmanv                                                            I Reoort No.:,               'as indicated 500.S. vermont St. tLocation: Donald C. Cook Nuclear Plant IReoort t
Date:
Paiatine, IL60067                       1 :Cook Place lAnalvsis Date:
Bridaman.MI                                                                       ISamoleDate:                as indicated 8471358-7400 Fax, 847M358-7082               Control3/8/07          Treated 3/8/07               Control 3115/07          Treated 3/15107' "                   ontrol,3122/07
(#27631)                (#27631)                     (#27677)                 (#27677)                           (#27694),
                                                                  *Soluble    Insoluble   RSolubfleh I   1ln lbl       Soluble      InolubhlA    Aoluble-       Insoluble         Soluble    I Insolublel
_____ -      --   -   -- - -    -    -.   ...           -    -     -     -    -le                     I--
: 59. Bromate                              as BrO..
chlrdite                            as CIO 2 vc.ohexy*lamine*                  as CjH1.N m       62. Diethylamine*                        as C4H11N p      63. Diethylamihoethano_                  as C         NO 0                                                         1 iH U                     n -tyam
                            ---------- _ -              C2H7N&#xfd; n           Morpholine*                         as CHsNO Pi   :66. pofietylien~lcl                     %by weight
: 67.    !elthylene               -
                                  -------             bMtyweIght Phopy!teneG!yco .          i         byweigh.t
       '69. Aerobic Plate Counti               &#xfd;org's/mi o           Anaerobic Plate Count              &#xfd;og's/mi                                                                                      . ..............
70:
       ,71. Fecal Colifoimf                              logsio~ml Iron Bactera'
              -ok --.---------                          s--i
_73.
:iI.75. Nitrate'Redu slime Formersogsm cars          or~gs~ml 9      76:. sulfate Reducerst                   org'sri
: 77. Total Coliform                        o rgs/1      MI
: 78. Yeast                                org'simI
.c     79. Residue b         vprtio I           Volatile Solids System rCapacity          ._        gal._____
Propionate ....               3 asoC
                                          ......                       0~i0                                                  .o a
                                                                                                                         ........                                                       0:00   _______
63:
Total Organic Nitogen                 ...
                                                                                                  'oo
     .85. Meel (A-432__..            .                 .                                   .... 1.0o
                                                                                                                                                                           - I.,. ____________
_________________       I IiiI7IiYi7T 0   A] d&#xfd;       .4      -             Ind    t

LABORATORY REPORT - WATER ANALYSIS                                                                       ICustomer No.::                          1001392:
H-O-H Chemicals, Inc.             Regarding:      lndianaand Michigan Power Company                                                                   Rep0rtNo:                as irdicated 500 S. Vermont St. ILocation:, Donald CI Cook Nuclear Plant                                                                                   Report Date:
Palatine, lL 60067                       I Cook Place                                                                                        Analysis Date:
Bridgman, MI,                                                                                       Sample Date::             as indicated 847/358-7400 Fax: 847/358-7082           Treated 3/2207              ,Control W328/07'                   Treated 3/28/07                       Control 4/10/071             treated 4/10/07'
(#27694)                    (#27725)                                  (#27725)                        (#27790),                    (#27790)
Soluble; I Insoluble        Soluble     I Insoluble            ,Soluble           I  Insoluble       Soluble    I Insoluble       Soluble      I     Insoluble.
________________                                    ____         -              K             -            -      ____                 - ____          z                            I ____         -    ____
Alkalinity ("p')                   _iasCaCO 3                *10                             8                                                                    -- 0 as CaCO_3                                      ......126s                                                                     132                          13X as CaCO Free Mineral Acidity              Sas c~a~cO 3 ...
Chemical Oxygen Demand                                        8.2                       16.2                                       7.6                             6:6                          7.5 t   16.
Chloroform Extractables e          Dissoved Solds'--                                           222                         209                        .... .............
312..
0209                             211                         '222 r
                                                .a~s CaCo 2                                             82                              .........82 45                              48
   -20. Hardness(Magnesium)                                               46                          45                                                                      436 15..
132                                                                                                   136                  -.---...1837 oH                                                                                                                                                       8.1                          8:0
                                                               &sect;.2-312                                                                                                   &#xfd;323 13.
nded Sotids
_Suspe&#xfd;e 21.;5                            154                                     1.0                          2.5 1.2i7 Aluminum                           'as All                 '0:01           0.26                  ........    --- ?                0.01            0.04            0.01         0.06            0.01                   0:06 Bariurr                               sB                   .0.02           0.00         0.02               0.01                                   0.00            0.02         0.00            00                     0.00
                                                                                                                                            *32.9           0:00             35.4         :0.00 L.7.1   Calcium                              as Ca                  34.4           0.00        "32.8               12.2                                                                                35:8                   0.00 Chromium                             as Cr                                                                 0:00                                                                0:00 o.0c 0.00                                                                                                   o.oc 0.00 0.00                                                                                 0.00 0.00
: 29. LOWpe                               as Cu                 .0.00                                                                                 0.01.            0.00 Iron                                 as Fe                 0.00            0.4E         0.00               3.14                  0.000            0.20 ......... _0*..q 0.08                   O:OC                   0.06 0.0c       O,000C                                 0.000                                                                                  0.o00o
: 11. Lead                                ,as Pb                0.000                                         0:002                                   0.000            .0.00        0:000         0.00c
   ,21;
: 12. Lithium ..               .          .as U                   0.00                                                                           .........
0.00                         0.00            0.0c                   0.00 24:     Magnesium                            as Mg                  "11.1 A.l0 o..o__.
0.00C 0.0c        :1,1.0 0----.-..
0-               4.05                   11.0           0.00             11.5         0.00            11:.                   0.00 0.00 0.00 ............
s                                                                                                                          .
: 14. Mang~ans Nickel as Ni                  0.00                        0.00               0.15 0.00
                                                                                                                                                                            .0.00         ~~
                                                                                                                                                                                        . 0..00...        0.00                   0.00
: 15.                                                                                                               0.00                  1.29            0.00           .0.00                          0.01
: 26.                                                                                                                                                                                                   .1.45 Potassium                            as K;                  1.35       __O.OC 0.0c         1.31                                                                      1.56 Silver                               as Ag                                                                                                                                                                            0.00 0.00 8.
0.00                        0-.00,             0.00               :0.00              0.00 .... .. 0 .0.            0.00            80.0 C          Sodium                               as Na                  6.57                        7.09                                     6.95                             0.04 0&#xfd;00 a         Strontium                            asSr                                  OOC          0:11               0.01                  o:11             0.00            0.12                       1140
                                                                                                                                                                                           .a00 0__
130Zinc                                     as Zn                  0.01           0.03         0.00               0.05                  0.01             0.01          :0.01                          0.01 0        Total Cation MillequLvalents-_ -                                                                                             2.884                             3.153                            13.3.4 Acetate                         ___as.C2!73O2 .              0.00                                                                 0.00                           '0.00 12.65                                                                                                                              0.00 8         Bromide                              asBr                   0.00                        0,00                                     0.00                             0.00                         O.Oc 12.6                                                                                                                               13:7 Chloride                              as Cl                                              116                                       12.1                            13.4
: 36. Chlor ata                               asCrO                   0.00                       0.00                                      0.00                             0.00                         0.00
                                                                                                                                                                                                         .0.00 37.
Chromate                      sCr0 4 0.10
                                                                                                                                                                                                                   ............. b.....
Fluoride!                            as F                                                                                                                         0.11                         0.11 3a.                                                                                                                                      0100
* 0.00 Formate                              as CHO 2               0.00                        0,00 0.00                       0.00                                                                       0.00                                                bOlo
: 28. glycolate                                asI 3C H 3 0                                                                                   0.00
:0.00                          0.00 Mo/ybdate....                         as MoO 4                                           0.01                                     0:00                                          0.08            0.00 30*:
1,.
402; Nitrate.                                                       1'.79                                                                                                                              1.32 as.NO 3                                             1.22                                     .1.13
: 52.                                                                                                                                      o~oo                           .0.00 Nlitit Nitrogen (total )
                              ~                 a No as N 2 0.00                         0.08                                                                                                   0.00 534 24.
xalate '        ........          as C20 4.__                                                                                  0.00                                                        .0.00 0.00                        0.00                                                                       0.00                        0.00 0.00                                     O.00 A  27. Phosphate (poly)                        as P0 4 Phosphate_(organo)                   as P0 4      "
358.                                        as P                                   0.0c                                                                   0.01            0.01                        0.02
          ,Phospphrus (ta) 0 0         Sulfate                              as SO4                 22.4                         21.0               0.60                  21:.8                            22.2                        22!7 n         Sulfur (total)_&#xfd;,.             "     asS                                                6.93                                      6.97                                                                                 0 00
.S                                                                                                                                                           0.00                                      3.561
         .Total Anion Millequivalents.                              3.632                       31357                                  3.384                             3.546 Ammon a                               as-NHA3 Ben:zotriazole                       as-C6 HN,3,                                                                                                                                                                       0:10 Boron                                asB                    0.00                        0.00                                      0.00                           :0.00                          0.0c 1.i37                                                                                                  1.05 Silica                                asi0                                                                                                                          1:05 1.64           2;01                           .5:67                                  0.49                          Oo~o Sodium Nitrite                     as NaNO 2 .
Sodium Sulfite                        as Na 2 SO3 Tolyltriazole                         as C?H 6 N3 ARdate MeeppH p   P~14Pe nin n urse 1*u*te5d                                                  ,Continued on reverse side.

LABORATORY REPORT - WATERANALYSIS                                                                                  ICustomer No.:                              !1001392 H.O-H'Chemicals, Inc.                 Regarding: Indiana and Michigan Power Company                                                                                          Report No::                asiridicated 500 S. Vermont St.            Location: Donald C. Cook NuclearPlant                                                                                                  ReportDate:
Palatine, IL60067                                      .1 Cook Place                                                                                               Analysis Date:
Bridgman, MI                                                                                            Sample Date:               :as indicated 847/358-7400 Fax:1847/358-7082                  Treated 3/22/07                                Control 3/28/07               Treated 3/28/07                      Control 4/10/07              Treated 4/10/07
(#27694)                         j          (#27725)                     '(#27725)                          (#27790)                           (#27790)
Soluble                ' Insoluble            Soluble j      Insoluble       Soluble          Insoluble          Soluble       LInsoluble;     Solublew                  Insoluble
: 56. Bro m ate . .
C, 60. Chlorite
                                   . .a o,.+,+,+c_o_,_..... . .
_as s .Br03.......................................
C102
      ~ ~
a                  xlmh
_. ____"*      '       ...     ... I~co*
H;
* 61.               _. Cyolo*....aC...
                                                                                                                                                               -aC
                                                                                                                                                                 ,        -,, ""--~i21;* ,*,,,*
                                                                                                                                                                                              - -"- ---- --- 1-* * -           l2111:i ......              11..;..l.
i p63RDiethylamlfotao                            asCH        5N0
   ---      D     ylen e         y                            w.....                                    ...   .......                                 .                                                                     .............................. .
6_3 5
5.... o pp Ol-                               as                      .....                                      .............. _
I -------   -
d    66. Dlelhylene Glycol'                           % by w~eight                  .___
69: Aerobic Plate Count-                         org's/m.                    .                                                 .          .        .                "......        .........                                                         "-.-.-...
M670Anerobic Plate Count                           org's/myi                                                                            ____            _________         ..
Mb 70. NitrateRedcers                                 org's/ml 0   75 Slire-Formers                     ...     . o rgs/m .                     .                                                                                                                                     ......
-b        1,*21** T;* K _... ....... .o-",- - i----
: 74. Sulfate Reducers                             org s/mL                     --- - .. ... ... .- .. . -. ... ?                                               ... .. .............           -- ...
1   78. Yeast_           _   __                      org's/ml                           .-                                                     -
o      O Volatile Solids i "- sy.tem
_,81         Capacity                   _ gal.                                                                ............
:83. TtOrgahiic Carbon                             o                                                                                          ./.                              .           ...
4... Tota Orai  -              t g
                                 -------.....  ....-.    ...            .-                                  .    -     .      ..      . I ..--
8      M2     .     .     .    ...                                                     ..      .                            .       .                                                           ........   .                             .      ... ....
                     ~
             -~~~~~~~~~     5C1 nonnn~no.               ninn         0nd2                                                                                                                                          Aavusb           heaei~

LABORATORY REPORT - WATER ANALYSIS.                                                                                 Customer No:!                      1001392 H.O-H Chemicals, Inc. Regarding: Indiana and Michigan Power Company                                                                                                        Report No.:               as-indicated 500 S.Vermont St. Location: Donald C. Cook Nuclear Plant ...                                                                                                       Report DE Palatine, IL60067                                  1 Cook Place                                                                                                  Analsi [E.
Bridgman, MI                                                                                                   Sample Date:              as indicated 847/358-7400 Fax: 847/358-7082                      Control 4/19/07                Treated 4/19/07                           Control 5/3/07                     Treated 5/3/07                   Control 5/10/07
(#27817)                            (#27817)                              (#27972)                           (
(#27972)                         (#27972)'
Rnlfble   IF ln-hnhl.          RM,.hlh            I 1n-1hrhl.         *nlMhf.       ] I-Iulthln            qnlIhlhl     I  In nuhlhl,'           nhh   I   in=,nhJhlR Alkalinity ("P")                             as CaCo
                                                                             -r-,         0
                                                                                                  -         ____                   -r        -    1~~n 10
                                                                                                                                                                                        ~               .. ____
4.1 3                                                                                                                                                       ---   10,
                                                                                                                                                                                                                                 -- 138 162
: 3.  .Alkal.nity('COH.)..i!
P~y(~')               _             as CaCO Ca-C-- 3_* -                                                  1:0 FRee Mineral Acidity                         as .CaC0 3                                                                             .......... 11    ,
a       5: Chemical OxygenDemand_(COD )                                             15.4                                                                       :61.                               65                               6.5 t      6' Chloroform Extractables B       7.
Dissolved Solids 263                                 263                             =-218                                   227                              229 1,                                                                                                                                                                                                93 Hardness.(C            .iu.m).....-.. as-CaCO_                          118                                                                         88                                                                :92 Hardness (Megneskim)                         5 aO                        62                                                                  ,47                                                                       51 103 Hardness (Total).                              as CaCO                                                        1092                                  1...
                                                                                                                                                           .... !34                                 144                             143 r,                                                                          8.0:                                                                                                        8-2                              8.3 803" Specific Conductance                                                                                        392                                                                                                 .....343 0                                                         tmhs ..                    399                                                          8:2'            7                              341 p
13: specific:Gravity.......9mi
_14,
               &sect;Supended Solids                                                                       175                                      8.0                                 1.(                                "1.0 0.02                                                                                                                       .1.0 r            Aluminum                                     as Al                     0.02           1.7C                                   0.15                0.01                 2.
00.....          0.01               0.01          0.01             0.01
 .t1           Barium                                       as Ba                                                         0.03 0.03           0.01                                   0.00                0.02                              0.02                             0.02             0:00
: 17. Calcium           ....             .         asCa                      47;3                                43.5
                                                                                                       .8 6.85                                                       35.0             0.0s              37:3              0.00          36.8             0.00 S                                                                                                                                        .0.0,0
      -18      Chrbdmium                                    as cr                     0.01                                0.00                                  0 00             0.00             0.00                             0.00            '0.00 19..           Copper-         ~                   as Cu 0.00                                                                      0.00                                                               0.00             0.00
       .20. Iron.                                        asFe                                     3.68                0.00               2.83                                                  0,00               0.02                           6.02 0.o00                                                                     '0.000                            0.000          ,     0.00 Lead
      -21., Lithium.                                        as.Pb                    0.000           O.O0              0.000              0.000 9-C o0......0.....               ...............
                                                                                                                                                                                                                   - -oo 0.000         0.000            0.00
        .22.                           ___                  as LI 0.00                '0.00              0.00                0.00                                                               0200             0.00 Magnesium                                              _asMg
                                                                                                     -2.51                13.9 15,0                                                                                       0..0
:0.00                                              12.3 Manganese
__        __            .......as       as.Mng    _                                                                                                          0.00             0;00 0.00           0.15                 0.00                                  0.00                              :00C                             0.00
:25. Nickel                                         as NI                     0.00           0.00 0.00
                                                                                                                                                                                                                                                   &#xfd;0:00
        .26.
Potassium.                                   asK:                --------
6*                                  1.58              0.00                1.35             0.010                               0.00---- 1.28 0110 Silver                  -                    as--
Ag--------                                                                                                                                                          0.00
        .23.
C             Sodium                                       as Na                                                        668                                                     0.00            . 1..........3.                     723 Strontium_:                                  as Sr                                                        0.11                                   0.00                              0.00 297.                                                                         0:13
                                 .as           .Zinc           Zn        .            ,001                               0.010 0.00 0.22 0.01         ......o..oe         0.11 0.05 Total Cation Millequivalents .                                       4.045                             3.735                                  3.019                             '3 232 0                                                                                                                                                                                                                              3.197 0.00 n                                                                            ---     000                                0.00                                   0.00                              o~o0 Bro0riiide  ...... ..................... :ass Br.
Chlorat                                  C.....
O
: 31. Chloride
: 38.                                                 asCI                      15.7                                                                      11.9 Chlorate                                    :as C103     _             0.00                                                                      0.00                                                               0.00 0.00                                                                     0.0E Chromate                                     as Cr0 4 .
Fluoride                                     as F                      0.10                              ,0.10 0.0:00
                                                                                                                                                               '0.09
:0.04                              0.04                             0.00 Forinate.                                   .as CHO 2                                                     0.00                                                                     0.00 39.
G-lycolate           -                       asc 2H03                  o6o0                                                                      0.00 40.
                                             --                                       0,05                              o002                                                                                                       0.00 Molybdate        .                            as MoO 4                                                                                            0.02                              0.01 0.00                              ,2252                                                                      1.68 Nitrate                                      as NO -                                                                                             1.40                                                               0.32 Nitrite                                      asNO.                                                                                               1.06                                0.4                           0.00
                                                                                                           .... ........ 0.00  . .
Nitrogen (total)        _          _         as N
      -40. Oxalate                                                                                                                                                                                                                 0:00 as C204                   0.00                                                         ...............
O* o                              0.00
: 35.                  (orio)---
Phosp...a.e                              as P0 4
                                                                                                                 ....... o~oo
                                                                                                                        ~0.00                                  *000 A 38.
Phos.hate(poy)                               as P0 4                   0.00                                                                                                        0.00      . .........
n      41 Phosphate (organo)                         .,~
0 00                                   0 00                                                               0.00 I. 542 ,Ph6sphorus,(total)..                      .        s P                                     0.0                                    0.00                                 0.00                                0.00                           0 00 Sulfate,                                      as SO 4   _                                                  25.1                                  21.8                               23:4 n
-0            Sulfur (total)                                asS                       8_60                                                                     6.99 0.00                .804              0.00                                0,00                                  0.00                           0.00
              .Total Anion MitequvalenI                                                                              4 397                                   3. 624                            3 888 57.1 Ammonia                                       as NH3                                          ........... .H.. . ....
457.           *, -- - - -__~      ..------
                                                .. --.--       .sC
_ .... H.N Bnzbtriizolem                              as C6H&#xfd;5N3                                                                                                                                                                            --
0.09
: 50. Boron                                          as B                       5,84                               3.24                                                                     0.08                             0:02 1.68 Silica,                                       assi02 ,                 2.26            6.12               :200               0.82                3:04              0.00             3.18              0.00 Sodliu Nitrite                               as NaNO 2-.
Sodium Sulfite.                              as Na 2 SO%
Tolyltriazole1                               as CHAN     3
      -       AndWB*&#xfd;     phI   ~'P.nnhn~,
bnen                                                                                           Continued on reverse side.

LABORATORY REPORT - WATER ANALYSIS                                                                       lCustomerNo.:                                    1oo0139 H-.O-H Chemlicals, Inc. Ftegarding:                                 Indiana and Michigan Power Company                                                                 Report No;:                  .      as indicated 500 S. Vermont'St Location: Donald-C. Cook Nuclear Plant.                                                                                                    Report Date:-                                  1/0/00 PalatineIL 60067                      -                  1 Cook-Place                                                                                       Analysis Date:                                 110/00 Bridtman, MI                                                                                       Sample Date'-                       as indicated 847/358-7400 Fax: 847/358-7082                         Control 4/19/07                 Treated 4/19/07'                Control 5/3/07                  Treated 5/3/07                              Control 5/10/07
(#27817).                     '(#27817)                       '(#27972)'                         (#27972)-                                    (#27972).
Soluble, I Insoluble, I Soluble.            Insol6ble I       Soluble'. Insoluble I &#xfd;Soluble                    Insoluble             Soluble              Insoluble-
: 59. Bromate                                            as Oo.Chtorite.                                           as 0102 0    61. Cyclohexylamin*                                    as C6 H13 N                                                                         ..........          .......
    ,,6.2.Oieth~ylamin&                                       s 4H 1            ---------
63."*Dlethylaminoethanoi*                               as C5 H15N0               ..........                                                     ...
6,4.Ethy~mn                                             as C21-1    7              ...........           ,
: n. 65. Mr.pholine                .                          as.CHNNO                                                                                         -------------   -----  ----  ............       ........
d 66. Diethyleie Glydol*                                   'W~yeight                                                     ......       ....
fT
: 6. EthyleneG-yco-l-*-----                                   byweight                                                                                                                                        .........
: 69. Aerobic Plate, Count                                 rsm
  *M70 " nerobic&#xfd;Plate Count!                               org'simi I 71. FecalColiform.f . ...................
                                        .                                  Irg's(Om,                                                                                                                                .....
: 73. Mold                        '                     org's/mI 8 74.          itrate Reducers                              org's/ml I 75& SlimeFormers                                         org's/ml o   78. SulfateReducers                                     _rgesT
: 77. TotalCotiform                                       org'si100m1 0   78. Yeast                                               org's/mt                                                                      ...
9 Residueby Evaporation                                      _...        ,                                             ..........
c 60. VolatilibSolids                         -
a 81 System Capacity                                        gal
: 82. Propionate,                                         as-C3 H502
* 0.66,                          0:00                            0. 0                               b.00                                      &#xfd;0:00
:3.Total Orgahic Carbon                                                                                                                                                                             ---------------
   ,.84. Total!Organic Nitrogen .....
85.---                         .exl.....~                                                               1.5                                                                   2,5
    - _, .... .e n     -.. n.a ...................
                 ... . .. .  ... ....     . ... . ......... -=. . ------
AnalvMs bv Gas Chr*maWamvhv.%

LABORATORY REPORT - WATER ANALYSIS                                                                                  lCustomer No.:.                              1o06392
(~~                        H-O-H Chemical, Ind. Regarding: Indiana and Michigan Power Company                                                                                                         Report No.:                        as indicated 500 S.Vermont St. Loaauon: Donald C. Cook Nuclear Plant                                                                                                              Report Date:
Palatine, IL60067                                    1 Cook Place                                                                                                   Analysis Date:
                                                                                               .Bridgman; MI                                                                                                   Sample Date:                       as indicated 847/3.5877400 Fax: 847/358-7082                      Treated 5/10/07                    Control 5/17/07                            Treated 5/17/07                    Control'5/24/07                      Treated 5/24/07
(#27972)                          (#27958)                       j           (#27958)                           (#27999)                              -(#27999)
Soluble I InsolubleI              Soluble I Insoluble                         Soluble'        Insoluble          Soluble,      Insoluble             Soluble        I lnsoluble I
: 1. Alkaliity,("P-)                                          3                     10                                  0                                         0     .as.CCO .                   0 -                                    0
: 2. Alkallnlty;("M"                              as CaCO 3                        134                              152                                        154                                122                                    124 3.s Alkali                         as-a--------...........-               -----------
                                                                                                        -------        ~    .    .-        .                ...         ..-..
: 4. Free Mineral Acidity                             Cas CaCO3                                                                                                           _
a5. chemfIcal Oxygen Demand (c.oo.)                                                    7.1                             16.0                                       16.3                                 5;9                                    6;3 t . C hl o ro fo rm E xtra cta ble s                        - ----  -                                                                                                                               .....                                                       ........
        .7. Dlssolved'Solids                                                             216                              231                                         236                                2.14                                   210 r          H d.cHardness.(cal.i.m.                                                        8~1                                               .as.               .. 8.. . ....                            68~.....                              68               .........
: 9. Haasess  ... . .. Mg*PG
                               ..* p. . . . .               ..   ... .. ... 2                                                4k6a.i47.40     ........        --------- ...........             7.8                                                40 P %10. Ha~rdness &#xfd;(Total) .....                          as cacO33,                       136                               M___13                                      3                                 108           __                      108          _      _
r            Clp                                                             8.2                               7                                1        :,        38                                               .78.0.7  1.       ...                          O
: 12. Spec0fic                                                                      327346                        0onductanceos                                                                                                                   -46031.-30.00"-
P3. Specific Gra~vity.                         -g.                                              ___                                                                                                                        02 415.Aluminumg'nes                               asA[                    _        00 U1
                                                                                    -----------          -0.004           0:01.0 ... 14 0                                          00                 0.15            0.01        . 00.08 . ...              000              0.02 16 aNium                                          asNBa                          0.02              .0.00           002                    0.01                0                   000    _       002              0:00                  0.02             0.00 17w. Calcium                                    asca                           35.1 0               0.00           342                    152                 348o                380            V.23             1.440                 273              0000 e      18. Chromium                                      ascr                           0001               0.00           0.00                   0.01               .0.00               0.01            0.03             0.02                  0.03                 9001 0.19.cop                                        as cu                          0.00 ..            0.00      _ .0.00                  .0.00                  0.0.0          .0.0       -         :               0.00                .000                03 0 iron T2.                                        as Fe.                         0.00               0.10           0.01n                  2.76                0.01               0.32             0
                                                                                                                                                                                                        .20.00            .202                                   0.02 21.20 Lead o a su                        ...... ass K Pb "". .                   1.2     .............
                                                                                         .0.006009                     0.000 ..........................
                                                                                                                                                 .           . -. 0.00 AR3 .4             0.0 0.00                        0 00p 0
* 0.00 -----------   0;000-1,--
: 2. Lithimi .                                      as LI"               -         0.00                     0,00 0.00                       0.00                0.00                                       000                            0.00
: 23. Magnes~ium                                     sMg_         -_               11:8               0.00          '11.                   .4.08                11.5               0.76             9.69            0.17                  9.63 _. 0.00
: 24. Mhanganes                                     aslMn                          0.00               0.00           0.00                   0.1.                0.00               0.01             0.00            0.01                  0.00              0.00 25.&#xfd;Nickel                                       asiNI -----                     0.00 0.0           600
                                                                                                            .     -    -i0.06                    00                  0.00     _          0.00           '0.00            0.00                  0.00              0.00
: 26. Potassium                                     as-K                           1.22                              1.38                                       1.43                                2.07                                  1.90 217.silver                                        as'A                           0.00.              0.00           000                    001---
O           _       0.00                0.00            0.00            0.00                  0.00              0.00 c 28" Soima                                      a86                                                        79                _.2,                                              .          8.47                                  83
.a     29. Strontium                                     as Sr                          01                                0.1 00                    02                  011                0.01            0.12             0.00                  0.13              0!00
: 30. Zinc                                          as               -             0.00               005         "600                      0.17                000                013             001              045                   0.00              030
: 31. TotalcaonMillequlvatents,                                                 3 4                    __      _2993                _3052.583.__                                                                                      2.570 n      32. Acetate                  ;37.
                                     .     =iu rideasF   asN2HO32                       000 0.0         _0.00                 7.39 0;0           ................             0.00 0.1 i                               0000 0:9                      ............   .000            ,.,.
42, Bhder                                          as Br                          0.00                             00                                          0.00                                0.00                                  0.00 3&#xfd;4..chiloride&#xfd;                                    as~ci                          11.9                              12.7       _         _                     1112.7                                                                      20 i__
35, chort                                         asC 1cio 3 -- _                    000__00                                           __                         0                                 0;00                                  0100
                ,;.::4 a t : .:...                        .lt
                                                      ..... N. * -------
                                                             ..            -.           1.3 .. .. ...... . . .. ....   .                 . .......... . . ... .                                                                       ------   0-8 1
: 43. chrshoate 0                                     as, c0 4
     .37. 4Fluoride
              . ..........                               as F =
asC~z                         9.09
                                                                                          .0 __               _           0.00 0.00 0.11 0.00000 0:09                                  0.10
                                                                                                                                                                                                                      ...0.00
     .38. Format*        e-        o                     a      --   - ---  --. - - - 11                      o           00-o_                      o0 _ - I .o0                                            0.                    --              00                                                                                                                                                                                                                                               ---
: 39. Florate: 4~~~~0.MoydtasO                       as SHO                         0.00
                                                                                       -2 00             __
0490 0009                                       0.00 0.00 0:0 0.5 020.0         001___001 4
                 !-'-46:,
                       . . P. ho_
_--------  (R. y,"..
                                        . ........... ..... P . ,.. ........
                                                                          -I......
                                                                              ........ ...                  . .     .   . .....           .........             .a                                                         . .
      -41. Nitrat.                   _                   as NO,                         1.35                              14                                         19                                  091                                   0081 42 itieas                                NO 2                       000                              0.06 __                                     0.00            __                  0.00                                  0;00
: 43. iodumn total)                         _____as
                                                ..           N ---      -----                                             -----      -
44.: Oxalate 51..       ~~~.
Mle uv___D      letTo..noaso                              0 .         ...                  0 9..                                          06               0      ...         000 ...          ......          3,6000 .........
45!.Phospteoto          52 .........           . ...
a... sO s                   .. . . 000 . . . ..     . ...              0.00.. ..... __                             0.00
                                                                                                                                                                      . ..                               0.00                                   Mo___
oy5" 57 T01- tea(olye dBoronas-                        as P0N O
99 1o-o   t -o                           ..... ....... .                         .,qPW6_    ..       .... uo-*. i -,--                '       .--*o ........       ...
           . *Phosphat(                  .rgano)
* a-
                                             - -se-s--e-"                             -                                         Contlnuedo - reve            -"
1 4.Pho~sphorus&#xfd;     56; ~ (ttal)___  ~              ~ as___   1,               _0.00                     0100_ _,000                   _0.06
                                                                                                                                             .................       0,00                0:01    -0.00                   0,00 i:.........
                                                                                                                                                                                                                   *......                     0.00 .....        0.00 0     49. Sulfate,                                       as0                    _.    .21.6                              236 ___                                     247                                 21.5      ___                         20.9
       ~~.50. Sulfur (total)                -~as            S,--                        75                00_                                    00_                   8                    0              4             0.21 _ 819                                0 5i.. Total Anio Mitleqlulvalents                                                 3.508___                           3.1991                                      4.061       ___                      373.385 2.Ammonia                                      asNH3                  ___             _________
: 53. Benzotriaole                                  as c"H N3_               _                   __
: 54. Boron                                          as B                           0.00--           __--            0.00                                        0.00      _      _                  0:00                                _0.00
: 55. Silica                                         a5SlO 2 -                      2.23              0.00           2.00                    5.15                1.91                1.27            1.47            0.61i_                2.69              0.00
: 58. o~dum Nitrite                              a aO                                                                          ___________
    .57. Sodium Sulfite                                  as Na2 SO3
                                - ~                                                                                             Continued on reverse side.

LABORATORY REPORT - WATER ANALYSIS                                                                                      ICustomer No.:                         11001392.
H.O-H Chemicals, Inc. Regarding: Indiana and Michigan Power Company                                                                                                       .Rep6itNo::               as indicated 500NS Vermont St. Locationm Donald C. Cook Ndclear. Plant,                                                                                                          Report Date:
Palatine, IL60067                              1 Cook Place                                                                                                       Analysis Date:
Bridgman, MI                                                                                                       Sample Date:              as indicated 847/358-7400 Fax:i847i358-7082                Treated 5110/Q7                           Control 51171/07                     Treated 5h1707                      Control.5/24/07                  Treated 5/24/07
(#27972)                   1               (#27958)                              (#27958)                        (#27999)                          (#27999)-
                                                                      -Soluble.         Insoluble               Soluble I insoluble                  -Soluble              Insoluble     Soluble     j     Insoluble       Soluble      Insoluble 9.--*Bromate          __    _            as   BrO *         ..... . ....       .... .......           . .         .. . .................................. .........-.......        .. ....
: 60. Chlorite                              as Br      2
* 62*...Dyleoh__
C...-          61.. ylamycloexyimin&
ine.,
__         __     as C102 asC   4H11
                                . ___.................... 3N ....          ... '...         -.... '.                      ...  ..       ...        ..       .    ..       .   ....
e362. Dlethylamine "                      as C4H1 5N
                                                                                                                          ...L ,I                           ..........
i..... . .. ...                      ... .............     .......
p       4     Et yI6 Lam pLn  e t       q          ai                      -- - - -- - - -                                   -------
65 Morphollne                ____         asCAHNO                                                                                             ----
d '66. Dlethyleoe Glycol .                      W b yby   weight n6-.Et-l                             w-gh - --------     -b-                                                  - --       ---                                         --
: 68. P ropylenmeGl         ol*             % yWeght              . .......        .-.-............           -                    .       ....................
     ,69. Aerobic PlateCount ..               org's/ml M 0.        Anaerobic Plate Counts          __  org's/mi                   .        .                .                                                                                ..
i 71.       Fecal Coliforin                     orgf/,100mlI
   . 72. liron Bac6tera__
                                 *,i~~~~~~   **-m._.*T                    .* ..... ;Z;*22*2i`*-]                                   ?T- ---                iLZiL*`*-))*))L]i-i~*-`
                                                                                                                                                           ~~~
                                                                                                                                                        ----     --                                                               22 ...........     ~i r--
  .r732. Mold
                                               'org's/i        _                                                                                                       ---
b   -7.Nitrate         R'eddcds                 o rg'~s/mi                                _
                  *m
  .175. Sfin~e Formersorsmi_       --------
                                                                                     ---   .--  .                                                 ............. ........              1.-- ..        :... . ...-   -* ... ------  2.: .........--
0 76. Sulfate-Reducers __                       ogsm 0- .                       * * ......... ...;; .... . " . .. .~~.--*         .......T " - -I -*...... .
                                                                                  .....                                                         I .'-      ........ .......       ... -*
                                                                                                                                                                           -T-------...  ---------. _                    . . 1 ...
1 3. Total'rganicr arbon                    --. i                                                                                                                                                                                     -p-.
64 S .e a.cy                               ga.........
: o. ....                     -__-----
                                                                 ...... -oa~gn~toe.... .
g s           e e ( -----    --)_-_--- ----           -                        5--                                                                                                       -----  -.                             -             ------
o               so'Vo~fleflid

LABORATORY REPORT - WATER ANALYSIS                                                                                    [customer No;::                         1001392
                                                                                                                                                                                                                                              -Cstoer...
C.nmn~nu NAYSS WATR.
                                                                          ;L             ABOATRYREOR..                                                                                                                                               03.
H-O. -HChemicals,Inc. o.,,....;.,..              di Intliana anr ti Ilirhin.n Pntaunr Indiana and Michi an Power Com an I*nnrt Nn"
                                                                                                                                                                                                         !Reort No&#xfd;:
* indir*t*d as indicAt6d Location:                 Donald C. Cook Nuclear Plant                                                                               IReport Date:.
4Palatine, I                                                IL 60067                                 1 Cook Place                                                                                               JAnalysis Date:
Bridaman. MI                                                                                               [Sample Date:                   as indicated O4Fax:                                  847/358-74000 I                                                                                   T                                 T                               I Fax: 847I358&#xfd;7082                  Control 5/31/07                             Treated 5/31/07                         Control
(#281,00) 6/21/07            Treated 6121/07                  Control 6/28107
(#27999)                                   (#27999)                                                               (#28100)                            (#28129)0 I  1-l!
feh.hW*              QMh.hl.           I.It-hn.ht.           Qhf.hI       I   In-nfihil   I. CWhIn      I  In*aohl.,         QnMhlha I Alln 4hlhl.
0....................                      6
              !AIjajntyAT..                        aSCaqq33                       118                                                                              154                              150
: 2. Alkalinity ("M)                      asCaCO                                                                       120                                                                                                     130 Free Mineral Acidity               as caCO 3 a,       5, "8i Chemical. Oxgen Demand (C.O.D.),                                                                              13:0                                      9.. .
                                                                                                                                                           .............                             7.3                             23:4 chloqroformEtractables                 ____
e&#xb8;            Dissolved Solids,
* il
           ..                                                       ..........                                                                                    213                               210                                224 86 Hardness (Calcium)                 as Caco,                           70                                                                             82                                81
_9. Hardness (Magnesium)                as CaCO.                           41                                         40                                  46                                45                                .46 P      10.. Hardness (Total)_______ as cac                    ..                 !111                                         108                                 128                              127                               "132 r     11. pH                                                      8.1                                          8.4                                      .       31                        8.2                            717 314                               .338
: p.                                                                                                                                                                                                                        ....................
        .13.Specific Gravity                       gmln                                                                                                                                                                                   ...
                                                                                                                                                                                                                                           .3 e
Sseded Solids,_                                                                                                                                                                                  6.5                               :213 r
14.
: 15. Aluminum                              as Al                          00;06 730 0.00                   a05          Abi                   4.14
                                                                                                                                                                                   -1.10           0.01            0.03              0.01              0.67 I-                                            1 t    16, Barium                                 as Ba                                                                                                                                           0.02             0.00                                0.01 0,02 S ................,._.            0.01                 0.02                  0.00            0.02             0.01                                               0.02 I     17. calcium                               'as ca.                        28.0
                                                                        ........    ...  ... 3.37                 ...
27.1 .....                                             8.78           '32.5       I ...0:00 0.00 34:5             19.4 Chromium                            asCr                           0.04                  0.01                 0.03                  0.01            0.01             0.01            0.01                               0.00             0.02 e
19:' Copper                               ascu                                           ........
o...9 .              0.00 0.00                  0.00                 0..0                  0.00            0.00             0.03            0:00            0:02               0.00             0.02
: 20. Iron-                                 as Fe&#xfd;                                                                                          0.12    _....o:0.'03
__              2.39            0.03            0:06               0.00             2.51 0.00
: 21. Lead                                  as'Pb_                                               0.00                                    0.000                             0.000          0.000             0.000           .0.001             0.002 0.000                0.00                                                                                                                       0.00 Lithium                             as Li,                                                                    9.62.                                 0.00         __0:00              0 00             0.00              0.00 0.000                   0.00 Magnesium                           as Mg                          0.00                                       0.00                  0.02                             3.03             11.0 - - -... 0-00 0
: 0.               11.1             5.22
: 26. Manganese                             as.Mn                                               .0.05                                       0.00                             0.11                                               0.03             0.14 N
ce ..........................
                                        .........ads LNI ... ....                                                           0.00
         -23, Nickel
: 28.                                                                                             0:00                                       0.00                                             0 :01 0,00 .............               0.00              0.o0             0.01 25*.Potassium                             as K                                                                      1.83 0.00                                  1.46             9o I.................      1.41                               1.51 0.00                                                                             0.00                                                                0.00 Silvar                              asAAg                                                                                           0.00                             0.10            0.00             0.00 C 28. Sodium                                     as Na                          8.36                                       8&08                                 .7 69                             7.49                               7.51
                                                                                                                                                                                                                                                      '0.01 a 29. Strontium                                  as Sr                          012          ..............
0.00
                                                                                                        ...    ,            0.12                  0.00            0.11             0.0.00          0.11       __0.00                  0.11 Zincl "                             as Zn           "                                                                                                                0:02
: 30. -    ----0 &#xfd;-,' ,     .. .. -&#xfd;- , ..- -.- ......
                                                        -.-..  .                                       0.29                 0;00                  0.24                                             0.01                               0.00             0&#xfd;04 6     31. Total Cation Mllteqqk~lents                                    2.631-                                     2,,546                                 2.924                            2.896:                            1.009 n                                                                               0.00                                       0.00                                  0.00                                             0.0                0.00 5                                                                                                                                                                                                                                    0.00
: 33. Bromide                               as Br                          0.00                                       0.00                                  0:00                             0.00 34: Chlonde Chlorate                            as CI as d!O3 .......                12.5                                        12.4                                 12.0                              12.8 35.
Chromate                            as Cr04           ......
                                                                         ....... _o  o                                                                        .......
                                                                                                                                                                  .i6..
0.00                                       0.09                                  0 00                           . 0.00 Fluoride.                           asF                                                                                                                                              .0--08
: 37.                                                                                                         ...............
                                                                                                                           -..
* 0.07c
: 38. Formate                                as CHO 2                       0~o    00                                                                                                        .0.00                    .........
                                                                                                                                                                                                                                   .:. 29 Glycoate" Mol        t .s as C2 H3O3 a-**e MoO.                                                        ............ ;.                        .............
00 0.00 40:                                                                       0.01                                       0.01                                  0.00 0:00                              0.00
: 41. Nitrate                                as  NO3                        0.92                                       0.87                                                                   '0.00                              0.71
                                                                                                                                                                .0*00o
: 42. Nitrite                                as  NO2                        0.00
: 43. Nitrogen*(total)                       as  N                      ........
zo                                S....
                                                                                                                      ........ 0....6
                                                                                                                                   .0=
0............                                                                                 ----       O9 21--
: 44. Oxalate                                as  CO 4 ..                                                                                                     0.00                              0,0.0 0:00                                        0.00                                                                                                      O.O7 0,00
      -45:&#xfd; Phosphate (ortho)                     as  P0 4                                                                                                       .0.50                              0.52   ------- ---
,A     448: Phosphate'(organo)
_~ !APhosp-e(oy.).,                 POd4 as PO n     47.                                                                                                                                                                                                 ............
6 *.
Ph'osphorus (total),               'as P                           0.00                                       0.00                                                                                                                        0.1:!6 48:                                                                                                                                        0.00            0.00             0.11             0.00            0.07
.0     49. Sulfate         ____                a 0                                                                       21.2                                                                    21.4                       ...............
                                                                                                                                                                                                                                                   .20.216 n                                                                                                     0.30                                                                                         7.01 S     50. Sulfuir (total)                     as S                                                                                             0.50           7.03               1.6                                                               0V96
: 51. Total Anion Milleauivalenfts                                   3:223                                      3.252                                  3.948                            3.889         .. ..
                                                                                                                                                                                                                  . --             3.502 52:    Ammonia                             as NH.
: 53. Benzotrlazole                          as C6H9 N3 ,
0.00                                                                             0.313             5_22_ ,         0.00                              0.00 1:18                                                                                                     2.60
: 55. Silica        ,as                       S102                       1.5E                  3.13                                       0.38            3.13j             5.221           2.34            0171                                439 56-SoduNitrite                             as NaNOi, 57: Sodium Sulfite,                        as NaSO3
: 58. Tolvltriazole;                         as CH*N,,
Ailoto ;pt  P  mPan PIwn    oras lindkm                                                                               Continued on reverse side.

LABORATORY REPORT - WATER ANALYSIS.                                                       Icustomer No.:                  1001392 Report N0.:           ,aS&deg;indicate*
HO-H Chemicals, Inc.         Regardlng!: Indiana and MichigAn.Power Company                                                        IReport     No.: -      as'indicated 500S. Vermont'St I .*;              ...
1.s on:
I'rn-l oca
                                                                                        -, I'
                                                                                                    ,*b.Kh1 JtJE V
                                                                                                               .-lra DlInM
                                                                                                                   .4~d0 .11 0.
E
                                                                                                                                                                       -4  M.Cm; Palatine, IL,60067                     1 Cook Place.                                                                           Analvsis Date:
Bridgman, MI                                                                            sampie Date:           as indicated 847/358-7400 Fax: 847/358-7082           Control 531/07'                    Treateds5/31/07,                  Contro0 6/21/07'`    Treated  6/21/07
(#28100),
Control  6/28/07
(#28129)
(#27999)                          (#27999)                          (#28100)
Soluble    I Insoluble              Soluble    I Insoluble,          Soluble    Insoluble7 Soluble     Insoluble     Soluble I Insoluble I ____           ____        +/-                                                   I ____        ____
i59. Bfromate                       as Br03 C
     .61.'.
0.0 Cyclohexyiamine                6 asCsH   13N                  ...................
: 62. Diethyiamine* .                 as C4HN p,
0-
     ~63. Diethylaminoethanol*           asCeHisNO                      .............
Ul    64. Ethylamnine*                  as C2H7 N_
as C;4H5NO&#xfd;
: 65. MorpLhllne=!
n                            __                                        ........ . .........
d            iethyLeneGlyco               % by weight 67.- gEtylene Glyoil               %.byw*ight Propylene Glycor               3/4 by.,weight 1-69; Aerobic Plate Counts            otrg's/ml                                                                     -I M      70. Anaerobic;Plate   C'ount        org's/mI
: 71. Fecal Coliform                  org's/i00ml C     72 Iron Bacteria-r.' 73' Mlold----        ..         . org's/mi 0
b          Niitrate Reducers    .         org's/mI     .............   ..............
9     75' Slime Formers                   org's/mi 0     76 Sulfate.Reducers                 ogsm I. 77. Total Coliform                  org'siOOml
: 78. YeaSt                          ogs/hin RI                                                                            . . ...... 00
            @esidue.by Evaporation Volatile Solidst
,a ,'81. Sstem Capaci-                  gal. ----
: 82. .Pro.lonate        ___          as C3 502 &#xfd;                                                0.00                                                                           0.0c
: 83. TotIalOrganic Carbon'        ___
Ttal raicNtogn                      __
2.5 w15

LABORATORY REPORT - WATER ANALYSIS                                                                           jCustomer.No.:                     01001392&#xfd; H-O.H Chemicals,,Inc. Regarding: Indiana and Michigan Power Company                                                                                             Report No.:            as indicated 500 S. Vermont St. Locatiorn: Donald C. Cook Nuclear Plant                                                                                              Report Date:'
Palatine, IL 60067                              1 Cook Place                                                                                           Analysis Date:
Bridgman; Ml                            -           "                                                   Sample Date:           as indicated 847/358-7400 Fax: 847/358-7082               Treated 6/28/07                      Control 715/07                       Treated 7/5/07                Control 7/i12107                Treated 7 2/07
(#28.129)                            (#27190)                           (#27190)-                       (#27190)                       (#27190)
                                                                                  *Phh*,I   InI,,hthm Q 1-hle, I I solubt - I I   ~
Q I ble l=    IIen~l I I SOWN Ql,   hL*
iqbl&#xfd; I
I Ie   h=i 1-11-ki-
                                                                                                                                                                                 *nh IhlA "        Inenhhl*      i*ni,,hl, cduhl I I,~ni, Jhlo 1 1 oluble Alkalinity "ac03                                                        o                                     0                                                                 0                           10 2_ Alkaln!*(ty"*M.                             CaCO3 . .
a2~5"                        126                                  134                                                               :134
          ,3 Alkalinity     (iOH) i=cav               as CaCOj w         4. Free Mineral Acidity                     as CaCO 3 _
         '5                                                                                                                                                                                                              6.6 Chemical Oxygeni Deriand (c.O.D-),                                  15.6                                                                                                     6 .9 1 a
Chloroform Extractables
:219                                                                                              208 re
: 7. Dissolved Solid 84 2141 Hardness (Calcium)                      asCaCO5 .                                                            84                               81                              831                           .81 Hardness (M4gnesl)                 .. as CaCO 3                        45                                  44                                 44                              44                            45
                                                                                  .131                                 "128                               125                              127                          1261 pH                                                                                                                              8:1                              7;8                             8&2 12,  SSpecifle-ond uctanc~e               "rmhos*                      :31 327                                310                      .. .. 318_                           307 e0          Spendidic        "Oviy                     m
: 14.                                                                                                                                                               2.0                            245                            8.0 002                                 174 Aluminum                                es Al                                                           0.01               1.78            0.02           0.03              0.01           2.00          0.01           .0.09
                                                                                               .......o.o....                                            0 02
: 16. Barium                                    as.Ba                     0.02                                 0.02              0.01                                              0.02           0.02          0.02            0.00 1.*52 Calcium                                 as Ca                      33.7                          -33.4                          .....      *3             0.97              3.3.1 17.
Chromium.                               asVC                                           0.01 0.060         -0.00
                                                                                                             .........   -9'             001             0.00 0.01 0.00 0.00                         0.00            0.003
                                          .ro... .asL Fe...                                                         0.00                2.73             0:00
        '20.                                                                                  _0:12 10E0              0.01                         0.00
: 28.                                                                    0.000                                  0.00                              0.000        *0.000                              0.01          0.00 Lead                                    as Pb                                         0.000             0.00            10.002            o.00                          0..oo                                            0.01 0 00 Lithium.                                as Li                      0.00 ........... 6.'66                                 0.00                            0.00C             0.00           0.00                          0.00 0:00
                                                                                 .,11.2                                10.8             3.87             10.8                             110.7 Maqg!neim            __s                    Mg 251                                                                                           0.00
                                                                                                                      .o,o              0.18             0.0...0.0
                                                                                                                                                    ...........                           0.00                                         001 23,. Nickel                                  asNi                ... 0.00
                                                                           . ....... ...                              0.00         ...... `-11                                                           0.00C         0 00 Potasiumas K,                                                                                1.43                               0-00                             1,34                         10.81
         .... Silver                                  asAg                       0.00 0.00                                .0.00              0.01                                              0.00 28,                                                                                                           7:12                               01.3 Sodium'                                 as Na                      7.23                0.00                                               2.829                            ,6:82 Strontium                               as Sr                                                                             0.05                            0.003                            0.02          01.99           0.00 a     29.                                                                                                                                                                               0.11 30; Zinc                                      as.zn                       1.21               0 34       ......P
__0.01 : 0,9                              0.00           0.01                             0.05                          0.09 3-1,T talCatio M equvalents ............                               2.950&#xfd;                                                                                              ...... :0-_o                     2:839 I,
: 32. Acetate:                               -asC 2 302 6.0.0                                                                   0.11                               20.0               S.........
                                                                                                                                                                                                                    .2.. =
33., Bromide-                                 as Br
:12.2 Chloride                                asCi*                                                           11.9                               120                              1220                         0.00 Chlorate. ....                          a                                                                                                  0.01
                                                                                                                  . 0.00  o7                            S0oo
       .38.: Chromate                                 as Cr0 4 .                                                                                         0......
0:09                            0.00 0.00 Fluoride                                as F                                                      . 000.......*-                           0.00 0.00
: 37. Formate          _as                          CHO,                                                  .....0..
000......6 6006 0.00 Molybdat           o                    as Mo4                                                                                                                                                           0:00 2.68                               1.10                             a0.0 Goyalate Nitrate                                'as as C 2HO NO4    3 0.09
                                                                                    . .og 0:00                                                                                                     1.35                         1112 438 02.08 trogn                                as N                       0.00
                                                                                          ......,:    . . . I......         03                           0.00 0.00 odiua         rte                      as.C 042     ..
: 46.                                                                                                           0.....
00; A
P~hosphaite eNiotritezola (o&#xfd;rgano) _             a   P042 N-as NOH as P                                           0.05             0...
00_.3          0.I1                                                                                           0:03 n            1---......Pophorus:(tota.lL
                                                    .as SO4                               .......... o..6                                                22.3                             22:3                         22.7
: 52. Sulfate                                                                                                                                                     0;84 0
Sulfur (tqo&#xfd;tl).                        asS                                            0.60                                1.08                                            *68C           .1.23          6.83
: 53.                                                           ......... 42                                                                   *3.586
: 48. T~otal Anion Miliequivalents                                                                                                                                            3,5653                        3.509 457. Ammniaa                                      NHA.
485.Benzotniazole                             as C56H5NL 3 0.009                                0.00                               0.o00                                                         2.146 0.00 54..
          -              Boroni                              2--                 2.-14                                6.62           . 7:54              2.55           1 .99             :433           8.27                          0.98 Sodium Nitrite        ___               asNaN--
Sodium Sulfite                          as NaSOi Totvltriazole                           as C,H.N;
 -           M4ai =pi pfl~ p~t$pmna~on~
;W-          AHd8t.-P1PH1npw*W.M..Wnd-t"                                                                                    Uonutnued on, reverse sio.

LABORATORY REPORT- WATER ANALYSIS.                                  ICustomer      No.:               1001392 H.'O..H Chemicals, Inc. Regarding: Indiana and'Michigah Power Company                                            "ReportNW:              as indicated 500 S. Vermont St. Location: DonaldC. Cook Nuclear Plant                                                     Report Date:
Palatine, IL 60067,                        1 Cook Place                                                     Analysis Date:
Bridgman, Ml                                     ,,,             Sample Date:          asindicated B471358-7400 Fax: 847/358-7082            Treated;6/28/07             Control 7/5/07       Treated 7/5/07      Control 7/1*2/07           Treated7/112107
                                                                        .(#28129)i              (#27190)            ,(#27190)              (#27190)                  (#27190)
                                                                'Ra{Uthe.;      tnsdubie                                               ,l,,hln 1             I ~aI~hI~        In,,nI,,hk.
I  I      .                                               i   -- --          Insoluble  Soluble I  16Wtsble I Soluble-1 Insoluble -Sol.ble&#xfd; I Insoluble I Soluble      I Insoluble' iBromate,                           as Br0 3_
C
&#xfd;6          Chlorite                           as clo2
                                               !asC H1 3N rin 60.            pl*;
i ne. , ........
P&#xfd;                                            as Ct1-115N 61.
0*
U-                                          _as C2H7N Diethylene lycol*                  a~s  CAHNO
: d. 66.                                         %by weight 67,                                      %,by weight
: 68. Propylene Glyco-.                                                      .%bPyyweight Anaerobic Plate Coun A     71,      Fecal~conform                    org's/mt M
72    Iron Bacteria, 73.'  Mold                               org'simi
: 14. Nitrate &#xfd;Reducer Is 7&. Slime Formers' 0          Sulfate Redcr                      org's/IOml Total Coliform%
ao                                             org's/ 0m[
R'esidue byEVapraoi
           ".-ui         o ............
                  . Solids.
IVolatile B-80.
propionate          ..                      5AL             0.00                  -";0.0o                0o00                  0.00                      0.00 TotalO rganic, Carbon:
           .Total Orgaicj Nltogn&#xfd;_
Mexel (A-432)                                                   3.0                                        2.5                                             2.5
                                                               - .-. . . . .-11 0    ADS d,  -   M WtonWa ,mr  lon ofn Ind,*atsd                                                                                            a~naivsbv Gas Chromstaaranl.
k17

LABORATORY REPORT - WATER ANALYSIS                                                                                      Customer N&:`                            1001392 H-O-H Chemicals, Inc. Regarding: ndiana and Michigan Power Company                                                                                                                Report No.:,                   as indicated 500 S. Vermont St. Locatlon: Donald C. Cok Nuclear Plant                                                                                                                   Report Date:
Palatine, IL 600671                                     1 Cook Place                      ,Analysis                                                                                      Date:
Bridgman, MI                     "                                                         -                     Sample Date:                   as.indicated 847/358-7400 Fax: 847/358J7082                       Control 7/19/07
                                                                                          ...#28250)
Treated 7/19/07
(#28250)
Control 7/25/07
(#28250) 1j    Treated 7/25/07
(#28250).
Control8/207
(#28301)
Soluble       I Insoluble                        I               esolublS l uSolub                 le I        olubl             S ule  i      I  Insoluble          Solublei I Insoluble
: 1. Alkalinity (',") _                              as caCO3                             0                               112                       "               012 0             .          .   ..                                              0 2.Alkalinity ("M)._                              as CaCO,                         130                                 128                                    132                                 128                                    130
       ~~ ~ ~
a Ch mi a Oxy g en D e11:
Aklnt(OH)eluidl asCaCO 3 ma nd :( .....  . --0)                            , .....        ,1.            8 I ... ..... .... ,-............... 5           &deg;'                           *
                                                                                                                                                                                                                         . . .... .. ---- --------  ( -..
Fre Min1eral' A idity                         as CaCO 2         _    _      _    _                       -             - -------                 -                                                                         ...........
a     5: ChemicalOxyen Demand- (COD.)                                                       806.____815                                                                                                        4    _10:2
: 6. Chlorofoim Extractables                                                           2
                         ~
:pS~ c~ic1 r. vi* _.. ..... ....                g...l.
er    11. Dispelved
: 14.     )H'             Solids "_.                         _1             8:1                       88021   8:4                             42009 8.0                                       84            8               2     8.0                             8 o     12:. specificC~onductance                        . p.mhos ....                      -305                               ,312                                    311                                   309 ....--             _               309......
r I17.      CalciumT t), .
: 15. Aldminume                                        as asACCaO                          1231 0801           08                   '2152 0.01               00 005                   125-001               808 1.10            -02 31                  000 04           .0011204                   3 08
    --18. Barium                 -00                       asaa _              ..-        002............
2 '---0 001.00.000 ........                                          002               001                002                 000                0.02 ...            01 e     18. Chromium_                          _     .0.00asOr                              0.00            0.00
                                                                                                           .000
                                                                                                         .0.00               000                000                  0.00                                 0-00                                                      0.0
: 24.      Manganese                                                         .0             .8               00                   0 4.sM                 .0              012                009                   200              00                 09
: 21. Lead                                             -as: Pb                    .0.0*00             0.000      _       0000 00.00                              0.000          0.             000                       0000 000                00    ... 00.
23        agnesp  c                                        Mg                         10.            1.                 1                  0(0            .            .    .............
34-          .106                  000                   07         -        3
: 25. Noikel                                            as NiA                         0.00         000                                      0.00                 00                0.00               000                000                 0.00 Copperc                                       a Cu~                          0.02           0.04                0.010                0                  01                03                   0                   0                   0               0.00 0
: 28. n Potassium
: 27. Sliver                                           :_as  K    ......... .....................
1.34                    ...     ...1 ................
30             00             .......1.28 0           "60                   0.00 1l.23                                  0.00 1-"
                                                                                                                                                                                                                                               '30'---:             0.00
                                                         .aAg                             000.                               0.00               00 0                 00.               000                00.00               000 0               '0.00                03.
n 2. Aetate.1.-1.                                       as.P2 3 2                     U0.0           010                 000010                 0               6,000                                 900_0_00 C 28. Sodium                                                                             3 r            8.227.09 880        36.                0.0                  8.53                                6050 3                                     850 a    29. strontium             .as                             sr                         0.01           0.01                0.10               0:00                 010               001                0G10                0.00               0110               0.00
* i~~~ioi-*
                                     . . .... [e....     ;~~~............... ......       __.o0                                                                      o-o                              .----..........                            0o ------           , ..
33.
2       Bromide                                      asBr                            0.000                                                                      0 O0000           00;00              000 o                                      000
: 23. GPyoiate
: 39. ChlorMae                   _
asC as C1!2    O                   0004 0.00                  5             1.300 0.,00                                   0006 0.00         .3                 4 003 10                      0
                                                                                                                                                                                                                                    .00         000 0100 ..                 33
: 37. Fluoridel                                        asFN                          .0.05                                0.05                                    0.05                 .0.07.0         0.05                                                        .
: a. Formale 45.,   Zin
                                               .o as Pn&#xfd;0,200                             o0             _ ---....._...............o.11.1.......I 00                  000 1 0 000.1 01,                    ..............
000 00                  0 .. .................
000o-oo 000    .." ..................        00 ?................
00002 4
: 40. Solvere       al~o g a o                         as as.MgP... ---------- _-        000
_----_                           ...                    _                       000 00000                                000                                   000 4-2.Nirte              --           ... .000 00       . asNo
                                                   ......                                                                                                            0.00                                000                                    000 43.Nitrogen (.ta)                               .. asN.                                                                                             . ..
    '42. OXalate                                  -as          CO 4                         000
: 1) .           V                     1          .00, ...      ..        010                                  00                  0.-000             0.00           ...
: 53. enzortraoe&#xfd;                                      as C105 3                                                          0_0__6 A 49 Ph2s7hat62(oly)                                      as PO8
                                             ........................            -. - -0.00-----                             0-00_                                   0-0                                         - - --------- 2 537,Silurica                                         as.l0                            03059         36505                                                                         53                     3 5i6               4             40.0711 8            !      ,
: 56. ChodimaNtrte                                     as CrO 2                                       _                               _4_
I _48. phosphorus;(total).. .......asP                                - . .........        ?.000 '':            3.......003  00.00(
                                                                                                                                .;9......                 ..... 00?1                   00........
:5 ........ 0091                 000.....;;/       000
                                                                                                                                                                                                                                                 *: ' -' 003~i-o 49. Sulfate.                                            asSo4 _                        204                                 195                                     20.0                                2008
~50.;~if~*total)                                          asContinue                                                                                                                                                                                 0
                                                                                                                                                                                                                                                   .020 e~ae~p~nsn,~'das                                                  741~00                              720                0.00 onorevers     ....7.32 side           000               73o                                    6.79 .000  -
: 51. Total Anion     in Mil...........le...............                                ;5quive..............lent.........
3504...                            3345....              ......            3.518 .......................
M                        341O..................................3:494 I                 i ...
52a Ammlornia                                        as NH3                           11A                               1                                                                                    .4       ...                .3
: 54. Boron                                            as.                                                                000                0.00
_                   0000,                                                                      00 357Sodium Sulfite                                    as Na 2 0O. 3                                                 -I

LABORATORY REPORT - WATER ANALYSIS'                                                    ICustomer No.:.               1001392 H.O-H Chemicals, 1ihC. Rdgarding: Indiana and Michigan.Power Comnpany,                                                                    Report No.::         as-indicated 500tS. Veirmont St&#xfd; Location: Donald.C. Cook Nuclear'Plant                                                                  jReport Date:
Palatinej.L 60.067                         1 Cook-Place                                                                    lAnalysis Date:
Bridgman, Ml                                    _______               ___ISample-Date:                  as-indicated 847/358-74 00T                                                                                                                    I Fax:-B47/358-70 B82 Controlt71910
(#2825,0),
Treated 7/19/07
(#28.250)
Control 712510T-ITreated 7/25107
(#28250)[            (#28250)           J   Control 8/i/07
(#28301).
                                                                         *.oluh~e-
____bt InsoIuhte     SoIhbje           4nStuhla Insoluble___ -
Ilnsc*k*bI II Insduble- I
____bl 5Rokubla
                                                                                                                                                                                      -   -Iu Inscluble 59, Bromate                                       as Br0 3
    ,60. Chlorite                                     asdO 2 '
0 m                 Di~tylamne~__                        as C4H13N P        Diethylaminoethanol                          -as C6H15NO 0'
: 60. Ethylalilne-                                  as C2H7N
*n                Morphflneas C4H-9NO d        Diethylene Glycol                      .     %.         eiglt
'8.
m           hylej                  ,;n-Gly.col*             yWb:   igji
: 73. Piopylene GlycolW                             % by weight
: 74. Aerobic-Plate Count                          orgs/ml M        Aniaerob ic Plate Counto-,~ vm                            __
rc 0-Fecal Coliform
: 72. Iron Bacteria Mold_ _
Nltrate' Reducers org's/1 ooml org's/mt org's.*-mi      _
I-   -
: 69. Slime.Formers                                 oirg'sfrnl I
0*       SUlfate. Reducers                            orgs/m..
                 -rt._o~
Total Coliform . ....................        or   '* o0 or's/1 o_        O0ml=
0    78.
Yeast                                        org'mI
                     .bE..siduepora__pn_
                        ,Re                 tionn..........
                                                    =
Volatile Solids 81.
a:       Systm Capacity&#xfd;__
0;60                                            0 00 0.....
0.
Total Organic Carbon                                                                     I .......... 9                                             -       00......
Total-gai;c Nitroge.
Mi el,(A -43............2).....
                                  ...... ..                        .                                             2:5                                           ....,25*.
                    ----  --------  - -----                                                        I..
G-W         1                         indlwm LABORATORY REPORT . WATER ANALYSIS                                                                            ICustOmer No;:                                   10Imoob6 H-OH Chemicals, IncI Ma Mi InAinn. nnri ft/lihi qn P&#xfd; ..p,(--
                                                                             '~*~'*'~~*       "'~"-'*'~'     ~'   *~      ~                   S
                                                                                                                                                              *'*I.""'l 0.
4~'"-I--,
                                                                                                                                                                                                                -f    Kl" -&#xfd;              'ne     inli&#xfd;citari 500 S. Vermont.St, Location: Donald C. Cook Nuclear Plant                                                                                                 IReport Date:
Palatine, IL60067                               1' Cook Place                                                                                           [Analysis Date:
Bridgman, MI                                                                                            ISample Date:                        as indicated 847/358-7400 Fax: 847/358-70.82                Treated 8/2/07,                        Control 8/8/07 C                                  TreatedS8/8/07                  Cbntrol08/1 5/07                     'treated 8/15/0*7
(#28301),                             (#28301)                             (#28301)                      (#28332)                                  (#28332)
R lh I .Innnh,,htn. I 4:~qn
                                                                                                                      ~ni,hia     I    In~nhihi,.        Rnh,,ili       I In~nhi,hhf     *nnhlhf.t    I    Inf,,ihhln         "nnl,
                                                                                                                                                                                                                                      ,hil h!4 I   ifn~nhihlA 15 insoluble Alkalinity ("P")."as                              CaCO                    16                                    0                                    14                                                                       8 AlkalinitY, (M'                               a-s-Ca-COi,                                                                                           128                          134                                      130 Alklinty("O")(cacunici)as-CaC-O'3-Free Mineral Acidity                         as CaCO3 w       4,,
a           Chemical Oxygn Demand (C.0 D).                                                                                                                      7.0                                                                -   7.2
   ,1 -2L Chloroform Extractables e     7, Dissolved Solids                                                          204                                                                                                                                               206 205                                   202                          212 8; Hardness (Calcium)                              as CaCO3       ..............
                                                                                       &#xfd;_*_4                                  :80                                   178                           85                                        83 Hardness (Magnesium)                         as CaCO 3                                                         43                                   42                            45A                                      45
                                                                                  - ----- 43.
P   '10, Hardness (Total)_                                as CaCO,                  122                                   123                                   120                          '131:                                    128 oH r          Specific Conductance,                         j, mhos___._
3.4:                                8.0                                   8.4                              7.9                                8.3 0   .12.                                                                                                                  308                                   300                          315 13.. Specific Gravity. ......                        gml-t i.11 Suyspended Solids__                                                                           351                                   213                              8.C                               166                                         7.0 ra ,25. Aluminum                                         asAl                     0.02               0.04               0.01                2.55             0.01           o:oe                               2.05                 0.02                  0.08
: 16. Barium                                        as Ba                    0.......o02 0.00               0.02                0.02             0.02           0.01:          0.02                0.02         -0.02                         0.00
: 17. Calcium                                          as Ca                                                          31 :g               17.5           -31.1            OOC            33.9                12.2                -33.1                  0:00 0.00 e    14..
Z'1 copp*r*       Chromiumas Cr                                                 0092 0------
                                                                                .. ...0..,00.            0046               0.00
                                                                                                                                           ---*---o              0:00 O.OC o:0o S                        ..                               as Cu                                                          0.00                                 0.00                         &#xfd;0.00                0.01                 0.00                  0.00 0.000              0:000
: 20. Iron                                             as Fe                                                          0.00                4.04             0.00                         .0.00                3.41                 0.00                  0.20 0.000                                                                                      0.10*                                                                       02000
: 21. Lead                                             as Pb_                                    0.'000             06.000              &#xfd;0.004,         0o.000                        0.000                0.004               0.000
               -Cli.llur . .........
                                  .......... ...........asiL .... .                                      0.00                                                                                                                           0,00 M.ag n~e~sum ............                     as..........
                                                             @  ..Mg.......           105*                                                                                                    ;0 00 0.00               0000               10.4                                                                                   3.04                 10.9
: 24. .Manganese!  .N**_.............................. *as Mn*. ... .                                                  0 00         ..........
0.02      .......to:oo           o r0 o.....
0.01:         ,0.00 0.22
: 25. Nickel_                                           aisN1 0.00 0.00
                                                                                                                                              -A641*
0.00
                                                                                                                                                               '-0.0
                                                                                                                                                                                                                  .0.00                 0.00                  0.00
                                                                                ---   0..00..
                                                                                  . 0:00                                                                                        00Ol Potassium                                     as~K'                                  -- .0-.00
                                                                                              ...........                                                                                                                                                     0.00 Silver                     "           __as        Ag                                                                                                                           1.32                                    1,26 0.04            '0.00            SOC*                              0.00 --------------  0.00               ...600 c           Sodium                                        as Na.
6s6 0........                                              :0.00 636                                                                                                     86.72                                   6.37 a    22.                                                                                                                 0.10 Strontium                                     as Sr                    0.111              o.......
oo*                      ..........                                                  0.11
                                                                                                                                                                                             '!0.01:               0.011                0.11 492 237.Zinc                                              as Zn                                                          OM0 6 67
                                                                                                                                          ..........            &#xfd;0.10o 0
                                                                                                                                                                                *60            0 01:               0.06                                       0.18
   -I" 0          TotlCationw Mlleq'-uivalents&#xfd;.                                      2 753                                  2.756                               2.701,                                                                     00 n          Acetate.asC                                          2H202                                                    0. 00                                                                                                      09.009
   $                                                                                  0.00 Bromide                                       as Br                                                                                                                                                                     0.00 0:00 10.3                                                                                                     10.4                                     10.6
                                                                                                                                                          -10.3                               ,0.00 31:
: 26. Chlorate                                      as:CIO3                                                                                             0:00                         0   00 0.010                                    0.00 Chrornate ...                        ...       as Cr0b4 .                                                     0.00 0.00 Fluoride                                      as F               ----05                                      0,00 0.00                                0.05                                                                   0.05 Formate                                       a CH                                                           0.00                                  ,0.0                                                                  0.00
              !gycolate                                      a&#xfd;s C21-30 3             0.00                                                                      0.00                                                                    0.00
                                                                                                                                                                                              .0.00                                    0.00
: 50.                                                ---
                                                    -------- as MoO4__                0.00                                  0.00                                 0.00                                                     ............ 7900 e                                                          0.00 0.'72                                                                 .. 0:73 Nitrate                                        as.NO.                                                         0.83
: 52.                                                                                                                                                      .ooo 34 Nitrite 350                                                   as NO2                   0.00                                                                                                                                             0.00 Nitrogen..(total)*                             as.........
                                                              .&sect;N~                                                                                                                            -0 00 Oxalate                                       as C 20 4 0.00                                                     ............ oo 0.00
: 37. 0~pao                                                                                                ........... o.o 0_00                                                                                                       0.00 as P0
: 38. Phospshate (porty)              .              as:PO4                                              ............_o0 A    347    Phosphate (organo)                             asP04 n
Phoshorus      (total)                         as                       0.00                                                      0.16                                                               0.12                 0,00                  0.00 30.50 0    42. Sulfate                                        as S04                  21.:3                                  20 2                                20.8                           20........5**                            21.5 n                                                                                  6.74                                                       0.86            6.57                           7..21.*-- 0.61
.5                                                                                                                                                                              0.01                                                   7.11                   0.00
: 44. Total Anion Milleouivalbirts-3:i55                                 3.3 73                             -3:333                                                                  3.407 Ammonia.                                      as NH3 ienzotriazole                                 as 6 5N3&#xfd; Boron                                         as B               -0.00 7m                                                                                                            0.00 0 00 Silica                                        as SiO.                  2.31                                   2.791             10.67            -1.04.           0.62           3:62              10.58                  1.35                  11.72 Sodium .Nitrite                               as'NaNO2 Sodium ,Sulfit                                as Na2SOj Tolyltriazole                                 as C7H6N3
 -            NJISal eaxcpt pHin pars p. nal- . o.       aidi.aed                                                               Continued onmreverse-side.,

LA BORATORY' REPORT                - WATER ANALYSIS                         ICUstomer ,No.:.                1001392 H-OMH Chemicals, Inc. Rbgarding: Indiana.and MichiganPower Company                                                  Report No..          as indicated 500S. Vermont St. Location: Donald C. Cook NuclearPlant                                                        Report Date:&#xfd; Palatine, IL 60067                    i Cook Place                                                           IAnalysis Date:
Bridgman; MI                                                            Sarmple Date:        as indicated 8471358-7400 Control 8/8/07 Fax: 847/358-7082           Treated 8/2/07
(#28301).              &#xfd;(#28301)       [    Treated 8/8/07
(#28301),
Contirol 8/15/07
(#28332)
Treated 8/15I07*
(#28332)
Soluble  I  Insoluble; SolUbl.,            I - ~niutsi~ I In  uin I    ~          I              ,Rnluhle       Inenlirile.
insoObie I Sbiubie I lnsoW&#xfd; I Sol6ble I Insoluble             &#xfd;Soluble        Insoluble Bromate,                             asBrO3 60.
c            Chlorite                             &#xfd;asC10  2.
       ,61. C*yqohexylamin&n" m                                                 as C6H, 1 N
     -62. Diethylarninet                      ,as CIOjN 0.
: 63.          y.a*
                      &#xfd;      t.anql*....
64__..
U            Ethyl1amnin&
Morpholine' as CAH  5NO d     66.                                         % by we ght 8
M
: 67. Ethylene Gl&#xfd;col'
: 69. AerobIc 70; Aerobic Plate Count.
Plate Count
__by~weight org'siml org'st.mrrI.
I      ________________
 -It          Fecal Coliform       ,
c
: 72. Iron Bacteria r'
0*
NitratReducers
:o
      ,75.- Slime&Fo6rers&#xfd;
:76. Sulfate Reducers ....          .         org's/100mI
: 77. Total Colifrm-7*.
a.
              !Residueby. EvaporatIon
: 80. Volatl Solids',
a
                                                    'gal.o
* 0....00 82.. ProM nae                               as C3Hi02 .      0..002                  0:00                                                                      0.00
                                                                                                                                    ........ 0-06                          ----------
Toalrgnc Nitrogen 85s.= Mexe!l LA-432 A04M                      il&#xfd;.       lndh&#xfd;.d                                                                                                    I   -         7.=: -
0
                                                                                                                                                                                             ~

LABORATORY REPORT - WATIER ANALYSIS                                                                   ICustornier No.:                    001 o i92*
HO-H Chemicals, Inc; Regarding: Indiana and Michigan Power Company                                                                                    Report No.:            as indicated 500 S Vermont St. Location: Donald C. Cook Nuclear Plant                                                                                       Repbrt Date:..
Palatine, IL60067                            1 Cbok.Place                                                                                     Analysis Date:.
Bridgman.Ml                                                                             _        Samnple Date:.         as indicated 847/358-7400 Fax: 847/358-7082           Treated 8/23/07                      Control 8/23/07
(#28436)                            (#28436)
                                                                   ,C,.hHt.        I                                                         C               Inetlh *hl*                1-n 1'hhl. QMI,hi,      6'lnll hk!
________________________       ~        ~ ~ ~ ~ __    _    _
                                                                                                        *nl= =ht*
                                                                                                        &#xfd;lub
____2 I.
I___
tnenl=*hl*
                                                                                                                             **'*s "t____
1-f-
: 1.                                                                       :0                                  .ll Alkalinity             )                as CaCO 3 A        RlYainity AM):tS              as CaCo3 w
a         Clienilcal Oxygen DOerand (C.OD:.)                                 8.51 2..
Chloroform Extractables-Dissoived Solid s:
: 8. Hardness (alcum
                                            .     -------  . .               222                                    4E.
r                                                aSCaCO3 92 Hardness(Magnesium)                    as CaCO_       ---           47 10.
Hardness.(TotaI)- ....                 as:CaCO 3                  139                  I               12E 7*9-                               8.1 mhos                    :332                                 30I....
                                                                                                                  . 7 17.
: 15.               Codutnc Specl......
Spcfic,Grdvity                         g/ml ii.............
177 Aluminum                               as.AlL 10.
0.01             1.88 ............. I0.02                -0.00 O0.0
: 12. Barium                                 as Ba                                     '0.01                                  0.00 i3.,  Calcium                                  sCa               ...........
367Z             891
_                                                                      ---32.81
                     .&#xa3;op~~~er:,.,.i.
                           ........... . . ......                                                               0.00 Copper-as                        CU                   0.00              0.01               0.00               0.00 000
                                                                                                             -0.02 Iron                                   as Fe                    0.02                                                     0.07 21, Lead                                   as Pb                 .0:000             0.006                                  0.002 gsium                              as Mg                    0.00 2:34                                   0.00 Manganese                              as Mn                   -6 0~0            '0.16              .0.00 Nickel                              . as Ni                    0.00                                 ~1.l29 O--"oc6
: 26.                                                                                                         0.00 1.20 Potassium                              as. K                     1.27
: 57. Silver 185.                                          asAg                                       0.00
: 28. Sodium 0.0o                                 0.00;               .0               ...................
c                              _____             as Na                    6.82                                 6.74:
: 17. Strontium                                as Sr a                                                                         0.11                                 0.11                0.00 130.
206.
Zinc                                   as'Zn                    0.02              0.04               0.01                0.00 01 21. Total.CationMilllequivalents                                 31108
: 23. Acetate*                               aS:C 2 H302 n  22.                                                                    0.00 a
2s4. B           e.. ..                       asBr                     0.00                      ........0.00g chioridbie        .    -_              as Cl'                    1068 Chlorate                               as C1IO                  0.00 0.00 Chromate "as                               CrO,
: 32. Fluoride                                  as.
528..                                                                                                        10.31
                                                                          .0 03
: 33. Formate                                   asCHO2
_____At4        _as    21-1303
                                                                      ......0.0~0                               0.00 347. Molybdate                               a MO                                                         0.00 Nitrate.                               as NO3 0.00 0.75
: 35. Nittite                      .      .. asNO2 ...                                                     0.00 Nitrogen (total)                       as N
                                                                                                         -- 6o-I Oxalate                                as- 004 "0:09                                 0.00 38.
A         Phosphatee (rOan)                      as PO..
n  40. P~hosphate (orgno)                     a 0 41.- Ph~dsphorus (total)__                    as P:                    0.05              0.0,               0.0c                0.00 Sulfate                                as SO-                  '21.0                             -19.4
.n                                                                        .7.52              7.41               7.02                6.88 a
458.                                                                3.568                                3.183 Ammonia.                               as NH -,
6o70 iezotrlazolq  X T:*L :...............
e             ,      as C6H: 5N3 Boron              ..                  asB:                                              ...............
Silica                                 as S,. 2                 10.2              8E31               3;34                0 40 Sodium Nitrite                         as NaNO-Sodium sulfite                         asiNa6SO3 Tolvitrlazole                          as CH,,N, az pH I An dat4sa=aept l                        a . 7H.... N  L _____           I  _____        J. _____              .1. _____        .L _____       .J. _____        I. _____ I _____        I _____     I.
inpwil perer~in wras ndereed                                                              Continued on reverse~side:

LABORATORY REPORT - WATER-ANALYSIS                                    jCustomer No.:&#xfd;              1001392 H.OAHChemicals, Inc, Regarding: Iridianaz and Michigan Power Company                                                           Report No.:          as indicated 500 S. Vermont St. Location: Donald C. Cook Nuclear Plant                                                               Report Date:
Palatine, IL 60067                                    1 Cook Place                                                    Analysis Date:
Bridgmani MI                                                " " Snmple Date:' as indidat6d 8471358-7400 Fax: 847/358-7082                 Treated 8/23/07               cohtiol811823/07
(#28436)                (#28436)
Soluble: 1 Insoluble          Soluble:  Insoluble Soluble I Insoluble    Soluble:    Insoluble     Soluble    lhsolubl"
          - --                                                                                         4                                          4:                       4 Bromfite,                                        as Br0 3      -3 C
0                   Cycloexylmine                       as C5H13N m      Diethylajine                                     as C 4H    1 1N.                                           ------------
p      Diethylainoethanrt                               as CsH 15N0 u      Ethylarnine                .                     aspc 2 H q-7 n      M-orpho-Line!                                    as C4 N d:     Etheqne Glycot .                                  .b      weight
             .pyjen*yc     G           :ly9
                                          ......... ,          y weight Aerobic Plate Count                              org's/mi Anaerobic. Plate Count                           org's/mt .
Fecal Coliform-                                  org's/100ml Iron Bacteria                                                                                                                                            ....
aMotd                                            org's/mI, C,
Nitrate Reducers-                                or-s/i Slimne Formners&#xfd;                          -        Fr's/mi--
sulfate"Reducers                                 org's/mI.
I.
0 TotalIColifor ...........                             ' o m Yeast,-                     - ---                   g-s/mi Re!sidue jy vaorto Volatile Solids&#xfd;
-'a. ysytem:Gapacitr ....                           gal
        .--P-o.--- ..........
Prbpionale                      ... .....
_asCHj0            .. *  ; 22 ...                                          0.00 Total Organic Carbon                                              ..
T.....  ........ ]
ta( g - Nitrogen.
I,:.,,,,
M M d&#xfd;    .1          Hth .. ft      &#xfd;A-         i&#xfd;dkztd

H-0-H CHEMICALS,                                               INC.
500 SOUTH VERMONT'STREET                                 PALATINE, FAXNO:ILLINOIS 60067 847/358-7400                                                          847/358-7082 DATE:           November 7, 2006 TO:             Tom Armon FROM:           HR. A. Becker

==SUBJECT:==
American Electric Power
               .Donald C. Cook Nuclear Plant 1 Cook Place.
Bridgman, MI Analysis of reverse, osmosis membrane.

==Dear Tom:==
r Attached you will find our laboratory analysis reports pertaining to the above. referenced deposit.
sample(s), our laboratory number 26782.
Ihope this information satisfies your requirements. If any further work Or discussion is needed, please get back to me.
Very truly yours, Hi. A. Becker 1HAB;ld Enclosure, cc- Darius Barkauskas

LABORATORY REPORT - DEPOSIT ANALYSIS                                                                         lCustomer No.:                                  1oo1392 H-0-H Chernicils; Inc.           Regarding: American Electric Power                                                                                      Report No::                                    26782 500 S Vermont St. Lo.cation: Donald C. Cook Nuclear Plant                                                      .                                     Report Date:                                11/7/06 Palatine, IL60067                            1 Cook Place                                                                                           Analysis Date:                              9/25/06 Bridgman, MI                                      -Sample                                                              Date:                       9/21/06 Eax: 847/358-7400 Fouled Reverse                         New Reverse Fax: 847/38-70-82                      Osmosis&#xfd;                           Osmosis,                           A-432 Membrane                            Membrane' Percent; I EQuivalents-             PercentI EuuivaPents             Pere           Eaivalents        Percent       I Eaulvalents          Percent          I Eguivalents
   .1. Aluminum                  -  as A120 3                    0.20           .0.012             .0.31           0o019 2    Barium                       as BaO                       O003            0o000              0.01           6.00o
: 3. Calcium                      asCaO                      51 .70           J1844               ;2.22          0,079 4Chromium                     as.Cr 2O3          -         0.01            0.060               0.02          0.001
: 5. Copper                       as CuO                       0 14            0003                0.06          0.002 e..Iron                          as Fe 20 3                   029             0:0.11             0.34           0.01*3 7, Lead                          as PbO                   . 02           0. ooo               0.00          o.oo0
   &. Lithium                      as LiU2                      0.00                               oo               o0o0-ooo0.00                     ...........
: 9. Magnesium                    as MgO                       4.44       .0.220                  0.15.          0.007 io. Manganese                      as0.01                                       0.000               0.04          0.001 1.. Nickel.as                            Ni                     0:07            0.1002             0.02           0001.-
 -2. Potassium___                 as K20                       0:07            0.002              -0.26.         0.006
: 13. Silica'                        as SiO 2                     0:22            0.007               1.81          0.060
: 14. Silver -- i . Sd um          as a     Ag N a2 00                0.00
                                                          ..... *0*  "5 .......... ,o............
8           0.00 3.......
                                                                                                        .3          0.000
                                                                                                                     .04:*.................... ....... .....   ..... .....       .......   . ..   ........     .....     .................
i.Sodium                       as Na 0                      0:25            6.0008          32.30             1.042
: 16. Strontium                   :as SrO                       0.13            0.002               0.00          0.000
: 17. Tin-       _                   as sno                       0,00 o_                                 0.60           o.ooo 18.. Zinc        .         ..      as ZnO                       0.48            o0012               1.11          0.027 20.,   Boron                        as B40 6                     000                                :o0oo          0.00a
:21. Carbonate                      as CO2                     39.24             11784              0.00 2.Chloride                    :_as Cl                                                            U0.0 2n. Molybdenumr
           *4. teNitra               as MoO 3 .............
:~...........                0.00            0.000         . 0.05     . ....
                                                                                                       .....        0.0o0 .......... .... . . ... .. . . . . . ... .
: 24. Nitrate      ____            As NO 2 -                  __              __
.25. Nitrite                      as NO________
: 28. Phosphate                   ,as P205 :                  :0.14              .0006              1.72          0.073
: 27. Sulfate'                     as SO3                       2.57            0964            59.58             1.488
      . Tlltriazole                  aS, C7HsN:                  __
: 29. __        _     _        _     _          _
30*_.        on Loss lg n iti__                        . ..-..............-
: 31. Undetermined                     ___
 .si,2U n dt e rm n e                                   .....            .. ......          .. ..o-o-                                                                   --------  -               ..............................
2T*taC l                                                   100.00                            100.00. .                             2.4-33.Chloroform            Extractable                           ;2.661                               1.861___                        2.40%.                                  -___                      __
Physical Properties and Appearance-

H-0--H CHEMICALS, INC.
500 SOUTH'VERMONT STREET                                   PALATINE, ILLINOIS 60067 847/358-7400                                                   FAX NO. 847/358-7082:
DATE:           August 30, 2007 TO:             Tom Armon FROM:           H. A. Becker SUBJECT;        Indiana and Michigan Power:Company" Donald C. Cook Nuclear Plant I Cook Place, Bridgman, MI.
Analysis of reverse osmosis memibrane.
Dear Tormn Attached:'you will find our laboratory analysis lreports pertaining to the above referenced deposit sample(s), our laboratory number 28351.
I hope this information satisfies your requirements. If any further workior discussion'is needed, please get back to mre.
Very truly yours, H. A. Becker l-AB:Id Enclosure cc: Darius Barkauskas

LABORATORY REPORT - DEPOSIT ANALYSIS                                                     ICustomer No.:            1001392 H-0-H Chemicals, Inc.                        Regarding: Indiana and Michigan Power Company.                                                         Report Nb.:.             28351 500 S' Vermont St. Location: DonaldC.-:Cook Nuclear Plant                                                                                     Report Date:           8/30/07 Palatine, IL60067                                             1.Cook Place                                                                   Analysis Date:         8/24/07 Bridgman, MI                                                                   Sample:Date:           ;8/23/07 847/358-7400               Reverse Osmosis.
Fax: 847/358-7082                            from Treated Strearm of Test Mexel Riq
                 -~~~~~~~~~~~_____________________                                rulu               I cuuiyarwnis           Percent   1 Zuuivarerns verceni j migivaignis Percent:i  nuuiva;ernt .. ci ai. tuvi
: 1. Aluminum                                           as A620 3                       0.09             0.005
: 2. Barium                                            as BaG                                           '0.o00
: 3. Calcium                                           as Ca-                          0.01
: 4. Chromium                                          as Cr2 0 3 COpper.                                           as CuO                          0.10             0.002 6.
Ilron
        %-.-a.--.  ........ . . . . . .  ..........        as FeAO         3               0.13             0.005 Lead__                                            as, PbO.                       :0.02             0.000 Lithium:                                           as LU                           0.00 20                                      0.000 Magnesium                           ......         as MgO                          2.42&#xfd;            0.120 Manganese*                                        as   MnO                        0,01             0.000 Nickel                                             as   Ni                         0.02                           --------1-.......
T -:.......:-
T "
Potassium                                         as   K20                       :0.03        ----
0.00 1
   .8.. Silica                                             as   SiC 2                      0.19             0,006 Silver                                             as   Ag 2 0                     0.00             0.000 15..
Sodium                                             as. Na2O                                         0.004 I.
Strontium.                                         as SrO                         &#xfd;0.14             0.003 Tin'                                               as SnO                          0.00 Zinc:.                                             asZnO                          -0.03             0 .001 21.
0:000 Boron                                              as B406
                                                                                          .0,0011 0.000 Carbonate:                                         as CO2                       40.11,               1.0o3              -----------
27.
27.
Chloride                                           as Cl Molybdenum-                                        as'MoO 3                        0.00
: 24. Nitrate                                            as NO2
                                                                                ................... .:=
15,.
Nitrite.
as,, NO.
                                                                --,--.---v-v :
        ,Phosph!*ate .............as .P2o0                              5                     0.14          00016 230.
Sulfate                  .as                            SO3                        3.01             0075 T0lyltriazole                                      as CAH N.
Ignition Loss Undetermined                                                                       0:27 100.00 Total flhlnrnf~rm IPvfrnrtf*hlI_
Physical Properties and 1"wide cross-Appearance: section.

H-O-H CHEMICALS, INC.
5b00 SouTH VERMONT *STREET                                     PAL .ATINE. ILLINOIS 60067 847/358-7400                                                         FAX NO. 8471358-7082 TO:             Tom Armon                                            DATE: October 19, 2006                      1001392 FROM:            H. A. Becker

==SUBJECT:==
American ElectricPower Donald C. Cook Nuclear Plant 1 Cook Place, Bridgman, MI Evaluation of corrosion test-coupondata

==Dear Tom:==

Attached you will find our laboratory report pertaining to the above referenced corrosion coupons, our laboratory reference number 26910.
The rate0f corrosion experienced.by a corrosion. coup on is derived through a very precise determination of anyvWeight lossthat may have occurred as a result of exposure of the coupon to system conditions for a period of at least 30 days.
Given thedimensions of the test coupon, its material Of construction,,:and the time of exposure;weighit'loss data may be equated to an average thinning of the coupon.over its entire surface. Coupon corrosion rate data should be evaluated accordingto thefollowing criteria.
.Evaluation            Steel        Stainless   Galvanized,     Aluminum         Copper             Brass Excellent           0.00-0.99      0.00-0.24      z.00-0.49      0.00-0449      0.00-0.24       0.00-0.24 Good                1.00-2. 99     0.25-0149      0.50-0.99      0.50-0.99      0.25-0.49       0.25-0;49 Fair                3.00-4;99      0.50-0.74      1.00-1.99      1.00-1.99      0.50-0.74       0.50-0474 Poor:               5.00-6.99      0.75-1.24      2.00-3199     2.00-3.99       0.75-;1.24      0.75-1e24 Unacceptable       7;00-Over       1.25-Over. 4.00-Over'    4.00-Over       1.25-Over      1.25-Over Corrosion coupon data pertaining to this evaluation may be summarized as follows:
Coupon                         Days                        System      Weight Loss Corrosion Rate No.         Material       Exposed       Treatment        Type           :(Om)           (MPY)          Evaluation
: 1. TB751          Steel            43,          None      OnceThrough         0*6537            8.51        Unacceptable 2'. T-75K          Steel'           43           A-432     Once Through        0.29.89.         .3.89             Fair, I hope that this information satisfies your requirements. If any further laboratory work or discussion is, needed, please get back to me.
Very truly yours; H*A Becker HABIld

H-O-H CHEMN IICALS, I'NC 500 SOUTH VERMONT STREET                                             PALATINE, ILLINOIS 60067 847(1358o74        .                                                              8471358- 7082 NO. ZFX CORROSION TEST- STRIP TYPE' Please complete the'important information below, being sure toinclude your full company name-and address, andthe name`of your, H-O-H representative. Return comp!eted, form with the exposed test strip to our laboratory for determination ofcoirrosion rate. Laboratory data will .be relayed to you throUgh' your sales.representative upon completion.
CUSTOMER IDENTIFICATION,/ INFORMATION Company: American Electric Pbwer Address:' Donald.C; Cook Nuclear Plantq 1 CookiPlace Bridgman, MI Your H-OaH:Sales Representative:          Tom Armon Water Type:                            Condensate                       Open Recirculating Cooling Water                    Closed x       Once Through                     Other.
Treatment,               None Llocatobn in System:&#xfd;    Coupon rack on Mexel test rig installation Dat-T          8/30/06                           Removal Date:          10/12/06 H&#xfd;
                                                   &#xfd;H-0 -LABORATORY          DATA Test StripNo.:             T-751                                     Metal:         Steel DaysExposed:                 -43                            Laboratory No:          269.10 WEIGHTS (in grams)                       Original:       -16.9772 Final:        16.3235 Loss:         0.6537 Mils Penetration p6erYear (MPY):             8.51 CORROSION'DESCRIPTION:
x      Severe                    MOderate'            Slight                  Negligible x      Even                      Uneven               General                 Localized 4.0     Maximum Pit Depth (mils),.

H -O-                 H0iHENMICALS,        I                        INC.
500 SOUTH VERMONT STRE ET                                           PALATINE, ILLINOIS 60067 847/358-7400                                                            FAX NO. 847/358-7082 CORROSION TEST - STRIP TYPE Please complete the important information below, 'being sure to include your fullcompany name and address, and the name ofyour H-O-H representative. Return completed form with the exposed test strip to our laboratory fo&determination of corrosion rate. Laboratory data will be relayed to you throughoyour sales repre-sentative uponmcompletion.
CUSTOMER IDENTIFICATION I INFORMATION Company: American Electric Power Address: Donald C. Cook Nuclear Plaint 1 Cook Place Bridgmani MW Your H-O-H Sales IRepresentative:          Tom Armon Water Type:                            Condensate                       Open Recirculating.
Cooling Water                    Closed x       QOnce Through                    Other Treatment:                A-432 Location in System:&#xfd;      Coupon rackon MeXeltest rig Installation Date:          8/30/06                            Removal Date:        10/12/06 H-O-H LABORATORY DATA Test Strip'No.:            T-75K                                     Metal:         Steel Days Exposed:                43                             Laboratory No.:,       26910:
WEIGHTS (in grams)                       Original:        16.6354 Final:        16.3365 Loss:          0.2989 Mils Penetration per Year (MPY):              3.89 CORROSION DESCRIPTION:
_Severe           x       Moderate             Slight&#xfd;                ,Negligible
_Even             x       Uneven               General             _   Localized 4.0,     Maximum Pit Depth (mils)

H-O-H CHEIFMICALS, INC.
500 SOUTH'VERMONT STREET                                     PALATINE, ILLINOIS&60067
                                 -8471358-7400                                                     FAX NO. 847/3587082' TO::              Tom Armon                                             DATE:' November 7, 2006,                1001392 FROM:*           H1 A. :Becke.r

==SUBJECT:==
American Electric Power DonalddC. Cook Nuclear.: Plant.
1 Cook Place. Bridgman, Ml Evaluation of corrosion test coupon data

==Dear Tom:==

Attached you will find our. laboratory report pertainhing to the above referenced corrosion coupons, our laboratory reference number 27022.
The rateof corrosion experienced by a corrosion couponis derived through a vetyprecise determination..of any weight loss that may haveloccurred as a result'of exposure of the coupon to system conditions for, a period of at'least;30 days.
Given the dimensionsof the test coupon, its;material. of construction, and the time of exposure; weight loss data may be equated to an average thinning of the.*coupon over its entire surface. Coupon corrosion rate data:should be evaluated accordding to the.following criteria.
Evaluation,            Steel         Stainless:  Galvanized       Aluminum           Copper        Brass, 0.00-0.99 Excellent:                          01.00-0,24.   .0.00-0.49       0.00-0.4A        0.00-0;24  0;00-0.24 Good                1,00-2.99,      0.25-49:      0;50-0.99        0.50-0.99        0U25-0.49. 0:25-0.49:
Fair                3.00-4.99.      0.50-0.74:     1,.00-1.99      1.00-1.99        0.50.0.74  0.50-0.74 Poor.               5.00-6.99:      0.75-1.24     2..003.99        2.00-3.99        0.75-1.24. 0.75-1.24 Unacceptable       7.0o-Over        1 .25-Over    4.00-Over       4.00M-Over        1.25-Over  1.25-Over Corrosion1coupon data pertaining to.this evaluation may be' summarized as follows:
Coupon                           Days                         System       Weight Loss Corrosion Rate
         .No:.       Material        Exposed      Treatment          Type.            .(gm) .    (MPY)         Evaluation
: 1. T-75J           Steel             43          A-432       Once Throiugh        0.2947       3.84             Fair,
: 2. T-75L           Steel             43           None       Once Through         0.6093,      7.94        Unacceptable I hope thatfthis information satisfies your requirements. If any further aboratoryiwork or discussion is needed, please get back to me.
Veryy truly yours,.
HA. Becker HAB/Id

H-O-H CHENICAL$,                                                       INC.
500 SOUTH VERMONT STREET                                                PALATINE, ILLINOIS 60067 847/358-7400                                                                FAX NO. 847/358-7082 CORROSION TEST -STRIP TYPE Please complete the important information below, being sure to include yourfull -company name and address, and the name of your H-O-H representative. Return completed form withjthe exposed test strip to ourlaboratory for determination of corrosion rate&#xfd; Laboratorydata will be relayed to you through your sales representative; upon completion.
CUSTOMER IDENTIFICATION I INFORMATION Company: American Electric Power Address: Doriald C. Cook Nuclear Plant:
1 Cook Place Bridgman, MI YouroH-O-H Sales Representative:           Tomn Armono Water Type:                            Condensate                           Open Recirculating
                                       ,Cooling Water                       Closed x       Once, Through                      -Other Treatment:                A-432 Location in System:
Installation, Date:          8/30/06                              Removal Date:         10/12/06; H-O H LABORATORY DATA T.estiStrip No.:&#xfd;          T-75J                                         Metal:.        Steel Days Exposed:                 43                                Labo ratory No.:        27022 WEIGIHTS (0i grams)                      Original:        17"I.117 Final:        16.8870 Loss:          0.2947 Mils Penetration per Year (MPY):               3.84 CORROSION DESCRIPTION:
Severe           X       Moderate    _Slight                             Negligible:
Even             x       Uneven                  General                 Localized 5.0      Maximum Pit Depth (mils)

H-O-H CHEHICALS, INC.
500 SOUTH VERMONT STREET                                              PALATINE, ILLINOIS 60067 847/358-7400                                                              FAX NO. 847/358-7082 CORROSION TEST - STRIP TYPE Please completethe important information below, being sure to include your full company name and addressi and the name of your H-O-H representative. Return completed form with th.e exposed test stripoto otur laboratory for determination*
of corrosion rate. Laboratory data will be relayed'to you through your sales representative upon completion.
CUSTOMER IDENTIFICATION i INFORMATION Company: American Electric Power Address:- Donald C. Cook Nuclear Plant 1 Cook- Place Bridgman, Ml You r.H-O-H Sales Representative:           Tom Armon
-waterType:                            Condensate:                        Open Recirculating Cooling Water.                     Closed x       Once Through                       ._Other .... ..
Treatment:*               None Location in Systemrn installation Date:          8/30/06                              Removal Date:        10/12106 H -0G-    H LABORATORY DATA Test StripNo.:            T-75L                                       Metal:.       Steel Days Exposed:,               43                               Laboratory No;!.       27022 WEIGHTS (in grams)                      Original:            16.8973 Final:           16.2880 Loss:            0.6093 Mils Penretration per Year (MPY):               7.94 CORROSION DESC'RIPTION:
                    *_    Severe           x       Moderate%_         _   Slight       _  _      Negligible x      Even                     Uneven                 General                Localized 8.,0     Maximum Pit Depth (mils) 1<

H-O-H -CHEMICALS, INC.
500 SOUTH VERMONT STREET                                      PALATINE, ILLINOIS 60067T 847635874400.                                                      FAX NO. .847/358-7082 TO:               Tom Armon                                          DATE: January 8, 2007                       10013912 FROM:             H. A. Becker

==SUBJECT:==
American Electric Power Donald-C. Cook Nuclear Plant 1 Cook Place Bridgman, MI Evaluation of corrosion testcoupon data

==Dear Tom:==

Attached yoU will find our laboratory report pertaining to the above referenced Cdrrosion,coupons, our laboratory reference number 27212.
The rate of corrosion.experienced by a corrosion coupon is derived through a very.precise determination of any'weight loss.that may have;occurred as a result~of exposure of the coupon to system conditions for a period of at least 30 days.
Given thedimensions of'the test coupon, its materialof construction, and thetime of exposure; weight loss data may be equated to an average thinningof the coupon over its entire surface; Coupon corrosion rate data should be evaluated according to the following criteria.
Evaluation              Steel        Stainless   Galvanized. Aluminum         Copper             Brass.
Excellent            0.00-0.99.      0.00-0.24     0.00-049'     0.00-0.49      0.00w0.24      i0.000r24 Good                 1.00-2.99       0.25-0.49    0,50-0.99     0.50-0.99       025-0.49       0.25-0.49 Fair                .3.00-4.99      '0.50-0.74     1.00-1.99     1.00-11.99     0.50-0.74       0.50-0174 Poor                 5,00-6.99       0.756-1;24   2.00-3.99     2.00-3.99       0.75-1.24       0.75-1.24 Unacceptable        7.00-Over       1.25-Over     4.00-Over     4.00-Over       1.25-Over      1.25-Over Corrosion coupon data pertaining to this.evaluationnmay be summarized as follows:
Coupon                          Days                       System      Weight Loss Corrosion Rate&#xfd; No.         Material      .Exposed       Treatment        Type,.          (gm)         .(MPY)           Evaluation
: 1. T-80P          Steel             56         A-432     Once Through       0.4109            4.11              Fair
: 2. T-80Q           Steel            56           None:    Once Through       0.3446,           3.45              Fair
: 3. T-80R:         Steel            56          A-432     Once Throug.h      0.6190.           6.19'            Poor
: 4. T-805          Steel            156          None,    Once Through - 0;3594.               3159:             Fair hope that this, informationsatisfies your"requirements. If any further laboratory work or discussion is needed, please, get backto me..
Very trulyyours; H.A. Becker HAB/Id

H-O-                       C H EMIlCA L$, INC.
500 SOUTHVERMONT STREET                                                     PALATINE, ILLINOIS 60067 847/358-7400                                                                        FAX NO. 847/358-7082 CORROSION TEST - STRIP TYPE.
Please. complete the-important. information below,: being.sure to include your full company nameand address, and'the name of your H-O-H representative. Return completed form.with'the exposedtest strip toour laboratory for determination of. orrosion',.rate. Laboratory data will be relayed to you through your sales eepresentative upon completion.
CUSTOMER IDENTIFICATION I INFORMATION Company: American Electric Power.
Address:. DonUald,. Cook Nuclear Plant 1 Cook.Place      Bridgman, MI.
Your H-O-H Sales Representative:          Tom Armon' Water Type:                        ..... Condensate
* Open Recirculating
                             ...._      _  Cooling.,Water                      Closed x          Once Through            _    _      Other.
Treatment:               A-432 Lbcation:in System:      Test, rig.over Unit2 discharge. platforfmf Installation Date:               l0/l 2/06-                           Removal Date:          12A7/06R
                                                       &#xfd;H &#xfd;H LABORATORY DATA Test Stdi No.:                T-80P                                         Metal:         Steel;.
Days Exposed:                  &#xfd;56                                Laboratory No.:         27212 WEIGHTS (in grams)                           Origihal:         17.1763 Final:         16.7654,.
Loss:          0.4109 Mils.Penetration Per Year (MPY):.         .       4.11 CORROSION' DESCRIPTION:
Severe                 xA Moderate                    Slight      ___. ___Negligible Even                  Ix      Uneven                 'General'                 Localized 0.5     Maximum Pit Depth (mils)

H"-O-H CHE'IICAL$, INC.
500 SOUTH VERMONTS'TREET.                                             PALATINE, ILLINOIS 60067.
8471358-7410                                                              FAX NO. 8471358-7082 CORROSION TEST- STRIPTYPE Please complete the important information below, beingesure to include;,your full company name and address, andthe name of your H-O-.H representative,. Return completed form with the exposed test strip to our laboratory for determination of c6rfosion rate. Laboratory data will be relayed to you through your sales representative upon completion.
CUSTOMER IDENTIFICATION /INFORMATION CQmpany: .American"Electric Power Address: Donald 0. Cook.Nuclear.Plant I Cook Place .Bidqman, Ml Your H-O-H Sales Representative,;          Tom Armon Water Type'                      _ _     CondenSate                       Open Recirculating
_Cooling'Water                            Closed x       OnceThrough                       _Other Treatment:'               None Location in.System:       Test rigoverunit2 discharge platform Installation Date:           10/12/06                            Removal Date:         12/7/06R H .Hr LABORATORY DATA Test Strip No.:              T-80Q                                      Metal:'       Steel Days Exposed:                  56                             Laboiratory No!:       27212 WEIGIHTS (ingrams)                                          17.2254 1Original:
Final:        116'.8808 Loss:-         0:3446' Mils'Penetration per Year(MPY):.               3.45 CORROSION DESCRIPTION:l
__ Severei             x       Moderate              Slight .         _     Negligible
                         'Even              x       Uneven                Generali               Localized 2.0       Maximum Pit DepthK(mils)

H-O-H CHEMICALS,                                                     INC.
r I7       o         500 SOUTH VERMO -NT STREET                                            PALATINE,.ILLINOIS 60067, 847/358-7400                                                              FAX NO. 84,7/358-7082 CORROSION TEST - STRIP TYPE Pleasecomplete theimpqrtant information below, beihg sure toinclude your full company name and address, and the, nameof yourH-O-H representative. Return completed form With the exposed test strip to our laboratory for determination of corrosion rater Laboratorydata will be relayed to you through your, sales representative upon completion, CUSTOMER,!DENTIFICATION/ INFORMATION Company: American Electric Power Address: Donald C&#xfd; Cook Nuclear Plant, 1 Cook Place Bridgman, MI Your H-T-H Sales Representative:           Tom Armon Water Type:                            Condensate                        Open Recirculating:
Coolingl Water                    Closed x       Once Through                      Other Tieatment:                A-432 Location in System:       Test rig over unit 2discharge platform Installation Date:           10/12/06     ,                     Removaq Date:         12/7/06 H :. H LABORAORY DATA.
Test Strip No.:,          T,80R                                      Metal:          Steel Days Exposed:-               56                             Labo ratory No;:         272112:
WEIG H-TS (in grams)-                    Original:        17.3501 Final:        16.731.1 Loss:          0.6190 Mils Penetratio0n per Year (MPY):              6.19 CORROSION DESCRiP.TION:
i x       Seve~re                  Moderate,           . Slight      __     _    Negligible'
: x.      Eveni                  _ Uneven                General                 Localized 1,5      Maximum Pit Depth (mils) nn

H-O-H CHEHICALS,                                                       INC.
500 SOUTH VERMONT STREET                                                PALATINE ILLINOIS 60067 8471358-7400                                                                FAX NO. 8471358-7082 CORROSION TEST - STRIP TYPE Please complete the important information below .being sure to include your full company name andaddress, and the name of your H-O-H representative. Return completed form with the:exposed test strip to our laboratory for determination of'corrosion rate. Laboratory data willibe relayed to you through your sales representative upon completion.
                                      'CUSTOMER IDENTIFICATION I INFORMATION Company: American Electric Power Address: Donald C. Cook Nuclear Plant 1.Cook Place Bridgman, MI Your H-O-HoSales Representative:           Tom Armon Water Type:                            %Condensate                         _Open Recirculating
                                  . Cooling Water                -  -_ Closed Other X       Once Through None; Treatment:
None:;
Location .inSystem:       Testrig over unit 2 discharge platform Installation Date:          10/12/06'                            Removal Date:.          12/7/06, H H LABORATORY DATA Test Strip: No::.           T-80S                                        Metal:.        Steel Days Exposed:                .56                              Laboi ratory No.:        27212 WEIGIHTS (in grams)                      original:          16.9964 Final:  .      16.6370 Loss::          0:3594 Mils Penetration per. Year..(MPY):          ,   3.59 CORROSION DESCRIPTION:
                         *_Severe           x       Moderate               Slight                  Negligible
* Even.                x       Uneven                 General                 Lo calized
                                                      .0.5       Maximum. Pit Depth. (mils)
                                                                                                                           \O~~ I

H-O-H CHEMIICALSo, INC.
500 SOUTH VERMONT:STREET                                       PALATINEj ILLINOIS 60067 847/358-7400                                                        FAX .. 84. 35. 082 TO:             Tom ArmoniDarius-Barkauskas                          DATE: September 12, 2007                   1001392
.FROM::          H.A. Becker

==SUBJECT:==
&#xfd;       Indianaiand Michigan, Power Company Donald C. Cook;Nuclear .Plant 1 Cook P!ace Bridgman, Mi Evaluation of'corrosion test coupon data

==Dear T-om.1Daribs":==

Attached you will-find our laborator-.report pertaining to the above referenced corrosion cdupons, our laboratory~reference number 28856.
The rate of corrosion experienced by &#xfd;a corrosion coupon.is derived~through.a very precise determination of'anyweight loss that may have-occurred as a result of exposure' of the coupon to system conditions for a period of at least 30 days.
Given the dimensions ofthetest coupon, its matera of cofistruction,*and the-time of exposure; weight loss data may be equated.to an average thinning of the coupon ovqe itstentire surface. Coupon. corrosion rate data should be evaluated according;to thefllowifn criteria, Evaluation             steei       Stainless. Galvanized      Aluminum         Copper           Brass, Excellent           0.00-0.99      0.00-0.24       0.00-0.49     0.00.0.49       0.00-0.24'     0.00-0.24 Good                1.00-2.99      0:25-0.49i      0.50-0.99     0.50-o.99       0.25-0.49      0.25-0.49.
Fair                3:00-4.99      0.50-0.74       1.00-1.99:    1.0041.99-      0.50-0.74      0:50-0.74 Poor                5.00-6:99      0:754.24        2.006-.99'    2.00-3199       0.75-1.24      075-1.24 Unacceptable       7.00-Over.      1.25-Over       4.00-Over     4:00-Over       1.25-Over     1.25-Over Corrosion coupon data pertaining to this evaluation maybe summarized as follows:
Coupon,                       Days                        System.      Weight'Loss Corrosion Rate No.        Material      Exposed        Treatment.        Type.           (gm)        .(MPY).         Evaluation 1., T-83K         Steel           200W           Mexel     Once Through       0.4594           1.29            Good
   .2; T-83L          Steel           200            None      Once Through       0.4761'          1.33            Good I hope that this informnation Satisfies your requirements. If any furtherl aboratoryWork or discussion is needed, please, get back to&#xfd; me.,
Very truly yours, H.A. Becker HAB/id

H-O--H CHEMICALS, iNC.
50.0.SOUTH VERMONTSTREET                                                          PALATINE,,ILLINOIS 60087 a47/358-7400                                                                          FAX NO. 8471358-7082 CORROSION TEST-- STRIP TYPE.
Please complete the important information below, being sure to includeyour full company name and address', and the name of your.H-O-H.representatiVe. Return completed form with the exposed test strip to our laboratory for determination of corrosion rate. Laboratory datawill be relayed.to you through your sales representative uponcompletion.
CUSTOMER IDENTIFICATION 1/INFORMATION C&#xfd;ompany: Indiana and Michigan Power Company Address: Donald C, Cook NUclearPlant 1 Cook Place Bridqman, Ml Your H-O-H Sales Representative::           Tom Armon/Darius Barkauskas Water Type:               __        _Condensate.                                    _ _Open Recirculating"
                              "__     _  Cooling Water                            -_   Closed X      Once Through                                  Other       _
Treatment:                 Mexel Location in System.        Test rig over unit.2 dischargeplatform Installation Date:.            2/4107                                       Removal Date:           8/23/b7R H H-LABORATORY DATA Test Strip No.:             T-83K                                                   Metal:         Steel Days Exposed:                  200                                       Labo ratory No.:.    '    28856 WEIGP ITS (in grams).                       Original:            17.3728 FinaF,          16.9134 Loss:            0.4594 Mils Penetration per Year (MPY):                       1.29 CORROSION DESCRIPTION:
_._,,,___Severe                 x       Mbderate.,     ._.....        Slight                 Negligible Even                   X      "Uneven                 "      General           '    Localized 0.5        Maximum Pit Depth (mils)
                                                                                                                                      ~9C)

H-O-H CHEIM4ICAL$, INC.
500 SOUTH VERMONT STREET                                                PALATINE ILLUNOIS 60067 8471358-7400                                                                FAX NO. 8471358-7082 CORROSION TEST - STRIP TYPE!
Please complete the important information below, being sure to include your fiull c6mpany name and address, and the name of your H;O-HHrepresentative. Return completed form With the exposed test strip to our laboratory for determinatibn of corrosion rate. Laboratory data will be relayed to'you through your sales representative upon completion.
CUSTOMER IDENTIFICATiON I INFORMATION
,Company- Indiana and Michigan Power Company-Address: Donald C. CookNuclear Plant 1 Cook, Place Bridgman, MI Yur&1H-O-H Sales Representative-          Tom ArmonDarius Barkauskas:
WaterType:                            Condensate                           Open Recirculating Cooling Water                        Closed x       Once Through                         Other.
Treatment:;              None Location in Systerni     Test rig over uniit 2 discharge platform Installation Date:           214/07                               Removal. Date:         8123/07 H -0a- H LABORATORY"DATA Test Strip No.:           T-83L                                         Metal:.        Steel Days Exposod.               200   .                            Labo ratory No.:     . 28856 VVEIG"HTS (in grams)                    Original:          1.7.2034 Final:.        1617273 Loss:         .,0,4761 Mils: Penetration per Year (MPY):                0.50 CORROSION DESCRIPTION:
Severe:                    Moderate           -Slight                    Negligible Even                       Uneven                 General      *____Localized Maximdm Pit Depth (*is) t

APPENDIX 8 Assessment Number:, SA-2003-REA-003-QH-i Assessment.Dates:12/15/03.to 01/25104, Condition Report: CRT03344013 Assessment Topic:* Zebra MusselrMonitoring and Control Program Lead Assessor: Eric Mallen Peer Evaluator: Richard F. Green, Nine Mile Point Nuclear Station, Reviewed B       y/                        Oq        Apporval          ?IAhAIjui           1 Lead Assessor      I Date                          Responsible Managemerft/Dae" Executive Summary&#xfd; introduction!
The Zebra Mussel Monitodng and Control program Is dictated by the requirements described within AEPO:NRC: i104, Generic Letter 89-13; Service Water SystemProblem Response Action hem .:Contiol f ServiceWaterSystem Biofouling. The plant requirements currentiy exist as,
 .commitments within'the NRC Commitment Database and are implemented by ENVI-8913 Rev. 3.
Zebra Mussel Monitoring and Control Program. This program document satisfies the objectives of Generic Letter 89-13..
One crlticalattdbute of the program document was reviewedIn -this self-assessment. This attribute being, maintaining the6intake tunnel zebrarmussel infestations to <2 inches to mihimize dumpsbreakinng off and challenging the traveling screens and systems downstream. A preventivetreatment strategy using adaily bidcide applicatitn specified in Step 4.7.,.Chenica-Control Methods, of E'VI-8913,.Zebra Mussel Monitorngand ControlProgram was-employed!n 2003'to control zebra mussel infestation In the Intake tunnels. The self-assessment will determinetihe efficacy'of the'preventive treatment strategy In Its being able to control zebra mussel n6,festations In the Intake tunnels.
Results in*atneral terms,
'The objectives of the self-assessment were achieved. Mr. Richard Green. a peer evaluatorfrom the Constellation Energy Nine Mile Point Nuclear Plant In charge of their zebra mussel monitoring and control program assisted In the self-assessment. IntervIews were held-with Ms. Carol Grandholm, a contract zebra mussel monltoringtechnician, an d Mr. WiliamrJung, a contract chemicalapplications engineer for GEBetz. Reviews of Reqiuest for Proposals and chemical vendor responses, letters of request for blocide approval and responses from the MDEQ, the application procedure, settlement monitoring system and data, chemical residual blo-box'and unit discharge data, and personnel interviews were valuable In assessing the crilical attWbute.
Primary Chal*egnes Results ofAdiVing inspectionsof the North and Center Intaketunnels reveaIled that zebra mussel infestations were S2 Inches on the tunnel walls. From review oftheblo-box settlement data and discussions, this infestation level.was kept In check for the most part via tunnel flow (6-7 ftJsec) as opposed to the chemical treatment- Results from the preventivebiocidetreatments were not as favorable as expected due to; 1) Very restrictive MDEQ discharge limits (706 ppb), 2) Low system demand that was available to reducethe discharge concentration In the unit discharges.
and 3) Inadequate dilution flow due to the intake fbrebay design not providing a perfect 2/3 reduction In concentration before the effluents are discharged. Despite these restrictions to the preventive treatment regime, zebra mussel sloughage from the IntaketunnelsIn 2003 was NgpeIlo~f P,
: c '

managed by the traveling screens without impacts to components downstream..The plant should continue to maintain an aggressive posture In controlling zebra mussels In the intake tunnels to prevent under-deposit corrosion of the tunnel wails, and prevent an event that occurred at the Palisades Plant(OE #11308, 611612000) where an unexplained die-off of mussels from the plant's intake tunnels occurred resulting in large'clumps of mussels being swept into theintake bay and challenging the traveling screens.
Assessment Strenoths None Assessment Findings and Prescdbed Corrective Actions, None Recommendations-and Proposed Actions
: 1) The preventive treatment program was Implemented as designed. There are no findings but
: a. recommendation to review this assessment with peers and vendors to develop a more effective chemical preventive treatment program, mechanical cleaning, or revisit targeted shock treatments to the intake tunnels.
2), The peer evaluator noted that the blocide application procedure could be enhanced Including more contingencies into the procedure such as strainer pluggage, power reductions' etc. The biocide application procedure 12-EA-6090-ENV-109, Intake Tunnel Molluscicide Treatment should be revised to includethese contingencies.
: 3) Investigate the possibility of Installing a in-Situform"r sock as a means of making the tunnel walls smooth. This technology Is employed often ln the repair to sewer lines.
: 4) investigate a non-chemical means of controlling zebra mussels In the Intake tunnels via hypoxia, The tunnels could be shut for a period of time to deplete the dissolved oxygen level to the point where the mussels suffocate. The use of sodium bisulfite could bee used to hasten the oxygen depletion process and minImize the tIime period that the tunnel was removed from service.
Areas Fbund AcceptabLe
: 1) No spill eVbnts or chemical discharge exceedences 0ccrred during the application period.,
Thevendor and plant proved that the preventive blocide application could be controlled within its MDEdf permitted conditions. This is the first known zebra mussel preventive blocide application of this grand a scale performed In the U. S.
: 2) The settlement monitoring system was able to provide feedback as to whether the settlement goal was being achieved. An upgraded blo-box pumping system Was used for the first time uring this project. This design was able to perform reliably for four months as opposed to one month as inthe past.
: 3) Many lessons were learned. A betterknowledge ofour intake tunnel corrugated pipe design being conducive to zebra mussel settlement due to the eddying effect of the pipe corrugations is better understood. The demand and dilution characteristics of the lake water and Intake forebay are better understood, eof 1P 13

Objectives and Scope.
The objective of thls-self-assessment-was to assess the effecitiveness of the preventive treatment strategy using a daily or other periodic biocide application In implementing the required action specified In Step 4.7.1 Chemical Control Methods, of ENVi-8913, Zebra Mussel Monitoring and Control Program. This attribUte being:
Maintaining intake tunnelzebra mussel infestations <2iniches to minimize clumps breaking off and challenging the traveling screens. These requirements are:
a)      Requests for proposals and responSes were adequate for successful treatment.
a   Chemical feed and lab analysis.,
* Performance monitoring,
                    ,   Training and qualifications.
a   Procedure development.
Material and system compatibility.
* Compliancewith regulations.
b)      Letters requesting approval ofthe blocide that were sent tthe state requested, applications In a manner that-would achieve a successful treatment.
* Review state authorzation letter and compliance with the letter.
c),     Procedure 12-EA-6090-ENV, 109, Infake Tunnel Molluscicide.Treatment, was revised to incorporaterthe new treatment procedure and met the requirements of' ENVI-891,3.
d)-      The settlement-monitorlngsystem wasable to provide feedback as to whether the settlement goal-was being achieved. This g0al being that no more than10%
of the post-veligers measured'on the slides would exceed 500 microns.
e)       Chemical'residuals were monitored in the blo-boxes and' unit discharges. No spilleVentsW or chemical discharge exceedences-occurred during the applicaudn period. The chemical residuals specified by the Vendor were achieved inthe intake tunnel bi0-bOxes.
Attdbute evaluation was performed by:
: 1)       Review of Request for Proposals and responses from Chemical Vendors.
: 2)        Review of letters of request for biocide approval and responses from the MDEQ.
: 3)        Review of procedure 12-EA-6090-ENV-109,. Intake Tunnel Molluscicide Treatment.
: 4)       Review of settlement monitoring system and data.
: 5)       Review of chemical residual bio-box and unit discharge data.        -
: 6)       Personnel Interviews.
Page 3 of 13

Assessment Methbdology Mr. Richard Green, a peer evaluator fiom the Constellation in charge of their zebra mussel monitoring and control         Energy Nine We Point Nuclear Plant program assisted in the self-assessment.
Interviews were held withMs. Carol Grandholm, a contract and Mr. William Jung, a contract chemical applications         zebra mussel'monitoring technician, engineer for GEBetz. Reviews of Request for Proposals and chemical vendorresponses, letters of request for blocide approval and responses from the MDEQ, the application procedure,settlement data, chemical residual blo-box'and uni discharge data,                      monitoring system and Performance&Observation Program (POP) ob&sect;ervations, condition reports, Operating Experiences were performed. A site tour was given to the peer evaluator (OEs), and personnel Interviews plant systems and lay-out, and equipment used for               for him to gain familiarity with the the project. The peer evaluatoralso hadthe opportunity of observing Ms. Grandholm performing standard method zebra mussel countsron artificial substrates during his visit.
Self-Assessment Team.
Mr. Richard Green, a peer evaluator from the Constellation In charge of their zebra mussel monitoring and control          Energy Nine Mile Point Nuclear Plant program Jon Namer, a Cook Nuclear Plant Environmental Supervisor assisted in the self-assessment.
and objectives of the~self-assessment and reviewed applicableassisted In developing the Scope.
Experience events. EricMallen, a Cook Nuclear Plant                 condition reports and Operating Mussel Monitoring & Control Programowner, was responsible EnvironmentalSpeclalist and'Zebre of self-assessment team members,,developing scope                  for the overall planning, recruiting and   objectives,   scheduling, coordination, and writing the self-assessment report. All self-assessment assessment report and comments were Incorporated                 team, members eviwed the self-herein.
Assessment of Critical Attibutes.
: 1. Maintaining intake tunnel zebra mussel Infestationsto breaking off and challenging tie traveling screens           S2 Inch6s tO minlmizeclumps via  a  preventive treatment strategy using a diaily&#xa5;or other periodic blocideapplication.
a) Requests for proposals and responses were adequate for successful treatment.
Request for proposal RFP23525 was sent out to three pro-bid meeting was held on Jan. 14, 2003 and proposals   vendors for bids on December 20, 2002. A The RFP requestedvendors to provide a proposal to             were received on February 7, 2003.
furnish materials, equipent, andI management oversightto provide a non-oxidizing chemical colonization in the circulating water Intake tunnels. The       treatment to prevent zebra mussel sothat the accumulation of zebra mussels In the tunnels     treatment strategy was to be structured did not Impair plant operation. The treatment season was to run from April I thru November recommendations. The tunnels were to be treated sequentially 30UP subject to the vendors dilution water supplied by the two untreated tunnels during          as to take advantage of the the treatment      Chemical detoxification was not desired for the prpoject. Plant labor was originally envisioned perform the lab analyses; however in addition, theplantrequested            to  operate the system and the vendor to'provide this service of which we opted                     that an option be provided tfo to take.
The plant wasto perform a cumulative settlement study'during zebra mussel monitoring vendor. A goalwas set that                  the treatment season with their no more than 10%.of the post-veligers measured on the slides during the treatment season would exceed 500 microns.
Page.4 of3 13

The vendor was to work with the plant to develop a site application procedure and supply the plant with analytical procedures for determinling both process and discharge effiluent chemical residual concehtrations. The vendor was to evaluate the treatment chemical formaterials compatibilIity to ensure there would be noimpact to plant seals, gaskets, structures, and piping components. The vendor was to also determine and report Impacts if any that the chemical might have, on the Plant's Make-up Plant and the chiemicalbeing used simultaneously with continuous, chlorination of the service water systems.- In addition tomeeting the above criteria, award of the contract wasicontingent upon approval by the MDEQ to use the vendor's-chemical at the Cook Plant.
Bids were evaluated on their technical merit, the chemical's ability to be approvedby the MDEQ for use at1Ithe Cook Plant in the manner being proposed by the vendor, and cost.
The three chemicals that wereevaluated Were GEBetz Spectrus CTi 300, Ondeo-Nalco EVAC, and HOH Chemicals A-432 (Mexel)..
The A-432 (Mexel), would have required longer lead times for delivery due to its beingiproduced in France. The proposed method for delivery to the.onsitebulk tank utilized plant compressed air to pressurize the delivery tank: This methodis Unlke methods used at the piant, as the delivery trucks are equippeddWith theIr own chemical off-loading system. Static mixers were also proposed~tobe locatsd between the dilutionwatersupply header and the screenhouse connection points to the 3-Inch PVC chemical feed lines which route tothe intake cribs. This arrangement would have possibly needed additional supports, and would have taken up additionaliscreenhouse floor space. The-CT1300 and.EVAC productsare prduced inthe U.S.
and have been used successfully in the past at-Cook Plant.,
The proposed treatmentlreglmes for CT1300 and A-432 were quite compeilingdue to their.
relatively short durations. The proposed application rate for the CT1 300 was 1.5 ppm for"2 hrJday per tunnel and theA-432 was 2-2.25 ppm for 20-30minutes per dayper tunnel. The EVAC treatment regime was less desirablelat 0.25 ppm'for'4 hrs./day per tunnel. iThe:CT1300 and A-432wemr the most competitiveas far as cost was concerned. CT1 300 was selected for the project based onltechnical, cost, and MDEQdlscharge suitability, the last~of Which will be addressed later in this report.
Afllthreevendors evaluated their-products for compatibilitywith the Plant's Make;up Plant, component materialseand continuous chlorination of the service water systems. None, anticipated any problems posed by their products In the concentrations and durations being applied. None anticipated problems with the Make-up Plant provided thepre-treatrnentisystem was working as designed. A problem with the Make-up Plant R/O membranes being plugged by colloidalfmateral was had during the daily CT1300 applicatiorns. A conrsultant from Water&
Power Technologiesi Inc. hypothesized thatthle R/O element failure was due to the addition of,
,the CrT300,o which is a very surface-active cationic surfactant He thought-that CT.300 modified the negative surface charge of the col.oids In the Water and/or the negative charge characteristics of the poly-amide R/O membrane surface. This allowed the colloidal material to come out of suspenSion-and grow larger and plate out on the RIO membrane. It wasthe opinion Of the consultant that neither the vendor staff, Cook Nuclear Plant, nor himself, could have foreseen the occurrence of this situation in advance. The application procedure needed to be rpevsed to use the Lake Township Water supply during periodswhenthe CT1300 was being appl!ed.
During the first few applications, Chemistry reported that they were seeIng an increase in circulating water system demand andhavihg to.raise chlorine residuals during the'period of biocideinjection. ItUis surmisedthat the blocide was stripping off bio-mass causing an Increase- in demand during the period of blocide addition. As such, the intermittent chlorination of the.
circulating water system was scheduled and completed before the 6-hr. bioCide application each day.,
Page $of!3 5
                                                                                                     'o:1

b) Letters requesting approval of the biocide that were sent to the state requested applications in a manner that would achieve a successful treatment.
Letters of request for the two most competitive products, A-432 and CTI 300 were sent to the MDEQ for review.
A letter requesting the use ofA-432 was sent to the MDEQ, Surface Water Quality Division on May 22, 2003 (2003-690). The request was In accordance with the-vendorlsbld proposalto apply the blocide Independently to each tunnel up to a maximum concentration of 3.75 ppm for up to.30 minutes each day during the vellger spawning season to remove existing mussel colonies and to prevent further settlement. This would result In three 30-minute discharges of A-432 out each Unit's outfall (001 & 002) averaging 0.5ppm with no one sample exceeding 075 ppm. as measured at each outfall's near shore sample point. The MDEQ replied in a letter dated May 29, 2003 (2003-744), that based on the toxicity information available for A-432, a discharge concentration of 0.5 ppm will exceed the daily maximum discharge concentration of 0.021 ppm that had been established for the prodUkdC. They in turn disapproved the application under the conditions set forth in our May 22, 2003 request letter. The vendor has since run additional toxicity testing on A-432 and isengaging the services of a Michigan water quality lab versed, in the state procedures todevelop a higher discharge limit for the product.
A letter requesting the use of CT1300 was sent to the MDEQ, Surface Water Quality Division on May , 2003,(2003-596). The request was In accordance with the vendors bid proposal to apply the b6oc6de Independently to each tunnel at a concentration of 1.5 ppm.for up to 2 hours each day durnthe veliger spawning season from April thrn November. The&#xfd;reuest described that there woUld be imperfect ingdue to the preference of effluents from the North intake tunnel'to be discharged out the Unit1 Dischargetunnel (Outfall 001) and the effuents fmmthe South Intake atunnel 1 ppmto discharge be discharged   out the unit2 concentration        Dischargeattunnel as measured               ( 0utfall002)and each outfall's            approval sample point   wth awas 10:1sought mingfor
*zone.
Discussions were had with the MDEQ and Environmental management and it became apparent that the MDEQ wasdnotgoing to applh a mixing zone tothe Propsed descarge concentration2 without further demonstraon. SubsequentY, the CT1300 vendorwas able to Prsenttoxicity data to theMDEQ to support raising thedischarge lt from 0.038 ppm to 0.070 ppm. In a letter to the MDEQ on June 12, 2003 (2003-803). the Plant modified Its request to apply the biocide independently"   to each tunnel for  2 hours measured fromneachoutfall's      sample   poinper day~with    the resulitng t to exceed0.070        discharge ppm.           concentrationi The plant             as:
als statedthat letter dated June 13, 2003(2003-839), the MDEQ granted peirnisslon to discharge up to 0.070 ppm of CT1 300 from each outfall for 2 hours per tuninel per day with no changes to the mixing zone.
It is implrtant to note, and will be discussed furtherin this rmpor, that the final discharge              n concentratIonthat was granted by te MDEQ was much lower(by aafactor of 14X) thancenhat was requested bythe plant. Therefore, the possibility of a successful trewatent 6ucome Was jeopardized by theestsrctive discharge limits granted by the MDEQ. It was thought that even at
                                   'thee ws ao~sbilty o Inreaingthenfoing:ppeto 5baed'n sudie byAldn Lbs.Iwa this lowconcentration, there Would leter bateseoe p effet Jue          on the zebra mussel larvaeIn 1i 2031,00-119),theMDE grnte'pemisionto          iscarg.thatthe ulo tunnels;
                                                                                                       .07
,would   exhibit unde.eath theanadult environment    Opm orf CIhous mussels not           30 fomprtoel thatconducive would- use       eck utfll to settlement  er~dy Wth~o cangs tothernlin!
and anover them to release     effect on the slime layer time.t mPaglonfl3
                                                                                                              ~t

c) Procedure 12-EA-6090-ENV-109, Intake Tunnel MoliuscicideiTreatment, Was revised to incorporate the new treatment procedure and met the requirements of ENVI-8913.
Revision 4,of 12-EA-6090-ENV-i 09, Intake Tunnel Molluscicide Treatment Was issued on June 20,2003. The revisIon incorporated-a method to perform preventive treatments to the intake tunnels on a routine basis that are targeted at the microscopic settlement stage of the zebra; mussel. ItWas expected that applying a biocide on a daily or other routine schedule does not necessarily kill'the zebra mussel, but provides an unsuitable environmental for it to settle and colonize asystem. The scope of this-procedure revision isrconsistent with pare. 4.7.1a. of ENVI-8913 Rev. 3, Zebra Mussel Monitodng and Control Program, that states; "A preventive treatment statey. using daily or othrperiodic biocide applications Is under evaluation'. Revision 5 of 12-EA-6090-ENV-109 was Issued on supply for the Make-up Plant from July     29,2003 to provide a method for switching the water the.NESWto LakeTownshipwater         dung the period of blocide treatments so that theLNESW treatedwaterdid notenter the Make-up Plant and cause fouling of.
the RIO membranes. Both procedure revisions pefo'rmedas expected.
The Peer evaluatorcommented that In reviewing the site bicide procedure 12-EA-6090-ENV-109, IntakeTunnel Molluscicide Treatlment, that the .MDEQ limits for preventive treatmentsLwere not mentioned In the pr.oedure. We explained that the MDEQ granted permission to perform preventive treatments late In thespring of 2003. Because of this late approval,we purpseily did not state the type of biocide to. be used.or the actual discharge limit values, butreferred the user to the limits as specified in the MDEQ'S approval letter.:
TheiPeer evaluator also commentedithat more contingencies are Written Into their site biocide procedure than wererienuded in ours e.g. lossof power, loss of heat exchangers, etc.
A condition report search for the years' 2002 and 2003 was performed-on the key wordsearch,
 'Detailed Condition Description' =mussels or clams or CT1 300 or molluscicde,. The search, produced four condition reports (CRs 02159030 of 6/8102, 02290055 of 10/17/02, 03079007 of 3120/031& 03326033 of 11/22/03) related to traveling screen carryover of mussels and debris due to spray nozzles being plugged ormisaligned.. One of the screen panels on 2-OME-43,4 (CR-02159030 had broken screen mesh due possibly to corrosiodi. It Is important to note that this degraded mesh condition due to corrosIon was identified later as a failure mechanism in the fish Intrusion event:of April 2003 (CR,031 14044). All condition reports Were classifiedW'OR' (Operations Review) at the 0900 Plant Managers meetings and concluded 'thatthe WorkControl Processis adequate to resolve this issue and no furtherevaluations are needed In this CR1, New miultldiskdesign traveling screens (12-RPA-5191).made of matedalsthat are corrosion resistant
.and result lnzero carryover, have been tested and'are planned forinstallation In 2004. The 2003 preVentive .biocide treatment hadhno noticeable effect on traveling screen carryover;.
The original treatmenrtschedule of Apnrl 1 thru November:30 subject to thevendors' recommendations could not be met. This was due to the late MDEQ approval of the chemical discharge received on,6/13/03 which Impacted an earlier start, and WMO-17 needing t0'be cldosedtosupport. Intake forebaydiving operations in November during the Unit I Refueling Outage which cut off two weeks toward the end. Even If the blocide had been deemed effective, this reduced schedule window would have had little Impact, as the first zebra mussel peak spawn, of 185,500 veligers Per cubic'meter (Attachment 2) did not occur until 6/19/03anid we started the.
daily bicide treatments shorty thereafter on 6125/03. When It was confirmed by'divingj inspections of the Center-and North intake tunnels during the Unit'l Refueling Outage that the, biocide applicationswere having iittle effect, t was decided that continruing theblocide' applications would'be of little value. Insteadi we opted to concentrateour efforts into ensurng that all needed screen house diver cleaning activities of the Intake forebay were completedduring the Unit 1 Refueling Outage. Should preventiVe treatments be considered In the.future, a blocide application schedule of May 1 thru November 30". schedule should be considered similar to the schedule for service watertsystem contlnuous chlorination.
tage 7  1f 13'

d) The settlement monitoring system was able to provide feedback as to whethetr the settlement goal was being achieved. This goal being that no more than 10%
of the post-veflgers measured on the slides would exceed.600 microns.
The plant had prior experience with a sampling system that consisted of placing 8 gpmn well-,
pumps down the Intake tunnel manways and feeding extension cords and tygon tubing through the plant perimeter fence to direct the water flow to bio-boxes placed on a table on the west wall of the screenhouse. These blo-boxes would then drain to the Intakeforebay. In previous shock treatments, the blo-boxes were,seeded with live adult zebra mussels and left exposed to the treated water from the Intake tunnels during the treatment.. The efficacy of the treatment could be assessed by;counting the number of live and dead zebra mussels in the blo-boxes In-the days that followed the treatment. -WithIn two weeksI f1oowing thetreatmenti, the count was completed.
The blo-,boxand well pump'arrangement also served as a sampling system for the treated water to determine the biocide residual. This system worked quite well forthe approximate 4-weekI period it Was called upon to pump water for chemical shock treatments.
The challenge was to either find a new pumping system or upgrade the existing system to pump 24/7 for 8 months in a 6-71/sec. intake tunnel flow. Our previous experience was that the well pumps would typically give out after one month of continuous operation. This short running life was difficultto accept, as well pumps In ahome can last in excess of 20 years. After an, evaluation, an air operated diaphragm pump was tested. It would not lift the 14 f11head from the, water surface to the screenhouse grade and the idea was discarded. An Environmental Technician explained the problemwilth the well pumps to our well pump Supplier and he was able to recommend fitting out our well pumps:with aPVC sleeve and a wire reinforced tubing length with a screen at the end. This assembly'allowed the well pumpto remain submerged In the water:
that rose Into the manway, but out of the swift flow ofthe, Intake tunnel. The wire reinforced tubing and screen extended down Into the Intake tunnel flow. The flow of water rushing pas.tthe pump motor and Into the pump Inlet cooled the *motorand greatly enhanced its running life. This configuration Is similar to how awell pUmpis situated within avWell casing(..
For the settlement study,. test tube racks filled with microscope slides were placed into the blon-
,boxes to serve asartilfcialsubstrates to monitor settlement in each of the North, Center, and Sbuth Intake tunnel manway blo-boxes. For a control, microscope slides placed in test tube racks surrounded by metal cages were attached to a weighted rope anddeployed In the center of the Intake forebay west of the trash racks.
The system was set up In mid-June and operated continuously until early October when flow was observedto be diminishing on the North Intake manway blo-box. About a week later, fioWrwaS observed to.be diminishing on the South intake manway blo-box. The North and South pumps were replacedwithrnew pumps and the Intake scmens-and wire reinforced tubung.was cleaned and backflushed. From this experience it can be concluded that the pumps have a pumping.life of about 4 months before they wear out. Inthe future, well be able to anticipate this diminished performance, and schedule a pump changecut before It occurs.'
The settlement monitoring system did provide feedback as towhether the goal of no more than 10% of the settled .post-veligerswere greater than 500 mlcronswas being met. Referringto the chart (Attachment 1), with the exception of the 10/30 sample on the North intake Tunnel Manway bio-box, the average size range and individual average size Increased In all bio-boxes. Within abouta month (7123) after commencing the daily treatmentsithe South Intake Tunnel Menway, bio-box showed that 14% ofthe settled post-veligers counted Weregreater than 500 mIcrons.&#xfd; By:
the next sample date on 8/7 all of the test blo-boxes showed more than 10% of the settled post-;
Veligers greater than 500 microns. The Control slides did not show more than 10% of the settled post veligers greater than 500 microns untilo10/2. This could have been due toqthe fact that thedse slides were getting a longer duration though'!ower concentration exposure being that these slides, Page.8of13

were positioned downstream of the Intake tunnels, or that they Were suspended in'-a flow as opposed to the slides in the bio-boxeswhere the flow was virtually stagnant. At any rate, the, samplingsystem was able to determine whether the goal of no more than 10%.of the settled.
post-veligers were greater than 500 microns was being met. The sampling results~showed that the goal was not being met.
During the monitoring season, discussions were had as to whether the~blo-boxes simulated the conditions in the intake tunnels, being that the flow rate through the tunnels'was6,7 ft/sec. and the flow rate through the blo-boxes was virtually stagnant. Running the sample stream through a smallscale corrugated'pipe was discussed, however-the volume of water pumped by the sample.
pumps would have had to be much greater to simulate the 6-7 ftJsec. flow rate. This was discussed with the peer evaluator dudng the self'assessment who explained that our pipe corrugation-design creates small eddies or low flow areas on the downstream side of the corrugation which causes zebra~mussel'settlement;. This being the case, the bio-boxes do simulate the eddies orlow flow areas inthe pipe whereozebra mussel settlement occurs. itwas surmised by the evaluation team that ifour Intake tunnels were smooth, therewould be little Ifany settlement Inthe tunnels at a flow rate of:687ft.sec. This is the .casewith the Nlne Mile PoInt 2 intake concrete tunnel. The peer evaluator reported that they only-see settlement at the joint gaps where eddies occur inthe concrete tunnel.
Video diving Inspection tapes were reviewed with the Peer Evaluator from the Center and North Intake-tunnels performed inthe fall of 2003. These were ;ompared with the diving Inspection
'performed on the North Intake tunnel inthe spring of 2002. The 2003 Inspection results show that-there arettwo layersiof'3/8"zebra mussels growlng~on the,downstream side of the corrugatlonsand beginnlng to fill the Invert of the corugation, From these tapes, the Peer, Evaluator was able'to develop a theory as to how mussels Infest the intake tunnel Inthe presence of a high flow-velocity (6-7 ft./sec) through the tunnel., He stated thatmussels settle.duetothe pipe being made of corrugated.steel. Flow velocity Ismuch lower along the tunnelwalls, probably onthe order of -2'ft./sec. Eddies are created on the downstream side of,the corrugation that allows iarvai~and juvenile mussels to settle and accumulate onI the'downstream sideof the
:corrugation. These settled mussels intum move the eddy further downstream and allow mussels to settle and eventually fill Inthe entire inverted corrugation. This eddying effect could be*-
mitigated by making the tunnel walls smooth. He mention the possibility of Installing a Irn-SituformW sock as a means of maklngtheltunnel walls smooth. This isldone by introducing an epoxy sock at one end of the tunnel and allowing itto expand out to the tunnel wails and harden inplace. The result iSasmo0th'plping surface. He reported thatthis technology is employed often In the repair to sewer lines&
Twenty (20) Performance Observation Program observations (POPs)'&#xfd;were made in2002and 2003 on the zebra mussel monitodng and chemical apprications vendors. This POPs entailed observing these persons 'performing tasks on various aspects of zebra mussel monitoring and blocide treatments. No performance'deficiencies were determined from the review of the POPs.
Asit~ tour was given to the peer evaluator for him to gain familiarity with the plant systems and lay-out, and equipment-used for the project. The peer evalUator also had the opportunity of observing Ms. Grandholm performing standard methodzebraemussel counts on artificial substrates during his visit. The peer evaluator concluded that the equipment used for the project.
was consistent With Industry practices for performing zebra mussel monitoring and:control. Itwas also concluded that Ms. Grandhoim'was using the standard protocols for determining zebra mussel counts and sizes on artificial substrates.
Page:9 Of 13

e) Chemical residuals were monitored In the blio-boxes and spill events or chemical discharge exceedences occurredunit discharges. No period. The chemical residualsspecified bY the vendorwere            during the application intake tunnel bio-boxes.                                                 achieved In the Attachment 2 shows the daily chemical residual data collected boxes. This data was taken from Data Sheet I of procedure Inthe IntakeltunnelmanWay blo-Tunnel Molluscicide Treatment. The daily blodde treatments 12-EA-6090-ENV-109, intake were tunnelsfrom June 25,2003 until October 28, 2003. Daily treatmentsperformed on the Intake as the emergency service water gate WMO-17 needed to                        were stoppedafter this date diving and MOV maintenance-workduringL the Unit I Refuelingclosed to accommodatevoutage be resumed on November 19,2003 to deplete the chemical                  Outage. Daily treatments were thatremaIned In the semi-bulk container and flush the System. The tunnels were treated daily for 113 days.,
The MDEQ discharge limit of 0.070 ppm (70 ppb),Was never within the tunnels had to be kept low during thebeginning          exceeded. Chemical residuals of the season, but could be raised as circulating water system demand Increased as warmer lake blooms, and In the fall, turbulent lake conditions resulted water temperatures led to plankton in more material In-suspension., The highest chemical residual obtained during the treatment season tunnel on October 13,2003. The average chemical residual was 298 ppb In the CenterlIntake concentration for the 113 day period measured in the North Intake Tunnel Manway blo-box was Manway blo-box It was .102 ppb and for the South Intake 66 ppb, for the:Center Intake Tunnel Tunnel In theirproposal, the chemicalvendor recommended that 1.5 Manway. blo-box Itwas 64 ppb.
hours per tunnel per day. At best we Were able to deliver           ppm (1500 lppb)beo:applied for 2 hours per day, lthe Center tunnel. Therefore; thechemical      an  average   residual of 102 ppb1for 2 not achieved as measured Inthe intaketunnel blo-boxes.            residualspeclfied    by the vendor was The blocide vendor believes that flow In the tunnel atea velocity Therefore, the blocide distribution in the tunnel Is homogeneous.of 6-7 ft.isec Is turbulent by the tunnel corrugations. The low concentration of chemical The velocity profile is disturbed treatments is either not getting down in the lower dips Inthe being applied during preventive corrugations due to the eddy effect or existing mussel populations remove the available chemincalresidual exposure., The chemical. never reaches the-slime layer between             and recover from the thus mussels do not release from the tunnel walls.                     the mussels'and the tunnel wall,.
Upon his retum to Nine Mile Point Nuclear'Plant, thePeer Evaluator discussed our corrugated steelintake pipoedesign with their system engineers anddevelopedthe Regardless of pipe corstruction, the normal velocity profile                   following theory.
would   be  lower  at the tunnel wall. The pipe corrugations-magnify this effect resulting In stratification tunnel wall with the bulk flow. Any chemical reSidual in thislow    of the boundary layer of water atthed quickly consumed by the chemical demand from mussels,                  Velocity boundary layerwould be slime, andsediments residing on the.
pipe walls and not replenished by-the chemical residual experienced in the past while doing shock treatments. In the bulk water flow. This has been When we brought the chemical residual
 *up slowly In a swiftly flowing Intake tunnel it would take a long time'to overcome the chemical demand. Conversely, where welve brought the chemical chemical demand is quickly overcome, and We can easily residual concentration up quickly, the.
maintain aresidual concentration In the tunnel:
In contrast, good results can be achieved when performing temperatUres'2>68 degrees F by slowing the Intake tunnel shock treatments in water circulating water pumps In run during outage periodS. Thisflow using a stoplog or having, fewer alIows the higher concentration (4-6 ppm) bioclde better contact with the mussels residing Inthe tunnel corrugations and results In a Page,10 of 13

better kill. Decreasing flow velocity-in the intake tunnel may decrease theeddy effect at the tunnel walls and result in a chemical 'soak type* envirohment; During the self-assessment, the Peer Evaluator mentioned a non-chemical means of controlling zebramusselsIn the Intake tunnels via hypoxia. The tunnels could be shut for a period of time to deplete the.dissolved oxygen level to the point where the mussels suffocate. The useof sodium bsulrtecould be used tohasten the oxygen depletion process and minimize the time period that the tunnel was removed from service,.
A restrictive MDEQ discharge limit of&#xfd; 0.070 ppm, aslow circulating water system demand especially early In the season, and an inadequate dilution of the Intake tunnel effluent before being discharged to the lake Impacted our ability to achieve the vendors recommended residual concentrations within the-intake tunnels.
A discussion of this inadequate dilution phenomenonis worthy for purposes of planning future treatment strategles of this kind, Intake tunnel residualdata Was comparedwith'the corresponding Unit I and Unit.2 discharge data from October lr17',j 2003. Both unitswere In operation~durng th!stme period andthe clruating water system wasin Itsnormal'alignment with all threetunnels open and tunnel flow rates in'the 6-7 ftsec.'velocity range. Underperfect mixing'conditions, one would expect a 2/3-(67% reduction) dilution of the Intake tunnel effluent when it mixes with the two untreated tunnels before being-discharged to the-lake. HoWever, due to the-plant's intake forebay design, this is not the case. Because of the baffle wall configuration
'In the Intake forebay and the uneven-number of circulating water pumps (3 for U1 & 4 for U2),
effluents from.the North Intake tunnel have a preference for being discharged out the Unit I Discharge and effluents from the South Intake tunnel have a preference foirbeing discharged-out
 ,the Unit'2 Discharge tunnel. Due to the additional circulating water pump on:Unit 2, effluents      -
from the Center lntake tunnel have a tendency to be drawn toward the Unht-2 side of the Intake forebay and be discharged out the Unlt%2 Discharge. A percentreduction of the Intake tunnel residuals dueto mixing 'and system demand.wasdeterminedfor thisdata and Is presented below:-
Tunnel'Treated              %Reduction in              % Reduction In         Average Reduction In Effluent Concentration     Effluent Concentration Effluent Concentration Dischargedfrom Unit        Discharged from Un it' Discharged from Both
             .... __1__.. 1                 ...      2                      Units North                      3                          15                     9
 -Center-                     60W                        61                     61 1South                       18,                        12                     15 The bestpercent'reduction in effluent concenthationd n the plant discharges occurs when treating the Center Intake tunnel (61%). Very little reduction In effluent concentration-occurs when treating the North (9%) or the South (15%) Intake tunnels. One should be cognizant of these percent reductions in-effluent concentrations when planning future preventive treatment applications.
Summary Site Request for Proposal and Contracting procedures were used to obtain a chemical vendor to supply-chemical, equipment, and laborforthe project. The cTI300treatment was selected for the project based on technical,-cost, and MDEQ discharge suitability, Two unforeseen issues arose as a result of using-the product. A problem with the Make-up Plant's R/O membranes being plugged by colloidal material was had during the daily CT1 300 applicationsI itwas the opinionrof an Independent make-up plant consultant-that:Oneither the vendor staff, Cook Nuclear Plant, nor himself, could have foreseen the'occurtenceb ofthis situation Inadvancer,. The applica on procedure needed to be reVisedto use the Lake;Township water supply during periods when the CT1 300 was being applied. Also, during the first fewapplications, Chemistry reported that they were seeing an increase Incirculating watersystem demandand having to PageIltofI3;

raise chlorine residualsduring the period of blocide Injection. This was remedied by scheduling the daily biocide treatments after the daily intermittent chlorination treatment to the circulating water system.
Letters'of request for the two most competitive products, A-432 and CTI 300 were-sent to the MDEQ for review. The letters requested use of the products In accordance with the vendors' recommendations described In their proposals. The MDEQwould not approve discharge of the products as recommended. The plant elected to submit a request to the MDEQ and obtained approval to discharge the CTI 300 product at a much lower concentration (0.070 ppm) than specified In the vendor's proposal., It was thought thatevenat this low concentration, there would be someeffect on the zebra mussel larvae in that the tunnel would exhibit an environment not conduciveto settlement and have an effect on the slime layer underneath the adult mussels thatz would cause them to release Over time. The low concentrations applied to the intake tunnels did not have this expected effect.
Plant procedure 12-EA-6090-ENV-1 09, IntakeTunnel M01luscicideTreatment, was revised to incorporate the new treatment procedure and met the requirement of ENVI-8913. The procedure had to be revised again to provide *a method for switching the water supply for the Make-up PIant from the NESW to Lake Township Water dubing theiperiod of blocide treatments so that the NESW treated water did not enter the Make-up Plant and cause fouling of the R/O membranes.,
Both procedure revisions performed as expepted. The peer evaluator commented that more contingencies could be written into our biocide addition procedure e.g. loss of power, loss of heat' exchangers, etc.
The settlement mbnitoring system was able to provide feedback as to whether the settlement
.goalwas being achieved. This goal being that no more than 10% of the post-velgers measured on the slides would exceed 500 microns, An upgraded blo-box pumping.system was used for the "fitsttime during this project. Thls'deslgn was able to perform reliably for four months-as opposed to one month as ,in the past. The sampling results showed that the goal was not being met.
Chemical residualswere monitored in the bio-boxes and unit'dlscharges. No spill events or chemicaldischarge exceedences occurred during the application pedod., The vendor and plant proved that the preventive blocide application could be' controlledWithin its MDEQ permitted conditions. Save for spent analytical reagents, there were no waste application products to dispose of at the end of the project. The chemical residuals specified by the vendor were not achieved in the Intake tunnel blo-boxes, because of the MDEQ discharge !imits being too l0W, low circulating water system demand, and inadequate-dilutiondue to the flow characteristics In the..
intake forabay.
Strengths Nonei Areas Found Acceptable
: 1) No spill events or chemical discharge exceedences occurred during the application period; The vendor and plant proved that the preventive biodde application could be controlled within Its MDEQ permitted conditions. This Is the first known zebra mussel preventive biocide application of this grand a scale performed In the U. S.
: 2) The settlement monitoring system was able to provide feedback as to whether the settlement goal was being achieved. An upgraded blo-box purnplng system was used for the first time during this project. ThIs design was able to perform reliably for four months, as opposed to one mMonth as in.the, past..
ag     of 13' 9Ai
: 3) Many lessons were learned. Abetter knowledge of our Intake tunnel corrugated pipe design being conducive to zebra mussel settlement due tothe eddying effect of the pipe corrugations Isbetter understood'. The'demand and dilution charactedstics of the lake water and Intake forebay are better understood.
Findings None Reponmmendations
: 1) Review thls assessment with peers and vendors to develop a more effective chemical preventive treatment program,minechanical cleanIng, or revisit targeted shock treatments to the intake tunnels.I
: 2) The peer evaluator noted that the blocide application procedure could be enhanced Including more contingencies into the procedure such as strainer plugging, power reductions etc. The biocide application procedure 12-EA-6090-ENV-109, Intake Tunnel Mollusclclde Treatment should be revIsed to Include these contingencles.
: 3) Investigate the possibility of installing a In-Situform7msock as a means of making the tunnel walls smooth. This technology is employed ofthenIn thebrepair:to sewer lines.
: 4) Investigate a non-chemical means of controlling zebra musselslin the intake tunnels via hypoxia..The tunnes could be-shutfora prod of time to depletet dissov oxygen level'to the point where the mussels suffocate. The use of sodium bisulfite could be used to hasten the oxygen depletion process and minimize the time period that the tunnel was removed from service.
Page 13 of 13 \9)

Attachment'l ZEBRA MUSSEL SETTLEMENT MONITORING RESULTS 2003 PREVENTIVE TREATMENT North Intake Tunnel Manway DATES           7112003      7117/2003   7/23/2003       8/712003      8/2112003    91412003. 9/1812003  10/2/2003 10116W2003  10/30/2003 Density            15,467       74,311     108,800        782,933        249,493     958,720        >914      TNTC          (1)    130,844 Size Range (1L)   200-.330     200-460     160-830     200-1490         200-1190,. 230-3300     200-4290  230-4030    260-1600    300-1190 AvglSize (I) 248          304         352           472             357        611         941       1026        752         '522
# >500 p                  .           .0            3          '10               8        13          13         34         36           .23
.%&#xfd;500 P.                0o            0           6           20              16         26          26         68         72           46 Center Intake Tunnel Manway DATES           -711012003   7117=2003   7M230/203       81712003      812112003    914=2003   911812003  101212003 1011612003  1013012003 Density              4,000      25,244      41,600        300,267          TNTC    1,040,000        >9/4      TNTC          (1)    228,267 SizeRange (p)     200-400      160-600     160-700     ,200-1160        200-14W0   200-1550     200-3130  200-3070    230-2110    230-4290 Avg Size(p)           -242         264          301          446              400        403         550        678        674          852,
# >500   i 0          ,3            2           14                8          -4         8         19         24           28"
% >soojl                  0           '6            4          28              16           8         16         38         48            56 South Intake Tunnel Manway DATES           7!1012003    7117/2003   712312003      .81712003      8121/2003   .9/41200    9/1812003  101o 0    10116/20o3  10/3012003 Density            23,467       -79,289     98,667        509,333          TNTC      702,933        >9/4      TNTC     168,533   - 172,089 Size Range (pI)   160-360      160-600     160-830     230-1420         200-3140:   200-660     200-1190  200-1980    230-2400. 300-2145 Avg Size,(p)           244         321          362           436             499        385         398        422        557          860 0:          13
# >500 It                                          7             9              8           5          9         11         25           32
% >50o0p                  0            6         14             18             16         10          18         22         50           64 Control Forebay DATES           7/10/2003    7/17/2003   71232003.       8712003       81=2003      914r003. 911812003  1012/0    1011612003  10130f2003 Density                ND      159,467     149,333        358,400      2j383,200   1,553,600   1,888,500- 1,565,300    406,933     404,000 Size Range (p)         ND      200-460     160-400-       200-430        200.530    230-500      230-600    23D-730    330-930    300-1680 Avg Size(IL)            ND         273          288           315'            357        355         364        376        547          623
# >500 I.1              ND             0            0            0              -1         .0          1          8          17           23
% >500 IL               ND             0            0            .0              2          0          2         16          34           48 ND - No-Data TNTC - Too.
NumerousTo Count (1).- Too Mych Deus Page 1 oial

Attachment 2 2003 PreVentive Treatment Blocide Residual & Zebra Mussel WhoIle Water Monitoring Results Date    North        Center South.
CT-1300    CT-i300     CT-1300      Whole-u4,'l       wh/        yO/        'Water 5/1/2003        -                      -          75 5/8/2003  -                                       50, 5122/2003        -           ,          -        2,075 5/2912003                              -275    10 61512003                                       16..i97-5 6/19/2003       -           -1                   8-6&sect;1-500 6/25/2003            55  ' <50              60     ND 6/26/2003    .       50     <50             55 10,725
.6/27/2003            55     <50             50     ND 6/28/2003            70        70           70    ND 6/29/2003            50        50           50     ND 6/30/2003           90         80s          90     ND 711/2003            65        65           50. ND 7/212003            70        85           95     ND 7/3/2003          105         80         110 120,750 7/4/2003           ,95      110          100      ND 7/5/2003            65      <50        .ND         ND 7/6/2003,          <50     ,,50
                             -50                    ND 7/7)2003    ,<50            <50        '<50        ND 7/8/2003            80      <50             50     ND:.
7/9=2003        -           -           -          ND 7/10/2003       -                               107600 7/111/2003       "
                 -                       -          ND 7/12/2003            85       120            90     ND 7/13/2003            82         70           88     ND 714/2003.            70         80           80    ND 7/15/2003            68         87           63    ND 7/16/2003,           62         80           59    ND 7/1712003            54      '&#xfd;65 -          55'  60.500 7/1812003            67         99           69    ND 7/10/2003                                  -       ND 7/2026031        -           -          -          ND 7/21/12003       &deg;           "          -          ND 7/22&2003:       -           -                     ND 7/23/2003        -           -331750 7/24=2003            72      1286         -.72     ND 7/25/2003        -                                 ND, 712612003        .. _      -          -          ND 7/2712003        -           ".         -          ND 7128/2003.       -           -          -          ND 60         99           56    ND 7/29/2003 7/30/2003,           52      131            86     ND Page Iof4

Attachment 2 7/31/2003            771       52     571331.000 811/2003            65        81     65     ND 8/212003            53        98     55     ND 813/2003            54&#xfd;       868    52     ND 814/2003          <50         76   <50      ND 8/5/2003          <50         70   <50      ND 8/6/2003            53        73     56     ND 8/7/2003          <50         89     55 18050 818/2003            51,       89   <50      ND 8/9/2003            :56       76   <50      ND 8/10/2003            53        94     56     ND 8/11/2003            51        89   <50      ND 8/12/2003          <50         67   <50      ND 8/13/2003            61        83     58     ND 8/14/2003            60        90:  <50 450000 8/15/2003r            57       74     .50    ND 8/16/2003             58     127      62     ND
  /817/2003           82, .72         89     ND 8/18/2003             76     119      74     ND 8119/2003             74       84     80     ND 8/20/2003             68       88     56     ND 8/21/2003             76     139"     68      926,400 8/22/2003             81      113     84     ND 8423/2003       . 76,    167      54     ND 8/24/2003             64       95     72     ND-8/25/2003             64       92  . 80     'ND 8V2612003            60      102. 56     ND
:8/27/2003-           67     163    . 66     ND 8/28/2003          . 87     169      62    88100 8129/2003     .      57      182      57     ND 8/30/2003             931    180      175    ND 8/31/2003             72      159      91    ND 911/2003             75      110     67     ND 9/2/2003           <50        86   <50      ND 913/2003             88      138  "7        ND 9/4/2003           <50        60     511   .i3,950.
9/5/2003,          <50       118     63     ND 9/6/2003             55   . 60   <50      NDO 9/7/2003             556      5       53    ND 9/8/2003             55     . 75      53    ND 9/9/2003           <50       132    <50,    ND 9/10/2003            60     <50       63   5,925 9/11/2003         .80         102      81 , .ND 9/12/2003   .         56       80      55    ND 9/13/2003            57      110      75     ND
                    <50                50 k60     N.ND 9/1!4/2003.
9/15/2003           <50        60,   <50     ND V91I12003             51       89      55    ND Page2 of 4

Attadhment 2 911712003     58Its 149        531I  ND 9/18/2003. 79     125       59   37,650 9/19/2003     70     134       70    ND 9/2012003     72. 120       61    ND 912112003     50     128     <50. ND 9/22/2003    100     108     <50     ND 9/23/2003     50     103,      55    ND 9/2412003     95     105       75    ND 9/25/2003    <50     134     <50    21,025 9/26/2003     52      90       52    ND 9/27/2003     55:    105     <50     ND 9/28/2003    <50     130       54    ND S9/29/2003 .<50     <50      <50     ND 9/30/2003M   <50      70       52    ND
   .1011/2003    82      73       67    ND 1012/2003    <50    <50      <50       ,
10/312003     58      98       63    ND 1014/2003:    50    204      <50     ND 10/512003,   <50     110       57    ND 10/6/2003     75      70     100     ND 10/7/2003     75,     72     100     ND 10/8/2003     8631    89       50   21,625 10/9/2003     65    265       '91    ND 10/10/20031   <50     112     <50     ND 10/11/2003'    67    '193       72    ND 10/12/2003     50    1921     <50     ND 10/13/2003     69    298        64    ND 10/14/2003     51    117      <506    ND,
.10/1 5/2003    <50 , 93        <50     ND 10/16/2003     62      89       71   36,425 10/17/2003     50     130       57    ND 10118/2003    <60    130     '<50     ND, 10/19/2003   -       -      -          ND 10/20/2003     <50   <50       <50     ND 10/21/2003    <50    <50      .<50     ND 10/2 2J20 03  <50     141      <50     ND 10/23/2003
 -              ND      96       82     ND 10/24/2003     ND     166       75'    NDI 10/25/2003     104   166       161     NDI 10/26/2003    209    183       131     ND 10/2712003     190      73      83     ND 10/28/2003      94      89      90     ND Page 3.of 4

Attachment 2
_      North Center South Max.       209    299   16i Avg         6    102    64 Min.       <*50         <So 5so 0  _
No Treatment ND No Data Page 4 of 4

Attachment 3 Self Assessment Plan Assessment Number. SA-2003-REA-0030QH AsSbssment Topic: Zebra Mussel Monitoring and Control Program 1.Scope of Assessment.
Evaluate the effectiveness of the preventive treatment s-tategy using a daily or other periodic blocide application in implementing the required action specified In Step 4.7.1 Chemical Control Methods, of ENVI-S913, Zebra Mussel Monitoing andContro Program. This action being maintainingintake tunnel infestations k 2 inches tominimize dumps breaking offend chalenging the traveling screens and systems downsMtrearr. ENVI-8913,:Zebra Mussel.
Monitoring and Control Program satisfies one of the objectives of NRC Generic Letter 89413, thatbeing Action 1- Flow Blockage andBlofoufing MonitoringlConttol.
: 2. Expectations ofthe Assessment.
* A'review of contracting activities, obtaining an MDEQ discharge permit, procedure revision,
:equipment mobilizatIon and Operation, and chemical residual and settlement results monitbring, will reveal any program weaknesses in the goal to maintaln intake tunnel infestations <2 Inches to minimize clumps breaking pff and challenging the traveling screens and systems downstream.
: 3. Critical Attributes:
The'Planfintake tunnels were'treated dally with awbiocide to control zebra growth in the Intaketunnels to<02 inches.
         -a)'      Requests for proposals and responses were adequate for successful treatment
* Chemicalfeoed and l6b analysis.
* Performance monitoring.,
* Tralning and qualifications.
* Procedure development
* Material and system compatibility.
* Compliance wlthlregulations.
b)Y      Letters. requ&#xfd;ting approval of the biocide that wiere sent to the state requested applications in a manner that would achieve a successful VeatmenL
* Review state authorization letter and compliancewith the letter.,
c)       Procedure 12-EA-6090-ENE-1 09, Intake Tunnel Molluscicide Treatment' was revised to incorporate the new treatment!procedure and met the requirements of ENVI-8913*.
d)       The settlement monitoring system was able to provide feedback as.to whether the settlement goal was beintg achieved. This goal b:eng that no more than 10%
of the pcst-veligers-measured'on the slides would'exceed 500 microns.
e).      Chemical residuals were monitoredIn the bi0-boxes andunit discharges. No spil events or chemical discharge exceedences occurred during the application period. The chemical residuals specid bythe vendor were achieved in-the Intake tunnel.bc-boxes.
                                                                                          ,Page :1of 2"

Attachnment, 3:
Attribute evaluation will be performed by:
: 1)      Review of Request for Proposal and responses from Chemical Vendors.
: 2)      Revlew ofletters of request for blocide approval and responses from the MDEQ.
: 3)       Review-of 'procedure 12-EA-6090-ENV-109, Intake Tunnel Molluscicide Treatment.
: 4)      Review of settlement monitoring system and data.,
5),     Review of chemical residual bb16-box and unit discharge data.
: 6)      Personnel Interviews.
: 4. OrganIzatlons to be NotWfied:
: 1. Environmental and contractors
: 5. Assessment Schedule:
Start              12/10/2003 Completion:       01/30/2004 Milestones-12/3/03 - Arrange for a peer evaluator 12/5/03 - Collect data and send out familiarization packet topeer evaluator-12/10-.Peer Evaluator Arrives from New York. Introductions, Site ToUr, Review of Data 12/11 - Interview wlthCarol Grandholm.
12/1 1-Interview with. William Jung, .Complete data collection.
1116/04 :- Draft Assessment Report Complete..
W/30104 - Final Assessment Report Complete.
6.Assessment Checklist:
: 1. Perform introduction swith peer evaluator and familiarization With Cook Nuclear Plant.
: 2. Tour of screenhouse and vicinlty for understanding of equipment placemient and sampling activities.
: 3. Review of Request for Proposal and responses frtomchemical vendors.
: 4. Review of letters of request for biocide approval and responses from the MDEQ.
: 5. Review of procedure 12-EA-6090-ENV-109,'lntake Tunnel Molluscicide Treatment.
: 6. Review of settlement monitoring data..
: 7. Review of blo-box and unit discharge chemical residual data.
: 8. interview with Chemical Vendor - Willliam Jung
: 9. Interview with Settlement Monitoring Technician -Carol Grandholm, 10! Discuss concluding remarks with Peer Evaluator.
LeadAssessor: Eric Mallen Team Assessor: Jon Hamer Peer Evaluator: Richard Green, NineMile Point Nuclear Station Reviewed By            ~             / /~r           pprovedByI                                  3 Lead'Assessorl Date                           Re     nsible Management/Date Page 2 of 2

APPENDIX 9
           ,1

Cof TECHNICAL A NALYSIS REPORT Indiana Michigan Power Company Donald
'               C. Cook Nuclear Plant One Cook Placei Bridgman, MI 49106 Juldy.28,2003 Walter & Power T~e-d'no~gi es,fur, ob# 757 EArth Tech Contract NumlberA-1'94844 3740 West 1987 Southi Sat LAC City, Utah 84104 Phone: (1) 974-5500 vkix (801) M97393 WW~wpt~coni
            \_

0:a1818 U U Z TECHNICAL ANALYSIS REPORT July-28,2003 INTRODUCTION This repont summarizes the findings and recommendations from the conslting servces.
conducted by Water &Power Technologie Inc. (WPT) for Indiana Michigan Power Company(AEP), Donald C. Cook Nuclear Plant in.Brkdgman, Michiga Blair:Zordell of Indiana Michigan Power Company. ordinated the consuting. seces.
wPT and Nonnan Norvele would likJeto thankJay Adamis John.Caron Jr., Jonathan Cross, Jon Hlze, Eric Mallen, Tom.i Summers, Jeff Weam, and Blair Zordeil for their time and efforts duringthis consulting service.
BACKGROUND Indiana Michigan Power Company (AEP) owns and operates Donald C., Cook Nuclear Plant, an electcic            facility in Bridgman, Michigan. The water at this facility is suppliedfromLake Michigan. Water teatmentplantuedwateri                s LakMichigan wateuthat i'sprovided hum the plantsno-n-e'ssen'tial service water. Supple0ental revemi osmosis (P.)    system feedwater can bepurchased frm theLake Township water supply.
Th water fteaftnt plant provides high'pufrfy mke-up water for the steamgeneration plant and .oterplt     needsTh watau*          mtpl*t isof a standard designusmng pzireiatment a 2-stage RO system~ and a three-bd,=   deicu       iersstemi (cation exchange, anion exchanger, and mixedbed) with a acm degasifier. Overall, the 1"system               mn RO system has provided eliable serviceand opermions.
 . rm       trg       ions and operaiona.l conditions..
necessitateda diffen*t water treatment prgrm orzebra mussel courbL A biMocide fibm GE.1Betz (SpearsC 1300)was chosenfor an ealuation'thatcomni&#xfd;c on June25,2003- Within one week theRD sygstem&#xfd; 14 soag fbed rsm nraed over 100%/.'The MDelements were, chemically cleaned andthey retutned tooigienal performance. After refturing to service thcyimmeately fiulyed again w'ahintwwo-weeks following ead*fio              of thi biocide*,
the RO elements failed due to high diflenhl pese.Also, salt rqection decreased.
A decrease fin saltrejcto is a-Jfailure of the me Mbrn    torjqmtepasg ofsalt i,ons.
This isoibsered and measured by an increase inrm            c,            which is usually an increas in permeate dlissolvdsolids.
The      e        t w      plac     One"a fth*eROede     mw        it,toAvista Technologies, Incf*.r a membrane autopsy. GE Betz pafmed micrombological analysis ofthe*ater. Anoui consultant and&co              ig seMrvie (Water & Power Water & Pow Technologies. Inc.                                                  Page 2 of IS
:'                  3

03188002 Technologies, mc) was retned to assit wiM a recommendation. The pupose of ths consulting service was to address the following concerns:
I. Find probable cause ofhigh differential presstue in reverse osmosis (1O):system.
: 2. Find probably cause ofdcreased salt rejection (uinc         permeate conductivity).
: 3. Provide recommendations to resolve high differential pressures.
: 4. Provide recommendations to prevent future decrease membrane salt rejection.
INFORMATION"& DATA The following inbOrmation and data were used to write this report
: 1. On-site plant meein& discusion of water system operation, review of data, and
       'walk down of system on July 15, 2003 with operatlor% eineers, supervisors, and other technical stafftodiscuss operational problems and R)Osystem failure.
: 2. Follow-up m" fting and exitmei         repot on uly 16,2003.
: 3. Betz        brn MawtAl Safety Data SheekEffecive Date 27-of-1988.s
: 4. Ondeo Nalco EVACMolHusk Contl Tieatm Confidential Product Profile.
: 5. Cook Nuclear Plat Poced ur Numbe 12-OHP-4021-062-01 Rev.3A,

==Title:==
ReversemOsmosis Operations.
: 6. Cook Nuclear Plant ProcedueNumberN          12-OIP-4021-062-012 Rev. la, TYide:
Revere Osmosis Membrane laing.
7 Change of Procs Notification for NPDES Perit No. M10005827 dated October 28,1996 Meining to two modifications of RO unit and dry lay-up ofboiler.
S. Process printout of data and charts ofRO System for past three weeks.
: 9. Make-Up Plant Flow for Cook Nuclear Plant 1-0-1 Chmicas-               # B0903.
10o Lake Michigan Water Analysis Summay and plaatotak water analysis.
: 11. Cook Nuclear Plnt, Infrmation PM -2291-           -      Rv. Data Sheet 1.
Troubleshooting Control For Plant, (Pages 25-30).
: 12. GE Betz Microbiological Aalyi LabotoyI: 83416, Repoted 14-JRL-2003, Cook Nudemr PlantAEP CORP.
: 13. Avista Membn Au"o             Repo AE3P at Cook Nwh Plant, July 2003
: 14. Betz letter ofJanuary 4,1994from W.K.             ek to R oMos,          wvoith alttchment, petining to wam-Tio CT-2 and CT-4 products.
15.&#xfd;Emal mm .C. Malln tofWroam J               an w-lbur e c          e     g di mIea-tisudft addition to rteCtinank
: 16. Printed            o tiled, "Bjncde Teatment,          i     Coriati      Make -
UpPln Opcrzting PlaALt.&#xfd; 17,HawlG..]T'dC                     *      .y    OFA            9M - VanNostrand Reinhold Company. 1977.
is. WhitGo.Cliflbrd. THandlxokofC!kinationandAUfk aive' D.sit ecftwzI      Ee Van Nostrand Renold Company. i992..
19.]Kim,Pulsig Ymng-Hn.1995.'
C ua~    ntawndF&=a dm- heoyaqPra=6. TallOas
: 20. AWWA. 'O=peinalCotnhuof            Coagulaionwrd~iratinPwcesse(AWWA M. mIM37). t'Ed          American Water Works Association. 2000.
Water & Power Technologie       Inc.                                        Page,3 of8 I

21L Byrne&#xfd; Wes. Rewverse Osmoisis -A.Prawical Guide.For ndusfial Users 2 E4 K Tall Oaks Publishing Inc- 2002, 22.,Fditec TecdlumcaMwm* The Dow Chemical. Cbman.                 1995.
WATER TREATMENT PROCESS I -
Reactio iTar*
en~nuiLi Mukkeda Frain Syte I
                                            *-Ie)        I            2sets.of3
                                                              -1 *beds' OBSERVATIONS &,FMIINGIS
    .1. The. existin Rd systan was deIsigned and biiltyby oics, Incorpone&
: 2. The R0 systemcbnsistsftwo sepa*eparal-eski -EmEchrytm is a two-stage design consisting of09.pr    e vessels in thc firststage an46,pressr vessels in the second stage. There ar 6 elements per r essurevessel
: 3. The RO elmets areDowFilmtec membran(                 BW30-365), wch are high.
surface ame brackih Water RO delenkes.
4.T*i RO.pereate flow ranges from 250-305 gpm and RO reject
: 5. l                r er w all RO5=              e tu Lake biclU a And Hrqur a permit.
: 6. The RO system                  process has been succefd Waed for many yea with few*!*blms
'Water & Pwer Technologies.. Inc                                                Page 4 of 18 5 -.

0,318,88002
: 7. Nonnal make-up for the water treatment plant is raw water from Lake Michigan with an alternative supply available fiumthe lake township municipal supply.
: 8. The LakeMichigan raw water quality is very good with an average temperature of22C (720F). The water has a positive LSI and could frmncalcium carbonate scale without pHadjustment with acid or scale inhibitors, 9.. The RO system feedwater p1H is-adjusted with sulfiuic acid to a pH of 5.7- 6A to prevent'scaling and overallidepositionon the RO element membranes.
: 10. GE Betz peformed-a microbiological analysis of the ;aw lake water (July 14h).
and found that overall the water contained only low lemvlsof biOts.
J1.. A new biocide program for zebra mussel control was s*a                          Iedon June25
: 12. The biocide used was GE Betz Sctus CT1300 (alkydimethyl benzyl a           n chloride). This is also known as an ADBAC qua.
13'. About 70 ppb cfactibiocide is injected for about 6 hours per day. It takes about Ior  5-30 minutes               the biodderto dear the forebay.
: 4. Within several days after the addition ofthe e~wwbiocide there was!a rapid increase. in* dstage sit denitmide,            (sl):feed pregum,    diffe*
SDIwasg.atrthan  n *i*re    sinthe RO a fedwat.
desst stae and in
: 15. The SD lfor                 e       raw l      w is ty pcally 1'A , andfor iz meae municipal water 3-3 4. With'the addition ofthe biocide the SDI'was >5.
: 16. ITe biocide appeared to.rquire about 4-6 days to migrt thugh the water treatmnent system and produce, high pressures inthe RD system 1i'stae.o
: 17. RO system failure resuled fiom hydraulic ptun and failure ofthe elements, producedfrombhigh 'fedand                            idifferentipresrs l        .pressureresul rom inoganc prilesfouingi the ILO elements.. Excessive pressure resulted in elements telescoping,. being compressed, and outer fiberglass shells splitftig 18, Avista.Technologies peribrmed a membrame aut                              :.The primay goal oftbe autopsy was to determnine whether-Spectnus CT130D fuldW the memibrane. In their                        ons they concluded that Cr 1300*fuls F'dmte* BW3O0 membranes and that a diflrn materia beevahiated.
: 19. Th autopsy fouant deposit f-om the membrane consisted of dy possiblysome aluminum hydroxide, baceria, and bacterialslime.
: 20. The autopsy revnaed at 10-poundl gain in the clement weight dire to particle foligThs s onidre'mden~t olng u ol result in very high-pxsuredropa pu gggdue tothe very                                         oft p            .
: 21. The 5.0 bag filters,at changed weeky.                                 .
  .;Z Thse 1.0 ~&cartridge filters= iarecnged eivery' thre ees
: 23. iron levelsini 'the lake make&#xfd;-up waiter recicfup toA097 mgIL and an orange, precip~itate has beentnoticed on the 1.0~ ciartridge filters.
: 24. ThieRO elements were replaced about 2 y&#xfd;ears. ago.
: 25. The RO eements are&#xfd;dmicaly ded'about every 2 yeams...
26.. The RO elemenits wer dremically, cleaned afte .rthe first fouiling and the elements; to 115e1tooginial 1pera0FFIting CORditionprior Wthfoing.t
: 27. The water treatmn system haisno on-line pro-ess particle or turbidity anayzers.
      .Opertional and Maintenancedata art recorded, logged, abd can be trended.
However. a software prpgram, suchas 'FTNORM", to normalize nd-trendi Water & Power Technologies, Inc.,                                                                  Page 5 of 8 CR.

0        8 80 02 membrane-operat* data isnot used. Normalization software could not be found totrend water atithe cooler Lake Michigan-watertnemperature, which is 220 C..
: 29. The atopsy also revealed a positive Fujiwaa test that is indicative for membranes damaged by chlorine oxidation. Avista recomended that plan dechlorination procedtrs-should bereviewed.
: 30. A decrease in sat.rejection(imcrmse in conductivity) appeared within the same time fiame as the fouling.. Note - 0,cal* corine (*di*'"O?
: 31. Chlorine is injecein frontof screens at the fretbay for 90 minutes daily, Monday through Friday, for slimew   cot. Also, chlorine is used to disinfect other variolusplantsystem& Chlorinuisadded as partof*       tphemmsystgem
: 32. Chlorine is remoed prior to te RO system with granmular activated charcoal, beds.
31.The granular activated charcoal (GAC) beds are changedout (rplaced) yearly and are due for replacemet
: 34. Lake Township water supply is a supplemental supply to the plantiand adds only chlorine and alum to their municipal water teannent pMocess-35; The Lue Township municipal Wt sup*l was used during the past 3o days&
: 36. The plant treated water is chlorinated to about 0.5 mglL as free available chlorine (FAC) and the Lake Township,muncipal ater range'sfrom 2- 3mnWL FAC.
: 37. Lake Township water issadded afer the GAC beds andis not dechlorinated.
: 38. The water treatmntsystem hias no on-line prcess 'chlorine analyzms or oxidation-reduction potential (ORP) analyzers to detect chlorine resi&duasrbefor the RO)system elements.
DISCUSSION.
The main objective ofths rportis to fid teprobable caus of high dffcmrndda pressure infthe O system and prvide a                         to resolve theproblem. Other bj      es include finding the probable cause of decreased salt rejection and provide recommendations0t resolve the problem. Also, provide rec m datis to prire these two reoccurrencs and increase RO eement lonevity.'
Based on the, fA ondaa identified Ab               and other fidfmation, the -causeof the element faiflure was due.to high differentia pressure. Th high differential Pressure res&led fiom mostly inorganic colloidal fouling and sequevnial plugging offthe WC) membrane. bThepubable              ofthe coMMi fingwas.thediinofa newbiocide, (GE Bel zSpectrus Cr .1300).into the waterteamn program fbrizbra mussel1 control..
Thbe use of the decreased salt rejection was'duetlopoxdation by,chlorination. This may have been qused by a hilure ofthe carbon filer (GAC) beds to remove the clrine. The following will be divided into -individu    b*ut relat   topics to stpport this vonclusion&#xfd;..
Mollusk Control and.Quats Zebra,mussel control is difficult and the options are few.         J1a-naietiiin n treatment co=sts eliminate many options 0)ep=&dng,,on the restrictions and:
cces             the mostviable chemical ta            i ons am chlorinatiog chlorine dixdquaternay ammonum compounds(quats),and other~mateials combined withi Water & Power Technologies, Inc,                                                   Page 6of 18i C,

0 31880 0 2 quats, such as ONDEO Nalco's EVAC mollusk control treatment that is a combination of endothall id and dimethyl alkylamine.
CookNceawPlntprevious              ind cUorindi        for zebra musscontol. After carefl evaluation f                      options, the change was made in June to a new program provided by GE,Betz (Spectrus CT 1300) Spenais CT 1300 isau                   a ammonium compound called akyldime-tylbenzylammonium chloride (ADRAC).
Ouaternauv Ammonium Compounds fausts)
This general family of cationic wettin agentsjis soetimes rferd&#xfd;       to as cationic surjfctor cationic deterents. They are also called quaternary ammonium salts, qate*nary amie compounds, quats, and. QACs. Quats are atypeof organic nitrogen compouindin which the molecua stfructreincludes a centalnitrogen atom joined to four origanc groups-as wellastoan acid radical Peaavaletnitrogen ring copounds are also considered quaternazy aummmium compounds. They ae A cationic surfmce-active compounds and' tend to ]be adsorbed oto,sunfces . Theire are hundreds oficationic; detergents. dassfid as quats. They have the following uses: dtelagenk. dinfctank-cleanse, fungicide, etcp. Not all quats are chemical!y the same or perform the sam, Alkvldimethvbzlammoninm choride (ADBAQ.
ADBAC is an abbreviatinand genel name for atypeof quat. The                 are many typesof quaternry detaigents called ADBAC. Al are includedin the geea dassification as an ADBAC, but each compound is a lttle differept An example ofa diffrent t               Ofu that is notan ADBAC is bmnzalonium chloride. Spectnus CT 1300 is an ADBAC quat Smvface Adsorpgon of ADRAC Aquote from aBeta report states, -ADBAC has a simng affinity formany kinds of uspended solids and subsrumes ich e anionically (negavly)chrd? A series of laboraty and field studies conducted by Rohmband Haas Company eva.ated the degree, andratethatthe ADBAC Quatis le               a      y bound to suspended mt and other subsrates. Radioactive labeled Quat soltonat c e                 o of 0.01 ppm and ppim were ue Or studies to detennine adsorptive caateitc with dfifferent types of matials. Thuge studies appeared to be conducted with-natura surface wvater. MAO 400W ppm turbidity, a~ndl30ppm alum concentration the ADBAC was 100% adsorbed in 30 minutes. Howver, this is a consiiderable amount of surface-active adsorptive m ateria the quat to be-adsorbed 'onto. The'av~eage L~ake Michigan,turidity I's les thanop andonly 5 ppm alum is added to the water treatment sys.nlThis may not be enough sus 1pendedsolids (sil and coWlid) snd alum to rmnovethDMBAC quat T pial, ADBAC and other quats ame removed from waeer by their adsorption onto. day,particles.,
,i'lmingTendeny of ADBAC and Other Oaats Quats act in-A manner Vpry'shInilar to ar filming amn.They form a monomolecla film on almosta srfaces (concrete metal filter media, 'Cand RO                   -m bnes). Some qu afeus.d a filming (bari&#xfd;r)=crsion idhibis. Mast colloids and 'day havea, av     uface cha         U.T cationic. charge and filming tend      y ofthe-quats wvud alow D       rmovalby adsorption oto c          particles. Due to this fihmand adsorption Water & Power Tedmologm, Iuc.                                                     Page 7oflS A3

0' 1,8 81 0 012 tendency:and the low level of ADBAC administrated (70ppb), several dayswould probably be required for the ADBAC quat tomigrate tbhough the piping, multimedia filtem, and GAC beds to reach.the.RO element membranes and affecttthem.
Sicaling and Fouling of RO Membranes Deposition Of depositsin RO elements is the result of scalingand/or fouling. Scaling occurs when'the'solubility limit ofa salt is exceeded and the salt crystal precipitates-near the surface ofthe element membrane. High feed pressures are produced when a_
sufficientramount of scale is deposite& Depending on the type of scalethat has the potential tozbe depositedscaling can usually be cntoedby adjustmentofthe pwith an.acd, chemical scale in*ibitor, or decreasing RO recover rates (reject               on)
Fouling is more complex* Them are two general types of fouling: biofouling and particle u.iooung Be                msltsfaro the gowthof living bacteria and/or fungi on the membrane.      ice       lg   isw       he material deposits on the membran* b             not grow on the membrane. Paclescan be from living (or once iiving) and limliving materials. L!vig(or onm iving) *plidsare the bateria,: Ilmg, algae,,protma or t     dead components. Nonliving particles are inorgaoic minerals and organic materials; Partide Srzes hnoganic particles can becassified-based-on their izes., These sizes areas follows
* Sand:          50 microns to 2 millimeters (visible to the.uman -eye)
Silt:          5 - 50 microns (the'largest ofthese may be visible)
* Clay.            1- 5 microns (not visible to the un-aidedeye)
     -Colloid:;         Less than 1 micron         are too sall for a light mic       pe)
Sand,&#xfd;sift; andc*ly are partiles that will settle. Coloidsare very smai),,f y divided' solids!(that do not dissolve),that remain dispersed in water due to their small size and.
electrical charge. -Most of the colloidal patile in natural surface water haea negative electricalcharge andtend torepel each othe. This repxusion mp nts the parti*les from%
clumping together, becoming heavier, and setltligout. -A well-designed and operated psystem               can typicay remoe particles 1.mictrnand greater.
Colloidal fouling ofteVere osmosielementsj s-acommn problenL It.         lcan sriousl impair performanice by.lowiing producd-tiviy aind somietime decreasing' sat eect~ion An early sign of colloidal fouling is often.anineadprsueifrntlamsth sysem.Colois iclue mneal lay, isoubl inrgaicminerals colloidal silica on,corrosion products, and water U.eatinent chemicals such as alhmn, frric s*il. or catioic polyelectrolytes (polymers).,
The tam cay can have twom                 I*can re. to day as a particle sine dassifibation or day.as a mina             inorganicsilt and colloidi that     istay s          innat rface watr mineral cays. Clayis a rock term and like mmot k it is made up of a number ofdiffe        min         varying proportions. They am a family of hydrous aluminum silicates. Clays may also contain magnesiumn, 'nun potassainu        sodium, and are Water & Power TechnologiesI.                                                     Page Sof 18 qI

usually mixed with other minerals. Also, they have the ability to adsorb many different materials. Mineral clay could be used tordreove phosphate from water, but would not be as efficient as alum and line. Therefbo X-Ray surface analysis (EDX XRD, etc.) of a membrane or SDI filter showing aluminum, silica, oxygenandlOr any of the above-mentioned minerals could just be colloidal day particles and nothingdse.
Ouantification of Particles Early quantification ofpartides was performed by total suspended solids (TSS) analysis.
A known volume of water was filtered through a 12.micron filter, and then the-filter was dried and weighted. The need for lower levels of measrement.and in rmI-tie resulted in turbidity measurement becoming popular. Turbidity is an inirect measurement of pa ticles by passing Ig h            a waterisolution andmeasurn how uch             t is reflected. by the particles in the iquid. Turbidity  ismeasured  in Ne*qpheometjC   Turbidity Units (NTQs). The lowetheNTU,      W       efewer partiesin thewat. Dueto rotcen advances in technology, particle    coutem     sing ers and computs can now measue the exactnsize and n          of pariclesina liquid. The higher the TSrtrbidity value ofth wa, theWgea th mberofpMaicles and ins te.hbig thefoungp na The best available tedology fo determining fouling potential of reve             smosis feed water is the wiue ntof the Silt Density index-(SDI). This is sometimes refenred to as the Fouling Index (F).AnSD)Iis det*rined by the initial time it take to filter water tho au0.45- (micron)membanm.fierat 30 PSIand filla .5,00macoainer. Afilr the waer isallowed to flow to drain for-15 minutes a second 500 nl container isfilled and timed. These two.ited valuesarc used ina fbrmula to calculate thelSDI. Awell-operated m         pal drinking water treatment systm, using surface water asthe soume water, should be able toD remove most paiticles greawtertan'0.5 micronik produce a water quality of 0.-0.2fUs, andaSD1of 3,-4. T feWaMr to,an ROcement should have a <55DZandan SDIof <3'spre&br Rartide (Conoii) Removal Thie best multimedia filters can only remove particles down to about 10 microns.
Cartridge filters can elcffcively rmne particdes'down to I micron, but this is only cost effective with nominal filtration, One-mironremoval with absol.u9tfilfftion isusualy too expensive Typical, in a conventionalwater treatmentpad             particles smaller than 1o micrns rremoved by coapation, foccatio, s me                      on, and then fltmution.
Coagulation: is the chrn,`n together of vainy fine partices clod)it lre atce (floc)"cauised by.the use 'ofchemicals (coauns) Coagulants ar"usually catoni.,
eidnics, such as alum, feriC choride" and synthetic organic cationic poly-lectrolytes (poymr). *The coagulants partially n             ae             electrical chrg of fine particles allowing them to come closer tVOehe and to fism large clumps. This' clumping together makes a easier to sqarae. solids from the water by settling and Mateing. The gatherningtoieher of the fine partidles after coagulamtiontof~rm larger particles by a press'ofgentlmixing is called,.fc            La Th sedimentation and fltration of the floc is how the very fine paticles (coIloids) areremoved from the water Water & Power Tecinologies, Inc.                                                  Page 9 of .8 1,9

03S188s0 02 Usually, the greater the,number of colloids present, the better the coagulation and flocculaion p~rocess.
Theprocess used in a conventionaltwater plant is called conventional i1tmtion. This process usesr a clarifier (sedimentation basin). The water btreat plant at-Cook does.
not have a carifier and this process is called,direct filtration. The sedimentation step is omitted and is notrequired due to the'water quality of the lake. In conventional filtration a largewfln particle is developed and removed by sedimentation. With direct filtration the ce islnot allowed to grow as large and is removed with themedia (usuallysnd).
Also, in direct filtration, particles ame removed by sticklng to media that.has a cationic (positive) charge given to the media by the coagulanti The particle attraction to the&#xfd; charged me~dia isiweak and the particles arxe mnoved by backtwashing.
Hypothesis for Why the RO Elements Fouled The behavior of colloidal particles is of fmlcm. importancie in water treatnmen processes, especially for reverse osmosis systems The R() system rMUMnoMve A particles greater than1 micron sothat the only particles "mainihg we colloidal, i Sia. The autIopsyr*veald the f*doantpositconstedof very fine clays and other colloidal materials n w fouling wasprincipally inornic-in naure and was:a resul Of
 .colloidal claydeposition. The intectioncbtwe'ooidal paiclcs in supensionad
:other*meiasurfhces depends on many variables:
a Water chemistry.
       - Surface chemistry and charge of particles.
         *Sturfacechemisry nd chargeof media srfaces.
         *Kinetics, ofib particles,5&#xfd;the wate~r, MAnd surfaes each interact with,
.Most of these variables are intaated. Options foraltaringthese variables canincud, bp am not limited to: addition andior adjustment oflcoaguiantsl        pH polymers, othe polyclectrolytes, oxidants, mbiing conditions, And. biologcal activity.
Coalias and natiOnie MaMAteIaS Colloids tend to cqfry. a negative charg on their carte surfce. ]BY havin thisngaiv common charge they win tend to repel each other.. They resist.coming into lose                        4:
proximity with each other and do not, combine to form large partcles. Withths-cag neutralized orwnoved,-fo example: w ith the addition of a cationic p olyme, the &#xfd;clloids are mor hlely to coajgula into lar particesandfllouttofsoltion Tischarecan, be neutrali*d or frucied by,positivly ca*d (cationi) materials such as alu*mium femic coagult;cationic poIyclectmlyt*e*(polymers) and other cationic materials.
These positively.charge materials att         lmselvesto               de RO Plvarmide WPA Membranes and Cationic Materials Most RD membrane elements used today rene           plyamide (PA) thin-film membranes. This
-isthe RO membraneelement that is usedlat C*oAkNuc               Plant. PA mebranesare a thin layer of aromatic polyamide extruded'ont a less dense polysulb'nMsubstrat, The.
PA thin-ftil membrane most commonly used-in water puwifcati.onintentionally has a ne.gative surfie charge characteristic. The negative char         of the colloids andthed             A Water kPower.Technologies, Inc.                                                     Page 10 of 1S ii,

0 31 8 0.0 2 negative charge of the membrane surfice repel eachotherand this helps prevent colloidal foling. Only chemicals that are compatible with thispnegative charge should be allowed to comeinto contact with a PA thin-film me brane. For ammple, only anionic (negative.
charged) suritctas should be used to clean an anionic (negative chaged) PA membrae. ,Cafinic.(positive charged) suifictants should not be used" Condusion Based on the above information, I hypothesize that the RO element ailum was due to the addition of the.GE Betz Spectrus CT 1300 (ADBAC quat), which isa very surfac-active cationi surfactant I think that the SpectsCT 1300moded the negative surface charge of the colloi.ds in the water and/or the negative charge characteristics ofthe PA membranesurface. This allowed the colloids to'come outof suspensioand grow larger.
Additionally, I believe this mateW couldalso affect the surface charge of the media in the mutimcedi sand filter and decremparticulate removaL Alsq, I believe the GAC beds were affected and chloprne removal effidency mayhave been.reduced Alsc, since it was time to replace the GAC beds, thebeds may have been exhausted and unable to removethe choine, A is the opion ofthis consultant tha neither the stafrofGE Bet Cook Nuclear Plant, nor myself could have tbreseethe oocwrewe of this situation in advance This hypothesis could probably be roven* exprimentally by measiing the overall chaw -                  ofthe water versus the addition of.the biocide Themeasurement However, I recommend using a streaming current detecto (SCD) instead of a ZP; meter because a more accurate and repeatable esr ofcharfge ca be accomplished.
Salt Rejection Salt rection is thep         t ofdissolve* salts (ions)that re* x*td (removed) bythe
-ROmembrane An 6nwease in permeate codctvy usuly idcates a decrease in salt rejection r laking04-ings. DCM=esed salt rejectionoccurs When theRO membrane isr dm ed by chemicalattack. G                .enerafl, three diff.rent conditions canproduce chemica attack on polyamde(A           mbne E xe ding operating and cleaning limi Organic solvents ame iprobable in this situation andvwill not be discussed-Onmfing and Qeamift Limits The Fidmtec membrane (8" BW30-365) is an aeelent membrane and a good choice for this.application. The pH rne ontous   h               aon is 24 f and the maxmu operatingitemperature, it 113F. These have not been xceeded fbr normal opeation.
Water &Power Technologies, Inc.                                                Page 11:of is I'-

03188,002 arsh and frequept chemical.cleaning will shorten membrane life, typically by incieased.
saltp         while mild and seldom cleaning will-extend the membranelife. For regular cleaning the preimue pH for acid cleaning is nolower than 2.0 and the            ed pH for alkaine (caustic) cleaning is no higher than 12.0. Both of these are at 300C (860V). For extended element life, it is best.not to oexed this tempeatur A 6-hour soak is usually adequate. The pHnge for short-tem cleaning (30 min chemical contact) of this membrane is 1.- 12. Adjust and maintain the pH during cleani 'possiblef Always, acid clean first and then follow with an alkaline cleaning. Acid deai;gremoves inorganic.salts and caustic cleaning removes inorganic colloids (sit) silica, biofihms, and orgnics. Please refer to the FUmtec Technicalmanualt for moreinformation.
Oxidation Chemical attackon PA membranmes usually occsfromoxidation by chlori              . At present, I feel that Filmtec has themost chlorine resistant PA,Umemb es.&#xfd; The Dow; Fimtec Membranes Product inmnrmaton sheet has the operating limit for the free ailabe clhlorine (FAC) toleance: of the BW30 membrane as <0.1 ppm, but in reality the chlorine toleane is more important The I010ing is a quote from Dow Tech Facts,"When Filmtec membranes (PA) are used in the reverse.osmosis process*, the RO eed must lb6edechlorinated to pwet:
oxidation otfthe mmbra         Fdlntec membranei have some chlorine tolera nc before noticeable ossof salt ejection is observed Evental degradation nmy ocau' afiu app mate 200-,o1000 housofexposure to 1m*iL offrdeeblorine (FAC). Therate of a          depends on various feedwater characteristic Under anline OH conditions. chloie a kis                    atea      or.acidic p. An acidicpH is
-prefrrdfor a bettbiocidaleffect duingchorWnatio ChIrineattackisalsofasterat:
higher       imp ueand at higher
* ofheavymetals (e-g. imo), which catalyze membrane degradation. Ifdechlorinationupsasoccur ina Filmteq RO system, and ifcariected'in a.timely manner, membrane damage can be minimized."
This means that the PA membnm has 200- 10W ppm-h toleranCM of free available ch&#xfd; i FAC). Ifthebacuiaer                  is ony 2O0 ppm-h tk          membr*a*could operate 2W hours at 1 ppm.FACG(.3 days), or 2000 bou.nat 0.-ppm FAC (83.3 daysj) or 20,000 hours at 0.01 ppm (2.28 yea&#xfd;r)i          h wds, lyamide RO membrancs are essentially zeocblin m            toleknt The pe           of any free hblqfoe wiff result in some damag to tie rembrane. Howev;,this da                 might not be noti6eableif not,,sev=e.
Chlorine Tolerance *nd'nLA Please note.,that at*be eait meetingl was'in error about chlorne exposure at low pH being more aggressive than chlorine-exwposr at high p11 Accord~ig to Fimftec, chlorine atter is leiss severe at neutral or acidic PH1 than alkaline p1 When chlorine is &#xfd;addedwt wateir, hypochlor=usacid (Hb)iintalyfre.Dpending on pff and temperature hypchkuusacid separates into ante opnn nwater which is thehypoclIlorite ion (6wr). HypochlNowusoai a almost twice the oxhdaton power and more than 10.
fimesthe disnfectm ability of-hypochlorite ion. However, hypochiorous acd is a weak acid and because of incomplte disassociation is poorlyionized. Hypodclorite ion, which water & Power TeclMoogies',Incmae1                                              Page!2 of, f111

03188002 formisat a higher pK-is more completely dissociated and ionized. This must W the reason that the hypochlorite ion is more damaging to the RO membrane than the hypochiorous*acid. At a pH of 6.0 and 20&deg;C, about 95% ofthe fiee available chlorine~is as hypochiorousacid and about 5%is as hypochorite ion.
Dechlorination Free available chlorin, also known as free chorine, is best removed fromw         by fihtein     ough grmramar activated carbon (GAP andlor ig'ecf`ng chemical reducing aguts. TheGAC system must be properly designed for the amount oftClorine to be removed. The-GACis consumed and exhauksed in the tmoval ofeclorine., Also, GAC can be a growth media for biological'activity. ThereRfr GAC should be replaced on a regular basis. Depedingon service conditions, GAC beds are usually replaced every 6 to 12 months.
Chemical reducingg agents that can be,used to remove chlorinewae sodium-metabisulfie, sodiumbisuMte, sodiumisulfit, sodium tiiosufa* andc mlfia dioxide. Sodium metabitulfite (SMBS) is themost common agent used and most cost effective The SMBS should be of food grade quality or better, fiee of impurities, and not contain a-ivtm (catalysts) such as cobalt Som.eimes SMBSisscobalt-actva               o shorten reaction time with chlorineand oxygen. Cobalt and iron can catalyze and enhance the effects ofchlorine oxidation on PA membranes. Aso, do not use sodium thiosulfate because this material dependin on water chemistry, can fo~rm colloidal sulfu.
Intheory, L-34 mg of sodium nmetabisui&e will remove:1.0 mg/L FAC. Bu, in actual practice, 3.0mg of SMBS.ii normally used to remove 1.0 mg/L ofFAC. Th'eactual amount .required.can be better detrmined with a good on-lie process analyzer for free:
chlorine. Solid SMBS.has a shelfl of 4-6.months under cool dry stora condition&.
However, in a day tank the sluioncan oxidize when .eposedto air. The following is a typical solution rife in a day Pik t.N Solution Lire
                            ....       0        2-.3 days 20         1month.
10L30     6lmonft-Itnmay be more costqetietieto purchase this material as a'30% aqrueousliquid than as a dry solid. Permeate or deionizedwater should be used for dilution water if thesMBS solution is to be made from the dry matera Monitoig              :.                                          '
In the past, the existVminwae treatment system mnoorouring has been adequafte Howemer due to the,recent developm~ents, monitoring for particles and chliorine, should be considered. Monitoring can.b perOrmed by grab samples (pointii tim) 4r process inst          ion(real-ime). Monitoring for particlesis presey p           mWithSDI analysis. 1do not believe that turbidity monitoring is necessary. H.owev,      ifthe decision Water      Powe" Tecinologies, Inc.                                            Page 13 of is

03 1818002 is-made to add turbidity monitoring I feelthat the best turbidity and particle monitofing equipmept is manufactured and sold by the HACH Compaiy.
SDI Analysis SD! analysis-is the best indicator of RO fouling. However, this test is-time cnmin and suffers froAmiacrcy and precision (repeatability). I do, not believe an on-lin~e process unit is ncpessaryo perorm this test and that a reguar grab-sample would be-adequate. Opeitions can determine the fiequency that SDI analysis is required. The pudIase*ofa portableSople SDIanalyzerfrom SDI Solutions can increase.Adcuracy andprision ofthe* SDI analysis and save valuableopatortime. lhave personally used these unft and can.reicmend teL Their phone numbe is 972422-1212' and-website is www.,simplesdi.com. They sell for about $,
Chlorine Monitoring, Due to the poor free Chlorine toeranwe of PA membranes, the low level presence or
 -absenceof chlorine should be,monitored with on-linei    -id    netto istrumentation can activate an alarm to alert opea'ators and shut down the RO system when frchlorine isdetcted. There are three e a type ohlorinean                      : ORP, Coloknimetic, and Amprometric.
Oxiatin-Reduction Potential (OMP analyzes do not measure fmu chlorine. directly.
They measure whether the water is under an oxidative or reductive environment. A disadva g ofORP is that the readings may be affected by other components-in the water.. For exmple. the reading is affected~by water pH and combined'chlorine.. ORP analyzers'are not specfic, but due to,the low.co~st, owmfnce and simplicity, this ania    e has been commonly used in place; dfc       rine analyzers in indusaprocesse.
ColoriMetric analyzers are primarily used'by therdrink[ing Water indsty. :Colorimetric analyz*e onsist of apphotodectric cell and a light sourtwdetects a variation in color prducedina sample. steam with-the addition ofa reage specific for chlorine. This analyzer uses consumable reagents that mst be refilled each month. Whilethese&#xfd; analyzers&#xfd;are very eedbeand'acculratthey ar, mad tI deetWlrnei h                          g of 0.5 to 5 mgfL. T&#xfd; low     levdeof detection is not elia6te eno6ugh fo tiappliction.
Amperomearic ainalyzes have gained populait during thfast severa year*du to new innovatioms    lpicialy they eonsist oftwo le*tids that arm mersed in a continuous water supply., The electrodeswae made offtwodissimilar metals that measurea cangein-urrent-flow bwe n that is diecly r"               uondl to the =munt ofdarine sidual, Iin hewater. They can be used to measure free or comnbined chlorine. ThIereare many variation& For this application fee that thbest chlorine analyzer Woud be the Hach 9184. Polymetonmakes.this and Hach                  now nst            . At aofpU <7.5, this unit hasa detection l       of10ppb*rOCand20ppb.feeh repeatability s 5 pbfor HOCI and -10 W6pbtfrice chlorine.- After the pH is adjuste for your RO system, almost all free available chlorine in the feed water wou** exst as HOCL Thfis means that he detection limit would. be 10ppb.. Due to an ion selective-membrane, the Hach 9184 measures o          re chlorine and has almost no intediuincet fiom Water &Power TOchnologies, Inc,                                                 Page 14 of 18

0 3 1 t80.02 combinedchorines or other materials The reonse time          <90 seconds. Immial Iainna    nce isrequired for this unit The bc     entcostofthis analyzer is $3,400.00 fEnersenc, Procedure In an RO system, fouling usually occurs in the lead element of the Id stage. Ifhigh, pressures develop in the I' stage, consider removing the lead element in each vessel of the i* stage, pushing the other elements forward in Oach vessel and then i&#xfd;stalling a clean clement as the tail.element (6A in each vesseL This is good for emergencies.
Wate &Power Technologies, Inc.,                                              Page 15. of714

O3      818 002 RECOMMENDATIONS
: 1. Do not allow ADBAC quat bioad            into the make-upwatergoing into the water tratmentplantor the RO System feedwater. Use an alteraive source&#xfd; such as.
the'Lake Township municipal wat when feefin the biocide tothe intakes
: 2. LakeTwsi municipal water must be dechlorinated beforeetrngteR system. A sodium metabisulfte (SMBS) injection system -isrecoipmended., The SMBS shoulddhe injected upsrem of the retention/blend tank Adequate m                g should be etnsred with a staic mxeror adequate downstream pipelengths after, injction. Add onl enough SMBS toxrtmove tc&#xfd;&#xfd;hand no exess SUBS.
: 3. Replace the granular activated charcoal (GAC) in theg       carbon fitersat least one a year. Change out more often if Jsot adequft.
: 4. Free available chlorine houldbe lessthan 0,020      Mg/L (20Mppb)fr allwater entingte Ri se              The greatthe expo e of the RO elements tochine-the shortr the life of the elemean
: 5. Installa fiee advilable chlorine (FA) analyzer before tfe RO:system. The analym uld have an aarm toalt the oprato and ,shut down. ithe RO system should the FAC exceed.20 ppb.
6A iat         and main*an a trending pop        for Net Permeate Flow,(NPF):and noraliedsaltcontent such as Dow Filmtec's        MFORM .Thisfis a-flee rga that is used to nomal        memb me                   data ToTefctively evaluate systmuperformance, it is       cessaiy to-compare permeate flow and salt passage data at the same conditions. Ask DOW for help and assistance with'this program Their program was developed'for their membranes and they wiprod6-te"hnica
:assistance ifyouaret persisten Talkttothe            du hat sold you the Fil*.tec RO elemens This is a rivsupport ervcett they wilQ.prde
: 7. Stop,and cbeically dean the RO                   whenevt s The nbormalizedprte          flow draps by 160/0,"
6 tlie    normaiedlsalt cont*t ofthpomeate waternc*         amsesiby 10%
* TU diffrellntial pressure, (feed pressuecncentratepressure) increases by, 15%K from the refterene conditions (initial'perfibnance established durin the first 24-48 hours of oprto)
  ,8., Continueto adjust the RO feedwater pH to 5.7-61.1However, consideration should be gven to lower the p11 even more to a range of 5.5-5.8. Below a.p1R of 6.0 the aluminum ion;s are solu~billized and'cannot produce any precipitates.
: 9. TheADBAC qua biocide may have coated the graiu                 media inthe multimedia filters Consi~der cleaningthe fil~ters withigh pH water (II) and bleach (5,ppm FAQ with,a 6-hour soak. Then back.was and flush the filters           .svenrdatimeLs.
Water & Power Technologes,,Inc.
Wll. ,rO~l.Tg~o~l*,*                                 Page
* 16&#xfd;of 18; i       /7O K

0 3 88409 APPENDIX (Exit Meeting Report of Wednesday July 16,2003)
Date:, Julr 16, 2003 Location: Cook Nudear Plant, Bridgmrn, ML Fromn: Norman IL Norvele, Water& Power Techndogies (Earth Tech)

==Subject:==
Exit Meeting Report (Wednesday 1:00 pm - RO System Failure.Analysis)
Problem Definioun
: 1. Th plaun ROsystem is eperiencing high dffeni pressmn min the firststag. Th system failed due to high diffrnti p         w.sand the esulting rupture ofthe RO elements.
: 2. TIhRO system .is also expez6iencng a-decrease in salt rejection (an incream   ,in condudiv).
crent Expectations
: 1. Find caus of hi~gh diffrenial(A delta) pressure and high SDIs.
: 2. Provide r-ommendations to resolve.
: 3. Find cause ofde*esedzsalt rejecWio
: 4. Providercomme tons to re Summary ofdobswations and infomation Gathered
  . Operation and performance offtlRO sysm was good.until the addition.ofbiocide.
: 2. Afer new bioide was added high SDIs (>5) were fou&
3   After new biocide was added high.Als were producedlin th firs stages.
: 4. After new blocide was added element failum ozured..
: 5. After new biocde was added salt reection decreased-
: 6. The only changein operational and chemical parameters was theaddition ofbiocide.
Hypothesis of Failure        ..
There are two,major control metod for mollusks, chlorine and Qualernay ammonia compounds (Quats).:Quats are also called qateary amino compounds and QACs. TIh term Qu is ageneral name and rvf*ln to over 100 compounds., Quat are cationiC.
suractnts(4terent).They    ae xcellent. cleaners and biocides., They areused in many household prodcts such as 409 Cleaner, Lysodisintn 'and Odor Ban-,
Indusrill they arc-usdas eanera, disinlfetats boioies,; iamcorrosion inhbitor, GE Bez CIam-TI (CT 1300) isa Quat biodie."
Theat i amanner very similar to a filmng amine.. They fofm a mono'molecularIlm (coating) on l surfaces (cocrte, meta% filter media. ganular activated dharoa, and' RO membiknes). Quats can               removed from water by beingadso)e on cay particles-Water & Power Technologies, Inc.                                               Page 17 of 19
:.                                             *I '

03188002 Most colloids, such as clay particles, have a negative charge. RO elementmembranes also have a negative char. Theseneaive chargeS repel each other and prevent colloids from sticking to the vmebran smface and produghih                        p        .
Quats arestrong cationic muflctants. I think that the Quat biocide (CT 1300),has coated the colloids and membranes and allowed the colloids to stick on the xsuitce of the mmbrane. Thi respuled in a high diffrential pressure. Also, I believe this has changed the nega               on the colloids and allowed them to stick together, much like a cationic coaglant polymer. A# ony, I think that the Quat has also coated or filmed the zraiula activated dOarcoal (GAC) bed and that the GAC bed is no longer effective for dhlorine removal. The available chiline could possibly be oiizing and, damaging the membrane.
Low-level analysis of Quats may be difficuilt to detect because-Quatdecomposes rapidly inthe eirone           is adsoedonclay, and iadsorbed on thewalls of sample container. There is also a reaction between alum and Quat Recommendations
: 1. Do no allow any. fith Quat (CT 1300) to enter the RO                       andsstem
: 2. Replace the GAC media immediately. Rent GAC skids if necessary.
Consider cleaning the MMF with,high pH water,(I) and bleach (5 ppm FAC). Then badkwash and flush with dean wate.
4.. When usingCT 300,. obtai make-up water from other inlets or soues such as city water.
5.: If detentim time and' nwralizmn time is inadequate for the higher chlorination
*levels             in city water, consider renting or,using additionalII GAC bedsonly for city water or injecting sodium metabsufit (SMBS).
: 6. Consider jadig additional, p essmonitorn equippment:sui as cdoueanalyzer and particle or tubidity monitos ..
: 7. You shouldconsidet the addition of sodiummetabisufittbe ohelp reduce chlorine residuals bero~re the RO elements ,prelfably before the retention tank in order to have adequate contact time between tho chlorine residual'and sulfite.
Nor"anit.Nrvenle, Ms.
Water& Power Technologies, Inc. A division ofEarth Tech Water& Power TednolOgII In:.                                                   Page 18sof 1}}