ML022760298
| ML022760298 | |
| Person / Time | |
|---|---|
| Site: | Sequoyah  |
| Issue date: | 09/13/2002 |
| From: | Nida D Tennessee Valley Authority |
| To: | Office of Nuclear Reactor Regulation, State of TN, Dept of Environment & Conservation |
| References | |
| Download: ML022760298 (116) | |
Text
Tennessee Valley Authonty, Post Office Box 2000. Soddy-Daisy, Tennessee 37379-2000 September 13, 2002 State of Tennessee Department of Environment and Conservation Division of Water Pollution Control Enforcement & Compliance Section 6th Floor, L & C Annex 401 Church Street Nashville, Tennessee 37243-1534 Attention: Ms. Evelyn Haskin
Dear Ms. Haskin:
TENNESSEE VALLEY AUTHORITY - DISCHARGE MONITORING REPORT FOR SEQUOYAH NUCLEAR PLANT Please find enclosed the Discharge Monitoring Report (DMR) of August 2002 for Sequoyah. Please contact me at (423) 843-6700 if you have any questions or comments.
Sincerely, Diedre B. Nida Environmental Supervisor Signatory Authority for Richard T. Purcell Vice President Sequoyah Nuclear Plant Enclosure cc (Enclosure):
Chattanooga Environmental Assistance Center Division of Water Pollution Control State Office Building, Suite 550 540 McCallie Avenue Chattanooga, Tennessee 37402-2013 U.S. Nuclear Regulatory Commission ATTN: Document Control Desk Washington, D.C. 20555 Pr1ntd on mcded peaW
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONrrORING REPORT (DMR)
Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No 2040-0004 TNO0245 01 l F - FINAL SODDY - DAISY TN 37384 GiG DIFFUSER DISCHARGE PERMIT NUMBER IDISCHARGE NUMBT FaclIty_ ]___*- SFQ..AHNUCLEARf LAN MONITMPlNG PFI*nQI' EFFLUENT YFArg Mo nAV I YFARI I MO rnAY ATTN: Dledre B.Nida From 02 1 08 01 I To 10 1 OR l l NO DISCHARGE [ZI NOTE' Read Instructions before completing this form I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system designed to assure that qualified personnel properly gather and evaluate the infortnation subrn'tted Based on my inquiry of the person or persons who manage the system, or those persons directly responsible for gathenng the nformation, the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. I am aware that there are significant penalties for submnting false information, rncluding the possibility of fine and Impnsonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference allattachments here)
No closed mode operation. The following injections occured: 1. PCL-222 (max. calc. conc. was 0.055mg/L--limit O.100mg/L) 2. CL-363 (max. cale. conc. was 0 009mg/L--hjmt 0.100mg/L) 3. PCL 222/PCL-401 (max. cafc. conc. was 0 019mg/L--limit 0.100mg/L) 4. H-130M (max. calc. cone was 0.025mg/L--Ilmit 0 050mg/L)
EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 2
PERMITTEE NAME/ADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR)
Name TVA - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMB No 2040-0004 Address BOX 2000
_ O E ~ l .I . ... .T.FO . .-. .. TN06450O110 F - FINAL
_ 5_. - SE5YUM_ NUL_.A . . . . . . PERMIT NUMBER Fm DISCHARGE NUMBER DIFFUSER DISCHARGE V ._~kv.
-- _QHNCE~ LN MC)NITOP*ING PFRIQQC EFFLUENT k.atlonD-eHAMrI e QV _BQ. *d .......... VFArP IMc) /rAY_ 1 YAr I Mn I AV ATTN: Diedre B. Nlda From 02 08 01 1 To 102 108 11 NO DISCHARGE I I ...
NOTE* Read Instructions before completing this form I
COMMENTS AND EXPLANATION OF ANY CCW data for August 2002 is attached 3320-1 (REV 3/99)
(REV 3/99) editions maybe Previous editions used Page2of 2 EPA Form 3320-1 EPA Form Prewous may be used Page 2 of 2
CCW Data for August 2002 CCW CHANNEL DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mag/L)
August 19, 2002 <05 CCW TRENCH DISCHARGE DATE EXTRACTABLE PETROLEUM HYDROCARBONS COMMENTS (mq/L)
August 19, 2002 1.2 Page 1
PERMITTEE NAME/ADDRESS (Include Facilty Name.aocationifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (OMR) (SUBR 01)
No~me TVA - SEQUOYAH NUCLEAR PLANT OMB No 2040-0004 Address BOX 2000 ITN0026450 F 101 T ý FF-FINAL SODDY - DAISY TN 37384 IPERMIT NUMBER I DISCHARGE NIMBR BIOMONITORING FOR OUTFALL 101
_Fgllty_ _TV__A --SF MC)NIT(PRINC- PFRI*rD 1 EFFLUENT From 102 r FoAP Mm 08 13AY 01 To 02 0 3A ** NO DISCHARGE F- ...
ATTN' Dledre B Nida NOTE, Read instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE I EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS IC25 STATRE 7DAY CHR SAMPLE >100 (23) 0 1/90 COMPOS CERIODAPHNIA MEASUREMENT 2)
TRP3B 1 0 0 4PERMIT:~
4REQUIREMENT ~ 7~43ee*e*T19T
. PERCENIT' ,QUART-~ COMPOS EFFLUENT GROSS VALUE ~
2~yV MNMMERLY. '~
IC25 STATRE 7DAY CHR SAMPLE >100 (23) 0 1 / 90 COMPOS PIMEPHALES MEASUREMENT TRP6C 1 0 0 PERMIT 2K- "49 4> PERCENT <,QUART-V COMPOS EFFLUENT GROSS VALUE ~ RQ ' ,'4 <ERLY<
SAMPLE MEASUREMENT MEASUREMENT' SAMPLE MEASUREMENT SAMPLE MEASUREMENT MEASUREMENT MREASUREMENT SAMPLE MEASUREMENT S,....o..,.,,.-.-.,,.- -... 0o ==o 0,,4
<PERMIT .
4 '~4
' dietono 44,up 'so 4Inaccrdnc 4 '2>'44444$ *t -. syte deine o sur ha u4edprsne \4*$4 4 <4 41
.REC0ULREM1ENT~;"~4~~'~~~A~'&~"~42 SAM1PLE MEASURPEMENIT Richard T. Purcell properly gather and evaluate the Information submitted Based on my Inquiry of the person or persons who mranage the systemn, or those persons Environmental Supervisor information, the Information submitted Is, to the bestdirectly responsibleand of my knowledge for belief, gathering true,theaccurate, 423 843-6700 02 09 13 Site Vice President and complete I amaware that there are significant penalties for submitting false Information, SIGNATURE OF PRINCIPAL EXECUTIVE RPITDincluding TYE the possibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREA NUMBER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oll attachments here)
Toxiroty was sampled from August 4,2002 through August 9,2002. Report Is attached.
EPA Form 3320-1 (REV 3/99) Previous editons may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FactlifyNamelLocationif Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01)
Address BOX 2000 S. .. NER.OE-CES ,$..-2A- TN0026450 L Z 101 Y F-FINAL oDODY-..
-- __ TN3_.A - _
M PERMIT NUMBER IDISCHARGE NUMBER ANNUAL MONITORING (PCBS) fI ._]V.A - SEQQQYAHLNCL ELARULj ._ ....
Lc_..tLI._n _HMjQ0N_.C_,QU N_! _. .
/IF"AC\I iITtC3fl11NI WLzLx1.LLa tŽ DE nit rw4 LmLlll"Ld
-I I
EFFLUENT 1111kit-FromI 0I9II 1I Tolo2L - 08 31 *** NO DISCHARGE AITN: Diedre B. Nfda NOTE. Read instructions before completinq this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS AVERAGE MAXIMUM POLYCHILORINATED BIPHENYLS SAMPLE ******** ******** ******** 0.0 (19) 0 1 /365 GRAB MEASUREMENT 39521 1 0 0 PERMIT -,- -. ** **, M*G/ L,,****.-, ANNUAL, 'GRAB REQUIREMENT, EFFLUENT GROSS VALUE .~~< 7Y SAMPLE MEASUREMENT PERMIT REQUIREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE
~REQUIREMENT~~
MEASUREMENT SAMPLE MEASUREMENT
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SAMPLE MEASUREMENT
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- direction or supervision In accordance with a aystem dealgned to assure that qualified personnel ~I
~ ~Richard EPA ~~~~~~~RýQIEI Fom32.IRV39)
T. Purcell Peiu dfins properly aTeue gather and evaluate the Information subirrtted Based on my Inquiry of the person orI ae1o persons who manage the system, or those persons directly responsible for gathering the EniomnIlSprio d complete I em aware that there are signIficant penalties for submitting false Information, aneVcePeidn SIGNATURE OF PRINCIPAL EXECUTIVE JI Site resident Vice nfurmatonthe Ino rmab t onfi bne tted Impstonthenbet ofo my know atinds e~f re cuae ledgevo vrn e lS prio 2 4-7 0 0 9 1 TYPED OR PRINTED i lu ngteps bityofiea d rrno m n fo kn w gvoat nsOFFICER OR AUTHORIZED AGENT AREA NM BER YEAR MO DAY COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Rleferenceaollattachmentshere)
EPA Form 3320-1 (REV 3199) Previous editions may be used Page 1 of I
PERMITTEE NAMEIADDRESS (Include FacilityName/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved ni'er-cie= nIIrIMITr0iIM15 morI=DIT ffnMR)
STV._A - SEQUOYAH NUCLEAR PLANT (SUBR 01) OMBI No. 2040-0004 Addregj ... QXE NTER*fFI_USA
.. A... .. 0026450 103 G F-FINAL
._ODDY:AISY_
- TN 37384 ....... PERMITNUMBER IDISCHARGE NUMBER LOW VOL. WASTE TREATMENT POND KMn"\NITr)DINM( PIPDIfl' I EFFLUENT LocpIt1-nHNQN-CQ M ... ...
To MO21813 ...NO DISCHARGE = ...
ATTN: Dledre B. Nlda From 102 i 08 101 NOTE: Read Instructions before completina this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE 7.2 8.9 0 13/31 GRAB MEASUREMENT 78 (12) 00400 1 0 0 CPEMT,.
- iREQUIREMENT;***:=-***,! ,,*'*'> ; ,"i'* 6.
?ou;÷h , *'MAXIM*9.SIX ,~ SU THREE/ ~GRAB~
EFFLUENT GROSS VALUE i****k,*~ 0 0 ~ S WEI~~*****~~
SOLIDS, TOTAL SUSPENDED SAMPLE 121 164 (26) 12 15 (19) 0 4/31 GRAB MEASUREMENT 00530 1 0 0 -PERMIT,-'<, ;~8iMID ~ 125O 1,,ý, LBSIDY -,,,100i MG/L .WEEKLY, 'GRAB EFFLUENT GROSS VALUE .Ž,Z" .MO AVG, DAILYIXi MX - ,*MOýAVG DAILY.MX' I . :§!
OIL AND GREASE SAMPLE <52 <55 (26) <5 <5 (19) 0 4 /31 GRAB MEASUREMENT(2)19 190 25*
.250 LBS/DY < **7 1-w>15** MG/L WEEKLY 'GRAB*
00556 1 0 EFFLUENT GROSS VALUE 0 ' PERMITn REQUIREMENT- 0i 1 ,* LB , , D,
____________________M0'AVG DAILY- MXK M AVG; ~ DALY-MX4 I____'
FLOW, IN CONDUIT OR THRU SAMPLE 1.212 1.360 (03) 0 31 / 31 TOTAL TREATMENT PLANT MEASUREMENT 50050 1 0 0 P;ERMITZTL . ." e. 'DAILY, :TOTAL.
SAMPLE MEASUREMENT SAMPLE MEASUREMENT SAMPLE MEASUREMENT TYPED R PRIPERMITD NAME/TITLE PRINCIPAL EXECuTIVE OFFICER *r`y und*er penalty of law that this dccur~ntn and all Snachnmenls were prepared under mny Iel J ) _* _ TELEPHONE DATE direction or aupeivision in accordance w~tha system designed to assure that qualified personnel Richard T. Purcell prfoperty gather and evaluate the information subrrtted Based on my tnquiry of the person or persona who manage the system, or those persons directly responsible for galhenng the Environmental Supervisor Site Vice President and conplete I em aware that there are significant penalties for subnsttmng fatse Infoffn5tion, SIGNATURE OF PRINCIPAL EXECUTIVE ........ L .
TYE RPRNE ncluding the possiblity oftine and tmprisonment for knowlng violations OFFICER OR AUTHORIZED AGENT ARA NU.JiMBER YEAR MO DAY EPAar For COMMENTS AND EXPLANATIONPu320llOF ANY rvousediton VIOLATIONSprV39mayber used udeferenceo nomto ttachments urve all here) ae nm nur esno of Pageat 1h 1h EPA Form 3320-1 (REV 3/99) Previous editionsmay be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include FacilityName!Location ifDifferent) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR) (SUBR 01)
Nome TA - SEQUOYAH NUCLEAR PLANT OMB No. 2040-0004 AddrefQ_ ._BO..*O0fl .... 1 107 G I TN0026450 F-FINAL SODDY - DAISY TN 37384 PERMIT NUMBER IIDISCHARGE NJUME METAL CLEANING WASTE POND LoAc.-tTLo: HedJeBN ......
. EFFLUENT KAr)MITn1D1M(" PI0DIr'if I Irom-AP01 08101 I To 02 08 31 - NO DISCHARGE XX A'nTN: Diedre B Nlda NOTE' Read instructions before completing this form PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO. FREQUENCY SAMPLE EX OF TYPE AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS ANALYSIS PH SAMPLE ***** ********
MEASUREMENT (12) 00400 1 0 0 ***PERMIT***** ***** ****** 9.0****
REQUIREMENT (S9)L' EFFLUENT GROSS VALUE PHOSPHORUS, TOTAL (AS P) SAMPLE ***** ***** ** ******** (19)
MEASUREMENT (19) 00665 1 0 0 77-PERMIT~r* ;,7****,P *-**,;, 44 MG,-L , DAILY: COMPOS EFFLUENT GROSS VALUE EUREMENT SOLIDS, TOTAL SUSPENDED SAMPLE * ****4 MEASUREMENT (19) 0053 1 0 0 PRMIT 36 G/LDAILY-, COMPOS EFFLUENT GROSS VALUE R..U..E.EN.... .......... s ___..._
OIL AND GREASE SAMPLE ******** ******** ****
MEASUREMENT . (19) 00556 00 1
1 30 0 002PERMI i'PERMITý T -*** " Vih ';,****
- ,. ' h'****"*!;' ~ ~ ****
44 *:A! ***-***.'*: :Ail ':;*4*4*4*4*,:; 3**"
5> MG/L:...
MGIL .... -, .. ....
M.IY, ...
GRAB~
EFFLUENT GROSS VALUE ,-...-,______"_ .. L DAILY ____""___ ___....__ 'W COPPER, TOTAL (AS CU) SAMPLE ***** ****
MEASUREMENT (19) 01042 1 0 0 PERMiT **i 7.......
- 7' *G *** ****; MGILL***-*,**
- DAIL...COMPOS DY*,*
EFFLUENT GROSS VALUE RQIEET ________
IRON, TOTAL (AS FE) SAMPLE 4***4~D ILM-___________
MEASUREMENT *(9 01045 1 0 0 ,~~PIRMITiý J1 4** ***44ý MGfL ~DAILY- COMPOS:
EFFLUENT GROSS VALUE RUIEET KK
________________ __________ DAILY, MX&
__________ C ___ ___
FLOW, IN CONDUIT OR THRU SAMPLE (03)* **44 ****
TREATMENT PLANT MEASUREMENT (3 50050 1 0 0 REPORT *REPORT- MGD .... ý,DAILY :CALCTDs EFFLUENT GROSS VALUE MOEUIEET~IP AVGI~ DFAIVIV
ýJby i-i~____ ___
NAME/TITTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my drection or superavson in accordance with a system designed to assure that qualified personnel I. g &/,1 T TELEPHONE DATE
- Richard T. Purcell property gather and evaluate the Information submrtted Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the 1 Environmental Supervisor I
information, the information submitted Is , to the best of my knowledge and belief, true. accurate. 423 843-6700 02 09 13 Site Vice President and complete I am aware that there are significant penalties for subrrutting false Information, SIGNATURE OF PRINCIPAL EXECUTIVE I TYPEDORPRINTED including the possibility of fine and Imprisonment for knowing violations OFFICER OR AUTHORIZED AGENT AREAI NUMBER YEAR MO DAY TYPED OR PRINTED _______________________________ _________________
COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
No phosphate bearing chemicals were employed. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions maybe used Pagel1 of 1
PERMITTEE NAME/ADDRESS (Include FacilityNameALocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved N__t-. TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR) OMB No 2040-0004 (SUBR 01)
Addres_. __O209 flNTEORCFFIQ S..2A.
TN0026450 1 110 G FF-FINAL
_ _7.M
-Q U __ ..
Dg _W
_.*& . .. . .T.'_ PERMIT NUMBER IDISCHARGE NUMER RECYCLED COOLING WATER Lozoation__[-MIRTQN_ ' KAtKlITntDIKMr-- DIDcrn EFFLUENT A . .... 1.11 ..4 From 102 FroAm 08 Min 01 1 Tol102 TAo FAP 08 131 MO DAY NO DISCHARGE * ...
ATTN. Diedre B. Nida NOTE Read Instructions before completing this I I
I Certify under penalty of law that this document and all attachments were prepared under my direction or supervision Inaccordance with a system designed to assure that qualified personnel properly gather and evaluate the information submrtted Based on my Inquiry of the person or persons who manage the system, or those persons directly responsible for gathering the information, the Information submitted Is , to the best of my knowledge and belief, true, accurate, and complete I am aware that there are significant penalties for submitting false Information, ncluding the possibility of fine and Impnsonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
No Discharge this Period EPA Form 3320-1 (REV 3/99) Previouseditions may be used Page 1 of I
PERMITTEE NAME/ADDRESS (Include FacilitY Name/Location if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved
_Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No 2040-0004 Address .Q*X.. TN0026450 I 16 G FFIA flNTEROFFIC. -2A ... .. . PRUMBReI F -FINAL Facilit
___ TN 37384
__*.p.y-DAIS JVA -SE-QQYU._LArLANT M F 7PERITNMBE DICHA GE G BACKWASH Location HA__MjLTNCO _.._O......'
Nl MFNITromIN PF" EFFLUENT YFAR I M Ifr)AY I IYFAy MQ* I nay ATTN: Diedre B Nida From 02108101 1 T0102108131 .** NO DISCHARGE LIZ NOTE Read instructions before completinq this form COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference oil offochmenfs here)
Operations is required once per shift to perform visual inspections.
i-re viotis ecirions may cc usec Page 1 of 1 EPA F-orm 3320g-1 (REV 3S/99), Previous editions may be used Page 1 of 1
PERMr-rEE NAME/ADDRESS (Include Facility NamelLocation if Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)
MAJOR Form Approved Name TVA - SEQUOYAH NUCLEAR PLANT DISCHARGE MONITORING REPORT (DMR)
(SUBR 01) OMB No 2040-0( 004 Address ._ 202M -- ---
- - - ._.NTELOf~ Fa, *2Aý . . ._ . . TN06450O111 F- FINAL SODDY - DAISY TNU_*L.RPANr 3734 _ . PERMIT NUMBER IDISCHARGE NTMoR BACKWASH FD.*.:___ -M - SQQUOY,.M-..N_
I M4QNIT('fINC- PFRIn EFFLUENT Location- I-Mj.,QM_._._* Q3JNI .... .y .... YrA.,P(:.._* I MoIrA I rrP mn I r)AY From l02108101 1 To L021I08 1 311 **.NO DISCHARGE D ...
AITN: Diedre B. Nida NOTE Read instructions before completinq this form.
Certify under penalty of law that this document and all atltachments were prepared under my Irectlon or supervision In accordance with a system designed to assure that qualified personnel 3roperdy gather and evaluate the information submsted Based on my inquiry of the person or 3ersons who manage the system, or those persons directly responsible for gathering the nformation, the Information submrled Is, to the best of my knowledge and belief, true, accurate, snd complete I am aware that there are significant penalties for submitting false Information, i.,
ncluding the possibility of fine and imprisonment for knowing violations COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachmentshere)
Operations is required once per shift to perform visual Inspections.
crv rrcvruus rruiriuns may ye useo Page 1 of 1
,320-1U-flrsv rurm ,3.,eu-I tr-'/- r'orm iinl=V ,xIg r'reviou5editions may be used Page 1 of 1
PERMITTEE NAME/ADDRESS (Include FaculhtvNama/Locationif Different) NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES) MAJOR Form Approved DISCHARGE MONITORING REPORT (DMR) (SUBR 01) OMB No. 2040-0004 Name WA - SEQUOYAH NUCLEAR PLANT Address BOX 2 . .. . TN0026450 __118 G I F- FINAL
--- _ QflwwmaLT',O - -. - -- - -_-
__ __ __ SODDDY -_..&IIS_ __ TN_.73ý84 . . . . . . . PERMIT NUMBER , DISCHARGE NUMBER WASTEWATER & STORM WATER Di._r LoatTon_H: N *.d*o .. ._ KA iMIT-DI\I-'* PiPil'n I EFFLUENT From A0 I 0 I 0Y1T ol02 08131I .. NO DISCHARGE [ "'
ATTN: Dledre B. Nlda NOTE Read Instructions before completing this form COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference a/l attachmentshere)
During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall. No Discharge this Period EPA Form 3320-1 (REV 3/99) Previous editions maybe used Page 1 of 1
September 9, 2002 Diedre B. Nida, SB 2A-SQN SEQUOYAH NUCLEAR PLANT (SQN) TOXICITY BIOMONITORING, NPDES PERMIT NO. TN0026450, COMPLIANCE TOXICITY TESTS, AUGUST, 2002 Attached are two copies of the subject report for submission to the state of Tennessee and a copy of the report is included for your records. The report provides results of compliance testing using fathead minnows and daphnids.
Outfall 101 samples collected August 4-9, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
Call me at (256) 386-2755 if you have any questions or comments following yo ur review of the report.
Cynthia L. Russell Biologist Environmental Engineering Services- West CEB 3A-M Attachment cc (Attachment):
Files, ER&TA, CEB 1B-M SQN August 2002m
TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT INTRODUCTION / EXECUTIVE
SUMMARY
Report Date: September 9, 2002
- 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA
- 2. County / State: Hamilton / Tennessee
- 3. NPDES Permit #: TN 0026450
- 4. Type of Facility: Nuclear-Fueled Electric Generating Plant
- 5. Design Flow (MGD):
- 7. 1Q10: 2,992.4
- 8. Outfall Tested: 101
- 9. Dates Sampled: August 4-9. 2002
- 10. Average Flow on Days Sampled (MGD): 1692, 1687, 1662
- 11. Pertinent Site Conditions: No unusual conditions reported. (Production/
operation data will be provided upon request).
- 12. Test Dates: August 6-13, 2002
- 13. Test Type: Short-term Chronic Definitive
- 14. Test Species: Fathead Minnows (Pimephalespromelas)
Daphnids (Ceriodaphniadubia)
- 15. Co ncentrations Tested (%): Outfall 101: 10.98, 22. 43.9, 72. 100 Intake: 100 Pimephalespromelas: UV treated Outfall 101: 10.98, 22, 43.9, 72, 100 UV treated Intake: 100
- 16. Permit Limit Endpoint(%): Outfall I01: IC25 = 43.9%
- 17. Test Results: Outfall 101: Pimephalespromelas:IC 5 > 100 Ceriodaphniadubia: IC25 > 100 UV treated Outfall 101: Pimephalespromelas:ICK > 100
1,
- 18. Facility
Contact:
Diedre B. Nida Phone #: -(423) 843-6700
- 19. Consulting / Testing Lab: Environmental Testing Solutions, LLC
- 20. Lab
Contact:
Jim Sumner Phone #: (828) 862-8193
- 21. TVA
Contact:
Cynthia L. Russell Phone #: (256) 386-2755
- 22) Notes: Outfall 101 samples collected August 4-9, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC values, for both 25 species, were
> 100 percent. Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
2
METHODS
SUMMARY
Samples:
- 1. Sampling Point: Outfall 101, Intake
- 2. Sample Type: Composite
- 3. Sample Information:
Date Date Date (MM/DD/YY)/ (MM/DD/YY)/ Arrival Initial (MMIDD/YY)/
Sample Time (EST) Time (EST) Temp. TRC* Time (EST)
ID Collected Received (°C) (mg/L) Used 101 08/04/02 1244 to 08/06/02 1040 0.7t <0.10 08/06/02 1502 08/05/02 1147 08/07/02 1446 Intake 08/04/02 1256 to 08/06/02 1040 0.5 <0.10 08/06/02 1502 08/05/02 1156 08/07/02 1446 101 08/06/02 1257 to 08/08/02 1125 1.21 <0.10 08/08/02 1500 08/07/02 1157 08/09/02 1509 Intake 08/06/02 1245 to 08/08/02 1125 0.5 <0.10 08/08/02 1500 08/07/02 1145 08/09/02 1509 101 08/08/02 1332 to 08/10/02 1051 1.71 <0.10 08/10/02 1456 08/09/02 1232 08/11/02 1443
, 08/12/02 1442 Intake 08/08/02 1303 to 08/10/02 1051 1.5 <0.10 08/10/02 1456 08/09/02 1203 08/11/02 1443 1 108/12/02 1442
- TRC = Total Residual Chlorine tCollected in two 2.5-gallon cubitainers. Temperature was measured in each cubitainer upon arrival
-4. Sample Manipulation: Samples from Outfall 10 1 and the Intake were warmed to test
.temperature (25.0 +/- 0 C in a warm water bath.
Aliquots of Outfall 101 and Intake samples were l/V-treated through a 40-watt Rainbow Lifeguardo UV Sterilizer for 2 minutes.
3
Pimephalespromelas Ceriodaphniadubia Test Organisms:
- 1. Source: Aquatic BioSystems, Inc. In-house Cultures
- 2. Age: 23.5-26.5 hours5.787037e-5 days <br />0.00139 hours <br />8.267196e-6 weeks <br />1.9025e-6 months <br /> old < 24-hours old Test Method Summary:
- 1. Test Conditions: Static, Renewal Static, Renewal
- 2. Test Duration: 7 days Until at least 60% of control females have 3 broods
- 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic
- 4. Number of Replicates: 4 10
- 5. Organisms per Replicate: 10 I
- 6) Test Initiation: (Date/Time)
Outfall 101 08/06/02-1502 EST 08/06/02-1214 EST UV Treated Outfall 101 08/06/02-1529 EST
- 7) Test Termination: (Date/Time)
Outfall 101 08/13/02-1422 EST 08/13/02-1146 EST UV Treated Outfall 101 08/13/02-1446 EST
- 8) Test Temperature: Outfall 101: Mean =24.8 °C Mean = 24.8 0C (24.3-25.60 C) (24.2-25.60 C)
Test Temperature: UV-Treated Outfall 101: Mean =24.9°C (24.3-25.5 0 C)
- 9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post exposure test solutions were analyzed daily for pH and dissolved oxygen.
- 10. Statistics: Statistics were performed according to methods prescribed by EPA using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).
4
TOXICITY TEST RESULTS (see Appendix B for Bench Sheets)
- 1. Results of a Pimephalespromelas Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 6-13. 2002 using effluent from Outfall 101.
Test Percent Surviving Solutions (time interval used - da s)
(% Effluent) 1 '2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.98% 100 100 100 100 100 98 98 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 95 93 72% 100 100 100 100 100 100 100 100% 100 100 100 100 100 100 100 Intake 100o 100 100 100 100 100 100 96-hour LC5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2 3 4 Mean Control 0.745 0.780 0.873 0.964 0.841 10.98% 0.533 0.609 0.667 0.766 0.644 22% 0.754 0.714 0.846 0.631 0.736 43.9% 0.683 0.618 0.697 0.577 0.644 72% 0.937 0.719 0.823 0.681 0.790 100% 0.753 .0.729 0.720 0.652 0.714 Intake 0.819 0.787 0.793 0.769 0.792 IC5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits: NA Permit Limit: 1.0 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC5 0: TUc = 100/IC25 5
TOXICITY TEST RESULTS (see Appendix B for Bench Sheets)
- 2. Results of a Ceriodaphniadubia Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 6-13, 2002 using effluent from Outfall 101.
Test (time interval used - days Solutions
(% Effluent)
IT 5 6 7 Control 100 0oo 2 100 100 100 100 100 10.98% 100 100 100 100 100 100 100 22% 100 100 100 100 100 100 100 43.9% 100 100 100 100 100 100 100 72% 100 100 100 100 100 100 100 100% 100 100 100 100 100 100 100 Intake 100 1 W') 1 M)I inn1 I ... .UU
... I.
96-hour LC5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA Reproduction (#young/female/7 days)
Test Solutions Data (re plicate number)
(% Effluent) 1 2 3 4 5 6 7 8 9 10 Mean Control 27 "25 28 32 28 24 30 32 28 '30 28.4 10.98% 27 36 34 32 33 31 31 29 31 31 31.5 22% 30 29 30 31 31 28 28 32 27 31 29.7 43.9% 34 26 31 30 33 33 33 32 32 30 31.4 72% 31 32 33 37 31 31 36 34 31 32 32.8 100% 32 31 34 35 31 37 38 35 36 33 34.2 Intake 28 28 25 30 25 27 26 26 30 31 27.6 IC 5 0 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUc Lower Limit: NA
- TUa = 100/LC 50 : TUc = 100/IC2 5 6
TOXICITY TEST RESULTS, UV-TREATED (see Appendix B for Bench Sheets)
- 3. Results of a Pimephalespromelas Chronic 7-day Toxicity Test.
(Genus species) (Type / Duration)
Conducted August 6-13, 2002 using effluent from UV Treated Outfall 101.
Test Percent Surviving Solutions ,time interval used - days)
(% Effluent) 1 2 3 4 5 6 7 Control 100 100 100 98 98 98 98 10.98% 100 100 100 100 100 100 100 22% 100 100 100 100 98 98 98 43.9% 100 100 100 100 100 100 100 72% 100 100 10( 1 100. 100 98 98 100% 100 100 10C 1 100 100 100 100 Intake 100 98 98 98 98 98 98 96-hour LC5 0 Value: > 100% Calculated TU Estimates: <:1.0 TUa*
95% Confidence Limits: NA Upper Limit: NA Permit Limit: 1.0 TUa Lower Limit: NA, Mean Dry Weight (mg)
Test Solutions (replicate number)
(% Effluent) 1 2 3 4 Mean -
Control I I 0.869 0.846 0.676 0.766 0.789 10.98% 0.897 0.704 0.649 0.853 0.776 22% 0.8850 0.684 0.899 0.795 0.816 43.9% 0.679 0.822 0.878 0.899 0.820 72% 0.581 0.730 0.740 0.690 100% 0.685 0.607 0.935 0.859 0.617 0.755 Intake 0.747 0.786 0.815 0.665 0.753 I 0.753 0.747 0.786 IC50 Value: > 100% Calculated TU Estimates: < 1.0 TUc*
95% Confidence Limits: NA Permit Limit: 1.0 TUc Upper Limit: NA Lower Limit: NA
- TUa = 100/LC50 : TUc = 100/ IC 25 7
REFERENCE TOXICANT TEST RESULTS (see Appendix A and C)
SUMMARY
/ CONCLUSIONS Outfall 101 samples collected August 4-9, 2002, showed no toxic effects to fathead minnows or daphnids. The resulting IC 25 values, for both species, were > 100 percent.
Exposure of fathead minnows and daphnids to intake samples resulted in no significant differences from controls during this study period.
Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah.
Although dose response and survival in the routine compliance test were not impacted enough to jeopardize test acceptability or compliance with permit limits during this study, survival was improved by two minute exposure of samples to UV light prior to introduction of test organisms.
These results suggest that fish pathogen interference is a likely cause of historic problems with WET tests for this effluent.
8
Appendix A ADDITIONAL TOXICITY TEST INFORMATION
SUMMARY
OF METHODS
- 1. Pimephalespromelas Tests were conducted according to EPA/600/4-91/002 (July 1994) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 400-mL polypropylene beakers, each containing 250-mL of test solution.
- 2. Ceriodaphniadubia Tests were conducted according to EPA/600/4-91/002 (July 1994) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.
DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS
- 1. Pimephalespromelas None
- 2. Ceriodaphniadubia None 9
PHYSICAL AND CHEMICAL METHODS
- 1. Regents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration dates (where applicable).
- 2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.
- 3. Temperature was measured using EPA Method 170.1.
- 5. The pH was measured EPA Method 150.1.
- 6. Conductance was measured EPA Method 120.1.
- 7. Alkalinity was measured using EPA Method 310.1.
- 8. Hardness was measured EPA Method 130.2.
QUALITY ASSURANCE Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA/600/4-91/002. Any known deviations were noted during the study and are reported herein.
REFERENCE TOXICANT TESTS (See Appendix C for control chart information)
- 1. Test Type: 7-day chronic tests with results expressed as IC values in g KC1 25 or NaCI.
Sodium Chloride (NaC1 crystalline) for Ceriodaphniadubia.
- 3. Dilution Water Used: Moderately hard synthetic water.
- 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.
10
REFERENCES
- 1. NPDES Permit No. TN0026450.
- 2. Lewis, P. A., D. J. Klemm, J. M. Lazorchak, T. J. Norberg-King, W. H. Peltier, M. A. Heber.
Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-91/002 (July 1994).
- 3. Methods for Chemical Analysis of Water and Wastes, EPA/600/4-79/020 (March 1983).
11
Sequoyah Nuclear Plant Biomonitoring September 6-13, 2002 Appendix B Diffuser Discharge Concentrations of Total Residual Chlorine, Diffuser Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, And Initial and Final' Chemistry for Fathead Minnow 7-day and Daphnid 3-brood Chronic Tests 12
Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 9, 2002 13
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria'and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 9, 2002 14
Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced Bacteria and Asiatic Clams, During Toxicity Test Sampling, March 12, 1998-August 9, 2002 15
PHYSICAL/CIEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for Pimephalespromelas and Ceriodaphniadubia Tests, Sequoyah Nuclear Plant Effluent (SQN), Outfall 101, August 6-13, 2002.
Test Sample ID Temperature ( C) Dissolved Oxygen (mg/L) pH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Final (pmhojlcm) (mg/L ClCO') (mg/L CaCo,) Chlorine (mg/L)
Pimephales Control 24 5 24 9 81 76 8 05 7.73 308 64 87 promelas 243 -24.7 245- 252 78 - 83 7.3 - 81 802- 807 761- 802 301 - 317 63 - 66 85 - 89 1098% 245 24.9 82 75 807 768 293 244 - 24.7 245 - 252 80 - 83 73 - 82 802 - 809 756 - 803 285 - 302 22% 24.524.6 249 82 74 806 768 282
- 251 246 - 253 80 - 83 68 - 82 801 - 810 7.55 - 806 274 - 291 439% 247 24.9 82 74 805 763 260 245 - 25.1 246 - 252 80 - 84 68 - 81 800 - 810 752 - 790 250 - 273 72% 250 249 82 73 804 764 231 24.5 - 253 244 - 252 80 - 84 67 -82 797 - 810 7.53 - 794 222 -237 100% 251 249 83 73 802 764 202 73 86 <010 245 -256 245 -252 8.1 - 84 67 - 82 794 - 810 752 - 801 193 - 211 69 - 78 83 - 87 <010 - <0.10 Intake 24.9 249 83 75 800 768 205 71 84 <0 10 245 -255 245 -25.1 82 - 84 7.1 - 83 787 - 809 7.57 - 802 192 - 215 69 - 73 79 - 89 <010 - <010 Certodaphnta Control 24 5 249 8.1 82 805 808 308 64 87 dubla 242 -24.7 245 -252 78 - 83 80 - 84 802 - 807 802 - 817 301 - 317 63 - 66 85 - 89 1098% 246 249 82 82 807 809 293 243 - 249 24.5 - 252 80 - 83 80 - 8.3 802 - 809 803 - 819 285 - 302 22% 246 249 82 82 806 808 282 244 -250 245 -252 80 - 8.3 80 - 82 801 - 810 802 - 818 274 - 291 248 248 82 82 805 810 260 4_.9_ 246 -253 244 -25.2 80 - 84 81 - 82 800 - 810 805 - 819 250 - 273 72% 249 248 82 82 804 809 231 246-25.5 24.5 -252 80 - 84 81 - 83 797 - 810 802 - 8.18 222 - 237 100% 250 248 8.3 . 83 802 8 11 202 71 84 246 -256 243 -25.2 81 - 84 81 - 83 7.94 - 810 800 - 821 193
< 0.10
- 211 69 - 73 79 - 89 <010 - <010 Intake 124.4249
-252 24.7 8.3 82 800 807 205 71 84 < 0.10 243 -252 82 - 84 81 - 84 787 - 809 799 - 815 192 - 215 69 - 73 79 - 89 <0.10 - <0.10 16
PHYSICAL/CHEMICAL
SUMMARY
Water Chemistry Mean Values and Ranges for the Pirnephalespromelas Test, Sequoyah Nuclear Plant Effluent (SQN), UV Treated Outfall 101, August 6-13, 2002.
Test Sample ID Temperature (OC) Dissolved Ox ygen (mg/L) PH (S.U.) Conductance Alkalinity Hardness Total Residual Initial Final Initial Final Initial Pimephales Control Final (Vrmhos/cm) (mg/LCaCO,) (mg/LCaCO,) Chlorine (mg/L) 24.5 24.9 8.1 7.5 8.05 promelas 7.72 301 64 87 24.3 - 24.7 24.4 - 25.3 7.9 - 8.2 7.1 - 8.3 7.98 - 8.08 7.62 - 8.03 296 - 311 63 - 66 85 - 89 10.98% 24.7 24.9 8.1 7.5 8.04 7.73 293 24.4 - 24.9 24.4 - 25.4 7.9 - 8.2 7.1 - 8.3 7.98 - 8.08 7.59 - 8.02 284 - 306 22% 24.8 24.9 8.1 7.5 8.04 7.70 280 246 - 25.1 24.4 - 25.2 7.9 - 8.3 7.2 - 8.3 7.98 - 8.08 7.60 - 7.99 272 - 294 43.9% 24.9 24.8 8.1 7.4 8 04 7.68 . 258 24.7 - 25.3 24.4 - 25.2 7.9 - 8.3 6.8 - 8.3 7.98 - 8.08 7.54 - 8.02 245 - 269 72% 25.1 24.8 8.1 7.4 8.03 7.69 229 24.7 - 25.4 24.4 - 25.2 7.9 - 8.2 6.7 - 8.3 7.97 - 8.08 7.56 - 8.04 220 - 238 100% 25.1 24.8 8.1 7.5 802 24.7 - 25.4 24.4 - 25.2 7.8 - 8.3 7.73 201 73 86 < 0.10 7.2 - 8.4 7.96 - 8.09 7.58 - 8.02 193 - 209 69 - 78 83 - 87 <0.10 - <0.10 Intake 25.0 24 8 8.2 7.5 7.97 24.6 - 25.5 24.4 - 25.1 7.70 199 71 84 <0.10 7.9 - 8.3 7.2 - 8.3 7.75 - 8.07 7.56 - 8.04 190 - 212 69 - 73 79 - 89 <0.10 - <0.10 17
Sequoyah Nuclear Plant Biomonitoring September 6-13, 2002 Appendix C Chain of Custody Records and Toxicity Test Bench Sheets
BIOMONITORING CHAIN OF CUSTODY RECORD Client: TVA Environmental Testing Solutions, LLC Project Nane" TVA SQN Research Building Bus Client P 0. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Facility Sampled: SEQUOYAH NUCLEAR PLANT I Phone: 828-862-8193 General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN
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yet Instructions: Clientsfirom should sumple is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> the fill timeinofallLollection.
areas except those in the "Laboratory Use" block. Biomnonitoring samples'are preserved by storing them at 4C and shipping them in ice. The hold time for each Therefbre, please collect and ship in such a way that the laboratory will receive the Samples shipped overnight on Friday via FedEx or UPS must be marked fur Saturday samples with ample time to initiate testing within that time frame.
delivery or they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page I of Client: TVA 1 Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):
Facility Sampled SEQUOYAH NUCLEAR PLANT Phone: 828-862-8193Comments:
NPDES Number: 0026450 Fax: 828-862-8!95 Collected By: WANDA ALLEN Container F ield Id en silic a t ion r b eSp C ollectio n D ate /lbnhe N um b er & Flo w R a in E ve n t? . . . . * .... .. :
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they will not arrive until the following Monday.
BIOMONITORING CHAIN OF CUSTODY RECORD Page 1 of I Client: TVA Environmental Testing Solutions, LLC Delivered By (Circle One):
Project Name: TVA SQN Research Building FedEx UPS Bus Client P.O. Number: PO BOX 2000 1 Ecusta Road, Brevard, NC 28712 Other (specify):_
Facility Sampled: SEQUOYAH NUCLEAR PLANT Phone:General Comments:
NPDES Number: 0026450 Fax: 828-862-8195 Collected By: WANDA ALLEN C ontainer
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Date/Time Instructions, Clients should till in all areas except those in the "Laboratory Use" block. Biomonitoring sample is 36 hours4.166667e-4 days <br />0.01 hours <br />5.952381e-5 weeks <br />1.3698e-5 months <br /> from the time of collection. Therelore, samples are preserved by storing them at 4@C and shipping please collect and ship in such a way that the laboratory them in ice. The hold time for each Samples shipped overnight on Friday via FedEx or will receive the samples with ample time to initiate UPS must be marked for Saturday delivery or they will testing within that time frame.
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ironmental Testin, Snlntinns_ T.T.r
, " Page I ot s Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas Client: "IO'.
Facility: mo-,1 cie..t Pal*&*
. apw NPDES#: 7142LA*p i nreparationinformation: Comments:
MHS batch: 61-'z 2.0 ba'*- /ca-. -o7. o e Diluton r*p(%) io.'067zA 1.i 1. O Eflluat volume (mL) 100 Dzducn =o(mneL) .3 ~ 1jJ Test organisminformation: Test information:
Organism age: - -.- A&JOiS ntlý 23.7 -39S-J* Randomizing template: LLOG)
Date and times organisms t-o o ..- 10!&o o Incubator number:
were born between: -1.2. ij &Z-*'0 H - Z Organism source: Gf-0-d2.
oM Artemia lot number:
Transfer bowl information: pH = Temperature = Oven temperature: C.
-_.__91 2-_q &.C. Drying time: IqL*,s Dailyfeeding andrenewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 Ot,0-oz0 - - 1 ISO 7 2
1.oA -o107 ..-. 1 05 16 S.
32____ OE.
O oMt --007.
7- 10-!_.
1052. 16c4 05q 16c-60 "
4 0- 1ozi- 1000 hooo Controlinformation: Aeeptanc critea Summary of test endpoints:
%Mortality: :520% 7-day LC50 >_Io 01.
Average weight per larvae: 0. >
ŽOL025 mS arvae NOEC /0tO7.
LOEC ChV .> IXVr IC25 I > too,
,,.nnma.n+al uuu.at.a.a. T..tna . n1utinn J---.----, TIC Page 2 o b ental Ir"+;n S-111flow LLC Species: Pimephalespromelas Client: !ein flAdAl-r.e*,*b
- Date: o&0 6--*- I Survival and Growth Data Day CONTROL -0. lo. & -
A B C D E F G H II J K L 0 /0/0/0 /0 /0 1o /0/0 /0 /0 /0 /0 1 /00 i/01 1 0/0/6/0 /0 /0 2 /Ic /0 /A /0 /0 /0 /A /0 /0 /0 /D /0 3 Jo Io 16 0 /obi Io)O ID~1i0 1O( OR 1(
4 I0 i/ 1 /0 1 0 (to 0 !0 /0 /0 o0 /6O to 7O i0 10 to 1 IO o /o /0 10 1/6 A_ - -a ...
B~- (mg + - -a -~-a -egh -D 9
B-Pn+Larvae weight (mg) B g
-A A; -6~ { i P
Calculations and data reviewed:
Comments:
onmental Testing Solutions, LLC ,.b Species: Pimepha/espromelas Client: P,/J&Un_*
A eATE Date: O-rie-l-2.
Survival and Growth Data Day - 07a -7zW /W M
MIN_0_1 N P IR S T U V W X 0 /01o /
1/0 10o 'i'v /0/0 /0 /o0 /O /0 1 0 Ilo /0 /0 m L) 10 1(0 /()
I0 /0 /0
)
2 }O /0 ( I0 /0 /0 /0 /0 10 10 /0 /0 /0 3 / /0 /0c /0 /0 10 10 t0 10 /0 /0 (0 4 /0 10 /O/0 0 (O /1 1 /0 /0 1/0 /0 5 /0 101t 1 /0 1 /00 / 1 /0 1D D /It I0 6 / 1, (I0 L) fo lo Ioto 1o Lo) 1I/0 /0 7 q *b) L) 8 /01 O 1010U -I -o -o J(>~~~C A
01 1 1 11/0 B~~~~~ a 5 avewegt(g -
1 '( 1 0 B...,*-,=,,e~<*.* ID"*'*' ~ *
- I.X.*'@'I'* '*P ,*"*
9.211 4. 1WP
" *P"*'"*/"0 C.2 BaLarvae weighIt(mg) A B~ - -' 14 " .1 1 1 1 D
Calculations and data reviewed.
i.-vu4"&CuUU 1I3LIUX, allUtonIs. LLC Page Pg 4 ot1 8 Species: Pimephalespromeias Client: SPI~sAA1-' NQEE~n Date: 6,9O0o' 07-Survival and Growth Data DayI* T
__ _I_ Z AA BB 1 10 10
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&0 B -Pan +Larvaeweight (mg) lfbb a'L zt-v Larvae weight (mg)-A - B 1.1V V0 I Calculations and data reviewel, ,A9 Comments.
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dales: May 7.14, 2002 Revdwed br.
Project number: 324 mbfof Filnal ambeolatlarvate A-= tawtght B - Pa LarvIe Larvae *wed l- (n Welth00I latal a-a ah' a l M-a- wdb
, .. g) Cu
._ .1 t s uid LCoatntntitoa(%) Rheplical¢ lv.Walt
('-1) Clubs(211) -A- B Oftv*aam.53 (%r*)wit on (%l claw (%)
ta q 10 to 14 637 "22090 7 453 0.7453 A
07798 1000 0 8406 " 11.7 Not appucable B
C 10 10 10 to 15012 14 660 22810 23 390' 87.798 730 08730 onrl 24 560 9 643 . 0 9643 !.
"D 't0 10 *"14 917 5 334 0 5334 SE to 9 14 916 20 250 6086 06086 97.5 0,6439 85.3 23A 0F 30 10 14604 20690 G 10 10 14687 21360 6673 06673 15078 22740 7662 07662 I =
ii 10 10 S 1010 14 999 22540 7541 07541 07142 1000 0.7361 12.1 12A 10 F0 15008 22150 7142
%J 10 3K 14915 23370 8455 08455 t
10 3 10 14573 20880 6307 06307 .. _,,,
M 10 9 14948 21.780 6832 06832 6182 06182 92.5 0.6440 8.7 23.4 N 10 10 14648 20830 010 015057 22030 6973 06973 20790 5774 05774 . ,
p 10 8 15016 100 14988 24360 9372 09372 21760 7185 07185 3000 0.7898 146 6.0 R to 310 14575 S10 314801 23 030 8229 08229 21510 6805 06805 ,, . .. ..
T 1 030 14705 V 10 10 14713 22240 7527 07527 7293 07293 1000 0.7135 6.1 15.1 10 300 10 14567 21860 10 10 15030 22.230 7200 0 7200 100% ,
X 10 10 14799 21320 6521 06521 .,.
to 10 14916 23 330 8 194 08194 7867 07867 3000 0.7921 2.6 5.8 100%10 34 963 22830 10 15067 23000 7933 07933 100%AanAke ..
BB 10 130 14631 1 22320 7689 07689 MSD - Minimum Significant Difference Outfam101 PMSD - Percent Minimum Significant Difference 0.1486 that can be Dunnstt's MSD value:
17.7 PMSD is a measure of test precision. The PMSD is the minimuIn percent difference between the conirOl and treatment PMSD: Envlronmental declared statistically significant in a whole effluent toxicity test On average, a significant difference oeeui for (determined Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 20.2% from the control Intake, Lower PMSD bound determined by USEPA (10th percentile) - 9 4%.
Dunnelt's MSD value: 00979 336 Upper PMSD bound determined by USEPA (90th percentile) - 35%.
PMSD:
"Tlie lower and upper bounds were~calculated by the USEPA using 205 tests conducted from 19 laboratories for Pimelihalcit growth in chronic reference toxicant tests the National Pollutant Discharge Elimination Program. EPA-833-R-(0)-003. US Envirownental USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under Protection Agency, Cincinnati. 031
Environmental Testing Solutions, LLC Statistical Analyses Larval Iru Grow* MdSaiv~d Ttal. DayGmw4=
StartDO &6 ooM rp Stople 0 TVA SepwYib Nwu ~w -dOma&0101 Bad Do (13M0 LAbM. 3T30.. Tetn Soo 2891.2)y Sowk4 o P.aa ZPAF 9147A fToalwara TONSpeco MPPa~
-"putd Commence:2 S 04:0M.0 0.7453 0.779 0.AM3 0-%Q4 Ia."1 O.S34 0.606 0.673 0.7662 22 0.7541 4.7142 0.35 0.A307 43.9 O=63 U.132 0ohm7 0.3774 72 0."372 0.7191 0.632 0.600 too 0.752 0.729 0.7200 0.6521
-- -TacatwmlVUUdawfmd 1-Tullad tal Cto%Mt NMt Ma MIN Mas CV% N I'Stt Critical '.15 Meat 14Mat 0.czecro 0 &406 1 D 0.11406 0,7453 0.9643 11723 4 0.3406 1 0007
-10.98 0.6439 0.7660 0.6439 0.5334 0.7662 13.260 4 3 190 2.410 M1496 0.7051 0.6392 22 0.7361 0.657 .736 0.6A307 0645 12.120 4 1694 2.410 0.1496 0.7055 0.1392
-439 0.6MG 0.7661 0.6w4 0.5774 0.6M7 L.728 4 3 108 2.410 0.1496 0.7055 00392 72 0.7396 09395 0 739 0.6W0 - M.372 14.594 4 0.324 2.410 0 1466 0.7055 0.192 too 0.7135 0.11468 07135 0.6521 07527 6&054 4 2.061 2.410 0.1466 0.7055 0.8392 Auxiliary Test& Stathoc CrItcaL 66*. Kan Sbw.ýVWiks Tea ad-c= normaldL~btbd* (P > 0 01) 0.9451765.42 066 a" 20352782 46179585716 Badeos Tea caleaa o;,W a(p - 0673 319022942S I50"631706 11potheck Tes It-tai. CL081 NOEC 1.0CC City TU Anu1. NZDP MUB 9681 T.Prob d1 Duance1sTea too -l00 1 0.14W67397 0.176199763 0024719414 000760561 0020946454 5.111 LineaurInterp-lton(80 Elm Pleuaa)
Point % SD 95%CL(Exp) 66*."
pm.5 34t3 I0190 3.513 U5497 11.6024 ICLS* 10.245 1.0.
1C40 '107 4 08 IMS >100 07.
uodtaa IC aesrate taxthCan thelowestcoaaetramno 0.5.
- 04 0.3 01 0 20 40 60 80 107 120 Doa.SlaoDose%
1.2.
1-tod. 005I level sigrunce litu 04 0.2 I
9! 8 4,
.rqnO&8-06-02dat-r&
Environmental Testing Solutions, LLC Statistical Analyses T
'I L4arvYl Fb Gcowe &adSarntvl Tat-7 Day Grow't Soni Datm 8V6402 Test ID PpFPCR Sample D TVA Sequoyah Nues Plant. [-take End Daw 8/13102 Lab W, ET-Env Testng Solutons SampleType NoUaedated Sample Dow Protocol EPAF 91-EPA Freshwa Test Specia PP-Purephales promels Comeiats Came-'/ 1 2 1 A D-Contron 07453 07798 08730 09643 100 0.3194 07867 07933 07689 Tmansform- Untrinsformed I-Tadtd Coatc-% Mean N-Meas Mea MstM Max CV% N t-Stat Critical MSD D-Cormol 08406 10000 08406 07453, 09643 11723 4 100 07921 09423 07921 07689 0.3194 2642 4 0963 1943 00979 Auxiliary Tests Statstic Cnticil Skew Kurt Shaptro-Wk's Test midciates nomal dusmbuton (p >0 01) 0 962719679 0749 0 560729505 1 1222114493 F-Test aidicates equal variances (p - 0 03) 22 16693497 4746834564 Hypothesis Test ([-tad.0.05)
Honoscedastc t Test indicates no silpnficant differtnces sqnO8-06-O2data.xLr
Chronic Whole Effluent Toxicity Test (EPA/600/4-91/00 2 Method 1002.0)
Species: Ceriodaphniadubia Date Client: "T'*/A Facilty:.
NPDES #:
Calculations andatarviwd Test Renewal, Feeding. and Incubator Locao Informaton Mon Tue Wed Thu Date ctL:-r- Fri Sat Sun oj!(- 0--ol ýý U Time g 0I+/- Oe.'II I!1 0" Anhlt 44 azz Location
~ _
a&UUAa-J 5* i estlng bolutions-, LLC Page 2 ot s Species: Ceriodaphniadubia Client: -r Ae~rIA Dte f J9A '7 Dat:
CONC ION NTRA ~Survival *1 2 andRep oducio Data 2 Younh produced 10 eI I 1. )J Youngrinproduced I
%Moraliy:
Mea C1-5%
7 5 Yeoutio produCntrod -io 9 Calculations and data reviewed:
CONCENTRATION: 22%
4
Species: Ceriodaphniadubia Client: *,P :4AA Date: -l~o'o-.
CONCENTRATION: _q 1*P,,,
Day 1 2 3 4 1 5 6 7 8 9 10 1 Young Produced' o ... ... o...,.
Adui-m*ort y .. L . L_
2 Young produced p p p p . , C" Adult mortality _ U - L U.-.
3 Young produced x C U i AA d ult mortality Ut? - U .. T...L--.
4 Young producedo AduMtmortaltyLt 5 Yuan produced ,e ,
Adult mortality I_ ._ z T U 10 6 OCENTAtTION Young produced L. L u 1 a a --
U D_ _ 1L.- L_
7 I Young prod uced I youg 31 9 roucd ZfJ 1 o 33
(-
C' 33 f 1 33frm 32.
1 14R 37.- -30 F Adult A Mortality d -euto otrl ,*+
L.1_ -1 -= . L_
Concentration:
% %Mortality:CZ Mean Offsprng/Female: 31 "A
%Reduction from Control: - 1, 1 Calculations and data reviewed:
CONCENTRATION: .Survival andReproduction Data
- ____ ~Replicate number _ _
Day___ 1 2 13 4 516 7 18 9 11io I Young produced a -0 - - -n Te-Adultmortalit L L 21 Young produced [ C') 0 0IQ0_11 L __L_
I D1 L-0 t_
Adult mortality U .
3 1 Young produced 0. 1" 0 6____i I LL_ IL_ -_Admi L_
6 Yotmg roduced ~ I T T Adult mortality L JL- U 7 Youmg produced /7 / 0 0 I 1'7
-0 1 Total young produced 1 3 3 L3- 3 1.
- 3 1 .3 Final Adult Mortalitv L_ U.I C~ U j L. L U. '-
Concentration:
I%Mortalily: I cz I Mean Offspring/Female: 132. &
% Reduction from Control: =- IS X.
Calculations and data reviewed:
Species: Ceriodaphniadubia Client:RATION:
EIate. -
CONCENTRATION: cI 13urvwal and "rodadion Data namber Cncet 3 Young produced o10 9 Adult mortality 2 Youg produced Adui mortality 0 0 G n 4 Young produced Adult mortality 6 Young produced
- m Adult ortzality-* 14_
6 Young prodcedtrattion Adl Mortalityy 0 C 7%on Redudtion fro Cotrl -2q*
Final -o ;AI*I FTATd ONt '. Suv ladtlcltosaddaarve e: _
Concentation
[~~~ ~
Mortalritiyy:{*(*
t.-i %.t.ot o.-f. m CoG . ..- T--- f.
I Young produced Adult mortality
.. l,.
2~t. Yon prdue ** L 4 Young produced I( ~ *I* ^ L. - .,* ,
"j j Adult mortality IL- Lic iL~ 'L 7 Young produced Total young produced in f
I P1 I '-s l'-4 I Iq~ 11-fCc
~ I i77f
.21 -
~
a.. F7T -_
Final Adult Mortality I 2G(, 3O3I 1 I-
- - ~.- - h- I Concentration:
%Mortality: I ,
Mean Offspring/Female:
% Reduction from Control: Z.97 Calculations and data reviewed:
_1k
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4.91/ 0 02 , Method 1002.0)
Species: Ceriodaphnia dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, Nontreated Test dates: August 6-13, 2002 Project number: 405 Minimum Significant Difference 2.372 Percent Minimm 8.4 PMSD is a measureSignifiLant Difference Of test precision.
significant in a whole effluent toxicity The PMSD is the minimum percent difference between the contro and test. On average, a significant difference trent t b e when a toxicant reduces Ceriodaphnia occurs for Envimnmental Testing inett's MSD value: reproduction by 11.8% from the control, Solutions, LLC chronic toxicity tests 1.889 3D.- Lower PMSD bound determined by 6.7 USEPA (I 0 tdpercentile) = 1I%.
Upper PMSD bound detennined by USEPA (90'h percentile) - 37%.
The lower and upper bounds were calculated by the USEPA using 393 reference toxicant tests. tests conducted from 33 laboratories for Ceriodaphnia reproduction in chronic
......Agency, Vtion .- n s,-nimng and Accounting for Method Variability Cincinnati, Oil. in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination
Environmental Testing Solutions, LLC Statistical Analyses Calodaphak Suryaladu W op cdogTest-Repydaedon Stan Dim W602 Tst VD CFLRCK SemIpea) TVA Seaqoyda Nudw ram%04 1101 End Dae* S13/02 Lab ID ErS-Euv Taen So.lumon Sampie Type DMl-Dutcba, Moi 'ngReo*t SempleDamP rrocol: EAF 9 I-EPA Rahwate Tes SpeC: CDCumdaplina clubia Commem C.om.% 1 2 3 4 5 6 7 3 9 1*
D.CoUrI 27000 25000 2110001 32.000 28000 24000 30.0 32.000 23.000 30.000 10.98 27.000 36000 34.000 32.000 33.000 31.000 31.000 29000 31.000 31.00 22 30000 29.000 30000 31.000 31.00 28.00 28000 32M000 27.000 31.000 43.9 34000 26 00o 31.000 30 00 33000 33.00 33.000 32.000 32.0M0 30000 "72 21.000 32.000 33000 37000 31.0 31,000 36000 34000 31.000 32.000 10o 32.000 31.000 3,4000 35. 31.00 37.000 32.0 3S000 36000 33.000 Tranfomr: Unwansformed I-Talled I"Wakc C6n6-1/ Mean N-Me"a MeaN Mi Mal CV% N t-St CrItICAL MSD Mean N-Mean D.-Conml 28400 1000 28400 24000 32000 9419 10 31.333 1.000 1091 31.500 11092 31.500 27000 36000 7954 10 -2988 2.287 2.372 31333 10000 22 29700 10458 29700 27000 32.000 5.510 10 -1253 2287 2.372 31t333 .000 439 31400 11056 31400 26000 34 000 7385 10 -2.892 2.287 2.372 31.333 1 M00 72 32.800 1.1549 32.800 31000, - 37000 6710 10 -4141 2.2M7 2.372 31333 L0000 100 34.200 1.2042 34.200 31000 38 000 7136 10 -o.59I 2.2n7 2.372 31.333 10000 Auxtmry Tes Stadsc Crical -Skew Kurt Kouiogorov D Test mndicar normal dainbuton (p > 0 0 1) 0.561091602 1035 -0 12299514 -0 24073858 Bardletes Test ndicates equal vanances (p 0 821) 2272905827 1508631706 Hypothests Test (1-t*I* 0 05) NOEC LOEC ChV TU MSDu MSDp M.SB MSE F-Prob 4f Duane 'sTesc 100 >t00 t 2372295457 0.08353t53 4334666667 5.381481481 99E-06 5,54 Linear IntespoLation (90 Resamples)
Point % SD 95% CL Skew ICO5 > 100 (CI0 >100 ICis >200 1.0 I*'O >100 09:
IC25 >100 08 1C40 >100 07 1C50 >100 0
- 05.
r 04.
- -03 0.2 0
01 000
.0.1 ... .........
-0 3 . . . . . . . . . . . . . . . . . .
-02 0 20 40 60 80 100 120 Dose %
DoswResponse Plot At*l 35 o25 30
...................... ................. 1-tail. 0 05 level of significance 20 0.
- is 10.
5.
in ci 8 sqnO8-06-O2data.xLs
Environmental Testing Solutions, LLC Statistical Analyses Crniodhphnia Survtval and Reproductlom Test-Reproduction Stni Daze- N6z0Z Test ID CdFRCR Sam*pte ID TVA Sequoyah Nucear Pnt=*n Itake End Dale St3d02 Lab ID ETS-Env Tesmar Solzoons Sample Type DMR.-Dwchare MooWnntng Repnot Sample Dame Protocol EPAF 91 -EPA Freshwat" Test Species CD-Canodalphna chibua Comements Cone-% 1 2 3 4 5 6 7 $ 9 t0 D-Control 27000 25000 28000 32000 28 000 24000 30000 32000 "28000 30000 1o0 23000 23000 25000 30000 23000 27000 26000 26000 30000 31000 Tirsntorm Un-asiformed -Taided Conc-% Mean N-Meam Mean Min MMax CV% N t-Stat CrtIal NISD D-Contrl 23400 10000 28400 24000 32000 9419 10 100 27600 0971s 27600 25000 31000 7 a64 t0 0734 1734 1589 Auxiliary Tests Stanstic CnOtiCl Skew Kurt Shaptiro-Wilk's Tet indicates normal disnibunon (p > 0 01) 0960157156 0 868 .0010531462 -0 873208303 F-Test indicates equal varinces (p - 0 54) I 51586797 634108572 Hypothesis Test (1-tail. 0 05)
Homoscediac t Test indicates no stgnificaznt differences sqn_08-O6-O2data.xLS
4 Page I ot s Chronic Whole Effluent Toxic ity Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas client: 77qA Facility: Se* ý IA-r *&c NPDES #: "rN nn-*ze*qt 0_- - 013 mb Dailyfeeding and renewal information:
Da Dte Morning feeding -Afternoon feeding Test initiation, renewal, Analyst time time or termination time 02- . m I *oto o2.
-ý
~ OI~r "
42
- 1 172 -- Zs I(O O 0 3 4 *0 . -' q *"'
Controlinformation: Acceptance cnrteria Sumnma o test endpoints:
%Mortality: 20% 7-day LC50 )g Average weight prlarvae: . 7'7..0 7 0.25 ua NOEC LOEC >100%
Chv >loop/,
IC25 1C25 - >/av-l
Lavirunmentaj Testing Solutinnq~ .TI 9 Page 2 018~
Species: Pimephlpoea Client: Setucr4Ael OV- TKME~rb Date: apOG )c-I-1 Dav I i'n~mrrnA~~JU Mva and Grown Data A
A in in 4Kia - ______-_a
__ __ __ _ __ ' I~ 1 I I i I I -T IV~I'.
0 I a ' J - - I L
10 I0 Ic0 /0 /6 10 10 A) /0 10 2 to I0 0:
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weight Pa
/0 - /~
A Pan +Lra weight (mg)
Larvae weight (xng) - A -B13
.4j - 'L w sý s~ V'~~~ftiC Calculations and data reviewed A Comments:
. "r.Luui i cutug aoiutions, LLC Page 3 at 8 Species: Pimephalespromelas Client: 45n( k- 0-eT Date: QR-06-0 -c2-
_______ _______Survival and Growth Data _ _ _ _ _ _ _ _
Day - -72 10017 N
-m__7N_5_P 0 R S T U V W X 0 /00006 0000/0 /6 1 p~10/0/00 6/0 6/6 c) 0 2 ~ e
/o ) 1 010 /0 /0/
236 1( to~I 10/)0 1-0(10/1010/ 10 1
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IV/) Vc lo /0 1o A -Pan weight (ing) X10 4-141D je~t t~ ?, vs~~
as\'
BPan +Larvae weight (mg) PO Rp7 Larvac weight (ing) - A - B 9~ *i ~6 i1~ ~2 .. I Calculations and data reviewed.
Comments:
Environmental Testing Solutions, LLC Page 4 o18 Species: Pimephalespromelas Client: 6MOrf* (]1-W e Date: 36*'02'Z.
Survival and Growth Data Day (CO% INTre Y Z AA BB 0 1/0 /0 /0 /o
__ __ _ __ __ _ £0 ____ iv ,/
to ' / /0 3
/
5
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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pirnephalespromelas Quality Contiol Verification of Data Entry, Calculations, and Statistical Analyses Client: TVA Sequoyah Nuclear Plant, UV Treated Test dates: August 6-13, 2002 Revdwcd by: t If(
Project number: 405 Coa~~nija weinht itR-hatt Larva Larvewimgh(s) /Nb+rvae a
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X 10 10 y 10 3o 1475G 22900 8150 08150 6646 0 6646 97.5 0.7532 I.7 46 Z 10 9 14634 21.280 100% Intake AA 10 10 14706 22.180 7474 07474 30 10 14972 22830 7858 07858 BB MSD - Minimum Significant Difference Outfall lo1, PMSD Percent Minimum Significant Difference aunnett's hSD value: 0.1900 between the contsol snd ft hmnt tiat can be 24.1 PMSD is a measure of test precision. The PMSD is the minimum percent difference PTMSD: occus, forEnvironmental Testing declared statistically significant in a whole effluent toxicity test On average, a significant differece LLC chronic toxicity tests when a toxicant reduces Pimephales growth by 20 2% from the control (deternmined through Solutions, 0.106 Lower PMSD bound determined by USEPA (10th percentile) - 9 40/.
Dunnetta' MSD value: Upper PMSD bound determined by USEPA (90th percentile) - 35%.
PMSD: 134 for Pimephales growth in The lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratoriea chronic reference toxicant tests Protection Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental USEPA. 2000 Understanding and Accounting for Method Vanability inWhole Effluent'roxictty Agency. Cincinnati, 013
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Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/60014-91/002, Methods 1000.0 and 1002.0)
Species: Ceriodaphniadubia and Pimephalespromelas Summary of Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: August 06-13,2002 Project number: 405 Reviewed by:
Pimephalespromelas Ceriodaphniadubia Overall Temperature (C)
(including all concentrations for initial and final temperatures) 243 25.6 248 0.3 24.2 25.6 248 03
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/O02, Method 1000.0)
Species: Pinephalespromelas Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: August 06-13, 2002 Project number: 405 Reviewed by: CL Concentration JParameter Da 0Fia Day Day2 D3 Initial Fnl Day4 Da5S Da Initial Final Initial Final Initial I I Fnl Initial Final -Inittid'al Final PH1 (SU) 8.07 802 805 767 803 Initial Fia 7.69 807 7 86 7.68 DO (mg/L) 78 81 80 4 805 7.68 802 7.8 76 82. . 333 Conductivity (limbos/cm) 317303 3 301 Control Alkalinity (mg/I. CaCO3 ) 66 63 63 Hardness (mg/I. CaCQ,) 87 85 89 Temperature CC) 24.4 25.2 245 2 44 1 24.5 245 24.7 25.1 243 pit (SU) 809 803 807 248 245 7.56 807 766 8.06 760 DO (mg/I.) 80 82 82 806 7.72 802 10.98% 74 80 74 83 7.6 Conductivity (Itmhos/cm) 299 8.1 7.3 8.-2T 7.5 8.3 288 302 289 7.
285 292 299 Temperature CC) 24 252 247 250 247 245 24.5 246 25.1 1
PH1 (SU) 8.10 806 246 24.8 245 25.1 807 - 760 810 755 806 7.66 804 7.59 806 7.67 DO (mg/L) 8.11 8.21 821 801 7.64 22% -- :287 278 741 80 7.11 8.2 75 Conductivity -(pimhos/cm) 82 6.8 82 7..5374 83 74 2 871 2 .3 2 . 278 2911 2776
-T. .... L
... .... q erature t*C1 274 280 286 ý I Tcm 246 245 25.1 246 24.7 PH (SU) 8.10 790 806 7 245 250 8.09 7.52 807 762 802 IDO (mg/L) 8.1 81 7.57 803 7.61 800 7.601 43.9% 8 7.3 80 7.1 82 7.4 3 68 82 4 Conductivity jmo/m 27 253 264 84 7.6 258 250 260 245 25.2 25.1 265 250 248 247 248 24.6 246 251 24.8 247 246 PHI(SU) 8.10 7.94 8 06 7 250*
09 7.55 806 761 7.97 82 40 69 7.57 7.99 7.65 7.98 753 72% DO(mIL 82 7.
Conductivi imhos/cm) 237 237 230 2-224 Temperature CC) 24.71 25.2 230 25.3 25 0 25 0 27 24.91 244 2451 25.11 25.1 249 PH1 ýat 8.10 801 806 7.56 8.07 25.2 25.1 806 7.66 7.94 7.57 7.94 DO (rng/L) 81 8.2 8.3 7.4 7.61 7.97 7.58 8 1 8.3 7.3 8.3 Conductivity(limhos/cm) 211 6.7 8.3 7 8.4]7 202 209 204 100% Alkalinity (rnglL CaCO,) 193 96 201 78 71 Hardness(mg/I. CaCO.) 69 87 87 TotalResidualChlorine(rng/Ll' <0 10 83 1U. 10 Temperature C.1 I
I CC) 2471 25.21 25 5 25.0 54 24 24.9 24.5245 5.
I 809 82 2.3 48 256 51 04 76 807 76 80 82 76 7.7 79 790 .6 82 795 77 20 9 2 032 2 02 1 21 5 100% Intake Alkalinity (mg/L CaCO4 ) 73 7 Hardness (mg/L CaCOC) 83 Total Residual Chlorine (mg.__ <0.10 <0.10 <0 0 Temperature CC) 2451 25.1 247 25.0 25 0 I I 4 45 45 2 25.11 25.51 24.81 2501 25.1
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphniadubia Daily Chemical Analyses Client: Sequoyah Nuclear Plant Non-Treated Test dates: August 06-13, 2002 Project number: 405 Reviewed by.-: l-Concentration Parameter Day 0 Da 1 Day 2
____ Initial I Final Initial Finl Initial IFinal Day 3 1 Da 4 Day 5 1 D2 6 Initial Final Initial Final Initial Final Initial Finl PH (SU) 8071 806 805 809 803 802 807 8.17 8.06 817 805 8051 802 803 DO (mg/L) 78 83 80 8.2 78 8 821 82 81 8.2 8.2 83 83 80 Cnrl Conductivity (1imhos/cm) 317ý ý 303 317 301 303 302 Control Alkalinity (mg/L CaCQ) 66 63I63 313 Hardness (mg/L CaCQ) 8785 89 Temperature (C) 248 244 24 25.1 24.7 24.9 24.7 252 242 245 246 248 PH (SU) 809 806 807 80 804 807_ 8.19 806 8.18 806 803 802 807 10.98% DO (mg/L) (pmhos/cm)
Conductivity 80 82 288 82810 83832 8.1 299 302 289 285 8.2 8.2 8.3 3 80 Temperature (C) r292 299 246 24 8 24 3 25 249 24.7 244 24.5 246 249 8.10 804 807 808 8.10 806 804 22% 816 806 802 801 802 DO(mgIL) 8.1 881 80 82 82 82 82 82 83 80 Conductivity (amhos/cm) 287 278 291 27 274 280 286 Temperature CC) 2471 24.8 246 244 2250 24.9 246 252 244 245 248 24.9 pH(SU) 8101 807 806 808 809 806 807 819 802 8.18 803 805 800 806 43%
DO(mg/L) 8.1 8.2 83 80 82 82 82 83 82 82 82 84 82 Conductivity (limhos/cm) 273 253 264 258 25-- 60 265 Temperature CC) 247 24.8 24.7 246 247 25 1 25.3 248 246 252 247 24.4 248 247 ni (SU) 810 8 12 806 808 809 805 806 7.97 8.141 7.99 802 7.98 805 72% DO (mgL) 81 8.3 82 81 80 8. 2 82 8.3 82 82 82 84 Conductivity (imhos/cm) 821 237 234 237 230 222 224 230 Temperature (C) 24.9 24 6 24 8 255 248 25 248 24.5 25.1 pH(SU) 810 12 8.06 8.10 807 806 8.20 7.94 800 7.97 DO (giWL) 8.1 83831 8.1 Conductivity (ttmhos/cm) 8. 831 831 8 . 8.3 8.3 848.
211 202 209 204 201 100% Alkalinity (mg/L CaCq) 78 69 lHardness (mg/L CaCq) 87 87 83 Total Residual Chlorine (mg/L) <0 10 <0 10 <0"10 Temperature CC) 248 24.8 24 8 25"0 25.1 256 24.9 24.6 25. 1 24.3 253 P1H (SU) 809 8 13 804 89 8071 8041 D (mWL) 805 813 7.87 8.5 7.901 7.991 7.95 79 82 84 82 883 8378 821 84 Conductivityftmhos/cm) 88.4 8.1 8.4 209 203 211 202 192 215 200 100% Intake Alkalinity (mgL CaCq) 73 7269 Hardness (mg/L CaCQ) 83 89 Total Residual Chlorine(mg7L) <0 10 <0.10
__ Temperature(C) 25.11 2481 2 24.5 2501 25.11 246 27 250 24.3 252 247
ronmental Testing Solutions, LLC "T'
Species: Pimephalespromelas Client: 5,Pn 3 A Date: A-Of-(AZ.
Full-strengthChemistry: OUTFAALL_ 101 Parameter Sample Number: Control Batch:
1 2 3 01-29 02.05.= .0Z pH PHss~u.S0 (S.U.) 8-10 ~ *1 f " 1 &.0-0 _____ __ __
DO (mg/L)__ _ __ _ __ ___ _ _ _ _
Conductivity 2 11 z 3 03 ___1 (gjnhos/cm) _ _ ____O _ _ _ _ _ ___ _ _ _ _ _ _
Alkalin ity '363 (03 (mg CaCO3/L) I _ __" __ _ _ (, 1o Hardness (mR CaCO3/L) 6?:
Chlorine 4 Io.O
.40. 10 (mg/L)0 Collection start date -- 0-o rz-Collection end time 72.
Grab or Composite z1-&'-POUR U"Vt.k 2-A*Z-4ou_.
(duration) CORf1'P- CO1APO'- CA rt I Temperature (C) upon receipt __ "______ 2_" _ (.1" C_
Physical Pak -tui characteristics di O..oc.w z' Dates sample used O eo-OZ 0-01'*-a o4-O-O6z o0- I.Z-O&.
ETS Project and al t Sample numbers O'oed.o.Oq 0whi.Op cZo&I.oS
ýYuuumental Testin?. Soluitions, LLC page fJ at h umyirriumental Testing Solutions, LLC Page bat S Species: Pimephakespromelas Client: 4Pn~c7?JA)A-NAR~ Date: -Qt -&O-O2-Full-strength Chemistfy: Ii N 4 M~e.
Parameter Sa pe Number: Control Batch:
_______ 1 2 3 0'+4R.Olz neM8 c8.0-t2 pH
- Y DO (nig/L) _____b Conductivity z.. 505 Sol_
(gmnhos/cm) 9_ M_ ___31 Alkalinity qLo (3C 1(mg CaCOIL) _ _ _ _ O ~ I_ (' I_ o ,
Hardness (mg CaCO3 IL) E)ci Ia Chlorine 40104C 40.t)i~O Collection start date **
Collection end time INS3 Grab or Composite ~-zsA*%JVR 2-+ JIb.. Zs-Roo (duration) cd~as~tsr Cd.i-Z QIAfST Temperature C0C) 0 .15 C. OS*-c-.! -C upon receipt_______________
Physical j~QCOW6~ d~e iL JJ characteristics Ckj- t-0io Dates sample used a,(60 o-ofy07- 09-10&0 Lit. tz -OZ LETS Project and lq.20 Sample numbers 10 7O(- CS ow otof. o-7l2Cf
rimnm ental Testing Solutions, LLC Page 7 ot 8 Species: Pimephalespromejas Client: '5' Date: 0-W-L
Species: PimepkakSpromelas Client:
Date: - Ot M Paraxneter v 3 4 pH (S.U) gf. Cn 6 DO (mg/L) 3 -Oz .(Of Conductivity
-Sol a03 Temperature %I (CC) zq -S zl.s -zq.-7 zq -.3 Zq-0 24.5 12H (S.U.) 0-7 - goo -+.I z 8.0 IYO -4 Conducti . .6
)SICM :2 Temperature zq
Species: Ceriodaphniadubia Client: - <,P3 CHAl-A Date: OS- -07~o Full-strenglh Chemistry: 01FLL1 Parameter smle Number: Control Batch:
2 1 .f1-(S.U.) L ______
R.o1 I
-7.qc4 8 -0 DO (Ing/L) ~E-3 lb.26 Conductivity 2181 -4..E (gmhos/cm) 'R1.I e-0 zo I ARkainity (. C (mg CaCO 3IL)
Hardness (mg CaGO 3IL)
Chlorine (MRngL)
C ollection start date c - q o - o -6 0 - 7 Collection end time I~
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Species: CrWdaphniadubia Client: g'eQ,4AIA Date: QF n-07, Full-strengthChemistry: Ja-pj<e Parameter SampieNumber: Control Batch:
1 2 3 61?.2q*Z j06.oSzo0i , 4.O 2 pH DO (ms!L) - ' - '.1.",. m ,
Conductivity Z 3M0
(ýumhos/cm) _ _ _ _ ___- _ _ _ ___ _ _ _
Alkalinity 40 (mg CaCO 3/L) b~a (0 Hardness (mg CaCO 3JL) 9q Chlorine "0. 0 Collection start date aO-06-O7. *.*-OT_
Collection end time I INS lq Q, Grab or Composite 'Z5 -kb-L Z3- to490 Z3ti*'w .
(duration) CklbSme. ca AxrP CDH I'cft' Temperature (oC ) 0 .5 1 I.5'C upon receipt Physical C.a.eL characteristics NO C Ao0.M .
Dates sample used oe0C.- oZ. ot.O-oZ CC-&c6OZ
- o. *-I-OL ETS Project and Sample numbers .Oz 05 . 2IO.-o
Species: Ceriodaphniadubia Client: -*oti)A IA Date:
Species: CerioaPhniadubia Client: 6e IOs4A ,
Date:
Concentration CONTROL Paaee 3- 4.~
PH (S.U.) 5.~ .5 ab. 6 DO (mg/L)
Conductivity 6.2.1lb 6.
(ixmhoscm) d3os 30Z. L Temperature a
PH (S.U)
DO (mg/L) SA .0 Z .0 Conductivity
/0Aq1 (tanhos/cm)
Temperature (OC) VVI 28 *Z.
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DO (mg/L) zc o ZI- q.f Conductivity 72%5 (junhoslcm)
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DO (mgtL)
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Temperature
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Temper I (.C)
Environmental Testing Solutions, LLC Chronic WThole Effluent Toxicity Test (EPA/600/4-91/002, Methods 1000.0)
Species: Pimephalespromelas Summary of Chemical Analyses Clent. Sequoyak Nuclear PlantUV-Trested Test dates: August 06-13, 2002 Project numbera 405 Remewed by. C pimeqhalespromdas 27 241 2511 031 2441 212 2409 031 Overall Temperature CC) 243 25.5 24-9 0.3 (including all concentraton fof nmlaland final temperatures)
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/60014-91/002, Method 1000.0)
Species: Phnephalespromelas Daily Chemical Analyses Client: , Seqi uoyah Nuclear Plant UV-Treated Test dates: Aug ust 06-13, 2002 Project number: 405 Reviewed by: ck Concentration Parameter Da 0 1 Da Da 2 Da 3 1 D 4 Da 5 Da 6
__________________ Initial I Final I Initial Fina Initial I Final I Initial FIna nFinal nl PH (SU) IiFinal inl Inta Final 808 803 808 770 806 765 802 7.70 806 DO m'/l 7.62 8.04 7.70 7. 762 80 83 8.1 79 75 82 76 Conductivity (pmhos/cm) 80 7.1 8.1 7.3 8.1 7.3 305M298 311 Control Alkalinity (mg/L CaCQ) 66 297 63 63 Hardness (mg/L CaCQ) 87 85 89 STemperature (C) 2451 25.1 246 2 44 2485 244 24.7 24.3 246 PH1 (SU) 807 24.6 802 808-774 807 -76 802 7.74 805 8.04 10.98% Cond civt 8.1 83 80 779 75 82 76 80 8.2 8.1 .3 Conductvit mhoscm 306 88 294 296 D C)25.1 8. 25.1 83 "" 24980 250 244 7.98 7.592 246 24.7 244 24.7 248 246 PH (SU) 807 799 808 770 807 7.66 802 66 805 804 7.6 7.98 760 22% 8 82- 74 7.9 83 82 8.2 7.4 81 Conductivit mhos/cm 282 276 294 275 7.3 272 280 281 Temperature CC) 246 25.1 25 pil (SU) 807 244 248 25.48249 252 250 246 802 808 79 806 75 802 76 803 7.7 803 761 7.98 7:54 DO0(mgL) 82 83 80 7.9 7.
Conductivity (pmhos/cm) 269 251 266 256 2 260 100% Temperat -mure 260 CC) 24.8 .25.1. 253 24.7 246 48 24.7 25.2 25.2 PH_____________ 8071 804 808 70 805 25.1 2441 763 801 7661 801 7.6 801 7.63 7.97 7.59 2% 72%eDO Conductivity (mg/L (pmhos/cm)
C 81 234 83 8.2 21238 79 7.5 82 7.3 81 82 7.1 81
-226l 220 22626 7.3 T24 25.1 254 24,8 246 25.1 244 2491 251 254 225.1 ni (SU) 809 802 245 T-7 805 768 802 7.75 7.99 7.64 DO mgIL 7.99 7.
8.2 84 83 78 74 82 Conductivity(pmhos/em) 28010900193 7 8.2 7.2 8.1 787 19619 100% Alkalinity (mgIL CaCO,) 71 69-----
Hardness (mg/L CaCq) 8 87 83 Total Residual Chlorine(mg/L < <0.10 <010 Temperature CC) 247 25 1 254 PH (SU) 249 24.5 25. 4 24.9 1 25.4 8051 _8041 807 7.6 8031 25.21 25.3 24.6 7641 7.75 78 796 7.3 7.961 7621 7.94 DO(mg/L) 81 831 83 7 75 7.91 7.21 -8.2 7. 82 72 82 75 82 Conductivity pmhos/cm) 203 198 204 212 74 190 19519 100% Intake Alkalinity (mIL CaCq) 73 720 695 Ilardness (mgILCaCq) 83798 TotalResidualChlorine(mgILA <0.10<00<.1
________Temperature CC) 1 24 61 2. 5 5 2491 24 51 2501 2441 2461 25.11 25.51 25.11 25.21 2461
e :) u, o Species: Pimephalespromelas Client: Y-r AA -I- U-ITggj-rft, Date: tL-02' Full-strength Chemistry: OVf:L 10 "AganqLA LIc 1-- 1 Sample Number: Control Batch:
I I - I 4 I .
pH S.U.) s.zq 7. '9 8.01 DO (mg/L) R-.0 Conductivity I.2.
(pxnhos/cm) 8.q o09 Z98e Alkalinity (nmg CaCO 3/L)
Hardness (rag CaCO3/L) E. 0ST 8-4 Chlorine ZO.IO (mnz/L) w -J Collection start date Collection end time
-,I7' 0 Grab or Composite (duration) cos*osrr*
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ETS Project and qoz TZOPLo.A Sample numbers I
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Date: Q 5& Fa-=
Full-strength Chemistry: IMT;9Ke Parameter Sam le Number:
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1 2 3 pH 7 S.U. 8 .5 7.?) T'. 09 6.08 0.0o.
DO I.
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( os/cm) 2o3 P10 (o~a5 Zqoc q.
Alkalinity 7:M o3 q"
(mg CaCO 3/L) 143 Hardness (mg CaCO 3/L) 85 SoJo& z.
<*0.10 Collection start date W063-01.
o&O,4-aZ.
Z3140U Collection end time
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characteristics COLO<(
Dates sample used oR-d'7-oZ.. Cop.oA.0 OZO.rP.o1.of'IZ-OL ETS Project and qaS Sample numbers =or*.ol
Page 7 ot 8
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Species: Pimephalespromelas Client: 6e-) V Q 0 frAeAleb Date: flt-cAr-oZ Daily Chemfrtry:
Concentration Parameter012 CONTROL pH oSU)8 F- 90 8 1 0 80o-.(
Conductivity Temperature 16qg'O7, Conductivity Temperature
__0c__ Z4 -S z'kIzq i Conductivity 2-Z5 (pxnhosm :02, 2q Temperature z. ~.
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k~ Conductivity (Pmhos/cm) 5 Temperature z~o z.
32% Conductivity 5 Temperature Z. SI~~
(0c)z RH (S.U.) .0 .43 7.68
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1000 Conductivity (Rimhos/cm) ZO6 I
Temperature ~
(0c) .241 Z5CC) pH (S.U.) 9.- G.0q 604 -1~
1060- DO(mgfL 9)
Conductivity
/AT7Y&Qnhos/cm) Z035N 0 Temperature 2 q5 zq
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IInitial II Fnl IInitial IIFnl iInitial II Final I
Environmental Testing Solutions, LLC Page sat 8 Species: Pimephalespromelas Client: t'S rI q A I "
Date:
261 I
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Environmental Testing Solutions, LLC PP -ag PW e - .of V _
Alkatinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L v~
Analyt Date analyzed [8k.u Titrate samples to pH = 4.50 S.U.
pH of IINornality Deionized Titrant check Er water reference standard 4.5 S.U. number I.n.. I I Reviewed by: Date reviewed: ~0 -
Environmental Testing Solutions, LLC Pageabo SPage _Z ___-of- q Allalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst late analyzed ~I~ z Titrate samples to pH = 4.50 S.U.
Reviewed by: Date reviewed:
Environmenta Testing Solutions, LLC Page 3 Pae :b Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO3/L Analyst Date analyZed Titrate samples to pH = 4.50 S.U.
number Sample ISample
_ Bgn nd Total Multiplier Alkalinity %RPD =
(mg CaCO%/L) f(S - D)/[(S+DY2]} x 100 DupLi , i _.__ ___ (acceptable range -h 10%)
I Sq 14~
9-Reviewed by: j~ J Date reviewed: o.2O-
Environmental Testing Solutions. T.T.i Page II e Alkalinity (EPA Method 310.1)
Matrix: Water, MDL = 1.0 mg CaCO /L 3
Date analyzed 5.6 1 - )2_ Titrate samples to pH = 4.50 S.U.
ntand H/er ddrsudn:
Delonized Tltrant Normality (A)Of H4Sb,4 pH Factor or Multiplier
-BWn I End Total water reference standard mi T-nff- (5 ml Na2CO X0.05ym S(NIX 50000)/ 100 ml sample 4.5 S.U. number number --M!L -NX500 I I L 1. 1 D ie sample precision:
Y - -
Sample Sample Sample ID Alkalinity %RPD =
volume Begin End Total number Multiplier (mC CaCO3/L) {(S - D) /[(S+Dy2J) x 100 Z (ml) ml ml (acceptable rxnge - +/- 10%)
mal 02.
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Reference Spike value Sample standard number (SV) Spike alkalinity (A)
IsS* SO I OL*:*
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volume Ml Begin ml End ml Total Multiplier 2*~u~pe[ (Mg CaCO.pL) m *L ml
Environmental Testing Solutions, LLC Page Page _ . of Total Hardness (EPA Method 130.2)
Matrix: Water, MDL - 1.0 mg CaCO3/L Analyst Date analyzed [j~
Note If >15mI of titrant is used, sample must be diluted. Reviewed by: = ý Date reviewed Ic- 'Oz]
Environmental Testing Solutions. LLC
- Pae
~Page _._.ofo Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO /L Analyst 3 Date analyzed [06.13 ub.ani.no andbmu ftrant Normalety checks reference sftandard mli number num rtber Note: If >l5ml of ttrt is used, sample must be dilute& Reviewed by: --- ] Date reviewed - ]
Environmental Testing Solutions, LLC P Page (VF Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg.CaCO 3/L a analyze Fi-Date analyzed[7I j Hardness %RPD =
(Mg CaCo-J/L) I(S - D) /[(S+D)/2)
- (acceptable range S
W D
Spike hardness (mg CaCO/L) (A)
Rq yD rew Note: If >15ml of ttrant is used. sample must be MIuted.
Reviewed by: [IiIZ Date reviewed ~~~Z
Environmental Testing Solutions, LLC Page 1,0)
Page jofCI:C Total Hardness (EPA Method 130.2)
Matrix: Water, MDL = 1.0 mg CaCO 3/L Analyst Date analyzed 95i]ii Normality check Eed standard 1 ml Endl pH Factor or Multiplier
ý. number Im = (Nx 5000)/5O ml sample
- N 1000 Date reviewed Note If*> 15m of tnnt is used, sample must be diluted. Reviewed by:
Environmental Testing Solutions, LLC Page 1__.j Page of L..
I....L.
Total Residual Chlorine (EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter Accumet Model 25 pH/Ion Meter Analyst Iodide reagent Date anablyed 1 836n7 Acid reagent Slope:
Calibration:
0.10 mg/I., 1.00 mg/L I L Reference standard number I 4&-ýq 1 1 M650q I Note: For samples with a resiulal chlorine of > 1.0 m&g/4, the calibration range must be adjusted to bracket the chlorine levels ofthe samples.
Laboratory controlstandard:
Reference standard True value (TV) Measured value (MV) % RS=MV/TVx 100 number (mgfL) (mgIL) (acceptable range - 90 to 110%)
I0.50 c.,n Duplicatesample preciswin:
Sample Sample ID Sample characteristics Residual chlorine %RPD = {(S - D)/[(S+D)/2]} x 100 number (rg/L) (acceptabl range -: 10%)
Duplicate D Sample measurements:
Sample Sample ID Sample characteristics Residual chlorine number (mg/L)
N Blank (should be = <0.0 mg/L) . -- ; o'o a W08010~. OZ Coeot-8L -PP tan 1-0s 1t'44. Zocol O2OQdyj,.3 Coetrt *CA ~4ian CLOcbc. 'LO.coiO10 o 602000.6/ TP&tt t3etn, e 40.00-46d Note: All samples were analyzed in excess of EPA recommended holding time (15 minutes) unless otherwise noted.
Laboratory control standard:
Reference standard True value (TV) IMeasured value (NM) %RS=MV/TVz100 nubr(mgQL (ingfL) (acceptable range - 90 to 110%)
I )N(.0910.50 O).MLAS(D011 Reviewed by [ .-
Date reviewed
Environmental Testing Solutions, LLC Page 3 Page I of Total Residual Chlorine (EPA Method 330.5)
Matrix- Water, MDL = 0.10 mg/L Meter:. Accumet Model 25 pH/Ion Meter Date ana47zed [I .0711 Iodide rcaget 1p3 b %F167 Acid reagent lI j zRib4 Slope:: iI I I Ca1brat0on: .1o0-,I 1 1.00=nw/
l Rference standard number I
Note: For samples with a residual chlorne of >Refierenc s numbie 1.0 mg/&I the calibraton range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrol standard:
Reference standard True value (TV) Measured value (MV) number % RS = MVI TVx 100 (rag/L) (me/L) (acceptable range -90 to 110%)
Dp 6ce 1 0.50 0 Lon Duplicatesamole precision:
Sample I Sample ID Sample characteristics Residual chlorine %RPD = {(S - D) /[(S+D)/2]} x 100 number m (acceptable range - - 10%)
DuplicateD l
W-lox, 'LO-CaB(1 I
Laboratory controlstandard:
Reference standard True value (TV) Measured value (MV) number % RS = MV / TV x 100 (mg/L) (mg/L) (acceptable range - 90 to 110%)
I Li reviewed0.50 0"
- Reviewed by Date reviewed
Environmental Testing Solutions, LLC Page _5 Page I1 f Total Residual Chlorine T
(EPA Method 330.5)
Matrix: Water, MDL = 0.10 mg/L Meter: Accumet Model 25 pH/Ion Meter Dat anAnalyzely I00- JO -oz.. Iodide reagent:
Acid reagent:
Slope:
Calibration:
0.10 mgWI 1.00 Reference standard number .... I I I m/L Note: For samples with a rsdual chlorine of > 1.0 ing/L, the calhbration range must be adjusted to bracket the chlorine levels of the samples.
Laboratorycontrol standard:
Reference standard True value (TV) - Measured value (MV) % ES = MVI TVx 100 number (mg/L) (mg1L) (acceptable range - 90 to 110%)
/NeA I~q 0.50 o.3 4.5 Duplicatesample precision:
Sample Sample ID Sample characteristics Residual chlorine %RPD = ((S - D) /[(S+D)/2]} x 100 number (rag/L) (acceptable range - 4 10%)
o32 O.' b-tlo mpLC) S O-Ooa6q4 Duplicate r D Sample measurements: .
Sample Sample ID Sample characteristics Residual chlorine number
'* ' <t Blank (should be= <0.10 m -"gL) * £o n.2-5 Q -! a -, L &i 'no ,)-K, i..OlS3, Note: All samples were analyzed in excess of EPA recomnended holding time (15 minutes) unless otherwise noted.
Laborator control standard:
Reference standard True value (TV) Measured value (MV) % RS MV /TV x 100 number (mg/L) (mgiL) (acceptable range - 90 to 110%)
I*N 60T/ 0.50 0o.s53-4 _ _ q Reviewed by I n(f Date reviewed I L .IkTH
Sequoyah Nuclear Plant Biomonitoring September 6-13, 2002 Appendix D Reference Toxicant Test and Control Chart A
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 1.2 I "1I I I J I I I I I. I I I I I Control Limits Set According to +/-2StandardDeviations.
1.0 Q * ° * * '7.* .
z0. 8 Q 0.6
..... .. . . . . . .o. . ... .... .. .. .. .
0.4
, I II _I I I I I !, IS S 0.2 04 date 02 OR aOlts Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population. I Central Tendency (mean IC25)
Control Limits ..(mean IC25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromnelas using Moderately Hard Synthetic Water 2.0 I I I I I I I I I I I I I I i i I I I I Warning and ControlLimits Set According to 75th and 9 0 th PercentileCVs.
1.5 U
1.0 U 0.5 N.=
. o.*..................
- ....... ... o....o....... ° o.....
0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.11) is less than the 75"' and 90"' percentiles determined by the USEPA, these percentiles were used in setting the warning and control limits.
I I I I I I I I I I I I I , o I 0> O' 0-0 ell all Ol a. 0ý- a~
>W" O 'W I II I Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephalesgrowth for the test population.
Central Tendency (mean IC25)
- -- Warning Limits (mean IC25 - SA 75, SA.75 = 0.38, as determined by USEPA for the method and endpoint)
........ Control Limits (mean IC25 +/- SA 90 , S 90 = 0.45, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date 7-day IC25 CT S SA7s Cv (g KCI/L) (g/L KCI) CT-2S CT+2S CT - SA75 CT + SA.75 CT-SA. CT + S.o 1 06-26-01 0.69 2 07-17-01 0.53 0.61 0.11 0.39 0.83 0.23 0.23 0.99 0.27 0.16 1.06 0.18 3 08-21-01 0.74 0.65 0.11 0.44 0.87 0.25 0.27 1.03 0.29 0.20 1.10 0.17 4 09-25-01 0.62 0.65 0.09 0.46 0.83 0.25 0.27 1.03 0.29 0.20 1.10 0.14 5 11-01-01 0.60 0 64 0.08 0.47 0.80 0.24 0.26 1.02 0.29 0.19 1.09 *0.13 6 11-06-01 0.71 0.65 0 08 0.49 0.81 0.25 0.27 1.03 0.29 0.20 1.10 0.12 7 11-27-01 0.66 0 65 0 07. 0.51 0.79 0.25 0.27 1.03 0.29 0.20 1.10 0.11 8 12-12-01 0.57 0.64 0.07 0.50 0.78 0.24 0.26 1.02 0.29 0.19 1.09 0.11 9 01-04-02 0.63 0.64 0.07 0.51 0.77 0.24 0.26 1.02 0.29 0.19 1.09 0.10 10 02-05-02 0.49 0.62 0.08 0.47 0.78 0.24 0.24 1.00 0.28 0.17 1.07 0.13 11 02-13-02 0.61 0.62 0.08 0.47 0.77 0.24 0.24 1.00 0.28 0.17 1.07 0.12 12 02-26-02 0.65 0.63 0.07 0.48 0.77 0.24 0.25 1.01 0.28 0.18 1.08 0.12 13 03-28-02 0.56 0.62 0.07 0.48 0.76 0.24 0.24 1.00 0.28 0.17 1.07 0.12 14 04-23-02 0.70 0.63 0.07 0.48 0.77 0.24 0.25 1.01 0.28 0.18 1.08 0.12 15 05-07-02 0.61 0.63 0.07 0.49 0.77 0.24 0.25 1.01 0.28 0.18 1.08 0.11 16 05-23-02 0.76 0.63 008 0.48 0.78 0.24 0.25 1.01 0.29 0.18 1.08 0.12 17 05-24-02 0.66 0 64 0 07 0.49 0.78 0.24 0.26 1.02 0.29 0.19 1.09 0.12 18 06-04-02 0.67 0.64 0.07 0.49 0.78 0.24 0.26 1.02 0.29 0.19 1.09 0.11 19 06-11-02 0.70 0.64 0.07 0.50 0.78 0.24 0.26 1.02 0.29 0.19 1.09 0.11 20 08-06-02 0.55 0.64 0.07 0.49 0.78 0.24 0.26 1.02 0.29 0.19 1.09 0.11 Note: 7-d IC25 = 7-day 25% inhibition concentration. An estimation of the concentration of potassium chloride that would cause a 25% reduction in Pimephales growth for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
SA.S = Standard deviation corresponding to'the the 75th percentile CV. SA.75 = 0.38, as determined by USEPA for the method and endpoint.
SA.90 Standard deviation corresponding to the the 90"' percentile CV. SA90 0.45, as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values.
USEPA 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R.00-.003. US Environmmental Protection Agency, Cincinnati, Oll OirgantswL obtainedfrom Aquatic BMS),stems, Inc 08-06-02.xls
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Control Chart for Pimephalespromelas using Moderately Hard Synthetic Water 40 1:ý 30 S20 10 0
Test date
-- PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
I _Central Tendency (mean PMSD)
........ Lower and Upper PMSD Bounds Lower PMSD Bound (10th percentile) = 9.4%, Upper PMSD Bound (90th percentile)= 35%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Envirofnmental Testing Solutions, LLC Precision of Endpoint Measurements Potassium Chloride Chronic Reference Toxicant Data for Pimephalespromelas using Moderately Hard Synthetic Water Test number Test date Control Control Mean Survival Cv CT MSD PMSD CT Growth
(%) %(Mt Vae) (%) for Control Growth CV (%) (%) for PMSD (%)
1 06-26-01 100 0.63 9.8 0.19 30.6 2 07-17-01 100 0.52 9.1 9.4 0.07 13.8 22.2 3 08-21-01 100 0.89 8.3 9.1 0.13 15.1 19.9 4 09-25-01 100 0.85 4.9 8.0 0.09 10.4 17.5 5 11-01-01 100 0.54 2.5 6.9 0.10 18.6 17.7 6 11-06-01 100 1.00 7.4 7.0 0.17 16.9 17.6 7 11-27-01 97.5 0.70 22.8 9.3 0.18 26.1 18.8 8 12-12-01 95 0.82 16.6 10.2 0.19 23.5 19.4 9 01-04-02 95 0.80 10.1 10.2 0.18 22.0 19.7 10 02-05-02 97.5 0.95 6.9 9.8 0.21 22.2 19.9 11 02-13-02 100 0.65 8.0 9.7 0.10 15.5 19.5 12 02-26-02 100 0.64 3.3 9.1 0.11 16.5 19.3 13 03-28-02 100 0.63 11.2 9.3 0.13 21.4 19.4 14 04-23-02 100 0.71 12.8 9.5 0.19 26.2 19.9 15 05-07-02 97.5 0.46 12.3 9.7 0.07 15.4 19.6 16 05-23-02 100 0.38 9.9 9.7 0.09 22.5 19.8 17 05-24-02 97.5 0.44 21.3 10.4 0.11 25.0 20.1 18 06-04-02 100 0.68 140 10.6 0.15 22.1 20.2 19 06-11-02 100 0.65 3.7 10.3 16.6 0.11 20.0 20 08-06-02 97.5 0.69 22.2 10.8 0.16 23.5 20.2 Note CV = Coefficient of vanation for control survival On average, the CV for control growth is 10.8% in Environmental Testing Solutions, LLC Pimephales chronic toxicity tests.
Lower CV bound determined by USEPA (lOn' percentile) = 3 5%
Upper CV bound determined by USEPA (90+/-h percentile) = 20%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mminmum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Punephalesgrowth by 20.2% from the control.
Lower PMSD bound determined by USEPA (101 percentile) = 94%.
Upper PMSD bound determined by USEPA (90'b percentile) = 350/.
CT = Central Tendancy (mean Control Growth CV or mean PMSD)
The lower and upper bounds were calculated by the US EPA using 205 tests conducted from 19 laboratories for Pimephalet growth in chronic reference toxicant tests USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-0O-003. US Environmental Protection Agency, Cincinnati, OH.
Organsmsobtainedfrom Aquanc BioSytems. Inc= 08-06-02.xls
-... *,L~utCnUU Lesnng Solutions, LLC Page I ot 5 uuuscuraz testing Solutions, LLC Page 1 at 5 Potassium Chloride Chronic Reference Toxicant Test (EPA/600/4-91/002 Method 1000.0)
Species: Pimephalespromelas PpKCICR Test Number. Z9 Dilutionpreparationin ormation: Comments:
KC1 CHM number: CK061,_
Stock preparation: 50 gKC/21 Dissolve 50 g KCI in I-L Deionizedwater Ddut= prep(mWgL) 300 450 600 750 900 Stock volume (mL) 6 9 12 15 18 Diuent volume (mL) 994 991 988 985 982 Test organism information: Test information:
Organism age: 24-Aoues ot.0 Randomizing template: "_t.oLQ.A Date and times organisms O&-o*-2. jos -m t"b Incubator number:
were born between: A_2-r"I Organism source: AIS PA
- C.O-S-O2.. Artemia lot number: g-27_!
Transfer bowl information: pH1 Temperature = Oven temperature: IC)\"
I .____2q. R'C Drying time: Iq- V1; Dailyfeeding and renewal information:
Day Date Morning feeding Afternoon feeding Test initiation, renewal, Analyst time time or termination time 0 ot0.,p ______I_(1_2___
___ 08-cýnl072. 1060'61 3
3 /-11-O7L- 0 ?-~k~ 1000..
_____ 2 iic41 I- q 1si65 CA '
5 c*-gi-oz, iooo tro.>,si' 0*
6 !na-, I 1622 _ _ 6__5__._
Op 1~-3-Q2 _____ (_____
z____
Controlinformation: Aceptance Cnteria Summary of test endpoints:
% Mortality: 1 2.57o .52o% 7-day LCS0 O.3 Average weigt prlarvae: o.4-4 0.25 mearva NOEC _ _ _0 I LOEC (00 ChV 5m. C IC25 :5:52 -(
tnvironmental Testing Solutions, LLC Page 2 at 5 Luvironmental Testing Solutions, LLC Page 2 0+/- 5 PpKCICR Test Number: ._Z3 Survival and Growth Data I Day CONTROL 300 mg KCIIL 450 mg KCI/L A B C D E F G H I J K L 0 /0 to 1/ 0/o 0/0 o /0 /0/0 /1/0 12 1 /0(0 to 1/0 /0/0 /0 /0 /0 /0 /0 2 /0D /) I / /I0 /0 ////0 I /0 /0 3 t0 io/ (0 10 /o
/01 /o 1/0 /0 /o /0 4 1010 o0 /0 t0o 0 /0 (o I/ to 10 10
_ I
/0 0 10 V4 I/10 (ao 10 [(0 /0 16 6 10 0 t( 9 ' I )6 1'"0t o 10 7
7o (0 o lb 10 9 7 io /0 1t ,O /0 Pan + Larvae weightr(mg)
- 2 - Vj's ___ 8vewe.
j1 '
Larvaeweight(mg)-A-B . 1 &' '5 10A .81;t Calculations and data reviewed.
Comments:
I E-'
'o
-- - -o 1:%C 3: .
N.. ! . ~
- 1~1 _
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1000.0)
Species: Pimephalespromelas Quality Control Verification of Data Entry, Calculations, and Statistical Analyses Test number: PpKCICR # 29 Test dates: August 6-13, 2002 Dunnett's MSD value: 0.1633 MSD = Minimum Significant Diflerence PMSD: 23.5 PMSD - Percent Minmnum Significant Difference PMSD is a measure of test precision. The PMSD is the inimum percnt dilrnwnce between theOMMnb'th*tila imet can declared statistically significant in a whole effluent toxicity test. On average, a significant differaes. occur fora Testing Solutions, LLC chronic toxicity tesLs when a toxicant reduces Punephales growth by 20 2% from the connoi (dstuminnd Lower PMSD bound detennined by USEPA (10th percentile) = 9 4%.
Upper PMSD bound determined by USEPA (90th percentile) - 35%
"lhe lower and upper bounds were calculated by the USEPA using 205 tests conducted from 19 laboratories for PEkaes growth In chronic reference toxicant tests.
USEPA. 2000 Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-433-R-00-003. US Environmental Protection Agency. Cincinnati, OIl Organtrmr obtainedfrom Aquatic BjoSystenu. Inc 08-06-02 xal
N-Environmental Testing Solutions, LLC Statistical Analyses San DW 0&/602 Trot Ia PP.CIC F Ct h sad Ssrt4el EndDM 111/02 m Lmb 0m n> ETS-e . e S p -IG .tT w TU - e ,p K .w40,* 0= dd ,.
Cems-mt. 14 0-Coesl 0.7640 0.4867 0.63I2 0l.455 300 0ai0 0.7627 0.7615 0.77'6 450 0.8170 0.9940 0.71 0.7337 600 M 0.696 0.4319 0.3727 750 0.M 0J.37 0.4160 (2706 900 0.0670 000 0.0472 0.0997 TM Ceso-/2. s N-M MeMs Tr Usm&ares Mk max M N 1-Ted M DCeevl Cr'dld- hD Mesa l----- a 06944 2.D 0.6944 0.4867 0..,49. 22...94 4 300 0.7764 21121 0.7764 0. iiX6M 0.7615 11106 2.643 4 450 0.716. , 20329 -!150 2290 0-2633 0.7354 0.7262 0.5940 0170 10000
- 600 12.867 4 4306 0.475S 0.6843 0.4753 2.290 0.1633 0.7162 0.3717 0%%696 I29s9 0.9740 750 0.3302 4 3068 2.290 0.4756 0.3302 01633 047S5 0.6466 900 0.0536 0.2706 00772 0.0536 0.0000 004160 0997 20641 77.723 4 0.3302 0.4490 00536 aO729 AfUtt' TentCetu st K&
B.M.W1TtT09623346911 leg
[.&- -I ..-- - - .06)7.23604484 Ilypoeshs Test (-o.l. 0.0L3 0.844 40.638576412 0.709160105 NOEC LOEC Chy 11.344U3201 Du.mts Test TU MSDu M3 MSDp 450 600 519 61324Z3 MpSE F-?r.6 df 0.163333371 0.23*Z39247 0069053993 0 z02017025 0006295148 3.12 point mt/L Un te'pewaos tttpeez) C0 RI SID 9% CUEtp)
IC05 Skt.m 46098 5964 2i217 492.12 -I.3622
[CI0 483.89 35 10 30899 52700 .1.5212 IC25 506.80 28.29 374.27 567.34 -2 0268 IC20 S2972 2703 10 433.2 60672 .0.3757 IC25 55263 09 27.25 464 LI 63977 IC40 00988 63540 34.32 53413 72398 0.0431
[C50 0.8 711.31 3208 590.57 79473 -04304 07 So06 0.5 04 0.3 0.2, 0.2 001
-0 .
0 2o0 400 600 800 1000 Dose IriSL Doe-Res ponse plot 09 08.
07.
006 S05. ..... ............................ . .................... 005 level Sof stgnfi~Cnce o04
- " 0 0,.3 0-2 01 0
O-rgnsrmj obetedfrom Aqu~Cu SBc~ws em. In,"
08-06-02.xls
Environmental Testing Solutions, LLC Statistical Analyses Larvil Fhk Grwnh sad Sunl Tns-7 Day Sarvival Stat DUK /*26 TVonD" PpIKCICR Swroo Mr l- Taaa End Dean: Vl3 Lab ID ETS-Ezw Tenug Solumou SaMD TyW XCL-rD aiNM ch l~tde S mPtseocol: EPAP 91-V0A *rmbw Ti Sposmn P"P-mephi Fmdau Cw-ou/L 1 2 3 4 D.Co l so0000 0000 1,i 0.9000 300 0.90= JA 10000 1AW 450 1.0=0 090 100 L= 00L 600 0.9000 0Ow 0.9000 0.6m) 750 .
I4000 0.700 01000 0.4000 900 0.1000 0.0000 0.1000 0.1000
.1 Tnessform AresJ Squats Root R&sk I-Traled Nusuber Total Coat-qL Me" Ms NMs
- Meas Mas Mat CV% N Sam Crcdw R-asp Noalber D-Contcol 0.9750 10000 1.3713 14490 14120 3942 4 t 40 300 0.9750 1 000 1.3713 1.2490 14120 3942 4 I&O 10000 1 40 450 0.9750 10000 1.3713 1.2490 1.4120 5.942 4 1900 10.00 3 40 600 0.05.0 07IS 311990 0.8631 14120 IL543 4 13.50 1000 6 40
- 750 0.5m 0.597 08669 06147 1.1071 24377 4 1000 10.00 17 40
"*900 00750 0.0769 0.2aI0 0 I36 0.3211 20997 4 1000 10.00 37 40 Auxiliary Tests S$listle Crftical Skew Kart Shapro.W*Iks Teat utru es ,,-naoctialdssmbon (p -001) 0 960099541 0094 -0.553246613 0.623562269 Bardects Test mcbmtesceul vaes a (p - 0 19) 7 398194776 1509631706 Hyiesis Test (l-ail. G.03 NOEC LOFC Chv TV Scad's Many-One Rink Test 600 750 6700203932 Maiximmu lkeftod-Frobit Parmeter Value SE 93% Fiducial Umits Contr8ol Crlticl P-,ale. Ma Sl~m Ite" Slope 1436246231 2.3275*691 9339D35170 1906506944 0023 2"81270389 7814724922 0.42 2.873220573 0067203602 14 k3uIxemp -37 7321S74 7-6266S248 -SL36360157 -231.0203324 "r$CR 00339099 0016532024 0.00k587133 0066392667 10 Pewst Probits sq/I 95% IF1dscl Llamia 2.674 5232334309 4266393214 581.2970711 09 ECO1 EC05 3355 58149a3979 490 8924523 630.7147020 08 ECI0 3710 615 167196 541 0106061 6594439001 07 ECIS 3964 6389775564 572.4799029 67993293.31 WC20 ECZS5 4 350 658.5560426 5977636903 69704031340.06 4.326 6758315673 6200069934 732.460673 a
- 04 OC 06 0
EC4O 4747 721.397-266 6773533936 755.5406079 EC5O 5000 750.2751705 71l.5412693 70533I1776 EC60 5253 7003091207 744323194Z 920.7504566 03 EC75 3674 832.918316 7946955439 3906099305 02 EC9O 59412054 76r2606 0336030.43 922.224542 ECZ3 EC9D EC91 6.036 0809587041 835.2417557 96162433 6202 9350566243 862-21655710o4*5*002
&645 9600376377 902.3614912 t100974L95 01 00 1 10 100 1 1000 10000 EC99 7326 1075334983 9001440071 1206162925 Cose mg/L DOWRfspDase Plot 09 08 07 S05; 04 03ý 02 0.1 Organums obtainedfromAquanc BiSystems, Inc. 08-06-.02.xts
Environmental Testine Solutions, LLC . -t,- . -- .
I PpKCICR Test Number. 29 MHS Control Chemistry:
Parameter Control Batch:
________o'-z'+0-. Mo-S0,2 c.7-o- ?_
pH (S.U.) - I.c6.o1 _ , _
DO (mg/L)
Conductivity (gnhos/cm) I" \
Alkalinity t~ 1ý (mg CaCOVL)
Hardness (mg CaCO3/L) I Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the condctivity falls outside the acceptable range)
CONTROL 280 - 360 300 mg KCI/L 840 - 890 450 mg KCI1L 1100-1180 600 mg KCUL 1390 - 1460 750 mg KCLIL 1660 - 1710 900 mg KCIIL 1890- 1950 KCI Stock 67000 - 76000 (25 g KCMIL)
nvironmental Testing Solutions, LLC PpKCICR Test Number.
Parameter 0 1 2 Concentration CONTROL DO(S.U.) .0 .Z e. 1 370 5 797
).0 ;m.alL I G.z )5.... 1 *l 1 l.
DO
(,hs/cm)5 '
Conductivity (0c) z* q15 Z-.*.7- -24.1 7-4.9 pH (S.U.) *I-'** ns -* 1.Z**L*
I.91
~2. G*1.Z i.'I .O.2 qc DO (m./L)
Temperature (gmhos/cmn) 3 *"- q Temperature V .2 4. 2.
PH (S. ) -7.9 L.O 450 600 mg KCIIL KCIIL Conductivity Conductivity q os/cm) 0 Temperature 2q3.
600 mg KCI/L C(c)
Conductivity 4.
- -*-r . s.7 z -7 T_. n. ._
________ (CC)
Temperature 2-q -3 q q ** 2-Zq .Z*"
TDO (mg1L 8.0 &.S 6-2: "*.9 e.-I
- 750 mg KCIIL Conductivity VV4 15q 10 Temperature z . q 6 21, (0c) . .7 2 .z. 2 .- .
- I.,'8* **pt(..ConductivityG
[.0" ."i "r,
"*lq{ t'O 900 mg KCI/L i(tno/r)OmO (gno/m t1 Temperature z ,7 Zq- 5.. 2 .
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I UILLIM z , z . ..
- Initial k
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PpKCICR,Test Numiber~ :29.
I Concentration Parameter356 CONTROL pH (S.U.) 9. 0 187 B.0( 4U G., .
300KCVLConductivity m
Temperature PH (S.U.) E.13 -4 .,I (a
-DO mg ) B.3 -7.54I(
S 5 8 400 mg KCIJL Conductivity Temperature -z-q. L Z z.a-z~
600gKCILDO (mg/) 8.3 ~ 2 3 450 g KI/L Conductivity Temperature 2q.( ,..r4P Sz 2 . q PH(S.U.) 4.Q 119.16-T 5 8.t
.. Th 1--0 z.
ýe1 7 7q. .
60gK IL DO (mg/ ) -:?.
750g KIIL Conductivity Temperatureaqc q 2 9 &1 2 Z 2q 3 qq 900 mg KCIIL Conductivity Temperature Zq.s TqL V,] z. ~.
__0c___ LCC.)2~
' q q iiuriai ii linal I Initial II 'Funu1 Fia Finn' I2 Lh...1 wU4 I initiaz FIin.I
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Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 1.50 1.25 Q
1.00 tf Q
0.75 0.50 Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC25)
....... Control Limits (mean IC25 +/- 2 Standard Deviations)
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 2.5 Si i i i [ I I I I I I I I I I I I I 2.0
- Warning and ControlLimits Set According to 75th and 901h Percentile CVs.
S................ . ........... .. .. . .... .... ... .. ...... ..................... ............................................ ...... ..... °o.................
- z% 1.5 ..................... ...... ......
1.0 I
U- 0.5 0.0 Since the coefficient of variation for the set of 7-d IC25 values (0.04) is less than the 75 th and 90th percentiles determined by the USEPA, these percentiles were used in setting the warning and contml limits.
I I I I I I I I I I I I I I I I I !
Test date 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphniareproduction for the test population.
Central Tendency (mean IC25)
Warning Limits (mean IC25 . SA 7 S, SA S7 = 0.45, as determined by USEPA for the method and endpoint)
Control Limits ....... (mean IC25 +/- SA , S^90 = 0.62, as determined by USEPA for the method and endpoint)
Environmental Testing Solutions, LLC Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water Test number Test date 7-d IC25 CT S SA-75 SA." Cv (g/L NaCJ) (g/L NaCI) CT - 2S CT + 2S CT - SA.75 CT + SA.75 CT- SAMe CT + SAL" 1 01-09-01 0 95 2 02-06-01 1.11 1 03 0.11 081 1.25 0.46 0.58 1.48 0.64 0.41 1.65 0.11 3 03-07-01 1.04 1 03 008 088 1.19 0.47 0.58 1.48 0.64 0.41 1.65 008 4 04-04-01 1 02 1 03 007 0 90 1.16 0.46 4
058 1.48 0.64 0.41 1.65 0.06 5 05-09-01 1.10 1 05 0 06 0.92 1.17 0.47 0.60 1.50 0.65 0.43 1.67 0.06 6 06-06-01 1.07 1 05 0 06 0 93 1.17 047 0.60 1.50 0.65; 0.43 1.67 0.06 7 07-10-01 1.05 1.05 005 0 94 1.16 0.47 0 60 1.50 0.65 0.43 1.67 0.05 8 08-08-01 1.02 1.05 0.05 0 94 1.15 047 060 1.50 0 65 0.43 1.67 0.05 9 09-12-01 1 02 1.04 0.05 0.95 1.14 047 0.59 1.49 0.65 0.42 1.66 0.05 10 10-10-01 1.03 1.04 0.05 0.95 1.13 047 0.59 1.49 0.65 0.42 1.66 0.04 11 11-01-01 1.00 1.04 0.05 0.95 1.13 0.47 0.59 1.49 0.64 0.42 1.66 0.04 12 12-05-01 1.05 1.04 0 04 095 1.13 0.47 0,59 1.49 0.64 0.42 1.66 0.04 13 01-09-02 0.91 1.03 005 0.92 1.14 046 0.58 1.48 0.64 0.41 1.65 0.05 14 02-05-02 1.07 1.03 0.05 0 92 1.14 0.46 0.58 1.48 0.64 0.41 1.65 0.05 15 03-19-02 1.03 1.03 005 093 1.14 0.46 0.58 1.48 064 0.41 1.65 0.05 16 04-09-02 1.03 1.03 0.05 0.93 1.13 046 0.58 1.48 0.64 0.41 1.65 0.05 17 05-07-02 1.05 1.03 0 05 0 94 1.13 0.47 0.58 1.48 0.64 0.41 1.65 0.05 18 06-04-02 1.06 1.04 005 0.94 1.13 047 0.59 1.49 0.64 0.42 1.66 0.05 19 07-09-02 1.03 1.04 0 05, 0.94 1.13 0.47 0.59 1.49 0.64 0.42 1.66 004 20 08-06-02 1.05 1.04 0.05 0.94 0.42 1.13 0.47 0.59 1.49 0.64 1.66 0.04 Note: 7-d IC25 = 7-day 25% inhibition concentration An estimation of the concentration of sodium chloride that would cause a 25% reduction in Cerlodaphnia reproduction for the test population.
CT = Central tendency (mean IC25).
S = Standard deviation of the IC25 values.
SA.7S = Standard deviation corresponding to the the 75 hpercentile CV. SA.7s = 0.45, as determined by USEPA for the method and endpoint.
SA 9O= Standard deviation corresponding to the the 9 0th percentile CV. SA 90 = 0 62, as determined by the USEPA for the method and endpoint.
CV = Coefficient of variation of the IC25 values USEPA 2000. Understanding and Accounting for Method Variability inWhole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, Oil 08-06-02.xfr
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Control Chart for Ceriodaphniadubia using Moderately Hard Synthetic Water 50 45 40
. ......... .......... .. ... .... ............................................. *..... ,.... .o....°.....,.°o.°.... ..
35 S30 S25
~20 15 5
002 O R) a% ~ 0 0 -1 Test date
- -PMSD = percent minimum significant difference. PMSD is the minimum significant difference between the control and treatment that can be declared statistically significant.
S- -Central Tendency (mean PMSD)
......... Lower and Upper PMSD Bounds Lower PMSD Bound (10' percentile) = 11%, Upper PMSD Bound (90' percentile) = 37%
(Lower and upper PMSD bounds were determined by USEPA for the method and endpoint.)
Environmental Testing Solutions, LLC Precision of Endpoint Measurements Sodium Chloride Chronic Reference Toxicant Data for Ceriodaphniadubia using Moderately Hard Synthetic Water Test Control Control Mean number Test date Survival Reproduction Cv CT MSD PMSD CT for Control (0/) (offWling/female) () Reproduction (¶/) for PMSD (%)
CV (/.)
1 01-09-01 100 28.0 8.4 3.5 12.4 2 02-06-01 1O00 22.1 10.1 9.3 3.1 14.2 13.3 3 03-07-01 100 24.0 8.3 8.9 2.4 10.1 12.2 4 04-04-01 100 25.'0 10.0 9.2 2.9 11.8 12.1 5 05-09-01 100 27.9 8.9 9.1 3.1 11.0 11.9 6 06-06-01 100 27.8 5.8 8.6 3.5 12.5 12.0 7 07-10-01 100 25.0 9.4 8.7 2.8 11.2 11.9 8 08-08-01 100 30.9 2.4 7.9 2.7 8.7 11.5 9 09-12-01 100 28.6 6.6 7.8 2.7 9.4 11.3 10 10-10-01 100 24.9 8.8 7.9 2.5 9.9 11.1 11 11-01-01 100 27.0 6.5 7.7 3.1 11.6 11.2 12 12-05-01 100 24.8 9.1 7.8 4.8 19.5 11.9 13 01-09-02 100 27.5 7.5 7.8 4.5 163 12.2 14 02-05-02 100 23.1 10.1 8.0 2.6 11.1 12.1 15 03-19-02 100 26.3 86 8.0 3.0 11.2 12.1 16 04-09-02 100 26.2 12.3 8.3 2.8 10.7 12.0 17 05-07-02 100 27.3 8.1 8.3 2.3 8.4 11.8 18 06-04-02 100 26.0 9.9 8.4 3.8 14.7 11.9 19 07-09-02 100 29.5 8.0 8.4 3.5 11.7 11.9 20 08-06-02 100 28.4 104 9.5 11.8 8.5 2.7 Note CV = Coefficient of variation for control reproduction.
On average, the CV for control reproduction is 8.5% in Environmental Testing Solutions, LLC Lower CV bound determined by US EPA (I0e percentile)
= 8 91/6 Upper CV bound determined by USEPA ( 'bpercentile) 90 = 42%
MSD = Minimum Significant Difference PMSD = Percent Minimum Significant Difference PMSD is a measure of test precision. The PMSD is the mmnmum percent difference between the control and treatment that can be declared statfstically significant in a whole effluent toxicity test. On average, a significant difference occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Cenodaphrnareproduction by 11.8% from the controL Lower PMSD bound determined by USEPA ( h percentile) 10 = 110.
Upper PMSD bound determined by USEPA ( 0e percentile) 9 = 37/.
CT = Central Tendancy (mean Control Reproduction CV or mean PMSD)
The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Cenodaphniareproduction in chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under Pollutant Discharge Elumination Program EPA-833-R-O0-003. the National US Environmental Protection Agency, Cincinnati, OH.
08-06-02 xls
zriwronmental Testing Solutions, LLC Page Ioat 7 LnlomntlTsin Sou"os LL Pae1o Sodium Chloride Chronic Reference Toxicant Test (EPA/600/4-911002 Method 1002.0)
Species: Ceriodaphniadubia Time Analyst CdNaC 1CR Test Number: 7S Test organism information: Test information:
Organism age: Zq-tkovc 6t.,Ti Randomizing template: GCigj' Date and times organisms c O*-c'-OZ tSZ*TO Incubator number:
w ere born between: 1 '1 _batch: _ _ _ _7.
Organism source: o1i0Q- A' YCTbatch: A6* !1 -O Transfer bowl information: _pH =,CS0 Temperature (OC)=C*'1 Selenastrum batch: ASS ON-lO2 CONTROL Survival and Reproduction Data Replicate number Day 1 2 3 4 5 6 7 8 9 10 1Young produced [) 9 0 c Adult mortality L ... I ._. L ....
_ U L-1-4 ___ __
2 Young produced .....
Adult mortalityL II=:]
Adult mortality 14 tLJ L__ I I- == L. PE~ %-U 4
5 IAdult Youngprouced mortality Young produced L0 Ll L-L4
-41GII
ýL_~ t-( f(W4 HII~
L--
U. __
__ Adult mortality L- L 7 Y-oungprd ue 13 15 /"1 II z.
Total young produced 271 M 2 Z 2(a 32- Z .5 Z Final Adult Mortality L-
- L.- I.- L- T. I X for 3'Broods X - -. _
Calculations and data reviewed:
Test Renewal, Feeding, and Randotmzatuon Record Label Mon Tue Wed Thu Fri Sat Sun Date QLu otLOl. aof-7 &0! d A-. a-Shelf * ' -.
Location Control information: Acceptanct cntena Summary of test endpoints (mgN*vacL):
%of Male Adults: S20o 2% 7-day LC50 _ ._______
%Adults having 3 Broods: 0% 80% NOEC i
%Mortality: 0 q 520% LOEC 11-00 Mean Offspring/Female: 2,. I 150 offspring/female ChV tms.q
%CV: 10. q I <40o% IC25 -to*;4 .
Jinvironmental Testing Solutions, LLC Page 2 ot 7 CdNaCICR Test Number: 2S 600 mg NaCi/L Survial andReproducionData Revlic number My , , 1 -2 3 4 5 6 7 1 9 10 I Young produced 0 j_
Adult mortwality t J ?~. t 3 -
Young dlmrtlt produced - L-
%- L-- -- L- - .
.1 3 Adult produced Young mortality 0A 4
.4 I
5 Adultmortal*ty Young produced 10 4 I d[ L - 1 s 0 -7 L.0 6 Ad-ltmorta*lty Young produced ), -
L- 1,.
L. U.
Adult mortality L L L - U ~ U ~ . U 6 Young produced 19 IS 13 0 16 ... -z Totalyoung produced __ 13 2,1 25 -( 3) 2Z(o ze 2 1 Final Adult Mortality c..... 7L L-. - -
Concentration:
% Mortality: 0%
Mean Offspring/Female: ;2T.
% Reduction from Control: ~-0-. LT%-
Calculations and data reviewed:
800 m NaCI/L Survival and ReproductionData
- Replicate number' Day 1 2 3 4_ -1T 5T 6 7 8 9 10 I Young produced 0 r") r ___.__ ___
ZAdu t mor taii ty -k . __ __ _ _ .
3 4 Youmg produced Young produced Adultmortality
___Z5 L- I.L-0I L4 I I I ___
_I_'S Z I _ _I " 'S ____I Lk -311%
Adultmortality Adult mortality I I
IL--
_L- k.- jl L ° -
6j Young produced C) - 4C jj73 7
- IL 1 - I Total young produced f2 29 12 33
- Z-f *-.2 ZIP 3 2-. 2,5 Final Adult Mortalit k.... L T_-T?---1 -= C. T ? {
Concentration:
%Mortality: 07
.Mean Offspring/Female:_ ZS."/
%Reduction from Control: - 1.1 Calculations and data reviewed: 6-L
N.C1-Environmental Testiniy Solutions- T.TI
-v
... ,..Page
. ...... , 3 at 7 k'age 3 or7 CdNaCICR Test Nuber,.Z 1000 me NaCI/L Survival andReproductionData 6 Young produced 1 AdIu tMartoalty Rlfie umber [
produced O_
. MeYnaf AdCcltoortansy 4f 2onpoue 11 4 5 6 and 7 dt rved V-o Adult m"ortality j SL , t ..
5 Young produced 5 r 7 Young produced
",,7-Total young produced Final Adult Mortality 1 Young produced C C C 2 Young produced Concentration:
3 Youngproduce---'-d 0 C-) o: 0 0 a,., C
% Mortality: O 4 Yount producedG Mean Wfspring/Female: .t
% Reduction from Contrl:t;"FF Calculations and data reviewed: _
1200 6
mgYoung NaC1/L Survival andReproductionData produced Day .9 2 3-_ R I b 1 1
[Adult mortality 2 Young produced 3 Young produce
( 0 0* 0 _0 Adl m'°rtaf'ItylL i Toa Young produced FmAdult mrat Concentration:
%Mortality: 0I Mean Offspring/Female: _aS I,%Reduction from Control: "13, -,7.
Calculations and data reviewed:
Environmental Testing Solutions. LLC to tit 7 CdNaCICR, Tcet Number: Z.S 1400 mg NaCV/L Survival and Revroduction Data m
Day 1 2 3 4 1RepUIc-te nu 6 ber 7 9 9 10 Adul morta.1ty SYoung produced Ic 111I1 o Il T I o o 3
Adultmortality Young produced -- L-VJIL-KI.IL 0mo ,0 l 8011,-
1° j LL. L.
UI 0%
4 Adult mortality +-- - IT 1
lLI*I ,. I T~uJmU* T*I£I** .
4 Young produced Q 0 I 0 0c" 0 6 young
___Adult mortality produced I-Loi - C.I 0 0 -I- 0I 0 I 1 C
.J~f 7 Young produced [ -0*-~ 0 -0 Final Adult Mortality 0 0 0 o C Concentration:
% Mortality: 207.
Mean Offspring/Female: 6
% Reduction from Control:
Calculations and data reviewed:
Environmental Testing Solutions, LLC Chronic Whole Effluent Toxicity Test (EPA/600/4-91/002, Method 1002.0)
Species: Ceriodaphnla dubia Quality Control Verification of Data Entry, Calculations, and Statistical Analyses "Test number: CdNaCICR #25 Dunnett's MSI) value: 2.687 MSD - Minimum Significant Difference PMSD: 9.5 PMSD - Percent Minimnum Significant Difference PMSD is a measure of test precision The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test. On average, a significant difflerence occurs for Environmental Testing Solutions, LLC chronic toxicity tests when a toxicant reduces Cenodaphnia reproduction by 11.8% from the control.
Lower PMSD bound determined by USEPA ( 0'b percentile) 1*a%.
1 Upper PMSD bound determined by USEPA ( 'h percentile) = 37%.
90 The lower and upper bounds were calculated by the USEPA using 393 tests conducted from 33 laboratories for Ceriodaphn/ia rproduction in chronic reference toxicant tests USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R.O0-003. US Environmental Protection Agency, Cincinnati, O11 I
Environmental Testing Solutions, LLC Statistical Analyses CniodaPlml Swrvhdsand Reproductina Tex$Reprodvndiou Setn Doiw V*I2 Tea ID, , CSNCPte am* wot IDS .R Toxkase Fad D 3113102 Lab ID- ETS-Eav. T'amil Sohmons San* Type NACL-Sodoim chlorde Smple Dow Protocol EPAF 91-EPA Prehwater Test Speon CDCaiodpbnA debt Conmmena Cowmg/L 1. 2 3 4 $ 6 7 1 9 10 D.Control 27 000 29000 29000 30.000 22.000 26 000 32.000 29.000 32.000 23.000 600 29 00 32.000 31.000 27.000 25.000 30.000 30 000 26.000 28000 27.000 800 22000 23000 23.000 33000 28.000 23.000 29.000 23000 32.000 25 00 1000 26000 22.000 30000 31.000 26000 24000 23.000 28000 25.000 26000 1200 5.000 4.000 10 000 7.000 4 000 3 000 16000 8.000 9.000 9000 1400 1.0 0000 0000 0.000 0000 0000 0000 0.000 0000 0.000 Tksfortm: Untr'ansfored Rank I.Taifed bIomak Covc-mg/L Mean N-Mea Means minl Mal CV*/. N sum Crtkal mie" N-Mean D.-Contr I 23400 1.0000 23400 22000 32.000 10.392 t0 23.533 1.0000 600 2 O.500 1.0035 23.500 25 000 32.000 7.976 10 103.50 7500 28.533 10000 800 28 700 10106 28.700 25000 33000 7386 10 101.50 7500 28.M33 1.0000 l000 26600 09366 26600 22.000 31000 10.211 10 3450 75.00 26600 09322
- 1200 7.500 0.2641 7.500 3000 16.000 51.544 I0 5500 75.00 7.500 0.2629 "1400 0100 00035 0100 0000 1000 316.222 10 5500 7500 0100 00035 AuxdLary Tests Stidstc Critical Skew Kan Kolmogorov D Test m,+dcalt non-normal dxstrbuton (p <- 0 01) t10093987 1 035 0.37210747 1 60743703 Bertdees Test ndicates unewual vanances (p - 5 03E.-06) 32366781 150863 t7 I Hypothes Test(l-t*ai. 0.01 NOEC LOEC CbV TU Stet's Many-One Rank Test 1000 1200 109544512 Linear Interpolaton (S0 Resamples)
Point mg/L SD 95% CL Skew IC05 947.56207 137069434 475633205 101293162 -2.3107 iCI0 100963351 33701626 932916667 102597968 -30218 ICI5 102457243 9.34834907 10056456 1038 96952 -02116 10 1C20 1039.51134 8.5033028 1022.8989 1052.59962 -00133 09 IC25 105445026 802436167 10401522 106583573 00784 IC40 109916702 80011814 108569236 111716361 04499 0.8 ICSo 112914485 912420599 111431148 1152.92427 06101 07 S05 0
" 0.4
- 03 0.2 01 00
-01 0 500 1000 ism Dose mgI.
Dose-Respoese Plot M
8-.06-02.xis
Environmental Testing Solutions, LLC Statistical Analyses Used for PMSD calculation only. Cerlodapbula Survival and Reproduction Test-Reproduction Start Dat- 8/6/02 Test fD- CdNaCICFR Sample ID" End Date: 8/13/02 Lab I* REF-RefToxicant ETS-Env. TestugSolutions Sample Type:
Sample Date NACL-Sodiwm chloride Protocol. EPAF 9 1-EPA Freshwater Test Species CD-Cenodaphma dubia Comments Conc-ing/L 1 2 3 4 5 6 7 D-Contt1l 27 000 8 9 10 29.000 29 000 30 000 22 000 26 000 32 000 29000 32 000 600 29000 32000 31.000 28 000 27000 25000 30000 30000 800 28 000 26.000 28000 27000 28 000 28 000 33000 28 000 28 000 29.000 28.000 32.000 1000 26000 22.000 30000 25 000 31.000 26000 24000 28.000 1200 5000 28000 25000 26000 4000 10000 7.000 4000 3000 16000 8000 9.000 1400 1000 0000 0000 9000 0.000 0000 0000 0000 0000 0000 0000 Cone-mg/L Transform: Untransformed Mean N-Mean Mean Min 1-Tailed Max CV% N t-Stat D-Control 28 400 Critical MSD 1 0000 28 400 22.000 32 000 600 10392 10 28500 10035 28500 25.000 ' 32000 7976 10 ,-0085 800 28700 10106 28700 2.287 2687 25000 33000 7886 10 1000 26600 09366 -0255 2.287 2.687 26600 22000 31.000 10211 1200 10 1532 2.287 2687 7.500 02641 7500 3000 16000 51.544 10 17786 1400 0.100 00035 2287 2.687 0100 0000 1.000 316228 10 24084 2.287 2.687 Auxiliary Tests Kolmogomv D Test indicates non-normal distribution Statistic Cntical (p <- 0 01) Skew Kurt 1.10093987 1 035 Bartlett's Test indicates unequal variances (p - 5 03E-06) 037210747 1 60743708 Hypothesis Test (1-tail, 0 05) 32366787 150863171 NOEC LOEC ChV TU Dunnett's Test MSDu MSDp MSB MSE 1000 1200 F-Prob df 1095.44512 268694591 009461077 162862667 69037037 2.2E-35 5,54 08-06-02.xls
.Environmental Testing Solutions, LLC Pal* 5 oI 7 EvrmnlTtn tiCPae57 --
CdNaCICR Test Number. 2 MHS Control Chemistry: 1\
Parameter Control Batch:
pHo(S.U) \.01
- 8. go3ck LA.LQd e- XL cLAZO.
DO -Q 4 J (mg/L) 2 Conductivity
&J -
(gtmhos/cm) 5-1 c0dJ.
Alkalinity (mg CaC0 3/L) ______
Hardness (mg CaCO 3/L) _" _
Acceptance Criteriafor Daily Chemistry:
Acceptance Criteria Concentration (dilutions must be remade if the conductivity falls outside the acceptable range)
CONTROL 280 - 360 600 mg NaCLTL 1390 - 1590 800 mg NaCIIL 1780 - 1970 1000 mg NaCI/L 2190 - 2370 1200 mg NaClIL 2550 - 2740 1400 mg NaCUL 2910 - 3160 NaCI Stock 110500 -116300
Vage b ot /
1nwirnnmpntial Testing Solutions. LLCPaeb0I MdaClCR Test Number. Zr flaiv Cm %*rJ ..--- J-_____Day_
Concentration Parameter012 PH (S.U.) G.q 19,7 .0580 CONTROL Conductivity 3 Temperature -q2.L ~ ~. r.
pH (S.U.) 151 13X 600 mg NaCIIL Conductivity H418 Temperature -Z4.-2-. 7.?A pH (S.U.) 8,1(0 DO (mg/L) ~ Z &I .0 800 mg NaCIIL Conductivity (Vmhos/cm) 1 )20 Temperature
__ __ __ C) 2..2. aL. 2'4. -L., 24S'L pH (S.U.) 1z 81
- a. 615 :5 55 .
DO (mgi'L) Co S-0 1000 mg NaCIJL Conductivity (iinhos/cm) z'z'] ZZcq Temperature HZq.'
- 2qq5 7zi ~ ~
pH(S.U.) A.11 f.lq e. IA 0 1, a1L .0 DO (mg/'L) . 4 8 1200 Ing NaCIJL Conductivity (txnhos/cm) z 59 1 EO(1 Temperature 24--z. A0 'b . v4.Z 2.
pH (S.U.) .'4. 2 . i0 1400 mg NaCIIL' Conductivity 1(pmlios/cm)
Temperature 21 zq.' zq.s ZV7. 7.L. 1-n~
I CC)
I_____ ;
initial II Final IInitial Final I Initial 11 Final I IOq'io I I0Eo 0 ~I 0 10 0
Environmental Testinz Solutions, LLC Page 7 ot 7 Environmental Testin2 Solutions, LLC Page 7 o17 CdNaCICR Test Number- 2S Da Concentration Parameter 3 4 5 6 pH (S.U.) .0, 8.0 I 2 06 t .0 .
600NTROCL Conductivity Tempertue DO (mS,) 8.1 6. I
.9**Z - 8. 8.0. 40, 800 mg NaCIOL Te~o~mpeatr Conductivity 021 .*%.
- Zs ,lvi-. 312.Z5 5 Te m p e r a t ur e 1 2 24 .P .
pH(S.U.) . lo e.S.i 6 el3t57 8.0.0 G.1
(_imnios/ci) Iq5 (qqIZ
- Ilf
________ C)* qG ._._ . _ q2_ _
- S*2.. _ . _ 2." . 2 ._ Z _._2 c ) 2z 6. , z2 . 2 (0Q pH (S.U.) -.1. .1' z9 e9.
1000 mg NaCI/L Conductivity 2ieo ZN3
(_ihos/cin) ZI,_t Iti&* 1-4q!65-)1-489
(_pnhos/cm)
Temperature
- 2. Ž.. * "z. _ .(
D. 1e., .
1200 mg NaClfL Conductivity DO(.3) - .2 &. 11.3 98. 2 (lpm.hos/cmn)
Temperature 7.ito .to6 21.10 2z eo
¢C) - L.4 9 2q.2. z 2.zq._
4 1 z.O pH (S.U.) 1f. Z-- .1s"-.0 7 .o . .0 8. 1 1400 mg NaCLIL Conductivity (0c)
Temperature 7-q --- q .2 z q. Z 4. e Initial in I I Final I Initial Final l0q1100 ft'I 0C) 46qqc ~l~oq