Text

Tennessee Valley Authority - Sequoyah Nuclear Plant - NPDES Permit No. TN0026450 - Discharge Monitoring Report for May 2013
	ML131680573
Person / Time
Site:	Sequoyah
Issue date:	06/12/2013
From:	John Carlin; Tennessee Valley Authority
To:	Morgan C; Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation
References
	TN0026450
	Download: ML131680573 (121)
	v • d • e

W, Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000 June 12, 2013 Ms. Christina Morgan Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6 th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243

Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) - NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FOR MAY 2013 Enclosed is the May 2013 Discharge Monitoring Report for Sequoyah Nuclear Plant. There were no exceedances during the monitoring period. If you have any questions or need additional information, please contact Brad Love by email at bmlove@tva.gov or by phone at (423) 843-6714.

I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significantpenalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

\Sit"V e President Sequoyah Nuclear Plant Enclosures cc (Enclosures):

Chattanooga Environmental Field Office U.S. Nuclear Regulatory Commission Division of Water Pollution Control Attn: Document Control Desk State Office Building, Suite 550 Washington, DC 20555 540 McCallie Avenue Chattanooga, Tennessee 37402-2013

PERMIT-EE NAME/ADDRESS (Include Facility NamelLocation if DifferentJ NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)

(SUBR 01) OMB No. 2040-0004 Address P.O: BOX 2000

- -JjNTEROFFICE OPS-5N-SQfN) TN0026450 1101 G F - FINAL SODDY - DAISY. TN._3373__84. .. PERMIT NUMBERI DISCHARGE NUMBER1 DIFFUSER DISCHARGE Facilyjj _MA - SEQUOYAH NUCLEAR PLAN.T Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATTN: Brad Love From 1 13 105 101 1 To 113 105 1 31 SYEARi MO DAY I ToYEAR I MO I DAY NO DISCHARGE E_

NOTE: Read instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE **36.4 04 0 31/31 RCORDR CENTIGRADE MEASUREMENT 00010 1 0 PERMIT Req. Mon. DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS TEMPERATURE, WATER DEG. SAMPLE ... 24.2 04 0 31/ 31 MODELD CENTIGRADE MEASUREMENT 00010 Z 0 PERMIT **** 30.5 DEG. C. CONTI CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX NUOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 2 04 0 31/ 31 CALCTD UPSTRM DEG.C MEASUREMENT 00016 1 S PERMIT 3 DEG. C. CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX NUOUS FLOW, IN CONDUIT OR THRU SAMPLE 1732 03 0 31/31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. MGD CONTI RCORDR EFFLUENT GROSS REQUIREMENT DAILY MAX NUOUS CHLORINE, TOTAL RESIDUAL SAMPLE

0.014 0.026 19 0 24/31 GRAB MEASUREMENT 50060 1 0 PERMIT 0.1 0.1 MG/L FIVE PER CALCTD EFFLUENT GROSS REQUIREMENT MO AVG DAILY MAX WEEK TEMPERATURE - C, RATE OF SAMPLE 0 62

0 31131 CALCTD CHANGE MEASUREMENT 82234 1 0 PERMIT 2 DEG CONTI CALCTD EFFLUENT GROSS REQUIREMENT DAILY MX CIHR NUOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the t e Vice President 423 843-7001 13 06 12 information, the information submitted is, to the best of my knowledge and belief, true, accurate, 4 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, U OF PRINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations. ER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED ___________________________CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Veliger monitoring data is included as an attachment. The following injections occurred: 1. Floguard MS6236 (max. calc. conc. was 0.064mg/L-limit 0.2mg/L) 2. Biodetergent 73551 (max. calc. conc.

was 0.039mg/L-limit 2.Omg/L) 3. Spectrus CT1 300 (max. caic. conc. was 0.038mg/L-limit 0.050mg/L)

EPA Form 3320-1 (REV 3199) Previous editions may be used Page I of 1

Mean# of Water NOTES:,%

Sample Date Mean # of %Settlers Water Sample Date Asiatic Ter LOCATION Gravid COLLECTED BY ZMIm3 Temp. ('C) Clams/m3 Temp. (C) Asiatic 01/04/2013 0 0 8 01/04/2013 8 1-ISV-24-1234 WAW 01/09/2013 0 0 29.7 01/09/2013 29.7 1-25-545 CR 01/15/2013 0 0 30.2 01/15/2013 30.2 1-25-545 PPG 01/22/2013 0 0 24.9 01/22/2013 24.9 1-25-545 BB 01/2912013 0 0 8.1 01/29/2013 8.1 1-ISV-24-1234 BB 02/05/2013 29 100 30.4 02i05/2013 30.4 1-25-545 PPG 02/12/2013 0 0 28.9 02/12/2013 28.9 1-25-545 PPG 02/19/2013 0 0 27 02/19/2013 27 1-25-545 PPG 02/26/2013 0 0 8 02/26/2013 8 1-ISV-24-1234 JAG 03/08/2013 0 0 28 03/08/2013 28 1-25-545 PPG 03/13/2013 0 0 9.02 03/13/2013 9.02 1-ISV-24-1234 ACL 03/18/2013 0 0 28 03/18/2013 28 .1-25-545 PPG 03/26/2013 0 0 28.3 03/26/2013 28.3 1-25-545 SRR 04/02/2013 0 0 25.9 04/02/2013 25.9 1-ISV-24-1234 SRR 04/09/2013 0 0 33 04/09/2013 33 1-25-545 PPG 04/16/2013 0 0 33 04/16/2013 33 1-25-545 PPG 04/23/2013 13 50 15 04/23/2013 15 1-ISV-24-1234 PPG 04/30/2013 0 0 33 04/30/2013 33 1-25-545 PPG May 2013 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDESJ MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000

- INTEROFFICE OPS-5N-SQN TN0026450 ] 101 T F-FINAL SODDY- DAISY TN 37384 PERMIT NUMBER , DISCHARGE NUMBER BIOMONITORING FOR OUTFALL 101 Facility , TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY

]

IA tl DTIK or-olrf I I1 EFFLUENT IYEAR &3MO Fro OSIDAY 0J T IYEAR 3AY MO IDA NO DISCHARGE ATTN: Brad Love NOTE: Read instructions before completinq this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE, r ANALYSIS r MAXIMUM UNITS AVERAGE MINIMUM AVERAGE MAXIMUM UNITS IC25 STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS CERIODAPHN IA MEASUREMENT 23 TRP3B 1 0 PERMIT .******** 43.2 ******** PERCENT SEMI COMPOS EFFLUENT GFtoss REQUIREMENT MINIMUM ANNUAL IC25STATRE 7DAY CHR SAMPLE >100.0 23 0 1/180 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 PERMIT 43.2 **** PERCENT SEMI COMPOS EFFLUENT GFtOSS REQUIREMENT MIMINUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified person John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or /

persons who manage the system, or those persons directly responsible for gathering the s ent information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 06 12 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SI "RINCIPAL EXECUTIVE I including the possibility of fine and imprisonment for knowing violations.I R OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED I CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

Toxicity testing was conducted May 12-17. A minor deviation occurred during aliquot preparation for the sample collected May 17. The TVA Senior Toxicologist and Quality Assurance reviewed the deviation and determined that WET test results remain valid. Further discussion is included in the Quality Assurance section of the Toxicity report on page 13 of 113.

EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of 1

PERMIT-EE NAME/ADDRESS (Include FacilityNamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMRJ (SUBR 01) OMB No. 2040-01004 Address P.O. BOX 2000

... -(I NTEROFFICE OPS-5N-SQN). TN0245 103 G F - FINAL SODDY - DAISY,[N 37384 PERMIT NUMBER DISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND Faciliy TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT ATTN: Brad Love YEAR From l13 1 05 1 01 1 To 1 13 1 05 1 31 MO DAY I YEAR I Mo DAY NO DISCHARGE El]

NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE MEASUREMENT 7 8 12 0 16/31 GRAB 00400 1 0 PERMIT ****a*** ** 6 9 SU THREE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM MAXIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE 8 8 19 0 2 / 31 GRAB MEASUREMENT 00530 1 0 PERMIT ** 30 100 MGOL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH OIL AND GREASE SAMPLE 2 2 19 0 2 /31 GRAB MEASUREMENT 00556 1 0 PERMIT 15 is* 20 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 1.155 1.263 03 0 31 / 31 RCORDR TREATMENT PLANT MEASUREMENT 50050 1 0 PERMIT Req. Mon. Req. Mon MGD SEE RCORDR EFFLUENT .GROSS REQUIREMENT MO AG DAILY MX PERMIT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my / TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the dent information, the information submitted is. to the best of my knowledge and belief, true, accurate. 423 843-7001 13 06 12 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SI,, lfffOF PI CIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. F..FICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED _CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

EPA Form 3320-1 (REV 3199) Previouseditions may be used Page I of 1

PERMITTEE NAMEIADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000 S-IJNTEROFFICE OPS-5N-SQN) TN002645 I 110 G F - FINAL SODDY - DAISY TN 37384 PERMIT NUMBER DISCHARGE NUMBER RECYCLED COOLING WATER FLacj _ TA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MONITORING PERIOD EFFLUENT Froml YEAR 10Sl 01 TDl i0S , 31NO ... DISCHARGE ATTN: Brad Love NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS TEMPERATURE, WATER DEG. SAMPLE MEASUREMENT 04 CENTIGRADE 00010 1 0 PERMIT REPORT DEG C CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS TEMPERATURE, WATER DEG. SAMPLE 04 CENTIGRADE MEASUREMENT 04 00010 Z 0 PERMIT 30.5 DEG C CONTIN CALCTD INSTREAM MONITORING REQUIREMENT DAILY MX UOUS TEMP. DIFF. BETWEEN SAMP. & SAMPLE 04 UPSTRM DEG.C MEASUREMENT 00016 1 0 PERMIT 5 DEGC CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS FLOW, IN CONDUIT OR THRU SAMPLE 03 TREATMENT PLANT MEASUREMENT 03 50050 1 0 PERMIT Req. Mon. MGD CONTIN RCORDR EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS CHLORINE, TOTAL RESIDUAL SAMPLE MEASUREMENT 19 50060 1 0 PERMIT 0.1 0.1 MG/L Five per CALCTD EFFLUENT GROSS VALUE REQUIREMENT MO AVG DAILY MX Week TEMPERATURE - C, RATE OF SAMPLE -

MEASUREMENT 04 CHANGE 82234 1 0 PERMIT 2 DEG C ******** CONTIN CALCTD EFFLUENT GROSS VALUE REQUIREMENT DAILY MX UOUS SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the information submitted is, to the best of my knowledge and belief, true, accurate, - - 423 843-7001 13 06 12 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, SI T F'PRI KP6PAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 33204 (REV 3199) Previous editions may be used Page 1 of I

PERMIIT-EE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)

(SUBR 01) OMB No. 2040-0004 Address P.O. BOX 2000

... -_(INTEROFFICE OPS-5N-SQN), TNO026450I 11 F- FINAL SODDY'- DAISY. TN 37384 PERMIT NUMBER DISCHARGE NUUMBEýR RECYCLED COOLING WATER Facility_ TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY - MONITORING ERIOD EFFLUENT FYEAR7 MO I DAY YEAR I MO DAY NODISCHARGE ATTN: Brad Love From 13 1 05 1 01 To 13 05 31 NOTE: Read instructions before completing this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE

CERIODAPHNIA MEASUREMENT 23 TRP3B 1 0 0 PERMIT 43.2 ****** PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL IC25 STATRE 7DAY CHR SAMPLE MEASUREMENT 23 PIMEPHALES TRP6C 1 0 0 PERMIT 43.2

PERCENT SEMI COMPOS EFFLUENT GROSS VALUE REQUIREMENT MINIMUM ANNUAL SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAMErrITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of l*w that this document and all attachments were prepared under my TELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified personnel John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the P nt information, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 06 12 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, T' .CIPAL EXECUTIVE including the possibility of fine and imprisonment for knowing violations. CE R AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)

No Discharge this Period EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of I

PERMITIEE NAME/ADDRESS (Include Facility Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved.

DISCHARGE MONITORING REPORT (DMR)

Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) 0MB No. 2040-0004 Address P.O. BOX 2000 J[NTEROFFICE OPS-5N-SQN) i TN0026450 118G F-FINAL SODDY - DAISY, TN 37384 PERMIT NUMBER LDISCHARGE NUMBERJ WASTEWATER & STORM WATER Facili_ TVA - SEQUOYAH NUCLEAR PLANT Location HAMILTON COUNTY MAnITrRIiirz PFr-l:ln I EFFLUENT FromIIYEAR I M130 1-3TI051I01 I... Toll Y" 1105131 MO I DAY 1 NO DISCHARGE j] -*

ATTN: Brad Love NOTE: Read instructions before comoletino this form.

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS OXYGEN, DISSOLVED (DO) SAMPLE MEASUREMENT 19 00300 1 0 PERMIT 2 MGIL TWICE/ GRAB EFFLUENT GROSS REQUIREMENT MINIMUM WEEK SOLIDS, TOTAL SUSPENDED SAMPLE **

MEASUREMENT 19 00530 1 0 PERMIT

100 MG0L TWICE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX WEEK SOLIDS, SETTLEABLE SAMPLE 25 MEASUREMENT 00545 1 0 PERMIT MJL Ill ONCE/ GRAB EFFLUENT GROSS REQUIREMENT DAILY MX MONTH FLOW, IN CONDUIT OR THRU SAMPLE 03 MEASUREMENT 03 TREATMENT PLANT 50050 1 0 PERMIT Req. Mon. Req. Mon. MGD **ONCE/ ESTIMA EFFLUENT GROSS REQUIREMENT MO AVG DAILY MX BATCH SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT PERMIT REQUIREMENT NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document end all attachments were prepared under mYnTELEPHONE DATE direction or supervision in accordance with a system designed to assure that qualified person I ._.

John T. Carlin properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the ' i infonmation, the information submitted is, to the best of my knowledge and belief, true, accurate, 423 843-7001 13 06 12 Site Vice President and complete. I am aware that there are significant penalties for submitting false information, '6L6 &E"OFVNCIPAL EXECUTIVE TYPED O including the possibility of fine and imprisonment for knowing violations. O--

T ER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY TYPED OR PRINTED "_CODE COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments here)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.

EPA Form 3320-1 (REV 3199) Previouseditions may be used Page 1 of 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE

SUMMARY

Report Date: June 11, 2013

1. Facility / Discharger: Sequoyah Nuclear Plant / TVA

2. County / State: Hamilton / Tennessee

3. NPDES Permit #: TN0026450

4. Type of Facility: Nuclear-Fueled Electric Generating Plant

5. Design Flow (MGD): 1,579

6. Receiving Stream: Tennessee River (TRM 483.6)

7. 1Q10: 3,491

8. Outfall Tested: 10_1

9. Dates Sampled: May 12- 17, 2013

10. Average Flow on Days Sampled (MGD): 1760.46, 1548.33, 1552.94

11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: May 14 - 21,2013

13. Test Type: Short-term Chronic Definitive

14. Test Species: Fathead Minnows (Pimephalespromelas)

Daphnids (Ceriodaphniadubia)

15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101:10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC2 5 = 43.2%

17. Test Results: Outfall 101: Pimephalespromelas: ICQ5 > 100%

Ceriodaphniadubia: IC_*_5 > 100%

UV treated Outfall 101: Pimephalespromelas: IC,> 100%

Page 1 of 113

18. Facility

Contact:

Brad Love Phone#: (423) 843-6714

19. Consulting / Testing Lab: Environmental Testing Solutions, Inc.

20. Lab

Contact:

Jim Sumner Phone #: (828) 350-9364

21. TVA

Contact:

Donald W. Snodgrass Phone #: (256) 386-2787

22. Notes: Outfall 101 samples collected May 12 - 17, 2013, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were

> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 2 of 113

METHODS

SUMMARY

Samples:

1. Sampling Point: Outfall 101, Intake

2. Sample Type: Composite

3. Sample Information:

Date Date Date (MM-DD-YY) (MM-DD-YY) Arrival Initial (MM-DD-YY)

Sample Time (ET) Time (ET) Temp. TRC* Time (ET)

ID Collected Received (-C) (mg/L) Last Used By 101 05-12-13 0900to 05-13-13 1415 1.7, 1.6 ' <0.10 05-14-13 1345 05-13-13 0800 05-15-13 1246 05-12-13 0920 to 05-14-13 1345 Intake 05-13-13 0820 05-13-13 1415 1.8 <0.10 to 101 5-1-13075 5-1-13133 1.41.2 <0 10 05-15-13 1246 05-14-13 0750 05-16-13 1245 I01 05-14-13 0650to 05-15-13 1330 1.4, l.2t <0.10 05-16-13 1245 105-15-13 0050 05-17-13 1247 05-14-13 0805 to 05-16-13 1245 Itk 05-15-13 0705 05-15-13 1330 1.2 <0.10 05-17-13 1247 101 05-16-13 0650 to 05-17-13 1315 2.0, 1.5* <0.10 05-18-13 05-19-13 1245 1245 05-17-13 0550 05-20-13 1245 05-16-13 0705 to 05-18-13 1245 Intake 05-16-13 0705 05-17-13 1315 1.4 <0.10 05-19-13 1245 05-17-13 0605 05-20-13 1245

TRC = Total Residual Chlorine tSamples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature (25.0 +/- 1.00C) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 3 of113

Pitnephalespromelas Ceriodaphniadubia Test Organisms:

1. Source: Aquatox, Inc. In-house Cultures

2. Age: 21.5 - 21.75 hours8.680556e-4 days 0.0208 hours 1.240079e-4 weeks 2.85375e-5 months old < 24-hours old Test Method Summary:

1. Test Conditions: Static, Renewal Static, Renewal

2. Test Duration: 7 days Until at least 60% of control females have 3 broods

3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic

4. Number of Replicates: 4 10

5. Organisms per Replicate: 10 1

6. Test Initiation: (Date/Time)

Outfall 101 05-14-13 1345 ET 05-14-13 1045 ET UV Treated Outfall 101 05-14-13 1330ET

7. Test Termination: (Date/Time)

Outfall 101 05-21-13 1246 ET 05-21-13 0947 ET UV Treated Outfall 101 05-21-13 1230 ET

8. Test Temperature: Outfall 101: Mean = 24.7°C Mean = 24.9 0 C (24.1 - 25.00 C) (24.7 - 25.3 0 C)

Test Temperature: UV-Treated Outfall 101: Mean = 24.70 C (24.3 - 25.2 0C)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinnewille, CA).

Page 4 of 113

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

1. Results of a Pimephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 14 - 21, 2013 using effluent from Outfall 101.

Test Percent Surviving Solutions (time interval used - da s)

(% Effluent) 1 2213 4 5 6 7 Control *100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(% Effluent)i (replicate number) 1 2 3 4 Mean Control 0.629 0.687 0.563 0.620 0.625 10.8% 0.626 0.684 0.612 0.574 0.624 21.6% 0.601 0.642 0.602 0.545 0.598 43.2% 0.550 0.630 0.579 0.638 0.599 86.4% 0.560 0.617 0.595 0.606 0.595 100.0% 0.600 0.560 0.545 0.568 0.568 Intake 0.491 0.629 0.585 0.517 0.556 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.2%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50 : TUc = 100/IC 25 Page 5 of 113

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 14 - 21, 2013 using effluent from Outfall 101.

Percent Surviving Test (time interval used - days)

Solutions 1

(% Effluent) 1 2 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Test Soluetis Data (replicate number)

(%Effluent) 13 2 3 4 ] 5 6 7 I8 9 10 Mean Control 31 30 32 31 30 32 32 30 30 30 30.8 10.8% 33 30 31 33 33 33 32 30 33 33 32.1 21.6% 34 31 32 34 35 33 32 34 33 34 33.2 43.2% 36 34 36 33 33 34 35 34 33 34 34.2 86.4% 37 31 34 34 38 36 36 34 32 36 34.8 100.0% 35 38 37 34 36 35 36 38 36 37 36.2 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: 43.2%

Permit Limit: 2.3 TUc 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC5 0: TUc = 100/IC 25 Page 6 of 113

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets)

2. Results of a Ceriodaphniadubia Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 14 - 21, 2013 using water from Intake Percent Surviving Test (time interval used - days)

Solutions

(% Effluent) 1_ 2 3 4 I 5 6 7 Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100 Test Solutions Reproduction (#young/female/7 days)

Data (replicate number)

(%Effluent) 1i 2 3 4 5] 6 7 8 9 10 Mean Control 31 29 28 31 29 27 29 34 2 29 29.6 Intake 34 35 39 36 37 36 37 35 35.9 IC25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

Permit Limit: N/A Permit Limit: N/A 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50 : TUc = 100/IC 25 Page 7 of 113

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets)

3. Results of a Pimnephalespromelas Chronic/ 7-day Toxicity Test.

(Genus species) (Type / Duration)

Conducted May 14 - 21, 2013 using effluent from UV Treated Outfall 101.

Test Percent Surviving Solutions (time interval used - days)

(% Effluent) 1 2 ] 3 [ 4 5 J 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 10i 100 100 100 100 100 100 Test Solutions Mean Dry Weight (mg)

(%.Effluent) (replicate number)

(Eflet 1 2 ] 3 ] 4 Mean Control 0.593 0.548 0.575 0.594 0.578 10.8% 0.559 0.559 0.543 0.511 0.543 21.6% 0.575 0.584 0.618 0.592 0.592 43.2% 0.525 0.573 0.578 0.544 0.555 86.4% 0.532 0.596 0.715 0.615 0.615 100.0% 0.579 0.559 0.605 0.633 0.594 Intake 0.523 0.607 0.565 0.485 0.545 IC 25 Value: > 100% Calculated TU Estimates: < 1.0 TUc*

95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

TUa = 100/LC50 : TUc = 100/IC2 5 REFERENCE TOXICANT TEST RESULTS (see Appendix A and D)

Species Date [Time [Duration Toxicant Results (IC 25 )

Pimephalespromelas May 14 - 21,2013 1500 7 days KC1 0.78 g/L Ceriodaphniadubia May 07 - 14, 2013 1014 7 days NaC1 1.06 g/L Page 8of 113

PHYSICAUCHEMICAL

SUMMARY

Water Chemistry Mean Values and Ranges fbr Pimephalespromelas and Ceriodaphniadubia Tests, Non-treated Sequoyah Nuclear Plant (SQN) Outfall 101 (D performed May 14-21, 2013.

0 Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (Itmhos/cm) (mg/L CaCO 3 ) (mg/L CaCO3 ) Chlorine (mg/L) 24.8 24.5 7.7 7.5 7.95 7.74 310 61 87 Control 24.7 - 24.8 24.3 - 24.7 7.6 - 7.8 7.2 - 7.8 7.89 - 8.00 7.64 - 7.81 307 - 314 61 - 62 84 - 88 24.5 7.8 7.5 7.95 7.71 286 10.8% 24.8 24.7 - 24.8 24.3 - 24.8 7.7 - 7.9 7.2 - 7.9 7.90 - 7.97 7.66 - 7.75 283 - 290

.6 21.6% 24.8 24.5 7.8 7.5 7.95 7.69 270 24.7 - 24.8 24.2 - 24.7 7.7 - 7.9 7.2 - 7.9 7.90 - 7.98 7.63 - 7.77 267 - 272 94 24.8 24.5 7.8 7.5 7.93 7.68 234 43.2%

,?z _ 24.7 - 24.9 24.2 - 24.7 7.7 - 7.9 7.3 - 7.9 7.90 - 7.98 7.59 - 7.76 231 - 237

6. 24.9 24.5 7.8 7.5 7.87 7.66 160 86.4%

24.8 - 24.9 24.2 - 24.7 7.8 - 7.9 7.2 - 7.9 7.83 - 7.97 7.60 - 7.73 154 - 168 100.0% 248-25000.-20%

24.9 24.6 7.9 7.5 7.84 7.64 131 53 55 9 01 -< 0.10<01 24.8 - 25.0 24.4- 24.7 7.8 - 8.0 7.2 - 8.0 7.79 - 7.89 7.59 - 7.72 126 - 137 51 - 55 52 58 <0.10- <0.10 24.4 8.0 7.4 7.84 7.66 131 53 56 < 0.10 Intake 24.9 24.8 - 25.0 24.1 - 24.6 7.8 - 8.0 7.0 - 8.0 7.80 - 7.89 7.59 - 7.74 123 - 138 51 - 56 54 - 58 <0.10 - <0.10 24.8 25.0 7.7 7.8 7.95 7.93 310 61 87 Control 24.7 - 24.9 24.8 - 25.2 7.6 - 7.8 7.8 - 8.0 7.89 - 8.00 7.87 - 7.96 307 - 314 61 - 62 84 - 88 24.8 25.0 7.8 7.8 7.95 7.94 286 10.8%

24.7 - 25.0 24.8 - 25.3 7.7 - 7.9 7.8 - 8.0 7.90 - 7.97 7.88 - 7.98 283 - 290 24.8 25.0 7.8 7.9 7.95 7.94 270 21.6%

24.7 - 25.0 24.8 - 25.1 7.7 - 7.9 7.8 - 8.0 7.90 - 7.98 7.88 - 7.99 267 - 272

.* 43.2% 24.9 24.9 7.8 7.9 7.93 7.94 234 24.7 - 25.0 24.7 - 25.0 7.7 - 7.9 7.8 - 8.0 7.90 - 7.98 7.88 - 8.00 231 - 237

86.4% 24.9 25.0 7.8 7.9 7.87 7.92 160 24.8 - 25.1 24.8 - 25.1 7.8 - 7.9 7.8 - 8.0 7.83 - 7.97 7.83 - 7.97 154 - 168 100.0% 25.0 24.9 7.9 7.9 7.84 7.90 131 53 55 < 0.10 24.8 - 25.3 24.8 - 25.1 7.8 - 8.0 7.8 - 8.0 7.79 - 7.89 7.83 - 7.98 126 - 137 51 - 55 52 - 58 <0.10 - <0.10 Intake 25.0 24.9 8.0 8.0 7.84 7.89 131 53 56 < 0.10 24.9 - 25.2 24.8 - 25.0 7.8 - 8.0 7.9 - 8.1 7.80 - 7.89 7.81 - 7.97 123 - 138 51 - 56 54 - 58 <0.10 - <0.10 Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.7 24.1 25.0 Ceriodaphniadubia 24.9 24.7 25.3

-o CD 0 PHYSICAL/CHEM ICAL SUM MARY 0 Water Chemistry Mean Values and Ranges fbr Pimephalespromelas Test, UV-treated Sequoyah Nuclear Plant (SQN) Outfall 101 performed May 14-21, 2013.

-L CA)

Test Sample ID Temperature (C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness *Total Residual Initial Final Initial Final Initial Final (pmhos/cm) (mg/L CaCO 3) (mg/L CaCO 3) Chlorine (mg/L) 24.6 7.8 7.4 7.94 7.72 305 62 88 Control 24.8 24.7 - 24.9 24.5 - 24.7 7.7 - 7.8 7.2 - 7.8 7.89 - 7.96 7.67 - 7.81 301 - 311 61 - 62 86 - 92 24.6 7.9 7.4 7.94 7.72 287 10.8% 24.9 24.8 - 25.0 24.3 - 24.7 7.8 - 8.0 7.3 - 7.9 7.90 - 7.97 7.68 - 7.80 281 - 294 24.5 7.9 7.4 7.94 7.71 272 21.6% 24.9 24.8 - 25.0 24.3 - 24.8 7.8 - 8.0 7.2 - 7.9 7.89 - 7.97 7.67 - 7.76 269 - 274 Q- 43.2% 24.9 24.5 7.9 7.4 7.92 7.68 235 24.8 - 25.0 24.4 - 24.7 7.9 - 8.0 7.2 - 7.9 7.85 - 7.97 7.57 - 7.77 232 - 239 86.4% 24.9 24.6 8.0 7.4 7.87 7.63 161 1 24.8 - 25.0 24.3 - 24.8 7.9 - 8.0 7.1 - 7.9 7.79 - 7.96 7.54 - 7.72 156 - 167 24.5 8.0 7.4 7.83 7.63 132 53 58 < 0.10 100.0% 25.1 24.8 - 25.2 24.3 - 24.7 7.9 - 8.0 7.1 - 7.9 7.78 - 7.89 7.55 - 7.71 127 - 140 51 - 56 56 - 62 <0.10 - <0.10 Intake 25.0 24.5 8.1 7.4 7.82 7.68 131 53 56 < 0.10 24.8 - 25.2 24.4 - 24.7 8.0 - 8.1 7.0 - 7.9 7.75 - 7.88 7.65 - 7.77 125 - 139 50 - 56 54 - 58 <0.10 - <0.10

Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

Overall temperature (C) Average Minimum Maximum Pimephalespromelas 24.7 24.3 25.2

SUMMARY

/ CONCLUSIONS Outfall 101 samples collected May 12 - 17, 2013, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were

> 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 11 of 113

Appendix A ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY

OF METHODS

1. Pimephalespromelas Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphniadubia Tests were conducted according to EPA-82 1-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL

1. Pimephalespromelas None

2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS

1. Pimephalespromelas None

2. Ceriodaphniadubia None Page 12 of 113

PHYSICAL AND CHEMICAL METHODS

1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B-2000.

4. Dissolved oxygen was measured by SM 4500-0 G-2001.

5. The pH was measured by SM 4500-H+ B-2000.

6. Conductance was measured by SM 2510 B-1997.

7. Alkalinity was measured by SM 2320 B-1997.

8. Total hardness was measured by SM 2340 C-1997.

9. Total residual chlorine was measured by ORION 97-70-1977.

QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-82 1-R-02-013. Any known deviations were noted during the study and are reported herein.

During the Quality Assurance review of this report it was noted that the reported Average Flow on Days Sampled (MGD) for the third sample set (26,554.02 MGD) was an order of magnitude higher than expected or previously reported values.

Investigations revealed that the technician overseeing the sample collection had used river flow data instead of outfall flow data to determine average flow. Consequently, these flow data were also used in determining the volumes from the 24 aliquots used to prepare the composite sample for this final set. TVA acknowledges that this event was an unintentional deviation from the NPDES Permit requirements. However, calculations using the correct data were completed and compared with those used for the third composite. The average error per aliquot was 1.27% with an error range of 0.02% to 13.74%. Upon review of this information by TVA Quality Assurance personnel, it was determined that the WET test results were not compromised as the result of the sampling error. Corrective actions are being implemented to prevent this type of error from occurring in the future.

Page 13 of 113

REFERENCE TOXICANT TESTS (See Appendix D for control chart information)

1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl.

2. Standard Toxicant: Potassium Chloride (KCI crystalline) for Pimephalespromelas.

Sodium Chloride (NaCI crystalline) for Ceriodaphniadubia.

3. Dilution Water Used: Moderately hard synthetic water.

4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 14 of 113

REFERENCES

1. NPDES Permit No. TN0026450.

2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-82 I -R-02-013 (October 2002).

3. Standard Methods for the Examination of Water and Wastewater, 2 2 nd Edition, 2012.

4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 15 of 113

Sequoyah Nuclear Plant Biomonitoring May 14- 21, 2013 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks During Toxicity Test Sampling Page 16 of 113

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 17, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 03/12/1998 0.016 - - -

03/13/1998 0.015 - - -

03/14/1998 0.013 - - - - - -

03/15/1998 0.030 - - - - - -

03/16/1998 0.013 - - - - - -

03/17/1998 0.020 - - - - - -

03/18/1998 0.018 - - - I -

09/08/1998 0.015 0.014 0.005 - - 0.021 09/09/1998 0.003 0.031 0.011 - - -

09/10/1998 0.014 0.060 0.021 - - -

09/11/1998 0.013 0.055 0.019 - - -

09/12/1998 < 0.001 0.044 0.015 - - -

09/13/1998 < 0.001 0.044 0.015 - -

09/14/1998 0.008 0.044 0.015 02/22/1999 < 0.001 - -

02/23/1999 0.005 02/24/1999 0.009 02/25/1999 0.012 02/26/1999 0.008 02/27/1999 < 0.001 02/28/1999 < 0.001 - -

08/18/1999 - 0.015 0.069 0.024 0.006 -

08/19/1999 - 0.012 0.068 0.024 - --

08/20/1999 - 0.023 0.070 0.024 - 0.120 -

08/21/1999 - 0.022 0.068 0.024 - --

08/22/1999 - 0.022 0.068 0.024 --

08/23/1999 - 0.025 0.068 0.024 0.006 -

08/24/1999 - 0.016 0.067 0.023 0.020 -

Page 17 of 113

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 17, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/31/2000 - < 0.002 0.026 0.009 02/01/2000 - 0.011 0.026 0.028 -

02/02/2000 - 0.028 0.026 0.009 0.006 02/03/2000 - 0.008 0.027 0.009 - -

02/04/2000 - 0.006 0.027 0.009 0.005 0.109 02/05/2000 - < 0.002 0.027 0.009 02/06/2000 - < 0.002 0.027 0.009 -

07/26/2000 - < 0.0057 0.055 0.019 -

07/27/2000 - 0.019 0.055 0.019 -

07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 07/29/2000 - < 0.0088 0.055 0.019 - -

07/30/2000 - < 0.0076 0.055 0.019 -

07/31/2000 - < 0.0152 0.055 0.019 0.006 08/01/2000 - < 0.0141 0.055 0.019 0.005 12/11/2000 - 0.0143 0.025 0.020 0.005 12/12/2000 - 0.0092 0.025 0.020 0.005 12/13/2000 - < 0.0120 0.025 0.020 -

12/14/2000 - < 0.0087 0.025 0.020 -

12/15/2000 - 0.0120 0.025 0.020 0.005 12/16/2000 - < 0.0036 0.025 0.020 -

12/17/2000 - < 0.0036 0.025 0.020 08/26/2001 - 0.017 0.06 0.021 0.006 08/27/2001 - <0.0096 0.06 0.021 0.005 0.021 08/28/2001 - <0.0085 0.06 0.021 - -

08/29/2001 - <0.0094 0.059 0.020 0.005 0.021 08/30/2001 - <0.0123 0.06 0.021 0.005 -

08/31/2001 - <0.005 0.059 0.020 -

11/25/2001 - <0.0044 - - -

11/26/2001 - <0.0119 0.024 0.02 0.005 11/27/2001 - 0.0137 0.023 0.019 0.007 11/28/2001 - <0.0089 0.022 0.019 0.006 11/29/2001 - 0.0132 0.024 0.02 0.007 11/30/2001 -< 0.0043 0.024 0.02 12/09/2001 - <0.0042 - -

12/10/2001 - <0.0042 12/11/2001 - <0.0104 - - -

12/12/2001 - 0.0128 0.024 0.02 0.008 12/13/2001 - <0.0088 0.024 0.02 -

12/14/2001 - 0.0134 0.024 0.02 0.007 Page 18 of 113

Table B- 1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998- May 17, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 01/02/2002 -< 0.0079 0.023 0.02 0.006 -

01/03/2002 -< 0.0042 0.023 0.014 --

01/04/2002 - 0.0124 0.024 0.014 0.009 -

01/05/2002 -< 0.0042 - -- -

01/06/2002 -< 0.0042 - - -

01/07/2002 -< 0.0089 0.024 0.014 0.006 - -

02/24/2002 - < 0.004 - - -

02/25/2002 -< 0.004 0.023 0.023 -- -

02/26/2002 - 0.0143 0.023 0.023 0.007 - -

02/27/2002 -< 0.0041 0.023 0.023 - - -

02/28/2002 -< 0.0041 0.024 0.008 - -

03/01/2002 - < 0.0041 0.024 0.008 - -

05/05/2002 - - - - - -

05/06/2002 - - 0.058 0.02 0.014 -

05/07/2002 - - 0.058 0.02 0.015 -

05/08/2002 - - 0.056 0.019 - -

05/09/2002 - - 0.057 0.02 0.014 -

05/10/2002 - - 0.056 0.019 - -

08/04/2002 - <0.0058 - - -

08/05/2002 - <0.0058 0.053 0.018 - - 0.025 08/06/2002 - 0.0092 0.053 0.018 - --

08/07/2002 - <0.0107 0.055 0.019 0.007 -

08/08/2002 - <0.0061 0.055 0.019 -

08/09/2002 - 0.0152 0.054 0.018 0.008 10/06/2002 - <0.00497 - - - - -

10/07/2002 - 0.0153 0.054 0.018 0.009 - -

10/08/2002 - <0.0092 0.054 0.018 0.007 - -

10/09/2002 - 0.0124 0.053 0.018 0.009 - -

10/10/2002 - 0.0134 0.054 0.018 0.009 - -

10/11/2002 - <0.0042 0.054 0.018 - - -

01/12/2003 - <0.0035 - - - - -

01/13/2003 - <0.006 0.025 0.019 0.009 - -

01/14/2003 - <0.0118 0.026 0.020 01/15/2003 - <0.0063 0.026 0.020 0.009 01/16/2003 <0.0034 0.026 0.020 01/17/2003 <0.0034 0.026 0.009 -

04/06/2003 <0.0073 - --

04/07/2003 <0.0189 - 0.021 -

04/08/2003 <0.0117 - 0.021 04/09/2003 <0.0139 - 0.021 0.016 04/10/2003 <0.0113 - 0.021 0.018 04/11/2003 - <0.0073 0.022 -

Page 19 of 113

Table B- I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998- May 17, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat- H-130M Hypochlorite mg/L mg/L mg/L mg/L PF mg/L mg/L TRC Phosphate Copolymer DMAD mg/L Quat TRC Azole 06/15/2003 -< 0.0045 - - - - -

06/16/2003 -< 0.0037 0.057 0.020 - - 0.022 06/17/2003 -< 0.0048 0.041 0.014 - - 0.024 06/18/2003 -< 0.0048 0.041 0.014 - - 0.024 06/19/2003 -< 0.0085 0.058 0.020 - - 0.025 06/20/2003 - < 0.0048 0.058 0.020 - - 0.025 08/03/2003 - <0.0050 - - - - -

08/04/2003 - <0.0050 0.058 0.020 - - -

08/05/2003 - <0.0051 0.057 0.020 - - 0.025 08/06/2003 - <0.0084 0.057 0.020 - - 0.025 08/07/2003 - 0.0129 0.057 0.020 - - 0.024 08/08/2003 - 0.0153 0.057 0.020 0.009 - -

10/05/2003 - <0.0043 0.057 0.020 - - -

10/06/2003 - <0.0043 0.057 0.020 - - 0.025 10/07/2003 - <0.0090 0.057 0.020 - - 0.025 10/08/2003 - <0.0106 0.057 0.020 - - 0.025 10/09/2003 - 0.0181 0.026 0.022 - - 0.025 10/10/2003 - 0.0183 0.026 0.024 0.009 - -

02/01/2004 - 0.0093 0.027 0.009 -

02/02/2004 - <0.0034 0.026 0.009 02/03/2004 - <0.0034 0.026 0.009 -

02/04/2004 - 0.0124 0.026 0.009 0.009 02/05/2004 - <0.0034 0.026 0.009 -

02/06/2004 - 0.0105 0.026 0.009 0.010 05/04/2004 - <0.0123 0.026 0.019 - - 0.025 05/05/2004 - <0.0 144 0.026 0.014 0.009 - 0.025 05/06/2004 - <0.0146 0.037 0.013 - - 0.025 05/07/2004 - 0.0227 0.058 0.020 0.009 - 0.025 05/08/2004 - 0.016 0.060 0.021 - - -

05/09/2004 - <0.0104 0.058 0.020 07/04/2004 - 0.0217 0.057 0.019 - -

07/05/2004 - <0.0085 0.057 0.020 0.009 - -

07/06/2004 - <0.0077 0.058 0.020 - - 0.031 07/07/2004 - 0.0252 0.056 0.019 - - 0.031 07/08/2004 - 0.0223 0.057 0.019 0.009 - -

07/09/2004 - 0.0182 0.057 0.020 0.009 - -

Page 20 of 113

Table B- 1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 17, 2013 Date Sodium Towerbrom PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M Hypochlorit mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L e TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L EO/PO TRC 11/07/2004 <0.0187 0.000 0.014 - -

11/08/2004 - <0.0192 0.047 0.030 - - - -

11/09/2004 - <0.0233 0.048 0.016 - - 0.041 11/10/2004 - <0.0149 0.047 0.016 - - 0.041 -

11/11/2004 - <0.0149 0.049 0.017 - - 0.043 -

11/12/2004 - <0.0253 0.048 0.017 - 0.042 02/06/2005 - <0.0042 0.028 0.010 [

02/07/2005 - <0.0116 0.028 0.010 0.007 02/08/2005 - <0.0080 0.028 0.010 I I 02/09/2005 - 0.0199 0.028 0.010 -

02/10/2005 - <0.0042 0.028 0.010 02/11/2005 - 0.0155 0.028 0.010 1 0.007 06/05/2005 - 0.0063 -

06/06/2005 - 0.0043 - 0.037 06/07/2005 - 0.0103 - - - 0.037 06/08/2005 - 0.0295 - - - 0.037 06/09/2005 - 0.0129 - - - -

06/10/2005 - 0.0184 - - -

07/17/2005 - 0.0109 0.026 0.009 -- -

07/18/2005 - 0.0150 0.026 0.009 - 0.036 07/19/2005 - 0.0163 0.026 0.009 - 0.036 07/20/2005 - 0.0209 0.026 0.009 - - 0.014 0.036 07/21/2005 - 0.0242 0.026 0.009 - - -

07/22/2005 - 0.0238 0.054 0.018 - 0.014 10/30/2005 - 0.0068 - - - -

10/31/2005 - 0.0112 - - - -

11/01/2005 - 0.0104 - - - - 0.035 11/02/2005 - 0.0104 - - - - 0.036 11/03/2005 - 0.0117 - - - - 0.036 11/04/2005 - 0.0165 - - - - 0.035 11/14/2005 - 0.0274 - - - -

11/15/2005 - 0.0256 -- -

11/16/2005 - 0.0234 -- -

11/17/2005 - 0.0231 -- -

11/18/2005 - 0.0200 -- -

11/19/2005 - 0.0116 - - - - - - I Page 21 of 113

Table B-I (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 -May 17, 2013 Date Sodium Towerbror PCL-222 PCL-401 CL-363 Cuprostat-PF H-130M Nalco H-150M MSW Hypochlorite mg/L mg/L mg/L mg/L mg/L mg/L 73551 mg/L 101 mg/L TRC Phosphate Copolymer DMAD Azole Quat mg/L Quat mg/L TRC S _ ____L _ __EO/PO -

Phosphate

_h

- 0.0055 - -

11/12/2006 11/13/2006 - 0.0068 - - 0.037 -

11/14/2006 - 0.0143 -- - 0.037 -

11/15/2006 - 0.0068 -- - 0.037 -

11/16/2006 - 0.0267 - 0.037 -

11/17/2006 - 0.0222 -- -- -

11/26/2006 - 0.0188 -

11/27/2006 - 0.0138 -

11/28/2006 - 0.0120 -

11/29/2006 - 0.0288 -

11/30/2006 - 0.0376 -

12/01/2006 - 0.0187 - -

05/28/07 - -- - 0.015 05/29/07 - - -- 0.036 0.015 05/30/07 - 0.0084 -- 0.017 0.036 0.015 05/31/07 - 0.0103 -- - 0.036 0.015 06/01/07 - 0.0164 -- 0.017 0.036 0.015 06/02/07 - 0.0305 -- - - 0.015 12/02/07 - 0.0241 - -

12/03/07 - 0.0128 -

12/04/07 - 0.0238 -

12/05/07 - 0.0158 -

12/06/07 - 0.0162 -

12/07/07 - 0.0175 -

04/13/08 - 0.0039 -

04/14/08 - 0.0124 -

04/15/08 - 0.0229 -

04/16/08 - 0.0143 -

04/17/08 - 0.0120 -

04/18/08 - 0.0149 -

10/26/08 - 0.0260 -

10/27/08 - 0.0151 - 0.017 -

10/28/08 - 0.0172 - - 0.041 -

10/29/08 - 0.0154 - 0.018 0.041 0.030 10/30/08 - - - - 0.041 0.030 10/31/08 - 0.0086 - 0.041 0.030 Page 22 of 113

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 17, 2013 Date Sodium Towerbrorn PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Hypochlorite mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 mg/L TRC mg/L Copolymer DMAD Azole Quat mg/L mg/L Quat mg/L TRC Phosph EO/PO Quat Phosphate ate 02/08/09 - 0.0197 - 0.017 -

02/09/09 - 0.0237 - 0.017 -

02/10/09 - 0.0104 - 0.021 -

02/11/09 - 0.0155 - 0.017 -

02/12/09 - 0.0106 - 0.017 02/13/09 - -- -

05/10/09 - 0.0129 -.....

05/11/09 - 0.0415 -- - 0.0446 -

05/12/09 - 0.0053 -- - 0.0396 -

05/13/09 - 0.0049 -- - 0.0396 -

05/14/09 - <0.0141 -- - 0.0397 -

05/15/09 - <0.0160 -- -- -

11/15/09 - 0.025 -

11/16/09 - 0.0152 -

11/17/09 - 0.0255 11/18/09 - 0.0306 11/19/09 - 0.0204 11/20/09 - 0.0093 05/09/10 - 0.0192 -

05/10/10 - 0.0055 - - -

05/11/10 - 0.0100 -- 0.039 -

05/12/10 - 0.0171 -- 0.039 -

05/13/10 - 0.0041 - - 0.039 -

05/14/10 - 0.0099 - - 0.039 -

Page 23 of 113

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test Sampling, March 12, 1998 - May 17, 2013 Date Sodium Towerbrom PCL- PCL-401 CL-363 Cuprostat H-130M Nalco Spectrus H-150M MSW Floguard Hypo- mg/L 222 mg/L mg/L -PF mg/L mg/L 73551 CT1300 mg/L 101 MS6236 chlorite TRC mg/L Copoly- DMAD Azole Quat mg/L mgPL Quat mg/L mg/L mg/L Phos- mer EOCPO Quat Phos- Phosphate TRC phate phate 10/31/10 - - - -

11/01/10 - 0.0122 - --

11/02/10 - 0.0112 - --

11/03/10 - 0.0163 - --

11/04/10 - 0.0107 - - -

11/05/10 - 0.0132 -- -

05/01/2011 -- -

05/02/2011 - 0.04 - - -

05/03/2011 - - - 0.04 - - -

05/04/2011 - 0.0155 -- - 0.04 - - -

05/05/2011 - 0.0179 -- - 0.04 - - -

05/06/2011 - 0.0089 - - - -

11/06/2011 - 0.0168 - -

11/07/2011 - 0.0225 - -

11/08/2011 - 0.0141 -- -

11/09/2011 - 0.0239 -- -

11/10/2011 - 0.0242 -- -

11/11/2011 - 0.0231 - -

05/06/2012 - - - -

05/07/2012 -- -

05/08/2012 - - - - - - 0.041 -

05/09/2012 - 0.0145 - - - - 0.041 -

05/10/2012 - 0.0298 - - - - 0.041 -

05/11/2012 - 0.0174 - I - - - -

08/12/2012 - - - - - - - - - 0.029 08/13/2012 - 0.0256 - - - - 0.028 0.037 - - 0.029 08/14/2012 - 0.0209 -- - - 0.037 - - 0.029 08/15/2012 - 0.0279 -- 0.028 - - - 0.029 08/16/2012 - 0.0076 -- - - - - 0.029 08/17/2012 - 0.0446 - - - 0.032 05/12/2013 - 0.0099 - - -

05/13/2013 - - - - 0.064 05/14/2013 - 0.0091 - - 0.039 - - 0.064 05/15/2013 - 0.0096 -- 0.039 - - 0.064 05/16/2013 - 0.0229 -- - - - - 0.032 05/17/2013 - 0.0063 - - - I- I - - 0.032 Page 24 of 113

Sequoyah Nuclear Plant Biomonitoring May 14-21,2013 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets Page 25 of 113

BIOMONITORING CHAIN OF CUSTODY RECORD Pagc -I- of -I-

'U (0 Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

0 FedEx UPS Bus Client Project Name: Sequoyah NP Toxicity 351 Depot Street.

0

-I' P.O. Number: N/A Asheville, NC Other (specify):_.62x , * /

General Comments: P Facility Sampled: Sequoyah NP 28801 NPDES Number: TN0264&, k j Phone: 828-350-9364 Collected By: &,r!"" '11 Fax: 828-350-9368

-- a_-

Container W'i,*,

eqOutA Field Identification / Grab/Cmp. Coltection Date/Time Number & Flow Rain Event?

Sample Description Volume (MGD) pLaboratory Use Collected (Mark as Appropriate)

-,.<,.i.."".* Stte Start End S If Yes' N No NoQ Trace ETS Log Arrival Temp. By Time Appear-

.7.*

i* .. .'-'.. ' . _ _,_t..,h.*'*e%. ," ,* ..- *hcI- Number (°C) .X" ance SQN-101-TOX- '-t:omp 2(2.5gal) I"/%o0.q'i / I.o::3l . 4 SQN-fNT-TOX - Comp 1(2.5 gal)

Sample Custody - Fill In From Top Down 1 L'WAro4 SeLS 6i TA

,aalCV. 5 ^"ILe.

Received nature): Date/T'ime100. T.LL ftiýt1 Relinquish d By (Signature): Date/Time So* 'v rr

A .. ...... : //'

/

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6VC and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that tinte frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Thit IETS Whole Effluent Sample Receipt Log -Page (0

Sample temperature performed using Sample Receiving Thermometer. SN 122164697

~0 Date Time Received Received *Sample Project Sample Sample name and description State Comments Received Received by from Temp. (OC) number number N 05-13-13 1415 J. Sumner TVA Courier 1.7/1.6 8968 130513 .01 TVA - Sequoyah Nuclear- 101 TN 0 05-13-13 1415 J. Sumner TVA Courier 1.8 8968 130513 .02 TVA - Sequoyah Nuclear - Intake TN 05-13-13 1612 J.Sumner TVA Courier 1.8 8969 130513 .03 WIVA-Watts Bar- 101 TNI 05-13-13 1612 J. Sumner TVA Courier 2.2 8969 130513 .04 TVA - Watts Bar- 101 Intake TN 05-13-13 1612 J. Sumner TVA Courier 2.1 8969 130513 .05 TVA-Watts Bar- 113 TN 05-13-13 1612 J. Sumner TVA Courier 2.5 8969 130513 .06 TVA - Watts Bar- 113 Intake TN SOP G4 - Exhibit G4.2, revision 01-03-12

BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of -I-(D Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

l Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client P.O. Number: N/A Asheville, NC Other (specify): 5Ok,. bell'uevy Facility Sampled: Sequoyah NP 28801 General Comments:

7# lot Phone: 828-350-9364 NPDES Number: TN0026450 Collected By. Fax: 828-350-9368 Container pr',0jk

eii ( i5 Field Identification Grab/Comp. Collection DateiTiie Number & Flow Rain Event?

Sample Description Volume (MGD) (Mark as Appropriate) Laboratory Use Collected

,

:-.t*" ' ~ f YetrsEd.~"' N rc ETS Log Arrival Temp. By Time Appear-

-.. - *.* ' ., Inches CNumber - ance SON-101-TOX~- ) Camp i[I'{ &o l. tf0l1 atq ¢,. ,*O 4

-¢og/q ,* "-*,'V t,* ,'

2.

.(2.Sgal) Aa't,*I

/

SQN-1NT-TOX-. b Comp V3OsiSOl A I Sample Custody - Fill In From Top Down -" . , *.#Lr Sfta.w. wtet.tJ Relinquished By (Signature): Date/Time Received B (Signature): Date/Time SAK4AS

-rip. O!'1 '3E

.~O 1%.--- - vs-,0-,5c " Eo

(

/

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples arc preserved by storing them at 60C and shipping them in ice. The hold time for each sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therefore, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samples shipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

Page .. __

j%-1 TS Whole Effluent Sample Receipt Log

Sample temperature performed using Sample Receiving Thermometer. SN 122164697

%w Date lime Received Received *Sample Project Sample (D Sampe nd escrptin nme Sate Comments Received Received by from Temp. ('C) number number 40 05-15-13 0931 K.Keenan Fed - Ex 1.1 8979 130515 .01 Bladenboro WWVTP NC 0

05-15-13 0931 K.Keenan Fed - Ex 0.5 8980 130515.02 Craven County Wood Energy NC _

05-15-13 0931 K. Keenan Fed - Ex 1.9 8981 130515 .03 Dallas WWTP NC 05-15-13 0931 K.Keenan Fed - Ex 1.0 8982 130515 .04 North Cary WWTP NC 05-15-13 0931 K.Keenan Fed - Ex 0.5 8983 130515 .05 Progress Energy - Shearon Harris E & E NC 05-15-13 0931 K.Keenan Fed - Ex 1.0 8984 130515 .06 Progress Energy - Shearon Harris NC 05-15-13 1009 K. Keenan UPS 0.6 8985' 130515 .07 South Cary WWTP NC 05-15-13 1330 J. Sumner TVA Courier 1.4/1.2 8968 130515 .08 TVA - Sequoyah Nuclear Plant - 101 TN 05-15-13 1330 J. Sumner TVA Courier 1.2 8968 130515 .09 TWA - Sequoyah Nuclear Plant - Intake TN 05-15-13 1630 J. Sumner TVA Courier 0.9 8969 130515 .10 TVA - Watts Bar- Outfall 101 TN 05-15-13 1630 J. Sumner TVA Courier 0.7 8969 130515 .11 TVA- Watts Bar- Intake -10 TN 05-15-13 1630 J. Sumner TVA Courier 1.8 8969 130515 .12 TVA - Watts Bar - Outfall 113 TN 05-15-13 1630 J. Sumner TVA Courier 1.3 8969 130515 .13 TVA- Watts Bar- Intake 113 TN_

SOP G4 - Exhibit G4.2, revision 01-03-12

RIOMONITORING CHAIN OF CUSTOI)Y RECORD Page 1 of -11I

.1 F U U

Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One).

CD Fedl3x UPS Bus Client CA~ Project Name: Sequoyah NP Toxicity 351 Depot Street.

0 P.O. Number: NIA Asheville, NC I (A~ General Comments:

Facility Sampled: Sequoyah NP 28801 NPDES Number, T"N0026W50 Phone: 828-350-9364 V.,//;-*-Wl - LV Collect Fax: 828-350-9368 2e

- . I i -

Ficld ldentificalior /

Container e.t, L 09L1 Number & Flow Rain Evemnt?

Grnb'Cump. Cllection lateJ'rimc Sample tlcacriplion Volume (MGD) Laboratory Use (Ma,'k as Appropriate)

Collected 3-"*jl -IT. , 7" t I!- MIart SQN- I L) I -'lOX Com o5Ol 2( U 16 CoI d59/5T ___ Is~zI __

0o 10 -V 4& 0 2) E~-

SQN-INT-TOX Comp 1(2.5 gal) '-'Ir 0-0V/ 1 o/ib3 I I~ ___________ L Sample Custody -- Fill In From Top l)own I I Relinquished By (Signature): Date/Time Received By (Signature):

I V Th

/t 1,?

v 1 4 4 V 1

________________________________________________________________ J Instructions: Clients should rill in all areas except those in the "Laboratory Use' block. Biomonitoring samples are prescrvyct by storing them at 6'C and shipping thcm iii ice. The hold time foreach sample is 36 hours4.166667e-4 days 0.01 hours 5.952381e-5 weeks 1.3698e-5 months from the time of collection. Therctbre, please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing withiin that time frame. Samples shipped overnight on [ridlay via FedEx or UPS niust be mauked for Saturduy delivery or they will not arrive until the following Mondny.

~ff I O:ETS Whole Effluent Sample Receipt Log Page.!

Sample temperature performed using Sample Receiving Thermometer; SN 122164697 (D Date Time Received Received *Sample Project Sample (4) Received Received by from Temp. (0 C) number number Sample name and description State Comments 05-17-13 0935 K. Keenan UPS 0.7 8985 130517.01 South Cary WWTP NC 0

05-17-13 0940 K.Keenan Fed - Ex 1.3 8979 130517 .02 Bladenboro WWTP NC 05-17-13 0940 K. Keenan Fed - Ex 3.4 8980 130517 .03 Craven County Wood Energy NC I (A) 05-17-13 0940 K.Keenan Fed - Ex 1.8 8981 130517.04 Dallas WWTP NC 05-17-13 0940 K. Keenan Fed - Ex 2.2 8982 130517 .05 North Cary WWTP NC 05-17-13 0940 K. Keenan Fed - Ex 2.7 8987 130517 .06 Duke Energy - McGuire NS - Outfall 002 NC 05-17-13 1315 J. Sumner TVACourier 2.0/1.5 8968 130517.07 TVA - Sequoyah Nuclear Plant - 101 TN 05-17-13 1315 J. Sumner TVA Courier 1.4 8968 130517.08 TVA - Sequoyah Nuclear Plant- Intake TN 05-17-13 1517 1. Sumner TVA Courier 2.5 8969 130517.09 TVA- Watts Bar - Outfall 101 TN 05-17-13 1517 J. Sumner TVA Courier 2.3 8969 130517.10 TVA-Watts Bar- Intake 101 TN 05-17-13 1517 J. Sumner TVA Courier 2.6 8969 130517 .11 TVA - Watts Bar - Outfall 113 TN 05-17-13 1517 J. Sumner TVA Courier 2.1 8969 130517 .12 TVA - Watts Bar - Intake 113 TN SOP G4 - Exhibit G4.2, revision 01-03-12

Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: Tennessee Valley Authority County: M lAW uL1 Facility: SequoyahNuclearPlant Outfall: 101 NPDES #.t* *60 1 o611ASO Project#: ___

Dilutionpreparationin ormation: Comments:

Dilution prep 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:

Organism age: 2_.1 "t*Adj.C o0.. Randomizing template: %le.utloi Date and times organisms o0 '%. 0b Incubator number and were born between: shelf location: ______..

Organism source: Ar(. ,Aiu e Artemia CHM number: C1'L.

Drying informationfor weight determination:

Transfer bowl information: pH = S.U. Date / Time in oven: 0L2-L%. iS Temperature = "Lo'* °C Initial oven temperature:

Average transfer volume: Date/ Time out of oven: O2., t$

(&

. l¢ Final oven temperature: 60 .

Total drying time: "L4lkbA-Dailyfeeding and renewalinformation:

Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 1 ,040 ... 'L.. ttAqL o_IS.l \'SOlb.(2. -I1 4

I2 5_ osl.14.3 is 04S .A.. 1{. 2S A'I N0S 0L. -d01 vbosm1Of 6S.11. I' 6

OS-"20.1 V iqlc& I-)tLI4 t'heir tk1. 01 I'Aul11. OF rm. il* amA 7 . ro, I -

Controlinformation: Acceptance criteria Summary of test endpoints:

% Mortality: 6)1. :520% 7-day LC50 Average weight per initial larvae: O.hl.S NOEC I 0{ .

Average weight per surviving larvae: O.66 > 0.25mg/larvae LOEC -j,1007.

ChV *,loi IC25 >>1007.

SOP AT20 - Exhibit AT20.3, revision 07-01-12

- age 32 of 113

0:TS Page 2 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant. Outfall 101, Non-treated Date: ot- A, M Survival and Growth Data Day CONTROL 10.8% 21.6%

A B C D E F0 G H I K L (0

0L Q (0 10' 1 1 10 10(,1/0 1 ( 1 1 01 It) 1 I6 100 /0 1 0 /0 2 1(10) t10 ) t1 tO 10 to to(0o Ito 3 /0 i0 1 Ito ( I0 Io 10 ( 10 10 1t /0 4 I() 10 1o I to

_ _to to 10 to (0 10 10 (..

5 toI___10 UQ10i of tco t t0 I 0 ')3o,0'0 6 () / 1) I t ) IC tI I0Ct) 1.0 ' 0 .110 0 I I0) 101010/016 ) ,/o ,0/0 A Pan weight (mg)

Tray color code:: ,, .. .I l/. . .7r6 J q Date oS-ol Z13

.SI

.l.3 6516q .7q 13.5 1~3.7 V.513 3V Date:

Analyst: *LI :1M .- S.,..

1 CJZ.00p q 13 N 562.215 lIIq C =Larvae weight (mng) =B - A Hand calculated.

Weight per initial number of larvae (mg)

= C / Initial number of larvae 1% '9 'V I', 'V N Hand calculated. '0 I,, "V k,

Analyst: 0 a 0 Average weight per Percent reduction 0.I. ' O o -~ -

Initial number of from control (%) I c larvae (mg) L Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

SOP AT20 - Exhibit AT20.3, revision 07-01-12 (Iage 33 of 113

DETS Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date: o*-*-4 i Survival and Growth Data Day 43.2% 86.4% 100%

M N 0 P Q R S T U V W X.

0 1I0 10 to 1 t o 10 to 1()c to 10 tO)1 1 It) 101to01 (0 /*o*to. 100/O 1 2 to IDC)I0I'() / I0( to I () I () O 3 /D to 10 10 ý1() 10 to I(3 I) I() to /c 24 pI t0 10 t0 lO 10 10 (0 11 5 t0 10 i*) /O 10 t/0 I 13 1 r0 0 (0 to 6 1 to to 101010 (0 to1 01 0 tOi OoC)o

'0 1 10 10 10 t0 1 tto 10 10 to 10 to A f Pan weight (mg) rjUL 3 q.Ozi Tray color code::

Analyst:

Date:_0____

___ 41-________________

I.31 q. 13.35 138g Iq.1? 13.71 I.01 130 q38 B = Pan + Larvae weight (mg)

Analyst: ______._,__0_. 7q 152 Z0,Wq q ,?l1171 Z0.ll 61Iqý 20,0(

C = Larvae weight (mng) = B - A Hand calculated. S.1%0 6S S.fl 1-t S.6 (..%I SAS q~16 06 6.I.O SMWSAiSS6 Analyst:

Weight per initial number of larvae (nmg)

=C /Initial number of larvae 0 "

Hand calculated. %a %

Analyst: 0#0 ,o Average weight per Percent reduction initial number of larvae (MR) from control (%) '4. 1l o .SO%5 A 7.- 9i

%-7 Comment codes: c = clear, d = dead, fg = fungus, k = killed,m = missing,sk = sick, sm= unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

coi ments.

SOP AT20 - Exhibit AT20.3, revision 07-01-12 Sage 34ofl113

IETS . ... *"Page 4 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, Non-treated Date: r-t%

Day 100% Intake Y_ _ __ Z AA BB o

0 ( (0 1(

1 10 10 /0 3

4 10 to 5 lo 1r0 10 10 6 5(0IO ,I 7 (

A = Pan weight (mg)

Tray color code:: r_ ___

Analyst: SIR j9 jL)

Date: O13.oq. 1l3 ..... .

B = Pan + Larvae weight (mg)

Analyst: _ V*- . L Date: 06.'2?-13 1 .q1 .731839.1 C = Larvae weight (mg) - B - A Hand calculated. q e .'2-£.SA S11 Analyst:

Weight per initial number of larvae (mg)

= C / Initial number of larvae Hand calculated. 4. L Analyst: _ .*-%.'-, -- . l.

=Average weight per Percent reduction initial number of from control S%) I. it$.17.

larvae m Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.

Comments.

SOP AT20 - Exhibit AT20.3, revision 07-01-12

- age 35 of 113

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 14-21,2013 PimephalespromelasChronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses SEnvironmental Testing Solutions, Inc. Project number. 8968 Not for Compliance Assessnmentt, Internal Laboratory QC Com.tuonn Rotp8le.

f(%) Jltinumbernof F1.lrmb-rat lm A-P..wg-fbt(m) B-Pa-+L.r- L-ro.wedolt(ig) Wert/so-linmg M-oeaetuhtl Codcidentorf-atiou Weight/la/idd number M cManuva MeMu.dightfltoilt Coeffiientot PFet.ed.ctoo fam tarae we ) - A-B aamberofla (mg) Somretg umbe, of W up', ortaruve(aw) (%)

M omberofluue ,.olon a&.__&0 centr. (0.)

A 10 10 14.90 21.19 6.29 0.629 0.629 B 10 10 14.50 21.37 6.87 0.687 0.625 8.1 0.637 Control 10 10 12.53 18.16 5.63 0.563 0.567 100.0 0.625 8.1 Not applicable D 10 10 13.80 20.00 6.20 0.620 0.620 E 10 10 13.19 19.45 6.26 0.626 0.626 F 10 10 15.65 22.49 6.84 0.684 0.684 G 10 10 13.81 19.93 6.12 0.612 0.612 H 10 10 14.79 20.53 5.74 0.574 0.574 I 10 10 13.55 19.56 6.01 0.601 0.601 J 10 o10 13.78 20.20 . 6.42 0.642 0.598 6.7 0.642 100.0 0.598 6.7 4.4 K 10 10 12.53 18.55 6.02 0.602 0.602 L 10 10 13.49 18.94 5.45 0.545 0.545 M 10 10 13.44 18.94 5.50 0.550 0.550

____ 10I. 0 421 2.160 _______0.630________

"0.63010.0597041 43.2% N 10 14.21 20. 6.3 0.630 0.599 7.0 100.0 0.599 7.0 4.1 o 10 10 14.60 20.39 5.79 0.579 0.579 P 10 10 14.39 20.77 6.38 0.638 0.638 Q 10 10 14.59 20.19 5.60 0.560 0.560 86.4% R 10 10 13.35 19.52 6.17 0.617 0.595 42 0.617 100.0 0.595 4.2 4.8 S 10 10. 13.88 19.83 5.95 0.595 0.595 T 10 10 14.12 20.18 6.06 0.606 0.606 U 10 10 13.71 19.71 6.00 . 0.600 0.600 100% V 10 10 15.01 20.61 5.60 0.560 0.568 4.1 0.560 100.0 0.868 4.1 9.0 W 10 10 13.90 19.35 5.45 0.545 0.545 X 10 10 14.38 20.06 5.68 0.568 0.568 Y 10 10 13.68 18.59 4.91 0.491 0.491 Z 10 10 13.44 19.73 6.29 0.629 0.556 11.3 0.629 100.0 0.556 11.3 11.1 AA 10 10 12.54 18.39 5.85 0.585 0.585 BB 10 10 13.01 18.18 5.17 0.517 0.517 Outfall 101: MSD= Minimumn Significant Difference Donnett's MSD value: 4.0666 PMSD = Percent Minimum Significant Difference PMSD: 10.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake:

Dunnett's MSD value: 0.0786 Lower PMSD bound determined by USEPA (I Othpercentile) = 12%.

PMSD: 12.6 Upper PMSD bound determined by USEPA (90th percentile) = 30%;

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA-s WET Interlaboratsoy Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2001a. 2001b. Final Report Interlaboratory Variability Study ofEPA Short-tenr Chronic and Acute Whole Effluent Toxicity Test Methods, Volunes I and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency. Cincinnati. OR.

File: sqn101_051413data.xlsx Entered by: J. Stoner Reviewed by-.

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 14-21, 2013 Statistical Analyses S ntment.sw SolI fon.. Inc.

Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5114/2013 Test ID: PpFRCR Sample ID:. IVA/SON 101 End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report

.Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.6290 0.6870 0.5630 0.6200 10.8 0.6260 0.6840 0.6120 0.5740 21.6 0.6010 0.6420 0.6020 0.5450 43.2 0.5500 0.6300 0.5790 0.6380 86.4 0.5600 0.6170 0.5950 0.6060 100 0.6000 0.5600 0.5450 0.5680 Intake 0.4910 0.6290 0.5850 0.5170 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CVh N t-Stat Critical MSD Mean N-Mean D-Control 0.8248 1.0000 0.6248 0.5630 0.6870 8.124 4 0.6248 1.0000 10.8 0.6240 0.9988 0.6240 0.5740 0.6840 7.314 4 0.027 2.410 0.0656 0.6240 0.9988 21.6 0.5975 0.9564 0.5975 0.5450 0.6420 6.673 4 0.986 2,410 0.0666 0.5984 0.9578 43.2 0.5993 0.9592 0.5993 0.5500 0.6380 7.003 4 0.923 2.410 0.0666 0.5984 0.9578 86.4 0.5945 0.9516 0.5945 0.5600 0.6170 4.153 4 1.095 2.410 0.0886 0.5945 0.9516 100 0.5683 0.9096 0.5683 0.5450 0.6000 4.085 4 2.045 2.410 0.0666 0.5683 0.9098 Intake 0.5555 0.8892 0.5555 0.4910 0.6290 11.345 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test Indicates normal distribution (p > 0.01) 0.96674 0.8B4 -0.0129 -0.5557 Bartlett's Test Indicates equal variances (p = 0.78) 2.49894 15.0863 Hypothesis Test (1-tallt 0.05) NOEC LOEC ChV TU MSDu MSDp MSS MSE F-Prob df Dunnett's Test 100 >100 1 0.06658 0.10657 0.00178 0.00153 0.36414 5,18 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL(Exp) Skew IC05 86.912 IC10 >100 IC15 >100 1.0.

IC20 >100 0.9 IC25 >100 IC40 >100 0.8 IC50 >100 0.7 o.8.

0.5-10.4 0.3 0.2 0.1 I 0 50 100 150 Dose %

Dose-Response Plot 0.8 0.7 0.8 1-tail, 0.05 level 0.5 of significance 90.4 t 0.3 0.2 0.1 8 0- N Fde: sqnlOI_051413data.xix Entered by:J. Sumner age 37 of 113 Reviewedby:

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 14-21, 2013 I"ETS nlon e~ Tetn Aoutions, Inc.

Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/14/2013 Test ID: PpFRCR Sample ID: IVA / SQN 101 - Intake End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: Non-treated Conc-% 1 2 3 4 D-Control 0.6290 0.6870 0.5630 0.6200 10.8 0.6260 0.6840 0.6120 0.5740 21.6 0.6010 0.6420 0.6020 0.5450 43.2 0.5500 0.6300 0.5790 0.6380 86.4 0.5600 0.6170 0.5950 0.6060 100 0.6000 0.5600 0.5450 0.5680 Intake 0.4910 0.6290 0.5850 0.5170 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.6248 1.0000 0.6248 0.5630 0.6870 8.124 4 10.8 0.6240 0.9988 0.6240 0.5740 0.6840 7.314 4 21.6 0.5975 0.9564 0.5975 0.5450 0.6420 6.673 4 43.2 0.5993 0.9592 0.5993 0.5500 0.6380 7.003 4 86.4 0.5945 0.9516 0.5945 0.5600 0.6170 4.153 4 100 0.5683 0.9096 0.5683 0.5450 0.6000 4.085 4 Intake 0.5555 0.8892 0.5555 0.4910 0.6290 11.345 4 1.712 1.943 0.0786 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92927 0.749 0.13206 -1.4604 F-Test indicates equal variances (p = 0.73) 1.54165 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.07862 0.12584 0.00959 0.00327 0.13782 1,6 Treatments vs D-Control Dose-Response Plot 0.8 0.7; 0.6-0.5

--- 1-tail, 0.05 level of significance 0

0 0.4-I0.3 0.2:

0.1 0

cq NRci I 0 o

N0 cM c6 C 6

File: sqnl0l 051413data.xlsx Entered by: J.Suiqner Reviewed by: _i Cage 38 of113

$ ** ..... PeTf Page 5 of 6 Species: Pimephalespromelas Date: C6 . r*

Client: TVA / Sequoyah Nuclear Plant. Outfall 101, Non-treated Datlv Chemsftr:

(Analyst identified for .each da,* performed Day pH, D.O. and conductivity measurements only.)

0 1 1 2 Analyst jul if *~rt~

Concentration IParameter oH (S.U.) 1.91 L.79 1 7.43 i117 1 -7.47 11-7.75 DO (mn/L) 7.7 7 Conductivity 1313 CONTROL (gmhos/cm) ____

Alkalinity 6 Non-treated (mg CaCO 3/L) ____

EE=

Ulm &W

Hardness (mng CaCO3 /L) ____

Temperature (00

_______________ - - a I DH (SU. 7. qc .q6 7.7 rDO (mg/L) IZ ii 10.8%

6 Conductivity (gmhoslcm)

Temperature (*C) nH (S.U.)

6

?A7" i

r--*

... ,4 -------- !!-

DO (m./L) 7oA 21.6% Conductivity %PIZ

Temperature ( C) 0 11., ZILARG Dl 1.

I1 U~ 7. z.. 7. 4 .

Iq U

DH (S.U.) %9G DO (mg/L) 1-47-8 7.1 1(I 43.2% Conductivity (jgmhosl) J9~I I - 'L31

Temnerature (°C) '51..

I--~&A 7 t~ a II I

pH (S.U.) 7.II 17.ac 11 1- C3 1 *7.193 DO (Min/ 41 &I 19 1I i~

157ii 7 86.4%

Conductivity

Temperature (0 C) "LA., *hA A Ii I 'I ~ ¶ ,. - - - - - .. - - -

oH (.U.L) 7.55

.4:----

7. CE I "Agi ii 7e~ .-

.?7q II II DO (m/UL)-- -

9.0 Conductivity (0imhos/cm) a~t

Alkalinity 100% (Mg CnCn 3/L) __

Hardness (TRgCaCo3/L) sic6

TR chlorine (mg/L), 0 ,.ID

Temperature (°C) 'M..0 7.0 i~

S - a~

PH (s.u.) 7.65 DO (ma/L) 4LO Conductivity

(;Mmhos/cm) _____

100% *Alkalinity Intake *Hardness

<eo (mg CaCOr/L)

TR chlorine (mg/L) j S I

40. 40

-2.. e

Temperature (C) "Lfl.%

U 6 - - -

Iniia Fial Initial l 'Final Initial B Final 1

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located7on Page 1.

iha'dness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet P 3 1SOP AT20 - Exhibit AT20.3, revision 07-01-12 fPage 39 of 113

SET " Page 6 of 6 Species: Pimenhales promelas Client: TVA / Seuuovah Nuclear Plant. Outfall 101. Non-treated Date: CA. t*t Client: TVA / Sequoyah Nuclear Plant. Outfull 101. Non-treated Date: os-I F

4 Day (Analyst identified for each day. nerfbrmed oH. D.O. and conductivity measurements only.)

I--------------------------------------------------------------

5 6 Analyst Concen- Parameter tration PH (S.U.) '7-eq II DO (mgt7.) 17.7 I

7.

Conductivity31 J311 CONTROL (jimhos/cm) '1 A:06 Non-treated (mugCaCO31L)

Hardness 1 (mugCaCO3IL) ' E~

Temnerature t') ~]hL I II i*k II pH S.U.) 7.47 If7go 7 7.4 -s 9q l7.V DO (mg1L) '717 77 13 1.7 lC 7.7 1L5 10.8% Conductivityj

Temperatuire (-C) -14. -LA. 6. 21L g bAA 4 pH (S.U.) 7.Tq -7." 7.q0 -7.C3 I7.C 7 DO (me./U -7 7"/7 1". "/7', -

V? 1.7 15.

21.6% DO (mg/L)

Conductivity1 (jrmhos/cm). I U7 C~ Vlo 16 11if?

U

Temperature (C)

U -

"lA A'

- --- dI~

"is S U Ih~ U oH (S.U.' 7.qg -7.q -7.A 1. 1e "lqL PH (SU_) 190 1-10 -7 W -79q, DO (mP/L) 7.9 -7.3 7.7 1.7 43.2% Conductivity (g hoskcm) ?-izZ ZS 3-7

Temperature ('C) ".. ii -lA.M I -t1 * "1*I. G, -I., 1

Temperature CC) Vk. f 11 ýL`I I -LOA'A 16---m6aiiia 'LkA -9 -Lq.(. U-1 Wý pH (S.U.) 7.9CO 74 1 793 7 1?3 7.713 7.M1 J DO (malL) 86.4% Conductivity (ptnhos/cm) 7*7 1468

Temperature (0C0

1**

7.2 1 7. 1C7 Us pH (S.U.) 1.71 7Z 7. -7.CC E

iO (omn./ 7.1 7. .

DO (m.5.

Conductivity (Remhos/cm) 1-3t t 37

Alkalinity 100% (mg CaCO 31L)

Hardness (mug CaCO3/L)

TR chlorine (mg/L)

Temperature (0C)

ISm nH (S.U)

PH (S-U-)

DO (m*U 4 Conductivity (iimhoslcm) 100% *Alkalinity Intake (mug CaCO3 /L)

Hardness (Mug COC0 3 /L)

TR chlorine (mig/L)

Temnerature (*QC

. .. .... .. . .. "pt ..... nt a "MF i al

eratures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.

W it, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet f a e40 0" of 1 113*** SOP AT20 -Exhibit AT20.3, "revision 07-01-12

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated 0May 14-21, 2013

o Pimephalespromelas Chronic Whole Effluent Toxicity Test (D EPA-821-R-02-013, Method 1000.0 4_ _ Daily Chemical Analyses Environmental

"( Testing Solutions, Inc. Project number: 8968 Concentration Parameter Day 0 Day 1 Day 2 Day 3 Day 4 Day5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final PH (SU) 7,93 7.78 7.93 7.72 7.971 7.75 7.97 7.64 7.89 7.69 8.00 7.79 7.93 7.81 DO (mg /L) 7.7 7.8 7.8 7.5 7.7 7.3 7.7 7.2 7.6 7.3 7.7 7.6 7.7 7.5 Control Conductivity Alkalinity (mg/L(gmhostcm)

CaCO3 ) 313 61 309 307 = 310 . 308 S 311 _* 314 M. O 62 ME . 61 61 .

Hardness (mg CaCO /L3) 84 1 ME .88 88 1 88 Temperature ('C) 24.8 24 6 24.8 24.7 24.8 24.5 24.8 24.6 24.8 24.4 24.7 24.7 24.7 243 PH (SU) 7.96 7.72 7.93 7.66 7.95 7.75 7.97 7.67 7.90 7.68 7.97 7.75 7.94 7.75 DO (mg/L) 7.9 7.9 7.8 7.5 7.8 7.3 7;7 7.2 7.7 7.3 7.7 7.6 7.7 7.5 10 .8 % Co n d u ctivity (munh os/e m ) 2 90 . 2 85 6. 287k4 28 286 .2 84 28 .. "

Temperature C'C) 24.8 24.5 24.8 24.4 24.81 24.8 24.8 24.6 24.8 24.4 24.8 24.4 24.7 24.3 PH (SU) 7.97 7.71 7.93 7.66 7.95 7.67 7.98 53 7.66 7.90 7.63 7.96 7.76 7.94 7.77 21.6% DO (mg/L) 7.9 7.9 7.8 7.4 7.8 7.3 7.8 7.2 7.7 7.3 7.7 7.6 7.7 7.5 Conductivity (pmhos/cm) 272 M 270 -268 267 269 270 271 .

286.

Temperature C'C) 24.8 24.5 24.8 24.7 24.8 24.7 24.8 24.4 24.8 24.5 24.8 24.4 24.7 24.2 PH (SU) 7.96 7.70 7.92 7.66 7.90 7.67 7.98 7.60 7.90 7.59 7.90 7.76 7.94 7.76 DO (mL) 7.9 7.9 7.8 7.4 7.8 7.3 7.8 7.3 7.8 7.3 297.7 E*"25 7.6 7.7 7.6 43.2% Conductivity (Rmhos/cm) 234

236 231 1 232 235 23 -235 Temperature 'Cc) 24.8 24.7 24.8 24.6 24.8 24.7 24.8 24.4 24.9 .24.3 24.8 24.6 24.7 24.2 pH (SU) 7.88 7.64 7.86 7.63 7.83 7.67 7.97 7.60 7.83 7.61. 7.83 773 7.91 ;. 7.71 86.4% DO (mg /L) 7.9 7.9 7.9 7.4 7.8 7-2 7.8 7.31 7.8 7.3 7.8 7.6 7.8 7.6 Co n d u ctivity o s/c0mtmh

) 15 7 15 7 - 15 5 - 154 16 3 1166 16 8 .

Temperature C'C) 24.9 24.7 24.9 24.6 24.9 24.6 24.8 24.4 24.9 24.4 24.8 24.6 24.8 24.2 pH (SU) 7.85 7.65 7.84 7.63 7.79 7.61 7.88 7.59 7.81 7.60 7.79 7.72 7.89 7.66 DO (mg/L) 8.0 8.0 7.9 73 8.0 7.2 7.8 7.4 7.8 7.2 7.9 7.7 7.9 7.6 Conductivity (gmhos/cm) 128 . 126 .! 128 . 128 133 - 136 137 F 1 0 0% A lkalin ity (m gI L C a CO3 ). 51 .

52 - - . b, 55 5 9-Hardness (mg/L CaCO3 ) 52 - 56 . 58 . * . ... .

Total Residual Chlorine (mg/L) <0.10 q * <0.10 . . . <0.10 7- .

Temperature ('C) 25.0 24.6 25.0 24.6 25.0 24.6 24.9 24.7 25.0 24.4 24.9 24.6 24.8 24.5 p (SU) 7.85 7.71 7.84 7.64 7.80 7.60 7.87 7.63 7.84 7.59 7.81 7.74 7.89 7.73 DO (mnWL) 8.0 8.0 8.0 7.3 8.0 7.0 7.8 7.4 8.0 7.2 8.0 7.6 7.9 7.6 Conductivity (pmhos/cm) 126 - 123 [a 129 127 . 136 136 138 100% Intake Alkalinity (MgfL CaCO 3) 51 . 53 . 56.-5 Hardness (mg/L CaCO3 ) 54 S WIM 56

58 . ,

TotalResidual Chlorine ( rg/L) <0.10 - . <0.10 .* <0.10 -. -.

Temperature i (7C) 1 24.9 i24.6 24.8 24.6 25.0 24.5 24.8 24.4 24.9 24.4 24.8 24.5. 24.8 24.1 File: sqn101_051413chem.xlsx Entered by. J. Sumner Reviewed by: _

F:TS 0

0 0'

Page 1 of 7 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphniadubia Client: Tennessee Valley Authority County: Hamilton Facility: Seuoovah Nuclear Plant Outfall: 101 NPDES #: TN0026450 Project #: Rck&

Dilutionpreparationinformation: Comments:

Dilution prep (%) 10.8 21.6 43.2 86.4 100 Effluent volume (mL) 270 540 1080 2160 2500 Diluent volume (mL) 2230 1960 1420 340 0 Total volume (mL) 2500 2500 2500 2500 2500 Test organism source information: Test information:

Organism age: < 24-hours old Randomizing template color: .

Date and times organisms were born m 6%' 1 b O V b Incubator number and shelf "

between: location:

Culture board: O"(Itl Replicate number: i 2 3 4 5 16 7 9 10 Culture board cup number: I %I L I 1 1 6 1" i Ii.11 YWT batch:

Transfer vessel information: I pH = 1 A..L S.U. Temperature = S. 1C Selenarm batch:

Average transfer volume (mL): . q. ___eenasruatchO____,__

Daily renewal information:

.Day Date Test initiation and feeding, MHSW Sample numbers used Analyst renewal and feeding, or batch used Outfall 101 Intake termination time _

0 CSAl __ _ _ __ CA _

(rao3.Q.o mXoS*.o*

1___ ___.___,_.,___ cs*-'

2 .vlI.3A iIoSl t 0 3 _, -

4__ _____- OCAs- I S s AlS1 L Ž03a~

I-w .

5_____ I 0 k~ I'it-_______

n.h1MAI 1-1is 11 - I~S:A1.oI .AL..

6 dsp ri- 11% P& i I&AS al. 6 1506 ri. of 7I2_____

Controlinformation: Summary of test endpoints:

Control-1 Control-2 Acceptance criteria

%of Male Adults: 07. 070 !<20% 7-day LC-50 t) 1.

%Adults having3rdBroods: 1007. iO7, a 80% NOEC

%Mortality: _0, 010 :520% LOEC ICol.

Mean Offspring/Female: &.J 29. -e offspring/female

>15.0 ChV > I l, J

%CV: -.. 7. "ToJ? <40.0% IC25

IoT.

SOP ATI I - Exhibit AT] 1.2, revision 07-01-12 (age 42 of 113

GETS Species: Ceriodaphnladubla Client: TVA / Sequoyah Nuclear Plant, Outfall 101 Date: 0. A" .'

CONTROL-i Survival andReproductionData Replicate number Day 1 2 3 4 5 6 7 8 9 10 1I Young produced 0 Adult mortalit 23 Youngproduced Adult mortalityU C) I

% L -

C* 01

\,- I,-

o C

%-- I C)

\-i o

3 Young produced c) CL)1C)T IAdult mortalityI*__ -- '-- i . ___ -

S 16 IA LT 4 Young produced jAdult mortality I' - "

-I~

-~

- ~kZL I' __

Final Adult mortality _ I- . ii _- '- i _ _ _

61 5oa Young Youngproduced produced ': 1 . Q *LC)o .** O

Adult mortality I .

7 Young produced %Surviva 1 Repro t ion S %aS Total young produced t s". . j 3 32 . s* lo 30 Final Adult Mortality X for 3r Broods It1.. ~, .-. z:....

1 * '__._

Note: M Adult risity (L - live, D dead),s - split brood(singlebrood split betweentwo days),CO-carry over (offpring carried over with adultduring trasfer).

IConcentration:

%Mortality:

Mean OffsprngFlemale:

CONC; 10.8% Survival andReproduction Data

'-I

- ______ ~Replicate number____

Day 1___ 2,314 1.5 16 1.71819110 I produced dlYoung 01 1 ) 0 0 1 (3 L0 I Adult mortality _ I -" 1....

2 [ Young produced

_fAdult mortalityJ [

[ VU J-t

_ 1 0

[_ 0I L. _

10 3 Youngproduced Cý C.* I".c , *0 a) 0 .

Adult mortality J_ II I L.J.. ..

4 __ Adult mortality Youngp prio uce1l'-I'-I.-

dilt rodd 1ewet C -q-J o o ia c Lo _ i 1C in tr e h 6 Young produced 0 ______ ___I~ ~ IC

_ Adult mortality Adul mortality DE -- 1% I \- I-\-'~

V& 1i %\A .o 11 1S 7]Young Total young produced produced 3' 16 II :M ~310 ' 31 b 13 410 fFinal Adult Mortality Note:, MAdltmortality (L = live,D - dead),

t SH= split brood(single brood split between two days),

.'- -I CO-canovetvr (afltrarin- crained over with adult during transfelr).

Concentration:

% Mortality: 0 j, Mean Offspring/Female: I 2. V

%Reduction from Control-1: ,.'?o (tage 43 of 113 SOP AT 1I - Exhibit AT 11.2, revision 07-01-12

TTS 4'*t".... tsd* ..... '*" Page 3 of 7 Species: Criodaphntaidubla Client: TVA / Sequovah Nuclear Plant, Outfall 101 Date: pS.i',t CONC: 21.6% Survival andReproduction Data S Replicate number Day 1 2 3 4 5 6 17 8 9 10 I Young producded Adult mortality _

0

_ _ I C) _ _

0).

t 0

0 2 Young produced Adult mortality _[ *jI. I .. t '. ____ ' [

I.. -

3 Young produced _ 0 D 0 0 C D Adult mortality 4 1Young produledJi II...LLi5I' J$ a j.

Adult mortality 5 Youngproduced LT l " I %-

V " T Adult mortality .- %__~ -I'-]-I-I- '.

6 Young produced ~0] {0 _ )_ _

7 Young produced I~of S 14 In ' ~ I__

%S.ri Total young produced S-A J* -6% bL ..as 3b

32. 3.- &S -q Final Adult Mortality I- - - \- %.-I %- t ý.. I Note: Adult mortality (L - live, D - dead). SB split brood (single brood split between two days). CO - over (ofifprin carried over with adult during transferl Concentration:

% Mortality:

Jl i

Mean Offspring/Female: j

% Reduction from Control-1: -.

14 CONC: 43.2% Survival andReproduction Data Replicate number Day 1 2 32 4 5 6 7 8 9 10 I Yong produced 0L 11 j 0)C Ault mortal ityd FL~ L-_ I_ L-2 JYoung produced 1 c) c1 1 0 6QT 0 1IC[

1 Adult Adult mortality mortality LL L L L- IO F2-Iý[O-

.I*,O 3 O_*.

3 Yung produced )I~

0C1 10 Z0 0) 0 1 0 4 Young produced Adult mortality _I--,

5 j Young-produced J IS oID t Adult mortality j. \, - %.I _ _ L~I' LI Adult mortality _____ . .U - __

Total young produced a 6 q w. m "A4 3- is 3-1 Final Adult Mortality '- '- '- I - ....-.

Note: Adult mortality (L - lve,D - dead). SB - split brood (sinale brood split between two days). CO cars over (otfbpring cared over with adult during transfer).

Concentration: I

%Mortality: 07.

Mean OffspringfFemale: *,.L

% Reduction from Control-1: - 1*.

- age44 of 113 SOP AT 1I - Exhibit ATI 1.2, revision 07-01-12

'ETS "*"*=*""Page 4 of 7 Species: Cerlodaphniadubia Client: TVA / Seauovah Nuclear Plant. Outfall 101 Date: 0. A. %3b CONw: 86.4% Survival andReproduction Data Replicate number Day -- 1 2 3 ( 4 5 6 7 8 9 10 I

1dl mortality Young produced II Ifi 2 Young produced Adult mortality _

n

) I

- 1 ko) 10 f

_____[r oAcd k* *'! *..n 35 Young mo produced _o oi- 0 10t _L.1* L L.

10101* -. _.**.

C) i .

6 Adultmortality % I %. I. .l_-[ . %-- [.I\ . -" k -"L.

T' I I I '41 41 Young produced JAdult mortality J'f fW '

~.. ~~ ~ C 1~4 1'S TYoung Total Y oung produced I -

Final Adult mortality L... __.'-. '.-. V- -- -. I '-

6 produced L ]01 C 0C LI zIo Io 0

___Adult mortlity I',- I

%_ \ I"- -I'1- -1'1'.

Total young produced 515 541v3%. k VA 5* N 1Final Adult Mortality ~ ,~ j- - ~ ~ 'L-Note: Adult mortality (L live,D dead), SB = split brood (single brood split between two days). CO =ca over (offprlnR carried over with adult durin; transfer).

Concentration:

% Mortality: 07 J Mean Offspring/Female: ,.L,

% Reduction from Control-1: . i..s .

CONC: 100% Survival and ReproductionData Replicate number Day ,, 1 2 3 4 5 6 7 8 9 10 I) n I

I Young produced.

Adult mortality 1-1 0 L.... ýL- I L I %

C) 0 L..

2 *Youngproduced 0 i 0 Adult mortality 3 Young produced 1 _ I 1 11 Adult mortality -_ I L_-K..I.__ k i I.. L.=,..._

4 Young produced SLAumri.., L 'A 2 It.. 'IA Adult mortalityI'I - - LJ - -hL LJ"-IL.

6 Young produced 1 i_.. ]

6 [Young produced J J 0 01 0* t* 1 0 1 Adult mortality \-- k_ 1 L_ k- IL_ ....

71Young produced I( I_Ok V1 'I. 16 V~ V j I % IC I" Total young produced 3S .1 31 .3 IL 3S 3L I3e 3 1 Final Adult Mortality .U 7 L 'a q Z J

U ,;m*outt lltuty %ý.-lie'V , O¢t1. sp i-altro~ou týlslwe tr SlPt e~tWr tWO vw~y,, -, -- c4nl Over Ito lprig car.-Il over .il aUIu~ll dlig tram ~ll,.

Concentration:

Mean Offspring/Femnale: 36.2.

%Reduction from Control- I: I.7 SOP ATI I - Exhibit ATI 1.2, revision 07-01-12 t age 45 of 113

j ETS..... * ....~utn* e Page 5 of 7 Species: Ceriodawhniadubla Client: TVA / Sequoyah Nuclear Plant. Outfall 101 Date: 0%.Il% Us CONTROL-2 Survival and ReproductionData

.Replicate number Da, 1 2 5 6 7 8 9 10 Young produced 01 C .. cI C> Q(. I C)

S Adult mortality I I- I \ - I \ - \- \. t -

2 oung produced 0n 1o C 010 0 0 1_n 5 Young produced Adult mortality [LI 0 0 I_

%-- 0 %-- IL-' o I-ý__

I_ CL_ I__ o 0 I.

Adult mortality 4 Young produced 0 S s 1____SI q HE \ - \-I \1-5oa Adltrtl young produced M I C" to5 10. t t. ek to "1 10 0"L Final Adult Mortality I \ \2 \- *- \- * * - !- =

X for 3rdBroods * ' * < )C . 75* 5 7 '"

Note: Adult mortality (L = li t bit between two day), CO carry over (osprng carried over with adIlt during transfer) .

[ Concentration:

%Mortality I Mmean OffsprinFemale: I CONC: 100% Intake Survival andReproduction Data Replicate number Day 1 2 13 4 5 6 7 8 9 10 I.. ~, 2 Ad u lt m Young o rta lity produced Adult mortality 1 L - .. '.. C \ ..-

K - L -

L - 1 X - ! t.

Ii 3 Young producfed ~.V

~ 0 D0 L.J...J..0.

GC I-I I2I

i. j Adult mortality I--- L L 4 Young produced L . _

Adult mortality 1 CL--. .

5[ Young produced " .1O i I# I I 2. 1

i. I " I __ I

6. Young produced C i 0. 1C I. (D Cl o. . .

___ Adult mortality I'C ",I-- --I IL- - __

It- I\.- L-W.

7 Young produced J~ Q4 IO I'L0 1 -L - I -

Total young produced 3A 3S se .31. 1- a IA S si6 3q Final Adult Mortality ff C -\. -IC- \ Z -- %.- I \-

Note: Adult =orta ity (.L- tivem D - dead), SB - split brood (single brood split between two days). CO - can over (offipring carried over with adult durini transfer).

Concentration:

%Mortality: 7,.

SMean Offspring/Female: 35.9

%Reduction from Control-2: - 21. 37.

SOP ATI1 - Exhibit AT 11.2, revision 07-01-12 46 of 113

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 14-21, 2013 eET AutSI Verification of CeriodaphniaReproduction Totals (D

0 Control-1 86.4%

my_r_ _ Replicate numberToa DayReplicate number Total Dal 1 2 3 4 5 632 7 8 9 1 30 1 T 2 3 4 5 6 7 8 9 10 Tta1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 . 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 5 5 4 3 5 4 L_ 4 4 4 42 4 5 4 5 5 5 5 6 4 4 5 48 5 10 10 13 10 11 10 12 11 11 9 107 5 13 12 14 11 14 12 13 12 13 13 127 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 16 14 14 19 17 16 17 15 17 17 159 7 19 15 15 18 19 19 17 18 15 18 173 Total 31 30 32 31 30 33 3 32 30 30 3 3108 Total 35 34 34 38 36 36 34 32 36 348 10.8% 100%

Re licate number Total "Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 1. 0 02 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 -0 -0 0 0 0 0 0 0 4 5 4 5 4 r4 4 5 4 5 5 45 4 5 5 5 4 6 4 5 6 6 4 50 5 10 12 12 10 12 13 10 12 11 11 112 5 12 13 13 13 14 12 12 14 12 14 129 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 18 18 14 19 17 1 7 16 1 171 7 18 14 18 18 19 13 161 19 19 Total 33 30 31 33 33 33 32 30 33 33 321 Total 7 158 20 19 3 171 31238 36 35 36 3 296 37 3629 21.6% Control-2 Da Re plicate number Total Re Dlicate number D __1_F2 3 14 5 6 7 18 19 110 Day 1 2 Total 3 4 5 6 7 8 9 10 1 0 0 0 10 0 0 0 0 0 -0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 10 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 10 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 5 4 5 4 6 6 4 46 4 6 4 654 5 4 54 5 043

.5 12 12 10 12 12 12 11 13 12 13 119 5 12 13 10 10 10 9 10 12 13 12 104 6 0 0 0 0 0 0 0 0 0 0 O 6 0 0 0 0 0 0 0 0 0 0 0 7 18 15 18 17 19 16 117 15 15 17 17 7 15 15 13 16 2 14 21 18 18 16 149 Total 34 r12 -3457 3 34 33 4 32 Total 31 29 28 361 29 1 27 29 3 29 29 296 43.2% 100% Intake DyRe DyReplicate number Total 1

Ia 2 T37 4 Dficate 5 number 6 17 8 19 101 Total DY1 2 3 4 5 6 17 18_ 9 110 1 0 0 0 0 0 0 0 0 0 0 1 0 1- 0 0 0 "0 0 0 0 0 0 0 0 2 0 0 0 0- 0 0 0 ,0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0- 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 6 4 6 5 5 5- 4 5 5 50 Z4 4 5 6 5 5 4 5 5 5 5 .49 5 12 13 14 12 12 14 12 12 1 2 126.

6 0 0 0 0 0 0 0 0 0 0 0 1 6 [0 0 0 0 70_

0 0 0 0 0 7 19 16 18 18 IS519 D7 17 16 16 17 7 16 1 8 119 19 2 7 21 18 1 7 183 Ttl 3 34 36 33 33 34 35 34 313 34 34 1oa34 135 139 136 137 136 37 35 3 4 359 File" sqnlO1 051413data.xlsx Entered by: J. Sumner Reviewed by-

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 14-21, 2013 (DS Ceriodaphniadubia Chronic Whole Effluent Toxicity Test EPA-821-R-02-013, Method 1002.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Environmental Testing Solutions, Inc.

Project number: 8968 Reolicate number Survival Average reproduction Coefficient of Percent reduction from variation (%) control (%)

(%) (offspring/female) 4 5 6 7 8 9 10 31 30 32 32 30 30 30 100 30.8 3.0 Not applicable 33 33 33 32 30 33 33 100 32.1 4.0 -4.2 34 35 33 32 34 33 34 100 33.2 3.7 -7.8 33 33 34 35 34 33 34 100 34.2 3.3 -11.0 34 38 36 36 34 32 36 100 34.8 6.3 -13.0 34 36 35 36 38 36 37 100 36.2 3.6 -17.5 31 29 27 29 34 29 29 100 29.6 6.6 Not applicable 36 37 36 37 35 36 34 100 35.9 4.2 .21.3 Outfall 101: MSD = Minimum Significant Difference Dunnett's MSD value: 1.439 PMSD = Percent Minimum Significant Difference PMSD: 4.7 PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatnent that can be declared statistically significant in a whole effluent toxicity test.

Intake:

Dunnett's MSD value: 1.359 Lower PMSD bound determined by USEPA (I01h percentile) = 13%.

PMSD: 4.6 Upper PMSD bound determined by USEPA (90"' percentile) = 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH USEPA. 2001a, 2001b. Final Report: Interlaboratoiy Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-82I-B-01-004 and EPA-821-B-01-005.

US Environmental Protection Agency, Cincinnati, OH.

File: sqn101_051413data.xlsx Table populated from associated 'Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Spreadsheet entered by: J. Sumner Reviewed by:

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated IETS 0

o May 14-21, 2013 Statistical Analyses Ceaiodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/14/2013 Test ID: CdFRCR Sample ID: TVA/ SQN 101 End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dlscharge Monitoring Report Sample Date: May2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Cedodaphnla dubla Comments:

Conc-% 1 2 3 4 6 6 7 8 9 10 Control-1 31.000 30.000 32.000 31.000 30.000 32.000 32.000 30.000 30.000 30.000 Control-2 31.000 29.000 28.000 31.000 29.000 27.000 29.000 34.000 29.000 29.000 10.8 33.000 30.000 31.000 33.000 33.000 33.000 32.000 30.000 33.000 33.000 21.6 34.000 31.000 32.000 34.000 35.000 33.000 32.000 34.000 33.000 34.000 43.2 36.000 34.000 36.000 33.000 33.000 34.000 35.000 34.000 33.000 34.000 86.4 37.000 31.000 34.000 34.000 38.000 36.000 36.000 34.000 32.000 36.000 100 35.000 38.000 37.000 34.000 36.000 35.000 36.000 38.000 36.000 37.000 intake 34.000 35.000 39.000 36.000 37.000 36.000 37.000 35.000 36.000 34.000 Transform: Untransformed Rank 1-Tailed Isotonic Conc-% Mean N-Mean Mean MIn Max CV% N Sum Critical Mean N-Mean Control-1 30.800 1.0405 30.800 30.000 32.000 2.984 10

33.550 1.0000 Control-2 29.600 1.0000 29.600 27.000 34.000 6.605 10 10.8 32.100 1.0845 32.100 30.000 33.000 4.008 10 134.50 75.00 33.550 1.0000 21.6 33.200 1.1216 33.200 31.000 35.000 3.703 10 148.00 75.00 33.550 1.0000 43.2 34.200 1.1554 34.200 33.000 36.000 3.320 10 155.00 75.00 33.550 1.0000 86.4 34.800 1.1757 34.800 31.000 38.000 6.325 10 149.50 75.00 33.550 1.0000 100 36.200 1.2230 36.200 34.000 38.000 3.637 10 155.00 75.00 33.550 1.0000 Intake 35.900 1.2128 35.900 34.000 39.000 4.245 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <= 0.01) 1.23325 1.035 -0.3114 0.12786 Bartlett's Test indicates equal variances (p = 0.14) 8.35023 15.0863 The control means are not significantly different (p = 0.10) 1.75662 2.10092 Hypothesis Teat (1-tall, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 100 >100 1 Treatments vs Control-1 Linear Interpolation (200 Resamples)

Point % SD 95% CL . Skew IC05 >100 IC10 >100 IC15 >100 1.0, IC20 >100 0.9e IC25 >100 0.8 IC40 >100 0.7 IC60 >100 0.8 0.5 0r 0.4 L 0.3 0.2

.0.1 0.0 4

-0.1-----------------

-0.2 0 50 100 150 Dose %

Dose-Response Plot 45 40 35 30

25 120 S15 10 5

0 3 04 0 8 8 File: sqnI01.05l4l3dath.xlsx E*tered by: J Sw.er age 49 of 113 Reviewed by

TVA / Sequoyah Nuclear Plant, Outfall 101 Non-treated May 14-21, 2013 0ET Statistical Analyses Statistics used for PMSD calculation only.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5114/2013 Test ID: CdFRCR Sample ID: IVA I SQN 101 End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments:

Conc-% 1 2 3 4 5 6 7 8 9 10 Control-I 31.000 30.000 32.000 31.000 30.000 32.000 32.000 30.000 30.000 30.000 Control-2 31.000 29.000 28.000 31.000 29.000 27.000 29.000 34.000 29.000 29.000 10.8 33.000 30.000 31.000 33.000 33.000 33.000 32.000 30.000 33.000 33.000 21.6 34.000 31.000 32.000 34.000 35.000 33.000 32.000 34.000 33.000 34.000 43.2 36.000 34.000 36.000 33.000 33.000 34.000 35.000 34.000 33.000 34.000 86.4 37.000 31.000 34.000 34.000 38.000 36.000 36.000 34.000 32.000 36.000 100 35.000 38.000 37.000 34.000 36,000 35.000 36.000 38.000 36.000 37.000 Intake 34.000 35.000 39.000 36.000 37.000 36.000 37.000 35.000 36.000 34.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-I 30.800 1.0405 30.800 30.000 32.000 2.984 10 Control-2 29.600 1.0000 29.600 27.000 34.000 6.605 10 10.8 32.100 1.0845 32.100 30.000 33.000 4.008 10 -2.066 2.287 1.439 21.6 33.200 1.1216 33.200 31.000 35.000 3.703 10 -3.814 2.287 1.439 43.2 34.200 1.1554 34.200 33.000 36.000 3.320 10 -5.403 2.287 1,439 86.4 34.800 1.1757 34.800 31.000 38.000 6.325 10 -6.357 2.287 1.439 100 36.200 1.2230 36.200 34.000 38.000 3.637 10 -8.582 2.287 1.439 Intake 35.900 1.2128 35.900 34.000 39.000 4.245 10 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates non-normal distribution (p <- 0.01) 1.23325 1.035 -0.3114 0.12786 Bartlett's Test indicates equal variances (p = 0.14) 8.35023 15.0863 The control means are not significantly different (p = 0.10) 1.75662 2.10092 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnetts Test 100 >100 1 1.43883 0.04672 37.59 1.97963 7.5E-11 5,54 Treatments vs Control-I File: sqn]01 051413data.xlsx Entered by: J. Suniper Reviewed by:::

tage 50 of113

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake Non-treated May 14-21, 2013

'ETS Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/14/2013 Test ID: CdFRCR Sample ID: TVA/SQN 101 - Intake End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Dischsrge Monitoring Report Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubia Comments:

Conc-% 1 2 3 4 5 6 7 8 9 10 Control-I 31.000 30.000 32.000 31.000 30.000 32.000 32.000 30.000 30.000 30.000 Control-2 31.000 29.000 28.000 31.000 29.000 27.000 29.000 34.000 29.000 29.000 10.8 33.000 30.000 31.000 33.000 33.000 33.000 32.000 30.000 33.000 33.000 21.6 34.000 31.000 32.000 34.000 35.000 33.000 32.000 34.000 33.000 34.000 43.2 36.000 34.000 36.000 33.000 33.000 34.000 35.000 34.000 33.000 34.000 86.4 37.000 31.000 34.000 34.000 38.000 36.000 36.000 34.000 32.000 36.000 100 35.000 38.000 37.000 34.000 36.000 35.000 36.000 38.000 36.000 37.000 Intake 34.000 35.000 39.000 36.000 37.000 36.000 37.000 35.000 36.000 34.000 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Control-1 30.800 1.0405 30.800 30.000 32.000 2.984 10 Control-2 29.600 1.0000 29.600 27.000 34.000 6.605 10 10.8 32.100 1.0845 32.100 30.000 33.000 4.008 10 21.6 33.200 1.1216 33.200 31.000 35.000 3.703 10 43.2 34.200 1.1554 34.200 33.000 36.000 3.320. 10 86.4 34.800 1.1757 34.800 31.000 38.000 6.325 10 100 36.200 1.2230 36.200 34.000 38.000 3.637 10 Intake 35.900 1.2128 35.900 34.000 39.000 4.245 10 -8.037 1.734 1.359 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92329 0.868 1.00343 1.21965 F-Test indicates equal variances (p = 0.47) 1.64593 6.54109 The control means are not significantly different (p = 0.10) 1.75662 2.10092 Hypothesis Test (1-taill, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 1.35927 0.04592 198.45 3.07222 2.3E-07 1, 18 Treatments vs Control-2 Dose-Response Plot 40 35.

30

. . . . . . .. . . . . . .. . -.. 1-tail, 0.05 level of significance 25 0

L- 20:

10 N C2 V CO 8

File: sqn 101 _051413data.xlsx Entered by: I. Skumer Reviewed by: T 7 I age 51 of 113

ETS *'+*"**'*'*Page e 6 of 7 Species: Ceriodaphniadubia Date: Aa-.t4-.

Client: TVA / Seauoyah Nuclear Plant, Outfall 101 Daily Chemistry:

Day (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.)

I I . 1 1 2-Analyst 1 ý 0 1- et 11 - Ir I V.1m 11 -r-*ft w

Concentration Parameter Concentra io pH (S.U.)

DO (mg/L)

Conductivity (umhos/emn) I-CONTROL *Alkalinity (mp-, CaMIL) I -

Hardness (me CaCOUL)

-I --

Temperature (IC) 10.8%

PH fDO p(S.U.)

(mg/L)

Conductivity II

___________ Temperature C0C) F%~ 0 DH (S.U.)

9 -*~==------II--

DO (maL) 21.6% Conductivity (Mmhos/cm)

Temnerature P°CI

- - 1- 1! I - , ' i - - Iý i -. 11 - II--

pH A

.S.U.) 7.5 7. 7.10 7l DO (mg/L) 7qT 43.2% Conductivity imhos/cm) ?,4Q VC .31 pH (S.U.) 7.19 ..7.Sarc. 7.17.

DO (mg/L) 86.4% Conductivity 17 (pimhos/cm)1517

__________ *Temperature (0Q) -MA. .

PH (s.u.) 7.11S 757.5 14 7.7 DO (mg/Q . .1 9 Conductivity

(*mos/em) i 2 z 100% *Alkalinity (mg CaCO31L)

Hardness (mg CaCO3/L) S

TR chlorine (mg/L) 4 .t0.

Temperature (%A.-& - " Q ' . "S.. .

pH (S.U.) w s7 DO (mgIL)

Conductivity CIgmhos/cm) 100% *Alkalinity Intake (mg CaCO3/L)si

Hardness (mg CaCO 3IL) -I

TR chlorine (mg/L) . 0

________ *Temperature (OC IS.IL. A, . .18 .

Initial Final Initial Final Initial Final

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal n&p~nd~~~

Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:

SOP ATI I - Exhibit ATI 1.2, revision 07-01-12 age 52 of 113

PETS Page 7 of 7 Species: Cerlodaphniadubia Client: TVA / SeQuovah Nuclear Plant, Outfall 101 Date: ~~s~*'4*t~

Dae mstI0 Day II (Analyst identified for each day, performed pH, D.O. and conductivity measurements only.) Ii Analyst I-Concen- Parameter tration I pH (S.U.)

DO (mg/L) *wJ L Conductivity (umhos/cm) I.

CONTROL *Alkalinity (menCaCOVUL I- -~----

Hardness (miz CaCOIL)

Temperature (0Q

-- p * -

BH (S.U.')

p .- -~----

74 7 II -7AaJ 4..............I 1

I1 740 V-

'7-q7 7V I

-Lq~c .a ~ J 10.8%

DO (mP/L)

Conductivity

Temperature (QC)

I__ 7.7 7.7 .7.9 7.7 Z~5 1.Ir I "7.'/ "is "LS.O -LA V4,.1 "t.I. "iS-I U U ~ . - - 0 4 0 r

DH (S.U.) 7 Ig 1.1-. q I 7y.90 7.R9l 7,qC 79C 7^I-4 21.6%

DO (meIL' Conductivity (gmhos/cm) i _Z4' II~

IT/L

Temverature (C) 'iA.O ~ V4 kM PH (S.U.) 77.40L DOm 7.9 71 43.2% Coinductivity I_3 S

Temperlture (*C) i' 4 DH (S.U.)

. . .. I 2qM 11 '7A'1

.. - = =

I i I D= u* II DO (mg/L) 1. - 7.9 7 7.9 .

86.4% Conductivity (gimbos/cm)

Temperature (*C)

U U-ni (S.U.) 0. tOrs 7A. 7.

DO (mg*L)

Conductivity

([imbos/cm)

Alkalinity 100% (mg CaCO3IL)

Hardness (Ing CaCO3/L)

~~~.19 . ~ o 1

TR chlorine (mg/L)

Temoerature ( C' 0 INl 133 1

pH (Sm.)

DO (m~g/L)

Conductivity

]

6mhos/cm)

J 100%

Intake

Alkalinity

Hardness I

I s~ip

TfR chlorine (mg/L)

Temperature (0C)

- I

-T Finl til inl Iital 11 Final

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Iinitial rFinal Information table located on Page 1. Alkalinity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: JA _ .

e 53 of 113 SOP ATI1 -Exhibit AT1 1.2, revision 07-01-12

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treated May 14-21, 2013 Ceriodaphniadubia Chronic Whole Effluent Toxicity Test 0 EPA-821-R-02-013, Method 1002.0 0 40 E .T. S , Daily Chemical Analyses Environmental Testing Solutions, Inc. Project number. 8968 Concentration Parameter Da 0 Day 1 Day 2 Day 3 Da 4 Day 5 Da 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final pH (SU) 7.93 7.89 7.93 7.94 7.97 7.95 7.97 7.93 7.89 7.96 8.00 7.95 7.93 7.87 DO (mWL) 7.7 7.9 7.8 8.0 7.7 7.8 7.7 7.8 7.6 7.8 7.7 7.8 7.7 7.8 Conductivity (prohos/cm) 313 . 309 307 I 310 308 311 314 Control Alkalinity (mg/L CaCO 3) 61 . 62 . . 61 61 Hardness (mg/L CaCO3 ) 84 . 8888 88 88 Temperature OC) 24.9 25.1 24.8 25.2 24.8 25.1 24.9 24.8 24.8 25.0 24.7 24.9 24.7 25.1 PH (SU) 7.96 7.89 7.93 7.95 7.95 7.96 7.97 7.94 7.90 7.98 7.97 7.96 7.94 7.88 DO (MRIL) 7.9 7.9 7.8 8.0 7.8 7.8 7.7 7.8 7.7 7.8 7.7 7.8 7.7 7.8 10.8% Conductivity (Pmhos/cm) 290 . 2859 287 . 283 , 286 . 284 285 Temperature (7C) 25.0 24.9 24.9 25.0 24.8 24.8 25.0 25.0 24.8 24.9 24.7 25.1 24.7 25.3 PH (SU) 7.97 *7.88 7.93 7.94 7.95 7.96 7.98 7.94 7.90 7.99 7.96 7.96 7.94 7.89 DO (m_/L) 7.9 7.9 7.8 8.0 7.8 7.8 7.8 7.9 7.7 7.8 7.7 7.8 7.7 7.8 Conductivity (amhos/cm) 272 . 270 s 268 v 267 . 269 . 270 . 271 O _&

Temperature (CC) 25.0 24.9 24.9 25.0 24.8 24.8 25.0 25.0 24.8 24.9 24.7 25.1 24.7 25.0 PH (SU) 7.96 7.88 7.92 7.94 7.90 7.97 7.98 7.94 7.90 8.00 7.90 7.97 7.94 7.90 DO (mg/L) 7.9 8.0 7.8 8.0 7.8 7.9 7.8 7.9 7.8 7.9 7.7 7.8 7.7 7.8 43.2% Conductivity (Ounbos/cm) 234 236 231 232 . 235 . _237_ 235

Temperature CC) 25.0 24.9 25.0 24.7 24.9 24.8 25.0 25.0 24.8 24.9 24.7 24.9 24.8 25.0 PH (M 7.88 7.83 7.86 7.90 7.83 7.97 7.97 7.94 7.83 7.97 7.83 7.97 7.91 7.87 DO (mg/L) 7.9 8.0 7.9 8.0 7.8 7.9 7.8 7.9 7.8 7.9 7.8 7.8 7.8 7.8 86.4% Conductivity (pmhos/cm) 157, _ 157 155 154 163 : 166 .* 168 .IOt*

Temperature C°C) 25.1 25.0 25.0 24.9 24.9 24.8 25.0 25.1 24.8 25.1 24.8 24.9 24.8 24.9 PH (SU) 7.85 7.83 7.84 7.88 7.79 7.93 7.88 7.90 7.81 7.98 7.79 7.94 7.89 7.87 DO (mg/L) . 8.0 8.0 7.9 8.0 8.0 7.9 7.8 8.0 7.8 7.9 7.9 7.9 7.9 7.8 Conductivity (pmhos/em) 128 126n w 128 . 128. 133 136 137 100% Alkalinity (mg/L CaC03) 51 52 55.

lHardness (mg/L CaCO 3) 52 . 56 .58 Total Residual Chlorine (ma/L) <0.10 .. - <0.10 . <0.10 . . -.

Temperature (*C) 25.3 24.8 25.0 24.9 25.2 24.9 25.0 24.8 25.0 25.1 24.8 24.9 24.8 24.9 pH (SU) 7.85 7.84 7.84 7.81 7.80 7.93 7.87 7.91 7.84 7.97 7.81 7.93 7.89 7.86 DO (mg/L) 8.0 8.0 8.0 U.1 8.0 7 8.0 8.01 8.0 7.9 7.9 7.9 Conductivity (pmhos/cm) 126 123 129 12137 136.136 138 100% Intake Alkalinity (mg/L CaCO3 ) 51 53 56 Hardness (mg/L CaCO3) 54 5656.8.

Total Residual Chlorine (ml/L) <0.10 . <D.10. . . <0.10 . .

ITemperature (°C) 25.2 24.9 24.9 25.0 24.9 24.9 25.1 24.8 24.9 24.8 24.9 25.0 25.0 24.9 File: sqn101_051413chem.xlsx Entered by: J. Sumner Reviewed by: k

Page 1 of 6 Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas Client: Tennessee Valley Authority County: R-ao 14Nv LrOt Facility: Senuo ah Nuclear Plant Outfall: 101 NPrESt#: P & 9 -rKat 6 0 Project #: e -kt Dilutionpreparationinformation: Comments:

Dilution prep (%) 10.8 21.6 43.2 86.4 100 Each concentration was UV-treated Effluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenic Diluent volume (mL) 2230 1960 1420 340 0 Interferences.

Total volume (mL) 2500 2500 2500 2500 2500 Test organism information: Test information:_

Organism age: 'U.S. AWA&$ ck-t Randomizing template: '&_LAC_

Date and times organisms 05-15-1 IlCo) Incubator number and 56 were born between: shelf location: _

Organism source: Any. 60TPUA 0a. 1& 13 A remia CHM number:. CMI Drying informationfor weight determination:

Transfer bowl information: pH = .16 S.U. Date / Time in oven: oi5 ?.%# %I Temperature = "Lq.'I 0C Initial oven temperature: ý0(0(

Average transfer volume: . Date/ Time out of oven: C&-o-L.%%

Final oven temperature: 6WLat.

Total drying time: " *q Dailyfeeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, Sample numbers used MHSW renewal, or batch termination used Time Analyst Time Analyst Time Analyst Outfall 101 Intake 0 a____-14_ *s_ 1S S l . V O --- , t! o$I"&.O2- OS'-I-I$

2 4S- 11010% OClSO I?" lqtSO yl rV,.'0 V1 110SIS.0 o"1% -, .0 CF i*.lj'tA

__ OQt4.3 j)rbS3 44.

iAK*

%%s

.4*S-b

.J2*

vz 4{. t*~a.'t b tb 0 t41 0$.e1'a3A 6 O .2- IA W1 'ThoIa M Ar, 11 . (n jaos1. O0 c*.-GA I 7

mlbý 1.3 01 U

I Control information: Acceptance criteria Summary of test endpoints:

% Mortality: on. 20% 7-day LC5 o ) 10t7/

Average weight per initial larvae: 0i g R EIRI NOEC t ol, Average weight per surviving larvae: . 0 0.25rglarvae LOEC 4 7ob0 .

ChV >t007.

IC25 . I >.t007.

SOP AT20 - Exhibit AT20.3, revision 07-01-12 (tage 55 of 113

Page 2 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date: OS - %+a Survival and Growth Data Day CONTROL 10.8% 21.6%

A B C D E F G H I J K L 0 )oo I I IC) 1 10 to toIC S, K) ,o o to to,~to ,o113 to C)I

,o ,o'o 'o (0 o /.0 ,o 210 10 10 (01 10 (0 0- LO 10 10 10 3

4_ _0 tO Q) iD l0 i0 10 (0 to IC0/0 )

4 ID 10 (0'0. 10 (0IC) tO/0 /0tO 5 I tolt),to it)

,o I 1o r0to II0) 6 ,~10 1C) 1 1( 10 '() 10 10)1 10 10 to 7 Lo 10 10 to 10 0/0 1to0 A = Pan weight (nag)

Tray color code:: Qrs gterl',,w q5 Analyst:

Date:

t2378,3.

o5.oq.13 1. j27 1378 I.1__ J2.. 1__. 1 . .10 15515S0 177CM'I~ 1188 i.; 8I.Xv, O tc I737 B = Pan + Larvae weight (mg)

Analyst:

Date: astZZ'0 B- A 117 1_.7_ _

S1

___ . I1.6O C = Larvae weight (mag) =

Hand calculated. S.0b SA& AS S S I*Jk 4-V*S 61

1 1P..-.. *S.'.

Analyst:

Weight per initial number of larvae (mg)

= C / Initial number of larvae Hand calculated.

Analyst:

8. elk CI, '2

0. ts.

~0 IV 0'

- I - *- I - S - S i - h Average weight per Percent reduction 6..&1.

initial number of larvae (mg) from control (%)

I 0 .Si

d. - I

- t..10.

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk sick, sm= unusually small, Ig = unusually large, d&r decanted and returned, w = wounded.

]

Comments:

-age g 5oSOP 56 of 113 AT2O - Exhibit AT2O.3, rev 'ision 07-01-12

Page 3 of 6 Species: Pimephalespromelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date: .

Survival and Growth Data Day 43.2% 86.4% 100%

M N 0 P Q R 8 T U V W X 0 to 10 10 .10 1() IO(0 10 10 1 10 to 10O

_____ IQ 10 10 10 to to It) 10 10 /0 i0 2 1

() I0 o ( C (0 to 1 t0 t) 10 10 /o 3 C) IO IO too I* /0o /0 1( 0 1/0 1() 1O 4 __ _ _1 0 1 toQ1¢0 0 0 /0 to .to 1/0 /0 5 1r%)I10 1 10 10 G t 0 1010 10 /01 0 6 D 1010 10. 0 10(0 to to 1 0/Q 1O 7 j o to to toc C to '0 tO 10 to to A - Pan weight (mg)

Tray color code::

Analyst:

Date:

Fane,4 gree lyst,

_q_0_1_7_135__.

o$.__ q. 13__

_ q C1q.57 j?.8? q 11.7q 13.q( 13.3g B - Pan + Larvae weight (mg)

Analyst:

Date:

. T' o,.fl..,

C fiLarvae weight (rag) =B - A 1__.1___.7 .L/q*= __

q "2 I&a1*Z 1i. 718519331.51 72 I r r7 1'711 Hand calculated. SAS .6.11& $.1*j S.4q ,' L . 1, isL 0Stv

. s.,-i &.4La .

so i6sa Analyst:

Weight per initial number of larvae (nmg)

= C /Initial number of larvae Hand calculated., ,___ 64.V; Analyst: o *

Average weight per Percent reduction initial number of from control (%) . O, i.XS - (.'4 O. A'i - -IA 7.

larvae (mg) .,

Comment codes: c = clear, d = dead, fg fungus, k - killed, m = missing, sk = sick, sm = unusually small, ig = unusually large, d&r = decanted and returned, w = wounded.

Comments.

SOP AT20 - Exhibit AT20.3, revision 07-01-12 (tage 57 of 113

Page 4 Of 6 Species: Pimephales promelas Client: TVA / Sequoyah Nuclear Plant, Outfall 101, UV-treated Date: . Oc,,IkA- V%,

Day 100% Intake Y Z AA BB 0 0 to0 o 0'0 10. 1C I0 2 to 1D to (0 3 _10 (

1(0 10) 4 1'0 IC) 10 5.o /0 to (0 6 (I -113 7 /

It 10 '0 to)

A = Pan weight (mg)

Tray color code:: -Fore f ra1n ....

Analyst: %I Ij*'

Date: *05.0-13 .?.2I B.23 13.3..

B = Pan + Larvae weight (mg)

Analyst: 206OG["

Date: O£tzZ- 13* 118%

U193 17 C = Larvae weight (mg) = B - A Hand calculated. ' t, S Analyst:

1..-..'

Weight per Initial number of larvae (mg)

=C /Initial number of larvae Hand calculated. NP Analyst:

Average weight per Percent reduction initial number of from control (%) o},,* s ,,

larvae (mg) -- _____._ _--

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig= unusually large, d&r = decanted and returned, w = wounded.

SOP AT20 -Exhibit AT20.3, revision 07-01-12 (iage 58 of 113

TVA / Sequoyah Nuclear Plant, Outfall 101 U V-treated May 14-21,2013 CD Pimephalespromelas Chronic Whole Effluent Toxicity Test 0 E EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses 4~Environmental Testing Solutions, Inc. Project number. 8968 Not for Compliance Assessment, Internal Laboratory QC Counant-afle(t) Reploate e lainmbero" Fhtu.I-ueheoflasvne -- A-Pan..nt Punn (u) Luevue" tarrae.slobt(-S) Weigbt/Survnldg Meams-wtgbt Ceela-utuouvarlan Welaht/Intut unumber Moan.. rlsut Mean glplhtIltial Coditlntef Percent reducion frnom Larent wlh(ro) -AA-B " numberofla-mae(mg) SorrhiugnumberdoffP."n ol.fher(.W) (%) nuurneoflarvat varkatlan t ,,.rt toutrl (%)

la[-n (ru) ,u n.,-,* %) (mg) n r A 10 10 13.78 19.71 5.93 0.593 0.593 B 10 10 14.27 19.75 5.48 0.548 0.57 3.7 0.548 1ft. 0 3N a b C 10 t0 13.45 19.20 5.75 0.575 0.575 D 10 10 13.78 19.72 5.94 0.594 0.594 E 10 10 12.40 17.99 5.59 0.559 0.559 10.8% GF 10 10 10 10 12.29 12.62 17.88 18.05 5.59 5.43 0.559 0.543 0.543 4.2 0.559 0.543 100.0 0.543 4.2 6.0 H 10 10 14.59 19.70 5.11 0.511 0.511 1 10 10 11.90 17.65 5.75 0.575 0.575

_." l_O 10 13.55 19.39 5.84 0.584 0.592 3.1 0.684 K 10 10 13.47 19.65 6.18 0.618 0.618 L 10 10 13.68 19.60 5.92 0.592 0.592 M 10 10 12.94 18.19 5.25 0.525 0.525 43.2% N 10 10 14.05 19.78 5.73 0.573 0.573 O 10 10 14.71 20.49 5.78 0.578 0.578.

P 10 10 13.58 19.02 5.44 0.544 0.544 Q I0 10 12.90 18.22 5.32 0.532 . 0.532 8&4% R 10 10 12.96 18.92 5.96 0.596 0.615 17-3 0-596 100.0 0.615 12.3 -6.4 S 10 10 14.57 21.72 7.15 0.715 0.715 T 10 10 12.82 18.97 6.15 0.615 0.615 U 10 10 14.06 19.85 5.79 0.579 . 0.579 100% V 10 10 12.74 18.33 5.59 0.559 0.594 5.4 0.559 100.0 0.594 5.4 -2.9 W 10 10 13.46 19.51 6.05 0.605 0.605 X 10 10 13.38 19.71 6.33 0.633 0.633 Y 10 10 13.23 18.46 5.23 0.523 0.523 100%Zntake Z 10 10 12.24 18.31 6.07 0.607 .70.607 100.0 0.545 9.7 5.6 1 AA 10 10 13.3 18.96 5.65 0.565 0.545 9.7 0.565 BB 10 10 13.89 18.74 1 4.85 0.485 0.485 Outfull 101: MSD = Minimum Significant Difference Dounnet's MSD value: 0.0650 PMSD = Percent Minimum Significant Difference PMSD: 11.3 PMSD isanmeasure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent todicity test.

Osstake:

IDunnett's MSD value: 0.053 Lower PMSD bound detemined by USEPA (10th percentile) = 12%.

PMSD: 9.6 Upper PMSD bound determined by USEPA (90th percentile) = 30%.

200 Lower and upper PMSD bounds were determined from the IOthand 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 1a; USEPA, 200 1b).

USEPA. 2001a, 2001b. Final Report. Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole EffluentToxicity Test Methods, Volumes I end 2-Appendix EPA-821-B-01 -004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH File: sqn101 051413data-uvadasx Entered by: J. Supner Reviewed by:.

TVA / Sequoyah Nuclear Plant, Outfall 101 UV-treated May 14-21, 2013 SET Statistical Analyses nuSsn altw n Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/14/2013 Test ID: PpFRCR Sample ID: TVAI SQN 101 End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.6930 0.5480 0.6750 0.5940 10.8 0.5590 0.5590 0.5430 0.5110 21.6 0.5750 0.5840 0.6180 0.5920 43.2 0.5250 0.5730 0.5780 0.5440 86.4 0.5320 0,5960 0.7150 0.6150 100 0.5790 0.5590 0.6050 0.6330 Intake 0.5230 0.6070 0.5650 0.4850 Transform: Untransformed 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean D-Control 0.5775 1.0000 0.5775 0.5480 0.5940 3.726 4 0.5794 1.0000 10.8 0.5430 0.9403 0.5430 0.5110 0.5590 4.167 4 1.280 2.410 0.0650 0.5794 1.0000 21.6 0.5923 1.0255 0.5923 0.5750 0.6180 3.127 4 -0.547 2.410 0.0650 0.5794 1.0000 43.2 0.5550 0.9610 0.5550 0.5250 0.5780 4.503 4 0.835 2.410 0.0650 0.5794 1.0000 86.4 0.6145 1.0641 0.6145 0.5320 0.7150 12.340 4 -1.372 2.410 0.0650 0.5794 1.0000 100 0.5940 1.0286 0.5940 0.5590 0.6330 5.405 4 -0.612 2.410 0.0650 0.5794 1.0000 Intake 0.5450 0.9437 0.5450 0.4850 0.6070 9.667 4 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.92439 0.884 0.51985 3.37587 Bartlett's Test indicates equal variances (p = 0.10) 9.15189 15.0663 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 100 >100 1 0.06498 0.11251 0.00283 0.00145 0.1365 5,16 Treatments vs 0-Control Linear Interpolation (200 Resamples)

Point % SD 95% CL(Exp) Skew ICO5 >100 IC10 >100 IC15 >100 1.0 IC20 >100 0.9 IC25 >100 0.8 IC40 >100 IC50 >100 0.7 0.5 0.0.4 0.3 0.2 0.1 0.0

-0.1~

-0.2 0 50 .100 150 Dose %

Dose-Response Plot 0.7, 0.6 O0.5 I- ..... . ...... 1'_ý_

................ 1-tail, 0.05 level of significance 0.4.

90.3 0.2 0.1 0 cL oi 1 (

c 8: i 8 N -0 .

File: sqnl01 0514l3data-uv.xlsx Entered by: J SFner Reviewed by:

60 of 113

TVA / Sequoyah Nuclear Plant, Outfall 101 - Intake UV-treated May 14-21, 2013 ETS

~Environmental Testing Solutions.Inc.

Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/1412013 Test ID: PpFRCR Sample ID: TVA/SQN 101 - Intake End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring Report Sample Date: May 2013 Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas Comments: UV-treated Conc-% 1 2 3 4 D-Control 0.5930 0.5480 0.5750 0.5940 10.8 0.5590 0.5590 0.5430 0.5110 21.6 0.5750 0.5840 0.6180 0.5920 43.2 0.5250 0.5730 0.5780 0.5440 86.4 0.5320 0.5960 0.7150 0.6150 100 0.5790 0.5590 0.6050 0.6330 Intake 0.5230 0.6070 0.5650 0.4850 Transform: Untransformed 1-Tailed Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 0.5775 1.0000 0.5775 .0.5480 0.5940 3.726 4 10.8 0.5430 0.9403 0.5430 0.5110 0.5590 4.167 4 21.6 0.5923 1.0255 0.5923 0.5750 0.6180 3.*127 4 43.2 0.5550 0.9610 0.5550 0.5250 0.5780 4.503 4 86.4 0.6145 1.0641 0.6145 0.5320 0.7150 12.340 4 100 0.5940 1.0286 0.5940 0.5590 0.6330 5.405 4 Intake 0.5450 0.9437 0.5450 0.4850 0.6070 9.667 4 1.142 1.943 0.0553 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97263 0.749 0.00813 0.26344 F-Test indicates equal variances (p = 0.18) 5.99568 47.4683 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.0553 0.09575 0.00211 0.00162 0.29693 1, 6 Treatments vs D-Control Dose-Response Plot 0.8 0.7 0.6 1-tail, 0.05 level 0.5 of significance 0.4 00.3 0.2 0.1 0'

8 6

File: sqnI0l_05141 3data-uv.xlsx Entered by: J. Siknner Reviewed by:

Page 61 of 113

S ~Page 5of 6 Species: PbmevhalesPromelas Date: 0*.A' Client: TVA / Seauovah Nuclear plant, Outfall 101. UV-treated

=

Daily =

Chemistry: . - .... .

.... . m . .

1 Day (Analyst identified for each day, pebrored DH, D.O. and conductivity measurements only.)

Analyst

d , I Concentration I Parameter SI PH (S.U.)

]DO (mg/L)

Conductivity CONTROL (Ikmhos/cm)

I UV-treated (ing CaC 3/L) -4

Hardness (mg CaCOJ/L) -4

Temnerature (MCl DO(mL) ig7 10.8% Conductivity z.q

."* ,Temperature ()

pH (S.U.) 1.q 7 t278 DO (mngIL) %0 21.6% Conductivity 27

{ (Mmhos/cm) 4 .. 72 7.7C

,_, *Temperature (10) A el pH (S.U.) T7IC _VA4 DO mgL

.1%3 43.2% Conductivity l 7 (gmhos/ 0 7

(*Temperature CC) LAA pH (S.U.) . 7.qO DO (mg1L) 86.4% Conductivity

)- (Aimhos/cm) 17

_____________*Temperature (*C) i Conductivity 1Z77

(~Nihos/crn) 100% *Alkalinity Intaket

Hardness (Mg CaCO3/L)

TR chlorine (mg/L) 0O F10

Temperature (°C) -L,6 X .

DO mgL Conductivity fjnimhoslacm)IT u

100% *Alkejjnjty Intake mg COCOA) S

Hardness

Tfl chlorine (mg/L) 4 0.10

'Temnperature 0 I __________

( QC I IS* IL I Initial Final I initial 0 Final I Initial 11 Final i

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.

nity, hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

hI1Wy L

SOP AT20 -Exhibit AT20.3, revision 07-01-12 Page 62 of 113

Page 6 of 6 Species: Pimephales promelas Client: TVA / Seauovah Nuclear Plant. Outfall 101. UV-treated Date: OL. tA" 130 I

I m

Day (Analyst identified for each day, performed pH. D.O. and conductivity measurements only.) . I Analyst k Concen- Parameter tration I PH (S.U.)

DO (mg/L)

Conductivity (itmhos/cm)

CONTROL *AIlklinity UV-treated (nig CaC0sJL)

Hardness (rag CaCO3/L)

Temnerature (C) g .I 11- -- 41 U - - i IIU -- - - - -

DH (S.U.)

DO (mg/L) 10.8% Conductivity I (umhos/cm) i i

.i *Temperature (MC)

-U TT \

M DO (m-/L) 21.6% Conductivity (umhos/cm)

Temperature (C)

DH (S.U.)

DO (ma/L) 43.2% Conductivity (grmhoslcm)

TemperatureCC pH (S.U.) 7.0 DO (maLL) 86.4% Conductivity (umhos/cm) 1C7

Temperature (0C)

-7. S nD (S.U.)

DO (ma/.)

Conductivity (gmhos/cm)

Alkalinity J' 1* -1.

100% (Ing CaCO 3/L)

Hardness (mg CaCO 3/L)

TR chlorine (mg/L) "-'q1-- "0..

Temperature ( 0C) pH (s.u.)

DO (ma/L)

Conductivity 100% *Alkalinity I Intake

Hardness

TR chlorine (mgiL)

Temperature ( 0 C)

Initial 11 Final I Initial 11 Final I Initial B Final I Initial 11 Final

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.

hardness and total residual chlorine performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet

,ity Total residual chlorine was perfbrmed on non-treated Outfall 101 and Intake samples. " -

, ag SOP AT2e- Exhibit AT20.3, revision 07-01-12

".--Page 63 of113 ".

TVA / Sequoyah Nuclear Plant, Outfall 101 - UV-treated May 14-21, 2013 E) rPimephales promelas Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0 E .. ..... Daily Chemical Analyses Environmental Testing Solutions. Inc. Project number: 8968 w

Concentration Parameter Da 0 Da I Day 2 Day 3 D 4 Da 5 Day 6 Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final Initial Final PH (SU) 7.96 7.81 7.92 7.72 7.94 7.69 7.95 7.67 7.89 7.69 7.94 7.77 7.95 7.69 DO (mg/L) 7.8 7.8 7.8 7.4 7.8 7.2 7.7 7.3 7.7 7.4 7.7 7.4 7.8 7.3 Conductivity (Mmhos/cm) 311 310 - . 303 3036 302 301 306 Control Alkalinity (mg1L CaCO3 ) 61 61 62 62 . ...

Temperature

_ _C_ 24.8 24.7 24.7 24.6 24.9 24.5 24.9 24.6 24.8 24.6 24.7 24.6 24.9 24.7 PH (SU) 7.97 7.80 7.93 7.73 7.95 7.68 7.97 7.70 7.90 768 7.92 7.77 7.96 7.70 DO (mg/L) 8.0 7.9 8.0 7.4 7.9 7.3 7.8 7.3 7.8 7.4 7.8 7.4 7.8 7.3 10.8% Conductivity (tmhos/cm) 294 , 288 C _9 287 _ 281. 284 289 289 -

Temperature (°C0 24.9 24.6 24.8 24.7 25.0 24.5 24.9 24.7 24.8 24.3 24.8 24.4 25.0 24.7 PH (SU) 7.97 7.73 7.93 7.75 7.93 7.69 7.97 7.68 7.90 7.67 7.89 7.76 7.96 7.72 DO (mg/L) 8.0 7.9 8.0 7.4 7.9 7.2 7.8 7.3 7.8 7.4 7.9 7.4 7.9 7.4 Conductivity (pmhos/cm) 274 -. , 272 269 l 4,270 269 274 274, Temperature (C) 24.9 24.6 24.9 24.6 25.0 24.5 25.0 24.5 24.8 24.3 24.8 24.4 25.0 24.8 PH (SU) 7.96 7.73 7.92 7.65 7.93 7.57 7.97 7.68 7.89 7.63 7.85 7.76 7.94 7.77 DO (mg/L) 8.0 7.9 8.0 7.4 8.0 7.2 7.9 7.3 7.9 7.3 7.9 7.4 7.9 7.4 43.2% Conductivity (pnhos/cm) 237 _ _234 _ 2L3 '_232 -232 -237 239 Temperature (C) 24.9 24:4 24.9 24.6 25.0 24.7 25.0 24.5 24.8 24.4 24.8 24.4 25.0 24.6 pH (SM_ 7.90 7.68 7.92 7.64 7.84 7.55 7.96 7.62 7.81 7.54 7.79 7.72 7.90 7.69 DO (ragL) 8.0 7.9 8.0 7.3 8.0 7.1 7.9 7.3 7.9 7.3 7.9 7.4 8.0 7.5 86.4% Conductivity (pmhos/cm) ___ 157 -. W_ _158_ 156 156 _ 164 166 -167

Temperature (OQ 24.9 24.7 24.9 24.6 25.0 24.5 25.0 24.8 24.8 244 248 24.3 25.0 24.6 pH (SU) 7.87 7.68 7.83 7.55 7.80 7.64 7.88 7.59 7.78 7.61 7.79 7.71 7.89 7.66 DO (mg/L) 8.0 7.9 8.0 7.3 8.0 7.1 7.9 7.4 8.0 7.2 7.9 7.5 8.0 7.6 Conductivity (tmhos/cm) 127 . 128 . . 128 127 'W.* 137 . 138 f 140 ,.

100% Alkalinity (mg1L CaCO3 ) 51 52 :] 56 -.

Hardness (mg/L CaCO3 ) 56 . . 56 . 62 I

Total Residual Chlorine (ma/L) <0.10 <0.10 <0.10

_Temperature (C) 25.1 24.7 25.1 24.6 25.2 24.4 25.0 24.6 25.0 24.4 24.8 24.3 25.2 24.7 PH (SU_) 7.86 7.67 7.82 7.65 7.80 7.65 7.87 7.67 7.79 7.66 7.75 7.77 7.88 7.72 DO.(mg/L) 8.1 7.9 8.11 7.2 8.1 7.0 8.0 7.4 8.0 7.1 8.0 7.5 8.1 7.6 Conductivity (pmhos/em) 125 125 128 . 129 136 138 I 139 100% Intake Alkalinity (mg/L CaCO 3) 50 . . . 54 56 Hardness 5 (mg/L CaCO3 ) 54 . 56 . 58 TotalResidualChlorine(mg/L) <0.10 . <0.10 . . <0.10

_ Temperature (C) 25.2 24.5 25.0 24.6 25.1 24.5 25.0 24.6 25.1 24.4 24.8 24.5 24.9 24.7

Note: Total residual chlorine was performed on non-treated Outfall 101 and Intake samples.

File: sqnl01_051413chem-UV.xts Entered by: J. Suner Reviewed by:

~/;~ Page jof 7.

5n*nrEtlTesiSotln.I.

Total Residual Chlorine (ORION-1977)

VI: Matrix: Water, RL = 0.10 mg/L Meter: Accumnet Model AR25 pH/Ion Meter I.. Analyst Date analyzed

[ '77 6.i1q.IS Iodide reagent: [ r Acid reagent: %%ltg SC;3 SCiig I. Calibration:

ii0 O.lOmg/L I 1.OOmg/L [' Slope Reference standard number %M"0S5 1G4i xms l5oq -tLf. ol I Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range.

if Laboratorycontrol standard:

I Reference standard True value (TV) Measured value (MV) % RS = MV / TV x 100 number (mg/L) (mg/L) (acceptable range = 90 to 110%)

[*t.~ss toc~j 0.50 I r Duplicate amle precision:

Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) I[(S+D)IZ]e x 100 number I(mgNL (acceptable range =.+ 10%)

I! I l?4

,I___"

A Duplicate

54) W' C4AS Lo..60ICO D O.oo? $

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number __ _ _ _ _

Reaet BlankO.o 37 JQl.O1-0 -L C AA q*1iloW, Cl elf eo.ooaIf?

ldi*4.o "02. p~k*ele 4 *i+o, gqf C.o.eo@Z 4~tti o0A A AAwwo i6, iA4 ccft 0I5

¢*'l~o* 001 .d *jcll~e~r~part'des Go~oaofZl

__-____' 0L P4' wclI, CIO%,r, jparrle$ eo.ooWs

_______ ______ O~ w'de *jctlo& j cle.rpwo.Oej.j~

__t__01_ 0 010 +'md pst, .i4I.ycIw0 0oo60l; Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratorycontrolstandard:

Reference standard True value (TV) Measured value (MV) %PRS=MV/TVx 100 number (ing/L) (mg/L) (acceptable range = 90 to 110%)

m lor"i - 0.50 o.l'4 toLt.. I Date reviewed I 0 L1 SOP C8 -Exhibit C8.1, revision 01-01-13 Page 65 of 113

Page ' of L.

"EnTS

(*EnvlronientalTestlng Solutlons, lnr-Total Residual Chlorine (ORION-1977)

Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst [ T A Iodide reagent: %.

Date analyzed oLI1i III Acid reagent:

uS.it1 Calibration:

N:rms i c0.10 mg/L, 1t00 mstL t Slope .

Note: For samples with a residual chlorine of > 1.0 mg/I, the samples must be diluted to be within the calibration range.

Duplicatesample precision:

Sample Sample EI Sample characteristics Residual chlorine %RPD = ((S - D) /[(S+D)/21) x 100 number I .___L (acceptable range =:L 10%)

t~ualsbi -no .-cl ,c1m IS co.@@&(3,

.4 Duplicate D ~o3 Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number mm_/L)

Reagent Blank -- U 3 I*a Irbom-- LY* W.&W 501 jtnA pliIfl glaowr clea, PqArJtls 1o.050Z7 McQlSb..oq- I 101t t- VZ alty iCOW, dcIret paldps P46 IL0.R.41" MI'3C 2Th.0's Ctr no cc(* clcGr 40. got C?

I________ - p4P3oce djfMO4CarPW+cls Co-o.I L Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Reviewed by LIUI Date reviewedI OS4t5 SOP C8 - Exhibit C8.1, revision 01-01-13 Page 66 of 113

Page I of L 6 EnvirmnmentalTesting Solutions, Inc.

Total Residual Chlorine (ORION-1977)

Matrix: Water, RL = 0.10 mg/L Meter: Accumet Model AR25 pH/Ion Meter Analyst M 7 R(

)dide reagent: 3:X (C S¢g.

Date analyzed [4$.III.i3 Acid reagent: -LrI1SC3 Calibration:

~0.10 mg/L I. 1.00 mg/L [ Slope l Reference standard number. z4s* [OCq 0 mss IO*4 -1 Note: For samples with a residual chlorine of > 1.0 mg/L, the samples must be diluted to be within the calibration range.

Laboratorycontrol standard:

Reference standard True value (TV) Measured value (MV) % RS =MV / TV x 100 number (raig/L) (rg/L) (acceptable range i 90 to 110%)

Ir1455 o6Cq 0.50 0.9"T.IC' Duplicatesampleprecision:

Sample Sample ED Sample characteristics Residual chlorine %RPD = (S - D) IK(S+D)121) x 100 number in/L (acceptable. range =: 1O0N 4.Duplicate D 4-'o Z - ,

Sample measurements:

Sample Sample ID Sample characteristics Residual chlorine number 11113)/L Reagent Blank L o~fl3

,. qpt 170 110 iso colo(/ee 4.60193 O

k~iAI C7 I_____ Cfrya cir.

c*.r 'o4c

<oGOZZq

%-Iwi.1O "toQ ' .*I13 Io no cdorsdelmr to.E3oC 4, J'rAYe.4 tar i-1 oCl', -,q/cl ~'o~o$

EO%Stisle. 1 P -L~t d,4(C~o clr;/~n 4

__________~ ____________ 0-_ no c1olor e"c.r, ?t ýclrj 4.o Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laboratorycontrol standard:

Measured value (MV) % RS =MV TV x 100 I

Reference standard True value (TV) number (mg/L) (rag/L) (acceptable range =!90 to 110%)

Z14ss IoN 0.50 6o.50 oo.1.4 Reviewed by [I I Date reviewed SOP C8 -Exhibit C8.1, revision 01-01-13 Page 67 of 113

Page ___of I a Environmental Testing Solutions, Inc.

Total Residual Chlorine (ORION-1977)

Matrix: Water, RL = 0.10 mg/L Meter: Accurnet Model AR25 pH/Ion Meter EXIIZ Analyst Date analyzed Calibration:

-T os[I.i3I 3 Iodide reagent:

Acid reagent:

Reference standard p number a h 1.0 mL theq4 s4mple m l w.a Note: For samples with a residual chlorine of > 1.0 ing/L, the samnples must be diluted to be within the calibration range.

Duplicatesample Drecislon:

Sample Sample ID Sample characteristics Residual chlorine %RPD={(S-D)I[(S+D)/2J)x100 number ----- ) (acceptable range = "10%)

~c~.r -~A c 0 ~ OO I I j Duplicate D I P91 Sample measurements:

sample Sample ID Sample characteristics Residual chlorine number I mg/L) t*O*ct, 0$

r'!cSIS*1.0&t I j

""A._-l.)

Reagent Blank W

t*:W.il tO

. !*IE *jcIIOI, d*,

e* dlrelj deat"

_ _-_.00_C13

_'_o.

___o.Olo N .

.t.-1 ýit. pAT ,w, c~el,r, p*c4clex r4 tg C0o.0o014 Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.

Laborator control standard:

Reference standard True value.(TV) Measured value (MV) % RS = MV/TV x 100 number (mg/L) (mgIL) (acceptable range = 90 to 110%)

~z 6, .0oq $ZY%s Reviewed by I J Date reviewed [I -I 4 j SOP C8 - Exhibit C8.1, revision 01-01-13 Page 68 of 113

Page 37 Page I of 4

ET f onmetnitaTesting Sdufluns, t.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO3 /L Analyst [II Time initiated I.0o Date analyzed 1L6.I2C .I3 Titrate samples to Time completed IS*

pH = 4.5 S.U.

Tifrani ~znmailft and wnukinli~r di~t~,niinaiina

........ n tu and %7"h/"r .. ..

pH of Normality Normality (A) of H2SO 4 pH Factor or Multiplier -

Deionized Titrant check Begin End Total = (5 ml Na2CO 3 x 0.05)/E (N %50000)/100 ml sample water reference standard ml ml ml = 0.25/E = Nx 500

= 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) 9 DAR 5'40 -5SI)17 o0 I Z.Z o.oZo$ lO.Z.

Laborator controlstandard:

Reference standard True value Sample Alkalinity (MV) % RS = MV/TVx 100 number (TV) volume Begin End Total Multiplier (mag CaCO3IL) (acceptable range J

(mg CaCO3 /L) (ml) ml ml ml = 90 to 110%)

VAS5 I14C 100 100 Q-4q ZI-4 4.0 10.2q q.V Duplicatesam le precision:

Sample Alkalinity %RPD,=

Sample Sample ID volume Begin End Total Multiplier (rag CaCOs/L) ((S - D) /l(S+D)/2]} x100 number (ml) ml ml mi (acceptable range =L 10%)

_ _,__ _10_ _ loo. el4 Z4.C 3Z .' 6.Z S 3 Duplicate (B) . t " D ".0%

C vm 13 11 430 I

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (0l) ml ml ml Multiplier (MagCaCO3/L) 0_.__1___o 100 3Z.4 3S.7 I.' 0.. 3,q J 06 ol -13 MIt LA_ 357 41.7 6.O v 9

,OS.DI D, 13' 43 47 <-..Z ,3 M,00 ___ .0 ~O C __ C

___C.0 IC.3 10's Io 100 Lt .I0.-a3 ,_ ig o3 do 6.7 ,_

rbtksaO-, RI'S ,, z0o wI- Vi. I .9 IOSJcs'W K US2 33.4 St'- (to)0 Reviewed by: I & ,I Date reviewed: I O!;-s2- 'N Page 69 of 113 SOP C6 - Exhibit C6.1. revision 06-01-11

Page 3

ET Page _ of 4 Envrooorsal~stig Slutions. ine.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst [ aI5 I Time initiated [ 77]

Date analyzed as-17. Is Titrate samples to Time completed LIZ ]

pH = 4.5 S.U.

Thrant normality and multiplier determination:

-I pH of Normality Normality (M)of H2S0 4 pH Factor or Multiplier zed Titrant check Begin End Total (5 ml Na 2CO3 x 0.05)/E = (N x 50000)/ 100 ml sample water rererence ml ml = 0.25/E =Nx 500 4.5 S.U. number number ____e__ptable range = 0.0180 - 0.0220)

Laborator controlstandard."

Reference standard True value Sample Alkalinity (MV) % RS MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (Mg CaCOsL) (Ml) ml ml ml =90 to 110%)

16Ss MCq 100 100 34.4 V7.5 1.1 01.Z 'R3 93.0o%

Duplicatesam ie precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (ing CaCO3/L) {(S - D) /[(S+D)/21 % 100 numbe% (ml) ml ml ml (acceptable range =: 10%)

_ __ _ _ _ _ _ _ _ _.0 i.S 3.7D , .I Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)

(mg CaCO3/L) (ml) ml ml ml r'M35 I14C zoo Z5 3.7 11.7 '5.o (LI) 10.Z 330 Sample alkalinity (B) Measured spike value (MV) % R MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCOC3 L) = 75 to 125%)

%C0 170 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3/L)

S1.bsI, ni q_-) ChO .o-vý& 2.5 11.7 14.0 Z.3 clI.*q

o *,..._. 7. . IL ' to .7 27 'n*.?c c&W% L 'ljo 1.47 -,7 1_o o_ b tci.73 Z O.Z3 5.5 qI'o C640.

o.6 0a4l l e_-_Al 7-_ 1 1U.9 US. 150 b__ LtA O S 1ia s 5.o -oo

___-3 tS.1 331 . (1 __zoo t3 os t. - n f 4 1 100 31C 33.C 0.06 _________

'SoS'13.C 3 4S82. . C 4~73 Reviewed by:

LZI ) -: Date reviewed: [ .S7..-3 Page 70 of 113 SOP C6 - Exhibit C6. 1. revision 06-01-11

Page 31 0ET Page 3 of ._

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO 3/L AnalystI [ 1 L i Date analyzed I o$.1'.II] Titrate samples to TimeTime completed initiated I _*

pH = 4.5 S.U. aslz't13 Titrantssnnnaliiv and multinlkr d~~1t~rndnation~

pH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na2CO3 x 0.05)/E = (Ni 50000)/100 ml sample

-xferwce standard ml ml ml = 0.25/E =Nx 500

= 4.5 S.U. number num- _- __(E) (acceptable range = 0.0180 - 0.0220)

Laboratory controlstandard:.

Reference standard True value Sample Alkalinity(MV) %RS=MV/TVxIOO number (TV) volume Begin End Total Multiplier (Mg CaCO3/L) (acceptable range (ag CaCOA) (ml) ml ml ml 90 to 110%)

XASS 114 C 100 100 0.0 4. 90 10.t q.*,'

Duplicatesam le precision:

Sample Alkalinity %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) {(S - D) /[(StD)t21) x 100 number (ml) ml ml ml (acceptable range =1 10%)

I*06Efl.1Z.. flt*L 2S 3.89. lI.Z Z.. qC s 0 go.

Duplicate .z L) .S?3 q.3 Matrixspike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mg CaCO3/L) (ml) ml ml ml

Sample alkalinity (B) Measured spike value (MV) %R=MV/ SV x 100 (rag CaCO3/L) MV = A - B (acceptable range

' (Mg CaCO3/L) 75 to 125%)

atqC Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mR CaCOA/L)

V4)os\. 25_ 1Sa.6 Z0.6 (.4 to Z

_____!________3_ 2 Z0.6 ILI Z.4. 9

~ S.LI~I~IZZ.1 2%1. 6.3 54_______

___________ 2~IIIIIIIII j6t 3%.C Ltq _____ _______

-~~-- ~ 53L C.7 ( 5U.7 __E__0 ___

__.._-_2_ 2 l q 4z%. C.o 1O0Izoo

_4.__ 0.3.0 C.1 (.. ....

__, Ito1,

~QS47O- C1evtt I2 a-~L3 Ca. 1(50) %V n0 Reviewed by: Date reviewed: [ 0,

Page 71 of 113 SOP C6 - Exhibit C6. 1. revision 06-01-11

Page p "ETS SEnvronmentl Testing ,uions, Inc.

Page ___qof.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO3/L Analyst [I Time initiated Date analyzed I i. 1" j Titrate samples to Time completed pH = 4.5 S.U. t3 Thtrant normality~and mnultinlier determination:

pH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total - (5 ml Na 2CO3 x 0.05)/E (Nx 50000)1100 ml sample Wa-er- - standard ml ml ml 0.25/E = N x 500 4.5 S.U. number number _-'-___ _ Aacceptable range = 0U0180 - 0.0220) ., .

_____ __,.l C6.tl,13 Laboratory control standard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (nmg CaCO3IL) (acceptable range (mg CaCO[L) (ml) ml ml ml = 90 to 110%)

-X055 114C 100 100 12.3 I. 1

.Ii 104 ci 3.Zo Duplicatesam le precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) {(S - D) /[(S+D)/21} x 100 number (ml) ml ml ml (acceptable range =:k 10%)

I* Duplricate(B) 2 Z7T7 1-3 .0 C4 ODID.0, Matrixspike recover:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mg CaCO 3/L) (ml) ml ml ml raIss tlI'I j.6o 7 %..7.C 3*.4 Io Cs'. lot CoO Sample alkalinity (B) Measured spike value (MV) % R MV/ SV x 100 (Ing CaCOs/L) MV = A-B (acceptable range (mg CaCOs/L) 75 to 125%)

3300 IZIo0 CIZ-0o%

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO31L)

,* *£r,.'*,1e*..

r Ce 3I.C 'S.o G.4 ce.) tO... 3380 1 o620 3.9 4.0 4.9 Zoo0 L06.1 ..S_ _ _ 1.'1 '

i4. 1 C,0.1 T 1'.V

- RAI i.-

F/

Reviewed by:

I Am] Date reviewed: r*.

Page 72 of 113 SOP C6 - Exhibit C6.1. revision 06-01-1 i

Page eES Page _ of I EnvironimentaliTesting Solutions, Inc.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO 3/L Time initiated ,I Analyst -FM Date analyzed [I s.Iz. Titrate samples to Time completed Is4z.

pH = 4.5 S.U.

Titrantnormalty and muldtiplierdetermination:

pH of Normality Normality (N) of H2=S04 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2CO3 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml ml ml = 0.25/iE N x 500

= 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) 5.18 U it 54o 1rS3 1117 Ot 4 ML. . 0.0zo5 102.

Laboratory controlstandard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total ' Multiplier (Ing CaCO3/L) (acceptable range (mg CCO3/L) (M) ml I ml ml a 90 to 110%)

xm*ss ij4q¢ 100 100 lZA Itl. Lq.o 1..q.q[

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOAL)

S.¢ toIo< ICA 36.6 3.1 10.z,3

_36.1 33.t 3.1 4

3I33.7. 30.

1;3.3 1.4 3.1 31.

3Z1 I. _ __ __ __ __ _ __ _ __ __ __ __ __ __,_ __ __ __ __ __ _

- a 9*.l Reviewed by: I d I Date reviewed: I os-.o-ý VJ" Page 73 of 113 SOP C6 - Exhibit C6. 1. revision 06-01-11

Page qT Page __Jof_

0ET Q nlnintIotn foutllns,Inr.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO3/L Analyst [ "0l Time initiated I1010I0 Date analyzed Io.1iiq.13 Titrate samples to Time completed [j14j pH = 4.5 S.U.

Thtrant normaltv and multlDlierdeterminatoi pH of Normality Normality (N) of H2 SO 4 pH Factor or Multiplier Deionized Titrant check Begin End Total = (5 ml Na 2CO3 x 0.05)/E = (N x 50000)/100 ml sample water reference standard ml ml ml - 0.251E = N x 500

= 4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220) 5.5 ZNKSo I*, 1117 0.7. IZ.S 11.3 o.ozo3 10.,.

lk" o.T.2L - - -

Laborator controlstandard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (rag CaCO3/L) (acceptable range

-T'T.-*s 14C (mg CaCO 1100 jL) 00 ;l tZ. mlS ml 1~,

.0 Io.7. 90Z.o6/

9' to 110%)

Duplicatesam ple precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) {(S - D) /j(S+D)/21} x 100 number (ml) ml ml ml (acceptable range =:b 10%)

r54-1..1l 15S- 100 Z1.5 1.47 3.Z 10.Z s 33 S.Duplicate (B) Z7 qI 3.t Matrix spike recover,:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mg CaCO3/L) (ml) ml ml ml

-rtss 114C So Ioo 24.7 325 7.S 14.Z %0 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75 to 125%)

33 47 qL.o%

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCOAL) bs.-.i sh ? W.5 -8.s too So.z. C.o _ _30S__o_0 c-s.I A1 . 30.5 -44.S C.o CI OS. IS.13 05M-S,-.Su.) I.

0.___ 0.0 9

ISO_

5.9 lo I.I 5.9 9.1

.1 C.!

go 93 asV4M'6 J vi.! 7.1 C.ZI __.

Ie4,qq,L... 25 1V.*. 30.Z 3.0 I.ZO Os.-,,- SA*S. too0 3.Z. 39.9 9.7 99

-.. < *."(nqtthr- I is 39. 43.4 I.S (4)' Io Reviewed by: E E-- [ Date reviewed: I020il3.

Page 74 of 113 SOP C6 - Exhibit C6. 1. revision 06-01-11

Page q3 Page TL of .

(~EnvironlmenailTesting Solutions, Inc.

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO 3 /L Analyst LIT's II Time initiated [ _ 7 Date analyzed OCt. 1i3 Titrate samples to Time completed L II os.1913 pH = 4.5 S.U.

Titrant normallty and multidlierdetermination:

pH of Normality Normality (N) of H2SO 4 pH Factor or Multiplier Dia*zed- Titrant check Begin End Total = (5 ml Na2 CO3 x 0.05)/E = (N x 50000)/100 ml sample water reml ml = 0.25/E =Nx500 4.5 S.U. number number __ __acceptable range = 0.0180 - 0.0220)

Laboratorycontrol standard:

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (rag CaCOL) (ml) ml ml ml 90 to 110%)

X.Ss 114C 100 100 (5.0 9.1 '9.1 I(.1 C1 013-00/

Duplicatesample precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCO3/L) ((S - D) /[(S+D)/21) x 100 number (mi) ml ml ml (acceptable range - : 10%)

J lbus%*4-11 )lt 7.5 .1 ms.5 -3 4 Cq, 10 sQ D I O Duplicate (B) S.1~~ 3.4 D IL.7 Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO 3/L)

(mg CaCO3/L) (ml) ml ml ml Zi I114C '00 Z5 11.5 1 o.5 t*0.?.

Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (Ing CaCO3/L) MV= A -B (acceptable range (Mtg CoC03/L) 75 to 125%)

4o 190 9J0%

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mag CaCO 3/L)

,%)!bu 1. 0X% nuvr 3ZS t0.5 Mqo 3.6 C4) 10.zZ 1o lNA ,.- k TeJA G Ois *I. k100 Z4.o Z4.0 _;.0 _ S1

-,-%Can9. / 2 .21,O 34.1 5.1 5_Z

__,_,1"l_ 3 j____ 34.I 39.5 5.4 55 1zo-s 2 1VAsQ. ~,isr i 44___.~s S 5.o SI 01 2___ 4q.6141.7 5. Z__1 53

. 1os6t ,-i *.0

>o-- 5.o _s

.os%3.o n NA v&l ko 1 5.5 Im.2 7.7 __ej 6 24

_ %S-10_,__.__

_o _ _ _ _Z_0 13.7. 17.4 .7Z (z) _._.___

Reviewed by: LI J--

- Date reviewed:

Page 75 of 113 SOP C6 - Exhibit C6. 1. revision 06-01-11

Page 4t4 Page .3 of S"

EnromnlTesigSauorI Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO3 IL Analyst S- I] Time initiated Date analyzed 1i 14 -13 Titrate samples to Time completed oS .lq l3 pH = 4.5 S.U.

Titrantnormalty andmultiplierdetermination:

pH of Normality Normality (N) of H 2SO 4 pH Factor or Multiplier Dnized Titrant check Begin End Total = (5 ml Na2 CO3 x 0.05)/E = (Nx 50000)/ 100 ml sample water retermmv- --*ttdf- ---

a 'L._ __n ml 0.25180 -0.0220

- 4.5 S.U. number number -- _T_____________010__0020 Laboratorycontrolstandard: .,

Reference standard True value Sample Alkalinity (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range (mg CaCO 3/L) (ml) ml ml ml -90 to 110%)

s C 100 1 000 17.4 2-5 q. I %O.Z q'3 3.0/%

Duplicate sample precision:

Sample Alkalinity %RPD =

Sample Sample ID volume Begin End Total Multiplier (mg CaCOsIL) ((S - D) /[(S+D)/2I) x 100 number (ml) ml ml ml (acceptable range =1 10%)

1bya61.d i 5 *oso Z .5 3.9 . q 02 1 01 S 90 Duplicate (B) 30.1* SL S. 4 ./ D jZ sz.

.I-Matrix spike recovery:.

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3/L)

(mng CaCO3/L) (ml) ml m l mi OU00 114 C llmss 1OO So 30.9 34. 9 C.O (7 Io.., 18)0 Sample alkalinity (B) Measured spike value (MV) % R = MV / SV x 100 (mg CaCO3IL) MV = A - B (acceptable range (mg CaCO3/L) =75 to 125%) 0503 Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO 3 L)

b&1,.o1 7QA %AP- ll1,-'T i s 039.- 4Z.1 Z'9 10o)

IO.2 5_9 U* a S .,I I 1 7. 1 qZ.- 45.g 'I.I C3

_____.__0 , -_ L(5.9 qq.* vi_ _ 9 131 oS%!,. O 1 W, 1 0.o0 3.1 .i C3 b ps is _ 3.1 1~ ?.09 lbo0S 1. 11 - .______ Z__ C.0 Cf.( 3.1 %C

%-,os .o6 1tA M*,. t&3T" 100 q.' I14.9 9.7 __

1 .o - t 11. 3 _ Io.s ze.a 101 .o _

Page 76 of 113 Reviewed by:

~zzIz Date reviewed: 105013 SOP C6 - P.xhihit r.6. 1. rL-vtqinn 06~-M -11

Page 45 Page _ of 5 6 [ff.

e~WfmtfMntlITesQgSoUtIqWdII&

Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO3/L Analyst [I dLI Time initiated [ .7 Date analyzed [j .1.j1jg Titrate samples to Time completed LZZ *]

oS.l9{.3 pH = 4.5 S.U.

Titrant normality and multiplier determination:

. pH of Normality Normality (IV)of H2 S0 4 pH Factor or Multiplier ionized Titrant check Begin End Total = (5 ml Na 2CO3 x 0.05)/E (Nx 50000)1100 ml sample water -rete-ren-e- -- tnad ml ml ml =-0.25/E --N x 50

=4.5 S.U. number number ýec..e table range = 0.0180 - 0.0220) a&*I*/

Matrix spike recovery:

Reference standard Spike value Sample Spike alkalinity (A) number (SV) volume Begin End Total Multiplier (mg CaCO3IL)

(mg CaCO3/L) (ml) ml ml -ml soo 0.0 9.C q.00 __0_9._C_9__

Sample alkalinity (B) Measured spike value (MV) %R=MV/SV x100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) - 75 to 125%)

s'1'-7 9 4.o%

Sample measurements:

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml mi Multiplier (mg CaCOIL)

% ll-al 1- 3 1.C 14 Zo.7 S25 5.Z 56

%S6os,.0'L TVA % *aflM _ Zo.! z.5.1 4.9 .56 2.o ZS. 3o04 5.3 _ 'q

.5 ____30.'f 35.9 55 __

~~ ~YVVSXQ VV _ _ 35.9 41A9__.0 G_____

~ P ____ 41.9 1447. 6.0 _ __L 61

__________ 3 _____ 0.0 . _ __ C bj

_________ '.1 t _ _ _ __ 6 Reviewed by: L I, Date reviewed: 1053 -IS Page 77 of 113 SOP C6 - Exhibit C6.1. revision 06 11

Page tI

ET Page 5 of 5 5,rwlrotrenftITesflnig SaluftnImrs-n Alkalinity (SM 2320 B-1997)

Matrix: Water, RL = 1.0 mg CaCO 3/L Analyst I M Time initiated Date analyzed I0S. .is1 Titrate samples to Time completed pH = 4.5 S.U.

l7trantnormalfty and multiplierdetermination:

pH of Normality Normality (N) of H 2SO 4 pH Factor or Multiplier ed Titrant check Begin End Total = (5 ml Na 2CO 3 x 0.05)/E = (Nx 50000)/100 ml sample water re ___Ll ml ml = 0.25E =N x 500

= 4.5 S.U. number number 0.01_0 - 0.0220) __r_) _______ffi_

a3 Gs.qc. 13 Laborator' controlstandard:

Reference standard True value Sample Alkalinity(MV) %RS=MV/TVx 100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL) (acceptable range (mg 1ml)

CaCOAL) m1 ml ml = 90 to 110%0)

ZSV$ 114 C 100 100 121Z. z S . 1 i.T.

10 93 ell-o.o Sample measurements: ....

Sample volume Begin End Total Alkalinity Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L) v50 e0. 2, 100 Z13 %4.o V 1O.Z 4-~ ~~~~~ ~- ___ Y. ____

Reviewed by: LI II Date reviewed: 1C5.

Page 78 of 113 SOP C6-Exhibit C6.1. revision 06-01-11

Page 90

ET Page l of_ __

~n ital~nbTesflng Saiutiorns Inc.

Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3 /L

Analyst pL I I] Time initiated V302 Date analyzed 4)iG jjjq-

" j Time completed iS o Titrantnormalt andmultiplier determination:

Titrant Normality check Begin End Total Normality (A)of EDTA pH Factor or Multiplier reference standard ml ml ml -=0.2/E = (Nx 50000)/50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220) = N x 1000

't -* I3*J 3 0. o 1.O. 10.. 0.00200 Laborator control standard:

Reference standard True value Sample Hardness (MV) % RS = MV /TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCOgL) (acceptable range (magCnCO3/L) (ml) mi ml ml .,=90 to 110%]

40 50 0."O ?b L40 10 Duplicatesam le precision:

Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mrg CaCO3IL) [(S - D) /l(S+D)12j} x 100 number (ml) ml ml ml o6~o r 5V) GO 172.o lq. . 1 Zo-o S 42.

IlDuplicate (B) I [ j IJ

, 14 .0212,I 1 / 1'- q . 1i' ---

Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Irg CaCO3/L)

(Ing CaCO__L) (m ml__l) ml ml five

__ _ _b tq,_I_19-Z t .2.123 2O Sample hardness (B) Measured spike value' (MV) % R =MV /SVx 1O0 (Ing CaCOL) MV_= A-B. (acceptable range (Ing CaCO3AL) = 75 to 125%)...

-q- I 1Wo, oy.

Sample measurements:.

Sample volume Begin. EndI Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier ____mCaCOL)

TV=NDBlank TV=ND (should be = 0 mg* CaCOAL) * **0 O. O (.L) NO0.

O5,*uA0 o3 ql Xq,. ~ 062 2

vbosolll..o wo-shl,*-fh 6"0 P.0 4-4 6-S )1o 1-se*.1 *Crauktnvo *ooJ 2 L L.Z.. IS Z.?- .10

-SIN .o-- .." 4 7- I q5.7 q&,5 13.1 20O Note: If >I 5ml of titrant is used, sample must be diluted. Reviewed by: ) Date reviewedd " k.

Page 79 of 113 SOP C7 - Exhibit C7.1. revision 01-01-13

Page 9I Page 2, of 3

(~EnvlmnynontafTesting SolutIons,Inc.

Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3/L Analyst [i~7I77 Time initiated II Date analyzed [*,]f ,*-I Time completed L ,*tJ Titrantnormality andmulidlier determination:

'Tant-AWrmalfty.- heck Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard m W ml = 0.2/E = (Nx 50000)/50 ml sample number number

e range r 0.0180 - 0.0220) = Nx 1000 Laboratorycontrolstandard:.

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 1001 number (TV) volume Begin End Total Multiplier (Ing CaCO3/L) (acceptable range (rag CaCO3/L) (ml) ml ml ml 90 to 110%)

IZj101o 40 50 2.0 -2.0.0 14 too.O0 I0 Duplicatesample precision:

Sample Hardness %RPD.=

Sample Sample ID volume Begin End Total Multiplier (mg CaCOs/L) ((S - D) /l(S+D)/2J} x 100 number (ml) nl ml ;__

mlT, ___ ___

Duplicate(B) o.Q0. 10.0 L, D N o I Matrix spike recover:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End' Total Multiplier (mg CaCO3IL)

S(mugCaC Sb /QO (ml)3 mJ .ml 7.~o 4'.o

l**.

ml 4 . I0 Sample hardness (B) Measured spike value (MV) I% R = MV /SV x 100 (mg CaCOsL) MV A - B (acceptable range (mg CaCO3 /L) = 75 to 125%)

1L~o 0 SOLo.oI ,

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier _ (mg CaCO3/L)

TV =- fl Blank -,- OI* i*3 (should be = mCaCOL)

,&OSA.0M I Z, I r ,1 A .1 1._ -

_,_,_-*.* `S0- 3.2. IOo

m\l -O0 ISO& nI-L/ *I4.51. -,0 1< -1)

I%- 0 -3. ____I___1-54Q VNIISCA.~~

I_

k.~,1 Ot14 3 4/_ __ '48 LI;3 3,5 '___4jHID Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed I 6is'- I\"-i0 Page 80 of 113 SOP C7 - Exhibit C7. 1. revision 01-01-13

Page O____

SET Page 3 of 3 1 nlneu Te1n 5outlans*Inc.

Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3 /L A n aly st UI -, Time initiated Date analyzed [ 0S. I.

(! Time completed L I Titrantnormalfiv andmultidller determination:

Titrant Normality check e

i- Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml m =.2/E . = (N x 50000)150 ml sample number number (E) (acceptable range - 0- ! = N x 1000 Laboratorly controlstandard:

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3IL) (acceptable range (mg CaCO*_L) (ml) ml ml ml = 90 to 110%)

Duplicatesam le precision:

Sample Hardness %RPD =

Sample Sample ID volume Begin End Total Multiplier (rag CaCO3/L) ((S - D) /l(S+D)t2]) x 100 number (ml) ml ml ml 4~2S~6 l3o D Si 4olr Matrix spike recomerl:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (mg CaCOAiL)

ým.CC3&L

_._ml) mI ml ml I'M56)121ý *. 25-40

., e0 6.0 '00 too Sample hardness (B) Measured spike value (MV) % R MV / SV x 100 (rag CaCO3/L) MV = A - B (acceptable range (mg CaCOjIL) = 75 to 125%)

140 L/O o0*

Sample measurements:.

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (rag CaCO 3L)

Blank . _.......- -LO RI C TV

= liP (should be = 0 mg CaCO /L) bo*t-Whz.*

,-0 UA 3

o, Z3 A-1* A,0~ 6q 3 '333, &0(i33 9.Z

.~~&0-1 ___2. 4C~ eqA Note: If >1 5ml of titrant is used, sample must be diluted. Reviewed by: j( ] Date reviewed [ 0o'.

Page 81 of 113 "

SOP C7 - Exhibit C7.1. revision 01-01-13

Page 93 Page _J_ofL.

"ETS Environmental Testing Solutions, Inc.

Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3/L Analyst LI*IIIII Time initiated ijqC Date analyzed LP. Ij.i Time completed IgSq 4 Thtrantnormality and multiplierdetermination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reference standard ml ml ml = 0.21E = (N x 50000)l50 ml sample number number (E) (acceptable range = 0.0180 - 0.0220). N x 1000 VICfS37 'TSS ioq93 .o 10.0 10.0 o.ozod , o, Laboratorycontrol standard: .

Reference standard True value Sample Hardness (MV) % RS = MV /TVx 100 number (TV) volume Begin End Total Multiplier (Ing CaCO0sL) (acceptable range (Ing CaCO3IL)) ml ml ml '90 to 110%)

1 40 50 IS.o rz.o Z.o zo.U0 40 10O.,%

Duplicate sam le precision:

Sample Hardness %RPD Sample Sample ID volume Begin End Total Multiplier (mg CaCO31L) {(S - D) /I(S+D)t21) x 100 number (ml) ml ml ml so It.o 14.0 z.o Z0.0 SDuplicate(B) 14.0 1 ,O .O '40D 40 -'rEAS.I3 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) volume Begin End Total Multiplier (Ing CaCO3IL)

(Ing CaCO3IL) (ml) mil ml ml

.J U I.

IZ9 40 so I '1..o o.O so Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100 (Ing CaCO3IL) MV = A - B (acceptable range (mngCaCO3/L) = 75 to 125%)

415 L40 to0.0%

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/L)

TV=ND Blank ZOO

.0.0 ND N.D.0 (should be = 0 mg CaCO 3/L) 0 S~i13 fn R's iS.o ZZ.4 4.4 %V*

________, ____ .Z.LItC .. g '1.9 __ _ __ _

0b'TA',31'1 2C.S 31. 4.4 _ _ _

OS,.ib..; Go5.oC, 3- 'iOqO 4 Us., 11- Q5 l4 '*b. VV) I WO 44.3 4.3 SIC

_ %S-_*_. 0.0 4.3 4.3 6C IDS~- %I 4I 1____ 41.3 IV,! JU. Ca___ I_____

Note: If >1 5ml of titrant is used, sample must be diluted.

Page 82 of 113 Reviewed by: zizI~zz Date reviewed 1 0-.-

SOP C7 -Exhibit C7.1. revision 01-01-13

Page t 0ES Page 2. of 0uim1W Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3/L Analyst r Time initiated Date analyzed [jjSjlj.jj Time completed 05.f 6.I3 Titrantnormality and multiplier determination:

Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier reftence standard ml ml ml = 0.2/E = (N x 50000)/50 ml sample number -- nnmber -l (E (acceptable range = 0.0180 - 0.0220) = Nx 10001 I ES-"-I. a Laboratorycontrolstandard:

Reference standard True value Sample Hardness (MV). %RS=MV/TVx100 number .(TV) volume Begin End Total Multiplier (mg CaCO3/L) (acceptable range I (Ing CaCO3 /L) (nil) ml ml ml = 90 to 110%)

J Towss IIZ 40 50 ., Jo1 I Z.o0oo.o%Zoo qo0 Duplicatesam le precision:

Sample Hardness %RPD =

Sample Sample'ID volume Begin End Total Multiplier (mg CaCO3IL) {(S - D) /[(S+D)/2]} x 100 number (ml) ml ml ml

%.d*kS.0%T v*F* 50Ip 10.9 13.S Z. C zo-o '5 Duplicate (B) ms , oc' D -.-- S-* i.

Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3IL)

T3 - Blank (should be = 0 mg CaC0 3IL) _ _ _.__13

05._

%3.O T*I ")op

-VA ,.i low. 70 50.I to.1Z.1 ZZ.o 56 l1.o1. ,- A su t OTt t3.

Z_._ S Z.7 64 o_ is. - q 1.%.S 2 'E9.3 Z.% __

lbosn-.c*3 39.3 'Z.9____1__

bbtsis.o % T ASwt uu _ St.Z 35.0 Z.9 __

Ibcs s.6.o ... , 35, 3S ,. _.7_ SC

'Zom 11. 01 _o.1 37.A. I.

&____~ _ _ _____

1:40Sis aL. I-VA S~tJ WTg0V ______ ____ s54~~1.

Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: LIII.@Z Date reviewed 1o0;1&'5 Page 83 of 113 SOP C7 -Exhibit C7.1. revision 01-01-13

Page 9S Page '3 of ý Enn.1UTestingSclutons, Inc.

Hardness (SM 2340 C-1997)

Analyst LI7* II RL = 1.0 mg CaCO 3 /L Time initiated Date analyzed 06.1,80 Time completed [I I I l OS'-Ig.L Titrantnormaliy andmultiplier determination.

Titrant Normality check Begin End Total Normality (IV)of EDTA pH Factor or Multiplier

-- standard ml ml ml = 0.2/E (N x 50000)/50 ml sample number number - - (E) (acceptable range = 0.0180 - 0.0220) = Nx 1000

3"4 o., lt-.I.,

Laboratorp controlstandard:

Reference standard True value Sample Hardness (MV) % RS = MV / TV x 100 number (TV) volume Begin End Total Multiplier (mg CaCO3.L) (acceptable range (Mg CaCO3IL) (MI) ml mi ml 90 to 110%)

v* 11ZI .40 50 41.S 145.5 1-IMq 20.0 36 . *5

)

.J Sample measurements:.

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3/IL)

T ND BlaCk .

(should be = 0 mg CaCO3IL) - -O- -. ,3 IIoS &1. 61\ TVKA.. ih"1WQ 3 50 4.7 -1.C Z.I Z0O 5" 1_ _US_-1. I I.C . I. q L.,

L4.3 G, 0A I l* wej* 101 ,rcr 1 13 -~ 3.1

1IDoS Is..AN Z Vq.4 , V-8 3.4 I~o*1. 1" .L A.I__27.9 31.5 3.7 7q 12 c 14AiJ&J~r~ V11, _ 31.s 3 3535 &S _ 70

_________ 41 A __55__ W 3e.9 '3-5 _ _ _ 70 Note: If >15ml of titrant is used, sample must be diluted. Reviewed by: Date reviewed I os" 1 "1, Page 84 of 113 .5 SOP C7 - Exhibit C7.1. revision 01-01-13

Page qC SIT Page

of 4 4 Envim,,mental TestingSolutiamsInc.

Hardness (SM 2340 C-1997)

RL = 1.0 mg CaCO 3/L Analyst [I I I Time initiated [ 7 Date analyzed Li.16.13 Time completed Titrantnormality and multin~lierdetermination: $.$1.013 Titrant Normality check Begin End Total Normality (IV)of EDTA pH Factor or Multiplier reference standard ml ml ml f 0.2/E (N x 50000)/50 ml sample number s (E) (acceptable range = 0.0180 - 0.0220) Nx 1000 Laboratorycontrolstandard:

Reference standard True value Sample Hardness (MV) %RS=MV/TVx100 number (gCaCO (TV) IL) volume (l Begin ml.!ml End ml Total 90 to 110%)

3 Multiplier (mg CaCO 3/L) (acceptable range s40 50 3J . 40o. Z.o 1-.00 40 Ioo.oY Duplicatesample precision:

Sample Hardness %RPD-Sample Sample ID volume Begin End Total Multiplier (mg CaCO3IL) ((S - D) /[(S+D)/2)} x 100 number (ml) ml ml ml 1 iCn -Li1 / i 61 3 5

-VAQ,) 1oo.S 4q.0I .S 73..0 S 70 Duplicate (B) ,1 4.0 14 .51 D 70 Matrix spike recovery:

Reference standard Spike value Sample Spike hardness (A) number (SV) " volume Begin End Total Multiplier (mag CaCOIL)

(rmg CaCO3 L) (ml) ml ml ml

'r ms I 112,1 40 s'o 4 1.0 41.S G.5 2o.6 I to Sample hardness (B) Measured spike'value (MV) % R MV / SV x 100 (mg CaCO3/L) MV = A - B (acceptable range (mg CaCO3/L) = 75 to 125%)

70 4o (o0.o0 Sample measurements:

Sample volume Begin End Total Hardness Sample number Sample ID (ml) ml ml ml Multiplier (mg CaCO3iL)

TV= Nr Blank (should be - 0 mg CaCO3fL) __,__ __ __ _ __

___5_1_.___ _ IAW 11' l So .0.0 3 3..1 20,0 CL

_,Lys.IS 2 3.1 C%3 3Z _ C_

boS-1. rL. 3 6C.3 9.7 -3.4 _

i1:O.a(a? q 6-n 61. 10-tS 1.1. ~z

______ d.10l.18 %3.T T. 4 (too) _________

of

_____ S f1 Note: If >1 5lm of titrant is used, sample must be diluted. Reviewed by: [ Date reviewed a .

Page 85 of 113 uS SOP C7 - Exhibit C7.1. revision 01-01-13

Sequoyah Nuclear Plant Biomonitoring May 14-21, 2013 Appendix D Reference Toxicant Test and Control Chart Page 86 of 113

0 0

$ Pimephalespromelas Chronic Reference Toxicant Control Chart Organism Source: Aquatox, Inc.

Environmental Testing Solutions, Inc.

1.0 S I I I I II I I I I I I I I I I I USEPA Control Limits (+/- 2 Standard Deviations) 0.9 0.8 0.7 0.6 0.5 1.4 I I I I I I I I I I I I I I I I I I I I USEPA Warning and Control Limits (7 5 th and 9 0 th Percentile CVs) 1.2 1.0 ..

V 0.8 0.6 0.4 1.4

- Laboratory Warning and Control Limits ( 10 th and 2 5 th Percentile CVs)'

1.2 1.0 0.8 0.6 I 1I I 1 I .. ...I... .... ... ... ... ... I .... .. ... .... ... .. I 0.4 N1 NT N1. O*N% J'q- 1-1"Tes" %N ,N" date" k S"" V 6 " "* N'

Test date

7-day IC2 5 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

Central Tendency (mean IC2 5 )

Warning Limits (mean IC2 5 +/- SA. 10 or SA.75) Graphs generated from associated excel spreadsheet.

Control Limits (mean IC2 5 :+/- SA.25, SA.90, or 2 Standard

Deviations) Excl spreadsheetentered b Reviewed by:

. Sumner

, age 87 of 113

frs co O

OE T Pimephalespromelas Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.

State and USEPA Laboratory Laboratory USEPA USEPA Test number Test date 7-day 1C. CT S Control Limits S&IO Warning Umits S s Control Umits 87 Warning Limits Control Limits S&I. CV (g/L Kd) (g/L KCI) CT-25 CT÷2S CT-S.. CT÷S. CT - SA,25 CT + SA.2s CT - SA.7s CT + SA.,- CT- S.e CT + S.e 1 05-15-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.08 2 06-05-22 0.72 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 056 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 3 06-12-12 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 4 07-10-12 0.76 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07 5 07-17-12 0.73 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.07 6 08-07-12 0.70 0.72 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.07 7 08-14-12 0.81 0.73 0.05 0.63 0.82 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.06 8 09-11-12 0.82 0.73 0.05 0.64 0.83 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.46 1.01 0.33 0.40 1.06 0.07 9 10-02-12 0.66 0.73 0.05 0.64 0.83 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.46 1.01 0.33 0.40 1.06 0.07 10 10-16-12 0.71 0.73 0.05 0.64 0.83 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 11 11-06-12 0.65 0.73 0.05 0.62 0.83 0.09 0.64 0.82 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.06 0.07 12 12-04-12 0.77 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0-58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 13 01-08-13 0.74 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 14 01-15-13 0.76 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.88 0.28 0.45 1.01 0.33 0.40 1.06 0.07 15 02-05-13 0.65 0.72 0.05 0.62 0.83 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.07 16 03-05-13 0.74 0.72 0.05 0.62 0.83 0.09 0.64 0.81 0.15 0.57 0.88 0.28 0.45 1.00 0.33 0.40 1.05 0.07 17 03-05-13 0.79 0.73 0.05 0.63 0.83 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 18 04-02-13 0.77 0.74 0.05 0.64 0.83 0.09 0.65 0.82 0.15 0.58 0.89 0.28 0.46 1.02 0.33 0.40 1.07 0.07 19 05-07-13 0.63 0.73 0.05 0.62 0.84 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 20 05-14-13 0.78 0.73 0.05 0.62 0.84 0.09 0.64 0.82 0.15 0.58 0.89 0.28 0.45 1.01 0.33 0.40 1.06 0.07 Note: 7-d 1Cs = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.

CT= Central tendency (mean IC25 ).

S = Standard deviation of the IC2 values.

Laboratory Control and Warning ULilts Laboratory control and warning limits were established using the standard deviation of the lCs values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPAfor the test method and endpoint.

S*u, = Standard deviation corresponding to the 1 0 "'percentile CV. (S.0 = 0.12)

SA, = Standard deviation corresponding to the 25* percentile CV. (SAs = 0.21)

ISEPA Control and Warning Uimlts

%"~a = Standard deviation corresponding to the 7 5 'hpercentile CV.(SA5 = 0.38)

SA = Standard deviation corresponding to the 90" percentile CV. (S&w = 0.45)

CV= Coefficient of variation of the IC25values.

USEPA.2000. Understanding and Acoountlngfor Method Variabilityln Whole EffluentToxiedtyAppca*ltions Underthe NationalPollutant Dhcharge Elimination Program. EPA-833-R-O0-003.

USEnvironmnental Protectln Agency,Cninnati, OH.

File: ppkclcr_0514l3Adsx Entered by J. numnea Reviewed by i7

0 Pimephalespromelas Chronic Reference Toxicant Control Chart Precision of Endpoint Measurements 3 Environmental Testing Solutions, Inc.

Organism Source: Aquatox, Inc.

10 . . .

I

- I I I A cI C ri I I I I I I I aI I I) 1,00 0.75 "2 0.50 0 E. 0.25 USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)

- I I iI I i I I I, I I I I i i I I I I I I i I I I I I I I I I I I I I I 30 Kentucky Acceptance Limit (< 30.0%)

C--

20 0..

CO 10 0 0 I I I I I I I I I I I I I ! I I I I 0 -

"1 I -

I I I i I I I i . . . .

I S I I I I I I . I I I I I 1 I I 0 I I I I I3 30 USEPA Upper PMSD Bound (90'* percentile < 30.0%)

20 Q

10 n I - I I I I I I I I I I I I I I I I I I I Test date Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD)

Control Limits (mean Control Growth, CV, or PMSD +/- 2 Standard Deviations)

Rby Graphs generated from associated excel spreadsheet.

Excel spreadsheet entered by: J. Sumner age 89 of 113 Reviewed by:

U"

of Endpoint Measurements "ETS EnvironmtalTesting Solutions, c.

Precision( mephales promelas Chronic Reference Toxicant Data Control Control Mean Test number Test date Survival Growth CT CV CT MSD PMSD Cr for Control Growth for Control

(%) (mg/larvae) (mg/larvae) (%6) Growth CV (%) (%) forPMSD (%)

1 05-15-12 100 0.697 5.9 0.07 9.5 2 06-05-12 97.5 0.596 0.647 3.8 4.9 0.06 9.4 9.5 3 06-12-12 100 0.723 0.672 6.0 5.2 0.11 15.6 11.5 4 07-10-12 100 .0.641 0.664 5.2 5.2 0.10 14.9 12.4 5 07-17-12 100 0.638 0.659 10.9 6.4 0.07 11.4 12.2 6 08-07-12 97.5 0.660 0.659 6.7 6.4 0.06 8.7 11.6 7 08-14-12 100 0.533 0.641 5.4 6.3 0.07 13.1 11.8 8 09-11-12 100 0.599 0.636 4.2 6.0 0.06 10.1 11.6 9 10-02-12 100 0.558 0.627 3.9 5.8 0.04 7.0 11.1 10 10A6-12 97.5 0.550 0.620 6.7 5.9 0.09 15.5 11.5 ii 11-06-12 100 0.615 0.619 5.9 5.9 0.04 7.1 11.1 12 12-04-12 100 0.601 0.618 3.3 5.7 0.06 10.1 11.0 13 01-08-13 100 0.549 0.612 3.3 5.5 0.06 11.0 11.0 14 01-15-13 100 0.532 0.607 4.2 5.4 0.07 13.0 11.2 15 02-05-13 100 0.487 0.599 7.4 5.5 0.06 12.8 11.3 16 03-05-13 100 0.504 0.593 8.5 5.7 0.07 14.7. 11.5 17 03-05-13 100 0.564 0.591 9.1 5.9 0.10 16.9 11.8 18 04-02-13 100 0.582 0.590 6.5 5.9 0.12 19.9 12.3 19 05-07-13 100 0.651 0.594 7.8 6.0 0.08 11.9 12.2 20 05-14-13 100 0.599 0.594 5.7 6.0 0.08 12.8 12.3 Note: CV = Coefficient of variation for control growth.

Lower CV bound determined by USEPA (10th percentile) = 3.5%.

Upper CV bound determined by USEPA (90'h percentile) = 20%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD Isa measure of test precision. The PMSD Isthe minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10 percentile) = 12%.

Upper PMSD bound determined by USEPA (90t percentile) = 30%.

CT = Central Tendancy (mean Control Growth, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.

EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2 Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

File: ppkclcr051413.xlsx Entered by: J. Sumner Reviewed by:

tage 90 of 113

Page 1 of 6 0EvmnmemmIT"Utng sdutiomrK.In Potassium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1000.0)

Species: Pimephalespromelas PpKCICR Test Number: V.8 Dilutionpreparationinformation: Comments:

KCI Stock INSS number: 1111'L-Stock preparation: 50 gKCI/L:

Dissolve 50 KC in 1-L Milli-Q water.

Dilution prep (mg/L) 300 450 600 750 900 1050 Stock volume (mL) 6 9 12 15 18 21 Diluent volume (mL) 994 991 988 985 982 979 Total volume (mL) 1000 1000 1000 1000 1000 1000 Test organism information: Test information:

Organism age: U 4 os Randomizin template: _ __.p,_

Date and times organisms were - t Incubator number and born between: shelf location: _

Organism source: t %fw., 0ml. Artemia CHM number: C lJa'i1 O Drying informationfor weight determination:

Transfer vessel information: pH = "VIS S.U. Date / Time in oven: os-zV &b W Temperature = " 0C Initial oven temperature: 60 (.

Average transfer volume: Date / Time out of oven: o.'L'LS A4'0 Final oven temperature: 60 *C, 0 Total drying time: ____,o_0_.

Dailyfeeding and renewal information:

Day Date Morning feeding Afternoon feeding Test initiation, renewal, MHSW or termination batch used Time Analyst Time Analyst Time Analyst 0 os. 4 LoC .%.S OS-_Is 2 os_._. _ Also LA **Is-4*13 JL I'oo A 6 'if ps - n.,JS A O *7. '15 o~tils 7 1S-2-%.

Controlinformation: Acceptance. criteria Summary of test endpoints:

%Mortality: p v a.

20% r e 7-day LCm L.C -

Averae weight per initial larvae: A.. Sig NOECto Average weight per surviving larvae: .; >0.25 mg/arvae* LOEC lO IChV 6104.

IC25 i i%_A SOP AT21 -Exhibit AT21.1, revision 06-01-11

- age 91 of 113

Page 2 of 6 0ET Species: Pimephalespromelas PpKC1CR Test Number: OVA Survival and Growth Data Day .Control 300 mi KCI/L 450 mg KCV/L A B C D E F G I H I J K L 0 to010 i I0 0I 10 101o to 101 t10 1 l 0b Ito ' 10 6b tO 10 to 10 10 2 '0 10 J O1 1 0Q 10 1 '0 C) 3 t1 10 (0 10 tO tO 1O O t000 O 4 1 10 1. I10 10 1 0 (o0 t0 10 10 10 I Io _1_ 1L) o (to Io 1to to O10 6_ 1_ 1_ I 113to 10 10 10 10 Io to 7 10. /0 I0 10 10 /0 i0 Io '0 to /0 to A = Pan weight (mg)

Tray.color code:: m enla Analyst: _ MSU Date: 05.0p-l3 B = Pan + Larvae weight (Mg)

Analyst:

Date: O.i*ZZ-13 I Average weight per Initial Percent number of larvae (mg) reduction from control I(%) I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk =.sick, sm = unusually small, Ig = unusually large, d&r - decanted and returned, w = wounded.

Comments:

SOP AT21 - Exhibit AT21.1, revision 06-01-11 t~age 92 of 113

Page 3 of 6 ETS Species: Pimephalesoromelas PpKC1CR Test Number: 2Lýj I I I

I Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,.

Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

SOP AT21 - Exhibit AT2I. 1, revision 06-01-11 age 93 of 113

Page 4 of 6 0Ennnmn.Te~~~Sottt.I Species: Pimephalespromelas PpKC1CR Test Number: 1_16&

Survival and Growth Data Day 1050 mg KCi/L Y Z AA BB 0 I to t0 1 to

-2 %A,* q'lotJ I1 4 4 55__ _ _ bt "1. !L 6 A .. "Z' 7 i.-L '

- - 514 A = Pan weight (mg)

Tray color code::

Date:'

Analyst:

Date:

.inqren'i MW 5og.l3 .d.'

13.75 13.9 I1R /I 1j' 1q.Iq IL.-"~'

1II gt I

B = Pan + Larvae weight (mg)

Analyst:

Date:

______._313.1--/_iIU9 o£.Z-l3 I . .

C = Larvae weight (mg) = B - A Hand calculated. oq oi' t.b2. '-I'L Analyst:

Weight per Initial number of larvae (mg)

= C / Initial number of larvae Hand calculated.

Analyst: _*

e .

Average weight per initial Percent number of larvae (mg) reduction O..(i from control 0 01 Comment codes: c clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

SOP AT21 - Exhibit AT2 1.1, revision 06-01-I1 (fPage 94 of 113

(D Pimephalespromelas Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1000.0 Quality Control Verification of Data Entry, Calculations, and Statistical Analyses

..niro T ... So menta SEnvironmental Testing Solutions, Inc. Test number 268 Test dates: May 14-21, 2013 5

Csmtnesljos*mlgLl balceP.

WII -I5inb.of Pralnumb-0of A.PM.,uftM(ms) B Pant turi.-n.

.I0laht40 W.0I,/eWtJI M11fne1e9S1e/gstng cioeateoatu5omn W.lutt/Inlnsunl'otf M01-lydn Musertwinitid Caeidtetwrelot rntnaseofeufrMm hsn- -%ht mle A-B n.nb.,eofIn.e (mInming) mb~rohlegm)m)

IOln P..Wof Ni.. a1-f umt-rofft (114 omtrO (1)

A 10 10 13.22 19.01 5.79 0.579 0.579 Control B 10 10 13.19 19.19 6.00 0.600 0.599 5.7 0.600 10.0 0.599 5.7 Not applicable C 10 10 10.57 19.27 5.70 0.570 0.570 D 10 10 14.07 20.53 6.46 0.646 0.646 E 10 10 13.97 20-39 6.42 0.642 0.642 300 F 10 10 12.60 13.66 6.06 0.606 0.623 3.3 0.606 100.0 0.623 3.3 -4.0 0 10 10 13.15 19.19 6.04 0.604 0.604 H 10 10 12.88 19.26 6.38 0.638 0.638 I 10 10 13.02 19.11 6.09 0.609 0.609 4S0 J 10 10 14.27 21.13 6.86 0.686 0.611 6.70.686 10N.0 0.611 8.7 -2.0 K 10 10 14.20 19.87 5.67 0.567 0.567 L 1 10 10 14.03 19.84 5.81 0.581 1 0581 M 10 9 13.96 19.99 6.03 0.670 0.603 N 10 8 13.40 17.92 4.52 0.565 0.597 83 0.452 90.0 0.537 12.6 10.3 0 10 10 13.39 19.18 5.79 0.579 0.579 P 10 9 13.13 58.28 5.15 0.572 0.515 1 10 8 13.15 17.96 4.81 0.601 0.481 R 10 6 13.54 17.97 4.43 0.738 044372.5 0.50 14.6 16.4 T 10 8 1293 1941 6.08 0.760 01608 U 10 4 15.64 18.40 2.76 0.690 0.276 900 V 10 3 14.72 16.94 2.22 0.740 0.724 21.2 0.222 3S.0 0.248 12.9 58.5 W 10 3 12.97 15.73 2.76 0.920 0.276 X 10 4 13.26 15.45 2.19 0.548 , 0.219 Y 10 2 13.75 14.68 0.93 0.465 0.093 1050 Z 10 *1 13.49 14.23 0.74 0.740 0.661 42.0 0.074 12.5 0.078 34.1 87.0 AA 10 1 12.95 13.97 1.02 1.020 0.102 BB 10 1 14.11 14.53 0.42 0.420 1 1 0.042 Dunnett's MSDvalue: 0.0768 MSD= Minimum Significant Difference PMSO: 22.8 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD Is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (lth percentile) = 12%.

Upper PMSD bound determined by USEPA (90th percentile) 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPAs WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2001a, 20D0b. Final Report Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes land 2-Appendix. EPA-821-B-01-004 and EPA-821--01-005. US Environmental Protection Agency, Cincinnati, OH.

File: ppkcicrl05]43.xisx Entered by: J. Sumner Remiewed by:

ET Statistical Analyses Larval Fish Growth and Survival Test-7 Day Survival Start Date: 511412013 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 5121/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pnimephales promelas Comments:

Conc-mglL 1 2 3 4 D-Control 1.0000 1.0000 1.0000 1.0000 300 1.0000 1.0000 1.0000 1.0000 450 1.0000 1.0000 1.0000 1.0000 800 0.9000 0.8000 1.0000 0.9000 750 0.8000 0.6000 0.7000 0.8000 900 0.4000 0.3000 0.3000 0.4000 1050 0.2000 0.1000 0.1000 0.1000 Transform: Arcsin Square Root Rank I-Tailed Number Total Conc-mglL Mean N-Mean Mean Min Max CV% N Sum Critical Reap Number D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 0 40 300 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00 0 40 600 0.9000 0.9000 1.2543 1.1071 1.4120 9.935 4 12.00 10.00 4 40

750 0.7250 0.7250 1.0229 0.8881 1.1071 10.398 4 10.00 10.00 11 40

900 0.3500 0.3500 0.6322 0.5796 0.6847 9.597 4 10.00 10.00 26 40

1050 0.1250 0.1250 0.3572 0.3218 0.4636 19.881 4 10.00 10.00 35 40 Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates non-normal distribution (p <= 0.01) 0.89176 0.896 0.08591 1.68612 Equality of variance cannot be confirmed Hypothesis Teat (1-taill 0.06) NOEC LOEC ChV TU Steel's Many-One Rank Test 600 750 670.82 Treatments vs D-Control Maximum Likellhood-Problt Parameter Value SE 95% Fiducial Limits Control Chi-Sq Critical P-value Mu Sigma Iter Slope 10.5215 1.38357 7.84887 13.1941 0 1.16914 9.48773 0.88315 2.91814 0.09504 3 Intercept -25.703 3.97572 -33.496 -17.911 TSCR 1.0-Point Probits mglL 95% Flducial Limits EC01 2.874 497.782 415.931 555.987 0.9 EC05 3.355 577.845 506.211 628.394 0.8 ECIO 3.718 825.664 561.398 671.807 0.7 EC15 EC20 EC25 3.964 860.143 601.448 703.066 4.158 688.898 634.794 729.704 4.328 714.56 684.335 753.969 1 0.6 0.5 CJ EC40 4.747 783.548 741.183 822.982 "

EC50 5.000 828.218 787.641 671.902 0.4 EC60 5.253 875.434 833.371 927.764 0.3 EC75 5.674 959.954 908.252 1036.68 0.2 EC80 5.842 995.716 938.1 1085.33 EC85 6.038 1039.09 973.331 1145.88 0.1 EC90 6.282 1096.35 1018.64 1227.96 0.0 /

EC95 6.645 1187.07 1088.41 1362.18 1 10 100 1000 10000 EC99 7.326 1378 1229.87 1658.24 Dose mgIL Dose-Response Plot 0.9 0.8 0.7 0.8 A 0.6 90.4 0.3 0.2 0.1 0

File: ppkclcr_051413.xlsx Entered by: .J. Su~nner Reviewed by:

I age 96 of 113

Eow,..ThnSo.In Statistical Analyses Larval Fish Growth and Survival Test-7 Day Growth Start Date: 5/14/2013 Test ID: PpKCICR Sample ID: REF-Ref Toxicant End Date: 5/21/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: KCL-Potasslum chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Plmephales promelas Comments:

Conc-mglL 1 2 3 4 D-Control 05790 0.6000 0.5700 0.6460 300 0.6420 0.6060 0.6040 0.6380 450 0.6090 0.6860 0.5670 0.5810 600 0.6030 0.4520 0.5790 0.5150 750 0.4810 0.4430 0.4710 0.6080 900 0.2760 0.2220 0.2760 0.2190 1050 .0.0930 0.0740 0.1020 0.0420 Transform: Untransformed 1-Tailed Isotonic 0

Conc-mg1L Mean N-Mean Mean Min Max CV /% N t-Stat Critical MSD Mean N-Mean D-Control 0.5988 1.0000 0.5988 0.5700 0.6460 5.664 4 0.6107 1.0000 300 0.6225 1.0397 0.6225 0.6040 0.6420 3.259 4 -0.708 2.290 0.0768 0.6107 1.0000 450 0.6108 1.0200 0.6108 0.5670 0.6860 8.697 4 -0.358 2.290 0.0768 0.6107 1.0000 600 0.5373 0.8973 0.5373 0.4520 0.6030 12.637 4 1.834 2.290 0.0768 0.5373 0.8798 750 0.5008 0.8363 0.5008 0.4430 0.6080 14.635 4 0.5008 0.8200 900 0.2483 0.4146 0.2483 0.2190 0.2760 12.917 4 0.2483 0.4085 1050 .0.0778 0.1299 0.0778 0.0420 0.1020 34.132 4 0.0778 0.1273 Auxiliary Tests Statistic Critical Skew Kurt Snaplro-WilKs lest Inoicates normal alstribution (p > 0.0u) 0.96436 0.844 0.11667 -0.0895 Bartlett's Test Indicates equal variances (p = 0.29) 3.74779 11.3449 Hypothesis Test (1-tall, 0.06) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 600 >600 0.07678 0.12823 0.00577 0.00225 0.10344 3,12 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mq/L SD 95% CL(Exp) Skew IC05 512.38 48.38 346.57 683.57 0.9162 IC10 574.77 57.18 449.28 858.61 1.1978 IC15 674.73. 69.11 470.86 823.64 -0.0055 1.0 IC20 757.26 52.65 485.54 795.15 -1.5179 0 IC25 775.40 20.36 692.50 809.44 -1.6735 IC40 829.81 10.01 792.62 850.94 -0.2375 0.8/

IC50 866.09 8.39 837.29 890.11 -0.0572 0.7:

0.8.

0.5:

0.4*

0.3; 0 500 1000 1500 Dose mg/L Dose-Response Plot 0.75 0.6

o 0.5: 1-tall, 0.05 level of significance 80.4 0.3 0.2 0.1 File; ppkclcr-0514l3.xIsx Entered by. J. Sumner Reviewed by;

Page 97 of 113

Page 5 of 6 10t 11 nI, nwtIet~gSI0I~,Ic Species: Pimephalespromelas PpKCICR Test Number: *o_.

.Daily Chemistry:

U Day (Analyst identified for each da V,performed pH, D.O. and conductivity measurements only.)

1..2 Analyst II AIfAiv- ~aEific 11 Mb ii Concentration [ Parameter pH (S.U.) 1.q 1 II -7.gi

~

7MI

~ ~ 11~-7.9~ ~ 7.17 lIi "ZT DO (mg/L) - ~~ 7.7~ ~

.----- ~ *-------I.--- A 11 Conductivity 31 3c497 (pmhos/cm) _______

CONTROL *Alkalinityt '

(Mg CaCO3IL) 1

Hardness (mg CaC03Q 3 ) ____

Temperature (Q0) -!.4.'1 pH (S.U.)

DO (mg/L) 7,7

.300 mg KCIIL Conductivity ls (gmhos/cm) S3,""

Temperature (00) *L.A., -.2 A PH a(u.) 7.45 If 7.7__

DO (mgfL)777.

450 mg KClIL Conductivity I .,

(pnmhos/cm) U0

Temperature CC) 1b4. &

S V .-.-

DO (mgfL) .

600 mngKCI/L Conductivity (gtmho /m) 136_____ ________ 370___

_______*Temperature (DC) 1I4.I 2q.I6 U4 1A... A .1) pH (S.U.) %IS__ . q ~ 17 '7M9 DO (mggL) K.7 750 mg KCI/L Conductivity 0. _

(pmhos/cm) (C) 1510

Temperature (00) L4.'i tq..s -A . 2.

PH (S.U.) 7. 1 7 7.170 7. 7. 3 DO (mg/L) 7.6 oil I 7.

900 mg KCI/L Conductivity 18 (A+/-mhos/cm)loo10

__________*Temperature (00) M.%. 1. .e

-Li .. U .4.E DH I

(S.U.)

--- i i 1.Iq 11I 7,7q1 -I1 11f -I7.74 -I 7.97. I .*.I7M DO (mgWL) 1.I 11 Ja 9 1050 mg KCIIL Conductivity (pmho/m I1s o

Temperature (0)Q STOCK Conductivity

ýgrnboslOm Initial 11 Final I

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1.

Alkalinity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:

SOP AT21 - Exhibit AT21.1, revision 06-01-11 age 98 of 113

Page 6 of 6 AmnrnentalTesting Inc Wu1tlons, Species: Pimepha~!es promelas PpKCICR Test Number: ____

Day (A-1h,, # M-tif-A f- -, An. A Th n1 n-A A, AA ,o., oo.4 ~

3 4 5 6 Analyst I A 11TI ma; II-Tg 3'a 1Ii' -st 11us Concentration I Parameter pH (S.U.) 7.47

.3 -~------1I------------

1171.7 I -

7.99q 1 1.7 6.00 11-. i0 0 - - .3 7.93 U

[I 7.'

IM

. 7.%7 DO (mg/L) 7.7 ' II 7.7 Conductivity I '374 (limhos/cm)

Alkalinity

-j 10 k 0611 CONTROL (rmg CaCO3L)

Hardness (mg CaCO3 /L)

Temperature U.74.11I --vks I .91 II .- I -1A9 11ti

-1 -r II *¶ I UOAl 11-L4*. I pH (S.U.) ".,qC .IZ-q 7 7 CO 7. 7 .77 7.qq -7T DO (mg/L) 1. "S. 7S. ' 9 .C j7m 1.o7.5 300 mg KCI/L Conductivity (jimhos/ c) 1667lai

Temperature L -We pH (S.U.) I1.4 77 " 7.q .. , 17.75 -1"93 ?_.Cq DO (mgJL) '7., 6.c .".0 S 7.5 450 mg KCI/L Conductivity

Temperature -q,* "L.-tj b.I.1 ' . LqV. - ,

pH (S.U.) 7.7 7.-7 7,7 . '1 7.1Z 7.75 7-13 1.75 DO (mgL) 7.1 ., 1. ,4 So S.5 7. . .o

.600 mg KCIIL Conductivity 1310 30M Temperature

"]ql ".4~ "".1 lq q- . f -V4.* "~q' -.3 PH (s.u.) ".1A7_ 7..q 7.7 -7.R Z 7.75 " 7 Aq "3lq DO (mg/L) 7.1 7 . 1'0 1?.7 .o 7.l 750 mg KCI/L Conductivity

Temperature 1'- -q* "*l ".,* "* /q/ ' !4 pH (S.U.) -7q7 - 7.9 7. 7%q ' .T1 "7.93 _,__

DO (mg/L) 1. ".'! .7.__7.3 7 '9. Z6, 900 mg KCI/L Conductivity 1,30 0

Temperature "L4.* "7tU1Mie 45 "L.j " %j.-

pH (S.U.) 7.97 7.777 7.-qc I.S -. 1.17 -qz 7.17C DO (mg/L) 7.q 7..-I 70 7. 7,7 t 5 1050omg KCI/L Conductivity

- Temperature .. q L. lq "LI14.9 7q*1 "tq1 a .7.

I¢(c) i Initial Final Initial Final Initial Final Initial Final Iniil Fnlm Initia Fia

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table locatid on Page 1.

4*l ity and hardness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by: * .

SOP AT21 - Exhibit AT21.1, revision 06-01-11 f*-age 99 of 113

Ceriodaphniadubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc.

I I I I I I I I I I I I I I I ! II I I 1.14 USEPA Control Limits (+ 2 StandardDeviations) 1.12 1.10 1.08 1.06 1.04 1.02 I I I I I I III I I I I I I I I I I I 2.5 S I I I I I I I I I I I I I I I I I USEPA Warning and ControlLimits ( 7 5 th and 9Oth PercentileCVs) 2.0 z . ................... ... *.......

1.5 Jn 1.0 Nu 0.5 - .................................................... ..

1.4 I I I I I I I I I I I I I I I I I I I I 1.3 Laboratory Warning and ControlLimits (10th and 25th PercentileCVs)

S......................................

1.2 1.1 1.0 0.9 ...............................................................................................

AQ U.,U Test date 0 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.

- - Central Tendency (mean IC 25)

Warning Limits (mean IC 25 +/- SA. 0 or SA7S)

Control Limits (mean IC25 + SA.25, SA.90' or 2 Standard Deviations)

I I Graphs generated from associated excel spreadsheet.

Excel spreadsheet tntered by: J. Sumner

"*j-age 100 of 113 Reviewed by:

0 Ceriodaphnia dubio 0 *Chronic Reference Toxicant. Control Chart W,) Environmental Testing Solutions, Inc.

State and USEPA Laboratory Laboratory US6PA USEPA Test number Test date 7-day lC-, Cr S Control Limits S&M Warning Limits SA25 Control Limits S. Warning Limits SAM Control Limits CV (8/L Naa) (g/L NaC) CT-2S CT+z2S" - So CT+ S, CT-S, Cr +$S CT -S*. CT + SLý CT-SAjo CT+S,.,

1 01-10-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.01 2 02-07-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 3 03-13-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.01 4 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 5 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 6 05-08-12 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 7 05-08-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 8 06-05-12 1.05 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 9 07-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01 10 08-07-12 1.08 1.06. 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 11 09-11-12 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01 12 10-02-12 1.04 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 13 11-06-12 1.05 106 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 *0.40 1.72 0.01 14 12-04-12 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 15 01-08-13 1.07 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 16 02-05-13 1.05 1.06 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 17 03-05-13 1.05 1.06l 0.01 1.04 1.08 0.08 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 18 04-02-13 1.07 1.06 0.01 1.04 1.08 0.09 0.98 .1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 19 05-07-13 1.07 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 20 05-07-13 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.58 1.54 0.66 0.40 1.72 0.01 Note: 7-d iC 2 = 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodophnio reproduction for the test population.

CT = Central tendency (mean IC25).

S = Standard deviation of the ICý values.

Laboratory Control and Warning Limits Laboratory control and warning limits were established using the standard deviation of the IC, values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and warning limits recommended by USEPA for the test method and endpoint.

S. = Standard deviation corresponding to the 10& percentile CV. (S. = 0.08)

S = Standard deviation corresponding to the 25P percentile CV. (SAs = 0.17)

USEPA Control and Warning Limits

==SStandard deviation corresponding to the 75P percentile CV. (SA,,, 0AS)

S = Standard deviation corresponding to the 90F percentile CV. (SASo=0.62)

CV = Coefficient of variation of the IC. values.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

File: CdNaClCRo507"3new-ywldsx Entered by: J. Sumner Reviewed byx*._

Ceriodaphniadubia Chronic Reference Toxicant Control Chart Environmental Testing Solutions, Inc. Precision of Endpoint Measurements

.40 0 35 U

U 2 30 0 U U ii, 25 0 20 0

0 15 Minimum Acceptance Criteria (> 15.0 offspring per surviving female).

I I I I I I I II I I I j I I I I I I I I I I I 40 North Carolina Acceptance Limit (< 40.0%)

' 30 Kentucky Acceptance Limit (< 30.0%).

~20 I.

102 ................................................................. .. .........

50 USEPA Upper PMSD Bound (90'h percentile < 47.0%)

40 30 20 10 . .......................................................... ..

O

~~v'189 1 1 1 V10 v"'%Nip~Al A~b%F4A

- 0A0 % Qk %S %-S Test date

- Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference (PMSD) PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.

-- *Central Tendency (mean Control Reproduction, CV, or PMSD)

Control Limits (mean Control Reproduction, CV, or PMSD +/- 2 Standard Deviations)

Graphs generated from associated excel spreadsheet.

Excel spreadsheet endered by: J. Sumner Reviewed by:

ýýage 102 of 113

Precision of Endpoint Measurements 0 T Ceriodaphniadubia Chronic Reference Toxicant Data Environmental Testing Solutions, Inc.

Test Tsdae Control Control Mean Survival Reproduction Cr Cv Cr MSD PMSD CT number for Control Mean for Control

(%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%)

(offspring/female) CV (%)

1 01-10-12 100 32.4 5.1 2.4 7.4 2 02-07-12 100 31.7 32.1 4.2 4.7 2.1 6.5 6.9 3 03-13-12 100 31.1 31.7 7.2 5.5 1.9 6.1 6.6 4 04-10-12 100 29.4 31.2 6.8 5.8 2.4 8.0 7.0 5 04-10-12 100 30.3 31.0 7.1 6.1 2.7 8.8 7.3 6 05-08-12 100 31.7 31.1 6.1 6.1 1.9 6.1 7.1 7 05-08-12 100 32.4 31.3 5.1 6.0 2.0 6.1 7.0 8 06-05-12 100 32.1 31.4 4.0 5.7 2.3 7.1 7.0 9 07-10-12 100 31.2 31.4 4.7 5.6 2.0 6.3 6.9 10 08-07-12 100 29.2 31.2 3.5 5.4 1.9 6.6 6.9 11 09-11-12 100 30.7 31.1 6.2 5.5 2.3 7.4 6.9 12 10-02-12 100 32.5 31.2 3.0 5.3 1.5 4.6 6.7 13 11-06-12 100 32.5 31.3 3.6 5.1 1.8 5.4 6.6 14 12-04-12 100 31.9 31.4 5.2 5.1 1.9 6.0 6.6 15 01-08-13 100 30.8 31.3 5.5 5.2 2.0 6.5 6.6 16 02-05-13 i00 32.1 31.4 6.3 5.2 2.0 6.1 6.6 17 03-05-13 100 31.4 31.4 4.8 5.2 1.7 5.6 6.5 18 04-02-13 100 28.7 31.2 5.9 5.2 1.9 6.8 6.5 19 05-07-13 100 30.4 31.2 8.4 5.4 2.2 7.3 6.6 20 05-07-13 100 32.2 31.2 6.2 5.5 2.4 7.3 6.6 Note: CV = Coefficient of variation for control reproduction.

Lower CV bound determined by USEPA (10'h percentile) = 8.9%.

Upper CV bound determined by USEPA (90th percentile) = 42%

MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Lower PMSD bound determined by USEPA (10th percentile) 13%.

Upper PMSD bound determined by USEPA (90h percentile) = 47%,

CT = Central Tendancy (Mean Control Reproduction, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005. US Environmental Protection Agency, Cincinnati, OH.

File: CdNaClCR_050713new-ywt.xlsx Entered by: J. Sumner Reviewed by:

- age 103 of 113

Page 1 of 6

ET Sodium Chloride Chronic Reference Toxicant Test (EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphniadubia CdNaCICR #:

K~t4WVLO Wv g Dilutionpreparationinformation: Comments:

NaCI Stock INSS number: IQ%_ 1_0 _

Stock preparation: 100 g NaCIIL:

Dissolve 50 g NaCI in 500 mL Milli-Q water.

Dilution Prep (mgtL) 600 800 1000 1200.1 1400 Stock volume (mL) 9 12 15 1 18 1 21 Diluent volume (mL) 1491 1488 1485 1482 1479 Total volume (mL) 11500 500 1500 1500 1500 1 Test organism source information: Test information:

Organism age: < 24-hours old Randomizing template color:

Date and times organisms were bom "$1 -ft" omi 7. Incubator number and shelf between: location:

Culture board: OA 'o-. A Rep lic ate n um ber: 1 12 3 14 5 Culture.boardcup number: I" I a I I1SI 16 AI 1 7 1 8 I TV 9 !.&_________

0 10 YWT batch:

it .l 0 4A. %! 0 Transfer vessel information: pH= *f S.U. Temperature= "LO1* C Selenastrum batch: 01A Aver e transfer volume rnL): ________________

Daily renewal information:

Day Date Test initiation and feeding, MHSW Analyst renewal and feeding, or batch used termination time 0 _ rla oia 0__01

.2 oqe os-6t.. WU I 5__ 0%__1_t._ oils os.o'mILN

.1 Control information: Acceptance criteria Summary of test endpoints:

%of Male Adults: 01. -520% 7-day LC50 '7 1q0_0

% Adults having 3'd Broods: 10I0t,. a 80% NOEC !00

% Mortality: '20% LOEC Iwo0 Mean Offspring/Female: k 15.0 offspring/female ChV

% CV: "*,7.< 40.0 % I06s I (jO (tage 104 of 113 SOP AT14 - Exhibit AT14.1, revision 06-01-11

6S 'of Page 2 of 6 0:T Species: Ceriodaphniadubia CdNaCICR #: _ A_ _

CONTROL Survival andReproduction Data toew Wtg LOT" Replicate number Day 1 2 3 4 5 6 7 .8 9 10

'I Young produced 0 .00 C0 Adult mortality %- L.-j L. I- L _ _--

2 Young produced C 0 0 0 Adult mortality 3 Young produced Adult mortality ___

--0--I_ " _

'--

i I____

1 Young produced - T ,S -r- ION Adult mortality _ ~i--'- ~ j-1_

T Young produced it .. .L % '1._

___a Adult mortality Li .. It-.... .. .. - t_ -V . '_.

6 Young produced "A octi0 T0 Adult mortalityi :

S Younag produced %urvivlI an Rroc tData Total youngproduced 13 zi

S7 3 . 5 17 Final Adult Mortality \.. c: %. ._C-IXfor 3r Broodsn y_ y WZ > ..__.

Note: Adultmortality (L= live, D=dead), SB =split brood (single brood split between two days), CO =carry over (offspring carried over with adult during transfer).

Concentration:

1% Mortality: (5- j Mean Offspring/emale: .tj 600 mg NaCI/L Survival andReproduction Data

_ __ __ __ jReplicate number _

Day _____ 1 2 3 -4 5 16 7 18 9 10 I Young produced -

0

- C)

_ Adult mortality I - _- L I I . l L- L..I I-_

2 Young produced JL" ._____0____ _____

3 Young produced 0~ ]Qý~ c 0 0 U

+/- U-i j.-j I .- t..

Adult mortality __ ' -

T L 4 Young produced [ I b[s 1

_ote: Adult mortality [=iDeS rdii bd itetnw d, ao f 5 db oung produced 1%3 11 - W1s I Autmortality --- \--+/-~ I- __ L__

6 Young produced ii L..___£C) 1.t. .J' In 7 1. Young produced t~ %fr V2... VI +/-.. I-&.. UPi.

Total young produced NIL 30 IS s it) Is 3,5 Z3 z 3IL.

Final Adult Mortality -, U C j16T L. . U Note: Adult mortality (L= live, D = dead), SB = split brood (single brood split between two days), CO carry over (offspring carried over with adult duringw transfer).

Concentration:

% Mortality: o j Mean OffsprinFemale: I

% Reduction from Control: 0.7.

SOP AT14 - Exhibit ATI 4.1, revision 06-01-I1 (tage 105 of 113

Page 3 of 6 SETS Species: Ceriodaphniadubia CdNaCICR#: ____

800 ma NaCIJL Survival and Reproduction.Data .. NewTvtW LO" Replicate number DaY 1 2 3 4 5 6 7 8 9 10 2

IJ I Young produced Adult mortalitI Adult mortality IO in t- II 0)

~ 3_C: . %--

Q Q

[

0 0

L-0L 3" Young produced .Aloi *-- 1-- t-* - i * "_._ .

4 ~npoue mortality 'tJ. *, ,_ L X?' . % 'I_ %_' .'Adult 4 Young produced 'A . "I . I 10 L 1 j*_ j 10 Adult mortality 65 6

Young produced Young produced

[M.c.I C*

K i~~ UT-U

) (*

C) io 010 i 010 0 ( C)

Adult mortality 7 Young produced [ '* ", ' t " i, "

Total young produced bO * , 1 $3!s I Final Adult Mortality %-,,W- C. \-- ý.ii I . .\-...

Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over

. with

...... adult during

transfer).J Concentration:

%Mortality: 0?7.

Mean OffsorinaIFemale: I 3A I

%Reduction from Control: 0. 7

! 1000 mg NaCI/L Survival andReproduction Data.

-j Replicate number _

Day, 1 2 3 4 5 6 7 8 9 10

-l I Young producedd, Adult mortality 0,

0o1 0 010 II_

01 00

,J 2 [ Young produced l 1 0. I 1 0 0 .

Adult mortait y L=JI ' I - ' 1"- I- I U U.

3 4iYoung producedl '-**

Young produced Adult mortality _

O.

1 *1 * *

5[

____ Young produced YonAdult mortality LA..1 1L itŽ ILM 1It ~ lo ~ 11051 ~ I_ __0 1 Young.produced 0 1... /0 0 '

5 Adult mortality L1I A J0 6 Young produced .c.-c- Vb I~ - _

Total young produced "V IL6 I ab 3- a I -' "

Final Adult Mortality " *.. %.-

U. %- . X._. t. .

Note: Adult mortality (L =live, D =dead), SB = split brood (single brood split between two days), CO = carry over (offspring carried over with adult during transfer).

Concentration:

%Mortality: p7.

Mean Offspring/Female: 2k. I I % Reduction from Control: I - CL g I SOP AT14 - Exhibit AT14.1, revision 06-01-11 (tage 106 of 113

"ETS 0

a Page 4 of 6 Species: Ceriodaphniadubia CdNaCICR#: 1____

K1200 mg NaCI/L Survival and Reproduction Data New* q'IT"LOT, Replicate number Day 1 2 3 4 5 6 7[ 8 9 10 Adult mortali t y jj..

2 Young produced j 0 0 Adult mortality . %I- - .- L-31 [Youngproduced 0..J_ 1 1-T.Q.J*.. .1 Adult mortality . ,-

4 Young produced Iz-V[ O I _._. Il' . *1' ft Adult mortality I 4-- ._- j L. . 4_.

1r "

5 Youngproduced ]..

S "i.. , '

Adult mortality __ \..j J'. _1 L J .. [L- ~

6 Young produced C) ) O ~

_[Adult mortality J 4 j'- L [\ik~'- 4 ~ 4 7 jYoung produced 6I ~ I ~ S a.

Total young produced l l J l t I . *

& i 9 Final Adult Mortality *Z . *\ * \* 1 -.. _.

Note: Adult mortality (L = live, D = dead), SB = split brood (single brood split between two days), CO = carry over (offlpring carried over with adult during transfer).

Concentration:

% Mortalit:

Mean Offspring/Female: I 1.S .

I% Reduction from Control: I (A. 2.

.'".3 1400 mg NaCI/L Day 2

I Young

___ Adult mortality Youngproduced Wroduce

__6 1

10 2

L-1 3

k4.

Survival andReproductionData 14 I__

1

-L Replicate number 5

L 0 0 1C 6

L-..

00 1 0 7

I '-

8 9 0) 1

'4-

1) 0i 0 10 Adult mortality U - L '-- j_'-['--I1 ..

3 Yon prdce -)C 0 0 U0 (3 I Adultmortallty 4_. - ý' %_ %- I IL"

_ -,.- 71- t 4 Young produced (on 0 0 C)L 0 .

5 Young Adult produced mortality j 7 0- '-1 '1. - 0It 1 -- 01 I...5 To5a Adult Young mortality produced 'L C)* A I - '1'-I~_--1 2.*L A.f 4 '-

7 Young produced Gi __0 Note: Adult mortality (L = live, D =dead), SB = split brood (single brood split between two days), CO carry over (offspring carried over with adult during transfer).

I Concentration:

% Mortality: 2307 Mean Offspring/Female: 2. 73

1% Reduction from Control: ( n 17. 1 SOP AT14 - Exhibit AT14. I, revision 06-01-11 (tage 107 of 113

0 "ETS0 Verification of CeriodaphniaReproduction Totals

, Environmental Testing Solutions, Inc.

U) Control 1000 mg NaCI/L

- Replicate number Total Day 1 2 3 4 5 6 7 8 9 10 Day Re licate number Total 1 0 0 0 0 0 0 0 0 0 0 0 1 2 3 4 5 6 7 8 9 10 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 6 4 5. 5 4 5 3 44 4 4 5 4 4 3 3 3 4 4 3 37 5 12 11 12 13 12 12 12 11 13 112 120 5 10 11 12 10 10 10 13 10 ai 11 108 6 0 0 0 0 0 0. 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 7 17 15 15 18 16 15 15 15 15 17 158 7 16 14 16 16 13 16 14 17 17 13 152 Total 33 30 31 37 32 32 32 30 33 32 322 Total 30 30 330 0 26 29 30 31 32 27 297 600 ng aCI/L 1200 mg NaCI/L Replicate number Total Day 1 2 3 1 4 5 6 7 8 9[Tt Day Re plicate number Total 1Y 2 3 14 5 6 1 7 8 9 10

- 0 0 0 0 0 0 0 0 0 0 0 1 0 0- 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 23 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0.

4 5 3 5 5 4 4 5 4 4 4 43 4 2 4 3 3 4 4 3 4 2 2 31 5 12 10 13 13 11 14 12 12 12 12 121 5 10 6 5 8 5 9 .4 4 6 3 60 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0. 0 0 7 15 18 17 15 15 17 16 17 13 16 159 7 2 6 0 0 3 5 2 5 1 0 24 Total 32 31 35 33 30 35 33 33 *29 32 323 Total 14 16 8 11 12 18 9 13 9 5 115 800 Mg NaCIJL 1400 mg NaCIIL Re licate number Replicate number Total 1 2 3 4 5 6 7 8 910 Day 1 2 3 4 5 6 7 8 9 10

- 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 4 0 2 0 0 0 0 0 0 0 0 3 4 4 3 4 5 4 5 5 4 4 4 42 5 11 13 13 12 12 10 12 13 10 10 116 5 2 0 1 4 1 0 2 1 3 3 17 6 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0. 0 2 0 0 0 0 2 7 15 19 15 17 16 15 1 14 17 17 163 7 0 1 0 00 0 0 0 0 1 Total 30 35 32 34 32 30 35 31 31 31 321 Total 2 3 1 4 1 3 2 1 3 3 23 File: CdNaCICR_050713new-ywt.xlsx Entered by: J. Sumner Reviewed by: d

(0 0 E Ceriodaphniadubia Chronic Reference Toxicant Test EPA-821-R-02-013, Method 1002.0 0' Environmental Testing Solutions, Inc.

Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: CdNaCICR #148 Test dates: May 07-14, 2013 Concentration Replicate number Survival Average reproduction Coefficient of Percent reduction from (mg/L NaCI) 1 2 3 4 5 6 7 8 9 10 (%) (offspring/female) variation (OA) control (%)

Control 33 30 31 37 32 32 32 30 33 32 100 32.2 6.2 Not applicable 600 32 31 35 33 30 35 33 33 29 32 100 32.3 6.0 -0.3 800 30 35 32 34 32 30 35 31 31 31 100 32.1 6.0 0.3 1000 30 30 32 30 26 29 30 31 32 27 100 29.7 6.6 7.8 1200 14 16 8 11 12 18 9 13 9 5 100 11.5 34.1 64.3 1400 2 3 1 4 1 3 2 1 3 3 100 2.3 46.1 92.9 Dunnett's MSD value: 2.351 MSD = Minimum Significant Difference PMSD: 7.3 PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test Lower PMSD bound determined by USEPA (I 0 thpercentile) = 13%.

Upper PMSD bound determined by USEPA (90th percentile) = 47%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001 b).

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-004 and EPA-821-B-01-005.

US Environmental Protection Agency, Cincinnati, OH.

File: CdNaClCR_050713new-ywt.xlsx Table populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Spreadsheet entered by: J. .Sumner Reviewed by: -

eETýS SEnvlron-mtal Testng Solutions. Inc.

Statistical Analyses Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/7/2013 Test ID: CdNaCICR Sample ID: REF-ReffToxcant End Date: 5114/2013 Lab ID: ETS-Envir. Testing Sol. Sample Type: NACL-Sodium chloride Sample Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Cerlodaphnia dubia Comments:

Conc-mglL 1 2 3 4 5 6 7 8 9 10 D-Control 33.000 30.000 31.000 37.000 32.000 32.000 32.000 30.000 33.000 32.000 600 32.000 31.000 35.000 33.000 30.000 35.000 33.000 33.000 29.000 32.000 800 30.000 35.000 32.000 34.000 32.000 30.000 35.000 31.000 31.000 31.000 1000 30.000 30.000 32.000 30.000 26.000 29.000 30.000 31.000 32.000 27.000 1200 14.000 16.000 8.000 11.000 12.000 18.000 9.000 13.000 9.000 5.000 1400 2.000 3.000 1.000 4.000 1.000 3.000 2.000 1.000 3.000 3.000 Transform: Untransformed Rank i-Tailed isotonic Conc-mg/LL Mean N-Mean Mean Min Max CV% N Sum Critical Mean N-Mean D-Control 32.200 1.0000 32.200 30.000 37.000 6.177 10 32.250 1.0000 600 32.300 1.0031 32.300 29.000 35.000 6.026 10 110.50 75.00 32.250 1.0000 800 32.100 0.9969 32.100 30.000 35.000 5.956 10 101.50 75.00 32.100 0.9953 11000 29.700 0.9224 29.700 26.000 32.000 6.554 10 71.50 75.00 29.700 0.9209

1200 11.500 0.3571 11.500 5.000 18.000 34.112 10 55.00 75.00 11.500 0.3566

1400 2.300 0.0714 2.300 1.000 4.000 46.059 10 55.00 75.00 2.300 0.0713 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.97812 1.035 0.17037 1.33902 Bartlett's Test indicates unequal variances (p = 8.24E-03) 15.5538 15.0863 Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU Steel's Many-One Rank Test 800 1000 894.427 Treatments vs D-Control Linear Interpolation (200 Resamples)

Point mglL SD 95% CL Skew IC05 921.875 64.1655 787.455 1002.89 -1.6172 IC10 1007.42 21.4035 936.218 1020.21 -2.6078 IC15 1025.14 7.97486 1006.95 1037.62 -0.6579 1.0.

IC20 1042.86 7.40439 1026.9 1055.34 -0.3184 0.9 IC25 1060.58 7.23559 1045.83 1072.68 -0.1999 IC40 1113.74 8.15735 1098.11 1128.27 -0.0270 0.8.

ICS0 1149.18 9.69551 1130.03 1169.33 0.0275 0.7:

0.6.

o*0.5-1 0.4 g 0.3 0.2 0.1 0.0

-0.1 . . . . I 0 500 1000 1500 Dose mg/l Dose-Response Plot 40 35 30 0 25 20 15 10 5

0_

8 B File: CdNaCICR_0507I3new-ywt.xlsx Entered by: J. Su iner Reviewed by:

(age 110 of 1136

0 Statistical Analyses

.ETS

( " Environmental Testing Solutions, Inc.

Used for PMSD calculafion only.

Ceriodaphnia Survival and Reproduction Test-Reproduction Start Date: 5/7/2013 Test ID: CdNaCICR Sample ID: REF-Ref Toxicant End Date: 5/14/2013 Lab ID: ETS-Envir, Testing Sol. Sample Type: NACL-Sodium chloride SampJe Date: Protocol: FWCHR-EPA-821-R-02-013 Test Species: CDMCeriodaphnia dubia Comments:

Conc-mg/L 1 2 3 4 5 6 7 8 9 10 D-Control 33.000 30.000 31.000 37.000 32.000 32.000 32.000 30.000 33.000 32.000 600 32.000 31.000 35.000 33.000 30.000 35.000 33.000 33.000 29.000 32.000 800 30.000 35.000 32.000 34.000, 32.000 30.000 35.000 31.000 31.000. 31.000 1000 30.000 30.000 32.000 30.000 26.000 29.000 30.000 31.000 32.000 27.000 1200 14.000 16.000 8.000 11.000 12.000 18.000 9.000 13.000 9.000 5.000 1400 2.000 3.000 1.000 4.000 1.000 3.000 2.000 1.000 3.000 3.000 Transform: Untransformed 1-Tailed Conc-mg/L Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD D-Control 32.200 1.0000 32.200 30.000 37.000 6.177 10 600 32.300 1.0031 32.300 29.000 35.000 6.026 10 -0.097 2.287 2.351 800 32.100 0.9969 32.100 30.000 35.000 5.956 10 0.097 2.287 2.351

1000 29.700 0.9224 29.700 26.000 32.000 6.554 10 2.432 2.287 2.351

'1200 11.500 0.3571 11.500 5.000 18.000 34.112 10 20.137 2.287 2.351

1400 2.300 0.0714 2.300 1.000 4.000 46.059 10 29.087 2.287 2.351 Auxiliary Tests Statistic Critical Skew Kurt Kolmogorov D Test indicates normal distribution (p > 0.01) 0.97812 1.035 0.17037 1.33902 Bartlett's Test indicates unequal variances (p = 8.24E-03) 15.5538 15.0863 Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob df Dunnett's Test 800 ,1000 894.427 2.35056 0.073 1717.67 5.28333 5.3E-39 5, 54 Treatments vs D-Control File: CdNaCICR_050713new-ywt.xlsx Entered by: J. Sumner Reviewed by:

tage 111 of 113

Page 5 of 6 0

0 Eo n~d Species: Ceriodaphniadubia CdNaCICR #: __8 Daily Chemistry:

Concentration Parameter Conductivity o (Aimhos/cm)31 CONTROL *AUInainty (mg CaCOsiL)ID4S0

Hardness (mg CaCO3IL)4M

__________*Temperature (00) UfA v.DvA pH (S.U.)

-F--

1_ 1.93 79S -7".4 I 7'C DO (mg/L) -7. 7 IIAA I 600 mg NaCi/L Conductivity F 1330 m1 (Iimh m)

Temperature (OC) Ls !Cj lq.g 11 1-4 pH (S.U.) S 6.o0 II -1.Z I -7.s II-7.3 1 7.41 DO (mg/L) 1~ 11 IG F '16 II 800 mg NaCI/L Conductivity (Rimh stem) __ 1710 1730 4 ",.C1

Temperature (*C) *I.'i Ti -I 11 II "I.sIIE Y 'U pH (S.U.)

- " " 4 4o0---

I.n11"7- I.*q II-1Z "7.97 DO (mg/L) 7.C 7.7 1000 mg NaCI/L Conductivity Z 1 z.sc...

(mhosm 2040_U 0ooiz

Temperature (0C) "LN .A *.. eI "1.4.

bl.E II l.,*%-

PH (S.U.) JIMq A- q 7.115 DO (mg/L) 1." 1.., O 7.ett .7 1200 mg NaCIIL Conductivity (0 3I340 Zohs/m Z4`40L 2430 L3

,__________,___. *Temperature CC).

pH (S.U.)

- - - I- - -- __________ F DO (mg/L) 7.7 1400 mg NaCI/L Conductivity (1nmhos/cm)

Temperature (00 STOCK Conductivity id oco Initial ii lnai i initial ii rJnal i iniiual ii lnal I

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and hardness performed by the analyst identified on the bench sheet specific fo each analysis and transcribed to this bench sheet by:

SOP AT14 - Exhibit AT14.1, revision 06-01-11 age 112 of 113

.Page 6 of 6 0:T 6

Species: Ceriodaiphniadubia CdNaCICR #M IML rteb %Iv-T LOT Day (Analyst identified for each day perfbrmed pH, D.O. and conductivity measurements only.)

3 4 5 6

_ _ _Analyst K_- Lm

10. 11 UB 1 1 Concentration Parameter L pH (S.U.) e)46 9.0c 11o

. 9 6..q DO (mg/L) I It 04e s.I I.s - 7.7 7 Conductivity (gtmhos/cm) at 2 . 37 CONTROL *Alkalinity lot (mg CaCOA/) 6

Hardness (mg CaCOA/)

Temperature 1 1O L.i PH (S.U.) " ( O0. 004 .0oo 6.03 7.93 7." 1.90 DO (mgIL) 82. S.9 7._

600mg NaCl/L Conductivity

Temperature 13(00

-LA 1trio n 1 LA*a US.0 "L-A "*LA.I6 ..

t .0 PH (S.U.) .4 0

-DO(mg/L) A 02.1 ?It 1 7.7-800 mg NaCI/L Conductivity l~)IO (Amuhos/cm) GUM____8__

Tmeaue -L. 'O.l -LA- I-- - -- q pH (S.U.)

F

"+.q- Ii I e~~ 7.44 .flz 7. &r 7.411

-4 11

9) 71: I DO (mrg/L) l

-.t W1-1 7.7-,

1000 mg NaCI/L Conductivity

Tnmhospcmar z 100 L4.e+-;

Temperature OCt) S U - Ih II'L"*j I

- a *Lq.i- i11 U- I Vi.g 1 -L-A.71-

~ 1 -6.0 am 11 '- I pH (S.U.) I G.OL 16 7qg 9.02 1 ___ 7.41 11.W A-- T -F - 41 I DO (mg/L) 1200 mg NaCI/L Conductivity 0'2, Wo w Z540 7 (umhos/cm)

Temperatu~re 5 IK '.IqLq'oJ

'iA-Iqu t. 1 'Il ZSLq.o (00 ..

pH (S.U.) /k,.* *,Ž0 ,O3 7, 7 __.Ot "¶, J+/-, 7 .. 11 7 el 84 1400 mg NaCI/L DO (mg/L)

Conductivity 1 1 9,3 e.0 ILI ..1 783.

-L-'q.#

(0Q

Temperature -24.9 '*".' [ ,"*

Initial Final Initial I Final I Initial

~

Final I

Initial Final a

I - H ii

Temperatures performed at the time of test initiation, renewal or termination by the analyst identified in the Daily Renewal Information table located on Page 1. Alkalinity and berdness performed by the analyst identified on the bench sheet specific for each analysis and transcribed to this bench sheet by:..

t age 113 of 113 SOP AT14 - Exhibit ATI4.1, revision 06-01-11

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